Text

Operating Corporation - Response to Request for Additional Information Regarding the Review of the License Renewal Application
	ML070180367
Person / Time
Site:	Wolf Creek
Issue date:	01/11/2007
From:	Garrett T; Wolf Creek
To:	; Document Control Desk, Office of Nuclear Reactor Regulation
References
	ET 07-0001
	Download: ML070180367 (435)
	v • d • e

W LF CREEK* NUCLEAR OPERATING CORPORATION Terry J. Garrett Vice President, Engineering January 11,2007 ET 07-0001 U. S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, DC 20555

References:

1) Letter ET 06-0038, dated September 28, 2005, from T. J.Garrett, WCNOC, to USNRC 2) Letter WM 06-0046, dated November 17, 2006, from M. W.Sunseri, WCNOC, to USNRC 3) Letter dated December 12, 2006, from V. M. Rodriguez, USNRC to T. J. Garrett, WCNOC Docket 50-482: Wolf Creek Nuclear Operating Corporation's Response to Request for Additional Information Regarding the Review of the License Renewal Application for Wolf Creek Generating Station

Subject:

Gentlemen:

Reference 1 submitted Wolf Creek Nuclear Operating Corporation's (WCNOC) application for renewal of the operating license for the Wolf Creek Generating Station (WCGS). Reference 2 provided supplementary environmental information to support the application.

NRC staff identified in Reference 3, areas where additional information and data analysis is needed to develop the environmental impact statement.

The Attachment to this letter provides WCNOC's response to the request for additional information.

It lists each NRC question followed by WCNOC's response to each of those questions.

A/i P.O. Box 411 / Burlington, KS 66839 / Phone: (620) 364-8831 An Equal Opportunity Employer M/F/HCNET ET 07-0001 Page 2 of 2 Enclosure 1 includes the drawings of the discharge structure.

Enclosure 2 provides tables on riparian/wetland communities.

Enclosure 3 includes microbiological monitoring data.Enclosures 4, 5 and 6 include the most recent chemistry effluent monitoring data, recent fishery monitoring reports and maps of the John Redmond Wildlife Area and the Flint Hills National Wildlife refuge respectively.

Enclosure 7 provides tables of wildlife present in the transmission line corridor.

Enclosure 8 provides a map of Coffey County Zoning Districts.

Enclosure 9 provides a table of land use in the vicinity of Wolf Creek Generating Station and associated transmission lines.There are no commitments associated with this submittal.

If you have any questions concerning this matter, please contact me at (620) 364-4084, or Mr. Kevin Moles at (620) 364-4126.Very truly urs, Terry J. Garrett TJG/rlt Attachment Enclosures cc: J. N. Donohew (NRC), w/a B. S. Mallett (NRC), w/a V. G. Gaddy (NRC), w/a V. M. Rodriguez (NRC), w/a, w/e Senior Resident Inspector (NRC), w/a Attachment to ET 07-0001 Page 1 of 9 Response to Request for Additional Information' Aquatic Ecology Additional information required pursuant to 10 CFR 51.41, 51.45(c), 51.53(c)(3)(ii)(B), 51.53(c)(3)(iii), and 51.70(b).1) Provide drawings and a detailed description of the discharge structure.

WCNOC Response Coffey County Lake Discharge Coffey County Lake (CCL), formerly known as the Wolf Creek Cooling Lake, is formed by one main earth-rolled dam constructed across Wolf Creek and five saddle dams built along the periphery of the lake.A service spillway and an auxiliary spillway are provided on the east abutment of the main dam to pass floods up to and including the probable maximum flood (PMF). The service spillway is an uncontrolled concrete ogee-crested spillway that is semicircular in plan. The crest length is 100 feet and the crest elevation is 1088 feet. This concrete service spillway is approximately 14 feet high, and water discharges through it via a 30-foot concrete chute to a stilling basin. The auxiliary (emergency) spillway is located approximately 1500 feet east of the service spillway and is of the open cut type with a crest length of 500 feet and a crest elevation of 1090.50 feet.(USAR 2.4.8.2.2)

A low-level outlet is located near the west abutment of the main dam. The outlet is provided with a 60-inch diameter outlet pipe. A 30-inch diameter blowdown pipe branches from the outlet pipe. The blowdown pipe is provided with an 18-inch free discharge valve, which has a maximum blowdown discharge capacity of approximately 90 cubic-feet per second (cfs) at lake normal operating level of 1087.0 feet MSL. This blowdown system is designed to blowdown water to regulate the water quality of the cooling lake. The 60-inch diameter pipeline is to be used when necessary to drain the cooling lake to permit inspection and repairs of the main dam. Although the dam has provisions for releasing water to Wolf Creek such releases are infrequently performed.

Enclosure 1 includes drawings and figures of the service and auxiliary spillways, and the cooling lake blowdown discharge structure.

Makeup Water Discharge Makeup water from the Makeup Water Screenhouse (MUSH) located below John Redmond Dam is pumped through a 54-inch diameter pipe and discharges into CCL at the Makeup Water Discharge Structure (MUDS) located on the western shore of the cooling lake. The structure consists of a stilling basin/sump, wherein the Makeup Water Pipeline discharges.

From the sump the Makeup Water flows over a weir and down a spillway to the cooling lake. (FD-WL WC, Rev 6) Two drawings, Figure 2.4-1, General Arrangement and Figure 3.4-7, Makeup Water Discharge Structure, are located in Enclosure

1.

Attachment to ET 07-0001 Page 2 of 9 Circulating Water Discharge The circulating water is pumped from the Circulating Water Screenhouse (CWSH) intake structure bays through a 12 foot-diameter inlet pipe to the steam condenser.

The warmed water then flows from the condenser through a 12 foot-diameter outlet pipe to the circulating water discharge structure (CWDS). This structure has a discharge well which overflows into a 40-foot wide apron and then onto the surface of the Lake.

References:

1. Wolf Creek Updated Safety Analysis Report, Section 2.4.8.2.2, "Spillways", Revision 19.2. FD-WL-02-WC, Rev 6, Cooling Lake Makeup Water and Blowdown System Makeup Water System, System Description.

3. Wolf Creek Generating Station Environmental Report, Operating License Renewal Stage, 2006, Section 3.1.2.2) Provide information on potential riparian/

wetland communities in the project area, including along the transmission line.WCNOC Response This is a review of the potential riparian and wetland communities in the vicinity of Wolf Creek Generating Station (WCGS). Such communities applicable to WCGS include: 1. The riparian areas of Wolf Creek associated with Coffey County Lake (CCL) and the shoreline and shallow water areas of CCL, 2. Shoreline and shallow water areas of John Redmond Reservoir (JRR), 3. Riparian areas of the Neosho River and, 4. Riparian and wetland areas traversed by applicable transmission lines.This review summarizes these natural communities based on past surveys, current literature, and staff biologist observations.

It is not an exhaustive survey or data presentation, rather a general overview.

Because there are no plans for refurbishment or additional construction, riparian or wetland resources will not be impacted to support WCGS license renewal.Enclosure 2 to this letter contains the tables referenced in the following discussion.

Wolf Creek and Coffey County Lake The riparian areas of Wolf Creek upstream and downstream of CCL are typical of the Oak-Hickory forests found in east-central Kansas. They are medium-tall, multilayered, broadleaf deciduous forests on the first and second terraces adjacent to streams (NRC 1975).Quantitative analyses of the lowland woods within the Oak-Hickory forests were conducted during the initial licensing process for WCGS (Kansas Gas and Electric, KGE, 1982). Table 1 Attachment to ET 07-0001 Page 3 of 9 of Enclosure 2 presents a list of the plant species and their relative abundance that were monitored for the lowland woods, which comprise the riparian areas of Wolf Creek.Hackberry (Celtis occidentatlis) was dominant or codomiant within woodlands of Wolf Creek.Common associates were black walnut (Juglans nigra), American elm (Ulmus americana), white bitternut hickory (Carya cordiformis), silver maple (Acer saccharinum), bur oak (Quercus macrocarpa), green ash (Fraxinus pennsylvanica), and Kentucky coffee tree (Gymnocladus dioica). Inspection of tree species distribution of the lowland woods showed that silver maple, American elm, green ash, and sycamore (Platanus occidentalis) were more common within the frequently inundated sites, whereas hackberry, red bud (Cercis canadensis), Kentucky coffee tree, hickories (Carya spp.) and oaks (Quercus spp.) occurred on higher, well-drained sites (KGE 1982).Shrub component species include coralberry (Symphoricarpos orbiculatus), poison ivy (Rhus radicans), wild gooseberry (Ribes missouriense), hackberry, and elms. Ground layer components included spreading chervil (Chaerophyllum procumbens), wood nettle (Laportea canadensis), Virginia wild rye (Elymus virginicus), clearweed (Pilea pumila), and fescue (Festuca obtuse), all typical floodplain species (NRC 1982).The shoreline and shallow water habitat vegetation of CCL are typical of wet soil or periodically flooded habitats.

Observations of the shoreline vegetation include species tolerant of various degrees of inundation or wet soil conditions.

Initially, cottonwood (Populus deltoides), black willow (Salix nigra), and buttonbush (Cephalanthus occidentalis) are common along the shorelines.

In areas more frequently flooded, shallow water plants such as cattails (Typha spp.), smartweeds (Polygonum spp.), and water primrose (Ludwigia peploides) are common.Emergent and submersed plants in shallow, but slightly deeper water include American lotus (Nelumbo lutea), pondweeds (Potemogeton spp., primarily nodosus and foliosus), and Naiad (Najas minor).As CCL water level fluctuates, mudflat areas develop. Colonization of these areas by plants are expected to be similar to that studied at JRR (NRC 1975). Table 1 of Enclosure 2 identifies common species and relative abundance within the mudflat areas of JRR. These species are typical for this region, and can be expected along the CCL mudflats.

Initially, two plant communities will occupy the mudflats.

In poorly drained areas, plants typical of wet marshy areas of JRR will dominate.

These include sedges (Carex spp.), cattails, black willow, and arrowhead (Saggitaria latifolia).

In areas where re-inundation is infrequent (4 to 5 years), some advance seral communities will replace the pioneer communities.

These include flood tolerant woody vegetation, such as black willow, buttonbush, and cottonwood.

Since WCGS operation began in 1985, riparian protection and enhancement activities have been completed.

These include construction of approximately 25 acres of shallow water ephemeral wetlands, protection of old-growth oak-hickory woodland, planting of bottomland woods, establishment of native grasses for buffers along CCL shorelines, areas preserved for natural succession, and livestock exclusion.

John Redmond Reservoir The wetland and shallow coves of JRR are dominated by swamp smartweed, in addition to other smartweed species (Polygonum spp.), bulrush (Scirpus spp.), cattail, spike-rush (Eleocharis spp.), and sedge (Carex spp.). Some stands of silver maple, black willow, and Attachment to ET 07-0001 Page 4 of 9 eastern cottonwood are also present. On the reservoir drawdown areas (mudflats) weedy annuals such as cocklebur (Xanthium strumarium), foxtail grass (Setaria spp.) and barnyard grass (Echinocloa spp.) are common species (U. S. Army Corp of Engineers, USACE, 2002).A species list and relative abundance compiled during mudflat vegetation surveys of JRR (KGE 1982) is presented in Table 1 of Enclosure 2.Neosho River The riparian areas of the Neosho River upstream and downstream of JRR are characterized in USACE (2002). Basically, riparian woodlands are a bottomland hardwood type dominated by American elm, green ash, eastern cottonwood, black willow, black walnut, sycamore (Plantanus occidentalis), silver maple, burr oak, box-elder (Acer negundo), and hackberry.

Downstream from JRR, most of the flood plain vegetation along the Neosho River and its major tributaries can be described as the riparian woodland type. Islands, point bars, and first terraces are dominated by more wet soil tolerant species such as eastern cottonwood, silver maple, and box-elder.

Slightly higher elevation second terraces support eastern cottonwood, green ash, American elm, black walnut, hackberry, and burr oak.Flood plain shrubland (under-story) growing along riparian areas include coralberry, greenbriar, rough-leaf dogwood (Comus drummondi), American plum (Prunus americana), and wild grape (Vitae spp.). Downriver from JRR, these shrublands occupy recently scoured islands, point bars, and riverbanks.

Sandbar willow (Salix interior), rough-leaf dogwood, and buttonbush invade rapidly and eventually are replced by black willow, silver maple, and eastern cottonwood.

Transmission Line Corridors The transmission lines included in this review of riparian areas traversed include the Wolf Creek-Rose Hill 345 kilovolt (kV) line, the portion of the LaCygne -Benton 345 kV transmission line rerouted for WCGS, the WCGS to Sharpe Kansas Electric Power Cooperative (KEPCO) 69 kV line, and the Wolf Creek tap of the Burlington

-Athens 69 kV line.Wolf Creek -Rose Hill Transmission Line: The Wolf Creek -Rose Hill line extends approximately 98 miles from WCGS in a southwesterly direction toward the Rose Hill substation east of Wichita, Kansas. Easements are 150 feet in width, which totals 1691 acres of right-of-way.

General land use classifications traversed by this line include cropland, grazing, woodland, idle land, waterways and roads. This right-of-way traverses a total of 4,950 feet (18.2 acres) of riparian woods, and 480 feet (1.8 acres) of waterways (Table 2 of Enclosure 2). The riparian and waterways traversed by the line represents approximately 1 percent of the total right-of-way.

Major rivers and associated watersheds traversed by the Wolf Creek -Rose Hill transmission line include the Neosho River primarily in Coffey County, the Verdigris and Fall Rivers primarily in Greenwood County, and the Walnut River primarily in Butler County. Riparian areas within these watersheds are substantially similar to that described for the Neosho River above.LaCygne -Benton Transmission Line (rerouted portion):

Attachment to ET 07-0001 Page 5 of 9 The portion of the LaCygne -Benton 345 kV transmission line rerouted around CCL is approximately 7.7 miles. Most of this line was constructed on WCGS lands. Assuming a 150 feet wide corridor, this rerouted line encompasses nearly 140 acres. Based on aerial photographs, land use types, including wetland and riparian habitats are presented in Table 3 of Enclosure

2. There are 12.1 acres of riparian, surface water, shoreline, and wetland acres included in the corridor, or 8.7 percent of the total.WCGS Tap of Burlington

-Athens 69 kV Transmission Line: The WCGS tap of the Burlington

-Athens 69 kV transmission line traverses approximately 4.1 miles, most of which are on WCGS lands. There are no streams or associated riparian areas traversed by this line, however, this line does cross over portions of CCL. Applying a corridor width of 150 feet, surface water, shoreline, and wetland acres included in the corridor total 4.7, or 6.4 percent of the total (Table 4 of Enclosure 2).WCGS to Sharpe KEPCo 69 kV Transmission Line: The WCGS to Sharpe KEPCo 69 kV transmission line is approximately 3 miles, and does not cross any wetland, shoreline, or riparian areas. Right-of-way for this line is primarily parallel to local roads.3) Provide the most recent data obtained from any microbiological monitoring program.WCNOC Response Enclosure 3 includes the WCGS Construction Environmental Monitoring Program Report, February 1981 -January 1982 and the WCGS Operational Phase Environmental Monitoring Program Report, Final.4) ER Section 6.2 notes that routine mitigation and monitoring programs are conducted, including effluent chemistry monitoring and water quality and fishery monitoring of Coffey County Lake. Provide at a minimum the most recent set of these data.WCNOC Response Enclosure 4 includes the most recent chemistry effluent monitoring data. Included is a figure and a map of the NPDES Outfall sampling points. WCGS sample analysis results from November 1, 2006 to November 30, 2006 are included in a table format. Also provided in the Enclosure are analytical results from an accredited environmental laboratory, circulating water bromination schedule, and log entries for discharges through outfall 003A and 003B.Enclosure 5 includes fishery monitoring reports for years 2002, 2003, 2004 and 2005.

Attachment to ET 07-0001 Page 6 of 9 Critical and Important Terrestrial Habitats Additional information required pursuant to 51.53(c)(3)(ii)(E).

1) Provide the locations of the John Redmond Wildlife Area and the Flint Hills National Wildlife Refuge on one or more maps.WCNOC Response Enclosure 6 includes maps of John Redmond Wildlife Area and the Flint Hills National Wildlife Refuge. These maps are also available online at http://www.swt.usace.army.mil/LIBRARY/Webhuntinqmaps/iohnredmond.pdf and http://flinthills.fws.qov (accessed January 5, 2007).2) ER Section 2.4 mentions the following terrestrial species: bald eagle, peregrine falcon, and osprey. Provide a discussion of other wildlife, including mammals, reptiles, invertebrates, and birds (other than eagles) that may be present in the transmission line corridor.WCNOC Response This is a review of potential terrestrial fauna that may be present within the transmission line corridors related to Wolf Creek Generating Station (WCGS). The transmission lines reviewed include the portion of the LaCygne -Benton 345 kV line rerouted around Coffey County Lake (CCL), and the Wolf Creek -Rose Hill 345 kV line. These lines traverse habitat types common to eastern and south-central Kansas, and wildlife species common to these habitats can be expected to occur within the corridors.

Except for invertebrates, potential terrestrial species present are characterized by generic habitat where they may be found.The rerouted LaCygne -Benton line traverses approximately 7.7 miles around the northern reaches of CCL. Habitats traversed include the WCGS site, CCL and shorelines, native tall grass prairie, cropland, grazed rangeland, and hay meadows. The corridor is also adjacent to bottomland woodland and mixed shrub and grass areas.The Wolf Creek -Rose Hill line traverses similar habitats, with much higher proportions of cropland and grazed native rangeland than the LaCygne -Benton line. This line is within Coffey, Greenwood, and Butler counties.

It extends southwestward from WCGS for 98 miles to the Rose Hill Substation.

Terrestrial invertebrate species that may be present in the corridors include primarily arthropods.

These include insects, spiders, mites, ticks, scorpions, daddy long legs, and others of the class Arachnida.

A conservative estimate of the number of named species of this class is 30,000. It is not known exactly how many species of insects occur in the WCGS area, or in Kansas. 15,000 to 20,000 insect species may exist in Kansas (White and Salsbury 2000). A representative checklist of Kansas insects is available online at www.gpnc.org (accessed December 15, 2006). There are no invertebrate species federally listed as threatened or Attachment to ET 07-0001 Page 7 of 9 endangered within any of the counties traversed by the transmission lines. One insect species, the prairie mole cricket (Gryllotalpa major), is listed by the Kansas Department of Wildlife and Parks (KDWP) as a species in need of conservation and present within Coffey County (KDWP 2006).Terrestrial vertebrate wildlife includes amphibians, reptiles, birds and mammals. Table 1 of Enclosure 7 summarizes representative amphibian and reptile species that may occur within the Coffey, Greenwood, and Butler Counties, Kansas, which includes WCGS and the applicable transmission line corridor.

This summary is not an exhaustive species list, and some species may well be found in multiple habitat types. References used for this table were from Collins (1993).Potential bird species within the three counties are numerous due to their mobility, and migratory nature, and the diversity of habitats present. Currently up to 465 bird species have been documented in Kansas (Kansas Ornithological Society, KOS, 2003). Table 2 of Enclosure 7 lists common species that may be expected to occur within the vicinity of WCGS and applicable transmission line corridor.

References used to compile this list include WCGS (1984), Thompson and Ely (1989 and 1992), and KOS (2003).Potential mammal species within the three counties are included in Table 3 of Enclosure 7.Species account records were obtained in Bee et al, (1981), and Timm et al (2006).Transportation of spent fuel Additional information required pursuant to 10 CFR 51.45(c), 51.70(b), and 51.41 Subpart A, Appendix B, Table B-I.1) Provide information to support the applicability of Table B-1 for transportation of spent fuel, specifically provide the maximum fuel enrichment level and the peak rod average burnup level at WCGS.WCNOC Response The maximum nominal U-235 enrichment level is 5.0 weight percent. The maximum lead rod burnup level is 60,000 MWD/MTU. However, the NRC recently issued a letter [ML061420458]

dated May 25, 2006 from J.D. Peralta (NRC) to B.F. Mauer (Westinghouse) approving an increase in burnup limit to 62,000 MWD/MTU provided PAD 4.0 fuel rod design methodology is used. The fuel rod design analysis for Wolf Creek is done with PAD 4.0.Land Use/Zoning Additional information required pursuant to 10 CFR 51.45(d).1) Provide information on "the status of compliance with applicable zoning and land-use regulations" imposed by State, regional, or local agencies having responsibility for environmental protection.

2) Identify how the site and areas immediately surrounding the site are zoned, the requirements associated with the zoning, and any other land use regulations applicable to the site or surrounding area.

Attachment to ET 07-0001 Page 8 of 9 WCNOC Response The WCGS site is located in the A-1 Agricultural Zone. The Agricultural district is established to encourage the compact development of the urban areas, to preserve productive farm and ranch land and to permit limited nonagricultural uses and low-density dwellings, which would not be incompatible to the rural area and require minimum public services.

Special uses for the Agricultural district include power plants, both conventional and nuclear fueled, for commercial production and sale of energy.The nearby communities of New Strawn and Burlington are in the A-2 Agricultural Transition Zone. The Agricultural Transition district is established to retrain certain rural characteristics, but to also serve as a transition area to accommodate many of the nonagricultural uses normally located in a rural area while anticipating an increasing amount of urbanization including low-density dwellings.

More public services would be anticipated than in the A-1 Agricultural District.Enclosure 8 provides a map of the Coffey County Zoning Districts.

Reference:

Coffey County. 2000. Zoning Regulations of Coffey County, Kansas.Offsite Land Use Additional information required pursuant to 10 CFR 51.45(b).1) Identify areas dedicated to each land-use category in the vicinity of the plant (data, in acres, for Coffey and Lyon Counties would be appropriate) and along transmission lines.WCNOC Response Land use in the vicinity of Wolf Creek Generating Station (WCGS) has been identified using county specific data from the U. S. Department of Agriculture (USDA), National Agricultural Statistics Service (USDA 2002). Land use summaries for Coffey and Lyon Counties are presented as requested.

Land use summaries of Greenwood and Butler Counties are also provided because these are traversed by the Wolf Creek -Rose Hill 345 kilovolt transmission line associated with WCGS. Table 1 of Enclosure 9 summarizes land use in these counties.Visual Aesthetics Additional information required pursuant to 10 CFR 51.45(b).1) Describe external appearance of plant buildings and facilities, including height of structures.

WCNOC Response The principal building complex at the Wolf Creek Generating Station (WCGS) is a group of interconnected buildings oriented in a generally north-south direction.

This complex comprises the central structures and forms the visual foundation for the power block. The main vertical element in the composition of the power block is the domed reactor containment building.

The Attachment to ET 07-0001 Page 9 of 9 structure is approximately two hundred and thirty four foot high. Interconnecting structures include the fuel building, control building, auxiliary building, diesel generator building, and the turbine building.The turbine building is a horizontal structure with a lower profile than the reactor building.

Its steel structure has metal siding and is approximately one hundred and fifty foot high.The radwaste building is located nearby, facing the fuel building.

Also included among the power block structures are the condensate storage tank, refueling water storage tank, reactor makeup water storage tank, demineralized water tank, emergency fuel oil storage tanks, and several transformers vaults.The upper part portion of the reactor and turbine buildings are visible from U.S. Highway 75, which is 2.75 miles west. The station is also visible from a number of local roads, some of which pass within 1.5 miles. Most other structures described are low-visibility structures that do not appreciably change the skyline of the station.Railroad sidings are installed to serve the fuel and turbine buildings.

The main access railroad leads into the site from the north and branches into several spurs, which provide access to the outlying structures and encircle the principal building complex.The major non-power block structures include the administrative building, general office building, Technical Support Center, switchyard, shop building, security building, water treatment plant, warehouse, circulating water pumphouse, circulating water discharge structure, meteorological tower, and the essential service water pumphouse.

Also located around the site complex are several storage tanks and small buildings for storage of acid, compressed gasses, water and fuel oil.The Emergency Operations Facility, Simulator, Visitors Center complex is located about 2.75 miles northwest of the station.In summary, WCGS plant arrangement and structural design is coordinated to establish continuity and to provide both a balance and symmetry of design and a pleasing appearance.

The various site components such as structures, equipment, parking, and railroad spurs are organized in a neat, functional manner with a minimum of visual clutter. To provide variety of texture and color, variegated siding is used on a number of the buildings.

Landscaping is used, where possible, to complement plant appearance.

The plant facilities and grounds are visually pleasing and compatible with the surrounding environment.

Enclosure 1 to ET 07-0001 Drawings and Figures S-0028, Rev. 06, Main Dam STA. 90+00 to STA. 122+58.63 Plan & Profile S-0026, Rev. 11, Main Dam STA. 27+00 to STA. 57+00 Plan & Profile S-0057, Rev. F, Service and Auxiliary Spillways Plan S-0067, Rev. E, Approach & Discharge Channels for Low Level Outlet &Blowdown Structures S-0465, Rev. D, Low Level Outlet & Blowdown Structures Sections & Details Sheet 1 USAR Figure 2.4-1, Rev 0, General Arrangement Environmental Report (Operating License Stage) Figure 3.4-3, Outlet Works Environmental Report (Operating License Stage) Figure 3.4-7, Makeup Water Discharge Structure 8 7 6 5 4 3 2 8 7 6 5 4 I 2~ .150.11 06.as-.LiA ii7 PLAN 220_4 v (\ 7/~/~//I~a --I "aM- a-baa I ----I mar. n a.--n.me -it -.bat a in.I...r. -~-(I.9~~ ~ -4~.h*'--'Iii-H G F~ 4 DI A 0-0 0-e 084 w ...Y1.4ri~~~~

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WOLF CREEK GENERATING STATION UNIT NO. I 1 ENVIRONMENTAL REPORT OPERATING LICENSE STA FIGURE 3.4-3 OUTLET WORKS PL.AiJ MAKE Up '-r= Q'4M ed,, , czj, uay, Ing 1 ... .0,2 a.._ r4z C-FCTl0Q~

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WOLF CREEK GENERATING STATION UNIT NO. I ENVIRONMENTAL REPORT PERNrING LICENSE STAE)FIGURE 3.4-7 MAKEUP WATER DISCHARGE STRUCTURE Enclosure 2 to ET 07-0001 Riparian/Wetland Community Tables Table 1, Plant species within the riparian (lowland woods) of Wolf Creek upstream and downstream of Coffey County Lake (from KGE 1982, Table 2.2-1). Wet and dry mudflat species from vegetation monitoring sites at John Redmond Reservoir.

Table 2, Land uses within the Wolf Creek -Rose Hill transmission line right-of-way (from KGE 1975, Table 3.9-1).Table 3, Land uses within the rerouted right-of-way section of the LaCygne -Benton 345 kV transmission line in the vicinity of Wolf Creek Generating Station.Table 4, Land uses within the right-of-way of the WCGS tap of the Burlington

-Athens 69kV transmission line.Cited Literature TABLE 2.2-1 CD PHYLOGENTIC LISTING OF PLANT SPECIES SAMPLED NEAR WCGS, 1973-78 b.$p,(? a a~~e n4 t.' ,b 0* ~ 5~-e, *-.-~4..b, Community Type: 11. fi' -F', Scientific Name Common Name Irndex Of Relative Community Abundancea Cuni ferae Cupresaaceae Cypress Family Juniperus vhglnlaam L. Fastern redcedar Greel n3les Gr-mi neae Grass Family aromus teotorum L. Down.y chess I A-D I Fronua T-w-T Leyas. Smooth bromv I A-D F --n cusa Thunb. Japanese brome C A-D A1-D_______ -e aflor I.. Meadow fescue I e-stuca OBtusa BiMahler Fescue C reatuca par-raloxa Dvsv. Fescue A-0 os TrS90 E. Canada bluegrass I I I rpali.M. L. Klentucky bluegrass C 1-C I g s piloss (f.. ) Be-.v. Love grass Ip Iabllis (Purah) Steud. Purple lovegrass I I(L.) Smyth. Purple top I I F. I us c-aenis L. Canada wild rye I n_'ýt Tii L. Virginia wild rye U C ieum Nutt. Little barley I C i-C-T-l-a crisreta (L.) Pets. Prairie junegrasi I E grl it T! IWalt.) BSi, Red top I cinna aru ndincea 1, Wood reed-u-ph1nS-er i asp. I. Muhly !Ru--b[e'n Ii--a sa hreberi Gmel. Nimble will I C S"tolu aspe- MTrC-xlh.)

Kunth. Tall dropseed 1 I K toou ne jT tcus Nar h. Dtopseed I brooiaFetc"rolep is Gray Prairie dropseed I Ii~atid ihhlg antha Mrche. 7tree-awn I t-oc--rlToa mis Lam.1) seauv. Leptochloa I C I.eo-cTl oa Tlaslo"ari (Laom.) Gray Laptochloa C-iT cur a (Mich-.) Torr. Side-oars grams P-iaris Wait. maygrada I t'Eit. i r rund -acca L. Rc.ed canary grass Lenersig'viTrg`TnTca--l9lld.

White grass I Dirsi ian2 alls n L.) ScOpu Crab grass I at;pa--m, MichS. Paspal um C I-C i.ni p-. U. Panic grass C C C Pa-nc tas ca ilIIare L. Witch grass;'NiZ-m ac loram Aic It. Pall panic grass D A-0 Ps-lcum &~acTcl tch Chabo Panic grass I;U0- c- -U CD 0a-,~CD cD-) 0 32 C) D CD 0 :E-. CD)CD 0 C CD 3 ~0 C 0 =.CD 0 (0~'CD 0 -=3 CD 0. 0 G)0 TABLE 2.2-1 (Sheet 2)CD 0 CD 0-Community Type: // b'.01 1 " -t .7 Scientific Name Common Name Index of Relative Community Abundance Liliaceae (continued)

Smilacina racemose IL..) Deaf. raise Solomon's seal I I-C----b-itorifl-a-um (walt.) Ell. Solomon's seat l smilax hipida-- -M .Greenbrler A i8[folia L. Creenbrler I Salicales Salicaceac Willow Family oý!ulusdeltotdes Marsh. Cottonwood I A-nra L. Black willow I Juglanlales Juglandaceae Walnut Family Juglans ni ra 11. Black walnut A Carya coTd rmis (Wang.) K. Koch. Bitternut hickory A lj7ry aciniosa (Michx. f.) Loud Shellbark hickory C Fagales Fa g aceae Beech Family Ouercus macrocarpa Michx. Bur oak 0 Quercus N reali Mich.. f. Red oak VQue-*' r rs Muenchh. Pin oak Quercus shsuardtl Buckt, Shumard's oak A Urticales Ulmaceae Elm Family Ulmus sp. L Elm A Ulmus americana L. American elm A ulmus -t Zr Muhi. Slippery elm C L. Flackberry n Moraceae Mulberry Family Maclura pofera (Raf.) Schneld. Osage-oranqT

___us r-r" ga Red mulberry C Urticaceae Nettle Family Urtica dioica L. Stinginv]

nettle C saporteacanadennis (L.) Wedd. Wood-nettle 0 Pilea Gray Clearweed Pa-Fetaria pertnmvanica Muhl. PelLitory C hoehmaa cylinrica (I..) Sw. False nettle I

--A TABLE 2.2-1 (Sheet 3) a a'CC*0.'M Community Type: 64 4, -,~ 4ý &~ -e '4-1, Scientific Name Common N4am. Index of Relative Community Abundance Pu1y 9 Onales poiygonaceae Sinartbeecd Family Runex ACetoseci a L. Red sorrel t RU ex cr9ka1 Curly dockI~1nnsp. L. ol yonum I'i~a iat if.li.. 1'. Smartweed AI Polygonuba L PennsylvaniA smartweed fPjyT punc aturnIEll.

Dotted amartweed I Speraicaria L. Lady, h.umb C C~ T,-jq-rn-T-a

i. Smartveed C.Zygnn nd m False buckwheatI Caryophylalabs Chenopodfaceae Goosefoot Family Chem nodw 1 y M 5S.. Ooosefoot I Chem n d ium album L. Lamb's 'juarterB I CEi R~Ti YbFrsdt L. Plapleleat goaaefoot I~Ii~n~iii refohl I m Aelleon Goosefoot A.1iranthaceae Amaranth Family Amaranthus op. L. Amaranth C Xsareothug 6 attisti l (Nlutt.) Wood ia ter hemp, 1-C Asa-ranthgus-rerIeIu4T L. RedrootI Aizoaceaq Carfetuxed family Mollago verticillata CarpetucedI Portulac Ceat, Purslane Family Portulaca oleracea L, Purolane_1yt 251vrqica t- Springj beauty I Caryophyllaceae Pink Family Stellaria sp. L. Chickweed Ge-ras-iuz vulgatum i.. fouse-ear chickweed IA C nTW-ne ant ITh tMan L. sleepy catchily Mle-ne stella~ta (L.) Alt. f. Starry catapion Ranales Ranunculaccee Crowfoot Pamily Ranunculus ap. L. Buttercup R-an-G-c-ulu-q abortivue L. Small-flowersd crowfoot C TABLE 2.2-1 (Sheet 4)-1 CD 0 CD_CD b &0 "4'b b~" Community Type-Scientific Name Common Name~4~t?Ranonculaceae icontinued))LeIphinitim virescenn Nutt.Tha ITEc.f up. E.T1Tlitrum R1I-A ul Menie lrM t0&aea M enisperoucm canadense L..pap., 'aIe Cruc i terae Lepidium mp. L.D1ýEFE aveiiafTL-O. )Fern.XlBsSiT, lFern.1 GI.AkrabiaS ~anademiS L..koipa sandaIca (Oader) Borbas Sax if ragJaceae Ribes minsouriense Nutt.P I afWanac eaie-Plataitus occidentalis L.Rusaceae Frageia virginianj, Ouchesne U-=vernuc, iRaf.) T'. A Gl.Ce-im Eiand3-sse Jacc 1.ft~~s llghejeis Porter Moa arlina, II FProu s~rOtina FEhrh.p.L.1 imosaceac Des ~nthus illirnoensis Willd.CaesalpinlaCeae Cercia canade nsis L.Cleits-tatriact hos L.~xc~iWuiao~ca L.) .Koch Prairie larkspur Meadow rue Meadow rue Clematis Moonseed Family Canada moonseed Mustard Family Pepperqrass Pepperg rass Drab: Rock creBs Sickle-pod Marsh cress Saxsfrage Family Missouri gooseberry Plane-Tree Family Sycamore Rose Pamily Virginia strawberry Aveng Spring avens Whlte avens Commoti blackberry Agrimony Carolina rose Cherry Slack cherty Hawthorn mimosa lsamily Illinois bundleflover Redbud Honey-locust Kentucky cotfee-trea Index of Relative Comunity Abundance I I I I I l I-C A C A-b I C C I!l 1 1 I A C C TABLE 2.2-1 (Sheet 5)0 CD Community Tye ;F + ~ ~Scientific Name Common Name Index of Relative Community Abundance rabaceae Bean Familyucophaea mutt. Wild indigo I I;; Sp. L. Clover I rifolim rens L. White clover C cTTT-tu T nab i IL.) Deer, Yelll eweetclover I Se-cul Frsh. Prairie turnip I iea-i -teinulreora Purah. Scurfp[a I Petalotemuz op. Hichs. Prairie-clover

]PetaiTotiemm candidum (Willd.) Michx. White prairie-clover a Mixim. Korean lespedeza C 0 rp hoyIes lesperma (T. & G.) Piper Wild beanI f1r M 0 --L.j ElI. Wild bean 1_eemed___um sp. Do. Tick-trefoil Gera..iales Ov al idaceac Wood-sorrel Family Oxalis up. L. Wood-norrel Ovalle stricta L. Yellow wood-sorrel A v als Vior-acea IL. Violet -nod-sorrei C-A Ceraniaceau Geranium Family Geranium caroliniamn L. Crane's-bill I Buphorbiaeceae Spurge Pamily Croton capit-tu-Miche. Croton I Aca yphTo .g.iina Gray Three-seeded m.ercury C C I-C a eroee HAK Spurge I Ei"phorba Eresill Guas Noddt ng spurge U or n1! -ypts eerpf E.nralmei.

SpurgeU-rhl macuslat a Wart.weed 2 oii"h F=T ta Engelm. Spurge C 10!0-Imai co ro Ra Ei L. Flowering epurge C het"r op- i L. Pire-on-the-mountain I marginat e r ursh. Snow-orn-the-mountain Sapi ndales Anavardiaceae Cashe. Family Rhus radica s L. Poison ivy A !=Rhu .. ra Smooth sansac C-A Celdstraeace Staff-tree Pamily g atropurpuroue Jacq. iiahoO C TABLE 2.2-1 (Sheet 6)-D 0-CD ,Q;CD b 4j 4 S bien4fi' 1, oRbun 41 Community

¶~pe: V 04 4' 4Fb Scientific Name Common Name Index Of Relative Community Abundance 4 Aceraccao ACer saccharinum L..ce -nuro I..llippocastanaceae emaculu glabra Willd.Salsam inaceae Iapatiens biflora Walt, hamnales Vitaceae Vitis op. L.Viti8 aestivalis Michx.earthnocusus (L.) Planch.MalvaIes Malvaceae Sida Spinon.o L.S t--o-rat Medic, Parietoles Flyper icaceae!!Xetricum punctatum Lam.Viotaceae Viola sp. L.Viola papililonacoa Pursh.7toTa atl jida U .Don..eriocrpa Schw.Cdact a. es Cactaceam Opuntia compressa (Salisb.)

Macbr.Myrtales Ona'jraceae Oenothera biennis L.iýiaeah-annsuC E.lcircaea aijaisuicata 11laxml.)

piuanch j. Sav.Maple Family Silver maple Boxelder Hors*-Cheotnut Family OhtiO buckeye Touch-me-Not Family Jewelveed Grape Family Grape Summer grape Virginia creeper Mallow Family Sida Plower of an hour Velvetteaf D I C A I C I I-C St. John's wort Family Violet Family Violet Common blue violet A Prairie violet Smooth yellow violet A Cactus Family Prickly pear Evenirn Primrose Family Common evening primrose Biennial gaura Enchanter's nightshade I

TABLE 2.2-1 (Sheet 7)CD 0 Cr CD Commaunity T~ype; 4, 4 4 Scientific Name Common Name Index of Relative Community Abundance umbellae Umbaltlierae Parsley Famlly Sanicula qrgaria Sickn. black snakeroot D T (M.) DC. Ionewort I isFFhiasp.

Ra- .Sweet cicely I L~ua procr n9bee (L.) Crantz Spreading chervil 0 Pastinaca aativa L. Wild parsnip rY2 y-iurm yccolium Mich. RattLesnake-master

!Cornacoae OoDgwood Family Cornus sp. L. Doq9ood I uenaKea Ebenaceae Ebony Pamily EL2!Eyros virginiana L. Persimon I Prioulalea Primulaceae Primrose Family Lysiamchia quadrifolia L. Whorled loosestrife I Gentianalea Oleaceae Olive Pamily Fraxlnus pennoylvanica Marsh. Green ach A Apocynaceae Iobane Pamily APocynn s3p. L. Dogbane K Apocynu cannabinum L,. Indian hemp ! I I-C Asclopiadaceae Milkweed Family j jrl ,.. Common milkweed I ARc asvr .idia Walt. Spider milkweed I Au a"- vantii Engelm. Sullivant's milkweed 1ITTivarre gata L. White milkweedK Polcmoniales Convolvulaceac Morning-glory Family U lacunoaa LC. Morning-glory Convlul-u9sp.

L. Bindweed ConvolvuluS fepium L. Hedge I I-C Polemoniaceae Phlox Pamily Phlox divaricata L. Phlox C Table 2.2-1 (Sheet 8)Cr, 0 (1, 0O Community Type: Scientific Name Hydrophyl I aceae Ellisia nyctelea L.Borag inaceae!in sp. L.yosoti8 Verna Nutt.Verbenaceae Verbena sp. L.Vetbena canadensmi (I..) Britt.Labiatae Prumella vulgaris L.bcutellarL4 ErLa Mlchx.__i_ u mL.Salvia reflexa Norne..--ctiepDt lex (Walt.) Sap, jLMy a uameri c6nu--- Uh Ih.lenulIon-.

Wilid.Sol anaceac S. aro. carolinense L.Phrymaceaeleptostachya L.Scrophulariacae Penstemon tubaeflorus Nutt.v -ron- -11f-1=0 L.VeronUic 71rerLn1 VUThic a EPTI c i is L.Acanthaceae PBuellia strepens L.'la ntaq ina I es Plantaqinace~c at ir inica L.j~nta rug ~T Decne.plantag lanceolata i,.Pla ago stat Michv.CojMon Name Waterleaf Family Nycteles Borage Family Forget-me-not Forget-ie-not Vervain family Vervaln Large-flowered verbena Mint Family Self-heal Skullcap White dead nettle Sage Pitcher's sage Mountain mint American bugle-weed Hedge nettle Nightshade family Ground-cherry Itorse-rnettle Lopneed Family Lopseed Figwort Family Penstemon Speedwell Speed-ell Speedwell Acanthus Family Ruellia Indea of Relative Community Abundalnce C C C-A I C Plantain Family White dwar( plantain C C Rugel's plantain Comemon plantain English plantain Buckhorn plantain TABLE 2.2-1 (Sheet 9)CD b A : Community Type: k, Scientific Name Common Name Indeg of Relative Community Abundance Rubi aLs Rubiaceae "adder Family GaLLm aparina L. Cleavers D Caprifoliaceac Honeysuckle Family Sambucus canadensis L. Common elder I mphoP-crcE-p-oe urbiculatus Moench Coralberry 0 C C-I)Cucurbi tales Cucurh I taces Gourd Family Sicyo angvlata L. Rur-cuc<tber I Cammaulales Campanusacoae Rarebell Family Cam anul americana L. Tall bellflower I-C Troan dt (L.) Nieuvl. Venus' Looking glass 1 TVIada nle I r Imutt.) NieCvl. Venus, looking glass LObel laceae Lobelia Family Lobelia spicatata Lam. bobe L ia Asterales Composite Family Compositac Hellanthus annuus L. Sunflower C-i rl-eT --E-lu-s e o ara Nuit. Common sunflower I Ifel-ianthus lest [ [lrus Pero. Sunflower I-C Helanthus maximiliani Schrader Maximilian sunflower I Velrbealba aternTroFTa (L-) Britt. Winratem A)Nutt.) Woot 4 Standle. Prairie coneflower sldtns Blake Beggar-ticks I Bidens a L. Beggar-ticks I B oAW N oo Bgger-t cks I SiTr-hIws perroiatuv L. Cup plant I aa WiilY. Rough 5umpweed I A-mros a tr1fida L. Giant ragweedC KWE rcSla artemaiifolia L. Common ragweed A A Ambrosia bTdentata Mich .Ragweed I C AMbt0sta i Ostaichya Oc western ragweed A-D ianthitu strumarium L. Common cocklehur I A A X m-,Trtue um L. Yarrow C I I-C hgontheoWe leucanthemiM Ox-eye daisy Iroutt. Louisiana I 0 CD 0-

-C TABLE 2.2-1 (Sheet 10)0 boSo Community Type: ;- 4 bf ,~' .Scientitic Nace Common Name Index of Relative Community Abundance Compositae 1continued)

Sot id sp. L. Goldenrod 1 Soidago candensis IL. Canada goldenrod I-S Ia ruqo@aa Mill. Rough-leaved goldenrod I 0 ago ra~m itolia (L.) Saliab. Narrowleaf goldenrod I-C C-A C-A ierezia racunculoides (DC.) Blake Broomweed C-.Xser .ap. Wild aster I-C Aster ericoides L. Heath aster I-C I*Ion 5atri Osu Muhl. Rough fleabane I 1-C I Conyza (L.) Cronq. ilorseweed I S -r ur.um l. Everlasting I nttenn-ara n Greene Field pussytoes I I EunaTorlu purpuireum L. Joe-pye weed iiE~Tiilp

'Im u -osn -Ifoutt. White anakeroot I I_ _ sero _um L. Thoroughwort C C atr no tch a Mich. elazing star ncatria uook i Blazing star I I-- ic.] ata Ironweed C V"- bUa'-fn'jib r r .Ironweed I C Carsium arvenrse (L.) SCOp. Canada thistle I I Prenanthes sp. L. White Lettuce fdtraxdc:m officinale Weber a endel ion I tu sp. L. -Prickly lettuce I Latuca serriola L. Prickly lettuce I Latuca Sbennis (moench) Fern. Tall lettuce I a D = community dominant; A = abundant; C -common; I = relatively infrequent.

Table 2. Land uses within the Wolf Creek -Rose Hill transmission line right-of-way (from KGE 1975, Table 3.9-1).WCGS -ER TARLE 3.9-1 LAND USE OF THE ROSE HILL TO WOLF CREEK TRANSMISSION LINE RICGHT-OF-WAY Ldrnd Use Type Linear Feet Acreaqe Percent of Total Cropl-and Row and Broadcast Forage subtotal Grazing Pasture Wooded Pasture Ran7e Wooded Range Subtotal Woodiands Woods Riparian Woods-ledgerows Subtotal 84,405 32,390 290.7 111.5 ,17.2 6.6 116,795 402.2 23.8 20,850 4,690 299,260 19,930 71.8 1G.2 103D.5 68.6 4.2 1.0 60.9 4.1 344, 73n 1187.1 70. 2 13,500 4,190)2O 46.5 14.4 2.5 2.8 0.9 0.1 18,41.0 7,930 650 2,525 63.4 27.3 2.2 8.7 3.8 1.6 0.i 0.5 Idle Land Waterways Roads TOTALS 491,040 1690.9 100.0 Table 3. Land uses within the rerouted right-of-way section of the LaCygne -Benton 345 kV transmission line in the vicinity of Wolf Creek Generating Station.Linear Percent of Land Use Types Feet Acreage Total Cropland 7,953 27.4 19.7 Grazing 12,646 43.5 31.2 Hay meadow 4,498 15.5 11.1 Woodlands 2,021 6.9 5.0 Riparian 782 2.7 1.9 Shoreline/Wetland/Shallow water 2,216 7.6 5.5 Surface water 521 1.8 1.3 Wildlife lands (1) 6,610 22.8 16.4 Other (2) 3,194 11.0 7.9 Total 40,441 139.3 100 (1) Includes native grasses, grass-brush mix, and (2) Includes roads, gravel areas, and WCGS yard brush habitats.areas.

Table 4. Land uses within the right-of-way of the WCGS Athens 69kV transmission line.tap of the Burlington

-Linear Percent of Land Use Types Feet Acreage Total Cropland 7,041 24.2 32.9 Grazing 652 2.2 3.0 Hay meadow 3,715 12.8 17.3 Woodlands 65 0.2 0.3 Riparian 0 0 0 Shoreline/Wetland/Shallow water 978 3.4 4.6 Surface water 391 1.3 1.8 Wildlife lands (1) 5,671 19.5 26.5 Other (2) 2,933 10.1 13.4 Total 21,446 73.8 100 (1) Includes native grasses, grass-brush mix, and brush habitats.(2) Includes roads, gravel areas, and WCGS yard areas.

Literature Cited Kansas Gas and Electric Company. 1975. Wolf Creek Generating Station Unit No. 1, Environmental Report, Construction Stage.Kansas Gas and Electric Company. 1982. Wolf Creek Generating Station Unit No. 1, Environmental Report Operating License Stage.Nuclear Regulatory Commission (NRC). 1975. Final Environmental Statement (FES)for Wolf Creek Generating Station. NUREG-75/096.

Docket No. STN 50-482. Office of Nuclear Reactor Regulation, Washington, D.C.Nuclear Regulatory Commission (NRC). 1982. Final Environmental Statement Related to the Operation of Wolf Creek Generating Station Unit No. 1. NUREG-0878.

Office of Nuclear Reactor Regulation, Washington, D.C.U. S. Army Corp of Engineers, 2002. Draft Supplement to the Final Environmental Impact Statement Prepared for the : Reallocation of Water Supply Storage Project: John Redmond Lake, Kansas. Volume 1, U. S. Army Corp of Engineers.

June.

Enclosure 3 to ET 07-0001 WCGS Environmental Construction and Operational Environmental Operating Reports EA Report KGElI WOLF CREEK GENERATING STATION CONSTRUCTION ENVIRONMENTAL MONITORING PROGRAM, FEBRUARY 1981-JANUARY 1982 Prepared for Kansas Gas and Electric Company Wichita, Kansas Prepared by Ecological Analysts, Inc.221 Oakcreek Drive Lincoln, Nebraska 68528 Ronald G. King, Project'rfnager Approved by: Barry ' Smith, Director of Operations 4 June 1982

3. PHYTOPLANKTON 3.1 FIELD AND ANALYTICAL METHODS Duplicate water samples for phytoplankton analyses were collected in April, June, October, and December at Locations 1, 4, and 10 in the Neosho River and at Locations 2 and 6 in the cooling lake for Wolf Creek Generating Station (WCGS). The locations in the cooling lake were also sampled for phytoplankton in February and August. Subsampies were stored in prelabeled, 8-oz bottles containing five milliliters of IM" preservative (Meyer 1971) for later algae identification, enumeration, and biovolume determination.

Carbon fixation rate and chlorophyll a concentration were assessed from unpreserved portions of the samples and were used as indices of primary productivity.

The inverted microscope method (Lund et al. 1958; Weber 1973) was used to determine phytoplankton species composition and abundance.

Identification and enumeration of phytoplankton were performed under oil innersion at 1 ,DOOX magnification.

Approximately 400 reporting units per duplicate sample were identified to the lowest positive taxa and quantitatively reported using various reporting units: Algal form Reporting Units (units/ml)

Diatoms Each frustule Unicellular Each cell Colonial 4 cells (50 cells for some bl ue-green algae)Filamentous 100-wi lengths Taxonomic keys used as identification aids included Hustedt (1930), Huber-Pestalozzi (1941, 1961, 1968), Smith (1950), Cleve-Euler (1951), Tiffany and Britton (1952), Drouet and Daily (1956), Edmondson (1959), Prescott (1962, 1964), Patrick and Reimer (1966, 1975), Drouet (1968), Stoermer and Yang (1969), Taft and Taft (1971), and Whitford and Schumacher (1973). Additional references were consulted as needed for specific taxa.Density data were used to calculate diversity and evenness jShannon 1948 using natural logarithms; Pielou 1966; Zar 1968) at each location on each sampling date. Biovol we determinations were made following the procedures described by Cowell (1960) and Hohn (1969). Cell volumes were calculated using the geometrical configuration that best suited each taxon and expressed as microliters per liter (uI/liter).

The rate of carbon fixation was estimated with the light bottle/dark bottle C-14 method (Wetzel 1964; Parkos et al. 1969; Strickland and Parsons 1972).Six 50-ml light bottle subsamples (three per duplicate water sample) and one 50-ml dark bottle subsample per location were inoculated with 4-6 microcuries of aqueous C-14 bicarbonate solution.

Dark bottle subsamples were wrapped in aluminum foil to exclude light, and all subsamples were incubated for 3 hours3.472222e-5 days 8.333333e-4 hours 4.960317e-6 weeks 1.1415e-6 months at a constant temperature

{near ambient) and light intensity in an environmental chamber. Subsamples were filtered onto membrane filters 3-1 0 (0.45-iro pore size), which then were air-dried under dark conditions.

Upon return to the laboratory, the filters were dried further in a desiccator, fumed with concentrated HC1 for 10 minutes (Wetzel 1965), and placed in low potassium scintillation vials. After 17 milliliters of scintillation fluid were added to each vial, C-14 activity was measured with a refrigerated liquid scintillation counter. Primary produ~tivlty was expressed as carbon fixed per cubic meter of water per hour (mig C/m /hr) at each location.Six 50-ml subsamples per location (three per duplicate water sample) were filtered through a thin layer of MgCO on glass-fiber filters for chlorophyll a analysis.

Filters were placed in cantrifuge tubes and stored on ice under dark conditions, until further processing.

The filters later were eluted in 10 milliliters of 90 percent aqueous acetone for 24 hours2.777778e-4 days 0.00667 hours 3.968254e-5 weeks 9.132e-6 months at 4 C under dark conditions, then subjected to ultrasonic disruption.

After samples were clarified by centrifugatlon, their fluorescence was determined before and after the addition of IN HCI (Lorenzen 1966). Standard equations (Strickland and Parsons 1972; APHA 1976) were used to calculate chlorophyll a concentrations.

These concentrations, correcied for phaeoplgments, were reported per cubic meter of water (mg Chl a/m ) at each location.3.2 RESULTS AND DISCUSSION Phytoplankton collected in the Neosho River and WCGS cooling lake during 1981 consisted of 149 taxa representing 73 genera and 7 algal divisions (Table 3-1). More taxa were observed in the cooling lake than In the river, probably because two additional collections were made in the cooling lake and because river populations were pumped to the cooling lake (near Location 2) as the lake was filled. Of the 149 taxa identified, most (81) were common to both the river and cooling lake. The Chlorophyta (green algae), Bacillariophyta (diatoms), and Cyanophyta (blue-green algae) were well represented in phytoplankton collections (51, 38, and 25 taxa, respectively).

The total number of taxa observed in the river was very similar In 1980 and 1981 (100 vs. 103). Density and blovolume for each taxon are presented by sampling date and location in Appendix B, along with summaries of phytoplankton data for the Neosho River from 1973 through 1980.Phytoplankton standing crops, as estimated by total density, total blovolume, and chlorophyll a concentrations, were generally similar in the Neosbo River and cooling lake-(Table 3-2). An exception occurred in April when standing crops were much higher in the river than in the cooling lake. Phytoplankton productivity as estimated by carbon fixation rates exhibited spatial patterns similar to those observed for standing crop. Differences between the river and cooling lake diversity indices were generally less pronounced than those for standing crop (Table 3-3).3.2.1 Neosho River During 1981, total phytoplankton densities in the Neosho River ranged from 3,244 to 73,714/mil; total biovolumes ranged from 0.76 to 8.78 pl/l (Table 3-2). Values for both standing crop parameters were within the respective ranges observed for the river during previous studies (Tables B-l and 8-2), 3-2 TABLE 3-1 ALGAE IDENTIFIED IN PHYTOPLANKTON SAMPLES FROM THE NEOSHO RIVER AND THE COOLING LAKE OF WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1981 Neosho Cooling Taxa River Lake BACILLARIOPHYTA (Diatoms)Central es C clotella atomus Hustedt X X Cfclotell a meneghiniana Kuetzing X X Cyclotella stelligera (Cleve and Grunow ex Cleve)VanHeurck

-X Melosira ambtgua (Grunow) 0. Mueller X X Melosira distans <Ehrenberg)

Kuetzing X X Melosira granulata (Enrenberg)

Ralfs X K Melosira varians C.A. Agardh X -Skeletonema potamos (Weber) Hasle -X Stephanodiscus astraea (Ehrenberg)

Grunow X X Stephanodiscus hantzschii Grunow X X Stephanodiscus.

Invisitatus Hohn and Hellerman X X Stephanodiscus niagarae Ehrenberg X X Stephanodi scus spp. -x Small unidentified centrics X X Pennales Achnanthes minutissima Kuetzing -X Cocconeis placentula Ehrenberg X -Fragilaria capucina DesMazieres

-x Frac ilaria construens (Ehrenberg)

Grunow -X Fragilaria vaucheriae (Kuetzing)

Petersen -X Gomphonema parvulum (Kuetzing)

Kuetzing -X Navicula capitata Ehrenberg X -Navicula cr a ocephala Kuetzing X X Navicula rhynchocephala Kuetzing K -Navicula spp. X X Nitzschia acicularis (Kuetzing)

W. Smith X X Nitzschia captellata Hustedt X -Nitzschia dissipata (Kuetzing)

Grunow X x Nitzschia fonticola Grunow X x Nitzschia frustulum (Kuetzing)

Grunow X -Nitzschia 1ng. issima (Brebisson)

Ralfs X X Nitzschia palea (Kuetzing)

W. Smith X X Nitzschia subcapitellata Hustedt X X Nitzschia tryblionella Hantzsch X X Nitzschia spp. X X Rhoicosphenia curvata X X Synedra runpens X X Synedra ulna X -Synedra spp. -x 3-3 TABLE 3-1 (.CONT.)Neosho Cooling 0 Taxa River Lake£ CHLOROPHYTA (Green Algae)Actinastrum hantzschii Lagerheim X X Ankistrodesmus convolutus Corda -x Ankistrodesmus falcatus (Corda) Ralfs X X Carteria cordifornis (Carter) Deising X X Carteria spp. -x Chiramydonmonas spp. X X Chlorooqnium elongatum Dangeard X -Chorogonlum spp. X -Closteriopsis longissima Lemmermann X X Coelastrum microporun Naegeli ex A. Braun X X Cosmarium spp. X x Crucigenia quadrata Morren X K Crucigenia tetrapedia (Kirchner)

West and G.S. West X X Dictyosphaerium ehrenbergianum Naegeli X X Dictyosphaerlium UIchelilu i Wood X X Erakatothrix 9elatinosa Wille X x Gloeocyst1s gigas (Kuetzing)

Lagerheim X X Goenkinia r-a--i-ta (Chodat) Wille x x Kirchneriella subsolitaria G.S. West X X Lagerheimla quadriseta (Lemmernann)

G.M. Smith X X Micractinlum pusi1um Fresenius X x Ne hrocytium agardhianum Naegeli -x Oocystis b Snow X X Oocystis =loeocystiformis Borge X X Oocystis pusilla Hansgirg X X Pandorina morum (Mueller)

Bory -X Pediastrum boryanum (Turpin) Meneghini K -Pediastrum duplex Meyen X x Pediastrum tetras (Ehrenberg)

Ralfs X -Quadrigula lacustris (Chodat) G.M. Smith X -Scenedesmus abundans (Kirschner)

Chodat x X Scenedesmus acuninatus (Lagerheim)

Chodat X -Scenedesmus arcuatus Lemmermann

-X Scenedesmus biýa (Turpin) Lagerhaim X x Scenedesmus dimorphus (Turpin) Kuetzing X X Scenedesmus us Meyen -X Scenedesmus cuadricauda (Turpin) Brebisson X X Scenedesmus spp. X -Schroederia setigera (Schroeder)

Lemmennann X x Selenastrum gracile Reinsch -X Selenastrum minutum (Naegeli)

Collins X X Selenastrun westii G.M. Smith -X Sphaerocystis schroeteri Chodat -X Staurastrwn spp. X X 3-4 TABLE 3-1 (CONT.)Neosho Cooling Taxa River Lake Tetraedron minimum (A. Braun) Hansglrg X -Tetraedron muticum (A. Braun) Hansglrg X X Tetraedron regulare Kuetzlng -X Tetrastrun staurogentaeforme (Schroeder)

Lemmermann

-X Treubaria setigerum (Archer) G.M. Smith -X Trochi sct azchadrii aslt Lemmermann

-X Unidentified coccoid greens X X CHRYSOPHYTA (Yellow-green Algae)Arachnochl.oris minor Pascher x X Chromulina spp. X X Chrysochromullna spp. X X Chrysococcus punctiformis Pascher X X Chrysococcus spp. -X Dinobryon bavaricun Imhof -X Dtnobryon sertularia Ehrenbeg -X Mallomonas akrokomas Ruttner -X Mal 1 omonas spp. -X Ochromonas spp. -X Ophocytium capitatum Wolle X X Pseudokephrion spp. -X Unidentified chrysophyte X X CRYPTOPHYTA (Cryptomonads)

Chroomonas spp. -X Cryptomonas spp. X X Rhodomonas minuta Skuja X K CYANOPHYTA (Blue-green Algae)Anabaena spp. -X A hanocapsa delicatissima West and West X X Aphanocapsa el achista West and West X X A hanocapsa spp. X K Aphanothece nidulans P. Richter X X Aphanothece spp. -X Chroococcus dtpesus (Keissler)

Lemmermann X X Chroococcus m 7norVKuetzing)

Naegeli X x Chroococcus prescott1i Drouet and Daily ex Drouet X Chroococcus spp. -X Coelosphaerium kuetzlngianum Naegeli X X Coelosphaerium pal lidum Lemmermann X X Gloeocapsa punctata Naegell -X Gloeocapsa spp. X -Gloeothece linearis Naegell -X Gloeothece i 0le (Kuet zing) Rabenhorst X X 3-5 a i6 TABLE 3-1 (CONT.)Neosho Cooling C) Taxa -River Lake Gomphosphaeria lacustris Chodat X X Lyngbya diguetil Gomont ex Harlot X X Lyngbya taylorii Drouet and Stuckland X -Lynqbya spp. X -Marssoniella elegans Lemmermann X X Merlsmopedia tenuissima Lemermann X X I Microcystis incerta Lemmemann X X Unidentified blue-green coccoids x x Unidentified blue-green filaments

-X EUGLENOPHYTA (Eug 1 enol d s)Euqlena deses Ehrenberg X X Eiqlena gra-clis Klebs X X Euqlena minuta Prescott -X Euglena proxtma Dangeard -X Euglena tripteri s (DuJardin)

Klebs X -Lepocinclis acuta Prescott ex Prescott, Silva, and Wade X X Phacus acuminatus Stokes -X Phacus caudatus Huebner X -Phacus spp. X X Trachelomonas hispida (Perty) Stein -X Trachelomonas robusta Swirenko X -Trachelomonas schauinslandii Lemmermann X X Trachelomonas volvocina Ehrenberg

-X 4 Trachelomonas spp. -X PYRRHOPHYTA (Dinoflagel lates)Ceratium hirundinella (O.F. Mueller) DuJardin -X Glenodiniwn spp. -X Gymnodini um spp. X X Peridinium spp. X -Unidentified Microflagel 1 ates -X Total Taxa 103 127 149 3-4 I LI TABLE 3-2 STANDING CROP AND PRODUCTIVITY OF PHYTOPLANKTON IN THE NEOSHO RIVER AND THE COOLING LAKE OF WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1981 tl Sampling Locations Neosho River Cooling Lake Parameter/Sampl ing Date 1 4 -6 Total Density (units/m1) (a)24 FEB 81 ---38,449 e4,627 28 APR 81 37,322 67,182 73,714 20,635 3,170 23 JUN 81 5,394 4,579 3,244 8,506 6,895 25 AUG 81 ---22,356 25,133 20 OCT 81 12,706 9,873 10,645 18,887 16,073 15 DEC 81 7,469 6,625 9,792 30,908 13,997 Total Biovolume (ul/liter) 24 FEB 81 ---3.53 1.78 28 APR 81 4.91 8.78 7.79 2.60 0.85 23 JUN 81 1.36 1.29 0.76 2.14 0.52 25 AUG 81 ---2.22 0.99 20 OCT 81 3.68 2.73 2.85 1.85 1.93 15 DEC 81 2.74 2.42 4.42 5.26 2.64 Chlorophyll a Conjentration (mg ChO a/mr)24 FEB 81 ---25.49 10.20 28 APR 81 30.57 44.28 33.74 11.50. 4.92 23 JUN 81 9.28 10.20 10.85 10.33 2.73 25 AUG 81 ---11.50 6.01 20 OCT 81 13.71 18.33 21.52 14.45 6.99 15 DEC 81 17.28 14.30 15.87 16.50 11.15 Carbon Fixation Rate (mn C/m /hr)24 FEB 81 .- -115.76 40.20 28 APR 81 18.39 30.42 22.39 4.07 2.83 23 JUN 81 22.58 21.64 23.15 24.50 5.18 25 AUG 81 ---2.80 2.39 20 OCT 81 13.81 14.34 13.80 14.76 7.05 15 DEC 81 48.90 40.09 43.35 32.33 23.87 (a) Sampling in the Neosho River was not scheduled for February and August.3-7 TABLE 3-3 DIVERSITY INDICES FOR PHYTOPLANKTON IN THE NEOSHO RIVER AND THE COOLING LAKE OF WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1981 Sampli in Locations ( Neosho River Cooling Lake Indexa, /Samplinq Date 1 10 4 2 6 Species Diversity(b) 24 FEB 81 _(c) --2.46 2.07 28 APR 81 2.90 2.93 .2.78 2.77 1.42 23 JUN 81 3.10 3.12 3.02 3.08 2.43 25 AUG 81 ---2.48 2.15 20 OCT 81 2.70 2.84 2.86 2.91 2.87 15 DEC 81 2.59 2.49 2.31 1.98 2.66 Evenness Value(d)24 FEB 81 ---0.66 0.60 28 APR 81 0.77 0.78 0.75 0.67 0.44 23 JUN 81 0.79 0.81 0.81 0.80 0.70 25 AUG 81 ---0.65 0.58 20 OCT 81 0.72 0.76 0.77 0.79 0.73 15 DEC 81 0.76 0.75 0."69 0.60 0.73 Number of Taxa 24 FEB 81 ---42 31 28 APR 81 44 42 40 63 25 23 JUN 81 51 46 41 46 32 25 AUG 81 ---44 40 20 OCT 81 43 41 40 40 50 15 DEC 81 30 28 29 28 39 (a) Combined results of duplicate analyses.(b) Based on Shannon (1948) using natural logarithms.(C Sampling in the Neosho River was not scheduled for february and August.Zar (1968).3-8 even though the method of analysis was changed from modified Lackey microtransects (Lackey 1938; Mackenthun 1969) to the inverted microscope 2:, beginning in 1980. Densities and biovolumes in 1981 were generally similar at upstream (1) and downstream locations (4 and 10) except in April when downstream values were approximately twice those observed upstream.

Most of the spatial and temporal differences in phytoplankton appeared related to the flow regime in the Neosho River. Highest values and greatest spatial differences occurred in April when flows were lowest; reduced values and spatial differences in remaining collections corresponded to flows greater than 1,000 cfs (Table 2-2).No single algal group numerically dominated the river phytoplankton in 1981 although diatoms (primarily centrics) always composed at least 30 percent of total numbers (Table 3-4). Green algae, cryptomonads, and blue-green algae composed at least 20 percent of total density at various times during the year, and these groups were considered seasonal codominants with diatoms.Green and blue-green algae codominated (with diatoms) in April and June, blue-green algae codominated in October, and cryptomonads codominated in December.

Biovolumes were more clearly dominated by diatoms, but green and blue-green algae were relatively important in April, as were cryptomonads in June and December.

Yellow-green algae, euglenoids, and dinoflagellates were minor components of the river phytoplankton, both in terms of density and biovol ume.Twenty phytoplankton taxa were considered important in the river because they composed at least 5 percent of total density or biovolume during 1981 (Table 3-5). Important centric diatoms included species of CUclotella, Melosira, and Stephanodiscus, as well as small forms (<5-um diameter) that could not be positively identified by the analytical technique employed.

Previous studies have attributed importance of centric diatoms in this portion of the Neosho River to water releases from John Redmond Reservoir because large populations of these diatoms are generally characteristic of lentic (lake) habitats (Repsys 1979a; Bockelman 1980a). Downstream declines in the abundance of several taxa have often provided additional evidence of their origin In the reservoir.

However, of the five centric taxa important on 28 April 1981, only Stephanodiscus astraea declined in abundance at the downstream locations.

The other cent rics ehibiTted dramatic increases at Locations 4 and 10 that seemed to indicate they either originated in the river (e.g., Stphanodiscus hantzschii) or were well adapted to the lotic (flowing) habitat of the Neosho River (e.g., Cyclotella atomus, C. meneghiniana and small unidentified centrics).

The low flow conditions in April may have contributed to population increases of some taxa by creating more lentic (pool) habitats within the river.Many important nondiatoms also exhibited downstream increases in April. These taxa included Chlamydomonas spp. (Chlorophyta), Chrysochromulina spp.(Chrysophyta), Crptomonas spp. and Rhodomonas minuta (both Cryptophyta), and Chroococcus dispersus (Cyanophyta).

The larger downstream populations of the relati vely fragile flagel 1 ates Chlamydomonas, Chrysochromulina, and Rhodomonas provided additional evidence that quiet, pool-like areas existed near Locations 4 and 10 in April because these taxa typically exhibited downstream 3-9 TABLE 3-4 MEAN DENSITY AND BIOVOLUME OF MAJOR ALGAL GROUPS IN PHYTOPLANKTON OF THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1981.Density at Sampling Locations

-.6iovolume at Sampllng Locations Date 28 APR Taxa Bactllariophyta (Centrales)(Pennales)

Chlorophyta Chrysophyta Cryptophyta Cyanophyta Eug1enophyta Pyrrhophyta

=1 Units/m1 z unitslml % ~n f ter 0 vl/liter I-J 0 23 JUN Bacillarlophyta (Centrales)(Pennales)

Chlorophyta Chrysophyta Cryptophyta Cyanophyta Euglenohyta Pyrrhophyta 20 OCT Bacillariophyta (Centrales)(Pennales)

Chlorophyta Chrysophyta Cryptophyta Cyanophyta Euglenophyta Pyrrhophyta 15 OEC Bacillartophyta SCentrales)(Pennales)

Chlorophyta Chrysophyta Cryptophyta Cyanophyta Euglenophyta Pyrrhophyta 14,307 13,3191 989)11,472 1,047 1,047 9,274 174 0 1,774 S1.590)184)1,328 58 795 1,400 29 10 4,829 4524)(305)1,751 23 1,433 4,657 12 0 2,736 S2,683~783 767 1,983 1,147 13 0 38.34 35.691 2.65)30.74 2.81 2.81 24.85 0.47 0.00 32.89 29.471 3.41)24.62 1.08 14.74 25.96 0.54 0.18 38.01 (35.60)2.401 13.78 0.18 11.28 36.66 0.09 0.00 36.64 35.931 0.71)10.49 10.26 26.55 15.36 0.18 0.00 30,069 29.022)1,047)17,463 2,792 4,188 12,381 291 0 1,910 1,6581 252)964 39 553 1.075 39 0 3.513 S3,149)364)1,733 47 1,6?I 2,959 0 0 2,802 I 2.736j 66)691 542 1,599 977 0 13 44.76 43 20)1:56)25.99 4.16 6.23 18.43 0.43 0.00 41.70 136.701 5.50)21.06 0.85 12.07 23.48 0.85 0.00 35.58 31.891 3.69)17.55 0.48 16.42 29.97 0.00 0.00 42.30 S41.301)1.00)10.43 8.18 24.14 14.75 0.00 0.20 31,174 30,7671 407)14,787 4,420 4,188 19,145 0 0 989 882j 107 960 48 388 860 0 0 3.795 3,501)294).1,815 153 1,786 3.096 0 0 4,256 S4,2171 40)1,001 357 2,697 1,454 13 13 42.29 41.741 0o.55)20.06 6.00 5.68 25.97 0.00 0.00 30.48 27.191 3.29)29.58 1.49 11.95 26.49 0.00 0.00 36.65 32.89)17.05 1.43 16.78 29.08 0.00 0.00 43.47 43.061 0.40)10.23 3.64 27.54 14.85 0.13 0.13 2.42 S2.281 0. 14)1.28 0.06 0.37 0.50 0.28 0.00 0.74 0.70)0.04)0.19 (0.01 0.27 0.03 0.07 0.05 3.24 3 320)0.04)0.16 (0.01 0.19 0.06 0.03 0.00 1.37 1 1311 0.06)0.13 0.05 1.11 0.02 0.03 0.00 49.32 46.48)2.84)26.08 1.22 7.57 10.18 5.63 0.00 54.68 51.621 3.o6)14.12 0.36 20.02 2.39 4.96 3.47 87.93 86.88)1.05)4.41 0.05 5.04 1.75 0.81 0.00 50.06 47.97)2.09)4.78 1.94 40.46 0.76 1.05 0.00 3.78 I 3 62)0:16)1.71 0.14 2.25 0.28 0.62 0.00 0.67 0 .59)0.08)0.18 (0.01 0.28 0.02 0.11 0.00 2.27 0:09191 0.20 (0.01 0.21 0.04 0.00 0.00 1.41 001.411 0.11 0.04 0.84 0.02 0.00 (0.01 43.03 41.241 1.79)19.49 1.59 25.69 3.18 7.02 0.00 52.82 46.49j 6.33)14.35 0.28 22.02 1.69 8.84 0.00 83.11 79.96j 3.161 7.47 0.15 7.66 1.61 0.00 0.00 58.07 58.04)0:04)4.54 1.60 34.76 0.67 0.00 0.36 3.93 I 3.87)0.06)1.54 0.24 1.58 0.50 0.00 0.00 0.49 0.46)0.02)0.11 (0.01 0.14 0.01 0.00 0.00 2.40 2 233j 0.07)0.16 0.01 0.23 0.05 0.00 0.00 2.45 S2.45)0.15 0.02 1.74 0.02 0.04 (0.01 50.44 49.631 0.80)19.81 3.14 20.24 6.37 0.00 0.00 64.49 61.39j 3:10)14.31 0.59 19.04 1 .58 0.00 0.00 84.15 (81:62j (2.53)5.76 0.41 8.08 1.60 0.00 0.00 55.35 55.34)0:01)3.33 0.52 39.24 0.51 0.86 0.20 Note: Values for centric relative importance and pennate diatoms are presented separately in parentheses because of their in phytoplankton.

ft a lb a a a A

-in -ncm s. 1 .~ .~Is 1 -. t~ 1I- rrn f~\ N-. -~ ~......4 TABLE 3-5 MFAN DENSITY (inits/m1)

OF MRTANr TAXA IN PHYIORAWYON AT LOCATIONS 1, 10, AN) 4 IN THE NEOSHO RIWR WEA IfLF a M GEERATING STATION, BLIU M, KANSAS, 1981 28APR 23JUN 20OCT 15DEC Taxa 1 10 4 1 10 4T 1 10 4 O Bac11ariooityta Centrales lotella ataus 1,8)3 5,525 6,107 39 58 0 576 435 352 93 172 13 lJniaim 640 5,002 5,700 136 165 19 364 329 317 317 251 330 ellosira distans 0 0 0 330 252 165 94 94 0 172 0 66 Meloslrangramulata 0 0 0 271 417 223 59 82 82 0 0 0 Me o! a varlans 0 0 0 0 0 19 0 0 0 0 0 0 SRFOhardisq-us astraea 5,176 2,908 3,141 582 572 397 2,315 1,588 1,704 1,044 1,269 2,075 te 1anscus tz i 0 3,(24 4885 0 0 0 0 0 0 0 0 0 Stepandi scus tarae 0 0 0 19 0 0 0 0 0 0 0 0 rall ulmtidentfled centrics 5,700 12,272 12,388 213 223 58 928 587 1,046 912 1,018 1,454~Chlorophlyta"iwt 174 3,6 2,734 10 1910 12 23 35 264198 159 Dilctyosp!ertlun pulW eln 2,574 2,559 1,817 6B 44 155 502 520 488 225 188 380 Kircthertalla subsolitarla 3,417 2,414 3,09 405 189 330 200 214 320 43 50 76 131 73 44 0 7 10 9 0 6 0 0 3 Chrysophyta Ch ýYhyult aspp. 872 2,2M8 3,548 10 0 0 0 0 23 687 463 357 cryptopiyt C targSOspp.

349 2,305 1,512 194 233 107 576 764 881 1,428 1,057 2,366 f8 1,803 2,675 6)1 32 281 858 858 905 555 542 330 Cy dsqss4,057 7,619 14,424 0 58 76 0. 41 117 430 380 479 ri atnusif 0 C) 0 480 582 32D 1,618 887 1,075 0 26 C)25s 27 372 470 35 24 17 585 488 591 24 10 21 Unidentiflie blue-green ds 3,417 3,737 2,835 632 333 3D8 2,271 1,436 1.092 677 539 952 Note: Taxa cwpostng at least 5 percent of total iOytoplankton density or biovoluhe at any location on any date vere considered itportant.

1 reductions during periods of high river flow. Other important nondiatoms had inconsistent spatial distribution (e.g., the blue-green alga Merismopedia tenuissima) or had higher densities at Location 1 (e.g., the green alga i) IKi rchneri ellIa subsol itarl a).Annual maxlmwn phytoplankton abundance has occurred in a different month during almost every year since 1973. The effects of physical factors such as water temperature, light intensity, and day length that normally control seasonal algal cycles were modified by seasonal differences In discharge regimes from John Redmond Reservoir (Bockelman 1980a). Annual climatic differences In length and severity of winters, and the pattern and amount of yearly rainfall were factors that indirectly affected phytoplankton densities in the Neosho River because they influence turbidity, retention time, and algal standing crops in the reservoir.

Wilde and Reetz (1976) and Farrell (1978) associated increases in phytoplankton densities and chlorophyll a concentrations with concurrent declines in annual precipitation within The John Redmond Reservoir watershed from 1973 to 1976.During 1981, chlorophyll a concentrations ranged from 9.28 to 44.-8 mg Chl a/m , and carbon fixatiof-rates ranged from 13.80 to 48.90 mg C/m /hr (liable 1-2). Previous ranggs in the Neosho River were 0.70 -50.96 mg Chl a/m and 0.15 -130.65 mg C/m /hr (Tables B-4 and 8-5). In the present study, chlorophyll a concentrations were highest in April, whereas carbon fixation rates were greatest in December.

The chlorophyll peak corresponded to large phytoplankton densities and biovolumes in April. There was no apparent reason for the December peak in carbon fixation rates. All standing crop estimates at that time were well below the yearly peak values, and water temperatures were seasonally low (approximately 3.0 C). Spatial and temporal differences in diversity indices were minor in the river (Table 3-3), and all values for Shannon's Diversity Index were within the previously reported range (0.19 -3.34) for phytoplankton in the Neosho River (Table B-3).As in previous years, no adverse effects of WOGS construction on phytoplankton composition, abundance, diversity, biovolume, chlorophyll a standing crop, or carbon fixation rate were evident in the Neosho River. Values for these parameters have generally been similar at all three river locations and especially at Locations 10 (upstream from Wolf Creek) and 4 (downstream from Wolf Creek). Wolf Creek, the drainage system through which most runoff from construction-related activities is carried to the Neosho River, has exhibited intermittent flow throughout the monitoring studies and has had only minor effects on flow regimes and phytoplankton in the river. Closure of the WCGS cooling lake main dam was completed in September 1980, and no flow was observed in Wolf Creek during 1981. As previously stated, phytoplankton communities in this portion of the Neosho River have been influenced most directly by water releases from John Redmond Reservoir.

3.2.2 Cooling Lake Total phytoplankton densities in the cooling lake ranged from 3,170 to 38,449/ml in 1981; total biovolumes ranged from 0.52 to 5..26 pl/l (Table 3-2).Values for both parameters were generally greater at Location 2 than at 3-12 Location 6. Densities at Location 2 exceeded 30,000/ml in february and December, whereas densities at Location 6 were greater than 20,000/ml in February a~d August. Chlorophyll a concentrations ranged from 2.73 to 25.49 mg Chl a/m and were always greater at Location 2. Carbon fixation rates were alsS consistently greater at Location 2 and ranged from 2.39 to 115.76 mg C/m /hr (Table 3-2). On most sampling dates, phytoplankton communities in the cooling lake were moderately diverse. However, diversity values were less than 2.00 in April (1.42 at Location 6) and in December (1.98 at Location 6, Table 3-3).Numerical dominance of cooling lake phytoplankton by major algal groups varied both temporally and spatially in 1981. Diatoms (primarily centrics), green algae, and blue-green algae codominated at both locations in February (Table 3-6). Blue-green algae codominated with four other groups at Location 2 in April, whereas cryptomonads clearly dominated total phytoplankton nunbers at Location 6. In June, diatoms ,green algae, cryptomonads, and blue-green algae were present at approximately equal densities at Location 2, but blue-green algae alone dominated the phytoplankton at Location 6. Blue-green algae were most important in terms of relative abundance at both locations in August, and their numerical dominance, although somewhat reduced, continued in October when diatoms and green algae again became relatively important.

The cooling lake in December was composed primarily of yellow-green algae with diatoms and green algae continuing to be relatively important at Location 6. Similar temporal and spatial variations in the relative contribution of these five algal groups were evident for phytoplankton biovolumes, but for this standing crop parameter, the relative importance of diatoms and cryptomonads generally increased while that of yellow-green algae and blue-green algae was often reduced (Table 3-6).During the present study, 28 taxa were considered important constituents of the cooling lake phytoplankton because they composed at least 5 percent of total density or biovolume (Table 3-7). For some taxa (e.g., Ankistrodesmus falcatus and Rhodomonas minuta), density differences between the two locations were less pronounced than was expected from the differences observed in major group densities.

However, several taxa (e.g., Melosira distans, Stephanodiscus astraea, Di ctosphaeri um ,pulchellun, Chrysochromulina spp., and unidentified coccoid blue-greens) were restricted to Location 2 or were much more abundant there, especially during the first half of the study. This spatial trend disappeared or was reversed for some of these taxa in the second half of 1981. Blue-green algae (e.g., Chroococcus dispersus, Marssoniella elegans, and Merismopedia tenuissima) attained maximum abundance at Loca n 6.Distinct phytoplankton communities were initially apparent at Location 2 and 6 in the cooling lake. In April and June, not only were standing crops reduced at Location 6, but diversity values were also lower. There was little evidence that these differences resulted from spatial variations in water quality (Tables 2-3 through 2-7). Instead, most of the variation in phytoplankton probably resulted from the combined effects of differences in water depth (shallow at Location 2 vs. relatively deep at Location 6) and introductions of river {Location

1) populations near Location 2 through the 3-13 4 TABLE 3-6 MEAN DENSITY AND BIOVOLUME OF MAJOR ALGAL GROUPS IN PHYTOPLANKTON OF THE COOLING LAKE OF WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1981 Density at Sampling Locations Btovolune at Samwltn Locations Date Taxa 24 FEB BactlIartophyta (Centrales)(Pennal es)Chloroplyta Chryso Iyta Cryptophyta Cyanophyta fugl enophyta Pyrrhophyta 28 APR Bacillarlophyta (Centrales)(Pennales)

Chloroplyta Chrysophyta Cryptophyta Cyanophyta Eug1 enolhyta Pyrrholhyta 23 JUN Bacillartoayta (Centrales)(Pennales)

Chlorophyta Chryso phyta Cryptophyta Cyanophyta Pyrrho 25 AUG Bacillarloayta (Centrales)(Pennales)

Chlorophyta Chrysophyta Cryptophyta Cyanophyta Eugl enopiyta Pyrrhophyta 20 OCT Bacillarto yta (Centrales)(Pennales)

Chloroehyta Chrysolhyta Cryptophyta Cyanophyta Eug1 enophyta Pyrrhophyta 15 DEC Bacillarlo yta (Centrales)(Pennales)

Chlorophyta Chrysophyta Cryptopiyta Cyano phyta Euglenophyta Pyrrhophyta a L 2 n 6 %alsM 2=i/lter S Oi/liter %13.243 (11,4231 1,819 16,613 477 477 7,549 0 0 3,203 3:164)39)2,871 3,051 3,238 8,144 81 31 ((2,147 1,853)294)2,036 128 1,994 2,099 102 0 34.44 29.71)4.73)43.21 1.24 1.24 19.63 0.00 0.00 15.52 (15.33)0.19)13.91 14.79 15.69 39.47 0.39 0.15 25.25 (21.79)(3.46)23.93 1.50 23.44 24.68 1.20 0.00 15.05 (12452)(e.s3)5.06 20.95 9.42 49.23 0.14 0.14 22.33 (21.22)(1.21)21.01 1.54 11.44 43.58 0.11 0.00 14.27 (13.80)0.47)15.96 50.49 15.99 2.97 0.31 0.00 4,683 (4,504)179)9,253 895 209 9,259 30 0 74 ( 62)( 12)206 237 2,582 44 0 0 318 277)( 41)798 128 1,008 4,643 0 0 1,084 79)2,002 1.269 846 19,912 0 0 2,986 (2,772)213)3,577 271 1.435 7,707 58 19 3.490 (3,362)127)3,312 4,398 1.618 1.034 36 109 19.01 18.29)0.73)37.57 3.63 0.85 37.60 0.12 0.00 2.32 1.96)0.37)6.51 7.46 81.47 1.38 0.00 0.00 4.61 (4.02)0.59)11.57 1.86 14.61 67.34 0.00 0.00 4.31 (4.00)(0.32)8.05 5.05 3.37 79.23 0.00 0.00 18.58 417.25)(1.33)22.25 1.69 8.93 47.95 0.36 0.12 24.93 (24.02)0.91)23.66 31.42 11.56 7.39 0.26 0.78 1.76 I 1.55)0.22)1.38 0.11 0.06 0.21 0.00 0.00 0.53 0.53)(0.01)0.31 0.31 1.05 0.23 0.10 0.08 1.00 0.94)(006 0.26 (0.01 0.62 0.03 0.23 0.00 1.09 1.03)0.06)0.17 0.10 0.56 0.07 0.04 0.18 0.82 0.82)(<0.01)0.34 0.02 0.58 0.06 0.04 0.00 49.86 43.76)6.11 39.16 3.13 1.72 5.93 0.00 0.00 20.55 (20.37)0.18)11.82 11.74 40.43 8.65 3.76 2.97 464.2 143.93)2.69)12.01 0.43 29.12 1.30 10.62 0.00 49.15 (46.471 7.86 4.64 25.01 3.13 2.01 8.20 44.47 (44.17)0.30)18.25 0.84 31.43 3.06 1.95 0.00 17.59 (17.16)0.43)6.74 21.37 51.34 0.28 2.68 0.00 0.74 I 0.73)0.01)0.72 0.02 0.03 0.23 0.01 0.00 (0.01 (<0.01)(<0.01)0.03 0.01 0.78 0.02 0.00 0.00 0.09 0.07)0.02)0.06 (0.01 0.27 0.09 0.00 0.00 0.44 0.42)(0.01 0.21 0.03 0.17 0.14 0.00 0.00 0.60 o.59)<0.01)0.48 0.01 0.33 0.06 0.07 0.38 0.87 0.86)((0.01)0.29 0.31 0.90 0.02 0.06 0.18 41.77 41.14!0.62)40.32 1.40 1.82 12.73 0.64 0.00 1.16 0.82)(0.34)3.28 1.50 91.24 2.16 0.00 0.00 16.61 (13.30)3.31)12.08 0.99 52.57 17.76 0.00 0.00 43.90 142.40)1.45)21.60 2.75 17.43 14.32 0.00 0.00 31.01 (30.62)0.39)24.70 0.71 16.89 3.06 3.77 19.84 32.99 (32.63)(0.36)10.86 11.61 34.23 0.94 2.41 6.96 3,364 S2.*798)566)1,132 4,684 2,106 11,007 31 31 ((4,217 3,988)288)3,967 291 2,160 8,231 21 0 I 4,410 4,265)145)4,931 15.606 4,944 919 97 0 (0.92 0.90)0.02)0.35 1.12 2.70 0.01 0.14 0.00 I Note: Values for centric and pennate diatoms are presented separately in parentheses because of their relative importance in iphytoplankton.

3-14

~-rrrrrTh ) C)h Ii *-S TABLE 3-7 MEAN DENSITY (units/ml)

OF IMPORTANT TAXA IN PHYTOPLANKTON AT LOCATIONS 2 AND 6 IN THE COOLING LAKE OF WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1981 24 FEB 24 APR 23 JUN 225 AUG 20 OCT 15 DEC Bac I IIariophyta Centrales Clotella atomus 60 30 648 0 0 14 157 26 2,389 1,532 824 418 a meneghtniana 0 0 295 0 243 68 314 53 166 116 679 545 loira dtstans 239 0 0 0 435 0 1,446 582 104 78 97 200 el-si-ra granulata 0 0 0 0 268 14 252 264 0 19 97 0 Stejai -'41scus asj e 1,163 298 1,136 8 626 54 189 0 665 485 485 327 a araane 0 0 0 0 38 0 0 0 0 0 0 0 Stephanodisusspp.

9,186 0 0 0 0 0 0 0 0 0 0 0 Small'uwidentifted centrtcs 328 3,937 1,027 54 179 128 314 26 623 543 2,036 1,563 Chlorophyta nktreus fcatus 7,814 6,055 407 31 89 81 189 53 561 485 1,890 382 rAn t rage 149 239 477 15 16 83 94 53 327 228 606 900 a 1,864 60 16 20 0 0 0 0 436 300 170 704 erurvo n h iiiim jjc ! 3,713 753 318 39 278 139 63 1,216 706 911 1,575 700 enrie1]a susoltar a 336 306 567 0 604 132 86 139 769 533 109 186 oc stis -usilT 485 537 100 0 131 12 63 112 166 39 145 105 S r.a--etga 0 0 12 23 0 304 0 53 0 0 0 0 Unidnified COCCOtd greens 261 104 70 5 185 19 118 172 31 199 145 73 Chrysophyta CbrysQch~omu.1DQa spp. 149 775 2,260 19 51 128 4,684 1.190 145 136 15,315 4,217 Halljgs j krokomos 0 0 23 190 0 0 0 0 0 0 0 0 Cryptophyta Crytorpnnas spp. 209 60 954 682 422 169 503 106 291 136 3,441 1,163as minuta 209 149 2,284 1,900 1,572 839 1,603 740 1,869 1,299 1.502 454 cyanop yti hrccocua disperss 4,951 8,411 5,471 5 200 1,266 126 258 846 1,081 339 336 eocpsa un tata 0 0 0 0 0 439 0 0 0 0 0 0 MrjsonIe]

e a n 0 0 97 0 179 1,506 0 192 717 78 0 0 Kerisfoedi flnu ssima 0 0 0 0 955 108 2,853 6,992 2,654 1,565 0 55 Unidenttfied blue-green coccolds 1,737 693 1,284 0 518 1,033 6,052 8,869 2,576 3,742 557 427 Euglenophyta Trarhieo!oneshispida 0 0 0 0 51 0 0 0 0 0 0 0 Pyrrhophyta Craj hirundtnella 0 0 0 0 0 0 0 0 0 19 0 0 G liium spp. 0 0 31 0 0 0 31 0 0 0 0 109 Note: Taxa composing at least 5 percent of total phytoplankton density or biovolume at either location on any date were considered important.

II U makeup water pumphouse.

Taxa, such as S. astraea, that have historically been important at Location 1 in the river (eJg., Table 3-5) and probably originate L) in John Redmond Reservoir were much more abundant early in 1981 at Location 2 than at Location 6 in the cooling lake. As the year progressed, phytoplankton differences at the two locations became less distinctive because, as the cool i ng la ke fi I ed, makeup water from the ri ver composed a 1 ower proporti on of the total lake volume and because introduced river taxa had become established in the cooling lake. Additionally, Location 2 was in the old Wolf Creek channel that was not completely inundated until October. For these reasons, the data from Location 6 probably more accurately represented typical phytoplankton of the cooling lake.3.3 SUIMIARY AND CONCLUSIONS During 1981, 149 taxa representing 73 genera and 7 algal divisions were identified in phytoplankton samples from the Neosho River and WCGS cooling lake. The Chlorophyta (green algae), Bacillarlophyta (diatoms), and 4 Cyanophyta (blue-green algae) were well represented in the collections

<51, 38, and 25 taxa, respectively).

Total densities and biovolumes in the river were within the respective ranges observed for the river during previous studies. Highest values and greatest differences between river locations occurred in April when flows were lowest;reduced values and spatial differences in June, October, and December corresponded to flows greater than 1,000 cfs. Diatoms, green algae, cryptomonads, and blue-green algae were numerically important at various times during the year, but no single algal group clearly dominated the phytoplankton on any sampling date.Most of the twenty taxa considered important in the river have historically been important and probably originated in John Redmond Reservoir.

This was especially true at Location 1. Many taxa exhibited a downstream increase in abundance during the low flow conditions of April, which seemed to indicate that quiet, pool-like areas existed near Locations 4 and 10. In other months, densities of important taxa generally declined at the downstream locations, as has often been observed in previous studies.Chlorophyll a concentrations, carbon fixation rates, and diversity values In 1981 were alio within previously reported ranges for river phytoplankton.

There was no apparent explanation for the unusual December peak in carbon fixation rates. No major differences in any parameter existed between Location 10 (upstream of Wolf Creek) and Location 4 (downstream of Wolf Creek). As in previous years, no adverse effects of WCGS construction were evident in phytoplankton composition, abundance, diversity, biovolume, chlorophyll a standing crop, or carbon fixation rate in the Neosho River.Construction effects were not expected in 1981 because most construction had been completed and because there was essentially no discharge from Wolf Creek into the river during the year.Phytoplankton populations In the cooling lake exhibited spatial and temporal variability, especially during the first half of 1981. Values 3-16 4 9 for most parameters (including diversity) were generally greater at Location 2 than at Location 6. Most of the observed spatial variation in phytoplankton

-) probably resulted from the combined effects of differences in water depth and introductions of river populations near Location 2. As the cooling lake A filled during the year, spatial differences became less pronounced.

for several reasons, the data from Location 6 was considered a more accurate representation of cooling lake phytoplankton in 1981.3-17

4. PER IPHYTON 4.1 FIELD AND ANALYTICAL PROCEDURES Periphyton was scheduled to be sampled from plexiglass artificial substrates in the Neosho River in April, June, October, and December 1981. Fluctuating river flows caused siltation and immersion problems and loss of platforms.

Periphyton was sampled at Locations 10 and 4 in the Neosho River on 27 April 1981. Only Location 4 was sampled on 29 June because of siltation problems with the substrates at Location 10. No August -ollections were made because a sharp drop in river flow resulted in improper immersion of the substrates at both sites. On the remaining two sampling dates, 29 September and 19 October, only Location 10 was sampled due to the loss of the substrate platform at Location 4 during September.

Periphytic algae were allowed to colonize and grow on the artificial substrates for 27 days prior to collection for the first three collections and 20 days for the October collection.

On each sampling date, duplicate samples were collected for itentification, enumeration, and biovolume determination and preserved with M (Meyer 1971). Four replicate samples were taken for biomass and chlorophyll a measurements.

These were stored on ice under dark conditions until further processing.

Periphytic algae were identified to the lowest positive taxon and enumerated.

Diatoms were cleaned chemically (Hohn and Hellerman 1963) and analyzed from Hyrax mounts under oil immersion at 1250X magnification.

Nondiatoms were analyzed from wet mounts at 50OX magnification.

Taxonomic references used for identifications were similar to those used Tor the phytoplankton (Section 3.1). Algal abundjnce was expressed as the number of units per square millimeter (No./mm ) using reporting units similar to those used for phytoplankton except for filamentous algae which were reported in lO-um lengths. Biovolume was determined by methods descri)ed for phytoplankton and expressed as microliters per square decimeter (pl/dm ). Shannon's (1948)diversity index and evenness indices (Zar 1968) were calculated using base 2 logarithms.

Biomass was used at each location as an index of the organic content of the periphyton community, and chlorophyll a concentrations provided an index of the algal component.

Biomass (ash-free dry weight, gravimetric method) and chlorophyll a (corrected for pheophytin, spectrophotometric method) were measured in accordance with Standard Methods for the Examination of Water and Wastewater (APHA 1976). Production rates were reported as milligrams per square decimeter per day (mg/din /day) and micrograms per square decimeter per day (pg/dm /day) for biomass and chlorophyll a, respectively.

4.2 RESULTS AND DISCUSSION During 1981 one hundred sixteen taxa were identified from periphyton samples collected in the Neosho River near WCGS (Table 4-1). Diatoms (Bacillariophyta) were the most abundant algal group, making up more than 80 percent of the total density and biovolume of the algal assemblages on all 4-I I!1 Fl[)9 6 0 6 TABLE 4-1 PERIPHYTIC ALGAE IDENTIFIED IN THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1981 BACILLARIOPHYTA Centrales C~clotella atomus Hustedt Cycloteila lutzintana Thwaltes Cyclotella meneghiniana Kuetzlng Welosira a (runow) 0. Mueller Ml-Osira di-tans (Ehrenberg)

Kuetzing Melosira distans var. l1rata (Ehrenberg)

Bethge MelostraT ranyta (Eh-renbeg)

Ralfs Melosira varlns 'C.A. Agardh Skeletonema

-otamos (Weber) Hasle Stehanodiscus astraea var. minutula Grunow Ste anodscus Tnvistatus Hohn and Mellerman Ste hanod scus spp.Penna les Achnanthes exl ua Grunow Achnanthes Taneolata (Orebisson)

Grunow Achnanthes lanceolata var. dubia Grunow Achnanthes linears (4W. SmifthT-runow Achnanthes mTnuttssfma Kuetzing rJUsllla Grunow r veneta Kuetz ng Ca one s bac llum (Grunow) Cleve C nes pe auus Ehrenberg Cocconeis a Ehrenberg bela se e Rabenhorst Ujefla p t var. auerswaldii (Rabenhorst)

Reim Se lnuata Gregory nticula .eg Kuetzlng lD s aP(Schumann)

Cleve ap-erlae (Kuetzing)

Petersen ,1 anema ivace (Lyngbye)

KuetzlngýG o T (Kuetzi ng) Kuetzing honm subca atum var. mexicanum (Grunow) Patrici P 0.oroiqaotus-at-Ui su111van and Wormley) Boyer ay cula iarvensis Hustedt Navicu a aur cul-ta Hustedt Navicula ca itata var. hungarica (Grunow) Ross Navicula ryptocehala Kuetzing Navicula ryptocehaa var.veneta (Kuetzing)

Rabenhor Navicura ITodesA. Mayer avicula heufleri var. leptocephala (Brebisson ex Grunow) Patrick Navicula menisculus var. upsallensis (Grunow) Grunow Navicua minima Grunow avicu a Mnuscula Grunow Navicula i-nus-cul odes Hustedt avicula pelliculosa (Brebisson ex Kuetzing)

Hilse Navicula Wu Kuetzin9 avcua rhynchoce hala Kuetzing Navicua rhynchocehaa var. germainii (Wallace)

Patr Navicuwa tl Patrick NavTc-ula tenera Hustedt ulath-ienmannl I Hustedt Navicua Etr nctata var. schizonemoides (Van Heurck)Patrick Navicula utermoehllt Hustedt Navicula vaucheriae Petersen Navicula vitabunda Hustedt Navicula spp.Nitzsch'a acicularis (Kuetzing)

W. Smith Nitzschia acicularis var. adelos Kohn and Hellerman Nitz-sc7a -am arunow N(tzschlaa (Gregory)

Grunow tHitzschig ca da Giunow BACILLARIOPHYTA (continued)

Nitzschta dissipjta (Kuetzing),Grunow Nitzschia filiformis (W. Smith) Schutt it-zschta font cola Grunow Nitzschia frustulum var. perminuta Hustedt NtIz sIcha h a~rca Grunaw Ntshakutzi--iana Hilse N1tzschla linears Agardh) W. Smith ftz-schta microcehala Grunow t S ea uetzing) W. Smith N..tzsihla raMana Grunow NItzschia

-,b- itellata Hustedt titzschta tryblaonell var. s (Arnott) A. Mayer Nltzscht spp.Pleurosmma delicatulum W. Smith Rhoicospenia curata 1Kuetzing)

Grunow ex Rabenhorst ar l austa Kuet~zng Surirclla suectca Grunow CHLOROPHYTA Ankistrodesmus Tfalcatus (Corda) Ralfs Chl amydomonas spp.C hloroon um elonfatum Dangeard CsaumSpp*i erilo ulchellum Wood lirchneriei'a subsolitaria G.S. West er Micractinum pusillmFresentus oacstis bo Snow Oa Hansglrg Pediastrum Fu-pex Meyen Pediastrum tetras (Ehrenberg)

Ralfsua lacustris jChodat) G.M. Smith Scenedesmus k a Turpin) Lagerheim k Scenedesmus dimor us (Turpin) Kuetztng Scenedesmus

_uadri-cauda (Turpin) Brebisson Schroederia set era chroeder)

J.Lmermann Selenastrum mnutium (Naegeli)

Collins Steocloni tenue C.A. Agardh) Kuetzing Ulathrix subtiltsstma Rabenhorst st UnIdentified green coccoid CRYPTDPHYTA C&y a Sspp.Khodomnas spp.CYANOPHYTA Anabaena spp.Chroacoccus dispersus (Keissler)

Lemmermann Chroococcus spp.Ic drquetlt Gomont in Harlot Ick LYn v mne Le rmann Ynqbva versi =oor (Wartmann)

Gomont Lynq.b. spp.Mertsmopedia tenulssima Lemnmermann Mieorys incerta Leumermann 0scT1i torta'11 ica Lemmermann Oscillatoria spp.Phormidiun tenue (Meneghinl)

Gomont UnidentfTeT7 Bue-g reen coocoid EUGLENOPHYTA Erea, aciis K1 ebs us cum7 atus Stokes P-w'-cus spp.

omonas spp.4-2 four sampling dates (Table 4-2). Blue-green algae (Cyanophyta) and green algae (Chlorophyta) were always present in low numbers, while euglenoids (Euglenophyta) and cryptophytes (Cryptophyta) occurred sporadically and always in very low numbers. Detailed information on periphyton densities, biovolumes, and relative abundance is presented in Appendix C.Periphyton densities and biovolumes were highest in 2 April at both Locations 4 and 10. Tojal density at Location 10 was 10,694/mm while totai biovolume was 18.79 Vl/dm .Locatio2 4 had a higher total density (11 ,437/m ) but a lower biovolume (13.61 lil/dm ). This resulted from differences in the major taxa (those composing at least five percent of the total density or biovolume; Table 4-3). Small taxa, such as Achnanthes lanceolata, Achnanthes minutissima, and Skeletonema Rotamos, were more abundant at Location 4, while somewhat larger forms, such as Cocconeis pediculus and Navicula tryptocehala, were more common at Location aO. The relative abundance of small centric diatoms at both locations resulted in part from upstream water releases from John Redmond Reservoir.

Several of the taxa at both locations were typical of those found in rivers and streams (Lowe 1974; Patrick 1948; Round 1973), particularly alkaline streams (Table 2-3; Hynes 1972).Chlorophyll a concentration, an index of algal production on the artificial substrates, was lower at Location 4 than at Location 10 in April (Table 4-4)because the former had more small taxa which contain lower amounts of chlorophyll

a. Biomass production, however, was higher at Location 4 than 10.Greater production apparently was due to the nonalgal component

<e.g., bacteria, protozoa, and other microorganisms) of periphyton at Location 4, as shown by differences in the Autotrophic Index at Location 4 (451) and at Location 10 (233). Considerable nonalgal production was actually occurring at both locations because values greater than 100 generally indicate that peri phyton is dominated by heterotrophic ( nonphotosynthetic) forms (Weber 1973).Only Location 4 was sampled in June. Total density was 36 percent of that in April, and biovolume was less than half (Table 4-2). Pennate diatoms were the dominant group (Appendix C), and species of Navicula and Nitzschla were common (Table 4-3). Biomass and chlorophyll a concentrations also were much lower, as was the Autotrophic Index (Table 4-4) which indicated that considerable nonalgal production was still occurring.

Lower densities and production in June probably resulted from greatly increased flow in the river (Table 2-2), a seven-fold increase in turbidity (Table 2-3), and increased shading caused by seasonal canopy development.

Only Location 10 was sampled in September because of the loss of the artificial substrates at Location 4. Small Achnanthes species (A. exigua, A.lanceolata, and A. minutissima) were the most important taxa numerically, while Cocconeis placentula was the most important taxon in terms of biovolume, making up 2B.5 percent of the total (Table 4-3). Biomass production was approximately half that of April, while the mean chlorophyll a concentration was more than twice as high (Table 4-4). The Autotrophic Index was quite low (39) indicating that most of the production occurring on the artificial substrates was algal.4-3 9 I.(.J ('1 6 TABLE 4-2 MFAN DENSITY, BIOHIIE AND PERCENT MON OF MKIX TAXNC1IC MLPS IN PERIPHYTN S*RB W..LECIED FRCM ARTIFICIAL SWSWTES IN 1*E NE(Jf RIOR NEAR WLF EEK IGEERATING STATION, BURI. SAS, 1981 Density at Sailing Locatiors Blovolune at Sawpling Locatioms 10 4 10 bypsure Period Taxon% lmd/n 4 1 AR -27 APR 26 MAY -22 JJN 3 SEP -29 SEP 30 SP -19 OCT Chlorofytta Cyano"iyt Eugl eota Total Peri fiittn Bacill arloiftyta Chlorofty'U Cy~a" EuglenoftU Total Per!fttf Bacillarioiy:ta Chlorojmiyt C yanopVt Eug1 sowta Total Perifttyton Baclla-loiyftU Chlorofytta Cyano" Euglenopyta Total Peri Oiton 8,677 712 48 3 10,M4-(a)0 315 3 8,711 208 7 0 31 1 247 81.1 6.7 0.4 11.7<0.1 100.0 10,154 817 19 358 0 11,347 3,436 77 0 580 0 4,092 89.5 7.2 0.2 3.2 0 100.0 84.0 1.9 0 14.2 0 100.0 15.59 2.12 0.2D 0.49 0.3B 18.79 83.0 11.3 1.1 2.'6 2.0 100.0 11.54 1.80 O.OB 0.19 0 13.'61 5.17 0.15 0 0.31 0 5.57 84.8 13.2 0.'6 1.4 0 100.0 93.0 2.7 0 4.4 0 100.0 94.9 1.4 0 3.6 0.1 100.0 84.2 2.8 0 12.7 0.3 100.0 12.64 0.38 0 0.11 0.03 13.17 0.93 0J2 0 0.02 O.C2 0.99 96.0 2.9 0 0.8 0.3 100.0 93.5.2.0 0 2.4 2.1 100.0 o o ew (a) Nort ed.4-4 TABLE 4-3 ALGAL TAXA CIMPOSING AT LEASW 5 PERCENT OF IOTAL DENSITY OR BIOALUJME OF PERIIY C IJECIED FRO4 ARTIFICIAL SIBSTRAlES IN THE NEO RIVER NEAR WCLF aME STATI{N, BaRLINGIVN, KANSAS, 1981 I.ci]Location 10 Density Biovol uneýNojn?). (iil/dn 2)Location 4 Density Bi vol wne tu2d~Exposur Period and Taxa 01 APR -27 APR anthe laneolata AcNanthes miuitissina Coccoeis pDaioulus Cyclotella atams Cyclotel la k ngiana It a mnein ana a %cFAWtoojA31a Skeletorema t Stel*Wi scus astraea var. minuitula Stigeoclaniuu tenue 26 MAY -22 JUN Caloneis bad11un Melosira varians Navicula graciloides Navicula menisculus va. upsaliensis W1 caud i riae N~itzsd-ia dissipat~Nitzachis frustulun var. imita 03 SEP -29 SEP 70ýý!ýata Wanthe nmiutissima Cocconeis Ilacentula WIWI menisculus var. upsaliensis Navicula miusla Navicula tir vat. shimzor ides 0 247 190 1,832*1 ,096*1 ,25*437 342 767" 329.a)766*2,S30*56D*72Dk 872*554*213 0 343*3.40* 0.48 3.23*0.32 2.19*0.10 1.04*0.78 989*g1*32 1,914*539 1,794*160 326 159 76 9 18D ,291*68,2*0.81*0.22 0.57 0.61 0.24 4.61" 0.05 1.11*0.47 0.86*0.77*0.10*0.38t 0.28*0.73*0.65*0.07 0.36*0.103 0.55 1.82*0.36 3.75*1.31" 0.25 0.83*0.02 0.163*40.01 0.09*4 30 SEP -19 OCT Adhnanthes lanMlata 20*Coccorels placitula 134*Lyncbya pp.26*.. astraea var. minutula 10 (a) N m srpled.Nate: Asterisk (*) designates a value that was at least 4-5 4 5 pement of the total.

F)F)TABLE 4-4 MEAN BIOMASS AND CHLOROPHYLL a PRODUCTION, AUTOTROPHIC INDEX, NUMBER OF TAXA, AND DIVERSITY-AND EVENNESS MEASURES FOR PERIPHYTON COLLECTED FROM ARTIFICIAL SUBSTRATES IN THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1981 -Parameter Biomass 2 production(a)(mg/dm per day)Chlorophyll a production(c)(v9/gm/ per day)Exposure Period Sampling Location 10 4 Autotrophic Index(d)01 26 03 30 01 26 03 30 01 26 03 30 01 26 03 30 01 26 03 30 01 26 03 30 APR MAY SEP SEP APR MAY SE P SE P APR MAY SEP SEP APR MAY SEP SEP APR MAY SEP SEP APR MAY SEP SEP 27 22 29 19 27 22.29 19 27 22 29 19 27 22 29 19 27 22 29 19 27 22 29 19 APR JUN SEP OCT APR JUN SEP OCT APR JUN SEP OCT APR JUN SEP OCT APR JUN SEP OCT APR JUN SE P OCT 0.68 0.14 8.16 19.20 1.70 Number of Taxa(e)233 39 91"64 44 52 1 .45 3.5S 0.75 451 331 55 53 4.13 4.564 0.71 0.81 Di versity~f) 4.43 3.77 2.99 0.74 0.t69 0.'53 Evenness(g)(a)(b)(c)(d)(e)(f)(9)Ash-free dry weight.Not sampled.Corrected for pheophytin.

Weber 1973.Total taxa of two replicates.

Shannon 1948, using base two logarithms.

Zar 1968.4-6 I N E: In October, total density and biovolune at Location 10 were only a fraction of that occurring during previously sampled months. Biomass and chlorophyll a levels were also lower. This resulted in part from a seven-day shorter A) exposure time for the artificial substrates.

Also, some nutrients such as 6; phosphorus and silica, were present in lower concentrations (Table 2-5). Most of the production in October was algal, based on the Autotrophic Index 191).Diversity values at Location 10 decreased through the 1981 sampling period (Table 4-4). Diversity in April was 4.43 and then dropped gradually to 2.99 In October. Location 4, which was sampled only twice, had diversities of 4.13 in April and 4.64 in June. Evenness values followed a pattern similar to that for the diversity.

All diversity and evenness values reported during 1981 were within the range of values reported in 1980 for periphyton communities on artificial substrates with the exception of the low October values {Ecological Analysts 1981).Many of the same periphytic taxa were observed during 1981 and 1980 (Ecological Analysts 1981), and diatoms were always the most abundant algal group during both years. However, fewer taxa were identified in 1981. Green algae and blue-green algae were less numerous in 1981, and chrysophytes, which were present in low numbers in June and August 1980, were not observed in 1981. Differences in major taxa were also noted between the two years.4.3 SUM4ARY AND CONCLUSIONS Many of the periphytic taxa observed during 1981 in the Neosho River near the Wolf Creek Generating Station were typical of those found in alkaline rivers and streams. John Redmond Reservoir, which is upstream from the sampling locations, also influenced the periphyton composition by contributing some of the small centric diatoms which were observed.

Many of these taxa were observed during past studies (Hazleton Environmental Sciences 1980; Ecological Analysts 1981), but densities, biovolumes, and composition of the major taxa differed.

Problems with the artificial substrates precluded most comparisons between the sampling locations and conclusions about seasonality of the per phyton.Hydrological conditions in the Neosho River related to variable water releases from John Redmond Reservoir has hampered the collection of periphyton samples from both natural (1973-1979) and artificial substrates (1980-1981) during environmental studies for WCGS. Periphyton has been eliminated through scouring, siltation, or desiccation depending on the flow conditions which preceded scheduled collections.

Retrieved samples have provided an extensive qualitative data base for periphyton community composition and productivity but quantitative temporal and spatial information has been limited because of missed samples. Further attempts to provide this data are not felt to be warranted at this time.4-7 ri A F V.5. ZOOPLANKTON 5.1 FIELD AND ANALYTICAL PROCEDURES u Duplicate zooplankton samples were collected quarterly from the Neosho River o) at Locations 1, 10, and 4 (Figure 1-1). Each replicate consisted of a , stationary horizontal surface sample of 0.5 to 3.0 minutes duration with a metered No. 25 mesh (64-pm aperture) plankton net with a 437-cm4 aperture.When flow was minimal or absent, the net was towed at a speed sufficient to provide reliable flowmeter readings.

The zooplankton community in the cooling lake was sampled bimonthly with bottom to surface tows utilizing a 30-cm diameter No. 25 (65-pm) mesh conical plankton net. Each sample was placed in an appropriately labeled glass jar containing menthol crystals to relax the body structure of rotifers which facilitated identification.

Within one hour of collection, samples were preserved with 5 percent formalin, then transported to the laboratory for analysis.Samples were concentrated or diluted in the laboratory to a workable density of organisms

(=lO0-200/ml), and thoroughly mixed to obtain a representative subsample, which was withdrawn and placed in a Bogorov counting chamber.Stratified counts of zooplankton in the subsample were made using a binocular dissecting microscope at lO-70X magnification.

Difficult specimens were identified at 100-IOOOX with a compound microscope.

All organisms were counted in the first two 'subsamples.

As progressively larger aliquots were withdrawn, abundant taxa were eliminated from consideration.

Subsampling was continued until a sufficient number of organisms was enumerated to estimate population densities, usually after counting at least 5 percent of the total sample. Subsequent to quantitative analysis of cooling lake zooplankton samples, additional aliquots were examined qualitatively to identify addi-tional taxa. Microcrustacea were identified to species with the exception of taxonomically indistinct immature copepods and cladocerans which were identi-fied to the lowest positive taxon. Rotifers were identified to genus, except certain littoral benthic genera in the order Bdelloidea.

Identifications were made using appropriate taxonomic keys, including Brooks (1957, 1959), Wilson and Yeatman (1959), Ruttner-Kolisko (1972), Smirnov (1974), and Pennak (1978).To determine wet, dry, and ash-free zooplankton biomass standing crop esti-mates in the cooling lake, four bottom to surface tows were collected at each location and placed on ice for transport to the laboratory.

Each replicate was filtered by suction and blotted on paper towelling.

Samples were then weighed to the nearest 0.1 milligram on a Mettler HIOT balance to obtain wet weight estimates.

Samples were then washed into a crucible and dried at 60 C for 24 hours2.777778e-4 days 0.00667 hours 3.968254e-5 weeks 9.132e-6 months to obtain dry weight values (Lovegrove 1962). Ash-free weights were determined by incinerating the sample n a muffle furnace at 500 C for 1 hour1.157407e-5 days 2.777778e-4 hours 1.653439e-6 weeks 3.805e-7 months . Each parameter was expressed as mg/mn of water sampled.5.2 RESULTS AND DISCUSSION 5.2.1 Neosho River Zooplankton populations in the Neosho River were dominated by rotifers, primarily by the rotifer genera Brachionus, Keratella, Polyarthra, and 5-1

]E.D4 Synchaeta (Table 5-1). These taxa have been dominant groups in the river since studies began in 1973 and are among the most frequently encountered rotifers in other midwestern rivers as well as throughout the United States (Williams 1966). Copepods, primarily immature forms, were numerically important throughout the year. Cyclops vernalls and Dlapomus siciloldes C) were the most common adult copepods identifiTd; however, both exhibited low mean densities (generally

<2,500 organisms/rn).

D. stciloides has been the most common adult copepod in the river since 1973"while densities of C.vernalis have varied annually.

Cladocerans, basically immature daphnTds, were insgnificant contributors to the mean total river population and never comprised more than 1.6 percent of the mean total zooplankton (Table 5-2).The remaining 48 taxa identified from the Neosho River (Table 5-3) generally comprised (1.0 percent of the total zooplankton standing crop (Appendix D).Mean total zooplankton density in the tailwaters of John Redmond Reservoir (Location

1) ranged from 42,018 organisms/m0 in April to 234,312 organ-isms/m 3 in October (Table 5-1). The zooplankton community In April, June, and October was dominated primarily by copepod nauplit and Keratella which, when combined, represented 81.6, 71.6, and 87.8 percent of the total zoo-plankton, respectively.

Populations of copepod nauplii peaked at .2,208 organisms/mnr in June while Keratella peaked at 177,308 organisms/mor in October. Maximum densities of the rotifers Synchaeta (49,966 organisms/m 3)and Polyarthra (11,408 organisms/in

3) at Location 1 occurred in December and when combined with copepod nauplil and Keratella accounted for 96.9 percent of the mean total zooplankton.

Annual microcrustacean density at Location 1 declined 35 percent from 1980 to 1981 (Table 5-4). This decline, which began in 1980, affected most micro-crustacean taxa. The reasons for reduced microcrustacean -density are obscure, but complex biotic and abiotic factors influencing John Redmond Reservoir may result in changes in zooplankton abundance.

Year to year differences in reservoir water exchange rates (Brook and Woodward 1956, Tonolli 1961), 4 predator-prey relationships between zooplankton and young of the year and juvenile fish (Johnson 1964, Thayer et al. 1974) as well as the influence of zooplankton recruitment from the discharges of upstream reservoirs (Tonolli 1955, Cowell 1970, Martin and Novotny 1977) may create annual variation in zooplankton standing crops. Dominant fish species in John Redmond Reservoir are known to feed selectively on Cyclops, Bosmina, and Dajphnia in other midwest waters (Kutkuhn 1957, Cramer and Marzolf 1970) and the feeding preference for these dominant microcrustacean groups in John Redmond may partially account for the reduced microcrustacean densities observed at Location 1 during the past two years.Zooplankton density and composition at downstream Locations 10 and 4 generally reflected the seasonal zooplankton trends at Location 1. During April, zooplankton populations at Locations 10 and 4 were dominated primarily by the rotifers Keratella, Brachionus, Synchaeta, and Polyarthra (Table 5-1).These four taxa accounted for 91.2 and 93.7 percent of the total zooplankton at Locations 10 and 4, respectively (Table 5-2). The relationship of Neosho River flows to the contribution and movement of reservoir zooplankton to the downstream locations was apparent.

Low river flow in April (29 cfs) resulted 5-2 TABLE 5-1 MEAN DENSITY (No./m 3)(a)OFCODOMINA!ITZOOPLANKTON IDENTIFIED FROM SAMPLES COLLECTED IN THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, APRIL THROUGH DECEMBER 1981 22 JUN I___ in 4 20 OCT 1 10 4 Taxa Copepoda Nauplil Calanold copepodites Cyclopold copepodites 9 2 vernalis ajtusiTE 1iodes Cladocera Daphnia spp.(immature)

Rotifera Odellold rotifers Brachionus spp.Kraela spp.vPolvarthra spp.Synchaeta spp.Total Zooplankton CI 17.951 12,065 438 1,708 2,286 1,403 546 286 23,521 32 946 23,352 165 0 284 195 0 72 8 4 99 0 29.589 1,093 7,067 12,532 1,982 5,745 1,210 980 3 206 13 6 489 0 40,028 1,336 11,384 11,512 6,665 7,962 44,700 32.208 4,636 3,709 1.378 2.501 1,428 696 41,479 49 6,295 30,500 1,318 98 20,528 16,261 1,732 1,250 206 1,021 554 195 25.104 160 4,416 17,866 906 276 17,832 14,098 1,244 1,168 244 1,065 434 166 18,640 231 4.939 11,404 590 193 36,125 28,337 1,114 4,026 806 1,478 2,350 1,834 195,837 0 3,096 177,308 1,238 12,764 11,278 8,274 332 1.458 442 662 1,047 807 64,425 0 596 58,289 530 3,728 12,959 9.977 342 1,562 344 488 640 521 77.460 344 1,617 66.462 570 7,076 15 EC 1 10 4 12,111 11,163 13,343 8,944 8,840 10,860 54 114 111 2.865 2,011 2,140 182 112 137 26 40 43 408 396 484 274 250 282 112.174 102,544 125,202 0 0 152 80 34 51 50.442 46,036 57,855 11,408 9,010 9,846 49,966 47,396 56,992 124.693 114,103 139,029 42,018 29,972 41.727 87,607 46,186 36,906 234,312 76,750 91,059 ('a) Mea of two replicates.

TABLE 5-2 PERCENT COMPOSITION OF DOMINANT ZOOPLANKTON IDENTIFIED FROM SAMPLES COLLECTED IN THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, APRIL THROUGH DECEMBER 1981 Taxa Copepoda Nauplii Calanold copepodites Cyclopoid copepodites Cyclops vernalis Diaotomus siciloldes Cl adocera Daphnia spp. (immature) 27 APR 1 10 4 22 JUN 1 10 4 20 OCT 1 10 4 42.7 28.7 1.0 4.0 5.4 3.3 0.9 0.6 0 0.2<0.1<0.1 2.9 2.4<0.1 0.5 (0.1 0. 1 51.0 36.8 5.3 4.2 1.6 2.9 44.4 35.2 3.8 2.7 0.4 2.2 48.3 38.2 3.4 3.2 0.7 2.9 15.4 12.1 0.5 1.7 0.3 0.6 14.7 10.8 0.4 1.9 0.6 0.9 14.2 11.0 0.4 1.7 0.4 0.5 15 DEC 9.7 9.8 9.6 7.2 7.7 7.8<0.1 0.1 0.1 2.3 1.8 1.5 0.1 0.1 0.1<0.1 <0.1 <0.1 1.3 0.3 1.2 1.6 1.2 1.2 1.0 1.4 0.7 0.3 0.3 0.3 0.7 0 0 0.8 0.4 0.4 0.8 1.1 0.6 0.2 0.2 0.2 Ut Rotifera Bdellold rotifers Brachionus spp.Kerate=-a spp.Polyrtbra spp.Syrchaeta spp.56.0 0.1 2.2 53.2 0.4 0 98.7 3.6 23.6 41.8 6.6 19.2 95.9 3.3 28.4 28.8 16.6 19.9 47.3 0.1 7.2 34.8 1.5 0.1 54.4 0.3 9.6 38.7 2.0 0.6 50.5 0.6 13.4 30.9 1.6 0.5 83.6 0 1.3 75.7 0.5 5.4 83.9 0 0.8 75.9 0.7 4.9 85.1 0.4 1.8 72.9 0.6 7.8 90.0 0 0.1 40.5 9.1 40.1 89.9 0 (0.1 40.3 7.9 41.5 90.1 0.1<0.1 41.6 7.1 41.0 ft m 41b a ITABLE 5-3 QUALITATIVE DISTRIBUTION OF ZOOPLANKTON TAXA IN THE NEOSHO RIVER AND COOLING LAKE (LOCATIONS 2, 6) NEAR WOLF CREEK ýGENERATING STATION, KANSAS, FEBRUARY THROUGH DECEMBER 1981 Neosho River Cooling Lake.COPEPODA Naupl ii X X X Calanold copepodites X X X Cyclopoid copepodites X X X Cyclops btcuspidatus thomasi Forbes X X X Cyclops vari cans rubellus Ltlljeborg X X X Cyclops vernalls Fischer X X X Diaptomus claviipes Schacht X X X Diaptomus pallidus Herrick X X X Diaptomus siciloides Lilljeborg X X X Co E agilis (Koch) X X Ergasilus sp. Nordmann X -X Ergasilus chautauquaensls Fellows X X X Harpacticoid copepods X X Mesocycl os edax (Forbes) X X X Tro cyclops Drasinus (Fischer)

X X CLADOCERA Alona spp. Baird X MTna circumfimbriata Megard X X X Bosmina iongirostris (O.F. Muller) X X K Ceriodaphnia spp. (immature)

X X Ceriodapho a ouadrangula (O.F. Muller) X X x Ceriodaphnia rlgaudi Richard X Ceriodaphnla reticulata (Jurine) X X Ch(dorus sphaercus

!O.F. Muller) X X X Daphnia spp. (immature)

X- X X Daphnia ambigua Scourfield X X Daphnia galeata mendotae Birge x D a parvula Fordyce X X X Daphnia pulex Leydig X X Daphnia retrocurva Forbes X X X Diaphanosoma brachyurum (Lieven) X Diaphanosoma leuchtenbergianum Fischer X X K Disparalona rostrata (Koch) X Ilyocryptus sordidus (Lieven) X Kurzia latissima (Kurz) X Leptodora kindtii (Focke) X X X Leydigia acanthocercoides (Fischer)

X Leydigia leydigi (Schoedler)

X X Macrothrix laticornis (Jurine) X X Moina micrura Kurz X X Moina minuta Hansen X woTi 7ieirizeiskii Richard X P-Teuroxus denticulatus Birge X Pleuroxus hamulatus Birge X X Simocephalus sp. Schodler X 5-5 E: I TABLE 5-3 4CONT.)Neosho River Cooling Lake"' 2 6 0 CLADOCERA (CONT.)0; ~ Simocephalus eexpnosus (Koch) X Simocehalu Eerruatus (Koch) X Simocephalus vetulus (O.F. Muller) X ROTIFERA Anuraeopsis sp. Lauterborn X Asplanchna spp. Gosse X X X Bdelloid rotifers X X Brachionus spp. Pallas X X X Cephalodella spp. Bory St. Vincent X X Chromogaster sp. Lauterborn X Collotheca sp. Harring X X X Colurella sp. Bory St. Vincent X Conochilotdes sp. Hlava K X Conochilus sp. Hlava X X Filinla spp. Bory St. Vincent X X X Hexarthra spp. Schmarda X X X Kelltcottia spp. AhIstrom X X Keratella spp. Bory St. Vincent X X X Lepadella spp. Bory St. Vincent X X x Lophocharis sp. Ehrenberg X Monosta spp. Ehrenberg X X Mytilina sp. Bory St. Vincent X X at §as sp. Harring K Ploesoma sp. Herrick X PolYarthra spp. Ehrenberg X x X Pompholyx sp. Gosse X X Rotaria sp. Scopoli X Synchaeta spp. Ehrenberg X x X Testudinella sp. Bory St. Vincent K Trichocerca spp. Lamarck X X X Trichotria sp. Bory St. Vincent X Tripleuchlanis sp. Myers X 5-6 I in statistically different microcrustacean densities among locations

<F 2 3) =22.82; P < 0.05) and a mean 48-fold decrease in density from Location 1 to Location 10. The decrease in abundance was evidenced by the apparent loss of limnetic reservoir taxa present at Location 1 such as Bosmina longirostris, immature daphnids, Daphnia parvula, Diaptomus siciloides, and Cyclops vernalis Fi (Table 5-1). Low river flow apparently prevents effective downstream movement of reservoir zooplankton taxa and downstream populations may be characterized as remnant reservoir populations augmented with taxa resulting from autoch-thonous production.

Autochthonous production has been noted at Locations 4 and 10 in previous studies of the Neosho River (Repsys 1978, 1979b, Rogers 1980, Ecological Analysts 1981). There was a subsequent 4-fold increase in microcrustacean density between Locations 10 and 4 in April, which was due to apparent production of littoral cladoceran taxa such as the Alonids and Chydorus sphaericus and the rotifers Brachionus, Polyarthra, Synchaeta and Bdelloid rotifers.

Microcrustacean production between the downstream Loca-tions 10 and 4 has been most apparent during low river flow and has been related to habitat differences among locations.

During each sampling period dominant taxa were similar between locations although zooplankton abundance generally decreased downstream (Table S-1).The most dramatic reduction usually occurred during periods of low water releases from John Redmond Reservoir.

These downstream decreases have been observed during past Neosho River studies and have been documented by Ward (1975) and Armitage and Capper (1976) for river-reservoir systems in Colorado and Wales. Reservoir discharge (cfs) and annual microcrustacean density was significantly correlated at Locations 10 (r = +.9330; P ( 0.05)and 4 (r = +.9328; P < 0.05) suggesting that as flow increased more organisms were transported downstream.

Conversely, when a low flow regime exists, reservoir zooplankton decrease in abundance downstream and apparent autoch-thonous zooplankton production increases.

During June, immature copepods and the rotifers Keratella and Brachionus together composed 83.5 and 82.5 percent of the total zooplankton at Locations 10 and 4, respectively.

High river flow (2,367 cfs) resulted in no signifi-cant differences in total microcrustacean density among locations (F 2 , 3 = 7.08;P > 0.05); however, all major taxa decreased In abundance at the downstream locations.

Mean total zooplankton at downstream locations increased 10-fold from June to October and reached maximum annual densities in December (Table 5-1).immature copepods and Keratella comprised the majority of zooplankton in October while Synchaeta combined with the former two groups to represent 98 percent of total zooplankton in December (Table 5-2). There was no signifi-cant difference in total microcrustacean density among locations in December (F2i = 1.46; P > 0.05) which corresponded to relatively high river flow (1,620 cfs).Construction of the Wolf Creek Generating Station has had no apparent effect on the zooplankton community in the Neosho River. Zooplankton species composition closely resembled that present during previous years. Although mean annual microcrustacean densities declined during the past two years, the reductions were related to factors independent of construction activities.

'5- 7 rr~ ,7\.r\K , -. r C~ 1' 1 -. N 11-s rr,,-~ .,~ ~TABLE 5-4 ANNUAL MEAN DENSITIES (No./m 3) OF SELECTED MAJOR RESERVOIR (LOCATION 1). 1973 THROUGH 1981 MICROCRUSTACEAN TAXA FROM JOHN REDMOND lear Taxa Copepoda Calanold (Diaptomus) copepodites Cyclopoid copepodites yclops vernalis DIapt omus pallidus Diaptomus siciloides All adult Diaptomus spp.Ergasilus chauta~uuaensis Total Copepoda(b)Cladocera 00 Bosmina lon irostris Cer, dodaphnia spp.(c)Daphnta parvula All Daphia spp.(d)(juveniles and adults)Dtaphanoso~a leuchtenbergianum Motna spp.(e)To-al Cladocera(b)

Total microcrustaceans(b) 1973 1974 2,635 6,948 2,546 6,558 284 597 17(a 1,006 1,418(a) 1,171 2,591 2,177 133 39 8,188 16,319 1975 .1976 8,963 9,006 443 48 3,458 3,508 756 22,676 10,580 130 2,870 3,994 8,418 7,484 30,943 1,698 13,182 976 316 1,014 1,330 282 17,468 20,980 11 3,547 3,579 3,344 259 28,173 1977 4,903 16,978 1,106 266 2,502 2,810 217 26,014 14,923 28 3,333 3,343 4,877 1,102 24,273 1978 1979 1980 1981 5,447 21,601 1,432 390 5,110 5,501 154 34,135 30,484 17 1,134 1,857 1,391 1,921 35,670 6,376 23,220 1,750 878 6,772 7,650 97 39,093 7,584 53 3,889 7,955 3,952 649 24,082 3,614 4,242 1,400 339 4,529 4,879 110 14,245 129 0 190 979 14 0 1,312 4,509 1,022 1,271 14,413 3,032 163 23,139 4,247 2,483 2,095 5,089 2,759 205 14,783 1,560 3,077 1,163 90 1,352 1,499 16 8,757 90 2 113 928 50 8 1,191 9,948 31,327 31,102 53,619 45,641 50,287 69,805 63,175 15,367 a Males only. Females not identified to species in 1973.b Includes the above selected taxa only.(c) Mainly C.C. ]acstris.(dN Mainly juveniles and adults of 0. parvula.(e Mainly M. micrura.a a a a A 5.2.2 Cooling Lake Sixty taxa representing 41 genera were identified from the -cooling lake during 1981 (Table 5-3). All taxa with the exception of Ceriodaphnia rigaudi and Daphnia retrocurva have been recently identified from John Redmond Reservoir anT the Neosho River. C. rigaudi has been reported in Kansas (Prophet and Waite 1974) although its distribution has generally been restricted to the southern United States (Brooks 1957; Pennak 1978). D.retrocurva has been identified in Kansas (C. Prophet, personal communication, Emporia State University, Emporia, Kansas) although it occurs seasonally and is never abundant.

Dominant taxa were similar between Locations 2 and -6;however, diversity was slightly greater at Location 2 (52 taxa) when compared to Location 6 (45 taxa). Location 2 represented a littoral habitat (=3 meters depth) conducive to zooplankton production and greater diversity (Hutchinson 1967), whereas Location 6 affords a limnetic habitat (=10-15 meters depth) less desirable for establishment of a diverse zooplankton assemblage.

Mean annual zooplankton density in tte cooling lake was 401,491 organisms/rn 3 with a range from 72,160 organisms/mn to 1,134,196 organisms/mw at Location 6 in August and February, respectively (Table 5-5). Rotlfers, primarily Keratella and Polyarthra and copepod nauplii combined to represent 89.3 percent of the annual zooplankton standing crop (Table 5-6). Excluding the February collection, annual total zooplankton density was almost 2-fold higher at Location 2 than at Location 6 due to apparent higher littoral zooplankton production.

Zooplankton population densities are usually high during initial impoundment and filling of new reservoirs.

Recently inundated areas favor the transport of suspended and dissolved materials into the reservoir ecosystem which promotes primary and secondary (zooplankton) production

<Rodhe 1264, Applegate and Mullen, 1967). The mean nnual copepod (148,526 organisms/&3) and cladoceran (7,026 organisms/mn) densities in the cooling lake fell within the range of densities reported for these groups in other midwestern reser-voirs. Mean annual rotifer densities in the cooling lake (annual mean of 245,946 organisms/m

3) appeared similar to or slightly lower than in other reservoirs.

The mean annual dry-weight biomass in the cooling lake was 155.8 mg/m 3 and ranged from 29.2 to 337.8 mg/m 3 at Location 6 in October and February, respectively (Table 5-7). Th( mean biomass at Locations 2 and 6 on comparable sampling dates was 156.6 mg/o3 and 110.7 mg/mr, respectively.

The difference in biomass between locations was reflected by the almost 2-fold higher zooplankton density at Location 2 than at Location 6. A correlation between total zooplankton abundance and dry weight biomass was evident<Figure 5-1) although the relationship was not statistically significant (r = +.754; P > 0.05). Biomass normally increases when zooplankton density increases (Cowell 1967). However, this trend was not consistently observed in the cooling lake. Disparity in the relationship between density and biomass indicates that the biomass estimates may be biased due to substantial amounts of phytoplankton that were encountered seasonally and retained with the No. 25 netting. The weight bias may be negated by use of a No. 10 5-9 I. fl- tltt-~TABLE 5-5 DENSITY (No./m 3) OF DOMINANT ZOOPLANKTON IDENTIFIED FROM SAMPLES COLLECTED IN THE COOLING LAKE NEAR WOLF CREEK GENERATING STATION, FEBRUARY THROUGH DECEMBER 1981 Taxa Copepoda Nauplil Calanold copepodites Cyclopold copepodites Diaptomus sicilotdes Cladocera Bosmina lon frostrJs a spp.(immature) inosoma spp.Rotifera a n spp.nch otdes spp.Keratella spp.LL spp.POMpol yx spp.SYnchaeta spp.23 FEB-214,420-163,488-3,301-22,794-15,248-943-629-0-0-918,833-8,803-91,176-144,624-669,672 0 629 , 28 APR 23 JUN , 25 AUG 19 OCT 16 DEC 2 6 2 6 2 6 2 6 2 6 132,989 84,888 1,792 22,636 6,885 1,509 1,132 283 0 233,159 943 0 226,368 2,830 0 2,358 85,376 66,560 2,880 11,008 3,328 6,976 192 3,712 0 960 0 0 896 0 0 64 508,013 386,630 28,917 67,896 19,487 8,110 1,132 4,149 472 104,984 25,145 1,259 35,202 8,803 10,689 627 85,529 60,352 4,715 11,316 4,526 34,609 15,371 11,882 472 28,291 472 15,842 4,244 3.583 0 0 49,785 36,000 6,650 4,655 1,700 6,180 60 240 5,080 57,902 7,182 1,330 4,655 18,800 0 11,039 59,720 42,400 12,000 1.080 2,480 5,520 0 2,080 2,400 6,920 560 0 2,040 2,800 440 160 124,537 84,044 4,081 17,264 11,928 6,593 0 3,767 942 408,918 4,944 1,412 275,438 29,662 9,888 81,925 540,048 51,509 38,784 269 6,128 2,626 3,232 0 741 741 290,476 0 0 149,480 46,460 11,312 81,608 345,217 160,710 142,727 1,072 12,624 476 3,334 834 1,310 0 375,554 0 0 146,776 153,862 0 74,906 539,588 114,510 95,982 786 11,651 1,867 2,162 344 393 0 289,248 0 0 144,624 102,966 0.40,872 405,920 Mean Total 2 6 195,207 101,844 146,858 77,928 8,502 3,992 25,015 10,663 8,095 5,012 5,145 8,907 632 2,756 1,950 3,135 1,299 614 236,103 255,788 7,643 1,639 800 17,836 137,688 74,318 42,791 137,580 4,115 1,959 34,171 25,556 436,453 366,536 Cu-.a Total Zooplankton

-1,134,196 367,657 93,296 Note: Densities based on a single monthly (a) Not collected.

621,107 148,429 113,867 72,160 sample from each location.Alb ak ah a a C rs .r, c:-s~ ~ -. I~' N -N ~-- flflr'n.-~-.,, TABLE 5-6 PERCENT COMPOSITION OF DOMINANT ZOOPLANKTON IDENTIFIED FROM SAMPLES COLLECTtO IN THE COOLING LAKE NEAR WOLF CREEK GENERATING STATION, FEBRUARY THROUGH DECEMBER 1981 23 FEB 28 APR 23 JUN 25 AUG 19 OCT 16 DEC Mean Taxa 2(a) 6 2 6" 2 6 2 6 2 6 2 6 2 6 Copepoda -18.9 36.2 91.5 81.8 57.6 43.7 82.8 23.1 14.9 29.8 28.2 44.7 27.8 Nauplii -14.4 23.1 71.3 62.2 40.7 31.6 58.8 15.6 11.2 26.4 23.6 33.6 21.3 Calanold copepodites

-0.3 0.5 3.1 4.7 3.2 5.8 16.6 0.8 0.1 0.2 0.2 1.9 1.1 Cyclopoid copepodites

-2.0 6.2 11.8 10.9 7.6 4.1 1.5 3.2 1.8 2.3 2.9 5.7 2.9 Dtaptomus siciloldes

-1.3 1.9 3.6 3.1 3.0 1.5 3.4 2.2 0.8 <0.1 0.5 1.9 1.4 Cladocera

-0.1 0.4 7.5 1.3 23.3 5.4 7.6 1.2 0.9 0.6 0.5 1.2 2.4 Bosmina longtrostris

-<0.1 0.3 0.2 0.2 10.4 0.1 0 0 0 0.2 0.1 0.1 0.8 DaiDhnia spp.(immature)

-0 0.1 4.0 0.7 8.0 0.2 2.9 0.7 0.2 0.2 0.1 0.4 0.9 Diaphanosoma spp. -0 0 0 0.1 0.3 4.5 3.3 0.2 0.2 0 0 0.3 0.2 Rotifera -81.0 63.4 1.0 16.9 19.1 50.8 9.6 75.7 84.1 69.6 71.3 54.1 69.8 BrachiQnus spp. -0.8 0.3 0 4.0 0.3 6.3 0.8 0.9 0 0 0 1.8 0.4 Cnoc~hlodes spp. -8.0 0 0 0.2 10.7 1.2 0 0.3 0 0 0 0.2 4.9 Kate spp. -12.7 61.6 1.0 5.7 2.9 4.1 2.8 51.0 43.3 27.2 35.6 31.5 20.4 jyarthra spp. -59.0 0.8 0 1.4 2.4 16.5 3.9 5.5 13.4 28.5 25.4 9.8 37.5 Piompbolyx spp. -0 0 0 1.7 0 0 0.6 1.8 3.3 0 0 0.9 0.5 Synchaeta spp. -<0.1 0.6 0.1 0.1 0 9.7 0.2 15.2 23.6 13.9 10.1 7.8 7.0 (a) Not collected.

ri p I'1 (I 6 TABLE 5-7 MEAN BIOMASS ESTIMATES(a)

COOLING LAKE (LOCATIONS 2 STATION. FEBRUARY THROUGH FOR ZOOPLANKTON COLLECTED IN THE AND 6) NEAR WOLF CREEK GENERATING DECEMBER.

1981 4: Date/Location Wet Weight 23 FEB 28 APR 23 JUN 25 AUG 19 OCT 16 DEC 2 (b)6 1,859.8 1,476.3 888.7 2 6 2(c)6(c)593.7 794.8 Dry Weight 337.8 227.6 142.8 92.4 100.5 205.2 153.1 86.4 29.2 171.4 127.9 Ash-free Weight 337.7 195.2 127.1'55.2 61.6 169.0 108.2'55.4.26.3 112.8 99.4 U 2 6 2 6 1,497.2 953.4 2(c)6 426.0 176.3 754.6 558.0 791.3 871.8 835.2 I Mean Annual 2 6 Mean 156.6 155.2 155.8 117.7 4 126.7 122.6 (a) Each parameter expressed in mg/m 3.(b) Not collected, location not inundated.(c) Data based on three replicates.

'5-12 (I E'l 12- 6 11 -Dret 1.0 5 9°E E0*-3-"I 2 C 5-4.- -23.8%/ " 2 " \ / "% % J I 1-I I I II FEB APR JUN AUG OCT DEC Figure 5.1. Comparison of total zooplankton density and dry weight estimate from the cooling lake for Wolf Crook Generating Station, 1981.5-13 (164-um) net which should exclude most phytoplankton taxa from biomass samples. Utilization of the No. 10 net would also eliminate most rotifersfrom biomass estimates; however, rotifers often comprise a small portion of the total zooplankton biomass (Kasymov et al. 1972; Pederson et al. 1976).The resulting relationship between microcrustacean density and biomass is O) usually well-defined (Cowell 1967) and may provide more reliable biomass O) estimates for comparison with other reservoirs.

6 5.3

SUMMARY

AND CONCLUSIONS Zooplanklon densities in the ta1lwaters of John Redmond Reservoir ranged from 42,018/nrm in April to 234,312/in in October. The community was dominated by rotifers, primarily the genera Brachionus, Keratella, Polyarthra, and Synchaeta.

These taxa have been important components in the river zooplankton community during past studies. Immature copepods were seasonally important comprising 42.7 percent and 51.0 percent in April and June, respectively.

Principal adult copepods included Cyclops vernalis and Diaptomus siciloides although both generally comprised less than five percent of the mean total zooplankton standing crop. Cladocerans were unimportant -contributors to the total zooplankton assemblage in the tailwaters never comprising more than 1.6 percent of the total zooplankton.

Immature daphnids accounted for the majority of cladocerans identified.

Mean microcrustacean densities in the tailwaters continued to decline during the current study. The reduced densities are probably not related to construction activities at WCGS, but to several biotic and abiotic factors including water exchange rates, food supply, fish predation and the influence of zooplankton recruitment from upstream reservoirs which result in year-to-year variations in zooplankton abundance.

Zooplankton densities generally decreased at downstream Locations 10 and 4 which followed trends established during past studies. A relationship between reservoir water discharge and mean total zooplankton density at the downstream locations was evident although it was not statistically significant (P > 0.05). As river flows were increased, reservoir zooplankton were transported downstream resulting in similar zooplankton densities among locations.

Decreases in zooplankton abundance were most apparent during periods of low reservoir discharge.

Apparent autochthonous zooplankton production occurred between downstream locations when river flows were low since pools conducive to zooplankton production were formed. This resulted in the appearance of several littoral taxa not present in the tailwaters of John Redmond Reservoir.

Mean total annual zooplankton density in the cooling lake was 401,494/m 3.The zooplankton community followed normal seasonal variations in abundance with highest densities observed in early spring and lowest densities occurring in the summer. Rotifers, primarily Keratella and Polarthra and immature copepods combined to account for over 89 percent of the mean yearly zoo-plankton standing crop. Sixty-five taxa including 6 copepod, 12 cladoceran, and 23 rotifer genera were identified.

Zooplankton abundance and taxa diversity were higher at Location 2 than at Location 6 due primarily to the littoral habitat at Location 2 when compared to the limnetic habitat at Location 6. The mean annual zooplankton d y-weight biomass was 1,55.8 mg/m3 with a range from 29.2 mg/in to 337.8 mg/nT at Location I6 in -October and February, respectively.

5-14 EA Engineering, Science, and Technology, Inc.WOLF CREEK GENERATING STATION OPERATIONAL PHASE ENVIRONMENTAL MONITORING PROGRAM, FINAL REPORT Prepared for Wolf Creek Nuclear Operating Corporation Prepared by EA Engineering, Science, and Technology, Inc.Great Plains Regional Office September/1988

/-t L~L VOLF CREEK GENERATING STATION OPERATIONAL PHASE ENVIRONM ENTAL MONITORING PROGRAM, FINAL REPORT Prepared for Volf Creek Nuclear Operating Corporation Prepared by EA Engineering, Science, and Technology, Inc.Great Plains Regional Office Ronald J. Sockelman, Project Manager Barry Sth, Vice President Director of Operations

/o 0 Date IP 10,111/9 Date September/1988 CONTENTS Page 1. INTRODUCTION 1-1 1.1 Objectives and Scope 1-1 1.2 Station Description 1-3 1.3 Status of Wolf Creek Generation Station 1-4 1.4 Description of Study Area 1-5 1.4.1 Neosho River 1-5 1.4.2 Cooling Lake 1-6 1.5 References 1-9 2. WATER QUALITY 2-1 2.1 Introduction 2-1 2.2 Methods 2-1 2.3 Results and Discussion 2-4 2.3.1 Neosho River Surface Water 2-4 2.3.2 Cooling Lake Water Quality 2-8 2.3.3 Groundwater Quality 2-13 2.4 Summary and Conclusions 2-14 2.4.1 Neosho River Water Quality Studies 2-14 2.4.2 WCCL Water Quality Studies 2-15 2.4.3 Groundwater Studies 2-15 2.5 References 2-17 3. PLANKTON PRODUCTIVITY 3-1 3.1 Introduction 3-1 3.2 Methods 3-2 3.3 Results and Discussion 3-4 3.3.1 Neosho River Phytoplankton 3-4 3.3.2 Cooling Lake Phytoplankton 3-6 3.3.3 Cooling Lake Zooplankton 3-9 3.4 Neosho River Phytoplankton Studies 3-12 3.4.1 Neosho River Phytoplankton Studies 3-12 3.4.2 WCCL Plankton Studies 3-12 3.5 References 3-15 CONTENTS (Cont.)Page 4. MACROINVERTEBRATES 4-1 4.1 Introduction 4-1 4.2 Methods 4-2 4.3 Results and Discussions 4-5 4.3.1 Neosho River Macroinvertebrates 4-5 4.3.2 Cooling Lake Macroinvertebrates 4-9 4.3.3 Corbicula Distribution and Abundance 4-14 4.4 Summary and Conclusions 4-16 4.4.1 Neosho River Macroinvertebrate Studies 4-16 4.4.2 WCCL Macroinvertebrate Studies 4-17 4.4.3 Asiatic Clam Corbicula 4-18 4.5 References 4-19 5. FISHERIES 5-1 5.1 Introduction 5-1 5.2 Field and Analytical Procedures 5-2 5.3 Results and Discussion 5-4 5.3.1 Overview 5-4 5.3.2 Electrofishing 5-6 5.3.3 Seining 5-9 5.4 Summary and Conclusions 5-11 5.5 References 5-13 APPENDIX A: SUPPLEMENTAL WATER QUALITY FIGURES APPENDIX B: 1987 DATA TABLES LIST OF TABLES Number Title Page 1-1 Summary of average flows (cfs) in the Neosho River at 1-1i Burlington, Kansas, 1961-1987 1-2 Weekly Wolf Creek Cooling Lake elevations, 1982-1987 1-12 2-1 Summary of locations sampled at Wolf Creek Generating 2-37 Station, 1974-1987 2-2 Wells sampled as part of groundwater program near Wolf 2-38 Creek Generating Station, 1974-1987 2-3 Summary of parameters analyzed in groundwater samples 2-39 from wells near Wolf Creek Generating Station, 1974-1987 2-4 Number of analyses conducted on surface water quality 2-40 samples collected near Wolf Creek Generating Station, 1974-1987 2-5 Annual mean concentrations of selected water quality 2-41 parameters for the Neosho River and Wolf Creek Cooling Lake (WCCL) near Wolf Creek Generating Station, 1974-1987 2-6 Mean concentrations of water quality parameters for wells 2-42 sampled near Wolf Creek Generating Station, 1977-1987 3-1 Phytoplankton standing crop and productivity at Locations 3-25 1, 10, and 4 in the Neosho River near Wolf Creek Generating Station, Burlington, Kansas, 1973-1979 3-2 Phytoplankton standing crop and productivity at Locations 3-26 1, 10, and 4 in the Neosho River near Wolf Creek Generating Station Burlington, Kansas, 1980-1987 3-3 Phytoplankton standing crop and productivity at Locations 3-27 2, 6, and 8 in the cooling lake of Wolf Creek Generating Station Burlington, Kansas, 1981-1987 3-4 Plankton standing crops for selected thermally influenced 3-28 lakes in midwestern and great plains states 3-5 Zooplankton biomass standing crop at Locations 2, 6, and 8 3-29 in the cooling lake of Wolf Creek Generating Station, Burlington, Kansas 1981-1987 4-1 Occurrence of macroinvertebrates at sampling locations in 4-24 the Neosho River and cooling lake near Wolf Creek Generating Station, Burlington, Kansas LIST OF TABLES (Cont.)Number Title Page 4-2 Annual number of macroinvertebrate taxa collected from 4-30 Locations 1, 10, and 4 in the Neosho River and Locations 2, 6, and 8 in the cooling lake of Wolf Creek Generating Station, Burlington, Kansas 1973-1987 4-3 Macroinvertebrate density and diversity in ponar collec- 4-31 tions from Locations 10 and 4 in the Neosho River near Wolf Creek Generating Station, Burlington, Kansas, 1973-1987 4-4 Select macroinvertebrate taxa densities in ponar collec- 4-33 tions from Locations 10 and 4 in the Neosho River near Wolf Creek Generating Station, Burlington, Kansas, 1973-1987 4-5 Select macroinvertebrate taxa densities in ponar collec- 4-35 tions from Locations 2, 6, and 8 in the cooling lake of Wolf Creek Generating Station, Burlington, Kansas, 1981-1987 4-6 Macroinvertebrate density and diversity in ponar collec- 4-36 tions from Locations 2, 6, and 8 in the cooling lake of Wolf Creek Generating Station, Burlington, Kansas, 1981-1987 4-7 Summary of asiatic clam (Corbicula fluminea) abundance in 4-37 ponar grabs from two locations on the Neosho River, Burlington Kansas 4-8 Summary of samples collected during survey for asiatic 4-38 clams in the vicinity of Wolf Creek Generating Station, 30 September

-1 October 1987 5-1 Summary of sampling schedule for surveys of the Neosho 5-14 River near Wolf Creek Generating Station 5-2 Checklist of fishes collected from the Neosho River near 5-15 Wolf Creek Generating Station, Burlington, Kansas 5-3 Relative abundance of thirteen predominant fishes in com- 5-17 bined electrofishing and seining catches from the Neosho River near Wolf Creek Generating Station, Burlington, Kansas 5-4 Number and relative abundance of fish collected by electro- 5-18 fishing in the Neosho River near Wolf Creek Generating Station, Burlington, Kansas, 1977-1982 and 1985-1987 5-5 Summary of preoperational (1977-82) and operational (1985- 5-19 87) catch data for predominant fishes collected by electro-fishing in the Neosho River near Wolf Creek Generating Station, Burlington, Kansas 5-6 Average electrofishing CPE for predominant fishes in the 5-20 Neosho River near Wolf Creek Generating Station, Burlington, Kansas, 1977-1982 and 1985-1987 LIST OF TABLES (Cont.)Number Title Page 5-7 Number of selected fishes collected by seining in the 5-21 Neosho River near the Wolf Creek Generating Station, Burlington, Kansas, 1973 through 1981 and 1985 through 1987 5-8 Summary of preoperational and operational catch data for 5-22 predominant fishes collected by seining in the Neosho River near Wolf Creek Generating Station, Burlington, Kansas 5-9 Number of predominant fishes collected by seining at three 5-23 locations on the Neosho River near Wolf Creek Generating Station, 1976-82, and 1985-87 LIST OF FIGURES Number Title Page 1-1 Aquatic sampling locations in the vicinity of Wolf Creek 1-10 Generating Station 2-1 Total suspended solids in the Neosho River and WCGS 2-18 Cooling Lake near Wolf Creek Generating Station 2-2 Calcium concentrations in the Neosho River and WCGS 2-19 Cooling Lake near Wolf Creek Generating Station 2-3 Magnesium concentrations in the Neosho River and WCGS 2-20 Cooling Lake near Wolf Creek Generating Station 2-4 Soluble orthosphosphate concentrations in the Neosho 2-21 River and WCGS Cooling Lake near Wolf Creek Generating Station 2-5 Total iron concentrations in the Neosho River and WCGS 2-22 Cooling Lake near Wolf Creek Generating Station 2-6 Total alkalinity concentrations in the Neosho River and 2-23 WCGS Cooling Lake near Wolf Creek Generating Station 2-7 Nitrate concentrations in the Neosho River and WCGS 2-24 Cooling Lake near Wolf Creek Generating Station 2-8 Total dissolved solids in the Neosho River and WCGS 2-25 Cooling Lake near Wolf Creek Generating Station 2-9 Sulfate concentrations in the Neosho River and WCGS 2-26 Cooling Lake near Wolf Creek Generating Station 2-10 Total organic nitrogen concentrations in the Neosho 2-27 River and WCGS Cooling Lake near Wolf Creek Generating Station 2-11 Nickel concentrations in the Neosho River and WCGS 2-28 Cooling Lake near Wolf Creek Generating Station 2-12 Ammonia concentrations in the Neosho River and WCGS 2-29 Cooling Lake near Wolf Creek Generating Station 2-13 Chemical oxygen demand and in the Neosho River and WCGS 2-30 Cooling Lake near Wolf Creek Generating Station 2-14 Biochemical oxygen demand in the Neosho River and WCGS 2-31 Cooling Lake near Wolf Creek Generating Station LIST OF FIGURES (Cont.)Number Title Page 2-15 Copper concentrations in the Neosho River and WCGS 2-32 Cooling Lake near Wolf Creek Generating Station 2-16 Chromium concentrations in the Neosho River and WCGS 2-33 Cooling Lake near Wolf Creek Generating Station 2-17 Surface water temperatures in WCGS Cooling Lake near Wolf 2-34 Creek Generating Station 2-18 Depth profiles of water temperature at Location 6 in WCGS 2-35 Cooling Lake near Wolf Creek Generating Station, 1985-1987 2-19 Depth profiles of dissolved oxygen at Location 6 in WCGS 2-36 Cooling Lake near Wolf Creek Generating Station, 1985-1987 3-1 Phytoplankton standing crop trends in the Neosho River 3-18 near Wolf Creek Generating Station, 1973-1987 3-2 Phytoplankton productivity trends in the Neosho River 3-19 near Wolf Creek Generating Station, 1973-1987 3-3 Phytoplankton standing crop trends in the cooling lake 3-20 near Wolf Creek Generating Station, 1981-1987 3-4 Phytoplankton productivity trends in the cooling lake near 3-21 Wolf Creek Generating Station, 1981-1987 3-5 Zooplankton dry weight standing crop trends in the cooling 3-22 lake near Wolf Creek Generating Station, 1981-1987 3-6 Zooplankton ash free dry weight trends in the cooling lake 3-23 near Wolf Creek Generating Station, 1981-1987 3-7 Relationship between phytoplankton and zooplankton standing 3-24 crop in the cooling lake near Wolf Creek Generating Station 4-1 Macroinvertebrate density and diversity in the Neosho River 4-20 near Wolf Creek Generating Station, 1973-1987 4-2 Spatial and temporal trends for major benthic groups in 4-21 the Neosho River near Wolf Creek Generating Station, 1973-1987 4-3 Spatial and temporal trends in total taxa and major benthic 4-22 groups in the Cooling Lake at Wolf Creek Generating Station, 1981-1987 4-4 Macroinvertebrate density and diversity in the cooling lake 4-23 at Wolf Creek Generating Station, 1981-1987

1. INTRODUCTION 1.1 OBJECTIVES AND SCOPE Annual environmental monitoring studies associated with Wolf Creek Generating Station (WCGS) have been conducted since 1973 when baseline information necessary for an Environmental Report was collected.

Subsequent studies of the aquatic and terrestrial biota near WCGS fulfilled commitments made to the Nuclear Regulatory Commission (NRC) prior to issuance of a construction permit. Studies from 1974 through 1980 provided additional baseline information and monitored areas that could be impacted by construction activities (Kansas Gas and Electric 1981; Hazleton Environmental Sciences 1980; Ecological Analysts, Inc. 1981). Completion of the main dam for the WCGS cooling lake (WCCL) initiated a four-year preoperational study (1981-1984) that provided baseline water quality and biological data from the WCCL (Ecological Analysts, Inc. 1982, 1983, 1984; EA 1985). Operational studies began in 1985 and represented continuation of the 1984 WCCL study and reinstatement of the monitoring program on the Neosho River which was curtailed after 1981. This report summarizes preoperational (i.e. 1973-1984) and operational (1985 -1987) data associated with the VCGS environmental monitoring program. Two previous annual reports (EA 1986, 1987) summarized operational data collected from August 1985 through 1986. This summary report includes additional operational data collected from February through August 1987 that met a commitment by Wolf Creek Nuclear Operating Corporation to conduct an environmental monitoring program for two years after commercial 1-1 operation of WCGS commenced.

i The major objective of the Operational Environmental Monitoring Program for WCGS was to document potential environmental changes in the WCCL and Neosho River which could result from operation of WCGS. Specific objectives of the study were to: 1. document concentrations of general water quality parameters, aquatic nutrients, organically-derived materials and certain trace metals in the Neosho River and WCCL 2. monitor bottom to surface dissolved oxygen profiles on WCCL 3. determine general groundwater quality in the vicinity of WCGS 4. characterize the benthic community within the Neosho River and WCCL 5. determine phytoplankton productivity of the Neosho River and WCCL 6. determine zooplankton biomass in the WCCL 7. assess fish populations in the Neosho River In addition to the above specific objectives, the studies documented naturally occurring variations in the aquatic community of the Neosho River and WCCL.1-2 1.2 STATION DESCRIPTION Wolf Creek Generating Station is located in Coffey County approximately 5.6 kilometers northeast of Burlington, Kansas. Upon completion in 1985, the station employed a pressurized water reactor to produce 1,150 megawatts (net output) of electrical power. The site encompasses 9,818 acres of range, cropland, and woodland typical of southeastern Kansas. Within this, the plant site occupies 135 acres and the WCCL approximately 5,090 acres. A once-through cooling system, utilizing water from the WCCL is used by the WCGS. The cooling lake was formed by impounding Wolf Creek approximately 8.8 kilometers upstream from its confluence with the Neosho River. A surface elevation of 1,087 feet above sea level is maintained in the cooling lake by precipitation and runoff in the Wolf Creek watershed and makeup water from the Neosho River. A makeup water pump house (MUSH) on the Neosho River in the tailwaters of John Redmond Reservoir provides water to the cooling lake via an underground pipeline (Figure 1-1). The auxiliary raw water pumps have withdrawn 1 to 2 cfs from the JRR tailwaters since WCGS became operational except from 17-24 September and 15-22 October 1985; 24-26 January, 26-27 July, and 15-20 October 1986; and 14-15 January 1987. Except for testing, the make up water pumps ran only on 4-11 August 1987, when pumping rates averaged 100 cfs.1-3 1.3 STATUS OF WOLF CREEK GENERATION STATION Wolf Creek Generating Station was considered 99 percent complete by the end of 1984. The circulating water system operated extensively during start-up testing and extended into the 1984-1985 winter. "Hot Functional" testing was completed during the start-up activities.

Construction activities that could affect the Neosho River were completed in 1980 and work on the plant site was limited primarily to site cleanup, much of which was completed in 1984.The following milestones highlight activities during 1985, the year WCGS began operation:

WCGS received its low power license on 11 March 1985 and immediately began fuel loading.Initial criticality was reached 22 May 1985 and the full power license was granted 4 June 1985.Power was first generated on 12 June 1985 with 100 percent power achieved on 8 August 1985.Commercial operation of WCGS was declared on 3 September 1985.Start up/testing and operational activities during 1985 involved use of WCCL for cooling water and dissipation of waste heat. Heat rejection rates were 1-4 greatest from August 1985, when 100 percent power was achieved, through December.

WCGS operated throughout 1986 and most of 1987 except for maintenance and refueling outages. Outages in 1986 occurred in April (16.6 days) and from 16 October through 21 December (66.3 days) when refueling activities were completed.

In 1987 outages occurred in January (4 days), April (1 day), June (1 day), July (5 days), early September (1 day), and from 28 September through 31 December (95 days).

1.4 DESCRIPTION

OF STUDY AREA 1.4.1 Neosho River The Neosho River is a relatively slow meandering stream that rarely exceeds a gradient of 1 m/km (Prophet 1966). The river was significantly altered in 1964 with the completion of John Redmond Dam. River flow in the study area is dependent upon discharge from John Redmond Reservoir (JRR) which is regulated by the U.S. Army Corps of Engineers.

Substrates in the tailwaters of the JRR are layered limestone, shale, and sandstone bedrock. Flow is variable (Table 1-1) and entirely dependent upon reservoir releases.

Pools, gravel bars, and riffles characterize the lower river near the confluence with Wolf Creek.Substrates in the riffle habitats are rock, rubble, and gravel, whereas the pools are characterized by bedrock overlaid by silt.Four locations in the Neosho River were sampled (Figure 1-1). Location 1 was in the tailwaters of John Redmond Dam near the MUSH. The bottom substrate was 1-5 U bedrock, with rock riprap along the banks. Pools and riffles characterized Location 10 which was 0.7 kilometers upstream of the confluence with Wolf Creek. The riffles had substrates of rock, rubble, and gravel, whereas the pools were characterized by bedrock overlaid with silt. A riffle located approximately 0.5 kilometer below the confluence with Wolf Creek constituted Location 11. The riffle consisted of small rubble, gravel, sand, and silt.Location 4, 1.3 kilometers downstream of the confluence with Wolf Creek, was comprised of a deep pool and a shallow gravel bar. The substrate of the pool was silt and sand, whereas the gravel bar consisted of sand and gravel.1.4.2 Cooling Lake The WCCL was formed by one main earth-rolled dam across Wolf Creek and five perimeter saddle dams (Figure 1-1). The top of the main dam is at elevation 1,100 feet above mean sea level (msl) and each dam has a 3 to 1 slope on both the upstream and downstream faces. The upstream slope of each dam is riprapped for protection against wind-generated wave erosion while the downstream slope is seeded. Descriptions of the service and auxiliary spillways, low-level outlet works, and operation of the cooling lake are described in the operating license stage environmental report for WCGS (Kansas Gas and Electric 1981).Filling of the WCCL began in October 1980 and continued through November 1981.Approximately 23 billion gallons of water were pumped through the makeup water screenhouse in 1981 with monthly pumping rates varying from nearly 49 million 1-6 gallons in April to 3.4 billion gallons in October. Storage water was purchased from John Redmond Reservoir at the rate of 26.5 MGD (41 cfs) through a contract with the Kansas Water Resources Board. The cooling lake elevation rose from 1,050 to 1,079.5 msl during 1981 resulting in a surface area increase from 890 to 3,900 acres. Pumping continued in January, February, and March 1982 aided by surface water runoff which filled the cooling lake to normal operating level (1,087 msl) by June 1982. Storage elevations from 1983 through 1987 have been stable varying from 0.8 feet below to 2.0 feet above the normal water elevation of 1,087.0 msl (Table 1-2). Storage elevations were generally lowest during the winter (December-January) with peak elevations in May and June following spring runoff. Storage elevations during 1987 were generally above normal through summer and then declined to the annual minimum in late November.

Makeup water from John Redmond Reservoir has only been required once (October 1987) since initial lake filling, but as discussed in Section 1.2, the auxiliary water pump(s) were used all but 16, 10, and 2 days in 1985, 1986, and 1987 respectively.

Stabilization of lake storage elevations has favored the development of submergent and emergent aquatic macrophytes.

The small drainage area associated with the WCCL limits runoff into the WCCL which results in good water clarity, expanding the zone of submerged macrophyte colonization to depths up to 12 feet. Areas afforded wind protection by the baffle dikes and saddle dams, and along other protected shorelines have stands of Potamogeton and to a limited extent, Chara. Stands of cattails, Typha spp., also are continuing to develop. Establishment of aquatic macrophytes should benefit 1-7 the cooling lake fishery by providing cover and shelter and could encourage i i1 production in the littoral zooplankton and macroinvertebrate communities.

Aquatic sampling locations were established upstream (Location

2) and down-stream near the main dam (Location 6). Location 2, the location expected to be most affected by station discharges, was moved in 1982 from the old Wolf Creek channel near the makeup water discharge structure to an area adjacent to the creek channel, approximately 0.5 miles upstream of the 1981 location.This change was made to accommodate continued filling of the lake.Differences between these locations were typical of upstream and downstream reaches of reservoirs regarding depth and turbidity.

Location 8, located east of Baffle Dike A (Figure 1-1) adjacent to the Ultimate Heat Sink, was added as an intake location for the 1984 study.1-8

1.5 REFERENCES

Ecological Analysts, Inc. 1981. Wolf Creek Generating Station Construction Environmental Monitoring Program, April 1980 -January 1981. Report to Kansas Gas and Electric Company, Wichita.Ecological Analysts, Inc. 1982. Wolf Creek Generating Station Construction Environmental Monitoring Program, February 1981 -January 1982. Report to Kansas Gas and Electric Company, Wichita.Ecological Analysts, Inc. 1983. Wolf Creek Generating Station Environmental Monitoring Program, February 1982-December 1982. Report to Kansas Gas and Electric Company, Wichita.Ecological Analysts, Inc. 1984. Wolf Creek Generating Station Preoperational Phase Environmental Monitoring Program, March 1983 -December 1983. Report to Kansas Gas and Electric Company, Wichita.EA Engineering, Science, and Technology, Inc. 1985. Wolf Creek Generating Station Operational Phase Environmental Monitoring Program, February 1984 -December 1984. Report to Kansas Gas and Electric Company, Wichita.EA Engineering, Science, and Technology, Inc. 1986. Wolf Creek Generating Station Operational Phase Environmental Monitoring Program, March 1985 -December 1986. Report to Kansas Gas and Electric Company, Wichita.EA Engineering, Science and Technology, Inc. 1987. Wolf Creek Generating Station Operational Phase Environmental Monitoring Program, January 1986 -December 1986. Report to Wolf Creek Nuclear Operating Corporation, Burlington, Kansas.Hazleton Environmental Sciences.

1980. Final Report of Construction Environmental Monitoring Program, March 1979 -February 1980. Report to Kansas Gas and Electric Company, Wichita.Kansas Gas and Electric.

1981. Wolf Creek Generating Station Environmental Report (operating license stage). Wichita, Kansas. 2 vols.Prophet, C.W. 1966. Limnology of John Redmond Reservoir, Kansas. Emporia Stat. Res. Stud. 15(2):5-27.

1-9

!0 MILES I 1 2 M Groundwater Sampling Location 0 B-12 3 4 N Ultimate Heat Sink.- Baffle Dike A LAKE Figure 1-1. Aquatic sampling locations in the vicinity of Wolf Creek Generating Station.1-10 TABLE 1-1

SUMMARY

OF AVERAGE FLOWS (CFS) IN THE NEOSHO RIVER AT BURLINGTON, KANSAS, 1961-1987 Year JAN FEB MAR APR MAY JUN JUL AUG SEP OCT NOV DEC Mean 1961 ------2243 475 3627 1065 245 538 -1962 2306 3521 2021 766 1414 4746 998 407 6599 1528 597 461 2090 1963 588 376 1154 378 318 664 686 69 31 50 87 82 374 1964 79 79 75 804 778 1639 135 99 32 32 1173 531 452 1965 318 360 1676 1321 324 9988 4211 151 4163 1065 245 538 2023 1966 493 547 418 1114 480 771 66 192 43 31 33 25 348 1967 21 23 25 514 65 2827 6944 557 1499 4583 966 635 1569 1968 544 340 211 1715 1162 2086 817 1917 115 1704 1741 997 1113 1969 1024 1231 2200 2603 6864 4066 7332 847 1189 1845 1085 1259 2645 1970 514 335 248 4402 2077 5156 424 75 724 1558 301 823 1381 1971 968 990 1771 235 1957 8449 4817 1263 42 78 1709 1428 1977 1972 487 332 103 454 4215 181 1604 188 154 45 235 941 753 1973 3578 5363 7637 7772 6203 3397 598 232 1342 11544 7543 3802 4913 1974 3039 1245 2368 2763 2759 3089 209 152 1330 413 2762 1390 1790 1975 1102 2444 2242 2723 771 6805 2342 186 345 124 93 653 1637 1976 228 60 50 359 3260 1363 1508 45 46 49 45 43 592 1977 45 46 47 48 1491 4862 6626 1114 1999 605 1923 484 1614 1978 300 812 2960 3484 1084 852 952 58 39 24 19 17 882 1979 18 437 3090 1493 558 2875 4085 587 129 68 983 575 1247 1980 355 904 1360 6308 499 171 150 53 58 36 18 26 819 1981 19 24 14 21 271 1786 3378 866 1032 537 4393 1801 1181 1982 513 4323 1330 610 4879 7385 1750 204 70 39 32 124 1748 1983 383 1092 736 6750 7741 5325 1344 70 39 31 268 735 2039 1984 436 847 3972 8191 4093 2813 1050 60 32 24 150 616 1852 1985 1105 695 5119 1191 3175 7187 1919 2537 3371 8510 6344 2807 3711 1986 785 2023 549 2083 2745 598 3343 1123 740 7931 2071 1631 2144 1987 860 2152 7581 3725 979 2294 1826 891 760 183 479 1965 1971 Note: Data from USGS gaging station except AUG-DEC 1987 data from WCGS water reports (JRR outflow).1-11 TABLE 1-2 WEEKLY WOLF CREEK COOLING LAKE ELEVATIONS, 1982-1987 Month Week JAN 1 2 3 4 FEB 1 2 3 4 MAR I 2 3 4 5 APR 1 2 3 4 5 MAY 1 2 3 4 1982 1,079.5 1,079.5 1,079.5 1,079.5 1,080.3 1,080.3 1,080.4 1,079.5 1,080.4 1,080.6 1,080.8 1,080.9 1,081.0 1,081.3 1,082.0 1,082.4 1,082.7 1,083.6 1,084.8 1,086.5 1,087.5 1,087.6 1,087.6 1,087.4 1983 1,087.0 1,087.0 1,087.0 1,087.0 1,087.0 1,087.3 1,087.5 1,087.8 1,087.8 1,087.1 1,087.5 1,087.8 1,087.8 1,088.5 1,088.4 1,088.1 1,088.3 1,088.6 1,088.2 1,088.0 1,088.4 1,088.5 1,088.5 1,088.5 1,088.5 1984 1985 (a) 1986 (a) 1987(1,087.0 1,087.0 1,087.0 1,087.0 1,087.0 1,087.0 1,087.0 1,087.0 1,087.0 1,087.0 1,087.6 1,087.0 1,088.0 1,088.0 1,088.4 1,088.0 1,088.0 1,088.0 1,088.0 1,088.0 1,088.0 1,088.6 1,088.0 1,088.0 1,086.6 1,086.9 1,087.0 1,087.8 1,087.0 1,087.0 1,087.0 1,087.3 1,088.0 1,088.0 1,088.0 1,088.0 1,088.1 1,088.2 1,088.2 1,088.0 1,088.0 1,088.0 1,088.0 1,088.0 1,088.1 1,088.2 1,088.6 1,088.7 1,088.5 1,088.2 1,088.2 1,088.0 1,087.8 1,087.8 1,088.0 1,088.0 1,087.9 1,087.5 1,087.5 1,087.6 1,087.5 1,087.5 1,087.4 1,087.4 1,087.5 1,087.5 1,087.5 1,087.5 1,087.5 1,087.6 1,087.6 1,087.7 1,087.5 1,087.2 1,087.0 1,087.0 1,087.6 1,087.5 1,087.6 1,087.5 1,087.5 1,087.5 1,087.5 1,087.5 1,088.0 1,088.0 1 088.0 1,088.5 1,088.0 1,087.8 1,088.0 1,088.0 1,088.0 1,088.0 1,087.5 1,087.1 1,088.0 1,089.0 1,088.0 1,088.0 1,088.0 1,087.5 a) Month Week JUL 1 2 3 4 5 AUG 1 2 3 4 5 SEP 1 2 3 4 5 OCT 1 2 3 4 NOV 1 2 3 4 5 DEC 1 2 3 4 5 1982 1983 1984 1 9 8 5 (a) 1 9 8 6 (a) 1 9 8 7 (a)1,087.5 1,087.5 1,088.0 1,087.9 1,087.8 1,087.8 1,087.5 1,087.5 1,087.5 1,087.5 1,087.5 1,087.5 1,087.2 1,087.2 1,087.0 1,087.3 1,087.0 1,087.0 1,087.1 1,087.1 1,087.0 1,086.8 1,087.0 1,086.8 1,086.7 1,087.0 1,087.0 1,088.2 1,088.1 1,087.4 1,087.4 1,087.4 1,087.1 1,087.3 1,087.1 1,087.0 1,087.0 1,086.6 1,087.0 1,087.0 1,086.5 1,086.6 1,086.6 1,086.7 1,086.8 1,086.6 1,086.8 1,087.0 1,086.1 1,087.0 1,086.5 1,086.7 1,088.0 1,088.0 1,087.7 1,087.7 1,087.5 1,087.4 1,087.3 1,087.1 1,087.0 1,087.0 1,087.0 1,087.0 1,086.5 1,086.5 1,086.5 1,086.5 1,086.5 1,086.5 1,086.5 1,086.5 1,086.5 1,086.5 1,086.3 1,086.4 1,086.4 1,086.5 1,088.1 1,088.0 1,088.0 1,087.9 1,087.6 1,087.6 1,087.5 1,087.2 1,088.0 1,088.0 1,088.0 1,087.8 1,087.7 1,087.5 1,087.6 1,088.4 1,088.2 1,088.0 1,088.1 1,088.0 1,087.9 1,088.0 1,088.0 1,088.0 1,088.0 1,088.0 1,087.0 1,087.4 1,087.5 1,087.2 1,087.0 1,087.0 1,087.0 1,087.0 1,086.9 1,086.6 1,086.5 1,086.5 1,086.5 1,087.1 1,087.9 1,087.4 1,087.6 1,086.9 1,087.5 1,087.5 1,087.5 1,087.3 1,087.4 1,087.4 1,087.6 1,087.6 1,087.5 1,087.5 1,087.5 1,087.0 1,087.1 1,087.4 1,087.3 1,087.5 1,087.4 1,087.2 1,087.0 1,087.0 1,087.5 1,086.5 1,086.5 1,086.4 1,086.5 1,086.9 1,086.5 1,086.4 1,086.2 1,086.5 1,086.6 1,086.6 1,087.2 1,087.2 I--'JUN 1 2 3 4 5 fole: Readings taken once per week.a Weekly mean elevation.

2. WATER QUALITY

2.1 INTRODUCTION

Water quality studies have been conducted on the surface water and groundwater in the area of the WCGS since 1974. These studies were designed to provide a database reflecting existing water quality conditions which could be used as a comparison for conditions observed during construction and ultimately operation of the WCGS. Water quality parameters selected for analysis throughout the various study phases were chosen based on state water quality standards, drinking water standards and parameters predicted to change or be added to the system during operation as described in the operating license stage environmental report (ER-OLS) for this project.2.2 METHODS The selection of sampling locations for the study was modified over time as the scope of the study dictated (Table 2-1). Early years included sample collection in John Redmond Reservoir, the Neosho River and Wolf Creek. After January 1976 the John Redmond Reservoir location was dropped and samples were collected immediately below the dam in the tailwaters and at selected points downstream in the Neosho River. Samples were collected from the Wolf Creek watershed until 1981. Following closure of the cooling lake dam, Wolf Creek locations were changed to selected locations in the cooling lake. Two locations were sampled from 1981 through 1983 and three locations have been sampled from 1984 through 1987.2-1 Groundwater samples for this study program have been collected from as many as 8 wells in a given study year. A total of twelve different wells have been used in the program. All wells sampled were existing, shallow aquifer wells that varied from hand dug and brick lined construction to drilled wells with steel or plastic casing. A list of the wells sampled each year is provided in Table 2-2. Starting in 1980 four wells were selected from the original group as the final set of groundwater monitoring wells for the project. These four wells B-12, C-20, C-49 and D-65 have been sampled each year since 1979. The parameters analyzed from groundwater samples were fairly consistent over the study period (Table 2-3).Over the fourteen year period of this study as many as 41 individual analytical parameters have been determined for surface waters at one time or another.Table 2-4 provides a summary of the parameters and the number of samples of surface water analyzed each year of the study. In excess of 18,000 individual analyses were conducted on water samples, excluding quality control replicates and multiple dissolved oxygen and temperature data from surface to bottom profiles conducted in the cooling lake. Analytical methods used by laboratories conducting chemical analysis of water samples for these studies were generally the same throughout the study period. Methods used prior to 1979 were based primarily on Standard Methods for Water and Wastewater (APHA et al. 1971 and 1975). Beginning in 1979 methods also followed protocols established by the U.S. EPA (1979) in Methods for Chemical Analysis of Water and Wastes.Starting with the 1980 study, bacteriological analyses were conducted using the delayed incubation method (APHA 1980). Water samples were filtered in an 2-2 onsite laboratory, placed on holding media, and returned to EA's lab for incubation.

During this same period the oil and grease solvent used for extraction was changed from hexane to freon to comply with U.S. EPA 1979 methods.Selection of parameters for presentation in this summary report was first limited to those included in the ongoing studies at the WCGS. Next, parameters that were generally at or below analytical detection limits (e.g. oil and grease) were excluded.

There were also a few parameters that exhibited a relatively narrow range of values (e.g. pH) or whose variability mimicked that of other parameters (e.g. specific conductance).

Table 2-5 presents annual values for those parameters not selected for more detailed analysis.

Graphical presentation of the remaining parameters consists of histograms and line drawings depicting annual mean concentrations of the river and lake and annual means by location.

These data were evaluated for long term trends as well as comparisons of pre- and post-construction and preoperational and operation effects. In addition, data comparisons to state and federal water quality standards or criteria were conducted.

Mean concentrations on each date for the combined location data from a given water body (Neosho River or Wolf Creek Cooling Lake) were plotted and are provided in Appendix A. These graphical representations provided a good understanding of the variability in analytic concentration over time and the expected seasonal changes in concentrations of parameters.

2-3 2.3 RESULTS AND DISCUSSION 2.3.1 Neosho River Surface Water Throughout WCGS water quality studies, the general water quality in the Neosho River has been considered good. The major influences on solute concentrations as discussed in the past annual reports has been rainfall or snowmelt conditions and the volume of water released from the John Redmond Dam.Suspended matter in the water column as determined by total suspended solids often showed a direct relationship with levels of related solutes such as trace metals and nutrients.

Annual mean concentrations of general water quality parameters such as color, manganese, chloride, potassium, pH, soluble silica, sodium, turbidity, soluble iron and specific conductance have shown very little variability throughout the study period (Table 2-5).Water temperatures in the Neosho River showed expected seasonal variability (Appendix Table A-i). However, annual mean water temperatures varied little throughout the study and often reflected flow conditions (e.g. warmer temperatures were often observed during summer months with lower flow conditions than during summer months when flows were high).Dissolved oxygen concentrations in the Neosho River varied seasonally as ex-pected. Annual mean concentrations from 1974-1987 ranged from 9.1 to 11.2 mg/l. These concentrations are well above the minimum considered necessary to support aquatic life and meet applicable state and federal water quality cri-teria.2-4 The data from the Neosho River do not reflect any direct construction or operational effects on water quality based on concentrations observed during these studies. A number of parameters exhibited a change in annual mean concentration after 1981. Although some of that change may be due to water system conditions, the changes more probably reflect the change in laboratories conducting the analysis.

Interlaboratory difference is a known variable in all analytical situations and must be considered when evaluating the reported results.Based on annual mean concentrations, total suspended solids (TSS), total alka-linity, calcium (Ca), magnesium (Mg), soluble orthophosphate (O-P04), total iron (Fe) and nitrate showed no consistent long term trend. TSS values were similar all years with the exception of 1981 and 1984 when annual mean concentrations were 2-3 fold higher than normal (Figure 2-1). The 1981 peak was the result of high values in April and October data at all river locations, while the 1984 peak appears to be the result of high values in February, particularly at Location 4. Spatially, there has been no indication of increased TSS values at Location 4 below Wolf Creek since WCGS operation began.Concentrations of Ca, Mg and O-PO 4 were lower in general after 1982 than earlier years (Figures 2-2, 2-3, 2-4). However, the concentrations do not show a distinct trend overall, and the lower values are probably related to the change in laboratories conducting the analysis following the 1981 study period.The actual differences in parameter concentrations before and after 1982 are generally not significant from a chemical standpoint.

Orthophosphate levels generally rose from 1975 to 1981 in the river samples. However, the overall concentration levels of O-PO 4 in the river are low and the mean values presented in the figure represent values reported at or below the detection 2-5 limit. The solute concentrations observed in the river are for the most part a reflection of the quality of water leaving the John Redmond Reservoir.

Total iron levels were slightly higher in recent years in contrast to the solutes previously discussed (Figure 2-5). Total alkalinity (Figure 2-6) was higher than normal in 1987, but nitrate concentrations (Figure 2-7) have remained within the previously observed range of annual values. There have been no spatial differences in these parameters that suggest VCGS operation may be affecting water quality in the Neosho River.Annual mean concentrations of total dissolved solids (TDS), sulfates, total or-ganic nitrogen (TON), and total nickel (Ni) showed overall downward trends during the study period (Figures 2-8 to 2-11). TDS and sulfates concentration curves were most similar (Figures 2-8 and 2-9). Peak sulfate levels observed in 1976 and 1978 appeared to be due primarily to higher values in April and primarily February, respectively.

Location 1 values were slightly higher than downstream locations.

However, little spatial variability of sulfate has been noted overall during the study period (Figure 2-9). Total organic nitrogen (TON) levels peaked in 1984 and 1986 to levels about twice that normally observed in the river. The 1984 peak appeared to be caused by higher levels observed in April and August samples, while in 1986 higher than normal values were observed all year. During 1984, TON values were higher at Location 1 than downstream locations, while the levels observed at Locations 10 and 4 were higher in 1986 (Figure 2-10). For the most part, little or no spatial difference has been observed between Locations 10 and 4 for any of these parameters, indicating the construction and operation of WCGS has not altered water quality in the Neosho River.2-6 Total nickel data is available only for the period 1983-1987.

Concentrations rose from 1983 to 1985, then dropped dramatically in 1986, and 1987 levels were slightly below the 1986 values (Figure 2-11). The high 1985 values may reflect the higher than normal flow conditions observed during much of the 1985 study period. As was observed for most parameters, releases from JRR (Location 1)appeared to control nickel concentrations in this segment of the river, and there were no major spatial differences between Locations 10 and 4, above and below Wolf Creek respectively.

Annual mean concentrations of ammonia have varied throughout the study period in a somewhat cyclic manner (Figure 2-12). Overall there was a slight upward trend in the data; however, the ammonia concentrations observed were low. Peak levels were observed in 1979 and reflect higher values at all locations, particularly in February of that year when discharge from John Redmond Dam was less than 1 cfs. Chemical oxygen demand (COD) and biochemical oxygen demand (BOD) levels followed the same trend of generally increasing values through 1984 then dropped off to near pre-1980 levels in 1985 (Figures 2-13, 2-14).Both parameters showed less variability in annual mean values in the 1974-1979 period. Both parameters peaked in 1984, and this peak appeared to be due primarily to higher values at Location 1 (particularly for COD) during February of that year. Overall the levels of BOD and COD observed in the Neosho River are low and are not indicative of heavy organic loading. The oxygen demand values observed after 1981 may also partially reflect the change in the laboratory conducting the analysis.Copper (Cu) data is available from 1974 through 1987, while chromium (Cr) data is available for the 1983-1987 period only (Figures 2-15, 2-16). Both of 2-7 these parameters were analyzed as total metals and therefore reflect the concentrations in the sample due to dissolved metals in the water as well as any metals in sediment or residue contained in the water column at the time of sampling.

Copper showed little variation in annual mean concentrations from 1974-1981 then began to rise through 1984 (Figure 2-15). Overall the levels of Cu have shown a slightly increasing trend throughout the study. The observed concentration was higher following the 1981 study period and may reflect the change in analytical laboratories.

Chromium concentrations peaked in 1985 at all three river locations.

2.3.2 Cooling Lake Water Quality Water quality sampling in the WCCL began in February 1981 at Locations 2 and 6.Sampling at a third location (Location

8) began in February 1984. Since that time all three locations have been sampled. Water quality of the WCCL during the 1981 sample year reflected primarily the quality of water pumped into the lake from the Neosho River. In addition the concentrations of solutes that were associated with sediment and soils were higher in the lake due to the scouring effects of the pumped water flowing into the lake. As with the water quality in the Neosho River only selected parameters are discussed in the text of this report. Table 2-5 provides a summary of annual mean concentrations of parameters that due to either the consistency of the data, low concentrations, or the fact that they may have been sampled for only a short period of time are not discussed in detail elsewhere in this report.Review of the data in Table 2-5 indicated that for the parameters presented the concentrations of these analyses in the WCCL have changed very little during 2-8 the 1981-1987 period. One exception to that observation is the turbidity levels in the lake. As expected during the 1981 lake filling stage, turbidity levels were 2-3 fold higher than following years due to the disturbance of soils during pumping and also the use of river water to fill the lake.During the 1981 lake filling phase a number of parameters were much higher than subsequent years. Total suspended solids, TDS, sulfates, calcium, total iron, orthophosphate and nitrate levels were all relatively high in 1981 (particularly at Location 2). This 1981 peak reflected concentrations of these parameters in the Neosho River water used to fill the WCCL.Following the 1981 lake filling a number of parameters have shown little change in concentration in the lake over the seven year study period. Total alkalin-ity (Figure 2-6), magnesium (Figure 2-3) and ammonia (Figure 2-12) levels were not substantially higher in 1981 but rather have maintained a generally constant level in the WCCL. Alkalinity increased in 1987 as did ammonia levels; however, the change in actual concentrations was small. Nitrate levels after the 1981 peak dropped to an annual mean level of 0.1 to 0.2 mg/l and have stayed in that range throughout the study (Figure 2-7).Annual mean concentration of total suspended solids (Figure 2-1) and total dis-solved solids (Figure 2-8) declined significantly after the 1981 lake filling phase and have continued to decline slightly over the 1982-1987 study period.Calcium levels have declined more gradually over the 1981-1987 period (Figure 2-2). BOD and COD also have shown a declining trend (except COD in 1987).Levels of these two indicator parameters have been generally low throughout the study period (Figures 2-13 and 2-14). COD concentrations have been less 2-9 consistent than BOD as was the case in the Neosho River data. This may reflect analytical precision more than it does actual water body differences in COD levels. COD values reported from four eastern Nebraska reservoirs ranged from 2.9 to 104 mg/l but usually were in the 15 to 20 mg/l range (Ukpaka 1971). At Sutherland Reservoir in western Nebraska, BOD and COD seasonal values respectively ranged from 2.0 to 3.5 mg/l and from 0.7 to 6.5 for the period 1972 through 1984 (EA 1985).Analysis of water samples for nickel began in 1983 in the WCCL. The concentra-tion in 1983 was the highest observed in the lake to date. Subsequently the observed values have tended to be lower each year (Figure 2-11). An exception to that trend occurred in 1985 when the higher values appeared to be due to samples collected in December of that year. The overall difference in solute concentration observed in nickel and other trace metals was small because the data is presented in ug/l levels or parts per billion. Very minor matrix differences in samples can cause seemingly large differences in concentrations for these parameters.

In addition, these data are for total metals and therefore reflect both dissolved material in the water as well as metals that were adsorbed to residue in the water sample and are therefore not immediately available as a solute in the water matrix.Annual mean concentrations of total iron and sulfates (Figures 2-5 and 2-9)peaked in 1981, dropped significantly in 1982 and have since shown very slight increases in concentration over time. The observed differences in concentration were very low as with most solutes studied in the lake. Soluble orthophosphate levels also dropped from a 1981 peak. However, the concentrations continued to drop slightly through 1984 and have since shown a 2-10 slightly increasing trend (Figure 2-5). The high values observed in 1986 were the result of higher values in August, primarily at Location 2. Orthophosphate levels also reflected higher than actual concentrations, because concentrations reported as less than the analytical detection limit were plotted assuming that value.With two exceptions annual mean levels of total organic nitrogen (TON) have been very stable (Figure 2-10). TON levels in the WCCL increased in 1982 and 1986 by 2-3 fold the usual level. Both of these peaks were observed during the March/April period and probably reflect resuspension of nitrogen-containing materials during spring turnover.Two trace metals exhibiting increased annual mean concentrations in WCCL were copper and chromium.

Annual mean copper values have generally increased steadily since lake filling (Figure 2-15). Copper levels increased considerably in 1985 and, though lower in 1987, were still higher than previously observed.

The higher values for 1986 were observed throughout the year and were observed at all locations, although Location 2 levels were higher than those at 6 and 8 in both years (Figure 2-15). Total chromium levels increased by 10 fold in 1985 within WCCL. The annual mean levels observed in the lake were high at all three locations, and the highest values were recorded in December of 1985 when replicate values were in excess of 100 Pg/l.Unusually high chromium values were also observed in the Neosho River for the same period (Figure 2-16) and the data were considered suspect. If the December 1985 data were eliminated from the calculation of annual means, the peak in 1985 would be in the range of 3.8 vg/l rather than 22 ug/l as plotted.As with orthophosphate values, plotted data for copper and chromium reflect 2-11 data points that were recorded at less than the analytical detection limits, but for calculation purposes were assumed to be observed at the detection limit concentration.

Average lake surface water temperatures were higher during preoperational and operational periods than during lake filling (Figure 2-17). The only exception to that trend was in April sampling at Locations 2 and 6 during 1981, when higher temperatures probably resulted from shallow water depth and the influence of pumped water from the Neosho River. On the average, lake surface has been 1.0 to 5.5 degrees C warmer during plant operation than in the preoperational period. Spatially, temperatures at Location 2 have increased the greatest (3.5 to 7.8 C); Location 6 temperatures have increased by 0.5 to 5.8 C, and Location 8 by 1.5 to 3.5 C. The greatest temperature increases were observed in April. Winter operational temperatures were similar to preoperational temperatures.

A scheduled WCGS outage generally occurs during this period and heat loading to the lake is thus reduced.Depth profiles in WCCL indicated that water temperatures generally decreased with depth. Location 6, being the deepest location, best illustrated temperature changes with depth (Figure 2-18). Temperature differentials in most months were very small, but summer temperatures at Location 6 varied by as much as 9.5 C between surface and bottom (June 1987).Dissolved oxygen profile data collected from 1985 through 1987 indicated that D.O. also generally declined with depth (Figure 2-19). Review of the profiles at Location 6 indicated that the greatest decline in D.O. occurred during summer months. Dissolved oxygen was generally well above the levels necessary 2-12 for the protection of aquatic species in the upper level of the WCCL. However, at depths below 10 meters, D.O. was at or near zero mg/1 in July of 1985 and 1986 and in June 1986 and 1987. In all years concentrations rose to above 5 mg/1 by the August sampling period. The decline in dissolved oxygen during the summer period reflected the effects of warm summer water temperatures and static deep water conditions, a combination of factors that often is conducive to D.O. depletion.

2.3.3 Groundwater Quality Mean concentrations of water quality parameters in groundwater samples collected in 1987 were within the range of concentrations observed in previous years with few exceptions.

Concentrations of hardness, chloride and magnesium were lower in well D-65 than in any previous year as were sulfate concentrat-ions in B-12 and C-49 (Table 2-6). Total iron in well C-49 and specific conductance in wells C-20 and D-65 were also lower than previously observed.Nitrates and soluble iron concentrations were higher in wells D-12 and C-49, respectively, than in previous years. As in previous years, well D-65 generally had the highest levels of dissolved constituents and well C-49 the lowest. The water quality in wells B-12 and C-20 was generally similar.Groundwater quality with the possible exception of well C-49, reflected the age and condition of the wells and the influence of surface water inflows during wet periods.National Primary Drinking Water Criteria for nitrates (10 mg/l) has been exceeded in all four wells at various times during the 11 year study period.The National Secondary Drinking Water Standard for TDS (500 mg/l) was exceeded') 1.'

during all sample periods in all wells. The chloride criteria (250 mg/i) was exceeded in well D-65 in all years except 1987 and in one or more years in all other wells. The total iron criteria (0.3 mg/l) was exceeded most years in all wells except C-49. Well water collected for this study has consistently been very hard with high levels of dissolved constituents.

These observations have not changed since WCCL was filled or since WCGS began operation.

Thus to date there appear to be no effects on groundwater quality due to WCGS in the areas covered by these four groundwater monitoring wells.2.4

SUMMARY

AND CONCLUSIONS 2.4.1 Neosho River Water Quality Studies Water quality studies in the Neosho River near the WCCL have been conducted since 1973. Seasonal mean concentrations of water quality parameters during 1987 were within previously established ranges for the study area. Water quality among river locations was similar though slight natural differences between the John Redmond Reservoir tailwaters (Location

1) and the lower river (Locations 4 and 10) were apparent.

Seasonal differences observed during 1987 and previous years reflect changes in discharge rates from John Redmond Dam and runoff due to local precipitation and snowmelt events. Since filling of the WCCL began in 1981, flows from Wolf Creek into the Neosho River have been limited to seepage, releases for testing of blowdown procedures, and runoff events. There have been no apparent deleterious effects to water quality in the Neosho River due to operation of WCGS based on available water quality monitoring data.2-14 2.4.2 WCCL Water Quality Studies Water quality studies of the WCCL began when the lake was initially filled during 1981. Water quality was greatly influenced by makeup water being pumped from the Neosho River during that year. Since 1981 makeup water has generally been added during routine use of the auxiliary raw water pumps and quarterly testing of the makeup water pumps. Therefore, the WCCL water quality has been generally independent from influence of the Neosho River. Concentrations of water quality parameters are very similar among locations in the cooling lake, with the shallow upstream site (Location

2) slightly different in water quality than near the main dam (Location

6) and the station intake (Location 8).Concentrations of dissolved and suspended constituents continued to show declining trends since operation of the WCCL began, indicating an improvement in overall water quality. Surface water temperature in the cooling lake during spring and summer periods has been warmer than in preoperation years (particularly Location 2) as is expected due to plant operation.

There appears to be a slight trend of increasing concentrations of iron, chromium, copper and sulfate in the cooling lake; however, this trend does not appear to indicate adverse impact from plant operations but rather natural changes in impounded water.2.4.3 Groundwater Studies Groundwater data collected near WCGS since 1973 have shown that quality of well water varied widely among wells. Data collected during 1987 indicated water quality parameters from the monitoring wells were within concentration ranges observed in previous studies with few exceptions; some dissolved constituents 2-15 (Cl, Mg, and Fe) were lower in one or more wells in 1987 than in previous years. Well water at the monitoring sites has typically been very hard with high levels of dissolved constituents.

Water quality in the wells tends to reflect shallow perched water resulting from precipitation and runoff. These observations have not changed since dam closure for the WCCL or after WCGS began operation.

2-16

2.5 REFERENCES

American Public Health Association, Pollution Control Federation.

of Water and Wastewater.

13th American Public Health Association, Pollution Control Federation.

of Water and Wastewater.

14th American Public Health Association, Pollution Control Federation.

of Water and Wastewater.

15th American Water Works Associations and Water 1971. Standard Methods for the Examination Edition. APHA, Washington.

1134 pp.American Water Works Associations and Water 1975. Standard Methods for the Examination Edition. APHA, Washington.

1134 pp.American Water Works Associations and Water 1980. Standard Methods for the Examination Edition. APHA, Washington.

1193 pp.EA Engineering, Science and Technology, Inc. 1985. Gerald Gentleman Station Aquatic Ecology Study Sutherland Reservoir 1984 Annual Report. Report to Nebraska Public Power District, Columbus, Nebraska.Ukpaka, S.O. 1971. A Eutrophication and Water Quality Study of Five Flood Control Reservoirs.

A thesis presented to the faculty of the Graduate College at the University of Nebraska.

Lincoln, Nebraska.U.S. Environmental Protection Agency. 1979. Methods for Chemical Analysis of Water and Wastes. Environmental Monitoring and Support Laboratory, Cincinnati.

2-17 Spatial Total Suspended Solids Neosho River Spatial Total WCGS Suspended Solids Cooling Lake 2501 200-S150-100-50-0 Loc 1 MLoc 10 Loc 4~bL 42861-Loc 2 Loc 6= Loc 8 hl i 0 1974 1976 1978 1980 1982 1984 1986 1981 1982 1983 1984 1985 1986 1987 I0 Annual Total Suspended Neosho River Solids Annual Total Suspended WCGS Cooling Lake Solids wi U)E 25Cr 2M0 150-100-50-0 w U)E 150-125 100-75-50-25-0 1974 1976 1978 1980 1982 1984 1986 S 1 9 8 11 1' 1 1 1981 19ý82 1983 1984 1985 1986 1987 Figure 2-1.Total suspended solids In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

Spatial Calcium Concentrations Neosho River M Loc 1-I Ii I I 100-90-80-70" 6 50 30 20 1 Spatial Calcium Concentrations WCGS Cooling Lake Loc 2 MILoc 6 Loc 8 I--.1974 1976 1978 1980 1982 1984 1986 Annual Calcium Concentrations Neosho River 0 1981 1982 1983 1984 1985 1986 1987 Annual Calcium Concentrations WCGS Cooling Lake 100-w 90" cn 80-~70-41 60-5 0-30" 20-10-M--, 100-LuJ 90" U) *80-70-41 60-120-10-0 19474 19476 ' 1978 ' 1980 ' 1982 ' 1984 ' 1986'1981 1982 1983 1984 1985 1986 1987 Figure 2-2. CalciLrn concentrations In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

Spatial Magnesium Concentrations Neosho River I I I I 25 20 15 1 Spatial Magnesium Concentrations WCGS Cooling Lake Loc 2 M Loc 6 EM Loc 8 0 0 0, 0 1974 1976 1978 1980 1982 1984 1986 Annual Magnesium Concentrations Neosho River 1981 1982 1983 1984 1985 1986 1987 Annual Magnesium Concentrations WCGS Cooling Lake w Co A 44 I I 25-20-15-10-w 41 Il.I 25-20-15-10-5-5-0 I I I I I I I I I I I I 198 1974 1976 1978 1980 1982 1984 1986 1981 1982 1983 1984 1985 19 86 1987 Figure 2-3. Magneslm concentrations In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

Spatial Orthophosphate Concentrations Neosho River Spatial Orthophosphate Concentrations WCGS Cooling Lake M Loc 1 EM Loc 10 M Loc 4 a I 0.10-o.o9-0.08.0.07--0.06" 0.05-0.04" 0.03-0.02-0.01.0.00 M Loc 2 no Loc 6 M Loc 8 I h wHM01 IF 1974 1976 1978 1980 1982 1984 1986 Annual Orthophosphate Concentrations Neosho River 1981 1982 1983 1984 1985 1986 1987 Annual Orthophosphate Concentrations WCGS Cooling Lake 02 U)0.25-0.20-0.15-0.10-0.05-0.10-LL 0.09-U)0.08-0.06-0.05-'0.04-0.03-0.01-0.00 2-4ý0.00 I ' 1476 'I I I I ' 1 B 1974 197"/6 '1978 1980'1982 19184 19=88 19181 1982 19183 1984 1985 19186 1987 Figure 2-4. Soluble orthophosphate concentrations In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

-r, -F.Spatial Total Iron Concentrations Neosho River Spatial Total Iron Concentrations WCGS Cooling Lake I I I 5.0-4.0-3.0-2.0-1.0-0.0 M Loc 2 Loc 6 Loc 8 L-~ -U -~ .~ L 0.0 0100 w- """' -Ino I.N.)1974 1976 1978 1980 1982 1984 1986 Annual Total Iron Concentrations Neosho River 1981 1982 1983 1984 1985 1986 1987 Annual Total WCGS Iron Concentrations Cooling Lake uJ (n 5.0-4.0-3.0-2.0-w 4.0-41 3.0-0.0 0.0 r I I I I I I 1 1 1974 1976 1978 1980 1982 1 198a4' 19r86 'Figure 2-5. Total Iron concentrations In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

Spatial Total Alkalinity Neosho River Spatial Total Alkalinity WCGS Cooling Lake ii I 0 1974 1976 1978 1980 1982 1984 1986 Annual Total Alkalinity Neosho River 300-w U)250 200ýý150-0 300-1981 1982 1983 1984 1988 1986 1987 Annual Total Alkalinity WCGS Cooling Lake w U)250-41200-50-0 I I I I I I I I I I i 1 I 1974 1976 1978 1980 1982 1984 1986 1981 1982 1983 19;84 1985 1986 1987 Figure 2-6. Total alkalinity concentations In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

Spatial Nitrate Concentrations Neosho River Spatial Nitrate Concentrations WCGS Cooling Lake 2.5-2.0 1.5 0.5-0.0 MLoc I 5M Loc 10 LOC 4-Loc 2 Loc 6 Loc 8 I I 1974 1976 1978 1980 1982 1984 1986 Annual Nitrate Concentrations Neosho River 1981 1982 1983 1984 1985 19B6 1987 Annual Nitrate Concentrations WCGS Cooling Lake 2.5-w U)1.5-1.0 0.5-w U)41 I KVV 0.0 1974 1976 1978 1980 19'82 ' 1984 ' 1986 1987 Figure 2-7.Nibtate concentratlons In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

Spatial Total Disolved Solids Neosho River Spatial Total Disolved Solids WCGS Cooling Lake M Loc 2Loc 6__Loc 8 I I I I 1974 1976 1978 1980 1982. 1984 1986 U,)Annual Total Dissolved Solids Neosho River 1981 1982 1983 1984 1985 1986 1987 Annual Total Dissolved Solids WCGS Cooling Lake w (0 A 4'I 500-400 300o 200 100 0 50(0 w 400-300-f200 10 1974 1976 1978 1980 1982 1984 1986 v 1981 1982 1983 1984 1985 1986 1987 Figure 2-8. Total dissolved solids In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

Spatial Sulfate Concentrations Neosho River I I 100 9O0-80: 70-50-30: 20-10-0 Spatial Sulfate Concentrations WCGS Cooling Lake MLoc 2 EM Loc 6 M-Loc 8 1974 1976 1978 1980 1982 1984 1986 1981 1982 1983 1984 1985 1986 1987 Annual Sulfate Concentrations Neosho River Annual Sulfate Concentrations WCGS Cooling Lake w C')100-90-80-70-60-50-40-30-20-10-w 41 100" go-80-70-60-50-40-30-20-10-0 0'I I I I I I I I ~1974 19176 ' 1978 19480 1982 I 194 ' 1986 'I I I I I I I 1981 1982 1983 1984 1985 1986 1987 Figure 2-9.Sulfate concentrations In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

2.0 1.5-1.0.5-0.0 Spatial Total Organic Nitrogen Neosho RiverLoc 10 M22 Loc 4 19 h1 NIu I I I 2.5-2.0 1.5 0.5 0.0 Spatial Total Organic Nitrogen WCCL Cooling Lake MLoc 2 EM] Loc 6 10 Loc 8 1974 1976 1978 1980 1982 1984 1986 1981 1982 1983 1984 1985 1986 1987 Annual Total Organic Neosho River Nitrogen Annual Total Organic Nitrogen WCGS Cooling Lake w w)A-H 2.5-1.5-1.0-0.5-(I)41 2Z5-2.0-1.5-1.0-0.5-0.0 0.0'1974 1976 1978 1980 1982 1984 1986 I9I1 I I 1981 1982 1983 1984 1985 1986 1987 Figure 2-10. Total organic nifrogen concentratlons In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

Spatial Nickel Concentrations Neosho River Spatial Nickel Concentrations WCGS Cooling Lake 50-I 30 20 10 0-Loc I M Loc 10Loc 4 1974 1976 1978 1980 1982 1984 1986= Loc 8 M Loc 2 I MLoc 6~LE 0 1981 1982 1983 1984 1985 1986 1987 00 NJ Annual Nickel Concentrations Neosho River Annual Nickel Concentrations WCGS Cooling Lake 50-w U)A 44-j I 30-20-10-wu U)A 41 25-20 10 01 I 1 47 178 1 0 1 1 1 1 4 1 1 1974 1976 1978 1980 1982 1984 1985 1981 1982 1983 1984 1985 1986 1987 Figure 2-11. Nickel concentrations In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

Spatial Ammonia Concentrations Neosho River I I I I 025-02O 0.15'0.11 0.05 Spatial Ammonia Concentrations WCGS Cooling Lake-Loc 2 L0oc 6 Loc 8 I 111K 0.00 0.00 1981 1982 1983 1984 1985 1986 1987 1974 1976 1978 1980 1982 1984 1986 Annual Ammonia Concentrations Neosho River Annual Ammonia Concentrations WCGS Cooling Lake 0.251 w U)0.20 0.15-0.10 0.05 0.001 0.25-w A 020-0.15-i 0.1 0-0.00 1 7 I I I I I I I I 19 1974 1976 1978 1980 1982 1984 1986 I I I I I 1 1 1981 1982 1983 1984 1985 1986 1987 Figure 2 Arrnonla concentrations in the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

I I 100 7(>60-50-40-30" 20 I Spatial Chemical Oxygen Demand Neosho River 1 Loc I M Loc 10 Loc 4 dnnhI 4 I hA Spatial Chemical Oxygen Demand WCGS Cooling Lake I U 1974 1976 1978 1980 1982 1984 1986 N3 0 1981 1982 1983 1984 1985 1986 1987 Annual Chemical Oxygen Demand WCGS Cooling Lake Annual Chemical Oxygen Neosho River Demand 100-IM 0, 80-A 7 ('41 60-J40, 30-10, 0 U'(I)A*0I I I so-40-30-20-10-0 1974 1976 1978 1980 1982 1984 1986 I I I I 1 1 1981 1982 1983 1984 1985 1986 1987 Figure 2-13.Chemical oxygen demand In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

Spatial Biochemical Oxygen Demand Neosho River I Spatial Biochemical Oxygen Demand WCGS Cooling Lake 5.0 MLoc 2 4.E Loc 6 017- Loc 8 12.0 1.0 0.0t -I 1981 1982 1983 1984 1985 1986 1987 Annual Biochemical Oxygen Demand WCGS Cooling Lake 1974 1976 1978 1980 1982 1984 1986 I-.Annual Biochemical Oxygen Demand Neosho River 5.0" 41~3a0-i 1.104.0 5.0-40ý.3o-2.0-0.0I llll -v.v I T I 7T I I 1 76I I I 1974 1976 1978 1980 1982 1984 1986 1981 1982 1983 1984 1985 1986 1987 Figure 2-14. Biochemical oxygen demand In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

I:1 50 45-4O-35-30-25-2O-15-5 Spatial Copper Concentrations Neosho River Loc I EMI Loc 10[M Loc 4 1974 1976 1978 1980 1982 1984 1986 10.0-9.8.O-4.0 2.0 1.0 0.0 M Loc 2 LOC 6 Loc 8 Spatial Copper Concentrations WCGS Cooling Lake 1981 1982 1983 1984 1985 1986 1987 iA Annual Copper Concentrations Neosho River Annual Copper Concentrations WCGS Cooling Lake 50 w U)A 4 443 30o 20, 10.0-wU 9.0-8.0-41 6.0-5 .0-ao-12.0-1.0-1974 1976 1978 1980 1982 1984 1986 vv I 1 l 1 I I 1981 1982 1983 1984 198B5 1986, 1987 Figure 2-15.Copper concentrations In the Neosho River and WCGS Cooling Lake near Wolf Creek Generating Station.

Spatial Chromium Concentrations Neosho River Spatial Chromium Concentrations WCGS Cooling Lake MLoc I M Loc 10 EM Loc 4 I I I I 50-40-30-20-10-0-1 Loc 2 Loc 6 l l Loc 8.- mI 1974 1976 1978 1980 1982 1984 1986 1981 1982 1983 1984 1985 1986 1987 Annual Chromium Neosho Concentrations River Annual Chromium Concentrations WCGS Cooling Lake 50 w C,)40-30-3o: w 41 I 0 Id I I I I I I I I I I I I I I 1974 1976 1978 1980 1982 1'984 1986 Figure 2-16. Chromiun car Cooling Lake 1981 1982 1983 1984 1985 1986 1987 rcentrations In the Neosho River and WCGS near Wolf Creek Generating Station.

Location 2 Surface Temperatures Location 6 Surface Temperatures S 5 I I-0 S S I I-0 APR JUN AUG OCT DEC I.'-'S APR JUN AUG OCT DEC Average Lake Surface Temperatures Location 8 Surface Temperatures EM Pre-O-pr.(1984-85)Opemtlon (1985-87)S S S S I 0 APR JUN AUG OCT DEC APR JUN AUG OCT DEC Figure 2-17.Surface water temperatures In WCGS Cooling Lake near Wolf Creek Generating Station.

30-3 I I 28'22-1Is 14-10 Location 6 -1985-Apr un - 4Jil A-'- Aug --&- Oct-A -A -A -A -A A

A -A -A -A &A* Aý0 Location 6 -1986-~ Apr -G- Jun + -U-I A- -Aug-0-- Oct i3 S I I 0 5 10 15 20 25 0 5 10 15 20 25! Depth (m)Depth (ml S I.-I I 30'26 22 18i 14'10, 6 Location 6 -1987--- Ap --G- Ju -I-- Aug -.A, Nov k " A. IA- A- A, A .A-A A- A- A- -A -A -.-. A -A A -A- A Figure 2-18.Depth profiles of water terrperature at Location 6 In WCGS Cooling Lake near Wolf Creek Generating Station, 1985-1987.

0 5 10 15 20 25 Depth (m)

C'. 7, Location 6 -1985 A Apr -G- Jun -Jul A"- Aug 0 Oct c~.16-14-12-10-a-6-4" 2" nýLocation 6 -1986 Apr -- Jun -+-- Jul -A. Aug --- Oct 16-~tXt&A.A-A-A-A.A.A A A-I 12-10-8" 6-41 2-0 0 A, I,-A.'AL.I "'-A.Ik A 0 0 5 10-T- ..--I~15 20 25 5 10 15 Depth (Im)20 25 t..I'Depth (m)Location 6 -1987 6 Apr --G- Jun ---- Aug" A'A Nov 16-14-12-10-a-6-4-2-0 0~'A" A " A , A A. A. A .A A. A .A, A A. A .A .A. A Figure 2-19. Depth profiles of dissolved oxygen at Location 6 in WCGS Cooling Lake near Wolf Creek Generating Station, 1985-1987.

0~Q-G-G-G-O 10 15 Depth (m)20 26 TABLE 2-1

SUMMARY

OF LOCATIONS SAMPLED AT WOLF 1974-1987 CREEK GENERATING STATION, River and Creek Locations Lake Locations Year 1974 1975 1976 1977 1978 1979 1980 1981 1982 1983 1984 1985 1986 1987 I(a)3 4 5 7 10 x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x K x x x x K K K K K K K x 2(a)K x x x K K x x x 6 x x x x x x x 8 x x x x (a) Location 1 -Location 2 -present.Above John Redmond Dam 1974-1975.

Upper Wolf Creek before dam closure-in upper lake 1981 to?-37 TABLE 2-2 WELLS SAMPLED AS PART OF GROUNDWATER PROGRAM NEAR WOLF CREEK GENERATING STATION, 1974-1987 Well #B-12 C-6 C-10 C-20 C-49 C-50 D-24 D-26 D-28 D-42 D-55 D-65 74 x x 75 x x x x x x x 76 x x x x x 77 x x x x x x 78 x x x x x x 79 x x x x x X x 80 x x 81 x X X 82 X x x 83 X X x 84 x X X 85 x X X 86 X X x 87 X X X-X X X K K K K K K X K K X Note: X indicates well was sampled during the year.2-38 TABLE 2-3

SUMMARY

OF PARAMETERS ANALYZED IN GROUNDWATER SAMPLES FROM WELLS NEAR WOLF CREEK GENERATING STATION, 1974-1987 Parameter 74 75 76 77 78 79 80 81 82 83 84 85 86 87 Alkalinity X X x X X X X x K K X x X X Calcium X X X X X x x X X X X X X X Magnesium X X x X X X X X X X X K X X Potassium X x X X X X X x ----.Sodium X X x X X X X X ----.Chloride X X x X X x X X X X X X X X Sulfate X X x X X X X X X X X X x X TDS X X x X X X X x X X x X X X Conductance X X X X X x x x X X x X X X Nitrate x x x x X X -X X X X X x X Phosphorus X X X X X X K K K -----Silica X X X X X X K K K -----Iron (Total) X X X X x X X x X X x X X x Iron (Soluble)

-X X X X X X X X X X x X X Manganese X X X X X X X K K K K ---Manganese

-X ........... .pH X x x X X X X X x X X x X X Hardness ......x -X x X X X X NJ Selenium --K K K K K K X .....I TABLE 2-4 NUMBER OF ANALYSES CONDUCTED ON SURFACE WATER QUALITY SAMPLES COLLECTED NEAR WOLF CREEK GENERATING STATION, 1974-1987 0 Parameters Oil and grease Water temp Dissolved oxygen Oxygen saturation pH Alkalinity Sulfate TDS Specific Cond.TSS Turbidity Color, True Ammonia Nitrate Nitrite Organic Nitrogen Orthophosphate Sol.Phosphorus Silica Bact. Fecal Colif.BOD COD Organic Carbon Hexane Sol.Copper, Total Iron, Total Lead, Total Manganese, Total Mercury, Total Zinc Calcium Chloride Magnesium Potassium Sodium Iron (sol.)Bact. Fecal Strep.Selenium Hardness Chromium, Total Nickel--------24 48 60 22 24 20 20 16 16 12 24 26 48 60 24 24 36 39 32 35 23 48 47 48 56 22 24 36 39 28 31 48 56 24 24 36 36 36 36 24 48 48 48 60 24 24 36 36 36 -24 46 48 48 60 24 24 36 36 36 36 24 48 48 48 60 24 24 36 36 36 36 24 46 48 48 60 24 24 36 36 36 36 18 46 48 48 54 24 24 36 36 36 36 24 47 48 48 60 24 24 36 36 36 36 24 48 48 48 60 24 24 36 36 36 36 18 48 48 --24 24 36 36 36 36 24 45 48 48 60 22 24 36 36 36 36 24 48 24 48 60 24 24 36 36 36 36 24 48 24 48 60 24 24 36 36 36 36 24 48 48 48 60 24 24 36 36 36 36 24 48 24 48 59 24 24 36 36 36 36 24 48 48 --24 24 36 36 36 36 24 47 30 --20 22 36 34 35 35 24 48 -48 60 24 24 36 36 36 35 24 48 48 47 59 24 24 36 36 36 36 24 48 48 48 60 24 24 36 36 36 36 24 48 48 --24 20 35 31 34 33 21 44 ---24 24 36 36 36 36 24 48 48 38 60 24 24 36 36 36 36 24 48 48 48 60 24 24 36 30 36 36 18 48 48 --24 24 36 36 36 36 24 48 24 48 60 24 24 30 36 36 36 24 48 ---24 24 36 36 36 35 24 48 48 ----36 36 36 36 24 48 24 48 60--36 36 36 36 24 48 48 48 60--36 36 36 36 24 48 24 48 60--36 36 36 36 24 48 24 ----36 36 36 36 24 48 24 ----36 36 36 36 24 48 24 48 60--24 33 36 35 24 23 -----36 36 36 36 18 48 ---........48 --........- 48 60........- 48 60 71 36 51 63 72 72 72 72 72 72 72 72 72 72 72 72 72 72 72 72 72 72 72 72 71 72 72 72 47 33 22 57 36 33 22 72 48 72 48 72 48 71 48 72 48 71 48 72 48 72 48 72 48 72 48 72 48 66 48 72 48 72 48 64 48 72 48 72 48 72 46 72 48 72 48 72 48 72 48 72 48 1974 1975 1976 1977 1978 1979 1980 1981 1982 1983 1984 1985 1986 1987 Note: Total analyses scheduled for collections from Neosho River, Wolf Creek, and WCCL.

TABLE 2-5 ANNUAL MEAN CONCENTRATIONS OF SELECTED WATER QUALITY PARAMETERS FOR THE COOLING LAKE (WCCL) NEAR WOLF-CREEK GENERATING STATION, 1974-1987 NEOSHO RIVER AND WOLF CREEK Parameter Bacteria-Fecal coliform Bacteria-Fecal streptococcus Chloride Color Dissolved oxygen Iron-soluble Lead Manganese Mercury Nitrite Oil and Grease pH Phosphate-Total Potassium Selenium Silica Sodium Specific Conductance Total organic Carbon Turbidity Water Temperature Zinc Parameter Bacteria-Fecal Coliform Bacteria-Fecal Steptecoccus Chloride Color Dissolved oxygen Iron-soluble Lead Manganese Mercury Nitrite Oil and Grease pH Phosphate-Total Potasium Selenium Silica Sodium Specific Conductance Total Organic Carbon Turbidity Water Temperature zinc 1974 75 76 77 78 79 Neosho River 80 81 82 83 84 85 86 87 610 16 11.1 7 0.08 0.1 0.03 0.6 7.9 0.14 6.6 456 13 35 12.4 21.5 148 19 9.1<3.09 2.0 0.02 1.4 7.7 0.15 4.4 468 18 64 14.7 15.2 146 104 26 11 9.3 0.10 (2 0.12 (6.5 0.02 0.48 8.1 0.21 4.2 3.8 3.0 22 592 7.6 27 15.2 8.7 100 53 34 22 9.8 0.09<3.7 0.12<1.1 0.02 (3 8.0 0.17 4.9<2 6.9 21 552 8.2 43 14.3 17.5 194 43 31 12 10.0 0.06 9.4 0.09 1.1 0.02 2.5 8.1 0.13 4.9 2.8 3.7 25 620 8.0 27 11.9 16 602 160 23 15 9.9 0.06<3 0.09 0.65 0.02<3 8.1 0.15 5.3 21 4.5 19 501 8.6 35 13.2 23 278 80 22 21 10.6 0.16<2.0 0.11<1 .9 0.02 51 7.8 0.17 4.8<1.7 1.4 20 477 8.0 29 11.2 32 343 29 8 9.6 0.27<4.3 0.11<4.8< .02<19 7.9 0.33 4.4<0.7 07.3 20 399 6.4 30 15.6 13 9.9 0.15<3.9 0.06 0.03 7.9 3.9 437 7.8 35 15.1>200* 44 18 13 10.6 10.5<0.12 <0.86 0.11 0.17 (0.02 0.05<3.2 <3 7.9 7.8 478 413 27 54 12.1 11.0>68 9 9.5<0.13 0.06<3 7.5 361 56 14.7 19 11 11.2 0.08<0.03 (3 7.6 396 33 14.0 45 11 9.7 0.05 0.05 (3 7.7 417 44 20 (50 <11.1 <21.3 WCCL 1981 3 80 26 4.8 9.4 0.30<2.7 0.08<2.1<0.01<10.3 7.9<0.15 4.2<0.5 1.8 19 436 6.0 13.5 15.9<16.4 82 83-12 15 15 4.6 -9.4 8 .9<0.15 <0.5-0.07<0 .03<0.01<1.1 (3.1 7.8 7.9<0.26 -4.1 -84 5 14 8.7<0.005 0.07<0.01<3.0 1.8 85 2 13 9.7<0.08 86 12 12.8<0.09<0.01 <0.01<3.1 <3.0 7.6 7.7 87 4 16 8.9<0.1<0.01<3 8.0 353 5.2 22.2<2.4 11 407 6.9 5.3 14.7:28.8 376 6.0 14.7 367 4.2 15.1 367 5.8 16.5 319 4.2 15.7 Note: Dash (-) indicates the parameter was not scheduled for determination.

TABLE 2-6 MEAN CONCENTRATIONS OF WATER QUALITY PARAMETERS FOR WELLS SAMPLED NEAR WOLF CREEK GENERATING STATION, 1977-1987 Well B-12 Units 1977 1978 1979 1980 1981 1982 1983 1984 1985 1986 1987 pH units 7.3 7.6 7.6 7.3 6.8 7.6 8.2 7.6 8.3 8.2 7.5 Alkalinity mg/1 365 390 290 259 401 572 451 491 486 774 522 Specific conductance pmhos/cm 1,155 1,200 1,100 635 2,140 3,196 2,850 1,770 1,615 985 2,100 Hardness mg/l ---909 -1,301 883 642 386 400 677 Total dissolved solids mg/l 759 793 757 690 2,283 2,415 2,113 970 1,212 1,003 1,479 Calcium mg/l 107 116 115 260 398 262 232 62 108 111 196 Chloride mg/i 60 81 97 57 372 269 238 194 126 75 342 Magnesium mg/i 22.6 21.3 22 65 94 25.9 73.8 131 39.6 38.6 45.6 Sulfate mg/l 117 170 220 387 450 620 484 413 227 120 48 Nitrate mg/l 10 4.2 54.6 81 21.5 2.2 <.05 114 Iron, total mg/l 1.8 3.6 1.5 16.4 32 "20 20.4 20.0 28.4 56.3 5.4 Iron, soluble mg/1 0.26 0.08 0.01 0.80 0.03 0.15 0.87 16.5 0.14 0.24 0.12 Well C-20 Units 1977 1978 1979 1980 1981 1982 1983 1984 1985 1986 1987 pH units 7.1 7.5 7.1 6.8 -7.0 7.4 7.6 7.3 7.1 7.3 Alkalinity mg/l 262 249 186 224 -292 258 312 273 295 579 Specific conductance pmhos/cm 1,370 1,600 1,400 1,300 -1,295 1,665 1,152 1,572 1,169 1,100 Hardness mg/I ---702 -788 790 650 655 545 653 Total dissolved solids mg/i 1,090 1,192 1,140 654 -952 1,126 556 1,091 1 084 985 Calcium mg/l 200 217 220 250 -300 280 102 224 241 222 Chloride mg/i 170 147 130 150 -128 188 121 284 135 243 Magnesium mg/i 21 20 22 19 -14.2 22.6 20.5 24.2 15.0 23.9 Sulfate mg/l 45 32 60 --53 49 68 166 50 53 Nitrate mg/l 61 -29.9 78 31.4 42.2 50.3 50.1 Iron, total mg/l 1.8 0.9 1.6 5.8 -15.1 1.5 3.5 7.0 <1.6 2.4 Iron, soluble mg/l 0.12 0.03 0.08 0.05 -1.4 0.12 3.4 0.18 <0.03 0.07 Well D-65 Units 1977 1978 1979 1980 1981 1982 1983 1984 1985 1986 1987 pH units 6.9 7.3 7.5 6.5 7.5 6.8 6.8 7.0 7.2 6.9 7.1 Alkalinity mg/l 161 130 158 110 190 167 138 164 150 153 320 Specific conductance vmhos/cm 5,093 5,530 5,700 4,700 3,040 4,399 4,031 3,040 3,625 2,592 2,000 Hardness mg/1 ---2,070 -1,634 1,379 1,294 1,412 1,250 868 Total dissolved solids mg/i 3,858 4,690 4,565 1,162 2,914 3,146 4,342 1,774 2,954 2,747 2,229 Calcium mg/1 499 596 750 690 501 498 394 184 416 380 270 Chloride mg/l 497 510 610 6,045 576 578 352 459 386 312 142 Magnesium mg/l 120 133 150 114 96 74 96 65 77 90 47 Sulfate mg/l 20 <1 180 30 44 62 74 60 58 68 59 Nitrate mg/i 0.45 -0.70 -1.4 310 372 178 231 173 171 Iron, total mg/i 10.9 7.8 6.1 4.3 4.6 31.5 26.9 65 12.0 6.7 9.0 Iron, soluble mg/1 0.027 0.033 0.011 0.12 2.66 5.57 0.45 17.5 0.29 (0.05 0.06 TABLE 2-6 Cont.Well C-49 Units 1980 1981 1982 1983 1984 1985 1986 1987 pH units 7.0 7.1 7.0 7.2 7.0 7.4 7.3 7.1 Alkalinity mg/i 265 348 431 368 356 348 376 541 Specific conductance vmhos/cm 1,100 860 1,160 1,348 1,314 1,191 1,026 1,000 Hardness mg/i 499 -530 518 633 515 461 477 Total dissolved solids mg/1 742 781 785 1,418 730 800 792 841 Calcium mg/i 140 126 126 143 64 141 206 125 Chloride mg/i 3,066 84 48 72 44 74 47 52 Magnesium mg/i 37 31 405 39 36 36 34 40 Sulfate mg/1 151 188 206 258 278 143 114 46 Nitrate mg/i -3.4 11.5 186 8 6.6 63.7 4.4 Iron, total mg/1 -0.24 0.10 0.09 0.18 0.09 3.8 0.07 Iron, soluble mg/1 0.068 0.23 0.14 0.06 (0.1 0.08 <0.03 0.32 I,

3. PLANKTON PRODUCTIVITY

3.1 INTRODUCTION

The plankton of freshwater lakes and rivers is composed of small plants and animals. Their position both vertically and horizontally in the water column is for the most part not self-controlled but rather is determined by physical factors such as currents, eddies, and turbulence.

Phytoplankton consists of various types of algae, microscopic plants, which are suspended in the water column. While some algal taxa normally occur in suspension, others are washed free from substrates to which they were attached.

Phytoplankton is given consideration in aquatic studies because it forms the primary trophic level of the aquatic food chain. Several species of zooplankton and macroinvertebrates feed on phytoplankton (Jonasson 1969, Saunders 1969), as do some fish species such as gizzard shad (Cramer and Marzolf 1970). There are also aesthetic considerations which may affect recreational use of lakes and reservoirs.

Large algal blooms may occur sporadically during warm weather, cloud the water, and make recreational activities such as fishing, swimming, and boating less enjoyable.

Zooplankton are generally considered the second link (primary consumers) in aquatic food chains, although certain common limnetic taxa such as Cyclops species prey upon other zooplankters and thus are regarded as secondary consumers (Fryer 1957, McQueen 1969). Zooplankton that are primary consumers feed on phytoplankton, bacteria, protozoa, and organic detritus and are in turn utilized by aquatic macroinvertebrates and fish (secondary and tertiary consumers).

Most fish species at some stage of development depend primarily on 3-1 zooplankton as a food source. Availability of sufficient quantities of zooplankton at critical periods may affect survival of the fry and fingerling stages of fish species that are of commercial or recreational value (Siefert 1972, Clady 1977). Gizzard shad, the primary species of forage fish in the diets of walleye, white bass, and largemouth bass in several midwestern reservoirs, relies exclusively on zooplankton during a portion of its early life history (Kutkuhn 1957, Cramer and Marzolf 1970).Plankton studies at WCGS began on the Neosho River in 1973 and on the Wolf Creek Cooling Lake (WCCL) in 1981. Although these studies at one time included determinations of phytoplankton and zooplankton composition and abundance, for several years emphasis has been placed on standing crop and productivity estimates for WCCL. These include phytoplankton chlorophyll a concentrations, phytoplankton carbon fixation rates, zooplankton dry weight biomass, and zooplankton ash-free dry weight biomass. Although plankton studies in the river were discontinued in 1982, phytoplankton studies resumed the next year, and measurements of chlorophyll a concentrations continue.

The primary purpose of the present study is to examine spatial and temporal trends in the plankton data for effects of WCGS operation, which began in August 1985.3.2 METHODS Phytoplankton samples were collected from the Neosho River either quarterly or bimonthly from 1973 through 1987, except in 1982 when sampling was not conducted.

In all years sampling occurred at Location 1 either in John Redmond Reservoir or its tailwaters, at Location 10 upstream of the confluence with Wolf Creek, and at Location 4 downstream of Wolf Creek (Figure 1-1). At each 3-2 location whole water was collected for six replicate determinations of chlorophyll a concentration and carbon fixation rate. Bimonthly plankton sampling in WCCL began in 1981 at Location 2 near the WCGS discharge and Location 6 near the dam. Location 8 near the WCGS intake was added to the program in 1984. As in the river, phytoplankton collections consisted of surface sampling of-whole water for six replicates at each location.Zooplankton biomass sampling consisted of four replicate vertical tows (bottom to surface) with a 30-cm diameter, No. 10 (153 jim) mesh conical plankton net.Analytical methods for the plankton standing crop and productivity measurements are described in detail in recent WCGS annual reports. For phytoplankton, chlorophyll a concentrations (corrected for phaeophytin) were determined fluorometrically from acetone extracts (Lorenzen 1966; Strickland and Parsons 1972; APHA 1981) and were reported per cubic meter of water (mg/m3 ). The rate of carbon fixation was estimated with the light bottle/dark bottle C-14 method (Wetzel 1964; Parkos et al. 1969; Strickland and Parsons 1972), and primary productivity was expressed as carbon fixed per cubic meter per hour (mg/m3 /hr).Zooplankton biomass was determined gravimetrically after drying at 60 C for dry weights (Lovegrove 1962) and after incineration at 500 C for ash-free dry weights (APRA 1981). Both zooplankton biomass parameters were reported per cubic meter of water (mg/m3 ).Overall spatial trends in the data were examined by plotting average annual values for each location in each year of sampling.

Three approaches were used to evaluate temporal trends. First, average values for either the river or lake were calculated for each sampling date. The resulting plots permitted detailed examination of temporal cycles within each year. Secondly, average 3-3 bimonthly (seasonal) values were calculated for each of three time periods.For the river these periods were prior to lake-construction and dam closure on Wolf Creek (Pre-Lake, 1973-1979), Pre-Operational (1980-AUG 1985), and WCGS operational (OCT 1985-1987).

For the cooling lake, the Pre-Lake period was replaced by Lake Filling (1981). This approach allowed conditions during station operation to be compared with those of earlier periods. Finally, annual means and standard errors were calculated to examine overall year-to-year trends in the data.3.3 RESULTS AND DISCUSSION 3.3.1 Neosho River Phytoplankton Chlorophyll a concentrations in the Neosho River ranged from <1 to nearly 144 mg/m3 (Tables 3-1, 3-2). Spatially, annual phytoplankton standing crop was often greatest at Location 1 and usually very similar at Locations 10 and 4 (Figure 3-1). The phytoplankton of the river is strongly influenced by releases from John Redmond Reservoir, which constitute nearly all of the flow in this section of the river. During moderate to high flows, chlorophyll concentrations immediately upstream and downstream of the confluence with Wolf Creek were very similar to those observed in the tailwaters.

During low flow conditions, values at Location 10 immediately upstream of Wolf Creek were often different (usually but not always higher) than those observed at the other locations.

Temporally, no consistent pattern was noted in standing crop for the individual sampling dates (Figure 3-1). Unusually high values (>100 mg/m3 ) occurred in 3-4 February 1984 and March 1986. On a seasonal basis, little variability was noted in chlorophyll for the period 1973 through 1979. Although there was a tendency for slightly lower values in June and higher values in late summer, average chlorophyll fell within a relatively narrow range (= 10-20 mg/m3 ).During the 1980 to 1985 preoperational period, standing crop was distinctly higher in late winter with a minor peak evident in late summer. The late winter peak persisted during the operational period when spring and early summer values were higher than in earlier periods. A late summer minimum was also noted during the operational period. On an annual basis, chlorophyll values in the river displayed a moderate increase from 1973 through 1983 and then rose to a maximum in 1984. Since then, annual values have been declining toward earlier levels, except in 1985 when heavy precipitation in the watershed and unusually high river flows resulted in the lowest phytoplankton standing crop observed in the Neosho River.Carbon-fixation rates ranged from 0 to 226 mg C/m 3/hr (Tables 3-1, 3-2). There were no consistent spatial differences in annual productivity, and in most years values were similar at all three river locations (Figure 3-2). There were no apparent seasonal cycles from the temporal results for individual sampling dates, but these data did show that the four greatest productivities all occurred during 1986. These high values caused seasonal means for four of the six seasons during the operational period to be substantially higher than respective values for earlier periods. Not surprising the annual mean productivity was much higher in 1986 than in any other year. However, carbon fixation rate in 1987 was within the range of annual values observed prior to 1986.3-5 The absence of major spatial differences between Locations 10 and 4 in recent years indicated that neither phytoplankton standing crop nor productivity in the Neosho River was affected by WCGS operation.

The general absence of spatial differences prior to WCGS operation suggested that Wolf Creek, which was an intermittent stream, seldom affected river phytoplankton historically.

Instead phytoplankton standing crop primarily reflects releases from John Redmond Reservoir (JRR), with some downstream declines typical for lake plankton subjected to the lotic conditions of a river. Carbon fixation rates in turn are strongly influenced by phytoplankton standing crop as well as natural variations in ambient conditions such as temperature and turbidity.

Thus, temporal patterns for phytoplankton productivity may be modified from those observed for standing crop. The potential for WCGS to impact the Neosho River phytoplankton community has been minimal based on low diversion rates from the JRR tailwaters and the absence of substantial discharges from the cooling lake. When these discharges do occur, any effects on the river phytoplankton would probably be additions to or dilutions of the community that originated in JRR. These effects would be very temporary and only persist for a short time after the discharge was discontinued.

3.3.2 Cooling Lake Phytoplankton Chlorophyll a concentrations in the Wolf Creek Cooling Lake (WCCL) ranged from 1.8 to 25.7 mg/m 3 (Table 3-3). Spatially, standing crop was distinctly greater at Location 2 than at Location 6 through 1984 (Figure 3-3). Since then spatial differences have been less pronounced, probably because WCGS operation has increased the circulation and mixing of waters throughout WCCL. Temporally chlorophyll values tended to be at or near annual maxima in autumn and at 3-6 annual minima in winter or early spring. There has been little evidence of a major spring pulse that is typical of most midwestern lakes and reservoirs.

Unusually high phytoplankton standing crops occurred in October and December 1985 and April 1986, soon after WCGS operation began. Similar peaks did not occur in the JRR tailwaters (Table 3-2), suggesting that factors peculiar to WCCL rather than local climatic factors were responsible.

It is conceivable that increased lake mixing and the onset of thermal discharges associated with VCGS start up caused a temporary stimulation of phytoplankton standing crop.However, chlorophyll values had returned to normal levels by the summer of 1986. The absence of fall peak in 1987 probably resulted because sampling was postponed from October to November.On a seasonal basis, phytoplankton standing crop was consistently highest during lake filling in 1981 (Figure 3-3). This result was not unexpected as the tailwaters of John Redmond Reservoir, a relatively shallow eutrophic reservoir, were the primary source of water for lake filling. Also, the newly flooded soil and vegetation in WCCL would serve as an added source of nutrients stimulating phytoplankton standing crop. More uniform seasonal values were observed during the preoperational period although a fall maximum remained evident. The prominence of the fall maximum continued to decline during WCGS operation.

Other seasons exhibiting progressive declines from lake filling through operation included spring (April) and early winter (December).

On an annual basis, chlorophyll values appeared to stabilize soon after the lake-filling maximum, increased to near lake-fill values in 1985-86, and declined to a 1987 minimum. Most of the annual increases observed for 1985 and 1986 were related to the previously discussed high standing crops recorded soon after WCGS start up. The absence of October sampling in 1987 was a factor 3-7 which reduced that annual mean. Overall annual trends were considered representative of a new lake that was initially filled with eutrophic water and then gradually assumed its own character, with a possible temporary stimulation associated with WCGS start up. Based on average annual chlorophyll a concentrations, the WCGS cooling lake can be classified in the mesotrophic range (Wetzel, 1975).Phytoplankton carbon fixation rates ranged from 0.0 to 115.8 mg C/m3 /hr (Table 3-3). Spatial differences were less pronounced than those observed for chlorophyll a concentrations, but productivity was often greatest at Location 2 (Figure 3-4). Temporally, there were no consistent trends in the timing of annual minima and maxima. Unusually high fixation rates occurred in February 1981, April and October 1982, and April through December 1986. The high productivity in 1986 caused all seasonal values except those in cold months to be higher during the operational period than during earlier periods. However, carbon fixation rates were also unusually high in the JRR tailwaters during 1986, indicating that the cause was not unique to WCCL and not related to WCGS operation.

Different seasonal patterns were evident for each of the three periods examined.

During lake filling in 1981, maximum fixation rates were restricted to cold months (Figure 3-4). In the preoperational period, a bimodal pattern with peaks in April and October was observed.

During WCGS operation, the April peak became dominant and resulted in a unimodal pattern. On an annual basis, phytoplankton productivity has varied between 9 and 17 mg C/m 3/hr since 1982, except for the unusually high value (65 mg C/m3/hr) in 1986. Carbon fixation rates have been strongly influenced by phytoplankton standing crop as 3-8 well as natural variations in ambient conditions (e.g. temperature), and as a result fixation rates have revealed few consistent spatial or temporal trends.Phytoplankton standing crop in WCCL was less than that observed in other regional lakes that are thermally influenced (Table 3-4). Chlorophyll a concentrations in WCCL averaged less than 10 mg/m3 for 1981-87, whereas most other lakes have recorded overall means of 20 mg/mr 3 or greater. Turtle Creek Reservoir, a 1,550-acre Indiana lake, exhibited substantially increased standing crop under two-unit operation but not under one-unit.

Chlorophyll a values in Sutherland Reservoir, a 3,050-acre lake in western Nebraska, peaked during one-unit operation in 1981 and stabilized at somewhat lower values during two-unit operation.

Standing crop in 660-acre Nelson Lake, located in western North Dakota, was similar to that observed in Sutherland Reservoir.

Only Clinton Lake, a 4,890-acre Illinois impoundment, exhibited standing crops considered similar to WCCL, but those values were prior to the onset of thermal enrichment.

Unlike many lakes in midwestern and plains states, the WCCL is deep and does not have a major source of nutrient inputs. These factors may be adequate to prevent eutrophic conditions from developing at WCCL.3.3.3 Cooling Lake Zooplankton Zooplankton biomass in WCCL ranged from 17 to 1,052 mg/m3 for dry weights and from 14 to 338 mg/mi 3 for ash-free dry weights (Table 3-5). Spatial differences in dry weight were minor except in 1985 and 1987 when standing crop was highest at Location 2 and lowest at Location 6 (Figure 3-5). No consistent temporal patterns were evident, but there was a tendency for high dry weights in April and low dry weights in June or August. Unusually high values occurred in 3-9 April, June, and October 1985 and in December 1986. On a seasonal basis, there has been a shift in the occurrence of maximum dry weight from spring in the preoperational period to autumn during WCGS operation.

Based on annual values, zooplankton dry weights declined from 1981 through 1983, stabilized briefly before sharply rising to a 1985 maximum, and then returned to levels similar to the first two years of study. Most of the 1985 maximum was attributable to high standing crops that occurred before WCGS began operation.

Because zooplankton biomass samples sometimes contained sand, silt, and other suspended solids, ash-free dry weights (AFDW) was considered more representative of standing crop. Although AFDW was greater at Location 6 than at Location 2 from 1981 through 1983, there have been no consistent spatial trends in recent years (Figure 3-6). Neither has there been a consistent timing of yearly minima and maxima. Seasonally, standing crop from late winter through summer was greater during lake-filling than in later periods. Vith the exception of early winter, seasonal zooplankton standing crops were similar in preoperational and operational periods. Annually, AFDW declined through 1984, increased through 1986, and again declined in 1987. These annual trends were similar to those observed for dry weights, with the exception of 1985. The nature of differences between dry weight and AFDW in 1985 suggested that dry weights in that year were strongly influenced by non-organic materials (e.g.sand, etc.). The absence of consistent spatial differences in recent years, the similarity in seasonal values for preoperational and operational periods, and the absence of sustained high biomass after WCGS start-up all indicated that WCGS operation was not adversely affecting AFDW standing crop in the cooling lake.3-10 Zooplankton AFDW in the cooling lake has been similar to that observed for lakes Sangchris and Shelbyville in Illinois, but substantially less than that recorded for Nelson Lake, North Dakota (Table 3-4). In WCCL similar annual trends were observed for phytoplankton and zooplankton standing crop (Figure 3-7). However, for both zooplankton parameters there were declines between 1982 and 1983 while chlorophyll a concentrations remained stable. This difference was related to differential declines in plankton production after WCCL was filled and major inputs from John Redmond were discontinued.

Apparently for phytoplankton the decline occurred very soon after lake filling, but for zooplankton it did not occur until June 1982. Because crustacean zooplankters have much longer life cycles than planktonic algae, the degree and extent of these annual zooplankton declines probably reflected relatively slow natural attrition of zooplankton introduced from JRR as well as a response to the declining phytoplankton production.

Other differences between phytoplankton and zooplankton occurred in 1985 for dry weights and in 1986 for AFDW (Figure 3-7). The effect of inorganic materials on the high 1985 dry weights has already been discussed.

Zooplankton AFDW continued to increase in 1986 while phytoplankton chlorophyll a declined slightly.

There was moderately good correlation (r = 0.70) between chlorophyll a concentrations and each zooplankton biomass parameter.

When regression lines were fit to their data, the distribution of points was somewhat better for AFDW because dry weight was affected by the unusually large value for 1985. In the absence of biomass data, chlorophyll a concentrations could be used to estimate zooplankton standing crop.3-11 3.4

SUMMARY

AND CONCLUSIONS 3.4.1 Neosho River Phytoplankton Studies Phytoplankton chlorophyll a concentrations and carbon fixation rates in the Neosho River from the tailwaters of John Redmond Dam to below the confluence with Wolf Creek have been monitored since 1973. Flow in the study area is controlled by releases from John Redmond Reservoir.

During periods of moderate to high flows, chlorophyll concentrations and fixation rates immediately upstream and downstream of the confluence with the creek were very similar to those observed in the tailwaters.

During low flow conditions, values for both parameters immediately upstream of Wolf Creek are often different (usually but not always higher) than those observed at the other locations.

In 1987, both the average annual chlorophyll concentration (27.38 mg/m3 ) and carbon fixation rate (29.86 mg C/m 3 hr) were within the respective ranges (3.81-63.38 mg Chl 3 3 a/m3, 12.18-238.22 mg C/mn hr) observed for previous annual averages.

The 1987 results reflected a return to more normal conditions after the high phytoplankton values resulting from the generally low river flow of 1986.There has been no indication that adverse effects on the phytopiankton of the Neosho River have occurred as a result of the construction and operation of WCGS.3.4.2 WCCL Plankton Studies Phytoplankton chlorophyll a concentrations and carbon fixation rates (surface samples) as well as zooplankton biomass (vertical tows) in the WCGS cooling lake have been monitored bimonthly since initial lake filling in 1981. Average 3-12 annual chlorophyll concentrations declined by approximately 30 percent from 1981 to 1982, remained fairly stable from 1982 through 1984, and returned to near 1981 levels in 1985 and 1986. The annual value in 1987 declined by b 3 approximately 35 percent to 6.6 mg/mr and was below the previous range (7.5-11.0 mg/m3 ) of annual values. Temporally, phytoplankton standing crop has I.I (:i been generally greatest in late-summer or early autumn, and spatially, it has generally been least in the downlake deep water location near the dam.However, exceptions to these general patterns have been observed, and chlorophyll concentrations were unusually high in October and December 1985 and April 1986. Carbon fixation rates have been strongly influenced by phytoplankton standing crop as well as natural variations in ambient conditions (e.g. temperature), and as a result fixation rates have revealed few consistent spatial or temporal trends. Unlike 1986 when unusually high fixation rates were common, the annual mean rate in 1987 (9.1 mg C/mr 3) was slightly below the previously observed range of annual values (11.7-64.4 mg C/m3 /hr).Average annual zooplankton biomass, both dry and ash-free dry weights, declined from 1981 through 1984, although dry weight biomass appeared to stabilize in 1983 and 1984. Ash-free dry weight increased from 40 mg/mr 3 in 1984 to 67 mg/mr 3 in 1985 and 92 mg/mr 3 in 1986, and then declined to 53 mg/mr 3 in 1987. Dry weight peaked in 1985 (234 mg/m3 ) and has since progressively declined in 1986 (154 mg/m3) and 1987 (123 mg/m3 ). Average annual dry weight in 1987 was less than that observed during lake filling in 1981 but greater than the 66 mg/mi 3 minimum of 1984. Few consistent spatial and temporal trends have been observed for zooplankton biomass, but there has been a tendency for greater biomass in the uplake shallower water and for greater biomass in late winter or early spring from 1981-1985 with spring and fall peaks in 1985 and 1986. A spring 3-13 peak also occurred in 1987, but zooplankton sampling was discontinued before the normal period of the fall peak.Annual trends in phytoplankton and zooplankton through 1984 were considered representative of a new lake, with nutrient inputs from recently inundated soil and vegetation, that was initially filled with eutrophic water (from John Redmond Reservoir) and then gradually assumed its own character.

Increases in plankton apparent in 1985 and 1986 were considered primarily a response to natural factors although operational effects of the thermal discharge and altered lake circulation patterns associated with WCGS start-up may have been contributing factors. Plankton declines to normal levels during WCGS operation in 1987 support the conclusion that station operation is not adversely affecting plankton production.

Based on average annual chlorophyll a concentrations, the WCGS cooling lake remains in the mesotrophic classification.

3-14

3.5 REFERENCES

American Public Health Association, American Water Works Association, and Water Pollution Control Federation.

1981. Standard Methods for the Examination of Water and Wastewater.

15th edition. APHA, Washington.

1,134 pp.Clady, M.D. 1977. Crustacean zooplankton populations and concurrent survival of larval yellow perch in Oneida Lake. N.Y. Fish and Game J.24(l):46-52.

Cramer, J.D. and G.R. Marzolf. 1970. Selective predation on zooplankton by gizzard shad. Trans. Amer. Fish. Soc. 99(2):320-332.

Ecological Analysts, Inc. 1981. Entrainment Studies at Gerald Gentleman Station, August 1979 through December 1980. Report to Nebraska Public Power District, Columbus.Ecological Analysts, Inc. 1982a. Gerald Gentleman Station Entrainment Study, 1981 Annual Report. Report to Nebraska Public Power District, Columbus.Ecological Analysts, Inc. 1982b. Wolf Creek Generating Station Construction Environmental Monitoring Program, February 1981 -January 1982. Report to Kansas Gas and Electric Company, Wichita.Ecological Analysts, Inc. 1983a. Gerald Gentleman Station Entrainment Study, 1982 Annual Report. Report to Nebraska Public Power District, Columbus.Ecological Analysts, Inc. 1983b. Wolf Creek Generating Station Construction Environmental Monitoring Program, February 1982-December 1982. Report to Kansas Gas and Electric Company, Wichita.Ecological Analysts, Inc. 1984a. Gerald Gentleman Station Entrainment Study, 1983 Annual Report. Report to Nebraska Public Power District, Columbus.Ecological Analysts, Inc. 1984b. Wolf Creek Generating Station Preoperational Phase Environmental Monitoring Program, March 1983 -December 1983. Report to Kansas Gas and Electric Company, Wichita.EA Engineering, Science, and Technology, Inc. 1985a. Gerald Gentleman Station Entrainment Study, 1984 Annual Report. Report to Nebraska Public Power District, Columbus.EA Engineering, Science, and Technology, Inc. 1985b. Wolf Creek Generating Station Preoperational Phase Environmental Monitoring Program, February 1984 -December 1984. Report to Kansas Gas and Electric Company, Wichita.EA Engineering, Science and Technology Inc. 1985c. Biological and Chemical Monitoring in Turtle Creek Reservoir near Merom Generating Station, 1984.Report to Hoosier Energy Rural Electric Cooperative, Bloomington, Indiana.EA Engineering, Science, and Technology, Inc. 1986a. Wolf Creek Generating Station Operational Phase Environmental Monitoring Program, March 1985 -December 1985. Report to Kansas Gas and Electric Company, Wichita.3-15 EA Engineering, Science and Technology, Inc. 1986b. Biological and Chemical Monitoring in Turtle Creek Reservoir near Merom Generating Station, 1985.Report to Hoosier Energy Rural Electric Cooperative, Bloomington, Indiana.EA Engineering, Science, and Technology, Inc. 1987. M.R. Young Station, Nelson Lake, North Dakota, 1986 Aquatic Ecology Study. Report to Minnkota Power Cooperative, Inc., Grand Forks, North Dakota.Fryer, G. 1957. The food of some freshwater cyclopoid copepods and its ecological significance.

J. Animal Ecol. 26(3):263-285.

Jonasson, P.M. 1969. Bottom fauna and eutrophication, in Eutrophication:

Causes, Consequences, Correctives, pp. 274-305. NatTional Academy of Sciences, Washington, D.C.Kutkuhn, J.H. 1957. Utilization of plankton by juvenile gizzard shad in a shallow prairie lake. Trans. Amer. Fish. Soc. 87:80-103.

Lorenzen, C.J. 1966. A method for the continuous measurement of in vivo chlorophyll concentration.

Deep-Sea Res. 13:223-227.

Lovegrove, T. 1962. The effect of various factors on dry weight values.Rappt. Proces-Verbaux Reunions, Conseil Perm. Intern. Exploration Mer.156:86-91.

McQueen, D.J. 1969. Reduction of zooplankton standing stocks by predaceous Cyclops bicuspidatus thomasi in Marion Lake, British Columbia.

J. Fish.Res. Board Can. 26(6):1,605-1,618.

Parkos, W.G., T.A. Olson, and T.O. Odlaug. 1969. Water quality studies on the Great Lakes based on carbon-14 measurements on primary productivity.

Bull.17, Water Resources Research Center, Univ. of Minn. Graduate School. 121 pp.Saunders, G.W., Jr. 1969. Some aspects of feeding in zooplankton, in Eutrophication:

Causes, Consequences, Correctives, pp. 556-573. National Academy of Sciences, Washington, D.C.Siefert, R.E. 1972. First food of larval yellow perch, white sucker, bluegill, emerald shiner, and rainbow smelt. Trans. Amer. Fish. Soc.101:219-225.

Strickland, J.D.H. and T.R. Parsons. 1972. A Practical Handbook of Sea Water Analysis.

2nd edition. Fish. Res. Board Can. Bul. 167. 311 pp.Waite, S.W. 1981. Effects of cooling lake perturbations upon the zooplankton dynamics of Lake Sangchris, in The Lake Sangchris Study: Case History of an Illinois Cooling Lake (R.W. Larimore and J.A. Tranquilli, eds.). Ill.Nat. Hist. Surv. Bull. 32(4):342-357.

WAPORA, Inc. 1982. Preoperational Biological, Chemical, and Physical Monitoring at Merom Lake, Sullivan County, Indiana, Final Report. Report to Hoosier Energy Rural Electric Cooperative, Inc., Bloomington, Indiana.3-16 WAPORA, Inc. 1983. Turtle Creek Reservoir Biological, Physical, and Chemical Monitoring, 1982 Final Report. Report to Hoosier Energy Rural Electric Cooperative, Inc., Bloomington, Indiana.WAPORA, Inc. 1984. Biological, Physical, and Chemical Monitoring of Turtle Creek Reservoir, 1983 Final Report. Report to Hoosier Energy Rural Electric Cooperative, Inc., Bloomington, Indiana.Wetzel, R.G. 1975. Limnology.

Saunders, Philadelphia.

743 pp.Willhite, G.P., F.B. Cross, W.J. O'Brien, and Y.S. Yu. A Study of the Physical and Biological Effects of Thermal Discharge on LaCygne Lake. Principal Investigators' Report to the Office of Water Research and Technology, Department of the Interior, July 1976, Washington, D.C. 356 pp. including appendices.

3-17 Spatial Algal Standing Crop Neosho River Temporal Algal Standing Crop Neosho River 150-r E MLO CMLoc 10 MLAC 4 I 6 Q 125-100-75-50-25-0 01, m 9ww 1973 1975 1977 1979 1981 1983 1985 1987 1973 1976 1979 1982 1985 1988 I~~Seasonal Algal Standing Neosho River Crop Annual Algal Standing Crop Neosho River M Pro-t.Ake (1973-791 CMPro-Cpa.

(1980-85)MOpeamton (198"-7)E I 6 w 41 100" go-70 60 50-30-2O-10 0 JJA-ma 0 FEB-MAR APR JUN AUG--SEP OCT DEC 1973 1975 1977 1979 1981 1983 1985 1987 Figure 3-1. Phytoplankton standing crop trends In the Neosho River near Wolf Creek Generating Station, 1973-1987.

7 Spatial Algal Productivity Neosho River Temporal Algal Productivity Neosho River V~Lac 1 MLoc 10= Lfc 4 E 8 I I 1000.900-800-700-600-500-400-300-200 100 19*-~ ~G-2'IL 1973 1975 1977 1979 1981 1983 1985 1987 73 I-L0 k-, 1976 1979 1982 1985 Annual Algal Productivity Neosho River g988 Seasonal Algal Productivity Neosho River 8 I I (1973-79)R9 e-p-Or.(1980-65)EM 8 Oper7in ( 1986-6-A7 E 8 I I 300" 250-200-150-100-50-0'lFEB-MA~ APR JUN AUG-SEP OCT DEC 1973 1975 1977 1979 1981 1983 1985 1987 Figure 3-2. Phytoplarn-ton productivity trends in the Neosho River near Wolf Creek Generating Station. 1973-1987.

Spatial Algal Standing Crop WCGS Cooling Lake Temporal Algal Standing Crop WCGS Cooling Lake 25" or E-Lao- 2 CMLoc 6 CML=ao I 15-10 5-n .C 1981 1982 1983 1984 1985 1986 1987 Seasonal Algal Standing Crop WCGS Cooling Lake F JOMJOMJOF JOMJ OMJ OMJN 1981 1982 1983 1984 1985 1986 1987 Annual Algal Standing Crop WCGS Cooling Lake I w 20-18-14-12-10 8-4-2-0 1981 182 1983 1984 1985 4186 1987 FEB-MAR APR JUN AUG OCT--NOV DEC Figure 3-3. Phytoplarkton standing crop trends in the cooling lake near Wolf Creek Generating Station. 1981-1987.

Spatial Algal Productivity WCGS Cooling Lake Temporal Algal Productivity WCGS Cooling Lake E a I I Lo= 2 C Lc 6 LMo= a t E 11 0 I-1981 1982 1983 1984 1985 1986 1987 Seasonal Algal Productivity WCGS Cooling Lake F JOMJOMJOF JOMJOMJOMJ N 1981 1982 1983 1984 1985 1986 1987 Annual Algal Productivity WCGS Cooling Lake I a I I 100" 90-so-70-60-50-40-30-20-10-0 Lake-fllI (1981)(1982-65)opem167 (1966-67)5 I 100-90-so-70-60-50-40-30-20-10-j V sU L--J 0 r FEB-MAR APR JUN AUG OCT--NOV DEC 1981 1982 1983 1984 1985 1986 1987 Figure 3-4. Phytoplankton prodictivity trends in the cooling lake near Wolf Creek Generating Station. 1981-1987.

Spatial Zooplankton Dry Weight WCGS Cooling Lake Temporal Zooplankton Dry Weight WCGS Cooling Lake E E M IlII Loc 2 Loc 6 Loc 8 E 40 600-550-500-450-400-350-300-250-200-150-100-50-0 0 1981 1982 1983 1984 1985 1986 1987 Seasonal Zooplankton Dry Weight WCGS Cooling Lake I I I l I I I I I I I I I I I IIII I I 1 1 I I I i II 11 1 1 I I I I I I I I I I I I I I F JOMJOMJOF JOM JOMJ OMJ N 1981 1982 1983 1984 1985 1986 1987 Annual Zooplankton Dry Weight WCGS Cooling Lake E 0 5 I3 300" 250-200-150-100" 50-I I v FEB-MAR APR JUN AUG OCT DEC 1981 1982 19'83 19'84 19'85 19'86 19 87 Figure 3-5.Zooplankton dry weight standing crop trends in the cooling lake near Wolf Creek Generating Station. 1981-1987.

-.--*,-**s*~ I*~ .F~¶.1-it 200-180-160-140-12 10 80 60 40 20 0 Spatial Zooplankton AFDW Biomass WCGS Cooling Lake M Lc 2 COLo 6 Loc 8 Temporal Zooplankton AFDW Biomass WCGS Cooling Lake E'6 400-350-300-250a 200-150-100-50-0 1981 1982 1983 1984 1985 1986 1987 LJ I F JOCMJO MJO F JOCMJCM JO MJ N 1981 1982 1983 1984 1985 1986 1987 Annual Zooplankton AFDW Biomass WCGS Cooling Lake Seasonal Zooplankton AFDW Biomass WCGS Cooling Lake 400" 350-300-250-15D 100-50 2001 M Lake-fill (1981)Pro-op (1982-85)Operation (1986-87)SL .i E~175-150-125-100-75-50-25-Lm ig 0 FEB-MAR APR JUN AUG OCT DEC I I I I I I I 1981 1982 1983 1984 1985 1986 19837 Figure 3-6.Zooplankton ash free dry weight trends In the cooling lake near Wolf Creek Generating Station, 1981-1987.

WCCL Plankton Comparison Chi a vs. Dry Weight WCCL Plankton Correlation Chi a vs. Dry Weight E E 25-20-15-10-0 0-* Ph~,to CH a-0 ZACP Dry Wt"200"150 E*100 S*50 o N'250 y = 22.5x -68.7 r = 0.68 E+1 0 0 0.-...0 0 LO I 1981 1982 1983 1984 1985 1986 1987 WCCL Plankton Comparison Chi a vs. AFDW 0 5 10 Arnnal Chlorophyll a (mg/rm)WCCL Plankton Correlation Chi a vs. AFDW 15 a 0 E S 15-10-0..-0 Phyto CH a--.- ZoM AFMW"' .e'150'1 00 a E*50 _N;r E'a E y = 11.5x -27.1 r= 0.69 0 0 0 0 0 0 1981 1982 1983 1984 1985 1986 1987 n 0 5 10 15 Arnual Chlorophyll a (mg/m2)Figure 3-7. Relationship between phytoplarkton and zooplankton standing crop In the cooling lake near Wolf Creek Generating Station.

TABLE 3-1 PHYTOPLANKTON STANDING CROP AND PRODUCTIVITY AT NEAR WOLF CREEK GENERATING STATION, BURLINGTON, LOCATIONS 1, 10, AND 4 IN THE NEOSHO RIVER KANSAS, 1973-1979 Chlorophyll

! Concentrations (mg/m3)1 10 4 Mean Carbon Fixation Rates (mg3 C/m /hr)Date 12APR73 12JUN73 11SEP73 12DEC73 Mean 27MAR74 11JUN74 10SEP74 10DEC74 Mean 16APR75 10JUN75 9SEP75 3DEC75 Mean 25FEB76 6APR76 15JUN76 10AUG76 5OCT76 14DEC76 Mean 22FEB77 5APR77 9JUN77 9AUG77 4OCT77 13DEC77 Mean 22FEB78 25APR78 27JUN78 29AUG78 10OCT78 12DEC78 Mean 20FEB79 10APR79 12JUN79 7AUG79 9OCT79 11DEC79 Mean 1 10 4 Mean 1.58 8.13 13.41 3.57 6.67 8.77 0.70 4.14 10.50 6.03 34.97 3.67 10.97 21.67 17.82 38.67 18.33 16.00 7.27 7.17 6.33 15.63 12.27 21.33 1.53 35.90 8.54 8.20 14.63 35.04 12.00 30.11 24.63 37.41 20.07 26.54 3.38 6.40 2.66 21.53 11.52 48.06 15.59 6.57 0.77 1.83 9.17 4.59 33.33 1.84 10.34 6.57 13.02 11.67 15.37 14.23 16.00 9.73 2.99 11.67 12.43 14.53 1.47 28.54 6.32 7.61 11.82 29.85 10.33 21.29 20.68 14.86 2.95 16.66 2.50 5.61 5.61 17.07 10.21 50.96 15.33 1.93 2.23 19.75 3.13 6.76 7.54 0.80 0.08 9.00 4.54 34.00 2.27 6.70 7.87 12.71 17.33 16.63 12.77 16.00 19.73 2.53 14.17 11.60 14.33 1.53 43.25 5.26 7.96 13.99 28.98 9.54 23.42 27.98 14.56 2.15 17.78 1.42 5.41 4.64 16.62 11.11 50.64 14.97 1.78 5.18 16.58 3.35 6.72 7.63 0.76 2.26 9.56 5.05 34.10 2.59 9.34 12.04 14.52 22.56 16.78 14.33 13.09 12.21 3.95 13.82 12.10 16.73 1.51 35.90 6.71 7.92 13.48 31.29 10.62 24.94 24.43 22.28 8.39 20.32 2.43 5.81 4.30 18.41 10.95 49.89 15.30 3.51 29.61 28.00 5.45 16.64 18.29 15.57 7.30 24.83 16.50 90.90 14.24 44.26 32.18 45.40 21.66 58.82 45.79 26.23 41.58 21.39 35.91 67.42 55.25 5.30 34.28 15.09 15.42 32.13 6.71 6.51 25.80 28.24 26.64 12.19 17.68 3.35 12.00 11.18 43.60 50.85 6.99 21.33 17.45 0.15 5.62 22.92 11.54 125.98 6.87 36.82 10.39 45.02 20.35 62.25 45.97 80.69 46.35 5.27 43.48 69.00 45.02 5.75 33.34 13.30 14.47 30.15 8.08 4.90 24.54 45.14 13.45 1.55 16.28 1.15 10.42 16.59 56.46 46.04 6.19 22.81 3.96 22.34 35.06 5.21 16.64 18.90 11.40 5.94 22.92 14.79 130.65 2.54 24.66 10.38 42.06 20.82 64.23 41.07 95.59 79.01 4.84 50.93 77.00 45.32 7.04 45.35 13.22 13.99 33.65 6.56 5.76 30.52 51.68 17.66 2.58 19.13 0.92 9.77 16.65 61.55 48.32 6.72 23.99 3.74 25.98 31.53 5.33 16.64 18.21 9.04 6.29 23.56 14.28 115.84 7.88 35.25 17.65 44.16 20.94 61.77 44.28 67.50 55.65 10.50 43.44 71.14 48.53 6.03 37.66 13.87 14.63 31.98 7.12 5.72 26.95 41.69 19.25 5.44 17.70 1.81 10.73 14.81 53.87 48.40 6.63 22.71 Note: 1. Location 1 was in John Redmond Reservoir prior to 1976.2. Dash (-) indicates location not sampled.3-25 TABLE 3-2 PHYTOPLANKTON STANDING CROP AND PRODUCTIVITY AT LOCATIONS 1, 10, AND 4 IN THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION BURLINGTON, KANSAS, 1980-1987 Chlorophyll 1 Concentrations (mg/m 3 1 10 4 Mean Carbon Fixation Rates (mg C/m3 /hr)1 10 4 Mean Date 15APR80 17JUN80 23OCT80 16DEC80 Mean 28APR81 23JUN81 20OCT81 15DEC81 Mean 1MAR83 26APR83 30AUG83 13DEC83 Mean 28FEB84 17APR84 21AUG84 18DEc84 Mean 6MAR85 16APR85 25JUN85 27AUG85 22OCT85 10DEC85 Mean 4MAR86 29APR86 25JUN86 19AUG86 28OCT86 16DEC86 Mean 2MAR87 27APR87 22JUN87 24AUG87 23NOV87 28DEC87 Mean 7.31 25.48 31.28 27.41 22.37 30.57 9.28 13.71 17.28 17.71 53.30 5.34 9.88 10.94 19.86 126.46 1.74 45.44 73.96 61.90 1.17 9.67 1.14 3.86 0.59 1.73 3.03 142.75 35.80 27.44 4.33 9.09 83.93 50.56 29.94 43.97 31.71 13.01 29.66 8.81 20.97 11.37 18.56 14.93 44.28 10.20 16.33 14.30 21.78 49.96 5.05 22.98 6.16 21.04 143.83 1.68 48.02 75.80 67.33 2.32 13.82 0.66 4.21 1.48 2.30 4.13 123.35 44.89 36.45 5.61 7.69 62.51 46.75 25.58 40.55 21.45 12.63 39.37 17.34 26.15 7.91 28.06 12.76 14.12 15.71 33.74 10.85 21.52 15.87 20.50 50.23 5.17 17.77 5.81 19.74 127.40 1.64 37.35 77.27 60.92 2.20 14.85 1.06 4.03 0.92 2.64 4.28 118.17 46.58 47.35 5.82 6.80 51.81 46.09 23.22 44.32 20.39 14.84 42.20 15.29 26.71 8.01 24.84 18.47 20.03 17.84 36.20 18.02 17.85 15.82 21.97 51.16 5.19 16.88 7.64 20.22 132.56 1.69 43.60 75.68 63.38 1.90 12.78 0.95 4.03 1.00 2.22 3.81 128.09 42.42 37.08 5.25 7.86 66.08 47.80 26.25 42.95 24.52 13.49 40.79 16.31 27.38 2.84 77.78 7.74 0.48 22.21 18.39 22.58 13.81 48.90 25.92 29.61 13.68 1.91 0.70 11.48 1.60 3.48 63.83 123.86 48.19 2.10 63.68 1.21 4.08 0.47 1.68 12.20 295.06 129.12 142.08 15.19 27.70 826.04 239.20 31.75 74.14 10.77 6.82 30.87 2.69 33.14 3.47 1.49 10.20 30.42 21.64 14.34 40.09 26.62 29.26 13.37 5.07 2.84 12.64 2.12 2.25 84.82 96.79 46.50 6.01 66.84 2.93 4.41 0.86 3.48 14.08 403.68 128.08 149.53 27.45 37.68 697.16 240.60 21.58 87.59 7.15 7.45 30.94 2.64 108.78 4.45 1.18 29.26 22.39 23.15 13.80 43.35 25.67 28.24 13.14 4.99 3.26 12.41 1.76 0.07 112.34 100.45 53.66 3.68 68.78 2.14 4.21 1.30 3.29 13.90 350.68 103.56 189.78 27.19 32.06 705.89 234.86 12.07 82.99 7.91 8.10 27.77 2.72 73.23 5.22 1.05 20.56 23.73 22.46 13.98 44.11 26.07 29.04 13.40 3.99 2.27 12.18 1.83 1.93 87.00 107.03 49.45 3.93 66.43 2.09 4.23 0.88 2.82 13.40 349.81 120.25 160.46 23.28 32.48 743.03 238.22 21.80 81.58 8.61 7.46 29.86 Note: Dash (-) indicates location not sampled.3-26 I, I I..TABLE 3-3 PHYTOPLANKTON STANDING CROP AND PRODUCTIVITY AT LOCATIONS 2, 6, AND 8 IN THE COOLING LAKE OF WOLF CREEK GENERATING STATION BURLINGTON, KANSAS, 1981-1987 Chlorophyll a Concentrations (mg/mr 3 2 6 8 Mean 3 Carbon Fixation Rates (mg C/m /hr)2 6 8 Mean Date 24FEB81 28APR81 23JUN81 25AUG81 20OCT81 15DEC81 Mean 3MAR82 27APR82 22JUN82 31AUG82 19OCT82 7DEC82 Mean 1MAR83 26APR83 26JUN83 30AUG83 18OCT83 13DEC83 Mean 28FEB84 17APR84 19JUN84 21AUG84 160CT84 18DEC84 Mean 6MAR85 16APR85 25JUN85 26AUG85 22OCT85 10DEC85 Mean 4MAR86 29APR86 25JUN86 19AUG86 28OCT86 16DEC86 Mean 2MAR87 27APR87 22JUN87 24AUG87 23NOV87 28DEC87 Mean 25.5 11.5 10.3 11.5 14.4 16.5 15.0 7.0 12.3 6.0 16.9 9.7 6.3 9.7 5.4 11.4 11.6 10.9 15.2 4.4 9.8 4.9 8.0 12.4 11.1 12.6 5.4 9.1 1.8 4.6 9.0 6.7 20.1 14.7 9.5 5.3 19.6 7.6 8.6 14.3 7.5 10.5 10.4 4.5 7.4 7.6 4.9 3.5 6.4 10.2 4.9 2.7 6.0 7.0 11.2 7.0 2.6 7.0 6.7 7.6 12.4 4.6 6.8 4.1 5.7 6.9 9.2 9.8 3.9 6.6 4.9 6.8 4.4 5.6 9.3 5.3 6.0 3.3 5.3 6.0 6.9 15.2 25.7 10.4 4.8 17.7 9.2 6.3 7.9 10.5 9.4 8.4 3.8 6.0 7.3 4.8 4.9 5.9 6.0 6.9 6.7 7.8 10.6 7.1 7.5 2.9 9.3 8.7 7.6 22.2 20.8 11.9 4.3 19.5 8.8 8.0 10.3 10.6 10.3 7.5 4.5 7.5 11.1 3.4 5.3 6.6 17.9 8.2 6.5 8.8 10.7 13.9 11.0 4.8 9.7 6.4 12.3 11.1 5.5 8.3 4.8 8.6 9.3 10.1 12.5 4.2 8.2 5.3 7.2 7.8 8.2 10.8 5.9 7.5 2.7 6.4 7.9 7.1 19.2 20.4 10.6 4.8 18.9 8.5 7.6 10.8 9.5 10.1 8.8 4.3 7.0 8.6 4.4 4.6 6.3 115.8 4.1 24.5 2.8 14.8 32.3 32.4 10.9 88.9 0.5 0.2 62.8 1.5 27.5 2.0 35.2 32.7 0.5 15.9 0.2 14.4 1.0 28.2 26.3 40.8 11.8 10.8 19.8 7.1 11.7 11.0 11.8 31.8 12.3 14.3 10.9 115.3 80.4 62.7 93.5 50.2 68.8 6.5 14.6 4.0 10.6 8.9 40.2 2.8 5.2 2.4 7.1 23.9 13.6 4.1 44.6 0.1 0.0 80.3 0.1 21.6 2.1 17.6 22.0 1.0 10.3 1.4 9.0 6.3 26.9 7.0 22.0 7.8 15.0 14.2 6.8 7.9 13.2 16.7 32.9 15.2 15.4 12.1 100.9 67.9 73.3 50.1 71.6 62.7 8.7 12.9 4.0 9.9 9.1 0.0 26.8 16.1 33.9 10.4 15.7 17.2 5.5 10.9 19.2 15.5 38.0 13.0 17.0 9.9 94.6 61.0 69.1 62.1 73.3 61.7 7.0 11.2 5.8 13.6 9.4 78.0 3.4 14.9 2.6 10.9 28.1 23.0 7.5 66.8 0.3 0.1 71.6 0.8 24.5 2.0 26.4 27.4 0.8 13.1 0.8 11.7 2.4 27.3 16.5 32.2 10.0 13.8 17.1 6.5 10.2 14.5 14.7 34.2 13.5 15.6 11.0 103.6 69.8 68.4 68.6 65.0 64.4 7.4 12.9 4.6 11.4 9.1 Note: Dash (-) indicates location not sampled.3-27 TABLE 3-4 PLANKTON STANDING CROPS FOR SELECTED THERMALLY INFLUENCED LAKES IN MIDWESTERN AND GREAT PLAINS STATES Phytop1ankton Chlorophyll a (mg/m3)Year Mean (Min -Max)1987* 6.3 (3.4-11.1)

Zooplankton Biomass 3 (mg/mr ,AFDW)Mean (Min -Max)Lake WCCL, KS 1986*1985*1984 1983 1982 1981 Mean 10.1 10.6 7.5 8.2 8.2 11.0 8.8 (4.3-20)(1.8-26)(4.9-13)(3.9-15)(2.6-17)(2.7-26)53 91 67 40 56 95 123 75 (23-187)(14-257)(22-297)(15-88)(13-130)(18-183)(26-338)Reference Present Study EA 1987a EA 1986a EA 1985b Ecological Analysts 1984b Ecological Analysts 1983b Ecological Analysts 1982b Nelson Lake, ND Turtle Creek Res., IN Sutherland Res., NE Clinton Lake, IL LaCygne Lake, KS Lake Sangchria, IL 1986"* 27.0 (12.0-57)213 (42-647) EA 1987b 1985"*1984"*1983'1982*1981 Mean 1984**1983**1982**1981*1980'1975 1974 1973 Mean 1983 1982 1981 1980 1979 1978 Mean 1974*1973*1972 Mean 22.8 41.2 9.5 14.4 13.2 20.2 29.3 29.0 26.3 35.9 24.2 26.0 13.4 15.3 24.9 22.5 12.7 12.0 15.5 7.3 8.1 13.0 23.3 12.6 22.7 19.5 (9.6-47)(23-99)(5-14)(7-26)(6-24)(7.0-83)(10.4-91)(10.3-67)(13-104)(6-79)(3.4-57)(5.0-22)(1.5-48)(2.7-116)(2.6-37)(2.0-38)EA 1986b EA 1985c WAPORA 1984 WAPORA 1983 WAPORA 1982 EA 1985a Ecological Analysts Ecological Analysts Ecological Analysts Ecological Analysts Ecological Analysts Ecological Analysts Ecologcial Analysts Ecological Analysts 1984a 1983a 1982a 1981a 1981b 1981b 1981b 1981b Willmore Willmore willmore willmore Willmore Willmore 1985 1985 1985 1982 1982 1982 (1.5-259)(0.9-31)(1.4-43)Willhite et al 1976 Willhite at al 1976 Willhite at al 1976 1975-76* -78 (43-164) Waite 1981 Lake Shelbyville, IL 1975-76 -82 (18-134) (preserved samples)Note: Asterisk (*) indicate number of generating units operating during the year.3-28 TABLE 3-5 ZOOPLANKTON BIOMASS STANDING CROP AT LOCATIONS 2, 6, AND 8 IN THE COOLING LAKE OF WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1981-1987 Dry Weight (mg/m3 ) Ash-Free Dry Weight (mg/u3)2 6 8 Mean 2 6 8 Mean Date 23FEB81 28APR81 23JUN81 25AUG81 19OCT81 16DEC8I Mean 3MAR82 27APR82 22JUNB2 31AUG82 190CT82 7DEC82 Mean 1MAR83 26APR83 26JUN83 29AUG83 18OCT83 13DEC83 Mean 28FEB84 17APR84 19JUN84 21AUG84 19OCT84 18DEC84 Mean 5MAR85 15APR85 24JUN85 26AUG85 22OCT85 10DEC85 Moan 3MAR86 29APR86 25JUN86 18AUG86 28OCT86 15DEC86 Mean 3MAR87 28APR87 22JUN87 24AUG87 Mean 228 92 205 86 171 156 190 194 44 24 117 76 108 30 75 17 53 153 99 71 59 87 27 64 82 87 68 252 1052 231 46 392 88 344 78 219 71 37 265 226 149 301 93 200 83 169 338 143 100 153 29 128 148 109 161 61 66 115 190 117 57 65 83 27 90 107 72 34 43 34 53 95 75 56 103 136 97 87 196 72 115 106 154 61 60 201 315 150 43 93 95 76 77 61 52 67 93 78 89 73 199 261 676 94 187 35 242 119 128 169 61 142 360 163 79 265 65 79 122 338 186 96 179 58 150 152 150 178 52 45 116 133 112 44 70 50 40 122 103 71 51 61 43 70 85 84 66 185 483 335 76 258 65 234 101 167 100 53 203 300 154 141 150 120 79 123 195 56 169 55 113 118 175 178 23 18 71 32 83 17 53 14 16 130 84 52 44 60 20 32 63 31 42 63 297 29 22 54 66 88 46 162 22 14 136 82 77 61 66 23 23 43 338 127 62 108 26 99 127 105 148 56 60 90 183 107 29 45 75 18 84 101 59 30 36 29 15 88 24 37 30 59 40 70 65 41 51 91 127 48 37 64 257 104 24 71 28 32 39 101 30 41 57 21 70 31 42 47 112 76 42 67 25 62 81 88 137 38 82 133 93 40 187 25 57 77 338 161 59 138 40 106 123 140 163 40 39 80 108 95 23 49 44 17 92 92 56 35 46 35 23 74 29 40 47 156 48 45 62 44 67 73 126 69 30 94 157 91 42 108 25 37 53 Note: Dash (-) indicates location not sampled.3-29

4. MACROINVERTEBRATES

4.1 INTRODUCTION

The macroinvertebrate fauna of freshwater lakes and rivers is mostly composed of aquatic insects, worms and roundworms.

Their distribution within an aquatic macrohabitat is determined by the habits and habitat requirements of the various macroinvertebrate taxa. Habitat variables include current velocity, substrate type, organic matter content, temperature, and water depth. Habits can be mode of feeding (filterers, shredders, grazers, or predators) or the drifting phenomenon which occurs in some taxa found in lotic habitats.Voltinism of aquatic insects can affect density rates and make them highly variable on an annual basis.Macroinvertebrates can either be primary consumers (detritivores-herbivores) or secondary consumers (carnivores) in aquatic food chains. Primary consumers are usually grazers, shredders, or filterers that feed on phytoplankton, bacteria, aquatic plants, protozoa, and organic detritus.

Secondary consumers are predaceous feeding on zooplankton and other macroinvertebrates.

Most fish are secondary or tertiary consumers that utilize macroinvertebrates in their diet either as fingerlings or adults. The diversity of macroinvertebrates gives a relative index of species composition, and is also an excellent indicator of water quality due to the narrow tolerance range of some macroinvertebrates to pollutants.

Macroinvertebrate studies at WCGS began on the Neosho River in 1973 and on the Wolf Creek Cooling Lake (WCCL) in 1981. These studies were directed towards 4-1 the determination of taxa composition, macroinvertebrate density, and diversity from a qualitative and quantitative sampling regime. The primary purpose of this study was to examine spatial and temporal trends in the macroinvertebrate communities for effects of WCGS operation, which began in August 1985.A macroinvertebrate occurring near WCGS and warranting special attention is the 1.1' asiatic clam, Corbicula fluminae.

The first report of Corbicula near WCGS was in August 1986 when immature clams were collected at a long term monitoring site located on the Neosho River (EA 1987). Quarterly and bimonthly surveys at these sites since 1974 had not produced evidence of Corbicula until 1986. The occurrence of Corbicula in the vicinity of intake structures poses potential biofouling problems associated with these clams as indicated by IE Bulletin 81-03 issued by the U.S. Nuclear Regulatory Commission (April 10, 1981), regarding flow blockage of cooling water to safety system components.

Thus, sampling efforts associated with WCGS environmental monitoring program were increased in the fall of 1986 following the August report of Corbicula and the initiation of planned annual surveys began in 1987 to monitor Corbicula in the vicinity of WCGS.4.2 METHODS Macroinvertebrate samples were collected from the Neosho River either quarterly or bimonthly from 1973 to 1987 except in 1982, 1983 and 1984 when sampling was not conducted.

In all years sampling occurred at Location 10 upstream of the confluence with Wolf Creek and at Location 4 downstream of Wolf Creek.Location 1 in the John Redmond Reservoir tailwaters was added to the sampling regime in 1976 (Figure 1-1). Macroinvertebrate samples were collected from the 4-2 WCCL bimonthly from 1981 through August 1987. Sampling at Location 2 near the WCGS discharge and Location 6 near the dam began in 1981. Location 8 near WCGS intake was added to the program in 1984.Quantitative duplicate benthic macroinvertebrate samples were collected from Locations 4 and 10 on the Neosho River and Locations 2, 6, and 8 on the WCCL with a ponar dredge (area sampled=530 cm 2). The texture of sediment from each ponar sample was visually characterized and then sieved on a U.S. Standard No.30 mesh (0.595 mm) screen. The sieve residue was preserved with 10 percent formalin and stained with rose bengal (Mason and Yevich 1967) in appropriately labeled containers.

Qualitative river samples were obtained by seining and hand picking rocks at Locations 1, 10, and 4 on the same schedule as the ponar collections.

Macroinvertebrates encountered were placed in vials and preserved with 10 percent formalin.In the laboratory, ponar samples from the Neosho River and WCCL were further washed on a U.S. Standard No. 30 mesh screen prior to manually separating macroinvertebrates from debris under 1OX magnification.

Oligochaeta and Chironomidae were mounted in a nonresinous mounting medium (CMC-10) on glass slides and identified under a compound microscope at 78.75-1250X magnification.

Other macroinvertebrates were identified under a binocular dissecting microscope at 10-70X magnification and preserved in 70 percent ethanol. All organisms were identified to the lowest positive taxonomic level using appropriate references.

Organisms from the qualitative samples were identified and counted.4-3 Overall trends for densities, taxa composition, and diversity were examined by graphing average annual values for each location.

For this report, densities from the benthic macroinvertebrate ponar samples were reported as the mean number of organisms per meter square (No./m 2) annually, and by collection dates. Contribution of major taxonomic groups to the benthos community was graphed as average percent of the annual total density. Identified taxa numbers were reported annually for each sampling location.

Diversity indices were calculated using Shannon's (1948) equation using base-2 logarithms and graphed by location for each sampling years. The resulting figures and tables permitted detailed examination of annual temporal cycles and variances between locations.

Sampling efforts for Corbicula during the 1986 survey occurred in August, November and December at Locations 10 and 4, in the vicinity of the makeup water pumphouse (MUSH), and at Hartford Rapids, all in the Neosho River.Sampling in the WCCL was conducted at the three established monitoring sites (Locations 2, 6, and 8), at the Makeup Water Discharge Structure (MUDS), and at the WCGS circulating water intake.The 1987 survey for Corbicula near WCGS was conducted on 30 September

-1 October. Samples from the Neosho River were taken near the low water dam at Burlington, at U.S. 75 Highway bridge, and below John Redmond Dam in the vicinity of the MUSH. The Neosho River was also sampled at sites above John Redmond Reservoir between the 310 county road bridge and the public launch site at Hartford.

Wolf Creek was sampled approximately 50 meters upstream of the FAS-10 bridge. The WCCL was sampled in the vicinity of the MUDS, circulating water screenhouse (CWSH), and the service spillway (Figure 1). Additional 4-4 samples were taken along the "hilltop gravel" beach west of the service spillway.Sampling was conducted with three gear types. Ponar grabs were taken at most sites where substrates were suitable.

A clam rake with a 1/8-in mesh liner was used at some sites and a 15-ft common-sense seine of 1/8-in mesh was used in the Neosho River where currents allowed kick seining. Water temperature and depth was recorded at each site and substrates were visually characterized and recorded.4.3 RESULTS AND DISCUSSION 4.3.1 Neosho River Macroinvertebrates Macroinvertebrates collected during the 1973-1987 Neosho River study were represented by 179 taxa (Table 4-1). The benthic population was dominated by four taxonomic groups that accounted for 65 percent of the total identified taxa; aquatic midges composed 30 percent (54 taxa; Diptera), mayflies 15 percent (26 taxa; Ephemeroptera), aquatic worms 11 percent (20 taxa;Oligochaeta), and caddisflies 9 percent (16 taxa; Trichoptera).

Other groups represented in the collections included mollusks (11 taxa), aquatic beetles (10 taxa; Coleoptera), leeches (9 taxa; Hirudinea), dragonflies (9 taxa; Odonata), miscellaneous arthropoda (6 taxa), stoneflies (4 taxa; Plecoptera), true bugs (4 taxa; Hemiptera), hydras (2 taxa; Cnidaria), planarians (2 taxa;Platyhelminthes), bryozoans (2 taxa; Entoprocta), and Collembola and Arachnida (one taxa each).4-5 Macroinvertebrate taxa collected annually from the Neosho River ranged from a low of 26 taxa to a high of 132 taxa in 1973 and 1976, respectively (Table 4-2). The 1973 collection was restricted to Locations 10 and 4, whereas Locations 1, 10 and 4 were sampled in 1976. Annual trends of macroinvertebrate taxa showed no spatial differences between locations.

The highest fluctuation in taxa numbers occurred in 1980 with 60 identified taxa from Location 10 and 46 from Location 4 representing a 23 percent difference.

In 1977 an equal number of taxa was collected from both locations (Table 4-2).On a temporal basis, taxa numbers showed an increase from 1973 (26 taxa) to peak taxa numbers in 1976 (132 taxa) when Location 1 was added. Stable taxa numbers occurred from 1976 to 1979 with a decline present in the 1979 and 1981 sampling years. Total taxa collected in 1985 showed a 32 percent decline from 1981 levels, and then gradually approached pre-1985 levels in 1986 and 1987 (Figure 4-1). The declines in taxa number during the 1985 sampling season were attributed to high river flows, which affected sampling efficiency.

Macroinvertebrate densities in the Neosho River ranged from a mean annual low of 48/mi 2 in 1985 to a high of 9,156/mi 2 in 1976 (Table 4-3). Total annual densities showed a gradual increase in numbers from 1973 to 1975, increased substantially in 1976 and 1977, and then stabilized between 2,000 and 5,000/mi 2 from 1975 to 1981 when the study was suspended.

In 1985 when the study was resumed, the lowest annual density of the entire study occurred, apparently as a result of the increased water flows which occurred during that year. Due to the high river flows, collection schedules were altered and the sampling efficiency was affected contributing to the low densities.

Density recovered eight fold in 1986 and remained stable in 1987. The same trend in total 4-6 density occurred for both locations during the study.Annual density lows were 65 and 48 specimens per square meter at Locations 10 and 4, respectively, with both occurring during the 1985 collections (Table 4-3). Respective maximum annual densities were 12,329 and 5,984/m2 , with both occurring during the 1976 collection season. Densities were highly variable within collection years, and these differences were attributed to climatic and limnological (e.g., flow) changes within the system and to life cycle patterns of the macroinvertebrates.

Of the four major macroinvertebrate taxa groups collected from the Neosho River during the study period, only oligochaetes and caddisflies showed location preferences.

Oligochaete occurrence was annually more dominant at Location 4 than at Location 10, except in 1975 and 1977 (Figure 4-2). This difference would be attributable to variations in substrate types between the two locations.

Location 10 is a riffle area with a rocky substrate while Location 4 is primarily a sand silt substrate.

Annual caddisfly composition showed a preference for Location 10 except in 1977 when caddisflies were more numerous at Location 4 (Figure 4-2). Caddisflies often show preferences for hard substrates (e.g., rocks such as those predominant at Location 10) due to their habit and habitat requirements.

Mayflies showed a mixed distribution between Locations 10 and 4 with no obvious location preference (Table 4-4). Temporally the mayfly composition in the quantitative samples showed an initial high rate, then declined and fluctuated for the remainder of the study. Midges on an annual basis showed no preference for Location 10 or 4, which would be characteristic of the ubiquity of this 4-7 group. Overall annual trends of midges showed a steady increase in their contribution to the macroinvertebrate composition from 1973-77. Their contribution remained fairly stable from 1977-1985 but declined in the 1980 season. The lowest occurrence of midges occurred in 1986 when they made up less than 6 percent of total density.Diversity or species richness of macroinvertebrates in the Neosho River was considered moderate from 1975 to 1981 with no consistent spatial difference (Figure 4-1). Diversity during the period ranged between a high of 3.52 in 1980 and a low of 2.37 in 1981 (Table 4-3). Although Location 4 often had slightly higher diversity, Location 10 had the highest annual diversity at 3.81 in 1980. The high diversity peaks in 1980 and 1976 at Location 10 were due to the increased densities of stoneflies (Plecoptera);

in 1980 stonefly density 2 2 was 507/m , and in 1976 it was 464/m .The next highest density of stoneflies 22 occurred in 1979 with 153/m , and stonefly densities did not exceed 50/m 2 in other years (Table 4-4).Diversity trends during the sampling years of 1985 to 1987 show the lowest annual diversity of 0.98 recorded in 1985 (Table 4-3). An increase in diversity was seen in 1986, and stabilization occurred in 1987 at approximately 1986 levels. The low diversity from the 1985 samples was attributed to the high river flows which occurred during most of the year and affected sampling efficiency.

A recovery period was evident during the sampling years of 1986 and 1987 when diversity increased to pre-1985 levels.Linear regression was performed using annual densities and annual flows to examine the relationship between these two parameters.

This analysis gave a 4-8 correlation of r=0.75 (Figure 4-1), indicating a relatively strong, inverse relationship between the two variables.

High flows can either affect sampling efficiency or the macroinvertebrate community itself by loss of habitat, particularly current velocity parameters, or actual removal of organisms by the increased stream load and its degrading effects. Increased flows can also affect the macroinvertebrate composition and the diversity of the community.

Macroinvertebrate studies of the Neosho River at the John Redmond Reservoir (JRR) tailwaters and upstream and downstream of the confluence with Wolf Creek have been conducted since 1973. Aquatic oligochaetes, mayflies, stoneflies, net-spinning caddisflies, and midge flies have been dominant organisms.

No long-term patterns or empirical differences have been found that were attributable to the construction and/or operation of the WCCL and WCGS. The data have been highly variable which was attributed to fluctuating river flows that undoubtedly affected organism abundances but also greatly influenced sampling efficiency.

The potential for WCGS to impact the Neosho River macroinvertebrate community has been minimal based on low diversion rates from the JRR tailwaters and the lack of substantial discharge from the WCCL.4.3.2 Cooling Lake Macroinvertebrates Benthic macroinvertebrates collected from the Wolf Creek Cooling Lake (WCCL)were represented by 70 taxa from the study period of 1981-1987 (Table 4-1).The benthic community was primarily dominated by the aquatic midges (Diptera)which accounted for 41 percent (29 taxa) of the taxa identified.

Two other taxonomic groups which are important to lentic environments and collected from the WCCL were the oligochetes Naididae (7 taxa) and Tubificidae (12 taxa), 4-9 comprising 10 percent and 17 percent of the taxa total respectively.

Other groups represented in the collections were the Mollusca (7 percent, 5 taxa), Ephemeroptera (7 percent, 5 taxa; mayflies), Trichoptera (6 percent, 4 taxa;caddisflies), and Odonata (4 percent, 3 taxa; dragonflies).

Groups represented by a single taxon included the Cnidaria, Platyhelminthes, Nematoda, Arthropoda and Arachnida.

Annually, the number of taxa identified from the WCCL remained fairly stable during the 7 year study ranging from a high of 32 taxa in 1981 to a low of 19 in 1987 (Table 4-2). Spatially, no consistent pattern was evident between sampling locations relative to the number of taxa collected during the study period (Figure 4-3). However, data collected show differences in the macroinvertebrate composition at certain locations due to differential location or substrate preferences between taxa groups (Figure 4-3).For example, Locations 2 and 8 were dominated by the midge taxa group (Figure 4-3 and Table 4-5), based on average percent composition of the annual total macroinvertebrate density. The one exception to the pattern occurred in 1981 during lake filling, when Location 2 was dominated by tubificids and Location 8 was not sampled. The midge dominance at Locations 2 and 8 may be due to the shallower depth of these sampling locations, (4.5 and 7.5 meters respectively) when compared to Location 6 (19 meters). Another factor which could affect macroinvertebrate distribution at the three sampling locations was substrate composition, because prior to lake filling Location 2 was a cultivated field, Location 6 was an old quarry site, and Location 8 was an area of alluvial deposits.

Spatially, tubificid taxa were predominantly more abundant at Location 6 during the study period, except in 1981 when tubificids were more 4-10 abundant at Location 2 (Figure 4-3 and Table 4-5).Naidid worms never dominated any sampling locations in terms of benthic composition during the study. The highest contribution by the naidids occurred in 1981-82 at Locations 2 and 6, and in 1986-87 at Location 6 only (Figure 4-3 and Table 4-4). During 1983-85, naidids were almost nonexistent in the benthic samples; the same was true for Location 8 throughout the study period.Macroinvertebrate densities in WCCL were typical of new reservoirs, which often exhibit initially high annual densities and then show a decline in numbers to a stable density (Figure 4-4). In WCCL, initially high macroinvertebrate densities occurred during the lake filling phase (1981-1982) and reflected the incorporation of two new temporary nutrient sources, one by the use of a shallow eutrophic reservoir (JRR) as the primary source of water for lake filling, and secondly by the inundation of vegetation.

A decline in macroinvertebrate densities occurred in 1983 at the start of the preoperational phase (1983-85), and densities then remained stable for the duration of the study, reflecting the process of the lake assuming its own character.

Macroinvertebrate densities during the three WCCL operational phases ranged from a high of 1,521/m to a low 170/m in the lake fill phase (1981-82) and operational phase (1985-87), respectively (Table 4-6). Preoperational and operational densities (1983-1987) averaged 286/m2 for the five years, whereas 2 the two year lake-filling average was 1,311/m .Spatially, densities were variable between the sampling locations during preoperational and operational sampling years (1983-1987) with no location showing consistently higher densities.

However, during lake-fill macroinvertebrate densities were 50 percent greater at Location 2 when compared to Location 6. The density 4-11 1j_V! differences between the two locations is probably due to the previously mentioned variations in depth and substrate type.1;.GDifferent seasonal trends in benthic densities were evident for the three phases of the study (Figure 4-4). During lake-fill (1981-82), densities peaked in February-March and June, with the February-March peak being the greatest.Remaining sample periods during the lake-fill phase were reasonably stable with the lowest densities occurring in August. In the preoperational phase, peak densities occurred in April while the remaining collection periods were variable.

During the operational phase, peak densities occurred in the February-March samples, declines were evident in April, and densities then remained fairly constant for the later collection dates.Seasonal benthic densities showed the relatively high density which occurred during the lake-fill phase, the decline during the preoperational phase, and the stability during the operational phase that was also evident in the annual benthic density (Figure 4-4). This decreasing density pattern was evident for nearly all sampling seasons. Exceptions included the February-March season, when a minor density increase occurred between the preoperational and operational phases, and the April season when a minor decrease occurred between the lake fill and preoperational phases. Overall annual and seasonal density trends were considered representative of a new lake that was initially filled with eutrophic water and experienced nutrient loading, and thereafter gradually assumed its own character.

Diversity or species richness of the WCCL macroinvertebrate community showed no annual trends or consistent spatial differences during the study period. Low 4-12 benthic diversity occurred in 1985 for Locations 2 and 6 at 1.14 and 0.88 respectively.

Diversity maxima occurred at Location 6 in 1981 (1.89) and Location 2 in 1982 (2.00), and even these values were considered in the low to moderate range. On an average annual basis maximum diversity occurred in 1981 at 1.76, and the low was 1.06 in 1985 (Table 4-6). There were no evident changes in benthic diversity attributable to WCGS. Incidents of variations in benthic diversity both annually and seasonally could be attributed to climatic and limnological changes within the system.Obvious differences were noted in diversity between the Neosho River (annual high 3.52) and WCCL (annual high 1.76). These differences reflect variations in the habitat requirements of certain benthic groups and the greater variety of habitats in the river. Two groups poorly represented in the WCCL but important in the Neosho River were the Ephemeroptera (mayflies, 26 taxa from the Neosho River) and Trichoptera (caddisflies, 16 taxa from the Neosho River).Both of these groups contain primarily lotic inhabitants.

However, four taxa from each group were identified from the Wolf Creek Cooling Lake.The quantitative dominance of the burrowing benthic Tubificidae and Chironomidae in the WCCL reflected the characteristic soft ooze-like substrates rich in organic matter typical of the lake bottom (Reid 1961). This benthic fauna is typical of most depositional lake bottom sediments (Brinkhurst 1974)and is similar to that of other midwestern reservoirs:

John Redmond Reservoir, Kansas (Funk and Ransom 1977); Keystone Reservoir, Oklahoma (Ransom and Dorris 1972); Lakes Matanzas, Quiver and Chautauqua, Illinois (Paloumpus and Starrett 1960).4-13 Benthic macroinvertebrates in the WCCL have been sampled bimonthly since 1981 when the cooling lake was initially filled. The 1986 program represented the second annual study since WCGS began operation and data were similar to previous lake-filling and preoperational data. The benthic fauna of the WCCL is fairly typical of lakes in general and midwestern reservoirs in particular.

Quantitative dissimilarities in the faunas from the three sampling sites reflected differences in their respective depths, substrate composition, and organic matter content. The data have exhibited high annual variation from 1981 through 1987 that likely reflects various ecological, climatic, and limnological factors. Operation of WCGS has caused no apparent changes in the macroinvertebrate community.

Declines in macroinvertebrate abundance that occurred after WCCL was initially filled represent normal responses to changes in productivity that occur as reservoirs begin to age.4.3.3 Corbicula Distribution and Abundance Asiatic clam (Corbicula fluminea) densities in the Neosho River below Burlington ranged from no clams to 9.4/mi 2 at Location 10 and from 47.3 to 57.6/m2 at Location 4 in 1986. The 1987 samples showed a 41 percent increase (57.6 to 79.9 m 2) at Location 4 and essentially no change at Location 10, (Table 4-7). These densities are considered indicative of pioneer populations.

In the New River, Virginia, Corbicula increased from 30/m2 to more than 10,000/m2 in a two year period (Cherry et al. 1986).Additional efforts to monitor the distribution of Corbicula in the vicinity of WCGS in 1987 (30 September

-1 October) showed an expansion upstream to the U.S. 75 bridge where one specimen was collected (Table 4-8). Additional 4-14 locations sampled included FAS-1O Bridge on Wolf Creek, Burlington low water dam, John Redmond Reservoir (JRR) tailwaters, and the Hartford Boat Ramp area above JRR. Three Corbicula were collected near the Burlington Dam which is downstream of the U.S. 75 bridge, but no Corbicula were collected at the remaining sampling Locations (Table 4-8).Corbicula surveys in the Neosho River near WCGS have shown a gradual increase in densities at downstream locations and an increase in distribution upstream to the U.S. 75 bridge. Further expansion of Corbicula distribution appears to be limited by substrate types found in the Neosho River upstream of Burlington.

Corbicula are reported from nearly all substrate types, however, optimum conditions seem associated with loose gravel in shallow pools below riffles and sandy or rock-bottom streams of intermediate flow (Neck 1986).Substrate types below JRR consists of extensive natural bedrock and coupled with high current velocities would be a limiting factor in this area. The reservoir itself has rich ooze and mud substrates which would also limit Corbicula colonization.

The potential for Corbicula to become established in JRR or its tailwaters near the MUSH, which could be a possible vector for distribution into WCCL, appears to be limited due to the inhospitable substrate types.Asiatic clams have not been collected from the Wolf Creek Cooling Lake.Sampling in the WCCL after the reported occurrence in the Neosho River consisted of 70 ponar grabs since August of 1986. Forty-two of those samples were taken bimonthly from established monitoring sites near the WCGS Intake (Location 8), the main dam of WCCL (Location 6), and an uplake site near the 4-15 WCGS discharge (Location 2). The additional 28 samples were taken in the 1986 and 1987 Corbicula surveys near the CWSH and MUDS.The apparent lack of Corbicula upstream in JRR minimizes the potential that VCCL will have a future population because makeup water for the cooling lake is pumped from the Neosho River immediately below the JRR stilling basin. It is generally accepted that other than man-mediated dispersion, downstream drift of the planktonic larval stage is the main factor affecting range extensions.

Therefore, before Corbicula could be introduced to the WCCL via makeup water, it would have to occur upstream in JRR.4.4

SUMMARY

AND CONCLUSIONS 4.4.1 Neosho River Macroinvertebrate Studies Macroinvertebrates studies of the Neosho River at the John Redmond Reservoir (JRR) tailwaters as well as upstream and downstream of the confluence with Wolf Creek have been conducted since 1973. Aquatic oligochaetes, mayflies, stoneflies, net-spinning caddisflies, and midge flies have been dominant organisms.

No long-term patterns, empirical, or statistical differences have been found that suggested any alterations attributable to the construction and/or operation of the WCCL and WCGS. The data have been highly variable which has been attributed to fluctuating river flows that undoubtedly affects organism abundances but also greatly influences sampling efficiency.

The macroinvertebrate monitoring program on the Neosho River was reimplemented in 1985 to coincide with start up of WCGS after the program was discontinued in 4-16 1982. High, variable flows in 1985 resulted in low sample recovery and benthic densities that approached the lowest recorded since monitoring was initiated in 1973. Species richness and abundance improved substantially in 1986 as flows were comparatively stable and low. In 1987, the number of taxa encountered remained stable, and mean annual ponar density exhibited continued improvement.

The potential for WCGS to impact the Neosho River macroinvertebrate community has been minimal based on low diversion rates from the JRR tailwaters and the lack of substantial discharge from the WCCL.4.4.2 WCCL Macroinvertebrate Studies Benthic macroinvertebrates in the WCCL have been sampled bimonthly since 1981 when the cooling lake was initially filled. The benthic fauna of the WCCL is fairly typical of lakes in general and midwestern reservoirs in particular.

The data have exhibited high annual variation from 1981 through 1987 that likely reflects various ecological, climatic, and limnological factors. While quantitative dissimilarities in the faunas from the three sampling sites reflected differences in respective depths, substrate composition, and organic matter content. Operation of WCGS caused no apparent changes in the macroinvertebrate community during the initial two years of operation.

Although mean annual benthic macroinvertebrate densities in 1987 (170 organisms/m2 ) were at a low for the seven-year study, densities declined annually through 1984 after peaking in 1982 (1,521/m2

). Mean annual densities increased slightly in 1985 (332/m 2), the first year of station operation, but have since continued to decline. Down lake densities at the deep water (17-22 m) location near the main dam were primarily responsible for the annual trend.4-17 At the organism level, primarily oligochaetes and chironomids influenced the trend as both groups declined annually after peaking in 1982 except for tubificid which recovered in 1985 and than declined to relatively low densities in 1986 and 1987. The 1985 recovery was due almost exclusively to mean annual tubificid densities at Location 6, which were the second highest recorded for the WCCL study. Apparent changes in the WCCL benthos reflect normal responses of pioneer organisms to newly-filled reservoirs and could be expected independent of WCGS operation.

4.4.3 Asiatic Clam-Corbicula The 1987 survey for Asiatic Clams in the vicinity of Wolf Creek Generating Station verified that Corbicula fluminea remained established in the Neosho River below Burlington, Kansas. Further distribution of Corbicula upstream of Burlington appears to be limited by inhabitable substrate types in these reaches of the Neosho River. Corbicula has not been found in the WCCL.Possible colonization of WCCL could occur by man-mediated dispersion or by uptake of Corbicula larvae via the MUSH into WCCL. The absence of an established population in the JRR tailwaters or further upstream makes the second mode of introduction unlikely.

However, substantial expansion in abundance and upstream dispersion would increase the likelihood that Corbicula could eventually occur in the WCCL.Several factors which could possibly affect Corbicula distribution and abundance include substrate types in both the river and the cooling lake, anoxic conditions in the stratified cooling lake (Sickel 1986), and low winter temperatures and low flows in the Neosho River.4-18

4.5 REFERENCES

Brinkhurst, R.O. 1974. The Benthos of Lakes. St. Martin's Press, New York.190 pp.Cherry, D.S. et al. 1986. Corbicula fouling and control measures at Celco Plant, Virginia.

Pages 69-82 in Special Edition No. 2, Amer.Malacological Bulletin.EA Engineering, Science, and Technology, Inc. 1987. The Occurrence and Abundance of Asiatic Clams (Corbicula fluminea) in the Vicinity of Wolf Creek Generating Station. Report to Kansas Gas and Electric, Burlington, Kansas.Funk, F.L. and J.D. Ransom. 1977. Benthic diversity and related physico-chemical features of John Redmond Reservoir, 1971-72. Emporia State Res.Stud. 26:23-26.Mason, W.T. and P.P. Yevich. 1967. The use of phloxine-B and rose bengal stains to facilitate sorting benthic samples. Trans. Am. Microsc. Soc.86(2):221-223.

Neck, R.W. 1986. Corbicula in public recreation waters of Texas: habitat spectrum and clam-human interactions.

Pages 179-184 in Special Edition No. 2, Amer. Malacological Bulletin.Paloumpus, A.A. and W.C. Starrett.

1960. An ecological study of benthic organisms in three Illinois river flood plain lakes. Am. Midl. Nat.64:406-435.

Ransom, J.D. and T.C. Dorris. 1972. Analysis of benthic community structure in a reservoir by use of diversity indices. Am. Midl. Nat. 87(2):434-447.

Reid, G.K. 1961. Ecology of Inland Waters and Estuaries.

Van Nostrand Reinhold Co., New York. 375 pp.Shannon, C.E. 1948. A mathematical theory of communication.

Bell System Tech. J. 27:379-423, 623-656.Sickel, J.B. 1986. Corbicula population mortalities:

factors influencing population control. Pages 89-94 in Special Edition No. 2, Amer.Malacological Bulletin.Wetzel, R.G. 1975. Limnology.

Saunders, Philadelphia.

743 pp.4-19

!'771 -.1. 1177': Annual Macroinvertebrate Taxa Neosho River Spatial Benthic Densities Neosho River I I I I 0 1973 1975 1977 1979 1981 1983 1985 1987 0 Spatial Benthic Diversity Neosho River 1973 1975 1977 1979 1981 1983 1985 1987 Annual Density vs. Annual Flow Neosho River S 9 E~10000-1000'100-0 0 0 y=-1.90x + 9.27 r=0.75 0 101 501 0.00 1973 1975 1977 1979 1981 1983 1985 1987 0 1000 10000 Arnnual Mean Flow (cfs)Figure 4-1.Macroinvertebrate density end diversity in the Neosho River near Wolf Creek Generating Station, 1973-1987.

Oligochaete Contribution to Benthos Neosho River Mayfly Contribution to Benthos Neosho River 0 0 75-50" 25-Loc 1(Loc 4 I 3I 0 F-j0 11091 14JmI j Not Sampled 1973 1975 1977 1979 1981 1983 1985 1987 Js Midge Contribution to Benthos Neosho River 1973 1975 1977 1979 1981 1983 1985 1987 Caddisfly Contribution to Benthos Neosho River 7T-4.0 0 I a I a a Not 0 1973 1975 1977 1979 1981 1983 1985 1987 1973 1975 1977 1979 1981 1983 1985 1987 Figure 4-2. Spatial and temporal 'trends for major benthic groups In the Neosho River near Wolf Creek Generating Station. 1973-1987.

C7, 1 ' 1 -c -ý -Annual Number of Taxa WCGS Cooling Lake Tubificid Contribution to Benthos WCGS Cooling Lake 50-40'CM Loc 2 Loc 6 EM Loc a-I-0 3 2 1 30e.Total 0 -10 1981 1982 1983 1984 1985 1986 1987 I-0 0 0 Naidid Contribution to Benthos WCGS Cooling Lake 1981 1982 1983 1984 1985 1986 1987 Midge Contribution to Benthos WCGS Cooling Lake I 0 a 5O-40 30 20 Loc 2/ Loc Loc 6 a I.0 I-.I 0 4 I 0 1981 1982 1983 1984 1985 1986 1987 1981 1982 1983 1984 1985 1986 1987 Figure 4-3. Spatial and temporal trends In total taxa and major benthic groups in the Cooling Lake at Wolf Creek Generating Station, 1981-1987.

Spatial Benthic Density WCGS Cooling Lake Spatial Benthic Diversity WCGS Cooling Lake Loc 2-Loc 6 E'Z1 L=c 8 c.J 15 0 11 0 1981 1982 1983 1984 1985 1986 1987 1981 1982 1983 1984 1985 1986 1987 Seasonal Benthic Density WCGS Cooling Lake Annual Benthic Density WCGS Cooling Lake Lro-Ffl b II I L-H 2500-2000-1500-1000-500-*'7J FEB-+vAAR APR J.IN AUG OCT DEC 1981 1982 1983 1984 1985 1986 1987 Figure 4-4. Macroinvertebrate density and diversity In the Cooling Lake at Wolf Creek Generating Station, 1981-1987.

TABLE 4-1 OCCURRENCE OF MACROINVERTEBRATES AT SAMPLING LOCATIONS IN THE NEOSHO RIVER AND COOLING LAKE NEAR WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS Taxa Cnidaria Hydrozoa Hdroidae Clavidae Cordylophora lacustris Allman Hydridae Hydra sp. Linnaeus Platyhelminthes Turbellaria Tricladida Unidentified Tricladida Planariidae Dugesia sp. Ginard Nematoda Unidentified Nematoda Entroprocta Phylactolaemata Plumetellidae Unidentified Plumatellidae Urnatellidae Urnatella gracilis Leidy Annida Oligochaeta Plesiopora Enchytraeidae Unidentified Eachytraeidae Naididae Dero digitata Muller Dero trifida (Loden)Dero sp.Haemonais waldvogeli Bretcher Nais bretcheri (Michaelsen)

Nais communis Nais elinquis Muller Nais pardalis Nais simplex Piquet RiTl variabilis Piquet Nais sp. (Muller)0-hdanais serpentina Muller Pristina foreli Piquet Pristina longisoma leidyi Pristina sp.Tubi ficidae Imm. with cap. chaetae Imm. without cap. chaetae Aulodrilus limnobius Bretcher Aulodrilus piqueti Kowaleski Branchiura sower yi Beddard Iiyodrilus mastix (Brinkhurst)

Ilyodrilus temp-l-etoni Southern Limnodrilus cervix Brinkhurst Limnodrilus claparedionus Claparede Limnodrilus hoffmeisteri Claparede Neosho River 1 10 4 Cooling Lake 2 6 8 X x X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X x X X x X X X X X X X X X K X K x x X X X K X X X X X X X X X 4-24 TABLE 4-1 (Cont.)Taxa Neosho River 1 10 4 Cooling Lake 2 6 8 x x x x Limnodrilus profundicola Verril Limnodrilus udekemianus Claparede Branchiobdellidae Unidentified Branchiobdellidae Prosopora Lumbriculidae Unidentified Lumbriculidae Hirudinea Rhychobdellida Glossiphoniidae Unidentified Glossiphoniidae Immature Glassiphoniidae Actinobdella inequiannulata Moore Actinobdella triannulata Moore Placobdella multilineaia Moore Placobdella ornata Pharyngobdellida Erpobdellidae Unidentified Brpobdellidae Immature Erpobdellidae Dina microstoma Moore Arthropoda Crustacea Amphipoda Gammaridae Crangonyx sp. Bate Talitridae Hyallella azteca (Saussure)

Decapoda Astacidae Unidentified Astacidae Orconertes virilis Hagen Orconertes sp.Plaemoniidae Palaemonetes Kadiakensis Rathbun Arachnida Acarina Hydracarina Unidentified Hydracarina Insecta Collembola Isotomidae Isotomurus sp. Barner Ephemeroptera Siphlonuridae Siphlonurus sp. Eaton Baetidae Unidentified Baetidae Baetis sp. Walsh Ohgon-euri idae Isonychia sp. Eaton Heptageniidae Unidentified Heptageniidae Immature Beptageniidae Heptagenia flavescens Walsh Heptagenia bebe McDunnough x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x/ )q TABLE 4-1 (Cont.)Taxa Heptagenia sp. Walsh Stenacron interpunctatum Jensen Stenacron sp.Stenonema inte rum McDunnough Stenonema pulchellum Walsh Stenonema terminatum Walsh Stenonema tripunctatum Banks Stenonema sp. Traver Tricarythidae Tricarthodes sp. Ulmer Caenidae Caenis sp. Stephens Patomanthidae Potamanthus myops grp.Potamanthus sp. Piclet Ephemeridae Hexagenia limbata (Serville)

Hexg a sp. (Walsh)Poyi tarcyidae Ephoron album Say Ephoron sp. Williamson Tortopus pimus Tortopus sp. McCunnough Odonata Zygoptera Unidentified Zygoptera Gomphidae Erpetogomp hus sp.Gomphus sp. Leach Macromiidae Macromia illinoiensis Walsh Macromia sp.Libellulidae Sympetrum sp. Newman Coenagrionidae Unidentified coenagrionidae AKrIa apicalis Argia moesta Hagen ATrgia TITblrallis Argia sp. Rambur Plecoptera Perlidae Unidentified Perlidae Acroneuria sp. Newman Neoperla clymene Newman Perlesta placida Hagen Hempitera Gerridae Metrobates sp. While Rheumatobates sp. Bergroth Trepobates sp. Uhler Corixidae Unidentified corixidae Megaloptera Sialidae Sailis sp. Latreille Neosho River 1 10 4 Cooling Lake 2 6 8 X X X X X X X X X X X X X X X X X X X X X X X X X x X X X X X X X X X X X X X X X X X X X X X X X X X X x x x x X X X X X X X X X X X X X X X X X X X X X X x 4-26 TABLE 4-1 (Cont.)Taxa Neosho River 1 10 4 Cooling Lake 2 6 8 Corydalidae Corydalus cornutus Linnagus Trichoptera Polycentropodidae Unidentified Polycentropodidae Cyrnellus fraternus Banks Cyrnellus sp. Banks Neuroclipsis sp. Melachlan Hydropsychidae Unidentified Hydropsychidae Cheumatopsyche sp. Wellengren Hydropsyche bidens Ross H ropsyc e orris Ross Hydropsche sTl-J-'ans Ross Hydropsyche sp.Potamyia flava Hagen Hydroptiliidae Hydroptila sp. Delman Limnephilidae Limnephilus sp. Leach Pycnopsyche sp. Banks Leptoceridae Unidentified Leptoceridae Ceraclea sp. Stephens Nectopsyche candida Hagen Nectopsyche sp. Muller Oecetis sp. McLachlan Coleoptera Unidentified Coleoptera Gyrinidae Unidentified Gyrinidae Dineutus sp. Macleay Gyretes sp.Gyrinus sp. Muller Dytiscidae Unidentified Dytiscidae Hydrophilidae Unidentified Hydrophilidae Tropisternus sp. Erichson Elmidae Unidentified Elmidae Stenelmis sp. Dufour Diptera Tipulidae Unidentified Tipulidae Hexatoma sp. Latreille Chaoboridae Chaoborus albatus (Jobas)Chaoborus punctipennis (Say)Ceratopogonidae Unidentified Ceretopoganidae Simuliidae Unidentified Simuliidae Prosimulium sp Rouband Simulium sp. Latreille Chironomidae x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x K x x x x x x x x K K K K x K x x x x X X X x x x x x x x x X X X 1 7 TABLE 4-1 (Cont.)Taxa Neosho River 1 10 4 Cooling Lake 2 6 8 Unidentified Chironomidae Pupa Chironominae Unidentified Chironominae Unidentified Chironmini Chernovskia amphitrite Saether Chironomus sp. (Meiqen)-laopelma sp. Kieffer Cladotanytarsus sp. Kieffer Cryptochironomus sp. (Kieffer)Dicrotendepis sp. Kieffer En ochironomus sp. Kieffer Glyptotendipes lobiferus Say Glptotendipes sp. Kieffer Kiefferulus sp. Gaetghebuer Microchiranomus sp. Kieffer Microspectra sp. Kieffer Parachironomus sp. Lenz Paralauterborniella sp. Lenz Paratanytarsus sp. Kieffer Phaenopspectra sp. Kieffer Polypedi um ss convictum type (Walker)Polypedi um ss scalaenum type Schrank Po ypedi um ss simulans type Tovnes Polapedilum sp. Kieffer Pseudochironomus sp. Malloch Rheotanytarsus sp. Bause Stictochironomus sp. Kieffer Tanytarsus sp. VanderWulp Tribelos sp.Xennchironomus anceus Roback Tanypodinae Unidentified Tanypodinae Ablabesmyia sp. Johannsen Coeleotanypus coacinnus (coquillett) otanypus sp. Kieffer Labrundira a sp. Fittkan Larsia sp. Fittkau Procladius sp.Pentaneura sp. Philippi Tanypus sp. Meiqen enemanaimyia Group Fittkau Orthorladiinae Unidentified orthorcladiiae Corynoneura sp. Winnertz Cricotopus bicinctus group Merger Cricotopus fucus Kieffer Cricotopus ss intersectus Sensuhiruenaja Cricotopus tremulus group Circotopus ss tremulus type Sensuhiruenaja Cricotopus triannulatus Macquart Cricotopus vierriensis Goetghebuer Cricotopus sp. Van der Wulp Eukiefferiella sp Thieneman Hydrobaenus sp. Brundin Nanocladius sp. Kieffer Octhocladius oliveri Sapenis x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x X x x x x x x x x K x x x x x x x x x x x x x x x X X x K X x x x x X X X X X x X x X X X K x x x x x x x x K K K K x X 4-28 TABLE 4-1 (Cont.)Neosho River f1 -10 4 Cooling Lake 2 6 8 Taxa Octhocladius sp. Van Der Wulp Thienemanniella sp. Kieffer Dolichopodidae Unidentified Dolichopadidae Mollusca Gastropoda Unidentified Gastropoda Pulmonata Physidae Physa sp Draparnaud Ancylidae Unidentified Ancylidae Ferrissio rivularis (Say)Ferrissia sp. Walker Pelecypoda Heterodonata Sphaeriidae Musculium transversum Muller Pisidium sp. Pfeiffer Sphaerium transversum (Say)Spharium sp. Scopoli Corbiui dae Corbicula fluminea Muller CorbIcula sp.Unianidae Anodanta grandis Say Lampsilis ovata Say x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x K x Total Taxa 87 Combined Taxa 126 118 54 42 26 179 70 4-29 TABLE 4-2 ANNUAL NUMBER OF MACROINVERTEBRATE TAXA COLLECTED FROM LOCATIONS 1, 10, AND 4 IN THE NEOSHO RIVER AND LOCATIONS 2, 6, AND 8 IN THE COOLING LAKE OF WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS 1973-1987 I.1.:;Site/Year Neosho River 1973 1974 1975 1976 1977 1978 1979 1980 1981 1982 1983 1984 1985 1986 1987 Cooling Lake 1 10 4 Total Location/Number of Taxa 66 55 87 75 35 42 20 34 38 95 76 73 84 60 61 32 58 47 79 76 69 75 46 54 30 53 46 26 58 99 132 108 114 121 85 85 57 82 80 2 6 8 Total 1981 1982 1983 1984 1985 1986 1987 20 22 17 20 14 18 10 27 12 14 12 11 15 14 15 13 15 6 32 26 20 29 20 25 19 Notes: 1. Dash (-) indicates location not sampled.2. Asterisk (*) indicates location results not reported separately.

3. Results for river include both qualitative and quantitative samplings.

4. Ponar sampling only was conducted in the cooling lake.4-30 TABLE 4-3 MACROINVERTEBRATE DENSITY AND DIVERSITY IN PONAR COLLECTIONS FROM LOCATIONS 10 AND 4 IN THE NEOSHO RIVER STATION, BURLINGTON, KANSAS, 1973-1987 NEAR WOLF CREEK GENERATING 2/m )Density (No.Date 27MAR73 11JUN73 10SEP73 10DEC73 Mean 26MAR74 11JUN74 10SEP74 10DEC74 Mean 17APR75 10JUN75 9SEP75 3DEC75 Mean 25FEB76 6APR76 15JUN76 9AUG76 50CT76 14DEC76 Mean 22FEB77 4APR77 8JUN77 9AUG77 4OCT77 13DEC77 Mean 21FEB78 25APR78 27JUN78 29AUG78 10OCT78 12DEC78 Mean 10 4 Mean Diversity (base 2)10 4 Mean 38 265 189 164 340 2646 974 567 1132 4366 13098 350 23795 16282 16084 12329 35504 15498 265 510 14317 2485 11430 104 1606 9894 7881 4536 11179 5867 1598 143 871 142 463 1370 658 388 2174 1257 2438 1564 3657 8496 1890 7590 4328 9941 5984 8902 5746 605 1701 14723 1852 5588 718 1002 2022 1077 3449 3081 1892 1598 143 871 90 364 780 411 364 2410 1116 1503 1348 4012 10797 1120 15693 10305 13013 9156 22203 10622 435 1106 14520 2169 8509 411 1304 5958 4479 3993 7130 3879 1.90 2.05 1.01 3.36 2.08 3.78 4.32 2.31 3.38 4.05 4.27 3.69 2.27 3.74 1.89 2.49 1.83 2.69 2.49 1.88 3.43 2.36 3.54 3.18 3.18 2.93 2.23 2.67 2.41 3.47 2.70 3.30 3.76 2.10 2.92 2.62 3.58 3.05 2.85 3.54 3.33 2.66 2.09 2.31 2.80 2.39 3.62 3.09 3.03 2.87 3.71 3.12 2.07 2.36 1.71 3.42 2.39 3.54 4.04 2.21 3.15 3.34 3.93 3.37 2.56 3.64 2.61 2.58 1.96 2.50 2.64 2.14 3.53 2.73 3.29 3.03 3.45 3.02 4-31 1'U'V TABLE 4-3 (Cant.)2 Density (No./m)10 4 Mean Diversity (base 2)10 4 Mean Date I., I--20FEB79 10APR79 12JUN79 6AUG79 8OCT79 10DEC79 Mean 15APR80 17JUN80 28OCT80 16DEC80 Mean 27APR81 22JUN81 19OCT81 15DEC81 Mean 514AR85 16APR85 22JUL85 23SEP85 19NOV85 9DEC85 Mean 3MAR86 28APR86 22JUL86 19AUG86 17NOV86 16DEC86 Mean 2MAR87 28APR87 22JUN87 24AUG87 Mean 30051 775 576 869 7532 57 6643 4413 17360 435 7403 8713 1975 66 9 2691 28 94 266 0 0 0 65 142 227 1852 113 19 9 394 1783 226 406 123 635 3270 633 983 813 3307 94 1517 1399 4111 567 2026 6880 3478 2750 76 3296 38 66 0 9 28 28 236 76 605 463 473 371 1311 208 151 245 479 16661 704 780 841 5420 76 4080 2906 10736 501 4714 7797 2727 1408 43 2993 33 80 266 0 5 14 48 142 232 964 359 241 241 383 1547 217 279 184 557 4.00 3.57 2.13 2.61 3.94 1.29 2.92 3.98 4.19 3.25 3.81 3.86 3.00 1.61 2.12 0.92 2.32 1.64 0.81 1.87 2.70 1.27 1.83 1.00 1.45 2.95 1.83 1.25 1.91 1.99 2.71 3.79 3.10 3.02 3.10 2.13 2.98 3.23 4.07 2.38 3.23 2.84 3.04 2.80 1.79 2.62 1.50 2.81 1.58 1.18 2.47 0.00 3.39 2.25 2.48 2.12 3.51 1.30 1.50 1.72 2.01 3.36 3.68 2.62 2.82 3.52 1.71 2.95 3.61 4.13 2.82 3.52 3.35 3.02 2.21 0.90 2.37 1.21 2.57 1.64 0.00 0.00 0.79 0.98 1.87 2.59 0.64 2.61 1.63 1.24 1.75 2.32 1.57 1.38 1.82 2.00 Notes: 1. Dash (-) indicates locations not sampled or diversity not calculated.

2. Asterisk (*) indicates diversity not calculated because <2 organisms collected.

Diversity assumed to be 0.00 for mean calculations.

4-32 TABLE 4-4 SELECT MACROINVERTEBRATE TAXA DENSITIES IN PONAR COLLECTIONS FROM LOCATIONS 10 AND 4 IN THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1973-1987 Dtigochaetes Mayflies Stoneflies Caddisflies 0idges Date 10 4 10 4 10 4 10 4 0 _4 27MAR73 .....- -.11JUN73 ----------10SEP73 906 38 -416 10DEC73 20 38 -19 Mean 463 38 -218 26MAR74 ----------11JUN74 9 0 0 38 0 0 0 0 9 38 10SEP74 0 350 113 85 0 0 57 0 38 28 10DEC74 19 737 19 19 0 0 19 19 94 576 Mean 9 362 44 47 0 0 25 6 47 214 17APR75 218 38 0 19 0 0 0 114 86 104 10JUN75 28 10 264 567 19 0 1200 1163 1012 369 9SEP75 19 28 19 76 56 454 832 520 10 57 3DEC75 38 237 57 227 10 10 19 57 246 1664 Mean 76 78 85 222 21 116 513 464 339 549 25FEB76 397 217 57 132 38 1172 283 38 3147 1285 6APR76 2982 2675 142 160 775 671 1105 19 6709 4593 15JUN76 28 0 151 1200 0 66 66 321 57 113 9AUG76 47 245 1624 3676 737 85 18919 19 2240 3307 50CT76 85 408 2930 312 225 38 3496 0 8533 3393 14DEC76 387 822 3827 672 1011 9 4403 75 4876 7475 Mean 639 728 1455 1025 464 340 4712 79 4260 3361 ,22FEB77 567 94 973 274 0 38 3034 9 29286 8108 4APR77 3374 1342 396 37 9 9 56 0 11000 3884 8JUN77 113 160 9 95 0 0 9 28 57 113 9AUG77 255 47 122 1125 9 47 0 311 104 151 40CT77 56 141 302 841 246 208 12748 12748 652 756 13DEC77 85 38 9 37 9 76 1427 1228 539 217 Mean 742 304 302 402 46 63 2879 2387 6940 2205 21FEB78 28 85 0 19 0 9 38 482 38 104 25APR78 37 19 19 104 57 66 584 208 463 208 27JUN78 94 312 1029 916 19 19 7862 0 803 576 29AUG78 19 123 1654 19 9 0 793 28 5207 888 10OCT78 378 529 1143 1143 151 0 28 19 2693 2164 12DEC78 491 9 321 132 9 813 28 18 10140 1673 Mean 175 180 694 389 41 151 1556 126 3224 936 4-33 TABLE 4-4 (Cont.)OliDochaetes Mayflies Stoneflies Caddisflies Midges Date 10 4 i0 4 I__ 4 10 4 10 4 I0 4 20FEB79 5075 1739 1522 95 850 9 3534 28 15329 1304 10APR79 132 151 47 28 0 0 0 9 472 274 12JUN79 19 538 47 0 0 0 0 0 170 180 6AUG79 19 19 236 236 0 0 425 265 161 142 8OCT79 19 85 435 179 66 142 1370 189 5358 2665 10DEC79 9 19 0 9 0 0 9 19 28 47 Mean 879 425 381 91 153 25 890 85 3586 769 15APR80 ----------17JUN80 0 0 397 350 265 47 1531 444 1909 519 28OCT80 331 472 7173 832 1210 66 5320 19 1682 1918 16DEC80 227 397 38 47 47 0 9 0 28 98 Mean 186 299 2536 410 507 38 2287 154 1206 845 27APR81 435 265 1691 76 123 0 217 0 5887 6067 2JUN81 47 208 1134 2117 0 0 113 208 444 766 19OCT81 0 274 19 47 0 2 9 28 19 2202 15DEC81 9 28 0 0 0 0 0 0 0 28 Mean 123 194 711 560 31 1 85 59 1588 2266 5MAR85 0 19 0 0 0 0 0 1 9 0 16APR85 9 28 0 0 0 0 0 0 66 238 22JUL85 0 0 170 -23SEP85 0 0 0 0 0 0 0 0 0 0 19NOV85 0 0 0 0 0 0 0 9 0 0 9DEC85 0 9 0 0 0 0 0 9 0 9 Mean 2 11 8 0 0 0 0 4 41 9 3MAR86 19 0 104 -28APR86 9 19 9 9 19 47 113 9 9 9 22JUL86 0 0 95 76 161 0 1588 0 9 0 19AUG86 76 95 0 38 0 104 0 170 28 95 17NOV86 0 9 0 0 0 38 0 151 0 9 ,16DEC86 0 0 0 0 0 57 0 198 0 0 Mean 17 25 17 25 30 49 285 106 25 23 2MAR87 18 349 104 38 76 0 679 76 613 679 28APR87 9 9 9 0 9 0 19 19 0 9 22JUN87 0 0 28 28 9 19 349 104 0 0 24AUG87 0 0 0 0 9 9 94 38 9 0 Mean 7 90 35 17 26 7 285 59 156 172 Notes: 1. Dash (-) indicates location not sampled.4-34 TABLE 4-5 SELECT MACROINVERTEBRATE TAXA DENSITIES IN PONAR COLLECTIONS FROM LOCATIONS 2, 6, AND 8 IN THE COOLING LAKE OF WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1981-1987 Naidadae Tubificide Midges Other Date 2 6 8 2 6 8 2 6 8 2 6 8 23FEB81 28APR81 23JUN81 24AUG81 20OCT81 15DEC81 Mean 3MAR82 27APR82 22JUN82 31AUG82 19OCT82 7DEC82 Mean IMAR83 25APR83 23JUN83 29AUG83 18OCT83 13DEC83 Mean 29FEB84 17APR84 19JUN84 21AUG84 16OCT84 17DEC84 Mean SMAR85 16APR85 24JUN85 26AUG85 21OCT85 9DEC85 Mean 3MAR86 28APR86 24JUN86 19AUG86 27ocT86 16DEC86 Mean 2MAR87 28APR87 22JUN87 24AUG87 Mean 0 38 369 680 272 331 28 19 860 9 274 254 9 0 0 38 0 0 8 0 189 9 0 387 151 123 19 0 0 0 208 47 46 0 19 0 0 0 0 3 0 0 0 0 9 0 2 0 0 0 0 0 0 0 9 0 0 0 0 0 2 9 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 9 0 0 0 0 3 0 0 0 0 0-208 1162 57 3667 1625 302 19 0 0-85 1283 332 0 0 0 813 0 0 0 473 66 1711 38 1985 17 830 95 217 66 1143 28 76 113 680 85 19 19 85 68 370 217 9 95 331 9 28 66 217 28 9 85 95 83 115 19 302 198 2863 0 0 0 0 28 9 0 0 41 529 47 161 0 9 0 57 19 113 9 104 9 47 14 82 0 28 0 925 0 38 0 0 0 248-0-142-66-132-85-6483-1682-189-132-577-1040-1684-633-567-142-47-19-38-241 605 170 66 19 38 19 76 227 19 510 47 756 142 284 9 161 95 454 0 19 38 85 9 113 19 66 28 150 180 680 28 76 47 28 47 151 142 85 38 104 80 187 47 208 19 123 0 28 0 66 17 106 766 38 142 142 28 28 191 104 104 9 0 255 312 131 28 482 142 85 0 9 124 19 28 19 28 24 28 9 0 0 19 47 17 39 9 0 0 0 0 8 10 19 312 9 0 0 58 0 0 0 0 0 0 0 9 18 0 0 0 0 5 19 312 85 435 38 28 9 9 0 85 113 246 44 186 142 104 265 548 57 95 0 38 0 0 113 57 96 140 113 624 104 104 19 0 0 19 19 142 9 38 44 155 0 0 0 19 9 47 13 9 9 0 0 0 0 3 0 28 0 0 0 0 5 0 0 0 9 0 0 2 0 0 0 0 0 0 0 0 38 0 0 0 0 6 0 19 0 0 0 0 3 123 0 0 0 19 0 24 104 66 0 0 43 47 19 9 19 9 19 20 28 9 9 0 12 0 0 9 0 28 0 6 66 9 0 0 19 76 57 19 19 43 38 38 0 0 19 0 0 0 0 0 Note: 1. Dash (-) indicates location not sampled.4-35 ki Yr TABLE 4-6 MACROINVERTEBRATE DENSITY AND DIVERSITY IN PONAR COLLECTIONS FROM LOCATIONS 2, 6, AND 8 IN THE COOLING LAKE OF WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1981-1987 2 Density (No./m 2 6 8 Mean Date 23FEB81 28APR81 23JUN81 24AUG81 20OCT81 15DEC81 Mean 3MAR82 27APR82 22JUN82 31AUG82 19OCT82 7DEC82 Mean 1MAR83 25APR83 23JUN83 29AUG83 18OCT83 13DEC83 Mean 29FEB84 17APR84 19JUN84 21AUG84 16OCT84 17DEC84 Mean 5MAR85 16APR85 24JUN85 26AUG85 21OCT85 9DEC85 Mean 3MAR86 28APR86 24JUN86 19AUG86 27OCT86 16DEC86 Mean 2MAR87 28APR87 22JUN87 24AUG87 Mean Diversity (base 2)2 6 8 Mean 1181 3884 775 898 1685 6936 1729 208 1068 671 1399 2002 746 643 170 217 104 57 323 387 113 28 359 586 907 397 189 662 19 85 151 66 195 784 94 38 189 113 142 227 245 132 38 66 120 992 302 2088 208 416 274 713 198 917 9 472 2202 2438 1039 255 1673 217 765 38 113 510 38 454 66 227 9 217 169 442 3128 57 0 9 113 625 406 113 94 113 170 76 162 274 1057 57 19 352 964 501 66 94 113 293 339 113 680 94 76 9 76 175 822 161 47 66 293 76 244 85 57 9 0 38 992 742 2986 492 657 274 1102 3567 1323 109 770 1437 1919 1521 501 1158 194 491 71 85 417 463 356 53 227 236 472 301 248 1490 57 54 56 85 332 671 123 60 123 192 98 211 201 415 35 28 170 0.75 1.55 2.36 1.57 1.56 2.37 2.19 1.48 1.07 1.94 2.97 2.00 1.97 2.22 1.40 2.77 1.31 0.92 1.77 2.57 1.38 0.92 3.24 0.83 1.93 1.81 1.12 2.64 0.00 0.50 2.00 0.59 1.14 1.97 1.57 1.50 1.98 1.21 1.74 1.66 2.25 1.20 1.50 1.38 1.58 2.92 2.36 1.86 1.34 0.73 2.14 1.89 2.03 1.57 0.86 1.84 1.56 1.31 1.43 1.98 1.56 0.79 1.00 1.78 1.42 1.50 2.31 0.99 0.74 2.35 1.32 2.15 2.20 0.92 ft 0.00 0.88 2.73 1.28 2.12 0.65 2.59 1.75 1.85 2.66 2.31 0.92 1.00 1.72 2.39 1.68 0.99 1.57 1.21 1.70 1.59 0.41 2.23 1.30 1.91 1.07 1.15 2.48 2.02 0.00 0.86 2.33 1.00 1.45 2.20 1.58 0.95 2.92 1.56 1.71 1.85 1.15 2.14 1.76 2.20 1.88 0.74 0.87 1.89 2.27 1.66 1.70 2.10 1.48 1.78 1.16 1.35 1.59 2.15 1.79 0.97 1.85 0.68 1.99 1.57 1.23 2.36 0.74 0.80 0.67 0;55 1.06 2.39 1.62 1.21 1.16 2.04 1.50 1.65 2.37 1.70 0.81 0.79 1.42 Notes: 1. Dash (-) indicates location not sampled.2. Asterisk (*) indicates diversity not calculated because <2 organisms collected.

Diversity assumed to be 0.00 for mean calculations.

4-36 TABLE 4-7

SUMMARY

OF ASIATIC CLAM (CORBICULA FLUMINEA)

ABUNDANCE IN PONAR GRABS FROM TWO LOCATIONS ON THE NEOSHO RIVER, BURLINGTON KANSAS Location 4 No. Density (No./m )Location 10 No. Density (No./m )Date AUG 86 NOV 86 DEC 86 MAR 87 APR 87 JUN 87 AUG 87 Mean 6 5 5 6 2 0 17 56.7 47.3 47.3 56.7 18.9 0.0 79.9 43.8 1 0 1 2 1 1 1 9.4 0.0 9.4 18.9 9.4 9.4 9.4 9.4 Note: Density data represents mean of duplicate samples from each location.4-37 TABLE 4-8

SUMMARY

OF SAMPLES COLLECTED DURING SURVEY FOR ASIATIC GENERATING STATION, 30 SEPTEMBER

-1 OCTOBER 1987 CLAMS IN THE VICINITY OF WOLF CREEK Site WCCL Location/Replicate CWSH 1 2 3 4 5 6 7 Water Temperature (C)21.3 Water Depth (ft.)4 4 5 5 15 18 6 Gear(a) Substrate Type(b)No. of Asiatic clams P Clay, gravel P Clay, gravel P Ooze, clay P Ooze, clay, gravel, rock P Silt, gravel P Clay, gravel, rock P Ooze, detritus, Fine gravel P Ooze, detritus, Fine gravel 8 WCCL MUDS 1 2 3 4 5 6 7 23.7 6 7 5 3 4 2 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 P Silt, clay, P Silt, clay, P Silt, clay, P Silt, clay, P Silt, clay, P Silt, clay, P Silt, clay, Gravel P silt, clay, Gravel detritus detritus detritus detritus detritus detritus detritus, detritus, Is WCCL Service Spillway 1 2 3 4 Gravel Beach 1 2 WCCL Wolf-Creek FAS-10 Bridge 1 2 3 23.1 23.1 21.5 20.5 3 3 4 4 1-2 1-2 1-2 P P P P Detritus, silt, clay Detritus, silt, clay Detritus, silt, clay Detritus, silt, clay R Upland gravel R Upland gravel R Upland gravel 0 0 0 0 0 0 0 Neosho River Burlington Dam Downstream 1 2 3 4<1 (1 (1 U'I U R R R PP PP PP PP Silt, gravel Silt, gravel Silt, gravel Gravel, sand, silt Gravel, sand, silt Gravel, sand, silt Gravel, sand, silt 0 0 0 1 1/2 1/2 TABLE 4-8 (Cont.)Site Neosho River Location/Replicate MUSH Chute below MUSH Chute below Island-and Stilling Basin U.S. 75 Bridge Upstream 50 m At bridge Downstream-150 m 200 m 300 m Hartford Boat Ramp Downstream Upstream 1 2 3 4 5 6 7 8 9 10 Water Temperature (C)Water Depth (ft.)Gear(a) Substrate Type(b)No. of Asiatic clams 19.7 19.5 19.5 19.6 19.1 19.1<1-3 (1-2 B 1 1 4 3 6 5 4 S, R Bedrock, gravel, silt S, R Gravel, bedrock P Gravel P Clay P Clay P Clay P Detritus, silt P Detritus, silt P Clay, woody debris P Clay, woody debris p Silt, detritus P Silt, clay p Silt, clay P Gravel, rock, silt P Gravel, rock, silt R Gravel, rock, silt P Silt, clay S Silt, gravel S Gravel S Gravel S Gravel 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 Neosho River 41 2 2 2 1 1-2 3 2 1-3 1-3 1-3 (a) Gear: P=ponar, PP=petite ponar, R=clam rake, S=seine.(b) Substrate types listed in order of dominance.

5. FISHERIES

5.1 INTRODUCTION

Adult, juvenile, and larval fish were monitored in the Neosho River and Wolf Creek to provide data on potential construction and operational impacts of WCGS on the aquatic communities since 1973. Baseline information was obtained from the tailwaters of John Redmond Reservoir (JRR) to determine potential impingement and entrainment losses at the make up water screenhouse.

Areas upstream and downstream of the Wolf Creek and Neosho River confluence were monitored to determine potential effects of construction and dam closure. The occurrence of fishes in Wolf Creek was monitored to (1) establish seasonal and spatial patterns, (2) monitor construction effects, and (3) to establish the value of Wolf Creek as a spawning and/or nursery area.This report section summarizes adult and juvenile data collected from the Neosho River from 1973 through 1982 and 1985 through August 1987. The data represent the seining and electrofishing program that provided a basis for preoperational-operational comparisons.

Data from program components that were not incorporated into the operational study initiated in 1985 (EA 1986) were not included in this report. Preimpoundment fisheries data from Wolf Creek were obtained through 1980 and results are summarized in annual monitoring reports and the WCGS environmental report (KGE 1981). Results of post-impoundment cooling lake fisheries studies are presented in annual reports prepared by Ecological Analysts (1983) and WCNOC (1988).5-1 5.2 FIELD AND ANALYTICAL PROCEDURES Surveys of the Neosho River from the tributaries of JRR to below the Wolf Creek confluence were conducted seasonally from 1973 through 1982 and 1985 through 1987. Locations and gears changed during early study years. Location 1 was in John Redmond Reservoir upstream of the dam until 1977 when the tailwaters location replaced the reservoir location.

Gill and hoop nets were also part of the program but were discontinued when the reservoir location was replaced in 1977 and when electrofishing proved more effective than hoop nets to determine species composition and relative abundance in the Neosho River. Locations were added in 1975 to include Location 10 upstream of the confluence with Wolf Creek and Location 11, a riffle immediately below the confluence that was monitored only for Neosho madtom.Data collected from 1977 through 1981 and 1985 through 1987 were most comparable because of the above changes. Differences between these data sets, which essentially represent preoperational-operational data are summarized in Table 5-1. Program changes were made as milestones were met at WCGS. The actual sampling scheme reflects schedule changes resulting from weather and water conditions.

Cold weather often resulted in sampling in early March rather than February, and high water conditions occasionally caused sampling to be delayed or missed.An AC boat-mounted boom shocker has been used since 1977 to collect fish at Locations 1, 10, and 4. Sampling consisted of a 30-minute effort at each location and encompassed approximately 800 meters of shoreline.

Changes in the electrofishing equipment used during the study probably contributed to lower 5-2 catches in recent years. The three-phase 230 volt AC generator used from 1977 through 1980 was replaced by a 3,000 watt single-phase generator in 1981. From 1982 through 1987, a single-phase 3,500 watt generator was used with a Coffelt model UVP-15 unit. An operating comparison (Heidinger et al. 1983) of three electrofishing systems similar to those used in this study indicate substantial differences in efficiency with the Coffelt unit the least efficient.

The difference between the Coffelt and the Homelite systems (which were very similar to that used in the Neosho River from 1977-1980) was attributed to a combination of electrode array and amperage drawn. The electrode array for the Neosho River study remained the same, although the electrodes were changed in 1981.A 1.8 x 4.6 meter straight seine with 0.3 centimeter Ace mesh was used to collect forage-sized fish from shallow areas at Locations 1, 10, and 4 on the Neosho River. Generally two to four straight seine hauls were performed at each location when water levels were suitable.

Since 1976 qualitative kick seines have been performed at Location 11 using the standard seine to document the presence of the Neosho madtom, listed as threatened in Kansas (Kansas Administrative Regulations, 1987). All Neosho madtoms collected using this method were measured and released at the time of capture.Fish collected by electrofishing were identified, weighed, measured, and released alive unless needed for radiological/environmental samples. Seine samples (excluding Neosho madtom) were preserved in 10 percent formalin and returned to the laboratory for analysis.

Each fish collected was identified, measured, and weighed (if greater than 10 g). Species represented by more than 25 individuals were counted after 25 lengths were recorded.5-3 Catch per unit effort (CPE) was defined as the number of fish collected per 30 minutes (No./30 min.) of electroshocking.

Seine catches were presented as the number of fish collected per seine location (excluding Location 11). Spatial and temporal comparisons were based on CPE data.5.3 RESULTS AND DISCUSSION 5.3.1 Overview Surveys of the Neosho River from 1973 through 1987 (exclusive of 1983 and 1984)from the tailwaters of John Redmond Reservoir (JRR) to below the confluence with Wolf Creek yielded 52 species representing 12 families (Table 5-2).Annual surveys encountered 29 to 41 species, with 13 species reported during each of the 13 years, including gizzard shad, carp, golden shiner, ghost shiner, red shiner, river carpsucker, smallmouth buffalo, channel catfish, white bass, green sunfish, orangespotted sunfish, white crappie, and freshwater drum. Nine additional species occurred during 11-12 annual surveys, and four (shorthead redhorse, blue suckers, flathead catfish, and mosquitofish) were collected each year after electrofishing was initiated in 1977 (Table 5-2).Similarly, Neosho madtom were encountered each year except 1978 after kick seining at Location 11 was added to the program. All reported species from the study area are common to the Neosho River system except wiper and walleye which were introduced through stocking activities of the Kansas Department of Parks and Wildlife.5-4 Electrofishing and seine catches from 1977 -1982 and 1985 -1987 were utilized to examine species composition and relative abundance of the Neosho River fish community.

The combined gear provided the best representation of species encountered in the study. The total catch of 35,400 fish captured during the 9 years that both gears were used was dominated by cyprinids and herrings (i.e.gizzard shad), which accounted for 65 and 17 percent, respectively.

Comparison of preoperational (1977-82) and operational (1985-87) data sets indicated only slight shifts in relative abundance at the family level: RELATIVE ABUNDANCE 1977-82 1985-87 Family (Preoperational) (Operational)

Gars 0.3 0.3 Herrings 16.4 16.8 Minnows/Carp 61.2 73.0 Suckers 7.8 2.0 Catfishes 3.1 1.4 Topminnows

<0.1 <0.1 Livebearers 0.8 2.7 Silversides 0.4 0.1 Temperate basses 1.7 0.4 Sunfishes 4.6 2.0 Perches 0.3 0.2 Drums 3.5 1.0 5-5 The greatest difference between data sets occurred for cyprinids which increased by nearly 12 percent. That increase primarily reflected lower relative abundance for all families except gars, herrings, topminnows, and perches which were relatively unchanged and livebearers which increased.

The increase of livebearers was due to a large 1987 catch of mosquitofish (317 fish) that accounted for 61 percent of the total 9-year catch for these fish.Thirteen species contributed 83 to 97 percent to the annual catches and accounted for 95 percent of the total catch for the 9 years that both electrofishing and seining were conducted.

Annual differences were apparent at the species level, although red shiner was the most abundant species all years except 1982 (Table 5-3). The 1982 survey included only tailwater collections where red shiner catches were typically low (Section 5.3.3). Gizzard shad were codominant except in 1980 and from 1985-1987 when ghost shiner catches were higher. Approximately 74 percent of the ghost shiner captured in the 9-year study were collected during the operational study period.5.3.2 Electrofishing Electrofishing in the Neosho River from 1977-1982 and 1985-1987 captured 7,918 fish representing 37 species and two hybrids (Table 5-4). Gizzard shad accounted for 35 percent of the total electrofishing catch and was the most abundant species except in 1979 and 1980 when river carpsucker catches were highest. River carpsucker accounted for 13 percent of the total electrofishing catch and other catostomids as a group contributed 10 percent. Few catostomids were collected by seining (Section 5.3.3) and all blue sucker were taken by electrofishing.

Blue sucker were considered a threatened fish in Kansas until 5-6 May 1, 1987 when it was removed from the list (Kansas Administrative I-.LP Regulations, 1987).Total catch per unit effort (CPE) ranged from 39.0 to 90.1 during the 1977-82 preoperational study, compared to 16.1 to 38.5 during the 1985-87 operational study (Table 5-4). Changes in electrofishing equipment after 1980 (Section Ii! 5.2) likely contributed to lower CPE in recent years which averaged 40.5 after the change compared to 70.9 from 1977-80. The low catch in 1980 before the equipment change was due primarily to the lowest annual catch of gizzard shad and relatively low catches of white bass and white crappie (Table 5-4).Catches of all three species are typically highest at Location 1 in the JRR tailwaters and appear related to year-class strength in John Redmond Reservoir and seasonal releases from JRR dam.Relative abundance and the average CPE for predominant species in the electrofishing catch declined during the operational study except for increased relative abundance of gizzard shad, smallmouth buffalo, and flathead catfish (Table 5-5). Overall operational catches averaged 55 percent lower than the preoperational average with percent reductions ranging from 34 (gizzard shad)to 82 percent (white bass). Catch rates of flathead catfish were similar between preoperational and operational study periods because of high catches in 1986 and 1987 that ranked second and third for the 9-year study (Table 5-4).Consistent spatial differences were apparent throughout the 9-year study with higher total electrofishing catches from the JRR tailvaters (Location 1), followed by Location 10 upstream of the confluence with Wolf Creek (Table 5-6).Catch rates below the Wolf Creek confluence were consistently lower except in 5-7 1981 and 1987 when average CPE was slightly higher than at Location 10. Higher catches from the JRR tailwaters resulted primarily from catches of gizzard shad, although average catch rates of most other predominant species were also higher in the tailwaters than in the lower river. Exceptions included freshwater drum, carp, and flathead catfish. Freshwater drum and carp catches averaged highest upstream of the Wolf Creek confluence (Location 10), whereas flathead catfish catch rate averaged highest below the confluence (Location 4).Annual catches generally reflected the overall spatial differences as there were only 17 exceptions (7 percent), primarily for carp, smallmouth buffalo, and flathead catfish, species with relatively low average catch rates.Higher catches at Location 1 have been attributed to habitat differences between the tailwaters and the lower river locations and to the proximity of Location 1 to JRR. Several species (e.g., gizzard shad, white bass, and white crappie) are thought to originate primarily from JRR. Catch rates for the ten predominant species, as a group, averaged 61 percent higher upstream of the confluence with Wolf Creek than at the downstream sampling site. Catches between the two lower locations were most similar in 1981 and 1987 (Table 5-6).Habitat differences also existed between Locations 10 and 4 and probably contributed to differences in catches from the lower river. Location 10 includes a large riffle and gravel bar with a long shallow run downstream of the riffle, whereas Location 4 is on a bend in the river below the riffle at Location 11 and includes a deep pool and smaller gravel bar.5-8 5.3.3 Seining Neosho River seine collections from 1973-1982 and 1985-1987 captured 36,306 fish. Collections were dominated by gizzard shad, ghost shiner, and red shiner, which collectively accounted for over 90 percent of the seine catches during the 12-year study (Table 5-7). Annual catches of red shiner ranked first except in 1973, and catches of gizzard shad and ghost shiner generally ranked second or third. Data from 1973-1975 included seine collections only below the confluence with Wolf Creek (Location 4), whereas after 1975 three sites (Locations 1, 10, and 4) were surveyed.

The occurrence of Neosho madtom after 1975 resulted from the addition of qualitative kick seining at a riffle immediately below the Wolf Creek confluence (Location 11), although subsequent sampling at Locations 10 and 4 also yielded Neosho madtom. The numbers of fish per seine collection did not exhibit a long-term trend. The lowest catch occurred in 1973 and the highest in 1985 when WCGS began operation.

Total catches during the 1973-82 preoperational study ranged from 207 to 5,944, compared to 1,956 to 7,314 during the 1985-87 operational study.Preoperational catches from 1976-82 were compared to the operational catches to evaluate potential shifts in species composition.

Operational catches averaged 29 percent higher, due primarily to higher catches of ghost shiner (Table 5-8).Most other predominant species increased only slightly, although mosquitofish increased more than 3-fold because of a large catch in 1987 at the lower river locations (Table 5-9). The game fish catch declined by nearly 35 percent during the operational study. This group included nine species (black bullhead, channel catfish, flathead catfish, white bass, bluegill, spotted bass, largemouth bass, white crappie, and freshwater drum) but only contributed 5-9 three percent to the total catch from 1976-1982 and 1985-1987.

Bullhead minnow catches declined by 82 percent during operational study. Lower bullhead minnow catches were apparent both upstream and downstream of the Wolf Creek confluence with nearly identical reductions between preoperational and operational catch rates (Table 5-9). Bullhead minnow occurred in the JRR tailwater collections only in 1980 and 1985.Seine catches of the predominant species were highest at the lower river collection sites except for catches of gizzard shad and game fish which averaged highest from the JRR tailwaters (Location 1; Table 5-9). As was indicated for the electrofishing data, the abundance of gizzard shad, white bass and white crappie at Location 1 suggest tailwater catches reflect the influence of releases from JRR reservoir.

Cyprinid catches were much higher at the downstream sites, although ghost shiner catches at Location 1 ranked higher than upstream of the Wolf Creek confluence at Location 10. Ghost shiner collected from Location 1 were taken from a small shallow (<2 ft.) cove adjacent to the main channel leading from the JRR stilling basin, habitat apparently preferred by ghost shiner (Cross 1967).As a group, cyrpinids comprised over 90 percent of the seine catch from the lower river, compared to 46 percent of the tailwaters.

Habitat at the lower river locations include gravel bars, pools, and riffles (during normal flow)which are preferred by most cyprinid species. Juveniles of fish which are thought to originate primarily from JRR occurred most often in the tailwaters.

Red shiner have generally been the most abundant species in the Neosho River, probably because it is very adaptable to environmental variation (Cross 1967, Pflieger 1975) which may give it a competitive advantage over other cyprinid 5-10 species.5.4

SUMMARY

AND CONCLUSIONS The fish community in the Neosho River at the John Redmond Reservoir (JRR)tailwaters, and above and below the confluence with Wolf Creek has been monitored since 1973. The study was curtailed in 1981 and discontinued from 1982-1984 before reinstatement in 1985 to coincide with start up of UCdS.Potential operational effects of WCGS on the fishery were limited to diversion of water from JRR tailwaters for raw water and/or makeup water for the WCCL and the effect discharges from the WCCL would have downstream of the confluence with Wolf Creek. Following initial lake filling in 1981, maximum diversion of river water occurred 2-11 August 1987 when use of two make-up water pumps diverted 100 cfs, which was equivalent to 40 percent of the mean daily discharge from JRR during this period. Maximum diversion of river water based on mean monthly flows also occurred in August 1987 (3.1 percent) and was higher than the previously observed maxima. Closure of the WCCL dam eliminated flood stage flows in Wolf Creek and generally improved the water quality.Trends in electrofishing and seining data between locations upstream and downstream of the Wolf Creek confluence suggested changes in Wolf Creek due to the WCCL and operation of WCGS had no effects on the Neosho River fishery.Overall, few long-term trends were apparent and annual differences were related to natural variability, releases from JRR, and river flows which influenced gear efficiency.

Changes in electrofishing gear that occurred in 1981 contributed to lower catches during the operational study. Catch data did not reflect potential influences of commercial fishing in 1980, impingement losses 5-11 at the WCGS makeup water screenhouse in 1981, or a documented fish kill in August in 1986.5-12

5.5 REFERENCES

Cross, F.B. 1967. Handbook of Fishes of Kansas. Univ. Kansas Publ, Museum of Natural History. No. 45. 357 pp.EA Engineering, Science, and Technology, Inc. 1986. Wolf Creek Generating Station Operational Phase Environmental Monitoring Program, March-December 1985. Report to Kansas Gas and Electric Company, Wichita.Ecological Analysts, Inc. 1983. Wolf Creek Generating Station Environmental Monitoring Program, February 1982 -December 1982. Report to Kansas Gas and Electric Company, Wichita.Heidinger, R.G., D.R. Helms, T.I. Hiebert, and P.H. Hove. 1983. Operational comparison of three electrofishing systems. North Amer. Journal Fisheries Management 3(3): 254-257.Kansas Gas and Electric Company. 1981. Wolf Creek Generating Station Environmental report (operating license stage). Wichita, Kansas. 2 vols.Loveless, B.S. 1988. Wolf Creek Generating Station 1987 Operational Fishery Monitoring Report. Environmental Management Group, Wolf Creek Nuclear Operating Corp., Burlington, Kansas. 72 pp.Pflieger, W.L. 1975. The Fishes of Missouri.

Missouri Conservation Department, Jefferson City. 451 pp.Robins, C.R., Chairman.

1980. A List of Common and Scientific Names of Fishes from the United States and Canada. 4th edition. Spec. Publ. No. 6, Amer.Fish. Soc., Washington, D.C. 174 pp.5-13 TABLE 5-1

SUMMARY

OF SAMPLING SCHEDULE FOR SURVEYS OF THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION Year/Gear 1973 Seine JAN FEB MAR APR MAY JUN JUL AUG SEP OCT NOV DEC 1974 Seine 1975 Seine 1,4 1,4 1,4 1,4 1,4 1,4 1,4 1,4 1,4 1,4 1,4 1,4 -Un I 1976 Seine 1977 Electro Seine 1978 Electro Seine 1979 Electro Seine 1980 Electro Seine 1981 Electro Seine 1982 Electro Seine 1985 Electro Seine-1,10,11,4-1,10,4-1,10,11,4-1,10,4-1,10,11,4-1,10,11,4-1,10,4-1,10,11,4-1,10,4-1,10,11,4 I 1,11 1,10,4 1,10,4 1 1,10,11,4 1 1,10,4 1 1,10,11,4 1 1,10,11,4 1 1,10,4-1,10,11,4 1 1,10,4 1 1,10,11,4 1 1,10,4 1 1,10,11,4-1,10,11,4-1,10,4-1,10,11,4-1,10,4-1,10,11,4-1,10,4-1,10,11,4-1,10,4-1,10,11,4 1 1-1,10,4 1-1,10,4 1,11 1,10,4 1,10,11,4 1,10,4 1,10,11,4 1,10,11,4 1,10,4 1,10,11,4 1,10,4 1,10,11,4 1,10,4 1 1,10,4-1 1,10,11,4 1 1,10,4 1-1,10,11,4 1 1,10,11,4 1 1 1 1 1 1 1 1 1 1 1 1 1 1,10,11,4 1,10,4 1,10,4 1,10,4 1 1 1 1 -1 1 1 1 1,10,4 1,10,4 -1,10,4 -1,10,4 1,10,11,4

--1,10,11 1,10,4 -1 10,11,4-1,10,4-1,10,11,4-1,10,4-1,10,11,4 10,4 1,10,4 1986 Electro Seine 1987 Electro Seine Note: Location-1,10,4 1,10,4-1,10,11,4 1,10,11,4 1,10,4 1,10,4 1,10,11 1,10,11,4-1,10,4-1,10,11,4-1,10,4-1,10,11,4 1,10,4 1,10,11,4 1,10,4 1,10,11,4 1,10,4 1,10,11,4 1 was in John Redmond Reservoir prior to 1976.

TABLE 5-2 CHECKLIST OF FISHES COLLECTED FROM THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS Ln!I Hn Scientific Name Lepisosteidee (gars)Lepisosteus platostomus Lepisosteus osseus Cluepidae (herrings)

Dorosoma cepedianum Cyprinidae (carps and minnows)Campostoma anomalum Cyprinus carpio Carassius auratus Hybopsis x punctata Notemigonus crysoleucas Notropis buchanani Notropis luetrensis Notropis rubellus Notropis stramineus Notropis umbratilis Phenacobius mirabilis Pimephales notatus Pimephales promelas Pimephales tenellus Pimephales vigilax Catostomidae (suckers)Carpiodes carpio Carpiodes cyprinus Ictiobus bubalus Ictiobus cyprinellas Ictiobus niger Moxostoma erythrurum Moxostoma macrolepidotum Cycleptus elongatus Ictalaridae (freshwater catfishes)

Ictalurus melas Ictalurus natalis Ictalurus

'2ttus Pylodictis olHvaris Noturus flavus Noturus placidus Noturus nocturnus Cyprinodontidae (topminnows)

Fundulus notatus Poecilliidae (livebearers)

Gambusia affinis Common Name Shortnose gar Longnose gar Gizzard shad x K x x x x x x K x x x X K K x X x x x K X K x x X K K K x x X Central stoneroller Common carp Goldfish Gravel chub Golden shiner Ghost shiner Red shiner Rosyface shiner Sand shiner Redfin shiner Suckermouth minnow Bluntnose minnow Fathead minnow Slim minnow Bullhead minnow River carpsucker Quillback Smallmouth buffalo Bigmouth buffalo Black buffalo Golden redhorse Shorthead redhorse Blue sucker Black bullhead Yellow bullhead Channel catfish Flathead catfish Stonecat Neosho madtom Freckled madtom Blackstripe topminnow X X X K X X X X K x X X X K K X x x x X K K X K K X X X X X X K X X X X X X X x X X X X X X X X X X X X X X X X X X X X K K x K K K x K K K x K K K x K K K K x K K K K K K K K K x K K K K K K K K K K x K x x x x K x K K K K K x x x x x x x x x x x x 1973 1974 1975 1976 1977 1978 1979 1980 1981 1982 1985 1986 1987 K x K K K K x x x x x K x x x x x x x x x x x x K x x x K x X X X X X X x X x X X X X K K x K x K x K K X x K K K K K x x x X X x K x x x x x x x K x x x x x x x x x x x x x K K K x K x K K K K K K x x K K K K x K X X X X X X Mosquitofish X K K K K K X K K K K X TABLE 5-2 (Cont.)Scientific Name Atherinidae (silversides)

Labidesthes sicculus Percichthyidae (temperate basses)Morone chrysops Morone saxatilis x N. chrysops Centrarchidae (sunfishes)

Lepomis cyanellus Lepomis humilis Lepomis macrochirus Lepm5 megalotis Lepomis macrochirus x L. megalotis Micropterus punctulatus Micropterus salmoides Pomoxis annularis Percidae (perches)Etheostoma chlorosomum Etheostoma spectabile Percina phoxocephala Percina caprodes Stizostedion vitreum Sciaenidae (drums)Aplodinotus grunniens No. of Species Accumulated No. of Species Common Name Brook silversides 1973 1974 1975 1976 1977 1978 1979 1980 1981 1982 1985 1986 1987 T X X x X X X X X X White bass Wiper K K x x K K K x K K K K K K x Green sunfish Orangespotted sunfish Bluegill Longear sunfish Hybrid sunfish Spotted bass Largemouth bass White crappie Bluntnose darter Orangethroat darter Slenderhead darter Logperch Walleye x x x K K x x x K K K K K K x x x x x x K K K x K K K K K K x K K K K K X K K K x x x K x K K K K K x K K K K x x x K K K x K x K K x x x x K K x K x K K K K K K K K K K X K K K K K K K K X X X K K X K x K K X K K K K I, 0H Freshwater drum X X X X X X X K X K x K X 30 31 32 37 39 36 39 41 31 29 34 30 39 40 44 46 46 47 50 50 50 51 36 31 52 52 Note: Nomenclature follows Robins 1980.

I..~3 TABLE 5-3 RELATIVE ABUNDANCE OF THIRTEEN PREDOMINANT FISHES IN COMBINED ELECTROFISHING AND SEINING CATCHES FROM THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS Species-Red shiner Gizzard shad Ghost shiner River carpsucker Freshwater drum Carp Channel catfish White crappie Smallmouth buffalo Bigmouth buffalo White bass Mosquitofish Bullhead minnow 1977 1978 1979 1980 1981 1982 1985 1986 1987 31.9 16.2 13.4 6.5 6.2 3.8 3.4 3.0 2.6 2.4 1.6 1.6 0.4 40.0 29.2 4.5 2.5 3.7 2.2 2.4 1.5 1.9 0.5 1.1 1.2 2.9 51.4 8.5 5.2 6.6 4.0 3.0 2.4 5.3 1.4 0.7 2.5<0.1 3.8 77.7 2.6 0.4 3.5 1.2 0.9 0.7 0.7 1.3 0.2 0.5 0.1 4.8 48.3 27.9 4.3 3.2 1.8 1.1 1.4 2.2 1.4 0.3 1.7 0.5 1.6 9.9 14.0 5.9 6.5 7.6 3.7 5.5 6.0 7.4 2.6 10.0 3.9 0.0 51.9 15.4 25.9 0.2 0.7 0.3 0.5 0.6 0.3<0.1 0.0 0.3 0.6 39.8 20.9 22.3 2.1 1.5 1.8 1.7 2.2 0.8 0.3 0.7 0.2 0.3 30.0 16.7 24.5 2.1 1.0 0.9 0.5 1.7 1.3 0.4 1.0 13.1 0.5 Total Catch 3,095 5,950 4,945 4,903 2,914 800 7,579 2,785 2,429 Note: Combined data from Locations 1, 10 and 4, except in 1982 when only Location 1 was surveyed.5-17 TABLE 5-4 NUMBER AND RELATIVE ABUNDANCE OF FISH COLLECTED BY ELECTROFISHING IN THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1977-1982 AND 1985-1987 Species 1977 1978 1979 1980 1981 1982 No. % No. % No. % No. % No. % Noe. %1985 1986 1987 No. % No. % No. %Longnose gar Shortnose gar Gizzard shad Goldfish Common carp Golden shiner Ghost shiner Red shiner Sand shiner Suckermouth minnow Fathead minnow Slim minnow Bullhead minnow Notropis spp.River carpsucker Quillback Blue sucker Smallmouth buffalo Bigmouth buffalo Black buffalo Golden redhorse Shorthead redhorse Black bullhead Channel catfish Flathead catfish Mosquitofish Brook silverside White bass Wiper Green sunfish Orangespotted sunfi Bluegill Longear sunfish Bluegill X Longear Spotted bass Largemouth bass White crappie Walleye Freshwater drum No. Species Total No. Fish Units of Effort Catch Per Unit of Effort 45 11 19 3 7 9 1 1 4 2 Lsh 1 sunfish 8 19 2 1,46 20.73.6 0.4 7 0.5 9 0.6 7 1.3 8 0.7 0 0.0 6 2.3 4 0.7 2 0.1 3 0.2 9 0.6 0 0.0 0 0.0 2 0.4 7 2.6 8 1.4 7 31.1 563 36.7 271 18.5 62 11.4 720 64.4 101 21.2 94 35.5 250 42.6----1 0.1 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 6 7.9 101 6.6 149 10.2 43 7.9 33 3.0 29 6.1 21 7.9 48 8.2------------1 0.4 0 0.0 1 0.1 0 0.0 0 0.0 1 0.2 0 0.0 0 0.0 0 0.0 0 0.0 4 0.3 2 0.1 7 0.5 1 0.2 1 0.1 4 2.6 0 0.0 3 0.5 1 0.1 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0--------------2 0.3... .. .... .....2 0 .3... .. .... .....1 0 .2 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 1 0.4 0 0.0 9 13.5 139 9.1 323 22.1 152 27.8 91 8.1 51 10.7 18 6.8 46 7.8 0 0.0 0 0.0 0 0.0 1 0.2 0 0.0 0 0.0 1 0.4 3 0.5 3 2.2 38 2.5 21 1.4 7 1.3 10 0.9 8 1.7 1 0.4 8 1.4'9 5.4 63 4.1 58 4.0 46 8.4 42 3 .8 58 12.2 24 9.1 22 3.8 73 5.0 28 1.8 36 2.5 8 1.5 8 0.7 21 4.4 6 2.3 8 1.4 1 0.1 5 0.3 9 0.6 2 0.4 0 0.0 0 0.0 1 0.4 0 0.0 3 0.2 1 0.1 1 0.1 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 1 0.1 3 0.2 1 0.1 2 0.4 7 0.6 6 1.3 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 3 1.1 0 0.0 3 6.3 78 5.1 93 6.4 35 6.4 37 3.3 40 8.4 5 1.9 40 6.8 4 1.0 17 1.1 11 0.8 42 7.7 18 1.6 22 4.6 1 0.4 34 5.8 0 0.0 1 0.1 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 0 0.0 5 1.0 0 0.0 0 0.0 2 0.4 15 1.3 0 0.0 0 0.0 1 0.2 8 3.3 54 3.5 120 8.2 15 2.7 35 3.1 31 6.5 9 3.4 10 1.7------------3 1.1 1 0.2 6 1.8 153 10.0 52 3.6 15 2.7 3 0.3 2 0.4 5 1.9 22 3.8 2 0.1 3 0.2 1 0.1 0 0.0 2 0.2 2 0.4 3 1.1 5 0.8 2 0.1 0 0.0 0 0.0 0 0.0 2 0.2 1 0.2 2 0.8 1 0.2 1 0.7 9 0.6 4 0.3 5 0.9 3 0.3 4 0.8 4 1.5 5 0.8--------------1 0.2 8 0.5 6 0.4 9 0.6 5 0.9 0 0.0 0 0.0 2 0.8 4 0.7 1 0.1 1 0.1 2 0.1 1 0.2 2 0.2 1 0.2 0 0.0 1 0.2 3 5.7 31 2.0 75 5.1 30 5.5 25 2.2 34 7.1 2 0.8 16 2.7 0 0.0 5 0.3 8 0.5 4 0.7 4 0.4 3 0.6 0 0.0 0 0.0 0 12.9 221 14.4 192 13.1 60 11.0 52 4.6 57 11.9 45 17.0 41 7.0 5 2 276 0 21 0 1 1 0 0 1 0 0 0 31 0 2 31 10 0 0 1 2 6 23 0 0 6 0 3 1 0 7 0 3 0 6 0 23 1 .1 0.4 59.7 0.0 4.5 0.0 0.2 0.2 0.0 0.0 0.2 0.0 0.0 0.0 6.7 0.0 0.4 6.7 2.2 0.0 0.0 0.2 0.4 1.3 5.0 0.0 0.0 1.3 0.0 0.6 0.2 0,0 1.5 0.0 0.6 0.0 1.3 0.0 5.0 6 9 0 4 24 1,532 17.0 90.1 24 1,462 18.0 81.2 23 546 14.0 39.0 21 1,118 18.0 62.1 20 477 9.0 53.0 24 265 16.5 16.1 25 587 17.9 32.8 22 462 12.0 38.5 Note: 1977-81 and 1985-87 data include fish collected Location 1.from Locations 1, 4, and 10; 1982 data only from TABLE 5-5

SUMMARY

OF PREOPERATIONAL (1977-82)

AND OPERATIONAL (1985-87)CATCH DATA FOR PREDOMINANT FISHES COLLECTED BY ELECTROFISHING IN THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS Pre-Operational Operational Catches Catches (1977-82)

(1985-87)Species No. _ CPE No. % CPE Gizzard shad 2,714 32.9 20.3 620 47.2 13.4 Carp 471 7.1 4.4 90 6.8 1.9 River carpuscker 955 14.5 8.9 95 7.2 2.0 Smallmouth buffalo 346 5.2 3.2 77 5.9 1.6 Bigmouth buffalo 174 2.6 1.6 24 1.8 0.5 Channel catfish 376 5.7 3.5 51 3.9 1.1 Flathead catfish 124 1.9 1.2 58 4.4 1.2 White bass 303 4.6 2.8 22 1.9 0.5 White crappie 278 4.2 2.6 24 1.8 0.5 Freshwater drum 772 11.7 7.2 109 8.3 2.3 5-19 TABLE 5-6 AVERAGE ELECTROFISHING CPE FOR PREDOMINANT FISHES IN THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1977-1982 AND 1985-1987 Year Species 1977 1978 1979 1980 1981 1982 1985 1986 1987 Mean Location 1 Gizzard shad 53.9 67.1 28.5 5.3 66.6 11.2 15.6 8.8 65.0 35.8 River carpsucker 18.1 13.2 35.8 20.2 6.9 5.7 1.8 3.1 4.8 12.2 Freshwater drum 6.9 9.6 9.8 3.5 1.9 6.3 5.8 2.5 1.8 5.3 Channel catfish 8.1 5.3 6.5 5.2 3.3 4.4 0.4 5.4 0.5 4.3 Smallmouth buffalo 5.4 3.7 3.8 3.3 2.3 6.4 1.8 1.4 3.2 3.5 White bass 5.6 7.3 14.2 2.3 3.3 3.4 1.8 1.5 1.5 4.5 White crappie 10.0 4.0 8.1 4.7 2.5 3.8 0.4 2.2 0.8 4.1 Carp 1.8 6.4 11.5 5.5 2.0 3.2 1.8 1.2 3.2 4.1 Bigmouth buffalo 9.1 3.1 2.0 1.3 0.7 2.3 0.9 1.0 2.0 2.5 Flathead catfish 1.4 1.7 1.0 3.5 0.6 2.4 0.2 2.2 1.5 1.6 Total CPE 120.3 121.4 121.2 54.8 90.1 49.1 30.5 29.3 84.3 77.9 Location 10 Gizzard shad 1.1 10.2 6.4 0.8 5.3 -3.8 32.0 2.0 7.7 River carpsucker 7.7 3.8 3.4 3.8 3.0 -1.2 1.8 0.8 3.2 Freshwater drum 14.0 17.4 15.8 8.5 5.8 -1.3 2.5 2.2 8.4 Channel catfish 4.0 6.2 4.4 0.8 0.3 -0.3 1.0 0.8 2.2 Smallmouth buffalo 4.3 5.0 4.0 5.2 2.0 -1.3 1.0 3.0 3.2 White bass 0.2 0.2 0.6 0.0 2.0 -0.2 0.0 0.0 0.4 White crappie 0.3 0.2 1.6 0.5 0.0 -0.0 0.2 0.0 0.4 Carp 12.3 8.8 3.8 2.2 1.5 -1.3 4.8 1.5 4.5 Bigmouth buffalo 0.0 1.2 3.0 0.0 0.3 -0.0 0.2 0.5 0.6 Flathead catfish 0.2 0.4 0.4 2.5 0.3 -0.0 0.7 0.5 0.6 Total CPE 44.1 53.4 43.4 24.3 20.5 -9.4 44.2 11.3 31.3 Location 4 Gizzard shad 3.2 8.4 2.2 6.8 8.3 -0.2 1.0 2.0 4.0 River carpsucker 1.7 5.4 4.0 4.0 2.5 -0.5 2.8 2.2 2.9 Freshwater drum 8.5 13.4 7.0 1.2 2.5 -1.8 1.8 1.8 4.8 Channel catfish 0.7 2.0 3.8 0.2 0.8 -0.2 0.3 0.2 1.0 Smallmouth buffalo 1.7 2.4 1.6 1.2 2.8 -1.3 1.3 1.5 1.7 White bass 0.3 0.4 0.6 0.2 0.3 -0.0 0.2 0.0 0.2 White crappie 0.3 0.4 0.4 0.0 0.0 -0.0 0.3 0.8 0.3 Carp 4.7 2.4 7.6 0.2 1.8 -0.8 2.0 0.5 2.5 Bigmouth buffalo 0.2 0.0 1.0 0.0 0.0 -0.3 0.2 0.0 0.2 Flathead catfish 0.3 0.6 0.2 2.8 2.8 -0.0 2.8 3.8 1.7 Total CPE 21.6 35.4 28.4 17.8 21.8 -5.1 12.7 12.8 19.4 Notes: 1. CPE = number of fish caught per 30 min. of effort.2. Dash (-) indicates location not sampled.5-20 TABLE 5-7 NUMBER OF SELECTED FISHES COLLECTED BY SEINING IN THE NEOSHO RIVER NEAR THE WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1973 THROUGH 1981 AND 1985 THROUGH 1987 Species Year 1973 1974 1975 1976 1977 1978 1979 1980 1981 1985 1986 1987 Gizzard shad Ghost shiner Red shiner Bluntnose minnow Bullhead minnow Slim minnow Neosho madtom mosquitofish Total fish (All species)No. collections No. fish per collection 0 35 42 0 92 0 0 21 910 2 2 7 3 100 1,558 3 14 64 1,669 606 2,928 43 238 32 12 54 5,944 41 412 981 31 12 6 19 49 1,626 1,174 270 2,381 11 174 0 46 69 4,418 149 264 2,536 29 188 22 12 2 3,491 66 21 3,808 3 236 6 6 4 4,357 94 125 1,405 0 47 13 34 15 1,829 1,072 1,962 3,934 0 42 113 8 24 7,314 333 621 1,153 2 6 15 19 6 2,254 130 594 728 13 13 4 1 317 1,956 0 0 0 0 24 108 207 990 1,920 8 8 8 26 124 240 26 229 26 28 63 158 25 140 14 16 311 114 17 430 18 124 11 179 U' TABLE 5-8

SUMMARY

OF PREOPERATIONAL (1976-82)

AND OPERATIONAL (1985-87)

CATCH DATA FOR PREDOMINANT FISHES COLLECTED BY SEINING IN THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS Preoperational catches Average catch No. % per collection Operational catches Average catch No. % per collection Species Gizzard shad Ghost shiner Red shiner Bullhead minnow Mosquitofish Game fish Other Total 3,088 1,711 12,836 766 219 729 819 20,168 15.3 8.5 63.6 3.8 1.1 3.6 4.1 29.7 16.5 123.4 7.4 2.1 7.0 7.9 193.9 1,535 13.4 3,177 27.6 5,768 50.2 61 0.5 347 3.0 237 2.1 33.4 69.1 125.4 1.3 7.5 5.2 7.9 249.7 362 11,487 3.2 5-22 TABLE 5-9 NUMBER OF PREDOMINANT FISHES COLLECTED BY SEINING AT THREE LOCATIONS ON THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION 1976-82. AND 1985-87 Species Location 1 Gizzard shad Ghost shiner Red shiner Bullhead minnow Mosquito fish Game fish Other Total Location 10 Gizzard shad Ghost shiner Red shiner Bullhead minnow Mosquito fish Game fish other Total Location 4 Gizzard shad Ghost shiner Red shiner Bullhead minnow Mosquito fish Game fish Other Total 1976 1977 1978 1979 1980 1981 1982 1985 1986 1987 Total No. Per No. Sampling Date% of Total 1,664 422 49 0 5 194 39 2,373 4 87 433 8 10 13 43 598 1 77 1,222 106 34 8 65 1,513 37 195 130 0 0 13 30 405 4 71 276 2 12 8 25 398 0 143 554 8 37 3 25 770 1,054 205 438 0 42 74 58 1,871 2 19 787 66 13 13 61 961 2 44 1,131 108 14 14 54 1,367 148 165 153 0 0 179 38 683 1 7 1,068 26 1 19 25 1,147 0 83 1,302 159 1 13 51 1,609 29 18 1,184 17 4 17 81 1,350 0 3 1,987 215 0 4 40 2,249 37 0 637 4 0 22 55 755 88 13 146 0 3 46 16 312 11 47 78 0 31 72 84 323 4 44 308 7 0 8 17 388 2 68 953 40 12 9 12 1,096 1,056 540 535 2 13 45 43 2,234 16 245 1,095 23 6 39 33 1,457 0 1,177 2,304 17 5 12 108 3,623 297 317 59 0 0 49 7 729 4 103 273 1 1 7 33 422 32 201 774 5 5 11 38 1,066 78 30 30 0 6 36 18 198 5 133 464 2 143 4 24 775 47 431 234 11 168 34 58 983 4,462 1,952 2,802 19 104 725 414 10,478 40 712 6,691 350 186 115 301 8,395 121 2,224 9,111 458 276 126 466 12,782 72.0 31.5 45.2 0.3 1.7 11.7 6.7 169.0 0.9 15.8 148.7 7.8 4.1 2.6 6.7 186.6 2.8 51.7 211.9 10.7 6.4 2.9 10.8 297.3 42.6 18.6 26.7 0.2 1.0 6.9 4.0 0.5 8.5 79.7 4.2 2.2 1.4 3.6 0.9 17.4 71.3 3.4 2.2 1.0 3.6 Ln IJ APPENDIX A SUPPLEMENTAL WATER QUALITY FIGURES Abbreviated Title Temporal variations in total suspended solids (TSS)Figure A-I A-2 A-3 A-4 A-5 A-6 A-7 A-8 A-9 A-10 A-il A-12 A-13 A-14 A-15 A-16 Temporal Temporal Temporal Temporal Temporal Temporal Temporal Temporal Temporal Temporal Temporal Temporal Temporal Temporal Temporal variations variations variations variations variations variations variations variations variations variations variations variations variations variations variations in in in in in in in in in in in in in in in calcium concentrations magnesium concentrations soluble orthophosphate total iron concentrations total alkalinity nitrate concentrations total dissolved solids (TDS)sulfate concentrations total organic nitrogen (TON)nickel concentrations ammonia concentrations chemical oxygen demand (COD)biochemical oxygen demand (BOD)copper concentrations chromium concentrations Page A-i A-2 A-3 A-4 A-5 A-6 A-7 A-8 A-9 A-10 A-il A-12 A-13 A-14 A-15 A-16 Total Suspended Solids (TSS)NEOSHO RIVER 500 450 400 350 300 250 200 E C a 150 100 50 0 74 76 78 80 82 84 86 88 Year Total Suspended Solids (TSS)WCGS Cooling Lake E E C 350 300 250 200 150 100 50 0 81 83 85 87 Year Figure A-1.Temporal variations in total suspended solids (TSS) for the Neosho River and WCGS Cooling Lake. 1974-1987.

A-1 Calcium NEOSHO RIVER 130 120 110 100 C=E 90 80 70 60 50 40 30 20 74 76 78 80 82 84 86 88 Year Calcium WCGS Cooling Lake 120 110 -100-90 70 50 -t E C 0=0 40-30 10-0-81 II I I £83 85 87 Year Figure A-2.Temporal variations in calclurn concentrations for the Neosho River and WCGS Cooling Lake. 1974-1987.

A ~

Magnesium NEOSHO RIvER 40 35 30 25 20 V E oC 0 15 10 5 74 76 78 80 82 84 86 88 Year Magnesium WCGS Cooling Lake 50 40 -0 E C 30 10-0.4 a'83 85 87 Year Figure A-3.Temporal variations in magnesium concentrations for the Neosho River and WCGS Cooling Lake. 1974-1987.

A-3 Orthophospha te NEOSHO RIVER E c a S 0.26 0.24 0.22 0.2 0.18 0.16 0.14 0.12 0.1 0.08 0.06 0.04 0.02 0 74 76 78 80 82 84 86 88 Year Orthophosphate WCGS Cooling Lake L E c 0.20 0.19 0.18 0.17 0.16 0.15 0.14 0.13 0.12 0.11 0.10 0.09 0.08 0.07 0.06 0.05 0.04 0.03 0.02 0.01 0.00 81 83 85 87 Year Figure A-4.Temporal variations in soluble orthophophate for the Neosho River and WCGS Cooling Lake, 1974-1987.

A-4 Total Iron NEOSHO RIVER 7 6 5 4 3 2 I C, E c a 0;'p U 0 74 76 78 80 82 84 86 88 Year Total Iron WCGS Cooling Lake 10 9 8 7 0 C)E C a 0 6 5 4 3 2 1 81 83 85 87 Year Figure A-5.Temporal variations in total iron concentrations for the Neosho River and WCGS Cooling Lake. 1974-1987.

A-5 Total Alkalinity NEOSHO RIVER E C 380 360 340 -320 300 280 260 240 220 -200 14 160 ~140 120 100 s0o-L~]-Till~t iI U 1~1 60 74 76 78 80 82 84 86 88 Year Total Alkalinity WCGS Cooling Lake 1 E c a S 2I 300 280 260 240 220 200 180 160 140 120 100 80 60 40 20 0 81 83 85 87 Year Figure A-6. Temporal variations in total alkalinity for the Neosho River and WCGS Cooling Lake. 1974-1987.

A-6 Nitrate NEOSHO RIVER E 0 2.1 2 1.9 1.8 1.7 1.6 1.5 1.4 1.3 1.2 1.1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 74 76 78 80 82 84 86 88 Year Nitrate WCGS Cooling Lake S E C 10 9 8 7 6 5 4 3 2 0 0 81 83 85 87 Year Figure A-7.Temlporal variations in nitrate concentrations for the Neosho River and WCGS Cooling Lake. 1974-1987.

A-7 Total Dissolved Solids NEOSHO RIVER 600 550 500 450= 400 0 S 350 ,300 -I3 250 0 200 -150 I , 74 76 78 80 82 84 86 88 Year Total Dissolved Solids (TDS)WCGS Cooling Lake 500 -400 300 E c a 200.100 0 Ii 81 83 85 87 Year Figure A-B. Temporal variations in total dissolved solids (TDS) for the Neosho River and WCGS Cooling Lake, 1974-119B7.

A-8 Sulfates NEOSHO RIVER C E C 0 140 130 120 110 100 90 80 70 60 50 40 30 20 10 74 76 78 80 82 84 86 88 Year Sulfates WCGS Cooling Lake 120 110 100 -9800 I E 0 CE 70 50 30 10 -0 81 I I I 1 I 83 85 87 Year Figure A-9.Temporal variations in sulfate concentrations for the Neosho River and WCGS Cooling Lake. 1974-1987.

A-9 Total Organic Nitrogen NEOSHO RIVER E C 2.1 2 1.9 1.8 1.7 1.6 1.5 1.4 1.3 1.2 1.1 1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 74 76 78 80 82 84 86 88 Year Total Organic Nitrogen WCGS Cooling Lake 5.0 4.0 E C 0 3.0 2.0 1.0 0.0 81 83 85 87 Year Figure A-IO.Temporal variations in total organic nitrogen (TON) for the Neosho River and WCGS Cooling Lake. 1974-1987.

^-1fl Nickel NEOSHO RIVER 80 70 60 50 40 30 C a 20 10 0 50i 74 75 78 80 82 84 86 88 Year Nickel WCGS Cooling Lake C oa c 30 10-0 81 83 85 87 Year Figure A-1 I.Temporal variations in nickel concentrations for the Neosho River and WCGS Cooling Lake. 1983-1987.

A-I1 AMMONIA NEOSHO RIVER 0.5 0.4 C E C 0 0.2 0.1 0.0 1.0.1 0.9 0.8 0.7 4 74 76 78 80 82 84 86 88 Year Ammonia WCGS Cooling Lake E C o C 0.6 -0.5 -0.4 -0.3 -0.2 -0.1 -0.0 81 83 85 87 Year Figure A-12.Temporal variations in armrnonia concentrations for the Neosho River and WCGS Cooling Lake, 1974-1987.

A-1 ')

Chemical Oxygen Demand (COD)NEOSHO RIVER 110 100 90 80 V E 0 70-60 40-30 10 -0 0 I I i I I i I I I I 74 76 78 80 82 84 I I l 86 88 Year Chemical Oxygen Demand (COD)WCGS Cooling Lake 100 904.80 70 -60 E 50 t: 40 30-20 10 0 81 83 85 87 Year Figure A-13.Temporal variations in chemical oxygen demand (COD) for the Neosho River and WCGS Cooling Lake. 1974-1987.

A-13 Biochemical Oxygen Demand (BOD)NEOSHO RIVER R L E£7 5 3 I1-0 I I I I I I I I I I 74 76 78 80 82 84 86 Year Biochemical Oxygen Demand (BOD)WCGS Cooling Lake 8 88 10 E C 0 9-8-7-6-5-4-3-2 1 -0 81 83 85 87 Year Figure A-14.Temporal variations in biochemical oxygen demand (BOD) for the Neosho River and WCGS Cooling Lake. 1974-1987.

A-1 4 Copper NEOSHO RIVER 32 30 28 26 24.22 20 8 18 C 1 n13 2 -10 0 -! I I I I I I I 1 I 74 76 78 80 82 84 86 88 Year Copper WCGS Cooling Lake 50 -40 30o 20 10 I0I I I 81 83 85 87 Year Figure A-15. Temporal variations in copper concentrations for the Neosho River and WCGS Cooling Lake. 1974-1987.

A-15 Chromium NEOSHO RIVER 100 90 80 70 C o 0 60 40-3 47 1 30 10 -120 110 100 90 0o -I I I I I I I I I 1 74 76 78 80 82 84 86 8 Year Chromium WCGS Cooling Lake i8 8 L C'a U 80 70 60 50 40 30 20 10 0 81 83 85 Year 87 Figure A-16.Temporal variations in chromiuru concentrations for the Neosho River and WCGS Cooling Lake. 1983-1987.

A-16 Table B-1 B-2 B-3 B-4 B-5 B-6 B-7 B-8 B-9 B-10 B-11 B-12 B-13 APPENDIX B 1987 DATA TABLES Contents Abbreivated Title Replicate water chemistry data ... 2 March 1987 Replicate water chemistry data ... 28 April 1987 Replicate water chemistry data ... 23 June 1987 Replicate water chemistry data ... 24 August 1987 Water quality data from groundwater samples ... 1987 Phytoplankton and Macroinvertebrate Macroinvertebrate Macroinvertebrate Macroinvertebrate Macroinvertebrate Macroinvertebrate Macroinvertebrate zooplankton production

... 1987 species densities

... 2 March 1987 species densities

... 27 & 28 April 1987 species densities

... 22 June 1987 species densities

... 24 August 1987 family densities

... 2 March 1987 family densities

... 27 & 28 April 1987 family densities

... 22 June 1987 Page B-1 B-2 B-3 B-4 B-5 B-6 B-7 B-9 B-11 B-13 B-15 B-17 B-19 B-20 B-21 B-22 B-25 B-14 Macroinvertebrate family densities

... 24 August 1987 B-15 Physical measurements recorded during fish surveys in the Neosho River ... 1987 B-16 Electroshocking catch data for the Neosho River ... 1987 B-17 Seine data for the Neosho River ... 1987 TABLE B-i REPLICATE WATER CHEMISTRY DATA FOR GENERATING STATION. 2 MARCH 1987 SAMPLES COLLECTED FROM THE NEOSHO RIVER AND THE COOLING LAKE FOR WOLF CREEK Parameter Units IA 1B 10A 10B 4A 48 2A 2B 6A 6B 8A 8B Water Temperature Dissolved Oxygen pH Conductivity Total Alkalinity Turbidity Residue (TDS)(TSS)Biochemical Oxygen Demand Oil and Grease Bacteria, Fecal Coliform Calcium Total Chromium Copper Total Iron Soluble Iron Magnesium Nickel Chemical Oxygen Demand chloride Ammonia-Nitrogen Nitrite-Nitrogen Nitrate-Nitrogen Orthophosphate Sulfate C mg/i units pmhos/cm mg/i NTU mg/l mg/i mg/i mg/l No./100 ml mg/l pg/l pg/l mg/l mg/l mg/l pg/1 mg/l mg/1 mg/l mg/i mg/I mg/1 mg/l 7.0 13.7 8.0 420 310 48 298 76 4.0 19 53.2 0.006 0.045 2.7 0.02 19.2 0.001 20 12 0.24 0.04 0.45 0.02 58-8.0 13.8 13.2 8.1 7.2 420 440 380 310 50 58 258 304 82 100 3.45 1.0 3.1 (3 17 88 56.0 (0.1 0.006 0.025 0.010 0.013 2.4 3.1 0.10 0.6 15.6 15.6 0.017 0.006 (10 30 11 12 0.23 0.32 0.04 0.06 0.44 0.77 0.02 0.06 58 55-8.0 13.4 12.3 7.4 7.1 440 410 300 320 52 88 284 624 86 52 1.34 3.56 (3 (3 94 56 (0.1 61.1 0.010 0.007 0.009 0.010 3.7 3.2 0.21 0.06 15.4 18.6 0.004 0.006 (10 30 10 10 0.30 0.36 0.06 0.06 0.55 0.43 0.06 0.06 56 54-12.5 12.7 12.0 7.2 7.9 410 320 290 250 90 17 628 164 44 4 5.41 2.0<3 (3 38 55 56.2 33.5 0.009 0.006 0.016 0.010 5.2 0.77 0.10 1.60 17.6 12.1 0.011 0.002 (10 20 10 15 0.36 0.12 0.07 0.02 0.46 0.14 0.06 0.02 53 49 7.9 320 243 18 232 4 2.3<3 38 32.7 0.004 0.004 0.73 0.06 12.5<0.001 20 15 0.14 0.02 0.22 0.03 48 10.4 7.9 320 251 5 272 8 2.9 (3 0 36.8 0.003 0.008 0.15<0.02 14.9<0.001<10 16 0.19<0.01 0.17 0.01 50 7.9 320 256 5 146 4 2.6<3 0 40.1 0.004 0.008 0.19 (0.102 15.6 (0.001 10 16 0.16 (0.01 0.21 0.03 50 6.0 13.6 7.9 320 260 11 216 12 2.2 (3 1 38.4 0.003 0.029 0.34 0.02 15.2 (0.001<10 15 0.23<0.01 0.15 0.03 49 7.9 320 230 9 128 12 2.3 4 39.0 0.003 0.018 0.35 0.02 16.2<0.001 10 16 0.24 (0.01 0.13 0.04 49 TABLE B-2 REPLICATE WATER CHEMISTRY DATA FOR GENERATING STATION. 28 APRIL 1987 SAMPLES COLLECTED FROM THE NEOSHO RIVER AND THE COOLING LAKE FOR WOLF CREEK Parameter Units 1A 1B 10A 10B 4A 4B 2A 25 6A 6B SA 8B Water Temperature Dissolved Oxygen pH Conductivity Total Alkalinity Turbidity Residue (TDS)(TSS)Biochemical Oxygen Demand Oil and Grease Bacteria, Fecal Coliform Calcium Total Chromium Copper Total Iron Soluble Iron Magnesium Nickel Chemical Oxygen Demand Chloride tAmmonia-Nitrogen Organic Nitrogen Nitrite-Nitrogen Nitrate-Nitrogen Orthophosphate Sulfate C mg/1 units pmhos/cm mg/1 NTU mg/i mg/l mg/i mg/l No./100 ml mg/l pg/l pg/i mg/l mg/l mg/l Pg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l 20.0 9.5 7.4 370 392 42 306 42 3.0<3 23 50.4 0.006 0.007 3.70 0.06 10.0 0.002 20 10 0.06 0.3 0.07 i .83 0.03 5o 9.6 7.7 380 354 42 326 28 3.3 (3 23 53.7 0.002 0.001 0.45 0.06 5.0 0.001 10 10 0.06 0.4 0.07 0.87 0.03 49 20.5 9.2 8.1 420 385 39 266 76 2.6<3 10 66.0 0.003 0.006 2.14 0.19 11.2 0.001 20 11 0.04 0.4 0.06 0.88 0.03 51 9.3 8.3 420 383 37 224 146 2.1<3 46 62.2 0.003 0.015 1.80 0.06 11.0 0.002 40 11 0.03 0.5 0.06 1.01 0.03 50 20.5 8.1 420 378 37 352 44 3.7 (3 36 63.5 0.005 0.009 3.42 0.06 11.2 0.002 20 11 0.13 0.2 0.05 0.79 0.03 52 9.7 8.1 430 376 37 326 60 3.1<3 10 54.7 0.004 0.005 2.64 0.06 10.8 0.001 10 11 0.04 0.5 0.05 0.99 0.03 52 22.0 8.0 8.0 300 305 2.8 250 1 1.7 (3 0 38.8 0.001 0.006 0.15 0.08 9.0 0.001 30 15 0.11 0.9 (0.01 0.77<0.01 50 8.1 310 296 2.8 224 1 2.3'3 0 37.0 0.002 0.005 0.15 0.15 8.3<0.001 20 15 0.11 0.6 0.01 0.22<0.01 50 20.0 8.6 8.0 300 291 2.5 136 8 1.6 (3 0 42.6 0.002 0.005 0.15 0.06 9.4 (0.001 30 15 0.09 0.7 0.01 0.03<0.01 50 8.2 320 294 2.0 198 16 1.5 (3 0 38.0 0.002 0.004 0.15 0.08 9.2 (0.001 40 15 0.09 0.4 0.01 0.02 (0.01 51 18.5 8.6 8.0 380 292 2.5 112 16 1.4 (3 0 40.0 0.002 0.003 0.26 0.06 9.1 0.007 30 15 0.10 0.6 (0.01 0.08 (0.01 49 8.2 325 295 2.2 214 2 2.2<3 0 40.3 0.002 0.009 0.26 0.10 9.2 0.002 40 is 0.10 0.9 (0.01 0.03 (0.01 50 TABLE B-3 REPLICATE WATER CHEMISTRY DATA FOR GENERATING STATION, 23 JUNE 1987 SAMPLES COLLECTED FROM THE NEOSHO RIVER AND THE COOLING LAKE FOR WOLF CREEK Parameter Water Temperature Dissolved Oxygen PH Conductivity Total Alkalinity Turbidity Residue (TDS)(TSS)Biochemical Oxygen Demand Oil and Grease Bacteria, Fecal Coliform Calcium Total Chromium Copper Total Iron Soluble Iron Magnesium Nickel Chemical Oxygen Demand Chloride Ammonia-Nitrogen Organic Nitrogen Nitrite-Nitrogen Nitrate-Nitrogen Orthophosphate Sulfate Units 1A IB 1OA 10B 4A 4B 2A 2B 6A 6B 8A 8B C mg/l units pmhos/cm mg/i NTU mg/1 Ug/l mg/i mg/l No./100 ml mg/i mg/i pg/l mg/l mg/l mg/l pg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l mg/l 30.5 7.9 8.3 450 170 38 204 5o 3.2 (3 124 52.4 0.018 0.012 2.27 (0.02 12.5 0.006<10 10 0.06 1.5 0.03 0.82 0.02 66 7.6 8.3 460 174 33 180 50 3.1<3 37 46.7 0.006 0.008 1.90 (0.02 13.6 0.005<10 10 0.10 1.4 0.03 0.82 0.02 66 30.0 7.5 8.1 460 174 47 216 64 1.6 (3 31 52.8 0.008 0.004 3.02<0.02 15.4 0.006<10 10 (0.03 1.2 (0.O0 1.14 0.05 66 7.4 8.3 440 176 30 238 66 1.7 (3 35 53.0 0.007 0.006 2.04<0.02 15.3 0.005 (10 10 0.06 1.0 (0.01 1.04 0.03 66 29.0 7.8 7.8 470 181 34 194 70 1.9 (3 32 51.6 0.012 0.013 2.18 (0.02 13.5 0.005<10 11 (0.03 1.2 (0.01 1.04 0.03 66 7.7 7.9 470 178 35 186 66 1.5 (3 37 56.7 0.010 0.006 2.48<0.02 15.5 0.011 (10 10 0.06 1.0<0.01 1.08 0.05 66 33.0 7.0 8.4 400 142 5 186 2 2.6 1 40.3 0.003 0.005 0.11 (0.02 13.4 0.005 (10 15 (0.03 0.7<0.01 0.03 0.02 62 8.5 400 142 3 164 4 1.5 (3 1 40.6 0.002 0.001 0.10 (0.02 13.2 0.002 (10 15 (0.03 0.9 (0.01 0.12 (0.01 62 27.5 8.2 8.0 400 138 2 160 10 1.1 (3 0 42.2 0.003 0.007 0.10 (0.02 13.8 0.003 (10 14 (0.03 0.5 (0.01<0.01 (0.01 63 8.1 400 139 3 168 4 1.0<3 1 40.0 0.003 0.003 0.04 (0.02 13.2 0.002<10 15 (0.03 0.8 (0.01 0.02 (0.01 62 27.5 8.2 8.6 400 140 3 148 14 1.6 (3 0 40.5 0.003 0.003 0.12 (0.02 13.7 0.002 (10 14 (0.03 0.7 (0.01 0.04 (0.01 62 8.5 400 142 5 176 2 1.2 (3 0 37.3 0.003 0.002 0.12 (0.02 13.0 0.002<10 13 (0.03 0.7<0.01 0.06<0.01 62

-P -r~-~TABLE B-4 REPLICATE WATER CHEMISTRY DATA FOR GENERATING STATION, 24 AUGUST 1987 SAMPLES COLLECTED FROM THE NEOSHO RIVER AND THE COOLING LAKE FOR WOLF CREEK Parameter Units 1A lB 10A 10B 4A 4B 2A 2B 6A 6B BA 8B Water Temperature Dissolved Oxygen pH Conductivity Total Alkalinity Turbidity Residue (TDS)(TSS)Biochemical Oxygen Demand Oil and Grease Bacteria, Fecal Coliform Calcium Total Chromium Copper Total Iron Soluble Iron Magnesium Nickel Chemical Oxygen Demand Chloride tAmmonia-Nitrogen I Nitrite-Nitrogen Nitrate-Nitrogen Orthophosphate Sulfate C mg/l units pmhos/cm mg/l KTU mg/l mg/i ag/i mg/i No./100 ml mg/l pg/l pg/l mg/l mg/i mg/i Pg/l mg/l mg/l mg/l mg/i mg/l mg/l mg/l 8.8 7.6 380 125 33 280 40 1.0<3 102 43.9 0.005 0.005 2.6 0.03 9.45 0.006 30 14 0.04 0.10 0.42 0.06 37-----26.0 9.0 8.9 8.8 7.9 7.8 7.4 7.3 7.5 7.4 7.4 7.4 7.9 380 375 380 380 375 400 136 126 130 129 132 120 33 33 30 35 38 4.0 272 288 266 274 270 244 50 400 60 52 50 6 1.0 0.6 0.8 0.8 0.8 1.1 (3 (3 <3 <3 <3 (3 86 54 62 22 34 1 42.6 36.1 36.4 44.3 41.4 29.9 0.005 0.008 0.008 0.48 0.020 0.006 0.003 0.007 0.007 0.036 0.012 0.051 3.7 3.4 2.9 5.9 5.8 2.6 0.02 0.03 0.02 0.02 0.02 <0.03 11.8 10.3 10.5 11.0 9.12 10.8 0.003 0.011 0.009 0.014 0.005 0.029 30 30 60 30 30 40 14 14 14 15 13 19 0.05 0.04 0.04 0.04 0.05 0.06 0.10 0.06 0.06 0.06 0.06 0.01 0.43 0.59 0.54 0.55 0.55 0.02 0.07 (0.01 <0.01 0.07 0.07 <0.01 36 33 35 34 33 44 7.4 400 125 3.5 244 6 0.4 (3 2 31.2 0.006 0.018 2.5 0.02 5.84 0.013 70 19 0.06 0.01 0.02<0.01 45 26.0 7.7 7.6 360 115 3.7 238 2 0.2 (3 0 36.1<0.003<0.003 0.21 0.02 12.4<0.001 70 19 0.06 0.01 0.03<0.01 45 7.7 360 115 1.2 246 8 (3 0 35.7<0.003<0.003 0.13 0.02 11.6 0.001 30 19 0.04 0.01 0.02<0.01 45 27.0 7.6 7.5 360 115 6.3 256 6 0.4<3 0 38.3 0.003 0.003 0.46 0.02 12.5<0.001 70 19 0.09 (0.01 0.01 (0.01 45 7.5 360 118 5.0 244 4 0.3<3 2 36.4 0.004<0.003 0.36 0.01 12.6<0.001 60 18 0.05<0.01<0.01<0.01 45 TABLE B-5 WATER QUALITY DATA FROM GROUNDWATER SAMPLES COLLECTED IN THE VICINITY OF WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1987 MARCH APRIL Parameter Units B12 C20 C49 D65 B]2 C20 C49 D65 Temperature C _(a) ---16.5 14.5 19.0 14.5 pH units 7.2 7.3 7.0 7.4 8.2 7.3 7.3 6.9 Alkalinity mg/l 310 881 523 515 1,057 592 740 296 Conductivity Umhos/cm 3,400 1,000 1,100 1,100 1,100 1,100 1,000 2,600 Hardness -Total mg/l 929 613 532 608 528 659 490 1,010 Residue (TDS) mg/i 2,490 836 1,090 1,922 802 1,040 610 2,454 Calcium mg/i 227 213 135 209 155 240 130 317 Chloride mg/i 800 360 56 310 67 200 55 400 Magnesium mg/i 88 19.8 47.4 21.0 34 14.5 40.1 53 Sulfate mg/i 70 58 20 58 19 50 90 42 Nitrate (as N) mg/i 290 61 7.4 54 0.52 42.3 5.11 220 Iron -Total mg/i 10.5 1.82 0.06 5.34 3.76 1.76 0.13 0.54 Iron -Soluble mg/i 0.17 0.04 0.10 0.04 0.15 0.11 0.21 0.10 AUGUST B12 C20 C49 D65 Temperature C ----pH units 7.0 7.4 7.0 6.9 Alkalinity mg/i 200 265 360 148 Conductivity limhos/cm 2,00 1,220 1,000 2,400 Hardness -Total mg/i 572 608 408 996 Residue (TDS) mg/l 1,144 1,080 824 2,310 Calcium mg/i 205 214 111 286 Chloride mg/l 160 170 46 420 Magnesium mg/i 15.6 17.9 31.8 68.4 Sulfate mg/1 54 52 29 77 Nitrate (as N) mg/i 52 47 0.84 240 Iron -Total mg/1 2.1 3.7 <.01 21 Iron -Soluble mg/1 0.03 0.06 0.55 0.05 (a) No data available.

TABLE B-6 PHYTOPLANKTON AND ZOOPLANKTON PRODUCTION IN THE COOLING LAKE OF WOLF CREEK GENERATING STATION, BURLINGTON, KANSAS, 1987 Phytoplankton Chlorophyll a Carbon Concentration Fixation Rate (mg/m3) (mg C/m3/hr)Date Location 2,3 MAR 2 6 8 Mean Dry Wei (mg/n Zooplankton Ash-Free.ght Dry Weight i3) (mg/m3)27,28 APR 22 JUN 24 AUG 23 NOV 28 DEC Annual Average 2 6 8 Mean 2 6 8 Mean 2 6 8 Mean 2 6 8 Mean 2 6 8 Mean 2 6 8 Mean 10.4 8.4 7.5 8.8 4.5 3.8 4.5 4.3 7.4 6.0 7.5 7.0 7.6 7.3 11.1 8.6 6.5 8.7 7.0 7.4 14.6 12.9 11.2 12.9 4.0 4.0 5.8 4.6 10.6 9.9 13.6 11.4 301 43 79 141 93 93 265 150 200 95 65 120 61 24 40 42 66 71 187 108 23 28 25 25 23 32 57 37 83 76 79 79 4.9 4.8 3.4 4.4 3.5 4.9 5.3 4.6 6.4 5.9 6.6 6.3 8.9 9.1 9.4 9.1 169 77 97 114 43 39 77 53 Note: Dash (-) indicates location not sampled.B-6 TABLE B-7 WOLF CREEK GENERATING STATION -1987 MACROINVERTEBRATE STUDY PONAR RESULTS: REPLICATE COUNTS AND MEAN DENSITIES FOR SPECIES DATE: 02MAR87 AND LOCATION:

2 REP A REP B MEAN COUNT COUNT (#/sq m) (M)SPECIES Dero digitata 1 0 9.4 3.85 Coenagrionidae

n. 1 0 9.4 3.85 Leptoceridae

1. 1 0 9.4 3.85 Chaoborus punctipennis

1. 0 1 9.4 3.85 Coelotanypus

1. 5 2 66.0 26.92 Chironomus

1. 2 7 84.9 34.62 Glyptotendipes

1. 6 0 56.6 23.08 TOTAL BENTHOS 16 10 245.3 100.00 DATE: 02MAR87 AND LOCATION:

4 REP A REP B MEAN COUNT COUNT (8/sq m) (M)SPECIES Branchiura sowerbyi 8 23 292.5 22.30 Limno. claparedianus 1 0 9.4 0.72 Limnodrilus hoffmeisteri 1 2 28.3 2.16 Imm. tub. w/o cap. chaet. 2 0 18.9 1.44 Isonychia

n. 2 0 18.9 1.44 Stenonema

n. 0 2 18.9 1.44 Cheumatopsyche

1. 1 0 9.4 0.72 Potamyia flava 1. 2 5 66.0 5.04 Stenelmis

1. 7 5 113.2 8.63 Chironomidae

p. 7 0 66.0 5.04 Orthocladiinae

1. 9 5 132.1 10.07 Cricot. tremulus grp. 1. 0 1 9.4 0.72 Eukiefferiella

1. 13 2 141.5 10.79 Hydrobaenus

1. 21 3 226.4 17.27 Orthocladius

1. 4 0 37.7 2.88 Chironominae

1. 2 1 28.3 2.16 Cryptochironomus

1. 1 0 9.4 0.72 Polypedilum scalaenum

1. 3 0 28.3 2.16 Corbicula 2 4 56.6 4.32 TOTAL BENTHOS 86 53 1311.3 100.00 TABLE B-7 (Cont.)DATE: 02MAR87 AND LOCATION:

6 REP A REP B COUNT COUNT SPECIES Deco Dero digitata Aulodrilus pigueti Chaoborus punctipennis 1.Chironomidae p.Tanypodinae 1.Procladius 1.Coelotanypus 1.Chironomus 1.TOTAL SENTHOS 1 0 10 0 3 0 2 5 1 0 MEAN (N/sq a)9.4 94.3 28.3 66.0 9.4 18.9 9.4 18.9 18.9 273.6 3.45 34.48 10.34 24.14 3.45 6.90 3.45 6.90 6.90 100.00 2 1 2 2 24 0 0 0 0 5 DATE: 02MAR87 AND LOCATION:

8 REP A REP B MEAN COUNT COUNT (#/sq m) (M)SPECIES Aulodrilus pigueti 1 0 9.4 11.11 Ilyodrilus templetoni 0 2 18.9 22.22 Imm. tub. w/ cap. chaet. 1 1 18.9 22.22 Coelotanypus

1. 0 3 28.3 33.33 Chironomus

1. 1 0 9.4. 11.11 TOTAL BENTHOS 3 6 84.9 100.00 DATE: 02MAR87 AND LOCATION:

10 I 00 REP A REP B COUNT COUNT MEAN (8/sq m)SPECIES Pristina lmm. tub. w/o cap. chaet.Enchytraeidae Stenonema n.Stenonema pulchellum n.Perlidae n.Neoperla n.Cheumatopsyche 1.Potamyia flare 1.Stenelmis 1.Chironomidae p.Orthocladiinae 1.Cricot. tremulus grp. 1.Eukiefferiella 1.Orthocladius 1.Hexatoma 1.Corbicula TOTAL BENTHOS 0 1 9.4 0 1 9 .4 1 0 9.4 6 3 84 .9 2 0 18.9 4 0 37.7 4 0 37.7 16 1 160 .4 46 9 518 .9 24 1 235.8 4 4 75.5 1 2 28.3 0 1 9.4 25 25 471.7 1 2 28 .3 2 1 28.3 2 0 18.9 138 51 1783.0 0.53 0.53 0.53 4.76 1.06 2.12 2.12 8.99 29.10 13.23 4.23 1.59 0.53 26.46 1.59 1.59 1.06 100.00 TABLE B-8 WOLF CREEK GENERATING STATION -PONAR RESULTS: REPLICATE COUNTS DATE: 27APR87 AND LOCATION:

2 1987 MACROINVERTEBRATE STUDY AND MEAN DENSITIES FOR SPECIES SPECIES Chaoborus punctipennis I.Coelotanypus 1.Chironomus 1.TOTAL BENTHOS REP A REP B MEAN COUNT COUNT (#/sq m)0 1 9.4 4 5 84.9 1 3 37.7 5 9 132.1 (9)7.14 64.29 28.57 100.00 DATE: 27APB87 AND LOCATION:

6 SPECIES Dero digitata Aulodrilus pigueti Ilyodrilus templetoni Limno. claparedianus Imm. tub. w/o cap. chaet.Imm. tub. w/ cap. chaet.Chaoborus punctipennis 1.Chironomidae p.tCoelotanypus 1.I Chironominae 1.1Chironomus 1.TOTAL BENTHOS REP A REP B COUNT COUNT 3 15 8 1 5 48 0 0 1 2 84 4 3 2 0 9 7 0 1 2 0 0 28 MEAN (t/sq m)66.0 169.8 94.3 9.4 132.1 518.9 9.4 9.4 18.9 9.4 18.9 1056.6 (M)6.25 16.07 8.93 0.89 12.50 49.11 0.89 0.89 1.79 0.89 1.79 100.00 DATE: 27APR87 AND LOCATION:

8 REP A REP B MEAN COUNT COUNT (#/sq m) (%)SPECIES Ilyodrilus templetoni 2 0 18.9 33.33 Coelotanypus

1. 2 0 18.9 33.33 Chironomus

1. 2 0 18.9 33.33 TOTAL BENTHOS 6 0 56.6 100.00 DATE: 28APR87 AND LOCATION:

4 REP A REP B COUNT COUNT SPECIES Hydra Imm. tub. w/o cap. chaet.Hydropsychidae i.Potamyia flava I.Cryptochironomus 1.Corbicula TOTAL BENTHOS 13 1 0 0 1 4 0 1 1 0 MEAN (#/sq m)160.4 9.4 9.4 9.4 9.4 9.4 207.5 77.27 4.55 4.55 4.55 4.55 4.55 100.00 1 0 16 6 TABLE B-8 lCont.)DATE: 28APR87 AND LOCATION:

10 REP A REP B MEAN COUNT COUNT (8/sq m) (%)SPECIES Hydra 12 4 150.9 66.67 Imm. tub. w/o cap. chaet. 0 1 9.4 4.17 Stenonoma

n. 0 1 9.4 4.17 Perlidae n. 1 0 9.4 4.17 Potamyia flava 1. 0 2 18.9 8.33 Elmidae 1. 0 1 9.4 4.17 Hexatoma 1. 1 0 9.4 4.17 Corbicula 0 1 9.4 4.17 TOTAL BENTHOS 14 10 226.4 100.00 I 0 TABLE B-9 WOLF CREEK GENERATING STATION -1987 MACROINVERTEBRATE STUDY PONAR RESULTS: REPLICATE COUNTS AND MEAN DENSITIES FOR SPECIES DATE: 22JUN87 AND LOCATION:

2 SPECIES Rexagenia a.Thienemanninyia ser. 1.Chironomus 1.TOTAL BENTHOS REP A REP B COUNT COUNT 0 0 2 2 I 1 0 2 MEAN (9/sq m)9.4 9.4 18.9 37.7 M50 25.00 25.00 50.00 100.00 DATE: 22JUN87 AND LOCATION:

4 REP A REP 8 MEAN COUNT COUNT (8/sq m) (%)SPECIES Ephoron album a. 1 2 28.3 18.75 Neoperla n. 1 1 18.9 12.50 Hydropsychidae

p. 1 0 9.4 6.25 Potamyia flava 1. 7 3 94.3 62.50 TOTAL BENTHOS 10 6 150.9 100.00 DATE: 22JUN87 AND LOCATION:

6 REP A REP B MEAN COUNT COUNT (8/sq m) (%)SPECIES Aulodrilus pigueti 1 3 37.7 66.67 Chironomus

1. 2 0 18.9 33.33 TOTAL BENTHOS 3 3 56.6 100.00 DATE: 22JUN87 AND LOCATION:

8 H REP A REP B COUNT COUNT MEAN (#/sq m)(%)SPECIES Chaoborus punctipennis 1.TOTAL BENTHOS 1 0 1 0 9.4 100.00 9.4 100.00 TABLE B-9 (Cont.)DATE: 22JUN87 AND LOCATION:

10 REP A REP B MEAN COUNT COUNT (#/sq m) (t)SPECIES Baetidae n. 0 1 9.4 2.33 Ephoron album n. 2 0 18.9 4.65 Neoperla n. 0 1 9.4 2.33 Hydropsychidae

p. 3 0 28.3 6.98 Potamyia flava 1. 21 13 320.8 79.07 Stenelmis

1. 1 0 9.4 2.33 Corbicula 0 1 9.4 2.33 TOTAL BENTHOS 27 16 405.7 100.00 I TABLE B-10 WOLF CREEK GENERATING STATION -1987 MACROINVERTEBRATE STUDY PONAR RESULTS: REPLICATE COUNTS AND MEAN DENSITIES FOR SPECIES DATE: 24AUG87 AND LOCATION:

2 SPECIES Procladius I.Coelotanypus 1.Chironomus 1.TOTAL BENTHOS REP A REP B COUNT COUNT 1 1 1 3 1 0 3 4 MEAN (#/sq m)18.9 37.7 9.4 66.0 28.57 57.14 14.29 100.00 DATE: 24AUG87 AND LOCATION:

4 REP A REP B MEAN COUNT COUNT (#/sq m) (M)SPECIES Glossiphoniidae 1 0 9.4 3.85 Neoperla n. I 0 9.4 3.85 Potamyia flava 1. 2 2 37.7 15.38 Stenelmis

1. 1 0 9.4 3.85 Hexatoma 1. 0 1 9.4 3.85 Gastropoda a I 9.4 3.85 Corbicula 3 14 160.4 65.38 TOTAL BENTHOS 8 18 245.3 100.00 DATE: 24AUG87 AND LOCATION:

6 REP A REP B MEAN COUNT COUNT (I/sq m) (M)SPECIES Coelotanypus

1. 1 0 9.4 50.00 Chironomus

1. 0 1 9.4 50.00 TOTAL BENTHOS 1 1 18.9 100.00 DATE: 24AUG87 AND LOCATION:

8 REP A REP B COUNT COUNT MEAN (#/sq m)(4)SPECIES No Organisms collected TOTAL BENTHOS a a 0 0 0.0 0.0 TABLE B-10 (Cont.)DATE: 24AUG87 AND LOCATION:

10 REP A REP B MEAN COUNT COUNT (#/sq m) (%)SPECIES Perlidae n. 0 1 9.4 7.69 Hydropsychidae

p. 2 3 47.2 38.46 Potamyia flays 1. 0 5 47.2 38.46 Cryptochironomus

1. 0 1 9.4 7.69 Corbicula 0 1 9.4 7.69 TOTAL BENTHOS 2 11 122.6 100.00 T TABLE B-11 WOLF CREEK GENERATING STATION -1987 MACROINVERTEBRATE STUDY PONAR RESULTS: REPLICATE COUNTS AND MEAN DENSITIES FOR FAMILIES DATE: 02MAR87 AND LOCATION:

2 REP A REP B COUNT COUNT FAMILY Naididae Coenagrionidas Leptoceridae Chaoboridae Chironomidae TOTAL BENTHOS 1 0 1 0 MEAN (#/sq m)9.4 9.4 9.4 9.4 207.5 245.3 (%)3.85 3.85 3.85 3.85 84.62 100.00 1 0 13 16 0 1 9 10 b;Ln DATE: 02MAR87 AND LOCATION:

4 REP A REP B MEAN COUNT COUNT (#/sq m) (%)FAMILY Tubificidae 12 25 349.1 26.62 Siphlonuridae 2 0 18.9 1.44 Heptageniidae 0 2 18.9 1.44 Hydropsychidae 3 5 75.5 5.76 Elmidae 7 5 113.2 8.63 Chironomidae 60 12 679.2 51.80 Corbiculidae 2 4 56.6 4.32 TOTAL BENTHOS 86 53 1311.3 100.00 DATE: 02MAR87 AND LOCATION:

6 REP A REP B MEAN COUNT COUNT (#/sq m) (%)FAMILY Naididae 11 0 103.8 37.93 Tubificidae 3 0 28.3 10.34 Chaoboridae 2 5. 66.0 24.14 Chironomidae 8 0 75.5 27.59 TOTAL BENTHOS 24 5 273.6 100.00 DATE: 02MAR87 AND LOCATION:

8 REP A REP B COUNT COUNT FAMILY Tubificidae Chironomidae TOTAL BENTHOS MEAN (#/sq m)47.2 37.7 84.9 2 1 3 3 3 6 (%)55.56 44.44 100.00 L~ C) ~22~mC)~Zi=~

TABLE B-li (Cont.)DATE: 02MAR87 AND LOCATION:

10 REP A REP B MEAN COUNT COUNT (#/sq m) (%)FAM4ILY Naididae 0 1 9.4 0.53 Tubificidae 0 1 9.4 0.53 Enchytraediae 1 0 9.4 0.53 Heptageniidae 8 3 103.8 5.82 Perlidae 8 0 75.5 4.23 fydropsychidae 62 10 679.2 38.10 Elmidae 24 1 235.8 13.23 Chironomidae 31 34 613.2 34.39 Simulidae 2 1 28.3 1.59 Corbiculidae 2 0 18.9 1.06 TOTAL BENTHOS 138 51 1783.0 100.00 as TABLE B-12 WOLF CREEK GENERATING STATION -1987 MACROINVERTEBRATE STUDY PONAR RESULTS: REPLICATE COUNTS AND MEAN DENSITIES FOR FAMILIES DATE: 27APR87 AND LOCATION:

2 REP A REP B COUNT COUNT FAMILY Chaoboridae Chironomidae TOTAL BENTHOS MEAN (#/sq m)9.4 122.6 132.1 0 1 5 8 5 9 (M)7.14 92.86 100.00 DATE: 27APR87 AND LOCATION:

6 REP A REP B MEAN COUNT COUNT (#/sq u) (M)FAMILY Naididae 3 4 66.0 6.25 Tubificidas 77 21 924.5 87.50 Chaoboridae 1 0 9.4 0.89 Chironomidas 3 3 56.6 5.36 TOTAL BENTHOS 84 28 1056.6 100.00 DATE: 27APR87 AND LOCATION:

8 REP A REP B MEAN COUNT COUNT (#/sq m) (M)FAMILY Tubificidae 2 0 18.9 33.33 Chironomidae 4 0 37.7 66.67 TOTAL BENTHOS 6 0 56.6 100.00 DATE: 28APR87 AND LOCATION:

4 REP A REP B COUNT COUNT MEAN (#/sq m)FAMILY Hydridae Tubificidae Hydropsychidae Chironomidae Corbiculidae TOTAL BENTHOS 13 4 160.4 1 0 9.4 0 2 18.9 1 0 9.4 1 0 9.4 16 6 207.5 (M)77.27 4.55 9.09 4.55 4.55 100.00 TABLE B-12 (Cont.)DATE: 28APR87 AND LOCATION:

10 REP A REP B MEAN COUNT COUNT (#/sq m) MV)FAMILY Hydridae 12 4 150.9 66.67 Tubificidae 0 1 9.4 4.17 Heptageniidae 0 1 9.4 4.17 Perlidae 1 0 9.4 4.17 Hydropsychidae 0 2 18.9 8.33 Elmidae 0 1 9.4 4.17 Simulidae 1 0 9.4 4.17 Corbiculidae 0 1 9.4 4.17 TOTAL BENTHOS 14 10 226.4 100.00 00 TABLE B-13 WOLF CREEK GENERATING STATION -1987 MACROINVERTEBRATE STUDY PONAR RESULTS: REPLICATE COUNTS AND MEAN DENSITIES FOR FAMILIES DATE: 22JUN87 AND LOCATION:

2 FAMILY Ephemeridas Chironomidae TOTAL BENTHOS REP A REP B COUNT COUNT MEAN (#/sq m)9.4 28.3 37.7 0 2 2 1 1 2 (2)25.00 75.00 100.00 luo I DATE: 22JUN87 AND LOCATION:

4 REP A REP B MEAN COUNT COUNT (#/sq m) (M)FAMILY Polymitarcyidae 1 2 28.3 18.75 Perlidae 1 1 18.9 12.50 Hydropsychidae 8 3 103.8 68.75 TOTAL BENTHOS 10 6 150.9 100.00 DATE: 22JUN87 AND LOCATION:

6 REP A REP B MEAN COUNT COUNT (#/sq m) (M)FAMILY Tubificidae 1 3 37.7 66.67 Chironomidae 2 0 18.9 33.33 TOTAL BENTHOS 3 3 56.6 100.00 DATE: 22JUN87 AND LOCATION:

8 REP A REP B MEAN COUNT COUNT (#/sq m) (M)FAMILY Chaoboridae 1 0 9.4 100.00 TOTAL BENTHOS 1 0 9.4 100.00 DATE: 22JUN87 AND LOCATION:

10 REP A REP B COUNT COUNT FAMILY Baetidao Polymitarcyidae Perlidae Hydropsychidae Elmidae Corbiculidae TOTAL BENTHOS 0 2 0 24 1 0 27 1 0 1 13 0 1 16 MEAN (#/sq m)9.4 18.9 9.4 349.1 9.4 9.4 405.7 2.33 4.65 2.33 86.05 2.33 2.33 100.00 TABLE B-14 WOLF CREEK GENERATING STATION -1987 MACROINVERTEBRATE STUDY PONAR RESULTS: REPLICATE COUNTS AND MEAN DENSITIES FOR FAMILIES DATE: 24AUG87 AND LOCATION:

2 REP A REP B COUNT COUNT MEAN (#/sq m)C')FAMILY Chironomidae TOTAL BENTHOS 3 4 66.0 100.00 3 4 66.0 100.00 W DATE: 24AUG87 AND LOCATION:

4 REP A REP B MEAN COUNT COUNT (#/sq m) MO)FAMILY Glossiphonidae 1 0 9.4 3.85 Perlidas 1 0 9.4 3.85 Hydropsychidae 2 2 37.7 15.38 Elmidae 1 0 9.4 3.85 Simulidae 0 1 9.4 3.85 Corbiculidas 3 14 160.4 65.38 Other Groups 0 1 9.4 3.85 TOTAL BENTHOS 8 18 245.3 100.00 DATE: 24AUG87 AND LOCATION:

6 REP A REP B MEAN COUNT COUNT (0/sq m) (M)FAMILY Chironomidae 1 1 18.9 100.00 TOTAL BENTHOS 1 1 18.9 100.00 DATE: 24AUG87 AND LOCATION:

8 REP A REP B MEAN COUNT COUNT (#/sq m) (M)FAMILY Hydridae 0 0 0.0 TOTAL BENTHOS 0 0 0.0 DATE: 24AUG87 AND LOCATION:

10 REP A REP B COUNT COUNT MEAN (#/sq m)FAMILY Perlidae Hydropsychidae Chironomidae Corbiculidae TOTAL BENTHOS 0 1 9.4 2 8 94.3 0 1 9.4 0 1 9.4 2 11 122.6 7.69 76.92 7.69 7.69 100.00 TABLE B-15 PHYSICAL MEASUREMENTS RECORDED DURING FISH SURVEYS IN THE NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION BURLINGTON, KANSAS, 1987 Reservoir (a) Discharge Water Temp (C)Date Gearat (cfs) 1 10 4 Conductivity (pmhos/cm) 1 10 4 Turbidity (N.T.U.)1 10 4 2 MAR EF 411 6.5 8.0 S 6.5 8.0 27 APR EF 554 21.5 22.0 S 21.5 22.0 22 JUN EF 316 29.0 29.0 S 29.0 29.0 24 AUG EF 752 22.0 23.0 S 22.0 23.0 (a) EF = electrofishing; S = seining.8.0 8.0 22.0 22.0 29.0 29.0 23.0 23.0 420 420 375 375 455 455 380 380 440 440 420 420 450 450 378 378 410 410 425 425 470 470 378 378 49 49 42 42 36 36 33 33 58 58 38 38 39 39 32 32 89 89 37 37 35 35 37 37 B-21 TABLE B-16

SUMMARY

OF ELECTROSHOCKING CATCH DATA FROM THE NEOSHO GENERATING STATION, BURLINGTON, KANSAS, 1987 Date/Location species Number CPE.(a)RIVER AND WOLF CREEK NEAR WOLF CREEK Total Length Imm)Mean Range Total Weight (g)Mean Range 2 MAR 1 10 4 I'.Gizzard shad Carp Red shiner River carpsucker Smallmouth buffalo Black bullhead White crappie Total Gizzard shad Carp Saallmouth buffalo Bigmouth buffalo Shorthead redhorse Channel catfish Freshwater drum Total Shortnose gar Carp Smallmouth buffalo Channel catfish Flathead catfish White crappie Freshwater drum Total Gizzard shad Carp River carpsucker Smallmouth buffalo Bigmouth buffalo Channel catfish Flathead catfish White bass White crappie Total Longnose gar Shortnose gar River carpsucker Smallmouth buffalo Longear sunfish Freshwater drum Total Longnose gar Carp River carpsucker Total 3 2 1 2 1 2 1 12 8 6 8 1 1 1 1 26 1 1 4 1 1 1 3 12 2 3 10 3 2 1 1 3 1 26 1 1 2 4 1 1 10 3.0 2.0 1.0 2.0 1.0 2.0 1.0 12.0 8.0 6.0 8.0 1.0 1.0 1.0 1.0 26.0 1.0 1.0 4.0 1.0 1.0 1.0 3.0 12.0 2.0 3.0 10.0 3.0 2.0 1.0 1.0 3.0 1.0 26.0 1.0 1.0 2.0 4.0 1.0 1.0 10.0 2.0 1.0 3.0 6.0 144 513 55 373 425 149 256 97 498 497 470 384 362 367 635 455 410 490 72 190 266 237 407 324 461 528 338 228 234 244 514 590 378 402 117 115 660 418 301 80-250 408-617 362-384 133-165 85-108 415-568 373-780 348-475 123-373 217-257 357-492 226-365 388-510 470-585 162-344 375-381 310-448 570-750 256-332 1900<10 550 1130 45 250<10 1397 1849 1790 640 350 580 660 1100 1050 1070<10 70 284 103 860 526 1503 310 120 275 120 315 800 755 1133 40 10<10-145 800-3000 520-580 30-60<10 1000-2100 670-5600 500-1950 18-500 70-135 260-1650 355-870 960-1870 56-700 730-780 420-1660 27 APR 1 10 4 2 1 3 6 675 400-950 1040 -355 205-460 TABLE B-16 (CONT)Total Length (mm) Total Weight (g)Date/Location Species Number CPE Mean Range Mean Range 22 JUM 1 Gizzard shad 255 25.0 49 42-60 <10 -Carp 4 4.0 310 227-357 425 150-600 Ghost shiner 1 1.0 42 -<10 -River carpsucker 5 5.0 265 228-350 280 170-580 Smallmouth buffalo 6 6.0 382 250-502 980 330-1710 Bigmouth buffalo 5 5.0 559 520-585 2814 1600-3940 Blue sucker 1 1.0 590 -1940 -Flathead catfish 3 3.0 217 140-302 140 30-290 White bass 3 3.0 246 27-357 <463 <10-730 Spotted bass 1 1.0 251 -225 -Freshwater drum 2 2.0 220 61-378 <400 (10-790 Total 286 286.0 10 River carpsucker 1 1.0 347 -630 -Bigmouth buffalo 1 1.0 475 -1240 -Channel catfish 1 1.0 665 -3540 -Flathead catfish 2 2.0 269 234-303 220 160-280 Total 5 5.0 4 Longnose (ar 1 1.0 605 -455 -Gizzard shad 3 3.0 339 60-770 (68 <10-150 Fathead minnow 1 1.0 73 -(10 -River carpsucker 3 3.0 296 142-379 458 35-700 Smallmouth buffalo 1 1.0 332 -510 -Flathead catfish 12 12.0 193 133-251 75 20-160 Longear sunfish 3 3.0 86 72-105 <15 <10-25 Spotted bass 1 1.0 137 White crappie 1 1.0 261 -250 -Total 26 26.0 24 AUG 1 Carp 4 4.0 363 312-445 650 450-1050 River carpsucker 2 2.0 297 273-320 324 248-400 Smallmouth buffalo 3 3.0 393 325-511 1073 520-2050 Bigmouth buffalo 1 1.0 585 -3400 -Blue sucker 1 1.0 670 -2960 -Channel catfish 1 1.0 408 -530 -Flathead catfish 2 2.0 228 225-230 116 108-123 Green sunfish 2 2.0 58 52-63 (10 -Orangespotted sunfish 1 1.0 63 -(10 -Spotted bass 1 1.0 225 -136 -White crappie 1 1.0 94 -(10 -Freshwater drum 5 5.0 185 78-424 <283 <10-1270 Total 24 24.0 10 Longnose gar 1 1.0 602 -570 -Channel catfish 1 1.0 320 -270 -Green sunfish 1 1.0 89 Longear sunfish 2 2.0 82 76-88 <12 (10-13 Freshwater drum 7 7.0 156 87-255 <71 (10-220 Total 12 12.0 TABLE B-16 (CONT)Date/Location 4 Species Gizzard shad River carpaucker Smallmouth buffalo Flathead catfish Longear sunfish white crappie Freshwater drum Total Number CPE Total Length (mm)Mean Range 5 3 1 2 1 4 17 5.0 3.0 1.0 2.0 1.0 1.0 4.0 17.0 90 352 418 241 83 281 165 87-91 301-391 222-260 103-344 Total Weight (g)Mean Range<10 -600 350-750 140 100-180 11 330 140 (10-530 (a) CPE represents number of fish collected per 30-minute effort.Is TABLE B-17

SUMMARY

OF SEINE DATA FROM THE KANSAS, 1987 NEOSHO RIVER NEAR WOLF CREEK GENERATING STATION, BURLINGTON, Date/Location 2 MAR 1 Species Gizzard Shad Ghost shiner Total Number 1 3 4 Total Length (mm)Mean Range 88 -35 32-40 Total Weight (g)Mean Range-<10-<10 10 4 11 Ghost shiner Red Shiner Mosquitofish Total 94 34 2 130 28 35 33 19-39 21-51 23-42 (10 (10<10 27 APR 1 I U'10 Sample Not Preserved No Neosho madtoms Ghost shiner Red shiner Brook silverside White crappie Total Ghost shiner Red shiner Slim minnow Mosquitofish Brook silverside Total Ghost shiner Red shiner Slim minnow Channel catfish Green sunfish Total No Neosho madtoms Gizzard shad Carpiodes sp.Ghost shiner Mosquitofish Brook silverside White bass Spotted bass White crappie Total Gizzard shad Red shiner Suckermouth minnow 7 30 1 2 40 33 26-37 41 29-55 73 107 102-112 13 30-40 Minnows 39 345 1 5 1 391 173 31 2 2 1 209 74 10 1 5 1 6 1 17 115 11 22 JUN 1 10 31 39 27 32 43 34 32 32 68 63 51 48 46 44 23 53 54 46 68 39 47 20-41 22-63 27-38 22-44 21-49 27-37 58-77<10 110 (:10 12-14<10 (10<10 (10<10 (10 (10<10 (10<10<10 (10 (10<10<10 (10<10 (10<10<10 (10<10 25-75 44-53 38-52 43-67 36-59 64-73 42-60 45-48 5 25 2 TABLE B-17 (CONT)Total Length (mm) Total Weight (g)Date/Location Species Number Mean Range Moan Range 10 (Cont.) Slim minnow 2 50 47-52 -<10 Channel catfish 1 132 Black-striped topminnow 2 28 23-33 -<10 Mosquitofish 14 26 21-32 -(10 White bass 4 47 36-58 -<10 Freshwater drum 1 58 --<10 Total 56 11 Neosho madtom 1 27 --<10 4 Gizzard shad 69 46 35-72 -<10 Ghost shiner 167 39 17-48 -(10 Red shiner 15 48 34-68 -(10 Suckermouth minnow 2 37 36-37 -<10 Slim minnow 1 22 --<10 Mosquitofish 17 27 23-44 -(10 White bass 8 42 35-48 -<10 Spotted bass 1 56 --(10 White crappie 6 36 27-41 -<10 Orangethroated darter 1 25 --<10 Total 287 24 AUG 1 Gizzard shad 3 89 87-91 -<10 Ghost shiner 19 22 20-26 -<10 Carpiodes sp. 1 78 --<10 Mosquitofish 1 47 --<10 Brook silverside 2 36 25-36 -(10 Green sunfish 2 25 23-26 -<10 Orangespotted sunfish 1 42 --<10 White crappie 10 86 77-91 -(10 Total 39 10 Red shiner 60 36 20-54 -<10 Suckermouth minnow 3 60 52-71 -<10 Bluntnose minnow 5 46 39-57 -(10 Bullhead minnow 2 35 31-39 -<10 Channel catfish 2 66 61-70 -(10 Noturus sp. 1 18 --(10 M'squitofish 122 27 16-48 -(10 Orangethroated darter 4 46 37-65 -<10 Total 199 4 Ghost shiner 162 32 18-50 -(10 Red shiner 62 32 23-50 -(10 Notropis sp. 1 (20 --<10 Bluntnose minnow 8 42 35-51 -(10 Bullhead minnow 11 41 30-50 -(10 Pimephales op. 42 41 --<10 Mosquitofish 151 25 16-42 -<10 TABLE B-17 (CONT)Date/Location 4 (Cont.)Species Orangespotted sunfish Lepomis sp.White crappie Total No Neosho Madtom Number 4 16 1 458 Total Length (mm)Mean Range 45 44-47 22 18-24 64 -Total Weight (g)Mean Range-(10-<10-<10 11 I t',,3 ,,,,,j Enclosure 4 to ET 07-0001 Chemistry Effluent Monitoring Data Item 1 -Figure & Map of NPDES Outfall Sampling Points (small & large area)Item 2 -Table of WCNOC Results from November 1, 2006 -November 30, 2006 Item 3 -Analytical Results from Accredited Environmental Laboratory Item 4 -Circulating Water Bromination Schedule Item 5 -Service Water Bromination Schedule Item 6 -Logs for Discharges through outfall 003A & 003B Item 1 of Enclosure 4 to Letter ET 07-0001 Figure & Map of NPDES Outfall Sampling Points (small & large area)

I SRevision:

13 CHS NP-LO1 NPDES OUTFALLS SAMPLING AND FLOW 1 Continuous Use CALCULATIONS Page 16 of 16 t[ FIGURE 2 NPDES OUTFALL PROGRAM 005o 002a- 0WAteer System Discharge 002 b- Discharge of Settlig Basin ito Wolf Creek Cooing tmpoundmret 003- Clrculation Water System Discharge 003a- Radwaste System Discharge 003b- Wastewater Tredmenrt Fmilty Discharge 004. -Cooing mpToundImert Discharge 005. -LUme Slukge Pond 006a -Essertlal Service Water System ischmarge N" W&003 I Oro. Watur nalke Wolf Creek Cooing Iump rndment Page 14 L. Kansas Permnit No.: I-NE07-PO02 ATTACHMENT C -WOLF CREEK POWER PLANT. BURLINGTON

-OUTFALLS LOCATION MAP 0 EISENHOWERLEARNINGOCENTER q '* SCELL DOMESTIC WASTEWATER " " ' .6 o ~~STASflZAT1OW

, 0 ..I-* ., **.A.,"I ..-.' j " il4(U8,,.onc.*.

-.,~ -< ....ca A.0 I4*~MKU WAE ONACU.L~~c I ..-.I -' " "!"I01S PFIv UP Lim c-A .m 4.* 5 *--" I~t t ILi* * , , " .Zs~ *,,, W16 ' I " ROI.. : ' ,X OS ',';I ..A. 'o Item 2 of Enclosure 4 to Letter ET 07-0001 Table of WCNOC Results from November 1, 2006 -November 30, 2006 L OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1 Sample Point: OILY -OUTFALL 002 TITLE DESCRIPTOR Group -SHOP System -OILY Analysis FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD" FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOWIMGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOWIMGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD Value/Meas.

Unit 0 MOD 0 MOD 0 MOD 0 MOD 0 MGD 0 MGD 0 MOD 0 MOD 0 MGD 0 MOD Limits Exceeded Datef/ime 01-Nov-2006 08:40:00 02-Nov-2006 09:00:00 03-Nov-2006 08:35:00 04-Nov-2006 08:17:00 05-Nov-2006 09:40:00 06-Nov-2006 08:35:00 07-Nov-2006 08:35:00 07-Nov-2006 08:53:00 08-Nov-2006 09:28:00 09-Nov-2006 08:10:00 Power or 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Mode 5 5 5 5 5 5 4 4 3 3 Anal 0 MOD 10-Nov-2006 08:50:00 5.00 1 0 MOD I"1Nov'2006v08:55:00 5.00 1 0 MGD 12-Nov-2006'08:52:00 100.00 1 0 MGD 12-Nov-2006 09:20:00 100.00 1 0 MOD 13-Nov-2006 08:52:00 100.00 1 0 MOD 13-Nov-2006 09:20:00 100.00 1 0 MGD 14-Nov-2006 09:26:00 100.00 1 0.851 MOD 4- w Ax.t 15-Nov-2006 08:25:00 100.00 1 0 MOD 16-Nov-2006 08:50:00 100.00 1 0 MOD 17-Nov-2006 08:57:00 100.00 1 TIL ESB ESB TrL 3LH TTL TrL MC TTL TI'L ESB ESB ESB ESB ESB JWB TTL TrL rDW rDW-DG[SM rSM BLH ESB MC ALD TaL 0 MOD 0 MGD 0 MOD 0 MGD 0 MGD 0 MOD 0 MGD 0 MGD 0 MGD 0 MGD 18-Nov-2006 08:50:00 19-Nov-2006 09:05:00 20-Nov-2006 08:35:00 21-Nov-2006 11:10:00 22-Nov-2006 09:00:00 23-Nov-2006 09:00:00 24-Nov-2006 09:38:00 25-Nov-2006 08:30:00 26-Nov-2006 08:45:00 27-Nov-2006 09:03:00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 1 1 1 1 1 1 1 I 1 1 N N (1~FLO FLC FLC FO)FO)FO)FOA O/G PH TSS 0R%)W/MGD 2.79E-02 MGD ) 28-Nov-2006 09:12:00 100.00 1 CDG)W/MGD 7.13E-04 MOD k, Sk.cev" Wa-. 29-Nov-2006 09:34:00 100.00 1 CDG)W/MGD 1.83e-02 MGD 30-Nov-2006 09:10:00 100.00 1 TTL 0 15-Nov-2006 08:25:00 100.00 1 JWB 0 23-Nov-2006 09:00:00 100.00 1 BLH LM 0 28-Nov-2006 09:12:00 100.00 1 CDG w 0 29-Nov-2006 09:34:00 100.00 1 CDG 0 30-Nov-2006 09:10:00 100.00 1 TTL< 1.0 PPM 16-Nov-2006 08:50:00 100.00 1 TTL 8.0 PH 16-Nov-2006 08:50:00 100.00 1 TaL A-1 SACT 9.4 PPM 0 UCI/CC 16-Nov-2006 08:50:00 16-Nov-2006 08:50:00 100.00 100.00 1 1 TaL TaL Report Generated:

12/14/2006 11:55 Page 1 of 2 Report Generated:

12/14/2006 11:55 Page I of 2 I OpenCDM A1 Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek El[:1 From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1 Sample Point: OILY -OUTFALL 002 TITLE DESCRIPTOR Group- SHOP o System -OILY Analysis Value/Meas.

Unit Limits Exceeded Date/rime Power OpMode Analyst H-3 i.875e-06 UCI/CC 16-Nov-2006 08:50:00 100.00 1 T-- L TOTAL NUMBER OF MEASUREMENTS:

43 1 END OF MEASUREMENTS REPORT Report Generated:

12/14/2006 11:55 Page 2 of 2 Report Generated:

12/14/2006 11:55 Page 2 of 2 I E:[)OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit I 2 Sample Point: CIRC -CIRCULATING WATER DISCHARGE TITLE DESCRIPTOR Group- SHOP I. Sysem -CIRC SAnalvis Value/Meas.

Unit Limits Exceeded Date/Timi e FOAM FOAM FOAM FOAM FOAM FOAM FOAM FOAM FOAM FOAM 0 0 0 0 0 0 0 0 0 0 01 -Nov-2006 08:45:00 02-Nov-2006 09:02:00 03-Nov-2006 08:40:00 04-Nov-2006 08:21:00 05-Nov-2006 09:38:00 06-Nov-2006 08:45:00 07-Nov-2006 08:55:00 08-Nov-2006 09:25:00 09-Nov-2006 08:15:00 10-Nov-2006 08:52:00 Power 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 5.00 OpMode 5 5 5 5 5 5 4 3 3 1 Analyst TrL TFL ESB ESB TrL MC TrL MC TML FOAM 0 1 1-Nov-2006 09:00:00.

5.00 1 TTL FOAM 0 12-Nov-2006 08:57:00 % 100.00 1 ESB FOAM 0 12-Nov-2006 09:25:00.

.100.00 1 ESB FOAM 0 13-Nov-2006 08:57:00 100.00 1 ESB FOAM 0 13-Nov-2006 09:25:00 100.00 1 ESB FOAM 0 14-Nov-2006 09:28:00 100.00 1 TL FOAM 0 15-Nov-2006 08:30:00 100.00 1 JWB FOAM 0 16-Nov-2006 09:00:00 100.00 1 iTIL FOAM 0 17-Nov-2006 09:00:00 100.00 1 TL FOAM 0 18-Nov-2006 09:05:00 100.00 1 NDW FOAM FOAM FOAM FOAM FOAM FOAM FOAM FOAM FOAM FOAM FOAM FOAM FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC 0 1 0 0 0 0 0 0 0 0 0 0 0.02 0.02< 0.01< 0.01 0 .01 0.02 0.01 0.02 0.02 0.01 19-Nov-2006 09:20:00 20-Nov-2006 08:39:00 21-Nov-2006 11:15:00 22-Nov-2006 09:05:00 23-Nov-2006 09:13:00 24-Nov-2006 09:40:00 25-Nov-2006 08:35:00 26-Nov-2006 08:50:00 27-Nov-2006 09:00:00 28-Nov-2006 09:15:00 29-Nov-2006 09:36:00 30-Nov-2006 09:15:00 01-Nov-2006 08:45:00 02-Nov-2006 09:02:00 03-Nov-2006 08:40:00 04-Nov-2006 08:21:00 05-Nov-2006 09:38:00 06-Nov-2006 08:45:00 07-Nov-2006 08:55:00 08-Nov-2006 09:25:00 09-Nov-2006 08:15:00 1 0-Nov-2006 08:52:00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 5.00 5 5 1 1 1 1 1 1 1 1 1 5 5 5 5 4 3 3 NDW CDG TSM TSM BLH ESB MC MLD TaL CDG CDG TrL TTL TrL ESB ESB TrL MC TTL TTL MC TTL PPM PPM PPM PPM PPM PPM PPM PPM PPM PPM Report Generated:

12/05/2006 11:05 Page 1 of 7 Report Generated:

12/05/2006 11:05 Page I of 7 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 I 2.Unit: 1 -Wolf Creek Unit 1 2 Sample Point: CIRC -CIRCULATING WATER DISCHARGE TITLE DESCRIPTOR Group -SHOP System -CIRC (:J 0 Anal FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC FAC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC vsh Value/Meas.

Unit 0.02 PPM< 0.01 PPM< 0.01 PPM Limits Exceeded Date/Time I1 -Nov-2006 09:00:00 12-Nov-2006 08:57:00 12-Nov-2006 09:25:00 Power 5.00 100.00 100.00 OpMode I I I Analyst TrL ESB ESB< 0.01 PPM 13-Nov-2006 08:57:00 100.00 1 ESB< 0.01 PPM 13-Nov-2006 09:25:00 100.00 1 ESB 0.04 PPM 14-Nov-2006 09:28:00 100.00 1 TTL< 0.01 PPM 15-Nov-2006 08:30:00 100.00 1 JWB< 0.01 PPM 16-Nov-2006 09:00:00 100.00 1 TIM 0.01 PPM 17-Nov-2006 09:00:00 100.00 1 TTL 0.03 PPM 18-Nov-2006 09:05:00 100.00 1 NDW 0.01 PPM 19-Nov-2006 09:20:00 100.00 1 NDW< 0.01 PPM 20-Nov-2006 08:39:00 100.00 1 CDG< 0.01 PPM 21-Nov-2006 11:15:00 100.00 1 TSM< 0.01 PPM 22-Nov-2006 09:05:00 100.00 1 TSM< 0.01 PPM 23-Nov-2006 09:13:00 100.00 1 BLH< 0.01 PPM 24-Nov-2006 09:40:00 100.00 1 ESB< 0.01 PPM 25-Nov-2006 08:35:00 100.00 1 MC< 0.01 PPM 26-Nov-2006 08:50:00 100.00 1 MLD 0.01 PPM 27-Nov-2006 09:00:00 100.00 1 TIL< 0.01 PPM 28-Nov-2006 09:15:00 100.00 1 CDG< 0.01 PPM 29-Nov-2006 09:36:00 100.00 1 CDG< 0.01 PPM 30-Nov-2006 09:15:00 100.00 1 TTL 0.09 PPM 01-Nov-2006 08:45:00 0.00 5 1TL 0.05 PPM 02-Nov-2006 09:02:00 0.00 5 TTL 0.05 PPM 03-Nov-2006 08:40:00 0.00 .5 ESB 0.05 PPM 04-Nov-2006 08:21:00 0.00 5 ESB< 0.01 PPM 05-Nov-2006 09:38:00 0.00 5 TTL 0.06 PPM 06-Nov-2006 08:45:00 0.00 5 MC 0.06 PPM 0.08 PPM 0.06 PPM 0.06 PPM 0.04 PPM 0.04 PPM 0.03 PPM 0.04 PPM >0.03 PPM 0.08 PPM 0.05 PPM 0.05 PPM 0.07 PPM 0.07 PPM 07-Nov-2006 08:55:00 08-Nov-2006 09:25:00 09-Nov-2006 08:15:00 10-Nov-2006 08:52:00 1-1 -Nov-2006 09:00:00 12-Nov-2006 08:57:00 12-Nov-2006 09:25:00 13-Nov-2006 08:57:00 13-Nov-2006 09:25:00 14-Nov-2006 09:28:00 15-Nov-2006 08:30:00 16-Nov-2006 09:00:00 1 7-Nov-2006 09:00:00 18-Nov-2006 09:05:00 0.00.0.00 0.00 5.00 5.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 4 3 3 1 I I 1 1 1 TIrL MG TIL TTL ESB ESB ESB ESB TrL JWB YTL TrL NDW Report Generated:

12/05/2006 11:05 Page 2 of 7 Report Generated:

12/05/2006 11:05 Page 2 of 7 E:[1)OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 1 4.4.1.Unit: 1 -Wolf Creek Unit 1 Sample Point: CIRC -CIRCULATING WATER DISCHARGE TITLE DESCRIPTOR Group -SHOP System -CIRC~;Anal TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC-TRC.TRC TRC-TRC-TC-VMS Value/Mess.

Unit 0.07 PPM 0.06 PPM< 0.01 PPM< 0.01 PPM< 0.01 PPM 0.05 PPM Limits Exceeded Date/Time 19-Nov-2006 09:20:00 20-Nov-2006 08:39:00 21-Nov-2006 11:15:00 22-Nov-2006 09:05:00 23-Nov-2006 09:13:00 24-Nov-2006 09:40:00 Power 100.00 100.00 100.00 100.00 100.00 100.00 ODMode I I I 1 I 1 Analys NDW CDG TSM TSM BLH ESB 0.05 PPM 25-Nov-2006 08:35:00 100.00 1 MC 0.05 PPM 26-Nov-2006 08:50:00 100.00 1 MLD 0.05 PPM 27-Nov-2006 09:00:00 100.00 1 TTL< 0.01 PPM 28-Nov-2006 09:15:00 100.00 1 CDG< 0.01 PPM 29-Nov-2006 09:36:00 100.00 1 CDG 0.06 PPM 30-Nov-2006 09:15:00 100.00 1 TTL-DUP 0.09 PPM 01-Nov-2006 08:45:00.

0.00 5 TrL-DUP 0.05 PPM 02-Nov-2006 09:02:00 0.00 5 TTL-DUP 0.05 PPM 03-Nov-2006 08:40:00 0.00 5 ESB-DUP 0.05 PPM 04-Nov-2006 08:21:00 0.00 5 -ESB-DUP < 0.01 PPM 05-Nov-2006 09:38:00 0.00 5 TTL-DUP 0.06 PPM 06-Nov-2006 08:45:00 0.00 5 MC-DUP 0.06 PPM 07-Nov-2006 08:55:00 0.00 4 TlL-DUP 0.08 PPM 08-Nov-2006 09:25:00 0.00 3 TTL.DUP 0.06 PPM 09-Nov-2006 08:15:00 0.00 3 MC-DUP*DUP DUP-DUP DUP DUP DUP DUP-DUP-DUP-DUP-DUP-DUP-DUP-DUP-DUP-DUP-DUP-DUP-DUP 0.06 PPM 0.04 PPM 0.04 PPM 0.03 PPM 0.04 PPM 0.03 PPM 0.08 PPM 0.05 PPM 0.05 PPM 0.07 PPM 0.07 PPM 0.07 PPM 0.06 PPM< 0.01 PPM< 0.01 PPM< 0.01 PPM 0.05 PPM 0.05 PPM 0.05 PPM 0.05 PPM< 0.01 PPM 10-Nov-2006 08:52:00 1 1-Nov-2006 09:00:00 12-Nov-2006 08:57:00 12-Nov-2006 09:25:00 13-Nov-2006 08:57:00 13-Nov-2006 09:25:00 14-Nov-2006 09:28:00 15-Nov-2006 08:30:00 16-Nov-2006 09:00:00 17-Nov-2006 09:00:00 18-Nov-2006 09:05:00 19-Nov-2006 09:20:00 20-Nov-2006 08:39:00 21-Nov-2006 11:15:00 22-Nov-2006 09:05:00 23-Nov-2006 09:13:00 24-Nov-2006 09:40:00 25-Nov-2006 08:35:00 26-Nov-2006 08:50:00 27-Nov-2006 09:00:00 28-Nov-2006 09:15:00 5.00 5.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 1 1 1 1 1 1 I I 1 1 1 I 1 I I I 1 I 1 I I TTL ESB ESB ESB ESB TTL JWB TYrL TrL NDW NDW CDG TSM TSM BLH ESB MC MLD YTL CDG Report Generated:

12/05/2006 11:05 Page 3 of 7 Report Generated:

12/05/2006 11:05 Page 3 of 7 I ri p.F OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 I/Unit: 1 -Wolf Creek Unit I 21 Sample Poix 9 F Analys.TRC-DUP TRC-DUP%D-TRC%D-TRC%/oD-TRC%D-TRC%D-TRC%D-TRC%D-TRC%D -TRC%D-TRC%D-TRC%D-TRC%D-TRC%D-TRC%D-TRC%D-TRC%D-TRC%D-TRC%D-TRC%D-TRC%D-TRC%D.-TRC%D-TRC%D-TRC%D-TRC%D-TRC%/oD-TRC%/oD-TRC%D-TRC%D-TRC%D-TRC%D-TRC%D-TRC FLOW/MGD FLOW/MGD FLOW/l'GD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD at: CIRC -CIRCULATING WATER DISCHARGE TITLE DESCRIPTOR Group -SHOP System -CIRC Value/Meas.

Unit< 0.01 PPM 0.06 PPM 0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%0%00%0%0%0%0%558 MGD 558 MGD 564 MGD 564 MGD 564 MGD 565 MGD 567 MGD 567 MGD Limits Exceeded Datefrime 29-Nov-2006 09:36:00 30-Nov-2006 09:15:00 01-Nov-2006 08:45:00 02-Nov-2006 09:02:00 03-Nov-2006 08:40:00 04-Nov-2006 08:21:00 05-Nov-2006 09:38:00 06-Nov-2006 08:45:00 07-Nov-2006 08:55:00 08-Nov-2006 09:25:00 09-Nov-2006 08:15:00 10-Nov-2006 08:52:00.I l-Nov-2006 09:00:00,! 2-Nov-2006 08:57:00 12-Nov-2006 09:25:00! 3-Nov-2006 08:57:00 13-Nov-2006 09:25:00 14-Nov-2006 09:28:00 15-Nov-2006 08:30:00 16-Nov-2006 09:00:00 17-Nov-2006 09:00:00 18-Nov-2006 09:05:00 19-Nov-2006 09:20:00 20-Nov-2006 08:39:00 21-Nov-2006 11:15:00 22-Nov-2006 09:05:00 23-Nov-2006 09:13:00 24-Nov-2006 09:40:00 25-Nov-2006 08:35:00 26-Nov-2006 08:50:00 27-Nov-2006 09:00:00 28-Nov-2006 09:15:00 29-Nov-2006 09:36:00 30-Nov-2006 09:15:00 01-Nov-2006 08:45:00 02-Nov-2006 09:02:00 03-Nov-2006 08:40:00 04-Nov-2006 08:21:00 05-Nov-2006 09:38:00 06-Nov-2006 08:45:00 07-Nov-2006 08:55:00 08-Nov-2006 09:25:00 Power OpMode 100.00 1 100.00 1 0.00 5 0.00 5 0.00 5 0.00 5 0.00 5 0.00 5 0.00 4 0.00 3 0.00 3.5.00 1'5.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 0.00 5 0.00 5 0.00 5 0.00 5 0.00 5 0.00 5 0.00 4 0.00 3 Analyst CDG TI'L TTL TTL ESB ESB TTL MC TTL TTL MC* ESB ESB ESB BLH TM'JWB 7TL TrL NDW NDW CDG TSM TSM BLH ESB MC MLD TTL CDG CDG TTL TRL YTL ESB ESB TTL MC TrL TTL Report Generated:

12/05/2006 11:05 Page 4 of 7 Report Generated:

12/05/2006 11:05 Page 4 of 7 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: I -Wolf Creek Unit 1ý' Sample Point: CIRC -CIRCULATING WATER DISCHARGE 9 TITLE DESCRIPTOR Group -SHOP System -CIRC 0 E Analysis Value/Meas.

Unit Limits Exceeded DatefTime Power OpMode Analyst FLOW/MGD 567 MGD 09-Nov-2006 08:15:00 0.00 3 MC FLOW/MGD 569 MGD l0-Nov-2006 08:52:00 5.00 1 TIT, FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOWIMGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD*.FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD PH PH PH PH PH PH PH PH PH PH PH PH PH PH PH PH PH PH 573 MGD 573 MGD 576 MGD 573 MGD 576 MGD 574 MGD 577 MGD 573 MGD 573 MGD 571 MGD 571'MGD 574 MGD 580 MGD 555 MGD 573 MGD 573 MGD 577 MGD 577 MGD 576 MGD 554 MGD 554 MGD 549 MGD 8.4 PH 8.5 PH 8.3 PH 8.3 PH 8.4 PH 8.5 PH 8.4 PH 8.4 PH 8.4 PH 8.4 PH 8.4 PH 8.2 PH 8.5 PH 8.2 PH 8.5 PH 8.4 PH 8.4 PH 8.4 PH I I-Nov-2006 09:00:00 12-Nov-2006 08:57:00 12-Nov-2006 09:25:00 13-Nov-2006 08:57:00 13-Nov-2006 09:25:00 14-Nov-2006 09:28:00 15-Nov-2006 08:30:00 16-Nov-2006 09:00:00 17-Nov-2006 09:00:00 1 8-Nov-2006 09:05:00 19-Nov-2006 09:20:00 20-Nov-2006 08:39:00 21-Nov-2006.11:15:00 22-Nov-2006 09:05:00 23-Nov-2006 09:13:00 24-Nov-2006 09:40:00 25-Nov-2006 08:35:00 26-Nov-2006 08:50:00 27-Nov-2006 09:00:00 28-Nov-2006 09:15:00 29-Nov-2006 09:36:00 30-Nov-2006 09:15:00 0 1-Nov-2006 08:45:00 02-Nov-2006 09:02:00 03-Nov-2006 08:40:00 04-Nov-2006 08:21:00 05-Nov-2006 09:38:00 06-Nov-2006 08:45:00 07-Nov-2006 08:55:00 08-Nov-2006 09:25:00 09-Nov-2006 08:15:00 10-Nov-2006 08:52:00 1 1-Nov-2006 09:00:00 12-Nov-2006 08:57:00 12-Nov-2006 09:25:00 13-Nov-2006 08:57:00 13-Nov-2006 09:25:00 14-Nov-2006 09:28:00 15-Nov-2006 08:30:00 16-Nov-2006 09:00:00 5.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00.100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 5.00 5.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 1 1 I I 1 1 1 1 1 1 1 1 1 1 I 1 1 I 1 5 5 5 5.5 5 4 3 3 1 1 1 1 1 TrL ESB ESB ESB ESB TaL JWB TrL TrL NDW NDW CDG TSM TSM BLH ESB MC MLD TrL CDG CDG I'M TTL TrL ESB ESB TrL MC TrL TrL Mc TrL ESB ESB ESB ESB TaL JWB TaL Report Generated:

12/05/2006 11:05 Page 5 of 7 Report Generated:

12/05/2006 11:05 Page 5 of 7 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 i Unit: 1 -Wolf Creek Unit 1-. Sample Point: CIRC -CIRCULATING WATER DISCHARGE TITLE DESCRIPTOR Group -SHOP 0 System -CIRC 0J G; Analysis PH PH PH PH PH PH PH PH PH PH PH PH PH PH PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUJP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP Value/Meas.

Unit 8.4 PH 8.5 PH 8.5 PH 8.4 PH 8.1 PH 8.4 PH 8.5 PH 8.3 PH 8.4 PH 8.4 PH 8.4 PH 8.5 PH 8.6 PH 8.3 PH 8.4 PH 8.5 PH 8.3 PH 8.3 PH 8.5 PH 8.5 PH 8.4 PH 8.4 PH 8.4 PH 8.4 PH 8.4 PH 8.2 PH 8.5 PH 8.2 PH 8.5 PH 8.4 PH 8.4 PH 8.4 PH 8.4 PH 8.5 PH 8.5 PH 8.4 PH 8.1 PH 8.4 PH 8.5 PH 8.3 PH 8.4 PH 8.4 PH Limits Exceeded Datetrime 17-Nov-2006 09:00:00 18-Nov-2006 09:05:00 19-Nov-2006 09:20:00 20-Nov-2006 08:39:00 21-Nov-2006 11:15:00 22-Nov-2006 09:05:00 23-Nov-2006 09:13:00 24-Nov-2006 09:40:00 25-Nov-2006 08:35:00 26-Nov-2006 08:50:00 27-Nov-2006 09:00:00 28-Nov-2006 09:15:00 29-Nov-2006 09:36:00 30.Nov-2006 09:15:00 01-Nov-2006 08:45:00 02-Nov-2006 09:02:00 03-Nov-2006 08:40:00 04-Nov-2006 08:21:00 05-Nov-2006 09:38:00 06-Nov-2006 08:45:00 07-Nov-2006 08:55:00 08-Nov-2006 09:25:00 09-Nov-2006 08:15:00 10-Nov-2006 08:52:00 1 1-Nov-2006 09:00:00 12-Nov-2006 08:57:00 12-Nov-2006 09:25:00 1 3-Nov-2006 08:57:00 13-Nov-2006 09:25:00 14-Nov-2006 09:28:00 15-Nov-2006 08:30:00 16-Nov-2006 09:00:00 17-Nov-2006 09:00:00 18-Nov-2006 09:05:00 19-Nov-2006 09:20:00 20-Nov-2006 08:39:00 21-Nov-2006 11:15:00 22-Nov-2006 09:05:00 23-Nov-2006 09:13:00 24-Nov-2006 09:40:00 25-Nov-2006 08:35:00 26-Nov-2006 08:50:00 Power 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 5.00 5.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 ODMode 5 1 5 1 1 1 1 1 5 5 4 3 3 1.1 1 1 5 1 1 1 5 4 3 1 1 1 1 Analyst TrL NDW NDW CDG TSM TSM BLH ESB MC MLD TrL CDG CDG TrL TrL mIL ESB ESB TM MC TM TrL MC TTL TrL ESB ESB ESB ESB TTL JWB TL TTL NDW NDW CDG TSM TSM BLH ESB MC MLD Report Generated:

12/05/2006 11:05 Page 6 of 7 Report Generated:

12/05/2006 11:05 Page 6 of 7 I M'OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unt Unit: 1 -Wolf Creek Unit 1 I Sample Point: CIRC -CIRCULATING WATER DISCHARGE I TITLE DESCRIPTOR Group- SHOP System -CIRC L;E;Analysi Value/Meas.

Unit PH-DUP 8.4 PH PH-DUP 8.4 PH PH-DUP 8.6 PH PH-DUP 8.3 PH%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %/oD-pH 1.2 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH. 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0%%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 0 %%D-pH 1.2 %%D-pH 0 %%D-pH 0 %CAHARD 124 PPM CACO3 TALK 169 PPM CACO3 TOTAL NUMBER OF MEASUREMENTS:

290 Limits Exceeded Date/Time 27-Nov-2006 09:00:00 28-Nov-2006 09:15:00 29-Nov-2006 09:36:00 30-Nov-2006 09:15:00 01-Nov-2006 08:45:00 02-Nov-2006 09:02:00 03-Nov-2006 08:40:00 04-Nov-2006 08:21:00 05-Nov-2006 09:38:00 06-Nov-2006 08:45:00 07-Nov-2006 08:55:00 08-Nov-2006 09:25:00 09-Nov-2006 08:15:00.10-Nov-2006 08:52:00 11 -Nov-2006 09:00:00 12-Nov-2006 08:57:00 12-Nov-2006 09:25:00 13-Nov-2006 08:57:00 13-Nov-2006 09:25:00 14-Nov-2006 09:28:00 15-Nov-2006 08:30:00 16-Nov-2006 09:00:00 17-Nov-2006 09:00:00 18-Nov-2006 09:05:00 19-Nov-2006 09:20:00 20-Nov-2006 08:39:00 21-Nov-2006 11:15:00 22-Nov-2006 09:05:00 23-Nov-2006 09:13:00 24-Nov-2006 09:40:00 25-Nov-2006 08:35:00 26-Nov-2006 08:50:00 27-Nov-2006 09:00:00 28-Nov-2006 09:15:00 29-Nov-2006 09:36:00 30-Nov-2006 09:15:00 16-Nov-2006 09:00:00 16-Nov-2006 09:00:00 Power 100.00 100.00 100.00 100.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 5.00 5.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 OpMode 1 I I 5 5 5 5 5 5 4 3'3 1*1 1 1 1 1*1 1 1.1 1 1*I 1 1 1 1 1.1 1 AnalXs TTL CDG CDG TrL TrL TrL ESB ESB TIL MC TTL MC TTL TIZ ESB.ESB ESB ESB TTL JWB TITL TTL NDW NDW 4CDG TSM TSM BLH ESB*MC MLD TrL CDG CDG TrL TrL TrL I END OF MEASUREMENTS REPORT I Repot Geeraed: 2/0/200 11:5 Pge 7of Report Generated:

12/05/2006 11:05 Page 7 of 7 E: 1)OpenCDM Measurements

-Sam plepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 1 I.: Unit: 1 -Wolf Creek Unit 1 2 Sample Point: B-NPDES1 -IST BATCH NPDES TITLE DESCRIPTOR Group- RAD F) Analyis.DONE TSS-1 TSS-Dup TSS %D TRCVD Sysem -BATCH Value/Meas.

Unit I I=Y O=N 0.2 PPM 0.2 ppm 0 1405 TIME Limits Exceeded Datelrime 01-Nov-2006 14:05:00 01-Nov-2006 14:05:00 01-Nov-2006 14:05:00 01-Nov-2006 14:05:00 01-Nov-2006 14:05:00 Power 0.00 0.00 OpMode 5 5 Analyst TLJ TLJ TLJ TLJ TLJ 0.00 5 0.00 5 0.00 5 TOTAL NUMBER OF MEASUREMENTS:

5 I END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 11:04 Page 1 of 1 Report Generated:

12/05/2006 11:04 Page I of I I C;E:OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 4%Unit: 1 -Wolf Creek Unit 1 , Sample Point: THF04A -SEC LIQ WST MON TK A SAnalysis LRP#LRP#LRP#LRP#LRP#COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE Exp Mon Resp Exp Mon Resp Exp Mon Resp Exp Mon Resp Exp Mon Resp MonReadRel MonReadRel MonReadRel MonReadRel MonReadRel H-3 H-3 H-3 H-3 H-3 MN-54 CO-58 CO-58 CO-58 CO-58 CO-58 CO-60 CO-60 CO-60 CO-60 CO-60 SB-125 SB-125 SB-125 SB-125 SB-125 XE-133 TITLE DESCREPTUK Group -RAD System -THF04 Value/Meas.

Unit 2006062 2006070 2006071 2006074 2006077 1 1=Y 0=N SI =Y O=N I I-=Y O=N I I=Y O=N I I=Y O=N 7.13e-05 uCi/ml 6.73e-5 uCi/ml 7.62e-05 uCi/ml 6.21e-05 uCi/ml 6.50e-5 uCi/ml 4.49e-5 uCi/ml 5;68e-05 uCi/ml 4.76e-05 uCi/ml 5.35e-05 uCi/ml 5.39e-05 uCi/ml 1.153e-01 UCI/CC 6.585e-2 UCI/CC 7.431 e-02 UCI/CC 4.940e-02 UCI/CC 5.316e-2 UCI/CC 2.571e-08 UCI/CC 1.086e-05 UCI/CC 1.508e-5 UCI/CC 9.024e-06 UCI/CC 1.158e-05 UCI/CC 1.018e-5 UCI/CC 3.112e-07 UCI/CC 2.169e-7 UCI/CC I.1 17e-07 UCI/CC 2.215e-07 UCI/CC 1.902e-7 UCI/CC 1.126e-06 UCI/CC 3.773e-7 UCI/CC 2.027e-07 UCI/CC 2.977e-07 UCI/CC 4.448e-7 UCIICC 2.127e-07 UCI/CC Limits Exceeded Date/rime 01-Nov-2006 14:05:00 07-Nov-2006 08:05:00 09-Nov-2006 04:00:00 14-Nov-2006 13:50:00 29-Nov-2006 11:20:00 01-Nov-2006 14:05:00 07-Nov-2006 08:05:00 09-Nov-2006 04:00:00 14-Nov-2006 13:50:00 29-Nov-2006 11:20:00 01-Nov-2006 14:05:00 07-Nov-2006 08:05:00 09-Nov-2006 04:00:00 14-Nov-2006 13:50:00 29-Nov-2006 11:20:00 01-Nov-2006 14:05:00 07-Nov-2006 08:05:00 09-Nov-2006 04:00:00 14-Nov-2006 13:50:00 29-Nov-2006 11:20:00 01-Nov-2006 14:05:00 07-Nov-2006 08:05:00 09-Nov-2006 04:00:00 14-Nov-2006 13:50:00 29-Nov-2006 11:20:00 01-Nov-2006 14:05:00 01-Nov-2006 14:05:00 07-Nov-2006 08:05:00 09-Nov-2006 04:00:00 14-Nov-2006 13:50:00 29-Nov-2006 11:20:00 01-Nov-2006 14:05:00 07-Nov-2006 08:05:00 09-Nov-2006 04:00:00 14-Nov-2006 13:50:00 29-Nov-2006 11:20:00 01-Nov-2006 14:05:00 07-Nov-2006 08:05:00 09-Nov-2006 04:00:00 14-Nov-2006 13:50:00 29-Nov-2006 11:20:00 01-Nov-2006 14:05:00 Power 0.00 0.00 0.00 100.00 100.00 0.00 0.00 0.00 100.00 100.00 0.00 0.00 0.00 100.00 100.00 0.00 0.00 0.00 100.00 100.00 0.00 0.00 0.00 100.00 100.00 0.00 0.00 0.00 0.00 100.00 100.00 0.00 0.00 0.00 100.00 100.00 0.00 0.00 0.00 100.00 100.00 0.00 OpMode 5 4 3 1 1 5 4 3 1 1 5 4 3 1 5 4 3 1 I 5 4 3 5 I 5 S 4 3 5 1 3 5 4 3 1 1 5 4 3 1 1 5 Analyst TV ESB AS MC SKW TIJ ESB AS MC SKW TIJ ESB AS MC SKW ESB TLJ TLJ TUJ MDM TLJ ESB AS MC SKW TUJ TLJ ESB AS MC SKW TLJ ESB AS MC SKW TLJ ESB AS MC SKW TLJ Report Generated:

12/05/2006 11:03 Page 1 of2 Report Generated:

12/05/2006 11:03 Page 1 of 2 I OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 1 Unit: 1 -Wolf Creek Unit 1 2 Sample Point: TBF04A -SEC LIQ WST MON TK A TITL Cl C:'6 Gro Syse*E DESCRIPTOR up -RAD m -THF04 Anaib XE-I XE-1 CS-13 CS-13 1-131 1-131 1-131 1-131 1-131 NGT, NGT NGT NGT sig Value/Meas.

Unit Limits Exceeded Date/Time Power OpMode Anal 33 1.021e-06 UCI/CC 14-Nov-2006 13:50:00 100.00 1 35 1.287e-07 UCI/CC 14-Nov-2006 13:50:00 100.00 1 7 6.185e-08 UCI/CC 01-Nov-2006 14:05:00 0.00 5 7 7.744e-08 UCI/CC 14-Nov-2006 13:50:00 100.00 1< 5.537e-08 UCI/CC 01-Nov-2006 14:05:00 0.00 5< 6.726e-8 UCI/CC 07-Nov-2006 08:05:00 0.00 4< 3.728e-08 UCI/CC 09-Nov-2006 04:00:00 0.00 3< 6.488e-08 UCI/CC 14-Nov-2006 13:50:00 100.00 1< 3.530e-8 UCI/CC 29-Nov-2006 11:20:00 100.00 1 S ACT 1.238e-05 UCI/CC 01-Nov-2006 14:05:00 0.00 5 ACT 1.567e-5 UCIICC 07-Nov-2006 08:05:00 0.00 4 ACT 9.338e-06 UCI/CC 09-Nov-2006 04:00:00' 0.00 3 ACT 1.222e-05 UCI/CC 14-Nov-2006 13:50:00 '100.00 1 MC MC TLJ MC TIJ ESB AS MC.KW TLJ ESB AS MC NGT ACT DISS/ENT GAS DISS/ENT GAS DISS/ENT GAS DISS/ENT GAS GRS ACT GRS ACT GRS ACT GRS ACT GRS ACT 1.082e-5 UCI/CC 2.127e-07 uCl/ml 0 uCIl/n 0 uC/ml 1.148e-06 uCI/mi 1.259e-05 UCI/CC 1.567e-5 UCI/CC 9.338e-06 UC1/CC 1.337e-05 UCI/CC 1.082e-5 UCI/CC 29-Nov-2006 11:20:00 01-Nov-2006 14:05:00.07-Nov-2006 08:05:00 09-Nov-2006 04:00:00 14-Nov-2006 13:50:00 01-Nov-2006 14:05:00 07-Nov-2006 08:05:00 09-Nov-2006 04:00:00 14-Nov-2006 13:50:00 29-Nov'-2006 11:20:00 100.00 0.00 0.00 0.00 100.00 0.00 0.00 0.00 100.00 100.00 1 5 4 3 1 5 4 3 1 1 SKW TV ESB AS MC TLJ ESB AS MC SKW TOTAL NUMBER OF MEASUREMENTS:

65 I END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 11:03 Page 2 of 2 Report Generated:

12/05/2006 11:03 Page 2 of 2 I rf,F)OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59:I: Unit: 1 -Wolf Creek Unit 1 2 Sample Poin 9 t: THF04B -SEC LIQ WST MON TK B TrITE DESCRIPTOR Group -RAD System -THF04 F Analvsis LRP#LRP#LRP#LRP#LRP#COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE Exp Mon Resp Exp Mon Resp Exp Mon Resp Exp Mon Resp Exp Mon Resp MonReadRel MonReadRel MonReadRel MonReadRel H-3 H-3 H-3 H-3 H-3 MN-54 MN-54 CO-57 CO-57 CO-58 CO-58 CO-58 CO-58 CO-58 CO-58 FE-59 CO-60 CO-60 CO-60 CO-60 CO-60 CO-60 SB-125 Value/Meas.

Unit 2006069 2006072 2006073 2006075 2006076 1 I=Y =N I I=Y O=N I =Y 0=N I I=Y O=N I =Y O=N 6.18e-05 uCi/ml 6.29E-05 uCi/ml 6.94E-05 uCi/ml 6.49e-05 uCi/ml 5.69e-05 uCi/ml 6.08E-05 uCi/ml 5.08e-05 uCi/ml 5.30e-05 uCi/ml 5.35e-5 uCi/mn 1.228e-01 UCICC 6.565E-02 UCI/CC 6.108E-02 UCI/CC 5.165e-02 UCI/CC 3.909e-02 UCI/CC 2.510e-08 UCI/CC 4.047E-08 UCI/CC 4.814E-08 UCI/CC 4.239E-08 UCI/CC 8.578e-06 UCI/CC 1.664E-05 UCI/CC 1.412E-05 UCIICC 8.714E-06 UCI/CC 9.287e-06 UCI/CC 7.512e-06 UCI/CC 4.758e-08 UCIICC 2.286e-07 UCIICC 2.597E-7 UCI/CC 1.420E-07 UCI/CC 1.515E-07 UCI/CC 2.192e-07 UCI/CC i.843e-07 UCI/CC 6.108e-07 UCI/CC Limits Exceeded Date/Timi 06-Nov-2006 11:30:00 09-Nov-2006 20:00:00 11 -Nov-2006 01:55:00 16-Nov-2006 11:25:00 27-Nov-2006 11:50:00 06-Nov-2006 11:30:00 09-Nov-2006 20:00:00 1 1-Nov-2006 01:55:00 16-Nov-2006 11:25:00 27-Nov-2006 11:50:00 06-Nov-2006 11:30:00 09-Nov-2006 20:00:00 I l-Nov-2006 01:55:00: 16-Nov-2006 11:25:00 27-Nov-2006 11:50:00 09-Nov-2006 20:00:00 1 1-Nov-2006 01:55:00 16-Nov-2006 11:25:00 27-Nov-2006 11:50:00 06-Nov-2006 11:30:00 09-Nov-2006 20:00:00 11-Nov-2006 01:55:00 16-Nov-2006 11:25:00 27-Nov-2006 11:50:00 06-Nov-2006 11:30:00 09-Nov-2006 20:00:00 09-Nov-2006 20:00:00 11-Nov-2006 01:55:00 06-Nov-2006 11:30:00 08-Nov-2006 00:30:00 09-Nov-2006 20:00:00 11-Nov-2006 01:55:00 16-Nov-2006 11:25:00 27-Nov-2006 11:50:00 06-Nov-2006 11:30:00 06-Nov-2006 11:30:00 08-Nov-2006 00:30:00 09-Nov-2006 20:00:00 11-Nov-2006 01:55:00 16-Nov-2006 11:25:00 27-Nov-2006 11:50:00 06-Nov-2006 11:30:00 e Power 0.00 0.00 5.00 100.00 100.00 0.00 0.00 5.00 100.00 100.00.0.00 0.00 5.00 100.00 100.00.'0.00 5.00 100.00 100.00 0.00 0.00 5.00 100.00 100.00 0.00 0.00 0.00 5.00 0.00 0.00 0.00 5.00 100.00 100.00 0.00 0.00 0.00 0.00 5.00 100.00 100.00 0.00 OpMode 5 2 1 1 1 5 2 1 5 2 2 1 1 2 1 5 2 1 5 5 2 2 1 5 4 2 1 1 1 5 5 4 2 5 Analyst TLJ CDG CDG TLJ MC ESB CDG CDG TLJ MC TLJ CDG CDG TLJ MC CDG MDM CDG SKW ESB CDG CDG TLJ MC ESB CDG CDG CDG ESB TSM CDG CDG TLJ MC ESB ESB TSM CDG CDG TLJ MC ESB Report Generated:

12/05/2006 11:03 Page 1~of 2 Report Generated:

12/05/2006 11:03 Page Lof 2 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate E Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1Sample Point: THF04B -SEC LIQ WST MON TK B TITLE DESCRIPTOR Group -RAD System -THF04.Analysi Value/Meas.

Unit Limits Exceeded Date/Time Power OpMode Analyst SB-125 2.513E-7 UCI/CC 08-Nov-2006 00:30:00 0.00 4 TSM SB-125 2.420E-07 UCI/CC 09-Nov-2006 20:00:00 0.00 2 CDG SB-125 2.043E-07 UCLICC 1I -Nov-2006 01:55:00 5.00 1 CDG SB-125 1.763e-07 UCIICC 16-Nov-2006 11:25:00 100.00 1 TLJ SB-125 4.330e-07 UCI/CC 27-Nov-2006 11:50:00 100.00 1 MC XE-133 2.392e-07 UCIICC 06-Nov-2006 11:30:00 0.00 5 ESB XE-133 1.851E-07 UCI/CC 09-Nov-2006 20:00:00 0.00 2 CDG XE-133 4.844e-07 UCIICC 16-Nov-2006 11:25:00 100.00 1 TLJ CS-137 7.984e-08 UCI/CC 27-Nov-2006 11:50:00 100.00 1 MC 1-131 < 6.634e-08 UCI/CC 08-Nov-2006 00:30:00 0.00 4 TSM 1-131 < 4.788E-08 UCI/CC 09-Nov-2006 20:00:00 0.00 2 CDG 1-131 :< 4.993E-08 UCJICC ll-Nov-200601:55:00 5.00 1 CDG 1-131' < 4.233e-08 UCIICC 16-Nov-2006 11:25:00 100.00 1 TLJ 1-131 < 5.11Oe-08 UCI/CC 27-Nov-2006 11:50:00 100.00 1 MC NGT ACT 9.491e-06 UCI/CC 06-Nov-2006 11:30:00 0.00 5 ESB NGT ACT 1.715E-05 UCI/CC 08-Nov-2006 00:30:00 0.00 4 TSM NGT ACT 1.460E-05 UCI/CC 09-Nov-2006 20:00:00 0.00 2 CDG NGT ACT 9.156E-06 UCI/CC 11-Nov-2006 01:55:00 5.00 1 CDG NGT ACT 9.683e-06 UCI/CC 16-Nov-2006 11:25:00 100.00 1 TLJ NGT ACT 8.209e-06 UCI/CC 27-Nov-2006 11:50:00 100.00 1 MC DISS/ENT GAS 2.392e-07 uCI/mi 06-Nov-2006 11:30:00 0.00 5 ESB DISS/ENT GAS 0 uCI/mI 08-Nov-2006 00:30:00 0.00 4 TSM DISS/ENT GAS 1.851E-07 uCI/mI 09-Nov-2006 20:00:00 0.00 2 CDG DISS/ENT GAS 0 uCI/mi 11-Nov-2006 01:55:00 5.00 1 CDG DISS/ENT GAS 4.844e-07 uCI/mI 16-Nov.2006 11:25:00 100.00 1 TLJ DISS/ENT GAS 0 uCI/ml 27-Nov-2006 11:50:00 100.00 1 MC GRS ACT 9.730e-06 UCI/CC 06-Nov-2006 11:30:00 0.00 5 ESB GRS ACT 1.715E-05 UCIICC 08-Nov-2006 00:30:00 0.00 4 TSM GRS ACT 1.478E-05 UCI/CC 09-Nov-2006 20:00:00 0.00 2 CDG GRS ACT 9.156E-06 UCIICC 1 1-Nov-2006 01:55:00 5.00 1 CDG GRS ACT 1.017e-05 UCI/CC 16-Nov-2006 11:25:00 100.00 1 TLJ_GRS ACT 8.209e-06 UCI/CC 27-Nov-2006 11:50:00 100.00 1 MC TOTAL NUMBER OF MEASUREMENTS:

74 1 END OF MEASUREMENTS REPORT -Report Generated:

12/05/2006 11:03 Page 2 of 2 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit:? Sample Point: 9 L'0 E, Analysis COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMWSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE TOTALZR TOTALZR TOTALZR TOTALZR TOTALZR TOTALZR TOTALZR TOTALZR TOTALZR TOTALZR TOTALZR TOTALZR 1 -Wolf Creek Unit 1 BM-D -BLOWDOWN TO LAKE DAILY TITLE DESCRIPTOR Group -RAD System -BM Value/Meas.

Unit 0 I=Y O=N 0 I=Y O=N 0 I=Y O=N SI =Y O=N 0 I=Y O=N 0 I=Y O=N I i=Y O=N I I=Y O=N I I=Y 0=N I I=Y 0=N I I=Y 0=N 1 I-Y 0=N 1 I=Y O=N I I=Y 0=N I I-Y 0=N I I-Y O=N I I=Y O=N I I=Y 0=N I I=Y 0=N I I=Y O=N I I=Y O=N I I=Y O=N I I=Y 0=N I I=Y 0=N I I=Y O=N I I=Y O=N I I=Y 0=N SII-Y 0-N I I-Y 0=N I l=Y O=N 0 GALLONS 29862 GALLONS 0 GALLONS 81350 GALLONS 47275 GALLONS 106130 GALLONS 256570 GALLONS 306675 GALLONS 266095 GALLONS 314725 GALLONS 316250 GALLONS 319440 GALLONS Limits Exceeded Date/Time 01-Nov-2006 11:05:00 02-Nov-2006 14:40:00 03-Nov-2006 15:45:00 04-Nov-2006 22:20:00 05-Nov-2006 15:50:00 06-Nov-2006 08:55:00 07-Nov-2006 12:30:00 08-Nov-2006 10:55:00 09-Nov-2006 08:30:00 10-Nov-2006 08:00:00 1 I-Nov-2006 08:12:00 S12-Nov-2006.08:

10:00 13-Nov-2006 08:10:00 14-Nov-2006 08:15:00 15-Nov-2006 08:00:00 16-Nov-2006 08:00:00 17-Nov-2006 08:10:00 18-Nov-2006 08:00:00 19-Nov-2006 08:05:00 20-Nov-2006 08:00:00 2 1-Nov-2006 08:20:00 22-Nov-2006 08:00:00 23-Nov-2006 08:00:00 24-Nov-2006 08:00:00 25-Nov-2006 08:00:00 26-Nov-2006 09:45:00 27-Nov-2006 08:00:00 28-Nov-2006 08:30:00 29-Nov-2006 08:00:00 30-Nov-2006 08:00:00 04-Nov-2006 22:20:00 05-Nov-2006 11:20:00 07-Nov-2006 12:30:00 08-Nov-2006 10:55:00 09-Nov-2006 08:30:00 10-Nov-2006 08:00:00 I 1-Nov-2006 08:12:00 12-Nov-2006 08:10:00 13-Nov-2006 08:10:00 14-Nov-2006 08:15:00 15-Nov-2006 08:00:00 16-Nov-2006 08:00:00 Power 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 5.00 5.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 0.00 0.00 0.00 0.00 0.00 5.00 5.00 100.00 100.00 100.00 100.00 100.00 OpMode 5 5 5 5 5 5 4 3 3 1 1 1 4 3 3 1 1 1 1 1 1 1 1 1 1 1 I 1 1 1 5 5 4 3 3 1 1 I 1 1 1 1 Analyst TLJ ESB TTL TSM ESB TLJ ESB TLJ ESB ESB ESB TLJ TU TLJ TLJ TLJ SKW NDW NDW CDG ESB ESB TSM ESB MC MLD MC MLD SKW MDM TSM SKW ESB TLJ ESB ESB ESB TLJ TLJ TLJ TLJ TLJ 06-0 Report Generated:

12/05/2006 11:04 Page 1 of2 Report Generated:

12/05/2006 11:04 Page I of 2 E: bi OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1 2 Sample Point: BM-D -BLOWDOWN TO LAKE DAILY TITLE DESCRIPTOR Group -RAD System -BM Analvsis Value/Meas.

Unit Limits Exceeded Date/Fime Power OpMode Ana TOTALZR 323465 GALLONS 17-Nov-2006 08:10:00 100.00 1 S TOTALZR 317660 GALLONS 18-Nov-2006 08:00:00 100.00 1 N TOTALZR 325849 GALLONS 19-Nov-2006 08:05:00 100.00 1 N TOTALZR 324847 GALLONS 20-Nov-2006 08:00:00 100.00 1 (TOTALZR 308350 GALLONS 21-Nov-2006 08:20:00 100.00 1 TOTALZR 265230 GALLONS 22-Nov-2006 08:00:00 100.00 1 TOTALZR 258700 GALLONS 23-Nov-2006 08:00:00 100.00 1 TOTALZR 264300 GALLONS 24-Nov-2006 08:00:00 100.00 1 TOTALZR 264992 GALLONS 25-Nov-2006 08:00:00 100.00 1 TOTALZR 287690 GALLONS 26-Nov-2006 09:45:00 100.00 1 TOTALZR 240302 GALLONS 27-Nov-2006 08:00:00 100.00 1 TOTALZR 225400 GALLONS 28,Nov-2006 08:30:00 100.00 1 TOTALZR 85290 GALLONS 29-Nov-2006'08:00:00 100.00 1 s TOTALZR 250420 GALLONS 30-Nov-2006 08:00:00..

100.00 1 DISS/ENT GAS 0 uCl/ml 04-Nov-2006 22:20:00:

0.00 5__GRS ACT 0 UCI/CC 04-Nov-2006.22:20:00' 0.00 5 TOTAL NUMBER OF MEASUREMENTS:

58 KW TDW rDW ESB ESB[ISM ESB MC 4LD MC V¶LD KW KDM rSM TSM I END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 11:04 Page 2 of 2 Report Generated:

12/05/2006 11:04 Page 2 of 2 I OpenCDM Measurements

-Samplepoint, Analysis, SampledateWolf Creek E: From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1 t: Sample Point: BM-M -BLOWDOWN TO LAKE MONTHLY q TITLE DESCRIPTOR Group -RAD O Syste -BM Analysis Value/Meas.

Unit Limits Exceeded Datefrime Power OpMode Analyst COMPSAVE I =Y 0=N 01-Nov-2006 14:00:00 0.00 5 TLJ ALPHA < 1.346E-08 UCI/CC 01-Nov-2006 14:00:00 0.00 5 NDW_H-3 2.066e-05 UCI/CC 01-Nov-2006 14:00:00 0.00 5 TLJ TOTAL NUMBER OF MEASUREMENTS:

3 1 END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 11:06 Page 1 of 1 Report Generated:

12/05/2006 11:06 Page I of 1 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 1 Unit: 1 -Wolf Creek Unit 1 1.(I)8 Sample Point: WWT -WWT NPDES DISCHARGE TITLE DESCRIPTOR Group- SHOP Systen -WWT Analysis Value/Meas.

Unit Limits Exceeded BA BA BA!O/G O/G O/G O/G O/G O/G O/G O/G.%D 0/61 O/G O/G O/G%R%R%R PH PH PH PH-PH-PH-%D%D%D SO4 Sul UIN;IN Blank Blank Blank Dup.I Il=A,2=B I I1A,2=B I 1=A,2=B< 1.0 ppm< 1.0 ppm< 1.0 ppm 2.2 PPM 1.5 PPM 1.6 PPM 2.7 ppm Date/Time 05-Nov-2006 09:15:00 14-Nov-2006 13:25:00 27-Nov-2006 08:50:00 05-Nov-2006 09:15:00 14-Nov-2006 13:25:00 27-Nov-2006 08:50:00 05-Nov-2006 09:15:00 14-Nov-2006 13:25:00 27-Nov-2006 08:50:00 05-Nov-2006 09:15:00 Power OvMode Analyst 0.00 100.00 100.00 0.00 100.00 100.00 0.00 100.00 100.00 0.00.S 1 1 5 1 1 5 1 1 5 TrL TTL AS TaL TaL AS TrL TTL Dup. 1.5 ppm 27-Nov-2006 08:50:00 100.00 1 TrL-O/G 20.4 % 05-Nov-2006 09:15:00 0.00 5 TTL-O/G 6.5 % 27-Nov-2006 08:50:00 100.00 1 TaM Matrix Spike 33.2 ppm 05-Nov-2006 09:15:00 0.00 5 Ta Matrix Spike 37.5 ppm 14-Nov-2006 13:25:00 100.00 1 AS Matrix Spike 29.9 ppm 27-Nov-2006 08:50:00 100.00 1 TTL-O/G 95.3 % 05-Nov-2006 09:15:00 0.00 5 TaT-O/G 94 % 14-Nov-2006 13:25:00 100.00 1 AS-O/G 88 % 27-Nov-2006 08:50:00 100.00 1 TaL 7.3 PH 05-Nov-2006 09:15:00 0.00 5 TIa 8.0 PH 14-Nov-2006 13:25:00 100.00 1 TaM 8.9 PH 27-Nov-2006 08:50:00 100.00 1 ITa DUP 7.3 PH 05-Nov-2006 09:15:00 0.00 5 TrL DUP 8.1 PH 14-Nov-2006 13:25:00 100.00 1 TaL DUP 8.9 PH 27-Nov-2006 08:50:00 100.00 1 Ta-pH 0 % 05-Nov-2006 09:15:00 0.00 5 TaL-pH 1.2 % 14-Nov-2006 13:25:00 100.00 1 TaT-pH 0% 27-Nov-2006 08:50:00 100.00 1 TTL 4 Blank < 5.0 ppm 05-Nov-2006 09:15:00 0.00 5 TTL FATE 47.6 PPM 05-Nov-2006 09:15:00 0.00 5 Ta S04 Duplicate%D -S04 S04 Matrix Spike%R -S04 TSS-1 TSS-I TSS-1 TSS-Dup TSS-Dup TSS-Dup%D -TSS%D -TSS 47.6 ppm 0%20.1 ppm 100.5 %11.7 PPM 8.8 PPM 7.9 PPM 11.7 ppm 7.9 ppm 8.4 ppm 0%10.8 %05-Nov-2006 09:15:00 05-Nov-2006 09:15:00 05-Nov-2006 09:15:00 05-Nov-2006 09:15:00 05-Nov-2006 09:15:00 14-Nov-2006 13:25:00 27-Nov-2006 08:50:00 05-Nov-2006 09:15:00 14-Nov-2006 13:25:00 27-Nov-2006 08:50:00 05-Nov-2006 09:15:00 14-Nov-2006 13:25:00 0.00 0.00 0.00 0.00 0.00 100.00 100.00 0.00 100.00 100.00 0.00 100.00 5 5 5 5 5 5 1 S 1 1 5 1 TTL TTL TaL TTL TTL TTL TTL TTL TTL Report Generated:

12/05/2006 10:56 Page 1 of2 Report Generated:

12/05/2006 10:56 Page 1 of 2 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1 2 Sample Point: WWT -WWT NPDES DISCHARGE TITLE DESCRIPTOR Group -SHOP System -WWT E- Analysis Value/Meas.

Unit Limits Exceeded Date/fime Power OpMode Analyst%D -TSS 6.1% 27-Nov-2006 08:50:00 100.00 1 IT, FLOW/MGD 0.056 MGD 03-Nov-2006 23:00:00 0.00 5 BLH FLOW/MGD 0.041 MGD 10-Nov-2006 23:00:00 100.00 1 BLH FLOW/MGD 0.037 MGD 17-Nov-2006 23:00:00 100.00 1 BLH ETA Blank < 2.5 ppm 05-Nov-2006 09:15:00 0.00 5 TIL ETA < 2.5 PPM 05-Nov-2006 09:15:00 0.00 5 TTL ETA-DUP < 2.5 ppm 05-Nov-2006 09:15:00 0.00 5 TL%D -ETA 0 % 05-Nov-2006 09:15:00 0.00 5 TTL ETA Matrix Spike 20.4 ppm 05-Nov-2006 09:15:00 0.00 5 TTL_%R -ETA 102 % 05-Nov-2006 09:15:00 0.00 5 " TTL TOTAL NUMBER OF MEASUREMENTS:

52 END OF MEASUREMENTS REPORT.R GeA Report Generated:

12/05/2006 10:56 Page 2 of 2 I E.OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 I.Sample C)Anlyi Unit: 1 -Wolf Creek Unit 1 Point: WWT-D -DAILY COMPOSITE SAMPLE TITLE DESCRIPTOR Group -RAD System -WWT Analsis COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE*COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COMPSAVE COBASAVE BASIN BASIN BASIN BASIN BASIN BASIN BASIN BASIN BASIN BASIN Value/Meas.

Unit 0 I=Y O=N I I=Y O=N 0 I=Y 0=N I I=Y O=N 0 I=Y 0=N I I=Y O=N 0 I=Y O=N I I=Y O=N I I=Y O=N 0 I=Y O=N Limits Exceeded I I=Y O=N 0 1=Y O=N 0 1=Y O=N 0 I=Y O=N I I=Y 0=N 0 I=Y O=N 0 I=Y 0=N I I=Y O=N 0 I=Y O=N 0 1=Y O=N 0 I=Y O=N 0 I=Y O=N 0 I=Y 0=N 0 I=Y O=N 0 I=Y O=N 0 I=Y 0=N 0 1=Y 0=N 0 I=Y O=N I I=Y O=N 0 l=Y O=N I I=Y O=N 0 I=Y O=N I I=A,2=B 2 I=A,2=B I I=A,2=B 2 I=A,2=B I I=A,2=B 2 l=A,2=B I I=A,2=B 2 I=A,2=B I I=A,2=B 2 I=A,2=B Date/Time 01-Nov-2006 10:55:00 01-Nov-2006 20:50:00 02-Nov-2006 08:40:00 03-Nov-2006 10:30:00 04-Nov-2006 08:40:00 05-Nov-2006 09:15:00 06-Nov-2006 09:00:00 07-Nov-2006 15:00:00 08-Nov-2006 21:05:00 09-Nov-2006 18:00:00 10-Nov-2006 01:20:00..

11-Nov-2006 18:15:00 12-Nov-2006.09:50:00.:.

13-Nov-2006 09:50:00 14-Nov-2006 13:25:00 15-Nov-2006 07:40:00 16-Nov-2006 09:25:00 17-Nov-2006 09:55:00 .18-Nov-2006 08:55:00 19-Nov-2006 09:10:00 20-Nov-2006 09:00:00 2 1-Nov-2006 10:45:00 22-Nov-2006 09:25:00 23-Nov-2006 09:25:00 23-Nov-2006 09:30:00 24-Nov-2006 11:30:00 25-Nov-2006 08:30:00 26-Nov-2006 09:00:00 27-Nov-2006 08:50:00 28-Nov-2006 09:30:00 29-Nov-2006 10:15:00 30-Nov-2006 14:00:00 01-Nov-2006 20:50:00 03-Nov-2006 10:30:00 05-Nov-2006 09:15:00 07-Nov-2006 15:00:00 08-Nov-2006 21:05:00 10-Nov-2006 01:20:00 14-Nov-2006 13:25:00 17-Nov-2006 09:55:00 27-Nov-2006 08:50:00 29-Nov-2006 10:15:00 Power 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00*5.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 0.00 0.00 0.00 0.00 0.00 0.00 100.00 100.00 100.00 100.00 OpMode 5 5 5 5 5 5 5 4 3 2 2 1 1 1 1 1 1 1 3 2 1 1 1 1 I 1 1 1 1 1 5 5 5 4 3 2 1 Analyst TrL TSM TTL ESB ESB TrL MC TaL MC MC BLH TrL ESB ESB TTL AS TrL TrL NDW NDW CDG TSM TSM TSM BLH ESB MC MLD TrL CDG TaL TM TSM ESB TTL MC BLH TrL TrL TaL TaL Report Generated:

12/05/2006 10:57 Page 1 of2 Report Generated:

12/05/2006 10:57 Page 1 of 2 I OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Li Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1 ,'. Sample Point: WWT-D -DAILY COMPOSITE SAMPLE TITLE DESCRIPTOR ii Group -RAD O System -WWT 0 SAnalysi Value/Meas.

Unit Limits Exceeded Dateffime Power OpMode Analys DISS/ENT GAS 0 uCl/ml 01-Nov-2006 20:50:00 0.00 5 TSM TOTAL NUMBER OF MEASUREMENTS:

43 1 END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 10:57 Page 2 of 2 Report Generated:

12/05/2006 10:57 Page 2 of 2 c;E: OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1 Sample Point: LAKE -LAKE OVERFLOW TITLE DESCRIPTOR Group -SHOP Systan -LAKE Ci Analys.s FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD Value/Meas.

Unit 0 MGD 0 MGD 0 MGD 0 MGD 0 MGD Limits Exceeded Datetrim e 08-Nov-2006 09:05:00 16-Nov-2006 08:30:00 16-Nov-2006 08:50:00 23-Nov-2006 08:35:00 29-Nov-2006 09:15:00 Power 0.00 100.00 100.00 100.00 100.00 OpMode 3 1 1 1 1 Analyst TrL BLH TrL BLH CDG TOTAL NUMBER OF MEASUREMENTS:

5 1 END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 11:01 Page 1 of 1 Report Generated:

12/05/2006 11:01 Page 1 of 1 OpenCDM Measurements

-Sam plepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1 2 Sample Point: WCCL -WOLF CREEK COOLING LAKE 9 TITLE DESCRIPTOR 2: Group- SHOP 0 Syser -WCCL F Analysis Value/Meas.

Unit Limits Exceeded Date/lTime Power OpMode Analyst LKLEVEL 1084.7 FEET 01-Nov-2006 09:00:00 0.00 5 TmL LKLEVEL 1084.7 FEET 02-Nov-2006 09:20:00 0.00 5 TI'L LKLEVEL 1084.7 FEET 03-Nov-2006 09:25:00 0.00 5 ESB LKLEVEL 1084.7 FEET 04-Nov-2006 07:50:00 0.00 5 ESB LKLEVEL 1084.7 FEET 05-Nov-2006 14:50:00 0.00 5 TTL LKLEVEL 1084.7 FEET 06-Nov-2006 08:10:00 0.00 5 MC LKLEVEL 1084.8 FEET 07-Nov-2006 09:10:00 0.00 4 TTL LKLEVEL 1084.8 FEET 08-Nov-2006 09:05:00 0.00 3 TTL LKLEVEL 1084.8 FEET 09-Nov-2006 08:35:00 0.00 3 MC LKLEVEL 1084.8 FEET 10-Nov-2006 09:07:00 5.00 1 TTL LKLEVEL 1084.9 FEET 11-Nov-2006 09:13:00 5.00 1 TTL LKLEVEL 1086.9 FEET 12-Nov-2006 07:35:00 100.00 1 ESB LKLEVEL 1084.9 FEET 12-Nov-2006 08:20:00 100.00 1 ESB LKLEVEL 1084.9 FEET 13-Nov-2006 07:35:00 100.00 1 ESB LKLEVEL 1084.9 FEET 13-Nov-2006 08:20:00 100.00 1 ESB LKLEVEL 1084.9 FEET 14-Nov-2006 09:43:00 100.00 1 TTL LKLEVEL 1084.9 FEET 15-Nov-2006 07:40:00 100.00 1 AS LKLEVEL 1084.9 FEET 16-Nov-2006 09:25:00 100.00 1 TTL LKLEVEL 1085.0 FEET 17-Nov-2006 09:17:00 100.00 1 TTL LKLEVEL 1084.9 FEET 18-Nov-2006 09:20:00 100.00 1 NDW LKLEVEL 1084.9 FEET 19-Nov-2006 09:30:00 100.00 1 NDW LKLEVEL 1085.0 FEET 20-Nov-2006 08:45:00 100.00 1 CDG LKLEVEL 1085.0 FEET 21-Nov-2006 07:45:00 100.00 1 TSM LKLEVEL 1084.9 FEET 22-Nov-2006 09:10:00 100.00 1 TSM LKLEVEL 1085.0 FEET 23-Nov-2006 08:00:00 100.00 1 BLH LKLEVEL 1085.1 FEET 25-Nov-2006 08:50:00 100.00 1 MC LKLEVEL 1085.1 FEET 26-Nov-2006 09:00:00 100.00 1 MLD LKLEVEL 1085.1 FEET 27-Nov-2006 08:11:00 100.00 1 TTL LKLEVEL 1085.3 FEET 28-Nov-2006 09:30:00 100.00 1 CDG LKLEVEL 1085.3 FEET 29-Nov-2006 09:45:00 100.00 1 CDG LKLEVEL 1085.2 FEET 30-Nov-2006 08:10:00 100.00 1 "TL PH 8.5 PH 08-Nov-2006 09:05:00 0.00 3 TTL CHLORIDE 42 PPM 08-Nov-2006 09:05:00 0.00 3 TTL SULFATE 137 PPM 08-Nov-2006 09:05:00 0.00 3 TTL GRS ACT 0 UCIICC 08-Nov-2006 09:05:00 0.00 3 TITL H-3 1.226e-05 UCI/CC 08-Nov-2006 09:05:00 0.00 3 TrL TOTAL NUMBER OF MEASUREMENTS:

36 END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 11:01 Page 1 of I OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59:1 Unit: 1 -Wolf Creek Unit I I Sample Point: 9 LIME -LIME SLUDGE NPDES TITLE DESCRIPTOR Group -SHOP System -LIME AnalOsis FLOW/MGD FLOW/MGD FLOW/MGD FLOW/MGD FLO W/MGD Value/Meas.

Unit 0 MGD 0 MGD 0 MGD 0 MGD 0 MGD Limits Exceeded Date/rime 02-Nov-2006 08:40:00 06-Nov-2006 08:40:00 16-Nov-2006 08:10:00 23-Nov-2006 09:10:00 29-Nov-2006 08:55:00 Power 0.00 0.00 100.00 100.00 100.00 OpMode 5 5 1 I 1 Analyst TrL MC TTL BLH CDG TOTAL NUMBER OF MEASUREMENTS:

5 I END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 11:02 Page 1 of 1 Report Generated:

12/05/2006 11:02 Page 1 of 1 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1 2 Sample Point: LSP -LIME SLUDGE POND 9 TITLE DESCRIPTOR Group -SHOP System -LSP C, Analysis O/G PH TSS-1 GRS ACT H-3 Value/Meas.

Unit< 1.0 PPM 7.5 PH 6.0 PPM 0 UCI/CC< 1.957e-06 UCI/CC Limits Exceeded DatefTime 08-Nov-2006 08:45:00 08-Nov-2006 08:45:00 08-Nov-2006 08:45:00 08-Nov-2006 08:45:00 08-Nov-2006 08:45:00 Power 0.00 0.00 0.00 0.00 0.00 OpMode Analyst 3 AS 3 TTL 3 TTL 3 TTL 3 TTL TOTAL NUMBER OF MEASUREMENTS:

5 1 END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 11:02 Page 1 of 1 Report Generated:

12/05/2006 11:02 Page I of 1 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 I I-Unit: 1 -Wolf Creek Unit 1 2 Sample Point: SERV -SERVICE WATER CHLORINATION TITLE DESCRIPTOR Group -SHOP SA slSystem -SERV~;Analyis

~ Value/Meas.

Unit Limits Exceeded Datetimin e FAC FAC FAC FAC FAC FAC FAC FAC TRC TRC TRC TRC TRC TRC TRC TRC TRC-TRC-.TRC-TRC-TRC-TRC-TRC-TRC-%D°/o£)-1%/D-'%/D.-%/D-'%/D-'%/D-'%/D-'PH PH PH PH PH PH PH PH" PH-D PH-D 0.01 PPM 0.01 PPM 0.02 PPM 0.02 PPM 05-Nov-2006 13: 10:00 06-Nov-2006 10:55:00 12-Nov-2006 14:00:00 16-Nov-2006 15:05:00 Power 0.00 0.00 100.00 100.00 O5Mode 5 5 1 1 Analyst TTL Mc ESB I'L< 0.01 PPM 22-Nov-2006 11:10:00 100.00 1 TSM< 0.01 PPM 23-Nov-2006 10:30:00 100.00 1 BLH 0.01 PPM 27-Nov-2006 13:15:00 100.00 1 TML 0.02 PPM 30-Nov-2006 11:25:00 100.00 1 TTL 0.13 PPM 05-Nov-2006 13:10:00 0.00 5 TTL 0.13 PPM 06-Nov-2006 10:55:00 0.00 5 Mc 0.13 PPM 12-Nov-2006 14:00:00 100.00 1 ESB 0.16 PPM 16-Nov-2006.15:05:00 100.00 1 TTL< 0.01 PPM 22-Nov-2006

-11::10:00 100.00 1 TSM< 0.01 PPM 23-Nov-2006 10:30:00 100.00 1 BLH 0.08 PPM 27-Nov-2006 13:15:00 100.00 1 TL 0.19 PPM 30-Nov-2006 11:25:00.

100.00 1 TT, DUP 0.13 PPM 05-Nov-2006 13:10:00 0.00 5 1TL DUP 0.13 PPM 06-Nov-2006 10:55:00 0.00 5 Mc DUP 0.13 PPM 12-Nov-2006 14:00:00 100.00 1 ESB DUP 0.16 PPM 16-Nov-2006 15:05:00 100.00 1 TML DUP < 0.01 PPM 22-Nov-2006 11:10:00 100.00 1 TSM DUP < 0.01 PPM 23-Nov-2006 10:30:00 100.00 1 BLH-DUP 0.08 PPM 27-Nov-2006 13:15:00 100.00 1 TTL DUP 0.18 PPM 30-Nov-2006 11:25:00 100.00 1 TTL TRC 0 % 05-Nov-2006 13:10:00 0.00 5 TTL RC 0 % 06-Nov-2006 10:55:00 0.00 5 MC rRC 0 % 12-Nov-2006 14:00:00 100.00 1 ESB rRC 0 % 16-Nov-2006 15:05:00 100.00 1 TTL rRC 0 % 22-Nov-2006 11:10:00 100.00 1 TSM rRC 0 % 23-Nov-2006 10:30:00 100.00 1 BLH rRC rRC)UP)UP 0%5.4 %8.3 PH 8.4 PH 8.5 PH 8.4 PH 8.5 PH 8.5 PH 8.4 PH 8.3 PH 8.3 PH 8.4 PH 27-Nov-2006 13:15:00 30-Nov-2006 11:25:00 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 12-Nov-2006 14:00:00 16-Nov-2006 15:05:00 22-Nov-2006 11:1 0:00 23-Nov-2006 10:30:00 27-Nov-2006 13:15:00 30-Nov-2006 11:25:00 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 100.00 100.00 0.00 0.00 100.00 100.00 100.00 100.00 100.00 100.00 0.00 0.00 5 5 5 1 1 5 5 TIL TrL TrL Mc ESB TIL TSM BLH TTL TrL TTL MC Report Generated:

12/05/2006 11:07 Page 1 of2 Report Generated:

12/05/2006 11:07 Page 1 of 2 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1? Sample Point: SERV -SERVICE WATER CHLORINATION11 IrLi JL.flJlr I U Group- SHOP System -SERV Analysis PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP%D-pH%D -pH Value/Meas.

Unit 8.5 PH 8.4 PH 8.5 PH 8.5 PH 8.4 PH 83 PH 0%0%Limits Exceeded Date/rime 12-Nov-2006 14:00:00 16-Nov-2006 15:05:00 22-Nov-2006 11:10:00 23-Nov-2006 10:30:00 27-Nov-2006 13:15:00 30-Nov-2006 11:25:00 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 Power 100.00 100.00 100.00 100.00 100.00 100.00 0.00 0.00 OpMode I I 1 1 1*1 5 5 Analyst ESB TSM BLH TmL TTL TrL MC%D-0/oD-%D-FLO'pH 0 % 12-Nov-2006 14:00:00 100.00 1 ESB pH 0 % 16-Nov-2006 15:05:00 100.00 1 TrL pH 0 % 22-Nov-2006 11:10:00 100.00 1 TSM pH 0 % 23-Nov-2006 10:30:00' 100.00 1 BLH pH 0 % 27-Nov-2006 13:15:00 100.00 1 TTL pH 0% 30-Nov-2006 11:25:00 100.00 1 TTL W/TGM 39 TGM 05-Nov-2006 13:10:00 0.00 5 TTL FLOW/TGM 37.5 TGM 06-Nov-2006 10:55:00.

0.00 5 MC FLOW/TGM 39 TGM 12-Nov-2006 14:00:00 100.00 1 ESB FLOW/TGM 39 TGM 16-Nov-2006 15:05:00 100.00 1 TTL FLOW/TGM 24.5 TGM 22-Nov-2006 11:10:00 100.00 1 TSM FLOW/TGM 39 TGM 23-Nov-2006 10:30:00 100.00 1 BLH FLOW/TGM 39 TGM 27-Nov-2006 13:15:00 100.00 1 TML FLOW/TGM 22.5 TGM 30-Nov-2006 11:25:00 100.00 1 TITL CUPROSTAT 14.6 PPM 03-Nov-2006 09:15:00 0.00 5 ESB CUPROSTAT 21 PPM 17-Nov-2006 09:45:00 100.00 1 TTL H-130M 4.8 ppm 28-Nov-2006 12:40:00 100.00 1 CDG H-130M < 0.5 ppm 29-Nov-2006 09:50:00 100.00 1 CDG TOTAL NUMBER OF MEASUREMENTS:

68 1 END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 11:07 Page 2 of 2 Report Generated:

12/05/2006 11:07 Page 2 of 2 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59:. Unit: 1 -Wolf Creek Unit 1 , Sample Point: UHS A -ESWA/OUTFALL 006 9 (*1 (1 TITLE DESCRIPTOR Group- SHOP System -SERV Analysis DIVERSN DIVERSN DIVERSN DIVERSN DIVERSN DIVERSN DIVERSN DIVERSN FLOW/TGM FLOW/TGM FLOW/TGM FLOW/TGM FLOW/TGM FLOW/TGM FLOW/TGM FLOW/TGM PH PH PH PH PH PH PH PH PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP%D -pH%D -pH%D-pH%D-pH%D -pH%D -pH%D -pH%D- pH TRC TRC Value/Meas.

Unit I I=Y O=N I I=Y 0=N I I=Y 0=N I I=Y 0=N I I=Y O=N I I=Y O=N SI =Y 0=N 0 I=Y O=N 28.1 TGM 27.0 TGM 28 TGM 28.1 TGM" 17.7 TGM 28.1 TGM 28.1 TGM 16.2 TGM 8.3 PH 8.4 PH 8.5. PH 8.4 PH 8.5 PH 8.5 PH 8.4 PH 8.3 PH 8.3 PH 8.4 PH 8.5 PH 8.4 PH 8.5 PH 8.5 PH 8.4 PH 8.3 PH 0%0%0%0%0%0%0%0%0.13 PPM 0.13 PPM Limits Exceeded Date/rime 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 12-Nov-2006 14:00:00 16-Nov-2006 15:05:00 22-Nov-2006 11: 10:00 23-Nov-2006 10:30:00 27-Nov-2006 13:15:00 30-Nov-2006 11:20:00 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 12-Nov-2006 14:00:00 16-Nov-2006.15:05:00 22-Nov-2006.11:10:00.

23-Nov-2006 10:30:00 27-Nov-2006 13:15:00 30-Nov-2006 11:20:00 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 12-Nov-2006 14:00:00 16-Nov-2006 15:05:00 22-Nov-2006 11:10:00 23-Nov-2006 10:30:00 27-Nov-2006 13:15:00 30-Nov-2006 11:20:00 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 12-Nov-2006 14:00:00 16-Nov-2006 15:05:00 22-Nov-2006 11:10:00 23-Nov-2006 10:30:00 27-Nov-2006 13:15:00 30-Nov-2006 11:20:00 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 12-Nov-2006 14:00:00 16-Nov-2006 15:05:00 22-Nov-2006 11: 10:00 23-Nov-2006 10:30:00 27-Nov-2006 13:15:00 30-Nov-2006 11:20:00 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 Power 0.00 0.00 100.00 100.00 100.00 100.00 100.00 100.00 0.00 0.00 100.00 100.00 100.00 100.00 100.00 100.00 0.00 0.00 100.00 100.00 100.00 100.00.100.00 100.00 0.00 0.00 100.00 100.00 100.00 100.00 100.00 100.00 0.00 0.00 100.00 100.00 100.00 100.00 100.00 100.00 0.00 0.00 OpMode 5 5 1 1 1 1 1 1 5 5 5 5 1 1 I 1 S1 1 5 1 1 1*I 5 1 1 1 1 1 I 5 5 AnaVst TrL MC ESB TIL TSM BLH TIL TaL TTL MC ESB TTL TSM BLH TIa TaL TaL MC ESB TaL TSM BLH TaL TaL TaL MC ESB TIL TSM BLH TL MC ESB TfL TSM BLH TTL TrL TaL MC Page 1 of 2 Report Generated:

12/05/2006 11:08 Repot Gneraed:12/5/206 1:08Page 1 of 2 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1 2 Sample Point: UHS A -ESWA/OUTFALL 006 TITLE DESCRIPTOR Group -SHOP 0i System -SERV E; Analysis Value/Meas.

Unit Limits Exceeded Date/Time Power OpMode Analys TRC 0.13 PPM 12-Nov-2006 14:00:00 100.00 1 ESB TRC 0.16 PPM 16-Nov-2006 15:05:00 100.00 1 TTL TRC < 0.01 PPM 22-Nov-2006 11:10:00 100.00 1 TSM TRC < 0.01 PPM 23-Nov-2006 10:30:00 100.00 1 BLH TRC 0.08 PPM 27-Nov-2006 13:15:00 100.00 1 MTL TRC < 0.01 PPM 30-Nov-2006 11:20:00 100.00 1 TTL TRC-DUP 0.13 PPM 05-Nov-2006 13:10:00 0.00 5 TIL TRC-DUP 0.13 PPM 06-Nov-2006 10:55:00 0.00 5 MC TRC-DUP 0.13 PPM 12-Nov-2006 14:00:00 100.00 1 ESB TRC-DUP 0.16 PPM 16-Nov-2006 15:05:00 100.00 1 TTL TRC-DUP < 0.01 PPM 22-Nov-2006 11:10:00-

.100.00 1 TSM TRC-DUP < 0.01 PPM 23-Nov-2006 10:30:.00'i

-:100.00 1 BLH TRC-DUP 0.08 PPM 27-Nov-2006 13:15:00.

00.00 1 TTL TRC-DUP < 0.01 PPM 30-Nov-2006 11:20:00, 100.00 1 TTL*%D-TRC 0 % 05-Nov-2006 13:10:00, *0.00 5 TrL%D-TRC: 0 % 06-Nov-2006 10:55:00 .0.00 5 MC*%ID-TRC 0 % 12-Nov-2006 14:00:00 .100.00 1 ESB%/oD-TRC 0 % 16-Nov-2006 15:05:00 .100.00 1 TTL%D-TRC 0 % 22-Nov-2006 11: 10:00 100.00 1 TSM%D-TRC 0 % 23-Nov-2006 10:30:00 100.00 1 BLH%D-TRC 0 % 27-Nov-2006 13:15:00 100.00 1 TTL%D-TRC 0 % 30-Nov-2006 11:20:00 100.00 1 -TIL TOTAL NUMBER OF MEASUREMENTS:

64 END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 11:08 Page 2 of 2 Report Generated:

12/05/2006 11:08 Page 2 of 2 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 I+++Unit: I -Wolf Creek Unit 1 2 Sample Point: UHS B -ESWB/OUTFALL 006 TITLE DESCRIPTOR Group -SHOP System -SERV U Anal DIV DIV DIV DIV DIV DIV DIV DIV FLC FLC FLC FLC FLO FLC FLC PLH PH PH PH PH PH PH PH PH PH-VMSN ERSN ERSN ERSN ERSN ERSN ERSN ERSN ERSN)W/TGM)WITGM Value/Meas.

Unit SI =Y O=N I I =Y O=N 1 I=Y 0=N SI =Y O=N 0 I=Y O=N I I=Y 0=N SI =Y O=N I i=Y O=N 28.1 TGM 27.0 TGM Limits Exceeded Datefrime 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 12-Nov-2006 14:00:00 16-Nov-2006 15:05:00 22-Nov-2006 11:20:00 23-Nov-2006 10:30:00 27-Nov-2006 13:15:00 30-Nov-2006 11:25:00 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 Power OpMode 0.00 5 0.00 5 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 0.00 5 0.00 5 Anal*1)W/TGM. 28 TGM 12-Nov-2006 14:00:00 100.00 1)W/TGM 28.1 TGM 16-Nov.-2006 15:05:00 '100.00 1)W/TGM 17.7 TGM 22-Nov-2006 11:20:00 100.00 1)W/TGM 28.1 TGM 23-Nov-2006 10:30:00 100.00 1)W/TGM 28.1 TGM 27-Nov-2006 13:15:00 100.00 1)W/TGM 16.2 TGM 30-Nov-2006 11:25:00 100.00 1 8.3 PH 05-Nov-2006 13:10:00 0.00 5 8.4 PH 06-Nov-2006 10:55:00 0.00 5 8.5 PH 12-Nov-2006 14:00:00 100.00 1 8.4 PH 16-Nov-2006 15:05:00 100.00 1 8.5 PH 22-Nov-2006 11:20:00 100.00 1 8.5 PH 23-Nov-2006 10:30:00 100.00 1 8.4 PH 27-Nov-2006 13:15:00 100.00 1 8.3 PH 30-Nov-2006 11:25:00 100.00 1 DUP 8.3 PH 05-Nov-2006 13:10:00 0.00 5 lvst TIL MC ESB TrL[ISM BLH TTL rrL TrL MC ESB[SM BLH TIL TTL TTL MC ESB TTIL rSM BLH TTL TrL TM MC ESB TaL TSM BLH TrL TTL TaL MC ESB TTL TSM BLH TaL TIM TrL MC PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP PH-DUP%D -pH%D -pH%D -pH%D -pH%D -pH%D -pH%D -pH%D-pH TRC TRC 8.4 PH 8.5 PH 8.4 PH 8.5 PH 8.5 PH 8.4 PH 8.3 PH 0%0%0%0%0%0%0%0%0.13 PPM 0.13 PPM 06-Nov-2006 10:55:00 12-Nov-2006 14:00:00 16-Nov-2006 15:05:00 22-Nov-2006 11:20:00 23-Nov-2006 10:30:00 27-Nov-2006 13:15:00 30-Nov-2006 11:25:00 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 12-Nov-2006 14:00:00 16-Nov-2006 15:05:00 22-Nov-2006 11:20:00 23-Nov-2006 10:30:00 27-Nov-2006 13:15:00 30-Nov-2006 11:25:00 05-Nov-2006 13:10:00 06-Nov-2006 10:55:00 0.00 5 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 0.00 5 0.00 5 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 100.00 1 0.00 5 0.00 5 Report Generated:

12/05/2006 11:08 Page 1 of2 Report Generated:

12/05/2006 11:08 Page I of 2 OpenCDM Measurements

-Samplepoint, Analysis, Sampledate Wolf Creek From: 01-Nov-2006 00:00 To: 30-Nov-2006 23:59 Unit: 1 -Wolf Creek Unit 1 2 Sample Point: UHS B -ESWB/OUTFALL 006 (9 TITLE DESCRIPTOR Group -SHOP System -SERV Analysi Value/Meas.

Unit Limits Exceeded Date/Time Power OpMode Analyst TRC 0.13 PPM 12-Nov-2006 14:00:00 100.00 1 ESB TRC 0.16 PPM 16-Nov-2006 15:05:00 100.00 1 TTL TRC < 0.01 PPM 22-Nov-2006 11:20:00 100.00 1 TSM TRC < 0.01 PPM 23-Nov-2006 10:30:00 100.00 1 BLH TRC 0.08 PPM 27-Nov-2006 13:15:00 100.00 1 TL.TRC 0.19 PPM 30-Nov-2006 11:25:00 100.00 1 MTL TRC-DUP 0.13 PPM 05-Nov-2006 13:10:00 0.00 5 TTL TRC-DUP 0.13 PPM 06-Nov-2006 10:55:00 0.00 5 MC TRC-DUP 0.13 PPM 12-Nov-2006 14:00:00 100.00 1 ESB TRC-DUP 0.16 PPM 16-Nov-2006 15:05:00 100.00 1 .ITL TRC-DUP < 0.01 PPM 22-Nov-2006 11:20:00 100.00 1 TSM TRC-DUP < 0.01 PPM 23-Nov-2006 10:30:00 100.00 1 BLH TRC-DUP '0.08 PPM 27-Nov-2006 13:15:00 100.00 1 'ITL TRC-DUP 0.18 PPM 30-Nov-2006 11:25:00 100.00 1 TTL%D-TRC 0.% 05-Nov-2006 13:10:00 0.00 5 TTL%D-TRC 0 % 06-Nov-2006 10:55:00 0.00 5 MC%D-TRC .0 % 12-Nov-2006 14:00:00 100.00 1 ESB%D-TRC 0 % 16-Nov-2006 15:05:00 100.00 .1 TML%D-TRC 0 % 22-Nov-2006 11:20:00 100.00 1 TSM%D-TRC 0 % 23-Nov-2006 10:30:00 100.00 1 BLH%/0 D-TRC 0 % 27-Nov-2006 13:15:00 100.00 1 TTL%D-TRC 5.4 % 30-Nov-2006 11:25:00 100.00 1 'ITL TOTAL NUMBER OF MEASUREMENTS:

64 1 END OF MEASUREMENTS REPORT Report Generated:

12/05/2006 11:08 Page 2 of 2 Report Generated:

12/05/2006 11:08 Page 2 of 2 Item 3 of Enclosure 4 to Letter ET 07-0001 Analytical Results from Accredited Environmental Laboratory Eonti-nenital fAnlBIticaI Ser/Ices, nc 06/28/2005 9 1:1 C)Wolf Creek Nuclear Operating Co.Attn: Ralph Logsdon P.O. Box 411 Burlington, KS 66839 Date Received:

06/14/2005 Continental File No.: 5796 continental Order No.: 12811 P.O./Project No: 0701240 Purchase Auth: 701240/7

Dear Mr. Logsdon:

This laboratory report consisting of a pages contains for the following samples: the analytical results CAS LAB ID #05060685 SAMPLE DESCRIPTION 003 SAMPLE TYPE Liquid DATE SAMPLED 6/13/2005 Continental is accredited by the State of Kansas through the National Environmental Laboratory Accreditation Program (NELAP). The results contained in this report were obtained using Continental's Standard Operating Procedures.

These procedures are in substantial compliance with the approved methods referenced and the standards published by NELAP.The Appendix and Quality Control sections are an integral part of this report and may contain data qualifiers.

All results are reported on a wet weight basis unless otherwise stated.Samples will be retained for thirty days unless Continental is otherwise notified.Thank you for choosing Continental for this project. If you have any questions please contact me at (800)535-3076.

CONTINENTAL ANALYTICAL SERVICES, INC.Clifford J..er Technical Manager Page: 1 Brian T. O'Donnell Project Manager P.O. BOX 3737 -52S 5. Eighth St. -Salina, KS 67402-3737 785-827-1273 800-53S-3076 Pax 789-623-7830 M2E Znvironmntal Laboratozy Accreditation No. 8-10146

..,.Continental "1;; Andlytical -Services.

Inc..Page: 2 A 9'Client: Wolf Creek Nuclear Operating Co.Attn: Ralph Logsdon P.O. Box 411 Burlington, KS 66839 Date Sample Rptd: 06/28/2005 Date Sample Recd: 06/14/2005 Continental File No: 5796 Continental Order No: 12811 Lab Number: 05060685 Sample

Description:

003 Date Sampled: 06/13/2005 Time Sampled: 0920 Analysis Antimony, Total Arsenic, Total Beryllium, Total Cadmium, Total (GFAA)Chromium, Total Copper, Total Hardness (Calculated)

Lead, Total Mercury, Total Nickel, Total Selenium, Total Silver, Total (GFAA)Thallium, Total Zinc, Total Ammonia, Total, as N Chloride Nitrate, as N pH Analysis Antimony, Total Arsenic, Total Beryllium, Total Cadmium, Total (GFAA)Chromium, Total Copper, Total Hardness (Calculated)

Lead, Total Mercury, Total Nickel, Total Selenium, Total Silver, Total (GPAA)Thallium, Total Zinc, Total Concentration ND(0.006)ND(0.005)ND (0. 004)ND(0.001)ND(0 .010)ND(0.020)217.ND(0.003)NOD(0.0002)

ND(0.040)ND(0.005)ND(0.002)ND(0.002)ND(0.020)ND(0.10)33.7 ..ND(0.1) QC 8.49 H Units mg/L mg/L mg/L mg/L mg/L mg/L mg/L as CaC03 mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L Std. units Book/Page 5983/25 5983/23 5979/26 5983/31 5979/26 5979/26 5979/26 5983/27 5672/255 5979/26 5983/22 5983/29 5983/32 5979/26 5892/277 5966/45 5966/43 5936/48 Date Prepared 06/20/2005 06/20/2005 06/16/2005 06/20/2005 06/16/2005 06/16/2005 06/16/2005 06/20/2005 06/20/2005 06/16/2005 06/20/2005 06/20/2005 06/20/2005 06/16/2005 Date Analyzed 06/22/2005 06/20/2005 06/17/2005 06/23/2005 06/17/2005 06/17/2005 06/17/2005 06/22/2005 06/21/2005 06/17/2005 06/20/2005 06/23/2005 06/23/2005 06/17/2005 QC Inst.Batch Batch 050620-2 101173 050620-1 IGF2171 050616-6 IOIP2167 050620-3 IGF1174 050616-6 10IP2167 050616-6 IOIP2167 050616-7 121P2167 050620-3 G1I173 050620-4 5MA1172 050616-6 IOXP2167 050620-1 IGF2171 050620-3 G11174 050620-3 G1I174 050616-6 1OIP2167 Analyst Method(s)KAM 7041 MAG 7060A KMW 6010B KAM 7131A 1MW 6010B KMW 60103 10W 6010 & SM 2340B KAM CRD 1MW MAO KAM KAM 1MW 7421 7470A 6010B 7740 7761 7841 6010B-Continued-P.0. Box 3737 -525 N. °ighth St. -&LnAa. KS 67402-3737 795-827-1273 800-535-3076 Fax 785-823-7830 a& ,Cp tinental KDHB Enviromental Laboratory Accrediftation No. 8-10146 ,rAnnagtlc ra Sarvlcx. Inc.

G E: b t:]C)Client: Wolf Creek Nuclear Operating Co.Attn: Ralph Logadon P.O. Box 411 Burlington, KS 66839 Page: 3 Date Sample Rptd: 06/28/2005 Date Sample Recd: 06/14/2005 Continental File NO: 5796 Continental Order No: 12811 Date Date Analysis Prepared Analyzed Ammonia, Total, as N N/A 06/17/2005 Chloride N/A 06/16/2005 Nitrate, as N N/A 06/14/2005 pH N/A 06/14/2005 Furnace Metals Total Preparation Method ICP Metals Total Preparation Method Mercury Total Preparation Method Total As & Be by GFAA Preparation Method Antimony Total Preparation Method Calculated as Hardness Preparation Method QC Batch 050617-2 050616-1 050614-1 050614-1 lnst.Batch 050617-4 050616-1 050614-3 050614-1 Analyst Method(s)MLL SM 4500-NH3 (H)JDL 300.0 JDL 300.0 BRE 9040B Metals/3020A 200.7/3010A SM 3112B/7470A 206.2/270.2/7060/774 200.7/3005A 200.7/6010B Conclusion of Lab Number: 05060685 ND(), where noted, indicates none detected with parentheses.

the reporting limit in P.O. BOX 3737 -525 N. Eighth St. -Salina, KS 67402.373737~

783-827-1273 800-533-3076 Fax 85-823-7930 2N3 Rnronmantal Laboratory Accreditation No. B-10146 A.1nUEIcl SuIvcm. Itr.

I'E b-Continental A q 12]Client: Wolf Creek Nuclear Operating Co.Attn: Ralph Logsdon P.O. Box 411 Burlington, KS 66839 Page: 4 Date Sample Rptd: 06/28/2005 Date Sample Recd: 06/14/2005 Continental File No: 5796 Continental Order No: 12811 QC -QC data qualifiers were noted. See the attached QC report.H -Regulatory analytical holding time for this analysis was exceeded.The following table presents the date and time sampled, the date and time analyzed, and the total time elapsed for each analysis with an EPA recomnended holding time of forty-eight hours or less DATE/TIME DATE/TIME ELAPSED CAS LAB ID # ANALYSIS SAMPLED ANALYZED HRB:MIN 05060685 Nitrate, as N 06/13/2005 0920 06/14/2005 1741 32:21 05060685 pH 06/13/2005.

0920 06/14/2005 1415 28:55 All samples were not received at the recommended temperature of less than 6 degrees Celsius.-Conclusion of Laboratory Report-P.O. Soz 3731 -S2S N. Righth St. -Salina, K9 70233 785-827-1273 800-S3S-3076 Fax 785-823-7830 Contine ntal KDME Environmental Laboratory Accreditation No. 3-10146 Ar~l TWAI Vic. Irc.

Analytical -ServIoe5.rinc.

Quality control Report Batch Summary Clients Wolf Creek Nuclear Operating CO.Attn: Ralph L.aftcm P.O. Sox 411 Burlington, ME 66839 Page: 5 Date Reported:

06/28/2005 Date Sample Received:

06/14/2005 Cont4iantal file No% 5796 Continental Order No: 12811 Tent Test-aae OC Batch Method Blank LCS WS Lab No.11.304 Beryllium, Total 050616-6 0506168116 050616LC86 050606SMS 51.308 Chromium, Total 0506I6-6 050616316 05066]LC86 05060585M 81.313 Copper, Total 050616-6 05061631.16 050616LC86 050606851M 8,336 Nickel, Total 050616-6 05061631.16 OS0616LC86 05060665MB 8L369 Zinc. Total 050616-6 050616BL16 050616LC86 05060685MS Lab numbers associated with this batch: 05060685 81.323 Hardness (Calculated) 050616-7 0506163117 050616LC87 05060224K8 Lab numbers associated with this batch: 05060685 81,302 Arsenic, Total 050620-1 050620BLK 0S0620LCOI 81350 Selenium, Total 050620-1 050620LCSI 05061011148 Lab associated with this batch: 05060685 8.01 Antimony.

Total 050620-2 0506201.1 0505201SCS2 05061011148 Lab snmbere ausociated with this batch: 05060685 81.506 Cadmium, Total (OFAA) 050620-3 0506203IJ.3 050620LC83 0506101148 81,328 Lead, Total 050620-3 05062031.13 050620LC83 0506101148 81371 Silver, Total (OWAA) 050620-3 05062031.13 050620LC83 8L360 Thallium, Total 050620-3 0506203L!.3 0S0620L=3 0506101148 Lab numbers aesociated with this batch: 05060685 8L333 Mercury, Total 050620-4 05062031.14 05060840MK Lab numbers associated with thin batch: 05060665 01.120 Axiia, Total, an N 050617-2 050617B312 050617LC82 05060682N8 Lab numbers ansociated with this batch: 05060685 81.502 Chloride 050616-1 05061631.11 050616LCGI 050605508M Lab numbers associated with this batch: 05060685 01.505 Nitrate, as N 050614-1 05061431.11 050614LCI81 05060551MS Lab nubers assuociated with this batch: 05060685-Quality Control Report Continued

-P.O. Box 3737 -525 N. Eighth St. -Salina, ,, 67402-3737 nttl 785-827-1273 800-535-3076 Fax 78S-823-7830 E____________nvironmentl Laxoratory Accreditation No. 8-10146 ArIUUCl Seivicus.

Inc.

441- Continentat

ýý,Anaiuvcai ser-vjces,.:InC4; i i'Pt Clients Wolf Creek Nuclear Operating Co.Attn: Ralph Logadon P.O. Box 411 Bu.rlington., K 66839 Quality Control Report Batch Sumary Page s 6 Date Reported, 06/28/2005 Date Sample Received:

06/14/2005 Continental File no: 5796 Continental Order No: 12811 Tent Totname QC Batch Method Blank LCS M Lab No.GL211 PH 050614-1 050614LCS1 05060685MSB Lab nuabere associated with this batch: 0S060685 Quality Control Report Continued

-P.O. BOX 3737 -S25 N. Eighth St. -Salina,, I 67402-3737-

,__n tinen tal 785-827-1273 800-535-3076 Fax 785-823-7830 EDHE Environmental Laboratory Accreditation Ho. 8-10146 An ai ' 1r4cm.. Inc.

G E: Quality Control Report Method Blank, LCS, 14S/14D Data Clients Wolf Creek Nuclear Operating Co.Attn: Ralph LogAon P.O. SM 411 Burlington, RB 66839 Page: 7 Date Reported:

06/28/2005 Date Sample Received, 06/14/2005 Cotinental vile Not 5796 Continental Order Not 12811 Spiked sample Spiked Sample Blank 11 Roea spike (.

spike preaision Data Amualy1i.

Data LC8 Limit. Level Mnits SI UMD Limit. ZLel tUn RMD zeL" t 0C matcht 050614-1 For smote analysed see 06/14/2008 spiked serie 05060531 Mtirate, " a VD(0.1) 96.1 89.2-107 2.0 ng/I. 0M U0 76.5-121 200 09/1 *' 4.9 0C Ratche 050814-1 lox Savoie amalysed me 06/14/2005 Spiked sample, 50360685 in X/a 7.00 6.90-7.10 7.0 8t. u 8.49 T 8.49 T 0 8td. on 0.0 0.4 OC Mat.he 050414-1 lor ample maalysmed ct 06/16/2005 Spiked ample. 0s060550 enOziw" WD(1.0) 97.7 90.2-104 4.0 19/L INt M 66.0-118 400 Ig/L ** 5.1 OC miste 050616-6 lox samp1le prpared an% 06/16/2005 spiked sample 0506066S5 Dezyllim.

Total MD(0.004) 100 67.3-105 0.5 mg/L 99.9 97.6 84.7-105 0.5 OV/ 2.3 4.3 chz.-LI Total M*(0.010) o01 88.1-109 0.5 mg/i. 99.0 97.7 85.4-109 0.5 ig/L 1.3 7.4.Total s11(0.0201 103 87.5-110 0.5 mg/L 102 101 87.0-114 0.5 mg/L 1.0 8.5 Rik",l Total 10(0.040) 101 90.6-105 0.5 1g/L 98.8 96.6 87.2-106 0.5 1g/A 2.3 4.0 ailso, (0.020) 99.1 87.7-105 0.5 m./L 95.9 95.2 82.3-111 0.5 WG/L 0.7 9.7 00 Ratch. 050616-7 For smples prepared mut 06/16/2005 Spiked sm~ples 05060334 Uardsawi (Caloslatad) 3M(5.0) 101 88.4-110 337 1g/i a MN MIN 15.1-111 337 mg/i*L me 9.4 GO Metch, 050417-2 lor smple analysed see 06/17/2005 Spiked smples 05060682 ain"aI Total., -I 1)(0.10) 104. 92.6-112 1.0 mG/L UM -81.8-116 1.0 mg/i *e 3.9 GO Retch, 050620-1 los siaules Preped m 06/20/2005 Spike samples Axranlo. Totl 3D(0.005) 104 83.1-114 0.05 11/L US Its CC matah. 050620-1 or amplea pepad onm 06/20/2005 Spiked amples 05061011 Elmissin, Total H0(0.005) 101 84.7-112 0.05 mg/i M UK 54.9-118 0.05 mg/i ** 6.2 ac BRtch$ 0506240-2 lor 9l0a prepared m 06/2011205 Spiked 05061011 Total 0(0.006) 102 80.3-113 0.05 319/L IN 90N 76.1-113 0.05 mg/L *4 6.8 CC Reatbh 050620-3 or samlea prepared cu 09/20/2005 Spiked &"VLSTot"l [or".) W0(0.002) 90.0 85.4-113 0.01 ma/3 UN *tw 9C 050620-3 90C Samplsm prepared an* 04/20/2005 Spiked amples 05061011 Ceti=, Total (MML) 311(0.001) 106 63.4-119 0.0025 mg/L as M 68.7-134 0.0025 g/L 29A.6 Lead. Total 30(0.003) 109 86.9-112 0.0S 1g/1, 13 ME 53.5-128 0.05 mg/L 0* 6.6 I'Jall D, Total 30(0.002) 102 89.3-112 0.025 mg/L X11 No 57.2-130 0.025 mg/L ** 9.1 CC Misetc 050620-4 o samples preparsd mn 06/20/3005 Spiked sample 05060840 mL'zy. total 30(0.0002) 105 87.9-111 0.005 m/L 112 101 74.4-121 0.005 mg/i ** 8.8 Dsat Qualifteamx

= -The1111/U1 sample awaYLyee sez not perVoruga Ia a sample frm thia Cmtinant&l order ner.N/a -Not Aplicable T -UKSEDO cant be for tlis snaiyaLs.

Value ShosI 10 the result ot & dtp.lcatJe 021.ly11.

of t1e aamle.S- Limits not available.

R- RI Camnt be CalCUlated.

-The 11/3SD aea1pe analyses swre performed on ths sample from this Continmmtal ordesr mmbr.

aitety control Deport-P.O. Box 3737 -525 N. Eighth St. -Salina, KB 67402-3737 75-827-1273 800-535-3076 Faa 785-823-7830 pntin en t al HE ZUfrUn=an=tal Laboratory ACcLredtatiOn No. 3-10146 s Ams.Inc.

IL r,.1 El[)9 Fii 0 Quality Control Report Continsig Calilbration Verification Data Smry Clients Wolf Creek Nuclear Operating Co.Attne Ralph Logsdon P.O. Doz 411 Burlington., K 66939 Page: 8 Date Reported:

06/29/2005 Date Sample Receiveds 06/14/2005 Continental File Not 5796 Continental Order Not 12811 Analysis Ammoia. Total, pH chloride Nitrate, of N Analysis Antimony Arsenic Beryllium Chromium copper Lead, Totl Nercury, Total Nickel Selenium, Total zinc silver CdI'toum Date of Instrument Amount in A t Analysis Batch ID Standard Detected units as N 06/17/2005 No data qualifiers present for thiL analysis.06/14/2005 No data qualifiers present for this analysis.06/16/2005 No data qualifiers present for this analysis.06/14/2005 050614-4 2.00 1.79 mg/L Samples assciated with this ContinAng Calibration Verifications Laboratory Number Instrument Batch Sample Description 05060685 050614-3 003 percent 99.5 CL Percent Recover.Date of Analysis 06/22/2005 06/20/2005 06/17/2005 06/17/2005 06/17/2005 06/22/2005 06/21/2005 06/17/2005 06/20/2005 06/17/2005 06/23/2005 06/23/2005 Instrunt Amount in Amount Batch XD Standard -Detected unite No data qualifiers present for this analysis.So data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for ts analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.Data Qualifiers:

CL -The Continu4i4 calibration verification CCV) standard recovery for this snalyte wao below the method or SOP limit. The reported sample concentration may be biased low.-Quality Control Report Conclusion-P.O. Box 3737 -525 N. ,ighth St. Salina, IS 67402-3737 785-827-1273 800-535-3076 Pax 785-823-7830 Laboratory Accreditation No. B-10146 .. E olnrtin eOMntl. -In.

COOLER / SAMPLE RECEIPT FORM (C/S RF)Client Name: , y) Q Sample ID: 4=0a File No.: < 9L(Order NO.: A~i Date/Time cool eivcqed: 61O-jJ g&fSý .8B 1 1 /A Unpacked By: q Date entered into LIMS: Red Warning Sticker Applied: Yes By: Date: /-/Samples Screened with Geiger Counter i & No II Date: I I II Cooler Identification:

Cooler Size: Delivered By: Custody Seal: Type of Packing Material: Cooler Temperature

(°C): CAS Cooler #:O ( C. /Client's Cooler/Box/Letter/Hand Delivered Other Small / e Large / NA Uf e X/DHL/ASAP/Land Air Exp/Field Svcs/Mail/Walk-In/Other_

Air Bill Number: (j~~ji tact~ Broken) Absent Seal No: G RAD_________

Seal Name: 4kSeal Date: Seal matches Chain of Custody: Yes / No /@Blue Ic4Dýý am/Paper/Peanuts

/Vermiculite/

NA Original Reading. i- C Corrected Reading ..~ L C Temp. By: Temp. Blank Poured _ Surface G P M Thermo. ID No.: Thermo. Correction Factor (C): es: No r= Yes (See detail below)sent E Sample excluded from Chain of Custody: from: 3 Broken or Leaking Containers:

ter received with samples 0 Sample listed on Chain of Custody, not received: 0 Sample description on container label different hfom ete Chain of Custodyr.saing time sampled El Air bubbles inVOA vials: Sample Receipt Discrepanci 3 Chain of Oastody not pres-f Infrmationobtained Purchase Order/Let E3 Container label absent 13 of Custody incompi 3 Chain of Custody mi o Time sampled ob o Chain of Custody mi O3 Date sampled btained from container.label saing date sampled ftined from container label Detailed Desription/Comments:

Did CAS inform client regarding receipt conditiop

/ No Temperature Authorization on Y / No HowlnWormed:

Phone / Fax / Mail/ Test Assignment Review Sh Y6 / No Who was contacted:

Remarks: Reiwdby-~~X~

ae t#_,$ji.IICoLSSM/FSI0w/IshwPBCVRwy/onomS C o n ti e n ta l 525 N. 8th Street, Salina, KS 67401 (785)827-1273 Fax (785)823-7830 www.cas-lab.com CHAIN OF CUSTODY RECORD Continental Shipping Order Number: Client/Reporting Information Invoice Information PARAMETERS/CONTAINER TYPE COMMENTS Company Name: w C N 0 r Company Name: Addr~o Address: State: Zip: City: State: Zip: c t: E-mail: Contact E-mail: ill,,L Loq~~os ,O, t. as~j'ck& c. o, Phone Number: Faxlqumber:

Phone Number: Fax Number: W2-gll.I4

-R9o 1 (A La -_ __4.__%A_1!9_

4't C S~a1n~ers'X me:(Printed Loc110 0ts,&c Purchase Order Number: O0" b 2 0 o Project Number.Project Name: I i-4 0.5 Number,, (Pres~e d&AIle UIz z~x 2z SAMPLE IDENTIFICATION (30 Charact less)Matrix (Sample Type)IU Regulatory Program Date Sampled Time Sampled rO v Y ~ i o)qu --__ X .---__ 9"_ )c Reultoy rora: NDE, RR =riigWater, S=503 Sludge, f--Other (rOeta e If m..oandard Rosh & Emergency su-ol t ,,idith,,el ,e" rhiiW Regulatory Program: :=NPDES, =RCRA, [=Drinking StamrdTAT:(15 wkln days) Rush TAT. (5 working day%) Emeyen, TAT: (3 werking days)Matrix Dff=Drinking Water, GW--Ground Water, IW=Waste Water, W=Wipe, S=Solid/Soil, B:,=Sludge, .A=Air, 01. = Oil/Organic Liquid, D =Other R9I Q HE YDATE-. TIME: RECEIVED BY: DATE: TIME--V --- 43--RELINQ4SHED BY: DATE: TIME: RECEIVED BY: DATE: TIME: ECEIV AT LABSBY DATE. TiE. SHIPPEDRVIA:

SEAL 0: (n AIRBILL SEAL DATE: J_-.

9 0 6 Sontinental Weltical Servi:s.Ic 06/26/2005 Wolf Creek Nuclear Operating Co.Attn: Ralph Logsdon P.O. Box 411 Burlington, KS 66839 Date Received:

06/14/2005 Continental File No.: 5796 Continental Order No.: 12807 P.O./Project No: 0701240 Purchase Auth: 701240/7

Dear Mr. Logsdon:

This laboratory report consisting of 8 pages contains for the following samples: the analytical results CAS LAB ID #05060679 05060680 SAMPLE DESCRIPTION 004 CCL SAMPLE TYPE Liquid Liquid DATE SAMPLED 6/13/2005 6/11/2005 Continental is accredited by the State of Kansas through the National Environmental Laboratory Accreditation Program (NELAP). The results contained in this report were obtained using Continental's Standard Operating Procedures.

These procedures are in substantial compliance with the approved methods referenced and the standards published by NELAP.The Appendix and Quality Control sections are an integral part of this report and may contain data qualifiers.

All results are reported on a wet weight basis unless otherwise stated.Samples will be retained for thirty days unless Continental is otherwise notified.Thank you for choosing Continental for this project. If you have any questions please contact me at (800)535-3076.

CONTINENTAL ANALYTICAL SERVICES, INC.Clifford JX. er Technical Manager Brian T. aDonnell Project Manager Page: I P.O. Box 3737 -525 N. Bighth St. -Salina, KS 67402-3737 785-627-1273 800-535-3076 Pax 785-823-7830 KMX Envilcrntal Laboratory Accreditation No. B-10146 S-ontinental E;- ýAnalytical Services,.

Inc.Page: 2 L.9 Client: Wolf Creek Nuclear Operating Co.Attn: Ralph Logsdon P.O. Box 411 Burlington, KS 66839 Date Sample Rptd: 06/28/2005 Date Sample Recd: 06/14/2005 Continental File No: 5796 Continental Order No: 12807 Lab Number: 05060679 Sample

Description:

004 Analysis Antimony, Total Arsenic, Total Beryllium, Total Cadmium, Total (GFAA)Chromium, Total Copper, Total Hardness (Calculated)

Lead, Total Mercury, Total Nickel, Total Selenium, Total Silver, Total (GFAA)Thallium, Total Zinc, Total Ammonia, Total, as N Chloride Nitrate, as N pH Analysis Antimony, Total Arsenic, Total Beryllium, Total Cadmium, Total (GFAA)Chromium, Total Copper, Total Hardness (Calculated)

Lead, Total Mercury, Total Nickel, Total Selenium, Total Silver, Total (GFAA)Thallium, Total Zinc, Total Date Sampled: 06/13/2005 Time Sampled: 0820 Concentration ND(0.006)ND(0.005)ND(0.004)ND(0.001)ND(0.010)ND(o.020)225.ND(0.003)ND(0.0002)

ND(0.040)ND(0.005)ND(0.002)ND(0.002)ND(0.020)NDW(0.10)34.6 ND(0.1) QC 8.54 H units mg/L mg/L mg/L mg/L mg/L mg/L mg/L as CaC03 mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L Std. units Book/Page 5983/25 5983/23 5979/26 5983/31 5979/26 5979/26 5979/26 5983/27 5672/255 5979/26 5983/22 5983/29 5983/32 5979/26 5892/277 5966/45 5966/43 5936/48 Date Prepared 06/20/2005 06/20/2005 06/16/2005 06/20/2005 06/16/2005 06/16/2005 06/16/2005 06/20/2005 06/20/2005 06/16/2005 06/20/2005 06/20/2005 06/20/2005 06/16/2005 Date Analyzed 06/22/2005 06/20/2005 06/17/2005 06/23/2005 06/17/2005 06/17/2005 06/16/2005 06/22/2005 06/21/2005 06/17/2005 06/20/2005 06/23/2005 06/23/2005 06/17/2005 QC Batch 050620-2 050620-1 050616-6 050620-3 050616-6 050616-6 050616-7 050620-3 050620-4 050616-6 050620-1 050620-3 050620-3 050616-6 Inst.Batch G1I173 IGF2171 91P2167 IGF1174 9IP2167 91P2167 121P2167 IGP1173 5MA1172 9IP2167 1GF2171 1GF1174 1GF1174 9IP2167 Analyst Method(s)KAM 7041 MAG 7060A KMW 6010B KAM 7131A KKK 6010B KMW '6010B KMW 6010 & SM 2340B KAM 7421 CRD 7470A KMD 6010B MAO 7740 KAM 7761 KAM 7841 KMW 6010B-Continued-P.O. S=~ 3737 -525 V. Eighth St. -Salina, 98" 6 7 4 0 2-3 3737*t 785-827-1273 800-53S-3076 Fax 7S5-823-7830 ME Envirmewntal Lboratory No. B-10146 .AdIUt2cOlerelre.

Inr.

I I'~1'3;El 9 0 Client: Wolf Creek Nuclear Operating Co.Attn: Ralph Logsdon P.O. Box 411 Burlington, KS 66839 Page: 3 Date Sample Rptd: 06/28/2005 Date Sample Recd: 06/14/2005 Continental File No: 5796 Continental Order No: 12807 Date Date Analysis Prepared Analyzed Ammonia, Total, as N N/A 06/17/2005 Chloride N/A 06/16/2005 Nitrate, as N N/A 06/14/2005 pH N/A 06/14/2005 Furnace Metals Total Preparation Method ICP Metals Total Preparation Method Mercury Total Preparation Method Total As & Se by GFAA Preparation Method Antimony Total Preparation Method Calculated as Hardness Preparation Method QC Batch 050617-2 050616-1 050614-1 050614-1 Inst.Batch 050617-3 050616-1 050614-3 050614-1 Analyst Method(s)MLL SM 4500-NH3(H)

JDL 300.0 JDL 300.0 SRE 9040B Metals/3020A 200.7/3010A SM 3112B/7470A 206.2/270.2/7060/774 200.7/3005A 200.7/60109 Conclusion of Lab Number: 05060679 Lab Number: 05060680 Sample

Description:

CCL Analysis Chloride Nitrate, as N Date Sampled: 06/11/2005 Time Sampled: 1600 Cqflcentration'l "35 !Units mg/L mg/L Book/Page 5966/43 5966/43 Analyst Method(s)JDL 300.0 JDL 300.0 Analysis Chloride Nitrate, as N Date Prepared N/A N/A Date Analyzed 06/14/2005 06/14/2005 QC Batch 050614-1 050614-1 Inst.Batch 050614-2 050614-2 Conclusion of Lab Number: 05060680 ND(), where noted, indicates none detected with the reporting limit in parentheses.

P.O. Box 3737 -525 N. Eighth St. -Salina, K8 67402-3737 785-827-1273 800-535-3076 Pax 78S-823-7830 Continenta I5113 Enviroanrntal Laboratory AcCreditatiio No. 3-10146 %Q AnnltWceo Serviwm. Inc.

E D)SContinental Page: 4 Client: Wolf Creek Nuclear Operating Co.Attn: Ralph Logsdon P.O. Box 411 Burlington, KS 66839 Date Sample Rptd: 06/28/2005 Date Sample Recd: 06/14/2005 Continental Pile No: 5796 Continental Order No: 12807 AI 0.QC -QC data qualifiers were noted. See the attached QC report.H -Regulatory analytical holding time for this analysis was exceeded.The following table presents the date and time sampled, the date and time analyzed, and the total time elapsed for each analysis with an EPA recommended holding time of forty-eight hours or less DATE/TIME DATE/TIME ELAPSED CAS LAB ID # ANALYSIS SAMPLED ANALYZED HRS:MIN 05060679 Nitrate, as N 06/13/2005 0820 06/14/2005 1904 34:44 05060679 pH 06/13/2005 0820 06/14/2005 1415 29:55 05060680 Nitrate, as N 06/11/2005 1600 06/14/2005 1607 72:07 All samples were received at the recommended temperature of less than 6 degrees Celsius.-Conclusion of Laboratory Report-P.O. Box 3737 -525 N. ,ighth St. 1 7 785-827-1273 800-535-3076 Faz 785-823-7830 Contie ntal M5)E Enviroomsntal Laboratory Accreditation No. 3-10146 AnalUtical

'Services.

Inc.

.Continental WL91!Jtical Servire:.

Inc, Quality Control Report Batch Summary Client: Wolf Creek Nuclear Operating Co.Attn_ Ralph Logsdon P.O. Dox 411 Burlington, K8 66839 Page t 5 Date Reported:

06/28/2005 Date Sample Receiveds 06/14/2005 Continental File NO: 5796 Continental Order No: 12807TeSt Teatm OC Batch Method Blank LCS MS Lab No.8L304 Beryllium, Total 050616-6 050616BLK6 050616LC86 05060685M4 w1o308 Chromium, Total 050616-6 050616916 050616LC86 0S060685148 S.313 Copper, Total 050616-6 0506161R.G6 050615LCBS6 0506068514M 8L336 Nickel, Total 050616-6 050616BLK6 050616,C86 05060685M LM369 Zinc, Total 050616-6 050616BLS6 050616LC86 05060685NS Lab numbers associated with this batch: 05060679 OL323 Hardness (Calculated) 050616-7 050616BL97 050616LC97 05060224)48 Lab numbers associated with this batch: 05060679 8L302 Arsenic, Total 050620-1 OS0620BLIC1 050620LCS1 90.50 Selenium, Total 050620-1 050620BI.11 050620LC81 05061011)S Lab numbers associated with this batch: 05060619 81,301 Antimony, Total 050620-2 050620B9.2 050620LCS2 0506101114 Lab numbers associated with this batch: 05060679 81.06 a um, Total (GWAA) 050620-3 050620B911 050620LCS3 050610111I4 5.328 Lead, Total 050620-3 050620BL93 050620LC83 05061011)48 8L371 Silver, Total WGFAA) 050620-3 0506209L1.3 050620LC83 SL.360 Thallium, Total 050620-3 050620BL93 050620LC83 05061011MS Lab numbers associated with this batch: 05060679 BL333 Mercury, Total 050620-4 050620B9J.4 050620LC84 05060040K5 Lab numbers associated with this batch: 05060679 61.110 Amnia, Total, am N 050617-2 050617BLX2 050617LC92 050606821S Lab numbers associated with this batch: 05060679 61502 Chloride 050614-1 050614BL9.1 0506141C81 0506055114 Lab numbers associated with this batch: 05060680 G".502 Chloride 050616-1 0506168L9.1 050616lC81 05060550MS Lab numbers associated with this batch: 05060679-Quality Control Report Contin.ed

-P.O. Box 3737 -525 N. Eighth St. -Salima, 15 67402-3737 785-827-1273 800-53S-3076 PaX 705-823-7830 Continenta l 1DF Environmental Laboratory Accreditation No. 8-10146 AnalUta:l

,.Services.

Inc.

I El 9 C)6 , ontinental-client: Wolf creek Nuclear Operating Co.P.O. Boz 411 Burligton.

18S 66839 Quality Control Report Batch Owunax 3.90: 6 Date Rteport.ed:

06/28/2005 Date 8a8ple Rteceive:

06/14/300S Conltinetaml File Do: 5796 Continental Order No: 12807 Teat Teatnamm QC Batch Method Blan]k LC NB La No.GLS05 Nitrate. an N 050614-1 050SIIBLII 0506141.081 OSOG055INS Labnub er. aemociated with thia batch: 05060679 05060680 01.,211 pM 050614-1 0506141, 081 05060685MB Lab umbera aaaociated wth thia batch:~05060679-Quality Control Continued

-* .o. ,=, 3737 -525 N. Bight,,S. ,auna, 18 6,402-3737

"- tn .785-827-1273 800-S35-3076 Faxr 785-923-7830 1~E ftle ta]18H[Z Bnlvironmental L /aboratory Accreditlation No. N-10146 1 Inc~];llr II A El[)9 II)C'Quality Control Report Meathod Blank, LOB, MS/MSD Data Client: Wolf Creex Nuclear Operating Co.Attn: Ralph LoWd=P.O. Box 411 Burlington, KS 66839 Page: 7 Date Reported:

06/28/2005 Date Sample Receiveds 06/14/2005 Continental File Not 5796 Continental Order Nos 12807 Spiked Sample SpLikd 8aG le Blank % Rec spike (I Reeouvey) opi- et ecision Dat Analyzim Data LOB i.4m4ta Level tits ME D= TL4-4tW Leve Units Ram Liit 00 match. 050614-1 1r sample analysed one 04/14/2003 spiked o emos 05040551 Chlorid 91(1.0) 101. 90.2-104 4.0 ag/L 110 SN 6.5-.118 400 .9/L ** 0.1 Nitzrate, w I (0.1) 96.1 89.2-107 2.0 us/L --1 76.5-1321 200 .9/L 4.9 ac Mtah$ 050614-1 fur ample analyzed one 06/14/2005 Spikd saples 05040685 OR N/A 7.00 4.90-7.10 7.0 Std. u 145 IN S Btd. u e 0.4 0c Satedes 050616-1 For Isample analyiied am 06/16/2005 Spiked smple 05060530 Chlorid D(1.0) 97.7 90.2-104 4.0 MG/L 1N MN 66.6-116 400 ag/L ** .1 0C 1latche 050616-6 0ro samples3 pZepared one 06/16/2005 pik"u sIample. 05060685 Beryllim, Total 50(0.004) 100 87.3-105 0.5 mg/L MN 1 0 84.7-105 0.5 mg/L ** 6.3 czms.sim, Total 30(0.010) 101 68.1-109 0.5 mg/L IN SI 5.4-109 0.5 ag/L ** 7.4 apper. Total W0(0.020) 103 67.5-110 0.5 g/L MIS UN 87.0-114 0.5 ag/i m. 0.5 mrckal. Total 30(0.040) 101 90.6-105 0.5 ag/i .MIN 87.3-106 0.5 ag/L

6.0 mine, Total 91)(0.020) 99.1 87.7-105 0.3 ag/L me up 82.3-111 0.5 8 a/i ** 9.7 00 Imtch* 050616-7 Voe smplea prepared ous 06/16/2005 Spikdm slaem 05060224 3awf..*

3M(5.0) 101 68.4-110 337 ag/L a ON -85.1-111 237 as/i U ** 9.4 00 atohs 050617-2 low IaW ls analysed Omn 06/17/2005 Spiked satlple@ 05060682 ammonia, Total, a x W(0.10) 104. 93.6-112 1.0 ag/L MY 11 81.8-114 1.0 g/L ** 3.8 0 Mimtake 050620-1 91 9=WlUs prepared Ono 06/20/2005 spiked asmole$4Areio. Total 30(0.005) 104 83.1-114 0.05 ag/i su MU 00 mabch, 050620-1 aror sa*lm propar" on, 06/20/2005

@piked @aWple, 05061011 Imelms m, total W0(0.005) 101 04.7-112 0.05 ag/i Un UY 54.9-118 0.05 mg/L 9* 6.2 CC Retchs 050620-2 War Bowles; prespard one 06/20/2005 Spiked sapls 03061011 antlaW,, Total 30(0.006) 102 80.3-113 0.05 ag/L M UU 76.1-113 0.05 ag/L ** .8 0C Betakh 050620-3 w &maples pazpauzd mt 06/20/3005 Spiked Bolme#S1ilvr, Total (Gma&) 30(0.002) 90.0 65.4-113 0.01 Mf/L UIN M 00 &stake 050620-3 Vo propar" one 06/20/2005 Spikind i1oplm 05061011 camiumi. Total (wiaa) 3D(0.001) 106 83.4-119 0.0025 aIg/i UN UN 68.7-134 0.0025 ag/i ** 19.6 old, Total 31)(0.003) 109 86.9-112 0.05 mg/i MU UN 53.5-128 0.05 ag/i ** 6.6 Thallim. Total 30(0.002) 102 89.3-112 0.025 1ag/i UN UIN 57.2-130 0.023 ag/i e* 9.1 00 brtch* 050620-4 ow iomplete pzrepabed ant 06/20/2005 ampl., 05060840 rcury, Total m0(0.0002) 105 67.9-111 0.005 ag/L 0U uN 76.4-121 0.005 mg/L ** 6.6 Data Qualifiars:

W -The WMS/MD 8a21 aalymeY Were not performed on & mmamle from thin 0tinental odar Mtmher.N/A -Not Applicable 6 -iuta, not avamileble.

-Ro canno be calculated.-coaclusion Quality cntrol Raport-P.O. Boz 3737 -525 N. Eighth St. -Sal1ina, KS 67402-3737 Co t n ta 785-827-1273 000-535-3076 Fax 705-823-7030 EKLIIY KIMB Environmental Laboratory Accreditation No. 3-10146 --ArMItBrAd5eirvcew.

Inc.

I rLl[)(J 6 Quality Control Report Continuing Calibration Verification Data Bmunury Clients Wolf Creek Nuclear Operating Co.Attn Ralph Logsdon P.O. Box 411 Burlington, K] 66839 Page: 8 Date Reported:

06/28/2005 Date Sample Received:s 06/14/2005 Contintal File No: 5796 Coti-nsntal Order No: 12807 Analysis Aumonia, Total.pH'Chloride Chloride Nitrate, as N Analysis Antimony Arsenic Beryllium Beryllium Chromium Chromium Copper Copper Lead, Total Miercury.

Total Nickel Nickel Selenium, Total Zinc Zinc Silver Camdium Date of Znstrumnt Amount in Amount Analysis Batch ID Standard Detected as N 06/17/2005 No data qualifiers present for this 06/14/2005 No data qualifiers present for thisL 06/14/2005 No data qualifiers present for this 06/16/2005 No data qualifiers present for this 06/14/2005 050614-4 2.00 1.79 Samles associated with this Continuing Calibration Verification:

Laboratory Number Instrument Batch Samle Description 05060680 050614-2 CCL 05060679 050614-3.

004 units analysis.analysis.analysis.analysis.mg/L Percet 89.5 CL Percent Date of Analysis 06/22/2005 06/20/2005 06/17/200S 06/17/200S 06/17/2005 06/17/200S 06/17/2005 06/17/2005 06/22/2005 06/21/2005 06/17/200S 06/17/2005 06/20/2005 06/17/2005 06/17/2005 06/23/200s 06/23/2005 Instrunt Amount in Amount Batch ID Standard Detected units No data qualifiers present for this analysis.No data qualifiers present for thits analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for thi analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.No data qualifiers present for this analysis.Data Qualifiers:

CL -The continuing calibration verification (CCV) standard recovery for this amlyte was below the method or SOP limit. The reported sample concentration may be biased low.-Quality Control Report Conclusion

-P.O. Box 3737 -525 N. Eighth St. -Salina., K 67402-3737 785-827-1273 800-535-3076 PVa 785-023-7830

]MM Environmantal Laboratory Accreditation No. 8-10146 ý -.2- AnsalcUUU.

Servtce. Inc.

COOLER / SAMPLE RECEIPT FORM (C/S RF) File No.: -P (0 Order No.: Client Name: C AAT Sample ID: Date/Time coolerrceived: (o 11LL4L/.VSi (qq1:i0t By: Unpacked By: I~k .Date entered into LHASt: Red Warning Sticker Applied: Yes re) By: Date: / J/Samples Screened with Geiger Counter.&

N vtG Date. A-j0 itjO/CAS Cooler #: gq I/Client's Cooler/Box/Letter/Hand Delivered Cooler Identification:

Other Cooler Size: Small 1/ / Large I NA Delivered By: 1JFedX/DHL/ASAP/Land Air Exp/Field Svcs/Mail/Walk-In/Other Air Bill Number: Custody Seal: 6 i tact 'Broken) Absent Seal No: I_____9____U_

Seal Name: Seal Date: '1 Seal matches Chain of Custody: Yes / No A Type of Packing Material:

Blue u3Ieo am/Paper/Peanuts/

Vermiculite/

NA Cooler Temperature

(°C): Original Reading - C Corrected Reading -°C Temp. By: Temp. Blank.U Poured__ Surface G P M Thermo. ID No.: (_ ..Thermo. Correction Factor (*C): Sample Receipt Discrepancies:

'I o E3 Yes (See detail below)3 Chain of Custody not present 13 Sample excluded from Chain of Custody:*3 Information obtained from: 3 Broken or Leaking Containers:

Purchase Order/Letter received with samples El Sample listed on Chain of Custody, not received: r'

Container label absent El Sample description on container label different fhrm SChain of Custody incomplete Chain of Custody: 13 Chain of Custody missing time sampled El Air bubbles in VOA vials: El Time sampled obtained from containerlabel o3 Chlin of Custody missing date sampled 13 Date sampled obtained from container label Detailed Description/Comments:

lDid CAS inform client regarding receipt a~ndltlO 3)/ No Temperature Authorization.

on No How nformed: Phone / Pax / Mail / Test Assignment Review Sheets es )No Who was contacted:

Remarks: Reviewed by: Date.L W.#SoliWo 4/C91s/ofto5 1: 525 N. 8th Street, Salina, KS 67401 S Continentaa S78)87e2, In30 Fax-75)2c73~~~~~~~~Analgtlcal Services, Inc. wwsm w, ,.cs-lab.com(

3 '07 a* )s -o CHAIN OF CUSTODY RECORD Continental Shipping Order Number: 1 1 9 Ctient/Reporting Information Invoice Information PARAMETERS/CONTAINER TYPE COMMENTS U Company Name: wJ c IM t C.Company Name: 0. cat3 (_.Apress: ,Address: State: Zip: City: Slate: Zip: tact: E-mail: Contact. E-mail: Phone Number: Fax Number: Phone Number: Fax Number.(ow -3 6 ___8__1____l______________-A 2 z*5~C-5%S I~ ýrs me:(Printed) olL UceZ4QI, Mqlre Purchase Order Number: rzn % 1 44-~ .- I---- ~IPA Project Number: IProject Name: 0 Number tiPreserved tktthn 0 Tz z SAMPLE IDENTIFICATION 3O0 Characters or less)Malrl.(Sample Type)Regulatory Progran Thime Sampled 0 C-C L LJJYIio _ _xtr e~e ifmmrsnxUndjard tuans~umL Rush & Einrryvlnry su"jc klad&ditikmal uharl~)Regulatory Program: N=NPDES, R=RCRA, D=Drinlking Water, S.L=503 Sludge, Q--Other S(,s i ftandard TAT: (l5vkfngdays RushTAT:-,( rrkngd.ix Em.erng*n TAT: (3 w,, kingda.y')

Matrix (Sample Type): DfW=Drinking Water, GW--Ground Water, MW=Waste Water, W=Wipe, S=Solid/Soil, SL,-Sludge, .A=Air, QL= Oil/Organic Liquid, Q=Other[RE Q E DATE: TIME: RECEIVED BY: DATE: TIME: RE'LINQU MEDBY: DATE: TIME: RECEIVED BY: DATE: TIME: 0ECEIVED TLABBY: DATE- TIME: HI PPED VIA: SEALS:-(7~) IRBILL- SEAL DATE: a-C0C1M150.XLS/Il.ANX/4/?9tZDI E[I I C]0 November 2006 Circulating Water Bromination Schedule TE File: 42309 LOGDATE ENTRY 11/01/2006 8:10:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/01/2006 9:55:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/02/2006 8:00:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/02/2006 9:45:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/03/2006 8:05:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/03/2006 9:50:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/04/2006 7:55:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/04/2006 9:40:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11105/2006 8:22:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 110.11/05/2006 10:07:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/06/2006 8:15:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL-l10.11/06/2006 10:00:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/07/2006 7:48:20 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/07/2006 9:33:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/08/2006 8:10:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/08/2006 9:53:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/09/2006 7:50:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/09/2006 9:35:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/10/2006 8:15:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL-l10.11/10/2006 10:00:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/11/2006 8:10:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/11/2006 9:55:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.:..11/12/2006 8:25:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 1J0.11/12/2006 10:10:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL-110.11/13/2006 8:17:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/13/2006 10:02:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL-I 10. -11/14/2006 8:15:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL-i 10..11/14/2006 10:00:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL-I 10...11/15/2006 7:48:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/15/2006 9:33:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/16/2006 7:50:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11116/2006 9:35:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/17/2006 7:55:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/17/2006 9:40:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/18/2006 8:10:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/18/2006 9:55:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/19/2006 8:05:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/19/2006 9:50:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/20/2006 7:55:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/20/2006 9:40:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/24/2006 8:20:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/24/2006 10:05:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/25/2006 7:45:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/25/2006 9:30:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/26/2006 7:45:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/26/2006 9:30:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/27/2006 7:50:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/27/2006 9:35:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.11/30/2006 8:10:00 AM Site Watch commenced chemical addition to circ. water lAW SYS CL- 10.11/30/2006 9:55:00 AM Site Watch secured chemical addition to circ. water lAW SYS CL- 10.

G H E b ([)November 2006 Service Water Bromination Schedule LOGDATE ENTRY 11/01/2006 10:10:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-110.11/01/2006 4:10:00 PM Site Watch secured chemical addition to service water lAW SYS CL-110.11/02/2006 10:26:00 AM Site Watch commenced chemical addition to service water lAW SYS CL- 10.11/02/2006 4:26:00 PM Site Watch secured chemical addition to service water lAW SYS CL-l10.11/04/2006 9:55:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-I 10.11/04/2006 3:55:00 PM Site Watch secured chemical addition to service water lAW SYS CL-i 10.11/05/2006 10:25:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-1 10.11/05/2006 4:25:00 PM Site Watch secured chemical addition to service water lAW SYS CL-I 10.11/06/2006 10:25:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-1 10.11/06/2006 6:25:00 PM Site Watch secured chemical addition to service water lAW SYS CL-I 10.11/07/2006 9:46:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-1 10.11/07/2006 3:08:00 PM Site Watch secured chemical addition to service water lAW SYS CL-I 10.11/08/2006 10:10:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-110.11/08/2006 4:10:00 PM Site Watch secured chemical addition to service water lAW SYS CL-110.11/09/2006 9:50:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-1 10.11/09/2006 3:50:00 PM Site Watch secured chemical addition to service water lAW SYS CL-1 10.11/10/2006 10:15:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-110.11/10/2006 4:15:00 PM Site Watch secured chemical addition to service water lAW SYS CL-1 10.11/11/2006 10:10:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-110.11/11/2006 4:10:00 PM Site Watch secured chemical addition to service water lAW SYS CL-I 10.11/12/2006 10:20:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-1 10.11/12/2006 4:20:00 PM Site Watch secured chemical addition to-service water lAW SYS CL-I 10.11/13/2006 10:15:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-b10.11/13/2006 4:15:00 PM Site Watch secured chemical addition to service water lAW SYS CL-1 10.11/14/2006 10:12:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-110.11/14/2006 4:12:00 PM Site Watch secured chemical addition to service water lAW SYS CL-1 10.11/15/2006 9:47:00 AM Site Watch commenced chemical addition to Service Water lAW SYS CL-1 10..11/15/2006 3:47:00 PM Site Watch secured chemical addition to service water lAW SYS CL-110.11/16/2006 9:54:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-I 10.11/16/2006 3:54:00 PM Site Watch secured chemical addition to service water lAW SYS CL-1 10.11/18/2006 10:15:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-110.11/18/2006 4:15:00 PM Site Watch secured chemical addition to service water lAW SYS CL-110.11/19/2006 10:05:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-I 10.11/19/2006 4:05:00 PM Site Watch secured chemical addition to service water lAW SYS CL-i 10.11/24/2006 10:38:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-I 10.11/24/2006 4:38:00 PM Site Watch secured chemical addition to service water lAW SYS CL- 10.11/25/2006 9:45:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-I 10.11/25/2006 3:45:00 PM Site Watch secured chemical addition to service water lAW SYS CL-1 10.11/26/2006 9:44:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-1 10.11/26/2006 3:44:00 PM Site Watch secured chemical addition to service water lAW SYS CL-I 10.11/27/2006 9:48:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-I 10.11/27/2006 3:48:00 PM Site Watch secured chemical addition to service water lAW SYS CL-1 10.11/28/2006 10:28:00 AM Commenced macrofoul treatment of 'A' ESW/Service Water lAW SYS EF-300 11/29/2006 8:15:00 AM Secured macrofoul treatment to A ESW/Service Water lAW SYS EF-300 11/30/2006 10:10:00 AM Site Watch commenced chemical addition to service water lAW SYS CL-1 10.11/30/2006 4:09:00 PM Site Watch secured chemical addition to service water lAW SYS CL-1 10.

I (1 E[)Cj 6'(7 November 2006 Wastewater Discharges Through Outfall 003A & 003B LOGDATE ENTRY 11/01/2006 8:57:00 PM Commenced discharge of WWT Basin A to the environs per LRP # 2006051 11/01/2006 10:30:00 PM Commenced discharge of THF04A to the environs per LRP # 2006062 11/02/2006 1:30:00 AM Secured discharge of THF04A, the total volume released was 14,153 gallons.11/02/2006 2:15:00 AM Secured discharge of WWT Basin A, the total volume released was 136,000 gallons.11/03/2006 9:59:00 AM Commenced discharge of WWT Basin B to the environs per LRP # 2006051.11/03/2006 1:50:00 PM Secured discharge of WWT Basin B, the total volume released was 144,000 gallons.11/04/2006 10:07:05 PM Commenced discharge of all four S/G's to the environs per LRP # 2006050.11/04/2006 11:56:00 PM Secured Discharge of all four S/G's.11/05/2006 3:35:00 PM Commenced discharge of WWT Basin A to the environs per LRP # 2006051 11/05/2006 8:50:00 PM Secured discharge of WWT Basin A, the total volume released was 142,000 gallons.11/06/2006 8:30:00 PM Commenced discharge of THF04B to the environs per LRP # 2006069.11/06/2006 10:28:00 PM Secured discharge of THF04B, the total volume released was 13,899 gallons.11/07/2006 12:30:40 PM Commenced discharge of SGBD to lake per SYS BM-126.11/07/2006 4:25:00 PM Commenced discharge of THFO4A to the environs per LRP # 2006070.11/07/2006 5:11:00 PM Commenced discharge of WWT Basin B to the environs per LRP # 2006066.11/07/2006 7:15:00 PM Secured discharge of THF04A, the total volume released was 14,408 gallons.11/07/2006 8:41:00 PM Secured discharge of WWT Basin B, the total volume released was 128,000 gallons.11/09/2006 4:02:00 AM Commenced discharge of WWT Basin A to the environs per LRP # 2006066.11/09/2006 10:05:00 AM Secured discharge of WWT Basin A, the total volume released was 144,000 gallons.11/09/2006 11:40:00 AM Commenced discharge of THF04A to the environs per LRP # 2006071.11/09/2006 12:50:00 PM Secured discharge of THF04A, the total volume released was 11,018 gallons.11/10/2006 1:20:00 AM Commenced the discharge of WWT Basin B, to the environs per LRP # 2006066.11/10/2006 1:25:00 AM Commenced discharge of THF04B to the environs per LRP# 2006072 11/10/2006 2:55:00 AM Secured the discharge of THFO4B, the total volume released was 14,577 gallons.11/10/2006 9:52:00 AM Secured discharge of WWT Basin B, the total volume released was 144,000 gallons.11/13/2006 10:35:00 AM Commenced discharge of THF04B to the environs per LRP # 2006073 11/13/2006 1:20:00 PM Secured discharge of THF04B, the total volume released was 14,238 gallons 11/15/2006 10:15:00 AM Commence discharge of WWT Basin A to the environs per LRP # 2006066.11/15/2006 10:35:00 AM Commenced discharge of THF04A to the environs per LRP # 2006074 11/15/2006 1:20:00 PM Secured discharge THF04A, the total volume released was 14,238 gallons.11/15/2006 3:25:00 PM Secured discharge of WWT Basin A, the total volume released was 122,000 gallons.11/17/2006 10:00:00 AM Commenced discharge of WWT Basin B to the environs per LRP # 2006066.11/17/2006 Commenced discharge of THF04B to the environs per LRP # 2006075 11/17/2006 1:20:00 PM Secured discharge of THF04B, the total volume released was 13,051 gallons.11/17/2006 2:03:00 PM Secured discharge of WWT Basin B, the total volume released was 136,000 gallons.11/28/2006 7:45:00 AM Commenced discharge of WWT Basin A to the environs per LRP # 2006066.11/28/2006 12:55:00 PM Commenced discharge of THF04B to the environs per LRP # 2006076.11/28/2006 1:10:00 PM Secured discharge of WWT Basin A, the total volume released was 146,000 gallons.11/28/2006 2:00:00 PM Secured discharge of THF04B, the total volume released was 10,000 gallons.11/29/2006 10:30:00 AM Commenced discharge of WWT Basin B to the environs per LRP # 2006066.11/29/2006 3:30:00 PM Secured discharge of WWT Basin B, the total volume released was 126,000 gallons.11/30/2006 8:55:00 AM Commenced discharge of THF04A to the environs per LRP # 2006077.11/30/2006 11:00:00 AM Secured discharge of THF04A, the total volume released was 14,407 gallons.

Enclosure 5 to ET 07-0001 Fishery Monitoring Reports 2002 Fishery Monitoring Report 2003 Fishery Monitoring Report and 2004 Plan 2004 Fishery Monitoring Report And 2005 Plan 2005 Fishery Monitoring Report And 2006 Plan 3 WOLF CREEK GENERATING STATION Wolf Creek Lake 2002 FISHERY MONITORING REPORT Prepared by: Supervisor Regulatory Services Approval: Manager Regulatory Affairs Approval: Ken Hughes Tony Harris~1 3/20/03 5-6-03 Date 5/8/2003 Date-I.

EXECUTIVE

SUMMARY

The results obtained from fishery monitoring of Wolf Creek Lake (WCL) during 2002 indicate that the potential for gizzard shad impingement at the cooling water intake screens has remained low. The primary objective of the monitoring was to measure fish population dynamics to determine shad impingement potential.

The fishery assessments targeted gizzard shad, the predator species that feed on them, and the predator-prey interactions.

Catch frequencies of young gizzard shad decreased slightly, and remained low during 2002.Consequently, no impingement problems developed.

A higher proportion of larger adults were likely from the increased production in 2001.The 2002 monitoring revealed that the predator populations showed favorable signs for continued shad control. Predator populations, as a whole, showed signs of being prey limited.Growth rates and body conditions tended to be low. Continuous declines in these areas would raise concerns, because it is important that the predator populations remain viable so that shad control continues.

Catch rates were similar to past years', and recruitment was evident for many predator species. Predator populations assessed were white bass, wiper hybrids, largemouth bass, smallmouth bass, white crappie, and walleye.Angling impacts to the predators' shad control benefits were also assessed.

The catch-and-release philosophy being stressed at WCL has made the limited harvest compatible with continued shad control. Continued low body condition of smallmouth bass and walleye justified altering the length and creel limits for these species. Innovative length limits were put in place for smaltmouth bass and walleye in an attempt to promote larger individuals.

Monitoring data will be important to ensure no adverse impacts to the fishery results from angler harvest.2 TABLE OF CONTENTS EXECUTIVE SUM M ARY ..............................................................................................................

2 2002 FISHERY M O NITO RING REPO RT ................................................................................

5 1.0 INTRO DUCTIO N ....................................................................................................

5 2.0 M ETHO DS ....................................................................................................................

5 3.0 RESULTS AND DISCUSSIO N ...............................................................................

5 3.1 PREDATO R/PREY INTERACTIO NS ...............................................................

6 3.1.1 G izzard Shad ......................................................................................

6 3.1.2 Predators

.............................................................................................

7 3.2 ANGLER HARVEST IM PACTS ........................................................................

8

4.0 CONCLUSION

S AND MANAGMENT IMPLICATIONS

..........................................

10 5.0 LITERATURE CITED .............................................................................................

11 3 List of Tables Table 1. Fishery sampling effort by gear type used at Wolf Creek Lake during 2002 ...... 13 2. Relative Abundance and Percent Biomass of Selected Fish Species in W olf C reek Lake ............................................................................

..14 3. Catch-per-unit-effort of Selected Fish Species in Wolf Creek Lake ..............

16 4. Proportional Stock Density and Relative Stock Density for Selected Fish S pecies in W olf C reek Lake ....................................................................

17 5. Relative Weight of Selected Fish Species in Wolf Creek Lake ............

19 6. Fish Released and Harvested By Angler at Wolf Creek Lake ....................

20 List of Figures Figure 1. Fishery sampling locations on Wolf Creek Lake .....................................

21 2. Gizzard shad catch-per-unit-of-effort (CPUE) from October small-mesh gill net complements and standard mesh gill net complements

.............................

22 4 2002 FISHERY MONITORING REPORT

1.0 INTRODUCTION

This report presents and interprets the results of fishery monitoring activities on Wolf Creek Lake (WCL), and demonstrates that the fishery has functioned as desired through 2002. Data available from 2001 are also presented.

The majority of fishery sampling in 2001 was not completed due to the events of September

11. Long-term fishery trends were tracked to identify change and forecast potential impacts to the efficient and safe operation of Wolf Creek Generating Station (WCGS).Excessive fish impingement on intake screens can cause costly equipment damage and power production outages. Typical impingement problems on intake screens develop because gizzard shad cannot avoid intake flows when they naturally become weakened, and eventually die, as winter water temperatures fall below approximately 400 F (Bruce NGS 1977, Ontario Hydro 1977, Olmstead and Clugston 1986, White et al 1986). Arkansas Nuclear recently experienced such problems with excessive threadfin shad impingement during a winter die-off in late 1998 (NRC 1999; C. Adams, Arkansas Nuclear, personal communication).

Early during WCL construction, it was expected that shad could not be excluded from, and would flourish in the lake. Consequently, an aggressive stocking program was completed (KG&E 1984), and the resulting fishery has not caused impingement problems at WCGS Circulating Water Screen House.Public angling was allowed for the first time starting on October 1, 1996. Restrictive creel and length limits to protect the current populations were established jointly with the Kansas Department of Wildlife and Parks (KDWP). The catch-and-release strategy appears to have succeeded with no detrimental changes to the fishery observed through 2002.2.0 METHODS The methods employed during 2002 allowed for continued analyses of important long term trends. Electrofishing, trap (Fyke) netting, and gill netting were used at long-term sites on WCL (Figure 1). Small-mesh gill netting replaced shoreline seining in 1998 to better assess young-of-year (YOY) gizzard shad densities and recruitment (Boxrucker et al -1991). Important species to the fishery were targeted when expected to be efficiently sampled.Sampling efforts are listed in Table 1. Fish sampled were weighed to the nearest gram, and measured (total length, TL) to the nearest millimeter.

Proportional stock density (PSD, Anderson 1980), incremental relative stock density (RSD, Gablehouse 1984), and relative weight (Wr, Wege and Anderson 1978) were indices used. Length-weight equations adopted by KDWP were used. Gill net efficiency adjustments to the PSD and RSD indices were completed for gizzard shad, white bass, and walleye (Willis et al 1985)3.0 RESULTS AND DISCUSSION A total of 23 different species were collected in 2002. All 23 have previously been sampled in the past. The relative abundance and percent biomass of each species collected were similar to past years (Table 2).5 3.1 PREDATOR/PREY INTERACTIONS Typical prey species tend to produce a large number of young each year. Characteristics of an annually cropped prey population, such as in WCL, would be a high percentage of larger, older individuals, fast growth of YOY, and good health of individuals.

The number of fish making it to reproductive age (recruitment) would also be low. A concern with excessive cropping would be if the number of reproducing adults became too low to produce enough young to support the predators.

This would result in a subsequent loss of the predators.

Characteristics of predator populations in a low-prey fishery would include low recruitment due to cannibalism or predation, slow or no growth of adults, large percentages of older individuals, and poor health of adults. Difficulty in producing trophy size individuals would also be evident.3.1.1 Gizzard shad The potential for excessive gizzard shad impingement remained small due to relatively low YOY densities going into the winter months. The shad appear to be limited by predation, as indicated by the population indices of the predator species.Gizzard shad typically has been an important forage species in most reservoirs (Carlander 1969, Pflieger 1975, Stein and Johnson 1987, Colvin 1993). For shad to be compatible with WCGS operation, low YOY shad densities must be maintained.

Periodic recruitment of shad young to reproducing adults also must occur to maintain the predators, which in turn control shad numbers. These conditions currently exist in WCL, and benefit WCGS.Gizzard Shad YOY Densities and Recruitment:

Catch-per-unit-effort (CPUE) for the small-mesh gill nets was lower than last sampled in 2000 (Figure 2). Numbers of stock size shad captured in the standard mesh gill nets also declined (Table 3). This coupled with the rise in larger shad (Figure 2) caused the PSD index to rise to 96 (Table 4). The larger proportion of greater than stock size shad was likely due to adults recruited from the increased YOY shad sampled in 1998 or 2000.Length frequencies of shad sampled by the small-mesh nets showed YOY shad less than 120 mm, which are sizes most vulnerable to winter die-off and intake impingement (White et al 1986). In WCL, not all of the YOY gizzard shad were sizes considered vulnerable to impingement.

October sampled YOY in WCL typically ranged from 90 to 230 mm (4 to 9 inches). First year shad growth to lengths between 90 and 150 mm were normal. In some instances, WCL lengths up to 230 mm were identified.

These demonstrate growth rates that are considered among the faster growing rates for shad in the United States (Carlander 1969). Earlier than normal spawning in the heated discharge area of the lake likely contributes to the high YOY growth rate in WCL (WCNOC 1992).To confirm growth of YOY shad up to 230 mm, back-calculated lengths determined from scale samples collected from large adults during the October, 1998 gill netting showed that the majority of WCL shad formed their first scale annuli when over 200 mm. Similar back-calculated lengths from WCL scale samples were present for 12 6 large adult shad, which showed first year growth from 126 to 228 mm with an average of 174 mm (Colvin 1995). First scale annuli for shad typically have been during the winter months (Carlander 1969). It was possible shad that spend their first winter in the heated discharge continued to grow throughout the year, and thus did not form first-year annuli. This would explain the high rate of first-year growth from back-calculated scale analyses.Young-of-year gizzard shad characteristically grow quickly to sizes large enough to escape significant predation.

This has been considered a detriment to sport fish management (Putnam and Devries 1994). In WCL, though, the shad's ability to grow too large for the predators likely contributes to the observed long-term balance between shad and predators.

The scale aging analyses discussed above supports this. Many of the reproducing sized shad were recruited from the faster growing YOY shad. These larger, first-year shad were likely spawned earlier than normal, and their growth was enhanced by a longer growing season (NRC 1982, WCNOC 1992). Scale aging analyses also showed that few of the smaller (90-150 mm TL)YOY shad survived to recruit to reproducing size. Heavy predation and mortality due to winter stress were likely causes. Once the larger YOY grew large enough to escape the majority of WCL predators, consumption of the smaller shad should have intensified from late summer through early fall. Also, since the shad grew large enough to reduce their susceptibility to winter-kill mortality, their potential to cause plant impingement problems likewise was reduced. Apparently, the faster growing shad avoid predation and winter stress mortality, and comprised the majority of the brood stock. Following this logic, similar maintenance of large reproducing shad densities may not be as likely to occur in the absence of WCGS thermal discharges.

3.1.2 Predators Wolf Creek Lake predators showed signs of being prey limited, which contributes to the reduction of impingement potential at the station's intake screens. Sufficient recruitment has been occurring for all game fish except largemouth bass, which has been low since 1992. Predator densities were similar to 2000, except for the declines in smallmouth bass and Walleye (Table 3). Body conditions were generally low for game species. Consistent body conditions, and low shad catch rates were indicative of no angling impacts.White Bass: A wide range of white bass sizes was sampled in WCL, which was similar to recent years. The mid-range PSD index indicates continued recruitment (Table 4). White bass density and average body condition remained similar to previous years as evidenced by the gill net catches (Tables 3 and 5).Wiper: Wiper hybrids appear to be limited in how large they can grow, likely due to current densities of shad. Stockings grew well up to the 510 to 560 mm TL size range (20 -22 inches), but rarely into the trophy size range (Table 4). Their declining body conditions (Table 5) may be indicative of aging fish, as well as low prey availability.

Because the current year-classes are aging and the CPUE has fallen in 2002, stocking another year class in 2004 is recommended to maintain a wiper presence in the lake. A subsequent 2005 stocking may be necessary.

7 Smallmouth bass: The smallmouth bass population was well represented by various size classes with consistent mid-range PSD's (Table 4), indicating good recruitment.

Average health decreased slightly with a Wr value of 82 (Table 5).The CPUE for fall shocked smallmouth bass declined sharply in 2002 (Table3).

The lack of 2001 fall data makes it difficult to determine if this is a trend or a result of sampling variation.

An increase in the angler catch and release rate and (Table 6), and the low Wr (Table 5) would tend to indicate that densities had not decreased.

Electrofishing results in 2003 should determine if a declining trend has occurred.Largemouth bass: Catch frequencies of largemouth bass in WCL have declined greatly since the 1989 high (Table 3). The 2000 spring electrofishing catch of largemouth bass remained low. Little confidence should be placed in the population statistics, other than low catch rates, because of the small sample size. Recruitment may have increased evidenced by the lower PSD index in 2001 and 2002 (Table 4). Average health also improved (Table 5).White crappie: Fyke netting during the fall was used to assess the crappie population in 2000.Spring efforts were used in the past. The fall sample showed good percentages of larger individuals, but also sampled the intermediate sizes not typically caught in the past spring samples (Table 4). Catch rates declined slightly in 2002 (Table 3) while average body condition was excellent (Table 5).Walleye: The walleye population was well represented by individuals from several year classes in the fall gill nets (Table 4). Good recruitment has occurred since 1991, as evidenced by mid-range PSD's (Table 4). Walleye catch frequencies in 2002 declined similar to the smallmouth bass decline (Table 3). Continued high numbers of angler caught and released rates does not indicate a decline, and suggests sampling variation from the gill nets (Table 6). Because of the anglers' high preference for walleye and of its value in shad control, catch frequencies should continue to be monitored through 2003. Body conditions remained similar to past years (Table 5).3.2 ANGLER HARVEST IMPACTS When the lake was opened to public fishing on October 1, 1996, low creel and high length limits were established to ensure no angler impacts to the fishery. Leading up to that time, gizzard shad were showing signs of increased recruitment (Table 4), indicating potential rises in shad production and thus in impingement rates. This did not materialize, with subsequent monitoring showing both low shad recruitment and low body conditions of predators.

Consequently, it was determined that a harvest increase was possible for some species with little potential to increase impingement rates.8 White bass: A daily creel limit of five white bass > 12 inches was allowed through 2002. Length frequencies and CPUE show continued recruitment, indicating that creel and length limits had no adverse impacts. White bass catch and release declined in 2002, probably due to lake closure during the early spring and late fall which are periods of higher angler catches (Table 6). The cyclic nature typical of many white bass populations may have contributed also. Angler harvest typically does not impact white bass due to their high recruitment rates and shorter life span. Consequently, the creel limit of five fish per day was modified to unlimited starting in 2003. This matches the KDWP creel for other Kansas lakes. The 12-inch minimum size limit was kept to aid anglers in discerning the similar looking wiper hybrids.Wiper: Wipers have been very important for controlling shad and the optimum size based on historic length frequency distributions has been between 500 and 600 mm (approximately 20-24 inches). Wiper populations were not self-sustaining, consequently WCGS has invested and plans to continue investing in replacement wiper stockings, on an as needed basis. The 24-inch length limit for angler harvest was set to protect the investment and to help ensure that the wipers will reach the preferred size range for controlling shad. Wipers larger than 24 inches have generally been older individuals that were not expected to survive much longer, and thus their removal would not impact shad control benefits.

The optimum size could change if higher numbers of shad increase wiper growth, thus exposing higher numbers to harvest. Angler catch and release rose slightly, likely due to the 2001 wiper class becoming larger, and thus more susceptible to capture (Table 6).However harvest rates remained low. Consequently, the current wiper creel limit of one per day, and minimum length limit of > 24 inches will remain unchanged.

Smallmouth bass: Smallmouth bass were the dominant shoreline predator and were abundant along the riprap. Good recruitment, coupled with low body conditions in 1999 and 2000 indicated room for a higher harvest rate. Consequently, the smallmouth bass length and creel limits were assessed and changed from one > 18 inches and one < 13, or two < 13 inches, to two fish per day, either < 13 or > 16 inches. These changes reflected KDWP's desire to have WCL continue to develop into a trophy smallmouth bass fishery. No detrimental impacts were identified as catch and release rates remained high and harvest remained unchanged (Table 6). Because of the high angler catch rate and continued low body condition (Table5), it was concluded that competition by smallmouth bass for available resources was high in WCL. There was also the desire by KDWP to continue to promote larger bass. Consequently, the size limit for 2003 was changed to two fish, either < 16 or> 20 inches. This was to promote harvest of smaller fish and protect larger sizes.Largemouth bass: Largemouth bass catch rates remained low through 2002. Setting the length limit at 21 inches was to allow essentially no harvest. No changes to the current length and creel limits were recommended.

White or Black Crappie: The population was characterized by consistent, high percentages of larger individuals (PSD typically

> 90, Table 4), and good to excellent condition indices (Table 5).9 Occasional dominant year classes common of many crappie populations also appear to be lacking. These conditions indicate low intraspecific competition, which was unusual and likely caused by high mortality of young crappie, possibly through predation.

Harvest of crappie < 14 inches may jeopardize current production and subsequent recruitment, and thus the population's contribution to gizzard shad consumption.

Consequently, the current crappie creel of two per day with minimum lengths of > 14 inches, will remain unchanged.

Walleye: Length frequencies indicate good recruitment of walleye since 1991 (Table 4). Low recruitment of shad, sustained walleye densities, and low body conditions of walleye justified increased harvest. Consequently, the creel and length limits were changed for 2001 from two per day > 18 inches, to two walleye, one of which must be > 18 inches.Angler harvest increased accordingly (Table 6). However, continued low body condition indicated continued high competition for prey resources.

In an effort to manage for larger walleye, it was recommended by KDWP and approved by WCNOC to change the size limit for 2003 to two fish, either < 18 or > 26 inches. This should increase harvest of smaller walleye, thus give room for remaining fish to grow into the protected size range. However, if harvest pressure prevents any fish from reaching the protected range, changes will be required.

This large protected walleye size range is rare for this region, and will require close scrutiny during 2003.Channel, Blue, and/or Flathead Catfish: Catfish generally were not considered primary shad predators in the lake. Consequently no size restrictions were thought necessary.

At KDWP recommendation, the creel limit was increased from two to five per day in 2000. No adverse impacts were identified and no changes were recommended.

4.0 CONCLUSION

S AND MANAGEMENT IMPLICATIONS Fishery monitoring revealed that gizzard shad catch rates have remained low, with the average size increasing indicating an older population.

To the benefit of WCGS, low shad densities have kept impingement potential at the intake screens low.Predator body conditions remained low, similar to past years, indicating low shad availability.

Average length distributions of most predator species were good with high percentages of larger fish. This was true especially for white bass, white crappie, and walleye. The 1995 through 1998, and 2001 wiper stockings appeared successful, with average size increasing due to aging. Wiper stocking in 2004 is recommended due to the aging of the current year classes.Budgeting for stocking in 2003 is recommended.

No adverse impacts to the fishery from angler harvest were identified.

Due to the restrictive creel and angler limits, few fish were harvested.

Most game fish indices indicated good recruitment, but low body conditions.

This was indicative of a prey-limited fishery, which benefits plant operation by keeping shad numbers low. New innovative size limits for smallmouth bass and walleye were put in place for 2003. Monitoring will determine if the limits succeed in encouraging larger sizes of these predators, or if the limits should be changed.10 5.0 LITERATURE CITED Anderson, R. 0. 1980. Proportional stock density (PSD) and relative weight (Wr): interpretive indices for fish populations and communities.

Pages 27-33 in S. Gloss and B. Shupp, editors. Practical fisheries management:

More with less in the 1980's. New York Chap., Amer. Fish. Soc., Workshop Proceedings.

Boxrucker, J., D. Degan,.D.

DeVries, P. Michaletz, M. J. Van Den Avyle, B. Vondracek.

-1991 (year not specified).

Sampling Shad in Southern Impoundments.

U.S. Fish and Wildlife Service, Reservoir committee of the Southern Division-American Fisheries Society, Coop agreement No. 14-16-0002-91-216.

22 pp.Bruce Nuclear Generating Station. 1977. Fish Impingement at Bruce Nuclear Generating Station. Ontario Hydro Electric Company. 26 pp.Carlander, K. D. 1969. Handbook of Freshwater Fisheries Biology, Vol. 1. Iowa State University Press, Ames, Iowa. 752 pp.Colvin, Mike. 1993. Ecology and management of white bass: a literature review. Missouri Department of Conservation, Dingell-Johnson Project F-1-R-42, Study 1-31, Job 1, Final Report.Colvin, Mike. 1995. Summary of 1994 gill netting at Wolf Creek Reservoir, Kansas. Missouri Department of Conservation.

Jefferson City, MO.Gablehouse, D. W., Jr. 1984. A length-categorization system to assess fish stocks. North American Journal of Fisheries Management.

Vol. 4. P 273-285.Kansas Gas and Electric Company. 1984. Wolf Creek Generating Station 1983 Preoperational Fishery Monitoring Report. Burlington, KS. 82 pp.Nuclear Regulatory Commission.

1982. Final Environmental Statement Related to the Operation of Wolf Creek Generating Station, Unit No. 1, NUREG-0878.

Nuclear Regulatory Commission.

1999. Manual reactor trip from 75% power due to shad blocking the intake screens and the subsequent loss of all circulating water pumps.Event Report from Arkansas Nuclear to NRC. Event No. 35192.Olmstead, L.L. and J.P. Clugston.

1986. Fishery Management in Cooling Impoundments in Reservoir Fisheries Management, Strategies for the 80's. G. Hall and M. Van Den Avyle ed. American Fisheries Society. Bethesda, MD. 327 pp.Ontario Hydro. 1977. Winter studies of gizzard shad at Lambto GS-1976-77.

Ontario Hydro Research Division Report. No. 77-400-K.

47pp.Pflieger, W. L. 1975. The Fishes of Missouri.

Missouri Department of Conservation.

343 pp.Putman, J. H., and D. R. DeVries. 1994. The influences of gizzard shad (Dorosoma cepedianum) on survival and growth of largemouth bass (Micropterus salmoides), bluegill (Lepomis machrochirus), and white crappie (Pomoxis annularis).

Alabama Department of Conservation and Natural Resources.

Investigation of Management Techniques for Public Waters, Study XIV. Federal Aid in Fish Restoration Project F-40-R, Study XIV.1I Stein, R. A. and B. M. Johnson. 1987. Predicting carrying capacities and yields of top predators in Ohio impoundments.

Ohio Department of Natural Resources, Division of Wildlife.

Federal Aid in Fish Restoration Project F-57-R-5 through R-9, Study 12. 144 pP.Wege, G. J. And R. 0. Anderson.

1978. Relative weight (Wr): a new index of condition for largemouth bass. Pages 79-91 in G. D. Novinger and J. G. Dillard, editors. New approaches to the management of small impoundments.

North Central Division, American Fisheries Society. Special Publication 5, Bethesda, MD.White, Andrew M., F. D. Moore, N. A. Alldridge, and D. M. Loucks. 1986. The Effects of Natural Winter Stresses on the Mortality of the Eastern Gizzard Shad, Dorosoma cepedianum, in Lake Erie. Environmental Resource Associates, Inc. and John Carrol University, for The Cleveland Electric Illuminating Company and The Ohio Edison Company. 208 pp.Willis, D.W., K.D. McLoskey and D.W. Gablehouse, Jr. 1985. Calculation of stock density indices based on adjustments for gill net mesh size efficiency.

North American Journal of Fisheries Management.

Vol. 5. P 123-137.Wolf Creek Nuclear Operating Corporation.

1992. Wolf Creek Generating Station, 1991 Operational Fishery Monitoring Report. Burlington, KS. 81 pp.12 Table 1. Fishery sampling effort by gear type used at Wolf Creek Lake during 2002.Water Gear Date (1) Location Effort Temp °F Electrofishina (21 5/9 2 (3)30 68 6/13 6/27 9/24 Fyke Netting (4)10/16 11/7 6 8 9 6 8 2 2 6 8 9 2 6 8 9 2 6 8 9 2 9 6 8 2 9 6 8 6 8 6 8 30 30 30 30 30 30 15 (5)2 2 2 2 2 2 2 2 (7) 1 1 1 1 1 1 1 1 (9)2 2 2 2 30 30 30 63 62 66-74 74-77 75 83 63-69 72 70 72-100 55-63 64-65 61 77-83 49-60 52-53 51 72-75 64-71 79-89 68-69 67-68 74-80 78-92 69 68 63-64 60-62 52-54 50-52 Standard Gill Netting (6)10/8 10/9 10/10 10/11 10/16 11/6 Small Mesh Gill Netting (8)(1)(2)(3)(4)(5)(6)(7)(8)(9)See Figure 1 for locations.

Equipment consisted of a boat-mounted Smith-Root unit operated at 220v, 9-10 amp, DC current pulsed 120 cycles/second Shock effort shown as minutes water was energized.

Fyke net gear consisted of a 4'x5' large frame with either 0.5" or 1" mesh netting.Fyke netting effort listed as number of trap-net-nights set.Standard gill nets consisted of a complement of four 8'xlOO' monofilament nets, one each of 1", 1.5", 2.5", and 4" uniform mesh.Standard gill netting effort listed as number of net-complement-nights set.Small-mesh gill nets consisted of a complement of two 8'xlOO' monofilament nets, one with 0.5", and the second with 0.75" uniform mesh.Small-mesh gill netting effort listed as number of small-mesh-complement-nights set.13 Table 2. Relative abundance and percent biomass of selected fish species in Wolf Creek Lake. Data are from standardized locations and gears expressed as percent of total. Sufficient data was not collected during 2001 due to September 11 events.Species 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 00 02 Gizzard Abundance 19 5 20 21 11 22 27 28 14 34 27 27 39 19 23 12 15 16 Shad Biomass 6 2 5 4 2 4 4 6 4 8 5 9 8 8 8 9 8 6 Common Abundance 1 1 3 3 3 2 2 1 2 3 1 2 2 2 3 3 1 2 Carp Biomass 9 13 16 17 14 12 9 6 11 21 7 11 14 10 12 13 9 8 Smallmouth Abundance

<1 <1 <1 <1 1 <1 <1 1 1 <1 1 <1 1 4 2 2 2 3 buffalo Biomass 1 1 1 5 7 1 3 6 5 5 5 2 3 16 9 8 11 12 Channel Abundance 1 1 3 2 3 2 4 3 4 5 5 6 2 4 9 7 5 10 catfish Biomass 9 6 12 7 9 8 15 6 9 13 10 10 6 6 13 11 10 14 White bass Abundance 6 2 5 4 5 8 10 14 17 15 20 17 8 23 23 22 25 27 Biomass 8 5 7 6 6 12 8 15 19 12 26 17 5 12 13 13 12 15 Wiper Abundance 1 3 3 3 4 5 4 5 2 1 2 4 2 4 3 2 2 3 Biomass 9 21 14 14 17 21 14 17 7 6 11 13 5 8 7 5 7 8 Bluegill Abundance 17 27 30 23 27 22 12 11 11 10 9 4 5 3 5 11 9 4 Biomass 3 2 2 1 1 1 1 1 1 1 1 <1 1 <1 <1 1 1 1 Smallmouth Abundance

<1 1 1 3 4 5 5 7 7 8 8 6 6 7 7 6 5 4 Bass Biomass 1 1 2 3 2 4 6 6 5 5 4 5 7 6 -6 6 6 5 Largemouth Abundance 4 14 11 7 8 9 6 5 5 3 3 1 1 1 1 1 1 1 Bass Biomass 14 17 19 12 11 14 9 5 6 3 3 1 1 <1 1 1 1 1 14 Table 2. (cont.)Species White A crappie B Black A crappie B Walleye A B Freshwater A drum B bundance iomass bundance iomass bundance*iomass bundance iomass 84 85 2 2 2 2 8 10 6 7 3 4 10 12<1 <1 1 1 86 87 88 89 90 91 92 93 94 95 96 97 98 99 00 02 3 6 7 4 5 5 6 7 6 8 8 6 6 11 13 11 4 9 9 4 7 5 7 7 5 7 9 6 5 9 7 11 4 5 3 3 2 4 2 1 1 1 <1 <1 <1 1 <1 2 3 6 3 3 3 3 2 1 1 1 <1 <1 <1 1 <1 2 5 4 7 6 8 5 9 5 9 11 14 14 13 11 11 9 11 9 13 12 17 11 17 12 15 16 25 18 17 13 17 10 1 1 2 1 3 1 2 1 1 2 3 4 3 5 5 4 2 2 1 1 3 1 2 1 1 2 3 4 4 5 5 2 15 Table 3. Catch-per-unit-of-effort (CPUE) of selected fish species in Wolf Creek Lake. Fall gill net, Fyke net, and electrofishing data were not collected in 2001 due to the September 11 events.Gizzard Smallmouth Largemouth White Shad White bass Wiper Bluegill Bass Bass Crappie Walleye 1983 (1)7 (1) 23 (1) 15 (2) 8.0 (2) 24.5 (4) 0 (1) 4 1984 25 18 11 27.0 45.0 6 29 1985 3 6 22 34.3 45.3 5 26 1986 32 25 14 17.3 (3) 1.3 34.5 5 9 1987 10 18 21 8.0 8.5 18.8 12 16 1988 12 28 26 12.0 10.5 22.0 9 19 1989 18 17 23 9.0 14.8 32.3 4 22 1990 10 34 12 1.5 12.0 14.0 5 13 1991 14 45 22 6.7 20.5 5.5 4 19 1992 19 17 9 7.0 10.8 8.3 6 22 1993 11 52 8 7.0 15.0 5.0 5 12 1994 9 61 11 3.0 12.5 2.0 4 23 1995 25 29 11 2.5 6.3 2.0 5 16 1996 9 19 3 1.7 10.8 0.3 9 20 1997 19 60 8 2.3 5.5 1.3 4 28 1998 18 45 6 4.0 10.5 1.5 3 16 1999 15 37 4 2.3 11 3.3 6 14 2000 18 36 13 7.5 21.5 3.0 (5)9 28 2001 --4.3 -2.0 --2002 11 32 4 4.7 2.0 1.0 6 8 (1) Data from fall standard gill netting. Units equal number per gill-net-complement-night

> stock size.(2) Data from spring electrofishing.

Units equal number per hour shocked > stock size (3) Data from fall electrofishing.

Units equal number per hour shocked > stock size.(4) Data from spring Fyke netting. Units equal number per trap-net-night

> stock size.(5) Data beginning in 2000 were from fall Fyke netting. Units equal number per trap-net-night

> stock size.16 Table 4. Proportional Stock Density (PSD) and Relative Stock Density (RSD) for selected fish species at Wolf Creek Lake.Stock (S), quality (Q), preferred (P), memorable (M), and trophy (T) size ranges are per Gablehouse (1984). .Fall gill net, Fyke net, and electrofishing data were not collected in 2001 due to the September 11 events.Species Index 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 Gizzard PSD 24 31 84 92 96 97 10 92 93 98 51 75 96 94 99 97 67 -96 0 shad (1)(2) RSD-P 76 69 16 8 4 3 0 8 7 2 49 25 4 6 1 3 33 -4 White PSD 34 92 74 82 35 63 82 43 85 76 58 46 61 47 66 -55 bass (1)(2) RSD S-Q 66 10 26 18 66 37 17 60 15 24 42 54 39 53 34 -45 RSD Q-P 12 11 12 10 4 10 33 9 7 3 55 2 6 2 2 -7 RSD P-M 18 40 32 57 23 37 40 32 56 59 0 44 49 43 56 -43 RSD M-T 5 39 28 18 6 19 10 2 22 14 3 < 1 4 3 8 -5 RSD T+ < 1 1 Wiper(1) PSD 10 10 10 10 10 97 96 10 10 10 10 85 30 88 89 10 10 -10 0 0 0 0 0 0 0 0 0 0 0 0 RSD S-Q 3 4 15 70 12 11 -RSD Q-P 1 10 14 3 32 11 -24 RSD P-M 78 98 90 55 42 40 28 47 39 21 6 4 33 73 91 58 -31 RSD M-T 29 7 9 45 58 50 53 53 61 76 92 81 30 23 5 9 42 -45 RSD T+ 1 1 2 -Bluegil13)

PSD 68 34 55 34 44 22 0 30 25 21 17 30 14 0 50 33 31 6 32 RSD S-Q 32 68 45 66 56 78 10 70 75 79 83 70 86 10 50 67 69 94 68 0 0 RSD Q-P 54 42 19 30 21 21 17 30 14 25 33 31 6 21 RSD P-M 1 2 3 4 13 11 RSD M-T RSD T+Smallmouth PSD 55 29 37 40 61 40 44 40 52 58 50 52 77 70 -88 Bass(4) RSD S-Q 45 71 63 60 39 60 56 60 48 42 50 48 23 30 -13 RSD Q-P 26 8 25 10 22 26 17 20 28 28 23 29 34 28 -38 RSD P-M 28 17 10 27 32 13 20 12 20 26 18 21 36 40 -50 RSD M-T 4 5 4 6 1 7 8 4 5 9 2 7 2 -RSD T+ 1 17 18 Table 4. (cont.)Species Index 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 Largemouth PSD 76 92 91 93 92 99 97 10 82 85 88 10 10 60 50 10 10 88 50 0 0 0 0 0 Bass (5) RSD S-Q 24 8 9 7 8 1 3 18 15 12 40 50 50 13 25 RSD Q-P 39 62 63 32 19 28 19 5 12 10 13 13 20 17 50 38 25 RSD P-M 32 32 30 60 72 71 80 95 71 71 75 88 10 40 33 10 50 50 0 0 RSD M-T RSD T+White PSD 10 10 97 99 10 10 10 10 95 10 10 99 10 10 10 82 -98 0 0 0 0 0 0 0 0 0 0 0 crappie (6) RSD S-Q 3 1 5 1 18 -2 RSD Q-P 13 13 12 2 12 9 3 3 2 8 1 9 9 9 43 -34 RSD P-M 68 37 19 4 10 13 7 26 14 44 11 12 15 12 13 11 -11 RSD M-T 20 52 68 85 60 70 87 63 75 41 87 72 71 74 77 28 -52 RSD T+ 10 21 10 3 8 4 7 1 6 5 5 1 1 -1 Walleye 1) PSD 76 76 75 10 94 93 96 77 93 90 52 83 73 31 55 74 78 -47 0 RSD S-Q 24 24 25 6 7 4 23 7 10 48 17 27 69 45 26 22 -53 RSD Q-P 77 76 74 92 81 80 95 59 74 67 41 82 67 28 51 74 75 -40 RSD P-M 2 3 3 10 14 13 1 18 19 22 10 1 6 3 4 3 -8 RSD M-T RSD T+(1) Data from fall gill netting.(2) Corrected for gill net efficiency (Willis et al 1985)(3) Data from spring electrofishing.

(4) Data from fall electrofishing.

(5) Data from spring Fyke netting.(6) Data from spring Fyke netting 1999 and earlier, from fall Fyke netting 2000 and later.19 Table 5. Relative weight (Wr) of selected fish species in Wolf Creek Lake. Wr formulas from KDWP were used. Per Wege and Anderson (1978), Wr values of 100 and higher represent fish at or above the 75 percentile, values of 93 to 100 are between the 50 and 75 percentile, values of 86 to 93 are between the 25 and 50 percentile, and values less than 86 are below the 25 percentile.

Fall gill net, Fyke net, and electrofishing data were not collected in 2001 due to the September 11 events.Gizzard Smallmouth Largemouth White Shad White bass Wiper Bluegill Bass Bass Crappie Walleye 1983 (1) 85 (1) 78 (1) 90 (2) 107 (2) 97(4) 107 (1) 78 1984 87 94 86 103 98 93 82 1985 88 89 78 102 97 94 83 1986. 85 86 84 111 93 93 81 1987 89 93 89 105 (3) 97 88 89 80 1988 90 94 85 108 92 92 102 81 1989 104 95 80 96 92 87 88 88 1990 100 99 82 121 104 84 98 85 1991 93 93 78 111 91 79 99 86 1992 93 92 88 102 91 84 95 86 1993 93 94 88 92 91 80 85 85 1994 93 90 75 104 86 75 97 85 1995 88 97 88 124 90 89 105 85 1996 89 106 100 121 100 57 104 94 1997 89 97 89 105 81 90 99 88 1998 81 90 83 83 86 91 95 76 1999 82 93 83 105 90 78 97 81 2000 76 86 77 106 85 78 (5)88 80 2001 --102 2002 87 88 75 110 82 89 (5) 95 77 (1) Data from fall gill netting.(2) Data from spring electrofishing.

(3) Data from fall electrofishing.

(4) Data from spring Fyke netting.(5) Data from fall Fyke netting.20 Table 6. Fish released and harvested by anglers at Wolf Creek Lake. Values expressed as fish per angler hour. The lake was closed to public access from September 2001 through March 2002 due to the events of September 11, 2001, and to boat fishing from November through December 2002 due to low water level.# Total Chan. White Wiper Smallmo. Largemo.Anglers # fish catfish bass hybrids Bass Bass Crappie Walleye All fish Caught and released 1999 9008 86,464 0.15 0.32 0.07 0.37 0.08 0.15 0.65 1.82 2000 6865 61,102 0.15 0.23 0.07 0.36 0.14 0.16 0.63 1.77 2001 7449 60,417 0.16 0.25 0.05 0.28 0.13 0.21 0.59 1.70 2002 4227 34,807 0.19 0.17 0.08 0.38 0.04 0.22 0.56 1.65 Harvested 1999 9008 6007 0.03 0.02 <0.01 0.01 <0.01 0.01 0.03 0.13 2000 6865 4366 0.07 0.02 <0.01 0.01 <0.01 0.01 0.01 0.13 2001 7449 6291 0.08 0.03 <0.01 0.01 <0.01 0.01 0.05 0.18 2002 4227 3841 0.08 0.02 <0.01 0.01 <0.01 0.01 0.06 0.18 21 N III Main Lake Area 6 Figure 1. Fishery sampling location on Wolf Creek Lake.22 Gizzard Shad CPUE Wolf Creek Lake CL CD 00 04 A aE E 0 100 90 80 70 60 50 40 30 20 10 0 I 100 90 80 70 60 50 40 30 20 10 0 Cu E w. E-0 0 Figure 2. Gizzard shad catch-per-unit-effort (CPUE) from October small-mesh gill net complements and standard mesh gill net complements.

Data was not collected during 2001 due to the events of September 11.23

-~4.7"\J WOLF CREEK GENERATING STATION Wolf Creek Lake 2003 FISHERY MONITORING REPORT and 2004 PLAN Prepared by: Supervisor Regulatory Support Approval: Manager Regulatory Affairs Approval: 3/3/04 Dan Haines FQl ) 3/4/04 Bob Hammond Date 3/12/04 Kevin Moles Date EXECUTIVE

SUMMARY

The results obtained from fishery monitoring of Wolf Creek Lake (WCL) during 2004 indicate that the potential for gizzard shad impingement at the cooling water intake screens has remained low. The primary objective of the monitoring was to measure fish population dynamics to determine shad impingement potential.

The fishery assessments targeted gizzard shad, the predator species that feed on them, and the predator-prey interactions.

Catch frequencies of young gizzard shad remained low during 2003. Consequently, no impingement problems developed.

No signs of increasing densities of shad were observed.The 2003 monitoring revealed that the predator populations showed favorable signs for continued shad control. Predator populations, as a whole, showed signs of being prey limited.Growth rates and body conditions improved for most species. Continuous declines in these areas would raise concerns, because it is important that the predator populations remain viable so that shad control continues.

Catch rates were similar to past years', and recruitment was evident for many predator species. Predator populations assessed were white bass, wiper hybrids, largemouth bass, smallmouth bass, white crappie, and walleye.Angling impacts to the predators' shad control benefits were also assessed.

The catch-and-release philosophy being stressed at WCL has made the limited harvest compatible with continued shad control. Innovative length limits were put in place for smallmouth bass and walleye in an attempt to promote larger individuals.

Significant improvements that could be tied to the changes were not present, except possibly increases in body conditions.

The potential for supporting larger individuals with the length limits used is encouraging.

Monitoring data will be important to ensure no adverse impacts to the fishery results from angler harvest.2 TABLE OF CONTENTS EXECUTIVE SUM MARY ..............................................................................................................

2 1.0 2002 FISHERY MONITORING REPO RT .........................................................................

5 1.1 INTRO DUCTIO N ....................................................................................................

5 1.2 M ETHO DS ....................................................................................................................

5 1.3 RESULTS AND DISCUSSION

...............................................................................

6 1.3.1 PREDATOR/PREY INTERACTIONS

...........................................................

6 1.3.1.1 Gizzard Shad ....................................................................................

6 1.3.1.2 Predators

...........................................................................................

7 1.3.2 ANGLER HARVEST IM PACTS ...................................................................

8

1.4 CONCLUSION

S AND MANAGMENT IMPLICATIONS

..........................................

10 2.0 2004 FISHERY MONITORING PLAN .............................................................................

12 2.1 BASIS FOR PLAN .................................................................................................

12 2.2 M ETHO DS ..................................................................................................................

12 2.2.1 SCHEDULE AND LOCATIONS

....................................................................

12 2.2.2 G EAR TYPES ............................................................................................

13 2.2.2.1 Electrofishing

..................................................................................

13 2.2.2.2 Gill Netting ......................................................................................

13 2.2.2.3 Fyke Netting ....................................................................................

14 2.2.2.4 Creel Census ..................................................................................

14 2.2.3 AGE DETERM INATION AND CALIBRATION

............................................

14 2.2.4 REPO RTING ..................................................................................................

14 3.0 LITERATURE CITED .............................................................................................

16 3 List of Tables Table 1. Fishery sampling effort by gear type used at Wolf Creek Lake during 2003 ...... 19 2. Catch-per-unit-effort of Selected Fish Species in Wolf Creek Lake .................

20 3. Proportional Stock Density and Relative Stock Density for Selected Fish Species in W olf Creek Lake ................................................................

21 4. Relative Weight of Selected Fish Species in Wolf Creek Lake ...................

23 5. Fish Released and Harvested By Angler at Wolf Creek Lake .....................

24 6. Fish Sampling Schedule at WCGS during 2004 .......................................

25 List of Figures Figure 1. Fishery sampling locations on Wolf Creek Lake .....................................

26 2. Gizzard shad catch-per-unit-of-effort (CPUE) from October small-mesh gill net complements and standard mesh gill net complements

............................

27 4 1.0 2003 FISHERY MONITORING REPORT

1.1 INTRODUCTION

This report presents and interprets the results of fishery monitoring activities on Wolf Creek Lake (WCL), and demonstrates that the fishery has functioned as desired through 2003. The goal is to increase public safety and plant operating efficiency by reducing the potential for excessive gizzard shad young-of-year (YOY) impingement on the Circulating Water System intake screens. Just such impingement events typically cause plants, with similar cooling water intakes as at WCGS, to either reduce power or shut down. When this occurs at coal plants, like LaCygne Power Station, little attention is given outside the plant operations organization.

At WCGS, such a shad impingement event would likely raise media, as well as regulatory attention.

These shad impingement events typically occur during winter, when demand for electricity is high. Consequently, the returns for reducing the potential for shad impingement at WCGS, by proactively managing the fishery are great. Shad impingement problems to date have been nonexistent, because of the current fishery.Typical impingement problems on intake screens develop because gizzard shad cannot avoid intake flows when they naturally become weakened, and eventually die, as winter water temperatures fall below approximately 400 F (Bruce NGS 1977, Ontario Hydro 1977, Olmstead and Clugston 1986, White et al 1986). Arkansas Nuclear recently experienced such problems with excessive threadfin shad impingement during a winter die-off in late 1998 (NRC 1999; C.Adams, Arkansas Nuclear, personal communication).

Early during WCL construction, it was expected that shad could not be excluded from, and would flourish in the lake. Consequently, an aggressive stocking program was completed (KG&E 1984), and the resulting fishery has not caused impingement problems at WCGS Circulating Water Screen House.Public use of the fishery is also important to maintain community relations and local economic benefits.

Consequently, maintaining and/or enhancing public enjoyment of the fishery that is compatible with the shad impingement control is another important goal of this program. Creel and length limits were determined jointly with the Kansas Department of Wildlife and Parks (KDWP). The catch-and-release strategy employed appears to have succeeded with no detrimental changes to the fishery observed through 2003. No fish stocking in 2003 was recommended.

1.2 METHODS The methods employed during 2003 allowed for continued analyses of important long-term trends. Electrofishing, trap (Fyke) netting, and gill netting were used at long-term sites on WCL (Figure 1). Small-mesh gill netting replaced shoreline seining in 1998 to better assess young-of-year (YOY) gizzard shad densities and recruitment (Boxrucker et al -1991). Important species to the fishery were targeted when expected to be efficiently sampled.Sampling efforts are listed in Table 1. Fish sampled were weighed to the nearest gram, and measured (total length, TL) to the nearest millimeter.

Proportional stock density (PSD, Anderson 1980), incremental relative stock density (RSD, Gablehouse 1984), and relative weight (Wr, Wege and Anderson 1978) were indices applied. Length-weight equations adopted by KDWP were used. Gill net efficiency adjustments to the PSD and RSD indices were completed for gizzard shad, white bass, and walleye (Willis et al 1985).5 I 1.3 RESULTS AND DISCUSSION 1.3.1 PREDATOR/PREY INTERACTIONS The fishery in Wolf Creek Lake exhibited signs of low prey densities.

Typical prey species, such as gizzard shad, tend to produce a large number of young each year.Characteristics of an annually cropped prey population, such as in WCL, would be a high percentage of larger, older individuals, fast growth of YOY, and good health of individuals.

The number of fish making it to reproductive age (recruitment) would also be low. A concern with excessive cropping would be if the number of reproducing adults became too low to produce enough young to support the predators.

This would result in a subsequent loss of the predators.

Characteristics of predator populations in a low-prey fishery would include low recruitment due to cannibalism or predation, slow or no growth of adults, large percentages of older individuals, and poor health of adults. Difficulty in producing trophy size individuals would also be evident.1.3.1.1 Gizzard shad The potential for excessive gizzard shad impingement remained small due to relatively low YOY densities going into the winter months. The shad appear to be limited by predation, as indicated by the population indices of the predator species.Gizzard shad typically has been an important forage species in most reservoirs (Carlander 1969, Pflieger 1975, Stein and Johnson 1987, Colvin 1993). For shad to be compatible with WCGS operation, low YOY shad densities must be maintained.

Periodic recruitment of shad young to reproducing adults also must occur to maintain the predators, which in turn control shad numbers. These conditions currently exist in WCL, and benefit WCGS.Gizzard Shad YOY Densities and Recruitment:

Catch-per-unit-effort (CPUE) for the small-mesh gill nets remained low at two per net complement (Figure 2). Numbers of stock size shad captured in the standard mesh gill nets remained stable (Table 2). The PSD index of 74 (Table 3) indicates more recruitment in 2003 than 2002. Periodic recruitment like in 2003 appears normal in the lake and sufficient to maintain shad densities over time.Length frequencies of shad sampled by the small-mesh nets showed YOY shad less than 120 mm, which are sizes most vulnerable to winter die-off and intake impingement (White et al 1986). In WCL, not all of the YOY gizzard shad were sizes considered vulnerable to impingement.

October sampled YOY in WCL typically ranged from 90 to 230 mm (4 to 9 inches). First year shad growth to lengths between 90 and 150 mm were normal. In some instances, WCL lengths up to 230 mm were identified.

These demonstrate growth rates that are considered among the faster growing rates for shad in the United States (Carlander 1969). Earlier than normal spawning in the heated discharge area of the lake likely contributes to the high YOY growth rate in WCL (WCNOC 1992).To confirm growth of YOY shad up to 230 mm, back-calculated lengths determined from scale samples collected from large adults during the October, 1998 gill netting showed that the majority of WCL shad formed their first scale annuli when over 200 6 mm. Similar back-calculated lengths from WCL scale samples were present for 12 large adult shad, which showed first year growth from 126 to 228 mm with an average of 174 mm (Colvin 1995). First scale annuli for shad typically have been during the winter months (Carlander 1969). It was possible shad that spend their first winter in the heated discharge continued to grow throughout the year, and thus did not form first-year annuli. This would explain the high rate of first-year growth from back-calculated scale analyses.Young-of-year gizzard shad characteristically grow quickly to sizes large enough to escape significant predation.

This has been considered a detriment to sport fish management (Putnam and Devries 1994). In WCL, though, the shad's ability to grow too large for the predators likely contributes to the observed long-term balance between shad and predators.

The scale aging analyses discussed above supports this. Many of the reproducing sized shad were recruited from the faster growing YOY shad. These larger, first-year shad were likely spawned earlier than normal, and their growth was enhanced by a longer growing season (NRC 1982, WCNOC 1992). Scale aging analyses also showed that few of the smaller (90-150 mm TL)YOY shad survived to recruit to reproducing size. Heavy predation and mortality due to winter stress were likely causes. Once the larger YOY grew enough to escape the majority of WCL predators, consumption of the smaller shad should have intensified from late summer through early fall. Also, since the shad grew large enough to reduce their susceptibility to winter-kill mortality, their potential to cause plant impingement problems likewise was reduced. Apparently, the faster growing shad avoid predation and winter stress mortality, and comprised the majority of the brood stock. Following this logic, similar maintenance of large reproducing shad densities may not be as likely to occur in the absence of WCGS thermal discharges.

1.3.1.2 Predators Most predators showed improved body conditions in 2003 (Table 4), but signs of being prey limited were also present. This contributes to the reduction of impingement potential at the station's intake screens. Sufficient recruitment has been occurring for all game fish except largemouth bass, which has been low since 1992. Predator densities were similar to 2002, with some increases for white bass and walleye (Table 2).White Bass: A wide range of white bass sizes was sampled in WCL, which was similar to recent years. The lower PSD index indicates higher numbers of stock sized fish possibly representing a dominant year class (Table 3). Higher numbers of these fish contributed to the increased overall density, and body conditions remained similar to previous years (Tables 2 and 4).Wiper: Wiper hybrids appear to be limited in how large they can grow, likely due to current densities of shad. Stockings grew well up to the 510 to 560 mm TL size range (20 -22 inches), but rarely into the trophy size range (Table 3). Their declining body conditions (Table 4) may be indicative of aging fish, as well as low prey availability.

A stocking was recommended for 2004, but, based on higher 2003 sample densities (Table 2), should be postponed pending 2004 gill net results. To maintain wiper presence in the lake, a 2005 stocking may be necessary.

7 Smallmouth bass: The smallmouth bass population was well represented by various size classes with consistent mid-range PSD's (Table 4), indicating good recruitment.

The proportion of larger fish remained similar at good levels in 2003. In addition, average health increased with a Wr value of 88 (Table 4).The CPUE for spring shocked smallmouth bass remained similar to 2002 (Table2).Catch rates for 2002 and 2003 were lower, but within historical ranges. This species is a dominant littoral predator popular with anglers. Consequently, it is recommended that the spring sampling effort be increased to target smallmouth bass.Largemouth bass: Catch frequencies of largemouth bass in WCL have declined greatly since the 1989 high (Table 2). Slight improvement was observed during the spring 2003 electrofishing sampling.

Little confidence should be placed in the population statistics, other than low catch rates, because of the small sample size. Body condition remained average (Table 4). To increase the sample size for largemouth bass, increased spring shocking is recommended.

White crappie: Fyke netting during the fall was used to assess the crappie population since 2000.Spring efforts were used in the past. The fall sample showed high percentages of larger individuals, but also sampled the intermediate sizes not typically caught in the past spring samples (Table 3). Catch rates remained similar to 2002 (Table 2), and average body condition remained excellent (Table 4).Walleye: The walleye population was well represented by individuals from several year classes in the fall gill nets (Table 3). Good recruitment has occurred since 1991, as evidenced by mid-range PSD's (Table 3). Because of the anglers' high preference for walleye and of its value in shad control, catch frequencies should continue to be monitored through 2004. Body conditions improved from poor to good since 2002 indicating better prey conditions.

Higher catch densities (Table 2) indicates that intra-specific competition did not decline, thus promoting higher growth and condition of remaining fish. Variation of shad availability, or other prey, likely was responsible.

1.3.2 ANGLER HARVEST IMPACTS No detrimental impacts due to angler harvest of the predators populations controlling gizzard shad have been observed.

When the lake was opened to public fishing on October 1, 1996, low creel and high length limits were established to ensure no angler impacts to the fishery. Leading up to that time, gizzard shad were showing signs of increased recruitment (Table 3), indicating potential rises in shad production and thus in impingement rates. This did not materialize, with subsequent monitoring showing both low shad recruitment and low body conditions of predators.

Consequently, it was determined that a harvest increase was possible for some species with little potential to increase impingement rates. Modifications to the limits have occurred in the past. No changes are recommended for 2004.8 White bass: A daily creel limit of five white bass > 12 inches was allowed through 2003. Length frequencies and CPUE show continued recruitment, indicating that creel and length limits had no adverse impacts. Angler harvest typically does not impact white bass due to their high recruitment rates and shorter life span. Consequently, the creel limit of five fish per day was modified to unlimited starting in 2003. This matches the KDWP creel for other Kansas lakes. This may have contributed to the increased white bass harvest during 2003 from 0.07 to 0.24 fish per acre (Table 5). The 12-inch minimum size limit was kept to aid anglers in discerning the similar looking wiper hybrids.Wiper: Wipers have been very important for controlling shad and the optimum size based on historic length frequency distributions has been between 500 and 600 mm (approximately 20-24 inches). Wiper populations were not self-sustaining, consequently WCGS has invested and plans to continue investing in replacement wiper stockings, on an as needed basis. The 24-inch length limit for angler harvest was set to protect the investment and to help ensure that the wipers will reach the preferred size range for controlling shad. Wipers larger than 24 inches have generally been older individuals that were not expected to survive much longer, and thus their removal would not impact shad control benefits.

The optimum size could change if higher numbers of shad increase wiper growth, thus exposing higher numbers to harvest. Angler catch and release continued to rise, likely due to the 2001 wiper class becoming larger, and thus more susceptible to capture (Table 5). The harvest rate still remained low. Neverthless, the current wiper creel limit of one per day, and minimum length limit of > 24 inches will remain unchanged.

Smallmouth bass: Smallmouth bass were the dominant shoreline predator and were abundant along the riprap. Good recruitment, coupled with low body conditions in 1999 and 2000 indicated room for a higher harvest rate. Consequently, in 1999 the smallmouth bass length and creel limits were assessed and changed from one > 18 inches and one < 13, or two < 13 inches, to two fish per day, either < 13 or > 16 inches. These changes reflected KDWP's desire to have WCL continue to develop into a trophy smallmouth bass fishery. No detrimental impacts were identified as catch and release rates remained high and harvest remained unchanged (Table 5).Because of the high angler catch rate and continued low body condition (Table 5), it was concluded in 2003 that competition by smallmouth bass for available resources was high in WCL. There was also the desire by KDWP to continue to promote larger bass.Consequently, the size limit for 2003 was changed to two fish, either < 16 or > 20 inches.This was to promote harvest of smaller fish and protect larger sizes. This has encouraged increased harvest of smallmouth bass smaller than the protected slot by 2.3 times (Table 5). These were likely the 13 to 16 inch fish previously protected.

Higher body conditions were observed in the electrofishing samples, and fall PSD-RSD's indicate recruitment still occurred.

No detrimental impacts were obvious. However, because of this species importance to the fishery, it is recommended that spring electrofishing effort be increased to ensure adequate data to assess impacts.9 Largemouth bass: Largemouth bass catch rates remained low through 2002. Setting the length limit at 21 inches was to allow essentially no harvest. No changes to the current length and creel limits were recommended.

White or Black Crappie: The population was characterized by consistent, high percentages of larger individuals (PSD typically

> 90, Table 3), and good to excellent condition indices (Table 4).Occasional dominant year classes common of many crappie populations also appear to be lacking. These conditions indicate low intraspecific competition, which was unusual and likely caused by high mortality of young crappie, possibly through predation.

Harvest of crappie < 14 inches may jeopardize current production and subsequent recruitment, and thus the population's contribution to gizzard shad consumption.

Consequently, the current crappie creel of two per day with minimum lengths of > 14 inches, will remain unchanged.

Walleye: Length frequencies indicate good recruitment of walleye since 1991 (Table 3). Low recruitment of shad, sustained walleye densities, and low body conditions of walleye justified increased harvest. Consequently, the creel and length limits were changed for 2001 from two per day > 18 inches, to two walleye, one of which must be > 18 inches.Angler harvest increased accordingly (Table 5).Continued low body condition indicated continued high competition for prey resources.

In an effort to manage for larger walleye, it was recommended by KDWP and approved by WCNOC to change the size limit for 2003 to two fish, either < 18 or > 26 inches. The theory was that this should increase harvest of smaller walleye, thus give room for remaining fish to grow into the protected size range. Body conditions did improve (Table 4), but other catch indices did not support the "fewer mouths, bigger fish" theory. Harvest rates of walleye less than 18 inches increased only slightly in 2003 (Table 5). Gill net catch rates increased indicating higher densities, and the proportion of stock (10" to 15")and quality (15" to 20") sized fish remained high. It was likely the recruitment and growth masked any detectable influences from increased harvest of under 18 inch walleye. 2003 was just the first season for the higher slot limit. If future harvest pressure prevents any fish from reaching the protected range, changes will be required.

This high of a slot limit is rare for this region, and will require close scrutiny during 2004. The potential for the limit to produce larger and healthier walleye is encouraging.

Channel, Blue, and/or Flathead Catfish: Catfish generally were not considered primary shad predators in the lake. Consequently no size restrictions were thought necessary.

At KDWP recommendation, the creel limit was increased from two to five per day in 2000. No adverse impacts were identified and no changes were recommended.

1.4 CONCLUSION

S AND MANAGEMENT IMPLICATIONS Fishery monitoring revealed that gizzard shad catch rates have remained low, with the average size increasing indicating an older population.

To the benefit of WCGS, low shad densities have kept impingement potential at the intake screens low.10 Predator body conditions remained similar or improved compared to past years, except for wiper hybrids. Average length distributions of most predator species were good with high percentages of larger fish. This was true especially for white bass, white crappie, and walleye.The 1995 through 1998, and 2001 wiper stockings appeared successful, with average size increasing due to aging. Wiper stocking in 2005, based on 2004 gill net results, may be necessary.

Budgeting for stocking in 2005 is recommended.

No adverse impacts to the fishery from angler harvest were identified.

Due to the restrictive creel and angler limits, few fish were harvested.

Most game fish indices indicated good recruitment and improving body conditions indicative of a prey-limited fishery, which benefits plant operation by keeping shad numbers low. New innovative size limits for smallmouth bass and walleye were put in place for 2003, and had no detrimental impacts observed.

Further monitoring will determine if the limits succeed in encouraging larger sizes of these predators, or if the limits should be changed. It is recommended that the spring shocking effort target smallmouth and largemouth bass, rather than past standardized locations to better assess impacts.I!

2.0 2004 FISHERY MONITORING PLAN 2.1 BASIS FOR PLAN The purpose of the monitoring program is to provide the Environmental Management group with information regarding the WCL fishery. This plan complies with group procedure Al 07A-013,"Ecological Monitoring Program Administration".

A variety of sampling gears will be used to assess the condition of adult and juvenile classes of both prey and predator species to provide information on potential impingement impacts to station operation.

In addition, the methods employed will assess the effects of station operation and angler harvest on the fish populations in WCL.There are three targeted issues that the fishery monitoring efforts in this plan have been streamlined to address. They are; (1) YOY gizzard shad changes, (2) adult shad and predator fish population dynamics, and (3) angler harvest impacts to the fishery.First, knowledge of YOY shad production is important because these fish pose the most immediate impingement threat to plant operations.

Identifying increases in YOY numbers before winter temperatures make them vulnerable to impingement will allow operational preparations to compensate for the increased risk of impingement.

These data will be collected from the small-mesh gill netting performed in the fall.Second, the characteristics of the adult fish population provide long-term data to evaluate if YOY shad control benefits will continue.

Higher numbers of shad growing to reproductive size is an indication that less predation is occurring.

Likewise, fewer predator fish growing to reproductive size would indicate declining shad control capabilities.

Increased predator fish health would also indicate this. Stocking recommendations also are derived from the adult fish characteristics.

These data will be collected using standardized sampling techniques at appropriate times of the year. Specific species targeted by each gear type are presented in the METHODS section.Lastly, the adult fishery monitoring will provide information on angler harvest impacts to the fishery. Proper length limit recommendations can be derived from the monitoring data to ensure that public angler harvest and the plant's gizzard shad control efforts remain compatible.

Creel census data collected by Coffey County at the lake access park will be reviewed and compared with the other fishery sampling data.2.2 METHODS 2.2.1 SCHEDULE AND LOCATIONS The 2004 efforts will be completed as scheduled in Table 6. There are four locations identical to past years that will be used for standardized sampling (Figure 1). Spring electrofishing effort will be modified to target smallmouth and largemouth bass habitat by shocking in shoreline transects until a minimum number of fish or a designated length of shore has been sampled 12 2.2.2 GEAR TYPES 2.2.2.1 Electrofishing Electrofishing on WCL will be completed as specified in Table 6. Electrofishing in the spring will provide smallmouth and largemouth bass population characteristics, and data on gizzard shad survival through the previous winter. Bluegill characteristics will also come from the spring shocking effort. These data will be used for catch (CPUE), size (PSD-RSD) and health (Wr) characteristics.

In the past, this effort focused on shocking the same location each time. This provided valuable long-term data for trending, however, because not all locations were located in smallmouth and largemouth bass habitat, it increases sampling variability when focusing on one species. Because enough data has been collected to characterize the long-term trends of the fishery, the effort will target only smallmouth and largemouth bass habitat to reduce sampling variability to better measure changes. This habitat includes riprap, rocky points, and weed beds in 1-8 feet of water during April and May. These habitats will be shocked until a minimum sample size of 100 is obtained, or minimum of 10 percent of estimated available habitat will be shocked. Energized time and shoreline length shocked, using GPS, will be recorded.

Only smallmouth bass, largemouth bass, bluegill, and shad will be netted and weighed and measured.The main components of the shocking unit will be a 3500 watt gasoline generator, a Smith-Root electrofishing control unit, dead-man foot switch and two DC electrode circular arrays. A pulsed DC current of 5-10 amps will be used on all shocking activities.

Appropriate physical parameters and equipment calibration numbers will be recorded on a Fish Data Sheet (attached) or acceptable equivalent.

After being weighed and measured, fish will be released or saved depending on the need to fulfill fish sample requirements for the Radiological/Environmental Monitoring Program. Information concerning electrofishing safety can be found in procedure Al 07A-009.2.2.2.2 Gill Netting Gill netting will be completed as specified in Table 1. The standard gill net effort will provide data for walleye, white bass, wiper hybrid, adult gizzard shad, and white crappie.These data will be used for CPUE, PSD-RSD and Wr.A minimum of eight complement net-nights will be set with a 4 inch, a 2-1/2 inch, a 1-1/2 inch and a 1 inch monofilament gill net comprising each complement.

All nets will be 8'X 100' each. Locations on the lake will be similar to past years. One net complement will be set at each location (2, 6, 8 and 9, Figure 1). A total of 96 man-hours is the personnel resources estimated for the gill net effort. Fish that have been caught in the nets will be removed, measured to the nearest millimeter, weighed, and the survivors will be released.

Radiological environmental samples or voucher specimens may be kept as needed. All appropriate data will be recorded on the Fish Data Sheet (attached) or an acceptable equivalent.

Small mesh gill nets will be set as specified in Table 6. The data will be used to determine CPUE, and length frequency of YOY gizzard shad. Only shad will be counted from these nets. If practical, all shad will be measured, to the nearest mm. For large 13 catches (typically

>100 shad), a representative sample will be measured.

A minimum of eight complement net nights will be set, preferably during the same week as the standard gill nets. A small mesh complement will consist of one net with 1/2 inch mesh, and one net with 3/4 inch mesh. Each will be 8' X 100' monofilament.

A total of 40 man-hours per year is the personnel resources estimated for the small mesh effort. Set locations on WCL will be in Locations 6 (east side only) and 8 (Figure 1). These are areas that are least influenced by the plant's thermal discharges, and where the shad present will have the most potential to impact the plant's circulating water intake screens. Nets will be set in water that is approximately 6 to 12 feet deep. All appropriate data will be recorded on the Fish Data Sheet (attached) or an acceptable equivalent.

2.2.2.3 Fyke Netting A minimum of 16 net-nights will be completed as specified in Table 1. If weather conditions are dangerous, fewer net-nights will be completed.

The Fyke nets will provide data for the crappie, and also gizzard shad production.

These activities generally begin when water temperatures fall to 65-55OF in the fall. Each location will be fished four net-nights each. A total of 54 man-hours is the personnel resources estimated for the Fyke net effort. Fish will be measured to the nearest millimeter, weighed, then released.Radiological environmental samples or voucher specimens may be kept as needed.The appropriate parameters will be recorded on the Fish Data Sheet (attached) or an acceptable equivalent.

These data will be used for CPUE, PSD-RSD and Wr.2.2.2.4 Creel Census Anglers leaving the lake park report the number of fish caught and released, the number kept for personal use, and angler satisfaction.

These creel sheets are collected and tabulated by Coffey County. Data from the census sheets will be used to determine if harvest rates change dramatically and to measure angler success.2.2.3 AGE DETERMINATION AND CALIBRATION Otolith or scale samples may be taken for selected species. The sample envelope will be identified in the field so that species, length, weight, and date can be determined.

Samples will be analyzed for age and growth information as appropriate.

This information will be useful if uncertainty arises in length limit and stocking need recommendations develop.Fish weight and water temperature measurements will be measured using National Bureau of Standards traceable equipment.

Other measurements will use sampler estimates (wind direction, water depth, etc.) or will rely on the manufacturer's internal standardizing procedures or product accuracy (conductivity, measuring boards, etc.). Documentation of appropriate calibration activities will be kept in accordance with procedure Al 07A-014,"Equipment Control and Calibration".

2.2.4 REPORTING A final report detailing the 2004 fishery monitoring activities and results will be compiled.Any trend's influence on the ability of the fishery to control fish impingement events, which may affect WCGS operations, will be identified.

In addition, Operations will be notified, if necessary, of the possibility of increased shad impingement that may be expected during 14 the winter. Any adjustments to angling length and creel limits will be proposed.Recommendations that may include increased monitoring or stocking needs will be presented.

A summary of the fishery monitoring activities will be completed by April 2004, to include in the 2004 Annual Environmental Operating Report, which is mandated by the Environmental Protection Plan, Appendix B to the Facility Operating License.15 3.0 LITERATURE CITED Anderson, R. 0. 1980. Proportional stock density (PSD) and relative weight (Wr): interpretive indices for fish populations and communities.

Pages 27-33 in S. Gloss and B. Shupp, editors. Practical fisheries management:

More with less in the 1980's. New York Chap., Amer. Fish. Soc., Workshop Proceedings.

Boxrucker, J., D. Degan,.D.

DeVries, P. Michaletz, M. J. Van Den Avyle, B. Vondracek.

-1991 (year not specified).

Sampling Shad in Southern Impoundments.

U.S. Fish and Wildlife Service, Reservoir committee of the Southern Division-American Fisheries Society, Coop agreement No. 14-16-0002-91-216.

22 pp.Bruce Nuclear Generating Station. 1977. Fish Impingement at Bruce Nuclear Generating Station. Ontario Hydro Electric Company. 26 pp.Carlander, K. D. 1969. Handbook of Freshwater Fisheries Biology, Vol. 1. Iowa State University Press, Ames, Iowa. 752 pp.Colvin, Mike. 1993. Ecology and management of white bass: a literature review. Missouri Department of Conservation, Dingell-Johnson Project F-I-R-42, Study 1-31, Job 1, Final Report.Colvin, Mike. 1995. Summary of 1994 gill netting at Wolf Creek Reservoir, Kansas. Missouri Department of Conservation.

Jefferson City, MO.Gablehouse, D. W., Jr. 1984. A length-categorization system to assess fish stocks. North American Journal of Fisheries Management.

Vol. 4. P 273-285.Kansas Gas and Electric Company. 1984. Wolf Creek Generating Station 1983 Preoperational Fishery Monitoring Report. Burlington, KS. 82 pp.Nuclear Regulatory Commission.

1982. Final Environmental Statement Related to the Operation of Wolf Creek Generating Station, Unit No. 1, NUREG-0878.

Nuclear Regulatory Commission.

1999. Manual reactor trip from 75% power due to shad blocking the intake screens and the subsequent loss of all circulating water pumps.Event Report from Arkansas Nuclear to NRC. Event No. 35192.Olmstead, L.L. and J.P. Clugston.

1986. Fishery Management in Cooling Impoundments in Reservoir Fisheries Management, Strategies for the 80's. G. Hall and M. Van Den Avyle ed. American Fisheries Society. Bethesda, MD. 327 pp.Ontario Hydro. 1977. Winter studies of gizzard shad at Lambto GS-1976-77.

Ontario Hydro Research Division Report. No. 77-400-K.

47pp.Pflieger, W. L. 1975. The Fishes of Missouri.

Missouri Department of Conservation.

343 pp.Putman, J. H., and D. R. DeVries. 1994. The influences of gizzard shad (Dorosoma cepedianum) on survival and growth of largemouth bass (Micropterus salmoides), bluegill (Lepomis machrochirus), and white crappie (Pomoxis annularis).

Alabama Department of Conservation and Natural Resources.

Investigation of Management Techniques for Public Waters, Study XIV. Federal Aid in Fish Restoration Project F-40-R, Study XIV.16 Stein, R. A. and B. M. Johnson. 1987. Predicting carrying capacities and yields of top predators in Ohio impoundments.

Ohio Department of Natural Resources, Division of Wildlife.

Federal Aid in Fish Restoration Project F-57-R-5 through R-9, Study 12. 144 pp.Wege, G. J. And R. 0. Anderson.

1978. Relative weight (Wr): a new index of condition for largemouth bass. Pages 79-91 in G. D. Novinger and J. G. Dillard, editors. New approaches to the management of small impoundments.

North Central Division, American Fisheries Society. Special Publication 5, Bethesda, MD.White, Andrew M., F. D. Moore, N. A. Alldridge, and D. M. Loucks. 1986. The Effects of Natural Winter Stresses on the Mortality of the Eastern Gizzard Shad, Dorosoma cepedianum, in Lake Erie. Environmental Resource Associates, Inc. and John Carrol University, for The Cleveland Electric Illuminating Company and The Ohio Edison Company. 208 pp.Willis, D.W., K.D. McLoskey and D.W. Gablehouse, Jr. 1985. Calculation of stock density indices based on adjustments for gill net mesh size efficiency.

North American Journal of Fisheries Management.

Vol. 5. P 123-137.Wolf Creek Nuclear Operating Corporation.

1992. Wolf Creek Generating Station, 1991 Operational Fishery Monitoring Report. Burlington, KS. 81 pp.17 Table 1. Fishery sampling effort by gear type used at Wolf Creek Lake during 2003.Water Gear Date (1) Location Effort Temp °F Electrofishing I 5/22 2 (3 30 70 6/3 9/26 11/6 11/7 Fyke Netting (4)10/2 10/3 6 8 2 6 8 6 9 8 2 6 9 2 6 8 2 6 8 9 9 6 8 2 9 6 8 2 9 6 8 6 8 30 30 30 30 30 10/31 11/20 30 15 15 15 30 15 (5)2 2 2 2 2 2 2 2 (7) 1 1 1 1 1 1 1 1 (9)2 2 2 2 67-69 68 65-68 69-70 68-70 68 68 54 56 56-57 58 64-76 68 64 70 67-68 62 59 50-53 66-72 66-68 66 86-89 67-69 68-69 67-69 65-67 56-61 54 53 53 Standard Gill Netting (6)Small Mesh Gill Netting (8)10/6 10/8 10/9 10/20 11/7 11/20 (1)(2)(3)(4)(5)(6)(7)(8)(9)See Figure 1 for locations.

Equipment consisted of a boat-mounted Smith-Root unit operated at 220v, 9-10 amp, DC current pulsed 120 cycles/second Shock effort shown as minutes water was energized.

Fyke net gear consisted of a 4'x5' large frame with either 0.5" or 1" mesh netting.Fyke netting effort listed as number of trap-net-nights set.Standard gill nets consisted of a complement of four 8'xl 00' monofilament nets, one each of 1", 1.5", 2.5", and 4" uniform mesh.Standard gill netting effort listed as number of net-complement-nights set.Small-mesh gill nets consisted of a complement of two 8'xlOO' monofilament nets, one with 0.5", and the second with 0.75" uniform mesh.Small-mesh gill netting effort listed as number of small-mesh-complement-nights set.18 Table 2. Catch-per-unit-of-effort (CPUE) of selected fish species in Wolf Creek Lake. Fall gill net, Fyke net, and electrofishing data were not collected in 2001 due to the September 11 events.Gizzard Smallmouth Largemouth White Shad White bass WiDer Blueuill Bass Bass CranDie Walleve Walleve 110014 M 7 (1) ')'Z (1) 1 r, (2) Q n (2) 1) A r (3) n (1) A 1984 1985 1986 1987 1988 1989 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 25 3 32 10 12 18 10 14 19 11 9 25 9 19 18 15 18 18 6 25 18 28 17 34 45 17 52 61 29 19 60 45 37 36 11 22 14 21 26 23 12 22 9 8 11 11 3 8 6 4 13 27.0 34.3 17.3 8.0 12.0 9.0 1.5 6.7 7.0 7.0 3.0 2.5 1.7 2.3 4.0 2.3 7.5 4.3 (2) 1.3 8.5 10.5 14.8 12.0 20.5 10.8 15.0 12.5 6.3 10.8 5.5 10.5 11 21.5 45.0 45.3 34.5 18.8 22.0 32.3 14.0 5.5 8.3 5.0 2.0 2.0 0.3 1.3 1.5 3.3 3.0 2.0 LJ 6 5 5 29 26 9 12 9 4 5 4 6 5 4 5 9 4 16 19 22 13 19 22 12 23 16 20 28 16 14 28 3 6 (4)9 2002 11 32 4 4.7 2.0 1.0 6 8 2003 10 54 9 2.7 8.0 2.0 7 14 (1) Data from fall standard gill netting. Units equal number per gill-net-complement-night

> stock size.(2) Data from spring electrofishing.

Units equal number per hour shocked > stock size (3) Data from spring Fyke netting. Units equal number per trap-net-night

> stock size.(4) Data beginning in 2000 were from fall Fyke netting. Units equal number per trap-net-night

> stock size.19 Table 3. Proportional Stock Density (PSD) and Relative Stock Density (RSD) for selected fish species at Wolf Creek Lake.Stock (S), quality (Q), preferred (P), memorable (M), and trophy (T) size ranges are per Gablehouse (1984). .Fall gill net, Fyke net, and electrofishing data were not collected in 2001 due to the September 11 events.Species Index 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 Gizzard shad (1)(2)White bass (1)(2)Wiper(l)PSD RSD-P PSD RSD S-Q RSD Q-P RSD P-M RSD M-T RSD T+PSD RSD S-Q RSD Q-P RSD P-M RSD M-T RSD T+PSD RSD S-Q RSD Q-P RSD P-M RSD M-T RSD T+31 84 92 96 97 10 92 93 98 51 75 96 94 99 97 67 0 69 16 8 4 3 0 8 7 2 49 25 4 6 1 3 33 34 92 74 82 35 63 82 43 85 76 58 46 61 47 66 66 10 26 18 66 37 17 60 15 24 42 54 39 53 34 12 11 12 10 4 10 33 9 7 3 55 2 6 2 2 18 40 32 57 23 37 40 32 56 59 0 44 49 43 56 5 39 28 18 6 19 10 2 22 14 3 <1 4 3 8<1 1 10 10 10 10 97 96 10 10 10 10 85 30 88 89 10 10 0 0 0 0 0 0 0 0 0 0 3 4 15 70 12 11 1 10 14 3 32 11 98 90 55 42 40 28 47 39 21 6 4 33 73 91 58 7 9 45 58 50 53 53 61 76 92 81 30 23 5 9 42 1 1 2 55 29 37 40 61 40 44 40 52 58 50 52 77 70 45 71 63 60 39 60 56 60 48 42 50 48 23 30 26 8 25 10 22 26 17 20 28 28 23 29 34 28 28 17 10 27 32 13 20 12 20 26 18 21 36 40 4 5 4 6 1 7 8 4 5 9 2 7 2 1 92 91 93 92 99 97 10 82 85 88 10 10 60 50 10 10 0 0 0 0 0 8 9 7 8 1 3 18 15 12 40 50 50 62 63 32 19 28 19 5 12 10 13 13 20 17 50 32 30 60 72 71 80 95 71 71 75 88 10 40 33 10 0 0-96 74-4 26-55 32-45 68 7 3 43 25 5 3 10 10 0 0 24 31 20 45 80 88 83 13 17 38 17 50 63 4 88 50 10 0 13 25 0 38 25 17 50 50 83 Smallmouth Bass(4)Largemouth PSD Bass (5)RSD S-Q RSD Q-P RSD P-M RSD M-T RSD T+20 21 Table 3. (cont.)Species Index 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 White PSD 10 10 97 99 10 10 10 10 95 10 10 99 10 10 10 82 -98 99 0 0 0 0 0 0 0 0 0 0 0 crappie (6) RSD S-Q 3 1 5 1 18 -2 1 RSD Q-P 13 13 12 2 12 9 3 3 2 8 1 9 9 9 43 -34 48 RSD P-M 68 37 19 4 10 13 7 26 14 44 11 12 15 12 13 11 -11 29 RSD M-T 20 52 68 85 60 70 87 63 75 41 87 72 71 74 77 28 -52 21 RSD T+ 10 21 10 3 8 4 7 1 6 5 5 1 1 -1 1 Walleye 1) PSD 76 75 10 94 93 96 77 93 90 52 83 73 31 55 74 78 -47 60 0 RSD S-Q 24 25 6 7 4 23 7 10 48 17 27 69 45 26 22 -53 40 RSD Q-P 76 74 92 81 80 95 59 74 67 41 82 67 28 51 74 75 -40 57 RSD P-M 3 3 10 14 13 1 18 19 22 10 1 6 3 4 3 -8 3 RSD M-T RSD T+(1) Data from fall gill netting.(2) Corrected for gill net efficiency (Willis et al 1985)(3) Data from spring electrofishing.

(4) Data from fall electrofishing.

(5) Data from spring Fyke netting.(6) Data from spring Fyke netting 1999 and earlier, from fall Fyke netting 2000 and later.22 Table 4. Relative weight (Wr) of selected fish species in Wolf Creek Lake. Wr formulas from KDWP were used. Per Wege and Anderson (1978), Wr values of 100 and higher represent fish at or above the 75 percentile, values of 93 to 100 are between the 50 and 75 percentile, values of 86 to 93 are between the 25 and 50 percentile, and values less than 86 are below the 25 percentile.

Fall gill net, Fyke net, and electrofishing data were not collected in 2001 due to the September 11 events.Gizzard Smallmouth Largemouth White Shad White bass Wiper Bluegill Bass Bass Crappie Walleye 1983 (1) 85 (1) 78 (1) 90 (2) 107 (2) 97 (4) 107 (1) 78 1984 87 94 86 103 98 93 82 1985 88 89 78 102 97 94 83 1986 85 86 84 111 93 93 81 1987 89 93 89 105 (3) 97 88 89 80 1988 90 94 85 108 92 92 102 81 1989 104 95 80 96 92 87 88 88 1990 100 99 82 121 104 84 98 85 1991 93 93 78 111 91 79 99 86 1992 93 92 88 102 91 84 95 86 1993 93 94 88 92 91 80 85 85 1994 93 90 75 104 86 75 97 85 1995 88 97 88 124 90 89 105 85 1996 89 106 100 121 100 57 104 94 1997 89 97 89 105 81 90 99 88 1998 81 90 83 83 86 91 95 76 1999 82 93 83 105 90 78 97 81 2000 76 86 77 106 85 78 (5)88 80 2001 --102 2002 87 88 75 110 82 89 (5) 95 77 2003 85 88 68 116 88 83 96 86 (1) Data from fall gill netting.(2) Data from spring electrofishing.

(3) Data from spring Fyke netting.(4) Data from fall Fyke netting.23 Table 5. Selected fish species caught and released by anglers at Wolf Creek Lake.# Chan. White Wiper Smallmouth All fish Released Anglers catfish bass hybrids Bass LM Bass Crappie Walleye 1999 9008 No. 6928 15,171 3503 17,482 3885 7382 31,027 86,464#/hour 0.15 0.32 0.07 0.37 0.08 0.15 0.65 1.82#/acre 1.36 2.98 0.69 3.43 0.76 1.45 6.10 16.99 2000 6865 No. 5191 7838 2267 12,579 4918 5536 21,599 61,102#/hour 0.15 0.23 0.07 0.36 0.14 0.16 0.63 1.77#/acre 1.02 1.54 0.45 2.47 0.97 1.09 4.24 12.00 2001 7449 No. 5623 8777 1810 10,136 4736 7457 20,911 60,417#/hour 0.16 0.25 0.05 0.28 0.13 0.21 0.59 1.70#/acre 1.10 1.72 0.35 1.99 0.93 1.47 4.11 (11.87)2002 4227 No. 3949 3623 1649 8097 874 4563 11,785 31,807#/hour 0.19 0.17 0.08 0.38 0.04 0.22 0.56 1.65#/acre 0.77 0.71 0.32 1.59 0.17 0.90 2.31 (6.84)2003 4751 No. 6057 8489 6838 8527 3193 5739 6740 45,895#/hour 0.25 0.34 0.27 0.35 0.13 0.23 0.27 1.86#/acre 1.19 1.67 1.34 1.67 0.63 1.13 1.32 (9.02)Harvested

>12" >24" <13" >18" >21" >14" >18" 1999 9008 No. 1628 1149 7 356 116 14 725 1669 6007#/hour 0.03 0.02 <0.01 0.01 <0.01 <0.01 0.01 0.03 0.13#/acre 0.32 0.23 <0.01 0.07 0.02 <0.01 0.14 0.33 1.15 2000 6865 No. 2258 859 3 198 20 10 316 533 4366#/hour 0.07 0.02 <0.01 0.01 <0.01 <0.01 0.01 0.01 1.13#/acre 0.44 0.17 <0.01 0.04 <0.01 <0.01 0.06 0.10 1.35>16" <18" >18" 2001 7449 No. 2779 1046 12 126 69 4 415 1609 36 6291#/hour 0.08 0.03 <0.01 0.01 <0.01 <0.01 0.01 0.05 <0.01 0.18#/acre 0.55 0.21 <0.01 0.02 0.01 <0.01 0.08 0.32 0.01 1.23 2002 4227 No. 1161 378 .7 85 62 7 184 862 326 3841#/hour 0.08 0.02 <0.01 <0.01 <0.01 <0.01 0.01 0.04 0.01 0.18#/acre 0.23 0.07 <0.01 0.02 0.01 <0.01 0.04 0.17 0.06 0.83<16" >20" <18" >26" 2003 4751 No. 2457 1233 16 364 24 1 234 1244 26 5638#/hour 0.10 0.05 <0.01 0.01 <0.01 <0.01 0.01 0.05 <0.01 0.49#/acre 0.48 0.24 <0.01 0.07 <0.01 <0.01 0.05 0.24 <0.01 0.93 24 Table 6. Fish Sampling Schedule at WCGS during 2004.Minimum Information Needed to Assess Fishery Method Preferred Time Frame 1.2.3.4.5.6.7.8.9.Gizzard shad recruitment through winter White crappie population characteristics and health Largemouth bass population characteristics and health Smallmouth bass population characteristics and health White crappie population characteristics and health White bass population characteristics and health Wiper survival and health Walleye population characteristics and health Gizzard shad YOY reproduction and densities going into winter Electrofishing Fyke netting Electrofishing Electrofishing Fyke Netting Gill netting Gill netting Gill netting Small Mesh Gill Netting April/May October/November April/May April/May October October October October September/October 25 N N III Main Lake Area 6 Figure 1. Fishery sampling location on Wolf Creek Lake.26 Gizzard Shad CPUE Wolf Creek Lake CL (n"5 E 0 00 04 A 100 90 80 70 4E 60 50*. 50 3 40 30 20 10 0 100 90 80 70 60 50 40 30 20 10 0 E 4-0 E E 0.0 0 E-oU EN Figure 2. Gizzard shad catch-per-unit-effort (CPUE) from October small-mesh gill net complements and standard mesh gill net complements.

Adult shad (>280mm TL)catches shown with standard mesh nets. Young-of-year shad shown with small mesh net catch. Data was not collected during 2001 due to the events of September 11.27 VS WOLF CREEK GENERATING STATION Wolf Creek Lake 2004 FISHERY MONITORING REPORT AND 2005 PLAN Prepared by: Supervisor Regulatory Support Approval: 3/30/05 Dan Haines Robe 1 4/04/05 Robert Hammond Date 04/06/05 Manager Regulatory Affairs --Approval:

for \Kevin Moles Date I EXECUTIVE

SUMMARY

Monitoring during 2004 demonstrated that the fishery in Wolf Creek Lake remained in good condition with no adverse trends identified.

Fish predation pressure on the gizzard shad population continued to prevent excessive shad impingement problems at the circulating water intake. Fishery monitoring activities in 2005 as outlined in this report will continue to measure long-term trends and help Wolf Creek Generating Station prepare for any short term changes, particularly for any changes in the potential for shad impingement events.Public angling on the lake did not impact the fishery's function of supporting plant operations.

The catch and release philosophy promoted when the lake was opened for the public has been compatible with gizzard shad control objectives.

Monitoring data did warrant management activities to improve the fishery for public use. These recommendations are: 1. Increase of the creel limit for crappie greater than 14 inches from two to ten fish per day to increase angler use and increase harvest of older crappie.2. Increase the catfish creel limit from five to ten fish per day. Catfish are not considered a significant predator of gizzard shad.3. Decrease the wiper length limit from 24 to 21 inches to increase harvest of older fish.4. Budget for a wiper stocking in 2006 to replace current aging year-classes.

2 2004 FISHERY MONITORING REPORT AND 2005 PLAN INTRODUCTION This report presents the results of fishery monitoring activities on Wolf Creek Lake (WCL). Data are summarized in table form to document long-term trends and demonstrates that the fishery has functioned as desired through 2004. The goal is to increase public safety and plant operating efficiency by reducing the potential for excessive gizzard shad young-of-year (YOY) impingement on the Circulating Water System intake screens. Shad impingement problems to date have not occurred due largely to the characteristics of the current fishery.Public use of the fishery is also important to maintain community relations and local economic benefits.

Consequently, maintaining and/or enhancing public enjoyment of the fishery that is compatible with the shad impingement control are other important goals of this program. Creel and length limits were determined jointly with the Kansas Department of Wildlife and Parks (KDWP).The catch-and-release strategy employed appears to have succeeded with no detrimental changes to the fishery observed through 2004.Fishery monitoring activities in 2005 will be similar to 2004 to maintain long-term trending.

Short-term changes will also be detected to ensure WCGS can be prepared if impingement potential increases.

METHODS The monitoring methods used during 2004 allowed for continued analyses of important long-term trends. Gill netting was used at long-term sites on WCL (Figure 1). Spring electrofishing effort targeted smallmouth and largemouth bass habitat by shocking in shoreline transects until a minimum number of fish or a designated length of shore was sampled. Small-mesh gill netting replaced shoreline seining in 1998 to better assess young-of-year (YOY) gizzard shad densities and recruitment (Boxrucker et al -1991). Important species to the fishery were targeted when expected to be efficiently sampled.Sampling efforts are listed in Table 1. Fish sampled were weighed to the nearest gram, and measured (total length, TL) to the nearest millimeter.

Proportional stock density (PSD, Anderson 1980), incremental relative stock density (RSD, Gablehouse 1984), and relative weight (Wr, Wege and Anderson 1978) were indices applied. Length-weight equations adopted by KDWP were used.Gill net efficiency adjustments to the PSD and RSD indices were completed for gizzard shad, white bass, and walleye (Willis et al 1985).The 2005 efforts will be completed as scheduled in Table 2. These efforts are the same as for 2004. Anglers using the lake park report the number of fish caught and released, the number kept for personal use, and angler satisfaction.

These creel sheets are collected and tabulated by Coffey County. Data from the census sheets will be used to determine if harvest rates change dramatically and to measure angler success.RESULTS AND PLANS The fishery in Wolf Creek Lake continued to function as desired. It exhibited signs of low prey densities, which is preferred to minimize fish impingement at the circulating water intake. The potential for excessive gizzard shad impingement remained small due to relatively low YOY densities going into the winter months. The shad appear to be limited by predation, as indicated by the population indices of the predator species. Gizzard shad typically has been an important forage species in most reservoirs (Carlander 1969, Pflieger 1975, Stein and Johnson 1987, Colvin 1993). For shad to be compatible with WCGS operation, low YOY shad densities must be 3 maintained.

Periodic recruitment of shad young to reproducing adults also must occur to maintain the predators, which in turn control shad numbers. These conditions currently exist in WCL, and benefit WCGS.Catch densities of important species remained similar to past years, except for smallmouth bass and bluegill (Table 3). Electrofishing catch rates increased for these species because the shock effort was limited to expected habitats such as rip-rap and weed bed areas, rather than as in the previous years when set locations were shocked that were not necessarily smallmouth or bluegill habitat. The change was made to obtain more accurate data for these species.Fish length frequencies in 2004, as shown by the PSD/RSD indices (Table 4), showed no major changes to past years. Continued recruitment and growth of important species were evident with most showing good percentages of mid-sized individuals (RSDS-Q, RSD Q-P, and RAD M-T size ranges). The high PSD for gizzard shad indicates fewer small sizes recruited, due in part to predation.

This is a long-term trend for shad and indicates stability of the population at current conditions.

For comparison, mid-range PSD's indicated balanced populations for other species.The main exception was for the wiper hybrids. These non-reproducing hybrids had larger percentages in the upper size classes, reflecting maturation of the latest 2001 stocking.

The decrease in the memorable size category for wipers likely is a result of older stockings becoming less abundant due to natural mortality.

Because of this, budgeting for potential wiper stocking in 2006 is recommended.

Body conditions as indicated by Wr indices (Table 5) remained similar to past years. All species showed adequate body conditions to maintain their populations.

Large increases or decreases in body condition were not evident for any species. This indicates that no large changes in prey availability occurred, primarily gizzard shad densities.

No detrimental impacts due to angler harvest of the predator populations controlling gizzard shad have been observed.

Harvest rates were slightly higher, but still similar for most species, except walleye (Table 6 and 7). Harvest of walleye under 18 inches nearly doubled, but was still considered minor. Because the population indices for catch frequency, length frequency, and body conditions remained similar to past years, influence by angler harvest was not apparent.The current smallmouth bass and walleye slot limits were imposed to increase body condition and growth. These limits should remain in effect until more data is collected to assess their impacts.The current minimum length limit (12 inches) for white bass was set to protect younger wipers.Since a wiper year class stocking is possible for 2006, the white bass minimum length should remain in effect. The crappie is an important littoral predator of gizzard shad in the absence of high largemouth densities, so the minimum length limit (14 inches) was set to protect a majority of the larger individuals.

A large proportion of crappie were near the limit (PSD M-T of 47, Table 4), consequently the limit should remain the same.However, some changes in fish harvest regulations are recommended starting in 2006. They are: 1. Increase of the creel limit for crappie greater than 14 inches from two to ten fish per day to increase angler use and increase harvest of older crappie.2. Increase the catfish creel limit from five to ten fish per day. Catfish are not considered a significant predator of gizzard shad.3. Decrease the wiper length limit from 24 to 21 inches to increase harvest of older fish.4 LITERATURE CITED Anderson, R. 0. 1980. Proportional stock density (PSD) and relative weight (Wr): interpretive indices for fish populations and communities.

Pages 27-33 in S. Gloss and B. Shupp, editors. Practical fisheries management:

More with less in the 1980's. New York Chap., Amer. Fish. Soc., Workshop Proceedings.

Boxrucker, J., D. Degan,.D.

DeVries, P. Michaletz, M. J. Van Den Avyle, B. Vondracek.

-1991 (year not specified).

Sampling Shad in Southern Impoundments.

U.S. Fish and Wildlife Service, Reservoir committee of the Southern Division-American Fisheries Society, Coop agreement No. 14-16-0002-91-216.

22 pp.Carlander, K. D. 1969. Handbook of Freshwater Fisheries Biology, Vol. 1. Iowa State University Press, Ames, Iowa. 752 pp.Colvin, Mike. 1993. Ecology and management of white bass: a literature review. Missouri Department of Conservation, Dingell-Johnson Project F-1-R-42, Study 1-31, Job 1, Final Report.Gablehouse, D. W., Jr. 1984. A length-categorization system to assess fish stocks. North American Journal of Fisheries Management.

Vol. 4. P 273-285.Pflieger, W. L. 1975. The Fishes of Missouri.

Missouri Department of Conservation.

343 pp.Stein, R. A. and B. M. Johnson. 1987. Predicting carrying capacities and yields of top predators in Ohio impoundments.

Ohio Department of Natural Resources, Division of Wildlife.

Federal Aid in Fish Restoration Project F-57-R-5 through R-9, Study 12. 144 pp.Wege, G. J. And R. 0. Anderson.

1978. Relative weight (Wr): a new index of condition for largemouth bass. Pages 79-91 in G. D. Novinger and J. G. Dillard, editors. New approaches to the management of small impoundments.

North Central Division, American Fisheries Society. Special Publication 5, Bethesda, MD.Willis, D.W., K.D. McLoskey and D.W. Gablehouse, Jr. 1985. Calculation of stock density indices based on adjustments for gill net mesh size efficiency.

North American Journal of Fisheries Management.

Vol. 5. P 123-137.5 Table 1. Fishery sampling effort by gear type used at Wolf Creek Lake during 2004.Water Gear Date (1) Location Effort Temp 'F Electrofishing I' 4/27 NA (3)2.2 66 6/3 NA 0.4 70 Standard Gill Netting (4) 10/15 2 (5) 1 53-65 9 1 76-80 10/18 6 1 60-63 8 1 60 10/20 2 1 58 9 1 66-81 10/21 6 1 61-62 8 1 59-60 Small Mesh Gill Netting (6) 11/5 6 (7)2 55-58 8 2 55 11/19 6 2 53-54 8 2 53-54 (1) See Figure 1 for locations.

(2) Equipment consisted of a boat-mounted Smith-Root unit operated at 220v, 9-10 amp, DC current pulsed 120 cycles/second (3) Shock effort shown as hours water was energized.

(4) Standard gill nets consisted of a complement of four 8'x100' monofilament nets, one each of 1", 1.5", 2.5", and 4" uniform mesh.(5) Standard gill netting effort listed as number of net-complement-nights set.(6) Small-mesh gill nets consisted of a complement of two 8'x100' monofilament nets, one with 0.5", and the second with 0.75" uniform mesh.(7) Small-mesh gill netting effort listed as number of small-mesh-complement-nights set.6 Table 2. Fish Sampling Schedule at Wolf Creek Lake during 2005.1.2.3.4.5.6.7.8.Minimum Information Needed to Assess Fishery Gizzard shad recruitment through winter White crappie population characteristics and health Largemouth bass population characteristics and health Smallmouth bass population characteristics and health White bass population characteristics and health Wiper survival and health Walleye population characteristics and health Gizzard shad YOY reproduction and densities going into winter Method Preferred Time Frame Electrofishing Fyke netting/Gill netting Electrofishing Electrofishing Gill netting Gill netting Gill netting Small Mesh Gill Netting April/May October/November April/May April/May October October October September/October 7

Table 3. Catch-per-unit-of-effort (CPUE) of selected fish species in Wolf Creek Lake. Fall gill net, Fyke net, data were not collected in 2001 due to the September 11 events.and electrofishing Gizzard Smallmouth Largemouth White Shad White bass Wiper Bluegill Bass Bass Crappie Walleye 1983 (1)7 (1) 23 (1) 15 (2) 8.0 (2) 24.5 (3) 0 (1) 4 1984 25 18 11 27.0 45.0 6 29 1985 3 6 22 34.3 45.3 5 26 1986 32 25 14 17.3 (2) 1.3 34.5 5 9 1987 10 18 21 8.0 8.5 18.8 12 16 1988 12 28 26 12.0 10.5 22.0 9 19 1989 18 17 23 9.0 14.8 32.3 4 22 1990 10 34 12 1.5 12.0 14.0 5 13 1991 14 45 22 6.7 20.5 5.5 4 19 1992 19 17 9 7.0 10.8 8.3 6 22 1993 11 52 8 7.0 15.0 5.0 5 12 1994 9 61 11 3.0 12.5 2.0 4 23 1995 25 29 11 2.5 6.3 2.0 5 16 1996 9 19 3 1.7 10.8 0.3 9 20 1997 19 60 8 2.3 5.5 1.3 4 28 1998 18 45 6 4.0 10.5 1.5 3 16 1999 15 37 4 2.3 11 3.3 6 14 2000 18 36 13 7.5 21.5 3.0 (4)9 28 2001 --4.3 -2.0 --2002 11 32 4 4.7 2.0 1.0 6 8 2003 10 54 9 2.7 8.0 2.0 7 14 2004 12 33 6 20 34 0.8 -20 (1) Data from fall standard gill netting. Units equal number per gill-net-complement-night

> stock size.(2) Data from spring electrofishing.

Units equal number per hour shocked > stock size. Shocking efforts starting in 2004 targeted prime habitats rather than standard locations as completed during prior years.(3) Data from spring Fyke netting. Units equal number per trap-net-night

> stock size. Fyke netting during 2004 was not completed due to adverse weather conditions.

(4) Data beginning in 2000 were from fall Fyke netting. Units equal number per trap-net-night

> stock size.8 Table 4. Proportional Stock Density (PSD) and Relative Stock Density (RSD) for selected fish species at Wolf Creek Lake.Stock (S), quality (Q), preferred (P), memorable (M), and trophy (T) size ranges are per Gablehouse (1984). .Fall gill net, Fyke net, and electrofishing data were not collected in 2001 due to the September 11 events.Species Index 87 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 Gizzard PSD 92 96 97 10 92 93 98 51 75 96 94 99 97 67 -96 74 87 shad (1)(2)0 8 4 3 0 8 7 2 49 25 RSD-P 4 6 1 3 33 White bass (1)(2)Wiper(1)Smallmouth Bass(4)PSD RSD S-Q RSD Q-P RSD P-M RSD M-T RSD T+PSD RSD S-Q RSD Q-P RSD P-M RSD M-T RSD T+PSD RSD S-Q RSD Q-P RSD P-M RSD M-T RSD T+92 74 82 35 63 82 43 85 76 58 46 61 47 66 10 26 18 66 37 17 60 15 24 42 54 39 53 34 11 12 10 4 10 33 9 7 3 55 2 6 2 2 40 32 57 23 37 40 32 56 59 0 44 49 43 56 39 28 18 6 19 10 2 22 14 3 < 1 4 3 8<1 1 10 10 97 96 10 10 10 10 85 30 88 89 10 10 0 0 0 0 0 0 0 0 3 4 15 70 12 11 1 10 14 3 32 11 55 42 40 28 47 39 21 6 4 33 73 91 58 45 58 50 53 53 61 76 92 81 30 23 5 9 42 1 1 2 55 29 37 40 61 40 44 40 52 58 50 52 77 70 45 71 63 60 39 60 56 60 48 42 50 48 23 30 26 8 25 10 22 26 17 20 28 28 23 29 34 28 28 17 10 27 32 13 20 12 20 26 18 21 36 40 4 5 4 6 1 7 8 4 5 9 2 7 2 1 93 92 99 97 10 82 85 88 10 10 60 50 10 10 0 0 0 0 0 7 8 1 3 18 15 12 40 50 50 32 19 28 19 5 12 10 13 13 20 17 50 60 72 71 80 95 71 71 75 88 10 40 33 10 0 0-4 26 20-55 32 53-45 68 47-7 3 6-43 25 41-5 3 6-10 10 10 0 0 0 24 31 20 65 45 80 33 2 88 83 66 13 17 34 38 17 22 50 63 36 4 8 88 50 10 (7)0 13 25 38 25 17 50 50 83 Largemouth PSD Bass (5)RSD S-Q RSD Q-P RSD P-M RSD M-T RSD T+9 10 4.Table 4. (cont.)Species Index 87 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 White PSD 97 99 10 10 10 10 95 10 10 99 10 10 10 82 -98 99 97 0 0 0 0 0 0 0 0 0 crappie (6)(8) RSD S-Q 3 1 5 1 18 -2 1 3 RSD Q-P 12 2 12 9 3 3 2 8 1 9 9 9 43 -34 48 32 RSD P-M 19 4 10 13 7 26 14 44 11 12 15 12 13 11 -11 29 15 RSD M-T 68 85 60 70 87 63 75 41 87 72 71 74 77 28 -52 21 47 RSD T+ 10 21 10 3 8 4 7 1 6 5 5 1 1 -1 1 3 Walleye (1 PSD 10 94 93 96 77 93 90 52 83 73 31 55 74 78 -47 60 69 0 RSD S-Q 6 7 4 23 7 10 48 17 27 69 45 26 22 -53 40 31 RSD Q-P 92 81 80 95 59 74 67 41 82 67 28 51 74 75 -40 57 66 RSD P-M 10 14 13 1 18 19 22 10 1 6 3 4 3 -8 3 3 RSD M-T RSD T+(1) Data from fall gill netting.(2) Corrected for gill net efficiency (Willis et al 1985)(3) Data from spring electrofishing.

(4) Data from fall electrofishing.

(5) Data from spring Fyke netting.(6) Data from spring Fyke netting 1999 and earlier, from fall Fyke netting 2000 and later.(7) Insufficient data to calculate.

(8) 2004 data from fall gill netting.I1 Table 5. Relative weight (Wr) of selected fish species in Wolf Creek Lake. Wr formulas from KDWP were used. Per Wege and Anderson (1978), Wr values of 100 and higher represent fish at or above the 75 percentile, values of 93 to 100 are between the 50 and 75 percentile, values of 86 to 93 are between the 25 and 50 percentile, and values less than 86 are below the 25 percentile.

Fall gill net, Fyke net, and electrofishing data were not collected in 2001 due to the September 11 events.Gizzard Smallmouth Largemouth White Shad White bass Wiper Bluegill Bass Bass Crappie Walleye 1983 (1) 85 (1) 78 (1) 90 (2) 107 (2)97 (4) 107 (1) 78 1984 87 94 86 103 98 93 82 1985 88 89 78 102 97 94 83 1986 85 86 84 111 93 93 81 1987 89 93 89 105 (3) 97 88 89 80 1988 90 94 85 108 92 92 102 81 1989 104 95 80 96 92 87 88 88 1990 100 99 82 121 104 84 98 85 1991 93 93 78 111 91 79 99 86 1992 93 92 88 102 91 84 95 86 1993 93 94 88 92 91 80 85 85 1994 93 90 75 104 86 75 97 85 1995 88 97 88 124 90 89 105 85 1996 89 106 100 121 100 57 104 94 1997 89 97 89 105 81 90 99 88 1998 81 90 83 83 86 91 95 76 1999 82 93 83 105 90 78 97 81 2000 76 86 77 106 85 78 (5)88 80 2001 --102 -84 2002 87 88 75 110 82 89 (5)95 77 2003 85 88 68 116 88 83 96 86 2004 81 87 72 107 84 (5) (1)91 86 (1) Data from fall gill netting.(2) Data from spring electrofishing.

(3) Data from spring Fyke netting.(4) Data from fall Fyke netting.(5) Insufficient sample size to calculate.

12 Table 6. Selected fish species caught and released by anglers at Wolf Creek Lake.# Chan. White Wiper Smallmouth All Anglers catfish bass hybrid Bass LM Bass Crappie Walleye fish 1999 9008 No. 6928 15,171 3503 17,482 3885 7382 31,027 86,464#/hour 0.15 0.32 0.07 0.37 0.08 0.15 0.65 1.82#/acre 1.36 2.98 0.69 3.43 0.76 1.45 6.10 16.99 2000 6865 No. 5191 7838 2267 12,579 4918 5536 21,599 61,102#/hour 0.15 0.23 0.07 0.36 0.14 0.16 0.63 1.77#/acre 1.02 1.54 0.45 2.47 0.97 1.09 4.24 12.00 2001 7449 No. 5623 8777 1810 10,136 4736 7457 20,911 60,417#Ihour 0.16 0.25 0.05 0.28 0.13 0.21 0.59 1.70#/acre 1.10 1.72 0.35 1.99 0.93 1.47 4.11 11.87 2002 4227 No. 3949 3623 1649 8097 874 4563 11,785 31,807#/hour 0.19 0.17 0.08 0.38 0.04 0.22 0.56 1.65#/acre 0.77 0.71 0.32 1.59 0.17 0.90 2.31 6.84 2003 4751 No. 6057 8489 6838 8527 3193 5739 6740 45,895#/hour 0.25 0.34 0.27 0.35 0.13 0.23 0.27 1.86#/acre 1.19 1.67 1.34 1.67 0.63 1.13 1.32 9.02 2004 5674 No. 7175 6748 4553 8989 3096 6386 10,016 47,229#/hour 0.23 0.22 0.15 0.29 0.10 0.21 0.33 1.55#/acre 1.41 1.33 0.89 1.77 0.61 1.25 1.97 9.28 13 I Table 7. Selected fish species harvested by anglers at Wolf Creek Lake.# Chan. White Wiper Smallmouth All Anglers catfish bass hybrid Bass LM Bass Crappie Walleye fish>12" >24" <13" >18" >21" >14" >18" 1999 9008 No. 1628 1149 7 356 116 14 725 1669 6007#/hour 0.03 0.02 <0.01 0.01 <0.01 <0.01 0.01 0.03 0.13#/acre 0.32 0.23 <0.01 0.07 0.02 <0.01 0.14 0.33 1.15 2000 6865 No. 2258 859 3 198 20 10 316 533 4366#/hour 0.07 0.02 <0.01 0.01 <0.01 <0.01 0.01 0.01 1.13#Iacre 0.44 0.17 <0.01 0.04 <0.01 <0.01 0.06 0.10 1.35<13" >16" <18" >18" 2001 7449 No. 2779 1046 12 126 69 4 415 1609 36 6291#Ihour 0.08 0.03 <0.01 0.01 <0.01 <0.01 0.01 0.05 <0.01 0.18#/acre 0.55 0.21 <0.01 0.02 0.01 <0.01 0.08 0.32 0.01 1.23 2002 4227 No. 1161 378 7 85 62 7 184 862 326 3841#/hour 0.08 0.02 <0.01 <0.01 <0.01 <0.01 0.01 0.04 0.01 0.18#Iacre 0.23 0.07 <0.01 0.02 0.01 <0.01 0.04 0.17 0.06 0.83<16" >20" <18" >26" 2003 4751 No. 2457 1233 16 364 24 1 234 1244 26 5638#/hour 0.10 0.05 <0.01 0.01 <0.01 <0.01 0.01 0.05 <0.01 0.49#/acre 0.48 0.24 <0.01 0.07 <0.01 <0.01 0.05 0.24 <0.01 0.93 2004 5674 No. 2989 1494 18 371 0 3 386 2327 7 7662#/hour 0.10 0.05 <0.01 0.01 0 <0.01 0.01 0.08 <0.01 0.25#/acre 0.59 0.29 <0.01 0.07 0 <0.01 0.07 0.46 <0.01 1.51 14 N NI Main Lake Area 6 Figure 1. Fishery sampling location on Wolf Creek Lake.15 y WOLF CREEK GENERATING STATION Wolf Creek Lake 2005 FISHERY MONITORING REPORT AND 2006 PLAN Prepared by: Supervisor Regulatory Support Approval: Manager Regulatory Affairs Approval:___ 2/16/06 Dan Haines Date____ ____ ____ ____ ____ ___02/17/06 Robert Hammond Date KevinMole02/21/06 Kevin Moles Date EXECUTIVE

SUMMARY

Monitoring during 2005 demonstrated that the fishery in Wolf Creek Lake remained in good condition with no adverse trends identified.

Fish predation pressure on the gizzard shad population continued to prevent excessive shad impingement problems at the circulating water intake. Fishery monitoring activities in 2006 as outlined in this report will continue to measure long-term trends and help Wolf Creek Generating Station prepare for any short term changes, particularly for any changes in the potential for shad impingement events.Public angling on the lake did not impact the fishery's function of supporting plant operations.

The catch and release philosophy promoted when the lake was opened for the public has been compatible with gizzard shad control objectives.

Monitoring data from 2004 warranted management activities to improve the fishery for public use. The following were recommended to the Kansas Department of Wildlife Parks (KDWP): 1. Increase of the creel limit for crappie greater than 14 inches from two to ten fish per day to increase angler use and increase harvest of older crappie.2. Increase the catfish creel limit from five to ten fish per day to be consistent with statewide creel limits. Catfish are not considered a significant predator of gizzard shad.3. Decrease the wiper length limit from 24 to 21 inches to increase harvest of older fish.The KDWP accepted and changed the following beginning for 2006: 1. Crappie creel limits were not changed due to perceptions of angler dissatisfaction.

2. Increased catfish creel limit to ten per day.3. Decreased wiper length limit from 24 to 21 inches.Based on 2005 monitoring, the following are recommended:

1. Maintain current 2006 creel and/or length regulations through 2007.2. Investigate walleye age structure, total annual mortality, and mortality caps to determine if current size and creel regulations are appropriate.

3. Stock a 2006 wiper year-class within budget constraints, and budget for a 2007 stocking at a rate of 10 two-inch fish per acre (50,000).2 2005 FISHERY MONITORING REPORT AND 2006 PLAN INTRODUCTION This report presents the results of fishery monitoring activities on Wolf Creek Lake (WCL). Data are summarized in table form to document long-term trends and demonstrates that the fishery has functioned as desired through 2005. The goal is to increase public safety and plant operating efficiency by reducing the potential for excessive gizzard shad young-of-year (YOY) impingement on the Circulating Water System intake screens. Shad impingement problems to date have not occurred due largely to the characteristics of the current fishery.Public use of the fishery is also important to maintain community relations and local economic benefits.

Consequently, maintaining and/or enhancing public enjoyment of the fishery that is compatible with the shad impingement control are other important goals of this program. Creel and length limits were determined jointly with the Kansas Department of Wildlife and Parks (KDWP).The catch-and-release strategy employed appears to have succeeded with no detrimental changes to the fishery observed through 2005.Fishery monitoring activities in 2006 will be similar to 2005 to maintain long-term trending.

Short-term changes will also be detected to ensure WCGS can be prepared if impingement potential increases.

METHODS The monitoring methods used during 2005 allowed for continued analyses of important long-term trends. Gill netting was used at long-term sites on WCL (Figure 1). Spring electrofishing effort targeted smallmouth and largemouth bass habitat by shocking in shoreline transects until a minimum number of fish or a designated length of shore was sampled. Small-mesh gill netting replaced shoreline seining in 1998 to better assess young-of-year (YOY) gizzard shad densities and recruitment (Boxrucker et al -1991). Important species to the fishery were targeted when expected to be efficiently sampled.Sampling efforts are listed in Table 1. Fish sampled were weighed to the nearest gram, and measured (total length, TL) to the nearest millimeter.

Proportional stock density (PSD, Anderson 1980), incremental relative stock density (RSD, Gablehouse 1984), and relative weight (Wr, Wege and Anderson 1978) were indices applied. Length-weight equations adopted by KDWP were used.The 2006 efforts will be completed as scheduled in Table 2. These efforts are the same as for 2005. Anglers using the lake park report the number of fish caught and released, the number kept for personal use, and angler satisfaction.

These creel sheets are collected and tabulated by Coffey County. Data from the census sheets will be used to determine if harvest rates change dramatically and to measure angler success.Increasing walleye size variability and maximum size is advantageous to diversified shad control, as well as angler compatibility and success. Consequently, walleye age structure, total annual mortality, and mortality caps will be determined using methods similar to Quist et. al. (2004). The current management objective is to produce larger walleye (>26 inches total length) by encouraging harvest of smaller walleye from a stable population with good recruitment, thus reducing intraspecific competition allowing surviving individuals to grow larger. A slot limit prohibiting harvest of fish between 18 and 26 inches was set to accomplish this. Assessing mortality caps will determine if walleye die of natural mortality before reaching 26 inches, if harvest of smaller individuals is necessary, if decreasing interspecific competition for available prey would be effective, and if regulating length of harvest is applicable given current lake biology and angler 3 impacts. University graduate students will be solicited and supported with research grant funding to complete this task. Available scale and fishery data will be used.RESULTS AND PLANS The fishery in Wolf Creek Lake continued to function as desired. It exhibited signs of low prey densities, which is preferred to minimize fish impingement at the circulating water intake. The potential for excessive gizzard shad impingement remained small due to relatively low YOY densities going into the winter months. The shad appear to be limited by predation, as indicated by the population indices of the predator species. Gizzard shad typically has been an important forage species in most reservoirs (Carlander 1969, Pflieger 1975, Stein and Johnson 1987, Colvin 1993). For shad to be compatible with WCGS operation, low YOY shad densities must be maintained.

Periodic recruitment of shad young to reproducing adults also must occur to maintain the predators, which in turn control shad numbers. These conditions currently exist in WCL, and benefit WCGS.Catch densities of remained similar to past years for adult gizzard shad, white bass and wiper;increased for white crappie, and decreased for smallmouth bass and walleye (Table 3). Fall densities of small gizzard shad remained low. Density changes for smallmouth bass is likely due to sampling variation.

Walleye changes may be due to sampling variation because catch densities were within past ranges. Increased angler harvest for two consecutive years may also have contributed (Table 7)Fish length frequencies in 2005, as shown by the PSD/RSD indices (Table 4), showed no major changes to past years, except for gizzard shad. A higher PSD indicates fewer shad recruiting to mid-size due in part to predation, and an older population existing.

Continued recruitment and growth of important species were evident with most showing good percentages of mid-sized individuals (RSDS-Q, RSD Q-P, and RSD M-T size ranges). For wipers, the sizes increased slightly showing continuing maturation of the latest 2001 year-class stocking.

Because of this, budgeting for potential wiper stocking in 2006 is recommended to ensure continued presence.There was a small shift to larger walleye, possibly due to the current regulations, but this shift is not definitive.

Walleye research referenced earlier should determine any relationships.

Body conditions as indicated by Wr indices (Table 5) remained similar to past years for gizzard shad, smallmouth bass, and white crappie; increased for white bass, wiper; and decreased for walleye. All species showed adequate body conditions to maintain their populations.

Large increases or decreases in body condition were not evident for most species. The white bass increase may be attributable to decreasing wiper competition, as the 2001 year-class matures.Overall, this indicates that no large changes in prey availability occurred, primarily gizzard shad densities.

No detrimental impacts due to angler harvest of the predator populations controlling gizzard shad have been observed.

Harvest rates were slightly lower, but still similar for most species, except walleye (Table 6 and 7). Harvest of walleye under 18 inches nearly doubled in 2004, and slightly more in 2005. Because the population indices for catch frequency, length frequency, and body conditions remained similar to past years, influence by angler harvest was not apparent.There are no fish creel and length limit changes recommended for 2007. The current smallmouth bass and walleye slot limits were imposed to increase body condition and growth. These limits should remain in effect until more data is collected to assess their impacts. The current minimum length limit (12 inches) for white bass was set to protect younger wipers. Since a wiper year class stocking is planned for 2006 and 2007, the white bass minimum length should remain in effect.The crappie is an important littoral predator of gizzard shad in the absence of high largemouth densities, so the minimum length limit (14 inches) was set to protect a majority of the larger 4 individuals.

A large proportion of crappie were near the limit (PSD M-T of 28, Table 4), consequently the limit should remain the same.PLAN RESULTS To ensure continued WCGS support and public use, the fishery program will accomplish the following:

1. Continue monitoring as outlined.2. Maintain current 2006 creel and/or length regulations through 2007.3. Investigate walleye age structure, total annual mortality, and mortality caps to determine if current size and creel regulations are appropriate.

4. Stock a 2006 wiper year-class within budget constraints, and budget for a 2007 stocking at a rate of 10 two-inch fish per acre (50,000).Thank you very much.5 LITERATURE CITED Anderson, R. 0. 1980. Proportional stock density (PSD) and relative weight (Wr): interpretive indices for fish populations and communities.

Pages 27-33 in S. Gloss and B. Shupp, editors. Practical fisheries management:

More with less in the 1980's. New York Chap., Amer. Fish. Soc., Workshop Proceedings.

Boxrucker, J., D. Degan,.D.

DeVries, P. Michaletz, M. J. Van Den Avyle, B. Vondracek.

-1991 (year not specified).

Sampling Shad in Southern Impoundments.

U.S. Fish and Wildlife Service, Reservoir committee of the Southern Division-American Fisheries Society, Coop agreement No. 14-16-0002-91-216.

22 pp.Carlander, K. D. 1969. Handbook of Freshwater Fisheries Biology, Vol. 1. Iowa State University Press, Ames, Iowa. 752 pp.Colvin, Mike. 1993. Ecology and management of white bass: a literature review. Missouri Department of Conservation, Dingell-Johnson Project F-I-R-42, Study 1-31, Job 1, Final Report.Gablehouse, D. W., Jr. 1984. A length-categorization system to assess fish stocks. North American Journal of Fisheries Management.

Vol. 4. P 273-285.Pflieger, W. L. 1975. The Fishes of Missouri.

Missouri Department of Conservation.

343 pp.Quist, M. C., J. L. Stephen, C. S. Guy, and R. D. Schultz. 2004. Age Structure and Mortality of Walleyes in Kansas Reservoirs:

Use of Mortality Caps to Establish Realistic Management Objectives.

North American Journal of Fisheries Management, 24:990-1002.

Stein, R. A. and B. M. Johnson. 1987. Predicting carrying capacities and yields of top predators in Ohio impoundments.

Ohio Department of Natural Resources, Division of Wildlife.

Federal Aid in Fish Restoration Project F-57-R-5 through R-9, Study 12. 144 pp.Wege, G. J. And R. 0. Anderson.

1978. Relative weight (Wr): a new index of condition for largemouth bass. Pages 79-91 in G. D. Novinger and J. G. Dillard, editors. New approaches to the management of small impoundments.

North Central Division, American Fisheries Society. Special Publication 5, Bethesda, MD.6 Table 1. Fishery sampling effort by gear type used at Wolf Creek Lake during 2005.Water Gear Date (1) Location Effort Temp °F Electrofishing

'z' 5/27 NA (3)0.75 72 Standard Gill Netting (4) 10/11 2 (5) 1 66-69 9 1 77-86 10/12 6 1 65-67 8 1 64-65 10/13 2 1 68 9 1 70-85 10/14 6 1 67 8 1 64 Small Mesh Gill Netting (6) 10/26 6 (7)2 59-62 8 2 59 10/27 6 2 60 8 2 57 Fyke Netting 10/26 2 (8) 1 56 6 1 62 8 1 59 10/27 2 1 60 6 1 60 8 1 57 (1)(2)(3)(4)(5)(6)(7)(8)See Figure 1 for locations.

Equipment consisted of a boat-mounted Smith-Root unit operated at 220v, 9-10 amp, DC current pulsed 120 cycles/second Shock effort shown as hours water was energized.

Standard gill nets consisted of a complement of four 8'x100' monofilament nets, one each of 1", 1.5", 2.5", and 4" uniform mesh.Standard gill netting effort listed as number of net-complement-nights set.Small-mesh gill nets consisted of a complement of two 8'x100' monofilament nets, one with 0.5", and the second with 0.75" uniform mesh.Small-mesh gill netting effort listed as number of small-mesh-complement-nights set.Fyke netting effort listed as number of trap-net-nights.

7 Table 2. Fish Sampling Schedule at Wolf Creek Lake during 2006.Minimum Information Needed to Assess Fishery Method Preferred Time Frame 1.2.3.4.5.6.7.8.Gizzard shad recruitment through winter White crappie population characteristics and health Largemouth bass population characteristics and health Smallmouth bass population characteristics and health White bass population characteristics and health Wiper survival and health Walleye population characteristics and health Gizzard shad YOY reproduction and densities going into winter Electrofishing Fyke netting/Gill netting Electrofishing Electrofishing Gill netting Gill netting Gill netting Small Mesh Gill Netting April/May October/November April/May April/May October October October September/October 8

Table 3. Catch-per-unit-of-effort (CPUE) of selected fish species in Wolf Creek Lake. Fall gill net, Fyke net, and electrofishing data were not collected in 2001 due to the September 11 events.Gizzard Gizzard Smallmouth Largemouth White Shad Shad (YOY) White bass Wiper Bass Bass Crappie Walleye 1983 (1)7 (1) 23 (1) 15 (2) 24.5 (3) 0 (1) 4 1984 25 18 11 45.0 6 29 1985 3 6 22 45.3 5 26 1986 32 25 14 (2) 1.3 34.5 5 9 1987 10 18 21 8.5 18.8 12 16 1988 12 28 26 10.5 22.0 9 19 1989 18 17 23 14.8 32.3 4 22 1990 10 34 12 12.0 14.0 5 13 1991 14 45 22 20.5 5.5 4 19 1992 19 17 9 10.8 8.3 6 22 1993 11 52 8 15.0 5.0 5 12 1994 9 61 11 12.5 2.0 4 23 1995 25 29 11 6.3 2.0 5 16 1996 9 (4)22.9 19 3 10.8 0.3 9 20 1997 19 77.0 60 8 5.5 1.3 4 28 1998 18 39.9 45 6 10.5 1.5 3 16 1999 15 9.9 37 4 11 3.3 6 14 2000 18 29.4 36 13 21.5 3.0 (5)9 28 2001 -----2.0 -2002 11 3.5 32 4 2.0 1.0 6 8 2003 10 1.9 54 9 8.0 2.0 7 14 2004 12 5.5 33 6 34 0.8 -20 2005 11 0.3 37 4 16 0.0 13 9 (1) Data from fall standard gill netting. Units equal number per gill-net-complement-night

> stock size.(2) Data from spring electrofishing.

Units equal number per hour shocked > stock size. Shocking efforts starting in 2004 targeted prime habitats rather than standard locations as completed during prior years.(3) Data from spring Fyke netting. Units equal number per trap-net-night

> stock size.(4) Data from smallmesh gill net. Units equal number per net complement of one 0.5 and one 0.75 mesh net.(5) Data beginning in 2000 were from fall Fyke netting. Netting not completed during 2004 due to adverse weather. Units equal number per trap-net-night

> stock size.9 Table 4. Proportional Stock Density (PSD) and Relative Stock Density (RSD) for selected fish species at Wolf Creek Lake.Stock (S), quality (Q), preferred (P), memorable (M), and trophy (T) size ranges are per Gablehouse (1984). Fall gill net, Fyke net, and electrofishing data were not collected in 2001 due to the September 11 events.Species Index 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 05 Gizzard PSD 85 90 10 70 81 93 59 69 84 75 94 81 30 -87 49 47 83 0 15 10 0 30 19 shad (1)(2)White bass (1)(2)Wiper(1)RSD-P 7 41 31 16 25 6 19 70 PSD RSD S-Q RSD Q-P RSD P-M RSD M-T RSD T+PSD RSD S-Q RSD Q-P RSD P-M RSD M-T RSD T+Smallmouth PSD Bass(4), (5 after RSD S-Q 2003)RSD Q-P RSD P-M RSD M-T RSD T+Largemouth PSD 77 85 27 59 80 31 89 63 56 57 59 45 65 23 15 73 41 20 69 11 37 44 43 41 55 35 9 7 2 10 36 5 12 8 51 4 11 3 4 39 62 21 34 35 24 55 45 0 53 45 40 55 29 15 4 15 9 2 22 11 4 < 1 2 2 7<1 1 10 97 96 10 10 10 10 85 30 88 89 10 10 0 0 0 0 0 0 0 3 4 15 70 12 11 1 10 14 3 32 11 42 40 28 47 39 21 6 4 33 73 91 58 58 50 53 53 61 76 92 81 30 23 5 9 42 1 1 2 29 37 40 61 40 44 40 52 58 50 52 77 70 71 63 60 39 60 56 60 48 42 50 48 23 30 8 25 10 22 26 17 20 28 28 23 29 34 28 17 10 27 32 13 20 12 20 26 18 21 36 40 4 5 4 6 1 7 8 4 5 9 2 7 2 1 92 99 97 10 82 85 88 10 10 60 50 10 10 0 0 0 0 0 8 1 3 18 15 12 40 50 50 19 28 19 5 12 10 13 13 20 17 50 72 71 80 95 71 71 75 88 10 40 33 10 0 0-13 51 53 17-48 33 53 41-52 67 47 59-10 1 5 3-34 29 43 32 4 3 5 5<1 10 10 10 10 0 0 0 0-24 3-31 20 65 55-45 80 33 39 2-88 83 66 50-13 17 34 50-38 17 22 17-50 63 36 25 4 8 8 Bass (5)RSD S-Q RSD Q-P RSD P-M RSD M-T RSD T+88 50 10 0 13 25 38 25 17 50 50 83 (7) (7)10 11 Table 4. (cont.)Species Index 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 05 White PSD 99 10 10 10 10 95 10 10 99 10 10 10 82 -98 99 97 87 0 0 0 0 0 0 0 0 0 crappie (6)(8) RSD S-Q 1 5 1 18 -2 1 3 13 RSD Q-P 2 12 9 3 3 2 8 1 9 9 9 43 -34 48 32 53 RSD P-M 4 10 13 7 26 14 44 11 12 15 12 13 11 -11 29 15 6 RSD M-T 85 60 70 87 63 75 41 87 72 71 74 77 28 -52 21 47 28 RSD T+ 10 21 10 3 8 4 7 1 6 5 5 1 1 -1 1 3 Walleye 1) PSD 94 93 96 77 93 90 52 83 73 31 55 74 78 -47 60 69 62 RSD S-Q 6 7 4 23 7 10 48 17 27 69 45 26 22 -53 40 31 38 RSD Q-P 81 80 95 59 74 67 41 82 67 28 51 74 75 -40 57 66 54 RSD P-M 14 13 1 18 19 22 10 1 6 3 4 3 -8 3 3 7 RSD M-T RSD T+(1) Data from fall gill netting.(2) Corrected for gill net efficiency (Willis et al 1985)(3) Data from spring electrofishing.

(4) Data from fall electrofishing.

(5) Data from spring Fyke netting.(6) Data from spring Fyke netting 1999 and earlier, from fall Fyke netting 2000 and later.(7) Insufficient data to calculate.

(8) 2004 data from fall gill netting.12 Table 5. Relative weight (Wr) of selected fish species in Wolf Creek Lake. Wr formulas from KDWP were used. Per Wege and Anderson (1978), Wr values of 100 and higher represent fish at or above the 75 percentile, values of 93 to 100 are between the 50 and 75 percentile, values of 86 to 93 are between the 25 and 50 percentile, and values less than 86 are below the 25 percentile.

Fall gill net, Fyke net, and electrofishing data were not collected in 2001 due to the September 11 events.Gizzard Smallmouth Largemouth White Shad White bass Wiper Bluegill Bass Bass Crappie Walleye 1983 (1) 85 (1) 78 (1) 90 (2) 107 (2) 97 (4) 107 (1) 78 1984 87 94 86 103 98 93 82 1985 88 89 78 102 97 94 83 1986 85 86 84 111 93 93 81 1987 89 93 89 105 (3) 97 88 89 80 1988 90 94 85 108 92 92 102 81 1989 104 95 80 96 92 87 88 88 1990 100 99 82 121 104 84 98 85 1991 93 93 78 111 91 79 99 86 1992 93 92 88 102 91 84 95 86 1993 93 94 88 92 91 80 85 85 1994 93 90 75 104 86 75 97 85 1995 88 97 88 124 90 89 105 85 1996 89 106 100 121 100 57 104 94 1997 89 97 89 105 81 90 99 88 1998 81 90 83 83 86 91 95 76 1999 82 93 83 105 90 78 97 81 2000 76 86 77 106 85 78 (5)88 80 2001 --102 -84 2002 87 88 75 110 82 89 (5)95 77 2003 85 88 68 116 88 83 96 86 2004 81 87 72 107 84 (5) (1)91 86 2005 83 95 80 (5) 84 (5) 89 81 (1) Data from fall gill netting.(2) Data from spring electrofishing.

(3) Data from spring Fyke netting.(4) Data from fall Fyke netting.(5) Insufficient sample size to calculate.

13 Table 6.Selected fish species cauqht and released by anllers at Wolf Creek Lake.Chan. White Wiper Smallmouth All Anglers catfish bass ]hybrid I Bass LM Bass Crappie Walleye fish 1999 9008 No. 6928 15,171 3503 17,482 3885 7382 31,027 86,464#/hour 0.15 0.32 0.07 0.37 0.08 0.15 0.65 1.82#/acre 1.36 2.98 0.69 3.43 0.76 1.45 6.10 16.99 2000 6865 No. 5191 7838 2267 12,579 4918 5536 21,599 61,102#Ihour 0.15 0.23 0.07 0.36 0.14 0.16 0.63 1.77#/acre 1.02 1.54 0.45 2.47 0.97 1.09 4.24 12.00 2001 7449 No. 5623 8777 1810 10,136 4736 7457 20,911 60,417#/hour 0.16 0.25 0.05 0.28 0.13 0.21 0.59 1.70#/acre 1.10 1.72 0.35 1.99 0.93 1.47 4.11 11.87 2002 4227 No. 3949 3623 1649 8097 874 4563 11,785 31,807#/hour 0.19 0.17 0.08 0.38 0.04 0.22 0.56 1.65#/acre 0.77 0.71 0.32 1.59 0.17 0.90 2.31 6.84 2003 4751 No. 6057 8489 6838 8527 3193 5739 6740 45,895#/hour 0.25 0.34 0.27 0.35 0.13 0.23 0.27 1.86#Iacre 1.19 1.67 1.34 1.67 0.63 1.13 1.32 9.02 2004 5674 No. 7175 6748 4553 8989 3096 6386 10,016 47,229#/hour 0.23 0.22 0.15 0.29 0.10 0.21 0.33 1.55#/acre 1.41 1.33 0.89 1.77 0.61 1.25 1.97 9.28 2005 5287 No. 10,619 8048 2683 7785 1420 4370 9457 44,629#/hour 0.37 0.28 0.09 0.27 0.05 0.15 0.33 1.54#/acre 2.09 1.58 0.53 1.53 0.28 0.86 1.86 8.77 14 Table 7. Selected fish sDecies harvested by analers at Wolf Creek Lake.# Chan. White Wiper Smallmouth All Anglers catfish bass hybrid Bass LM Bass Crappie Walleye fish 1999 9008 No. 1628#/hour 0.03#/acre 0.32 No. 2258#/hour 0.07#/acre 0.44 2000 6865 2001 2002 2003 7449 No. 2779#/hour 0.08#/acre 0.55 4227 No. 1161#/hour 0.08#/acre 0.23 4751 No. 2457#/hour 0.10#/acre 0.48 5674 No. 2989#Ihour 0.10#/acre 0.59 5287 No. 2541#/hour 0.09#/acre 0.50>12" 1145 0.02 0.23 859 0.02 0.17 1046 0.03 0.21 378 0.02 0.07 1233 0.05 0.24 1494 0.05 0.29 1281 0.04 0.25>24" 7<0.01<0.01 3<0.01<0.01 12<0.01<0.01 7<0.01<0.01 16<0.01<0.01 18<0.01<0.01 8<0.01<0.01<13" 0.01 0.07 198 0.01 0.04<13" 126 0.01 0.02 85<0.01 0.02<16" 0.01 0.07 371 0.01 0.07 303 0.01 0.06>18" 116<0.01 0.02 20<0.01<0.01>16" 69<0.01 0.01 62<0.01 0.01>20" 24<0.01<0.01 0 0 0 10<1.01<0.01>21" 14<0.01<0.01 10<0.01<0.01 4<0.01<0.01 7<0.01<0.01 1<0.01<0.01 3<0.01<0.01 6<0.01<0.01>14" 725 0.01 0.14 316 0.01 0.06 415 0.01 0.08 184 0.01 0.04 234 0.01 0.05 386 0.01 0.07 325 0.01 0.06>18" 1669 0.03 0.33 533 0.01 0.10<18" >18" 1609 36 0.05 <0.01 0.32 0.01 862 326 0.04 0.01 0.17 0.06<18" >26" 1244 26 0.05 <0.01 0.24 <0.01 2327 7 0.08 <0.01 0.46 <0.01 2441 8 0.08 <0.01 0.48 <0.01 6007 0.13 1.15 4366 1.13 1.35 6291 0.18 1.23 3841 0.18 0.83 5638 0.49 0.93 7662 0.25 1.51 6981 0.24 1.37 2004 2005 15 N N III Main Lake Area 6 Figure 1. Fishery sampling location on Wolf Creek Lake.16 Enclosure 6 to ET 07-0001 Maps Map of John Redmond Reservoir Map of Flint Hills National Wildlife Refuge JOHN REDMOND RESERVOIR PUBLIC HUNTING AREA Ril1E 1111El "% 11 R12E1, R13Eý1I , 1113 E 1 14E , Ii RISE I I 4 19 s IF T 20 S n I I __r 1 .-~A k 0 1 r~~ N~' I~tI*I II 6-I./i II~.3o)~'I 1 II 1t11R4 oA6JA?~~F II '~-~m 'ýT EM ORIA Y-o'T KA 4 36 NE IOSHO RAPIDS 3l oj ~ ~.-cc 0 (3 c.o~(.r -LEBO I'F (f.., , 36 31 (I 36] 3 J/: I I I I * ! J ../ i IIJ II f II I 6~1L~ ~6/Ik~j ~ifl tmo~==4=~I

~ _____ ii- ~ ~i ~ JJ .L.. IL ILtd..~.2I

_____ IL.B~ II II I f I , II Dal-.I I.-'0 Area Riverside East Riverside West 0 Otter Creek 0 Strawn Ramp 0 Hartford Ramp.:-I(F..-1-H RTFO~ D 2-IL ~i'it-71t~.a-.. .. I i # I r J II .. ... .I--h-EEN Tl 11U1ET i 20pl 36_- 31~~l I S ~-vi Tl I re 241 Paved Road Improved Road--- Project Boundary-Corps Areas Open For Hunting -No Center Fire Weapons Non-Toxic Shot Only State Areas Open For Hunting -Non-toic Shot Only Refuge Area Open SHunting -No Center Fie Weapons Non-Toxc Shot Only 0 1 2 Scale of Miles HILLS REFU E NE ST-JL J6 11 ---0 C C Pro. 0 0 0 IL-011PLINGTON-J 0 01 W RAWN Army Corps of Engineers Tulsa District RESERVOIR DATA Top .1 conservation pool El. 1039.0 52 shoreline miles at El. 1039.0 Total project land & water acreage 29798 US 2000 Flint Hiifs 0 0 C 0 National Wgidlife Refuge Darbyshi r Lake 22nd Rd f-i 0 I,.CR 110:.-i 11 i_Rituge IHuadqui m us-1Boat RamD'11-n w C 0.L a H.19th Rd Legend I Refuge Boundary-Gravel Roads-Paved Roads Water EaI Marshes Open to permitted activities year-round I- Open to permitted acti viti es except waterfovl hunting.Area closed from Nov. 1 to March 1 to all public entry Open to non-hunting permitted acti vites except during Nov. 1 to March 1 when area is closed to all public entry Open to non-hunting activities year-round To Hwy I Lr Ln 16th Rc 4 N c: SCALE IN MILES Enclosure 7 to ET 06-0001 Tables of Wildlife Present Within the Vicinity or Transmission Line Corridor for Wolf Creek Table 1, Representative amphibian and reptile species that may occur in the vicinity of WCGS or within the applicable transmission line corridors Table 2, Representative bird species that may occur in the vicinity of WCGS or within the associated transmission line corridors Table 3, Representative mammal species that may occur in the vicinity of WCGS or within the associated transmission line corridors Cited Literature Table 1. Representative amphibian and reptile species that may occur in the vicinity of WCGS or within the applicable transmission line corridors.

Mixed Wetland/ Wood- woodland/Species aquatic land prairie Prairie Amphibians Smallmouth Ambystoma x salamander texanum Tiger salamander Ambystoma x tigrinum American toad Bufo americanus x Great plains toad Bufo cognatus x Woodhouse's toad Bufo woodhousii x Northern cricket frog Acris crepitans x Cope's gray treefrog Hyla versicolor x Spotted chorus frog Pseudacris clarkii x Boreal chorus frog Pseudacris maculate Western chorus frog Pseudacris x triseriata Crawfish frog Rana areolata x Plains leopard frog Rana blairi x Bullfrog Rana catesbeiana x Southern leopard frog Rana x sphenocephala Great plains Gatrophryne x narrowmouth toad ofivacea Reptiles Snapping turtle Chelydra x serpentina Common musk turtle Sternotherus x odoratus Painted turtle Chrysemys picta x Mississippi Map turtle Graptemys kohnii x False map turtle Graptemys x pseudogeographica River cooter Pseudemys x concinna Eastern box turtle Terrepene carolina x Ornate box turtle Terrepene ornata x Slider Trachemys scripta x Spiny softshell Apalone spinifera x Collared lizard Crotaphytus collaris x x Texas horned lizard Phrynosoma x cornutum Five-lined skink Eumeces fasciatus x Great plains skink Eumeces obsoletus x

Table 1. (Continued)

Mixed Wetland/ Wood- woodland/Species aquatic land [_prairie Prairie Southern prairie skink Eumeces x x obtusirostris Ground skink Scincella lateralis x Six-lined racerunner Cnemidophorus x sexlineatus Western slender glass Ophisaurus x x lizard attenuatus Ringneck snake Diadophis x punctatus Eastern hognose heterodon x snake platirhinos Flathead snake Tantilla gracilis x Plains blackhead Tantilla nigriceps x snake Racer Coluber constrictor x Great plains rat snake Elaphe emoryi x Rat snake Elaphe obsoleta x Prairie kingsnake Lampropeltis x x calligaster Common kingsnake Lampropeltis getula x Milk snake Lampropeltis x trangulum Rough green snake Opheodrys x aestivius Gopher snake Pituophis catenifer x x x Ground snake Sonora x semiannulata Plainbelly water snake Nerodia x erythrogaster Diamond back water Nerodia rhombifer x snake Northern Water snake Nerodia sipedon x Graham's crayfish Regina grahamii x snake Western ribbon snake Thamnophis x proximus Plains garter snake Thamnophis radix x Common garter snake Thamnophis sirtalis x x x Lined snake Tropidoclonion x x lineatum Copperhead Agkistrodon x contortrix Massasauga Sistrurus catenatus x x x Table 2. Representative bird species that may occur in the vicinity of WCGS or within the associated transmission line corridors.

Mixed Urban Wetland/ Prairie/ Prairie/ and Species Aquatic Woodland Woodland open area Other Ducks/Geese/Swans Greater white-fronted goose x Snow goose x Canada goose x Trumpeter swan x Tundra swan x Wood duck x x Gadwall x American wigeon x American Black Duck x Mallard x Blue-winged teal x Cinnamon teal x Northern Shoveler x Northern pintail x Green-winged teal x Canvasback x Redhead x Ring-necked duck x Lesser Scaup x Bufflehead x Common goldeneye x Hooded merganser x x Common merganser x Red-breasted merganser x Ruddy duck x Pheasants/GrouselQuail Ring-necked pheasant x x Greater prairie chicken x Wild turkey x x Northern bobwhite x x Misc waterbirds Common loon x Pied-billed grebe x Horned grebe x Eared grebe x American white pelican x Double-crested cormorant x American bittern x Least bittern x Great blue heron x Table 2. (Continued)

Mixed Urban Wetlandl Prairie/ Prairie/ and Species Aquatic Woodland Woodland open area Other Great egret x Little blue heron x Cattle egret x Green heron x Black-crowned night heron x Yellow-crowned night heron x White-faced ibis x Sandhill crane x Hawks/Falcons/EaglesNul tures Osprey x Northern harrier x x Sharp-shinned hawk x x Cooper's hawk x Northern goshawk x Red-shouldered hawk x Broad-winged hawk x x Swainson's hawk x x Red-tailed hawk x x Rough-legged hawk x x American kestrel x x Merlin x Peregrine falcon x x Prairie falcon x Bald eagle x x x Turkey vulture x x Rails/Gallinules King rail x Sora x American coot x Plovers/Sandpipers/Shore birds Black-bellied plover x American golden plover x Semipalmated plover x Killdeer x x American avocet x Black-necked stilt x Greater yellowlegs x Lesser yellowlegs x Solitary sandpiper x Willet x Table 2. (Continued)

Mixed Urban Wetland/ Prairie/ Prairie/ and Species Aquatic Woodland Woodland en area Other Spotted sandpiper x Upland sandpiper x Hudsonian godwit x Ruddy turnstone x Sanderling x Semipalmated sandpiper x Western sandpiper x Least sandpiper x Whiter-rumped sandpiper x Baird's sandpiper x Pectoral sandpiper x Dunlin x Short-billed dowitcher x Long-billed dowitcher x Common snipe x American woodcock X Wilson's phalarope x Gulls/Terns Franklin's gull x x Ring-billed gull x x Glaucous gull x Herring gull x Caspian tern x Forster's tern x Least tern x Black tern x Pigeons/Doves Rock dove x Mourning dove x x Cuckoos Black-billed cuckoo x Yellow-billed cuckoo x Owls/Goatsuckers Barn owl x Great-horned owl x x Snowy owl x Barred owl x Long-eared owl x x Short-eared owl x Eastern screech owl x X Common nighthawk x

Table 2. (Continued)

Mixed Urban Wetland/ Prairie/ Prairie/ and Species Aquatic Woodland Woodland open area Other SwiftslHummingbirds Chimney swift x Ruby-throated hummingbird x x x Kingfishers Belted kingfisher x Woodpeckers Red-headed woodpecker x x Red-bellied woodpecker x x Downy woodpecker x x Hairy woodpecker x x Northern flicker x x Pileated woodpecker x Flycatchers Olive-sided flycatcher x Eastern wood-pewee x Willow flycatcher x x Least flycatcher x x Eastern phoebe x Great-crested flycatcher x Western kingbird x x Eastern kingbird x x Scissor-tailed flycatcher x Shrikes Northern shrike x Loggerhead shrike x x Vireos Bell's vireo x Solitary vireo x Yellow-throated vireo x Warbling vireo x x Red-eyed vireo x Jays/Crows Blue jay x x American crow x x Larks Horned lark x Table 2. (Continued)

Mixed Urban Wetland/ Prairie/ Prairie/ and Species Aquatic Woodland Woodland open area Other Swallows Purple martin x Tree swallow x Northern rough-winged x swallow Bank swallow x x Cliff swallow x Barn swallow x x Chickadees/Titmice Black-capped chickadee x Carolina chickadee x Tufted titmouse x Nuthatches/Creepers White-breasted nuthatch x x Brown creeper x Wrens Carolina wren x x House wren x x Winter wren x Kinglets Golden-crowned kinglet x Ruby-crowned kinglet x Gnatcatchers Blue-gray gnatcatcher x Thrushes Eastern bluebird x x Veery x Wood thrush x American robin x x Thrashers Gray catbird x Northern mockingbird x Brown thrasher x Starlings European starling x_ _ _ _ _ _ _ _ I I_ I1 I_ _ _ _

Table 2. (Continued)

Mixed Urban Wetland/ Prairie/ Prairie/ and Species Aquatic Woodland Woodland open area Other Pipits Water pipit x Waxwings Cedar waxwing x Warblers Nashville warbler x Northern parula x Yellow warbler x Chestnut-sided warbler x Magnolia warbler x Yellow-rumped warbler x Blackburnian warbler x Bay-breasted warbler x Cerulean warbler x Black-and-white warbler x American redstart x Ovenbird x Northern waterthrush x x Kentucky warbler x Common yellowthroat x Wilson's warbler x x Tanagers Summer tanager x Sparrows Rufous-sided towhee (???) x American tree sparrow x Chipping sparrow x x Field sparrow x Vesper sparrow x Lark sparrow x x Savannah sparrow x Grasshopper sparrow x Fox sparrow x x Song sparrow x x Lincoln's sparrow x Swamp sparrow x White-throated sparrow x White-crowned sparrow x Harris' sparrow x x Dark-eyed junco x x Lapland longspur x Table 2. (Continued)

Mixed Urban Wetland/ Prairie/ Prairie/ and Species Aquatic Woodland Woodland en area Other Snow bunting x x Grosbeaks/Buntings Northern cardinal x x X Rose-breasted grosbeak x Blue grosbeak X Indigo bunting x Dickcissel X Blackbirds/Orioles Bobolink x Red-winged blackbird x Eastern meadowlark x Western meadowlark x Yellow-headed blackbird x Rusty blackbird x Brewer' blackbird x Great-tailed grackle x Common grackle x x Brown-headed blackbird x Orchard oriole x x Northern oriole x x Northern finches Purple finch x Pine siskin x x American goldfinch x x House finch x x Old world sparrow House sparrow x______________________________

I ___________

I I I ___________

I___________

Table 3. Representative mammal species that may occur in the vicinity of WCGS or within the associated transmission line corridors.

Mixed Wet- Prairie/ Prairie/ Urban land/ Wood- Wood- open and Species Aquatic land land area Other Virginia opossum Dide/phis x x virginiana Southern short-tailed Blarina x x x shrew carolinensis Least shrew Cryptotis parva x x x Eastern mole Scalopus x x x aquaticus Eastern pipistrelle Pipistrellus x x X subflavus Big brown bat Eptesicus fuscus x x Red bat Lasiurus borealis x Hoary bat Lasiurus cinerus x x x Nine-banded armadillo Dasypus x x novemcinctus Eastern cottontail Sylvilagus x x x floridanus Black-tailed jackrabbit Lepus californicus x Woodchuck Marmota monax x x Thirteen-lined ground Spermophilus x squirrel tridecemlineatus Franklin's ground Spermophilus x squirrel franklinii Gray squirrel Sciurus x carolinensis Fox squirrel Sciurus niger x x Pocket gopher Geomys bursarius x Hispid pocket mouse Chaetodipus x hispidus Beaver Castor canadensis x Plains harvest mouse Reithrodontomys x montanus _Western harvest Reithrodontomys x x mouse megalotis Deer mouse Peromyscus x maniculatus White-footed mouse Peromyscus x x leucopus Hispid cotton rat Sigmadon hispidus x x Eastern wood rat Neotoma floridana x x Prairie vole Microtus x ochrogaster Woodland vole Microtus pinetorum x x Muskrat Ondatra zibethicus x

Table 3. (Continued)

Mixed Wet- Prairie/ Prairie/ Urban land/ Wood- Wood- open and Species Aquatic land land area Other House mouse Mus musculus x x x x Meadow jumping Zapus hudsonius x mouse Coyote Canis latrans x x Red fox Vulpes vulpes x x x Gray fox Urocyon x cinereoargenteus Racoon Procyon lotor x x Long-tailed weasel Mustela frenata x x x Mink Mustela vison x Badger Taxidea taxus x Striped skunk Mehpitis mephitis x Eastern spotted skunk Spilogale putorius x x Bobcat Lynx rufus x White-tailed deer Odocoileus x x virginianus Literature Cited Timm, R. M., N. A. Slade, G. R. Pisani, J. R. Choate, G. A. Kaufman, and D. W.Kaufman. 2006. Mammals of Kansas. website http://www.ksr.ku.edu

.accessed December 20, 2006.Bee, J. W., G. Glass, R. S. Hoffman, and R. R. Patterson.

1981. Mammals in Kansas.University of Kansas Museum of Natural History, Public Education Seriers No. 7.University of Kansas, Lawrence.

300 pp.Thompson, M. C., and C. Ely. 1989. Birds in Kansas, Volume I. Universtiy of Kansas Museum of Natural History, Public Education Series No. 11. Universtiy of Kansas, Lawrence.

404 pp.Thompson, M. C., and C. Ely. 1992. Birds in Kansas, Volume I1. University of Kansas Museum of Natural History, Public Education Series No. 12. University of Kansas, Lawrence.

424 pp.Collins, J. T. 1993. Amphibians and Reptiles in Kansas. University of Kansas Museum of Natural History, Public Education Series No. 13. University of Kansas, Lawrence.397 pp.Salsbury, G. A. and S. C. White. 2000. Insects in Kansas, Third Edition. Kansas Department of Agriculture and Kansas State University.

Topeka, Kansas. 522pp.Kansas Gas and Electric Company. 1984. Wolf Creek Generating Station 1983-1984 Pre-operational Phase Wildlife Monitoring Report. Burlington, Kansas. 48pp.Kansas Department of Wildlife and Parks. 2006. website at http://www.kdwp.state.ks.us Accessed December 20, 2006.Kansas Ornithological Society. 2003. Kansas Ornithological Society Birds of Kanas Checklist.

Tenth Edition. Available on web page http://wwwksbirds.org.

Accessed December 20, 2006.

Enclosure 8 to ET 07-0001 COFFEY COUNTY, KANSAS OFFICAL ZONING MAP A a C D E F a H I J K L m N 0 P 0 R a T V W X Y A E C 0 E F Q H I J K L m N 0 P Q A a T U V W X Y--t GRIýt1TU*.

AW) C-1t g1Cvi" C0 ftECAL C4DT.f-I i rmaM msION tTI. I-t UI ES r.ft CC11Y ROCK DAM___'il-NH-I RANUFCTINRwD mI OUX HDW WVISfl -a BNAD= 30 200NM Enclosure 9 of ET 07-0001 Table 1 Land Use Summary in the Vicinity of WCGS and Transmission Lines Percent County Land Use Categories Acres of Total Coffey Agricultural land 335,835 83.3 Cropland 193,375 48.0 Woodland 7,466 1.9 Rangeland (grazed) 123,296 30.6 Farmstead, ponds, wasteland, misc 11,698 2.9 Municipal, industrial, lakes, wildlife 67,202 16.7 Total 403,037 Lyon Agricultural land 493,853 90.7 Cropland 261,814 48.1 Woodland 10,642 1.9 Rangeland (grazed) 201,208 36.9 Farmstead, ponds, wasteland, misc 20,189 3.7 Municipal, industrial, lakes, wildlife 50,703 9.3 Total 544,556 Greenwood Agricultural land 594,368 81.5 Cropland 143,046 19.6 Woodland 10,746 1.5 Rangeland (grazed) 428,247 58.7 Farmstead, ponds, wasteland, misc 12,329 1.7 Municipal, industrial, lakes, wildlife 135,019 18.5 Total 729,387 Butler Agricultural land 701,202 76.7 Cropland 308,447 33.7 Woodland 10,978 1.2 Rangeland (grazed) 356,635 39.0 Farmstead, ponds, wasteland, misc 25,142 2.7 Municipal, industrial, lakes, wildlife 212,622 23.2 Total 913,824

Reference:

U. S. Department of Agriculture, National Agricultural Statistics Service. 2002. 2002 Census of Agriculture Volume 1 Chapter 2: Kansas County Level Data. USDA.Washington D. C. Also available online at www.nass.usda .qov/census/census02/volumel/ks.

Accessed 12/28/2006.

v t e Letter Sequence Response to RAI
TAC:MD3182, (Approved, Closed)
Initiation Request, Request, Request, Request, Request, Request, Request, Request, Request, Request Acceptance Supplement Administration Meeting, Meeting Questions 7 February 2007 9 April 2007 Answers 11 January 2007 Results Approval, Approval Other: ML070230546, ML071840188, ML072000312, ML072220449, ML072340443, ML072420149, ML080950177, ML081060277, ML081280749
MONTHYEAR2007-06-21 [Table View]

ML070180367

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SUMMARY

1.4 DESCRIPTION

1.5 REFERENCES

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2.1 INTRODUCTION

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3.1 INTRODUCTION

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4.1 INTRODUCTION

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5.1 INTRODUCTION

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Dear Mr. Logsdon:

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Dear Mr. Logsdon:

Description:

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SUMMARY

4.0 CONCLUSION

1.0 INTRODUCTION

4.0 CONCLUSION

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1.4 CONCLUSION

1.1 INTRODUCTION

1.4 CONCLUSION

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SUMMARY

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