ML23233A014

Discharge Monitoring Report (Dmr), July 2023

ML23233A014
Person / Time
Site:	Sequoyah
Issue date:	08/15/2023
From:	Markum T Tennessee Valley Authority
To:	Office of Nuclear Reactor Regulation, Document Control Desk
References
Download: ML23233A014 (1)

Text

Tennessee Valley Authority, Sequoyah Nuclear Plant, P.O. Box 2000, Soddy Daisy, TN 37384 August 15, 2023 ATTN: Document Control Desk U.S. Nuclear Regulatory Commission Washington, D.C. 20555-0001

Subject:

Sequoyah Nuclear Plant, Discharge Monitoring Report (DMR), July 2023 Attached is the July 2023 DMR for Sequoyah Nuclear Plant.

Respectfully, Travis R. Markum Environmental Scientist 1\\14 TENNESSEE VALLEY AUTHORITY

DMR Copy of Record Permit Permit #:

TN0026450 Permittee:

Tennessee Valley Authority (TVA)

Facility:

TVA SEQUOYAH NUCLEAR PLANT (SQN)

Major:

Yes Permittee Address:

Sequoyah Access Road, PO Box 2000 Soddy Daisy, TN 37379 Facility Location:

SEQUOYAH ACCESS ROAD SODDY DAISY SODDY DAISY, TN 37379 Permitted Feature:

101 External Outfall Discharge:

101-G (no description)

Report Dates & Status Monitoring Period:

From 07/01/23 to 07/31/23 DMR Due Date:

08/15/23 Status:

NetDMR Validated Considerations for Form Completion Primary discharge from Diffuser Pond.

Principal Executive Officer First Name:

Thomas

Title:

Vice President Telephone:

423-843-7001 Last Name:

Marshall No Data Indicator (NODI)

Form NODI:

Parameter Monitoring Location Season Param.

NODI Quantity or Loading Quality or Concentration

1. of Ex.

Frequency of Analysis Sample Type Code Name Qualifier 1

Value 1 Qualifier 2

Value 2 Units Qualifier 1

Value 1 Qualifier 2

Value 2

Qualifier 3

Value 3 Units 00010 Temperature, water deg. centigrade 1 - Effluent Gross 0

Sample

=

42.8 04 - deg C

99/99 - Continuous RC - Recorder (auto)

Permit Req.

Req Mon DAILY MX 04 - deg C

99/99 - Continuous RC - Recorder (auto)

Value NODI 00010 Temperature, water deg. centigrade Z - Instream Monitoring 0

Sample

=

29.5 04 - deg C

99/99 - Continuous CA - CALCTD Permit Req.

<=

30.5 DAILY MX 04 - deg C

99/99 - Continuous CA - CALCTD Value NODI 00016 Temp. diff. between samp. & upstrm deg. C 1 - Effluent Gross 1

Sample

=

2.3 04 - deg C

99/99 - Continuous CA - CALCTD Permit Req.

<=

3.0 DAILY MX 04 - deg C

99/99 - Continuous CA - CALCTD Value NODI 50050 Flow, in conduit or thru treatment plant 1 - Effluent Gross 0

Sample

=

1758.3

=

1776.5 03 - MGD 99/99 - Continuous RC - Recorder (auto)

Permit Req.

Req Mon MO AVG Req Mon DAILY MX 03 - MGD 99/99 - Continuous RC - Recorder (auto)

Value NODI 50060 Chlorine, total residual 1 - Effluent Gross 0

Sample Permit Req.

<=

0.027 MO AVG

<=

0.047 DAILY MX 19 -

mg/L 01/07 - Weekly GR - GRAB Value NODI B - Below Detection Limit/No Detection B - Below Detection Limit/No Detection 82234 Temperature rate of change deg. C/hr Z - Instream Monitoring 0

Sample

=

1.4 62 - deg C/hr 99/99 - Continuous CA - CALCTD Permit Req.

<=

2.0 DAILY MX 62 - deg C/hr 99/99 - Continuous CA - CALCTD Value NODI TRP3B IC25 Static Renewal 7 Day Chronic Ceriodaphnia dubia 1 - Effluent Gross 0

Sample 100.0 23 - %

01/30 - Monthly CP - COMPOS Permit Req.

69.0 MINIMUM 23 - %

01/30 - Monthly CP - COMPOS Value NODI TRP6C IC25 Static Renewal 7 Day Chronic Chrpimephales 1 - Effluent Gross 0

Sample 100.0 23 - %

01/30 - Monthly CP - COMPOS Permit Req.

69.0 MINIMUM 23 - %

01/30 - Monthly CP - COMPOS Value NODI Submission Note If a parameter row does not contain any values for the Sample nor Effluent Trading, then none of the following fields will be submitted for that row: Units, Number of Excursions, Frequency of Analysis, and Sample Type.

Edit Check Errors

No errors.

Comments Method Detection Limit for Total Residual Chlorine is 0.05 mg/L.

Attachments Name Type Size SQN_WET_Biomonitoring_Report_July_2023.pdf pdf 6326609.0 Report Last Saved By Tennessee Valley Authority (TVA)

User:

TRMARKUM Name:

Travis Markum E-Mail:

trmarkum@tva.gov Date/Time:

2023-08-14 15:19 (Time Zone: -05:00)

Report Last Signed By User:

TMCMUTUA Name:

Tony McMutuary E-Mail:

tmcmutuary@tva.gov Date/Time:

2023-08-15 06:55 (Time Zone: -05:00)

DMR Copy of Record Permit Permit #:

TN0026450 Permittee:

Tennessee Valley Authority (TVA)

Facility:

TVA SEQUOYAH NUCLEAR PLANT (SQN)

Major:

Yes Permittee Address:

Sequoyah Access Road, PO Box 2000 Soddy Daisy, TN 37379 Facility Location:

SEQUOYAH ACCESS ROAD SODDY DAISY SODDY DAISY, TN 37379 Permitted Feature:

103 Internal Outfall Discharge:

103-G (no description)

Report Dates & Status Monitoring Period:

From 07/01/23 to 07/31/23 DMR Due Date:

08/15/23 Status:

NetDMR Validated Considerations for Form Completion Internal Monitoring Point for various flows from Low Volume Waste Treatment Pond to Diffuser Pond, which eventually discharges through Outfall 101.

Principal Executive Officer First Name:

Thomas

Title:

Vice President Telephone:

423-843-7001 Last Name:

Marshall No Data Indicator (NODI)

Form NODI:

Parameter Monitoring Location Season # Param. NODI Quantity or Loading Quality or Concentration

1. of Ex. Frequency of Analysis Sample Type Code Name Qualifier 1 Value 1 Qualifier 2 Value 2 Units Qualifier 1 Value 1 Qualifier 2 Value 2 Qualifier 3 Value 3 Units 00400 pH IM - Internal Monitoring Point 0

Sample

=

7.2

=

7.2 12 - SU 01/30 - Monthly GR - GRAB Permit Req.

>=

6.0 MINIMUM

<=

9.0 MAXIMUM 12 - SU 01/30 - Monthly GR - GRAB Value NODI 00530 Solids, total suspended IM - Internal Monitoring Point 0

Sample

=

13.0

=

13.0 19 - mg/L 01/30 - Monthly GR - GRAB Permit Req.

<=

30.0 MO AVG <=

100.0 DAILY MX 19 - mg/L 01/30 - Monthly GR - GRAB Value NODI 00556 Oil & Grease IM - Internal Monitoring Point 0

Sample 4.9 4.9 19 - mg/L 01/30 - Monthly GR - GRAB Permit Req.

<=

15.0 MO AVG <=

20.0 DAILY MX 19 - mg/L 01/30 - Monthly GR - GRAB Value NODI 50050 Flow, in conduit or thru treatment plant IM - Internal Monitoring Point 0

Sample

=

0.852

=

0.852 03 - MGD 01/30 - Monthly IN - INSTAN Permit Req.

Req Mon MO AVG Req Mon DAILY MX 03 - MGD 01/30 - Monthly IN - INSTAN Value NODI Submission Note If a parameter row does not contain any values for the Sample nor Effluent Trading, then none of the following fields will be submitted for that row: Units, Number of Excursions, Frequency of Analysis, and Sample Type.

Edit Check Errors No errors.

Comments Attachments No attachments.

Report Last Saved By Tennessee Valley Authority (TVA)

User:

TRMARKUM Name:

Travis Markum E-Mail:

trmarkum@tva.gov Date/Time:

2023-08-14 14:58 (Time Zone: -05:00)

Report Last Signed By User:

TMCMUTUA Name:

Tony McMutuary E-Mail:

tmcmutuary@tva.gov Date/Time:

2023-08-15 06:55 (Time Zone: -05:00) r I

DMR Copy of Record Permit Permit #:

TN0026450 Permittee:

Tennessee Valley Authority (TVA)

Facility:

TVA SEQUOYAH NUCLEAR PLANT (SQN)

Major:

Yes Permittee Address:

Sequoyah Access Road, PO Box 2000 Soddy Daisy, TN 37379 Facility Location:

SEQUOYAH ACCESS ROAD SODDY DAISY SODDY DAISY, TN 37379 Permitted Feature:

107 Internal Outfall Discharge:

107-G (no description)

Report Dates & Status Monitoring Period:

From 07/01/23 to 07/31/23 DMR Due Date:

08/15/23 Status:

NetDMR Validated Considerations for Form Completion Metal Cleaning Waste Pond discharge. No monitoring required for stormwater decanting. Daily monitoring required only during dewatering events.

Principal Executive Officer First Name:

Thomas

Title:

Vice President Telephone:

423-843-7001 Last Name:

Marshall No Data Indicator (NODI)

Form NODI:

Parameter Monitoring Location Season # Param. NODI Quantity or Loading Quality or Concentration

1. of Ex. Frequency of Analysis Sample Type Code Name Qualifier 1 Value 1 Qualifier 2 Value 2 Units Qualifier 1 Value 1 Qualifier 2 Value 2 Qualifier 3 Value 3 Units 00400 pH IM - Internal Monitoring Point 0

Sample Permit Req.

>=

6.0 MINIMUM

<=

9.0 MAXIMUM 12 - SU 01/01 - Daily GR - GRAB Value NODI C - No Discharge C - No Discharge 00530 Solids, total suspended IM - Internal Monitoring Point 0

Sample Permit Req.

<=

30.0 MO AVG

<=

100.0 DAILY MX 19 - mg/L 01/01 - Daily CP - COMPOS Value NODI C - No Discharge C - No Discharge 00556 Oil & Grease IM - Internal Monitoring Point 0

Sample Permit Req.

<=

15.0 MO AVG

<=

20.0 DAILY MX 19 - mg/L 01/01 - Daily GR - GRAB Value NODI C - No Discharge C - No Discharge 01042 Copper, total [as Cu]

IM - Internal Monitoring Point 0

Sample Permit Req.

<=

1.0 MO AVG

<=

1.0 DAILY MX 19 - mg/L 01/01 - Daily CP - COMPOS Value NODI C - No Discharge C - No Discharge 01045 Iron, total [as Fe]

IM - Internal Monitoring Point 0

Sample Permit Req.

<=

1.0 MO AVG

<=

1.0 DAILY MX 19 - mg/L 01/01 - Daily CP - COMPOS Value NODI C - No Discharge C - No Discharge Submission Note If a parameter row does not contain any values for the Sample nor Effluent Trading, then none of the following fields will be submitted for that row: Units, Number of Excursions, Frequency of Analysis, and Sample Type.

Edit Check Errors No errors.

Comments Attachments No attachments.

Report Last Saved By Tennessee Valley Authority (TVA)

User:

TRMARKUM Name:

Travis Markum E-Mail:

trmarkum@tva.gov Date/Time:

2023-08-14 14:51 (Time Zone: -05:00)

Report Last Signed By User:

TMCMUTUA Name:

Tony McMutuary E-Mail:

tmcmutuary@tva.gov Date/Time:

2023-08-15 06:55 (Time Zone: -05:00) r I

DMR Copy of Record Permit Permit #:

TN0026450 Permittee:

Tennessee Valley Authority (TVA)

Facility:

TVA SEQUOYAH NUCLEAR PLANT (SQN)

Major:

Yes Permittee Address:

Sequoyah Access Road, PO Box 2000 Soddy Daisy, TN 37379 Facility Location:

SEQUOYAH ACCESS ROAD SODDY DAISY SODDY DAISY, TN 37379 Permitted Feature:

110 External Outfall Discharge:

110-G (no description)

Report Dates & Status Monitoring Period:

From 07/01/23 to 07/31/23 DMR Due Date:

08/15/23 Status:

NetDMR Validated Considerations for Form Completion Outfall 110 is closed. Only active in the event the plant goes into closed mode.

Principal Executive Officer First Name:

Thomas

Title:

Vice President Telephone:

423-843-7001 Last Name:

Marshall No Data Indicator (NODI)

Form NODI:

Parameter Monitoring Location Season Param.

NODI Quantity or Loading Quality or Concentration

1. of Ex.

Frequency of Analysis Sample Type Code Name Qualifier 1

Value 1 Qualifier 2

Value 2 Units Qualifier 1

Value 1 Qualifier 2

Value 2

Qualifier 3

Value 3 Units 00010 Temperature, water deg. centigrade 1 - Effluent Gross 0

Sample Permit Req.

Req Mon DAILY MX 04 - deg C

99/99 - Continuous CA - CALCTD Value NODI C - No Discharge 00010 Temperature, water deg. centigrade Z - Instream Monitoring 0

Sample Permit Req.

<=

30.5 DAILY MX 04 - deg C 99/99 - Continuous CA - CALCTD Value NODI C - No Discharge 00016 Temp. diff. between samp. & upstrm deg. C 1 - Effluent Gross 1

Sample Permit Req.

<=

3.0 DAILY MX 04 - deg C 99/99 - Continuous CA - CALCTD Value NODI C - No Discharge 50050 Flow, in conduit or thru treatment plant 1 - Effluent Gross 0

Sample Permit Req.

Req Mon MO AVG Req Mon DAILY MX 03 - MGD 99/99 - Continuous RC - Recorder (auto)

Value NODI C - No Discharge C - No Discharge 50060 Chlorine, total residual 1 - Effluent Gross 0

Sample Permit Req.

<=

0.027 MO AVG

<=

0.047 DAILY MX 19 - mg/L 05/WK - Five Per Week GR - GRAB Value NODI C - No Discharge C - No Discharge 82234 Temperature rate of change deg. C/hr Z - Instream Monitoring 0

Sample Permit Req.

<=

30.5 DAILY MX 62 - deg C/hr 99/99 - Continuous CA - CALCTD Value NODI C - No Discharge TRP3B IC25 Static Renewal 7 Day Chronic Ceriodaphnia dubia 1 - Effluent Gross 0

Sample Permit Req.

>=

69.0 MINIMUM 23 - %

01/30 - Monthly GR - GRAB Value NODI C - No Discharge TRP6C IC25 Static Renewal 7 Day Chronic Chrpimephales 1 - Effluent Gross 0

Sample Permit Req.

>=

69.0 MINIMUM 23 - %

01/30 - Monthly GR - GRAB Value NODI C - No Discharge Submission Note If a parameter row does not contain any values for the Sample nor Effluent Trading, then none of the following fields will be submitted for that row: Units, Number of Excursions, Frequency of Analysis, and Sample Type.

Edit Check Errors

No errors.

Comments Attachments No attachments.

Report Last Saved By Tennessee Valley Authority (TVA)

User:

TRMARKUM Name:

Travis Markum E-Mail:

trmarkum@tva.gov Date/Time:

2023-08-14 14:55 (Time Zone: -05:00)

Report Last Signed By User:

TMCMUTUA Name:

Tony McMutuary E-Mail:

tmcmutuary@tva.gov Date/Time:

2023-08-15 06:55 (Time Zone: -05:00)

DMR Copy of Record Permit Permit #:

TN0026450 Permittee:

Tennessee Valley Authority (TVA)

Facility:

TVA SEQUOYAH NUCLEAR PLANT (SQN)

Major:

Yes Permittee Address:

Sequoyah Access Road, PO Box 2000 Soddy Daisy, TN 37379 Facility Location:

SEQUOYAH ACCESS ROAD SODDY DAISY SODDY DAISY, TN 37379 Permitted Feature:

118 External Outfall Discharge:

118-G (no description)

Report Dates & Status Monitoring Period:

From 07/01/23 to 07/31/23 DMR Due Date:

08/15/23 Status:

NetDMR Validated Considerations for Form Completion Principal Executive Officer First Name:

Thomas

Title:

Vice President Telephone:

423-843-7001 Last Name:

Marshall No Data Indicator (NODI)

Form NODI:

Parameter Monitoring Location Season # Param. NODI Quantity or Loading Quality or Concentration

1. of Ex.

Frequency of Analysis Sample Type Code Name Qualifier 1 Value 1 Qualifier 2 Value 2 Units Qualifier 1 Value 1 Qualifier 2 Value 2 Qualifier 3 Value 3 Units 00300 Oxygen, dissolved [DO]

1 - Effluent Gross 0

Sample Permit Req.

>=

2.0 MINIMUM 19 - mg/L 02/07 - Twice Every Week GR - GRAB Value NODI C - No Discharge 00530 Solids, total suspended 1 - Effluent Gross 0

Sample Permit Req.

<=

100.0 DAILY MX 19 - mg/L 02/07 - Twice Every Week GR - GRAB Value NODI C - No Discharge 00545 Solids, settleable 1 - Effluent Gross 0

Sample Permit Req.

<=

1.0 DAILY MX 25 - mL/L 01/30 - Monthly GR - GRAB Value NODI C - No Discharge 50050 Flow, in conduit or thru treatment plant 1 - Effluent Gross 0

Sample Permit Req.

Req Mon MO AVG Req Mon DAILY MX 03 - MGD 01/BA - Once Per Batch ES - ESTIMA Value NODI C - No Discharge C - No Discharge Submission Note If a parameter row does not contain any values for the Sample nor Effluent Trading, then none of the following fields will be submitted for that row: Units, Number of Excursions, Frequency of Analysis, and Sample Type.

Edit Check Errors No errors.

Comments Attachments No attachments.

Report Last Saved By Tennessee Valley Authority (TVA)

User:

TRMARKUM Name:

Travis Markum E-Mail:

trmarkum@tva.gov Date/Time:

2023-08-14 14:56 (Time Zone: -05:00)

Report Last Signed By User:

TMCMUTUA Name:

Tony McMutuary E-Mail:

tmcmutuary@tva.gov Date/Time:

2023-08-15 06:55 (Time Zone: -05:00) r

Page 1 of 90 TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION/ EXECUTIVE

SUMMARY

Report Date: July 31. 2023

1. Facility/ Discharger: Sequoyah Nuclear Plant/ TV A

2. County/ State: Hamilton/ Tennessee

3. NPDES Pennit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1,579

6. Receiving Stream: Tennessee River (TRM 483.6)

7. lQl0: 2,456

8. Outfall Tested: 101

9. Dates Sampled: July 09 - 14. 2023

10. Average Flow on Days Sampled (MGD): 1754.651, 1756.935. 1760.914

11. Pertinent Site Conditions: Production/ operation data will be provided upon request.

12. Test Dates: July 11 - 18, 2023

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested(%):

Pimephales promelas: UV treated Outfall 101: 17.25, 34.5. 69, 84.S. 100 UV treated Intake: l 00 Ceriodaphnia dubia: Non-treated Outfall 101: 17.25, 34.5, 69, 84,5, 100 Non-treated Intake: 100

16. Permit Limit Endpoint (% ): Outfall l O l: IC2s = 69%

l 7. Test Results: Outfall 101: Pimephales pramelas: IC15 > 100%

Ceriodaphnia dubia: IC,5 > l 00%

Page 2 of 90

18. Facility

Contact:

Travis Markum Phone#: (865) 748-3294

19. Consulting/ Testing Lab: Environmental Testing Solutions. Inc.

20. Lab Contact Jim Sumner Phone #: (828) 350-9364

21. TV A

Contact:

Rick Sherrard Phone#: (423) 987-2250

22. Notes: Exposures to samples collected July 09 - 14, 2023 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC2s values, for both species, were > l 00 percent. Exposure of minnows and daphnids to intake samples resulted in no significant differences from the controls during this study period.

Page 3 of 90 METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 10 l, Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY)

(MM-DD-YY)

Arrival Initial (MM-DD-YY)

Sample Time (ET)

Time (ET)

Temp.

TRC*

Time (ET)

ID Collected Received (aC)

(mg/L)

Last Used By 101 07-09-23 / 0700 to 07-10-23 I 1322 2.7

<0.10 07-11-23 / 0806 07-10-23 / 0600 07-12-23 I 0757 Intake 07-09-23 I 0700 to 07-10-23 / 1322 1.9

<O. lO 07-l 1-23 / 0806 07-10-23 I 0600 07-12-23 / 0757 101 07-11-23 / 0700 to 07-12-23 / 1218 1.7

<0.10 07-13-23 I 0805 07-12-23 I 0600 07-14-23 I 0747 Intake 07-11-23 / 0700 to 07-12-23 / 1218 1.3

<0.10 07-13-23 I 0805 07-12-23 I 0600 07-14-23 / 0747 07-13-23 / 0700 to 07-15-23 / 1226tt 101 07-14-23 I 0600 07-15-23 I 1012

2. L l.81

<0.10 07-16-23 I 0834 07-1 7-23 I 073 7 07-13-23 I 0700 to 07-15-23 / 1226tt Intake 07-14-23 I 0600 07-15-23 / 1012 2.3

<0.10 07-16-23 /0834 07-17-23 /0737 "TRC== Total Residual Chlorine tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

ttDue to a courier delay, tests were not renewed within+/- 2 h.ours of test initiation.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a wann water bath.

Aliquots of Outfall 10 l and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Page 4 of 90 Pimephales prome[as Test Organisms:

L Source:

In-house Cultures

2. Age:

Test Method Summary:

I. Test Conditions; Static. Renewal

2. Test Duration:

7 days

3. Control/ Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

1

5. Organisms per Replicate: lQ

6. Test Initiation: (Dateffime): 07-11-23 0726 ET

7. Test Termination: (Dateffime ): 07-18-23 0629 ET

8. Test Temperature: Outfall 101:

Mean= 24.7°C f24.3 - 25.0°C)

9. Physical/ Chemical Ceriodaphnia dubia In-house Cultures Static, Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic 10 l

07-11-23 0806 ET 07-18-23 0713 ET Mean= 24.9°C (24.7-25.2°C)

Measurements:

Alkalinity. hardness, total residual chlorine. and conductivity were measwed at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software {Tidepool Scientific Software. McKinneyville, CA}.

Page 5 of 90 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

l.

Results of a Pimepha/es promelas Chronic/ 7-day Toxicity Test.

(Genus species)

(Type/ Duration)

Conducted July 11 - 18. 2023 using effluent from Outfall 101.

Test Percent Surviving Solutions (time interval used - davs)

(% Effluent)

I 2

3 4

5 6

7

Control, 100 100 100 100 100 100 100 UV-treated 17.25%

100 100 100 100 100 100 100 34.5%

100 100 100 100 100 100 100 69%

100 100 100 100 100 100 100 84.5%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 JOO

Control, 100 100 100 100 100 100 100 Non-treated Test Solutions Mean Dry Weight (mg)

(replicate number)

(% Effluent) 1 2

3 -

4 Mean

Control, 0.659 0.890 0.717 0.756 0.756 UV-treated 17.25%

0.779 0.800 0.829 0.716 0.781 34.5%

0.811 0.780 0.656 0.716 0.741 69%

0.719 0.784 0.732 0.832 0.767 84.5%

0.709 0.783 0.705 0.771 0.742 100.0%

0.708 0.724 0.726 0.811 0.742 Intake 0.808 0.728 0.747 0.678 0.740

Control, 0.849 0.817 0.793 0.800 0.815 Non-treated IC2s Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 69%

Permit Limit: 1.4 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

• TU a= 100/LCso: TUc = 100/ IC2s

Page 6 of 90 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7~day Toxicity Test.

(Genus species)

(Type I Duration)

Conducted July 11 - 18, 2023 using effluent from Outfall IO 1.

Percent Surviving Test (time interval used - days)

Solutions l

2 3

4 5

6 7

(% Effluent)

Control 100 100 100 100 100 100 100 17.25%

100 100 100 100 100 100 100 34.5%

100 100 100 100 100 100 100 69%

100 100 100 100 100 100 100 84.5%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 JOO 100 Test Solutions Reproduction (#young/female/7 days)

(% Effluent)

Data (replicate number) 1 2

3 4

5 6

7 8

9 10 Mean Control 29 29 32 29 27 31 28 27 29 30 29.1 17.25%

33 30 32 29 35 33 36 34 29 34 32.5 34.5%

30 33 36 30 33 36 33 33 34 34 33.2 69%

31 32 36 36 38 35 31 33 32 33 33.7 84.5%

"?

J_

35 35 35 37 36 37 35 34 36 35.2 100.0%

36 35 34 31 33 33 31 35 33 33 33.4 IC2s Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 69%

Permit Limit: 1.4 TUc 95% Confidence Limits:

Upper Limit NA Lower Limit: NA

• TU a= 100/LCso: TUc = 100/ IC2s

Page 7 of 90 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-dav Toxicity Test.

(Genus species)

(Type/ Duration)

Conducted July 11 - 18. 2023 using water from Intake Percent Surviving Test (time interval used - days)

Solutions 1

2 3

4 5

6 7

(% Effluent)

Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 too Test Solutions Reproduction (#young/female/7 days)

(% Effluent)

Data (replicate number) 1 2

3 4

5 6

7 8

9 10 Mean Control 27 30 32 30 31 30 33 32 30 32 30.7 Intake 34 32 32 33 35 38 36 33 35 37 34.5 IC2s Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: NIA Permit Limit: NIA 95% Confidence Limits:

Upper Limit NA Lower Limit NA

• TU a= I 00/LC50: TUc = I 00/ IC2s REFERENCE TOXICANT TEST RES UL TS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC2s)

Pimephales promelas July 11 - 18, 2023 0714 7 days KC!

0.74 g/L Ceriodaphnia dubia July 11 -18, 2023 0756 7 days NaCl I.IO g/L

Page 8 of 90

.

• PHYSICAL/CHEMICAL

SUMMARY

Wal~r Chemistry Mean Values and Ranges for UV-ln:aled Pimephalo1s pramelus and Non-trcmcd Ceriudaphma dubia. Scquoyllh Nuclear Plant (SQN), Effluent Outfall l OJ snd Intake perfonned July \\ J. 18. 2023.

Test Sample ID Temncratun: /°Cl Dissolnd Oxv~cn fm~/U lniti11I Final lnitiltl Final C.cmlrol, 24.7 24.5 7,9 7.1 Non-lK'al11J 2-1.7 -

24.8 N.4

• 24.7 7.7 -

80 6 3.

7.8

(.'ourrol, 24.7 24.5 7.8 7.3 UV~trut,:,d 2n. 24 s 24 3

• 24.7 7.6 8.0 59 -

8.l 17.25%

24.8 24.5 7.8 7.2 14.7 -

24.9 14.3 - 247 7.7 -

8.0 6.0. k.l

~

3-1.~%

24.8 24.5 7.8 7.2

24. 7

• 24.9 24.3

• 24 7 7.7 8.U

.5 8.

8. l

~

24.9 24.6 7.9 7.1 69%

24.8 -

24.9 24.4 -

24.8 7.7

• 8.0 5.8.

8.0 t

U.5%

24.9 24.5 7.9 7.1 ii:

24.8 -

25 0 24.4

• 24 8 7.7

• 8 I 5 8 8.l l00%

25.0 24.5 7.9 7.2 25.0 -

25.0 24.4

• 24.6 1.5.

8.1 5.9.

8.1 lnhlk~

25.0 24.S 7.CJ 7 I 24 8 -

25.0 24.4

• 2H 1.6. 8.2 H

8.1 C'adlt111I, 24.8 25 0 7.9 7.9 N1kHk'tcd 24 7

• 24 9 248

• 25 2 7.7 -

80 7.7 -

8.0 17.25%

24.8 25.0 7.9 7.9

.S; 2.4.7 -

24.9 24.9 -

25 2 7.6 81J 7.7.

8.0

~

24.S 25.0 7.9 7.9 J.J.5%

24 7

• 25.0 24.7

• 25 2 7.6 8.U 78 8.0

'i:

69%

24.9 25.0 7.9 7.9 2 250 24.7 -

25 2 7.6 -

8.0 7.8 8.0 f

-~

114.5%

24.9 24.9 80 7 9

~

24 8

• 25 o 24.7

• 25 I 7.7 8 l 7.7. 8.l 100%

25.0 24.9 so so 14.9 -

25.ll 2U

• 25.0 76 8.2 77 -

8.1 25.0 25.0 8.0 7.9 Intake 24 9

• 2S.U 24.8 -

25.1 76 8,2 77.

8.1

• Nole T01alrc,idl<llchlorioc was perform,d on oon-trealed OUllilll I Ol ard lnui~e ~ampk:s o,,crall tempualurt'. (

0C)

Pimephales promellu Ceriadaphnia duh,a i\\vcragc 24,7 24,9 Minimum Maximum 24.3 25.0

24. 7 25.2 oH IS.LI. I Initial i;'inal 7.85 7.69 7 73 -

7.99 7.57

• 78!

7.92 7.74 7.84 -

S.02 7 62

• 7.87 7.9!

7.7!

7.81

• 8.02 7.60

• 7.82 7.8~

7.67 7.72 - 7%

7 ss

• 7.78 7.86 7.64 7.63 -

7.96 7.52

• 7. 76 7.83 7.63 759 -

7.91 757

• 7.74 7.82 7.61 7 53 -

7.91 7.48 -

7 73 7.85 7.67 7.62

• 7.96 749

• 7.78 7.85 7-91 7 73 -

7.99 7.86

• 798 7.9!

7.90 7.8b

• 799 7 86

• 7.98 7.90 7.89 7.HO

• 798 785

• 7.95 7.86 7-88 7.64 - 796 7.82

• 7 95 7.81 7_86 7.56. - 793 7 81

• 7.92 7.79 7.85 7.49 - 790 7.80 - 790 7.84 7.89 7.58 -

7.97 7.8J -

7.95 Conductance AlkaUnily

Hatdnes,

• Tolal Resitlm1I (J&mhos/cm) (mg/L CaC01) (mg/L C11C01)

Chlorine (mg/L) 312 6!

86 291 -

325 61 62 84 -

88 JI0 60 85 293

• 327 59 61

&4 -

86 286

17) -

301 260 WI -

272 207

!98 -

220 182 170

• 194 155 58 61

< 0.10

!40

• 164 57.

61 57 65

, 0.10

< 0.10 16ri 66 fl8

<O 10 153

• 174 6S 67 65 1\\

,-o I{}

, 0 lO 312 61 86 291 -

325 ol -

62 84 88 286 275

• 296 260 250

• 273 206 197

• 215 181 173

• 19) 153 58 63

< 0.10

!44 -

163 51.

61 57 69

< 0 IO

< 0. 10 166 66 67

<0.10 160 -

176 63 67 6S 69

< 0 IU.

<.U 10

Page 9 of 90

SUMMARY

I CONCLUSIONS Exposures to samples collected July 09 - 14, 2023 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC2s values, for both species, were

> I 00 percent. Exposure of minnows and daphnids to intake samples resulted in no significant differences from the controls during this study period.