ML17223B309
| ML17223B309 | |
| Person / Time | |
|---|---|
| Site: | Saint Lucie  |
| Issue date: | 05/16/1988 |
| From: | AQUA SURVEY, INC. |
| To: | |
| Shared Package | |
| ML17223B307 | List: |
| References | |
| NUDOCS 9111140230 | |
| Download: ML17223B309 (45) | |
Text
ATTACHMENT 4 BR88-634 JOB¹2029 MARINE TOXICITY STUDY WITH BIOCIDE¹1001 USING THE MYSID SHRIMP-iy-.h (X-(FOR: MR.LARRY LYONS BETZ LABORATORIES 4636 SUMMERTON RD.TREVOSEt PA 19047 MAY 16, 1'988 RECUR SURVCV, NC.(9111140230 911101 PDR ADOCK 05000335 PDR 499 Aint Breeze Pood Flemington.
New Jersey 08822 Telephone (201)788-8700
$k 0 FIQUFI SURVEY, IN(:.BIOMONITORING REPORT FORM-ACUTE BIOASSAY Test Substance ID N 1001 Delivered to ASI on April 15, 1388 by Mr.Larry Lyons Cl i ent: Betz Laboratories Facility Location: 4636 Summerton Rd.Trevose, PA 19047 Laboratory/Investigator (s): AGUA SURYEY INC.S.Brand J.Mur phy J.Col es K.Zwi zanski 8~Mac Tye Laboratory Certi f ication No.10303 Bioassay Specification:
Test Material: New Product (Biocide 01001)Test Type: Static Test Duration (hours)'6 Test Organism: Mysid Shrimp (common name)Mysidopsis bahia (scientific name)Test Endpoint: LC50 Summary of the Range-Finding and Final Results: Test Star t ing Date: 04/20/88 Completion Date: 04/22/88 LC50/EC50 (ppm): 1.0-5.0 Test Star t ing Dat e: 04/26/88 Comp l et i on Dat e".04/30/88 LC50/EC50 (ppm): 1.45 95/Con f i denc e Int er va1: 1.22-1.73 ppm Qualitv Control Summarv: Control Mortality:
10/Temperature maintained within+/-2 deg.C of test temperature?
Yes x No Dissolved Oxygen Levels always greater than 40/saturation'?
Yes x No Loading factor for all exposure chambers less than or equal to maximum allowed for the test type and temperature?
Yes x No Two or more concentrations exhibit a trend deviation?
Yes No x 1 Qll 499 Point Breeze Road~Flemington, New Jersey 08822~Telephone (201)788-8700 FAX (201)788-9165 Test Organism Source: RECUR SURVEY, IN(.Mul ti-Aquacul ture Systems Shipped by Federal Express Organisms were fed Brine shrimp nauplii through out the test.Test Organism Acclimation to Dilution Water: Initial Number af Organisms-500~180 used in the test Total Quarantine/Acclimation Period: 20 days Percentage lost during quarantine
<5%Test Organism Age at Start of Test Young Adults (1.5-2 months)Test Desian: 9 Number o f Test Conc entrat i ons 5 Number of Replicates/Test Concentration 3 Number of Test Organisms/Repl i cat e 10 Volume a f Test Chamber (l i ter s)1 Testing Locatian: Aqua Survey's Central Lab Dilution Water: Dilution Water Source: Laboratory Grade salt water Mortality Data (number dead): Expasur e Ti me (hrs.)ICont ral A B C Test Conc entr at i on (Par ts/Mi l l i on)1.0 4.0 R.O A B C*B.C A B C A B C 6,r)A B C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 0 f 0 0 0 0 0 0 0~f f 5 1 5 9 9 9 24 0 1 0 0 0 0 0 1 0 2 1 2 9 10 8 10 10 10 1 1 0 0 0 0 1 1 0 5'8 9 10 10 10 10 10 10 72 1 1 0 0 0 0 3 1 0 5 10 9 10 10 10 10 10 10 96 1 1 1 0 0 0 3 1 0 6 10 9 10 10 f0 10 10 10 Bioassay Results: 24 hour2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> 48 haur 72 haur 96 hour0.00111 days <br />0.0267 hours <br />1.587302e-4 weeks <br />3.6528e-5 months <br /> LC50/EC50 (ppm)2.75 1.60 f.50 Calculation Methad: Litchfield and Wilcaxon Abbreviated Method Does the data satisfy the statistical assumptions of the specified calculation method'7 Yes x No Is the calculated LC50/EC50 valid according to the specifications of the rnethad used?'es x No CLll dW Point Breeze Road~Flemington, New Jersey 08822~Telephone (201)788-8700 FAX (20')788-%65 Niscellaneous:
RECUR SURVEY, IN(.Was test organism stress observed during the test?No The normally erractic swimming motion of the mysids made it difficult to determine if the product was stressing the shrimpe Were any exposure chambers aerated during the test?No NQTESs i.The salinity increased in range-finder Oi (RFO i)due to evaporation.
However,.there were no observed adverse effects due to this increase in salinity.Subsequent testing was performed with covered exposure chambers.Certification, Accuracy of report certified by: QC/c Laboratory Direc or Date 1 ture'anager CLll 499 Point Breeze Road~Flemington, New Jersey 08822~Telephone (20l)78S-S700 FAX (201)788-9165 N 0 I b~k c, PHYSICAL-CHEMICAL DATA TEST CONOENTRATION WATER TEMP.%EFFLUENT ('C)pH X range s X DO (I I m)AKALINITY CONDUCTIVITY (mgtl)(umhoslcm) as CaCO>range 7 s X HARDNESS (mgll)SALINITY as CaCOs (PPI)s X QQ.O Ql)-,~'ttt3b(N=g)DOq5<s+(N=
5)-S>Z, t 5>o.~4.>~%<5>'.-',~pa.'((g p>,~$34 (5)O.M L-4S (5)-~>(5'-g,g$,.pL(5@C'6~4.Io ($-)c 2 25-~(5)g>5)<(5)g).g 5,Lt.>(5 P.Cb>'7((5)pig.355 (N=)(5)(g)(g)(5)(5)(N=)()()()()()(N=)()()()()()3(5'p.Q'2 XL"ty>o 47.2.'t (s)be 210/)o.4-t)O A P~-~(5)gyre-S,4$(5)C-~2P.pg.R (5)-', I5,(t 3 (5 g.v~(.o 4,.%W(.5)-s~(.3 3-(g)~9 2.(6)()()()()()()()()'O(5)~.~-gQ($)o<~~l.l(5)o.(l 5)o 0 R W~(z)(g)O.OW l3t (5)()o 5$4()0.07 (5)(5)(3).()()()()().'(6)a,y>g~.(5)L).+~~'5()o~3~H-(g)(3'p c4-l 7.31.(3)(3)()()~q,pg)o.ql (Z)235~'+B)4'~l-x'3')(3)()()2),Q3>o wf.X=Mean N=number of determinations used in catcutating the mean and standard deviation s standard deviation AQUA SURVEY, INC.~//r
~/<r/err-g/3~+~PHYSICAL-CHEMICAL DATA TEST CONCENTRATION WATER TEMP.%EFFLUENT (oC X range DO (ppm)CONDUCTIVITY pH (umhoslcm) s X range 7 AKALINITY (mgit)as CaCO>s X HARDNESS (mglt)as CaCO3 SALINITY (ppt)s X Z2'c'5(w=p.)-, I.q~(N=g)0.0'6 c A,5 (z)~,%,.~5(~)a-Wl 1.t (~=/)-q)2 (N=)(N=)(N=)-In(2)O.f (g,5(~)~Q(X=Mean N=number of determinations used in calculating the mean and standard deviation s standard deviation d LJ 5 ir AQUA SURVEY, INC.
I.DCATIDN JI<u<t f ORGANISM~]:l-, CLIENT SET UP DATE'CALIBRATED ON SITE.9l-"(gl STARTING DATE R CALIBRATED OTHE STARTING TINS:~l<>" W DOSE LIVE COUNT 0-24 48 72 98 DEGREES DISSOLVED OXYGEN C ppN 0 24 48?2 96 0 24 48 72 96 NOTES OP Io C O.5p I-/IAI l 0/0 Io Ia er)'7/)30~I3 0 34)7g 3b 73~y~$)~)A Y2 (.@>--<~k S 6>i'(y,p<>F~.o>'.-~<(0.'(o fa,~,sa g (t tC W~ss 0 d.4<<o"'-'C~(s~s l'ti., iG u (u lo (0/0/0/r./C iO>O (0 I'o i>I i'k~'3~g,>>;>"-~c gs.~>,))g., v2 g.+z-.>-~6.7 C'.5$,a, g ((j'/(p (0:/d 9GA o'P g-c'sl;g7'F2 6 (;'.g 6.1',e~S (u5 I I/)gd~o Q.-pa 7.Q (l/~5@g fz/jc 9,<M:~gy Q(~TP i Jl4g~Jc,++~J+Y-r g~4 jm Jgq Aeration started Acc lima lionsIaspsd hearn slesped oyslss AQUA SURVEY, INC.P.O.Box 46 Rosemont, New Jersey 08556 initial number organisms time acclimated, in hours temperature range deaths
)IENT LhK LOCATION""/II.ORGANISM+ET UP DATE: " CALIBRATED ON SITE , S/."U<</n.~>STARTING DATE: 7~~><CALIBRATED OTHE STARTING TINE:~60 DOSE LIVE COUNT 0'-24 48 72 96 DEGREES'C 0 24 48 72 96 DISSOLVED OXYGEN pprn 0 24 48?2 96 NOTES 9.6 h Io (C (C ia II;I 0 g~o" 0 y.%<., l~xg 6,.(=7~Sg~~L.'I V2 7].-5 7/0 iv'to/'I ri'0)~f." 6 Aeration started r.(~m.Acclihabion clasped hours clasped cycles A QUA SURVEY, INC.P.O.Box 46 Rosemont, New Jersey 08556.initial number organisms time acclimated, in hours temperature range deaths CLIENT DOSE 0 24 48 72 S6 24 48 72 96 93 g.5$.5 f'.3 3'.7$-.g 7~D)05)Cf wl.(~g K5 7~(.0 2y'.5 XU.0 n~p 9.s P't o 3g.+gu,O)0 8'.(~3 c~~3'l (J'I$',5AQUA SURVEY, INC.PD.Box 46 Rosemont, New Jersey 06556 CLIENT OOSE pH 24 48 72 86 24 48 72 96 3)G 950 5w$-)-g't 0 AQUA SURVEY, lNL PO.Box 46 Rosemont, New Jersey 08556
.SC GART.~3e x 70 KE., b 5Se.ee e ae.IJJDf ft va a'Ut fft'4 i e~Nei Test Conc tion elf e~v 5490 (ppm)Eft CTI ttf ffgf Vt Ch W Ogf I I e'I I~~~I~I~I~s~~~~~~I~I.'e'~~e-~'e t e ,~I~~;I~-I;1 I~3~I s~f~s e Ti g, I~1~i~~~Qg..'1~e I'.'I~~Ii~I e~1+es)L~~~I TT s..lT l I~I": 1 e e I a I e e~'~I v e I~~1'~s~I~~I e e~e~I" e i'.s~I t 15 I;I~I I~I a 1 I e~i IT f)I't I~~1 g~11'~-ail i e e e~I~i I a~, tt e TTT O (D I~I I I~a l~~~~J I I~i li.s a~I I~a~~It;I~3, e~I~ee a~~~'I Ht~~'.Ti.I i~a~~I'a~i I s I~l'~~.11 Ia el l~, ft 3)fs 3~e~~'~~~I~J~~I I t,f'~1 0 tt TTT O I~I I~'e~e~I~I~~'TJ I I~~I I~sl s~~~I, e~I I, a~I~~I fa=~I I i I~I s I~~a~j e~'~as~'f I a,, e 1;fe f 3tf~s I~I e I l I'~ie'I i~I~~~I-JI~t I~~e 1~a I s.I 1'~I~'3 J.'I!eisi~~~e ee gt,~I~I"~~i~~i~'~~e a 3 e l~, t e~~e e~~e I~~~I~1,~I'~I~g e~I J~I 1st~I~.aa'.Is T I tet I e I~~e+le P31 ll e I~;,.I e e a~I I I~~1 I e,~1'e e 1'1.I 3 I iIT'e I I'Qfi I.g I~~~~~I e~s~.I I'I'I~I e.l i.'I t~lf I e I~I e~1~.I~s 1-I-i I~1'l~I i I I~~I T~e't e~<<I gm~s tgT I I~31~)I Ie Is e il',I TII" ,Iq e 7 tg 1~I*~~I gila~*t~94 j Tg..I'3 g~~~~~~l..J43'la I~'I ,~l~as t I~, I".~i~IJ~eats'"Ii~.'I f)t M~~~1T 11'~,e s~f 31~, I I, i.i'iil t(ii I Tt~~3,-
~~I il~~I e~~~~~o~~~~~s~1 CLIENT LOCATION ORGANISM~i
<4i P DATE: CAL!BRATED ON SITE/STARTING DATE-o CALIBRATED OTHE STARTING TINE:~4!',F DOSE LIVE COUNT 0/~.>24 48 72 96 DEGREES C 0 24 48'2 96 DISSOLVED OXYGEN pplTI 0 24 48 72 96 NOTES S~'o" 22,u 73 6'I Slr~gP"$30 I>~s.o 7 zII W'ZIII 2~" d,9 S,~z~o r'/c')1 v n, 7,/)'-'7 JiypPgr-Aeration started clasped hours clasped cycIel AQUA SURVEY, INC,~~PO, Box 46 Rosemont, New Jersey 08556 Q/~jA/j Acclimah on inlrlal nnmbar organisms time acclimated, in hours temperature range deat'hs CLIENTOOSE pH 0 24 48 72 96 0 24 48 72 96 53.c g-'/3)>cd gG~i y.o~~26>~/Q D 9..i J~/~Tc~Sc<1'QUA SUilVEY, INC.P.O.Box 46 Rosemont, New Jersey 08556 I CLIENT LOCATlON ORGANlSN l 4~4~W STARTlNG DATE:./I f I gg Standard Toxicant Live Count Oata Sheet STARlING TINEA'iIe of Observation
-Nu ber of Survivors Exousure Condition Renlicate 0 hr>g'hr]],f hr 24 hr 48 hr 72 hr 96 hr Culture vater control/d.go Jo LQ (b I f)PP/.3 a lO f0/D zQ/0 PP~/Q pc)J r)ac~PP tO ID 2+'7 A~/3.c I PP A-12'4 CL!9T i.OCAT(OH ORGAH(SN 8 In 4 e c I A~nw~irE: g i p'!RL STARTlH6 TlllE;Z-gdgQ Exousure Condition Renl.Dissolved Ox en a 0 hr 24 hr 48 hr 72 hr 96 hr 0 hr Tea erature de rees C 24 hr 48 hr 72 hr 96 hr Culture vater control"7, c7 55~.C g4 S/7 2/,~Zo.O ZD.0 ppa 2<.5'g/2 pi g ply 5 zo.o ppa 3,0 wl.Pi), Z.-'0.0 2 l.C g/./W.o>,0 ppa'5a (0, z-0.Z 2/!'l,s" 2o,o.>/..0 Zo 0 20.0/0.0 a PP 2 n.f 5,q 0 2 2(.C CM>1 5 za.o 6$-0 ppa z!.<
CLlENf LOCAL lON sTARIIN8 ME:!j LZE-ORGANfSNa, k SBRriHG TlNE: 2>.3dXM S>init t Exuosur e Condi tion Renl.0 hr 24 hr 48 hr 72 hr 96 hr 0 hr 24 hr 48 hr 72 hr 96 hr Culture voter (ontrol+90 M~~~c Z2 o l X PPn go,o$2.o jp,o+2,g 8i ppn p),c g, r-B.O PP Pa Wa,c 3Za g.3 Q.O PP iB.e a C/~40 c1 g;rg,.(I)crt ppn Sg t Ltl lt.IVUMBL'fi IVUIOi.tL".I>
PL'I';t'f:.I'l I'XPOSED DEAD.DEAD 15 2t.l 2t.l 1(.>l J 10.J0 la<<0 7 2.5 2't.t IJ t=BINOMIAL TEST SHOWS THAT 2.5 AND 1tl CAN BE GEO AS STATISCALLY SOUND CONSEF.'VATIVE 95 PLRCENT CQI<f lDENCE LIMITS SINCE THE ACTUAL CONFIDENCE LEVEL ASSQCIAlED WITI-I THESE LIMITS IS'i9.996 PERCENT.AN APPROX I I'IATE LC50 FOR THIS Sl T QF DATA IS 5.0179'38 B1NOrl I AL f'"ROB.(%)5~6743E-0 2.002716E-03 13.1588 2.002716E-03
~".S i@~E-OS A->>>>>:>>RESULTS CALCULATED USING 1HE MOVING AVERAGE METHOD SPAN G Lt'50 95 PERCENl CONFIDENCE LIMI f'.824691E-02 5.048063 250647>>>>>RE'ULT CALCULATED USING THE PROBIT METHOD 5.99013'0 I TLf<AT I ONS 7 G.1280231 H 1 GOODNESS QF F I T PROBAB I L I TY.8004549 SLQI" E W~7&275 1 95 PERCENT CONFIDENCE LIMITS=3.6J4403 AND 7.811112 LC50 95 PERCENT LC1',i5 I"EF;I'EN I'.449429 CONFIDENCE LIMITS 2.147266 CLINf IDENCE LIMI fS 1.204956 AND 2.GBt.t$398 4.52286 AND 6.4 38049 ATTACHMENT 5 BR88-630 JOB¹2029 MARINE TOXICITY STUDY WITH BIOCIDE¹100i USING THE SHEEPSHEAD MINNOW C (o~-(o~C.3-l FOR: MR.l 4RRY LYONS BETZ LABORATORIES 4636 SUMMERTQN RD.TREVOSE, PA i9047 MAY i6.i988 RECUR SURVEY NC.499 Point Breeze Road Flemington, New Jersey 08822 Tetephone (20l)788-8700 RQuR SURvCv.~x(.BIOMONITORING REPORT FORM-ACUTE BIOASBAY Test Substance lD¹1001 Delivered to ASI on April 15, 1988 by Mr.Lar ry Lyans Cl i ent: Facility Location: Betz Laboratories 4636 Summerton Rd.Trevose, PA 19047 Laboratory/Investigator(s):
AQUA SURVEY INC.S.Brand J.Murphy J.Cales K.Zwizanski G.MacTye Laboratory Certification No.Bioassay Specificationt'est Material: New Product (Biocide¹1001)Test Type: Static Test Duration(hours):
96 10309 Test Organism: Sheepshead minnow (common name)Cger inodon var i egatus (scienti fic name)Test Endpoint!LC50 Summary of Range-Finding and F'inal Results: RF Starting Date: 04/20/88 LC50/EC50 (ppm): 5-10 ppm Test Starting Date: 04/26/88 Completion DaCe: 04/22/88 Completian Date: 04/30/88 LC50/EC50 (ppm): 7.0 95/Confidence Interval: 6-8 ppm Quality Control Summaries Contral Mortality:
0/Temperature maintained within+/-2 deg.C of test temperature?
Yes x No Dissolved Oxygen Level-s always greater than 40/.saturation?
Yes x No Loading factor for all exposure chambers less than or equal ta maximum allowed for'the test type and temperature?
Yes x No Two or mare concentrations exhibit a trend deviation?
Yes No x 1 CUl 499 Point Breeze Rood~Flemington, New Jersey 08822~Telephone (201}788-8700 FAX (201)788-9165'I FIQUR SURVEY, IN(.Test Organism Source: Aquatic Research Organisms Shipped by F'ederal Express Organisms were fed a varied diet during quarantine.
Nothing during the test.Test Organism Acclimation to Dilution Water: Initial Number of Organisms-400 180 used in the test Total Quarantine/Acclimation Period: 18 days Percentage lost during quarantine 0 Test Organism Age at Start of Test 3 n>ontfos Test Desian: Number of Test Concentrations 5 Number of Replicates/Test Concentration 3 Number of Test Organisms/Replicate 10 Volume of Test Chamber (liters)15 Testing Location: Aqua Survey's Central Lab Dilution Water: Dilution Water Source: Laboratory Grade salt water Mortality Data (nupber~dead): Exposure Time (hrs.)Control A B C Test Concentration (Part"./Million)Q,o 6.0 10.0 4.0 8.0 A B C A B C A B C A B C A B C 0 O 0 0 0 0 0 0 0 O 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 10 10 10 24 0 0 0 0 0 0 0 0 0 0 0 0 6 2 5 10 io io 0 0 0 0 0 0 0 0 0 0'0 0 10 7 9 10 10 10 0 0 0 0 0 0 0 0 0 0 0 0 10 8 9 10 10 10 96 0 0 0 0 0 0 0 0 0 0 0 0 10 8 9 10 10 10 Bioassay Results!/LC50/EC50 (ppm)24 hour2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> 48 hour 72 hour8.333333e-4 days <br />0.02 hours <br />1.190476e-4 weeks <br />2.7396e-5 months <br /> 7.7S 7.10 7.00 96 hour0.00111 days <br />0.0267 hours <br />1.587302e-4 weeks <br />3.6528e-5 months <br /> 7,00 Calculation Method: Litchfield and Wilcoxon Abbreviated Method Does the data satisfy the statistical assumptions of the speci fied calculation method?Yes x No Is the calculated LC50/EC50 valid according to the speci fications of the method used?Yes x No Clll 499 Foint Breeze Road~Flemington, New Jersey 08822~Telephone (201)788-8700 FAX (201)788-9165
Misc el 1 an+ouse RQUFI SURVEY, INC.Was test organism stress observed during the test?Yes At 18 hours2.083333e-4 days <br />0.005 hours <br />2.97619e-5 weeks <br />6.849e-6 months <br /> the remaining fish in 10 ppm and the fish in 8 ppm were having di f ficlty swimming.At 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> all of the fish in 8 ppm were sur face swimming.At 48 hours5.555556e-4 days <br />0.0133 hours <br />7.936508e-5 weeks <br />1.8264e-5 months <br /> the remaining fish in 8 ppm were still surface swimming.At 72 hours8.333333e-4 days <br />0.02 hours <br />1.190476e-4 weeks <br />2.7396e-5 months <br /> the fish in 6 ppm were sur face swimming.Were any exposure chambers aerated during the test?No Lenath-Weiaht Data: 30 ORGANISMS WERE RANDOMLY SELECTED AND SACRIF ICED TO OBTAIN THE FOLLOWING DATA: MEAN WEIGHT IN GRAMS: 0.39 STANDARD DEVIATION FOR WEIGHT:+/-0.09 MEAN LENGTH IN MILLIMETERS:
29.9 STANDARD DEVIATION FOR LENGTH:+/-2.09 LOADING FACTOR IN GRAMS/LITER:
0.26 Length of longest measured test organism (mm): 42 4 Length of shortest measured test organism (mm): 33 NOTES: 1.The salinity increased in range-finder
¹1 (RF¹1)due to evaporation.
However, there were no observed adverse effe=ts due to this increase in salinity.Subsequent testing was performed with covered exposure chambers.Certification:
Accuracy of report certified by: Laboratory Director atory Manager Cu ture Manager a CVl 499 Point Breeze Road~Flemington, New Jersey 08822~Telephone (201)788-8700 FAX (201}788-9165 P 0 I u cL~lysi hL S PHYSICAL-CHEMICAL DATA TEST CONCENTRATION tin/ATER TEMP.%EFFWENT ('C)X range pH R 00 (p pm)CONDUCTIVITY (umhos/cm) range 7 AKALINITY (mg/I)as CaCO>s X s X HARDNESS (mg/I)as CaCO~SALINITY (ppt)s X~.O g(N=5)-&Re'3-~(5)'8,3q.(j)0.(73),)q (I))b.cd g)LC.(~)I"'3)-BIO Q.)Q)-'SO (..2(c (g)~I ()()()()()(N=)()()()()()(N=)()()()()()'l(N=5)03L g.o(g)o-x"l.((g)0.(%)9(g)o.3 t 1>(9).>~IC 3~(5)0 ('l3 4.((c-.(g)ilre ()()()).3q-(g)0.013 (j)00"3 4.(&(5)-ro~[$'Jg ()()()()()()4 p hi~%(5)(),cl3$.5(o (5)~.'(3.(g)'g,g ()()()()()Bi" ()0,39 ()A~~($)c>3 2).I (~<1 8b i Sf 8>>(50-;,3g (5)~'4rO3.(5 Sit p.().-g,p'()()()()()()()()()g.~(5)o.u X=Mean~2J.O>>0 (5)-2y>'f,og c (5)-g.i ()()()cj m 5/(/g V N=number ot determinations used in calculating the mean and standard dewatron s standard dewation AQUA SURVEY, INC.
PHYSICAL-CHEMICAL DATA TEST CONCENTRATION WATER TEMP.DO CONDUCTIVITY
%EFFLUENT ('C)PH (ppm)(umhos/cm)
X range s X range 7 s X AKALINITY HARDNESS (mgrt)(mgrt)as CaCO>as CaCO>s X SALINITY (Ppt)s X lO O A.0 (gS(N=p.)-gl,O~ttaQ (g)-2.i.O (~)g(.g 4.l$,3 (N=~)O,c~7.I5 (w=g 3 (Q)0(~'7l5 (c))q.g)4O (c)-)(N=)(N=)(N=5~(3)O.i ()()()()()()()()()()()()()()()()()()()()()()()()()(.)()()()()()()()()()()()()()()()()()()()()()()X=Mean N=number of determinations used in calculating the mean and standard deviation s standard deviation 5/ri/r IlAQUA SURVEY, INC.
IENT LOCATION/A ORGANISMS ET UP DATE: I~>CALIBRATED ON SITE I)'TARTING DATE~/"~CALIBRATED OTHE STARTXNG TZNE:/'CCPln DOSE'Ya LIVE COUNT 0-24 48 72 96 DEGREES C 0 24 48 72 96 OISSOLVEO OXYGEN ppm 0 24 48 72 96~l;i r NOTES/0 I'4/u lu l d I0 IC jO I<10 I~IO jo Jd lo I>3g tI l Di/(22/0 Alt)jo 330 (c~4/o J.5/o 3I5 3J v 3l),g~ygl 5 rp 0,7 ta/S.G ig/v 5"p lr Sr>>>i~ICl,>>))~..I.(r>.,C v Ol.'~.r iV~1 i0 I~10)Q lO)0 ld li/o)g o)j 2I.S/o pl 5 4r 7 fi f/Ir'li>/(l,/, N T'CN s~I i~Kz/j.n.>c y~u~,)Ir~>~gn y~q r.:-.8~g~r~qH c)v pi)I Aeration started Acclimabion clasped hours clasped cycles AQUA SURVEY, INC.P.O.Box 46 Rosemont, New Jersey 08556 initial number organisms time acclimated, in hours temperature range deaths LOCATION~r"'-fa'RGANISM&CLIENT~e t<SET UP DATE: ((~~CALIBRATED ON SITF I STARTING DATE CALIBRATED OTHE STARTING TINE;~UO i (E S V'0 i.(Z P~'~DOSE LIVE COUNT 0-24 48 72 96 DEGREES DISSOLVED OXYGEN C pplll 0 24 48?2 96 0 24 48 72 96 NOTES l(,('.,J I')i n g i6/Di 2l0.>o>sg~f32 jg (I 37'5.g)I/I.92:~).s>>v c>>.g 54 5"q I'.s rC~~<r sar t~s D~r (rrrr(rr)~-J~ri 5'((<'/-Via=(I go,G Kz(:.r (8~('A J~e g()Jnl rx'-JL J rr.Us r z Aeration started')C 0 Acclima hion clasped hearn clasped cycles AQUA SURVEY)INCs P.O.Box 46 Rosemont, New Jersey 08556.initial number organisms time acclimated, in hours temperature range deaths CLIENT e"Z-DOSE pH 0 24 48 72 96 Al.'r t.i 24 48 72 96 20 it g.v'$3 P i(('q<,)fo)II 5$l(j)Ky,<5"')0~7Rd pe 0 p'].G 7'21>gc)~~g I.5 Pd gc.D g I-$pc~a.//Y gr ss y'gg.7 z()~2t)g'/+1',p/.s QJS)>.u AQUA SURVEY, INC P.O.Box 46 Rosemont, New Jersey 08556 CLIENT A-6 DOSE pH 0 24 48 72 96 0 24 48 72 96 q.,z yq Y7)So")Ii(q pg.g$0.)'-)7 Y;:x 36.08C.l~'~JJ'7 d WZjnp, JA jK AQUA SURVEY, INC.P.O.Box 46 Rosemont, New Jersey 08556 Test: Or aa sm;-:$.coscatum M, bahl.a M, menMia A, tonsa V4 v'Cn Hours~o LC50 EC50~g 5-+C Cl O C 0 N'.Phase;suspended particulate
, CLIENT: OX 2 OX 80 90 10 Percent Dead or Affected OX 30K 40K SO%6QX 7 X X OX~gal.ry-7.7)Ip~,/jan~-7.l pp~~7?Lw-7.d y/~p4 kn.7M~@i~il eat Specleo 9th'n<<x.lMoight ln gx.~0 0~LkV 34.0'ean vt.ln 9x.4 Std Gev for Mt.+l-4'~Hewn ln.ln xx.28'33 0'td Dev for 1n.+l-8o o3(og6 f.ongest Organ lux: pc/n~Shorteoc Organism: Q o+Q 82 0'JO 0 3(0'35 PO r3 0 Q,Q ,34 D'SoL 83 o C>3 6'po 0'O 03 3l 0 w7 d)'SI invoot l gator: J'J/rx Date o CLIENT<~LOCATION P DATE: CALIBRATED ON SITF STARTING DATE:~l"~CALIBRATED OTHE STARTING TINE:.).'"/T)g.y ORGANISM C 44 c)'~DOSE'Vo LIVE COUNT DEGREES C 0 i 4'24 48 72 96 0 24 48 72 96 5 DISSOLVED OXYGEN ppm 0 24 48 72 96 (.4 ,'S[NOTES 2I)>7)10,>)4g v I D 4 E~)(v%4)0))))3 c>t.gd,O nn rn()g>)i~ji't/" i/Aeration started//gg~p(/'cclima bion clasped hours clasped cycles AQUA SURVEY, INC.~~P.O.Box 46 Rosernnt, New Jersey 08556 initial number organisms time acclimated, in hours temperature range deaths Q,D~~a+~
CLIENT DOSE 24 48 72 96 24 48 72 96 3C.)3?gd g./S,s w g7g g-I 2F0 Q$C 8.'3/6,C 8.'/~7 Q 3Si i</), (i 7>~>+(g u~>I-ly pu AQUA SURVEY, INC.PO.Box 46 Rosemont, New Jersey 08556 CLIENT~LOCATION ORGANISM C vao'P DATE.'ALIBRATED ON SITE STARTING DATE CALIBRATED OTHE STARTINC TIHEl~b, 3o DOSE LIVE COUNT 0-24 48 72 96 DEGREES C 0 24 4$72 96 DISSOLVED OXYGEN ppAl 0 24 48 72 96 NOTES P.0 i5'liras a p 7/o 7 ,O4 7 7'7 7 7 1 7 7 0 q ON).Io)2I.O 2l.2I 2/5 24 p.5 o.5 ,9 g.9 48>.I&be t"..p y.o PP d 4.g4 d.o PA'.0 6 7'I 7 c 7 I 0 0'Zz IIET/ah jo Aeration started~slsspsd houTs clasped oyslss AQUA SURVEY, INC.PO.Box 46 Rosemont, New Jersey 08556 Hq kiN~)~4>Acc lima b ion initial number organisms time acclimated, in hours temperature range deaths CLIENT UP DATE'OCATION CALIBRATED ON SITE ORGANISM~a ARTING DATE">CALIBRATED
-OTHE STARTING TI:~r: yu vt DOSE LIVE COUNT 0-24 48 72 98 DEGREES DISSOLVED OXYGEN C ppAl 0 24 48 72 96 0 24 48 72 96 NOTES 7 0 7 0'ZZ 0'da eg 7 1 0 0 I fo$0 22.gLO<.S Aeration started alaspsd hours olaapad ayalos AQUA SURVEYs INC P.Q Box 46 Rosernont, New Jersey 08556 Acclimabinn xnitial number organisms time acclimated, in hours temperature range deat'hs OOSE 0 24 48 72 96 0 24 48 72 96 g Z Z8.o 2.0 pe y pro 2h'.o 5 J 7i 0 gfo"f 0 g$.o Z9.0 s.a ze.o yx+~s~3g$8 t AQUA SURVEY, INC.P.O.Box 46 Rosemont, New Jersey 08556 OOSF.0 24 , 48 72 96 0 24 48 72 96 g,o>So/go~0/8.d z.o z.o s8o ggO 2Efg/icy gA AQUA SURVEV, lNC P.G Box 48 Rosemont, New Jersey 08556
', lone j/r'oat Sgecieo Teat Pates v>r,'c., jr.5 II Ln xx./Height ln gx.o33 32, Q<Hean vt.in gi.Std Oav for vt.wl>>Hean ln.ln xs.Std Dev for ln.+I-2~>9 0'9O 0 3b 0 9<Longest Organlsa: p~/A~Shortest Organise: o 58 p qQ Q(<90 0 3(r 0'30 ,70 A'3 A~33 6 gQ ,34 0'$2 03 0'pO Q a Q 30 0'3 o2 ASI Lnveotl9ator:
t z,gGate~
, i'CONC.J 10 8 6 NUMBER NUMBER PERCENT EXPOSED DEAD , DEAD 21 21 100 21 21 100 2f 100 21 21 100 21 100 21 0 0 BINOMIAL TEST SHOWS THAT 1 AND 2 CAN BE USED AS STATISCALLY SOUND CONSERVATIVE 95 PERCENT CONF IDENCE LIMITS SINCE THE ACTUAL CONF IDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS 39.99391 PERCENT.AN APPROXIMATE LC50 F'QR THIS SET OF DATA IS 1.41 4+f 3 BINOMIAL PROB.(%)4-768372E-05 4.768372E-05 4.768372E-05 4.768372E-05 4.768372E-05 4.768372(-05 WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER THE MOVING AVERAGE NQR THE PROSIT METHOD CAN GIVE ANY STATISTICALLY SOUND RESULTS.
I/>Ch CQ'" Test Organism;;g, costatum.I M, bahia M.menidia A, tonsa ,..~I>~~Hours~/LC50(jp~)~EC50'.Phase;liquid suspended particulate CLTENT: xPJ/~5J~Ci')20K 30K 40K BOX 60K 70K Percent Dead or Affected 10 BOX 90K 100