Text

Sequoyah Nuclear Plant - Discharge Monitoring Report for May 2009
	ML091800413
Person / Time
Site:	Sequoyah
Issue date:	06/11/2009
From:	Cleary T P; Tennessee Valley Authority
To:	Cromer P; Office of Nuclear Reactor Regulation, State of TN, Dept of Environmental & Conservation, Div of Water Supply
References
	Download: ML091800413 (121)
	v • d • e

{{#Wiki_filter:j Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 June 11, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT -DISCHARGE MONITORING REPORT FOR MAY 2009 Enclosed is the May 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.Sincerely Timothy P. leary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)-Narn W ---- TVA-- SEQUOYAH-NUCLEAR PLANT Address P.O BOX 2000....- .i..NTEROFFICE SB-2A-SQN. SODDY -DAISY TN 37384 Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR." DISCHARGE MONITORING REPORT (DMR).(SUBR 01 )TN0026450 101 G -FINAL PERMIT NUMBER I DISCHARGE NUMBER DIFFUSER DISCHARGE MONITORING PERIOD EFFLUENT Form Approved.OMB No. 2040-0004 YEAR MO DAY YEAR MO I DAY From 09 05 01 To[ 09 05 31 NO DISCHARGE = ...NOTE: Read instructions before completinq this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OP TYPE~ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE

23.8 04 o 31 /31 MODELD CENTIGRADE MEASUREMENT 00010 Z .0 0 PEMIT 0. D5 .EG. C. SEE :K REQ INSTREAM MONITORING

.. -& .<"-DAILYMX-PERMIT TEMPERATURE, WATER DEG. SAMPLE * ** 40 0 04 0 31 I 31 RCORDR CENTIGRADE MEASUREMENT ý00010 1 0 0 .PERMIITrt REPORT~* >>~t DEG. C. SE ~CK FEQ: JEFFLUENT GROSS VALUE REQUIREMENT. PE'~ ALYM :RMIT jA.:V~ITEMP. DIFF. BETWEEN SAMP. & SAMPLE .2.1 04 0 31/31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 S 0 3 0ERMI

DEG. C. CNTI NCALTD EFFLUENT GROSS VALUE ~ REQ~iIREMENT1 .v# .< DAILY MX% UOUS~PH SAMPLE 7.5 7.9 12 0 7/31 GRAB MEASUREMENT 00400 1 0 0 P. -E RMIT: 6 0 9.0 SU WEEKLY ...GR...EFFLUENT GROSS VALUE MINIMEM .MAIMUM SOLIDS,TOTAL SUSPENDED SAMPLE
13 13 19 0 1 / 31 GRAB MEASUREMENT 19 " 00530 1 0 0 ,.. !PERMIT .,- ." EFLUNTGOS VLUREQUIREMENT 3 100MGL MNHLYGRB_________ROSSVALU MO AVG. %DAILY MX OIL AND GREASE SAMPLE ** <5 <5 j9 0 1 / 31 GRAB MEASUREMENT 00556 1 0 0i MON REQU~IREM.ENT r M AV .IL X EFFLUENT GROSS VALUE :MOAVG AILYEMX FLOW, IN CONDUIT OR THRU SAMPLE *1452 03 ** /31 RCORDR TREATMENT PLANT MEASUREMENT

.50050 1 0 0 - MGD " i****" ..N -.ONTIN O IEFFLUENT GROSS VALUE DAILY MVX :.< PdUQUS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Sequoyah Site Vice President I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel xoperly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system,.or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true,.accurate, and complete. I am aware that there are significant penalties for submitting false Principal Environmental Engineer TELEPHONE DATE 423 843-6700 09 06 09 SIG NATURE OF PRINCIPAL EXECUTIVE)FFICER OR AUTHORIZED AGENT.TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)No closed mode operation. The following information is included in an attachment:

1. CCW data 2. Veliger monitoring data AREA I CODE NUMBER YEAR MO I DAY EPA Form 3320-1 (REV 3/99) Previous editions may'be used Page 1 of 2 DMR Attachment CCW Data CCW TRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 05/13/2009

@ 1155 0.22 mg/I 05/14/2009 @ 1644 CLS TN EPA 8015 CCW CHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 05/13/2009 @ 1200 <0.10 mg/I 05/14/09 @ 1655 CLS TN EPA 8015____________ I ________________ i _____________ 1* I __________ Mean#ofMean# of NOTES: %Sample Date ZM/m3 % Settlers Water TempSample Date Asiatic r Temp. OCATION SUB, Gravid Asiatic COLLECTED BY.Mm ( SC) Clams/m3 (C) LOCATION Clam 11121/2008 11/24/2008 12/4/2008 12/8/2008 12/15/2008 12/22/2008 12/29/2008 01/09/2009 01/16/2009 01/22/2009 01/30/2009 02/06/2009 02/13/2009 02/20/2009 02/26/2009 03/06/2009 03/13/2009 03/27/2009 03/30/2009 04/09/2009 05/06/2009 05/12/2009 05/19/2009 05/27/2009 a 31 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 28 880 15091 3961 7696 0 0 0 0 0 0 0 0 0 0 0 0 0 0-0 0 0 0 0.0 24 0.3 2 0.8 12 11 10 9 9 9 9 8 7 7 9 6 11 9 9 8 10 14 13 14 19 20 20 22 11/16/2008 11/24/2008 12/04/2008 12/08/2008 12/15/2008 12/22/2008 12/29/2008 01/09/2009 01/16/2009 01/22/2009 01/30/2009 02/06/2009 02/13/2009 02/20/2009 02/26/2009 03/06/2009 03/13/2009 03/27/2009 03/30/2009 04/09/2009 05/06/2009 05/12/2009 05/19/20 09 05/27/2009 230 138 15 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 21 47.445 457 12 11 10 9 9 9 9 8 7 7 9 6 11 9 9 8 10 14 13 14 19.20 20 22 Inplant Inplant.Inplant Inplant Inplant Inplant Inplant Inplant Inplant Inplant Inplant Inplant Inplant Inplant Inplant Inplant Inplant-Inplant Inplant Inplant Inplant Inplant Inplant Inplant RCW RCW.RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Adcock / Bryant Adcock / Bryant Adcock / Bryant Adcock / Bryant Love Bryant Bryant Dick Adcock Dick Adcock Wayne Wright Dick Adcock Dick Adcock CNW CNW BJ CNW PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE Sa-2A-SQNk SODDY -DAISY TN 37384 Facilitv TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN; Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)(SUBR 01)TN0026450 101 G F -FINAL PERMIT NUMBER " DISCHARGE NUMBERI DIFFUSER DISCHARGE Form Approved.OMB No. 2040-0004 MONITORING PERIOD I1 EFFLUENT I I DYEARI MOi DAY I Y DAY N D j From1 09 05 1 To NO DISCHARGETo PARAMETER CHLORINE, TOTAL RESIDUAL 50060 1 0 0'EFFLUENT GROSS VALUE QUANTITY OR LOADING QUALITY OR CONCEN NU IO : RIeao instructuons oefore completing tnis urtm.ITRATION NO. FREQUENCY EX OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITI SAMPLE MEASUREMENT

****** *<0.018 0.042 19 MGIL 0 27 / 31DAYS GRAB PEFMIT~REQUIREMENT~
- - - - ~7 4;I..0.10 20.10 MOQAVG )INST MAX~~CALCTO ITEMPERATURE

-C, RATE OF .SAMPLE** 1 6******** 0 31/31 CALCTD CHANGE MEASUREMENT6 234 1 0 0 PERMIT ****;2 K DEG ~,~***.4CONTIN 'CALCTDQ JEFFLUENT GROSS VAILUE RDAILY MX C/HR UOUS SAMPLE MEASUREMENT SAMPLE" MEASUREMENT ~RE .. .4.%ýýL SAMPLE MEASUREMENT REQUIREMENT i~SAMPLE MEASUREMENT.IT ....... .,- ..REQUIREMENT 4.SAMPLE MEASUREMENT 4 -->PERýMITI<j i 7>j;.444'REQUIREMENT I-4 y 7*74..,tr.2.... -NAME/TITLE PRINCIPAL EXECUTIVE OFFrICER I Certify under penalty of law that this document and all attachments were prepared under my .tt direction or supervision in accordance with a system designed to assure that qualified personnel 4 w.,-k* C Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the Principal Environmental Engineer"oa Sinformation, the information submitted is , to the best of my knowledge and belief, true, *Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVI , _,information, including the possibility of fine and imprisonment for knowing violations: OFFICER OR AUTHORIZED AGENT E I TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)The following injections occured: 1. H-150M (max. calc. conc. was o.0446mg/L--limit 0.050mg/L)

2. H-150M (low detection level analytical method was <0.020mg/L--limit 0.050mg/L)

EPA Form 3320-1 (REV 3/99) Previous editions may be used .P age 2 of 2 PERMITTEE NAME/ADDRESS " (Include Facilitv Name/Location if Different) Name .TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON) SODDY -DAISY TN 37384 Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)DISCHARGE MONITORING REPORT (DMR)TN0026450 101T PERMIT NUMBER DISCHARGE NU MONITORIN PERI D YEAR MO DAY YEARI MO DAY From 09 05 01 To 09 105 31 MAJOR Form Approved.(SUBR 01) OMB No. 2040-0004 F -FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT NO DISCHARGE E: NOTE: Read instructions before completinq this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ** >100.0 23 0 1 / 180 COMPOS CERIODAPHNIA MEASUREMENT TRP3" 0 VALUE................E........45.2'*****,A, .PERCENT .......<MINIMUM777.7YiWi.~T J1C25 STATRE 7DAY CHR SAMPLE ** >100.0 2.0 1 /180 COMPOS PIMEPHALES MEASUREMENT 23ýTRP6C 1 ,0.,0 <PERMITs < 45.<2-:' PERCENT 1> SEE -OPO JEFFLUENT'GROSS VALUE I MIMUIREMPNRMI SAMPLE MEASUREMENT IREQUIREMENTW< 4.': SAMPLE MEASUREMENT PERMIT MREASUjIREýME N T SAMPLE MEASUREMENT -'PERMIT.4::< 4 REQUIREME!LT SAMPLE MEASUREMENT PER MIT ~.f .MEASUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my.- i ll TELEPHONE DATE direction or supervision in accordance'with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, __423 843-6700 09 06 09 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I CODE COMMENTS AND.EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)Toxicity was sampled 5/10/09 -5/15/09. Report is attached.EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 June 9, 2009 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, MAY, 2009 Per your request, I am only submitting an electronic copy of the subject report.The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101, samples collected May 10-15, showed no toxic effects to fathead minnows or daphnids. The resulting IC 2 5 values for both species were >100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.Call me at (256)386-2755 if you have any questions or comments following your review of the report.Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment): Sherrard, R. M., PSC I X-C Files, ER&TA, CEB I B-M SQN May 2009m TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: June 9, 2009 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA 2. County / State: Hamilton / Tennessee 3. NPDES Permit #: TN0026450 4. Type of Facility: Nuclear-Fueled Electric Generating Plant 5. Design Flow (MGD): 1,579 6. Receiving Stream: Tennessee River (TRM 483.6)7. 1Q1O: 3.491 8. Outfall Tested: 101 9. Dates Sampled: May 10-15, 2009 10. Average Flow on Days Sampled (MGD): 1426, 1443, 1448 11. Pertinent Site Conditions: H-150M was iniected into the ERCW B train beginning on 5/11/09 A 1220 through 5/14/09 (a- 1500.12. Test Dates: May 12-19, 2009 13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)Daphnids (Ceriodaphnia dubia)15. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephalespromelas:

UV treated Outfall 101 :11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0 16. Permit Limit Endpoint (%): Outfall 101: IC2 5 = 45.2%17. Test Results: Outfall 101: Pirmephales promelas: I__C_5100%Ceriodaphnia dubia: IC 2 5 > 100%UV treated Outfall 101: Pimephales promelas. IC 2 5 > 100%Page 1 of 107

18. Facility Contact: Ann Hurt.Phone #: (423) 843-6714 19. Consulting ITesting Lab: Environmental Testing Solutions, Inc.20. Lab Contact: Jim Sumner Phone #: (828) 350-9364 21. TVA Contact: Cynthia L. Russell Phone #: (256) 386-2755 22. Notes: Outfall 101 samples collected May 10-15, 2009, showed no toxic effects to fathead minnows or daphnids.

The resulting IC 2 5 values, for both species, were >100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Minnow survival was significantly reduced when exposed to intake samples during this study period.Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.Page 2 of 107 METHODS

SUMMARY

Samples: 1. Sampling Point: Outfall 101, Intake 2. Sample Type: Composite 3. Sample Information: Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET)ID Collected Received -) (rg/L) First Used By 101 05-10-09 0859 to 05-11-09 1510 1.8, 1.8' <0.10 05-12-09 0950 05-11-09 0759 05-13-09 0850 Intake 05-10-09 0933 to

05-12-09 0950 05-11-09 0833 05-11-09 1510 1.3 <0.100850 051ý09081 o05-14-09 08500 101 05-1-09 0821 to 05-13-09 1433 3.2, 2.0 t <0.10 05-14-09 0900 05-13-09 0721, 05-15-09 0853 05-12-09 0839 to 05-14-09 0900 Intake 05-13-09 0739 05-13-09 1433 2.1 <0.10 05-15-09 0853 05-16-09 0900 101 05-14-09 0828to 05-15-09 1428 2.3, 1.5' <0.10 05-17-09 0920 05-15-09 0728 05-18-09 0903 05-16-09 0900 Intake 05-14-09 0842to 05-15-09 1428 1.7. <0.10 05-17-09 0920 05-15-09 0742 05A18-109.0903
TRC -Total Residual Chlorine t Samples were collected in two 2.5 gallon cubitainers.

Temperature'was measured in each cubitainer upon arrival.4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0 0 C) in a warm water bath.Aliauots of Outfall 101 and Intake samtles were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.Page 3 of 107 Pimephales promelas Test Organisms: Ceriodaphnia dubia In-house Cultures<24-hours old 1. Source: 2. Age: Aquatox, Inc.20.33 hours old Test Method Summary: 1. Test Conditions: Static, Renewal Static, Renewal 2. Test Duration: 7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic 3. Control / Dilution Water: Moderately Hard Synthetic 4. Number of Replicates: 4 5. Organisms per Replicate: 10 6. Test Initiation: (Date/Time) Outfall 101 UV Treated Outfall 101 7. Test Termination: (Date/Time) Outfall 101 UV Treated Outfall 101 8. Test Temperature: Outfall 101: 10 1 05-12-09 1220 ET 05-12-09 1232 ET 05-19-09 1123 ET 05-19-09 1140 ET Mean = 24.7 0 C (24.2 -25.1 C)05-12-09 0950 ET 05-19-09 0854 ET Mean = 24.9°C (24.6 -25.2"C)Test Temperature: UV-Treated Outfall 101: Mean =24.7°C (24.3 -25.0 0 C)9. Physical / Chemic Measurements: al Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in. each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposuretestsolutions Were analyzed daily for pH and dissolved oxygen.Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).10. Statistics: Page 4 of 107 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.(Genus species) (Type / Duration)Conducted May 12 -19, 2009 using effluent from Outfall 10 .Test Percent Surviving Solutions (time interval used -days)(% Effluent) 1 2 3 4 5 6 7 Control 100 100 `100 100 100 100 100 11.3% 100 100 98 98 98 98 98 22.6% 100 100 100 100 100 98 .98 45.2% 100 100 100 100 98 98 98 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 98 98 98 98, Intake 100 100 90 68 60 53 50 Test Solutions Mean Dry Weight (mg)Tet Soluetis (replicate number)(% Effluent) 1 2 3 4 Mean Control 0.730 0.684 0.895 0.761 0.768 11.3% 0.753 0.655 0.723 0.717 0.712 22.6%. 0.654 0.723 0.673 0.760 0.703 45.2% 0.646 0.684 0.638 0.649 0.654 72.6% 0.665 0.632 0.656 0.603" 0.639 100.0% 0.723 0.604 0.700 0.641 0.667 Intake 0.212 .0.285 0.496 0.567 0.390 IC 2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*Permit Limit: 45.2%Permit Limit: 2.2 TUc 95'% Confidence Limits: Upper Limit: NA Lower Limit: NA*TUa I 00/LC 5 0: TUc = 100/IC 2 5 Page 5 of 107 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)2. Results of a Ceriodaphnia dubia Chronic/ 7:day Toxicity Test.(Genus species) (Type / Duration)Conducted May 12 -19, 2009 using effluent from Outfall 101.Percent Surviving Test (time interval used -days)Solutions 2 3 4 5 6 7_(% Effluent)Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6%' 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)(% Effluent) Data (replicate number)_%Eflunt 1 2 3 4 5 6 7 8 9 10 Mean Control 33 34 33 34 34 32 34 34 31 31 33.0 11.3% 33 36 32 32 36 33 33 34 33 32 33.4 22.6% 35 36 34 3'6 .32 33 35 34 33 33 34.1 45.2% 36 34 31 35 35 33 33 34 33 31 33.5 72.6% 37 37 36 32 35 32 31 34 34 33 34.1 100.0% 39 38 32 36 34 33 34 35 .34 31 34.6 IC 2 5 Value:- > 100% Calculated TU Estimates: < 1.0 TUc*Permit Limit: 45.2%Pennit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA*TUa = 100/LC 5 0: TUc = 100/IC 2 5 Page 6 of 107 --N TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.(Genus species) (Type / Duration)Conducted May 12 -19, 2009 using water from Intake Percent Surviving Test (time interval used -days Solutions 1 2 3 4 5 6 7 (% Effluent)- Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)Tet Soluetis Data (replicate number)(%Effluent) 1 2 3 4 5 6 7 ] 8 9 10 Mean Control 33 35 33 35 34 33 31 31 33 -33 33.1 Intake 39 35 41 36 35 38 37 36 37 34 36.8 IC 2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA*TUa = I00/LC 5 0: TUc = 100/IC 2 5 Page 7 of 107 TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.(Genus species) (Type / Duration)Conducted May 12 -19, 2009 using effluent from UV Treated Outfall 101.Test Percent Surviving Solutions (time interval used -days)(% Effluent) 1 2 3- 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 -100 100 100 98 45.2% 100 100 100 100 100 100 100 72.6% 100. 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 -100 100 100 100 Test Solutions Mean Dry Weight (mg)(% Effluent) .(replicate number)1 2 3 4 Mean Control 0.637 0.743 0.695 0.659 0.684 11.3% 0.775 0.852 0.756 0.772 0.789 22.6% 0.737 0.727 0.706 0.653 0.706 45.2% 0.693 0.722 0.683 0.756 0.714 72.6% 0.682 0.668' 0.665 0.722 0.684 100.0% 0.631 0.664 0.751 0.745 0.698 Intake 0.695 0.635 0.662 0.642 0.659 IC 2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*95% Confidence Limits: Upper Limit: NA Lower Limit: NA*TUa = 100/LC 5 0: TUc = 100/ 1C 2 5 A, REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)____REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)Species Date I Time I Duration I ToxicantI Results (1C 2 5)Pimephales promelas May 12 -19, 2009 1208 Ceriodaphnia dubia May 05 -12, 2009 0905 KC1 0.75 g/L NaC1 1.07 g/L Page 8 of 107 PHYSICAL/C ... .... i _ .,. .. .. .. .. .. ....... .. :.. .. ... .... .. .. ... .. .. .. :.................. I ........ I ............................................

......................................................

............................................ ........... ............. ............................. .. ... .......... HEMICALSUMVMARY W aE r IC Ae S U M M an .alu s. a d....e.f.r...es.................. N r...o.......... .................. .....P. _.................. .................. ................ .................... Water Chernisr Me yan ales and Ranges for Pimephales.prolas and Ceriodapnia dubia Test, Non-treated! S equoyahN uclr Plant (SQN) Outal 101 ............... performed May 12-19, 2009.Test Sample ID Temperature (C) Dissolved Ox ygen (r/L) pH (S.U.) Conductance Alkalinity Hardne s s Total Residual Initial Final Initial Final Initial Final (inihos/cm) (mg/L CaCO 3) (mg/L CaCO 3) Chlorine (mg/L)24.7. 24.6 7.6 7.4 7.55 7.25 321 61 89 C o n tr o ll ....... ....... ....... ."..... ....... ....... .... ... ....... ......... ..... ........ .....I. ....... ....... ....... .............. .............. ....... ....... ....... ............... .............. .............. .............. ...... ....... ....... .......24.7 24.8 24.5 24.8 7.4 -: 7.8 6.9 -.ý 7.5 7.32 -, 7.66 7.04 :-: 7.44 302 -: 338 60 -! 61 88"- 92 24.7 24.6 7.7 7.3 7.41 7.22 297 1 1 .3 % ......... .. .: .......... .... ..---.... ... .................... ....... ................. .......... 24.7 2. 24.8 24.2 -24.9 7.5 -: 8.0 6.9 "..7.5 7.26 7.50 7.01 -7.36 284 -..306 _____________ ____........ 24.8 24.5 7.7 7.2 7.40 7.20 280 22.6% ....... .. .. .. .. .... ....................... E2 .. 24.7: 24.8 -24.2 75 81. 6.8 7.5 726-: 7.49 7.03 7.34 268 287 24.8 24.6 7.7 7.2 7.37 7.19 243 S4 '5 .2 % ... .. ...... ..... -.- .. 2 4. .. ... ... .. ....... ... .. ... .... ...... -7 14...... ....... 1 ' .......... '......7. 3 -:' ........ ......... ..4...................... ........................ 5 '..................... ." ................ ....................... "..................................... .' .................. ..... ............................................. _____24.7

24.9 24.2 -24.8 7.6 8.0 6.8 74 .23 74 69 73 24 27 _____24.8 24.6 7.8 7.2 7.33 7.17 198 7 27 .. 6 % ..............

............ ...............................................

__24.7- 24.9 24.4 -24.8 77 7.9 6.8 75 7.18 74 697 7i 7......7734 189 ::%.205 24.8 24.6 -7.7 7.2 7.27 7.13 150 57 62 < 0.10 100"0°/ 24. 25.0 24.4- 24.9 7.7 .78 6.8 7.5 7.14 7.39 6.95 7.24 145 156 50 -65 60 -64 <"0. 10- <0,10 24.9 24.6 7.8 7.2 7.25 7.13 147 54 63 < 0.10 24.6 ::-: 25.1 24.4:: 24.7 7.7 7.9 6.8 7.5 7.11 7.39 6.97 7.25 143 153 52 ' " 57 60'"-: 64 <0.10:- <0.10 24.8 25.0 7.6 7.6 7.55 7.39 321 61 89 C o n tr o l ... : .. -..... .,.. ... :.: .. ....................

.... ..."" " "-' -................ .": ' ' " ...................... "- .--" ..-- .- ' "" .................. .. -"";...... ... .-T ' " ' " .." " ¢T ""... ............ ..' ...... ................. ........ .."..24l '"24.7-"25.0 24.8 -"25.2 7.4 -: 7.8 7.5 78 732 766 7.21 .7.47 302 338 60 -: 61 88"-'92 24.9 25.0 7.7 7.6 7.41 7.40 297 1 1 .3 %/ ....." .............. ....... ........ ..... ..." : ............... ... ... .. ........................ ."..................................... ............ ............................ ..................... 24.7 25.0 24.8 :-: 25.2 7.5 -: 8.0 7.4 7.8 7.26 7.50 7.22 -! 7.49 284 306.24.9 24.9 7.7 7.6 7.40 7.39 280_ 2 2 .6 % ... ....-....... ..-...... ...... :.... ....................... % ............ ........... .... .... ... .. ................

..............

..................... .:............ .: ..... ...... ...... ....24.7__ 25.I 24.8 -:25.2 7.5 -~8.1 7.4 7.7 7.26 7,49 7.22 7.48 268 287 _______24:9 25.0 7.7 7.6 7.37 7.38 243 4 .2% .....:, .............. ..... r"": ": ........ :.... .. ":......I...... "......... ... ........... ..................... ":.....................;" ....... ... ............................ ........ ....24.8 :4 25.1 24.8-: 25.2 7.6 80. 7.4 7.7 7.23 74 7.22 7.48 234 247 : .... _.....24.9 24.9 7.8 7.6 7.33 7.35 198 24.9 24.9 7.7 7.6 7.27 7.31 150 57 62 < 0.10 1 0 .% ......... ... ." : ............. ............. ...... .. I ..... ... :-........... ...... .. ....... i ........ .......... ......... ....... ........I ............ .. .. .......... -.............____24.8 25.1 24.7:- 25.1 7.7 7.8 7.5 7.7 7.14 739 7.20 7.38 145 156 50 ::ý65 60 ý- 64 <0.10-: <0.10 it. 24.9 24.9 7.8 7.6 7.25 7.28 147 54 63 < 0.10 I n a k e ... ...... ..... ......... .. ............ ........ ......... ....... ....... 1. ... ..... ... .......... ... ................. ......- ... .. ................. ............. .............. .. ............. .............. .. ....... ...... ..___24.8 -25.1 24.6- 25.1 7.7 79 75 77 711 739 709 7.35 143 153 52 57 60 -64 <0.101-ý <0.10 Overall temperature Average Minimum Maximum.. ... ............ ............ I ... ............. % ...i~ ............. .. ..i ~ ... ...... i........... ... ..... ... ..... ... .. ....... ... ..... ... .... ....... ... ........... .... .... .. ....... .. ....... ... .... .... ... .. .. .. ....... ... ....... .. .. ... ....... .. .. ... .. .Pim ephales promeias 24 24.2 25. 2 1 Ceriodaphnia dubia. 24.9 K 24.6 25.2 Page 9 of 107

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry.Mean Values and Ranges for Pimephalespromelas Tests, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed May 12-19, 2009... .. .. .. .. ... ........... ........ .......i .................. .... ............... i i ....... 7 ....i.............. .. ..... 1i ................ .... ............... ...................... i.............. ............ ...... .Test Sample ID Temperature (°C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance. Alkalinity .............. Hardness ......Initial Final Initial Final Initial Final (pimhos/cm) (mg/L CaCO 3) (mgIL CaCO 3)24.8 24.6 7.7 7.5 7.44 7.27 314 60 91 C 24.7 -24.9 24.4 :- 24.9 7.5 7.8 7.0 7.8 7.28 7.53 7.04 -. 7.44 296 334 59 61 .6 88 { 92 24.8 24.6 7.7 7.3 7.45 7.25 302 1 1 .3 %/ .. ..... ...... .i-: ..... ..... ...... ..... .. .......25 0 4 4 i- 4 .7 ..":- ............ .............. ..: i' : ............ ..... ....... 7 5 7 2 -::.............. .........

. ..... 7 .3.0 8 !:':'............

............ 732 2 :: ........ .... .............. 3 2 "{........ .............. ....... " .............. _____24.7 -. 25.0 24.4 -24.8 7.5 -~7.9 6.8 -~7.5 7.29 -.7.53 7.08 -7.38 282 -321_______ ______24.8 24.5 7.7 7.3 7.45 7.23 282 2 2 .6 % ..... ...!. ........... .... ..... ............. .. ... ...................... , .......... .. : ... ....... .. ........................ ..... .... ..... .......... ............ ....... .. ................... ... .. ... ..24.7 .-. 24.9 24.4 -: 24.7 7.5 -{ 7.9 6.8 7.5 7.29 -7.54 7.02 -7.39 264 299 .......... '" .......... _ ..24.8 24.5 7.8 7.2 7.42 7.21 246 4 5 2. % ............ , ...: ....... ........... ...........

..: ...... ............

... ....:... : .......... I... ............ ..:........... ................. ...:........... ............... ... .............. ............

.. ! ..............

_____24.7 -~24.9 24.3 -24.9 7.6 -~8.0 6.8 7.4 7.28 -7.51 70 7.8 231 258 24.8 24.6 7.8 7.2 7.38 7.20 200 24.7 -i 24.9 24.3 -: 24.8 7.6 8.0 6.8 7.5 7.23 -7.49 7.03 -: 7.36 189 207 _ '"_ _____100.0% 24.8 24.6 7.8 7.2 7.33 7.18 153 50 62 1 0 .% .........., ....... .... ...... ..... '" ............. ., ,.. .................. ... ................ " ........ .. ... ....... .. .. ... .. .. .. .... ... .. .... .. .. ::- 5 0! .. ... ... .24.7 -:25.0 24.3 24.8 7.6 -80 6.8 :75 7.19- 7.43 695- 7.30 147 159 44 54 60 64:": 64 24.9 24.6 78 7.2 7.30 7.14 149 53 62 I n t a k e 2 4 7..- ... .... ..... ... ... .. ... .... ... ...... ........ 5 ..7 .2.. 7 5.6 2 ....... ....... .....2 .7 252. 0 ..6.8 -7.5 7.15 7... ..43 6.8 7.2 141 i ...... 50 57.. 8 i... .. .. ... .. .. .... ......... -............... ................... .......... .............................

. ............

.. .............

I .......... ... :. .............. ........ ... : .............

... .. ........ .................

Ove rall temperature C(..) Average M.inim.um Maximum.Pimephalespromelas 24 7 24 3 25 0.. ... .... .......... .......... .... ..... ............................ .......... ....... .....: ...... .. ...... ....... .................... .. .. .............. ....... ...... .. ._ ........ .. ...... ........ .................

.............

...........

. .............

......... ......,...:.............. .............. .............. ... ............. ................................ Page 10 of 107

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected May 10-15, 2009, showed no toxic effects to fathead minnows or daphnids.

The resulting IC 2 5 values, for both species, were > 100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Minnow survival was significantly reduced when exposed to intake samples during this study period.Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah..2f Page 11 of 107 Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.2. Ceriodaphnia dubia Tests were conducted according to EPA-821 -R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL 1. Pimephales promelas None 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS 1. Pimephales promelas None 2. Ceriodaphnia dubia None Page 12 of 107 PHYSICAL AND CHEMICAL METHODS I. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments:

All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibraition and maintenance logbooks.3. Temperature was measured by SM 2550 B.4. Dissolved oxygen was measured by SM 4500 0 G.5. The pH was measured by SM 4500 H+ B.6. Conductance was measured by SM 2510 B.7. Alkalinity was measured by SM 2320 B.8. Total hardness was measured by SM 2340 C.9. Total residual chlorine was measured by ORION Electrode Method 97-70.QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted-according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as 1C 2 5 values in g/L KC1 or NaCl.2. Standard Toxicant:

Potassium Chloride (KC1 crystalline) for Pimephales promelas.Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.3. Dilution Water Used: Moderately hard synthetic water.4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.Page 13 of 107 REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).3. Standard Methods for the Examination of Water and Wastewater, 2 0 th Edition, 1998.4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).Page 14 of 107 Sequoyah Nuclear Plant Biomonitoring May 12-19, 2009, Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 107 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 10O1) Discharge Concentrations of.Chemicals Used to Control Mi crobiologic ally Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 15, 2009.Date :i:i:::::i::iii

iiiiiiiiiTo ebom ::ii!: C -0 i::i iiiCuprostat-

4.ii0. M i~iiii ! iiiii m g/L iiiiiiiii

{iiiiiiii m g/L ii!i{i iiii PF iiii ~ iii..............T .................... A z l ........................

::::::::::::::

0 3 1 / 9 8 ii} .............. .. .iiiii~ii 0 9 0 / 9 9 ~ i! ! ! ............ ............................. ........................ ! {ii iiii ..................................

...................:::::::

03/14/1998 .....~!!~i~ .......ii i iiiii: 0 0 9 :::iiiiiiiiiiii~iiiiii ~~~~~~... ........................... ............ i i -- -ii) ...... ..................... 0 9 / 2/ 9 9 ii~iii;ii~i{{ ii {i ii ii{{i~{ :::::::::::::::::i

: ::::::::::::::::::::

0 0 1 ::::::::::::::::::::

............. ~iiiiiiiiii 0 3 / 1 6 / 1 9 9 8 ::::::::::::::::::::

: : ::::::::::::::::1:

~~:::::::::

~:::: ........ ... .......................

I0 /2 / 1 9 9 {ii~~i}i~ii~~i i{ i....!...... .i~~i{!i}iii~~i!ii~!{i~ i~~iii{{{{}{{)ii~ii! .............................-............. ii~ 0 8 ]8/1 99 {}'i~{'{i{{{i~ii)i! i l 0 0 1 i ~ii~i~i{{i~ i ' i {;}i 0 024 ::i..................... 0 8 1 /1 9 9 0 .1 2 ii{{i ii {{ 0 0 2 4 ::i~i~i;:}[~i~ i~i~iii.... ..:::::::........::::::::...::::.....::: 08/20/1999 0 .023 i ~ ~ ...i .i.li.}.i.} 0 ..2. .....::::::::::::::::::::::::: 0 1 0 iii~iiiii~iii{i 08/21/1999~~~ ~~~ ..{{{{{{{{{{{{iii{i{}ii~ii~{ii~}iii{ .. 2 ........ ..i~~ii{ii .. .l ...... ....002..-:......iiiiii~ii~iiiiii 0 8 2 / 9 9 .2 {....... ..... il!i .0 0 4 :::::::::

: :::::::::::::::::::::::::::::::::::

0 3 1 / 1 9 Q , .. ... ................

0 8/ 4/1 99 ..... 0 .16 i ! .{.ii .i .. .2 i:i}:i i :ii:i: :iiii:::: iiiii:i!Page 16 of 107 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbi ol ogic ally Induced -Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 15, 2009 D at e ~ ~ ~ ~ ~ ~ ~ ..... }iiiiiiii ~ i i:ii~iii:i:i: T w r r m iiiiiiiiii C L -4 0 1 C u p ro sta t- i i 0 mg/L Xii~ii~~i~ iiiiiiii mgL iiiii! iiiii{ PF iiii~ iiiiiiii Copolymer

:! mg/L ii 01/31/2000

< 0.002 0iiii;i[ii2i~iiiiiii 009 iiiiiiiiiiiiiiiii}i 02 0 1 2 00 i}iiiiiii:iii~iiiiiii:i::iiii~iiiiil~i~~iil 0 .0 11 ii..[i.ii 0 .028 ........................... ~ ~ 02/03/2000 0.008 Xw,!~ i~ N ...00.. ...... 02/04/2000 0:::::::::::::::::::::::::009:::::::::::::::::::::::::::::::::: 0 06 N 0 09 ::::::::::

::::::::::

.......iiiiiii~i~iiiiii 0 2 0 /2 0 0 .0 :.......... ....... ...... .:::::................. ...... ..07 2 / 00 0 0 5 iiiiii~ii~ ~ iiiiii....... ..................... ........... i~iii~ .............. i ........... ........... ...0 7 /2 7 /2 0 0 0.... .. 1 ......................................... ............................. ........................ iiiiiiiii ii .) 0 .1 9:::::::::::::::::::::::::: ........................... 07/3 /20 0 0. 076 0.0 9 ............................. 07/26/2000 < 0.0057 0.019 07/27/20 0< 0.0141 ::: OS::::: ......::!l:: :::::....:::: 0.019 liiii N iiiiiiiiiiii!iiiii 12/11/2000 .... .. 14 ...::::::: : : :::::::: .2 i:i:i:~ N ......07/128/2000 ....0.0088 5-.3{i~!!{i 0.018 "j) ii i 072 91 /2000 i < 0 .0 208 ....... ..~i 0 .0 19 :::::::::::::::::::::::

!:! .. ..0 8 /0 17 / 2 0 0 0 .:!.:i.! ........i.ii:;!.:!.: < 0 .0 0 31 4 1 :i:~i:i:i::! 0 .0 1 9 ................ ................. 0 8 2 /202/1 1/200 0 ....... ...... ..ii~i~iiiiiii~ .0 7 ' iiiiiiiiiiN !iiiii~iiiii 0 .2 iiiiii ! ;~iiii ...................... 12/27/2000

,*'~

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.... .......... ii}i:i::i0 0 ..........iii;i i iiiiil......................... iiiiiiii iiiifii Page 17 of 107 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- May 15, 2009 Date .Towerbrom C 222 :: PCL-401 XL-363 Cuprostat-- H. IOM Wit/.. mgmg/L mg/L PF gL........ .. TRC hsP bt Copolymer DM D mg/L .Qut....... ... .. .. .. ........ ..:::: ...........

X:::::

.:: X:.::::::::::

.:.:::::.. .....!?.......Azole 01/02/2002N.."'.."" < 0.0079 ....2... ..... 0.02 0,006:X.-.: X...===================== .. ...:..... .............. ..." ' " " " " "::::::::::::::::::::::::: ..:.::::::::::::::: ` 01/03/2002 < 0.0042 0023 0.014 ...... -........... 08/06/2002 < 0.0014 iý' 002.""' 0.018 08/07/2002 < 0.01041 :::::::: : : :::::::::::00850. 1 08/09/2002 ,.iiliii~iili!!~!!~l!i;~~iiiiii~liiil <0.0 015 0. 111. 54!~ N :ii:li:iii:i 0.018 10/06/2002 ... <0.049..... 10/08/2002. ..0..2 ... ..0.0.8..... 10/11/2002~ 0.0042 0.4001 01/12/2003 .::0::0035: .......01/13/2006 .:.: :....................... ...... .01/14/2003. <0.011 ...~ 0 2 01/15/2003 <0.0063.. 0.02.. 020 ..01/06/2002 <0 0 3 iii'i 042 $.ýýýý 0 020.......... ......: :.. ........+ ': ................ i i ! i~ ~ ii i i i i i i ! : ! i i i ? i 01/17/20 3 <0 .0. ...-.....- ...-.-04/06/2003 < 0.0073.04 6 0 8 /0 5/2 0 0 2.. .... < 0 0 .1 8::::::::::

........................

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: : ::::::::::::::

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iiii:i:}i-Page 18 of 107 Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations' of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 15, 2009 D ate iiiiiiiiiiiii::i::iii::]T ow erb rom i ii P C L -4 0 1 C u rostat- ::H 4 3.0-M .i~ii ii{{iii~iii ii~{{!{i~ii~il~iiiii .... .... ...i::: iii~ i~i iii ............................. .... .. ..... .-.........................

A z o e 06/15/2003 ii< 0.0045 .................... ....{{i 0. 14 ::::::::::::::::::::::::::::: a i{{i 0 8 / 3 / 0 0 0 0 0 0 ... .. .... .......... .....................

< 0.0037 0.020

..............

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: ::::::: :::::::::

06/18/2 00 3

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::::::::::

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... ..... ,.... .......... .. ................ .. :.. ...Page 19 of 107 Table B-Il Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- May 15,2009 Date Sodrnm. Towerbrom PCi iii:i PCL-401 NL-363 Cuprostat-PF H-t30M Nalco Rt Haypo00eh W At mg/i ...... .mg/L igL mg/L .nL 7355]..... TRC .P hpa .Copolymer

D A : zole Qrt mg/i ............ -i ........ ...... .........

.............. Eo/Po 11/08/2004 .. <0192 0.030 ..... ..... ....11/09/2004 ..<00..2 0.016 .. .........11/107/2004 <0.0149874 0.0164A4 11/11/2004 iiiii~i!iiiii}~iii~~iiiii 0.014 3 OXl)i~~i i 49 iiii 0.017

02/0/205.. ............ <004 ~ 2 ... 0.010 02/07/2005 <0.0116 -W 0.010.... ... 0007..02/08/2005 <0.00023 ..0.010 02/1/200 ...... <0.004 .1 02/11/2005 .. 005 ..O ..... 0.010600 11/10/2004 0.....00.3149 ....... .......06/09/2005..... 0.01297 11/11/2004 ....0....1...... <0.01849 07/19/2005

:0.0263 : : ... 0.0 ........07/121/2005

..0024...... 00 10/30/2005 .. <0.0068 .0. -4 02/01/2005 0.0042 0.0100 ..... ......07................. ..........

: : : :: :....-...,....,.......................

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..:.:. ...MSW 101 mg/L Phosphate Page 22 of 107 Sequoyah Nuclear Plant Biomonitoring May 12-19, 2009 Appendix C.Chain of Custody Records and Toxicity Test Bench Sheets Page 23 of 107 C-4 BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Cliemt: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity "351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comments-/ 1 J),f- 'Chevy Wilas NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell : v Adam Deiml-in Collected By: Chevy Williams, Ben Mitchell, Adam Deimling Fax: 828-350-9368 Metals Analyzed. Samples remained on ice through out sampling and transport to lab.Field Identification/ Grab/Comp. Collection Date/Time Container Flow Sample Description Number & MGD Rain Event?Volume (Mark as Appropriate) Laboratory Use Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear.(EST) .. Inches Number Temp. ance SQN-101-TOX Comp 05/10/09-05/11/09 0859- 2(2.5gal) ,, Tl i,0 1 .j ' ... L '\So SQN-INT-TOX Comp 05/10/09-05/11/09 0933- 1 (2.5 gal) NA , 0,0( 90S1-0 e l0 , ~~0833 ..Sample Custody -Fill In From Top Down --es0- o , Relinquished By (Signature): Date/Time Received By (Signature): .d%(me Chevy Wil 05/11/09 Sonic Delivery 05/11/09 Sonic Delivery 05/11/09 ETS 05/11/09_ __ __// E- 'Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6 0 C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. cr~u~I 0 BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 I q-1 Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number: .TN0026450 Collected By: Chevy Williams, Ben Mitchell, Adam Deimling Environmental Testing Solution, Inc.351 Depot Street.Asheville, NC 28801 Phone: 828-350-9364 Delivered By (Circle One): FedEx UPS Bus Client Other (specify): Sonic Delivery General Commen Chevy Williams:/ [,1 1 J Ben Mitchell: Adam Deimhng: _ _ _ _ _ _Metals Analyzed. Samples remained on ice through out sampling and transport to lab.Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Container Flow 'x ..+/-: 5A4 4 Li Sample Description Number & MGD Rain Event? LaboratoryUse Volume (Mark as Appropriate) .a.Collected __._Date Time Yes if Yes, No Trace ETS Log Arrival By Time Appear.(EST) InhsNumber Temp.nc SQN-101-TOX Comp 05/12/09-05/13/09 0821 -2 (2.5gal) L2-2 1 0721 / "" SQN-INT-TOX Comp 05/12/09-05/13/09 0839- 1 (2.5 gal) NA 3 .,\ ".Sample Custody -Fill In From Top Down -4 d A- *Relinquished By (Signature): Date/Time Received By (Signature): -Date/Time Chevy Williams/ .-05/13/09 /( -3!5 '6S Sonic Delivery 05/13/09 SonicDelivery 4~ to*A3 A1 , Sonic Delivery 05/13/09 ETS 05/13/09 Lo-4'I J.Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6*C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. ii BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of I.3 -0 Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Environmental Testing Solution, Inc.351 Depot Street.Asheville, NC 28801 Phone: 828-35,0-9364 FedEx UPS Bus Client Delivered By (Circle One): Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Other (specify): Sonic Delivery General Commen ts/t./4 .Chevy Williams.Ben Mitchell : Adam Deimling: Metals samples taken. Samples remained on ice through out sampling and transport to labI Collected By Chevy Williams, Ben Mitchell, Adam Deimling Fax: 828-350-93-68 Field Identification / Grab/Comp. Collection Date/Time Container Flow eCULP Volume (Mark as Appropriate) PfAi-Collected " __ .. .._* ....__Date Time Yes If Yes, No Trace IETS Log Arivl By Time Appear-__ -, Inches Number Temp, .'ance SQN-101-TOX Comp 05/14/09-05/15/09 0828- 2 (2.5gal) is-f " oq0 .LI.s".. -...0728 1449-3.SQN-INT-TOX Comp 05/14/09-05/15/09 0842- 1 (2.5 gal) NA " t .. ...0742 "ý I " 6 _ , [ -' ",r I r " 'Sample Custody -Fill In From Top.Down W o _ _ _ -._-Relinquished By (Signature): Date/Time Received By (Signature): .D~/Tine Chevy W 05/15/09 Sonic Delivery 05/15/09 Sonic Delive 05/15/09 ETS 05/15/09 SonicDelivery!Q; Z r f" K 1'_Z --Instructions: Clients should fill in all areas except those in thle "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be markedfor Saturday delivery or they will not arrive until the following Monday.

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)Species: Pimephales promelas Page 1 of 6 Client: TVA Facility:

Seuo-yah Nuclear Plant NPDES #: TN 0026450 Project #: :,4ýAA County: Hamilton Treatment: Non-treated Outfall: 101 Dilution prearation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information: Organism age: 2o. -15 AQuA OCb Randomizing template: "ILOg Date and times organisms 0.%- 0o% 16,0 Incubator number and were born between: shelf location: _-___Organism source: N-,-f- %'Mty\ Pe 0,- 1v-(A Artemia CHM number: CARI4'4 I Transfer bowl information: pH = SU Temperature = 'C Date / Time in: .oG Ir-z-oc>-.Date/ Time out: os.1m.i i,.L7o Average transfer volume: Total drying time: -- .tIS Au 0. Initial oven temperature: to '(........ _ _ _ _ __ __ Final oven temperature: , Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, renewal, Sample MHSW or termination numbers used batch used Time Analyst Time Analyst Time Analyst 0 o _____ iAoo -0 v0 .o 177- C .OSIk-Go -0LO-CPP I__ os-IS-0'1 0&00 A.~ '4Po &4 IiA 0j.1aw -o -kO os-aAA 2 0---A at A 11 3C k I _ý ORS 1,6. 0 OR 6ý- a 1- 6 %3 0. s-0% R ( ? 6 L 7 IT, 0ois6*IZo-Or 6 0oS-Is- 01A oto,0 I-LBO A~kb.1e6v*"51o$.tL-0i 7 I~ 12~ __________ _______Control information: Acceptance criteria Summary of test endpoints: % Mortality: I " <20% 7-day LC 5 0 1 ! Average weight per initial larvae: ,1 te " NOEC 7.7.6%Average weight per surviving larvae: 0 .1 %, I > 0.25 mg/larvae LOEC A,1, ChV IC 2 5[ Page 27 of 107 6 0 J'ETS£nwIw IlEOtI fln~SOiu bT% K Page 2 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant -Non-treated Date: -%'.- ooI Survival and Growth Data Day CONTROL 11.3% 22.6%A B C D E F G H I J K L 0i 0/( (0 I /0 (0 /0 to to /0 /0 to 10 1 (00 I6 )0 t 0 /0 /0 /0 /0 /( to 2 10 t/Q to IO to / to I t to 4 )a 10 / 0 10 o /0 /0 tO /0 to 1D 5 ID o Ib 10 /i 1b /0 /0 /0 /0 /0 6 to ID IQ) to 11() to _(3 to toI cI /0 7 It I0 C O (0 (0 (0 t q0 0t 10 at " 10 A = Pan weight (mg) liAt Tray color code:: 1it j 13 .15 1;A0 K so 11.40 5,69 j. 5o .11 ISý.13 13-.4 Analyst: fn, l r ""-")Date: 0 5" -_ __-_ _ __B = Pan + Larvae weight Analyst: NA ..IO Date: r-05 -C = Larvae weight (mg)=B-A Weight per initial number of larvae (mg)= C / Initial number of larvae Q~)N A I, t,.C" Average :Percent weight per reduction initial from control number of (%)larvae (ag)c.1?- 1.V7." 0.1 s.Comment codes: c = clear, d =dead, fg = fungus, k = killed, m = missing, sk sick, sm = unusually small, lg unusually large, d&r = decanted and returned, w = wounded.Calculations and data reviewed: Comments: Page 28 of 107 E TS Page 3 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant -Non-treated Date: 05-12-cA Survival and Growth Data Day 45.2% 72.6% 100%M N 0 P Q R S T U V W X 0 0"_ _ 1( /0 (0 /10 1 /0 10 10 103/0 to I U I IO t C) to to tO C) to to /O 2 1 C) I t (o /to 10 lo I0 o 6 /0 to, I )3 10 1 (0 1(3 I10 0 /03 10 10 I0 4 1 l./0 10 Io 0 10 to /0 t0 to to 50 (0 IC) /O /0 IO /O 10 q 10 tO 6 Io 10 10 10 /t to Itb to / 9 i) 10 7 ISM to 10 T to 16 /) cl /0 t(A = Pan weight (mg)Tray color code:: P!l Y3rJ3L l.S Analyst: ,ALlt r. ,.3.- 0 I4.o ." 3 .gt .jjg 1 I4: l3 Date: 5- '- _ __-0_B = Pan + Larvae weight (mg) aD,3ý o,.81 X 08 P.S"7 A,03- A 0 ý, X..33 U. 10 Analyst: 09P -" " Date: A.Q-. i .*C = Larvae weight (mg)= B -A 6 -4 1-. 6, q t.0" U bfr a (1 4 6 ,. 6 6. 5 L, .6 6,-0-5 " .t .%4 1 Weight per initial numberf', A 0 of larvae (mg) 1\' '= C / Initial num ber of larvae N" 4" \ -V, * \. ..-w -. ---- t- -Average Percent weight per reduction -17 initial from control 1 .*number of (%)larvae (mg)Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk -sick, sm = unusually small, lg = unusually large, d&r -decanted and returned, w = wounded.Calculations and data reviewed: A Comments: Page 29 of 107 lmETS E-wIM~nmp ft. blg soU~ I , Page 4 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant -Non-treated Date: OS-i'2-0 7-Survival and Growth Data Day 100% Intake Y Z AA BB 0 0 /0 ( t0 "/0_______ /L /0 /0 /0 2 1 0 (10I 3 4 qL4A1 1,~ I 5 3 d~ V'jF4 1-6 AF 7 1AFi A = Pan weight (mg), Tray color code:: ' I'-jti. -i.'t Analyst: _U " -_Date: b6 '- 1-8 41 B = Pan + Larvae weight (rag) lSq ql Analyst: _ ) I Date: D57 -___ -__C = Larvae weight (mag)=B-A Weight per initial number of larvae (mg) q t% 0 \= C / Initial number of larvae Average Percent weight per reduction initial from control o. +1 .2 7 number of (%)larvae (nmg)Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.Calculations and data reviewed: Com m ents: , .-vt t3 -Ae I.Fikde #"M I NT AYP_ b OI e -Mt MT i N I~, .C..-_ba4 R~J6U9 feIse~ejT-ný IeAtý LAOvAE. 4U 1 Page 30 of 107 --i-Environmental Testing Solutions, Inc.TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 12-19, 2009 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 5444 Reveiwed by: -Not for Compliance Assessment, Internal Laboratory QC ___ra %) Pplicau Initialt nmbet of Finial nuimber of A -Fan wright B Psn + La l L wrn i cght (mg) Weight/Swrviving M s.a eightl Cafdentao rada a Weigillt niitial taiber Mlea ns airvivi Mcn ar weight I t of Pe rc et ead on hr-la ua e la rae (rg) waigit (mg) -A-B Ofambe lrolanaermeJ 8,rsivinganmreeef 'ai' ' -d hlan atia j 1%) g.Initiual .beraof variat u..oni. rra ( %n )lavat (J." heva (tR) 1 A 10 t0 14.87 22.17 .7.30 0.730 0.730 Control a t0 t0 13 86 20.70 6.84 0.684 0768 11.8 0.694 100.0 0.768 11.8 Not applicable C 10 t0 15.01 23.96 8.95 0.895 0.895 D 10 10 12,40 20.01 7.61 0.761 0,761 E 10 t0 14.50 22.03 7.53 0.753 0.753 11.3% F 10 9 1370 20.25 6.55 0.728 0.730 2.2 0.655 97.5 0.712 5.8 7.2 G 10 10 13.68 20.91 7.23 0.723 0.723 IL 10 10 14.50 21.67 7.17 0.717 0.717 1 10 t0 1391 20.45 6.54 0,654 0.654 22.6% 1o 9 13.27 20.50 7.23 0.803 0.723 9.8 0.723 97.5 0.703 6.9 8.5 K 10 t0 15.13 21.86 6.73 0.673 0.673 L 10 10 13.90 21.50 7.60 0.760 .0.760 M 10 10 .13.89 20.35 6A6 0.646 0.646 45.2% N 10 I10 13.97 20.81 6.84 0.684 0672 4.5 0.684 9.5 0.654 3.1 14.1 0 10 9 13.70 20.08 6.38 0.709 0.638 P 10 10 14.08 20:57 6.49 0.6.49 0.649 Q 0 .to0 13.72 _20.37 6.65 0.665 0.665 72R 10 10 1575 22.07 6.32 0.632 00.639 4.3 &632 100.0 0.639 43 16.7 S _0 10 14.24 20.80 6.56 0.656 0.656 T 0" 10 14,28 203) 6.03 0,603 0.603 U to 10 )4.94 22.17 7.23 0.723 0.723 09,1 6.04 0.671 0.684 5.2 .0604 97.5 0.667 , 8.2 13.1 100% W 10 10 1433 2333. 7.00 0.700 0.700 X 10 10 )369 20A0 6.41 0.641 0.641 Y 1O 3 13.79 15.91 2.12 0.707 0,212 100% Intake _ __0 4 14!14 16.99 2.85 0.71 0.764 8.3 085.50.0 0.390 43.2 49.2 AA 6 14.85 19.81 496 0.827 0.496 8B 10_ 7 13.49 19.16 5,67 0810 N0.567 Outfall 101: Daunnett's MSD value: PMSD: Itaake: Dunnett's MSD value: PMSD: 0.0890 11.6 0.1860 24.2 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.Lower PMSD bound determined by USEPA (l0th percentile) = 12%.Upper PMSD bound determined by USEPA (90th percentile) = 30%.Lower and upper PMSD bouinds ware determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET lnterlaboratory Variability Study (USEPA. 2001 a USEPA, 2001b).USEPA. 2001 a, 2 0 0 1b. Final Report: Interlaboratory Variability Study ofEPAShort-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-04 and EPA-821-B-0I-005. US Environmental Protection Agency, Cincinnati, OH 0 S"ETS I Environmental Testing Solutions, Inc.TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 12-19, 2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: TVA/ Sequoyah Nuclear Plant, Outfall 101 End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. .Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-% 1 2 3 4 D-Control 0.7300 0.6840 0.8950 0.7610 11.3 0.7530 0.6550 0.7230 .0.7170 22.6 0.6540 0.7230 0.6730 0.7600 45.2 0.6460 0.6840 0.6380 0.6490 72.6 0.6650 0.6320 0.6560 0.6030 100 0.7230 0.6040 0.7000 0.6410 Intake 0.2120 0.2850 0.4960 0.5670 Transform: Untransformed I-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7675 1.0000 0.7675 0.6840 0.8950 11.817 A4 0.7675 1.0000 11.3 0.7120 0.9277 0.7120 0.6550 0.7530 5.777 4 1.502 2.410 0.0890 0.7120 0.9277 22.6 0.7025 0.9153 0.7025 0.6540 0.7600 .6.851 4 1.760 2.410 0.0890 0.7025 0.9153*45.2 0.6543 .0.8524 0.6543 0.6380 0.6840 .3.113 4 3.066 2,410 0.0890 0.6543 0.8524*72.6 0.6390 0.8326 0.6390 0.6030 0.6650 4.343 4 3.479 2.410 0.0890 0.6530 0.8508*100 0.6670 0.8691 0.6670 0,6040 0.7230 8.152 4 2.721 2.410 0.0890 0.6530 0.8508 Intake 0.3900 0.5081 0,3900 0.2120 0.5670 43.230 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97087646 0.884 0.63537989 1.21808683 Bartlett's Test indicates equal variances (p = 0.23) 6.92620468 .15.0862722 Hypothesis Test (I-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 22.6 45.2 31.9612265 4.42477876 0.08902763 0.11599692 0.00884297 0.00272926 0.0292745 5, 18'Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 95% CL(Exp) .Skew IC05* 7.813 ICI0 28.104 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100* indicates IC estimate less than the lowest concentration sqnIO1_05-12-O9data 0 0-4 TVA / Sequoyah Nuclear Plant, Outfall 101 -Intake Non-treated May 12-19, 2009 S 0 0 Environmental Testing Solutions, Inc.Statistical Analyses-Larval Fish Growth and Survival Test-7 Day Survival Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 -Intake End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-82 I-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-% 1 2 3 4 D-Control 1.0000 1.0000 1,0000 1.0000 11.3 1.0000 0.9000 1.0000 1.0000 22.6 1.0000 0.9000 1.0000 1.0000 45.2 1.0000 1.0000 0.9000 1.0000 72.6 1.0000 1.0000 1.0000 1.0000 100 1.0000 .0.9000 1.0000 1.0000 Intake 0.3000 0.4000 0.6000 0.7000 Transform: Arcsin Square Root I-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD" D-Control 1.0000 1.0000 1.4120 1.4120 1,4120 0.000 4 11.3 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 22.6 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 45.2. 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 72.6 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 100 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4*Intake 0.5000 0.5000 0.7854 0.5796 0.9912 23.814 4 6.701 2.353 0.2201 Auxiliary Tests Statistic Critical Skew Kurt Shapi'ro-Wilk's Test indicates normal distribution (p > 0.01) 0.93208891 0.749 3.38354E-16 0.88161324 Equality of variance cannot be confirmed Hypothesis Test (I-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Heteroscedastic~t Test indicates significant differences 0.11179766 0.11466427 0.78530011 0.01749093 5.4E-04 1,6 Treatments vs D-Control sqnIO0_O5-12-O9data TVA / Sequoyah Nuclear Plant, Outfall 101 -Intake Non-treated E'., Environmental Testing Solutions, Inc.May 12-19,2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 -Intake End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWC1-R-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-% 1 2 3 4 D-Control 0.7300 0.6840 0.8950 0.7610 11.3 0.7530 0.6550 0.7230 0.7170 22.6 0.6540 0.7230 0.6730 0.7600 45.2 0.6460 0.6840 0.6380 0.6490 72.6 0.6650 0.6320 .0.6560 0.6030 100 0.7230 0.6040 0.7000 0.6410 Intake 0.2120 0.2850 0.4960 0.5670 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.7675 1.0000 0.7675 0.6.840 0.8950 11.817 4 11.3 0.7120 0.9277 0.7120 0.6550 0.7530 5.777 4 22.6 0.7025 0.9153 0.7025 0.6540 0.7600 6.851 4 45.2 0.6543 0.8524 0.6543 0.6380 0.6840 3.113 4 72.6 0.6390 0.8326 0.6390 0.6030 0.6650 4.343 4 100 0.6670 0.8691 0.6670 0.6040 0.7230 8.152 4*Intake 0.3900 0.5081 0.3900. 0.2120 0.5670 43.230 4 3.944 1.943 0.1860 Auxiliary Tests Statistic Critical .Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 001) 0.94991392 0.749 0.13317371 -1.3590747 F-Test indicates equal variances (p 0.34) 3.45560646 47.4672279 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.18600391 0.2423.5037 0.2850125-0.01832517 0.00759199 1,6 Treatments vs D-Control sqn101_O5-12-O9data TVA / Sequoyah Nuclear Plant, Outfall 101 -Non-treated May 12-19, 2009 Environmental Testing Solutions, Inc.Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number: 5444 Reviewed by: Concentration Parameter Da 0 Day 1 Da Da 3 Da4Da 54 1_.Da 6________ ________________ Initial Final Initial IFinal Initial Fnl Initial Final Initial IFinal IInitial inl Initial IFinal-Initia Final pH (SUJ) 7.32 7.28 7.601 7.15 7.65 713 7.58 7.30 7.601 7.441 7.43 7.38 7.661 7.04 DO (mg/L) .7.5 .7.4 7.61 7.4 7.4 7.5 7.7 7.5 7.81 7.51 7.6 7.3 7T41 6.9 Conductivity (pmhos/cm) 317 302 326 Control Alkalinity (mg/L CaCO 3) 61 616 Hardness (mg/L CaCO 3) 88 Temperature CC) 8 8 8 24.5 24.7 24.5 24.7 24.5 24.7 245 247 24.5 24.8 24.6 PH (SIJ) 7.47 7.21 7.40 7.22 7.39 7.121 7.26 7.30 741 7.36 7:50 7.33 7.42 7.01 DO (mg/L) 75 73 76 72 7.6 7.3 7.8 7:5 80 74 77 7.4 75 6.9 11.3% Conductivity (pmhos/cm) 293 292 284 298 304 300 306 Temperature (C) 24.8 24.9 24.8 24.5 24.7 24.5 24.7 24.7 247 242 247 24.5 24.8 24.6 PH (SU) 747 7.18 741 715 7.37 7.09 726 7.30 740 734 749 7.33 7.41 7.03 2.% DO( L) 7.5 7.1 7.71 7.1 7.6 7.3 7.81 7.5 8.1. 7.41 7.7 .7.4 7.5 6.8 22.6% Conductivity (pmhostcm) 28 277 268 279 286 280 287 TemperatureC) 248 251 248 24.3 247 24.3 247 24.7 248 242 24.8 24.4 248 24.5 pH(SU) 747 719 741 715 731 7.09 723 7.30 736 735 7.45 7.28 7.37 6.99 DO(mg/L) 77 71 77 71 76 72 78 7.4 80 74 7.7 7.4 7.6 6.8 45.2% Conductivity (pmhos/cm) 247 243 234M 01947 243 246 Temprerature C) 248 248 248 247 .247 242 247 248 249 245 248 24.4 24.8 24.5 PH (SU) 7.46 7.12 7.35 7.12 7.27 7.09 7.18 7.29 7.30 7.34 7.42 7.23 7.32 6.97 DO (m L) 7.7 7.2 7.7 7.1 7.7 7.3 7.9 7.5 7.8 7.4 7.7 7.4 7.8 6.8 7./0 Conductivity (junhos/cm). 205 202' _____ _189196___ 196 199________Temperature ('C) 24.8 24..8 24.9 24.5 24.7 24.6 24.7 24.6 24.9 24.5 24.8 24.4 24.9 24.6 pH (SU) 7.391 7.131 7.30 7.12 7.191 7.041 7.141 7.231 7.24 7.23 7.361 7.241 7.26 6..951 DO (mgL) 7.71 7.11 7.7, 7.1. 7.71 7.51 7.81 7.41 .7.8, 7.3 7.71 7.31 7.81 6.86 Conductivity (pinhos/m) 1145 214 100% Alkalinity (mg/L CaCO3) 57 50 Hardness (mg/L CaCO3) 6 60 Total Residual Chlorine (mg1L) <0.10 <01 Temperature ('C) 24.8 24.9 24.9 24.5 24.71 24.4. 24.7 24.6 25.0 24.6 24.9 24.6 24.9 24.6 pH (SUJ) .7.39 7.15 7.3011 7.05 7.171 7.011 7.111 7.24 .7.221 7.25 7.341 7.231 7.25 69 DO (mg/L) .7.8 7.2 7.71 .7.1 7.81 7.41 7.9 7.5 7.81 7.31 7.71 7.41 7.86.Conductivity (I~mhos/cm) 153 143 48 .7 100% intake Alkalinity (ng/L CaCO 3) 54 Hardness (nsg/L CaCO3) (6 Total Residual Chlorine (mg/L) <0. <0.10_Temperature ('C) 24 24.9 24.6 24.9 24.6 24.6 24.7 25.11. 24.5 24.8 24.4 24.9 24.5 EDTS Page 5 of 6 Species: Pimephales promelas Client: TVA/ Sequoyah Nuclear Plant -Non-treated Daily Chemistry: Date: o5-aL-00%Day Concentration Parameter CONTROL pH (S.U.) -4.32 :51 Conductivity T)(j-mhos/cm) Alkalinity (mg CaCO 3/L) , Hardness (mg CaCO3VL)________ Temperature ('C) j. ~ i.- ~ L~S -A1 'i.pH (S.U.) Y 11.3% DO (mg[L)Conductivity 3 (jimhos/cm) Temperature (°C) L.q. k [ iA., "i pH (S.U.)3.t'T34 22.6% DO (m.gf) 7-7 .Conductivity (_Wmnhos/cm) 0 Temperature (°C) t.4. , "..- * .- "L ,/, pH (S.U.) L-4 41 45.2% DO (mg/b) 11 Conductivity _ _ _ __.. 9 (j~imos/crn) _________Temperature ('C) i~' ___ 1 1 t pH (S.U.) T______72.6% DO (mg/b)Conductivity (jimhoslcm) Temperature ('C) 1I --IAq1 PH (S.U.)3 100% DO (mgf/ *-4)Conductivity (pmnhos/cm) Alkalinity (mg CaCO 3/L)Hardness .6oq (mg CaCo 3 nL)TR chlorine (mgfL) .4.001__________Temperature ('C) i' ?L .i.*.I PH (S.U.)100% Intake DO (mg/b) 7 Conductivity) Alkalinity (mg CaCO 3/L) S Hardness (mg CaCOJ/L)TR chlorine (mgIL) <4.6 .0. 10________Temperature ('C) 2-1. zN 0 z 9 N Initial Final I Initial 11 Final I Initial 11 Final Page 36 of 107 i~TS~ En~,o MT~5ng5dw~ h,~.Page 6 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant -Non-treated Date: 05- a-001 I I Dav I ID I 3 4 .I -5 I 6 I U -Analyst I Concen- Parameter traili I _________CONTROL pH (S.U.)DO (m-/L)4 --Conductivity (4mhos/cm) Alkalinity (mg CaCO 3/L)1.(00. 4ý+3 33k

zq, I 4..1 1 t-.55 1 4-466I"_3 18 I 1 -3 lk_-4 B -L Hardness (me CaCO,/L)4 Temuerature (MC)9 --4~-ll DH (S.U.)ý im ir--4,3(o 1. -205 1 :f33 1-.'I I "I~L 11.3%DO (maL)~a~o Iii Conductivity I (A nib 0 M)8.0 1114 11-.4 1 -45 1V A 309 ot 3oco 0.-1 P"W M, A 1 P". M, Temperature

(°C)LA. I -U .1 a --.-..-.l = -DH (S.U.)0 1 ;7Z79 MI 9 -1)3,4 1"-,4q II _-4.33 1 L-1 II -.O- I 4 ~I H I *22.6%DO (m,/L)Conductivity (limbos/m) Te..n.erat.... (0-C I T emperature ('Q pH (S.U.)1.?0 I ~47L~L 3 I~Y[aS I i~ II ~.2iL~A~J 4-i 45.2%DO (mg/L)Conductivity (grmhos/cm) Iq 2_q 7 l° I , ,*I I I Itur (C Temperature ('Q-- i I pH (S.U.)~~7I *i.2q I ~l-7 4~A~)1 ~4 I ~I4d~ II 1.~-3 I i.~; IK~IAIJ-4,93L 72.6%DO (mg/L)Conductivity (Amhos/cm) 2~7 -1_4j t C (4 I A4 199-2A. t LqA %qA -L4 .LA Temperature ('C)DH (S.U.).1.1 7-iaI '3 Ih1~ ~A 14 i -4.tAL I! O I 100%DO (mi/L)4 -Conductivity Lcm)Alkalinity Hardness (Mg CaC03/L) I TR Chlorine (mgfL)Temperature (°C)N -~ ~ _ 1 4.9 VA. 6 i .,(I t., 9 I WH (S.U.)I 4,1/ 11 7. I t770 1 X3 I 1 .'1 IlI 1 -.1q I 3 1 II ,GA, I 100%Intake DO (mr/L)I II .* -'-~-.-Conductivity Alkalinity (mg CaCO3/L)Hardness (rag CaCO3/L)TR chlorine (mg/L)Temperature ('C)I +'-- .I I -- U .I I 0-,g II .1.Lt I ~ II ~ I T IP 't It 11 cR -N;Initial F-i n7l r"'i, -F Initial Final Final I Initial 17 Page 37 of 107 a 0~ on, T..Ihg5o~n~ no.Page I of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)Species: Ceriodaphnia dubia Client: TVA Facility: Sequoyah Nuclear Plant NPDES #: TN 0026450 Project #: .;,A'A County: Hamilton Treatment: Non-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism source information: Test information:.Organism age: I< 24-hours old Randomizing template color: istoe_Date and times organisms were born Os- I'L.-cf&\ o01-b -To M-AO. Incubator number and shelf between: location: Culture board: O5-06,-C A Replicate number: 1 12 13 4 15 16 17 a 9 10 yWVT batch: Culture board cup number: 3' 5 ID SI t'\-I "LV L 2 "LS OITL-CA Transferbowl information: pH = \.q'6 SU Temperaturen

A.. C Selenastrum batch: O TM Daily renewal information:

Day Date Test initiation, MHSW Sample numbers Analyst renewal, or batch used used_termination time 0 1 0I5,O OS-0o-ova q A o\ o cA ,--02.2 -- Oo -c -O O t OOS&50%-0CJ" 3 0, -so -0ý i 1 )St .0V -4 ____-__ 0Do OS -%- OOct o .O-t OOs 5 oL'-Arr) " OS- 2-D9 0o0,o t. o1-'_ Ad E7 c- 101-)Control information: 1 2 Acceptance criteria Summary of test endpoints: % of Male Adults: C ) Q7 *7. s 20% 7-day LC50 ) I ,% Adults having 3 rd Broods: /o067. 1007. >80% NOEC I D07% Mortality:

07. 07.' < 20% LOEC > I067.Mean Offspring/Female:

323.0 ,5!,. t 15.0 offspring/femalc ChV > 1067.% CV: 13%7 L "7 <40.0% IC25 > Io7.T Page 38 of 107

- - Page 2 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant -Non-treated CONTROL -1 Surv Date: Os- 'ival and Reproduction Data Replicate number1 2 3 4 5 6 7 8 9 10--on pr-duced -I Young produced _ 0.0 Adult mortality

_ -._ .I.___ _ .Young produced ' C) o) C- C)Adult mortality ._4 Young produced --A I-A Adult mortality t, L~x _ I_'-I '1-I k1 1 l l°7' [Young produced ____ ____ ____ \_\____x \ .' %t Young produced I_ Ib'A TSIb __ -, t Adult mortality I .IL- VIL I ...- ,_ _Y o un p' ro ducds C,) (D 0- 0 0. 0. 0, 0, 0 Adult mortality (L= e D-= dead)6 Young producedc -S 1 q, Concentration: % Mortality:_Attt\. Mean Offspring/Female: a3.C oNC: 11.3% Survival and Reproduction Data Replicate number Day' 1 2 3- 4 5 6 7 1 8 9 10 TtI Young produced 0 3O 31 0 I A I F Adult mortality L- -- I--t. .I. .L ,__2 ,Young oj l ° °Adult mortality (L live -"= dead)3 YoungCproduced oncet rt * -I on: C 1 I Adult mortality .._.. .4 Young produced Sur l S a nd\eprducionDa _ _na Adult mortality `L- .. q -I .Adult mortality \- \- iv D,=-ad 6 Young produced Q) c 0 c) c0 Lo Qi Concentration: % Mortality: .Mean Offspring/Female: I% Reduction from Control: I -1.17.1.Page 39 of 107 T an l T~ Page 3 of 7 Species: Ceriodaphnia dubia Client: Seauovah Nuclear Plant -Non-treated Date: OS- LI-C)CONC: 22.6%Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced 0) o' 0 0: o c) O Adult mortality % -L I L.__ L_. k 2 Young produced 0l 0 LD c[ o ......Adult mortality II ,- -- ,-I t, Young produced [n 1 I o b a 0 ol 0 Adult mortality I 1 --4 ' I -I -[-V -II 4 Young produced ____ Is __IL ___ 1&_ ___ 'Adult mortality \I-A I,- 4,-- I i.- , Aut mortality I A_____ _[~ ~ I~ [~I~6 Young produced[ C) Cl)l~ o CýI I rAdult mortality I _ ___[l __ __ _--I __ _l-_7 Young produced I t ,\I I'S 19 1 S IS Total young produced l ". I32- Sb- -6.S -6 I6 2"-Final Adult Mortality .- ._ -k. .._Note: Adult mortality (L live, D = dead)Concentration: % Mortality: Mean Offspring/Female: 13g.1% Reduction from Control: " CONe: 45.2% Survival and Reproduction Data Replicate number Day 1i- 2 3 .4 5 6 7 8 9 10 Adult -otlt \_ \- I -,- -I ý- I L-1 I Young produced 0 0. 0 0 0 0 0 0 0 Adult mortality ......_ 1 k ... L L j.23 Young produced CC ICDI-___{ Adult mortality x 1 43 Young produced Adult mortality .Young produced IS___ [-S[ ____6 ___ ___ _Adult mortality -I_ [\- k.-. L... .K I,...5 [Young produced 0_ 0_ C_ 0_ C___f Adult mortality _ _._ _______6 Young produced 1 young produced "5o{ 3A 3 S 3 3,4 "% a, Total youn prdcd=- '~ S ~ 3 q~ I~Final Adult Mortality 1 .. " " _ ._ ._Note: Adult mortality (I. = live, D dead)Concentration: % Mortality: T7 Mean Offspring/Female: n'3 .S% Reduction from Control: -\.S7.Page 40 of 107 l In-, Enlu--u-nalIf -- o tlbs.]Page 4 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant -Non-treated Date: o5-%L-QCk CONC: 72.6%Survival and Reproduction Data Replicate number Day,1 1 2 3 4 5 6 7 8 9 10 I Young produced 0 c 0 _._2 Young produced Q 0 C3 4 3 0 0~~ C_ 100[ Adult mortality L- k.. I .3 You produc 0 0 C 0 0_ o Adult mortality I__ '--I -SYoung produced q I ..Adult mortality j.. ..._I, %._ ._ j 5 Young produced ku [Adult mortality k.-...- _I 1 IL =_=7 , Young produced k[ 's 5 ] ' \. IA 11 I1 \o Total young produced b' Z"' 2 ".Final Adult Mortality i 1 L ' .L '-Note: Adult mortality (L = live, D = dead)Concentration: % Mortality: ONY.Mean Offspring/Female: aq .I% Reduction from Control: -5.37.CONC: 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1I Young produced Q I fi 0 Q 0 o.. i ( 1 Adult mortality U L.- L.. li. L .. L...[Young produced j ) 10c Adult mortality iI °-3 Young produced t I~ ..ci 01 0S 'A 0 kI.Adult mortality _ -1 ... .U._ I..[U 5 Young produced SA I -b 2L Adult mortality -I _.-.1 _. I __'-I '. 1 _ ) I ._I '6 Young produced_1 01 0 01I0 0 lo 5o7o___ Adult mortality j \ý-I ~- ~ I~ _ I I I I .L-7 Young produced ~ I~ j ' ~ ' ~~c ~ I--...-- -j ..-Total young produced Iý , S & z I , 31A 3S 3A .31 Final Adult Mortality L.. T _ .... 1c?_. ...-Note: Adult mortality (L = live, D dead)Concentration: % Mortality: 07.Mean Offspring/Female: 31.i% Reduction from Control: -'-Page 41 of 107 .EnvI-nMWmAlTýtlng Page 5 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant -Non-treated Date: oC- CONTROL-2 Survival and Reproduction Data.... _Replicate number Day , 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 C) 0 0 " 0 C .Adult mortality % -_. .k,.., __. -. L..Adul moraliy i, I- -I\- -- ý ---I-k- 1 3 Young produced 0 C Adult mortality ° I aJ Adult mortality .. .. ._ ._. [ ... _. ..5 Young produced 1 13 =3 )S- 11 1 q -Adult mortality _ _ lLJ ._6 Young produced L n CA C_ __ C_Adult mortality ' °___.__ [.L .13. 1... 13l 1. O 4 4 i.._6 I Young produced \[ I I I-' [7 Young produced 11 o tk -1 1 c I'A 1-1 Total young produced k 3! 3S 3 Final Adult Mortality I v. _ .- X '-.. '... .. I..... -....Note: Adult mortality (L live, D = dead)Concentration: % Mortality: I "L Mean Offsprin Temale: .CONC: 100% Intake Survival and Reproduction Data Replicate number Day,_, 1 2 3 4 5 6 7 8 9 10 2 Young produced G I0.-. 0 I Adult mortality .- __ L .- ___ --'. , '. I ._3 Young produced___ 0 0 j 02- 0f~Iie_Adult mortality F _. ._ ___ L-4 Young produced C) _.. _Adult mortality \_____ ______ ____________.______ ____________ _____6 Young produced SQ 'A qc 1 o- S S I1 0A Adult mortality _5 Young produced -L V\ Iqz~.. 17- 13,. HQ 13 1___ 13 Adult mortality 6 ]Young produced ) 0C o 0 Adult mortality I( 'CI.. .7 Y~oung produced I- 7\ iglo -L_( a is 12 lot, 1--1 Total young produced [ ' 3 __ -" 3o 31 3'Final Adult Mortality %-1... -I ... '.. "l.. .Note:' Adult mortality (L = live, D = dead)Concentration: % Mortality:

)T.Mean Offspring/Female:

1,36.% Reduction from Control: 1 I- i 27.°Page 42 of 107 C 0-1 S 0 0 FTSz Environmenta Testing Solutions, Inc.Control-I TVA / Sequoyah Nuclear Plant, Outfall 101 -Non-treated May 12-19 2009 Verification of Ceriodaphnia Reproduction Totals Day Re licate number --Total y 1 2" 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 5 5 5 5 4 4 45 5 13 13 13 12 13 I1 14 12 12 .12 125 6 0 0 0' 0 0 0 0 0 0 0 0 7 16 17 15 18 16 16 15 17 15 15 160 Total 33 34 33 34 34 32 34 34 31 31 330 11.3%Day Replicate number Total Daa1 2 3 4 5 6 7 8 1 9 10 1 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0. 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 5 5 6 4 4 4 4 4 45 5 12. 14 12 13 13 13 13 14 13 13 130 6 0 0 0 0 0 0 0 0 0 0 0 7 17 17 15 14 17 16 16 16 16 15 159 Total 33 36 32 32 36 33 33 34 33 32 334 22.6%Replicate number Total Day 1 2 3 4 5 6 17 8 9 10-0 0 0 0 0 O1 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0- 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 6 6 4 4 5 4 5 5 48 5. 14 13 13 .14 .11 14 12 13 13 13 130 6 0 0 0 0 0 0 0 0 0 0 0 7 16 19 15 16 17 15 18 17 15 15 163 Total 35 36 34 36 32 33 35 34 33 33 341 45.2%Da Re licate number ay 1 2 3 4 5 6 7 8 9 10 Total 1 0 0 0 0 .0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 5 5 5 5 5 5 4 4 47 5 15 12 12 13 14 14 12" 130 6 0 0 0 0 0 0 0 0 0 0 0 7 16 18 14 17 17 16 15 15 15 15 158 Total 36 34 31 35 35 33 33 34 33 31 335 72.6%Re plicate number Day 1 2 3 1 4 5 6 7 8 1 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 4 5 5 5 4 4 4 4 4 45 5 13 15 15 13 12 13 13 13 14 13 134 6 0 0 0 0 0 0 0 0 0 0 0 7 18 18 16 14 18 15 14 17 16 16 162 Total 37 37 36 32 35 32 31 34 34 33 .341 100%Day Replicate number Total 1 2 3 4 5 6 7 8 "9"T 10 1 0 0 0 0 0a 0 0 0 a 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 6 4 5 5 5 4 6 6 4 50 5 15 14 12 14 13 14 13 13 13 12 133 6 0 0 0 0 0 0 0 0 0 0 0 7 19 18 16 17 16 14 17 16 15 15 163 Total 39 38 32 36 34 33 34 35 34 31 346 Control-2 Day Re plicate number Total 1 2 3 ,4 5 6 7 8 91" 10 1 0 -0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 -0 0 0 0 0 0 4 4 4 4 4 5 4 5 4 4 4 42 5 14 12 13 13 13 13 12 10 14 12 126 6 0 0 0 0 0 0 0 0 0 0 0 7 15 19 16 18 16 16 14 17 15 17 163 Total 33 35 33 35 34 33 31 31 33 33 331 100% Intake Day Re licate number Total 1 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 6 4 4 5 5 5 5 4 47 5 15 12 14 14 12 13 14 13 13 13 133 6 0 0 0 0 0 0 0 0 0 0 0 7 19 19 21 18 19 20 18 18 19 17 188 Total 39 35 41 36 35 38 37 36 37 34 368 -v Environmental.Testing Solutions, Inc.TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 12-19, 2009 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: Reveiwed by: 5444 Concentration Replicate number Survival Average reproduction Coeffcien(of Percent reduction from (%) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) va"at"on (%) pooled controls (%)Control- 1 33 34 33 34 34 32 34 34 31 31 100 33.0 3.8 Not applicable 11.3% 33 36 32 32 36 33 33 34 33 32 100 33.4 4.5 -1.2 22.6% 35 36 34 36 32 33 35 34 33 33 100 34.1 4.0 -3.3 45.2%/o 36 34 31 35 35 33 33 34 33 31 100 33.5 4.9 -1.5 72.6% 37 37 36 32 35 32 31 34 34 33 100 34.1 6.3 -3.3 100% 39 38 32 36 34 33 34 35 34 31 100 34.6 7.2 -4.8 Control -2 33 35 33 35 34 33 31 31 33 33 100 33.1 4.1 Not applicable 100% Intake 39 35 41 36 35 38 37 36 37 34 100 36;8 5.7 -11.2 Outfall 101: Dunnett's MSD value: PMSD: Intakce Dunnett's MSD value: PMSD: MSD =PMSD =1.831 5.5 1.374 4.2 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.Lower PMSD bound determined by USEPA (IOt percentile) = 13%.Upper PMSD bound determined.by USEPA (90' percentile) = 47%.Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001 b), USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix_ EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. (11 0q--..1 o'.) Environmental Testing S olutions0 inc.TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 12-19, 2009 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:. 5/12/2009 Test ID: CdFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: :5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-82 I-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: -Conc-% 1 2 3 4 5 6 7 8 9 " 10 Control- 1 33.000 34.000 33.000 .34.000 .34.000 32.000 34.000 34.000 31.000 31.000 Control-2 33.000 35.000 33.000 35.000 34.000 33.000 31.000 31.000 33.000 33.000 11.3 33.000 36.000 32.000 32.000 36.000 33.000 33.000 34.000 33.000 32.000 22.6 35.000 36.000 34.000 36.000 32.000 33.000 35.000 34.000 33.000 33.000 45.2 36.000 34.000 31.000 35.000 35.000 33.000 33.000 34.000 33.000 31.000 72.6 37.000 37.000 36.000 32.000 35.000 32.000 31.000 34.000 34.000 33.000 100 39.000 38.000 32.000 36.000 34.000 33.000 34.000 35.000 34.000 31.000 Intake 39.000 35.000 41.000 36.000 35.000 38.000 37.000 36.000 37.000 34.000 Transform: Untransformed I -Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean Control-I 33.000 0.9970 33.000 31.000 34.000 3.779 10 33.783 1.0000 Control-2 33.100 1.0000 33.100 31.000 35.000 .4.140 10 11.3 33.400 1.0091 33.400 32.000 36.000 4.508 10 -0.500 2.287 1.831 33.783 1.0000 22.6 34.100 1.0302 34.100 32.000 36.000 4.019 10 -1.374 2.287 1.831 33.783 1.0000 45.2 33.500 1.0121 33.500 -31.000 36.000 4.925 10 -0.624 2.287 1.831 33.783 1.0000 72.6 34.100 1.0302 34.100 31.000 37.000 6.252 10 -1.374 2.287 1.831 33.783 1.0000 100 34.600 1.0453 34.600 31.000 39.000 7.235 10 -1.998 2.287 1.831 33.783 1.0000 Intake 36.800 1.1118 36.800 34.000 41.000 5.700 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.653808415 1.035 0.254140335 -0.23607601 Bartlett's Test indicates equal variances (p = 0.26) 6.457315922 15.08627224 The control means are not significantly different (p = 0.87) 0.170664037 2.100922029 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV .TU .MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 1.83092058 0.055482442 3.416666667 3.205555556 0.389506936 5,54 Treatments vs Control-I Linear Interpolation (200 Resamples) Point % SD 95% CL Skew IC05 ICI0 ICI5 IC20 IC25 IC40 IC50>100>100>100>100>100>100>100 sqnlOlO5-12-O9data 0-1 TVA / Sequoyah Nuclear Plant, Outfall 101 -Intake Non-treated May 12-19, 2009EnvironmentalTesting Solutions. Inc.Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/12/2009 Test ID: CdFRCR Sample ID: TVA / Sequoyah Nuclear Plant, OutfaU 101 -Intake End Date: 5/19/2009 LUb ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-% 1 2 3 4 5 6 7 8 9 -10 Control-I 33.000 34.000 33.000 34.000 34.000 32.000 34.000 34.000 31.000 31.000 Control-2 33.000 35.000 33.000 35.000 34.000 ' 33.000 31.000 31.000 33.000 33.000 11.3 33.000 36.000 32.000 32.000 36.000 33.000 33.000 34.000 33.000 32.000 22.6 35.000 36.000 34.000 36.000, 32.000 33.000 35.000 34.000 33.000 33.000ý45.2 36.000 34.000 31.000 35.000 35.000 33.000 33.000 34.000 33.000 31.000 72.6 37.000 37.000 36.000 32.000 35.000 32.000 31.000 34.000 34.000 33.000 100 39.000 38.000 32.000 36.000 34.000 33.000 34.000 35.000 34.000 31.000 Intake 39.000 35.000 41.000 36.000 35.000 38.000 37.000 36.000 37.000 34.000 Transform: Untransformed I-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-I 33.000 0.9970 33.000 31.000 34.000 3.779 10 Control-2 33.100 1.0000 33.100 31.000 35.000 4.140 10 11.3 331400 1,0091 33.400 32.000 36.000 4.508 10 22.6 34.100 1.0302 34.100 32.000 36.000 4.019 10 45.2 33.500 1.0121 33.500 31.000 36.000 4.925 10 72.6 34.100 1.0302 34.100 31.000 37.000 6.252 10 100 341600 1.0453 34.600 31.000 39.000 7.235 10 Intake 36.800 1.1118 36.800 34.000 41.000 5.700 10 -4.670 1.734 1.374 Auxiliary Tests Statistic* Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0H01) 0.955419481 0.868 0.544660572 0.427540628 F-Test indicates equal variances (Ii = 0.22) 2.3431952 6.541089535 The control means are not significantly different (p = 0.87) 0.170664037 2.100922029 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.373940728 0.041508783 68.45 3.138888889 1.9E-04 I, 18 Treatments vs Control-2 sqn lOl_O5-12-O9data TVA / Sequoyah Nuclear Plant, Outfall 101 -Non-treated May 12-19, 2009 Environmental Testing Solutions, Inc.Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Project number; 5444 Reviewed by: Concentration Parameter Da 0 Da 1 Day 2 DaD Da 4 Da 5 Da_________ ________________ Initial Final Initial IFinal Initial Final Initial .Fnl Initial IFinal IInitial IFinal Initial Fia PH (SU 7.32 7.41 7.601 7.431 7.6 '5 7.21 7.58 7.1 7.601 7.471 7.431 7.40 7.66 73 DO (mgIL) 7-5 7.6 7.61 7.8 7.4 7.8 7.7 777.81 7.51 7.61 7.6 7.47.Conductivity (gmhos/cm) 3l7 302 326 3383 328 Control Alkalinity (mg/L CaCO 3) 61 61 Hardness (mg/L CaCO 3) 92 88 rTemperature ('C) 25.0 25.2 25.0 24. 24.7 24.8 24.7 24.8 24.7 25.0 24.8 8 73 722 7.44.4 pH (SU.) 7.47 7.4] 7.40 7.43 7.39 7.2 7.26 7.4 7.41 7.49 7.50 7.40 7.42 70 DO (mg/L) 7.5 7.5 7.6 7.7 7.6 7.8 8.0 7.5 7.7 7.6 7.5 4 11.3% Conductivity (Ismhos/cm) 293 292 284 298 304 300 Temperature (C) 25.0 25.2 25.0 24.9 24.7 24.8 25.0 24.9 24.8 25.0 24.7 25.0 24.8 0 H S).7.47 7.40 7.41 7.42 7.37 7.22 7.26 7.43 7.40 7.48 .7.49 7.0 7.41 73 2.% DO (m L). 7.5 7.6 7.71 7.7, 7.6 7.6 7.8 7.7 8.1 7.6 T7.76 7.51 7.4 22.6%Conductivity (Amhos/cm) 284 277 268 279 28 280 287_Temperature (C) 25.0 25.0 25.1 24.9 24:7 24.8 25.0 25.2 24.8 24.8 24.7 24.8 pH (SUJ) 7.47 7.39 741 .421 7.31 7.22 7.23 7.42 7.36 7.48 7.45 7.387.3 3 45 0 DO (minL) 7.7 7.6 7.7 7.61 7.6 7.6 7.8 7.7 8.0767 7.6 7.6 746 Conductivity (jsmhos/cm) 247 243 234 243 247 243 46 rTemperature (C) 249 252 251 249 248 249 25.0 252 249 248 248 24.9 PH (SU) .7.46 7.38 7.35 7.37 7.27 7.22 7.18 7.38 7.30 7.42 7.42 7.57.32 7.32 DO (mg/L) .7 76 77 77 77 77 7.9 76 78 76 77 7.8 Conductivity (iimhos/cm) 205 202 189 19 196 196 199 Temperature ('C) 24.9 25.1 25.1 24.9 24.8 24.7 25.0 25.2 24.9 249 248 24.9 24.9 24.9 pH (SU) 7.39 7.34 730 733 7.19 7.20 .14 736 7.24 738 36 7.30 7.26 7.28 DO (mgL) 7.7 7.6 77 77 7.7 7.7 7.8 7.6 7.8 76 77 7.6 7.8 7.5 Conductivity (pmhos/cm) .5 154 145 152 1461 100% Alkalinity (mg/L CaCO 3) 65 57 50 Hardness (mg/L CaCO 3) .62 60 Total Residual Chlorine (mL) <O. 1 0.10_______Temperature ('C) .24.9 25.1 25.11 24.8 24.81 24.7 25.01 25..0 25.01 24.81 24.81 24.91 24.91 24.91 pH (SU) 7.39 7.32 7.30 7.30 7.171 7.09 7.11 7.33 7.22 735 7.34 7.30 7.25 7.26 DO (mgIL) 7.8 7.6 7.71 7.6 .7.81 7.71 7..9 7.6 7.81 .7.51 7.71 7.5 7.8 7.5 Conductivi mhs/cm 153 152 143 18 14514 100%-Intake Alkalinity (mg/L CaCO 3) 57 54 52 Hardness (mg/L CaCO 3) 64 64 60 Total Residual Chlorine (<0. 10 ý <0,10 <0.10_______Temperature (*C) 24.91 25.11 25.11 24.8 24.81 24.61 .2. 25.0 24.81 24.91 24.81 24.91 24.9 24..9

- - Page 6 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant -Non-treated Daily Chemistry:

Date: 0c- i'-o4\Day 0 ~ 12 Analyst ll A A e ^-L1 1-\LL Concentration Parameter CONTROL pH (S.U.) -4.32 DO (mg/L) 1,-, T" .L4f--.Conductivity 3 1 3tQ (iimhos/cm)37 Alkalinity (mg CaCO3IL)Hardness (mg CaCO3/L) CA-Temperature (C) vs.O LS"L. t .O ., ..".PH (S.U.) 7H4 17 0 ý11.3% DO (mg/L) ~7-Conductivity ...Temperature(°C) ( .'Q O LS.O 7M-0 7. .1 pH (S.U.)3 II ;4A-; 1 I 4-,L II T1- a I :li3 H 1.22 1 22.6%DO (mg/L)., Conductivity (11hos/cm) ]T S, -- -L *I ". !Li of, , Iuil I[ *, b em era re I" 45.2%DO (mg/L) 'Conductivity (ýimhos/cm) 10A -R JL 1q 4 Temperature (TC)!PH(S.U.) 3 t .I i3 I. i -72.6% ~jDO (mg/L) Ii~J- 17L7L Conductivity (Timhospctu) I Temperatur (TC) -Ls 1 .'.,q9 -pH (S.U.)3.33 I II --4. 1 II ,1-.zo I 100%DO (mg/L)Conductivity (pimhos/cm) Alkalinity (mg CaCO 3/L)Hardness (mg CaCO 3/L)TR chlorine (mg/L) I<-4 1- , I ... "4- ?0.11)7'1. A I 1-1 1S. 1 .. i q. -"4.7 Temperature (QC)* p -pH (S.U.)I 3.3; ) I13o I I1 II '} ,1 I I ~ --100% Intake DO (mg/L)1I 1 Z Conductivity Alkalinity (mg CaCO 3 IL) 1 Hardness (mg CaCO 3/L) (A TR chlorine (mg/L) .0.10.r8 S0.\i I e. ..t Temperature (TC)7-4.H a -a II

II *Initial Final Initial Final Initial I Final " Page 48 of 107
- .-E .-mmha elngS~lmIc Page 7 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant -Non-treated
Date: 05- %"L-cf-U I I Day I1 I I 3 I 4 I 5 I 6 II S -Analyst I Concen- I Parameter tration CONTROL pH (S.U.)/DO (mg/L)Conductivity I (Aimhos/cm)

Alkalinity (mg CaCO/L)HardnessI (Mg CaCO 3/L,)~1. P 32_1o 33F3 Pei0 Temnerature (ofl"9 pH(S.U.) 1,2cp ".4 .7 7.Ac .1. b (t',p .11.3% DO (mg/L) M Conductivity 30q_____ gLS. -LA 1. 1 .0 bV vb/Conductivity ____Temperature (0 CQ IA. -AA~x .~4 ~pH,(S.U.) (_V 141q . 25.2% DO mg/ll, Conductivity (pmhosicm) (4 Temperature(°C) iQL. o -.'- 1 I , * / I I L *" PH (S.U.) 7 4 7 72.6% 'DO*mg/fl_ Conductivity (prnhos/cm) II7 Temperature (°C) 05.0 2s".';.. 7A.- .L I.qk " .PH p.¢U.) 4,1 -1 7200% DO (mg/L)9q 100% DO m.) Conductivity mhIos/ m) WI PH_(.U.co) q.O Hardness .00 (rag CaCO3./L)TR Chlorine (mg/L) <0.10 Temperature (C) .0 I ..1U .VA .-.1 l PH (s.U.) I5)I73 -, -.100% DO(gL Pmhos/cm) '+4 .Alkalinity "-2 l (mag CaCO3/L)S "Hardness TR chlorine (mg/L) < 0. 1c)Temperatur (T -.o -q e 11 z. , zl I Initial 1 Final I Initial 11 Final I Initial 11 Final I Initial I Final ,1 Page 49 of 107 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)Species: Pimeph ales promelas Page 1 of 6 Client: TVA Facility: Sequoyah Nuclear Plant NPDES #: TN 0026450 Project #: AALk County: Hamilton Treatment: UV-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Each concentration was treated Effluent volume (mL) 282.5 565 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2217.5 1935 1370 685 0 to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences. Test organism information: Test information: Organism age: 7o.Ss Aooc, 1j Randomizing template: Date and times organisms o -\ o Incubator number and were born between: __shelf location: Organism source: týr &'iUA Pp M -I \-o1 Artemia CHM number: L,.LA44 Transfer bowl information: pH = SU Temperature = 'C Date / Time in: 05. A-ON %oo" Date / Time out: OS Zo-Cn It.2O Average transfer volume: Total drying time: tq. -. U t$,vt u. "Ths Initial oven temperature: ko(" ^ U Final oven temperature: oV U Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, renewal, Sample MHSW or termination numbers used batch used Time Analyst Time Analyst Time Analyst 0 o'Z,- -it-cft _____ 1-Do3 .VZ:5L.. WCfN o 4-LO oS-d9-OOA 1---_ 140__ _ _ _ _ _ _t) I__-____ c c -o , os.-t'-dc S odocl A, ,I o

o--3__ OS.d I'S. cr 1 Ld 00 A22 I t~~ 2C)5 *O'1 OS-DO'-11, 4 cfs OýPoo 4L Iqc 2A Qc a G~l-r,4 OI US- 12-cPR 6 Gs -is- 0 0 1o oA3,4:-lq3-1 6qO51r, -O-Z 4-ni n<-11-cft 7 -f0- Oc figo _ _ _ _ _ _r_'Control information:

Acceptance criteria Summary of test endpoints: % Mortality: < 20% 7-day LC 5 0 > It 07.Average weight per initial larvae: 0. 6 &- NOEC o00"7..Average weight per surviving larvae: O. (0 VL4 _ 0.25 mg/larvae LOEC I 14 ChV IC2s .>tool.Page 50 of 107 Page 2 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant -UV-treated Date: OC-v2--ol Survival and Growth Data Day CONTROL 11.3% 22.6%A B C D E F G H I J K L 0 I 10 10 /t to //0 0 '0 /0 /0 10 /0 2 10 O to t0 It o0 /0 /o t / /0 //3 /1 C 0 to /0 /0 ip ./ /0 /o C0 /0 t0 50 Io 0t /0 (0 /0 !0 /0 /0 (0 '0 /0 /Q0 650t D t D I t iI O / C/0 1 D I0 to to t' toO I0 IO 10 c ,/6 I0 10 ( ) 0 1Q / /D to (0, 10 ' / 10 10, 10 10 t0 (0 'o (0t (0 j0 to to A = Pan weight (mg)Tray color olf:: lt 13.8o i~lyý )4.60 ý-60 /o Itt 13.-9, tK ILL 14 ILL 1 Analyst: Date: p0j 9 -- 0 _B = Pan + Larvae weight (mg) P ..3 -1.o1q -.35 , .Ib a.I.h'L, U JA-3 .l.'b ).0i, Analyst: \Date: 0 5" -'&,0 -00, C = Larvae weight (mg)=B-A.-1s .4s 0,,,6. (#.sot -p 3a S I , 1-56 1. 11 a -rz ..b ..' Weight per initial number of larvae (mg)= C / Initial number of larvae'a C, CA.to, N'V SID&I'.A*4.0*Average Percent weight per reduction initial from control 0. IOR lranumber of (%A ~a f a a aba Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.Calculations and data reviewed: _4&Comments: Page 51 of 107 F T 1nm Page 3 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant -UV-treated Date: OS -1L--CYN Survival and Growth Data Day 45.2% 72.6% 100%M N 0 P Q R S T U V W X 0 0 M 10 /D 1 /0 a /I b /0 /t0 tO 0 1 /0 10 /D /0 /0 /1 /0 /0 /0 /0 /0 /0 2 C0 / IC ( /0/0 10 0 to /0 /0 /0 /0 31 .0 I0 ID 1 0 t0 10 to /0 I/0 10 4 I QO t 0_/0 ID 100 /0 '0 10 /0 /0 / 0 /0 /Q.5 to 10 o 1 ( /0 /0 to /0 /0 0 10 /0 6 I (Q 10 I. (0 I 10 C IL) K I/ lk '0 /0 10.70 ID 1- I( 10 10 /0 /0 /0 IO io It)A Pan weight (mg)Tray color code:: IN.Iq" I.oa 13.q " t. Jq.sT t '3 1. toL.- I.. Ii" I?.jI Analyst: r-L, 5-r1q 1o 31ý 1-8 J-S l~ l 1-q ). 0 ), 1.I 34 Date: 6 ""I. B = Pan + Larvae weight (rag) [.I! IT 1,qa, P.1-D)l p.s"o .l o l p)0, /q~ql ;.lot 4 g 4o o. Vq Analyst: ALsr" _ r Date: Q T"-0 -0 q_C = Larvae weight (mg)=B-A Weight per initial number of larvae (mg) ' pv=C / Initial number of larvae I., " o" 0" 0" 3" " O O'Average Percent.weight per reduction initial from control Z .LA A 0.1., --'I A V 2..7T number of (%)larvae (mgB)Comment codes: c clear, d dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.Calculations and data reviewed: Comments: Page 52 of 107 Comments:

)ETS Page 4 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant -UV-treated Date: O'S- 7 -Oq Survival and Growth Data Day 100% Intake Y Z AA BB 0 2/0 /0 /0 /0 t o me 4 .5 to /0 /0 6 IQL 0 (10 to to to A = Pan weight (mg)Tray color code:: 1ý t.1 jl.Ri 1 1',D i1.,qL1 Analyst: r"Lr Date: "t-O -O B = Pan + Larvae weight.(rg) -.I ý.lql'U- g.a Iq3js Analyst: nL f", Date: 99- 3.0- Ott C = Larvae weight (mg)=B-A Weight per initial number of larvae (rmg)= C /Initial number of larvae \,a 'a Average Percent weight per reduction initial from control number of (%)larvae (mg)Comment codes: c = clear, d = dead, fg fungus, k = killed, m missing, sk sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.Calculations and data reviewed: Comments: Page 53 of 107

'. Environmental Testing Solutions, Inc.TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 12-19, 2009 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 5444 Reveiwed by: /'-(i YiA[l---Not for Compliance Assessment Internal Laboratory QC Cusaeetratioa (% Rqplicate

aItala .mbeod Find uu.bera of A P -right B=an +La-,.'a L.ac oight(.g)

WWigktJS-riviMg M. dght I C/ adf leta-riatio Wdgka Ilid it aber Meau-uvhu.l M-a eight It CoCeffliPaerl Pent rcmtnuc ioafrou tnrva -Lrvac (-9 wright (rg) -A-B .mbnruof irvae(maW S. rvguiA.mbrrof -1 , ofalarvua(mg) (%) lIlallunmberof vrarido (m.-O, coatrol (%)-larvae (ug)

urvae (erg) ~Me....A I0 10 13 85' 20.22 6.37 0.637 0.637 " Control B 10 10 13.62 21.05 7.43 0.743 0.684 6.8 0.743 100.0 0.644 6.8 Notappleable C 10 o0 13.80 20.75 6.95 0.695 0.695 D 10 10 14.45 21.04 6.59 0.659 0.659 E 10 10 14.60 22.35 7.75 0.775 0.775 F 10 10 13.77 22.29 8.52 0.852 0789 5.4 0.852 G 10 10 12.60 20.16 7.56 .0.756 0 0.756 100.0 0.789 5.4 -15.4 H 10 0 14.1 4 21.86 7.72 0.772 0.772 1 10 10 13.86 21.23 7.37 0.737 0.737 22.6% .10 10 14 15 21.42 7.27 0,727 0.727 K 10 10 14.14 21.20 7.06 .0.706 0.724 1.8 0.706 975 0.706 5.3 3.3 L 10 9 14.31 20.84 6.53 0.726 0.653 M 10 10 15.59 .2252 6.93 0.693 0.693 45.2% N o 010 13.95 21.17 7.22 0,722 0.114 4.6 0.722 100.0 0.714 4.6 -4.4 0 10 10 13.09 19.92 6.83 0.683 0.683.P to 10 13.45 21.01 7.56 0.756 0.756 10 10 13.68 20.50 6.82 0.682 0.682 72.6% R 10 10 14.35 21.03
6.68 0.668 0.684 3.8 0.668. 100.0 0.684 3.8 -0.1 S 10 10 14.41 21.06 6.65 0.665 0.665 1 T 10 10 13.34 20.56 7.22 0.722 0.722 u U 10 10 13.60 19 91 6.31 0.631 0.631 V IV 00 1440 21.04 6.64 0.664 0698 8.5 0.664 W 10 10 14.19 21-.70 7.51 0.751 0.751 X [0 10 13,44 20.89 7.45 0.745 1 0.745 Y 10 10 -14.20 21.15 6.95 0.695 0.695 100% Intake Z 0 10 1482 21 17 6.35 0.635 0.659 4.1 0.635 AA i 0 1o 14,20 20.82 6.62 0.662 0.662 100.0 0.659 4.1 37 1B 10 1 0 12.96 19,38 6.42 0.642 0.642 Outfall 101: Durnett's MSD value: PMSD: Intake: Dunnelt's MSD value: PMSD: 0.0720 10.5 0.0520 7.6 MSD = Minimum Significant Difference.

PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.Lower PMSD bound determined by. USEPA (lOth percentile) = 12%.Upper PMSD bound determined by USEPA (90th percentile) 30%.Lower and upper PMSD bounds were determined from the loth and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2 0 0 1a; USEPA, 2001b)USEPA. 2001 a, 2001b. Final Report: interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B.-01--04 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. (ti"-a TEnvironmental TestIng Solutions, Inc.TVA / Sequoyah Nuclear Plant, Outfall 101 U V-treated May 12-19, 2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample lD: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% Y- 2 3 4 D-Control 0.6370 0.7430 0.6950 0.6590 11.3 0.7750 0.8520 0.7560 0-7720 22.6 0.7370 0.7270 0.7060 0.6530 45.2 0.6930 0,7220 0.6830 0.7560 72.6 0.6820 06680 0.6650 .0.7220 100 0.6310 0,6640 0.7510 0.7450 Intake 0.6950 0.6350 0.6620 0.6420 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6835 1.0000 0.6835 0.6370 0.7430 6.776 4 0.7361 1.0000 11.3 0.7888 1.1540 0.7888 0.7560 0.8520 5.450 4 -3.522 2.410 0.0720 0.7361 1.0000 22.6 0.7058 1.0326 0.7058 0.6530 0.7370 5.308 4 -0.745 2.410 0.0720 0.7096 0.9640 45.2 0.7135 1.0439 0.7135 0.6830 0.7560 4.598 4 -1.004 2.410 0.0720 0.7096 0.9640 72.6 0.6843 1.0011 0.6843 0.6650 0.7220 3.834 4 -0.025 2.410 0.0720 0.6910 0.9387 100 0.6978 1.0208 0.6978 0.6310 0.7510 8.544 4 -0.477 2.410 0.0720 0.6910 0.9387 Intake 0.6585 0.9634 0.6585 0.6350 0.6950 4.083 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.957272053 0.884 0.185406404 -0.99302071 Bartlett's Test indicates equal variances (p 0.83) 2.111745119 15.08627224 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.072014927 0.105361999 0.0061735 0.001785833 0.023020582 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point % S D 95% CL(Exp) Skew IC05 ICI0 IC15 1C20 IC25 IC40 IC50 60.362>100>100>100>100>100>100 sqn101_05-12-O9data-uv -.Z U14 TVA Sequoyah Nuclear Plant, Outfall 101 -Intake UV-treated May 12-19, 2009 0 0.Environmental Testing Solutions, Inc.Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 -Intake End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% 1 2 3 4 D-Control 0.6370 0.7430 0.6950 0.6590 11.3 0.7750 0.8520 0.7560 0.7720 22.6 0.7370 0.7270 0.7060 0.6530 45.2 0.6930 0.7220 0.6830 0.7560 72.6 0.6820 0.6680 0.6650 0.7220 100 0.6310 0.6640 0.7510 0.7450 Intake 0.6950 0.6350 0.6620 0.6420 Transform: Untransformed 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.6835 1.0000 0.6835 0.6370 0.7430 6.776 4 11.3 0.7888 1.1540 0.7888 0.7560 0.8520 5.450 4 22.6 0.7058 1.0326 0.7058 0.6530 0.7370 5.308 4 45.2 0.7135 1.0439 0.7135 0.6830 0.7560 4.598 4 72.6 0.6843 1.0011 0.6843 0.6650 0.7220 3.834 4 100 0.6978 1.0208 0.6978 0.6310 0.7510 8.544 4 Intake 0.6585 0.9634 0.6585 0.6350 0.6950 4.083 4 0.934 1.943 0.0520 Auxiliary Tests .Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.0 1) 0.95557791 0.749 0.566287708 -0.42008898 F-Test indicates equal variances (p = 0.40) 2.96680498 47.46722794 Hypothesis Test (I-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic I Test indicates no significant differences 0.05203226 0.0761262 0.00125 0.001434 0.386520296 1,6 Treatments vs D-Control sqnl O_015-12-09data-uv --Environmental Testing Solutions, Inc.TVA / Sequoyah Nuclear Plant, Outfall 101 -UV-treated May 12-19, 2009 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number: 5444 Reviewedby Concentration Parameter Da 0 Da I Da Da 3 Da 4 1 D 5 Day 6 Initial Final Initial IFinal Initial Fni Initial Final Initial IFinal IInitial Fnl Initial Fia pH (SU) 7.51 7.26 7.42 7.2 7.15 7.28 7.38 7.49 7.44 7.53 7.42 7.50 7.04 DO (mg/L) 7.8 7.7 7.6 7.5 7.5 7.8 7.6 7.81 7.61 7 7 Conductivity (limhos/cm) 313 296 317 3 Control Alkalinity (mg/L CaCO 3) 61 59 Hardness (mg/L CaCO 3) 88 92 Temperature (OC) 24.8 24.8 24.8 24.9 .824.8 24.6 24.8 24.6 24.8 24.6 24.7 24.5 24.9 24.41 pH (SU) 7.52 7.20 7.43 7.24 7.40 7.15 7.29 7.34 7.49 7.38 7.53 7.38 7.52 7.081.DO (mg/L) 7.9 7.2 .7.7 7.3 7.5 7.5 7.8 7.3 7.7 .7.3 7.7 7.5 7.7 6.8 Conductivity (plmhos/cm) 301 29 282 302 303 321...... _Temperature ('C) 24.8 24.7 25.0 24.8 24.7 24.4 24.8 24.6 24.84 24.6 24.7 24.5 24.9 24.4 pH (SU) 7.52 7.19 7.43 7.24 7.39 7.11 7.29 7.28 7.48 7.394 7.54 7.371 7.50 7.02% DO (mL) 7,9 7.3 7.8 7.2 7.5 7.5 7.9 7.2 7.7 7.4 7.7 7.4 .7 6.8 22.6% Conductivity (pmhos/cm) 284 28. 280J 264 281 28 283 299_ Temperature ('C) 24.8 24.7 24.9 24.5 24.7 24.4 24.7 24.5 24.8 24.4 24.7 24.6 24.9 24.4 PH (SU) 7.51 7.15 7.42 7.18 7.34 7.11 7.28 7.30 7.44 7.38 7.50 7.32 7.48 7.03 DO(mg/L 7.9 7.2 7.8, 7.1 7.6 7.4 8.0 7.4 7.7 7.4 7.8 7.4 7.7 6.8 4.% Conductivity (psmbos/cm) .247 244 ý 231 245 246 ý N 248 ý 258 Temperature (°C) "24.8 24.9 24.9 24.4 24.7 24.3 24.7 24.5 24.9 24.3 24.8 24.6 24.9 24.5 pH (SU) 7.47 7.19 7.38 7.13 7.29 7.12 7.23 7.27 7.39 7.30 7.49 7.36 7.43 7.03 DO(mg/L) 8.0 7.1 7.8 7.1 7.6 7.4 8.0 7.3 7.7 7.4 7.8 7.5 7.6 6.8 72. 6% Conductivity (ýunhos/cm), 204 200 189 206 198 197 2 Temperature (TC) 24.8 24.81 24.94 24.6 24.7 24.5 24.7 24.3 24.9 24.7 24.9 246 24. 245N pH (SU) 7431 7.191 7.34 7.14 7.25 7.11 7.19 7.301 7.34 7.29 741 7.28 7381.95 DO (mgL) 7.91 7.11 7.81 7.1, 7.8 7.4 8.01 7.41 7.7, 7.3 7.8 751 7.61 6 .8 Conductivi mhos/cm 159115 148 157 157 100% Alkalinity. (mg/L CaCO 3) 52 444 Hardness (mg/I CaCO 3) -62 64 Temperature ('C) 24.81 24.81 24.9 24.6 24.8 24.5 24.71 24.7 25.01 24.31 24.9 24.4 24.8 24.6 pH (SU) 7.43 7.111 7.31 7.13 7.19 7.01 7.15 7.2 7.311 7.29 7.38 7.24 7.361 6.89 DO (m 7.9 7.2 7.8 7.1 7.8 7.4 7.9 7. 7.8 7.3 7.71 7.5 7.6 68 Conductivity(Imhos/cm). 154 141146 100% Intake Alkalinity (mg/L CaCO 3) 57 5250 Hardness (mg/L CaCO 3) 66 62 8 Temperature ('C) 24.8 25.0 24.8 24.7 25.0 24.6 24.75 1.5 24.51 4 24.4 24.91 24.4

DT Page 5 of 6 Species: Pbmephales promelas Client: TVA / Sequoyah Nuclear Plant -UV-treated Daily Chemistry:

Date: Os-1"_- 20'Day Concentration Parameter CONTROL pH (S.U.)DO (mg/L) ."r ' : -Conductivity " 13 (ý+/-mO*M) ýi Alkalinity (mg CaCO 3/L)Hardness (mg CaCO 3/L).__________ Temperature ( ."Q. -"AAk ".. L .9 '" A., pH (S.U.) .44 14 11.3% DO (mgb 1-)7 -4S Conductivity 7ý&(pnhos/cm) Temperature (0 Q 1A.k -LtS. -V. 0 PH (S.U.) --7 1-4-43 -1,~ T3q }22.6% -DO (mg °b ). .,.9 1-5 Conductivity _________ Temperature QC) .4~iA AS t DR (S.U.)"-; -II "7-s I T-,q3 U J- I 1 ".1-3 IUF.11!I r % .... ] I 45.2%DO (mg/b)Conductivity (Ltmhos/cm) 7 ,9 1 1 4-1 I ."1 -4,q.ý3L11(I____?_Hqa_3__-n Temlerature (C)i_______________ -& .-.' --- ? I Tq pH (S.U.),L 4 1-1 --q 3 ' 9.] 1I f-13 I d.,q I 31,I 72.6%DO (mg/L)Ell r. I Conductivity l lAlc~.d I Temperature ( iC)-IA- F< I 2A.9& .& ~* II

1 II I1 pH (S.U.)-4.L/-? 1 1 .3-4 II i-ý L4 ý -I a 5 .1 100%DO (mg/L)Conductivity (gmhos/cm)

_____Alkalinity (mg CaCOt3L) ."_..Hardness (mg CaCO3/1__TR chlorine (mg/L)Temperature (°C)2 II 1 PH (S.U.) "? 3 1 100% Intake DO (mg/b)Conductivity

" ..Alkalinity (mg CaCO /L)62 Hardness (mg CaCO 3/L) 67-TR chlorine (mg/L) < 0. '0-0_ Temperature

(°C) ? 94 j -". -I C8 , Initial 11 Final I. Initial I Final I Initial I Final Page 58 of 107 bT.Page 6 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant -UV-treated Date: 7 u -112.-01)0\e' op U I Day, N I p I 0-3 I 4 I \5 I 6 I Analyst Concen- Parameter tration CONTROL DH (S.U.)DO (mg/L)Conductivity (u.mhos/cm) Alkalinity (rMg CaCO 3/L) 41 .-TO"-.A. -. So l. iA. n .S --. .Hardness (mr CaCO/L)Temperature (MC)S " " " I pH (S.U.)11.3%DO (mg/L)Conductivity (iimhoslcm) -1.3! 1-1.7T&2t2. 303 1A m~~2A 1 I Temperature (MC)pH (S.U.)22.6% DO (mg/L) 4 Conductivity (ýimhos/cm) 2E ý3______Temperature ('C) 2'-. f~S1'~ 1+-1 1.pH-(S.U.)

1. 0 .49 -34.7 , 45.2% DO_(mg8L0

.-7 -7 .Conductivity -(p~mhos/cm) Temperature(0 C) 1 -A.7 '--" "., 7.pH (S.U.) __7___3 72.6% DO (mg/L) .7 -1 Conductivity (gmhos/cm) ______Temnperature ('Q) Iq~ "t~1L~~-A1 A74~ 4 100%. DO (mg/L)Alkalinity m (mg CaCOJL1) Hardness (mag CaCO1/L) WD0 TR Chlorine (mg/L) < 6, ID__ __ __ Tern erature ('C ).1 m+ .... .---.[ a " pH (S.U.)I Q 16 -7 *.v7 I 7.371 II ', I -*1.2 1' m2 "L.' I -4. 1 AI ,q I 100%Intake DO (mg/L)I -I-'. (4 Conductivity pmhos/cm)Alkalinity (mg CaC h/L)Hardness TR chlorine (mg/L)Temperature (QC)SO 1;o. ,0 l a I""'l I ý-' I is."0 I 'j 1 I 1'7 1 7.4'-- I -'.Initial Final I Initial Final I Initial H Final I Initial 11 Final I Page 59 of 107 Page ot,1 Page I of I ng ETS Environmental TestingSouinm -56's%Total Residual Chlorine (Orion Electrode Method, Orion 97-70)Matrix: Water,'RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst F7% L7 77 Date analyzed 5 -)a. 6 ]Iodide reagent: 1AR 334 Acid reagent: In 3-3ý d Calibration: 0.10 mIL 1.00m I Reference standard number I XA~s ý13 I Ai 7 Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV /TV x 100 number (mg/L) (mg/L) (acceptable range =90 to 110%)I 3%s 67- 3 0.50 I.Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD = ((S -D) /[(S+D)/2]} x 100 number (mg/L) (acceptable range = : 10%)Duplicate D o,0a 1. -----"- -1 Sample measurements: Sample Sample ED Sample characteristics Residual chlorine number B (shoud be < mg/) 0dd0113 ba 0 tI 0-L 1\ J f\ t" 0 16' ,' Di'o)4 L-o~b Note. All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.Laboratory control standard:[ Reference standard True value (TV) Measured value (MV) % RS -MV / TV x 100 number (mg/L) (mgIL) (acceptable range = 90 to 110%)I 1/15 3 +I-044flI I Reviewed by I ::* Date revieweda lage 60 of 107 E nvironmentallTesting Solutions, In.C D Page -5 1 Page_ " -of i Total Residual Chlorine (Orion Electrode Method, Orion 97-70)Matrix: Water, RL = 0.10 mg/L Meter: Accurnet Model AR25 pH/Ion Meter Analyst F t" r I77]Date analyzed LI5- II -E9i Iodide reagent: F I_/2 3 1 I Acid reagent: It% A 3 Calibration: 0.10 mg[L I 1.00 mg/L I[Reference standard number I XA(,S M 1 Tl.-S Mote: For samples with a residual chlorine of > 1.0 mgiL, the calibration range must be adjusted to bracket the chlorine levels of the samples.Laboratorv control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)-Z) 3 0.50 0, tif q4. e Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD= {(S -D) /[(S+D)/2I) x 100 number (mg/L) (acceptable range = .10%)-Duplicate r'.Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number mgIL Blank (should be <0.10 mg/L) '-ood7 t Oct ut%4. 01 ,1ýrk 6a " 33 ?'Y6 , qL, 1&Ij4 ~Oo 31 1o51'5.¶ -0 t S ' ?) IX C,16 d-io i Ab-,14 , e, -331 0-i -"%&ý 17f'+J, Ab, Mote. All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.age 61 of 107 Reviewed by. [-Date reviewed 0Page3L2 C 4 C~t age I of _L Environmental Testing Solutions, Inc.Total Residual Chlorine (Orion Electrode Method, Orion 97-70)Matrix: Water, RL = 0.10 mgiL Meter: Accumet Model AR25 pH/Ion Meter-Analyst L 7 7 Date analyzed oc-IL9_ Iodide reagent: --- 711337 Acid reagent: [cr_ 2DE Calibration: oo 0.1 mg[L 1.oo Reference standard number T NSSU-7.3 Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.Laboratorv control standard: Reference standard True-value (TV) Measured value (MV) % RS=MV/TVx 100 number (mg/L) (mg/L) (acceptable'range= 90 to 110%)"ThSb to'13 0.50 0.501 Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD ((S -D) /[(S+D)/21) x 100 number (mg/.) (acceptable range- +/- 10%)_.615, 0 S e- o.oco40-4 .Duplicate D LO.000113 -.Sample measurements: Sample Sample lID Sample characteristics Residual chlorine number .- (mg)Blank (should be = <0.7-mg7 40-.O.012-.9 M 1U .o 4 1 tai ih CLci ctMfo , Cn l0orY o.-0344 O 96qj-Di .-Scr 1-fi t ttfs VIktqto tn Y,. L-"C'.DlStSrcoId-kJ. RC)4 s\.9V+/-1djtou pa~~ot~fb LO.ootsI, Q~~~~~~ ~ ~ ~

OG I, (., d S0iIA0AN -k loidai , PCH , ,.W 0Lio4 ,. 3 o d N C') C-0 0,- C0o.o0X gL 0, 00711 IOct ____V ýO rl-O I-~ SA dooj-e- +&o~ ~.O.Note: All samples were analyzed in excess of EPArecommended holding time (15 minutes) unless otherwise noted.Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (rg/L) (Mg/L) (acceptable range= 90 to 110%)T__S.ci- "f t
0.50 OL .6 q 2.0 V/.Reviewed by Date reviewed LtO'-2rr -43 Iage 62 of 107 "ErS" EnvivnmentalTesting Solutions, Inc.Page 32.Page 3- of Analyst I tIII7-7 ,Date analyzed 0 --j]Titrant norm ality and multiplier determination Alkalinity (SN2320 B)Matrix: WtCe, RIL = 1.0 mg CaCO 3/L Titrate samples to pH = 4.50 S.U.Time initiated

" Time completed EIitIZZ1 pH of Normality Normality (N) of H 2 S0 4 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na 2 CO 3 x 0.05)/E = (Nx 50000)/ 100 ml sample water reference standard ml ml ml 0 0.25/E = Nx 500=4.5 S.U. number number (E) (acceptable range = 0.0180 -0.0220)-a -.-.-10 Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV! TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3 IL) (acceptable range (mg CaCO3/L) (ml) ml ml ml =90 to 1.10%)ITS 'S 454 100 100 11.'4 'o a 10.1 -'LOq Duplicate sam le precision: Sample Alkalinity %RPD =Sample Sample ID volume Begin End Total Multiplier (mg CaCO3VL) ((S -D) /[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range

10%)06-o t 4-o)M M1R", k4 too OXc co C5S U.-I sn g a Duplicate (B) D Matrix spike recovery: Referencestandard Spike value Sample Spike alkalinity (A)number (SV) volume Begin End Total Multiplier (lig CaCO 3 IL)(mg CaCO 3/L) (m[) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO3/L) MV = A -B (acceptable range (mg CaCO 3/L) = 75 to 125%)Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOgL)o ,o -bcit) f S 0O 7,10L Q).-+b!_ -,3Uo6S-0 -~ 2 -A___ ____05.oS-0 oq'q tn_ -., ,, _ 2 b~LY~__ I~~35_~0 1 6 0i W_______c~ ',4. 4. )CIO_ __I I age 63 of 107 Reviewed by: D arvw:s.Date reviewed: I t05A i S'1Envi..nmtnt Tolg Solutions. Inc.Page 83 Page c-2 ofL Time initiated Time completed Analyst I~s]Date analyzed mliier Titrant normality and m ultiplier determinationi Alkalinity (SK,2320 B)Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH -4.50 S.U.p 1 of D Normality (N) of H 2 S0 4 pH Factor or Multiplier Deionized Titrant check egin I = (5 ml Na2CO 3 x 0.05)/E = (Nx 50000)/ 100 ml sample water .reference standard ml ml ml Nx 500= 4.5 S.U. number number (E) (acceptable range -0.0180 -0. 7t Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS MV / TV X 100 number (TV) volume Begin End Total Multiplier (rag CaCO 3 IL) (acceptable range (mg CaCO 3/L) (mi) ml ml ml =90 to 110%),oss f 4 100 100 340 43.4 .4 1-,.f'Duplicate sam ple precision: Sample Alkalinity %RPD =Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) (S -D) /[(S+D)!21) x.100 number -_(ml) ml ml ml (acceptable range

10%)4 Duplicate,(B) s D 1..t .--w Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml). ml ml ml Multiplier (mg CaCOAL)D-cnocO 144 T 1V 10 bQe ______4 -3 (N~A A-, .OL -ý , G- __________ -Iý___L)). I ý 'b091'14 rA t .IN-, _ _ __ _n 6&MII gh AS n-1 i (ý -1 I-~ ~~~~~~~ ______________ I_______ t,__ _ _ i- -- I i~ ------^C4, nS ,Aq. 04 S_-5* .77'.. ýX-r, .' I oil r9 0'- -PH I e 64 of (2P~ Y 4 t I Al IOA,90 ..-ftl I tpD yi..J.~. L '-~'~-*'~

-' -J 107 Reviewed by: : Date reviewed:

OOQ Page _____: rEmnmontaJ TIN5l1. 1- In"c.lv Analyst[ 7 t Date analyzed -Titrant normality and multipier determination: Alkalinity (SM 2320 B)latrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH = 4.50 S.U.Page 3of-6 Time initiated Time completed pH of N -Normality (IV) of HZS0 4 pH Factor or Multiplier Deionized Titrant check Begin a9--_._ 5 ml Na2CO3 x 0.05)/E (N x 50000)/ 100 ml sample water reference standard ml ml ml 0 N-x 500 4.5 S.U. number number (E) '(acceptable range= 0.0180- 0.0220)Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (mg CaCO 3/L) (ml) nml ml ml = 90 to 110%)T S , 64 100 100 t)u, .q-, .'- I94 10I C, Duplicate sa le precision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3fL) ((S -D) /[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range=- 10%)Duplicate (B) 4, D, ___,.~f~ '__- -:Tto trPto Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A)number (SV) volume Begin End Total Multiplier (mg CaCO 3 IL)(mag CaCO 3/L) (ml) ml ml Sample alkalinity (B) Measured spike value (MV) % R MV SV x 100 (mg CaCO 3 1L) MV = A -B (acceptable range (mg CaCO31L) = 75 to 125%)Sample measurements: Sample volume

Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mag CaCO3IL)o0kos0 ,ol ( q 07.:S -D + L_,_,o_,_

,._÷ _i A , --o- -.., .I Reviewed by: z~IIj Reviwedby:Date reviewed.: 1C5 - Em" T jRlons. Nov SEnvinmnmentalTes'n Solut Iam.,Inc.Page Page 4o.if'Analyst pS-Date analyzed 05 Z 0-bi Alkalinity (_SMi320 B)Matrix: Waier7RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.50 S.U.Time initiated [ CI77]Time completed Titrant normality and multiplier determination: of Normality Normality (AI) of H 2 SO 4 pH Factor or Multiplier Deionized c-----cht,-L. Begin End Total =(5 ml Na 2 CO 3 x 0.05)/E = (Nx 50000)/100 ml sample water reference standard ml- = 0.25/E = N x 500= 4.5 S.U. number number (E) -______ ___ acce180- 0.0220)Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml =90 to 110%)[ Z s % -1 0 0 1 0 0 ...:'Duplicate sam lie precision: Sample Alkalinity %RPD =Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) ((S -D)/[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range = : h0%)Duplicate (B) 2 oL D Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A)number (SV) volume Begin End Total Multiplier (mg CaCO3/L)(Mg CaCO 3/L) (ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R = MV ISV x 100 (mg CaCO/L) MV = A -B (acceptable range (Mg CaCO 3/L) = 75 to 125%)Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml mi ml Multiplier (mg CaCO 3/L)o'~a .og -pCAI3Lr= $ 3 \b' t o 00, 0 --. 3. 5.3 .1.J).4i I .10 ll\65 0 D~S 02R __ 0__ 1 A40.V5 ~ 1CO ~III o0t0,;o *S 0"O'STVA-weri j I g 1 .50to -16'1,1 g I I : ,.'1/291all:CA oSoa. I(4 J I- 4 4 I + 4- 4~ +/-o0 oCLs oq.O 1/- tA ) in LrI4~L~.A -XI-1 lage 66 of-J -______________ ~---.~' I 07 Reviewed by: [II EII]Date reviewed: [ ? -ih o tý 'PPage .O Page_ 6 _5 l. (-of Analyst .Date analyzed L,ýAlkalinity (SM 232o B)Matrix: Wate-rkL = 1.0 mg CaCO 3/L Titrate samples to pH = 4.50 S.U.Time initiated [ I Time completed Titrant normality and multinlier determination: p Normality Normality (N) of H 2 S0 4 pH Factor or Multiplier Deionized Titrant --Begi _nd Total (5 ml Na 2 CO 3 x 0.05)/E = (Nx 50000)!100 ml sample water reference standard ml ml m- 25/E =Nx 500=4.5 S.U. number number (E) (acceptable range= 0.018 Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (mg CaCO 3 IL) (ml) ml ml ml =90 to 110%)I ss* 4-100 100 (g ,'.h, cl_ .4L Duplicate sam le precision: Sample Alkalinity, %RPD =Sample Sample ID volume Begin End Total Multiplier (Ing CaCO 3/L) [(S -D) /[(S+D)t21l x 100 number (ml) ml ml ml (acceptable range = +/- 10%)Duplicate (B) OD U ,.Matrix spike recovery: Reference standard Spike value Sample 'Spike alkalinity (A)number (SV) volume Begin End Total Multiplier (rag CaCO 3/L)(mg CaCO 3/L) (ml) ml ml ml -Sample alkalinity (B) Measured spike value (MV) %R=MV/ SV x 100 (mg CaCO 3/L) MV = A-B (acceptable range (mg CaCO 3 1L) 75 to 125%)Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (Ing CaCO 3/L)"T-VAIW6n 60 3o.r a,!5 1 .I-) LI,"4 q9*015 2 ___4 (-- iL a 1U&03 2,9'S 43_.3__12 DcO, o.t 2-. 1 1 I 0', OSO,!..O ,/' B l 44,-5.. -1 .-z ,CP bot '1A4.c Q -1 Wfb Ou-y 15Dt .t 0..... ,, --.-m I * -.~ I -~ ~ I I 0 .9 I. I 4 4 0 11 ~Lj.13 4-I I age 67 of I U -j -...07 Reviewed by: " I IZD w D ate reviewed: ( -l Oe Page ___Page. of Lo, Analyst [757- ]Date analyzed 10-bq Titrant normality and multiplier determination: Alkalinity (SMW320 B)4atrix: Waefr-td, = 1.0mg CaCO 3/L Titrate samples to pH = 4.50 S.U.Time initiated [ [Time completed -" I '-t-Normalit Normality (N) of 14 2 S0 4 pH Factor or Multiplier Deionized Titrant check Begin ---I Na 3 X 0.05)/E (Nx 50000)! 100 ml sample wer reference standard ml ml ml 5 -N x500= 4.5 S.U. number number (E) (acceptable range = 0.0180 -0.0220)Laborator control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3 IL) (acceptable range (mag CaCO3IL) ml) ml ml ml =90 to 110%)Duplicate sam le precision: .Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO31L) ((S -D) /[(S+D)/2I) x 100 number (ml) ml ml ml (acceptable range =

10%)Duplicate (B) -D Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A)number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)(mg CaCO3fL) (ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 1.00 (rag CaCO 3/L) MV = A -B (acceptable range (mng CaCO 3/L) =75 to 125%)Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3IL)oosot."D 1-21670J 2 62 I 0 l b-i o'-'O*q( SU. O's i -504- -bi2- Q.5~ Wi_____o'ic~1.s n- ~ ' W- 9 2sp0 114 0 39y,} qq~o'ip~~cfl.O10 % 0 jj j P 00~js-o1.o¶A nAA V r%,, 10O Q-ý 3 159 I ______ I t 1.

I I I -1.9sbl _l'age 68 of& ..&107 Reviewed by: IZýý Z Date reviewed:

F7 0 Z 0* ~ -Page ____Page 1 of- 3 Analyst 7 Date analyzed Lii. J Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH = 4.50 S.U.Time initiated f 7&.Time completed T,~fr,,n' normality and multinlier determination:-t a no ..... _ __ .... .. .. .de ....... ...pH of Normality Normality (A) of H 2 S0 4 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na 2 CO 3 x 0.05)/E (N x 50000)!100 mr!sample water reference standard ml ml ml = 0.25/E = N x 500= 4.5 S.U. number number (E) (acceptable range = 0.0 180 -0.0220)6.0 IN mF3,9 T-1,.S kp,53 t 1. ,L, IIP 1U..09.rT _ _ _.c_Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (Mg CaCO 3/L) (acceptable range (mg CaCO 3 IL) (ml) ml mi ml = 90 to 110%)94100 i~ 1L RO -Duplicate sam le precision: Sample Alkalinity %RPD =Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) ((S -D)/I(S+D)/21) x 100 number (ml) ml ml ml (acceptable range = + 10%)fU o ., I4 s tc as -2Xjo' MKS WIG2 100 C,.3 9 ~5 .5 10J01 S l-0C'4 Duplicate (B) __ .- D Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A)number (SV) volume Begin End Total Multiplier (rag CaCO 3 IL)[(mg CaCO 3 IL) (ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO3fL) MV = A -B (acceptable range (mg CaCO 3 L) = 75 to 125%)(c0 4:"".,. 50 Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (rmg CaCOV/L)0643.Q5 IC .q _ _ _-I" O 4-4 ija-5( 5~ U_ _ ____ __ __ __ __ __ ____ ___~5~ 1. ~4 _ __0S- %'z -GP-06-1 zor 4 I1.01 0q-+~~ ~~~~ L4-~ _ __ ---~---I q-C-' rr--,A I I J'......44 _ k.... ..H-Q-+ I*=~-~------+ ~4 4 ~ 4 -, \T) AI-L,7O n,. .' , -, I .I£ ~ ~ ý7"' 14i"I i 4J4TL-'(OI.P4A A .--L' 2.< -2-fC-k :)4- 3-1+----+ , 1 lage 69 of I Q ~ ~ i~ L,+/-1.-~- -39 07 Reviewed by: Date reviewed: [ -[: ~,' Enironmn~,tA T"tIg Soluions, Inc.Page 39 Page c of. 3 Analyst Date analyzed 0 q.o"-Titrant normality and multiplier determination: Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH' = 4.50 S.U.Time initiated Time completed pHo 6T Normality (N) of H 2 S0 4 pH Factor or Multiplier Deionized Titrant check e Total = (5 ml Na 2 CO 3 x 0.05)/E = (N x 50000)/ 100 ml sample water reference standard ml ml ml-- -= .5/E = Nx 500 4.5 S.U. number number (E) (acceptable range = 0. -ýLaboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV/TV x 100 1 number (TV) volume Begin End Total Multiplier (mag CaCOgL) (acceptable range (Mg CaCO 3/L) (mI) ml ml ml =90 to 110%)* 100 100 b.o 0 q.4 -it).q Co_ 42o0p Duplicate sa ple precision: Sample Alkalinity %RPD =Sample Sample ID volume Begin End Total Multiplier (mg CaCOyL) {(S -D) /[(S+D)/21} x 100 number (m_) ml ml ml (acceptable range = +/-10%)Duplicate (B) D Matrix spike recovery: _Reference standard Spike value Sample 'Spike alkalinity (A)number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)(rg CaCO3/1L) (ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R =MV/SV x 100 (Mg CaCO 3/L) MV = A -B (acceptable range (mg CaCO 3/L) = 75 to 125%)Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (Mg CaCO 3 1L)0or OS106 l a 3c' 1i ~ SIC' -ý ~.1 17.? 4-0 44____0i1L 1 b'n I -A-Stwkk 60t 1: .ý -.IL 3L. -O I QXlasbh O Oc b Ic~a .0-L.i -I 2 ,h I ,QA,. (-i*~ ~ ~ ~ ~~Z I ~ ~ 2~J 44 --1 _5 I I WA-56aciT ..Afý C'51-t-+ -4 4--F~'-~--=-~ q ewjOs OSii. Q0 i S 7 I F'4 7 -----I -44------ I -Z I , k' a^ ý I I ~1-fl-'..--F~-~-'d--4s-F -~I 4 I I 4- I I "- I * 'I ' I I 41i --I---r. f .I it4 -, i A 1 -)1: I --.C'a, .0 8 2'I.JC)+L4 A 2-* b of 107 Reviewed by: Date reviewed: -i f4 -0 9 Ennkonment.I Tescin~ SnI,.tiws. Inc.Page 1-"*Page 3 of 3 Analyst 7 c [Date analyzed .IG ,,-I+ -Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH = 4.50 S.U.Time initiated Time completed Duplicate sam ple precision: Sample Alkalinity %RPD =Sample Sample ID, volume Begin' End Total Multiplier (Ing CaCO 3/L) ((S -D) /[(S+D)/21) x 100 number IVA (ml) ml ml ml I .(acceptable range = : 10%)Duplicate (B) D .24o D Matrix spike recovery: Reference standard *Spike value Sample Spike alkalinity (A)number (SV) volume Begin End Total Multiplier '(Ing CaCO 3/L)(mg CaCO 3/L) (mi) ml ml ml -50 11. ).-q. ,. i.q150 Sample alkalinity (B) Measured spike value (MV) % R = MV / SV X 100 (mg CaCOjfL) MV= A-B (acceptable range (mg CaCO 3 IL) 75 to 125%)L) allg 04 e 71 of 107 Reviewed by: Date reviewed: -iiLOZ E , Testnqg lutis. Inc.Page ___9 Page Il of 6 Total Hardness (SM 2340 C)RL= 1.0gg CaCO0 3 L Analyst fZ_ 77E Date analyzed o- -Time initiated t:q 0 Time completed Titrant normality and multiplier determination: Titrant Normality check Begin End reference standard I ml ml pH Factor or Multiplier = (I x 50000)/50 ml sample=Nx 1000 I (acceptable range = 0.0180 -0.0220)I T) ~nI.,f a, to " 10p nrO',1*CM"* Sample Hardness %RPD =Sample Sample ED volume Begin End Total Multiplier (mg CaCO 3/L) ((S -D) /[(S+D)/2]} x 100 number (ml) ml ml ml C)5.d-Cn&~ fydis 7 X) 0O 50 D I(o. L .4-k' 2 S q Duplicate (B) ---D Matrix spike recovery: __Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (mg CaCOj/L)(Mg CaCO 3/L) "(M) ml ml ml Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (amg CaCO3/L) MV = A -B (acceptable range (mg CaCO 3 L) 75 to 125%)Sampe measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (frg CaCO 3/L)TV = N Blank (should be = 0 rg CaCO3/L) 23 -5. _ 5 C.)v5o,01oo6 T 8 I S -wbS0 R D 6ii JLL _ _I~II~I~ _ 3I-45 44_____I uzý -U 0 "11 I Uz~ U90c211

F + -~--~-- F -~---~- F --'-- 4 ---------

4 --, I- ~ ~ I F -~ +,-~4I~-I--.---f---~-.----f------4---------4 .-~L41ýýý'I-1 t+ý li i + ; -Nbe-w--.... .-+ 4-'~ ALL-L l fo' i9- t j u sa l e tel u t age -If >1 5ml of titrant is used, sample must be diluted.12-(3-h h --Reviewed by: Date reviewed ! ( 0,0t E 9=4u19ln Page Page of, Total Hardness (SM 2340 C)RL 1.0 m CaCO 3/L Analyst[7!Z77.. ]Date analyzed L Ij- 13 ij.Time initiatec I Time completed [IiýII Titrant normality and multiplier determination: J I TitraL_ Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference stanuderd --)ml ac = 0.2/1E 00 (N x 50000)150 ml sample number numbe (E)' ac = 0-0.0220) =Nx 1000[ (Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (mg CaCO3IL) (ml) ml ml ml =90 to 110%)~j$ ~40 10 4a2~o Duplicate sam le precision: Sample Hardness %RFD =Sample Sample ID volume Begin End Total Multiplier (ing CaCO 3 IL) A(S -D) /[(S+D)/2]) x 100 number (ml) ml ml ml 1 0 ' 7 J ý _ _ _ _ _ _ _ _ _/ Duplicate ( ).L .. .- 3 .... D L "r7______ ______31 1_ 3.3 LU Matrix spike recovery: "__Reference standard Spike value Sample Spike hardness (A)number (SV) volume 'Begin End Total Multiplier (mg CaCO3,L)(mg CaCO3/L) m) m ml ml Sample hardness (B) Measured spike value (MV) % R MV SV x 100 (mg CaCO 3/L) MV= A-B (acceptable range (mg CaCOIL) = 75 to 125%)Sample measurements: -Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mrg CaCO 3/L) Blank 0o01 -. Av Z o -2 3. 20 01hLlz O's car 0 -LL 23 ~. j 1OS 4.Q~1 .0- _____ L-. ' 2 4 2,2_5 o1osU,,o / 2. __ t4 __._5 _5C t-,.-a-f Qt! I 0.0 13 A J __________ 0 --1! 1z& k- G.,7-4 5 i LAL-L~~7 L.{L4 I ..I I. I- 4 * ~ ~ j. I'-4 1 -~ 4 ~-,.-------4----~---

4 .~ I os-OS-01NA I 1-1 U; 0[age 73.13 ._4- I tL F5 of If >15ml oftitrant is used, sample must be diluted. Reviewed by: .I i I Date reviewed[ 0L D -01 1

'a SEn lonment eI Testlng Solution.. Inc.Page To__Page __. of C(.Total Hardness (SM 2340 C)'R= 1.0._Mg CaCO 3/L D a yste ....Date analyzed , (-,c-10 ".o q Time initiated Time completed I Titrant normality and multitwler determination: Titran -k Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard In ml = 0.21E (N x 50000)/ 50 ml sample number number ce table range 0.0180 -0.0220) = Nx 1000 Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS = MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (mg CaCO 3/L) (ml) mJ ml ml = 90 to 110%)40 50 ..2- c. 2-0.0 4ot0" Duplicate sam le precision: Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (Ing CaCO3/L) {(S -D) /[(S+D)/21} x 100 number .(ml) ml ml ml . avtt 2s __. ._-_* t Duplicate (B) 4.9 q-2 -I ID r ~~Matrix spike recovery: ." Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (mng CaCO 3 1L)(mg CaCO 3 1L) (ml) 0 ml ml F)S Stp t41-12 2-0Z Z Sample.hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO 3/L) MV = A -B (acceptable range (mg CaCO3/L) 75-to 125%)Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID ( , ml ml ml Multiplier .(rg CaCOJL)TV = NDBln TV ~ ~ = Do-uld be = 0 mn CaCO6/L).Au ,n-- iqt 1- Ft 35 4 A , 2c).o 110 orOs , S.O\ 0 2 00 ,_ AO3 42 0 (y T 3.1 1, o -1 OS 61o. _ iL I t'A~sC.OE. OR'12,14 Il~~~WA~_r _ _ I_2_ [ I t. .L Jý I gage 74 of Wo:if>15ml of titrant is used, sample must be diluted. Reviewed by: I I Date reviewed [ 0 -Is.0l{- 4 innlonmenta Tmtin alurbtons. Inc.Page _'W Total Hardness (SM 2340 C)RL = 1.0 mrg CaCO/L Analyst[ .7J --Date analyzed j '-5j- j]Titrant normality and multiplier determination: Page _ of Time initiated F77771 Time completed EZI ._Tit Normality check Begin End Total Normality (N) of EDTA. pH Factor or Multiplier reference standArd- -a-r- ---mI- ml = 0.2/E = (N x 50000)/50 ml sample number number ac---aceptable range = 0.0180 -0.0220) = Nx 1000 Laboratory control standard: .__ __Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number '(TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO 3/L) (ml

ml ml ml =90 to 110%)Xl" 6040 50aiVd0. 002 Duplicate sam ple precision:

Sample Hardness %RPD =Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3 JL) {(S -D) /[(S+D)/21) x 100 number (ml) ml ml ml OR Os 06-'LS 7VA UtP~ -?., -ý7 L,11 _____O Duplicate (B) .- .,___i.Matrix spike recovery: _Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (mg CaCO 3 IL)(mg CaCO 3/L) (ml) ml ml ml Sample hardness (B) Measured spike value (MV) % R= MV / SV x 100 (mg CaCO 3/L) MV = A -B (acceptable range (rag CaCO3/L) =75 to 125%)Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)TV=2DBlank Vg mCO/L)._OcsoS-Oc ._,__._ 7-I.Co 4-o 001 0-506--Z( 2-~f~o n0 ~ 3 q _ ___5i 0__ __ 2Sý re__ __ fl4 pCiUSQ.I'0 -NA___ ~ ~ 1 _ __ _ _ _ _ _Or,fln, ii t i 2 1.i 14's.314 Z -4 ++ -I .1 .1- 4 --.--.-.~~----i--~....-----i---..----------i g "I l**t~~)ngý 0e, nk. It I.- JV--)----4 + +----f-~--+-~--'-'----4---------~ +n CQ q0:[/A W&V11Jl7~-W) Q YýL-Li i110 -11L 0A2 ~III?~ It I I age 75 of 107 Note: If >l5nmd of titrant is used, sample must be diluted.Reviewed by: i I reiw-/ I Date reviewed] 0, 'iO - I I]I~i I Page 9_ _Page of Total Hardness (SM 2340 C)RL = 1.0:mg CaCO 3 L Analyst andttderia Date analyzed o -mi~iiiq Time initiated Time completed LI Titrant normality and multiplier deter mination: I iran I !End Total Normality (1) of EDTA pH Factor or Multiplier reference standard ml ml = 0.2/E (Nx 50000)/50 ml sample number number (E) (accepta e -0.0220) = NxI 1000 Laboratory control standard.Reference standard True value Sample Hardness (MV) % RS = MV / TV x100 number (TV) volume Begin End Total Multiplier (mg CaCOj/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml =90 to 110%)* 40 50 3 ~5 e. ~ 4 o 0;Duplicate sample precision: _Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3 IL) ((S -D) /[(S+D)/2]} x 100 number (ml) ml ml mln[O(6 01 I2' 4A/ )% P ILW I S ___Duplicate.(B) D 4 Matrix spike recovery: ____Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (mg CaCO 3 IL)(mg CaCO 3 IL) (ml) ml :ml ml Dý%(q J-4.14~ ITh.'Sample hardness (B) Measured spike value (MV) % R MV / SV x 100 (mag CaCO3/L) MV = A -B (acceptable range (mg CaCO 3/L) = 75 to 125%)Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (mI) ml ml ml Multiplier (mg CaCO3IL)TV =,l)Blank 004 N oo_.. 01_ _- -,4 2-09t 0ofo. 12-- :3 L201.o 131.9 :q,,3 fv? 92-,,,A osoL, .o(.7/A lU4~k~ijI2.,n-fdSl piq N.q 01 I q OS04 -of- 71A M- týdiirrfi)Jl 1 '31 q I 7 i 1): (Z, kage 76 3 Qri TI..)-of WEo: If >15ml of titrant is used, sample must be diluted. Reviewed by: Date reviewed [ -P) .LfA . Envtonmn rntal TIrg 5.lutions, Inc.Page '-ý'Page C,2. of(_O Total Hardness (SM 2340 C)RL = L1O,0mg CaCO 3/L Analyst [%,7....Date analyzed Time initiated I I Time completed 1 TitraizLnozmalltv and multinlier determination: Titrant Normality.check Begin- -n Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml N */500 /50 ml sample number number (E) (acceptable range = 0.0180 -0.0220) x 1000 Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS=MV/TVx100 number (TV) volume Begin End Total Multiplier (mg CaCO 3 IL) (acceptable range (mg CaCO3/L) (m4) mI ml ml 90 to 110%).13 ( ~- 40 50o 2o.0 -,o Duplicate samr pie precision:" Sample Hardness %RPD =Sample Sample ID volume Begin End Total Multiplier (mag CaCOfL) ((S -D) /[(S+D)/21) x 100 number (ml) ml ml ml o'io nusclo EI~iTn oo 145' 3Co 2- 3q 1 33.~-t LI Q S Duplicate (B) ---, -" D /Matrix spike recovery: "____Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (Mg CaCO 3 L)(Mg CaCO 3/L) (ml) ml ml ml 4z.,, 6 S (7) -'0b 0a Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mng CaCO 3/L) MV = A -B (acceptable range (mag CaCO 3/L) = 75 to 125%)Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO 3/L)TV = 14D- Blank _4L (should be = 0 mg CaCO3/L),)tfl.oL. EIY vp 001 5"0 Mo )24 .i.A )-o.o O', osal. 1 T 0o2. /0 NA j. 1 q 5) __ 10 OS -O EM J, 2 _-_--, if "'-_ _ _ _ _ _~~~~ _ _ _ _ _ _ _ _ _ _ i -1 t~~t Sage 77 of IoTte: If >15ml of titrant is used, sample must be diluted. Reviewed by:t(,4j,. I1 Date reviewed Ii Orý3?iiI Yl Page ___M Page :1- of 3 Total Hardness (SM 2340 C)RL = 1.0 mg CaCO 3/L Analyst bs...Date analyzed 'Time initiated [Dy:-i Time completed I ý,.S Titrant normality and multinlier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml = 0.2/E = (N x 50000)/50 ml sample number number (E) (acceptable range = 0.0180 -0.0220) = Nx 1000 Laboratory control standard: Reference standard True value Sample* Hardness (MV) % RS = WV / TV x 100 number (TV) volume Begin End Total Multiplier (mMg CaCOVL) (acceptable range (Ing CaCO3fL) (ml) ml ml ml =90 to 110%)-TWI6 40 50 i).0 12U -e, U, f40 Duplicate sam te precision: "__Sample Hardness %RPD =Sample Sample ID volume Begin End Total Multiplier (nmg CaCO 3 IL) {(S -D) /[(S+D)/2]) x 100 number (ml) ml ml ml O*2ti ji'-. I F S " -.- .....S Duplicate (B) .-I =1,5 Jt. q .,- .D C1 2- : Matrix spike recovery:._ Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (mg CaCO 3 JL)...._ .(mg CaCOj/L) (ml) ml ml ml________ ______ 50 52 , Q5 Io -to%)Sample hardness (B) Measured spike value (MV) % R =MV / SV x 100 (mg CaCO 3/L) MV= A -B (acceptable range.(rmg CaCO3/L) =75 to 125%)92- Sample measurements:,- Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (rag CaCO 3/L)TV=ND Blanko 6S.oqo9A- --,s L) U 4Fb'-4,OD-', J __ ___ 4 t L.C 1ILq OS _ __ ._- 0!'I -1 I'I-'.--J I ~S~L~zz I I -2~ t ~ +/-+/-_ I. ~=~= I 1-tge 78 of 10hte: If >15ml of titrant is used, sample must be diluted.Reviewed by: Date1reiewed Date reviewedI Z Q-f "0 sInc.Page _q6 Page 3of 3 Analyst f --.Date analyzed -II Titrant normality and multiplier determination: Total Hardness (SM 2340 C)RL = 1.0 mg CaCO 3/L Time initiated. 'Tinme completed I-NQ-rmalitY check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standar- ------- .ml ml = 0/E -(N x 50000)/50 ml sample I number number -- ( _._acceptable range= 0.0180 -0.0220) = N x 1000\ I Laboratory control standard: .Reference standard True value Sample Hardness (MV) % RS = M / TV x 100 number (TV) volume Begin End Total Multiplier (Mg CaCOj/L) (acceptable range (rngCaCO3/L) (ml) ml ml ml =90 to 110%)17%--f 40 50 Gt `6 4-' Q- 2D.0 ~ c Duplicate sam le precision:-.. Sample Hardness %RPD =Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) {(S -D) /[(S+D)/2]} x 100 number (ml) ml mil ml oicsik-ol 7-A.swlo. ____) )4-1ý 19c, 3 2-D-O Duplicate (B) D Matrix spike recover:.Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)(mg CaCO3/L) (ml) ml ml ml Sample hardness (B) Measured spike value (MV) % R =MV /SV x 100 (mg CaCO 3/L) MV= A -B (acceptable range (mrg CaCO 3/L) = 75 to 125%)\o4-h Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (Ing CaCO 3/L)TV =N -t.- ' n ,k-(should be =0 rg CaCO __L)11 .- of iq% 3oo9 z 3~a.5 3,5,. .%i 041 O S -s .-3 Q- -.maotoiA- toto 4 39-,+ LL 3=.o0 o% a .--l o PL 4 J,%- j I ,__ b.t 3 3.3 lage 79 of 1 Wte: If >In5ml oftitrant is used, sample must be diluted. Reviewed by: ---I (1 -)Ift e -411__1 ~-I II i.-------4 Date reviewed D v 5- nr+- Page I"/I Page 3 of 3 Total Hardness (SM 2340 C)RE = 1.0 mg CaCO 3/L Analyst [g_ .Date analyzed LI Time initiated Time completed Laboratorv control standard: Reference standard True value Sample Hardness (MV) % RS = MV / TVx 100 number (TV) volume Begin End Total Multiplier (rag CaCO 3/L) (acceptable range (rag CaCO 3/L) (ml) ml ml ml 90 to 110%)ING:S. W toS 40 50 3.3 5".-4 C. 2-73 ýO lb i5C-L Duplicate sam ple precision: Sample Hardness %RPD =Sample Sample ID volume Begin End Total Multiplier (rag CaCO 3/L) {(S -D) /[(S+D)/21) x 100 number -'_[ (ml) ml ml ml ClqO~lb-,oi 15(aý tYOQ 2- 6-D .5 3.t 1 .. .-Duplicate (B) " [ g ".- Matrix spike recovery: __Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (mg CaCOVL).(mg CaCOVL) (ml) ml ml ml 0 5D-- ,.5 17, q- .aIC)0 Sample hardness (B) Measured spike value (MV) % R = MV / SV x. 100 (rag CaCO 3 IL) MV.= A -B (acceptable range (mg CaCO 3 1L) = 75 to 125%)Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (m) " ml ml ml Multiplier (rmg CaCO 3/L)T=NDBlank.I-TV__= ___(should be = 0 mg CaCO 3 IL)-#ooSo,. LX ..D I g --z oios(~.I 4 ~ .4~ l~ (' _____ ________4- f-=-~---4 4 5 4.-I I-4 *4 4 4--. L ~ -4 _______________________ _______________ I .h.. ....L I t ge 80 of ,, V o. Date r e: ge8 ol~i~e: If >l5ml oftitrant is used, sample must be diluted. Reviewed by: Z. i.__Date reviewed Sequoyah Nuclear Plant Biomonitoring May 12-19,. 2009 Appendix D Reference Toxicant Test and Control Chart Page 81 of 107 Pimep hales promelas Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc.Environmental Testing Solutions, Inc.1 9 .. .I I I I I I I I I I I I I USEPA Control Limits (+/- 2 Standard Deviations) i i i 1.0 F-0.8 0.6 0.4 1.2 I I I I ; 1 1 1 1 1 ' I I I. I I I I I I I W arnI I I I I I I I I I I I I I I USEPA .Warning and ContivotLimits (7,5 ,,and 9 Oth Percentile CVs)....Qn eq P=-lcI 1.0 0.8 0.6...........I ...............I ...........I .......I ........................0.4 H I I I I I ~ I I I I I I I I I I I 1.2 1.0 0.8 i I l I i I I I I I I I I I I I I I Laboratory Warning and Control Limits. (10 and 25 Percentile CVs)S I I I I I i I I I I I .* i I 0.6 0.4 Test date 7-day IC 2 s = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC 2 5)Warning Limits (mean IC 2 5 -SA1 0 or SA.75)Control Limits (mean IC 2 5+/- SA.251 SA.90, or 2 Standard Deviations) ETS 4 Environmental Testing Solutions, Inc.Test number Test date 7-day IC .CT (&'L KCI) (gfl, KCI)Pimephales promelas Chronic Reference Toxicant Control Chart State and USEPA Laboratory S Control Limits S&.0 Warning Limits SA.2 CT -2S CT+2S CT-SAt.1 CT+SA.1 0 Laboratory Control Limits CT - 5 CT + SA.as USEPA SA.7 5 Warning Limits SA.tO CT -SA75 CT + SA.s USEPA Control Limits CT -SA.,o CT + S,90 CV i 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 I8 19 20 10-28-08 11-04-08 12-09-08 01-06-09 01-13-09 01-15-09 01-21-09 02-03-09 02-10-09 02-17-09 02-24-09 02-25-09 02-27-09 03-03-09 03-10-09 03-10-09 04-14-09 04-21'-09 05-05-09 05-12-09 0.79 0.67 0.69 0.76 0.82 0.77 0.78 0.79.0.83 0.53 0.60 0.76 0.72 0.75 0.79 0.77 0.70 0.80 0.69 0.75 0.73 0.72 0.73 0.75.0.75 0.75 0.76 0.77 0.74 0.73 0.73 0.73 0.73 0.74 0.74 0.74 0.74 0.74 0.74 0.08 0.06 0.05 0.66 0.06 0.05 0,05 0.05 0.09 0.10 0.09 0.09 0.08 0.08 0.08 0.08 0.08 0.08 0.07 0.56 0.90 0.09 0.64 0.82 0.59 0.84 0.09 0.63 0.80 0.62 0.84 0.09 064 0,81 0.62 0.87: 0.09 0.66 0.83 0.64 0.86 0.09 0.66 0.84 0.65 0.86 0.09 0.66 0.85 0.66 0.86 0.09 0.67 0,85 0.66

0.87 0.09 0.67 0.86 0.56 0.92 0.09 0.65 0.83 0.54 0.92 0.09 0.64 0.82 0.55 0.91 0.09 0.64 0.82 0.56 0.91 0.09 0.64 0.82 0.56 0.90 0.09 0.64 M 0.82 0.57 0.90 0.09 0.65 0.82., 0.58 0.90 0.09 0.65 0.83 0.58 0.89 0.09 0.65 0,82 0.59 0.89 0.09 0.65 0.83 0.59 0.89 0.09 0.65 0.83 0.59 0.89 0.09 0,65 0.83 0.15 0.15 0.15 0.16 0.16 0.16 0.16 0.16 0.16 0.15 0.15 0.15 0.15 0.15 0.16 0.15 0.16 0.15 0.15 0.58 0.57 0.57 0.59 0.59 0.60"0,60 0.61 0.59 0.58 0.58 0;58 0.58 0.58 0.58 0.58 0.58 0.58 0.58 0.88 0.87 0.88 0.90 0.91 0.91 0.92 0.93 0.90 0.88 0.89 0.88.0.89 0.89 0.89 0.89 0.90 0.89 0.89 0.28 0.27 0.28 0.28 0.29 0.29 0.29 0.29 0.28 0.28 0.28 0.28 0.28 0.28 0.28 0.28 0.28 0.28 0.28 0,45 0.45 0.45 0.46 0.47 0.47 0.47 0.48 0.46 0.45 0.45 0.45 0.45 0.46 0.46 0.46 0.46 0.46 0.46 1.01 0.33 0.99 0.32 1.00 0.33 1.03 0.34 1.04 0.34 1.04 0.34 1.05 0.34 1.06- 0.34 1.02 0.33 1.01 0.33 1.01 0.33 1.01 0.33 1.01 0.33 1.02 0.33 1.02 0.33 1.02 0.33 1.02 0.33 1.02 0.33 1.02 0.33 0.40 0.39 0.40 0.41 0.41 0.42 0.42 0.42 0.41 0.40 0.40 0.40 0.40 0.41 0.41 0.40 0.41 0.41 0.4]1,06 0.11 1.04 0.09 1.05 0.07 1.08 0.08 1.09 0.08 1.09 0.07 1.10 0.07 1.11 0.07 1.08 0.12 1.06 0.13 1.06 0.12 1.06 0.12 1.06 0.11 1.07 " 0.11 1:07 0.11 1.07 0.11 1.07 0.10 1.07 .0.10 1.07 0.10 Note: 7-d ICz 5 = 7-day 25% inhibition concentration.

An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. CT = Central tendency (mean IC 2 5).S = Standard deviation of the IC 2 5 values.Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and.warning limits recommended by USEPA for the test method and endpoint.SA.15 = Standard deviation corresponding to the I 0h percentile CV. (SAlo = 0.12)SA.5s = Standard deviation corresponding to the 25"' percentile CV. (SA 2s = 0.21)USEPA Control and Warning Limits S,75 = Standard deviation corresponding to the 75"' percentile CV. ( SA.75 = 0.38)S.,O =-Standard deviation corresponding to the 90"h percentile CV. (SA.0 = 0.45)CV= Coefficient of variation of the IC 2 5 values.USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applicalions Under the National Pollutert Discharge Elitnination Program. EPA-g33-R-00-O03. US Enviroamennntal Protection Agency, cimrw i, OH.Organisms obtaimfdtfrom Aquaio, Inc.atox05-12-09 -~ Precision of Endpoint Measurements , "IPimephales promelas I" Chronic Reference Toxicant DataEnvironmental Testing Solutions, Inc.Test number Control Test date Survival Control Mean Growth CT CV CT MSD PMSD CT for Control Growth (%) (mg/larvae) (mg/larvae) for Control Growth CV (%)(%) for PMSD (%)1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 10-28-08 11-04-08 12-09-08 01-06-09 01-13,09 01-15-09 01-21-09 02-03-09 02-10-09 02-17-09 02-24-09K 02-25-09 02-27-09 03-03-09 03-10-09 03-10-09 04-14-09 04-21-09 05-05-09 05-12-09 100 97.5 100 100 97.5 100 97.5 100.100 100 100 100 100 100 100 100 100 100 100 100 0.586 0.643 0.522 0.644 0.556 0.707 0.594 0.671 0.591 0.804 0.744 0.659 0.578 0.664 0.661 0.696.0.671 0.642 0.805 0.717 0.614 0.583 0.599 0.590 0.610 0.607 0.615 0.613 0.632 0.642 0.643 0.638 0.640 0.642 0.645 0.646 0.646 0.655 0.658 6.5 2.5 2.4 3.2 9.7 6.5 4.9 8.4 5.1 3.3 2.5 8.2 6.8 8.1 6.8 8.8 10.3 8.3 7.9 7.0 4.5 3.8 3.6 4.9 5.1 5.1 5.5 5.5 5.2 5.0 5.3 5.4 5.6 5.7 5.9 6.1 6.2 6.3 6.4 0.10 0.12 0.05 0.09 0.06 0.09 0.07 0.06 0.09 0.06 0.10 0.07 0.08 0.08 0.09 0.09 0.08 0.07 0.07 0.06 17.8 18.7 10.0 13.3 11.2 13.3 11.5 9.6 15.7 6.8 13.0: 11.1 13.1 12.3 12.9 12.6 11.4 11.2 8.4 8.9 18.2 15.5 14.9 14.2 14.1 13.7 13.2 13.5 12.8 12.8 12.7 12.7 12.7 12.7 12.7 12.6 12.5 12.3 12.1 Note: CV= Coefficient of variation for control growth'.Lower CV bound determined by USEPA (1 0th percentile) = 3.5%.Upper CV bound determined by USEPA (9 0 th percentile) = 20%MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.Lower PMSD bound determined by USEPA (10n percentile) = 12%.Upper PMSD bound determined by USEPA (90th percentile) 30%.CT Central Tendancy (mean Control Growth, CV, or PMSD)USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.Organisms obtained from Aquatox, Inc.Page 84 of 107 atox05- 12-09 "ETS Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source: Aquatox, Inc.0 C U Cu 0 o.J 0-S A I.a-Environmental Testing Solutions, Inc.1.25 I I 1.00 0.75 0.50 0.25 USE]I I I I 1 30 20 1.0 0-. -I I I I I I I I I ' I I I I I I I I I I Kentucky Acceptance Limit (< 30.0%)..... ........... ..... *. ............... ...................................................1.. ....30 ..USEPA Upper PMSD Bound (90"' percentile <30.0%)20 ~ ~ ~ ~ .. ...."............... 20.0+............. ........ ................................. 10 e It I I I I I I Test date---- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. -- -- Central Tendency (mean Control Growth, CV, or PMSD)ige 85of i 0 7 Control Limits (mean Control Growth, CV, or PMSD + 2 Standard Deviations)-j r 6 9 DSETS Page I of 5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)Species: Pimeph ales promelas PpKCICR Test Number: Dilution preparation information: Comments: KCI StockINSS number: It,ýS 6'k__Stock preparation: 50 g KCVL: Dissolve 50 g KCI in I-L Deionized water Dilution prep (mg/L) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume.(mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Test information: Organism age: "o. , .0 Randomizing template: I(e.2.Date and times organisms ' -o\ 0 boo Incubator number and were born between: shelf location: Organism source: fkmý 6 e Cs_ o, 0%. , Artemia CHM number: (A 4Lqq Transfer bowl information: pH = SU Temperature = C Date / Time in: 0 S-. jg- __q ___-1'. -4.-o Date/Time out: o-. 20-dk kZZo Average transfer volume: Total drying time: Z4. .S 4OJwC 0. 1"WC)S Initial oven temperature: 60" C, Final oven temperature: hi" ,..L Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW or termination batch used Time Analyst Time Analyst Time Analyst 2 os,-,A. eqto 1o .L OS-o 0 1 6 2 O aL.. 1 q1 0 C)t, IL a is'.L 1 6___OS-11-po L1. %Soo Os 1LZ nt ' 6kA 'id-n 2..mS11 7 OS-Iqi -o0 lS6.1 Control information: Acceptance criteria Summary of test endpoints: % Mortality: 0)7. _20% 7-day LC 5 0 9qq. 0 Average weight per initial larvae: _ 0,-11'"= NOEC (V bo Average weight per surviving larvae: 011 l "1 2 0.25 mg/larvae LOEC -SO ChV t,,'3 O. 8 IC 2 5-1-5. , ge 86 of 107 lETS Page 2 of 5 Species: Pimephales promelas PpKCICR Test Number: j_2-Survival and Growth Data Day Control 450 m KCI/L 600 mi KCI/L A B C D E F G H I J K L 0 to to/Q /Q0 /0 to0 10 to t /0 1 C)I0 tO 0 c /0 /0 /0 16 1) (3 ./0 /0 tO 2 10 '0 /0 O 0 /0 /0 /0 16( /1 /0 3 /0 ,O 1( O 11) ! I /0 /0 / 0 /0 /0 4f /0 O /0 t 0 1 0 /0 5 50 I / to 0 10 1 1O) /0 /0 /0 /0, /0 6 0 tO iO 10 (0 (O 10 t/0 0 10 /0 10 7/ /0o e0 10to o 1 t0 0 0 A = Pan weight (mg)Tray eolor code:: or .14 -.11 13.&-ý 14 3, 3 13.oD " 14.49 14 ,3T I}.3 1 .9 i t I ." .q ; q Analyst: _ npli Date: O- ofq;:-A'__ B = Pan +.Larvae weight Analyst: Date: bS *___11__ -Oak C = Larvae weight (mg)=B-A Weight per initial number of larvae (mg)= C / Initial number of larvae.3'V 0'4, N'-V I,/.0%Qa~1 N V tv O" N-z S.N 0'"2 N Average Percent weight per reduction initial from control 0111 number of (%)larvae (mag)_ _-1 -O~bbq .110 j I.O7." Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk sick, sm = unusually small, I Ig = unusually large, d&r decanted and returned, w wounded.Calculations and data re Comments: age 87 of 107 eviewed: D, Page 3 of 5 Species: Pimephales promelas PpKCICR Test Number: 2s 2-Survival and Growth Data Day 750 mg KCVL 900 ma KCIL 1050 ml KCI/L M N O P R S T U V W X 0 to /l t0 / 0 /0 /0 i( /D /0 /0 /0 1 sk qA' S q4. s,( s. 3x f' 6' (0 /0 1 to -1 S S 1 1.2l to S Ib n b o ( k .10_5 _ k, S L A lo 6 ilk~~ 5 .'~ '74 A Pan weight (rmg)t ray eolorcode:i O r! 4.4q jtj.a iqj ' j -g l 1 .0 1 13 -6 ý 3 1 14 -74 1;.-6 4 4 Ab 11~g 4,11 ; J 1 1 ,13 Analyst: -,roLI Date: os "' " -B -Pan + Larvae weight (Mg) 1 .9 t qg ~O.,'o K58 1 i.qs )6.11 l1-.3" WoS6 )bL-t it13q IV443 )-4-6.Analyst: At,".r Date: -o0 -_q C -Larvae weight (mg)=[If-A ,3:bq 5 5.5 .5 " -.3.-00 ,, .Cz. 2 s Weight per initial number ",,o oflarvae (mg) 3' \0 0= C/Initial number of larvae Average Percent weight per reduction initial from control 02... IS 0.,'3 4 5 S &Z O.2T IT.S.number of (%)larvae (mg)Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk = sick, sm. unusually small, Ig unusually large, d&r = decanted and returned, w.= wounded.Calculations and data reviewed: Comments: lage 88 of 107 00 O0"-,4Environmental Testing Solutions, Inc.Pimephalespromelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: PpKCICR#182 Test dates: May 12-19, 2009 Reveawed by: Coacetration(mg/L Rtplicat le Iialrnbcrof FI..alnmbeeof A-Panwaigkt(mgl B-P.,+La-vae Larv-eweigbt(ag) Waghtl/Srvivi.z Meweight/ Co Weigkillaitialaltmber Mea.arvival Mea weight/Initial Pe-1 redscuosfrumn Ka) larvae larv( (dg.ttog) =A-B aumbherflurvaelmg) Sorvivingnualber -of larvae(mg) .(%) .mberof larvae varitoa (%) ootr.l (%)A 10 10 14.77 21.46 6.69 0.669 0.669 Control B 10 10 13.07 20.29 7.21 0.121 0.717 7.0 0.721 100.0 0.317 7.0 Not applicable C lQ 10 14.33 22.18 7.85 0.785 0.785 D 10 10 13.05 19.97 .6.92 0.692 0.692 E 10 10 14.49 21.61 7.12 0.712 0.712 450 F 10 10 14.27 20.79 6.52 0.652 0.686 3.8 0.652 97.5 0.669 67 6.7 G 10 10 13.31 20.29 6.98. 0.698 0.698 H 10 9 14.89 21.03 6.14 0.682 0.614 I 10 10. 13.41 20.82 7.41 0.741 0.741 680 1 10 t0 12.75 19.93 7,18 0.718 0.710 3.6 0.718 K 10 10 15.43 22.26 6.83 0.683 .0.683 100.0 0.710 3.6 1.0_L I0 10 12.93 19.90 6.97 0.697 0.697 1 M 10 8 14.44 19.90 5.46 0.683 0.546 750 N 10 8 14.92 19.87 4.95 0.619 0.495 0 10 8 14.85 20.20 5.35 0.669 0.667 5.1 0.495 80.0 0.533 5.1 256 P 10 8 14.01 19.58 5.57 0.696 0.557 Q 10 6 13.62 17.95 4.33 0.722 0.433 900 R 10

5 13.11 16.11 3.00 .0,600 0.654 7.7 0.300 52.5 0.345 23.6 51.8 S 10 4 14.76 17.32 2.56 0.640 0.256 T 10 6 12.64 16.56 3.92 0.653 0.392 U 10 3 14.23 16.47 2.24 0.747 0.224 V 10 4 14.11 17.39 3.28 0.820 0.733 110 0.328 275 61.7 1050 W 10 4 13.81 16.77 2.96 0.740 0 0.296 37.5 0.7 169 X 10 4 15.13 17.63 2.50 0.625 0.250 1 Dunnett's MSD value: PMSD: 0.0640 8.9 MSD =PMSD Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision.

The PMSD is the sinsimum percent difference between the control and treatment that can be declared statistically sigmficant in a whole effluent toxicity test.Lower PMSD bound determined by USEPA (10th percentile) = 12%.Upper PMSD bound determined by7USEPA (90th percentile) = 30%.Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Intedaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).USEPA. 200 Ia, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-82I-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.Organisms obtained fom Aquatox, Inc.atox05-12-09 0 0-5 0-.1 9:FTS SEnvironmental Testing Solutions, Inc.Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 5/12/2009 Test ID: PpKCICR Sample ID: REF-RefToxicant End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mg/L I 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 0.9000 600 1.0000 1.0000 1.0000 1.0000 750 0.8000 0.8000 0,8000 0.8000 900 0.6000 0.5000 0.4000 0.6000 1050 0.3000 0.4000 0.4000 0.4000 Transform: Arcsin Square Root Rank I-Tailed Number Total Cooc-mg/L Mean N-Mean Mean Min Max -, CV% N Suns Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 450 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 16.00 10.00 1 40 600 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 .A10.00 0 40*750 0.8000 0.8000 1.107.1 1.1071 1.1071 0.000 4 10.00 10.00 8 40*900 0.5250 0.5250 0.8106 0.6847 0.8861- 11.892 4 10.00 10.00 19 40 1050 03750 0.3750 0.6584 0.5796 0.6847 7.979 4 10.00 10.00 25 40 Auxiliary Tests Statistic Critical Skew Kurt .Slapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) 0.81382352 0.884 -1.2632773 2.14787125 Equality of variance cannot be confirmed Hypothesis Test (I-tail, 0.05) NOEC LOEC ChV TU-Steel's Many-One Rank Test 600 750 670.820393 Treatments vs D-Control Maximum Likelihood-Probit Parameter Value SE 95% Fiducial Limits Control Chi-Sq Critical P-value Mu Sigma Iter Slope 8.14828973 1.29114829 5.61763904 10.6789404 0 6.87582293 7.81472778 0.08 2.97727854 0.12272514 4 Intercept TSCR Point ECOI EC05 ECIO ECI5 EC20 EC25 EC40 EC50.EC60 EC75 EC80 EC85 EC90 EC95 EC99-19.259728 3.78378062 -26.675938 -11.843518 Probits 2.674 3.355 3.718 3.964 4.158 4.326 4.747 5.000 5.253 5.674 5.842 6.036 6.282 6.645 7.326 mgL 95% Fiducial Limits 491.787145 380.202898 566.020355 596.229761 500.029379 659.146411 660.693371 577.154041 716.766606 708.079653 634.372816 760.17228 748.153069 682.308654 798.356768 784.335178 724.514185 834.704503 883.459176 829.997827 948.216155 949.026949 890.476916 1035.58115 1019.46098 949.626271 1137.82792 1148.30007 1049.26122 1340.14047 1203.83407 1090.1848 1431.97553-1271.96449 1139.27255 1547.85189 1363.19229 1203.47434 1708.07612 1510.57893 1304.3074 1978.13621 1831,38628 1514.60449 2608.95733 Organisms obtainedfrom Aquatox, Inc.atox05-12-09 )Environmental Testing Solution s, Inc.Statistica1 Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 Test ID: PpKClCR Sample ID: REF-Ref Toxicant End Date:. 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mg/L 1 2 3 4 D-Control 0.6690 0.7210 0.7850 0.6920 450, 0.7120 0.6520 0.6980 0.6140 600 0.7410 0.7180 0.6830 0.6970 750 0.5460 .0.4950 0.5350 0.5570 900 0.4330 0.3000 0.2560 0.3920 1050 0.2240 0.3280 0.2960 0.2500 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7168 1.0000 0.7168 0.6690 0.7850 7.008 4 0.7168 1.0000 450 0.6690- 0.9334 0.6690 0.6140 0.7120 6.687 4 1.627 2.180 0.0640 0.6894 0.9618 600 0.7098 0.9902 0.7098 0.6830 0.7410 3.567 4 0.239 2.180 0.0640 .0.6894 0.9618 750 0.5333 0.7440 0.5333 0.4950 0.5570 5.070 4 0.5333 *0.7440 900 0.3453 0.4817 0.3453 0.2560 0.4330 23.589 4 ..0.3453 0.4817 1050 0.2745 0.3830 0.2745 0.2240 0.3280 16.925 4 0.2745 0.3830 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97055012 0.805 0.28149786 -0.6879762 Bartlett's Test indicates equal variances (p 0.55) 1.1904434 9.2103405 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test -600 >600 0.06396215 0.08923913 0.00265975 0.00172172 0.2649911 2,9 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95% CL(Exp) Skew IC05 IC10 IC15 IC20 IC25 IC40 iC50 608.13 642.56 676.99 711.43 745.86 832.34 889.53 149.72 0.00 651.16 -0.8286 54.75 277.41 687.07 -3.5954 19.21 608.59 724.00 -0.4242 18.63 652.82 760.37 -0.2084 17.03 695.14 791.43 / -0.2232 23.14 784.14 931.75 0.9228 36.16 825.86 1033.39 0.8216 Organisms obtained from Aquatox, Inc.atox05-12-09 I F6 a DaT Page 4 of 5 Species: Pimephales promelas PpKCICR Test Number: "&2.Daily Chemisiry: Day, 0 12 Analyst //IIrt^ L MLr" f. L f-- t'L.h hL Concentration Parameter CONTROL pH ,S.U.) -..6 ", .3 DO3 "mgL-6 75 7t Conductivity 3fa 7i 6__limhos/cm) ' 'r Alkalinity (mg CaCo 3t)Hardness (mg CaCO 3/L)Temperature -k pH (S.U.) "3 ..1 77 "/ _____ 75 -3"1 '4.32-.DO (mg/L) 4. 3 450mg KCIIL Conductivity J 0 1 Temperature 1_gmhos/cm) Temperature DO (mg/L)900 mg KCVL Conductivity (gmrho2/cm) )~7 Temperature1 7.-LN ..PH (s.u .) -__. _____"_- -750 mg KCi/L Conductivity i 1 Temperature .,t. "q pH(S.C) 2q- 3. -27 5 -7 pH (S.U.)-41 -q9 ~ I 4.3a -I-Tb) I SDO (mg/L)1050 mg KCVL Conductivity -(umhos/cm) Temperature (CC)-ntl. 7 -Fia -nt b xn. i q Fin Initial. ia Initial Final. Initial Final STOCK Conductivity _j (mhscm) I Page 92 of 107 a lETS SpEnvcies: Pme.n p T. 5tire p Species: Pimeplhales promelas Page 5 of 5 PpKCICR Test Number: t&L-__Day 3 -4 5 6 Analyst Ii-r Aoda1 L Concentration Parameter CONTROL pH (S.U.) 14it 31~.DO (mg/) .-7.e8 Conductivity Alkalinity (mg CaCO 3 IL) ýHardness 89 (mg CaCO 3/L)Temperature.. (-c) --. A --A.- --.pH (S.U.) J 7.38 to t~q Th7 ._5.."" DO (mg/L) ..1im 450.mg KCI/L Conductivity 1 "z5 i OI5 1 Temperature -LA 4 24. 2'q OAS________ (0 c)_ _ _pH (S.U.) -41 7.'a "i.I *7 , , .L U. " 6 0 0 mg KCV/L Conductivity Temperature .-q .C .-L --I._____ _____ (OC)_pH (S.U.) -.q " . 7 te --/ " " -l 750 mg KC/L DO (mg/L) '} -3 1 -_. -Conductivity I / 9/(pmhos/cm). _' bZ 0 _Temperature( ) .. 2 .1 .. L ." q. "£ " " pH (S.U.) -7,L 4.0.O 7. (P,5 ,7, _ _ ý ' -". b DO (mg/L) -" ."L -, 7-- ". -t4 ,,a 900 mg KCI/L Conductivity Temperature 2 S "24-(0c ) I q .,- V -- T 4 -I -2 .. "4 oH, (S.U.)V, II 7.' 7-CoG Tq.' O -7,01 -'H.q I 4 -4. 'DO (mg/L)1050 mg KCI/L Conductivity (j..mhos/cm) I Temperature (0 C)1n.a Fn naa ni Initial Final Initial Final Initial Fnl Initial Fi l STOCK 1 Conductivity I (jImhos/cm) toe 93 of 107 0EnvironmentalITesting Solutions, Inc.Ceriodaphnia dubia Chronic Reference Toxicant Control Chart 1.14 I I I I I I S t n r I I I I I I I I-[ USEPA Control Limits*(J= 2 Standard Deviations) I I I I 1.12 -1.10 1.08 1.06 1.04 '1.02 I I I I II I I I I I I I I I I I I I 95, 25 Q Cu I-2.0 1.5 1.0 USEPA Warning and Control Limits (7 5 th and 9 0th Percentile CVs).....I. ... .........0.5" 1.4 1.3 1.2 1.1 I I !I I I ! i " I I I I I I I I I I Laboratory Warning and Control Limits (10/h and 2 5 th Percentile CVs)... ... .. .. ... .... .. ..... ... ... ....I I I I I I I I I I I I I I I ! I I I I 1.0 " 0.9 0.8 z% %% Q's "% o Z% z% z% Z-- z% ".. zs ".. ý 'e- 6'V'q a"".) 'aq "q 'p QNO QN0 Q;O, 0", o"', zs* q6ý6'- 00, "0' losl- ý01 "N-Z' zip 03 q0"'Test date 7-day IC 2 5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean IC 2 5)Warning Limits (mean IC 2 5 + SA10 or SA.75)Control Limits (mean IC 2 5 S A.25' SA-90, or 2 Standard Deviations) P age 94 of 4 07 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart" Envir( nmntal Testing Solutions, Inc.State and USEPA Laboratory Laboratory USEPA USEPA Test number Tt date 7-day IC, CT S Control Limits SAle Warning Limits SA-25 Control Limits SA.s 5 Warning Limits SA-90 Control Limits CV (g/L NaCI) (g/L NaCl) CT-2S CTl2S CT -SAJO CT + SnA- CTr-s .CT + S&2 5 CT -SA.SS C" -SA.7s CT -S,, CT + S ,.1 01-08-08 1.09 2 01-08-08 1.09 1.09 0.00 1.09 1.09 0.09 1.00 1.18 0.19 0.90 1.28 0.49 0.60 1.58 0.68 0.41 1.77 0.00 3 0.05-08 1.07 1.08 0.01 1.06 1.11 0.09 1.00 1.17 0:18 0.90 1.27 0.49 0.60 1.57 0.67 0.41 1.76 0.01 4 0104-08 1.07 1.08 0.01 1.06 1.10 0.09 0.99 1.17 -0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.01 5 04-08-08 1.07 , -1.08 0.01 1.06 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.49 0.59 1.56 0.67 0.41 1.75 0.01 6 06-08 106 1.08 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 .0.01 7 .03-08 1.11 1.08 0.02 1.05 1.11 0.09 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.02 8 0708-08 1.07 1.08 0.02 1.05 1.11 009 0.99 1.16 0.18 0.89 1.26 0.49 0.59 1.56 0.67 0.41 1.75 0.01 9 0 5-08 1.09 1.08 0.02 1.05 1.11 0.09 0.99 1.17 0.18 0ý90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.01 O 0 09-08 1.10. .08 002 1.05 1.11 0.09 1.00 1.17 0.18 090 127 0.49 0.59 1.57 0.67 0.41 1.75 0.01 I 1 1 07-08 1.09 1.08 002 1.05 1.11 0.09 1.00 1.17 0.18 0.90 1.27 0.49 0.60 1.57 0.67 0.41 .1.75 0.01 12 11 04-08 1.07 1.08 0.01 1.05 1.11 0.09 1.00 1.17 0.18 0.90 1.27 0.49 0.59 1.57 0.67 0.41 1.75 0.01 13 11 04-08 1.10 1.08 0.01 1.05 1.11 0.09 1.00 1.17 0.18 0.90 1.27 0.49 0.60 1.57 0.67 0.41 1.75 0.01 14 14,-02.08 1.106 1.08 0.02 1.05 1.11 0.09 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.01 15 01.06-09 1.05 1.08 0.02 1.05 1.11 0.09 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 002 16 02-03-09 1.05 1.08 0.02 1.04 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.75 0.02 17 0224-09 1.07 1.08 0.02 1.04 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.02-18 03-10-09 1.07 1.08 0.02 1.04 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.02 19 04-14-09 1.09 108 002 1.04 1.11 .0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.75 0.02 20 05205-09 1.07 1.08 0.02 1.04 1.11 0.09 0.99 .1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.02 Note. 7 d IC2 5 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC 2 5).S = Standard deviation of the IC2, values.Lab ratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC1 5 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.SA I = Standard deviation corresponding to the I 0 w percentile CV. (SA O = 0.08)= Standard deviation corresponding to the 25'h percentile CV. (SA2S =0.17)USE A Control and Warning Limits SA.sS = Standard deviation corresponding to the 75" percentile CV. (SA.75 = 0.45)SA.A9 = Standard deviation corresponding to the 900' percentile CV. (SA.m = 0.62)CV = Coefficient of variation of the IC2, values.USEPA. 2000 Und rstanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.05-05-09 Precision of Endpoint Measurements Ceriodaphnia dubia Chronic Reference Toxicant Data') Environmental Testing Solutions, Inc.Test number Control Control Mean T Survival Reproduction (%), (offspring/female) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 01-08-08 01-08-08 02-05-08 03-04-08 04-08-08 05-06-08 06-03-08 07-08-08 08-05-08 09-09-08 10-07-08 11-04-08 11-04-08 12-02-08 01-06-09 02-03-09 02-24-09 03-10-09 04-14-09 05-05-09 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 30.3 31.8 31.3 31.5 29.6 32.1 30.5 30.9 29.4 28.3 32.4 32.3 31.5 31.5 33.2 31.4 32.2 31.9 33.9 33.6 CT for Control Mean Reproduction (offspring/female) 31.1 31.1 31.2.30.9 31.1 31'0 31.0 30.8 30.6 30.7 30.9 30.9 31.0 31.1 31.1 31.2 31.2 31.4 31.5 CV CT for Control (%) Reproduction CV (%)MSD 'PMSD (%) for PMSD (%)CT 6.6 4.9 6.4 8.0 7.7 6.3 7.9 6.7 7.9 8.5 7.2 5.1 6.2 4.6 4.0 6.6 6.0 5.8 6.7 4.3 5.7 6.0 6.5 6.7 6.6 6.8 6.8 6.9 7.1 7.1 6.9 6.9 6.7 6.5 6.5 6.5 6.5 6.5 6.4 2.2 2.4 2.8.2.7 2.6 2.5 3.0 2.4 2.2 3.0 2.7 2.2 2.4 2.5 2.0 1.9 1.8 2.6 2.0 2.3 7.3 7.7 9.1 8.5 8.8 7.9 9.9 7.7 7.6 10.5 8.4 6.8 7.7 8.0 6.1 6.0 5.7 8.2 5.8 6.8 7.5 8.0 8.1 8.3 8.2 8.4 8.4 8.3 8.5 8.5 8.3 8.3 8.3 8.1 8.0 7.9 7.9 7.8 7.7 Note: CV Coefficient of variation for control reproduction. Lower. CV bound determined by USEPA (1 0 h percentile) = 8.9%.th Upper CV bound determined by USEPA (90 percentile) = 42%MSD =.Minimum Significant Difference PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.Lower PMSD bound determined by USEPA (I 0 th percentile) = 13%.Upper PMSD bound determined by USEPA (90th percentile) 47%.CT =.Central Tendancy (Mean Control Reproduction, CV, or PMSD)(USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.P 05-05-09 Page 96 of 107 ""ni')Environmental Testing Solutions, Inc.Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements 40-. I I I .I I I I I I I I I I I I I I I I I -35~3025 S 520 U 15...................................!Minimum Acceptance Criteria (> 15.0 offspring per surviving female)S I I I I I I ) I I I I I I~40~30~20 Q10 42---I I I I I I I I I ----North Carolina Acceptance Limit (< 40.0%)Kentucky Acceptance Limit (< 30.0%)** I I I ~ I I I I I I I I I I I I I I I I C,'50 40 30 20 10 A'T -I I I I I I I I I I I I I I I I I USEPA Upper PMSD Bound (90°" percentile < 47.0%)-I .1. I I I I I I .... I .. _Ll Test date__:____Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PNISDI )PMSD-"iz~ennm-saigcn~w differe~nce-between the con-tro and-treatmemnt-htcnb declared statistically significant. -- Central Tendency (mean Control Reproduction, CV, or PMSD)ill ige 97 f 1 07Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations) F an. Tý hn L-Page 1 of 6 Sodium Chloride Chronic Reference Toxicant-Test (EPA-821-R-02-013 Method 1002.0)Species: Ceriodaphnia dubia CdNaCLCR #: 80t Dilution preparation in ormation: Comments: NaCI Stock INSS number: I IWs 4 '4 o Stock preparation: t00 g NaCI/I (dissolve 50 g NaCI in 500 ml deionized water)Dilution prep (m ) 600 800 1000 1200- 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume mL) 1491 1488 1485 1482 1479_Total volume (mL) 1500 1500 1500 1500 1500 Test organism source information: Test information: Organism age: I< 24-hours old Randomizing template color: be.ANG..Date and times organisms were born OS-05-OGI OA.1ks T"O&,6 Incubator number and shelf between: location: Culture board: CA'-U -Replicate number: 1 2 3 4 1 S 6 7 1 9 10 YWT batch: Culture board cu number: -L I 10 o I It. 1 121 12.W Transfer bowl information: pH = 1. 4 SU Temperature = -LS. .7. 'C Selenastrum batch: -Daily renewal information: Day Date Test initiation and feeding, MNHSW Analyst renewal and feeding, or batch used termination, time 0 OS-o04- oA oiok o4-2 T 1 OS'0-00%c 00 1.0o OAk- -R- C?& A 3 _ _ _ OsO __ 01__4 05 001-0 ________ oso- A 6 C5o-I1- CA 0-a n 9- 6L1 Control information: Acceptance criteria Summary of test endpoints: % of Male Adults: )7 <20% 7-day LC 5 0 ) 4 c-Do% Adults having 3 d Broods: 33-& 1 001, > 80% NOEC 00-%Mortality: 5 <20% LOEC 10000 SMean Offsorina./Fernale:G_> f 5.0 offspring/female Ch V Mean OsC3.% CV: 4-. 5 -o< 4U.U '/.IC 2 s I o16t. 2-U -- i Iage 98 of 107 ETS Env,. Mm ~Page 2 of 6 Species: Ceriodaphnia dubia CONTROL CdNaCLCR #: VA_Survival and Reproduction Data Replicate number Day 2 3 4 5 6 7 8 ,9 10 I Young produced 0 0 0_ C) 0 0___ 0 C)Adult mortality t __ __j _.- .__- _. \I- t_.2 Young produced D CI c__l C__Adult mortality JL.4 -- k. t ,-- ' '- L._1,,-3 oung produced I 0- (L 7 C 0 0 0 Adult mortality t__ \,- %k 4 Y'oung produced IPk 5 Young produced i- '. \L__ tk -"L % I Adult mortality \- 'I .- \.- \ .-6 Young produced !,... ' [ .[ .L" A Adult mortality 7 jYoung produced j ",j I -'j _ '2. .l j 2 I ____Total young produced 3 lo I olD3li.A 01 ,]OIV 1l.Final Adult M ortality ..._* _... -.__... _.. ..X for 3rd Broods X -C- " -..vote: Adaul mortality (L = live, U aeaoj, 313 = spur brood (single oroou spiu oetween two uays), CU = carry over korispring carried over with adult during transfer). Concentration: % Mortality: Mean Offspring/Female:

33. 6" 600 mg NaCI/L Survival and Reproduction Data Replicate number.Day 1 2 3 4 5 "6 7 8 ' 9 10 I Young produced o 5 3 -c" I Adult mortality k,_.__ L- L. __ ____2_ Yon rdu cedr~l~ JCj) Qj C) CI)IL ~ I~IAdult mortality I _ L 1 U_+ I _ _______ .__ U _ L_ ._ -_3 Yon prdue 1i1 -I I Adult mortality

.. __ _ _ .. ._4 Young produced S I __ k.5 Young produced I' Z ) 'A "l I .Adult mortality \.- __t'_~ -I~-I I'-_~~V~I 6 Young produced C)j lC In--i--- 0 ~____Adult mortality \~_- \ -'-I -~IU U 7 Young produced C) 0 %!S 0 l & IS 0 L (a I (I Total young produced .3q " T5 ý3-1 3 4 J 3S J,4 Final Adult Mortality .-\_-- \ ._ ._.. ._ " ' __,vore .... I A..Il (L -lvU- I1.),_ -S~i nruA .Auu _. t siiug uc ruu _ i. i r.A _:[ .....ei two Udy), Lu f -.... .... over l k0^_.;l corted woiality a L t live, D r anae), B -sprt brood ksingle ood spit between o days), U ý carry over kOisprlng carried over with adult during transfer). .~I % Mortality: 1 (V. 1 i Mean Offspring/Female: 3"t.& I I % Reduction from Control: I- 3.61[age 99 of 107 r Ii I I I I I TS Specie 800 m Page 3 of 6 s: Ceriodaphnia dubia NaCI/L CdNaCLCR #: ___Survival and Reproduction Data 1..... ._ Replicate number Day j 2 3 4 5 6 7 8 9 10 11 Aogrdultmraled L__ J- [_. L_ L_2 Young produced ]J 0 0 C 1 c =)Adult mortality °1J ___Adult mortality IL %,- I .._ k. -% k.-_ _._I Young produced I C- ___ __. 0 c i c 'c .s )Adult mortality C 1 1 V I 4 Young produced __ O _ i0 1 0 I~T 01~ Id Adult mortality L[_ k--_ --I._ -'.. ..7[ Young produced[ kI i s i0 .Adult mortality --. I ...7 Young produced- -: 16 .1t, IS- C S 0 0 Total young produced .3.3 3313$ I .n 32 3' 33 Final Adult Mortality .. ..._ j._.. k- 1, U .Note: Adult mortality (L live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer). w Conceniration: % Mortality:' 07.Mean Offspring/Female: 2Z&. L 1 1000 mg NaCI/L% Reduction from Control: I .7.7. I Survival and Reproduction Data Replicate number Day _2_1_3_1_ _5f 1 2 63 4 5 6 8 9 t10 I Young produced n_____ C) (0 0_ _ _Adult mortality ._ , .. I I... k .__. k J_. _2 Young produced I '- .K)I )' I +-I C) 1 _ _ 0[ Adult mortality I L-[ ----l I Adult mortality, L ._4 -..- ý.. ., .. k _... I.Young produced [ T Adult mortality Iki- ... % IU-f LI L1 5_ Young produced 1~ -_ [ 1 I 1~ k{~~ 1~Adult mortalit 6 Young produced D_ clT0 1J 0- C I) IS__ Adult mortality [L _L U_ =L_~ U J_ -- \_ '--_7 Young produced L, I z IS l. " o- '9 I()3 4)Total young produced [ 30 30 21 3o 3 -Final Adult Mortality U 7..-....=. v T. ' ..__ ._Arw tU I ;I~tI~ =L I:VL -U ) .I J .~iIUUU UUU~L1UL~ILUUy) A WYU~~IL)i~ cried o with oadulty duL. I , tr afe ), _.-- __t brood __i_ e_ brood_ _ _ be_ _twoys), C0U --arry over _ _piIg carried over with adult during transfer). [Concentration! 4.I % Mortality: I oD -7 I p ______I Mean Offspring/Female: 3o.1 I% Reduction from Control: 8.67. 1 tage 100 of 107 I Ern..MT.a.. Page 4 of 6 Species: Ceriodaphnia dubia-1200 mz NaCI/L" CdNaCLCR #: g81 Survival and Reproduction Data Replicate number Day __ 1 2 3 4 5 6 7 8 '9 10 I [ Young produced : C a 0 ) C) C) r) C Adult mortality ..-...2 Young produced ©l 0 0 0 Q 0 1 C) 0 0)IAdult mortality I +,-I I- --I +I 3 Youngproduedl ý ý 0 1 QA c I n I O" Adult mortality L -1 ,, I -* [ v- j L.Adult mortality_ Young produced -I I _Adult mortality"7 Young produced .-s Total young produced Is I 8 1 g i -t l5 Final Adult Mortality L k- 1. _. _ I_._ _ , Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO carry over (offspring carried over with adult during transfer). 065,eAL-4JQ+/-..A- CAILICN O'eAL Concentration: % Mortality: 07 Mean Offspring/Female: I5-4.I 1400 mg NaCI/L% Reduction from Control: -52 ,'/77 Survival and Reproduction Data I_ ._ _Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced ) ) () o C ) 0 Adult mortality _ ._ .2. Yung produced ILQ c___ C___ C___ C___ 1___Ad'2ult mortality I .]__UJLJ1 L-i I_'V&_1 6 Young produced]dC)tm)ral1 0] = 0_1 0 1 0 1 0 1 Adult mortality [L1 _Young produced [ L1 2 (2)"C. ', 5 i Young produced )kC L 6 Young produced C) 4 0 Q 0 01 Adult mortality. I -,-- I \ .- -I -Total young produced ! " 5 -l I 5 3 q Final Adult Mortality [ -' --'- 1 '- '- '_*I~eA'b W-4" Note- Adult mortality (L = live, U = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer). I Conc.l /. lkýrtalitv' I i Mean Offspring/Female: ' -q I% Reduction from Control: -7% .0.rage 101 of 107 W EnJ En, U I Verification of Ceriodaphnia Reproduction Totals Iron on nental Testing Solutions, Inc.C rol D y .Replicate number Total 1 2 3 4 5 6 7 8 .9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 .0.0.0 .0 0 0 0 0 0 0 0 3 0 0 .0 0 0 '0 0 0 0 0 0 4 6 4 5 5 6 5 5 6 4 4 50 5 13 1 13 12 1 12 1 12 1 14 12' 12 13 13 126 6 0 17 0 :0 0 0 16 0 17 15 65 7 17 0 1 17 14 16 16 _ 0 15 00 95 Tat 36 34 34 31 34 I 35 33 33 34 32 336 6, 0 g NaC1IL Da Re plicate number Total 1 2 3 4 5 6 7 8 9 Tt 0 0 0 0 0 0 0 2 0 0 o0 0 0 3 0 _ 0 0 0 0 0 0 0 0 0 4[645 5 5 6 5665 53 S 12 1 13 14 14 14 13 13 1 1 13 13 130 6 1 0 18 0 16 0 0 180 0 0 52 17 0 0 18 15 016 1616 113 To 5 35 34 35 37 34 36 33 35 34 348 8N ng NaClI/L Re plicate number Total 1 2 3 4 .5 6 7 8 9 1 Tt 1 0 0 0 .0 0 0 0 0 ,0 0 0 2 0 0 0 .0 0 0 0 00 0 0 0 0 0 0 0 0 0 0 01 5 4 4 5 5 5 6 5 5 5 49 5. 13 13 I1 13 I1 12 13 .12 12 12 122 0 0o 00 17 0* 16 49 7 16 156 1 15 1 15 00 112 To I 34 33 30 I 33 33 35 36 32 33 33 332* ---a a a a 1000 mg NaCIIL Day Reicate number -Total 1 1 2 3 4 5 6 7 8 9 10 1 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 4 6 5 4 5 5 5 4 5 49 5 13 1 12 10 10 13 11 1112 12 115 6 0 0 0 0 0 17 0 0 0 15 32 7 13 14 12 15 13 0 14 14 16 0 111 Total 32 29 30 30 27 35 30 30 32 32 307 1200 mg NaCnL Re plicate number Total 1 2 3 4 5 6 7 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 5 4 4 5 4 4 4 4 44 5 5 5 7 3 7 5 .5 6 5 5 53 6 0 7-0 0 0 0 0 0 1 0 6 7 7 5 9 6 5 8 6 4 7 5 0 55 Total 15 19 18 12 19 16 13 18 14 15 159.1400 mg.NaCML Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 3 4 2 5 4 3 3 3 2 34 5 4 0 3 3- 5 5 6 0 0 4 30..6 0 4 0 0 0 0 0 2 0 0 .6 7 3 1 0 0 5 2 0 0 0 3 14 Total 12 8 7 5 15 11 9 5 3 9 84 r-4 0 E._U z Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Iro rmental Testing Solutions, Inc.Test number: Test dates: Reveiwed by: CdNaCICR #89 May 05-12, 2009 Concent ation Replicate number Survival Average reproduction Coeffcient of Percent reduction from (mg/L aCI) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) variation (%) control (%)Cont ol- 36 34 34 31 34 35 33 33 34 32 100 33.6 4.3 Not applicable 60( 35 35 34 35 37 34 36 33 35 34 100 34.8 3.3 -3.6 80( 34 33 30 33 33 35 36 32 33 33 100 33.2 4.9 1.2 100 *32 .29 30 30 27 35 30 30 32 32 100 30.7 7.0 8.6 120 15 19 18 12 19 .16 13 18 14 15 100 15.9 15.8 52.7 140E 12 8 7 5 15 11 9 5 3 9 90 8.4 43.2 75.0 Dunnett's I PMSD: 4SD11 value: 2.291 MSD = Minimum Significant Difference 6.8 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.Lower PMSD bound determined by USEPA (10"' percentile) = 13%.Upper PMSD bound determined by USEPA (90"' percentile) = 47%.Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. al Protection Agency, Cincinnati, OH.USEPA. 2,01a, US Environmen 0 0 0-.4 0 F nvlronmentalTesdng Solutions, Inc.Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/5/2009 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 5/12/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: -Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mg/L 1 2 3 4 5 6 7 8 9 10 D-Control 36.000 34.000 34.000 31.000 34.000 35.000 33.000 33.000 34.000 32.000 600 35.000 35.000 34.000 35.000 37.000 34.000 36.000 33.000 35.000 34.000 800 34.000 .33.000 30.000 33.000 33.000 35.000 36.000 32.000 33.000 33.000 1000 32.000 29.000 30.000 30.000 27.000 35.000 30.000 30.000 32.000 32.000 1200 15.000 19.000 18.000 12.000 19.000 16.000 13.000 18.000 14.000 15.000 1400 12.000 8.000 7.000 5.000 15.000 11.000 9.000 5.000 3.000 9.000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 33.600 1.0000 33.600 31.000 36.000 4.255 10 34.200 1.0000 600 34.800 1.0357 34.800 33.000 37.000 3.262 10 129.50 75.00 34.200 1.0000 800 33.200 0.9881 33.200 30.000 36.000 4.877 10 95.50 75.00 33.200 0.9708*1000 30.700 0.9137 30.700 27.000 35.000 7.045 10 68.00 75.00 30.700 0.8977*1200 15.900 0.4732 15.900 12.000 19.000 15.814 10 55.00 75.00 15.900 0.4649*1400 8.400 0.2500 8.400 3.000 15.000 43.179 10 55.00 75.00 8.400 0.2456 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.81542361 1.035 0.21736896 0.95569016 Bartlett's Test indicates unequal variances (p = 8.87E-03) 15.3749275 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 800 1000 894.427191 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95% CL Skew2Stt 42 6664f'107 Olt 19821A Al 44 IC10 IC 15 IC20 IC25 1C40 IC50 993.6 29.0004806 921.551731 1015.99639 " -0.8830 1022.02703 8.689644 1004.45361 1037.76034 -0.3344, 1045.13514 7.64509171 1029.66986-1060.12003 -0.0590 1068.24324 .7.05805813 1055.32988 1081.38741 0.0234 1137.56757 7.32664661 1123.52221 1151.44087 0.2385 1183.78378 9.03530003 1167.78076 1201.21731 0.2331 05-05-09 S'9 S Statistical Analyses Environmental Testing Solutions, Inc.Used for PMSD calculation only. Ceriodaphnia Survival and Reproduction Test-Reproduction Stant Date: 5/5/2009 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant Enc Date: 5/12/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sar ple Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Co ments: Cenc-mg/L I 2 .3 4 5 6 7 8 9 10-Control 36.000 34.000 34.000 3 1.000 4.000 35.000 33.000 33.000 34.000 32.000 600 35.000 35.000 34.000 35.000 37.000 34.000 36.000 33.000 35.000 34.000 800 34.000 33.000 30.000 33.000 33.000 35.000 36.000 32.000 33.000 33.000 1000 32.000 29.000 30.000 30.000 27.000 35.000 30.000 30.000 32.000 32.000 1200 15.000 19.000 18.000 12.000 19.000 16.000 13.000 18.000 14.000 15.000 1400 12.000 8.000 7.000 5.000 15.000 11.000 9.000 5.000 3.000 9.000 Transform: Untransformed 1-Tailed C nc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD-Control 33,600 1.0000 33.600 31.000 36.000 4.255 10 600 34.800 1.0357 34.800 33.000 37.000 3.262 10 4.198 2.287 2.291 800 33.200 0.9881 33.200 30.000 36.000 4.877 10 0.399 2.287 2.291*1000 30.700 0.9137 30.700 27.000 35.000 7.045 10 2.895 2.287 2.291*1200 15,900 0.4732 .15.900 12.000 19.000 15.814 10 17.667 2.287 2.291*1400 8.400 0.2500 8.400 3.000 15.000 43.179 10 25.153 2.287 2.291 Au iliary Tests Statistic Critical Skew Kurt Kol ogorov D Test indicates normal distribution (p > 0.01) 0.81542361 1.035 0.21736896 0.95569016 Ba lett's Test indicates unequal variances (p = 8.87E-03) 15.3749275 15.0862722 Hy othesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dujnett's Test 800 1000 894.427191 2.29089729 0.06818147 1241.68 5.01851852 6.4E-36 5,54 Treatments vs D-Control 05-05-09

I Page 5 of 6/Species: Ceriodaphnia dubia CdNaCLCR#:

" Daily Chemistry: Day 0 .1 2 -S .Analyst f Lht-, PtAL%,-Concentration Parameter I SpH (S.U.) --T" H DO( m1L) ; , q ' .CONTROL Conductivity 3 //(inmhos/cm) Alkalinity (mg CaCO3/L) 6 7- otq Hardness (mg CaCO 3/L)Tem perature -LA 1_ IL pH (S.U.) '.55____ __.,___ "-_ _" DO (mg/L) 1'7 7A 600 mg NaCI/L Conductivity (ýIhos/cm) Temperature (0c )j_ _ .o D .A 1 4 ._ _ -J 4. 1 c ', S.P H (S .U .) t a -., , 7 ,5 "72 T 7.5S ,S 0 .r. " S -A DO (img/L) -, --. --800 mg NaCI/L Conductivity (iimhos/cm) Temperature I 2L (0 c) q_. 0."__ .______ _ ._ _ 2_ .______.pH (S.U.) -_.__ 7 ]'3 :57- *1-2 1.5L1 -4 'DO (rag/L) "-4,q 1l -7q q -4 .1000 mg NaCVL Conductivity (piihos/cm) 0 Temperature z 9 (0c) ZI.9 q. qq c _~ t~pH (S.U.) _ -_6 .--4. "7 g-._ " __.___DO (rm/) "-. "-1200 mgNaCImL Conductivity -5l (pmhos/cm)p Temperature

2. ~9 2. L.pH (S.U.)i31k H116 -315 *1~ TO 7.578 11m.DO (mg/L)1400 mg NaCI/L Conductivity (mhos/cm)Temperature (0C.Initial Final Initial Final Initial Final STOCK I Conductivity I (4mhos/cm) age 106 of 107 IlE TS Page 6 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: Day6 3 4 56__ __ __ __Analyst tALt" LA-6 I LN ~Arr Concentration Parameter IpH (S.U.) .13 1 t 7.O DO (mg/L) T1~73o iO .~ I~-CONTROL Conductivity 32- z (ýtmhos/cm)

Alkalinity (mg CaCO3/L)' 6 1 Hardness (mg CaCO 3/L) (Temperature 7AS -.." .4 l.S...(0c)p (S.U.) 3 ".7, .-.DO 1./L 8.0 1159.0.1 600 mg NaCVL Conductivity

q ([mhos/cm)

Temperature VA -I ..*pH(0 C) ..-_.- _,__ ---800 mg NaCI/L Conductivity BI 0 ( )Temperature " -".*O "t -,.s "L .' .\ "q L 0 pH (S.U.) *%.0 1.50 1.47 __,___ _____. _, ____.1000mgNaCI/L Conductivity I l/l ~ l (pmhos/cm) Temperature ILA "t4* " PH (°C) 1-60 .50 1. *S.O DO 73/L 4,1-93 12000 mg NaCL/L Conductivity 0 L (pmhos/cm) Temperature Is.s~ ri. VA t'-i RS__________ _ pH___ ( S.C ) _______1_ ________ --I -DH (S.U.)-4-63 17 ;:; 7.,l .III q,'fl -4,2 "1 "I, q4 1 T I DO (mz/L)*1400 mg NaCIL.Conductivity I Temperature (0c) *-2B-3. -5 ., .1? "" 0-'. 3. OCK --fn ----------

mho c e 107 of 107 Initial 11 Final I Initial. Final -Initial I Initial Final PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000---j~iNTEROFFICE SB-2A-S QN)SODDY-DAISY .-TN37384 Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 TN0026450 103 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND MrlNIT0PRINF PFR11io I EFFLUENT From YEA .T MO DA FromI1 09 105 1011 To 1o91o5 31ýATTN: Stephanie.A. Howard*** NO DISCHARGE NOTE: Read instructions before completinq this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH SAMPLE 7.6 ........ 8.7 12 0 20/31 GRAB MEASUREMENT ý00400 1 0 0 , PERMIT'~ ~ ~ ~ *,- *~ r : SU ~ THREE,< GRPA B REQUIREMENT

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.........!EFFLUENT GROSS VALUE , , 4-L MAXIMUM WEE K' ********-1 115 831 GA iSOLIDS, TOTAL SUSPENDED SAMPLE 177 249 26 19 MEASUREMENT .1 00530 1 0 0 *6 1250 LBSDY 3 100 MG/L VWEEKLY 3 GRAB EFFLUENT GROSS VALUE .MO AVG~ -DAILY Mx MO AV DAILY MX OIL AND GREASE SAMPLE <84 <122 26 < 19 0 1 GAB MEASUREMENT2 00556 1 0 0 ~ 2PERMITI 1~90 25LBS/DY F1542 MG/L IEKY GRAB EFFLUENT GRSSVLUIREMO r IM2VG ,EFFLUENT GROSS VALUE M. AG .DAILY MX MO AVG MX FLOW, IN CONDUIT OR THRU SAMPLE 1.251 2.488 03 ...0 31 / 31 TOTALZ TREATMENT PLANT. MEASUREMENT .50050 1. 0 0 PERMIT MGD SEE TOTALZ REPORT REPORT EFFLUENT GROSS VALUE REAUIREMENT E T MA'MO AV SAMPLE MEASUREMENT PE~RMIT << r~~i SAMPLE MEASUREMENT SAMPLE MEASUREMENT PERMIT -'h REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel T E E Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engfneer information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 09 06 09 Sequoyah Site.Vice President accurate, and complete. I am aware that there are significant penalties for submitting false .SIGNATURE OF PRINCIPAL EXECUTIVE information, includingthe possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)The Turbine Building Sump (TBS) was discharged directly to the Yard Pond (YP) on 5/5/09 @ 0850 -5/9/09 @ 1425 and on 5/11/09 @ 1625 -5/11/09 @ 1740.EPA Form 3320-1 (REV 3/99) -Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different).Name TVA -SEQUOYAH NUCLEAR PLANT Address P.0. BOX 2000... -.JINTEROFFICE SB-2A-SQNL-SODDY -DAISY TN 37384 Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)(SUBR 01)TN0026450 167 F F-FINAL PERMIT NUMBER ýDSCARGE NUBR METAL CLEANING WASTE POND Form Approved, OMB No. 2040-0004 flAM ITn DlIKlt DC:DInMr I EFFLUENT! . I

I YEAR I MO DAY I MO DA From 0 09 1 05 01 1 To 1 09 1 05 1 31 I ATTN: Stephanie A. Howard NO DISCHARGE

@ ...NOTE: Read instructions before completing this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE MEASUREMENT -* 12 100400 1 0 0 PERMITh 6 , ***~~~~****<~~.0;

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--~ -.10 , MGIL .., DAILY. COMPOS EFFLUENT GROSS VALUEREUEMT

& 'ALy X FLOW, IN CONDUIT OR THRU SAMPLE * ********ITREATMENT PLANT MEASUREMENT 03 **50050 1 0 0 FERMIT .... ... .R:8EQUIREMýN MNIG DI Y c-LCI EFFILUENT GROSS VALUE.E RUIREMNT MO AVGý -DAILY MX NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE.direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted, Based on my inquiry of the person or persons who manage the system, or those persons directly rebponsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 09 06 09 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)No Discharge this Period EPA Form 3320-1 (REV 3199) .Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000---A1INTEROFFICE SB-2A-SON. .SODDY -DAISY TN 37384 Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)(SUBR 01)TN0026450 110 G F-FINAL PERMIT NUMBER 6DISCHARGE NUMBER] RECYCLED COOLING WATER Form Approved.OMB No. 2040-0004 7 EFFLUENT MONITORING PERIOD I R DAY .. NO DISCHARGE XX From L09 I 001 To l0i I INOTE I I. reau I I IUn.st IUIIo UeoIUe comUpIeInJIgLII tsfo rm.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.EX FREQUENCY SAMPLE OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS I I I I TEMPERATURE, WATER DEG.!CENTIGRADE 00010 z 0 0 INSTREAM MONITORING SAMPLE MEASUREMENT 1PER MIT REQUIREMrENT~ 04 DEG C 04 DEG C 38.3 DAILY MX IJýRy 01-PAR-4 PH 00400 1 0 *0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDEI 00530 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 00556 1 0 0 IEFFLUENT GROSS VALUE.FLOW, IN CONDUIT OR THR TREATMENT PLANT 50050 1 0 0 EFFLUENT GROSS VALUE D SAMPLE MEASUREMENT ,IrW 12-PERMiT ~ ** *w;~'REQUIRHEMENýT ~6.0 M I9.0 M SU WIEEKLY GRAB D I *~'~'MAXIMUM SAMPLE MEASUREMENT

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~-4~J-NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my I --direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the-system, or those persons directly responsible for gathering the Principal Enviro Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PR!information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AU nmental Engineer INCIPAL EXECUTIVE THORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)No Discharge this Period EPA Form 3320-1 IREV3/991 Previous editions may be used Page 1 of 1 EPA Form 3320-1 (REv'3/99) Previous editions may be used Page 1 of 1 PERMITTEE*NAME/ADDRESS (Include Facility Name/Location if Different) Name .TVA -SEQUOYAH NUCLEAR PLANT Address P.Oi BOX 2000-INTEROFFICE SB-2A-SQN. SODDY -DAISY TN 37384 Facilitv TVA -SEQUOYAH NUCLEAR PLANT*Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)'(SUBR 01 )TN0026450 L 110 T F F-FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Form Approved.OMB No. 2040-0004 RAN llITnlPIf'NC PF: RIfn I EFFLUENT YEAR MO DY YEAR MO DAY N From l 1 1 *** NO DISCHARGE* NOTE. Read instructions belore completing this form.rI ...... -FEUnIY SML PARAMETER IC25 STATRE 7DAY CHRý,CERIODAPHNIA TRP3B 1 0 0 EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.EX FRQUENCY1 SAMPLE OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS I I- I H H t r T 23 PERMIT REQUIREMENT ~45.2 MINIMUM.PERCENT SEMI, ANNUAL COMPOS~+/- 4 4 ~ H............~. f<.-- H H t r T SAMPLE MEASUREMENT 23~PERMIT~REQUIREMENT ~.,********~ ~45.2 ~PERCENT SEýMC COMPOS ANUAL SAMPLE MEASUREMENT IREQUIREIMENT I SAMPLE MEASUREMENT REQUIREMENT ~H SAMPLE MEASUREMENT -ýERMFIF SAMPLE MEASUREMENT PERMIT REQUIREMENT -I --- -- -.-I_ _ -. , 4- __ __ __ _ -4 ___ __ .--H--- -- r. .. ..SAMPLE MEASUREMENT PEIRMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my .-direction or supervision in accordance with a system designed to assure'that qualified personnel* Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVI TYPED__ __PRINTED_ information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000.-ANTEROFFICE SB-2A-SQ .N. .SODDY -DAISY TN 37384TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)(SUBR 01)TN0026450 F-116 G ] F-FINAL PERMIT NUMBER ýDISCHARGE"NMBER BACKWASH Form Approved.OMB No. 2040-0004 i " -k MONITORING, PFRII)D i EFFLUENT I YEAR 7 MO I DAY YEAR MO DAY NO DISCHARGE From T 09 05 01 T T 0 05 31O NOTE: Read instructions before completing this form.der penalty, of law that his docurt ant and all attachments were prepared under my I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER F Timthy .Clarydirect ion or Timohy .Claryproperly ga persons wh information Sequoyah'Site Vice President accurate, a information TYPED OR PRINTED der penalty of law that this documnent and all attachments were prepared under my r supervision in accordance with a system designed to assure that qualified personnel ather and evaluate the information submitted. Based on my inquiry of the person or 0o manage the system, or those persons directly responsible for gathering the the information submitted is, to the best of my knowledge and belief, true, nd complele. I am aware that there are significant penalties for submitting false , including the possibility of fine and imprisonment for knowing violations. Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT L ________________________________________________ L ____________________________ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)Operations performs visual inspections for floating debris and oil and grease during all backwashes. E~M rurrri 3JZU-I fr¶~V or~O~ f-re vtous eoirrons may oe useo Page 1 of 1 EPA Form 3320-1 (REV 3/99)P~revious editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA -SEQUOYAH NUCLEAR PLANT Address PO. BOX 2000.- INTEROFFICE SB-2A-SON) .. .. ..SODDY -DAISY TN 37384 Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) M " (SUBR 01)TN°026450 117 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER BACKWASH MONITORING PERIOD EFFLUENT Form Approved.OMB No. 2040-0004 YEAR MO DAY YEAR MO DAY From 09 105 101 TO 09 105 131 NO DISCHARGE = ...NOTE: Read instructions before completing this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF. TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE ** 0 9A 0 1 / 31 VISUAL MEASUREMENT 01345 1 0 10 ~ ~PERMIT >, .**4~ <REfPORT, PASS=0 $EE VISUAL EFFLUENT GROSS VALUE ' FAIL=1 PERMIT h.__ _ _ _ _ _I_ ___ MO TOTAL OIL AND GREASE VISUAL SAMPLE 0 94 0 1/31 VISUAL MEASUREMENT 0"4 **ý84066 1 0 0 PEMT REOT YS1~~-W SEE >VISUAL REUIREMENT'I-PRjf EFFLUENT GROSS VALUE , REQ.MO TOTAL NPERMIT=O < ..SAMPLE MEASUREMENT PERMIT j..REQUIREMENT

.;, SAMPLE MEASUREMENT-rPERMITJ-REQUIREMENT

< <4j SAMPLE MEASUREMENT ...PERMIT<>"I REQUIREMENT' SAMPLE MEASUREMENT SAMPLE MEASUREMENT PERMIT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cenify under penalty of law that this document and all attachments were prepared under my -, I l -TELEPHONE DATE.direction or supervision in accordance with a system designed to assure that qualified personnel 'i J. j "" -6 Timothy P. Cleary properly gather and evaluate the information submitted Based on myinquiry of the person or persons who manage the system, or those persons directly responsible for gathering the .Principa Environmenta Engineer information, the information submitted is, 1o the best of my knowledge and belief, true, 423_843-6700_09 06 09 Sequoyah Site Vice President accurate, and compele. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE-__information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED i .CODE.COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000-.- INTEROFFICE SB-2A-SQN. .SODDY -DAISY TN 37384 Facilit TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.DISCHARGE MONITORING REPORT (DMR)(SUBR 01) 0MB No. 2040-0004 TN0026450 118 G F -FINAL PERMIT NUMBER DISCHARGE NUMBER] WASTEWATER & STORM WATER MONITORING PERIOD I EFFLUENT YEARI MO I DAY YEARI MO 1I DAY From 09 05 01 To 09 1 05 T31 NO DISCHARGE XX NOTES Read instructions before completing this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE. , 19 MEASUREMENT 19 00300 1 0 D PERMIT M.G-:L :-MI***- --, * -MGIL _ 'TWICE/ GRAB: EFFLUENT GROSS VALUE WEERMETEK< ~ ~ ~ :~'-- --- WK ~DAILY MN~ ~WE~SOLIDS, TOTAL SUSPENDED SAMPLE ** **9 MEASUREMENT 100530 1 0 0 1 PERMIT **" .* .MG/L TW/NICE/I GRAB EFFLUENT GROSS VALUE DAILY MXMENT -WEEK<iSOLIDS, SETTLEABLE SAMPLE **** *25 MEASUREMENT 25 00545 1 0 0ý PERMIT>K " *** ~ ~ >>?****~- 1.0'- MUL -ONCE/ >>GRAB~EFFLUENT GROSS VALUE R M>>DAILY MXV'K 'K' MONTH FLOW, IN CONDUIT OR THRU SAMPLE **TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 RPERMIT ET REPoT MGD ONCE/ ESTIMA SAMPLE MEASUREMENT REQUIREMENT I SAMPLE MEASUREMENT REQUIREMENT ->'KKP SAMPLE MEASUREMENT

~ ~ <PERMIT<>, ,--'- P----- ~ v REUIrý N.---.'I NAME/TITLE PRINCIPAL EXECUTI\Timothy P. Cleary Sequoyah Site Vice Presi TYPED OR PRINTED fE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, dent accurate, and complete. I am aware that there are significant penalties for submitting false information. including the possibility of fine and imprisonment for knowing violations. Principal Environmental Engineer TELEPHONE DATE I 09 06 0 09 423 843-6700 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER CODE YEAR{ MO DAY I COMMENTS AND EXPLANATION OF ANY VIOLATIONS. (Reference all attachments here).During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of I}}

ML091800413

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Dear Mr. Patrick Cromer:

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ML091800413

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Dear Mr. Patrick Cromer:

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