ML13289A206

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Sequoyah Nuclear Plant - Annual Water Withdrawal Updates for 2009
ML13289A206
Person / Time
Site: Sequoyah  Tennessee Valley Authority icon.png
Issue date: 02/13/2009
From: Howard S A
Tennessee Valley Authority
To:
Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
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{{#Wiki_filter:S58 090213 801-NPDES CORRESPONDENCE February 1.3, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JANUARY 2009 Enclosed is the January 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, BR 4T-C A. A. Ray, WT 7C-K B. A. Wetzel, OPS 4A-SQN G. R. Signer, WT 6A-K EDMS, WT CA-K (Enclosure) DMR0901.doc REVIEW/CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-JANUARY DMR ORGANIZATION: Chemistry and Environmental DOCUMENTPREPAREDBY: AnnHurt DATE: 02/11/2009 EDMS TRACKING NO.: S58 090213 801 -NPDES Correspondence Name Ann Hurt Stephanie A Howard Alan K Barringer Debra J Bodine Beth A. Wetzel Chris R Church Timothy P Cleary Stephanie A. Howard (for signature) CONCURRENCES R c Signature -Comment v N X X X X X X X X Date 2-/t3/0 1 INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A /0/) review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 February 13, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORINGREPORT FOR JANUARY 2009 Enclosed is the January 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant. Piease contact me at (423) 843-6700 if you have any questions or comments. Sincerely, 1 J J I+* *1cuv .. L fJ . (/._Ji?lt.c)tLZ. c /4111Jf"i Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res§_ 20QQ_ ___________ _ ______ _ Facility_ JYA -.§EQUOYAH NUCLEAR PLANT _____ _ Locatio.!!_ .J:!AMIL TOl:!_COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMRI TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT Form Approved OMB No. 2040-0004 ATTN: Stephanie A. Howard YEAR I MO DAY I DAY From I 09 01 01 To 31 NO DISCHARGE [ I ... NOTE: Read instructions before completinq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM WATERDEG.

                • ******** ******** ******** 10.6 0 . DIFF. BETWEEN SAMP. & UPSTRM DEG.C 16 1 w 0 EFFLUENT GROSS VALUE PH 00400 1 0 0 EFFLUENT GROSS VALUE I:SULIU:S.

TOTAL SUSPENDED I 50050 0 0 0 0 GROSS VALUE 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Site Vice President TYPED OR PRINTED .. I Certify under penalty of law thai this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, includ1ng the possibility of fine and impnsonment for knowing violations. COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) a.dbJA.)(_Lcd Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT No closed mode operation. The following information is included in an attachment:

1. CCW data 2. veliger monitoring data EPA Form 3320-1 (REV 3/99) Previous editions may be used NO. IFREOUENCYI SAMPLE EX OF TYPE ANALYSIS 04 0 31 I 31 I MODELD TELEPHONE DATE I 1 I I 423 843-6700 09 02 I 11 I I l ' AREA I MO I DAY1 CODE Page 1 of 2 DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 01/14/2009

@2 1120 0.17mg/l 01/15/2009@ 1918 KLM TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 01/14/2009 @ 1117 <0.10 mg/1 01/15/2009@ 1928 KLM TN EPA 8015 Mean# of Water Temp. Mean# of Water Temp. LOCATION SUB NOTES:% Sample Date ZM/m3 % Settlers ("C) Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY Clams/m3 ("C) Clam 1112112008 0 0 12 1111612008 230 12 In plant RCW Dick Adcock 1112412008 31 0 11 1112412008 138 11 In plant RCW Dick Adcock 121412008 0 0 10 1210412008 15 10 In plant RCW Dick Adcock 121812008 0 0 9 1210812008 0 9 In plant RCW Dick Adcock 1211512008 0 0 9 1211512008 0 9 lnplant RCW Dick Adcock 1212212008 0 0 9 1212212008 0 9 In plant RCW Dick Adcock 1212912008 0 0 9 1212912008 0 9 In plant RCW Dick Adcock 0110912008 0 0 8 0110912009 0 8 In plant RCW Dick Adcock 0111612008 0 0 7 0111612009 0 7 In plant RCW Adcock I Bryant 01122/2008 0 0 7 01/2212009 0 7 In plant RCW Adcock I Bryant 01/3012009 0 0 9 0113012008 0 9 lnplant RCW Adcock I Bryant PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L ...f.SL_BOX 2000 ___________ _ -__ 20DD_!:_-DAISY _ _JN 37381._ ______ _ Facilitv _ _IYA -..§!:QUOYAH NUCLEAR PLANT _____ _ Locatio.!l_ TOJi.COUNTL _________ _ ATIN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) TN0026450 101 G YEAR From I 09 I --I --I MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE D *** Form Approved. OMS No. 2040-0004 NOTE Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION \ NO. I FREQUENCY\ SAMPLE EX OF I TYPE LORINE, TOTAL RESIDUAL 0 0 -C, RATE OF 0 0 EFFLUENT GROSS VALUE AVERAGE MAXIMUM ******** ******** UNITS MINIMUM AVERAGE MAXIMUM ******** 0.014 0.030 NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my direction or superv1sion in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted IS , to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and Imprisonment for know1ng violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injection occured: 1. Biodetergent 73551 (max. calc. cone. was 0.025mg/L--Iimit 2.0mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used UNITS 19 0 TELEPHONE DATE --423 843-6700 09 I 02 11 I l AREA I NUMBER YEAR( MO DAY CODE Page 2 of 2 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres,L 2000 ___________ _ -__ _§ODD..Y..: DAISY _ ___IN 373BL ______ _ Facilitv_ .JYA-SEQUOYAH NUCLEAR PLANT _____ _ __!:!AMIL TO.!i.COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 101 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR DAY From 09 To 31 MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 BIOMONITORING FOR OUTFALL 101 EFFLUENT NO DISCHARGE CJ *** NOTE Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. I FREQUENCY EX OF SAMPLE TYPE STATRE 7DAY CHR RIODAPHNIA 1 0 0 7DAY CHR 0 0 NAME!TITLE PRINCIPAL EXECUTIVE OFFICER AVERAGE MAXIMUM UNITS I MUM ******** ******** I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete I am aware that there are Significant penalties for submitting false information, including the possibility of fine and Imprisonment for knowtng violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in January 2009. EPA Form 3320-1 (REV 3/99) Previous editions may be used Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT ANALYSIS TELEPHONE i DATE i ! 423 843-6700 I 09 02 11 I I AREA I NUMBER iYEAR MO DAY COOE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres,L ..£ ___________ _ -__ __§_ODD.r_- DAISY _ _JN 373BL ______ _ Facility_ .JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!!_ .J:!AMIL TOli_COUNTy_ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT !DMRJ TN0026450 103 G PERMIT NUMBER DISCHARGE NUMBER YEAR DAY From 09 31 MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 LOW VOL. WASTE TREATMENT POND EFFLUENT NO DISCHARGE D *** NOTE: Read instructions before completinq this form. QUANTITY OR LOADING QUALITY OR CONCENTRATION \ NO. (FREQUENCY\ SAMPLE EX ! OF \ TYPE PARAMETER AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM PH 0 0 IN CONDUIT OR THRU ENT PLANT 1 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Site Vice President TYPED OR PRINTED ******** ******** 7.0 I Certify under penalty of law that this document and all attachments were prepared under my direct1on or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the information, the information submitted IS . to the best of my knowledge and belief, true, accurate, and complete I am aware that there are sigmficant penalties for submitting false information, including the possibility of fine and Imprisonment for knowing violations.

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used ******** 8.4 Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT UNITS 12 0 TELEPHONE DATE 423 843-6700 09 02 11 I AREAl NUMBER YEAR MO DAY CODE I Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

___ _ Addres_L ___________ _ -__ _§_ODD..Y_- DAISY _ _JN 373BL_ ______ _ Facilitv_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio!J_ _!:!AMIL TOl:!_COUNTY._ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 107 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD From I I To MAJOR (SUBR 01) F-FINAL METAL CLEANING WASTE POND EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. \FREQUENCY I SAMPLE EX 1 OF TYPE AVERAGE MAXIMUM UNITS MINIMUM UNITS ANALYSIS ******** ******** ******** ** 12 0 0 TOTAL (AS CU) 01042 1 0 0 EFFLUENT GROSS VALUE IRON, TOTAL (AS FE) 0 0 DUITORTHRU PLANT 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel ! Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or 09 1 persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 02 11 information, the information submitted 1S , to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I

  • I I I I information, Including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR[ MO DAY I TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

___ _ ______ _ Facility_ .JYA -..§E.QUOYAH NUCLEAR PLANT _____ _ Locatio..!l._ _jjAMIL TO.!:!._ COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES} DISCHARGE MONITORING REPORT (OMR) TN0026450 110 G PERMIT NUMBER DISCHARGE NUMBER MONITORJNG PERIOD YEAR I YEAR I MO I DAY From I 09 To 09 01 31 MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form PARAMETER NO. !FREQUENCY! SAMPLE EX OF TYPE QUALITY OR CONCENTRATION QUANTITY OR LOADING 0 0 0 0 GROSS VALUE 0 AVERAGE MAXIMUM ******** ******** UNITS MINIMUM AVERAGE MAXIMUM ******** ******** 04 f NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my & I direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or I I persons who manage the system, or those persons directly responsible for gathenng the mformation, the information submitted IS , to the best of my knowledge and belief, true, Site Vice President accurate, and complete I am aware that there are s1gnific;ant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT UNITS ANALYSIS 04 TELEPHONE I DATE ! i ! 423 843-6700 09 02 11 I AREAl NUMBER YEAR MO DAY* CODE Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved OMB No. 2040-0004 Addres_L ___________ _ -__ _§.ODDY-DAISY _ _IN 37381_ ______ _ Facilitv _ _IVA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio!l._ .J::!AMIL TO.!i._COUNTY __________ _ ATTN: Stephanie A Howard PARAMETER 0 EFFLUENT GROSS VALUE TN0026450 From QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM ******** ******** RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** NOTE: Read instructions before complettn!l this form QUALITY oR coNCENTRATION (No. 1 FREouENcvl I EX I OF , TYPE AVERAGE MAXIMUM UNITS ANALYSIS ******** ******** 23 ANIIIUAL NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my ,JitphaJt,<L Q.JkKXud I TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualif1ed personnel i Timothy P. Cleary properly gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are Significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 843-6700 09 I 02 11 I AREA I NUMBER I YEAR I MO I DAY I CODE i i Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ ___ _§.ODD_Y._- DAISY _ _TN 3738i_ ______ _ Facility_ JYA -_g:QUOYAH NUCLEAR PLANT _____ _ LocatiO!!... _t!AMIL TO..!'i._COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRI TN0026450 ! *-*-116_6_l YEAR DAY From I 09 I --I --I 31 MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT NO DISCHARGE D ... Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completin>J this form. QUALITY OR CONCENTRATION I NO. I FREQUENCY EX OF PARAMETER SAMPLE TYPE IUI=tsKI::I, FLOATING (SEVERITY) 0 0 ENT GROSS VALUE I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my I direction or superv1sion in accordance with a system designed to assure that qualified personnel I 0 0 TELEPHONE ANALYSIS 1 I 31 SEE PERMIT 1 I 31 l I VISUAL VISUAL VISUAL DATE I I ) 0 .cllnco./cd I I Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or I persons who manage the system, or those persons directly responsible for gathenng the I 09 1 11 I I information, the information submitted is , to the best of my knowledge and belief, true, Principal Environmental Engineer 423 843-6700 02 Site Vice President accurate, and complete. I am aware that there are Significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I i information, including the possibility of fine and Imprisonment for knowing violat1ons. OFFICER OR AUTHORIZED AGENT AREAl NUMBER jYEARj MO DAY J I TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ ___ ______ _ Facilitv_ .JYA -..§!:QUOYAH NUCLEAR PLANT _____ _ Locatio!)_ .J:!AMIL TOJi.COUNTY._ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) TN0026450 117 G F -FINAL PERMIT NUMBER DISCHARGE NUMBER BACKWASH __MQJ\jl_TORING PERIOD EFFLUENT From NO DISCHARGE CJ ... Form Approved. OMB No. 2040-0004 ATIN: Stephanie A. Howard j I To c;1Y NOTE: Read instructions before completinq th1s form PARAMETER QUALITY OR CONCENTRATION I NO. \FREQUENCY EX OF SAMPLE TYPE MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS RIS, FLOATING (SEVERITY) 0 9A 0 1 I 31 I VISUAL ******** ******** 0 SEE VISUAL PERMIT IL AND GREASE VISUAL 1 I 31 VISUAL I 0 0 SEE I VISUAL PERMIT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE I DATE direction or supervision in accordance with a system designed to assure that qualified personnel I I Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or I persons who manage the system, or those persons directly respons1ble for gathering the Principal Environmental Engineer 423 843-6700 o9 1 02 11 information, the information submitted is . to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are S1gnif1cant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I i informat1on, Including the possibility of fine and imprisonment for know1ng violations. OFFICER OR AUTHORIZED AGENT AREAl NUMBER MO DAY TYPED OR PRINTED CODE -------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Addres_L ___________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT !DMR) TN0026450 118 G MAJOR (SUBR 01) F-FINAL Form Approved OMB No 2040-0004 ______ _ PERMIT NUMBER DISCHARGE NUMBER I WASTEWATER & STORM WATER Facility_ JYA -..§E:QUOYAH NUCLEAR PLANT _____ _ Locatio!!_ _jjAMIL TOJi_COUNTY __________ _ EFFLUENT MONITORING PERIOD ATTN: Stephanie A. Howard From NOTE: Read instructions before completinq this form. YEAR DAY 09 To 31 NO DISCHARGE IXXJ *** PARAMETER QUALITY OR CONCENTRATION I NO. I FREQUENCY 'I SAMPLE EX OF TYPE (DO) DUIT ORTHRU T PLANT 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER UNITS MINIMUM AVERAGE ******** I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel MAXIMUM UNITS ******** 19 MG/L 19 TELEPHONE ANALYSIS TWICE/ WEEK GRAB GRAB ESTIMA DATE I U I I Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or 11 1 persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 I og 02 Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, I I accurate, and complete. I am aware that there are significant penallies for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT l NUMBER I YEAR MO I DAY I TYPED OR PRINTED I I I J COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 S58 090312 800-NPDES CORRESPONDENCE March 12, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR FEBRUARY 2009 Enclosed is the February 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely, Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, BR 4T-C A. A. Ray, WT 7C-K B. A. Wetzel, OPS 4A-SQN G. R. Signer, WT 6A-K EDMS, WT CA-K (Enclosure) DMR0902.doc REVIEW/CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-FEBRUARY DMR ORGANIZATION: Chemistry and Environmental DOCUMENTPREPAREDBY: AnnHurt DATE: 03/09/2009 EDMS TRACKING NO.: S58 090312 800-NPDES Correspondence CONCURRENCES Name R c Signature -Comment Date v N Ann Hurt X ar Stephanie A Howard X 3}q fo9 Alan K Barringer Debra J Bodine Beth A. Wetzel Chris R Church X Timothy P Cleary X (for signature) INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 March 12, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR FEBRUARY 2009 Enclosed is the February 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely, Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 i PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Add res.§_ ___________ _ -__ _§_ODDY-DAISY _ _TN 3738L ______ _ Facility_ .JYA -.§E:QUOYAH NUCLEAR PLANT _____ _ LocatioD__ __!jAMIL TOJ::LCOUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER TEMPERATURE, WATER DEG_ CENTIGRADE 00010 z 0 0 INSTREAM MONITORING TEMPERATURE, WATER DEG. CENTIGRADE 00010 0 0 EFFLUENT GROSS VALUE TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG_C 00016 1 w 0 EFFLUENT GROSS VALUE PH 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 00556 0 0 EFFLUENT GROSS VALUE I FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 0 EFFLUENT GROSS VALUE TN0026450 PERMIT NUMBER YEAR From 09 QUANTITY OR LOADING AVERAGE ******** MAXIMUM ******** . ' UNITS MGD 101 G DISCHARGE NUMBER DIFFUSER DISCHARGE EFFLUENT *** NO DISCHARGE [J *** DAY 28 NOTE: Read instructions before completinq this form. MINIMUM QUALITY OR CONCENTRATION I NO.IFREOUENCYj SAMPLE EX OF I TYPE AVERAGE MAXIMUM UNITS ANALYSIS ' ******** . I DEG. C. I .'OgllY -MX . ******-*.. I . 6 I 04 I 0 I 28/ 28 I MOOELD .............. . . I SEE PERMIT 25.9 04 0 28 I 28 I RCORDR : I i ... DEG.C. SEE I CK REO. PERMIT ******** ******** 3.3 <6 .* F.

                • 04 su 19 MG/L 19 MG/L o 1 28t 28 CALCTD j . CONTIN CALCTD uous 4 I 28 I GRAB I I GRAB< 0 I 1 /28 ] GRAB I MONTHL Yl GRAB /28 I GRAB V'IGNTHI;:.YI GRAB 0 1:: u uous NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that.this doc.ument and a. II attachments w.ere prepare.d under my
  • Q 1./,.._ , ....,_ * / TELEPHONE I D.ATE direction or supervision in .accordance with a system designed to assure that qualified personnel
  • u,ur l,.c._.)C..LA c::1 , 1 Timothy P. Cleary properly gather and evaluate the 1nformahon subm1tted Based on my *nqu1ry of the person or ! 1 persons who manage the system,or those persons respons*ble for gathenng the Principal Environmental Engineer 23 8 3 6 OO 09 1 03 1 1 10 . . . information.

the mlormation submitted 1s . to the best of my knowledge and belief, true, 4 4 -7 l' I Sequoyah Stte Vtce President accurate, and complete. 1 am aware that there are significant penalties lor submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I f----------------------l'nformatJon, including the possibility of fine and imprisonment for know1ng violalJans OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR I MO 1 DAY TYPED OR PRINTED CODE i COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment:

1. CCW data 2. Veliger monitoring data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2 DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 02/11/2009@

1058 0.13 mg/1 02/13/2009 @ 1136 DAP TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 02/11/2009 @ 1100 <0.11 mg/1 02/13/2009 @ 1146 DAP TN EPA 8015 Mean# of Water Temp. Mean# of Water Temp. LOCATION SUB NOTES:% Sample Date % Settlers {"C) Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Clams/m3 ("C) Clam 1112112008 0 0 12 1111612008 230 12 In plant RCW Dick Adcock 1112412008 31 0 11 1112412008 138 11 lnplant RCW Dick Adcock 121412008 0 0 10 1210412008 15 10 In plant RCW Dick Adcock 121812008 0 0 9 1210812008 0 9 lnplant RCW Dick Adcock 1211512008 0 0 9 1211512008 0 9 In plant RCW Dick Adcock 1212212008 0 0 9 1212212008 0 9 In plant RCW Dick Adcock 12129/2008 0 0 9 12129/2008 0 9 lnplant RCW Dick Adcock 0110912009 0 0 8 0110912009 0 8 In plant RCW Dick Adcock 0111612009 0 0 7 0111612009 0 7 In plant RCW Adcock I Bryant 0112212009 0 0 7 0112212009 0 7 In plant RCW Adcock I Bryant 0113012009 0 0 9 0113012009 0 9 In plant RCW Adcock I Bryant 0210612009 0 0 6 0210612009 0 6 lnplant RCW Adcock I Bryant 0211312009 0 0 11 02/1312009 0 11 In plant RCW Love 0212012009 0 0 9 0212012009 0 9 In plant RCW Bryant 0212612009 0 0 9 0212612009 0 9 In plant RCW Bryant PERMIITEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Add res§_ 2000 ___________ _ -__ __§.ODDY-DAISY _ _JN 37381._ ______ _ Facility_ ..JYA -..§!:QUOYAH NUCLEAR PLANT _____ _ Locatio.!l.._ _!:!AMIL TOJi.COUNTY_;_ _________ _ ATIN: Stephanie A Howard PARAMETER NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) Form Approved OMS No. 2040-0004 TN0026450 101 G F-FINAL Dl FFU SER DISCHARGE PERMIT NUMBER MONITORING PERIOD EFFLUENT *** NO DISCHARGE [J *** YEAR DAY From I 09 To 28 NOTE: Read instructions before completinq this form. QUALITY OR CONCENTRATION I NO. )FREOUENCYTSAMPLE-EX i OF : TYPE ANALYSIS _ __ MAXIMUM UNITS MINIMUM AVERAGE . . . ----+ ---+----_; ________ j CHLORINE, TOTAL RESIDUAL ******** ********

                • 0.016 0.030 I 19 i 0 1 19 I 28 : GRAB . L_ 0 0 RE-C, RATE OF 82234 0 0 EFFLUENT GROSS VALUE I Certify under penalty of law that this document and all attachments were prepared under my d NAME/TITLE PRINCIPAL EXECUTIVE OFFICER direction or supervision in accordance with a system designed to assure lhat qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE informatton. includtng the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injection occured: Biodetergent 73551 (max. calc. cone. was 0.021 mgiL--Iimit 2.0mgiL) EPA Form 3320-1 (REV 3/99) Previous editions may be used MG/L 0 TELEPHONE 423 843-6700 I AREAl NUMBER CODE WEEK-I CALCTD DAYS 28 I 28 I CALCTD I DATE I , i 1 09 03 I i 10 J [YEAR 1 MO j DAY I Page 2 of 2 PERMITIEE NAME!ApDRESS (Include Facilitv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) Form Approved OMB No. 2040-0004 ___ _§ODDY-DAISY _ _JN 37381._ ______ _ Facility _ _IVA -SEQUOYAH NUCLEAR PLANT _____ _ Locatio.n_ .JjAMIL TOJLCOUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER TN0026450 From QUANTITY OR LOADING AVERAGE ******** ******** >100.0 F-FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT NO DISCHARGE CJ *** NOTE: Read instructions before completinQ this form. QUALITY OR CONCENTRATION I NO. IFREO EX OF ******** ******** 23 0 PERCENT 23 0 ANALYSIS 1 I 180 SEE PERMIT COMPOS 1 f 180 i COMPOS _J f I I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed lo assure thai qualified personnel 091 ! Timothy P. Cleary properly gather and evaluate lhe information submilled. Based on my inquiry of lhe person or 1 10 persons who manage lhe system, or those persons directly responsible for gathering the information, the information submitted is , to lhe best of my knowledge and belief. lrue, I Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, lhe possibility of ftne and imprisonment for knowing violations I --TYPED OR PRINTED --------*-COMMENTS AND EXP,LANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled February 8-13, 2009. Report is attached EPA Form 3320-1 (REV 3199) Previous editions may be used ' Principal Environmental Engineer 423 843-6700 03 SIGNATURE OF PRINCIPAL EXECUTIVE l i OFFICER OR AUTHORIZED AGENT AREA 1 NUMBER YEAR[ MO I DAY i CODE I : ! Page 1 of 1 March 9, 2009 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, FEBRUARY, 2009 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101, samples collected February 8-13, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values for both species were> 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment): Sherrard, R. M., PSC 1X-C Files, ER&TA, CEB IB-M SQN February 2009m TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: March 9, 2009 1. Facility I Discharger: Sequoyah Nuclear Plant I TV A 2. County I State: Hamilton I Tennessee

3. NPDES Permit#: TN0026450
4. Type of Facility:

Nuclear-Fueled Electric Generating Plant 5. Design Flow (MOD): 1,579 6. Receiving Stream: Tennessee River (TRM 483.6) 7. 1Q10: 3,491 8. Outfall Tested: 101 9. Dates Sampled: February 8-13, 2009 10. Average Flow on Days Sampled (MOD): 1582, 1597, 1596 11. Pertinent Site Conditions: Production I operation data will be provided upon request. 12. Test Dates: February 10-17, 2009 13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia) 15. Concentrations Tested(%): Outfall101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephales promelas: UV treated Outfall101: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0 16. Permit Limit Endpoint (% ): Outfall 101: ICzs = 45.2% 17. Test Results: OutfalllOl: Pimephales promelas: IC 25 > 100% Ceriodaphnia dubia: IC2s > 100% UV treated Outfall101: Pimephales promelas: IC£2 > 100% Page 1 of98

18. Facility Contact: Ann Hurt Phone#: (423) 843-6714 19. Consulting I Testing Lab: Environmental Testing Solutions, Inc. 20. Lab Contact: Jim Sumner Phone #: (828) 350-9364 21. TVA Contact: Cynthia L. Russell Phone#: (256) 386-2755 22. Notes: OutfalllOl samples collected February 8-13, 2009, showed no toxic effects to fathead minnows or daphnids.

The resulting IC 25 values, for both species, were> 1 00 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Page 2 of98 Fathead minnows were also exposed to UV treated Outfall 1 01 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. METHODS

SUMMARY

Samples: 1 . Sampling Point: Outfall 1 0 1, Intake 2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. t TRC* Time (ET) ID Collected Received (OC) (mg/L) Last Used By 101 02-08-09 0921 to 02-09-09 1554 1.7, 1.9 <0.10 02-10-09 1110 02-09-09 0821 02-11-09 1206 Intake 02-08-09 0940 to 02-09-09 1554 2.4 <0.10 02-10-09 1110 02-09-09 0840 02-11-09 1206 101 02-10-09 0915 to 02-11-09 1553 2.2, 1.6 <0.10 02-12-09 1 030 02-11-09 0815 02-13-09 1110 Intake 02-10-09 0936 to 02-11-09 1553 3.1 <0.10 02-12-09 1030 02-11-09 0836 02-13-09 1110 02-12-09 0936 to 02-14-09 1012 101 02-13-09 0836 02-13-09 1531 1.5, 1.5 <0.10 02-15-09 1014 02-16-09 1011 02-12-09 0842 to 02-14-09 1012 Intake 02-13-09 1531 0.7 <0.10 02-15-09 1014 02-13-09 0742 02-16-09 1011 *TRC =Total Residual Chlonne tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival. 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath. Page 3 of98 Aliquots of Outfall 101 and Intake samples were UV -treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Pimephales promelas Test Organisms:

1. Source: Aquatox, Inc. 2. Age: 19 to 19.17 hours old Test Method Summary: 1. Test Conditions:

Static, Renewal 2. Test Duration: 7 days 3. Control I Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

1 5. Organisms per Replicate: 1.Q 6. Test Initiation: (Date/Time) Outfall101 02-10-09 1110 ET UV Treated Outfall 101 02-10-09 1100 ET 7. Test Termination: (Date/Time) Outfall 101 02-17-09 1030 ET UV Treated Outfall 1 01 02-17-09 1 046 ET 8. Test Temperature: Outfall 101: Mean= 24.6°C (24.1-24.9°C} Ceriodaphnia duhia In-house Cultures <24-hours old Static, Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic 1 02-10-09 0830 ET 02-16-09 0913 ET Mean= 24.8°C (24.5-25.1 °C) Test Temperature: UV-Treated Outfall101: Mean= 24.6°C (24.2-25.1 °C} 9. Physical I Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and exposure test solutions were analyzed daily for pH and dissolved oxygen. 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyyille, CA). Page 4 of98 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1. Results of a Pimephales pro mel as Chronic/ 7 -day Toxicity Test. (Genus species) (Type I Duration) Conducted February 1 0 -17, 2009 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used-days) (%Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 98 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 98 Test Solutions Mean Dry Weight (mg) (% Effluent) (replicate number) 1 2 3 4 Mean Control 0.644 0.678 0.597 0.563 0.621 11.3% 0.630 0.628 0.616 0.683 0.639 22.6% 0.670 0.679 0.612 0.743 0.676 45.2% 0.614 0.619 0.693 0.525 0.613 72.6% 0.577 0.522 0.658 0.579 0.584 100.0% 0.605 0.742 0.593 0.534 0.619 Intake 0.622 0.612 0.599 0.553 0.597 ICzs Value: > 100% Calculated TU Estimates: < 1.0TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso: TUc = 100/ ICzs Page 5 of98 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubio Chronic/ 7 -day Toxicity Test. (Genus species) (Type I Duration) Conducted February 10-16, 2009 using effluent from Outfall 101. Percent Surviving Test (time interval used-days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/6 days) (% Effluent) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 34 31 31 30 34 35 29 31 33 32 32.0 11.3% 33 33 35 31 35 34 30 32 31 . 32 32.6 22.6% 35 31 33 32 35 35 31 35 33 35 33.5 45.2% 35 35 33 32 36 35 35 33 35 33 34.2 72.6% 35 34 37 34 35 38 36 37 36 34 35.6 100.0% 37 34 39 38 36 39 36 35 39 36 36.9 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Pennit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso: TUc = 100/ ICzs Page 6 of98 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/7-day Toxicity Test. (Genus species) (Type I Duration) Conducted February 10-16, 2009 using water from Intake Percent Surviving Test (time interval used-days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/6 days) (% Effluent) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 32 31 31 33 33 31 33 32 31 30 31.7 Intake 34 37 35 33 37 40 35 34 37 35 35.7 IC2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/ A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LC 5 o: TUc = 100/ IC2s Page 7 of98 TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets) 3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted February 10-17,2009 using effluent from UV Treated Outfall101. Test Percent Surviving Solutions (time interval used-days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg) (% Effluent) (replicate number) 1 2 3 4 Mean Control 0.611 0.630 0.610 0.665 0.629 11.3% 0.642 0.720 0.729 0.747 0.710 22.6% 0.664 0.740 0.636 0.663 0.676 45.2% 0.716 0.622 0.516 0.577 0.608 72.6% 0.642 0.571 0.639 0.676 0.632 100.0% 0.626 0.654 0.647 0.688 0.654 Intake 0.567 0.601 0.586 0.717 0.618 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso: TUc = 100/ IC2s REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) Species Date Time Duration Toxicant Results (IC2s) Pimephales promelas February 10-17, 2009 1130 7-days KCl 0.83 giL Ceriodaphnia dubia February 3-9, 2009 0750 6-days NaCI 1.05 g/L Page 8 of98 PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pimepholes promelos and Ceriodophnio dubio Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfull 101 performed February I 0-17, 2009. Test Sample ID Temperature (0 C) Initial Control 24.7 24.6 -24.8 11.3% 24.7 ,., 24.7 -24.9 22.6% 24.8 !; "' 24.7 -24.9 .... 24.8 'I:>.. 45.2% 24.7 -24.9 "' -I: 24.8 72.6% 24.7 -24.9 100.0% 24.8 24.7 -24.9 Intake 24.8 24.7 -24.9 Control 24.7 24.5 -24.8 11.3% 24.8 24.7 -25.0 .:! 24.8 ""' 22.6% .§ 24.7 -25.0 -:! 24.8 ::: 45.2% -I: 24.7 -25.0 "' 72.6% 24.8 *;:: 24.7 -25.0 " \,) 24.9 100.0% 24.7 -25.1 Intake 24.7 24.6 -24.9 ---**---Overall temperature ('C) Pimepholes promelos Ceriodophnio dubio Page 9 of98 Final 24.5 24.2 -24.7 24.5 24.1 -24.7 24.5 24.3 -24.7 24.4 24.2 -24.5 24.4 24.2 -24.6 24.4 24.2 -24.6 24.4 24.2 -24.5 24.8 24.6 -25.0 24.8 24.6 -24.9 24.9 24.8 -25.0 24.8 24.7 -25.1 24.8 24.7 -24.9 24.8 24.6 -24.9 24.8 24.6 -25.0 Average 24.6 24.8 Dissolved Oxygen (mg/L) Initial Final 7.7 7.4 7.5 8.2 6.6 7.7 7.9 7.4 7.7 8.4 6.6 -7.8 7.9 7.4 7.7 -8.4 6.2 -7.8 7.9 7.4 7.7 -8.5 6.3 7.7 8.0 7.4 7.7 -8.5 6.3 -7.7 8.0 7.3 7.7 -8.5 6.3 7.7 8.0 7.4 7.7 -8.5 6.4 7.7 7.7 7.8 7.5 8.2 7.5 8.2 7.9 7.8 7.7 -8.4 7.6 -8.2 7.9 7.8 7.7 -8.4 7.6 8.2 8.0 7.8 7.7 -8.5 7.6 8.2 8.1 7.7 7.8 8.5 7.5 8.2 8.0 7.7 7.7 -8.5 7.5 -8.0 8.1 7.7 7.7 -8.5 7.6 -8.1 Minimum Maximum 24.1 24.9 24.5 25.1 pH (S.U.) Conductance Alkalinity Hardness Total Residual ; initial Final (f.tmhos/cm) (mg/L (mg/L CaC0 3) Chlorine (mg/L) I 7.78 7.69 324 63 95 -7.69 -8.01 7.44 -7.81 306 345 62 -64 92 98

  • 7.85 7.66 310 ---7.80 -7.88 7.30 -7.80 300 332 -7.86 7.67 292 ---7.81 -7.89 7.38 -7.79 282 300 ---7.87 7.67 269 ---7.81 -7.90 7.38 -7.81 262 -275 ----7.88 7.70 240 ---7.81 -7.92 7.39 -7.83 233 -245 ---7.89 7.71 211 73 85 < 0.10 7.81 -7.94 7.40 -7.83 204 -218 70 -75 82 86 < 0.10 -<0.10 7.90 7.71 211 73 86 < 0.10 7.85 -7.95 7.40 -7.84 206 -218 72 -74 82 92 < 0.10 -<0.10 7.79 7.81 321 63 95 -7.69 -8.01 7.75 -7.89 306 325 62 -64 92 98 7.85 7.80 309 ---7.80 -7.88 7.75 -7.88 300 332 ---7.87 7.80 291 ---7.81 -7.89 7.74 -7.86 282 300 --7.88 7.82 268 ---7.81 -7.90 7.75 -7.87 262 -27 5 -7.89 7.82 239 ---7.81 -7.92 7.75 -7.87 233 245 ---7.90 7.84 211 73 85 < 0.10 7.81 -7.94 7.76 -7.89 204 -218 70 75 82 86 < 0.10 -<0.10 7.91 7.85 210 73 86 < 0.10 7.85 -7.95 7.76 -7.90 206 217 72 74 82 92 < 0.10 -<0.10 PHYSICAL/CHEMICAL

SUMMARY

Water Chemistty Mean Values aud Ranges for Pimephales promelas Tests, UV-treated Sequoyah Nuclear Plaut (SQN) Outfall 101 performed February 10-17, 2009. Test Sample lD Temperature ("C) Initial Final Control 24.7 24.6 24.6 -24.8 24.5 -24.6 24.8 24.5 11.3% 24.7 -24.9 24.3 -24.6 "' 22.6% 24.8 24.4 £:: 24.7 -25.0 24.2 -24.7 c .. 24.8 24.4 45.2% -24.7 -25.0 24.2 -24.7 24.8 24.4 72.6% .§ 24.7 -25.0 24.2 -24.7 24.9 24.4 100.0% 24.7 -25.1 24.2 -24.6 Intake 24.9 24.5 24.7 -25.0 24.2 -24.7 Overall temperature ("C) Pimephales promelas Average 24.6 Page 10 of98 Dissolved Oxygen (mg/L) Initial 7.9 7.6 -8.5 7.9 7.6 -8.5 7.9 7.6 -8.5 7.9 7.7 -8.5 8.0 7.7 -8.5 8.0 7.7 -8.4 8.0 7.8 -8.7 __ Minimum 24.2 Final 7.4 6.7 -7.8 7.3 6.6 -7.6 7.3 6.5 -7.8 7.4 6.5 -7.8 7.3 6.4 -7.7 74 6.4 -7.7 7.2 6.4 -7.4 Maximum 25.1 pH (S.U.) Conductance Alkalinity Initial Final (1-1mhos/cm) (mg/L CaC0 3) 7.83 7.54 310 62 7.74 -7.91 7.06 -7.73 303 -318 61 -63 7.76 7.54 304 -7.15 -7.91 7.04 -7.76 296 -313 --7.76 7.55 293 -7.15 -7.91 7.04 -7.75 287 -303 --7.77 7.57 271 -7.15 -7.91 703 -7.74 263 -279 --7.77 7.58 239 -7.15 -7.92 7.02 -7.78 234 -245 --7.78 7.59 211 71 7.14 -7.93 7.02 -7.77 204 -218 70 -72 7.75 7.58 209 72 7.14 -7.92 _6.99 -7.83 197 -216 69 ---* . -Hardness (mg/L CaC0 3) 91 90 -----------85 t\4 -86 85 84 -86

SUMMARY

I CONCLUSIONS Page 11 of 98 Outfall101 samples collected February 8-13,2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Appendix A ADDITIONALTOXICITY TEST INFORMATION

SUMMARY

OF METHODS 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment.

Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution. DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL 1. Pimephales promelas None 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None 2. Ceriodaphnia dubia None Page 12 of98 PHYSICAL AND CHEMICAL METHODS 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
2. Instruments:

All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B. 4. Dissolved oxygen was measured by SM 4500 0 G. 5. The pH was measured by SM 4500 H+ B. 6. Conductance was measured by SM 2510 B. 7. Alkalinity was measured by SM 2320 B. 8. Total hardness was measured by SM 2340 C. 9. Total residual chlorine was measured by ORION Electrode Method 97-70. QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013.

Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7 -day chronic tests with results expressed as IC 25 values in giL KCl or N aCl. 2. Standard Toxicant:

Potassium Chloride (KCl crystalline) for Pimephales promelas. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia. 3. Dilution Water Used: Moderately hard synthetic water. 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses. Page 13 of 98 REFERENCES

1. NPDES Permit No. TN0026450.
2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002). 3. Standard Methods for the Examination of Water and Wastewater, 20th Edition, 1998. \ 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 14 of98 Page 15 of98 Sequoyah Nuclear Plant Biomonitoring February 10-17, 2009 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 16 of98 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998-February 13,2009 PCL-401 mg/L Copolymer 0.005 0.011 0.021 0.019 0.015 0.015 0.015 0.024 0.024 0.024 0.024 0.024 0.024 0.023 Page 17 of98 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, Date 01/31/2000 02/01/2000 02/02/2000 02/03/2000 02/04/2000 02/05/2000 02/06/2000 12/11/2000 12/12/2000 12/13/2000 12/14/2000 12115/2000 12/16/2000 12117/2000 08/26/2001 08/27/2001 08/28/2001 08/29/2001 08/30/2001 08/31/2001 11/25/2001 11/26/2001 11/27/2001 11/28/2001 11/29/2001 11130/2001 12/09/2001 12/10/2001 12/11/2001 12/12/2001 12/13/2001 12/14/2001 March 12, 1998-February 13, 2009 PCL-401 mg/L Copolymer 0.009 0.028 0.009 0.009 0.009 0.009 0.009 0.019 0.019 0.018 0.019 0.019 0.019 0.019 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.021 0.021 0.021 0.020 0.021 0.020 0.02 0.019 0.019 0.02 0.02 0.02 0.02 0.02 Page 18 of98 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, Date 01/02/2002 01/03/2002 01/04/2002 01/05/2002 01/06/2002 01107/2002 02/24/2002 02/25/2002 02/26/2002 02/27/2002 02/28/2002 03/01/2002 . 05/05/2002 ...* 05/06/2002 05/07/2002 . 05/08/2002 05/09/2002 05/10/2002 08/04/2002 08/05/2002 08/06/2002 08/07/2002 08/08/2002 08/09/2002 10/06/2002 10/07/2002 10/08/2002 10/09/2002 10/10/2002 1 0/11/2 002 F:***:*:***:*:*:*:*:***:********* 01112/2003 01/13/2003 01/14/2003 01/15/2003 01/16/2003 01/17/2003 04/06/2003 04/07/2003 04/08/2003 04/09/2003 04/10/2003 04/11/2003 March 12, 1998 -February 13, 2009 PCL-401 mg/L Copolymer 0.02 0.014 0.014 0.014 0.023 0.023 0.023 0.008 0.008 0.02 0.02 0.019 0.02 0.019 0.018 0.018 0.019 0.019 0.018 0.018 0.018 0.018 0.018 0.018 0.019 0.020 0.020 0.020 0.009 0.021 0.021 0.021 0.021 0.022 Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, Date 06/15/2003 06/16/2003 06/17/2003 .. 06/18/2003 06/19/2003 06/20/2003 08/03/2003 08/04/2003 08/05/2003 08/06/2003

08/07/2003 08/08/2003 10/05/2003 10/06/2003 10/07/2003 10/08/2003 10/09/2003 10/10/2003 02/01/2004 02/02/2004 02/03/2004 02/04/2004 02/05/2004 02/06/2004 05/04/2004 05/05/2004 05/06/2004 05/07/2004 . 05/08/2004 05/09/2004 07/04/2004 07/05/2004 07/06/2004 07/07/2004 07/08/2004 07/09/2004 Page 19 of 98 . March 12, 1998-February 13,2009 PCL-401 mg/L Copolymer 0.020 0.014 0.014 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.022 0.024 0.009 0.009 0.009 0.009 0.009 0.009 0.019 0.014 0.013 0.020 0.021 0.020 0.019 0.020 0.020 0.019 0.019 0.020 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 1 01) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, Date 11/07/2004 11108/2004 11109/2004 11110/2004

.. 11111/2004 11/12/2004 02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/11/2005 06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10/2005 07/17/2005 07/18/2005 07/19/2005 07/20/2005

07/2112005 07/22/2005 10/30/2005 10/31/2005 11/01/2005 11/02/2005 11/03/2005 11/04/2005 11/14/2005

  • .. 11/15/2005 11/16/2005 11117/2005

11/18/2005 11/19/2005 Page 20 of98 March 12,1998 -February 13,2009 Date 11/12/2006 11/13/2006 11114/2006 11/15/2006 11116/2006 11117/2006 11126/2006 11/27/2006 11128/2006 11/29/2006 11130/2006 12/01/2006 05/28/07 05/29/07 05/30/07 05/31/07 06/01/07 06/02/07 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/13/08 04/14/08 04/15/08 04/16/08 04/17/08 04/18/08 10/26/08 10/27/08 10/28/08 10/29/08 10/30/08 10/31/08 Page 21 of98 Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-February 13,2009 Cuprostat-PF mg/L Azole 0.015 0.015 0.015 O.D15 0.015 0.015 0.030 0.030 0.030 Date 02/08/09 02/09/09 02/10/09 02/11/09 02/12/09 02/13/09 Page 22 of98 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 1 01) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Conosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -February 13,2009 Cuprostat-PF mg/L Azole Page 23 of 98 Sequoyah Nuclear Plant Biomonitoring February 10-17, 2009 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets '"C 1.10 tt> s BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 ofl Client: TV A Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery /J Facility Sampled: Sequoyah NP 28801 Geneml Chevy Wtlhamsv . , . .-/ NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell : -..,...,.-,..........-/':.::7 Collected By: Chevy Williams, Ben Mitchell Fax: 828-350-9368 Dissolved Metals Collecte . Samples remained on ice through out sampling and tmnsport to Jab. Field Identification I Grab/Comp. Collection Date/Time Container iJit Sample Description Number& Rain Event? fYWJ Laboratory Use Volume ) (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETSLog Arrival By Time Appear-:c. .jf.' (EST) Inches Number Temp. E\ ance I'< <./ )*"* oc) SQN-101-TOX Comp 02/08/09 -02/09/09 0921-2 (2.5gal) X '\'C.. (\\ \ss'-\

  • Sl4i..ll.Ci 0821 :: \SS"l 1-\<7-t> ,.1 I* SQN-INT-TOX Comp 02/08/09 -02/09/09 0940-1 (2.5 gal) NA X Oill"'Z .L1l-'2.A*c.. >> \Ss*-\ 0840 Sample Custody-Fill In From Top Down <.v.ft ceLt A D ,.jJ A Relinquished By (Signature):

Date/Time Received By (Signature): '-J" '** Ablll.u.JoUI( t..A-.90001. ...... Date/Time d"" 02/09/09 1f: ESl"' Sonic Delivery 02/09/09 E\ ' Sonic Delivelr 02/09/09 ET<;. 02/09/09 ,c;sL\ E\ tSSY Instructjons: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. . ...J)_ I1Q (t> BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 1 ° Client: TV A Environmental Testing Solution, Inc. Delivered By (Circle One): l Project Name: Sequoyah NP Toxicity 3 51 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic . / 1 Facility Sampled: Sequoyah NP 28801 General 1/ fl1 / ./'? Chevy 1'-::f:- /:.___ NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell : *.r-.1

  • Collected By: Chevy Williams, Ben Mitchell Fax: 828-3 5 0-93 68 Dissolved Metals Collected.

Samples remained on ice through out sampling and transport to lab. Field Identification I Grab/Comp. Collection Dateffime Container Flow . 5.2. 4 Sample Description Number & cfs Ram Event? L b t U Volume (Mark as Appropriate) ClU a ora ory se Collected }\fS*;)i;.: < : [J l;,) -:,;/ Date Time ':*\[-,#"::'*: Yes If Yes, No Trace ETS Log Arrival By Time Appear- ,,, :_al_ .-.

. (EST)

Inches Number E\ ance SQN-101-TOX Comp 02/10/09-02/11109 0915-2 (2.5gal) 2464-X 0815 2494 'Z \ .J. AVG=

  • * (j\ \? -'f"' 2471 =

SQN-INT-TOX Comp 02/10/09-02/11109 0936-I (2.5 gal) NA X \*'4

  • i,. 0836 3.\ (_ (} Sample Custody-Fill In From Top Down -Af. -*-ll -_&.

'..,,, ... lid. Relinquished By (Signature): Date/Time Received By (Signature): Dateflime ( fv--...... Chevy Williamy. j 4 / ,f/ 02/11/09 . .* _ . Sonic Delivery 02/ll/09 c_/9//} I I .;<gc5r bIt I;: l<t *\ I Sonic Delivery L) ./ (? .IJ 02/11/09 ETS " I E'TS 02/11/09 1 E"t"" lSS3 ET '-\J 7 Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive Monday. BIOMONITORING CHAIN OF CUSTODY RECORD Page 1oft Client: TV A Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity P.O. Nwnber: N/A Facility Sampled: Sequoyah NP NPDES Nwnber: TN0026450 Collected By: Chevy Williams, Ben Mitchell Field Identification I 1 Grab/Comp. Sample Description 351 Depot Street. FedEx UPS Bus Client Asheville, NC 28801 Phone: 828-350-9364 Fax: 828-350-9368 Collection Date/Time Container I Flow Number& cfs Volume Collected I Time Date (EST) Yes Other (specify): / /! Chevy Williams{ ' * /' . Ben Mitchell =cz Dissolved Metals Collected. Samples remained on ice through out sampling and transport to lab. Rain Event? (Mark as Appropriate) IfYes, I No I Trace Inches e,(1iL 'SZL{(p Laboratory Use ETS Log I Arrival I By I Time I Appear-Number Temp. e:. T ance SQN-101-TOX I Comp o21121o9 -02113109 1 o936-os36 I .. l,t\ I 2 (2.5gal) I 2453-I I I X 2493 AVG= 2470 SQN-INT-TOX I Comp 02/12109 -02113109 1 os42-o742 1 (2.5 gal) I NA I I I X I 0 .1*(.. I 0 J' ,t;,';\ I -If. Sample Custody-Fill In From Top Down

  • A Relinquished By (Signature):

Date/Time Received By (Signature): so .. Date/Time YTJ12Jdvtll I Sonic Delivery 02113/09 /I '!0 ESr fJ N 02/13/09 cJ I(' E\ -----/ Sonic Delive;lg lf2 02/13/09 ETS I ET 1 02/13/09 E.\ Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: TVA Facility: Seguoyab Nuclear Plant NPDES #: TN 0026450 Project #: ____ _ Dilution preparation information: Dilution prep (%) !1.3 22.6 Effluent volume (rnL) 282.5 565 Diluent volume (rnL) 2217.5 1935 Total volume (mL) 2500 2500 Test information: 45.2 72.6 1130 1815 !370 685 2500 2500 Organism age: ,q . 11 l*hu Its o 1..{) Date and times organisms \\coO were born between: 100 2500 0 2500 County: Hamilton Treatment: Non-treated Outfall: 101 Comments: Test information: Randomizing tem.Jl.late: 'I eu.c IQ Incubator number: 3C Pagel of6 Organism source: A--ro"i-. & {J ,P Ol*tl\*cf\ Artemia lot number: Transfer bowl information: pH= Temperature=

  • c Total drying time: 'L"l*

1.* (,1. Date I Time in: o 1* 11*o'\ 1\\D Average transfer volume: o. Date I Time out:tn.*\!*ct\ \\ \D Oven temperature: 'cC"t Daily feeding and renewal information: I I I* I I ' ' Day 0 2 3 4 5 6 7 27 of98 Date Morning Afternoon feeding feeding time time Test initiation, Control water batch used Sample numbers used Analyst ... Page 2 of 6 Species: Pimephales promelas 10 Date: _ _,;.._ Client: TVA I Sequoyah Nuclear Plant* Non-treated Survival and Growth Data Day CONTROL 11.3% 22.6% A B c D E F G H I J K L 0 /t) I{) ID 10 /D 10 to ID 10 ID ID 10 I tb 10 /0 IC IO 10 10 ID tC 10 10 10 2 /D 10 IO l() 10 10 10 lD ID I(J LD ID 3 10 I() ID /D /0 It) I() I() I() 10 /Q 10 4 10 10 10 10 IQ IO 10 10 tO 10 10 10 5 /0 10 ID 10 IO /0 (0 lO IO 10 10 10 6 /D /0 /0 /0 10 /0 /0 to /Q 10 /() 10 7 /0 IO 1\J 10 fC to fD 10 l't; lf:::J 10 /\) A= Pan weight (mg) Tray color code:: Analyst: U1(b 1tf 1"!.1.8 13.1.{1 lt.{.CS t3.1..D lt3.'11 1"\.11 15.'10 t"{.to Date: Cl* \1'\:-CI"' B =Pan + Larvae weight (mg) f"Lr IJZ>*1-b l\.M 11.b8 11.50 IJ.b. i Jb.33 :u.:n I;J.\, ?-1 ,;}.!). ;)b, J.\.}8 Analyst: Date: ol. *o" C = Larvae weight (mg) =A-B l,.t.t"' 1,.18 S.'\, lA* '22 (o.ll.. '-*1b 1.. "" ..... n. Weight per initial number "'" ft.,., ,..,o ""' ,'V oflarvae (mg) :t-"' ...,,., ::!' = C I Initial number of larvae ..., VI , \0 v ..sl 'I) \0 \t f\ ()' (f o' 0 o' o* C) o* o' o* ()' ()' Average Percent weight per reduction -3.() 7. - initial from control 0 *"' 'Z.\ number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: .J\__ Comments: ge 28 of98

  • (Ill Page 3 of6 ;)e :_n*-h*n-m"-'*'-Ten-Jng-Sal-"'lon-,,-,n<.

Species: Pimephales promelas Date: <fk IO-o\ Client: TVA I Sequoyah Nuclear Plant-Non-treated Survival and Growth Data Day 45.2% 72.6% 100% M N 0 p Q R s T u v w X 0 /{) /() ID /{J /Q 10 /Q /D fO 10 'D 10 1 /D /{) 10 /0 10 10 10 tO 10 10 1/J 10 2 rD 10 JD It> 10 tO 10 tO lt> lO I () ID 3 10 /D JQ /Q 10 10 /0 JO lD ID /0 10 4 J() IO fb IO tO I() fD tO ID 10 /() 10 5 LO ID 10 10 IO IO IO 10 10 10 10 10 6 /0 /() /0 10 ID 10 /Q /D /D /D /0 10 7 4'c( IO /o lSI'\ IO 10 10 /0 /Q 10 10 ID tQ A-Pan weight (mg) Tray color 15.S9 I"{.S1 *o.'U. 1\.f.lB 1'{.35 1'1.9.1 13.S(p 13 13.SS 13.83 ll.f.31o Analyst: Date: B-Pan +Larvae weight (mg) ,..,..Lr l,\. +1 'Jb.SS d.D.l>J IJ. l.D*I3 ).D. I) ,q,f>) dJ>.Oij )o.il+ Itt. tb Analyst: Date: DJ..* 13

  • C -Larvae weight (mg) =A-B S.!.'-t "'*"' c.*'" '-.I\?, ;.tS s.l't -s ,,'\ lcJ.OS ,.'-\1.

6,,, Weight per initial number bot, ;,._'V of larvae (mg) '"' ""'" "'"" c,"' c..,.,,., 4 ""' = C I Initial number of larvae \JI "' "' c; t; ... \1 ()" ()' o* ()' o* ()' o* o* C>. o* Average Percent weight per reduction \.7.1. o.-s&'-1 S.'\7. o.\.,1'\ o.o7. initial from control number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. . Calculations and data reviewed: j,__ Comments:

  • *
  • tl 'iii Page 4 of6 -,.,-,.,-m,-, .. -,r,-,..-.... -, .. -.,..-.,.-,ln<.

Species: Pimephales promelas Date: Client: TVA I Sequoyah Nuclear Plant-Non-treated Survival and Growth Data Day 100% Intake y z AA BB 0 ID I() ID IO 1 10 10 10 10 2 /D 10 10 LO 3 10 I() (0 )0 4 10 ID 10 10 5 10 10 lO to 6 /D 10 /0 ro 7 to IC 10 '1'.{ A= P11n weight Tray color code:: ':f-QES N.OB 13.'\0 ILlY Analyst: Date: 07.-\ Q-()CI B =Pan+ Larvae weight (mg) (v\Lt" lo.30 ;}.b. OJ. Jtt,ql Analyst: Date: -oq C =Larvae weight (mg) =A-B e,.."t't "'. \ z. Weight per Initial number ,'V ""'.., of larvae (mg) ...,tv "' = C I Initial number of larvae \t " ()' ()' o* o* Average Percent weight per reduction 0.sq1 3 .'\i. initial from control number of (%) larvae (mg) Comment codes: c =clear, d = dead, fg = fungus, k = killed, m =missing, sk = sick, sm =unusually small, lg = unusually large, d&r =decanted and returned, w = wounded. Calculations and data reviewed: .dJ._ Comments: '"C (.;.) .... Q ...., \C QC) * * * * "& Environmental Testing Solutions, Inc Coaceatralioa (%) Replicate A Control B c D E F G H I 22.6% J K L M 45,2o/a N 0 p (J R s T u 100°/e v w X y J oocv. lotake z AA BB OotraliJOl: Dunnett's MSD -valae: PMSD: !!!.!W;. Dunnett's MSD value: PMSD: lailialaamber of ..,. .. 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 0.1030 16.6 0.0575 9.3 Fiaal **mber of larvae 10 10 10 10 lO JO 10 10 10 10 10 10 10 10 10 9 10 10 10 10 10 10 10 10 10 10 10 9 A*Pu,..;pt (me) 14.32 14.28 13.41 14.05 13.20 13.91 14.17 15.90 14.51 14.20 14.49 13.75 15.59 14.57 13.92 14.78 14.35 14.91 13.57 13.86 13.99 13.55 13.83 14.36 14.08 13.90 12.74 14.39 PMSD* TVA I Sequoyah Nuclear Plant, Outfall lOt -Non-treated February 10-17,2009 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Not for Compliance Assessment, Internal Laboratory QC B*Paa-tLanK Larvu wac** (me) Wc:ipt I S*niriac M.eaa weie:*t I Codkieat ol Weigllt/ Jailialaumber welpt(mg)

  • A-B ** ...,.,. of ...... (mg) Sarvhiaaa*mber of <M-o* **P* ,... *"""'c &flanae (mg) latvve(mll)
      • WoflarTul('lo) 20.76 6.44 0.644 0.644 21.06 6.78 0.678 0.621 8.2 0.678 19.38 5.91 0.597 0.597 19.68 5.63 0.563 0.563 19.50 6.30 0.630 0.630 20.19 6.28 0 628 0.639 4.7 0.628 20.33 6.16 0.616 0.616 22.73 6.83 0.683 0.683 21.21 6.70 0.670 0.670 20.99 6.79 0.679 0.676 7.9 0.679 20.61 6.12 0.612 0612 2l.l8 7.43 0.743 0.743 21.73 6.14 0.614 0.614 20.76 6.19 0.619 0.627 7.4 0.619 20.85 6.93 0.693 0.693 20.03 5.25 0.583 0.525 20.12 5.77 0.577 0.577 20.13 5.22 0.522 0.584 9.6 0 522 20.15 6.58 0.6.58 0.658 19.65 5.79 0.579 0.519 20.04 6.05 0.605 0.605 20.97 7.42 0.742 0.6!9 14.2 0.742 19.76 5.93 0.593 0.593 19.70 5.34 0.534 0.534 20.30 6.22 0.622 0.622 20.02 6.12 0.612 0612 1.6 0.612 18.73 5.99 0.599 0.599 19.92 5.53 0.614 0.553 Minimum Significant Difference Percent Minimum Significant Difference Project *umber* 5246 ---*-***---

.. r \ Mae sarvival Mcaal\'Cight/ Coel cicot of PtrU:D1 rtdudiotl fr0111 ("'o) lailial .. mber or varialioa 1M-...ap. cntruJ("l'*) la,....(mg) pviAiliU,...*t>oorofla,...u) l%1 100.0 0.621 8.2 Not applicable 100.0 0.639 4.7 .J.O 100.0 0.676 7.9 -8.9 97.5 0.613 11.2 1.2 100.0 0.584 9.6 5.9 100.0 0.619 14.2 0.3 97.5 0.597 5.1 3.9 PMSD is a measure of test precision. The PMSD is the m1mmum percent difference between the control and treatment that can be declared statistically significant m a whole effluent toXICity test Lower PMSD bound detennined by USEPA (lOth percentile) 12%. UJlper PMSD bound detennined by USEPA (90th percentile)= 30%. Lower and upper PMSD bounds were detennined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET VariabilitY,Study (USEP A, 200 Ia; USEP A, 200 I b) USEPA. 2001 a, 200Jb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. ""C N Q """l \0 QCI TV A I Sequoyah Nuclear Plant, OutfalllOl -Non-treated .ETS * . . .. * * " ""-#, ---4,. *.** . "#/}-February 10-17, 2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 2/10/2009 Test ID: PpFRCR Sample ill: TV A I Sequoyah Nuclear Plant, Outfall 101 End Date: 2/17/2009 LabiD: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% 1 2 3 4 D-Control 0.6440 0.6780 0.5970 0.5630 11.3 0.6300 0.6280 0.6160 0.6830 22.6 0.6700 0.6790 0.6120 0.7430 45.2 0.6140 0.6190 0.6930 0.5250 72.6 0.5770 0.5220 0.6580 0.5790 100 0.6050 0.7420 0.5930 0.5340 Transform: Untransforlned !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6205 1.0000 0.6205 0.5630 0.6780 8.174 4 0.6453 1.0000 11.3 0.6393 1.0302 0.6393 0.6160 0.6830 4.664 4 -0.439 ' 2.410 0.1030 0.6453 1.0000 22.6 0.6760 1.0894 0.6760 0.6120 0.7430 7.934 4 -1.298 45.2 0.6128 0.9875 0.6128 0.5250 0.6930 11.220 4 0.181 72.6 0.5840 0.9412 0.5840 0.5220 0.6580 9.582 4 0.854 100 0.6185 0.9968 0.6185 0.5340 0.7420 14.226 4 0.047 Auxiliary Tests Statistic Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01) 0.95722824 Bartlett's Test indicates equal variances (p = 0.69) 3.06386232 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV 1U MSDu MSDp Dunnett'sTest 100 >100 I 0.10304414 0.1660663 Treatments vs D-Control Point IC05 ICIO IC15 IC20 IC25 IC40 IC50 % 45.035 >100 >100 >100 >100 >100 >100 SD 95%CL(Exp) Linear Interpolation (200 Resamples) Skew 2.410 0.1030 0.6453 1.0000 2.410 0.1030 0.6128 0.9496 2.410 0.1030 0.6013 0.9318 2.410 0.1030 0.6013 0.9318 Critical Skew Kurt 0.884 0.42652272 -0.0353657 15.0862722 MSB MSE F-Prob df 0.00375797 0.00365631 0.43092009 5, 18 sqnl01_02-I0-09data '"C (1) YJ YJ Q ...., \C QC) TV A I Sequoyah Nuclear Plant, Intake-Non-treated iETS February 10-17, 2009 Statistical Analyses c) Environmental Testing Solutions. Inc. Larval Fish Growth and Survival Test-7 Da;t Growth Start Date: End Date: Sample Date: Comments: Cone-% D-Control 100 Cone-% D-Control 100 2/10/2009 211712009 1 0.6440 0.6220 Mean 0.6205 0.5965 Tests 2 0.6780 0.6120 N-Mean 1.0000 0.9613 TestiD: LabiD: Protocol: 3 0.5970 0.5990 Mean 0.6205 0.5965 PpFRCR ETS-Envir. Testing Sol. FWCHR.-EPA-821-R-02-013 4 0.5630 0.5530 Transform: Untransformed Min 0.5630 0.5530 Max CV% 0.6780 8.174 0.6220 5.112 Shapiro-Wilk's Test indicates normal distribution (p > 0.01) F-Test indicates egual variances U! = 0.43l Hypothesis Test ( 1-tail, 0.05) SampleiD: Sample Type: Test Species: N 4 4 Statistic 0.95853066 2.76694155 MSDu t-Stat 0.811 MSDE Homoscedastic t Test indicates no significant differences 0.05749647 0.09266151 Treatments vs D-Control Point IC05 IC10 ICI5 IC20 IC25 IC40 IC50 % >iOo >100 >100 >100 >100 >100 >100 SD 95%CL(Exp) Linear Interpolation (200 Resamp1es) Skew TV A I Sequoyah Nuclear Plant, Intake DMR-Discharge Monitoring Report PP-Pimephales promelas 1-Tailed Critical 1.943 Critical 0.749 47.4672279 MSB 0.001152 MSD 0.0575 MSE 0.001751 Isotonic Mean N-Mean 0.6205 1.0000 0.5965 0.9613 Skew Kurt -0.2030801 -0.8613815 F-Prob df 0.44827035 1, 6 sqnl01_02-10-09data ""= nl -E * * * < ** ',, Environmental Testing Solutions, Inc. Concentration Control 11.3% 22.6% 45.2% 72.6% 100% 100% Intake TVA I Sequoyah Nuclear Plant, OutfalllOl -Non-treated February 10-17,2009 Pimephales promelas Chronic Whole Effiuent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses 5246 I I I I I f I I I I _, ____ _ _) Envll'l)l'lmentiiTe-1tlng Solutions, Inc:. Species: Pimephales promelas Client: TVA I Sequ()yah Nuclear Plant-Non-treated 11.3% 22.6% 45.2% 72.6% 100% 100% Intake lage 35 of98 Page 5 of6 Date: C1*10 *o'\ .:. .. J., .. S*-<, .. . ' . ) ) .. =, ... ...... .. Page 6 of6 Species: Pimephales promelas Date: 01*lD-O'\ Client: TV A I Sequoyah Nuclear Plant-Non-treated Concen-Parameter tration 11.3% 22.6%

  • 45.2% I 72.6% !
  • i ' 100% -I -I 100% I Intake ; I lage 36 of98 tage 37 of98 .. ... 11 !Jen -,-,,.,.-""-""'-,T...,-.,.-

.. ,-*** -** -In<. Page 1 of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA Facility: Sequoyah Nuclear Plant NPDES #: TN0026450 Project#: Dilution preparation information: Dilution prep (%) 11.3 22.6 Effluent volwne (mL) 282.5 565 Diluent volume (mL) 2217.5 1935 Total volume (mi..) 2500 2500 Test orl(anism source information: 45.2 1130 1370 2500 Organism age: < 24-hours old Date and rimes organisms were born 0'1* 0'\*t>'\ between: Culture board: nt*CJ;..O'\ A 72.6 100 1815 2500 685 0 2500 2500 10$0 'TI.) l't!J$ Replicate number: I I 2 I 3 I 4 I 5 I 6 I 1 I 8 I 9 I 10 Culture board cup number: ' I "'1..111..1 '"!:a I-' I1'!JI'l.S l't-1 Transfer bowl information: pH= '1.1.:.1 SU Temperature= "t"\ ,, oc Daily renewal information: Day 0 2 3 4 5 6 7 Date Test initiation, renewal, or termination time Control water batch used County: Hamilton Treatment: Non-treated Outfall: 101 Comments: Test information: Randomizing template color: Incubator number and shelf location: YWTbatch: Selenastrum batch: Sample numbers used 'Z.B\ 0\ Analyst Control information: 1 2 Acceptance criteria Summary of test endpoints: %of Male Adults:

  • 7-day LCSO )tM7. % Adults having 3ra Broods: % Mortality: , *** Mean
  • .* :* %CV: (...17.

3.37. If ('( . t I *-* I ' I I I I I I I I I I I I I I I * * * $ .!l Species: Ceriodaphnia dubia Page 2 of7 Client: Seguoyah Nuclear Plant-Non-treated Date: 0 Z*IO-o'\ CONTROL-1 Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 (") C) 0 0 o* 0 0 0 Adult mortality \..... '-\..._ L L. L \,_ L L L_ 2 Young produced {") ('") 0 () 0 0 c 0 0 0 Adult mortality '--L-L L L L \,_ L L.. L.. 3 Young produced 0 0 0 0 0 0 0 0 0 () Adult mortality L "-L_ L '--'--L-L_ L-"-4 Young produced .., s s "-\ \o '-\ '-\ '-l '-{ s Adult mortality '-L. \.... \,_ '--\.... L L... \.._ l_ 5 Young produced l \ \?,_ 1\ \1... \ '-\'-\ \ \ \l. Adult mortality \.._. L. '--L L '-'-\..._ '-L... 6 Young produced 1\o \'-\ l"5 \I.\ \\.-\1 l'-\ l\... \S Adult mortality '-'L.. '-L L L '-\.._ '-7 Young produced Total young produced a'-\ 3\ .3t> o'-\ '2'\ 3\ 3o 3l. Final Adult Mortality '-'-'--'-'-'-'--'--"---'-X for 3'd Broods X.. 2S -,.c:.. Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: I Mean Offspring/Female: I CONC: 11.3% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced a D a 0 0 0 0 0 0 0 Adult mortality '-L L L L L L \,_ L L 2 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L L L L L.. L L L '-3 Young produced D D C) Cl (') 0 0 a 0 0 Adult mortality L l L... L L L L L \..._ '--4 Young produced \,Q '-\ 5 .'--\ '-\ '-\ 4 s Adult mortality L L-L-L. \,__ \._. \._ L-L '-5 Young produced \1. \1. *3 1\ 14 l"t. 1'1 .. \ \ I"L Adult mortality L \._ L.. '-'-'-\._ '--'-'---6 Young produced \'5:. ,, 1\ \"::, \""'\ '"" '"" t.S Adult mortality '--\.._ '--L L \._ L_ '--'-!** "-7 Young produced Total young produced 35 .!6 35 . a'-t 2)D 3"2.. Final Adult Mortality \..;. '-\._. .\..,.,. *\;..__* L .* .. *\..;..._ L.. = *= Note. Adult mortahty (L hve, D dead) / .. :. Concentration: % Mortality:

  • -07. Mean Offspring/Female:

..32. (, % Reduction from Control: -t,'\7. .l : N ...Sl

  • <It
  • 110 ,)

... -... -,.-uuo"' In<. Species: Ceriodaphnia duhia Page3of7 Client: Seguoyah Nuclear Plant-Non-treated Date: ____ _ CONC: 22.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced (') 0 a n (') {") a 0 0 a -"'-Adult mortality L L L.. L \....... '-\..._ L.. "-\........ 2 Young produced 0 0 0 D C\ D 6 0 C'\ 0 Adult mortality L. \._ L L L L L \..._ l_ L 3 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L '-L L '\.._. \.._ L '-'-4 Young produced I-{ '-\ 'S 'S "5 "4 \.a s Adult mortality '-L. L '--'-'-L L. L '--5 Young produced \\ ,,_ \1... lo \ "'L \1... l"-\ l 'L Adult mortality \.-\._ L '-L. \..._ L. \._ L '-6 Young produced I\.. \ '-\ I\ \'S II I 0 \S '-S ,, 1\ Adult mortality '-'-'-L_ \._ '-L '-'-L.. 7 Young produced Total young produced as .3\ 3.?,) 32. 35 3S .3\ 3S Final Adult Mortality \ \ L L L L.. '--'-L l Note: Adult mortality (L =live, D =dead) Concentration: % Mortality:

07. Mean Offspring/Female:

a-o.s % Reduction from Control: *"\.1?. CONC: 45.2% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced () 0 0 0 0 0 0 0 0 0 Adult mortality L L-\._ L L L L \..._ L L 2 Young produced 0 0 0 0 0 0 6 0 0 0 Adult mortality L L L L L L L L L '-3 Young produced 0 0 D 0 D 0 a 0 () (\_ Adult mortality L L-L. \..._ L L L L L \.... 4 Young produced ..., "5 "-\ l.\ \p "-\ '.t. s s Adult mortality L L L \.._ '-L \,_ L._ \,_ '--5 Young produced 11 .... I\ 14 1'2. rL \ 1.. l"t. 11 .. 1.3 Adult mortality L 'L '-L \.._ \...... L L. \...,... \_., 6 Young produced \\.:, It\ \$ \(a l I<< 1"'1 I\.:, lei IS Adult mortality L L L L L \._ L. L.. L. L. 7 Young produced Total young produced 33 .32. ol.. .35 35 Final Adult Mortality L '-\. L L L \...... \,_ '-'--Note: Adult mortality. (L = live, D = dead) Concentration: % Mortality: " Q1. Mean Offspring/Female: .J'!._2 %Reduction from Control: .u IJ ll u u I . age 40 of98 I Page 4 of7 Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant-Non-treated Date: OZ*fO*o'\ CONC: 72.6% Survival and Reproduction Data *. Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced a D 0 0 0 0 D 0 0 c Adult mortality L L L L L '---L L L \..._ 2 Young produced 0 _0 D 0 0 0 (') () 0 0 Adult mortality \.... \._ L \.._. L.. L L.. L L. L 3 Young produced n 0 0 0 {) 0 0 0 0 0 Adult mortality L '--'--L.. L_ L_ \..._ L \.._ L-4 Young produced '--\ I.. '5 s '4 ., '-\ \p \, s Adult mortality L L L \._ \.._ "--'-'-'-\,..__ 5 Young produced -11-\C:, \"'L \3_ Adult mortality "-'-L \_ L '-L L L L.. 6 Young produced \&. \S \1 \\...,. '" ,-, tL.. Adult mortality '--\.._ L '--'-\._. L L_ L \..... 7 Young produced '0 Total young produced e.s .!>r 3t.. 31 Final Adult Mortality \.._ '\.... \_ '--\.._ '-'--\. '--\.._ Note: Adult mortality (L =live, D =dead) Concentration: % Mortality: ()1. Mean Offspring/Female: 3-5. to %Reduction from Control: CONC* 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced () 0 0 0 0 0 0 0 0 0 Adult mortality L-L L L L L L L L \_ 2 Young produced () (\ () 0 ('j 0 0 0 0 0 Adult mortality \,_ L L L L L... L L L L 3 Young produced 0 0 0 0 0 0 a 0 (') 0 Adult mortality L L L L.. L L L L_ 'L. L. 4 Young produced 4 (,c '5 s , s s lo t.. Adult mortality L. '-L L L \..._ L '-\_ '--5 Young produced \L.. \U. \"\ \"L \1.. \'l ... \:?. II..\ Adult mortality L L \....... \._ L L L.. \._ L L 6 Young produced ,., ltl ,., \'\ '2.D 1\..o Adult mortality L L-'-L L L L L \..._. '-7 Young produced .... Total young produced .31 l'. :?Jl.. 3l.. a'\ 31.. .. FinaiAdu!t Mor(alitv L '--L. L L \_ L. \...-'--'-Note: Adult mortality (L =live, D =dead) Concentration: % Mortality:**

07. Mean Offspring/Female:

.31.. 't %Reduction from Control:

  • f I! I J I ! I' l' ( I: u 41 of 98 * "' <a *llo.------

,'1) Envlronm.ntiiTesUng Solul:loru:,lnt. Species: Ceriodaphnia dubia Page 5 of7 Client: Seguoyah Nuclear Plant-Non-treated Date: _ __ _ CONTROL-2 Survival and Reproduction Data Replicate number Day 1 2 3 '4 5 6 7 8 9 10 1 Young produced n 0 0 D D () 0 0 0 0 Adult mortality L L L \...._ .__ '--'--L_ L L 2 Young produced 0 0 0 0 a a a_ 0 a a Adult mortality L \....... L \.._ L L L. L L L 3 Young produced *a 0 D 0 0 a 0 0 a 0_ Adult mortality L L L L L-L L. '-L l.._ 4 Young produced s '-{ L{ '-\ '-\ .-:; s 3 Adult mortality L '-\_. '-'-L. '--'--L. L 5 Young produced I \ ,,_ l'-\ \L.. \""2... I\ -\ \ \ Adult mortality L. '-l.-'--'-L L \._ L L-6 Young produced \\. -' IY ,, 1'-\ \'5 II,. 1'-i IS Adult mortality '-L '--\.._ L L_ L \..._ L L 7 Young produced Total young produced o\ 33 ¢1. ,3\ Final Adult Mortality_ \._ L. L.. L L-'--'--L L l Note: Adult mortality (L =live, D =dead) Concentration: % Mortality: I 1'11. Mean Offspring/Female: I 31*1 coNe: 100% Intake Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced [') 0 a_ 0 a_ 0 0 a 0 a Adult mortality j._.. '--L L L-L L L. L L 2 Young produced LJ {) 0 0 () 0 0 0 0_ 0 Adult mortality \....... L L L L L L L L '--3 Young produced n (\ () 0 0 0 0 0 0 0 Adult mortality \_ L '--L-L.. '-l.._ \_ \.._ L 4 Young produced '-t s 1.:. 5 'S '-\ s L.\ Adult mortality l.... L L-l-L L. L L-L-'--5 Young produced \1. 10 l'-\ l\ \ "1-Adult mortality '-'--'--L L L.. \.._ L L l...... 6 Young produced \1 20 tR '" \8 2\ ,., '" l'i_ Adult mortality \_ '-L. \.... '-'--'--'-'--"--7 Young produced Total young produced 0, 3, 4t> .,e,$ 3'-\ 31 oS Final Adult Mortality _l '-\._ \._ \_ '-L L L L Note: Adult mortality. (L = live, D = dead) Concentration: %Mortality:

07. Mean Offspring/Female:

.3$.1 %Reduction from Control:

  • 1'2. .&.1 .. . _31.

"'C N c ...... 00 .ETS * * * @ ;',ffi'fifi;.l!: '****." *'*!.:::'

.-. ._) Envlronmenlal TesUng Solutions, Inc. Control-1 11.3% 22.6% 45.2% TVA I Sequoyah Nuclear Plant, OutfalllOl-Non-treated February 10-16,2009 Verification of Ceriodaphnia Reproduction Totals 72.6% 100% Control-2 100% Intake (1el ""' w 0 ..., \C * * * @ Environmental Testl,ng Inc. Concentration

(%) I 2 3 Control-1 34 31 31 11.3% 33 33 35 22.6% 35 31 33 45.2% 35 35 33 72.6% 35 34 37 100% 37 34 39 Control-2 32 31 31 100% Intake 34 37 35 Outfall101: Dunnett's MSD value: 1.703 PMSD: 5.3 Duonett's MSD value: 1.269 PMSD: 4.0 TVA I Sequoyah Nuclear Plant, OutfalllOl -Non-treated February 10-16,2009 Ceriodaphnia dubio Chronic Whole Effluent Toxicity Test EP A-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses 4 30 31 32 32 34 38 33 33 MSD= PMSD= Project number: 5246 UJ* r\1 .. -.,g_ -II Replicate number Survival Average reproduction Co'tl'ffcient of Percent reduction from (%) (offspring/female) variation (Vo) pooled controls (%) 5 6 7 8 9 10 34 35 29 31 33 32 100 32.0 6.1 Not applicable 35 34 30 32 31 32 100 32.6 5.3 -1.9 35 35 31 35 33 35 100 33.5* 5.1 4.7 36 35 35 33 35 33 100 34.2 3.8 -6.9 35 38 36 37 36 34 100 35.6 4.0 -11.3 36 39 36 35 39 36 100 36.9 4.9 -15.3 33 31 33 32 31 30 100 31.7 3.3 Not applicable 37 40 35 34 37 35 100 35.7 5.8 -12.6 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. LowerPMSD boWJd determined by USEPA (lOth percentile) = 13%. Upper PMSD bound determined by USEP A (90th percentile) = 4 7%. Lower and upper PMSD boWJds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 200lb). USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program .. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EP A-821-B-01-004 and EPA-821-B-0 1-005. US Environmental Protection Agency, Cincinnati, OH. (JQ t Q ...., 00 ., * '*"*' ... *. February 10-16,2009 . E TS TV A I Sequoyah Nuclear Plant, OutfalllOl -Non-treated i __ --'*-.* :, "** Statistical Analyses ;,) Envlronmentai.Testtng Solutions, Inc. Survival and R!:£roduction Test-Reproduction Start Date: Vl0/2009 TestiD: CdFRCR SampleiD: TV A I Sequoyah Nuclear Plant, OUtfall 1 01

  • End Date: 211712Q09 Lab ID: ETS*Envir.

Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaplmia dubia Comments: Cone-% 1 2 3 4 5 6 7 8 9 10 D-Control 34.000 31.000 31.000 30.000 34.000 35.000 29.000 31.000 33.000 32.000 tl.3 33.000 33.000 35.000 31.000 35.000 34.000 30.000 32.000 31.000 32.000 22.6 35.000 31.000 33.000 32.000 35.000 35.000 31.000 35.000 33.000 35.000 45.2 35.000 35.000 33.000 32.000 36.000 35.000 35.000 33.000 35.000 33.000 72.6 35.000 34.000 37.000 34.000 35.000 38.000 36.000 37.000 36.000 34.000 100 37.000 34.000 39.000 38.000 36.000 39.000 36.000 35.000 39.000 36.000 Transform: Untransfonned !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D*Control 32.000 1.0000 32.000 29.000 35.000 6.074 10 34.133 1.0000 11.3 32.600 -1.0188 32.600 30.000 35.000 5.254 10 -0.806 2.287 1.703 34.133 1.0000 22.6 33.500 1.0469 33.500 31.000 35.000 5.122 10 -2.014 2.287 1.703 34.133 1.0000 45.2 34.200 1.0688 34.200 32.000 36.000 3.850 10 -2.954 2.287 1.703 34.133 1.0000 72.6 35.600 1.1125 35.600 34.000 38.000 4.016 10 -4.833 2.287 1.703 34.133 1.0000 100 36.900 1.1531 36.900 34.000 39.000 4.856 10 -6.578 2.287 1.703 34.133 1.0000 Auxili!!;!! Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > O.Q1) 0.864555061 1.035 -0.055127545 -1.11981957 Bartlett's Test indicates !:}Ual variances (I!= 0.88! 1.7678653 15.08627224 Hypothesis Test (1-tail, 0.05! NOEC LOEC ChV TU MSDu MSD2 MSB MSE F-Prob df Dunnett's Test 100 >100 1 1.703244044 0.053226376 34.22666667 2.774074074 5.4E-08 5,54 Treatments vs D-Control Point % SD 95%CL Linear Interpolation (200 Resamples) Skew IC05 >100 IC10 >100 !CIS >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqnl01_02-10-09data

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.... 0 Environmental Tasting Solutions, Inc. TV A I Sequoyah Nuclear Plant, Intake -Non-treated February 10-16, 2009 Statistical Analyses Ceriod!!£1mia Survival and Start Date: 2/10/2009 Test ID: CdFRCR SampleiD: TV A I Sequoyah Nuclear Plant, Intake End Date: Sample Date: Comments: Cone-% D-Control 100 Cone-% D-Control 100 2/17/2009 I 2 32.000 31.000 34.000 37.000 Mean N-Mean 31.700 1.0000 35.700 1.1262 Lab ID: Protocol: 3 31.000 35.000 Mean 31.700 35.700 ETS-Envir. Testing Sol. FWCHR-EPA-821-R-02-013 4 5 6 33.000 33.000 31.000 33.000 37.000 40.000 Transform: Untransfonned Min 30.000 33.000 Max 33.000 40.000 CV% 3.342 5.763 Sample Type: Test Species: 7 33.000 35.000 N 10 10 DMR-Discharge Monitoring Report CD-Ceriodaplmia dubia 8 9 10 32.000 31.000 30.000 34.000 37.000 35.000 !-Tailed Isotonic t-Stat Critical MSD Mean N-Mean 33.700 1.0000 -5.466 1.734 1.269 33.700 1.0000

  • Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.901544869 0.868 0.809339798 1.387605747 F-Test indicates variances (p =: 0.06) 3.772277117 6.541089535 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.26901657 0.040032068 80 2.677777778 3.4£-05 I, 18 Treatrrtents vs D-Control Point % IC05 >100 IClO >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 ICSO >100 SD 95%CL Linear Interpolation (200 Resamples)

Skew sqn101_02-10-09data (JQ ""'

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  • Environmental Testing Solutions, Inc. Control 11.3% 22.6% 45.2% 72.6% 100% TV A I Sequoyah Nuclear Plant, Outfa11101 -Non-treated February 10-16, 2009 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Project number: 5246 ---t------

I Page 6 of7 Species: Ceriodaphnta duhia Client: Seguoyah Nuclea.r Plant-Non-treated 11.3% 22.6% 45.2% 72.6% '100% . 1-100% Intake age 47 of98 I I i I

  • i I ' I tration 11.3% 22.6% 45.2% 72.6% 100% 100% Intake 48 of98 Page 7 of7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant-Non-treated Date: Ci.-!o-d1
  • 0 1\lo " Page 1 of6 J Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: TVA Facility:

Seguoyah Nuclear Plant NPDES #:. TN0026450 Project#:

  • Dilution preparation information:

Dilution prep (%) l!J 22.6 Effluent volume (mL) 282.5 565 Diluent volume (mL) 2217.5 1935 Total volume (mL) 2500 2500 Test orflanism information: 45.2 1130 1370 2500 Organism age: LC\ H.o\Jitc; oLD Date and times organisms I bOO were born between: 72.6 100 1815 2500 685 0 2500 2500 Organism source: A 'it:))(. &A\<.\\ (J p cn.-<f\-0'\ Transfer bowl infonnation: pH= Temperature= oc l.(o 1.. 1."t*3 Average transfer volume: ...-Q. County: Hamilton Treatment: UV-treated Outfall: 101 Comments: ,, Each concentration was treated for 2 minutes with a UV sterilizer to remove _Qathf!genic interferences. Test information: Randomizin_g_ tero_ll_late: &t..\)e. Incubator number: 3& Artemia lot number: f6Jooc.fw Total drying time: ., ... 1\()\ltC> Date I Time in: ()t,. n*cf\ 1\\t) Date I Time out: Cl.*\A*Q'\ t\\0 Oven temperature: \oc't. Daily feeding and renewal information: Day Date 0 2 3 4 5 6 7 49 of 98 Morning feeding time Afternoon feeding time Test initiation, Control water batch used Sample numbers used Analyst Page2of6 Species: Pimephales promelas Date:

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  • 10 *o"\ Client: TVA /Sequoyah Nuclear Plant-UV-treated Survival and Growth Data Day CUNfROL 11.3% 22.6% A B c D E F G H I J K L 0 I'D IO 10 ID /Q /Q IO /Q to /Q ID IQ 1 /() 10 /0 /0 10 to IO /0 10 !D /{) 10 2 It> ID IO 10 10 ID 10 10 10 /0 I'D 10 3 lt:J IO 10 to /0 IO 10 IO 10 /6 tO 10 4 ID 10 /Q IO /Q 10 /Q 10 IO 10 10 10 5 IC tD 10 /0 ID ID /Q /Q ID /0 10 tO 6 /Q /0 /Q /() /0 /Q /Q /Q /(j IQ IO 10 7 10 10 10 /Q IO 10 10 10 ID 10 /0 10 A = Pan weight (mg) Tray color code:: OVo..N5-e Analyst: LA-e l"f.l(c t3 .sa It.! .II lt{.\{3 13.eq IS.lcJ l4.3'-t 14.01 l5. 1.2.

0'2.-10-Dq B =Pan + Larvae weight (mg) f'LI"' J,b. Jq, bs Analyst: IY.M ll-tJ. J.l, '3b .;lJ.'U ll-l4 ;)l>. lq.8f _l)_ate: C =Larvae weight (mg) ::A-B (,.\\ "'* 1-'2.0 1.1.1\ (,.1..4 ;."(o Weight per initial number e., :>.."-..).. :> of larvae (mg) w,o ,o " " = C I Initial number of larvae " 'II \1) ...0 " Jli, "' \t 'I> (;) o* o* o* o* o* o* o* o* o* t>' Average Percent weight per reduction o.lo '2 1 o.'1' C> -n .. t7 .. -, .... \ 7. initial from control number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k ==killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r == decanted and returned, w = wounded. Calculations and data L *.* *.* Comments: .. . * .. -SO of98

  • .. $

.. -.. Species: Pimephales prome/as Date: cn.*to-o'\ Client: TVA I Sequoyah Nuclear Plant-UV-treated Survival and Growth Data Day 45.2% 72.6% 100% M N 0 p 0 R s T u v w 0 -/0 IO /D fO /Q ID 10 IO to 10 10 1 /D /0 /0 10 10 1C /0 IO 10 10 10 2 10 {0 /0 /0 /(J IO 10 lD to ID I CJ 3 /D 10 /D /D ID tO (t:J IO 10 /0 10 4 10 10 10 10 10 IO 10 /6 '0 10 10 5 /D tD IO l(J to lD /Q tO lQ /(J 10 6 10 /D 10 IO ID /D ID ID lb ID )0 7 ID 1St'\ /0 I {) /D /t) /{) ID /() to 10 f(J A-Pan weight (mg) Tray color code,:: OV'a.N9C *Analyst: LM lS.IQ; II{.I{S 13.l£l8 13.01 11-t.'B l\{.11 i Date: DZ-IC\-f\C\ B = Pan + Larvae weight (mg) ";).l*iJ d0-Y8 )b. ;).d. ;)b. to ]8*1-l ;J.I.3J

lb. J,D.4clf d-().18 Analyst
Date
o?.* 1 C-Larvae weight (mg) =A-B , . I lei S.11 5*1\

c,.11D (, .1.1... 1,."\1 c,:z.--z. S.llo Weight per initial number A"-;:,."' "\Q of larvae (mg) ;:o "' "' t1o.. f\1\11 = C I Initial number oflarvae "" ... 14 t.; <, ... \D \1 o* o* o* o* C) o* ()' o* (j Average Percent Page 3 of6 X 10 to 10 10 10 10 10 10 l'-\-'l.lq

l.l-1'1 tt.i& o* weight per reduction

-o.s7. o .t.s'-{ ,'\ 7. initial from control o. (,04 0 ..... number of (%) larvae (mel Conunent codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg =unusually large, d&r = decanted and returned, w =wounded. Calculations and data reviewed: £ Comments: .. 51 of98

  • Ill ., 'iil *J> Page 4 of6 ::......,..-.-.,..-,,.-

... -,r .. 52 of98 Species: Pimephales promelas Date: 0""2. o't Client: TVA I Sequoyah Nuclear Plant-UV-treated Survival and Growth Data Day 100% Intake y z AA BB 0 10 10 10 10 1 /Q IO 10 10 2 tO I() ID 10 3 (O /() I D ID 4 10 10 10 10 5 /D tD 10 lO 6 JD 10 10 10 7 l$f"t ID 10 ID 'D A= Pan weight t Tray color code:: ""'""l(, . r Analyst: L&S l'Z..c:q Date: Ot-ln-nct B =Pan + Larvae weight (mg) IS',q, J1-53 ;)b.bo Analyst: Date: ol--1'& C = Larvae weight (mg) =A-B S,\., 1.,.() \ s.al.. 1.\1 Weight per initial number " ' " oflarvae (mg) t,'-\It> c.; = C I Initial number oflarvae o* '\)' I;)' Average Percent weight per reduction \. 8. 1. initial from control number of (%) larvae (me:) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk.= sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: J_ Comments: "'d J:Oi !10 (t> Ul w 0 ...., \0 00 * * *

  • lftl I!:(!;J1 0 Environmental Testing Solutions, Inc Co.ea&ntio*

(0/*) JlepiiW. Iaitialoumbuof Fiaala*llber rl A=Puwdpt lanK ,.., .. (mg) A 10 10 14.76 Control B 10 10 14.34 c 10 10 13.58 D 10 10 14.14 E 10 10 14.11 11.3% F 10 10 11.69 G 10 10 14.43 H 10 10 13.89 I lO tO 15.61 11.6% J 10 10 14.34 K 10 10 1407 L 10 10 13.22 M 10 10 14.67 N 10 10 15.85 45.2% 0 10 10 15.32 p 10 10 14.45 Q 10 10 13.68 R 10 10 13.01 72.6o/. s 10 10 14.93 T 10 10 14.71 u 10 10 14.64 v 10 10 13.95 100% w 10 10 14.41 X 10 10 14.26 y LO 10 14.62 100% latake z 10 10 12.97 AA 10 10 13.67 -----BB _10 -------JQ__ 13.43 --Oucraii!Ol: MSD= Dunnett's MSD value: 0.0838 PMSD= PMSD: 1!,3 IntiS;. Dunnett's MSD value: 0.0703 PMSD: ll.l TVA /Sequoyah Nuclear Plant, OutfalllOl-UV-treated February 10-17,2009 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Not for Compliance Assessment. Internal Laboratory QC B "" Po + Larvae Larvae waa*t (1111) Wdght/SIIn'ivilog Mcuweig.tf Codlicieac ofvariatioa WtiPt I laitial **mbru

  • A-8 ou-..orbrvae(mg)

Surviviae

      • ber of (Wr*ll...-tpertar¥1ri*J ofbrv .. (mg) brvoe(mg) -Deroel*"w)(Y*)

20.87 6.11 0.611 0.611 20.64 6.30 0.630 0.629 4.1 0.630 19.68 6.10 0.610 0.610 20.79 6.65 0.665 0.665 20.53 6.42 0.642 0.642 18.89 7.20 0.720 0.710 6.5 0720 21.72 7.29 0.729 0.729 21.36 7.47 0.747 0.747 22.25 6.64 0.664 0.664 21.74 7.40 0.740 0.676 6.6 0.740 20.43 6.36 0.636 0.636 19.85 6.63 0.663 0.663 21.83 7.16 0.716 0.716 22.07 6.22 0.622 0.608 13 9 0.622 20.48 5.16 0.516 0.516 20.22 5.77 0.577 0.577 20.10 6.42 0.642 0.642 18.72 5.71 0.571 0.632 7.0 0.571 21.32 6.39 0.639 0.639 21.47 6.76 0.676 0.676 20.90 6.26 0.626 0.626 20.49 6.54 0.654 0.654 3.9 0.654 20.88 6.47 0.647 0.647 2l.l4 6.88 0.688 0.688 20.29 5.67 0.567 0.567 18.98 6.01 0.601 0.618 10.9 0.601 19.53 5.86 0.586 0.586 __ 7.17 0.717 0.717 Minimum Significant Difference Percent Minimum Significant Difference Project number* 5246 A _II_ A. Mcusurvivll Meaaweigllt/ Coellld<Dtll( J'en:ellc rHuCCio* rrom (%) laitial .. mbcr ol variatioa (Mn11 ""Jill coatrol (0 1.) larvae (IDI) p.r u.itiai .......... u.,-.-J "{,J 100.0 0.629 4.1 Not applicable 100.0 0.710 6.5 -12.8 100.0 0.676 6.6 -7.4 100.0 0.608 13.9 3.4 100.0 0.632 7.0 -0.5 100.0 0.654 3.9 -3.9 100.0 0.618 10.9 1.8 ------PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (lOth percentile)

12%. Upper PMSD bound detennined by USEPA (90th percentile)

30%. Lower and upper PMSD bounds were determined from the I Oth and 90th percentile, respectively. of PMSD data from EPA's WET Interlaboratory Variability Study (USEP A. 200 Ia, USEP A. 2001 b). USEPA. 200 I a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-o l-G04 and EPA-821-B-01-GOS. US Environmental Protection Agency. Cincinnati, OH. !J'Q til ... 0 ...... \C QO TVA I Sequoyah Nuclear Plant, OutfalllOl-UV-treated

  • Ers * * . *. . . . **. * 'r..:Zf: +,?;0 1;.{G' February 10-17, 2009 Statistical Analyses Testing Solutions, Inc. Larval Fish Growth and Survival Test-7 Day Growth Start Dare: 2/10/2009 TestiD: PpFRCR Sample ID: End Date: 2/17/2009 Lab ID: ETS-Envir.

Testing Sol. Sample Type: Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: Comments: Cone-% 1 2 3 4 D-Control 0.6110 0.6300 0.6100 0.6650 11.3 0.6420 0.7200 0.7290 0.7470 22.6 0.6640 0.7400 0.6360 0.6630 45.2 0.7160 0.6220 0.5160 0.5770 72.6 0.6420 0.5710 0.6390 0.6760 100 0.6260 0.6540 0.6470 0.6880 Transfmm: Untransformed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat D-Control 0.6290 1.0000 0.6290 0.6100 0.6650 4.086 4 11.3 0.7095 1.1280 0.7095 0.6420 0.7470 6.537 4 -2.315 22.6 0.6758 1.0743 0.6758 0.6360 0.7400 6.623 4 -1.345 45.2 0.6078 0.9662 0.6078 0.5160 0.7160 13.860 4 0.611 72.6 0.6320 1.0048 0.6320 0.5710 0.6760 6.961 4 -0.086 100 0.6538 1.0393 0.6538 0.6260 0.6880 3.938 4 -0.712 Auxiliary Tests Statistic Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98361433 Bartlett's Test indicates equal variances (p = 0.37) 5.42555952 Hypothesis Test (l-tai1, 0.05) NOEC LOEC CIJ_V TIJ MSDu MSDp Dunnett's Test 100 >100 1 0.08379661 0.13322196 Treatments vs D-Contro1 Point IC05 ICIO IC15 IC20 IC25 IC40 IC50 % 41.450 >100 >100 >100 >100 >100 >100 so 95%CL(Exp) Linear Interpolation (200 Resamples) Skew TV A I Sequoyah Nuclear Plant, Outfall 1 0 I DMR-Discharge Monitoring Report PP-Pimephales prome1as !-Tailed Isotonic Critical MSD Mean N-Mean 0.6714 1.0000 2.410 0.0838 0.6714 1.0000 2.410 0.0838 0.6714 1.0000 2.410 0.0838 0.6312 0.9401 2.410 0.0838 0.6312 0.9401 2.410 0.0838 0.6312 0.9401 Critical Skew Kurt 0.884 0.19270246 0.87105609 15.0862722 MSB MSE F-Prob df 0.00540594 0.002417958 0.09521794 5, 18 sqnl01_02-J0-09data-uv (fQ (t> VI VI 0 ...... 1,0 QC TV A I Sequoyah Nuclear Plant, Intake -UV -treated : E*T* -a February 10-17,2009

,,, .c, '*. ,,, . ...:::lit Statistical Analyses 0 Start Date: 2/10/2009 End Date: 2117/2009 Sample Date: Comments:

Cone-% D-Control 100 0.6110 0.5670 2 0.6300 0.6010 Test ID: LabiD: Protocol: 3 0.6100 0.5860 Larval Fish Growth and Survival Test* 7 Day Growth PpFRCR ETS-Envir. Testing Sol. FWCHR-EPA-821-R-02-013 4 0.6650 0.7170 Transform: Untransformed Sample ill: Sample Type: Test Species: TV A I Sequoyah Nuclear Plant, Intake DMR-Discharge Monitoring Report PP-Pimephales promelas 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean 0.6290 1.0000 0.6100 0.6650 4.086 0.6290 1.0000 D-Control too 0.6178 0.9821 0.6290 0.6178 0.5670 0.7170 10.945 4 4 0.311 1.943 0.0703 0.6178 0.9821 Auxiliary Tests Statistic Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.85388428 F-Test indicates equal variances (p = 0.15) 6.91965199 Hypothesis Test (1-tail, 0.05) MSDu MSDp Homoscedastic t Test indicates no significant differences 0.07027929 0.11173178 Treatments vs D-Control Point IC05 ICIO IC15 IC20 IC25 IC40 IC50 % >100 >100 >100 >100 >100 >100 >100 SD 95%CL(Exp) Linear Interpolation (200 Resamples) Skew Critical Skew Kurt 0.749 1.514357215 2.37378198 47.46722794 MSB MSE F-Prob df 0.000253125 0.002616125 0.766281605 I, 6 sqnl 0 1_ 02-1 0-09data-uv '"0 !:" (JQ til 0'\ 0 ..... I *

  • Environmental Testing Solutions, Inc. Concentration Control 11.3% 22.6% 45.2% 72.6% 100% 100% Intake TVA I Sequoyah Nuclear Plant, Outfall101-UV-treated February 10-17,2009 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses '\246 Project number:---,,.....----..:..::-

Page 5 of 6 Species: Pimephales promelas Date: O"Z.*tO*ot\ Client: TVA I Sequoyab Nuclear Plant-UV -treated 100% Intake iETS Page 6 of6 Envlrcnnn!nht Testing Solutions. Inc. Species: Pimephales promelas Date: _ __:O::...:'L:::...*

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Client: TV A I Sequoyah Nuclear Plant-UV-treated Concen-Parameter tration I I 11.3% I 22.6% .I I: 45.2% I 72.6% I I 100% I I 100% I Intake I 'ge 58 of98

  • * " E-n-vlr-on-m-en-tai-Te-st-ing_Sol_u-tlo-n-s, I-nc. Ana!ym I Date analyzed 0 ?j 0 j q L b l a oratory contra standard:

Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pH/Ion Meter PageJL_ Page _I_ of ___J_ Iodide reagent:

1 Acid reagent: 'J:.f\i 3ob to bracket the chlorine levels of the samples. Reference standard True value (TV) Measured value (MV) % RS =MV /TVx 100 number (mg!L) (mg!L) (acceptable range= 90 to 110%) 56=

I 0.50 (), 41-3 Sample ID Sample characteristics Note: All samples were analyzed in excess of EPA holding time (15 minutes) unless otherwise noted. L b I a oratory contra standard: .< Reference standard True value (TV) Measured value (MV) %'ItS'.= j\1V I TV x 100 number (mg!L) (mg!L) (accep.tabk.rangc"" 9Q to 110%) I -l:> T'1Jf -o:so, Or$D'!> }00. 6 'c) I"' 'JI:-y\"..:::. age 59 of98 Reviewed by Date reviewed tJki 0

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  • f) Environmental Testing Solutions, Inc. Analyst I ML""-Date analyzed ol /11-/o9 7 I Calibration:

Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.!0 mg!L Meter: Accumet Model AR25 pH/Ion Meter Pagei_L Page_/_ of__)__ Iodide reagent: Tl\ I Acid reagent: "3'6{, Note: For samples with a residual chlorine of > 1.0 mg!L, the calibration range must be adjusted to bracket the chlorine" levels of the samples. L b I d d a ora ory contra stan ar : Reference standard True value (TV) number (mg!L) :trs "( .< 0.50 Sample ID Measured value (MV) (mg/L) Q, 1./64 s !.. 0 D L(J ,oo JC{q Sample characteristics '* . % RS = MV I TV x 100 (acceptable range= 90 to 110%) Note: All samples were analyzed in excess ofEP A recommended holding time ( 15 minutes) unless otherwisenoted. L b I d a oratory contro stan ard: Reference standard True value (TV) Measured value (MV) % RS = MV /TVx 100 number (mg/L) (mg/L) (acceptable range= 90 to UO%) _It\.<' *c .* ,., 0.50 )OJ. 4 T 3 lage 60 of98 Reviewed by Date reviewed L--...::0_'2..:::::..-- -'-'\'2...._-_,d\,_,___,

  • * 'I& -n-vlro_n_m-en-ta-1 -So-lut-Io-ns-.

In<-. Analyst I l Jg12? Date analyzed 01..-\ y-09 Calibration: Laborato control standard: Reference standard Total Residual Chlorine Page l 0 Page _j_____ of_'l._ (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pH/Ion Meter True value (TV) (mg/L) 0.50 Sample characteristics Measured value (MV) (mg/L) Iodide reagent: I JNCZ..3 z. 1 Acid reagent Il\ll2. WID % RS =MY /TV X 100 (acceptable range= 90 to 110%) Sample ID Sample characteristics Laborato control standard: Reference standard number Measured value (MY) (mg/L) c.. o .ococ '!I'Z.B '-O.oot"t'Z. % RS=MV /TVx 100 (acceptable range= 90 to 110%) Reviewed by Date reviewed {> j } Environmental Testing Solutions, Inc. Analyst I Date analyzed Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pHil on Meter Page_l..!.._L_I _ Page __1:_ of ...:L__ Iodide reagent: Acid reagent: Note: For samples with a residual chlorine of > 1.0 mg!L, the calibration range must be adjusted to bracket the chlorine levels of the samples. L b l a oratory contro stan d d ar : Reference standard True value (TV) Measured value (MV) % RS = MV /TVx 100 number (mg/L) (mg!L) (acceptable range= 90 to llOo/o) :Ct\JSSS1'1 0.50 0 qq,ln'/* Sample characteristics SampleiD Sample characteristics Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. L b l a oratory contro stan d d ar : Reference standard True value (TV) Measured value (MV) % RS = MV /TVx 100 number (mg!L) (mg!L) (acceptable range= 90 to 110%) 0.50 o.S0-:6 IDO.lo'{, age 62 of98 Reviewed by I Date reviewed I I I I I I I I I lage 63 of Analyst Date analyzed . o'8 . oq Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCOiL Titrate samples to pH= 4.50 S.U. Page _q_,_____ Page _ _:___of Time initiated 113 3D J Time completed Titrant normality an d 1 r d mu tip, ter eterm natwn: pH of Normality Normality (N) of H2S04 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na 2 C0 3 x 0.05)/E = (N x 50000)/100 ml sample water reference standard ml ml ml = 0.25/E =Nx500 =4.5 s.u. number number (E) (acceptable range= 0.0180-0.0220) :r f!. ,:n., :i) ... lSSI.R53 () .:, 19,.0 lo .Y. 1"3\\l... D.u-7 0. '2>:: 0-3n\ \ L b I d d a oratory contra stan ar : Reference standard True value Sample Alkalinity (MY) % RS=MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCQ,/L) (acceptable range (mgCaCOYL) (ml) ml ml ml = 90 to 110%) :r NSS ll2 'D :J. 100 100 10.0 lc .4-I e t:Fte B$1.. I o+crt.c Alkalinity %RPD= Total Multiplier (mg CaC03/L) {(S-D) /[(S+D)/2)} x 100 ml (acceptable range=+/- 10%) lo:O lo.'-t s (cO\ _L D lt .I lR3 M . ik atrzx sp e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mgCaCOYL) (mg CaCOYL) (ml) ml ml ml 50 ltlD tll3 11.0 IO !+ \10 Sample alkalinity (B) Measured spike value (MY) % R=MV /SVx 100 (mg CaC0 3/L) MV=A-B (acceptable range (mg CaC03/L) =75 to 125%) l.p?;, 4-1-C\40lc s amp* e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multi(llier CaC03/L) DZ-OS*CAP.., 1-hO too 39.?, lo. \ lD.Y. to3 02-n-:J.oq -l .... RY (.,.I lR .I lP3 1\1-DY .ua... i-!M:> 14.l# .3. i 3;l D\*25-a:::\ M\4"\ 1-\.,o 14.to {p,O loJ-o l*l-:t-0"1 A : Ol,'D.\0 . Uz_.t> {g;l. Ol.l1-oct8

j{p .\p (p.o {£.). 02-!i>-_QqQ 3<g .\p l.R .o tua.

H4.{c _k.O {o2. OZ.-03-o'1A -L--o.o 'lS <1.r:l. .... 1---IOO Reviewed by: Date reviewed: I Ol-a:l ..tf? 1 :1 l I j l ! ! ! Page 64 Analyst j'-----g Date amlyzed Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC0 3/L Titrate samples to pH= 4.50 S.U. Page \0 Page Ql Time initiated R Time completed Til171nr normallly .. . *.** I: nata*-ination: pH of Normality N. mality (N) of H 2 S04 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na 2 CU 3 ""' (N x ml sample water reference standard ml ml ml -0.25/E -==m 500 = 4.5S.U. number number (E) (acceptable range= 0.0180-0.0220) L b d d a ora ory contro stan ar : Reference standard True value Sample Alkalinity (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaC03/L) (ml) ml ml ml =90 to 110%) .:r lR o-:r-100 100 9.'1-ICJ.tp q,q lD .'4-lo?> rrzo Sample Alkalinity %RPD= Sample Sample ID volume End Total Multiplier (mg CaCOJIL) {(S-D) /[(S+D)/2)} x 100 number (acceptable range,;,+/- 10%) 01. -b3--C9\ P.::> .:Sa..£T'Su.J teA: s J Duplicate (B) J._ D u . 'k atriX spz e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCDJ/L) (m!! CaCO)!L) (ml) ml ml ml 50 100 B'l.l 1-B.O lc !t -Sample alkalinity (B) Measured spike value (MV) % R=MV /SVx 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) -\OD n . Ill ....U f l.lY1 117 \f

  • IJ s amp, e measurements:

Sample volume Begin End Total Alkalinity Sample number Samole ID (ml) ml ml ml Multiolier (mg CaC03/L) Oz ..-o ":l-OCi sa-VSw }OD I43.0'l -:sol) i)j) C\.1-tt>.tt' lt>O OCJam-1.12-I (Xzoh &st. Y.. I ts) () Q 1.1. -H '"'*2. 1'1-.1-mo_rshiL I.S 0'\ bL. tl'i A-e,"V) -.:21.'b \-1-l<t, tlJA-Gt.Blrlh z.o ldf\.IJ 1.&) [!">) '13 o'-\ 'IJA fbt"d"i/\D .BoO 015-0 o(CJ.C 4.0 foMwd .Jq.o St.'} 5.+ sq fY1oloS.ol \ 1.-,0,4.1--1"44.1 C\CO \)l)Q2o1-DZ.. 3 _:...-%.1 Hcl.2-5.\ _l--53 ** /U Reviewed by: Date reviewed: I 02.-0 'f-0'1 ] Analyst I {%1.. Date analyzed Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCOiL Titrate samples to pH= 4.50 S.U. Page _1_1 __ Page ___ of 3 Time initiated ... Time completed 154S"' Titrant normality and mu tiplier determination: pH of Normality Normality (N) of H2S0 4 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na 2 C03 x 0.05)/E = (Nx 50000)/ 100 ml5ample water reference standard ml ml ml =0.25/E =Nx 500 =4.5 s.u. number number (E) (acceptable range= 0.0180-0.0220) J:"t\155loS3 0.1 I.X.o 11.'1 ().OaiO ID-5 .. &Ill C..rre.t..-t'ioo*. l:).o-7 0.1 I Laboratory contro standard: Reference standard True value Sample Alkalinity (MV) % RS=MV /TV X 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaC()JiL) (ml) ml ml ml =90 to 110%) 100 100 C\74-loS qq qqq. Sample Alkalinity %RPD= Sample ID volume End Total Multiplier (mg CaC03/L) {(S. D) /[(S+D)/2)} x 100 (acceptable range=+/- 10%) 1¥-..\-hO 10.5 s + Duplicate (B) .L D -33.S' lu.O J,... M, 'k atrtx spr e recovery: ,Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaC03/L) (ml) ml ml ml IJ\1S.S.I.psY So \DD lb-'5 to.S l\b Sample alkalinity (B) Measured spike value (MV) % R=MV /SVx 100 (mg CaCO]!L) MV=A-B (acceptable range (mg CaCO]!L) = 75 to 125%) 11-1-s, l amp, e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Mult!J>iier (me CaCO]!L) D2-lb-Cf1 rn \1s.t-ho toO 5_9_ lOS Dt-6sw t-\..0 _L-0*0 6.1 ,.,""...ti 3., \ '"ho 3.1 CJ.'Z. 4>.( I Ln.J I o

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Testing Solutions. Inc:. Analyst Date analyzed a;1. *1'5 *CCf Alkalinity (SM 2320B) Matrix: Water, RL = 1.0 mg CaCOiL Titrate samples to pH= 4.50 S.U. Page 11> Page ?? of 3 -=---Time initiated I '-......... ......___ .. *I Time completed ,_ ____ t r d etermmatwn: itrant normality and multtp1Zer pH of Normality Normality (N) of H2S04 pH Factor or Multiplier Deionized Titrant check Begin End Total -(5 ml Na 1<..v3 ".n"\fli' -' (N x 5000q)/IOO ml sample water reference standard ml ml ml -0.25/E =4.5 s.u. number number on (acceptable range= 0.0180. 0.0220) L b 1 t d d a oratory contro s an ar : Reference standard True value Sample Alkalinity (MV) % RS =MV /TV X 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJiL) (ml) ml ml ml =90 to 110%) INS-!> tp61 100 100 3q.l 9.2 I(}.S l11-"ic"l-o Alkalinity %RPD= Begin End Total Multiplier (mg CaCOJIL) {(S-D) /[(S+D)/2)} x 100 ml ml ml (acceptable range= :1: 10%) D.O 4..1' (.., . -:r-1o.s s ':}o .J. Dup lie ate (B) 135"" U*g ..L D '11 Mit. 'k a rtx spz e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) _(JIIg_ CaCOJIL) (ml) ml ml ml 1 N 5o too lQ.i-lf02-ll.S 1o.S 120 Sample alkalinity (B) Measured spike value (MV) % R = MV I SV :x: 100 (mg CaCOJIL) MV=A-B (acceptable range (_m_g_ CaCOJ/L) =75 to 125%) .,, >-tq s 1 amp1 e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier ( mg CaCOJIL) oqb 2-t11-0J I lot> 'q ,?..- t:J ,0 IO.S Ia{ .1 3 ld:23_ 1.1 I {) b2 flCIJ I '6'\,_:,i '1-.o 1-4 l 15 J '2. ?ft.3 1-fJL.'L li-'1 D1b2J?:>. Olo ..3 0.0 \1/.Cj D1b2Jn.D ) .rf A 0\J i t.g,q q.) {)tjo 2 IL t lJ l 2 f:lt-) Oqoz-13.1/5 J 3 l.J:J lu-1-'1-u 0007. (fl. 6'k t __,.__ '1.0 -!....-*-:tz+ Reviewed by: Date reviewed: I 0'2 -IS I I I I I I I I I I I I I I I I tage 67 o Analyst Date analyzed . 16. D4 Alkalinity (SM 2320 B) Matri.'c Water, RL = 1.0 rng CaCOiL Titrate samples to pH= 4.50 S.U. Page _1..:::2.=---- Page 3 of_3""'--- Time initiated I Time completed 1----' .. r: l 1trant norma ity an d l

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pH?f Normality Deionized T1trant check. U<'J;;III .... water reference standard ml ml -4.5 s.u. number number L b I t d d a oratory contro s an ar : Reference standard True value Sample number (TV) volume Begin (mg CaC03/L) (ml) ml 100 100 ?A.:; M t. 'k a rrx sp1 e recovery: Reference standard Spike value Sample number (SV) volume Begin (mg CaC03/L) (ml) ml 50 iOO {g .\.p Sample alkalinity (B) (mg CaCO;VL) 1-, S I ample measurements: "'* ** ml (E) End ml End ml 1ro.t Normality (N) of H 2 S0 4 pH Factor or Multiplier

  • (5 ml Na 2 C0 3 x 0.05)/E (Nx 50000)/100 ml sample u ....

-Nx 500 (acceptable range 0.0180-0.0220) lk '? Total Multiplier ml 'LI IO.$ Multiplier 10.5 Total Multiplier ml "* "5 I0.-5" Alkalinity (MY) (mg CaCO:YL) Cf(g Alkalinity (mg CaCOJIL) s D % RS = MV I TV x 100 (acceptable range =90 to 110%) %RPD= {(S-D) /[(S+D)/21} x 100 (acceptable range=+/- 10%) Spike alkalinity (A) (mg CaCOJIL) Measured spike value (MY) % R =MY I SV x 100 MV=A-B (acceptable range (mg CaCOy'L) =75 to 125%) Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (ml! CaCOJIL) () 16Z/I. fb z lO D ! '6.\ le-4 "?.;:t {Yi D2 ll .0 J 6Z.m ()r)\ 2tt :'-1-I !f t;J.' &q z_oL tiD O'D\ kl/5 1.1 lz 1 O"JDl.ll.t> 3 C02 q z (2." 1 D'ib£.12. UD002. :..-L11 .:)-:J-61o'JD7.11 Jo. I zs 3ttu 41.-:} 3.\ ILl' l?.o 0462/D,() i \ 2. 41.'1-45.0 33 JA' DqD21l .J 3 . I J.f-5.0 "-llri.1 02.-lQ.()q S/}liS W too e,Ji£ '6q. f 98 Reviewed by: Date reviewed: I OZ-1'5 -o'1 I I I I I I I I I I I I I I I I I I rage 68 0

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Analyst Date analyzed . 0'5 . cq T. itrant normality and multiplier determination: Total Hardness (SM 2340 C) RL = 1.0 mg CaC0 3/L Page t..f.]= Page of_,.;l.=-- Time initiated 11-..;.I.:.D..:::O..;.lP ____ --t Time completed 11 1 5"' .______:::..._ ___ ___. Titrant* Normality check Begin End Total Normality (N) of EDT A pH Factor or Multiplier reference standard ml ml ml = 0.2fE = (N x 50000)/ 50 ml sample number number (E) (acceptable range= 0.0180 -0.0220) =Nx 1000 TN'SS 6--::Jl o.o IO.D [0.0 D.o.Qo cQo .o Lb I d d a oratory contro stan ar : Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaCOJIL) (ml) ml ml ml =90 to 110%) X t.SSS (Qb 4> 40 50 lD.o .;?.o J-fo.o c,olcr Begin End ml ml 1.;u) n:o s IDO _L .L D 11-'ru . 1-5 1-J-5 CJo M t. 'k .;/1.'5 o\lu ,J o/o.o q2. . .;;tatlo a rtx spt e recovery: Reference s'tandard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (mg CaC03/L) (ml) ml ml ml :r: t>J'O s l.Q 5le 4D loo ,;j'3.0 tu.5 C}o,O 130 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (m2 CaCOJIL) = 75 to 125%) CJJ. 3'6 Cl'SO'Lo s l amp.e measurements: Sample volume Begin End Total *Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOJIL) TV=ND Blank :5P .;7t>.O (should be= 0 mg CaCO,/L) fG.Oest.. on.o o.o "->0 1'>2

  • bS *Cf\ 6 (Y)tls rlw J.Y>.o 139..5 1-t.S qi:) _b_ '1. -D4 *DC\ .J &l.5' ?;?.o 4.5 l1o 02-0\-0Ci SsW :n.o 39.1 ll .t.+;l OI.Z'&-0'1 mHs .. u.o 4.-:t-C\1.)-

43$ '4-.S 90 {) 1-2.1 -cctB o.o --t.cs J.t.S qo A . 4-.S' <1.1 I -A-J.. v Dt2 Ol-O.b-a4A . cu 4$ ClLP _.._ 16.£1 V1.'1--5$ --v Note: If >!Sml of titrant is used, sample must be diluted. Reviewed by: Date reviewed I 02-D6-oC) 1()205.01 Page 6

  • 9 ;@) , ______ _ ,sf;! Environmental Testing Solutions.

Inc. Analyst Date analyzed OOl. _ c:fl Total Hardness (SM 2340 C) RL = 1.0 mg CaC0 3/L Page ....f6_;"""-- Page CJ-of----"",;1._,___ Time initiated I ==-==== ._ Time completed '-------.....J T. r itrant norma 'tty and multiplier determination: Titrant Normality check _li'.nrl Total Normality (N) ofEDTA pH Factor or Multiplier .... reference standard ml ml ml , 02/E (Nx 50000)/ 50 ml sample number number (E) (acceptable range= 0.0180-O.u.:.:u Nx 1000 L aboratory control standard: Reference standard True value Sample Hardness (MY) % RS=MV /TV X 100 number (TV) volume Begin End Total Multiplier (mg CaCOYL) (acceptable range (mgCaCOYL) {ml) ml ml ml =90 to 110%) :r )0 40 50 I 'l :).-I.Cf C)s-o?.o Hardness %RPD= Multiplier {mg CaCOJIL) {(S-D) /((S+D)/2]} X 100 2.o.t s J_ Duplicate (B) D J .5 Mi . 'k atrrx sp1 e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mgCaCO)!L) (mg CaCOJIL) (ml) ml ml ml ;:c wss (R 1--{o 5D ;?4.1 4.3 .;;>o .o Sample hardness (B) Measured spike value (MV) % R=MV /SV X 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%) '1C:St:>Lo s l amp. e measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO:JL) TV Rl::mlr I... (should be= 0 mg CaC0 3/L) I .ot.o hTA R.\JiJ) joo

3o.o l.lP 2o.o Jo &H-(p_tt l-5) lP'-fo u_ oqo '1.. o *(")'-' 'rf{A S)Jt: PO\....I{j\.ti\i.

10 o.o (p.t.j-\Q.tf I(!=;) bt1ol aq.o l Scu::h.J/l CJ loo 5/5 /10 ft:N.uxn--;{ l 1'3. ?> J.j.L}-

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_3. \o -:/;1.. b'11J2o+.oz... 3 h ..14.1 lon-.5" -( o& ./ -..... -ot'9H Note: If >15m! oftitrant is used, sample must be diluted. Reviewed by: ltll:..V'-- L-'-'-----.....J Date reviewed I I I I I I I I I I I I I I I I I Page _-4..:._'1-=---- Page --'-_of 3 {) Environmental Testing Sclutlons, Inc. Total Hardness (SM 2340 C) RL = 1. 0 mg CaC0 3/L Analyst I J!,<;(., Date analyzed t-DJ.-=-""',:::.. 5 ___ 0_4_--t ztrant normality and multiplier determination: Titrant Normality check Begin End Total reference standard ml ml ml number number (E) !N'SS 5-:::t \ 0.0 Jo.o 10.0 L b d a oratory control stan ard: Reference standard True value Sample number (TV) *volume Begin End (mg CaCOJIL) (ml) ml ml rt..>sS 40 50 iu.o ld..O Begin End ml ml lv.Cf Duplicate (B) .l-f!Q, q Matrix spike recovery: lYH S 0'1-*\ Reference standard Spike value Sample number (SV) volume Begin End (mg CaCOJIL) (ml) ml ml £.,.. J.fo 5o altO Time initiated f--...:...:.;...:;:;:..=.------t Time completed lll-1 0 __ ___...__, Normality (N) of EDT A pH Factor or Multiplier = 0.2/E = (N x 50000)/ 50 ml sample (acceptable range= 0.0180-0.0220) = Nx 1000 0 .0.:{00 ;(0.0 Hardness (MY) % RS =MY I TV x 100 Total Multiplier (mg CaCOJIL) (acceptable range ml =90 to 110%) J?,O 4o.D loo-:TJ,o Hardness %RPD= Total Multiplier (mg CaC(h/L) {(S. D) /[(S+D)/2]} X 100 ml s qi 4.1-l-D C/4 Spike hardness (A) Total Multiplier (mg CaCOJIL) ml (p .J.t Jo.o f3D Sample hardness (B) Measured spike value (MV) %R=MV /SVx 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) '3'3 ;{J. loSe-to s l ample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC03/L) TV=ND Blank (should be= 0 mg CaC03/L) so tvO 1:12() !..;(11.\/J

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(;, -s.u.J 1-h.O 4t3 ()2.-0t-a:f m'+s \4z1J 3o.r+ 35,0 4-l-:> '12.. 02--H). V1 (\-.J. 3$.0 4-.rJ qo b ffilts ll\J I .q.o!t,O 4.<;" Qo 01--o+uCJ I z .HJ+.o l.r.tt6::l-4.1-qlf Ol-IIJ*OCtl3 J ..3 2.J.S' ..tk5 cto oCt a ?II J I lJPt /{IF flo\'-'N' I 4.':f "1-.l& 3.\ b) 12.0 *:::-\Zy ... -0'llnlt.t6 { J l.o ) t;n,OAA R ll/tll '1-.\o £>.to I Page 70 of 98

  • Note: If >15m! of titrant is used, sample must be diluted. Reviewed by: IL......:.¥l_b-

____ __, Date reviewed I 02-15-dj

  • * '. "' <..t/ tJ{'l !1>-------

_.J Environmental Testing SolutiOns, Inc. Analyst I '6S<... Date analyzed _ l5. D'J itrant normality and multiplier determination: ,. Titrant Normality check Begin End Total refereil"-' ml ml ml number number L b I d d a oratory contro stan ar : Reference standard True value Sample number (TV) volume Begin End (mg CaC031'L) (ml) ml ml Xt-.:6c; olP 40 50 co ,{p 1{).'1* Sample volume Begin End ml ml -SqtJ \t> :-:} 15.0 Duplicate (B) 15.0 tCi;7.. M, t. 'k a TIX spt e recovery: Reference standard Spike valu.e Sample number (SV) volume Begin End (mg CaC031'L) (ml) ml ml :l:i'Y5S.l.il5LP .t..to 5o 15.0 Page S:> Page of 3 -=--Normality (N) of EDTA pH Factor or Multiplier . 0.2/E (N x 50000)/ 50 ml sample I acceptable range 0.0180-0.0220) Nx 1000 .... Hardness (MV) % RS= MV /TV x 100 Total Multiplier (mg CaC03/L) (acceptable range ml = 90 to 110%) ID501.o Hardness %RPD= Total Multiplier (mg CaC03/L) {(S-D) f((S+D)/2]) x 100 ml s SIP j_ D 1-j.,'}_ 84 Spike hardness (A) Total Multiplier (mg CaC031'L) ml Lc>.l 1'20 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaC031'L) = 75 to 125%) CC'f 3l.? qoc-to S I ample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC03/L} TV ND Blank " o1o211. H lvA-SQ,v 1-!5b cll.l '-f*.l Q2... 65 I 3 'Bic CY1 b'2 ()tj' . oz.. -rJA-.SQtJ I Jf ) 2J+.l qz.. 000211.1-;'" I z. '?f.J..l 0 '10 2-\ 3. D l..eo J 3 . 3<l-:z. 4.2.. fN bl) o*z oli. D 1 5DIV o..\1 l 4?, 8l.? I 2.. 0.0 .1.12 I

  • 8<o 0"JD7 (3,0') I ... 3 H-.3 '05 1{.1._. 84 0 lj 02 (}ti. 0 2.. IvA 5& \.) tuivu ' .J. B.5 1).$ 4.:) -\?" Page 71 of 9R . Note: if >15m! of titrant is used, sample must be diluted. Reviewed by: Date reviewed I 16'-0't Page -=5:....:.1

__ Page 3 .,) Environmental Testing Solutions. Inc. Analyst I e,SL.-Date analyzed 1--"W.=* -, Titrant normality and multiplier determination: Titrant Normality check Begin End Total "Normality (N) of EDTA pH Factor or Multiplier refere..-a.. ohnilar-d ml ml ml 0.2/E (N x 50000)/50 ml sample number number lE} '0.0180. 0.0220) Nx 1000 N L aboratorv control standard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaC03/L) (ml) ml ml ml =90 to 110%) 40 50 l1H1 .Q 1-fc:;\ Jo5VZ.C Hardness %RPD= Total Multiplier (mg CaCOJIL) {(S-D) /[(S+D)/2]} x 100 ml ;f.?> <Jo.o s 8<; j Duplicate (B) 4.'2.. J_ D ltt.z. Btf Mi lx ik atr sp, e recovery: Reference standard Spike value Sample Spike (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (m2 CaCOJIL) (ml) ml ml ml ]:NSS(e'Olo 4o 5t> j{.j. 2-t.v.2. Olo.o I Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%) COY. 3lP Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multi}>lier lm11. CaCOJIL) TV=ND Blank ll'-\SfiUUlU DC 00b21:3 Dla -r\ 1 A--2J..3:l 10Hvruv 3 .!:)0 c)S.l.J. .l-t-.'2-cQb.O ()0()211. 0 l e:R.r0 -001 ?,2.\P {1.. IZ.O br1D/.J7. bZ \k(X)} 62_.(1 133.4? l.o tt... 40 lY1DlJ Z .b3 {J)Z lo 31-V q_, 9"'-C\tO 6"Jb 1-l l.D"I ... l)Otoz. 1.0 3l.(p cto ,........ f-" .,...---v -I Page 72 ofJo{t If >15m! of titrant is used, sample must be diluted. Reviewed by: Date revtewed Page 73 of98 Sequoyah Nuclear Plant Biomonitoring February 10 -17, 2009 Appendix D Reference Toxicant Test and Control Chart Page * * * * --u -Ill N u "'CC I r-Pimephales promelas Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc. Environmental Testing Solutions, Inc. USEPA Control Limits (+/- 2 Standard Deviations) 1.0 0.8 0.6 0.4 1.2 USEPA Warning and Control Limits (75th and 9dh Percentile CVs) 1.0 ***** *-**--* ----*-----*----*----**-**-* .--* 0.8 0.6 0.4 -**-*-*-*-----*-*-**----*-**-**-

1.2 Laboratory Warning and Control Limits (1 dh and 25 1 h Percentile CVs) 1.0 0.8 ******** ..... ******* 0.6 -------**-**-

0.4 Test date 7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC 25) Warning Limits (mean IC 25 +/- S AIO or S A 75) Control Limits (mean IC 25 +/- SA.25' SA 90 , or 2 Standard Deviations) '"0 (Jel (';) ........ U1 0 ..... \8 ' * * @ ET Environmental Testing Solutions, Inc. Test number Test date 7-dayiC,. (g!LKCI) CT (g!LKCI) 1 2 4 5 6 7 8 9 10 II 12 13 14 15 16 17 18 19 20 04-15..08 05..06-08 05-20..08 06-03..08 06-10..08 08-05..08 08-12..08 09..09-08 09-11-08 10..07..08 10-14..08 10-28-08 11-04-08 12..09-08 01-06-09 01-13-09 01-15-09 01-21-09 02-03-09 02-10..09 0.60 0.62 0.66 0.64 0.76 0.69 0.76 0.79 0.59 0.57 0.63 0.79 0.67 0.69 0.76 0.82 0.77 0.78 0.79 0.83 0.61 0.63 0.63 0.65 0.66 0.67 0.69 0.68 0.67 0.66 0.67 0.67 0.68 0.68 0.69 0.69 0.70 0.70 0.71 s 0.01 0.03 O.o2 0.06 0.06 0.06 0.07 0.08 0.08 0.07 0.08 0.08 0.07 0.07 0.08 0.08 0.08 0.08 0.08 State and USEPA Control Limits CT-2S CT+2S 0.59 0.57 0.58 0.53 0.55 0.55 0.55 0.53 0.51 0.51 0.52 0.52 0.53 0.53 0.53 0.54 0.54 0.54 0.55 0.63 0.68 0.68 0.78 0.77 0.80 0.83 0.83 0.82 0.81 0.83 0.83 0.82 0.83 0.85 0.85 0.86 0.86 0.88 Pimephales promelas Chronic Reference Toxicant Control Chart SA. II 0.07 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.09 Laboratory Warning Limits CT-SA.Jo CT + SA.IO 0.54 0.55 0.55 0.58 0.58 0.59 0.61 0.60 0.59 0.58 0.59 0.59 0.59 0.60 0.61 0.61 0.62 0.62 0.62 0.68 0.70 0.70 0.73 0.74 0.75 0.77 0.76 0.75 0.74 0.76 0.76 0.76 0.76 0.77 0.78 0.78 0.79 0.80 SA.2S 0.13 0.13 0.13 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.15 0.15 0.15 0.15 Laboratory Control Limits CT-SA.>S CT + SA.2S 0.48 0.49 0.50 0.52 0.52 0.53 0.54 0.54 0.53 0.52 0.53 0.53 0.53 0.54 0.54 0.55 0.55 0.56 0.56 0.74 0.76 0.76 0.79 0.80 0.82 0.83 0.82 0.81 0.80 0.82 0.82 0.82 0.82 0.83 0.84 0.85 0.85 0.86 SA.7S 0.23 0.24 0.24 0.25 0.25 0.26 0.26 0.26 0.25 0.25 0.26 0.26 0.26 0.26 0.26 0.26 0.27 0.27 0.27 USEPA Warning Limits CT-SA.75 CT + SA.75 0.38 0.39 0.39 0.41 0.41 0.42 0.43 0.42 0.41 0.41 0.42 0.42 0.42 0.42 0.43 0.43 0.43 0.44 0.44 0.84 0.86 0.87 0.90 0.91 0.93 0.95 0.94 0.92 0.92 0.93 0.93 0.93 0.94 0.95 0.96 0.97 0.97 0.98 Note: 1-d IC,. = 7-day 25% inhibition concentration. An estimation of tbe concentration of potassiwn chloride that would cause a 25% reduction in Pimephales growth for the test population. CT = Central tendency (mean IC..S). S = Standard deviation of the IC 25 values. Laboratory Control and Warning Limits sA ... 0.27 0.28 0.28 0.29 0.30 0.30 0.31 0.30 0.30 0.30 0.30 0.30 0.30 0.31 0.31 0.31 0.31 0.32 0.32 USEPA Control Limits CV CT-SA.,o CT + Suo 0.34 0.34 0.35 0.36 0.36 0.37 0.38 0.37 0.37 0.37 0.37 037 0.37 0.37 0.38 0.38 0.38 0.39 0.39 0.88 0.91 0.91 0.95 0.96 0.98 1.00 0.98 0.97 0.96 0.98 0.98 0.98 0.99 100 101 101 102 103 0.01 0.04 0.04 0.09 0.08 0.09 0.10 O.ll 0.12 0.11 0.12 0.11 0.11 0.11 0.11 0.11 0.11 0.11 0.12 Laboratory control and warning limits were established using the standard deviation of the IC 25 values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. Suo= Standard deviation corresponding to the lOth percentile CV. (SA 10 = 0.12) SA.,.= Standard deviation corresponding to the 25th percentile CV. (SA.25 = 0.21) USEPA Control and Warning Limits sA.,= Standard deviation corresponding to the 75th percentile cv. ( SA7S = 0.38) SA.9 o = Standard deviation corresponding to the 90th percentile CV. (SA.90 = 0.45) CV = Coefficient of variation of the IC 25 values. USEPA 2000. Understonding and A<:counting fur Method Voriability in Wbole Effluent Toxicity Applications Under tbe National PolluW!t Discharge Eliminalion Program. EPA-833-R.(){)-003. US Envirurunental Protection Agency, Cincinnati, OH. Organjsms obtained from A quat ox. Inc. atox02*1 0-09

  • * *
  • ETS Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Pimephales promelas Chronic Reference Toxicant Data Test number Test date Control Control Mean CT cv CT Survival Growth (%) (mg!Iarvae) for Control Growth (%) for Control (mg!Iarvae)

Growth cv (%) 04-15-08 100 0.898 9.1 2 05-06-08 100 0.857 0.877 4.4 6.7 3 05-20-08 100 0.844 0.866 19.2 10.9 4 06-03-08 100 0.918 0.879 6.1 9.7 5 06-10-08 97.5 0.724 0.848 10.9 9.9 6 08-05-08 100 0.854 0.849 15.3 10.8 7 08-12-08 100 0.674 0.824 5.3 10.0 8 09-09-08 100 0.710 0.810 11.1 10.2 9 09-11-08 100 0.824 0.811 11.1 10.3 10 10-07-08 100 0.788 0.809 1.8 9.4 11 10-14-08 100 0.740 0.803 3.2 8.9 12 10-28-08 100 0.586 0.784 6.5 8.7 13 11-04-08 97.5 0.643 0.774 2.5 8.2 14 12-09-08 100 0.522 0.756 2.4 7.8 15 01-06-09 100 0.644 0.748 3.2 7.5 16 01-13-09 97.5 0.556 0.736 9.7 7.6 17 01-15-09 100 0.707 0.734 6.5 7.5 18 01-21-09 97.5 0.594 0.727 4.9 7.4 19 02-03-09 100 0.671 0.724 8.4 7.4 20 02-10-09 100 0.591 0.717 5.1 7.3 Note: CV = Coefficient of variation for control growth. Lower CV bound detennined by US EPA (1Oth percentile)= 3.5%. Upper CV bound determined by USEPA (90th percentile)= 200/o MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference MSD PMSD CT (%) forPMSD(%) 0.08 9.1 0.16 18.2 13.7 0.16 18.8 15.4 0.09 9.4 13.9 0.11 15.7 14.3 0.13 15.0 14.4 0.07 9.8 13.7 0.12 17.3 14.2 0.11 12.9 14.0 0.10 12.9 13.9 0.05 7.2 13.3 0.10 17.8 13.7 0.12 18.7 14.1 0.05 10.0 13.8 0.09 13.3 13.7 0.06 11.2 13.6 0.09 13.3 13.6 0.07 11.5 13.5 0.06 9.6 13.3 0.09 15.7 13.4 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10m percentile)

12%. Upper PMSD bound determined by USEPA (90th percentile)

30%. CT =Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200la, 200Ib. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtained from Aquatox, Inc. atox02-l 0-09 Page 76 of98 .Q ...... Q a.. -Q a.. ...... = Q u --= Q .... ..... c.s *c = > c.,., Q ..... 5 *-c:J e Q u * * * * ,_) c.s £: c.s --= .... ...... .... = . ... -bJ) s '-' -= ..... -Q a.. ..... = Q u a.. Pimephales promelas Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Organism Source: Aquatox, Inc. 1.00 --0.75 -----------0.50 0.25 *-*-*--*-* US EPA Acceptance Criteria(> 0.25 mg per surviving larvae) 30 **-* -*--*-*-**-*-*-**-**-**-**-**-**-**-**-**- --*-*---*-*-** Kentucky Acceptance Limit(< 30.0%) 20 10 0 30 USEPA Upper PMSD Bound (90th percentile< 30.0%) 10 0

A\%
,\'I'll ,..\'!% (l A\'b ll.-\'!'ll

%,\'!% ll.-\'1% b,\'!'l y\)'l v\'!'l :;-\'!'l Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) I I I I I * " ;;"!* I ' I . ,. ... il Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Page 1 of 5 PpKClCR Test Number: 110 Dilution preparation information: Comments: KCl CHM number: (,\-\ 11 t.t 01-Stock preparation: 50 g KCIIL: Dissolve 50 g KCI in 1-L Deionized water Dilution prep (mg!L) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test orKanism information: Test information: Organism age: 1'\. So l-tt\uitS OL.b Randomizing template: . f' fL .e_ Date and times organisms 01.* 0 "'. 04\ ll.oO Incubator number and 3E: were born between: shelf location: Organism source: A"TM e,A\(.1'\ ll'l* ri\*CA Artemia lot number: ehtw/10 Transfer bowl information: pH= SU Temperature= oc Total drying time: 1.b?... '2"f**3 Date I Time in: 6.1.*i1*1l'i lfi_Q Average transfer volume: D. 4 .-J).. Date I Time out: uv1Ht\ 1ILQ_ Oven temperature: too* c. Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, MHS batch used Analyst feeding feeding time time 0 2 3 4 I I I I I I I I I --I -79 of98 Page 2 of5 Species: Pimephales promelas PpKCICR Test Number: 11 0 Survival and Growth Data Day Control 450 600 KCl/L A B c D E F G H I J K L 0 /0 10 IT; IO /Q /fJ IO to 10 10 10 10 1 IO 10 (0 '0 IO 10 10 10 ID 10 (0 /0 2 IO 10 10 10 10 /0 10 10 10 10 10 10 3 /Q ID IO 10 /() tO IO I() 10 /Q 10 I\:) 4 to 10 to to IO 10 tO lO (() 10 10 I() 5 /c to I 0 lQ to (0 lO 1() ID /Q It;, 10 6 /0 /0 /0 10 IO lb IO IO /0 10 10 7 -,9-\ q {0 /0 /0 /CJ 10 10 (() 10 10 tO 10 A= Pan weight {mg) Tray color LM y-e. d lt{.oq IS.03 ll\.1.\lo 13.S3 1"\.\t 13 .l.f 'l Analyst: l Date: ()1..-\0-0"1. B-Pan + Larvae weight ll\.5"8 (mg) ;ll-15' Jq, 11.'\l }q." ;)b.;).g 18.1.(' ;)1).1& Itt. ;)14 .).0.3& 1'\-bq ,q,6q Date: Ol.*l! -I)'\ C-Larvae weight (mg) =A-B s.tl'\ "'* 1'2. $.'1\ 1-t,'\() s.u. ._.o'-t 5*'-12. c..() 2.. Weight per initial number ft,rv .>. ;:,.."V t)'V ,o oflarvae (mg) tl' (1<..0 0 = C I Initial number oflarvae v t; '4> .). ta \.PC I? \II c., \I " o* o' o' o* t>' o* o* I)' o* t)' !;)' ()' .Average Percent weight per reduction 4.17 .. initial from control o.sq \ number of (%) larvae (ml!) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: L Comments: I I I I I I * * "* $ J.t .) E-nv-iron-me-nu-IT .. -tln-g S-olu-tlo-n*,-lnc. Species: Pimephales promelas Day 0 1 to ID 2 lb (() 3 to IO 4 '1 IC 5 q lO 6 ({) 7 & IO A= Pan weight (mg) Tray color code:: red 13. 'i't I'! .ll.f Analyst: Date: B = Pan + Larvae weight (mg) I,, Analyst: Date: C =Larvae weight (mg) =A-B "i.OD '$. Weight per initial number of larvae (mg) = C I Initial number of larvae o* Percent reduction from control a .s-z..c (%) ID IO 10 10 lO /0 I 0 10 10 10 10 10 (0 tO tO li$. S(p $.11.\ \'2... Page 3 of 5 PpKCICR TestNumber: 110 /() q'rA loi(J.. &tJ.. lo '1 lD 1,J. (. t.\ fo 1 lo 1..\ q (, l (, y to 1 \,. 4 .3 s 6 "' '-I A 1.. 3 1.3 ..3to .os lt.3() 1"\.15 1y.s-3 JS,!l, 14.tW lt+i l.o.\'% 1.11 \. 2..11.\ -z.o3 ,-" 1,(/. 11,7 " " 'i::f \)' t;)" o* c* t)" cr ()' .,3t.,.&7 .. Q,\1q

  • :comment codes: c =clear, d =dead, fg = fungus, k =killed, m =missing, sk = sick, sm = unusually small, * . == 1;IDUSually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed:

(JCI (t> 00 ..... 0 ..... \C 00 * * * * "0:1:!? 0 Environmental Testing Solutions, Inc. coo...,tratioa CroWL llcpli-KO) A B Control c D E 450 F G H [ J 600 K L M N 750 0 p 0 R 900 s T u v 1050 w X Dunnett's MSD value: PMSD: Ialtialaullll><rof 1 ...... 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 0.0926 !5.7 Fiaalaumber of larvae 10 10 10 10 10 10 9 10 10 10 10 10 8 10 10 10 7 5 6 6 2 3 3 3 A Pu (mgj 14.09 15.03 13.94 13.79 14.26 14.46 13.83 14.12 13.87 14.34 13.49 14.19 13.44 14.14 13.86 13.46 14.98 14.06 14.93 15.51 13.36 14.08 12.30 14.75 MSD= PMSD= Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses B = Pas + Larv1e La.., .. ll'<igjlt (rna) Wdglu I So"'ivi*K Mcuwciglll/ Coeftlc:ie*t ofvariadoo Wcigllt /laitial aumbc:r wcight (mg) *A-B ......... olWv&e (ma) Su..,..iviag umber of ..... oflarne (mgj larvae (mg) __ ._..,.._ .. ,(%) 19.58 5.49 0.549 0.549 21.15 6.12 0.612 0.591 5.1 0.612 19.85 5.91 0.591 0.591 19.92 6.13 0.613 0.613 19.16 4.90 0.490 0.490 20.28 5.82 0.582 0.548 9.9 0.582 18.46 4.63 0.514 0.463 20.16 6.04 0.604 0.604 19.29 5.42 0.542 0.542 20.36 6.02 0.602 0.564 4.7 0.602 19.09 5.60 0.560 0.560 19.69 5.50 0.550 0.550 17.44 4.00 0.500 0.400 19.87 5.73 0.573 0.545 6.5 0.573 19.18 5.32 0.532 0.532 19.20 5.74 0.574 0.574 19.11 4.13 0.590 0.413 17.34 3.28 0.656 0.625 8.3 0.328 19.02 4.09 0.682 0.409 18.95 3.44 0.573 0.344 14.53 1.17 0.585 0.117 15.89 1.81 0.603 0.645 9.4 0.181 14.44 2.14 0.713 0.214 16.78 2.03 0.677 0.203 Minimum Significant Difference Percent Minimum Significant Difference Test number: PpKCICR 11170 Test dates: February 16-17, 2009 '-""I Mcln sunival Meaa Might/ laitlal Codli<ieor ol Perceat red.c1ioa rrom (Y*) **mber of larvae var:ialiol t*!.) COitroJ W*) (mg) 100.0 0.591 5.1 Not applicable 97.5 0.535 12.9 9.6 100.0 0.564 4.7 4.7 95.0 0.520 15.8 12.1 60.0 0.374 11.7 36.8 27.5 0.179 24.3 69.8 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. LowerPMSD bound determined by USEPA (loth percentile)

12%. Upper PMSD bound deternrined by USEPA (9oth percentile)

30"/o. Lower and upper PMSD bounds were detennined from the I oth and 9oth percentile. respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEP A, 200 1a; USEP A, 200 I b). USEPA 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods. Volwnes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Ageocy, Cincinnati, OH. Organisms obtained/rom Aquatox. Inc. atox02-l 0-09 ""= (J(l ('tl QCI N 0 ...., \C QCI .ETS * * * * ":* *'. :;, *, . . ..* ..*.. , ::1,;._ . Statistical Analyses Environmental Testing Solutions, Inc. Larval Fish Growth and Survival Test-7 Day Survival Start Date: 2/10/2009 TestiD: PpKClCR Sample ID: REF-Ref Toxicant End Date: 2/17/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR.-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mgiL 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 0.9000 1.0000 600 1.0000 1.0000 1.0000 1.0000 750 0.8000 1.0000 1.0000 1.0000 900 0.7000 0.5000 0.6000 0.6000 1050 0.2000 0.3000 0.3000 0.3000 Transform: Arcsin Square Root Rank I-Tailed Number Total Number Conc-mgiL Mean N-Mean Mean Min Max CV% N Sl!f11__ __ Critical Resp D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 450 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 600 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 750 0.9500 0.9500 1.3358 J.l071 1.4120 Il.411 4 *900 o.6000 0.6000 0.8872 0.7854 0.9912 9.469 4

  • 1050 0.2750 0.2750 0.5506 0.4636 0.5796 10.532 4 Auxiliary Tests Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) Equality of variance cannot be confirmed Hypothesis Test (1-tail, 0.05) Steel's Many-One Rank Test Treatments vs D-Control Trim Level EC50 0.0% 5.0% 10.0% 20.0% Auto-27.5%

942.95 NOEC 750 95%CL 874.89 1016.31 Organisms oQ.tained from A quat ox, Inc. LOEC 900 Statistic 0.83137703 ChV TU 821.583836 Trinuned Speannan*Karber 16.00 18.00 16.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Critical 0.884 0 1 0 2 16 29 40 40 40 40 40 40 Skew Kurt -1.5887618 3.41097967 atox02-I 0-09 00 Q ....,, \0 00 !ETS Statistical Analyses Environmental Tasting Solutions, Inc. Larval Fish Growth and Survival Test-7 Dar Growth Start Date: 2/10/2009 TestiD: PpKCICR SampleiD: End Date: 2/17/2009 LabiD: ETS-Envir. Testing Sol. Sample Type: Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: Comments: Conc-mg/L l 2 3 4 D-Control 0.5490 0.6120 0.5910 0.6130 450 0.4900 0.5820 0.4630 0.6040 600 0.5420 0.6020 0.5600 0.5500 750 OAOOO 0.5730 0.5320 0.5740 900 0.4130 0.3280 0.4090 0.3440 1050 0.1170 0.1810 0.2140 0.2030 Transform: Untransformed Mean N-Mean Mean Min Max CV% N t-Stat D-Control 0.5913 1.0000 0.5913 0.5490 0.6130 5.063 4 450 0.5348 0.9044 0.5348 0.4630 0.6040 12.856 4 1.397 600 0.5635 0.9531 0.5635 0.5420 0.6020 4.739 4 0.686 750 0.5198 0.8791 0.5198 OAOOO 0.5740 15.815 4 1.767 900 0.3735 0.6317 0.3735 0.3280 0.4130 11.733 4 1050 0.1788 0.3023 0.1788 0.1170 0.2140 24.276 4 Auxiliary Tests Statistic Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94821143 Bartlett's Test indicates equal = 0.21) 4.54906893 Hypothesis Test (1-tail, 0.05). NOEC ___ ChV TU MSDu MS.Qp_ Dunnett's Test 750 >750 0.09263803 0.15668167 Treatments vs D-Control Linear Inte!pOlation (200 Resamples) Point mg/L SD 95%CL(Ex2l Skew IC05* 315.80 185.01 86.12 1024.71 0.4865 IC10 686.81 132.81 139.11 839.84 -1.3149 IC15 767.63 52.55 575.49 832.99 -3.1043 IC20 797.95 33.93 638.23 857.16 -1.6553 IC25 828.27 28.80 688.64 881.73 -1.5389 IC40 914.44 14.68 866.48 950.31 -0.2463 IC50 959.98 11.87 922.86 990.27 -0.1105

  • indicates IC estimate less than the lowest concentration Organisms obtdln(l.afrom Aquatox, Inc. REF-Ref Toxicant KCL-Potassiwn chloride PP-Pirnephales promelas 1-Tailed Isotonic Critical MSD Mean N-Mean 0.5913 1.0000 2.290 0.0926 0.5491 0.9288 2.290 0.0926 0.5491 0.9288 2.290 0.0926 0.5198 0.8791 0.3735 0.6317 0.1788 0.3023 Critical Skew Kurt 0.844 -0.8175786 0.46062928 11.3448668 MSB MSE F-Prob df 0.0040134 0.00327294 0.34279487 3, 12 atox02-l 0-09
  • ;t >'II --;J Species: Pimephales promelas 1050 mg KCI/L STOCK Page 4 of5 PpKClCR Test Number: 110
  • Er * * *

-' __ ... Page 5 of5 .,} EnvlronmenlatTesting Solutions, Inc. Species: Pimephales promelas PpKClCR Test Number: /1 0

  • * * * *8 . .) C' u z -on M u l-ot "0 I r-Ceriodaphnia duhia Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc. 1.14 USEPA Control Limits (+/- 2 Standard Deviations) 1.12 1.10 ..... 1.08 ---1.06 --1.04 1.02 2.5 USEPA Warning and Control Limits (75 1 h and 9dh Percentile CVs) 2.0 1.5 1.0
  • S
  • __. * * * * * -=-=-=
  • a =====* S *
  • S .-== ..... 0.5 1.4 1.3 Laboratory Warning and Control Limits (1 dh and 25 1 h Percentile CVs) 1.2 1.1 1.0 0.9 Test date 7-day IC 25 = 25% inhibition concentration.

An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. --. Central Tendency (mean IC 25) --**-WamingLimits(meaniC 25+/-SA.10 or SA.75) p ge 8-6 of 9 S Control Limits (mean IC 25 +/- S A25' S A.90 , or 2 Standard Deviations) (JQ QO ....... Q ""'l *

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  • Environmental Testing Solutions, Inc. Test number Test date 7-day IClS CT (giL NaCI) (giL NaCI) 10-02-07 1.06 2 1.06 1.06 3 11-06-07 1.07 1.06 4 12-04-07 1.07 1.07 5 01-08-08 1.09 1.07 6 Ol-08-08 1.09 1.07 7 02-05-08 1.07 1.07 8 03-04-08 1.07 1.07 9 04-08-08 1.07 1.07 10 05-06-08 1.06 1.07 II 06-03-08 1.11 1.07 12 07-08-08 1.07 107 13 08-05-08 1.09 1.08 14 09-09-08 1.10 1.08 15 10-07-08 1.09 1.08 16 11-04-08 1.07 1.08 17 IJ-04-08 1.10 1.08 18 12-02-08 1.06 1.08 19 01-06-()9 1.05 108 20 02-03-09 105 1.08 s 0.00 O.ot 0.01 0.01 0,01 0.01 0.01 0.01 0.01 0.01 O.ot O.Dl 0.02 0.02 0.01 0.02 0.02 0.02 0.02 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart State and USEPA Control Limits SA.1 o CT-2S CT+2S 1.05 1.06 0.08 1.05 1.08 0.09 1.05 1.08 0.09 1.04 110 0.09 1.05 1.10 0.09 1.05 1.10 0.09 1.05 1.10 0.09 105 1.10 0.09 1.05 1.09 0.09 104 1.10 0.09 105 1.10 0.09 1.05 1.11 0.09 1.05 1.11 0.09 1.05 1.11 0.09 105 1.11 0.09 105 1.11 0.09 1.05 1.11 0.09 105 1.11 0.09 1.04 1.11 0.09 Laboratory Warning Limits CT-SA.IO CT + SA.lt 0.97 1.14 0.98 1.15 0.98 1.15 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.17 0.99 1.16 0.99 1.16 0.99 1.16 Laboratory Control Limits S,.,,. CT -SA.lS CT + SA.lS 0.18 0.88 1.24 0.48 0.18 0.88 1.24 0.48 0.18 0.88 1.25 0.48 0.18 0.89 1.25 0.48 0.18 0.89 1.26 0.48 0.18 0.89 1.26 0.48 0.18 0.89 1.26 0.48 0.18 0.89 1.25 0.48 0.18 0.89 1.25 0.48 0.18 0.89 1.26 0.48 0.18 0.89 1.26 0.48 0.18 0.89 1.26 0.48 0.18 0.89 1.26 0.48 0.18 0.89 1.26 0.49 0.18 0.89 1.26 0.48 0.18 0.90 1.26 0.49 0.18 0.89 1.26 0.49 0.18 0.89 1.26 0.48 0.18 0.89 1.26 0.48 USEPA Warning Limits CT-SA., CT + SA.,s 0.58 1.54 0.58 1.54 0.59 1.55 0.59 1.55 0.59 1.56 0.59 1.56 0.59 1.56 0.59 1.56 0.59 1.55 0.59 1.56 0.59 1.56 0.59 1.56 0.59 156 0.59 1.56 0.59 1.56 0.59 1.56 0.59 1.56 0.59 1.56 0.59 1.56 sA. .. 0.66 0.66 0.66 0.66 0.67 0.67 0.67 0.67 0.66 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 Note: 7-d = 7-&y 25% inhibition concentration.

An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC 2 s). S = Standard deviation of the IC, values. USEPA Control Limits cr-S..., 0 cr +sA. .. 0.40 1.72 0.40 1.72 0.41 1.73 0.41 1.73 0.41 1.74 0.41 1.74 0.41 1.74 0.41 1.74 0.41 1.74 0.41 1.74 0.41 1.74 0.41 1.74 0.41 1.74 0.41 1.75 0.41 1.75 0.41 1.75 0.41 1.75 0.41 1.74 0.41 1.74 cv 0.00 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 O.Dl 0.01 0.01 0.01 0.01 0.01 0.01 0.02 Control and Waraing Limits Laboratory control and warning limits were established using the standard deviation of the values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEP A for the test method and endpoint. s,\.10 =Standard deviation corresponding to the 10 111 percentile cv. (SAIO = 0.08) = Standard deviation corresponding to the 25 111 percentile CV. (SA2s = 0.17) USEPA Control and Warning Umits SA.75 = Standard deviation corresponding to the 75fll percentile CV. (SA 75 = 0.45) SA, 0= Standard deviation corresponding to the 90 111 percentile CV. (SA.90 = 0.62) CV = Coefficient of variation of the values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH. 02-03-09

  • Precision of Endpoint Measurements
  • Ceriodaphnia dubia * . Chronic Reference Toxicant Data Environmental Testing Inc. Test Test date Control Control Mean CT MSD PMSD CT number Survival Reproduction CT cv for Control Mean for Control (%) (offspring/female)

Reproduction (%) Reproduction (%) forPMSD (%) (offspring/female) CV(%) 1 10-02-07 100 29.5 9.4 2.4 8.2 2 11-06-07 100 28.0 28.8 6.1 7.7 2.5 8.8 8.5 3 11-06-07 100 30.4 29.3 5.6 7.0 2.2 7.2 8.1 4 12-04-07 100 31.8 29.9 5.5 6.7 2.5 7.9 8.0 5 01-08-08 100 30.3 30.0 6.6 6.6 2.2 7.3 7.9 6 01-08-08 100 31.8 30.3 4.9 6.3 2.4 7.7 7.8 7 02-05-08 100 31.3 30.4 6.4 6.4 2.8 9.1 8.0 8 03-04-08 100 31.5 30.6 8.0 6.6 2.7 8.5 8.1 9 04-08-08 100 29.6 30.5 7.7 6.7 2.6 8.8 8.2 10 05-06-08 100 32.1 30.6 6.3 6.6 2.5 7.9 8.1 11 06-03-08 100 30.5 30.6 7.9 6.8 3.0 9.9 8.3 12 07-08-08 100 30.9 30.6 6.7 6.8 2.4 7.7 8.2 13 08-05-08 100 29.4 30.5 7.9 6.8 2.2 7.6 8.2 14 09-09-08 100 28.3 30.4 8.5 7.0 3.0 10.5 8.4 15 10-07-08 100 32.4 30.5 7.2 7.0 2.7 8.4 8.4 16 11-04-08 100 32.3 30.6 5.1 6.9 2.2 6.8 8.3 17 11-04-08 100 31.5 30.7 6.2 6.8 2.4 7.7 8.2 18 12-02-08 100 31.5 30.7 4.6 6.7 2.5 8.0 8.2 19 01-06-09 100 33.2 30.9 4.0 6.5 2.0 6.1 8.1 20 02-03-09 100 31.4 30.9 6.6 6.5 1.9 6.0 8.0 Note: CV = Coefficient of variation for control reproduction. Lower CV bound determined by USEPA (lOth percentile)= 8.9%. Upper CV bound determined by USEPA (90th percentile)= 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEP A (1Oth percentile)

13%. Upper PMSD bound determined by USEPA (90th percentile)

47%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200la, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. 02-03-09 Page 88 of98 = Q .... .-= "'0 * * *

  • J -Q# -= e Q# = *;:: -c. Q e ... ...... = Q Q -u Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Environmental Testing Solutions, Inc. 40 35 30 25 20 15 **-*-*--*-

Minimum Acceptance Criteria(> 15.0 offspring per surviving female) North Carolina Acceptance Limit ( < 40.0%) Kentucky Acceptance Limit(< 30.0%) USEPA Upper PMSD Bound (90th percentile < 47.0%) 40 30 20 10 \\...\\ \,....\\ Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD) P ge 89 of 98 Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations) lige 90 of98 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia CdNaCLCR #: 85 Stock preparation: 600 9 1491 1488 1500 1500 Daily renewal information: Day Date 0 2 3 4 5 6 7 Control in ormation: % Mortali Mean Offs %CV: 1200 1400 15 18 21 1485 1482 1479 1500 1500 1500 < 24-hours old Test initiation and feeding, renewal and feeding, or termination time Acceptance criteria S20% S:20% Comments: Selenastrum batch: MHSW batch used 15.0 offspring/female <40.0% Analyst Page I of6 91 of98 Page 2 of6 CdNaCLCR #: 8S Concentration: % Mortality: I 07 .. Mean Offspring/Female: 1 ,3\.'-\ 600 mg NCI/L a S . I dR UTVlVa an d t' D t epro uc zon a a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced n () 0 (") 0 0 0 0 0 0 Adult mortality L L L L L L L L L L 2 Young produced 0 0 0 0 0 0 0 0 0 a Adult mortality L L L L L L L L_ L \.... 3 Young produced 0 0 0 a 0 0 0 0 c 0 Adult mortality L L L L L L L L L L 4 Young produced s '-\ l, lo '5 s '-\ '-\ s s Adult mortality L L L L L L L.. L L L 5 Young produced l"Z.. \-z-l\ l"\ \"\ l"t 10 Adult mortality l-L L L L. L L L L L_ 6 Young produced t"-\ IS l'-\ ll.o \'S 1$ 1'-t l.S Adult mortality \._ '--\._ L \,_ '--L \._ L. L 7 Young produced Total young produced ..!..\ 31. 3S o?> 3'2... .3\ 30 Final Adult Mortality L L '-L L L. L l l L. Note: Adult mortality (L-live, D-dead), SB-spht brood (smgle brood spht between two days), CO-carry over (offsprmg carried over with adult during transfer). Concentration: % Mortality: Q7 .. Mean Offspring/Female: 32..0 % Reduction from Control: ---V\7o ,, !"*,. Page 3 of6 Species: Ceriodaphnia dubia 'soo N cl!L S . I dR CdNaCLCR #: & S d t" D t "' mg a urvzva an epro uc zon a a Replicate number . "\ Day 1 2 3 4 5 6 7 8 9 10 1 Young produced () D 0 D 0 0 0 0 C) 0 ' L Adult mortality '-\._ \..._ L \.._. \.._. '-"-L ' 2 Young produced n D 0 0 a 0 0 () a 0 Adult mortality \._ L L L L L L L_ '-\._ 3 Young produced 0 0 0 D 0 a u 0 a 0 Adult mortality L L \..._ . L L L L.. L L. \._ 4 Young produced $ u. s s s 4 '-\ -q \o s Adult mortality L \.... \._ L \_ L.. \_ '-'-'-5 Young produced \?_ \C \1 .. \S -\l\ Il-l n. ,,_ t"t.. Adult mortality '-'--'-L L \... \.... L L L 6 Young produced "' -1'-\ t'-4 '"" l'S. lL.. 1\o Adult mortality \... \_ \._ L '-L \... L-\..... \...... 7 Young produced _, Total young produced "2.& .J.'-\ 32.. .3"1. .3o 3'!> Final Adult Mortality \.._ L L \.... L L.. L. \..... L. '-Note: Adult mortaltty (L-hve, D =dead), SB = spltt brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer). 1000 mg NCI/L a S . l dR urvzva an d t" D t epro uc zon a a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced .0 0 0 () 0 0 0 (\ 0 a Adult mortality \._ L L.. L L -c L L L L 2 Young produced 0 () 0 a 0 0 0 0 0 () Adult mortality L L L L L L L L L L 3 Young produced 0 6 0 0 a 0 -o 0 0 0 Adult mortality '-L L L L L L '-L L 4 Young produced s s to '4 s s s s Adult mortality L L L.. L L. \..... '-'-'--L.. 5 Young produced l\ lO lD 11 .. I l l"L L 1... I"'Z.. Adult mortality L L.. L \...... L L L L \.... \...... 6 Young produced 1'-\ Ll '-z.. 10 tS 17.. IL.. I l Adult mortality '-L L L '--L L L L L 7 Young produced Total young produced 3C "'L$ "l..C\ l.'\ 2.<o 3\ 2'\ '2..<1\ Zi Final Adult Mortality L '-L. L. L \.... L -c.:* '-'-Note: Adult mortality (L"' hve, D "'dead), SB =split brood (single brood split between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: % Mortalitv:

07. Mean Offsoring/Female:

"2&*, %Reduction from Control: g .(,7, 92 of98 I ,, I

  • l I IJ It age 93 of 98 Page 4 of6 Species: Ceriodaphnia dubia CdNaCLCR #: & S 1200 N CIIL mg a S . I dR urvzva an d r* D epro uc zon at a Replicate number , .. **Day 1 2 3 4 5 6 7 8 9 10 o-* 1 Young produced 0 0 0 0 D 0 0 0 0 D Adult mortality L L L '-'-L L '-\-. L 2 Young produced 0 0 a 0 a 0 0 0 0 0 Adult mortality L L l.... L L L '-L \..._ L 3 Young produced D 0 0 0 0 0 0 C) 0 0 Adult mortality

\...... ... Ll-L L. L.. '--L '-L 4 Young produced '-\ s 3 t..t Ll 4 4 Adult mortality L L L \._ '-'-L '-\.._ \...._ 5 Young produced Q s I. \c. 0 s s L... Adult mortality L-'-L '-L L '--'-L. '--6 Young produced 0 0 0 0 0 0 \.o 0 0 5 Adult mortality '-L. \..... L. L L \...... '--L '-7 Young produced Total young produced .3 , R 1(.) t; q , 1 \ Final Adult Mortality L. L-L. '--'--'-L '-\..._ Note: Adult mortaltty (L = hve, D =dead), SB =split brood (smgle brood spltt between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: % Mortality: o1. Mean Offspring/Female: &A % Reduction from Control: "L'J. 1400 mg NCIIL a S . l dR urvzva an d epro uction D t a a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced () 0 0 D 0 0 0 0 n 0 Adult mortality L '-L. L.. L '-'-L '-\.... 2 Young produced 0 0 0 0 0 0 () () a 0 Adult mortality L L '--L-L L L L L l_ 3 Young produced 0 0 0 D 0 0 0 0 0 0 Adult mortality L '-L L L L. '-L L L 4 Young produced 0 1.. '1.. \ 1... D 0 0 -z..... Adult mortality L '-L L L L L '-L L 5 Young produced 3 D a '"L () D 4 ' _Q Adult mortality L L L. L-L '-L. L-L L 6 Young produced D D-0 D 0 0 0 0 0 6 Adult mortality L \.,_ L L L \...... L L. \... L. 7 Young produced Total young produced .:!, 2.. L. .a. "2.. -3 3 4 I l.. Final Adult Mortality L L '--L. '-\...... L. '-'--L. Note: Adult mortahty (L = hve, D =dead), SB =split brood (smgle brood spbt between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: % Mortality: Q1. Mean Offspring/female: '2.*'5 %Reduction from Control: 'Jl,.() d \} '"Q iiQ (D \C ""' s..ET * * ,lf,..'!JW "': 0 Environmental Testing Solutions, Inc. Control ... -* -*-*------* Verification of Ceriodaphnia Reproduction Totals 1000 NaCI/L I.C til Q ......, I.C QO * * *

  • ET Environmental Testing Solutions, Inc. Concentration (mg!L NaCI) I 2 3 Control 31 31 33 600 33 31 32 800 30 28 33 1000 30 31 25 1200 3 7 8 1400 3 2 2 -------------Dunnett's MSD value: 1.876 PMSD: 6.0 Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses 4 30 35 34 29 8 3 MSD= PMSD= C:dN*OCR N85 Test Test datd: February OH9, 2009 / '\/, 1..... ..)J Replicate number Survival Average reproduction Coefficient of Percent reductiou from 5 6 7 8 9 10 ("/o) (offspring/female) variatioa

(%) ooatrol (%) 28 35 30 34 31 31 100 31.4 6.6 Not applicable 29 34 33 32 31 30 100 32.0 5.7 -1.9 31 32 32 31 30 33 100 31.4 5.7 0.0 29 26 31 29 29 28 100 28.7 6.8 8.6 10 9 10 9 9 11 100 8.4 26.4 73.2 2 3 3 4 1 2 100 2.5 34.0 92.0 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (lOth percentile).

13%. Upper PMSD bound determined by USEPA (90th percentile)

47%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data [Tom EPA's WET Interlaboratory Variability Study (USEPA. 200la; USEPA. 2001b). USEP A. 2001 a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01 -004 and EP A-821-B-0 1-005. US Environmental Protection Agency, Cincinnati, OH. ""C I1Q \C 0\ 0 ..... \C 00 .ETS * * * ' .. +/--.. . .:... ,. :f, Testing Solutions. Inc. Start Date: 2/3/2009 End Date: 2/9/2009 Sample Date: Comments: Conc-mg/L 1 2 D-Control 31.000 31.000 600 33.000 31.000 800 30.000 28.000 1000 30.000 31.000 1200 3.000 7.000 1400 3.000 2.000 Cono-mg/L Mean N-Mean D-Control 31.400 1.0000 600 32.000 1.0191 800 31.400 1.0000 *1000 28.700 0.9140 *1200 8.400 0.2675 *1400 2.500 0.0796 Test ID: Lab ID: Protocol: 3 33.000 32.000 33.000 25.000 8.000 2.000 Mean 31.400 32.000 31.400 28.700 8.400 2.500 Statistical Analyses CeriodaEhnia Swvival and R!:Eroduction Test-R!:Eroduction CdNaCICR SampleiD: REF-Ref Toxicant ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia 4 5 6 7 8 9 10 30.000 28.000 35.000 30.000 34.000 31.000 31.000 35.000 29.000 34.000 33.000 32.000 31.000 30.000 34.000 31.000 32.000 32.000 31.000 30.000 33.000 29.000 29.000 26.000 31.000 29.000 29.000 28.000 8.000 10.000 9.000 10.000 9.000 9.000 11.000 3.000 2.000 3.000 3.000 4.000 1.000 2.000 Transfonn: Untransfonned !-Tailed Isotonic Min Max CV% N t-Stat Critical MSD Mean N-Mean 28.000 35.000 6.578 10 31.700 1.0000 29.000 35.000 5.705 10 -0.731 2.287 1.876 31.700 1.0000 28.000 34:.000 5.657 10 0.000 2.287 1.876 31.400 0.9905 25.000 31.000 6.782 10 3.290 2.287 1.876 28.700 0.9054 3.000 11.000 26.442 10 28.029 2.287 1.876 8.400 0.2650 1.000 4.000 33.993 10 35.219 2.287 1.876 2.500 0.0789 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates nonnal distribution (p > 0.01) 0.95103425 1.035 -0.5620922 0.76156552 Bartlett's Test indicates equal variances (p = 0.18) 7.56523657 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 800 1000 894.427191 1.87636754 0.05975693 1771.72 3.36666667 1.7E-44 5, 54 Treatments vs D-Control Linear Interpolation (200 Resamples) Point m!i!: SD 95%CL Skew IC05 895.185185 39.6216408 798.428309 967.186364 -0.4820 IC10 1001.67488 24.6399956 926.84517 1012.24105 -1.3664 IC15 1017.29064 6.93088301 1003.19782 1027.02713 -1.7330 IC20 1032.9064 5.81256688 1019.78841 1041.72271 -0.5668 IC25 1048.52217 5.49826611 1036.37899 1057.13228 -0.5823 IC40 1095.36946 4.95417378 1084.62237 1102.73588 -0.5583 IC50 1126.60099 4.99006093 1115.31017 1133.67544 -0.4842 02-03-09 Page 5 of6 Species: Ceriodaphnia dubia CdNaCLCR #: &S CONTROL 600 mg NaCI!L 800 mg NaCI!L 1000 mg NaCI/L 1200 mg NaCI/L 1400 mg NaCI!L I tage 97 of98 i

  • I I i i I I I I I I i I i I I ' ' * * $ I/IJ *<W =-----:-!> £nwiwnnlentaiTCS'IngSoluttonl.kiL Species: Ceriodaphnia dubia 600 mg NaCI/L 800 mg NaCI/L 1000 mg NaCI/L 1200 mg NaCI/L 1400 mg NaCI/L STOCK fage 98 of98 I Page 6 of6 CdNaCLCR #: o S PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different)

___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) Form Approved. OMB No 2040-0004 Add res§_ _E.Q;_BOX 2000 ___________ _ TN0026450 I ! 103 G F-FINAL -__ _.§.ODD_r:_-_Qt.ISY _ _IN 3738L_ ______ _ LOW VOL. WASTE TREATMENT POND Facility_ JYA -_§f:QUOY/lli.NUCLEAR PLANT _____ _ EFFLUENT Locatio.!!_ J::!AMIL TOl:!_COUNTY __________ _ ATIN: Stephapie A Howard From 1 I T .. f I *** NO DISCHARGE CJ ... PARAMETER H 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 00556 0 0 DUITORTHRU PLANT 1 0 0 FFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary MAXIMUM UNITS MINIMUM ******** 7.3 I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance w1th a system designed to assure that qualified personnel properly gather and evaluate the mformation submitted. Based on my inquiry of the person or persons who manage the system, Or those persons dtrectly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false f----------------------1information, includmg the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used NOTE: Read instructions before completinq lh1s form. QUALITY OR CONCENTRATION I NO. !FREQUENCY I SAMPLE EX I OF I TYPE AVERAGE MAXIMUM ******** 8.5 Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE' OFFICER OR AUTHORIZED AGENT UNITS 423 AREA CODE 12 0 GRAB GRAB GRAB GRAB GRAB Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res.§_ BOX 20Q.Q.. ___________ _ -__ _§_ODDY-DAISY _ _JN 3738L ______ _ Facility TVA-SEQUOYAH NUCLEAR PLANT Location HAMIL TON COUNTY = = = = = = = = = = NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 107 G YEAR DAY 28 MAJOR (SUBR 01) F-FINAL METAL CLEANING WASTE POND EFFLUENT NO DISCHARGE *** Form Approved. OMS No. 2040-0004 ATTN: Stephanie A. Howard From I 09 I --I --I NOTE Read instructions before completinq th1s form PARAMETER PH 0 0 LIDS, TOTAL SUSPENDED 0 0 AND GREASE 0 0 TOTAL (AS P) 1 0 0 EFFLUENT GROSS VALUE COPPER, TOTAL (AS CU) 01042 1 0 0 FFLUENT GROSS VALUE RON, TOTAL (AS FE) 0 0 , IN CONDUIT OR THRU ITDCATA1ENT PLANT 1 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P Cleary Sequoyah Site Vice President TYPED OR PRINTED QUALITY OR CONCENTRATION i NO. II FREQUENCY! SAMPLE EX OF i TYPE ANALYSIS ! GRAB MG/L DAILY I COMPOS 19 DAILY GRAB MG/L DAILY 19 I Certify under penalty of law lhat.this document and all attachments w.ere prepared under my 1'\. I TELEPHONE or supervis1on in accordance with a system des1gned to assure that qualified personnel properly gather and evaluate the mformation submitted. Based on my 1nquiry of the person or

  • I persons who manage the system, or those persons responsible for gathering the Pnncipal Envtronmental Engmeer 1nformat1on, the 1nformallon submitted IS , to the best of my knowledge and bel1ef, true, 423 09 DATE ' I j I I 03 ! 10 : accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing VIOlations.

OFFICER OR AUTHORIZED AGENT 1 YEAR I +----i MO I DAYl __L__j COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) F-FINAL Form Approved OMB No. 2040-0004 Add res.§_ _E.!1_;BOX..1.QOO ___________ _ ______ _ Facility_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!L .J.:!AMIL TO!::LCOUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER TEMPERATURE, WATER DEG. CENTIGRADE 00010 z 0 0 INSTREAM MONITORING PH 00400 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 00556 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT TN0026450 YEAR From I 09 I --I -* I QUANTITY OR LOADING AVERAGE MAXIMUM UNITS ******** ******** 04 50050 1 0 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL ******** 50060 0 0 EFFLUENT GROSS VALUE MINIMUM ******** NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Sequoyah Site Vice President I Certify under penally of law that this document and all attachments were prepared under my direction or superv1sion in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the information, the informat1on submitted IS , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false f-----------------------iinformalion, 1ncluding the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320"1 {REV 3/99) Previous editions may be used 110 G DAY 28 RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I ... NOTE: Read instructions before completinq this form QUALITY OR CONCENTRATION I NO. !FREQUENCY SAMPLEl EX I OF TYPE AVERAGE MAXIMUM UNITS 1 ANALYSIS : 0:10. * '()A'Ik¥.JViX Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT .... 19 MG/L TELEPHONE DAILY GRAB-4 WEEKLY I GRAB DAILY I COMPOS 1 I DAlEY GRAB DAILY I GALCTD WEEKLY I GRAB-4 DATE i i 423 843-6700 i 09 i 03 10 AREA I NUMBER MO DAY CODE I I I Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved OMB No. 2040-0004 AddresL ___________ _ TN0026450 ___ ______ _ Facilitv_ JYA -_§!:QUOYAH NUCLEAR PLANT _____ _ Locatio.!!_ _i:jAMIL TOJi.COUNTY __________ _ YEAR ATTN: Stephanie A. Howard Fr om 09 PARAMETER QUANTITY OR LOADING 110 T DAY 28 RECYCLED COOLING WATER EFFLUENT NO DISCHARGE IXXl *** NOTE: Read instructions before this form QUALITY OR CONCENTRATION NO. !FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 1 ANALYSIS IC25 STATRE 7DAY CHR ******** ********

                • ******** t CERIODAPHNIA 23 1 TRP3B 1 0 0 EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Sequoyah Site Vice President TYPED OR PRINTED I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.

Based on my inqUiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are sigmficant penalties for submitting false Information. including the possibility of fine and imprisonment for knowing violations COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 23 TELEPHONE 423 843-6700 I AREA I NUMBER CODE SEMI ANNUAL SEMI COMPOS ANNUAL j R l I I I DATE 'I ! I 1 og 03 10 1 l J I YEAR I MO DAY I _j____J Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different} ___ _ Addres_L 2000 ___________ _ ______ _ Facility_ JYA -_§!:QUOYAH NUCLEAR PLANT _____ _ Locatio.!}_ _!:!AMIL TOJ:LCOUNTY._ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ MAJOR (SUBR 01) TN0026450 116 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER I BACKWASH MONITORING PERIOD EFFLUENT From YEAR DAY 09 To 28 NO DISCHARGE li *** Form Approved OMB No. 2040-0004 ATIN: Stephanie A. Howard NOTE: Read instructions before completinq this form. PARAMETER UI::C"'"* FLOATING (SEVERITY) 1 0 0 NT GROSS VALUE 0 0 EFFLUENT GROSS VALUE QUANTITY OR LOADING AVERAGE I MAXIMUM ******** I ******** I UNITS I MINIMUM I I ******** NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance w1th a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the . . . !information, the information submitted is , to the best of my knowledge and belief, true, Sequoyah Srte Vice President accurate, and complete 1 am aware that there are significant penalt1es for submitting false 1--------------------------l*information, includ1ng the possibility of fine and imprisonment for knowing violatiOns TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes'. EPA Form 3320-1 (REV 3/99) Previous editions may be used QUALITY OR CONCENTRATION I NO. !FREQUENCY I SAMPLE EX OF I TYPE I I I ******** I 0 Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT ANALYSIS J I I 9A 0 ! 1 I 28 VISUAL PASS=O SEE VISUAL FAIL=1 PERMIT .. I 0 II 1 I 28 VISUAL I SEE VISUAL PERMIT TELEPHONE DATE I 1------- 03 10 Page 1 of 1 PERMITTEE NAME/ADDRESS (Include FacilitY Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 Addres.2._ ___________ _ 117 G TN0026450 ______ _ Facility_ .JYA NUCLEAR PLANT _____ _ Locatio.!l_ _jjAMIL TOJ'i.COUNTY __________ _ NO DISCHARGE [J YEAR DAY NOTE: Read instructions before completinq th1s form. From I 09 I --I --I 28 ATTN: Stephanie A. Howard PARAMETER QUALITY OR CONCENTRATION NO. I FREQUENCY/ SAMPLE EX OF i TYPE QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM I AVERAGE I MAXIMUM I UNITS 9A DEBRIS, FLOATING (SEVERITY)

                • ******** 0 ******** ******** 01345 1 0 0 VISUAL EFFLUENT GROSS VALUE OIL AND GREASE. VISUAL VISUAL 84066 0 0 VISUAL EFFLUENT GROSS VALUE I ' ' I ! I I I I i ----,----1 I NAME!TITLE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE I DATE J direction or supervision in accordance with a system designed to assure that qualified personnel I I i Timothy P. Cleary properly gather and evaluate the information submitted.

Based on my inquiry of the person or i persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer I i o3 I 10 \ Information, the information submitted is . to the best of my knowledge and beli.ef, true, 423 843-6700 l 09 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I l l J information, including the possibility of fine and imprisonment for knowing Violations. OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR I MO I DAY l TYPED OR PRINTED CODE I ! COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) F-FINAL Form Approved OMS No. 2040-0004 Addres_L 2000 ___________ _ -__ ...§.ODDY-DAISY _ _IN 37381._ ______ _ Facility TVA-SEQUOYAH NUCLEAR PLANT HAMILTONCOUNn========== ATTN: Stephanie A. Howard PARAMETER OXYGEN, DISSOLVED (DO) 00300 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS VALUE SOLIDS, SETTLEABLE 00545 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 0 EFFLUENT GROSS VALUE TN0026450 YEAR From I 09 I --I --I QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM ******** ******** NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED ---------------- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used 118 G AVERAGE ******** ******** WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE I XX I *** MAXIMUM ******** 19 i, ******** MG/L 19 MG/L 25 MLIL ' ' TWICE/ WEEK i T\tiJICE/ I> GRAB WEEK ONCE/ GRAB MONTH ESTIMA i I I I I I L Oc TELEPHONE DATE Principal Environmental Engineer 423 843-6700 09 03 10 I SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY CODE Page 1 of 1 ENVIRONMENTAL SUBMITTAL FEBRUARY DMR PLEASE SIGN AND RETURN TO Ann Hurt EXT. 6714 DUE IN MAIL TO TDEC BY March 12, 2009 S58 090413 800-NPDES CORRESPONDENCE April 13, 2009 State ofT ennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT -DISCHARGE MONITORING REPORT FOR MARCH 2009 Enclosed is the March 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Timothy P. Cleary V Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, BR 4T-C A. A. Ray, WT 7C-K B. A. Wetzel, OPS 4A-SQN G. R. Signer, WT 6A-K EDMS, WT CA-K (Enclosure) DMR0903.doc REVIEW /CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-MARCH DMR ORGANIZATION: Chemistry and Environmental DOCUMENT PREPARED BY: Ann Hurt DATE: 04/09/2009 EDMS TRACKING NO.: S58 0904 800-NPDES Correspondence CONCURRENCES Name R c Signature -Comment Date v N Ann Hurt X Stephanie A Howard X Alan K Barringer X Debra J Bodine X Beth A. Wetzel X Chris R Church X Timothy P Cleary X (for signature) I INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant April 13, 2009 State ofT ennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

Tennessee Valley Authority Post Office Box 2000 Soddy Daisy, Tennessee 37384-2000 SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR MARCH 2009 Enclosed is the March 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely, Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 pnnted on recycled paper PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ AddresL ..£ 9c_BOX.1.QOO ___________ _ -__ _§_ODD.Y._- DAISY _ _.IN 3738i_ ______ _ Facilitv _ NUCLEAR PLANT _____ _ Locatio..!}_ _!:!AMIL TOl!_COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT IDMRJ r--rNoo2645o 101 G MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT Form Approved. OMB No. 2040-0004 NO DISCHARGE CJ *** ATIN: Stephanie A. Howard From __ __ _. 31 DAY NOTE: Read instructions before completinQ this form. PARAMETER e><: QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY I SAMPLE 1 EX OF ' TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. I SAMPLE ******** ********

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  • * **:* *so* EFFLUENT GROSS VALUE DEG.C. [. 90NTIN lcALCTO PH SAMPLE ******** ******** ** 8.0 ******** 8 4 MEASUREMENT . 12 100400 1 0 0 iEFFLUENT GROSS VALUE !SOLIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 00556 0 0 EFFLUENT GROSS VALUE PERMIT REQUIREMENT SAMPLE 1 MEASUREMENT 1 1*** PERMIT*.*
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__ * ****_._* __ *2l***_.-... _-_-_ ** IEFFLUENT GROSS VALUE REQUIREMENT ' DAILy MX ' .. -' . *.* . .. ' '. * .. l_!"_AMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s and all attachments were prepared under my r TELEPHONE I DATE direction or supervision in accordance wtth a system des1gned to assure that qual1f1ed personnel 09 I 04 I Timothy P Cleary properly gather and evaluate the 1nformat1on submitted Based on my of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer I informat1on, the informat1on subm1tted 1s , to the best of my knowledge and belief, true, 423 843-6700 09 Sequoyah Site Vice President accurate, and complete I am aware that there are Significant penalties for subm1tt1ng false SIGNATURE OF PRINCIPAL EXECUTIVE I Y-EARr-M'O -c information, including the possibility of ftne and tmpnsonment for knowtng violations OFFICER OR AUTHORIZED AGENT AREA I NUMBER DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment:

1. CCW data 2. veliger monitoring data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2 DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 03/11/2009@

1232 0.15mg/l 03/12/2009@ 1721 KLM TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 03/11/2009@ 1234 <0.11 mg/1 03/12/2009 @ 1732 KLM TN EPA 8015 Mean# of Water Temp. Mean# of Water Temp. LOCATION SUB NOTES:% Sample Date ZM/m3 % Settlers ('C) Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY Clams/m3 ('C) Clam 11/21/2008 0 0 12 11/16/2008 230 12 In plant RCW Dick Adcock 11/24/2008 31 0 11 11/24/2008 138 11 In plant RCW Dick Adcock 12/4/2008 0 0 10 12/04/2008 15 10 In plant RCW Dick Adcock 12/812008 0 0 9 12/08/2008 0 9 In plant RCW Dick Adcock 12/1512008 0 o* 9 12/15/2008 0 9 In plant RCW Dick Adcock 12/2212008 0 0 9 12/22/2008 0 9 In plant RCW Dick Adcock 12129/2008 0 0 9 1212912008 0 9 In plant RCW Dick Adcock 01/09/2009 1"1 0 Q 01/09/2009 0 8 In plant RCW Dick Adcock v u 0111612009 0 0 7 0111612009 0 7 In plant RCW Adcock I Bryant 01122/2009 0 0 7 0112212009 0 7 In plant RCW Adcock I Bryant 01/30/2009 0 0 9 01/3012009 0 9 In plant RCW Adcock I Bryant 0210612009 0 0 6 0210612009 0 6 In plant RCW Adcock I Bryant 0211312009 0 0 11 0211312009 0 11 In plant RCW Love 0212012009 0 0 9 0212012009 0 9 lnplant RCW Bryant 0212612009 0 0 9 0212612009 0 9 In plant RCW Bryant 0310612009 0 0 8 0310612009 0 8 In plant RCW Dick Adcock 0311312009 0 0 10 0311312009 0 10 In plant RCW Dick Adcock 0312712009 0 0 14 0312712009 0 14 In plant RCW Wayne Wright PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res.§_ _E ___________ _ -__ _§ODD_1_-DAISY __ :fl::!.]738i_ ______ _ Facility _ _IYA -.§E:QUOYAH NUCLEAR PLANT _____ _ Locatio.!]_ ___I::!AMIL TO.!i_COUNTL _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER M.illllTORING PERIOD YEAR DAY From 09 To 31 MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT *** NO DISCHARGE D ... Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard NOTE: Read instructions before completinq this form. PARAMETER I No. [FREouENCY SAMPLEl EX I OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 0.036 0 18 I 31 GRAB CHLORINE, TOTAL RESIDUAL ******** ******** ******** 0.018 19 ! 150060 1 0 0 PERMIT ******** I REQUIREMENT

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                • 2 62 1 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ,1 Cert1fy under penalty of law that th1s document and all attachments were prepared under my d"ection or superviSion in accordance with a system des*gned to assure that qualified personnel Timothy P. Cleary *properly gather and evaluate the information submitted.

Based on my inqu1ry of the person or 1 I persons who manage the system, or those persons d"ectly responsible for gathemg the . . . information, the information submitted is , to the best of my knowledge and bel1ef, true, Sequoyah S1te V1ce PreSident accurate, and complete. 1 am aware that there are s*gn1f1cant penalties for submitting false 1nformat1on, including the possibility of tine and Imprisonment for know1ng violations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured: 1. Biodetergent 73551 (max. calc. cone. was 0.027mg/L--Iimit 2.0mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used ******** TELEPHONE Principal Environmental Engineer 423 843-6700 09 04 09 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO I DAY CODE Page 2 of 2 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L _E.fLBOX..1.QQQ_ ___________ _ ___ ______ _ Facility_ JYA -_g:QUOYAH NUCLEAR PLANT _____ _ Locatioll_ _!:jAMIL TOJ:i.COUNTL _________ _ ATIN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMRJ TN0026450 101 Q PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD From I I I To I MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE D *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. !FREQUENCY EX OF SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM BORON, TOTAL ******** ******** I 1 o1 022 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIBE'MENT. SAMPLE MEASUREMENT SAMPLE MEASUREMENT "' PERMI]:':f'i? SAMPLE MEASUREMENT PERMIT REQUIR8.M,ENT FMEITITLE PRINCIPAL EXECUTIVE OFFICER I Cerlify under penalty of law that th1s document and all attachments were prepared under my d1rection or supervision 1n accordance w1th a system designed to assure that qualified personnel ' Timothy P. Cleary ' properly gather and evaluate the mformation subm1tted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the . . . information, the information submitted IS , to the best of my knowledge and belief, true, Sequoyah Site V1ce Pres accurate, and complete. 1 am aware that there are significant penalties for submitting false tnformatlon, tnclud1ng the posstbtllty of fine and 1mpnsonment for know1ng vtolations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Boron was sampled on 01/07/2009@ 1005. EPA Form 3320-1 (REV 3/99) Previous editions may be used <0.85 0, cikwcv<.d Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT UNITS ANALYSIS 19 0 2/90 TELEPHONE DATE 423 843-6700 09 04 09 I AREA l NUMBER YEAR MO DAY CODE __ L_ Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres.L_ .J: ___________ _ -__ __§_ODDY-DAISY _ _JN ______ _ Facility_ JYA NUCLEAR PLANT:_ ____ _ Locatio.!l._ .J:!AMIL TO.!!_ COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 101 T DAY From 31 MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 BIOMONITORING FOR OUTFALL 101 EFFLUENT *** NO DISCHARGE D ... NOTE: Read instructions before completinq this form. I NO. . EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS I ANALYSIS t:-: -"'I I IC25 STATRE 7DAY CHR SAMPI F ******** I ******** CERIODAPHNIA TRP3B 0 0 EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 0 EFFLUENT GROSS VALUE SAMPLE ! MEASUREMENT PERMIT SAMPLE MEASUREMENT " PER!vl.ti';,* .* * ... .;;;J'< ' -,; PERMIT REQUIREMENT:

  • NAMErTITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my direction or supervision m accordance w1th a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Information, the information submitted is , to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submittmg false information, 1ncluding the poss1b1lity of fine and 1mpnsonment for know1ng VIOlations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in March 2009. EPA Form 3320-1 (REV 3/99) Previous editions may be used ******** ******** 23 d TELEPHONE DATE Principal Environmental Engineer 423 843-6700 09 04 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREA l NUMBER YEAR MO CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ TN0026450 103 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 ___ DAISY _ __I!::!-'!7381._ ______ _ LOW VOL. WASTE TREATMENT POND Facility_ JYA -_§!:QUOYAH NUCLEAR PLANT _____ _ Locatio.!l._ _HAMIL TO.!:!_ COUNTy_ _________ _ EFFLUENT ATTN: Stephanie A. Howard DAY 31 *** NO DISCHARGE D ... PARAMETER 1 PH 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 0 0 EFFLUENT GROSS VALUE OIL AND GREASE I loo556 1 o o !EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 0 EFFLUENT GROSS VALUE NOTE: Read instructions before completinq this form. '"j<i QUANTITY OR LOADING QUALITY OR CONCENTRATiON___ FREQUENCY I SAMPLE OF TYPE AVERAGE MAXIMUM MINIMUM AVERAGE MAXIMUM ANALYSIS - ',

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... NT 'c> c;>,:;--,,,' --...,___::___- I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my 'Ct. TELEPHONE I DATE direction or supervision in accordance with a system des1gned to assure that qualified personnel I I 09 Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inqUiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted 1s , to the best of my knowledge and belief, true, 423 843-6700 I 09 04 I Sequoyah Site Vice President I accurate, and complete I am aware that there are S1gnif1cant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of f1ne and impnsonment for know1ng VIOlations OFFICER OR AUTHORIZED AGENT AREA I NUMBER I YEAR MO l DAY ' TYPED OR PRINTED CODE ! COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 I PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Addres,L _f ___________ _ -__ __§_ODD_Y._- DAISY _ _IN 37381_ ______ _ Facility_ .J.YA -_lli:QUOYAH NUCLEAR PLANT _____ _ LocatiOIL. _jjAMIL TOl!_COUNTY._ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 107 G PERMIT NUMBER DISCHARGE NUMBER YEAR DAY From 09 31 MAJOR (SUBR 01) F-FINAL METAL CLEANING WASTE POND EFFLUENT *** NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER PH SAMPLE MEASUREMENT QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM ******** ******** QUALITY OR CONCENTRATION I NO. I FREQUENCY EX OF SAMPLE I TYPE AVERAGE MAXIMUM UNITS ANALYSIS --+-******** 00400 0 0 EFFLUENT GROSS VALUE ,SOLIDS, TOTAL SUSPENDED 1 00530 1 0 0 GROSS VALUE OIL AND GREASE 00556 1 0 0 EFFLUENT GROSS VALUE PHOSPHORUS, TOTAL (AS P) 00665 0 0 EFFLUENT GROSS VALUE COPPER, TOTAL (AS CU) I I lo1o42 1 o o :** PER'MIT o\;r>:i{:.,

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' . . . -"** 'OAitYMX GROSS VALUE ... -** ***-...... [IRON, TOTAL (AS FE) [01045 1 0 0 1 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 1 5oo5o 1 o o [EFFLUENT GROSS VALUE * *MQ A.V,G.*.* .. . DAILYMX . L__ __ 12 ! 19 MG/L 19 TELEPHONE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this document an.d all attachments were P. repared under my Lj.-, j ol J! . d. direCtiOn or superv1s1on in accordance w1th a system des1gned to assure that qual1f1ed personnel q -* DATE J 09 I Timothy P. Cleary properly gather and evaluate the 1nformat1on subm1tted. Based on my 1nqUify of the person or personswho manage the system. or those persons directly responsible for gathenng the Principal Environmental Engineer 1 423 . . . 1nformat1on, the information submitted IS , to the best of my knowledge and bel1ef, true, 1 843-6700 Sequoyah S1te V1ce President accurate. and complete 1 am aware that there are significant penalt1es for submtttlng false SIGNATURE OF PRINCIPAL EXECUTiVE! _ 'I '--------*-------------1'nformatlon. 1nclud1ng the poss1b1l1ty of fine and 1mpnsonment for know1ng VIOlations OFFICER OR AUTHORIZED AGENT-NUMBER TYPED OR PRINTED I CODE YtoAKI -------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 ! 04 09 i MO i DAY I PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res.§_ ___________ _ -__ _§ODDY-DAISY _ _IN 3738+/-_ ______ _ Facility_ -.§l:QUOYAH NUCLEAR PLANT _____ _ Locatio.o_ __!:!AMIL TO.!'!_ COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER PH ,00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED !00530 1 0 0 EFFLUENT GROSS VALUE ! OIL AND GREASE 00556 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 0 'EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT (OMRJ MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 TN0026450 PERMIT NUMBER YEAR From 09 QUANTITY OR LOADING *:REPCDRT* DAILYMX ******** MGD 110 G DISCHARGE NUMBER DAY 31 RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** NOTE: Read instructions before completinQ this form. -*--. NO. [FREQUENCY QUALITY OR CONCENTRA"IIUN EX I OF AI\JAIYSIS l .... _ ..... -.... ! I I .. *-1 ******** ******** 50060 0 0 PERMIT******* REQUIREMENT DAILYMX. MG/L GRAB-4 EFFLUENT GROSS VALUE L *.x. I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my direction or superviSIOn in accordance w1th a system des1gned to assure that qualified personnel i Timothy P Cleary properly gather and evaluate the information submitted Based on my 1nqu1ry of the person or I persons who manage the system, or those persons d1rectly respons1ble for gathenng the 1nformat1on, the 1nformatton submitted is , to the best of my knowledge and belief, true. Sequoyah Site Vice President accurate, and complete I am aware that there are Slgnif!cant penalties for submitting false I 1nformat1on, 1ncludmg the possibil1ty of fine and Imprisonment for knowtng VIOlations. c TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used TELEPHONE DATE I Principal Environmental Engineer 423 843-6700 09 04 l 09 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO l DAY CODE Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L _E ___________ _ -__ _§ODD _::f._-DAISY _ _IN 37384.._ ______ _ Facility_ JYA NUCLEAR PLANT _____ _ _!:!AMIL TOJ:i_COUNTJ'_ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 PERMIT NUMBER YEAR From! 09 I --MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREOUENCYI SAMPLE EX OF TYPE PARAMETER AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR ******** ******** ******** ******** CERIODAPHNIA SEMI !cOMPOS c I TRP3B 1 0 0 EFFLUENT GROSS VALUE PIMEPHALES I MEASUREMENT TRP6C 1 0 0 EFFLUENT GROSS VALUE

L c'PERMIT ..... . ___ I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this document and all attachments were prepared under my direction or supervtsion tn accordance w1th a system designed to assure that qualified personnel ! I I i Timothy P Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the informat1on, the information submitted is, to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete I am aware that there are significant penalties for submitting false Information, 1nclud1ng the poss1bil1ty of fme and Imprisonment for knowing violations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Q dkru_xu_d Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT I TELEPHONE DATE 091 I 423 843-6700 04 09 J 'AREA I NUMBER YEARJ MO DAY CODE Page 1 of 1 PERMIITEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 AddresL ___________ _ -__ _§_ODD.1_- DAISY _ _]N 3738L ______ _ Facility_ JYA -..§EQUOYAH NUCLEAR PLANT _____ _ Locatio.!l._ _!:!AMIL TOJ:i.COUNTY __________ _ ATTN: Stephanie A Howard PARAMETER DEBRIS, FLOATING (SEVERITY) 01345 1 0 0 [EFFLUENT GROSS VALUE lOlL AND GREASE VISUAL ;84066 1 0 0 I EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT I TN0026450 r-----------YEAR From I 09 I n I -* I QUANTITY OR LOADING AVERAGE MAXIMUM MINIMUM ******** ******** ******** 116 G 31 NO DISCHARGE CJ *** DAY NOTE: Read instructions before completinq this form. QUALITY OR CONCENTRATION I NO. [FREQUENCY[ SAMPLE EX OF [ TYPE ANALYSIS AVERAGE MAXIMUM UNITS 1 I 31 VISUAL ******** 0 0 9A PASS=O FAIL=1 ol IVI""L. I ******** VISUAL -*VISUAL i NAME/TITLE PRINCIPAL EXECUTIVE OFFICER it Cert1fy under penalty of law that th1s document and all attachments were prepared under my . 0, .ilo1.uc(.."- d TELEPHONE DATE I !?lrect1on or superviSIOn 1n accordance w1th a system des1gned to assure that qual1f1ed personnel I Timothy P CI"'Y . '"'"'" '"' """"" "" oo m, '"'"'" o1 """" m persons who manage the system or those persons d1rectly responsible for gathenng the Principal Environmental Engineer 423 843-6700 09 04 09 . Information the 1nformat1on submitted 1s to the best of my knowledge and bel1ef, true, Sequoyah Stte Vtce Prestdent accurate and complete 1 am aware that there are s1gmf1cant penalt1es for submtt!ing false SIGNATURE OF PRINCIPAL EXECUTIVE I 1nformat1on 1ncJudJng the possibility of fme and 1mpnsonment for know1ng VIOlations OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR I MO DAY TYPED OR PRINTED CODE -----COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res§_ ___________ _ -__ _§_ODD_1_- DAISY _ _It:!.]7381_ ______ _ Facility _ _I! A -.§!:QUOYAH NUCLEAR PLANT _____ _ Locatio.!]_ ..J:::!AMIL COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ C=rNoo264so [ 117 G I PERMIT NUMBER DISCHARGE NUMBER MONITORINr, PERIOD YEAR I MO I DAy I I YEAR I MO I DAy From 09 I 03 I 01 I Tor 09 1 03 I 31 MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT NO DISCHARGE CJ *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. IFREQUENCYI SAMPLE DEBRIS, FLOATING (SEVERITY) ' 1 01345 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE VISUAL AVERAGE ******** ********', ******** MAXIMUM MINIMUM ******** ******** ********' 0 EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS 0 9A 0 1 /31 VISUAL ******** I I .* X * ** I *ow I I " F I *. " I'

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'PERMIT . REQUIREMENT -,:>"'> ********* 1.1-:-J .* (\ II ' . *' TELEPHONE DATE I yt\.L(J"'\0.-tt..U. LA .. d . Principal Environmental Engineer 423 843-6700 09 04 ::9Y I SIGNATURE OF PRINCIPAL EXECUTIVE I ! OFFICER OR AUTHORIZED AGENT AREAl NUMBER I YEAR MO DAY , CODE , I NAMEfTITLE PRINCIPAL EXECUTIVE-OFFICER-TI Cert1fy under penalty of law that this document and all attachments were prepared under my -jdlrect1on or superviSion 1n accordance w1th a system destgned to assure that qualified personnel I Timothy P. Cleary [properly gather and evaluate the information submitted. Based on my inqwy of the person or persons who manage the system, or those persons directly respons1ble for gathenng the I . . , information, the information submitted !S , to the best my knowledge and belief, .true, 1 Sequoyah S1te V1ce President accurate, and complete 1 am aware that there are s1gnlf1cant penalties for submitt1ng false I I information, 1nclud1ng the possibility of fine and imprisonment for knowing violations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location 1f Different) ___ _ Add res.§_ ___________ _ ______ _ Facility_ JYA -.§!:QUOYAH NUCLEAR PLANT _____ _ Location_ J::!AMIL TO_!::!_ COUNTY __________ _ ATIN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 118 G PERMIT NUMBER DISCHARGE NUMBER MQNITORING PEflli2D YEAR I YEAR I MO DAY From I 09 To 09 03 31 MAJOR (SUBR 01) F-FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved OMB No. 2040-0004 NOTE: Read instructions before completinQ this form. I PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.-rREQUENCY 1 SAMPLE 1 i IOXYGEN, DISSOLVED (DO) I 100300 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS VALUE SOLIDS, SETTLEABLE 00545 1 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU \TREATMENT PLANT isoo5o 1 o o GROSS VALUE AVERAGE SAMPLE ******** MEASUREMENT PERMIT REQUIREMENT SAMPLE ******** MEASUREMENT 1.*. PE;gMIT

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                • [_NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my ,J&pllCUU2 6

J direction or supervision 1n accordance with a system designed to assure that qua!1fied personnel I Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inqUiry of the person or I persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer Information, the 1nformat1on subm1tted IS , to the best of my knowledge and belief, true, accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I 19 MG/L I 19 MG/L 25 ML/L EX OF TYPE , ANALYSIS ' I TWICE/ I G.RAB WEEK.. *, J'WICE/ .WEEK . ONCE/ MONTH BATGH .. GRAB ESTIMA: ! I TELEPHONE I DATE I I 423 843-6700 I 09 04 09 I I i Site v;oo p,,;dool tnformat1on, 1nc!uding the poss1b1hty of fine and imprisonment for know1ng VIOlations. OFFICER OR AUTHORIZED AGENT AREA I NUMBERtvEAR MO DAY l PED OR PRINTED CODE l COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 S58 090514 800-NPDES CORRESPONDENCE May 14, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT -DISCHARGE MONITORING REPORT FOR APRIL 2009 Enclosed is the April 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at {423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely,.

. II (1 JJ"Q *4 . '

... ,. !jJ_,_L_, \1 f Timothy P.JCieary /j Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, BR 4T-C A. A. Ray, WT 7C-K B. A. Wetzel, OPS 4A-SQN G. R. Signer, WT 6A-K EDMS, WT CA-K (Enclosure) DMR0904.doc REVIEW/CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-APRIL DMR ORGANIZATION: Chemistry and Environmental DOCUMENT PREPARED BY: Ann Hurt DATE: 05/11/2009 CATEGORY: NPDES Correspondence Name Ann Hurt R v X c N CONCURRENCES Signature -Comment Date Stephanie A Howard X Alan K Barringer Debra J Bodine Beth A. Wetzel Chris R Church Timothy P Cleary (for signature) X X X X X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 May 14, 2009 State ofT ennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT -DISCHARGE MONITORING REPORT FOR APRIL 2009 Enclosed is the April 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely, _./ 11 (); ( rJ!.e-'\'-"-l Cleary I Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res.§_ ___________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDE$) DISCHARGE MONITORING REPORT (OMR) TN0026450 101 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 ______ _ PERMIT NUMBER DISCHARGE NUMBER] DIFFUSER DISCHARGE MONITORING E'.ERIOD EFFLUENT Facility _ _II/A NUCLEAR PLANT _____ _ Locatio!}_ .J::!AMIL TOJ::LCOUNT:L_ _________ _ From YEAR DAY 09 To 30 CJ *** *** NO DISCHARGE ATTN: Stephanie A. Howard NOTE: Read instructions before completinQ this form. PARAMETER c:><: QUANTITY OR LOADING I QUALITY OR CONCENTRATION NO. FREQUENCY! EX OF TYPE i AVERAGE j MAXIMUM UNITS MINIMUM -1 AVERAGE I MAXIMUM I UNITS ANALYSIS !TEMPERATURE, WATER DEG. SAMPLE ******** I ******** ** I ******** I 20.8 ' 0 30 I 30 MODELD jCENTIGRADE MEASUREMENT i I 04 iooo1 o z o o r--PERMIT -******** I ******** !INSTREAM MONITORING REQUIREMENT

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  • PERMIT ******** 30.2 0 I 30 I 30 RCORDR 04 CENTIGRADE 00010 1 0 0 EFFLUENT GROSS VALUE I TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 1 00016 1 s 0 t:FFLUENT GROSS VALUE iPH I SAMPLE MEASUREMENT
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.,, .:*,,, '> , GROSS VALUE < ;:;,.::*'* ' MX i IL AND GREASE SAMPLE ******** ******** ** ******** <6 <6 0 1 I 30 GRAB ' MEASUREMENT 19 1 0 0 GROSS VALUE 1FLOW, IN CONDUIT OR THRU !TREATMENT PLANT ','HERMIT < **t** REbUIR_EMENt

  • .* SAMPLE MEASUREMENT

.. 4;; ----F--'-----'-+'-'__c,____.__+---_......, 15oo5o 1 o o GROSS VALUE PERMIT REQUIREMENt .. ******** 1386 ******** REP.ORT DAilYMX 03 MGD ******** .. ******** ******** 0 **** PRINCIPAL EXEMIVE under penalty of law that this document and all attachments were prepare.d under my I rw .. . ' -(\ (I I ' ., . **c . d TELEPHONE DATE --d1rect1on or superv1s1on 1n accordance w1th a system des1gned to assure that qual1f1ed personnel --:)11 f) .Qt lG:JL..-l.JL.lA '-L.I(f vz..._. A..\. . , Timothy p Cleary I properly gather and evaluate the Information submitted. Based on my 1nqU1ry of the person or / * ""-"1' 1 persons who manage the system, or those persons responsible for gathenng the Principal Environmental Engineer 6700 09 5 11 1 1 1nformat1on the 1nformat1on subm1tted IS . to the best of my knowledge and belief, true. 423 843-0 Sequoyah Site Vice President I accurate. and complete 1 am aware that there are s1gmf1cant penalt1esfor subm1ttmg false , SIGNATURE OF PRINCIPAL EXECUTIVE I =rformat1on. 1ncludtng the possib1i1ty of f1ne and 1mpnsonment for know1ng v1olat1ons I OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY I = TYPED OR PRINTED ____ CODE __:"j COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment:

1. CCW data 2. veliger monitoring data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2 DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 04/08/2009@

1423 0.11 mg/1 04/10/2009 @ 0944 JOB TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 04/08/2009 1422 <0.10 mg/1 04/1 0/2009 @ 0954 JOB TN EPA 8015 Mean# of Water Temp. Mean# of Water Temp. LOCATION SUB NOTES:% Sample Date ZM/m3 % Settlers ('C) Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY Clams/m3 ("C) Clam 1112112008 0 0 12 1111612008 230 12 lnplant RCW Dick Adcock 1112412008 31 0 11 1112412008 138 11 lnplant RCW Dick Adcock 121412008 0 0 10 1210412008 15 10 In plant RCW Dick Adcock 121812008 0 0 9 1210812008 0 9 In plant RCW Dick Adcock 1211512008 0 0 9 1211512008 0 9 In plant RCW Dick Adcock 1212212008 0 0 9 1212212008 0 9 In plant RCW Dick Adcock 1212912008 0 0 9 1212912008 0 9 In plant RCW Dick Adcock 0110912009 0 0 8 0110912009 0 8 In plant RCW Dick Adcock 0111612009 0 0 7 0111612009 0 7 In plant RCW Adcock I Bryant 0112212009 0 0 7 0112212009 0 7 lnplant RCW Adcock I Bryant 0113012009 0 0 9 0113012009 0 9 In plant RCW Adcock I Bryant 0210612009 0 0 6 0210612009 0 6 lnplant RCW Adcock I Bryant 0211312009 0 0 11 0211312009 0 11 In plant RCW Love 0212012009 0 0 9 0212012009 0 9 In plant RCW Bryant 0212612009 0 0 9 0212612009 0 9 lnplant RCW Bryant 03106/2009 0 0 8 0310612009 0 8 lnplant RCW Dick Adcock 0311312009 0 0 10 0311312009 0 10 In plant RCW Dick Adcock 0312712009 0 0 14 0312712009 0 14 lnplant RCW Wayne Wright 03130/2009 0 0 13 03/30/2009 0 13 In plant RCW Dick Adcock 04/09/2009 28 0 14 04/09/2009 0 14 In plant RCW Dick Adcock PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L __f_ ___________ _ -__ _J?_ODD_i..- DAISY _ _Ifi]738i_ ______ _ £E.cilitv _ NUCLEAR PLANT _____ _ Locatio_ll_ _!:!AMIL TOJ:i_COUNT'L_ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) TN0026450 I 101 G I DISQHARGE NUMBER From MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE [] *** Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard c__ _ __L ___ NOTE: Read instructions before completinq this form. 1-- 'I NO. I FREQUENCY SAMPLEl I I . EX OF TYPE AVERAGE MAXIMUM UNITS I MINIMUM AVERAGE MAXlMUM UNITS ANALYSIS :cHLORINE, TOTAL RESIDUAL 19 35 I 30 ******** ******** ******** 0.025 0.052 0 GRAB !50060 1 0 0 PERMIT ******** ******** !EFFLUENT GROSS VALUE REQUIREMENT

      • -. .,.. --" .... , .... !TEMPERATURE-C, RATE OF SAMPLE . . . 4 [CHANGE 1 MEASUREMENT 82234 1 0 0 GROSS VALUE r PERMIT REQUIREMENT I SAMPLE MEASUREMENT PEBMIT -.. _ *.

SAMPLE MEASUREMENT SAMPLE 1 **" -------------

:

I L !NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my ;', j *\ ..J 1 d I TELEPHONE DATE r----- direction or superviSIOn In accordance with a system destgned to assu_re that qualified personnel r {.c (\ lGt LU ()i (4 t,./ .... t,.V( t.-Z I I Timothy p_ Cleary properly gather and evaluate the 1nformat10n submitted Based on my 1nqu1ry of the person or /'. '1-' J -persons who manage the system,or those persons directly responsible for gathenng the PrtnCipal Environmental Engineer 3 ?OO 09 05 11 I * .

  • 1 1nformat1on, the 1nformat1on submitted 1S, to the best of my knowledge and bel1ef, tnue, 42 843-6 : I Sequoyah Stte Vice President

!accurate. and complete I am aware that there are sign1f1cant penalt1es for submttt1ng false SIGNATURE OF PRINCIPAL EXECUTIVE ___j____ _ j r--------'Information, 1nciud1ng the poss1b1llty of f1ne and tmpnsonment for know,ng VIOlatiOns OFFICER OR AUTHORIZED AGENT 1 NUMBER YEAR MO Jl DAY ! , Tr:_PED OR PRINTE_r:J_ _ CODU COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 2 of 2 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT !DMR) MAJOR (SUBR 01) Form Approved. OMB No. 2040-0004 Addres.§_ ..£ ___________ _ TN0026450 101 T F-FINAL -__ _20DD_}'_- DAISY _ _Ii':!.]738i_ ______ _ PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facility _ _IYA-PLANT _____ _ EFFLUENT Locatio!!_ _I::!AMIL TO.i:!_COUNTY._ _________ _ MONITORING PERIOD [:] MO I DAY I DAY ATTN: Stephanie A. Howard PARAMETER STATRE ?DAY CHR jcERIODAPHNIA jTRP3B 1 0 0 !EFFLUENT GROSS VALUE IIC25 STATRE ?DAY CHR IPIMEPHALES jTRP6C 1 0 0 !EFFLUENT GROSS VALUE . :"tpERMIT SAMPLE MEASUREMENT

F*'PERMI}'*.***:.*.

PERMIT REQUIREMENT NO DISCHARGE D ... Fromos 04 I 01 To 30 NOTE: Read instructions before completinQ this form. QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. I FREQUE-NCYT SAMPLE EX OF I TYPE AVERAGE MAXIMUM ******** ******** UNITS MINIMUM Monitoring Not Reauired AVERAGE MAXIMUM UNITS ******** ******** 23 r NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my r. . 1 1. . 1 TELEPHoNE 1 oATE

  • ,:-[ I direct1on or supervision in accordance with a system designed to assure that qualified personnel Timothy P Cleary properly gather and evaluate the tnformation submitted.

Based on my tnqu1ry of the person or persons who manage the system, or those persons dtrectly responstble for gathenng the *' , Principal Environmental 4 23 843-6700 I 09 05 11 rnformatron, the rnformatron submrtted rs , to the best of my knowledge and belief, true. Sequoyah Site Vice President accurate, and complete. I am aware that there are signtficant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE --I ----Information, inc!udtng the posstb!ltty of fine and impnsonment for knowing vtolattons OFFICER OR AUTHORIZED AGENT DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) I* MC.CM l NUMBER [YEAR I MO J ___________ .L. :::,CO:=.-D::':E=-LI _ _L __ ____j Toxicity was not sampled in April 2009. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved OMS No. 2040-0004 ___ ___§_ODD ... !: .. : DAISY _ _IN 3738+/-..._ ______ _ LOW VOL. WASTE TREATMENT POND Facility_ -.§E:QUOYAH NUCLEAR PLANT _____ _ Locatio_12_ ...J::!AMIL TOl:!_COUNTL _________ _ EFFLUENT DAY 30 *** NO DISCHARGE [J *** A TIN Stephanie A Howard NOTE Read instructions before completinq this form. I EX OF TYPE I ---,ARAMmR <:]--QUANTITY oR LOADING QUALITY OR coNCENTRATION .. NO. FREOUENcYI s-AMPLE -/ AVERAGE I MAXIMUM I UNITS MINIMUM I AVERAGE I MAXIMUM I UNITS ANALYSIS ******** ******** 7.2 ******** 8.5 PH SAMPLE .. ! MEASUREMENT 12 I 0 15 f 30 GRAB EFFLUENT GROSS VALUE ! . . . r00400 1 0 0 * * . PERI\i11T,

  • RSQUfgEMENJ;1 SU THREE/ , GRAB SOLIDS, TOTAL SUSPENDED
                • 9 SAMPLE MEASUREMENT 134 284 26 15 19 7/30 GRAB I i00530 1 0 0 . PERMIT '380. LBS/DY <

' 100 !EFFLUENT GROSS VALUE REQUIREMENT . MO AVG MO AVG ';} DAILY MX lolL I sAMPLE <78 <1 06 ******** <6 <6 -' MEASUREMENT I 26 MG/L WECKL yl GRAB 19 I I GRAB !00556 1 0 0 .. :: t9()-. GROSS VALUE MO:'J(VG *. .. < jFLOW, IN CONDUIT OR THRU SAMPLE I 1.437 ******** ******** ........... TREATMENT PLANT MEASUREMENT 03 ******** MG/L r

l. 9:8 ** 1 0 I :SU I :SU I TOT ALZ 50050 1 0 0 t' PERM!T . EFFLUENT GROSS VALUE :f3E.QYIREMENT
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"PERMIT X REQUIREfv1EI'JT , MGD I ** .. SEE ***I*.T_ .* _PTALZ PERMIT **: -r E/TITLE PRINCIPAL EXECUTIVE OFFICER I under penalty and a. II attachm.;;;!s w_ere p. ,.;pared 0Jf __ *.* ...,...-DATE Timothy P. Cleary properly gather and evaluate the 1nformat1on submitted Based on my 1nqU1ry of the person or '--'L4J -"" 'v * -* '--i 1 , -. d1rect1on or superv1s1on 1n accordance w1th a system des1gned to assure that qualif1ed personnel \.t (< i(i fl,i..iL; J{ *41(/.l* .{tA.O. i persons who manage the system, or those persons d11ectly responsible for gathenng the Principal Environmental Engineer I I * . . . 1nformat1on, the 1nformat1on subm1tted 1S, to the best of my knowledge and bel1ef. true. 423 843-6700 I 09 05 1 11 . .. Sequoyah S1te Vtce President I accurate, and complete 1 am aware that there are s1gn1ficant penalt1es for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I -+--__ 1 ! _1 1nclud1ng the posslbil.ty of f1ne and 1mpnsonment for know1ng Violations OFFICER OR AUTHORIZED AGENT r-ARE!\ 1 NUMBER I YEAR MO I DAY TYPED OR . COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The Turbine Building Sump (TBS) was discharged directly to the Yard Pond (YP) from 4/16@ 0319 to 4/17@ 1915. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ ___ DAISY _ _IN 37381_ ______ _ £9_ctlitv _ _IYA NUCLEAR PLANT _____ _ Locatio.!l_ .J:!AMIL TOJ:iCOUNT:L_ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) [ TN0026450 I I 107 G I I PERMIT NUMBER :J I DISCHARGE NUMBER i MONITORING PERIOD YEAR From 09 i To MAJOR (SUBR 01) F-FINAL METAL CLEANING WASTE POND EFFLUENT *** NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard '-----'------'----- NOTE: Read instructions before completinq this form. I PARAMETER QUANTITY oR LOADING ---QUALITY oR coNCENTRAnoN NO.I"""'"" SAMPLE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANA-L-YS_I_S-+------1 EX OF TYPE PH ******** ******** ** ******** 12 I 100400 1 0 0 **** s;;*c.,9.0 . SU GRAB !EFFLUENT GROSS VALUE MAXIMtillll I SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ** ******** ******** i MEASUREMENT 19 1 00530 1 0 0 **** lc\,; . j - .... *.* .. *.Ii.*.**. <;.* .. *..* !EFFLUENT GROSS VALUE * < _> ,*DAILY MX .. IOIL AND GREASE ]00556 1 0 0 IEFFLUENT GROSS VALUE PHOSPHORUS, TOTAL (AS P) 00665 0 0 SAMPLE i MEASUREMENT .PERMIT.}t REO\.IIRErvJEt.J::r;I'H , r 'Ad'< -SAMPLE MEASUREMENT

                • ********

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  • 101 045 1 0 0 PERMIT,.. . rFLUENT GROSS VALUE
        • =Low, IN coNDUIT OR THRU 1 sAMPLE . rREATMENT PLANT MEASUREMENT 03 ******** ********

,, M:iC ******** 150050 1 0 0 GROSS VALUE ?P.ERMIT t, . /DAlLYMX' I **** I > I MGD i NAMEffiTLE PRINCIPAL EXECUTIVE OFFICER t Certify under penalty of law that thts document and all attachments were prepared under my ---------. dtrection or superviston tn accordance with a system designed to assure that qualified personnel Timothy P Cleary properly gather and evaluate the tnformatton submttted. Based on my tnqutry of the person or I persons who manage the system, or those persons dtrect!y responsible for gathertng the i tnformatton, the tnformation submttted ts, to the best of my knowledge and beltef, true, ! Sequoyah Site Vice President accurate, and complete ! am aware that there are s1gniflcant penalt1es for subm1tt1ng false ' JnformatJon, Including the possibility of fme and 1mpnsonrnent for know1ng vtoiations ---* TYPED OR PRINTED ! _____________ ......_._L_____ _____

  • COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used --\ . "d TELEPHONE DATE I

!j'

  • 09 I I Principal Environmental Engineer 423 843-6700 05 11 I OF PRINCIPAL EXECUTIVE I I !YEAR I DAY I 1 OFFICER OR AUTHORIZED AGENT AREA I NUMBER MO -Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

___ _ ___ _20DD_'L: DAISY _ _Jf'U738'1._ ______ _ FacJl!!y _ _IYA PLANT _____ _ _!jAMIL TOJ'LCOUNTY._ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) TN0026450 . l _ _ _EERM[l NUMBEI MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT Form Approved. OMB No. 2040-0004 DAY 30 NO DISCHARGE I XX I *** NOTE: Read instructions before completinq this form. ioo4oo 1 o o !EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE '!00556 1 0 0 I EFFLUENT GROSS VALUE '!FLOW, IN CONDUIT OR THRU !TREATMENT PLANT PERI\i1.1T REQUIREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT

                • ******** RERM .. I_T_.**_:_-_
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                • ** **** DAlLY f CALCTD 15oo5o 1 o o EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL REQUIREMENT
  • REPORT MOAVG DAILy k ] "'{EEkl'i l' GRAB,< ******** *******.,.. ******** ********

19 I ... , __ .-... J GR 0 oss 0 VALUE **** 1 SAMPLE MEASUREMENT MG/L I 1 . . . RERMIT *r* T ----:;;::r.-. -. -I .. REQUIREMENT . *. . "* T c I ** : .. -, .. , * * '* !1 Certify under penalty of law that this document and all attachments were prepared under my r. \ _:) *. _ * ( TELEPHONE DATE or superv1s1on 1n accordance w1th a system des:gned to assure that qual1f1ed personnel , .j..f ( ilV.lC.-tL--L-t.l{ cl_![., ) ._:.( L {( properly gather and evaluate the Information submttted Based on my 1nqu1ry of the person or ...... 1 -"' persons who manage the system or those persons d:rectly respons:ble for gathenng the Principal Environmental Engineer 23 843 6700 09 05 11 . . . 1nformat:on the 1nformat1on subm:lled

s to the best of my knowledge and belief true, 4 -. Sequoyah Site V1ce Pres1dent accurate, and complete I am aware that there are s:gn:f1cant penalt1es for subm1111ng false SIGNATURE OF PRINCIPAL EXECUTIVE I _ . __J,nformat1on 1nclu01ng me poss:b1l1ty or f1ne and 1mpnsonment for know1ng V1olat1ons.

OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR! MO DAY TYPED OR PRINTED 1 CODE 1 COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Add res§_ ___E Q'"BOX.1_9QQ_ ___________ _ -__ DAISY _ ______ _ Facility _ _:s;JA PLANT _____ _ Locatio.!}_ .JjAMIL TOJ::LCOUNTY __________ _ [ C . -....... . TN0026450 ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING 110 T RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** NOTE: Read instructions before completinq this form. QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS EX OF I TYPE ANALYSIS i I _______ j IIC25 STATRE ?DAY CHR iCERIODAPHNIA <TRP3B 1 0 0 ]EFFLUENT GROSS VALUE f------- JIC25 STATRE ?DAY CHR IPIMEPHALES I ******** ******** ******** ******** k' ******** ******** !TRP6C 1 0 0 ]EFFLUENT GROSS VALUE . ":. ___ 4s.2 :'I<r MINIMUM' 1 I L *I I 'I -SAMPLE I I *. 8ERMlT* REQUIREMENT "t, < > 'C SAMPLE MEASUREMENT I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER II Certify under penalty of law that th1s document and all attachments were prepared under my : d1rect10n or supervis1on tn accordance w1th a system des1gned to assure that qualified personnel I Timothy P. Cleary ]properly gather and evaluate the 1nformat1on submitted. Based on my 1nqUify of the person or I !persons who manage the system. or those persons responsible for gathenng the 11nformat1on the 1nformat1on subm1tted IS to the best of my knowledge and bel1ef. true, I Sequoyah Site Vice President accurate, complete 1 am aware that there are slgn;f,cant penalt1es for subm1tt1ng false _ _____ _________ tmat1on, 1nclud1ng the poss;bility of f1ne and 1mpnsonment for know1ng violations I L_ TYPED __ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous may be used kpt K.U L-u 0

_J Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 23 TELEPHONE I DATE -----I I I' 423 843-6700 09 05 I 11 I I iYEAR AREA I NUMBER CODE 1 of 1 PERMITIEE NAME/ADDRESS

!Include Facilitv Name/Location 1f Different) ___ _ AddresL __£'_ ___________ _ -__ DAISY _ _ _;l738L ______ _ Fac_!l!!y_ ...2_VA ._g-:QUOYAH NUCLEAR PLANT _____ _ Locatio!]_ _!:!AMIL TO!:!_COUNT:f_ _________ _ ATIN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT (DMRJ TN0026450 116 G PERMIT NUMBER DISCHARGE NUMBER MONITQRING PERIOD YEAR I MO I DAY I I MO I DAY From 09 I 04 I 01 I Toj 09 I o4 I 30 MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT NO DISCHARGE CJ *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinQ this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. \FREQUENCY I SAMPLE 1 EX OF TYPE ANALYSIS AL I AVERAGE MAXIMUM UNITS MINIMUM AVERAGE I MAXIMUM UNITS 0 ******** rBRIS, FLOATING (SEVERITY)

                • ******** ******** 0 9A I 1 01345 1 0 0 OIL AND GREASE VISUAL VISUAL
  • VISUAL I I 84066 0 0 !EFFLUENT GROSS VALUE ( I 1 IVIV 1 v '"'._ 1 [ '[' v '.u--'"[ I . -* *--**. I i I SAMPLE I I . L I ME*A** .*

__ .*_*.T_* .. **.. *.** . I .. " .. '" ' .*...... II I . I .. .. l-_ ----- .. . .:--.£ §l__2j_ .. <*""-+-*--t---+.......:..._____:.__f.......:...__ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I' Cerl1fy .. Timothy P. Cleary Sequoyah Site Vice President persons informat1 accurate TYPED OR PRINTED penally of law lhalth1s document and all attachments were under my I .l' 1 j . . . ( *, j i . * *. or superv1s1on 1n accordance w1th a system des1gned to assure that qual1f1ed personnel .v*t.Q4) *l'. f\_.L.*,. ! *\ LA *. ;() .. ,(_ Q_ ., """""* .. ,, .... ,00 ,. .. , '" .. , '""""" "'"" oc .. who manage the system, or those persons responsible for gathenng the Principal Environmental Engineer on, the informat1on subm1tted 1s , to the best of my knowledge and bel1ef, true, , and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE on. 1nciud1ng the possibility of f1ne and 1mpnsonment for know1ng Violations OFFICER OR AUTHORIZED AGENT i COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used TELEPHONE DATE I 423 843-6700 09 1 I YEAR I M y CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Addres.L_ _f ___________ _ ___ -__ 20DD_'L-DAISY _ _Iri)738i_ ______ _ Facil1tv_ ..I:JA -..§EQUOYAH NUCLEAR PLANT _____ _ Locatio.!l_ .J+/-AMIL TO..t!._COUNTY._ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 PERMIT NUMBER ,-117-<[l MONITORING PERIOD MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 NO DISCHARGE [_] *** YEAR I DAY From I 09 To 30 ATTN: Stephanie A. Howard PARAMETER

DEBRIS, FLOATING (SEVERrTY)

I I 101345 1 0 0 SAMPLE MEASUREMENT QUANTITY OR LOADING AVERAGE MAXIMUM ******** ******** ******** ******** QUALITY OR CONCENTRATION UNITS MINIMUM AVERAGE MAXIMUM ******** ******** 0 IEFFLUENT GROSS VALUE , """. u-PERMIT REQUIREMENT 94 lOlL AND GREASE VISUAL ******** 0 84066 0 0 EFFLUENT GROSS VALUE UNITS 9A . '* :* PERMIT: . v * -i SAMPLE MEASUREMENT PERMIT REQUIREMENT

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER !I Cert1f f--.-.---d1rect1D Timothy P. Cleary Sequoyah Site Vice President r------------,=yp-ED OR PRINTED __ _ proper person 1nform accura mform ____j___ 'under penalty of law that th1s document and all attachments were prepared under my nor superviSIOn in accordance with a system designed to assure that qualified personnel y gather and evaluate the Information submitted.

Based on my inquiry of the person or ; who manage the system, or those persons directly respons1ble for gathenng the t1on, the informat1on submitted is , to the best of my knowledge and bel1ef, true, e, and complete l am aware that there are sign1f1cant penalties for submitting false t1on, 1nc!ud1ng the poss1bil1ty of fine and 1mpr1sonment for knowing violations COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 3/99) Previous edit1ons may be used (1 ij .d TELEPHONE DATE /.;; .p t l(,UL.Lt LA .CJ:.t.{/ULA. I 111 Principal Environmental Engineer 423 843-6700 09 05 I--SIGNATURE OF PRINCIPAL EXECUTIVE 1 AREA 1-OFFICER OR AUTHORIZED AGENT NUMBER I YEAR MO I DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Fac1litv Name/Location if Different) ___ _ Addres.L_ ___________ _ -__ __§_ODD-Y_- DAISY _ _IN 3738!_ ______ _ Facility _ _JyA-SEQUOYA_I::!_NUCLEAR PLANT _____ _ LocatioD_ _!:!AMILTO_ti_COUNT:I_ _________ _ ATIN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) I TN002645-0 I L_ PERMIT NUMBER ] 118 G DISCHARGE NUMBER MONITORING PERIOD YEAR DAY From 09 30 ******** MAJOR (SUBR 01) F-FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE [XX] *** Form Approved OMB No. 2040-0004 NOTE: Read instructions before completinq this form ******** 19 19 SAMPLE TYPE EFFLUENT GROSS VALUE **** :::1 <\!: *.. / ' :: <*; ;., oAtb?Mx MG/L :,. \(;RA8. * *:WEEK I SOLIDS, SETTLEABLE 00545 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT SAMPLE MEASUREMENT

                • ******** W'KW'W' ******** ******** 25 **** MLIL *J* .O .. NCE(c****]

GRA. B. I MONTH. * *. * .. ******** ******** ******** 03 1 50050 1 0 0 EFFLUENT GROSS VALUE . PERMIT REQUIREMENT

  • .

SAMPLE MEASUREMENT I I SAMPLE I I l I MEASUREMENT PERMIT REQUIREMENT -I I C NAMEmTLE PR,NC,eAC EXECUnVE OF"CER I"'" """'" "' Oo '""' '"" '"'"m'"' '"" "' "'""'m'"" ". repared under my I .\ , . . _.-, qJ' J TELEPHONE I DATE __j' I direction or superviSIOn In accordance With a system designed to assure that qua!rfled personnel '. ot*, n 'I. " .. , I ) *>-:. '\/.; ,. r-1, Timothy P. Cleary properly gather and evaluate the ;nformallon subm;tted Based on my inqwy of the person or I,_. '**"f 1.4.J UA.J \..,1\, L"-.A_.t.A I I I persons who manage the system, or those persons responsible for gathenng the Principal Environmental Engineer i i , . . . ;nformat;on. lhe ;nformat;on subm;tted

s. lo the best of my knowledge and bel;ef, true, _ *-_
:-----

423 843-6700 1 09 05 11 I I Sequoyah Site V1ce President accurate, and complete. I am aware that there are sigmf;cant penall;es for subm;ttmg false SIGNATURE OF PRINCIPAL EXECUTIVE I I I 11 yl 1 1 ;nformat;on, ;nclud;ng the poss;bll;ty of f;ne and ;mpnsonment for Know;ng v;olat;ons I OFFICER OR AUTHORIZED AGENT AREA 1 NUMBER 1 YEAR MO 1-My i TYPED OR PRINTED j CODE _ _[________ ______ l__ i COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfalL EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 S58 090611 800-NPDES CORRESPONDENCE June 11, 2009 State ofT ennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR MAY 2009 Enclosed is the May 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely, Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, LP 4B-C A. A. Ray, WT 7C-K G. R. Signer, WT 6A-K B. A. Wetzel, OPS 4A-SQN EDMS, WT CA-K (Enclosure) DMR0905.doc REVIEW/CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450 -MAY DMR ORGANIZATION: Chemistry and Environmental DOCUMENT PREPARED BY: Ann Hurt DATE: 06/04/2009 CATEGORY: NPDES Correspondence CONCURRENCES Name R c Signature -Comment Date v N Ann Hurt X u/7/o'J Stephanie A Howard X IP q oq Alan K Barringer X Debra J Bodine X Beth A. Wetzel X Chris R Church X Timothy P Cleary X (for signature) 'TfC.. I I INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. Tennessee Valley Authority. Post Office Box 2000. Soddy Daisy, Tennessee 37384-2000 June 11, 2009 State ofT ennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR MAY 2009 Enclosed is the May 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. P-. tleary I Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L _£'. ___________ _ -__ _20DD_.r_- DAISY _ _JN 37381.._ ______ _ Fac.!!!!Y_ -_g:QUOYAH NUCLEAR PLANT _____ _ 1Qcatio.!l._ .J:jAMILTOl:!_COUNTY __________ _ A TIN Stephanie A Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMR) TN0026450 i 101 G PERMIT NUMBEFL___j i YEAR I From [99 MONITORING PERIOD MO YEAR MO 05 To/ 09 I OS -R DAY 31 MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE [] *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. /FREQUENCY/ SAMPLE EX OF TYPE ANALYSIS jTEMPERATURE, WATER DEG. !CENTIGRADE looo10 z o o iiNSTREAM MONITORING !TEMPERATURE, WATER DEG. [CENTIGRADE

ooo1o 0 0 ;EFFLUENT GROSS VALUE irE_M_FI:oiF-F

.. BETWEEN s.ANii:>.-& juPSTRM DEG.C I SAMPLE I MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM UNITS MINIMUM ******** ********

                • ******** ******** ******** ********

1 s 0 EFFLUENT GROSS VALUE ... . ...*. ,u .. . r N PH [ <= I I I --I loo4oo 1 o o I !EFFLUENT GROSS VALUE E S, TOTAL SUSPENDED 100530 1 0 0 I I EFFLUENT GROSS VALUE !o!L.t.."No GREAsE iooss6 1 o o :*PERMIT.-*< <J;' ;*' '" SAMPLE MEASUREMENT PERMIT REQUIREMENT I SAMPLE 1 MEASUREMENT REO.UIREMENT

                • AVERAGE MAXIMUM ******** 23.8
                • 40.0 . DAII..YMX

2.1 UNITS

04 0 31 I 31 I MODELD SEE:i !CKREQ 04 RCORDR 04 I EFFLUENT GROSS VALUE IN CONDUIT OR THRU [TREATMENT PLANT *1.i.*k*: .. *r .. ,. .... _............. 1 r** **I -* R.coRDR jsooso 1 o o GROSS VALUE MGD I NAM EfTITLE PRINCIPAL EXECUTIVE OFFICER jl Cert1fy under penally of law that this documenl and all attachmenls were prepared under my -;direction or supervls1on in accordance w1th a system des1gned to assure that qual1f1ed personnel . Timothy P. Cleary jproperly gather and evaluale lhe Information submilted Based on my inquiry of the person or . . . '"'""' <M "" m "'""' """' "'"""" <m ""'""<< <M 1nformat10n the tnformat1on subm1tted IS to the best of my knowledge and bel1ef. true, Sequoyah Site Vice President . accurate, complele. I am aware lhal there are s1gn1f1can1 penall1es for submitting false *------1nformallon, 1ncludmg the poss1bil1ty of f1ne and 1mpnsonment for know1ng VIOlations 0 ED OR PRINTED 1 -------------**- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT No closed mode operation. The following information is included in an attachment:

1. CCW data 2. Veliger monitoring data EPA Form 3320-1 (REV 3/99)

TELEPHONE I DATE I I 423 843-6700 09 06 09 I AREA I NUMBER YEAR MO DAY CODE __j of 2 i DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 05/13/2009@ 1155 0.22 mg/1 05/14/2009@ 1644 CLS TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 05/13/2009@ 1200 <0.10 mg/1 05/14/09@ 1655 CLS TN EPA 8015 Mean# of Water Temp. Mean# of Water Temp. LOCATION SUB NOTES:% Sample Date ZM/m3 % Settlers ('C) Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY Clams/m3 ("C) Clam 11/21/2008 0 0 12 11/16/2008 230 12 lnplant RCW Dick Adcock 11/24/2008 31 0 11 11/24/2008 138 11 In plant RCW Dick Adcock 12/4/2008 0 0 10 12/04/2008 15 10 In plant RCW Dick Adcock 12/8/2008 0 0 9 12/08/2008 0 9 In plant RCW Dick Adcock 12/15/2008 0 0 9 12/1512008 0 9 lnplant RCW Dick Adcock 12/22/2008 0 0 9 12/22/2008 0 9 In plant RCW Dick Adcock 12/29/2008 0 0 9 12/2912008 0 9 In plant RCW Dick Adcock 01/09/2009 0 0 8 01/09/2009 0 8 lnplant RCW Dick Adcock 01/1612009 0 0 7 01116/2009 0 7 lnplant RCW Adcock I Bryant 01/22/2009 0 0 7 01/2212009 0 7 lnplant RCW Adcock I Bryant 01/30/2009 0 0 9 01/3012009 0 9 In plant RCW Adcock I Bryant 02/06/2009 0 0 6 02/06/2009 0 6 In plant RCW Adcock I Bryant 02/13/2009 0 0 11 02/13/2009 0 11 In plant RCW Love 02/20/2009 0 0 9 0212012009 0 9 lnplant RCW Bryant 02/26/2009 0 0 9 02/2612009 0 9 lnplant RCW Bryant 03/0612009 0 0 8 0310612009 0 8 lnplant RCW Dick Adcock 03113/2009 0 0 10 0311312009 0 10 lnplant RCW Dick Adcock 0312712009 0 0 14 0312712009 0 14 lnplant RCW Wayne Wright 03/30/2009 0 0 13 0313012009 0 13 lnplant RCW Dick Adcock 04/09/2009 28 0 14 0410912009 0 14 In plant RCW Dick Adcock 05/06/2009 880 24 19 05/06/2009 21 19 In plant RCW CNW 05/12/2009 15091 0.3 20 05/12/2009 47 20 In plant RCW CNW 05/19/2009 3961 2 20 05/19/2009 445 20 In plant RCW BJ 0512712009 7696 0.8 22 05/27/2009 457 22 In plant RCW CNW PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L ___£ Q_BOX_29Q_Q_ ___________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMR) TN0026450 101 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 -__ 20DD_1_-DAISY_ .....JN 3738+/-_ ______ _ Fac.!l!Jy_ JYA -_§E:QUOYAH NUCLEAR PLANT _____ _ Locatio.!]_ _l:!AMIL TO!:!._COUNT:I._ _________ _ PERMIT NUMBER DISCHARGE NUMBER MONITORING PERJOD DIFFUSER DISCHARGE EFFLUENT CJ ... From YEAR DAY 09 To 31 *** NO DISCHARGE ATTN: Stephanie A. Howard NOTE: Read instructions before completinq this form. _____ I NO. [FREQUENCY[ EX OF ANALYSIS I SAMPLE TYPE " !CHLORINE, TOTAL RESIDUAL ! lsoo6o 1 0 0 !EFFLUENT GROSS VALUE R=EMPERA TuRE".:-e:-:-RATE oF CHANGE ]82234 1 0 0 GROSS VALUE ' AVERAGE MAXIMUM UNITS MINIMUM AVERAGE ******** ******** ******** <0.018 PERMIT REQUIREMENT ! ' I I

                • I SAMPLE I MEASUREMENT I f?ERMIT . . REQUIRE.Ivl£f!i:l:

SAMPLE MEASUREMENT REQ:UIREMENT I I SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT I ... * ... *: I> I j l { '-;< , ,Yh$ SAMPLE MEASUREMENT MAXIMUM 0.042 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER II Cert1fy under penalty of law that th1s document and all attachments were prepared under my )¥. . U .<thz, , . d dlrect1on or superviSIOn 1n accordance wtth a system des1gned to assure that quallfted personnel {J_j*LU.,,. . ,UC(....-'-_ Timothy P. Cleary I properly gather and evaluate the tnformatton submttted Based on my tnqutry of the person or !persons who manage the system, or lhose persons responstble for gathenng the Principal Environmental Engineer the 1nformat1on subm1tted ts , to the best of my knowledge and belief, true, j ' Sequoyah Site Vice President jaccurate. complele 1 am aware lhat there are stgmftcant penalltesfor submittmg false

  • SIGNATURE OF PRINCIPAL EXECUTIVE

,_. --j'nformatton. 1nclud1ng the posstbil1ty of f1ne and tmpnsonment for knowtng vtolat1ons I OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) UNITS 19 0 27 I 31 GRAB MG/L ,_, *. , .*.. ., .. *'<PAY.'$." .* ' * ' .. 0 31 I 31 CALCTD I TELEPHONE DATE I 423 843-6700 09 t 06 09 i AREA I NUMBER IYEARL MO DAY CODE The following injections occured: 1. H-150M (max. calc. cone. was 0.0446mg1L--Iimit 0.050mgiL)

2. H-150M (low detection level analytical method was <0.020mgll--limit 0.050mgiL) 3/99) Previous editions may be used Page 2 of 2 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

___ _ Addres.§_ ...£_ Q_BOX_19QQ_ ___________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES! DISCHARGE MONITORING REPORT (OMR! TN0026450 101 T MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 -__ ___§ODDY-DAISY _ _JI'::l_]7381._ ______ _ PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facility_ JYA-_§_[:QUOYAH NUCLEAR PLANT _____ _ _hQcatiOD__ __!::!AMIL TOJ:!..COUNTY __________ _ EFFLUENT MONITORING PERIOD From 09 To 09 05 31 NO DISCHARGE LJ ... ATIN: Stephanie A. Howard YEAR I YEAR I MO DAy NOTE: Read instructions before completinq this form. PARAMETER QUALITY OR CONCENTRATION I NO. I FREQUENCY I SAMPLE EX OF TYPE QUANTITY OR LOADING iiC25 STATRE 7DAY CHR !CERIODAPHNIA iTRP3B 0 0 !EFFLUENT GROSS VALUE rC2ssTATRE-7oA.v !PIMEPHALES i iTRP6C 1 0 0 GROSS VALUE l I i I I SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQtJ]REMENJ J

  • AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM ******** ******** >100.0 ******** ******** -*********
                • L ' I r l

[ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I' Cert1fy under penalty of law that th1s document and all attachments were prepared under my * * --*--direction or superv1s1on 1n accordance w1th a system des1gned to assure that qualtfied personnel 1 Timothy P Cleary [properly gather and evaluate the Information subm1tted. Based on my inquiry of the person or persons who manage the system, or those persons directly respons1ble for gathenng the . . . J1nformation. the Information submitted 1s, to the best of my knowledge and belief, true, Sequoyah Site V1ce President accurate. and complete. 1 am aware that there are sign1f1cant penalties for submitting false 1nformat1on. 1ncludmg the poss1b11tty of f1ne and 1mpnsonment for knowing violations TYPED OR PRINTED i -COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled 5/10/09-5/15/09. Report is attached. EPA Form 3320-1 {REV 3/99) Previous editions may be used ..J O.<tlnucu-d Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT UNITS ANALYSIS 23 0 1 /180 I COMPOS ** $EE" fc;:'OMP{:[q: TELEPHONE DATE 423 843-6700 09 06 09 I AREA I NUMBER YEAR MO DAY CODE Page 1 of 1 June 9, 2009 Ruth Ann Hurt, SB 2A-SQN SEQUOY AH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, MAY, 2009 Per your request, I am only submitting an electronic copy of the subject report. The report provides results of compliance testing using fathead minnows and daphnids. Outfall101, samples collected May 10-15, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values for both species were> 1 00 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Call me at (256}386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment): Sherrard, R. M., PSC lX-C Files, ER&TA, CEB lB-M SQN May 2009m TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: June 9, 2009 1. Facility I Discharger: Sequoyah Nuclear Plant I TVA 2. County I State: Hamilton I Tennessee

3. NPDES Permit#: TN0026450
4. Type of Facility:

Nuclear-Fueled Electric Generating Plant 5. Design Flow (MOD): 1,579 6. Receiving Stream: Tennessee River CTRM 483.6) 7. 1Q10: 3,491 8. Outfall Tested: 101 9. Dates Sampled: May 10-15, 2009 10. Average Flow on Days Sampled (MOD): 1426, 1443, 1448 11. Pertinent Site Conditions: H-150M was injected into the ERCW B train beginning on 5/11/09@ 1220 through 5/14/09@ 1500. 12. Test Dates: May 12-19, 2009 13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids ( Ceriodaphnia dubia) 15. Concentrations Tested(%): OutfalllOl: 11.3,22.6,45.2, 72.6,100 Intake: 100.0 Pimephales promelas: UV treated Outfall I 01: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0 16. PermitLimitEndpoint(%): Outfall101: IC2 5=45.2% 17. Test Results: Outfall101: Pimephalespromelas: IC 25 > 100% Ceriodaphnia dubia: IC25 > 100% UV treated Outfall 101: Pimephales pro me las: IC2s > 100% Page 1 of 107

18. Facility Contact: Ann Hurt Phone#: (423) 843-6714 19. Consulting I Testing Lab: Environmental Testing Solutions, Inc. 20. Lab Contact: Jim Sumner Phone #: (828) 350-9364 21. TV A Contact: Cynthia L. Russell Phone #: (256) 386-2755 22. Notes: Outfall101 samples collected May 10-15,2009, showed no toxic effects to fathead minnows or daphnids.

The resulting IC 25 values, for both species, were > 100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Minnow survival was significantly reduced when exposed to intake samples during this study period. Page 2 of 107 Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. METHODS

SUMMARY

Samples: 1. Sampling Point: Outfall 1 01, Intake 2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (oC) (mg/L) First Used By_ 101 05-10-09 0859 to 05-11-09 1510 1.8, 1 .8t <0.10 05-12-09 0950 05-11-09 0759 05-13-09 0850 Intake 05-10-09 0933 to 05-11-09 1510 1.3 <0.10 05-12-09 0950 05-11-09 0833 05-13-09 0850 101 05-12-09 0821 to 05-13-09 1433 3.2, 2.ot <0.10 05-14-09 0900 05-13-09 0721 05-15-09 0853 Intake 05-12-09 0839 to 05-13-09 1433 2.1 <0.10 05-14-09 0900 05-13-09 0739 05-15-09 0853 05-14-09 0828 to 05-16-09 0900 101 05-15-09 1428 2.3, 1.5t <0.10 05-17-09 0920 05-15-09 0728 05-18-09 0903 05-14-09 0842 to 05-16-09 0900 Intake 05-15-09 1428 1.7 <0.10 05-17-09 0920 05-15-09 0742 05-18-09 0903 *TRC =Total Residual Chlonne tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrivaL 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath. Aliquots of Outfall 1 01 and Intake samples were UV -treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Page 3 of 107 Pimephales promelas Test Organisms:

1. Source: Aquatox, Inc. 2. Age: 20.33 hours old Test Method Summary: 1. Test Conditions:

Static, Renewal 2. Test Duration: 7 days 3. Control I Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

4 5. Organisms per Replicate: lQ 6. Test Initiation: (Date/Time) OutfalllOl 05-12-09 1220 ET UV Treated Outfall 1 01 05-12-09 1232 ET 7. Test Termination: (Date/Time) Outfall 101 05-19-09 1123 ET UV Treated Outfall 1 01 05-19-09 1140 ET 8. Test Temperature: Outfall 101: Mean= 24.7°C (24.2-25.1°C) Ceriodaphnia dubia In-house Cultures <24-hours old Static, Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic 10 l 05-12-09 0950 ET 05-19-09 0854 ET Mean= 24.9°C (24.6-25.2°C) Test Temperature: UV-Treated Outfall101: Mean= 24.7°C (24.3-25.0°C) 9. Physical I Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and exposure test solutions were analyzed daily for pH and dissolved oxygen. 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA). Page 4 of107 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1. Results of a Pimephales pro mel as Chronic/ 7 -day Toxicity Test. (Genus species) (Type I Duration) Conducted May 12 -19, 2009 using effluent from Outfall 1 01. Test Percent Surviving Solutions (time interval used-days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 98 98 98 98 98 22.6% 100 100 100 100 100 98 98 45.2% 100 100 100 100 98 98 98 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 98 98 98 98 Intake 100 100 90 68 60 53 50 Test Solutions Mean Dry Weight (mg) (%Effluent) (replicate number) 1 2 3 4 Mean Control 0.730 0.684 0.895 0.761 0.768 11.3% 0.753 0.655 0.723 0.717 0.712 22.6% 0.654 0.723 0.673 0.760 0.703 45.2% 0.646 0.684 0.638 0.649 0.654 72.6% 0.665 0.632 0.656 0.603 0.639 100.0% 0.723 0.604 0.700 0.641 0.667 Intake 0.212 0.285 0.496 0.567 0.390 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Pennit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso: TUc = 1001IC2s Page 5 of 107 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 12-19, 2009 using effluent from Outfall 101. Percent Surviving Test (time interval used-days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effluent) Data (replicate numbed 1 2 3 4 5 6 7 8 9 10 Mean Control 33 34 33 34 34 32 34 34 31 31 33.0 11.3% 33 36 32 32 36 33 33 34 33 32 33.4 22.6% 35 36 34 36 32 33 35 34 33 33 34.1 45.2% 36 34 31 35 35 33 33 34 33 31 33.5 72.6% 37 37 36 32 35 32 31 34 34 33 34.1 100.0% 39 38 32 36 34 33 34 35 34 31 34.6 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 1 00/LCso: TUc = 100/ IC2s Page 6 of 107 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 12-19, 2009 using water from Intake Percent Surviving Test (time interval used -days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effluent) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 33 35 33 35 34 33 31 31 33 33 33.1 Intake 39 35 41 36 35 38 37 36 37 34 36.8 IC2s Value: > 100% Calculated TU Estimates: < 1.0TUc* Permit Limit: N/A Permit Limit: N/ A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 1 00/LCso: TUc = 100/ IC2s Page 7 of 107 TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets) 3. Results of a Pimephales pro mel as Chronic/ 7 -day Toxicity Test. (Genus species) (Type I Duration) Conducted May 12-19, 2009 using effluent from UV Treated Outfa11101. Test Percent Surviving Solutions (time interval used-days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 98 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg) (% Effluent) replicate number} 1 2 3 4 Mean Control 0.637 0.743 0.695 0.659 0.684 11.3% 0.775 0.852 0.756 0.772 0.789 22.6% 0.737 0.727 0.706 0.653 0.706 45.2% 0.693 0.722 0.683 0.756 0.714 72.6% 0.682 0.668 0.665 0.722 0.684 100.0% 0.631 0.664 0.751 0.745 0.698 Intake 0.695 0.635 0.662 0.642 0.659 lC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LC 5 o: TUc = 100/ IC2s REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) Species Date Time Duration Toxicant Results (IC2s) Pimephales promelas May 12 -19, 2009 1208 7-days KCl 0.75 g/L Ceriodaphnia dubia May 05 -12, 2009 0905 7 days NaCl 1.07 g/L Page 8 of 107 PHYSICAL/CHEMICAL

SUMMARY

Test 1 "<. <::i ..::: §' .;:: -<::> .;:: §-""=l .s J Water Chemistry Mean Values and Ranges for P imephalespromelas and dubia Tests, treated SeqlJ()yalJ}'-l [l<.;]earPlant perfonned May 12-19,2009.

  • ___
    • : Outfull101 . ' .. .. , . . . . . . . ............. , ... , ............ , ..... . Sample ID rature (mg/L CaCOJ) Hardness Initial Total Residual Chlorine (mg/L) 24 7 Control 8 6. .... J ....

....... , ......... ........ , ........ ........ , ......... 7}S. ........ 1 ......... .......... , ............. ........... J ............. .......... t .......... , : 24.5 < 24.8 1 7.4 7.8 6.9 H 7.5 7.32 H H6 7.04 7.44_ 1_02 H 338 __§(}___j..L__(il_ .. 88 H 92 : ** ........ 24.7 11.3% 1*24.7.:>24.8 -z4.8 22.6% 7*7 *I* ?*r *t*** 7.-V****l********7}2 .......... ,. ?97***-****t* ....... , .. , .. .. .. 1 .... .. 24.2 *.-' 24.9 7.5 *,.: 8.0 6.9 H 7.5 7.26 H 7.50 7.01 *-: 7.36 284 :-:. 306 : *. .. --* 24.5 ****\******** 7Jf. **f****** J .. ..... 1 ........... , .. ,... . ... 1 .... . 24.2 25.1 7.5 :.: 8.1 6.8 H 7.5 7.26 H 7.49 7.03 :.: 7.34 268 H 287 : * -z4.8 45.2% 124 7 -24.9 -*-*-* 76 7.7 x:o I** ;;;;j} ;;; + N:** r* 72.6% 24.8 124 7 -24.9 7.7H**7.91*;;_;;tf?st7JZ.Y;;:;;;\;;9};?7.34j is9Y.8.2osl*****************:r TN*** --'-24.8 100.0% 124:7:>25.0 2 ...;:,_;_ 64 ....... +.*2 .. 4 .. } .... , .. 4 .. ,* .. 6 ... 2 .. 4_91 7.7 F7.sl6:8 H 7.5 + 7:1};-5\39 l 69/.:\\::z;f is6+ so ****f3 6sl6c 24.9 Intake 1**:;;*_6'\ 25.1 3 ......... .. *******1******* .. ......... ......... ......... ......... t .. ............ l ............. ? ................ l ......................... . 24.4 -24.7 7.7 :_: 7.9 6.s H 7.5 7.11 > 7.39 6.97:.! 7.2s 143 q 1s3 s2 :-57 6o :.; 64 <o.1o i-<o.1o ?.4 R Control : 24_7 jj_25.0 0 ******,*,*,*,* + ?5:'2 **l** 7?. , ,., ?.:?.,,,,, .L. ?.;?.? ... ,,, ?.}?.f , J No *'*'*'?J 24.8 -' 25.2 7.4 *-* 7.8 7.5 !-7.8 1 7.32 -: 7.66 7.21 :.: 7.47 302 i-: 338 [ 60 :

  • 61 89 88 92 11.3% 24.9 1*24 7*<***2s.o 22.6% I 247 ,-25.1 45.2 *;., I 2.4 9 .... 24 8 -25.1 72.6% I .. '24.8 :-25.1 1oo.o% f .. i4:s::r25.1 24.9 Intake I .......... , ... I . l4.8 __;..; 25.

.. .l .... ..?.?.. ........ , ......... ......... , ........ ......... , ......... ......... , ......... ........ . 24.8 .. 25.2 I 7.5 :.: 8.0 7.4 H _7.8 7.26 H 7.50 7.22 :.: 7.49 284 :-306 ?4? J 7.7 **I* 7.?..... ., ........ ........ 1 ....... .7}.9. ......... , ....... ..... [ ........... , ............... [ .... . 24.8 -25.21 7.s .: 8.1 7.4 H 7.7 7.26 H 7.491 7.22 :-: 7.48 268 H 287 2 5 ql** 7 7 *I .7. ......... 1 ......... 7;.3..7 ...... 1 ......... 7}8. ........ 1.. 24.8 -25.2 7.6 -s.o 7.4 :-7.7 7.23 H 7.47 7.22 .: 7.48 234 H 247 249.1. . .? **I*

  • I****** ..

....... 1 ........ 7,*3S. .... 1 ...... 1,?8. 24.7 -25.2 7.7 *-: 7.9 7.5 :

  • 7.7 7.18 H 7.46 7.22 :-: 7.42 189 ;. 205 I i *::** *:

..... *1 ......... 7.7. .......... 1 .......... 7.:? .......... 1 ......... 7 ,*.2,?. ........ 1********?:. 3 .. 1. ........ l*** ...... ........ ?.7. ............ 1 ......... 24.7 -' 25.1 7.7 *-: 7.8 7.5 H 7.7 7.14 H 7.39 7.20 < 7.38 145 H 156 so :-65 60 H 64 ...... J .... ... __ ..... *f* ..... ..?.:?._ ......... j***** .... .7}.5 ......... , ........ ........ _._[********} .. 1.?. ......... , .... _ ........ ............ , ..... _._ ..... N 24.6J.:l_25.1 I 7.7__i<J9 7.5 d 7.7 7.11 H 7.39 7.09 :-i 7.35 143 H 153 52 i-' 57 60 h 64 ----< 0.10 < 0.10 <0.10 <0.10 (9. * .. .. :: ....... :: .. :.*: .. :* :.:*::* .J::::::*::.t::*:::: ... * .. ::::.: ::::::*:::.: .,!.,::: .. ::::: .::* :::::::. :::::.:::

  • . ::* ... _:r .... Pimephales promelas 24.7 24.2 25.1 . i : : :

....... 24:9* . 6. is:.:2. . . :::* :: : ): ..... .. .... ....

.:::::::::::-
.. :::::. :::::::.*::

T_ ... :::::: :::: :::::* : :::::::::: .:*:: ::::.:::::. Page 9 of 107 .......................................................................................... [PHYSICAL/CHEMICAL

SUMMARY

..........................................
                                  • -*****

........................... -...................................................................... -.......................................... . Test ::: ;:: I:)., "" ..:::: ...:::: & ;: .;:: Q.., ........ .. .. .. .. 1.q1 ... performed May 12-19, 2009. i**r* * .. ************-r*-r**************

  • ***********-*-;***r*******
  • -****-r*********
    • r *:i***************:****************:****.***
                • -r*****-****
        . . .. . . '-
            • .
                                        • Sample ID Temperature Cq Dissolved Oxygen (mg/L) pH (S.U.) Conductance .

........................ Initial Final Initial Final Initial Final (p.mhos/cm) (mg/L CaC03) Control 24.8 24.6 7.7 7.5 7.44 7.27 314 60 I 7.5 > 7.8 7.28 ;_ 7.53 7.o4:J 7.44 296 :_ 334 59 :_: 61 24.7 -24.9 24.4 -24.9 7.0 :-7.8 11.3% 24.8 24.6 7.7 7.3 7.45 7.25 302 24.7) 25.0 24.4 -24.8 ....... 7.29 :_ 7.53 7.08.LT 7.38 282 rr 321 T T 7.5 :-7.9 6.8 !-7.5 22.6% 24.8 24.5 7.7 7.3 7.45 7.23 282 24>7 .. : :*: 24.9 24.4 *-' 24.7 ****7:5 ... [) .. 7:9 .. . . . . ' ' ' . . . . . . . . . . ' . . . . . . . . . .

      • 7:29L'7'.'54

7.02 , :r 7.39 ''''''''')f'''''''' 6.8 :-: 7.5 24.8 24.5 7.8 7.2 . 7.42 7.21 246 45.2% 1 .. 24 .. 7 .. .. 24.9 . . . . . . . . . ' . . . . . . . . . . . . . . . . . . ............................ 24.3 > 24.9 ****6:8'']) .. 7'.'4 ... 23'i .. j') .. 258 .. . ............ .. '1' . ' .......... ' 7.6 H 8.o 7.28 :-7.51 24.8 24.6 7.8 7.2 7.38 7.20 200 72.6% 24.3 _: 24.8 ***7:6 .... :*:-:-.. io .. . ' . . . . . . . . ' . . . . . . . . . . . . . . . . . ... .. 7'.'49 *

  • 7'.'o3' *: *: r
  • 7: 3'{i * **'is9 ..

... 2a7** ''"l_'f'''" 24.7 :_ 24.9 6.8 :-: 7.5 24.8 24.6 7.8 7.2 7.33 7.18 153 50 100.0% 24.7 _: 25.0 ... ............................... .. 7:43 .. '147**:*:*****'i'59

          • 44**

.. 24.3 _: 24.8 7.6 _: 8.0 6.8 :-: 7.5 Intake 24.9 24.6 7.8 7.2 7.30 7.14 149 53 24.7-250 24.4 -25.0 7.6 -: 7.9 6.8 ) 7.5 7.15< 7.43 6.s9:L:: 7.29 141 *c* 155 50 < 57 : .. .. , .. 24.7 Page 10 of 107 Hardness (mg/L CaC03) 91 88 ) 92 ': **:* .. f .. [ *: f .. :-.. : 62 60 <. 64 62 58 T_ 66

SUMMARY

I CONCLUSIONS Page 11 of 107 Outfall 101 samples collected May 10-15, 2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were> 100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Minnow survival was significantly reduced when exposed to intake samples during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment.

Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution. DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL 1. Pimephales promelas None 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None 2. Ceriodaphnia dubia None Page 12 of 107 PHYSICAL AND CHEMICAL METHODS 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2 .. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B. 4. Dissolved oxygen was measured by SM 4500 0 G. 5. The pH was measured by SM 4500 H+ B. 6. Conductance was measured by SM 2510 B. 7. Alkalinity was measured by SM 2320 B. 8. Total hardness was measured by SM 2340 C. 9. Total residual chlorine was measured by ORION Electrode Method 97-70. QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013.

Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC 25 values in giL KCl orNaCl. 2. Standard Toxicant:

Potassium Chloride (KCI crystalline) for Pimephales pro mel as. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia. 3. Dilution Water Used: Moderately hard synthetic water. 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses. Page 13 of 107 REFERENCES

1. NPDES Permit No. TN0026450.
2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002). 3. Standard Methods for the Examination of Water and Wastewater, 20th Edition, 1998. 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 14 of 107 Page 15 of 107 Sequoyah Nuclear Plant Biomonitoring May 12-19, 2009 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, Page 16 of 107 Date 03/12/1998 } 03/13/1998

      • 03/14/1998
03/15/1998

.** 03/16/1998 03117/1998 03/18/1998 09/08/1998 09/09/1998 09/10/1998 0911111998 09112/1998 9/13/1998 09/14/1998 March 12, 1998-May 15, 2009 PCL-401 mg!L Copolymer 0.005 0.011 0.021 0.019 0.015 0.015 0.015 0.024 0.024 0.024 0.024 0.024 0.024 0.023 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, Page 17 of 107 Date 11/25/2001 11/26/2001 11/27/2001 11/28/2001 11/29/2001 ..* 11/30/2001 .. 12/09/2001 .* 12/10/2001 .*. *. 12/11/2001 .* 12/12/2001 . 12/13/2001

  • 12/14/2001
  • .. * *.. March 12, 1998-May 15, 2009 PCL-401 mg!L Copolymer 0.009 0.028 0.009 0.009 0.009 0.009 0.009 0.019 0.019 0.018 0.019 0.019 0.019 0.019 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.021 0.021 0.021 0.020 0.021 0.020 0.02 0.019 0.019 0.02 0.02 0.02 0.02 0.02 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 1 01) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, Date 01/02/2002 01/03/2002 01/04/2002 01/05/2002 01/06/2002 01/07/2002 02/24/2002 02/25/2002 02/26/2002 . 02/27/2002 02/28/2002 . 03/01/2002

.* 05/05/2002

05/06/2002 05/07/2002 05/08/2002 05/09/2002 05/10/2002 08/04/2002 . 08/05/2002 08/06/2002 .*. 08/07/2002

    • .*.**. ** 08/08/2002

\ ... 08/09/2002

  • 10/06/2002
  • .*.. 10/07/2002
  • .. 1 0/0 8/2 002 !:..*< ************
                  • c:

.. *j****** 10/09/2002 10/10/2002 .*.. 10/11/2002 .*.. 01/12/2003 ... 01/13/2003 01114/2003

  • .** 01/15/2003 01/16/2003

.. 01/17/2003

. 04/06/2003

.*. 04/07/2003 . . 04/08/2003

  • ..
  • 04/09/2003 . 04/10/2003 04/ll/2003 . Page 18 of 107 ************************

March 12, 1998-May 15, 2009 PCL-401 mg/L Copolymer 0.02 0.014 0.014 0.014 0.023 0.023 0.023 0.008 0.008 0.02 0.02 0.019 0.02 0.019 0.018 0.018 0.019 0.019 0.018 0.018 0.018 0.018 0.018 0.018 0.019 0.020 0.020 0.020 0.009 0.021 0.021 0.021 0.021 0.022 Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, Date 06/15/2003 06116/2003 06/17/2003 06/18/2003 06/19/2003 06/20/2003 08/03/2003 08/04/2003 08/05/2003 08/06/2003 08/07/2003

08/08/2003 10/05/2003 10/06/2003 10/07/2003

10/08/2003 10/09/2003 10/10/2003 02/01/2004 02/02/2004

02/03/2004 02/04/2004 02/05/2004 02/06/2004 05/04/2004 . 05/05/2004

  • ... 05/06/2004 05/07/2004
  • 05/08/2004
    • .** .. 05/09/2004
  • ..... Page 19 of 107 07/04/2004 . 07/05/2004

07/06/2004 07/07/2004 . 07/08/2004 r*\********************* o7/09/2oo4

n. \ .. ?/ ?<> N 0 ...... Q ....... BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 ofl Client: TVA Environmental Testing Solution, Inc . Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify):

Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comments(} Chevy Williams: NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell : ...{.1 _ _,. / Adam Deimling: ./ L.5x -Collected By: Chevy Williams, Ben Mitchell, Adam Deimling Fax: 828-350-9368 Metals Analyzed. Samples remained oiilce through out sampling and transport to lab. Field Identification I Grab/Comp. Collection Dateffime Container Flow ptmJul i. 544'-f Sample Description Number& MGD Rain Event? Volume (Mark as Appropriate) Laboratory Use Collected " . .. / .* .. ::<,.' .* Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-I* **** .* ...*... (EST) l**c:* :** Inches Number Temp. t"' ance * ... * ... ** ' >< .. :'*' . .:.*'.: > **.. . ,* P' .. ** . :;:> .. < *.*. ,* ***c (oC) SQN-101-TOX Comp 05/10/09-05/11/09 0859-2 (2.5gal) 14.)1, v O.D( '(. 6\0 0759 SQN-INT-TOX Comp 05/10/09-05/11/09 0933-1 (2.5 gal) NA J 0.ol \.!)(_ lS'O "*' 0833 Sample Custody -Fill In From Top Down 1f. Relinquished By (Signature): Date/Time Received By (Signature): I. cv.. 1tJ ... ..... 0 05/11/09 /tJRtGsr Sonic Delivery 05/ll/09 131<-. C\ "' Sonic Delivery /31<.. SLJ*f 4 05/11/09 ETS 05/11109 3: I D Ptl\ £:\ \S\0 t\ '-'I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. IJQ It> N Vl 0 '""l ..... 0 -.1 BIOMONITORING CHAIN OF CUSTODY RECORD Page 1oft Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery -, Facility Sampled: Sequoyah NP 28801 General j J_ J Chevy Wllhams: NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell :-.2"'i IL Adam Deimling: y;;: Collected By: Chevy Williams, Ben Mitchell, Adam Deimling Fax: 828-350-9368 Metals Analyzed. Samples remained on ice through out sampling and transport to lab. Field Identification I Grab/Comp. Collection Daten'ime Container Flow '544Y Sample Description Number& MOD Rain Event? Laboratory Use Volume (Mark as Appropriate) Collected

    • ..*. ***.*** . . *.** .: *****< . *.*** **: ... ::. ...... Date Time Yes If Yes, No Trace ETSLog Arrival By Time Appear* (EST) *., : :*:*c.? l,x.=rz)!;

Inches Number Temp. E' ance '

  • 'C0 ; __ ,, ' ... *:*.ci ; < ' c*c) SQN-101-TOX Comp 05/12/09-05/13/09 0821 -2 (2.5gal) v 0"1:b\3.00 3."1 .. ;2.0"t J 0721 SQN-INT-TOX Comp 05/12/09-05113/09 0839-1 (2.5 gal) NA v

\"l. d\

  • 0739 2. Sample Custody Fill In From Top Down i--Woo£.. ..... .,_...vf . ..c. Relinquished By (Signature):

Date/Time Received By (Signature): '-Date/Time Chevy WimazruC4 05/13/09 10 55 6srr 05/13/09 C)l Sonic Delivery j3/t ;0:33 'E,\ Sonic Delivery fJ_ fl _U4 05/13/09 05/13/09 1_: :is PM E\ E:\ '-../ I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not anive until the following Monday. ---; i l.P,_ :;:,, r&-+' llol (JQ N 0'1 0 _, ..... Q -...:a BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 I Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): i Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 Phone: 828-350-9364 NPDES Number: TN0026450 B*n M>tchell ' : Adam Deirnling: Collected By Chevy Williams, Ben Mitchell, Adam Deimling Fax: 828-350-9368 Metals samples taken. Samples remained on ice through out sampling and transport to lab. Field Identification I Grab/Comp. Collection Dateffime Container Flow Sample Description Number& MGD Rain Event? Laboratory Use Volume (Mark as Appropriate) Collected ,'< Date Time ' .. Yes If Yes, No Trace ETS Log Arrival By Time Appear-" ........ , Inches Number Temp. MCe E:\ f:l . .._ > ' ' :.*'--("C) SQN-101-TOX Comp 05/14/09-05/15/09 0828-2 (2.5gal) 14-4<3:3 / o.olt J j,.. 0728 SQN-INT-TOX Comp 05114/09-05/15/09 0842-I (2.5 gal) NA v O.DL\ 01U51S.D3 \:l"L a\ 1k 0742 Sample Custody-Fill In From Top Down -i. < 11-_A .,. I Allrn .AKd.. Relinquished By (Signature): Date/Time Received By (Signature): ,_. -D !Time ..M. .......... ("] Chevy Williams 05115109 !Oo117sr Sonic Delivery 05/15/09 B!< f)q_ E.\ ' I Sonic Delivery{? tt> 05/15/09 ETS 05/15/09 !:2; 2 r fl'\ E. 'T" t'luf \...J v Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. ---

I I I I I 1 I I j I j j -I. l I ! l I ' 1 ! : I ! I I Page 1 of6 EnYitonmentilflating SoluUans, lilt. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: TVA Facility: Seguoyah Nuclear Plant NPDES #: TN 0026450 Project #: Dilution preparation information: Dilution prep(%) 11.3 22.6 Effluent volume (mL) 282.5 565 Diluent volume (mL) 2217.5 1935 Total volume (mL) 2500 2500 Test organism information: 45.2 1130 1370 2500 Organism age: "2o * \I.Quv'l':> Date and times organisms \-o'\ \loCO were born between: 72.6 100 1815 2500 685 0 2500 2500 Organism source: fl>..'Th'l-p P C'S* \\*d'\ Transfer bowl information: pH= SU Temperature = oc '"t'-t.l..:. Average transfer volume: o. 1.:!:.oS Daily feeding and. renewal information: Day Date Morning feeding Afternoon feeding 0 2 3 4 5 6 7 Page 27 of 107 County: Hamilton Treatment: Non-treated Outfall: 101 Comments: Test inf!Jrmation: Randomizin__g tem_Q}ate: nu'l Incubator number and 6C.. shelf location: Artemia CHM number: Date I Time in: *\"'*o'\ J'l.()b Date I Time out: OS*lJ:I*cf ll."tt Total drying time: Initial oven temperature: Final oven tem_Qerature: Sample MHSW numbers used batch used ) . Page 2 of6 EnvlrontntAt11Testtng.$olutloru,lnc. Species: Pimephales promelas Date: Client: TVA I Sequoyah Nuclear Plant-Non-treated Survival and Growth Data Day t.;UN.fROL 11.3% 22.6% A B c D E F -G 8 I J K L 0 ID 10 ID IO 10 /0 10 10 ID /Q 10 /D 1 ro 10 ID IO to 10 IO IO IQ /0 IO 10 2 /Q ID ro 10 /f) IO 10 1(:) lf::J fQ /(:) tO 3 JD lt> 10 10 LO ltl /0 10 lO 10 I() 4 10 10 10 lO 10 tD 10 10 IO 10 10 5 ID 10 It> /6 /I) q lb 10 10 10 10 10* 6 lo 10 lC) /0 (C) /(J 10 /() '\ ,f\fl /0 ID 7 '\ ID ID to /0 /0 /0 /0 10 /(J ro A= Pan weight (mg) Tray color code:: pl .u.. Analyst: 1"'1..1"' (1"-')..) 14-St IS". or 1;).. 4o 14.1)0 J3.6i )Lj, $'0 13.-:>.1 \3\'\0 Date: b S" *I}* b" B-Pan+ Larvae weight *'* )0 (mg) .f\l".(,r 'J.i>. tO )0.0(

}.D. d-S lo.ql &+ J.o.l/S" j.O.$D ()./.86 Analyst
Date:

C =Larvae weight (mg) =B-A 11-'?>C (, .l t.'\S 1. fo \ 1-S:?> t,.SS 1. 1.:?> 1.11 , .(,.t> Weight per initial number ' of larvae (mg) "" ",{? "'? = C I Initial number of larvae I'-.; '10 " " 1\ 'lS \o tf t>* \)' o* 0' \)' o* t>* (;)' Average Percent weight per reduction &.S7o initial from control 0.1 ... ().1\"2.. 1* 4. '1. 0.1 0-2> number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 28 of 107 .ETS

  • i) . . . J . ! -,j Enwlroam*ntal TaUnv So1utlont.lnc.

Page 3 of6 Species: Pimephales promelas Date: __ _ Client: TVA I Sequoyab Nuclear Plant-Non-treated Survival and Growth Data Day 45.2% 72.6% 100% M N 0 p Q R s T u v w X 0 /D /0 {0 10 10 10 10 lO /0 10 IC 10 l JO 10 to 10 l() ID 10 10 IO ID /0 (0 2 JD 10 10 /0 10 10 ro IO tO 10 tD 3 ID tc lD {{:) {() lD 10 10 10 10 /Q 10 4 10 IO /0 10 to ro 10 ,fA\"(:: *. 10 to ID 10 q 5 /0 I'C> t:\,d,fi 10 lO I () tO 10 10 Cj 10 tO 6 It> 10 " 10 (0 IO ID IC LC q I() 10 7 \SI'\ IQ 10 q I{) 10 /() ID 10 10 q 10 10 A= Pan weight (mg) G'f't. \ Tray color code:: p\ .. r-l3. %'\ J),ql 13.1-0 14.oi 13.1-J J 5'.15 /1.{.';)4 l4.l3 13.&q Analyst: f"\L.r-[......,_) Date: 1').-01\ B =Pan + Larvae weight (mg) -=--J.0.3S'

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ll.Or ;}D.80 'J.t;. 3 J JJ..n J,l. q I Analyst: (V\ L Date: ... C =Larvae weight (mg) =B-A
c. .

". lo. '5\o (p. lo (..'{I 1.0() Weight per initial number .).\f) "':.::>.. ,} h) '\I 0' rv"' of larvae (mg) if 'D = C I Initial number of larvae '\o '-0 '\II "" '-II 'o \ij '-0 " o* '0 c* ()' o' t;)' ()' t)* \Y o* \:)" ()' o* Average Percent weight per reduction

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7. o.\Diol \ \ number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed:

A Comments: Page 29 of 107 i) -=... r. s.,> ' . Page 4 of6 Envlronmmt*ITtstfng SGhJikms.lrK. Species: Pimephales promelas Date: _0:.:::5...__,_*1_,_"2_,* Client: TVA I Sequoyah Nuclear Plant-Non-treated Survival and Growth Data Day 100% Intake y z AA BB 0 10 ro 10 10 1 /(J 10 10 10 2 to [)10 3 lD 10 4 5 L\ U\F ir" 'l 10 6 '--\ ,c.lF lo 7 0 i-f \o llW' A= Pan weight (mg) ( h*c Tray color code:: pl.,,._ j::J lllq IY.l'i Analyst: /V'Lt" ll"*l) Date: r B =Pan+ Larvae weight (mg) f"'l.r' JS.ql lb. 11./b Analyst: Date: D'r C =Larvae weight (mg) =B-A 'Z.\"t "2..,85 t..\. '1\ ,S.Io 1 Weight per initial number ,..,t> 0 of larvae (mg) \. = C I Initial number of larvae '1,; .X '=' o* c* c* Average Percent weight per reduction -'\C1. 7. 7. initial from control number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: _t_ Page 30 of 1 07

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Inc Coauatratioa (%) Repliac.e hitiaiDD.mberof Fiaal a umber of A'"" Pa.a weight larv11c larvae (") A 10 10 14.87 Control B 10 10 13 86 c 10 10 15 01 D 10 10 12 40 E 10 10 14.50 11.3% F 10 9 13.70 G 10 10 13.68 H 10 10 14.50 I 10 10 13.91 22.6'Vt J 10 9 13.27 K 10 10 15.13 L 10 10 13.90 M 10 10 l3S9 45.2% N 10 10 13.97 0 10 9 13 70 p 10 10 14.08 Q 10 10 1372 '72.6o/o R 10 10 15.75 s 10 10 14.24 T 10 10 14 28 u 10 10 14 94 v 10 9 15 87 100 1% w 10 10 14 33 X 10 10 13.69 y 10 3 13.79 JOO'/* Jnmke z 10 4 14.14 AA 10 6 14.85 BB 10 7 13.49 OutfaliiOl: MSD-Dunnett's MSD value: 0.0890 PMSD-PMSO: 11.6 In tal<<: Dunnett's MSD value: 0.1860 PMSO: l4.2 TVA I Sequoyah Nuclear Plant, OutfalllOl Non-treated May 12-19,2009 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Not for Compliance Assessment, Internal Laboratory QC B-ha+l...arue Larvae weigbt (mg) Weigllt/ Survivia, Malawc::i.ptl Codllideat ofvariatioo Wcipt/ Jaitial .. mber weigb t ( rng} *A-B umber of larvae (Mg.) Swnrivi*C aumber or (Mft*WacJiolpa .... YiYia' afbln .. (mg) larv .. (mg} naberafl*"*e>("!.) 22.17 7.30 0.730 0730 20 70 6.84 0.684 0.768 11.8 0.684 23 96 8 95 0&95 0.&95 20.01 7.61 0.761 0.761 22.03 7.53 0.753 0.753 20.25 6.55 0.728 0.730 2.2 0.655 20.91 7.23 0.723 0.723 21.67 7.17 0.717 0.717 20.45 6.54 0 654 0.654 20.50 7.23 0.803 0.723 9.8 0.723 21.86 6.73 0.673 0.673 21.50 7.60 0.760 0.760 20.35 6.46 0.646 0.646 20.81 6.84 0.684 0.672 4.5 0.684 20.08 6.38 0.709 0.638 20.57 6.49 0.649 0.649 20 37 6.65 0.665 0.665 22.07 6.32 0 632 0.639 4.3 0.632 20.80 6.56 0 656 0.656 20 31 6.03 0 603 0.603 22 17 7.23 0.723 0.723 21 91 6.04 0.671 0 684 5.2 0 604 21 33 7.00 0.700 0 700 20 10 6.41 0.641 0 641 15 91 2.12 0.707 0212 16.99 2.85 0.712 0.764 8.3 0.285 19 81 4 96 0.827 0.496 19.16 5.67 0.&10 0 567 Minimum Significant Difference Percen1 Minimum Significant Difference Project oumber* 5444 --.. ------* -J r:rv""" .. ( J Mc-.auannival Meuweipt/ ""*eddlck.otol rod.adioa &0111 (Yo) l*itial aamber at' variaD.oa (M-wnp1 COII.lTOt(o/*} llllVIOC(mg) pwlllid .. au.beroftarne) % 100.0 0.768 11.8 Not applicable 97.5 Cl.71l 5.8 7.l 97.5 Cl.703 6.9 8.5 97.!1 0.654 3.1 14.8 lOCI.O Cl.639 4.3 16.7 97.5 0.667 8.2 13.1 50.0 0.390 43.2 49.1 PMSD tS a measure oftest precision. The PMSD is the minimum percent d1fference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test Lower PMSD bound determined by USEPA (lOth percentile) 12%. Upper PMSD bound determined by USEPA (90th percentile)- 30%. Lower and upper PMSD bounds were detenruned from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200la; USEPA, 200lb). USEPA 200 I a, 200 lb. Fmal Report. Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-{) l-{)04 and EPA-821-B-0 1-{)05 US Environmental Protection Agency, Ctncmnati, OH i "'C 1:1.? [JCI w N 0 _, ..... 0 -..J .ETS TV A I Sequoyah Nuclear Plant, Outfall101 Non-treated

  • * @lJ u _.,.,,. ____ . *(*, *'*** . Environmental Testing Solutions, Inc. May 12-19, 2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Da}': Growth Start Date: 5/12/2009 TestiD: PpFRCR Sample ill: End Date: 5/19/2009 Lab ID: ETS-Envir.

Testing Sol. Sample Type: Sample Date: Protocol: FWCHR-EPA-821-R-02-0 13 Test Species: Comments: Cone-% l 2 3 4 D-Control 07300 0.6840 0.8950 0.7610 11.3 0.7530 0.6550 0.7230 0.7170 22.6 0.6540 0.7230 0.6730 0.7600 45.2 0.6460 0.6840 0.6380 0.6490 72.6 0.6650 0.6320 0.6560 0.6030 100 0.7230 0.6040 0.7000 0.6410 Intake 0.2120 0.2850 0.4960 0.5670 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat D-Control 0.7675 1.0000 0.7675 0.6840 0.8950 11.817 4 11.3 0.7120 0.9277 0.7120 0.6550 0.7530 5.777 4 1.502 22.6 0.7025 0.9153 0.7025 0.6540 0.7600 6.851 4 1.760 *45.2 0.6543 0.8524 0.6543 0.6380 0.6840 3.113 4 3.066 *72.6 0.6390 0.8326 0.6390 0.6030 0.6650 4.343 4 3.479 *100 0.6670 0.8691 0.6670 0.6040 0.7230 8.152 4 2.721 Intake 0.3900 0.5081 0.3900 0.2120 0.5670 43.230 4 Auxiliary Tests Statistic Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97087646 Bartlett's Test indicates equal variances (p = 0.23) 6.92620468 Hypothesis Test (!-tail, 0.05)__ NOEC LOEC ChV TU MSDu MSDp Dunnett's Test 22.6 45.2 31.9612265 4.42477876 0.08902763 0.11599692 Treatments vs D-Control Point !COS* ICIO IC15 IC20 !C25 IC40 lC50 % 7.813 28.104 >100 >100 >100 >100 >100 SD 95% CL(Exp)

  • indicates IC estimate less than the lowest concentration Linear Interpolation (200 Resamp1es)

Skew TVA I Sequoyah Nuclear Plant, Outfall 101 DMR-Discharge Monitoring Report PP-Pimephales promelas !-Tailed Isotonic Critical MSD Mean N-Mean 0.7675 1.0000 2.410 0.0890 0.7120 0.9277 2.410 0.0890 0.7025 0.9153 2.410 0.0890 0.6543 0.8524 2.410 0.0890 0.6530 0.8508 2.410 0.0890 0.6530 0.8508 Critical Skew Kurt 0.884 0.63537989 1.21808683 15.0862722 MSB MSE F-Prob df 0.00884297 0.00272926 0.0292745 5, 18 sqn101_05-12-09data ""'C (10 (p "" 0 ...., -0 ...... TV A I Sequoyah Nuclear Plant, Outfall101 -Intake Non-treated Environmental Testing Solutions, Inc. Start Date: 5112!2009 End Date: 5119/2009 Sample Date: Comments: Cone-% 2 D-Control 1.0000 1.0000 11.3 1.0000 0.9000 22.6 1.0000 0.9000 45.2 1.0000 1.0000 72.6 1.0000 1.0000 100 1.0000 0.9000 Intake 0.3000 0.4000 Cone-% Mean N-Mean D-Control 1.0000 1.0000 11.3 0.9750 0.9750 22.6 0.9750 0.9750 45.2 0.9750 0.9750 72.6 1.0000 1.0000 100 0.9750 0.9750 *Intake 0.5000 0.5000 Tests Test ID: Lab 10: Protocol: 3 1.0000 1.0000 1.0000 0.9000 1.0000 1.0000 0.6000 Mean 1.4120 1.3713 1.3713 1.3713 1.4120 1.3713 0.7854 May 12-19,2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival PpFRCR Sample ID: ETS-Envir. Testing Sol. Sample Type: FWCHR-EPA-821-R-02-0 13 Test Species: 4 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 0.7000 Transform: Arcsin Sguare Root Min Max CV% 1.4120 1.4120 0.000 1.2490 1.4120 5.942 1.2490 1.4120 5.942 1.2490 1.4120 5.942 1.4120 1.4120 0.000 1.2490 1.4120 5.942 0.5796 0.9912 23.814 N 4 4 4 4 4 4 4 Statistic t-Stat 6.701 Shapii:o-Wilk's Test indicates normal distribution (p > 0.01) 0.93208891 Eguali!}: of variance cannot be confirmed Test (1-tail, 0.05) MSDu MSDp Heteroscedastic t Test indicates significant differences 0.11179766 0.11466427 Treatments vs D-Control TV A I Sequoyah Nuclear Plant, Outfall 101 -Intake DMR-Discharge Monitoring Report PP-Pimephales promelas 1-Tailed Critical MSD 2.353 0.2201 Critical Skew 0.749 3.38354E-16 MSB MSE F-Prob 0.78530011 0.01749093 5.4E-04 Kurt 0.88161324 df l, 6 sqnl 01_05-12-09data (1Q (t> (.;J ..... 0 """l ....... 0 -..1 TV A I Sequoyah Nuclear Plant, Outfall101 -Intake Non-treated May 12-19,2009 ! E: .. .. T;**-< S .. _ ... __ '0 .. ** *.*' Statistical Analyses Environmental Testing Solutions, Inc. Larval Fish Growth and Survival Test-7 Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: Comments: Cone-% I 2 3 4 D-Control 0.7300 0.6840 0.8950 0.7610 11.3 0.7530 0.6550 0.7230 0.7I70 22.6 0.6540 0.7230 0.6730 0.7600 45.2 0.6460 0.6840 0.6380 0.6490 72.6 0.6650 0.6320 0.6560 0.6030 100 0.7230 0.6040 0.7000 0.6410 Intake 0.2120 0.2850 0.4960 0.5670 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat D-Control 0.7675 1.0000 0.7675 0.6840 0.8950 11.8I7 4 11.3 0.7120 0.9277 0.7120 0.6550 0.7530 5.777 4 22.6 0.7025 0.9153 0.7025 0.6540 0.7600 6.851 4 45.2 0.6543 0.8524 0.6543 0.6380 0.6840 3.113 4 72.6 0.6390 0.8326 0.6390 0.6030 0.6650 4.343 4 100 0.6670 0.8691 0.6670 0.6040 0.7230 8.152 4 *Intake 0.3900 0.508I 0.3900 0.2120 0.5670 43.230 4 3.944 Auxiliary Tests Statistic Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94991392 F-Test indicates equal variances (p = 0.34) 3.45560646 Hypothesis Test (1-tail, 0.05) MSDu MSDp Homoscedastic t Test indicates significant differences 0.18600391 0.24235037 Treatments vs D-Control TV A I Sequoyah Nuclear Plant, Outfall I 0 I DMR-Discharge Monitoring Report PP-Pimephales promelas !-Tailed Critical 1.943 Critical 0.749 47.4672279 MSB MSD 0.1860 MSE Skew Kurt 0.13317371 -1.3590747 F-Prob df 0.2850125 0.01832517 0.00759199 1, 6 sqnl01_05-12-09data (10 (tl tJl i. * *

  • t___) Environmental Testing Solutions, Inc. Control 11.3% 22.6% 45.2% 72.6% 100% TVA I Sequoyah Nuclear Plant, Outfall101-Non-treated May 12-19, 2009 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses !'i444 Projec:t number:

Reviewed by: .J EnvlronmmtllludngSoh&ttons.lnt. Page 5 of6 Species: Pimephales promelas Client: TV A I Sequoyah Nuclear Plant-Non-treated 11.3% 22.6% 45.2% 72.6% 100% 100% Intake Page 36 of 107 Species: Pimephales promelas Client: TV A I Sequoyah Nuclear Plant-Non-treated Coocen-Parameter tration 11.3% 22.6% 45.2% 72.6% 100% lOOo/e Intake Page 37 of 107 Page 6 of6 Date: cS* 1-z. =o'1

Page 1 of7 .J Enwlronm.nWTastlng Solutlom.lne. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA Facility: Seguoyab Nuclear Plant NPDES #: TN 0026450 Project#: ____ _ Dilution preparation information: Dilution prep(%) 11.3 22.6 Effluent volume (mL) 282.5 565 Diluent volume (mL) 2217.5 1935 Total volume (mL) 2500 2500 Test orKanism source information: 45.2 72.6 100 1130 1815 2500 1370 685 0 2500 2500 2500 Organism age: < 24-hours old Date and times organisms were born OS* l"l.*cf\ ""tO CAt.\ 0 between: Culture board: 05*0")yt:1\. A Replicate number: 1 I z I 3 1 4 1 s 1 6 1 1 1 s 1 9 1 10 Culture board cup number: I IOI\CS.II'ol 1'"\l\'\ l1..1.l'l.'!ti1.S Transfer bowl information: pH-su Temperature= "Z.'-l.'\ oc Daily renewal information: Day Date 0 2 3 4 5 6 7 Control information: % of Male Adults: %Adults having 3ra Broods: % Mortality: Test initiation, 1 2 07. 07. ;oo7. IOQ7. 07. 01. MHSW batch used Acceptance criteria !>20% !>20% County: Hamilton Treatment: Non-treated Outfall: 101 Comments: Test information: Randomizing template color: l&l'\E> Incubator number and shelf location: YWTbatch: Selenastrum batch: Sample numbers used 2&\ ol.\*zt-<A -C:>'\* tt\ Analyst Summary of test endpoints: 7-day LCSO -, ICOi. NOEC 1 ao'. LOEC > mc*r. Mean Offspring/Female: 15.0 offspring/female ChV '>tocr. %CV: '4. \7 <40.0% IC25 ) Page 38 of 107

Page 2 of7 ..) Env!ronrrwntM Solutlont, Inc. Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant-Non-treated Date: CONTROL-I Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L-L \..._ \...._ '-'-'-L 1._ '-2 Young produced [) 0 0 0 0 0 () () a 0 Adult mortality L '-'--'--'-\.,_ \...._ '---'-\.,_ 3 Young produced 0 D D a D 0 a 0 0 0 Adult mortality L '--\_ \...._. '--L '--\,._.. L-L 4 Young produced '-\ '-l -::; $ 'S s '-\ '-\ Adult mortality '-'-L-'-'-'-L L \._ '-5 Young produced \0::, ,::, \::, \L.... \\ \'-l \L \"L \L. Adult mortality L. L \....... '--\._ '-L-'---\,__ '--6 Young produced 0 0 0 0 0 0 a 0 0 0 Adult mortality '-\.._ L '--'--L \.._ '-1._ L 7 Young produced \\.. 1\ \'5 \& \l.o \'5 \\ \'S IS Total young produced :M o'-\ o'2. ,:,\ Final Adult Mortality '-'--'-\._ \......... L '-\....... L '--X for yd Broods y...... )'C-)<. ')<... >C y -..,L Y-Note: Adult mortality (L = hve, D =dead) CONC: 11.3% Survival and Reproduction Data Replicate number Day_ 1 2 3 4 5 6 7 8 9 10 1 Young produced () () 0 0 0 n 0 0 0 0 Adult mortality L-L L L L L L L '-L 2 Young produced 0 0 D 0 0 0 C) () 0 0 Adult mortality L L \.._ L L L L L l_ I.... 3 Young produced D a D 0 0 0 0 0 a 6 Adult mortality L \..._ \.._ '---L L L L L L 4 Young produced 5 "S 'S lo y Ll L{ '-\ Adult mortality L L L \...._ L L L L L L 5 Young produced \"!. I'-\ \ ""L..... 1:::0 I -::a, , ...... , 13 l!:l Adult mortality \_. L '-L L '-'-'--\..... '--6 Young produced 0 0 0 0 D 0 C) 0 0 0 Adult mortality L '--'-L L L \..._ '--\..__ \.._ 7 Young produced t\ li \'5 l'-\ \\ lb llo 1\o \\o 15 Total young produced 3<c. :*z... 3'2_ 31.:. ¢:, 3:, 3'-\ Final Adult Mortality \._ '--'---'---'--'--'---'-L '---Note: Adult mortality (L = hve, D = dead) Concentration: % Mortality: ()1. Mean Offspring/Female: % Reduction from Control: -\.?... '1 ;> Page 39 of 107 EnvtronmentaiTe:ltln§Sohltlons.lnc. Page 3 of7 Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant-Non-treated Date: _..::;OS_* _l 'l_*cf\ ___ _ CONC: 22.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 a 0 0 0 0 0 0 Adult mortality L \_ L L__ L L L L \...__ l_ 2 Young produced 0 0 0 0 0 0 0 0 C) 0 Adult mortality \.._ \.._ '--L L \...._ \.._ '--L \..__ 3 Young produced C) 0 D 0 0 0 0 0 0 0 Adult mortality L '--\._ L-L '--L L \.... '-4 Young produced cs. 4 l-"-\ '-\ '-\. '5 s. Adult mortality '-'-\,_ \,...._ L. L \.._ \,....__ l_ 5 Young produced \'-\ 'o \b \"\ \\ \'-\ \& Adult mortality '--\..._ '--\_ '-'-L L.. L L.. 6 Young produced D 0 D a a 0 a a 0 () Adult mortality l-'--'-\._ '--'-L '--'-'-7 Young produced IC\ \'5 \.\ lS \8 ,, \'5 \S Total young produced ole. blo 3':> oS '0'-\ Final Adult Mortality L L--'--\._ '-L-'---... \._ '--\...._ Note: Adult mortality (L =live, D =dead) Concentration: % Mortality: (')1. Mean Offspring/Female: ..34.\ % Reduction from Control: - CONC: 45.2% Survival and Reproduction Data Replicate number Day l 2 3 4 5 6 7 8 9 10 1 Young produced L) 0 0 0 0 0 0 0 0 0 Adult mortality '\.__ '-\.__ \..__ \._ L L. L L L 2 Young produced 0 0 0 0 a a 0 0 0 D Adult mortality '-L '--L '--'-"---\.._ L \..__ 3 Young produced D 0 (') (J 0 0 0 0 0 0 Adult mortality \__ '--L '-L L_ L \._ '-\,...._. 4 Young produced c;. '-\ s s 'S 'S "$ '-\ u. Adult mortality \..__ L '-L "-L L \.._ I...... L 5 Young produced IS I"'L IL 11.. I'& 1'-{ II..{ 1'2.. Adult mortality L.. L \_ L L \_ L L. '--\..__ 6 Young produced a 0 0 D 0 0 0 0 0 0 Adult mortality L L L L \..... L L \._ L L. 7 Young produced 1\o l<i' 1'--\ \\ I\ ll.o IS I'S IS lS Total young produced "3\o 3'4 3\ 3'-\ Final Adult Mortality '-'-\_ '--\..__ L '-\..__ \.._ \..__ Note: Adult mortality (L =live, D =dead) Concentration: % Mortality:

01. Mean Offspring/Female: %Reduction from Control: -\.Si. Page 40 of 107

.ETS * * :D ' " .. ) ' l'. {'---' .J Envlnlnm.nt.al Testing Sotutlons. )nc. Page 4 of7 Species: Ceriodaphnia dubia Client: Seguoyab Nuclear Plant-Non-treated Date: o5-\'l-Q'\ CONC: 72.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced () (') a 0 0 D 0 0 0 0 Adult mortality '--L \.._ \..... L.. L L \__ \... \.. 2 Young produced Q 0 0 0 a 0 0 0 0 0 Adult mortality L-'-L L L '---\.._ L_ L 1.... 3 Young produced 0 0 D D 0 0 a a 0 a Adult mortality L '-\.._. '--L-'-L '--\...... '-4 Young produced L., 'S 'S s '-\ '-\ '--\. '-\ Adult mortality \..._ "-\._ "-'-L.. L L L '--5 Young produced \<":::. ,:=, \ "'L ,:;, l'-\ Adult mortality \...... L. '-L_ \._ "--L \._ \...._ '-6 Young produced 0 0 0 0 0 0 0 CJ 6 0 Adult mortality L.. '--'--L \.__ L l._ '-\._ L 7 Young produced \g, \& \\,:, l"-\ \IS \S \'-\ l\ l\o Total young produced o\ '01.. .;6 o'-\ ¢:, Final Adult Mortality \ \...... \.._.. \..._ '--'--\.,._ \..._ '-\._ Note: Adult mortality (L =live, D =dead) Concentration: % Mortality: D't. Mean Offspring/Female: %Reduction from Control: * ?l-37. CONC: 100% Survival and Reproduction Data Replicate number Day I 2 3 4 5 6 7 8 9 10 I Young produced 0 () 0 0 0 0 () 0 0 0 Adult mortality L L-L L L L L-,__ L L 2 Young produced () 0 0 0 0 0 C) 0 0 0 Adult mortality '-\.._ \..._ '-'-L_ L L-L L 3 Young produced () () (_) 0 0 0 0 0_ 0 0 Adult mortality L L '-L L L L '-L L 4 Young produced '$ lo 'S s s "' '-\ Adult mortality L '-\._ L L \._ L \.._ L I.... 5 Young produced \S 1..1. 1"2.. 1'-\ 1'-\ \2_ Adult mortality L L L L L c._ 1...._ L-L_ 6 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality \._.. L L L_ L L L L L L-7 Young produced \'\ \r \\.::. \\ \\o \'-\ \\ \\o \S Total young produced :f\ 3'-\ 3'-\ 3S 3.1.\ 3\ Final Adult Mortality \._ \.,__ \....._. L \.._ \..__ L '--L L-Note: Adult mortality (L =live, D =dead) Concentration: % Mortality:

07. Mean Offspring/Female:

%Reduction from Control: -'-\, Page 41 of I 07 Page 5 of7 Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant-Non-treated Date: OS-1'7..--ot\ CONTROL-2 Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 D () 0 0 0 (j a (\ 0 Adult mortality L-L-L '--L-L L L '--'---2 Young produced 0 0 0 D 0 0 0 0 0 Q Adult mortality L L L '---L L L. L L \.___ 3 Young produced {"') 0 a 0 0 D 0 0 0 a Adult mortality \._ \_. L-L '---\._ L L \._. L 4 Young produced L\ '-'< '-\ 'S '-\ s '-\. '-\ "'\ Adult mortality \_ \.._ '---L L. '--\...___ '--L. '--5 Young produced 1'-l 1'2_ 13 I"Z.. /0 11 .. Adult mortality \..... '-'--'--\.,...._. \,_... '--'-'---'---6 Young produced c a 0 n 0 0 0 0 D 0 Adult mortality L '--'--'--\._ L L L. \..._ L 7 Young produced \'5 ,q \\.o \l.o \\c \Y \I. \"& ,., Total young produced o\ Final Adult Mortality '-'-'-L. L. '--L L L L. Note: Adult mortality (L = live, D = dead) coNe: 100% Intake Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced (.) 0 0 (} (') 0 0 0 0 0 Adult mortality \._. \..._ L-L L L L L L L 2 Young produced 0 0 a 0 () 0 0 0 0 0 Adult mortality L L-L '--L__ L__ L. '--L L. 3 Young produced () 0 0 0 0 0 0 a 0 0 Adult mortality '--L \.__ \.._ L L L L '-\..._ 4 Young produced s "\ I.e. '-\ '-\ $' s s s 1.{ Adult mortality \......._ \_ "----'--l. L '-L \._ L 5 Young produced 1$" 11.. IY 11. 13 1'-1 13 lo 13 Adult mortality L L--L L L L L_ L-L '---6 Young produced 0 0 0 0 0 0 0 0 () 0 Adult mortality L L L L L L L L L L 7 Young produced -z\ \8 1'\ "2.0 \& \& '" \l Total young produced 3'\ :!lS '-II 3la 3S 31 .3\o Final Adult Mortality L '-L \. L L \.. 1..... L. Note: Adult mortality (L =live, D =dead} Concentration: % Mortality: Q't. Mean Offspring/Female: % Reduction from Control: -li.Z1o Page 42 of 107 '"0 CJC< .j;o. w 0 ..... -Q -..J ..J Environmental Te$tlng Solutions, Inc. Control-! Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 4 4 5 5 13 13 13 6 0 0 0 7 16 17 15 Total 33 34 33 11.3% Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 4 5 5 5 12 14 12 6 0 0 0 7 17 17 15 Total 33 36 32 22.6% Day I 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 5 4 6 5 14 13 13 6 0 0 0 7 16 19 15 Total 35 36 34 45.2% Day I 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 5 4 5 5 15 12 12 6 0 0 0 7 16 18 14 Total 36 34 31 TVA I Sequoyah Nuclear Plant, OutfalllOl-Non-treated May 12:.19 2009 Verification of Ceriodaphnia Reproduction Totals 72.6% Replicate number Total 4 5 6 7 8 9 10 Day Replicate number 1 2 3 4 5 6 7 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 {) 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 5 5 5 5 4 4 45 4 6 4 5 5 5 4 4 12 13 II 14 12 12 12 125 5 13 15 15 13 12 13 13 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 18 16 16 15 17 15 15 160 7 18 18 16 14 18 15 14 34 34 32 34 34 31 31 330 Total 37 37 36 32 35 32 31 100% Replicate number Total 4 5 6 7 8 9 10 Day Replicate number 1 2 3 4 5 6 7 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 5 6 4 4 4 4 4 45 4 5 6 4 5 5 5 4 13 13 13 13 14 13 13 130 5 15 14 12 14 13 14 13 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 14 17 16 16 16 16 15 159 7 19 18 16 17 16 14 17 32 36 33 33 34 33 32 334 Total 39 38 32 36 34 33 34 Control-2 Replicate number Total 4 5 6 7 8 9 10 Day Replicate number 1 2 3 4 5 6 7 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 6 4 4 5 4 5 5 48 4 4 4 4 4 5 4 5 14 ll 14 12 13 13 13 130 5 14 12 13 13 13 13 12 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 16 17 15 18 17 15 15 163 7 15 19 16 18 16 16 14 36 32 33 35 34 33 33 341 Total 33 35 33 35 34 33 31 100% Intake Replicate number Total 4 5 6 7 8 9 10 Day RePlicate number l 2 3 4 5 6 7 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 {) 0 {) 0 0 0 0 3 0 0 0 0 0 0 0 5 5 5 5 5 4 4 47 4 5 4 6 4 4 5 5 13 13 12 13 14 14 12 130 5 15 12 14 14 12 13 14 0 0 0 {) 0 0 0 0 6 0 0 0 0 0 0 0 17 17 16 15 15 15 15 158 7 19 19 21 18 19 20 18 35 35 33 33 34 33 31 335 Total 39 35 41 36 35 38 37 8 9 10 Total 0 0 0 0 0 0 0 0 0 0 0 0 4 4 4 45 13 14 13 134 0 0 {) 0 17 16 16 162 34 34 33 341 8 9 10 Total 0 0 0 0 0 0 0 0 0 0 0 0 6 6 4 50 13 13 12 133 0 0 0 0 16 15 15 163 35 34 31 346 8 9 10 Total 0 0 0 0 0 0 0 0 0 0 0 0 4 4 4 42 lO 14 12 126 0 0 0 0 17 15 17 163 31 33 33 331 8 9 10 Total i 0 0 0 0

  • 0 0 0 0 I 0 0 0 0 i 5 5 4 47 I 13 13 13 133 ] 0 0 0 0 i 18 19 17 188 36 37 34 368 J

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(%) 1 2 3 Control-1 33 34 33 11.3% 33 36 32 22.6% 35 36 34 45.2% 36 34 31 72.6% 37 37 36 100% 39 38 32 Control-2 33 35 33 100% Intake 39 35 41 OutfalllOl: Dunnett's MSD value: 1.831 PMSD: 5.5 Dunnett's MSD value: 1.374 PMSD: 4.2 TV A I Sequoyah Nuclear Plant, OutfalllOl Non-treated May 12-19, 2009 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses 4 34 32 36 35 32 36 35 36 MSD= PMSD= Project number; 5444 -----** --.... ./. ,-(\/, /I Replicate number Survival Average reproduction Coefflcien( of Percent reduction from 5 6 7 8 9 10 (o/o) (offspring/female) variation (%) pooled controls ('Yo) 34 32 34 34 31 31 100 33.0 3.8 Not applicable 36 33 33 34 33 32 100 33.4 4.5 -1.2 32 33 35 34 33 33 100 34.1 4.0 -3.3 35 33 33 34 33 31 100 33.5 4.9 -1.5 35 32 31 34 34 33 100 34.1 6.3 -3.3 34 33 34 35 34 31 100 34.6 7.2 -4.8 34 33 31 31 33 33 100 33.1 4.1 Not applicable 35 38 37 36 37 34 100 36.8 5.7 -11.2 -----Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test Lower PMSD bound determined by USEPA (lOth percentile) = 13%. Upper PMSD bound determined by USEP A (90th percentile) = 4 7%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200la; USEPA, 200Ib). USEP A 2000. Understanding and Accounting for Method Variability in Whole Effiuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEP A 200 I a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2-Appendix. EPA-82l*B-O 1-004 and EPA-821-B-0 1-005. US Environmental Protection Agency, Cincinnati, OH. (lQ (t) (JJ 0 ...., ...... Q --.1 TV A I Sequoyah Nuclear Plant, OutfalllOl Non-treated May 12-19, 2009 Statistical Analyses

  • ) Environmental Testing Solutions, Inc. Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/12/2009 End Date: 5/19/2009 Sample Date: Conunents:

Cone-% Control-! Control-2 11.3 22.6 45.2 72.6 100 Intake Cone-% Control-I Control-2 11.3 22.6 45.2 72.6 100 Intake 33.000 33.000 33.000 35.000 36.000 37.000 39.000 39.000 Mean 33.000 33.100 33.400 34.100 33.500 34.100 34.600 36.800 Test ID: Lab ID: Protocol: 2 3 34.000 33.000 35.000 33000 36.000 32.000 36.000 34.000 34.000 31.000 37.000 36.000 38.000 32.000 35.000 41.000 N-Mean Mean 0.9970 33.000 1.0000 33.100 1.0091 33.400 1.0302 34.100 1.0121 33.500 1.0302 34.100 1.0453 34.600 1.1118 36.800 CdFRCR Sample ID: ETS-Envir. Testing Sol. Sample Type: Test Species: 4 5 6 7 34.000 34.000 32.000 34.000 35.000 34.000 33.000 31.000 32.000 36.000 33.000 33.000 36.000 32.000 33.000 35.000 35.000 35.000 33.000 33.000 32.000 35.000 32.000 31.000 36.000 34.000 33.000 34.000 36.000 35.000 38.000 37.000 Transform: Untransformed Min Max CV% N 31.000 34.000 3.779 10 31.000 35.000 4.140 lO 32.000 36.000 4.508 10 32.000 36.000 4.019 10 31.000 36.000 4.925 10 31.000 37.000 6.252 10 31.000 39.000 7.235 10 34.000 41.000 5.700 10 Auxiliary Tests Statistic Kolmogorov D Test indicates normal distribution (p > 0.01) 0.653808415 Bartlett's Test indicates equal variances (p = 0.26) 6.457315922 The control means are not significantly different (p = 0.87) 0.170664037 8 34.000 31.000 34.000 34.000 34.000 34.000 35.000 36.000 t-Stat -0.500 -1.374 -0.624 -1.374 -1.998 Hypothesis Test (!-tail, 0.05) NOEC WEC ChV TU MSDu MSDp Dunnett's Test 100 >100 I 1.83092058 0.055482442 Treatments vs Control-! Point % IC05 >100 JCIO >100 ICI5 >100 IC20 >100 IC2S >100 IC40 >100 1C50 >100 SD 95%CL Linear Interpolation (200 Resamples) Skew TV A I Sequoyah Nuclear Plant, Outfall I 01 DMR-Discharge Monitoring Report CD-Ceriodaphnia dubia 9 10 31.000 31.000 33.000 33.000 33.000 32.000 33.000 33.000 33.000 31.000 34.000 33.000 34.000 31.000 37.000 34.000 !-Tailed Isotonic Critical MSD Mean N-Mean 33.783 1.0000 2.287 1.831 33.783 1.0000 2.287 1.831 33.783 1.0000 2.287 1.831 33.783 1.0000 2.287 1.831 33.783 1.0000 2.287 1.831 33.783 1.0000 Critical Skew Kurt 1.035 0.254140335 -0.2360760 I 15.08627224 2.100922029 MSB MSE F-Prob df 3.416666667 3.205555556 0.389506936 5, 54 sqnl 0 1_ 05-12-09data "'C (1q .... 0\ 0 ..., .I TVA I Sequoyah Nuclear Plant, OutfalllOl-Intake Non-treated

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'.{ *, .'! ': ' J .. Environmental Testing Solutions, Inc. Start Date: 5112/2009 Test ID: End Date: 5/19/2009 Lab ID: Sample Date: Protocol: Comments: Cone-% I 2 3 Control-! 33.000 34.000 33.000 Control-2 33.000 35.000 33.000 11.3 33.000 36.000 32.000 22.6 35.000 36.000 34.000 45.2 36.000 34.000 31.000 72.6 37.000 37.000 36.000 100 39.000 38.000 32.000 Intake 39.000 35.000 41.000 Cone-% Mean N-Mean Mean Control-I 33.000 0.9970 33.000 Control-2 33.100 1.0000 33.100 11.3 33.400 1.0091 33.400 22.6 34.100 1.0302 34.100 45.2 33.500 1.0121 33.500 72.6 34.100 1.0302 34.100 100 34.600 1.0453 34.600 Intake 36.800 1.1118 36.800 Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.01) F-Test indicates equal variances (p = 0.22) The control means are not significantly different (p = 0.87) Hypothesis Test (!-tail, 0.05) Homoscedastic t Test indicates no significant differences Treatments vs Control-2 May 12-19, 2009 Statistical Analyses Ceriodaplmia Survival and Reproduction Test-Reproduction CdFRCR Sample ID: ETS-Envir. Testing Sol. Sample Type: FWCHR-EPA-821-R-02-013 Test Species: 4 5 6 34.000 34.000 32.000 35.000 34.000 33.000 32.000 36.000 33.000 36.000 32.000 33.000 35.000 35.000 33.000 32.000 35.000 32.000 36.000 34.000 33.000 36.000 35.000 38.000 Transfonn: Untransformed Min Max CV% 31.000 34.000 3.779 31.000 35.000 4.140 32.000 36.000 4.508 32.000 36.000 4.019 31.000 36.000 4.925 31.000 37.000 6.252 31.000 39.000 7.235 34.000 41.000 5.700 7 34.000 31.000 33.000 35.000 33.000 31.000 34.000 37.000 N 10 10 10 10 10 10 10 10 Statistic 0.955419481 2.3431952 0.170664037 MSDu 8 34.000 31.000 34.000 34.000 34.000 34.000 35.000 36.000 t-Stat -4.670 MSDp 1.373940728 0.041508783 TVA I Sequoyah Nuclear Plant, Outfalll01 -Intake DMR-Discharge Monitoring Report CD-Ceriodaplmia dubia 9 10 31.000 31.000 33.000 33.000 33.000 32.000 33.000 33.000 33.000 31.000 34.000 33.000 34.000 31.000 37.000 34.000 1-Tailed Critical MSD 1.734 1.374 Critical Skew Kurt 0.868 0.544660572 0.427540628 6.541089535 2.100922029 MSB MSE F-Prob df 68.45 3.138888889 1.9E-04 1, 18 sqnl01_05-12-09data ""= IJQ (!) .... -...1 0 ,j Environmental Testing Solutions, Inc. Concentration Control 11.3% 22.6% 45.2% 72.6% 100% 100% Intake TV A I Sequoyah Nuclear Plant, OutfalllOl -Non-treated May 12-19, 2009 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Project number: 5444 Reviewed by: G vf.Aho.-7

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Inc. Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant-Non-treated Concen-Parameter tration 11.3% 22.6% 45.2% 72.6% 100% 100% Intake Page 49 of 107 Page 7 of7 Date: OS* \'"1-cf\ !ETS ) Tutln.g SaluUcna.lnc. Page 1 of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: TVA Facility: Sequoyah Nuclear Plant NPDES #: TN 0026450 Project#: Dilution preparation information: Dilution prep (%) 11.3 22.6 Effluent volume (mL) 282.5 565 Diluent volume (mL) 2217.5 1935 Total volume (mL) 2500 2500 Test organism information: 45.2 72.6 1130 1815 1370 685 2500 2500 Organism age: l.o. 1"\.ou.t c:. 0 Date and times organisms were born between: 100 2500 0 2500 Organism source: '&A\'C..H Vp O'S*\\-0'\ Transfer bowl information: pH= SU Temperature= oc "1. '-\ .l.,. Average transfer volume: Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding 0 2 3 4 5 6 7 Page 50 of 1 07 County: Hamilton Treatment: UV -treated Outfall: 101 Comments: Each concentration was treated for 2 minutes with a UV sterilizer to remove pathogenic interferences. Test information: Randomizing template: Incubator number and 2>e, shelf location: Artemia CHM number: t.\-\ I-\ t.\ 1.\ '-\ Date I Time in: OS*!P.*o"\ \'2.00 Date I Time out: oS*z.o*d"' l't'l.O Total drying time: "Z.'(

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Page 2 of6 EnvlronmentlfTtstln; Solutkms.lrl,, Species: Pimephales promelas Date: OS*n-o'\ Client: TV A I Sequoyah Nuclear PlaJ}t-UV-treated Survival and Growth Data Day CUNTROL 11.3% 22.6% A B c D E F G H I J K L 0 JO /0 10 IC lO 10 /0 10 10 10 /0 10 1 /0 10 I{J IO /D 10 IO IO 10 IO 10 IO 2 (0 to 10 /0 10 10 10 10 10 to ID IO 3 /D ID 10 to 10 /() /() It) tD to IO IO 4 lO to '( 0 IO JD 10 to /0 10 10 10 ID 5 IO lo to 10 10 10 10 to 10 tO 10 ID 6 to 10 tO ID ID /0 10 /C ID 10 10 IO 7 /D [0 ID (() fD tO (0 JO 10 10 1,t:A.. A= Pan weigh_t (mg) Tray color \,k bt*v-. )3,g£" l3.bd. /3. 8i> Y.6o Analyst: J'J,..bO Ill. I If /3.g6 )It IS' 14.14 14. Date:_ ld--0'\ B = Pan + Larvae weight (mg) ""'t... r

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.;l;).3S d.'l J.D.lb Analyst: Date: o 5 C = Larvae weight (mg) =B-A l...oi 11.>,'\S c,.s'\ 1.15 8.S2. 11-5\o ,.,., 1.0I.o Weight per initial number ",_, h'l rV " 1\ \D ,_,'? oflarvae (mg) " r!.f' ""' ,....-v \0 = C I Initial number of larvae .., " '-41 ,... 'b " I:>' 'I) I;)' C)' \)' I;)' o* o* o* io* o* Average Percent weight per reduction 0.1 .. 1 s.'i1. 0. iOb initial from control number of (%) larvae (mg) Comment codes: c == clear, d == dead, fg = fungus, k == killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 51 of 107

Page 3 of6 ) Environmentlllt:stln.g Soludor.s. tnt.. Species: Pimephales promelas Date: Client: TVA I Sequoyah Nuclear Plant-UV-treated Survival and Growth Data Day 45.2% 72.6% 100% M N 0 p Q R s T u v w X 0 (0 l() 'D ID /0 lb 10 lfJ 10 10 10 10 1 10 fO /0 10 tD 10 10 ID 10 /0 10 10 2 IO /0 JD /Q 10 10 lb /Q IO /0 /() 10 3 10 ID /0 ID IO IO IO /() 10 IQ 10 /0 4 IO 10 /0 10 10 10 10 10 10 /0 10 10 5 to 10 10 lO /0 /0 10 10 10 If) tO IO 6 to tO IO 10 10 /D It> /'V l\) ro /0 /Q 7 /CJ 10 IC 10 10 10 10 10 /0 tD ID 10 A= Pan weight (mg) Tray color code:: S"tf 13.4f /3. 6& 14. 35' 14.41 13.31{ 13. f>O N.lq Analyst: Date: 6 .c--1).-ot.\ 8 = Pan + Larvae weight (mg) 1'\L..r" ;J."J.S). Analyst: J.l.ll jq,qa.

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1-S' 1:*\S Weight per initial number .:r'V <h c., 7 ' ,lf of larvae (mg) ff\"') "V"' q,.'? .>.. = C /Initial number of larvae " " \1) "" .:J' 1\ (f !;)' o* o* o* o* jo* o* 0' Average Percent weight per reduction Q,,,"\ -l.\ .'-l "T. -2..\7,. initial from control -o.l"7 0. "'" & .. number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 52 of 107 Page 4 of6 Species: Pimephales promelas Date: Client: TVA I Sequoyah Nuclear Plant-UV -treated Survival and Growth Data Day 100% Intake y z AA BB 0 10 /0 /() IO 1 /0 ID 10 10 2 ID /0 10 .,-... 3 to ( ;,0 4 /D /D 10 10 5 In IO 10 /0 6 /Q 10 tO 10 7 /0 10 LO tO A= Pan weight (mg) Tray color code:: l4.*u l},qb Analyst: ,.,-l..r Date: B =Pan + Larvae weight 'J.D. 8;). (mg) ;).1.15 J-1.11-lll.38 Analyst: f"'Lf"" Date: ()':,--OC\ C = Larvae weight (mg) =B-A fo.lo 1.. eo.'-\1.-Weight per initial number "'> of larvae (mg) o..c., = C I Initial number of larvae \,p -.!' -.. 'D t>' \;)' Average Percent weight per reduction 5.17 .. initial from control o.taS'\ number of (%) larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: _&.__ Comments: Page 53 of 1 07 !'t> (JI """ 0 -. -0 .. *

  • Environmental Testing Solutions, Inc Cu.(Utnttioa

(%) Replic.&e A Control B c D E F 11.3% G H I 22.6'/o J K L M 45.2% N 0 p Q 72.6°/* R s T u 100% v w X y 100% intake z AA BB Outfall101: DunoeU's MSD vaJue: PMSD: Intake: Dunuett's MSD value: PMSD: IaJtW **mber of 1:1111"\'lle 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 0.0710 10.5 0.0520 7.6 Fiu.l aumber uf lalvae 10 10 10 10 10 10 10 10 10 10 10 9 10 10 10 10 10 10 10 10 10 10 JO 10 10 10 10. 10 A= Paa weie;hl (m&) 13 85 13.62 13.80 14.45 14 60 13 77 12.60 14 14 13.86 14 15 14.14 14.31 15 59 13.95 13.09 13.45 13.68 14.35 14 41 13.34 13.60 14 40 14 19 13 44 14.20 14 82 14 20 12.96 MSD= TVA I Sequoyah Nuclear Plant, OutfalllOl UV-treated May 12-19,2009 Pimepha/es promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Not for Compliance Assessment Internal Laboratory QC B ""' Paa + Larvae ,.;gbl (m&) WciP,t I Survivia£ Meao "<lpll of varia1hNI [aitial IUIPbu weigbl (mg) =A-8 umber-of larvae (me.) Sarvivlag .. mbcr of (M.alt*el&l'f>'t'MU"l'l*la' of ld.I"VK (mg) larne(m&) .... w .rw ... (%) 20.22 6.37 0.637 0.637 2105 7.43 0.743 0.684 6.8 0.743 20.75 6.95 0 695 0.695 21.04 6.59 0 659 0.659 22.35 1.75 0 175 0.775 22.29 8.52 0.852 0.789 5.4 0.852 20 16 7 56 0 756 0.756 21 86 7.72 0.772 0.772 21 23 7.37 0 737 0.737 21 42 7.27 0.727 0.724 1 8 0 727 21.20 7.06 0.706 0.706 20 84 6.53 0.726 0.653 22.52 6.93 0.693 0 693 21.17 7.22 0 722 0.714 4.6 0.722 19.92 6.83 0.683 0.683 21 01 7.56 0.756 0.156 20.50 6.82 0.682 0.682 21.03 6.68 0.668 0.684 3.8 0.668 21.06 6.65 0.665 0.665 20.56 7.22 0.722 0.722 19.91 6.31 0.631 0.631 21.04 6.64 0.664 0.698 8.5 0.664 21.70 7.51 0.751 0.751 20.89 7.45 0.745 0.745 21.15 6.95 0.695 0 695 21 17 6.35 0.635 0.659 41 0.635 20 82 6.62 0 662 0.662 19 38 6 42 Q642 .. -0.642 Mmimum Signdi.cant Dtfference Percent Minimum Sig,nHlcant Difference Project aumber* 5444 Keveiwed by: /"()/LAAt- \. ./I Muasurvh*af Meu weiehtt Codlicieatol Peruut reduc:tioa frota Jailial umber of varladoa tid** w.1p1 toafroJ lalvae (mg) pOI-Jtl.t DUlllbu o(\arvM) % 100.0 0.684 6.8 Not applkable 100.0 0.789 5.4 -15.4 97.5 0.706 5.3 -3.3 100.0 0.714 4.6 *4,4 100.0 0.684 3.8 -0.1 100.0 0.698 8.5 -2.1 100.0 0.659 4.1 3,7 PMSD is a measure of test prectston. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test Lower PMSD bound determined by USEPA (LOth percentile) 12% Upper PMSD bound determined by USEPA (90th 30%. Lower and upper PMSD bounds were deterrruned from the I Oth and 90th percentile, respectively, ofPMSD data from EPA's WET lnlerlaboratory VOJiabilily S!udy (USEPA, 200 l a; US EPA, 200 I b) USEPA 200la. 200lb. Fma1 Report Interlaboratory Variabthty Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-Ql-Q04 and EPA-821-B-Q1-Q05. US Environmenla1 Protection Agency, Cincinnati, OH ""= (JQ til til 0 ...... ...... 0 -...l TVA I Sequoyah Nuclear Plant, Outfall101 UV-treated S ... .. **.* May 12-19,2009 Statistical Analyses j Environmental Testing Solutions,. Inc. Larval Fish Growth and Survival Test-7 Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: End Date: 5119/2009 Lab!D: ETS-Envir. Testing Sol. Sample Type: Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: Comments: Cone-% 1 2 3 4 D-Control 0.6370 0.7430 0.6950 0.6590 11.3 0.7750 0.8520 0.7560 0.7720 22.6 0.7370 0.7270 0.7060 0.6530 45.2 0.6930 0 7220 0.6830 0.7560 72.6 0.6820 0.6680 0.6650 0.7220 100 0.6310 0.6640 0.7510 0.7450 Intake 0.6950 0.6350 0.6620 0.6420 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat D-Control 0.6835 1.0000 0.6835 0.6370 0.7430 6.776 4 11.3 0.7888 1.1540 0.7888 0.7560 0.8520 5.450 4 -3.522 22.6 0.7058 1.0326 0.7058 0.6530 0.7370 5.308 4 -0.745 45.2 0.7135 1.0439 0.7135 0.6830 0.7560 4.598 4 -1.004 72.6 06843 1.0011 0.6843 0.6650 0.7220 3.834 4 -0.025 100 0.6978 1.0208 0.6978 0.6310 0.7510 8.544 4 -0.477 Intake 0.6585 0.9634 0.6585 0.6350 0.6950 4.083 4 Auxiliary Tests Statistic Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.957272053 Bartlett's Test indicates equal variances (p = 0.83) 2.111745119 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp Dunnett's Test 100 > 100 1 0.072014927 0.105361999 Treatments vs D-Contro1 Point !COS JC10 IC15 IC20 JC25 IC40 IC50 % 60.362 >100 >100 >100 >100 >100 >100 SD 95% CL(Exp) Linear Interpolation (200 Resamples) Skew TVA I Sequoyah Nuclear Plant, Outfall IOJ DMR-Discharge Monitoring Report PP-Pimephales promelas !-Tailed Isotonic Critical MSD Mean N-Mean 0.7361 1.0000 2.410 0.0720 0.7361 1.0000 2.410 0.0720 0.7096 0.9640 2.410 0.0720 0.7096 0.9640 2.410 0.0720 0.6910 0.9387 2.410 0.0720 0.6910 0.9387 Critical Skew Kurt 0.884 0.185406404 -0.99302071 15.08627224 MSB MSE F-Prob df 0.0061735 0.001785833 0.023020582 5, 18 sqnl01_05-J 2-09data-uv '"C ;:.l (JQ VI 0\ 0 ...., ...... 0 -..J TV A I Sequoyah Nuclear Plant, OutfalllOl -Intake UV-treated May 12-19, 2009 Statistical Analyses Environmental Testing Solutions, Inc. Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 End Date: 5/19/2009 Sample Date: Comments: Cone-% 1 2 D-Control 0.6370 0.7430 11.3 0.7750 0.8520 22.6 0.7370 0.7270 45.2 0.6930 0.7220 72.6 0.6820 0.6680 100 0.6310 0.6640 Intake 0.6950 0.6350 Cone-% Mean N-Mean D-Control 0.6835 1.0000 11.3 0.7888 1.1540 22.6 0.7058 1.0326 45.2 0.7135 1.0439 72.6 0.6843 1.0011 100 0.6978 1.0208 Intake 0.6585 0.9634 Test ID: Lab ID: Protocol: 3 0.6950 0.7560 0.7060 0.6830 0.6650 0.7510 0.6620 Mean 0.6835 0.7888 0.7058 0.7135 0.6843 0.6978 0.6585 PpFRCR ETS-Envir. Testing Sol. FWCHR-EP A-821-R-02-0 13 4 0.6590 0.7720 0.6530 0.7560 0.7220 0.7450 0.6420 Transform: Untransformed Min Max CV% o.6370 o:7430 6.776 0.7560 0.8520 5.450 0.6530 0.7370 5.308 0.6830 0.7560 4.598 0.6650 0.7220 3.834 0.6310 0.7510 8.544 0.6350 0.6950 4.083 Sample 10: Sample Type: Test Species: N 4 4 4 4 4 4 4 Auxiliary Tests Statistic Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95557791 F-Test indicates equal variances (p = 0.40) 2.96680498 t-Stat 0.934 Hypothesis Test(l_-tail,O.@ -** MSDu MSDp Homoscedastic t Test indicates no significant differences 0.05203226 0.0761262 Treatments vs D-Contro1 TV A I Sequoyah Nuclear Plant, Outfall 101 -Intake DMR-Discharge Monitoring Report PP-Pimephales promelas !-Tailed Critical 1.943 Critical 0.749 47.46722794 MSB 0.00125 MSD 0.0520 MSE 0.001434 Skew Kurt 0.566287708 -0.42008898 F-Prob df 0.386520296 1,6 sqnlOJ '"e "" (10 rt> IJl -...1 0 ,.....

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T<.o*' -----Environmental Testing Solutions, Inc. TVA I Sequoyah Nuclear Plant, Outfall lOt-UV-treated May 12-19,2009 Pimephales prome/as Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number: 5444 ----------------- Reviewed Page 5 of6 Species: Pimephales promelas Date: OS* \"!.* 0'\ Client: TV A I Sequoyah Nuclear Plant-UV-treated 11.3% 22.6% 45.2% 72.6% 100% 100% Intake Page 58 of I 07 .ETS * <It 1:1 * ** : ) '" I: {'--' :. ) EnvlfonmentalTatlng.Solvtltns.lnc. 11.3% 45.2% 72.6% 100% 100% Intake Species: Pimephales promelas Client: TVA I Sequoyah Nuclear Plant-UV-treated Parameter Page 59 of 1 07 Page 6 of6 I I I I I I I .., I I =-d I d -t Environmental Testing Solutions, Inc. Analyst I f'l\l.A'" Date analyzed (; 5 J J_ .. 0'\ Calibration: L b I d a oratory contro standar : Reference standard number lnSS Total Residual Chlorine Page d-. 'i Page _I_ of_l_ (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Meter: Accmnet Model AR25 pH/Ion Meter True value (TV) (mg!L) 0.50 Sample characteristics Iodide reagent: 111\a l '1-Acid reagent: _ l:llR. 33o to bracket the chlorine levels of the samples. Measured value (MV) (mg!L) 0, S'Oi> s LO.ooo D LO i-ll. % RS = MV I TV x 100 (acceptable range= 90 to 110%) """" J\1"1 /(>J.J.'?t>

  • -Sample ID Sample characteristics Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. L b d a oratory control stan ard: Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 number (mg!L) (mg/L) (acceptable range= 90 to 110%) '!nss t+-3 . --o:-so D;tfttl

'age 60 of I 07 Reviewed by Date reviewed _-, I I I I I I II --'I.....: *Er * * * * ) "*'----I !> Environmental Testing Sofutians,lnc. Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pWlon Meter Page _5 I Page _I_ of_l __ Analyst I ('1\.Lf" Date analyzed 0 S'"-j L.j -o1 Iodide reagent: ll"A (2 3' 3 J Acid reagent: _ :ri\A. 3 3 () b I La oratory contro standard: Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 number (mg/L) (mg/L) (acceptable range= 90 to 110%) .l.t\ ,,] 0.50 o.li6lf q J.. Sample characteristics Sample ID Sample characteristics Note: All samples were analyzed in excess of EPA recommended holding time (IS minutes) unless otherwise notecl a oratory contro stan ar : L b I d d Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 number (mg/L) (mg/L) (acceptable range= 90 to 110%) .J.,..IUJ Ptj -----o.so----: ()* l.f6q ""

'age 61 of 107 Rev1ewed by Date reviewed 11 r r 1: I I I I

  • * '3 "'-*** ic / c/' _) Environmental Testing Solutions, Inc, Analyst I L\40 Date analyzed 09 Lb I a oratory contro standard:

Reference standard number Total Residual Chlorine Page_32__ (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0. l 0 mg!L Meter: Accumet Model AR25 pfL'Ion Meter True value (TV) (mg/L) 0.50 Sample characteristics Measured value (MV) (mg/L) 0.'50, s '-o .oan.1.'\ D LO. 00011.3 Iodide reagent: I ""0\.) 1233 7 Acid reagent: ::I:Nr23X? % RS = MV I TV x 100 (acceptable range= 90 to 110%) I Sample ID Sample characteristics I I I I I L b t I a ora ory contro stan d d ar : Reference standard I number -r:_ ' ,-. Lr-_

  • I age 62 of 1 07 True value (TV) Measured value (MV) (mg/l.) (mg!L) 0.50 0.4lb6 L().DOISlP L 0 .000\\.DCO L. o. o ao llo"1 L.O. coo003B'f LO.OI4l1 L. o. OOS7 I L 0 .co I % RS = MV I TV x 100 (acceptable range= 90 to 110%) q Z-0 /. Reviewed by Date reviewed .__o_5_-_l'}-.:._;-u=-*

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= 1.0 mg CaCO)L Titrant norma 'tty and multiplier determination: pH of Normality Deionized Titrant check Begin End. water reference standard ml ml =4.5 s.u. number number t.t,5 Tt-li2-62..CJ t).O 1/."1-Lb I d d a oratory contro stan ar : Reference standard True value Sample number (TV) volume Begin (mg CaC0 3/L) (ml) ml SNSS 100 100 11.--::r-M.' Jk atnx spJ e recovery: Reference standard Spike value Sample number (SV) volume Begin (me CaCO:YL) (ml)_ ml INS.SvS4 so laO Sample alkalinity (B) (mg CaCOJIL) lo3 s l amp1e measurements: Titrate samples to pH= 4.50 S.U. Time initiated I \ 1 5Ll* Time completed

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Normality (N) of H 2 S04 pH Factor or Multiplier Total = (5 ml Na 2 C0 3 x 0.05)/E = (Nx 50000)1 100 ml sample ml =0.25/E =Nx500 (E) (acceptable range= 0.0180-0.0220) 11.1 0.0-Rl-Y. End Total Multiplier ml ml 'eq 10.1-Multiplier End Total Multiplier ml ml o-t-o 10,"1-ID.1-Measured spike value (MV) MV=A-B (mg CaCO:YL) 4-::t-10.":1-Alkalinity (MV) % RS = MV I TV x 100 (mg CaCOJIL) (acceptable range = 90 to 110%) cvs qs"i.c Alkalinity (mg CaCO:YL) o/oRPD= {(S-D) /((S+D)/2)} x 100 (acceptable range=+/- 10%) s D Spike alkalinity (A) (mg CaCOJIL) l\0 Ofo.R= MV I SV x 100 (acceptable range = 75 to 125%) Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOJIL) OS-lYJ.ty::, B rn 1JtiJ 1')\) 31-.tl 5.1-l () .t-vl fx5. () t)-0C1 (!; J 4J..1-lti_\?'t \ lol b:s-6-e.oCJ 6Sv.J 'D.O 2_,q ir.t'ii'7. [):). D Y-0'1 J .,') .'1 {u. {) 2,,\ 05-t>:>*Ol1 A fnlf.S l-};.{) (v.D JOI,D (..,.() Lo4* o5.o5*oc.,P. lliQ.\) R::t-f:S.-r-lol

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  • 3 it Alkalinity B) Page 33 Page of lo Analyst l :c I Matrix: Water, RL = 1.0 mg CaCOy'L Date Titrant normality and multiplier determination:

pn 01 N"P"1!0ijty Deionized Titrant check JSegm .. water reference standard ml ml -4.5 s.u. number number L b a oratory control standard: Reference standard True value Sample number (TV) volume Begin (mg CaCO:YL) {ml) ml :r t-)SS fo 54-100 100 a-t.o M,. 'k atrvc sp1 e recovery: Reference standard Spike value Sample number (SV) volume Begin {mg CaCO:YL) (ml) ml ]:NSSt..u5Y* 5o 100 tO.-=t-Sample alkalinity (B) (mg CaC0 3/L) 110 s l ample measurements: Titrate samples to pH= 4.50 S.U. Time initiated , ............... Time completed Normality (N) of H 2 S04 pH Factor or Multiplier ...Tntll) ml (E) End ml .lf3:t End ml :l,t.?.D (5 ml Na 1 C0 3 x 0.05)/E (Nx 50000)/ 100 ml sample l\ 'l.<;/F. Nx500 (acceptable range 0.0180 -O.u-.-.u, I . Total Multiplier ml CiLJ-10.1-Multiplier Total Multiplier ml 15-3 I D.-:-1-Alkalinity (MV) (mg CaCO:Yl) Alkalinity (mg CaCO.YL) s D ..,...... % RS= MV /TVx 100 (acceptable range = 90 to 110%) .i'n %RPD= ((S-D) /[(S+D)/2]} x 100 (acceptable range= ::1: 10%) Spike alkalinity (A) (mgCaC(}y'L) !(.QO Measured spike value (MV) % R = MV I SV x 100 MV=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) ='() Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier {mg CaCOJIL) OG-DC\ *0'1 Inn ;/l.>.C 35.5 q.s \i);-:f-roo os*o5. ocw \ ?AS 41;.9 IO.'+ HO D\o !+tull ) D.O ':f-. u i-.v £1 I 2-r:f.L..> IJ.f*1 '1,\ 'Ito

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1,:::,3. 5tp os<ls. o-z_ CDw .-.fr! ( 26" 3<o 16 !'!til <-----.4-\--e£ t... I tpO 6 0i 4 of 107 I $C - ' ':f:.,!-; I Date reviewed: f 05dJ): ... Reviewed by: ; --.: ' -- Page ---T" } Environmental Testing Page 3 of_lo=--Analyst t-l-""6'-=St.:""-:;.._--:----IJ Date analyzed [ 05 -I 0 -O.:J' l Alkalinity (SM.-2320 B) Matrix: Water,-RL = 1.0 mg CaC03/L Titrant normality and multiplier determination: pH of Deionized Titrant check Begin Enu water reference standard ml ml 4.5S.U. number number L b I d a oratory contro standar : Reference standard True value Sample number (TV) volume Begin (mg CaCOJIL) (ml) ml J: N ss lo5'-l-100 100 D.D .k atru: spz e recovery: Reference standard Spike value Sample number (SY) volume Begin (mg CaCOJIL) (ml) ..mJ.,..... . . .... Sample alkalinity (B) (mg CaCOJIL) 110 measurements: Titrate samples to pH= 4.50 S.U. Time initiated I "'-- .... Time completed ..__ ______ -* J.' ml (E) End ml C):'f End ml .'1 Normality (N) ofH 1 S0 4 pH Factor or Multiplier 'f5 ml Na 1 C0 3 x 0.05)/E -(N x 50000)/100 ml sample 0.1;::1/J!. (acceptable range 0.0180 -0.0220) Nu:_ 500 -1'-Alkalinity (MV) % RS :MY /TYx 100 Total Multiplier (mg CaC0 3/L) (acceptable range ml =90 to 110%) <14 io:1-ID\ 1010lo Alkalinity %RPD= Total Multiplier (mg CaCOJIL) {(S-D) /((S+D)/21} x 100 (acceptable range=+/- 10%) 1 D-:t-\ lO Spike alkalinity (A) Total Multiplier (mg CaCOJIL) ml lb.i-Measured spike value (MY) % R: MY I SY x 100 MY=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) O?oD l'uou1-o Date reviewed: I 05 _ \ o-09 J

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-Alkalinity B) Page Lf Page -of to -Analyst I Matrix: = 1.0 mg CaC03/L Time initiated I Date analyzed t-c ..... r: I' ztrant norma 'tty an d multiplier determination: pl!_of Normality Deionized .-h .. .-k Begin End water reference standard mr-o.l 4.5 s.u. number number L b t 1 d d a ora ory contro stan ar : Reference standard True value Sample number (TV) volume Begin (mg CaCOJ/'L) (ml) ml 100 100 11-f-.?...- u* 'k atru: spt e recovery: Reference standard Spike value Sample number (SV) volume Begin (mg CaC03/L) (ml) ml r"Nss <.co4-.Qa'D c1S Sample alkalinity (B) (mg CaCOJIL) \CO S I amp* e measurements: Titrate samples to pH= 4.50 S.U. Time completed 1---.:::::::..----jb=db---l Total ml (E) End ml End ml 32.'73 Normality (N) of HlS0 4 pH Factor or Multiplier = (5 ml Na 1 C0 3 x 0.05)/E = (N x 50000)/100 ml sample lacce Total ml q_3 Total ml 1-D L4) 0.251E Nx500 .. = n R180 -0.0220) 1 ..... Alkalinity (MV) % RS = MV I TV x 100 Multiplier (mg CaCO:v'L) (acceptable range =90 to 110%) ) D.-:} 100 Alkalinity %RPD= Multiplier (mg CaCO:v'L) {(S-D) /[(S+D)/2]} x 100 (acceptable range=+/- 10%) Spike alkalinity (A) Multiplier (mg CaCO:v'L) IIJ.t-3DD Measured spike value (MV) % R = MV I SV x 100 MV=A-B (acceptable range (mf CaCOJIL) = 75 to 125%) <X co Sample volume Begin End Total Alkalinity Sample number SampleiD (ml) ml ml ml Multiplier (mg CaC0 3/L) -rMBtF 3 as 35-3 L4) \D.-1--'\ l) \l)q -( JA-BFf2-l!Tf \ l.h\":J-.9 14 ' 110 \ ll '2-?,":?.9 ,Yv.'S 'Q-I.P l I 0 -:. IH ' .3 .1-\()_1') l-13. \ l.-4 \10 \1 \ oso'-l

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u)' I &J .Cl_l], 0"' 0":>0'-.1.,(a I %.0 J-jC{ .l 3.1 (;i [o\J " 3 +----3:o (o4--asoi.IO D-0 ,:ic-Q\ 0 () s 0"\. 01.\ 1J'A-lt \Pi.) HII1Al j h_"'.:}-6!.1-(.2 '53 0" "2:1 ..! z... .j S!+ << .-=t (_;t: 50 lage 66 of 1 07 Reviewed by: Date reviewed: I 05-IO.OCJ I I I I I I I I I I I I I 1--Alkalinity B) Page 3\p Page 0 Analyst I Matrix: Water;-R.L = 1.0 mg CaCOJL Date analyzed *Q'j Titrant normality and multiplier determination: Jrn-c-Normality Deionized Titrant _R .. mn End water reference standard ml ml 4.5S.U. number number L b I d a oratolJl contro stan ard: Reference standard True value Sample number (TV) volume Begin (mg CaCO)!L) (m!l ml tc NS.S L:> nti-100 100 "k atriX spz e recovery: Reference standard Spike value Sample number (SV) volume Begin (mgCaCO)!L) (ml) ml IDO 5D .... Sample alkalinity (B) (mg CaC0 3/L) 53 S I amp*e measurements: Titrate samples to pH= 4.50 S.U. Time initiated ____ ---i Time completed Total End ml 1:}-:{., End End ml $)5 Normality (N) ofH 2 S0 4 pH Factor or Multiplier -(5 ml Na 2 C0 3 x 0.05)/E (Nx 50000)/ 100 ml sample "-25/E Nx500 (acceptable range 0.0180-u.w;.w, i Total ml ct:z... Total ml 1-Ho l?) Alkalinity (MV) % RS=MV /TV X 100 Multiplier (mg CaC()JIL) (acceptable range =90 to 110%) l0-1-Alkalinity %RPD= Multiplier (mg CaCOJIL) {(S-D) /[(S+D)/2]} x 100 (acceptable range=+/- 10%) Spike alkalinity (A) Multiplier (mg CaCOJIL) 10:1-G)g Measured spike value (MV) % R = MV I SV x 100 MV=A-B (acceptable range (mg CaC03/L) = 75 to 125%) 40 Joo0"1b Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC03/L) 1 -wri VJBn I t-z_ J 60 3o.S 1 261 .LJ..(Q l2 .. 1 o.--=t-I 2. 34.$ 4.'-t_Lt '1Ll-ol\ us.o&-. *'"L.. J 3 ?,9S J..f?,.?, b <6\ 0<1n41;.r..4.0"=>> 1..fA vuBn Jl2tn1 1 1.!-\-;_:s \.0[7... 1'"\" 0'50\o * 'tl I 2 L t> t1. c?\ J 3 [L\-S_ 40.'2-D .9_ L7 ICl --:.w41A)0f\} uv j)--------* -*-IDI --bSOt.l tiC:. ) lfu'W rw.--9D-9 J4.-::t_a 2 0 _q "+!+ L2 r-------<1t.t ' ...........- \1 ... J 5.3 q.\ Sl ... I age 67 of 107 Reviewed by:

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  • I I I I I I -' I ' ' ' ' I .,.
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  • 1(1) i .} Environmental Tming Solutions.

Inc. Alkalinity (SM:,2320 B) Matrix: Waier;-Ri = 1.0 mg CaC0 3/L Titrate samples to pH= 4.50 S.U. Page Page lq of__,lo'--- Time initiated I'::::-=---,__ Time completed 1----'=------Pc=-flr-----i Titrant normality and multiplier determination: .. _, .. Nnrmalitv .. Deionized Titrant check Begin .l!.na water reference standard ml ml -4.5 s.u. number number L b I d d a oratory contro stan ar : Reference standard True value Sample number (TV) volume Begin (mg CaCO;,IL) (ml) ml lJN'SS \oV)L-} 100 100 q,' Sample ID M.. .k atrtx SPI e recovery: Reference standard Spike value Sample number (SV) volume Begin (mg CaCO;,/L) (mil_ ml S NS. <:) '-o5 '+ IOO 50 c5/1).0 1V'401 ml (E) End ml it End ml ,;(tQ .o Normality (N) of H 2 S0 4 pH Factor or Multiplier ft::. ml Na,C03 X 0.05)/E (Nx 50000)/100 ml sample 0.25/.1!. A.-Nx 500 (acceptable range = 0.0180 -0.0220) Total Multiplier ml cv3 lb:-1' Total Multiplier lc.1 Total Multiplier ml l.I>.u b.\ ID.1-Alkalinity (MV) (mg CaCO;,/L) 1-oo Alkalinity (mg CaCO;,/L) s D % RS = MV I TV x 100 (acceptable range = 90 to 110%) %RPD= {(S-D) /[(S+D)/2)} x 100 (acceptable range=+/- 10%) Spike alkalinity (A) (mg CaCO;,JL) 130 Sample alkalinity (B) Measured spike value (MV) % R=MV I SVx 100 (mg CaCO;,IL) MV=A-B (acceptable range (mg CaC0 3 1L) =75 to 125%) 2>4 q(.p qltcrw s, I amp* e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO:YL) 01\ 0$0(... 'l.'i. -r:JA l/JBf) 'I] INW l a) ld-1-.0 ro l-t lb.1-d\ o l'!l j 2 so L:J'i?. '2.. .1*2.. Lz. .:<tt. oc:; e, s CT'l. o-s £12m-00! 50 I..?SLL) 54-0'\0SCJ1* 0'...:. eJZ.lfn \JD l)_Q l .30.1-81-o4\oscn .en E:F-m {)02_ 5.\ Ltt) Ol0\0 ......... 0'\0Scrl* &.ll-YY'-uoW 2-100 -,::l, 10. \p _v 110 os-01*o16 f------* --59 IY\ HS \fV r::::; JDO ,;}'.;_g. ---=""" --.. -.. 'age 68 of 107 Reviewed by: Date reviewed: I 05 -10 oCJ I I I I I I I I ' I I I I I -I Environmental Testing SoluUons, Jnc. Alkalinity (SM2320 B) Page -5t) Page :1 of 3 --=---Analyst I &:,!. Matrix: Water, RL = 1.0 mg CaC0 3/L Date analyzed 1-o-c.""':::;""'- ,""';'--. D-j-,----1 Titrant normality and multiplier determination: pH of Normality Deionized Titrant check water reference standard == 4.5 s.u. number number 5.0 rNe,32.9 J'NSS\p5'3 B\ k:. o.o -=J c *I L b t I d d a oratory con ro stan ar : Reference standard True value number (TV) (mg CaCOJIL) S NS "S \D 5!...1-100 Sample ID mtts tho J Duplicate (B) M, t' 'k a rrx sp1 e recovery: Reference standard Spike value number (SV) (mg CaCOJIL) I.e 54 50 Begin End ml ml t:.l 1\.lc Sample volume Begin (ml) ml 100 II lo Sample volume _L Sample volume Begin (ml) ml 1\:;t:: '6 Sample alkalinity (B) (mg CaC0 3/L) too S I ampJe measurements: Titrate samples to pH = 4.50 S. U. Time initiated I 1 S Time completed 3....,5=..:.....ol'-------1 '---=-"-----___) Normality (N) of HzS04 pH Factor or Multiplier Total == (5 ml Na 2 C0 3 x 0.05)/E == (N x 50000)/ 100 ml sample ml = 0.25/E ==Nx 500 (E) (acceptable range= 0.0180-0.0220) II.'() r+ ID.9 Alkalinity (MV) % RS = MV I TV x 100 End Total Multiplier (mg CaC03/L) (acceptable range ml ml = 90 to 110%) "'1)3 '6.1--I D-9 <:15 Alkalinity %RPD= End Total Multiplier (mg CaCO:IL) ((S-D) /((S+D)/2]} x 100 (acceptable range=+/- 10%) 0.0 Spike alkalinity (A) End Total Multiplier (mg CaC03/L) ml ml ,3(.?.0 \0.'2.. /C'.C! }10 Measured spike value (MV) % R = MV I SV x 100 MV=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) .-i:rt).&;.c 50 !Oo01o Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOy'L) 05'13-CP.I mtts t-11-0 IOO -41.5 5.S IC:..q ((I(\ ()S. -l I 4lS ,J.i1, l 5.\o G?l eftA (r)\1 .s. uv I 5.lo '5\.p G1 OS*Cfll*d\ & z. II o 5'+ !54 0'5 -n. j 3 ll 0 l!R 5 r::::.r-:1 (aO Z-c;P) SaQJSW I r::. \t ::; .i q 100 L ----d"W-:1 .. 35'}----H-<:J-- -.r.:-o \n ih.Aac::...n ., n n I.'J ')C rl. '0 '\ "\ ., rt -"'-':JI"" ::>\ o't \ w 0<;d ( .2-... 3S.! 'age 69 of I 07 Reviewed by: !-J.lu-3.5 (I *-3g -I Date reviewed: f-(§' Page --"3"-9-L-_ Page cQ of---=3"---- !) Environmental Testfng 5olutlon.s, Inc. Matrix: Alkalinity (SM23:20 B) Water, RL = 1.0 mg CaC03/L Analyst I Date analyzed --i Titrant normality and multiplier determination: pH or -"' ality "" Deionized Titrant check e., ... ..E.tuL water reference standard ml ml 4.5S.U. number number L b I d a oratory contro standar : Reference standard True value Sample number (TV) volume Begin (mg CaCOJIL) (ml) ml :r 1-JSS\.Q 54-100 100 b.O M "k atnx spt e recovery: Reference standard Spike value Sample number (SV) volume Begin (mg CaCOJfL) (ml) ml Fj() 100 14-.'2... Sample alkalinity (B) (mg CaC03fL) 5d--measurements: Titrate samples to pH= 4.50 S.U. Time initiated I I Time completed . Total ml (E) End ml CiH End ml ):3.1 Normality (N) ofHzS04 pH Factor or Multiplier = (Nx 50000)/100 ml sample = (5 ml Na 2 C0 3 x 0.05)/E -0.25/E -Nx 500 (acceptable range O.Ul!!U-v. Total ml Total ml 8.9 Alkalinity (MV) % RS= MY /TV x 100 Multiplier (mg CaCO;VL) (acceptable range = 90 to 110%) ID.q 102... \D"2 C7.p Alkalinity %RPD= Multiplier (mg CaCO:VL) {(S-D) /[(S+D)/2]} x 100 (acceptable range=+/- 10%) t'O*CJ s J_ D Spike alkalinity (A) Multiplier (mg CaC03/L) jD.q Measured spike value (MV) % R = MV I SV x 100 MV=A-B (acceptable range (ml! CaC03fL) =75 to 125%) C1n.o*l Date reviewed: I 05 -1 't D I _] ' --------Environrf'utntal Testing Solution$, Inc. Alkalinity (SM 2320 B) Page LtD Page 3 of 3 ---Matrix: Water, RL = 1.0 mg CaCOiL Date analyzed .. Titrant normality and multiplier determination: ,u D': . d -eiOmze Titrant checK End water reference standard '-mt =4.5S.U. number number L b a oratory control standard: Reference standard True value Sample number (TV) volume Begin (mg CaC03/L) (ml) ml INSSlos4 100 100 D.D Sample volume Begin ml 3 -1 11.'1-atnx sp1 e recovery: Reference standard Spike value Sample number (SV) volume Begin (mg CaCOJIL) (ml) ml :r 054 iDO 50 11.1-Sample alkalinity (B) (mg CaCOJIL) $1-' measurements: Titrate samples to pH= 4.50 S.U. Time initiated I Time completed Normality (N) of H 2 S04 pH Factor or Multiplier Total = (5 ml Na 2 C0 3 x 0.05)/E = (N x 50000)/ 100 ml sample .I = 0.25/E =Nx500 ........__ = 0.0180-0.0220) (E) -----1----0.<1 "'-' Alkalinity (MY) % RS = MY I TV x 100 End Total Multiplier (mg CaCOJII,) (acceptable range ml ml = 90 to 110%) '1.2. q.'l lv.q )oo Alkalinity %RPD= End Total Multiplier (mg CaCOJII,) {{S-D) /({S+D)/2]} x 100 ml ml (acceptable range=+/- 10%) Spike alkalinity (A) End Total Multiplier {mg CaC03/L) ml ml I 't::t 1-.D b lt .. q ) 50 Measured spike value (MY) %R=MV/SVxiOO MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%) cto"W End Total 71 of 107 Reviewed by: Date reviewed: J <ill/ 1 1 Oj w I I I I I I I I I I I I I Analyst I f>>SL Date analyzed 05 -10. bC) Total Hardness (SM 2340 C) RL = l.Orog CaC03/L _-_ --*-,r" Fo Page Page j_ of....cl!J=:-._ Time initiated I Oc:fo<t-Time completed Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDT A pH Factor or Multiplier reference standard ml ml ml =0.2/E = (N x 50000)/ 50 ml sample number number (E) (acceptable range= 0.0180-0.0220) =Nx 1000 lrNRcoz. J:I\JS551-I D.-D J{).O to_o o.v2.0 L b I a oratory contro stan ard: Reference standard True value Sample Hardness (MV) % RS=MV /TVx 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJIL) (ml) ml ml ml =90 to 110%) [Il-JSS(Q5{p 40 50 /D.O 12.0 2.D.O Hc.'D IDoVto Hardness %RPD= Multiplier (mg CaCOJil,) {(S

  • D) /[(S+D)/2]}

x 100 2'U .o s D atri.x soike recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCQJIL) (ml! CaCQJIL) (ml) ml ml ml !INS'S> l-10 5o !Ldp G;,_q 21)_ \) tJ..i-0 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJil,) MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%) Cp--}. .,t.{tp I 1501.o s, I amp* e measurements: I Sample volume Begin End Total Hardness Sample number SampleiD (ml) ml ml ml Multiplier (mg CaCQJiL) I TV=ND Blank PO (should be= 0 rng CaCO:VL) too ;(3 .s {).0 20.0 OS.oq f))MS idlb.l --=liL q7_ I 05.D". *OC>{b ..1 1.28.1 14-.Y-'2g q SSv.J ia5 ,)_;).. .Y.4 }"\ I . .., c J/c ' J' I I " ,'5-J_7 [:+/-. :::> 'r\) n 1-o5-IY1 R m li c:... t-lz)) ?,q '1 IL8.'l t-J .. f} 6'5 *05.0'1 t6 f-Ji6,q J...ftl!+ L4-.IJ ao ---*-qq: ----CP-u OJ) 4<1 If' :" -- '-\Z.. l -::l k_B;6.0 5.\3 _j,_ 12\l , ::y. u 6i:;;rl.n:f'J'*-I* 0 1 + '-' u 1\.J *0>{7 ** abe t :If >15m! of titrant is used, sample must be diluted. Reviewed by: I 1'\Ci;v J Date reviewed r {)6 -10.0'1 )\...l. wei. . 1 I I I I I I I I i I I I I I I I I I .. 1 Total Hardness (SM 2340 C) RL = LO m,g CaC0:/1 --_..:.*,r Page lftJ Page ofl.o..l:::___ Time Time completed Titrant normality and multiplier determination: Titrant Normality check Begin End Total reference standard ml number number L b l d d a oratory contro stan ar : Reference standard True value number (TV) (mgCaCOYL) rrN<-._;::, lQs-v, 40 Duplicate (B) atru; spike recovery: Reference standard Spike value number (SV) (me: CaCQ:VL) Il-J'SS to 5{_p J.+o Sample volume (ml) 50 Sample volume Sample volume (ml) 5o ml (E) Begin End ml ml ;?3.0 6}5. \ End Begin End ml ml :t,.'} Normality (N) ofEDTA pH Factor or Multiplier \3C Total ml Total Total ml 6.3 =0.2/E (N x 50000)/ 50 ml sample 0.0180-0.0220) Nx 1000 Multiplier

2.0.0 Multiplier

\).0 Multiplier 21::1,0 Hardness (MV) (mg CaCO:VL) Hardness (mgCaCOYL) s D lA .., ..... % RS = MV J TV x 100 (acceptable range =90 to 110%) %RPD= {(S-D) /((S+D)/2)} x 100 Spike hardness (A) (mg CaC03/L) 110 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOYL) MV=A-B (acceptable range (mgCaCOYL) =75 to 125%) leLJ? J-.t'-t lto\>Lc s l amp, e measurements: Sample volume Begin End Total Hardness Samole number Sample ID (ml) ml ml ml (mgCaCOYL) Blank ,., u1a be 0 mg CaCOJIL) o"' en. (), A-Pw z. 5o 33.1-3.\ loD 02-. o L-J 3 P1'l-'f' z_q f-)'8 D, I Yr.*+ 5li-IQ I 'z '-1J4 m_q c<.S ! SD 0'\ 0 !:lOt; . J 3 H-hl _d/5 SD C'\ 4* "'2.\* () q 'IY\HS V \J I 0.0 IJ.-fS. qo ---t---C'--\* CJ"\ I 2-l.t-+.S y_*z, q4 ()S-oS-0'\ A I ,;J q,,..L-13."} 14.CS f.\ J c.t ----- f'6-1.. !-+S Cib Date reviewed I OS .Oj 'I >';'f i I I I I I I I I I I I I I I Analyst I tbsc Date analyzed 05 \0. OCf Total Hardness (SM 2340 C) RL = l.O:!!Jg CaC03/L .::: __ .:-.:-;;:- ' Page _91 __ Page .3 of_Lo=--Time initiated , ............. Time completed Ti 'itrant normality and multiplier determination: Titrant l'llll .-h .... k Begin End Total Normality (N) of EDT A pH Factor or Multiplier reference standard m1 -ml ml 0.2/E (Nx 50000)/ 50 ml sample number number (]!;) range= 0.0180

  • 0.0220) = Nx 1000 ----------_Kv a oratory contra stan ar : Lb I d d Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCO:YL) (ml) ml ml ml =90 to 110%) t11J:;s IQ.=5lP 40 50 t-.2. 20.0 4u lODt>zc. Hardness %RPD= End Multiplier (mg CaCOJIL) {(S-D) /[(S+D)/2]}

X 100 a rtx spt e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO)!L) l_mg CaC03/L) (ml) ml ml lrtJss c..os Ll' f>O )o_.n. ..s:J. lR-'2.. (2 I) 2o.u ;<5o Sample hardness (B) Measured spike value (MV) % R=MV /SVx 100 (mg CaCO:YL) MV=A-B (acceptable range (mg CaCO:YL) = 75 to 125%) B() I s, I ampJe measurements: Sample volume Begin End Total Hardness Sample number SampleiD (ml) ml ml ml Multiplier (me CaCO:YL) TV ND Blank i. s ould be 0 mg dl\OSOI* 0'1-(ljj(l{L & t.. .P8 3.5-Jf Czi 2-D.O ) ID ld'lc':>>o'\. ot.o 4 3 BS :2,9-,,?., 0-d1o'SOS. c \ Cha1i IUI!lt) t:;Oi. I JS_ 4-1.l.? '4fl.-:l. l3o opt os<r1. o \ I v 1-. as J-{5 Cj "-1-4.<3 3C1 (J.\ I ..3 a.:; t).t> ;;,-::} 3.1" \5{) 011 oS ()'-\ . Cl \ 7/A 6Rr I JIS 3 ,':f-4.'9 o,; 1Gt0 *.,. IOfl.., --* -* ---z:.. -.-,---* 1-f.q ('i --lqv-cYtoSOI.>.l"( ts.S 1?-.J.t o"'OSOi* Of _J i iK ,\ .Y...:} IJ-lctO \0'(') -L-()'\ OSO"\. 01.._ (.J A lnf f <>J L'tS. i 31-b./) 15D -:-I l.\ -6 lage 74 of 107 . . *-NOte: If >ISm I of titrant ts used. sample must be diluted. Reviewed by: Date reviewed [ 0 "S . i(). (fl I I 1 I ' '.! I ) I : I ! I I ' I I Analyst I -* Date analyzed =5"-l.t)*Qq Total Hardness (SM 2340 C) RL = 1.0 CaC03/L Page 1{ Page i-f of b Time initiated I '>.. Time completed 1-__::""'::::;:o:::--. Titrant normality and multiplier determination: Titr.ant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference stanuaru ml ml = 0.2/E = (N x 50000)/ 50 ml sample number number lEi-range= 0.0180-0.0220) =Nx 1000 --L b J d a oratory contro stan ard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJII,) (acceptable range (mgCaCO;VL) (ml) ml ml ml = 90 to 110%) 'TlJ;:S tonto 40 50 1.2 \.'6 kH.ta 2.'U. '() J-.J.D.(J fOOO"'Zp Hardness %RPD= Total Multiplier (mg CaCOJII,) {(S-D) /[(S+D)/2]} x 100 'k atrrx spz e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (me CaCOJIL) (ml) ml ml ml BD 51+ cz. 2{).() ..Qo1o Sample hardness (B) (mg C.aCO;VL) Measured spike value (MY) MV=A-B (mgCaCO;VL) % R = MV I SV x 100 (acceptable range \l-tD Sample measurements: Sample number Sample ID TV *@_ Blank u<::

  • u mg l aC03/L} ol\ ()So g. -1J A &-f: I f'lt 3 o'\ Cl !:.o'-1 . I{Avv&N 101 ' o'\ oso"".-z.1 I 2. = 75 to 125%) Sample volume Begin End Total (ml) ml rnl ml Multiplier Hardness (mgCaCO;VL)

I '+o --i--Lage 75 of107If Sml f. . . R . db )lt. ., ]\!ote: >I o titrant ts used, sample must be dtluted. evtewe y: "v 11/ L.--'--'--"----__j Date reviewed I 0 6 -\0 1fJ ] i I I J ,,

  • I * --r-----!""' i * * 'Ill 'li'1 :(i}

-.VI-.ro-nm-en-ta-1 Ttiti ___ ng-So-lut-lon-s.l-nc. Total Hardness {SM 2340 C) RL = 1.0-mg CaC03/L ?7 Page '-f<;" Page 5 of Co Time initiated I ':......., Time completed Titrant normality an d multiplier determination: Titrant n End Total Normality (N) of EDT A pH Factor or Multiplier reference standard ml ml ............. 0.2/E -(N x 50000)1 50 ml sample number number <ii) (acceptame

  • 1\ llll!O-0.0220) Nx 1000 1 T L b I d, d a oratory contro stan ar : Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJiL) (ml) ml ml ml =90 to 110%) :TtJSS \oSI., 40 50 1:3.5
)..O 2o.D L-lo £Do0"-u Hardness %RPD= End Total Multiplier (mg CaCO.,IL)

{(S-D) /((S+D)/2)} x 100 M, lx 'k atr spz e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (mg CaCO.,IL) (ml) ml ml ml trN3""(o Sl.P l:> .'Jr-. f;t::t l'z Z..D.u l'lD . Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCO.,IL) MV=A-B (acceptable range (mg CaC(h!L) =75 to 125%) C\;l.. 1ro s l ampje measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO.,IL) TV '.@ Blank II _v1115 L..aL..VJ/ LJ CA o-5 0'-1. ot.. ""Qit wBfliiZ..If)i I as D.9Cz D ?,-(, L z ...:U.'6 .J3.(p 32.. I osul. 1.3 J .3 &4.1. D.\.+> (z c;!Y 0 '\OS 0 '-\

  • 0" r .u RIU 11z. m e<£.1 lcn..o u 110 -;.112-I 2 t'b \00 -: 10 '1 a'\ osocf. 12... j .3 /)O.J.o r., 92. -... -

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  • tl Solutions.

Inc. Analyst I B ----Date analyzed l)]ft 1 tHJ<='\ t* Total Hardness (SM 2340 C) RL :=' CaCOiL Page 1'f::> Page Le Time initiated I '->.. Time completed t--""'-SJ;;o:::::-----i Titrn .. t-.JJ. and multiplier determination: Titrant Normality. check Begin .t;nu A reference standard ml ml ml number number (E) L b I a oratory contro stan ard: Reference standard True value Sample number (TV) volume Begin End (mg CaCOJIL) (ml) rnl ml II:NSS (pr;)lf 40 50 2s-+:1_ Ph2 M. . "k atriX sp1 e recovery: Reference standard Spike value Sample number (SV) volume Begin End _ (mg CaCOJil,) (ml) ml ml 3D 3q,q -454 Normality (N) of EDT A pH Factor or Multiplier -,U * (acceptable range-0.0180 -0.0220) (N x 50 ml sample = X 1000 Total Multiplier ml 2.D_O Multiplier 2{).0 Total Multiplier ml 5. St:z D 2-tl.U Hardness (MV) (mg CaCOJfL) J-ia Hardness (mg CaCOJIL) s H5o D % RS=MV /TV X 100 (acceptable range =90 to 110%) too07....o %RPD= {(S-D) /[(S+D)/2)} X 100 Spike hardness (A) (mg CaCOJfL) O?ao Sample hardness (B) Measured spike value (MV) % R = MV I SV x I 00 (mg CaCOJil,) MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%) l50 1D am_pJe measurements: s l Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (m2CaCOyL) TV= Blank II. (should be= 0 mg CaCO:JLl OS 11'\.0I. lelUn vo 001 50 5D.D lf.lo )0.0 qL. ol\oscn.a-t Oaz.. /0 fil I qq l5) otqo o'\oscn. ElZ.t'n 00002-.50 J.B.o 33Q 5.8 .... J ... \ 'l:O 0<::.-U1-JYv\-iS \f-v'S J_ oo.S 313,.)_ .-J--u ,..,........... v I 1\k------*------\ .....--------=::: l aoe 77 of 107 . _ "" l'lote: If >15m! oft1trant IS used, sample must be diluted. Reviewed by: Date reviewed [ 0'1-(D .Cfj I

  • I I I I ' ! I I I I I l I I _, Analyst bSL 1"--"'""-------1 Date analyzed OS--I1--D 1 Total Hardness (SM 2340 C) RL = 1.0 mg CaC0 3/L Page Page _1-'-'--_of 3 Time initiated I D'15'1 Time completed

..... .. T. itrant normality and multiplier determination: Titrant Normality check Begin End reference standard ml ml number number ..... 0.0 rv D L b l d d a oratory contro stan ar : Reference standard True value Sample number (TV) volume (mg CaC031L) (_ml) *:r)X,S \a54<-40 50 'k atrrx sp1 e recovery: Reference standard Spike value Sample number (SV) volume CaCOJIL) (ml) JNS51.t5ti' 5o Sample hardness (B) (mg CaCOJIL) 92-s l amp. e measurements: Total ml (E) IV.D Begin End ml ml ID.O 11.0 Begin End ml ml .;}15 Normality (N) of EDTA pH Factor or Multiplier =0.2/E = (N x 50000)/ 50 ml sample (acceptable range= 0.0180 ... 0.0220) =Nx 1000 0-Dd.O Total Multiplier ml d),t:> 0(1).0 Multiplier 10.0 Total Multiplier ml \o .. tp 2D, 0 Hardness (MV) (mg CaCOJIL) .L..i0 Hardness (mg CaCOJIL) s D .;?o.o % RS = MV I TV x 100 (acceptable range = 90 to 110%) (DdJLv %RPD= {(S-D) /[(S+D)/2}) x 100 Spike hardness (A) (mg CaC0JIL) f30 Measured spike value (MV) % R = MV / SV x 100 MV=A-B (acceptable range. (mg CaCO:JL) :75 to 125%) :33 Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg_ CaCOJILl TV=ND Blank 5o .;<8.1 JB.I J_o.o NO (should be: 0 mg CaC03/L) D.() .. ffi }t<,. \-!w l-;)i3.1 :9-.0 ql.p OS.ttp.l)C) J ,J-;; .Cl 3J4 J-+.5 qo mH-s OV I A.4 ill'i

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l4!c.J{ .Lfl.o '12.. OS.I2-..Q5 J 3 0.0 :.+.lk- '-' FbJ..wcud J8 *. o\ J 1-f.lp c,.c l.t.J .. --- { b I-* *---I {I i-:-G-4'D 1 1 qo., . if\ I ;Jl L\.2 \)qt1§3--\t rY\Art. shCt Ll t.U-JTf _..;.--I\).\ t;?.'i) ;{ .. l-54 l ge 78 of 1 lmte: If >15m! of titrant is used, sample must be diluted. Reviewed by: Date reviewed I 05 i '1-*rY1 J I I I I I I I I I I I I I ' ' Analyst I &S<..--* Date analyzed 05. 11 . Oj Total Hardness (SM 2340 C) RL = 1.0 mg CaC0 3/L Page Page _;) __ of 3 Time initiated I --------I Time completed Titrant normality and multiplier determination: Tit.:A.nt_ Normality check Begin End Total Normality (N) ofEDTA pH Factor or Multiplier reference standar ,, ml ml =0.2/E = (Nx 50000)/ 50 ml sample number number I acceptable range-0.0180-0.0220) -Nx 1000 Lb d d a oratorv contro stan ar : Reference standard True value Sample Hardness (MV) % RS=MV /TV X 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJIL) (ml) ml ml ml =90 to 110%) ::t"N:::6 40 50 IJ.f.B 0<-D 20.0 looti1.o Hardness %RPD= Multiplier (mg CaCOJIL) {(S-D) /[(S+D)/2]} x 100 ml .2.. 2D.0 s (l.l?l-f-j_ D 4'4 M atrrx spike recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaCOJIL) (ml) ml ml ml It--JGS IQ5t, 4u 5D IB.c !,:)3 .2-5.2 )D.O loo Sample hardness (B) Measured spike value (MV) %R=MV/SVx 100 (mg CaCOJIL) MV=A-B (acceptable range (rng CaC0 3/L) = 75 to 125%) \74* 3lo C\o"l.D S I amp. e measurements: Sample volume Begin End Total Hardness Sample number Sample ID .(ml) ml ml ml Multiplier (mg CaC0 3/L) (should be= 0 mg CaCOy'L)

  • 0 e-IJA 101 z (I) d.3 .)_ Q?(p.3 \ 2_0.\l 0'\ OS IS .Q"'l..-l 3 bo o'\OS I\* o1_ T--IA '60.t-J i NT ( 8d-lt 4--o'\os I 2 3 r:: a.,:) O'i CJS 'S . Ci3 l 3 3.0 t.Qo 0'\(.)$\\.0

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...... cr 1'1., .l 3 llih' ."'. J 1:0"' ') ' j,lj, .. ,)....-o'\o.<<-t\. o'l--v l'¢t4J U\1 1 ,Ji I ' t.\) 3.3 8.3 \..pto 'age 79 of 1 Date: If >ISm! of titrant is used, sample must be diluted. Reviewed by: Date reviewed [ P5-18--oCf I ' I I I ' I I ' I I --,;) Tesring Solutions. Inc. Analyst I i?£i._ *

  • Date analyzed OS-n -D'] Total Hardness (SM 2340 C) RL = 1.0 mg CaCOiL Page '1 '1 Page 3 of-=3=---Time initiated I .______ I Time completed r: r ttrant norma zty and multiplier determinatzon:

'f,W::ant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml 0.21E -(N x 50000)1 50 ml sample number number (]!;) table range-0.0180-0.0220) Nx 1000 -&;c. L b I d d a oratory contro stan ar : Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJil,) (acceptable range (mg CaCOJIL) (ml) ml ml ml = 90 to 110%) ::rw.:=.s 40 50 3.3 5.4-c.< .r 2-1.:>.'0 Lt;l. Hardness %RPD= Multiplier (mg CaC0 3/L) {(S-D) I[(S+D)I2]} X 100 Z.o.o s D atrvc spt e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJil,) {mg CaCOJIL) (ml) ml ml ml .Y.D so '0.5 l3.:t--5.:>.. .z.o.u IDO Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaC031L) MV=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) L?!i-&c C\o<>Lo s l amp, e measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml} ml ml ml Multiplier (mg CaCOJil,) TV=ND Blank .... ,____ (should be -0 mg CaCOJIL) OC\OSIS. If A i..JI Jv 3 6'0 13.1' llo.tJ; )..O.v 5'6 IJ.4. l)..tFQ)j z_g 1/lg.(p Jl..q 5.3 17 I .!Ljz_ 0'\osof-* 2.\ .J ,l l.B rz ,J_ r--*; ____..,. / --L / --( .. -lge 80 of 1 Qdle: If > l Sml of titrant is used, sample must be diluted. Reviewed by: __ K_i.:JA__ ___ _J Date reviewed!.__ -=Q)=--/.__A_-D-"--'1_- --' Page 81 of 107 Sequoyah Nuclear Plant Biomonitoring May 12-19, 2009 Appendix D Reference Toxicant Test and Control Chart

  • * * * --u -l() N u "'0 I t--Pimephales pro me las Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc. Environmental Testing Solutions, Inc. USEP A Control Limits (+/- 2 Standard Deviations) 1.0 0.8 0.6 0.4 1.2 1.0 .U$Ef4 WarningandConttolLimits (75 1 hand9dh PercentileCVs)

.. ---------------*----*-- 0.8 0.6 *-------*-----*--* -**-**--* 0.4 1.2 Laboratory Warning and Control Limits (Idh and 25th Percentile CVs) l.O --*--*--0.8 0.6 0.4 Test date 7-day IC 25 = 25% inhibition concentration. An estimation ofthe concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC 25) Warning Limits (mean IC 25 +/- SA 10 or SA 75) Control Limits (mean IC 25 +/- SA 25 , SA 90 , or 2 Standard Deviations) ""0 \10 !'t> 00 t;J 0 -. 4EETS e-1 * .ol ,* .. , , i.,., .. < *** * .. ,: Environmental Testing Solutions, Inc. Test number Test date 7-day IC,. (gfLKCI) CT (giL KCl) 2 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 10-28-08 11-04-08 12-09-08 01-06-09 01-13-09 01-15-09 01-21-09 02-03-09 02-10-09 02-17-09 02-24-09 02-25-09 02-27-09 03-03-09 03-10-09 03*10-09 04-14-09 04-21-09 05-05-09 05-12-09 Qn QW QW 0.73 on 0.73 0.75 0.75 0.75 0.76 o.n 0.74 0.73 0.73 0.73 0.73 0.74 0.74 0.74 0.74 0.74 0.74 s 0.08 0.06 0.05 0.06 0.06 0.05 0.05 0.05 0.09 0.10 0.09 0.09 0.08 0.08 0.08 0.08 0.08 0.08 O.o7 State and USEPA Control Limits Cf-2S CT+2S 0.56 0.59 0.62 0.62 0.64 0.65 0.66 0.66 0.56 0.54 0.55 0.56 0.56 0.57 0.58 0.58 0.59 0 59 0.59 0.90 0.84 0.84 0.87 0.86 0.86 0.86 0.87 092 0.92 0.91 0.91 0.90 0.90 0.90 0.89 0.89 0.89 0.89 Pimephales promelas Chronic Reference Toxicant Control Chart s._" 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 Laboratory Warning Limits cr-s._,. CT + s._10 0.64 0.63 0.64 0.66 0.66 0.66 0.67 0.67 0.65 0.64 0.64 0.64 0.64 0.65 0.65 0.65 0.65 0.65 0.65 0.82 0.80 0.81 0.83 0.84 0.85 0.85 0.86 0.83 0.82 0.82 0.82 0.82 0.82 0.83 0.82 0.83 0.83 0.83 s..,. 0.15 0.15 0.15 0.16 0.16 0.16 0.16 0.16 0.16 0.15 0.15 0.15 0.15 0.15 0.16 0.15 0.16 0.15 0.15 Laboratory Control Limits cr-s._,. cr + SA.ls 0.58 0.57 0.57 0.59 0.59 0.60 0.60 0.61 0.59 0.58 0.58 0.58 0.58 0.58 0.58 0.58 0.58 0.58 0.58 0.88 0.87 0.88 0.90 0.91 0.91 0.92 0.93 0.90 0.88 0.89 0.88 0.89 0.89 0.89 0.89 0.90 0.89 0.89 s...,. 0.28 0.27 0.28 0.28 0.29 0.29 0.29 0.29 0.28 0.28 0.28 0.28 0.28 0.28 0.28 0.28 0.28 0.28 0.28 USEPA Warning Limits cT-sA.7s cr + sA.7s 0.45 0.45 0.45 0.46 0.47 0.47 0.47 0.48 0.46 0.45 0.45 0.45 0.45 0.46 0.46 0.46 0.46 0.46 0.46 1.01 0.99 1.00 1.03 1.04 1.04 1.05 1.06 1.02 1.01 1.01 1.01 1.01 1.02 1.02 1.02 1.02 1.02 1.02 Note: 7-d IC, = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pil)lephales growth for the test population. CT = Central tendency (mean !C 15). S = Standard deviation of the IC 25 values. Laboratory Control and Warning Limits Suo 0.33 0.32 0.33 0.34 0.34 0.34 0.34 0.34 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 USEPA Control Limits CV CT-SA.Oo cr +SA ... 0.40 0.39 0.40 0.41 0.41 0.42 0.42 0.42 0.41 0.40 0.40 0.40 0.40 0.41 0.41 0.40 0.41 0.41 0.41 1.06 1.04 1.05 1.08 1.09 1.09 1.10 1.11 1.08 1.06 1.06 1.06 1.06 1.07 1.07 107 107 107 1.07 0.11 0.09 0.07 0.08 0.08 0.07 0.07 0.07 0.12 0.13 0.12 0.12 0.11 0.11 0.11 0.11 0.10 0.10 0.10 Laboratory control and warning limits were established using the standard deviation of the IC, values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. Suo= Standard deviation corresponding to the lOth percentile CV. (SA 10 = 0.12) Su 5 = Standard deviation corresponding to the 25th percentile CY. (SA 1 , = 0.21) USEPA Control and Warning Limits s *. , = Standard deviation corresponding to the 75th percentile CY. ( SA.7l = 0.38) s .... = Standard deviation corresponding to the 90th percentile CV. (SA 90 = 0.45) CV = Coefficient of variation of the IC 15 values. USEPA 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003 US EnvironmentaJ Protection Agency, Cincinnati, OH. Organi.Ym.s obtained from Aquatox, Inc. atox05-12-09 Precision of Endpoint Measurements Pimephales promelas Chronic Reference Toxicant Data Environmental Testing Solutions, Inc. Test number Test date Control Control Mean CT cv CT Survival Growth (%) (mg/larvae) for Control Growth (%) for Control (mg!larvae) Growth CV (%) 10-28-08 100 0.586 6.5 2 11-04-08 97.5 0.643 0.614 2.5 4.5 3 12-09-08 100 0.522 0.583 2.4 3.8 4 01-06-09 100 0.644 0.599 3.2 3.6 5 01-13-09 97.5 0.556 0.590 9.7 4.9 6 01-15-09 100 0.707 0.610 6.5 5.1 7 01-21-09 97.5 0.594 0.607 4.9 5.1 8 02-03-09 100 0.671 0.615 8.4 5.5 9 02-10-09 100 0.591 0.613 5.1 5.5 10 02-17-09 100 0.804 0.632 3.3 5.2 11 02-24-09 100 0.744 0.642 2.5 5.0 12 02-25-09 100 0.659 0.643 8.2 5.3 13 02-27-09 100 0.578 0.638 6.8 5.4 14 03-03-09 100 0.664 0.640 8.1 5.6 15 03-10-09 100 0.661 0.642 6.8 5.7 16 03-10-09 100 0.696 0.645 8.8 5.9 17 04-14-09 100 0.671 0.646 10.3 6.1 18 04-21-09 100 0.642 0.646 8.3 6.2 19 05-05-09 100 0.805 0.655 7.9 6.3 20 05-12-09 100 0.717 0.658 7.0 6.4 Note: CV =Coefficient of variation for control growth. Lower CV bound determined by USEPA (lOth percentile)= 3.5o/o. Upper CV bound determined by USEPA (90th percentile)= 20% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference MSD PMSD CT (%) forPMSD (%) 0.10 17.8 0.12 18.7 18.2 0.05 10.0 15.5 0.09 13.3 14.9 0.06 11.2 14.2 0.09 13.3 14.1 0.07 11.5 13.7 0.06 9.6 13.2 0.09 15.7 13.5 0.06 6.8 12.8 0.10 13.0 12.8 0.07 11.1 12.7 0.08 13:1 12.7 0.08 12.3 12.7 0.09 12.9 12.7 0.09 12.6 12.7 0.08 11.4 12.6 0.07 11.2 12.5 0.07 8.4 12.3 0.06 8.9 12.1 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10m percentile)

12%. Upper PMSD bound determined by USEPA (90th percentile)

30%. CT =Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Umkrstanding ami AccoWJting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEP A. 200 la, 200 I b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute \Vhole Effluent Toxicity Test Methods, Volumes l and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtained from Aquatox, Inc. atox05-12-09 Page 84 of I 07 -= -Q ... Co-' -e -Cl Q u -';!. -Cl Q .: *c: '+-< Q .... Cl ... C,j Cj.< 0 u -Q ._, rJ). * * * * *u -E: --... -... Cl ..... ......... b.IJ 6 ._, -= -a: e -0 ... -Cl Q u ... Pimephales promelas Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Organism Source: Aquatox, Inc. 1.00 0.75 0.50 0.25 ----*-*-**-**-*---**-* -* *--*-*----* -**-*-*--*-USEPA Acceptance Criteria(> 0.25 mg per surviving larvae) 30 --*--**-**-**-**-*-

  • -*-----**--*----**-**--*--. Kentucky Acceptance Limit(< 30.0%) 20 10 ******* ..... . ****************

................................

0 30 -*--*---*-* --*-*------*--*-**-**-USEPA Upper PMSD Bound (90th percentile< 30.0%) 20 .....__ 10 Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. --Central Tendency (mean Control Growth, CV, or PMSD) p ge SS of tO'fontrol Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) iETS } Enviranmemal Tasting Solutions-lnc. Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Page 1 of 5 . PpKClCR Test Number: ___iU. Dilution preparation information: KCl Stock INSS number: loL\ \ Stock preparation: 50 g KCL'L: Dissolve 50 g KCI in 1-L Deionized water Dilution prep (mgfL) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Organism age: t.o. 01...1;:) Date and times organisms \loCO were born between: Organism source: &P...\t.\\ 0'5.*\\-Transfer bowl infonnation: pH= SU Temperature= oc "1-4 .lo Average transfer volume: Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding 0 r 2 I 3 I 4 I 5 6 i 7 I I I 86 of 107 Comments: Test information: Randomizing template: Incubator number and shelf location: Artemia CHM number: Date I Time in: o S*t'\*ul\ Date I Time out: Total drying time: Initial oven temperature: Final oven temperature: Test initiation, renewal, l61leeN 2>1:> l-\ l\'-11.\ \1 .. 0b IZ10 lo\

  • t. MHSW batch used iETS ) Environmtnta!Testfng ScHuriDns, Inc. Page 2 of5 Species: Pimephales promelas PpKCICR Test Number: ..J.l1.., Survival and Growth Data Day Control 450mgKCVL 601!_!ng KCVL A B c D E F G H I J K L 0 ID 10 10 /0 IO IO 10 /0 IO /0 10 ID 1 It> 10 If:> to to /0 IC /Q (C) IO IO 10 2 10 I Q 10 I(J 10 ID /0 ID /Q IO 10 IQ 3 IC to ID lO ID 10 10 IC IO 10 10 /D 4 ro /0 to ID 10 10 10 10 IO /0 /0 tO 5 I D 10 10 IO /0 to tO /0 10 IQ 10 10 6 /0 /D /0 10 to IO /0 10 IO /0 10 10 7 /Q IO IO IQ 10 10 /0 ,J.. 10 10 10 10 A= Pan weight (mg) Tray color code:: D ro.!f Analyst: r"L.F 14.11 IJ.D{ j\f. 14.lf.

lVII o .. llJ.qJ Date: OS' *(#-*lA B =Pan+ Larvae weight l'{.l!l> (mg) f\Lr ;).1.4b J.a..lt ttt-.4+ 61 i)o.d-'1 a.t.oJ Jq,q3 Analyst: Date: b 1-b -C =Larvae weight (mg) =B-A 1#. (.;'\ ,.2.\ i.i'5 (a.'\ "Z.. '1.1'2 t,.S't. lo.IY 1-"'H 1. ,g-b.'\1 Weight per initial number v, ,..; ,./ ,_,"11 """"' ,...... oflarvae (mg) 11' .... " ,...rv o-. o-. = C I Initial number of larvae ..., "' \1) ,... " \!1 '\9 '0 \)' o* IV' 0 t> 0' '>. o* Average Percent weight per reduction 0 lo *' '1. 0.11D 1 . () 7. initial from control 0.11"1 number of (%) larvae (m2) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk = sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: $--Comments: age 87 of 107 ' ' ' I I I *Ers *

  • ill' . ) !".:* *.* .*' ..

Page 3 of5 Tit .sting Solutions, Inc. Species: Pimephales promelas PpKCICR Test Number: l& 'L Survival and Growth Data Day 750 KCI/L 900 KCIIL 1050 M! KCI/L M N 0 p Q R s T u v w X 0 10 10 IQ IO /0 10 /0 /() ID /0 10 10 1 &'J.. y.. '{ck. "leA. sJ. SJ,. '{J. ICJ IO /0 \ \., 1 lo s s s 2 l I () 10 lo lo /0 , 1.1 lD 3 & q'c\ l 6 s'cJ. lo 5 lo /Q ro 4 (' &.'J... 10 ,c), '""' ...31J... {'J. '-\ b (0 "' -to 5 :> 5 g-d IC <.::. l.o '-\ '-\ \o \o 6 ft.\. g ; ,(! '\i '!J IV 1'\i 0 o* '\)' \:)' o* o* \Y o* t)' Q' ()' Average Percent weight per reduction 2S. <.. i. S\* &h bl-17. initial from control number of (%) Iarvae(mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: L Comments: I age 88 of 1 07 t.l 110 00 v:; 0 ...... ..... 0 --.J * * * * <] Environmental Testing Solutions. Inc Coac.eotratioa (DIWL Rtlpliute KO) A Control B c D E F 450 G H I J 600 K L M N 750 0 p Q 900 R s T u 1050 v w ----- Dunnett's MSD value: PMSD: Initial aumbcr of larvae 10 10 lit 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 0.064() 8.9 Fiaal .. mbel'of Janru 10 10 10 10 10 10 10 9 10 10 10 10 8 8 8 8 6 5 4 6 3 4 4 4 A -Pu weig*t (mg) 14.77 13.07 14.33 13.05 14.49 14.27 13.31 14.89 13.41 12.75 15.43 12.93 14.44 14.92 14.85 14.01 13.62 13.11 14.76 12.64 14.23 14.11 13.81 15.13 MSD= PMSD= Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses B "" Paa + Larval! L*rvac I (mg) Wci.pt I Suniviat: Mtaa wcielltl Cod&clcat ofvariatioa Weigllt /laitial *umber weiJ:bt(mg) =A.-8 oamher of larvae (mg) Surviviag uuaaber of (M_....,,..,. .. rtlriof oft.rv .. (mg) t.rvae(mg) .....,ruf-".c:)(Ye) 21.46 6.69 0.669 0.669 20.28 7.21 0.721 0.717 7.0 0.721 22.18 7.85 0.785 0.785 19.97 6.92 0.692 0.692 21.61 7.12 0.712 0.712 20.79 6.52 0.652 0.686 3.8 0.652 20.29 6.98 0.698 0.698 21.03 6.14 0.682 0.614 20.82 741 0.741 0.741 19.93 7.18 0.718 0.710 3.6 0.718 22.26 6.83 0.683 0.683 19.90 6.97 0.697 0.697 19.90 5.46 0.683 0.546 19.87 4.95 0.619 0.667 5.1 0.495 20.20 5.35 0.669 0.535 19.58 5.57 0.696 0.557 17.95 4.33 0.722 0.433 16.11 3.00 0.600 0.654 7.7 0.300 17.32 2.56 0.640 0.256 16.56 3.92 0.653 0.392 16.47 2.24 0.747 0.224 17.39 3.28 0.820 0.733 11.0 0.328 16.77 2.96 0.740 0.296 17.63 2.50 0.625 0.250 Minimwn Significant Difference Percent Minimum Significant Diffenonce Test number: PpKOCR#J82 Tell dates* May 12-19, 2009 /(\/v.. ...... 1.. 1 Meaas.,vinJ Meu weiclt.tllaitial Cill!llroeat of Peru.ot rulutioa from (%) lumber of larvae variatioa (%) eootrol (%) (Gig) 100.0 0.717 7.0 Not applicable 97.5 0.669 6.7 6.7 100.0 0.710 3.6 1.0 80.0 0.533 5.1 25.6 52.5 0.345 23.6 51.8 37.5 0.275 16.9 61.7 PMSD is a measure of test precision. The PMSD is the minimwn percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (lOth percentile)

12%. Upper PMSD bound determined by USEPA (90th percentile)

30%. Lower and upper PMSD bounds were detennioed from the lOth and 90th percentile, respectively. ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA. 2001a; USEPA, 2001b). US EPA 200 Ia, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-tenn Chronic and Acute Whole Effluent Toxicity Test Methods, Volwnes I and 2-Appendix. EPA-821-B-{11-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Orgamsms obtamedjrom Aquarox, Inc. atoxOS-12*09 '"Q (10 ('!> \0 Q 0 _, ..... Q -.1 .ETS * * (all 0 *".* ..... -Environmental Tasting Solutions, toe.. Start Date: End Date: Sample Date: Comments: Con e-m giL D-Control 450 600 750 900 1050 *900 *!050 5112/2009 511912009 I 1.0000 1.0000 1.0000 0.8000 0.6000 0.3000 0.5250 0 3750 Auxiliary Tests 2 1.0000 1.0000 1.0000 0.8000 0.5000 0.4000 0.5250 0.3750 Test lD: Lab lD: Protocol: 1.0000 1.0000 1.0000 0.8000 0.4000 0.4000 0.8106 0.6584 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival PpKClCR Sample ID: ETS-Envir. Testing Sol. Sample Type: FWCHR-EPA-821-R-02-013 Test Species: 4 1.0000 0.9000 1.0000 0.8000 0.6000 0.4000 0 6847 0.5796 0.8861 0.6847 11.892 7.979 4 4 Statistic 10.00 10.00 REF-RefToxicant KCL-Potassium chloride PP-Pimephales promelas 10.00 10.00 10.00 10.00 Critical 19 25 Skew Kurt 40 40 Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) Equality of variance cannot be oonfirmed 0.81382352 0.884 -1.2632773 2.14787125 Hypothesis Test (1-tail, 0.05) NOEC LOEC Steel's Many-One Rank Test 600 750 Treatments vs D-Control Parameter Slope Intercept TSCR Pomt ECOI EC05 EC10 ECI5 EC20 EC25 EC40 EC50 EC60 EC75 EC80 EC85 EC90 EC95 EC99 Organisms obtainedfrom Aquatox, Inc. Value SE 95% Fiducial Limits 8.14828973 1.29114829 5.61763904 10.6789404 -19.259728 3.78378062 -26.675938 -11.843518 Probits mg/L 95% Fiducial Limits 2.674 491.787145 380.202898 566.020355 3.355 596.229761 500.029379 659.146411 3.718 660.693371 577.154041 716.766606 3.964 708.079653 634.372816 760.17228 4.158 748.153069 682.308654 798.356768 4.326 784.335178 724.514185 834.704503 4.747 883.459176 829.997827 948.216155 5.000 949.026949 890.476916 1015.58115 5.253 1019.46098 949.626271 1137.82792 5.674 1148.30007 1049.26122 1340.14047 5.842 1203.83407 1090.1848 1431.97553 6.036 1271.96449 1139.27255 1547.85189 6.282 1363.19229 1203.47434 1708.07612 6.645 1510.57893 1304.3074 1978.13621 7 326 1831 38628 1514 60449 2608.95733 ChV TU 670.820393 Maximum Likelihood-Pro bit Control Chi-Sq__ Critical P-value Mu Sigma Iter 0 6.87582293 7.81472778 0.08 2.97727854 0.12272514 4 atoxOS-12-09 "'0 (1Q l'tl -0 ..., -0 ........ ... ,,s ..... .,; ..

  • Statistical Analyses ,) Environmental Testing Solutions, Inc. Larval Fish Growth and Survival Test-7 Growth Start Date: 5/12/2009 TestlD: PpKClCR SampleiD:

REF-Ref Toxicant End Date: 5/19/2009 Lab 10: ETS-Envir. Testing Sol. Sample Type: KCL-Potassiwn chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Conunents: Conc-m!iL I 2 3 4 D-Control 0.6690 0.7210 0.7850 0.6920 450 0.7120 0.6520 0.6980 0.6140 600 0.7410 0.7180 0.6830 0.6970 750 0.5460 0.4950 0.5350 0.5570 900 0.4330 0.3000 0.2560 0.3920 1050 0.2240 0.3280 0.2960 0.2500 Transform: Untransfonned !-Tailed Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.7168 1.0000 0.7168 0.6690 0.7850 7.008 4 450 0.6690 0.9334 0.6690 0.6140 0.7120 6.687 4 1.627 2.180 0.0640 600 0.7098 0.9902 0.7098 0.6830 0.7410 3.567 4 0.239 2.180 0.0640 750 0.5333 0.7440 0.5333 0.4950 0.5570 5.070 4 900 0.3453 0.4817 0.3453 0.2560 0.4330 23.589 4 1050 0.2745 0.3830 0.2745 0.2240 0.3280 16.925 4 Auxiliary Tests Statistic Critical Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97055012 0.805 Bartlett's Test indicates equal variances (E = 0.55) 1.1904434 9.2103405 Test (!-tail, NOEC LOEC ChV TU MSDu MSDp MSB MSE Dunnett's Test 600 >600 0.06396215 0.08923913 0.00265975 0.00172172 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95%CqExe) Skew !COS 608.13 149.72 0.00 651.16 -0.8286 !CIO 642.56 54.75 277.41 687.07 -3.5954 ICI5 676.99 19.21 608.59 724.00 -0.4242 IC20 711.43 18.63 652.82 760.37 -0.2084 IC25 745.86 17.03 695.14 791.43 -0.2232 IC40 832.34 23.14 784.14 931.75 0.9228 IC50 889.53 36.16 825.86 1033.39 0.8216 Organisms obtained from Aquatox, Inc. Isotonic Mean N-Mean 0.7168 1.0000 '0.6894 0.9618 0.6894 0.9618 0.5333 0.7440 0.3453 0.4817 0.2745 0.3830 Skew Kurt 0.28149786 -0.6879762 F-Prob df 0.2649911 2,9 atox05-12-09 f iETS .,j EnvhanmWttil Testing Sokltfcns, Inc. Species: Pimephales promelas 1050 mg KCVL STOCK IPage 92 of 107 Page 4 of5 PpKCICR Test Number: \8"2._ iETS ,!) Envkonmtntaf Testing 5o!U11ont,lnc. Species: Pimephales promelas 1050 mg KCI!L STOCK 93 of 107 Page 5 of5 PpKClCR Test Number: \&-'2-

  • * * ) Ceriodaphnia dubia Chronic Reference Toxicant Control Chart ) Environmental Testing Solutions, Inc. 1.14 USEP A Control Limits (+/- 2 Standard Deviations) 1.12 1.10 1.08 ---------1.06 1.04 1.02 2.5 --u 2.0 USEPA Warning and Control Limits (75th and 9dh Percentile CVs) z 1.5 --In N 1.0 u * * * . -. * * * * * * * * * * ;;;..-. 0.5 "0 I ....... 1.4 1.3 Laboratory Warning and Control Limits (ldh and 25 1 h Percentile CVs) 1.2 1.1 l.O *---0.9 0.8 Test date 7-day IC 25 = 25% inhibition concentration.

An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.

. Central Tendency (mean IC 25) Warning Limits (mean IC 25 +/- SAlO or SA.75) Control Limits (mean IC 25 +/- SA25' SA 90 , or 2 Standard Deviations)

P ". .. 1 ",.. .

  • .,

'"d [10 "" IJI 0 :i * <J I / Test 2 3 4 5 6 7 8 9 10 II 12 13 14 15 16 17 18 19 20 7-day IC 25 CT (giL NaCI) (giL NaCI) 1.09 1.09 1.07 1.07 1.07 1.06 Ill 107 I 09 I 10 I 09 I 07 110 106, 105 1.05 107 107 109 107 1.09 1.08 1.08 1.08 108 1.08 1.08 1.08 1.08 1.08 1.08 1.08 1.08 1.08 1.08 1.08 1.08 1.08 1.08 s 0.00 0.01 0.01 0.01 0.01 0.02 002 0.02 0.02 0.02 0.01 0.01 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart State and USEP A Laboratory Laboratory Control Limits SA.to Warning Limits SA.2s Control Limits SA.1s CT-2S CT + 2S CT-SA.IO CT + SA,IO 1.09 1.06 1.06 1.06 1.05 105 105 1.05 1.05 105 1.05 1.05 1.05 1.05 104 1.04 104 1.04 1.04 1 09 1.11 1.10 1.10 LIO 1.11 1.11 1.11 111 1.11 Lll Ill 1.11 1.11 Ll1 1.11 Ll1 1.11 Lll om om 1.00 1.00 0.99 0.99 0.99 0.99 0.99 0.99 1.00 1.00 1.00 1.00 0.99 0.99 0.99 0.99 0.99 0.99 0.99 1.18 1.17 1.17 1.16 1.16 1.17 1.16 1.17 1.17 Ll7 1.17 1.17 117 1.17 1.16 Ll6 116 1.16 1.16 0.19 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 CT-SA.2S CT + SA.l5 0.90 0.90 0.90 0.89 0.89 0.90 0.89 0.90 0.90 0.90 0.90 0.90 0.90 0.90 0.89 0.89 0.89 0.89 0.89 1.28 1.27 1.26 1.26 1.26 1.26 1.26 1.26 1.27 1.27 1.27 1.27 1.26 1.26 1.26 126 1.26 126 1.26 0.49 0.49 0.49 0.49 0.48 0.49 0.49 0.49 0.49 0.49 0.49 0.49 0.49 0.49 0.48 0.48 0.48 0.48 0.48 USEPA Warning Limits SA.90 CT-SA.75 CT + SA.75 0.60 0.60 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.60 0.59 0.60 0.59 0.59 0.59 0.59 0.59 0.59 0.59 1.58 1.57 1.57 1.56 1.56 1.57 1.56 1.57 1.57 1.57 1.57 1.57 1.57 1.57 1.56 1.56 1.56 1.56 1.56 0.68 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 IC 25 = 7-day 25% inhibition concentratiOn. An estimation of the concentration of sodiwn chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC 21). S = Standard deviation of the ICzs values. I Labdratorv Control and Warning Limits USEPA Control Limits CT-S 00 CT + SA.'!o 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 041 0.41 1.77 1.76 1.75 1.75 1.74 1.75 1.75 1.75 1.75 1.75 1.75 1.75 1.75 175 1.75 1.74 1.74 1.75 1.74 cv 0.00 0.01 0.01 0.01 0.01 0.02 0.01 0.01 O.QJ 0.01 O.Dl O.QJ 0.01 0.02 0.02 0 02 0.02 0.02 0.02 Laboratory control and warning limits were established using the standard deviation of the IC 25 values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint SA.IO = Standard deviation corresponding to the I o'" percentile CV. (SAw= 0.08) SA.lS = Standard deviation corresponding to the 25th percentile CV (SA 23 = 0.17) Control and Warning Limits SA.75 = Standard deviation corresponding to the 75°' percentile CV. (SA 75 = 0.45) SA.'!U = Standard deviation corresponding to the 90th percentile CV. (SA.90 = 0.62) CV = Coefficient of variation of the IC 23 values. and Accounting for Method Variability in Whole Effiuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. 05-05-09

  • * * ,. Precision of Endpoint Measurements Ceriodaphnia dubia Chronic Reference Toxicant Data Environmental Testing Solutions, Inc. Test number 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Test date 01-08-08 01-08-08 02-05-08 03-04-08 04-08-08 05-06-08 06-03-08 07-08-08 08-05-08 09-09-08 10-07-08 11-04-08 11-04-08 12-02-08 01-06-09 02-03-09 02-24-09 03-10-09 04-14-09 05-05-09 Control Survival (%) 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 Control Mean Reproduction CT for Control Mean (offspring/female)

Reproduction (offspring/female) 30.3 31.8 31.1 31.3 31.1 31.5 31.2 29.6 30.9 32.1 31.1 30.5 31.0 30.9 31.0 29.4 30.8 28.3 30.6 32.4 30.7 32.3 30.9 31.5 30.9 31.5 31.0 33.2 31.1 31.4 31.1 32.2 31.2 31.9 31.2 33.9 31.4 33.6 31.5 cv (%) 6.6 4.9 6.4 8.0 7.7 6.3 7.9 6.7 7.9 8.5 7.2 5.1 6.2 4.6 4.0 6.6 6.0 5.8 6.7 4.3 CT for Control Reproduction CV(%) 5.7 6.0 6.5 6.7 6.6 6.8 6.8 6.9 7.1 7.1 6.9 6.9 6.7 6.5 6.5 6.5 6.5 6.5 6.4 MSD 2.2 2.4 2.8 2.7 2.6 2.5 3.0 2.4 2.2 3.0 2.7 2.2 2.4 2.5 2.0 1.9 1.8 2.6 2.0 2.3 PMSD CT (%) for PMSD (%) 7.3 7.7 7.5 9.1 8.0 8.5 8.1 8.8 8.3 7.9 8.2 9.9 8.4 7.7 8.4 7.6 8.3 10.5 8.5 8.4 8.5 6.8 8.3 7.7 8.3 8.0 8.3 6.1 8.1 6.0 8.0 5.7 7.9 8.2 7.9 5.8 7.8 6.8 7.7 Note: CV = Coefficient of variation for control reproduction. Lower CV bound determined by USEPA (lO'h percentile)= 8.9%. Upper CV bound determined by USEPA (90th percentile)= 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (IO'h percentile)

13%. Upper PMSD bound determined by USEPA (90 1 h percentile)

47%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Enviro_tlmentaJ Protection Agency, Cincinnati OH. ______________ _ USEPA. 2001 a, 200 I b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. 05-05-09 Page 96 of 107 -* *

  • Ceriodaphnia duhia .) Environmental Testing Solutions, Inc. Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements

= 35 0 .. --Q,) (J -= 30 "'0 5 0 25 = *c -c. 0 20 .... ...... = 0 0 -u 15 --*-**-Minimum Acceptance Criteria(> 15.0 offspring per surviving female) North Carolina Acceptance Limit ( < 40.0%) Kentucky Acceptance Limit(< 30.0%) USEPA Upper PMSD Bound (90th percentile< 47.0%) 40 30 20 10 Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD the minimum difference between that can be !---declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD) p 97 of 1 0 fontrol Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations) I I I I I I I =t ! . I age 98 of 107 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia CdNaCLCR #: 8'\ Dilution preparation information: NaCI Stock lNSS number: IN$ (.t...( 0 Stock preparation: 100 g NaCl/1 (dissolve 50 g NaCl in 500 ml deionized water) Dilution prep (mg!L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (rnL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test organism source information: Organism age: Date and times organisms were born between: Culture board: o'-\*"2.8-cA Replicate number: 1 1 2 1 3 Culture board cup number: "1.1 I'\ Transfer bowl information: pH ,.'-('\ Daily renewal information: Day Date 0 2 3 4 5 6 7 Control information: % of Male Adults: < 24-hours old os-05-o'\ ot.'-\S Tt>ca5S 4 1 s 1 6 1 1 1 s 1 9 1 10 lO I l"'lj I '-'l ,, I 2\ I n..Ll.l! su Temperature "'l.S* 2. oc Test initiation and feeding, renewal and feeding, or termination time Acceptance criteria 520% %Adults having 3'd Broods: I 00 '1 .. :<:80% % Mortality: O"L $20% Comments: Test information: Randomizing template color: Incubator number and shelf location: YWT batch: SelenrJstrum batch: MHSW batch used Analyst ('} .t At-:6 e_ 2..&\ Summary of test endpoints: 7-day LC 50 )11./00 NOEC &00 LOEC /000 Mean Offspring/Female: 33-'=-:<: 15.0 offspring/female ChV 8<1'-l. ,_, %CV: '-{. 7., <40.0% 1Czs -1 olal. '2-. Page 1 of6 B'f' I. . . . ' it ll IJ i I) I I I I I I i d .,j Species: Ceriodaphnia duhia CONTROL Day 1 1 Young produced 0 Adult mortality '"\ 2 Young produced D Adult mortality L 3 Young produced '0 Adult mortality L-4 Young produced \c. Adult mortality L. 5 Young produced Adult mortality '-6 Young produced 0 Adult mortality L 7 Young produced ,, 2 0 '-a \._ D '-l..\ '-\._ 1\ '--() Total young produced 3(., Final Adult Mortalitv '--\...:_ X for 3rd Broods )(. Page 2 of6 CdNaCLCR #: Survival and Reproduction Data Replicate number 3 4 5 6 7 8 9 10 0 0 D _a_ 0 0 0 0 '-'-\._ L L_ l_ L 0 () a () D 0 0 () '-'-L '--\.._ L L L-D 0 () Cl 0 0 0 0 L \._ \._ L \._ \.._ \.._ L s 'S lo 'S ':::1 \o '-\ \._ '-'-L \...... \.._ '-\._ \£_ \"'2_ 12 '"""' t2 \1.. '-'--'--'-'-'--'-'-0 0 0 D 1\o n \I \S '-L... '-'-L_ '-L \,_ ,, 1'-\ Ho 1\.:, 0 IS 0 a .:!1\ :;'-\ 3:, .3'-\ 3'1-L '---'-'--'---'-L '-)'::.. ")<.. ;><-:>< -,-::: C:_ 'X-Note: Adult mortality (L =live, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: % Mortality: I Dl. Mean Offspring/Female: I .33.1.. 600 mg NCIIL a S . I dR urvtva an d t' D t epro uc ton a a Replicate number Day 1 2 3 4 5 6 7 8 9 10 l Young produced n 0 0 0 0 a 0 0 () 0 Adult mortality L '--L L L L L L \_.. \._ 2 Young produced (J 0 D D 0 0 0 a 0 D Adult mortality 'L L L L L L L L L. L 3 Young produced 0 () a D () [) ll 0 0 () Adult mortality L \__ \._ L L L L L \....__ L 4 Young produced to '-\. 'S. s s lo 'S I..:. \c. 'S Adult mortality '-\..._. L '--L L L L \........ \._ 5 Young produced 17_ 1'-\ \'-\ l'-\ l\ Adult mortality '--'-\...__ '--L. \_ L.. \._. '--'--6 Young produced 0 \ R 0 l\a 0 _Q l&' 0 0 0 Adult mortality L '-\_ \.._ \._ \_ L L L 7 Young produced \l 0 \S 0 18' IS 0 II.. I Ia lb Total young produced 3'5 34 3S d1 3Ll 31o .3$ ..3'-l Final Adult Mortality '--\..._ '---'-'-\.._... L.. "-L '--:I

  • -! emale: Note: Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer).

% Reduction from Control: I age 99 of 107 I I I I I I I \i.Ji I I I l lage 100 of 107 Page 3 of6 Species: Ceriodaphnia dubia CdNaCLCR #: g(\ 800 N CI/L mg a Survival and Reproduction Data Replicate number Day l 2 3 4 5 6 7 8 9 10 1 Young produced 0 a () D 0 n a () 0 0 Adult mortality \.._ L L \.._ '-L L L L '-2 Young produced 0 0 0 0 0 0 () 0 0 0 Adult mortality L L L \_ L L L L \.._ '-3 Young produced D D D 0 a 0 0 0 0 _Q Adult mortality L L L L L-\.._ \._ L L. L 4 Young produced s '-\. q s 'S s \o 'S s Adult mortality L \..._ '-\...._ '--L \.._ \,._ 5 Young produced \:, 1\ \0 I\ \"L l.'l.. ---Adult mortality L L \_ \_ \..__ L \._ \..._ \._. L. 6 Young produced 0 0 0 0 0 0 1'1 0 \lD \lo Adult mortality L '--\_ L L '--L \._. '-7 Young produced ll.o tS 1'5 ,.., IR 0 \S 0 0 TDtal young produced .3'0 3'?:> 3S .2llo .32 00 33 Final Adult Mortality '-'-\.._ \.._ \..._ '--L '-'--'--Note: Adult mortality (L = hve, D =dead), SB = spltt brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer). Conceniration: % Mortality:

07. Mean Offspring/Female: 'Z. %Reduction from Control: \.1.'1. 1000 mg N CI/L a S . I dR urvzva an d t' Dat a epro uc wn Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 D {) a (\ () 0 75 () 0 Adult mortality L \..._ '--L \.._ \.__ '-L L L 2 Young produced 0 0 {) D D 0 0 0 0 0 Adult mortality

\...._ L L L L \_ L \.:... '-L 3 Young produced 0 () 0 0 n C) 0 () n 0 Adult mortality L "-L \.._ L L L \..___ L L 4 Young produced \..to. '-\ lo s I-.\ s s '-\ Adult mortality \...._ L L \._.... L L-L \._ L '--5 Young produced \3 I \ 11.. IO 10 \ \ I\ lL. n ... Adult mortality \..._ \.._. '-\..._ '--'-'--L 6 Young produced 0 0 0 0 0 I( 0 0 0 15 Adult mortality L L L. L L L L L L L 7 Young produced 1:3 IY 12 IS 13 0 II../ l(o 0 Total young produced ..3'2. 2'\ ..30 .3C '21 ..30 3o o-z. .3"2.... Final Adult Mortality L '---\.._ L '-\...._ '-'---c.::-'--Note. Adult mortality (L = hve, D =dead), SB =spilt brood (stngle brood split between two days), CO*-carry over (offspnng carried over with adult during transfer). r oncentr.ation

  • % Mortality:
07. Mean Offspring/Female:

3o:1 % Reduction from Control: /i.,., 1. r r f I I I I I I I I "" I I I ' Page 4 of6 Species: Ceriodaphnia dubia CdNaCLCR#: 1200 N Cl/L mg a I dR Surviva an d epro uctzon D at a -* Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced '() 0 (') 0 n 0 0 0 I) 0 Adult mortality '-\-\-'-L.. l_ \..__ L. '-L 2 Young produced 0 0 () (') 0 (') a (} 0 0 Adult mortality L '-\_ L '-\..._ '-L L.. L 3 Young produced \) 0 0 0 () 0 0 0 0 0 Adult mortality L L L L L L-L L L L. 4 Young produced <. s s '-\ '-\ "S. ..... "-\ '-\ Adult mortality '-'-\._ '--\._ L '--\.._. '-'--5 Young produced s s .., .3 ., s l.n s s Adult mortality '-L '---\_. '-'-'--'--'-\._ 6 Young produced D c 0 0 0 0 0 .... , 0 lP Adult mortality L L. '-'-L. '--\.._. L-L "-7 Young produced ..5 q !,., s l.D y ., .s 0 Total young produced IS 1'1 \& 12 '"' llo t e Jy 15 Final Adult Mortality \._ L L '-L L L L '---'--Note: Adult mortality (L = hve, D =dead), SB = spltt brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: % Mortalitv:

07. Mean Offspring/Female:

1"5.q % Reduction from Control: '52.17o 1400 ID2 NCI/L a S . I dR urv1va an d t* D t epro uc zon a a Replicate number Day I 2 3 4 5 6 7 8 9 10 1 Young produced [") () (') {) () () () 0 0 0 Adult mortality '--L '--L. L L.. L L L \..._ 2 Young produced 0 -o 0 0 0 0 0 0 0 0 Adult mortality L '--L L L L. \... L \.... '-3 Young produced 0 0 0 0 0 0 0 0 0 () Adult mortality L L L L L L L L l_ L 4 Young produced s l.. '-\ "L. Adult mortality \._ \.._. L L L L '-'-L L. 5 Young produced '-\ a .s s \c <.J tJ l.\ Adult mortality L \._ \_ '--L \.._ \._ L L. 6 Young produced 0 y 0 0 0 0 0 £_ \ 0 Adult mortality L L L L L \.._... L L I L 7 Young produced \ 0 0 :s '2.. () 0 w -3 Total young produced l£.. 8 -, 5 l \ '1 5 3 q Final Adult Mortality L L \...... \.._ \....._ L L. L t.> L Note.* Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer)

*--toncetztration.

% 1()1. Mean Offspri.llgffemale:

  • --'1 ...,. beJ\"t>

obC:.e.L>Ieh. o-leA.. -_, % Reduction from Control: IS. 07o 101 of 107 '"d to) (10 ("C -0 N 0 * ....., -. *J _) I Verification of Cerio* *aphnia Reproduction Totals ) En Iron nental Testing Solutions, Inc. ( on rol 1000 mg NaCI/L Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 4 5 5 6 5 5 6 4 4 50 4 6 4 6 5 4 5 5 5 4 5 49 5 13 13 12 12 12 14 12 12 13 13 126 5 13 II 12 10 10 13 II II 12 12 115 6 0 17 0 0 0 0 16 0 17 15 65 6 0 0 0 0 0 17 0 0 0 15 32 7 17 0 17 14 16 16 0 15 0 0 95 7 13 14 12 15 13 0 14 14 16 0 Ill To al 36 34 34 31 34 35 33 33 34 32 336 Total 32 29 30 30 27 35 30 30 32 32 307 NaCl/L 1200 mg NaCUL Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 l 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 41 6 4 5 5 5 6 5 6 6 5 53 4 5 5 5 4 4 5 4 4 4 4 44 5 12 13 14 14 14 13 13 II 13 13 130 5 5 5 7 3 7 5 5 6 5 5 53 6 0 18 0 16 0 0 18 0 0 0 52 6 0 0 0 0 0 0 0 I 0 6 7 7 17 0 15 0 18 15 0 16 16 16 113 7 5 9 6 5 8 6 4 7 5 0 55 To al 35 35 34 35 37 34 36 33 35 34 348 Total 15 19 18 12 19 16 13 18 __ __ 1400 mgNaCl/L Replicate number Total ! I 2 3 4 5 6 7 8 9 10 Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 5 5 5 6 5 5 5 49 4 5 3 4 2 5 4 3 3 3 2 34 5 13 13 II 13 II 12 13 12 12 12 122 5 4 0 3 3 5 5 6 0 0 4 30 6i 0 0 0 0 0 0 17 0 16 16 49 6 0 4 0 0 0 0 0 2 0 0 6 7 16 16 15 15 17 18 0 15 0 0 112 7 3 1 0 0 5 2 0 0 0 3 14 34 33 30 33 33 35 36 32 33 33 332 Total 12 8 7 5 15 11 9 5 3 9 84 '"Q :w [JQ (t> -Q w 0 "-l -Q -..1 Enylro(lmental Testing Solutions, Inc. 2 I 3 34 I 34 35 I 34 34 I 33 I 30 -100(1 I 32 I 29 I 30 -I 15 I 19 I 18 I 12 I 8 I 7 Dunnett's 2.291 PMSD: 6.8 I I I I I I I Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses 4 31 35 33 30 12 5 MSD= PMSD= Test number: CdNaOCRN89 Test dates: May 05-12,2009 Reveiwed by: IPolicate number I Survival I Average reproduction I Coefficient of I Percent reduction from I (%) (offspring/female) variation (Vo) control (%) 5 6 7 8 9 10 34 35 33 33 34 32 100 33.6 4.3 Not applicable 37 I 34 36 33 35 34 100 34.8 3.3 -3.6 33 I 35 36 32 33 33 100 33.2 4.9 1.2 27 I 35 30 30 32 32 100 30.7 7.0 8.6 19 I 16 13 18 14 15 100 15.9 15.8 52.7 15 I 11 9 5 3 9 90 8.4 43.2 75.0 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (!Oih percentile)

13%. Upper PMSD bound determined by USEPA (901h percentile)

47%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (tJSEPA, 200la; tJSEPA, 2001b). USEP A I a,!200 I b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-0 1-004 and EPA-821-B-0 1-005. US Protection Agency, Cincinnati, OH. riO ..... 0 ""' 0 ....., ..... 0 -..1 :

12) ,' . *: __) Statistical Analyses Environmental Testing Solutions, Inc. Ceriodaplmia Survival and Reeroduction Test-R=eroduction 1 Start Date: 5/5/2009 TestlD: CdNaClCR i End Date: 5/12/2009 Lab ID: ETS-Envir.

Testing Sol. Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Corrunents: I 2 3 4 5 6 0-Control 36.000 34.000 34.000 31.000 34.000 35.000 600 35.000 35.000 34.000 35.000 37.000 34.000 &00 34.000 33.000 30.000 33.000 33.000 35.000 1000 32.000 29.000 30.000 30.000 27.000 35.000 1200 15.000 19.000 18.000 12.000 19.000 16.000 1400 12.000 8.000 7.000 5.000 15.000 11.000 Transform: Untransformed Conc-mg!L Mean N-Mean Mean D-Control 33.600 1.0000 33.600 600 34.800 1.0357 34.800 800 33.200 0.9881 33.200 *1000 30.700 0.9137 30.700 *1200 15.900 0.4732 15.900 *1400 8.400 0.2500 8.400 Auxiliary Tests Kolrnogorov 0 Test indicates normal distribution (p > 0.01) Bartlett's Test indicates unequal variances (p = 8.87E-03) Min 31.000 33.000 30.000 27.000 12.000 3.000 ! Hypothesis Test (!-tail, 0.05) NOEC LOEC Steel's Many-One Rank Test 800 1000 Treatments vs D-Control Max CV% 36.000 4.255 37.000 3.262 36.000 4.877 35.000 7.045 19.000 15.814 15.000 43.179 ChV TU 894.427191 Sample ID: Sample Type: Test Species: 7 33.000 36.000 36.000 30.000 13.000 9.000 N 10 10 10 10 10 10 Statistic 0.81542361 15.3749275 Point mg/L SO 95% CL Linear interpolation (200 Resamp1es) Skew IC05 856.8 42.6664107 764.087662 941.198214 IC10 993.6 29.0004806 921.551731 1015.99639 1, JC15 1022.02703 8.689644 1004.45361 1037.76034 1C20 1045.13514 7.64509171 1029.66986 1060.12003 IC25 1068.24324 7.05805813 1055.32988 1081.38741 IC40 1137.56757 7.32664661 1123.52221 1151.44087 lC50 1183.78378 9.03530003 1167.78076 1201.21731 0.0444 -0.8830 -0.3344 -0.0590 0.0234 0.2385 0.2331 8 33.000 33.000 32.000 30.000 18.000 5.000 Rank Sum 129.50 95.50 68.00 55.00 55.00 REF-Ref Toxicant NACL-Sodium chloride CD-Ceriodaplmia dubia 9 34.000 35.000 33.000 32.000 14.000 3.000 !-Tailed Critical 75.00 75.00 75.00 75.00 75.00 Critical 1.035 15.0862722 10 32.000 34.000 33.000 32.000 15.000 9.000 Isotonic Mean N-Mean 34.200 1.0000 34.200 1.0000 33.200 0.9708 30.700 0.8977 15.900 0.4649 8.400 0.2456 Skew Kurt 0.21736896 0.95569016 05-05-09 ""0 (10 ...... Q Ul 0 -. ...... Q ....... *

  • w *6 Statistical Analyses ._) ) Environmental Testing Solutions, Inc. Usc: d for PMSD calculation only. Ceriodaphnia Survival and Reproduction Test-Reproduction Sta 5/5/2009 Test ID: CdNaClCR Sample ID: 1 Enc Date: 5/12/2009 Lab ID: ETS-Envir.

Testing Sol. Sample Type: San pie Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: Co Cc nc-mg/L I 2 3 4 5 6 7 8 10-Control 36.000 34.000 34.000 31.000 34.000 35.000 33.000 33.000 I 600 35.000 35.000 34.000 35.000 37.000 34.000 36.000 33.000 800 34.000 33.000 30.000 33.000 33.000 35.000 36.000 32.000 1000 32.000 29.000 30.000 30.000 27.000 35.000 30.000 30.000 1200 15.000 19.000 18.000 12.000 19.000 16.000 13.000 18.000 1400 1'2.000 8.000 7.000 5.000 15.000 11.000 9.000 5.000 Transform: Untransformed Cc nc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat [ -Control 33.600 1.0000 33.600 31.000 36.000 4.255 10 600 34.800 1.0357 34.800 33.000 37.000 3.262 10 -1.198 800 33.200 0.9881 33.200 30.000 36.000 4.877 10 0.399 *1000 30.700 0.9137 30.700 27.000 35.000 7.045 10 2.895 *1200 15.900 0.4732 15.900 12.000 19.000 15.814 10 17.667 *1400 8.400 0.2500 8.400 3.000 15.000 43.179 10 25.153 Au iliaryTests Statistic Ko mogorov D Test indicates normal distribution (p > 0.0 t) 0.81542361 Bar lett's Test indicates unequal variances (p = 8.87E-03) 15.3749275 HyJ othesis Test ( 1-tait, 0.05) NOEC LOEC ChV TU MSDu MSDp Du nett's Test 800 1000 894.427191 2.29089729 0.0681814 7 Tr4tments vs D-Control ! REF-Ref Toxicant NACL-Sodium chloride CD-Ceriodaphnia dubia 9 10 34.000 32.000 35.000 34.000 33.000 33.000 32.000 32.000 14.000 15.000 3.000 9.000 !-Tailed Critical MSD 2.287 2.291 2.287 2.291 2.287 2.291 2.287 2.291 2.287 2.291 Critical Skew Kurt 1.035 0.21736896 0.95569016 15.0862722 MSB MSE F-Prob df 1241.68 5.01851852 6.4E-36 5, 54 05-05-09 I I I I I I I I I I I I I I I I Species: Ceriodaphnia dubia CONTROL 600 mg NaCI/L 800 mg NaCI/L 1000 mg NaCI!L 1200 mg NaCI!L 1400 mg NaCJIL lage 106 of 107 -Page5of6 CdNaCLCR #: 8'\ I I I Species: Ceriodaphnia dubia Page6of6 CdNaCLCR #: 8q I CONTROL I I ! 600 mg NaCIIL 800 mg NaCIIL 1000 mg NaCI/L 1200 mg NaCI/L 1400 mg NaCI!L 107 of 107 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L _E. Q_BOX..£.900 ___________ _ -__ ...§.ODD.Y_- DAISY _ _Jt::!]738+/-_ ______ _ Facility _ _s:./A -_g':QUOYAH NUCLEAR PLANT _____ _ Locatio.!!._ _!::!AMIL TO.!::!_COUNT:L_ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMRJ TN0026450 I L PERMIT NUMBER I 103 G DAY From 31 MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 LOW VOL WASTE TREATMENT POND EFFLUENT *** NO DISCHARGE CJ *** Read instructions before completinQ this form. , PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE i EX I OF TYPE I AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM ANALYSIS 1 PH 20 /31 SAMPLE ******** ******** 7.6 8.7 ******** 0 I MEASUREMENT 12 i !00400 1 0 0 'EFFLUENT GROSS VALUE PERMIT REQUIREMENT

                • . tMINJMUM'
    ,*r* . .

su jSOLIDS, TOTAL SUSPENDED I SAMPLE . MEASUREMENT ioo53o 1 o o !EFFLUENT GROSS VALUE lOlL AND GREASE I 00556 1 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT PERMir .. -REQUIREMEf.:lt .l: < *;co, 26 ******** 15 19 177 MG/L ...... 12SO l

. :*<* * ....* <122 j50050 1 0 0 !EFFLUENT GROSS VALUE -*._.

MGD ---1------- I 1 DAILY l :,:*;;1 * * .nii -l;:Zh.. I I . l -PERMIT' REQ!JIREMENT NAME!TITLE PRINCIPAL EXECUTIVE OFFICER !I Cert1fy under penalty of law that th1s document and all attachments were prepared under my -----------**---------1d1rect1on or superv1s1on 1n accordance with a system designed to assure that qualified personnel Timothy P. Cleary 'properly gather and evaluate the 1nformat1on submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Information, the information submitted 1s , to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are s1gmficant penalties for submitting false information, including the possibility of fme and impnsonment for know1ng violations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) - d I TELEPHONE t1Cl!L..-U

  • 0-t. ! Prrncipal Environmental Engmeer I 423 843-6700 SIGNATURE OF PRINCIPAL EXECUTIVE r I OFFICER OR AUTHORIZED AGENT AREA I NUMBER CODE The Turbine Building Sump (TBS) was discharged directly to the Yard Pond (YP) on 5/5/09@ 0850-5/9/09@ 1425 and on 5/11/09@ 1625-5/11/09@ 1740. 09 YEAR GRAB DATE 06 09 MO DAY EPA Form (REV 3199) Previous editions may be used Page 1 of 1 PERMITIEE NAME/ADDRESS (Include FaCilitY Name/Location 1f Different)

___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 ___ _§_ODD...Y_- DAISY _ _IN 37384._ ______ _ ..EE_cilitv_ JYA -..§E:QUOYAH NUCLEAR PLANT _____ _ Locatio.!l._ ..J::!AMILTOJ::!..COUNTX,_ _________ _ ATTN: Stephanie A. Howard PARAMETER TN0026450 YEAR From I 09 I n I -* I QUANTITY OR LOADING AVERAGE MAXIMUM UNITS ******** ******** MINIMUM 107 G METAL CLEANING WASTE POND EFFLUENT *** NO DISCHARGE I XX I ... NOTE: Read instructions before completinQ this form. QUALITY OR CONCENTRATION I NO.I FREQUENCY I SAMPLE I EX OF TYPE I AVERAGE MAXIMUM UNITS ANALYSIS ******** 12 i00400 0 0 !EFFLUENT GROSS VALUE !SOLIDS, TOTAL SUSPENDED

                • \ loo530 1 o o EFFLUENT GROSS VALUE OIL AND GREASE 00556 1 0 0 I !EFFLUENT GROSS VALUE 1 PHOSPHORUS, TOTAL (AS P) !00665 1 0 0 ******** ',EFFLUENT GROSS VALUE .* + I 1-----... PERMIT REQUIREMENT

!COPPER, TOTAL (AS CU) 01042 1 0 0 EFFLUENT GROSS VALUE IRON, TOTAL (AS FE) 01045 0 0 EFFLUENT GROSS VALUE ,FLOW, IN CONDUIT OR THRU !TREATMENT PLANT ! ]50050 1 0 0 !EFFLUENT GROSS VALUE SAMPLE MEASUREMENT

  • PERMIT"-':*:*

SAMPLE MEASUREMENT I SAMPLE MEASUREMENT PERMIT REQUIREMENT

'----

REPORT MOAVG NAME/TITLE PRINCIPAL EXECUTIVE OFFICER l Cert1fy under penalty of law that th1s document and all attachments were prepared under my direction or supervision 1n accordance w1th a system des1gned to assure that qualified personnel Timothy P Cleary properly gather and evaluate the information submitted. Based on my 1nquiry of the person or i persons who manage the system, or those persons dJrectly responsible for gathering the Information, the information submitted JS , to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. l am aware that there are significant penalties for submitting false Information, Including the possibility of fine and imprisonment for knowing violations. L__ TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT MG/L :eMs TELEPHONE DATE 423 843-6700 09 06 09 MO I DAY-AREA NUMBER YEAR CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Add res.§__£ ___________ _ -__ _§_ODD_r_- DAISY _ _IN 37384.._ ______ _ Fac11itv_ .I:JA -_§_EQUOYAH NUCLEAR PLANT _____ _ Locatio.!l... _!::!AMIL TOJi.COUNTY __________ _ ATIN: Stephanie A. Howard PARAMETER NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) TN0026450 110 G From 1 __ 1 ---* QUANTITY OR LOADING AVERAGE I MAXIMUM MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT Form Approved. OMB No. 2040-0004

      • NO DISCHARGE I XX I *** NOTE: Read instructions before completinq this form. UNITS NO. lt-t<t:OUENCYI SAMPLE EX OF TYPE ANALYSIS MAXIMUM TEMPERATURE,-WATER

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                • ******** ********

---1 :cENTIGRADE 1 MEASUREMENT ! 1 04 04 !0001 0 Z 0 0 . 1 'IINSTREAM MONITORING I REQiJIREMENT PH I SAMPLE 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED I loo53o 0 0 MEASUREMENT .. / *I> ... DA."'"'"" MX :: .. " , , -..*. su 19 IEFFLUENT GROSS VALUE --!-----::-- I .... >* I ' SAMPLE 1 lolL AND GREASE I MEAsuREMENT 1 ' I, I ioo556 1 o o [EFFLUENT GROSS VALUE !FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL I 150060 1 0 0 ]EFFLUENT GROSS VALUE PERMIT SAMPLE MEASUREMENT .. ,,, * *--.-Hi,**:c:". .:R--e* **P*ORT . . ! .* .* *. /C , ... -***-..*. *

  • SAMPLE MEASUREMENT
                • 1 SAMPLE I I ! I REQUIREMENT
  • .. *. I NAMEffiTLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel I I I I I Timothy P. Cleary properly gather and evaluate the informalion submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the informat1on, the information subm1tted IS , to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalt1es for submittmg false information, including the poss1bil1ty of fine and tmprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV'3/99) Previous editions may be used ) [phW"'-'lL TELEPHONE DATE Principal Environmental Engineer 423 843-6700 09 06 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREA l NUMBER YEAR MO CODE Page 1 of 1 09 DAY PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L _E ___________ _ -__ _§_ODD..'!:_- DAISY_ .....Jt:!.]738i_ ______ _ Facility_ ..JYA NUCLEAR PLANT _____ _ Locatio.!l._ _!:!AMIL TO.!::!.. COUNTY __________ _ ATTN Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMRJ TN0026450 110 T PERMIT NUMBER DISCHARGE NUMBER From MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. I FREQUENCY! SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM IC25 STATRE ?DAY CHR CERIODAPHNIA iTRP3B 0 0 1 EFFLUENT GROSS VALUE IC25 STATRE ?DAY CHR PIMEPHALES TRP6C 0 0 EFFLUENT GROSS VALUE ******** ******** ******** ******** SAMPLE I MEASUREMENT .. , ,. J' . PERMIT REQUIREMENT

  • SAMPLE I MEASUREMENT I PERMIT l 1

..* , *. ! NAMEffiTLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my I direction or superv1sion in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the I information, the information submitted is , to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false 1nformat1on, 1nc!uding the possibi!1ty of fine and impnsonment for knowing VIolations. r TYPED OR PRINTED i __ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous ed1t1ons may be used Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT UNITS ANALYSIS 23 ;cOMPOs TELEPHONE DATE 423 843-6700 09 06 09 I I---I-AREA l NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res.§_ ___________ _ -__ _§_ODD .:I_-DAISY _ _JN 37381..._ ______ _ Facility_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ _!jAMILTOJi.COUNTY._ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 116 G MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 A TIN Stephan1e A Howard NO DISCHARGE D ... NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. \FREQUENCY\ SAMPLE I EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS ******** ******** ******** ******** 0 0 9A DEBRIS, FLOATING (SEVERITY) 1 I 31 01345 0 0 PASS=O EFFLUENT GROSS VALUE I OIL AND GREASE VISUAL 'j .. .. ;r_ FAIL=1 I I SAMPLE MEASUREMENT ! ******** is4066 1 o o f-PERMIT ******** REPORT *;; :EFFLUENT GROSS VALUE I REQUIREMENT MO TO:fA[ *----**---*-----**------- I SAMPLE l I MEASUREMENT PERMIT .. REQUIREMENT' SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT

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RgdUIREMENT ,>;c ;;'?;;_, NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my ;* \ (\ .I/ . d dJrect1on or supervrs1on tn accordance a des1gned to assu.re qualtf1ed personnel "-*'7\pt1ll.JL.U.. .\J...{QI'k_)CU-- Timothy p. Cleary properly gather and evaluate the 1nformat1on submitted. Based on my 1nqu1ry of the person or UC I persons who manage the system, or those persons responsible for gatherong the Principal Environmental Engineer . . . informatiOn, the mtormat1on submJtted 1s , to the best of my knowledge and bel1ef, true, Sequoyah S1te ViCe President jaccurate. and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE '------*Information, 1nclud1ng lhe poss1bility of f1ne and impnsonment for know1ng VIOlations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED 1 COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used TELEPHONE 423 843-6700 AREA CODE Page 1 of 1 VISUAL PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L ___.!: Q_BOX_1900 ___________ _ -__ _§_ODD_.':{_- DAISY _ _It:U738i_ ______ _ Facility_ ....JYA -_g:QUOYAH NUCLEAR PLANT _____ _ LocatiO.!:!_ J:::!AMIL TO..!:!_COUNTr_ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) I TN0026450 -1 1 PERMIT NUMBER [ [ DISCHARGE NUMBER 117 G MONITOR_lNG_EEEUOD MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 NO DISCHARGE LJ ... [YEAR DAY From L()9 To 31 I I ATIN Stephanie A. Howard PARAMETER NOTE: Read instructions before completinq this form. 1 NO. [FREOUENCYI SAMPLE EX I OF ANALYSIS AVERAGE MAXIMUM UNITS UNITS MINIMUM AVERAGE MAXIMUM 1/ "'I DEBRIS, FLOATING (SEVERITY) SAMPLE ******** ******** ******** ******** Q gA 0 VISUAL 01345 1 0 0 !EFFLUENT GROSS VALUE OIL AND GREASE VISUAL 84066 0 0 PERMIT !EFFLUENT GROSS VALUE REQUIREMENT REPOR'(.Stj YES=1 MO TOT At:. NO=O PERMIT. REQUIREMENT SAMPLE MEASUREMENT PERMIT 1 RsouiREr-1ENT. j , j \ " . _ " _j I I SAMPLE I , *'* ?ERMIP ' ,;. REatliREMEr,rr ' > NAME/TITLE PRINCIPAL EXECUTIVE OFFICER II Certify under penalty of law that this document and all attachments were prepared under my Timothy P. Cleary I direction or supervis1on in accordance with a system d. esigned to assure that quali.fied personnel properly gather and evaluate the 1nformat1on submitted Based on my inqu1ry of the person or persons who manage the system, or those persons d1rect!y responsible for gathenng the . . . '[Information. the informat1on subm1tted is. to the best of my knowledge and belief, true, Sequoyah S1te V1ce President ,accurate. and complete I am aware that there are S1gn1f1cant penalt1es for submitting false __________ _J 1nformat1on the poss1b1lity of fine and Imprisonment for knowtng violations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous hOJvi. (). ev--cl Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT VI$UAL VI$,1:JAi.:: 1 TELEPHONE 423 843-6700 06 09 NUMBER YEAR! MO DAY Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres.L_ ___________ _ -__ _§_ODD_'!.: DAISY _ _IN 3738+/-_ ______ _ Factlitv _ _IYA -_.§_EOUOYAH NUCLEAR PLANT _____ _ Locatio.Q_ .J:!AMIL TO.t!._COUNTY __________ _ ATTN Stephanie A Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ MAJOR (SUBR 01) TN0026450 118 G F -FINAL PERMIT NUMBER DISCHARGE NUMBER I WASTEWATER & STORM WATER MONITORlli.G_E'ERIOD EFFLUENT NO DISCHARGE I XX I ... From YEAR-, DAY 09 To 31 Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUALITY OR CONCENTRATION I NO. \FREQUENCY\ SAMPLE UNITS MINIMUM AVERAGE MAXIMUM EN, DISSOLVED (DO) 0 0 LIDS, TOTAL SUSPENDED 0 0 SOLIDS, SETTLEABLE 00545 1 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Sequoyah Site Vice President I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance w1th a system designed to assure that qualified personnel properly gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system. or those persons directly responsrble for gathering the 1nformat1on, the 1nformat1on subm1tted IS , to the best of my knowledge and belief, true, accurate, and complete I am aware that there are significant penalties for subm1ttmg false _____ ______ . ______ ------* _____ _jtnformatlon. 1ncludtng the poss1btl1ty of f1ne and 1mpnsonment for knowing violations TYPED OR PRINTED I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used ******** ******** Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TYPE UNITS 19 TELEPHONE DATE 423 843-6700 09 ' 9 AREA I NUMBER YEAR M CODE Page 1 of 1 .4 S58 090714 800-NPDES CORRESPONDENCE July 14, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 40 1 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JUNE 2009 Enclosed is the June 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of the Sequoyah Environmental staff. Tim::::lleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN R. M. Payne, LP 4B-C A. A. Ray, WT 7C-K G. R. Signer, WT 6A-K B. A. Wetzel, OPS 4A-SQN EDMS, WT CA-K (Enclosure) DMR0906.doc REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JUNE 2009 ORGANIZATION: Chemistry and Environmental DOCUMENT PREPARED BY: Ann Hurt DATE: 07/09/2009 CATEGORY: NPDES Correspondence CONCURRENCES Name R c Signature -Comment Date v N Ann Hurt X Stephanie A Howard X Alan K Barringer X Debra J Bodine X Beth A. Wetzel X Chris R Church X Timothy P Cleary X (for signature)

4) 1 Lt --1 u u::P( INSTRUCTIONS:

Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant July 14, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

Tennessee Valley Authority Post Office Box 2000 Soddy Daisy, Tennessee 37384-2000 SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JUNE 2009 Enclosed is the June 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at ( 423) 843-6714 or Stephanie Howard at ( 423) 843-6700 of the Sequoyah Environmental staff. Cleary Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission A TIN: Document Control Desk Washington, D.C. 20555 pnnted on recycled paper PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Address P.O. BOX 2000 NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT (OMRJ MAJOR (SUBR 01) F-FINAL Form Approved OMB No 2040-0004 ___ _§_ODD..Y_- DAISY _ _IN 37381._ ______ _ Facility_ .JYA NUCLEAR PLANT _____ _ LocatiO.!l_ _!:!AMIL TO..!::!_ COUNTY __________ _ c J 101 G I c=:e_l=RMIT NUMBER [[.2llicHAR-GE NUMBER I r--MONITORING PERIOD I 1 YEAR L!V!o 1 DAY J [YEAR 1 Mo=r:DAu DIFFUSER DISCHARGE EFFLUENT ATTN: Stephanie A. Howard !TEMPERATURE, WATER DEG. CENTIGRADE 1 00010 z 0 0 INSTREAM MONITORING ]TEMPERATURE, WATER DEG. !CENTIGRADE looo10 1 o o lgFLUENT GROSS VALUE 1TEMP. DIFF. BETWEEN SJ! IUPSTRM DEG.C :ooo16 1 s o GROSS VALUE IPH 00400 0 0 EFFLUENT GROSS VALUE !SOLIDS, TOTAL SUSPENDED 1 0 0 jEFFLUENT GROSS VALUE lOlL AND GREASE i 100556 1 0 0 I EFFLUENT GROSS VALUE I W, IN CONDUIT OR THRU ATMENT PLANT i50050 1 0 0 !EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT ..

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RCORDR uous I NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that thiS document and all attachments were prepared under my Jlliphu_rLUL. CA. TELEPHONE I DATE 423 843-6700 1 091 07 r4 I direction or superv1s1on 1n accordance w1th a system des1gned to assure that qualtf1ed personnel Timothy P. Cleary properly gather and evaluate the informat1on submttted Based on my 1nquiry of the person or persons who manage the system, or those persons d1rectly respons1ble for gathering the Principal Environmental Engineer 1nformat1on, the informatmn subm1tted 1s , to the best of my knowledge and beltef, true, I Sequoyah Site Vice President accurate, and complete. I am aware that there are S1gn1f1cant penalties for submitting false I SIGNATURE OF PRINCIPAL EXECUTIVE AREA ---NUMBER-F informat1on, including the possibility of f1ne and 1mpnsonment for knowing violat1ons OFFICER OR AUTHORIZED AGENT L_ TYPED OR PRINTED CODE I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2 DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 06/10/2009 @ 1112 0.16 mg/1 06/15/2009 @ 1112 JOB EPH CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 06/10/2009 @ 1110 <0.10 mg/1 06/15/2009 @ 1123 JOB EPH PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L _.E. ___________ _ -__ _§_ODDY-DAISY _ _JN 3738i_ ______ _ Facilitv _ _IYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l_ .J::!AMIL TOJi.COUNT:L_ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMRJ PERMIT NUMBER 101 G [Dis-CHARGE NUMBER --MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT *** NO DISCHARGE CJ *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form I PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY' OF ANALYSIS T. 1150060 1 0 0 EFFLUENT GROSS VALUE TEMPERATURE-C, RATE OF CHANGE 82234 0 0 jEFFLUENT GROSS VALUE I .,, PERMI'F;'

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' ' '" ' i,-I NAMErTITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary I Certify under penalty of law that th1s document and all attachments were prepared under my direction or supervision 1n accordance w1th a system designed to assure that qual1f1ed personnel properly gather and evaluate the informat1on submitted. Based on my 1nqu1ry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true. I I __ Sequoyah Site Vice President TYPED OR PRINTED accurate, and complete. I am aware that there are sign1f1cant penalt1es for submitting false informat1on, 1ncludmg the poss1bility of fme and Imprisonment for know1ng violations COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Jtc '* I nVrt:'\ JJliL* !-+' l'.f*' >r*1 , lL"**A'\..* , \.,..... .* Y Principal Environmental Engineer r--siGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT TELEPHONE DATE I I 423 843-6700 09 07 I 14 I AREA I NUMBER I YEAR I MO I DAY] CODE The following injections occured: 1. Biodetergent 73551 (max. calc. cone. was 0.019mg/L--Iimit 2.0mg/L) 2. MSW-1 01 (max. calc. cone. was 0.077mg/L-Iimit 0.2mg/L) 3. H-150M (max. calc. cone. was 0.039mg/L--Iimii 0.050mg/L)

4. H-150M (low detection level analytical method was <0.050mg/L--Iimit 0.050mg/L)

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 2 of 2 PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004

  • -__ _i!.NTEROFFICE

_______ _ _ _ _ __§_ODD..1_- DAISY _ _.It'!.]7381_ ______ _ DIFFUSER DISCHARGE Facility _ _I:./ A -_§.!'QUOYAH NUCLEAR PLANT _____ _ Locatio.!!_ _!:!AMIL TOJ:!_COUNT1:_ _________ _ EFFLUENT NO DISCHARGE *** From ATTN: Stephanie A. Howard NOTE: Read instructions before completinQ this form. QUANTITY OR LOADING QUALITY oR coNCENTRATION TNo. f EX OF TYPE ' ANALYSIS ******** ******** 19 0 1 I 91 <0.20 PARAMETER 101022 1 0 0 l'. PERMIT I f r*LUENT GROSS VALUE

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I I INN VIE/TITLE PRINCIPAL EXECUTIVE OFFICER i I I c Timothy P. Cleary Sequoyah Site Vice President TYPED OR PRINTED -------** I Cert1fy under penalty of taw that this document and al! attachments were prepared under my direction or supervision 1n accordance with a system des1gned to assure that qual1f1ed personnel properly gather and evaluate the Information submitted. Based on my inqu1ry of the person or persons who manage the system, or those persons directly responsible for gathering the Information, the information submitted is , to the best of my knowledge and belief. true, accurate, and complete. I am aware that there are Significant penalties for submitting false information, including the possibility of f1ne and impnsonment for knowing violations L COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Boron was sampled on 04/08/2009. EPA Form 3320-1 (REV 3/99) Previous editions may be used /!tt.tvt*w*LccQ,5Ucnccud Principal Environmental Engineer --SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE DATE --I i 423 843-6700 -j-\YEAR-MO DAY_ NUMBER AREA CODE Page 1 of 1 . PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT IDMRJ MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Add res.§_ ___________ _ -__ __§_ODD.Y_- DAISY _ _}t!]738i__ ______ _ BIOMONITORING FOR OUTFALL 101 Facility_ JYA -_§E:QUOYAH NUCLEAR PLANT _____ _ Locatio.!l_ _!:!AMIL TO.Ji_COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard NO DISCHARGE CJ *** NOTE: Read instructions before completinq this form PARAMETER QUANTITY OR LOADING T-ION I NO. 1 FREOUEN_c_Y_S_A_M_P_L_E_ EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS I ANALYSIS IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 0 0 EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR I PIMEPHALES ,TRP6C 1 0 0 !EFFLUENT GROSS VALUE ******** ******** ******** ******** I .. NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my direction or supervision 1n accordance Wllh a system des1gned to assure that qual1f1ed personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inqwy of the person or persons who manage the system, or those persons d1rectly responsible for gathenng the . . . 1nformatton. the 1nformat1on submttted is , to the best of my knowledge and bel1ef, true, Sequoyah S1te VIce President accurate, and complete 1 am aware that there are sigmf1cant penalties for subm1tt1ng false ________ _, 1 information, 1ncluding the possibt!1ty of f1ne and tmpnsonment for know1ng violat1ons l____ TYPED OR PRINTED _l_ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in June 2009. EPA Form 3320-1 (REV 3/99) Previous editions may be used . '*' ' C' i \, I /Jr.tphcut,.u: ... 1 Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 23 SEE" rCOMPOS SEE .. ICOMPOS,I DATE j 423---:3-6700 I 1 kEAFJ MO iDAY_1. CODE l_ *-Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res§_ ...£..9c._BOX.1.QOO ___________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT !DMR! MAJOR (SUBR 01) F-FINAL Form Approved OMB No. 2040-0004 -__ 20DD.1_-DAISY _ _IN 3738'!_ ______ _ L TN0026450 _] [== 1 o3 G CPiscHARG-E LOW VOL. WASTE TREATMENT POND Facility _ _IVA -_g:QUOYAH NUCLEAR PLANT _____ _ __i::!AMIL TOl:!_COUNTL _________ _ EFFLUENT *** NO DISCHARGE

      • ATIN: Stephanie A. Howard NOTE: Read instructions before completinq this form. r PARAMETER

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-, , 0 ---i NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that thiS document and all attachments were prepared under my I ' ' ' c**. J I '-'I TELEPHONE=f ,_I . or superv1s1on 1n accordance w1th a system des1gned to assure that quallf1ed personnel Jj r 1 !1\ t. r: 1/' uC t\., UAC \ :T Timothy P. Cleary properly gather and evaluate the 1nforma!ion submitted Based on my InqUiry of the person or ,.>LJ,*"'-4; ¥ Lu... v *'-* -I 1 persons who manage the system or those persons d. respons1ble for gathenng the PrinCipal Environmental Engmeer I 14 1' . . . 1nformat1on the Information subm1tted 1s to the best of my knowledge and bel1ef true =bi423 843-6700 09 07 1 1 Sequoyah Site VICe President accurate. and complete I am aware that there are 51gnif1cant penalties for submitt*ng false SIGNATURE OF PRINCIPAL EXECUTIVE _1__ 1 , oj**** ... 1 1-. ---------*-----

1'nformat1on.

1nclud1ng the possibility of fine and 1mpnsonment for know1ng violat1ons. OFFICER OR AUTHORIZED AGENT AREA 1 NUMBER YEAR I' MO DAY j : TYPED OR PRINTED CODE I I _j l

... _____ .. -COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facililv Name/Location if Different)

___ _ Addres.L_ _E ___________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) DISCHARGE MONITORING REPORT !DMRJ MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 -__ _J?_ODDY-DAISY _ _IN ______ _ TN0026450 I 107 G NUMBER METAL CLEANING WASTE POND Facility_ .JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!}_ _!:!AMIL TOJ::!.COUNTY._ _________ _ EFFLUENT c= MONITORING PERIOD [iEARJ MP "T DAY I @] *** *** NO DISCHARGE ATTN: Stephanie A Howard From Cos I o6I o1l To [ os-:T os I 30] NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. FRE.--:Q_U_E_N-:-CY---r-:-S_A_M_P_L_E---, EX OF TYPE [PH-AVERAGE UNITS MINIMUM AVERAGE UNITS ANALYSIS I SAMPLE ******** ******** ******** I MEASUREMENT I ** 12 00400 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED i PE,RMiT *** " RECiUIJ:{EMENI SAMPLE MEASUREMENT

                • 1 00530 1 0 0 GROSS VALUE lOlL AND GREASE I ....

ioo556 1 o o !EFFLUENT GROSS VALUE SAMPLE MEASUREMENT

REQI;rlREMENT. I :::r !PHOSPHORUS, TOTAL (AS P) I SAMPLE I MEASUREMENT I 00665 1 0 0

  • PERMIT.*.:*'*

EFFLUENT GROSS VALUE I*

  • COPPER, TOTAL (AS CU) ::>AMPLE MEASUREMENT
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1 0 0 GROSS VALUE 1: ;;_ ******** RON, TOTAL (AS FE) 01045 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT

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  • .. _ I ... 9.0 :; MINIMUM .* < :;,. * ..... *> : .... MAXIMlJM ******** ********
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" .. * ******** ******** 1,Q DAILYMX I ******** ""*"*11 ..... ; l ******** su .DAILY .!**GRAB 19 MG/L 1* *I lcoM;.os 19 1*. ..*. ,:- ' < \";:j;-c, "'o, ;,_:-; MG/L 19 MG/L };OAIL Y !¢OMP.OS

  • 19 MG/L OAIL Y 19 MG/L I l D,t?IL y I COMPOS I . I DAILY lpALCTDl I NAME!TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that.thls doc.u ment and all attachments were prepared un er my I *: '-.... 1. *. . . .. . ('!
  • * .. * \ .. '* .... {. TELEPHONE DATE j *drrect 1 on or superv1s1on 1n accordance wrth a system desrgned to assure that qualrfred ersonnel *Ltf\ j ) ;([*

\_). \.]J(:: ( L.:{ <...'(_,. --T 1 1 . Timothy p. Cleary properly gather and evaluate the 1nformat1on subm1tted. Based on my rnqu1ry of the per on or *'*.f 'kc't' "-' I 1 I persons who manage the system. or those persons d11ectly respons1ble for gathenng th Principal Environmental Engineer 423 843 6700 09 I 07 14 1 ! . . . rnformatron. the informatron submitted rs . to the best of my knowledge and belief. true. -! ! Sequoyah Stte V1ce President accurate. and complete. 1 am aware that there are s1gn1ficant penaltresfor subm1tling fa se l SIGNATURE OF PRINCIPAL EXECUTIVE I ' f.--**----Information. Including the poss1b111ty of frne and rmpnsonment for knowrng vrolatrons OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR I MO [DAY L__ TYPED OR PRINTED CODE _ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L ___________ _ -__ _§ODD..Y_- DAISY _ _IN 37381_ ______ _ Facilitv _ _IVA -..§EQUOYAH NUCLEAR PLANT _____ _ Locatio.!!_ J::!AMIL TOJi.COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I ... Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. I PARAMETER I !TEMPERATURE, WATER DEG. EX I OF QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. I FREQUENCY AVERAGE MAXIMUM MINIMUM AVERAGE MAXIMUM ANALYSIS SAMPLE TYPE 100010 z 0 0 MONITORING IPH i 00400 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT PERMIT .. REO!.IIRJ;:MENT -'{-FoJr-SAMPLE MEASUREMENT PERMIT";:. 'I

  • * .. SAMPLE MEASUREMENT
                • ******** ******** ******** ******** ******** 1 oo53o 1 o o ,*,*?i,::lfERMrr
  • IEFFLUENT GROSS VALUE
  • '"' . iOIL AND GREASE SAMPLE> ******** I MEASUREMENT loo556 1 o o !EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU SAMPLE MEASUREMENT
                • !TREATMENT PLANT 5oo5o 1 o o . ,

.. . . .. REPQR1:H ,EFFLUENT GRoss vALUE bArt:v Mi !CHLORINE, TOTAL RESIDUAL SAMPLE I MEASUREMENT GR 0 oss 0 vALUE F SAMPLE I MEASUREMENT

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  • l -I Certify under penalty of law that this document and all attachments were prepared under my I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER direction or superv1sion in accordance w1th a system designed to assure that qualified personnel I Timothy P. Cleary properly gather and evaluate the informat1on submitted.

Based on my inquiry of the person or i persons who manage the system, or those persons d1rect!y responsible for gathenng the I information, the tnformation subm1tted 1s , to the best of my knowledge and bel1ef, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are s1gn1ficant penalt1es for subm1tt1ng false information, tncludmg the possibility of ftne and 1mpnsonment for vioiatJOns L TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Penod EPA Form 3320-1 (REV 3/99) Previous editions may be used Principal Environmental Engineer I SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE DATE j 09 I 423 843-6700 07 14 AREA NUMBER YEAR I MO DAY I CODE ! Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ ___ ..§.ODD...::L-DAISY _ _IN 37381._ ______ _ Facilitv_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio!!_ __!:!AMIL TO.!!_ COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE

      • Form Approved.

OMB No. 2040-0004 ATTN: Stephanie A. Howard NOTE: Read instructions before completinq this form. , PARAMETER x* QUANTITY OR LOADING -QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE l I EX OF TYPE I I AVERAGE I MAXIMUM I UNITS MINIMUM I AVERAGE I MAXIMUM I UNITS ANALYSIS IIC25 STATRE 7DAY CHR SAMPLE ******** I ******** I ** I I ******** I ******** I I ---l CERIODAPHNIA .. c A"' !DC .. C. IT 23 TRP3B 1 0 0 !EFFLUENT GROSS VALUE ilc25 STATRE 7DAY CHR JPIMEPHALES I TRP6C 1 0 0 EFFLUENT GROSS VALUE h - I , .1 I I I I ,.. . I I I I I l NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that thiS document and all attachments were prepared under my I I I I

  • c* rl I TELEPHONE DATE I d1rect1on or superviSIOn 1n accordance w1th a system des1gned to assure that qual1f1ed personnel A+..: !'\.) , t1 i1 -") :\, \ 1*1 (i l_,<._)l L lC ---1 Timothy p. Cleary properly gather and evaluate the 1nformat1on submitted Based on my tnqUiry of the person or I N1J:...>+IV

\1.-t-\..A ... L * "' I I persons who manage the system or those persons responsible for gathenng the Pnnc 1 pal Environmental Engmeer I I 14 , . . . tnformatton the 1nformat1on subm1tted 1s to the best of my knowledge and belief true ,_____________________ 423 843-6700 09 I 07 1 Sequoyah Site V1ce President accurate, and complete 1 am aware that there are stgn*ficant penalties for submttttng false J SIGNATURE OF PRINCIPAL EXECUTIVE 1 ----*ry--it' J _______ Information, tnciud1ng the possib1l1ty of fine and 1mpnsonment for know1ng VIOlations OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY 1 _____ TY __ PRINTED ___ __L___ _ I __j COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editiOns may be used Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L ___________ _ -__ __§_ODDY-DAISY _ _IN ______ _ Facility _ _sJA-NUCLEAR PLANT _____ _ Locatlo.!l_ _!:!AMIL TOJ:!..COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) TN0026450 I L_ 116 G J C:RMIT NUMBER ] [DISCHARGE MONITQRLNG PERIOD ] MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard I PARAMETER LiEAR I MO I D_6i] To 06 I 30 l NO DISCHARGE CJ *** QUANTITY OR LOADING I JoEBRIS ******** AVERAGE UNITS MAXIMUM MINIMUM ******** ******** 101345 1 0 0 ,EFFLUENT GROSS VALUE OIL AND GREASE VISUAL 84066 1 0 0 GROSS VALUE NOTE: Read instructions before completinq this form QUALITY OR CONCENTRATION AVERAGE MAXIMUM ******** 0 REPORF:. MO'lOTAl.*

                • UNITS 9A PASS=O FAIL=1 NU. !FREQUENCY!

EX OF TYPE ANALYSIS 0 I ____j 1 I 30 VISUAL 'VISUAL SEE ! VISUAL PERMit 1. IP£RMrr I I I 1 I I . . ' . I I I I I I NAMErTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of !aw that this document and all attachments were prepared under my TELEPHONE DATE ! direct1on or supervision in accordance with a system designed to assure that qualified personnel I Timothy P. Cleary properly gather and evaluate the informat1on submitted. Based on my inquiry of the person or I persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 information, the information subm1tted 1s , to the best of my knowledge and bel1ef, true. Sequoyah Site Vice President I accurate, and complete. I am that there are s1.gn1ficant penalties. for subm1ttmg false SIGNATURE OF PRINCIPAL EXECUTIVE 1---;:;:REA-t-NUMBER I 1 1nformat1on, 1nc!udmg the poss1b111ty of fine and 1mpnsonment for know1ng VIOlations OFFICER OR AUTHORIZED AGENT I TYPED OR PRINTED CODE L__ ________ -* ------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ AddresL _E ___________ _ -__ _!2_0DD_l_- DAISY _ ___JN 3738'!.._ ______ _ Facility _ _IYA -..§!::QUOYAH NUCLEAR PLANT _____ _ Locatio_r:1__ _jjAMIL TOl!_COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) l -- I 117 JLJ [ -pffiMiTNUMBER-] [DISCHARGE [-__ ----NiQNlTQRlli_G_ PERIOD I MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT NO DISCHARGE LJ ... Form Approved. OMB No. 2040-0004 A TIN: Stephanie A. Howard I To I I NOTE: Read instructions before completinq this form. I PARAMETER r:>::: QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX OF TYPE -AVERAGE--MAXIMUM MINIMUM 11\/E.RAGE MAXIMUM UNITS ANALYSIS i !oo300 1 0 0 \EFFLUENT GROSS VALUE 1 SOLIDS, TOTAL SUSPENDED 100530 1 0 0 i EFFLUENT GROSS VALUE !SOLIDS, SETTLEABLE I 1 00545 1 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT

.***h***f-f_;* c ' }, SAMPLE MEASUREMENT

                • SAMPLE ******** MEASUREMENT
    • ' REQUIREMENT

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' %' SAMPLE i MEASUREMENT l_ I . 03 MGD ******** ******** . 2.o*.. **#****

-******** ******** >'>": c ******** ******** 100* DAILYM.X 1 __ .*. ******** ******** 1 19 MG/L "fo/\/ICE/ 'dRAB WE EKe, 19 MG/L I JWICEZ I GRAB WEEK 25 MLIL J I Certify under penalty of law that this documenl and all attachments were prepared under my r:* I ' ' c*.. i \ j'.: TELEPHONE I DATE I direct 1 on or supervision 1.n accordance a system d. es1gned to assure qualified personnel J.;l-,} 'i{ ; ( f 1 \ f i/;./ 'l '\j', f r-\. ! --1 properly gather and evaluate the 1nformat10n subm1tted. Based on my 1nqu1ry of the person or /"<*fll4JY [i.},...V _.._, --\./\ \! .. 1 1 persons who manage the system, or those persons drrectly responsible for gatherrng the Principal Environmental Engineer 23 8 0 1 09 07 14 i 1nformatron. the rnformat1on submitted 1s, to the best of my knowledge and belle( true. . 4 43-670 . . ! Sequoyah S1te V1ce President I accurate, and complete. 1 am aware that there are significant penalt1es for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I __ .. --a=-----k

J;. I ---j1nformat1on, rncludrng the poss1brlrty of frne and rmprrsonment for know1ng V1olat1ons OFFICER OR. AUTHORIZED AGENT _j' AREA NUMBER 1 YEAR MO I DAY 1 I TYPED OR PRINTED

_CODE _l __ j NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P Cleary COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all ;ttachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1}}

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