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{{#Wiki_filter:S58 090213 801- NPDES CORRESPONDENCE February 1.3, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JANUARY 2009 Enclosed is the January 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN B. A. Wetzel, OPS 4A-SQN T. P. Cleary, OPS 4A-SQN G. R. Signer, WT 6A-K D. E. Pittman, BR 4T-C EDMS, WT CA-K (Enclosure) DMR0901.doc
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- JANUARY DMR ORGANIZATION: Chemistry and Environmental DOCUMENTPREPAREDBY: AnnHurt DATE: 02/11/2009 EDMS TRACKING NO.: S58 090213 801 - NPDES Correspondence CONCURRENCES Name R c Signature - Comment Date v N Ann Hurt X Stephanie A Howard X Alan K Barringer X Debra J Bodine X Beth A. Wetzel X Chris R Church X Timothy P Cleary X Stephanie A. Howard (for signature) X 2-/t3/0 1 /0/) INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 February 13, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORINGREPORT FOR JANUARY 2009 Enclosed is the January 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant. Piease contact me at (423) 843-6700 if you have any questions or comments. Sincerely, 1 J J I+* *1cuv.L fJ .(/._Ji?lt.c)tLZ. c /4111Jf"i Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRI OMB No. 2040-0004 (SUBR 01) Add res§_ ...f.~BOX 20QQ_ _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 G F- FINAL ---~TEROFFICESB-~---------- ---~DDY-DAISY_~N3738L ______ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facility_ JYA -.§EQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!!_ .J:!AMIL TOl:!_COUNTY __________ _ MONITORING PERIOD EFFLUENT YEAR I MO DAY I DAY NO DISCHARGE [ I ... ATTN: Stephanie A. Howard From I 09 01 01 To 31 NOTE: Read instructions before completinq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. IFREOUENCYI SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM WATERDEG. ******** ******** .. ******** ******** 10.6 04 0 31 I 31 I MODELD 0
. DIFF. BETWEEN SAMP. &
UPSTRM DEG.C 16 1 w 0 EFFLUENT GROSS VALUE PH 00400 1 0 0 EFFLUENT GROSS VALUE I:SULIU:S. TOTAL SUSPENDED 0 0 0 0 GROSS VALUE 50050 0 0 EFFLUENT GROSS VALUE
~.phu!L{. a.dbJA.)(_Lcd I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law thai this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or II 1
persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 09 02 I 11 I Site Vice President I accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I l ' TYPED OR PRINTED information, includ1ng the possibility of fine and impnsonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I CODE NUMBER~AR 1 MO I DAY1 COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: 1. CCW data 2. veliger monitoring data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2
DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 01/14/2009 @2 1120 0.17mg/l 01/15/2009@ 1918 KLM TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 01/14/2009 @ 1117 <0.10 mg/1 01/15/2009@ 1928 KLM TN EPA 8015
Mean# of NOTES:% Mean# of Water Temp. Water Temp. LOCATION SUB Sample Date % Settlers ("C) Sample Date Asiatic Gravid Asiatic COLLECTED BY ZM/m3 ("C) LOCATION Clams/m3 Clam 1112112008 0 0 12 1111612008 230 12 In plant RCW Dick Adcock 1112412008 31 0 11 1112412008 138 11 In plant RCW Dick Adcock 121412008 0 0 10 1210412008 15 10 In plant RCW Dick Adcock 121812008 0 0 9 1210812008 0 9 In plant RCW Dick Adcock 1211512008 0 0 9 1211512008 0 9 lnplant RCW Dick Adcock 1212212008 0 0 9 1212212008 0 9 In plant RCW Dick Adcock 1212912008 0 0 9 1212912008 0 9 In plant RCW Dick Adcock 0110912008 0 0 8 0110912009 0 8 In plant RCW Dick Adcock 0111612008 0 0 7 0111612009 0 7 In plant RCW Adcock I Bryant 01122/2008 0 0 7 01/2212009 0 7 In plant RCW Adcock I Bryant 01/3012009 0 0 9 0113012008 0 9 lnplant RCW Adcock I Bryant
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Na~-~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMS No. 2040-0004 (SUBR 01) Addres_L ...f.SL_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 G F- FINAL ---~TEROFFICESB-~---------- - _ _ 20DD_!:_- DAISY _ _JN 37381._ _ _ _ _ _ _ _ DIFFUSER DISCHARGE Facilitv_ _IYA -..§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l_ _l:!~IL TOJi.COUNTL _ _ _ _ _ _ _ _ _ _ EFFLUENT ATIN: Stephanie A. Howard YEAR From I 09 I - - I - - I NO DISCHARGE D *** NOTE Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION \ NO. IFREQUENCY\ SAMPLE EX OF I TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS LORINE, TOTAL RESIDUAL ******** ******** ******** 0.014 0.030 0 19 0 0
- C, RATE OF 0 0 EFFLUENT GROSS VALUE
-~1(UliL()_,JJo-~cl NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direction or superv1sion in accordance with a system designed to assure that qualified personnel --
Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted IS , to the best of my knowledge and belief, true, 423 843-6700 09 I 02 11 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I l TYPED OR PRINTED information, including the possibility of fine and Imprisonment for know1ng violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR( MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injection occured: 1. Biodetergent 73551 (max. calc. cone. was 0.025mg/L--Iimit 2.0mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres,L ...f.~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 T F- FINAL ---~TEROFFICESB-~---------- - _ _ _§ODD..Y..: DAISY_ ___IN 373BL _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facilitv_ .JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio~ __!:!AMIL TO.!i.COUNTY __________ _ MONITORING PERIOD EFFLUENT ATTN: Stephanie A. Howard From YEAR 09 To DAY 31 NO DISCHARGE CJ *** NOTE Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. EX IFREQUENCY OF SAMPLE TYPE ANALYSIS AVERAGE MAXIMUM UNITS IMUM STATRE 7DAY CHR ******** ******** RIODAPHNIA 1 0 0 7DAY CHR 0 0
~G-~d NAME!TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel i Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the i !
Principal Environmental Engineer I 09 information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 02 11 Site Vice President accurate, and complete I am aware that there are Significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I I TYPED OR PRINTED information, including the possibility of fine and Imprisonment for knowtng violations. OFFICER OR AUTHORIZED AGENT AREA COOE I NUMBER iYEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in January 2009. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT !DMRJ OMB No. 2040-0004 (SUBR 01) Addres,L ..£ ~BOX_19QQ_ _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 103 G F- FINAL ---~TEROFFICESB-~---------- - _ _ __§_ODD.r_- DAISY _ _JN 373BL _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Facility_ .JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!!_ .J:!AMILTOli_COUNTy_ _ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN: Stephanie A. Howard From YEAR 09 DAY 31 NO DISCHARGE D *** NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION \ NO. (FREQUENCY\ SAMPLE EX ! OF \ TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH ******** ******** 7.0 ******** 8.4 0 12 0 0 IN CONDUIT OR THRU ENT PLANT 1 0 0 EFFLUENT GROSS VALUE JtJ~CLdkr~QLcJ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direct1on or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 02 information, the information submitted IS . to the best of my knowledge and belief, true, 09 11 Site Vice President accurate, and complete I am aware that there are sigmficant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY TYPED OR PRINTED CODE
-- ------- I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Name_~A-SEQUO~~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No 2040-0004 (SUBR 01) Addres_L ...f.~BOX~OO _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 107 G F- FINAL ---~TEROFFICESB-~---------- - _ _ _§_ODD..Y_- DAISY _ _JN 373BL_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER METAL CLEANING WASTE POND Facilitv_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _
- Locatio!J_ _!:!AMILTOl:!_COUNTY._ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT ATTN: Stephanie A. Howard From Y~R I ~~ I ~Atl To ~~Y NO DISCHARGE I XX I ***
NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. \FREQUENCY I SAMPLE EX 1 OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM UNITS
******** ******** ** ******** 12 0 0 TOTAL (AS CU) 01042 1 0 0 EFFLUENT GROSS VALUE IRON, TOTAL (AS FE) 0 0 DUITORTHRU PLANT 0
J~Ct~{~ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel I Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or ! persons who manage the system, or those persons directly responsible for gathering the I Principal Environmental Engineer 423 843-6700 11 I information, the information submitted 1S , to the best of my knowledge and belief, true, 09 02 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false 1 I SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, Including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR[ MO DAY I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES} MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres_L_E~BOX2000 - - - - - - - - - - - - TN0026450 110 G F- FINAL ---~TEROFFJCESB-~---------- ---~ODDY-DAISY_~N373BL ______ _ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facility_ .JYA -..§E.QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio..!l._ _jjAMIL TO.!:!._COUNTY __________ _ MONITORJNG PERIOD EFFLUENT YEAR I YEAR I MO I DAY NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard From I 09 To 09 01 31 NOTE: Read instructions before completinq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. !FREQUENCY! SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS
******** ******** 04 ******** ******** 04 0 0 0 0 GROSS VALUE 0
.Jf4;hw~ &41\)~t_J NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE f
direction or supervision in accordance with a system designed to assure that qualified personnel I Timothy P Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or ! i I persons who manage the system, or those persons directly responsible for gathenng the mformation, the information submitted IS , to the best of my knowledge and belief, true, Principal Environmental Engineer 423 843-6700 09 02 11 Site Vice President I accurate, and complete I am aware that there are s1gnific;ant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREAl I NUMBER YEAR MO DAY* I TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ MAJOR Form Approved Na~-~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres_L _E.~BOX.1.QOO _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 F- FINAL ---~TEROFFICESB*~---------- - _ _ _§.ODDY- DAISY_ _IN 37381_ _ _ _ _ _ _ _ RECYCLED COOLING WATER Facilitv_ _IVA-SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio!l._ .J::!AMILTO.!i._COUNTY __________ _ EFFLUENT NO DISCHARGE I XX I *** ATTN: Stephanie A Howard From NOTE: Read instructions before complettn!l this form PARAMETER QUANTITY OR LOADING QUALITY oR coNCENTRATION (No. 1FREouENcvl SAMPLE-~! I EX I OF , TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS
******** ******** ******** ******** 23 0
EFFLUENT GROSS VALUE ANIIIUAL NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my I TELEPHONE DATE Timothy P. Cleary direction or supervision in accordance with a system designed to assure that qualif1ed personnel properly gather and evaluate the information submitted Based on my inquiry of the person or ,JitphaJt,<L Q.JkKXud i persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 09 02 11 Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are Significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I I information, including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED AREA I NUMBER IYEAR I MO I DAY I CODE i i COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRI OMB No. 2040-0004 (SUBR 01) Addres.L__E.~BOX2000 - - - - - - - - - - - -
---~TEROFFICESB-~---------- TN0026450
! *-*- 116_6_l F- FINAL
_ _ _ _§.ODD_Y._- DAISY_ _TN 3738i_ _ _ _ _ _ _ _ BACKWASH Facility_ JYA -_g:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ LocatiO!!... _t!AMIL TO..!'i._COUNTY __________ _ EFFLUENT YEAR DAY 31 NO DISCHARGE D ... ATTN: Stephanie A. Howard From I 09 I -- I -- I NOTE: Read instructions before completin>J this form. PARAMETER QUALITY OR CONCENTRATION I NO. IFREQUENCY SAMPLE EX OF TYPE ANALYSIS IUI=tsKI::I, FLOATING (SEVERITY) 0 1 I 31 VISUAL 0 0 SEE VISUAL ENT GROSS VALUE PERMIT 0 1 I 31 VISUAL I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER
) [pha_ru~~ 0 .cllnco./cd l I I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE I direction or superv1sion in accordance with a system designed to assure that qualified personnel I Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or I I I I persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer I
information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 02 11 I Site Vice President accurate, and complete. I am aware that there are Significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I I 09 1 i I information, including the possibility of fine and Imprisonment for knowing violat1ons. I TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREAl CODE NUMBER jYEARj MO DAY J COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres_L__E.~BOX2000 - - - - - - - - - - - - ---~TEROFFICESB-~---------- TN0026450 117 G F - FINAL _ _ _ 20DDY-DAISY_~N3738L _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER BACKWASH Facilitv_ .JYA -..§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio!)_ .J:!AMILTOJi.COUNTY._ _ _ _ _ _ _ _ _ _ _ __MQJ\jl_TORING PERIOD EFFLUENT ATIN: Stephanie A. Howard From Y~R j ~~ I ~~Y-j To c;1Y NO DISCHARGE CJ ... NOTE: Read instructions before completinq th1s form PARAMETER QUALITY OR CONCENTRATION I NO. \FREQUENCY SAMPLE EX OF TYPE ANALYSIS MINIMUM AVERAGE MAXIMUM UNITS RIS, FLOATING (SEVERITY) ******** ******** 0 9A 0 1 I 31 I VISUAL 0 SEE VISUAL PERMIT IL AND GREASE VISUAL 1 I 31 VISUAL I 0 0 SEE I VISUAL PERMIT A~~G.i.lwxo~d NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel I Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or II persons who manage the system, or those persons directly respons1ble for gathering the I Principal Environmental Engineer information, the information submitted is . to the best of my knowledge and belief, true, 423 843-6700 o9 1 02 11 Site Vice President accurate, and complete. I am aware that there are S1gnif1cant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I i informat1on, Including the possibility of fine and imprisonment for know1ng violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED AREAl CODE NUMBER ~EARl MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT !DMR) OMB No 2040-0004 (SUBR 01) Addres_L .J:.~BOX.1QOO _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 118 G F- FINAL ---~ODDY-DAISY_~N373BL ______ _ DISCHARGE NUMBER I WASTEWATER & STORM WATER PERMIT NUMBER Facility_ JYA -..§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio!!_ _jjAMIL TOJi_COUNTY__________ _ MONITORING PERIOD EFFLUENT From YEAR 09 To DAY 31 NO DISCHARGE IXXJ *** ATTN: Stephanie A. Howard NOTE: Read instructions before completinq this form. PARAMETER QUALITY OR CONCENTRATION I NO. IFREQUENCY 'I SAMPLE EX OF TYPE ANALYSIS UNITS MINIMUM AVERAGE MAXIMUM UNITS (DO) ******** ******** 19 MG/L TWICE/ GRAB WEEK 19 GRAB DUIT ORTHRU T PLANT 0 0 ESTIMA NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my
)~OJU-L U-~'XJOA-d TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel I Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or I Site Vice President persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Principal Environmental Engineer 423 843-6700 I og 02 II 11 1 accurate, and complete. I am aware that there are significant penallies for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE I I information, including the possibility of fine and Imprisonment for knowing violations.
I DAY I
-~~~~ l IYEAR OFFICER OR AUTHORIZED AGENT NUMBER MO TYPED OR PRINTED I I I J COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
S58 090312 800- NPDES CORRESPONDENCE March 12, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR FEBRUARY 2009 Enclosed is the February 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely, ~:!:J Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN B. A. Wetzel, OPS 4A-SQN T. P. Cleary, OPS 4A-SQN G. R. Signer, WT 6A-K D. E. Pittman, BR 4T-C EDMS, WT CA-K (Enclosure) DMR0902.doc
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- FEBRUARY DMR ORGANIZATION: Chemistry and Environmental DOCUMENTPREPAREDBY: AnnHurt DATE: 03/09/2009 EDMS TRACKING NO.: S58 090312 800- NPDES Correspondence CONCURRENCES Name R c Signature - Comment Date v N Ann Hurt Stephanie A Howard X 3/~ ar X 3}q fo9 Alan K Barringer Debra J Bodine Beth A. Wetzel Chris R Church X Timothy P Cleary X IJM~ (for signature) INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 March 12, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR FEBRUARY 2009 Enclosed is the February 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely, ~.~ Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Add res.§_ _E.~BOX_1900 ___________ _ TN0026450 101 G F- FINAL ---~TEROFFICESB-~---------- - _ _ _§_ODDY- DAISY_ _TN 3738L _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facility_ .JYA -.§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ LocatioD__ __!jAMILTOJ::LCOUNTY __________ _ EFFLUENT YEAR DAY From
*** NO DISCHARGE [J ***
ATTN: Stephanie A. Howard 09 28 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO.IFREOUENCYj SAMPLE EX OF I TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS ' TEMPERATURE, WATER DEG_ CENTIGRADE
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00010 0 0 DEG.C. SEE CK REO. EFFLUENT GROSS VALUE PERMIT TEMP. DIFF. BETWEEN SAMP. & ******** ******** 3.3 04 o 1 28t 28 CALCTD j UPSTRM DEG_C 00016 1 w 0 . CONTIN CALCTD EFFLUENT GROSS VALUE uous PH 4 I 28 I GRAB I i 00400 0 0 su W~£KLY I GRAB< EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 0 I 1 /28 ] GRAB 19 I 00530 0 0 MG/L MONTHL Yl GRAB EFFLUENT GROSS VALUE OIL AND GREASE <6 19 ~1 /28 I GRAB 00556 0 0 .* ;~~~~0 F. ~f':.' MG/L V'IGNTHI;:.YI GRAB 1:: EFFLUENT GROSS VALUE ;.O~Il.Y:*MJf FLOW, IN CONDUIT OR THRU ******** ITREATMENT PLANT 0 u 50050 1 0 0 ~RiiPiJl:T . MGD EFFLUENT GROSS VALUE OA:til:~:IIII.X: ' uous NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that.this doc.ument and a. II attachments w.ere prepare.d under my direction or supervision in .accordance with a system designed to assure that qualified personnel
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- Q u,ur 1./,.._ , . . ,_ * /
l,.c._.)C..LA c::1 TELEPHONE I D.ATE Timothy P. Cleary properly gather and evaluate the 1nformahon subm1tted Based on my *nqu1ry of the person or ! 1 1 Sequoyah Stte Vtce President persons who manage the system,or those persons d~rectly respons*ble for gathenng the information. the mlormation submitted 1s . to the best of my knowledge and belief, true, accurate, and complete. 1am aware that there are significant penalties lor submitting false Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE 4 23 I 84 3-6 7 OO 09 l' 1 03
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1 1 I 10 f----------------------l'nformatJon, including the possibility of fine and imprisonment for know1ng violalJans OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR I MO DAY TYPED OR PRINTED CODE i 1 COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: 1. CCW data 2. Veliger monitoring data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2
DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 02/11/2009@ 1058 0.13 mg/1 02/13/2009 @ 1136 DAP TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 02/11/2009 @ 1100 <0.11 mg/1 02/13/2009 @ 1146 DAP TN EPA 8015
Mean# of NOTES:% Mean# of Water Temp. Water Temp. LOCATION SUB Sample Date % Settlers {"C) Sample Date Asiatic Gravid Asiatic COLLECTED BY ZM/m3 ("C) LOCATION Clams/m3 Clam 1112112008 0 0 12 1111612008 230 12 In plant RCW Dick Adcock 1112412008 31 0 11 1112412008 138 11 lnplant RCW Dick Adcock 121412008 0 0 10 1210412008 15 10 In plant RCW Dick Adcock 121812008 0 0 9 1210812008 0 9 lnplant RCW Dick Adcock 1211512008 0 0 9 1211512008 0 9 In plant RCW Dick Adcock 1212212008 0 0 9 1212212008 0 9 In plant RCW Dick Adcock 12129/2008 0 0 9 12129/2008 0 9 lnplant RCW Dick Adcock 0110912009 0 0 8 0110912009 0 8 In plant RCW Dick Adcock 0111612009 0 0 7 0111612009 0 7 In plant RCW Adcock I Bryant 0112212009 0 0 7 0112212009 0 7 In plant RCW Adcock I Bryant 0113012009 0 0 9 0113012009 0 9 In plant RCW Adcock I Bryant 0210612009 0 0 6 0210612009 0 6 lnplant RCW Adcock I Bryant 0211312009 0 0 11 02/1312009 0 11 In plant RCW Love 0212012009 0 0 9 0212012009 0 9 In plant RCW Bryant 0212612009 0 0 9 0212612009 0 9 In plant RCW Bryant
PERMIITEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Na~-~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) Add res§_ _.E.~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ (SUBR 01) OMS No. 2040-0004
---~TEROFFICESB-~---------- TN0026450 101 G F- FINAL - _ _ __§.ODDY- DAISY_ _JN 37381._ _ _ _ _ _ _ _
Facility_ ..JYA -..§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ PERMIT NUMBER f-:D~I==sc:-:H-:-A:-:R:-G:-E.:...::..,N:...UM....:;;_BE...,R~ Dl FFU SER DISCHARGE Locatio.!l.._ _!:!AMIL TOJi.COUNTY_;_ _ _ _ _ _ _ _ _ _ _ EFFLUENT MONITORING PERIOD YEAR DAY ATIN: Stephanie A Howard From I 09 To 28 *** NO DISCHARGE [J *** NOTE: Read instructions before completinq this form. PARAMETER QUALITY OR CONCENTRATION I NO. )FREOUENCYTSAMPLE-EX i OF : TYPE _ __ MAXIMUM UNITS MINIMUM AVERAGE . ANALYSIS . CHLORINE, TOTAL RESIDUAL
******** ******** ******** 0.016 0.030 I
---+i ---+----_;________ j 0 1 19 I 28 : GRAB 19
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0 0 MG/L WEEK- I CALCTD DAYS RE- C, RATE OF 0 28 I 28 I CALCTD 82234 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my , Timothy P. Cleary direction or supervision in accordance with a system designed to assure lhat qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the
~o.Au.c-. d TELEPHONE I
I DATE i Principal Environmental Engineer i I 10 J Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 03 accurate, and complete. I am aware that there are significant penalties for submitting false 1 09 informatton. includtng the possibility of fine and imprisonment for knowing violations SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREAl CODE NUMBER [YEAR 1 MO j DAY I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injection occured: Biodetergent 73551 (max. calc. cone. was 0.021 mgiL--Iimit 2.0mgiL) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2
PERMITIEE NAME!ApDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres_L_f.~BOX2000 - - - - - - - - - - - - F- FINAL ---~TEROffiCESB-~---------- TN0026450 _ _ _ _§ODDY- DAISY_ _JN 37381._ _ _ _ _ _ _ _ BIOMONITORING FOR OUTFALL 101 Facility_ _IVA -SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.n_ .JjAMIL TOJLCOUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard From NO DISCHARGE CJ *** NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREO EX OF ANALYSIS AVERAGE
******** ******** >100.0 ******** ******** 0 1 I 180 23 PERCENT SEE COMPOS PERMIT 0 1 f 180 i COMPOS 23
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~(l~d NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed lo assure thai qualified personnel Timothy P. Cleary properly gather and evaluate lhe information submilled. Based on my inquiry of lhe person or !
I Sequoyah Site Vice President persons who manage lhe system, or those persons directly responsible for gathering the information, the information submitted is , to lhe best of my knowledge and belief. lrue, accurate, and complete. I am aware that there are significant penalties for submitting false Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE 423 l 843-6700 091 03 1 i 10 I -- -- TYPED OR PRINTED information, ~ncluding lhe possibility of ftne and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA CODE 1 NUMBER YEAR[ I MO I DAY i COMMENTS AND EXP,LANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled February 8-13, 2009. Report is attached EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
March 9, 2009 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, FEBRUARY, 2009 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101, samples collected February 8-13, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were> 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment): Sherrard, R. M., PSC 1X-C Files, ER&TA, CEB IB-M SQN February 2009m
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE
SUMMARY
Report Date: March 9, 2009
- 1. Facility I Discharger: Sequoyah Nuclear Plant I TV A
- 2. County I State: Hamilton I Tennessee
- 3. NPDES Permit#: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MOD): 1,579
- 6. Receiving Stream: Tennessee River (TRM 483.6)
- 7. 1Q10: 3,491
- 8. Outfall Tested: 101
- 9. Dates Sampled: February 8-13, 2009
- 10. Average Flow on Days Sampled (MOD): 1582, 1597, 1596
- 11. Pertinent Site Conditions: Production I operation data will be provided upon request.
- 12. Test Dates: February 10-17, 2009
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia)
- 15. Concentrations Tested(%): Outfall101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephales promelas: UV treated Outfall101: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0
- 16. Permit Limit Endpoint (% ): Outfall 101: ICzs = 45.2%
- 17. Test Results: OutfalllOl: Pimephales promelas: IC25 > 100%
Ceriodaphnia dubia: IC2s > 100% UV treated Outfall101: Pimephales promelas: IC£2 > 100% Page 1 of98
- 18. Facility
Contact:
Ann Hurt Phone#: (423) 843-6714
- 19. Consulting I Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Cynthia L. Russell Phone#: (256) 386-2755
- 22. Notes: OutfalllOl samples collected February 8-13, 2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were>
100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 2 of98
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 10 1, Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. t TRC* Time (ET) ID Collected Received (OC) (mg/L) Last Used By 02-08-09 0921 to 02-10-09 1110 101 02-09-09 1554 1.7, 1.9 <0.10 02-09-09 0821 02-11-09 1206 02-08-09 0940 to 02-10-09 1110 Intake 02-09-09 1554 2.4 <0.10 02-09-09 0840 02-11-09 1206 02-10-09 0915 to 02-12-09 1030 101 02-11-09 1553 2.2, 1.6 <0.10 02-11-09 0815 02-13-09 1110 02-10-09 0936 to 02-12-09 1030 Intake 02-11-09 1553 3.1 <0.10 02-11-09 0836 02-13-09 1110 02-14-09 1012 02-12-09 0936 to 101 02-13-09 1531 1.5, 1.5 <0.10 02-15-09 1014 02-13-09 0836 02-16-09 1011 02-14-09 1012 02-12-09 0842 to Intake 02-13-09 1531 0.7 <0.10 02-15-09 1014 02-13-09 0742 02-16-09 1011
*TRC =Total Residual Chlonne tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV -treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Page 3 of98
Pimephales promelas Ceriodaphnia duhia Test Organisms:
- 1. Source: Aquatox, Inc. In-house Cultures
- 2. Age: 19 to 19.17 hours old <24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control I Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 1
- 5. Organisms per Replicate: 1.Q 1
- 6. Test Initiation: (Date/Time)
Outfall101 02-10-09 1110 ET 02-10-09 0830 ET UV Treated Outfall 101 02-10-09 1100 ET
- 7. Test Termination: (Date/Time)
Outfall 101 02-17-09 1030 ET 02-16-09 0913 ET UV Treated Outfall 101 02-17-09 1046 ET
- 8. Test Temperature: Outfall 101: Mean= 24.6°C Mean= 24.8°C (24.1- 24.9°C} (24.5- 25.1 °C)
Test Temperature: UV-Treated Outfall101: Mean= 24.6°C (24.2- 25.1 °C}
- 9. Physical I Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyyille, CA).
Page 4 of98
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1. Results of a Pimephales pro melas Chronic/ 7 -day Toxicity Test.
(Genus species) (Type I Duration) Conducted February 10 - 17, 2009 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used- days) (%Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 98 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 98 Mean Dry Weight (mg) Test Solutions (replicate number) (% Effluent) 1 2 3 4 Mean Control 0.644 0.678 0.597 0.563 0.621 11.3% 0.630 0.628 0.616 0.683 0.639 22.6% 0.670 0.679 0.612 0.743 0.676 45.2% 0.614 0.619 0.693 0.525 0.613 72.6% 0.577 0.522 0.658 0.579 0.584 100.0% 0.605 0.742 0.593 0.534 0.619 Intake 0.622 0.612 0.599 0.553 0.597 ICzs Value: > 100% Calculated TU Estimates: < 1.0TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LCso: TUc = 100/ ICzs Page 5 of98
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphnia dubio Chronic/ 7-day Toxicity Test.
(Genus species) (Type I Duration) Conducted February 10- 16, 2009 using effluent from Outfall 101. Percent Surviving Test (time interval used- days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Reproduction (#young/female/6 days) Test Solutions Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 34 31 31 30 34 35 29 31 33 32 32.0 11.3% 33 33 35 31 35 34 30 32 31 . 32 32.6 22.6% 35 31 33 32 35 35 31 35 33 35 33.5 45.2% 35 35 33 32 36 35 35 33 35 33 34.2 72.6% 35 34 37 34 35 38 36 37 36 34 35.6 100.0% 37 34 39 38 36 39 36 35 39 36 36.9 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Pennit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LCso: TUc = 100/ ICzs Page 6 of98
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphnia dubia Chronic/7-day Toxicity Test.
(Genus species) (Type I Duration) Conducted February 10- 16, 2009 using water from Intake Percent Surviving Test (time interval used- days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/6 days) Test Solutions Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 32 31 31 33 33 31 33 32 31 30 31.7 Intake 34 37 35 33 37 40 35 34 37 35 35.7 IC2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LC5o: TUc = 100/ IC2s Page 7 of98
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type I Duration) Conducted February 10- 17,2009 using effluent from UV Treated Outfall101. Test Percent Surviving Solutions (time interval used- days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg) Test Solutions (replicate number) (% Effluent) 1 2 3 4 Mean Control 0.611 0.630 0.610 0.665 0.629 11.3% 0.642 0.720 0.729 0.747 0.710 22.6% 0.664 0.740 0.636 0.663 0.676 45.2% 0.716 0.622 0.516 0.577 0.608 72.6% 0.642 0.571 0.639 0.676 0.632 100.0% 0.626 0.654 0.647 0.688 0.654 Intake 0.567 0.601 0.586 0.717 0.618 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LCso: TUc = 100/ IC2s REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration Toxicant Results (IC2s) Pimephales promelas February 10-17, 2009 1130 7-days KCl 0.83 giL Ceriodaphnia dubia February 3-9, 2009 0750 6-days NaCI 1.05 g/L Page 8 of98
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimepholes promelos and Ceriodophnio dubio Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfull 101 performed February I 0-17, 2009. Test Sample ID Temperature ( 0 C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual ; Initial Final Initial Final initial Final (f.tmhos/cm) (mg/L CaCO~ (mg/L CaC0 3) Chlorine (mg/L) I 24.7 24.5 7.7 7.4 7.78 7.69 324 63 95 - Control 24.6 - 24.8 24.2 - 24.7 7.5 8.2 6.6 7.7 7.69 - 8.01 7.44 - 7.81 306 345 62 - 64 92 98
- 11.3%
24.7 24.5 7.9 7.4 7.85 7.66 310 - - -
,., 24.7 - 24.9 24.1 - 24.7 7.7 8.4 6.6 - 7.8 7.80 - 7.88 7.30 - 7.80 300 332 -
~ 22.6%
24.8 24.5 7.9 7.4 7.86 7.67 292 - - -
!; 24.7 - 24.9 24.3 - 24.7 7.7 - 8.4 6.2 - 7.8 7.81 - 7.89 7.38 - 7.79 282 300 - - -
'I:>..
45.2% 24.8 24.4 7.9 7.4 7.87 7.67 269 - - -
~ 24.7 - 24.9 24.2 - 24.5 7.7 - 8.5 6.3 7.7 7.81 - 7.90 7.38 - 7.81 262 - 275 - - - -
-I:
24.8 24.4 8.0 7.4 7.88 7.70 240 - - -
~ 72.6%
24.7 - 24.9 24.2 - 24.6 7.7 - 8.5 6.3 - 7.7 7.81 - 7.92 7.39 - 7.83 233 - 245 - - -
~ 100.0%
24.8 24.4 8.0 7.3 7.89 7.71 211 73 85 < 0.10 24.7 - 24.9 24.2 - 24.6 7.7 - 8.5 6.3 7.7 7.81 - 7.94 7.40 - 7.83 204 - 218 70 - 75 82 86 < 0.10 - <0.10 24.8 24.4 8.0 7.4 7.90 7.71 211 73 86 < 0.10 Intake 24.7 - 24.9 24.2 - 24.5 7.7 - 8.5 6.4 7.7 7.85 - 7.95 7.40 - 7.84 206 - 218 72 - 74 82 92 < 0.10 - <0.10 Control 24.7 24.8 7.7 7.8 7.79 7.81 321 63 95 - 24.5 - 24.8 24.6 - 25.0 7.5 8.2 7.5 8.2 7.69 - 8.01 7.75 - 7.89 306 325 62 - 64 92 98 11.3% 24.8 24.8 7.9 7.8 7.85 7.80 309 - - - 24.7 - 25.0 24.6 - 24.9 7.7 - 8.4 7.6 - 8.2 7.80 - 7.88 7.75 - 7.88 300 332 - - -
""' 22.6%
24.8 24.9 7.9 7.8 7.87 7.80 291 - - -
.§ 24.7 - 25.0 24.8 - 25.0 7.7 - 8.4 7.6 8.2 7.81 - 7.89 7.74 - 7.86 282 300 - -
-:! 24.8 24.8 8.0 7.8 7.88 7.82 268 - - -
- 45.2%
-I:
24.7 - 25.0 24.7 - 25.1 7.7 - 8.5 7.6 8.2 7.81 - 7.90 7.75 - 7.87 262 - 27 5 -
~ 24.8 24.8 8.1 7.7 7.89 7.82 239 - - -
*;::"' 72.6%
24.7 - 25.0 24.7 - 24.9 7.8 8.5 7.5 8.2 7.81 - 7.92 7.75 - 7.87 233 245 - - -
\,)
24.9 24.8 8.0 7.7 7.90 7.84 211 73 85 < 0.10 100.0% 24.7 - 25.1 24.6 - 24.9 7.7 - 8.5 7.5 - 8.0 7.81 - 7.94 7.76 - 7.89 204 - 218 70 75 82 86 < 0.10 - <0.10 24.7 24.8 8.1 7.7 7.91 7.85 210 73 86 < 0.10 Intake 24.6 - 24.9 24.6 - 25.0 7.7 - 8.5 7.6 - 8.1 7.85 - 7.95 7.76 - 7.90 206 217 72 74 82 92 < 0.10 - <0.10 Overall temperature ('C) Average Minimum Maximum Pimepholes promelos 24.6 24.1 24.9 Ceriodophnio dubio 24.8 24.5 25.1 Page 9 of98
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistty Mean Values aud Ranges for Pimephales promelas Tests, UV-treated Sequoyah Nuclear Plaut (SQN) Outfall 101 performed February 10-17, 2009. Test Sample lD Temperature ("C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Initial Final Initial Final Initial Final (1-1mhos/cm) (mg/L CaC0 3) (mg/L CaC0 3 ) 24.7 24.6 7.9 7.4 7.83 7.54 310 62 91 Control 24.6 - 24.8 24.5 - 24.6 7.6 - 8.5 6.7 - 7.8 7.74 - 7.91 7.06 - 7.73 303 - 318 61 - 63 90 - 94 11.3% 24.8 24.5 7.9 7.3 7.76 7.54 304 - - 24.7 - 24.9 24.3 - 24.6 7.6 - 8.5 6.6 - 7.6 7.15 - 7.91 7.04 - 7.76 296 - 313 - - - -
~"' 22.6%
24.8 24.4 7.9 7.3 7.76 7.55 293 - -
..c£::
~
24.7 - 25.0 24.8 24.2 - 24.7 24.4 7.6 - 7.9 8.5 6.5 - 7.4 7.8 7.15 - 7.91 7.04 - 7.75 7.77 7.57 287 - 303 271
~
~
~
45.2% 72.6% 24.7 - 25.0 24.8 24.2 - 24.7 24.4 7.7 - 8.5 8.0 6.5 - 7.3 7.8 7.15 - 7.91 703 - 7.74 7.77 7.58 263 - 279 239
.§ 24.7 - 25.0 24.2 - 24.7 7.7 - 8.5 6.4 - 7.7 7.15 - 7.92 7.02 - 7.78 234 - 245 - - - -
~ 85 24.9 24.4 8.0 74 7.78 7.59 211 71 100.0%
24.7 - 25.1 24.2 - 24.6 7.7 - 8.4 6.4 - 7.7 7.14 - 7.93 7.02 - 7.77 204 - 218 70 - 72 t\4 - 86 24.9 24.5 8.0 7.2 7.75 7.58 209 72 85 Intake 24.7 - 25.0 24.2 - 24.7 7.8 - 8.7__ 6.4 - 7.4 7.14 - 7.92 _6.99 - 7.83 197 - 216 69 - 74 84 - 86 Overall temperature ("C) Average Minimum Maximum Pimephales promelas 24.6 24.2 25.1 Page 10 of98
SUMMARY
I CONCLUSIONS Outfall101 samples collected February 8-13,2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were
> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 11 of 98
Appendix A ADDITIONALTOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None Page 12 of98
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B.
- 4. Dissolved oxygen was measured by SM 4500 0 G.
- 5. The pH was measured by SM 4500 H+ B.
- 6. Conductance was measured by SM 2510 B.
- 7. Alkalinity was measured by SM 2320 B.
- 8. Total hardness was measured by SM 2340 C.
- 9. Total residual chlorine was measured by ORION Electrode Method 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC 25 values in giL KCl or N aCl.
- 2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 13 of 98
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Standard Methods for the Examination of Water and Wastewater, 20th Edition, 1998.
\
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of98
Sequoyah Nuclear Plant Biomonitoring February 10- 17, 2009 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of98
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- February 13,2009 PCL-401 mg/L Copolymer 0.005 0.011 0.021 0.019 0.015 0.015 0.015 0.024 0.024 0.024 0.024 0.024 0.024 0.023 Page 16 of98
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- February 13, 2009 Date PCL-401 mg/L Copolymer 01/31/2000 0.009 02/01/2000 0.028 02/02/2000 0.009 02/03/2000 0.009 02/04/2000 0.009 02/05/2000 0.009 02/06/2000 0.009 0.019 0.019 0.018 0.019 0.019 0.019 0.019 12/11/2000 0.020 12/12/2000 0.020 12/13/2000 0.020 12/14/2000 0.020 12115/2000 0.020 12/16/2000 0.020 12117/2000 0.020 08/26/2001 0.021 08/27/2001 0.021 08/28/2001 0.021 08/29/2001 0.020 08/30/2001 0.021 08/31/2001 0.020 11/25/2001 11/26/2001 0.02 11/27/2001 0.019 11/28/2001 0.019 11/29/2001 0.02 11130/2001 0.02 12/09/2001 12/10/2001 12/11/2001 12/12/2001 0.02 12/13/2001 0.02 12/14/2001 0.02 Page 17 of98
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - February 13, 2009 Date PCL-401 mg/L Copolymer 01/02/2002 0.02 01/03/2002 0.014 01/04/2002 0.014 01/05/2002 01/06/2002 01107/2002 0.014 02/24/2002 02/25/2002 0.023 02/26/2002 0.023 02/27/2002 0.023 02/28/2002 0.008 03/01/2002 . 0.008 05/05/2002 ...* 05/06/2002 0.02 05/07/2002 . 0.02 05/08/2002 0.019 05/09/2002 0.02 05/10/2002 0.019 08/04/2002 08/05/2002 0.018 08/06/2002 0.018 08/07/2002 0.019 08/08/2002 0.019 08/09/2002 0.018 10/06/2002 10/07/2002 0.018 10/08/2002 0.018 10/09/2002 0.018 10/10/2002 0.018 10/11/2 002 F:***:*:***:*:*:*:*:***:********* 0.018 01112/2003 01/13/2003 0.019 01/14/2003 0.020 01/15/2003 0.020 01/16/2003 0.020 01/17/2003 0.009 04/06/2003 04/07/2003 0.021 04/08/2003 0.021 04/09/2003 0.021 04/10/2003 0.021 04/11/2003 0.022 Page 18 of98
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-February 13,2009 Date PCL-401 mg/L Copolymer 06/15/2003 06/16/2003 0.020 06/17/2003 .. 0.014 06/18/2003 0.014 06/19/2003 0.020 06/20/2003 0.020 08/03/2003 08/04/2003 0.020 08/05/2003 0.020 08/06/2003 : 0.020 08/07/2003 0.020 08/08/2003 0.020 10/05/2003 0.020 10/06/2003 0.020 10/07/2003 0.020 10/08/2003 0.020 10/09/2003 0.022 10/10/2003 0.024 02/01/2004 0.009 02/02/2004 0.009 02/03/2004 0.009 02/04/2004 0.009 02/05/2004 0.009 02/06/2004 0.009 05/04/2004 0.019 05/05/2004 0.014 05/06/2004 0.013 05/07/2004 . 0.020 05/08/2004 0.021 05/09/2004 0.020 07/04/2004 0.019 07/05/2004 0.020 07/06/2004 0.020 07/07/2004 0.019 07/08/2004 0.019 07/09/2004 0.020 Page 19 of 98 .
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12,1998 -February 13,2009 Date 11/07/2004 11108/2004 11109/2004 11110/2004 .. 11111/2004 11/12/2004 02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/11/2005 06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10/2005 07/17/2005 07/18/2005 07/19/2005 07/20/2005 07/2112005 07/22/2005 10/30/2005 10/31/2005 11/01/2005 11/02/2005 11/03/2005 11/04/2005 11/14/2005 *.. 11/15/2005 11/16/2005 11117/2005 11/18/2005 11/19/2005 Page 20 of98
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998- February 13,2009 Date Cuprostat-PF mg/L Azole 11/12/2006 11/13/2006 11114/2006 11/15/2006 11116/2006 11117/2006 11126/2006 11/27/2006 11128/2006 11/29/2006 11130/2006 12/01/2006 05/28/07 0.015 05/29/07 0.015 05/30/07 0.015 05/31/07 O.D15 06/01/07 0.015 06/02/07 0.015 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/13/08 04/14/08 04/15/08 04/16/08 04/17/08 04/18/08 10/26/08 10/27/08 10/28/08 10/29/08 0.030 10/30/08 0.030 10/31/08 0.030 Page 21 of98
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Conosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -February 13,2009 Date Cuprostat-PF H:F<~~~*nu mg/L Azole 02/08/09 02/09/09 02/10/09 02/11/09 02/12/09 02/13/09 Page 22 of98
Sequoyah Nuclear Plant Biomonitoring February 10- 17, 2009 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 23 of 98
'"C ~
1.10 tt>
~
s BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 ofl
~ Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery
/J Facility Sampled: Sequoyah NP 28801 Geneml ~o~:~ !J/Cf~¢1 Chevy Wtlhamsv . , . .- / .e~
Phone: 828-350-9364 Ben Mitchell : c~~ ~~~_/' NPDES Number: TN0026450
.........__~~~ -..,...,.-,..........-/':.::7 Collected By: Chevy Williams, Ben Mitchell Fax: 828-350-9368 Dissolved Metals Collecte . Samples remained on ice through out sampling and tmnsport to Jab.
Field Identification I Grab/Comp. vJ-~524~ iJit Collection Date/Time Container Rain Event? Sample Description Number& Volume ) (Mark as Appropriate) fYWJ Laboratory Use Collected IJ~".~r{:,,r*,.~l~*.~:;\f~::::;'tl:r .;'*'n);~-~.Cr:**;::. f'.-::~~1.~,s~i;;"~~~} ~*-5.i:.3ft*;S Date Time Yes If Yes, No Trace ETSLog Arrival By Time Appear-(EST) Inches Number Temp. ance I'< <./ )*"* ~f. :c. .jf.' oc) E\ SQN-101-TOX Comp 02/08/09 -02/09/09 0921- 2 (2.5gal) ~a*- X '\'C..
~ozD".o\ (\\ \ss'-\
Sl4i..ll.Ci 0821 ~:~ :: \SS"l 1-\<7-t> ,.1 I* SQN-INT-TOX Comp 02/08/09 -02/09/09 0940-0840 1 (2.5 gal) NA Sample Custody- Fill In From Top Down X Oill"'Z ~ .L1l-
~ <.v.ft ceLt
'2.A*c..
A D ,.jJ A
>> \Ss*-\
~,..A.
~
'** t..A-.90001. ......
Relinquished By (Signature): Date/Time Received By (Signature):
'-J" Ablll.u.JoUI(
Date/Time d"" ...J)_ ChevyWi~ 02/09/09 1f: 5~ ESl"' Sonic Delivery 02/09/09 lJ;fJ~ Jlo-~ E\ Sonic Delivelr ~ ~ ET:~;~ 02/09/09 02/09/09
,c;sL\ E\ ET<;. tSSY ~
~
Instructjons: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
~ ~
I1Q (t>
~ BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 1 °~------------------------------------~------------------------------------~~-------------------------------------'
Client: TV A Environmental Testing Solution, Inc. Delivered By (Circle One): l Project Name: Sequoyah NP Toxicity 3 51 Depot Street. FedEx UPS Bus Client Asheville, NC Other (specify): Sonic D~livery: . 1 P.O. Number: N/A / Facility Sampled: Sequoyah NP 28801 General ~o~en~~ in>-~ 1 / fl1 / ./'? Chevy Wdhams:~(""'""".1'-::f:- ti1~/~A,.t--~:,~/~~VY..___;(...,_i~;:z.----t-/:.___ NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell : ::-)",.../..;-'":~ ~ *.r-.1
- Collected By: Chevy Williams, Ben Mitchell Fax: 828-3 50-93 68 Dissolved Metals Collected. Samples remained on ice through out sampling and transport to lab.
Field Identification I Grab/Comp. Collection Dateffime Container Flow . 5.2. 4~ Sample Description Number & cfs Ram Event? ~.-ClU-~r#. La borat ory Use Volume (Mark as Appropriate) Collected
~~: }\fS*;)i;.: <: [J l;,) :;:+~?* -:,;/ Date Time :':Y~~:,~~;~:sl:~T>: ':*\[-,#"::'*: Yes If Yes, No Trace ETS Log Arrival By Time Appear-
~~2:t.~2,,,,,;, ,,, :_al_ .-. :.>*J.~. :. (EST) ;~:;;~~.f.::*::;:¥:.i;:~:.:;;,"*.s:. Inches Number ~~~* E\ ance SQN-101-TOX Comp 02/10/09-02/11109 0915- 2 (2.5gal) 2464- X 0815 2494 \*\~ 'Z ~11.1,."(; \ L~"> .J.
AVG= tf\'V~ ** (j\ \? -'f"' 2471 = \"Y\11~C:rt> SQN-INT-TOX Comp 02/10/09-02/11109 0936- I (2.5 gal) NA X \*'4
- S~ i,.
0836 <:9\~'J.-\ 3.\ (_ (} ,~ Sample Custody- Fill In From Top Down -Af. ~~ ~ - *-ll - _&. -t~~
'..,,, ... lid. '-""-~~
Relinquished By (Signature): Date/Time Received By (Signature): Dateflime ( fv-- ...... Chevy Williamy. j ~ 4 / ,f/ 02/11/09 . .* _ . Sonic Delivery 02/ll/09 c_/9//} t~Gtyz~( II .;<gc5r bIt ~ I;: l<t \ I
~ .IJ 1
Sonic Delivery L) ./ (? 02/11/09 ETS " I E'TS 02/11/09
~~ ~ ISS~ E"t"" {)wt/~ lSS3 ET
'- \J 7 Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive untiHbc:~llowing Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1oft Client: TV A Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Nwnber: N/A Asheville, NC Other (specify): So~~vet')'
/
/!
Facility Sampled: Sequoyah NP 28801 GeneralComm~ Chevy Williams{ ' * /' . NPDES Nwnber: TN0026450 Phone: 828-350-9364 Ben Mitchell :~-==:= =cz Collected By: Chevy Williams, Ben Mitchell Fax: 828-350-9368 Dissolved Metals Collected. Samples remained on ice through out sampling and transport to lab. Field Identification I 1 Grab/Comp. Sample Description Collection Date/Time Container Number& I Flow cfs Rain Event? p~ e,(1iL 'SZL{(p Laboratory Use Volume (Mark as Appropriate) Collected Date I Time (EST) Yes IfYes, Inches I No I Trace ETS Log Number I Arrival Temp. I I e:. I By Time T Appear-ance SQN-101-TOX I Comp o21121o9 -02113109 1 o936-os36 2 (2.5gal) I 2453- I 2493 AVG= I I X
~,!>b I. :J:.s*~ l,t\ I ~*
2470 *:hs~~.-,6-t;) SQN-INT-TOX I Comp 02/12109 -02113109 1 os42-o742 1 (2.5 gal) I NA I I I X
~o~\)1-'?:1~ I 0 .1*(.. I0 J' ,t;,';\ I -If.
Sample Custody- Fill In From Top Down ~u~Ooet~~~.
- A Relinquished By (Signature): Date/Time Received By (Signature):
so .. p~~~ Date/Time cJ
/I '!0 ESr ISonic N ~
fJ Delivery 02/13/09 YTJ12Jdvtll
--- lf2 -- /
02113/09 I I(' I~ E\
~
02/13/09 ETS 02/13/09 Sonic Delive;lg 1'S~\ ET 1 r~~~ l"S~\ E.\
~
Pagel of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: TVA County: Hamilton Facility: Seguoyab Nuclear Plant Treatment: Non-treated NPDES #: TN 0026450 Outfall: 101 Project #: __..S~-z.,_"!.Ll!Q...______ Dilution preparation information: Comments: Dilution prep (%) !1.3 22.6 45.2 72.6 100 Effluent volume (rnL) 282.5 565 1130 1815 2500 Diluent volume (rnL) 2217.5 1935 !370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test or~:anism information: Test information: Organism age: ,q . 11 l*hu Its o 1..{) Randomizing tem.Jl.late: 'I eu.c IQ Date and times organisms 01.-o~-o~ \\coO Incubator number: were born between: 3C Organism source: A--ro"i-. & 1'\\\.~ {J,P Ol*tl\*cf\ Artemia lot number: ~lro'i~A) Transfer bowl information: pH= Temperature= *c Total drying time: 'L"l* \\c.uit~ 1.* (,1. 1."l*~ Date I Time in: o 1* 11*o'\ 1\\D Average transfer volume: Date I Time out:tn.*\!*ct\
- o. ~~~_Q Oven temperature:
\\ \D
'cC"t Daily feeding and renewal information:
Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time batch used used time 0 2 3 4 I 5 I I* 6 I I 7
~ ~age 27 of98 ...
Page 2 of 6 10 Species: Pimephales promelas Date: -~o1....~o.-___:.~_!.*-=O~~_!___ _,;.._ Client: TVA I Sequoyah Nuclear Plant* Non-treated Survival and Growth Data Day CONTROL 11.3% 22.6% A B c D E F G H I J K L 0
/t) /D 10 I{) ID to ID 10 ID ID 10 10 I
/0 tb 10 IC IO 10 10 ID tC 10 10 10 2
/D 10 IO l() 10 10 10 lD ID I(J LD ID 3
10 ID /D /0 I() It) I() I() I() 10 /Q 10 4 10 10 10 10 IQ IO 10 10 10 10 tO 10 5
/0 10 10 10 ID 10 IO /0 (0 lO IO 10 6
/D /0 /0 /0 10 /0 /0 to /Q 10 /() 10 7
/0 IO lf:::J 1\J 10 10 fC to /\) fD 10 l't; A= Pan weight (mg)
Tray color code:: ~{.\\cu..) Analyst: U1(b 1tf :~1. 1"!.1.8 13.1.{1 lt.{.CS t3.1..D lt3.'11 1"\.11 15.'10 1~.51 t"{.to l~.lf~ 1~.15 Date: Cl* \1'\:-CI"' B =Pan + Larvae weight (mg) Analyst: f"Lr IJZ>*1-b l\.M 1~.~s 11.b8 11.50 IJ.b. i Jb.33 :u.:n I;J.\, ?-1 ,;}.!). q~ ;)b, ~I J.\.}8 Date: ol. *I~ C = Larvae weight (mg)
*o"
=A-B l,.t.t"' 1,.18 S.'\, s.~.t~ (,.~ lA* '22 (o.ll.. c..&~ '-*1b 1..""
..... n. vt~
Weight per initial number
...,,., ~
ft.,., ,..,o ,'V ~~ o*""'
...,:t- ~ ::!'
oflarvae (mg)
~
= C I Initial number of larvae
()' VI (f o' 0
\0 o' o*
v
"'" ..sl C)
'I) o* o'
\0 \t
()'
~ f\
()' Average Percent weight per reduction initial from control o.!Q~'\ - 3.() 7. o.~1t.. - g.~1. number of (%) 0 *"' 'Z.\ larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: .J\__ Comments: ge 28 of98
~: Page 3 of6 ~ (Ill ~
- )e
- _n*-h*n-m"-'*'-Ten-Jng-Sal-"'lon-,,-,n<.
Species: Pimephales promelas Date: <fk IO-o\ Client: TVA I Sequoyah Nuclear Plant- Non-treated Survival and Growth Data Day 45.2% 72.6% 100% M N 0 p Q R s T u v w X 0
/{) /() ID /{J /Q 10 /Q /D fO 10 'D 10 1
/D /{) 10 /0 10 10 10 tO 10 10 1/J 10 2
rD 10 JD It> 10 tO 10 tO lt> lO I () ID 3
/0 10 /D JQ /Q JO lD ID 10 /0 10 10 4
J() IO fb IO tO I() fD tO ID 10 /() 10 5 10 LO 10 ID 10 10 10 10 IO IO IO 10 6
/0 /() /0 10 ID 10 /Q /D /D /D /0 10 7
IO 10 10 4'c( /0 /Q*~ 10 10 IO /o ID tQ lSI'\ A- Pan weight (mg) Tray color cod~How Analyst: 15.S9 I"{.S1 *o.'U. 1\.f.lB 1'{.35 1'1.9.1 1~.":11 13.S(p 13 ~'I 13.SS 13.83 ll.f.31o Date: (\~-lf\-rA B- Pan +Larvae weight (mg)
,..,..Lr l,\. +1 ~.,., 'Jb.SS d.D.l>J ~. IJ. l.D*I3 ).D. I) ,q,f>) dJ>.Oij )o.il+ Itt. tb l~*+o Analyst:
Date: DJ..* 13
- 0~
C - Larvae weight (mg)
=A-B
~ -s ,,'\ S.'\~ S.!.'-t
"'*' "' c.*'" '-.I\?, ;.tS s.l't ~..~i lcJ.OS ,.'-\1.
6,,, Weight per initial number ;,._'V of larvae (mg) ~ 0..~ c;~ "'"" o*... c,"' ~ bot, c..,.,,., ~~ t; 4
= C I Initial number of larvae o*
\JI
()'
~*"' o*""'" ()' ~:~* o*
\1 C>. ()"""' ()' o*
Average Percent weight per reduction initial from control o.~o\3, \.7.1. o.-s&'-1 S.'\7. o.\.,1'\ o.o7. number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. . Calculations and data reviewed: j,__ Comments:
*** Page 4 of6 tl
'iii
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~E-,.,-,.,-m,-,.. .... -,..-.,..-.,.-,ln<. Species: Pimephales promelas Date: -~O~'l~*~IOr-.*(\~".L---_.. Client: TVA I Sequoyah Nuclear Plant- Non-treated Survival and Growth Data Day 100% Intake y z AA BB 0 ID I() ID IO 1 10 10 10 10 2
/D 10 10 LO 3
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to IC 10 '1'.{ A= P11n weight (mF.Jc~",'-;r Tray color code:: ':f-QES N.OB 13.'\0 ILlY 1~.3q Analyst: ~ Date: 07.-\ Q-()CI B =Pan+ Larvae weight (mg) lo.30 ;}.b. OJ. 1~*~3 Jtt,ql Analyst: (v\Lt" Date: o~-1'8 -oq C =Larvae weight (mg)
=A-B e,.."t't "'. \z. s.'i~ s.s~
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= C I Initial number of larvae Average Percent
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()' ()' o* weight per reduction initial from control 0 .sq1 3 .'\i. number of (%) larvae (mg) Comment codes: c =clear, d = dead, fg = fungus, k = killed, m =missing, sk = sick, sm =unusually small, lg = unusually large, d&r =decanted and returned, w = wounded. Calculations and data reviewed: .dJ._ Comments:
'"C ~ ~ TVA I Sequoyah Nuclear Plant, Outfall lOt -Non-treated ~ February 10-17,2009 (.;.) Q \C QC) Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0
~
Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
~ Environmental Testing Solutions, Inc Project *umber* 5246
---*-***---.. r \ ~
Not for Compliance Assessment, Internal Laboratory QC ~ Coaceatralioa (%) Replicate lailialaamber of Fiaal **mber of larvae A*Pu,..;pt (me) B*Paa-tLanK welpt(mg) Larvu wac** (me)
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Mae sarvival ("'o) Mcaal\'Cight/ lailial .. mber or Coel cicot of varialioa 1M- ...ap. PtrU:D1 rtdudiotl fr0111 cntruJ("l'*) la,....(mg) pviAiliU,...*t>oorofla,...u) l%1 A 10 10 14.32 20.76 6.44 0.644 0.644 B 10 10 14.28 21.06 6.78 0.678 0.621 8.2 0.678 Control 100.0 0.621 8.2 Not applicable c 10 10 13.41 19.38 5.91 0.597 0.597 D 10 10 14.05 19.68 5.63 0.563 0.563 E 10 lO 13.20 19.50 6.30 0.630 0.630 F 10 JO 13.91 20.19 6.28 0 628 0.628 11.3~. 0.639 4.7 100.0 0.639 4.7 .J.O G 10 10 14.17 20.33 6.16 0.616 0.616 H 10 10 15.90 22.73 6.83 0.683 0.683 I 10 10 14.51 21.21 6.70 0.670 0.670 J 10 10 14.20 20.99 6.79 0.679 0.679 22.6% 0.676 7.9 100.0 0.676 7.9 -8.9 K 10 10 14.49 20.61 6.12 0.612 0612 L 10 10 13.75 2l.l8 7.43 0.743 0.743 M 10 10 15.59 21.73 6.14 0.614 0.614 N 10 10 14.57 20.76 6.19 0.619 0.619 45,2o/a 0.627 7.4 97.5 0.613 11.2 1.2 0 10 10 13.92 20.85 6.93 0.693 0.693 p 10 9 14.78 20.03 5.25 0.583 0.525 (J 10 10 14.35 20.12 5.77 0.577 0.577 R 10 10 14.91 20.13 5.22 0.522 0 522 72.6~. 0.584 9.6 100.0 0.584 9.6 5.9 s 10 10 13.57 20.15 6.58 0.6.58 0.658 T 10 10 13.86 19.65 5.79 0.579 0.519 u 10 10 13.99 20.04 6.05 0.605 0.605 100°/e v 10 10 13.55 20.97 7.42 0.742 0.6!9 14.2 0.742 100.0 0.619 14.2 0.3 w 10 10 13.83 19.76 5.93 0.593 0.593 X 10 10 14.36 19.70 5.34 0.534 0.534 y 10 10 14.08 20.30 6.22 0.622 0.622 J oocv. lotake z 10 10 13.90 20.02 6.12 0.612 0612 1.6 0.612 97.5 0.597 5.1 3.9 AA 10 10 12.74 18.73 5.99 0.599 0.599 BB 10 9 14.39 19.92 5.53 0.614 0.553 OotraliJOl: MSD~ Minimum Significant Difference Dunnett's MSD -valae: 0.1030 PMSD* Percent Minimum Significant Difference PMSD: 16.6 PMSD is a measure of test precision. The PMSD is the m1mmum percent difference between the control and treatment that can be declared statistically significant m a whole effluent toXICity test
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Dunnett's MSD value: 0.0575 Lower PMSD bound detennined by USEPA (lOth percentile) ~ 12%. PMSD: 9.3 UJlper PMSD bound detennined by USEPA (90th percentile)= 30%. Lower and upper PMSD bounds were detennined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET lnterlabo~~ory VariabilitY,Study (USEP A, 200 Ia; USEP A, 200 I b) USEPA. 2001 a, 200Jb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
""C
.*.ETS
~ ~ ~ TVA I Sequoyah Nuclear Plant, OutfalllOl -Non-treated ~ N February 10-17, 2009 Q """l \0 QCI
- Statistical Analyses 4,. " *.**""-. #, ---
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Larval Fish Growth and Survival Test-7 Day Growth Start Date: 2/10/2009 Test ID: PpFRCR Sample ill: TVA I Sequoyah Nuclear Plant, Outfall 101 End Date: 2/17/2009 LabiD: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% 1 2 3 4 D-Control 0.6440 0.6780 0.5970 0.5630 11.3 0.6300 0.6280 0.6160 0.6830 22.6 0.6700 0.6790 0.6120 0.7430 45.2 0.6140 0.6190 0.6930 0.5250 72.6 0.5770 0.5220 0.6580 0.5790 100 0.6050 0.7420 0.5930 0.5340 Transform: Untransforlned !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6205 1.0000 0.6205 0.5630 0.6780 8.174 4 0.6453 1.0000 11.3 0.6393 1.0302 0.6393 0.6160 0.6830 4.664 4 -0.439 ' 2.410 0.1030 0.6453 1.0000 22.6 0.6760 1.0894 0.6760 0.6120 0.7430 7.934 4 -1.298 2.410 0.1030 0.6453 1.0000 45.2 0.6128 0.9875 0.6128 0.5250 0.6930 11.220 4 0.181 2.410 0.1030 0.6128 0.9496 72.6 0.5840 0.9412 0.5840 0.5220 0.6580 9.582 4 0.854 2.410 0.1030 0.6013 0.9318 100 0.6185 0.9968 0.6185 0.5340 0.7420 14.226 4 0.047 2.410 0.1030 0.6013 0.9318 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01) 0.95722824 0.884 0.42652272 -0.0353657 Bartlett's Test indicates equal variances (p = 0.69) 3.06386232 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV 1U MSDu MSDp MSB MSE F-Prob df Dunnett'sTest 100 >100 I 0.10304414 0.1660663 0.00375797 0.00365631 0.43092009 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 95%CL(Exp) Skew IC05 45.035 ICIO >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqnl01_02-I0-09data
'"C ~ ~ (1) TVA I Sequoyah Nuclear Plant, Intake- Non-treated YJ February 10-17, 2009 iETS YJ Q \C QC) Statistical Analyses c) Environmental Testing Solutions. Inc. Larval Fish Growth and Survival Test-7 Da;t Growth Start Date: 2/10/2009 TestiD: PpFRCR SampleiD: TVA I Sequoyah Nuclear Plant, Intake End Date: 211712009 LabiD: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR.-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% 1 2 3 4 D-Control 0.6440 0.6780 0.5970 0.5630 100 0.6220 0.6120 0.5990 0.5530 Transform: Untransformed 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6205 1.0000 0.6205 0.5630 0.6780 8.174 4 0.6205 1.0000 100 0.5965 0.9613 0.5965 0.5530 0.6220 5.112 4 0.811 1.943 0.0575 0.5965 0.9613 Auxili~ Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95853066 0.749 -0.2030801 -0.8613815 F-Test indicates egual variances U! = 0.43l 2.76694155 47.4672279 Hypothesis Test ( 1-tail, 0.05) MSDu MSDE MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.05749647 0.09266151 0.001152 0.001751 0.44827035 1, 6 Treatments vs D-Control Linear Interpolation (200 Resamp1es) Point % SD 95%CL(Exp) Skew IC05 >iOo IC10 >100 ICI5 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqnl01_02-10-09data
""= ~ TVA I Sequoyah Nuclear Plant, OutfalllOl -Non-treated
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*-E Pimephales promelas Chronic Whole Effiuent Toxicity Test
- ~* EPA-821-R-02-013, Method 1000.0
< **',, -~ Daily Chemical Analyses
~ Environmental Testing Solutions, Inc. 5246 Concentration Control 11.3% 22.6% 45.2% 72.6% 100% 100% Intake
Page 5 of6 _) Envll'l)l'lmentiiTe-1tlng Solutions, Inc:. Species: Pimephales promelas Date: C1*10 *o'\ Client: TVA I Sequ()yah Nuclear Plant- Non-treated 11.3% 22.6% 45.2% I 72.6% I 100% I I If I 100% Intake II I lage 35 of98
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Species: Pimephales promelas Date: 01*lD-O'\ Client: TVA I Sequoyah Nuclear Plant- Non-treated Concen- Parameter tration 11.3% ~ 22.6% ~
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... Page 1 of7 11
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Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA County: Hamilton Facility: Sequoyah Nuclear Plant Treatment: Non-treated NPDES #: TN0026450 Outfall: 101 Project#: _....:5=2~~-=""'------- Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effluent volwne (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0 Total volume (mi..) 2500 2500 2500 2500 2500 Test orl(anism source information: Test information: Organism age: < 24-hours old Randomizing template color: OltP.~E. Date and rimes organisms were born 0'1* 0'\*t>'\ 10$0 'TI.) l't!J$ Incubator number and shelf between: location: 'Z.B\ Culture board: nt*CJ;..O'\ A Replicate number: I I 2 I 3 I 4 I 5 I 6 I 1 I 8 I 9 I 10 YWTbatch: 0\ *O~-cf\ Culture board cup number: ' I "'1..111..1 '"!:a I-' 11\oll~ I1'!JI'l.S l't-1 Transfer bowl information: pH= '1.1.:.1 SU Temperature= "t"\ ,, oc Selenastrum batch: ut~-o1l Daily renewal information: Day Date Test initiation, Control water Sample numbers Analyst renewal, or batch used used termination time 0 2 3 4 5 6 7 Control information: 1 2 Acceptance criteria Summary of test endpoints:
%of Male Adults: ~-2{)% ** 7-day LCSO )tM7.
% Adults having 3ra Broods:
% Mortality: , ***
Mean Offspring!FemaJe~ *.* :*
%CV: (...17. *~.* 3.37.
tage 37 of98
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*** Page 2 of7
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I' Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant- Non-treated Date: 0 Z*IO- o'\ I CONTROL-1 Day 1 2 3 Survival and Reproduction Data 4 Replicate number 5 6 7 8 9 10 o* I 1 2 Young produced Adult mortality Young produced 0
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~ ~ ~ *.~.
I % Mortality: Mean Offspring/Female: I 0~. I ~'2...o CONC: 11.3% Survival and Reproduction Data I Day 1 Young produced 1 a D 2 3 a 0 4 Replicate number 0 5 0 6 0 7 0 8 0 9 0 10 Adult mortality
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L 0 L 0 L 0 L L 0 L.. 0 L 0 L 0 0 L 0 3 Young produced D 0 0 a 0 0 I D C) Cl (') Adult mortality L l L... L L L L L \..._ '-- 4 Young produced \,Q '-\ 5 ~ ~ .'--\ '-\ '-\ 4 s Adult mortality L L- L- L. \,__ \._. \._ L- L '- I 5 Young produced \1. \1. *3 1\ 14
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<It Page3of7 110
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Species: Ceriodaphnia duhia Client: Seguoyah Nuclear Plant- Non-treated Date: __:cn::..;:__*.:..;f()~*-=o_,'\_ _ _ __ CONC: 22.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced Adult mortality (') 0 a n (') {") a 0 0 a L..
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2 Young produced 0 0 0 D C\ D 6 0 C'\ 0 Adult mortality L. \._ L L L L L \..._ l_ L 3 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L '- L L '\.._. \.._ L '- '- 4 Young produced I-{ ~ '-\ 'S 'S "5 "4 \.a ~ s Adult mortality L L L. L 5 Young produced \~
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7 Young produced .u Total young produced as .3\ 3.?,) 32. 35 3S .3\ ~5 ~~ 3S Final Adult Mortality \ \ L L L L.. '-- '- L l Note: Adult mortality (L =live, D =dead) Concentration:
% Mortality: 07.
Mean Offspring/Female: a-o.s
% Reduction from Control: *"\.1?.
CONC: 45.2% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced () 0 0 0 0 0 0 0 0 0 Adult mortality L L- \._ L L L L \..._ L L 2 Young produced 0 0 0 0 0 0 6 0 0 0 Adult mortality L L L L L L L L L '- 3 Young produced 0 0 D 0 D 0 a 0 () (\_ Adult mortality L L- L. \..._ L L L L L \.... 4 Young produced ..., "5 "-\ l.\ \p "-\ '.t. ~ s s Adult mortality L L L L \,_ L._ \,_
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~~ ~s 33 .32. ol.. ~s .35 ~3 35 ~
Final Adult Mortality L '- \. L L L \...... \,_ Note: Adult mortality. (L = live, D = dead) Concentration:
% Mortality: "
Q1. Mean Offspring/Female: .J'!._2
%Reduction from Control: ~e:..'l7.
Page 4 of7 Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant- Non-treated Date: OZ*fO*o'\ CONC: 72.6% Survival and Reproduction Data
*. Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced a D 0 0 0 0 L
D L 0 0 c Adult mortality L L L L L '--- L \..._ 2 Young produced 0 _0 D 0 0 0 (') () 0 0 Adult mortality \.... \._ L \.._. L.. L L.. L L. L 3 Young produced n 0 0 0 {) 0 0 0 0 0 4 Adult mortality Young produced L
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% Mortality: ()1.
Mean Offspring/Female: 3-5. to
%Reduction from Control: ~\\.~'1.
CONC* 100% Survival and Reproduction Data Replicate number IJ Day 1 Young produced 1 () 0 2 0 3 0 4 0 5 0 6 0 7 0 8 0 9 0 10 Adult mortality L- L L L L L L L L \_ ll 2 Young produced Adult mortality ()
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Note: Adult mortality (L =live, D =dead) Concentration:
% Mortality:** 07.
Mean Offspring/Female: .31.. 't
%Reduction from Control: -*~-37.
. age 40 of98 I
- Page 5 of7
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*f Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant- Non-treated Date: _ _;0'2~*_.:..;1o,._*_,.o,_~.___ __
CONTROL-2 Survival and Reproduction Data Replicate number Day 1 2 3 '4 5 6 7 8 9 10 1 Young produced n 0 0 D D
.__ () 0 0 0 0 Adult mortality L L L \...._ '-- '-- L_ L L 2 Young produced 0 0 0 0 a a a_ 0 a a Adult mortality L \....... L \.._ L L L. L L L 3 Young produced *a 0 D 0 0 a 0 0 a 0_
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I J Concentration:
% Mortality: I 1'11.
Mean Offspring/Female: I 31*1 coNe: 100% Intake Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced [') 0 a_ 0 a_ 0 0 a 0 a Adult mortality j._.. '-- L L L- L L L. L L I! 2 Young produced Adult mortality LJ
\.......
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_l '- \._ \._ \_
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u %Mortality: Mean Offspring/Female: 07.
.3$.1
%Reduction from Control:
- 1'2. .&.1..
~~Page 41 of 98
"'C
.ETS
~ ~ TVA I Sequoyah Nuclear Plant, OutfalllOl- Non-treated ~ ~ February 10-16,2009 N c ...... Verification of Ceriodaphnia Reproduction Totals
@ ;',ffi'fifi;.l!: '****." *'*!.:::' ;.-.
~ 00 ._) Envlronmenlal TesUng Solutions, Inc. Control-1 72.6% 11.3% 100% 22.6% Control-2 45.2% 100% Intake
~ ~
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TVA I Sequoyah Nuclear Plant, OutfalllOl -Non-treated w February 10-16,2009 0
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~ Ceriodaphnia dubio Chronic Whole Effluent Toxicity Test
** EPA-821-R-02-013, Method 1002.0
~
Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
~ Environmental Testl,ng SOflj~lons, Inc.
Project number: 5246 a'~*w;;*n~u UJ* r\1. II
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Concentration Replicate number Survival Average reproduction Co'tl'ffcient of Percent reduction from (%) (offspring/female) variation (Vo) pooled controls (%) (%) 3 4 5 6 7 8 9 10 I 2 Control-1 34 31 31 30 34 35 29 31 33 32 100 32.0 6.1 Not applicable 11.3% 33 33 35 31 35 34 30 32 31 32 100 32.6 5.3 -1.9 22.6% 35 31 33 32 35 35 31 35 33 35 100 33.5* 5.1 4.7 45.2% 35 35 33 32 36 35 35 33 35 33 100 34.2 3.8 -6.9 72.6% 35 34 37 34 35 38 36 37 36 34 100 35.6 4.0 -11.3 100% 37 34 39 38 36 39 36 35 39 36 100 36.9 4.9 -15.3 Control-2 32 31 31 33 33 31 33 32 31 30 100 31.7 3.3 Not applicable 100% Intake 34 37 35 33 37 40 35 34 37 35 100 35.7 5.8 -12.6 Outfall101: MSD= Minimum Significant Difference Dunnett's MSD value: 1.703 PMSD= Percent Minimum Significant Difference PMSD: 5.3 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
~
Duonett's MSD value: 1.269 LowerPMSD boWJd determined by USEPA (lOth percentile) = 13%. PMSD: 4.0 Upper PMSD bound determined by USEPA (90th percentile) = 4 7%. Lower and upper PMSD boWJds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 200lb). USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EP A-821-B-01-004 and EPA-821-B-0 1-005. US Environmental Protection Agency, Cincinnati, OH.
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Ceriod~lmia Survival and R!:£roduction Test-Reproduction Start Date: Vl0/2009 TestiD: CdFRCR SampleiD: TV A I Sequoyah Nuclear Plant, OUtfall 101
- End Date: 211712Q09 Lab ID: ETS*Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaplmia dubia Comments:
Cone-% 1 2 3 4 5 6 7 8 9 10 D-Control 34.000 31.000 31.000 30.000 34.000 35.000 29.000 31.000 33.000 32.000 tl.3 33.000 33.000 35.000 31.000 35.000 34.000 30.000 32.000 31.000 32.000 22.6 35.000 31.000 33.000 32.000 35.000 35.000 31.000 35.000 33.000 35.000 45.2 35.000 35.000 33.000 32.000 36.000 35.000 35.000 33.000 35.000 33.000 72.6 35.000 34.000 37.000 34.000 35.000 38.000 36.000 37.000 36.000 34.000 100 37.000 34.000 39.000 38.000 36.000 39.000 36.000 35.000 39.000 36.000 Transform: Untransfonned !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D*Control 32.000 1.0000 32.000 29.000 35.000 6.074 10 34.133 1.0000 11.3 32.600 - 1.0188 32.600 30.000 35.000 5.254 10 -0.806 2.287 1.703 34.133 1.0000 22.6 33.500 1.0469 33.500 31.000 35.000 5.122 10 -2.014 2.287 1.703 34.133 1.0000 45.2 34.200 1.0688 34.200 32.000 36.000 3.850 10 -2.954 2.287 1.703 34.133 1.0000 72.6 35.600 1.1125 35.600 34.000 38.000 4.016 10 -4.833 2.287 1.703 34.133 1.0000 100 36.900 1.1531 36.900 34.000 39.000 4.856 10 -6.578 2.287 1.703 34.133 1.0000 Auxili!!;!! Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > O.Q1) 0.864555061 1.035 -0.055127545 -1.11981957 Bartlett's Test indicates !:}Ual variances (I!= 0.88! 1.7678653 15.08627224 Hypothesis Test (1-tail, 0.05! NOEC LOEC ChV TU MSDu MSD2 MSB MSE F-Prob df Dunnett's Test 100 >100 1 1.703244044 0.053226376 34.22666667 2.774074074 5.4E-08 5,54 Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 95%CL Skew IC05 >100 IC10 >100
!CIS >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqnl01_02-10-09data
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0 Environmental Tasting Solutions, Inc. Ceriod!!£1mia Survival and R~roduction Test-R~roduction Start Date: 2/10/2009 Test ID: CdFRCR SampleiD: TVA I Sequoyah Nuclear Plant, Intake End Date: 2/17/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaplmia dubia Comments: Cone-% I 2 3 4 5 6 7 8 9 10 D-Control 32.000 31.000 31.000 33.000 33.000 31.000 33.000 32.000 31.000 30.000 100 34.000 37.000 35.000 33.000 37.000 40.000 35.000 34.000 37.000 35.000 Transform: Untransfonned !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 31.700 1.0000 31.700 30.000 33.000 3.342 10 33.700 1.0000 100 35.700 1.1262 35.700 33.000 40.000 5.763 10 -5.466 1.734 1.269 33.700 1.0000
- Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.901544869 0.868 0.809339798 1.387605747 F-Test indicates ~al variances (p =: 0.06) 3.772277117 6.541089535 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.26901657 0.040032068 80 2.677777778 3.4£-05 I, 18 Treatrrtents vs D-Control Linear Interpolation (200 Resamples)
Point % SD 95%CL Skew IC05 >100 IClO >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 ICSO >100 sqn101_02-10-09data
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Project number: 5246
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Control 11.3% 22.6% 45.2% 72.6% 100%
Page 6 of7 Species: Ceriodaphnta duhia Client: Seguoyah Nuclea.r Plant- Non-treated 1-
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11.3% 22.6% I 45.2% 72.6%
'100% .
100% Intake age 47 of98
Page 7 of7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant- Non-treated Date: Ci.-!o-d1 Concen-tration 11.3% 22.6% 45.2% 72.6% I I
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Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: TVA County: Hamilton Facility: Seguoyah Nuclear Plant Treatment: UV-treated NPDES #:. TN0026450 Outfall: 101 Project#: -*-=S::..:2i~4~.l,~** --~- Dilution preparation information: Comments: Dilution prep (%) l!J 22.6 45.2 72.6 100 Each concentration was treated Effluent volume (mL) 282.5 565 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2217.5 1935 1370 685 0 to remove_Qathf!genic Total volume (mL) 2500 2500 2500 2500 2500 interferences. Test orflanism information: Test information: Organism age: LC\ H.o\Jitc; oLD Randomizin_g_ tero_ll_late: &t..\)e. Date and times organisms Incubator number: were born between: O'l*0'\*0~ IbOO 3& Organism source: A'it:))(. &A\<.\\ (J p cn.-<f\-0'\ Artemia lot number: f6Jooc.fw Transfer bowl infonnation: pH= Temperature= oc Total drying time: .,... 1\()\ltC> l.(o 1.. 1."t*3 Date I Time in: ()t,. n*cf\ 1\\t) Average transfer volume: Date I Time out: Cl.*\A*Q'\ t\\0 c.\~&4 ...-Q. Oven temperature: \oc't. Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time batch used used time 0 2 3 4 5 6 7 49 of 98
Page2of6 Species: Pimephales promelas Date:
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Client: TVA /Sequoyah Nuclear Plant- UV-treated Survival and Growth Data Day CUNfROL 11.3% 22.6% A B c D E F G H I J K L 0 I'D IO 10 ID /Q /Q IO /Q to /Q ID IQ 1
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It> ID IO 10 10 ID 10 10 10 10 /0 I'D 3 lt:J IO 10 to /0 IO 10 IO 10 10 /6 tO 4 ID 10 /Q IO /Q 10 /Q 10 10 IO 10 10 5 IC tD 10 /0 ID ID /Q /Q ID /0 10 tO 6
/Q /() /0 /0 /Q /Q /Q /Q /(j IQ IO 10 7
10 10 10 /Q IO 10 10 10 ID 10 /0 10 A = Pan weight (mg) Tray color code:: OVo..N5-e Analyst: LA-e l"f.l(c II'{.~~ t3 .sa "\.\\~ It.! .II ll.~Dq lt{.\{3 13.eq IS.lcJ l4.3'-t 14.01 l5. 1.2. _!)_ate~ 0'2.-10-Dq B =Pan + Larvae weight (mg)
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Comment codes: c = clear, d dead, fg = fungus, k ==killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r == decanted and returned, w = wounded. Calculations and data revi~wed: L Comments: . * .. SO of98
Page 3 of6
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Species: Pimephales prome/as Date: cn.*to-o'\ Client: TVA I Sequoyah Nuclear Plant- UV-treated Survival and Growth Data Day 45.2% 72.6% 100% M N 0 p 0 R s T u v w X 0 -
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*Analyst: LM \~.lol lS.IQ; IS.'~l II{.I{S 13.l£l8 13.01 11-t.'B l\{.11 l'Llo~ t3.~S i .~u l'-\-'l.lq Date: DZ-IC\-f\C\
B = Pan + Larvae weight (mg) Analyst: ~L-r" ";).l*iJ ~.0} d0-Y8 )b. ;).d. ;)b. to ]8*1-l ;J.I.3J :1.~'11 ;;lb. ~0 J,D.4clf d-().18 ;;l.l-1'1 Date: o?.* 1:g-o~ C- Larvae weight (mg)
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JD 10 10 10 7 '~ l$f"t 10 ID 'D ID A= Pan weight (m~~k,; t Tray color code:: ""'""l(, . r Analyst: L&S \~.lot l'Z..c:q 13.~1 1~.'{3 Date: Ot-ln-nct B =Pan + Larvae weight (mg) Analyst: ~ c}c.~ IS',q, J1-53 ;)b.bo Date: ol--1'& ~ o~ C = Larvae weight (mg)
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!10 TVA /Sequoyah Nuclear Plant, OutfalllOl- UV-treated (t> Ul February 10-17,2009 w 0
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n~uweuuy: A _II_ 5246 Co.ea&ntio* (0/*) JlepiiW. Iaitialoumbuof lanK ,..,. Fiaala*llber rl A=Puwdpt (mg) B "" Po + Larvae wdg~t(mg) Larvae waa*t
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Not for Compliance Assessment. Internal Laboratory QC Wdght/SIIn'ivilog ou-..orbrvae(mg) Mcuweig.tf Surviviae ***ber brvoe(mg) of Codlicieac ofvariatioa (Wr*ll...-tpertar¥1ri*J
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"{,J A 10 10 14.76 20.87 6.11 0.611 0.611 B 10 10 14.34 20.64 6.30 0.630 0.630 Control 0.629 4.1 100.0 0.629 4.1 Not applicable c 10 10 13.58 19.68 6.10 0.610 0.610 D 10 10 14.14 20.79 6.65 0.665 0.665 E 10 10 14.11 20.53 6.42 0.642 0.642 F 10 10 11.69 18.89 7.20 0.720 0720 11.3% 0.710 6.5 100.0 0.710 6.5 -12.8 G 10 10 14.43 21.72 7.29 0.729 0.729 H 10 10 13.89 21.36 7.47 0.747 0.747 I lO tO 15.61 22.25 6.64 0.664 0.664 J 10 10 14.34 21.74 7.40 0.740 0.740 11.6% 0.676 6.6 100.0 0.676 6.6 -7.4 K 10 10 1407 20.43 6.36 0.636 0.636 L 10 10 13.22 19.85 6.63 0.663 0.663 M 10 10 14.67 21.83 7.16 0.716 0.716 N 10 10 15.85 22.07 6.22 0.622 0.622 45.2% 0.608 13 9 100.0 0.608 13.9 3.4 0 10 10 15.32 20.48 5.16 0.516 0.516 p 10 10 14.45 20.22 5.77 0.577 0.577 Q 10 10 13.68 20.10 6.42 0.642 0.642 R 10 10 13.01 18.72 5.71 0.571 0.571 72.6o/. 0.632 7.0 100.0 0.632 7.0 -0.5 s 10 10 14.93 21.32 6.39 0.639 0.639 T 10 10 14.71 21.47 6.76 0.676 0.676 u 10 10 14.64 20.90 6.26 0.626 0.626 100%
v 10 10 13.95 20.49 6.54 0.654 0.654 3.9 0.654 100.0 0.654 3.9 -3.9 w 10 10 14.41 20.88 6.47 0.647 0.647 X 10 10 14.26 2l.l4 6.88 0.688 0.688 y LO 10 14.62 20.29 5.67 0.567 0.567 100% latake z 10 10 12.97 18.98 6.01 0.601 0.618 10.9 0.601 100.0 0.618 10.9 1.8 AA 10 10 13.67 19.53 5.86 0.586 0.586 BB _10 --- ---- JQ__ 13.43 - - ~60__ 7.17 0.717 0.717 ------ Oucraii!Ol: MSD= Minimum Significant Difference Dunnett's MSD value: 0.0838 PMSD= Percent Minimum Significant Difference PMSD: 1!,3 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. IntiS;. Dunnett's MSD value: 0.0703 Lower PMSD bound determined by USEPA (lOth percentile) = 12%. PMSD: ll.l Upper PMSD bound detennined by USEPA (90th percentile)= 30%. Lower and upper PMSD bounds were determined from the I Oth and 90th percentile, respectively. of PMSD data from EPA's WET Interlaboratory Variability Study (USEP A. 200 Ia, USEP A. 2001 b). USEPA. 200 I a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-o l-G04 and EPA-821-B-01-GOS. US Environmental Protection Agency. Cincinnati, OH.
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Larval Fish Growth and Survival Test-7 Day Growth Start Dare: 2/10/2009 TestiD: PpFRCR Sample ID: TV A I Sequoyah Nuclear Plant, Outfall 10 I End Date: 2/17/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales prome1as Comments: Cone-% 1 2 3 4 D-Control 0.6110 0.6300 0.6100 0.6650 11.3 0.6420 0.7200 0.7290 0.7470 22.6 0.6640 0.7400 0.6360 0.6630 45.2 0.7160 0.6220 0.5160 0.5770 72.6 0.6420 0.5710 0.6390 0.6760 100 0.6260 0.6540 0.6470 0.6880 Transfmm: Untransformed !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6290 1.0000 0.6290 0.6100 0.6650 4.086 4 0.6714 1.0000 11.3 0.7095 1.1280 0.7095 0.6420 0.7470 6.537 4 -2.315 2.410 0.0838 0.6714 1.0000 22.6 0.6758 1.0743 0.6758 0.6360 0.7400 6.623 4 -1.345 2.410 0.0838 0.6714 1.0000 45.2 0.6078 0.9662 0.6078 0.5160 0.7160 13.860 4 0.611 2.410 0.0838 0.6312 0.9401 72.6 0.6320 1.0048 0.6320 0.5710 0.6760 6.961 4 -0.086 2.410 0.0838 0.6312 0.9401 100 0.6538 1.0393 0.6538 0.6260 0.6880 3.938 4 -0.712 2.410 0.0838 0.6312 0.9401 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98361433 0.884 0.19270246 0.87105609 Bartlett's Test indicates equal variances (p = 0.37) 5.42555952 15.0862722 Hypothesis Test (l-tai1, 0.05) NOEC LOEC CIJ_V TIJ MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.08379661 0.13322196 0.00540594 0.002417958 0.09521794 5, 18 Treatments vs D-Contro1 Linear Interpolation (200 Resamples) Point % so 95%CL(Exp) Skew IC05 41.450 ICIO >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqnl01_02-J0-09data-uv
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Larval Fish Growth and Survival Test* 7 Day Growth Start Date: 2/10/2009 Test ID: PpFRCR Sample ill: TV A I Sequoyah Nuclear Plant, Intake End Date: 2117/2009 LabiD: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% 2 3 4 D-Control 0.6110 0.6300 0.6100 0.6650 100 0.5670 0.6010 0.5860 0.7170 Transform: Untransformed 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6290 1.0000 0.6290 0.6100 0.6650 4.086 4 0.6290 1.0000 too 0.6178 0.9821 0.6178 0.5670 0.7170 10.945 4 0.311 1.943 0.0703 0.6178 0.9821 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.85388428 0.749 1.514357215 2.37378198 F-Test indicates equal variances (p = 0.15) 6.91965199 47.46722794 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.07027929 0.11173178 0.000253125 0.002616125 0.766281605 I, 6 Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 95%CL(Exp) Skew IC05 >100 ICIO >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqnl 01_02-1 0-09data-uv
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Concentration Control 11.3% 22.6% 45.2% 72.6% 100% 100% Intake
Page 5 of 6 Species: Pimephales promelas Date: O"Z.*tO*ot\ Client: TVA I Sequoyab Nuclear Plant- UV -treated 100% Intake
Page 6 of6 iETS
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Species: Pimephales promelas Date: _ __:O::...:'L:::...**....:.1~0.....:*D=-l]..J..__ Client: TVA I Sequoyah Nuclear Plant- UV-treated Concen- Parameter tration I I 11.3% I 22.6% .I I: 45.2% I 72.6% I 100% I I I 100% Intake I I 'ge 58 of98
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Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pH/Ion Meter Ana!ym Date analyzed I ~cz 0 ?j 0 j q Iodide reagent: Acid reagent: li--<T~f\~[_l_J..__c._l----1
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Sample ID Sample characteristics Note: All samples were analyzed in excess of EPA holding time (15 minutes) unless otherwise noted. L aboratory contraI standard: .< Reference standard True value (TV) Measured value (MV) %'ItS'.= j\1V I TV x 100
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Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.!0 mg!L Meter: Accumet Model AR25 pH/Ion Meter Analyst Date analyzed I ol /11-ML""-
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Acid reagent: Tl\ ~ J:/\~ 3~
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D L(J ,oo JC{q I Sample ID Sample characteristics I I I I I Note: All samples were analyzed in excess ofEPA recommended holding time ( 15 minutes) unless otherwisenoted. I L aboratory controI stan dard: Reference standard True value (TV) Measured value (MV) % RS = MV /TVx 100 number (mg/L) (mg/L) (acceptable range= 90 to UO%) I _It\.<'
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Reviewed by ~-....l£'-'-'~---=---l lage 60 of98 Date reviewed L--...::0_'2..:::::..---'-'\'2...._-_,d\,_,___,
Page l 0 Page _j_____ of_'l._ 'I& ~ ~ ~E-n-vlro_n_m-en-ta-1~-.s-tin-g-So-lut-Io-ns-.In<-. Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pH/Ion Meter Analyst Date analyzed I l Jg12? 01..-\ y- 09 Iodide reagent: Acid reagent IJNCZ..3 z. 1 Il\ll2.WID Calibration: Laborato control standard: Reference standard True value (TV) Measured value (MV) % RS =MY /TV X 100 (mg/L) (mg/L) (acceptable range= 90 to 110%) 0.50 Sample characteristics Sample ID Sample characteristics c.. o .ococ '!I'Z.B
'-O.oot"t'Z.
Laborato control standard: Reference standard Measured value (MY) % RS=MV /TVx 100 number (mg/L) (acceptable range= 90 to 110%) Reviewed by 11--J..~.{~,::Cv-:...;..,..-;---;:,---l Date reviewed o}~ {> j
Page_l..!.._L_I_ Page __1:_ of ...:L__
} Environmental Testing Solutions, Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pHil on Meter Analyst I ~ Iodide reagent: ~-'-:........-__::::......:;;::::------! Date analyzed C1.-I\.\.-O~ Acid reagent: ~
~--------~
Note: For samples with a residual chlorine of > 1.0 mg!L, the calibration range must be adjusted to bracket the chlorine levels of the samples. L aboratory control stan dard: Reference standard True value (TV) Measured value (MV) % RS = MV /TVx 100 number (mg/L) (mg!L) (acceptable range= 90 to llOo/o)
- Ct\JSSS1'1 0.50 0 ."{q~ qq,ln'/*
Sample characteristics SampleiD Sample characteristics Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. L aboratory control stan dard: Reference standard True value (TV) Measured value (MV) % RS = MV /TVx 100 number (mg!L) (mg!L) (acceptable range= 90 to 110%)
~~c:qq 0.50 o.S0-:6 IDO.lo'{,
Reviewed by I ~~~ Date reviewed t--o.,...'-'Z...'--14~.-6-=q---l age 62 of98
Page _q_,_____ Page _ _:___of ~ Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCOiL 113 3D J 1 Analyst ~..!:.Bsc.-.c::==-------1 Time initiated :1 Date analyzed ~. o'8 . oq Titrate samples to Time completed ===ilf-::s:o=======J~ ~ Titrant normality an d mu 1tip,rter determ natwn: pH of Normality pH= 4.50 S.U. Normality (N) of H2S04 pH Factor or Multiplier l I j Deionized Titrant check Begin End Total = (5 ml Na 2C03 x 0.05)/E = (N x 50000)/100 ml sample l water reference standard ml ml ml = 0.25/E =Nx500 !
=4.5 s.u. number number (E) (acceptable range= 0.0180- 0.0220) 5.~ :r~ f!. ,:n., :i). lSSI.R53 () .:, 1~.3 19,.0 o.o~o~ lo .Y. !
1"3\\l... e..crr~cii.,(\: D.u-7 0. '2>:: 0-3n\ \ L aboratory contra I stan dar d: Reference standard True value Sample Alkalinity (MY) % RS=MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCQ,/L) (acceptable range (mgCaCOYL) (ml) ml ml ml = 90 to 110%)
- rNSS ll2 'D :J. 100 100 \~.3 ~.-3 10.0 lc .4- tee"~lo>+ I e t:Fte B$1.. I o+crt.c Alkalinity %RPD=
Total Multiplier (mg CaC03/L) {(S- D) /[(S+D)/2)} x 100 ml (acceptable range=+/- 10%) lo.'-t s (cO\ I .~ lo:O D _L lt .I lR3 I M atrzx . spik e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mgCaCOYL) I .:C~S\QO::} (mg CaCOYL) 50 (ml) ltlD ml
~£).3 ml tll3 ml 11.0 IO !+ \10 I Sample alkalinity (B)
(mg CaC0 3/L) Measured spike value (MY) MV=A-B
% R=MV /SVx 100 (acceptable range
=75 to 125%)
(mg CaC03/L) I samp* e measurements: l.p?;, 4 C\40lc Sample volume Begin End Total Alkalinity I Sample number DZ-OS*CAP.., Sample ID ()')'\'\~ 1-hO (ml) too ml 39.?, ml
~-'+
ml lo. \ Multi(llier lD.Y. to3 (m~~: CaC03/L) 02-n-:J.oq -l .~~. . RY (.,.I lR .I I 1\1-DY .ua... S~u.._} i-!M:> ~1.5
~ 14.l# .3. i lP3 3;l D\*25-a:::\ M\4"\ 1-\.,o 14.to ~0.~ {p,O loJ-I ol*l-:t-0"1 Ol.l1-oct8 A
~
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(p.o {g;l. {£.).
~ 02-!i>-_QqQ I r>1.--b~6 ~
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3<g .\p H4.{c l.R .o _k.O tua. {o2. OZ.-03-o'1A SA-t:[~ -L-- o.o <1.r:l. ~ . . 1--- IOO lage 63 of 'lS Reviewed by: Date reviewed: I Ol-a:l ..tf?
Page \0 Page Ql of-'~>=-- Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Analyst Date amlyzed j'-----g W-0~-~ Titrate samples to Time initiated R Time completed ~~---~=::;;;..,------; pH= 4.50 S.U. Til171nr normallly .. . *.*
- I:
nata*-ination: pH of Normality N. mality (N) of H 2S04 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na 2 CU3 x-u~ ""' (N x 50000~ ml sample water reference standard ml ml ml - 0.25/E -==m 500
= 4.5S.U. number number (E) (acceptable range= 0.0180- 0.0220)
L ab ora ory contro stan dard: Reference standard True value Sample Alkalinity (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaC03/L) (ml) ml ml ml =90 to 110%)
.:r~s lR o-:r- 100 100 9.'1- ICJ.tp q,q lD .'4- JO~ lo?> rrzo Sample Alkalinity %RPD=
Sample Sample ID volume End Total Multiplier (mg CaCOJIL) {(S- D) /[(S+D)/2)} x 100 number (acceptable range,;,+/- 10%) teA: s
- 01. -b3--C9\ P.::> .:Sa..£T'Su.J Duplicate (B) D J J._
u atriX . spz'ke recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCDJ/L) (m!! CaCO)!L) (ml) ml ml ml J:~leO-:t- 50 100 B'l.l 1-B.O 13~ lc !t .~~~so) Sample alkalinity (B) Measured spike value (MV) % R=MV /SVx 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOJIL) ~
= 75 to 125%) -
\OD ~SSt--*~@___, n 117
. Ill ....U f l.lY1*
\f IJ samp, e measurements:
Sample volume Begin End Total Alkalinity Sample number Samole ID (ml) ml ml ml Multiolier (mg CaC03/L) i)j) sa-VSw I43.0'l Oz ..-o ":l-OCi }OD -:sol) ""~.'} C\.1- tt>.tt' lt>O OCJam-1.12- I (Xzoh n-)o..~ ~~2.0 ~1-- &st. 2.1~ Y.. I ts) ~ID 1.1. () Q tn.otlJ.~ mo_rshiL '"'*2.
-H ~\C'tl --15-e~ 1'1-.1- I.S I~
0'\ b'20~. bL. n"lozo~.o$ tl'i A- ~ e,"V) tlJA-Gt.Blrlh ~\~
-z.o .:21.'b
~?..t:;
~E..S ldf\.IJ
\ 1.&) [!">)
l<t,
'13 lA0'2.o~ o'-\ 'IJA ~r fbt"d"i/\D .BoO 015-0 o(CJ.C 4.0 ~
tJ1~2()3.01 foMwd ~ .Jq.o St.'} 5.+ sq fY1oloS.ol \ 1.- ,0,4.1-- 1"44.1 q~ C\CO Page 64
\)l)Q2o1-DZ.. ~ 3 _:...- %.1 Hcl.2- 5.\ _l--
53
** /U Reviewed by: Date reviewed: I 02.-0 'f- 0'1 ]
Page _1_1__ Page _ _ _of 3 Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCOiL Analyst I {%1.. Date analyzed t--O..::..C),-.-,-5-.-()G\--~ Time initiated ~LI.....:..~~2.:.=D=------1 Titrate samples to Time completed 154S"' L..!..=..;~-----l pH= 4.50 S.U. Titrant normality and mu tiplier determination: pH of Normality Normality (N) of H2S0 4 pH Factor or Multiplier I .] Deionized Titrant check Begin End Total = (5 ml Na2C03 x 0.05)/E = (Nx 50000)/ 100 ml5ample ; j water reference standard ml ml ml =0.25/E =Nx 500 i :1
=4.5 s.u. number number (E) (acceptable range= 0.0180- 0.0220)
~-Hp3 J:N~1-I J:"t\155loS3 0.1 I.X.o 11.'1 ().OaiO ID-5
&Ill C..rre.t..-t'ioo*. l:).o-7 0.1 i Laboratory contro I standard: 'i I
Reference standard True value Sample Alkalinity (MV) % RS=MV /TV X 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaC()JiL) (ml) ml ml ml =90 to 110%) I~SSl~ 100 100 1(~-0 ~~1.'-l* C\74- loS qq qqq. Sample Alkalinity %RPD= Sample ID volume End Total Multiplier (mg CaC03/L) {(S. D) /[(S+D)/2)} x 100 (acceptable range=+/- 10%) s 1¥-..\-hO 10.5
+ Duplicate (B)
.L ~.s -33.S' lu.O J,...
D M,atrtx spr'k e recovery:
,Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L)
(mg CaC03/L) (ml) ml ml ml IJ\1S.S.I.psY So \DD ~1.5 -3~.0 lb-'5 to.S l\b Sample alkalinity (B) Measured spike value (MV) % R=MV /SVx 100 (mg CaCO]!L) MV=A-B (acceptable range (mg CaCO]!L) = 75 to 125%)
~3 11 q4~
s,amp,l e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Mult!J>iier (me CaCO]!L) D2-lb-Cf1 rn \1s.t-ho toO ~1..0 ~:?,.!\ 5_9_ lOS ~2. Dt- J\~*OCJ 6sw t-\..0 _L-0*0
,.,""...ti 6.1 3., \ .~(33) flz~6~-oCJ I 11\\\~ '"ho 3.1 CJ.'Z. 4>.( ~~
Ln.J I o
- 001 A .,J q.l, IS.t 5.Cl &z, Al~ o3-oOJB rn ~s v u ~ 115'.1
~1.0 f!61.o 5 .Ci lc/2, h7 .()9-00
- 62. -10*0'1 8 (YlOZ II. I (
J I
-(fA i<u::ootMflUY i 2.
-.3 Ol~
.21.0 J+.o
~-~ l3q.t
.)1.o
&<.~
L.O 5.g 5.\c.{i l62 (Q3 ln\
2;(;) * ::tte"'"~~ ~J
()~02..11./0 J en~ P-~tJC>\ J_ 3<g~ 2A.I 0:1- ~ ~-~ ~2'1 Page 65 of98 Reviewed by: Date reviewed: I 02--IS . . lJ~
9J Page 11> e* Page ?? of 3
~ -=--- *'!\ _*
- tJ#
-----=---
Environm~ntal Testing Solutions. Inc:. Alkalinity (SM 2320B) Matrix: Water, RL = 1.0 mg CaCOiL Analyst Date analyzed
~I---~85~L:::.----;
a;1. *1'5 *CCf Titrate samples to Time initiated I '-. . . . . . . . ___ . *I Time completed ,__ _ _ _~..,--~=--->. pH= 4.50 S.U. itrant normality and multtp1Zer t r determmatwn: pH of Normality Normality (N) of H2S04 pH Factor or Multiplier Deionized Titrant check Begin End Total - (5 ml Na1<..v3 ~ ".n"\fli' -' (N x 5000q)/IOO ml sample water reference standard ml ml ml - 0.25/E -~500
=4.5 s.u. number number on (acceptable range= 0.0180. 0.0220)
L aboratory contro1stan dard: Reference standard True value Sample Alkalinity (MV) % RS =MV /TV X 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJiL) (ml) ml ml ml =90 to 110%) INS-!> tp61 100 100 3q.l *1-~3 9.2 I(}.S l11- "ic"l-o Alkalinity %RPD= Begin End Total Multiplier (mg CaCOJIL) {(S- D) /[(S+D)/2)} x 100 ml ml ml (acceptable range= :1: 10%) 1o.s s ':}o D.O 4..1' (.., .-:r-Dup lie ate (B) D
.J. ~ 135"" U*g ..L '11 Mit.a rtx spz'ke recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) _(JIIg_ CaCOJIL) (ml) ml ml ml 1N SS.lo6~ 5o too lQ.i- lf02- ll.S 1o.S 120 Sample alkalinity (B) Measured spike value (MV) % R = MV I SV :x: 100 (mg CaCOJIL) MV=A-B (acceptable range
.,, (_m_g_ CaCOJ/L)
>-tq
=75 to 125%)
q~ samp11e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier ( mg CaCOJIL) oqb 2-t11-0J T'./'A~..J I lot> 'q ,?..- ~5.2 t:J ,0 IO.S Ia{ ()Cfo2-l~b5 .1 3 ~.5.'2. ld:23_ 1.1 I ~(Qs)
~
{) ~ b2 flCIJ .0~ 1\JA~~\M I ~.:, '6'\,_:,i '1-.o 1-4 V~D2/ l 15 J '2. ?ft.3 1-fJL.'L li-'1 t-~ D1b2J?:>. Olo ~ ..3 0.0 \1/.Cj LR.~ 'l~ D1b2Jn.D ) .rf A ~6&~ 0\J i t.g,q ~~.~ tR-~ q.) {)tjo 2 IL t lJ l 2 1~.1- ~~c.\p lt-~ ~ f:lt-) Oqoz-13.1/5 J 3 ~0-I.P l.J:J .~ lu '1-u __,.__ *-:tz+ 0007. (fl. 6'k 1l'A~SG.tJ\IJ\vv t 1~.3
.;)1.~ '1.0 Reviewed by: Date reviewed: I 0'2 -IS ~U1
I Page _1..:::2.=---- Page 3 of_3""'--- I Alkalinity I (SM 2320 B) Matri.'c Water, RL = 1.0 rng CaCOiL
~-B'"'(,""'(..""------J I ...,~=------i I Analyst Date analyzed 0~ . 16. D4 Titrate samples to Time initiated Time completed 1----'..
pH= 4.50 S.U.
- r det ermmatwn:
I r:1trant norma lity an d multtpuer pH?f Deionized T1trant Normality check. U<'J;;III .... "'* ** Normality (N) of H 2S0 4
*(5 ml Na 2C0 3 x 0.05)/E pH Factor or Multiplier (Nx 50000)/100 ml sample water reference standard ml ml ml u.... _,,~ - Nx 500 I -4.5 s.u. number number (E) (acceptable range 0.0180- 0.0220) lk
'?
I L ab oratory controI st an d ard : Reference standard True value (TV) Sample volume Alkalinity (MY) % RS = MV I TV x 100 (acceptable range number Begin End Total Multiplier (mg CaCO:YL) (mg CaC03/L) =90 to 110%) I (ml) ml ml ml
\J'JSSlP5~ 100 100
?A.:; ~3!t 'LI IO.$ Cf(g Cill~
I Multiplier Alkalinity (mg CaCOJIL)
%RPD=
{(S- D) /[(S+D)/21} x 100 (acceptable range=+/- 10%) I 10.5 s D I M at.rrx sp1'k e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) I h~~S.lt>sY (mg CaC03/L) 50 (ml) iOO ml {g .\.p ml 1ro.t ml I0.-5" l~D
"* "5 I Sample alkalinity (B)
(mg CaCO;VL) Measured spike value (MY) MV=A-B
% R =MY I SV x 100 (acceptable range
=75 to 125%)
(mg CaCOy'L) 1-, I Sample I measurements:
~q q~~
Sample volume Begin End Total Alkalinity I Sample number () 16Z/I. fb Sample ID
-rvA-~~a:;;:Ku\J z lO D (ml) ml
! '6.\
ml
~5.0 ml le-4 Multiplier It:~.~
(ml! CaCOJIL)
"?.;:t
{Yi D2 ll .0 J 6Z.m ~1.5'0 ~~.o 2tt :' I !f t;J.' &q I ()r)\ ()~DZI z_oL tiD O'D\ l~lP4 kl/5 1.1 lz 1
~~3)
O"JDl.ll.t> 3 C02 ~~.s -~-~ q z (2." ~10 1 D'ib£.12. .t>~ UD002. :..- ~i.3 ~~.\R 1.~ L11 .:)-:J-61o'JD7.11 1-k.rr~ ~ Jo. I zs 3ttu 41.-:} 3.\ ILl' l?.o 0462/D,() i \ 2. 41.'1- 45.0 33 JA' l~o DqD21l {)~ .J 3 . IJ.f-5.0 "-llri.1 ~~.1-ILi~ ~lt,O 02.-lQ.()q S/}liS W too e,Ji£ 1(\~ ~.0 -~ '6q. tage 67 of 98 Reviewed by: Date reviewed: I OZ-1'5 -o'1
I
- 11
~
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~ Page ...;l.'~ of_,.;l.=--
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. } E-nvf-ro-nm-e-nta-ITe-sa-*ng-So-lut-ion-s,-lnc.
Total Hardness (SM 2340 C) I ~~Pi>""""""l-'------i RL = 1.0 mg CaC03/L Time initiated 11-..;.I.:.D..:::O..;.lP_ _ _ _--t I Date analyzed Analyst
~ . 0'5 . cq 11 15"'
Time completed .______:::..._ ___ ___. T.itrant normality and multiplier determination: I Titrant* reference number Normality check standard number Begin ml End ml Total ml (E) Normality (N) of EDTA
= 0.2fE (acceptable range= 0.0180 - 0.0220) pH Factor or Multiplier
= (N x 50000)/ 50 ml sample
=Nx 1000 tftJ~o<& TN'SS 6--::Jl o.o D.o.Qo cQo .o I
IO.D [0.0 Lb a oratory controI stan dard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 I number (TV) (mg CaCOJIL) volume (ml) 50 Begin ml End ml Total ml Multiplier (mg CaC03/L) (acceptable range
=90 to 110%)
X t.SSS (Qb 4> 40 lD.o 1~.0 .;?.o ~0.0 J-fo.o lt>o~ I D~r"shot c,olcr I Begin ml 1.;u) End n:o ml s IDO I D _L 11-'ru . 1-5 1-J-5 .L CJo
.;/1.'5 o\lu ,J ff-~ o/o.o q2. ~ ..;;tatlo Mat. rtx spt'ke recovery:
I Reference s'tandard number Spike value (SV) (mg CaC03/L) Sample volume (ml) Begin ml End ml Total ml Multiplier Spike hardness (A) (mg CaCOJIL)
- r: t>J'Os l.Q 5le 4D loo I tu.5
~l.S' ,;j'3.0 C}o,O 130 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range I CJJ.
(m2 CaCOJIL) 3'6
= 75 to 125%)
Cl'SO'Lo samp.el measurements: I Sample number Sample ID Sample volume (ml) Begin ml End ml Total ml Multiplier
*Hardness (mg CaCOJIL)
TV=ND Blank :5P .;7t>.O I 1'>2
- bS *Cf\ 6 (should be= 0 mg CaCO,/L)
(Y)tls rlw fG.Oest.. J.~.o J.Y>.o on.o o.o 139..5 1-t.S
~~ "->0 qi:)
_b_ '1. -D4 *DC\ .J &l.5' ?;?.o 4.5 l1o I 02-0\-0Ci SsW :n.o 39.1 ll .t.+;l OI.Z'&-0'1 mHs .. u.o 1.~'1.1 ~g,g 4.-:t- C\1.)- I Dl-2-=t-DA~ {) 1-2.1 -cctB 43$ ~~.~ o.o --t.cs
'4-.S J.t.S 90 qo I DZ-b~-CG Ol-O.b-a4A A
. 4-.S' cu
<1.1 1,~.~
I-A-J.. v 4$ Dt2 ClLP rage 68 0 1 1~o.w-Hz-. WPsn~llY' v 16.£1 V1.'1-- 5$ -- \~0 Note: If >!Sml of titrant is used, sample must be diluted. Reviewed by: Date reviewed I 02-D6-oC)
*9 Page ....f6_;"""--
;@)
~
~
,sf;! Environmental Testing Solutions. Inc.
Page CJ- of----"",;1._,___ Total Hardness (SM 2340 C) RL = 1.0 mg CaC03/L Analyst ~~e£:G.=~----t Time initiated I==-==== ._ Date analyzed OOl. _D~- c:fl Time completed '-------.....J T.itrant normar'tty and multiplier determination: Titrant Normality check .... _li'.nrl Total Normality (N) ofEDTA pH Factor or Multiplier reference standard ml ml ml , 02/E (Nx 50000)/ 50 ml sample number number (E) (acceptable range= 0.0180- O.u.:.:u Nx 1000 L aboratory control standard: Reference standard True value Sample Hardness (MY) % RS=MV /TV X 100 number (TV) volume Begin End Total Multiplier (mg CaCOYL) (acceptable range (mgCaCOYL) {ml) ml ml ml =90 to 110%)
- r )0 '&Slo5~ 40 50 C)s-o?.o I 'l :).- oh~ I.Cf ~1:>.0 .~~
Hardness %RPD= Multiplier {mg CaCOJIL) {(S- D) /((S+D)/2]} X 100 s 2.o.t Duplicate (B) D J J_
.5 ~-'+
Miatrrx . sp1'k e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mgCaCO)!L) (mg CaCOJIL) (ml) ml ml ml
;:c wss ~'0 (R 1--{o 5D ;?4.1 .;?8~ 4.3 .;;>o .o %~
Sample hardness (B) Measured spike value (MV) % R=MV /SV X 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%)
.1-f~ 3~ '1C:St:>Lo samp.l e measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO:JL) TV ,,~.'r) Rl::mlr I... (should be= 0 mg CaC03/L) I riMn..o~ .ot.o hTA ~ R.\JiJ) joo ~'?>-'+ :3o.o l.lP 2o.o ~
~~~*OS
~ 8E:w~ u_
~11nz. Jo &H- ~~-~ (p_tt l-5) lP'-fo oqo '1.. o ~ *(")'-' 'rf{A S)Jt: PO\....I{j\.ti\i. 10 o.o (p.t.j- \Q.tf I(!=;) l~.lfo bt1ol aq.o l Scu::h.J/l CJ ~~0 loo Ia.~ 1~.3 5/5 /10
{)t:}OZO~.OJ ft:N.uxn--;{ l 1'3. ?> ~o.) ~-J.. J.j.L}- 1()205.01 .~eze~vjr"' z. .:Jo.r) ~4-.1 _3. \o -:/;1.. b'11J2o+.oz... 3 h ..14.1 lon-.5" ~14-
- (o&
./
Page 6 ~ ot'9H Note: If >15m! oftitrant is used, sample must be diluted. Reviewed by: ltll:..V'-- L-'-'-----.....J Date reviewed I oz-oB~oq
I Page _-4..:._'1-=---- Page --'-_of 3 I { ) Environmental Testing Sclutlons, Inc. I Analyst I J!,<;(., Total Hardness (SM 2340 C) RL = 1. 0 mg CaC03/L Time initiated ~~50 I Date analyzed t-DJ.-=-""',:::..____4_--t 5 0 Time completed lll-1 0
~-..:_:_:...;:;.. __
f--...:...:.;...:;:;:..=.------t
~*ztrant normality and multiplier determination:
I Titrant reference number Normality check standard number Begin ml End ml Total ml (E) Normality (N) of EDTA
= 0.2/E (acceptable range= 0.0180- 0.0220) pH Factor or Multiplier
= (N x 50000)/ 50 ml sample
= Nx 1000
.:rt-Je.3o~ !N'SS 5-:::t \ 0.0 Jo.o 10.0 0 .0.:{00 ;(0.0 I L ab oratory control stan dard:
Reference standard True value Sample Hardness (MY) % RS =MY I TV x 100 I number (TV) (mg CaCOJIL)
*volume (ml) 50 Begin ml End ml Total ml Multiplier (mg CaCOJIL) (acceptable range
=90 to 110%)
rt..>sS t.c5~.p 40 iu.o ld..O J?,O ~0.0 4o.D loo-:TJ,o I Hardness %RPD= I Begin End Total Multiplier (mg CaC(h/L) {(S. D) /[(S+D)/2]} X 100 ml ml ml s qi 1~.0 lv.Cf ~1).\J D
~
Duplicate (B) I .l- f!Q, q ~l.l! 4.1- l- C/4 Matrix spike recovery: lYH S0'1- *\ I Reference standard number Spike value (SV) (mg CaCOJIL) Sample volume (ml) Begin ml End ml Total ml Multiplier Spike hardness (A) (mg CaCOJIL)
.:J"t.JSS\.4l~ £.,.. J.fo 5o ~~~ altO (p .J.t Jo.o f3D I Sample hardness (B) Measured spike value (MV) %R=MV /SVx 100 (mg CaCOJIL) MV=A-B (acceptable range I '3'3 (mg CaCOJIL)
;{J.
= 75 to 125%)
loSe-to samplel measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC03/L) Blank TV=ND (should be= 0 mg CaC03/L) so ~I.{.? ~*-l; ~t>.D tvO OZ.;I~*al fl'll+~ 1:12() !..;(11.\/J ;lv .o ~H- BB Dz.~l ~-Oe.t (;, -s.u.J 1-h.O ~~ 3o~ ~~ 4t3 I ()2.-0t-a:f m'+s \4z1J 3o.r+ 35,0 4-l-:> '12.. 02-- H). V1 (\- .J. 3$.0 39-~ 4-.rJ qo I b ~-03.1118 01- -o+uCJ ffilts ll\J I I z 3q.~ .q.o!t,O
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- Page S:>
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_.J Environmental Testing SolutiOns, Inc. Analyst I '6S<... Date analyzed 0 ~ _ l5. D'J itrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier refereil"-' d~ntl,.,.tl ml ml ml . 0.2/E (N x 50000)/ 50 ml sample number number I acceptable range 0.0180- 0.0220) Nx 1000 L aboratory controI stan d ard: Reference standard True value Sample Hardness (MV) % RS= MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaC031'L) (ml) ml ml ml = 90 to 110%) Xt-.:6c; ~ olP 40 50 co ,{p 1{).'1* ~.\ ~o.o rt~ ID501.o Sample Hardness %RPD= volume Begin End Total Multiplier (mg CaC03/L) {(S- D) f((S+D)/2]) x 100 ml ml ml
-SqtJ s
\t> :-:} 15.0 If.~ ~o.o SIP Duplicate (B) D 15.0 tCi;7.. 1-j.,'}_ j_ 84 M,at. TIX spt'ke recovery:
Reference standard Spike valu.e Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaC031'L) (mg CaC031'L) (ml) ml ml ml
- l:i'Y5S.l.il5LP .t..to 5o 15.0 ~1-1 Lc>.l ~0.0 1'20 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaC031'L) = 75 to 125%)
CC'f 3l.? qoc-to Sample I measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC03/L} TV ND Blank ~
~.
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Page 71 of 9R IvA 5& \.) tuivu ' .J. B.5 1).$ 4.:) - \?" Note: if >15m! of titrant is used, sample must be diluted. Reviewed by: Date reviewed I 02~ 16'-0't
Page -=5:....:.1_ _ Page 3 of_,3~-
.,) Environmental Testing Solutions. Inc.
Analyst I Date analyzed 1--"W.=*-,S--0-9-~ e,SL.- Titrant normality and multiplier determination: Titrant Normality check Begin End Total "Normality (N) of EDTA pH Factor or Multiplier refere..-a.. ohnilar-d ml ml ml 0.2/E (N x 50000)/50 ml sample number number lE} \~H'I '0.0180. 0.0220) Nx 1000 N L aboratorv control standard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaC03/L) (ml) ml ml ml =90 to 110%) 40 50 l1H1
;J::rJSSl:!o~ J~.<3 1-fc:;\ Jo5VZ.C
~-' ~() .Q Hardness %RPD=
Total Multiplier (mg CaCOJIL) {(S- D) /[(S+D)/2]} x 100 ml
<Jo.o s 8<;
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Duplicate (B) D j ltt.z. ;;)J~ 4.'2.. J_ Btf Miatrlx sp,ike recovery: Reference standard Spike value Sample Spike har~ness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (m2 CaCOJIL) (ml) ml ml ml
]:NSS(e'Olo 4o 5t> j{.j. 2- ~It t.v.2. Olo.o I .;~o Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%)
COY. 3lP go~ Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multi}>lier lm11. CaCOJIL) TV=ND Blank ll'-
\SfiUUlU DC m~ ~aLVJ/
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- Date revtewed ~.-1...:0:::.1.=--l:...:"5::...~.:::.()_:~--~
Sequoyah Nuclear Plant Biomonitoring February 10 - 17, 2009 Appendix D Reference Toxicant Test and Control Chart Page 73 of98
~
Pimephales promelas Chronic Reference Toxicant Control Chart
~
Organism Source: Aquatox, Inc.
~ Environmental Testing Solutions, Inc.
USEPA Control Limits (+/- 2 Standard Deviations) 1.0 0.8 0.6 0.4 1.2 u
~
1.0 USEPA Warning and Control Limits (75th and 9dh Percentile CVs)
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r-1.2 Laboratory Warning and Control Limits (1 dh and 251h Percentile CVs) 1.0 0.8 ..... ******* 0.6 -------**-**- 0.4 Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC25 ) Warning Limits (mean IC 25 +/- S AIO or SA75) Control Limits (mean IC 25 +/- SA.25' SA 90 , or 2 Standard Deviations) Page
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Pimephales promelas Chronic Reference Toxicant Control Chart State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-dayiC,. CT s Control Limits SA. II Warning Limits SA.2S Control Limits SA.7S Warning Limits sA... Control Limits CV (g!LKCI) (g!LKCI) CT-2S CT+2S CT- SA.Jo CT + SA.IO CT- SA.>S CT + SA.2S CT- SA.75 CT + SA.75 CT- SA.,o CT + Suo 1 04-15..08 0.60 2 05..06-08 0.62 0.61 0.01 0.59 0.63 0.07 0.54 0.68 0.13 0.48 0.74 0.23 0.38 0.84 0.27 0.34 0.88 0.01 05-20..08 0.66 0.63 0.03 0.57 0.68 0.08 0.55 0.70 0.13 0.49 0.76 0.24 0.39 0.86 0.28 0.34 0.91 0.04 4 06-03..08 0.64 0.63 O.o2 0.58 0.68 0.08 0.55 0.70 0.13 0.50 0.76 0.24 0.39 0.87 0.28 0.35 0.91 0.04 5 06-10..08 0.76 0.65 0.06 0.53 0.78 0.08 0.58 0.73 0.14 0.52 0.79 0.25 0.41 0.90 0.29 0.36 0.95 0.09 6 08-05..08 0.69 0.66 0.06 0.55 0.77 0.08 0.58 0.74 0.14 0.52 0.80 0.25 0.41 0.91 0.30 0.36 0.96 0.08 7 08-12..08 0.76 0.67 0.06 0.55 0.80 0.08 0.59 0.75 0.14 0.53 0.82 0.26 0.42 0.93 0.30 0.37 0.98 0.09 8 09..09-08 0.79 0.69 0.07 0.55 0.83 0.08 0.61 0.77 0.14 0.54 0.83 0.26 0.43 0.95 0.31 0.38 1.00 0.10 9 09-11-08 0.59 0.68 0.08 0.53 0.83 0.08 0.60 0.76 0.14 0.54 0.82 0.26 0.42 0.94 0.30 0.37 0.98 O.ll 10 10..07..08 0.57 0.67 0.08 0.51 0.82 0.08 0.59 0.75 0.14 0.53 0.81 0.25 0.41 0.92 0.30 0.37 0.97 0.12 II 10-14..08 0.63 0.66 0.07 0.51 0.81 0.08 0.58 0.74 0.14 0.52 0.80 0.25 0.41 0.92 0.30 0.37 0.96 0.11 12 10-28-08 0.79 0.67 0.08 0.52 0.83 0.08 0.59 0.76 0.14 0.53 0.82 0.26 0.42 0.93 0.30 0.37 0.98 0.12 13 11-04-08 0.67 0.67 0.08 0.52 0.83 0.08 0.59 0.76 0.14 0.53 0.82 0.26 0.42 0.93 0.30 037 0.98 0.11 14 12..09-08 0.69 0.68 0.07 0.53 0.82 0.08 0.59 0.76 0.14 0.53 0.82 0.26 0.42 0.93 0.30 0.37 0.98 0.11 15 01-06-09 0.76 0.68 0.07 0.53 0.83 0.08 0.60 0.76 0.14 0.54 0.82 0.26 0.42 0.94 0.31 0.37 0.99 0.11 16 01-13-09 0.82 0.69 0.08 0.53 0.85 0.08 0.61 0.77 0.14 0.54 0.83 0.26 0.43 0.95 0.31 0.38 100 0.11 17 01-15-09 0.77 0.69 0.08 0.54 0.85 0.08 0.61 0.78 0.15 0.55 0.84 0.26 0.43 0.96 0.31 0.38 101 0.11 18 01-21-09 0.78 0.70 0.08 0.54 0.86 0.08 0.62 0.78 0.15 0.55 0.85 0.27 0.43 0.97 0.31 0.38 101 0.11 19 02-03-09 0.79 0.70 0.08 0.54 0.86 0.08 0.62 0.79 0.15 0.56 0.85 0.27 0.44 0.97 0.32 0.39 102 0.11 20 02-10..09 0.83 0.71 0.08 0.55 0.88 0.09 0.62 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 103 0.12 Note: 1-d IC,. = 7-day 25% inhibition concentration. An estimation of tbe concentration of potassiwn chloride that would cause a 25% reduction in Pimephales growth for the test population. CT = Central tendency (mean IC..S). S = Standard deviation of the IC 25 values. Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. Suo= Standard deviation corresponding to the lOth percentile CV. (SA 10 = 0.12) SA.,.= Standard deviation corresponding to the 25th percentile CV. (SA. 25 = 0.21) USEPA Control and Warning Limits sA.,= Standard deviation corresponding to the 75th percentile cv. ( SA7S = 0.38) SA.9o = Standard deviation corresponding to the 90th percentile CV. (SA.90 = 0.45) CV = Coefficient of variation of the IC 25 values. USEPA 2000. Understonding and A<:counting fur Method Voriability in Wbole Effluent Toxicity Applications Under tbe National PolluW!t Discharge Eliminalion Program. EPA-833-R.(){)-003. US Envirurunental Protection Agency, Cincinnati, OH. Organjsms obtainedfrom A quatox. Inc. atox02*1 0-09
- Precision of Endpoint Measurements
~ ~
ETS Environmental Testing Solutions, Inc. Pimephales promelas Chronic Reference Toxicant Data Control Control Mean Test number Test date Survival Growth CT cv CT MSD PMSD CT for Control Growth for Control (%) (mg!Iarvae) (%) (%) forPMSD(%) (mg!Iarvae) Growth cv (%) 04-15-08 100 0.898 9.1 0.08 9.1 2 05-06-08 100 0.857 0.877 4.4 6.7 0.16 18.2 13.7 3 05-20-08 100 0.844 0.866 19.2 10.9 0.16 18.8 15.4 4 06-03-08 100 0.918 0.879 6.1 9.7 0.09 9.4 13.9 5 06-10-08 97.5 0.724 0.848 10.9 9.9 0.11 15.7 14.3 6 08-05-08 100 0.854 0.849 15.3 10.8 0.13 15.0 14.4 7 08-12-08 100 0.674 0.824 5.3 10.0 0.07 9.8 13.7 8 09-09-08 100 0.710 0.810 11.1 10.2 0.12 17.3 14.2 9 09-11-08 100 0.824 0.811 11.1 10.3 0.11 12.9 14.0 10 10-07-08 100 0.788 0.809 1.8 9.4 0.10 12.9 13.9 11 10-14-08 100 0.740 0.803 3.2 8.9 0.05 7.2 13.3 12 10-28-08 100 0.586 0.784 6.5 8.7 0.10 17.8 13.7 13 11-04-08 97.5 0.643 0.774 2.5 8.2 0.12 18.7 14.1 14 12-09-08 100 0.522 0.756 2.4 7.8 0.05 10.0 13.8 15 01-06-09 100 0.644 0.748 3.2 7.5 0.09 13.3 13.7 16 01-13-09 97.5 0.556 0.736 9.7 7.6 0.06 11.2 13.6 17 01-15-09 100 0.707 0.734 6.5 7.5 0.09 13.3 13.6 18 01-21-09 97.5 0.594 0.727 4.9 7.4 0.07 11.5 13.5 19 02-03-09 100 0.671 0.724 8.4 7.4 0.06 9.6 13.3 20 02-10-09 100 0.591 0.717 5.1 7.3 0.09 15.7 13.4 Note: CV = Coefficient of variation for control growth. Lower CV bound detennined by US EPA (1Oth percentile)= 3.5%. Upper CV bound determined by USEPA (90th percentile)= 200/o MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10m percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile)= 30%. CT =Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200la, 200Ib. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtained from Aquatox, Inc. atox02-l 0-09 Page 76 of98
*** Pimephales promelas Chronic Reference Toxicant Control Chart
~ Precision of Endpoint Measurements
,_) Environmental Testing Solutions, Inc. Organism Source: Aquatox, Inc.
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Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)
Page 1 of 5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas I PpKClCR Test Number: 110 I Dilution preparation information: KCl CHM number: (,\-\ 11 t.t 01-Comments: I Stock preparation: 50 g KCIIL: Dissolve 50 g KCI in 1-L Deionized water Dilution prep (mg!L) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 I Diluent volume (mL) Total volume (mL) 991 1000 988 1000 985 1000 982 1000 979 1000 I Test orKanism information: Organism age: 1'\. So l-tt\uitS OL.b Test information: Randomizing template: .f'IJ~ fL.e_
*I Date and times organisms Incubator number and 01.* 0 "'. 04\ ll.oO 3E:
were born between: shelf location: Organism source: A"TM e,A\(.1'\ ~P ll'l* ri\*CA Artemia lot number: ehtw/10
";;"!* Transfer bowl information: pH= SU Temperature= oc Total drying time: "2.~* ~<HLI-5_
1.b?... '2"f**3 Date I Time in: 6.1.*i1*1l'i lfi_Q Average transfer volume: D. 1~8 4 .-J).. Date I Time out: uv1Ht\ 1ILQ_ Oven temperature: too* c. I' Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, MHS Analyst feeding feeding time batch used I
~***'" time
. 0 2
il 3 4
Page 2 of5 I Species: Pimephales promelas PpKCICR Test Number: 11 0 I Survival and Growth Data Day Control 450 m~:KCVL 600 m~ KCl/L I 0 A B c D E F G H I J K L
/0 10 IT; IO /Q /fJ IO to 10 10 10 10 I 1 IO 10 (0 '0 IO 10 10 10 ID 10 (0 /0 2 10 IO /0 10 10 10 10 10 I 3
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.Average Percent weight per reduction initial from control o.sq \ o.~~'S q.~1 o.s~4 4.17.
number of (%) larvae (ml!) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: L Comments: 79 of98
I ** Page 3 of 5 I J.t
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=A-B "i.OD '$. ~~ :>.~1.. $.11.\ q,,~ ~."l% ~.0~ l.o.\'% 1.11 \. ~\ 2..11.\ -z.o3 Weight per initial number l.~ ,-"
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* . == 1;IDUSually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed: ~
~ ~
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** Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control
~
"0:1:!? Verification of Data Entry, Calculations, and Statistical Analyses 0 Environmental Testing Solutions, Inc.
Test number: Test dates:
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PpKCICR 11170 February 16-17, 2009
'-""I coo...,tratioa CroWL llcpli- Ialtialaullll><rof Fiaalaumber of A ~ Pu wcig~t (mgj B =Pas + Larv1e La.., .. ll'<igjlt (rna) Wdglu I So"'ivi*K Mcuwciglll/ Coeftlc:ie*t ofvariadoo Wcigllt /laitial aumbc:r Mcln sunival Meaa Might/ laitlal Codli<ieor ol Perceat red.c1ioa rrom KO) 1...... larvae wcight (mg) *A-B ......... olWv&e (ma) Su..,..iviag umber of (M-.~..... ~
__._..,.._..,(%) oflarne (mgj (Y*) **mber of larvae var:ialiol t*!.) COitroJ W*) larvae (mg) (mg) A 10 10 14.09 19.58 5.49 0.549 0.549 B 10 10 15.03 21.15 6.12 0.612 0.612 Control 0.591 5.1 100.0 0.591 5.1 Not applicable c 10 10 13.94 19.85 5.91 0.591 0.591 D 10 10 13.79 19.92 6.13 0.613 0.613 E 10 10 14.26 19.16 4.90 0.490 0.490 F 10 10 14.46 20.28 5.82 0.582 0.582 450 0.548 9.9 97.5 0.535 12.9 9.6 G 10 9 13.83 18.46 4.63 0.514 0.463 H 10 10 14.12 20.16 6.04 0.604 0.604 [ 10 10 13.87 19.29 5.42 0.542 0.542 600 J 10 10 14.34 20.36 6.02 0.602 0.564 4.7 0.602 100.0 0.564 4.7 4.7 K 10 10 13.49 19.09 5.60 0.560 0.560 L 10 10 14.19 19.69 5.50 0.550 0.550 M 10 8 13.44 17.44 4.00 0.500 0.400 N 10 10 14.14 19.87 5.73 0.573 0.573 750 0.545 6.5 95.0 0.520 15.8 12.1 0 10 10 13.86 19.18 5.32 0.532 0.532 p 10 10 13.46 19.20 5.74 0.574 0.574 0 10 7 14.98 19.11 4.13 0.590 0.413 R 10 5 14.06 17.34 3.28 0.656 0.328 900 0.625 8.3 60.0 0.374 11.7 36.8 s 10 6 14.93 19.02 4.09 0.682 0.409 T 10 6 15.51 18.95 3.44 0.573 0.344 u 10 2 13.36 14.53 1.17 0.585 0.117 1050 v 10 3 14.08 15.89 1.81 0.603 0.645 9.4 0.181 27.5 0.179 24.3 69.8 w 10 3 12.30 14.44 2.14 0.713 0.214 X 10 3 14.75 16.78 2.03 0.677 0.203 Dunnett's MSD value: 0.0926 MSD= Minimum Significant Difference PMSD: !5.7 PMSD= Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. LowerPMSD bound determined by USEPA (loth percentile) = 12%. Upper PMSD bound deternrined by USEPA (9oth percentile)= 30"/o. Lower and upper PMSD bounds were detennined from the I oth and 9oth percentile. respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200 1a; USEPA, 200 I b). USEPA 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods. Volwnes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Ageocy, Cincinnati, OH. Organisms obtained/rom Aquatox. Inc. atox02-l 0-09
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Larval Fish Growth and Survival Test-7 Day Survival Start Date: 2/10/2009 TestiD: PpKClCR Sample ID: REF- Ref Toxicant End Date: 2/17/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR.-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mgiL 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 0.9000 1.0000 600 1.0000 1.0000 1.0000 1.0000 750 0.8000 1.0000 1.0000 1.0000 900 0.7000 0.5000 0.6000 0.6000 1050 0.2000 0.3000 0.3000 0.3000 Transform: Arcsin Square Root Rank I- Tailed Number Total Conc-mgiL Mean N-Mean Mean Min Max CV% N Sl!f11__ __ Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 450 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 16.00 10.00 1 40 600 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 750 0.9500 0.9500 1.3358 J.l071 1.4120 Il.411 4 16.00 10.00 2 40
*900 o.6000 0.6000 0.8872 0.7854 0.9912 9.469 4 10.00 10.00 16 40
*1050 0.2750 0.2750 0.5506 0.4636 0.5796 10.532 4 10.00 10.00 29 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) 0.83137703 0.884 -1.5887618 3.41097967 Equality of variance cannot be confirmed Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 750 900 821.583836 Treatments vs D-Control Trinuned Speannan*Karber Trim Level EC50 95%CL 0.0%
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Larval Fish Growth and Survival Test-7 Dar Growth Start Date: 2/10/2009 TestiD: PpKCICR SampleiD: REF-Ref Toxicant End Date: 2/17/2009 LabiD: ETS-Envir. Testing Sol. Sample Type: KCL-Potassiwn chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pirnephales promelas Comments: Conc-mg/L l 2 3 4 D-Control 0.5490 0.6120 0.5910 0.6130 450 0.4900 0.5820 0.4630 0.6040 600 0.5420 0.6020 0.5600 0.5500 750 OAOOO 0.5730 0.5320 0.5740 900 0.4130 0.3280 0.4090 0.3440 1050 0.1170 0.1810 0.2140 0.2030 Transform: Untransformed 1-Tailed Isotonic Conc-m~ Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5913 1.0000 0.5913 0.5490 0.6130 5.063 4 0.5913 1.0000 450 0.5348 0.9044 0.5348 0.4630 0.6040 12.856 4 1.397 2.290 0.0926 0.5491 0.9288 600 0.5635 0.9531 0.5635 0.5420 0.6020 4.739 4 0.686 2.290 0.0926 0.5491 0.9288 750 0.5198 0.8791 0.5198 OAOOO 0.5740 15.815 4 1.767 2.290 0.0926 0.5198 0.8791 900 0.3735 0.6317 0.3735 0.3280 0.4130 11.733 4 0.3735 0.6317 1050 0.1788 0.3023 0.1788 0.1170 0.2140 24.276 4 0.1788 0.3023 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94821143 0.844 -0.8175786 0.46062928 Bartlett's Test indicates equal varianc~(p = 0.21) 4.54906893 11.3448668 Hypothesis Test (1-tail, 0.05). NOEC _ _ _1.Q§C_~ ChV TU MSDu MS.Qp_ MSB MSE F-Prob df Dunnett's Test 750 >750 0.09263803 0.15668167 0.0040134 0.00327294 0.34279487 3, 12 Treatments vs D-Control Linear Inte!pOlation (200 Resamples) Point mg/L SD 95%CL(Ex2l Skew IC05* 315.80 185.01 86.12 1024.71 0.4865 IC10 686.81 132.81 139.11 839.84 -1.3149 IC15 767.63 52.55 575.49 832.99 -3.1043 IC20 797.95 33.93 638.23 857.16 -1.6553 IC25 828.27 28.80 688.64 881.73 -1.5389 IC40 914.44 14.68 866.48 950.31 -0.2463 IC50 959.98 11.87 922.86 990.27 -0.1105
- indicates IC estimate less than the lowest concentration Organisms obtdln(l.afrom Aquatox, Inc. atox02-l 0-09
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1.14 USEPA Control Limits (+/- 2 Standard Deviations) 1.12 1.10 1.08 1.06 -- --- 1.04 1.02 2.5 C' USEPA Warning and Control Limits (75 1h and 9dh Percentile CVs) u ~ 2.0 z
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r-1.4 1 Laboratory Warning and Control Limits (1 dh and 25 h Percentile CVs) 1.3 1.2 1.1 1.0 0.9 Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
- - . Central Tendency (mean IC25) - -**- WamingLimits(meaniC 25 +/-SA. 10 or SA. 75) 86 of 9S Control Limits (mean IC25 +/- SA25' SA. 90 , or 2 Standard Deviations) p ge -
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~ Ceriodaphnia dubia Chronic Reference Toxicant Control Chart ~ Environmental Testing Solutions, Inc. State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day IClS CT s Control Limits SA.1o Warning Limits s~ Control Limits S,.,,. Warning Limits sA... Control Limits cv (giL NaCI) (giL NaCI) CT-2S CT+2S CT- SA.IO CT + SA.lt CT - SA.lS CT + SA.lS CT- SA., CT + SA.,s cr- S...,0 cr +sA... 10-02-07 1.06 2 11~06-07 1.06 1.06 0.00 1.05 1.06 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.00 3 11-06-07 1.07 1.06 O.ot 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 4 12-04-07 1.07 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 5 01-08-08 1.09 1.07 0.01 1.04 110 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 6 Ol-08-08 1.09 1.07 0,01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.01 7 02-05-08 1.07 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.01 8 03-04-08 1.07 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.01 9 04-08-08 1.07 1.07 0.01 105 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.56 0.67 0.41 1.74 0.01 10 05-06-08 1.06 1.07 0.01 1.05 1.09 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 II 06-03-08 1.11 1.07 0.01 104 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.01 12 07-08-08 1.07 107 O.ot 105 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.01 13 08-05-08 1.09 1.08 O.Dl 1.05 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 O.Dl 14 09-09-08 1.10 1.08 0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 156 0.67 0.41 1.74 0.01 15 10-07-08 1.09 1.08 0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.49 0.59 1.56 0.67 0.41 1.75 0.01 16 11-04-08 1.07 1.08 0.01 105 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.75 0.01 17 IJ-04-08 1.10 1.08 0.02 105 1.11 0.09 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.56 0.67 0.41 1.75 0.01 18 12-02-08 1.06 1.08 0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.49 0.59 1.56 0.67 0.41 1.75 0.01 19 01-06-()9 1.05 108 0.02 105 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.01 20 02-03-09 105 1.08 0.02 1.04 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.02 Note: 7-d 1~ = 7-&y 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC2s). S = Standard deviation of the IC, values. La~ratory Control and Waraing Limits Laboratory control and warning limits were established using the standard deviation of the 1~ 5 values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. 111 s,\.10 =Standard deviation corresponding to the 10 percentile cv. (SAIO = 0.08) S~ = Standard deviation corresponding to the 25 111 percentile CV. (SA2s = 0.17) USEPA Control and Warning Umits SA.75 = Standard deviation corresponding to the 75fll percentile CV. (SA 75 = 0.45) SA,0 = Standard deviation corresponding to the 90111 percentile CV. (SA.90 =0.62) CV = Coefficient of variation of the I~ values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH. 02-03-09
** Precision of Endpoint Measurements Ceriodaphnia dubia Chronic Reference Toxicant Data ~ Environmental Testing Solutions~ Inc.
Test Control Control Mean number Test date Survival Reproduction CT cv CT MSD PMSD CT for Control Mean for Control (%) (offspring/female) Reproduction (%) Reproduction (%) forPMSD (%) (offspring/female) CV(%) 1 10-02-07 100 29.5 9.4 2.4 8.2 2 11-06-07 100 28.0 28.8 6.1 7.7 2.5 8.8 8.5 3 11-06-07 100 30.4 29.3 5.6 7.0 2.2 7.2 8.1 4 12-04-07 100 31.8 29.9 5.5 6.7 2.5 7.9 8.0 5 01-08-08 100 30.3 30.0 6.6 6.6 2.2 7.3 7.9 6 01-08-08 100 31.8 30.3 4.9 6.3 2.4 7.7 7.8 7 02-05-08 100 31.3 30.4 6.4 6.4 2.8 9.1 8.0 8 03-04-08 100 31.5 30.6 8.0 6.6 2.7 8.5 8.1 9 04-08-08 100 29.6 30.5 7.7 6.7 2.6 8.8 8.2 10 05-06-08 100 32.1 30.6 6.3 6.6 2.5 7.9 8.1 11 06-03-08 100 30.5 30.6 7.9 6.8 3.0 9.9 8.3 12 07-08-08 100 30.9 30.6 6.7 6.8 2.4 7.7 8.2 13 08-05-08 100 29.4 30.5 7.9 6.8 2.2 7.6 8.2 14 09-09-08 100 28.3 30.4 8.5 7.0 3.0 10.5 8.4 15 10-07-08 100 32.4 30.5 7.2 7.0 2.7 8.4 8.4 16 11-04-08 100 32.3 30.6 5.1 6.9 2.2 6.8 8.3 17 11-04-08 100 31.5 30.7 6.2 6.8 2.4 7.7 8.2 18 12-02-08 100 31.5 30.7 4.6 6.7 2.5 8.0 8.2 19 01-06-09 100 33.2 30.9 4.0 6.5 2.0 6.1 8.1 20 02-03-09 100 31.4 30.9 6.6 6.5 1.9 6.0 8.0 Note: CV = Coefficient of variation for control reproduction. Lower CV bound determined by USEPA (lOth percentile)= 8.9%. Upper CV bound determined by USEPA (90th percentile)= 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEP A (1Oth percentile) = 13%. Upper PMSD bound determined by USEPA (90th percentile)= 47%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200la, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. 02-03-09 Page 88 of98
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Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD) P ge 89 of 98 Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
Page I of6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia CdNaCLCR #: 85 Comments: Stock preparation: 600 1200 1400 9 15 18 21 1491 1488 1485 1482 1479 1500 1500 1500 1500 1500
< 24-hours old Selenastrum batch:
Daily renewal information: Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0 2 3 4 5 6 7 Control in ormation: Acceptance criteria S20%
~80%
% Mortali S:20%
Mean Offs ~ 15.0 offspring/female
%CV: <40.0%
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Page 2 of6 CdNaCLCR #: 8S Concentration:
% Mortality: I 07..
Mean Offspring/Female: 1 ,3\.'-\ 600 mg NCI/L a SUTVlVa. I andR epro duct'zon D at a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced n () 0 (") 0 0 0 0 0 0 Adult mortality L L L L L L L L L L 2 Young produced 0 0 0 0 0 0 0 0 0 a Adult mortality L L L L L L L L_ L \.... 3 Young produced 0 0 0 a 0 0 0 L 0 L cL 0 L Adult mortality L L L L L L 4 Young produced s '-\ l, lo '5 s '-\ '-\ s s Adult mortality L L L L L L L.. L L L
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6 Young produced ll.o \'S 1'-t Adult mortality \._ '-- \._ L \,_ '-- L \._ L. L ,, 7 Young produced Total young produced 3~ ..!..\ 31. 3S 2.~ 3~ o?> 3'2... .3\ 30 Final Adult Mortality L L '- L L L. L l l L. Note: Adult mortality (L- live, D- dead), SB- spht brood (smgle brood spht between two days), CO- carry over (offsprmg carried over with adult during transfer). Concentration:
% Mortality: Q7..
Mean Offspring/Female: 32..0
% Reduction from Control: ---V\7o !"*,.
91 of98
Page 3 of6 Species: Ceriodaphnia dubia CdNaCLCR #: &S
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Final Adult Mortality \.._ L L \.... L L.. L. \..... L. '- Note: Adult mortaltty (L- hve, D =dead), SB = spltt brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer). 1000 mg NCI/L a S urvzva
. l an dR epro duct"zon D at a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced .0 0 0 () 0 0
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7 Young produced Total young produced 3C ~\ "'L$ "l..C\ l.'\ 2.<o 3\ 2'\ '2..<1\ Zi L. L -c.:* '- '- Final Adult Mortality L '- L. L \.... Note: Adult mortality (L"' hve, D "'dead), SB =split brood (single brood split between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration:
% Mortalitv: 07.
Mean Offsoring/Female: "2&*,
%Reduction from Control: g .(,7, 92 of98
Page 4 of6 Species: Ceriodaphnia dubia CdNaCLCR #: &S 1200 mg Na CIIL S urvzva. I an dR eproduc r*zon D ata Replicate number
, . **Day 1 2 3 4 5 6 7 8 9 10 o-*
1 Young produced 0 0 0 0 D 0 0 0 0 D Adult mortality L L L '- '- L L '- \-. L 2 Young produced 0 0 a 0 a 0 0 0 0 0 Adult mortality L L l.... L L L
'- L \..._ L 3 Young produced D 0 0 0 0 0 0 C) 0 0 Adult mortality \...... ... ~ Ll- L L. L.. '-- L '- L 4 Young produced ~ '-\ ~ s ~ 3 t..t Ll 4 4 Adult mortality L L L \._ '- L '- \.._ \...._
5 Young produced Q ~ s ~ I. \c. 0 s s L... Adult mortality L- '- L '- L L '-- '- L. '-- 6 Young produced 0 0 0 0 0 0 \.o 0 0 5 Adult mortality '- L. \..... L. L L \...... '-- L '- d 7 Young produced Total young produced
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% Mortality: o1.
Mean Offspring/Female: &A I I 1400 mg NCIIL a S urvzva
% Reduction from Control:
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2 Young produced 0 L 0 0 0 L-0 0 () () a 0 l_ Adult mortality L '-- L L L L L 3 Young produced 0 0 0 D 0 0 0 0 0 0 Adult mortality L '- L L L L. '- L L L 4 Young produced 0 1.. '1.. \ 1... D ~ 0 0 -z..... Adult mortality L '- L L L L L '- L L 3 a ~ D 4 _Q
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Adult mortality L L L. L- L '- L. L- L L 6 Young produced D D- 0 D 0 0 0 0 6 Adult mortality L \.,_ L L L \...... L L. \... L. \} 7 Young produced Total young produced .:!, 2.. L. .a. "2.. -3 3 4 I l.. Final Adult Mortality L L '-- L. '- \...... L. '- '-- L. Note: Adult mortahty (L = hve, D =dead), SB =split brood (smgle brood spbt between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: IJ % Mortality: Q1. It Mean Offspring/female: '2.*'5
%Reduction from Control: 'Jl,.() 7~
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1..... ..)J Concentration Replicate number Survival Average reproduction Coefficient of Percent reductiou from ("/o) (offspring/female) variatioa (%) ooatrol (%) (mg!L NaCI) I 2 3 4 5 6 7 8 9 10 Control 31 31 33 30 28 35 30 34 31 31 100 31.4 6.6 Not applicable 600 33 31 32 35 29 34 33 32 31 30 100 32.0 5.7 -1.9 800 30 28 33 34 31 32 32 31 30 33 100 31.4 5.7 0.0 1000 30 31 25 29 29 26 31 29 29 28 100 28.7 6.8 8.6 1200 3 7 8 8 10 9 10 9 9 11 100 8.4 26.4 73.2 1400 3 2 2 3 2 3 3 4 1 2 100 2.5 34.0 92.0 Dunnett's MSD value: 1.876 MSD= Minimum Significant Difference PMSD: 6.0 PMSD= Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (lOth percentile). = 13%. Upper PMSD bound determined by USEPA (90th percentile)= 47%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data [Tom EPA's WET Interlaboratory Variability Study (USEPA. 200la; USEPA. 2001b). USEPA. 2001 a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01 -004 and EPA-821-B-0 1-005. US Environmental Protection Agency, Cincinnati, OH.
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CeriodaEhnia Swvival and R!:Eroduction Test-R!:Eroduction Start Date: 2/3/2009 Test ID: CdNaCICR SampleiD: REF-Ref Toxicant End Date: 2/9/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mg/L 1 2 3 4 5 6 7 8 9 10 D-Control 31.000 31.000 33.000 30.000 28.000 35.000 30.000 34.000 31.000 31.000 600 33.000 31.000 32.000 35.000 29.000 34.000 33.000 32.000 31.000 30.000 800 30.000 28.000 33.000 34.000 31.000 32.000 32.000 31.000 30.000 33.000 1000 30.000 31.000 25.000 29.000 29.000 26.000 31.000 29.000 29.000 28.000 1200 3.000 7.000 8.000 8.000 10.000 9.000 10.000 9.000 9.000 11.000 1400 3.000 2.000 2.000 3.000 2.000 3.000 3.000 4.000 1.000 2.000 Transfonn: Untransfonned !-Tailed Isotonic Cono-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 31.400 1.0000 31.400 28.000 35.000 6.578 10 31.700 1.0000 600 32.000 1.0191 32.000 29.000 35.000 5.705 10 -0.731 2.287 1.876 31.700 1.0000 800 31.400 1.0000 31.400 28.000 34:.000 5.657 10 0.000 2.287 1.876 31.400 0.9905
*1000 28.700 0.9140 28.700 25.000 31.000 6.782 10 3.290 2.287 1.876 28.700 0.9054
*1200 8.400 0.2675 8.400 3.000 11.000 26.442 10 28.029 2.287 1.876 8.400 0.2650
*1400 2.500 0.0796 2.500 1.000 4.000 33.993 10 35.219 2.287 1.876 2.500 0.0789 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates nonnal distribution (p > 0.01) 0.95103425 1.035 -0.5620922 0.76156552 Bartlett's Test indicates equal variances (p = 0.18) 7.56523657 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 800 1000 894.427191 1.87636754 0.05975693 1771.72 3.36666667 1.7E-44 5, 54 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point m!i!: SD 95%CL Skew IC05 895.185185 39.6216408 798.428309 967.186364 -0.4820 IC10 1001.67488 24.6399956 926.84517 1012.24105 -1.3664 IC15 1017.29064 6.93088301 1003.19782 1027.02713 -1.7330 IC20 1032.9064 5.81256688 1019.78841 1041.72271 -0.5668 IC25 1048.52217 5.49826611 1036.37899 1057.13228 -0.5823 IC40 1095.36946 4.95417378 1084.62237 1102.73588 -0.5583 IC50 1126.60099 4.99006093 1115.31017 1133.67544 -0.4842 02-03-09
Page 5 of6 Species: Ceriodaphnia dubia CdNaCLCR #: &S CONTROL 600 mg NaCI!L 800 mg NaCI!L 1000 mg NaCI/L 1200 mg NaCI/L 1400 mg NaCI!L I ~ST_O_C_K------~~~~--1---~ tage 97 of98 ~\'
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PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No 2040-0004 (SUBR 01) Add res§_ _E.Q;_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ F- FINAL ---~TEROFFICESB*~---------- - _ _ _.§.ODD_r:_-_Qt.ISY _ _IN 3738L_ _ _ _ _ _ _ _ TN0026450 I! 103 G LOW VOL. WASTE TREATMENT POND Facility_ JYA -_§f:QUOY/lli.NUCLEAR PLANT_ _ _ _ _ _ Locatio.!!_ J::!AMIL TOl:!_COUNTY __________ _ EFFLUENT ATIN: Stephapie A Howard From 1 Y~R I ~2 *r~A~v T. *~: f ;~~;~ ~~ ~A; I *** NO DISCHARGE CJ ... NOTE: Read instructions before completinq lh1s form. PARAMETER QUALITY OR CONCENTRATION I NO. !FREQUENCY I SAMPLE EX I OF I TYPE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS H ******** 7.3 ******** 8.5 0 GRAB 12 00400 0 0 GRAB EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED GRAB 00530 0 0 GRAB EFFLUENT GROSS VALUE OIL AND GREASE GRAB 00556 0 0 DUITORTHRU PLANT 1 0 0 FFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance w1th a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the mformation submitted. Based on my inquiry of the person or persons who manage the system, Or those persons dtrectly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE' f - - - - - - - - - - - - - - - - - - - - - - 1 i n f o r m a t i o n , includmg the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEARPLANT ___ _ DISCHARGE MONITORING REPORT (OMRJ OMS No. 2040-0004 (SUBR 01) Add res.§_ _E.~ BOX 20Q.Q.. _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 107 G F- FINAL - _ _ _§_ODDY- DAISY_ _JN 3738L _ _ _ _ _ _ _ METAL CLEANING WASTE POND Facility TVA- SEQUOYAH NUCLEAR PLANT Location HAMIL TON COUNTY ========== YEAR DAY EFFLUENT NO DISCHARGE [~ *** ATTN: Stephanie A. Howard From I 09 I -- I -- I 28 NOTE Read instructions before completinq th1s form PARAMETER QUALITY OR CONCENTRATION i NO. II FREQUENCY! SAMPLE EX OF i TYPE ANALYSIS ! PH 0 0 GRAB LIDS, TOTAL SUSPENDED 0 0 MG/L DAILY I COMPOS AND GREASE 19 0 0 DAILY GRAB TOTAL (AS P) 1 0 0 EFFLUENT GROSS VALUE COPPER, TOTAL (AS CU) 01042 1 0 0 MG/L DAILY FFLUENT GROSS VALUE RON, TOTAL (AS FE) 19 0 0
, IN CONDUIT OR THRU ITDCATA1ENT PLANT 1 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law lhat.this document and all attachments w.ere prepared under my ~ 1'\. ~ I TELEPHONE DATE I' d~rection or supervis1on in accordance with a system des1gned to assure that qualified personnel ~ j Timothy P Cleary properly gather and evaluate the mformation submitted. Based on my 1nquiry of the person or
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Sequoyah Site Vice President accurate, and complete I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing VIOlations. SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 1 YEAR MO
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PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) MAJOR Form Approved Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Add res.§_ _E.!1_;BOX..1.QOO _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 110 G F- FINAL ---~TEROFFICESB*~---------- ---~DDY-DAISY_~N3738L ______ _ RECYCLED COOLING WATER Facility_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!L .J.:!AMILTO!::LCOUNTY __________ _ EFFLUENT YEAR DAY 28 NO DISCHARGE I XX I ... ATTN: Stephanie A. Howard From I 09 I -- I -
- I NOTE: Read instructions before completinq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. !FREQUENCY SAMPLEl EX I OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 1 ANALYSIS :
TEMPERATURE, WATER DEG. ******** ******** ******** 04 CENTIGRADE 00010 z 0 0 DAILY GRAB-4 INSTREAM MONITORING PH 00400 1 0 0 WEEKLY I GRAB EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 0 0 DAILY I COMPOS 1 I EFFLUENT GROSS VALUE OIL AND GREASE 00556 0 0 DAlEY GRAB EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT
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PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) AddresL _..E.~BOX..1QOO _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 110 T F- FINAL ---~TEROFFICESB-~---------- _ _ _ 20DDY-DAISY_~N3738L _ _ _ _ _ _ _ RECYCLED COOLING WATER Facilitv_ JYA -_§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!!_ _i:jAMIL TOJi.COUNTY __________ _ EFFLUENT Fr om YEAR 09 DAY 28 NO DISCHARGE IXXl *** ATTN: Stephanie A. Howard NOTE: Read instructions before completin~ this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. !FREQUENCY SAMPLE EX OF TYPE t AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 1 ANALYSIS IC25 STATRE 7DAY CHR ******** ******** ******** ******** CERIODAPHNIA 23 1 TRP3B 1 0 0 SEMI EFFLUENT GROSS VALUE ANNUAL IC25 STATRE 7DAY CHR 23 PIMEPHALES TRP6C 1 0 0 SEMI COMPOS EFFLUENT GROSS VALUE ANNUAL j l I RI
~(1-~y~ I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE 'I direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inqUiry of the person or I
persons who manage the system, or those persons directly responsible for gathering the ! Principal Environmental Engineer Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 1 og 03 10 1 accurate, and complete. I am aware that there are sigmficant penalties for submitting false Information. including the possibility of fine and imprisonment for knowing violations SIGNATURE OF PRINCIPAL EXECUTIVE I l J TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA I NUMBER IYEAR I MO DAY I CODE _j____J COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L .£.-~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 116 G F- FINAL ---~DDY-DAISY_~N3738L _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER I BACKWASH Facility_ JYA -_§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!}_ _!:!AMIL TOJ:LCOUNTY._ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT YEAR DAY ATIN: Stephanie A. Howard From 09 To 28 NO DISCHARGE li *** NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. !FREQUENCY I SAMPLE EX OF I TYPE ANALYSIS AVERAGE I MAXIMUM I UNITS I MINIMUM I I J I UI::C"'"* FLOATING (SEVERITY) ******** I ******** I I ******** I ******** I 0 I 9A 0 1 I 28 VISUAL 1 0 0 PASS=O SEE VISUAL NT GROSS VALUE FAIL=1 PERMIT
.. I 0 II 1 I 28 VISUAL 0 0 I SEE VISUAL EFFLUENT GROSS VALUE PERMIT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE I direction or supervision in accordance w1th a system designed to assure that qualified personnel 1------- -----,--~--
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. . . !information, the information submitted is , to the best of my knowledge and belief, true, 03 10 Sequoyah Srte Vice President accurate, and complete 1 am aware that there are significant penalt1es for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE 1--------------------------l*information, includ1ng the possibility of fine and imprisonment for knowing violatiOns OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Operations performs visual inspections for floating debris and oil and grease during all backwashes'. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FacilitY Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ Form Approved. MAJOR Name_~A-SEQUOYA~UCLEARP~NT ___ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres.2._ .J:..~BOX~OO ___________ _ TN0026450 117 G F- FINAL ---~TEROFFICESB-~---------- ---~DDY-DAISY_~N3738L ______ _ BACKWASH Facility_ .JYA -~QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l_ _jjAMIL TOJ'i.COUNTY __________ _ EFFLUENT YEAR DAY NO DISCHARGE [J ~** ATTN: Stephanie A. Howard From I 09 I -- I - - I 28 NOTE: Read instructions before completinq th1s form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. IFREQUENCY/ SAMPLE EX OF i TYPE AVERAGE MAXIMUM UNITS MINIMUM I AVERAGE I MAXIMUM I UNITS DEBRIS, FLOATING (SEVERITY) ******** ******** ******** ******** 0 9A 01345 1 0 0 VISUAL EFFLUENT GROSS VALUE OIL AND GREASE. VISUAL VISUAL 84066 0 0 VISUAL EFFLUENT GROSS VALUE I '
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----,---- i 1 I J~Q~rar{ J NAME!TITLE PRINCIPAL EXECUTIVE OFFICER 1Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel I I Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or II i persons who manage the system, or those persons directly responsible for gathering the i
Principal Environmental Engineer 423 843-6700 09 i o3 I 10 \ Sequoyah Site Vice President Information, the information submitted is . to the best of my knowledge and beli.ef, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing Violations. SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREAl I NUMBER l l YEAR I MO l I DAY J l! TYPED OR PRINTED CODE I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMS No. 2040-0004 (SUBR 01) Addres_L _E.~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 118 G F- FINAL - _ _ ...§.ODDY- DAISY_ _IN 37381._ _ _ _ _ _ _ _ WASTEWATER & STORM WATER Facility TVA- SEQUOYAH NUCLEAR PLANT Lo~tion HAMILTONCOUNn========== EFFLUENT YEAR NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard From I 09 I -- I - - I PARAMETER QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM
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OXYGEN, DISSOLVED (DO) ******** ******** ******** ******** 19 i, 00300 0 0 ******** MG/L TWICE/ EFFLUENT GROSS VALUE WEEK SOLIDS, TOTAL SUSPENDED ******** 19 i 00530 1 0 0 MG/L T\tiJICE/ I> GRAB EFFLUENT GROSS VALUE WEEK SOLIDS, SETTLEABLE 25 00545 0 0 MLIL ONCE/ GRAB EFFLUENT GROSS VALUE MONTH FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 0 ESTIMA EFFLUENT GROSS VALUE I I I
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i I I L NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my Timothy P. Cleary direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Oc TELEPHONE DATE I Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 09 03 10 Sequoyah Site Vice President accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA MO NUMBER YEAR DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
ENVIRONMENTAL SUBMITTAL FEBRUARY DMR PLEASE SIGN AND RETURN TO Ann Hurt EXT. 6714 DUE IN MAIL TO TDEC BY March 12, 2009
S58 090413 800- NPDES CORRESPONDENCE April 13, 2009 State ofT ennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR MARCH 2009 Enclosed is the March 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sin:;:~ ~e~ Timothy P. Cleary V Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN B. A. Wetzel, OPS 4A-SQN T. P. Cleary, OPS 4A-SQN G. R. Signer, WT 6A-K D. E. Pittman, BR 4T-C EDMS, WT CA-K (Enclosure) DMR0903.doc
REVIEW /CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- MARCH DMR ORGANIZATION: Chemistry and Environmental DOCUMENT PREPARED BY: Ann Hurt DATE: 04/09/2009 EDMS TRACKING NO.: S58 0904 800- NPDES Correspondence CONCURRENCES Name R c Signature - Comment Date v N Ann Hurt X Stephanie A Howard X Alan K Barringer X Debra J Bodine X Beth A. Wetzel X Chris R Church X Timothy P Cleary X (for signature) ~ I >Af~di. INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
Tennessee Valley Authority Post Office Box 2000 Soddy Daisy, Tennessee 37384-2000 Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant April 13, 2009 State ofT ennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR MARCH 2009 Enclosed is the March 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely, ~1~ Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 pnnted on recycled paper
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~~~~-~~~---
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,S~~(}_li_alk/oAd l_!"_AMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s ~ocument and all attachments were prepared under my TELEPHONE I DATE direction or supervision in accordance wtth a system des1gned to assure that qual1f1ed personnel Timothy P Cleary properly gather and evaluate the 1nformat1on submitted Based on my inqu~ry of the person or r
I persons who manage the system, or those persons directly responsible for gathering the informat1on, the informat1on subm1tted 1s , to the best of my knowledge and belief, true, Principal Environmental Engineer 423 843-6700 09 I 04 09 c I Sequoyah Site Vice President TYPED OR PRINTED accurate, and complete I am aware that there are Significant penalties for subm1tt1ng false information, including the possibility of ftne and tmpnsonment for knowtng violations COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: 1. CCW data 2. veliger monitoring data SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE I I NUMBER Y-EARr-M'O - DAY EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2
DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 03/11/2009@ 1232 0.15mg/l 03/12/2009@ 1721 KLM TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 03/11/2009@ 1234 <0.11 mg/1 03/12/2009 @ 1732 KLM TN EPA 8015
Mean# of NOTES:% Mean# of Water Temp. Water Temp. LOCATION SUB Sample Date % Settlers ('C) Sample Date Asiatic Gravid Asiatic COLLECTED BY ZM/m3 ('C) LOCATION Clams/m3 Clam 11/21/2008 0 0 12 11/16/2008 230 12 In plant RCW Dick Adcock 11/24/2008 31 0 11 11/24/2008 138 11 In plant RCW Dick Adcock 12/4/2008 0 0 10 12/04/2008 15 10 In plant RCW Dick Adcock 12/812008 0 0 9 12/08/2008 0 9 In plant RCW Dick Adcock 12/1512008 0 o* 9 12/15/2008 0 9 In plant RCW Dick Adcock 12/2212008 0 0 9 12/22/2008 0 9 In plant RCW Dick Adcock 12129/2008 0 0 9 1212912008 0 9 In plant RCW Dick Adcock 01/09/2009 1"1 v 0 Q u 01/09/2009 0 8 In plant RCW Dick Adcock 0111612009 0 0 7 0111612009 0 7 In plant RCW Adcock I Bryant 01122/2009 0 0 7 0112212009 0 7 In plant RCW Adcock I Bryant 01/30/2009 0 0 9 01/3012009 0 9 In plant RCW Adcock I Bryant 0210612009 0 0 6 0210612009 0 6 In plant RCW Adcock I Bryant 0211312009 0 0 11 0211312009 0 11 In plant RCW Love 0212012009 0 0 9 0212012009 0 9 lnplant RCW Bryant 0212612009 0 0 9 0212612009 0 9 In plant RCW Bryant 0310612009 0 0 8 0310612009 0 8 In plant RCW Dick Adcock 0311312009 0 0 10 0311312009 0 10 In plant RCW Dick Adcock 0312712009 0 0 14 0312712009 0 14 In plant RCW Wayne Wright
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Add res.§_ _E ~BOJ<.19QQ_ _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 G F- FINAL ---~TEROFFICESB-2A-SQ~-------- - _ _ _§ODD_1_- DAISY_ _:fl::!.]738i_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facility_ _IYA -.§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!]_ ___I::!AMIL TO.!i_COUNTL _ _ _ _ _ _ _ _ _ _ M.illllTORING PERIOD EFFLUENT From YEAR DAY
*** NO DISCHARGE D ...
ATTN: Stephanie A. Howard 09 To 31 NOTE: Read instructions before completinq this form. PARAMETER auA-:N=T=ITY=-=--o::cR=-=-L-=o-=A-=o:::IN:::G:--~---,--------a::cu,.,.A"'L,...,I=TY--::--::o-=R-:c::-co=-=N-:-c=-=E=-:N:::T==R:c-:ATION I No. [FREouENCY SAMPLEl EX I OF ANALYSIS TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS CHLORINE, TOTAL RESIDUAL ******** ******** ******** 0.018 0.036 0 18 I 31 GRAB 19 150060 1 0 0 PERMIT ******** "'>.-:()J()';~!'l I REQUIREMENT :*, . '>:<.: EFFLUENT GROSS VALUE * .MO AYG 1 ,,~.., * ......-,"". 1 1* 1 .** _ 1 :_:. --~.:_ 1 TEMPERATURE- C, RATE OF ******** 2 62 ******** 'CHANGE 82234 0 0 EFFLUENT GROSS VALUE PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMrf. :- {REOUIREMEN'r-:'
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SAMPLE MEASUREMENT PERMIT*** REqf:)JRE:M~NT SAMPLE MEASUREMENT PERMIT REQUIREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ,1 Cert1fy under penalty of law that th1s document and all attachments were prepared under my
~9 1 TELEPHONE
~ d"ection or superviSion in accordance with a system des*gned to assure that qualified personnel Timothy P. Cleary *properly gather and evaluate the information submitted. Based on my inqu1ry of the person or 1 I persons who manage the system, or those persons d"ectly responsible for gathemg the Principal Environmental Engineer
. . . information, the information submitted is , to the best of my knowledge and bel1ef, true, 423 843-6700 09 04 09 Sequoyah S1te V1ce PreSident accurate, and complete. 1 am aware that there are s*gn1f1cant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE 1nformat1on, including the possibility of tine and Imprisonment for know1ng violations OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED AREA CODE NUMBER YEAR MO I DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
The following injections occured: 1. Biodetergent 73551 (max. calc. cone. was 0.027mg/L--Iimit 2.0mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _E.fLBOX..1.QQQ_ _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 Q F- FINAL
---~TEROFFICESB-2A-SQ~--------
_ _ _ 20D~DAISY_~N3738L _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facility_ JYA -_g:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatioll_ _!:jAMIL TOJ:i.COUNTL _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT ATIN: Stephanie A. Howard From Y~R I ~~ I ~A1Y I To ~t ~~ I ~A1Y NO DISCHARGE D *** NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. !FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS BORON, TOTAL ******** ******** <0.85 0 2/90 19 I 1 o1 022 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIBE'MENT. SAMPLE MEASUREMENT
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REOl:JIREME~}IY SAMPLE MEASUREMENT PERMIT REQUIR8.M,ENT Att~teuu.£ 0, cikwcv<.d FMEITITLE PRINCIPAL EXECUTIVE OFFICER I Cerlify under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE d1rection or supervision 1n accordance w1th a system designed to assure that qualified personnel
' Timothy P. Cleary properly gather and evaluate the mformation subm1tted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer
. . . information, the information submitted IS , to the best of my knowledge and belief, true, 423 843-6700 09 04 09 Sequoyah Site V1ce Pres I~ accurate, and complete. 1am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED tnformatlon, tnclud1ng the posstbtllty of fine and 1mpnsonment for know1ng vtolations.
OFFICER OR AUTHORIZED AGENT AREA CODE l I NUMBER YEAR _ _ L_ MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Boron was sampled on 01/07/2009@ 1005. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres.L_ .J: ~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _
---~TEROFFICESB-2A-SQ~-------- TN0026450 101 T F- FINAL
- _ _ __§_ODDY- DAISY _ _JN 3738~ _ _ _ _ _ _ _ BIOMONITORING FOR OUTFALL 101 Facility_ JYA -~QUOYAH NUCLEAR PLANT:_ _ _ _ _ _ Locatio.!l._ .J:!AMIL TO.!!_COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard From DAY 31
*** NO DISCHARGE D ...
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.J~phCul-~Q.cf.lowcL~ d NAMErTITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direction or supervision m accordance w1th a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer
~
Information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 09 04 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, 1ncluding the poss1b1lity of fine and 1mpnsonment for know1ng VIOlations. OFFICER OR AUTHORIZED AGENT AREA CODE l NUMBER YEAR MO COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in March 2009. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Addres.§_J:.~BOX2000 - - - - - - - - - - - - TN0026450 103 G F- FINAL ---~TEROFFICESB-2A-SQ~-------- _ _ _ ~ODD_.:I_- DAISY_ __I!::!-'!7381._ _ _ _ _ _ _ _ LOW VOL. WASTE TREATMENT POND Facility_ JYA -_§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l._ _HAMIL TO.!:!_COUNTy_ _ _ _ _ _ _ _ _ _ _ EFFLUENT DAY
*** NO DISCHARGE D ...
ATTN: Stephanie A. Howard 31 PARAMETER '"j<i QUANTITY OR LOADING QUALITY OR CONCENTRATiON___ NOTE: Read instructions before completinq this form. FREQUENCY OF I SAMPLE TYPE AVERAGE MAXIMUM MINIMUM AVERAGE MAXIMUM ANALYSIS 1 PH ******** ******** 6.9 ******** 8.6 0 15 I 31 GRAB 12
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Jtcl,phecr~..t.LQ\4!-{__'Ct. ~d NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE I direction or supervision in accordance with a system des1gned to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inqUiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer I I information, the information submitted 1s , to the best of my knowledge and belief, true, 423 843-6700 09 04 I 09 Sequoyah Site Vice President accurate, and complete I am aware that there are S1gnif1cant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE I I
~ TYPED OR PRINTED information, including the possibility of f1ne and impnsonment for know1ng VIOlations OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER IYEAR MO l DAY 'COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres,L _f ~BOJ<..1900 ___________ _ TN0026450 107 G F- FINAL ---~TEROFFICESB-2A-SQ~-------- - _ _ __§_ODD_Y._- DAISY_ _IN 37381_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER METAL CLEANING WASTE POND Facility_ .J.YA -_lli:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ LocatiOIL. _jjAMIL TOl!_COUNTY._ _ _ _ _ _ _ _ _ _ _ EFFLUENT YEAR DAY
*** NO DISCHARGE I XX I ***
ATTN: Stephanie A. Howard From 09 31 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. EX IFREQUENCY SAMPLE I OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS
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[EFFLUENT GROSS VALUE * *MQ A.V,G.*.* .. . DAILYMX . L _ __ _ ...L-o..e~~~=~ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this document an.d all attachments were P. repared under my q Lj.-, ~ j ol J! . d. TELEPHONE DATE J I direCtiOn or superv1s1on in accordance w1th a system des1gned to assure that qual1f1ed personnel LJ!4)t'lli.J~ \S1:i0l'~\('L-{_ Timothy P. Cleary properly gather and evaluate the 1nformat1on subm1tted. Based on my 1nqUify of the person or ! personswho manage the system. or those persons directly responsible for gathenng the Principal Environmental Engineer
. . . 1nformat1on, the information submitted IS , to the best of my knowledge and bel1ef, true, 1 423 843-6700 04 09 1
09 Sequoyah S1te V1ce President accurate. and complete 1am aware that there are significant penalt1es for submtttlng false SIGNATURE OF PRINCIPAL EXECUTiVE! _ 'I i
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*-------------1'nformatlon. 1nclud1ng the poss1b1l1ty of fine and 1mpnsonment for know1ng VIOlations TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT-I CODE NUMBER YtoAKI MO i DAY I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ Form Approved. MAJOR Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Add res.§_ .J:.~BOX.1QOO _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 110 G F- FINAL ---~TEROFFICESB-2A-SQ~-------- - _ _ _§ODDY- DAISY_ _IN 3738+/-_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facility_ ~A -.§l:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.o_ __!:!AMIL TO.!'!_COUNTY __________ _ EFFLUENT YEAR DAY NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard From 09 31 NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRA"IIUN -*--. NO. [FREQUENCY EX I OF AI\JAIYSIS PH
---~~~~~J~:~~~~,_,-1-~;m;:~~-~~ l .... _..... -....
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'EFFLUENT GROSS VALUE DAILYMX CHLORINE, TOTAL RESIDUAL SAMPLE ********
MEASUREMENT 50060 0 0 PERMIT******* -;;,,~tfHp.1~;;,;; MG/L GRAB-4 REQUIREMENT EFFLUENT GROSS VALUE DAILYMX. L *.x. I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER _1t~cu~..u.O,z.~jvl0ev--d I Cert1fy under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direction or superviSIOn in accordance w1th a system des1gned to assure that qualified personnel i Timothy P Cleary properly gather and evaluate the information submitted Based on my 1nqu1ry of the person or I Sequoyah Site Vice President persons who manage the system, or those persons d1rectly respons1ble for gathenng the 1nformat1on, the 1nformatton submitted is , to the best of my knowledge and belief, true. accurate, and complete I am aware that there are Slgnif!cant penalties for submitting false Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE 423 843-6700 09 04 lI 09 I c TYPED OR PRINTED 1nformat1on, 1ncludmg the possibil1ty of fine and Imprisonment for knowtng VIOlations. OFFICER OR AUTHORIZED AGENT AREA CODE I I NUMBER YEAR MO l DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _E ~B0)(_1900 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 F- FINAL
---~TEROFFICESB-2A-SQ~-------- - _ _ _§ODD_::f._- DAISY _ _IN 37384.._ _ _ _ _ _ _ _ PERMIT NUMBER RECYCLED COOLING WATER Facility_ JYA -~QUOYAH NUCLEAR PLANT_ _ _ _ _ _
Locatio~ _!:!AMIL TOJ:i_COUNTJ'_ _ _ _ _ _ _ _ _ _ _ EFFLUENT YEAR NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard From! 09 I -- ~ NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREOUENCYI SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR ******** ******** ******** ******** CERIODAPHNIA TRP3B 1 0 0 SEMI c
!cOMPOS IEFFLUENT GROSS VALUE IC25STATRE7=D~A~Y~C~H~R~-------T--~S~A~M~PL~E~--T----~~---r--~------~------~~**~~~~*~~~~~**~*~u**~*~~~~--~-+-=~~~~~------~~~~---+~--~
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L ___ I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER c'PERMIT ..... . REOtii~§:MENt I J{Q.p~<iJ'l_ti_ Qdkru_xu_d I Cert1fy under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervtsion tn accordance w1th a system designed to assure that qualified personnel Timothy P Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or I
! persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer I
I Sequoyah Site Vice President informat1on, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE 423 J 843-6700 091 04 09 i TYPED OR PRINTED Information, 1nclud1ng the poss1bil1ty of fme and Imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT 'AREA CODE I NUMBER YEARJ MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMIITEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) MAJOR Form Approved. Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) AddresL _£.~B0)(_19QQ_ _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 116 G F- FINAL ---~TEROFFICESB-2A-SQ~-------- I
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BACKWASH Facility_ JYA -..§EQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l._ _!:!AMIL TOJ:i.COUNTY __________ _ EFFLUENT ATTN: Stephanie A Howard From I 09 YEAR I n I -
- I DAY 31 NO DISCHARGE CJ ***
NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. [FREQUENCY[ SAMPLE EX OF [ TYPE ANALYSIS AVERAGE MAXIMUM MINIMUM AVERAGE MAXIMUM UNITS DEBRIS, FLOATING (SEVERITY) ******** ******** ******** ******** 0 0 1 I 31 VISUAL 9A 01345 1 0 0 PASS=O [EFFLUENT GROSS VALUE FAIL=1 ol IVI""L. I lOlL AND GREASE VISUAL ******** VISUAL
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EFFLUENT GROSS VALUE 1;\~~~~~~U~fur SAMPLE MEASUREMENT PERMIT REQUIREMENT i NAME/TITLE PRINCIPAL EXECUTIVE OFFICER
..J.tQ.pfw0~ 0, .ilo1.uc(.."- d it Cert1fy under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE I !?lrect1on or superviSIOn 1n accordance w1th a system des1gned to assure that qual1f1ed personnel I Timothy P CI"'Y
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persons who manage the system or those persons d1rectly responsible for gathenng o1 Information the 1nformat1on submitted 1s to the best of my knowledge and bel1ef, true, the m
"""" Principal Environmental Engineer 423 843-6700 09 04 09 Sequoyah Stte Vtce Prestdent accurate and complete 1 am aware that there are s1gmf1cant penalt1es for submtt!ing false SIGNATURE OF PRINCIPAL EXECUTIVE I 1nformat1on 1ncJudJng the possibility of fme and 1mpnsonment for know1ng VIOlations OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR I MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Add res§_ .J:..~BOX.1.QOO _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A-SQ~-------- C=rNoo264so [ 117 G F- FINAL - _ _ _§_ODD_1_- DAISY _ _It:!.]7381_ _ _ _ _ _ _ _ I PERMIT NUMBER DISCHARGE NUMBER BACKWASH Facility_ _I!A -.§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!]_ ..J:::!AMIL TO~COUNTY __________ _ MONITORINr, PERIOD EFFLUENT I MO I DAy I I YEAR I MO I DAy From YEAR 09 I 03 I 01 I Tor 09 1 03 I 31 NO DISCHARGE CJ *** ATTN: Stephanie A. Howard NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. IFREQUENCYI SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM MINIMUM AVERAGE MAXIMUM UNITS DEBRIS, FLOATING (SEVERITY) ******** ******** ******** ******** 0 0 1 /31 VISUAL 9A 01345 1 0 0 ********', ********' EFFLUENT GROSS VALUE OIL AND GREASE VISUAL ******** 0 1 ~~~~UEN~ GR~SS VALUE 0 I
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- m I MEASUREMENT I NAMEfTITLE PRINCIPAL EXECUTIVE-OFFICER-TI Cert1fy under penalty of law that this document and all attachments were prepared under my 1.1-:- J .* (\ II ' . *' TELEPHONE DATE I Timothy P. Cleary
-jdlrect1on or superviSion 1n accordance w1th a system destgned to assure that qualified personnel
[properly gather and evaluate the information submitted. Based on my inqwy of the person or yt\.L(J"'\0.-tt..U. LA ~ll.A.JUA..d . persons who manage the system, or those persons directly respons1ble for gathenng the I I . . , information, the information submitted !S , to the best o~ my knowledge and belief, .true, Principal Environmental Engineer 423 843-6700 09 04 ::9Y I 1 Sequoyah S1te V1ce President accurate, and complete 1am aware that there are s1gnlf1cant penalties for submitt1ng false SIGNATURE OF PRINCIPAL EXECUTIVE I ! I Iinformation, 1nclud1ng the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREAl NUMBER IYEAR MO DAY TYPED OR PRINTED , CODE , COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location 1f Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved Name_~A~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Add res.§_ ___E.~BOlV.QOO _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A-SQ~-------- TN0026450 118 G F- FINAL
---~DDY-DAISY_~N3738L ______ _ PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER Facility_ JYA -.§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _
Location_ J::!AMILTO_!::!_COUNTY __________ _ MQNITORING PEflli2D EFFLUENT YEAR I YEAR I MO DAY NO DISCHARGE I XX I *** ATIN: Stephanie A. Howard From I 09 To 09 03 31 NOTE: Read instructions before completinQ this form. I PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.-rREQUENCY SAMPLE EX OF TYPE 1, i ANALYSIS 1 ' AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM IOXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT
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[_NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direction or supervision 1n accordance with a system designed to assure that qua!1fied personnel I II Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inqUiry of the person or II persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer b_~h Site v;oo p,,;dool Information, the 1nformat1on subm1tted IS , to the best of my knowledge and belief, true, 423 843-6700 I 09 04 09 accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I I PED OR PRINTED tnformat1on, 1nc!uding the poss1b1hty of fine and imprisonment for know1ng VIOlations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBERtvEAR l MO DAY l COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
S58 090514 800- NPDES CORRESPONDENCE May 14, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR APRIL 2009 Enclosed is the April 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at {423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely,.
---- -~--. II (1. JJ"Q'
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"'-'<V-~JJ. .\1,. '~ !jJ_,_L_,f Timothy P.JCieary /j Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN B. A. Wetzel, OPS 4A-SQN T. P. Cleary, OPS 4A-SQN G. R. Signer, WT 6A-K D. E. Pittman, BR 4T-C EDMS, WT CA-K (Enclosure) DMR0904.doc
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- APRIL DMR ORGANIZATION: Chemistry and Environmental DOCUMENT PREPARED BY: Ann Hurt DATE: 05/11/2009 CATEGORY: NPDES Correspondence CONCURRENCES Name R c Signature - Comment Date v N Ann Hurt X Stephanie A Howard X Alan K Barringer X Debra J Bodine X Beth A. Wetzel X Chris R Church X Timothy P Cleary X (for signature) INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 May 14, 2009 State ofT ennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR APRIL 2009 Enclosed is the April 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely, _./ 11 ~ (); e-J~Y"v ( rJ!.e-'\'-"-l Timothy~- Cleary I Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDE$) Form Approved. MAJOR Name_~VA-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Add res.§_ _E.~BOX_lgQQ_ _ _ _ _ _ _ _ _ _ _ _ _
---~NTEROFFICESB-2A-SQ~-------- TN0026450 101 G F- FINAL ---~D~DAISY_~~738L ______ _ PERMIT NUMBER DISCHARGE NUMBER] DIFFUSER DISCHARGE Facility_ _II/A -~EQUOYAH NUCLEAR PLANT_ _ _ _ _ _
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DAilYMX AME/TITLE PRINCIPAL EXEMIVE OFFJCE~;;fy under penalty of law that this document and all attachments were prepare.d under my rw . ..Qt' - (\ (I I ' ., . **c . d . ~I
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- - d1rect1on or superv1s1on 1n accordance w1th a system des1gned to assure that qual1f1ed personnel --:)11 f) lG:JL..-l.JL.lA '-L.I(f vz..._. A..\. , Timothy p Cleary Iproperly gather and evaluate the Information submitted. Based on my 1nqU1ry of the person or / * ""-"1' =
1 persons who manage the system, or those persons d~rectly responsible for gathenng the Principal Environmental Engineer 1 1nformat1on the 1nformat1on subm1tted IS . to the best of my knowledge and belief, true. 423 843- 6700 09 05 11 Sequoyah Site Vice President I accurate. and complete 1 am aware that there are s1gmf1cant penalt1esfor subm1ttmg false , SIGNATURE OF PRINCIPAL EXECUTIVE I ~..j 1
=rformat1on. 1ncludtng the possib1i1ty of f1ne and 1mpnsonment for know1ng v1olat1ons I OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY I TYPED OR PRINTED ____ CODE __:"j COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. The following information is included in an attachment: 1. CCW data 2. veliger monitoring data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2
DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 04/08/2009@ 1423 0.11 mg/1 04/10/2009 @ 0944 JOB TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 04/08/2009 ~ 1422 <0.10 mg/1 04/1 0/2009 @ 0954 JOB TN EPA 8015
Mean# of NOTES:% Mean# of Water Temp. Water Temp. LOCATION SUB Sample Date % Settlers ('C) Sample Date Asiatic Gravid Asiatic COLLECTED BY ZM/m3 ("C) LOCATION Clams/m3 Clam 1112112008 0 0 12 1111612008 230 12 lnplant RCW Dick Adcock 1112412008 31 0 11 1112412008 138 11 lnplant RCW Dick Adcock 121412008 0 0 10 1210412008 15 10 In plant RCW Dick Adcock 121812008 0 0 9 1210812008 0 9 In plant RCW Dick Adcock 1211512008 0 0 9 1211512008 0 9 In plant RCW Dick Adcock 1212212008 0 0 9 1212212008 0 9 In plant RCW Dick Adcock 1212912008 0 0 9 1212912008 0 9 In plant RCW Dick Adcock 0110912009 0 0 8 0110912009 0 8 In plant RCW Dick Adcock 0111612009 0 0 7 0111612009 0 7 In plant RCW Adcock I Bryant 0112212009 0 0 7 0112212009 0 7 lnplant RCW Adcock I Bryant 0113012009 0 0 9 0113012009 0 9 In plant RCW Adcock I Bryant 0210612009 0 0 6 0210612009 0 6 lnplant RCW Adcock I Bryant 0211312009 0 0 11 0211312009 0 11 In plant RCW Love 0212012009 0 0 9 0212012009 0 9 In plant RCW Bryant 0212612009 0 0 9 0212612009 0 9 lnplant RCW Bryant 03106/2009 0 0 8 0310612009 0 8 lnplant RCW Dick Adcock 0311312009 0 0 10 0311312009 0 10 In plant RCW Dick Adcock 0312712009 0 0 14 0312712009 0 14 lnplant RCW Wayne Wright 03130/2009 0 0 13 03/30/2009 0 13 In plant RCW Dick Adcock 04/09/2009 28 0 14 04/09/2009 0 14 In plant RCW Dick Adcock
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres_L __f_ ~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _
---~TEROFFICESB-2A-SQ~-------- TN0026450 ~ I 101 G F- FINAL
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persons who manage the system,or those persons directly responsible for gathenng the PrtnCipal Environmental Engineer ?OO I 1 1nformat1on, the 1nformat1on submitted 1S, to the best of my knowledge and bel1ef, tnue, 42 3 843-6 09 05 11 : Sequoyah Stte Vice President !accurate. and complete I am aware that there are sign1f1cant penalt1es for submttt1ng false SIGNATURE OF PRINCIPAL EXECUTIVE ___j____ _ j r-------- I
'Information, 1nciud1ng the poss1b1llty of f1ne and tmpnsonment for know,ng VIOlatiOns OFFICER OR AUTHORIZED AGENT AR~": 1 NUMBER YEAR MO Jl DAY ! , Tr:_PED OR PRINTE_r:J_ _ CODU ~
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 2 of 2
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~VA-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT !DMR) OMB No. 2040-0004 (SUBR 01) Addres.§_ ..£ ~BOX.1.QOO _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A-SQ~-------- TN0026450 101 T F-FINAL
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*' , Principal Environmental Engine~_r:____ 4 23 843-6700 I 09 05 11 Sequoyah Site Vice President accurate, and complete. I am aware that there are signtficant penalties for submitting false I ---- ~--
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PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMS No. 2040-0004 (SUBR 01) AddresL_E~BOX_filOO - - - - - - - - - - - - F- FINAL ---~TEROFFICESB-2A-SQ~-------- _ _ _ ___§_ODD...!:. : DAISY_ _IN 3738+/-..._ _ _ _ _ _ _ _ LOW VOL. WASTE TREATMENT POND Facility_ ~A -.§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio_12_ ...J::!AMIL TOl:!_COUNTL _ _ _ _ _ _ _ _ _ _ EFFLUENT DAY
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d1rect1on or superv1s1on 1n accordance w1th a system des1gned to assure that qualif1ed personnel properly gather and evaluate the 1nformat1on submitted Based on my 1nqU1ry of the person or persons who manage the system, or those persons d11ectly responsible for gathenng the 1nformat1on, the 1nformat1on subm1tted 1S, to the best of my knowledge and bel1ef. true. accurate, and complete 1am aware that there are s1gn1ficant penalt1es for submitting false
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1 1 The Turbine Building Sump (TBS) was discharged directly to the Yard Pond (YP) from 4/16@ 0319 to 4/17@ 1915. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~VA-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres.L___f~BOX_1900 - - - - - - - - - - - -
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PRINCIPAL EXECUTIVE OFFICER t Certify under penalty of law that thts document and all attachments were prepared under my dtrection or superviston tn accordance with a system designed to assure that qualified personnel \ . /\~1
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Timothy P Cleary properly gather and evaluate the tnformatton submttted. Based on my tnqutry of the person or I persons who manage the system, or those persons dtrect!y responsible for gathertng the I i Sequoyah Site Vice President tnformatton, the tnformation submttted ts, to the best of my knowledge and beltef, true, accurate, and complete ! am aware that there are s1gniflcant penalt1es for subm1tt1ng false Principal Environmental Engineer
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843-6700 09 I 05 11 I 1 I
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PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~VA-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 l (SUBR 01) Addre~~~BO~~----------- ---~TEROFFICESB~2A~SQ~-------- ~ TN0026450 .~ F- FINAL _ _ _ _20DD_'L: DAISY _ _Jf'U738'1._ _ _ _ _ _ _ _ _ __EERM[l NUMBEI RECYCLED COOLING WATER FacJl!!y_ _IYA ~_§.E:QUOYAJ::!.NUCLEAR PLANT _ _ _ _ _ _ Locatio~ _!jAMIL TOJ'LCOUNTY._ _ _ _ _ _ _ _ _ _ _ EFFLUENT DAY NO DISCHARGE I XX I *** 30 NOTE: Read instructions before completinq this form. GRAS,4 12 ioo4oo o o
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properly gather and evaluate the Information submttted Based on my 1nqu1ry of the person or . . . ~<J ~"f' 1 - "' persons who manage the system or those persons d:rectly respons:ble for gathenng the Principal Environmental Engineer 09 05 11
. . . 1nformat:on the 1nformat1on subm:lled :s to the best of my knowledge and belief true, 4 23 843 -6700 .
Sequoyah Site V1ce Pres1dent accurate, and complete I am aware that there are s:gn:f1cant penalt1es for subm1111ng false SIGNATURE OF PRINCIPAL EXECUTIVE I _ 1---~ 1 ~ . __J,nformat1on 1nclu01ng me poss:b1l1ty or f1ne and 1mpnsonment for know1ng V1olat1ons. 1 OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR! MO DAY TYPED OR PRINTED CODE 1
--~-------------*--
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Na~_~VA-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) (SUBR 01) OMB No. 2040-0004 Add res§_ ___E Q'"BOX.1_9QQ_ _ _ _ _ _ _ _ _ _ _ _ _
---~TEROFFICESB-2A-SQ~-------- [ TN0026450 110 T F- FINAL - _ _ ~ODDl_- DAISY _ _Ir:-!.]738~ _ _ _ _ _ _ _
Facility _ _:s;JA -_g::QUOY~NUCLEAR PLANT_ _ _ _ _ _ C . -....... . RECYCLED COOLING WATER Locatio.!}_ .JjAMIL TOJ::LCOUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard NO DISCHARGE I XX I *** NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION ----rNQ.--r~FREQUENCYrJ_S_A_M_P_L_El EX OF I TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE ANALYSIS i MAXIMUM UNITS I _______ j IIC25 STATRE ?DAY CHR iCERIODAPHNIA 23
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- d1rect10n or supervis1on tn accordance w1th a system des1gned to assure that qualified personnel ----- I DATE Timothy P. Cleary I 'I I ]properly gather and evaluate the 1nformat1on submitted. Based on my 1nqUify of the person or I !persons who manage the system. or those persons d~rectly responsible for gathenng the I I 11nformat1on the 1nformat1on subm1tted to the best of my knowledge and bel1ef. true, Principal Environmental Engineer accurate, a~d complete 1 am aware that there are slgn;f,cant penalt1es for subm1tt1ng false IS I Sequoyah Site Vice President 423 843-6700 09 05 I 11 MO~
_ ______________ tmat1on, 1nclud1ng the poss;bility of f1ne and 1mpnsonment for know1ng violations I SIGNATURE OF PRINCIPAL EXECUTIVE I L_ TYPED o~~INTE~-------- __ OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER iYEAR COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous may be used 1 of 1
PERMITIEE NAME/ADDRESS !Include Facilitv Name/Location 1f Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ MAJOR Form Approved. Name_~A~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) AddresL __£'_ ~BOX_1.900 _ _ _ _ _ _ _ _ _ _ _ _
---~TEROFFICESB-2A-SQ~-------- TN0026450 116 G F- FINAL - _ _ ~ODD...'f_- DAISY_ __It~!__;l738L _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER BACKWASH Fac_!l!!y_ ...2_VA ._g-:QUOYAH NUCLEAR PLANT_ _ _ _ _ _
Locatio!]_ _!:!AMIL TO!:!_COUNT:f_ _ _ _ _ _ _ _ _ _ _ MONITQRING PERIOD EFFLUENT I I DAY I (~Y-EAR I MO I ATIN: Stephanie A. Howard From YEAR 09 I MO 04 I 01 I Toj 09 I o4 I 30 DAY NO DISCHARGE CJ *** NOTE: Read instructions before completinQ this form I NO. I I PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION \FREQUENCY SAMPLE 1 EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS rBRIS, FLOATING (SEVERITY) ******** ******** ******** ******** 0 9A 0 AL I I 01345 1 0 0 VISUAL
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Timothy P. Cleary Sequoyah Site Vice President TYPED OR PRINTED I' Cerl1fy ~nder penally of law lhalth1s document and all attachments were pr~pared under my
.. d~recl1on or superv1s1on 1n accordance w1th a system des1gned to assure that qual1f1ed personnel
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~1nformat1 on. 1nciud1ng the possibility of f1ne and 1mpnsonment for know1ng Violations
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I DATE M~; 1 y i CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres.L_ _f ~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ ffiTEROFFICESB-2A-SQ~-------- TN0026450 ,-117-<[l F- FINAL - _ _ 20DD_'L- DAISY _ _Iri)738i_ _ _ _ _ _ _ _ PERMIT NUMBER ~ENUMBER] BACKWASH Facil1tv_ ..I:JA -..§EQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l_ .J+/-AMILTO..t!._COUNTY._ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT YEAR I DAY NO DISCHARGE [_] *** ATTN: Stephanie A. Howard From I 09 To 30 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS
- DEBRIS, FLOATING (SEVERrTY) SAMPLE ******** ******** ******** ********
MEASUREMENT 0 9A I I 101345 1 0 0 PERMIT REQUIREMENT IEFFLUENT GROSS VALUE i-*~---------- ----*--------~-----+-
***~ , """. u-lOlL AND GREASE VISUAL ******** 0 94 84066 0 0 EFFLUENT GROSS VALUE
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- NAME/TITLE PRINCIPAL EXECUTIVE OFFICER f--.-.---
Timothy P. Cleary
!I Cert1f 'under penalty of law that th1s document and all attachments were prepared under my d1rect1D nor superviSIOn in accordance with a system designed to assure that qualified personnel proper y gather and evaluate the Information submitted. Based on my inquiry of the person or /.;;~I .p t l(,UL.Lt LA(1 ij
.CJ:.t.{/ULA. .d TELEPHONE DATE I
person ; who manage the system, or those persons directly respons1ble for gathenng the Principal Environmental Engineer 1nform t1on, the informat1on submitted is , to the best of my knowledge and bel1ef, true, 423 843-6700 09 05 111 Sequoyah Site Vice President accura e, and complete l am aware that there are sign1f1cant penalties for submitting false I--SIGNATURE OF PRINCIPAL EXECUTIVE mform t1on, 1nc!ud1ng the poss1bil1ty of fine and 1mpr1sonment for knowing violations AREA r------------,=yp-ED OR PRINTED ___ OFFICER OR AUTHORIZED AGENT - NUMBER IYEAR MO I DAY
~ ____j___ 1 CODE 1 COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 3/99) Previous edit1ons may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Fac1litv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name_~VA~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres.L_ __E_.~BOX.1.QQQ_ _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A-SQ~-------- I TN002645-0 I 118 G F- FINAL - _ _ __§_ODD-Y_- DAISY _ _IN 3738!_ _ _ _ _ _ _ _ L_ PERMIT NUMBER ] DISCHARGE NUMBER WASTEWATER & STORM WATER Facility _ _JyA- SEQUOYA_I::!_NUCLEAR PLANT_ _ _ _ _ _ LocatioD_ _!:!AMILTO_ti_COUNT:I_ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT YEAR DAY NO DISCHARGE [XX] *** ATIN: Stephanie A. Howard From 09 30 NOTE: Read instructions before completinq this form SAMPLE TYPE
******** ******** 19 19
**** :::1 ;;,:~*****>:*.**.1~'100 ,c*~ MG/L :,. ~\JWtqEfh \(;RA8.
EFFLUENT GROSS VALUE <\!: .~ *. / ' :: <*; ;., oAtb?Mx * *:WEEK SOLIDS, SETTLEABLE SAMPLE ******** ******** ******** ******** MEASUREMENT 25 00545 0 0 W'KW'W'
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Timothy P. Cleary I "'" oo~ """'" "' Oo '""' '"" '"'"m'"' '"" "' "'""'m'"" ~** ". repared under my I .\'. :f-":~~ direction or superviSIOn In accordance With a system designed to assure that qua!rfled personnel properly gather and evaluate the ;nformallon subm;tted Based on my inqwy of the person or ot*, qJ'
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persons who manage the system, or those persons d~rectly responsible for gathenng the Principal Environmental Engineer i i 1-Myyl
,I . . . ;nformat;on. lhe ;nformat;on subm;tted ;s. lo the best of my knowledge and bel;ef, true, _ *-_::----- 423 843-6700 1 09 05 11 I I Sequoyah Site V1ce President accurate, and complete. I am aware that there are sigmf;cant penall;es for subm;ttmg false SIGNATURE OF PRINCIPAL EXECUTIVE I I I 1 ;nformat;on, ;nclud;ng the poss;bll;ty of f;ne and ;mpnsonment for Know;ng v;olat;ons I OFFICER OR AUTHORIZED AGENT AREA 1 NUMBER 1 YEAR MO i1 TYPED OR PRINTED j CODE __[________ _ _ _ ___l__ i COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfalL EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
S58 090611 800- NPDES CORRESPONDENCE June 11, 2009 State ofT ennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR MAY 2009 Enclosed is the May 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely, ~!~ Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN G. R. Signer, WT 6A-K T. P. Cleary, OPS 4A-SQN B. A. Wetzel, OPS 4A-SQN D. E. Pittman, LP 4B-C EDMS, WT CA-K (Enclosure) DMR0905.doc
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450 - MAY DMR ORGANIZATION: Chemistry and Environmental DOCUMENT PREPARED BY: Ann Hurt DATE: 06/04/2009 CATEGORY: NPDES Correspondence CONCURRENCES Name R c Signature -Comment Date v N Ann Hurt X u/7/o'J Stephanie A Howard X IP q oq Alan K Barringer X Debra J Bodine X Beth A. Wetzel X Chris R Church X Timothy P Cleary X
'TfC.. I I ~u.OO:,
(for signature) INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
Tennessee Valley Authority. Post Office Box 2000. Soddy Daisy, Tennessee 37384-2000 June 11, 2009 State ofT ennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR MAY 2009 Enclosed is the May 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. ~~f~ Ti~othy P-. tleary Site Vice President I Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Addres_L _£'. ~BOX_1_9QQ_ _ _ _ _ _ _ _ _ _ _ _ _
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- ;direction or supervls1on in accordance w1th a system des1gned to assure that qual1f1ed personnel TELEPHONE DATE
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"'""""and of my knowledge bel1ef. true,<M Principal Environmental Engineer Sequoyah Site Vice President . accurate, a~d complele. I am aware lhal there are s1gn1f1can1 penall1es for submitting false 423 843-6700 09 06 09 ~
* - - - - - - ~- 1nformallon, 1ncludmg the poss1bil1ty of f1ne and 1mpnsonment for know1ng VIOlations SIGNATURE OF PRINCIPAL EXECUTIVE I 0 ED OR PRINTED
-------------**- 1 OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
__j No closed mode operation. The following information is included in an attachment: 1. CCW data 2. Veliger monitoring data EPA Form 3320-1 (REV 3/99) of 2
DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 05/13/2009@ 1155 0.22 mg/1 05/14/2009@ 1644 CLS TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 05/13/2009@ 1200 <0.10 mg/1 05/14/09@ 1655 CLS TN EPA 8015
Mean# of Mean# of NOTES:% Sample Date Water Temp. Water Temp. LOCATION SUB ZM/m3 % Settlers ('C) Sample Date Asiatic Gravid Asiatic COLLECTED BY ("C) LOCATION Clams/m3 Clam 11/21/2008 0 0 12 11/16/2008 230 12 lnplant RCW Dick Adcock 11/24/2008 31 0 11 11/24/2008 138 11 In plant RCW Dick Adcock 12/4/2008 0 0 10 12/04/2008 15 10 In plant RCW Dick Adcock 12/8/2008 0 0 9 12/08/2008 0 9 In plant RCW Dick Adcock 12/15/2008 0 0 9 12/1512008 0 9 lnplant RCW Dick Adcock 12/22/2008 0 0 9 12/22/2008 0 9 In plant RCW Dick Adcock 12/29/2008 0 0 9 12/2912008 0 9 In plant RCW Dick Adcock 01/09/2009 0 0 8 01/09/2009 0 8 lnplant RCW Dick Adcock 01/1612009 0 0 7 01116/2009 0 7 lnplant RCW Adcock I Bryant 01/22/2009 0 0 7 01/2212009 0 7 lnplant RCW Adcock I Bryant 01/30/2009 0 0 9 01/3012009 0 9 In plant RCW Adcock I Bryant 02/06/2009 0 0 6 02/06/2009 0 6 In plant RCW Adcock I Bryant 02/13/2009 0 0 11 02/13/2009 0 11 In plant RCW Love 02/20/2009 0 0 9 0212012009 0 9 lnplant RCW Bryant 02/26/2009 0 0 9 02/2612009 0 9 lnplant RCW Bryant 03/0612009 0 0 8 0310612009 0 8 lnplant RCW Dick Adcock 03113/2009 0 0 10 0311312009 0 10 lnplant RCW Dick Adcock 0312712009 0 0 14 0312712009 0 14 lnplant RCW Wayne Wright 03/30/2009 0 0 13 0313012009 0 13 lnplant RCW Dick Adcock 04/09/2009 28 0 14 0410912009 0 14 In plant RCW Dick Adcock 05/06/2009 880 24 19 05/06/2009 21 19 In plant RCW CNW 05/12/2009 15091 0.3 20 05/12/2009 47 20 In plant RCW CNW 05/19/2009 3961 2 20 05/19/2009 445 20 In plant RCW BJ 0512712009 7696 0.8 22 05/27/2009 457 22 In plant RCW CNW
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Name_~A~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres_L ___£ Q_BOX_29Q_Q_ _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 G F- FINAL
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"!CHLORINE, TOTAL RESIDUAL ******** ******** ******** <0.018 0.042 19 0 27 I 31 GRAB
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!persons who manage the system, or lhose persons d~rectly responstble for gathenng the l~nformat1on the 1nformat1on subm1tted ts , to the best of my knowledge and belief, true, jaccurate. a~d complele 1 am aware lhat there are stgmftcant penalltesfor submittmg false
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I Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 i 843-6700 09 t 06 09 AREA I NUMBER IYEARL MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured: 1. H-150M (max. calc. cone. was 0.0446mg1L--Iimit 0.050mgiL) 2. H-150M (low detection level analytical method was <0.020mgll--limit 0.050mgiL) 3/99) Previous editions may be used Page 2 of 2
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES! MAJOR Form Approved. Name_~VA~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR! OMB No. 2040-0004 (SUBR 01) Addres.§_ ...£_ Q_BOX_19QQ_ _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 T F- FINAL
---~NTEROFFICESB-2A-SQ~-------- - _ _ ___§ODDY- DAISY_ _JI'::l_]7381._ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facility_ JYA-_§_[:QUOYAH NUCLEAR PLANT_ _ _ _ _ _
_hQcatiOD__ __!::!AMIL TOJ:!..COUNTY __________ _ MONITORING PERIOD EFFLUENT From YEAR I YEAR I MO DAy NO DISCHARGE LJ ... ATIN: Stephanie A. Howard 09 To 09 05 31 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUENCY I SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS iiC25 STATRE 7DAY CHR SAMPLE ******** ******** >100.0 ******** ******** 0 1 /180 I COMPOS MEASUREMENT 23 !CERIODAPHNIA 0 0 PERMIT iTRP3B REQUIREMENT !EFFLUENT GROSS VALUE rC2ssTATRE-7oA.v cHR-----~ SAMPLE ******** !PIMEPHALES MEASUREMENT i iTRP6C 1 0 0 PERMIT ** $EE" fc;:'OMP{:[q: ~FLUENT GROSS VALUE REQtJ]REMENJ J* ~~:.;~c l I i I I L -~ ~- -~- I rl -------------'------'-------~ I'direction
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[ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Cert1fy under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE
~ * * --*-- or superv1s1on 1n accordance w1th a system des1gned to assure that qualtfied personnel 1
Timothy P Cleary [properly gather and evaluate the Information subm1tted. Based on my inquiry of the person or persons who manage the system, or those persons directly respons1ble for gathenng the Principal Environmental Engineer
. . . J1nformation. the Information submitted 1s, to the best of my knowledge and belief, true, 423 843-6700 09 06 09 Sequoyah Site V1ce President accurate. and complete. 1 am aware that there are sign1f1cant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I '~-------
TYPED OR PRINTED i 1nformat1on. 1ncludmg the poss1b11tty of f1ne and 1mpnsonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled 5/10/09- 5/15/09. Report is attached. EPA Form 3320-1 {REV 3/99) Previous editions may be used Page 1 of 1
June 9, 2009 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, MAY, 2009 Per your request, I am only submitting an electronic copy of the subject report. The report provides results of compliance testing using fathead minnows and daphnids. Outfall101, samples collected May 10-15, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were> 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Call me at (256}386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment): Sherrard, R. M., PSC lX-C Files, ER&TA, CEB lB-M SQN May 2009m
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE
SUMMARY
Report Date: June 9, 2009
- 1. Facility I Discharger: Sequoyah Nuclear Plant I TVA
- 2. County I State: Hamilton I Tennessee
- 3. NPDES Permit#: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MOD): 1,579
- 6. Receiving Stream: Tennessee River CTRM 483.6)
- 7. 1Q10: 3,491
- 8. Outfall Tested: 101
- 9. Dates Sampled: May 10-15, 2009
- 10. Average Flow on Days Sampled (MOD): 1426, 1443, 1448
- 11. Pertinent Site Conditions: H-150M was injected into the ERCW B train beginning on 5/11/09@ 1220 through 5/14/09@ 1500.
- 12. Test Dates: May 12-19, 2009
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids ( Ceriodaphnia dubia)
- 15. Concentrations Tested(%): OutfalllOl: 11.3,22.6,45.2, 72.6,100 Intake: 100.0 Pimephales promelas: UV treated Outfall I 01: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0
- 16. PermitLimitEndpoint(%): Outfall101: IC2 5 =45.2%
- 17. Test Results: Outfall101: Pimephalespromelas: IC 25 > 100%
Ceriodaphnia dubia: IC25 > 100% UV treated Outfall 101: Pimephales pro me las: IC2s > 100% Page 1 of 107
- 18. Facility
Contact:
Ann Hurt Phone#: (423) 843-6714
- 19. Consulting I Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 22. Notes: Outfall101 samples collected May 10-15,2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were >
100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Minnow survival was significantly reduced when exposed to intake samples during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Page 2 of 107
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101, Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (oC) (mg/L) First Used By_ 05-10-09 0859 to 05-12-09 0950 101 05-11-09 1510 1.8, 1.8t <0.10 05-11-09 0759 05-13-09 0850 05-10-09 0933 to 05-12-09 0950 Intake 05-11-09 1510 1.3 <0.10 05-11-09 0833 05-13-09 0850 05-12-09 0821 to 05-14-09 0900 101 05-13-09 1433 3.2, 2.ot <0.10 05-13-09 0721 05-15-09 0853 05-12-09 0839 to 05-14-09 0900 Intake 05-13-09 1433 2.1 <0.10 05-13-09 0739 05-15-09 0853 05-16-09 0900 05-14-09 0828 to 101 05-15-09 1428 2.3, 1.5t <0.10 05-17-09 0920 05-15-09 0728 05-18-09 0903 05-16-09 0900 05-14-09 0842 to Intake 05-15-09 1428 1.7 <0.10 05-17-09 0920 05-15-09 0742 05-18-09 0903
*TRC =Total Residual Chlonne tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrivaL
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV -treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Page 3 of 107
Pimephales promelas Ceriodaphnia dubia Test Organisms:
- 1. Source: Aquatox, Inc. In-house Cultures
- 2. Age: 20.33 hours old <24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control I Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate: lQ l
- 6. Test Initiation: (Date/Time)
OutfalllOl 05-12-09 1220 ET 05-12-09 0950 ET UV Treated Outfall 101 05-12-09 1232 ET
- 7. Test Termination: (Date/Time)
Outfall 101 05-19-09 1123 ET 05-19-09 0854 ET UV Treated Outfall 101 05-19-09 1140 ET
- 8. Test Temperature: Outfall 101: Mean= 24.7°C Mean= 24.9°C (24.2- 25.1°C) (24.6- 25.2°C)
Test Temperature: UV-Treated Outfall101: Mean= 24.7°C (24.3- 25.0°C)
- 9. Physical I Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
Page 4 of107
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1. Results of a Pimephales pro melas Chronic/ 7-day Toxicity Test.
(Genus species) (Type I Duration) Conducted May 12 - 19, 2009 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used- days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 98 98 98 98 98 22.6% 100 100 100 100 100 98 98 45.2% 100 100 100 100 98 98 98 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 98 98 98 98 Intake 100 100 90 68 60 53 50 Mean Dry Weight (mg) Test Solutions (replicate number) (%Effluent) 1 2 3 4 Mean Control 0.730 0.684 0.895 0.761 0.768 11.3% 0.753 0.655 0.723 0.717 0.712 22.6% 0.654 0.723 0.673 0.760 0.703 45.2% 0.646 0.684 0.638 0.649 0.654 72.6% 0.665 0.632 0.656 0.603 0.639 100.0% 0.723 0.604 0.700 0.641 0.667 Intake 0.212 0.285 0.496 0.567 0.390 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Pennit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LCso: TUc = 1001IC2s Page 5 of 107
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type I Duration) Conducted May 12- 19, 2009 using effluent from Outfall 101. Percent Surviving Test (time interval used- days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate numbed (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 33 34 33 34 34 32 34 34 31 31 33.0 11.3% 33 36 32 32 36 33 33 34 33 32 33.4 22.6% 35 36 34 36 32 33 35 34 33 33 34.1 45.2% 36 34 31 35 35 33 33 34 33 31 33.5 72.6% 37 37 36 32 35 32 31 34 34 33 34.1 100.0% 39 38 32 36 34 33 34 35 34 31 34.6 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LCso: TUc = 100/ IC2s Page 6 of 107
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphnia dubia Chronic/7-day Toxicity Test.
(Genus species) (Type I Duration) Conducted May 12- 19, 2009 using water from Intake Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 33 35 33 35 34 33 31 31 33 33 33.1 Intake 39 35 41 36 35 38 37 36 37 34 36.8 IC2s Value: > 100% Calculated TU Estimates: < 1.0TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LCso: TUc = 100/ IC2s Page 7 of 107
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephales pro melas Chronic/ 7-day Toxicity Test.
(Genus species) (Type I Duration) Conducted May 12- 19, 2009 using effluent from UV Treated Outfa11101. Test Percent Surviving Solutions (time interval used- days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 98 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg) Test Solutions replicate number} (% Effluent) 1 2 3 4 Mean Control 0.637 0.743 0.695 0.659 0.684 11.3% 0.775 0.852 0.756 0.772 0.789 22.6% 0.737 0.727 0.706 0.653 0.706 45.2% 0.693 0.722 0.683 0.756 0.714 72.6% 0.682 0.668 0.665 0.722 0.684 100.0% 0.631 0.664 0.751 0.745 0.698 Intake 0.695 0.635 0.662 0.642 0.659 lC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LC5o: TUc = 100/ IC2s REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration Toxicant Results (IC2s) Pimephales promelas May 12 - 19, 2009 1208 7-days KCl 0.75 g/L Ceriodaphnia dubia May 05 - 12, 2009 0905 7 days NaCl 1.07 g/L Page 8 of 107
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for P imephalespromelas and Ceriodap~nia dubia Tests, Non~ treated SeqlJ()yalJ}'-l [l<.;]earPlant Outfull101 perfonned May 12-19,2009.
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Pimephales promelas 24.7 24.2 25.1 . i : : : c:erio~C/P~rlia ~ubic: ....... 24:9* . 2.~ 6. is:.:2. . . :::* :: : ): ..... .. .... .... : .:::::::::::- ::::: .. :::::. :::::::.*:: T_ ... :::::: :::: :::::* : :::::::::: .:*:: ::::.:::::. Page 9 of 107
[PHYSICAL/CHEMICAL
SUMMARY
........Y'!a,t~!..C.:l1~~tryJ11.~<111:Y.<l~L~~.<li1c1. ~!l~es.f.()~.f'.i~e:P.~.a.Zf!.spr.(J.l11.<?!C:~ ..!.~.~~~...LJY.~1!~<l~~c1.~.~q\l()Xa.~.~ll.c.l~~.?.~~~(~.Q~).9.1lt.fa:~..1.q1...
performed May 12-19, 2009. i**r* * -~*. ************-r*-r************** * ***********-*-;***r******* *- ****-r********* **r *:i***************:****************:****.*** ********-r*****-**** ***;***;************** Test Sample ID Temperature Cq Dissolved Oxygen (mg/L) ........................ . ~lk:llinity pH (S.U.) Conductance Hardness Initial Final Initial Final Initial Final (p.mhos/cm) (mg/L CaC03) (mg/L CaC03) 24.8 24.6 7.7 7.5 7.44 7.27 314 60 91 Control 24.7 - 24.9 24.4 - 24.9 I 7.5 > 7.8 7.0 :- 7.8 7.28 ;_ 7.53 7.o4:J 7.44 296 :_ 334 59 :_: 61 88 ) 92 24.8 24.6 7.7 7.3 ....... 7.45 7.25 302 11.3% 24.7) 25.0 24.4 - 24.8 7.5 :- 7.9 6.8 !- 7.5 7.29 :_ 7.53 7.08.LT 7.38 282 rr 321 TT ': **:*
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SUMMARY
I CONCLUSIONS Outfall 101 samples collected May 10-15, 2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were> 100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Minnow survival was significantly reduced when exposed to intake samples during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Page 11 of 107
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL o~ test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None Page 12 of 107
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
2 .. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B.
- 4. Dissolved oxygen was measured by SM 4500 0 G.
- 5. The pH was measured by SM 4500 H+ B.
- 6. Conductance was measured by SM 2510 B.
- 7. Alkalinity was measured by SM 2320 B.
- 8. Total hardness was measured by SM 2340 C.
- 9. Total residual chlorine was measured by ORION Electrode Method 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC 25 values in giL KCl orNaCl.
- 2. Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephales pro melas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 13 of 107
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Standard Methods for the Examination of Water and Wastewater, 20th Edition, 1998.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of 107
Sequoyah Nuclear Plant Biomonitoring May 12-19, 2009 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 107
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- May 15, 2009 Date PCL-401 mg!L Copolymer 03/12/1998 } 03/13/1998 ** 03/14/1998 : 03/15/1998 .** 03/16/1998 03117/1998 03/18/1998 09/08/1998 0.005 09/09/1998 0.011 09/10/1998 0.021 0911111998 0.019 09112/1998 0.015 9/13/1998 0.015 09/14/1998 0.015 0.024 0.024 0.024 0.024 0.024 0.024 0.023 Page 16 of 107
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- May 15, 2009 Date PCL-401 mg!L Copolymer 0.009 0.028 0.009 0.009 0.009 0.009 0.009 0.019 0.019 0.018 0.019 0.019 0.019 0.019 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.021 0.021 0.021 0.020 0.021 0.020 11/25/2001 11/26/2001 0.02 11/27/2001 0.019 11/28/2001 0.019 11/29/2001 ..* 0.02 11/30/2001 .. 0.02 12/09/2001 .* 12/10/2001 .*. *. 12/11/2001 .* 12/12/2001 . 0.02 12/13/2001
- 0.02 12/14/2001 *..* *.. 0.02 Page 17 of 107
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- May 15, 2009 Date PCL-401 mg/L Copolymer 01/02/2002 0.02 01/03/2002 0.014 01/04/2002 0.014 01/05/2002 01/06/2002 01/07/2002 0.014 02/24/2002 02/25/2002 0.023 02/26/2002 . 0.023 02/27/2002 0.023 02/28/2002 . 0.008 03/01/2002 .* 0.008 05/05/2002 05/06/2002 0.02 05/07/2002 0.02 05/08/2002 0.019 05/09/2002 0.02 05/10/2002 0.019 08/04/2002 . 08/05/2002 0.018 08/06/2002 .*. 0.018 08/07/2002 **.*.**.
- 0.019 08/08/2002 \ ... 0.019 08/09/2002
- 0.018 10/06/2002 *.*..
10/07/2002 *.. 0.018 10/0 8/2 002 !:..*<************ ********:*:c:.. *j****** 0.018 10/09/2002 ************************ 0.018 10/10/2002 .*.. 0.018 10/11/2002 .*.. 0.018 01/12/2003 ... 01/13/2003 0.019 01114/2003 *.** 0.020 01/15/2003 0.020 01/16/2003 .. 0.020 01/17/2003 :. 0.009 04/06/2003 .*. 04/07/2003 . . 0.021 04/08/2003 *.
- 0.021 04/09/2003 . 0.021 04/10/2003 0.021 04/ll/2003 . 0.022 Page 18 of 107
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998- May 15,2009 Date PCL-401 mg/L Copolymer 06/15/2003 06116/2003 0.020 06/17/2003 0.014 06/18/2003 0.014 06/19/2003 0.020 06/20/2003 0.020 08/03/2003 08/04/2003 0.020 08/05/2003 0.020 08/06/2003 0.020 08/07/2003 0.020 08/08/2003 0.020 10/05/2003 0.020 10/06/2003 0.020 10/07/2003 0.020 10/08/2003 0.020 10/09/2003 0.022 10/10/2003 0.024 02/01/2004 0.009 02/02/2004 0.009 02/03/2004 0.009 02/04/2004 0.009 02/05/2004 0.009 02/06/2004 0.009 05/04/2004 . 0.019 05/05/2004 *.. 0.014 05/06/2004 0.013 05/07/2004
- 0.020 05/08/2004 **.** . 0.021 05/09/2004 *..... 0.020 07/04/2004 . 0.019 07/05/2004 0.020 07/06/2004 0.020 07/07/2004 . 0.019 07/08/2004 r*\********************* 0.019 o7/09/2oo4 n. \. ?/ ?<>
N
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0 BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 ofl
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....... Client: TVA Environmental Testing Solution, Inc . Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comments(}~/W1YV~"7 Chevy Williams: Phone: 828-350-9364 Ben Mitchell : ~ ...{.1 __,. ~:;,... / NPDES Number: TN0026450 Adam Deimling: . / L.5x - Collected By: Chevy Williams, Ben Mitchell, Adam Deimling Fax: 828-350-9368 Metals Analyzed. Samples remained oiilce through out sampling and transport to lab. Field Identification I Grab/Comp. Collection Dateffime Container Flow Rain Event? ptmJul i. 544'-f Sample Description Number& MGD Laboratory Use Volume (Mark as Appropriate) Collected
" *~ . . / .*.. ::<,.' (~ .* ,5;~: Date Time
- n;::~*,-:~}*~*~:~*:~* *::*~~f:H* Yes If Yes, No Trace ETS Log Arrival By Time Appear-
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*. .*... ** ' ><. :'*'..:.*'.: > **.. . ,* P'..* .:;:> . < *.*. ,* ***c l**c:* :**
Inches Number (oC) t"' ance SQN-101-TOX Comp 05/10/09-05/11/09 0859-0759 2 (2.5gal) 14.)1, v O.D( ~DSlt.O} \.(fl.~ '(. ~ 6\0 ~ SQN-INT-TOX Comp 05/10/09-05/11/09 0933-0833 1 (2.5 gal) NA J 0 .ol 01~11-0Z- \.!)(_
~ lS'O Sample Custody - Fill In From Top Down 1f. ~to~ ~ ~o.cJ. "*'
Relinquished By (Signature): Date/Time Received By (Signature): I. cv.. 1tJ ~-~ ~1m~~n~ 0 ~ ChovyWil~~ 05/11/09
/tJRtGsr Sonic Delivery 05/ll/09 131<-. ~ 10.'~1 C\
Sonic Delivery/31<.. nvwl~ 05/11/09 05/11109 SLJ*f ETS 4 3: I D Ptl\ £:\ \S\0 t\
'-'I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
~ ~
IJQ It> N Vl 0 BIOMONITORING CHAIN OF CUSTODY RECORD Page 1oft
'""l 0 Delivered By (Circle One): -.1 Client: TVA Environmental Testing Solution, Inc.
Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General ~or:nnej0Jllut7j Chevy Wllhams: J_*~_,"~/dl{ J IL ..;;t~./ ;_,~ Phone: 828-350-9364 Ben Mitchell :-.2"'i NPDES Number: TN0026450 Adam Deimling: -~c:::::> y;;: Fax: 828-350-9368 Metals Analyzed. Samples remained on ice through out Collected By: Chevy Williams, Ben Mitchell, Adam Deimling sampling and transport to lab. Field Identification I Grab/Comp. Collection Daten'ime Container Flow PM)~ '544Y i Sample Description Number& MOD Rain Event? Laboratory Use Volume (Mark as Appropriate) Collected
/.~: ..*. ***.***
** . . *.** .: *****< . *.*** **:... ::. ...... Date Time ;:~;?**-*:~}:,;:~;), .;.~~*;:.i<*.;;*, Yes If Yes, No Trace ETSLog Arrival By Time Appear*
Temp.
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*., : :*:*c.? l,x.=rz)!; Inches Number c*c) E' ance SQN-101-TOX Comp 05/12/09-05/13/09 0821 -
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c'j~ Chevy WimazruC4 ~ 05/13/09 10 55 6srr Sonic Delivery 05/13/09 C)l r&-+' j3/t ~ ;0:33 'E,\ Sonic Delivery fJ_ fl _U4 05/13/09 1_: :is PM E\ ETSn~l~ 05/13/09 L'-1~3> E:\
'-../ I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not anive until the following Monday.
~---------~------ ---
~
llol (JQ
~
N 0'1 0 BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 I Q Client: TVA Delivered By (Circle One): i
-...:a Environmental Testing Solution, Inc.
Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery g:.:~=~~ Facility Sampled: Sequoyah NP 28801 NPDES Number: TN0026450 Phone: 828-350-9364 B*n M>tchell ' : Adam Deirnling: Collected By Chevy Williams, Ben Mitchell, Adam Deimling Fax: 828-350-9368 Metals samples taken. Samples remained on ice through out sampling and transport to lab. Field Identification I GYL/~ Grab/Comp. Collection Dateffime Container Flow Sample Description Number& MGD Rain Event? Volume (Mark as Appropriate) ~# Laboratory Use Collected
,'< Date Time ~: :/~' .. **;:~: Yes If Yes, No Trace ETS Log Arrival By Time Appear-Inches Number Temp. MCe E:\ > ' ' :.*'- - f:l
. ("C)
SQN-101-TOX Comp 05/14/09-05/15/09 0828- 2 (2.5gal) 0728 14-4<3:3 / o.olt ~!$-Cll-2..~}\.S'L J l~'l.t j,.. 0842-SQN-INT-TOX Comp 05114/09-05/15/09 0742 I (2.5 gal) NA v O.DL\ 01U51S.D3 \:l"L a\ *~-za 1k Sample Custody- Fill In From Top Down -i. {.w,\~ -~ ~-~-
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I 11-_A Allrn .AKd.. ~~~r-n., Relinquished By (Signature): Date/Time Received By (Signature): ,_. - D !Time ..M. .......... ("] Chevy Williams 05115109 Sonic Delivery 05/15/09
'J~ ~ !Oo117sr B!< :u~£- j[)~' f)q_
I E.\ Sonic Delivery{? tt> ~ 05/15/09
!:2; 2 r fl'\ E.'T" ETS rl~-
05/15/09 t'luf ~\
\...J v Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
--~---------
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Page 1 of6 I I
~
~ ~
EnYitonmentilflating SoluUans, lilt. I I Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) 1 Species: Pimephales promelas Client: TVA County: Hamilton I Facility: Seguoyah Nuclear Plant Treatment: Non-treated Ij NPDES #: TN 0026450 Project #: --.,-~::::.~-.....:'-'....;'-\!,_~.1...,_---- Outfall: 101 I j Dilution preparation information: Comments: I. j - Dilution prep(%) Effluent volume (mL) 11.3 282.5 22.6 565 45.2 1130 72.6 1815 100 2500 l Diluent volume (mL) Total volume (mL) 2217.5 2500 1935 2500 1370 2500 685 2500 0 2500 I! Test organism information: Test inf!Jrmation: l Organism age: "2o * ~:, \I.Quv'l':> 0'-~ Randomizin__g tem_Q}ate: l~11 nu'l Date and times organisms 0~, \- o'\ \loCO Incubator number and were born between: shelf location: 6C.. I~ Organism source: Transfer bowl information: pH= fl>..'Th'l- ~P\rt.,t\ p P C'S* \\*d'\ SU Temperature = oc Artemia CHM number: o\1-lt..\"\~ Date I Time in: ~ *\"'*o'\ J'l.()b 1
,.~'1.. '"t'-t.l..:. Date I Time out: OS*lJ:I*cf ll."tt Average transfer volume: Total drying time: uf.tS~b.
- o. 1.:!:.oS ~ Initial oven temperature: ~t)'L Final oven tem_Qerature: ~\'{,....
Daily feeding and. renewal information: Day Date Morning feeding Afternoon feeding Sample MHSW numbers used batch used 0 2 3 4 5 6 I 7
!I I
Page 27 of 107
~ 2 ~
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Page 2 of6
) .
~ EnvlrontntAt11Testtng.$olutloru,lnc.
Species: Pimephales promelas Date: OS*'~ o~ Client: TVA I Sequoyah Nuclear Plant- Non-treated Survival and Growth Data Day t.;UN.fROL 11.3% 22.6% A B c D E F -G 8 I J K L 0 ID ID /Q 10 /D 10 ID IO 10 /0 10 10 1 ro 10 ID IO to 10 IO IO IQ /0 IO 10 2
/Q ro 10 ID /f) IO 10 1(:) lf::J fQ /(:) tO 3
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Species: Pimephales promelas Date: _0:.:::5...__,_*1_,_"2_,*o~~-=------ Client: TVA I Sequoyah Nuclear Plant- Non-treated Survival and Growth Data Day 100% Intake y z AA BB 0 10 ro 10 10 1
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Not for Compliance Assessment, Internal Laboratory QC ( J Coauatratioa (%) Repliac.e hitiaiDD.mberof Fiaal a umber of A'"" Pa.a weight B-ha+l...arue Larvae weigbt (mg) Weigllt/ Survivia, Malawc::i.ptl Codllideat ofvariatioo Wcipt/ Jaitial . . mber Mc-.auannival Meuweipt/ ""eddlck.otol Pcrt.c~~.t rod.adioa &0111 larv11c larvae (") weigb t ( rng} *A-B umber of larvae (Mg.) Swnrivi*C aumber or (Mft*WacJiolpa .... YiYia' afbln .. (mg) (Yo) l*itial aamber at' variaD.oa (M- wnp1 COII.lTOt(o/*} larv.. (mg} naberafl*"*e>("!.) llllVIOC(mg) pwlllid .. au.beroftarne) A 10 10 14.87 22.17 7.30 0.730 0730 B 10 10 13 86 20 70 6.84 0.684 0.684 Control 0.768 11.8 100.0 0.768 11.8 Not applicable c 10 10 15 01 23 96 8 95 0&95 0.&95 D 10 10 12 40 20.01 7.61 0.761 0.761 E 10 10 14.50 22.03 7.53 0.753 0.753 F 10 9 13.70 20.25 6.55 0.728 0.655 11.3% 0.730 2.2 97.5 Cl.71l 5.8 7.l G 10 10 13.68 20.91 7.23 0.723 0.723 H 10 10 14.50 21.67 7.17 0.717 0.717 I 10 10 13.91 20.45 6.54 0 654 0.654 J 10 9 13.27 20.50 7.23 0.803 0.723 22.6'Vt 0.723 9.8 97.5 Cl.703 6.9 8.5 K 10 10 15.13 21.86 6.73 0.673 0.673 L 10 10 13.90 21.50 7.60 0.760 0.760 M 10 10 l3S9 20.35 6.46 0.646 0.646 N 10 10 13.97 20.81 6.84 0.684 0.684 45.2% 0.672 4.5 97.!1 0.654 3.1 14.8 0 10 9 13 70 20.08 6.38 0.709 0.638 p 10 10 14.08 20.57 6.49 0.649 0.649 Q 10 10 1372 20 37 6.65 0.665 0.665 R 10 10 15.75 22.07 6.32 0 632 0.632
'72.6o/o 0.639 4.3 lOCI.O Cl.639 4.3 16.7 s 10 10 14.24 20.80 6.56 0 656 0.656 T 10 10 14 28 20 31 6.03 0 603 0.603 u 10 10 14 94 22 17 7.23 0.723 0.723 i
100 1% v 10 9 15 87 21 91 6.04 0.671 0 684 5.2 0 604 97.5 0.667 8.2 13.1 w 10 10 14 33 21 33 7.00 0.700 0 700 X 10 10 13.69 20 10 6.41 0.641 0 641 y 10 3 13.79 15 91 2.12 0.707 0212 JOO'/* Jnmke z 10 4 14.14 16.99 2.85 0.712 0.764 8.3 0.285 50.0 0.390 43.2 49.1 AA 10 6 14.85 19 81 4 96 0.827 0.496 BB 10 7 13.49 19.16 5.67 0.&10 0 567 OutfaliiOl: MSD- Minimum Significant Difference Dunnett's MSD value: 0.0890 PMSD- Percen1 Minimum Significant Difference PMSO: 11.6 PMSD tS a measure oftest precision. The PMSD is the minimum percent d1fference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test In tal<<: Dunnett's MSD value: 0.1860 Lower PMSD bound determined by USEPA (lOth percentile) ~ 12%. PMSO: l4.2 Upper PMSD bound determined by USEPA (90th percentile)- 30%. Lower and upper PMSD bounds were detenruned from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200la; USEPA, 200lb). USEPA 200 I a, 200 lb. Fmal Report. Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-{) l-{)04 and EPA-821-B-0 1-{)05 US Environmental Protection Agency, Ctncmnati, OH
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Larval Fish Growth and Survival Test-7 Da}': Growth Start Date: 5/12/2009 TestiD: PpFRCR Sample ill: TVA I Sequoyah Nuclear Plant, Outfall 101 End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-0 13 Test Species: PP-Pimephales promelas Comments: Cone-% l 2 3 4 D-Control 07300 0.6840 0.8950 0.7610 11.3 0.7530 0.6550 0.7230 0.7170 22.6 0.6540 0.7230 0.6730 0.7600 45.2 0.6460 0.6840 0.6380 0.6490 72.6 0.6650 0.6320 0.6560 0.6030 100 0.7230 0.6040 0.7000 0.6410 Intake 0.2120 0.2850 0.4960 0.5670 Transform: Untransformed !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7675 1.0000 0.7675 0.6840 0.8950 11.817 4 0.7675 1.0000 11.3 0.7120 0.9277 0.7120 0.6550 0.7530 5.777 4 1.502 2.410 0.0890 0.7120 0.9277 22.6 0.7025 0.9153 0.7025 0.6540 0.7600 6.851 4 1.760 2.410 0.0890 0.7025 0.9153
*45.2 0.6543 0.8524 0.6543 0.6380 0.6840 3.113 4 3.066 2.410 0.0890 0.6543 0.8524
*72.6 0.6390 0.8326 0.6390 0.6030 0.6650 4.343 4 3.479 2.410 0.0890 0.6530 0.8508
*100 0.6670 0.8691 0.6670 0.6040 0.7230 8.152 4 2.721 2.410 0.0890 0.6530 0.8508 Intake 0.3900 0.5081 0.3900 0.2120 0.5670 43.230 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97087646 0.884 0.63537989 1.21808683 Bartlett's Test indicates equal variances (p = 0.23) 6.92620468 15.0862722 Hypothesis Test (!-tail, 0.05)__ NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 22.6 45.2 31.9612265 4.42477876 0.08902763 0.11599692 0.00884297 0.00272926 0.0292745 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamp1es)
Point % SD 95% CL(Exp) Skew
!COS* 7.813 ICIO 28.104 IC15 >100 IC20 >100
!C25 >100 IC40 >100 lC50 >100
- indicates IC estimate less than the lowest concentration sqn101_05-12-09data
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Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 5112!2009 Test ID: PpFRCR Sample ID: TV A I Sequoyah Nuclear Plant, Outfall 101 - Intake End Date: 5119/2009 Lab 10: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-0 13 Test Species: PP-Pimephales promelas Comments: Cone-% 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 11.3 1.0000 0.9000 1.0000 1.0000 22.6 1.0000 0.9000 1.0000 1.0000 45.2 1.0000 1.0000 0.9000 1.0000 72.6 1.0000 1.0000 1.0000 1.0000 100 1.0000 0.9000 1.0000 1.0000 Intake 0.3000 0.4000 0.6000 0.7000 Transform: Arcsin Sguare Root 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 11.3 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 22.6 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 45.2 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 72.6 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 100 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4
*Intake 0.5000 0.5000 0.7854 0.5796 0.9912 23.814 4 6.701 2.353 0.2201 Auxili~ Tests Statistic Critical Skew Kurt Shapii:o-Wilk's Test indicates normal distribution (p > 0.01) 0.93208891 0.749 3.38354E-16 0.88161324 Eguali!}: of variance cannot be confirmed H~othesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Heteroscedastic t Test indicates significant differences 0.11179766 0.11466427 0.78530011 0.01749093 5.4E-04 l, 6 Treatments vs D-Control sqnl 01_05-12-09data
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Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Growth and Survival Test-7 Da~ Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: TV A I Sequoyah Nuclear Plant, Outfall I 0 I -Int~ke End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% I 2 3 4 D-Control 0.7300 0.6840 0.8950 0.7610 11.3 0.7530 0.6550 0.7230 0.7I70 22.6 0.6540 0.7230 0.6730 0.7600 45.2 0.6460 0.6840 0.6380 0.6490 72.6 0.6650 0.6320 0.6560 0.6030 100 0.7230 0.6040 0.7000 0.6410 Intake 0.2120 0.2850 0.4960 0.5670 Transform: Untransformed !-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.7675 1.0000 0.7675 0.6840 0.8950 11.8I7 4 11.3 0.7120 0.9277 0.7120 0.6550 0.7530 5.777 4 22.6 0.7025 0.9153 0.7025 0.6540 0.7600 6.851 4 45.2 0.6543 0.8524 0.6543 0.6380 0.6840 3.113 4 72.6 0.6390 0.8326 0.6390 0.6030 0.6650 4.343 4 100 0.6670 0.8691 0.6670 0.6040 0.7230 8.152 4
*Intake 0.3900 0.508I 0.3900 0.2120 0.5670 43.230 4 3.944 1.943 0.1860 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94991392 0.749 0.13317371 -1.3590747 F-Test indicates equal variances (p = 0.34) 3.45560646 47.4672279 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.18600391 0.24235037 0.2850125 0.01832517 0.00759199 1, 6 Treatments vs D-Control sqnl01_05-12-09data
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Control 11.3% 22.6% 45.2% 72.6% 100%
~ETS Page 5 of6
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Species: Pimephales promelas Client: TV A I Sequoyah Nuclear Plant- Non-treated 11.3% 22.6% 45.2% 72.6% 100% 100% Intake Page 36 of 107
Page 6 of6 Species: Pimephales promelas Date: cS* 1-z. =o'1 Client: TV A I Sequoyah Nuclear Plant- Non-treated Coocen- Parameter tration 11.3% 22.6% 45.2% 72.6% 100% lOOo/e Intake Page 37 of 107
~ETS Page 1 of7
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Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA County: Hamilton Facility: Seguoyab Nuclear Plant Treatment: Non-treated NPDES #: TN 0026450 Outfall: 101 Project#: ____,S~-4'-\-!.....!l.\_ _ _ __ Dilution preparation information: Comments: Dilution prep(%) 11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test orKanism source information: Test information: Organism age: < 24-hours old Randomizing template color: l&l'\E> Date and times organisms were born OS* l"l.*cf\ Olo~ ""tO CAt.\ 0 Incubator number and shelf between: location: 2&\ Culture board: 05*0")yt:1\. A Replicate number: 1 I z I 3 1 4 1 s 1 6 1 1 1 s 1 9 1 10 YWTbatch: Culture board cup number: ~IS I IOI\CS.II'ol 1'"\l\'\ l1..1.l'l.'!ti1.S ol.\*zt-<A Transfer bowl information: pH- *v\~ su Temperature= "Z.'-l.'\ oc Selenastrum batch: to~ -C:>'\* tt\ Daily renewal information: Day Date Test initiation, MHSW Sample numbers Analyst batch used used 0 2 3 4 5 6 7 Control information: 1 2 Acceptance criteria Summary of test endpoints:
% of Male Adults: 07. 07. !>20% 7-day LCSO -, ICOi.
%Adults having 3ra Broods: ;oo7. IOQ7. ~80% NOEC 1ao'.
% Mortality: 07. 01. !>20% LOEC >mc*r.
Mean Offspring/Female: ~.0 -~*\ ~ 15.0 offspring/female ChV '>tocr.
%CV: 3.~7- '4. \7 <40.0% IC25 ) 1001~
Page 38 of 107
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Page 2 of7
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Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant- Non-treated Date: CONTROL-I Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L- L \..._ \...._ '- '- '- L 1._ '- 2 Young produced [) 0 0 0 0 0 () () a 0 Adult mortality L ' - '-- '-- '- \.,_ \...._
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'- \.._ L '-- '-- L \.._ '- L 7 Young produced \\.. 1\ \'5 \& \~ \l.o \'5 \\ \'S IS Total young produced
~~ :M ~:, o'-\ ~"\ o'2. ~'-\ ~'-\ ,:,\ ~\
Final Adult Mortality '- '-- '- \._ \......... L '- \....... L '-- X for yd Broods y...... )'C- )<. ')<... ~ >C y -..,L ~ Y-Note: Adult mortality (L = hve, D =dead) CONC: 11.3% Survival and Reproduction Data Replicate number Day_ 1 2 3 4 5 6 7 8 9 10 1 Young produced () () 0 0 0 n 0 0 0 0 Adult mortality L- L L L L L L L '- L 2 Young produced 0 0 D 0 0 0 C) () 0 0 Adult mortality L L \.._ L L L L L l_ I.... 3 Young produced D a D 0 0 0 0 0 a 6 Adult mortality L \..._ \.._ '--- L L L L L L 4 Young produced ~ 5 "S 'S lo y Ll L{ ~ '-\ L Adult mortality L L \...._ L L L L L L 5 I'-\ 1:::0 \~
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- ,~ 3<c. :*z... 3'2_ 31.:. ¢:, 3:, 3'-\ .3~ ~"2..
Final Adult Mortality \._ '-- '--- '--- '-- '-- '--- '- L '--- Note: Adult mortality (L = hve, D = dead) Concentration:
% Mortality: ()1.
Mean Offspring/Female: a~.!.\
% Reduction from Control: - \.?... '1 ;>
Page 39 of 107
~ETS Page 3 of7
~ EnvtronmentaiTe:ltln§Sohltlons.lnc.
Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant- Non-treated Date: _..::;OS_*_l'l_*cf\ CONC: 22.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0
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% Mortality: (')1.
Mean Offspring/Female: ..34.\
% Reduction from Control: - ~.~7.
CONC: 45.2% Survival and Reproduction Data Replicate number Day l 2 3 4 5 6 7 8 9 10 1 Young produced L) 0 0 0 0 0 0 0 0 0 Adult mortality '\.__
'- \.__ \..__ \._ L L. L L L 2 Young produced 0 0 0 0 a a 0 0 0 D Adult mortality
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Note: Adult mortality (L =live, D =dead) Concentration:
% Mortality: 01.
Mean Offspring/Female: ~.s
%Reduction from Control: - \.Si.
Page 40 of 107
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Envlnlnm.nt.al Testing Sotutlons. )nc. Page 4 of7 Species: Ceriodaphnia dubia Client: Seguoyab Nuclear Plant- Non-treated Date: o5-\'l-Q'\ CONC: 72.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced () (') a 0 0 D 0 0 0 0 Adult mortality
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6 Young produced 0 0 0 0 0 0 0 CJ 6 0 Adult mortality L.. '-- '-- L \.__ L l._ '- \._ L 7 Young produced \g, \& \\,:, l"-\ \IS \S \'-\ l\ l\o \~ Total young produced
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Final Adult Mortality \ \...... \.._.. \..._ '-- '-- \.,._ \..._ '- Note: Adult mortality (L =live, D =dead) Concentration:
% Mortality: D't.
Mean Offspring/Female: a~.{.'
%Reduction from Control: * ?l-37.
CONC: 100% Survival and Reproduction Data Replicate number Day I 2 3 4 5 6 7 8 9 10 I Young produced Adult mortality 0 L () L- L 0 0 L 0 L L 0 () L- ,__0 0 L L 0 2 Young produced () 0 0 0 0 0 C) 0 0 0 Adult mortality
'- \.._ \..._ '- '- L_ L L- L L 3 Young produced () () (_) 0 0 0 0 0_ 0 0 Adult mortality L L '- L L L L '- L L 4 Young produced '$ lo ~ 'S s s ~ ~
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Adult mortality L '- \._ L L \._ L \.._ L I.... 5 Young produced \S 1..1. 1"2.. 1'-\ \~ 1'-\ \~ \~ \~ \2_ Adult mortality ~ L L L L L c._ 1...._ L- L_ 6 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality \._.. L L L_ L L L L L L-7 Young produced \'\ \r \\.::. \\ \\o \'-\ \\ \\o \~ \S Total young produced
- f\ ~ ~2 ~lo 3'-\ ~~ 3'-\ 3S 3.1.\ 3\
L \.._
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Note: Adult mortality (L =live, D =dead) Concentration:
% Mortality: 07.
Mean Offspring/Female: ~~-lo
%Reduction from Control: - '-\, ~'7~
Page 41 of I 07
Page 5 of7 Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant- Non-treated Date: OS-1'7..--ot\ CONTROL-2 Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 D () 0 0 0 (j a (\ 0 Adult mortality L- L- L '-- L- L L L '-- '--- 2 Young produced 0 0 0 0 D 0 0 0 0 Q Adult mortality L L L '--- L L L. L L \.___ 3 Young produced {"') 0 a 0 0 D 0 0 0 a Adult mortality \._ \_. L- L '--- \._ L L \._. L 4 Young produced L\ '-'< '-\ ~ 'S '-\ s '-\. '-\ "'\ Adult mortality \_ \.._ L L. L. '--
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Final Adult Mortality '- '- '- L. L. '-- L L L L. Note: Adult mortality (L = live, D = dead) coNe: 100% Intake Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced (.) 0 0 (} (') 0 0 0 0 0 Adult mortality \._. \..._ L- L L L L L L L 2 Young produced 0 0 aL 0 () L__ 0 0 0 0 0 Adult mortality L L- '-- L__ L. '-- L L. 3 Young produced () 0 0 0 0 0 0 a 0 0 Adult mortality
'-- L \.__ \.._ L L L L '- \..._
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% Mortality: Q't.
Mean Offspring/Female: 31o.~
% Reduction from Control: -li.Z1o Page 42 of 107
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CJC< TVA I Sequoyah Nuclear Plant, OutfalllOl- Non-treated
- ~ETS ~ .j;o.
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..... Verification of Ceriodaphnia Reproduction Totals Q -..J ..J Environmental Te$tlng Solutions, Inc.
Control-! 72.6% Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 {) 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 5 5 5 5 4 4 45 4 6 4 5 5 5 4 4 4 4 4 45 5 13 13 13 12 13 II 14 12 12 12 125 5 13 15 15 13 12 13 13 13 14 13 134 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 {) 0 7 16 17 15 18 16 16 15 17 15 15 160 7 18 18 16 14 18 15 14 17 16 16 162 Total 33 34 33 34 34 32 34 34 31 31 330 Total 37 37 36 32 35 32 31 34 34 33 341 11.3% 100% Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 5 5 6 4 4 4 4 4 45 4 5 6 4 5 5 5 4 6 6 4 50 5 12 14 12 13 13 13 13 14 13 13 130 5 15 14 12 14 13 14 13 13 13 12 133 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 17 15 14 17 16 16 16 16 15 159 7 19 18 16 17 16 14 17 16 15 15 163 Total 33 36 32 32 36 33 33 34 33 32 334 Total 39 38 32 36 34 33 34 35 34 31 346 22.6% Control-2 Replicate number Replicate number Day Total Day Total I 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 6 6 4 4 5 4 5 5 48 4 4 4 4 4 5 4 5 4 4 4 42 5 14 13 13 14 ll 14 12 13 13 13 130 5 14 12 13 13 13 13 12 lO 14 12 126 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 19 15 16 17 15 18 17 15 15 163 7 15 19 16 18 16 16 14 17 15 17 163 Total 35 36 34 36 32 33 35 34 33 33 341 Total 33 35 33 35 34 33 31 31 33 33 331 45.2% 100% Intake Replicate number RePlicate number Day Total Day Total i I 2 3 4 5 6 7 8 9 10 l 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 I* 3 0 0 0 0 {) 0 {) 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 i 4 5 4 5 5 5 5 5 5 4 4 47 4 5 4 6 4 4 5 5 5 5 4 47 I 5 15 12 12 13 13 12 13 14 14 12 130 5 15 12 14 14 12 13 14 13 13 13 133 ] 6 0 0 0 0 0 0 {) 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 i 7 16 18 14 17 17 16 15 15 15 15 158 7 19 19 21 18 19 20 18 18 19 17 188 Total 36 34 31 35 35 33 33 34 33 31 335 Total 39 35 41 36 35 38 37 36 37 34 368 J
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/I Concentration Replicate number Survival Average reproduction Coefflcien( of Percent reduction from
(%) (o/o) (offspring/female) variation (%) pooled controls ('Yo) 1 2 3 4 5 6 7 8 9 10 Control- 1 33 34 33 34 34 32 34 34 31 31 100 33.0 3.8 Not applicable 11.3% 33 36 32 32 36 33 33 34 33 32 100 33.4 4.5 -1.2 22.6% 35 36 34 36 32 33 35 34 33 33 100 34.1 4.0 -3.3 45.2% 36 34 31 35 35 33 33 34 33 31 100 33.5 4.9 -1.5 72.6% 37 37 36 32 35 32 31 34 34 33 100 34.1 6.3 -3.3 100% 39 38 32 36 34 33 34 35 34 31 100 34.6 7.2 -4.8 Control- 2 33 35 33 35 34 33 31 31 33 33 100 33.1 4.1 Not applicable 100% Intake 39 35 41 36 35 38 37 36 37 34 100 36.8 5.7 -11.2 OutfalllOl: MSD= Minimum Significant Difference Dunnett's MSD value: 1.831 PMSD= Percent Minimum Significant Difference PMSD: 5.5 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test ~ Dunnett's MSD value: 1.374 Lower PMSD bound determined by USEPA (lOth percentile) = 13%. PMSD: 4.2 Upper PMSD bound determined by USEPA (90th percentile) = 4 7%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200la; USEPA, 200Ib). USEP A 2000. Understanding and Accounting for Method Variability in Whole Effiuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEP A 200 I a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2-Appendix. EPA-82l*B-O 1-004 and EPA-821-B-0 1-005. US Environmental Protection Agency, Cincinnati, OH.
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--.1 May 12-19, 2009 Statistical Analyses
* ) Environmental Testing Solutions, Inc.
Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/12/2009 Test ID: CdFRCR Sample ID: TV A I Sequoyah Nuclear Plant, Outfall I 01 End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R~2-0l3 Test Species: CD-Ceriodaphnia dubia Conunents: Cone-% 2 3 4 5 6 7 8 9 10 Control-! 33.000 34.000 33.000 34.000 34.000 32.000 34.000 34.000 31.000 31.000 Control-2 33.000 35.000 33000 35.000 34.000 33.000 31.000 31.000 33.000 33.000 11.3 33.000 36.000 32.000 32.000 36.000 33.000 33.000 34.000 33.000 32.000 22.6 35.000 36.000 34.000 36.000 32.000 33.000 35.000 34.000 33.000 33.000 45.2 36.000 34.000 31.000 35.000 35.000 33.000 33.000 34.000 33.000 31.000 72.6 37.000 37.000 36.000 32.000 35.000 32.000 31.000 34.000 34.000 33.000 100 39.000 38.000 32.000 36.000 34.000 33.000 34.000 35.000 34.000 31.000 Intake 39.000 35.000 41.000 36.000 35.000 38.000 37.000 36.000 37.000 34.000 Transform: Untransformed !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean Control- I 33.000 0.9970 33.000 31.000 34.000 3.779 10 33.783 1.0000 Control-2 33.100 1.0000 33.100 31.000 35.000 4.140 lO 11.3 33.400 1.0091 33.400 32.000 36.000 4.508 10 -0.500 2.287 1.831 33.783 1.0000 22.6 34.100 1.0302 34.100 32.000 36.000 4.019 10 -1.374 2.287 1.831 33.783 1.0000 45.2 33.500 1.0121 33.500 31.000 36.000 4.925 10 -0.624 2.287 1.831 33.783 1.0000 72.6 34.100 1.0302 34.100 31.000 37.000 6.252 10 -1.374 2.287 1.831 33.783 1.0000 100 34.600 1.0453 34.600 31.000 39.000 7.235 10 -1.998 2.287 1.831 33.783 1.0000 Intake 36.800 1.1118 36.800 34.000 41.000 5.700 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.653808415 1.035 0.254140335 -0.2360760 I Bartlett's Test indicates equal variances (p = 0.26) 6.457315922 15.08627224 The control means are not significantly different (p = 0.87) 0.170664037 2.100922029 Hypothesis Test (!-tail, 0.05) NOEC WEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 I 1.83092058 0.055482442 3.416666667 3.205555556 0.389506936 5, 54 Treatments vs Control-! Linear Interpolation (200 Resamples) Point % SD 95%CL Skew IC05 >100 JCIO >100 ICI5 >100 IC20 >100 IC2S >100 IC40 >100 1C50 >100 sqnl 01_05-12-09data
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Environmental Testing Solutions, Inc. Statistical Analyses Ceriodaplmia Survival and Reproduction Test-Reproduction Start Date: 5112/2009 Test ID: CdFRCR Sample ID: TVA I Sequoyah Nuclear Plant, Outfalll01 -Intake End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaplmia dubia Comments: Cone-% I 2 3 4 5 6 7 8 9 10 Control-! 33.000 34.000 33.000 34.000 34.000 32.000 34.000 34.000 31.000 31.000 Control-2 33.000 35.000 33.000 35.000 34.000 33.000 31.000 31.000 33.000 33.000 11.3 33.000 36.000 32.000 32.000 36.000 33.000 33.000 34.000 33.000 32.000 22.6 35.000 36.000 34.000 36.000 32.000 33.000 35.000 34.000 33.000 33.000 45.2 36.000 34.000 31.000 35.000 35.000 33.000 33.000 34.000 33.000 31.000 72.6 37.000 37.000 36.000 32.000 35.000 32.000 31.000 34.000 34.000 33.000 100 39.000 38.000 32.000 36.000 34.000 33.000 34.000 35.000 34.000 31.000 Intake 39.000 35.000 41.000 36.000 35.000 38.000 37.000 36.000 37.000 34.000 Transfonn: Untransformed 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control- I 33.000 0.9970 33.000 31.000 34.000 3.779 10 Control-2 33.100 1.0000 33.100 31.000 35.000 4.140 10 11.3 33.400 1.0091 33.400 32.000 36.000 4.508 10 22.6 34.100 1.0302 34.100 32.000 36.000 4.019 10 45.2 33.500 1.0121 33.500 31.000 36.000 4.925 10 72.6 34.100 1.0302 34.100 31.000 37.000 6.252 10 100 34.600 1.0453 34.600 31.000 39.000 7.235 10 Intake 36.800 1.1118 36.800 34.000 41.000 5.700 10 -4.670 1.734 1.374 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.955419481 0.868 0.544660572 0.427540628 F-Test indicates equal variances (p = 0.22) 2.3431952 6.541089535 The control means are not significantly different (p = 0.87) 0.170664037 2.100922029 Hypothesis Test (!-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.373940728 0.041508783 68.45 3.138888889 1.9E-04 1, 18 Treatments vs Control-2 sqnl01_05-12-09data
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-...1 May 12-19, 2009 0 !;~*"'!-:.
.*~~ Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses ,j Environmental Testing Solutions, Inc. Project number: 5444 Reviewed by: G vf.Aho.-7 e. ~ Concentration Control 11.3% 22.6% 45.2% 72.6% 100% 100% Intake
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Page 7 of7 Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant- Non-treated Date: OS* \'"1-cf\ Concen- Parameter tration 11.3% 22.6% 45.2% 72.6% 100% 100% Intake Page 49 of 107
Page 1 of6
!ETS
) Envtronm~nt1/ Tutln.g SaluUcna.lnc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: TVA County: Hamilton Facility: Sequoyah Nuclear Plant Treatment: UV-treated NPDES #: TN 0026450 Outfall: 101 Project#: ---='S~Y...:."\...!.~.....!-.---- Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Each concentration was treated Effluent volume (mL) 282.5 565 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2217.5 1935 1370 685 0 to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences. Test organism information: Test information: Organism age: l.o. s~ 1"\.ou.tc:. 0 '-~ Randomizing template: ~LvL Date and times organisms os-\\-c~ Incubator number and were born between:
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shelf location: 2>e, Organism source: ~ '&A\'C..H Vp O'S*\\-0'\ Artemia CHM number: t.\-\ I-\ t.\1.\ '-\ Transfer bowl information: pH= SU Temperature= oc Date I Time in: OS*!P.*o"\ \'2.00 l.~"L "1. '-\ .l.,. Date I Time out: oS*z.o*d"' l't'l.O Average transfer volume: Total drying time: "Z.'( *U 1\biJttS Q.l~CS~ Initial oven temperature: ~()*L Final oven temperature: lo\'t. Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Sample MHSW numbers used batch used 0 2 3 4 5 6 7
/
Page 50 of 107
~ETS Page 2 of6
~ EnvlronmentlfTtstln; Solutkms.lrl,,
Species: Pimephales promelas Date: OS*n-o'\ Client: TVA I Sequoyah Nuclear PlaJ}t- UV-treated Survival and Growth Data Day CUNTROL 11.3% 22.6% A B c D E F G H I J K L 0 JO /0 10 IC lO 10 /0 10 10 10 /0 10 1
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A= Pan weigh_t (mg) Tray color c~l~1- \,k Analyst: bt*v-. )3,g£" l3.bd. /3. 8i> lll.y~ Y.6o ~~-ft J'J,..bO Ill. IIf /3.g6 )It IS' 14.14 14. ~~ Date:_ o~- ld--0'\ B = Pan + Larvae weight (mg) ~0.>> 1~1. 0) j.{). tS' ~l.Olf .;l;).3S .l~. d.'l J.D.lb ~.l.~b J.1.~3 al.l.J~ ~~.~f) ;)e.g~ Analyst: ""'t... r Date: o 5 ~~o ~o~ C = Larvae weight (mg)
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~ETS Page 3 of6
) Environmentlllt:stln.g Soludor.s. tnt..
Species: Pimephales promelas Date: -~os~*-=-r-=-..;1--cD'I=--=---- Client: TVA I Sequoyah Nuclear Plant- UV-treated Survival and Growth Data Day 45.2% 72.6% 100% M N 0 p Q R s T u v w X 0 (0 l() 'D ID /0 lb 10 lfJ 10 10 10 10 1 10 fO ID 10 /0 /0 10 10 10 tD 10 10 2 IO /0 JD /Q 10 10 lb /Q IO /0 /() 10 3 10 ID /0 ID IO IO IO /() 10 IQ 10 /0 4 IO 10 /0 10 10 10 10 10 10 /0 10 10 5 to 10 10 lO /0 /0 10 10 10 If) tO IO 6 to tO IO 10 10 /D It> /'V l\) ro /0 /Q 7
/CJ 10 IC 10 10 10 10 10 ID 10 /0 tD A= Pan weight (mg)
Tray color code:: \.\\,.~~~ N.lq ll~l{ Analyst: ~l..r I~~. S"tf J~qS" r~.o" 13.4f /3. 6& 14. 35' 14.41 13.31{ 13. f>O 11{.~0 Date: 6 .c--1).-ot.\ 8 = Pan + Larvae weight (mg) ;J."J.S). J.l.ll jq,qa. ~I.Df ;).oSO .;1.1.1>3 ~.0£ ;).t>Sb Jq.q I ~t.al.( ~f. tl> J..O.yq Analyst: 1'\L..r" Date: tJ~*f.O*oa C =Larvae weight (mg)
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number of (%) 0. "'" & larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: ~ Comments: Page 52 of 107
Page 4 of6 Species: Pimephales promelas Date: Client: TVA I Sequoyah Nuclear Plant- UV-treated Survival and Growth Data Day 100% Intake y z AA BB 0 10 /0 /() IO 1
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~ Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
- ~ Environmental Testing Solutions, Inc Project aumber*
Keveiwed by: /"()/LAAt-5444 Not for Compliance Assessment Internal Laboratory QC \. ./I Cu.(Utnttioa (%) Replic.&e IaJtW **mber of Fiu.l aumber uf A= Paa weie;hl B ""' Paa + Lan-~~e Larvae ,.;gbl (m&) WciP,t I Survivia£ Meao "<lpll Coefti~t of varia1hNI Weig~t/ [aitial IUIPbu Muasurvh*af Meu weiehtt Codlicieatol Peruut reduc:tioa frota 1:1111"\'lle lalvae (m&) weigbl (mg) =A-8 umber- of larvae (me.) Sarvivlag ..mbcr of (M.alt*el&l'f>'t'MU"l'l*la' of ld.I"VK (mg) (~*) Jailial umber of varladoa tid** w.1p1 toafroJ (~*) larne(m&) .... w .rw ...~> (%) lalvae (mg) pOI-Jtl.t DUlllbu o(\arvM) A 10 10 13 85 20.22 6.37 0.637 0.637 B 10 10 13.62 2105 7.43 0.743 0.743 Control 0.684 6.8 100.0 0.684 6.8 Not applkable c 10 10 13.80 20.75 6.95 0 695 0.695 D 10 10 14.45 21.04 6.59 0 659 0.659 E 10 10 14 60 22.35 1.75 0 175 0.775 F 10 10 13 77 22.29 8.52 0.852 0.852 11.3% 0.789 5.4 100.0 0.789 5.4 -15.4 G 10 10 12.60 20 16 7 56 0 756 0.756 H 10 10 14 14 21 86 7.72 0.772 0.772 I 10 10 13.86 21 23 7.37 0 737 0.737 J 10 10 14 15 21 42 7.27 0.727 0 727 22.6'/o 0.724 18 97.5 0.706 5.3 -3.3 K 10 10 14.14 21.20 7.06 0.706 0.706 L 10 9 14.31 20 84 6.53 0.726 0.653 M 10 10 15 59 22.52 6.93 0.693 0 693 N 10 10 13.95 21.17 7.22 0 722 0.722 45.2% 0.714 4.6 100.0 0.714 4.6 *4,4 0 10 10 13.09 19.92 6.83 0.683 0.683 p 10 10 13.45 21 01 7.56 0.756 0.156 Q 10 10 13.68 20.50 6.82 0.682 0.682 R 10 10 14.35 21.03 6.68 0.668 0.668 72.6°/* 0.684 3.8 100.0 0.684 3.8 -0.1 s 10 10 14 41 21.06 6.65 0.665 0.665 T 10 10 13.34 20.56 7.22 0.722 0.722 u 10 10 13.60 19.91 6.31 0.631 0.631 v 10 10 14 40 21.04 6.64 0.664 0.698 8.5 0.664 100% 100.0 0.698 8.5 -2.1 w 10 JO 14 19 21.70 7.51 0.751 0.751 X 10 10 13 44 20.89 7.45 0.745 0.745 y 10 10 14.20 21.15 6.95 0.695 0 695 100% intake z 10 10 14 82 21 17 6.35 0.635 0.659 41 0.635 100.0 0.659 4.1 3,7 AA 10 10. 14 20 20 82 6.62 0 662 0.662 BB 10 10 12.96 19 38 6 42 Q642 .. - 0.642 Outfall101: MSD= Mmimum Signdi.cant Dtfference DunoeU's MSD vaJue: 0.0710 PMSD~ Percent Minimum Sig,nHlcant Difference PMSD: 10.5 PMSD is a measure of test prectston. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test Intake: Dunuett's MSD value: 0.0520 Lower PMSD bound determined by USEPA (LOth percentile) ~ 12% PMSD: 7.6 Upper PMSD bound determined by USEPA (90th percentile)~ 30%. Lower and upper PMSD bounds were deterrruned from the I Oth and 90th percentile, respectively, ofPMSD data from EPA's WET lnlerlaboratory VOJiabilily S!udy (USEPA, 200 l a; US EPA, 200 I b) USEPA 200la. 200lb. Fma1 Report Interlaboratory Variabthty Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-Ql-Q04 and EPA-821-B-Q1-Q05. US Environmenla1 Protection Agency, Cincinnati, OH
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Statistical Analyses j Environmental Testing Solutions,. Inc. Larval Fish Growth and Survival Test-7 Da~ Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: TVA I Sequoyah Nuclear Plant, Outfall IOJ End Date: 5119/2009 Lab!D: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% 1 2 3 4 D-Control 0.6370 0.7430 0.6950 0.6590 11.3 0.7750 0.8520 0.7560 0.7720 22.6 0.7370 0.7270 0.7060 0.6530 45.2 0.6930 0 7220 0.6830 0.7560 72.6 0.6820 0.6680 0.6650 0.7220 100 0.6310 0.6640 0.7510 0.7450 Intake 0.6950 0.6350 0.6620 0.6420 Transform: Untransformed !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6835 1.0000 0.6835 0.6370 0.7430 6.776 4 0.7361 1.0000 11.3 0.7888 1.1540 0.7888 0.7560 0.8520 5.450 4 -3.522 2.410 0.0720 0.7361 1.0000 22.6 0.7058 1.0326 0.7058 0.6530 0.7370 5.308 4 -0.745 2.410 0.0720 0.7096 0.9640 45.2 0.7135 1.0439 0.7135 0.6830 0.7560 4.598 4 -1.004 2.410 0.0720 0.7096 0.9640 72.6 06843 1.0011 0.6843 0.6650 0.7220 3.834 4 -0.025 2.410 0.0720 0.6910 0.9387 100 0.6978 1.0208 0.6978 0.6310 0.7510 8.544 4 -0.477 2.410 0.0720 0.6910 0.9387 Intake 0.6585 0.9634 0.6585 0.6350 0.6950 4.083 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.957272053 0.884 0.185406404 -0.99302071 Bartlett's Test indicates equal variances (p = 0.83) 2.111745119 15.08627224 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 > 100 1 0.072014927 0.105361999 0.0061735 0.001785833 0.023020582 5, 18 Treatments vs D-Contro1 Linear Interpolation (200 Resamples) Point % SD 95% CL(Exp) Skew
!COS 60.362 JC10 >100 IC15 >100 IC20 >100 JC25 >100 IC40 >100 IC50 >100 sqnl01_05-J 2-09data-uv
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Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample 10: TV A I Sequoyah Nuclear Plant, Outfall 101 -Intake End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EP A-821-R-02-0 13 Test Species: PP-Pimephales promelas Comments: Cone-% 1 2 3 4 D-Control 0.6370 0.7430 0.6950 0.6590 11.3 0.7750 0.8520 0.7560 0.7720 22.6 0.7370 0.7270 0.7060 0.6530 45.2 0.6930 0.7220 0.6830 0.7560 72.6 0.6820 0.6680 0.6650 0.7220 100 0.6310 0.6640 0.7510 0.7450 Intake 0.6950 0.6350 0.6620 0.6420 Transform: Untransformed !-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.6835 1.0000 0.6835 o.6370 o:7430 6.776 4 11.3 0.7888 1.1540 0.7888 0.7560 0.8520 5.450 4 22.6 0.7058 1.0326 0.7058 0.6530 0.7370 5.308 4 45.2 0.7135 1.0439 0.7135 0.6830 0.7560 4.598 4 72.6 0.6843 1.0011 0.6843 0.6650 0.7220 3.834 4 100 0.6978 1.0208 0.6978 0.6310 0.7510 8.544 4 Intake 0.6585 0.9634 0.6585 0.6350 0.6950 4.083 4 0.934 1.943 0.0520 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95557791 0.749 0.566287708 -0.42008898 F-Test indicates equal variances (p = 0.40) 2.96680498 47.46722794 Hypothesis Test(l_-tail,O.@ - * * -~--. MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.05203226 0.0761262 0.00125 0.001434 0.386520296 1,6 Treatments vs D-Contro1 sqnlOJ ~05-12-09data-uv
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iii Pimephales prome/as Chronic Whole Effluent Toxicity Test T ----- EPA-821-R-02-013, Method 1000.0 1:_:) <.o*' Daily Chemical Analyses
- --~ Environmental Testing Solutions, Inc.
Project number: 5444 Reviewed by:,L'\J~
Page 5 of6 Species: Pimephales promelas Date: OS* \"!.* 0'\ Client: TVA I Sequoyah Nuclear Plant- UV-treated 11.3% 22.6% 45.2% 72.6% 100% 100% Intake Page 58 of I 07
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I: {'--' :. EnvlfonmentalTatlng.Solvtltns.lnc. Page 6 of6 Species: Pimephales promelas Client: TVA I Sequoyah Nuclear Plant- UV-treated Parameter 11.3% 45.2% 72.6% 100% 100% Intake Page 59 of 107
Page d-. 'i Page _I_ of_l_
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~ Environmental Testing Solutions, Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Meter: Accmnet Model AR25 pH/Ion Meter Analyst Date analyzed I f'l\l.A'" (; 5 ~ JJ_ .. 0'\ Iodide reagent: 111\a Acid reagent: _ l:llR. l ~ '1-33o Calibration: I to bracket the chlorine levels of the samples. I L a b oratory controI standard: Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 number (mg!L) (mg!L) (acceptable range= 90 to 110%) lnSS 61~ 0.50 0, S'Oi> J\1"1
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Sample characteristics s LO.ooo ~U' I D LO ,0~~ i-ll. I Sample ID Sample characteristics I I I.., I I=-d Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Id L aboratory control stan dard: Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 number (mg!L) (mg/L) (acceptable range= 90 to 110%) -t '!nss t+-3 . -- o:-so D;tfttl 6\8;~~ Reviewed by ~-~~:"-:::--:-;;-----J-1 Date reviewed L--=0:-=S_:*I:...:1.::..*..;;;;o'\~___,]
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Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pWlon Meter Analyst Date analyzed I 0 S'"- ('1\.Lf" j L.j - o1 Iodide reagent: ll"A Acid reagent: _:ri\A. (2 3' 3 J 3 3 () Lab oratory controI standard: Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 I .l.t\ s~ ,,] number (mg/L) 0.50 (mg/L) o.li6lf (acceptable range= 90 to 110%) qJ.. ~~{) I Sample characteristics I I Sample ID Sample characteristics I I II Note: All samples were analyzed in excess of EPA recommended holding time (IS minutes) unless otherwise notecl
~
L aboratory controI stan dard: Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 --
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.J.,..IUJ number Ptj (mg/L)
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(mg/L) ()* l.f6q "" (acceptable range= 90 to 110%)
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'age 61 of 107
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_) Environmental Testing Solutions, Inc, r Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0. l 0 mg!L Meter: Accumet Model AR25 pfL'Ion Meter Analyst I L\40 I Iodide reagent: ""0\.) 1233 7 1: Date analyzed 0~-llP- 09 Acid reagent: ::I:Nr23X? Lba oratory controI standard: Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 I 'XN5~t.D13 number (mg/L) 0.50 (mg/L) 0.'50, (acceptable range= 90 to 110%) I OJ.~*}. I Sample characteristics s '- o.oan.1.'\ I D LO. 00011.3 I Sample ID Sample characteristics I I L().DOISlP L 0 .000\\.DCO L. o. o ao llo"1 L.O. coo003B'f I LO.OI4l1 L. o. OOS7 I I L 0 .co '1.~ I I L ab orat ory controI stan dard: Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 number (mg/l.) (mg!L) (acceptable range= 90 to 110%) I -r:_ ' ,-. Lr-_ *
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I Page
- I Page Alkalinity (Sl.\12320 B)
Matrix: Wirtet:-RL = 1.0 mg CaCO)L
~"""'fb.._i5"-L----~ I \15Ll*
I Analyst Date analyzed 05 -I D *oar Titrate samples to pH= 4.50 S.U. Time initiated Time completed ==l==fi::5======== I Titrant norma 'tty and multiplier determination: pH of Deionized Titrant Normality check Begin End. Total Normality (N) of H 2S04
= (5 ml Na 2 C03 x 0.05)/E pH Factor or Multiplier
= (Nx 50000)1 100 ml sample water reference standard ml ml ml =0.25/E =Nx500 I =4.5 t.t,5 s.u. number Tt-li2-62..CJ number JN%(.;.'5.~ t).O 1/."1-(E) 11.1 (acceptable range= 0.0180- 0.0220) 0.0-Rl-Y. 10.":1-I Lb a oratory controI stan dard:
Reference standard number True value (TV) Sample Alkalinity (MV) % RS = MV I TV x 100 (acceptable range volume Begin End Total Multiplier (mg CaCOJIL)
= 90 to 110%)
I (mg CaC0 3/L) (ml) ml ml ml SNSS ~54 100 100 11.--::r- ~C.(p 'eq 10.1- cvs qs"i.c I Multiplier Alkalinity (mg CaCO:YL) o/oRPD= {(S- D) /((S+D)/2)} x 100 (acceptable range=+/- 10%) I s D I I M.' atnx spJJke recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) I i INS.SvS4 (me CaCO:YL) so (ml)_ laO ml
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ml o-t-o ml 10,"1- ID.1- l\0 I Sample alkalinity (B) (mg CaCOJIL) Measured spike value (MV) MV=A-B (mg CaCO:YL) Ofo.R= MV I SV x 100 (acceptable range
= 75 to 125%)
I samp1el measurements: lo3 4-::t- C\i-~ Sample volume Begin End Total Alkalinity I Sample number OS- lYJ.ty::, B rn tJ~ Sample ID 1JtiJ 1')\) (ml) ml 31-.tl ~-~ 5.1-ml ml Multiplier l () .t-(mg CaCOJIL) vl fx5. () t)-0C1 (!; J 4J..1- lti_\?'t ~*1- \ lol I b:s- 6-e.oCJ 6Sv.J 'D.O 2_,q ~.1 ir.t'ii'7. ~~
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Page ~ of lo 1 Alkalinity (S~fl320 B) I
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Analyst l Date analyzed.~~"~
- c I Matrix: Water, RL = 1.0 mg CaCOy'L Titrate samples to Time initiated ,...............
Time completed t---__;:::o.....::"'4(;~,__--l
~5-ID*D9 pH= 4.50 S.U.
Titrant normality and multiplier determination: pn 01 N"P"1!0ijty pH Factor or Multiplier Deionized Titrant check JSegm . ...Tntll) Normality (N) of H 2S04 (5 ml Na1C03 x 0.05)/E (Nx 50000)/ 100 ml sample water reference standard ml ml ml l\ 'l.<;/F. Nx500
-4.5 s.u. number number (E) (acceptable range 0.0180 - O.u-.-.u, I.
I L aboratory control standard: Reference standard number True value (TV) Sample volume Begin End Total Multiplier Alkalinity (MV) (mg CaCO:Yl)
% RS= MV /TVx 100 (acceptable range (mg CaCO:YL) {ml) ml ml ml = 90 to 110%)
' :r t-)SS fo 54- 100 100 a-t.o .lf3:t CiLJ- 10.1- H.m-~~~ .i'n 7VV~j\l)
~
i' Alkalinity %RPD= Multiplier (mg CaCO.YL) ((S- D) /[(S+D)/2]} x 100 (acceptable range= ::1: 10%) s l:] D I M,. atrvc sp1'ke recovery: Reference standard Spike value Sample Spike alkalinity (A)
*I number (SV) volume Begin End Total Multiplier (mgCaC(}y'L)
{mg CaCO:YL) (ml) ml ml ml
]:NSSt..u5Y* 5o 100 tO.-=t- :l,t.?.D 15-3 ID.-: !(.QO Sample alkalinity (B) Measured spike value (MV) %R =MV I SV x 100 i (mg CaC03/L) MV=A-B (acceptable range
= 75 to 125%)
I (mg CaCOJIL)
+re-~ 110 ='() fo~01o sample l measurements:
I Sample number OG- DC\ *0'1 5A-u~uJ Sample ID Sample volume Inn (ml) Begin ml
;/l.>.C End 35.5 ml Total ml q.s Multiplier
\i);-:f-Alkalinity
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~ T" Page Page 3 of_lo=-- } Environmental Testing Sol~~;:l.*;nc, Alkalinity (SM.-2320 B)
Matrix: Water,- RL = 1.0 mg CaC03/L I "'-- Analyst t-l-""6'-=St.:""-:;.._--:----IJ Date analyzed [ 05 -I 0 -O.:J' l Titrate samples to Time initiated Time completed ..__ ______ t--~-:::::.......
-=::;:t::---~~\:;;--l
_~ pH= 4.50 S.U. Titrant normality and multiplier determination: pH of Normality (N) ofH1S04 pH Factor or Multiplier Deionized water Titrant reference check standard Begin ml Enu ml J.' ml
-* 'f5 ml Na1 C03 x 0.05)/E 0.1;::1/J!.
- (N x 50000)/100 ml sample Nu:_ 500 4.5S.U. number number (E) (acceptable range 0.0180 - 0.0220) -1'-
L ab oratory controI standard: Reference standard True value Sample Alkalinity (MV) % RS :MY /TYx 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaCOJIL) (ml) ml ml ml =90 to 110%) J: N ss lo5'-l- 100 100 D.D C):'f <14 io:1- 1010lo ID\ Alkalinity %RPD= Total Multiplier (mg CaCOJIL) {(S- D) /((S+D)/21} x 100 (acceptable range=+/- 10%) 1D-:t- ~ \ lO atru: spz.ke recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SY) volume Begin End Total Multiplier (mg CaCOJIL) (mg CaCOJIL) (ml) ..mJ.,..... ml ml IY~~~54 '2JCe~ ~\)0 . ~~- . .... re~~~ .'1 ~ lb.i- ~~_..310 Sample alkalinity (B) Measured spike value (MY) % R: MY I SY x 100 (mg CaCOJIL) MY=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) 110 O?oD l'uou1-o measurements: Date reviewed: I05 _\ o-09 J
Page ~~,!~~,,
-to Page Lf of Alkalinity (Sl\1!~320 B)
Matrix: Wme~RL = 1.0 mg CaC03/L Analyst Date analyzed It-c. .~L
)~ . 5-~===1-D---D-4--f Time initiated ~ I Time completed 1---.:::::::..----jb=db---l Titrate samples to *I pH= 4.50 S.U.
r:ztrant normaItty an d multiplier determination: pl!_of Normality Normality (N) of HlS0 4 pH Factor or Multiplier i Deionized tl~l'anl .-h ...-k Begin End Total =(5 ml Na1C03 x 0.05)/E = (N x 50000)/100 ml sample water reference standard mr- o.l ml 0.251E Nx500 4.5 s.u. number number (E) lacce .. ~ = n R180 - 0.0220)
.....1 I L ab oratory contro1stan dard:
Reference standard number True value (TV) Sample volume Begin Multiplier Alkalinity (MV) (mg CaCO:v'L)
% RS = MV I TV x 100 (acceptable range End Total I X'"NS'S(oC:S~
(mg CaCOJ/'L) 100 (ml) 100 ml ml 11-f-.?...- l~s-ml q_3 ) D.-:} 100 100~
=90 to 110%)
I Multiplier Alkalinity (mg CaCO:v'L)
%RPD=
{(S- D) /[(S+D)/2]} x 100 (acceptable range=+/- 10%) II I uatru:
- spt'ke recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (mg CaCO:v'L) I (SV) volume Begin End Total Multiplier (mg CaC03/L) (ml) ml ml ml r"Nss <.co4- .Qa'D c1S ~5-~ 32.'73 1-D L4) IIJ.t- 3DD I Sample alkalinity (B) (mg CaCOJIL) Measured spike value (MV) MV=A-B
% R = MV I SV x 100 (acceptable range
= 75 to 125%)
(mf CaCOJIL) I Samp*I e measurements:
-HB~ \CO <X co ~~
Sample volume Begin End Total Alkalinity I Sample number d\OS<>~*o& -rMBtF SampleiD 3 as (ml) ml
~2.?., 35-3 ml
~5 ml L4)
Multiplier
\D.-1--
(mg CaC03/L)
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Page 3\p Page 0 of~lp""'--- Alkalinity (Sl\1:~320 B) Matrix: Water;-R.L = 1.0 mg CaCOJL Analyst I~ Date analyzed D~D *Q'j Time initiated 11---2'!~.,...._,_ _ _ _---i Time completed ~ Titrate samples to pH= 4.50 S.U. I Titrant normality and multiplier determination: Jrn-c-Deionized water Titrant reference Normality cue"~ standard _R .. mn ml End ml Total Normality (N) ofH2S04
- (5 ml Na 2C0 3 x 0.05)/E pH Factor or Multiplier (Nx 50000)/ 100 ml sample Nx500
(~;
"-25/E I 4.5S.U. number number (acceptable range 0.0180- u.w;.w, i I L aboratolJl controI standard:
Reference standard number True value (TV) Sample volume Begin End Total Multiplier Alkalinity (MV) (mg CaC()JIL)
% RS=MV /TV X 100 (acceptable range I tc NS.S L:> nti-(mg CaCO)!L) 100 (m!l 100 ml rr.s~
ml 1:}-:{., ml ct:z... l0 q~
=90 to 110%)
q~~ I End Multiplier Alkalinity (mg CaCOJIL)
%RPD=
{(S- D) /[(S+D)/2]} x 100 (acceptable range=+/- 10%) I I atriX spz"k e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) I I.I~5Stn5+ (mgCaCO)!L) IDO (ml) 5D ....,~ ml
....,~~~
7~ ml
$)5 1 ml
-Ho l?) 10:1- G)g I Sample alkalinity (B)
(mg CaC03/L) Measured spike value (MV) MV=A-B
% R = MV I SV x 100 (acceptable range
= 75 to 125%)
(mg CaC03/L) I S amp*eI measurements: 53 40 Joo0"1b Sample volume Begin End Total Alkalinity I Sample number o~oSo'-l~oS 1 Sample ID
-wri VJBn I t-z_ J (ml) 60 ml 3o.S 261 1 ml ml
.LJ..(Q l2.
Multiplier 1o.--=t-(mg CaC03/L) q~ o'\oSo~.o.u I 2. .~.I 34.$ 4.'-t_Lt '1Ll-I ol\ us.o&-. *'"L.. J 3 ?,9S J..f?,.?, ~g b
~~
<6\
0<1n41;.r..4.0"=>> 1..fA vuBn Jl2tn1 1 1.!-\-;_:s .!--\!+-~ \.0[7... I 1'"\" 0'50\o * 'tl O~O~C>i, \~ J I 3 2 ~t.3 [L\-S_ ~ 40.'2-
~--b Lt> t1.
D .9_ L7 c?\ ICl 1- 0~ bSOt.l --:.w41A)0f\} uv ) lfu'W j)--- - l~'f* rw.--9D-9 J4.-::t_a IDI
~_m tiC:.
r------- 0 _q
-~
1"'1~0~0\..zR 2 5-~ "+!+ L2 <1t.t 0'\~-SOf* \1... J ' 5.3 q.\ 3.~ l"Z~ ...........- Sl I age 67 of 107 Reviewed by:
i
~ *
*e 1(1)
Page lq Page of__,lo'---
~
~. i
. } Environmental Tming Solutions. Inc.
II Alkalinity (SM:,2320 B) Matrix: Waier;-Ri = 1.0 mg CaC03/L
*I Time initiated I'::::-=---,__
Titrate samples to Time completed 1----'=------Pc=-flr-----i pH= 4.50 S.U. Titrant normality and multiplier determination: t'~A
.. Nnrmalitv Normality (N) of H 2 S0 4 pH Factor or Multiplier Deionized Titrant check Begin .l!.na 1V'401 ft::. ml Na,C03 X 0.05)/E (Nx 50000)/100 ml sample water reference standard ml ml ml 0.25/.1!. A.-Nx 500 I -4.5 s.u. number number (E) (acceptable range = 0.0180 - 0.0220)
I L aboratory controI stan dard: Reference standard number True value (TV) Sample volume Begin End Total Multiplier Alkalinity (MV) (mg CaCO;,/L)
% RS = MV I TV x 100 (acceptable range (mg CaCO;,IL) = 90 to 110%)
I (ml) ml ml ml 100 too~ lJN'SS \oV)L-} 100 q,' ~~ it cv3 lb:-1' 1-oo I Sample ID Total Multiplier Alkalinity (mg CaCO;,/L)
%RPD=
{(S- D) /[(S+D)/2)} x 100 (acceptable range=+/- 10%) I lc.1 s D
~o.o - M.. atrtx SPI.ke recovery:
Reference standard number Spike value (SV) Sample volume Begin End Total Multiplier Spike alkalinity (A) (mg CaCO;,JL)
'I (mg CaCO;,/L) (mil_ ml ml ml S NS.<:) '-o5 '+ IOO 50 c5/1).0 ,;(tQ .o l.I>.u b.\ ID.1- 130 Sample alkalinity (B) Measured spike value (MV) % R=MV I SVx 100 (mg CaCO;,IL) MV=A-B (acceptable range (mg CaC031L) =75 to 125%)
q(.p 2>4 qltcrw s,amp*Ie measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO:YL) 01\ 0$0(... 'l.'i. -r:JA l/JBf) 'I] INW l a) ~~.0 ld-1-.0 ro l-t lb.1- d\ o tto~o.f. l'!l j 2 so ~':f.\) L:J'i?. '2.. .1*2.. Lz. .:<tt.
' oc:; e, s CT'l. o-s 0'\0SCJ1* 0'...:.
£12m- 00!
eJZ.lfn \JD l)_Q l 50
~100 a~ (3~.
~8.1.. .~o:'+ I..?SLL)
.30.1-
......... ~ 4.~.5
'.~~.4 1-~
54-81-
' o4\oscn .en E:F-m {)02_ 5.\ Ltt) Ol0\0
.tio~\) ,q:~ _v 0'\0Scrl* 0~ &.ll-YY'- uoW 2- 100 - ,::l, 10. \p 110
- - f------* --
~-*
I.,. os-01*o16 IY\ HS \fV r::::; JDO ~-3-~ ,;}'.;_g. 59 --
- .. - -="""
'age 68 of 107 Reviewed by: Date reviewed: I05 -10 oCJ
Page -5t) Page :1 of 3
--=---
~) Environmental Testing SoluUons, Jnc.
I Analyst I &:,!. Alkalinity (SM2320 B) Matrix: Water, RL = 1.0 mg CaC0 3/L Time initiated 1~~ SI I Date analyzed 1-o-c.""':::;""'-,""';'--.D-j-,----1 Titrate samples to pH = 4.50 S. U. Time completed ~~~3....,5=..:.....ol'-------1
'---=-"-----___)
I Titrant normality and multiplier determination: pH of Normality Normality (N) of HzS04 pH Factor or Multiplier Deionized Titrant check Begin End Total == (5 ml Na 2C0 3 x 0.05)/E == (N x 50000)/ 100 ml sample water reference standard ml ml ml = 0.25/E ==Nx 500 I == 4.5 s.u. 5.0 number rNe,32.9 number J'NSS\p5'3 t:.l 1\.lc (E) II.'() (acceptable range= 0.0180- 0.0220) o.o~ r+ ID.9 B\ k:. ~rr.u.nffi'< o.o -=J c *I I L ab oratory con tro I stan dard: Reference standard number True value (TV) Sample volume Begin End Total Multiplier Alkalinity (MV) (mg CaC03/L)
% RS = MV I TV x 100 (acceptable range (mg CaCOJIL) = 90 to 110%)
I (ml) ml ml ml S NS"S \D 5!...1- 100 100 '6.1-- I D-9 <:15 <-ts~Yio II lo "'1)3 I Sample ID Sample volume End Total Multiplier Alkalinity (mg CaCO:IL)
%RPD=
((S- D) /((S+D)/2]} x 100 (acceptable range=+/- 10%) I J mtts tho Duplicate (B) _L 0.0 'I M,atrrx ' sp1'ke recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaCOJIL) (ml) ml ml ml
- r-ws~ I.e 54 50 1\:;t:: ~'5 '6 ,3(.?.0 \0.'2.. /C'.C! }10 I Sample alkalinity (B)
(mg CaC03/L) Measured spike value (MV) MV=A-B
% R = MV I SV x 100 (acceptable range
= 75 to 125%)
(mg CaCOJIL) I SampJe I measurements: too .-i:rt).&;.c 50 !Oo01o Sample volume Begin End Total Alkalinity I Sample number 05'13-CP.I mtts t-11-0 Sample ID IOO (ml)
.~.0 ml ml
-41.5 5.S ml Multiplier IC:..q (mg CaCOy'L)
((I(\ ()S. ~ltl-Oc.1 -l I 4lS ,J.i1, l 5.\o G?l I o~-0'\.- eftA (r)\1 .s. uv I r..L2.~c 5.lo '5\.p G1
& o 5'+ !54 -I OS*Cfll*d\ z. 6.~ II 0'5 - n. -o~ j 3 ll 0 l!R 5 r::::.r-:1 (aO 05~1 Z-c;P) SaQJSW I \t r::.::; ~If .i q ~p 100 os-~~-0"') L -- - -d"W-:1 .. 35'}- L'~ -- -H-<:J-- ------~- -
r~e< .r.:-o \n ih.Aac::...n ., n n I.'J ')C rl. '0 '\ "\ .,
- >\
rt
---~
~
-"'- ':JI""
o't b~tz..o \ ~--L w 0<;d ( .2-... 3S.! !-J.lu- 3.5 *- 3g
'age 69 of I 07 Reviewed by: thO-(I Date reviewed: ~- f-(§' - I
Page --"3"-9-L-_ Page cQ of---=3"----
!) Environmental Testfng 5olutlon.s, Inc.
Alkalinity (SM23:20 B) Matrix: Water, RL = 1.0 mg CaC03/L Analyst ~C- I Date analyzed !-0-=-5-.-r-+*-O-~-.- - i Time initiated I~ I Titrate samples to Time completed ~ . pH= 4.50 S.U. Titrant normality and multiplier determination: pH or -"' ality Normality (N) ofHzS04 pH Factor or Multiplier Deionized Titrant check e.,... ..E.tuL Total = (5 ml Na2 C03 x 0.05)/E = (Nx 50000)/100 ml sample water reference standard ml ml ml - 0.25/E -Nx 500 4.5S.U. number number (E) (acceptable range O.Ul!!U- v.
~
L aboratory controI standard: Reference standard True value Sample Alkalinity (MV) % RS= MY /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO;VL) (acceptable range (mg CaCOJIL) (ml) ml ml ml = 90 to 110%)
- r1-JSS\.Q 54- 100 100 b.O CiH q~ ID.q 102... \D"2 C7.p Alkalinity %RPD=
Multiplier (mg CaCO:VL) {(S- D) /[(S+D)/2]} x 100 (acceptable range=+/- 10%) s t'O*CJ D J_ M atnx spt"k e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaCOJfL) (ml) ml ml ml
- r~c.a5~* Fj() 100 14-.'2... ):3.1 8.9 jD.q ~---C)!'}
Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaC03fL) MV=A-B (acceptable range (ml! CaC03fL) =75 to 125%) 5d-- ~~<; ~ C1n.o*l measurements: Date reviewed: I 05 -1 't D I _]
Page LtD Page
-of 3
-~ Environrf'utntal Testing Solution$, Inc.
Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCOiL Analyst~ ~L Date analyzed r[""""'os~-- .. -tt----Q~-,--1 Time initiated ~ I Time completed 1---_..::::......._~::-----l Titrate samples to pH= 4.50 S.U. Titrant normality and multiplier determination: D':
,u .~
water
. d eiOmze Titrant reference
- ~ormality checK standard
~
~ '-mt End Total
.I Normality (N) of H 2S04
= (5 ml Na 2 C03 x 0.05)/E
= 0.25/E pH Factor or Multiplier
= (N x 50000)/ 100 ml sample
=Nx500
=4.5S.U. number number (E) ~n2e = 0.0180- 0.0220) 1---- 0.<1 L aboratory control standard:
Reference standard True value Sample Alkalinity (MY) % RS = MY I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJII,) (acceptable range (mg CaC03/L) (ml) ml ml ml = 90 to 110%) 100 100 100~ INSSlos4 D.D '1.2. q.'l lv.q )oo Sample Alkalinity %RPD= volume Begin End Total Multiplier (mg CaCOJII,) ((S- D) /({S+D)/2]} x 100 ml ml ml (acceptable range=+/- 10%) 3 ~-'2.-
-1 11.'1-atnx sp1 e recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier {mg CaC03/L) (mg CaCOJIL) (ml) ml ml ml
- r,~.;s 054 iDO 50 11.1- I 't::t 1- .D b lt . q ) 50 Sample alkalinity (B) Measured spike value (MY) %R=MV/SVxiOO (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%)
$1-' q~ cto"W measurements:
End Total 71 of 107 Reviewed by: Date reviewed: J <ill/ 11 Oj
w I Page j_ Page of....cl!J=:-._ I Total Hardness (SM 2340 C) I Analyst I05f>>SL-10.~- RL = l.Orog CaC03/L _-_ --*-,r"
~:-:* Fo Time initiated IOc:fo<t-I Date analyzed bC)
Time completed ~==I~\=3~2=======~ Titrant normality and multiplier determination: I Titrant reference number Normality check standard number Begin ml End ml Total ml (E) Normality (N) of EDT A
=0.2/E (acceptable range= 0.0180- 0.0220) pH Factor or Multiplier
= (N x 50000)/ 50 ml sample
=Nx 1000 lrNRcoz. J:I\JS551-I D.-D J{).O to_o o.v2.0 ~.D I L aboratory controI stan ard:
Reference standard True value Sample Hardness (MV) % RS=MV /TVx 100 I number (TV) (mg CaCOJIL) volume (ml) Begin ml End ml Total ml Multiplier (mg CaCOJIL) (acceptable range
=90 to 110%)
[Il-JSS(Q5{p 40 50 /D.O 12.0 ~-0 2.D.O Hc.'D IDoVto I Hardness %RPD= I Multiplier 2'U .o s (mg CaCOJil,) {(S
- D) /[(S+D)/2]} x 100 I
D atri.x soike recovery: I Reference standard number Spike value (SV) (ml! CaCQJIL) Sample volume (ml) Begin ml End ml Total ml Multiplier Spike hardness (A) (mg CaCQJIL)
!INS'S> Lo~~ 5o G;,_q 21)_ \)
I l-10 Sample hardness (B)
!Ldp ~3.5" Measured spike value (MV) tJ..i-0
% R = MV I SV x 100 (mg CaCOJil,) (acceptable range I
MV=A-B (mg CaCOJIL) =75 to 125%) Cp--}. .,t.{tp I 1501.o s,amp*Ie measurements: I Sample number SampleiD Sample volume (ml) Begin ml End ml Total ml Multiplier Hardness (mg CaCQJiL) Blank TV=ND PO I OS.oq -~ (should be= 0 rng CaCO:VL) f))MS ~}2.0 too ;(3 l.x&.~
.s ~3.5 idlb.l --=liL
{).0 20.0 q7_ 05.D". *OC>{b ..1 1.28.1 ~-~ 14-.Y- '2g I f):J{u~-D J/l~<<: }"\ I ..., c q J/c SSv.J ia5 l~'+.t:1- ,)_;).. .Y.4
~ J'
' I~ ,'5-J_7 [:+/-. :::> 'r\)
I I n1-o5-IY1 R 6'5 *05.0'1 t6 m li c:... t-lz)) f-
?,q '1 IL8.'l t-J.. f}
Ji6,q J...ftl!+ L4-.IJ
~4-ao
-1 CP-u -ro~r1
.N~ ~-(;I OJ)
If' 4<1:" !-~ qq: -~ ----
~- -~ ~-=tl.t '-\Z..
l 0'\~* 0 "\ 6i:;;rl.n:f'J'*-I* 1 wa~h*Nv,~
+ ~.$'-lj
~
~
, ::y.
u
~
- l k_B;6.0
~~I 5.\3 1\.J
_j,_ 12\l
*0>{7 u
** abe 0 '-'
t :If >15m! of titrant is used, sample must be diluted.
)\...l. wei. . Reviewed by: I 1'\Ci;v J Date reviewed r {)6 -10.0'1
~--
1 Page ~ Page lftJ ofl.o..l:::___ I Total Hardness (SM 2340 C) I RL = LO m,g CaC0:/1
-- _..:.*,r
~:-::- Time initiate~
Time completed r--1--....;::::--..,~,...____,.---1 I Titrant normality and multiplier determination: Titrant I Normality check Begin End Total Normality (N) ofEDTA pH Factor or Multiplier reference standard ml ml =0.2/E (N x 50000)/ 50 ml sample number number (E) \3C 0.0180- 0.0220) Nx 1000 lA I L ab oratory control stan dard: Reference standard True value Sample Hardness (MV) % RS = MV J TV x 100 I number (TV) (mgCaCOYL) 40 volume (ml) 50 Begin ml End ml Total ml Multiplier (mg CaCO:VL) (acceptable range
=90 to 110%)
rrN<-._;::, lQs- v, if~ 105~
~-'
;?3.0 6}5. \ 2.0.0 I Sample Hardness %RPD=
volume {(S- D) /((S+D)/2)} x 100 I End Total Multiplier (mgCaCOYL) i \).0 s I Duplicate (B) D atru; spike recovery: I Reference standard number Spike value (SV) (me: CaCQ:VL) Sample volume (ml) Begin ml End ml Total ml Multiplier Spike hardness (A) (mg CaC03/L) Il-J'SS to 5{_p J.+o 5o ~B~- :t,.'} 6.3 I 21::1,0 110 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOYL) MV=A-B (acceptable range I I leLJ? (mgCaCOYL) J-.t'-t
=75 to 125%)
lto\>Lc samp,l e measurements: I Samole number Sample ID Sample volume (ml) Begin ml End ml Total ml Mult~lier Hardness (mgCaCOYL) T1L~Nn Blank I o"' o~ en. (), A-Pw
,., u1a be 0 mg CaCOJIL)
- z. ~~<SG 5o 33.1- ~to.IQ 3.\ loD 02-c~ C1'5~, . oL- J 3 31,--~ P1'l-'f' z_q f-)'8 I o~os&i. D, SoUh~ I Yr.*+ H~H- ~."+ 5li-D~ O~lJl. IQ I 'z '-1J4 m_q c<.S ! SD I 0'\ 0 !:lOt; . 0~ J 3 .J-1-tf,~ H-hl _d/5 SD qo
. C'\ 4* "'2.\* () q CJ"\
'IY\HS
----~-
V\J I 0.0 IJ.-fS. ~ y_*z, ~~ t--- q4 1 C'--\* 'l.~* ()S- oS-0'\ A I 2- l.t-+.S q,,..L-I ,;J 13."} 14.CS ~!O o5*(fl-:-~~ f.\ J c.t ----- ~3-f- f'6-1.. !-+S ~ Cib Date reviewed I OS ~0 .Oj
'I>';'f
.3 Page _91__
i Page of_Lo=-- Total Hardness (SM 2340 C) I Analyst I tbsc RL = l.O:!!Jg CaC03/L
*~:-:*
' Time initiated , .............
Date analyzed 05 ~ \0. OCf Time completed 1--..;;::::o...,~.,..------1 I Ti'itrant normality and multiplier determination:
.-h .... k I
Titrant l'llll Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard m1 -ml ml 0.2/E (Nx 50000)/ 50 ml sample number number (]!;) ~~table range= 0.0180
- 0.0220) = Nx 1000 I Lba oratory contraI stan dard:
_Kv Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 I number (TV) (mg CaCO:YL) volume (ml) 50 Begin ml End ml Total ml Multiplier (mg CaCOJIL) (acceptable range
=90 to 110%)
t11J:;s IQ.=5lP 40 t-.2. ~0.2- ~:o 20.0 4u lODt>zc. I Hardness %RPD= End Multiplier (mg CaCOJIL) {(S- D) /[(S+D)/2]} X 100 I I a rtx spt e recovery: I Reference standard number Spike value (SV) l_mg CaC03/L) Sample volume (ml) Begin
~ ml End Total ml Multiplier Spike hardness (A)
(mg CaCO)!L) f>O )o_.n. ..s:J. lrtJss c..os Ll' ~5 ~ 35~ lR-'2.. (2 I) 2o.u ;<5o I Sample hardness (B) Measured spike value (MV) % R=MV /SVx 100 (mg CaCO:YL) MV=A-B (acceptable range I ~lo (mg CaCO:YL) B()
= 75 to 125%)
I O~:>G"Lo s,ampJe I measurements: I Sample number SampleiD Sample volume (ml) Begin ml End ml Total ml Multiplier Hardness (me CaCO:YL) Blank i. TV ND I dl\OSOI* 0'1-s ould be (ljj(l{L & 0 mg CaC~IL) t.. .P8 3.5-Jf ~Q:2- 1~:?> Czi 2-D.O ) ID ld'lc':>>o'\. ot.o 4 3 BS :2,9-,,?., ~2.(o l-4~* 0- n~o I d1o'SOS. c\ Cha1i IUI!lt) t:;Oi. v I JS_ 4-1.l.? '4fl.-:l. 1~.3\.Z l3o opt os<r1. o \ I 1-. as J-{5 Cj "-1-4.<3 3C1 (J.\ lh~ C~O'So~ o~ I ..3 a.:; t).t> ;;,-::} 3.1" (~ \5{) 011 oS ()'-\ . Cl \ 7/A 6Rr I JIS 3 ,':f- ~-~ 4.'9 o,; 1Gt0 *.,. IOfl..,
-~- --* -*
cYtoSOI.>.l"( ---z:.. ts.S 1?-.J.t 1-f.q ('i ~co ~ lqv-o"'OSOi* Of _J \.~!-+ i iK ,\ .Y...:} IJ- lctO ~ \0'(') ()'\ OSO"\. 01.._ (.J A P->R~ lnf f <>J L'tS. i ~J.CO 31- b./) -L-15D -:- Il.\ -6 lage 74 of 107 . . NOte: If >ISm I of titrant ts used. sample must be diluted. Reviewed by: Date reviewed [ 0 "S . i(). (fl
Page 1{ Page i-f of b Total Hardness (SM 2340 C) RL = 1.0 ~ CaC03/L Analyst I =5"-l.t)*Qq
~SL - * ~.::--- Time initiated Time completed I1-__::""'::::;:o:::--.
--~---1 Date analyzed Titrant normality and multiplier determination:
Titr.ant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference stanuaru ml ml =0.2/E = (N x 50000)/ 50 ml sample number number lEi- ~--(acceptable range= 0.0180- 0.0220) =Nx 1000 I 1 I L aboratory controJstan dard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 I ' number (TV) (mgCaCO;VL) volume (ml) 50 Begin ml End ml Total ml Multiplier (mg CaCOJII,) (acceptable range
= 90 to 110%)
~
'TlJ;:S tonto 40 1.2 \.'6 kH.ta ~r'O 2.'U. '() J-.J.D.(J fOOO"'Zp Hardness %RPD=
I Total Multiplier (mg CaCOJII,) {(S- D) /[(S+D)/2]} x 100
)
I : atrrx spz'ke recovery: I ! Reference standard number Spike value (SV) (me CaCOJIL) Sample volume (ml) Begin ml End ml Total ml Multiplier Spike hardness (A) (mg CaCOJIL)
..:t:NSS~5!P BD ~6 ~*1- 51+ cz. 2{).() ..Qo1o I Sample hardness (B)
~1-:3 Measured spike value (MY) % R = MV I SV x 100 (mg C.aCO;VL) MV=A-B (acceptable range I \l-tD (mgCaCO;VL) = 75 to 125%)
Sample measurements: ' Sample number TV *@_ ol\ ()So g. o~ Blank o'\ Cl !:.o'-1 . o~ I{Avv&N Sample ID u<::
- u mg l
-1JA &-f: I f'lt 3 aC03/L}
Sample volume (ml) Begin ml End rnl Total ml Multiplier Hardness (mgCaCO;VL) I '+o 101 I o'\ oso"".-z.1 I 2. I - - i-- Lage . , 75 of107If
]\!ote: >I Sml o f.titrant ts
. used, sample must be dtluted.
. Revtewe
. dby: "v
)lt.
11 / L.--'--'--"----__j Date reviewed I 0 6 -\0 1fJ ]
Page '-f<;"
'Ill
'li'1
- (i}
Page 5 of Co
~:)E-.VI-.ro-nm-en-ta-1Ttiti
___ ng-So-lut-lon-s.l-nc. Total Hardness {SM 2340 C) RL = 1.0-mg CaC03/L
~ ?7 Time initiated I':. . . .,
Time completed t----'"""-<=:::::;:~---~--1 Titrant normality an d multiplier determination: n Titrant ~ormau~y ***~~- End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ............. 0.2/E - (N x 50000)1 50 ml sample number number <ii) (acceptame
- 1\ llll!O- 0.0220) Nx 1000 1
T L aboratory controI stand,ard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJiL) (ml) ml ml ml =90 to 110%) 40 50 ~5.S
- TtJSS \oSI., 1:3.5 ;)..O 2o.D L-lo £Do0"-u Hardness %RPD=
End Total Multiplier (mg CaCO.,IL) {(S- D) /((S+D)/2)} x 100 M,atrlx spz'ke recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (mg CaCO.,IL) (ml) ml ml ml
.'Jr-. f;t::t trN3""(o Sl.P ~\) l:> . -~ .~l.q -4.~ l'z Z..D.u l'lD Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCO.,IL) MV=A-B (acceptable range (mg CaC(h!L) =75 to 125%)
C\;l.. 1ro q~Olo i I sampjel measurements: I Sample volume Begin End Total Hardness
,, J Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO.,IL)
Blank TV '.@ II _v1115 L..aL..VJ/ LJ I CA o-5 0'-1. ot.. ""Qit wBfliiZ..If)i I as ~r.q ~~-~ D.9Cz D -~-2-t).O ?,-(, 0'\0SO'-*~ z ...:U.'6 .J3.(p 0.~ '.2~ 32..
*~
L Io~ osul. 1.3 J .3 ~3.to &4.1. D.\.+> (z c;!Y 0 '\OS 0 '-\
- 0" r J.~r .u RIU 11z.m e<£.1 lc?Li-.~ lcn..o 1~9. u 110 -;.112-n~CJS0'-*1.~ I 2 ~-:J-.0 ~q.~ ~~-~ t'b \00 -: 10 '1 a'\ osocf. 12... j .3 /)O.J.o I~Lq c:l.~ r., 92.
- r----- n'\OSCI~.Oio liA IAJ-3iJI lt.-1rrl;OII I 31q 1~2.~ ~Pi tz_
.~~
i oqosol.* 2.t:t l 2 I3d..~ 133.5 o:--:ru. ,;('f.
~ _!.-
o'\oSoil* I~ J 3 33.'5 ~.)_ b:Ht ~~ Date reviewed I OS *\0 .(.:11
I **
~
Page 1'f::> tl
~
Page Le of_,_L,~- I -~ ~~vironmentaiTestlng Solutions. Inc. Total Hardness (SM 2340 C) I Analyst IB ---- RL :=' LO~g CaCOiL
~:-:::-
Time initiated It--""'-SJ;;o:::::-----i Date analyzed l)]ft 1tHJ<='\ Time completed I Titrn ..t - .JJ. t* and multiplier determination: I Titrant reference number Normality. check standard number Begin ml
.t;nu ml A
ml (E) Normality (N) of EDTA
-,U *..:d~
(acceptable range- 0.0180 - 0.0220) pH Factor or Multiplier (N x 50~ 50 ml sample
= X 1000 I L aboratory controI stan ard:
Reference standard True value Sample Hardness (MV) % RS=MV /TV X 100 I number (TV) (mg CaCOJIL) 40 volume (ml) 50 Begin rnl End ml Total ml Multiplier (mg CaCOJfL) (acceptable range
=90 to 110%)
II:NSS (pr;)lf 2s-+:1_ Ph2 ~*.o 2.D_O J-ia too07....o I Hardness %RPD= I Multiplier (mg CaCOJIL) {(S- D) /[(S+D)/2)} X 100 2{).0 s H5o D I M.atriX . sp1"ke recovery: I Reference standard number Spike value (SV) _(mg CaCOJil,) Sample volume (ml) Begin ml End ml Total ml Multiplier Spike hardness (A) (mg CaCOJfL) ITNSSG>5~ 3D 3q,q -454 5. St:z D2-tl.U O?ao I ~5 Sample hardness (B) Measured spike value (MV) % R = MV I SV x I 00 (mg CaCOJil,) MV=A-B (acceptable range I l50 (mg CaCOJIL) 1D
=75 to 125%)
~'Dqo sam_pJe l measurements:
I Sample number Sample ID Sample volume (ml) Begin ml End ml Total ml Multiplier Hardness (m2CaCOyL) TV= Blank II. I o~ OS 11'\.0I. (should be= 0 mg CaCO:JLl lelUn vo 001 50 )~;:\_1...} 5D.D lf.lo )0.0 qL. ol\oscn.a-t ~ Oaz.. /0 fil I~IO.C) qq l5) otqo I o'\oscn. o~ ElZ.t'n 00002- .50 J_ J.B.o 33Q 5.8 ....J ... \ 'l:O u 0<::.- U1- cet_~ JYv\-iS \f-v'S oo.S 313,.)_ 4~ .-J-- I ,..,........... v I 1\k-- -=~=--=-~~~-
\
.....- ~ -
,~ ------=:::
l aoe 77 of 107 l'lote: If >15m! oft1trant IS used, sample must be diluted. Reviewed by: Date reviewed [ 0'1-(D .Cfj
Page Page _1-'-'--_of 3 Total Hardness (SM 2340 C) RL = 1.0 mg CaC03/L Analyst Date analyzed bSL 1"--"'""-------1 OS-- I1--D1 Time initiated Time completed I:==~=\=Q.;:;.~~~~~~~~-_.-i D'15'1
.. T.itrant normality and multiplier determination:
Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml =0.2/E = (N x 50000)/ 50 ml sample number number (E) (acceptable range= 0.0180 ... 0.0220) =Nx 1000 rv D I Jt\1~357..... ~BS5-=1-\ 0.0 IV.D 0-Dd.O .;?o.o L aboratory contro l stan dard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range I (mg CaC031L) (_ml) ml ml ml = 90 to 110%)
*:r)X,S \a54<- 40 50 ID.O 11.0 d),t:> 0(1).0 .L..i0 (DdJLv I Hardness %RPD=
II Multiplier (mg CaCOJIL) {(S- D) /[(S+D)/2}) x 100 10.0 s D I
! atrrx sp1'k e recovery:
Reference standard number Spike value (SV) (m~ CaCOJIL)
~6Q~
Sample volume (ml) 5o Begin ml End
~-I ml Total ml
\o ..tp Multiplier 2D, 0 Spike hardness (A)
(mg CaC0JIL) I JNS51.t5ti' Sample hardness (B)
.;}15 Measured spike value (MV) f30
% R = MV / SV x 100 (mg CaCOJIL) MV=A-B (acceptable range.
I 92-(mg CaCO:JL)
- 33
- 75 to 125%)
qso~ samp.l e measurements: I Sample number Sample ID Sample volume (ml) Begin ml End ml Total ml Multiplier Hardness (mg_ CaCOJILl Blank TV=ND 5o D.() J_o.o NO I l 05*1~-bC) (should be: 0 mg CaC03/L)
..ffi }t<,. \-!w
.;<8.1 l-;)i3.1 JB.I
- 9-.0 ~.S ql.p OS.ttp.l)C) J ,J-;; .Cl 3J4 J-+.5 qo I 6.S-ol1*o~A mH-s OV I .~'7.4 ~~~ )~ A.4 <6~
'12..
ill'i
- 0'\- O'\ 5 I z. J-fr~ l4!c.J{ .Lfl.o
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I J 3 0.0 :.+.lk- -4~ OS.I2-..Q5
~~n*. o\ FbJ..wcud J 1-f.lp c,.c l.t.J. J8 ---
~~'-\.*
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. 1 1 qo., if\ I ;Jl L\.2
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54 l ge 78 of 1 lmte: If >15m! of titrant is used, sample must be diluted. Reviewed by: Date reviewed I 05 i '1-*rY1 J
Page Page _;) _ _ of 3 I Total Hardness (SM 2340 C) I Analyst I &S<..--
- RL = 1.0 mg CaC0 3/L Time initiated I -------- I Date analyzed 05. 11 . Oj Time completed ~c.
I Titrant normality and multiplier determination: Tit.:A.nt_ I reference number Normality check standar number Begin End ml
~
Total ml Normality (N) ofEDTA
=0.2/E Iacceptable range- 0.0180- 0.0220) pH Factor or Multiplier
= (Nx 50000)/ 50 ml sample
- Nx 1000
~,...
I Lba oratorv contro stan dard: Reference standard True value Sample Hardness (MV) % RS=MV /TV X 100 I number (TV) (mg CaCOJIL) volume (ml) 50 Begin ml End ml Total ml Multiplier (mg CaCOJIL) (acceptable range
=90 to 110%)
- t"N:::6 t~51p 40 f~.~ IJ.f.B 0<-D 20.0 ~ looti1.o I Hardness %RPD=
{(S- D) /[(S+D)/2]} x 100 I Multiplier (mg CaCOJIL) ml s
.2.. 2D.0 (l.l?l-f-D I j_ 4'4 M atrrx spike recovery:
I Reference standard number Spike value (SV) (mg CaCOJIL) Sample volume (ml) Begin ml End ml Total ml Multiplier Spike hardness (A) (mg CaC03/L) It--JGS IQ5t, 4u 5D loo I Sample hardness (B) IB.c !,:)3 .2- 5.2 )D.O Measured spike value (MV) %R=MV/SVx 100 (mg CaCOJIL) MV=A-B (acceptable range I \74* (rng CaC03 /L) 3lo
= 75 to 125%)
C\o"l.D Samp.I e measurements: I Sample number Sample ID Sample volume
.(ml)
Begin ml End ml Total ml Multiplier Hardness (mg CaC0 3/L)
~hnl<
TV'~~ * (should be= 0 mg CaCOy'L) 0'\0S~. 0 e- IJA ~J 101 z (I) d.3 .)_ Q?(p.3 ~. \ 2_0.\l ~.,1 0'\ OS IS .Q"'l..- l 3 ~U?3 c:!Cf.~ ~.0 bo ~ o'\OS I\* o1_ o'\os 1~. o~ O'i CJS 'S . Ci3 0'\(.)$\\.0 \ T--IA '60.t-J
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' t.\) 3.3 8.3
,)....-
\..pto
'age 79 of 1 Date: If >ISm! of titrant is used, sample must be diluted. Reviewed by: .----~-lYL-----, Date reviewed [ P5- 18--oCf
Page '1 '1
~
Page 3 of-=3=---
,;) En~~:~:tntal Tesring Solutions. Inc.
Total Hardness (SM 2340 C) RL = 1.0 mg CaCOiL Date analyzed OS-n -D'] I Analyst i?£i._ *
- Time initiated Time completed I .______
~
I r:ttrant normarzty and multiplier determinatzon:
'f,W::ant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml 0.21E - (N x 50000)1 50 ml sample number number (]!;) table range- 0.0180- 0.0220) Nx 1000
-&;c.
L aboratory controI stan dard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJil,) (acceptable range (mg CaCOJIL) (ml) ml ml ml = 90 to 110%)
- rw.:=.s ~5t.. 40 50 3.3 5.4- c.< .r 2-1.:>.'0 Lt;l. /D5~
Hardness %RPD= Multiplier (mg CaC03/L) {(S- D) I[(S+D)I2]} X 100 Z.o.o s I D I atrvc spt e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJil,) {mg CaCOJIL) (ml) ml ml ml S'NSS~\p .Y.D so '0.5 l3.:t-- 5.:>.. .z.o.u I Sample hardness (B) Measured spike value (MV) IDO
% R = MV I SV x 100 (mg CaC031L) MV=A-B (acceptable range I
' L?!i-(mg CaCOJIL)
&c
= 75 to 125%)
C\o<>Lo samp,l e measurements: I Sample number Sample ID Sample volume (ml} Begin ml End ml Total ml Multiplier Hardness (mg CaCOJil,) TV=ND Blank .... I
,____ (should be - 0 mg CaCOJIL)
OC\OSIS. 0~ IfA ~v-> i..JI Jv 3 6'0 13.1' llo.tJ; ~q )..O.v 5'6
~O~OSdl...'lC IJ.4. l)..tFQ)j z_g 1
/lg.(p Jl..q 5.3 17 I ~10 ~ .!Ljz_
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,J_ ~*;;)._
r-- I ____..,. /
-- ~ --
/
L .. ( ~f.-*--
~~L-lge 80 of 1Qdle: If > l Sml of titrant is used, sample must be diluted. Reviewed by: 1~. . .*_ _K_i.:JA__ ___ _J Date reviewed!.__-=Q)=--/.__A_-D-"--'1_-- - '
Sequoyah Nuclear Plant Biomonitoring May 12-19, 2009 Appendix D Reference Toxicant Test and Control Chart Page 81 of 107
*** Pimephales promelas i~
Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc.
~ Environmental Testing Solutions, Inc.
USEPA Control Limits (+/- 2 Standard Deviations) 1.0 0.8 0.6 0.4 1.2 u
~
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.U$Ef4 WarningandConttolLimits (75 1hand9dh PercentileCVs) ..
~
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t-- 1.2 Laboratory Warning and Control Limits (Idh and 25th Percentile CVs) l.O 0.8 0.6 0.4 Test date 7-day IC25 = 25% inhibition concentration. An estimation ofthe concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC 25 ) Warning Limits (mean IC 25 +/- SA 10 or SA 75 ) Control Limits (mean IC 25 +/- SA 25 , SA 90 , or 2 Standard Deviations) p~~~~~----------------------------------------------------------~-----------~
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Pimephales promelas Chronic Reference Toxicant Control Chart State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day IC,. CT s Control Limits s._" Warning Limits s..,. Control Limits s...,. Warning Limits Suo Control Limits CV (gfLKCI) (giL KCl) Cf- 2S CT+2S cr- s._,. CT + s._ 10 cr- s._,. cr + SA.ls cT- sA.7s cr + sA.7s CT- SA.Oo cr +SA... 10-28-08 0~ 2 11-04-08 0~ 0.73 0.08 0.56 0.90 0.09 0.64 0.82 0.15 0.58 0.88 0.28 0.45 1.01 0.33 0.40 1.06 0.11 12-09-08 0~ on 0.06 0.59 0.84 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.39 1.04 0.09 4 01-06-09 ~M 0.73 0.05 0.62 0.84 0.09 0.64 0.81 0.15 0.57 0.88 0.28 0.45 1.00 0.33 0.40 1.05 0.07 5 01-13-09 0~ 0.75 0.06 0.62 0.87 0.09 0.66 0.83 0.16 0.59 0.90 0.28 0.46 1.03 0.34 0.41 1.08 0.08 6 01-15-09 ~n 0.75 0.06 0.64 0.86 0.09 0.66 0.84 0.16 0.59 0.91 0.29 0.47 1.04 0.34 0.41 1.09 0.08 7 01-21-09 ~n 0.75 0.05 0.65 0.86 0.09 0.66 0.85 0.16 0.60 0.91 0.29 0.47 1.04 0.34 0.42 1.09 0.07 8 02-03-09 ~~ 0.76 0.05 0.66 0.86 0.09 0.67 0.85 0.16 0.60 0.92 0.29 0.47 1.05 0.34 0.42 1.10 0.07 9 02-10-09 ~D o.n 0.05 0.66 0.87 0.09 0.67 0.86 0.16 0.61 0.93 0.29 0.48 1.06 0.34 0.42 1.11 0.07 10 02-17-09 ~~ 0.74 0.09 0.56 092 0.09 0.65 0.83 0.16 0.59 0.90 0.28 0.46 1.02 0.33 0.41 1.08 0.12 11 02-24-09 ~w 0.73 0.10 0.54 0.92 0.09 0.64 0.82 0.15 0.58 0.88 0.28 0.45 1.01 0.33 0.40 1.06 0.13 12 02-25-09 ~n 0.73 0.09 0.55 0.91 0.09 0.64 0.82 0.15 0.58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.12 13 02-27-09 ~n 0.73 0.09 0.56 0.91 0.09 0.64 0.82 0.15 0.58 0.88 0.28 0.45 1.01 0.33 0.40 1.06 0.12 14 03-03-09 Q~ 0.73 0.08 0.56 0.90 0.09 0.64 0.82 0.15 0.58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.11 15 03-10-09 0~ 0.74 0.08 0.57 0.90 0.09 0.65 0.82 0.15 0.58 0.89 0.28 0.46 1.02 0.33 0.41 1.07 0.11 16 03*10-09 Qn 0.74 0.08 0.58 0.90 0.09 0.65 0.83 0.16 0.58 0.89 0.28 0.46 1.02 0.33 0.41 1.07 0.11 17 04-14-09 QW 0.74 0.08 0.58 0.89 0.09 0.65 0.82 0.15 0.58 0.89 0.28 0.46 1.02 0.33 0.40 107 0.11 18 04-21-09 QW 0.74 0.08 0.59 0.89 0.09 0.65 0.83 0.16 0.58 0.90 0.28 0.46 1.02 0.33 0.41 107 0.10 19 05-05-09 Q~ 0.74 0.08 0 59 0.89 0.09 0.65 0.83 0.15 0.58 0.89 0.28 0.46 1.02 0.33 0.41 107 0.10 20 05-12-09 Q~ 0.74 O.o7 0.59 0.89 0.09 0.65 0.83 0.15 0.58 0.89 0.28 0.46 1.02 0.33 0.41 1.07 0.10 Note: 7-d IC, = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pil)lephales growth for the test population. CT = Central tendency (mean !C15 ). S = Standard deviation of the IC25 values. Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC, values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. Suo= Standard deviation corresponding to the lOth percentile CV. (SA 10 = 0.12) Su 5 = Standard deviation corresponding to the 25th percentile CY. (SA 1, = 0.21) USEPA Control and Warning Limits s*., = Standard deviation corresponding to the 75th percentile CY. ( SA.7l = 0.38) s.... = Standard deviation corresponding to the 90th percentile CV. (SA 90 = 0.45) CV = Coefficient of variation of the IC 15 values. USEPA 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003 US EnvironmentaJ Protection Agency, Cincinnati, OH. Organi.Ym.s obtained from Aquatox, Inc. atox05-12-09
Precision of Endpoint Measurements Pimephales promelas Chronic Reference Toxicant Data Environmental Testing Solutions, Inc. Control Control Mean Test number Test date Survival Growth CT cv CT MSD PMSD CT for Control Growth for Control (%) (mg/larvae) (%) (%) forPMSD (%) (mg!larvae) Growth CV (%) 10-28-08 100 0.586 6.5 0.10 17.8 2 11-04-08 97.5 0.643 0.614 2.5 4.5 0.12 18.7 18.2 3 12-09-08 100 0.522 0.583 2.4 3.8 0.05 10.0 15.5 4 01-06-09 100 0.644 0.599 3.2 3.6 0.09 13.3 14.9 5 01-13-09 97.5 0.556 0.590 9.7 4.9 0.06 11.2 14.2 6 01-15-09 100 0.707 0.610 6.5 5.1 0.09 13.3 14.1 7 01-21-09 97.5 0.594 0.607 4.9 5.1 0.07 11.5 13.7 8 02-03-09 100 0.671 0.615 8.4 5.5 0.06 9.6 13.2 9 02-10-09 100 0.591 0.613 5.1 5.5 0.09 15.7 13.5 10 02-17-09 100 0.804 0.632 3.3 5.2 0.06 6.8 12.8 11 02-24-09 100 0.744 0.642 2.5 5.0 0.10 13.0 12.8 12 02-25-09 100 0.659 0.643 8.2 5.3 0.07 11.1 12.7 13 02-27-09 100 0.578 0.638 6.8 5.4 0.08 13:1 12.7 14 03-03-09 100 0.664 0.640 8.1 5.6 0.08 12.3 12.7 15 03-10-09 100 0.661 0.642 6.8 5.7 0.09 12.9 12.7 16 03-10-09 100 0.696 0.645 8.8 5.9 0.09 12.6 12.7 17 04-14-09 100 0.671 0.646 10.3 6.1 0.08 11.4 12.6 18 04-21-09 100 0.642 0.646 8.3 6.2 0.07 11.2 12.5 19 05-05-09 100 0.805 0.655 7.9 6.3 0.07 8.4 12.3 20 05-12-09 100 0.717 0.658 7.0 6.4 0.06 8.9 12.1 Note: CV =Coefficient of variation for control growth. Lower CV bound determined by USEPA (lOth percentile)= 3.5o/o. Upper CV bound determined by USEPA (90th percentile)= 20% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10m percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile)= 30%. CT =Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Umkrstanding ami AccoWJting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200 la, 200 I b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute \Vhole Effluent Toxicity Test Methods, Volumes l and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtained from Aquatox, Inc. atox05-12-09 Page 84 of I 07
Pimephales promelas Chronic Reference Toxicant Control Chart
*u Precision of Endpoint Measurements
*~ Environmental Testing Solutions, Inc.
Organism Source: Aquatox, Inc.
-- ~ 1.00
-=~ ~
E: Q
~
0.75
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Co-' ~
..... 0.50 Cl .........
Cl Q b.IJ u ._, 6 0.25 - - - - *- *-**-**- *- - -**-* -* *--*- *- - - -* -**- *-*- - *- USEPA Acceptance Criteria(> 0.25 mg per surviving larvae)
-- -= ';!. 30 - - * - -**-**-**-**-*- *- *- - - - -**- - * - - - -**-**- - *- -.
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10 Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
-- Central Tendency (mean Control Growth, CV, or PMSD) p ge SS of tO'fontrol Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)
-----------------~
Page 1 of 5 iETS
} Enviranmemal Tasting Solutions- lnc.
Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas
. PpKClCR Test Number: ___iU.
Dilution preparation information: Comments: KCl Stock INSS number: INS~ loL\ \ Stock preparation: 50 g KCL'L: Dissolve 50 g KCI in 1-L Deionized water Dilution prep (mgfL) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Test information: Organism age: t.o. ~~ ~\l<lS 01...1;:) Randomizing template: l61leeN Date and times organisms OS-\\*0~ \loCO Incubator number and were born between: shelf location: 2>1:> Organism source: ~ &P...\t.\\ ~,p 0'5.*\\- 0~ Artemia CHM number: It~ l-\ l\'-11.\ Transfer bowl infonnation: pH= SU Temperature= oc Date I Time in: o S*t'\*ul\ \1..0b 1-~'- "1-4 .lo Date I Time out: o~*2o-cA IZ10 Average transfer volume: Total drying time: z"\.1.5_~11<<! O.'l~S~ Initial oven temperature: ~o*_c.. Final oven temperature: lo\
- t.
Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW batch used 0 r 2 II 3 4 Ii 5 6 7 I I I ~ge 86 of 107
Page 2 of5 iETS
) Environmtnta!Testfng ScHuriDns, Inc.
Species: Pimephales promelas PpKCICR Test Number: ..J.l1.., Survival and Growth Data Day Control 450mgKCVL 601!_!ng KCVL A B c D E F G H I J K L 0 ID 10 10 /0 IO IO 10 /0 IO /0 10 ID 1 It> 10 If:> to to /0 IC /Q (C) IO IO 10 2
/0 ID /Q IO 10 IQ 10 I Q 10 I(J 10 ID 3
IC to ID lO ID /D 10 10 IC IO 10 10 4 ro /0 to IO /0 /0 ID tO 10 10 10 10 5 I D 10 10 IO /0 to tO /0 10 IQ 10 10 6
/0 /D /0 10 to IO /0 10 IO /0 10 10 7
/Q IO IO IQ 10 10 /0
,J.. 10 10 10 10 A= Pan weight (mg)
Tray color code:: Dro.!f Analyst: r"L.F 14.11 13.b~ \~X~ IJ.D{ j\f. q~ 14.lf. 1~.31 )~.~tt lVII o. 1~ \~.43 llJ.qJ Date: OS' *(#-*lA B =Pan+ Larvae weight (mg) f\Lr
;).1.4b ;J.D:~8 J.a..lt ttt-.4+ 1~1. 61 ~~.1q i)o.d-'1 a.t.oJ J..o.'8~ Jq,q3 J.d..~ l'{.l!l>
Analyst: Date: b ~. 1- b - ll~ C =Larvae weight (mg)
=B-A 1#. (.;'\ ,.2.\ i.i'5 (a.'\ "Z.. '1.1'2 t,.S't. ~'I! lo.IY 1-"'H 1. ,g- I...R~ b.'\1 Weight per initial number
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Average Percent weight per reduction initial from control 0.11"1 0 .IP\s.~ lo *' '1. 0.11D 1. () 7. number of (%) larvae (m2) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk = sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: $-- Comments: age 87 of 107
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~ En~lronm~nt.l Tit.sting Solutions, Inc.
Page 3 of5 Species: Pimephales promelas PpKCICR Test Number: l& 'L Survival and Growth Data Day 750 m~ KCI/L 900 m~ KCIIL 1050 M! KCI/L M N 0 p Q R s T u v w X 0 10 10 IQ IO /0 10 /0 /() ID /0 10 10 1 y.. '{ck. ~ "leA. sJ. SJ,. ~ '{J. ICJ &'J.. IO /0 \ \., 1 s s s ~ 2
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1.. 1 Coac.eotratioa (DIWL Rtlpliute Initial aumbcr of Fiaal .. mbel'of A - Pu weig*t (mg) B "" Paa + Larval! L*rvac wei~ I (mg) Wci.pt I Suniviat: Mtaa wcielltl Cod&clcat ofvariatioa Weigllt /laitial *umber Meaas.,vinJ Meu weiclt.tllaitial Cill!llroeat of Peru.ot rulutioa from KO) larvae Janru weiJ:bt(mg) =A.-8 oamher of larvae (mg) Surviviag uuaaber of (M_....,,..,... rtlriof oft.rv.. (mg) (%) lumber of larvae variatioa (%) eootrol (%) t.rvae(mg) .....,ruf-".c:)(Ye) (Gig) A 10 10 14.77 21.46 6.69 0.669 0.669 B 10 10 13.07 20.28 7.21 0.721 0.721 Control 0.717 7.0 100.0 0.717 7.0 Not applicable c lit 10 14.33 22.18 7.85 0.785 0.785 D 10 10 13.05 19.97 6.92 0.692 0.692 E 10 10 14.49 21.61 7.12 0.712 0.712 F 10 10 14.27 20.79 6.52 0.652 0.652 450 0.686 3.8 97.5 0.669 6.7 6.7 G 10 10 13.31 20.29 6.98 0.698 0.698 H 10 9 14.89 21.03 6.14 0.682 0.614 I 10 10 13.41 20.82 741 0.741 0.741 J 10 10 12.75 19.93 7.18 0.718 0.718 600 0.710 3.6 100.0 0.710 3.6 1.0 K 10 10 15.43 22.26 6.83 0.683 0.683 L 10 10 12.93 19.90 6.97 0.697 0.697 M 10 8 14.44 19.90 5.46 0.683 0.546 N 10 8 14.92 19.87 4.95 0.619 0.495 750 0.667 5.1 80.0 0.533 5.1 25.6 0 10 8 14.85 20.20 5.35 0.669 0.535 p 10 8 14.01 19.58 5.57 0.696 0.557 Q 10 6 13.62 17.95 4.33 0.722 0.433 R 10 5 13.11 16.11 3.00 0.600 0.300 900 0.654 7.7 52.5 0.345 23.6 51.8 s 10 4 14.76 17.32 2.56 0.640 0.256 T 10 6 12.64 16.56 3.92 0.653 0.392 u 10 3 14.23 16.47 2.24 0.747 0.224 1050 v 10 4 14.11 17.39 3.28 0.820 0.733 11.0 0.328 37.5 0.275 16.9 61.7 w 10 4 13.81 16.77 2.96 0.740 0.296
- ---- ----~ 10 4 15.13 17.63 2.50 0.625 0.250 Dunnett's MSD value: 0.064() MSD= Minimwn Significant Difference PMSD: 8.9 PMSD= Percent Minimum Significant Diffenonce PMSD is a measure of test precision. The PMSD is the minimwn percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (lOth percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile)= 30%. Lower and upper PMSD bounds were detennioed from the lOth and 90th percentile, respectively. ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA. 2001a; USEPA, 2001b). US EPA 200 Ia, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-tenn Chronic and Acute Whole Effluent Toxicity Test Methods, Volwnes I and 2-Appendix. EPA-821-B-{11-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Orgamsms obtamedjrom Aquarox, Inc. atoxOS-12*09
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Larval Fish Growth and Survival Test-7 Day Survival Start Date: 5112/2009 Test lD: PpKClCR Sample ID: REF-RefToxicant End Date: 511912009 Lab lD: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Con e-m giL I 2 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 0.9000 600 1.0000 1.0000 1.0000 1.0000 750 0.8000 0.8000 0.8000 0.8000 900 0.6000 0.5000 0.4000 0.6000 1050 0.3000 0.4000 0.4000 0.4000 10.00 10.00
*900 0.5250 0.5250 0.8106 0 6847 0.8861 11.892 4 10.00 10.00 19 40
*!050 0 3750 0.3750 0.6584 0.5796 0.6847 7.979 4 10.00 10.00 25 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) 0.81382352 0.884 -1.2632773 2.14787125 Equality of variance cannot be oonfirmed Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 600 750 670.820393 Treatments vs D-Control Maximum Likelihood-Pro bit Parameter Value SE 95% Fiducial Limits Control Chi-Sq__ Critical P-value Mu Sigma Iter Slope 8.14828973 1.29114829 5.61763904 10.6789404 0 6.87582293 7.81472778 0.08 2.97727854 0.12272514 4 Intercept -19.259728 3.78378062 -26.675938 -11.843518 TSCR Pomt Probits mg/L 95% Fiducial Limits ECOI 2.674 491.787145 380.202898 566.020355 EC05 3.355 596.229761 500.029379 659.146411 EC10 3.718 660.693371 577.154041 716.766606 ECI5 3.964 708.079653 634.372816 760.17228 EC20 4.158 748.153069 682.308654 798.356768 EC25 4.326 784.335178 724.514185 834.704503 EC40 4.747 883.459176 829.997827 948.216155 EC50 5.000 949.026949 890.476916 1015.58115 EC60 5.253 1019.46098 949.626271 1137.82792 EC75 5.674 1148.30007 1049.26122 1340.14047 EC80 5.842 1203.83407 1090.1848 1431.97553 EC85 6.036 1271.96449 1139.27255 1547.85189 EC90 6.282 1363.19229 1203.47434 1708.07612 EC95 6.645 1510.57893 1304.3074 1978.13621 EC99 7 326 1831 38628 1514 60449 2608.95733 Organisms obtainedfrom Aquatox, Inc. atoxOS-12-09
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Larval Fish Growth and Survival Test-7 Da~ Growth Start Date: 5/12/2009 TestlD: PpKClCR SampleiD: REF-Ref Toxicant End Date: 5/19/2009 Lab 10: ETS-Envir. Testing Sol. Sample Type: KCL-Potassiwn chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Conunents: Conc-m!iL I 2 3 4 D-Control 0.6690 0.7210 0.7850 0.6920 450 0.7120 0.6520 0.6980 0.6140 600 0.7410 0.7180 0.6830 0.6970 750 0.5460 0.4950 0.5350 0.5570 900 0.4330 0.3000 0.2560 0.3920 1050 0.2240 0.3280 0.2960 0.2500 Transform: Untransfonned !-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7168 1.0000 0.7168 0.6690 0.7850 7.008 4 0.7168 1.0000 450 0.6690 0.9334 0.6690 0.6140 0.7120 6.687 4 1.627 2.180 0.0640 '0.6894 0.9618 600 0.7098 0.9902 0.7098 0.6830 0.7410 3.567 4 0.239 2.180 0.0640 0.6894 0.9618 750 0.5333 0.7440 0.5333 0.4950 0.5570 5.070 4 0.5333 0.7440 900 0.3453 0.4817 0.3453 0.2560 0.4330 23.589 4 0.3453 0.4817 1050 0.2745 0.3830 0.2745 0.2240 0.3280 16.925 4 0.2745 0.3830 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97055012 0.805 0.28149786 -0.6879762 Bartlett's Test indicates equal variances (E = 0.55) 1.1904434 9.2103405 H~othesis Test (!-tail, 0.05~ NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 600 >600 0.06396215 0.08923913 0.00265975 0.00172172 0.2649911 2,9 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95%CqExe) Skew
!COS 608.13 149.72 0.00 651.16 -0.8286
!CIO 642.56 54.75 277.41 687.07 -3.5954 ICI5 676.99 19.21 608.59 724.00 -0.4242 IC20 711.43 18.63 652.82 760.37 -0.2084 IC25 745.86 17.03 695.14 791.43 -0.2232 IC40 832.34 23.14 784.14 931.75 0.9228 IC50 889.53 36.16 825.86 1033.39 0.8216 Organisms obtained from Aquatox, Inc. atox05-12-09
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Species: Pimephales promelas PpKCICR Test Number: \8"2._ 1050 mg KCVL STOCK f IPage 92 of 107
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Species: Pimephales promelas PpKClCR Test Number: \&-'2-1050 mg KCI!L STOCK 93 of 107
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Ceriodaphnia dubia Chronic Reference Toxicant Control Chart
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1.14 USEPA Control Limits (+/- 2 Standard Deviations) 1.12 1.10 1.08 --- - ----- 1.06 1.04 1.02 u z~ 2.5 2.0 USEPA Warning and Control Limits (75th and 9dh Percentile CVs) u
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Central Tendency (mean IC25 ) Warning Limits (mean IC 25 +/- SAlO or SA. 75 ) Control Limits (mean IC 25 +/- SA25' SA 90 , or 2 Standard Deviations) P a~~7~u* 1 ",..
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State and USEP A Laboratory Laboratory USEPA USEPA Test 7-day IC25 CT s Control Limits SA.to Warning Limits SA.2s Control Limits SA.1s Warning Limits SA.90 Control Limits cv (giL NaCI) (giL NaCI) CT- 2S CT + 2S CT- SA.IO CT + SA,IO CT- SA.2S CT + SA.l5 CT- SA.75 CT + SA.75 CT- S 00 CT + SA.'!o 1.09 2 1.09 1.09 0.00 1.09 1 09 Q~ 1.00 1.18 0.19 0.90 1.28 0.49 0.60 1.58 0.68 0.41 1.77 0.00 3 1.07 1.08 0.01 1.06 1.11 om 1.00 1.17 0.18 0.90 1.27 0.49 0.60 1.57 0.67 0.41 1.76 0.01 4 1.07 1.08 0.01 1.06 1.10 0~ 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.01 5 1.07 1.08 0.01 1.06 1.10 0~ 0.99 1.16 0.18 0.89 1.26 0.49 0.59 1.56 0.67 0.41 1.75 0.01 6 1.06 108 0.01 1.05 LIO 0~ 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.01 7 Ill 1.08 0.02 105 1.11 0~ 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.02 8 107 1.08 002 105 1.11 0~ 0.99 1.16 0.18 0.89 1.26 0.49 0.59 1.56 0.67 0.41 1.75 0.01 9 I 09 1.08 0.02 1.05 1.11 0~ 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.01 10 I 10 1.08 0.02 1.05 111 0.~ 1.00 1.17 0.18 0.90 1.27 0.49 0.59 1.57 0.67 0.41 1.75 O.QJ II I 09 1.08 0.02 105 1.11 0.~ 1.00 Ll7 0.18 0.90 1.27 0.49 0.60 1.57 0.67 0.41 1.75 0.01 12 I 07 1.08 0.01 1.05 Lll 0~ 1.00 1.17 0.18 0.90 1.27 0.49 0.59 1.57 0.67 0.41 1.75 O.Dl 13 110 1.08 0.01 1.05 Ill 0~ 1.00 1.17 0.18 0.90 1.27 0.49 0.60 1.57 0.67 0.41 1.75 O.QJ 14 106, 1.08 0.02 1.05 1.11 om 0.99 117 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.01 15 105 1.08 0.02 1.05 1.11 0~ 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 175 0.02 16 1.05 1.08 0.02 104 Ll1 0.~ 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.75 0.02 17 107 1.08 0.02 1.04 1.11 0~ 0.99 Ll6 0.18 0.89 126 0.48 0.59 1.56 0.67 0.41 1.74 0 02 18 107 1.08 0.02 104 Ll1 0.~ 0.99 116 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.02 19 109 1.08 0.02 1.04 1.11 Q~ 0.99 1.16 0.18 0.89 126 0.48 0.59 1.56 0.67 041 1.75 0.02 20 107 1.08 0.02 1.04 Lll 0~ 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.02 IC 25 = 7-day 25% inhibition concentratiOn. An estimation of the concentration of sodiwn chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC 21 ). S = Standard deviation of the ICzs values. ILabdratorv Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint SA.IO = Standard deviation corresponding to the I o'" percentile CV. (SAw= 0.08) SA.lS = Standard deviation corresponding to the 25th percentile CV (SA 23 = 0.17) Control and Warning Limits SA.75 = Standard deviation corresponding to the 75°' percentile CV. (SA 75 = 0.45) SA.'!U = Standard deviation corresponding to the 90th percentile CV. (SA. 90 = 0.62) CV = Coefficient of variation of the IC 23 values. and Accounting for Method Variability in Whole Effiuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. 05-05-09
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Precision of Endpoint Measurements Ceriodaphnia dubia Chronic Reference Toxicant Data Environmental Testing Solutions, Inc. Test Control Control Mean number Test date Survival Reproduction CT cv CT MSD PMSD CT for Control Mean for Control (%) (offspring/female) Reproduction (%) Reproduction (%) for PMSD (%) (offspring/female) CV(%) 1 01-08-08 100 30.3 6.6 2.2 7.3 2 01-08-08 100 31.8 31.1 4.9 5.7 2.4 7.7 7.5 3 02-05-08 100 31.3 31.1 6.4 6.0 2.8 9.1 8.0 4 03-04-08 100 31.5 31.2 8.0 6.5 2.7 8.5 8.1 5 04-08-08 100 29.6 30.9 7.7 6.7 2.6 8.8 8.3 6 05-06-08 100 32.1 31.1 6.3 6.6 2.5 7.9 8.2 7 06-03-08 100 30.5 31.0 7.9 6.8 3.0 9.9 8.4 8 07-08-08 100 30.9 31.0 6.7 6.8 2.4 7.7 8.4 9 08-05-08 100 29.4 30.8 7.9 6.9 2.2 7.6 8.3 10 09-09-08 100 28.3 30.6 8.5 7.1 3.0 10.5 8.5 11 10-07-08 100 32.4 30.7 7.2 7.1 2.7 8.4 8.5 12 11-04-08 100 32.3 30.9 5.1 6.9 2.2 6.8 8.3 13 11-04-08 100 31.5 30.9 6.2 6.9 2.4 7.7 8.3 14 12-02-08 100 31.5 31.0 4.6 6.7 2.5 8.0 8.3 15 01-06-09 100 33.2 31.1 4.0 6.5 2.0 6.1 8.1 16 02-03-09 100 31.4 31.1 6.6 6.5 1.9 6.0 8.0 17 02-24-09 100 32.2 31.2 6.0 6.5 1.8 5.7 7.9 18 03-10-09 100 31.9 31.2 5.8 6.5 2.6 8.2 7.9 19 04-14-09 100 33.9 31.4 6.7 6.5 2.0 5.8 7.8 20 05-05-09 100 33.6 31.5 4.3 6.4 2.3 6.8 7.7 Note: CV = Coefficient of variation for control reproduction. Lower CV bound determined by USEPA (lO'h percentile)= 8.9%. Upper CV bound determined by USEPA (90th percentile)= 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (IO'h percentile) = 13%. Upper PMSD bound determined by USEPA (90 1h percentile)= 47%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Enviro_tlmentaJ Protection Agency, Cincinnati OH. ~-~~~ ______________ _ USEPA. 2001 a, 200 I b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. 05-05-09 Page 96 of 107
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Ceriodaphnia duhia Chronic Reference Toxicant Control Chart
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Page 1 of6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia CdNaCLCR #: 8'\ Dilution preparation information: Comments: NaCI Stock lNSS number: IN$ (.t...( 0 Stock preparation: 100 g NaCl/1 (dissolve 50 g NaCl in 500 ml deionized water) Dilution prep (mg!L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (rnL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test organism source information: Test information: Organism age: < 24-hours old Randomizing template color: ('} .t At-:6 e_ Date and times organisms were born os-05-o'\ ot.'-\S Tt>ca5S Incubator number and shelf between: location: 2..&\ Culture board: o'-\*"2.8-cA Replicate number: 1 1 2 1 3 4 1 s 1 6 1 1 1 s 1 9 1 10 YWT batch: Culture board cup number: "1.1 ~ I'\ lO I l"'lj I'-'l ,, I 2\ I n..Ll.l! a'"l~O'l--!1\ Transfer bowl information: pH ,.'-('\ su Temperature "'l.S* 2. oc SelenrJstrum batch: Daily renewal information: I Day Date Test initiation and feeding, renewal and feeding, or termination time MHSW batch used Analyst I 0 2 I 3 4 I 5 6 7 I I Control information: Acceptance criteria Summary of test endpoints:
% of Male Adults: D7~ 520% 7-day LC 50 )11./00 I %Adults having 3'd Broods:
% Mortality:
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CONTROL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 D _a_ 0 0 0 0 Adult mortality '"\ '- '- '- \._ L L_ ~ l_ L 2 Young produced D a 0 () a () D 0 0 () llIJ Adult mortality L \._
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% Mortality: I Dl.
Mean Offspring/Female: I .33.1.. 600 mg NCIIL a S urvtva
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Page 3 of6 Species: Ceriodaphnia dubia CdNaCLCR #: g(\ 800 mg Na CI/L Survival and Reproduction Data
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r Page 4 of6 r Species: Ceriodaphnia dubia CdNaCLCR#: ~g~q~ 1200 mg Na Cl/L SurvivaI an dRepro ductzon D ata Replicate number Day 1 2 3 4 5 6 7 8 9 10 f I Young produced '() 0 (') 0 n 0 0 0 L. I) 0 Adult mortality
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( on rol 1000 mg NaCI/L Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 4 5 5 6 5 5 6 4 4 50 4 6 4 6 5 4 5 5 5 4 5 49 5 13 13 12 12 12 14 12 12 13 13 126 5 13 II 12 10 10 13 II II 12 12 115 6 0 17 0 0 0 0 16 0 17 15 65 6 0 0 0 0 0 17 0 0 0 15 32 7 17 0 17 14 16 16 0 15 0 0 95 7 13 14 12 15 13 0 14 14 16 0 Ill To al 36 34 34 31 34 35 33 33 34 32 336 Total 32 29 30 30 27 35 30 30 32 32 307 6~0 ~g NaCl/L 1200 mg NaCUL Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 l 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 41 6 4 5 5 5 6 5 6 6 5 53 4 5 5 5 4 4 5 4 4 4 4 44 5 12 13 14 14 14 13 13 II 13 13 130 5 5 5 7 3 7 5 5 6 5 5 53 6 0 18 0 16 0 0 18 0 0 0 52 6 0 0 0 0 0 0 0 I 0 6 7 7 17 0 15 0 18 15 0 16 16 16 113 7 5 9 6 5 8 6 4 7 5 0 55 To al 35 35 34 35 37 34 36 33 35 34 348 Total 15 19 18 12 19 16 13 18 _I_!_~ __ 1_~9__ 8~0 ~gNaCUL 1400 mgNaCl/L Replicate number Replicate number Da~ Total Day Total
! I 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 5 5 5 6 5 5 5 49 4 5 3 4 2 5 4 3 3 3 2 34 5 13 13 II 13 II 12 13 12 12 12 122 5 4 0 3 3 5 5 6 0 0 4 30 6i 0 0 0 0 0 0 17 0 16 16 49 6 0 4 0 0 0 0 0 2 0 0 6 7 16 16 15 15 17 18 0 15 0 0 112 7 3 1 0 0 5 2 0 0 0 3 14 To~al 34 33 30 33 33 35 36 32 33 33 332 Total 12 8 7 5 15 11 9 5 3 9 84
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Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaOCRN89 Test dates: May 05-12,2009 Reveiwed by:
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IPolicate number I I Survival (%) I Average reproduction (offspring/female) I Coefficient of variation (Vo) I Percent reduction from control (%) 2 I 3 I 4 5 6 7 8 9 10 34 I 34 I 31 34 35 33 33 34 32 100 33.6 4.3 Not applicable 35 I 34 I 35 37 I 34 36 33 35 34 100 34.8 3.3 -3.6 34 I 33 I 30 I 33 33 I 35 36 32 33 33 100 33.2 4.9 1.2 100(1 I 32 I 29 I 30 I 30 27 I 35 30 30 32 32 100 30.7 7.0 8.6 I 15 I 19 I 18 I 12 19 I 16 13 18 14 15 100 15.9 15.8 52.7 I 12 I 8 I 7 I 5 15 I 11 9 5 3 9 90 8.4 43.2 75.0 Dunnett's 2.291 MSD= Minimum Significant Difference PMSD: 6.8 PMSD= Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (!Oih percentile) = 13%. Upper PMSD bound determined by USEPA (901h percentile)= 47%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (tJSEPA, 200la; tJSEPA, 2001b). USEP A 2~0 I a,!200 I b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-0 1-004 and EPA-821-B-0 1-005. US Envirm1men~al Protection Agency, Cincinnati, OH.
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Ceriodaplmia Survival and Reeroduction Test-R=eroduction 1 Start Date: 5/5/2009 TestlD: CdNaClCR Sample ID: REF-Ref Toxicant i End Date: 5/12/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaplmia dubia Corrunents: Conc-m~L I 2 3 4 5 6 7 8 9 10 0-Control 36.000 34.000 34.000 31.000 34.000 35.000 33.000 33.000 34.000 32.000 600 35.000 35.000 34.000 35.000 37.000 34.000 36.000 33.000 35.000 34.000
&00 34.000 33.000 30.000 33.000 33.000 35.000 36.000 32.000 33.000 33.000 1000 32.000 29.000 30.000 30.000 27.000 35.000 30.000 30.000 32.000 32.000 1200 15.000 19.000 18.000 12.000 19.000 16.000 13.000 18.000 14.000 15.000 1400 12.000 8.000 7.000 5.000 15.000 11.000 9.000 5.000 3.000 9.000 Transform: Untransformed Rank !-Tailed Isotonic Conc-mg!L Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 33.600 1.0000 33.600 31.000 36.000 4.255 10 34.200 1.0000 600 34.800 1.0357 34.800 33.000 37.000 3.262 10 129.50 75.00 34.200 1.0000 800 33.200 0.9881 33.200 30.000 36.000 4.877 10 95.50 75.00 33.200 0.9708
*1000 30.700 0.9137 30.700 27.000 35.000 7.045 10 68.00 75.00 30.700 0.8977
*1200 15.900 0.4732 15.900 12.000 19.000 15.814 10 55.00 75.00 15.900 0.4649
*1400 8.400 0.2500 8.400 3.000 15.000 43.179 10 55.00 75.00 8.400 0.2456 Auxiliary Tests Statistic Critical Skew Kurt Kolrnogorov 0 Test indicates normal distribution (p > 0.01) 0.81542361 1.035 0.21736896 0.95569016 Bartlett's Test indicates unequal variances (p = 8.87E-03) 15.3749275 15.0862722
! Hypothesis Test (!-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 800 1000 894.427191 Treatments vs D-Control Linear interpolation (200 Resamp1es)
Point mg/L SO 95% CL Skew IC05 856.8 42.6664107 764.087662 941.198214 0.0444 IC10 993.6 29.0004806 921.551731 1015.99639 -0.8830 1, JC15 1022.02703 8.689644 1004.45361 1037.76034 -0.3344 1C20 1045.13514 7.64509171 1029.66986 1060.12003 -0.0590 IC25 1068.24324 7.05805813 1055.32988 1081.38741 0.0234 IC40 1137.56757 7.32664661 1123.52221 1151.44087 0.2385 lC50 1183.78378 9.03530003 1167.78076 1201.21731 0.2331 05-05-09
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Usc: d for PMSD calculation only. Ceriodaphnia Survival and Reproduction Test-Reproduction Sta ~Date: 5/5/2009 Test ID: CdNaClCR Sample ID: REF-Ref Toxicant Sample Type: 1 Enc Date: 5/12/2009 Lab ID: ETS-Envir. Testing Sol. NACL-Sodium chloride San pie Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Co ~ments: Cc nc-mg/L I 2 3 4 5 6 7 8 9 10 10-Control 36.000 34.000 34.000 31.000 34.000 35.000 33.000 33.000 34.000 32.000 600 35.000 35.000 34.000 35.000 37.000 34.000 36.000 33.000 35.000 34.000 I 800 34.000 33.000 30.000 33.000 33.000 35.000 36.000 32.000 33.000 33.000 1000 32.000 29.000 30.000 30.000 27.000 35.000 30.000 30.000 32.000 32.000 1200 15.000 19.000 18.000 12.000 19.000 16.000 13.000 18.000 14.000 15.000 1400 1'2.000 8.000 7.000 5.000 15.000 11.000 9.000 5.000 3.000 9.000 Transform: Untransformed !-Tailed Cc nc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD [ -Control 33.600 1.0000 33.600 31.000 36.000 4.255 10 600 34.800 1.0357 34.800 33.000 37.000 3.262 10 -1.198 2.287 2.291 800 33.200 0.9881 33.200 30.000 36.000 4.877 10 0.399 2.287 2.291
*1000 30.700 0.9137 30.700 27.000 35.000 7.045 10 2.895 2.287 2.291
*1200 15.900 0.4732 15.900 12.000 19.000 15.814 10 17.667 2.287 2.291
*1400 8.400 0.2500 8.400 3.000 15.000 43.179 10 25.153 2.287 2.291 Au iliaryTests Statistic Critical Skew Kurt Ko mogorov D Test indicates normal distribution (p > 0.0 t) 0.81542361 1.035 0.21736896 0.95569016 Bar lett's Test indicates unequal variances (p = 8.87E-03) 15.3749275 15.0862722 HyJ othesis Test ( 1-tait, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Du nett's Test 800 1000 894.427191 2.29089729 0.0681814 7 1241.68 5.01851852 6.4E-36 5, 54 Tr4tments vs D-Control 05-05-09
Page5of6 I Species: Ceriodaphnia dubia CdNaCLCR #: 8'\ I I I CONTROL I I I 600 mg NaCI/L ~ I I I 800 mg NaCI/L I I 1000 mg NaCI!L I I 1200 mg NaCI!L I 1400 mg NaCJIL I lage 106 of 107 -
I Page6of6 I Species: Ceriodaphnia dubia CdNaCLCR #: 8q I
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I CONTROL I I ~ 600 mg NaCIIL 800 mg NaCIIL 1000 mg NaCI/L 1200 mg NaCI/L 1400 mg NaCI!L 107 of 107
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Na~-~VA~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _E. Q_BOX..£.900 _ _ _ _ _ _ _ _ _ _ _ _
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NAME!TITLE PRINCIPAL EXECUTIVE OFFICER !I Cert1fy under penalty of law that th1s document and all attachments were prepared under my Timothy P. Cleary
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dI ! TELEPHONE DATE persons who manage the system, or those persons directly responsible for gathenng the Information, the information submitted 1s , to the best of my knowledge and belief, true, Prrncipal Environmental Engmeer I 423 843-6700 09 06 09 Sequoyah Site Vice President accurate, and complete. I am aware that there are s1gmficant penalties for submitting false r TYPED OR PRINTED information, including the possibility of fme and impnsonment for know1ng violations SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE I I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The Turbine Building Sump (TBS) was discharged directly to the Yard Pond (YP) on 5/5/09@ 0850- 5/9/09@ 1425 and on 5/11/09@ 1625- 5/11/09@ 1740. EPA Form (REV 3199) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include FaCilitY Name/Location 1f Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres_L_f~BOX..£900 - - - - - - - - - - - - TN0026450 107 G F- FINAL
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER l Cert1fy under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE Timothy P Cleary direction or supervision 1n accordance w1th a system des1gned to assure that qualified personnel properly gather and evaluate the information submitted. Based on my 1nquiry of the person or ~fudtwi<.LOc i persons who manage the system, or those persons dJrectly responsible for gathering the Principal Environmental Engineer Information, the information submitted JS , to the best of my knowledge and belief, true, 423 843-6700 09 06 09 Sequoyah Site Vice President accurate, and complete. l am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE L__ TYPED OR PRINTED Information, Including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE NUMBER YEAR MO I DAY-COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~VA-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Add res.§__£ ~BOX..lQOO _ _ _ _ _ _ _ _ _ _ _ _
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) [phW"'-'lL (}~Lei I Cert1fy under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the informalion submitted. Based on my inquiry of the person or I persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer II 423 843-6700 09 09 informat1on, the information subm1tted IS , to the best of my knowledge and belief, true, 06 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalt1es for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE I I
I TYPED OR PRINTED information, including the poss1bil1ty of fine and tmprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE l NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV'3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Na~-~VA-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _E ~BOX..£900 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 110 T F- FINAL ---~TEROFFICESB-2A-SQ~-------- - _ _ _§_ODD..'!:_- DAISY_ .....Jt:!.]738i_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facility_ ..JYA -~QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l._ _!:!AMIL TO.!::!..COUNTY __________ _ EFFLUENT ATTN Stephanie A. Howard From NO DISCHARGE IXX I *** NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUENCY! SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE ?DAY CHR ******** ******** ******** ******** 23 CERIODAPHNIA iTRP3B 0 0 ;cOMPOs 1 EFFLUENT GROSS VALUE IC25 STATRE ?DAY CHR PIMEPHALES TRP6C EFFLUENT GROSS VALUE 0 0 RE6~~~~~EN-r'Jr~ .)~~r SAMPLE I MEASUREMENT ~---*---~~-----*--~-* .., ,. J'
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)~on~ee_a.ifOUXu-d ! NAMEffiTLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direction or superv1sion in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or I persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 09 06 09 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false I Ir i__ TYPED OR PRINTED 1nformat1on, 1nc!uding the possibi!1ty of fine and impnsonment for knowing VIolations.
SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT I-AREA CODE l NUMBER YEAR MO I--- DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous ed1t1ons may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Add res.§_ ..J:.~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A-SQ~-------- TN0026450 116 G F- FINAL
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my dJrect1on or supervrs1on tn accordance w1t~ a syste~ des1gned to assu.re t~at qualtf1ed personnel
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d TELEPHONE Timothy p. Cleary properly gather and evaluate the 1nformat1on submitted. Based on my 1nqu1ry of the person or UC persons who manage the system, or those persons d~rectly responsible for gatherong the Principal Environmental Engineer
. . . IinformatiOn, the mtormat1on submJtted 1s , to the best of my knowledge and bel1ef, true, 423 843-6700 Sequoyah S1te ViCe President jaccurate. and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE '------ *Information, 1nclud1ng lhe poss1bility of f1ne and impnsonment for know1ng VIOlations. OFFICER OR AUTHORIZED AGENT AREA TYPED OR PRINTED 1 CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres_L ___.!: Q_BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A-SQ~--------
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER II Certify under penalty of law that this document and all attachments were prepared under my Timothy P. Cleary Idirection or supervis1on in accordance with a system d. esigned to assure that quali.fied personnel properly gather and evaluate the 1nformat1on submitted Based on my inqu1ry of the person or hOJvi. (). ev--cl TELEPHONE persons who manage the system, or those persons d1rect!y responsible for gathenng the Principal Environmental Engineer
. . . '[Information. the informat1on subm1tted is. to the best of my knowledge and belief, true, 423 843-6700 06 09 Sequoyah S1te V1ce President ,accurate. and complete I am aware that there are S1gn1f1cant penalt1es for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE
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COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~VA~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres.L_ _f.~BOX.1.QOO _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A-SQ~-------- TN0026450 118 G F - FINAL - _ _ _§_ODD_'!.: DAISY_ _IN 3738+/-_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER I WASTEWATER & STORM WATER Factlitv _ _IYA -_.§_EOUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.Q_ .J:!AMIL TO.t!._COUNTY __________ _ MONITORlli.G_E'ERIOD EFFLUENT YEAR-, DAY NO DISCHARGE I XX I ... ATTN Stephanie A Howard From 09 To 31 NOTE: Read instructions before completinq this form. PARAMETER QUALITY OR CONCENTRATION I NO. \FREQUENCY\ SAMPLE TYPE UNITS MINIMUM AVERAGE MAXIMUM UNITS EN, DISSOLVED (DO) ******** ******** 19 0 0
.4 LIDS, TOTAL SUSPENDED 0 0 SOLIDS, SETTLEABLE 00545 1 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 0 0
~ tfphrv~U ~o----d NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance w1th a system designed to assure that qualified personnel
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Timothy P. Cleary properly gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system. or those persons directly responsrble for gathering the Principal Environmental Engineer 1nformat1on, the 1nformat1on subm1tted IS , to the best of my knowledge and belief, true, 423 843-6700 09 9 Sequoyah Site Vice President accurate, and complete I am aware that there are significant penalties for subm1ttmg false SIGNATURE OF PRINCIPAL EXECUTIVE '
--~ _____ ---~----*-------- ______ . ______ ------* _____ _jtnformatlon. 1ncludtng the poss1btl1ty of f1ne and 1mpnsonment for knowing violations TYPED OR PRINTED I OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR M COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
S58 090714 800- NPDES CORRESPONDENCE July 14, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 40 1 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JUNE 2009 Enclosed is the June 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of the Sequoyah Environmental staff.
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Tim::::lleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN G. R. Signer, WT 6A-K T. P. Cleary, OPS 4A-SQN B. A. Wetzel, OPS 4A-SQN R. M. Payne, LP 4B-C EDMS, WT CA-K (Enclosure) DMR0906.doc
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JUNE 2009 ORGANIZATION: Chemistry and Environmental DOCUMENT PREPARED BY: Ann Hurt DATE: 07/09/2009 CATEGORY: NPDES Correspondence CONCURRENCES Name R c Signature - Comment Date v N Ann Hurt X Stephanie A Howard X Alan K Barringer X Debra J Bodine X Beth A. Wetzel X Chris R Church X Timothy P Cleary X ~-- (for signature) 4) 1Lt --1 u u::P( INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
Tennessee Valley Authority Post Office Box 2000 Soddy Daisy, Tennessee 37384-2000 Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant July 14, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JUNE 2009 Enclosed is the June 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at ( 423) 843-6714 or Stephanie Howard at ( 423) 843-6700 of the Sequoyah Environmental staff. ~Jp~
- rn:h~J. Cleary Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATIN: Document Control Desk Washington, D.C. 20555 pnnted on recycled paper
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ Form Approved MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No 2040-0004 (SUBR 01) Address P.O. BOX 2000
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Timothy P. Cleary properly gather and evaluate the informat1on submttted Based on my 1nquiry of the person or persons who manage the system, or those persons d1rectly respons1ble for gathering the Principal Environmental Engineer 1nformat1on, the informatmn subm1tted 1s , to the best of my knowledge and beltef, true, 423 843-6700 1 091 07 r 4 I L_ Sequoyah Site Vice President TYPED OR PRINTED accurate, and complete. I am aware that there are S1gn1f1cant penalties for submitting false informat1on, including the possibility of f1ne and 1mpnsonment for knowing violat1ons I SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE
---NUMBER- 'YEAR~
I F COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2
DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 06/10/2009 @ 1112 0.16 mg/1 06/15/2009 @ 1112 JOB EPH CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 06/10/2009 @ 1110 <0.10 mg/1 06/15/2009 @ 1123 JOB EPH
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEARPLANT ___ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _.E. ~80l<_1900 _ _ _ _ _ _ _ _ _ _ _ _
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Principal Environmental Engineer I I information, the information submitted is , to the best of my knowledge and belief, true. 423 843-6700 09 07 I 14 I Sequoyah Site Vice President accurate, and complete. I am aware that there are sign1f1cant penalt1es for submitting false r--siGNATURE OF PRINCIPAL EXECUTIVE IDAY] informat1on, 1ncludmg the poss1bility of fme and Imprisonment for know1ng violations I TYPED OR PRINTED I OFFICER OR AUTHORIZED AGENT AREA I NUMBER IYEAR I MO I __ CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured: 1. Biodetergent 73551 (max. calc. cone. was 0.019mg/L--Iimit 2.0mg/L) 2. MSW-1 01 (max. calc. cone. was 0.077mg/L-Iimit 0.2mg/L) 3. H-150M (max. calc. cone. was 0.039mg/L--Iimii 0.050mg/L) 4. H-150M (low detection level analytical method was <0.050mg/L--Iimit 0.050mg/L) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 2 of 2
PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) AddresL_E~BOX2000 - - - - - - - - - - - -
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I i 09~1' i persons who manage the system, or those persons directly responsible for gathering the I Information, the information submitted is , to the best of my knowledge and belief. true, Principal Environmental Engineer 423 843-6700 Sequoyah Site Vice President -- I c - TYPED OR PRINTED
------** L accurate, and complete. I am aware that there are Significant penalties for submitting false information, including the possibility of f1ne and impnsonment for knowing violations SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT -j-AREA CODE NUMBER \YEAR- MO DAY_
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Boron was sampled on 04/08/2009. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT IDMRJ OMB No. 2040-0004 (SUBR 01) Add res.§_ __E_.~BOX.AJOO _ _ _ _ _ _ _ _ _ _ _ _ F- FINAL
---~TEROFFICESB-2A-SQ~-------- - _ _ __§_ODD.Y_- DAISY_ _}t!]738i__ _ _ _ _ _ _ _ BIOMONITORING FOR OUTFALL 101 Facility_ JYA -_§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _
Locatio.!l_ _!:!AMIL TO.Ji_COUNTY EFFLUENT ATTN: Stephanie A. Howard NO DISCHARGE CJ *** NOTE: Read instructions before completinq this form PARAMETER QUANTITY OR LOADING
*-----co::-:u-A._L_I_TY---=o-=R:-c-::-o~N-=c-=E-N~TRA T-ION I NO. 1 FREOUEN_c_Y_S_A_M_P_L_E_
EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS I ANALYSIS IC25 STATRE 7DAY CHR ******** ******** ******** ******** 23 CERIODAPHNIA TRP3B 0 0 SEE" rCOMPOS EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES I,TRP6C 1 0 0 SEE .. ICOMPOS,I
!EFFLUENT GROSS VALUE I .
NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my direction or supervision 1n accordance Wllh a system des1gned to assure that qual1f1ed personnel .'*' '
/Jr.tphcut,.u: . .C'J,\k~ll~~c.:...zcl i \, I 1
TELEPH~ DATE j I o~-:-T,~ Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inqwy of the person or persons who manage the system, or those persons d1rectly responsible for gathenng the Principal Environmental Engineer
. . . 1nformatton. the 1nformat1on submttted is , to the best of my knowledge and bel1ef, true, 423---:3-6700 1 AREA~UMBER Sequoyah S1te VIce President accurate, and complete 1am aware that there are sigmf1cant penalties for subm1tt1ng false SIGNATURE OF PRINCIPAL EXECUTIVE kEAFJ MO iDAY_1.
_ _ _ _ _ _ _ __,1information, 1ncluding the possibt!1ty of f1ne and tmpnsonment for know1ng violat1ons OFFICER OR AUTHORIZED AGENT l____ TYPED OR PRINTED _l_ CODE l_ *- ~o COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in June 2009. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ Add res§_ ...£..9c._BOX.1.QOO _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A-SQ~--------
- _ _ 20DD.1_- DAISY _ _IN 3738'!_ _ _ _ _ _ _ _
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ATIN: Stephanie A. Howard NOTE: Read instructions before completinq this form. r PARAMETER QUANTITY OR LOADING -----,----------=a=-cuc-:A-:-Lc-.:IT~Y-=oc=Rc-:C:c:O::-:cN-:-:C:-::E::-:N=TR=-A::-:T:::-IO::cN-:--------------rNo.vREOUENCTSAMPLE I I EX I OF TYPE 1--- AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS I IPH SAMPLE ******** ******** ** 7.1 ******** I 89 12 0 13 I 30 GRAB I ! MEASUREMENT I . I ~~~~0L0uEN~ f-- 0 0 GR oss vALUE
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'I TELEPHONE=f DATE~~
Timothy P. Cleary properly gather and evaluate the 1nforma!ion submitted Based on my InqUiry of the person or ,.>LJ,*"'-4; ¥Lu... v *'- * - I persons who manage the system or those persons d. ~rectly respons1ble for gathenng the PrinCipal Environmental Engmeer I oj**** . . 1 14 1'
. . . 1nformat1on the Information subm1tted 1s to the best of my knowledge and bel1ef true ~ =bi423 843-6700 09 07 1 Sequoyah Site VICe President accurate. and complete I am aware that there are 51gnif1cant penalties for submitt*ng false SIGNATURE OF PRINCIPAL EXECUTIVE _1__ 1 , 1 1 - .-
1
- - - - - - - - * - - - - - ------1'nformat1on. 1nclud1ng the possibility of fine and 1mpnsonment for know1ng violat1ons. OFFICER OR AUTHORIZED AGENT AREA 1 NUMBER YEAR I' MO DAY j
- l *-*-~* ----
TYPED OR PRINTED ... _ _ _ _ _ .. CODE I I _j COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facililv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) MAJOR Form Approved. Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT !DMRJ OMB No. 2040-0004 (SUBR 01) Addres.L_ _E ~BOX_?.900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A-SQ~-------- TN0026450 I ~~ 107 G F- FINAL - _ _ _J?_ODDY- DAISY_ _IN 3738~ _ _ _ _ _ _ _ Facility_ .JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ _F=1'BMLlJ'!ldiVI__ill:~ ~ARGE NUMBER METAL CLEANING WASTE POND Locatio.!}_ _!:!AMIL TOJ::!.COUNTY._ _ _ _ _ _ _ _ _ _ _ c= [iEARJ MP MONITORING PERIOD "T DAY I Cii:AAI"'""--~~~~D~~~y~ EFFLUENT
*** NO DISCHARGE @] ***
ATTN: Stephanie A Howard From Cos I o6I o1l To [ os-:T os I 30] NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. FRE.--:Q_U_E_N-:-CY---r-:-S_A_M_P_L_E---, EX OF TYPE
~I AVERAGE MAXI~~---- UNITS MINIMUM AVERAGE ------;.;,-AX~MUM UNITS ANALYSIS I
[PH- SAMPLE MEASUREMENT I 12 I 00400 1 0 0 PE,RMiT ***" '******** s.O{** . - .* ........ *:*~ * . _I . . 9.0 :; su .DAILY .!**GRAB EFFLUENT GROSS VALUE RECiUIJ:{EMENI
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- REPORT .
. DAit.Y l\llx MGD I ******** ~j ""*"*11 ..... ; l ******** II . I DAILY lpALCTDl NAME!TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that.thls doc.u ment and all attachments were prepared un er my
*drrect 1on or superv1s1on 1n accordance wrth a system desrgned to assure that qualrfred ersonnel I *: '-....
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. Timothy p. Cleary properly gather and evaluate the 1nformat1on subm1tted. Based on my rnqu1ry of the per on or I l I 07 1 I persons who manage the system. or those persons d11ectly respons1ble for gathenng th Principal Environmental Engineer 14 1 ! . . . rnformatron. the informatron submitted rs . to the best of my knowledge and belief. true. 423 843 - 6700 09 ! ! Sequoyah Stte V1ce President accurate. and complete. 1am aware that there are s1gn1ficant penaltresfor subm1tling fa se SIGNATURE OF PRINCIPAL EXECUTIVE I ' ~
f.-- **---- Information. Including the poss1b111ty of frne and rmpnsonment for knowrng vrolatrons OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR I MO [DAY L__ TYPED OR PRINTED CODE _ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Na~-~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L ___E.~BOX.1_900 ___________ _ F- FINAL
---~TEROFFICESB-2A-SQ~-------- - _ _ _§ODD..Y_- DAISY_ _IN 37381_ _ _ _ _ _ _ _ RECYCLED COOLING WATER Facilitv_ _IVA -..§EQUOYAH NUCLEAR PLANT_ _ _ _ _ _
Locatio.!!_ J::!AMIL TOJi.COUNTY __________ _ EFFLUENT NO DISCHARGE I XX I ... ATTN: Stephanie A. Howard NOTE: Read instructions before completinq this form. IFREQUENCY
~
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******** ******** ******** 12 i
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!CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** ********
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- l I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER j
_,Jil.PhCUt)~tJ.\(tk~l{~:tttc\ I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or superv1sion in accordance w1th a system designed to assure that qualified personnel I properly gather and evaluate the informat1on submitted. Based on my inquiry of the person or Timothy P. Cleary i persons who manage the system, or those persons d1rect!y responsible for gathenng the Principal Environmental Engineer information, the tnformation subm1tted 1s , to the best of my knowledge and bel1ef, true, 423 843-6700 09 I 07 14 I Sequoyah Site Vice President accurate, and complete. I am aware that there are s1gn1ficant penalt1es for subm1tt1ng false information, tncludmg the possibility of ftne and 1mpnsonment for know~ng vioiatJOns SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT ~--~- AREA DAY NUMBER YEAR I MO TYPED OR PRINTED I CODE ! L I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Penod EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres.§_.£..~BOX_1900 - - - - - - - - - - - - F- FINAL
---~TEROFFICESB-2A-SQ~--------
_ _ _ ..§.ODD...::L- DAISY_ _IN 37381._ _ _ _ _ _ _ _ RECYCLED COOLING WATER Facilitv_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio!!_ __!:!AMIL TO.!!_COUNTY __________ _ EFFLUENT NO DISCHARGE li5_~ *** ATTN: Stephanie A. Howard
~ NOTE: Read instructions before completinq this form.
I PARAMETER x* QUANTITY OR LOADING - QUALITY OR CONCENTRATION NO. EX FREQUENCY OF SAMPLE TYPE l I I AVERAGE I MAXIMUM I UNITS MINIMUM I AVERAGE I MAXIMUM I UNITS ANALYSIS IIC25 STATRE 7DAY CHR CERIODAPHNIA .. c SAMPLE A " ' !DC . . C. IT
******** I ******** I ** I I ******** I ******** I 23 I ---l TRP3B 1 0 0 !EFFLUENT GROSS VALUE ilc25 STATRE 7DAY CHR JPIMEPHALES TRP6C 1 0 0 IEFFLUENT GROSS VALUE h-II ~d~ 1
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\..A... ~ *~- "' I I I 09 Sequoyah Site V1ce President persons who manage the system or those persons d~rectly responsible for gathenng the tnformatton the 1nformat1on subm1tted 1s to the best of my knowledge and belief true accurate, and complete 1am aware that there are stgn*ficant penalties for submttttng false Information, tnciud1ng the possib1l1ty of fine and 1mpnsonment for know1ng VIOlations J Pnnc 1pal Environmental Engmeer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 1
~
423
----*ry--
843-6700 NUMBER YEAR I I
~
07 MO it' 14 1 DAY 1 J _____TY __ ~EE_OR PRINTED _ _ _ _ _ L _ _ _ ____j__~ _ I __j COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editiOns may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres_L ___f.~BOX~OO ___________ _ ---~TEROFFICESB-2A-SQ~-------- TN0026450 I L_ 116 G J F- FINAL - _ _ __§_ODDY- DAISY_ _IN 3738~ _ _ _ _ _ _ _ C:RMIT NUMBER ] [DISCHARGE NU~ BACKWASH Facility_ _sJA- ~QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatlo.!l_ _!:!AMIL TOJ:!..COUNTY __________ _ MONITQRLNG PERIOD ] EFFLUENT
~l LiEAR I MO I D_6i]
ATTN: Stephanie A. Howard To [~] 06 I 30 l NO DISCHARGE CJ *** NOTE: Read instructions before completinq this form I PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NU. EX
!FREQUENCY!
OF SAMPLE-~ TYPE I AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS ____j JoEBRIS ******** ******** ******** ******** 0 0 1 I 30 VISUAL 9A I 101345 1 0 0 REPORF:. PASS=O 'VISUAL ,EFFLUENT GROSS VALUE MO'lOTAl.* FAIL=1 OIL AND GREASE VISUAL ******** 84066 1 0 0 SEE ! VISUAL ~EFFLUENT GROSS VALUE PERMit ~-;:;~:~;; 1
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' I . I I I I I I I NAMErTITLE PRINCIPAL EXECUTIVE OFFICER
/~--k~~;~lli.Jt.-c~C~-tlo'1~0-zcl I Certify under penalty of !aw that this document and all attachments were prepared under my TELEPHONE DATE direct1on or supervision in accordance with a system designed to assure that qualified personnel 09~o;~
! Timothy P. Cleary properly gather and evaluate the informat1on submitted. Based on my inquiry of the person or I
persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 information, the information subm1tted 1s , to the best of my knowledge and bel1ef, true. YEARrMoJ~AY Sequoyah Site Vice President Iaccurate, and complete. I am aw~re that there are s1.gn1ficant penalties. for subm1ttmg false I SIGNATURE OF PRINCIPAL EXECUTIVE I 1nformat1on, 1nc!udmg the poss1b111ty of fine and 1mpnsonment for know1ng VIOlations I TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT 1---;:;:REA-t- NUMBER L _ __ _ _ _ _ _ _ _ - * - - - - - - 1 CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) AddresL _E ~BOX.AJOO _ _ _ _ _ _ _ _ _ _ _ _
---~TEROFFICESB-2A-SQ~-------- l -- TN002~~--J I 117 JLJ F- FINAL - _ _ _!2_0DD_l_- DAISY_ ___JN 3738'!.._ _ _ _ _ _ _ _ [ -pffiMiTNUMBER-] [DISCHARGE NU~ BACKWASH Facility _ _IYA -..§!::QUOYAH NUCLEAR PLANT_ _ _ _ _ _
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I I I SAMPLE MEASUREMENT I PEHMIJl' ,: ; BJ;aliiiREMENT . I I I L -~-~ I NAMEoffiTLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this document and all attachments were prepared under my direction or superviSIOn 1n accordance w1th a system des1gned to assure that qual1f1ed personnel Jtivt _*
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G\--!\ <tk~;fl,.L(.I.-l(_ TELEPHONE I DATE 1 Timothy P. Cleary Iproperly gather and evaluate the 1nformalion subm1tted. Based on my 1nquiry of the person or / ' - I I ' ji;Rr::t~A~ persons who manage the system, or those persons directly respons1ble for gathenng the Principal Environmental Engineer rI Sequoyah S1te Vice PreSident 1nformat1on, the 1nformat1on submitted 1s, to the best of my knowled~e and bel1ef, true, accurate, and complete. 1am aware that there are sigmf1cant penalt1es for subm1tt1ng false i--::- SIGNATURE OF PRINCIPAL EXECUTIVE 423 I 843-6700 I . --~~--~Information, including the poss1b111ty of fine and imprisonment for know1ng violations I OFFICER OR AUTHORIZED AGENT AREA I NUMBER L TYPED OR PRINTED : CODE _ _j_______ -~~ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres_L ...£_~BOX 20QQ_ _ _ _ _ _ _ _ _ _ _ _ _
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ATTN: Stephanie A. Howard o9 I o6 I o_i] Tol 09 NOTE: Read instructions before completinq this form. I PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREOUENCY-.-5-A_M_P_L~ EX OF TYPE I -AVERAGE- -MAXIMUM MINIMUM 11\/E.RAGE MAXIMUM UNITS ANALYSIS Ia: SAMPLE MEASUREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this documenl and all attachments were prepared under my wit~ a system d. es1gned to assure t~at qualified personnel r:* J.;l-,} f'~t'}/',, 'i{ ; ( I ' ' c*.. i\ j'.: f 1 \ f i/;./ 'l '\j', f r-\. TELEPHONE I DATE
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direct 1on or supervision 1.n accordance ! Timothy P Cleary properly gather and evaluate the 1nformat10n subm1tted. Based on my 1nqu1ry of the person or /"<*fll4JY [i.},...V _.._, --\./\ --~ \! ..-'v~- ~ 1 1 persons who manage the system, or those persons drrectly responsible for gatherrng the Principal Environmental Engineer 1 i 1nformatron. the rnformat1on submitted 1s, to the best of my knowledge and belle( true. . 4 23 843-670 0 . 09 07 . 14
! Sequoyah S1te V1ce President Iaccurate, and complete. 1am aware that there are significant penalt1es for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I__.. I ~ ---j1nformat1on, rncludrng the poss1brlrty of frne and rmprrsonment for know1ng V1olat1ons OFFICER OR. AUTHORIZED AGENT _j' AREA NUMBER 1 YEAR MO I DAY 1 I TYPED OR PRINTED ___L---~~------------ _CODE --~_____L_ _ l_ _j COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all ;ttachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1}}