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1.e.iii.2.9, Sequoyah Nuclear Plant (Sqn) Annual Water Withdrawal Updates for 2012, NPDES Permit No. TN0026450-Discharge Monitoring Report (DMR) for July 2012
	ML13289A202
Person / Time
Site:	Sequoyah
Issue date:	08/15/2012
From:	Simmons P R; Tennessee Valley Authority
To:	Morgan C; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation
Shared Package
ML13289A109	List: ML13289A092; ML13289A094; ML13289A095; ML13289A096; ML13289A097; ML13289A098; ML13289A099; ML13289A100; ML13289A101; ML13289A108; ML13289A111; ML13289A112; ML13289A113; ML13289A114; ML13289A115; ML13289A116; ML13289A117; ML13289A118; ML13289A119; ML13289A120; ML13289A121; ML13289A122; ML13289A123; ML13289A124; ML13289A125; ML13289A126; ML13289A127; ML13289A128; ML13289A129; ML13289A130; ML13289A132; ML13289A133; ML13289A134; ML13289A136; ML13289A137; ML13289A153; ML13289A158; ML13289A164; ML13289A165; ML13289A166; ML13289A167; ML13289A168; ML13289A169; ML13289A170; ML13289A171; ML13289A172; ML13289A173; ML13289A174; ML13289A197; ML13289A198; ... further results
References
	Download: ML13289A202 (164)
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{{#Wiki_filter:S58 120815 800-NPDES CORRESPONDENCE August15,2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450 -DISCHARGE MONITORING REPORT (DMR) FOR JULY 2012 Enclosed is the July 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. During July, natural conditions caused the 24-hour average ambient river temperature to exceed 29.4°C for 18 days, including 7/29 and 7/30 when the lnstream Monitoring temperature measured 30.6°C. The plant continued to operate in helper mode throughout this period and complied with the elevated 1-hour average river temperature limit of 33.9oc at the downstream edge of the mixing zone as required by Part 1.A.1.f of the NPDES permit. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. a:t Paul R. Simmons Plant Manager Signatory Authority for: John T. Carlin Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D. Booker, MOB 1 F-WBN B. E. Brickhouse, BR 4A-C J. T. Carlin, OPS 4A-SQN J. A. Cross, POB 2A-SQN T.R. Mgrkum, BR 4A-C D. B. Nida, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 J.W. Proffitt, OPS 4C-SQN A. A. Ray, WT 11 A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1 E-M Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 August15,2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450 -DISCHARGE MONITORING REPORT (DMR) FOR JULY 2012 Enclosed is the July 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. During July, natural conditions caused the 24-hour average ambient river temperature to exceed 29.4°C for 18 days, including 7/29 and 7/30 when the lnstream Monitoring temperature measured 30.6°C. The plant continued to operate in helper mode throughout this period and complied with the elevated 1-hour average river temperature limit of 33.9oC at the downstream edge of the mixing zone as required by Part 1.A.1.f of the NPDES permit. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Signatory Authority for: John T. Carlin Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} ___ _ ___________ _ Faqlli!y_ JYA-SEQUOYAH NUCLEAR PLANT __ ...;_ __ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 101 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE MONITORING PERIOD EFFLUENT From DAY I I YEAR I MO I DAY *** NO DISCHARGE D*** Form Approved. OMB No. 2040-0004 A TIN: Brad Love 12 I 07 I 01 I To I 12 I 07 I 31 NOTE: Read instructions completin!i this fonn. PARAMETER WATERDEG. CONDUIT OR THRU ENT PLANT 1 0 GROSS NAMEITITLE PRINCIPAL EXECUTIVE OFFICER Paul R. Simmons QUANTITY OR LOADING I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personne properly gather and evaluate the information submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the QUALITY OR CONCENTRATION NO. SAMPLE EX TYPE DATE information, the information submitted is , to the best of my knowledge and belief, true, accurate, 1------------------l Sequoyah Plant Manager and complete. 1 am aware that there are significant penalties for submitting false Information, 423 843-6502 12 08 15 1------------------lincluding the possibility of fine and Imprisonment for knowlng violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference afl attachments here} SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE NUMBER YEAR MO DAY No closed mode operation. Veliger monitoring data is included as an attachment. Natural conditions caused the 24-hour average ambient river temperature to exceed 29.4"C for 18 days, including 7/29 and 7/30 when the lnstream Monitoring temperature measured 30.6"C. The plant continued to operate in helper mode throughout this period as required by Part 1.A.1.f of the NPDES permit. EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 Mean# of Water Mean# of Water NOTES:% Sample Date ZM/m3 %Settlers Temp. fC) Sample Date Asiatic Temp.("C) LOCATION SUB LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 PB 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 PB 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May 2012 No Samples Collected June 2012 No Samples Collected July 2012 No Samples Collected PERMITTEE NAME/ADDRESS (Include Facility NameA..ocation if Different) ___ _ AddresL_f.Q,_BOX2000 _______ _ ____ _ ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT {DMRJ TN0026450 PERMIT NUMBER MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 BIOMONITORING FOR OUTFALL 101 EFFLUENT

- - NO DISCHARGE D *** NOTE: Read instructions before this fonn. PARAMETER QUALITY OR CONCENTRATION NO. SAMPLE NAME/TITLE PRINCIPAl EXECUTIVE OFFICER Paul R. Simmons ********

I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, 1-----------------1 Sequoyah Plant Manager and complete. 1 am aware that there are significant penalties for submiHing false information, 1------------------!including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY (Reference all attachments here) Toxicity was not sampled in July 2012. EPA Fonn 3320-1 (REV 3/99) Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT EX TYPE TELEPHONE DATE 423 843-6502 12 08 15 AREA NUMBER MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location

r Different)

___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM {NPDESJ DISCHARGE MONITORING REPORT {DMRJ MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Address_...f..Q,_BOX2000 TN0026450 PERMIT NUMBER ____ _ A TIN: Brad Love PARAMETER 0 DUITORTHRU ITI:III:::AT

- CI..IT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER Paul R. Simmons Sequoyah Plant Manager TYPED OR PRINTED From 12 QUANTITY OR LOADING I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the infonnation submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the infonnalion, the infonnation submitted is , to lhe best of my knowledge and belief, true, aa:urate, and complete. I am aware that there are significant penalties for submitting false infonnation, including the possibility of fine and imprisonment for knowing violations. COMMENTS AND OF ANY (Reference aH attachments here) EPA Form 3320-1 (REV 3199) Previous editions may be used 103 G 31 LOW VOL. WASTE TREATMENT POND EFFLUENT '*** NO DISCHARGE D '*** NOTE: Read instructions before completinn this form. QUALITY OR CONCENTRATION NO. SAMPLE EX TYPE -A 6F-?fr ..IELEPHONE DATE Plant Manager 423 843-6502 12 08 15 SIGNATURE OF PRINCIPAL EXECUTIVE 1 OFFICER OR AUTHORIZED AGENT AREA I NUMBER MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} ___ Addres.!__g.o. BOX_6900 _______ _ FaQ!i!y_ J)/A-SEQUOYAH NUCLEAR PLANT _____ _ ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ TN0026450 MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE I XX I *** Form Approved.

OMB No. 2040-0004 NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. SAMPLE WATERDEG. -C, RATE OF NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER Paul R. Simmons ****--1 Certify under penalty of law that this document and all attachments were prepared under my direction ar supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submiHed. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the ** __ _ Sequoyah Plant Manager information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submiHing false information, 1-------------------lincluding the possibility affine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Fonn 3320-1 (REV 3/99) Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 AREA CODE EX TYPE DATE 843-6502 12 08 15 NUMBER YEAR MO DAY Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) __ Address _ _e.o. BOX20QQ.. ___________ _ _______ _ ____ _ ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ TN0026450 110 T PERMIT NUMBER DAY 31 MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE I XX I *** Form Approved.

OMB No. 2040-0004 NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 0 0 EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 0 EFFLUENT GROSS VALUE SAMPLE 'ol PERMIT' ,) '

SAMPLE F , REQUIRE:ME:NT

. ::, ';_: SAMPlE AVERAGE MAXIMUM UNITS MINIMUM **-**** -NAMEITITLE PRINCIPAL EXECUTIVE OFFICER Paul R. Simmons I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submiHed. Based on my inquiry of lhe person or persons who manage lhe system, or those persons directly responsible for gathering lhe AVERAGE MAXIMUM ********

- -**-Sequoyah Plant Manager information, the Information submitted is. to the best o1 my knowledge and belief, true, accurate, 1------------------1 and complete.

I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAl EXECUTIVE 1------------------lincluding the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY (Reference all attachments here) No Discharge this Period EPA Fonn 3320-1 (REV 3199) Previous editions may be used UNITS EX OF TYPE ANALYSIS 23 'tfSEf.AI*** ' CQM!'QS *>-* TElEPHONE DATE 423 843-6502 12 08 15 AREA NUMBER MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location ff Different} ___ _ _____ ____ _ ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR} TN0026450 PERMIT NUMBER YEAR MAJOR (SUBR 01) F-FINAL WASTEWATER & STORM WATER EFFLUENT -NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. SAMPLE EFFLUENT GROSS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Paul R. Simmons AVERAGE MAXIMUM ******** I Certify under penalty of law that this document and all attachments were prepared under my direction or supeJVision in accordance with a system designed to assure that qualified personn properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, t------------------1 Sequoyah Plant Manager and complete. 1 am aware that there are significant penalties for submiHing false information, 1-------------------tincluding the possibility of fine ancl imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Prewous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT EX TYPE TELEPHONE DATE 423 843-6502 12 08 15 AREA NUMBER MO DAY CODE Page 1 of 1 REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-July 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 8/03/2012 CONCURRENCES Name R c Signature -Comment Date v N B.M. Love X L.M. Koby X J.A. Cross X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended

purpose, logical in construction, and clear in meaning in the eyes of the recipient.

A concurrence signature indicates that the individual would be willing to sign the document for the agency. S58 120910 800-NPDES CORRESPONDENCE September 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville , Tennessee 37219

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-DISCHARGE MONITORING REPORT (DMR) FOR AUGUST 2012 Enclosed is the August 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. If you have any questions or need additional information , please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted . Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information , the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information , including the possibility of fine and imprisonment for knowing violations . Seq't:teyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga , Tennessee 37 402-2013 S.D. Booker, MOB 1 F-WBN B. E. Brickhouse, BR 4A-C J. T. Carlin, OPS 4A-SQN J. A. Cross, POB 2A-SQN T.R. Markum, BR 4A-C D. B. Nida, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 J.W. Proffitt, OPS 4C-SQN A. A. Ray, WT 11 A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1 E-M Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 September 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville,Tennessee 37219

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-DISCHARGE MONITORING REPORT (DMR) FOR AUGUST 2012 Enclosed is the August 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. -.......""""'-'T=':* e President Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 REVIEW /CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-August 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 9/06/2012 Name B.M. Love L.M. Koby J.A. Cross R C V N X X CONCURRENCES Signature -Comment Date INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and

indicate agreement with commitments made which are applicable to the revieWer's organization.

CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended

purpose, logical in construction, and clear in meaning in the eyes of the recipient.

A concurrence signature indicates that the individual would be willing to sign the document for the agency. PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Address P.O. BOX 2000 _______ _ Facility TVA-SEQUOYAH NUCLEAR PLANT HAMIUONCOUNrr========== A TIN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMR) TN0026450 PERMIT NUMBER 101 G DISCHARGE NUMBER DAY 31 MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT

- - NO DISCHARGE D ... Form Approved.

OMB No. 2040-0004 MO e;:wr MO 08 I 01 J Tor 12 I 08 NOTE* Read instructions before this form PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ********

42.4 0 31/31 RCORDR ** 04 CENTIGRADE MEASUREMENT 00010 1 0 PERMIT ********

- - - ********

Req. Mon. DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS ----* TEMPERATURE, WATER DEG. SAMPLE ********

30.4 0 31/31 MODELD ** 04 CENTIGRADE MEASUREMENT 00010 z 0 PERMIT ********

- - - ********

30.5 DEG.C. CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAILYMX NUOUS ------

- - - - -

---TEMP. DIFF. BETWEEN SAMP. & SAMPLE ********

2 0 31/31 CALCTD ** 04 UPSTRM DEG.C MEASUREMENT 00016 1 s PERMIT ********

- - - ********

3 DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILYMX NUOUS *------------ --FLOW, IN CONDUIT OR THRU SAMPLE ******** 1770 ********

0 31/31 RCORDR 03 ** TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ********

.... CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS ---------- 1----------** ,--CHLORINE, TOTAL RESIDUAL SAMPLE ********

0.019 0.045 0 25/31 GRAB ** 19 MEASUREMENT 50060 1 0 PERMIT ********

- - - ********

0.1 0.1 MG/L FIVE PER CALCTD EFFLUENT GROSS REQUIREMENT MOAVG DAILY MAX WEEK ---******** CALCTD TEMPERATURE-C, RATE OF SAMPLE ******** 0 ******** 0 31/31 62 ** CHANGE MEASUREMENT 82234 1 0 PERMIT ******** 2 DEG ********

- - - - .... CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILYMX C/HR NUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT

__________ L.._ ____________ ,.,, NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my L:f\ a{fJM. TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel( John T. Carlin property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the "" \.:7 _.. rgireJe'e President Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 12 and complete. I am aware that there are significant penalties for submitting false information, Si(;f'fA"'rURE OF PRINCIPAL EXECUTIVE I including the possibility offine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR TYPED OR PRINTED CODE --COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred:

1. Floguard MS6236 (max. calc. cone. was 0.032mg/L--Iimit 0.2mg/L)

2. Biodetergent 73551 (max. calc. cone. was 0.029mg/L--Iimit 2.0mg/L)

3. Spectrus CT1300 (max. calc. cone. was 0.037mg/L--Iimit 0.050mg/L)

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 09 10 MO DAY Mean# of Water Mean# of Water NOTES:% Sample Date ZM/m3 %Settlers Temp.("C) Sample Date Asiatic Temp. ("C) LOCATION SUB LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV 1234 WBE 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE 04/03/2012 229 0 18 04/03/2012 0 . 18 1-ISV-24-1234 PB 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 PB 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May 2012 No Samples Collected June 2012 No Samples Collected July 2012 No Samples Collected August 2012 No Samples Collected PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres.!...__E.O. BOX2000 -__ .J!.NTEROFFICE OPS-5N-5QN)_

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 T MAJOR (SUBR 01) F-FINAL Form Approved.

OMB No. 2040-0004 SODDY-DAISY. TN 37384

=

PERMIT NUMBER DISCHARGE NUMBER I f0NIT1RING I BIOMONITORING FOR OUTFALL 101 EFFLUENT ATTN: Brad Love MO DAY YEA MO DAY From 12 I 08 01 To [ 12 08 I 31 *** NO DISCHARGE D ... NOTE: Read instructions before this form. PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE ?DAY CHR SAMPLE ********

Other ********

- 23 CERIODAPHNIA MEASUREMENT TRP3B 1 0 PERMIT I ********
  - - 43.2 ********

PERCENT SEMI COMPOS EFFLUENT GROSS MINIMUM ANNUAL ----*-*------- IC25 STATRE ?DAY CHR SAMPLE ********

Other ********

- 23 PIMEPHALES MEASUREMENT TRP6C 1 0 PERMIT ********
  - - 43.2 ******** ********

PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MIMINUM ANNUAL '-* ---** -----* SAMPLE PERMIT REQUIREMENT -**-SAMPLE PERMIT REQUIREMENT

1------------- -SAMPLE PERMIT REQUIREMENT --SAMPLE MEASUREMENT PERMIT REQUIREMENT

1---SAMPLE MEASUREMENT PERMIT REQUIREMENT

- -****----*

--/ '/'l/7 / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel .* ' John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or 'J 'fd bresident persons who manage the system, or those persons directly responsible for gathering the 423 843-7001 12 09 10 Sequoyah Site Vice President information. the information submitted is . to the best of my knowledge and belief, true. accurate. and complete. I am aware that there are significant penalties for submitting false information, OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. Ef-ICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled August 12-17. A revised August DMR including toxicity results will be submitted during the next reporting period. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Addres.§_ __f_.O. BOX 2000 ___________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 103 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 (INTEROFFICE OPS-SN-SQN) PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND EFFLUENT ATTN: Brad love *** NO DISCHARGE D ... NOTE: Read Instructions before completln!l this form. PARAMETER >< QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ********

7 ******** 8 0 14/31 GRAB ** 12 -00400 1 0 PERMIT ********

l ** 6 ******** 9 su THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK -***---*** -SOLIDS, TOTAL SUSPENDED SAMPLE ********

7 9 0 2/31 GRAB ** 19 00530 1 0 PERMIT ********

- ********

30 100 MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH *--------

-f-------*---*
-

OIL AND GREASE SAMPLE ********

<5 <6 0 2/31 GRAB ** 19 00556 1 0 PERMIT ********

- ********

15 20 MG/l TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH *-*------*------ --* ***---;;; .. ;;:;------** RCORDR FLOW, IN CONDUIT OR THRU SAMPLE 1.073 1.165 ********

0 31/31 03 ** TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD ********

.. SEE RCORDR EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX PERMIT --* SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT 1-* REQUIREMENT

*-----SAMPLE PERMIT REQUIREMENT I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my {

TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the c . 'd t Sequoyah Site Vice President information, the information submitted is . to the best of my knowledge and belief, true. accurate. IT'7l .. res1 en 423 843-7001 12 09 10 and complete. I am aware that there are significant penalties for submitting false information, OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OF iER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} ___ _ Address P.O. BOX 2000 _______ _ Facilitv TVA-SEQUOYAH NUCLEAR PLANT ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ TN0026450 PERMIT NUMBER 110 G DISCHARGE NUMBER DAY 31 MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE I XX I *** Form Approved.

OMB No. 2040-0004 NOTE: Read instructions before completin!l this form PARAMETER X QUANTITI OR LOADING QUALITI OR CONCENTRATION NO. FREQUENCY SAMPLE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE TEMPERATURE, WATER DEG. SAMPLE ********

- CENTIGRADE MEASUREMENT 00010 1 0 PERMIT ********
- ********

EFFLUENT GROSS VALUE REQUIREMENT --TEMPERATURE, WATER DEG. SAMPLE ********

- CENTIGRADE MEASUREMENT 00010 z 0 PERMIT ******** ********
- ********

INSTREAM MONITORING REQUIREMENT s"AMPL_E ___ -----* ---------*-***

-------TEMP. DIFF. BETWEEN SAMP. & ********
- UPSTRM DEG.C MEASUREMENT 00016 1 0 PERMIT ******** ********
- ********

EFFLUENT GROSS VALUE REQUIREMENT

f-------** FLOW, IN CONDUIT OR THRU SAMPLE ********

03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** EFFLUENT GROSS VALUE REQUIREMENT DAILYMX *---------

CHLORINE, TOTAL RESIDUAL SAMPLE ********

- MEASUREMENT 50060 1 0 PERMIT ********
- ********

EFFLUENT GROSS VALUE REQUIREMENT

***--* TEMPERATURE-C, RATE OF SAMPLE ********

04 CHANGE MEASUREMENT f---82234 1 0 PERMIT ******** 2 DEGC ******** EFFLUENT GROSS VALUE REQUIREMENT DAILYMX SAMPLE MEASUREMENT PERMIT REQUIREMENT -** -NAME/TITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin I Certify under penalty of law that this document and all atlachments were prepared under my direction or supervision in accordance wllh a system designed to assure that qualified personne properly gather and evaluate the information submitled. Based on my inquiry of lhe person or ********

********

--!-----*** 0.1 MCH\VG ********

- - - - MAXIMUM REPORT DAILYMX 30.5 DAILYMX 5 DAILYMX ********

0.1 DAILYMX ********

persons who manage the system, or those persons directly responsible for gathering lhe ICe President informalion, the information submitled is , to lhe best of my knowledge and belief, true, accurate, f-\:-=:-:n&.':-::'::::-:=-::-:=::::::=-:c:-:===::-:=---1 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitling false information, J-----=-:=::::-:::-::--::c::-:::-::==--------jincluding the possibility of fine and imprisonment for knowing violations. TIPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used EX OF TIPE UNITS ANALYSIS 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous 04 DEGC CONTIN CALCTD uous ** .. CONTIN RCORDR uous *-f---19 MG/L Five per CALCTD Week ** .. CONTIN CALC TO uous I TELEPHONE DATE 423 843-7001 12 09 10 AREA NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Address P.O. BOX 2000 ---(iNTEROFFICE OPS-5N.SQN)-

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 110 T MAJOR (SUBR 01) F-FINAL Form Approved.

OMB No. 2040-0004 _______ _

=

PERMIT NUMBER DISCHARGE NUMBER I I RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE I XX I *** ATTN: Brad Love YEAR MO DR MO DAY From 12 I 08 oL To 112 08 I NOTE: Read instructions before completinQ this form PARAMETER X QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM IC25 STATRE 7DAY CHR SAMPLE ********
- CERIODAPHNIA f--* **** TRP3B 1 0 0 PERMIT ********

43.2 EFFLUENT GROSS VALUE REQUIREMENT MINIMUM **---***-*

--------*

IC25 STATRE 7DAY CHR SAMPLE ********

- PIMEPHALES TRP6C 1 0 0 PERMIT ********
  - - 43.2 EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ------------SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT

SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the QUALITY OR CONCENTRATION AVERAGE MAXIMUM ********

--********

/ information, the information submitted is

to the best of my knowledge and belief, true, accurate, and complete.

I am aware that there are significant penalties for submitting false information. Sequoyah Site Vice President a 1 President SIGNA OF PRINCIPAL EXECUTIVE 1-------,---------------iincluding the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used OFFICER OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL TELEPHONE DATE 423 843-7001 12 09 10 AREA NUMBER MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) TVA-SEQUOYAH NUCLEAR PLANT NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 118 G MAJOR (SUBR 01) F-FINAL Form Approved. OMS No. 2040-0004 PERMIT NUMBER DISCHARGE NUMBER I WASTEWATER & STORM WATER EFFLUENT ATTN: Brad Love YEAR I MOD y I I MO I DAY From 12 08 01 Tor 12 I 08 I 31 *** NO DISCHARGE I XX I *** NOTE: Read instructions before completin!l this form. PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE ********

MEASUREMENT -19 00300 1 0 PERMIT ********

--2 ********

MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK ----SOLIDS, TOTAL SUSPENDED SAMPLE ********

- - - - ******** ********

-19 MEASUREMENT 00530 1 0 PERMIT ********

--******** ******** 100 MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX WEEK *---------SOLIDS, SETTLEABLE SAMPLE ********

-25 MEASUREMENT 00545 1 0 PERMIT ********

--********

1 ML/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX MONTH ---------FLOW, IN CONDUIT OR THRU SAMPLE ******** ********

03 -TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ********

ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT

_MOAVG __ DAILYMX BATCH --SAMPLE MEASUREMENT PERMIT REQUIREMENT -SAMPLE MEASUREMENT PERMIT REQUIREMENT -SAMPLE MEASUREMENT PERMIT REQUIREMENT I ----NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the 7 .,_. 1 e resident Sequoyah Site Vice President information. the information submitted is

to the best of my knowledge and belief. true, accurate, 423 843-7001 12 09 10 and complete.

I am aware that there are significant penalties for submitting false Information. SIGNAUJP(E OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 S58 12101 0 800-NPDES CORRESPONDENCE October 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37219

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-DISCHARGE MONITORING REPORT (DMR) FOR SEPTEMBER 2012 AND REVISED DMR FOR AUGUST 2012 Enclosed is the September 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The toxicity report and revision to Discharge Number 101T, Biomonitoring for Outfall101 for August 2012, are also attached. There were no exceedances during the monitoring period. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or* by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D. Booker, MOB 1 F-WBN B. E. Brickhouse, BR 4A-C J. T. Carlin, OPS 4A-SQN J. A. Cross, POB 2A-SQN T.R. Markum, BR 4A-C D. B. Nida, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 J.W. Proffitt, OPS 4C-SQN A. A. Ray, WT 11A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1 E-M Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 October 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville,Tennessee 37219

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-DISCHARGE MONITORING REPORT (DMR) FOR SEPTEMBER 2012 AND REVISED DMR FOR AUGUST 2012 Enclosed is the September 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The toxicity report and revision to Discharge Number 101T, Biomonitoring for Outfall101 for August 2012, are also attached. There were no exceedances during the monitoring period. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at {423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. \ Site ic resident Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAl POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMRJ (SUBR01) OMB No. 2040-0004 Addres.l!__f.O. BOX2000 -------------- TN0026450 101 G F-FINAL

--_

PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE

_____ I EFFLUENT -NO DISCHARGE o-ATTN: Brad Love From: 12 : To\12 I NOTE* Read ilistructlons before completing this fonn PARAMETER >< QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ********

41.3 0 30/30 RCORDR -04 CENTIGRADE 00010 1 0 PERMIT .......... ........ ---** ........... Req.Mon

DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT

'. DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE ********

29.1 0 30/30 MODELD ** 04 CENTIGRADE 00010 z 0 PERMIT .. _ .. ........ -... ..... .. ...... 30.5 DEG.C. CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAILYMX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ********

3 o4 0 30/30 CALCTD ** UPSTRM DEG.C 00016 1 s PERMIT ***-* **-*** -........ .._ .. 3 DEG.C. CONTI CALC TO EFFLUENT GROSS REQUIREMENT DAILYMX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** 1761 ******** ********

- - - - "

0 30/30 RCORDR 03 ** PLANT 50050 1 0 PERMIT **-** Req. Mon. MGD ........ ........ . ....... .... CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ********

0.017 0.032 0 24/30 GRAB -19 50060 1 0 PERMIT ********

-*** *-........

0.1 0.1 MGIL FIVE PER CALCTD EFFLUENT GROSS REQUIREMENT MOAVG *DAILYMAX WEEK TEMPERATURE-C, RATE OF SAMPLE ******** 0 ********

0 30/30 CALCTD 62 ** CHANGE 82234 1 0 PERMIT .... -2 DEG *-**** **---**** .... .CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILYMX C/HR NUOUS SAMPLE PERMIT REQUIREMENT

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin I Certify under penally of law that this document and all attachments were prepared under my v n diredion or supervision in accordance with a &Ystem designed*

to assure that qualified personnel ' 1} TELEPHONE DATE properly gather and evaluate the information submitted. Based on my inquiry of the person or ( /."1 f A _ ; . persons who manage the system, or those persons directly responsible for gathering the < * ::..1 Vice President Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submiHing false information, OF PRINCIPAL EXECUTIVE (-----====--==c====-------!including the possibility of fine and Imprisonment tor knowing violations. OFFicER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments !Jere) 423 843-7001 I AREA I NUMBER CODE . 12 10 MO No closed mode operation. Veliger monitoring data is included as ari attachment.' The following injections occurred:

1. Flogoard MS6236 (max. calc. cone. was 0.057mgll-limit 0.2mgll)

2. Biodetergent 73551 (max. calc. cone. was 0.038mg/L-Iimit 2.0mg/L)

3. Spectrus CT1300 (max. calc. cone. was 0.037mgll-limit 0.050mg/L)

EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 10 DAY Mean# of Water

Mean# of Water NOTES;% Sample Date ZM/m3 %Settlers Temp.("C)

Sample Date Asiatic:

Temp.("C)

LOCATION SUB LOCATION Gravid Asiatic: COLLECTED BY Clams/m3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB 01/24/2012 0 0 13 01/24/2012 0 .13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 PB 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234. PB 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May2012 No Samples Collected June 2012 No Samples Collected July2012 No Samples Collected August2012 No Samples Collected September 2012 No Samples Collected PERMmEE NAME/ADDRESS (Include FacilitY Name/Location if Different) Name TVA-SEQUOYAH NUCLEAR PLANT . Address P.O.BOX2000

==

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) F-FINAL Foim Approved.

OMB No. 2040-0004 BIOMONITORING FOR OUTFALL 101 EFFLUENT ATTN: Brad Love *** NO DISCHARGE D *** NOTE: Read instructions before completing this form. PARAMETER L>< QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM IC25 STATRE 7DAY CHR SAMPLE ********

Monitoring

- CERIODAPHNIA Not Req_uired TRP3B 1 0 PERMIT ********
-**** -
- - - - -EFFLUENT GROSS REQUIREMENT MINIMUM IC25 STATRE 7DAY CHR SAMPLE ********

Monitoring

- PIMEPHALES Not Required TRP6C 1 0 PERMIT ********
- -** -'43.2 ********

EFFLUENT GROSS REQUIREMENT MIMINUM SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT 'SAMPLE PERMIT REQUIREMENT £J.. NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of Jaw that this document end all aHachments were prepared under my direction or supervision In accoidance with a system designed to assure that qualified personnel ((X '/ . John T. Carlin properly gather and evaluate the info!lllation submitted. Based on my Inquiry of the. person or persons who manage the system, or those persons dlreclly responsible for gathering the Sequoyah Site Vice President information, the information submilled is , to the beat of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including tha*possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in September 2012. EPA Fonn 3320-1 (REV 3/99) Previous editions may be used l JJJ . -vtce President PRINCIPAL EXECUTIVE OFFIC OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL .. TELEPHONE DATE 423 843-7001 12 10 10 l AREA I NUMBER MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR01) F-FINAL Form Approved. OMB No. 2040-0004 AddreS!..._ ...f.Q...BOX 2000 __ __ ATTN: Brad Love PARAMETER C>< PH SAMPLE 00400 1 0 PERMIT

TN0026450

. QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM ********

"* 7 --------............ "* 6 103 G LOW VOL. WASTE TREATMENT POND EFFLUENT -NO DISCHARGE D -NOTE* Read instructions before completing this form QUALITY OR CONCENTRATION NO. FREQUENCY EX OF AVERAGE MAXIMUM UNITS ANALYSIS

9 0 19/30 12 --------9 su 111REEI SAMF'LE TYPE GRAB GRAB EFFLUENT GROSS REQUIREMENT MINIMUM .. MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED

9 14 0 2/30 GRAB "* 19 00530 1 0 PERMIT ------*-*" "* ......... 30 100 MG/L 1WICEI GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH OIL AND GREASE

- '!'*****

<1 <1 0 2/30 GRAB "* 19

00556 1 0 PERMIT ******" .. --* "* ..........

15 20 MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX. MONTH FLOW, IN CONDUIT OR THRU 0.948 1.134 ******** ********

0 30/30 RCORDR 03 "* TREATMENT PUNT 50050 1 0 PERMIT Req.Mon. Req. Mon MGD ********

- -** ********

... SEE RCORDR EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX PERMIT MEASUREMENT PERMIT REQUIREMENT MEASUREMENT PERMrr REQUIREMENT ' SAMPLE MEASUREMENT PERMIT REQUIREMENT

/ * \r)r, n NAME/TITLE PRINCIPAL EXECUTNE OFFICER I Certify under penalty of Jaw that this document and all attachments were prepared under my 1/ I TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted.

Based on my inquiry of the person or I;:;_ persons who manage the system, or those persons directly responsible for gathering the 1\ Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 12 10 10 and complete. I am aware that there are significant penalties for submitting false information, PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. IC OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPlANATION OF ANY (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Address _ _E.Q...BOX2000 ____ _ ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 110 G PERMIT NUMBER DISCHARGE NUMBER MO YEA MO {)AY . I . I Fro.m 12 I 09 I 01 I To !12 I 09 I 30 MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE rxx I *** Fonn Approved.

OMB No. 2040-0004 NOTE* Read instructions before completinR this fonn PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM TEMPERATURE, WATER DEG. SAMPLE ********

- - - .'****
- CENTIGRADE 00010 1 0 PERMIT ********
- ********

. ....... REPORT EFFLUENT GROSS VALUE REQUIREMENT. DAILYMX TEMPERATURE, WATER DEG. . SAMPLE ********

'********

- CENTIGRADE 00010 z 0 PERMIT --******
- -** ****-***

30.5 INSTREAM MONITORING REQUIREMENT DAILYMX TEMP. DIFF. BETWEEN SAMP. & SAMPLE ********

- UPSTRM DEG.C 00016 1 0 PERMIT .... _ ........
- ********

........ 5 EFFLUENT GROSS VALUE REQUIREMENT DAILYMX FLOW, IN CONDUIT OR THRU SAMPLE ********

- -******

03 PUNT 50050 1 0 PERMIT ........ Req. Mon

MGD ********

........

EFFLUENT GROSS VALUE REQUIREMENT DAILYMX CHLORINE, TOTAL RESIDUAL SAMPLE ********

'********

50060 1 0 . PERMIT ........

- ********

0.1 0.1 EFFLUENT GROSS VALUE REQUIREMENT

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EFFLUENT GROSS VALUE REQUIREMENT DAILYMX SAMPLE PERMIT REQUIREMENT ......---.... r\1_1 fJ._ NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law tl)at this documarif and all attachments wera praperad under my let *-;deot direction or supervision In ai:cordance with a system dasigned to assure that qualified John T. Carlin properly gathar and avaluata tha information submitted. Based on my inquiry of tha person or persons who managa tha system, or those persons directly responsible for gathering the . .. Sequoyah Site Vice President information

. the information is , to tha bast of my knowledge and belief, true, SC!'Urata.

and complate. I am awera that thara ara significant penalties for submitting falsa information, Including tha possibility of !ina and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here} No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT EX OF TYPE UNITS ANALYSIS 04 DEGC CONTIN CALCTD uous 04 DEGC CO NT IN CALCTD uous 04 DEGC CONTIN CALC TO uous ** ... CONTIN RCORDR uous 19 MGIL Five per CALCTD Week ** ... CONTIN CALCTD uous ' TELEPHONE DATE 423 843-7001 12 10 10 I AREA I NUMBER MO DAY CODE Page 1 of 1 PERMmEE NAME/ADDRESS (Include Facility NameJLoca(;on if Different} Name TVA-SEQUOYAH NUCLEAR PLANT Address P.O. BOX2000 ============ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDE.S} DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR01) F-FINAL Fonn Approved. OMB No. 2040-0004 RECYCLED COOLING WATER EFFLUENT A TIN: Brad Love *** NO DISCHARGE I XX I *** NOTE: Read instructions before completing this fonn PARAMETER QUANnTY OR LOADING QUALITY OR CONCENTRATION X AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM IC25 STATRE 7DAY CHR SAMPLE '********

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EFFLUENT GROSS VALUE REQUIREMENT MINIMUM SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT* REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OF'FICER I Certify under penally of law that this document end all attachments -re prepared under my direction or supervision In accordance with a systam designed to assure that qualified personnel John T. Carlin properly gather and evaluata the information submitted. Based on my inquiry of the person or persons who manage the system, or thosa persons directly responsibla for gathering tha Sequoyah Site Vice President information, the information submitted is , to the best of my knowiedga and belief, ln!e. accurate, and complete. I ain aware that there are significant penalties for submitting false information, including the possibility of fine and Imprisonment for knowing violations. lYPED OR PRINTED COMMENTS AND OF ANY (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) PreVious editions may be used / }..,-V\ /} (Arw "[{J& President SIGNA;r'URE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL TELEPHONE DATE 423 843-7001 12 10 10 I AREA I NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility NameA.ocatlon if Different) ___ _ Addres!._ _e.Q,_BOX 2000 __________ . __ ..:...._ __ .J!.NTEROFFICE OPS-5N-SQID-'- _____ _ ____ _ ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT . (DMR) TN0026450 118 G PERMIT NUMBER DISCHARGE NUMBER YEAR MO MO DAY I . I From 12 I 09 o1 To 112 I 09 I 30 MAJOR (SUBR01) F-FINAL WASTEWATER & STORM WATER EFFLUENT

- - NO DISCHARGE I XX I *** Form Approved.

OMB No. 2040-0004 NOTE: Read Instructions tiefore completing this form. PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE ******** MEASUREMENT

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. -,....... NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under JlEII!ally of law that this doaJment and all aHachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system deaigned to easure that qualified personnal John T. Carlin properly gather and evaluate the information submitted. Baaed on my inquiry of the person or persons who manage the system, or thOSe persons directly reaponslbla for gathering tha Sequoyah Site Vice President information, the information submitted is , to the beat of my knowiedge and belief, true, accurate, 423 843-7001 12 10 10 and complate. I am aware that there are significant penaltiea for submitting false information, SIGNA 'tORE OF PRINCIPAL EXECUTNE I lnduding the possibility of fine and imprisonmant for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REv 3199) Previous editions may be used Page 1 of 1 NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR01) F-FINAL Form Approved. OMB No. 2040-0004 BIOMONITORING FOR OUTFALL 101 EFFLUENT ATTN: Brad Love *** NO DISCHARGE D *** NOTE* Read instnictions before complelina this form PARAMETER X QUANnTY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM IC25 STATRE 7DAY CHR SAMPLE ********

>100.0 ** CERIODAPHNIA MEASUREMENT TRP3B 1 0 PERMIT ********

-43.2 EFFLUENT GROSS REQUIREMENT MINIMUM JC25 STATRE 7DAY CHR SAMPLE ********

>100.0 ** PIMEPHALES MEASUREMENT TRP6C 1 0 PERMIT ********

...... 43.2 EFFLUENT GROSS REQUIREMENT MIMINUM SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT *REQUIREMENT NAME/TITLE PRINCIPAL EXECUnVE: OFFICER I Certify under penalty of lew that this document and all eHachments were prepared un.der my direclion or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and 'evaluate the information submitted. Based on my inquiry of the.person or QUALITY OR CONCENTRAnON AVERAGE MAXIMUM ********

_ ...... .. ********

.. ********

parsons who manage the system, or those parsilns directly responsible for gathering the President Sequoyah Site Vice President information, the information submitted Is. to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties tor submitting false information, SIG E OF PRINCIPAL. EXECUTIVE 1------------------Jincluding the possibility of line and imprisonment for knowing violations. . OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity sampling began on August 12 and ended on August 17. This is the revised August 2012 DMR data set for 101T. The toxicity report is attached. EPA Fonn 3320-1 (REV 3199) PreVious editions may be used NO. FREQUENCY SAMPLE EX *oF TYPE UNITS ANALYSIS 23 0 1/180 COMPOS PERCENT SEMI COMPOS ANNUAL 23 0 1/180 COMPOS PERCENT .SEMI COMPOS ANNUAL TELEPHONE DATE 423 843-7001 12 10 10 AREA NUMBER YEAR MO DAY CODE Page 1 of 1 TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE SUMMARY Report Date: September 17, 2012

1. Facility / Discharger: Sequoyah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1,579

6. Receiving Stream: Tennessee River (TRM 483.6)

7. 1Q10: 3,491

8. Outfall Tested: 101

9. Dates Sampled: August 12 - 17, 2012

10. Average Flow on Days Sampled (MGD): 1741.838, 1748.488, 1747.571

11. Pertinent Site Conditions: Production / operation data will be provided upon request.

12. Test Dates: August 14 - 21, 2012

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (

Pimephales promelas ) Daphnids ( Ceriodaphnia dubia )

15. Concentrations Tested (%): Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100 Pimephales promelas

UV treated Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100

16. Permit Limit Endpoint (%): Outfall 101: IC 25 = 43.2%

17. Test Results: Outfall 101

Pimephales promelas:

IC25 > 100% Ceriodaphnia dubia: IC25 > 100% UV treated Outfall 101

Pimephales promelas:

IC25 > 100%

18. Facility Contact: Brad Love Phone #: (423) 843-6714

19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.

20. Lab Contact: Jim Sumner Phone #: (828) 350-9364

21. TVA Contact: Donald W. Snodgrass Phone #: (256) 386-2787

22. Notes: Outfall 101 samples collected Augus t 12 - 17, 2012, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the c ontrols during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been th e suspected cause of interference (anomalous dose response and high variability among replicates) in prev ious toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

METHODS SUMMARY Samples:

1. Sampling Point: Outfall 101, Intake

2. Sample Type: Composite

3. Sample Information:

Sample ID Date (MM-DD-YY) Time (ET) Collected Date (MM-DD-YY) Time (ET) Received Arrival Temp. (°C) Initial TRC* (mg/L) Date (MM-DD-YY) Time (ET) Last Used By 101 08-12-12 0645 to 08-13-12 0545 08-13-12 1540 0.8, 0.7 f <0.10 08-14-12 1213

08-15-12 1115 Intake 08-12-12 0705 to 08-13-12 0605 08-13-12 1540 0.9 <0.10 08-14-12 1213

08-15-12 1115 101 08-14-12 0700 to 08-15-12 0600 08-15-12 1553 1.9, 1.9 f <0.10 08-16-12 1114

08-17-12 1115 Intake 08-14-12 0720 to 08-15-12 0620 08-15-12 1553 2.0 <0.10 08-16-12 1114

08-17-12 1115 101 08-16-12 0700 to 08-17-12 0600 08-17-12 1338 2.2, 2.2 f <0.10 08-18-12 1127

08-19-12 1115

08-20-12 1114 Intake 08-16-12 0715 to 08-17-12 0615 08-17-12 1338 2.4 <0.10 08-18-12 1127

08-19-12 1115

08-20-12 1114 *TRC = Total Residual Chlorine fSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0 oC) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Pimephales promelas Ceriodaphnia dubia Test Organisms:

1. Source: Aquatox, Inc.

In-house Cultures

2. Age: 20 - 20.22 hours old

< 24-hours old Test Method Summary:

1. Test Conditions: Static, Renewal Static, Renewal

2. Test Duration: 7 days Until at least 60% of control females have 3 broods

3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic

4. Number of Replicates: 4 10

5. Organisms per Replicate: 10 1

6. Test Initiation: (Date/Time)

Outfall 101 08-14-12 1200 ET 08-14-12 1148 ET UV Treated Outfall 101 08-14-12 1213 ET

7. Test Termination: (Date/Time)

Outfall 101 08-21-12 1106 ET 08-21-12 1053 ET UV Treated Outfall 101 08-21-12 1122 ET

8. Test Temperature: Outfall 101: Mean = 24.7 oC Mean = 24.9 oC (24.3 - 25.1 oC) (24.6 - 25.3 oC) Test Temperature: UV-Treated Outfall 101: Mean = 24.8 oC (24.2 - 25.1 oC) 9. Physical / Chemical Measurements: Alkalinity, hardness, tota l residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistica l software (Tidepool Scientific Software, McKinneyville, CA).

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test

. (Genus species) (Type / Duration)

Conducted August 14 - 21, 2012 using effluent from Outfall 101 . Test Solutions (% Effluent) Percent Surviving (time interval used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 95 Test Solutions (% Effluent) Mean Dry Weight (mg) (replicate number) 1 2 3 4 Mean Control 0.583 0.615 0.592 0.544 0.584 10.8% 0.535 0.669 0.686 0.649 0.635 21.6% 0.608 0.559 0.626 0.686 0.620 43.2% 0.582 0.563 0.487 0.614 0.562 86.4% 0.614 0.555 0.563 0.690 0.606 100.0% 0.532 0.673 0.506 0.571 0.571 Intake 0.537 0.569 0.551 0.687 0.586 IC25 Value: > 100% Permit Limit: 43.2%

95% Confidence Limits: Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 2.3 TUc

   *TUa = 100/LC 50: TUc = 100/ IC25 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test

. (Genus species) (Type / Duration) Conducted August 14 - 21, 2012 using effluent from Outfall 101. Test Solutions (% Effluent) Percent Surviving (time interval used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions (% Effluent) Reproduction (#young/female/7 days) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 MeanControl 30 33 29 33 31 28 34 32 30 32 31.2 10.8% 36 34 33 33 35 35 34 34 31 32 33.7 21.6% 36 31 33 31 30 37 35 33 32 30 32.8 43.2% 37 34 34 31 31 31 37 34 34 35 33.8 86.4% 34 34 36 34 35 33 34 35 36 37 34.8 100.0% 38 37 37 34 32 38 35 39 37 38 36.5 IC25 Value: > 100% Permit Limit: 43.2% 95% Confidence Limits: Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 2.3 TUc

      *TUa = 100/LC 50: TUc = 100/ IC 25 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test

. (Genus species) (Type / Duration)

Conducted August 14 - 21, 2012 using water from Intake Test Solutions (% Effluent) Percent Surviving (time interval used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100

Test Solutions (% Effluent) Reproduction (#young/female/7 days) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 MeanControl 30 34 34 31 32 29 32 31 29 31 31.3 Intake 33 29 33 33 33 36 33 32 36 34 33.2 IC25 Value: > 100% Permit Limit: N/A

95% Confidence Limits: Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/A

      *TUa = 100/LC 50: TUc = 100/ IC 25 TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test

. (Genus species) (Type / Duration)

Conducted August 14 - 21, 2012 using effluent from UV Treated Outfall 101 . Test Solutions (% Effluent) Percent Surviving (time interval used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 98 98 98 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions (% Effluent) Mean Dry Weight (mg) (replicate number) 1 2 3 4 Mean Control 0.510 0.488 0.587 0.553 0.535 10.8% 0.673 0.559 0.631 0.594 0.614 21.6% 0.599 0.524 0.547 0.582 0.563 43.2% 0.592 0.516 0.548 0.588 0.561 86.4% 0.509 0.527 0.551 0.448 0.509 100.0% 0.550 0.565 0.526 0.494 0.534 Intake 0.621 0.573 0.647 0.482 0.581 IC25 Value: > 100%

95% Confidence Limits: Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc*

      *TUa = 100/LC 50: TUc = 100/ IC 25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) Species Date Time DurationToxicant Results (IC

25) Pimephales promelas August 14 - 21, 2012 1225 7 days KCl 0.81 g/L Ceriodaphnia dubia August 07 - 14, 2012 1045 7 days NaCl 1.08 g/L 24.7-24.924.6-25.17.6-7.97.8-8.07.43-7.677.36-7.59302-32362-6288-9224.7-25.024.3-25.07.7-8.07.6-8.07.44-7.627.33-7.55285-298 24.7-25.024.4-24.97.7-8.07.6-8.07.44-7.627.33-7.55272-283 24.7-25.024.4-24.87.7-8.07.6-8.07.45-7.617.34-7.52246-259 24.7-25.024.3-24.87.6-8.07.6-7.97.45-7.607.31-7.52192-20524.8-25.024.4-24.87.7-8.17.7-8.17.42-7.607.32-7.57177-18670-7271-74< 0.10-< 0.10 24.7-25.024.4-24.77.7-8.27.9-8.07.43-7.617.33-7.54176-18668-7072-74< 0.10-< 0.10 24.7-24.925.0-25.27.6-7.97.8-8.27.43-7.677.28-7.59302-32362-6288-9224.6-25.024.8-25.27.7-8.07.8-8.27.44-7.627.29-7.59285-298 24.7-25.024.8-25.17.7-8.07.8-8.27.44-7.627.30-7.60272-283 24.7-25.024.8-25.37.7-8.07.9-8.27.45-7.617.30-7.61246-259 24.7-25.124.8-25.27.6-8.07.9-8.27.45-7.607.28-7.61192-20524.8-25.124.8-25.27.7-8.17.9-8.37.42-7.607.29-7.63177-18670-7271-74< 0.10-< 0.1024.8-25.124.7-25.17.7-8.28.0-8.27.43-7.617.30-7.64176-18668-7072-74< 0.10-< 0.10Overall temperature (

oC)Pimephales promelasCeriodaphnia dubia< 0.10< 0.1018225.0727.518.18.018273100.0%25.024.97.97.52697.528.08.07.51717.517.51198252 2787.925.07.98.07.537.527.5224.924.97.98.07.5462896973< 0.107.98.07.527.512917.513117.55Ceriodaphnia dubiaControl24.825.1Intake21.6%86.4%24.925.024.818224.924.68.08.07.517.45Intake7.78.0100.0%24.910.8%24.825.043.2%24.91827.87.517.421987.467.97.51< 0.10 71727.447.8Total ResidualChlorine (mg/L)Conductance(mg/L CaCO 3)(mg/L CaCO 3)Pimephales promelasControl24.824.710.8%86.4%24.8627.557.97.97.77.537.825243.2%24.824.67.507.467.921.6%24.824.67.4424.67.9(µmhos/cm)311897.547.5229127824.824.67.87.924.67.9InitialFinalInitialFinalInitialFinalPHYSICAL/CHEMICAL SUMMARYperformed August 14-21, 2012.TestSample IDTemperature ( oC)Dissolved Oxygen (mg/L)pH (S.U.)AlkalinityHardnessWater Chemistry Mean Values and Ranges for Pimephales promelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfall 10124.924.625.3AverageMinimumMaximum24.724.325.1 24.8-24.924.5-24.97.8-8.17.6-7.97.50-7.657.34-7.61298-31661-6284-8424.8-25.024.5-25.07.9-8.07.6-7.87.50-7.667.34-7.57282-298 24.8-25.124.6-24.87.9-8.07.7-7.97.50-7.667.34-7.58271-284 24.8-25.124.6-24.87.9-8.07.7-7.97.49-7.667.33-7.58246-258 24.9-25.124.3-24.87.9-8.17.5-7.97.48-7.637.33-7.57197-20725.0-25.124.2-24.77.8-8.37.5-7.97.47-7.627.31-7.55178-18870-7072-72< 0.10-< 0.1024.8-25.124.5-24.97.9-8.27.7-8.07.46-7.617.32-7.58178-19170-7071-72< 0.10-< 0.10*Note: Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.Overall temperature ( oC)Pimephales promelasAverageMinimumMaximum24.824.225.1PHYSICAL/CHEMICAL SUMMARYperformed August 14-21, 2 012.TestSample IDTemperature ( oC)Dissolved Oxygen (mg/L)pH (S.U.)AlkalinityHardnessWater Chemistry Mean Values and Ranges for Pimephales promelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 101InitialFinalInitialFinalInitialFinal 24.924.77.87.924.78.07.87.97.97.59 7.6029127943.2%24.924.77.497.487.821.6%24.924.77.597.97.587.8Pimephales promelasControl24.824.710.8%86.4%25.0*Total ResidualChlorine (mg/L)Conductance(mg/L CaCO 3)(mg/L CaCO 3)61(µmhos/cm)304847.55< 0.102547.477.487.97.547.461847.87.567.462037.457.870727072< 0.10Intake100.0%25.118425.024.68.124.68.0 SUMMARY / CONCLUSIONS Outfall 101 samples collected August 12 - 17, 2012, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the c ontrols during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been th e suspected cause of interference (anomalous dose response and high variability among replicates) in prev ious toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

Appendix A ADDITIONAL TOXICITY TEST INFORMATION SUMMARY OF METHODS

1. Pimephales promelas Tests were conducted according to EP A-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None 2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PR ETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None 2. Ceriodaphnia dubia None PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identific ation, service, and calibrati on information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B.

4. Dissolved oxygen was measured by SM 4500 O G.

5. The pH was measured by SM 4500 H+ B.

6. Conductance was measured by SM 2510 B.

7. Alkalinity was measured by SM 2320 B.

8. Total hardness was measured by SM 2340 C.

9. Total residual chlorine was meas ured by ORION Electrode Method 97-70.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic test s with results expressed as IC 25 values in g/L KCl or NaCl.

2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas

. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderate ly hard synthetic water.

4. Statistics: ToxCalc software Versi on 5.0 was used for statistical analyses.

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms

, EPA-821-R-02-013 (October 2002).

3. Standard Methods for the Examination of Water and Wastewater, 21 st Edition, 2005.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (mos t current version).

Sequoyah Nuclear Plant Biomonitoring August 14 - 21, 2012

Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to C ontrol Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrommg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130Mmg/L Quat 03/12/1998 0.016 - - - - - - 03/13/1998 0.015 - - - - - - 03/14/1998 0.013 - - - - - - 03/15/1998 0.030 - - - - - - 03/16/1998 0.013 - - - - - - 03/17/1998 0.020 - - - - - - 03/18/1998 0.018 - - - - - - 09/08/1998 0.015 - 0.014 0.005 - - 0.021 09/09/1998 0.003 - 0.031 0.011 - - - 09/10/1998 0.014 - 0.060 0.021 - - - 09/11/1998 0.013 - 0.055 0.019 - - - 09/12/1998 < 0.001 - 0.044 0.015 - - - 09/13/1998 < 0.001 - 0.044 0.015 - - - 09/14/1998 0.008 - 0.044 0.015 - - - 02/22/1999 < 0.001 - - - - - - 02/23/1999 0.005 - - - - - - 02/24/1999 0.009 - - - - - - 02/25/1999 0.012 - - - - - - 02/26/1999 0.008 - - - - - - 02/27/1999 < 0.001 - - - - - - 02/28/1999 < 0.001 - - - - - - 08/18/1999 - 0.015 0.069 0.024 0.006 - - 08/19/1999 - 0.012 0.068 0.024 - - - 08/20/1999 - 0.023 0.070 0.024 - 0.120 - 08/21/1999 - 0.022 0.068 0.024 - - - 08/22/1999 - 0.022 0.068 0.024 - - - 08/23/1999 - 0.025 0.068 0.024 0.006 - - 08/24/1999 - 0.016 0.067 0.023 0.020 - - Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosi on Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrommg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130Mmg/L Quat 01/31/2000 - < 0.002 0.026 0.009 - - - 02/01/2000 - 0.011 0.026 0.028 - - - 02/02/2000 - 0.028 0.026 0.009 0.006 - - 02/03/2000 - 0.008 0.027 0.009 - - - 02/04/2000 - 0.006 0.027 0.009 0.005 0.109 - 02/05/2000 - < 0.002 0.027 0.009 - - - 02/06/2000 - < 0.002 0.027 0.009 - - - 07/26/2000 - < 0.0057 0.055 0.019 - - - 07/27/2000 - 0.019 0.055 0.019 - - - 07/28/2000 - 0.0088 0.053 0.018 0.004 0.108 - 07/29/2000 - < 0.0088 0.055 0.019 - - - 07/30/2000 - < 0.0076 0.055 0.019 - - - 07/31/2000 - < 0.0152 0.055 0.019 0.006 - - 08/01/2000 - < 0.0141 0.055 0.019 0.005 - - 12/11/2000 - 0.0143 0.025 0.020 0.005 - - 12/12/2000 - 0.0092 0.025 0.020 0.005 - - 12/13/2000 - < 0.0120 0.025 0.020 - - - 12/14/2000 - < 0.0087 0.025 0.020 - - - 12/15/2000 - 0.0120 0.025 0.020 0.005 - - 12/16/2000 - < 0.0036 0.025 0.020 - - - 12/17/2000 - < 0.0036 0.025 0.020 - - - 08/26/2001 - 0.017 0.06 0.021 0.006 - - 08/27/2001 - <0.0096 0.06 0.021 0.005 - 0.021 08/28/2001 - <0.0085 0.06 0.021 - - - 08/29/2001 - <0.0094 0.059 0.020 0.005 - 0.021 08/30/2001 - <0.0123 0.06 0.021 0.005 - - 08/31/2001 - <0.005 0.059 0.020 - - - 11/25/2001 - <0.0044 - - - - - 11/26/2001 - <0.0119 0.024 0.02 0.005 - - 11/27/2001 - 0.0137 0.023 0.019 0.007 - - 11/28/2001 - <0.0089 0.022 0.019 0.006 - - 11/29/2001 - 0.0132 0.024 0.02 0.007 - - 11/30/2001 - < 0.0043 0.024 0.02 - - - 12/09/2001 - <0.0042 - - - - - 12/10/2001 - <0.0042 - - - - - 12/11/2001 - <0.0104 - - - - - 12/12/2001 - 0.0128 0.024 0.02 0.008 - - 12/13/2001 - <0.0088 0.024 0.02 - - - 12/14/2001 - 0.0134 0.024 0.02 0.007 - - Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosi on Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrommg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130Mmg/L Quat 01/02/2002 - < 0.0079 0.023 0.02 0.006 - - 01/03/2002 01/04/2002 01/05/2002 01/06/2002 01/07/2002 - - - - - < 0.0042 0.0124 < 0.0042 < 0.0042 < 0.00890.023 0.024 - - 0.0240.014 0.014 - - 0.014- 0.009 - - 0.006 - - - - - - - - - - 02/24/2002 02/25/2002 02/26/2002 02/27/2002 02/28/2002 03/01/2002 - - - - - - < 0.004 < 0.004 0.0143 < 0.0041 < 0.0041 < 0.0041 - 0.023 0.023 0.023 0.024 0.024 - 0.023 0.023 0.023 0.008 0.008 - - 0.007 - - - - - - - - - - - - - - - 05/05/2002 05/06/2002 05/07/2002 05/08/2002 05/09/2002 05/10/2002 - - - - - - - - - - - - - 0.058 0.058 0.056 0.057 0.056 - 0.02 0.02 0.019 0.02 0.019 - 0.014 0.015 - 0.014 - - - - - - - - - - - - - 08/04/2002 08/05/2002 08/06/2002 08/07/2002 08/08/2002 08/09/2002 - - - - - - <0.0058 <0.0058 0.0092 <0.0107 <0.0061 0.0152 - 0.053 0.053 0.055 0.055 0.054 - 0.018 0.018 0.019 0.019 0.018 - - - 0.007 - 0.008 - - - - - - - 0.025 - - - - 10/06/2002 10/07/2002 10/08/2002 10/09/2002 10/10/2002 10/11/2002 - - - - - - <0.00497 0.0153 <0.0092 0.0124 0.0134 <0.0042 - 0.054 0.054 0.053 0.054 0.054 - 0.018 0.018 0.018 0.018 0.018 - 0.009 0.007 0.009 0.009 - - - - - - - - - - - - - 01/12/2003 01/13/2003 01/14/2003 01/15/2003 01/16/2003 01/17/2003 - - - - - - <0.0035 <0.006 <0.0118 <0.0063 <0.0034 <0.0034 - 0.025 0.026 0.026 0.026 0.026 - 0.019 0.020 0.020 0.020 0.009 - 0.009 0.009 - - - - - - - - - - - - - 04/06/2003 04/07/2003 04/08/2003 04/09/2003 04/10/2003 04/11/2003 - - - - - - <0.0073 <0.0189 <0.0117 <0.0139 <0.0113 <0.0073 - - - - - - - 0.021 0.021 0.021 0.021 0.022 - - - 0.016 0.018 - - - - - - - - - - - - - Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Cont rol Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrommg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130Mmg/L Quat 06/15/2003 - < 0.0045 - - - - - 06/16/2003 06/17/2003 06/18/2003 06/19/2003 06/20/2003 - - - - - < 0.0037 < 0.0048 < 0.0048 < 0.0085 < 0.00480.057 0.041 0.041 0.058 0.0580.020 0.014 0.014 0.020 0.020- - - - - - - - - - 0.022 0.024 0.024 0.025 0.025 08/03/2003 08/04/2003 08/05/2003 08/06/2003 08/07/2003 08/08/2003 - - - - - - <0.0050 <0.0050 <0.0051 <0.0084 0.0129 0.0153 - 0.058 0.057 0.057 0.057 0.057 - 0.020 0.020 0.020 0.020 0.020 - - - - - 0.009 - - - - - - - - 0.025 0.025 0.024 - 10/05/2003 10/06/2003 10/07/2003 10/08/2003 10/09/2003 10/10/2003 - - - - - - <0.0043 <0.0043 <0.0090 <0.0106 0.0181 0.0183 0.057 0.057 0.057 0.057 0.026 0.026 0.020 0.020 0.020 0.020 0.022 0.024 - - - - - 0.009 - - - - - - - 0.025 0.025 0.025 0.025 - 02/01/2004 02/02/2004 02/03/2004 02/04/2004 02/05/2004 02/06/2004 - - - - - - 0.0093 <0.0034 <0.0034 0.0124 <0.0034 0.0105 0.027 0.026 0.026 0.026 0.026 0.026 0.009 0.009 0.009 0.009 0.009 0.009 - - - 0.009 - 0.010 - - - - - - - - - - - - 05/04/2004 05/05/2004 05/06/2004 05/07/2004 05/08/2004 05/09/2004 - - - - - - <0.0123 <0.0144 <0.0146 0.0227 0.016 <0.0104 0.026 0.026 0.037 0.058 0.060 0.058 0.019 0.014 0.013 0.020 0.021 0.020 - 0.009 - 0.009 - - - - - - - - 0.025 0.025 0.025 0.025 - - 07/04/2004 07/05/2004 07/06/2004 07/07/2004 07/08/2004 07/09/2004 - - - - - - 0.0217 <0.0085 <0.0077 0.0252 0.0223 0.0182 0.057 0.057 0.058 0.056 0.057 0.057 0.019 0.020 0.020 0.019 0.019 0.020 - 0.009 - - 0.009 0.009 - - - - - - - - 0.031 0.031 - - Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosi on Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Hypochlorit e mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130M mg/L Quat Nalco 73551 mg/L EO/PO H-150M mg/L Quat 11/07/2004 11/08/2004 11/09/2004 11/10/2004 11/11/2004 11/12/2004 - - - - - - <0.0187 <0.0192 <0.0233 <0.0149 <0.0149 <0.0253 0.000 0.047 0.048 0.047 0.049 0.048 0.014 0.030 0.016 0.016 0.017 0.017 - - - - - - - - - - - - - - - - 0.041 0.041 0.043 0.042 - - - - - - - - - - 02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/11/2005 - - - - - - <0.0042 <0.0116 <0.0080 0.0199 <0.0042 0.0155 0.028 0.028 0.028 0.028 0.028 0.028 0.010 0.010 0.010 0.010 0.010 0.010 - - - - - - - - - - - - - - - - - - - 0.007 - - - 0.007 - - - - - - 06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10/2005 - - - - - - 0.0063 0.0043 0.0103 0.0295 0.0129 0.0184 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.037 0.037 0.037 - - 07/17/2005 07/18/2005 07/19/2005 07/20/2005 07/21/2005 07/22/2005 - - - - - - 0.0109 0.0150 0.0163 0.0209 0.0242 0.0238 0.026 0.026 0.026 0.026 0.026 0.054 0.009 0.009 0.009 0.009 0.009 0.018 - - - - - - - - - - - - - - - - - - - - - 0.014 - 0.014 - 0.036 0.036 0.036 - - 10/30/2005 10/31/2005 11/01/2005 11/02/2005 11/03/2005 11/04/2005 - - - - - - 0.0068 0.0112 0.0104 0.0104 0.0117 0.0165 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.035 0.036 0.036 0.035 11/14/2005 11/15/2005 11/16/2005 11/17/2005 11/18/2005 11/19/2005 - - - - - - 0.0274 0.0256 0.0234 0.0231 0.0200 0.0116 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Cont rol Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrommg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130M mg/L Quat Nalco 73551 mg/L EO/PO H-150Mmg/L Quat MSW 101 mg/L Phosphate 11/12/2006 11/13/2006 11/14/2006 11/15/2006 11/16/2006 11/17/2006 - - - - - - 0.0055 0.0068 0.0143 0.0068 0.0267 0.0222 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.037 0.037 0.037 0.037 - - - - - - - 11/26/2006 11/27/2006 11/28/2006 11/29/2006 11/30/2006 12/01/2006 - - - - - - 0.0188 0.0138 0.0120 0.0288 0.0376 0.0187 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 05/28/07 05/29/07 05/30/07 05/31/07 06/01/07 06/02/07 - - - - - - - - 0.0084 0.0103 0.0164 0.0305 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.017 - 0.017 - - 0.036 0.036 0.036 0.036 - 0.015 0.015 0.015 0.015 0.015 0.015 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 - - - - - - 0.0241 0.0128 0.0238 0.0158 0.0162 0.0175 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 04/13/08 04/14/08 04/15/08 04/16/08 04/17/08 04/18/08 - - - - - - 0.0039 0.0124 0.0229 0.0143 0.0120 0.0149 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 10/26/08 10/27/08 10/28/08 10/29/08 10/30/08 10/31/08 - - - - - - 0.0260 0.0151 0.0172 0.0154 - 0.0086 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.017 - 0.018 - - - - 0.041 0.041 0.041 0.041 - - - 0.030 0.030 0.030 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically In duced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L CopolymerCL-363mg/L DMADCuprostat-PF mg/L Azole H-130Mmg/L Quat Nalco 73551 mg/L EO/PO Spectrus CT1300 mg/L Quat H-150M mg/L Quat MSW 101 mg/L Phosphate02/08/09 02/09/09 02/10/09 02/11/09 02/12/09 02/13/09 - - - - - - 0.0197 0.0237 0.0104 0.0155 0.0106 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.017 0.017 0.021 0.017 0.017 - - - - - - - - - - - - - - - - - - - 05/10/09 05/11/09 05/12/09 05/13/09 05/14/09 05/15/09 - - - - - - 0.0129 0.0415 0.0053 0.0049 <0.0141 <0.0160 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.0446 0.0396 0.0396 0.0397 - - - - - - - 11/15/09 11/16/09 11/17/09 11/18/09 11/19/09 11/20/09 - - - - - - 0.025 0.0152 0.0255 0.0306 0.0204 0.0093 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 05/09/10 05/10/10 05/11/10 05/12/10 05/13/10 05/14/10 - - - - - - 0.0192 0.0055 0.0100 0.0171 0.0041 0.0099 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.039 0.039 0.039 0.039 - - - - - - - - - - - -

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically In duced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Hypo-chlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phos-phate PCL-401 mg/L Copoly-mer CL-363mg/L DMADCuprostat-PF mg/L Azole H-130Mmg/L Quat Nalco 73551 mg/L EO/PO Spectrus CT1300 mg/L Quat H-150Mmg/L Quat MSW 101 mg/L Phos-phate Floguard MS6236mg/L Phosphate10/31/10 11/01/10 11/02/10 11/03/10 11/04/10 11/05/10 - - - - - - - 0.0122 0.0112 0.0163 0.0107 0.0132 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 05/01/2011 05/02/2011 05/03/2011 05/04/2011 05/05/2011 05/06/2011 - - - - - - - - - 0.0155 0.0179 0.0089 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.04 0.04 0.04 0.04 - - - - - - - - - - - - - - - - - - - 11/06/2011 11/07/2011 11/08/2011 11/09/2011 11/10/2011 11/11/2011 - - - - - - 0.0168 0.0225 0.0141 0.0239 0.0242 0.0231 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 05/06/2012 05/07/2012 05/08/2012 05/09/2012 05/10/2012 05/11/2012 - - - - - - - - - 0.0145 0.0298 0.0174 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.041 0.041 0.041 - - - - - - - - - - - - - - - - - - - 08/12/2012 08/13/2012 08/14/2012 08/15/2012 08/16/2012 08/17/2012 - - - - - - - 0.0256 0.0209 0.0279 0.0076 0.0446 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.028 - 0.028 - - - 0.037 0.037 - - - - - - - - - - - - - - - 0.029 0.029 0.029 0.029 0.029 0.032

Sequoyah Nuclear Plant Biomonitoring August 14 - 21, 2012

Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Field Identification I BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of_l_ Environmental Testing Solution, Inc. 3 51 Depot Street. Phone: .Fax: Asheville, NC 28801 828-350-9364 828-350-9368 Container Flow Delivered By (Circle One): FedEx UPS Bus Client Other (specify): ______ _ General Comments: Toofo.. q .,_ o "+-Ff It 4 t o 17c.;a,/ s ryl-c.s _, r o I.,Jo, Number& Rain Event? Sample Description Grab/Comp. Collection Date/Time (MGD) (Mark as Appropriate) Start End Yes If Yes, No Trace Inches 8"1]--12 SQN-101-TOX Comp VCt.tr E' 2(2.5gal) X ,_,,_ ,,_ r-IJ-1'). SQN-INT-TOX Comp o 7PJ' e' 1(2.5 gal) Relinquished By (Signature): Date/Time 0 08

rL 1$'/0 E.\ Instructions:

Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. Whole Efflue( )ample Receipt Log Page __12_( *Sample temperature performed using Sample Receiving Thermometer: SN 6338 Date Time Received Received

Sample Project Sample Sample name and description State Comments Received Received by from Temp. (0C) number number 08-13-12 1540 J. Sumner Sonic Delivery 0.8,0.7 8207 120813 .01 TVA I SQN Outfall101 TN 08-13-12 1540 J. Sumner Sonic Delivery 0.9 8207 120813 .02 TVA/ SQN Intake TN SOP G4 -Exhibit G4.2, revision 01-03-12 Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled:

Sequoyah NP NPDES Number: TN0026450 Collected By: f) v) r/1). H." 4J t.r fvWir Field Identification I Sample Description SQN-101-TOX SQN-INT-TOX Grab/Comp. Comp Comp Relinquished By (Signature): BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of_l_ Environmental Testing Solution, Inc. 3 51 Depot Street. Phone: Fax: Asheville, NC 28801 828-350-9364 828-350-9368 Container Delivered By (Circle One): FedEx UPS Bus Client Other (specify): ______ _ General Comments: To{) k J 'i 11fJit-> o-f+ c; r 0 v+ A II 'I> 0 CiAo/ ::t';'\ fiO*kl-a Y C J 0. Collection Date/Time Number & Flow Volume (MGD) Rain Event? (Mark as Appropriate) Collected Start End No Trace '&""l"l'IJ. (P'7(J0 0 bOO t:,"t"' 2(2.5gal) X f-lt.t"' '}., Y-l)"'IJ-o 7 J-o -c:"t" o E.'t" 1(2.5 gal) Date/Time /0.,:;30 E-r Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. Whole Receipt Log Page J.:1,b *Sample temperature performed using Sample Receiving Thermometer: SN 6338 Date Time Received Received

Sample Project Sample Sample name and description State Comments Received Received by from Temp. (°C) number number 08-15-12 0945 K.Keenan Fed-Ex 1.3 8215 120815 .01 CORR ERI-AAF McQuay International NC 08-15-12 0945 K.Keenan Fed-Ex 0.7 8216 120815 .02 Statesville Analytical-Cleveland WWTP NC 08-15-12 0945 K.Keenan Fed-Ex 5.5 8217 120815 .03 United Water-Enfield WWTP NC 08-15-12 0945 K.Keenan Fed-Ex 5.5 8218 120815 .04 United Water-Scotland Neck WWTP NC 08-15-12 0945 K.Keenan Fed-Ex 0.3 8219 120815 .05 Craven County Wood Energy NC 08-15-12 0945 K.Keenan Fed-Ex 0.7 8220 120815 .06 Duke Energy-McGuire NS-Outfall 002 NC 08-15-12 0945 K.Keenan Fed-Ex 0.6 8221 120815 .07 Progress Energy-Shearon Harris E & E NC 08-15-12 0945 K.Keenan Fed-Ex 0.5 8222 120815 .08 Carolina Beach WWTP NC 08-15-12 0945 K.Keenan UPS 2.0 8223 120815 .09 Morehead City WWTP NC 08-15-12 1553 J. Sumner TVA Courier 1.9/1.9 8207 120815 .10 TVA-SQN -101 TN 08-15-12 1553 J. Sumner TVA Courier 2.0 8207 120815 .11 TV A-SQN -Intake TN SOP G4 -Exhibit G4.2, revision 01-03-12 Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled:

Sequoyah NP NPDES Number: TN0026450 Field Identification I Sample Description SQN-101-TOX SQN-INT-TOX Grab/Comp. Comp Comp Relinquished By (Signature): BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of_l_ Environmental Testing Solution, Inc. 3 51 Depot Street. Phone: Fax: Asheville, NC 28801 828-350-9364 828-350-9368 Container Delivered By (Circle One): FedEx UPS Bus Client Other (specify): ______ _ General Comments: hDk frl),...., o,.,fA/1 ctr fr0.,.-.., J&nf'<;/(t.. q f-o ( .3ot Number & Flow Volume (MGD) Collection Date/Time Rain Event? (Mark as Appropriate) Start -n-rJ.. ?-0 ?oo e.-t 0'10(90 E.T t-1(, -tJ-tl7{J £( Date/Time 0 * \\-\1.. Collected 2(2.5gal) 1(2.5 gal) If Yes, Inches II No Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. ............. Whole Effluer )ample Receipt Log Page llS *Sample temperature performed using Sample Receiving Thermometer: SN 6338 Date Time Received Received

Sample Project Sample Sample name and description State Comments Received Received by from Temp. {0C) number number 08-17-12 0950 K.Keenan Fed-Ex 0.6 8215 120817 .01 CORR ERI -AAF McQuay International NC 08-17-12 0950 K.Keenan Fed-Ex 0.6 8216 120817 .02 Statesville Analytical

-Cleveland WWTP NC 08-17-12 0950 K.Keenan Fed-Ex 2.0 8217 120817 .03 United Water-Enfield WWTP NC 08-17-12 0950 K.Keenan Fed-Ex 2.0 8218 120817 .04 United Water-Scotland Neck WWTP NC 08-17-12 0950 K.Keenan Fed-Ex 0.7 8219 120817 .05 Craven County Wood Energy NC 08-17-12 1130 J. Sumner Murphy Courier 0.8 8226 120817 .06 Duke Energy-McGuire NS-Upper Composite NC 08-17-12 1130 J. Sumner Murphy Courier 0.8 8226 120817 .07 Duke Energy-McGuire NS-Lower Composite NC 08-17-12 1338 J. Sumner TVA Courier 2.2/2.2 8207 120817 .08 TVA-SQN-Outfall101 TN 08-17-12 1338 J. Sumner TVA Courier 2.4 8207 120817 .09 TVA-SQN -Intake TN SOP G4 -Exhibit G4.2, revision 01-03-12 ll s Page 1 of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Seguoyah Nuclear Plant NPDES #: TN0020168 Project#: ____ _ Dilution preparation information: Dilution prep (%) 10.8 21.6 Effluent volume (mL) 270 540 Diluent volume (mL) 2230 1960 43.2 1080 1420 Total volume (mL) 2500 2500 2500 Test organism information: 86.4 2160 340 2500 Organism age: 1.o 0\-b Date and times organisms \'\*\'1.. \\o()() *. were born between: 100 2500 0 2500 Organism source: Pe Transfer bowl information: pH= '*\.DO s.u. Temperature = 1.S. \ oc Average transfer volume: o. County: Rhea Outfall: 101 Comments: Test information: Randomizing template:

Incubator ntunber and shelf location:

Artemia CHM number: "ec..to\A) t.R"' \Qt;l.. Drying information for weight determination: Date I Time in oven: o&* '2.\* n .. Initial oven temperature: lob' c.. Date I Time out of oven: Final oven temperature: *c.. Total drying time: 1-\l*Jo\wtS. \-..00 Daily feeding and renewal information: Day Date Morning feeding 5 6 7 Afternoon feeding Test initiation,

renewal, or termination Sample numbers used MHSW batch used SOP A T20 -Exhibit AT20 .3, revision 07-01-12 s Page2 of6 Species:

Pimephales promelas Client: TV A I Seguoyah Nuclear Plant, OutfalllOl, Non-treated Date: 0&* I q

l ,_ Survival and Growth Data Day t;U.NT.KUL 21.6% A B c D E F G H I J 0 /0 f() 'D /Q /0 /0 IC 10 /0 10 1 /0 /Q 10 10 /Q 10 10 It) IO 10 2 /0 to IO 10 10 /() lO /0 10 IO 3 }0 /0 {(J 10 10 IC IO 10 10 1\) 4 10 /0 lO IO It> 10 10 10 tD IQ 5 10 10 10 10 10 10 10 10 IC /() 6 /0 IC 10 ID ID IC 10 IQ /C 10 7 /O ID /0 10 /Q 10 tD I(J 10 10 A= Pan weight (mg) " Tray color code:: \1?\..\-'P:.,.,k t4.4-l 14.1o t4.zs Analyst:

M._ t3.ss t4.z4 13.24 13.Sc. 13,\.8 '3.3S IZ.!1S Date: QS*aC.*'2 B = Pan + Larvae weight (mg) Analyst: 't,C).'30 \'1.7 3 z.o.l' Date: IS.6S" l't.'l5 zo.ss -zn.S'i ,q.t7 1.0.3'3 C = Larvae weight (mg) = B-A Hand calculated. A._ C..\S s."\"\ ... '\ ... "\4\ ... G& S .. Analyst: Weight per initial number oflarvae (mg) ' = C I Initial number of larvae 'Q'-r>>"' -b f'\1 J' Hand calculated. )l[...,... ct ... \o c; " t..; y "" "-Analyst: o* o* o' 0" t:J. o* o* t)" ()' '-Average weight per Percent reduction o.s&'-t 0. -&, 7. initial number of from control (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk = sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: K L 10 ID IO 10 to to 10 10 10 ID I(J 10 10 10 /Q 10 IS.C-) 2.1 .1'3 "ZJ .8.3 Co.1.Co AI '0 t:l f;J ... .1. 7, SOP AT20-Exhibit AT20.3, revision 07-01-12 ' ,.-**;>. !ET Page 3 of6 Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant, OutfalllOl, Non-treated Date: o&* t'-\.*1'-- Survival and Growth Data Day 43.2% 86.4% 100% M N 0 p .Q R s T u v w X 0 I() 10 to /0 IC /() 10 I() /D /0 /(:) IO 1 10 10 10 10 10 IO 10 10 10 to 10 I() 2 ID 10 10 IO to lD JO 10 /() 10 /() 10 3 (tJ 10 10 /0 /Q 10 10 10 I(;) to tO /(J 4 ({J IQ /Q IO /0 IO /Q ID /0 IO IO co 5 /D /() 10 10 10 10 10 10 10 ID 10 I() 6 /tl to ID 10 IC ((J /\) I{) It) /Q /Q 10 7 /C lb 1.-Y'\ 10 IC /0 10 IO t(J lb /() 10 IO A = Pan weight (mg) "' Tray color code:: 'P:l\1.. lZ..tA r4:5o Analyst: _t_ \3.So *'3.c-o

4.3, S.S1 3,:}-t\

t'3.c;o t3.1o 1:!..'31 Date: B = Pan + Larvae weight (mg) Analyst: 1Cf.'5l. 11.1.3 ,q_,-z. z.o.'"IS IS."7S 11-'fZ l't. 'fZ. 'Z.I.'I'2. -zo.'f'J "'Z..O. Z.l Date: Otl*1:7*1't. C = Larvae weight (mg) = B -A Hand s.&1.. '-\ .. &1 t..l'-\. Co. l'-\ s.c.:!t \..4\0 s.ofo 5.1\ Analyst: Weight per initial number oflarvae (mg) ' = C I Initial number of larvae Hand calculated. A 4V ,., 9,., A'? , r;f \0 'C) "0 ':> 'D y o* Analyst: .,...._ ... o* '0'" t:1 tl. o' cr o* o* b. ' \. ' t::l Average weight per Percent reduction o.So1. O.bOio initial number of from control (%) , 4.47. Iarvae_im2) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: SOP AT20-Exhibit AT20.3, revision iET Envln>nrn<ntaiTestlngSolutions,lnc. Page 4 of 6 Day 0 1 2 3 4 5 6 7 A= Pan weight (mg) Tray color code:: £ale. Analyst: __ Date: DS*Ol .. *\1. B = Pan + Larvae weight (mg) Analyst: OU Date: Oi*z.?.it. C = Larvae weight (mg) = B -A ' Hand calculated. Y Analyst: g""""'_,___ Weight per initial number oflarvae (mg) = C I Initial number of larvae Hand calculated.A{ Analyst: (:;;J-_ _.. __ Average weight per Percent reduction initial number of from control(%) larvae 10 tO lO /0 /0 (0 I() IO 10 10 /0 10 10 10 to ft) 10 ID t(J 13.84 14.19 14.tt 14.24 ttt.Z.l 1.0."17 1.l. -z.l." S"!t1 c&,\.C\ tc..t1 Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: SOP AT20 -Exhibit AT20.3, revision 07-01-12

*
Environmental Testing Solutions, Inc. Concentration(%)

Rep6cate A Control B c D E 10.8% F G H I 21.6% J K L M 43.2% N 0 p Q 86.4% R s T u 100% v w X y 100% Intake z AA BB Outfall101: Dunnett's MSD value: PMSD: Intake: Dunnett's MSD.value: PMSD: Initial number of larvae IO IO 10 IO 10 10 IO IO IO 10 IO 10 IO 10 IO IO IO IO IO IO 10 IO IO IO IO IO IO IO 0.0994 17.0 0.0726 12.4 Final number of larvae IO IO 10 IO 10 10 IO IO IO 10 10 10 IO 10 IO 10 IO 10 10 IO 10 IO IO IO 8 IO IO 10 A= Pan weight (mg) I4.47 13.58 I4.24 13.24 14.10 13.86 13.68 13.38 I4.25 I2.95 15.67 14.97 13.50 13.60 I4.75 14.3I I2.64 13.87 13.79 I4.52 13.50 I3.70 13.37 I4.50 13.84 I4.78 I4.71 I4.24 MSD= PMSD= TVA I Sequoyah Nuclear Plant, Outfall101 Non-treated August 14-21, 2012 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Not for Compliance Assessment, Internal Laboratory QC B =Pan+ Larvae Larvae weight (mg) Weight I Surviving weight(mg) =A-B number oflarvae (mg) 20.30 5.83 0.583 I9.73 6.15 0.6I5 20.I6 5.92 0.592 18.68 5.44 0.544 19.45 5.35 0.535 20.55 6.69 0.669 20.54 6.86 0.686 19.87 6.49 0.649 20.33 6.08 0.608 18.54 5.59 0.559 21.93 6.26 0.626 21.83 6.86 0.686 I9.32 5.82 0.582 19.23 5.63 0.563 19.62 4.87 0.487 20.45 6.14 0.614 I8.78 6.I4 0.6I4 I9.42 5.55 0.555 19.42 5.63 0.563 21.42 6.90 0.690 18.82 5.32 0.532 20.43 6.73 0.673 I8.43 5.06 0.506 20.2I 5.7I 0.57I I9.21 5.37 0.67I 20.47 5.69 0.569 20.22 5.5I 0.55I 21.11 6.87 0.687 Minimum Significant Difference Percent Minimum Significant Difference Mean weight I Coefficient of variation Surviving number of (Meanweightprrsurviring larvae(mg) numbororlarva*)(%) 0.584 5.1 0.635 10.7 0.620 8.5 0.562 9.6 0.606 I0.3 0.571 I2.9 0.620 Il.2 Weight I Initial number of larvae (mg) 0.583 0.615 0.592 0.544 0.535 0.669 0.686 0.649 0.608 0.559 0.626 0.686 0.582 0.563 0.487 0.6I4 0.6I4 0.555 0.563 0.690 0.532 0.673 0.506 0.57I 0.537 0.569 0.55I 0.687 Project number: 8207 Mean survival Mean weight I Initial Coefficient of Percent reduction from (%) number of larvae variation (Mean weight control(%) (mg) perinitialnumberoriarvae) (%l_ 100.0 0.584 5.1 Not applicable 100.0 0.635 10.7 -8.8 100.0 0.620 8.5 -6.2 100.0 0.562 9.6 3.8 100.0 0.606 10.3 -3.8 100.0 0.571 12.9 2.2 95.0 0.586 11.7 -0.4 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (I Oth percentile) = I2%. Upper PMSD bound determined by USEP A (90th percentile) = 30%. Lower and upper PMSD bounds were determined from the I Oth and 90th percentile, respectively, of PMSD data from EPA's WET InterlaboratOiy Variability Study (USEP A, 2001 a; USEP A, 200 I b). USEPA. 200Ia, 200Ib. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-82I-B-OI-004 and EPA-821-B-OI-005. US Environmental Protection Agency, Cincinnati, OH. File: sqn101_081412data.xlsx Entered by: J. Sumner Reviewed by:--? Start Date: 8/14/2012 End Date: 8/21/2012 Sample Date: August 2012 Comments: Non-treated Cone-% 1 D-Control 0.5830 10.8 0.5350 21.6 0.6080 43.2 0.5820 86.4 0.6140 100 0.5320 Intake 0.5370 Cone-% Mean D-Control 0.5835 10.8 0.6348 21.6 0.6198 43.2 0.5615 86.4 0.6055 100 0.5705 Intake 0.5860 Auxilia!1 Tests 2 0.6150 0.6690 0.5590 0.5630 0.5550 0.6730 0.5690 N-Mean 1.0000 1.0878 1.0621 0.9623 1.0377 0.9777 1.0043 TVA I Sequoyah Nuclear Plant, Outfall101 Non-treated August 14-21, 2012 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Test ID: PpFRCR Sample ID: TVA I SQN 101 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas 3 4 0.5920 0.5440 0.6860 0.6490 0.6260 0.6860 0.4870 0.6140 0.5630 0.6900 0.5060 0.5710 0.5510 0.6870 Transform: Untransformed 1-Tailed Isotonic Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean 0.5835 0.5440 0.6150 5.069 4 0.6127 1.0000 0.6348 0.5350 0.6860 10.744 4 -1.242 2.410 0.0994 0.6127 1.0000 0.6198 0.5590 0.6860 8.465 4 -0.879 2.410 0.0994 0.6127 1.0000 0.5615 0.4870 0.6140 9.607 4 0.533 2.410 0.0994 0.5835 0.9524 0.6055 0.5550 0.6900 10.256 4 -0.533 2.410 0.0994 0.5835 0.9524 0.5705 0.5060 0.6730 12.860 4 0.315 2.410 0.0994 0.5705 0.9312 0.5860 0.5370 0.6870 11.706 4 Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98195 0.884 0.03805 -0.4693 Bartlett's Test indicates egual variances {[! = 0.82l 2.21405 15.0863 Test {1-tail, 0.051 Dunnett's Test Treatments vs D-Control Point IC05 IC10 IC15 IC20 IC25 IC40 IC50 % 87.934 >100 >100 >100 >100 >100 >100 0.8 0.7 0.6 l 0.5 e (!) 0.4 0.3 0.2 0.1 0 :g c: 0 u 0 so CX! 0 NOEC LOEC ChV TU MSDu MSB MSE F-Prob 100 >100 1 0.09943 0.17041 0.00332 0.0034 0.4589 Linear Interpolation (200 Resamples) 95% Skew <<:! N N (I) v 1.0 -r-------------, Q) 0.9 0.8 0.7 0.6 1!1 0.5 &. 0.4 0.3 0.2 Q1

- - - ,*' -0.1 *,.' -0.2 +-r--r--.--.-..,.-.,......,r-r--.--r--r-r-r--r-1 0 50 100 150 Dose% Dose-Response Plot '<I: 0 CD IX) .e! .19 .E 1-tail, 0.05 level of significance df 5, 18 File: sqn101_081412data.xlsx Entered by: J. Sumner Reviewed by:-+

Start Date: 8/14/2012 End Date: 8/21/2012 Sample Date: August 2012 Comments: Non-treated Cone-% 1 D-Control 0.5830 10.8 0.5350 21.6 0.6080 43.2 0.5820 86.4 0.6140 100 0.5320 Intake 0.5370 Cone-% Mean D-Control 0.5835 10.8 0.6348 21.6 0.6198 . 43.2 0.5615 86.4 0.6055 100 0.5705 Intake 0.5860 Auxiliary Tests 2 0.6150 0.6690 0.5590 0.5630 0.5550 0.6730 0.5690 N-Mean 1.0000 1.0878 1.0621 0.9623 1.0377 0.9777 1.0043 TVA I Sequoyah Nuclear Plant, Intake Non-treated August 14-21, 2012 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Test ID: PpFRCR Sample ID: TVA I SQN 101 -Intake Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas 3 0.5920 0.6860 0.6260 0.4870 0.5630 0.5060 0.5510 Mean 0.5835 0.6348 0.6198 0.5615 0.6055 0.5705 0.5860 4 0.5440 0.6490 0.6860 0.6140 0.6900 0.5710 0.6870 Transform: Untransformed Min Max CV% 0.5440 0.6150 5.069 0.5350 0.6860 10.744 0.5590 0.6860 8.465 0.4870 0.6140 9.607 0.5550 0.6900 10.256 0.5060 0.6730 12.860 0.5370 0.6870 11.706 1-Tailed N t-Stat Critical MSD 4 4 4 4 4 4 4 -0.067 1.943 0.0726 Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) F-Test indicates egual variances (p = 0.20) 0.88547 5.37752 0.749 47.4683 1.36005 1.98359 Hypothesis Test (1-tail, 0.05) Homoscedastic t Test indicates no significant differences Treatments vs D-Control 0.7 0.6 J 0.5 0 (5 0.4 i;' c 0.3 "' 0.2 0.1 co 0 ,.. MSDu MSDp MSB 0.07258 0.12439 1.2E-05 Dose-Response Plot oo:r: <0 co 0 0 ,.. 1-tail, 0.05 level of significance MSE F-Prob df 0.00279 0.94881 1' 6 File: sqnl01_081412data.xlsx Entered by: J. Sumner Reviewed by: T I I I li li li r. li Species: Pimeohales promelas Date: __ o=-t_*l_'-....;;..l*_.;\_.;1...=--- Ciient: TV A I Seguoyab Nuclear Plant. OutfalllOl. Non-treated CONTROL Non-treated 10.8% 21.6% 43.2% 86.4% 100% 100% Intake Page 5 of6 *Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet SOP AT20-Exhibit AT20.3, revision 07-01-12 1-I I I I I I tration CONTROL 100% 100% Intake s Page 6 of6 Analyst Parameter

Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet SOP AT20-Exhibit AT20.3, revision 07-01-12 Concentration Control 10.8% 21.6% 43.2% 86.4% 100% 100% Intake TV A I Sequoyah Nuclear Plant, Outfa11101

-Non-treated August 14-21, 2012 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number: ______ 82......;0_7 File: sqn1 01_081412chem.xls Entered by: J. Sumner Reviewed by:+ !ETS 0 Environmental Testing Solutions, Inc. Page 1 of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: Tennessee Valley Authority Facility: Seguoyah Nuclear Plant NPDES #: TN0026450 Project#: --=4-=-1...=<0=------- Dilution preparation information: Dilution prep(%) 10.8 21.6 Effluent volume (mL) 270 540 Diluent volume (mL) 2230 1960 43.2 1080 1420 Total volume (mL) 2500 2500 2500 Test organism source information: Organism age: < 24-hours old 86.4 100 2160 2500 340 0 2500 2500 Date and times organisms were born Ol*,"\* \1... 0'-LO "tt ct\lt) between: Culture board: ot*cn-,,_ Replicate number: I I 2 I 3 I 4 I 5 I 6 I 7 I 8 I 9 10 Culture board cup number: \1.1 "U> 1-a.\ 1\'L 11-t I lll "s Transfer vessel information: I pH= ., * ., \ s.u. Temperature = "1.'{:" oc Average transfer volume (mL): I 0

0'!.1.1.....Q Daily renewal information:

County: Hamilton Outfall: 101 Comments: Test information: Randomizing template color: Incubator number and shelf location: YWTbatch: Selenastrum batch: Day Date Test initiation and feeding, renewal and feeding, or termination time MHSW batch used Samplen Outfall lOt 0 2 3 4 5 6 7 6-()'-,.t) 'Z.e,1... o&*()t*l"'\.. ... Analyst Control information: Summary of test endpoints: Control-I Control-2 Acceptance criteria % of Male Adults: 01. _0_7, 7-day LCso )J0()7. %Adults having 3ra Broods: /007 1007. ;?!80% NOEC 1007. % Mortality: C7 .. 0_7. LOEC )1007. Mean Offspring/Female: 3l. '2..

?! 15.0 offspring/female ChV )1007 %CV: '-* '1.7., s. "7. <40.0% IC2s ., 1001. SOP AT11-Exhibit AT11.2, revision 07-01-12 f I; I I I I Page 2 of7 Species:

Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant. Outfall101 Date: 0&

1'-t*l"'l.-

CONTROL-1 Survival and Reproduction Data Replicate number Day* 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 () 0 0 D b 0 a Adult mortality '-L L-L L L L L L L 2 Young produced D. D a a () 0 (') 0 0 0 Adult mortality '-\..... \...... \..._ '--L \.._ L L. \._. 3 Young produced C) 0 0 0 () 0 c a 0 () Adult mortality L '-'--'--'-'---'-L '-'---4 Young produced __ c; '4 s s 5 5 Co \.. Adult mortality \..... '--'--L.,_ '--\..._ L '--'--'--*5 Young produced ' \ \.\ I() \1.,... \C \1.... \\ \\ \_\ Adult mortality L \...... '--'--'---L L \..._ '--L... 6 Young produced D b 0 0 a 0 C> 0 0 0 Adult mortality L '-\....... L \..._ '-L.... L L \.._ 7 Young produced 1"\ -'a* &5 Lt. *'-\ ,, \S Total young produced { eJAj !if "l.'\ 7..i l>>b --,v Final Adult Mortality '-"-"-'--. '--'-'--\...... L. X for 3rd Broods " ')(... .. ::>5-_25-,. }C.. )£. )<-Note: Adult mortahty (L-hve, D-dead), SB-spht brood (smgle brood split between two days), c_.o =carry over (offspnng earned over With adult dunng transfer). Concentration: % Mortali *: 7 .. ,,_ CONC: 10.8% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced L) () _0 0_ Q_ 0 0 0 0 _(') Adult mortality L L L L L L.. L L L l_ 2 Young produced 0 0 a 0 0 Q D {) 0 _D Adult mortality \._. L L L L L.. L L L.... L.. 3 Young produced a 0 0 0 0 0 a_ 0 0 0 Adult mortality L \. L L L L L L L L. 4 Young produced *c. 4 \.{ to y (., 6 _S Adult mortality L. \...-L.. '--\.._ \.._ '--\.._ \...._. L 5 Young produced \L.. \\ ,,_ \"'L \0 '1... )(.. -Adult mortality \..... '--L \...._ L L L L.. '--\...._. 6 Young produced 0 0 CJ 0 0 0 u 0 0 0 Adult mortality \._ L L-L L L '--L L \.....__ 7 Young produced \Q ,..., \i li ,, \\ \\o I'-\ lS Total young produced

:!JS 3.'1 3'! Final Adult Mortality

\...... '-\...._ \. L. L L. L L.. \..._ Note: Adult mortality (L =live, D-dead), SB-split brood (single brood split between two days), CO= can over( offspring carried over with adult during transfer). Concentration: % Mortality:

07. Mean Offspring/Female:

%Reduction from Control-I: -K-07. SOP ATll-Exhibit AT11.2, revision 07-01-12 1'"*'*_**,*****_*.'.*_*.* .. *"* *. _._ .. :.*_*. ,_"'> I I I ; lETS 6 Environmental Testing Sclutlons.lnc. Page 3 of7 Species: Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant. Outfall 101 Date: 08*J\f--I 1-CONC: 21.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced a 0 0 0 0 0 0 0 0 c Adult mortality L. '-\_.. '---L '--L. '--L. \...... 2 Young produced 0 0 0 a 0 0 0 0 0 Q Adult mortality '-\._ '-L L \_.. L L. L .__ 3 Young produced C) 0 b b 0 0 0 0 0 0 Adult mortality L L L. L..... \.._ '--L L_ '--4 Young produced $ s 5 "-' s 5 -q '-\ q Adult mortality '-'-'---\.._ '--'--\.... L.. L.. \..._ 5 Young produced \1;:) \\ \"l-\0 \C \'S \\ Adult mortality '-\........ \...... \...... \.._ L.. \...._ \...... \....... '-' 6 Young produced D 0 0 0 0 0 0 TI 0 0 Adult mortality L '-L L.. \...._ '--\._ \...... \......_ L-7 Young produced a*& \S \\o \\.. "" \'\ l-'\ \\. ,., Total young produced o\o. "!:.\ '!\ ob :!.c; 31-'!.0 ., Final Adult Mortality L '--\....... '--'-\...... '-'--\....,. \....... Note: Adult mortality (L =live, D =dead), SB -split brood (single brood split between two days), CO-can ovei7offsorimt carried over with adult durin2 transfer). Concentration: "' % Mortality:

01. Mean Offspring/Female:

32.R %Reduction from Control-I:

s. \7. CONC: 43.2% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 (') 0 D 0 a D C) 0 0 Adult mortality

'-\..... L L L-L-L L-L L. 2 Young produced C) 0 D 0 0 0 C) 0 6 0 Adult mortality L L L \._ L L L L L L 3 Young produced a 0 0 0 6 .t'J 0 0 {) Adult mortality L '-'-L L L L L L. L. 4 Young produced \, s s -s l.o s '-1' s Adult mortality L \._.. \.... L. L L L L \.._ '--5 Young produced \L \1,.. l1.. \\ \\ -'"' IL.. 11.. Adult mortality \._ '-.. L L L '-L L L L.. 6 Young produced 0 0 0 0 0 0 a a a Adult mQrtality '--L \ \... l -L... L L L '-7 Young produced \& ,., \i \t\ *s l9 Total young produced 1.'-\ 3'\ 3\ 3\ !a\ 31 3'-l Final Adult Mortality \..... '-'-\..._ '---L \.... L \.... \...... Note: Adult mortality (L =live, D =dead), SB-split brood (single brood split between two days), CO_, car overloffsorin2 carried over with adult durin2 transfer). Concentration: %Mortality:

07. Mean Offspring/Female:

33.1 % Reduction from Control-!: SOP A Til-Exhibit AT11.2, revision 07-01-12 !ETS 0 EnvlronmentaiTesttngSolutlonr,lnc. Page4 of7 Species: Ceriodaphnia dubia Client: TV A I Seguoyah Nuclear Plant. OutfalllOl Date: __ CONC: 86.4% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced (J 0 0 0 0 0 0 D 0 0 Adult mortality L-\.... '-L.. L-L-L L-L L 2 Young produced D 0 a () () 0 0 0 CJ 0 Adult mortality '--'--L L L '-L L L L-3 Young produced 0 0 D 0 0 D 0 c *o C) Adult mortality \.... '--L L '--L '--L L.. L_ 4 Young produced s , 5 $ '"' s '-s \.. c. Adult mortality L-L. L. L... '-\....... \._ L \...... L 5 Young produced \"'l-\ "'L. \\ \ .,_ 11.. \L \?, Adult mortality L \...... '-\........ '--L '--L... '-\...... 6 Young produced C\ 0 b D 0 0 e 6 0 () Adult mortality '--'-'-'-\._ L-L... L._ '--\....-7 Young produced \'-\S \, \ \.4 ,, ,,. Total young produced ?l-\ !.\o !.5 .3'\ 3.\ Fi.nal Adult Mortality L '-'--\..... \..... L "'-'-'--'---Note: Adult mortality (L -live, D =dead), SB =split brood (single brood split between two days), CO= can over (offspring carried over with adult during transfer). Concentration: %Mortality: ()7. # Mean Offspring/Female: %Reduction from Control-1: -n. S7. CONC: 100% Survival and Reproduction Data Replicate number

Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 D a c 0 0 0 Adult mortality L l_. L L L L L-L. L-L 2 Young produced D 0 0 0 0 0 0 a 0 0 Adult mortality L-L.. L.. L.. \..._ L L \...... L. L.... 3 Young produced 0 a tJ 0 0 () a 0 () a Adult mortality L '--L L L. L '-L. \..._. L 4 Young produced , .s "' 5 s s Co t, Adult mortality L '--L... L* L L L L L L 5 Young produced l'-\ ,,_ lb ,,_ \""L \'-\ \'b Adult mortality

\.... \._ L L L \._ L.. L. L L 6 Young produced () 0 0 0 0 0 0 0 0 (). Adult '-L L L L L. L L L L_ 7 Young produced \& \' 1(. ., ,, "2..0 1& 11 Total young produced 3ft .31 31 3'--l 34 aS 31 38 Final Adult Mortality '-\... \.._ \.... L. L L. L. \..___ *'--... Note: Adult mortality (L =live, D =dead), SB =split brood (single brood split between two days), CO= can over (offSpring carried over with adult during transfer). Concentration: %Mortality: C7. ;: I Mean Offspring/Female: %Reduction from Control-1: -.1.07. I SOP A Til-Exhibit AT11.2, revision 07-01-12 Page 5 of7 Species: Ceriodaphnia dubia Client: TV A I Seguoyah Nuclear Plant, OutfalllOl Date: __ o_A_*_l_'-l_*_l'"L ___ _ CONTROL-2 Survival and Reproduction Data Replicate number Day. 1 2 3 4 5 6 7 8 9 10 1 Young produced (") c () 0 0 a a 0 0 0 Adult mortality '-L. L L. '--L L-\..... L-L.. 2 Young produced 0 0 0 0 0 0 0 0 (} 0 Adult mortality L \.....-L L. '--'--L. '--L_ 3 Young produced D D 0 D D a 0 0 0 a Adult mortality \.. -'--\.._ \.._ 'L \ L L L L 4 Young produced '-\ s s \o '-( 4 4 4 .'-\ Adult mortality L. \.....-'-'--'--\.._. '--'-'--\....... 5 Young produced \0 \'"Z... \b ,,_ \0 \0 \C \\ L 1\ **:******

. Adult mortality

'--L '--\.._ \....... L.. '-L '-"--6 Young produced D 0 a a 0 0 0 .c 0 a Adult mortality \.._.. \._ '--L \.._ '-\.-L '-L.. 7 Young produced 1\. \i \\o \ \..\ \S \4 \U. \S Total young produced "'S:) :!:>\ '3\ Final Adult Mortality '-'--'-'--\..... '-'-"-"--X for 3rd Broods 'X-><-X >"" X . . Note: Adult mortality (L -live, D-dead), SB-spht brood (smgle brood split between two days), carry over (offspnng earned over With adult dunng transfer) . coNe: 100% Intake Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L. L L L L L-L L-L-L 2 Young produced 0 0 0 0 0 0 0 0 0 () Adult mortality '--'--L L L-L-'---\....... \._.. L. 3 Young produced {) 0 D D 0 0 0 0 0 0 Adult mortality L L L-L.. L L.. L L 'L L. 4 Young produced (., \o s s ., s s 4 ._ Adult mortality \.... '-'\...._ '--\._ L.. '--\.._ L L 5 Young produced \\ ,,__ \'1.. lb '"\ \1... t"l.. ' \ Adult mortality '-\...... '-L. L.. L. '--L L... 6 Young produced 0 () a 0 0 0 a _Q 0 [) Adult mortality L L \._ L L L L L L L 7 Young produced \\o \1::> \S \& \lo '" \"'"\ Total young produced "1'\ 3to 32 3fo 3'\ Final Adult Mortality '-\..... '-'--'--'--'--\...... L. '--Note: Adult mortality (L -live, D =dead), SB-split brood (single brood split between two days), CO= can over (offsprin2 carried over with adult durin2 transfer). Concentration: %Mortality:

07. Mean Offspring/Female:

%Reduction from Control-2: -f.,. I 7! SOP AT11-Exhibit AT11.2, revision 07-01-12 Control-1 Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 5 4 4 5 11 11 10 6 0 0 0 7 14 18 15 Total 30 33 29 10.8% Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 5 6 4 5 13 12 11 6 0 0 0 7 18 16 18 Total 36 34 33 Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 5 5 5 5 13 11 12 6 0 0 0 7 18 15 16 Total 36 31 33 43.2°/o Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 6 5 5 5 13 12 12 6 0 0 0 7 18 17 17 Total 37 34 34 TVA I Sequoyah Nuclear Plant, OutfalllOl-Non-treated August 14-21, 2012 Verification of Ceriodaphnia Reproduction Totals Re!llicate number Total 4 5 6 7 8 9 10 Day Replicate number 1 2 3 4 5 6 7 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 5 5 5 5 6 4 6 49 4 5 7 5 5 4 5 6 12 10 9 12 11 11 11 108 5 13 12 12 11 12 12 14 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 16 16 14 17 15 15 15 155 7 16 15 19 18 19 16 14 33 31 28 34 32 30 32 312 Total 34 34 36 34 35 33 34 100% Replicate number Total 4 5 6 7 8 9 10 Day Replicate number 1 2 3 4 5 6 7 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 6 6 4 6 5 5 51 4 6 7 5 6 5 5 5 12 12 10 13 12 12 12 119 5 14 12 13 12 12 14 13 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 17 17 19 17 16 14 15 167 7 18 18 19 16 15 19 17 33 35 35 34 34 31 32 337 Total 38 37 37 34 32 38 35 Control-2 Replicate number Total 4 5 6 7 8 9 10 Day Replicate number 1 2 3 4 5 6 7 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 5 6 5 5 4 4 4 48 4 4 5 5 5 6 4 4 10 10 13 13 13 11 13 119 5 10 12 13 12 10 10 10 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 16 14 19 17 16 17 13 161 7 16 17 16 14 16 15 18 31 30 37 35 33 32 30 328 Total 30 34 34 31 32 29 32 100% Intake Replicate number Total 4 5 6 7 8 9 10 Day Replicate number 1 2 3 4 5 6 7 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 5 5 6 5 4 5 50 4 6 4 6 5 5 7 5 12 11 11 12 14 12 12 121 5 11 12 12 13 10 14 12 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 15 15 15 19 15 18 18 167 7 16 13 15 15 18 15 16 31 31 31 37 34 34 35 338 Total 33 29 33 33 33 36 33 8 9 0 0 0 0 0 0 5 6 12 13 0 0 18 17 35 36 8 9 0 0 0 0 0 0 6 6 13 13 0 0 20 18 39 37 8 9 0 0 0 0 0 0 4 4 13 11 0 0 14 14 31 29 8 9 0 0 0 0 0 0 5 4 12 13 0 0 15 19 32 36 10 0 0 0 6 13 0 18 37 10 0 0 0 6 13 0 19 38 10 0 0 0 4 12 0 15 31 10 0 0 0 6 11 0 17 34 Total 0 0 0 54 124 0 170 348 Total 0 0 0 57 129 0 179 365 Total 0 0 0 45 113 0 155 313 Total 0 0 0 53 120 0 159 332 File: sqn101_081412data.xlsx Entered by: J. Sumner Reviewed by: 4---- Concentration (%) 1 2 3 Control-1 30 33 29 10.8% 36 34 33 21.6% 36 31 33 43.2% 37 34 34 86.4% 34 34 36 100% 38 37 37 Control-2 30 34 34 100% Intake 33 29 33 Outfall101: Dunnett's MSD value: 2.021 PMSD: 6.5 Intake: Dunnett's MSD value: 1.459 PMSD: 4.7 TV A I Sequoyah Nuclear Plant, Outfall101 Non-treated August 14-21, 2012 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: ___________ 8_2_0_7 Replicate number Survival Average reproduction Coefficient of Percent reduction from (%) (offspring/female) variation (%) control (%) 4 5 6 7 8 9 10 33 31 28 34 32 30 32 100 31.2 6.2 Not applicable 33 35 35 34 34 31 32 100 33.7 4.4 -8.0 31 30 37 35 33 32 30 100 32.8 7.6 -5.1 31 31 31 37 34 34 35 100 33.8 6.7 -8.3 34 35 33 34 35 36 37 100 34.8 3.5 -11.5 34 32 38 35 39 37 38 100 36.5 6.0 -17.0 31 32 29 32 31 29 31 100 31.3 5.6 Not applicable 33 33 36 33 32 36 34 100 33.2 6.0 -6.1 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effiuent toxicity test. LowerPMSD bound determined byUSEPA (lOth percentile)

13%. Upper PMSD bound determined by USEPA (90th percentile)

47%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200la; USEPA, 2001b). USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effiuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200la, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effiuent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: sqn101_081412data.xlsx Table populated from associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheet. Spreadsheet entered by: J. Reviewed by: Start Date: 8/14/2012 TVA I Sequoyah Nuclear Plant, Outfall101 Non-treated August 14-21, 2012 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Test ID: CdFRCR Sample ID: TVA/SQN 101 End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: Comments: Non-treated Cone-% 1 2 3 4 5 6 7 8 Control-1 30.000 33.000 29.000 33.000 31.000 28.000 34.000 32.000 Control-2 30.000 34.000 34.000 31.000 32.000 29.000 32.000 31.000 10.8 36.000 34.000 33.000 33.000 35.000 35.000 34.000 34.000 21.6 36.000 31.000 33.000 31.000 30.000 37.000 35.000 33.000 43.2 37.000 34.000 34.000 31.000 31.000 31.000 37.000 34.000 86.4 34.000 34.000 36.000 34.000 35.000 33.000 34.000 35.000 100 38.000 37.000 37.000 34.000 32.000 38.000 35.000 39.000 Intake 33.000 29.000 33.000 33.000 33.000 36.000 33.000 32.000 Transform: Untransformed Rank Cone-% Mean N-Mean Mean Min Max CV% N Sum Control-1 31.200 0.9968 31.200 28.000 34.000 6.193 10 Control-2 31.300 1.0000 31.300 29.000 34.000 5.645 10 10.8 33.700 1.0767 33.700 31.000 36.000 4.435 10 140.00 21.6 32.800 1.0479 32.800 30.000 37.000 7.578 10 122.00 43.2 33.800 1.0799 33.800 31.000 37.000 6.660 10 136.50 86.4 34.800 1.1118 34.800 33.000 37.000 3.532 10 151.00 100 36.500 1.1661 36.500 32.000 39.000 5.954 10 150.50 Intake 33.200 1.0607 33.200 29.000 36.000 5.991 10 Tests Statistic Kolmogorov D Test indicates non-normal distribution (p <= 0.05) 0.98421 Bartlett's Test indicates equal variances (p = 0.35) 5.58769 The control means are not sianificantl:z:: different{!;!= 0.91} 0.12078 Test {1-tall, NOEC LOEC ChV TU Steel's Many-One Rank Test 100 >100 1 Treatments vs Control-1 Linear Interpolation (200 Resamples) Point IC05 IC10 IC15 IC20 IC25 IC40 IC50 % SD >100 >100 >100 >100 >100 >100 >100 95%CL Skew 1.0 0.9 0.8 0.7 0.6 Ql 0.5 Ill c 0.4 0 i 0.3 tt:: 0.2 0.1 0.0 CD-Ceriodaphnia dubia 9 10 30.000 32.000 29.000 31.000 31.000 32.000 32.000 30.000 34.000 35.000 36.000 37.000 37.000 38.000 36.000 34.000 1-Tailed Isotonic Critical Mean N-Mean 33.800 1.0000 75.00 33.800 1.0000 75.00 33.800 1.0000 75.00 33.800 1.0000 75.00 33.800 1.0000 75.00 33.800 1.0000 Critical Skew Kurt 0.895 -0.0927 -0.4597 15.0863 2.10092 -0.1 '"' .......... .. 45 40 35 0 30 25 "'0 20 Cll tt:: 15 10 5 0 g 1! c: c: 8 8 -0.2 -0.3 Dose-Response Plot CXl lq C'f '<1: N (') co ...,. Cl) 0 50 Dose% 100 150 File: sqnl01_081412dataxlsx Entered by: J. Srer Reviewed TV A I Sequoyah Nuclear Plant, Outfall101 Non-treated August 14-21, 2012 Statistical Analyses Analysis used for PMSD calculation only. Survival and Start Date: 8/14/2012 Test ID: CdFRCR Sample ID: End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: Comments: Non-treated Cone-% 1 2 3 4 5 6 7 8 Control-1 30.000 33.000 29.000 33.000 31.000 28.000 34.000 32.000 Control-2 30.000 34.000 34.000 31.000 32.000 29.000 32.000 31.000 10.8 36.000 34.000 33.000 33.000 35.000 35.000 34.000 34.000 21.6 36.000 31.000 33.000 31.000 30.000 37.000 35.000 33.000 43.2 37.000 34.000 34.000 31.000 31.000 31.000 37.000 34.000 86.4 34.000 34.000 36.000 34.000 35.000 33.000 34.000 35.000 100 38.000 37.000 37.000 34.000 32.000 38.000 35.000 39.000 Intake 33.000 29.000 33.000 33.000 33.000 36.000 33.000 32.000 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Control-1 31.200 0.9968 31.200 28.000 34.000 6.193 10 Control-2 31.300 1.0000 31.300 29.000 34.000 5.645 10 10.8 33.700 1.0767 33.700 31.000 36.000 4.435 10 -2.828 21.6 32.800 1.0479 32.800 30.000 37.000 7.578 10 -1.810 43.2 33.800 1.0799 33.800 31.000 37.000 6.660 10 -2.941 86.4 34.800 1.1118 34.800 33.000 37.000 3.532 10 -4.072 100 36.500 1.1661 36.500 32.000 39.000 5.954 10 -5.995 Intake 33.200 1.0607 33.200 29.000 36.000 5.991 10 Tests Statistic Kolmogorov D Test indicates non-normal distribution (p <= 0.05) 0.98421 Bartlett's Test indicates equal variances (p = 0.35) 5.58769 The control means are not significant!}: different = 0.12078 Test {1-tail, NOEC LOEC ChV TU MSDu Dunnett's Test 100 >100 1 2.02145 0.06479 Treatments vs Control-1 lVA/ SQN 101 DMR-Discharge Monitoring Report CD-Ceriodaphnia dubia 9 30.000 29.000 31.000 32.000 34.000 36.000 37.000 36.000 1-Tailed Critical 2.287 2.287 2.287 2.287 2.287 Critical 0.895 15.0863 2.10092 MSB 32.12 10 32.000 31.000 32.000 30.000 35.000 37.000 38.000 34.000 MSD 2.021 2.021 2.021 2.021 2.021 Skew Kurt -0.0927 -0.4597 MSE F-Prob df 3.90741 7.9E-06 5,54 File: sqn101_081412data.xlsx Entered by: J. Sumner Reviewed by:+ Start Date: 8/14/2012 TV A I Sequoyah Nuclear Plant, Intake Non-treated August 14-21, 2012 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Test ID: CdFRCR Sample ID: TVA I SQN 101 -Intake End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Cone-% 1 2 3 4 5 6 Control-1 30.000 33.000 29.000 33.000 31.000 28.000 Control-2 30.000 34.000 34.000 31.000 32.000 29.000 10.8 36.000 34.000 33.000 33.000 35.000 35.000 21.6 36.000 31.000 33.000 31.000 30.000 37.000 43.2 37.000 34.000 34.000 31.000 31.000 31.000 86.4 34.000 34.000 36.000 34.000 35.000 33.000 100 38.000 37.000 37.000 34.000 32.000 38.000 Intake 33.000 29.000 33.000 33.000 33.000 36.000 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max Control-1 31.200 0.9968 31.200 28.000 34.000 Control-2 31.300 1.0000 31.300 29.000 34.000 10.8 33.700 1.0767 33.700 31.000 36.000 21.6 32.800 1.0479 32.800 30.000 37.000 43.2 33.800 1.0799 33.800 31.000 37.000 86.4 34.800 1.1118 34.800 33.000 37.000 100 36.500 1.1661 36.500 32.000 39.000 Intake 33.200 1.0607 33.200 29.000 36.000 Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.05) F-Test indicates equal variances (p = 0.73) The control means are not significantly different (p = 0.91) Hypothesis Test (1-tail, 0.05) Homoscedastic t Test indicates no significant differences Treatments vs Control-2 CV% 6.193 5.645 4.435 7.578 6.660 3.532 5.954 5.991 7 8 9 10 34.000 32.000 30.000 32.000 32.000 31.000 29.000 31.000 34.000 34.000 31.000 32.000 35.000 33.000 32.000 30.000 37.000 34.000 34.000 35.000 34.000 35.000 36.000 37.000 35.000 39.000 37.000 38.000 33.000 32.000 36.000 34.000 1-Tailed N t-Stat Critical MSD 10 10 10 10 10 10 10 1 0 -2.258 1. 734 1.459 Statistic Critical 0.91798 0.905 1.2669 6.54109 0.12078 2.10092 MSDu MSDp MSB MSE 1.45886 0.04661 18.05 3.53889 Skew Kurt -0.1715 0.33113 F-Prob df 0.03657 1, 18 Dose-Response Plot 45 40 35 0 30 +I g 25 "0 e 20 Q. Q) 0:: 15 10 5 0 ...... I :g 1:: 8 <X) <C! 0 :g c;:j ...... 1:: 8 C'l ("I) co 'V <X) 1-tail, 0.05 level of significance File: sqnl 01_081412data.xlsx Entered by: J. Swnner Reviewed by:+- I I I Species: Ceriodaphnia dubia Client: TV A I Seguoyah Nuclear Plant. OutfalllOl CONTROL 10.8% 21.6% 43.2% 86.4% 100% 100% Intake Page 6 of7 Date: _-3o&...::i:::.-*--='......!'-l._* .:_l ,_._.:::::._ __ *Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench

sheet specific for each analysis and transcribed to this bench sheet by: ' SOP AT11-Exhibit AT11.2, revision 07-01-12 tration CONTROL 10.8% 21.6% 43.2% 86.4% 100%. ). 100% Intake !ET *
EnvlronmentalTestingSolutlons.lnc.

Page 7 of7 Species: Client: Date: OC* Parameter

Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: SOP A Til-Exhibit AT11.2, revision 07-01-12 Control 10.8% 21.6% 43.2% 86.4% 100% 100% Intake TV A I Sequoyah Nuclear Plant, Outfall101

-Non-treated August 14-21, 2012 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Project number: ______ 82_0_7 File: sqn101_081412chem.xls Entered by: J. Sumner Reviewed by: Q{

11-, -I I I! I li "' r I I I I Page 1 of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Seguoyah Nuclear Plant NPDES #: TN0020168 Project #: --=':......;1.=-tr\=......a... ____ _ Dilution preparation information: Dilution prep(%) 10.8 21.6 Effluent volume (mL) 270 540 Diluent volume (rnL) 2230 1960 43.2 1080 1420 Total volume (mL) 2500 2500 2500 Test organism information: 86.4 2160 340 2500 Organism age: ""'lt)

1.1.. Uftal Jt C:.. Q l.b Date and times organisms Ol

\1... \\o()() were born between: 100 2500 0 2500 Organism source: ATb"" ot*a?t*tl.. Transfer bowl information: pH= i.\oO s.u. Temperature = 1..S.\ oc Average transfer volume: "' County: Rhea Outfall: 101 Comments: Each concentration was UV -treated for 2 minutes to remove pathogenic Interferences. Test information: Randomizing template: Incubator number and ?lf> shelf location: Artemia CHM number: t.l'Kt.S1-Drying information for weight determination: Date I Time in oven: ol*"L\* \1.. Initial oven temperature: \o() *t.-Date I Time out of oven: Ol*"tLa \"'J. Final oven temperature: Total drying time: Daily feeding and renewal information: Day 0 2 3 4 5 6 7 lndependen0 Review by Kelley E. Keenan ---Date

Morning feeding Afternoon feeding Test initiation,

renewal, or termination Sample numbers used MHSW batch used SOP AT20-Exhibit AT20.3, revision 07-01-12 I I I I I I I I I Page 2 of6 Species:

Pimephales promelas Client: TV A I Sequoyah Nuclear Plant, OutfalllOl, UV -treated Survival and Growth Data Day L:U.NT.KOL 10.8% 21.6% A B c D E F G H I J 0 10 10 /{) It> 10 /D /D ID /() /C 1 ro 10 10 10 10 10 10 10 /0 /0 2 10 10 10 tO 10 /0 10 /Q 10 /0 3 {f;J I() /Q l.O 10 10 /0 IO IO 10 4 /0 ID /0 /0 10 /0 /0 /0 IO /Q 5 '.0 10 /Q' /() /Q 10 10 IO /0 ID 6 --10 10 to tO 10 IO /0 10 IO ro 7 /0 1 ll) ((J I /0 I(J 10 ID (() tO A= Pan weight (me Tray color code:: ;E: 6lu, t4.4b 13.33 t4.te,z 14.z.c. lt.B4 Analyst: M IZ.SS tS.*1 t4.!S 13.1'\ Date: nB*QO*tt. B =Pan + Larvae weight (mg) Analyst: 17.'1 '8 l'f.Z S -t.l-3' [1.0. 0' 2.0. '11 z.as7 \4.1'8 ZD:!£1 I'S-"13 Date: C =Larvae weight (mg) = B-A Hand calculated. S.\0 "\.lt s.&1 IS.'\"\ S.'Y\ $ .. Analyst: . Weight per initial number of larvae (mg) r = C I Initial number of larvae " ,...., "':>... .,_,., " Hand calculated. "' \0 " \D t.; c, &"i o* Analyst: r::l o* 1(). C>. o* o* o* b. Average weight per Percent reduction

0. \:a\'-\ -, .... 4\ 7.

initial number of from control larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sin =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: K L /() IO /0 10 10 10 10 10 10 10 /0 IQ '0 10 10 10 14.0G, 13.tt 1'1.53 1$-13 s."\1 &"L \,'V t.; 1.;; f:). o* . SOP AT20-Exhibit AT20.3, revision 07-01-12 I I I I I TS Page 3 of6 Species: Pimephales promelas Client: TVA I Sequoyah Nuclear Plant, OutfalllOl, UV-treated Date: Survival and Growth Data Day 43.2°/o 86.4o/o 100% ; M N 0 p Q R s T u v 0 ro ro to lC) ID lQ IO /C) 10 tO 1 10 10 10 10 10 tO /0 lD /{) 10 2 (0 (0 /0 I() 10 tO /C 10 10 I 10 3 IO lQ 10 10 to /0 10 10 10 10 4 JD 10 10 10 ID 10 I() /Q 10 10 5 10 10 IO 10 IO IO 10 q*tA /0 IO 6 /0 /0 IC /0 /D IO 10 '1 JC /Q 7 It) ID lP\ I (() fO 10 /0 /() '1 ID A= Pan weight (mg) Tray color code:: t4.oB 13.0 13.2.1 *3.Gf\ t.f.sz. 14.33 ,3,41 Analyst: Date: a8*aL.*rz. B =Pan + Larvae weight (mg) Analyst:

s

'Ul 7..t>.oo

\8.'3 ICJ.o5 tS.3' '2J.1G 14."17 ,q .. 5C:J tq,"a5 ltl'g Date: C = Larvae weight (mg) = B-A Hand calculated. s .4\1. S.l'- s.tt s.ot\ '$.1."'\ "*" , ... Analyst: Weight per initial number of larvae (mg) ' = C I Initial number of larvae t' if' ,t ,_,o Hand calculated. t;; ,_, " ,.., Analyst: o' o* o* o* o* (:;) f;;;). '? o* o* Average weight per Percent reduction .() 1, o .soC\ initial number of from control (%) c.t *. &T. larvae(mJd Comment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: w X 10 10 IQ 'O 10 /D 10 10 10 10 IO IO 10 /0 lb 1(3*' 13.z.4. IZ.tC. 17.70 t.\.41\'-l ,..,\/} ,., o* o* o. \ SOP AT20-Exhibit AT20.3, revision 07-01-12 I I I I I TS Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant, OutfalllOl, UV-treated Day 0 1 2 3 4 5 6 7 A= Pan weight (mg} Tray color code:: Slw.. Analyst: __ Date: oA.ot. *\1.. B =Pan + Larvae weight (mg) Analyst:

n..& Date: 09*2.7.&2. C = Larvae weight (mg) = B-A Hand calculated.

A Analyst: -Weight per initial number oflarvae (mg) = C I Initial number of larvae Hand calculated. Analyst: __ Average weight per Percent reduction initial number of from control(%) larvae 10 10 /0 10 ID (() /0 10 10 10 /Q IO 10 10 tO /0 IC /() 10 IS.2.2, 14.lot 1."13 to.3'f 9'f tcr. z.s Ct. "1..\ 5 * "'*' Date: 0 &

a'=\* I,__ Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk = sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.

Comments: Page 4 of6 SOP AT20 -Exhibit A T20.3, revision 07-01-12

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Environmental Testing Solutions, Inc. Concentration(%)

Replicate A Control B c D E 10.8% F G H I 21.6% J K L M 43.2% N 0 p Q 86.4% R s T u 100% v w X y 100% Intake z AA BB Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnett's MSD value: PMSD: Initial number of larvae 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 0.0685 12.8 0.0826 15.5 Final number of larvae 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 9 10 10 10 10 10 10 10 10 A-Pan weight (mg) 12.88 14.40 15.49 14.63 13.33 14.62 14.26 12.84 14.35 13.19 14.06 13.11 14.08 13.47 13.53 13.17 13.27 13.69 13.96 14.82 14.33 12.73 13.24 12.76 15.22 14.61 14.37 14.39 MSD= PMSD= TV A I Sequoyah Nuclear Plant, Outfall101 UV-treated August 14-21, 2012 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Not for Compliance Assessment, Internal Laboratory QC B-Pan+ Larvae Larvae weight (mg) Weight I Surviving weight(mg) =A-B number oflarvae (mg) 17.98 5.10 0.510 19.28 4.88 0.488 21.36 5.87 0.587 20.16 5.53 0.553 20.06 6.73 0.673 20.21 5.59 0.559 20.57 6.31 0.631 18.78 5.94 0.594 20.34 5.99 0.599 18.43 5.24 0.524 19.53 5.47 0.547 18.93 5.82 0.582 20.00 5.92 0.592 18.63 5.16 0.516 19.01 5.48 0.548 19.05 5.88 0.588 18.36 5.09 0.509 18.96 5.27 0.527 19.47 5.51 0.551 19.30 4.48 0.498 19.83 5.50 0.550 18.38 5.65 0.565 18.50 5.26 0.526 17.70 4.94 0.494 21.43 6.21 0.621 20.34 5.73 0.573 20.84 6.47 0.647 19.21 4.82 0.482 Minimum Significant Difference Percent Minimum Significant Difference Mean weight I Coefficient of variation Surviving number of (Menweightpersuniving larvae (mg) numberorlarvae)(%) 0.535 8.3 0.614 8.0 0.563 6.0 0.561 6.4 0.521 4.5 0.534 5.8 0.581 12.5 Weight I Initial number of larvae (mg) 0.510 0.488 0.587 0.553 0.673 0.559 0.631 0.594 0.599 0.524 0.547 0.582 0.592 0.516 0.548 0.588 0.509 0.527 0.551 0.448 0.550 0.565 0.526 0.494 0.621 0.573 0.647 0.482 Project number: 8207 Mean survival Mean weight I Initial Coefficient of Percent reduction from (%) number oflarvae variation (Mean weight control(%) (mg) perinitialnumberoflanrae) (%) 100.0 0.535 8.3 Not applicable 100.0 0.614 8.0 -14.9 100.0 0.563 6.0 -5.3 100.0 0.561 6.4 -5.0 97.5 0.509 8.6 4.8 100.0 0.534 5.8 0.1 100.0 0.581 12.5 -8.7 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEP A (1Oth percentile) = 12%. Upper PMSD bound determined by USEP A (90th percentile) = 30%. Lower and upper PMSD bounds were determined from the I Oth and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEP A, 2001 a; USEP A, 2001 b). USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effiuent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: sqn101_081412data-uv.xlsx Entered by: J. Sumner Reviewed by: Jf--

* * *
6 Environmental Testing Solutions, Inc. Start Date: 8/14/2012 End Date: 8/21/2012 Sample Date: August 2012 Comments:

UV-treated Cone-% 1 D-Control 0.5100 10.8 0.6730 21.6 0.5990 43.2 0.5920 86.4 0.5090 100 0.5500 Intake 0.6210 Cone-% Mean D-Control 0.5345 10.8 0.6143 21.6 0.5630 43.2 0.5610 86.4 0.5088 100 0.5338 Intake 0.5808 Auxilia!l Tests 2 0.4880 0.5590 0.5240 0.5160 0.5270 0.5650 0.5730 N-Mean 1.0000 1.1492 1.0533 1.0496 0.9518 0.9986 1.0865 TVA I Sequoyah Nuclear Plant, Outfall101 UV-treated August 14-21, 2012 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Test ID: PpFRCR Sample ID: TVA I SQN 101 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas 3 4 0.5870 0.5530 0.6310 0.5940 0.5470 0.5820 0.5480 0.5880 0.5510 0.4480 0.5260 0.4940 0.6470 0.4820 Transform: Untransformed 1-Tailed Isotonic Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean 0.5345 0.4880 0.5870 8.269 4 0.5744 1.0000 0.6143 0.5590 0.6730 7.973 4 -2.807 2.410 0.0685 0.5744 1.0000 0.5630 0.5240 0.5990 6.009 4 -1.003 2.410 0.0685 '0.5630 0.9802 0.5610 0.5160 0.5920 6.414 4 -0.933 2.410 0.0685 0.5610 0.9767 0.5088 0.4480 0.5510 8.649 4 0.906 2.410 0.0685 0.5213 0.9075 0.5338 0.4940 0.5650 5.806 4 0.026 2.410 0.0685 0.5213 0.9075 0.5808 0.4820 0.6470 12.504 4 Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94963 0.884 -0.1675 -1.1562 Bartlett's Test indicates egual variances {E = 0.97l 0.84084 15.0863 Test {1-tail, 0.05l Dunnett's Test Treatments vs D-Control Point IC05 IC10 IC15 IC20 IC25 IC40 IC50 % 59.875 >100 >100 >100 >100 >100 >100 0.8 0.7 0.6 J 0.5 " 0.4 c 0.3 ..... 0.2 0.1 0 :g 1: 0 0 0 SD ()() 0 NOEC LOEC ChV TU MSDu MSB MSE 100 >100 0.06848 0.12811 0.00527 0.00161 Linear Interpolation (200 Resamples) 95% Skew 1.0 0.9 0.8 0.7 0.6 Q) 0.5 Ill c 0.4 0 m-0.3 0:: 0.2 0.1 0.0 -0.1 -0.2 -0.3 Dose-Response Plot '<I: CD co 0 0 0 *,' 50 Dose% 1-tail, 0.05 level of significance 100 F-Prob df 0.02854 5, 18 150 File: sqn101_081412data-uv.xlsx Entered by: J. Reviewed by: ----w Start Date: 8/14/2012 End Date: 8/21/2012 Sample Date: August 2012 Comments: UV-treated Cone-% 1 D-Control 0.5100 10.8 0.6730 21.6 0.5990 43.2 0.5920 86.4 0.5090 100 0.5500 Intake 0.6210 Cone-% Mean D-Control 0.5345 10.8 0.6143 21.6 0.5630 43.2 0.5610 86.4 0.5088 100 0.5338 Intake 0.5808 Auxiliary Tests 2 0.4880 0.5590 0.5240 0.5160 0.5270 0.5650 0.5730 N-Mean 1.0000 1.1492 1.0533 1.0496 0.9518 0.9986 1.0865 TV A I Sequoyah Nuclear Plant, Intake UV-treated August 14-21, 2012 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Test ID: PpFRCR Sample ID: TVA I SQN 101 -Intake Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas 3 0.5870 0.6310 0.5470 0.5480 0.5510 0.5260 0.6470 Mean 0.5345 0.6143 0.5630 0.5610 0.5088 0.5338 0.5808 4 0.5530 0.5940 0.5820 0.5880 0.4480 0.4940 0.4820 Transform: Untransformed Min Max CV% 0.4880 0.5870 8.269 0.5590 0.6730 7.973 0.5240 0.5990 6.009 0.5160 0.5920 6.414 0.4480 0.5510 8.649 0.4940 0.5650 5.806 0.4820 0.6470 12.504 1-Tailed N t-Stat Critical MSD 4 4 4 4 4 4 4 -1.088 1.943 0.0826 Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) F-Test indicates egual variances (p = 0.44) 0.95559 2.69933 0.749 47.4683 -0.6317 -0.2636 Hypothesis Test (1-tail, 0.05) Homoscedastic t Test indicates no significant differences Treatments vs D-Control 0.8 0.7 0.6 t 0.5 e C) 0.4 c ...... 0.3 0.2 0.1 0 :g <X) CC! c::i N c: .... 0 (.) 0 MSDu MSDp MSB 0.0826 0.15453 0.00428 Dose-Response Plot 1-tail, 0.05 level of significance MSE F-Prob df 0.00361 0.31833 1, 6 File: sqn 1 0 1_ 081412data-uv.xlsx Entered by: J. Sumner Reviewed

v. !ETS

.. lnc. Species: Pimephales promelas Date: ___ Client: TVA I Seguoyah Nuclear Plant. OutfalllOl. UV-treated CONTROL UV-treated 10.8% 21.6% 43.2%" 86.4%. 100% 100% Intake *Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed b : ' . Total residual chlorine was performed on non-treated Outfall! 01 and Intake samples. Page 5 of6 tration Parameter CONTROL UV-treated 10.8% 21.6% 43.2% 86.4% 100% 100% Intake Page 6 of6 .t *Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet ,deftt-.:>,-"M,-** Total residual chlorine was performed on non-treated Outfall 101 and Intake samples. SOP AT20-Exhibit AT20.3, revision 07-01-12 Concentration Control 10.8% 21.6% 43.2% 86.4% 100% 100% Intake -

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TVA I Sequoyah Nuclear Plant, Outfall101-UV-treated August 14-21, 2012 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number: ______ 8_2_07_ File: sqn101_081412chem-UV.xls Entered by: J. Sumner Reviewed by:+

.. =.E*,***r.*.*

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6 Environmental Testing Solutions, Inc. Analyst I Date analyzed

==o'3=*=' 'i=*=,'t.======= Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Titrate samples to pH= 4.5 S.U. Page _7..:...:6=--- Page -+--_of 3 Time initiated I t5o3 Time completed

\ =' Ll='f=======

Titrant normality and multiplier determination: pH of Normality Normality (N) of H2S04 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na2C03 x 0.05)/E = (N x 50000)1100 ml sample water reference standard ml ml ml =0.25/E =Nx500 =4.5 s.u. number number (E) (acceptable range= 0.0180 -0.0220) SJ

Z:NSS leW&' 0.0 \'t.' \'t*\ o.o-z.o7

\0.3 1!1\k cotr. o.o-c>.o.o.o L b I d d a oratory contro stan ar, : Reference standard True value Sample Alkalinity (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO:YL) (acceptable range (mg CaCO:YL) (ml) ml ml ml =90 to 110%) XN.S:S 1 o3/ 100 100 l'l.. \ 1:\."7 q.c 10.3 "" '\'\.0% Alkalinity %RPD= volume Begin End Total Multiplier (mg CaCO:YL) {(S-D) /[(S+D)/2]} X 100 ml ml ml (acceptable range=+/- 10%) '1.-'*7 t.7 . ., ,,0 tO..l s 't .11 Duplicate (B) D "2."7.7 "!.'3 * ..., ,,0 '1. -S-" atnx spz e recovery: ... Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total (mg CaCO:YL) (mg CaCO:YL) (ml) ml ml ml -::r.:Ns.s 1037 *so lCb z'/.7 31$.5 ,o.s (().3 l IO Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCO:YL) MV=A-B (acceptable range (mg CaCO:YL) =75 to 125%) 62 Lf'S I amp4 e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml ' Multiplier (mg CaCO:YL) lQ:) 38.5 'i4.S ,,0 10.3 '1.. 00 .\1. *.VI,.-l o.o ,,() ,,0 '2. l'L-6.() \7.'3 \\.'3 \2.0 .. ,o*tt. r!l J 17.3 "t.C0.3 \\.0 \10 "' ,If Z.1.3 31.t. \O.'J \(() 6"0 L(S.? ,,s ('Z.) l"30 0 t. \"'2. .. ml-K. vv 1 '():;) c>.o '*G 'z a& .,'-\ ... ,'1... \ 2. b.O 1\.'\ S.'f O:t*l\G*\-z.._ .lJ 3 'II l\.'1 s . ..., ,, Reviewed by: Date reviewed: I oe, l q **"Z-SOP C6 -Exhibit C6.1. revision 06-01-11 -;:> .. -.* ,**.*.*.****,*.* .. r I .E.T . : .... ! *... *. 6 Environmental Testing Solutions, inc. Analyst I Date analyzed Alkalinity (SM 2320 B) Matrix: RL = 1.0 mg CaC03/L Titrate samples to pH= 4.5 S.U. Page If Page -z... of-'3=---- Time initiated p I completed Titrant normality and multiplier determination: pH of Normality Normality (N) of H2S04 pH Factor or Multiplier J)eiOmzeu ...... ,.h,.,.lr Beein End Total = (5 ml Na2C03 x 0.05)/E = (N x 50000)/100 ml sample water reference standard ml m* --ml-r-:--_ = 0.25/E =Nx500 4.5S.U. number number (E)

..,. n n1 flln -0.0220) L b a d I a oratory contro stan ar : Reference standard True value Sample Alkalinity (MV) % RS=MV /TVx 100 number (TV) volume Begin End Total Multiplier (mgCaCOYL)

(acceptable range (mg CaC031L) (ml} ml ml ml =90 to 110%) 1031 100 100 17.9 %.1. '3 q.S" \a.3 q9 Alkalinity %RPD= Begin End Total Multiplier (mg CaCOliL) {(S-D) /[(S+D)/2]} X 100 ml ml ml (acceptable range = +/- 10%) '2. ..,, 3 Jo,, s 72. '3.5 Duplicate (B) D 36-i '3'4.3 7Z "k atrrx spz e recovery:

- -Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaC031L)

(m2 CaC031L) (ml) ml ml ml s 1037 \00 '5D 30.8 '5.l l7c Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (mgCaCOYL) MV=A-B (acceptable range (mgCaCOYL) =75 to 125%) 7Z "" ' Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ill (ml) ml ml ml Multiplier (mg CaCOliL) \1.0 -lfJ .A. _c.Q.J *O a 2. fP 3'1.0 LIZ.. 'f 3.'-i (Z.) \0.3 7o . 17.01\1*01 -11 .3 "\2..'-l LfS.'t l.S 7'l. \1.01 hJi ' o.o 3.3 '3.3 'S \ "2.6t ,c;. ' \

2.. '3.3 '*' J.'3 *8 'to4 o1-.3 6.G \0,0 3.4 70 l 'Z.o..()t

!,. 0\ 1"""1 A &oauo 1 IO.O \'3. '-\ 3.Lt 70 2. o g \S* &0 ( 2 ll.Lf "*f 3.'1 70 a t.oB 11. oR-"' 3 16.9 '2.,.0.'l. 3,4 70 J z. o nt.. a 'Z.<>.Z. 2.1." l.Lf ,, 7o Reviewed by: Datereviewed: IOS*lq*l1.. SOP C6 -Exhibit C6.1. revision 06-01-11 =E***.** .. r** .. ****.s*.:'.*, . .. .. . * *

t Environmental Testing Solutions, Inc. Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Page --=-7l=-_,__

Page 3 of-=.3 __ Analyst I 3'"\J Date analyzed 1--o--s.....;;. ,-,-. 1-z----1 Titrate samples to pH= 4.5 S.U. Time initiated Time completed 0'1*\t*lt Titrant normality_ and multiplier determination: pH of Normality Normality (N) of H2S04 pH Factor or Multiplier _._ ...... check Begin End Total = (5 ml Na2C03 x 0.05)/E = (Nx 50000)/ 100 ml sample ...... water reference r--mL. ml ml =0.25/E =Nx500 s.u. number number r---f-...cEl (acceptable range = 0.0180 -0.0220) -u:.P C>T*l I'* tz. Lb I tl d. a oratory contro stan ar, : Reference standard True value Sample Alkalinity (MV) %RS =MV /TVx 100 number (TV) volume Begin End Total Multiplier (mg CaCO:VL) (acceptabie range (mg CaCO:VL) (ml) ml ml ml =90 to 110%) ""J:NSS \03'7 100 100 "Z.3.C' '33.\ C\.S to.3 Cf'S

. Alkalinity

%RPD= End Total Multiplier (mg CaCO:VL) {(S-D) /[(S+D)/2]} X 100 ml (acceptable range=+/- 10%) s ID td) D 3G.S 3'1.'1 70 Of*l8*tt. M. * 'k atrtx sp1 e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mgCaCOYL) (m_g_ CaCO:VL) (ml) ml ml ml :i:)\SS \03"7 \00 '3,,5 Ll"'.7 4.l. \0.3 l?o Sample alkalinity (B) Measured spike value (MV) % R = MV I sv X 100 (mg CaCO:VL) . MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%) 7o IOO 10o.o% amp4 e measurements: .. Sample volume Begin End Total Alkalinity Sample numper Sample ID (ml) ml ml ml Multiplier (m2 CaCOJI.Ll 3 *P Lf&f. 7 '3.4 C:Z..1 \0.3 76 ol tt..\ .. ' 100 d,, 1.0 0."1 J Lf .( \'!. () & l\o .o \ J 2.. I \.0 4.'-t 5.'4 35 11. otli.-o \ ..3 t '1.'"{ 7.' 3,5 3C .. ------,.....-----. ----------O"f*\'i*rl. Reviewed by: Date reviewed: I De*l q ,ft., SOP C6 -Exhibit C6.1. revision 06-01-11 Analyst I Date analyzed Titrant normality and multiplier determination: Titrant Normality check Begin End Total Hardness (SM 2340 C) RL = 1.0 mg CaC03/L Total Normality (N) of EDT A _,,,_,-:,r __ ,ic _:,:;.'-. pH Factor or reference standard ml ml ml =0.2/E = (Nx soooo)t50mlsafuple number number (acceptable range = 0.0180 -0.0220) (E) =Nxtooo _ INI:. ":J:NSS to:sJ o.o IO.'t \O."L 0.0\'l' \Cf.C -* L b l d d a oratory contro stan ar : Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaC03/L) (ml) ml ml ml =90 to 110%) :Z:J.l.SS l 0 4 i' 40 50 IQ.'Z.. 1'2. "Z 'Z..O ,q,, 'act Cf7.5% Sample Hardness %RPD= Sample Sample ID volume Begin End Total Multiplier (mg CaC03/L) {(S -D) /[(S+D)/2]} X 100 number ml ml ml 6) \'l.:Z. 4.5 \<t.6 s S) D ,,_ '1 1. \. t '-(.5 '815 atnx spz e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaC03/L) (ml) ml ml ml ::CNS.S \OLff 40 *7 z.3."Z ,.5 \Cf.C \'30 Sample hardness (B) Measured spike value (MV) % R=MV /SVx 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%) 42.. \oS.o% ampJ e measurements: Sample volume Begin End Total Hardness SaQJ.ple number SampleiD (ml) ml ml ml Multiplier (mg CaC03/L) TV=ND Blank 60 ND (should be = 0 mg CaC03/L) O.c> 0.0 o.o I cr.C (.)e,) 11 .. IY\W5W 2.3.'2-1..7.-, t.t. 5 I 'a <a' oe .. ,.,_rt... Jt '3'2.1.( '1.7 'f2 (>a' 1\A-32.4 33.$ I. l zz 04 .. \'Z.* 1; In t*S Lh.J ' .n.s 37.S' .... oa *a'-'\ .. ',_ ' 2.. 37.'i L(l.1 LI.J OJ*) 'o_*l 'l.t Jt 3 L(Z..I "f6.&.f Lf-3 '1'J 1\ 10\ \ 0,0 3.7 '3.1 -rz. l'I.Ot\S. lO l z. 3.?' '7.3 '3h' 7l rz. oa a.,. 08-3 7.!, tt.\ "'3.'ir 7£{ Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: Date reviewed I ,..... .,. ..... --.... (t Page 3'7 @ *@J Page 7 of 3 Analyst I Date analyzed

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Titrant normality and multiplier determination: , '!!!!_ant Normality check Begin End Total Normality (N) of EDT A pH Factor or Multiplier reference -* -*-. .. = 0.21E = (N x 50000)1 50 ml sample ., ...... ucuu 1111 .... number number (E) (acceptaun:: n OHIO-0.0220) Nx 1000 L b t t I t d d a ora ory con ro s an ar, : Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaC03/L) (ml) ml ml ml =90 to 110%) 40 50 II.\ (3.0 ,_, l<t.C '37 1'Z..S?; Hardness %RPD= Begin End Total Multiplier (mg CaC03/L) {(S-D) /[(S+D)/2]} X 100 ml ml ml \3.0 * s 72. Duplicate (B) D IC.7 72. atrlX spz e recovery: .. Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaC03/L) (ml) ml ml ml :tJJ5S Lfo 8J \C. "7 'l'Z..'t 5."1 t1.C \\0 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaC03/L) =75 to 125%) 71.. ' "{S.o% s l amp4 e measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC03/L) T\r Hl..,.nlr l'ILJ (should be= 0 mg CaC03/L) '-z.oi\S- \\ 1\/A 'Nl' 'L 6' l..'l..Lf 2&:Z '3.t l'f \7J>& \1 .. 0'\ J, .3 '1.,, 'Z. 1..'\,q '3:7 7Z-\ .OZ....o.i ' !. "() \ Tv A S&a..lao,uu ' 33., 3.7 7Z j 2.. '3'3., 37.'!1 '3.7 71. 3 37.3 Lfi.O 3.7 n I "'1. OJr\ * (J1.. TJ A ' lfl.o 4'f.C 3.-G 71 \ 1..0& l\ z.. 4"t., Lt1.3 3."7 -rz. \ '"2. 0' ,,_ d\ 3 o.o '3.1 '3.7 7'Z. 1t.l. 0\ 3:7 S.1 't.. \ Lfl Note: If >15ml oftitrant is used, sample must be diluted. Reviewed by: *I Date reviewed I re 'q I,_ SOP C7 -Exhibit C7 .1. revision 06-01-11

$ ;l':\1 '3> Environmental Testing Solutions, Inc. Page ({o Page 3 of 3 ___::;___

Total Hardness (SM 2340 C) RL = 1.0 mg CaCOiL Analyst ___ -t Date analyzed o <6'. t1* \ -t. Titrant normality and multiplier determination: , Titrant Normality check Begin End -" -" number number L b d d I a oratory contro stan ar : . Reference standard True value number (TV) (mg CaCOJIL) XNSS toL{ 'IS 40 number ..... ml Sample volume (ml) 50 Sample volume Sample hardness (B) (mg CaC03/L) Sam le measurements: Total ml (E) Begin ml 5".'8' Begin ml End ml 7.7 End ml Sample volume ml \""l. at --*0\ \ "l. ot ' &

o \ Note: If >15ml oftitrant is used, sample must be diluted.

Reviewed by: Time initiated 1-::-:---..... Time completed <:::::J\B Normality (N) of EDTA pH Factor or Multiplier 0.2/E (N x 50000)/ 50 ml sample (acceptable range nO??m Nx 1000 Total Multiplier ml ; v:; \ct.C Total Multiplier ml Multiplier Hardness (MV) (mg CaCOJIL) '31 Hardness (mg CaCOJIL) s D fl. % RS = MV I TV x 100 (acceptable range =90 to 110%) crz.59: %RPD= {(S -D) /[(S+D)/2]} X 100 Spike hardness (A) (mg CaC03/L) Begin End Total Hardness ml ml ml m CaCOJIL) ?'\D l'i. rz 7.7 \(.0 3.3 \ct., '-S lt.G \5.5 '"l.S J, Date reviewed I 06 t91l-SOP C7 -Exhibit C7 .1. revision 06-01-11 l I Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pH/Ion Meter Page ___._'f'-=--z.._ Page_,_ of_( _ Analyst I Date analyzed

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reagent: Acid reagent: 'IN<< 5\"1 Calibration: Lb I a oratory contro stan d d ar,: Reference standard True value (TV) number (mg/L) :!NSS '0'"' 0.50 Sample characteristics Sample ID Measured value (MV) (mg/L) o.Sl-41 %RS=MV /TVx 100 (acceptable range= 90 to 110%) loS.i% %RPD = {(S-D) /[(S+D)/2]} X 100 (acceptable range= :1:: 10%) Sample characteristics Note: All samples were analyzed in excess ofEPA recommended holding time (15 minutes) unless otherwise noted. L b t I d d a ora ory contro stan ar, : Reference standard True value (TV) Measured value (MV) % RS=MV /TVx 100 number (mg/L) (mg/L) (acceptable range= 90 to 110%) 'J:NSS lO'"i 0.50 O .. c.f71 et'f.z..'tc, Reviewed by I Date reviewed SOP C8 -Exhibit C8.1, revision 06-01-11 I * * * *

Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pH/Ion Meter Page cts Page _l_. of _j___ Analyst I Date analyzed

o=S=*=tC=*=fl..========

Iodide reagent: ____ -1 Acid reagent: l:N t St? Calibration: Lb I a oratory contro stan d d ar : Reference standard True value (TV) Measured value (MV) % RS=MV /TV X 100 number (mg/L) (mg/L) (acceptable range= 90 to 110%) :;J:.J\)SS IOC"f 0.50 0.532 \0'*""' Sample characteristics Duplicate D..Lo.oottS Sample ID Sample characteristics o.otttl LO.OfLil"f <.o.ool3lf '-O.oo ISl Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Lb I a oratory contro stan d d ar : Reference standard True value (TV) Measured value (MV) % RS=MV /TV X 100 n*umber (mg/L) (mg/L) (acceptable range= 90 to 110%) l GC...., 0.50 O.L(CS Reviewed by I X Date reviewed

===o=r-t:*

.. :,:\=,*=\=1-===: SOP C8 -Exhibit C8.1, revision 06-01-11

* * *
Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pHil on Meter Page '7 Page __L_ of_l _ Analyst reagent:

Date analyzed . Actd reagent:

rtJ rt 5,7 Calibration:

Lb I d d a oratory contro stan ar : Reference standard True value (TV) Measured value (MV) % RS=MV /TV X 100 number (mg!L) (mg/L) (acceptable range= 90 to 110%) J:l.)SS J06'f 0.50 0.520 Sample characteristics Duplicate SampleiD Sample characteristics LG.003ft: no Co Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. L b I d d a oratory contro stan a11 : Referen.ce standard True value (TV) Measured value (MV) %RS=MV /TVx 100 . number (mg!L) (mg/L) (acceptable range= 90 to 110%) :Z:J"l...g' l06Lf 0.50 o. 'iS"Cf C{l.'SY. Reviewed by Date reviewed OJ>i6-I 4 SOP C8 -Exhibit C8.1, revision 06-01-11

Sequoyah Nuclear Plant Biomonitoring August 14 - 21, 2012

Appendix D Reference Toxicant Test and Control Chart

-u ..... "Qa -in N u -> "' "C I "' Pimephales promelas Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc. Environmental Testing Solutions, Inc. 0.9 0.8 0.7 0.6 0.5 1.4 1.2 1.0 0.8 0.6 0.4 1.4 1.2 1.0 0.8 0.6 0.4 USEPA Control Limits(+/- 2 Standard Deviations)

USEPA Warning and Control Limits (75th and 90th Percentile CVs) ******************************************************************************************************** -**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**

Laboratory Warning and Control Limits (lOth and 25th Percentile CVs) ******************************************************************************************************** ....... -**-**-**-**-**-**-**-**-**-**-**

Test date

7-day IC25 = 25% inhibition concentration.

An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. ............... .............. Central Tendency (mean IC25) -**-**-Warning Limits (mean IC25 +/- sA.lO or sA.75)

Control Limits (mean IC25 +/- SA.25, SA.90, or 2 Standard Deviations)

Graphs generated from associated excel spreadsheet. Excel spreadsheet entered by: J. Sumner Reviewed Test number Test date 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 08-09-11 09-13-11 10-04-11 10-11-11 10-18-11 11-08-11 12-06-11 02-07-12 02-14-12 03-06-12 03-06-12 04-12-12 05-08-12 05-15-12 06-05-12 06-12-12 07-10-12 07-17-12 08-07-12 08-14-12 7-day IC25 (g/L KCI) 0.67 0.66 0.73 0.76 0.67 0.78 0.77 0.77 0.74 0.64 0.68 0.71 0.77 0.71 0.72 0.77 0.76 0.73 0.70 0.81 CT (g/L KCI) 0.71 0.71 0.71 0.71 0.71 0.71 0.71 0.71 0.71 0.71 0.71 0.71 0.72 0.71 0.71 0.71 0.71 0.72 0.72 0.73 s 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 -*

* ---

- Pimephales promelas Chronic Reference Toxicant Control Chart State and USEPA Control Limits CT-25 0.62 0.61 0.61 0.62 0.61 0.61 0.61 0.61 0.61 0.61 0.61 0.61 0.61 0.61 0.61 0.61 0.61 0.62 0.62 0.63 CT+2S 0.80 0.80 0.81 0.81 0.81 0.82 0.82 0.81 0.82 0.82 0.82 0.82 0.82 0.82 0.82 0.82 0.82 0.81 0.81 0.82 0.09 0.08 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 Laboratory Warning Limits CT-SA.lO CT + SA.lO 0.63 0.62 0.62 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.64 0.80 0.79 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.81 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Laboratory Control Limits CT-SA.25 CT + SA.2S 0.56 0.56 0.56 0.56 0.56 0.56 0.56 0.56 0.56 0.56 0.56 0.56 0.57 0.56 0.56 0.56 0.56 0.57 0.57 0.57 0.86 0.86 0.86 0.86 0.86 0.86 0.86 0.86 0.86 0.86 0.86 0.86 0.87 0.86 0.86 0.86 0.86 0.87 0.87 0.88 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.28 US EPA Warning Limits CT-SA.7s 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.45 0.45 0.98 0.98 0.98 0.99 0.98 0.98 0.98 0.98 0.99 0.98 0.98 0.98 0.99 0.99 0.98 0.98 0.98 0.99 0.99 1.00 Note: 7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. CT = Central tendency (mean IC25). S = Standard deviation of the IC25 values. Laboratory Control and Warning Limits SA.90 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.33 US EPA Control Limits 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.40 0.40 1.03 1.03 1.03 1.03 1.03 1.03 1.03 1.03 1.04 1.03 1.03 1.03 1.04 1.04 1.03 1.03 1.03 1.04 1.04 1.05 cv 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.07 0.08 0.08 0.07 0.07 0.07 0.07 0.07 0.06 Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. SA.1o = Standard deviation corresponding to the 1dh percentile CV. (SA.lo = 0.12) SA.25 = Standard deviation corresponding to the 25th percentile CV. (SA.2s = 0.21) US EPA Control and Warning Limits SA.7s = Standard deviation corresponding to the 75th percentile CV. ( SA.75 = 0.38) SA.90= Standard deviation corresponding to the 90th percentile CV. (SA.90 = 0.45) CV = Coefficient of variation ofthe IC25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. File: ppkclcr_081412.xlsx Entered by: J. Sumner Reviewed -CIJ .c ta i 0 ..!!! ... -; 0 :e ... .5 +" c ........ 0 bO u E --'#. -c .c 0 +"' 3: ta 0 ... '+-0 0 ... +"' +" c c 0 CIJ u *o ... e CIJ 0 u -* Pimephales promelas Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Organism Source: Aquatox, Inc. 1.00 ... ... ... ***************************************************************************************** 0.75 0.50 ******************************************************************************************* .. ** ... 0.25 USEPA Acceptance Criteria (> 0.25 mg per surviving larvae) 30 Kentucky Acceptance Limit(< 30.0%) 20 ... .... *************

10 0 30 USEPA Upper PMSD Bound (90th percentile< 30.0%} 20 .. ******************************************* .* ************************************************** 10 ************************************************************************************************** Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) Graphs generated from associated excel spreadsheet. Excel spreadsheet entered by: J. Sumner Reviewed by: Test number 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Note: Precision of Endpoint Measurements Pimephales promelas Chronic Reference Toxicant Data Test date Control Control Mean cr cv cr Survival Growth (%) (mg/larvae) for Control Growth (%) for Control (mg/larvae) Growth CV (%) 08-09-11 100 0.662 6.6 09-13-11 100 0.848 0.755 6.7 6.7 10-04-11 100 0.768 0.759 16.8 10.0 10-11-11 100 0.681 0.740 4.3 8.6 10-18-11 100 0.693 0.730 4.8 7.9 11-08-11 100 0.792 0.741 10.3 8.3 12-06-11 100 0.738 0.740 15.7 9.3 02-07-12 100 0.662 0.730 10.5 9.5 02-14-12 97.5 0.658 0.722 5.2 9.0 03-06-12 100 0.854 0.735 3.5 8.4 03-06-12 100 0.873 0.748 5.1 8.1 04-12-12 97.5 0.706 0.744 7.0 8.0 05-08-12 100 0.613 0.734 8.1 8.1 05-15-12 100 0.697 0.732 5.9 7.9 06-05-12 97.5 0.596 0.723 3.8 7.6 06-12-12 100 0.723 0.723 6.0 7.5 07-10-12 100 0.641 0.718 5.2 7.4 07-17-12 100 0.638 0.713 10.9 7.6 08-07-12 97.5 0.660 0.711 6.7 7.5 08-14-12 100 0.533 0.702 5.4 7.4 CV = Coefficient of variation for control growth. Lower CV bound determined by USEPA (lOth percentile)= 3.5%. Upper CV bound determined by USEPA (90th percentile)= 20% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference MSD PMSD cr (%) for PMSD (%) 0.07 10.6 0.12 13.6 12.1 0.13 16.9 13.7 0.07 9.9 12.8 0.07 10.0 12.2 0.12 15.6 12.8 0.12 15.9 13.2 0.09 13.4 13.2 0.07 10.1 12.9 0.09 10.8 12.7 0.10 11.4 12.6 0.06 8.7 12.2 0.08 13.1 12.3 0.07 9.5 12.1 0.06 9.4 11.9 0.11 15.6 12.2 0.10 14.9 12.3 0.07 11.4 12.3 0.06 8.7 12.1 0.07 13.1 12.1 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA {lOth percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile) = 30%. cr = Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. US EPA. 200la, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: ppkclcr_081412.xlsx Entered by: J. Sumner Reviewed by:-+ , ____ :'_:-Page I of5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Dilution preparation information: KCl Stock INSS number: ,02'\ Stock preparation: 50 gKCIIL: Dissolve 50 g KCl in 1-L Milli-Q water. Dilution prep (mg/L) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Organism age: "ll '-\1. o..__b Date and times organisms 04* \'1>>* ,..,_ \'oCO were born between: Organism source: ()c. \'J. *l"'L Transfer vessel pH= , .. \,C s.u. information: Temperature = ,_Soc\ oc Average transfer v9lume: .. Daily fe_eding and renewal information: Day Date Morning feeding Afternoon feeding 0 2 3 4 PpKClCR Test Number: ___2..5S Comments: Test information: Randomizing template: Incubator number and .3b shelf location: Artemia CHM number: t..l\ tot t. Drying information for weight determination: Date I Time in oven: Initial oven temperature: Date I Time out of oven: Final oven temperature: Total drying time: 6& .. "l.\* \'L Co.b *t 6&*1.1. .. \'l.. \1.6tl 4oa *c.. "\).\.** -""' MHSW batch used

11.-.
11... SOP AT21-Exhibit AT21.1, revision 06-01-11

... I* n I I II;. I . . 1;,_ -I I I iET 6 Environmental Testing Solutions, Inc. Page 2 of5

Species:

Pimephales promelas PpKClCR Test Number: Survival and Growth Data Day Control 450 600 mg KCI/L ; .A B c D E F G H I -! K L 0 ' /0 /0 /Q (() 10 IQ /Q I() 10 /0 10 IO 1 /0 10 10 /0 10 10 IO 10 IO lO /0 10 2 10 10 10 10 /C 10 10 10 10 10 tO 10 3 lD tO {0 to IO lC IC 10 lO IC 10 (0 4 /0 I'D lO 10 10 IO 10 10 <i.l 10 10 s 10 /0 10 10 10 10 10 IC 'i 4 10 IO 6 /0 to (0 10 lO JC /0 10* '\ t\ (Q 10 7 10 'O /() /Q 10 '0 10 10 '1 '1 IQ 10 A= Pan weight (mg) Tray color code:: ... 14.gt.t \4.t3 13.88 IJ.43 13.81 t4.tn l3.bL rg.4z Analyst: Date: 08*03*U. / B = Pan + Larvae weight (mg) / Analyst: .:1\.B tg,53' Z."r l.fo I'I.'Z."' 11.,6 -\1.l, 'Z.O.ff Zl.Sc:> lB. So 'Z.0.3'Z. l't.'*IO l't.6Z. Date: 08*2:7.1'2. C = Larvae weight (mg) = B -A c,.'-'1 s.""' s.O"'\ s.s\ 'S.\\ s .. 1e S.1\o (...'1'1 t.f,C.'\ S.11 ,1.0 Hand calculated. /'V--Analyst: Weight per initial number of larvae (mg) . = C I Initial number of larvae q .,.,., ,¢-"" "'0 "\IJ Hand calculated. j\], ""' " " '0 '0 <<:; <<-;, '0 Analyst: .... o* o* b" o* \'). b

o* o* ()" (). t)" { Average weight per initial Percent number oflarvae (mg) reduction

o. t,01 -

-eo{. c; 7. from control (%) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk = sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: SOP AT21 -Exhibit A T21.1, revision 06-01-11 I

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I * * **

Species:

Pimephales promelas Day 750 KCIIL ; M N 0 p 0 JD /0 IO IO 1 /0 tO tO /0 2 /0 /0 "'" 10 3 (() 10 4 ID q '\ 5 /0 " q "' 6 q .a_ 10 7 " 'i '\ A= Pan weight (mg) Tray color code:: t3.\t 14.sc.. Analyst: '3.lB lZ.gt, Date: B =Pan + Larvae weight (mg) Analyst: .,-ur* 11."i4f 1'1. 'Z."' 1 s. '31 17.49' Date: ()1f*"Z. ..,,,z. C = Larvae weight (mg) = B-A Hand calculated. .... , 'l.c.a s.t"l. Analyst:

- .-......---'

Weight per initial number of larvae (mg) = C I Initial number of larvae '" !\ Hand calculated. :$!* ... (,) Analyst: --o* o* (). tl. Average weight per initial Percent number oflarvae (mg) reduction 0 ."118' JQ. ':,?. from control (%) Page 3 of5 PpKCICR Test Number: Survival and Growth Data 900 mg I(CIIL 1050 m! KCIIL Q R s T u v w X /0 IO IO 10 /Q IO 10 10 1IA **M {A 'ItA . 1-'. --\'-' .. .,. .B 1 '-3 g 1 1 (a '-\ *q I *o\ ., \o '-( lp 1 1 5 "2-'2... 3 , , s If\ 2 tl\, *fl \ "2-.,_ lo"c.\ ., s \ '-\ *it \ s'"' (o , \ "2..u c.J'"' o\( f4.S'\ .IZ.IB 14.11 13.t8 13.Z.-6 17.,, 17.ZS \G.'Z..l 17.30 \ 3."3, t"'.76 .. "(."<:6 a.s& ..... ** §l J' c 0 ' t;;l o* o* o* . 3l\.t'1. o.os 1. 16* 2 7. Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk = sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: SOP A T21 -Exhibit AT21.1, revision 06-01-11 Concentration (mc/L KO) Repllcete A Control B c D E 450 F G H I 600 J K L M 750 N 0 p Q 900 R s T u 1050 v w X Dunnett's MSD value: PMSD: lnltlal number of larvae 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 0.0699 13.1 Rnal number of larvae 10 10 10 10 10 10 10 10 9 9 10 10 8 9 9 9 5 6 7 5 1 2 0 0 A= Pan wellht (mg) 13.63 13.46 14.89 14.13 13.88 13.43 14.54 15.02 13.81 14.69 13.62 13.42 13.12 14.56 13.78 12.86 14.59 13.72 12.18 14.11 12.85 13.18 0.00 0.00 MSD= PMSD= Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses B=Pan+larvae Larvae weight (mg) = Weight I Surviving Mean weight/ Surviving Coafllclent of variation Weight I lnltlal number of welght(mg) A*B number of larvae (mg) number of larvae (m1) (Men wetcht per suMvlrc larvae(mg) numberoflo,.vaeJ(") 19.27 5.64 0.564 0.564 18.53 5.07 0.507 0.533 5.4 0.507 20.40 5.51 0.551 0.551 19.24 5.11 0.511 0.511 19.66 5.78 0.578 0.578 19.19 5.76 0.576 0.607 5.9 0.576 20.81 6.27 0.627 0.627 21.50 6.48 0.648 0.648 18.50 4.69 0.521 0.469 20.32 5.63 0.626 0.586 8.2 0.563 19.40 5.78 0.578 0.578 19.62 6.20 0.620 0.620 17.89 4.77 0.596 0.477 19.24 4.68 0.520 0.547 7.9 0.468 18.31 4.53 0.503 0.453 17.98 5.12 0.569 0.512 17.69 3.10 0.620 0.310 17.28 3.56 0.593 0.607 4.4 0356 16.23 4.05 0.579 0.405 17.30 3.19 0.638 0.319 13.36 0.51 0.510 0.051 14.76 1.58 0.790 0.650 30.5 0.158 0.00 0.00 0.000 0.000 0.00 0.00 0.000 0.000 Minimum Significant Difference Percent Minimum Significant Difference Test number: ____________ 25_5 Test dates: _______ A_u:.gu_s_t _14-_2_1..:.,_20_1_2 Mean survival (") Mean weight/ Jnltlal Coefficient of variation Percent reduction from number of larvae I" I control l"l (mg) 100.0 0.533 5.4 Not applicable 100.0 0.607 5.9 -13.9 95.0 0.558 11.4 -4.5 87.5 0.478 5.2 10.5 57.5 0.348 12.4 34.8 7.5 0.052 142.6 90.2 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10th percentile)

12%. Upper PMSD bound determined by USEPA (90th percentile)

30%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (US EPA, 200la; USEPA, 200lb). USEPA. 200la, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: ppkclcr_081412.xlsx Entered by: J. Sumner Reviewed by:-# Start Date: End Date: Sample Date: Comments: Conc-mg/L D-Control 450 600 750 900 1050 Conc-mg/L D-Control 450 600 *750 *900 *1050 8/14/2012

8/21/2012 1.0000 1.0000 0.9000 0.8000 0.5000 0.1000 Mean 1.0000 1.0000 0.9500 0.8750 0.5750 0.0750 Auxiliary Tests 2 1.0000 1.0000 0.9000 0.9000 0.6000 0.2000 N-Mean 1.0000 1.0000 0.9500 0.8750 0.5750 0.0750 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Test ID: PpKCICR Sample ID: REF-Ref Toxicant Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas 3 4 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 0.9000 0.9000 0.7000 0.5000 0.0000 0.0000 Transform: Arcsin Sguare Root Rank 1-Tailed Number Mean Min Max CV% N Sum Critical Resp 1.4120 1.4120 1.4120 0.000 4 0 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 1.3305 1.2490 1.4120 7.072 4 14.00 10.00 2 1.2136 1.1071 1.2490 5.846 4 10.00 10.00 5 0.8620 0.7854 0.9912 11.405 4 10.00 10.00 17 0.2757 0.1588 0.4636 53.294 4 10.00 10.00 37 Statistic Critical Skew Shapiro-Wilk's Test indicates normal distribution (p > 0.01) Eguality of variance cannot be confirmed 0.93302 0.884 0.39148 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 600 750 670.82 Treatments vs D-Control Trim Level EC50 95%CL 0.0% 5.0% 10.0% 902.19 867.55 938.21 20.0% 909.49 869.16 951.70 Auto-7.5% 898.26 863.27 934.67 0.9 0.8 I'CI 0.7 > *E o.6 :I (/) 0.5 Q 0.4 .... 0.3 0.2 0.1 0 :g 0 I() c: ..,. 0 () 0 0 0 co Trimmed Spearman-Karber 1.0 -.-----------------. 0.9 0.8 0.7 8! 0.6 c 8.. 0.5 0.4 0.3 0.2 0.1 J 0.0 +--r-r"TTTmr---.-rrrrmr--r'"T'4fnmr-"1""T"T-rrml 1 10 100 1000 10000 Dose mg/L Dose-Response Plot 0 0 0 I() 0 I() t'" sn 0 r Total Number 40 40 40 40 40 40 Kurt 0.28113 File: ppkclcr_081412.xlsx Entered by: J. Sumner Reviewed Start Date: 8/14/2012 End Date: 8/21/2012 Sample Date: Comments: Conc-mg/L D-Control 450 600 750 900 1050 0.5640 0.5780 0.4690 0.4770 0.3100 0.0510 2 0.5070 0.5760 0.5630 0.4680 0.3560 0.1580 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Test ID: PpKCICR Sample ID: REF-Ref Toxicant Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas 3 4 0.5510 0.5110 0.6270 0.6480 0.5780 0.6200 0.4530 0.5120 0.4050 0.3190 0.0000 0.0000 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean D-Control 0.5333 1.0000 0.5333 0.5070 0.5640 5.353 4 0.5703 450 0.6073 1.1388 0.6073 0.5760 0.6480 5.924 4 -2.307 2.180 0.0699 0.5703 600 0.5575 1.0455 0.5575 0.4690 0.6200 11.433 4 -0.756 2.180 0.0699 0.5575 750 0.4775 0.8955 0.4775 0.4530 0.5120 5.244 4 0.4775 900 0.3475 0.6517 0.3475 0.3100 0.4050 12.430 4 0.3475 1050 0.0523 0.0980 0.0523 0.0000 0.1580 142.558 4 0.0523 Tests Statistic Critical Skew Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94603 0.805 -0.6553 Bartlett's Test indicates egual variances (!;! = 0.40} 1.85289 9.21035 Test {1-tail, NOEC LOEC ChV TU MSDu MSB MSE F-Prob Dunnett's Test 600 >600 0.06992 0.13112 0.00569 0.00206 0.11571 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD iC05 629.55 44.05 IC10 683.02 39.17 IC15 736.48 32.27 IC20 774.58 14.39 IC25 807.48 14.81 IC40 902.72 14.24 IC50 931.69 9.08 0.7 0.6 0.5 ,1;; 0.4 (!) c . ,... 0.2 0.1 0 :g 0 10 c: 'V 8 0 95% 422.75 677.99 471.16 745.11 546.98 788.00 725.29 814.40 763.34 852.44 850.75 928.37 905.24 958.94 0 0 10 Skew -1.2511 -1.6458 -2.5024 0.0156 0.1502 -0.5101 0.1578 1.0 ......--------------. 0.9 0.8 0.7 0.6 0.5 &. 0.4 0.3 0.2 0.1 0.0...,..._- _ _.,_....., -0.1 -0.2 +--'lr--r-r-....-,.......--r-r-..,.......,.--...-.-....-,........j 0 500 1000 1500 Dose mg/L Dose-Response Plot 0 0 10 0 ,... Ol 0 10 1-tail, 0.05 level of significance N-Mean 1.0000 1.0000 0.9776 0.8374 0.6094 0.0916 Kurt 0.58729 df 2,9 File: ppkclcr_081412.xlsx Entered by: J. Sumner Reviewed

191:

'

(:;

- - - - I 1,. ;;;.* .. si Page4 of5 Species:

Pimephales promelas PpKCICR Test Number: 1.$S CONTROL 450 mgKCI/L 600 mgKCIIL 750 mgKCIIL 900 mgKCIIL STOCK *Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table Page Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: . . ' -. SOP A T21 -Exhibit A T21.1, revision 06-01-11 i *r**s.**** . ' . 6 Environmental Testing Solutions, Inc. Page 5 of5 Species: Pimephales promelas PpKCICR Test Number: -'2. SS CONTROL . 450 mg KCI/L 600 mgKCI/L 750 mgKCI/L 900 mgKCIIL II ' j* *Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Information table Page 1. Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: . ' SOP AT21-Exhibit AT21.1, revision 06-01-11 1.14 1.12 1.10 1.08 1.06 1.04 1.02 2.5 Q-u 2.0 = z 1.5 In M 1.0 u = 0.5 "= I t'-1.4 1.3 1.2 1.1 1.0 0.9 0.8 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart USEPA Control Limits (+/- 2 Standard Deviations)

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................... USEPA Warning and Control Limits (75th and 90th Percentile CVs) ...............................................................................................

. --.. * * * * * * * . ....... * * * * * * . ...... ...............................................................................................

Laboratory Warning and Control Limits (1oth and 25th Percentile CVs) *********************************************************************************************** -**-**-**--**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-

Test date

7-day IC25 = 25% inhibition concentration.

An estimation ofthe concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. --. Central Tendency (mean IC25) _ .. _ .. _ Warning Limits (mean IC25 +/- SA10 or SA75) .. * .. . . . . Control Limits (mean IC25 +/- S A25, S A90, or 2 Standard Deviations) Graphs generated from associated excel spreadsheet. Excel spreadsheet entered by: J. Sumner Reviewed Test number 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Test date 04-05-11 05-03-11 06-07-11 07-12-11 08-09-11 09-13-11 10-04-11 10-11-11 11-08-11 12-06-11 01-10-12 02-07-12 03-13-12 04-10-12 04-10-12 05-08-12 05-08-12 06-05-12 07-10-12 08-07-12 7-day IC25 (g/L NaCI) 1.07 1.07 1.05 1.06 1.06 1.06 1.07 1.06 1.05 1.06 1.08 1.08 1.08 1.07 1.07 1.06 1.05 1.05 1.07 1.08 CT (g/L NaCI) 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.06 1.07 1.07 1.07 1.06 1.06 1.06 1.06 1.06 1.06 1.06 s 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Ceriodaphnia dubio Chronic Reference Toxicant Control Chart State and USEPA Control Limits CT-25 CT+2S 1.05 1.05 1.05 1.04 1.04 1.04 1.04 1.05 1.05 1.04 1.04 1.04 1.04 1.05 1.05 1.05 1.05 1.05 1.05 1.05 1.09 1.09 1.09 1.09 1.09 1.09 1.09 1.08 1.08 1.08 1.09 1.09 1.09 1.08 1.08 1.08 1.08 1.08 1.08 1.08 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 Laboratory Warning Limits CT -SA.lo CT + SA.lo 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 1.16 1.16 1.16 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 SA.25 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 Laboratory Control Limits 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.24 1.24 1.24 1.24 1.24 1.25 SA.1s 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 USEPA Warning Limits 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.58 0.58 0.59 0.59 1.55 1.55 1.55 1.55 1.55 1.55 1.55 1.55 1.54 1.54 1.54 1.55 1.55 1.54 1.54 1.54 1.54 1.54 1.54 1.54 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 USEPA Control Limits CT -SA.9o CT + SA.90 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 1.73 1.73 1.73 1.73 1.73 1.73 1.73 1.73 1.73 1.72 1.73 1.73 1.73 1.72 1.72 1.72 1.72 1.72 1.72 1.72 cv 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Note: 7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC25). 5 = Standard deviation ofthe IC25 values. Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. SA.lo = Standard deviation corresponding to the 10th percentile CV. (SA.1o = 0.08) SA.25 = Standard deviation corresponding to the 25th percentile CV. (SA.25 = 0.17) USEPA Control and Warning Limits SA.75 = Standard deviation corresponding to the 75th percentile CV. (SA.7s = 0.45) SA.90 = Standard deviation corresponding to the 90th percentile CV. (SA.9o = 0.62) CV = Coefficient of variation of the IC25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, ancinnati, OH. File: CdNaCICR 080712.xlsx Entered by: J. Reviewed by: = Q ... 't = "0 e .... ......_; = ... .... g.. Q .... ...... Coi-i = Q Q .._, u ,-... = = Q = (J = "0 ** Q a. -c.. Q ...... e = ...... = .* Q (J = u -Q *cS u

40 35 30 25 20 15 40 30 20 10 50 40 30 20 10 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements
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.. **************************************************************************************** Minimum Acceptance Criteria(> 15.0 offspring per surviving female) North Carolina Acceptance Limit(< 40.0%) Kentucky Acceptance Limit(< 30.0%) USEPA Upper PMSD Bound (90th percentile< 47.0%) Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD) Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations) Graphs generated from associated excel spreadsheet. Excel spreadsheet entered by: J. Sumner Reviewed by:+

Precision of Endpoint Measurements
* -Ceriodaphnia dubio Chronic Reference Toxicant Data Environmental Testing Solutions, Inc. Test number 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Note: Test date Control Control Mean CT cv CT MSD PMSD Survival Reproduction CT for Control Mean for Control (%) (offspring/female)

Reproduction (%) Reproduction (%) forPMSD (%) (offspring/female) CV(%) 04-05-11 100 33.0 5.9 2.2 6.7 05-03-11 100 30.9 32.0 4.5 5.2 1.9 6.1 6.4 06-07-11 100 28.9 30.9 6.7 5.7 2.5 8.7 7.2 07-12-11 100 32.2 31.3 6.0 5.8 2.0 6.2 6.9 08-09-11 100 28.3 30.7 5.0 5.6 1.9 6.8 6.9 09-13-11 100 32.1 30.9 8.2 6.1 1.9 5.8 6.7 10-04-11 100 32.8 31.2 6.1 6.1 2.1 6.5 6.7 10-11-11 100 31.3 31.2 3.0 5.7 2.1 6.6 6.7 11-08-11 100 29.0 30.9 7.6 5.9 1.8 6.1 6.6 12-06-11 100 29.3 30.8 5.8 5.9 1.9 6.5 6.6 01-10-12 100 32.4 30.9 5.1 5.8 2.4 7.4 6.7 02-07-12 100 31.7 31.0 4.2 5.7 2.1 6.5 6.7 03-13-12 100 31.1 31.0 7.2 5.8 1.9 6.1 6.6 04-10-12 100 29.4 30.9 6.8 5.9 2.4 8.0 6.7 04-10-12 100 30.3 30.8 7.1 6.0 2.7 8.8 6.8 05-08-12 100 31.7 30.9 6.1 6.0 1.9 6.1 6.8 05-08-12 100 32.4 31.0 5.1 5.9 2.0 6.1 6.8 06-05-12 100 32.1 31.1 4.0 5.8 2.3 7.1 6.8 07-10-12 100 31.2 31.1 4.7 5.8 2.0 6.3 6.8 08-07-12 100 29.2 31.0 3.5 5.6 1.9 6.6 6.7 CV = Coefficient of variation for control reproduction. Lower CV bound determined by USEPA (lOth percentile)= 8.9%. Upper CV bound determined by USEPA (90th percentile)= 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (lOth percentile) = 13%. Upper PMSD bound determined by US EPA (90th percentile) = 47%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) US EPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. US EPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: CdNaClCR _ 080712.xlsx Entered by: J. Sumner Reviewed by:+ I 1"'***.**.* ... *.* ... *.****** I* Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Page 1 of6 . CdNaCICR

__;,;;l Dilution preparation information:

NaCl Stock INSS number: ""' Stock preparation: 100 g NaCl/L: Dissolve 50 g NaCl in 500 mL Milli-Q water. Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume_(mL) 1500 1500 1500 1500 1500 Test organism source information: Organism age: < 24-hours old Date and times organisms were born at*cn-*1-lObO between: Culture board: 0"\*&\*ll.... J\ Replicate number: I 2 1 3 1 4 1 s 1 6 1 1 1 s 1 9 10 Culture board cup number: \ 11.. I I I "\ 110 I ll I 1'\ I "2.0 ll't "5'1.. Transfer vessel information: pH =,.1r,1 S.U. Temperature = 1,\l,'l Average transfer volume (mL): o. .-..Q. .. .;. Daily renewal information: Day Date 0 2 3 4 5 6 7 Test initiation and feeding, renewal and feeding, or termination time oc Control information: Acceptance criteria % of Male Adults: 07. ::;;20% %Adults having 3rd Broods: f[)bl. ;?!:80% % Mortality:

07. ::;;20% Comments:

Test information: Randomizing template color: &ot.h Incubator number and shelf location: 1.1>\ YWTbatch: ()fo< tO.* *1..... Selenastrum batch: J"l .... MHSW. batch used Analyst Summary of test endpoints: 7-day LCso :>1&400 NOEC 1000 LOEC l2ntl Mean Offspring/Female: "2.1. 2. ;?!: 15.0 offspring/female ChV -s %CV: .. <40.0% IC2s 1011 .. 3 SOP AT14 -Exhibit A T14.1, revision 06-01-11 I ,, J ; I !ETS 0 En>tronmentaiTosUng Solutions, Inc. Species: Ceriodaphnia dubia CONTROL 1 Day ( 1 1 Young produced 0 Adult mortality \._... 2 Young produced t'l Adult mortality \._... 3 Young produced c Adult mortality \.._.. 4 Young produced .'-\ Adult mortality \...-5 Young produced \\ Adult mortality \..... 6 Young produced 0 Adult mortality L 7 Young );)roduced \S Total young produced Final Adult Mortality \-2 0 \...... Cl '-D. '-s L. D '-\D \..... -z.& '--X for 3rd Broods X. * )<... Page2 of6 _ CdNaCICR

Survival and Reproduction Data Replicate number 3 4 5 6 7 8 9 10 0 0 0 0 c 0 c 0 L.. L.. L-'--L L. L-\....._ c 0 c a 0 0 C) \_... L. L-L L-L... L '--a 0 a 0 Q a 0 n L. '--'--L \._ '--L.. '--t.( 4 s .5 \o '-\ s .s \.... '--\._ \.......

L.. \..._ L. \...... lO t() lC lt) \'2.. tt> tC '-\_ L '--\...... L '-\..... 0 () Q c .D a 0 0 \..... L. '--'--'--'--'--'--\S \S ' .... , . \S \'-=:. '1..4\ .. J>>\ 3b 7..9 -z-8 '-'---'---\,_ '--'--L.. '--><-)<. ')(: . ;><:. X Note: Adult mortaltty (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: % Mortality: I OJ. Mean Offspring/Female: I 600 mg NCIIL a S . I dR urvzva an d t* D t epro uc zon a a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced c 0 0 0 0 0 0 0 () 0 Adult mortality L. L L l L L L L L L 2 Young produced c 0 0 *o c (J 0 0 0 0 Adult mortality

t L L L L L L. L L L 3 Young produced

() a 0 0 0 {) *C 0 0 0 Adult mortality L L '-L L L. 'L \... \.._ L 4 Young produced 5 .s --{ \. to '"'\ '-s Adult mortality \... '-L \.._ L.. L L '--. \.._ '--5 Young produced \\ c l() \D l \ l \ 10 (() Adult mortality '-L L L \...... L L L L L 6 Young produced 0 1 \ 0 0 0 0 0 0 () 0 Adult mortality l-\...... \...... L L \.... L L L L.. 7 Young produced \'-\ '"' l'"\ -'""' Total young produced 3b C.. I\ '2.& 1."!) 1.f a'!t ,, Final Adult Mortality l.-L L L L L L '--. L-.___ Note: Adult mortahty (L = hve, D-dead), SB-&pltt brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer).

Concentration:

SOP AT14-Exhibit AT14.1, revision 06-01-11 iETS 6 EnvlronmentoiTostlngSolutlons.lnc. Page 3 of6 Species: Ceriodaphnia dubia CdNaCICR

\'!>8 800 N CI/L m2 a S . I dR urvzva an d t" D t epro uc zon a a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced a c 0 () 0 u 0 0 0 a Adult mortality

'-'-L '--L-L-L... L '--'--2 Young produced D 0 A I""\ {"\ t'\ (\ c 0 0 Adult mortality L \..... \.....-L.. L... '--'-.... '---'-... '--3 Young produced 0 D 0 0 0 0 c () 0 0 Adult mortality \..._ \._ '--\...._ L-L.. '--L-* '--\.._ 4 Young produced & S> '-\ '"\ 'l 5 '4 '-s Adult mortality L '--'-'-'--'---'--L... L..... 5 Young produced \\ \\ lC \\ \0 \'2.. IC \1.. IC Adult mortality L '-\....... L '--'--\...... 'L.. L-'--6 Young produced c D 0 b D a 0 0 (j () Adult mortality \.... \...... '--'-\..... \...... '--'\...._ L '--7 Young produced \'-\ \"'"\ 14 \'!> \\o 's \\ '\\..( Total young produced

o ,. "Z., "2.'\ '!.\ l.C\ Final Adult Mortality

'--\.. '-\...._ \..... L '-L. '-L Note: Adult momtlity (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: % Mortality:

07. Mean Offspring/Female:

30.\ % Reduction from Control: 1000 m2 NCIIL a S . I dR urvzva an d t* D t epro uc zon a a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 0 0 0 ('J (j Adult mortality \.._ L L L L L L L L L 2 Young produced 0 c a 0 a (J C) 0 c Adult mortality L L L L l L l L.... L L 3 Young produced 0 0 a (\ a (\ *(') (') 0 0 Adult mortality L L.. L L L L-L-L L-L 4 Young produced s '4. (o' \w\ '-\ \.\ '-\ '3 4 Adult mortality L L '--L L L l-L L \._ 5 Young produced 0 \L 1\ t\ tD 'b 10 10 10 10 Adult mortality L '-'-L. L L \.._ L... '-L 6 Young produced t\ 0 c D 0 0 c 0 0 t5 Adult mortality '-L \..... '-.. \.... \._. L \..., L 7 Young produced \5 'S \'!; 'S l'"Z.. \"\ \'-\' Total young produced !>>\ !.b 2C. 2.8 ,_, '-'-' . .,.., 'l.& Final Adult Mortality '--\..... L \. \. "--\.._ L L.. '-Note: Adult mortahty (L = hve, D =dead), SB = spht prood (smgle brood spht between two days), CO= carry over (offsprmg carried over with adult during transfer). Concentration: % Mortality:

01. Mean OffsprinWFemale:

28.'1 % Reduction .from Control:

z..17. SOP AT14-Exhibit AT14.1, revision 06-01-11 Page 4 of6 CdNaCICR#:

mg a s

I and Reproduction Data urv1va Replicate number Day 1 2 .3 4 5 6 7 8 9 10 1 Young produced Q 0 0 0 0 0 0 Q c 0 Adult mortality

\.._ \.... L-'--'--L.. L-\..._ \..._ L 2 Young produced c 0 c 0 *c c 0 0 0 tJ Adult mortality '-'--\._... L \..... \....... \,..... L. '--L_ 3 Young produced t) a () a a (') C) a (J () Adult mortality \.... L-'--\... \._ '--\..._ L .L '---4 Young produced \ L '2-.3. .2..* \ !. Adult mortality '-'-'--\..... \..... L L L '-\...... 5 Young produced ., 0 Cc '-\ s & s \. li s Adult mortality \ \..... '-L.. \........ L \.... \...._ '--L-6 Young produced a '\ 0 c () a D D c c Adult mortality L '--'--\........ L-'--L. '--'--"--7 Young produced '-l '--5 .s .,_ , '-\ S_ Total young produced I'-\ . '"' '\ 12.. \'!> \!:, \\:. (!, Final Adult Mortality '-'-'--'-:.. '-'-'--. '--\..._ '-Note: Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: % Mortality: 07 .. Mean Offspring/Female: % from Control: s'-\.2 1400 mg NCIIL a S . I dR urv1va an d t* D t epro uc zon a a Re_plicate number Day 1 2 3 4 5 '6 7 8 9 10 1 Young produced 0 Q 0 0 D {) D _0 0 0 Adult mortality \....... \...... L L L L L l-L L-2 Young produced 0 D 0 0 0 D c 0 0 _Q Adult mortality

L L. L L L L... L L L L-3 Young produced 0 0 0 0 () 0 '() 0 0 0 Adult mortality L L L L. L L L L. L... L 4 Young produced l \ \ '1.. 1_ 6 '2.. 3 \ Adult mortality L \..... L L L L L L L. L.. 5 Young produced 0 (:) '1.. ..3 (J '"1.. '"-\ \ '-. Adult mortality L L L \.......

\....... L L. \...-L.. L 6 Young produced ' 0 D "'L* 0 0 0 c 0 Adult mortality '-\.... '--'--\..._ \..._ \...... \...._ '--L. 7 Young produced -z... "L \ \ ' 3 1... \ Total young produced \, . '-\ \. '5 & ' ,. '.'-.. Final Adult Mortality \.. "' '--L. '--L L \..... '-Note: Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer).

Concentration:

emale: % Reduction from Control: SOP AT14 -Exhibit AT14.1, revision 06-01-11

* * * *
Environmental Testing Solutions, Inc. Control Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 4 5 4 5 11 0 10 6 0 10 0 7 15 13 15 Total 30 28 29 600 mg NaCIIL Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 5 5 4 5 11 0 10 6 0 11 0 7 14 13 14 Total 30 29 28 800 mg NaCI/L Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 5 5 4 5 11 11 10 6 0 0 0 7 14 14 14 Total 30 30 28 Replicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 4 5 5 6 10 10 10 12 0 0 0 0 15 14 15 13 29 29 30 31 Replicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 6 6 4 6 10 13 11 11 0 0 0 0 14 14 16 16 30 33 31 33 Replicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 11 10 12 10 0 0 0 0 13 13 16 15 28 27 33 29 Verification of Ceriodaphnia Reproduction Totals 1000 mg NaCI/L 8 9 10 Total Day 1 2 3 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 4 5 5 47 4 5 4 6 10 10 10 93 5 0 12 11 0 0 0 10 6 11 0 0 16 13 13 142 7 15 15 13 30 28 28 292 Total 31 31 30 1200 mg NaCIIL 8 9 10 Total Day 1 2 3 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 5 5 5 51 4 3 1 2 10 13 10 99 5 7 0 6 0 0 0 11 6 0 9 0 14 13 13 141 7 4 6 5 29 31 28 302 Total 14 16 13 1400 mg NaCI/L 8 9 10 Total Day 1 2 3 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 6 6 5 48 4 1 1 1 13 12 10 110 5 0 0 2 0 0 0 0 6 1 3 0 17 13 14 143 7 2 2 1 36 31 29 301 Total 4 6 4 Replicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 11 10 10 10 0 0 0 0 15 12 14 13 30 26 28 27 Replicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 2 3 2 1 4 5 8 5 0 0 0 0 3 5 2 7 9 13 12 13 Re})licate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 2 2 3 2 3 0 2 4 0 2 0 0 1 1 3 2 6 5 8 8 8 0 0 0 3 10 0 13 26 8 0 0 0 3 6 0 4 13 8 0 0 0 3 1 0 3 7 9 10 Total 0 0 0 0 0 0 0 0 0 4 4 42 10 10 94 0 0 11 13 14 137 27 28 284 9 10 Total 0 0 0 0 0 0 0 0 0 3 3 23 9 5 55 0 0 9 4 5 45 16 13 132 9 10 Total 0 0 0 0 0 0 0 0 0 3 1 19 2 2 16 0 0 6 1 3 19 6 6 60 File: CdNaCICR_080712.xlsx Entered by: J. Sumn(3r Reviewed by: -Jf-
* * * -Environmental Testing Solutions, Inc. Concentration (mg/LNaO) 1 2 3 Control 30 28 29 600 30 29 28 800 30 30 28 1000 31 31 30 1200 14 16 13 1400 4 6 4 Dunnett's MSD value: 1.929 PMSD: 6.6 Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control . Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR

138 Test dates: _____________

A_ug=-u_s_t_07_-_14_;,_2_0_12_ Replicate number Survival Average reproduction Coefficient of Percent reduction from (%) (offspring/female) variation (%) control (%) 4 5 6 7 8 9 10 29 29 30 31 30 28 28 100 29.2 3.5 Not applicable 30 33 31 33 29 31 28 100 30.2 6.0 -3.4 28 27 33 29 36 31 29 100 30.1 8.9 -3.1 30 26 28 27 26 27 28 100 28.4 6.9 2.7 9 13 12 13 13 16 13 100 13.2 15.1 54.8 6 5 8 8 7 6 6 100 6.0 23.6 79.5 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effiuent toxicity test. Lower PMSD bound determined by USEPA (lOth percentile)*= 13%. Upper PMSD bound determined by USEPA (90th percentile)= 47%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200la; USEPA, 2001b). USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effiuent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: CdNaCICR_080712.xlsx Table populated from associated Verification of Ceriodaphnia Reproduction Totals" spreadsheet. Spreadsheet entered by: J. Sumner Reviewed by:-<:/(-- Start Date: 8n12o12 End Date: 8/14/2012 Sample Date: Comments: Conc-mg/L 2 D-Control 30.000 28.000 600 30.000 29.000 800 30.000 30.000 1000 31.000 31.000 1200 14.000 16.000 1400 4.000 6.000 Conc-mg/L Mean N-Mean D-Control 29.200 1.0000 600 30.200 1:0342 800 30.100 1.0308 1000 28.400 0.9726 *1200 13.200 0.4521 *1400 6.000 0.2055 Auxiliary Tests Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Test ID: CdNaCICR Sample ID: Lab ID: ETS-Envir. Testing Sol. Sample Type: Protocol: FWCHR-EPA-821-R-02-013 Test Species: 3 4 5 6 7 8 29.000 29.000 29.000 30.000 31.000 30.000 28.000 30.000 33.000 31.000 33.000 29.000 28.000 28.000 27.000 33.000 29.000 36.000 30.000 30.000 26.000 28.000 27.000 26.000 13.000 9.000 13.000 12.000 13.000 13.000 4.000 6.000 5.000 8.000 8.000 7.000 Transform: Untransformed Rank Mean Min Max CV% N Sum 29.200 28.000 31.000 3.537 10 30.200 28.000 33.000 6.005 10 121.00 30.100 27.000 36.000 8.921 10 111.50 28.400 26.000 31.000 6.884 10 92.00 13.200 9.000 16.000 15.067 10 55.00 6.000 4.000 8.000 23.570 10 55.00 Statistic REF-Ref Toxicant NACL-Sodium chloride CD-Ceriodaphnia dubia 9 28.000 31.000 31.000 27.000 16.000 6.000 1-Tailed Critical 75.00 75.00 75.00 75.00 75.00 Critical 10 28.000 28.000 29.000 28.000 13.000 6.000 Isotonic Mean N-Mean 29.833 1.0000 29.833 1.0000 29.833 1.0000 28.400 0.9520 13.200 0.4425 6.000 0.2011 Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01) Bartlett's Test indicates equal variances Cp = 0.14) 1.17686 8.40619 1.035 15.0863 0.53863 0.89662 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 1000 1200 1095.45 Treatments vs D-Control Linear Interpolation (200 Resamples) Point IC05 IC10 IC15 IC20 IC25 IC40 IC50 mg/L 1000.77 1020.39 1040.02 1059.65 1079.28 1138.16 1177.41 SD 44.7051 9.61319 7.68096 7.19436 6.83735 6.71529 7.42545 95% CL Skew 868.009 1015.87 -1.6465 991.422 1034.38 -1.3975 1020.26 1053.25 -0.3510 1041.68 1071.64 -0.3336 1063.08 1090.45 -0.3157 1123.99 1148.74 -0.2616 1162.25 1189.63 -0.2048 1.0 .....------------ ..... 0.9 0.8 0.7 3! 0.6 s 0.5 Q. K! 0.4 0:: 0.3 0.2 0.1 0.0 .--. ........ -=--:-. ... 0 500 1000 1500 40 35 30 0 ts 25 :::J '8 20 .. Q. 15 10 5 0 :g c: 8 c Dose-Response Plot 0 0 0 0 0 0 0 0 co CX) C'll r 0 0 ..,. r Dose mg/L File: CdNaCICR_080712.xlsx Entered by: J. Sumner Reviewed Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 8/7/2012 8/14/2012 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mg/L D-Control 600 800 1000 1200 1400 Used for PMSD calculation only. 1 2 3 4 30.000 28.000 29.000 29.000 30.000 29.000 28.000 30.000 30.000 30.000 28.000 28.000 31.000 31.000 30.000 30.000 14.000 16.000 13.000 9.000 4.000 6.000 4.000 6.000 5 29.000 33.000 27.000 26.000 13.000 5.000 6 30.000 31.000 33.000 28.000 12.000 8.000 Transform: Untransformed Conc-mg/L Mean N-Mean Mean Min Max D-Control 29.200 1.0000 29.200 28.000 31.000 600 30.200 1.0342 30.200 28.000 33.000 800 30.100 1.0308 30.100 27.000 36.000 1000 28.400 0.9726 28.400 26.000 31.000 *1200 13.200 0.4521 13.200 9.000 16.000 *1400 6.000 0.2055 6.000 4.000 8.000 AuxiliaryTests Kolmogorov D Test indicates non-normal distribution (p <= 0.01) Bartlett's Test indicates egual variances (p = 0.14) Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV Dunnett's Test 1000 1200 1095.45 Treatments vs D-Control CV% 3.537 6.005 8.921 6.884 15.067 23.570 TU 7 31.000 33.000 29.000 27.000 13.000 8.000 N 10 10 10 10 10 10 Statistic 1.17686 8.40619 8 30.000 29.000 36.000 26.000 13.000 7.000 t-Stat -1.186 -1.067 0.948 18.969 27.505 MSDu MSDp 1.92879 0.06605 9 28.000 31.000 31.000 27.000 16.000 6.000 1-Tailed Critical 2.287 2.287 2.287 2.287 2.287 Critical 1.035 15.0863 MSB 1109.51 10 28.000 28.000 29.000 28.000 13.000 6.000 MSD 1.929 1.929 1.929 1.929 1.929 Skew Kurt 0.53863 0.89662 MSE F-Prob df 3.55741 1.6E-38 5, 54 File: CdNaCICR_080712.xlsx Entered by: J. Sumner Reviewed by: 4 I i I :* .. t:**.*. '. I' Species: Ceriodaphnia dubia CdNaCICR#: CONTROL 600 mg NaCI/L 800 mg NaCI!L 1200 mg NaCIIL STOCK *Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: 6'{ . . Page 5 of6 SOP AT14-Exhibit AT14.1, revision 06-01-11 Species: Ceriodaphnia dubia CdNaCICR#: CONTROL 600 mg NaCIIL 800 mg NaCIIL 1200 mg NaCIIL 1400 mg NaCIIL *Temperatures performed at the time oftest initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: d( . Page 6 of6 SOP AT14-Exhibit AT14.1, revision 06-01-11 S58 12111 0 800 -NPDES CORRESPONDENCE November 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SON)-NPDES PERMIT NO. TN0026450-DISCHARGE MONITORING REPORT (DMR) FOR OCTOBER 2012 Enclosed is the October 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The turbine building sump (TBS) discharged directly to the yard drainage pond during the reporting period and was monitored in accordance with the narrative condition found in Part 1.A.2. of the subject permit. Enclosed is TBS monitoring data as well as attachments describing two collection system overflows that occurred during the reporting period. At no time was there a threat to public drinking

supplies, to human health, or the environment.

If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Signatory Authority for: John T. Carlin Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 S.D. Booker, MOB 1 F-WBN B. E. Brickhouse, BR 4A-C J. T. Carlin, OPS 4A-SQN J. A. Cross, POB 2A-SQN T.R. Markum, BR 4A-C D. B. Nida, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 J.W. Proffitt, OPS 4C-SQN A. A. Ray, WT 11A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1 E-M Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 November 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-DISCHARGE MONITORING REPORT (DMR) FOR OCTOBER 2012 Enclosed is the October 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The turbine building sump (TBS) discharged directly to the yard drainage pond during the reporting period and was monitored in accordance with the narrative condition found in Part 1.A.2. of the subject permit. Enclosed is TBS monitoring data as well as attachments describing two collection system overflows that occurred during the reporting period. At no time was there a threat to public drinking

supplies, to human health, or the environment.

If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Plant Manager Signatory Authority for: John T. Carlin Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 TV A Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 1 Description of the Event and Determination of Cause On 1 0/19/2012 at 04:25 EDT the Sequoyah (SQN) Shift Manager was notified of a sewage overflow coming from a manhole located on the west side of the SQN Cafeteria. The shift manager initiated the SQN Spill Plan and dispatched personnel to investigate. A slight stream of sewage was discovered flowing out of the manhole ancl running approximately 50 feet down an access road before it reached a yard drain. On 10/19/2012 at 04:40 EDT the sewage overflow was stopped by cycling the number two pump from auto to manual and pumping the manhole down. Preliminary information regarding the overflow was communicated by telephone to the Chattanooga Environmental Field Office on 10119/2012 at 15:03 EDT. The total quantity of sewage which overflowed into a yard drain was estimated at less than 5 gallons. The initial investigation did not reveal any interruptions in power which could have caused the number two pump to trip in the off position. The cause of the overflow was attributed to partial clogging of the number two pump. This manhole is also where the cafeteria grey water enters the sewer system. At this time SQN is in a refueling outage and the partial clogging of the number two pump was caused by increased usage of the sewer system by support personnel and continuous cafeteria usage. All remaining sewage residue in the access road was neutralized with lime. The yard drain flows to the SQN Yard Drainage Pond, which discharges to the Diffuser Pond. The Diffuser Pond discharges through Outfall 1 01 to the Tennessee River. At no time was there a threat to public drinking

supplies, to human health, or the environment.

Period of Discharge The shift manager was notified of the overflow on 10/19/2012 at 04:25 EDT. The sewage overflow had just reached the yard drain as personnel responding to the spill arrived. The number two sewage ejector pump was restarted on 1 0/19/2012 at 04:40 EDT and this action immediately stopped the overflow. and discharge to the yard drain. The estimated time of discharge was less than 15 minutes. Steps Being Ta.ken to Reduce, Eliminate, and Recurrence This incident was entered into the TVA Corrective Action Program. Sequoyah has implemented shiftly monitoring of this sewage ejector location as an added precaution for the remaining duration of the outage to prevent another overflow. TV A Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 2 Description of Event and Determination of Cause On 10/27/2012 at 23:00 EDT Sequoyah (SQN) Shift Manager was notified of a high level alarm and a small amount of water coming out of the control panel for the turbine building sewage ejector pump controls. The shift manager initiated the SQN Spill Plan and dispatched personnel to investigate.

On 10/27/2012 at 23:15 EDT a Senior Reactor Operator (SRO) responded to the sewage ejector control panel which did not have any lights indicating sewage ejector pump operation.

The sewage water had filled the electrical conduit supplying power to the ejector pumps and was trickling out of the sewage ejector control panel, running down the building and into a floor drain. The SRO reset the sewage ejector control panel and the pumps restarted. On 10/27/2012 at 23:30 EDT all sewer water leakage was isolated from the control panel. The total estimated quantity of sewer water which reached the floor drain was estimated at less than 1 gallon. The floor drain flows into the turbine building sump. The turbine building sump is discharged to the Low Volume Waste Treatment Pond which discharges through Internal Monitoring Point 1 03 to the Diffuser Pond. The Diffuser Pond discharges through Outfall 101 to the Tennessee River. At no time was there a threat to public drinking

supplies, to human health, or the environment.

Period of Discharge The shift manager was notified of the high level alarm and a small amount of water flowing out of the sewage ejector pump control box on 10/27/2012 at 23:00 EDT. The sewer water was running out of the electrical control panel, traveling down the building wall, and entering the floor drain The sewage ejector control box was reset and the pumps restarted. The sewer water flowing out of the control panel was stopped on 10/27/2012 at 23:30 EDT. The estimated time of discharge is approximately 30 minutes. Steps Being Taken to Reduce, Eliminate, and Prevent Recurrence This incident was entered into the TVA Corrective Action Program. An investigation is ongoing to determine the exact cause of the loss of power at the turbine building sewage ejector control panel. Sequoyah has implemented. shiftly monitoring of this sewage ejector location as an added precaution until the apparent cause has been identified and repaired to prevent another overflow. An update on the apparent cause will be provided in the November DMR submittal. TV A Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 3 The turbine building sump was discharged directly to the yard drainage pond on 10/24/2012 and 10/28/2012-10/31/2012. During this period, the turbine building sump was monitored in accordance with the narrative condition found in Part 1.A.2 of NPDES Permit TN0026450. Turbine Building Sump Monitoring Data Parameter Daily Minimum Monthly Average Daily Maximum No. of Samples Flow -1.525 MGD 2.580 MGD 5 pH 8 s.u. -9 s.u. 5 O&G -< 5 mg/L < 6 mg/L 5 TSS -7 mg/L 10 mg/L 5 PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different} _Mdres!,_ _f.Q,_BOX....2.POO

__ -__ _§ODDY- ________ _ A TIN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT

- - NO DISCHARGE D *** Form Approved.

OMB No. 2040-0004 NOTE: Read instructions before completina this fonn PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ********

36.0 0 31/31 RCORDR ** 04 CENTIGRADE 00010 1 0 PERMIT ********

-********

Req *. Mon. DEG.C. CONTI CALC TO EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE ********

25.4 0 31/31 MODELD ** 04 CENTIGRADE 00010 z 0 PERMIT. ********

-**** -********

30.5 DEG.C. CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAILYMX* NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ********

3 0 31/31 CALCTD ** 04 UPSTRM DEG.C MEASUREMENT 00016 1 s PERMIT ********

-********

3 DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILYMX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** 1755 ********

0 31/*31 RCORDR 03 ** 50050 1 0 PERMIT ******** Req. Mon. MGD ********

-CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ********

0.015 0.059 0 23/31 GRAB ** 19 50060 1 0 PERMIT ********

-******** 0.1 0.1 MGIL FIVE PER CALCTD EFFLUENT GROSS REQUIREMENT MOAVG DAILY MAX WEEK TEMPERATURE* C, RATE OF SAMPLE ******** 0 ********

0 31/31 CALCTD 62 ** CHANGE 82234 1 0 PERMIT ******** 2 DEG * ********

- - - - -CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILYMX C/HR NUOUS SAMPLE PERMIT REQUIREMENT II NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I. Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Paul R. Simmons properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage tile system, or those persons directly responsible for gathering the 423 843-6502 12 Information, tho information submitted is , to the best of my knowledge and belief, true, a=rate, Sequoyah Plant Manager and complete. I am aware that there ara significant penalties for submitting falsa information, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER TYPED OR PRINTED CODE COMMENTS AND OF ANY (Reference all attachments here) No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred:

1. Floguard MS6236 (max. calc. cone. was 0.029mg/L-Iimit 0.2mg/L)

2. Biodetergent 73551 (max .. calc. cone. was 0.038mg/L-Iimit 2."0mg/L)

3. Spectrus CT1300 (max. calc. cone. was 0.036mg/L-Iimit 0.050mg/L)

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 11 10 MO DAY -Mean# of Water Mean# of Water NOTES:% Sample Date ZM/m3 %Settlers Temp.("C) Sample Date Asiatic Temp.fC) LOCATION SUB LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW W8E 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 P8 01/24/2012 o* 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 88 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5. 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 W8E 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 W8E 03113/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 W8E 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 W8E 03/27/2012 1623 . 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 W8E 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 P8 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 P8 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May2012 No Samples Collected June 2012 No Samples Collected July 2012 No Samples Collected August 2012 No Samples Collected September 2012 No Samples Collected 10/23/2012 394 8 17 10/23/2012 82 17 1-ISV-24-1234 WAW PERMilTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) Form Approved. OMB No. 2040-0004 Address P.O. BOX 2000 = = = .J!..NTEROFFICE OPS-5N-SQID=-= = = = = = ____ _ ATTN: Brad Love PARAMETER X IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 PERMIT EFFLUENT GROSS REQUIREMENT IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 PERMIT EFFLUENT GROSS REQUIREMENT SAMPLE PERMIT REQUIREMENT . SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT PERMIT REQUIREMENT PERMIT REQUIREMENT QUANTITY OR LOADING AVERAGE MAXIMUM ********

UNITS MINIMUM.

- Monitoring Not Required

-43.2 MINIMUM ** Monitoring Not Required -43.2 MIMINUM F-FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT

- - NO DISCHARGE D *** NOTE: Read instructions before completin!l this form QUALITY OR CONCENTRATION NO. FREQUENCY EX OF AVERAGE MAXIMUM UNITS ANALYSIS

23 ********"

PERCENT SEMI ANNUAL ********

23 ********

PERCENT SEMI ANNUAL SAMPLE TYPE COMPOS COMPOS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of Jaw that this document and ell attechments were prepared under my TELEPHONE DATE diredion or supervision In acccirdance with a system designed to assure that qualified personnel Paul R. Simmons properly gather and evaluate the information.submilled. Based on my inquiry of the person or persons who manage the system, or those persons diredly responsible for gathering the Information, the information submitted is , to the best of my knowledge and belief, true, accurate, Sequoyah Plant Manager and complete. I am awere that there are significant penalties for submitting false information, including the possibility of line and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY (Reference all attachments here) Toxicity was not sampled in October 2012. EPA Form 3320-1 (REV 3199) Previous editions may be used Plant Manager SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 843-6502 12 11 10 I AREA I NUMBER MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres!,__E.O. BOX2000 NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT" (DMR) TN0026450 MAJOR (SUBR 01) F -FINAL Fonn Approved. OMB No. 2040-0004 _______ _ PERMIT NUMBER LOW VOL. WASTE TREATMENT POND _____ _ EFFLUENT A TIN: Brad Love *** NO DISCHARGE D *** NOTE: Read instructions before complelln!l this fonn PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ********

7 ******** 8 0 19/31 GRAB -12 00400 1 0 PERMIT ........ ..... _ -6 ........ 9 su THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK

SOLIDS, TOTAL SUSPENDED SAMPLE ********

9 13 0 2/31 GRAB ** 19 00530 1 0 PERMIT --*--*--********

- *---****

30 100 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH OIL AND SAMPLE ********

<6 <6 0 2/31 GRAB -19 00556 1 0 PERMIT -**--** ........

- ---*** 15 20 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX*

MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.242 1.834 ********

--* 0 31/31 RCORDR 03 - PLANT 50050 1 0 PERMIT Req *. Mon. Req. Mon MGD *----***

----*--........

.. SEE RCORDR

EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT

-NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared undar my TELEPHONE; DATE direction or supervision in accordance with a system designed to assure that qualified personnel Paul R. Simmons property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manege the system, or those persons directly responsible for gathering the 423 I 843-6502 12 11 10 Sequoyah Plant Manager Information, the information submitted is , to the best of my knowiedge and belief, true, accurate, and complate. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of line and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER MO DAY TYPED OR PRINTED CODE COMMENTS AND OF ANY (Reference all attachments here) EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 NATIONAL POlllJTANT DISCHARGE EUMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRI TN0026450 110 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 20-40-0004 PERMIT NUMBER RECYCLED COOLING WATER EFFLUENT ATTN: Brad love .... NO DISCHARGE I XX I .... NOTE: Read instructions before completing lhis form PARAMETER QUANllTY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE __ ANAlYSIS !TEMPERATURE , WATER DEG. CENTIGRADE 0!)010 1 0 EFFLUENT GROSS VALUE TEMPERATURE, WATER DEG. CENTIGRADE 00010 z 0 INSTREAM MONITORING !TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL 50060 0 EFFLUENT GROSS VALUE TEMPERATURE-C, RATE OF CHANGE 82234 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMEN T PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT -**'******

"*******

...........

- - - - '**

...........

-Req. Mon. DAILY MX ......... 2 DAILYMX -03 MGD 04 DEGC ............ ............ --****'**** ...........

- - - - '* ..............

.........

--0.1 MOAVG . /7 REPORT DAILYMX 30.5 DAILYMX 5 DAILYMX ******** 0.1 DAILYMX ---NAMEITITL E PRINCIPAL EXECUTIVE OFFICER Paul R. Simmons Sequoyah Plant Manager I Certify tRier penalty of law lhallhis document and all attachments were prepared under my cf.-edicn or supeMsion in acccrt1ance wllh a syslem dasigned to assure thai qualified personnel property galher and evaluate the intormalion submitted. Based en my inquiry clthe person ot ( persons wno manage the system. or lhose persons diredly responsible for gathenng the S quoyah Plant Manager lr1klrmabcn , the submrtled is , to lhe best cl my knowledge and belief, true, ac::aKale . anct complete_ 1 am ewanolhat 1t1ere are significanl pena!lles tor submitting false infonnatJon , SIGNATURE OF PRINCIPAL EXECUTIVE 1-----==------------l including the possibility cl fine and imprisorvnent lor knowvlg YIOiabons. OFFICER OR ALITHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here} No Discharge this Period EPA Form 3320*1 (REV 3/!r.l) Previous editions may be *used 04 DEGC 04 OEGC 04 OEGC -19 MG/L -TELEPHONE 423 I 843-6502 AREA I NUMBER CODE CONTIN CALCTD uous CONTIN CALCTD uous CONTIN CALCTD uous CONTIN RCORDR uous Frve per CALCTD Week CONTIN CALCTD uous DATE 12 11 10 YEAR MO DAY Page 1 of 1 PERMmEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 204()-()004 Addres.L _e.Q._BOX 2000 ___________ _ ___ .J!.NTEROFFICE OPS-5N-SQID _______ _ TN0026450 PERMIT NUMBER ____ _ ATTN: Brad Love PARAMETER >< QUANTITY OR LOADING AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE '********

-CERIODAPHNIA TRP3B 1 0 0 PERMIT ******** ........ -EFFLUENT GROSS VALUE REQUIREMENT IC25 STATRE 7DAY CHR SAMPLE ********

-PIMEPHALES TRP6C 1 0 0 PERMIT ********

******* -EFFLUENT GROSS VALUE REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT.

SAMPLE MEASUREMENT PERMIT. REQUIREMENT MINIMUM 43.2 MINIMUM 43.2 . MINIMUM RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE I XX I *** NOTE: Read instructions before completinQ this fonn QUALITY OR CONCENTRATION NO. FREQUENCY EX OF AVERAGE MAXIMUM UNITS ANALYSIS
    - - '**

23 * ******* ........ PERCENT SEMI ANNUAL ********

23 **"**** *****"* PERCENT SEMI ANNUAL ......-=- SAMPLE TYPE COMPOS COMPOS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of Jaw that this document and all attachments were prepared under my ..... TELEPHONE DATE Paul R. Simmons direction or supervision In accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Plant Manager Information, the Information submitted is , to the bast of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT -423 843-6502 12 11 10 I AREA I NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} Name TVA-SEQUOYAH NUCLEAR PLANT NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} DISCHARGE MONITORING REPORT (DMR} MAJOR (SUBR 01) F -FINAL Fonn Approved. OMB No. 2040-0004 Address P.O. BOX2000 ============ TN0026450 ____ _ ATTN: Brad Love PARAMETER X QUANTITY OR LOADING AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO)

"* 00300 1 0 PERMIT ******** . ....... -MINIMUM 2 118 G WASTEWATER & STORM WATER EFFLUENT

- - NO DISCHARGE I XX I *** NOTE: Read Instructions before complellnR this fonn QUALITY OR CONCENTRATION NO. FREQUENcy EX OF AVERAGE MAXIMUM UNITS ANALYSIS

19 ........ --MGIL TWICE/ SAMPLE TYPE GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED

"* 19 00530 1 0 PERMIT ******** -**** "*** ********

100 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX WEEK SOLIDS, SETTLEABLE

"* 25 00545 1 0 PERMIT ........

-******** ........ 1 MUL ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX MONTH FLOW, IN CONDUIT OR THRU

03 "* TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD * ******* -----........

ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX BATCH PERMIT REQUIREMENT PERMIT REQUIREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER.

I Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Paul R. Simmons properly gather and evaluate the information submitted. Based on my Inquiry of the person or parsons who manage the system, or those parsons directly responsible for gathering the 423 843-6502 12 11 information, the information submitted is , to the best of my knowledge and belief, true, accurate. 10 Sequoyah Plant Manager and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREAl NUMBER MO DAY TYPED OR PRimED CODE COMMENTS AND EXPLANATION OF ANY (Reference all attachments here) During this reporting period, there has been no fiQw from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-October 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 11/09/2012 CONCURRENCES Name R c Signature -Comment Date v N - A\.._ "'""z.... B.M. Love X ---, ICL----' " L.M. Koby \ l X '---> \ \i J iDj t2--J.A. Cross X *,,.) '/c'AU.t., 01 I I illn*/1 d-'* INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpo$e, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. S58 121214 800-NPDES CORRESPONDENCE December 14, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-DISCHARGE MONITORING REPORT (DMR) FOR NOVEMBER 2012 Enclosed is the November 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The turbine building sump (TBS) discharged directly to the yard drainage pond during the reporting period and was monitored in accordance with the narrative condition found in Part 1.A.2. of the subject permit. Also enclosed are two attachments containing information regarding a collection system overflow that occurred during the reporting period, TBS monitoring data, and an update for previously reported collection system overflows . At no time has there been any observed threat to public drinking supplies, to human health, or the environment. If you have any questions or need additional information , please contact Brad Love by email at bmlove@tva .gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information , the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information , including the possibility of fine and imprisonment for knowing violations . Enclosures cc (Enclosures)

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D. Booker, MOB 1 F-WBN B. E. Brickhouse

, BR 4A-C J. T. Carlin, OPS 4A-SQN J. A. Cross, POB 2A-SQN T.R. Markum, BR 4A-C D. B. Nida, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington , DC 20555 J.W. Proffitt, OPS 4C-SQN A. A. Ray, WT 11A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1 E-M Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 December 14, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-DISCHARGE MONITORING REPORT (DMR) FOR NOVEMBER 2012 Enclosed is the November 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The turbine building sump (TBS) discharged directly to the yard drainage pond during the reporting period and was monitored in accordance with the narrative condition found in Part 1.A.2. of the subject permit. Also enclosed are two attachments containing information regarding a collection system overflow that occurred during the reporting period, TBS monitoring data, and an update for previously reported collection system overflows. At no time has there been any observed threat to public drinking

supplies, to human health, or the environment.

If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations . . '*Carlin §" e 1ce President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 TVA Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 1 Description of the Event and Determination of Cause On 11/25/2012 at 22:13 EDT the Sequoyah (SQN) Shift Manager was notified of a low-flow sewage overflow coming from a manhole located in the roadway between the Plant Office Building, Multi-Purpose

Building, and Security Building.

The shift manager initiated the SQN Spill Plan and dispatched personnel to investigate. An intermittent stream of sewage was discovered flowing out of the manhole and running approximately 20 feet across the road before it reached a yard drain. On 11/25/2012 at 22:30 EDT the sewage overflow was stopped from entering the yard drain. All remaining sewage residue in the road was neutralized with lime. The yard drain flows to the SQN Yard Drainage Pond, which discharges to the Diffuser Pond. The Diffuser Pond discharges through Outfall101 to the Tennessee River. At no time was there any observed threat to the public drinking

supplies, to human health, or the environment.

The total quantity of sewage that overflowed into a yard drain was estimated to be less than 5 gallons.

The overflow was caused by a mass of foreign material becoming entangled around the ejector pump float switch. The ejector pump float switch became locked in the high level position and the pump continued to run. The ejector pump eventually tripped du.e to overheating which led to an associated trip of. the high level alarm. Period of Discharge The shift manager was notified of the overflow on 11/25/2012 at 22:13 EDT. The sewage overflow was entering the yard drain as personnel responding to the spill arrived. The sewage was stopped from entering the yard drain on 11/25/2012 at 22:30 EDT. The estimated time of discharge was approximately 17 minutes. Steps Being Taken to Reduce, Eliminate, and Prevent Recurrence This incident was entered into the TVA Corrective Action Program. Sequoyah continues daily monitoring of sewage ejector pump locations to ensure control power has not been isolated and has added visual inspections of associated manholes to verify the absence of foreign material which may cause ejector pump issues. TV A Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 2 Turbine Building Sump Monitoring Data The turbine building sump was discharged directly to the yard drainage pond from 11/10/2012 to 11/15/2012. During this period, the turbine building sump was monitored in accordance with the narrative condition found in Part 1.A.2 of NPDES Permit TN0026450. Parameter Daily Minimum Monthly Average Daily Maximum No. of Samples Flow -0.480 MGD 1.150 MDG e pH 8 s.u. -8 s.u. 5 O&G -< 4 mg/L < 6 mg/L 5 TSS -4 mg/L 7 mg/L 5 Update on Previously Reported Sewage Collection System Overflows SQN is herein providing an update regarding sewage collection system overflow events previously reported to the Division. Each event was entered into the TVA Corrective Action Program, and an investigation was initiated to determine the cause(s). Throughout the investigation and the duration of the Steam Generator Replacement outage, Sequoyah has implemented shiftly monitoring of onsite sewage ejectors as an added precaution. SQN determined the sewage collection system overflows had two apparent causes. The . investigation. indicated there was no consistent method to inform secondary and tertiary electrical-feeds when switching circuit breakers of primary electrical systems. The investigation also indicated there are potential opportunities to improve the inspection program. As a result, TVA will elicit the involvement of a Grade I collection system operator to review the existing system and provide recommendations for necessary upgrades to the material condition and inspection program. The review will include at a minimum an evaluation of current aiarm system design and inspection program scope and frequency. In addition, measures will be taken by site personnel to improve response awareness and ensure prompt _and appropriate actions continue to be taken. PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Nar:rut _ _!_VA-SEQUOYAH NUCLEAR PLANT _ _ _ _ . NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMS No. 2040-0004 AddreS.L ....f.Q._BOX 2000 ___________ _ TN0026450 ____ _ ATTN: Brad Love PARAMETER X QUANTITY OR LOADING AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ********

- - '*****

-CENTIGRADE 00010 1 0 PERMIT ********

-EFFLUENT GROSS REQUIREMENT

-TEMPERATURE, WATER DEG. SAMPLE ********
- CENTIGRADE 00010 z 0 PERMIT ********

-INSTREAM MONITORING REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & SAMPLE ********

-UPSTRM DEG.C 00016 1 1 PERMIT ********

-EFFLUENT GROSS REQUIREMENT FLOW, IN CONDUIT OR THRli SAMPLE ******** 983 03 TREATMENT PLANT 50050 1 0 PERMIT *****"** Req. Mon. MGD EFFLUENT GROSS REQUIREMENT DAILY MAX CHLORINE, TOTAL RESIDUAL SAMPLE ********

-. 50060 1 0 PERMIT ******** ........ -EFFLUENT GROSS REQUIREMENT TEMPERATURE-C, RATE OF SAMPLE ******** 0 62 CHANGE 82234 1 0 PERMIT ******** 2 DEG EFFLUENT GROSS REQUIREMENT DAILYMX C/HR SAMPLE PERMIT MINIMUM ********

'********

DIFFUSER DISCHARGE EFFLUENT

- - NO DISCHARGE NOTE: Read instructions before completina this form QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS

29.6 0 30/30 RCORDR 04 ******** Req. Mon. DEG.C. CONTI CALCTD DAILY MAX NUOUS ******** 17.4 0 30/30 . MODELD 04 ******** 30.5 DEG.C. CONTI CALCTD DAILYMX NUOUS* ******** 2 0 30/30 CALCTD 04 ******** 5 DEG.C. CONTI CALC TO DAILYMX NUOUS ********

0 30/30 RCORDR ** ........

******* .... CONTI RCORDR NUOUS 0.012 0.020 19 0 14/30 GRAB 0.1 0.1 MG/L FIVE PER CALCTD MOAVG DAILY MAX WEEK ********

0 30/30 CALCTD -********

- - - - .... CONTI CALCTD NUOUS REQUIREMENT

-?-/:i;. /} NAMEITIT.LE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE John T. Carlin direction or supervision in accordance with a system designed to assure the! qualified parsonnel( proparly gather and evaluate the infonnalion submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Site Vice President infonnalion, the*lnfonnation submitted is , to the best of my knowledge and belief, true, accurate, . Vtl:e" 423 843-7001 12 12 14 and complete. I am aware that there ere significant panallies for submitting false infonnation, PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. AREA I ER OR AUTHORIZED AGENT NUMBER MO DAY TYPED OR PRINTED CODE COMMENTS AND OF ANY TIONS (Reference all attachments here) No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred:

1. Floguard MS6236 (max. calc cone. was 0.1 03mg/L-Iimit 0.2mg/L),

2. Biodetergent 73551 (max. calc. cone. was 0.051 mg/L-Iimit 2.0mg/L)

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 Mean# of Water Mean# of Water NOTES:% Sample Date ZM/m3 %Settlers Temp. ("C) Sample Date Asiatic Temp. ("C) LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 . 0 10 PB 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE 03/13/2012 0 0 13 03/13/2012 o* 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 PB 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 PB 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May 2012 No Samples Collected June 2012 No Samples Collected July 2012 No Samples Collected August2012 No Samples Collected September 2012 No Samples Collected 10/23/2012 394 8 17 10/23/2012 82 17 1-ISV-24-1234 WAW 10/30/2012 34 50 17 10/30/2012 17 17 1-ISV-24-1234 WAW 11/06/2012 0 0 17 11/06/2012 0 17 1-ISV-24-1234 WAW 11/13/2012 0 0 15 11/13/2012 0 15 1-ISV-24-1234 WAW 11/20/2012 0 0 13 11/20/2012 0 13 1-ISV-24-1234 WAW 11/28/2012 0 0 12 11/28/2012 0 1-ISV-24-1234 WAW 12/04/2012 0 0 12 12/04/2012 0 12 1-ISV-24-1234 WAW PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Addres!.__E.O. BOX2000 NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR01) F -FINAL Fonn Approved. OMB No. 2040-0004 _______ _ ____ _ BIOMONITORING FOR OUTFALL 101 EFFLUENT ATTN: Brad Love *** NO DISCHARGE D *** NOTE* Read instructions before completin!l this fonn PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRAnON AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM IC25 STATRE 7DAY CHR SAMPLE ********

Monitoring

- - '*****

... CERIODAPHNIA Not Required TRP3B 1 0 PERMIT ********

-43.2 ********

- - - - <<!

EFFLUENT GROSS REQUIREMENT MINIMUM SAMPLE ******** IC25 STATRE 7DAY CHR ********

Monitoring

... PIMEPHALES Not Required TRP6C

1 0 PERMIT ********

-43.2 ********

EFFLUENT GROSS REQUIREMENT MIMINUM SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT / ( ./J .** NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of Jaw that this document and all allachmants were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , *to the bast of my knowledge and belief, true, accurate, Site Vice President and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisoAment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY TIONS (Reference all attachments here) Toxicity was not sampled in November 2012. EPA Fonn 3320-1 (REV 3199) Previous editions may be used I 'L-;. .. --*-T restdent Slef[ATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 23 PERCENT SEMI COMPOS ANNUAL --23 PERCENT SEMI COMPOS ANNUAL TELEPHONE DATE 423 843-7001 12 12 14 I AREA I NUMBER MO DAY CODE Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different} ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} DISCHARGE MONITORING REPORT (DMR} MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Addres.!_ ...f.O. BOX 2000 _____ --' _____ _ LOW VOL. WASTE TREATMENT POND ____ _ EFFLUENT A TIN: Brad Love *** NO DISCHARGE D *** NOTE: Read instructions before completin!l this form PARAMETER l>< QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ******** 7 ******** 8 0 13/30 GRAB -12 00400 1 0 PERMIT ******** ........ -6 ******** 9 su THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK -*-SAMPLE ******** SOLIDS, TOTAL SUSPENDED

8 10 0 2/30 GRAB -19 00530 1 0 PERMIT ********

-******** 30 100 MGIL lWICEI GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH OIL AND GREASE SAMPLE ********

<6 <6 0 2/30 GRAB -19 00556 1 0 PERMIT ********

-******** 15 20 MGIL lWICEI GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.254 1.685 ********

0 30/30 RCORDR 03 - PLANT 50050 1 0 PERMIT Req. Mon. Req.Mon MGD ********

.. SEE RCORDR EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX PERMIT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT / -n/1 ./7 NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my 'r1ffP TELEPHONE DATE direction or supervision in accordance with. a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for galharing tha / 423 Site Vice President information, tha information submitted is , to tha bast of my knowiedga and ballet, true, accurate, 843-7001 12 12 14 and complete. I am aware that there are significant penalties for submitting false information, PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER MO DAY TYPED OR PRINTED CODE COMMENTS AND OF ANY TIONS (Reference all attachments here) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Addres.!__E..O. BOX2000 ____ _ A TIN: Brad Love PARAMETER >< TEMPERATURE, WATER DEG. SAMPLE CENTIGRADE 00010 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE, WATER DEG. SAMPLE CENTIGRADE 00010 z 0 PERMIT INSTREAM MONITORING REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & SAMPLE UPSTRM DEG.C 00016 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT 50050 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT

CHLORINE, TOTAL RESIDUAL SAMPLE 50060 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE-C, RATE OF SAMPLE CHANGE 82234 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT SAMPLE PERMIT . REQUIREMENT TN0026450 QUANTITY OR LOADING AVERAGE MAXIMUM UNITS ********

- ******** ********
- ********
- ********
- ********
- ******** ********
- ********

03 ******** Req. Mon. MGD DAILYMX ********

- ..........
- ********

04 ******** 2 DEGC DAILYMX MINIMUM ********

110 G RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE I XX I *** NOTE* Read instructions before completin!l this form QUALITY OR CONCENTRATION NO. FREQUENCY EX OF AVERAGE MAXIMUM UNITS ANALYSIS

04 ******** REPORT DEGC CON TIN DAILYMX uous ******** 04 ******** 30.5 DEGC CONTIN DAILYMX uous ******** 04 ******** 5 DEGC CONTIN DAILYMX uous ********

- ********

"* CONTIN uous 19 0.1 0.1 MGIL Five per MOAVG DAILYMX Week ********.

- *****'***

"* CONTIN uous SAMPLE TYPE CALC TO CALC TO CALCTD RCORDR CALCTD CALCTD /' ---;;;;-;_ I I'A ......... NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law thetthis dowment and all attachments were prepared under my direction or supervision in accordance with e system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Site Vice President information, the information submitted is , to the best of my knowiedga and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false Information, including the possibility of fine end imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE DATE 423 843-7001 12 12 14 I AREA I NUMBER MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres,L...f..Q....BOX2000 NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ TN0026450 MAJOR (SUBR 01) F-FINAL Fonn Approved. OMB No. 2040-0004 _______ _ ____ _ RECYCLED COOLING WATER EFFLUENT A TIN: Brad Love *** NO DISCHARGE I XX I *** NOTE: Read Instructions before completin!l this fonn PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MIN.IMUM AVERAGE MAXIMUM IC25 STATRE 7DAY CHR '*:*******

- ********

CERIODAPHNIA TRP3B 1 0 0 PERMIT ********

-43.2 ********

EFFLUENT GROSS VALUE REQUIREMENT MINIMUM IC25 STATRE 7DAY CHR

- PIMEPHALES TRP6C 1 0 0 PERMIT ********

-*43.2 * ********

EFFLUENT GROSS VALUE REQUIREMENT MINIMUM **--*-*** PERMIT REQUIREMENT PERMIT REQUIREMENT PERMIT REQUIREMENT PERMIT REQUIREMENT PERMIT /r-,t . REQUIREMENT ( _%/J ,.--) NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of Jaw that this document and all attachments were prepared under my direction or* supervision in eccordance with a syatem designed to assure that qualified personn( John T. Carlin property gather and e.valuate the information submitted. Based on my inquiry of the person or persons Who manage the system, or those persons directly responsible for gathering the Site Vice President information, the information submitted is , to the best of my knowiedge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY TIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used 1\:: / ... site Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL TELEPHONE DATE 423 843-7001 12 12 14 I AREA I NUMBER MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ AddreS,lL 2000 _ ------____ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ TN0026450 118 G MAJOR (SUBR 01) F-FINAL Form OMB No. 2040-0004 _______ _ ____ _ PERMIT NUMBER WASTEWATER & STORM WATER EFFLUENT A TIN: Brad Love *** NO DISCHARGE I XX I *** NOTE: Read instructions before complelin!l this form PARAMETER >< QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)

- 19 00300 1 0 PERMIT ********

-2 ********

MG/l TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK *.. SOLIDS, TOTAL SUSPENDED SAMPLE ********

- 19 00530 1 0 PERMIT ********

-********

100 MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX WEEK SOLIDS, SETTLEABLE

-25 00545 1 0 PERMIT ********

-********

1 MUL ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE ********

03 ** TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ********

ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX BATCH PERMIT REQUIREMENT PERMIT REQUIREMENT PERMIT REQUIREMENT r--NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that !hill document and all attachments were prepared under my W£/ TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Vice PresiCient ' information, the information submitted is , to lha best of my knowiadge and belief, true, accurate, 423 843-7001 12 12 14 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE L including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER MO DAY lYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 S58 130107 800-NPDES CORRESPONDENCE January 7, 2013 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-DISCHARGE MONITORING REPORT (DMR) FOR DECEMBER 2012 Enclosed is the December 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. Also enclosed is one attachment containing information regarding a collection system overflow that occurred during the reporting period. At no time has there been any observed threat to public drinking supplies, to human health, or the environment. If you have any questions or need additional information , please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted . Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information , the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information , including the possibility of fine and imprisonment for knowing violations. Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga , Tennessee 37 402-2013 S.D. Booker, MOB 1 F-WBN B. E. Brickhouse , BR 4A-C J. T. Carlin, OPS 4A-SQN J. A. Cross, POB 2A-SQN T.R. Markum, BR 4A-C D. B. Nida, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 J.W. Proffitt, OPS 4C-SQN A. A. Ray, WT 11 A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1 E-M Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 January 7, 2013 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-DISCHARGE MONITORING REPORT (DMR) FOR DECEMBER 2012 Enclosed is the December 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. Also enclosed is one attachment containing information regarding a collection system overflow that occurred during the reporting period. At no time has there been any observed threat to public drinking

supplies, to human health, or the environment.

If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 TVA Sequoyah.Nuclear Plant NPDES Permit No. TN0026450 Attachment 1 Description of the Event and Determination of Cause On 12/03/2012 at approximately 19:00 EDT a toilet located in the Mechanical Maintenance shop was observed to be overflowing. The shift manager initiated the SQN Spill Plan and dispatched personnel to investigate. An intermittent stream of sewage was observed overflowing out of the toilet and running approximately 20 feet across the mechanical maintenance shop floor before it entered a floor drain. There were no solids observed in the overflow. On 12/03/2012 at approximately 20:00 EDT the sewage overflow was stopped from entering the floor drain. All remaining sewage residue in the mechanical maintenance shop was neutralized and cleaned up. The floor drain flows to the service building sump. The service building sump is automatically pumped into the SQN Yard Drainage System with discharges to the Yard Drainage Pond. The Yard Drainage Pond discharges to the Diffuser Pond, which discharges through Outfall 101 to the Tennessee River. At no time was there any observed threat to the* public drinking

supplies, to human health, or the environment.

The overflow was caused by a mass of foreign material becoming lodged in a check valve in the sewage ejector system. The check valve was not able to fully close causing the toilet to overflow as the level in the sewage system increased. Period of Discharge The overflow was observed at approximately 19:00 EDT on 12/03/2012. The sewage overflow was entering the floor drain as personnel responding to the spill arrived. The sewage was stopped from entering the floor drain on 12/03/2012 at approximately 20:00 EDT. The estimated time of discharge was approximately 1 hour. Steps Being Taken to Reduce, Eliminate, and Prevent Recurrence This incident was entered into the TVA Corrective Action Program. Sequoyah has closed the two restrooms in the mechanical maintenance shop until further review of this incident has been conducted. Additionally, plans are being developed to install physical barriers between the restroom and local floor drains that will aide in preventing future overflows from reaching the floor drains. PERMITTEE NAME/ADDRESS . (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2o40-0004 _______ _ ____ _ DISCHARGE NUMBER DIFFUSER DISCHARGE EFFLUENT ATTN: Brad Love *** NO DISCHARGE D *** NOTE: Read instructions before complelinR this fonn PARAMETER

x QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM LiN ITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ********

27.6 0 31/31 RCORDR -04 CENTIGRADE 00010 1 0 PERMIT ********

-********

Req. Mon. DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS --MODELD TEMPERATURE, WATER DEG. SAMPLE ********

15.4 0 31/31 -04 CENTIGRADE 00010 z 0 PERMIT ********

-********

30.5 DEG.C. .CONTI CALCTD INSTREAM MONITORING REQUIREMENI DAILYMX NUOUS -. **-1--*-o==- TEMP. DIFF. BETWEEN SAMP. & SAMPLE ********

3 0 31/31 CALCTD -04 UPSTRM DEG.C 00016 1 . 1 PERMIT ********

-********

5 DEG.C. CONTI CALC TO EFFLUENT GROSS REQUIREMENT DAILYMX NUOUS FLOW, IN OR THRU SAMPLE ******** 1534 ******** ********

0 31/31 RCORDR 03 -TREATMENT PUNT 50050 1 0 PERMIT ******** Req. Mon. MGD ********

-CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ********

0.020 0.037 0 14/31 GRAB -19 50060 1 0 PERMIT ********

-******** 0.1 0.1 MGIL FIVE PER CALCTD EFFLUENT GROSS* REQUIREMENT MOAVG DAILY MAX WEEK TEMPERATURE -C;RATE OF SAMPLE ******** 1 ********

0 31/31 CALCTD 62 -CHANGE 82234 1 0 PERMIT ******** 2 DEG ********

- - - - -CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILYMX C/HR NUOUS SAMPLE PERMIT REQUIREMENT

/Tr l /' /l NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ""'"' __ jt 1 rr TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified person el John T. Carlin properly gather and evaluate the infonnation submiHed. Based on my inquiry of the person o persons who manage the system. or those persons directly responsible for gathering the i President 423 843-7001 13 01 07 infonnatron. the infonnation submiHed is , to the best of my knowledge and belief. true, accurst .. , Site Vice President and complete I am aware that there are srgnificant penalties for submiHing false infonnat1on, OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. AREA I OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND OF ANY TIONS (Reference all attachments here) No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred:

1. Floguard MS6236 (max. calc. cone. was 0.051 mg/L-Iimit 0.2mg/L)

2. Biodetergent 73551 (max. calc. cone. was 0.051mg/L-Iimit 2.0mg/L)

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 Mean# of Water Mean# of Water NOTES:% Sample Date ZM/m3 %Settlers Temp. ("C) Sample D;ite Asiatic Temp. ("C) LOCATION Gravid Asiatic COLLECTED BY Ciamslm3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW W8E 01/17/2011 0 0 10 01/17/2011 0 10 P8 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 88 02/14/2012 o* 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 W8E 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 W8E 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 W8E 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 W8E 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 W8E 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 P8 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 P8 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May2012 No Samples Collected June 2012 No Samples Collected July 2012 No Samples Collected August 2012 No Samples Collected September 2012 No Samples Collected 10/23/2012 394 8 17 10/23/2012 82 17 1-ISV-24-1234 WAW 10/30/2012 34 50 17 10/30/2012 17 17 1-ISV-24-1234 WAW 11/06/2012 0 0 17 11/06/2012 0 17 1-ISV-24-1234 WAW 11/13/2Q12 0 0 15 11/13/2012 0 15 1-ISV-24-1234 WAW 11/20/2012 0 0 13 11/20/2012 0 13 1-ISV-24-1234 WAW. 11/28/2012 0 0 12 11/28/2012 0 12 1-ISV-24-1234 WAW-12/04/2012 0 0 12 12/04/2012 0 12 1-ISV-24-1234 WAW 12/12/2012 0 0 11 12/12/2012 0 11 1-ISV-24-1234 WAW 12/18/2012 0 0 24.6 12/18/2012 o* 24.6 1-25-545 CR PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres.l!.__.f.O. BOX2000 _______ _ ____ _ AlTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 PERMIT NUMBER MAJOR (SUBR 01) F-FINAL Form Approved. OMB lllo. 2040-0004 BIOMONITORING FOR OUTFALL 101 EFFLUENT

- - NO D.ISCHARGE D *** NOTE: Read instructions before complelinQthis form PARAMETER

>< QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE AVERAGE MAXIMUM UNITS MINIMUM . AVERAGE MAXIMUM* IC25 STATRE 7DAV CHR SAMPLE ********

Monitoring

-CERIODAPHNIA Not Required TRP3B 1 0 PERMIT ********

-43.2 ********

EFFLUENT GROSS REQUIREMENT MINIMUM -*---***** -* IC25 STATRE 7DAV CHR SAMPLE ********

Monitoring

-PIMEPHALES Not Required TRP6C 1 0 PERMIT ********

-43.2 ********

EFFLUENT GROSS REQUIREMENT MIMINUM ----------

SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT

/ 1-1. Lf\ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER '""""""'W-**o.w*****- .... -_,..,....._:t JA\j direction or supervision in accordance with a system designed to assure that qualified person el John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who managa the system. or those persons directly responsible for gathering the information, the information submitted is

to the best of my knowledge and belief, true. accurate, Site Vice President and complete.

I am '!WOre that there. are significant penalties for submitting false information, Including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in December 2012. EPA Form 3320-1 (REV 3199) Previous editions may be used !! vrce Pre 1dent OF PRINCIPAL EXECUTIVE OF R OR AUTHORIZED AGENT EX OF TYPE UNITS ANALYSIS 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL --*--TELEPHONE DATE 423 843-7001 13 01 07 I AREA I NUMBER MO DAY CODE Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres!,_ _f.Q._BOX 2000 ___________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) 103 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 ____ _ LOW VOL. WASTE TREATMENT POND EFFLUENT A TIN: Brad Love *** NO DISCHARGE D *** NOTE* Read instructions before completinR this form PARAMETER >< QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINiMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ********

7 ******** 8 0 13/31 GRAB

- 12 00400 1 0 PERMIT ********
- 6 ********

9 su THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK -*----**- GRAB--SOLIDS, TOTAL SUSPENDED SAMPLE ********

12 13 0 2/31 ** 19 00530 1 0 PERMIT ********

- ********

30 100 MG/l TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH ***--* -GRAB OIL AND GREASE SAMPLE ********

<4 <6 0 2/31 ** 19 00556 1 0 PERMIT ********

- ********

15 20 MG/l TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 0.963 1.205 ********

0 31/31 RCORDR 03 ** TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD ********

.. SEE RCORDR EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX PERMIT SAMPLE PERMIT REQUIRJ:MENT SAMPLE PERMIT REQUIREMENT

-*--.. -SAMPLE PERMIT REQUIREMENT

/ !?fy /) NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of Jaw that this document and all attachments wara prepared under my \£ TELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the \: _,;}He '\1 ce President Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 13 01 07 and complete. I am aware that there are significant penalties for submitting false information, SIGNA'TORE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER MO DAY TYPED OR f'RINTED CODE COMMENTS AND OF ANY TIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres.!!..._ _E.Q....BOX 2000 ___________ _ SODDY

DAISY JN 37384

=

ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 PERMIT NUMBER MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE I XX I -* Fonn Approved.

OMB No. 2040-0004 NOTE: Read instructions before complelin!l this tonn PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY

sAMPLE AVERAGE MAXIMUM UNITS MINIMUM TEMPERATURE, WATER DEG. SAMPLE ********

-CENTIGRADE 00010 1 0 PERMIT ********

-******** EFFLUENT GROSS VALUE REQUIREMENT --TEMPERATURE, WATER DEG. SAMPLE ****'****

-CENTIGRADE 00010 z 0 PERMIT ********

-******** INSTREAM MONITORING REQUIREMENT SAMPLE----****-* .... ;;;;---TEMP. DIFF. BETWEEN SAMP. & ********

-UPSTRM DEG.C 00016 1 0 PERMIT ********

-******** EFFLUENT GROSS VALUE -REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE ********

03 PUNT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** EFFLUENT GROSS VALUE REQUIREMENT DAILYMX CHLORINE, TOTAL RESIDUAL SAMPLE ********

- 50060 1 0 PERMIT ******** ........ -****"*** EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE -C, RATE OF SAMPLE ********

04 CHANGE 82234 1 0 PERMIT ******** 2 DEGC ******** EFFLUENT GROSS VALUE REQUIREMENT DAILYMX SAMPLE PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin 1 CertifY under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel AVERAGE MAXIMUM ********

REPORT DAlLYMX ********

30.5 DAILYMX ----********

5 DAILYMX ********

0.1 0.1 MOAVG DAILYMX ********

- - - - ******** ********

/"\ properly gather and evaluate the information submitted. Based on my inquiry of the person or 'Y l persons who manage the system, or those persons directly responsible for gathering the "-i--I.J.--181W\Ifl::8 resident information, the informalion submitted is , to the best of my knowledge and belief, true, accurate, Site Vice President and complete. I am aware that there are significant penalties for submitting false information, l-----=-==-==-=-====--------1including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Fonn 3320-1 (REV 3/99) Previous editions may be used EX OF TYPE UNITS ANALYSIS 04 DEGC CONTIN CALC TO uous --04 DEGC CONTIN CALC TO uous 04 DEGC CONTIN CALC TO uous -** CONTIN RCORDR uous 19 MG/L Five per CALCTD Week -.. CONTIN CALCTD uous --TELEPHONE DATE 423 843-7001 13 01 07 AREA NUMBER MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} DISCHARGE MONITORING REPORT (DMR} MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Addres,L ...f..Q,_BOX 2000 ___________ _ TN0026450 PERMIT NUMBER ____ _ A TIN: Brad Love PARAMETER X QUANTITY OR LOADING AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ********

- CERIODAPHNIA TRP3B 1 0 0 PERMIT ********

-MINIMUM 43.2 RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE I XX I *** NOTE* Read instructions before completin!l this form QUALITY OR CONCENTRATION NO. FREQUENCY EX OF AVERAGE MAXIMUM UNITS ANALYSIS

23 ********

PERCENT SEMI SAMPLE TYPE COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL ----IC25 STATRE 7DAY CHR SAMPLE ********

- ********

PIMEPHALES TRP6C 1 0 0 PERMIT ********

-43.2 ********

EFFLUENT GROSS VALUE REQUIREMENT MINIMUM -*--SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE

PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT

--SAMPLE PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Site Vice President information,.the information submitted is , to the best of my knowiedge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false Information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY TIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used ,., .. ./ resident EXECUTIVE OFFICER OR AUTHORIZED AGENT 23 PERCENT SEMI COMPOS ANNUAL --* --**-TELEPHONE

DATE 423 843-7001 13 01 07 I AREA I NUMBER MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA-SEQUOYAH NUCLEAR PLANT Address P.O. BOX2000 ============ _______ _ ____ _ A TIN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ 118 G DISCHARGE NUMBER MAJOR (SUBR 01) F-FINAL WASTEWATER & STORM WATER EFFLUENT

- - NO DISCHARGE I XX I *** Form Approved.

OMS No. 2040-0004 NOTE* Read instructions before completin!l this form PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE AVERAGE MAXIMUM UNITS MINIMUM OXYGEN, DISSOLVED (DO) SAMPLE

-00300 1 0 PERMIT ********

-2 EFFLUENT GROSS REQUIREMENT MINIMUM -*--SOLIDS, TOTAL SUSPENDED SAMPLE ********

- 00530 1 0 PERMIT ********

-******** EFFLUENT GROSS REQUIREMENT ---*----*--- SOLIDS, SETTLEABLE SAMPLE ********

- 00545 1 0 PERMIT ********

-******** EFFLUENT GROSS REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE ******** 03 TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ******** EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT SAMPLE PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin I Certify under penalty of Jaw that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the informaiion submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the AVERAGE MAXIMUM ********

- - - - ******** ********

100 DAILYMX *---********

1 DAILYMX ********

Site Vice President information, the information submiHed is , to the best of my knowledge and belief, true, accurate, end complete. I am awere that there are significant penalties for submitting false information, 1-------=-:==-==-=--=----::-:-:==--------lincluding the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Previous editions niay be used EX OF TYPE UNITS ANALYSIS 19 MG/l TWICE/ GRAB WEEK 19 MG/l TWICE/ GRAB WEEK f-*-----25 ML/l ONCE/ GRAB MONTH -* ONCE/ ESTIMA BATCH TELEPHONE DATE 423 843-7001 13 01 07 AREA NUMBER MO DAY CODE Page 1 of 1 REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-December 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 1/04/2013 CONCURRENCES Name R c Signature -Comment Date v N B.M. Love X L2._ (" ') \, \ Y'Z'3 iJ;v l[ :l L.M. Koby X "--J Ill 'I 4 /e'J J X J.A. Cross* X . t' /1 fi I '--I INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended

purpose, logical in construction, and clear in meaning in the eyes of the recipient.

A concurrence signature indicates that the individual would be willing to* sign the document for the agency.}}

ML13289A202: Difference between revisions

Revision as of 22:10, 3 July 2018

Contents

Text

Dear Ms. Morgan:

Dear Ms. Morgan:

Dear Ms. Morgan:

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Dear Ms. Morgan:

Dear Ms. Morgan:

==

13%. Upper PMSD bound determined by USEPA (90th percentile)

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12%. Upper PMSD bound determined by USEPA (90th percentile)

Dear Ms. Morgan:

Dear Ms. Morgan:

Dear Ms. Morgan:

Dear Ms. Morgan:

Dear Ms. Morgan:

Dear Ms. Morgan:

=

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ML13289A202: Difference between revisions

Revision as of 22:10, 3 July 2018

Text

Dear Ms. Morgan:

Dear Ms. Morgan:

Dear Ms. Morgan:

Dear Ms. Morgan:

=

=

Dear Ms. Morgan:

Dear Ms. Morgan:

==

13%. Upper PMSD bound determined by USEPA (90th percentile)

\ =' Ll='f=======

og=*=\1=*=\'1=====

12%. Upper PMSD bound determined by USEPA (90th percentile)

Dear Ms. Morgan:

Dear Ms. Morgan:

Dear Ms. Morgan:

Dear Ms. Morgan:

Dear Ms. Morgan:

Dear Ms. Morgan:

=

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