Text

Marine Toxicity Study W/Biocide 1001 Using Sheepshead Minnow.
	ML17223B309
Person / Time
Site:	Saint Lucie
Issue date:	05/16/1988
From:	; AQUA SURVEY, INC.
To:	;
Shared Package
ML17223B307	List: ML17223B306; ML17223B308; ML17223B309; ML17223B310; ML17223B311; ML17309A663;
References
	NUDOCS 9111140230
	Download: ML17223B309 (45)
	v • d • e

ATTACHMENT4 BR88-634 JOB ¹ 2029 MARINE TOXICITY STUDY WITH BIOCIDE ¹1001 USING THE MYSID SHRIMP

- iy-.h (X- (

FOR:

MR. LARRY LYONS BETZ LABORATORIES 4636 SUMMERTON RD.

TREVOSEt PA 19047 MAY 16, 1'988 RECUR SURVCV, NC.

499 Aint Breeze Pood Flemington. New Jersey 08822 Telephone (201) 788-8700

( 9111140230 911101 PDR ADOCK 05000335 PDR

$

k 0

FIQUFI SURVEY, IN(:.

BIOMONITORING REPORT FORM ACUTE BIOASSAY Test Substance ID N 1001 Delivered to ASI on April 15, 1388 by Mr. Larry Lyons Cl i ent: Betz Laboratories Facility Location: 4636 Summerton Rd.

Trevose, PA 19047 Laboratory/Investigator (s): AGUA SURYEY INC.

S. Brand J. Mur phy J. Col es K. Zwi zanski 8 ~ Mac Tye Laboratory Certi f ication No. 10303 Bioassay Specification:

Test Material: New Product (Biocide 01001)

Test Type: Static Test Duration (hours) '6 Test Organism: Mysid Shrimp Mysidopsis bahia (common name) (scientific name)

Test Endpoint: LC50 Summary of the Range-Finding and Final Results:

Test Star t ing Date: 04/20/88 Completion Date: 04/22/88 LC50/EC50 (ppm): 1. 0 5. 0 Test Star t ing Dat e: 04/26/88 Comp l et i on Dat e". 04/30/88 LC50/EC50 (ppm): 1. 45 95/ Con f i denc e Int er va1: 1. 22 1. 73 ppm Qualitv Control Summarv:

Control Mortality: 10/

Temperature maintained within +/- 2 deg. C of test temperature?

Yes x No Dissolved Oxygen Levels always greater than 40/ saturation'?

Yes x No Loading factor for all exposure chambers less than or equal to maximum allowed for the test type and temperature? Yes x No Two or more concentrations exhibit a trend deviation? Yes No x 1

Qll 499 Point Breeze Road ~ Flemington, New Jersey 08822 ~ Telephone (201) 788-8700 FAX (201) 788-9165

Test Organism Source: Mul ti-Aquacul ture Systems RECUR SURVEY, IN(.

Shipped by Federal Express Organisms were fed Brine shrimp nauplii through out the test.

Test Organism Acclimation to Dilution Water:

Initial Number af Organisms 500 180 used in the ~

test Total Quarantine/Acclimation Period: 20 days Percentage lost during quarantine <5%

Test Organism Age at Start of Test Young Adults (1.5-2 months)

Test Desian: 9 Number o f Test Conc entrat i ons 5 Number of Replicates/Test Concentration 3 Number of Test Organisms/Repl i cat e 10 Volume a f Test Chamber (l i ter s) 1 Testing Locatian: Aqua Survey's Central Lab Dilution Water:

Dilution Water Source: Laboratory Grade salt water Mortality Data (number dead):

Test Conc entr at i on (Par ts/Mi l l i on)

Expasur e Ti me ICont ral 1.0 R.O 4.0 6,r)

(hrs. ) A B C A B C

B.C A B C A B C A B C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 0 f 0 0 0 0 0 0 0 ~ f f 5 1 5 9 9 9 24 0 1 0 0 0 0 0 1 0 2 1 2 9 10 8 10 10 10 1 1 0 0 0 0 1 1 0 5 '8 9 10 10 10 10 10 10 72 1 1 0 0 0 0 3 1 0 5 10 9 10 10 10 10 10 10 96 1 1 1 0 0 0 3 1 0 6 10 9 10 10 f0 10 10 10 Bioassay Results:

24 hour2.777778e-4 days 0.00667 hours 3.968254e-5 weeks 9.132e-6 months 48 haur 72 haur 96 hour0.00111 days 0.0267 hours 1.587302e-4 weeks 3.6528e-5 months LC50/EC50 (ppm) 2. 75 1 . 60 f.50 Calculation Methad: Litchfield and Wilcaxon Abbreviated Method Does the data satisfy the statistical assumptions of the specified calculation method'7 Yes x No Is the calculated LC50/EC50 valid according to the specifications of the rnethad used?'es x No CLll dW Point Breeze Road ~ Flemington, New Jersey 08822 ~ Telephone (201) 788-8700 FAX (20') 788-%65

Niscellaneous: RECUR SURVEY, IN(.

Was test organism stress observed during the test? No The normally erractic swimming motion of the mysids made difficult to determine if the product was stressing the it shrimpe Were any exposure chambers aerated during the test? No NQTESs

i. The salinity increased in range-finder Oi (RFO i) due to evaporation. However,. there were no observed adverse effects due to this increase in salinity. Subsequent testing was performed with covered exposure chambers.

Certification, Accuracy of report certified by: QC/c Laboratory Direc or Date 1 ture'anager CLll 499 Point Breeze Road ~ Flemington, New Jersey 08822 ~ Telephone (20l) 78S-S700 FAX (201) 788-9165

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AN APPROX I I'IATE LC50 FOR THIS Sl T QF DATA IS 5. 0179'38

>>>>>:>>RESULTS CALCULATED USING 1HE MOVING AVERAGE METHOD SPAN G Lt '50 95 PERCENl CONFIDENCE LIMI 824691E-02 f'.

5.048063 250647 5.99013'0

>>>>>RE 'ULT CALCULATED USING THE PROBIT METHOD I TLf<AT I ONS G H GOODNESS QF F I T PROBAB I L I TY 7 .1280231 1 .8004549 SLQI"E W ~ 7&275 1 95 PERCENT CONFIDENCE LIMITS = 3.6J4403 AND 7.811112 LC50 I'.449429 95 PERCENT CONFIDENCE LIMITS 4. 52286 AND 6. 4 38049 LC1 2. 147266

',i5 I"EF;I'EN CLINf IDENCE LIMIfS 1.204956 AND 2. GBt.t$ 398

ATTACHMENT5 BR88-630 JOB ¹ 2029 MARINE TOXICITY STUDY WITH BIOCIDE ¹100i USING THE SHEEPSHEAD MINNOW C (o~- ( o~ C.3-l FOR:

MR. l 4RRY LYONS BETZ LABORATORIES 4636 SUMMERTQN RD.

TREVOSE, PA i9047 MAY i6. i988 RECUR SURVEY NC.

499 Point Breeze Road Flemington, New Jersey 08822 Tetephone (20l) 788-8700

RQuR SURvCv. ~x(.

BIOMONITORING REPORT FORM - ACUTE BIOASBAY Test Substance lD ¹ 1001 Delivered to ASI on April 15, 1988 by Mr. Lar ry Lyans Cl i ent: Betz Laboratories Facility Location: 4636 Summerton Rd.

Trevose, PA 19047 Laboratory/Investigator(s): AQUA SURVEY INC.

S. Brand J. Murphy J. Cales K. Zwizanski G. MacTye Laboratory Certification No. 10309 Bioassay Specificationt'est Material: New Product (Biocide ¹1001)

Test Type: Static Test Duration(hours): 96 Test Organism: Sheepshead minnow Cger inodon var i egatus (common name) (scienti fic name)

Test Endpoint! LC50 Summary of Range-Finding and F'inal Results:

RF Starting Date: 04/20/88 Completion DaCe: 04/22/88 LC50/EC50 (ppm): 5 10 ppm Test Starting Date: 04/26/88 Completian Date: 04/30/88 LC50/EC50 (ppm): 7.0 95/ Confidence Interval: 6-8 ppm Quality Control Summaries Contral Mortality: 0/

Temperature maintained within +/- 2 deg. C of test temperature?

Yes x No Dissolved Oxygen Level-s always greater than 40/. saturation?

Yes x No Loading factor for all exposure chambers less than or equal ta maximum allowed for 'the test type and temperature? Yes x No Two or mare concentrations exhibit a trend deviation? Yes No x 1

CUl 499 Point Breeze Rood ~ Flemington, New Jersey 08822

'I

~ Telephone (201} 788-8700 FAX (201) 788-9165

FIQUR SURVEY, IN(.

Test Organism Source: Aquatic Research Organisms Shipped by F'ederal Express Organisms were fed a varied diet during quarantine. Nothing during the test.

Test Organism Acclimation to Dilution Water:

Initial Number of Organisms 400 180 used in the test Total Quarantine/Acclimation Period: 18 days Percentage lost during quarantine 0 Test Organism Age at Start of Test 3 n>ontfos Test Desian:

Number of Test Concentrations 5 Number of Replicates/Test Concentration 3 Number of Test Organisms/Replicate 10 Volume of Test Chamber (liters) 15 Testing Location: Aqua Survey's Central Lab Dilution Water:

Dilution Water Source: Laboratory Grade salt water Mortality Data (nupber dead): ~

Test Concentration (Part". /Million)

Exposure Time Control Q,o 4.0 6.0 8.0 10. 0 (hrs.) A B C A B C A B C A B C A B C A B C 0 O 0 0 0 0 0 0 0 O 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 10 10 10 24 0 0 0 0 0 0 0 0 0 0 0 0 6 2 5 10 io io 0 0 0 0 0 0 0 0 0 0 '0 0 10 7 9 10 10 10 0 0 0 0 0 0 0 0 0 0 0 0 10 8 9 10 10 10 96 0 0 0 0 0 0 0 0 0 0 0 0 10 8 9 10 10 10 Bioassay Results!

24 hour2.777778e-4 days 0.00667 hours 3.968254e-5 weeks 9.132e-6 months 48 hour5.555556e-4 days 0.0133 hours 7.936508e-5 weeks 1.8264e-5 months 72 hour8.333333e-4 days 0.02 hours 1.190476e-4 weeks 2.7396e-5 months 96 hour0.00111 days 0.0267 hours 1.587302e-4 weeks 3.6528e-5 months

/

LC50/EC50 (ppm) 7. 7S 7. 10 7. 00 7,00 Calculation Method: Litchfield and Wilcoxon Abbreviated Method Does the data satisfy the statistical assumptions of the speci fied calculation method? Yes x No Is the calculated LC50/EC50 valid according to the speci fications of the method used? Yes x No Clll 499 Foint Breeze Road ~ Flemington, New Jersey 08822 ~ Telephone (201) 788-8700 FAX (201) 788-9165

RQUFI SURVEY, INC.

Misc el 1 an+ouse Was test organism stress observed during the test? Yes At 18 hours2.083333e-4 days 0.005 hours 2.97619e-5 weeks 6.849e-6 months the remaining fish in 10 ppm and the fish in 8 ppm were having di f ficlty swimming. At 24 hours2.777778e-4 days 0.00667 hours 3.968254e-5 weeks 9.132e-6 months all of the fish in 8 ppm were sur face swimming. At 48 hours5.555556e-4 days 0.0133 hours 7.936508e-5 weeks 1.8264e-5 months the remaining fish in 8 ppm were still surface swimming. At 72 hours8.333333e-4 days 0.02 hours 1.190476e-4 weeks 2.7396e-5 months the fish in 6 ppm were sur face swimming.

Were any exposure chambers aerated during the test? No Lenath-Weiaht Data:

30 ORGANISMS WERE RANDOMLY SELECTED AND SACRIF ICED TO OBTAIN THE FOLLOWING DATA:

MEAN WEIGHT IN GRAMS: 0. 39 STANDARD DEVIATION FOR WEIGHT: +/- 0. 09 MEAN LENGTH IN MILLIMETERS: 29. 9 STANDARD DEVIATION FOR LENGTH: +/- 2.09 LOADING FACTOR IN GRAMS/LITER: 0.26 Length of longest measured test organism (mm): 42 4

Length of shortest measured test organism (mm): 33 NOTES:

1. The salinity increased in range-finder ¹1 (RF¹ 1) due to evaporation. However, there were no observed adverse effe=ts due to this increase in salinity. Subsequent testing was performed with covered exposure chambers.

Certification:

Accuracy of report certified by:

Laboratory Director atory Manager Cu ture Manager a CVl 499 Point Breeze Road ~ Flemington, New Jersey 08822 ~ Telephone (201) 788-8700 FAX (201} 788-9165

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PHYSICAL-CHEMICALDATA TEST AKALINITY HARDNESS CONCENTRATION WATER TEMP. DO CONDUCTIVITY (mgrt) (mgrt) SALINITY

% EFFLUENT ('C) PH (ppm) (umhos/cm) as CaCO> as CaCO> (Ppt)

X range s X range 7 s X s X s X A. 0 4. l lO O (gS(N= p. ) $ ,3 (N=~ )O,c~ 7.I5 (w=g (N= ) (N= ) (N=

gl,O

~ttaQ ( g ) 3 (Q )0(~ '7l5 (c) ) q.g

-2.i.O

(~ )g(.g ) 4O (c)- ) 5~(3 )

O.i

( ) ( ) ( ) ( )

(. ) ( ) ( ) ( )

( ) ( ) ( ) ( )

X= Mean N= number of determinations used in calculating s standard deviation 5/ri /r Il the mean and standard deviation AQUA SURVEY, INC.

IENT LOCATION /A ORGANISMS ET UP DATE: I ~> CALIBRATED ON SITE I

DATE ~/ )'TARTING

"~CALIBRATED OTHE STARTXNG TZNE: /'CCPln DOSE LIVE COUNT DEGREES OISSOLVEO OXYGEN NOTES

'Ya C ppm 0 24 48 72 96 0 24 48 72 96 0 24 48 72 96

~ l

i r I> 3g tI lr Sr> '>>

/0 I'4 ICl,>> ) i ~

),g~ ygl ) (r>.,C

..I.

/u lu l d I0 l Di Ol.'~.

~

v r

/( 22 3J v 3l IC jO /0 Alt) 0,7 ta/

jo 330 I< 10 I~ (c ~4 S.G ig /v IO jo /o J.5 5 rp Jd lo /o 3I5 5"p

~ 1 i0 I~ 10 /o )g 4r 7 N T'CN

)Q lO )0 o )j fi f /Ir'li s iV ld li 2I.S ~ I i ~

/o pl 5 > /(l,/,

Kz/j.n .>c y~ u~,)Ir ~ >~ gn y~q r.:-. 8~ g~ r~ qH c)v pi )I Aeration started Acclimabion initial number organisms clasped hours clasped cycles time acclimated, in hours temperature range AQUA SURVEY, INC.

deaths P.O. Box 46 Rosemont, New Jersey 08556

CLIENT

~e t < LOCATION ~r"'- fa 'RGANISM& (E S V'0 i

i.(Z P~'~

SET UP DATE: ((~ ~ CALIBRATED ON SITF I

STARTING DATE CALIBRATED OTHE STARTING TINE;~UO DOSE LIVE COUNT DEGREES DISSOLVED OXYGEN NOTES C pplll 0 24 48 72 96 0 24 48 ?2 96 0 24 48 72 96 l(,(

'.,J

~ -J~ri 5'((<'/-Via=

.>o > .g) I/I sg~ f32 .92 54 5"q

~ ~ r sar t~s i6 /Di jg (I 37'5 :~).s >> v c> >.g I'.s

<

D~r (rrrr( rr )

rC I')i n g

2l0 (I

go,G Kz(:.r (8 ~ ('A J~ e g() Jnl rx'- JL J rr .Us r z

')C 0 Aeration started Acclima hion initial number organisms clasped hearn clasped cycles time acclimated, in hours temperature range AQUA SURVEY) INCs deaths P.O. Box 46 Rosemont, New Jersey 08556.

CLIENT e "Z-DOSE pH Al.'r t.i 0 24 48 72 96 24 48 72 96 i(('q $l(j )

~

P <, 5

) fo "')0 7Rd Ky, <

pe 0 p'].G 20 it 7' 21 >

g.

a./

v'$ 3 )II 5 gc)~~

gc.

D g I.5 g

2t)

I -$

Pd pc~

gg. 7 z()~ ,p/. s gr g'/ +

/Y ss 1' QJS y' ) >.u AQUA SURVEY, INC P.O. Box 46 Rosemont, New Jersey 08556

A-6 CLIENT DOSE pH 0 24 48 72 96 0 24 48 72 96

)So ")Ii(q pg. g $ 0. )

7

'-)

q.,z Y7 yq Y;:x 36.0 JA 8C. l ~'~ JJ'7 d WZjnp, jK AQUA SURVEY, INC.

P.O. Box 46 Rosemont, New Jersey 08556

Test: Or aa sm;

- : $. coscatum M, bahl.a M, menMia A, tonsa V4 v'Cn Hours

~o LC50 EC50

~g 5- '.

Phase;

+

C Cl O

C 0 N suspended particulate

, CLIENT:

~

gal.ry - 7. 7) Ip~

,/jan~- 7.l pp~

~

7?Lw- 7.d y/~

p4 kn.

il 7M ~@i~

OX 2 OX 30K 40K SO% 6QX 7 OX 80 X 90 X 10 OX Percent Dead or Affected

eat Specleo 9th'n <<x.lMoight ln gx.

~ 0 0'ean vt. ln 9x.

Std Gev for Mt. +l-4 4'~

0 ~

LkV 34 0'td

.

Hewn ln. ln xx.

28' 33 Dev for 1n. +l-og6 f.ongest Organ lux: pc/

8o o3( n~ Shorteoc Organism:

82 0' Q o+Q JO 0 3(

0'35 PO r3 0 Q,Q

,34 D'SoL 83 o C> 3 6'po O 03 invoot l gator:

J'J/rx 0'

d)'SI 3l 0 w7 Date o

CLIENT < ~ LOCATION ORGANISM C 44 c)'~

P DATE: CALIBRATED ON SITF STARTING DATE:~l "~ CALIBRATED OTHE STARTING TINE: .).'"/T )g.y DOSE

'Vo 0 i LIVE COUNT 4'24 48 72 96 0 DEGREES C

24 48 72 96 DISSOLVED OXYGEN 0

ppm 24 48 72 96

(.4

,'S

[

NOTES 5

2I)> 7 )

v ID 4 E ~ ) ( v% 4 ) 0) )))

10, > )4g 3 c>t.

gd,O nn rn() g ji't/ " i/

Aeration started

>)i~

/ /gg~ p( /'cclima bion initial number organisms clasped hours clasped cycles time acclimated, in hours temperature range AQUA SURVEY, INC.~

deaths P.O. Box 46

~

Q,D ~a+~

~

Rosernnt, New Jersey 08556

CLIENT DOSE 24 48 72 96 24 48 72 96 3C .) 3?gd w

g./ S,s g7g g -I 2F0 Q$ C 8 '3

.

/6,C 8 .'/

~7 Q 3Si i

</), (i 7> ~> +(g u~ > I- ly pu AQUA SURVEY, INC.

PO. Box 46 Rosemont, New Jersey 08556

CLIENT P

~

DATE.'ALIBRATED STARTING DATE STARTINC TIHEl~b, 3o LOCATION CALIBRATED ON SITE OTHE ORGANISM C vao '

DOSE LIVE COUNT DEGREES DISSOLVED OXYGEN NOTES C ppAl 0 24 48 72 96 0 24 4$ 72 96 0 24 48 72 96 P. 0 i5'liras a ON 7 '7 7 q p.5 ,9

)

7 7 ).Io 2I.O p 2I 1 7 2l. 2/5 o.5 48>. I PP d 4.

/o 7 g.9 &be t"..p y.o g4 d.o 24 PA'. 0 7

,O4 7 0 6 7

'I 7 I

0 c 7 0 'Zz kiN ~ )~ 4>

IIET /ah jo Hq Aeration started~ Acc lima b ion initial number organisms slsspsd houTs clasped oyslss time acclimated, in hours temperature range deaths AQUA SURVEY, INC.

PO. Box 46 Rosemont, New Jersey 08556

CLIENT ORGANISM ~ a DATE'OCATION UP ARTING DATE">

STARTING TI:~r: yu CALIBRATED CALIBRATED vt ON SITE OTHE DOSE LIVE COUNT DEGREES DISSOLVED OXYGEN NOTES C ppAl 0 24 48 72 98 0 24 48 72 96 0 24 48 72 96 7

0 7

0 'ZZ 0 'da eg 22.

7 1

0 I

0 fo <.S

$0 gLO Aeration started Acclimabinn xnitial number organisms alaspsd hours olaapad ayalos time acclimated, in hours temperature range AQUA SURVEYs INC deat'hs P.Q Box 46 Rosernont, New Jersey 08556

OOSE 0 24 48 72 96 0 24 48 72 96 g Z Z8.o pe y 2 .0 pro 2h'. o g$ . o 5 J 7i 0 Z9.0 gfo s .a "f 0 ze.o yx+

3g

~s~ $8 t AQUA SURVEY, INC.

P.O. Box 46 Rosemont, New Jersey 08556

OOSF.

0 24 ,

48 72 96 0 24 48 72 96 g,o >So

~ 0

/go z.o

/8. d z .o s8o ggO 2Efg

/icy gA AQUA SURVEV, lNC P.G Box 48 Rosemont, New Jersey 08556

', lone Teat Pates j/ r

'oat Sgecieo v>r,'c ., jr .5 II Ln xx./Height ln gx.

Hean vt. in gi.

Std Oav for vt. wl>>

32, Q<

Hean ln. ln xs.

o33 Std Dev for ln. +I- 2 ~ >9 0 9<

0'9O Longest Organlsa: p~/

0 3b A~ Shortest Organise:

o 58 Q(

p qQ <90 0 3(r 0'30

,70 A '3 A ~33 6 gQ

,34 0'$2 03 0 'pO 30 0'3 ASI Lnveotl9ator:

t z,g Q a Q o2 Gate ~

,

i'CONC. NUMBER NUMBER PERCENT BINOMIAL J EXPOSED DEAD , DEAD PROB.(%)

10 21 21 100 4-768372E-05 8 21 21 100 4.768372E-05 6 2f 100 4.768372E-05 21 21 100 4.768372E-05 21 100 4.768372E-05 21 0 0 4.768372(-05 BINOMIAL TEST SHOWS THAT 1 AND 2 CAN BE USED AS STATISCALLY SOUND CONSERVATIVE 95 PERCENT CONF IDENCE LIMITS SINCE THE ACTUAL CONF IDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS 39.99391 PERCENT.

AN APPROXIMATE LC50 F'QR THIS SET OF DATA IS 1 . 41 4+f 3 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE NQR THE PROSIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS.

Test Organism; I/ > Ch

.I

g, costatum CQ'"

M, bahia M. menidia A, tonsa

,.. ~I >

~~ Hours

~/ LC50(jp~)

~EC50

'.

Phase; liquid suspended particulate CLTENT:

xPJ /~

5J~ Ci')

10 20K 30K 40K BOX 60K 70K BOX 90K 100 Percent Dead or Affected

ML17223B309

Text

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