ML17223B309

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Marine Toxicity Study W/Biocide 1001 Using Sheepshead Minnow.
ML17223B309
Person / Time
Site: Saint Lucie  NextEra Energy icon.png
Issue date: 05/16/1988
From:
AQUA SURVEY, INC.
To:
Shared Package
ML17223B307 List:
References
NUDOCS 9111140230
Download: ML17223B309 (45)
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Text

ATTACHMENT 4BR88-634JOB¹2029MARINETOXICITYSTUDYWITHBIOCIDE¹1001USINGTHEMYSIDSHRIMP-iy-.h(X-(FOR:MR.LARRYLYONSBETZLABORATORIES 4636SUMMERTON RD.TREVOSEtPA19047MAY16,1'988RECURSURVCV,NC.(9111140230 911101PDRADOCK05000335PDR499AintBreezePoodFlemington.

NewJersey08822Telephone (201)788-8700

$k0 FIQUFISURVEY,IN(:.BIOMONITORING REPORTFORM-ACUTEBIOASSAYTestSubstance IDN1001Delivered toASIonApril15,1388byMr.LarryLyonsClient:BetzLaboratories FacilityLocation:

4636Summerton Rd.Trevose,PA19047Laboratory/Investigator (s):AGUASURYEYINC.S.BrandJ.MurphyJ.ColesK.Zwizanski8~MacTyeLaboratory CertificationNo.10303BioassaySpecification:

TestMaterial:

NewProduct(Biocide01001)TestType:StaticTestDuration(hours)'6TestOrganism:

MysidShrimp(commonname)Mysidopsis bahia(scientific name)TestEndpoint:

LC50SummaryoftheRange-Finding andFinalResults:TestStartingDate:04/20/88Completion Date:04/22/88LC50/EC50 (ppm):1.0-5.0TestStartingDate:04/26/88CompletionDate".04/30/88LC50/EC50 (ppm):1.4595/ConfidenceInterva1:1.22-1.73ppmQualitvControlSummarv:ControlMortality:

10/Temperature maintained within+/-2deg.Coftesttemperature?

YesxNoDissolved OxygenLevelsalwaysgreaterthan40/saturation'?

YesxNoLoadingfactorforallexposurechamberslessthanorequaltomaximumallowedforthetesttypeandtemperature?

YesxNoTwoormoreconcentrations exhibitatrenddeviation?

YesNox1Qll499PointBreezeRoad~Flemington, NewJersey08822~Telephone (201)788-8700FAX(201)788-9165 TestOrganismSource:RECURSURVEY,IN(.Multi-Aquacul tureSystemsShippedbyFederalExpressOrganisms werefedBrineshrimpnaupliithroughoutthetest.TestOrganismAcclimation toDilutionWater:InitialNumberafOrganisms

-500~180usedinthetestTotalQuarantine/Acclimation Period:20daysPercentage lostduringquarantine

<5%TestOrganismAgeatStartofTestYoungAdults(1.5-2months)TestDesian:9NumberofTestConcentrations5NumberofReplicates/Test Concentration 3NumberofTestOrganisms/Repl icate10VolumeafTestChamber(liters)1TestingLocatian:

AquaSurvey'sCentralLabDilutionWater:DilutionWaterSource:Laboratory GradesaltwaterMortality Data(numberdead):ExpasureTime(hrs.)IContralABCTestConcentration(Parts/Million)1.04.0R.OABC*B.CABCABC6,r)ABC000000000000000000002000000000000000000040000000000000000000180f0000000~ff5159992401000001021291081010101100001105'891010101010107211000031051091010101010109611100031061091010f0101010BioassayResults:24hour48haur72haur96hourLC50/EC50 (ppm)2.751.60f.50Calculation Methad:Litchfield andWilcaxonAbbreviated MethodDoesthedatasatisfythestatistical assumptions ofthespecified calculation method'7YesxNoIsthecalculated LC50/EC50 validaccording tothespecifications ofthernethadused?'esxNoCLlldWPointBreezeRoad~Flemington, NewJersey08822~Telephone (201)788-8700FAX(20')788-%65 Niscellaneous:

RECURSURVEY,IN(.Wastestorganismstressobservedduringthetest?NoThenormallyerracticswimmingmotionofthemysidsmadeitdifficult todetermine iftheproductwasstressing theshrimpeWereanyexposurechambersaeratedduringthetest?NoNQTESsi.Thesalinityincreased inrange-finder Oi(RFOi)duetoevaporation.

However,.

therewerenoobservedadverseeffectsduetothisincreaseinsalinity.

Subsequent testingwasperformed withcoveredexposurechambers.

Certification, Accuracyofreportcertified by:QC/cLaboratory DirecorDate1ture'anager CLll499PointBreezeRoad~Flemington, NewJersey08822~Telephone (20l)78S-S700FAX(201)788-9165 N0I b~kc,PHYSICAL-CHEMICAL DATATESTCONOENTRATION WATERTEMP.%EFFLUENT('C)pHXrangesXDO(IIm)AKALINITY CONDUCTIVITY (mgtl)(umhoslcm) asCaCO>range7sXHARDNESS(mgll)SALINITYasCaCOs(PPI)sXQQ.OQl)-,~'ttt3b(N=

g)DOq5<s+(N=

5)-S>Z,t5>o.~4.>~%<5>'.-',~pa.'((gp>,~$34(5)O.ML-4S(5)-~>(5'-g,g$,.pL(5@C'6~4.Io($-)c225-~(5)g>5)<(5)g).g5,Lt.>(5P.Cb>'7((5)pig.355(N=)(5)(g)(g)(5)(5)(N=)()()()()()(N=)()()()()()3(5'p.Q'2XL"ty>o47.2.'t(s)be210/)o.4-t)OAP~-~(5)gyre-S,4$(5)C-~2P.pg.R(5)-',I5,(t3(5g.v~(.o4,.%W(.5)

-s~(.33-(g)~92.(6)()()()()()()()()'O(5)~.~-gQ($)o<~~l.l(5)o.(l5)o0RW~(z)(g)O.OWl3t(5)()o5$4()0.07(5)(5)(3).()()()()().'(6)a,y>g~.(5)L).+~~'5()o~3~H-(g)(3'pc4-l7.31.(3)(3)()()~q,pg)o.ql(Z)235~'+B)4'~l-x'3')(3)()()2),Q3>owf.X=MeanN=numberofdeterminations usedincatcutating themeanandstandarddeviation sstandarddeviation AQUASURVEY,INC.~//r

~/<r/err-g/3~+~PHYSICAL-CHEMICAL DATATESTCONCENTRATION WATERTEMP.%EFFLUENT(oCXrangeDO(ppm)CONDUCTIVITY pH(umhoslcm) sXrange7AKALINITY (mgit)asCaCO>sXHARDNESS(mglt)asCaCO3SALINITY(ppt)sXZ2'c'5(w=

p.)-,I.q~(N=g)0.0'6cA,5(z)~,%,.~5(~)a-Wl 1.t(~=/)-q)2(N=)(N=)(N=)-In(2)O.f(g,5(~)~Q(X=MeanN=numberofdeterminations usedincalculating themeanandstandarddeviation sstandarddeviation dLJ5irAQUASURVEY,INC.

I.DCATIDN JI<u<tfORGANISM~]:l-,CLIENTSETUPDATE'CALIBRATED ONSITE.9l-"(glSTARTINGDATERCALIBRATED OTHESTARTINGTINS:~l<>"

WDOSELIVECOUNT0-24487298DEGREESDISSOLVED OXYGENCppN02448?296024487296NOTESOPIoCO.5pI-/IAIl0/0IoIaer)'7/)30~I3034)7g3b73~y~$)~)AY2(.@>--<~kS6>i'(y,p<>F~.o>'.-~<(0.'(ofa,~,sag(ttCW~ss0d.4<<o"'-'C~(s~sl'ti.,iGu(ulo(0/0/0/r./CiO>O(0I'oi>Ii'k~'3~g,>>;>"-~cgs.~>,))g.,v2g.+z-.>-~6.7C'.5$,a,g((j'/(p(0:/d9GAo'Pg-c'sl;g7'F26(;'.g6.1',e~S(u5II/)gd~oQ.-pa7.Q(l/~5@gfz/jc9,<M:~gyQ(~TPiJl4g~Jc,++~J+Y-rg~4jmJgqAerationstartedAcclimalionsIaspsdhearnslespedoyslssAQUASURVEY,INC.P.O.Box46Rosemont, NewJersey08556initialnumberorganisms timeacclimated, inhourstemperature rangedeaths

)IENTLhKLOCATION""/II.ORGANISM+ETUPDATE:"CALIBRATED ONSITE,S/."U<</n.~>STARTINGDATE:7~~><CALIBRATED OTHESTARTINGTINE:~60DOSELIVECOUNT0'-24487296DEGREES'C024487296DISSOLVED OXYGENpprn02448?296NOTES9.6hIo(C(CiaII;I0g~o"0y.%<.,l~xg6,.(=7~Sg~~L.'IV27].-57/0iv'to/'Iri'0)~f."6Aerationstartedr.(~m.Acclihabion claspedhoursclaspedcyclesAQUASURVEY,INC.P.O.Box46Rosemont, NewJersey08556.initialnumberorganisms timeacclimated, inhourstemperature rangedeaths CLIENTDOSE0244872S62448729693g.5$.5f'.33'.7$-.g7~D)05)Cfwl.(~gK57~(.02y'.5XU.0n~p9.sP'to3g.+gu,O)08'.(~3c~~3'l(J'I$',5AQUASURVEY,INC.PD.Box46Rosemont, NewJersey06556 CLIENTOOSEpH24487286244872963)G9505w$-)-g't0AQUASURVEY,lNLPO.Box46Rosemont, NewJersey08556

.SCGART.~3ex70KE.,b5Se.eeeae.IJJDfftvaa'Utfft'4ie~NeiTestConctionelfe~v5490(ppm)EftCTIttfffgfVtChWOgfIIe'II~~~I~I~I~s~~~~~~I~I.'e'~~e-~'ete,~I~~;I~-I;1I~3~Is~f~seTig,I~1~i~~~Qg..'1~eI'.'I~~Ii~Ie~1+es)L~~~ITTs..lTlI~I":1eeIaIee~'~IveI~~1'~s~I~~Iee~e~I"ei'.s~It15I;I~II~Ia1Ie~iITf)I'tI~~1g~11'~-ailieee~I~iIa~,tteTTTO(DI~III~al~~~~JII~ili.sa~II~a~~It;I~3,e~I~eea~~~'IHt~~'.Ti.Ii~a~~I'a~iIsI~l'~~.11Iaell~,ft3)fs3~e~~'~~~I~J~~IIt,f'~10ttTTTOI~II~'e~e~I~I~~'TJII~~II~sls~~~I,e~II,a~I~~Ifa=~IIiI~IsI~~a~je~'~as~'fIa,,e1;fef3tf~sI~IeIlI'~ie'Ii~I~~~I-JI~tI~~e1~aIs.I1'~I~'3J.'I!eisi~~~eeegt,~I~I"~~i~~i~'~~ea3el~,te~~ee~~eI~~~I~1,~I'~I~ge~IJ~I1st~I~.aa'.IsTItetIeI~~e+leP31lleI~;,.Ieea~III~~1Ie,~1'ee1'1.I3IiIT'eII'QfiI.gI~~~~~Ie~s~.II'I'I~Ie.li.'It~lfIeI~Ie~1~.I~s1-I-iI~1'l~IiII~~IT~e'te~<<Igm~stgTII~31~)IIeIseil',ITII",Iqe7tg1~I*~~Igila~*t~94jTg..I'3g~~~~~~l..J43'laI~'I,~l~astI~,I".~i~IJ~eats'"Ii~.'If)tM~~~1T11'~,es~f31~,II,i.i'iilt(iiITt~~3,-

~~Iil~~Ie~~~~~o~~~~~s~1 CLIENTLOCATIONORGANISM~i

<4iPDATE:CAL!BRATED ONSITE/STARTINGDATE-oCALIBRATED OTHESTARTINGTINE:~4!',F DOSELIVECOUNT0/~.>24487296DEGREESC02448'296DISSOLVED OXYGENpplTI024487296NOTESS~'o"22,u736'ISlr~gP"$30I>~s.o7zIIW'ZIII2~"d,9S,~z~or'/c')1vn,7,/)'-'7JiypPgr-AerationstartedclaspedhoursclaspedcycIelAQUASURVEY,INC,~~PO,Box46Rosemont, NewJersey08556Q/~jA/jAcclimahoninlrlalnnmbarorganisms timeacclimated, inhourstemperature rangedeat'hs CLIENTOOSEpH02448729602448729653.cg-'/3)>cdgG~iy.o~~26>~/QD9..iJ~/~Tc~Sc<1'QUASUilVEY,INC.P.O.Box46Rosemont, NewJersey08556 ICLIENTLOCATlONORGANlSNl4~4~WSTARTlNGDATE:./IfIggStandardToxicantLiveCountOataSheetSTARlINGTINEA'iIe ofObservation

-NuberofSurvivors ExousureCondition Renlicate 0hr>g'hr]],fhr24hr48hr72hr96hrCulturevatercontrol/d.goJoLQ(bIf)PP/.3alOf0/DzQ/0PP~/Qpc)Jr)ac~PPtOID2+'7A~/3.cIPP A-12'4CL!9Ti.OCAT(OH ORGAH(SN8In4ecIA~nw~irE:

gip'!RLSTARTlH6TlllE;Z-gdgQExousureCondition Renl.Dissolved Oxena0hr24hr48hr72hr96hr0hrTeaeraturedereesC24hr48hr72hr96hrCulturevatercontrol"7,c755~.Cg4S/72/,~Zo.OZD.0ppa2<.5'g/2pigply5zo.oppa3,0wl.Pi),Z.-'0.02l.Cg/./W.o>,0ppa'5a(0,z-0.Z2/!'l,s"2o,o.>/..0Zo020.0/0.0aPP2n.f5,q022(.CCM>15za.o6$-0ppaz!.<

CLlENfLOCALlONsTARIIN8ME:!jLZE-ORGANfSNa,kSBRriHGTlNE:2>.3dXMS>inittExuosureConditionRenl.0hr24hr48hr72hr96hr0hr24hr48hr72hr96hrCulturevoter(ontrol+90M~~~cZ2olXPPngo,o$2.ojp,o+2,g8ippnp),cg,r-B.OPPPaWa,c3Zag.3Q.OPPiB.eaC/~40c1g;rg,.(I)crtppnSg tLtllt.IVUMBL'fi IVUIOi.tL".I>

PL'I';t'f:.I'lI'XPOSEDDEAD.DEAD152t.l2t.l1(.>lJ10.J0la<<072.52't.tIJt=BINOMIALTESTSHOWSTHAT2.5AND1tlCANBEGEOASSTATISCALLY SOUNDCONSEF.'VATIVE 95PLRCENTCQI<flDENCELIMITSSINCETHEACTUALCONFIDENCE LEVELASSQCIAlED WITI-ITHESELIMITSIS'i9.996PERCENT.ANAPPROXII'IATELC50FORTHISSlTQFDATAIS5.0179'38B1NOrlIALf'"ROB.(%)5~6743E-0 2.002716E-03 13.15882.002716E-03

~".Si@~E-OSA->>>>>:>>RESULTS CALCULATED USING1HEMOVINGAVERAGEMETHODSPANGLt'5095PERCENlCONFIDENCE LIMIf'.824691E-02 5.048063250647>>>>>RE'ULTCALCULATED USINGTHEPROBITMETHOD5.99013'0 ITLf<ATIONS7G.1280231H1GOODNESSQFFITPROBABILITY.8004549SLQI"EW~7&275195PERCENTCONFIDENCE LIMITS=3.6J4403AND7.811112LC5095PERCENTLC1',i5I"EF;I'EN I'.449429 CONFIDENCE LIMITS2.147266CLINfIDENCELIMIfS1.204956AND2.GBt.t$3984.52286AND6.438049 ATTACHMENT 5BR88-630JOB¹2029MARINETOXICITYSTUDYWITHBIOCIDE¹100iUSINGTHESHEEPSHEAD MINNOWC(o~-(o~C.3-lFOR:MR.l4RRYLYONSBETZLABORATORIES 4636SUMMERTQN RD.TREVOSE,PAi9047MAYi6.i988RECURSURVEYNC.499PointBreezeRoadFlemington, NewJersey08822Tetephone (20l)788-8700 RQuRSURvCv.~x(.BIOMONITORING REPORTFORM-ACUTEBIOASBAYTestSubstance lD¹1001Delivered toASIonApril15,1988byMr.LarryLyansClient:FacilityLocation:

BetzLaboratories 4636Summerton Rd.Trevose,PA19047Laboratory/Investigator(s):

AQUASURVEYINC.S.BrandJ.MurphyJ.CalesK.Zwizanski G.MacTyeLaboratory Certification No.BioassaySpecificationt'est Material:

NewProduct(Biocide¹1001)TestType:StaticTestDuration(hours):

9610309TestOrganism:

Sheepshead minnow(commonname)Cgerinodonvariegatus(scientificname)TestEndpoint!

LC50SummaryofRange-Finding andF'inalResults:RFStartingDate:04/20/88LC50/EC50 (ppm):5-10ppmTestStartingDate:04/26/88Completion DaCe:04/22/88Completian Date:04/30/88LC50/EC50 (ppm):7.095/Confidence Interval:

6-8ppmQualityControlSummaries ContralMortality:

0/Temperature maintained within+/-2deg.Coftesttemperature?

YesxNoDissolved OxygenLevel-salwaysgreaterthan40/.saturation?

YesxNoLoadingfactorforallexposurechamberslessthanorequaltamaximumallowedfor'thetesttypeandtemperature?

YesxNoTwoormareconcentrations exhibitatrenddeviation?

YesNox1CUl499PointBreezeRood~Flemington, NewJersey08822~Telephone (201}788-8700FAX(201)788-9165'I FIQURSURVEY,IN(.TestOrganismSource:AquaticResearchOrganisms ShippedbyF'ederalExpressOrganisms werefedavarieddietduringquarantine.

Nothingduringthetest.TestOrganismAcclimation toDilutionWater:InitialNumberofOrganisms

-400180usedinthetestTotalQuarantine/Acclimation Period:18daysPercentage lostduringquarantine 0TestOrganismAgeatStartofTest3n>ontfosTestDesian:NumberofTestConcentrations 5NumberofReplicates/Test Concentration 3NumberofTestOrganisms/Replicate 10VolumeofTestChamber(liters)15TestingLocation:

AquaSurvey'sCentralLabDilutionWater:DilutionWaterSource:Laboratory GradesaltwaterMortality Data(nupber~dead):ExposureTime(hrs.)ControlABCTestConcentration (Part"./Million)

Q,o6.010.04.08.0ABCABCABCABCABC0O0000000O0000000002000000000000000000040000000000000000000180000000000002001010102400000000000062510ioio0000000000'0010791010100000000000001089101010960000000000001089101010BioassayResults!/LC50/EC50 (ppm)24hour48hour72hour7.7S7.107.0096hour7,00Calculation Method:Litchfield andWilcoxonAbbreviated MethodDoesthedatasatisfythestatistical assumptions ofthespecifiedcalculation method?YesxNoIsthecalculated LC50/EC50 validaccording tothespecifications ofthemethodused?YesxNoClll499FointBreezeRoad~Flemington, NewJersey08822~Telephone (201)788-8700FAX(201)788-9165

Miscel1an+ouseRQUFISURVEY,INC.Wastestorganismstressobservedduringthetest?YesAt18hourstheremaining fishin10ppmandthefishin8ppmwerehavingdifficltyswimming.

At24hoursallofthefishin8ppmweresurfaceswimming.

At48hourstheremaining fishin8ppmwerestillsurfaceswimming.

At72hoursthefishin6ppmweresurfaceswimming.

Wereanyexposurechambersaeratedduringthetest?NoLenath-Weiaht Data:30ORGANISMS WERERANDOMLYSELECTEDANDSACRIFICEDTOOBTAINTHEFOLLOWING DATA:MEANWEIGHTINGRAMS:0.39STANDARDDEVIATION FORWEIGHT:+/-0.09MEANLENGTHINMILLIMETERS:

29.9STANDARDDEVIATION FORLENGTH:+/-2.09LOADINGFACTORINGRAMS/LITER:

0.26Lengthoflongestmeasuredtestorganism(mm):424Lengthofshortestmeasuredtestorganism(mm):33NOTES:1.Thesalinityincreased inrange-finder

¹1(RF¹1)duetoevaporation.

However,therewerenoobservedadverseeffe=tsduetothisincreaseinsalinity.

Subsequent testingwasperformed withcoveredexposurechambers.

Certification:

Accuracyofreportcertified by:Laboratory DirectoratoryManagerCutureManageraCVl499PointBreezeRoad~Flemington, NewJersey08822~Telephone (201)788-8700FAX(201}788-9165 P0I ucL~lysihLSPHYSICAL-CHEMICAL DATATESTCONCENTRATION tin/ATERTEMP.%EFFWENT('C)XrangepHR00(ppm)CONDUCTIVITY (umhos/cm) range7AKALINITY (mg/I)asCaCO>sXsXHARDNESS(mg/I)asCaCO~SALINITY(ppt)sX~.Og(N=5)-&Re'3-~(5)'8,3q.(j)0.(73),)q(I))b.cdg)LC.(~)I"'3)-BIOQ.)Q)-'SO(..2(c(g)~I()()()()()(N=)()()()()()(N=)()()()()()'l(N=5)03Lg.o(g)o-x"l.((g)0.(%)9(g)o.3t1>(9).>~IC3~(5)0('l34.((c-.(g)ilre()()()).3q-(g)0.013(j)00"34.(&(5)-ro~[$'Jg()()()()()()4phi~%(5)(),cl3$.5(o(5)~.'(3.(g)'g,g()()()()()Bi"()0,39()A~~($)c>32).I(~<18biSf8>>(50-;,3g(5)~'4rO3.(5Sitp.().-g,p'()()()()()()()()()g.~(5)o.uX=Mean~2J.O>>0(5)-2y>'f,ogc(5)-g.i()()()cjm5/(/gVN=numberotdeterminations usedincalculating themeanandstandarddewatronsstandarddewationAQUASURVEY,INC.

PHYSICAL-CHEMICAL DATATESTCONCENTRATION WATERTEMP.DOCONDUCTIVITY

%EFFLUENT('C)PH(ppm)(umhos/cm)

XrangesXrange7sXAKALINITY HARDNESS(mgrt)(mgrt)asCaCO>asCaCO>sXSALINITY(Ppt)sXlOOA.0(gS(N=p.)-gl,O~ttaQ(g)-2.i.O(~)g(.g4.l$,3(N=~)O,c~7.I5(w=g3(Q)0(~'7l5(c))q.g)4O(c)-)(N=)(N=)(N=5~(3)O.i()()()()()()()()()()()()()()()()()()()()()()()()()(.)()()()()()()()()()()()()()()()()()()()()()()X=MeanN=numberofdeterminations usedincalculating themeanandstandarddeviation sstandarddeviation 5/ri/rIlAQUASURVEY,INC.

IENTLOCATION/AORGANISMS ETUPDATE:I~>CALIBRATED ONSITEI)'TARTING DATE~/"~CALIBRATED OTHESTARTXNGTZNE:/'CCPlnDOSE'YaLIVECOUNT0-24487296DEGREESC024487296OISSOLVEO OXYGENppm024487296~l;irNOTES/0I'4/ululdI0ICjOI<10I~IOjoJdloI>3gtIlDi/(22/0Alt)jo330(c~4/oJ.5/o3I53Jv3l),g~ygl5rp0,7ta/S.Gig/v5"plrSr>>>i~ICl,>>))~..I.(r>.,CvOl.'~.riV~1i0I~10)QlO)0ldli/o)go)j2I.S/opl54r7fif/Ir'li>/(l,/,NT'CNs~Ii~Kz/j.n.>cy~u~,)Ir~>~gny~qr.:-.8~g~r~qHc)vpi)IAerationstartedAcclimabion claspedhoursclaspedcyclesAQUASURVEY,INC.P.O.Box46Rosemont, NewJersey08556initialnumberorganisms timeacclimated, inhourstemperature rangedeaths LOCATION~r"'-fa'RGANISM&

CLIENT~et<SETUPDATE:((~~CALIBRATED ONSITFISTARTINGDATECALIBRATED OTHESTARTINGTINE;~UOi(ESV'0i.(ZP~'~DOSELIVECOUNT0-24487296DEGREESDISSOLVED OXYGENCpplll02448?296024487296NOTESl(,('.,JI')ingi6/Di2l0.>o>sg~f32jg(I37'5.g)I/I.92:~).s>>vc>>.g545"qI'.srC~~<rsart~sD~r(rrrr(rr)~-J~ri5'((<'/-Via=

(Igo,GKz(:.r(8~('AJ~eg()Jnlrx'-JLJrr.UsrzAerationstarted')C0AcclimahionclaspedhearnclaspedcyclesAQUASURVEY)INCsP.O.Box46Rosemont, NewJersey08556.initialnumberorganisms timeacclimated, inhourstemperature rangedeaths CLIENTe"Z-DOSEpH024487296Al.'rt.i2448729620itg.v'$3Pi(('q<,)fo)II5$l(j)Ky,<5"')0~7Rdpe0p'].G7'21>gc)~~gI.5Pdgc.DgI-$pc~a.//Ygrssy'gg.7z()~2t)g'/+1',p/.sQJS)>.uAQUASURVEY,INCP.O.Box46Rosemont, NewJersey08556 CLIENTA-6DOSEpH024487296024487296q.,zyqY7)So")Ii(qpg.g$0.)'-)7Y;:x36.08C.l~'~JJ'7dWZjnp,JAjKAQUASURVEY,INC.P.O.Box46Rosemont, NewJersey08556 Test:Oraasm;-:$.coscatumM,bahl.aM,menMiaA,tonsaV4v'CnHours~oLC50EC50~g5-+CClOC0N'.Phase;suspended particulate

,CLIENT:OX2OX809010PercentDeadorAffectedOX30K40KSO%6QX7XXOX~gal.ry-7.7)Ip~,/jan~-7.lpp~~7?Lw-7.dy/~p4kn.7M~@i~il eatSpecleo9th'n<<x.lMoight lngx.~00~LkV34.0'eanvt.ln9x.4StdGevforMt.+l-4'~Hewnln.lnxx.28'330'tdDevfor1n.+l-8oo3(og6f.ongestOrganlux:pc/n~ShorteocOrganism:

Qo+Q820'JO03(0'35POr30Q,Q,34D'SoL83oC>36'po0'O033l0w7d)'SIinvootlgator:J'J/rxDateo CLIENT<~LOCATIONPDATE:CALIBRATED ONSITFSTARTINGDATE:~l"~CALIBRATED OTHESTARTINGTINE:.).'"/T)g.yORGANISMC44c)'~DOSE'VoLIVECOUNTDEGREESC0i4'244872960244872965DISSOLVED OXYGENppm024487296(.4,'S[NOTES2I)>7)10,>)4gvID4E~)(v%4)0))))3c>t.gd,Onnrn()g>)i~ji't/"i/Aerationstarted//gg~p(/'cclimabionclaspedhoursclaspedcyclesAQUASURVEY,INC.~~P.O.Box46Rosernnt, NewJersey08556initialnumberorganisms timeacclimated, inhourstemperature rangedeathsQ,D~~a+~

CLIENTDOSE24487296244872963C.)3?gdg./S,swg7gg-I2F0Q$C8.'3/6,C8.'/~7Q3Sii</),(i7>~>+(gu~>I-lypuAQUASURVEY,INC.PO.Box46Rosemont, NewJersey08556 CLIENT~LOCATIONORGANISMCvao'PDATE.'ALIBRATED ONSITESTARTINGDATECALIBRATED OTHESTARTINCTIHEl~b,3oDOSELIVECOUNT0-24487296DEGREESC0244$7296DISSOLVED OXYGENppAl024487296NOTESP.0i5'lirasap7/o7,O477'7771770qON).Io)2I.O2l.2I2/524p.5o.5,9g.948>.I&bet"..py.oPPd4.g4d.oPA'.067'I7c7I00'ZzIIET/ahjoAerationstarted~slsspsdhouTsclaspedoyslssAQUASURVEY,INC.PO.Box46Rosemont, NewJersey08556HqkiN~)~4>Acclimabioninitialnumberorganisms timeacclimated, inhourstemperature rangedeaths CLIENTUPDATE'OCATION CALIBRATED ONSITEORGANISM~aARTINGDATE">CALIBRATED

-OTHESTARTINGTI:~r:yuvtDOSELIVECOUNT0-24487298DEGREESDISSOLVED OXYGENCppAl024487296024487296NOTES7070'ZZ0'daeg7100Ifo$022.gLO<.SAerationstartedalaspsdhoursolaapadayalosAQUASURVEYsINCP.QBox46Rosernont, NewJersey08556Acclimabinn xnitialnumberorganisms timeacclimated, inhourstemperature rangedeat'hs OOSE024487296024487296gZZ8.o2.0peypro2h'.o5J7i0gfo"f0g$.oZ9.0s.aze.oyx+~s~3g$8tAQUASURVEY,INC.P.O.Box46Rosemont, NewJersey08556 OOSF.024,487296024487296g,o>So/go~0/8.dz.oz.os8oggO2Efg/icygAAQUASURVEV,lNCP.GBox48Rosemont, NewJersey08556

',lonej/r'oatSgecieoTeatPatesv>r,'c.,jr.5IILnxx./Height lngx.o3332,Q<Heanvt.ingi.StdOavforvt.wl>>Heanln.lnxs.StdDevforln.+I-2~>90'9O03b09<LongestOrganlsa:

p~/A~ShortestOrganise:

o58pqQQ(<9003(r0'30,70A'3A~336gQ,340'$2030'pOQaQ300'3o2ASILnveotl9ator:

tz,gGate~

,i'CONC.J1086NUMBERNUMBERPERCENTEXPOSEDDEAD,DEAD212110021211002f1002121100211002100BINOMIALTESTSHOWSTHAT1AND2CANBEUSEDASSTATISCALLY SOUNDCONSERVATIVE 95PERCENTCONFIDENCELIMITSSINCETHEACTUALCONFIDENCELEVELASSOCIATED WITHTHESELIMITSIS39.99391PERCENT.ANAPPROXIMATE LC50F'QRTHISSETOFDATAIS1.414+f3BINOMIALPROB.(%)4-768372E-05 4.768372E-05 4.768372E-05 4.768372E-05 4.768372E-05 4.768372(-05 WHENTHEREARELESSTHANTWOCONCENTRATIONS ATWHICHTHEPERCENTDEADISBETWEEN0AND100,NEITHERTHEMOVINGAVERAGENQRTHEPROSITMETHODCANGIVEANYSTATISTICALLY SOUNDRESULTS.

I/>ChCQ'"TestOrganism;

g,costatum.IM,bahiaM.menidiaA,tonsa,..~I>~~Hours~/LC50(jp~)

~EC50'.Phase;liquidsuspended particulate CLTENT:xPJ/~5J~Ci')20K30K40KBOX60K70KPercentDeadorAffected10BOX90K100

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