ML091800413
| ML091800413 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 06/11/2009 |
| From: | Cleary T Tennessee Valley Authority |
| To: | Cromer P Office of Nuclear Reactor Regulation, State of TN, Dept of Environmental & Conservation, Div of Water Supply |
| References | |
| Download: ML091800413 (121) | |
Text
{{#Wiki_filter:j Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 June 11, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR MAY 2009 Enclosed is the May 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. Sincerely Timothy P. leary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR." Form Approved. -Narn---- W TVA-- SEQUOYAH-NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR). Address P.O BOX 2000 (SUBR 01 ) OMB No. 2040-0004
. . ..- .i..NTEROFFICE SB-2A-SQN. TN0026450 101 G -FINAL SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT PERMIT NUMBER IDISCHARGE NUMBER DIFFUSER DISCHARGE Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT YEAR MO DAY YEAR MO I DAY NO DISCHARGE = ...
ATTN: Stephanie A. Howard From 09 05 01 To[ 09 05 31 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
~ANALYSIS EX OP TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE
- 23.8 04 o 31 /31 MODELD CENTIGRADE MEASUREMENT 00010 Z .0 0 PEMIT
- 0. . D5 EG. C. SEE :K REQ INSTREAM MONITORING .. -& .<"-DAILYMX- PERMIT TEMPERATURE, WATER DEG. SAMPLE * ** 40 0 04 0 31 I 31 RCORDR CENTIGRADE MEASUREMENT
ý00010 1 0 0 .PERMIITrt >>~t REPORT~* DEG. C. SE ~CK FEQ: JEFFLUENT GROSS VALUE REQUIREMENT. ALYM :RMIT PE'~ jA.:V~ ITEMP. DIFF. BETWEEN SAMP. & SAMPLE . 2.1 04 0 31/31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 S 0 3 0ERMI DEG. C.
- CNTI NCALTD EFFLUENT GROSS VALUE ~ REQ~iIREMENT1 .v# .< DAILY MX% UOUS~
PH SAMPLE 7.5 7.9 MEASUREMENT 12 0 7/31 GRAB 00400 1 0 0 P. RMIT: .*.***** -E ... 60 9.0 SU WEEKLY GR... EFFLUENT GROSS VALUE MINIMEM .MAIMUM 19 " SOLIDS,TOTAL SUSPENDED SAMPLE
- 13 13 19 0 1 / 31 GRAB MEASUREMENT 00530 1 0 0 ,.. !PERMIT ." .,-
Jfl*, EFLUNTGOS VLUREQUIREMENT 3 100MGL MNHLYGRB _________ROSSVALU MO AVG. %DAILY MX OIL AND GREASE SAMPLE <5 MEASUREMENT
** <5 j9 0 1 / 31 GRAB 00556 1 0 0i REQU~IREM.ENT MON EFFLUENT GROSS VALUE r
- MOAVG M AV .IL X
- AILYEMX FLOW, IN CONDUIT OR THRU SAMPLE *1452 03 ** /31 RCORDR TREATMENT PLANT MEASUREMENT .
50050 1 0 0 RI*E'GPPEME - " MGD i****" -.
.. ONTIN N O 13*
IEFFLUENT GROSS VALUE DAILY MVX :.< PdUQUS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel TELEPHONE DATE Timothy P. Cleary xoperly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system,.or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true,. Principal Environmental Engineer Sequoyah Site Vice President 423 843-6700 09 06 09 accurate, and complete. I am aware that there are significant penalties for submitting false SIG NATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED )FFICER OR AUTHORIZED AGENT. AREA I NUMBER YEAR MO I DAY CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: 1. CCW data 2. Veliger monitoring data EPA Form 3320-1 (REV 3/99) Previous editions may'be used Page 1 of 2
DMR Attachment CCW Data CCW TRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 05/13/2009 @ 1155 0.22 mg/I 05/14/2009 @ 1644 CLS TN EPA 8015 CCW CHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 05/13/2009 @ 1200 <0.10 mg/I 05/14/09 @ 1655 CLS TN EPA 8015 ____________ I________________ i_____________ 1* I__________
Mean#ofMean# of NOTES: % Sample Date ZM/m3 % Settlers Water TempSample Date Asiatic r Temp. OCATION SUB, Gravid Asiatic COLLECTED BY
.Mm ( SC) Clams/m3 (C) LOCATION Clam 11121/2008 a 0 12 11/16/2008 230 12 Inplant RCW Dick Adcock 11/24/2008 31 0 11 11/24/2008 138 11 Inplant. RCW Dick Adcock 12/4/2008 0 0 10 12/04/2008 15 10 Inplant .RCW Dick Adcock 12/8/2008 0 0 9 12/08/2008 0 9 Inplant RCW Dick Adcock 12/15/2008 0 0 9 12/15/2008 0 9 Inplant RCW Dick Adcock 12/22/2008 0 0 9 12/22/2008 0 9 Inplant RCW Dick Adcock 12/29/2008 0 0 9 12/29/2008 0 9 Inplant RCW Dick Adcock 01/09/2009 0 0 8 01/09/2009 0 8 Inplant RCW Dick Adcock 01/16/2009 0 0 7 01/16/2009 0 7 Inplant RCW Adcock / Bryant 01/22/2009 0 0 7 01/22/2009 0 7 Inplant RCW Adcock / Bryant 01/30/2009 0 0 9 01/30/2009 0 9 Inplant RCW Adcock / Bryant 02/06/2009 0 0 6 02/06/2009 0 6 Inplant RCW Adcock / Bryant 02/13/2009 0 0 11 02/13/2009 0 11 Inplant RCW Love 02/20/2009 0 0- 9 02/20/2009 0 9 Inplant RCW Bryant 02/26/2009 0 0 9 02/26/2009 0 9 Inplant RCW Bryant 03/06/2009 0 0 8 03/06/2009 0 8 Inplant RCW Dick Adcock 03/13/2009 0 0 10 03/13/2009 0 10 Inplant RCW Dick Adcock 03/27/2009 0 0 14 03/27/2009 0 14 -Inplant RCW Wayne Wright 03/30/2009 0 0 13 03/30/2009 0 13 Inplant RCW Dick Adcock 04/09/2009 28 .0 14 04/09/2009 0 14 Inplant RCW Dick Adcock 05/06/2009 880 24 19 05/06/2009 21 19. Inplant RCW CNW 05/12/2009 15091 0.3 20 05/12/2009 47 20 Inplant RCW CNW 05/19/2009 3961 2 20 05/19/20 09 .445 20 Inplant RCW BJ 05/27/2009 7696 0.8 22 05/27/2009 457 22 Inplant RCW CNW
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Address P.O. BOX 2000 (INTEROFFICE Sa-2A-SQNk TN0026450 101 G F - FINAL SODDY - DAISY TN 37384 PERMIT NUMBER " DISCHARGE NUMBERI DIFFUSER DISCHARGE Facilitv TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY I MONITORING PERIOD I1 EFFLUENT DYEARI MOi DAY IY DAY I N D NO DISCHARGETo j ATTN; Stephanie A. Howard From1 09 05 1 To NU IO: RIeao instructuons oefore completing tnis urtm. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENITRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM ANALYSIS UNITS MINIMUM AVERAGE MAXIMUM UNITI CHLORINE, TOTAL RESIDUAL SAMPLE 27 / 31 GRAB MEASUREMENT
<0.018 0.042 19 0 50060 1 0 0 PEFMIT~ ******** ~ MGIL ~CALCTO REQUIREMENT~ 7 4; 0.10 20.10 *WEEK-I..
'EFFLUENT GROSS VALUE MOQAVG )INST MAX~ DAYS ITEMPERATURE - C, RATE OF .SAMPLE** 1 6******** 0 31/31 CALCTD CHANGE MEASUREMENT6 234 1 0 0 PERMIT ****;2 K DEG ~,~***.4CONTIN 'CALCTDQ JEFFLUENT GROSS VAILUE RDAILY MX C/HR UOUS SAMPLE MEASUREMENT SAMPLE" MEASUREMENT
~RE .. .4.%ýý L SAMPLE MEASUREMENT i~
REQUIREMENT SAMPLE MEASUREMENT REQUIREMENT 4. PEM*. IT ....... . ,- .. SAMPLE MEASUREMENT 4 - -
>PERýMITI<j i 7>j;.444 y 7*74..
'REQUIREMENT I-4 ,tr.
2.... - NAME/TITLE PRINCIPAL EXECUTIVE OFFrICER Idirection Certify under or supervision penalty ofinlaw accordance that this document with a system and designed all attachmentsto assure werethat qualified prepared personnel under my .tt 4 w.,-k* C Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the Principal Environmental Engineer "oa Sinformation, the information submitted is , to the best of my knowledge and belief, true,
- Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVIE
, _,information, including the possibility of fine and imprisonment for knowing violations: OFFICER OR AUTHORIZED AGENT I TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured: 1. H-150M (max. calc. conc. was o.0446mg/L--limit 0.050mg/L) 2. H-150M (low detection level analytical method was <0.020mg/L--limit 0.050mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used .P age 2 of 2
PERMITTEE NAME/ADDRESS " (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name . TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON) TN0026450 101T F - FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NU BIOMONITORING FOR OUTFALL 101 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORIN PERI D EFFLUENT YEAR MO DAY YEARI MO DAY NO DISCHARGE E: ATTN: Stephanie A. Howard From 09 05 01 To 09 105 31 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ** >100.0 23 0 1 / 180 COMPOS CERIODAPHNIA MEASUREMENT TRP3" 0 VALUE................E........45.2'*****,A, . PERCENT .......
<MINIMUM777.7YiWi.~T J1C25 STATRE 7DAY CHR SAMPLE ** >100.0 2.0 1 /180 COMPOS MEASUREMENT 23 PIMEPHALES
ýTRP6C 1 ,0.,0 <PERMITs < 45.<2-:' PERCENT 1> SEE -OPO JEFFLUENT'GROSS VALUE I MIMUIREMPNRMI SAMPLE MEASUREMENT IREQUIREMENTW< *" 4.': SAMPLE MEASUREMENT PERMIT MREASUjIREýME NT SAMPLE MEASUREMENT
-'PERMIT.4::< 4 REQUIREME!LT SAMPLE PER MIT MEASUREMENT
~.f .
MEASUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my.- i ll TELEPHONE DATE direction or supervision in accordance'with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, __423 843-6700 09 06 09 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I CODE COMMENTS AND.EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled 5/10/09 - 5/15/09. Report is attached. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
June 9, 2009 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, MAY, 2009 Per your request, I am only submitting an electronic copy of the subject report. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101, samples collected May 10-15, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Call me at (256)386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment): Sherrard, R. M., PSC I X-C Files, ER&TA, CEB I B-M SQN May 2009m
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE
SUMMARY
Report Date: June 9, 2009
- 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 1,579
- 6. Receiving Stream: Tennessee River (TRM 483.6)
- 7. 1Q1O: 3.491
- 8. Outfall Tested: 101
- 9. Dates Sampled: May 10-15, 2009
- 10. Average Flow on Days Sampled (MGD): 1426, 1443, 1448
- 11. Pertinent Site Conditions: H-150M was iniected into the ERCW B train beginning on 5/11/09 A 1220 through 5/14/09 (a- 1500.
- 12. Test Dates: May 12-19, 2009
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephalespromelas)
Daphnids (Ceriodaphniadubia)
- 15. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101 :11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0
- 16. Permit Limit Endpoint (%): Outfall 101: IC25 = 45.2%
- 17. Test Results: Outfall 101: Pirmephalespromelas: I__C_*>
5 100% Ceriodaphniadubia: IC 25 > 100% UV treated Outfall 101: Pimephalespromelas. IC25 > 100% Page 1 of 107
- 18. Facility
Contact:
Ann Hurt. Phone #: (423) 843-6714
- 19. Consulting ITesting Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 22. Notes: Outfall 101 samples collected May 10-15, 2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC 2 5 values, for both species, were >
100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Minnow survival was significantly reduced when exposed to intake samples during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Page 2 of 107
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101, Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received -) (rg/L) First Used By 05-10-09 0859 to 05-11-09 1510 1.8, 1.8' <0.10 05-12-09 0950 101 05-11-09 0759 05-13-09 0850 Intake 05-10-09 0933 to
- 05-12-09 0950 05-11-09 0833 05-11-09 1510 1.3 <0.100850 051ý09081 o05-14-09 08500 101 05-1-09 0821 to 05-13-09 1433 3.2, 2 .0 t <0.10 05-14-09 0900 05-13-09 0721, 05-15-09 0853 05-12-09 0839 to 05-14-09 0900 Intake 05-13-09 0739 05-13-09 1433 2.1 <0.10 05-15-09 0853 05-16-09 0900 101 05-14-09 0828to 05-15-09 1428 2.3, 1.5' <0.10 05-17-09 0920 05-15-09 0728 05-18-09 0903 05-16-09 0900 Intake 05-14-09 0842to 05-15-09 1428 1.7. <0.10 05-17-09 0920 05-15-09 0742 05A18-109.0903
*TRC - Total Residual Chlorine t
Samples were collected in two 2.5 gallon cubitainers. Temperature'was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.00 C) in a warm water bath.
Aliauots of Outfall 101 and Intake samtles were UV-treated through a 40-watt SmartUV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Page 3 of 107
Pimephalespromelas Ceriodaphniadubia Test Organisms:
- 1. Source: Aquatox, Inc. In-house Cultures
- 2. Age: 20.33 hours old <24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate: 10 1
- 6. Test Initiation: (Date/Time)
Outfall 101 05-12-09 1220 ET 05-12-09 0950 ET UV Treated Outfall 101 05-12-09 1232 ET
- 7. Test Termination: (Date/Time)
Outfall 101 05-19-09 1123 ET 05-19-09 0854 ET UV Treated Outfall 101 05-19-09 1140 ET
- 8. Test Temperature: Outfall 101: Mean = 24.7 0 C Mean = 24.9°C (24.2 - 25.1 C) (24.6 - 25.2"C)
Test Temperature: UV-Treated Outfall 101: Mean =24.7°C (24.3 - 25.0 0 C)
- 9. Physical / Chemic al Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in. each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposuretestsolutions Were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
Page 4 of 107
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration) Conducted May 12 - 19, 2009 using effluent from Outfall 10 . Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 `100 100 100 100 100 11.3% 100 100 98 98 98 98 98 22.6% 100 100 100 100 100 98 .98 45.2% 100 100 100 100 98 98 98 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 98 98 98 98, Intake 100 100 90 68 60 53 50 Test Solutions Mean Dry Weight (mg) Tet Soluetis (replicate number) (% Effluent) 1 2 3 4 Mean Control 0.730 0.684 0.895 0.761 0.768 11.3% 0.753 0.655 0.723 0.717 0.712 22.6%. 0.654 0.723 0.673 0.760 0.703 45.2% 0.646 0.684 0.638 0.649 0.654 72.6% 0.665 0.632 0.656 0.603" 0.639 100.0% 0.723 0.604 0.700 0.641 0.667 Intake 0.212 .0.285 0.496 0.567 0.390 IC 2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95'% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa I00/LC5 0 : TUc = 100/IC 25 Page 5 of 107
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic/ 7:day Toxicity Test.
(Genus species) (Type / Duration) Conducted May 12 - 19, 2009 using effluent from Outfall 101. Percent Surviving Test (time interval used - days) Solutions _(% Effluent) 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6%' 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (%Effluent) Data (replicate number) _%Eflunt 1 2 3 4 5 6 7 8 9 10 Mean Control 33 34 33 34 34 32 34 34 31 31 33.0 11.3% 33 36 32 32 36 33 33 34 33 32 33.4 22.6% 35 36 34 3'6 .32 33 35 34 33 33 34.1 45.2% 36 34 31 35 35 33 33 34 33 31 33.5 72.6% 37 37 36 32 35 32 31 34 34 33 34.1 100.0% 39 38 32 36 34 33 34 35 .34 31 34.6 IC25 Value:- > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Pennit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LC5 0 : TUc = 100/IC 25 Page 6 of 107
--N TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration) Conducted May 12 - 19, 2009 using water from Intake Percent Surviving Test (time interval used - days Solutions 1 2 3 4 5 6 7 (% Effluent)- Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) Tet Soluetis Data (replicate number) (%Effluent) 1 2 3 4 5 6 7] 8 9 10 Mean Control 33 35 33 35 34 33 31 31 33 -33 33.1 Intake 39 35 41 36 35 38 37 36 37 34 36.8 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = I00/LC50 : TUc = 100/IC 2 5 Page 7 of 107
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration) Conducted May 12 - 19, 2009 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3- 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 -100 100 100 98 45.2% 100 100 100 100 100 100 100 72.6% 100. 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 -100 100 100 100 Test Solutions Mean Dry Weight (mg) A, (%Effluent) .(replicate number) 1 2 3 4 Mean Control 0.637 0.743 0.695 0.659 0.684 11.3% 0.775 0.852 0.756 0.772 0.789 22.6% 0.737 0.727 0.706 0.653 0.706 45.2% 0.693 0.722 0.683 0.756 0.714 72.6% 0.682 0.668' 0.665 0.722 0.684 100.0% 0.631 0.664 0.751 0.745 0.698 Intake 0.695 0.635 0.662 0.642 0.659 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LC 50 : TUc = 100/ 1C25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)____
TEST RESULTS (see Appendix A and D) Species REFERENCE TOXICANT Date I Time I Duration I ToxicantI Results (1C2 5) Pimephalespromelas May 12 - 19, 2009 1208 KC1 0.75 g/L Ceriodaphniadubia May 05 - 12, 2009 0905 NaC1 1.07 g/L Page 8 of 107
.. .. i.._ ........
,. .. .. :.. .. ... . . .. .. .. ... .. .. .. :.................. I ........
I ............................................ : . ..................................................... ............................................ ........... ............. ............................. .. ... .......... PHYSICAL/C HEMICALSUMVMARY WaE r IC Ae S U MM an .alu s. a d....e.f.r...es.................. Water Chernisr Me yan ales and Ranges for Pimephales.prolasand Ceriodapniadubia Test, Non-treated! SequoyahN uclr Plant (SQN) Outal 101 ............... . ................. N r...o..........
.. .. . P. .................. ................ ....................
performed May 12-19, 2009. Test Sample ID Temperature (C) Dissolved Ox ygen (r/L) pH (S.U.) Conductance Alkalinity Hardne s s Total Residual Initial Final Initial Final Initial Final (inihos/cm) (mg/L CaCO3) (mg/L CaCO3 ) Chlorine (mg/L) C o n tro ll ....... 24.7.
....... ....... ."..... 24.6
....... ....... .... ... .......7.6......... ..... ........ 7.4 I........ ....... 7.55
....... .............. 7.25 .......
.............. ....... 321....... ............... ..............
61 .............. .............. 89 ...... ....... ....... ....... 24.7 24.8 24.5 24.8 7.4 -: 7.8 6.9 -.ý 7.5 7.32 -, 7.66 7.04 :-: 7.44 302 -: 338 60 -! 61 88"- 92 11.3 % ...........24.7 .: . . . . . . ......... 24.6
. -- - .... .................... 7.7....... .................7.3 7.41 ... 7.22 297 ..........
24.7 24.8 24.2 - 24.9 7.5 -: 8.0
- 2. 6.9 "..7.5 7.26 7.50 7.01 - 7.36 284 -.. 306 _____________ ____........
24.8 24.5 7.7 7.2 7.40 7.20 280 E2 22.6% ...24.7:...... 24.8 -24.2 2*15 75 .. 81. .. 6.8 .. 7.5 726-: .. 7.49 7.03 .... ....................... 7.34 268 287 S4 '5 .2 % ... 24.8 .............-.- .. 24.6.. 2..
... .......7.7 .....
- 4. ... .... 7.2 . .....-714......
....... 7.37 1' ..........
3'......7.
......... ..7.19........................
4...................... 243................ ....................... 5 '..................... "..................................... _____24.7
- 24.9 24.2 - 24.8 7.6 8.0 6.8 74 .23 74 69 73 24 27 _____
24.8 24.6 7.8 7.2 7.33 7.17 198 727 ..6% .............. ............ ...............................................
- __24.7- 24.9 24.4 - 24.8 77 7.9 6.8 75 7.18 74 7......7734 697 7i 189 ::%.205 24.8 24.6 - 7.7 7.2 7.27 7.13 150 57 62 < 0.10 100"0°/ 24. 25.0 24.4- 24.9 7.7 .78 6.8 7.5 7.14 7.39 6.95 7.24 145 156 50 - 65 60 - 64 <"0.10- <0,10 24.9 24.6 7.8 7.2 7.25 7.13 147 54 63 < 0.10 24.6 ::-:25.1 24.4:: 24.7 7.7 7.9 6.8 7.5 7.11 7.39 6.97 7.25 143 153 52 ' " 57 60'"-: 64 <0.10:- <0.10 Cooln tr 24.8
... -: .. ..... 25.0
... :.: .. 7.6....""" "-'...................
7.6 ...................... 7.55.--..-- 7.39
.. ... T ' " '
321 61 ................. 89
" " .. " ¢T............
'"24.7-"25.0 24l 24.8 -"25.2 7.4 -: 7.8 7.5 78 732 766 7.21 . 7.47 302 338 60 -: 61 88"-'92 11.3 %/ . . . . 24.9 .." .. ...........
.... . . . 25.0 .". . . . . . . .7.7. . . .. . .7.6: . . . . . . .........7.41 7.40 ... ... ..........................
297 ."..................................... 24.7 25.0 24.8 :-: 25.2 7.5 -: 8.0 7.4 7.8 7.26 7.50 7.22 -! 7.49 284 306 _ 2 2 .6 % ...24.9
.. .. - 24.9 :.... ...............-...... 7.7
....................... 7.6........... ...........% 7.40
.:.............. 7.39 .... .... ... 280 24.7__ 25.I 24.8 -:25.2 7.5 -~8.1 7.4 7.7 7.26 7,49 7.22 7.48 268 287 _______
24:9 25.0 7.7 7.6 7.37 7.38 243 4 .2% 24.8 ..... :4 :, 25.1 . 24.8-: ............. 25.2..... r"": 7.6 ": ........ 80. 7.4 7.7 7.23 ".........
- .... .. ":......I...... 74 7.22... ........... 7.48 234
..................... 247 :
24.9 24.9 7.8 7.6 7.33 7.35 198 1% 0. ......... 24.9 24.9 7.7......
. ..... .. I . .... ... :-...........
7.6.. ....... i . . . 7.27 . .....
. . .. ..... ..7.31 .. . . ... 150.. . . . ... . . . .... 57I . . . .. . . .. . .. 62 ...... . . . . .. ..- .< 0.10. . . . . ..
____24.8 25.1 24.7:- 25.1 7.7 7.8 7.5 7.7 7.14 739 7.20 7.38 145 156 50 ::ý65 60 ý- 64 <0.10-: <0.10 Iit.n a k e . . 24.9
. ... . . . .. . . . . . .24.9 ... ... . . . . . . . .... 7.8
. .. ........ ......... . .7.6 . . . .. 1. . .. 7.25
....... ..... . .... . 7.28 ....... ....................147 ......- ... ...................54............. . ............. 63............... .............. < ..0.10 . ...... ....... .
___24.8 -25.1 24.6- 25.1 7.7 79 75 77 711 739 709 7.35 143 153 52 57 60 - 64 <0.101-ý <0.10 Overall temperature Average Minimum Maximum Pim I.ephales.. ............. promeias %...i~ . . . . . . . ....i24~ ... . . . i.. . . . . 24.2 ... ...... ....... 25. 21 ........... .... . . ........... .............. ..... ......... ... ................ .... .................... .......... .............. .......... Ceriodaphniadubia. 24.9 K 24.6 25.2 Page 9 of 107
- PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry.Mean Values and Ranges for PimephalespromelasTests, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed May 12-19, 2009.
.. .. .. .. .. ... .......... ... ...... ..... .i. ... ............... .... ............... ii.......7 .... 1i i.............. ....... ......... ....... ........
.... . .............. i.............. ............ . . . .i Test Sample ID Temperature (°C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance. Alkalinity .............. Hardness ......
Initial Final Initial Final Initial Final (pimhos/cm) (mg/L CaCO 3) (mgIL CaCO 3 ) 24.8 24.6 7.7 7.5 7.44 7.27 314 60 91 C 24.7 - 24.9 24.4 :- 24.9 7.5 7.8 7.0 7.8 7.28 7.53 7.04 -. 7.44 296 334 59 61.6 88 { 92 1 1 .3 %/ 24.8
.. ...... ..... .i-:
25 .0.. .. 4...... 424.6 i- 4.. . .....7........ 7.7 .":-............ . 7.3
. .............. : i': ............
7 5 ..... 7 2.......7.45-::.............. 7 . 3.0......... *. 87.25 732 ............ 2 302 ........
- 3 2.................. "{........ ............... ...... ..............
_____24.7 -. 25.0 24.4 -24.8 7.5 -~7.9 6.8 -~7.5 7.29 -. 7.53 7.08 -7.38 282 -321_______ ______ 2 2 .6 % ..... . . 24.8. !... ..........
... .. . 24.5
. .. .. . . . . .. 7.7 .. .............. , .......... 7.3 .. : . . 7.45 . ... .. ........................
. ... 7.23 ......... ..... 282 ............
24.7 .-. 24.9 24.4 -: 24.7 7.5 -{ 7.9 6.8 7.5 7.29 - 7.54 7.02 - 7.39 264 299 .......... '".......... _ .. 24.8 24.5 7.8 7.2 7.42 7.21 246 4_____24.7
- 52. % ............ , -~24.9
............ :........... I... .................
24.3 -24.9 7.6 -~8.0 6.8 7.4 7.28 -7.51 70 7.8 231 258 24.8 24.6 7.8 7.2 7.38 7.20 200 24.7 -i 24.9 24.3 -: 24.8 7.6 8.0 6.8 7.5 7.23 - 7.49 7.03 -: 7.36 189 207 _ '"_ _____ 100.0% 24.8 24.6 7.8 1%0. .......... , . . ..... .......... ..... '" ............. . .. , ,.. ...... .....7.2 . .... ... ................7.33 " ........ .. 7.18
... ....... 153 ........... 50 ...
...::- 5... ...... 0!....62.. .......
24.7 -:25.0 24.3 24.8 7.6 -80 6.8 :75 7.19- 7.43 695- 7.30 147 159 44 54 60 64:": 64 24.9 24.6 78 7.2 7.30 7.14 149 53 62. .... I nta k e 24 7..- 2 252.
.7 0 . . 6.8 - 7.5 7.15 ..7...
... . ... . . ...... ..... .... ............ ........ 5 . 43 6.8 . 7.2 141 i i* ......50 -5*......
*i-72 .2.. ....... 57.. 8 7 5.6. ...... 66*
.......... :. ............ : .. ............. I.......... . .. :. . ............. . ....... ... .............
Ove rall temperature C(..) Average M.inim.um Maximum .............. ... .............
...... ... .. . . . . . .Pimephalespromelas
. .... ........ .... ..... ............................ .......... ....... 24*..........
..... : ...... 7 ...... ....... 24
...... 3
.................... 25.......0 ...... .. . _
Page 10 of 107
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected May 10-15, 2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Minnow survival was significantly reduced when exposed to intake samples during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
.2f Page 11 of 107
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephalespromelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia Tests were conducted according to EPA-821 -R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None Page 12 of 107
PHYSICAL AND CHEMICAL METHODS I. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibraition and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B.
- 4. Dissolved oxygen was measured by SM 4500 0 G.
- 5. The pH was measured by SM 4500 H+ B.
- 6. Conductance was measured by SM 2510 B.
- 7. Alkalinity was measured by SM 2320 B.
- 8. Total hardness was measured by SM 2340 C.
- 9. Total residual chlorine was measured by ORION Electrode Method 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted-according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as 1C2 5 values in g/L KC1 or NaCl.
- 2. Standard Toxicant: Potassium Chloride (KC1 crystalline) for Pimephalespromelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 13 of 107
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Standard Methods for the Examination of Water and Wastewater, 2 0 th Edition, 1998.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of 107
Sequoyah Nuclear Plant Biomonitoring May 12-19, 2009, Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 107
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 10O1) Discharge Concentrations of. Chemicals Used to Control Mi crobiologic ally Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 15, 2009. Date :i:i:::::i::iii :iiiiiiiiiTo ebom i*i::i:* * ::ii!: C -0 i::i iiiCuprostat- : 4.ii0.M i~iiii
! iiiii m g/L iiiiiiiii {iiiiiiii mg/L ii!i{i iiii PF iiii ~ iii
.............. T*ii !iii**- .................... Az l 0ii} 1/ 9 8* * ;***((;***........................
3 {!{:i{*i~ .............. .. .iiiii~ii 9 0 / 9 9~ i!! ! !*~i*iii 0i~!*!ii~}**!{i!i!*** ............ . ............................ ........................ ii::iii~i::i:i:* !* {iiiiii .................................. ::::::::::::::::...................::::::: 03/14/1998 * *i!~~i!i{iiii iii iiiii:~!!~i~ 00 9 :::iiiiiiiiiiii~iiiiii ..... .......
~~~~~~... . ............
- i 0 01 *i-*}iii
.......................... i i -- -ii)
~iiiiiiiiii 09{i/ 2/ii ii~iii;ii~i(( ii 99ii((i~{
- : : ::::::::::::::::1:
0 3/ 16/ 19 9 8 :::::::::::::::::::: ... ........ ::::~~::::::::: ::~::::
....................... i****5iiiiiiii!iiii
/2/ 199 i{i* i....!...... .
I0{ii~~i}i~ii~~i i~~iii((((}(()ii~ii! .............................-............. ii~ i~iiiiii* i~~i{!i}iii~~i!ii~!{i~
]8/1 il
{}'i~{'{i(({i~ii)i! 08-;.*){99 0 01 i~ii~i~i((i~ i '* 'i.i::*}iil'!i::{} {;}i 0 024 ::i..................... 08 1/199 0 . 12 **i{ii((i *iii(( 0 0 24 ::i~i~i;:}[~i~
..i~i~iii.... :::::::........::::::::...::::.....:::
08/20/1999 0 .023 i iiiiii* i ~ ... ~
.i.li.}.i.} 0 ..2 . .....
- 0 1 0 iii~iiiii~iii{i 08/21/1999~~~
((((((((((((iii{i{}ii~ii~{ii~}iii{ 2 i~~ii{ii
.. .. ~~~........ ..
002..-:......iiiiii~ii~iiiiii
.. .l ...... ....
0982/9 . 2 {.......il!i .00 4 :::::::::: : ::::::::::::::::::::::::::::::::::: . .... Q 08/4/1099,3 1 / 1 9 ..... 0 . 16 ii........!!* .. .
* ! .{.ii .. .
.i . 2 i:i}:ii 2* :ii:i: ::::::::::::::::::::::::::
.. :iiii:::: ..............
iiiii:i! Page 16 of 107
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbi ol ogic ally Induced -Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 15, 2009 Dat ~ e~~ i ~i:ii~iii:i:i: ~ T ~w r r~ m}iiiiiiii ~ ..... iiiiiiiiii CL -4 0 1 iii*{ C u pro sta t- i i *0 iiiii*iiiiii mg/L iiiiiiiiXii~ii~~i~ mgL iiiii! iiiii{ PF iiii~ ii
*ii!*~~~***~*.TRC i**~~~i~iiii~iiii!~ iilii* iiiiii Copolymer :::: :! mg/L iiXi!i*~
01/31/2000 ***~ *;;!;iii~**{~: < 0.002 0iiii;i[ii2i~iiiiiii 009 iiiiiiiiiiiiiiiii}i 000 1i}iiiiiii:iii~iiiiiii:i::iiii~iiiiil~i~~iil 02 2 0 .0 11 .!..iiilii!* ii..[i.ii 0 .028 ........................... ~ ~ ::~~::*!i!sii!i 02/03/2000 **ii!iiii*iii*i*i{iiii;ii. 0.008 i~N ;..[*ii Xw,!~ 00.. .........~iiii:::::::::::::::::::::*3:*:3*<*3: 02/04/2000 0 06 iii*;{* N !i!*!i 09 :::::::::: : :::::::::: 00:::::::::::::::::::::::::009:::::::::::::::::::::::::::::::::: ....... iiiiiii~i~iiiiii 0020/2 0 .0 :................. .:::::................. 072/ 00 0 0 5 iiiiii~ii~ ~ iiiiii....... 19.......!iii~ii!*i~iiii ..................... 0 7 /27 /2 0 00....
........... .............. 1 i~iii~ i ........... . . . . . . ... .. ::*:i:::**::
............................. iiiiiiiii iii*iiii ........................
- .) 0 . 19:::::::::::::::::::::::::: ...........................
i~i** 0. 076
- s*
- s*
07/3 /200 ii*;;*iiiiii*)!!*!!;*i*i~~ii~i~~ii 0.0 9 :3*3*3*3* ;3*; . ............................ 07/26/2000 < 0.0057 0.019 ;:i:i:**:::i:ii:!i~:g:g:*ii::i:i:i:i 07/27/20 0< 0.0141 ::: ::!l:: OS::::: :::::....:::: 0.019
...... liiii N ii*!* iiiiiiiiiiii!iiiii 12/11/2000 .... .. ... 14 :::::::: : :::::::: . 2 i:i:i:~N *:i:::i: ......
07/128/2000 .... 0.0088 ii~*iiiii*i 0.018 5-.3{i~!!{i "j) iii 0.108*N*~* 072 91 /2000 i*iiii*Jiiiiiiiiiiii**iii*iiiii*iiii i < 0 .0 208 ....... ..~iiiii!*!! 0 .0 19 :::::::::::::::::::::::
- !*i:*i~iiiii~.............. .. ..
0 8 /017 / 20 0 0 i.i:*.:*.:i~i. .:!.:i.! ........i.ii:;!.:!.:< 0 .0 0 31 41 iii~i* * :i:~i:i:i::! 0 .0 19 ................
/202/10 0 8 2 1/200 ii~i~iiiiiii~ .0 7 iiiiiiiiiiN !iiiii~iiiii
'....... ...... .. 0. 2 iiiiii! ;~iiii ......................
12/27/2000 :::,*'~ i!{!i{**~ iiiiiii*!*iiiiiiiii* .00:.:.iiiiiiil* ~ ii~iiiii 0.020 iiiii @i:~~i:::::::::: ::::::::::
......... ...... .. ............. i*:*>
............... :* :T ii}:ii:: ::
* ....................... ................. .0 I i 17 1 *..0.....::: ......................................
- : :..::::...: - iiii!ii!T iiiiiiiii7 120/0 13/2 0*, 1/2/20,** i!!::ii !,, <<....................................
1*:,::::::::::::::::::::::::::::::::::::
- : :::::::::: " 77{7iii*Xii~ i
*}i*:i~
* / 72:::*:ii:~~i:*~i~:i:ii::::ii:*:}i:::~ . 13 :: ::::::::::::::: . 1 112 /2 0 .0 0 87~i*::5 ..............-
<:::0 ". 0 .0 20::55*5:5:::: :+.. . .:-+
.*i5*J . .:-.:-..-----:::::::::::::::::
11~ ~0 ~ .......... 3 ...... {* ~~~ii{i:{ . . .0 02i;:{ii +.. {..........
.. :i:i:::i
- ~*iiliii}iiiii*i~~-iiiiiii((*~i:ii~:i ~((!****i!i!{
.< . 02 3 iii~ii(({i{
12/ 1 5/2 0 0 0 :::::::::::::::::::::::::::::::::::::::::::::::::::::: 0 i .01 ii((iii~{i, ::::::::::::::::::::::::::::::::
- ....:i:' "....:::.:::.:: ::ii7i:::~
.~iii7::
12/16/2000 < 0 0.0036 42 i% % 11;i; 12 1112iiii:ii~iii~iiiiiiiiiii.......... .020ii!i*!{!i*!}:.::::::::::::i:i:ii:::.I
" :ii~i::i::2:i:?:::: i~ii~i~ ~i:::*:**
~i~
~~!!*3*!i :::::::::::::::::::::::::::.:..: :?::i~..:.:....
*:i:iii:: ....
i!:*
;:::iiiii 001~~~
2t10/2*i:**:**{ * :3*:i*:i:::;i{i 0 0 4 2 - ~~ i~iiii**!i
.......... .0 ..
- iiii::::~i 12/17/2000 2/12/20 0 1... s3; 33m #~
~ i!*i*!~iT!*i*!!7**~i*
12/11/2001 ~ 0. 03 ~ 4.
<ii0...........ii ~ i 0
il~iii~iii 0.:::::::::::::::::: . 2 ~ .3333**~ [i~
- 7:::::::::::::7:::::::!:::i!:::i 020.........................................1
~i !* :::::::::::::::::::::::::
12/13/20 0 1~~ ~~~ .iiiii~iiiiiiiiii*~~iiiiiiiiiiiiiii < 00..:..::.::::.:....::::.::.:::::: . 2 m35:!***!!! iiiiiiiiiiiiiiiiii iii* !ii:i:iii:~iiiiiiiii:i 00 3 21!20 ::::::::::::::0:.:01:7::::::0:.021:::::::::::::: 0 8/:26:/200 1 1:i:i:iii:iil!:;~ ii:; :!!!:*...
.... ii}i:i::i0
- 0::::::::5 :: 0 ..
. . . i. . .. .iiiiil.........................
iii;i iiiiiiii iiiifii Page 17 of 107
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- May 15, 2009 Date .Towerbrom C 222 :: PCL-401 XL-363 Cuprostat-- H. IOM Wit/.. mgmg/L mg/L PF gL
........ .. TRC hsP bt::::X::::: Copolymer :::: DM D .:: .:.:::::..
X:.:::::::::: mg/L .Qut i!i*i * .....!?
....... Azole 01/02/2002N.."'.."" < 0.0079 .... 2... ..... 0.02 0,006:X.-.: X.
01/03/2002 ..
.. < 0.0042 .. 0023
=====================
`0".02.3!*!!!i~~i
- i*ii*!!i!i!*~
- ..... 0.014 .............. . . . ...... -...........
08/06/2002 <0.0014 iý' 002.""' 0.018
...+ ..
......: ..... :.. .... ':.......... .* ~
*i i !i~ iii i i i i i !;* *: *** ! i i i ?i 01/12/2003 .::0::0035: .......
8 /000 2.. 05/2 <0 0 5............iiii*i . 1 8:::::::::: :::::::::::........................ iii~ *
.... ii 01/13/2006 .:.: :....................... ...... .
0.02.. 020 : : : : ::0..*... 01/15/2003 <0.0063.. .. ........ :: :::: ::: ::: : ::: : :: : :: :: 10/06/2002 ... <0.049..... 10/08/2002. 0..2 0.0.8..... .. ... .. 01/06/2002 08/07/2002 < 0 3
<00.01041 iii'i 042
- : : $.ýýýý
*iiýiýiiiýi
- 00850. 0 020..........
1 ::::::::::::::::::::::::::::::::::::::07iiiiiiii~i*iiii
~ ~iiiii ii!:ii:iiii:ii~l
............. i.:i 9/2 00 2 i~iiiiiii~i~iiii!
08/0 . i*iii*ii~i~ii*ii:!3 ..... :::::::::: : : : :::::::::::::: . 1 8!iiiii~ 02 0 / 10/11/2002~ 02/27/2002 *0.0042
< 0 4 0.4001 0ii**i~ii~ii~iiiiiiii~~ii!~~i 0 (123: 0.023::!:::iiiiiiii 7 iiiiiiiiiiiiiii!**iiiiiiiiiiiiiiii.004i.:il~~~:.*::iiii~~~~:i -.
08/09/2002 ,.iiliii~iili!!~!!~l!i;~~iiiiii~liiil <0.0 01501/17/20 30.111. <054!~ N :ii:li:iii:i 0.018 .0. ...-.....- ...-.- 11.008*::::*::**:;*::*:! !!!:*.:*:*::*::*:*::::*: 01/14/2003. <0.011 ~ 0 2 ... 04/076/2003 <0.089 ...... .............. . . . .
. 0021 ... .......:::::::: : :::
04/06/2003 <0.0073.04 6
................ ,............. :.....+ + + .............. :: :: .+ + .... +
05 /0 5/2 00 2 i!~i**~i~ ii******ii;i~:!~iii*iii <0 0........iii i } ii~i~iiii ....
. 2 0;iiiiiiiiiiiiiiiliii:ii!-::::::::::::::::::::::::::::
04/08/2003:.:. 05/ /06/2002 3!*::!*!i!i:i[ii~iiii: <00.0117 0 6 *iii~ ~~~* ......... 33i~i~i 0021... 0.02
. 20[i*~i iiiiii ii:i**:ii*i~iiiiiiii 04/10/2003..... <001- 01 01 04/21/200 <0.0073
.............. .i..
-0.022
.:i*3.!i ii!i!!!~ ...... i~
...............)*!:...
S . . ..., ...: ..... =.... .......=============== 05/08/2002 :*~!::::::i::iiii:iii::i::::::i~!:* o0 7 :;:ii::i::i::i::i:::~ :i:i:i:ii:iiii:i:i0 0 2:iiii:i~i::~:iiiiiiiiiiiii::i!ii;* ........ iiii:i:}i -Page 18 of 107
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations' of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 15, 2009 D ate iiiiiiiiiiiii::i::iii::]T ow erb rom i ii *iiiii P CL -4 0 1 *ii:i::: C u rostat- ::H4 3.0-M. i~ii..ii((iii~iii
.... . ..... ........ i:::iii~i~iiii ii~((!{i~ii~il~iiiii A zo e ::::**{*ii..............
06/15/2003 ii< 0.0045 .................... ....((i 0. 14 ::::::::::::::::::::::::::::: i~i{* a i((i 08/ 3 / 00 *< 0 00 0 ... .. .... .......... ..................... ;; i*i::i:}:}i:i:} * : 06/16/2003* < 0.0037 0.020 *!i{*{i*;i{iiii{i{iii :::::::::::::::::::::::::::
!*
- 7*; i*!ss;;ii*i!**!*i*# ..............
.......*i .......................................
06/18/2 00 3 Xi{i!**ii*;*iii{i*{ii*iii{i{i~~~~~~~*i~~
........... 15 :: : ::::::::::::::::: *X.0 .0 14 ....... :: ::::::::::
- .::::::: ...... !i~~~ii~*!!
0 .0 0.. .......
'6 4 4* ! !i~
.............. I.......
!!!!l~ : E::i~i*
0200 84 3 ...........................................................................................
..........:::::::::::::::::::: .. 03....::::::::::: : : ::::::::::::::: . 00
.. ............................... :i~l{ii::!::::@ :i::i{ii:- :i:*ii:**~ * ? :i{ii 08/07/2003 <0.01239 ) ) i~ iiii!;ii 0.020 ............. ............... : i i:ii:
08/0 8/2 003 ::::X:::::::)::::::::::::::::::::::::::::::::::::::::::XX
- 0 .0 153 MASi*{i*}i{ I .iii((~**;i . 2 , i{i((*
- i{i{' 'i~~~i i ii{i
............. :::::::::::: :::::::::::::::::::::::::::::......:.*[!*::) :(({:::~i)~
Page 19 of 107
Table B-Il Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- May 15,2009 Date Sodrnm. Towerbrom PCi iii:i PCL-401 Cuprostat-PF H-t30M NL-363 Nalco Rt Haypo00eh WAt mg/i ...... . mg/L igL mg/L 7355]
.nL
.....-i........ TRC.
........... . P hpa . Copolymer :D.............. A : zole ..... Qrt ......... mg/i Eo/Po .
11/08/2004 ....... <0192 0.030 ..... .... 11/09/2004 <00..2 0.016 .. ........... 11/107/2004 <0.0149874 0.0164A4 11/11/2004 0.014 3
*iiiii~i!iiiii}~iii~~iiiii i 49 iiii OXl)i~~i 0.017 ::::::::: 43t::::::::*********
OA*********::::::::: 11/10/2004 02/0/205.. ............ 0.....00.3149
<004 ~ 2 ....... ... . 0.010 ......
11/11/2004 02/07/2005 .... <0.01849
<0.0116 0....1...... -W 0.010.... ... 0007..
07/19/2005 : :0.0263 : : ... 0.0 0.010 ........ 02/08/2005 <0.00023 02/01/2005 0.0100 0.0042
<0.004 .1 ..... ...... 07...
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Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 15, 2009 Fowerbron PCL-401 Cuprostat-PF Nalco mg/L mg/L mg/L 73551 TRC Copolymer Azole mg/L EO/PO 0.0055 7-0.0068 0.0143 0.0068 0.0267 0.0222 0.0188 0.0138 0.0120 0.0288 0.0376 0.0187 0.0084 0.017 0.0103 0.0164 0.017 0.0305 0.0241 0.0128 0.0238 0.0158 0.0162 0.0175 0.0039 0.0124 0.0229 0.0143 0.0120 0.0149 0.0260 0.0151 0.017 0.0172 0.0154 0.018 0.0086 Page 21 of 107
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 15, 2009 [owerbrorr PCL-401 L.*3'.63":*iiii'il Cuprostat-PF iff i~ Nalco MSW mg/L iiii~~i iiiiii; mg/L iiii~ iiii 73551
- mg/L 101 TRC Copolymer !!i iiii Azole iii iiiii mg/ mg/L Phosphate 0.0197
.0.0237 .............. -.........-...-..... ..........:::::::::::::::
0.0104 ............. 0.0155 . . .................. ,............ ...::::.........::::::::...: 0.0106 -.. . . .................-......
- .... ..+ . . .:. .:
0.0129 0.0415 ...... ::: :::::: .. .:.:.:..:.:.: ..:.:.. .. 0.0053 0.0049
<0.0141
<0.0160 Page 22 of 107
Sequoyah Nuclear Plant Biomonitoring May 12-19, 2009 Appendix C. Chain of Custody Records and Toxicity Test Bench Sheets Page 23 of 107
C BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 -4 Cliemt: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity "351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comments-/ 1 J),f- ' Chevy Wilas NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell : v k.*
- Adam Deiml-in Collected By: Chevy Williams, Ben Mitchell, Adam Deimling Fax: 828-350-9368 Metals Analyzed. Samples remained on ice through out sampling and transport to lab.
Field Identification/ Grab/Comp. Collection Date/Time Container Flow Sample Description Number & MGD Rain Event? Volume (Mark as Appropriate) Laboratory Use Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear. (EST) .. Inches Number Temp. ance SQN-101-TOX Comp 05/10/09-05/11/09 0859- 2(2.5gal) ,, Tl i,01 .j... ' L '\So SQN-INT-TOX Comp 05/10/09-05/11/09 0933-
~~0833 1 (2.5 gal) NA , 0,0( 90S1-0.. l0 e Sample Custody - Fill In From Top Down -- o es0- ,
*6L Relinquished By (Signature): Date/Time Received By (Signature): . d%(me Chevy Wil 05/11/09 Sonic Delivery 05/11/09 Sonic Delivery 05/11/09 ETS 05/11/09
_____// E- ' Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 60 C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
cr~ u~I 0 BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 I q
-1 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Commen Chevy Williams:/ [,1 1 J NPDES Number: .TN0026450 Phone: 828-350-9364 Ben Mitchell: Adam Deimhng: _ _ _ _ _ _ Collected By: Chevy Williams, Ben Mitchell, Adam Deimling Fax: 828-350-9368 Metals Analyzed. Samples remained on ice through out sampling and transport to lab. Field Identification / Grab/Comp. Collection Date/Time Container Flow 'x .. +/-: 5A4 4 Li Sample Description Number & MGD Rain Event? LaboratoryUse Volume (Mark as Appropriate) .a. Collected __._ Date Time Yes if Yes, No Trace ETS Log Arrival By Time Appear. (EST) InhsNumber Temp.nc SQN-101-TOX Comp 05/12/09-05/13/09 0721 - 0821 2 (2.5gal) j*7' / Y'-:.*2.20C* L2-2 1"" SQN-INT-TOX Comp 05/12/09-05/13/09 0839- 1 (2.5 gal) NA 3 . ,\ ". Sample Custody - Fill In From Top Down - *r~ 4 d A-
- Relinquished By (Signature): Date/Time Received By (Signature): - Date/Time Lo Chevy Williams/ . - 05/13/09 /( -3!5 '6S-7-Sonic Delivery 05/13/09 SonicDelivery Sonic Delivery 05/13/09 ETS A1 4~ 05/13/09
, to*A3
'I J.
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6*C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
ii Page 1 of I BIOMONITORING CHAIN OF CUSTODY RECORD
.3 -
0 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery 28801 General Commen ts/t./ 4 . Facility Sampled: Sequoyah NP Chevy Williams. NPDES Number: TN0026450 Phone: 828-35,0-9364 Ben Mitchell : Adam Deimling: Collected By Chevy Williams, Ben Mitchell, Adam Deimling Fax: 828-350-93-68 Metals samples taken. Samples remained on ice through out sampling and transport to labI Field Identification / Grab/Comp. Collection Date/Time Container Flow eCULP Volume (Mark as Appropriate) PfAi-Collected "__ _*.. .. .... __ Date Time Yes If Yes, No Trace IETS Log Arivl By Time Appear- __ -, Inches Number Temp, . 'ance SQN-101-TOX Comp 05/14/09-05/15/09 0828- 2 (2.5gal) is-f " oq0 . LI.s".. -... 0728 1449-3. SQN-INT-TOX Comp 05/14/09-05/15/09 0742 0842- 1 (2.5 gal) NA "ýI " .0.L* 6 _, [ -' " ",rt ..I ... r " ' Sample Custody - Fill In From Top.Down -* W o _ _ _ -. _- Relinquished By (Signature): Date/Time Received By (Signature): . D~/Tine Chevy W 05/15/09 Sonic Delivery 05/15/09 Sonic Delive 05/15/09 ETS 05/15/09 SonicDelivery!Q; Z r f"K -- **&1Z*2 1'_Z *- Instructions: Clients should fill in all areas except those in thle "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be markedfor Saturday delivery or they will not arrive until the following Monday.
- Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA County: Hamilton Facility: Seuo-yah Nuclear Plant Treatment: Non-treated NPDES #: TN 0026450 Outfall: 101 Project #: :,4ýAA Dilutionprearationinformation: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organisminformation: Test information: Organism age: 2o.-15 AQuA OCb Randomizing template: "ILOg Date and times organisms 0.%- 0o%16,0 Incubator number and were born between: shelf location: _-___ Organism source: N-,-f- %'Mty\ Pe 0,- 1v-(A Artemia CHM number: CARI4'4I Transfer bowl information: pH = SU Temperature = 'C Date / Time in: .oG Ir-z-oc>
-. "-*. Date/ Time out: os.1m.i i,.L7o Average transfer volume: Total drying time: -- .tIS Au
- 0. "OS* -*- Initial oven temperature: to '(...
..... _ _ _ _ __ __Final oven temperature: ,
Dailyfeeding and renewalinformation: Day Date Morning feeding Afternoon feeding Test initiation, renewal, Sample MHSW or termination numbers used batch used Time Analyst Time Analyst Time Analyst 0 o -0 _____ v0 .o iAoo 177- C .OSIk-Go -0LO-CPP I__ os-IS-0'1 0&00 A.~ '4Po &4 IiA 0j.1aw - o -kO os-aAA 2 0---A at A 11 3C k I _ý ORS1,6. 0 OR 6ý- a1- 6 % 3 0.s-0% R(? 6 L 7 IT, 0ois6*IZo-Or 6 0oS-Is- 01A oto,0 I-LBO A~kb.1e6v*"51o$.tL- 0i 7 I~ 12~ __________ _______ Controlinformation: Acceptance criteria Summary of test endpoints:
%Mortality: " I <20% 7-day LC5 0 !1 o*l.
Average weight per initial larvae: ,1 te " NOEC 7.7.6% Average weight per surviving larvae: 0 .1 %, I > 0.25 mg/larvae LOEC A,1, ChV IC 25 [ *>*IcW7O Page 27 of 107
6 Page 2 of 6 0 J'ETS
£nwIw IlEOtI fln~SOiu bT% K Species: Pimephales promelas Date: -%'.- ooI Client: TVA / Sequoyah Nuclear Plant - Non-treated Survival and Growth Data Day CONTROL 11.3% 22.6%
A B C D E F G H I J K L 0i 0/((0 I /0 (0 /0 to to /0 /0 to 10 1 (00 I6 )0 t 0 /0 /0 /0 /0 /( to 2 10t/Q to IO to / to It to 4 )a 10 / 0 10 /0o /0 tO /0 to 1D 5ID oIb 10 /i 1b /0 /0 /0 /0 /0 6 toID IQ) to 11() _(3 to to cI toI /0 7 It I0C O t(0 (0 q0 (0 0t 10 at " 10 A = Pan weight (mg) liAt Tray color code:: 1it j 13 .15 1;A0 K so 11.40 5,69 j. 5o .11 ISý.13 13-.4 Analyst: fn,l r ""-") Date: 0 5"- _ __-_ _ __ B = Pan + Larvae weight Analyst: NA..IO Date: r-05 - C = Larvae weight (mg)
=B-A Weight per initial number N
of larvae (mg)
= C / Initial number of larvae Q~) A I, C" t,.
Average :Percent weight per reduction initial from control c.1?- 1.V7." 0.1 s. number of (%) larvae (ag) Comment codes: c = clear, d =dead, fg = fungus, k = killed, m = missing, sk sick, sm = unusually small, lg unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 28 of 107
Page 3 of 6 E TS Species: Pimephalespromelas Date: 05-12-cA Client: TVA / Sequoyah Nuclear Plant - Non-treated Survival and Growth Data Day 45.2% 72.6% 100% M N 0 P Q R S T U V W X 00"_ _ 1(/0 (0 /10 1 /0 10 10 103/0 to I U I IO t C) to to tOC) to to /O 2 C)I1 t(o /to 10 lo I0o 6 /0 to, I ) 3 10 11(3 (0 I10 10 0* 10 0 /03 I0 4 1 l./0 10 Io 0 10 to /0 t0 to to 50 (0 IC) /O /0 0* IO /O 10 q 10 tO 6 Io 10 10 10 to
/t Itb to / 9 i) 10 7 ISM to 10 T to 16 /) cl /0 t(
A = Pan weight (mg) Tray color code:: P!l Y3rJ3L l.S Analyst: ,ALlt r. ,.3.- 0 I4.o 3." __-0_ .gt . jjg1 I4: l3 Date: 5- '- _ B = Pan + Larvae weight (mg) aD,3ý o,.81 X 08 P.S"7 A,03- A ý, 0 X..33 U. 10 Analyst: 09P - "" Date: i . A.Q-.
- C = Larvae weight (mg)
= B- A 6 . 6,
- q t.0"U bfr(1 4a6 ,. 6 5* 6. 5 L, .6 *,.5ýo 6,-0-5 " .t (,*. %41 Weight per initial numberf', A 0 of larvae (mg) 1\' '
= C / Initial num ber of larvae \ - N"
* \. . 4"V,
-w -. - - -- t- -
Average Percent weight per reduction -17 initial from control 1 .* number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk - sick, sm = unusually small, lg = unusually large, d&r - decanted and returned, w = wounded. Calculations and data reviewed: A Comments: Page 29 of 107
Page 4 of 6 lmETS E-wIM~nmp ft. blg soU~ I , Species: Pimephalespromelas Date: OS-i'2-0 Client: TVA / Sequoyah Nuclear Plant - Non-treated 7-Survival and Growth Data Day 100% Intake Y Z AA BB 0 0 /0 ( t0 "/0 _______ /L /0 /0 /0 2 10 (10I 3 4 qL4A1 I 1,~ 5 3 V'jF4 d~ 1-6 AF 7 1AFi A = Pan weight (mg), Tray color code:: ' ili.* I'-jti. - i.'t Analyst: _U "- _ Date: b6 '- 1-8 41 B = Pan + Larvae weight (rag) lSq *.l q* ql Analyst: _ ) I Date: D57 -___- __ C = Larvae weight (mag)
=B-A Weight per initial number of larvae (mg) q t% *"#D 0 \
= C / Initial number of larvae Average Percent weight per reduction initial from control o. +1 .2 7 number of (%)
larvae (nmg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comm ents: , A* . -vt t3 - Ae I.Fikde #"M INT AYP_ b OI e - Mt MTi . NC.. I~,
-_ba4 R~J6U9 feIse~ejT- ný IeAtý LAOvAE. 4U 1 Page 30 of 107
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated
-- i May 12-19, 2009 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
- Environmental Testing Solutions, Inc.
Project number: 5444 Reveiwed by: - Not for Compliance Assessment, Internal Laboratory QC ___ raPplicau
%) Initialt nmbetof Finial nuimber of A -Fan wright B Psn + La l L i cght (mg) wrn Weight/Swrviving M s.a eightl Cafdentao rada a Weigillt niitial taiber Mlea ns airvivi Mcnarweight I t of Pe rcet eadon hr-la uae la rae (rg) waigit(mg) -A-B lrolanaermeJ 8,rsivinganmreeef 'ai' ' - dOfambeatia g.Initiual hlan j 1%) .beraof variat u..oni. rra ( %n )
lavat (J." heva (tR) 1 A 10 t0 14.87 22.17 . 7.30 0.730 0.730 Control a C t0 10 t0 t0 13 86 15.01 20.70 23.96 6.84 8.95 0.684 0.895 0768 11.8 0.694 0.895 100.0 0.768 11.8 Not applicable D 10 10 12,40 20.01 7.61 0.761 0,761 E 10 t0 14.50 22.03 7.53 0.753 0.753 11.3% F 10 9 1370 20.25 6.55 0.728 0.730 2.2 0.655 97.5 0.712 5.8 7.2 G 10 10 13.68 20.91 7.23 0.723 0.723 IL 10 10 14.50 21.67 7.17 0.717 0.717 1 10 t0 1391 20.45 6.54 0,654 0.654 22.6% 1o 9 13.27 20.50 7.23 0.803 0.723 9.8 0.723 97.5 0.703 6.9 8.5 K 10 t0 15.13 21.86 6.73 0.673 0.673 L 10 10 13.90 21.50 7.60 0.760 . 0.760 M 10 10 . 13.89 20.35 6A6 0.646 0.646 45.2% N 10 I10 13.97 20.81 6.84 0.684 0672 4.5 0.684 9.5 0.654 3.1 14.1 0 10 9 13.70 20.08 6.38 0.709 0.638 P 10 10 14.08 20:57 6.49 0.6.49 0.649 Q _20.37 0 .to0 13.72 6.65 0.665 0.665 72R 10 10 1575 22.07 6.32 0.632 4.3 00.639
&632 100.0 0.639 43 16.7 S _0 10 14.24 20.80 6.56 0.656 0.656 T 0" 10 14,28 203) 6.03 0,603 0.603 U to 10 )4.94 22.17 7.23 0.723 0.723 100% W 09,1 10 6.04 0.671 0.684 5.2 . 0604 97.5 0.667 , 8.2 13.1 10 1433 2333. 7.00 0.700 0.700 X 10 10 )369 20A0 6.41 0.641 0.641 Y 1O 3 13.79 15.91 2.12 0.707 0,212 100% Intake _ __0 4 14!14 16.99 2.85 0.71 0.764 8.3 085.50.0 0.390 43.2 49.2 AA 6 14.85 19.81 496 0.827 0.496 8B 10_ 7 13.49 19.16 5,67 0810 N0.567 Outfall 101: MSD = Minimum Significant Difference Daunnett's MSD value: 0.0890 PMSD = Percent Minimum Significant Difference 11.6 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
PMSD: Itaake: Dunnett's MSD value: 0.1860 Lower PMSD bound determined by USEPA (l0th percentile) = 12%. PMSD: 24.2 Upper PMSD bound determined by USEPA (90th percentile) = 30%. Lower and upper PMSD bouinds ware determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET lnterlaboratory Variability Study (USEPA. 2001 a USEPA, 2001b). 00 USEPA. 2001 a, 2 1b. Final Report: Interlaboratory Variability Study ofEPAShort-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-04 and EPA-821-B-0I-005. US Environmental Protection Agency, Cincinnati, OH
TVA / Sequoyah Nuclear Plant, Outfall 101 0 S Non-treated I
"ETSEnvironmental Testing Solutions, Inc.
May 12-19, 2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: TVA/ Sequoyah Nuclear Plant, Outfall 101 End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. . Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-% 1 2 3 4 D-Control 0.7300 0.6840 0.8950 0.7610 11.3 0.7530 0.6550 0.7230 .0.7170 22.6 0.6540 0.7230 0.6730 0.7600 45.2 0.6460 0.6840 0.6380 0.6490 72.6 0.6650 0.6320 0.6560 0.6030 100 0.7230 0.6040 0.7000 0.6410 Intake 0.2120 0.2850 0.4960 0.5670 Transform: Untransformed I-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7675 1.0000 0.7675 0.6840 0.8950 11.817 A4 0.7675 1.0000 11.3 0.7120 0.9277 0.7120 0.6550 0.7530 5.777 4 1.502 2.410 0.0890 0.7120 0.9277 22.6 0.7025 0.9153 0.7025 0.6540 0.7600 .6.851 4 1.760 2.410 0.0890 0.7025 0.9153
*45.2 0.6543 . 0.8524 0.6543 0.6380 0.6840 . 3.113 4 3.066 2,410 0.0890 0.6543 0.8524
*72.6 0.6390 0.8326 0.6390 0.6030 0.6650 4.343 4 3.479 2.410 0.0890 0.6530 0.8508
*100 0.6670 0.8691 0.6670 0,6040 0.7230 8.152 4 2.721 2.410 0.0890 0.6530 0.8508 Intake 0.3900 0.5081 0,3900 0.2120 0.5670 43.230 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97087646 0.884 0.63537989 1.21808683 Bartlett's Test indicates equal variances (p = 0.23) 6.92620468 . 15.0862722 Hypothesis Test (I-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 22.6 45.2 31.9612265 4.42477876 0.08902763 0.11599692 0.00884297 0.00272926 0.0292745 5, 18' Treatments vs D-Control Linear Interpolation (200 Resamples)
Point % SD 95% CL(Exp) . Skew IC05* 7.813 ICI0 28.104 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100
- indicates IC estimate less than the lowest concentration sqnIO1_05-12-O9data
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake 0 Non-treated 0 -4 May 12-19, 2009 S 0 0 Statistical Analyses Environmental Testing Solutions, Inc.
-Larval Fish Growth and Survival Test-7 Day Survival Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-82 I-R-02-013 Test Species: PP-Pimephales promelas Comments:
Conc-% 1 2 3 4 D-Control 1.0000 1.0000 1,0000 1.0000 11.3 1.0000 0.9000 1.0000 1.0000 22.6 1.0000 0.9000 1.0000 1.0000 45.2 1.0000 1.0000 0.9000 1.0000 72.6 1.0000 1.0000 1.0000 1.0000 100 1.0000 .0.9000 1.0000 1.0000 Intake 0.3000 0.4000 0.6000 0.7000 Transform: Arcsin Square Root I-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD "D-Control 1.0000 1.0000 1.4120 1.4120 1,4120 0.000 4 11.3 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 22.6 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 45.2. 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 72.6 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 100 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4
*Intake 0.5000 0.5000 0.7854 0.5796 0.9912 23.814 4 6.701 2.353 0.2201 Auxiliary Tests Statistic Critical Skew Kurt Shapi'ro-Wilk's Test indicates normal distribution (p > 0.01) 0.93208891 0.749 3.38354E-16 0.88161324 Equality of variance cannot be confirmed Hypothesis Test (I-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Heteroscedastic~t Test indicates significant differences 0.11179766 0.11466427 0.78530011 0.01749093 5.4E-04 1,6 Treatments vs D-Control sqnIO0_O5-12-O9data
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated May 12-19,2009 E Statistical Analyses '., Environmental Testing Solutions, Inc. Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWC1-R-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-% 1 2 3 4 D-Control 0.7300 0.6840 0.8950 0.7610 11.3 0.7530 0.6550 0.7230 0.7170 22.6 0.6540 0.7230 0.6730 0.7600 45.2 0.6460 0.6840 0.6380 0.6490 72.6 0.6650 0.6320 .0.6560 0.6030 100 0.7230 0.6040 0.7000 0.6410 Intake 0.2120 0.2850 0.4960 0.5670 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.7675 1.0000 0.7675 0.6.840 0.8950 11.817 4 11.3 0.7120 0.9277 0.7120 0.6550 0.7530 5.777 4 22.6 0.7025 0.9153 0.7025 0.6540 0.7600 6.851 4 45.2 0.6543 0.8524 0.6543 0.6380 0.6840 3.113 4 72.6 0.6390 0.8326 0.6390 0.6030 0.6650 4.343 4 100 0.6670 0.8691 0.6670 0.6040 0.7230 8.152 4
*Intake 0.3900 0.5081 0.3900. 0.2120 0.5670 43.230 4 3.944 1.943 0.1860 Auxiliary Tests Statistic Critical . Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 001) 0.94991392 0.749 0.13317371 -1.3590747 F-Test indicates equal variances (p 0.34) 3.45560646 47.4672279 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.18600391 0.2423.5037 0.2850125- 0.01832517 0.00759199 1,6 Treatments vs D-Control sqn101_O5-12-O9data
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 12-19, 2009 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Environmental Testing Solutions, Inc. Project number: 5444 Reviewed by: Concentration Parameter Da 0 Day 1 Da Da 3 Da4Da 1_.Da 54 6 ________ ________________ Initial Final InitialInitia IFinal Initial
- Fnl Initial Final Initial IFinal IInitial Final inl Initial IFinal pH (SUJ) 7.32 7.28 7.601 7.15 7.65 713 7.58 7.30 7.601 7.441 7.43 7.38 7.661 7.04 DO (mg/L) . 7.5 .7.4 7.61 7.4 7.4 7.5 7.7 7.5 7.81 7.51 7.6 7.3 7T41 6.9 Conductivity (pmhos/cm) 317 302 326 Control Alkalinity (mg/L CaCO 3) 61 616 Hardness (mg/L CaCO 3) 88 Temperature CC) 8 8 8 24.5 24.7 24.5 24.7 24.5 24.7 245 247 24.5 24.8 24.6 PH (SIJ) 7.47 7.21 7.40 7.22 7.39 7.121 7.26 7.30 741 7.36 7:50 7.33 7.42 7.01 DO (mg/L) 75 73 76 72 7.6 7.3 7.8 7:5 80 74 77 7.4 75 6.9 11.3% Conductivity (pmhos/cm) 293 292 284 298 304 300 306 Temperature (C) 24.8 24.9 24.8 24.5 24.7 24.5 24.7 24.7 247 242 247 24.5 24.8 24.6 PH (SU) 747 7.18 741 715 7.37 7.09 726 7.30 740 734 749 7.33 7.41 7.03 2.% DO( L) 7.5 7.1 7.71 7.1 7.6 7.3 7.81 7.5 8.1. 7.41 7.7 .7.4 7.5 6.8 22.6% Conductivity (pmhostcm) 28 277 268 279 286 280 287 TemperatureC) 248 251 248 24.3 247 24.3 247 24.7 248 242 24.8 24.4 248 24.5 pH(SU) 747 719 741 715 731 7.09 723 7.30 736 735 7.45 7.28 7.37 6.99 DO(mg/L) 77 71 77 71 76 72 78 7.4 80 74 7.7 7.4 7.6 6.8 45.2% Conductivity (pmhos/cm) 247 243 234M 01947 243 246 Temprerature C) 248 248 248 247 . 247 242 247 248 249 245 248 24.4 24.8 24.5 PH (SU) 7.46 7.12 7.35 7.12 7.27 7.09 7.18 7.29 7.30 7.34 7.42 7.23 7.32 6.97 DO (m L) 7.7 7.2 7.7 7.1 7.7 7.3 7.9 7.5 7.8 7.4 7.7 7.4 7.8 6.8 7./0 Conductivity (junhos/cm). 205 202' _____ _189196___ 196 199
________Temperature ('C) 24.8 24..8 24.9 24.5 24.7 24.6 24.7 24.6 24.9 24.5 24.8 24.4 24.9 24.6 pH (SU) 7.391 7.131 7.30 7.12 7.191 7.041 7.141 7.231 7.24 7.23 7.361 7.241 7.26 6..951 DO (mgL) 7.71 7.11 7.7, 7.1. 7.71 7.51 7.81 7.41 . 7.8, 7.3 7.71 7.31 7.81 6.86 Conductivity (pinhos/m) 1145 214 100% Alkalinity (mg/L CaCO3) 57 50 Hardness (mg/L CaCO3) 6 60 Total Residual Chlorine (mg1L) <0.10 <01 Temperature ('C) 24.8 24.9 24.9 24.5 24.71 24.4. 24.7 24.6 25.0 24.6 24.9 24.6 24.9 24.6 pH (SUJ) . 7.39 7.15 7.3011 7.05 7.171 7.011 7.111 7.24 7.221 7.25 7.341 7.231 7.25
. 69 DO (mg/L) . 7.8 7.2 7.71 .7.1 7.81 7.41 7.9 7.5 7.81 7.31 7.71 7.41 7.86.
Conductivity (I~mhos/cm) 153 143 48 .7 100% intake Alkalinity (ng/L CaCO 3) 54 Hardness (nsg/L CaCO3) (6 Total Residual Chlorine (mg/L) <0. <0.10 _Temperature ('C) 24 24.9 24.6 24.9 24.6 24.6 24.7 25.11. 24.5 24.8 24.4 24.9 24.5
Page 5 of 6 EDTS Species: Pimephalespromelas Date: o5-aL-00% Client: TVA/ Sequoyah Nuclear Plant - Non-treated Daily Chemistry: Day Concentration Parameter CONTROL pH (S.U.) -4.32 :51 Conductivity T) (j-mhos/cm) Alkalinity (mg CaCO3/L) , Hardness (mg CaCO3VL) ________ Temperature ('C) j. ~ i.- ~ L~S -A1 'i. pH (S.U.) Y 11.3% DO (mg[L) Conductivity 3 (jimhos/cm) Temperature (°C) L.q. k [ iA., * "i pH (S.U.)3.t'T34 22.6% DO (m.gf) 7-7 . Conductivity (_Wmnhos/cm) 0 Temperature (°C) t.4. , "..- * .- "L ,/, pH (S.U.) L-4 41 45.2% DO (mg/b) 11 Conductivity (j~imos/crn) _________Temperature ('C) i~' ___ 1 1 t pH (S.U.) T______ 72.6% DO (mg/b) Conductivity _ _ _ __.. 9 (jimhoslcm) Temperature ('C) 1I - -IAq1 PH (S.U.)3 100% DO (mgf/ *-4) Conductivity (pmnhos/cm) Alkalinity (mg CaCO 3/L) Hardness . (mg CaCo nL) 6oq 3 TR chlorine (mgfL) .4.001 __________Temperature ('C) i' ?L . i.*.I PH (S.U.) 100% Intake DO (mg/b) 7 Conductivity) Alkalinity (mg CaCO3 /L) S Hardness (mg CaCOJ/L) TR chlorine (mgIL) <4.6 10 .0. ________Temperature ('C) 2-1. zN 0 z 9 N Initial Final I Initial 11 Final I Initial 11 Final Page 36 of 107
i~TS Page 6 of 6
~ En~,o MT~5ng5dw~ h,~.
Species: Pimephales promelas Date: 05- a-001 Client: TVA / Sequoyah Nuclear Plant - Non-treated I I Dav I ID Analyst I U I- 3 .I 4 - 5 I 6 I Concen- Parameter traili I _________ CONTROL pH (S.U.) 1.(00. 4ý+3 1 t-.55 1 4-466I DO (m-/L) 4 - - "_3 18 I Conductivity 33k 3*L-(4mhos/cm) Alkalinity B -L (mg CaCO 3/L) Hardness lk_-4 (me CaCO,/L) -3
*-4 Temuerature (MC) 4
- zq, ,A*S I 4..1 1 9 - - 4~-ll DH (S.U.) ýim ir- -4,3(o 1. -2051 :f33 1-.'I I "I~L 11.3% DO (maL) ~a~o Iii 8.0 1114 11-.4 1 -45 1V A Conductivity I 309 ot 3oco (Anib 0 M)
Temperature (°C) LA. I -U .1 0.-1 P"WM, A 1 P". M, - a - -.-..-. l = *" DH (S.U.) 4 ~ 0 1 ;7Z79 -MI 9 1)3,4 1"-,4q II _-4.33 1 I L-1 II -. O-H I I 22.6% DO (m,/L) Conductivity (limbos/m) Te..n.erat.... (0 -C I T emperature ('Q pH (S.U.) 1.?0 I ~47L~L 3 I~Y[aS I i~* II ~.2iL~A~J 45.2% I DO (mg/L) Conductivity (grmhos/cm) Temperature ('Q I (C 4-Itur i Iql° 2_q 7I, ,* -- I i pH (S.U.) I
~~7I *i.2q I ~l-7 4~A~)1 ~4 I ~I4d~ II 1.~-3 I i.~; IK~IAIJ 72.6% DO (mg/L) 2~7 1_4j- I A4 -4,93L Conductivity (Amhos/cm) tC(4 199 Temperature ('C) -2A. t LqA %qA -L4 . LA DH (S.U.) .1.1 7-iaI '3 Ih1~ ~ A 14 i -4.tAL I! O I 100% DO (mi/L) 4 -
Conductivity Lcm) Alkalinity Hardness (Mg CaC03/L) I TR Chlorine (mgfL) Temperature (°C) N - ~ -~ 14- _ 14.9 VA.6 i .,(I t., 9 I WH (S.U.) I 4,1/ 11 7.I*IZ I t770 1 X3 I1 .'1 IlI 1q1 -. -- . I 31I I -- II U .
,GA, I I I 100% DO (mr/L) I* -'-~-.-
'I*:* II .
Intake Conductivity Alkalinity (mg CaCO3/L) I +' Hardness (rag CaCO3/L) TR chlorine (mg/L) Temperature ('C) 0-,g II . 1.Lt I ~ II ~ I IP 't ; It 11 T r"'i, - F Initial Final Initial Final I Initial cR - N 17 F-i n7l Page 37 of 107
a 0 Page I of 7
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Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphniadubia Client: TVA County: Hamilton Facility: Sequoyah Nuclear Plant Treatment: Non-treated NPDES #: TN 0026450 Outfall: 101 Project #: .;,A'A Dilutionpreparationinformation: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism source information: Test information:
.Organism age: I< 24-hours old Randomizing template color: istoe_
Date and times organisms were born Os- I'L.-cf&\ o01-b -To M-AO. Incubator number and shelf between: location: Culture board: O5-06,-C A Replicate number: 1 12 13 4 15 16 17 a 9 10 yWVT batch: Culture board cup number: 3' 5 ID SI t'\-I L 2 "LV "LS OITL-CA Transferbowl information: pH = \.q'6 SU Temperaturen *A.. C Selenastrum batch: O TM Daily renewalinformation: Day Date Test initiation, MHSW Sample numbers Analyst renewal, or batch used used _termination time 0 1 *C,-6,i-o* 0I5,O OS-0o-ova q A o\o cA ,--02. 2 -- Oo -c -O O OOS&50%-0CJ t
"3 0, -so -0ý i 1 )St .0V -
4 ____-__ 0Do OS - %- OOct o . O-t OOs 5oS-1'*-* oL'-Arr) " OS- 2-D9 0o0,o o1-'_
- t. Ad E7 c- 101-)
Control information: 1 2 Acceptance criteria Summary of test endpoints:
% of Male Adults: C) Q7*7. s 20% 7-day LC50 ) I ,
% Adults having 3 rd Broods: /o067. 1007. >80% NOEC I D07
% Mortality: 07. 07.' < 20% LOEC > I067.
Mean Offspring/Female: 323.0 ,5!,. t 15.0 offspring/femalc ChV > 1067.
%CV: L "7 <40.0%
13%7 IC25 > Io7.T Page 38 of 107
- Page 2 of 7 Species: Ceriodaphniadubia Client: Sequoyah Nuclear Plant - Non-treated Date: Os- *--ot CONTROL -1 Surv'ival andReproductionData Replicate number Da*'
I pr-duced Young produced Adult mortality Young produced 1 2
-on 0.0_
C) 3 o) 4 C-5 I.___ 6 7 8 9 C) 10 Adult mortality ._ 4 Young produced - I-A
-A Adult mortality L~x _ I_'-I '1-I k1
- 1 l t, l° t Young produced I_ Ib'A TSIb __ *\ -, t 7'[Young produced Adult mortality I ____
.IL- ____ ____ \_\____x \ . .'VIL
..- I ,_
6 Adult mortality Young (L= e D-= dead) producedc - S 1 o Y unro p' ducds C,) (D 0- 0 0. 0. 0, 0, 0 q, Concentration:
% Mortality:_Attt\.
Mean Offspring/Female: a3. C oNC: 11.3% Survival and Reproduction Data Replicate number Day' TtI Young produced 1 0 2 3O 3-31 4 5 6 I 0A 7 I 1 8 9 10 l ° °C11)* F Adult mortality L- -- . I--t. . I. L ,__ 2 ,Young produced* oj 6 Adult mortality Young (L live 3 YoungCproduced produced Adult mortality dead) Q) . c
-"=
0 c) oncet Lo I rtC on:1 Qi ._.. c0 . I 4 Young produced Sur S
- l a nd\eprducionDa
_ mortality _na Adult `L- . .. q - I Adult mortalityiv D,=-ad \- \- Concentration:
% Mortality: .
Mean Offspring/Female: I% Reduction from Control: I- 1.17.1 . Page 39 of 107
Page 3 of 7 T a a*, n *nn l T~ n0****Ir Species: Ceriodaphniadubia Client: Seauovah Nuclear Plant - Non-treated Date: OS- LI-C) CONC: 22.6% Survival andReproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced 0) o' 0 0: o c) O C)* Adult mortality % -L I k L.__ L_. 2 Young produced ...... 0l 0 LD c[ o Adult mortality II *t--' ,- -- ,-I t, [n 1 1I o b 0a ol 0 Young produced Adult mortality I - -4 ' I -I -[-V - 4 II Young produced ____ __IL ___ ___ 'Is 1&_ Adult mortality \I-A I,- i.- , I~[~I~I 4,-- I Aut mortality I A_____ _[~ ~ 6 Young produced[ C) o *Cl)l~ CýI rAdult mortality I _ ___[l __ __ _--I __ _l-_ 7 Young produced I t,\I 19 I'S 1 S IS Total young produced ". l I32- Sb- -6.S -6 I6 2"- Final Adult Mortality .- ._ *- k. .. _ Note: Adult mortality (L live, D = dead) Concentration:
% Mortality:
Mean Offspring/Female: 13g.1
% Reduction from Control: "
CONe: 45.2% Survival andReproduction Data Replicate number Day 1i- 2 3 .4 5 6 7 8 9 10 1 I Young produced Adult 0 0.
-otlt
\_
0
-\-I 0 0
- ,- I 0 0ý- I 0 L- 0 Adult mortality . . . ... _ 1 k ... L L j.
23 Young produced CC ICDI- ___{ Adult mortality x 1 43 Young produced Adult mortality . Young produced IS___ [-S[ ____6 ___ ___ _ 5 f Adult mortality [Young Adult produced mortality _ _._
- I_
0_ 0_ [\- k.-. C_
.K 0_
L... _______ C___ I,... 6 Young produced 1 produced young "5o{ 3A 3 0*1" S [%I3.* 3 3,4 "% a, Total youn prdcd=-
'~ S ~ q~
Final Adult Mortality 1 .. 3
""_ ._ . I~ _
Note: Adult mortality (I. = live, D dead) Concentration:
% Mortality: T7 Mean Offspring/Female: 3 n' .S
% Reduction from Control: -\.S7.
Page 40 of 107
l In Page 4 of 7
-, Enlu--u-nalIf --
otlbs.] Species: Ceriodaphniadubia Client: Sequoyah Nuclear Plant - Non-treated Date: o5-%L-QCk CONC: 72.6% Survival andReproduction Data Replicate number Day,1 1 2 3 4 5 6 7 8 9 10 I Young produced _._ 0 c 0 2[3 Young 0Q 00I C3 .3 You produced produc Adult mortality Adult mortality produced SYoung L- k.. 4 I__0 C '--I 0~~ 0C_
- 0_
q I .. o100 Adult mortality j.. ... _I, %._ ._ j 5 Young produced [ ku Adult mortality _I 1 IL k.-...- =_= 7 , Young produced 's k[ ] ' \. 5 IA 11 I1 \o Total young produced b' ". Z"' 2 Final Adult Mortality Note:
- Adult mortality (L = live, D = dead) i 1 L ' . L* '-
Concentration:
% Mortality: ONY.
Mean Offspring/Female: aq . I
% Reduction from Control: -5.37.
CONC: 100% Survival andReproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1I Young produced Q I fi 0 0Q o.. i 1 ( Adult mortality U L.- L.. li. L .. L... [Young produced j ) 10c Adult mortality iI °- 3 Young produced I~t .. ci 01 0S 0 'A kI. Adult mortality _ - 1 ... . U._
- I..[U 5 Young produced SA I -b 2L Adult mortality - I _.-.1 _. I __'-I '. 1_ ) I ' ._I 6 Young produced_1 01 0 01I0 0 lo 5o7o
___ Adult mortality j \ý-I -
~- .
~ I~ _ I I- ..-- I I j . L-..-
7 Young produced ~ I~ j ' ~ ' ~~c ~ I Total young produced Iý ,S& z I, B'*"- 31A 3S 3A .31 Final Adult Mortality L..
- T _ .... 1c?_. ...-
Note: Adult mortality (L = live, D dead) Concentration:
% Mortality: 07.
Mean Offspring/Female: 31.i
% Reduction from Control: - '-
Page 41 of 107
Page 5 of 7
. EnvI-nMWmAlTýtlngoIVdI-1-*
Species: Ceriodaphniadubia Client: Sequoyah Nuclear Plant - Non-treated Date: oC- I*.L-O CONTROL-2 Survival and ReproductionData
.... _Replicate number Day , 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 C) 0 "0 0 . C Adult mortality %-_.. __. k,..,
-. L..
3 Young produced Adult mortality Adul moraliy i, I- - I\- -- ý - -- 0 I° I-k-C aJ 1 5 Young produced Adult mortality Adult mortality _ .. _1 13.. =3 )S-._ 11 lLJ [ 1 ... q_.._ -..
° 1...n C_ C_
6 Young produced Adult mortality ___.__ [.L L
' . 13.
13l 1.CA O 4 4 i.._ 6 I Young produced \[
- I * *.cia* \* I I-' Ic* [
7 Young produced 11 o tk -1 1 c I'A 1-1 Total young produced 3! 3S *A 3
- k Final Adult Mortality I I..... v.-.... _ .- X '-.. '... ..
Note: Adult mortality (L live, D = dead) Concentration:
% Mortality: I "L Mean Offsprin Temale: .
CONC: 100% Intake Survival and ReproductionData Replicate number Day,_, 1 2 3 4 5 6 7 8 9 10 2 Young produced I0.-. G 0 I Adult mortality .- __ L .- ___ - - '. , '. I ._ 3 Young produced ___ 0 j0 02- _. ._ 0f~Iie_ Adult mortality F L- ___ 4 Young produced C) _.. _ Adult mortality ______ ____________.______
\_____ ____________ _____
Adult mortality _ 6 Young produced SQ 'Ao- 1 qc S S I1 0A Adult mortality 6 ]Young produced ) 0C o 0 5 Young produced I*'-- Adult mortality -L V\-*I**I* Iqz~.. 1*-IO17- 13,.I( HQ ,.[*_{*. 'CI.. 13 1___
- 13 .
7 Y~oung produced I- 7\ iglo Cw* -L_( a is 12 lot, 1--1 Total young produced [ ' 3 -" 3o 31 3' I Final Adult Mortality %-1...- ... '.. "l.. . Note:' Adult mortality (L = live, D = dead) Concentration:
% Mortality::)T.
Mean Offspring/Female: 1,36.
% Reduction from Control: 1 I- i 27.° Page 42 of 107
S TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated C 0 -1 0 0 FTSz Environmenta Testing Solutions, Inc. Control-I May 12-19 2009 Verification of CeriodaphniaReproduction Totals 72.6% Day Re licate number - - Total Re plicate number Day 1 2 3 1 4 5 6 7 8 1 Total 1y 2" 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 5 5 5 5 4 4 45 4 6 4 5 5 5 4 4 4 4 4 45 5 13 13 13 12 13 I1 14 12 12 .12 125 5 13 15 15 13 12 13 13 13 14 13 134 6 0 0 0' 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 17 15 18 16 16 15 17 15 15 160 7 18 18 16 14 18 15 14 17 16 16 162 Total 33 34 33 34 34 32 34 34 31 31 330 Total 37 37 36 32 35 32 31 34 34 33 .341 11.3% 100% Day Replicate number Total Day 1 2 3 4Replicate 5 number 6 Total Daa1 2 3 4 5 6 7 8 1 9 10 1 7 8 "9"T 10 1 0 0 0 0 0a 0 0 a0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0. 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 5 5 6 4 4 4 4 4 45 4 5 6 4 5 5 5 4 6 6 4 50 5 12. 14 12 13 13 13 13 14 13 13 130 5 15 14 12 14 13 14 13 13 13 12 133 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 17 15 14 17 16 16 16 16 15 159 7 19 18 16 17 16 14 17 16 15 15 163 Total 33 36 32 32 36 33 33 34 33 32 334 Total 39 38 32 36 34 33 34 35 34 31 346 22.6% Control-2 Replicate number Total Day 1 2 3 4 5 6 17 8 9 10 Day
- 0 0 1 2 3 ,4Re plicate 5 number 6 7 8 91" 10 Total 0 0 0 O1 0 0 0 1 0 0 1 0 -0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0- 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 -0 0 0 0 0 0 4 5 4 6 6 4 4 5 4 5 5 48 4 4 4 4 4 5 4 5 4 4 4 42
- 5. 14 13 13 .14 .11 14 12 13 13 13 130 5 14 12 13 13 13 13 12 10 14 12 126 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 19 15 16 17 15 18 17 15 15 163 7 15 19 16 18 16 16 14 17 15 17 163 Total 35 36 34 36 32 33 35 34 33 33 341 Total 33 35 33 35 34 33 31 31 33 33 331 45.2% 100% Intake Da Re licate number ay 1 2 3 4 5 6 7 8 9 10 Total Day Re licate number Total 1
1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 .0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 5 5 5 5 5 5 4 4 47 4 5 4 6 4 4 5 5 5 5 4 47 5 15 12 12 13 14 14 12" 130 5 15 12 14 14 12 13 14 13 13 13 133 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 18 14 17 17 16 15 15 15 15 158 7 19 19 21 18 19 20 18 18 19 17 188 Total 36 34 31 35 35 33 33 34 33 31 335 Total 39 35 41 36 35 38 37 36 37 34 368
-v TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 12-19, 2009 Ceriodaphniadubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control
- Environmental.Testing Solutions, Inc.
Verification of Data Entry, Calculations, and Statistical Analyses Project number: 5444 Reveiwed by: Concentration Replicate number Survival Average reproduction Coeffcien(of Percent reduction from 3 4 5 6 7 8 9 10 (%) (offspring/female) va"at"on (%) pooled controls (%) (%) 1 2 Control- 1 33 34 33 34 34 32 34 34 31 31 100 33.0 3.8 Not applicable 11.3% 33 36 32 32 36 33 33 34 33 32 100 33.4 4.5 -1.2 22.6% 35 36 34 36 32 33 35 34 33 33 100 34.1 4.0 -3.3 45.2%/o 36 34 31 35 35 33 33 34 33 31 100 33.5 4.9 -1.5 72.6% 37 37 36 32 35 32 31 34 34 33 100 34.1 6.3 -3.3 100% 39 38 32 36 34 33 34 35 34 31 100 34.6 7.2 -4.8 Control - 2 33 35 33 35 34 33 31 31 33 33 100 33.1 4.1 Not applicable 100% Intake 39 35 41 36 35 38 37 36 37 34 100 36;8 5.7 -11.2 Outfall 101: MSD = Minimum Significant Difference Dunnett's MSD value: 1.831 PMSD = Percent Minimum Significant Difference PMSD: 5.5 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Intakce Dunnett's MSD value: 1.374 Lower PMSD bound determined by USEPA (IOt percentile) = 13%. PMSD: 4.2 Upper PMSD bound determined.by USEPA (90' percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001 b), USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix_ EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
TVA / Sequoyah Nuclear Plant, Outfall 101 (11 0q Non-treated --..1 o May 12-19, 2009 Statistical Analyses
'.) Environmental Testing Solutions0 inc.
Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:. 5/12/2009 Test ID: CdFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: :5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-82 I-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-% 1 2 3 4 5 6 7 8 9 " 10 Control- 1 33.000 34.000 33.000 .34.000 .34.000 32.000 34.000 34.000 31.000 31.000 Control-2 33.000 35.000 33.000 35.000 34.000 33.000 31.000 31.000 33.000 33.000 11.3 33.000 36.000 32.000 32.000 36.000 33.000 33.000 34.000 33.000 32.000 22.6 35.000 36.000 34.000 36.000 32.000 33.000 35.000 34.000 33.000 33.000 45.2 36.000 34.000 31.000 35.000 35.000 33.000 33.000 34.000 33.000 31.000 72.6 37.000 37.000 36.000 32.000 35.000 32.000 31.000 34.000 34.000 33.000 100 39.000 38.000 32.000 36.000 34.000 33.000 34.000 35.000 34.000 31.000 Intake 39.000 35.000 41.000 36.000 35.000 38.000 37.000 36.000 37.000 34.000 Transform: Untransformed I-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean Control-I 33.000 0.9970 33.000 31.000 34.000 3.779 10 33.783 1.0000 Control-2 33.100 1.0000 33.100 31.000 35.000 . 4.140 10 11.3 33.400 1.0091 33.400 32.000 36.000 4.508 10 -0.500 2.287 1.831 33.783 1.0000 22.6 34.100 1.0302 34.100 32.000 36.000 4.019 10 -1.374 2.287 1.831 33.783 1.0000 45.2 33.500 1.0121 33.500 -31.000 36.000 4.925 10 -0.624 2.287 1.831 33.783 1.0000 72.6 34.100 1.0302 34.100 31.000 37.000 6.252 10 -1.374 2.287 1.831 33.783 1.0000 100 34.600 1.0453 34.600 31.000 39.000 7.235 10 -1.998 2.287 1.831 33.783 1.0000 Intake 36.800 1.1118 36.800 34.000 41.000 5.700 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.653808415 1.035 0.254140335 -0.23607601 Bartlett's Test indicates equal variances (p = 0.26) 6.457315922 15.08627224 The control means are not significantly different (p = 0.87) 0.170664037 2.100922029 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV . TU . MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 1.83092058 0.055482442 3.416666667 3.205555556 0.389506936 5,54 Treatments vs Control-I Linear Interpolation (200 Resamples) Point % SD 95% CL Skew IC05 >100 ICI0 >100 ICI5 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqnlOlO5-12-O9data
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake 0 Non-treated -1 May 12-19, 2009 Statistical Analyses
- EnvironmentalTesting Solutions. Inc.
Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/12/2009 Test ID: CdFRCR Sample ID: TVA / Sequoyah Nuclear Plant, OutfaU 101 - Intake End Date: 5/19/2009 LUb ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-% 1 2 3 4 5 6 7 8 9 - 10 Control-I 33.000 34.000 33.000 34.000 34.000 32.000 34.000 34.000 31.000 31.000 Control-2 33.000 35.000 33.000 35.000 34.000 ' 33.000 31.000 31.000 33.000 33.000 11.3 33.000 36.000 32.000 32.000 36.000 33.000 33.000 34.000 33.000 32.000 22.6 35.000 36.000 34.000 36.000, 32.000 33.000 35.000 34.000 33.000 33.000ý 45.2 36.000 34.000 31.000 35.000 35.000 33.000 33.000 34.000 33.000 31.000 72.6 37.000 37.000 36.000 32.000 35.000 32.000 31.000 34.000 34.000 33.000 100 39.000 38.000 32.000 36.000 34.000 33.000 34.000 35.000 34.000 31.000 Intake 39.000 35.000 41.000 36.000 35.000 38.000 37.000 36.000 37.000 34.000 Transform: Untransformed I-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-I 33.000 0.9970 33.000 31.000 34.000 3.779 10 Control-2 33.100 1.0000 33.100 31.000 35.000 4.140 10 11.3 331400 1,0091 33.400 32.000 36.000 4.508 10 22.6 34.100 1.0302 34.100 32.000 36.000 4.019 10 45.2 33.500 1.0121 33.500 31.000 36.000 4.925 10 72.6 34.100 1.0302 34.100 31.000 37.000 6.252 10 100 341600 1.0453 34.600 31.000 39.000 7.235 10 Intake 36.800 1.1118 36.800 34.000 41.000 5.700 10 -4.670 1.734 1.374 Auxiliary Tests Statistic* Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0H01) 0.955419481 0.868 0.544660572 0.427540628 F-Test indicates equal variances (Ii = 0.22) 2.3431952 6.541089535 The control means are not significantly different (p = 0.87) 0.170664037 2.100922029 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.373940728 0.041508783 68.45 3.138888889 1.9E-04 I, 18 Treatments vs Control-2 sqn lOl_O5-12-O9data
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 12-19, 2009 Ceriodaphniadubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Project number; 5444 Environmental Testing Solutions, Inc. Reviewed by: Concentration Parameter Da 0 Da 1 Day 2 DaD Da 4 Da 5 Da _________________________ Initial Final Initial IFinal Initial Final Initial .Fnl Initial IFinal IInitial IFinal Initial Fia PH (SU 7.32 7.41 7.601 7.431 7.6 '5 7.21 7.58 7.1 7.601 7.471 7.431 7.40 7.66 73 DO (mgIL) 7-5 7.6 7.61 7.8 7.4 7.8 7.7 777.81 7.51 7.61 7.6 7.47. Conductivity (gmhos/cm) 3l7 302 326 3383 328 Control Alkalinity (mg/L CaCO 3) 61 61 Hardness (mg/L CaCO 3) 92 88 rTemperature ('C) 25.0 25.2 25.0 24. 24.7 24.8 24.7 24.8 24.7 25.0 24.8 8 73 722 7.44.4 pH (SU.) 7.47 7.4] 7.40 7.43 7.39 7.2 7.26 7.4 7.41 7.49 7.50 7.40 7.42 70 DO (mg/L) 7.5 7.5 7.6 7.7 7.6 7.8 8.0 7.5 7.7 7.6 7.5 4 11.3% Conductivity (Ismhos/cm) 293 292 284 298 304 300 Temperature (C) 25.0 25.2 25.0 24.9 24.7 24.8 25.0 24.9 24.8 25.0 24.7 25.0 24.8 0 H S).7.47 7.40 7.41 7.42 7.37 7.22 7.26 7.43 7.40 7.48 . 7.49 7.0 7.41 73 22.6% 2.% DO (m L). 7.5 7.6 7.71 7.7, 7.6 7.6 7.8 7.7 8.1 7.6 T7.76 7.51 7.4 Conductivity (Amhos/cm) 284 277 268 279 28 280 287 _Temperature (C) 25.0 25.0 25.1 24.9 24:7 24.8 25.0 25.2 24.8 24.8 24.7 24.8 pH (SUJ) 7.47 7.39 741 .421 7.31 7.22 7.23 7.42 7.36 7.48 7.45 7.387.3 3 45 0 DO (minL) 7.7 7.6 7.7 7.61 7.6 7.6 7.8 7.7 8.0767 7.6 7.6 746 Conductivity (jsmhos/cm) 247 243 234 243 247 243 46 rTemperature (C) 249 252 251 249 248 249 25.0 252 249 248 248 24.9 PH (SU) .7.46 7.38 7.35 7.37 7.27 7.22 7.18 7.38 7.30 7.42 7.42 7.57.32 7.32 DO (mg/L) .7 76 77 77 77 77 7.9 76 78 76 77 7.8 Conductivity (iimhos/cm) 205 202 189 19 196 196 199 Temperature ('C) 24.9 25.1 25.1 24.9 24.8 24.7 25.0 25.2 24.9 249 248 24.9 24.9 24.9 pH (SU) 7.39 7.34 730 733 7.19 7.20 .14 736 7.24 738 36 7.30 7.26 7.28 DO (mgL) 7.7 7.6 77 77 7.7 7.7 7.8 7.6 7.8 76 77 7.6 7.8 7.5 Conductivity (pmhos/cm) . 5 154 145 152 1461 100% Alkalinity (mg/L CaCO3) 65 57 50 Hardness (mg/L CaCO 3) . 62 60 Total Residual Chlorine (mL) <O. 1 0.10 _______Temperature ('C) . 24.9 25.1 25.11 24.8 24.81 24.7 25.01 25..0 25.01 24.81 24.81 24.91 24.91 24.91 pH (SU) 7.39 7.32 7.30 7.30 7.171 7.09 7.11 7.33 7.22 735 7.34 7.30 7.25 7.26 DO (mgIL) 7.8 7.6 7.71 7.6 . 7.81 7.71 7..9 7.6 7.81 .7.51 7.71 7.5 7.8 7.5 Conductivi mhs/cm 153 152 143 18 14514 100%-Intake Alkalinity (mg/L CaCO3) 57 54 52 Hardness (mg/L CaCO 3) 64 64 60 Total Residual(<0. Chlorine 10 ý <0,10 <0.10 _______Temperature (*C) 24.91 25.11 25.11 24.8 24.81 24.61 .2. 25.0 24.81 24.91 24.81 24.91 24.9 24..9
- Page 6 of 7 Species: Ceriodaphniadubia Date: 0c- i'-o4\
Client: Sequoyah Nuclear Plant - Non-treated Daily Chemistry: Day 0 ~ 12 Analyst ll A A e ^-L1 1-\LL Concentration Parameter CONTROL pH (S.U.) -4.32 DO (mg/L) 1,-, . T" L4f--. Conductivity 3 1 3tQ (iimhos/cm)37 Alkalinity (mg CaCO3IL) Hardness (mg CaCO3/L) CA-Temperature (C) vs.O LS"L. t .O ., . .". PH (S.U.) 7H4 17 0 ý 11.3% DO (mg/L) ~7-Conductivity . .. Temperature(°C) ( .'QO 7M-0 LS.O 7. .1 pH (S.U.) 3 ;4A-; II 1 4-,L I II T1- a I :li3 H 1.22 1 22.6% DO (mg/L)., T S, -- - L *I ". !Li-Conductivity (11hos/cm) ] 4- of,
, Iuil p~iatui* I[ t.* *,
b em era re I" 45.2% DO (mg/L) ' Conductivity (ýimhos/cm) 10A - R 1q JL4 Temperature (TC)
!PH(S.U.) t . I 3 i3 I. i -
72.6% ~jDO (mg/L) Ii~J- 17L7L Conductivity (Timhospctu) Temperatur (TC) I 1-Ls . '.,q9 - pH (S.U.) 3.33 I --4. 1 II II ,1-.zo I 100% DO (mg/L) q-*- -4 1-, I -z-* ... "4-? Conductivity (pimhos/cm) Alkalinity (mg CaCO3/L) Hardness (mg CaCO 3/L) TR chlorine (mg/L) I< 0.11) Temperature (QC)
* - 7'1.A I 1-1 1S. 1 .. i q. -"4.7 pH (S.U.)
p I~ I 3.3;)
-1 Z I1
.*o I13o I II '} ,1 I 100% Intake DO (mg/L) 1I Conductivity Alkalinity .r8 (mg CaCO 3IL) 1 S0.\
Hardness (mg CaCO3/L) (A TR chlorine (mg/L) . 0.10 Temperature (TC) 7-4.H .* i I e. * .. t a - a II
- II *
- Initial Final Initial Final Initial I Final "
Page 48 of 107
- Page 7 of 7
* .- mmha E elngS~lmIc Species: Ceriodaphniadubia Client: Sequoyah Nuclear Plant - Non-treated *Date: 05- %"L-cf-U I I Day I1 I
IS - 3 I 4 I 5 I 6 II Analyst I Concen- I Parameter tration CONTROL pH (S.U.) / DO (mg/L) ~1. P Conductivity I 32_1o 33F3 (Aimhos/cm) Alkalinity (mg CaCO/L) HardnessI Pei0 (Mg CaCO 3/L,) Temnerature (ofl "9 pH(S.U.) 1,2cp (t',p ".4 .
. 7 7.Ac . 1. b 11.3% DO (mg/L) M Conductivity 30q
_____ gLS. -LA 1. 1 .0 bV vb/ 0 Conductivity ( CQ ~4 IA.-AA~x~ ____Temperature pH,(S.U.) (_V )*,* . . .3* 141q
"*z 25.2% DO mg/ll, (pmhosicm)
Conductivity (4 PH (S.U.) Temperature(°C) iQL. o - .'- 1 I 7 ,4 * / I I 7 L *" 72.6% Conductivity 'DO*mg/fl_ (prnhos/cm) II7 p.¢U.) PH 4,1 -1 777,* *. Temperature (°C) 05.0 2s".';.. 7A.- I.qk .L " "* . 7200% DO (mg/L)9q mhIos/ m) *-2-X WI Conductivity PH_(.U.co) q.O 100% DO m.) Hardness *' (rag CaCO3./L) .00 TR Chlorine (mg/L) <0.10 Temperature (C) PH (s.U.) I5)I73 0 I. . 1U. VA 1l 100% DO(gL Pmhos/cm) *I']6I '+4 . Alkalinity "-2 l (mag CaCO3/L) S"Hardness TR chlorine (mg/L) < 0. 1c) Temperatur (T -. o -q.*e 11z. , zl I Initial 1 Final I Initial 11 Final I Initial 11 Final I Initial I Final ,1 Page 49 of 107
Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: TVA County: Hamilton Facility: Sequoyah Nuclear Plant Treatment: UV-treated NPDES #: TN 0026450 Outfall: 101 Project #: AALk Dilution preparationinformation: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Each concentration was treated Effluent volume (mL) 282.5 565 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2217.5 1935 1370 685 0 to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences. Test organism information: Test information: Organism age: 7o.Ss Aooc, 1j Randomizing template: S%*Je-. Date and times organisms o - \ o Incubator number and were born between: __shelf location: Organism source: týr &'iUA Pp M -I\-o1 Artemia CHM number: L,.LA44 Transfer bowl information: pH = SU Temperature = 'C Date / Time in: 05. A-ON %oo
" Date / Time out: OS Zo-Cn It.2O Average transfer volume: Total drying time: tq.-.U t$,vt "Ths
- u. Initial oven temperature: ko(" ^U Final oven temperature: oV U Dailyfeeding andrenewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Sample MHSW or termination numbers used batch used Time Analyst Time Analyst Time Analyst 0 o'Z,- -it-cft _____ 1-Do3 . VZ:5L.. WCfN o 4-LO oS-d9-OOA 1---_ _ _ _ _ _ _t) 140__ I__-____ c c -o , os.-t'-dc S 3__ c*s-la-ý,oc OS.d I'S. cr odocl 00 A, ,I Ldo A22 It~~ 2C)5 M* IZ*5"105.*
*O'1 o--
OS-DO'-11, 1 4 cfs OýPoo 4L Iqc Qc 2A a G~l-r,4 OI US- 12-cPR 6 0 lq3-1 Gs -is- 0 1o oA3,4:- 6qO51r, - O-Z 4-ni n<-11-cft 7 -f0- Oc _ _ _ _ _ _r_' figo Control information: Acceptance criteria Summary of test endpoints:
%Mortality: < 20% 7-day LC 50 > It 07.
Average weight per initial larvae: 0. 6 &- NOEC o00"7.. Average weight per surviving larvae: O. (0VL4 _ 0.25 mg/larvae LOEC I 14 ChV IC2s . >tool. Page 50 of 107
Page 2 of 6 Species: Pimephalespromelas Date: OC-v2--ol Client: TVA / Sequoyah Nuclear Plant - UV-treated Survival and Growth Data Day CONTROL 11.3% 22.6% A B C D E F G H I J K L 0 I 10 10 /t to //0 0 '0 /0 /0 10 /0 2 10 O to t0 It o0 /0 /o t / /0 // 3 C/1 0 to /0 /0 ip ./ C0 /0 t0
/o /0 50 Io 0t /0 (0 /0 !0 /0 /0 (0 '0 /0 /Q0 650t /0 1I0 DD t Dto I t to iI toOt' IO O I0
/c 10 ,/
C 6 I0 10 ( ) 0 / 1Q /D to (0, 10 ' /10 10, 10 10 t0 (0 'o (0t (0 j0 to to A = Pan weight (mg) Tray color olf::
- lt )3,*g 13-0,* 13.8o i~lyý )4.60 )34*7 ý-60 /oItt 13.-9, tK ILL 14 ILL 1 Analyst: C?*Lf-Date: p0j9 -- 0 _
B = Pan + Larvae weight (mg) P. . 3-1.o1q -. 35 , . Ib a.I.h'L,U JA-3 ;J,4* .l.'b ).0i, Analyst: \ Date: 0 5" -'&,0 - 00, C = Larvae weight (mg)
=B-A .- 1s.4s 0,,,6. (#.sot 3a
-p S, I 1-56 a1. 11 -rz .. b .. ' .* *.
Weight per initial number of larvae (mg) C, 'V SID & 4.
'a CA. I'.
= C / Initial number of larvae to, N A*
0* Average Percent A ~a f a a aba weight per reduction initial from control 0. IOR lranumber of (% Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: _4& Comments: Page 51 of 107
Page 3 of 6 FT
,*Env~wnm-1mTWfllqS~utlo* 1nm Species: Pimephales promelas Date: OS -1L--CYN Client: TVA / Sequoyah Nuclear Plant - UV-treated Survival and Growth Data Day 45.2% 72.6% 100%
M N 0 P Q R S T U V W X 0 0 M 10 /D 1 /0 a /I b /0 /t0 tO 0 1 /0 10 /D /0 /0 /1 /0 /0 /0 /0 /0 /0 2 / C0 IC ( /0/0 10 0 to /0 /0 /0 /0
.0 31 I0 1 ID 0 t0 10 to /0 I/0 10 4
I QO t 0_/0 ID 100 '0
/0 /0 10 / 0 /0
/0 /Q
.5 to 10 1o ( /0 /0 to /0 /0 0 10 /0 6 I (Q I.
10 (0 I 10 C IL) K I/lk '0 /0 10. 70 ID 1- I( 10 10 /0 /0 /0 IO io It) A Pan weight (mg) Tray color code:: IN.Iq" Analyst: r-L, 5-r1q I.oa 1o 13.q 31ý" 1-8 Jq.sT l~ t l 1-q ).'31. 0 ),toL.-
- t. J-S 34 I.. Ii" I?.jI 1.I Date: 6 ""I. *;
B = Pan + Larvae weight (rag) P-*S?ý [.I! IT1,qa, P.1-D)l p.s"o .l o l p)0,T*6 /q~ql ;.lot4 g 4o o.Vq Analyst: _r ALsr" Date: QT"-0 - 0 q_ C = Larvae weight (mg)
=B-A Weight per initial number of larvae (mg) pv '
=C / Initial number of larvae., " *I o" 0" 0" 3" *" " *" O O' Average Percent
.weight per reduction initial from control LAA 0.1., A- -'I *.170.V Z 2..7T .
number of (%) larvae (mgB) Comment codes: c clear, d dead, fg =fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Comments: Page 52 of 107
Page 4 of 6
- )ETS Species: Pimephalespromelas Date: O'S- 7 -Oq Client: TVA / Sequoyah Nuclear Plant - UV-treated Survival and Growth Data Day 100% Intake Y Z AA BB 0
2/0 /0 /0 /0 to me 4 . 5 to /0 /0 6 IQL 0 ( 10 to to to A = Pan weight (mg) Tray color code:: 1ý t.1 1 jl.Ri 1 ',D i1.,qL1 Analyst: r"Lr Date: D* * "t-O- O B = Pan + Larvae weight
.(rg) -. I ý.lql'U- g.a Iq3js Analyst: nL f",
Date: 99- 3.0- Ott C = Larvae weight (mg)
=B-A Weight per initial number of larvae (rmg)
= C /Initial number of larvae \,a 'a Average Percent weight per reduction initial from control number of (%)
larvae (mg) Comment codes: c = clear, d = dead, fg fungus, k = killed, m missing, sk sick, sm= unusually small, Ig= unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 53 of 107
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 12-19, 2009 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses '. Environmental Testing Solutions, Inc. Project number: 5444 Reveiwed by: /'-(i YiA[l--- Not for Compliance Assessment Internal Laboratory QC Cusaeetratioa (% Rqplicate
- aItala.mbeod Find uu.beraof A P -right B=an+La-,.'a L.ac oight(.g) WWigktJS-riviMg M. dghtI C/adfleta-riatio Wdgka Ilidit aber Meau-uvhu.l M-a eight It CoCeffliPaerl Pent rcmtnuc ioafrou tnrva -Lrvac (-9 wright (rg) -A-B .mbnruofirvae(maW S. rvguiA.mbrrof -1 , ofalarvua(mg) (%) lIlallunmberof vrarido (m.-O, coatrol(%)
- larvae(ug)
- urvae (erg) ~Me....
A 10 I0 13 85' 20.22 6.37 0.637 0.637 " Control B 10 10 13.62 21.05 7.43 0.743 0.684 6.8 0.743 100.0 0.644 6.8 Notappleable C 10 o0 13.80 20.75 6.95 0.695 0.695 D 10 10 14.45 21.04 6.59 0.659 0.659 E 10 10 14.60 22.35 7.75 0.775 0.775 F 10 10 13.77 22.29 8.52 0.852 0789 5.4 0.852 G 10 10 12.60 20.16 7.56 . 0.756 0 0.756 100.0 0.789 5.4 -15.4 H 10 0 14.1 4 21.86 7.72 0.772 0.772 1 10 10 13.86 21.23 7.37 0.737 0.737 22.6% . 10 10 14 15 21.42 7.27 0,727 0.727 K 10 10 14.14 21.20 7.06 . 0.706 0.724 1.8 0.706 975 0.706 5.3 3.3 L 10 9 14.31 20.84 6.53 0.726 0.653 M 10 10 15.59 .2252 6.93 0.693 0.693 45.2% N 010 o 13.95 21.17 7.22 0,722 0.114 4.6 0.722 100.0 0.714 4.6 -4.4 0 10 10 13.09 19.92 6.83 0.683 0.683. P to 10 13.45 21.01 7.56 0.756 0.756 10 10 13.68 20.50 6.82 0.682 0.682 72.6% R 10 10 14.35 21.03
- 6.68 0.668 0.684 3.8 0.668. 100.0 0.684 3.8 -0.1 S 10 10 14.41 21.06 6.65 0.665 0.665 1 T 10 10 13.34 20.56 7.22 0.722 0.722 U
u 10 10 13.60 19 91 6.31 0.631 0.631 V IV 00 1440 21.04 6.64 0.664 0698 8.5 0.664 W 10 10 14.19 21-.70 7.51 0.751 0.751 X [0 10 13,44 20.89 7.45 0.745 1 0.745 Y 10 10 - 14.20 21.15 6.95 0.695 0.695 100% Intake Z 0 10 1482 21 17 6.35 0.635 0.659 4.1 0.635 AA i0 1o 14,20 20.82 6.62 0.662 0.662 100.0 0.659 4.1 37 1B 10 10 12.96 19,38 6.42 0.642 0.642 Outfall 101: MSD = Minimum Significant Difference. Durnett's MSD value: 0.0720 PMSD = Percent Minimum Significant Difference PMSD: 10.5 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Intake: Dunnelt's MSD value: 0.0520 Lower PMSD bound determined by. USEPA (lOth percentile) = 12%. PMSD: 7.6 Upper PMSD bound determined by USEPA (90th percentile) 30%. 200 Lower and upper PMSD bounds were determined from the loth and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 1a; USEPA, 2001b) USEPA. 2001 a, 2001b. Final Report: interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B.-01--04 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
TVA / Sequoyah Nuclear Plant, Outfall 101 (ti U V-treated "-a May 12-19, 2009 T Statistical Analyses
,* Environmental TestIng Solutions, Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample lD: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% Y- 2 3 4 D-Control 0.6370 0.7430 0.6950 0.6590 11.3 0.7750 0.8520 0.7560 0-7720 22.6 0.7370 0.7270 0.7060 0.6530 45.2 0.6930 0,7220 0.6830 0.7560 72.6 0.6820 06680 0.6650 .0.7220 100 0.6310 0,6640 0.7510 0.7450 Intake 0.6950 0.6350 0.6620 0.6420 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6835 1.0000 0.6835 0.6370 0.7430 6.776 4 0.7361 1.0000 11.3 0.7888 1.1540 0.7888 0.7560 0.8520 5.450 4 -3.522 2.410 0.0720 0.7361 1.0000 22.6 0.7058 1.0326 0.7058 0.6530 0.7370 5.308 4 -0.745 2.410 0.0720 0.7096 0.9640 45.2 0.7135 1.0439 0.7135 0.6830 0.7560 4.598 4 -1.004 2.410 0.0720 0.7096 0.9640 72.6 0.6843 1.0011 0.6843 0.6650 0.7220 3.834 4 -0.025 2.410 0.0720 0.6910 0.9387 100 0.6978 1.0208 0.6978 0.6310 0.7510 8.544 4 -0.477 2.410 0.0720 0.6910 0.9387 Intake 0.6585 0.9634 0.6585 0.6350 0.6950 4.083 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.957272053 0.884 0.185406404 -0.99302071 Bartlett's Test indicates equal variances (p 0.83) 2.111745119 15.08627224 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.072014927 0.105361999 0.0061735 0.001785833 0.023020582 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 95% CL(Exp) Skew IC05 60.362 ICI0 >100 IC15 >100 1C20 >100 IC25 >100 IC40 >100 IC50 >100 sqn101_05-12-O9data-uv
TVA Sequoyah Nuclear Plant, Outfall 101 - Intake -.Z U14 UV-treated May 12-19, 2009 0 0 Statistical Analyses
. Environmental Testing Solutions, Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% 1 2 3 4 D-Control 0.6370 0.7430 0.6950 0.6590 11.3 0.7750 0.8520 0.7560 0.7720 22.6 0.7370 0.7270 0.7060 0.6530 45.2 0.6930 0.7220 0.6830 0.7560 72.6 0.6820 0.6680 0.6650 0.7220 100 0.6310 0.6640 0.7510 0.7450 Intake 0.6950 0.6350 0.6620 0.6420 Transform: Untransformed 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.6835 1.0000 0.6835 0.6370 0.7430 6.776 4 11.3 0.7888 1.1540 0.7888 0.7560 0.8520 5.450 4 22.6 0.7058 1.0326 0.7058 0.6530 0.7370 5.308 4 45.2 0.7135 1.0439 0.7135 0.6830 0.7560 4.598 4 72.6 0.6843 1.0011 0.6843 0.6650 0.7220 3.834 4 100 0.6978 1.0208 0.6978 0.6310 0.7510 8.544 4 Intake 0.6585 0.9634 0.6585 0.6350 0.6950 4.083 4 0.934 1.943 0.0520 Auxiliary Tests . Statistic Critical Skew Kurt 0 0 Shapiro-Wilk's Test indicates normal distribution (p > . 1) 0.95557791 0.749 0.566287708 -0.42008898 F-Test indicates equal variances (p = 0.40) 2.96680498 47.46722794 Hypothesis Test (I-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic I Test indicates no significant differences 0.05203226 0.0761262 0.00125 0.001434 0.386520296 1,6 Treatments vs D-Control sqnl O_015-12-09data-uv
TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated -- May 12-19, 2009 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number: 5444 Environmental Testing Solutions, Inc. Reviewedby Concentration Parameter Da 0 Da I Da Da 3 Da 4 1 D 5 Day 6 Initial Final Initial IFinal Initial Fni Initial Final Initial IFinal IInitial Fnl Initial Fia pH (SU) 7.51 7.26 7.42 7.2 7.15 7.28 7.38 7.49 7.44 7.53 7.42 7.50 7.04 DO (mg/L) 7.8 7.7 7.6 7.5 7.5 7.8 7.6 7.81 7.61 7 7 Conductivity (limhos/cm) 313 296 317 3 Control Alkalinity (mg/L CaCO 3) 61 59 Hardness (mg/L CaCO 3) 88 92 Temperature (OC) 24.8 24.8 24.8.824.8 24.9 24.6 24.8 24.6 24.8 24.6 24.7 24.5 24.9 24.41 pH (SU) 7.52 7.20 7.43 7.24 7.40 7.15 7.29 7.34 7.49 7.38 7.53 7.38 7.52 7.081
. DO (mg/L) 7.9 7.2 .7.7 7.3 7.5 7.5 7.8 7.3 7.7 .7.3 7.7 7.5 7.7 6.8 Conductivity (plmhos/cm) 301 29 282 302 303 321
...... _Temperature ('C) 24.8 24.7 25.0 24.8 24.7 24.4 24.8 24.6 24.84 24.6 24.7 24.5 24.9 24.4 pH (SU) 7.52 7.19 7.43 7.24 7.39 7.11 7.29 7.28 7.48 7.394 7.54 7.371 7.50 7.02 DO (mL)
% 7,9 7.3 7.8 7.2 7.5 7.5 7.9 7.2 7.7 7.4 7.7 7.4 .7 6.8 22.6% Conductivity (pmhos/cm) 284 280J
- 28. 264 281 28 283 299 Temperature ('C)
_ 24.8 24.7 24.9 24.5 24.7 24.4 24.7 24.5 24.8 24.4 24.7 24.6 24.9 24.4 PH (SU) 7.51 7.15 7.42 7.18 7.34 7.11 7.28 7.30 7.44 7.38 7.50 7.32 7.48 7.03 DO(mg/L 7.9 7.2 7.8, 7.1 7.6 7.4 8.0 7.4 7.7 7.4 7.8 7.4 7.7 6.8 4.% Conductivity (psmbos/cm) . 247 244 ý 231 245 246 ý N 248 ý 258 Temperature (°C) "24.8 24.9 24.9 24.4 24.7 24.3 24.7 24.5 24.9 24.3 24.8 24.6 24.9 24.5 pH (SU) 7.47 7.19 7.38 7.13 7.29 7.12 7.23 7.27 7.39 7.30 7.49 7.36 7.43 7.03 DO(mg/L) 8.0 7.1 7.8 7.1 7.6 7.4 8.0 7.3 7.7 7.4 7.8 7.5 7.6 6.8
- 72. 6% Conductivity (ýunhos/cm), 204 200 189 206 198 197 2 Temperature (TC) 24.8 24.81 24.94 24.6 24.7 24.5 24.7 24.3 24.9 24.7 24.9 246 24. 245N pH (SU) 7431 7.191 7.34 7.14 7.25 7.11 7.19 7.301 7.34 7.29 741 7.28 7381.95 DO (mgL) 7.91 7.11 7.81 7.1, 7.8 7.4 8.01 7.41 7.7, 7.3 7.8 751 7.61 6 .8 Conductivi mhos/cm 159115 148 157 157 100% Alkalinity. (mg/L CaCO 3) 52 444 Hardness (mg/I CaCO 3) -62 64 Temperature ('C) 24.81 24.81 24.9 24.6 24.8 24.5 24.71 24.7 25.01 24.31 24.9 24.4 24.8 24.6 pH (SU) 7.43 7.111 7.31 7.13 7.19 7.01 7.15 7.2 7.311 7.29 7.38 7.24 7.361 6.89 DO (m 7.9 7.2 7.8 7.1 7.8 7.4 7.9 7. 7.8 7.3 7.71 7.5 7.6 68 Conductivity(Imhos/cm). 154 141146 100% Intake Alkalinity (mg/L CaCO 3) 57 5250 Hardness (mg/L CaCO 3) 66 62 8 Temperature ('C) 24.8 25.0 24.8 24.7 25.0 24.6 24.75 1.5 24.51 4 24.4 24.91 24.4
Page 5 of 6
*DT Species: Pbmephalespromelas Date: Os-1"_-20' Client: TVA / Sequoyah Nuclear Plant - UV-treated Daily Chemistry:
Day Concentration Parameter CONTROL pH (S.U.) DO (mg/L) ."r ' * : - J.* Conductivity " 13 (ý+/-mO*M) ýi Alkalinity (mg CaCO 3 /L) Hardness (mg CaCO 3/L). __________ Temperature ( . -
"Q. "AAk ".. L .9 '" A.,
pH (S.U.) .44 14 11.3% DO (mgb 1-)7 -4S Conductivity 7ý& (pnhos/cm) Temperature (0 Q 1A.k - LtS.-0 V. PH (S.U.) --7 1-4-43 T3q
-1,~ }
22.6% -DO (mg ). °b .,.9 1-5 Conductivity _________ Temperature QC) .4~iA AS t DR (S.U.) r % .... ] I "-;- II "7-s I T-,q3 U J- I 1 ".1-3 IUF.11 !I 45.2% DO (mg/b) 7 ,9 1 -'1.* 1 4-1 I ."1-4,q. Conductivity ý3L11(I____?_Hqa_3__ (Ltmhos/cm)
*l - n i
Temlerature (C) d.,q I 31,I
-- ? I Tq pH (S.U.) ,L 4 1-1 --* q ' 3 9.]1I f-13 I 72.6% DO (mg/L) Ell r. I .d I l
Conductivity Temperature ( iC) -IA-F< I 2A.9 lAlc~ pH (S.U.)
&~
-4.L/-?1 1 .3-4 II L4 II i-ý
- 1
ý -a 5 I II
.1 I1 100% DO (mg/L)
Conductivity (gmhos/cm) _____ Alkalinity (mg CaCOt3L) . "_.. Hardness (mg CaCO3/1__ TR chlorine (mg/L) Temperature (°C) 2 II 1 PH (S.U.) "? 3 1 100% Intake DO (mg/b)
* " Conductivity
..*myrhos/cm) }**]*2I/
Alkalinity (mg CaCO /L)62 Hardness (mg CaCO3/L) 67-TR chlorine (mg/L) < 0. '0-0 _ Temperature (°C) 2q-*"S.D
? 94 j - I ". - C8 ,
Initial 11 Final I. Initial I Final I Initial I Final Page 58 of 107
.Page bT 6 of 6 Species: Pimephalespromelas Date:7 u - 112.-01)
Client: TVA / Sequoyah Nuclear Plant - UV-treated op 0\e' U Ip Day, N I I 0-3 I 4 I \5 I 6 I Analyst Concen- Parameter tration CONTROL DH (S.U.) DO (mg/L) - 41 1- TO Conductivity (u.mhos/cm) Alkalinity (rMg CaCO 3/L) Hardness (mr CaCO/L) S Temperature (MC)
" " " I
.* "-.A. -. iA. n .S Sol. - -. .
pH (S.U.) I 11.3% DO (mg/L) -1.3! Conductivity (iimhoslcm) &2t2. 1A m~ 1-1.7T 303 Temperature (MC) ~2A 1 pH (S.U.) 22.6% DO (mg/L) 4 Conductivity (ýimhos/cm) 2E ý3 ______Temperature ('C) 2'-. f~S1'~ 1+-1 1. pH-(S.U.) 0 1..49 * -34.7 , 45.2% DO_(mg8L0 7 .- -7 . Conductivity - (p~mhos/cm) Temperature( 0 C) 1 -A.7 '--" "., 7. pH (S.U.) __7___3 72.6% DO (mg/L) . 7-1 Conductivity (gmhos/cm) ______Temnperature ('Q) Iq~ "t~1L~~-A1 A74~ 4 100%. DO (mg/L) Alkalinity m (mg CaCOJL1)
- Hardness (mag CaCO1/L) WD0 TR Chlorine (mg/L) < 6, ID
__ __ __ Tern erature ('C).1 *s m+ .... . - - [ -. a " pH (S.U.) I Q 16 *.v7
-7 I 7.371 II ', I -*1.2 1' m2"L.' I -4. 1 AI ,q I 100% DO (mg/L) I -I-'.(4 Intake Conductivity pmhos/cm)
Alkalinity SO (mg CaC h/L) Hardness TR chlorine (mg/L) 1;o. ,0 Temperature (QC) a ""'l I ý-' I is."0 I 'j 1I 1'7 1 7.4'-- I -'. l I Initial Final I Initial Final I Initial H Final I Initial 11 Final I Page 59 of 107
Page ot,1 Page I of I ng ETS Environmental TestingSouinm
-56's%
Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water,'RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst F7% L7 77 Iodide reagent: 1AR 334 Date analyzed 5 - )a. 6 ] Acid reagent: In 3-3ý d Calibration: 0.10 mIL 1.00m I Reference standard number I XA~s ý13 Ai 7 I Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratorycontrol standard: Reference standard True value (TV) Measured value (MV) % RS = MV /TV x 100 number (mg/L) (mg/L) (acceptable range =90 to 110%) I 3%s 67-3 0.50 I. Duplicatesample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD = ((S - D) /[(S+D)/2]} x 100 number (mg/L) (acceptable range = : 10%) Duplicate D o,0a 1. ----- -1"- Sample measurements: Sample Sample ED Sample characteristics Residual chlorine number B (shoud be < mg/) 0dd0113 tI 00-L ba 1\ J f\ t"16' 0 ,' Di'o)4 L-o~b Note. All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratorycontrolstandard: [ Reference standard True value (TV) Measured value (MV) % RS - MV / TV x 100 number (mg/L) (mgIL) (acceptable range = 90 to 110%) I 1/15 3+ I-044flI I Reviewed by I ::
- Date revieweda lage 60 of 107
Page -5 1 Page_ "-of i C D E nvironmentallTesting Solutions, In. Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Meter: Accurnet Model AR25 pH/Ion Meter Analyst F t" I77]r Iodide reagent: F I_/23 1 I Date analyzed LI5-II -E9i Acid reagent: It% A 3 Calibration: 0.10 mg[L I 1.00 mg/L I [Reference standard number I XA(,S M 1 Tl.-S Mote: For samples with a residual chlorine of > 1.0 mgiL, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratorv controlstandard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)
-Z) 3 0.50 0, tif q4. e Duplicatesample precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD= {(S -D) /[(S+D)/2I) x 100 number (mg/L) (acceptable range = .10%)
- Duplicate r'.
Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number mgIL Blank (should be <0.10 mg/L) '-ood7 t Oct ut%4. 01 ,1ýrk 6a " 33 ?'Y6 1 &Ij4, ~Oo qL, 31 1o51'5.¶ t S-0 ' i ?) IXe,C,16 Ab-,14 d-io , -331 0-i 17f'+J,
-"%&ý Ab, Mote. All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Reviewed by. [- Date reviewed age 61 of 107
*Lict 0Page3L2 C 4 C~t age I of _L Environmental Testing Solutions, Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mgiL Meter: Accumet Model AR25 pH/Ion Meter-Analyst L 7 7 Iodide reagent: --- 711337 Date analyzed oc-IL9_ OC* Acid reagent: [cr_ 2DE Calibration: 0.1 mg[L oo 1.oo mg*L Reference standard number T NSSU-7.3 Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratorvcontrol standard: Reference standard True-value (TV) Measured value (MV) % RS=MV/TVx 100 number (mg/L) (mg/L) (acceptable'range= 90 to 110%)
"ThSbto'13 0.50 0.501 Duplicatesample precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD ((S - D) /[(S+D)/21) x 100 number (mg/.) (acceptable range- +/- 10%) _.615, 0 S e-o.oco40-4 .Duplicate D LO.000113 -. Sample measurements: Sample Sample lID Sample characteristics Residual chlorine number.- (mg) Blank (should be = <0.7-mg7 40-.O.012-.9 M 1U .o 41 tai ih CLci ctMfo , Cn l0orY o.-0344 O96qj-Di* .-Scr O~*. SrcoId-kJ. t RC)4 1-fi ttfs s\.9V+/-1djtou VIktqto tn pa~~ot~fbY,.
- L-"C'.DlSt LO.ootsI, Q~~~~~~
d~ S0iIA0AN ~ ~ -k OG I, loidai (.,
- PCH, , ,.W 0Lio4 o,. 3 'P4\*'4TP r*Jt- d N C')C-0 0,-C0o.o0X gL 0, 00711 IOct ýO I-~ SA
____V rl-O dooj-e- +&o~ ~.O. Note: All samples were analyzed in excess of EPArecommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (rg/L) (Mg/L) (acceptable range= 90 to 110%) T__S.ci- "f t
- 0.50 OL . 6 q 2.0 V/.
Reviewed by Iage 62 of 107 Date reviewed LtO'-2rr -43
Page 32.
"ErS
" EnvivnmentalTesting Solutions, Inc.
Page 3- of *.c Alkalinity (SN2320 B) Matrix: WtCe, RIL = 1.0 mg CaCO3/L Analyst I tIII7-7 Time initiated "
,Date analyzed 0 - - j] Titrate samples to Time completed EIitIZZ1 pH = 4.50 S.U.
Titrantnorm ality and multiplier determination pH of Normality Normality (N) of H2S0 4 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na 2CO 3 x 0.05)/E = (Nx 50000)/ 100 ml sample water reference standard ml ml ml 00.25/E = Nx 500
=4.5 S.U. number number (E) (acceptable range = 0.0180 - 0.0220)
- a -. - .- 10 Laboratorycontrol standard:
Reference standard True value Sample Alkalinity (MV) % RS = MV! TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3 IL) (acceptable range (mg CaCO3/L) (ml) ml ml ml =90 to 1.10%) ITS 'S 454 100 100 11.'4 a 'o 9*. 10.1 -'LOq Duplicatesam le precision: Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO3VL) ((S - D) /[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range
- 10%)
06-o t4-o)M M1R", k4
- too OXc co *.3. C5S sn U.-I g a Duplicate (B) D Matrix spike recovery:
Referencestandard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (lig CaCO3IL) (mg CaCO 3/L) (m[) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO3/L) MV = A - B (acceptable range (mg CaCO3/L) = 75 to 125%) Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOgL) o ,o -bcit) f S 0O 7,10L Q).-+ b!_ - ,3Uo6S-0 -~ 2 A___- ____ I 05.oS- 0 oq'q tn_ -. , ,, _ 2 b~LY~__ I~~35_~0 6 1 I 0i W_______c~ ',4. 4. )CIO_ __ age 63 of 107 Reviewed by: Date reviewed: Darvw:s. I t05A
Page 83 Page c-2 ofL Envi..nmtnt
-,* Tolg Solutions. Inc.
Alkalinity (SK,2320 B) Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst I~s] Time initiated Date analyzed mliier Titrate samples to Time completed pH - 4.50 S.U. Titrant normality and m ultiplier determinationi p of1 D Normality (N) of H2 S0 4 pH Factor or Multiplier Deionized Titrant check egin I = (5 ml Na2CO 3 x 0.05)/E = (Nx 50000)/ 100 ml sample water . reference standard ml ml ml Nx 500
= 4.5 S.U. number number (E) (acceptable range - 0.0180 - 0. 7t Laboratory controlstandard:
Reference standard True value Sample Alkalinity (MV) % RS MV / TV X 100 number (TV) volume Begin End Total Multiplier (rag CaCO3IL) (acceptable range (mg CaCO3 /L) (mi) ml ml ml =90 to 110%) i ,oss f 4
'Duplicatesample precision:
100 100 Sample 340 43.4 4
. 1-,.f Alkalinity %RPD =
S'1 Sample number -_(ml) Sample ID volume Begin ml End ml Total ml Multiplier (mg CaCO3/L) (S- D) /[(S+D)!21) x.100 (acceptable range
- 10%)
4 Duplicate,(B) s .-- D 1..t w Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml). ml ml ml Multiplier (mg CaCOAL) D-cnocO 144 T *o~i-1V 10 bQe ______ 4 -3 (
- a-: N~A A-, .OL
-ý , G- __________ - Iý___
rA t . 6&MIIIN-, L)). I ý' b091 __ _n
'14 gh AS n-1 i (ý - 1 I
-~ ~~~~~~~ ______________ _ _ --
i-I_______ t,__ I i~ ------
^C4, nS ,Aq. 04 S_-5
* .77
( 2P~ Y 4 t II yi..J.~.'.. ýX-r, .'Al I oil r9 0'- - PH IOA,90 L '-~'~-*'~ ..-ftl
*-' - J I tpD e 64 of 107 Reviewed by: : Date reviewed: OOQ
Page _____ Page 3of-6
- rEmnmontaJ TIN5l1. 1- In"c.
Alkalinity (SM 2320 B) lvlatrix: Water, RL = 1.0 mg CaCO /L 3 Analyst[ 7 t Time initiated Date analyzed - Titrate samples to Time completed pH = 4.50 S.U. Titrantnormality and multipier determination: pH of N - Normality (IV)of HZS0 4 pH Factor or Multiplier Deionized Titrant check Begin a9--_._ 5 ml Na2CO3 x 0.05)/E (N x 50000)/ 100 ml sample water reference standard ml ml ml 0 N-x 500 4.5 S.U. number number (E) '(acceptable range= 0.0180- 0.0220) Laboratory controlstandard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3 /L) (acceptable range (mg CaCO3/L) (ml) nml ml ml = 90 to 110%) T S, 64 100 100 t)u, . q-, .'- I94 10I C, Duplicatesa le precision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3fL) ((S - D) /[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range=- 10%) I Duplicate (B) 4, D, ___,.~f~ '__- -:Tto trPto Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3IL) (mag CaCO 3/L) (ml)
- ml ml Sample alkalinity (B) Measured spike value (MV) % R MV SV x 100 (mg CaCO 31L) MVCaCO31L)
=A - B (acceptable range (mg = 75 to 125%)
Sample measurements: Sample volume
- Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mag CaCO3IL) o0kos0 ,ol ( q ,._÷ 07.:S -D + L_,_,o_,_ _
io014,.2*.9*A
, - -o- - .. , .
Reviewed by: z~IIj Reviwedby:Date reviewed.: 1C5 -
Page
- Em"T jRlons. Nov SEnvinmnmentalTes'n Solut Iam.,Inc.
Page 4o.if' Alkalinity (_SMi320 B) Matrix: Waier7RL = 1.0 mg CaCO3/L Analyst pS-Time initiated [ CI77] Date analyzed 05Z 0-bi Titrate samples to Time completed pH = 4.50 S.U. Titrant normality and multiplier determination: of Normality Normality (AI)of H2 SO 4 pH Factor or Multiplier Deionized c-----cht,-L. Begin End Total =(5 ml Na 2CO 3 x 0.05)/E = (Nx 50000)/100 ml sample water reference standard ml- = 0.25/E = N x 500
= 4.5 S.U. number number ___ (E) -______
acce180- 0.0220) Laboratorycontrol standard: Reference standard True value Sample Alkalinity (MV) % RS MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml =90 to 110%) [ Zs% - 10 0 100 . . .:' Duplicate sam lie precision: Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO3 /L) ((S - D)/[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range = :h0%) Duplicate (B) 2 oL D Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L) (Mg CaCO3/L) (ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R = MV ISV x 100 (mg CaCO/L) MV = A - B (acceptable range (Mg CaCO 3/L) = 75 to 125%) Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml mi ml Multiplier (mg CaCO3 /L) o'~a .og -pCAI3Lr= $3 3~z.* *3 L*I \b' t o 00, Os-O*'Q 0 --. 3. 5.3 .1.J).4i I .10ll\ 65 0 02R D~S 0__ __ 1 A40.V5~ 1CO ~III o0t0,;o *S0"O' STVA-weri j I all 1g .50to -16'1,1 g I I : ,. '1/291 '3sL*
- CA oSoa. I( 4 J I- 4 4 I + 4- 4~ +/-
o0 oCLs oq.O 1/- tA ) in LrI* 4~L~
. A e,*CJL, -XI -1
- J - ______________ ~---.~' I lage 66 of 07 Reviewed by: [II EII] Date reviewed: [ ? -ihoý t
'PPage . O Page_ _56 l.(-of Alkalinity (SM 232o B)
Matrix: Wate-rkL = 1.0 mg CaCO 3/L Analyst . Time initiated [ I Date analyzed L,ý Titrate samples to Time completed pH = 4.50 S.U. Titrant normality and multinlier determination: p Normality Normality (N) of H 2S0 4 pH Factor or Multiplier Deionized Titrant -- Begi _nd Total (5 ml Na 2CO3 x 0.05)/E = (Nx 50000)!100 ml sample water reference standard ml ml m- 25/E =Nx 500
=4.5 S.U. number number (E) (acceptable range= 0.018 Laboratory control standard:
Reference standard True value Sample Alkalinity (MV) % RS = MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (mg CaCO 3IL) (ml) ml ml ml =90 to 110%) I ss* 4-100 100 (g ,'.h, cl_ .4L Duplicatesam le precision: Sample Alkalinity, %RPD = Sample Sample ID volume Begin End Total Multiplier (Ing CaCO 3/L) [(S - D) /[(S+D)t21l x 100 number (ml) ml ml ml (acceptable range = +/- 10%) Duplicate (B) OD U ,. Matrix spike recovery: Reference standard Spike value Sample 'Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (rag CaCO3 /L) (mg CaCO 3/L) (ml) ml ml ml - Sample alkalinity (B) Measured spike value (MV) %R=MV/ SV x 100 (mg CaCO 3/L) MV = A-B (acceptable range (mg CaCO31L) 75 to 125%) Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (Ing CaCO 3/L)
*oSo*4.0S "T-VAIW6n 60 3o.r a,!5 1 .I-) LI,"4 q9
*015 2 ___4 (-- iL a 1U&03 2,9'S 43_.3__12 0',DcO, OSO,!..Oo.t ,/' B lb*.i2*l 2-.1 *.
44,-5.. -1 .- Iz 1 ,CP
-y 15Dt .
bot '1A4.cQ -1
. .... Wfb Ou ,, -- m-I ~
t 0 . I I * -. ~ - ~ I. I 4 I 4 0 .9 011 ~Lj. 4-13 I age 67 of I I 07 U -j - ... Reviewed by: " I IZD D ate reviewed: w ( -l Oe
Page ___ Page. of Lo, Alkalinity (SMW320 B) 4atrix: Waefr-td, = 1.0mg CaCO 3/L Analyst [757- ] Time initiated [ [ Date analyzed 10-bq Titrate samples to Time completed -" I ' pH = 4.50 S.U. Titrantnormality and multiplier determination:
-t-Normalit Normality (N) of 142S0 4 pH Factor or Multiplier Deionized Titrant check Begin - - - I Na 3 X 0.05)/E (Nx 50000)! 100 ml sample wer reference standard ml ml ml 5 -N x500
= 4.5 S.U. number number (E) (acceptable range = 0.0180 - 0.0220)
Laborator controlstandard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3IL) (acceptable range (mag CaCO3IL) ml) ml ml ml =90 to 110%) Duplicate sam le precision: . Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO31L) ((S - D) /[(S+D)/2I) x 100 number (ml) ml ml ml (acceptable range =
- 10%)
Duplicate (B) b*0 D - Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L) (mg CaCO3fL) (ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 1.00 (rag CaCO3/L) MV = A - B (acceptable range (mng CaCO 3/L) =75 to 125%) Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3IL) oosot."D 1-21670J 2 62 I 0 l b-i SU. O's o'-'O*q( - bi2-i - 504- Q.5~Wi_____ o'ic~1.s n- W- ~ 9 ' 2sp0 114 0 39y,} qq~ o'ip~~cfl.O10 0 jj j %P 00
~js-o1.o¶A nAA V r%,, 10O Q-ý 3 159 I ______ I t 1.
- I I I - 1.9sbl _
l'age 68 of 107 Reviewed by: IZýý Z Date reviewed: F7 0 Z
Page ____ 0
~ * -
Page 1 of- 3 1.* *'- ** Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst f* 7 Time initiated f 7&. Date analyzed Lii. J Titrate samples to Time completed I*' pH = 4.50 S.U. T,~fr,,n' normality and multinlier determination: a-t . . no ... .... .. .. . . . de . . . .. . . . _ __ pH of Normality Normality (A) of H 2S0 4 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na 2 CO3 x 0.05)/E (N x 50000)!100 mr!sample water reference standard ml ml ml = 0.25/E = N x 500
= 4.5 S.U. number number (E) (acceptable range = 0.0 180 - 0.0220) 6.0 INmF3,9 T-1,.S kp,53 t 1. ,L, IIP _ _ 1U..09.rT
_.c_ Laboratorycontrolstandard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (Mg CaCO3 /L) (acceptable range (mg CaCO3IL) (ml) ml mi ml = 90 to 110%) 94100 i~ 1L RO - Duplicatesam le precision: Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) ((S - D)/I(S+D)/21) x 100 number
** fU o (ml) ml F**~*ml
** ml I4 s tc (acceptable range = + 10%)
as - 2Xjo' MKS WIG2 100 C,.3 9~5 .5 10J01 S l-0C' 4 Duplicate (B) __ .- D Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number [(mg (SV) CaCO 3IL) volume (ml) Begin ml End ml Total ml Multiplier (rag CaCO 3IL) Sample alkalinity (B) Measured spike value (MV) % R= MV / SV x 100 (mg CaCO3fL) MV = A - B (acceptable range (mg CaCO3 L) = 75 to 125%) (c0 4:"".,. 50 l*ooCTD Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (rmg CaCOV/L) 0643.Q5 IC .q _ _ _
-I"O 4-4 ija-5( 5~U_ _ __
__ __ __ __ __ __ ____ ___~5~ 1. ~4 _ __ 0S- %'z-GP- 4 I1.0 q* 1 0q
-+~~ ~~~~ _ __ --- ~--- L4-~
06-1 zor I q-C-' rr--,A . . . . II . J'. 44 q.,,* k.... _ .
.H-Q-
+ I*=~-~------+ ~4 4 ~ 4 -
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~
i
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. n,.
A .- 14i"I
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2.< -2-fC- k
.I :)4- 3-1+----+ , 1 Q ~ ~ i~ L,+/-1.-~- -39 Reviewed by:
lage 69 of I 07 Date reviewed: [ -[:
Page 39 Page c of. 3
~,'Enironmn~,tA T"tIg Soluions, Inc.
Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst
- Time initiated Date analyzed 0 q.o"- Titrate samples to Time completed pH' = 4.50 S.U.
Titrantnormality and multiplierdetermination: pHo6T Normality (N) of H2 S0 4 pH Factor or Multiplier Deionized Titrant check e Total = (5 ml Na 2 CO 3 x 0.05)/E = (N x 50000)/ 100 ml sample water reference standard ml ml ml-- - = .5/E = Nx 500 4.5 S.U. number number (E) (acceptable range = 0. - ý Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV/TV x 100 1 number (TV) volume Begin End Total Multiplier (mag CaCOgL) (acceptable range (Mg CaCO 3/L) (mI) ml ml ml =90 to 110%)
- 100 100 b.o0 q.4 Co_42o0p
- it).q Duplicatesa ple precision:
Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCOyL) {(S - D) /[(S+D)/21} x 100 number (m_) ml ml ml (acceptable range = +/-10%) Duplicate (B) D Matrix spike recovery: _ Reference standard Spike value Sample 'Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3 /L) (rg CaCO3/1L) (ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R =MV/SV x 100 (Mg CaCO 3/L) MV = A - B (acceptable range (mg CaCO 3/L) = 75 to 125%) Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (Mg CaCO 31L) 0or OS106 l a ~ 3c' 1iSIC' -ý ~.1 17.? 4-0 44____ 0i1Lb'n 1 I -A-Stwkk .ý 60t . 1: IL - 3L. -I O QXlasbhO i - I 2
,h I ,QA,. (-i
*~~ ~ ~ ~~Z~ ~I 2~J 44 --
Oc b Ic~a .0-L. 1 _5 I g*, I
-+
WA-56aciT
.. 4 Afý C' 4--F~'-~--=-~ q 51-t ewjOsOSii. Q0 i S 7 7
I ----- I -44------ F'4 I ~1-fl-'..--F~-~-'d--4s- F I
-~
-Z I , k' a ^ ýI I 4I I 4-I I "- I I* 'II ' 41i
- -I-
-- r.f . I it4 i -, A 1 I -)1: - -
. C'a, . 08 2 'I b
.JC )+L4 A 2-*
of 107 Reviewed by: Date reviewed: -i f4 -0
9 1-"* Page Page 3 of 3 Ennkonment.I Tescin~ SnI,.tiws. Inc. Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst 7 c [ Time initiated Date analyzed ,,-I+ -
.IG Titrate samples to Time completed pH = 4.50 S.U.
Duplicatesam ple precision: Sample Alkalinity %RPD = Sample Sample ID, volume Begin' End Total Multiplier (Ing CaCO 3/L) ((S - D) /[(S+D)/21) x 100 number IVA (ml) ml ml ml I . (acceptable range = : 10%) Duplicate (B) .24o D Matrix spike recovery: Reference standard *Spikevalue Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier '(Ing CaCO3 /L) (mg CaCO3/L) (mi) ml ml ml 3;¢*t106* -50 11. ,. i.q150 ).-q. Sample alkalinity (B) Measured spike value (MV) % R = MV / SV X 100 (mg CaCOjfL) MV= A-B (acceptable range (mg CaCO3IL) 75 to 125%) L) allg 04 e 71 of 107 Reviewed by: Date reviewed: -iiLOZ
Page ___9 E Enviromt*ntal Testnqg lutis. Inc. Page Il of 6 Total Hardness (SM 2340 C) RL= 1.0gg CaCO03 L Analyst fZ_ 77E Time initiated t:q 0 Date analyzed o- - Time completed Titrantnormality and multiplier determination: Titrant reference Normality check standard I Begin ml End ml (acceptable range = 0.0180 - 0.0220)
=
pH Factor or Multiplier (I x 50000)/50 ml sample
=Nx 1000 II T) ~nI.,f to" a, 10p nrO',1*CM"*
Sample Hardness %RPD = Sample Sample ED volume Begin End Total Multiplier (mg CaCO3 /L) ((S - D) /[(S+D)/2]} x 100 number (ml) ml ml ml C)5.d-Cn&~ fydis 0O 7X) 50 D I(o. L .4-k' 2 S q Duplicate (B) - - - D Matrix spike recovery: __ Reference standard Spike value Sample Multiplier Spike (mghardness CaCOj/L) (A) (SV) volume Begin End Total number (Mg CaCO 3/L) "(M) ml ml ml Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (amgCaCO3/L) MV = A - B (acceptable range (mg CaCO3 L) 75 to 125%) Sampe measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (frgCaCO 3/L) TV = N Blank (should be = 0 rg CaCO3/L) 23 -5. 5 _ C.) v5o,01oo6 T 8 I S - wbS0 D6ii R JLL _ _ I~II~I~ _ 3I-45 44_____ I uzý -U 0 "11 I -, I- Uz~ U90c211 ~ ~ I F F -~
+ -~--~-- F -~---~- F -- '-- 4 ---------
+,-~4I~-I--.---f---~-.----f------4---------4 4
.- ~
L41ýýý 'I- 1 t+ý li - i +; - Nbe-w--.... . + -4 - 4
- '~ ALL -L 12-(3 t age - l Iffo'>1 5mli9-of titrantt is u j sa used, l emusttel sample u be diluted. Reviewed by:
h h-Date reviewed
! -15
-( 0,0t
Page E 9= Page -__* of, 4u19ln Total Hardness (SM 2340 C) RL 1.0 m CaCO3 /L Analyst[7!Z77.. ] . Time initiatec I Date analyzed L Ij- 13 ij Time completed [IiýII Titrantnormality and multiplierdetermination: J I TitraL_ Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference number stanuderd numbe -- (E)')ml ac ac = 0.2/1E 00
= 0-0.0220) (N x 50000)150
=Nx 1000 ml sample
[ ( Laboratory controlstandard: Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3 /L) (acceptable range (mg CaCO3IL) (ml) ml ml ml =90 to 110%)
~j$ ~40 10 4a2~o Duplicatesam le precision:
Sample Hardness %RFD = Sample Sample ID volume Begin End Total Multiplier (ing CaCO3IL) A(S - D) /[(S+D)/2]) x 100 number (ml) ml ml ml 10' _J _
* / 7Duplicate ý _ ( ).L_ _
.- 3 . ... D
- L "r7
______31 1_ 3.3 LU Matrix spike recovery: "__ Reference standard Spike value Sample Spike hardness (A) number (SV) volume 'Begin End Total Multiplier (mg CaCO3,L) (mg CaCO3/L) m m) ml ml Sample hardness (B) Measured spike value (MV) % R MV SV x 100 (mg CaCO 3/L) MV= A-B (acceptable range (mg CaCOIL) = 75 to 125%) Sample measurements: - Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mrg CaCO3 /L) TV--*g2_* Blank 0o01 01* -.O,* Av Z o 3*. -2 3"* 3. 20 D*(0 01hLlz car O's0 -LL 23~. j 1OS 4
.0-
.Q~1 _____ L-. ' 2 *4 2,2_5 o1osU,,o / 2. __ __._5 t4 _5C t-,.-a-f *fS Qt! I 0.0 A13 J
- 0 --1!1z&k- G i .,7-4 5 LAL-
. 4 * ~ L~~7 * ~ j. L.{L4 I'
I 4 1
. I 4
I. I-
~-,.-------4----~--- 4 .~ I os-OS-01NA I -~
1-1 U; 0
.13 ._4- I tLF5
[age 73 of t*7* If >15ml oftitrant is used, sample must be diluted. Reviewed by: . Ii I Date reviewed [ 0L D-01 1
Page To__ Page __. of C(.
'a S
- En lonmenteI Testlng Solution.. Inc.
Total Hardness (SM 2340 C)
'R= 1.0._Mg CaCO 3/L D a yste .... Time initiated Date analyzed , (-,c-10 ".oq Time completed I Titrant normality and multitwler determination:
Titran -k Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard In ml = 0.21E (N x 50000)/ 50 ml sample number number ce table range 0.0180 - 0.0220) = Nx 1000 Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS = MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (mg CaCO3 /L) (ml) mJ ml ml = 90 to 110%) 40 50 . .2- c. 2-0.0 4ot0" Duplicate sam le precision: Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (Ing CaCO3/L) {(S - D) /[(S+D)/21} x 100 number . (ml) ml ml ml . 00*,65OS-.'L avtt 2s *. __. . _-_
- t Duplicate (B) 4.9 q-2 -I ID r ~~
Matrix spike recovery: ." Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mng CaCO3 1L) (mg CaCO 31L) (ml) 0 ml ml F)S Stp t41-12 2-0Z Z Sample.hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO3/L) MV = A - B (acceptable range (mg CaCO3/L) 75-to 125%) Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID ( , ml ml ml Multiplier .(rg CaCOJL) TV =TVNDBln ~ ~= Do-uld be = 0 mn CaCO6/L)
.Au ,n-- Ft
*1i iqt 1- 35 4 , A 2c).o 110 orOs S.O\ , 0 00 AO3 2 ,_ 42 (y T 3.1 1, 0 o OS -161o. 0)I3i*i*iZI_ iL I
t'A~sC.OE. OR' 12,14 Il~~ ~WA~ _r _ _1__* _ I_2_ I[ 4).*
- t. I .L Jý gage 74 of Wo:if>15ml of titrant is used, sample must be diluted. Reviewed by: I I Date reviewed [ 0 -Is.0l{-
Page _'W Page _ of 4 innlonmenta Tmtin alurbtons.Inc. Total Hardness (SM 2340 C) RL = 1.0 mrg CaCO/L Analyst[ . 7J -- Time initiated F77771 Date analyzed j '-5j- j] Time completed EZI ._ Titrantnormality and multiplier determination: Tit Normality check Begin End Total Normality (N) of EDTA. pH Factor or Multiplier reference standArd- -a-r- --- mI- ml = 0.2/E = (N x 50000)/50 ml sample number number ac---aceptable range = 0.0180 - 0.0220) = Nx 1000 Laboratory controlstandard: .__ __ Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number '(TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO 3/L) (ml
- ml ml ml =90 to 110%)
Xl"6040 50aiVd0. 002 Duplicatesample precision: Sample Hardness %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO3JL) {(S - D) /[(S+D)/21) x 100 number (ml) ml ml ml OR Os 06-'LS 7VA UtP~ -?., -ý7 L,11 _____O Duplicate (B) *- * .- . ,___ i.Matrix spike recovery: _ Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3 IL) (mg CaCO3/L) (ml) ml ml ml Sample hardness (B) Measured spike value (MV) % R= MV / SV x 100 (mg CaCO 3/L) MV = A - B (acceptable range (rag CaCO3/L) =75 to 125%) Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L) TV=2DBlank Vg =.*' mCO/L). _OcsoS-Oc -#A6*--rnt . _,__._ 7-I.Co 4-o 001 0-506--Z( 2-~f~o ~ n0 3q _ ___5i 0__ re__ __ 2Sý __ fl4 pCiUSQ.I'0 -NA___ ~ ~ 1 _ __ _ _ _ _ _ Or,fln, t ii i 2 1.i 14's .314 - Z - 4 ++ I .1 "I .1- 4 - 4---.--.-.~~----i--~....-----i---..----------i l**t~~) g ngý 0e, nk. It I.- JV--)
- - - - 4 + +----f-~--+-~--'-'----4---------~ +
n CQq0: [/A W&V11Jl7~-W) Q YýL-Li i110 - 11L I I 0A2 ~III?~ It age 75 of Note:107If >l5nmd of titrant is used, sample must be diluted. Reviewed by: i I I reiw-/ Date reviewed] 0, 'iO - I*
Page 9_ _ Page of I Analyst andttderia Total Hardness (SM 2340 C) RL = 1.0:mg CaCO3 L Time initiated I] Date analyzed o -mi~iiiq Titrant normality and multiplier determination: Time completed LI *I iran I !End Total Normality (1) of EDTA pH Factor or Multiplier reference standard ml ml = 0.2/E (Nx 50000)/50 ml sample I number number (E) (accepta e - 0.0220) = NxI 1000 I Laboratory controlstandard. Reference standard True value Sample Hardness (MV) % RS = MV / TV x100 number (TV) volume Begin End Total Multiplier (mg CaCOj/L) (acceptable range ~i (mg CaCO3/L) (ml) ml ml ml =90 to 110%) o 0 ;
- 40 50 3 ~5 e. ~ 4 Duplicatesample precision: _
Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL) ((S - D) /[(S+D)/2]} x 100 I number O(6 01 I2' 4A/ )%P ILW I (ml) ml ml mln[ S ___ Duplicate.(B) D 4 Matrix spike recovery: ____ Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3 IL) (mg CaCO3IL) (ml) ml :ml ml Dý%(q J-4.14~ITh.' Sample hardness (B) Measured spike value (MV) % R MV / SV x 100 (magCaCO3/L) MV = A - B (acceptable range (mg CaCO3/L) = 75 to 125%) Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (mI) ml ml ml Multiplier (mg CaCO3IL) TV =,l)Blank 01_ 004 oo_.. N - _- ,4 2-09t 0ofo. 12-- :3 L201.o 131.9 :q,,3 fv? 92-
,,,A osoL, .o(. 7/A lU4~k~ijI2.,n-fdSl 1piq
'31q N.q 01 I I
qOS04 - of- 71A M- týdiirrfi)Jl 7 i 1): (Z, 3 Qri TI..)- kage 76 of WEo: If >15ml of titrant is used, sample must be diluted. Reviewed by: Date reviewed [ -P) .LfA .
Page '-ý' Page C,2. of(_O
-*,* rntalTIrg 5.lutions, Inc.
Envtonmn Total Hardness (SM 2340 C) RL = L1O,0mg CaCO3 /L Analyst Date analyzed [%,7.... Time initiated Time completed I I 1 TitraizLnozmalltv and multinlier determination: Titrant Normality.check Begin- -n Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml */500 N /50 ml sample number number (E) (acceptable range = 0.0180 - 0.0220) x 1000 Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS=MV/TVx100 number (TV) volume Begin End Total Multiplier (mg CaCO3IL) (acceptable range (mg CaCO3/L) (m4) mI ml ml
- 90 to 110%)
.13 ( ~- 40 50o 2o.0 -,o Duplicatesamr pie precision:"
Sample Hardness %RPD = Sample Sample ID volume Begin End Total Multiplier (mag CaCOfL) ((S - D) /[(S+D)/21) x 100 number (ml) ml ml ml o'io nusclo EI~iTn oo 145' 3Co 2- 3q 1 33.~-t LI Q S Duplicate (B) - - - , - " D / Matrix spike recovery: "____ Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (Mg CaCO3 L) (Mg CaCO 3/L) (ml) ml ml ml 4z.,,6 S (7) -'0b 0a Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mng CaCO3 /L) MV = A - B (acceptable range (mag CaCO 3/L) = 75 to 125%) Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L) TV = 14D- Blank _4L (should be = 0 mg CaCO3/L)
,)tfl.oL. EIY vp 001 5"0 Mo
)24 . i.A )-o.o O', osal. 1 T 0o2.j. /0 NA 1q
__ 5) 10 OS - O EM *0 J, 2 -3*,_ _-_--, if "'- _ __ __ _ __ _ _~~~~ _ _ _ _ _ _ i - 1 t~~t Sage 77 of IoTte: If >15ml of titrant is used, sample must be diluted. Reviewed by:t(,4j,. I1 Date reviewed Ii Orý3?iiI
Page ___M Yl Page :1- of 3 Total Hardness (SM 2340 C) RL = 1.0 mg CaCO 3/L Analyst bs... Time initiated [Dy:-i Date analyzed ' Time completed Iý,.S Titrant normality and multinlier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml = 0.2/E = (N x 50000)/50 ml sample number number (E) (acceptable range = 0.0180 - 0.0220) = Nx 1000 Laboratorycontrol standard: Reference number standard True(TV)value Sample* volume Begin End Total Multiplier Hardness (mMgCaCOVL) (MV) % RS = WV / TV (acceptable rangex 100 (Ing CaCO3fL) (ml) ml ml ml =90 to 110%)
-TWI6 40 50 i).0 12U- e,U, f40 Duplicatesam te precision: "__
Sample Hardness %RPD = Sample Sample ID volume Begin End Total Multiplier (nmg CaCO 3IL) {(S - D) /[(S+D)/2]) x 100 number O*2ti ji'-. (ml) ml I mlF 2.1..* ml S " - '* . .- .... S Duplicate (B) . - I =1,5 q . ,-Jt. . D C12- ,.*.*. : Matrix spike recovery:._ Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3JL)
.... _ .(mg CaCOj/L) (ml) ml ml ml
________ ______ 50 52 Q5 Io -to%) , Sample hardness (B) Measured spike value (MV) % R =MV / SV x 100 (mg CaCO 3/L) MV= A - B (acceptable range. (rmg CaCO3/L) =75 to 125%) 92- 3,1q5* Sample measurements:,- Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (rag CaCO3/L) TV=ND Blank 4**o ,g* **4*. o 6S.oqo9A- U L) -- 4 ,s OS *,**o _ -.0!' __ Fb'-4,OD-', -7 J __ ___ 4 tL.C 1ILq
. -- J I -1 I 'I-'
I ~S~L~zz I I -2~ t ~ +/-+/-_ I. ~=~= I 1-tge 78 of 10hte: If >15ml of titrant is used, sample must be diluted. Reviewed by: Date reviewedI Date1reiewed Z Q-f
"0 Page _q6 s
Page 3of 3
,*'EnvWrom'naIT~svingSo~utins, Inc.
Total Hardness (SM 2340 C) RL = 1.0 mg CaCO3/L Analyst f --. Time initiated. Date analyzed L.e-i* -II 'Tinme completed Titrantnormality and multiplier determination: I TR*rý -NQ-rmalitY check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standar- ------ - . ml ml = 0/E -(N x 50000)/50 ml sample I number number -- _._acceptable ( range= 0.0180 - 0.0220) = N x 1000\ I Laboratory controlstandard: . Reference standard True value Sample Hardness (MV) % RS = M / TV x 100 number (TV) volume Begin End Total Multiplier (Mg CaCOj/L) (acceptable range (rngCaCO3/L) (ml) ml ml ml =90 to 110%) 17%--f 40 50 Gt `6 4-' Q- 2D.0 ~ c Duplicatesam le precision:-.. Sample Hardness %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) {(S - D) /[(S+D)/2]} x 100 number (ml) ml mil ml oicsik-ol 7-A.swlo. ____) )4-1ý 19c, 3 2-D-O Duplicate (B) D Matrix spike recover:. Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L) (mg CaCO3/L) (ml) ml ml ml Sample hardness (B) Measured spike value (MV) % R =MV /SV x 100 (mg CaCO 3/L) MV= A - B (acceptable range (mrg CaCO 3/L) = 75 to 125%)
\o4-h Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (Ing CaCO 3/L) TV =N -t.- ' n ,k-(should be =0 rg CaCO __L) 15-os,*o 11 of A*. iq% .- 3o ocl*o¶.l?, o9 z 3~a.5 3,5,. .%i I 041O S -s . -3 - .Q- (1-) maotoiA- toto 4 39-,+ LL 3=. e 411__1 ~ Ift
-I II i.
- - - - - - -4 o0 ao% l .-- o PL4 J,%- j I ,__ b.t 3 3.3 lage 79 of 1 Wte: If >In5ml oftitrant is used, sample must be diluted. Reviewed by: - - - Date reviewed Dv5- nr+-
Page I"/I Page 3 of 3 Total Hardness (SM 2340 C) RE = 1.0 mg CaCO3 /L Analyst [g_ . Time initiated Date analyzed LI Time completed Laboratorv control standard: Reference standard True value Sample Hardness (MV) % RS = MV / TVx 100 number (TV) volume Begin End Total Multiplier (rag CaCO3/L) (acceptable range (rag CaCO3/L) (ml) ml ml ml 90 to 110%) ING:S. WtoS 40 50 3.3 5".-4 C. 2-73 ýO i5C-L lb Duplicatesam ple precision: Sample Hardness %RPD = Sample Sample ID volume Begin End Total Multiplier (rag CaCO3 /L) {(S - D) /[(S+D)/21) x 100 number -'_[ (ml) ml ml ml ClqO~lb-,oi 15(aýtYOQ 2- 6-D 5.*4 .5 3.t1 .. .* .-**,** Duplicate (B) " [ g * *.'4 * ".- *D V4.','.-*f2 Matrix spike recovery: __ Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOVL)
.(mg CaCOVL) (ml) ml ml ml 0 5D-- ,.5 17, q- .aIC)0 Sample hardness (B) Measured spike value (MV) % R = MV / SV x. 100 (rag CaCO 3IL) MV.= A - B (acceptable range (mg CaCO 31L) = 75 to 125%)
Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (m) " ml ml ml Multiplier (rmg CaCO3 /L) T=NDBlank.I-TV__= ___(should be = 0 mg CaCO 3IL)
-#ooSo,. -i*. LX ..D I g z --
oios(~.I4 ~ . 4~ l~ (' _____ ________ 4- f-=-~---4 4 5 4.
- I I-4 *4 4 4- -. L ~ -4 _______________________ _______________ I .h.. ....L I t
ge8ol~i~e: ge 80 of If >l5ml oftitrant is used, sample must be diluted.
,, o. V Reviewed by: Z. i.__ Date Date rreviewed e: **0*
Sequoyah Nuclear Plant Biomonitoring May 12-19,. 2009 Appendix D Reference Toxicant Test and Control Chart Page 81 of 107
Pimephales promelas Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc. Environmental Testing Solutions, Inc. 19 .. . I.* I I I I I I I I I I I I I i i i USEPA ControlLimits (+/- 2 StandardDeviations) 1.0 F-0.8 0.6 0.4 I I I I ; 1 1 1 I 1 I1 ' I. I I I I I I I 1.2 I W arnII I I I I I I I I I I I I USEPA .Warningand ContivotLimits (7,5 ,,and 9 Oth Percentile CVs).... 1.0 Qn 0.8 eq 0.6 P=- 0.4 H ...........I . . .... .........I...........I .......I........ ............... . lcI I I I I I ~ I I I I I I I I I I I 1.2 I l I i I I I I I I I I I I I I I Laboratory Warning and Control Limits. (10 and 25 PercentileCVs) 1.0 0.8 i 0.6 0.4 S I I I I I i I I I I I . *i I Test date 7-day IC2 s = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population. Central Tendency (mean IC 25 ) Warning Limits (mean IC2 5 - SA1 0 or SA.75) Control Limits (mean IC2 5 +/- SA.251 SA 90, or 2 Standard Deviations)
Pimephalespromelas ETS Chronic Reference Toxicant Control Chart 4 Environmental Testing Solutions, Inc. State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day IC . CT S Control Limits S&.0 Warning Limits SA.2 Control Limits SA.7 Warning Limits SA.tO Control Limits CV 5 (&'L KCI) (gfl, KCI) CT - 2S CT+2S CT-SAt.1 CT+SA.10 CT - S*2 5 CT + SA.as CT - SA75 CT + SA.s CT - SA.,o CT + S,90 i 10-28-08 0.79 2 11-04-08 0.67 0.73 0.08 0.56 0.90 0.09 0.64 0.82 0.15 0.58 0.88 0.28 0,45 1.01 0.33 0.40 1,06 0.11 3 12-09-08 0.69 0.72 0.06 0.59 0.84 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.39 1.04 0.09 4 01-06-09 0.76 0.73 0.05 0.62 0.84 0.09 064 0,81 0.15 0.57 0.88 0.28 0.45 1.00 0.33 0.40 1.05 0.07 5 01-13-09 0.82 0.75. 0.66 0.62 0.87: 0.09 0.66 0.83 0.16 0.59 0.90 0.28 0.46 1.03 0.34 0.41 1.08 0.08 6 01-15-09 0.77 0.75 0.06 0.64 0.86 0.09 0.66 0.84 0.16 0.59 0.91 0.29 0.47 1.04 0.34 0.41 1.09 0.08 7 01-21-09 0.78 0.75 0.05 0.65 0.86 0.09 0.66 0.85 0.16 0.60 0.91 0.29 0.47 1.04 0.34 0.42 1.09 0.07 8 02-03-09 0.79 0.76 0,05 0.66 0.86 0.09 0.67 0,85 0.16 "0,60 0.92 0.29 0.47 1.05 0.34 0.42 1.10 0.07 9 02-10-09 .0.83 0.77 0.05 0.66
- 0.87 0.09 0.67 0.86 0.16 0.61 0.93 0.29 0.48 1.06- 0.34 0.42 1.11 0.07 10 02-17-09 0.53 0.74 0.09 0.56 0.92 0.09 0.65 0.83 0.16 0.59 0.90 0.28 0.46 1.02 0.33 0.41 1.08 0.12 11 02-24-09 0.60 0.73 0.10 0.54 0.92 0.09 0.64 0.82 0.15 0.58 0.88 0.28 0.45 1.01 0.33 0.40 1.06 0.13 12 02-25-09 0.76 0.73 0.09 0.55 0.91 0.09 0.64 0.82 0.15 0.58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.12 13 02-27-09 0.72 0.73 0.09 0.56 0.91 0.09 0.64 0.82 0.15 0;58 0.88. 0.28 0.45 1.01 0.33 0.40 1.06 0.12 14 03-03-09 0.75 0.73 0.08 0.56 0.90 0.09 0.64 0.82 M 0.15 0.58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.11 15 03-10-09 0.79 0.74 0.08 0.57 0.90 0.09 0.65 0.82., 0.15 0.58 0.89 0.28 0.46 1.02 0.33 0.41 1.07 " 0.11 16 03-10-09 0.77 0.74 0.08 0.58 0.90 0.09 0.65 0.83 0.16 0.58 0.89 0.28 0.46 1.02 0.33 0.41 1:07 0.11 17 04-14-09 0.70 0.74 0.08 0.58 0.89 0.09 0.65 0,82 0.15 0.58 0.89 0.28 0.46 1.02 0.33 0.40 1.07 0.11 I8 04-21'-09 0.80 0.74 0.08 0.59 0.89 0.09 0.65 0.83 0.16 0.58 0.90 0.28 0.46 1.02 0.33 0.41 1.07 0.10 19 05-05-09 0.69 0.74 0.08 0.59 0.89 0.09 0.65 0.83 0.15 0.58 0.89 0.28 0.46 1.02 0.33 0.41 1.07 . 0.10 20 05-12-09 0.75 0.74 0.07 0.59 0.89 0.09 0,65 0.83 0.15 0.58 0.89 0.28 0.46 1.02 0.33 0.4] 1.07 0.10 Note: 7-d ICz5 = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
CT = Central tendency (mean IC25 ). S = Standard deviation of the IC 25 values. Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and.warning limits recommended by USEPA for the test method and endpoint. SA.15= Standard deviation corresponding to the I0h percentile CV. (SAlo = 0.12) SA.5s= Standard deviation corresponding to the 25"' percentile CV. (SA 2s= 0.21) USEPA Control and Warning Limits S,75 = Standard deviation corresponding to the 75"' percentile CV. ( SA.75= 0.38) S.,O =-Standard deviation corresponding to the 90"h percentile CV. (SA.0 = 0.45) CV= Coefficient of variation of the IC25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applicalions Under the National Pollutert Discharge Elitnination Program. EPA-g33-R-00-O03. US Enviroamennntal Protection Agency, cimrw i, OH. Organismsobtaimfdtfrom Aquaio, Inc. atox05-12-09
- ~ Precision of Endpoint Measurements
, "IPimephales I" Chronic Reference Toxicant Data promelas
- Environmental Testing Solutions, Inc.
Control Control Mean Test number Test date Survival CT CV CT MSD PMSD CT Growth for Control Growth for Control (%) (mg/larvae) (mg/larvae) (%) for PMSD (%) Growth CV (%) 1 10-28-08 100 0.586 6.5 0.10 17.8 2 11-04-08 97.5 0.643 0.614 2.5 4.5 0.12 18.7 18.2 3 12-09-08 100 0.522 0.583 2.4 3.8 0.05 10.0 15.5 4 01-06-09 100 0.644 0.599 3.2 3.6 0.09 13.3 14.9 5 01-13,09 97.5 0.556 0.590 9.7 4.9 0.06 11.2 14.2 6 01-15-09 100 0.707 0.610 6.5 5.1 0.09 13.3 14.1 7 01-21-09 97.5 0.594 0.607 4.9 5.1 0.07 11.5 13.7 8 02-03-09 100 0.671 0.615 8.4 5.5 0.06 9.6 13.2 9 02-10-09 .100 0.591 0.613 5.1 5.5 0.09 15.7 13.5 10 02-17-09 100 0.804 0.632 3.3 5.2 0.06 6.8 12.8 11 02-24-09K 100 0.744 0.642 2.5 5.0 0.10 13.0: 12.8 12 02-25-09 100 0.659 0.643 8.2 5.3 0.07 11.1 12.7 13 02-27-09 100 0.578 0.638 6.8 5.4 0.08 13.1 12.7 14 03-03-09 100 0.664 0.640 8.1 5.6 0.08 12.3 12.7 15 03-10-09 100 0.661 0.642 6.8 5.7 0.09 12.9 12.7 16 03-10-09 100 0.696. 0.645 8.8 5.9 0.09 12.6 12.7 17 04-14-09 100 0.671 0.646 10.3 6.1 0.08 11.4 12.6 18 04-21-09 100 0.642 0.646 8.3 6.2 0.07 11.2 12.5 19 05-05-09 100 0.805 0.655 7.9 6.3 0.07 8.4 12.3 05-12-09 100 0.717 0.658 7.0 6.4 0.06 8.9 12.1 20 Note: CV= Coefficient of variation for control growth'. Lower CV bound determined by USEPA ( 10th percentile) = 3.5%. Upper CV bound determined by USEPA ( 9 0 th percentile) = 20% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10n percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile) 30%. CT Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtainedfrom Aquatox, Inc. atox05- 12-09 Page 84 of 107
"ETS Environmental Testing Solutions, Inc.
1.25 I I Pimephalespromelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source: Aquatox, Inc.
-S 1.00 0.75 0
0.50 0.25 USE] I I I I 1
-. - I I I I I I I I I 'I I I I I I I I I I 30 U Kentucky Acceptance Limit (< 30.0%)
A Cu C 0 20 I. 0* a-1.0 o.J 0 0 .. ...
..... .......... ....... ...................1..
30 .. USEPA Upper PMSD Bound (90"' percentile <30.0%) 20 ~~ ~ ~ .. "............... .... 20 .0+............. ......................................... 10 It Ie I I I I I Test date
---- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
-- -- Central Tendency (mean Control Growth, CV, or PMSD) ige 85of i 0 7Control Limits (mean Control Growth, CV, or PMSD + 2 Standard Deviations)
-j
r 6 9 Page I of 5 DSETS Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas PpKCICR Test Number: Dilutionpreparationinformation: Comments: KCI StockINSS number: It,ýS 6'k__ Stock preparation: 50 g KCVL: Dissolve 50 g KCI in I-L Deionized water Dilution prep (mg/L) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume.(mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Test information: Organism age: "o. , . 0
- Randomizing template: I(e.2.
Date and times organisms ' - o\ 0 boo Incubator number and were born between: shelf location: Organism source: fkmý 6 e oo* Cs_0%. o, , Artemia CHM number: (A 4Lqq Transfer bowl information: pH = SU Temperature = C Date / Time in: 0 S-. jg- __q
-1'. -4.-o Date/Time out:o-. 20-dk kZZo Average transfer volume: Total drying time: Z4. .S4OJwC
- 0. 1"WC)S Initial oven temperature: 60"C, Final oven temperature: hi" ,..L Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW or termination batch used Time Analyst Time Analyst Time Analyst 2 os,-,A. eqto 1o OS-o 0 1 6 .L 2 O aL.. 1q1 C)t, 0 IL a is'.L 1 6___OS-11-po L1. %Soo Os 1LZ nt ' 6kA 'id-n 2..mS11
.1 OS-Iqi -o0 lS6 7
Control information: Acceptance criteria Summary of test endpoints:
% Mortality: 0)7. _20% 7-day LC50 9qq. 0 Average weight per initial larvae: _ 0,-11'"= NOEC (Vbo Average weight per surviving larvae: 011l "1 2Ž0.25 mg/larvae LOEC - SO ChV t,,'3 O. 8 IC25 5. ,
ge 86 of 107
lETS Page 2 of 5 Species: Pimephalespromelas PpKCICR Test Number: j_2-Survival and Growth Data Day Control 450 m KCI/L 600 mi KCI/L A B C D E F G H I J K L 0 /Q0 to to/Q /0 to0 10 to t /0 1C) I0 tO0 c /0 /0 /0 16 1) (3 ./0 /0 tO 2 10 '0 /0 O 0 /0 /0 /0 16( /1 /0 3 ,O /0 1( O 11) ! I /0 /
/0 0 /0 /0 4f /0 O /0 t0 1 0 /0 5
50 I /0 to 10 1 1O)/0 /0 /0 /0, /0 6 0 tO iO 10 (0 (O t/0 10 0 10 /0 10 7/ /0o 10to 1 e0 o 0t0 0 A = Pan weight (mg) Tray eolor code:: or v**_ . 14 -.11 13.&-ý 143,3 13.oD " 14.49 14 ,3T I}.3 1 .9 i t I . " .q ;q Analyst: _ npli Date: O-ofq;:-A'__ B = Pan +.Larvae weight Analyst: **1 Date: bS*___11__ - Oak C = Larvae weight (mg)
=B-A Weight per initial number I,/.
'4, ~1 S. "2 N of larvae (mg) 'V 0% tv
= C / Initial number of larvae .3 N N O" N-z N Qa 0 '-V V 0' Average weight per initial Percent reduction from control 0111
-1 -
O~bbq,.*'l .110 j I.O7." number of (%) larvae (mag)_ _ I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk Ig = unusually large, d&r decanted and returned, w wounded. sick, sm = unusually small, Calculations and data reeviewed: Comments: age 87 of 107
Page 3 of 5 D, Species: Pimephalespromelas PpKCICR Test Number: 2s 2-Survival and Growth Data Day 750 mg KCVL 900 ma KCIL 1050 ml KCI/L M N O P R S T U V W X 0 to /l t0 / 0 /0 /0 i( /D /0 /0 /0 1 sk qA' S q4. s,( s. 3x f
' 6' (0 /0 1 to -1 S S 1 1.
2l Ib
- n *to b o *S * *'
( 05*'* .10k k, _5 S _ LA lo 6 ilk~~ 5 . '~ ' 74 A Pan weight (rmg) t ray eolorcode:i O*r! 4.4q jtj.a iqj ' j -g l1 .01 13 -6ý 3 1 14 -74 1;.-64 4 Ab11~g 4,11 1*; J* 1 1,13 Analyst: - ,roLI Date: os -"' -A*" B - Pan + Larvae weight (Mg)
.Analyst: At,".r .9 1qg t ~O.,'o K58 1i.qs )6.11 l1-.3" WoS6 )bL-t it13q IV443 )-4-6 Date: 054* -o0
-_q C - Larvae weight (mg)
=[If-A ,3:bq 5 5.5 .5" -.*53 3.-00 2-5.* ,, .Cz. z.*q *. zg* 2 s Weight per initial number ",,o oflarvae (mg) 3' \0 0
= C/Initial number of larvae Average Percent weight per reduction initial from control 02... IS 0.,'34 5 S &Z O.2TIT.S.
number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk = sick, sm. unusually small, Ig unusually large, d&r = decanted and returned, w.= wounded. Calculations and data reviewed: Comments: lage 88 of 107
00 O0 "-,4 Pimephalespromelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
- Environmental Testing Solutions, Inc. Test number: PpKCICR#182 Test dates: May 12-19, 2009 Reveawed by:
Coacetration(mg/L Rtplicat Iialrnbcrof le FI..alnmbeeof A-Panwaigkt(mgl B-P.,+La-vae Larv-eweigbt(ag) Waghtl/Srvivi.z Meweight/ Co ttofnatofvio*I Weigkillaitialaltmber Mea.arvival Mea weight/Initial 'm*"fof Pe-1 redscuosfrumn Ka) larvae larv( (dg.ttog) =A-B aumbherflurvaelmg) Sorvivingnualber - of larvae(mg) .(%) .mberof larvae varitoa (%) ootr.l (%) A 10 10 14.77 21.46 6.69 0.669 0.669 Control B 10 10 13.07 20.29 7.21 0.121 0.717 7.0 0.721 100.0 0.317 7.0 Not applicable C lQ 10 14.33 22.18 7.85 0.785 0.785 D 10 10 13.05 19.97 .6.92 0.692 0.692 E 10 10 14.49 21.61 7.12 0.712 0.712 450 F 10 10 14.27 20.79 6.52 0.652 0.686 3.8 0.652 97.5 0.669 67 6.7 G 10 10 13.31 20.29 6.98. 0.698 0.698 H 10 9 14.89 21.03 6.14 0.682 0.614 I 10 10. 13.41 20.82 7.41 0.741 0.741 680 1 10 t0 12.75 19.93 7,18 0.718 0.710 3.6 0.718 K 10 10 15.43 22.26 6.83 0.683 . 0.683 100.0 0.710 3.6 1.0 _L I0 10 12.93 19.90 6.97 0.697 0.697 1 M 10 8 14.44 19.90 5.46 0.683 0.546 750 0N 10 10 88 14.92 14.85 19.87 20.20 4.95 5.35 0.619 0.669 0.667 5.1 0.495 0.495 80.0 0.533 5.1 256 P 10 8 14.01 19.58 5.57 0.696 0.557 Q 10 6 13.62 17.95 4.33 0.722 0.433 900 R 10
- 5 13.11 16.11 3.00 . 0,600 0.654 7.7 0.300 52.5 0.345 23.6 51.8 S 10 4 14.76 17.32 2.56 0.640 0.256 T 10 6 12.64 16.56 3.92 0.653 0.392 U 10 3 14.23 16.47 2.24 0.747 0.224 V 10 4 14.11 17.39 3.28 0.820 0.733 110 0.328 275 61.7 1050 W 10 4 13.81 16.77 2.96 0.740 0 0.296 37.5 0.7 169 X 10 4 15.13 17.63 2.50 0.625 0.250 1 Dunnett's MSD value: 0.0640 MSD = Minimum Significant Difference PMSD: 8.9 PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the sinsimum percent difference between the control and treatment that can be declared statistically sigmficant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 12%. Upper PMSD bound determined by7USEPA (90th percentile) = 30%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Intedaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 200 Ia, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-82I-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtainedfom Aquatox, Inc. atox05-12-09
9 0 0 Statistical Analyses -5 :FTS 0 SEnvironmental TestingSolutions, Inc. -. 1 Larval Fish Growth and Survival Test-7 Day Survival Start Date: 5/12/2009 Test ID: PpKCICR Sample ID: REF-RefToxicant End Date: 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mg/L I 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 0.9000 600 1.0000 1.0000 1.0000 1.0000 750 0.8000 0.8000 0,8000 0.8000 900 0.6000 0.5000 0.4000 0.6000 1050 0.3000 0.4000 0.4000 0.4000 Transform: Arcsin Square Root Rank I-Tailed Number Total Cooc-mg/L Mean N-Mean Mean Min Max -, CV% N Suns Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 450 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 16.00 10.00 1 40 600 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 .A10.00 0 40
*750 0.8000 0.8000 1.107.1 1.1071 1.1071 0.000 4 10.00 10.00 8 40
*900 0.5250 0.5250 0.8106 0.6847 0.8861- 11.892 4 10.00 10.00 19 40 1050 03750 0.3750 0.6584 0.5796 0.6847 7.979 4 10.00 10.00 25 40 Auxiliary Tests Statistic Critical Skew Kurt .
Slapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) 0.81382352 0.884 -1.2632773 2.14787125 Equality of variance cannot be confirmed Hypothesis Test (I-tail, 0.05) NOEC LOEC ChV TU-Steel's Many-One Rank Test 600 750 670.820393 Treatments vs D-Control Maximum Likelihood-Probit Parameter Value SE 95% Fiducial Limits Control Chi-Sq Critical P-value Mu Sigma Iter Slope 8.14828973 1.29114829 5.61763904 10.6789404 0 6.87582293 7.81472778 0.08 2.97727854 0.12272514 4 Intercept -19.259728 3.78378062 -26.675938 -11.843518 TSCR Point Probits mgL 95% Fiducial Limits ECOI 2.674491.787145 380.202898 566.020355 EC05 3.355 596.229761 500.029379 659.146411 ECIO 3.718 660.693371 577.154041 716.766606 ECI5 3.964 708.079653 634.372816 760.17228 EC20 4.158 748.153069 682.308654 798.356768 EC25 4.326 784.335178 724.514185 834.704503 EC40 4.747 883.459176 829.997827 948.216155 EC50 5.000 949.026949 890.476916 1035.58115
.EC60 5.253 1019.46098 949.626271 1137.82792 EC75 5.674 1148.30007 1049.26122 1340.14047 EC80 5.842 1203.83407 1090.1848 1431.97553-EC85 6.036 1271.96449 1139.27255 1547.85189 EC90 6.282 1363.19229 1203.47434 1708.07612 EC95 6.645 1510.57893 1304.3074 1978.13621 EC99 7.326 1831,38628 1514.60449 2608.95733 Organisms obtainedfrom Aquatox, Inc. atox05-12-09
)
Statistica1 Analyses
'* Environmental Testing Solution s, Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/12/2009 Test ID: PpKClCR Sample ID: REF-Ref Toxicant End Date:. 5/19/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mg/L 1 2 3 4 D-Control 0.6690 0.7210 0.7850 0.6920 450, 0.7120 0.6520 0.6980 0.6140 600 0.7410 0.7180 0.6830 0.6970 750 0.5460 .0.4950 0.5350 0.5570 900 0.4330 0.3000 0.2560 0.3920 1050 0.2240 0.3280 0.2960 0.2500 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7168 1.0000 0.7168 0.6690 0.7850 7.008 4 0.7168 1.0000 450 0.6690- 0.9334 0.6690 0.6140 0.7120 6.687 4 1.627 2.180 0.0640 0.6894 0.9618 600 0.7098 0.9902 0.7098 0.6830 0.7410 3.567 4 0.239 2.180 0.0640 .0.6894 0.9618 750 0.5333 0.7440 0.5333 0.4950 0.5570 5.070 4 0.5333 *0.7440 900 0.3453 0.4817 0.3453 0.2560 0.4330 23.589 4 . . 0.3453 0.4817 1050 0.2745 0.3830 0.2745 0.2240 0.3280 16.925 4 0.2745 0.3830 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97055012 0.805 0.28149786 -0.6879762 Bartlett's Test indicates equal variances (p 0.55) 1.1904434 9.2103405 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test -600 >600 0.06396215 0.08923913 0.00265975 0.00172172 0.2649911 2,9 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95% CL(Exp) Skew IC05 608.13 149.72 0.00 651.16 -0.8286 IC10 642.56 54.75 277.41 687.07 -3.5954 IC15 676.99 19.21 608.59 724.00 -0.4242 IC20 711.43 18.63 652.82 760.37 -0.2084 IC25 745.86 17.03 695.14 791.43 / -0.2232 IC40 832.34 23.14 784.14 931.75 0.9228 iC50 889.53 36.16 825.86 1033.39 0.8216 Organismsobtainedfrom Aquatox, Inc. atox05-12-09
F6 a DaT Page 4 of 5 Species: Pimephalespromelas PpKCICR Test Number: "&2. I Daily Chemisiry: Analyst //IIrt^ 0 MLr" Day, 12 L t'L.h
- f. L f-- hL Concentration Parameter CONTROL pHDO3
,S.U.) 6 "mgL-6 75 ",
7t .3 Conductivity __limhos/cm) ' 'r I.* 3fa 7i6 Alkalinity (mg CaCo 3*L) t) Hardness (mg CaCO 3/L) Temperature k - pH (S.U.) "3..1 "/77 75 _____ t,*8 -3"1 '4.32-. DO (mg/L) 4. 3 450mg KCIIL Conductivity J 0 1 Temperature 1 _gmhos/cm) Temperature DO (mg/L) 900 mg KCVL Conductivity Temperature1 7.-LN.. (gmrho2/cm) )~7 750 mg KCi/L Conductivity PH (s.u .) i
-__. _____"_- 1 -
Temperature .,t. "q pH(S.C) 2q- 3. 27- 5 -7 pH (S.U.) -41 ~-q9 I 4.3a -I-Tb) I SDO (mg/L) 1050 mg Conductivity - KCVL (umhos/cm) -ntl. 7 -Fia xn. i -ntq b Fin Temperature (CC) STOCK Conductivity _j (mhscm) I Initial. ia Initial Final. Initial Final Page 92 of 107
lETS Page 5 of 5 SpEnvcies: T.Pme.n5tirep p Species: Pimeplhalespromelas PpKCICR Test Number: t&L- __Day 3 - 4 5 6 Analyst Ii-r Aoda1 L Concentration Parameter CONTROL pH (S.U.) 14it 31~. DO (mg/) . -7.e8 Conductivity Alkalinity (mg CaCO 3IL) ý Hardness 89 (mg CaCO3 /L) Temperature.. (-c) --. A --A.- --. pH (S.U.) DO (mg/L) J 1* 7.38 t~q to Th7 . 7T*m_,/
-* . .1im
_5.."" 450.mg KCI/L Conductivity (*ýmhos/cm) i OI5 1 1 "z5 Temperature -LA4 OAS 24. 2'q 0 ________ ( c)_ _ _ pH (S.U.) 7.'a
-41 "i.I *7 , , . L U. "
60 0 mg KCV/L Conductivity Temperature .- q. C . - L- -I. _____ _____ (OC) _pH (S.U.) *r,Uo -. q " * * . 7 te -- / *~ * " " - l 750 mg KC/L DO (mg/L) '} -3 1 - _. - Conductivity (pmhos/cm). _' bZ 0 _/ I 9/ Temperature(
) .. 2 .1 -'"* .. L ." q. "£ " "
pH (S.U.) -7,L 4.0.O 7. (P,5 ,7, _ ý _ ' - ". b DO (mg/L) -" . "L -, 7-- ". -t4 ,,a 900 mg KCI/L Conductivity Temperature 2 S "24-I q .,- V T-24 -I "4 .. -- (0c ) oH,(S.U.) V, II Tq.' 7.' O 7-CoG -7,01 -'H.q I 4 -4. ' DO (mg/L) 1050 mg Conductivity KCI/L (j..mhos/cm) I Temperature (0C) Fn 1n.a naa ni STOCK 1 Conductivity I (jImhos/cm) Initial Final Initial Final Initial Fnl Initial Fi l a toe 93 of 107
0 Ceriodaphniadubia Chronic Reference Toxicant Control Chart
*= EnvironmentalITesting Solutions, Inc.
I I I I I I I I I SI t n I r I I I I I I I 1.14 -[ USEPA Control Limits*(J= 2 StandardDeviations) 1.12 - 1.10 1.08 1.06 1.04 ' 1.02 I I I I I II I I I I I I I I I I I 95, 25 USEPA Warning and Control Limits ( 7 5 th and 9 0th Percentile CVs) Q 2.0 1.5 1.0 Cu 0.5" ... ..I. I-1.4 I I !I I I ! i I " I I I I I I I I I Laboratory Warning and ControlLimits (10/h and 2 5 th PercentileCVs) 1.3 1.2 1.1 1.0 " 0.9 . .. ........ .. ...... .. . .. ...... . ... I I I I II I I I I I I I I I ! I I I I 0.8 z% %%Q's "%o Z%z% z% Z-- z% ".. zs "..ý'e- 6'V'q a"".) 'aq "q 'p QNO QN0 Q;O, 0", o"', zs* q6ý6'- 00, "0' losl- ý01 "N-Z' zip 03 q0"' Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population. Central Tendency (mean IC2 5) Warning Limits (mean IC25 + SA10 or SA.75) Control Limits (mean IC25 SA.25' SA-90, or 2 Standard Deviations) P age 94 of 4 07
Ceriodaphniadubia Chronic Reference Toxicant Control Chart
" Envir( nmntal Testing Solutions, Inc.
State and USEPA Laboratory Laboratory USEPA USEPA Test number Tt date 7-day IC, CT S Control Limits SAle Warning Limits SA-25 Control Limits SA.s5 Warning Limits SA-90 Control Limits CV (g/L NaCI) (g/L NaCl) CT-2S CTl2S CT - SAJO CT + SnA- CTr-s .CT + S&25 CT - SA.SS C" -SA.7s CT - S,, CT + S ,. 1 01-08-08 1.09 2 01-08-08 1.09 1.09 0.00 1.09 1.09 0.09 1.00 1.18 0.19 0.90 1.28 0.49 0.60 1.58 0.68 0.41 1.77 0.00 3 0.05-08 1.07 1.08 0.01 1.06 1.11 0.09 1.00 1.17 0:18 0.90 1.27 0.49 0.60 1.57 0.67 0.41 1.76 0.01 4 0104-08 1.07 1.08 0.01 1.06 1.10 0.09 0.99 1.17 - 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.01 5 04-08-08 1.07 , - 1.08 0.01 1.06 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.49 0.59 1.56 0.67 0.41 1.75 0.01 6 06-08 106 1.08 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 .0.01 7 .03-08 1.11 1.08 0.02 1.05 1.11 0.09 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.02 8 0708-08 1.07 1.08 0.02 1.05 1.11 009 0.99 1.16 0.18 0.89 1.26 0.49 0.59 1.56 0.67 0.41 1.75 0.01 9 0 5-08 1.09 1.08 0.02 1.05 1.11 0.09 0.99 1.17 0.18 0ý90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.01 O 0 09-08 1.10. .08 002 1.05 1.11 0.09 1.00 1.17 0.18 090 127 0.49 0.59 1.57 0.67 0.41 1.75 0.01 I1 1 07-08 1.09 1.08 002 1.05 1.11 0.09 1.00 1.17 0.18 0.90 1.27 0.49 0.60 1.57 0.67 0.41 .1.75 0.01 12 11 04-08 1.07 1.08 0.01 1.05 1.11 0.09 1.00 1.17 0.18 0.90 1.27 0.49 0.59 1.57 0.67 0.41 1.75 0.01 13 11 04-08 1.10 1.08 0.01 1.05 1.11 0.09 1.00 1.17 0.18 0.90 1.27 0.49 0.60 1.57 0.67 0.41 1.75 0.01 14 14,-02.08 1.106 1.08 0.02 1.05 1.11 0.09 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 0.01 15 01.06-09 1.05 1.08 0.02 1.05 1.11 0.09 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 002 16 02-03-09 1.05 1.08 0.02 1.04 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.75 0.02 17 0224-09 1.07 1.08 0.02 1.04 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.02
-18 03-10-09 1.07 1.08 0.02 1.04 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.02 19 04-14-09 1.09 108 002 1.04 1.11 .0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.75 0.02 20 05205-09 1.07 1.08 0.02 1.04 1.11 0.09 0.99 .1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.02 Note. 7 d IC25 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
CT = Central tendency (mean IC25). S = Standard deviation of the IC2, values. Lab ratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC1 5 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. SA I = Standard deviation corresponding to the I0 w percentile CV. (SA O = 0.08) SA.*5 = Standard deviation corresponding to the 25'h percentile CV. (SA2S =0.17) USE A Control and Warning Limits SA.sS= Standard deviation corresponding to the 75" percentile CV. (SA.75 = 0.45) SA.A9 = Standard deviation corresponding to the 900' percentile CV. (SA.m = 0.62) CV = Coefficient of variation of the IC2, values. USEPA. 2000 Und rstanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. 05-05-09
Precision of Endpoint Measurements Ceriodaphniadubia Chronic Reference Toxicant Data
') Environmental Testing Solutions, Inc.
Test Control Control Mean T Survival Reproduction CT CV CT MSD 'PMSD CT number for Control Mean for Control (%), (offspring/female) Reproduction (%) Reproduction (%) for PMSD (%) (offspring/female) CV (%) 1 01-08-08 100 30.3 6.6 2.2 7.3 2 01-08-08 100 31.8 31.1 4.9 5.7 2.4 7.7 7.5 3 02-05-08 100 31.3 31.1 6.4 6.0 2.8 9.1 8.0 4 03-04-08 100 31.5 31.2 8.0 6.5 .2.7 8.5 8.1 5 04-08-08 100 29.6 .30.9 7.7 6.7 2.6 8.8 8.3 6 05-06-08 100 32.1 31.1 6.3 6.6 2.5 7.9 8.2 7 06-03-08 100 30.5 31'0 7.9 6.8 3.0 9.9 8.4 8 07-08-08 100 30.9 31.0 6.7 6.8 2.4 7.7 8.4 9 08-05-08 100 29.4 30.8 7.9 6.9 2.2 7.6 8.3 10 09-09-08 100 28.3 30.6 8.5 7.1 3.0 10.5 8.5 11 10-07-08 100 32.4 30.7 7.2 7.1 2.7 8.4 8.5 12 11-04-08 100 32.3 30.9 5.1 6.9 2.2 6.8 8.3 13 11-04-08 100 31.5 30.9 6.2 6.9 2.4 7.7 8.3 14 12-02-08 100 31.5 31.0 4.6 6.7 2.5 8.0 8.3 15 01-06-09 100 33.2 31.1 4.0 6.5 2.0 6.1 8.1 16 02-03-09 100 31.4 31.1 6.6 6.5 1.9 6.0 8.0 17 02-24-09 100 32.2 31.2 6.0 6.5 1.8 5.7 7.9 18 03-10-09 100 31.9 31.2 5.8 6.5 2.6 8.2 7.9 19 04-14-09 100 33.9 31.4 6.7 6.5 2.0 5.8 7.8 20 05-05-09 100 33.6 31.5 4.3 6.4 2.3 6.8 7.7 Note: CV Coefficient of variation for control reproduction. Lower. CV bound determined by USEPA (10 h percentile) = 8.9%. th Upper CV bound determined by USEPA (90 percentile) = 42% MSD =.Minimum Significant Difference PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (I 0th percentile) = 13%. Upper PMSD bound determined by USEPA (90th percentile) 47%. CT =.Central Tendancy (Mean Control Reproduction, CV, or PMSD) ( USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. P05-05-09 Page 96 of 107
Ceriodaphniadubia
""ni
*D Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements
')Environmental Testing Solutions, Inc.
40 -. I I I . I I I I I I I I I I I I I I I I I - 35
~30
*' 25 S 520 U 15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female)
S I I I I I I ) I I I I I I~
-- - I I I -I - I -I -I I I 40 North Carolina Acceptance Limit (< 40.0%)
~30 Kentucky Acceptance Limit (< 30.0%)*
~20 Q10 42
- I I I ~ I I I I I I I I I I I I I I I I 50 T - I I I I I I I I I I I I I I I I USEPA Upper PMSD Bound (90°" percentile < 47.0%)
40 30 C,' 20 10
- I .1. I I I I I I I ....
A' Ll Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PNISDI )PMSD-"iz~ennm- saigcn~w differe~nce-between the con-tro and-treatmemnt-htcnb declared statistically significant. ill
-- Central Tendency (mean Control Reproduction, CV, or PMSD) ige 97 f 1 07Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
Page 1 of 6 F an.Tý 5-hn
- L-Sodium Chloride Chronic Reference Toxicant-Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphniadubia CdNaCLCR #: 80t Dilutionpreparationin ormation: Comments: NaCI Stock INSS number: 4 I IWs '4 o Stock preparation: t00 g NaCI/I (dissolve 50 g NaCI in 500 ml deionized water) Dilution prep (m ) 600 800 1000 1200- 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume mL) 1491 1488 1485 1482 1479 _Total volume (mL) 1500 1500 1500 1500 1500 Test organismsource information: Test information: Organism age: I< 24-hours old Randomizing template color: be.ANG.. Date and times organisms were born OS-05-OGI OA.1ks T"O&,6 Incubator number and shelf between: location: Culture board: CA'-U - Replicate number: 1 2 3 4 1 S 6 7 1 9 10 YWT batch: Culture board cu number: -L I 10 o I It. 1 121 12.W Transfer bowl information: pH = 1. 4 SU Temperature = -LS. .7. 'C Selenastrum batch: - Daily renewalinformation: Day Date Test initiation and feeding, MNHSW Analyst renewal and feeding, or batch used termination, time 0 OS-o04- oA oiok o4-2 T i'-o* 1 OS'0-00%c 001
.0o OAk- -R-C?& A 3 _ _ _ __ OsO 01__
4001-0 05 oso- ________ A 6 C5o-I1- CA 0-a n 6L1 9-Control information: Acceptance criteria Summary of test endpoints:
% of Male Adults: )7 <20% 7-day LC 50 ) 4c-Do
% Adults having 3 d Broods: 33-& 1 001, > 80% NOEC 00-
%Mortality: <20%
5 LOEC 10000 SMean Offsorina./Fernale: ,:*. G _>f 5.0 offspring/female Ch V Mean OsC3.
% CV: 4-. 5 -o < 4U.U '/.
U IC 2-- s i Io16t. 2-Iage 98 of 107
Page 2 of 6 ETS Env,. Mm ~ Species: Ceriodaphniadubia CdNaCLCR #: VA_ CONTROL Survival and Reproduction Data Replicate number Day 2 3 4 5 6 7 8 ,9 10 I Young produced 0 0 0_ C) 0 0___ 0 C) Adult mortality __ __j _.- t .__- \I- _. t_. 2 Young produced D CI c__l C__ Adult mortality JL.4 -- k. ,-- ' '- L._1,,-t 3 oung produced Adult mortality I t__ 0-\,- (L 7 C 0 0 0
%k 4 Y'oung produced IPk 5 Young produced i- '. \L__ tk- "L %
"*. ,A_* I 11.5'*
Adult mortality 'I .- \- \.- \ .- 6 Young produced !,... ' [* .[ . L" A Adult mortality 7 jYoung produced j ",j I -'j _ '2. . l j 2 I ____ Total young produced lo I olD3li.A 01 ,]OIV 3 1l. Final Adult M ortality _* _... __... ... -. _.. . . X for 3rd Broods X - C- " - . . vote: Adaul mortality (L = live, U aeaoj, 313 = spur brood (single oroou spiu oetween two uays), CU = carry over korispring carried over with adult during transfer). Concentration:
% Mortality:
Mean Offspring/Female: 33. 6
" 600 mg NaCI/L Survival and Reproduction Data Replicate number
.Day 1 2 3 4 5 "6 7 8 ' 9 10 I Young produced o 5 3 - c" I~
I Adult mortality L- L. __ ____ k,_.__ 2_ Yon rducedr~l~ JCj) Qj ~C) CI)IL IAdult mortality L 1 I U_+ I 3 Yon prdue 1i1 - I I ______ .__ U _ L_ ._ - _ Adult mortality .. __ _ _ .. ._ 4 Young produced I S __ k. Adult mortality \.- __t'_~ -I~-I I'-_~~V~I 5 6 Young produced Young produced I' Z lC) C)j In--i--- 'A "l 0I ~__ . __Adult mortality \ - \~_-'-I - ~IU U 7 Young produced 0 C) %!S 0 l& IS 0 L (a I (I Total young produced .3q T5 " ý3-1 4 3 J 3S J,4 Final Adult Mortality - . \_-- \ . _ . _.. ._ ' " __
,vore
.... A..Il I lultali* (L - lvU-I1.),_ .Auu
- _.
-S~i nruA t siiug _
uc ruui. i _:[ r.A ei
..... two Udy), fLu *-. ... ....over l k0^_.;l corted carried ov*r over woiality with a adultt during L live, r anae),
D transfer). B - sprt brood ksingle ood spit between o days), U ý carry over kOisprlng
. ~
I % Mortality: 1 (V. 1 i Mean Offspring/Female: 3"t.& I I % Reduction from Control: I- 3.61 [age 99 of 107
r Page 3 of 6 TS Specie s: Ceriodaphniadubia CdNaCLCR #: ___ Ii 800 m NaCI/L Day 1..... j 2 3 Survival andReproduction Data 4
. Replicate number 5 6 7 8 9 10 I Aogrdultmraled
- J- L__ [_. L_ L_
°1J 11 2 Young produced Adult mortality 0 ]J 0 C 1 =)
c I I Adult mortality Young produced I IL %,- I __. C-0 c
- k. -
.sic 'c
% k.-_ _._
)
I 4 Adult mortality Young produced O k--_ 1 _ i0 1 1 0 C I~T 01~ I V Id .. Adult mortality L[_ - -I._ - '.. I 7[ Young produced[ Adult mortality kI
--. I i s i0 .Ž 7 Young produced- - 16: 1t, . IS- I&'[*
I C t*S 0* 0 Total young produced .3.3 3313$ I .n 32 3' 33 Final Adult Mortality .. . . . j._.._ k- U 1,. Note: Adult mortality (L live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult duringw transfer). Conceniration:
% Mortality:' 07.
Mean Offspring/Female: 2Z&. L
% Reduction from Control: I I
. 7.7.
1 1000 mg NaCI/L Survival andReproductionData Replicate number Day _2_1_3_1_ _5f 1 2 4 5 663 8 9 t10 I Young produced n______ C) _ (0 0_ Adult mortality ._ , .. I... k. I __. k J_. _ 2 Young produced I '- .K)I )' +-II C) 1 _ 0_ [ Adult mortality I L-[ -- - - l
- I *- 1*-
5_ Young Young Adult produced produced Adult mortality, mortality 1~-_ 1k{~~ L Iki- ._4[ % IIU-f1~ LI [ L1 T
.. 1~ ý.. k _... I.
6 Adult mortalit Young produced D_clT0 1J 0- I) C IS __ Adult mortality [L _L U_ =L_~ U J_-- \_ '--_ 7 Young produced L, Iz IS
- l. * " o- '94) I()3 Total young produced [ 2*'* 30 30 21 3o *0o 3 -
Final Adult Mortality U v T. ' . .__ 7..-....=. cried tUArw I ;I~tI~ =LI:VL -U ) .I. J ~iIUUU UUU~L1UL~ILUUy) A WYU~~IL)i~ carried oover with adult duL. oadulty with I tr during afe transfer). ), brood e_brood_ be__.-- __t __i_ _ over _ _twoys), C0U-- arry _ _piIg [Concentration! 4. I % Mortality: p I oD -7 I I Mean Offspring/Female: 3o.1 I
% Reduction from Control: 8.67. 1 tage 100 of 107
Page 4 of 6 I Ern..MT.a.. Species: Ceriodaphniadubia " CdNaCLCR #: g81
-1200 mz NaCI/L Survival and Reproduction Data Replicate number Day __ 1 2 3 4 5 6 7 8 '9 10 I [ Young produced : C )* a)
- 0 C) C) r) C Adult mortality . . - ...
2 Young produced ©l 0* 00 0 Q1 C) 0 0 0) IAdult mortality I +,-I I- --I +I*1T 3 Youngproduedl ý ý 0 1 QA c nI I O
" Adult mortality L ,,-1 I - * [ v- j
- L.
Adult mortality _ Young produced -I I _ 065,eAL-4JQ+/-.
.A- CAILICN O'eAL Adult mortality "7 Young produced .- OC)',* * * * *s Total young produced Is 8 I g i -t l51 Final Adult Mortality L k- 1. _. _ I_._ _ ,
Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO carry over (offspring carried over with adult during transfer). Concentration:
% Mortality: 07 Mean Offspring/Female: I5-4.
% Reduction from Control: -52,'/77 I 1400 mg NaCI/L Survival and Reproduction Data
_Replicate number Day 1 2 3 4 5 6 7 8 9 10 I I Young produced Adult mortality _ ._
) ) () o C ) 0
- 2. Yung produced ILQ c___ C___ C___ C___ 1___
6 Ad'2ult mortality I 0] Young produced]dC)tm)ral1 Adult mortality [L1
.]__UJLJ1 L-i 0_1
= 0 0 01 I_'V&_1 1 1 Young produced [ 2 L1 (2)"C. ',
5i Young produced )kC L *I~eA'b W-4" 6 Young produced C) 4 0 Q 0 01 Adult mortality. I -,-- \I .- - I - Total young produced " !
-5 l I 5 3 q Final Adult Mortality [ - * ' - - '- 1 '- '- '_
Note- Adult mortality (L = live, U = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer). I Conc. l /. lkýrtalitv' I ip*A'Ii Mean Offspring/Female: ' -q I
% Reduction from Control: -7%.0.
rage 101 of 107
W UI En, EnJ Verification of Ceriodaphnia Reproduction Totals Iron nental Testing Solutions, Inc. C on rol 1000 mg NaCIIL Dy . Replicate number Total Day Reicate number - Total 1 2 3 4 5 6 7 8 .9 10 1 2 3 4 5 6 7 8 9 10 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 .0.0.0 .0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 .0 0 0 '0 0 0 0 0 0 4 6 4 5 5 6 5 5 6 4 4 50 4 6 4 6 5 4 5 5 5 4 5 49 5 13 1 13 12 1 12 1 12 1 14 12' 12 13 13 126 5 13 1 12 10 10 13 11 1112 12 115 6 0 17 0 :0 0 0 16 0 17 15 65 6 0 0 0 0 0 17 0 0 0 15 32 7 17 01 17 14 16 16 _ 0 15 00 95 7 13 14 12 15 13 0 14 14 16 0 111 Tat 36 34 34 31 34 I 35 33 33 34 32 336 Total 32 29 30 30 27 35 30 30 32 32 307 6, 0 g NaC1IL 1200 mg NaCnL Da Re plicate number Total Re plicate number Total 1 2 3 4 5 6 7 8 9 Tt 1 2 3 4 5 6 7 9 10 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 o0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 _ 0 0 0 0 0 0 0 0 0 4[645 5 5 6 5665 53 4 5 5 5 4 4 5 4 4 4 4 44 S 12 113 14 14 14 13 13 1 1 13 13 130 5 5 5 7 3 7 5 .5 6 5 5 53 6 1 0 18 0 16 0 0 180 0 0 52 6 0 7-0 0 0 0 0 0 1 0 6 7 17 0 0 18 15 016 1616 113 7 5 9 6 5 8 6 4 7 5 0 55 To 5 35 34 35 37 34 36 33 35 34 348 Total 15 19 18 12 19 16 13 18 14 15 159 8N ng NaClI/L .1400 mg.NaCML Re plicate number Total Day Replicate number Total 1 2 3 4 .5 6 7 8 9 1 Tt 1 2 3 4 5 6 7 8 9 10 1 0 0 0 .0 0 0 0 0 ,0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 .0 0 0 0 00 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 01 3 0 0 0 0 0 0 0 0 0 0 0 5 4 4 5 5 5 6 5 5 5 49 4 5 3 4 2 5 4 3 3 3 2 34
- 5. 13 13 I1 13 I1 12 13 .12 12 12 122 5 4 0 3 3- 5 5 6 0 0 4 30..
0 0o 00 17 0* 16 49 6 0 4 0 0 0 0 0 2 0 0 .6 7 16 1561 15 1 15 00 112 7 3 1 0 0 5 2 0 0 0 3 14 To I 34 33 I 30 33 33 35 36 32 33 33 332 Total 12 8 7 5 15 11 9 5 3 9 84
*- - - a a a a
r-4 0 U Ceriodaphniadubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 E._ z Quality Control Iro rmental Testing Solutions, Inc. Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #89 Test dates: May 05-12, 2009 Reveiwed by: Concent ation Replicate number Survival Average reproduction Coeffcient of Percent reduction from (mg/L aCI) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) variation (%) control (%) Cont ol- 36 34 34 31 34 35 33 33 34 32 100 33.6 4.3 Not applicable 60( 35 35 34 35 37 34 36 33 35 34 100 34.8 3.3 -3.6 80( 34 33 30 33 33 35 36 32 33 33 100 33.2 4.9 1.2 100 *32 .29 30 30 27 35 30 30 32 32 100 30.7 7.0 8.6 120 15 19 18 12 19 .16 13 18 14 15 100 15.9 15.8 52.7 140E 12 8 7 5 15 11 9 5 3 9 90 8.4 43.2 75.0 Dunnett's I4SD11 value: 2.291 MSD = Minimum Significant Difference PMSD: 6.8 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10"' percentile) = 13%. Upper PMSD bound determined by USEPA (90"' percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 2,01a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmen al Protection Agency, Cincinnati, OH.
0 0 0 0 Statistical Analyses -.4 F nvlronmentalTesdng Solutions, Inc. Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/5/2009 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 5/12/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: - Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mg/L 1 2 3 4 5 6 7 8 9 10 D-Control 36.000 34.000 34.000 31.000 34.000 35.000 33.000 33.000 34.000 32.000 600 35.000 35.000 34.000 35.000 37.000 34.000 36.000 33.000 35.000 34.000 800 34.000 .33.000 30.000 33.000 33.000 35.000 36.000 32.000 33.000 33.000 1000 32.000 29.000 30.000 30.000 27.000 35.000 30.000 30.000 32.000 32.000 1200 15.000 19.000 18.000 12.000 19.000 16.000 13.000 18.000 14.000 15.000 1400 12.000 8.000 7.000 5.000 15.000 11.000 9.000 5.000 3.000 9.000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 33.600 1.0000 33.600 31.000 36.000 4.255 10 34.200 1.0000 600 34.800 1.0357 34.800 33.000 37.000 3.262 10 129.50 75.00 34.200 1.0000 800 33.200 0.9881 33.200 30.000 36.000 4.877 10 95.50 75.00 33.200 0.9708
*1000 30.700 0.9137 30.700 27.000 35.000 7.045 10 68.00 75.00 30.700 0.8977
*1200 15.900 0.4732 15.900 12.000 19.000 15.814 10 55.00 75.00 15.900 0.4649
*1400 8.400 0.2500 8.400 3.000 15.000 43.179 10 55.00 75.00 8.400 0.2456 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.81542361 1.035 0.21736896 0.95569016 Bartlett's Test indicates unequal variances (p = 8.87E-03) 15.3749275 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 800 1000 894.427191 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point mg/L SD 95% CL Skew IC08*8 2Stt 42 6664f'107 76408s7'*629 Olt 19821A Al 44 IC10 993.6 29.0004806 921.551731 1015.99639 " -0.8830 IC 15 1022.02703 8.689644 1004.45361 1037.76034 -0.3344, IC20 1045.13514 7.64509171 1029.66986- 1060.12003 -0.0590 IC25 1068.24324 .7.05805813 1055.32988 1081.38741 0.0234 1C40 1137.56757 7.32664661 1123.52221 1151.44087 0.2385 IC50 1183.78378 9.03530003 1167.78076 1201.21731 0.2331 05-05-09
S '9 Statistical Analyses S Environmental Testing Solutions, Inc. Used for PMSD calculation only. Ceriodaphnia Survival and Reproduction Test-Reproduction Stant Date: 5/5/2009 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant Enc Date: 5/12/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sar ple Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Co ments: Cenc-mg/L I 2 .3 4 5 6 7 8 9 10
-Control 36.000 34.000 34.000 3 1.000 4.000 35.000 33.000 33.000 34.000 32.000 600 35.000 35.000 34.000 35.000 37.000 34.000 36.000 33.000 35.000 34.000 800 34.000 33.000 30.000 33.000 33.000 35.000 36.000 32.000 33.000 33.000 1000 32.000 29.000 30.000 30.000 27.000 35.000 30.000 30.000 32.000 32.000 1200 15.000 19.000 18.000 12.000 19.000 16.000 13.000 18.000 14.000 15.000 1400 12.000 8.000 7.000 5.000 15.000 11.000 9.000 5.000 3.000 9.000 Transform: Untransformed 1-Tailed C nc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
-Control 33,600 1.0000 33.600 31.000 36.000 4.255 10 600 34.800 1.0357 34.800 33.000 37.000 3.262 10 4.198 2.287 2.291 800 33.200 0.9881 33.200 30.000 36.000 4.877 10 0.399 2.287 2.291
*1000 30.700 0.9137 30.700 27.000 35.000 7.045 10 2.895 2.287 2.291
*1200 15,900 0.4732 .15.900 12.000 19.000 15.814 10 17.667 2.287 2.291
*1400 8.400 0.2500 8.400 3.000 15.000 43.179 10 25.153 2.287 2.291 Au iliary Tests Statistic Critical Skew Kurt Kol ogorov D Test indicates normal distribution (p > 0.01) 0.81542361 1.035 0.21736896 0.95569016 Ba lett's Test indicates unequal variances (p = 8.87E-03) 15.3749275 15.0862722 Hy othesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dujnett's Test 800 1000 894.427191 2.29089729 0.06818147 1241.68 5.01851852 6.4E-36 5,54 Treatments vs D-Control 05-05-09
- Page 5/ of 6 I
Species: Ceriodaphniadubia CdNaCLCR#: " Daily Chemistry: Day
- 0. 1 2 -
S .Analyst f Lht-, PtAL%,- Concentration Parameter I SpH (S.U.) T"H 7,** --* r* CONTROL DO( m1L) Conductivity (inmhos/cm) 3 // q ' . Alkalinity (mg CaCO3/L) 6 7- otq Hardness (mg CaCO 3/L) Tem perature -LA 1_ IL pH (S.U.) __.,___ "-_ '.55____ DO (mg/L) 1'7 7A 600 mg NaCI/L Conductivity (ýIhos/cm) Temperature (0c )j_ _ .o D .A 14 . _ _ - J4. 1c ', S. PH (S.U .) t ,a , -.7 ,5 "72 T 7.5S ,S 0 . r. " S -A DO (img/L) - , - -. -- 800 mg NaCI/L Conductivity (iimhos/cm) Temperature I 2L (0c) 0."__ .______ _ q_..__ 2_ . ______. pH (S.U.) DO (rag/L)
"-4,q 7 *]'3 :57-1l - 4*
*1-2 7q 1.5L1
- q
-4
-4 1000 mg NaCVL Conductivity (piihos/cm) 0 Temperature (0c) ZI.9 q. qq 7* c z _~9 t~
pH (S.U.) _ - _6 . - - 4. g-._
"7 " __.___
DO (rm/) "-. "- 1200 mgNaCImL Conductivity - 5l (pmhos/cm)p Temperature 2. ~9 2. L. pH (S.U.) H116 i31k -315 *1~ TO 7.578 11m. DO (mg/L) 1400 mg NaCI/L Conductivity (mhos/cm) Temperature (0C STOCK I Conductivity I (4mhos/cm)
.Initial Final Initial Final Initial Final age 106 of 107
IlETS Page 6 of 6 Species: Ceriodaphniadubia CdNaCLCR #: c* Day6 3 4 56 __ __ __ __Analyst tALt" LA-6 I LN ~Arr Concentration Parameter IpH (S.U.) .13 1 t 7.O DO (mg/L) iO T1~73o .~ I~- CONTROL Conductivity 32- z (ýtmhos/cm) Alkalinity (mg CaCO3/L)' 61 Hardness (mg CaCO 3/L) ( Temperature 7AS -.. l.S... " .4 (0c) p (S.U.) 3 ".7, .-. DO 1./L 8.0 1159.0.1 600 mg NaCVL Conductivity*q ]* ([mhos/cm) Temperature .VA.*-I 0C) pH( . . -_.- _,__ - - - 800 mg NaCI/L Conductivity BI 0 ( )Temperature " - ".*O "t-,.s "L .' .\ "q.* "q L 0 pH (S.U.) *%.0 1.50 1.47 'tA* */ 1000mgNaCI/L Conductivity I l/l ~ *t l (pmhos/cm) Temperature DO ILA 4,1-93 73/L "t4* " 12000 mg NaCL/L (°C) PH Conductivity 1-60 .50 1. *S.O 0 L (pmhos/cm) Temperature Is.s~ri. VA t'-i RS __________ _ ( pH___ S.C) _______1_ ________ - -I - DH (S.U.) 63 17;:; 7.,l . IIIq,'fl -4,2 T"1 q4"I, 1 I DO (mz/L)
*1400 mg NaCIL. Conductivity
(*Imhos/cm) I
-2B-3. - 5 ., 1?. ""
Temperature (0c)
- 0-
'. 3. OCK - -fn----------
- mho c Initial 11 Final I Initial. Final - Initial I Initial Final e 107 of 107
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 - - - j~iNTEROFFICE SB-2A-S QN) TN0026450 103 G F-FINAL SODDY-DAISY . -TN37384 PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MrlNIT0PRINF PFR11io I EFFLUENT FromI1From 09 105 YEA 1011 .T To 1o91o5 MO DA 31ý *** NO DISCHARGE ATTN: Stephanie.A. Howard NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH SAMPLE 7.6 ........ 8.7 12 0 20/31 GRAB MEASUREMENT ý00400 1 0 0 , PERMIT'~ ~ ~ ~ *,- *~ r : SU ~ THREE,< GRPAB !EFFLUENT GROSS VALUE REQUIREMENT : t; :: :;.-. MI'MiNIM UM t*:<;,:*** ......... MAXIMUM WEE 831K 4-L
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<84 <122 26 < 19 0 1 GAB 00556 1 0 0 ~ 2PERMITI 1~90 25LBS/DY F1542 MG/L IEKY GRAB EFFLUENT GRSSVLUIREMO r IM2VG
,EFFLUENT GROSS VALUE M. AG . DAILY MX MO AVG *DAILY MX FLOW, IN CONDUIT OR THRU SAMPLE 1.251 2.488 03 ... 0 31 / 31 TOTALZ TREATMENT PLANT. MEASUREMENT
- 1. 0 0 PERMIT MGD .50050 SEE TOTALZ REPORT REPORT EFFLUENT GROSS VALUE REAUIREMENT E T MA'MO AV SAMPLE MEASUREMENT PE~RMIT << r~~i SAMPLE MEASUREMENT SAMPLE MEASUREMENT PERMIT -'h REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel T E E Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engfneer information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 09 06 09 Sequoyah Site.Vice President accurate, and complete. I am aware that there are significant penalties for submitting false . SIGNATURE OF PRINCIPAL EXECUTIVE information, includingthe possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
The Turbine Building Sump (TBS) was discharged directly to the Yard Pond (YP) on 5/5/09 @ 0850 - 5/9/09 @ 1425 and on 5/11/09 @ 1625 - 5/11/09 @ 1740. EPA Form 3320-1 (REV 3/99) - Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved, .Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Address P.0. BOX 2000
... -. JINTEROFFICE SB-2A-SQNL- TN0026450 167 F-FINAL F
SODDY - DAISY TN 37384 PERMIT NUMBER ýDSCARGE NUBR METAL CLEANING WASTE POND Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ! . flAM ITn DlIKlt Ivl*./ill I *'J*II*1%.7 DC:DInMr
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II EFFLUENT YEAR I MO DAY I MO DA NO DISCHARGE @ ... ATTN: Stephanie A. Howard From 0 09 1 05 01 1 To 1 09 1 05 1 31 I NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE MEASUREMENT -* 12 100400 1 0 0 PERMITh , ***~~~~****<~~.0; 6 _ 9. SU DA ILYk. .GRAB9 IEFFLUENT GROSS VALUE REQUIREMENT 0 1 -.- . I~4.
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ýEFFLUENT GROSS VALUE DIYX. I.~
IRON, TOTAL (AS FE) SAMPLE 19 MEASUREMENT 19 01045 1 0 0 1 PERMIT 'I~****'~~~~****- * -- ~ -. 10 , MGIL .. , DAILY. COMPOS EFFLUENT GROSS VALUEREUEMT & 'ALy X FLOW, IN CONDUIT OR THRU SAMPLE * ******** ITREATMENT PLANT MEASUREMENT 03 ** 50050 1 0 0 FERMIT . ... MNIG. .. . DI Y c-LCI R:8EQUIREMýN EFFILUENT GROSS VALUE.E RUIREMNT MO AVGý - DAILY MX NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE
.direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted, Based on my inquiry of the person or persons who manage the system, or those persons directly rebponsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 09 06 09 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3199) . Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FacilitvName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Address P.O. BOX 2000 - - - A1INTEROFFICE SB-2A-SON. . TN0026450 110 G F-FINAL SODDY - DAISY TN 37384 PERMIT NUMBER 6DISCHARGE NUMBER] RECYCLED COOLING WATER Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD 7 EFFLUENT I R DAY .. NO DISCHARGE XX ATTN: Stephanie A. Howard From L09 I 001 To l0i I INOTE I I. reau I I IUn.stIUIIo UeoIUe comUpIeInJIgLII tsfo rm. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS I I I I TEMPERATURE, WATER DEG. SAMPLE !CENTIGRADE MEASUREMENT 04 04 00010 z 0 0 1PER MIT DEG C DEG C IJýRy 01-PAR-4 REQUIREMrENT~ 38.3 INSTREAM MONITORING DAILY MX PH SAMPLE ,IrW MEASUREMENT 12 00400 1 0 *0 - PERMiT ~ ** *w;~ 6.0 M I9.0 M SU WIEEKLY GRAB EFFLUENT GROSS VALUE 'REQUIRHEMENýT ~ D I *~'~'MAXIMUM SOLIDS, TOTAL SUSPENDEID SAMPLE * * **** ** MEASUREMENT 19 00530 1 0 0 PERMIT -;'>r REQUIREIMENT 30 MGIL 1DAIL-Y- ýCOMPOS EFFLUENT GROSS VALUE flAILY MX OIL AND GREASE SAMPLE ******** ********
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- 1 0 0 -RE RM17 REQUIREMENT 0.10. MG/L* WEE77KLY GRAB-4 EFFLUENT GROSS VALUE DAILY MVX SAMPLE MEASUREMENT PERMIT - 4 REQUIREMENT- ~ ~J-NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my I --
direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the-system, or those persons directly responsible for gathering the Principal Enviro nmental Engineer Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PR
!information, including the possibility of fine and imprisonment for knowing violations.
INCIPAL EXECUTIVE OFFICER OR AU THORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period Page 1 of 1 3320-1 IREV3/991 Form 3320-1 EPA Form EPA (REv'3/99) editions may Previous editions Previous may bebe used used Page 1 of 1
PERMITTEE*NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name . TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)' (SUBR 01 ) OMB No. 2040-0004 Address P.Oi BOX 2000
- INTEROFFICE SB-2A-SQN. TN0026450 L 110 T F-FINAL F
SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facilitv TVA - SEQUOYAH NUCLEAR PLANT* Location HAMILTON COUNTY RAN llITnlPIf'NC PF: RIfn I EFFLUENT YEAR MO DY YEAR MO DAY N ATTN: Stephanie A. Howard From l 1 1 *** NO DISCHARGE
- NOTE. Read instructions belore completing this form.
rI ...... SML -FEUnIY PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FRQUENCY1 SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS I I- I H H t r T IC25 STATRE 7DAY CHR SAMPLE ý,CERIODAPHNIA MEASUREMENT 23 TRP3B 1 0 0 PERMIT REQUIREMENT
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PERMIT REQUIREMENT _ I_ -. , 4- __ __ __ -4 _ - - ___ __ . H--- -- r. .. .. SAMPLE MEASUREMENT PEIRMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my . - direction or supervision in accordance with a system designed to assure'that qualified personnel
*Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVI TYPED____PRINTED_ information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Address P.O. BOX 2000 ANTEROFFICE
.- SB-2A-SQ .N. . TN0026450 G ] F-FINAL F-116 SODDY - DAISY TN 37384 PERMIT NUMBER ýDISCHARGE"NMBER BACKWASH Facilit* TVA - SEQUOYAH NUCLEAR PLANT i " -k Location HAMILTON COUNTY MONITORING, PFRII)D i EFFLUENT I YEAR 7
MO I DAY YEAR MO DAY NO DISCHARGE ATTN: Stephanie A. Howard From 09 05 01 T 0 05 31O NOTE: Read instructions before completing this form. der penalty, of law that his docurt ant and all attachments were prepared under my I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER der penalty of law that this documnent and all attachments were prepared under my F Timthy . Clarydirect Timohy
. Claryproperly ion or rsupervision in accordance with a system designed to assure that qualified personnel ga ather and evaluate the information submitted. Based on my inquiry of the person or persons wh0omanage the system, or those persons directly responsible for gathering the information the information submitted is, to the best of my knowledge and belief, true, Principal Environmental Engineer Sequoyah'Site Vice President accurate, and complele. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information , including the possibility of fine and imprisonment for knowing violations.
TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT L ________________________________________________ L ____________________________ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. E~M rurrri 3JZU-I fr¶~V or~O~ f-re vtous eoirrons may oe useo Page 1 of 1 EPA Form 3320-1 (REV 3/99) P~reviouseditions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) M " OMB No. 2040-0004 (SUBR 01) Address PO. BOX 2000 INTEROFFICE
.- SB-2A-SON) .. .. .. TN°026450 117 G F-FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER BACKWASH Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT YEAR MO DAY YEAR MO DAY NO DISCHARGE = ...
ATTN: Stephanie A. Howard From 09 105 101 TO 09 105 131 NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF. TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE ** 0 9A 0 1 / 31 VISUAL MEASUREMENT 01345 1 0 10 ~ ~PERMIT >, .**4~ <REfPORT, PASS=0 $EE VISUAL EFFLUENT GROSS VALUE *EiQ ' h. FAIL=1 PERMIT __ __ _ _ _I_ ___ MO TOTAL OIL AND GREASE VISUAL SAMPLE 0 94 0 1/31 VISUAL MEASUREMENT 0"4 **
ý84066 1 0 0 PEMT REOT YS1~~-W SEE >VISUAL REUIREMENT'I-EFFLUENT GROSS VALUE , REQ.MO TOTAL NPERMIT=O PRjf SAMPLE MEASUREMENT PERMIT j..
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REQUIREMENT' SAMPLE MEASUREMENT SAMPLE MEASUREMENT PERMIT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cenify under penalty of law that this document and all attachments were prepared under my -, I l - TELEPHONE DATE
.direction or supervision in accordance with a system designed to assure that qualified personnel 'i
- J. j "" -6 Timothy P. Cleary properly gather and evaluate the information submitted Based on myinquiry of the person or persons who manage the system, or those persons directly responsible for gathering the . Principa Environmenta Engineer information, the information submitted is, 1o the best of my knowledge and belief, true, 423_843-6700_09 06 09 Sequoyah Site Vice President accurate, and compele. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE
-__information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED i . CODE
.COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previouseditions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) 0MB No. 2040-0004 (SUBR 01) Address P.O. BOX 2000
- .-INTEROFFICE SB-2A-SQN. . TN0026450 118 G F -FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER] WASTEWATER & STORM WATER Facilit TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD I EFFLUENT YEARI MO I DAY YEARI MO 1IDAY NO DISCHARGE XX ATTN: Stephanie A. Howard From 09 05 01 To 09 1 05 T31 NOTES Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE. , 19 MEASUREMENT 19 00300 1 0 D PERMIT :-MI***- - - i**-****.. ,* - MGIL M.G-:L _ 'TWICE/ GRAB: EFFLUENT GROSS VALUE
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SOLIDS, TOTAL SUSPENDED SAMPLE ** **9 MEASUREMENT 100530 1 0 0 1 PERMIT **" .* .MG/L TW/NICE/I GRAB EFFLUENT GROSS VALUE - DAILY MXMENT WEEK< iSOLIDS, SETTLEABLE SAMPLE **** *25 MEASUREMENT 25 00545 1 0 0ý PERMIT>K " *** ~ ~ >>?****~- 1.0'- MUL - ONCE/ >>GRAB~ EFFLUENT GROSS VALUE R M>>DAILY MXV'K 'K' MONTH FLOW, IN CONDUIT OR THRU SAMPLE ** TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 RPERMIT ET REPoT MGD ONCE/ ESTIMA SAMPLE MEASUREMENT REQUIREMENT I SAMPLE MEASUREMENT REQUIREMENT ->'KKP SAMPLE MEASUREMENT
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I NAME/TITLE PRINCIPAL EXECUTI\ fE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE I direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, Principal Environmental Engineer 423 843-6700 09 06 009 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information. including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR{ MO DAY I TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS. (Reference all attachments here) . During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of I}}