ML13289A205

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Sequoyah Nuclear Plant - Annual Water Withdrawal Updates for 2008
ML13289A205
Person / Time
Site: Sequoyah  Tennessee Valley Authority icon.png
Issue date: 08/14/2008
From: Howard S A
Tennessee Valley Authority
To:
Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
Shared Package
ML13289A109 List: ... further results
References
Download: ML13289A205 (187)


Text

{{#Wiki_filter:S58 080814 800-NPDES CORRESPONDENCE August14,2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JULY 2008 Enclosed is the July 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please coritact me at (423) 843-6700 if you have any questions or comments. Sincerely, ,j 1 d Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, BR 4T-C A. A. Ray, WT 7C-K J. D. Smith, OPS 4A-SQN G. R. Signer, WT 6A-K EDMS, WT CA-K (Enclosure) DMR0807.doc Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 August 14, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JULY 2008 Enclosed is the July 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, . )-hoL<1A'--"- 0 JJotUc&c d Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Pnnted on recycled paper PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ ______ _ Facility_ .JYA -_§f:QUOYAH NUCLEAR PLANT _____ _ Locatio_!L __tjAMIL TOli_COUNTY_ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMRI TN0026450 PERMIT NUMBER From 101 G DISCHARGE NUMBER DAY 31 MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE [J *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION I NO. I FREQUENCY/ SAMPLE EX OF TYPE WATERDEG. 0 WATERDEG. 0 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my .. -td TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 08 08 14 information, the information submitted is, to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE L__ ---------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2 DMR Attachment CCW Data

  • CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 07/09/2008

@ 1200 0.24 mg/1 07/11/2008 @ 1033 CLS TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 07/09/2008 @ 1150 <0. 10 mg/1 07/11/2008 @ 1044 CLS TN EPA 8015 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRI MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Add reS§_ 2000 ___________ _ ______ _ Facility_ JYA -.§EQUOYAH NUCLEAR PLANT _____ _ Locatio.n_ _!:!AMIL TOl:!..COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER

CHLORINE, TOTAL RESIDUAL 50060 0 0 EFFLUENT GROSS VALUE MPERATURE
  • C, RATE OF CHANGE 82234 1 0 0 FFLUENT GROSS VALUE TN0026450 YEAR From I os I --I -. I QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM ********

101 G DAY 31 DIFFUSER DISCHARGE EFFLUENT

      • NO DISCHARGE D *** NOTE: Read instructions before completin!l this form. QUALITY OR CONCENTRATION I NO. I FREQUENCY EX OF AVERAGE MAXIMUM UNITS ANALYSIS 0.019 0.038 19 0 29/31 SAMPLE TYPE GRAB NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my

.. TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 08 08 14 information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY TYPED OR PRINTED CODE ----COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured:

1. Biodetergent 73551 (max. calc. cone. was 0.018mg/L--Iimit 2.0mg/L)
2. MSW-101 (max. calc. cone. was 0.062mg/L-Iimit 0.2mg/L)
3. H-150M (max. calc. cone. was 0.038mg/L--Iimit 0.050mg/L)
4. H-150M (low detection level analytical method was <0.020mg/L--Iimit 0.050mg/L)

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2 I ' PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR} MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Addres_L 20QQ_ ___________ _ -__ _§.ODDY-DAISY _ _IN 37381._ ______ _ Facility _ _IYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!]_ .JjAMIL TOli.COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER 0 0 EFFLUENT GROSS VALUE TN0026450 YEAR From I os I -. I -* I QUANTITY OR LOADING AVERAGE MAXIMUM UNITS ********

101 T BIOMONITORING FOR OUTFALL 101 EFFLUENT 31 NO DISCHARGE D ... DAY NOTE: Read instructions before completinQ this form. QUALITY OR CONCENTRATION I NO. I FREQUENCY' SAMPLE EX OF TYPE MINIMUM AVERAGE MAXIMUM ********

NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary property gather and evaluate the information submitted. Based on my inquiry of the person or ' persons who manage lhe system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in July 2008. EPA Form 3320-1 (REV 3/99) Previous editions may be used Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 843-6700 08 08 14 I AREAl NUMBER YEAR MO DAY CODE --------Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Add res§_ ...f.Q.,_BOX 2000 ___________ _ TN0026450 ______ _ Facilitv_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l_ _!:::!AMIL TOJ::!.COUNTY __________ _ ATIN: Stephanie A. Howard PARAMETER 0 0 TOTAL SUSPENDED 0 0 0 0 GROSS VALUE GROSS VALUE YEAR From I 08 I -. I -* I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320*1 (REV 3/99) Previous editions may be used 103 G LOW VOL. WASTE TREATMENT POND EFFLUENT 31 *** NO DISCHARGE D ... DAY NOTE: Read instructions before completinQ this form. QUALITY OR CONCENTRATION I NO. I FREQUENCY I SAMPLE EX OF TYPE ANALYSIS d TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 08 14 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ ___ DAISY _ ____TN 3738i._ ______ _ Facilitv_.JYA-_§E:QUOYAH NUCLEAR PLANT _____ _ Locatio..!l_ _!:!AMIL TO_!i_COUNTY_ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMRJ From TN0026450 PERMIT NUMBER YEAR 08 107 G DISCHARGE NUMBER MAJOR (SUBR 01) F-FINAL METAL CLEANING WASTE POND EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 ATTN: Stephanie A Howard NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM AVERAGE PH ********

0 0 50050 1 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used ******** c Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE DATE 423 I 843-6700 08 08 AREA I NUMBER YEAR MO CODE _L_ Page 1 of 1 ! 14 DAY PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres.L_ __E.Q,_BOX_1900 ___________ _ ______ _ Locatio.!l._ .J::!AMIL TOl:!_COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMR) TN0026450 110 G From I os I _. I --I MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMS No. 2040-0004 NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION I NO. I FREQUENCY/ SAMPLE EX OF TYPE QUANTITY OR LOADING 0 0 I;)ULiu;:., TOTAL SUSPENDED 0 0 TOTAL RESIDUAL 1 0 0 EFFLUENT GROSS VALUE AVERAGE MAXIMUM ********

UNITS MINIMUM E MAXIMUM ********

04 NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing Violations. TYPED OR PRINTED COMMENTS AND OF ANY (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT UNITS 04 TELEPHONE DATE 423 843-6700 08 08 14 I AREA I NUMBER MO DAY CODE Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Add res.§_ 2000 ___________ _ TN0026450 -__ _.§.ODDY-DAISY _ _.IN 3738£.. ______ _ Facility_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l._ .J::!AMIL TO_!LCOUNTY __________ _ ATTN: Stephanie A. Howard From 1 __ I _. _

  • PARAMETER 7DAY CHR 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** NOTE: Read instructions before completinq this form. QUALITY OR CONCENTRATION I NO. I FREQUENCY/ SAMPLE EX OF TYPE vu"--c( TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 08 14 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res.§_ ___________ _ -__ _§.ODDY-DAISY _ __.IN 37381._ ______ _ Facility_ ...JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l_ .J:!AMIL TO.Ji.COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 116 G YEAR DAY From I oa I --I --I 31 MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT NO DISCHARGE D *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completing this form. QUALITY OR CONCENTRATION I NO. I FREQUENCY I SAMPLE EX OF TYPE PARAMETER QUANTITY OR LOADING , FLOATING (SEVERITY) AND GREASE VISUAL 0 0 EFFLUENT GROSS VALUE AVERAGE MAXIMUM ********

UNITS MINIMUM AVERAGE MAXIMUM ******** 0 ******** .. NAMEffiTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that lhis document and all attachments were prepared under my CLZ direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of lhe person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, lrue, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used 9A 0 TELEPHONE DATE 423 843-6700 08 08 14 I AREAl NUMBER YEAR MO DAY CODE Page 1 of 1 ! PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L 20QQ_ ___________ _ -__ 20DDY-DAISY _ _IN 3738!_ ______ _ Facilitv_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!L _!jAMIL TOl:!...COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPD£S) DISCHARGE MONITORING REPORT (DMRI TN0026450 117 G YEAR From I os I --I --I MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT NO DISCHARGE CJ *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION J NO. SAMPLE TYPE UNITS MINIMUM AVERAGE MAXIMUM UNITS ******** 0 FLOATING (SEVERITY) .. ******** 9A 0 0 0 0 ENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer 423 843-6700 08 08 14 information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY TYPED OR PRINTED CODE_ -------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Add res.§_ _E 20QQ_ ___________ _ -__ _§.ODDY-DAISY _ _JN 37381.._ ______ _ Facility_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!!_ .J::!AMIL TO.!:!_ COUNTY __________ _ ATIN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ TN0026450 118 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD From I I To I I I MAJOR (SUBR 01) F-FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completin!l this form. QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. I FREQUENCY/ SAMPLE EX OF TYPE PARAMETER AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM 50050 (DO) 0 0 GROSS VALUE 0 0 EFFLUENT GROSS VALUE ********

NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used ********

                • Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT UNITS 19 TELEPHONE DATE 423 I 843-6700 08 08 14 AREA I NUMBER YEAR MO DAY CODE Page 1 of 1 S58 080911 800 -NPDES CORRESPONDENCE September 11, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement

& Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR AUGUST 2008 Enclosed is the August 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, \ ; . *-* 1'1* .4 / ** ( 1 l/l >--Q'lA.)cu. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, BR 4T-C d A. A. Ray, WT 7C-K J.D. Smith, OPS 4A-SQN G. R. Signer, WT 6A-K EDMS, WT CA-K (Enclosure) DMR0808.doc Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 September 11, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR AUGUST 2008 Enclosed is the August 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 ___ _§_ODD.Y .. : DAISY _ _IN 3738i._ ______ _ PERMIT NUMBER DIFFUSER DISCHARGE Facility_ ...I:JA *...§!:OUOYAH NUCLEAR PLANT _____ _..:. Locatio.!l_ .......!::!AMILTOH_COUNTY_ _________ _ MQ..NITORING PERIOD EFFLUENT I I J To ATTN: Stephanie A. Howard From NO DISCHARGE NOTE Read instructions before completinQ this form. D ... 50050 PARAMETER WATER DEG. 0 0 FF. BETWEEN SAMP. & M DEG.C s 0 0 0 EFFLUENT GROSS VALUE UNITS MINIMUM ******** .. MAXIMUM AVERAGE UN QUALITY OR CONCENTRATION I NO. I FREQUENCY! SAMPLE EX OF TYPE ANALYSIS 0 ******** 29.8 31 I 31 I MODELD 04 GRAB NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance w1th a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information subm1tted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 08 09 11 informatton, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalttes for submttting false SIGNATURE OF PRINCIPAL EXECUTIVE I mformation, tncluding the possibility of fine and imprisonment for knowing vtolations OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY TYPED OR PRINTED CODE -----_L__ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3199) editions may be used Page 1 of 2 DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 08/20/2008 @ 1 040 <0.10 mg/1 08/22/2008 @ 1551 JAB TN EPA 8015 CCW CHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 08/20/2008 @ 1 035 <0.10 mg/1 08/22/2008 _@ 1603 JAB TN EPA 8015 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L _E Q._BOX 2000 ___________ _ -__ _§.ODDY-DAISY _ _JN 3738L_ ______ _ Facility_ .-JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!)_ _!:!AMIL COUNT!:_ _________ _ ATTN: Stephanie A. Howard PARAMETER RINE, TOTAL RESIDUAL 0 0 GROSS VALUE NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR I YEAR I MO I DAY From I 08 To 08 08 31 MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT Form Approved. OMB No. 2040-0004 NO DISCHARGE CJ *** NOTE Read instructions before completinq this form. NO. IFREQUE EX OF ANALYSIS MAXIMUM UNITS 0.034 19 0 NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEP'HONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel \ Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 08 09 information, the *Information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete

.. 1 am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I 11 i information, including the possibility of fine and impnsonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA l NUMBER YEAR MO TYPED OR PRINTED CODE I L_____ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured:

1. Biodetergent 73551 (max. calc. cone. was 0.017mg/L--Iimit 2.0mg/L)
2. MSW-1 01 (max. calc. cone. was 0.059mg/L--Iimit 0.2mg/L)
3. H-150M (max. calc. cone. was 0.039mg/L--Iimit 0.050mg/L)
4. H-150M (low detection level analytical method was <0 020mg/L--Iimit 0.050mg/L)

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ ___ __§_ODD.Y_- DAISY _ _IN 3738L ______ _ Facility_ .3:/A -..§l:QUOYAH NUCLEAR PLANT _____ _ Locatio_Q_ .J:!AMIL TO_N_COUNTY._ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMR) TN0026450 101 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD From YEAR DAY I I YEAR I MO DAY 08 o:LJ To I 08 08 31 MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 BIOMONITORING FOR OUTFALL 101 EFFLUENT NO DISCHARGE [] ... NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. I FREQUENCY EX OF SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM STATRE 7DAY CHR RIODAPHNIA 0 0 0 0 ENT GROSS VALUE ********

NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision tn accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitled. Based on my inqutry of the person or persons who manage the system, or those persons directly responsible for gathering the tnformation. the information submitted IS , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are s1gn1ficant penalties for submitting false information, including the possibility of fine and imprisonment for know1ng VIolations. TYPED OR PRINTED ----------


COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in August 2008. EPA Form 3320-1 (REV 3/99) Previous editions may be used ********

Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT ---------ITS ANALYSIS 23 TELEPHONE DATE 423 843-6700 08 09 11 I YEAR MO DAY E Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ AddresL _.E.Q,_BOX_19QQ_ ___________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) TN0026450 103 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 -__ 2.0DDY-DAISY _ _IN 373BL ______ _ PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Facility _ _IYA -_§!:QUOYAH NUCLEAR PLANT _____ _ Locatio!l_ .J::!AMILTOl::!_COUNT:L_ _________ _ MONITC RING PERIOD ! EFFLUENT ATIN: Stephanie A Howard From YEAR I MO I DAY I YEAR I MO I DAY o8 I o8 I 01 To I 08 I 08 I 31 1 NO DISCHARGE LJ *** PARAMETER PH i;:>ULIU;:>, TOTAL SUSPENDED 0 0 NDUIT OR THRU PLANT 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P Cleary Site Vice President TYPED OR PRINTED QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM ********

7.5 1 Certify under penalty of law that thts document and all attachments were prepared under my directton or supervision in accordance wtth a system destgned to assure that qualrfied personnel properly gather and evaluate the 1nformat1on submilled Based on my inquiry bf the person or persons who manage the system, or those persons dtrect!y responstble for gathenng the 1nformalion, the information submilled is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are sign.lficant penalties for submitting false mformation, 1ncluding the possibility of fine and imprisonment for knowing violations. COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used NOTE: Read instructions before completinq this form AVERAGE ******** TELEPHONE DATE ----Principal Environmental Engineer 423 843-6700 08 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved OMB No. 2040-0004 Addres.L _f.Q,_BOX_1900 ___________ _ TN0026450 -__ _§_ODD.Y_- DAISY _ _IN 3738L ______ _ Facility_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l_ JjAMIL TOli_COUNTY __________ _ ATTN: Stephanie A Howard PARAMETER PH 0 0 r;:suLru;:s, TOTAL SUSPENDED 0 0 0 0 FFLUENT GROSS VALUE RON, TOTAL (AS FE) , IN CONDUIT OR THRU ENT PLANT 50050 1 0 0 EFFLUENT GROSS VALUE YEAR From I os I --I -* I AVERAGE ******** NAMErTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that thiS document and all attachments were prepared under my direction or in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted IS, to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are sign1f1cant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 3199) Previous editions may be used 107 G DAY 31 METAL CLEANING WASTE POND EFFLUENT NO DISCHARGE I XX I *** NOTE: Read instructions before completinq this form. QUALITY OR CONCENTRATION Cv Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE 423 843-6700 08 AREA NUMBER YEAR CODE SAMPLE TYPE DATE 09 11 -MO DAY Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L ___________ _ -__ _§.ODD.Y_* DAISY _ _IN 3738i_ ______ _ Facility_ .:JYA ._g:QUOYAH NUCLEAR PLANT _____ _ Locatio.!}_ _!jAMIL TOJ::LCOUNTY __________ _ ATTN: Stephanie A Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 PERMIT NUMBER DAY 31 MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. )FREQUENCY EX OF ANALYSIS SAMPLE TYPE ERATURE, WATER DEG. ENTIGRADE z 0 0 INSTREAM MONITORING PH 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 0 0 NDUIT OR THRU PLANT 0 0 HLORINE, TOTAL RESIDUAL 50060 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ********

04 04 I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE I direction or supervision in accordance with a system designed to assure that qualified personnel I Timothy P. Cleary properly gather and evaluate the InformatiOn submitted. Based on my 1nquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 I 843-6700 08 09 information, the information submitted IS, to the best of my knowledge and belief, true, Site Vice President accurate. and complete I am aware that there are significant penalties for submttting false SIGNATURE OF PRINCIPAL EXECUTIVE Information, including the possibility of f1ne and impnsonment for know1ng violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE i -COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used _____L_ Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Add res§_.£. ___________ _ -__ _§.ODD.Y.: DAISY _ _JN 3738L ______ _ Locatio.!l.._ _!iAMIL TQ_!i_COUNTL _________ _ ATTN: Stephanie A. Howard PARAMETER IC25 STATRE ?DAY CHR CERIODAPHNIA 1 0 0 TN0026450 YEAR From I os I --I -. I QUANTITY OR LOADING MAXIMUM UNITS MINIMUM ******** NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my Timothy P. Cleary direction or supervision 1n accordance with a system designed to assure that qualified personnel properly gather and evaluate the information subrmtted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the informat1on, the Information subm1tted is, to the best of my knowledge and bel1ef, true, Site Vice President

accurate, and complete.

I am aware that there are Significant penalties for submitting false mformatJon, including the possibility of fine and Imprisonment for knowing violations TYPED OR PRINTED COMMENTS AND OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used 110 T DAY 31 RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** NOTE: Read instructions before completinq this form. QUALITY OR CONCENTRATION I NO. I! FREQUENCY! SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS

23 UAd TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 09 11 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Addres_L .£. ___________ _ _________ _ _ _ _ 20DDY-DAISY _ _]N 3738i_ ______ _ Facility_ .JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l._ .J:jAMIL TOJi..COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 116 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard NO DISCHARGE CJ *** YEAR DAY From I 08 31 NOTE Read instructions before completinq this form. PARAMETER NO. IFREQUENCYI SAMPLE EX OF TYPE ANALYSIS IUI:Ct(.J;:), FLOATING (SEVERITY) 0 1 I 31 VISUAL 1 0 0 UENT GROSS VALUE 0 0 ENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my )Cu.c I TELEPHONE DATE direct1on or supervision in accordance w1th a system designed to assure that qualified personnel , 09 111 Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inqwy of the person or persons who manage the system, or those persons directly responsible for gathering the Princ1pal Environmental Engineer 423 843-6700 08 information, the Information submitted 1s , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are sigmficant penalt1es for subm1tting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA l NUMBER YEAR MO I DAY TYPED OR PRINTED CODE j *----------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Addres.§_ ___________ _ -__ _§.ODD.::L-DAISY _ _Iti]7381_ ______ _ Facility_ JYA-SEOUOYAH NUCLEAR PLANT _____ _ Locatio.!}_ .J::!AMIL TOl::LCOUNTY._ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 117 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 NO DISCHARGE D *** ATTN: Stephanie A. Howard YEAR DAY From I 08 31 NOTE: Read instructions before completinQ this form. NO. I FREQUENCY EX OF ANALYSIS PARAMETER 0 I 1 I 31 IL AND GREASE VISUAL 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law lhat this document and all attachments were prepared under my TELEPHONE DATE direction or supervis1on m accordance with a system des1gned to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted Based on my tnquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer 423 843-6700 08 09 11 1nformat1on, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete I am aware that there are significant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE I I information, including the possJbtl1ty of fine and 1mpnsonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA I NUMBER .YEAR! MO DAY TYPED OR PRINTED I CODE ------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes.

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 ' PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Addres,L ___________ _ -__ _§_ODD..Y_- DAISY _ _IN 37381_ ______ _ Facility _ _s:.JA-SEOUOYAJ:!.NUCLEAR PLANT _____ _ Locatioll_ .J::!AMIL TOJ:':!_COUNTf __________ _ ATIN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDE$) DISCHARGE MONITORING REPORT (OMR) TN0026450 118 G PERMIT NUMBER DISCHARGE NUMBERl MONITORING PERIOD YEAR DAY From 08 To 31 MAJOR (SUBR 01) F-FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. /FREQUENCY/ SAMPLE EX OF TYPE , DISSOLVED (DO) 0 0 IDS, TOTAL SUSPENDED 0 0 , IN CONDUIT OR THRU ITCI:I\TUENT PLANT 0 0 EFFLUENT GROSS VALUE NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Site Vice President TYPED OR PRINTED AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM ********

""' ""w ",_ '"" '"" '" "' -* ""oo """" m' , , 0 .4.u, d direction or supervision in accordance a system desighed to assure qualifted personnel " _

  • l.,...LQ_

. (j1A._. properly gather and evaluate the 1nformat1on submitted Based on my 1nqU1ry of the person or *

  • personswho manage the system, or those persons directly respons1ble for gathenng the Principal Environmental Engineer mformat1on, the informat1on subm1tted is , to the best of my knowledge and behef, true, accurate.

and complete. I am aware that there are signif;cant penalt;es for submitting false I SIGNATURE OF PRINCIPAL EXECUTIVE Information, including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT ----------------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used UNITS ANALYSIS 19 TELEPHONE DATE f----423 I 843-6700 08 09 11 AREA l NUMBER YEAR MO DAY CODE Page 1 of 1 ,. S58 081014 800-NPDES CORRESPONDENCE October 14, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR SEPTEMBER 2008 Enclosed is the September 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, BR 4T-C A. A. Ray, WT 7C-K J.D. Smith, OPS 4A-SQN G. R. Signer, WT 6A-K EDMS, WT CA-K (Enclosure) DMR0809.doc Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 October 14, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Sect1on 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR SEPTEMBER 2008 Enclosed is the September 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Su1te 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR! MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 ______ _ Facilitv _ _EA-gQUOYAH NUCLEAR PLANT _____ _ Locatio..!}_ _I:!AMIL TOli_COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER FF. BETWEEN SAMP. & DEG.C s 0 EFFLUENT GROSS VALUE PH 00400 0 0 EFFLUENT GROSS VALUE TOTAL SUSPENDED , IN CONDUIT OR THRU NT PLANT 50050 1 0 0 EFFLUENT GROSS VALUE TN0026450 PERMIT NUMBER YEAR From1 08 I --QUANTITY OR LOADING AVERAGE MAXIMUM UNITS ********

101 G MINIMUM AVERAGE ********

DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE D *** NOTE: Read instructions before this form. NO. IFREQ EX I OF ANALYSIS I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER II Cert1fy under penally of law that th1s document and all attachments were prepared under my I TELEPHONE i DATE I direction or supervision m accordance with a system des1gned to assure that qual1f1ed personnel Timothy P. Cleary properly gather and evaluate the 1nformat1on subm*tted. Based on my 1nqwy of the person or i I persons who manage the system, or those persons responsible for gathering the Principal Environmental Engineer 423 843-6700 08 10 . . . information, the information submttted is , to the best of my knowledge and belief, true, Site Vice PreSident

accurate, and complele.

1 am aware that there are sigmficant penaltiesfor subm1tting false SIGNATURE OF PRINCIPAL EXECUTIVE I tnformation, tncluding the posstbility of ftne and tmpnsonment for knowtng vtolat1ons. OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO I TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2 i I I 14 DAY DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Dateffime Collected Hydrocarbons Analysis Dateffime Analyst Method 09/10/2008@ 1105 0.28 mg/1 09/12/2008 @ 1552 KLM TN EPA 8015 CCWCHANNEL Extractable Petroleum Dateffime Collected Hydrocarbons Analysis Dateffime Analyst Method 09/10/2008 @ 11 00 <0.10 mg/1 09/12/2008 @ 1604 KLM TN EPA 8015 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Add res.§_ .J: ___________ _ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 101 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 _ _ _ _§_ODDY-DAISY _ ___IN 3738£._ ______ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facilitv_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ LocatiO.IL ..J::!AMIL TOli_COUNTY __________ _ EFFLUENT MONITORING PERIOO ATTN: Stephanie A. Howard *** NO DISCHARGE [J *** YEAR DAY From I 08 To 30 NOTE: Read instructions before completinq this form. PARAMETER NO. IFREQUENCYI SAMPLE EX OF TYPE ANALYSIS LORINE, TOTAL RESIDUAL 0 0 RE-C, RATE OF 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direct1on or superviSIOn in accordance with a system des1gned to assure that qualified personnel "E Timothy P. Cleary properly gather and evaluate the Information submitted. Based on my 1nquiry of the person or persons who manage the system, or those persons d1rectly responsible for gathering the Principal Environmental Engineer 423 I 843-6700 08 information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President and I am a_ware that there a:e penalties_ for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE i (formation. includmg the poss1b1l1ty of fine and 1mpnsonment for know1ng v1olations OFFICER OR AUTHORIZED AGENT AREA l NUMBER JYEAR MO I DAY_ TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured:

1. Biodetergent 73551 (max. calc. cone. was 0.018mg/L--Iimit 2.0mg/L)
2. MSW 101 (max. calc. cone. was 0.059mg/L--Iimit 0.2mg/L)
3. H-150M (max. calc. cone. was 0.038mg/L--Iimit 0.050mg/L)
4. H-150M (low detection level analytical method was <0.020mg/L--Iimit 0.050mg/L)

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2 I PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L ___________ _ ______ _ Facilitv _ _JYA-_.§!:QUOYAH NUCLEAR PLANT _____ _ Locatio.!]_ .J::!AMIL TOli_COUNTX_ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 Q PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR DAY From 08 To 30 MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE [] *** Form Approved. OMB No. 2040-0004 NOTE Read instructions before completinq this form. PARAMETER QUALITY oR coNCENTRATION I NO. 1 FREauENCYTI' -s-A--=M_P_L_E_ EX OF TYPE QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM BORON, TOTAL 01022 1 0 0 EFFLUENT GROSS VALUE ** .. > Z;;gEQt;llREMENl'<

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information subm1tted. Based on my inquiry of the person or persons who manage the system, or those persons d1rectly responsible for gathering the information. the Information submitted is , to the best of my knowledge and behef, true, Site Vice President

accurate, and complete.

I am aware that there are sigmficant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Boron was sampled on 07/02/2008@ 1122, on 07/16/2008@ 1215, and on 07/16/2008@ 1216. EPA Form 3320-1 (REV 3/99) Previous editions may be used <0.20 Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT UNITS ANALYSIS 19 o I 3192 TELEPHONE DATE 423 843-6700 08 10 14 I AREAl NUMBER YEAR MO DAY CODE Page 1 of 1 i PERMITIEE NAME/ADDRESS (Include Facilitv Name/Locat1on if Different) ___ _ Addres,L ___________ _ ______ _ Facility_ .I:JA -_g:QUOYAH NUCLEAR PLANT _____ _ Locatio.JL _HAMIL TO_!i_COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) Form Approved. OMB No. 2040-0004 TN0026450 101 T F-FINAL PERMIT NUMBER DISCHARGE NUMBERj BIOMONITORING FOR OUTFALL 101 MON ITOFillillYERlQ_D EFFLUENT NO DISCHARGE D ... From YEAR DAY 08 To 30 NOTE Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. EX SAMPLE TYPE IC25 STATRE ?DAY CHR CERIODAPHNIA 0 0 ?DAY CHR 0 0 NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Site Vice President AVERAGE MAXIMUM UNITS MINIMUM ********

I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance w1th a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my 1nquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the Information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are s1gnif1cant penalties for submitting false AVERAGE MAXIMUM UNITS ********

23 TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 10 14 SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA ! NUMBER YEAR MO DAY 1 TYPED OR PRINTED '--------- --COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in September 2008. EPA Form 3320-1 (REV 3/99) CODE I I Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Add res§_ ..f..Q_BOX_1900 ___________ _ TN0026450 -__ _§_ODDY-DAISY _ ______ _ Facility_ ....JYA -_g':QUOYAH NUCLEAR PLANT _____ _ Locatio.!:!_ _!::!AMIL TO.!:!_ COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER 0 0 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 00556 1 0 0 EFFLUENT GROSS VALUE ORTHRU PLANT 0 0 NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or superv1sion in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete I am aware that there are significant penalties for submitttng false information, including the possibility of fine and tmprisonment for knowing violations I TYPED OR PRINTED _:...._____

_________ -COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used LOW VOL. WASTE TREATMENT POND EFFLUENT NO DISCHARGE [] *** NOTE Read instructions before completinq this form. TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 10 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO CODE Page 1 of 1 14 DAY PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Addres_L _E ___________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMRJ MAJOR (SUBR 01) TN0026450 107 G F-FINAL Form Approved. OMB No. 2040-0004 -__ _§.ODDY-DAISY _ _IN 3738!_ ______ _ PERMIT NUMBER DISCHARGE NUMBER I METAL CLEANING WASTE POND Facility_ .JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!!_ _!:!AMIL TOli_COUNTY __________ _ EFFLUENT MONITORING PERIOD NO DISCHARGE @] ... From YEAR DAY 08 To 30 A TIN: Stephanie A. Howard NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE 0 0 1 0 0 ENT GROSS VALUE 1 0 0 EFFLUENT GROSS VALUE PHOSPHORUS, TOTAL (AS P) 00665 1 0 0 EFFLUENT GROSS VALUE 0 0 EFFLUENT GROSS VALUE IRON, TOTAL (AS FE) , IN CONDUIT OR THRU NT PLANT 0 0 ********

NAMErriTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the informat1on submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are sigmf1cant penalties for submittmg false Information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used ******** TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 10 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO I CODE Page 1 of 1 14 I DAY-j I PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res.§_ ___________ _ -__ _§_ODD ..X.: DAISY _ _IN 37381.._ ______ _ Facilitv_...I:JA-..§l':QUOYAH NUCLEAR PLANT _____ _ Locatio.!]_ JjAMIL TOJ:::!..COUNTY._ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMRJ TN0026450 110 G PERMIT NUMBER DISCHARGE NUMBER YEAR DAY From 08 30 MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. QUALITY OR CONCENTRATION I NO. IFREQUE EX OF PARAMETER SAMPLE TYPE RE, WATER DEG. z 0 0 0 0 DUITORTHRU PLANT 0 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL 50060 0 0 FFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Site Vice President I Certify under penalty of law that th1s document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly respons1ble for gathering the informat1on, the informatmn submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false MAXIMUM UNITS ANALYSIS 04 TELEPHONE DATE I Principal Environmental Engineer 423 I 843-6700 08 10 14 SIGNATURE OF PRINCIPAL EXECUTIVE i J 1nformat1on, Including the possibility of ftne and imprisonment for know1ng vtolations I OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO I DAY_ TYPED OR PRINTED I CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used PERMITTEE NAME/ADDRESS (Include Facifitv Name/Location if Different) ___ _ Addres_L ___________ _ _________ _ ___ _§ODDY-DAISY _ _IN 373BL ______ _ Facility_ JYA -_§E:QUOYAH NUCLEAR PLANT _____ _ Locatio_Q_ .J:!AMIL TOJi.COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) DISCHARGE MONITORING REPORT (OMR! TN0026450 110 T PERMIT NUMBER I I DISCHARGE NUMBER MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: Stephanie A Howard DAY 30 NO DISCHARGE I XX I *** NOTE: Read instructions before completinq this form. QUANTITY OR LOADING QUALITY OR CONCENTRATION / NO. I FREQUENCY! SAMPLE EX OF TYPE PARAMETER IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 0 0 !EFFLUENT GROSS VALUE lfie25 STATRE 7DAY CHR PIMEPHALES ITRP6C 1 0 0 EFFLUENT GROSS VALUE AVERAGE MAXIMUM UNITS ********

MINIMUM 45.2 i * *... AVERAGE *****"If** MAXIMUM ******** UNITS ANALYSIS 23 ., SEMI I.'C:PIIAPOS -CGMG!.c>S, -1 I IJ-. L .. : A /) j / ___ w.d __ I Ttmothy P Cleary !properly gather and evaluate the 1nformat1on submitted Based on my 1nqwy of the person or 1 .persons who manage the system, or those persons d1rectly responsible for gathenng the Prtnctpal Envtronmental Engineer 1 . l1nformat1on. the 1nforma\lon submitted 1s to the best of my knowledge and belief. I rue, --i 423 843-6700 08 : Stte Vtce President =-=['accurate. and complete I am aware that there are s'gn1f1cant penalt1es for subm1tt1ng false I--SIGNATURE OF PRINCIPAL EXE.CUTNE 1------===----c:-=--=:-c-==------


l:nformat1on,

nclud1ng the poSSibility of f1ne and 1mpnsonment for know1ng violat1ons II OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED , _ 10 COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Faci/itv Name/Location if Different)

___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 Addres_L ___________ _ -__ ....§.ODD..Y_- DAISY _ _]1::!.]7381._ ______ _ Facility_ .JYA -_§!:QUOYAH NUCLEAR PLANT _____ _ PERMIT NUMBER= I DISCHARGE NUMBER 116 G Locatio.!l._ _!:!AMIL TOJ::!...COUNTY __________ _ MONITORlNG PERIOD r YEAR 1 MO DAY To 08 09 30 NO DISCHARGE D ... ATTN: Stephanie A. Howard PARAMETER 0 0 FFLUENT GROSS VALUE IL AND GREASE VISUAL 0 0 ENT GROSS VALUE l_!iAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Timothy P. Cleary QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM ********

I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision 1n accordance with a system des1gned to assure that qual1fied personnel properly gather and evaluate the 1nformatton submitted Based on my inqUiry of the person or persons who manage the system, or those persons d1rectly responsible for gathering the 1nformat1on, the informatiOn submitted IS , to the best of my knowledge and bel1ef, true, I Site Vice President

accurate, and complete.

I am aware that there are Significant penalties for subm1tt1ng false r-------------------------1informat1on, Including the possibility of fine and imprisonment for knowing VIOlations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used NOTE: Read instructions before completinq this form. QUALITY OR CONCENTRATION I NO. IFREQUE EX OF AVERAGE MAXIMUM ******** 0 Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT UNITS 423 AREA CODE 9A ANALYSIS 0 Page 1 of 1 SAMPLE TYPE PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different! ___ _ AddresL ___________ _ -__ _§ODD_i_- DAISY _ _IN 37384_ ______ _ Facility_ JYA -_§!:.QUOYAH NUCLEAR PLANT _____ _ Locatio.!!_ _I:!AMIL TOl!.__COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) TN0026450 117 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD DAY To 30 MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT NO DISCHARGE [] *** Form Approved. OMB No. 2040-0004 ATIN: Stephanie A Howard NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM loesRIS, FLOATING (SEVERITY)

01345 0 0 EFFLUENT GROSS VALUE OIL AND GREASE VISUAL 84066 0 0 EFFLUENT GROSS VALUE QUALITY OR CONCENTRATION \ NO. I FREQUENCY EX OF ANALYSIS SAMPLE I TYPE I AVERAGE ******** MAXIMUM 0 R.ERORl :"1

UNITS 9A i +-------+-----t-----,----1 VISUAL 0 1/30 ';J,W$UA4. VISUAL NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary I Certify under penalty of law that th1s document and all attachments were prepared under my d1rection or supervision 1n accordance w1th a system des1gned to assure that qualified personnel properly gather and evaluate the InformatiOn submitted Based on my inquiry of the person or persons who manage the system, or those persons d1rectly responsible for gathering the information. the information submitted is . to the best of my knowledge and belief, true, I 1 Site Vice President r-TYPED OR PRINTED accurate, and complete. I am aware that there are significant penalties for submitting false informat1on, including the possibility of fine and 1mpnsonment for know1ng v1olat10ns. COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 AREA CODE 843-6700 NUMBER YEAR Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ .fu!dres.§_ ___________ _ -__ _§_ODD_1_- DAISY _ ____Tt:!]738i._ ______ _ Facility_ JYA -_§E:QUOYAH NUCLEAR PLANT _____ _ Locatio!]_ _ljAMIL TO_!::!_ COUNTY __________ _ ATTN: Stephanie A. Howard. NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT !DMR) TN0026450 118 G From MAJOR (SUBR 01) F-FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. I FREQUENCY I SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM OXYGEN, DISSOLVED (DO) ********

00300 1 0 0 1EFFLUENT GROSS VALUE FIDS, TOTAL SUSPENDED i 00530 1 0 0 EFFLUENT GROSS VALUE SOLIDS, SETTLEABLE 00545 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 0 0 EFFLUENT GROSS VALUE .:REPOFir**-,1 MGD NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this document and all attachments were prepared under my or superv1sion 1n accordance with a system designed to assure that quallf1ed personnel Timothy P. Cleary properly gather and evaluate the 1nformat1on submitted. Based on my inqUiry of the person or I persons who manage the system, or those persons d1rectty responsible for gathenng the . . . tnformation, the Information submitted is , to the best of my knowledge and belief, true, S1te V1ce PreSident

accurate, and complete 1 am aware that there are significant penalt1es for submitting false information, mcludmg the possibility of fine and 1mprisonment for knowing violations I TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

EPA Form 3320-1 (REV 3/99) Previous may be used ********

Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT UNITS ANALYSIS 19 TELEPHONE DATE -423 843-6700 08 10 14 I AREA l NUMBER I YEAR MO DAY CODE Page 1 of 1 S58 081113 800 -NPDES CORRESPONDENCE November 13, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR OCTOBER 2008 Enclosed is the October 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 28-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, BR 4T-C A. A. Ray, WT 7C-K J.D. Smith, OPS 4A-SQN G. R. Signer, WT 6A-K EDMS, WT CA-K (Enclosure) DMR0810.doc Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 November 13, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR OCTOBER 2008 Enclosed is the October 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington. D.C. 20555 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 ___ _.§.ODDY-DAISY _ ____IN 37381._ ______ _ Facility_ -..§E:QUOYAH NUCLEAR PLANT _____ _ Locatio.!}_ .J::!AMIL TO..!::!_ COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER

PERATURE, WATER DEG. NTIGRADE 00010 z 0 0 INSTREAM MONITORING TEMPERATURE, WATER DEG. CENTIGRADE 00010 0 0 EFFLUENT GROSS VALUE BETWEEN SAMP. & DEG.C s 0 0 0 0 0 ENT GROSS VALUE 0 0 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT

TN0026450 YEAR From I 08 I --I -* I UNITS MINIMUM ********

101 G DAY 31 DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE [] *** NOTE: Read instructions before completinq this form. QUALITY OR CONCENTRATION NO. EX AVERAGE MAXIMUM UNITS 0 ******** 26.4 04 NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 08 11 information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I Information, including the possibifity of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2 13 DAY DMR Attachment CCW Data CCWTRENCH Extractable Petroleum DaterTime Collected Hydrocarbons Analysis DaterTime Analyst Method 1 0/08/2008 (52 1154 0.36 mg/1 10/13/2008@ 1636 KLM TN EPA 8015 CCWCHANNEL Extractable Petroleum DaterTime Collected Hydrocarbons Analysis DaterTime Analyst Method 10/08/2008 @ 1151 0.10 mg/1 10/13/2008@ 1646 KLM TN EPA 8015 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT (OMRI MAJOR (SUBR 01) F *FINAL Form Approved OMB No. 2040-0004 Addres_L _E Q,_BOX.1.900 ______ -'-____ _ 101 G TN0026450 ______ _ DIFFUSER DISCHARGE Facilitv _ NUCLEAR PLANT _____ _ LocatiOJL __!:!AMIL TOJ:i.COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard From I os I . -I --I 31 NO DISCHARGE CJ *** YEAR DAY NOTE: Read instructions before completinq this form. UNITS NO. IFREQUE EX OF ANALYSIS PARAMETER RINE, TOTAL RESIDUAL 19 0 0 0 RE

  • C, RATE OF 0 _____________

. ___________


_______ -----* penaltyoflawthatthisdocumentandallattachmentswerepreparedundermy f--------------'--'----'---".c:__----11direction or super;1sion in accordance with a system designed to assure that qualified personnel gather and evaluate the informatiOn submitted.

Based on my 1nquiry of the person or' Site Vice President who manage the system, or those persons directly responsible for gathering the \information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false ! TVot:n f"'\o I information, including the possibility of fine and impnsonment for knowing violat1ons. COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) cipal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 843-6700 I 08 I 11 I 13 NUMBER IYEARI MO I DAY The following injections occured:

1. Biodetergent 73551 (max. calc. cone. was 0.021 mg/L--Iimit 2.0mg/L)
2. MSW 101 (max. calc. cone. was 0.061 mg/L--Iimit 0.2mg/L)
3. H-150M (max. calc. cone. was 0.041 mg/L--Iimit 0.050mg/L)
4. H-150M (low detection level analytical method was <0.020mg/L--Iimit 0.050mg/L)

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 2 of 2 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L ...£. Q.,_BOX 20QQ_ ___________ _ -__ _§_ODDY-DAISY _ _IN 37381._ ______ _ Facility_ .JYA -_g:QUOYAH NUCLEAR PLANT _____ _ locatio.n_ ....!:!AMIL TO.!i_COUNTY __________ _ ATIN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMRJ MAJOR (SUBR 01) Form Approved. OMB No. 2040-0004 TN0026450 101 T F-FINAL PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 MQNIIQB.J..f'.I.G_E'E RIOD EFFLUENT From YEAR I YEAR I MO I DAY 08 To 08 10 31 NOTE: Read instructions before completinq this form. NO DISCHARGE D ... PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION \ NO. \FREQUENCY\ SAMPLE AVERAGE MAXIMUM UNITS MINIMUM ********

>100.0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, includmg the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF AtiiY VIOLATIONS (Reference all attachments here) Toxicity was sampled October 26-31, 2008. EPA Form 3320-1 (REV 3/99) Previous editions may be used TYPE AVERAGE MAXIMUM UNITS ********

23 o. TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 11 13 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO I DAY CODE Page 1 of 1 November 13, 2008 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450,, COMPLIANCE TOXICITY TESTS, OCTOBER, 2008 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101, samples collected October 26-31, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated OutfalllOl and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment):

Sherrard, R. M., PSC IX-C Files, ER&TA, CEB IB-M SQN October 2008m TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE SUMMARY Report Date: November I3, 2008 I. Facility I Discharger:

Seguoyah Nuclear Plant I TV A 2. County I State: Hamilton I Tennessee

3. NPDES Permit#:

TN0026450

4. Type of Facility:

Nuclear-Fueled Electric Generating Plant 5. Design Flow (MGD): 1,579 6. Receiving Stream: Tennessee River (TRM 483.6) 7. IQIO: 3,49I 8. Outfall Tested: I 0 I 9. Dates Sampled: October 26-31, 2008 10. Average Flow on Days Sampled (MGD): I575, I555, 1554 1I. Pertinent Site Conditions: H-I50M was injected into the Raw Cooling Water (RCW) System starting on October 28, 2008 at 1120 and ended on October 31, 2008 at I255. 12. Test Dates: October 28-November 4, 2008 13. Test Type: Short-term Chronic Definitive

14. Test Species:

Fathead Minnows (Pimephales promelas) Daphnids (Ceriodaphnia dubia) I5. Concentrations Tested(%): Outfall I 01: Il.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephales promelas: UV treated Outfall I OI: I1.3, 22.6, 45.2, 72.6, 100 UV treated Intake: I 00.0 16. Permit Limit Endpoint(%): Outfall I 01: IC25 = 45.2% I7. Test Results: Outfall I 01: Pimephales promelas: IC25 > I 00% Ceriodaphnia dubia: > I 00% UV treated Outfall I 0 I: Pimephales pro me/as: > 100% Page 1 of96

18. Facility Contact:

Stephanie Howard Phone#: (423) 843-6713

19. Consulting I Testing Lab: Environmental Testing Solutions, Inc. 20. Lab Contact:

Jim Sumner Phone #: (828) 350-9364

21. TV A Contact:

Cynthia L. Russell *Phone#: (256) 386-2755

22. Notes: Outfall 101 samples collected Octqber 26-31, 2008, showed no toxic effects to fathead minnows or daphnids.

The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. *Page 2 of96 Fathead minnows were also exposed to tJv treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

METHODS SUMMARY Samples:

1. Samphng Point: Outfall 101, Intake 2. Sample Type: Composite
3. Sample Information:

Date Date Date (MMIDD/YY)/ (MM/DD/YY)/ Arrival Initial (MM/DD/YY)/ Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (OC) (mg/L) First Used By 101 I 0/26/08 0742 to I0/27/08 I456 1.3/2.1 t <0.10 I 0/28/08 1647 10/27/08 0642 I 0/29/08 1549 Intake I 0/26/08 0816 to 10/27/08 1456 1.7 <0.10 I 0/28/08 164 7 10/27/08 0716 10/29/08 1549 10I 10/28/08 0809 to 10/29/08 1423 0.8/1.5t <0.10 10/30/08 1630 10/29/08 0709 10/31/08 I550 Intake I 0/28/08 0826 to 10/29/08 1423 0.5 <0.10 10/30/08 1630 10/29/08 0726 10/31/08 1550 10/30/08 0802 to I1/01/08 1548 101 10/31/08 1352 1.8/2.8t <0.10 11102/08 1605 10/31/08 0702 11/03/08 I553 10/30/08 0834 to Il/0 1/08 1548 Intake 10/31/08 0734 10/31/08 1352 1.6 <0.10 Il/02/08 1605 11/03/08 1553 *TRC =Total Residual Chlorme tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation:

Samples from Outfall! 01 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath. Page 3 of96 Aliguots of Outfall 1 01 and Intake samples were UV through a SmartUV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Pimephales promelas Test Organisms:

1. Source: Aguatox, Inc. 2. Age: 24.55-24.78 hours old Test Method Summary:
1. Test Conditions:

Static, Renewal 2. Test Duration:

3. Control I Dilution Water: Moderately Hard Synthetic
4. Number of Replicates:

4 5. Organisms per Replicate: lQ 6. Test Initiation: (Date/Time) Outfall101 10/28/08 1647 ET UV Treated Outfall 1 01 10/28/08 1633 ET 7. Test Termination: (Date/Time) Outfall 101 11/04/08 1556 ET UV Treated Outfall 1 01 11/04/08 1540 ET 8. Test Temperature: Outfall I 01: Mean= 24.8°C (24.5 -25.1 °C} Ceriodaphnia dubia In-house Cultures <24-hours old Static, Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic 1 10/28/08 0800 ET 11/04/08 0705 ET Mean= 24.8°C (24.6 -25.1 °C} Test Temperature: UV-Treated OutfalllOl: Mean = 24.8°C (24.6-25.1 °C} 9. Physical I Chemical Measurements: Alkalinity,

hardness, total residual
chlorine, and conductivity were measured at the laboratory in each 1 00% sample. Daily temperatures were measured in one replicate for each test concentration.

Pre-and exposure test solutions were analyzed daily for pH and dissolved oxygen. 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific

Software, McKinneyville, CA). Page 4 of96 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1. Results of a Pimephales promelas Chronic/

7-day Toxicity Test. (Genus species) (Type I Duration) Conducted October 28-November 04,2008 using effluent from Outfall101. Test Percent Surviving Solutions (time interval used-(%Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 98 98 Intake 100 100 100 100 98 95 93 Test Solutions Mean Dry Weight (mg) (% Effluent) (replicate numbei} 1 2 3 4 Mean Control 0.707 0.655 0.717 0.672 0.688 11.3% 0.591 0.692 0.691 0.693 0.667 22.6% 0.664 0.709 0.613 0.650 0.659 45.2% 0.586 0.630 0.760 0.688 0.666 72.6% 0.657 0.617 0.577 0.724 0.644 100.0% 0.635 0.565 0.645 0.593 0.610 Intake 0.679 0.758 0.569 0.622 0.657 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso: TUc = 100/ IC2s Page 5 of96 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted October 28-November 04,2008 using effluent from Outfall101. Percent Surviving Test (time interval used -days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (%Effluent) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 35 36 34 34 31 33 35 33 33 33.3 11.3% 34 35 34 38 35 31 37 34 37 31 34.6 22.6% 37 38 34 36 36 36 37 35 37 31 35.7 45.2% 32 38 37 39 34 36 35 39 37 36 36.3 72.6% 38 40 39 37 34 35 39 36 38 38 37.4 100.0% 43 37 39 37 38 38 42 39 37 42 39.2 ICzs Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LC50: TUc = 100/ IC25 Page 6 of96 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted October 28-November 04, 2008 using water from Intake Percent Surviving Test (time interval used-days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 36 30 34 32 33 31 32 34 31 32.3 Intake 35 31 34 36 33 32 34 29 35 31 33.0 lCzs Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/ A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TU a = 1 00/LCso: TU c = 1 00/ I Czs Page 7 of96 TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets) 3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted October 28-November 04, 2008 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used-days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg) (% Effluent) replicate number) 1 2 3 4 Mean Control 0.489 0.416 0.440 0.502 0.462 11.3% 0.578 0.614 0.632 0.622 0.612 22.6% 0.503 0.489 0.604 0.606 0.551 45.2% 0.575 0.540 0.547 0.639 0.575 72.6% 0.558 0.579 0.663 0.688 0.622 100.0% 0.556 0.557 0.541 0.603 0.564 Intake 0.581 0.660 0.592 0.673 0.627 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso: TUc = 100/ ICzs REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) Species Date Time Duration Toxicant Results (IC2s) P. promelas October 28-November 04, 2008 1700 7-days KCl 0.79 giL C. dubia October 07 -14, 2008 0815 7-days NaCl 1.09 g/L Page 8 of96 PHYSICAL/CHEMICAL SUMMARY Water Chemistry Mean Values and Ranges for Pimephalespromelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant Effluent (SQN) October 28-November 4, 2008 Test Sample ID Temperature ("C) Initial Control 24.8 24.7 -24.8 11.3% 24.8 .., 24.7 -25.0 ..:! 24.9 " 22.6% :: 24.7 -25.0 "' .. ""' 24.9 .., 45.2% ..:: 24.7 -25.0 "' 24.9 e-72.6% .§ 24.8 -25.0 >l... 24.9 100.0% 24.8 -25.1 24.9 Intake 24.8 -25.0 Control 24.7 24.7 -24.9 24.8 11.3% 24.7 -24.9 24.8 "" 22.6% .... 24.7 . 25.0 24.8 0:: 45.2% 24.7 -25.0 "'l 24.8 . ;: 72.6% ;;; 24.7 . 25.0 (._) 24.9 100.0% 24.8 . 25.0 Intake 24.9 L___ ----24.7 . 25.0 Overall temperature ('C) Pimephales promelas Ceriodaphnia dubia Page 9 of 96 Final 24.7 24.6 -24.8 24.8 24.6 -25.0 24.6 24.5 -24.8 24.7 24.5 -24.9 24.8 24.7 -24.9 24.8 24.6 -24.9 24.8 24.6 -24.9 24.8 24.6 -25.0 24.9 24.7 -25.1 24.9 24.7 . 25.1 24.8 24.6 -25.0 24.8 24.6 . 25.0 24.8 24.7 . 25.0 24.8 24.7 . 25.1 ----------- Average 24.8 24.8 Dissolved Oxygen (mg!L) pH (S.U.) Initial Final Initial Final 7.9 7.5 7.64 7.37 7.7 -8.2 6.3 -8.0 7.41 -7.90 7.19 -7.51 8.0 7.5 7.63 7.35 7.8 -8.2 6.1 8.0 7.50 -7.90 710 -7.49 8.0 7.5 7.63 7.34 7.8 -82 6.2 -8.0 7.48 -7.91 709 -7.49 8.0 7.5 7.60 7.35 7.7 -8.2 6.3 -8.0 7.45 -7.88 7.15 -7.48 8.0 7.5 7.60 7.35 7.8 -8.3 6.4 8.0 7.45 -7.86 7.10 -7.50 8.1 7.5 7.59 7.35 7.9 -8.3 6.3 -8.0 7.45 -7.85 7.08 -7.52 8.1 7.6 7.58 7.35 7.8 -8.4 6.4 -8.0 7.42 -7.83 7.08 -7.53 7.9 7.9 7.64 7.52 7.7 -8.2 7.4 -8.2 7.41 -7.90 7.40 -7.66 8.0 7.9 7.63 7.54 7.8 . 8.2 7.3 -8.2 7.50 -7.90 7.41 -7.66 8.0 7.9 7.63 7.53 7.8 8.2 7.3 -8.2 7.48 . 7.91 7.40 -7.65 8.0 7.9 7.60 7.53 7.7 8.2 7. 3 8.2 7.45 . 7.88 7.39 . 7.64 8.0 7.9 7.60 7.52 7.8 8.3 7 2 8.2 7.45 -7.86 7.39 -7 65 8.1 7.9 7.59 7.52 7.9 8.3 7.3 -8.2 7.45 . 7.85 7.40 -7.66 8.1 7.9 7.58 7.51 7.8 . 8.4 7.3 7.42 -7.83 7.41 . 7.65 -----Minimum Maximum 24.5 25.1 24.6 25.1 Conductance Alkalinity Hardness Total Residual (f.lmhos/cm) (mg/L (mg/L CaC03) Chlorine (mg/L) 306 60 92 -296 -316 60 61 85 -96 289 ---277 -302 ------275 ---260 -284 --252 ---240 -263 ----224 ---207 -242 ---197 67 83 < 0.10 182 -215 66 -68 75 -92 < 0.10 -<0.10 204 68 81 < O.ID 177 -283 64 -74 79 -83 < 0.10 -<0.10 306 60 92 -296 316 60 -61 85 96 -289 ---277 -302 -. . . . 275 ---260 . 284 --252 ---240 263 -224 -. -207 242 . -197 67 83 < 0.10 182 . 215 66 . 68 75 . 92 < 0.10 -< 0.10 204 68 81 < 0.10 177 . 283 64 . 74 79 -83 < 0.10 . < 0.10 ' ' ' PHYSICAL/CHEMICAL SUMMARY Water Chemistry Mean Values and Ranges for Pimephales pro me las Tests, UV -treated Sequoyah Nuclear Plant Effluent (SQN October 28-November 4, 2008 Test Sample ID Temperature ("C) Initial Final Control 24.8 24.7 24.8 -24.9 24.6 -24.8 11.3% 24.9 24.9 "' 24.8 -25.0 24.6 -25.0 ..;:: 24.9 24.8 "' !: 22.6% 24.8 -25.1 24.6 -24.9 Q ... C)., 25.0 24.7 45.2% "' 24.9 -25.1 24.6 -24.9 25.0 24.8 & 72.6% .§ 24.9 -25.1 24.6 -24.9 25.0 24.7 100.0% 24.9 -25.1 24.6 -24.9 Intake 24.9 24.7 24.9 -25.0 24.6 -24.8 Overall temperature ('C) Pimephales promelas Average 24.8 Page 10 of96 Dissolved Oxygen (mg/L) Initial 8.0 7.8 -8.2 8.1 7.9 -8.2 8.1 7.9 -8.2 8.1 8.0 -8.3 8.1 7.9 -8.3 8.1 8.0 -8.3 8.1 7.8 -8.3 Minimum 24.6 Final 7.6 6.6 -8.1 7.6 6.6 -8.0 76 6.6 -8.0 7.6 6.7 -8.0 7.6 6.7 -8.0 7.6 6.6 -8.1 7.6 6.6 -8.1 Maximum 25.1 pH (S.U.) Conductance Alkalinity Initial Final (J.lmhos/cm) (mg/L CaCOJ) 7.61 7.40 294 60 7.43 -7.88 7.16 -7.57 280 -303 59 -61 7.62 7.37 288 -7.46 -7.87 7.15 -7.54 274 -300 --7.62 7.38 275 -7.46 -7.86 7.16 -7.55 266 -288 --7.61 7.37 253 -7.46 -7.85 7.16 -7.54 242 -263 --7.60 7.36 226 -7.46 -7.82 7.13 -7.55 217 -235 --7.60 7.36 193 70 7.46 -7.81 7.14 -7.57 181 -204 64 -80 7.58 7.37 189 71 7.45 -7.78 7.14 -7.57 170 -202 66 -80 Hardness (mg/L CaC03) 90 85 -----------83 79 -87 75 64 -81 SUMMARY I CONCLUSIONS Page 11 of 96 Outfall 101 samples collected October 26-31, 2008, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 1 00 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Appendix A ADDITIONAL TOXICITY TEST INFORMATION SUMMARY OF METHODS 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment.

Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution. DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
1. Pimephales promelas None 2. Ceriodaphnia dubia None Page 12 of96 PHYSICAL AND CHEMICAL METHODS 1. Reagents,
Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
2. Instruments:

All identification,

service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
3. Temperature was measured by SM 2550 B. 4. Dissolved oxygen was measured by SM 4500 0 G. 5. The pH was measured by SM 4500 H+ B. 6. Conductance was measured by SM 251 0 B. 7. Alkalinity was measured by SM 2320 B. 8. Total hardness was measured by SM 2340 C. 9. Total residual chlorine was measured by ORION Electrode Method 97-70. QUALITY ASSURANCE Toxicity Test Methods:

All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in giL KCl or NaCl. 2. Standard Toxicant:

Potassium Chloride (KCl crystalline) for Pimephales promelas. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia. 3. Dilution Water Used: Moderately hard synthetic water. 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses. Page 13 of96 REFERENCES

1. NPDES Permit No. TN0026450.
2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity ofEffluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002). 3. Standard Methods for the Examination ofWater and Wastewater, 20th Edition, 1998. 4. Quality Assurance Program:

Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version). Page 14 of 96 Page 15 of 96 Sequoyah Nuclear Plant Biomonitoring October 28 -November 4, 2008 Appendix B Diffuser Discharge Concentrations of Total Residual

Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 16 of 96 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall I 01) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion
Mollusks, During Toxicity Test Samphng, March 12, 1998 -October 31, 2008 PCL-401 mg/L Copolymer 0.005 0.011 0.021 0.019 0.015 0.015 0.015 0.024 0.024 0.024 0.024 0.024 0.024 0.023 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion
Mollusks, During Toxicity Test Sampling, Page 17 of96 Date 0 1131/2 000 .*. 02/01/2000 02/02/2000 02/03/2000
  • .. 02/04/2000

. 02/05/2000 02/06/2000 07/26/2000

07/27/2000 07/28/2000 07/29/2000 07/30/2000 07131!2000 08/01/2000 12111/2000 12112/2000 12113/2000 12114/2000

12115/2000 12116/2000 12117/2000 08/26/2001 08/27/2001 08/28/2001 08/29/2001 08/30/2001

08/31/2001 11/25/2001 11126/2001 11/27/2001 11128/2001 . 11129/2001 11130/2001 12/09/2001 ..* 12110/2001

  • ..* 12111/2001 12/12/2001

.* 12/13/2001 .* 12114/2001

  • ..
  • March 12, 1998-0ctober31,2008 PCL-401 mg/L Copolymer 0.009 0.028 0.009 0.009 0.009 0.009 0.009 0.019 0.019 0.018 0.019 0.019 0.019 0.019 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.021 0.021 0.021 0.020 0.021 0.020 0.02 0.019 0.019 0.02 0.02 0.02 0.02 0.02 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion
Mollusks, During Toxicity Test Sampling, Page 18 of96 Date 05/05/2002 05/06/2002 05/07/2002 05/08/2002 05/09/2002 05110/2002 08/04/2002 08/05/2002 08/06/2002 08/07/2002 08/08/2002 08/09/2002 10/06/2002 10/07/2002 10/08/2002 10/09/2002 10/10/2002 10111/2002 01112/2003 01113/2003 01/14/2003 01115/2003 01116/2003 01117/2003 04/06/2003

/ . .* .. 04/07/2003 04/08/2003 04/09/2003 04/10/2003 0411112003 March 12, 1998 -October 31,2008 PCL-401 mg/L Copolymer 0.02 0.014 0.014 0.014 0.023 0.023 0.023 0.008 0.008 0.02 0.02 0.019 0.02 0.019 0.018 0.018 0.019 0.019 0.018 0.018 0.018 0.018 0.018 0.018 0.019 0.020 0.020 0.020 0.009 0.021 0.021 0.021 0.021 0.022 Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, Page 19 of96 Date 06/15/2003 06/16/2003 06117/2003 06/18/2003 06/19/2003 .... 06/20/2003 . 08/03/2003 08/04/2003 . 08/05/2003 08/06/2003 08/07/2003

08/08/2003 10/05/2003

10/06/2003

10/07/2003

10/08/2003 10/09/2003 10/10/2003 March 12, 1998 -October 31, 2008 PCL-401 mg/L Copolymer 0.020 0.014 0.014 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.022 0.024 0.009 0.009 0.009 0.009 0.009 0.009 0.019 0.014 0.013 0.020 0.021 0.020 0.019 0.020 0.020 0.019 0.019 0.020 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall!

01) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion
Mollusks, During Toxicity Test Sampling, Date 11/07/2004 11/08/2004 11/09/2004 11/10/2004 11/11/2004 11/12/2004 02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/1112005 06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10/2005 07117/2005

07118/2005 07/19/2005 07/20/2005 07/21/2005 07/22/2005 10/30/2005 10/3112005 11/01/2005

11/02/2005 11/03/2005 11/04/2005 11/14/2005 11/15/2005 11/16/2005 11/17/2005 11/18/2005

11/19/2005 Page 20 of96 March 12, 1998 -October 31,2008 Date 11112/2006 11/13/2006 11114/2006 11115/2006 11116/2006 11/17/2006 11/26/2006 11/27/2006 11/28/2006 11/29/2006 11/30/2006 12/01/2006 05/28/07 05/29/07 05/30/07 05/31/07 06/01/07

06/02/07 12/02/07 12/03/07 12/04/07 12/05/07

12/06/07 12/07/07 04/13/08 04/14/08 04115/08 04116/08

04117/08 04/18/08 10/26/08 10/27/08

10/28/08 10/29/08 10/30/08 10/31/08 Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12,1998 -October 31,2008 Cuprostat-PF

  • mg/L Azole Page 21 of96 MSW 101 0.015 0.015 0.015 O.D15 0.015 O.D15 0.030 0.030 0.030 Page 22 of96 Sequoyah Nuclear Plant Biomonitoring October 28 -November 4, 2008 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

"'ti a BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 TV A Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 3 51 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General / J../ d_ /"' Chevy Williams: 'fA AJ tL_ Phone: 828-350-9364 Roy Quinn NPDES Number: TN0026450 7 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Dissolved Metals Collected. Samples remained on ice through out sampling and transport to lab. Field Identification I Grab/Comp. Collection Date/Time Container Flow 5058. ptLOj Sample Description Number& MGD Rain Event? Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-....... .......... ... ...... Inches Number Ten\p . a nee E\ (oC) t:\ SQN-101-TOX Comp 10/26/08-10/27/08 0742-2 (2.5gal) 1573-X 0010)'-:f.Ol I* 3/-z.\' 0642 1577 AVG= 1575 SQN-INT-TOX Comp 10/26/08-10/27/08 0816-1 (2.5 gal) NA X ()6;01(1-0Z. \ .1'(... a' ,J.SI... 0716 Sample Custody Fill In From Top Down -It $ ,Q, .... d Date/Tim()/ ..... A...Jl Relinquished By (Signature): Date/Time Received By (Signature): -Chevy Williams )WfA_ 10/27/08 I o: 3Dc50T Sonic Delivery 10/27/08 10 :;CJ EIJT , Sonic £. 10/27/08 10/27/08 ETS *11 Jl TK... "' ,..,_. -Jl...:> ,q 'S\c,. E:.\ 1'-\Sto E\ \..J I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. '"d TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Collected By: Chevy Williams, Roy Quinn BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 ofl Environmental Testing Solution, Inc. Delivered By (Circle One): 351 Depot Street. FedEx UPS Bus Client Asheville, NC Other (specify): Sonic Delivery \ 28801 General L Chevy Williams: )._

  • Phone: 828-350-9364 Roy Quinn Fax: 828-350-9368 Dissolved Metals Collected.

Samples remained on ice through out sampling and transport to Jab. Field Identification I Grab/Comp. Collection Date/Time Container Flow P!WJ O-J \YX)ss Rain Event? Sample Description Number& MGD Volume (Mark as Appropriate) Laboratory Use Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear---------.......... Inches Number Temp. ance 'C\ (OC) *,\ SQN-101-TOX Comp 10/28/08-10/29/08 0809-2 (2.5gal) 1551-X C(JII}U\Oi Q .. o ji.6'C o\ 0709 1558 \,_,,_?

  • AVG= 1555 SQN-INT-TOX Comp 10/28/08-10/29/08 0826-I (2.5 gal) NA X 00 1\)"Zt\.

uZ o.-0'v ?} +-0726 Sample Custody -Fill In From Top Down -i Relinquished By (Signature): Date/Time Received By (Signature): _....,._.. . -. a..r-n ueol '-""-rf 00 C(.. *"'

  • nl Ja-.. Date 1me Chevy Williams 10/29/08 oCJ5t EDI Sonic Delivery 10/29/08 0Cf51 6/JT ,r,;".Jj

-1. -;,(__ Sonic Delivery 10/29/08 ETS cl'\ 1AA. /{ 10/29/08 / .... -........ I'-\ 1.3 E\ \.._) I / Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. lit. BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 TV A Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Some Delivery Facility Sampled: Sequoyah NP 28801 General Commenr /J /; JI!J1 / Chevy Williams: J f J fJA. NPDES Number: TN0026450 Phone: 828-350-9364 Roy Quinn *_, . t:)._. eo-/V Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Dissolved Metals Collected. Samples remained on ice through out sampling and transport to lab. Field Identification I Grab/Comp. Collection Date/Time Container Flow p/V.)j ed*U: '5L>st3 Sample Description Number& MGD Rain Event? Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-........ (EDT) -*-........ Inches Number Temp. a nee (oC) E:\ SQN-101-TOX Comp 10/30/08-10/31108 0802-2 (2.5gal) 1551-X 00lb3!.0) /ojJec J-0702 1556 ,__. AVG= 1554 SQN-INT-TOX Comp 10/30/08-10/31/08 0834-I (2.5 gal) NA X (X3lD3IOZ.. /r & 'C a\

  • 0734 Sample Custody -Fill In From Top Down *-Relinquished By (Signature):

Date/Time Received By (Signature): ........ (9 IT Date Time c) Chevy Williams 10/31/08 IDOtGD(

  • Sonic Delivery 10/31108 loo/8{}-f Sonic Delivery 10/31/08 ETS *.1\. It-10/31/08

/:11 \ :,cs ?_ £\ r \ (_ E.-, r \._) I -... , J'-"1 :::>"'1 (/ Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. I I. I I ll ll I I I _, I I I I [ I Page: )T6 Chron ._\'\;holt: Effhent !'oxicity Test ;£PA-821-R-O:>l13 \lethod lUOO.!J Species: Pimepfwles promt!lils Client: TVA Facility: Seguoyah uclear Plant NPDES #: TN 0026450 Project #: __,S""'C§>,._,.__.._K _____ _ Dilution preparation information: Dilution prep (%) 11.3 22.6 Effluent volume (mL) 282.5 565 Diluent volume (mL) 2217.5 1935 Total volume (mL) 2500 2500 Test organism information: 45.2 1130 1370 2500 Organism age: 72.6 100 1815 2500 685 0 2500 2500 0\..t:) Date and times organisms '2.1* ocf t\oCO were born between: Organism source: t, C(p I C), 1.."\* 0 & Transfer bowl information: pH= Temperature= oc l.i1 2'-{.c.( Average transfer volume: County: Hamilton Treatment: Non-treated Outfall: 101 Comments: Test information: Randomizing template: Incubator number: 3C.. Artemia lot number: e:fs-ICcHW Total drying time: -z.l.\*1-\olll..! Date I Time in: \\ *IJ'-'-ot l\o 2.0 Date I Time out: \\-<lS-tt \\:,1..0 Oven temperature: (} :L_ Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, Control water batch used Sample numbers used Analyst feeding feeding time time 0 2 3 4 5 6 7 Control in.[ormation: Acceptance criteria Summary of test endpoints: % Mortality: o1. :-;20% 7-day LC50 ) l CCI.,. Average weight per initial larvae: I t"J.I.&[ NOEC JC01. Average weight per surviving larvae: £Lie &5 > 0.25 mg/larvae LOEC >10()7 .. ChV > 'oc7. IC2s >I 00 7o 1 Page 26 of 96 I I J I I I I I I Species: Pimephales promelas Date: _i._.D..__*_,-z.....,.f'-*=cJ;:o__ __ _ Client: TV A I Sequoyah Nuclear Plant-Non-treated Survival and Growth Data Day CONTROL 11.3% 22.6% A B c D E F G H I J K L 0 /0 to 10 to IO IO IO IO IO IO /0 10 1 It ID ID 10 /() ID 10 I 0 /0 I{) 10 10 2 IC /(J ID /0 /0 /0 IO IO 10 IO 10 /0 3 lo /O 10 10 (b 10 10 /0 /0 /0 10 JO 4 10 10 IO /0 /0 /0 /C 16 {() IO /0 IO 5 /6 /0 IO /0 f(J 10 IO IO I() /Q 10 IO 6 10 /Q /0 IO 10 10 /D I(J 10 ID 10 IO 7 ID It> 10 /0 IO IO /0 /0 to 10 IO 10 A = Pan weight (mg) Color 1'-!.18 identification: bla.d.<. IS.OI lt.80 \5.1'l IS.SI 15.0\o IS.S(p Analyst: LA-A B =Pan+ Larvae weight (mg) ?. 1.'-\l -z..\.00 z:z.l5 L.\ D .Z.k 7-t.\O 10.'11 7_21Z 20.41 L."ZJ,c\:; zz.ob Analyst: Larvae weight (mg) =A -B 7.Dl (;55 /,ll (p, 17. Sctl (o_qz. (,. G 3 7.01 Weight per initial number lo.1n D*i.oct'l 0.\o\3 ;D of larvae (mg) t;.16l O."'TZ.. p.(.,C\1... i]\ \ o.l-11 \,) = C I Initial number of larvae c. o. Average Percent weight per reduction ,3.\1 .. o.to-5'1 £.\. '27. initial from control (). b && o.lolo'"1 number of (%) larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Calculations and data reviewed: Comments: Page 27 of ll / I I I I I Sptcies: Pimi!ph,z!c>s promt'fas Client: TVA. Sequoyah

\udear Plan;-_';on-rreated Survival and Growth Data Day 45.2% 72.6% 100% M N 0 I p Q R s T u v w X 0 to ID 10 /0 10 /0 IO /0 10 10 /0 ID 1 /{) /D I D ID /0 IO /() /{) 10 I() /D /(J 2 /0 10 JD /0 ID 10 tO /0 IO 10 tO 10 3 10 10 10 10 10 /D /() 10 co 10 10 /0 4 to ID /C 10 10 lC I{) /0 10 ID iD 10 5 /{) to /D /() to ID /0 ID /() 10 10 /0 6 /0 /() 10 10 to /C 10 /(J /0 10 /()
r 7 "*** ILfr '1 10 ID 10 10 /0 to to fC /() IO {() A= Pan weight (mg) Color r{o'-# 11\.1.1. identification:

blOcK (!.{. '01 13.U. \3.\lt.f 1'1.'13 Analyst: L&0 (3.31 B =Pan+ Larvae weight (mg) ZO.'S"t; -z.l.ll ?.:z .3lo zo.(J) \Cj lct.B\ lq. Z:i_i:SI (0.\'Z. Analyst: u .u Larvae weight (mg) =A-B S,blo l,o. jO 1.loo \v.bb I lv. l,.\l S.ll 5J,5 Vt5 '5.03 Weight per initial number IJ!r;o -1\ilo ,.,a_ rJ-1 r \o\l c.-'lz.L.\ of larvae (mg) 0.96 t.iol\5 = C I Initial number of larvae V* (). o. 0 .w'O" o\D IJ. o.v Average Percent weight per reduction

3. ?7. O.I..'*N

\..Y 1. O.lo\(.) l\.l.H'. initial from control o.t.,Cob number of (%) larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: I I I I I I I I I I I I Pin-It!phaJ.::s promr:las D:.ne:


Client: TVA_, Sequoyah \udear Pl:am - Survival and Growth Data Day 100% Intake y ! z AA BB 0 /o /0 ID /0 I IO IO 10 2 J() 10 /0 ID 3 10 lb 10 10 4 /0 10 ro IC 5 IO 't ItA. F'Er 10 IC 6 IO IO g'cJ.Fl r 10 7 H .. _.. g\L.b IC A= Pan weight (mg} Color 63Y 14.10 13.C0t identification: lolQc.Jc Analyst: B =Pan+ Larvae weight (mg) bL_) LL.,\3 lCt!jO Analyst: Larvae weight (mg) =A-B s.ift vz-z l.S'b Weight per initial number i6 Ill -z 'L of larvae (mg) o.1.5 Q. = C I Initial number of larvae Average Percent weight per reduction o s1 4.'57. initial from control number of (%) larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Calculations and data reviewed: e}r-Comments: Page 29 of96 "tt w 0 0 ...., * '* .;;iif.7 Environmental Testing Solutions, Inc. Concentration(%) Initial oumberof harvae A 10 Control B 10 c 10 D 10 E 10 11.3% F 10 G 10 ll 10 [ 10 22.6% J 10 K 10 L 10 M 10 45.1% N 10 0 10 p 10 Q 10 72.6% R 10 s 10 T 10 u 10 100% v 10 w 10 X l 0 y 10 100°/o Intake z 10 AA 10 BB 10 Outfall101: Dunnett's MSD value: 0,0879 PMSD: 12.8 lnt11.ke: Dunnett's MSD value: 0.0836 PMSD: 12.Z Finlll number of larvae 10 10 10 10 10 10 10 10 10 10 10 \0 10 10 10 10 10 10 10 10 10 10 10 9 10 10 8 9 A"' Pu weight (mg) 14.40 14 45 15.01 14 92 14.35 14 18 12.80 15 79 14 27 15.57 15 06 I 5 56 14.52 14.87 14.76 13.72 1428 13.37 14 04 13.64 14 93 14.22 1536 14 19 15 34 14 70 13.81 14 04 TVA I Sequoyah Nuclear Plant, Outfall101-Non-treated October 28-November 04,2008 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Not for Compliance Assessment, lnternal Laboratory QC B"' Pan + Lan*ac weight (mg) Weight I Sunh*ing Men lteigbt/ Coefficient \o\oeigb1 (mg} .a umber uflarvae (mg) Surviving nf (Muaw-clthl , ... IJtn*ac (m£) ou*b"' 2!.47 7 07 0.707 21.00 6.55 0.655 0.688 4.2 22.18 7 17 0 717 21 64 672 0.672 20.26 5 91 0 591 21.10 692 0 692 0 667 7 6 19 71 6 91 0 691 22 72 6 93 0 693 20.91 6.64 0.664 22.66 7 09 0 709 0 659 60 21.19 613 0 613 22 06 6 50 0.650 20.38 5 86 0 586 21.17 6.30 0.630 0.666 11.3 22.36 7.60 0.760 20.60 6 88 0.688 20.85 6 57 0 657 19.54 6.17 0 617 0.644 9.7 19 81 5.77 0.577 20.88 7.24 0 724 21 28 6.35 0 635 19.87 5.65 0.565 0 626 67 21.81 6.45 0.645 20.12 5 93 0.659 22 13 6.79 0.679 22.28 7.58 0.758 0.710 4 9 19.50 5 69 0 711 20 26 622 0 691 Minimum Significant Difference Percent Mmimum Sign1ficant D1fference Weit:,bt/loitial Dumber uf lan'llC (mg) 0 707 0 655 0 717 0 672 0 59! 0.692 0 691 0 693 0 664 0 709 0 613 0 650 0.586 0.630 0.760 0.688 0.657 0.617 0.577 0.724 0 635 0 565 0 645 0 593 0.679 0 758 0.569 0 622 Mean sun*i'"' (%) 100.0 100.0 100.0 100.0 100.0 97.5 92.5 Project muHbt*.,*. f*** t v" ' ---*-------Mcll.nncil!hl/ Pcn:tut n:tlunion from lnitillloumhcr ur \llflill1ill\' _.1,, I ,,,,Hoi(%) tunuc (Ill!!) 1-' -+------0.688 -Ll ... +---*-******** l J! 0.667 0.659 0.666 0.610 0.657 i 1.6 [I() I -T *. 2 J.2 1--* ---t---------1 6.4 1.:. L ___ ..... -f------11 .. ---LS _.-l ... ___ _ PMSD is a measure of test precision. The PMSD is the minimum pe1cent difference between the control and treatment that can be dt!clared slg!l!li .... ,JJ)I i11 11 whok ... *ll)\1, 1l! tdxlct!y te:st Lower PMSD bound determined by USEPA (101h percen1ile) 12%. Upper PMSD bound de1ermined by USEPA (90th percenl!le) 30%. Lower and upper PMSD bounds were de1ermined from the I Oth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Va11abdoty StuJy (liSLI',\ 'IHJ 1 "* I I'U'A, cOO I b) USEPA 200 I a, 2001 b. Finai Report: Interlaboratory Variabthty Study of EPA Short-term Chronic and Acute Whole Effluent Toxic1ty Test Methods, Volumes 1 and 2-Appendix.' EPA-821-8-0 1-004 and EPA-821-B-Ol-005. US EnvJronnh:!ntul JlrutcCltun l'int:llllh-111, OH 1-C w ...... 0 ..... \0 0\ TVA I Sequoyah Nuclear Plant, OutfalllOl-Non-treated Environmental Testing Solutions, inc. Start Date: I 0/28/2008 Test ID: End Date: 11/4/2008 Lab ID: Sample Date: Protocol: Comments: Non-treated October 28 -November 04, 2008 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth PpFRCR Sample ID: ETS-Envir. Testing Sol. Sample Type: FWCHR-EPA-821-R-02-013 Test Species: TVA I Sequoyah Nuclear Plant, Outfall I 0 I DMR-Discharge Monitoring Report PP-Pimephales promelas Cone-% I 2 3 4 D-Control 0.7070 0.6550 0.7170 0.6720 -==-:-::-:c-:----":-=::c:-----:--:-=-:-:---------------------------------***--*----- 1!.3 0.5910 0.6920 0.6910 0.6930 22.6 0.6640 0.7090 0.6130 0.6500 45.2 0.5860 0.6300 0.7600 0.6880 72.6 0.6570 0.6170 0.5770 0.7240 100 0.6350 0.5650 0.6450 0.5930


Transform:

Untransformed !-Tailed Lsotuni,* Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Meiln N-Mcan D-Control 0.6878 1.0000 0.6878 0.6550 0.7170 4.236 4 0.6878 1.0000 1!.3 0.6668 0.9695 0.6668 0.5910 0.6930 7.575 4 0.576 2.410 0.0879 0.661,X ll. '/()'j') 22.6 0.6590 0.9582 0.6590 0.6130 0.7090 6.020 4 0.788 2.410 0.0879 0.6025 O.LJ(>i.i 45.2 0.6660 0.9684 0.6660 0.5860 0.7600 11.308 4 0.596 2.410 0.0879 0.6625 1).%.\j 72.6 0.6438 0.9360 0.6438 0.5770 0.7240 9.737 4 1.206 2.410 0.0879 0.6438 ll.LJ.\(>0 100 0.6095 0.8862 0.6095 0.5650 0.6450 6.112 4 2.145 2.410 0.0879 0.6095 0.!!81>2 Auxiliary Tests Statistic Critical Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.9664126 0.884 Bartlett's Test indicates equal variances (p = 0.67) 3.22156215 15.0862722 Hypothesis Test (l-tail, 0.05) NOEC LOEC ChV TU MSDu MS Do MSD MSE Dunnett's Test 100 >100 1 0.08789734 0.12780421 0.00283454 0.0026604 Treatments vs D-Control % 58.553 92.420 >100 >100 >100 >100 >100 sqnl (!I I 0-28.-UI:idaw '"d I:W w N c -. 1.0 0\ TV A I Sequoyah Nuclear Plant, Intake-Non-treated October 28-November 04,2008 Statistical Analyses \ ... J Environmental Testing Solutions, Inc. Larval Fish Growth and Survival Test-7 Day Growth Start Date: 10/28/2008 Test ID: PpFRCR Sample ID: End Date: 11/4/2008 Lab ID: ETS-Envir. Testing Sol. Sample Type: Sample Date: Protocol: FWCHR-EPA-821-R-02-0 13 Test Species: Comments: Non-treated Cone-% 1 2 3 4 D-Control 0.7070 0.6550 0.7170 0.6720 100 0.6790 0.7580 0.5690 0.6220 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat D-Control 0.6878 1.0000 0.6878 0.6550 0.7170 4.236 4 100 0.6570 0.9553 0.6570 0.5690 0.7580 12.320 4 0.715 Auxiliary Tests Statistic Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96301621 F-Test indicates equal variances (p = 0.13) 7.71728659 Hypothesis Test (!-tail, 0.05) MSDu MSDp TV A I Sequoyah Nuclear Plant, Outfall I 0 I DMR-Discharge Monitoring Report PP-Pimephales promelas !-Tailed Isotonic Critical MSD Mean N-*Mcdll 0.6R78 1.0000 1.943 0.0836 0.6570 0.'.15:\.i Critical 0.749 47.4672279 MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences Treatments vs D-Control 0.08358076 0.12152782 0.00189112 0.00370012 0.50 15206]*---**----- 1 .. (> .. -----*-----. Linear Interpolation (200 Resamples) Point % SD 95% CL(Exp) Skew 1C05 >100 IClO >100 IC15 >100 JC20 >100 IC25 >100 IC40 >100 ICSO >100 sqn!IJ/ _lO .'.ll OHdo!a "d (TQ ('!) w .w 0 '<) Environmental Testing Solutions, Inc. Concentration Control 11.3% 22.6% 45.2% 72.6% 100% 100% Intake TVA I Sequoyah Nuclear Plant, OutfalllOl -Non-treated October 28 -November 04, 2008 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project nuwhrr: 5058 Reviewed h); (-""--l£c.4.-

  • "'--

Pimeph,tf.:, .,.,Jinei!IS TVA S.::qu.;:c;h \ude:.H :)!ant--**Jn-'rt'Jted 11.3% 22.6% 45.2% 72.6% 100% 100% Intake 1 Page 34 of 96 I I I I I I I Spec:,;:-;: F:m"_*;lzaft'\ .).*;J*:"*ws Clien. TV.-\ . Seqc;. *. , h *, Jclear ?:dGt -

d Concen-Parameter tration I 11.3% Jt-c

.., "' ...... ,.,..,. -A ....... tte. A.N "'-'11:Cl:> .... I I I P1ge 1 of7 Chron.ic \\hole Effluent Toxicity Tesr tEPA-811-R-02-1)13 \IethoJ 1002 OJ Species: Caiodaphnia dubia Client: TVA Facility: Seguovah Nuclear Plant NPDES #: TN 0026450 Project#: ____ _ Dilution preparation information: Dilution prep (%) 113 22.6 Effluent volume (mL) 282.5 565 Diluent volume (mL) 2217.5 1935 Total volume (mL) 2500 2500 Test organism source information: 45.2 1130 1370 2500 Organism age < 24-hours old Date and times organisms were born \t!*21*08 between: Culture board A 72.6 100 1815 2500 685 0 2500 2500 115& T"' 1\o\'-\ '1)*1. \-646"1. Replicate number: lj2jlj4j5j6j7j8 9 ' Jil' Culture board cup number: . I -s I 1 o /l"l. I I '8 1*n I 'lR' Transfer bowl information: pH= 1."1'-\ su Temperature = 1. 'l .\ oc Daily renewal information: Day Date Test initiation, Control water batch used 0 2 3 4 5 6 7 Control information: 1 2 Acceptance criteria %of Male Adults: o7 .. 01. %Adults having 3'd Broods: Joo1. lex/f.. 80% %Mortality: D1. D1 .. County: Hamilton Treatment: Non-treated Outfall: 101 Comments: Test information: Randomizing template color* &...flP() ..._, Incubator number and shelf location: YWT batch: Selenastrum batch: Sample numbers used 2'&\ Analyst Summary of test endp_oints: 7-day LCSO )10<)7. NOEC 1()0 7, LOEC )1001.. Mean Offspring/Female: L5.0 offspring/female ChV 'l IOQ 1. %CV: .... 1..1. . <40.0% IC25 > 1007. Page 36 of96

1'):'; ":' ... I I Ceriodaphni b a () \\o 1\o Adult mortality L \._ \..._ \._ \._ L L \._ L L ' I 7 Young produced D l\ \S I--\ 1\ 1\ () 0 Total young produced 35 3\ 33 03 I Final Adult Mortality \.._ '--'-'-\._ \._ L. '-'-'-X for J'd Broods -;c... >'-><:. -,c._ Note: Adult mortal tty (L = l!ve, D = dead) Concentration: I CONC: 11.3% Survival and Reproduction Data I Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 0 () 0 0 0 I Adult mortality L L '-L L L L '--\...._ \._ 2 Young produced 0 0 0 0 0 0 0 a 0 0 Adult mortality L L L L L L L L L \...... I 3 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L-L L \..-\...... L L L L l_ 4 Young produced l.\ \o s s s '-I .s s L\ I Adult mortality L L L \... L-\_ L L \._ \_ 5 Young produced 1'-\ n .... t4 I"L \1... l'-\ t'-\ 1"2._ Adult mortality L L L L L L L L L \._ I 6 Young produced \\.p ,., 0 '"' 0 0 0 0 l\ 1$ Adult mortality L L L L L L L L L l 7 Young produced a D \\a Q_ l<i \J I (a 0 0 I Total young produced 3S 3! 3\ 3'-\ 31 ,... Final Adult Mortality \.._ L-'-'--\.._ L '--L \.._ L Note Adult mortality (L = hve, D =dead) I Concentration: % Mortality: (J7. Mean Offspring/Female: ..3 '1 . .,. r Page 37 of 96 % Reduction from Control: -.3.<<t 1. I I I I I I I I I I I I I I I I Page 38 of96 Cc"iudaphnia

luhia 1 Cliern:

\;uclear Plant-"<on<reated CO"iC 22.6% Survival and Reproduction Data Replicate number Dav 1 2 I J J 5 6 7 I 8 9 i 10 I 1 Young produced 0 0 C::> 0 01 (51 0 0 0 0 Adult mortality L L L L L_ L L '-'-\........ 2 Young produced a 0 [) () 0 (") 0 0 0 0 Adult mortality L L '--L '--'-L L L \..__ 3 Young produced 0 0 0 0 0 0 () 0 0 0 Adult mortality L L L \.._... L L L '-L. L. 4 Young produced 4 4 \o \...\ s \.o 5 s '-\ Adult mortality \.._. L L '-L. \.._. L. '--L L 5 Young produced \'S l.=, l"-\ -lS \3, -Adult mortality L-\.. L '--\._ \.._ L '-L \.._ 6 Young produced \.8 \'1 \\ \\ 0 \(\ D 1\ '" 1S Adult mortality L L \..._ L. \_ L. L \.......... '-'--7 Young produced 0 0 0 0 l8 0 llo 0 0 () Total young produced .3\o 3"\ 3S .3\ Final Adult Mortality '--L \.._ '-L L \...... '-L '--Note: Adult mortality (L =live, D =dead) Concentration: %Mortality: (j7 Mean OffsprinldFema1e: 3S*1 %Reduction from Control: _,. 7.1. CONC: 45.2% Survival and Reproduction Data Rtolicate number Day 1 2 3 4 5 6 7 8 9 10 l Young produced a 0 0 0 CJ 0 0 0 0 () Adult mortality \..._ L '-'-'--L '-'-'-'--2 Young produced n a () 0 () (\ 0 0 0 a Adult mortality L \,_.. L. L L L. L. L-L L 3 Young produced 0 0 0 0 () 0 0 0 0 0 Adult mortality L. '--L L L L \.._ L \..._ L 4 Young produced c...\ 5 s % s '5 ..., '--\ L\ Adult mortality L L L L L L L L L \._ 5 Young produced IS 1'2.. 1,3 II..\ 13 Adult mortality \._ \..._. L L L '-\..... L L '-6 Young produced \$ l\ 0 lq 0 l& l\o \'\ l'\ 1'\ Adult mortality \.._ L-L-L L-\__ L.. L L L 7 Young produced 0 0 l'\ 0 ,, 0 0 0 0 0 Total young produced

32. 211 3'-l 3Co 3'1 Final Adult Mortality L L '--'-L '---c.= \.... '--L Note: Adult mortality (L =live, D =dead) Concentration:

% Mortality:

07. Mean Offspring/Female:

.,31o. 3 %Reduction from Control: -q, o7. Specie c;: Caiodaphnia dubia Client. Sequovah 'luclear Plant* :<w-tre Survival and Reproduction Da! IS Adult mortality L L L \._ \._ \..... L L \._ L 6 Young produced \ i L.O tt\ 0 \\ \\ \'\ () "1.0 \& Adult mortality L_ \..._ \._ L L L L '-L '-7 Young produced 0 0 0 \'\ 0 0 0 \\ 0 0 Total young produced YD 3\ 3'5. 3c; 3\o 3t Final Adult Mortality '--L '-L \._ '--'-L L \..... . Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: O"t. Mean Offspring/Female: .31-... \ % Reduction from Control: -\2. 3"1. CONC: 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced 0 0 0 0 6 1'1 0 () () 0 Adult mortality L L L \._ L \....... \._ L L L 2 Young produced a D () 0 0 0 0 0 0 0 Adult mortality L L L L L L-L L L L._ 3 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L L L L L \_ L L \.._ 4 Young produced \.o '-\ b s s \.::, s .s lo Adult mortality L \._ '-L t_ L L L L \. 5 Young produced l\o lL\ 1"-\ l'-\ 14 1$ Adult mortality L L L L L L L L L L 6 Young produced 2.\ l'\ 1...0 D '" 'Z..O "2..0 L.\ Adult mortality L L \._ L L_ L._ \...._ L L L 7 Young produced 0 0 0 0 \8 0 0 0 0 0 Total young produced .31 3'\ 31 41.. 3'\ 31 Final Adult Mortality L L \,__ L L L L L L '--Note: Adult mortality (L =live, D =dead) Concentration: % Mortality: 0?. Mean Offspring/Female: ..3 2. %Reduction from Control: -n :1'7. 5 pecies: Ccriadap ,'z Iia dubia Client: SeguO'* 1h ,, uL!ear ?!ant **'\on-treated CO'iTROL-2 Survival and Ri!production Data -Replicate number Dav I 2 3 .t 5 6 7 i 8 I 9 i 10 1 Young produced 0 0 0 0 0 c 0 0 6 c Adult mortality L L L L '-L L. L L L_ 2 Young produced D 0 0 0 a 0 0 0 0 0 Adult mortality L L L L L L \..._ '-L '--3 Young produced 0 0 0 0 0 0 0 0 Q 0 Adult mortality '--'-L L L.. '-L '-'--'--4 Young produced

s 5 '-\ '-\ "-\ $ '-\ 1..\. \o '-\ Adult mortality L \.._ \.._ L '-'--'-'-\._. \_ 5 Young produced I\ L4 \"2.. n_ \1_ \\ \ \ Adult mortality

\._ L L L. '--\..__ \_ L '--\...... 6 Young produced II.\ 0 0 0 0 0 0 0 a -Adult mortality L L. '-L L L '--L \..... \._. 7 Young produced _D \\ \'-\ no \\o l'lo llo l"-'. 0 Total young produced 3\.o 3'Z. o1.... 3'\ Final Adult Mortality \....-L '--L L \.._ '-'-'--'---Note: Adult mortality (L =live, D =dead) Concentration: % Mortality: I D.,. Mean I coNe: 100% Intake Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 D D 0 0 a 0 0 D 0 Adult mortality L \_ L.. \_ L L L L L L 2 Young produced (") 0 0 0 a D a a_ 0 0 Adult mortality '-L L L L L L L L '---3 Young produced 0 0 0 0 (') 0 0 c 0 0 Adult mortality L L L L \..._. '--L '-L \.,_ 4 Young produced s '-\ y 4 s s q '-\ s '-\ Adult mortality L L L. L L L L L L L 5 Young produced \2. "-\\ \1-\"1... \£... \D \ '"Z.... Adult mortality '-L \._ L '--L L '-L L. 6 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L_ L L L-L L L_ L-L-7 Young produced \'5 \t\ \'\ \lo \S 18 \S \\ tS Total young produced 3'5 3'-' 3lo "Z.'\ 3\ Final Adult Mortality L '--'--L '---L L L. L '-Note: Adult mortality (L =live, D =dead) Concentration: % Mortality: (17. Mean Offspring/Female: I Page 40 of 96 %Reduction from Control:

  • 2.. 'l1.

""tj (1'1 ...... 0 -. '.C 0'\ Envlronm@ntal Tasting Solutions, Inc. Control-1 Day I 2 3 l 0 0 0 2 0 0 0 3 0 0 0 4 4 6 5 5 11 13 14 6 0 16 0 7 14 0 17 Total 29 35 36 11.3% Day I 2 3 I 0 0 0 2 0 0 0 3 0 () 0 4 4 6 5 5 14 12 13 6 16 17 0 7 0 0 16 Total 34 35 34 22.6% Day 1 2 3 l 0 0 0 2 0 0 0 3 0 0 0 4 4 6 4 5 IS 13 13 6 18 19 17 7 0 0 0 Total 37 38 34 45.2% Day I 2 3 I 0 0 0 2 0 0 0 3 0 0 0 4 4 5 5 5 13 16 13 6 15 17 0 7 0 0 19 Total 32 38 37 Replicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 4 6 4 4 13 13 13 12 17 0 0 0 0 15 14 17 34 34 3I 33 Replicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 5 4 5 14 12 12 14 19 0 0 0 0 18 15 18 38 35 3I 37 Replicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 6 4 5 6 13 14 12 I 5 17 0 19 0 0 18 0 16 36 36 36 37 Re licate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 5 4 5 15 12 14 14 19 0 18 16 0 17 0 0 39 34 36 35 TVA I Sequoyah Nuclear Plant, OutfalllOl October 28-November 04,2008 Verification of Ceriodaphnia Reproduction Totals 72.6% 8 9 10 Total Day 1 2 3 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 4 5 5 47 4 6 4 5 14 12 12 127 5 14 16 IS 0 16 16 65 6 18 20 19 17 0 0 94 7 0 0 0 35 33 33 333 I Total 38 40 39 100% I 8 9 10 Total Day 1 2 3 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 ' 0 0 0 0 0 3 0 0 0 5 6 4 49 4 6 4 6 13 14 12 130 5 16 14 14 0 17 15 84 6 21 19 19 16 0 0 83 7 0 0 0 34 37 31 346 Total 43 37 39 Control-2 I 8 9 10 Total Day 1 2 3 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 5 5 4 49 4 5 5 4 13 13 12 133 5 11 14 12 17 19 I 5 141 6 14 0 0 0 0 0 34 7 0 17 14 35 37 31 357 Total 30 36 30 100% Intake 8 9 IO Total Day 1 2 3 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 7 4 4 48 4 5 4 4 13 14 13 137 5 !? 12 11 19 19 19 142 6 0 0 0 0 0 0 36 7 18 15 19 39 37 36 363 Total 35_ 3I 34 Replicate number Total j 4 5 6 7 8 9 10-0 0 0 0 0 0 I) () I -I 0 0 0 0 () () () --j 0 0 0 0 () 0 () IJ 6 1----** .. 0\ 5 5 5 5 5 12 Tl -I 13 12 15 14 l:i ll<J () 17 17 19 0 20 IH I:IX 19 0 0 0 17 0 0 3o ! 37 34 35 39 36 3R 38 37-1 Replicate number .. I ' 4 5 6 7 8 9 10 Iota 0 0 0 0 0 0 () II --**-0 0 0 0 0 0 () II 0 0 0 0 0 0 I) I! ***---------* 4 5 5 6 5 5 6 S2 13 15 14 16 14 14 l'i II-i 20 0 19 20 20 18 21 177 18 0 0 t---0 0 () () IX _37 38 38 42 39 37 42 392 Replicate number l dial 4 5 6 7 8 9 10 -"-0 0 0 0 0 0 ,(\ :I 0 0 0 0 () I} () II 0 0 0 () 0 () r---*--0 () -* 4 4 5 4 4 6 4 14 12 12 II 14 13 II I ;:J *--0 0 0 0 0 0 Ill 30 16 16 16 16 14 15 (I [7.) 34 32 33 3I 32 34 31 32.1 Replicate number 9--r---w 'fulal 4 5 6 7 8 0 0 0 0 0 0 () II -0 **--** **-****-- 0 0 0 0 0 0 () 0 0 0 0 0 --)-*--() 0 () ****------*- -4 5 5 4 4 5 .:J 11 -13 12 12 12 10 1.1 I'J II'! *---------** 0 0 0 0 0 0 0 (I li-...... -----19 16 15 18 J 5 1) 167 _36 33 32 34 29 35 31 .no '"C ('> """ N 0 Environmental Testing Solutions, Inc. Concentration (%) 1 2 3 Control-1 29 35 36 11.3% 34 35 34 22.6% 37 38 34 45.2% 32 38 37 72.6% 38 40 39 100% 43 37 39 Control-2 30 36 30 100% Intake 35 31 34 Outfall101: Dunnett's MSD value: 2.195 PMSD: 6.6 Intake: Dunnett's MSD value: 1.615 PMSD: 5.0 TVA I Sequoyah Nuclear Plant, OutfalllOl October 28-November 04,2008 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses 4 34 38 36 39 37 37 34 36 MSD= PMSD= --t-t. -Project number: -T__ -----+'-5058 Replicate number Survival Average reproduction T oeffirl£:11 t of Pcrcclil reduction frtlHI (%) (offspring/female) \:anatiu11 JnHdul cuutrub CX,} 5 6 7 8 9 10 34 31 33 35 33 33 100 33.3 (,,2 Not applicable

    • ---35 31 37 34 37 31 100 34.6 6.8 -3.9 -*--**---------***-**-

36 36 37 35 37 31 100 35.7 :),(, -7.2 -------*----34 36 35 39 37 36 100 36.3 6.1 -'1.0 -----34 35 39 36 38 38 100 37.4 5.1 -12.3 --.---------------38 38 42 39 37 42 100 39.2 5.'1 -17.7 32 33 31 32 34 31 100 32.3 '*" :==L'"' .,,,,,,\<


  • -* ----------------**-33 32 34 29 35 31 100 33.0 (o.7 -2.2 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision.

The PMSD is the minimum percent difference between the control and treatment that can he uecl,ucd significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (I Oth percentile)

13%. Upper PMSD bound determined by USEPA (90th percentile)

47%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively. ofPMSD data trom FPA's WFT lnkr/,tbor"llltY Variability Study (USEPA, 200la; USEPA, 200lb). *-USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US LiiVlllllllilcllial Prokct1on Agency, Cincinnati, OH. USEPA. 2001 a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-u 1-00-1 and L'P;\ >L' I -ll-0 1-005. liS Environmental Protection Agency, Cincinnati, OH. r!) """ 0 .... \C 0'\ ) Environmental Testing Solutions, Inc. TVA I Sequoyah Nuclear Plant, OutfalllOl October 28-November 04, 2008 Statistical Analyses CeriodaEhnia Survival and Reproduction Test-Reproduction Start Date: 10128/200& Test ID: CdFRCR Sample ID: TV A I Sequoyah Nuclear Plant, Outfall I 0 I End Date: 1114/2008 Lab JD: ETS-Envir. Testing Sol. Sample Date: Protocol: FWCHR-EPA-&21-R-02-013 Comments: Non-treated Cone-% I 2 3 4 5 6 D-Control 29.000 35.000 36.000 34.000 34.000 31.000 11.3 34.000 35.000 34.000 38.000 35.000 31.000 22.6 37.000 38.000 34.000 36.000 36.000 36.000 45.2 32.000 3&.000 37.000 39.000 34.000 36.000 72.6 3&.000 40.000 39.000 37.000 34.000 35.000 100 43.000 37.000 39.000 37.000 3&.000 3&.000 Transfom1: Untransformed Cone-% Mean N-Mean Mean D-Control 33.300 1.0000 33.300 11.3 34.600 1.0390 34.600 22.6 35.700 1.0721 35.700 45.2 36.300 1.0901 36.300 72.6 37.400 1.1231 37.400 100 39.200 1.1772 39.200 Auxiliary Tests Kolmogorov D Test indicates normal distribution (p > 0.01) Bartlett's Test indicates equal variances (p = 0.99) Min Max 29.000 36.000 31.000 38.000 31.000 38.000 32.000 39.000 34.000 40.000 37.000 43.000 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV Dunnett's Test 100 >100 Treatments vs D-Control CV% 6.179 6.839 5.610 6.09& 5.073 5.&67 TU Sample Type: DMR-Discharge Monitoring Report Test Species: CD-Ceriodaphnia dubia 7 8 9 10 33.000 35.000 33.000 33.000 37.000 34.000 37.000 31.000 37.000 35.000 37.000 31.000 35.000 39.000 37.000 36.000 39.000 36.000 3&.000 38.000 42.000 39.000 37.000 42.000 -------J-Tailed Isotonic N t-Stat Critical MSD Mean

  • -*---------

10 36.08.1 I 0000 10 -1.355 2.287 2.195 36.083 I 0000 10 -2.501 2.287 2.195 36.08.1 I.OUOt) 10 -3.126 2.287 2.195 36.0H3 I.OUU!I 10 -4.272 2.2&7 2.195 36.0H3 1.!)0()() 10 -6.147 2.287 2.195 36.083 l.UOOO Statistic Critical Skew Kurl 0.66729027 1.035 -0.3926353 -0.1779355 0.62959719 15.0862722 MSDu MSDE MSB MSE *------*-* F-Prob dl' 2.19461764 0.06590443 43.1766667 4.60555556 1.8E-06 5. 54 Point % Linear Interpolation (200 Resamples) SD 95% CL Skew !COS >100 JC10 >100 1CI5 >100 IC20 >100 lC25 >100 IC40 >100 JC50 >100 sqn/IJ/ j().Ji)..{)Krlola "d :.;! (!) ... ... 0 ..... \0 Q'l TV A I Sequoyah Nuclear Plant, Intake October 28-November 04,2008 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reeroduction Start Date: 10/28/2008 Test ID: CdFRCR End Date: 11/4/2008 Lab ID: ETS-Envir. Testing Sol. Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Comments: Non-treated Cone-% 1 2 3 4 5 6 D-Control 30.000 36.000 30.000 34.000 32.000 33.000 100 35.000 31.000 34.000 36.000 33.000 32.000 Transform: Untransformed Cone-% Mean N-Mean Mean D-Control 32.300 1.0000 32.300 100 33.000 1.0217 33.000 Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.01) F-Test indicates equal variances (p = 0.71) Hypothesis Test ( 1-tail, 0.05) Homoscedastic t Test indicates no significant differences Treatments vs D-Control Min Max CV% 30.000 36.000 6.026 29.000 36.000 6.700 Sample ID: Sample Type: Test Species: 7 31.000 34.000 N 10 10 Statistic 0.97574127 1.29032254 8 32.000 29.000 t-Stat -0.751 MSDu MSDp 1.61535987 0.05001114 TVA I Sequoyah Nuclear Plant, Outfall I 0 I -lniak" DMR-Discharge Monitoring Report CD-Ceriodaphnia dubia 9 34.000 35.000 1-Tailed Critical 1.734 Critical 0.868 6.54108953 MSB 2.45 10 31.000 31.000 **-**----- holonic MSD Mean 32.650 1.615 32.650 Skew -0.0277877 MSE F-Prob 4.33888889 0.46210 I 0-1 I lll)ill) I IJI)IJ(I Ktiil -0.67-1,11(,5 ,_jj' I. I R Linear Interpolation (200 Resamples)


*---*---***

Point % SD 95%CL Skew IC05 >100 IC10 >100 IC15 >100 IC20 >100 JC25 >100 IC40 >100 1C50 >100 si.fniO/ /{I .:s IJSclula "'C (JCI (!) .!Jl 0 IIG .!) Environmental Testing Solutions, Inc. Control 11.3% 22.6% 45.2% 72.6% 100% TV A I Sequoyah Nuclear Plant, OutfalllOl -Non-treated October 28-November 04,2008 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily ChemicalAnalyses . P rojcd uu111bcr: 5058 H.cvicwcd It _1 : ' I ' ' I ' ' I I I I I I I I I I I ' Cerio./apnnuz d; bia Client: Senuo -ah '{ucJe,;_r Plant-Sv-rr*:Jted D!llA Clzem 11.3% 22.6% 45,2% 72.6% 100% 100% Intake f Page 46 of 96 I I I I I tration J 11.3% I 22.6% 45.2% 72.6% 100% 100% Intake Page 47 of96 Species: Ceriodaphnia dubia Client: Segw;vah \iuclear PlanT-'wn-treated Parameter Page-:--of7 Date: tO*Z&-oi .J. 1-c.,N.e..h, IN ,.)tJ:.,T u.Je.';;. C.OWFtf.Mth Chronic 'Ahole Efi1uent

  • oxi*:ity T"'sr *EP_\--321 K '.>J \fcthod lOi)l, Pimeplwles prumei,,-,.

Client: TVA Facility: Seguovah Plant NPDES #: TN 0026450 Project #: __._s ..... -..... cL..S.u8L- ___ _ Dilution preparation information: Dilution prep (%) !!3 22.6 Effluent volume (mL) 282.5 565 Diluent volume (mL) 2217.5 1935 Total volume (mL) 2500 2500 Test orJ?anism information: 45.2 1130 1370 2500 Organism age: 1-"" . M nuf'l C.. 72.6 1815 685 2500 0\l:) Date and times organisms /()*'Z.1*0cf lloOO were born between: 100 2500 0 2500 County: Hamilton Treatment: lTV-treated Outfall: 101 Comments: Each concentration was treated for 2 minutes with a UV sterilizer to remove pathogenic interferences. Test information: Randomizing template: .f>L.O*.. Incubator number: 2& l of6 Organism source: "TCX. &Ale.\\ {'p IC)* Artemia lot number: Transfer bowl information: pH= 1.81 Temperature = oc Total drying time: '2'1.1-\ Date I Time in: 1\ *o'-\-o8 11.'2.0 Average transfer volume: Date I Time out: lb'20 Oven lo()'( Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, Control water Sample numbers used Analyst feeding feeding time time 0 2 3 4 5 6 7 Control information: Acceptance criteria Summa__ry_ of_test endpoints: % Mortality: 07 .. $20% 7-dl!)l LCso > l (X}Gf Average weight per initial larvae: o.'i'-1.. '*: .

NOEC 100'1 Average weight per surviving larvae

> 0.25 mg/larvae LOEC "l tco7. ChV > (007. IC2s } I001o Page 48 of96 '. "';"(, ... .,, Species: Pimephafes promdas Dare: Client: TVA I Sequoyah Plant-Survival and Growth Data Day COl'i_TR()f-_ _11.3% A B _(:_ _p_ E _!'_ G _H I_ _.f K L 0 /0 ID IO (0 IO 10 /0 ID IO 10 lO ID 1 ID IO /() /D /r:J IO to Jo IO IO 10 10 2 ID 10 /D /C) IC {() IC 10 to /() 10 'o 3 /Q /D /D 10 IO /rJ lb to ID IO 10 10 4 to I 0 10 /{) lt) 10 ID 10 /0 /() /{) lO 5 /D /Q IO 10 10 /0 10 /0 /Q to ID 10 6 /0 /D Jo It> 10 JD to to IO IO I(J 10 7 ,t ... /0"' I OS.tl\ /D /() 10 /Q 10 10 IO ID A= Pan weight (mg) Color 1'-{.ulf identification:\ .\d.\l.C 13.1 s l&.tt Ill. \1. tt.f..S3 lll.11 ILI'l Analyst: B-Pan+ Larvae weight (mg) \Ct. '2-'\ *o. 'OO \4.)5 l't% -z: -\4.t.\'--\ Z...;.J<j 1<1. lio tt,.z3 z,o:lll Analyst: ! 0* Larvae weight (mg) =A-B l\.rh 1-\.\ \o l\.'-\0 S.t"l '::>. i \ q \0* . (pJl_ . 7 .. L. \g. 5.03 L-1. Vl ' 0'1 L,.O(p Weight per initial number n'-l.tF\

.. \.\\\o u.'*"o 0. io \'-\ 1"'.

oJoDL\ of larvae (mg) oS0l. c:;o') = C I Initial number of larvae V* V* D* \j.\.9 Average Percent weight per reduction '* o.SS\ - initial from control 0 .t.\lo '-O.l.>\2. number of (%) larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: &--Comments: Page 49 of96

Pimephal,<;

7!itn2/u'>

Clien :: T\--1. Sequoy:1h uck1r .?!ant-C\ -:rea ted Survival anrl Growth Data Day 45.2% 72.6% 100'% M N 0 p Q I R s T u I v I w X 0 10 /I';) /Q ID /() JO /Q 10 IC ID /D /0 1 /D f() /D 10 It> /() 1'0 I(J IO 10 JQ ID 2 j\) /0 /{) 10 {b /0 ID 10 /0 ID 10 /0 3 IO 10 10 lD /0 /D 10 to to 10 to 10 4 /Q /0 to fD lD 10 10 /(J /0 It> /0 10 5 /f) 10 IC ID J(J 10 /D 10 /(J 10 10 10 6 IO /C /{) ID /Q ID I(J It; /(j /{) ID 1\) 7 ID /0 /f) ID to /0 ID /D IO 10 tO 10 A= Pan weight (mg) Color llt'10 identification: l, btu.e 13.88 13.81 1l{.\H I'{ .1l\ \\{_(., 1 lS.33 12..Cf2. li,S(p 13.Z.S Analyst: UCH?, B =Pan + Larvae weight (mg) jCf.\o) Analyst: \0.(;-b IG.3'-I L.\."l.lJ iq.Tz. zo.% 2. \ .<1 (., Z.o}z. 18.'1B 7c.q3 I B.loi.? Larvae weight (mg) =A-B s:n s ..;\() s .... n 5.11 s5l 5;4\ (,_o') Weight per initial number t7:5q \ of larvae (mg) !r <;4"'1 oS'S'O o:"Slc\ O.lol93 .. , eSSie c55l = C I Initial number of larvae v. u. vu Average Percent weight per reduction o.S,'5 -1.'-\.lo io o.(."Z.L. -n.1.7 .. initial from control number of (%) larvae ( mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Calculations and data reviewed: L Comments: Page 50 of96 Page Species: Pimeph<Ifes D:irt: _ _(JL_ z . 04 __ --*---Client: TVA. Sequoyah

"iudear Plant-CV-trt:.tted Survival and Growth Data Day 100% Intake y i z I AA BB i 0 /0 Jo IO IO 1 /D 10 10 10 2 ICJ I() JO /0 3 10 IO IO 10 4 IO 10 /6 10 5 /(J /D /Q /0 6 lb /0 /(j /C) 7 /0 to /0 10 A= Pan weight (mg) Color I. l:llu.e identification:tt'T"l.

13.SLI 13.ttS \S. Analyst: LA1?J B =Pan+ Larvae weight (mg) iC/.35 'Z-Lib G.l.v'-\ Analyst: Larvae weight (mg) =A-B S.'6\-G.l:t' S97. . 13 \o. Weight per initial number i:),\o 13 of larvae (mg) o5'6\ o::s4 ..,_ = C I Initial number of larvae IJ. Average Percent weight per reduction initial from control o.< .. Zl -3$:1'7. number of (%) larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Calculations and data Comments: Page 51 of96 U1 N 0 ..., I,C .., .} ".:, Environmental Testing Solutions, Inc. Cuocentnttion (%) Rcplicutt: A Control B c D E 11.3% F G H I 22.6% J K L M 45.2% N 0 p _Q 72.6% R s T u 100% y w X y 100% Intake z AA BB Outfall 101: Dunnett's MSD value: PMSD: lntake: Dunnett's MSD value: PMSD: lnitial number of lan*ae 10 10 10 10 10 10 I 0 I 0 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 0.0791 17.1 0.0601 13.0 FinMI number of larvae 10 10 10 10 10 10 10 10 10 10 10 )0 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 A=-Pan weight (mg) 14.40 14.64 14.34 14 33 13 18 14 64 13.12 14 12 I 4.53 14.27 12.19 14.64 I 3.88 14.28 13 87 14.87 14.14 14.67 15.33 13.44 12 92 14 86 13 25 14.40 l3 54 13.65 15.84 14 31 PMSD-TVA I Sequoyah Nuclear Plant, OutfalllOl-UV-treated October 28-November 04, 2008 Pimephales prome/as Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Not for ConlPiiance Assessment, Internal Laboratory QC B = Pau + Lan'ae Lan*.ll.e\'"eight (mg) Weight I Surviviag Mcu\\cigbt/ Coefficiut of \'llrilitiun Weight /Initial number (mg) *A-B a umber oflarvac (mg} SurviYiag number of wdcht ,.., of lan**e (mg) lan*se(mg) 11\l,.b..rnrL.,-wu)(o/*) 19 29 4 89 0 489 0.489 18.80 4.16 0 416 0.462 0 416 8.8 I 8.74 4 40 0.440 0 440 I 9.35 5 02 0.502 0.502 18 96 5 78 0.578 0 578 20.78 6 14 0.6)4 0.612 3.8 0 614 19.44 6.32 0 632 0.632 20 34 6.22 0 622 o o22 19.56 5.03 0 503 0.503 19 16 4.89 0 489 0 551 0 489 18 23 6.04 0.604 11.5 0 604 20.70 6 06 0 606 0 606 19.63 5.75 0.575 0 575 19 68 5 40 0.540 0.575 7.8 0 540 19 34 5.47 0 547 0.547 21.26 6 39 0.639 0.639 19.72 5.58 0.558 0.558 20.46 5.79 0 579 0 579 0.622 I 0.2 21 96 6.63 0.663 0 663 20.32 6.88 0.688 0.688 18.48 5 56 0.556 0 556 20.43 5 57 0.557 0 557 18 66 0.564 48 5.41 0 541 0 541 20 43 6 OJ 0 603 0.603 19.35 5 81 0 581 0 581 20 25 6.60 0.660 0 660 0 627 7.5 21.76 5.92 0 592 0 592 21.04 673 0 673 0.673 Minimum Significant Difference Percent Minimum Significant Diffcrcricc (%) 100.0 100.0 I 00.0 100.0 100.0 100.0 100.0 Projec( uumlH'r: --r-y-- lttitialuumbcr ul !Mr\'itC 0.462 O.o 12 0.55 I 0.575 0.622 0.%4 0.627 ul cnt1cdudiun frum ronlrul t%) \afiatiuu 1.\J. .... )""I ,.( I** *4l] .. K.K !'\ 111 applicilhlc


1---*32.*1 . -11.' t 7.8 19.2

! --------- --*-r -* I 0.1 I -34.7 -* *--.J H -l.?..t -7.5 J;i.7 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the *control and treatment that can be declared statJstH.:ally 111 d wlHdc cllh.t;n\ !,!', . !I)' test Lower PMSD bound determined by USEPA (lOth percentile) I 2%. Upper PMSD bound determmed by US EPA (90th percentile)- 30%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratot-y Vat iabdity Snaiy (US EPA, :*uo I J. ( I' \ lll) US EPA 2001 a, 2001 b. Fmal Report: Interlaboratory VariabilJty Study of EPA ShorHerm Chronic and Acute Whole Eftluent Toxicity Test Methods, Volumes 1 and EPA-821-B*O 1-004 and EPA-821-B-0 l-005 US Environmental Proleuton Agt'.llt..:\ ( 'HJ<.:JilllJit, OH '"0 (1Q VI tH 0 -. \0 0'1 TV A I Sequoyah Nuclear Plant, OutfalllOl -UV -treated

  • October 28-November 04,2008 Statistical Analyses

,) Environmental Testing Solutions, Inc. -----------------------:-:--::---:---:--::----:-_.,..,:----,:---,::--.,---------------------* --Larval Fish Growth and Survival Test-7 Day Growth Start Date: 10/28/2008 Test ID: PpFRCR Sample ID: End Date: 1114/2008 Lab ID: ETS-Envir. Testing Sol. Sample Type: Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: Comments: UV -treated Cone-% 1 2 3 4 TVA I Sequoyah Nuclear Plant, Outfall I U 1 DMR-Discharge Monitoring Report PP-Pimephales promelas D-Control 0.4160 0.4400 0,5020 ________ ,, -- 11.3 0.5780 0.6140 0.6320 0.6220 22.6 0.5030 0.4890 0.6040 0.6060 45.2 0.5750 0.5400 0.5470 0.6390 72.6 0.5580 0.5790 0.6630 0.6880 100 0.5560 0.5570 0.5410 0.6030 Transform: Untransformed !-Tailed ,b(Jlollic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD M.:an N-Me<111 __ , D-Control 0.4618 1.0000 0.4618 0.4160 0.5020 8.778 4 0.5M2 1.0000 11.3 0.6115 1.3243 0.6115 0.5780 0.6320 3.846 4 -4.560 2.410 0.0791 0.5642 1,00()() 22.6 0.5505 1.1922 0.5505 0.4890 0.6060 I 1.480 4 -2.703 2.410 0.0791 0.5642 1.00()() 45.2 0.5753 1.2458 0.5753 0.5400 0.6390 7.842 4 -3.456 2.410 0.0791 0.564! 1.0111)() 72.6 0.6220 1.3470 0.6220 0.5580 0.6880 10.160 4 -4.880 2.410 0.0791 0.5642 I OU0\1 100 0.5643 1.2220 0.5643 0.5410 0.6030 4.759 4 -3.121 2.410 0.0791 0.5642 I lliHJO Statistic Critical Skew Kun --*------* -0.94403017 0.884 0.12865374 4 1?7R41 15.0862722 MSB MSE 0.01310714 0.00215657 % >100 >100 >100 >100 >100 >100 >100 squ!IJ! /IJ .}1),(}8<illl<i**liV IJQ !'1) !J1 c ..... Q\ Start Date: 10/28/2008 End Date: 11/4/2008 Sample Date: TVA I Sequoyah Nuclear Plant, Intake-UV-treated Test ID: Lab ID: Protocol: October 28 -November 04, 2008 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth PpFRCR Sample ID: ETS-Envir. Testing Sol. Sample Type: FWCHR-EPA-821-R-02-013 Test Species: TV A I Sequoyah Nuclear Plant, Outfall I 0 I -Intake DMR-Discharge Monitoring Report PP-Pimephales promelas Comments: UV-treated Cone-% 1 2 3 4


* D-Control 100 Cone-% D-Contro1 100 Point !COS lC10 IC15 IC20 *JC25 IC40 IC50 0.4890 0.4160 0.4400 0.5020 0.5810 0.6600 Mean N-Mean 0.4618 1.0000 0.6265 1.3568 % SD >100 >100 >100 >100 >100 >100 >100 0.5920 0.6730 Transform:

Untransformed Mean Min 0.4618 0.4160 0.6265 0.5810 95%CL(Exp) Max CV% N 0.5020 8.778 4 0.6730 7.455 4 1 1?7Q??Qil Linear Interpolation (200 Resamples) Skew -----**------. !-Tailed Isotoni,; t-Stat Critical MSD Mean

  • -----***----

.. 0.544 I I .0000 -5.328 1.943 0.0601 O.Htl I OilOll Critical 0.749 47.4672279 MSB MSE 0.05428513 0.00191229 sqn/01 /II ..'8 IJNdut,, 11v '"0 C1CI tl) Ul Ul . .e., \ ,.r;;,i Environmental Testing Solutions, Inc. Control 11.3% 22.6% 45.2% 72.6% 100% 100% Intake TV A I Sequoyah Nuclear Plant, OutfalllOl -UV-treated October 28-November 04,2008 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project numlocr: 5058 ' ' ' ' ' --

11 ***:on<J

,.,( Species: _?imcpiwies promela.l C:ient: TVA' Scquo_vJh P',lnt -LV Chemisrrv: 11.3% 22.6% 45.2% 72.6% 100% 100% Intake ' I ' I I I I I ' I I I --- Species: Pimephales promelas C'ient: TYA / Sequoyah Plant-CV-treateJ Concen-Parameter tration CONTROL 11.3% 22.6% 45.2% 72.6% 100% 100% Intake Page 57 of96 Page 6 of 6 Dare: /O* I I I I I I I I I I I I I I l l I, I .... ' I otal Residual Chlorine (Orion Electrode Methodl Orion 97-70) Matrix: Water, RL = 0.10 mgJL Meter: Accumet Model AR25 pH/Ion Meter P1ge ")':lge _)___ of __ /_ Analyst I tJ Date analyzed ._f-_-_-_._-<-l ..... o-"- Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. L b t I t d d a ora ory_ con ro s an ar Reference standard True value (TV) Measured value (MV) % RS=MV /TV X 100 number (rng/L) (mg/L) (acceptable range= 90 to 110%) :f:.A1SS5 7"'1 0.50 6 '-1'8-q Sample characteristics measurements: Sample Sample lD Sample characteristics number Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. L b l a oratory contra stan d d ar : Reference standard True value (TV) Measured value (MV) % RS =MV /TVx 100 number (rng/L) (mg/L) (acceptable range= 90 to 110%) 1-NSS5 7<1 0.50 0' Lj G 2_ qZ-,1% ( Page 58 of 96 Reviewed by Date reviewed [ fT-' tl* Cl! I I I I I I I I I I I I I I I 1.

,41! Analyst I 11/' Date analyzed 6-C!'{ L b l a oratory contro stan d d ar
Reference standard number -;J.,/IJS55

}1 measurements: Page I D P1ge __ ; _ of_/ __ Total Residual Chlorine (Orion Electrode

VIethod, Orion 97-70) Yfatrix:

Water, RL = 0 10 mg!L Meter: Accumet Model AR25 pH/Ion Y!eter Iodide reagent: JA//2_3 2! Acid reagent:

. /1/ ;2. J 0 C
, True value (TV) Measured value (MV) % RS=MV /TV X 100 (mg!L) (mg!L) (acceptable range= 90 to 110%) 0.50 o .. <::s J Sample characteristics Sample ID Sample characteristics Note. All samples were analyzed in excess ofEPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standar d : Reference standard True value (TV) Measured value (MV) % RS -MV I TV x 100 number (mg!L) (mg!L) (acceptable range= 90 to 110%) .Y/JJss*s7'f 0.50 0.'-1'6{

9'1 / (, I Page 59 of 96 Reviewed by Date reviewed __ _J I I I I I I I I I I I I I '-------,..... '"'** Analyst I LfH2, Date analyzed \\-0 l _ 08 _ '-i \.D _ 4 '/. ?age \ \ P1ge _l _ of_/ __ Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter reagent: I INIZ.3Z.I Ac1d reagent: 'T.NR-30<o Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. L b I a oratory contra stan d d ar : Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 number (mg!L) (mg!L) (acceptable range= 90 to 110%) 0.50 \01-S ;_ ... Sample characteristics Sample ID Sample characteristics orine Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. L b t l t d d a oratory con ro s an ar : Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 number (mg/L) (mg!L) (acceptable range= 90 to 110%) -a:N<:,SS 1 t:t 0.50 ().4cn qq, 4'/. Page 60 of96 Reviewed by 1-f ---:<jf6>>--=-----J Date reviewed L[ _ __J I I I I I I I I I I I I I I I I Page 61 Analvst I Date analy;ed Alkalinity (S:.\1 2320 B) Matrix: Water, RL = l.O mg CaC03/L Titrate samples to pH= 4.50 S.U. Page Page ___ ,Jf_.,----- TITmime .ceoimmptliaetteedd 1--1


1 [ H*-'>;;>::>

Titrant normality_ and multiplier determination: pH of Normality Deionized Titrant check Begin End water reference standard ml ml = 4.5 s.u. number number J* ,;:, ...... *r r....!-s:; 1 c l.' j.J,. I L b l d a oratory contro stan ard: Reference standard True value Sample number (TV) volume Begin (mg CaCOJiL) {ml) ml ((en-100 100 1..1.1 Total ml (E) !.)..I End ml .Jt.v Normality (N) of H2S04 pH Factor or Multiplier = (5 ml Na2C03 x 0.05)/E = (N x 50000)/ 100 ml sample = 0.25/E (acceptable range= 0.0180-0.0220) (: .(> ; Total Multiplier ml C( \) IC ::) Multiplier Alkalinity (MV) (mg CaC03/L) (\ r::.r l ... Alkalin;ty (mg CaCOYL) =Nx 500 j c: ::;, % RS = MV I TV x 100 (acceptable range = 90 to 110%) CI?)O{.c %RPD= {(S-D) /[(S+D)/2)} X 100 (acceptable range=+/- 10%) IC3 s D 'k atrtxspt e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaC03/L) (ml) ml ml ml :r tt c ... t K,' ,.., lCO 2-7.1t '") j()(c iC.3 liC ")>:,.\, Sample alkalinity (B) Measured spike value (MV) % R= MV /.SV x 100 (mg CaCOYL) MV =A -B (acceptable range (mg CaCOYL) = 75 to 125%) I.RC' 5o ( " l ['(' '((' S I amp*e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOYL) 1 o. iS*o8 m" ':J 1-ho 1c*t* .............. v >-rJ, h Q . .) . '*, !C t "(\ 1 i L.Je) l (/(1 .. -/" :>.' s (!. tee 10*l'l*u8 i-bO S',\ '6 :j I 2,2 I 0-13 .ut) (+ rnw::. H 1..tl :i ;j s t).tl l£* I 10* 1'5 ,(..-6 'SSh) HvD lS -l .3*'+ j -..., l 63tf' JOOCJJ'-\ j6 ) 2r.: 1 ,g \ ,. ' '-':J I /*y,* e'-L.J I JtJOe-!)5

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  • =,=-* =-, ===== Alkalinity (SM 2320 B) !vlatrix:

\Vater, RL = 1 0 mg CaC03. L Titrate samples to pH= 4.50 S.U. Page __ _ Page ___ of Tim' initiotod i Time completed ........__

  • Titrant normality and multiplier determination:

pH of Normality Normality (N) of H2S04 pH Factor or Multiplier Deionize.Q. Titrant check Be£ in End Total = (5 ml Na2C03 x 0.05)/E = (Nx 50000)/ 100 ml sample water reference standard ml ml ml fl 1 '\IF. = Nx 500 = 4.5 s.u. number number (E) (acceptable range-0.0180. *L L b a oratory contro I standard: Reference standard True value Sample Alkalinity (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaCOJIL) (ml) ml ml ml = 90 to 110%) \.( L'1-100 100 3-3S 1-f* j\:,; el--l cr7 e*-Lt:-Alkalinity %RPD = Begin End Tota*l Multiplier (mg CaCOJIL) {(S-D) /[(S+D)/21} x 100 ml ml ml (acceptable range=+/- 10%) Duplicate (B) M 'k atriX spt e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaCOJiL) (ml) . ml ml ml lccc 5 f-i i) _[' '1? [t)JO 2-; (' c Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOYL) = 75 to 125%) '5i$*C c.*I--F.-;4: S I amp,e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID l_ml) ml ml ml Multiplier (mg CaCOyL) (' -1 ) p.. qJr-., ') ,_, ' DA ,j iJ ">lfll K J_ !5t;J-';7 'fJ .. " -!.:'I t*-l

  • lD3 -bC'C> (){I OIL-\. G \

I jC() {., .j 5 '1 l -3S 1'1 R 101fc:,.o-z.. I l I cl 9, ().\/ I r .r;, jC{ /. '*I ') ,!, 3 J_ jCi 3 2L p , *. {.!.]. 1 'lt.t nt /1'"114. 01- .c *. j .. t: ---0 I .:=)CJ l . "'# -*. \ OtJ ()I b. (:)"2-I .j I *'-t\,ct_*, z l c-. c IJ l i I I (1f l OlJol&.<rl .It -::r ..J __ il j \j (. l 1 ( -' ! 2: I --.t.tf"';_..:' IJi ol*o*'-'* 03 fiokbuno N\1 I !O<> I. :2 : ,.:):) 't t.-j-., \ ;4-Lj. l liD. .-l,. z. L-i-} 'l .J_. .q.<, Reviewed by: Date reviewed: [ /0. 10* v0 I I I I I I I I I I I I I I I I I I I Page 63 o \ -- -est1ng Analyst 1 ;; ( Date analyzed ====== A.lkalinity (SM 2320 B) :Vfatrix: Water, RL = 1.0 mg CaCO,iL Titrate samples to pH = 4.50 S. U. *..)...., Page ---'---?;1g:: ----'--of--"--- Time initiated I ....___ --;... Ttrne completed L_ ______ -' Titrant normality and multiplier determination: pH of -Normality Normality (N) of H2S04 pH Factor or Multiplier Deionized Titrant c "" End Total = (5 ml Na2C03 x 0.05)/E = (N x 50000)/ I 00 ml sample water reference standard ml lii1--= 0.25/E =Nx 500 4.5 s.u. number number (acceprau*< ,,., 0.0180-0.0220) -Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaC03/L) (ml) ml ml ml = 90 to 110%) .;: N <Js \!.J '5 t 100 100 ")q I; cj.\ lt.3 q;:s,:;-lc Alkalinity %RPD= End Total Multiplier (mg CaCOJIL) {(S-D) /((S+D)/21} x 100 (acceptable range=+/- 10%) Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaC03/L) (ml) ml ml ml "-\SS 50 )00 ,5 J'S l.* I o \v,3 Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaC03/L) = 75 to 125%) lit-l 66'). s amp*e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOJIL) o& 1 O\'-\. c'-\ h, . \e-hhvw 1\i'J? \ /OC J..1S \i." -:I '*. }'\).; 'll 04 I 0 1\o. ()\.\ i ) l \ ;{\{, + E)0 l (,,_-I <t-0 g 101 &. (>L\ J 3 J '? .l-.t-¥1 .. , ___ --- -* ---,/}-f, --, . .,. _ ..... .-..----""' .. o.e:: Reviewed by: Date reviewed: IIU..jqll0 ' ,, . *. o'-!:: -\ ... -* Env1ronmer,ta1 -e.mnq Solutions, Analyst p sc f-----""'-='-----i Date analyzed 1 1-1:: *2-_ \) S '---------' Alkalinity (SM 2320 B) .'vfatrix: Water, RL = LO mg CaC03/L Titrate samples to pH= 4.50 S.U. ___ of Time initiated Time completed L. ---Lll __ ___, Titrant normality and multiplier determination: pH of Normality Deionized Titrant check water reference standard = 4.5 s.u. number number J:'"IVSS

)i.e'i L b t l d d a ora ory contro stan ar : Reference standard True value number (TV) (mg CaCO}IL)

.J..NSs vo'+-100 Sample ID M, t. 'k a Tl.X spt e recovery: Reference standard Spike value number (SV) CaCOJiL) $0 Begin ml ":) 0 Sample volume (ml) 100 Sample volume Sample volume (ml) IDO Sample alkalinity (B) (mg CaCO}IL) 59 ample measurements: s l End ml II. '1-Begin ml Begin ml Normality (N) of H2S04 pH Factor or Multiplier Total = (5 ml Na1C03 x 0.05)/E = (N x 50000)/100 ml sample ml = 0.25/E =Nx 500 (E) (acceptable range= 0.0180 -0.0220) )j.'f tc.C\ b .o ;;l. () '1--lo 3 Alkalinity (MY) % RS=MV/TVx 100 End Total Multiplier (mg CaCOJIL) (acceptable range ml ml = 90 to 110%) '1-3 10.3 qev Alkalinity %RPD = End Total Multiplier (mg CaCOJiL) {(S-D) /((S+D)/21} x 100 (acceptable range=+/- 10%) Spike alkalinity (A) End Total Multiplier (mgCaCOy'L) ml ml lio-1 I D.;> jl)._3 Ito Measured spike value (MY) % R =MY I SV x 100 MV=A-B (acceptable range (mg CaCO}IL) = 75 to 125%) 51 ID'L e-zc Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC03/L) 1 0 * ;z.t).o80 \1z.D fDO .c ,t::; 'l lv. 3 (pi I D-25.LJ0A I I JJ.\) h'lS ::>. laO I0 .. 4 33.1 se . I ltl ITS.._ /Do\342 351, I.Cj .Jo 633"1'3 \ If 1001343 .IL1 J.Ci 2-o 633-:N j 41-100844 J-tD. I d.\..0 .;?t-- rn 11s vv I 4-f,_, Li<o.O s.q V\ to* 'ZS*of A I z._ c::-0 5.-1-5.7-#:)q lr Jp-1.S-()l f> j 3 .. 5.1-tiS -ltD Reviewed by: Date reviewed: j I\ .IJ 5./T& r:-* ;,* I I I I I I I I I I I I I I I I I I I Page 65 '\-**- .---*---------

rn.moniT'ental-estrnq

.:i:.1ut*ons.

nc Analyst
  • . Date analyzed -.'$' __ _, Alkalinity (SM 2320 B) .\.latrix:

Water, RL = 1.0 mg CaC03/L Titrate samples to pH= 4.50 S.U. ?age ).., Page -------"'-'----0 f __ _ T!Tmime ceolm:mp*tJ 1*aetteedd .. -=-----t . ----.. ---.. .*. Titrant norma/it]'_ and multiplier determination: pHof------- Deionized Titrant chec .... water reference standard = 4.5 s.u. number number L b t t I t d d a ora o_ry con ro s an ar : Reference standard True value number (TV) (mg CaCOJIL) ..Jl'-.!'_;s l{C1-100 D. in = ml Sample volume (ml) 100 Sample volume Normality (N) ofH2S04 pH Factor or Multiplier End Total = (5 ml Na2C03 x 0.05)1E = (N x 50000)1 100 ml sample ml mr =Nx 500 (E) so-0.0220) - Alkalinity (MV) % RS = MV I TV x 100 Begin End Total Multiplier (rng CaCOJiL) (acceptable range ml ml ml = 90 to 110%) 1\<) Cj_Cj 1\.\3 ll:2. Alkalinity %RPD= End Total Multiplier (rng CaCOJIL) {(S-D) /((S+D)/2]} X 100 (acceptable range=+/- 10%) .\.,-Duplicate (B) ,;n, M, 'k atnx sp1 e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (rng CaC01/L) (rng CaCOJiL) Jml) ml ml ml 2C :::.s t* -:r \CD r.:n.1 31.( lc '+ 1<>.3 llO Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJiL) MV= A -B (acceptable range (mg CaCOJIL) = 75 to 125%) 5-2 ICJ..I..C:l:c S I amp*e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml rnl Multiplier (mg CaC01/L) 0&10"'2.1*0\ 5D 37'5' t-ft 3.2. '2l )0 .3 l._;.(t 0&\02'\*0\ z.. L£,7-*-P q z :t_\ o a' o C> \ 3 t)-60 '-+! 1, ;s:;, tz) lt* \ lo& so'l-1*01-11A9;.,J I f.JI ' ()_(\ '3 7_ 3 '2--*.) L*l

  • oct JC"Z.'\.OL J L 3L v..?> lz) L:*'f OK 10 '01* 0'2-J 3 J:_J;1-.

qq \ ,, 1 ) *-r if* _n.E-Io"'2..1.ot lvA.-SL.t'J IDl UJ \ 'iC. . I '(:, I II 1:1 lr-+ nf'ID"2.'\*01 I z_ 12 .c.' ifc.L 3_.-. (;<) l lc rfO&"I0-31*0\ I 3 llr-1 2i:,.\ )C, i 1..)--Reviewed by: Date reviewed: [ j(-0.3 *LV , *.. _, I I I I I I I I I I I ' I I I I I I I I Page 66 f A'. ---- Solutions. 'nc Alkalinity lSM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Titrate samples to pH= 4.50 S.U. P age __ _ ?1ge ___ of ___ _ 1 ura"' "u rltinlier determination: -** pH of Normality --. ...__Normality (N) of H2S04 pH Factor or Multiplier Deionized Titrant check Begin End Total = (N x 50000)1 100 ml sample water reference standard ml ml ml = 0.25/E **--------=Nx.SOO =4.5 s.u. number number (E) (acceptable range= 0.0180-0.0220) ------t** L b a orato_ry_ contro l stan d d ar : Reference standard True value Sample Alkalinity (MY) % RS = MY I TV x 100 number (TV) volume Begin End Total (mg CaC03/L) (acceptable range (mg CaC03/L) (ml) ml ml ml = 90 to 110%) _l r--./.::. ':::> i.e C}-100 100 :-1 \i l c:)Cl e:; Cp+ I;:; .,; Cj-*j CIT;.!(,_ Sample Alkalir.ity %RPD = volume Begin End Total Multiplier (mg CaCOJiL) {(S-D) /[(S+D)/21} x 100 ml ml ml ml (acceptable range=+/- 10%) D M atnx spt e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume egm Total Multiplier (mg CaCOJIL) (mg CaCOJIL) (ml) ...... r----.@1 ml ml ----..... '-... Sample alkalinity (B) Measured spike value (MY) R= MY I SV x 100 " (mg CaC03/L) MV=A-B (1K$able range (mg CaC03/L) =7 -,.-* s amp. e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOJIL) 0'- tJOv ;Nit 50 l ? --:), I :7' jv.2 l.: l..e 02 J 2 I -:u_, 0 ; "1 t ; \ I lr '\ oRIOo\*01-j 3 ..J ::2_( 0 39 cl jc ('1 I) J_ s*c *--....-------


c--t..J./ ? ---------------__ ., ll.---_____ ....------/ --------


Q(\_ > Reviewed by: J Date reviewed:

I LD3. t,t) J I I I I Page 67 Page Page of_,.__* __ Total Hardness (S"Yl 2340 C) RL = cr,g CaCO, '-Analyst 1 ;l;') L r--'--'-"--=-------i Date analyzed i ( 1 q C.::; Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml m1 = 0.2/E = (N x 50000)/ 50 ml sample number number (E) (acceptable range= 0.0180 -0.0220) =Nx 1000 .:I-NS:; 5\..-v D.O (1 1 CJ1 e .C;>. t> Lc ,{u -L b d a oratory contro stan ard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaC03/L) (ml) ml ml ml = 90 to 110%) ]::l'JSS 511 40 50 l"l.L.' c{l ':) I <=j .. 3-:? 0 qk:t'?c Sample Hardness %RPD= volume End Total Multiplier (mg CaCOJiL) . {(S-D) /[(S+D)/2]) x 100 zo.2-s Cj") -1 Duplicate (B) i.. ...1... D .;(t.;, .::L 3C <j s.rl C\5 jccC"lc Matrix spike recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (mg CaCOJIL) (ml) ml ml ml 5'11 .t-fO 5o 3;1./ 5 2.C .*2--1 ::;o Sample hardness (B) Measured spike value (MV) *o;. R=MV I SV x 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaCO}IL) = 75 to 125%) clS' 35 "l .. ,;:7.c '6 I Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mf! CaCO}IL) TV=ND Blank NO (should be= 0 mg CaC03/L) 50 ?;:<} 0. ;::;, z_;;,,-z_ 10-1'5.'-15

s. Hz_D .3ll 31.3 10--16-a?

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  • lq I I I I I I I Page 6 Analyst t--/

(=-*-----! Date analyzed "-1-"-"!t,_* _ ___, Total Hardness {S.:YI 1340 0 R..L = J.') mg CaCO,iL Page P3.ge J-of ) .. Time initiated Time completed .__ ______ _J Titrant normality and multiplier determination: Titram l*v " D. J<', " Total Normality (N) of EDT A pH Factor or Multiplier " "' reference standard ml ml ml = (Nx 50000)/ SO ml sample number number (E) (acceptable range= 0.0180- --. _3.Nx 1000 L b l d d a oratory contro stan ar : Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJ/L) (acceptable range (mg CaCOJIL) (ml) ml ml ml = 90 to 110%) s1 \ 40 50 nr:s JY v ..,.?.l IGS\-l.o . _j.-Hardness %RPD= End Total Multiplier (mg CaC03/L) {(S *D) /((S+D)/2!) x 100 atrrx sp1 e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (ihg, CaCOJIL) (mg CaCOJIL) (ml) ml ml ml J::NSS 511 eo J.f di.O l.,;;\( (2.) 2_o .*?.-,;( '1 0 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaC01/L) MV=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) 1qo <()a l S I amp, e measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC01/L) TV-ND Blank .... v ulg c..aLUJ/L) 0& 10 \t. 01 "1 )r,r;>, 3 tt:; .305 55 12) 2.0.2 o&HH'-l. 03 00 I I Ko *) 3S.<, I Y.;t 0410it.,c:T'll I 7. I :3$ .\c -'+t .It-;rc .Lfc otJo'Q .ol-j 3 I 4;: -, dl Lj,;J.. 0£. IOI'J. bJ\d J .'k*1)

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\ 2 3;;l, I I .Cl 3(.(' b& 1 cll2. j 3 -.. (' '35 11 l. ct .--.JAJ 1-.7{> -e.--I Sl nf Qt;; Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: J .. t*L-Date reviewed / /6-jq.t.,() I I I I I I I I I I I I I I I I I I I Page 69 --- 5o1Utl01'1 ,, !1C. Analvst i hD \._ Date analy;ed r---i---l""_)--=---'-V-_-----l _ ll-L Total Hardness (S:VI .2340 C) RL =: 0 mg CaCO,,L Page )'1 Page ___ of ___ _(__--__ Time initiated Time completed Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml = 0.2/E = (Nx 50000)1 50 ml sample number number (E) (acceptable range= 0.0180-0.0220) =Nx 1000 OD Q .\J C}.0 D-02C<3 w.e I d d a oratory contro stan ar : Reference standard True value Sample Hardness (MY) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJJ'L) (acceptable range (mg CaCO:VL) (ml) ml ml ml = 90 to 110%) \ J.}SS *51-1 40 50 q_c,., I 1.'-1' 1.0 :lo.e Li2 10..J .o Hardness %RPD= Sample volume End Total Multiplier (rng CaCOJJ'L) {(S-D) I[(S+D)/2]} x 100 'k atnx spl e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJJ'L) (mg CaCO:VL) (rnl) ml ml ml IJO so l(t).5 05 10.6 1-+0 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaCOJJ'L) = 75 to 125%) ON Lfw 115.-o'l S I ampje measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC03/L) TV=ND Blank 9D 6 rJ) (should be= 0 mg CaC03/L) 5b 0,0 fJ cl li.O I D 2<1 -btJf) m tt-s 1-1:2 o M.o a'-Uo <--l.u l qlP to .fJl;.ue a i 62-2-4.v f q(o {LJJ;5,,L(J@ J 32.1 8lc.5' LL3 m 10-mHs v.J I l .. \_1 o_£ 10-"2'5-c8 A 2 i.\o.<o Y.G '()* ""l'S-o& & 3 0.0 4A qz f\ 4 L--\.Lt fl) lp '-\-.2.-PI-t 'n&1o21. o \ 10 I I ln.f.J 2.2-{2.) iiZ .r£Mn01.'\. o \ l i 10.6 1'2--:6 z_Q i '-* B-6 Note: If >!Sml oftitrant is used, sample must be diluted. Reviewed by: Date reviewed I 1/ u0 I I I I I I I I I I I I I I I I Page 70 A:1alyst i 1----+-+='------1 Date analyzed '------'--'----=---' Total Hardness 23-10 C) Rl = l.O mg L P:ige ___ of_;_, _ Time initiated !,--,-Time completed Titrant normality and multiplier determination: Titi=am-k Begin End Total Normality (N) of EDT A pH Factor or Multiplier reference standard --**-mr-uti = 0.2/E = (Nx 50000)/ SO ml sample number number (E) range---;-o:-OT81r=-u:-onor---


.. 4-Wx 1000 L b I d d a oratory contra stan ar : Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (rng CaC03/L) (acceptable range (mg CaCOJJL)

(ml) ml ml ml = 90 to 110%) 611 40 50 JJeJ t4.*J ')_Q ;Za.e 4-z_ IC6.b/. recision: Sample Hardness %RPD= Sample Sample ID volume Begin End Total Multiplier (mg CaCOJIL) {(S-D) /((S+D)/2]} X 100 number ml) ml ml ml H0 J(;,(tJ r.e t) zo,e; s /_q -i J D /G_1,(.p It]') atrLX sp1 e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (mg CaCOJIL) (ml) ml ml ml i Srt::;-:}1 1 r J._) 0D fr..d-' VJ5 :;, 1 .:J. t2) JfJ.A ;vo Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) ')c1 6i 1D 1.2 I S I amp e measurements: Sample volume Begin End Total Hardness Sample number Sample ID -(ml) ml ml ml Multiolier (mg CaC03/L) TV=NB-Blank (should be 0 mg CaC03/L) cg *c-rt. 01--IJA i>Jr I 61) l*?n 5' dtf. t./ 3 .c1 8 fjl Cf IQ2q .oz... I 2 I , U* YO 3_5 too\. o-z... j '3 J Jt).'-f 3"'7 3tl .. I.<..-oi ac2'1-o\

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J0.z.. Lll-1 3q l'l& 10"2 (\ .o 1-I J_ I 'i2.1 1'-fL.l"i 3q .;I \.) () 1-J 3 '-1 3.<1 U.'\ {_., 3,1 -Note: If >15m! of titrant is used, sample must be diluted. Reviewed by: I Date reviewed I Page 71 of96 Sequoyah Nuclear Plant Biomonitoring October 28-November 4, 2008 AppendixD Reference Toxicant Test and Control Chart Pimephales promelas Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc. \ Environmental Testing Solution;: Inc. ,-.. -u -eJ) .._, II'J N u ;;..., eo: "'0 I t--1.2 .. - 1.0 0.8 0.6 0.4 1.2 1.0 0.8 0.6 0.4 1.2 1.0 0.8 0.6 0.4 USEP A Control Limits (+/- 2 Standard Deviations) USEPA Warning and Control Limits (75th and 90th Percentile CVs) -*-*-**-*- ---*--**-**-**-*-


*-**-*-*--* -*---Laboratory Warning and Control Limits (lOth and 25th Percentile CVs) Test date 7-day IC25 = 25% inhibition concentration.

An estimation ofthe concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC25) Warning Limits (mean IC25 +/- S AIO or S A75) Page 72. of Limits (mean rc25 +/- s A25' s A90' or 2 Standard Deviations) ""d (JQ ('D -...l w Environmental Testing Solutions, Inc. Test number Test date I 2 3 4 5 7 9 10 ll 12 13 14 15 16 17 18 19 20 01-15-08 01-29-08 02-05-08 02-12-08 02-19-08 03-04-08 03-11-08 04-15-08 04-15-08 05-06-08 05-20-08 06-03-08 06-10-08 08-05-08 08-12-08 09-09-08 09-11-08 10-07-08 10-14-08 10-28-08 7-day IC,5 (&'LKCI) 0.64 0.64 0.65 0.64 0.64 0.71 0.69 0.59 0.60 0.62 0.66 0 64 0 76 0.69 0.76 0.79 0.59 0.57 0.63 0.79 CT (g/LKCI) 0.64 0.64 0.64 0.64 0.65 0.66 0.65 0.64 0.64 0.64 0.64 0.65 0.65 0.66 0.67 0.66 0.66 0.66 0.66 s 0.00 O.DI 0.01 0.01 0.03 0.03 0.04 0 04 0.04 0.03 0.03 0.04 0.04 0.05 0.06 0.06 0.06 0.06 0.07 State and USEPA Control Limits CT-2S CT+ 2S 0.64 0.62 0.63 0.63 0.59 0.60 0.58 0.57 0 57 0.57 0.58 0.56 0.57 0.56 0.55 0.54 0.54 0.54 0.53 0.64 0.66 0.66 0.66 0.71 072 0.72 0.72 0.71 0.71 0.71 0 74 0.74 0.76 0.79 0.78 0.78 0.78 0.80 Pimephales promelas Chronic Reference Toxicant Control Chart SA.IO 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 Laboratory Warning Limits CT-SA.Io CT +SA. In 0.56 0.57 0.57 0.56 0.57 0.58 0.57 0.57 0.56 0.57 0.57 0.57 0.58 0.58 0.59 0.58 0.58 0.58 0.58 0.71 'Q72 Q72 0.72 o.n on 0.72 Q72 0.72 0.72 Qn Qn SA., 0.13 0.13 0.13 0.13 0.14 0.14 0.14 0.14 0.13 0.14 0.13 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 Laboratory Control Limits CT-SA.zs CT + SA.2S 0.50 0.51 0.51 0.51 0.52 0.52 0.51 0.51 0.51 0.51 0.51 0.51 0.52 0.52 0.53 0.52 0.52 0.52 0.53 077 0.78 0.78 0.78 0.79 0.80 0.79 0.78 0.78 0.78 0.78 0.79 0.79 0.80 0.81 0.80 0.80 0.80 0.80 SA.7S 0.24 0.24 0.24 0.24 0.25 0.25 0.25 0.24 0.24 0.24 0.24 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 (IS EPA Warning Limits CT-SA.15 CT + SA.75 0.39 0.40 0.40 0.40 0 40 0 41 040 0.40 0.40 0.40 0.40 0.40 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.88 0.89 0 89 0.88 0.90 0.91 0.90 0.89 0 89 0.89 0.90 0.90 0.91 0 92 0.92 0.91 0 91 0.92 Nore: 7-d JC15 = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. CT = Central tendency (mean IC25). S = Standard deviation of the TC21 values. Laboratory Control and Warning Limits SA'JU 0 29 0 'LV 0.2Y 0.29 u 0 30 0.29 0 29 0 0.29 0.:>9 0 2Y lUll 0.30 0.30 0.30 0 30 0.30 liS EPA Control J.iulil3 l

  • r-sA.'J!I c..* r s .

[))) I) J:> (J \") (1 ):-, u jb ll _l6 () 36 () 3.--i () 35 0 35 II.\) I) .l6 0.36 lUo 03'/ 017 0 .. \6 0.36 l) ")'/ lJ V:! 11-'!3 t)l)-i n l)J iJ 95 lJ 9) 11'1*1 0 I.J) I) 93 II 0.113 O.VI \101 1106 1197 II% U.'Jb 0.95 0 911 cv 0 00 0 02 0 0 I 0.01 0.04 ll 0) (J 06 0 Oo 0 06 0 05 0 0) 0 117 0.07 (J (18 0 09 0.09 o.M 0.10 Laboratory control and warning limits were established usmg the standard deviation of the IC25 values corresponding to the lOth and 25th percentile CYs. These ranges are more slringentthJn ihc C<lntrol and WUIIilllg lll>liiS recommended by USEPA for the test method and endpoint. SA.IO = Standard deviation corresponding to the 10'h percentile CY. (SA to= 0.12) s._25 = Standard deviation corresponding to the 25"' percentile CV. (S;. 25 = 0.21) USEPA Control and Warning Limits SA.JS = Standard deviation corresponding to the 75"' percentile CV. ( SA.JS = 0.38) SA.9o = Standard deviation corresponding to the 901h percentile CY. (SA 90 = 0.45) CV = Coefficient of variation of the IC25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH Orgam.\ms obtamedfrom

Aquatox, lnc. .11*1, !ll-21::*DH Precision of Endpoint

}leasurements Pimephales promelas Chronic Reference Toxicant Data \ "" Environmental Testing Solutions, Inc. Test number Test date Control Control Mean CT cv CT Survival Growth (%) (mg/larvae) for Control Growth (%) for Control (mg/larvae) Growth CV (%) 01-15-08 100 0.842 10.5 2 01-29-08 97.5 0.728 0.785 5.4 8.0 3 02-05-08 100 0.818 0.796 7.2 7.7 4 02-12-08 100 0.709 0.774 2.4 6.4 5 02-19-08 100 0.833 0.786 6.1 6.3 6 03-04-08 100 0.731 0.777 6.0 6.3 7 03-11-08 97.5 0.695 0.765 5.1 6.1 8 04-15-08 100 0.999 0.794 9.0 6.5 9 04-15-08 100 0.898 0.806 9.1 6.8 10 05-06-08 100 0.857 0.811 4.4 6.5 11 05-20-08 100 0.844 0.814 19.2 7.7 12 06-03-08 100 0.918 0.823 6.1 7.5 13 06-10-08 97.5 0.724 0.815 10.9 7.8 14 08-05-08 100 0.854 0.818 15.3 8.3 15 08-12-08 100 0.674 0.808 5.3 8.1 16 09-09-08 100 0.710 0.802 11.1 8.3 17 09-11-08 100 0.824 0.803 11.1 8.5 18 10-07-08 100 0.788 0.802 1.8 8.1 19 10-14-08 100 0.740 0.799 3.2 7.9 20 10-28-08 100 0.586 0.788 6.5 7.8 Note: CV = Coefficient of variation for control growth. Lower CV bound determined by USEPA (IO'h percentile)= 3.5%. Upper CV bound determined by USEPA (90th percentile)= 20% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference MSD PMSD CT (%) forPMSD (%) 0.14 17.0 . 0.07 9.0 13.0 0.07 9.1 11.7 0.05 6.8 10.5 0.13 15.5 11.5 0.07 9.0 11.1 0.07" 10.5 11.0 0.11 11.1 11.0 0.08 9.1 10.8 0.16 18.2 ll.5 0.16 18.8 12.2 0.09 9.4 12.0 0.11 15.7 12.2 0.13 15.0 12.4 0.07 9.8 12.3 0.12 17.3 12.6 0.11 12.9 12.6 0.10 12.9 12.6 0.05 7.2 12.3 0.10 17.8 12.6 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10'" percentile)

12%. Upper PMSD bound determined by USEPA (901h percentile)

30%. CT = Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200la, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effiuent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtained from Aqua/ox, Inc. Page 74 of96 atoxl0-28-08 ..:: ..... 0 1-v -0 1-..... = 0 u -0 '-' = 0 *.;: *: ell >-.... 0 -= "' s Q) 0 u -0 '-' lf.J. A.. Pimephales pro me las Chronic Reference Toxicant Control Chart ,..,.. Environmental Testing Solutions, inc. Precision of Endpoint 1\tleasurements Organism Source: Aquatox. Inc. ,..-._ Q) > 1---.... ..... *-= *--.... bll s ..._, ..:: -0 1-0 -0 1--= 0 u 1-.8 1.25 .-1.00 -----0.75 ... .. * .............. -* ............ . 0.50 0.25 **-* -**-**---* USEPA Acceptance Criteria(> 0.25 mg per surviving larvae) 30 Kentucky Acceptance Limit(< 30.0%) 20 **-. .. . **-10 0 30 US EPA Upper PMSD Bolilld (90th percentile < 30.0%) 20 --------- .... . . . . . . . . . . . . . . . . . . . . . . . . -. . . . . . . . . **-...... . ......__ ......__ 10 Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Page 75 of 96:ontrol Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) I I I I I I I I I I I I I Ch!oridc Ch;-on:t.: Refuencc To:xican: .;;,t (EP.-\-821-R-02-013 .\tlethod l!JU*),,)\ Species: Pimepha!es promefas PpKCICR Test I C.. '2-Dilutionpreparation information: Comments: KCI CHM number: C.l-\H Stock preparation: 50 g KCJ/L Dissolve 50 g KCl in 1-L Deionized water Dilution prep (mgfL) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Test information: Organism age: ?.S aLb Randomizing template: UI"J Date and times organisms IC* 21*0A 1\oOO Incubator number and ob were born between: shelf location: Organism source: PP lO*t1*Gf Artemia lot number: Transfer bowl information: pH= SU Temperature= oc Total drying time: '*"' 1.'-IA Date I Time in: llo '1.0 Average transfer volume: 0. Date I Time out: ll*t6*o& "2.b Oven tem_E_erature: bo'C Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding time Test initiation, MHS batch used Analyst 0 2 3 4 5 6 7 Control information: Acceptance critena Summary of test endpoints: %Mortality:

07.

7-day LC50 s,,,t> Average weight per initial larvae: c.Sib NOEC Average weight per surviving larvae: O*S'Io ;::>: 0.25 mg/larvae LOEC qoo ChV 8'2.\. (&. IC2s '1U .. 8' I I I I I I I I I I I I I I Species: rmepha!:c: promelas n row l a a Survival a d G tl D t Day Control 450 mg KCI/L 600 mg KCI/L A B c D E F G H r J K _!:_ 0 ID Ia IO /0 /0 IO IO lb Jo IO 10 IO 1 10 {0 10 IO tc IO 10 /0 to IO /0 10 2 lO /0 10 10 JO 10 10 to I(J 10 10 10 3 /0 10 /D /D ID 10 IO 10 /tJ 10 /0 q'rA. 4 to /\) lb /0 f(j ID /0 10 /0 10 5 to JO JO 10 J 0 /0 IO /0 10 /0 B'J.. <\ 6 ID 10 /Q q*c.\ IC /0 g 'i 10 /D 10 /0 7 /{) /D It> ID to /D q I 0 IO 10 g t:t\l..'r A= Pan weight (mg) Tray color code:: Analyst: t .e-0 ll{:l'C 1'-\.\ft. \3.tl3 3.'03 \'\ .a1 \3.01.. 13.SG 1'-t. '-l'i !'l. 01 B =Pan+ Larvae weight (mg) :;:.:;:z.J z..z; .* \C' w' 20.bD 1S.lc l i." I, ... c b 'S.lvl -Lu!S 1 Analyst: 'Z.t.t-\ l' jl j r:, \J ""i C =Larvae weight (mg) =A-B C).lc l "S.13 ()Vi s*:;-z. 5 ll S .. "lb ') s L.Jl L1.'L) Weight per initial number ,*1*.\J IP <;'1'2 lce\.;1 lc:,,(' ....... ,t.) .J.i;'"cl of larvae (mg) 1"-\3 i/i:i '\ !1),\:;j.l. \,.i*" 11.-* . 0) <571 ic.S78 1.)<1 l' ' ) '-'* ,. = C I Initial number of larvae \;'. Average Percent weight per reduction o.S'-\\ lo .I 7o.l initial from control o.SR!o O.Siol number of (%) larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Calculations and data reviewed: Comments: I I I I I I I I I I I I I I I I I I Spt:(ies: Pimephalt!-,- prameias Survival and Growth Data Day 750 KCI/L 900 mo KCI/L 1050 m KCI/L M N 0 p Q R s T u v w X 0 JD /0 /t) /{) /{) /(J to ID It; ID /0 IO 1 q'c4. '\,cA. *cl lJ.. 4J.: 1,10\ IO /0 &' ., (Q s '-\ 2 &'J.. JD /D q [! .., ,cl l (., L.\ 'S '-\ 3 /C 10 & q & .., ,tA Ia '-\ 'S $ 4 /c 8" ,J. 4..J. /0 & ., 5 3 5 tO "l,il )fA 3l.IA leA 10 ' .f ...t 5 5 2-,_ 6 to 10 1 & '-\ lo s s 1... 1.'" 4 1 'c.\ 7 "'td i.( JJs \ s"" f() .., t..\ 'S s "2.. 3 A= Pan weight Tray color code:: .QYe.ei'J Analyst: tUl'L \'\. '\ '0 13.'\(p

13. te't l3.':>lj

!t.l..t3 lt.f.S3 t'f .00 {Ll-t'-f f'-{.S9. B =Pan + Larvae weight (mg) il.l.o\ 2..t2') i0* l M 1\""' 'i,C::i

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Analyst: ,.._ ... )! 1---ti I *.J. ' .2 1 . .-* .} \J, \p I 1..* C =Larvae weight (mg) =A-B Y.lc\ fn L.! '"\.1 \ LCc ('¢ as-! I -,,-* 0 ! 1 f.S'-1 c \.1(* l --..cc c-_>l I a.t.S'1 Weight per initial number ,,*...\'0\ . )-l '\\ 7l,:-t .-7_ 3iJ c.\':)'-1 ,.._*-ztt: \::.6"1 ... c \ c (;'1"1 of larvae (mg) V; ... '-\ \.).\ i \.i* v* ,\ .h.* \..* ' = C I Initial number of larvae \,.,* :'\c:\ lJ* Average Percent weight per reduction sa..£7. o. cf\ \ initial from control .. 0 number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed:.&__ Comments: 78 of 96 ""Q tl) -....) \C Q ..., \C C\ * * ) Environmental Testing Solutions, Inc Cunccotrl:ltiun (mg/L Juiliwl number uf KCI) tan**c A )0 Control B 10 c 10 D 10 E 10 450 F 10 G 10 H 10 I 10 600 J 10 K 10 L 10 M 10 750 N 10 0 10 p 10 Q 10 900 R 10 s 10 T 10 u 10 1050 v 10 w 10 X 10 Dunnett's MSD value: 0.1043 PMSD: 17.8 Fin:tln,umbcr of han*ae 10 10 10 10 10 10 9 10 10 10 8 9 9 10 7 7 4 5 5 5 I 2 3 1 A"' Pu weight. (mg) 14.78 14.36 14.62 13.28 13.83 14.11 14.82 13 06 13.02 13.59 14.44 14.02 12.82 14.98 13.96 14.46 14.38 13.89 14.59 13.54 14.23 14.53 14.00 14.14 MSD= PMSD= Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method l 000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses B- + Lllrv:.iC Lun'II.C weight (mg) Wcigbt I Survil'inR Mcun Ct)(ffidtnt of\ ariatiou Wcigbt f Initial number wcigbt(mg) oumbtr of larvae (mg) Surviviog number of (Mnn"C'IthlpcrlurYJ>'i.rll. nf (mg) lllJ"V.IIe(mg) nu*n'louuCllonllf) (*/G) 20.21 5.43 0.543 0.543 20.00 5.64 0.564 0.586 65 0.564 20.84 6.22 0.622 0.622 19.41 6.13 0.613 0.613 19.35 5.52 0.552 0.552 19.88 5.77 0.577 0.583 7.0 0.577 20.60 5.78 0.642 0.578 18.67 5.61 0.561 0.561 18.69 5.67 0.567 0.567 19.06 5.47 0.547 0.591 14.9 0.547 18.67 4.23 0.529 0.423 20.51 6.49 0.721 0.649 Test numbt!r: PpKC\('R

  1. 162 Test dates: Octohn :!H 1\,'ovembt:r 0--11

. Mclin sun*ival \\Cii-:1111 Juiti;tl ( 11\:nidcnt 11f Pcr(l'l\1 r4.'ilurtiou from (%) number or I <In .a: \ a 1 i ( J ron tl n l ( ) 100.0 0.586 6.5 . 97.5 0.567 !.2. 3.2 *---**----92.5 0.547 17.1 fl.7

    • -**-*------

17.61 4.79 0.532 0.479 21.25 6.27 0.627 0.622 10.1 0.627 18.67 471 0.673 0.471 82.5 0.509 I :"I.) U.l 19.05 4.59 0.656 0.459 16.38 2.00 0.500 0.200 16.57 2.68 0.536 0.503 6.4 0.268 47.5 0.239 S9.2 17.16 2.57 0.514 0.257 15.84 2.30 0.460 0 230 14.87 0.64 0.640 0.064 15.54 101 0.505 0.525 15.9 0.101 I 15.54 1.54 0.513 0.154 17.5 0.091 SJ.J I H-L:"' 14.58 0.44 0.440 0.044 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared signiflt:anlln 11 whl\k: clfhn:llltuxJcily lt.!si Lower PMSD bound detennined by US EPA (!Otl1 percentile)

12%. Upper PMSD bound detennined by USEPA (90th percentile)

30%. Lower and upper PMSD bounds were detennined from the lOth and 90th percentile, respectively, of PMSD data from EPA's WET lntorlaboratory ( 2\IU 1 "* ( ISEI' A, 201J I hl USEPA. 2001 a, 2001 b. Final Report: lnterlaboratOJy Variability Study of EPA Short-tenn Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix EPA-821-B-0 1-004 and EPA-82 1 -ll-01-005. US Environmental Prot<ctiuo 1 "'""'""* 1 lll Organisms ohtainedfrum

Aquatox, Jm:. atu-.: I0-2X

""C !'!) 00 c e .., Q\ Statistical Analyses Environmental Testing Solutlonl, Inc. Larval Fish Growth and Survival Test-7 Dax Survival Start Date: 10/2812008 Test ID: PpKCICR Sample ID: End Date: 1114/2008 Lab ID: ETS-Envir. Testing Sol. Sample Type: Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: Comments: Conc-mg/L D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 0.9000 1.0000 600 1.0000 1.0000 0.8000 0.9000 750 0.9000 1.0000 0.7000 0.7000 900 0.4000 0.5000 0.5000 0.5000 1050 0.1000 0.2000 0.3000 0.1000 Transfonn: Arcsin Sguare Root Conc-mg/L Mean N-Mean Mean D-Control 1.0000 1.0000 1.4120 450 0.9750 0.9750 1.3713 600 0.9250 0.9250 1.2951 750 0.8250 0.8250 1.1608 '900 0.4750 0.4750 0.7602 '1050 0.1750 0.1750 0.4217 Auxtliary Tests Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01) Equality of variance cannot be confinned Min 1.4120 1.2490 1.1071 0.9912 0.6847 0.3218 Hypothesis Test (!-tail, 0.05) NOEC LOEC Steel's Many-One Rank Test 7 50 900 Treatments vs 0-Control Parameter Value SE 95% Fiducial Limits Slope 8.99516854 1.26542412 6.51493721 11.4753999 Intercept -21.472864 3.69736461 -28.719699 -14.226029 TSCR Point Probits mg/L 95% Fiducial Limits ECOl 2.674 483.48978 389.172926 549.252679 EC05 3.355 575 63813 493.057452 632.425785 ECIO 3. 718 631.739647 558.367087 682.984696 EC15 3.964 672.648407 606.421898 720 344523 EC20 4.158 707.042528 646.71135 752.4602 EC25 4.326 737.947854 682.481552 782.205752 EC40 4.747 821.948177 774.797303 870.093762 EC50 5.000 877.019469 829.55876 935.129 EC60 5.253 935.780601 883.109836 1010.80758 EC75 5.674 I 042.30013 971.791044 1159.98544 EC80 5.842 1087.85981 1007.70304 1227.18709 EC85 6 036 J 143.48471 1050.48901 1311.38658 EC90 6.282 1217.53181 1106.07161 1426.66922 EC95 6.645 1336.1921 1192.70601 1618.06819 EC99 7.326 1590.85723 1371.48327 2052.73137 Organisms obtained from Aquatox, Inc. Max CV% N 1.4120 0.000 4 1.4120 5.942 4 1.4120 11.347 4 1.4120 17.825 4 0.7854 6.622 4 0.5796 29.582 4 Statistic 0.96270359 ChV TV 821.583836 Maximtun Likelihood-Probit Control Chi-SS 0 6.5280808 Rank Sum 16.00 14.00 12.00 10.00 10.00 Critical 7.81472778 REF-RefToxicant KCL-Potassium chloride PP-Pimephales promelas !-Tailed Critical 10.00 10.00 10.00 10 00 10 00 Critical 0.884 P-value Mu 0.09 2.94300923 Number Total Resp Number () 40 I 40 40 7 40 21 4lJ 33 .Jll Skew Kurt 0.12251881 0.07880166 Sigma lter 0.111 J 7079 4 at ox I U _>X OR I1Q ('!) QO ...... 0 ...., I,C 0'\

  • Statistical Analyses

" Environmental Testing Solutions, Inc. Larval Fish Growth and Survival Test-7 Day Growth Start Date: l 0/28/2008 Test JD: PpKClCR End Date: ll/4/2008 Lab ID: ETS-Envir. Testing SoL Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Comments: Conc-mg/L 1 2 3 4 D-Control 0.5430 0.5640 0.6220 0.6130 450 0.5520 0.5770 0.5780 0.5610 600 0.5670 0.5470 0.4230 0.6490 750 0.4790 0.6270 0.4710 0.4590 900 0.2000 0.2680 0.2570 0.2300 1050 0.0640 0,1010 0,1540 0.0440 Transform: Untransformed Conc-mg/L Mean N-Mean Mean D-Control 0.5855 1.0000 0.5855 450 0.5670 0.9684 0.5670 600 0.5465 0.9334 0.5465 750 0.5090 0.8693 0.5090 900 0.2388 0.4078 0.2388 1050 0.0907 0.1550 0.0907 Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.01) Bartlett's Test indicates equal variances (p = 0.04) Min 0.5430 0.5520 0.4230 0.4590 0.2000 0.0440 Hypothesis Test (1-tail, 0 05) NOEC LOEC Dunnett's Test 750 >750 Treatments vs D-Control Max CV% 0.6220 6.509 0.5780 2.236 0.6490 17.093 0.6270 15.539 0.2680 12.720 0.1540 53.254 ChY TU Sample JD: Sample Type: Test Species: N 4 4 4 4 4 4 Statistic 0.94647986 8.28880119 MSDu 0.1042998 Linear Interpolation (200 Resamples) Point SD t-Stat 0.406 0.856 1.680 MSDp 0.178138 REF-Ref To xi cant KCL-Potassium chloride PP-Pimephales promelas - .. !-Tailed lsotllllic Critical MSD Mean N-M<:UII (),5855 I .OOflO 2.290 0.1043 0.5670 O.lJ684 2.290 0.1043 0.5465 0.'!.l34 2.290 0.1043 0.5090 O.X693 0.2388 0.4()'JX 0.0907 (1.155() Critical Skew Kiirt ---------- 0.844 0.24683066 I I 11.3448668 MSB MSE F-Prob df' 0.004302 0.00414883 0.41126868

3. 12 ------------

.. ' 95% CL(ExE) Skew !COS 528.84 118.50 171.14 891.05 -0.2714


ICJO 678.20 81.73 406.86 823.15 -0.4723 IC15 756.29 53.17 473.75 798.03 -1.6249 IC20 772.53 22.83 690.60 810.16 -3.1449 IC25 788.78 13.72 744.57 822.58 -0.0595 IC40 837.53 9.24 808.29 860.09 -0.0603 ICSO 870.03 7.66 845.69 890.11 -0.0839 Organisms obtained from Aquatox, Inc. at ox I I > '*:\'

Pimep:ut!es prvmeias 1050 mg KCI!L STOCK Page 82 of96 Page 1 ;f) Species: Pimeph,ues promelas 450 mgKCl/L 1050 mg KCI/L STOCK Page 83 of96 ,-..., -u z -bJ:) --on N u ;;;... C'l: "t:S I r-Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Environmental Solutions, Inc. 1.14 f .. USEP A Control Limits (+/- 2 Standard Deviations) 1.12 1-1.10 1.08 ---1.06 1.04 1.02 2.5 USEPA Warning and Control Limits (75th and 9dh Percentile CVs) 2.0 1.5

  • 1.0 * * * * -.= * * * * * * * * *
  • 0.5 1.3 Laboratory Warning and Control Limits (1 dh and 25th Percentile CVs) 1.2 1.1 1.0 0.9 . -. . . . . . .

. . . Test date 7-day IC25 = 25% inhibition concentration. An estimation ofthe concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. ---Central Tendency (mean IC25) ---Warning Limits (mean IC25 +/- SA10 or SA75) Page.84 of Limits (mean IC25 +/- S A25, S A.90, or 2 Standard Deviations) '"d t'D 00 Ul 4\b # ;;) Environmental Testing Solutions, Inc. Test number Test date 7-day JC25 CT (g/L NaCI) (g!LNaCI) I 2 3 4 5 6 7 8 9 10 II 12 13 14 15 16 17 18 19 20 05-08-07 06-05-07 07-10-07 08-07-07 09-05-07 10-02-07 1 J-06-07 11-06-07 12-04-07 OJ 08 01-08-08 02-05-08 03-04-08 04-08-08 05-06-08 06-03-08 07-08-08 08-05-08 09-09-08 10-07-08 1.10 ].]0 1.06 1.10 1.08 106 1.06 1.07 1.07 109 1.09 1.07 1.07 107 106 1 11 1.07 109 1.10 1.09 1 10 1.08 1.09 109 108 1.08 1.08 1.08 1.08 1.08 1.08 1.08 1.08 108 108 1.08 1.08 1.08 1.08 s 0.00 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.01 0.01 0.02 0.02 0.02 0.02 0.02 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart State and USEPA Control Limits s.._,0 CT-28 CT+2S 110 104 1.05 1.05 104 1.04 104 1.04 1.05 1.05 1.05 1.05 1.05 1.05 1.05 1.05 1.05 105 1.05 1.10 1.13 ].]3 1.12 ].]2 1.12 1.11 Ill 1.11 1. 1 1 1.1 1 I. II ].]1 1.11 Ill 1.11 Ill 1.11 1.11 0.09 0.09 0.09 0.09 0 09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 Laboratory Warning Limits CT-SA.lO CT + SA.JO 1.01 1.00 1.00 1.00 1.00 1.18 I 17 1.17 1.17 1.17 1.16 1.16 1.16 1.16 1.17 ].]7 1.16 1.16 116 1.16 1 16 1J6 1.17 1.17 SA.lS 0.19 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 Laboratory Control Limits CT -S.._,s CT + S.._,s 0.91 0.90 0.90 0.90 0.90 0.90 0.89 0.89 0.90 0.90 0.90 0.89 0.89 0.89 0.89 0.89 0.90 0.90 0.90 1.28 1.27 127 1.27 1.27 1.26 1.26 1.26 1.26 126 1.26 1.26 1.26 126 1.26 1.26 1.26 1.26 1.26 SA.7S 0.49 0.49 0.49 0.49 0.49 0.49 0.48 0.48 0.49 0.49 0.49 0.49 0.48 0.48 0.49 0.48 0.49 0.49 0.49 USEPA Warning Limits CT-SA.1s CT + SA.7s 0.60 0.60 0.60 0.60 0.60 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 1.59 1.57 1.58 1.57 1.57 1.56 1.56 1.56 1.56 1.57 156 1.56 1.56 1.56 1.56 1.56 156 1.57 1.57 SA.9U 0.68 0 67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 Note: 7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC25). S = Standard deviation of the IC25 values. Laboratory Control and Warning Limits lJS],;l'.\. Coutrull.imits CT-SA.Yil ( T I sA. Oil ll-12 0.41 0 U-11 041 041 l!-11 0.<1 1 OAI () 41 0.'11 0 41 041 l) 41 () 1 0.41 0.41 041 0.41 I 78 I /':i I 76 I /6 I /'5 /) IS n h IS I /) l.h lh I '/4 I 75 I 7) I 75 I /) I '!) cv 0 00 0 02 0.02 0 02 0.02 0.02 002 0.02 0.02 (){) 1 0 OJ 0.01 () 01 0 01 0 02 0 01 0.01 0 Ul U.UI Laboratory control and warning limits were established using the standard deviation of the 1C25 values corresponding to the 1Oth and 25th percentile CVs. These ranges are more st1 ingcntthan the> cotllrul and warning limits recommended by USEPA for the test method and endpoint. 8.._10 = Standard deviation corresponding to the lOth percentile CV. (SAw= 0.08) 8.._25 = Standard deviation corresponding to the 25th percentile CV. (SA 15 = 0.17) USEPA Control and Warning Umits 8.._75 = Standard deviation corresponding to the 75th percentile CV (SA75 = 0.45) S.._90 = Standard deviation corresponding to the 90th percentile CV. (SA'"= 0 62) CV = Coefficient of variation of the IC25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effiuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Pruteciion Agency, Cincinnati, OH /II 08 Precision of Endpoint IVIeasurements Ceriodaphnia dubia Chronic Reference Toxicant Data '\ Environmental Testing Solutions, Inc. ,iJ,¥,!1' Test Test date Control Control Mean CT MSD PMSD CT number Survival Reproduction CT cv for Control Mean for Control (%) (offspring/female) Reproduction (%) Reproduction (%) forPMSD (%) (offspring/female) CV(%) 1 05-08-07 100 32.0 8.5 2.7 8.4 2 06-05-07 100 28.4 30.2 7.5 8.0 2.5 8.7 8.6 3 07-10-07 100 29.3 29.9 6.0 7.3 2.5 8.5 8.5 4 08-07-07 100 28.7 29.6 7.5 7.4 2.9 10.0 8.9 5 09-05-07 100 29.7 29.6 5.7 7.0 2.5 8.4 8.8 6 10-02-07 100 29.5 29.6 9.4 7.4 2.4 8.2 8.7 7 11-06-07 100 28.0 29.4 6.1 7.2 2.5 8.8 8.7 8 11-06-07 100 30.4 29.5 5.6 7.0 2.2 7.2 8.5 9 12-04-07 100 31.8 29.8 5.5 6.9 2.5 7.9 8.5 10 01-08-08 100 30.3 29.8 6.6 6.8 2.2 7.3 8.3 11 01-08-08 100 31.8 30.0 4.9 6.7 2.4 7.7 8.3 12 02-05-08 100 31.3 30.1 6.4 6.6 2.8 9.1 8.3 13 03-04-08 100 31.5 30.2 8.0 6.7 2.7 8.5 8.4 14 04-08-08 100 29.6 30.2 7.7 6.8 2.6 8.8 8.4 15 05-06-08 100 32.1 30.3 6.3 6.8 2.5 7.9 8.4 16 06-03-08 100 30.5 30.3 7.9 6.8 3.0 9.9 8.5 17 07-08-08 100 30.9 30.3 6.7 6.8 2.4 7.7 8.4 18 08-05-08 100 29.4 30.3 7.9 6.9 2.2 7.6 8.4 19 09-09-08 100 28.3 30.2 8.5 7.0 3.0 10.5 8.5 20 10-07-08 100 32.4 30.3 7.2 7.0 2.7 8.4 8.5 Note: CV = Coefficient of variation for control reproduction. Lower CV bound determined by USEPA (IO'h percentile)= 8.9%. Upper CV bound determined by USEPA (90'h percentile)= 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (lO'h percentile)

13%. Upper PMSD bound determined by USEPA (90'h percentile)

47%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEP A 2001 a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. 10-07-08 Page 86 of96 '\. Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint

Nleasurements

,.,. Environmental Testing Solutions, Inc. c: 35 0 *-,-.. ...... -= 30 E *********** .... 0 ... -C.. OJ) 25 c: *-... -0.. 0 20 ... ...... """ c: 0 0 _., u 15 Minimum Acceptance Criteria(> 15.0 offspring per surviving female) -c: 40 .:z = ...... 0 Col North Carolina Acceptance Limit(< 40.0%) ..... = ."E 30 --0.. Kentuck')' Acceptance Limit(< 30.0%) 0 0 20 ...... -= -Q,l = *c:: 0 s u 10 -0 u 50 . ili USEPA Upper PMSD Bound (90 percentile< 47.0%) 40 30 20 10 Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD) Page 87*of Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations) '------+ --------- irx. Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013

VIethod 1002.0) Species:

Ceriodaphnia dubia CdNaCLCR

  1. 4'0 Dilution preparation information:

NaCI CHM number* \"2..0 Stock preparation: 100 g NaCI/1 (dissolve 50 g NaCI in 500 ml deionized water) Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test orf{anism source information: Organism age: < 24-hours old Date and times organisms were born 10*04.*0&

  • s'-4'2.-ru between:

Culture board: A Replicate number: I l 2 I J J 4 l 5 I 6 J 7 I 8 l 9 I 10 Culture board cup number: \ 11.. I .?I I 5 I 8 I '\ I t \ II'-I ,, I '1.4 Transfer bowl information: pH=1.&& Daily renewal information: Day Date 0 2 3 4 5 6 7 Control information: % ofMale Adults: % Adults having 3rd Broods: % Mortality: Mean Offspring/Female: %CV: Page 88 of96 su Temperature = '1-\.'\ Test initiation and feeding, renewal and feeding, or termination time oc Acceptance criteria o7 .. s20% ltx/1. 80% (j{. s20% .32."' 15.0 offspring/female 1* 2 <40.0% Comments: Test information: Randomizina template color: Incubator number and shelf location: YWT batch: Selenastrum batch: MHSW batch used Analyst 6UJe. l.C. \ 0 "*2.1:\-<:) f SummaryoLtest endpoints: 7-day LC5o ) 140() NOEC /OOD LOEC rz.__oo ChV ICl .. .'-\ IC2s 1 o'\ Page l of 6 I I Page 2 -:f 6 Species: Ceriodaphnia dubia CONTROL Dav 1 I Young produced 0 Adult mortality \._ 2 Young produced 0 Adult mortality L 3 Young produced () Adult mortality L 4 Young produced s Adult mortality L s Young produced \ '-Adult mortality \._ 6 Young produced \\ Adult mortality c.._ 7 Young produced Total young produced .3'-\ Final Adult Mortality L-X for 3'd Broods )<.. 2 I 0 '---0 L 0 L s L L \a L .3'-)<... Cd"'aCLCR

.
8'0 --=---Survival and Reproduction Data Replicate number 3 4 5 6 7 8 9 10 0 0 0 0 0 0 0 0 L L L-L L L L 0 0 0, 0 0 0 c () L-L L L L L L '-0 0 0 0 0 0 0 0 \.._ \._ \.._ L. '---\.._ \..._ \. 1..\ '-\ s '-\ s 5 \.... \.... \.._ L-L \.._ \.......

'--'C> l!. -' t \ I'"Z.. JC 1£.. L L L. L \,_ \..._ '-\_. t4 l'-t l"'\ \lo \& 's -1\o L \..._ \_ L \..__ L '-'-28 o\ 35 32.. 3\ !10 '-'-L-'-\....-L.. L_ 7< '>"--)<._ ><-r:-><-Note. Adult mortahty (L = hve, D =dead), SB =split brood (smgle brood split between two days), CO= carry over (offspnng carried over With adult during transfer). Concentration: % Mortality: I o1. Mean Offspring/Female: J o2A 600 mg NCVL a S . I dR urvzva an d epro uctzon D at a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 a C) 0 n 0 a 0 Adult mortality '-\_ L '--'-\...... '--L '--'--2 Young produced 0 () 0 0 0 0 0 0 0 0 Adult mortality L L L. L L L L '-L-'-3 Young produced b 0 () 0 () a 0 ()_ 0 0 Adult mortality L L L L-\._ L L L '-\... 4 Young produced lo s 5 \c 1...\ s s '-'. s Adult mortality L L L L L L L L L L 5 Young produced t"-\ \"2-ID \ "'L 1 \ \2 \ "'2.. 10 Adult mortality L L. L L L L L L L.. L 6 Young produced It IS Jl..\ IS ,., It..\ llo I_(Q II. , Adult mortality L. L L L.. \...._ L. L.. L L L-7 Young produced Total young produced 3'Z. "Z'1 ..3Y ..3b 34 ..32 32 3L Final Adult Mortality l_. L \. L L L L '-L '-Note. Adult mortality (L =live, D =dead), SB =split brood (single brood split between two days). CO= carry over (offspnng carried over with adult during transfer). Concentration: % Mortality: 07 .. Mean Offspring/Female: at.." Page 89 of96 %Reduction from Control: -o.<.,7p iJ Page 3 of 6 Species: Ceriodaphnia dubia 800 mg NaCl/L Day 1 1 Young produced 0 Adult mortality L 2 Young produced 0 Adult mortality L 3 Young produced 0 Adult mortality L 4 Young produced '4 Adult mortality L-5 Young produced Adult mortality L 6 Young produced ll Adult mortality \..-7 Young produced Total young produced Final Adult Mortality L 2 I i 0 I L 0 L 0 \...._ s L IY L ,, '--..3\c. L CdNaCLCR =t: Eo Survival and Reproduction Data Replicate number 3 i 4 5 6 7 8 9 10 0 D 0 0 0 0 0 n '-L '-l-'-\.__ L L.. 0 0 0 Q_ _0_ 0 () t> \..__ L-'--'-L L L. L 0 () 0 0 D 0 0 0 L L L.. L \...._ L-L L s '-\ '-\ Ia s s s '-\.._ '-L.. L L.. L.. '-\ \ 10 \L. \'1... l"l. 12.... I\ \.._ L L.. L. L L L L.. l i l(o 1'-l 1"-\ \q ,, 1"4 L L. \..._ L.. \._ \.._ '---'--3'-\ 3o 3\ .:!,C 3'l. 31. 3() L. '-L \.. \..__ \..._ L.:_ Note: Adult mortahty (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offsprmg carried over with adult during transfer). Concentration: % Mortality: Oi Mean Offspring/Female: 3"1..:, % Reduction from Control: 1000 mg NCl/L a S . I dR urvzva an d t' D t epro uc zon a a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 a 0 0 Q D n _Q_ () () Adult mortality L l L L.. \..._ L-'--\.._ \.__ L. 2 Young produced (') 0 0 0 0 0 0 0 0 0 Adult mortality L L \..._ L L-L L L L L 3 Young produced () 0 D D 0 6 a 0 0 a Adult mortality L.. L L L L-'-L '-L... L_ 4 Young produced '-\ s -s \o '-\ s '5 4 '-l l..:, Adult mortality L L L '--'--L L L_ \..._ \..... 5 Young produced IC \'2. tO I l 1¢ l& IL... IC IQ Adult mortality L L L L L L L L L '-6 Young produced \$ 1'5 L"-\ *1 l'-\ lq llo ,-; Adult mortality \__ L L.. L L... L L L L L-7 Young produced Total young produced .30 3'2 3-b 2>2. 31.. 32 32,. 2'\ 2'\ Final Adult Mortality L '-L L \..._ L... '--L '-Note.* Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood split between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: % Mortality:

01. Mean Offspring/Female: Page 90 of96 %Reduction from Control:

J .

. I I I I I I I I Page 4 or* 6 Species

Ceriodaphnia dubia CdNaCLCR#: POO Cl/L -mg. a Survival an dR d epro uctzon D at a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 D 0 0 0 0 0 0 0 0 Adult mortality L '--L-L-L-L-"--'--L L_ 2 Young produced 0 () a 0 0 _Q_ 0 0 Q 0 Adult mortality L. L '-L '--L.. L '--L l.,_ 3 Young produced 0 0 0 C) 0 0 c 0 6 () Adult mortality \._ '--. '--\._ L... L_ \.__ l.-L \.._. 4 Young produced I.{ '4 '-\ _S_ --':l 4 4 .3 '-\ Adult mortality L '-'--\.._ \...... L '-L. L L 5 Young produced l "" '5 I D lD '-\ , lC "' Adult mortality. L L \..._ '--'-L '-\.._ L 6 Young produced ID 0 s s 8 s 0 IC 5 d Adult mortality '-'---\_ L \_ '---L \_ L \..__ 7 Young produced Total young produced 2\ 13 1.:!> L'\ t'\ 13 I \ 1.& "2.\ Final Adult Mortality \,. \._ '--'-\...... L. '--'--\.._. \. Note: Adult mortality (L =live, D =dead), SB =split brood (smgle brood spilt between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: %Mortality: ()1. Mean Offspring/Female: \1. \ % Reduction from Control: q,.,.,. 1400 mg NCl/L a S . I dR urvzva an d r D t epro uc wn a a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 a_ 0 0 0 0 Adult mortality '--l-\...._:.. L-\._ L__ L-L.. L \.... 2 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L L-L L. L L L... L \...._ 3 Young produced (') 0 0 0 0 0 D 0 0 () Adult mortality \.._ \._ L L L L L L L. l 4 Young produced y 0 _'-\ '-'-\ '-'-\ 4 .3 Adult mortality \..._. L '--L L-\_ \_ '-L. '-5 Young produced '-l '-c s \ 3 I q to '-Adult mortality L L L-'-'-\...._ L.. L \.._ \..._ 6 Young produced 0 0 I 0 0 0 0 0 0 Adult mortality \..._ \..... \...._ \_ \_ \._ L L.. '--L-7 Young produced Total young produced & :s s .& 3 I 3 g /0 10 Final Adult Mortality L L '-L. L L \._ L L L Note: Adult mortahty (L = ltve, D =dead), SB =spilt brood (smgle brood spht between two days), CO= carry over (offsprmg carried over with adult during transfer). Concentration: % Mortality:

01. Mean Offspring/Female:

U/.1 Page 91 of 96 %Reduction from Control: 1'f, 3?o .. ,, 1>4 v Verification of Ceriodaphnia Reproduction Totals Environmental Testing Solutions, Inc. Control 1000 m!! NaCUL "d (J(l ("> \C w 0 -. \C 0\ * * * '<) Environmental Testing Solutions, Inc. Concentration (mg/L NaCI) I 2 3 Control 34 36 28 600 38 32 29 800 34 36 34 1000 30 30 32 1200 21 13 13 1400 8 5 5 Dunnett's MSD value: 2.711 PMSD: 8.4 Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses 4 31 33 30 30 19 8 MSD= PMSD= Test number: CLINaCICR

  1. 80 ----Test dates: Udobu 07-13,2008

,'),!, ------------\Jj Replicate number Survival Average reproduction Cueffidenl of Pcn*cnt 1 cUuctiun from (%) (offspring/female)

"ariutiuu l tuulrul CYv) 5 6 7 8 9 10 31 35 33 32 31 33 100 32.4 7.2 Not applicable


c----**-*** 34 30 34 32 32 32 100 32.6 7.5 -0.6 ------31 30 32 32 34 30 100 32.3 6.5 (J.J 32 32 32 32 29 29 100 30.8 4.3 -l.<J ---*-----l9 13 II 23 l8 21 100 17.1 2-1.7 -17.2 --------*-


3 7 3 8 10 10 100 6.7 3!L6

'----------------Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be dedarcd staustlcally significant in a whole effluent toxicity test Lower PMSD bound determined by USEPA (!01h percentile)

13%. Upper PMSD bound determined by USEPA (90th percentile)

47%. Lower and upper PMSD bounds were determined from the I Oth and 90th percentile. respectively, ofPMSD data from EPA's WET lnlcrlabu1 Jlory Variability Study (USEPA, 200 I a; US EPA, 200 I b). USEPA. 200 !a, 200! b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-0 1-004 and FPA-821 B 01-005. US Environmental Protection Agency, Cincinnati, OH. "ti (JQ \0 ... 0 ...... \0 0'1 Statistical Analyses .. J Environmental Testing Solutions, Inc. Ceriodaphnia Survival and ReEroduction Test-ReEroduction Start Date: 1017/2008 Test ID: CdNaCICR End Date: 10/13/2008 Lab ID: ETS-Envir. Testing Sol. Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Comments: Conc-mg!L I 2 3 4 5 6 D-Control 34.000 36.000 28.000 31.000 31.000 35.000 600 38.000 32.000 29.000 33.000 34.000 30.000 800 34.000 36.000 34.000 30.000 31.000 30.000 1000 30.000 30.000 32.000 30.000 32.000 32.000 1200 21.000 13.000 13.000 19.000 19.000 13.000 1400 8.000 5.000 5.000 8.000 3.000 7.000 Transform: Untransformed Conc-mg!L Mean N-Mean Mean D-Control 32.400 1.0000 32.400 600 32.600 1.0062 32.600 800 32.300 0.9969 32.300 1000 30.800 0.9506 30.800 *1200 17.100 0.5278 17.100 *1400 6.700 0.2068 6.700 Auxiliary Tests Ko1mogorov D Test indicates normal distribution (p > 0.01) Bartlett's Test indicates equal variances (p = 0.03) Min 28.000 29.000 30.000 29.000 11.000 3.000 Hypothesis Test (1-tail, 0.05) NOEC LOEC Dunnett's Test Treatments vs D-Control 1000 1200 Max CV% 36.000 7.157 38.000 7.542 36.000 6.535 32000 4.275 23.000 24.726 10.000 38.569 ChV TU J 095.44512 Sample ID: Sample Type: Test Species: 7 8 33.000 32.000 34.000 32.000 32.000 32.000 32.000 32.000 11.000 23.000 3.000 8.000 N t-Stat 10 10 -0.169 10 0.084 10 1.350 10 12.905 10 21.677 Statistic 0.7737003 12.0027618 MSDu MSDp 2. 71098343 0.08367233 Linear Interpolation (200 Resamples) Point SD 95%CL Skew !COS 990 77.815583 773.3125 1014.27169 -2.4281 1CIO 1022.62774 1!.2990174 1002.70019 1038.77377 -2.1436 !C15 1046.35036 9.57591142 1027.49595 1064.20095 0.0648 TC20 1070.07299 10.5377883 1048.11784 1091.92162 0.3416 JC25 1093.79562 11.9318091 1072.19759 1121.37833 0.5359 JC40 1164.9635 17.2015679 1138.07325 1205.85798 0.6225 IC50 1216.34615 20.7905444 1181.83123 1255.24757 0.1597 REF-RefToxicant NACL-Sodium chloride CD-Ceriodaphnia dubia 9 10 31.000 33.000 32.000 32.000 34.000 30.000 29.000 29.000 18.000 21.000 10.000 10.000 ------!-Tailed Isotonic Critical MSD Mean N-Mean 32.500 1.0000 2.287 2.711 32.500 1.0000 2.287 2.711 32.300 0.99311 2.287 2711 30.800 0.9477 2.287 2.711 17.100 0.5262 2.287 2.711 6.700 0.2062 Critical Skew K11rl 1.035 -0.03508 J 8 -0.1738142 15.0862722 MSB MSE F-Prob df 1192.29667 7.02777778 9.9E-32 5. 54 /0-07-08 Page .5 of 6 Species: Ceriodaphnia dubia

  1. 60 CONTROL 600 mg NaCl/L 800 mg NaCl/L 1000 mg NaCI/L 1200 mg NaCl!L 1400 mg NaCl!L STOCK Page 95 of96 Page 6 of6 Species:

Ceriodaphnia duhia 'f: &() CONTROL 600 mg NaCI/L 800 mg NaCI/L 1000 mg NaCI/L 1200 mg NaCIIL 1400 mg NaCI/L STOCK I Page 96 of 96 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ ___ 20DD_L-DAISY _ _IN 37381._ ______ _ Facility_ JYA -_§E:QUOYAH NUCLEAR PLANT _____ _ Locatio.!]_ .JjAMIL TO..ti_COUNT:L_ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 103 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD From YEAR I MO DAY I DAY 08 10 01 To 31 MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 LOW VOL. WASTE TREATMENT POND EFFLUENT NO DISCHARGE D ... NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION I NO. 0 0 0 DUIT OR THRU PLANT 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary . properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is . to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information. including the possibility of fine and imprisonment for knowing violations. TYPED OR P.RINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used EX TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 11 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO CODE Page 1 of 1 13 DAY PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Add res.§_ _E Q,_BOX 2000 ___________ _ -__ _§.ODD_X,_- DAISY _ _JN 37381._ ______ _ Locatio.n._ .J::!AMIL TO.!:!_ COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER 0 0 0 0 FFLUENT GROSS VALUE RON, TOTAL (AS FE) 0 0 CONDUIT OR THRU ENT PLANT 1 0 0 EFFLUENT GROSS VALUE TN0026450 YEAR From I 08 , . -I NAME!TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties tor submitting false information, including the possibility of fme and Imprisonment for knowing violations TYPED OR PRINTED ---------------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used 107 G DAY 31 METAL CLEANING WASTE POND EFFLUENT NO DISCHARGE I XX I *** NOTE: Read instructions before completinq this form. NO. IFREQ_UENCYI SAMPLE EX OF TYPE ANALYSIS ... TELEPHONE DATE I)""T ""'

  • Principal Environmental Engineer 423 I 843-6700 08 11 13 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA I --------------***

I NUMBER YEAR MO DAY Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Addres,L ___________ _ TN0026450 110 G -__ _§ODD..Y_- DAISY _ _IN 3738i_ ______ _ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER EFFLUENT Facility_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ locatio.!J..._ _!:!AMIL TO..!i_COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER

PERATURE, WATER DEG. NTIGRADE z 0 0 0 0 CONDUIT OR THRU ITI:>J:ATUENT PLANT 50050 1 0 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL 0 0 YEAR From 08 MINIMUM ********

NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate lhe information submitted. Based on my inquiry of the person or persons who manage lhe system, or those persons directly responsible for gathenng the information, the Information submitted is , lo lhe best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the poSSibility of fine and imprisonment for knowing violations_ TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used DAY 31 NO DISCHARGE I XX I *** NOTE: Read instructions before this form. QUALITY OR CONCENTRATION j NO. EX AVERAGE MAXIMUM ******** (.l...._d TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 11 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO CODE Page 1 of 1 13 DAY PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Addres_L ___________ _ -__ ....§.ODD...Y._- DAISY _ __JN 3738!._ ______ _ Facility_ JYA -.§!:QUOYAH NUCLEAR PLANT _____ _ Locatio.!l._ .J::!AMIL TOJ:i.COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ TN0026450 110 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR I MO I DAY I I YEAR I MO I DAY From 08 I 10 I 01 I To I 08 I 10 I 31 MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard NOTE: Read instructions before completinq this form. PARAMETER STATRE 7DAY CHR RIODAPHNIA 0 0 0 0 ENT GROSS VALUE NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED ---------- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used (} TELEPHONE DATE Principal Environmental Engineer 843-6700 08 11 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO Page 1 of 1 13 DAY PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 Addres_L ___________ _ -__ _§ODDY-DAISY _ _JN 3738L ______ _ Facility_ ...IYA -_§EQUOYAH NUCLEAR PLANT _____ _ Locatio.!}_ ..J::!AMIL TOl!.._COUNTY.._ _________ _ ATIN: Stephanie A. Howard PARAMETER DEBRIS, FLOATING (SEVERITY) 01345 0 0 EFFLUENT GROSS VALUE LAND GREASE VISUAL 0 0 EFFLUENT GROSS VALUE TN0026450 YEAR From I 08 I . -I -* I QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM ********

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance wtth a system designed to assure that qualtfied personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my tnquiry of the person or persons who manage the system. or those persons directly responsible for gathering the tnformation, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the posstbility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Prev1ous editions may be used 116 G 31 NO DISCHARGE [J *** DAY NOTE: Read instructions before completinq this form QUALITY OR CONCENTRATION I NO. IFREQ EX OF ANALYSIS AVERAGE MAXIMUM UNITS 0 ******** 0 9A G ClA--d TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 11 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO CODE Page 1 of 1 13 DAY PERMITTEE NAME/ADDRESS (Include Facititv Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMRJ MAJOR (SUBR 01) F-FINAL BACKWASH Form Approved. OMB No. 2040-0004 Addres_L_E.Q,_BOX_1900 ___ __§.ODDY-DAISY _ _JN 3738L ______ _ Facility_ JYA -_§E:QUOYAH NUCLEAR PLANT _____ _ Locatio_ll_ J:!AMIL TO!i_COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER IUt:Ct'(t,::,, FLOATING (SEVERITY) TN0026450 YEAR From L 08 . ---QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM ********

.. NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete_

I am aware that there are significant penalties for submitting false TYPED OR PRINTED I information, including the possibility of fine and impnsonment for knowing violations. COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used 117 G EFFLUENT NO DISCHARGE LJ *** DAY 31 NOTE: Read instructions before completinQ this form QUALITY OR CONCENTRATION I NO. I FREQUENCY EX OF SAMPLE TYPE AVERAGE MAXIMUM UNITS ANALYSIS VISUAL 1 I 31 ******** 0 0 9A a.-c-J TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 11 13 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRJ MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 TN0026450 ___ ..§.ODD..Y...- DAISY _ _IN 3738L ______ _ Facility_ .:JYA -..§!:QUOYAH NUCLEAR PLANT _____ _ Locatio.Q_ .J::jAMIL TO.!!. COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER N, DISSOLVED (DO) 00300 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 0 0 FLUENT GROSS VALUE OLIOS, SETTLEABLE 0 0 NDUIT OR THRU PLANT 0 0 YEAR From I 08 I . -I -* I MAXIMUM ******** NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance w1th a system des1gned to assure that qualif1ed personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the mtormat1on submitted is , to the best of my knowledge and belief, true, Site Vice President accurate, and complete I am aware that there are significant penalties for submitting false informatiOn, Including the poss1bllity of fine and imprisonment for knowing VIOlations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320*1 (REV 3/99) Previous editions may be used 118 G DAY 31 WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE I XX I *** NOTE: Read instructions before completinq this form. NITS QUALITY OR CONCENTRATION I NO. IFREQ EX OF ANALYSIS TELEPHONE Principal Environmental Engineer 423 843-6700 I 08 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE SAMPLE TYPE DATE 11 13 MO DAY Page 1 of 1 S58 081210 800-NPDES CORRESPONDENCE December 10, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR NOVEMBER 2008 Enclosed is the November 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sin?erely.: _ *.. I ) , . d 0 \tl_Qft,UO-A Stephanie A. Howard Principal Environmentaf Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, BR 4T-C A. A. Ray, WT 7C-K J. D. Smith, OPS 4A-SQN G. R. Signer, WT 6A-K EDMS, WT CA-K (Enclosure) DMR0811.doc Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 December 10, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, l & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR NOVEMBER 2008 Enclosed is the November 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. 0 d Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res.§_ ___________ _ -__ ....§.ODD...Y...- DAISY _ _IN 37381_ ______ _ Facility_ JYA -_§E.QUOYJlli_NUCLEAR PLANT _____ _ Locatio.!}_ .J:!AMIL TO.N._COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR DAY From 08 To 30 MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE D ... Form Approved. OMB No. 2040-0004 ATTN: Stephanie A Howard NOTE: Read instructions before completinQ this form. QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. I FREQ EX OF ANALYSIS PARAMETER UNITS MINIMUM AVERAGE MAXIMUM 04 , WATER DEG. ********

19.6 ******** 0 0 0 0 NAME/TITLE PRINCIPAL EXECUTIVE Certify under penalty of law that th1s document and all attachments were prepared under my , 0 }1 TELEPHONE DATE or superv1sion 1n accordance w1th a system des1gned to assure that qual1f1ed personnel I " . *fl.-LL _ (i:.A:_ Q, ! I Timothy P. Cleary !properly gather and evaluate the Information submitted Based on my 1nqU1ry of the person or 1 ' 1 persons who manage the system, or those persons responsible for gathenng the Principal Environmental Engineer I 423 I 843-6700 I 08 12 . . . information, the mformat1on submitted IS , to the best of my knowledge and belief, true, 1 S1te Vtce President

accurate, and complete.

1 am aware that there are sign1ficant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE i i 1 10 i information, mcluding the possibility of fine and imprisonment for know1ng v1olations. OFFICER OR AUTHORIZED AGENT I AREA I NUMBER YEAR I MO I DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3199) PreVious editions may be used Page 1 of 2 I I DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Date/Time Analyst Method 11/12/2008 @ 1212 0.32 mg/1 11/14/2008@ 1340 CLS TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 11/12/2008@ 1210 <0.10 mg/1 11/14/2008@ 1350 CLS TN EPA 8015 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Addres.L ___________ _ -__ __§ODD..Y.: DAISY _ _.IN 3738£_ ______ _ fscilitv_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ LocatiO!)_ .J::!AMIL TOli_COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER RINE, TOTAL RESIDUAL 0 0 E -C, RATE OF 0 0 GROSS VALUE TN0026450 YEAR From I 08 I *

  • I -* QUANTITY OR LOADING AVERAGE MAXIMUM UNITS ********

MINIMUM ******** 101 G DAY 30 DIFFUSER DISCHARGE EFFLUENT

      • NO DISCHARGE D ... NOTE: Read instructions before completinQ this form. QUALITY OR CONCENTRATION I NO. !FREQUENCY!

SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS 0.021 0.043 19 GRAB NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penally of law lhallh1s document and all attachments were prepared under my U .cUV\.L)Ct-t'--' TELEPHONE DATE dJrect1on or supervision 1n accordance with a system des1gned to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the Information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 08 12 10 information, the information subm1tted is . to the best of my knowledge and belief, true, Site Vice

accurate, and complete.

I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occurred:

1. H-150M (max. calc. cone. was 0.0411 mg/L--Iimit 0.050mg/L)
2. H-150M (low detection level analytical method was <0.020mg/L--Iimit 0.050mg/L)
3. MSW 101 (low detection level analytical method was 0.030mg/L--Iimit 0.20mg/L)

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved OMB No. 2040-0004 Addres,L ___________ _ TN0026450 -__ _§.ODD..Y..- DAISY _ _JN 3illL ______ _ Facility_ JYA NUCLEAR PLANT _____ _ locatio.!)_ ..J:!AMIL TOJi.COUNTY __________ _ YEAR ATTN: Stephanie A. Howard From I 08 I . . I --I PARAMETER QUANTITY OR LOADING AVERAGE ******** 7DAY CHR 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my direction or supervision 1n accordance with a system des1gned to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the informat1on submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the information, the information submitted is , to the best of my knowledge and belief, true. Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information. including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in November 2008. EPA Form 3320-1 (REV 3/99) Previous editions may be used 101 T DAY 30 BIOMONITORING FOR OUTFALL 101 EFFLUENT NO DISCHARGE D ... NOTE: Read instructions before completinq this form. QUALITY OR CONCENTRATION I NO. I FREQUENCY I SAMPLE EX OF TYPE TELEPHONE DATE Principal Environmental Engineer 843-6700 08 12 10 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT YEAR MO DAY Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res.§_ .J: 2000 ___________ _ -__ _§.ODDY-DAISY _ _IN 3?2§L ______ _ Facility_ JYA -SEQUOYAH NUCLEAR PLANT _____ _ Locatio.n_ .J:!AMIL TOl:LCOUNTY __________ _ ATIN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 103 G PERMIT NUMBER DISCHARGE NUMBER MUNI JKING PERIOD YEAR I MO I DAY I I YEAR I MO I DAY From 08 I 11 I 01 I To I 08 I 11 I 30 MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 LOW VOL. WASTE TREATMENT POND EFFLUENT

      • NO DISCHARGE D *** NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION

\ NO. SAMPLE 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 0 0 LAND GREASE 00556 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 1 0 0 LUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law thai this document and all attachments were prepared under my direction or supervision in accordance wilh a system designed to assure thai qualified personnel Timothy P. Cleary properly gather and evaluate the informalion submitted. Based on my inquiry of the person or persons who manage lhe system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President accurate. and complete. I am aware that there are significant for submitting false mformatlon, including the possibility of fine and Imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used TYPE AVERAGE ******** GRAB TELEPHONE DATE Principal Environmental Engineer 423 843-6700 08 12 10 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY CODE ---Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) F-FINAL Form Approved. OMS No. 2040-0004 Addres,L _E 20QQ.. ___________ _ TN0026450 -__ DAISY _ _IN 3738L ______ _ Facility_ JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio..!J_ _!:!AMIL TOJi.COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER PH 0 0 1sn1 ms, TOTAL SUSPENDED 0 0 0 0 EFFLUENT GROSS VALUE PHOSPHORUS, TOTAL (AS P) 00665 0 0 EFFLUENT GROSS VALUE TOTAL (AS CU) 0 0 FFLUENT GROSS VALUE RON, TOTAL (AS FE) 0 0 CONDUIT OR THRU ENT PLANT 0 0 EFFLUENT GROSS VALUE YEAR From I os I --I --I QUANTITY OR LOADING AVERAGE ******** NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used 107 G METAL CLEANING WASTE POND EFFLUENT NO DISCHARGE I XX I *** TELEPHONE DATE Principal Environmental Engineer 843-6700 08 12 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO -------Page 1 of 1 10 DAY PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres.L_E.SL_BOX_1900 ______ _ Facility_ JYA -.§E:QUOYAH NUCLEAR PLANT _____ _ Locatio.!!_ .J::!AMIL TOJi_COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRI TN0026450 110 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 PERMIT NUMBER DISCHARGE NUMBER I RECYCLED COOLING WATER MONITORING PERIOD EFFLUENT NO DISCHARGE I XX I *** From YEAR I YEAR I MO I DAY 08 To 08 11 30 NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION I NO. I FREQUENCY I SAMPLE 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 0 0 EFFLUENT GROSS VALUE LAND GREASE 0 0 DUITORTHRU ITCCATIUICIIJT PLANT 0 0 MINIMUM AVERAGE MAXIMUM ********

NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is . to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT ---*-----TYPE UNITS 04 TELEPHONE DATE 423 I 843-6700 08 12 10 AREA I NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L 2000 ___________ _ ______ _ Facilitv _ NUCLEAR PLANT _____ _ Locatio!!_ .J::!AMIL TOl:!.COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMRI TN0026450 YEAR From I 08 I --I --I MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION I NO. I FREQUENCY I SAMPLE EX OF TYPE STATRE 7DAY CHR ODAPHNIA 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Site Vice President TYPED OR PRINTED I Certify under penally of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief. true, accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT ---------------------------------- --***-------------------


COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used ANALYSIS TELEPHONE DATE 423 I 843-6700 08 12 10 AREA I NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Add res§_ ___________ _ ______ _ Facility_ .JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l.._ _!:!AMIL TOli.COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMR) TN0026450 116 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard YEAR I MO I DAY I I YEAR I MO I DAY 08 I 11 I 01 J To I 08 I 11 J 30 NO DISCHARGE D *** From NOTE: Read instructions before complelinQ this form. PARAMETER NO. IFREQUENCYI SAMPLE EX OF TYPE UNITS MINIMUM ANALYSIS BRIS, FLOATING (SEVERITY)

0 1 I 30 VISUAL 1 0 0 FFLUENT GROSS VALUE LAND GREASE VISUAL 0 0 NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE Timothy P. Cleary direction or supervision in accordance with a system designed to assure that qualified personnel . *\)....L_ . \ . properly gather and evaluate the informalion submitted. Based on my InqUiry of the person or persons who manage the system. or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 08 12 10 information, the information submitted is . to the best of my knowledge and belief. true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE --------**------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres,L __E.Q,_BOX 2000 ___________ _ ______ _ Facility_ JYA

  • SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!!_

.J::!AMIL TOJi.COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ TN0026450 117 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form OMB No. 2040-0004 ATTN: Stephanie A. Howard YEAR I MO I DAY I I YEAR I MO DAY From I 08 11 01 To 08 11 30 NO DISCHARGE D ... NOTE: Read instructions before completinq this form. PARAMETER NO. !FREQUENCY! SAMPLE EX OF TYPE ANALYSIS

IDEBRIS, FLOATING (SEVERITY) 0 1 I 30 VISUAL 0 0 ENT GROSS VALUE 0 0 ENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer 423 843-6700 08 12 10 information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possrbility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA l NUMBER YEAR MO DAY TYPED OR PRINTED CODE I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form (REV 3/99) Previous editions may be used Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L 2000 ___________ _ ___ ______ _ Facility_ JYA-_g:QUOYAH NUCLEAR PLANT _____ _ Locatio.!l_ .J::!AMIL TOli_COUNTy_ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT (OMRJ TN0026450 118 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR DAY From 08 To 30 MAJOR (SUBR 01) F-FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE Read instructions before completinq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION J NO. /FREQUENCY EX ' OF SAMPLE TYPE AVERAGE MAXIMUM MINIMUM (DO) 0 0 GROSS VALUE 0 0 EFFLUENT GROSS VALUE NAME!TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Site Vice President TYPED OR PRINTED ********

I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is . to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used ********

Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT ANALYSIS 19 TELEPHONE DATE 423 843-6700 08 12 10 I AREA 1 NUMBER YEAR MO DAY CODE ' I Page 1 of 1 S58 090217 800 -NPDES Correspondence February 17, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT (SON)-NPDES PERMIT NO. TN0026450-CORRECTION TO DISCHARGE MONITORING REPORT FOR DECEMBER 2008 During the review of the Biocide/Corrosion Treatment Plan (B/CTP) it was noted that three total residual chloride analyses had been omitted from the December 2008 Discharge Monitoring Report (DMR). Please see the attached corrected DMR page. It should be noted that the chlorine, total residual maximum value remained the same at 0.032mg/L and the chlorine, total residual average value increased from 0.017mg/L to 0.018 mg/L. Please contact me at (423) 843-6700 or by email at sahoward@tva.gov if you have any questions or need additional information. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah .Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 cc: D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, BR 4T-C U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 A. A. Ray, WT 7C-K B. A. Wetzel, OPS 4A-SQN G. R. Signer, WT 6A-K EDMS, WT CA-K (Enclosure) REVIEW/CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)-SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-CORRECTION TO DISCHARGE MONITORING REPORT FOR DECEMBER 2008 ORGANIZATION: Chemistry and Environmental DATE: 02/13/2009 oCA.. 11 Boo DOCUMENT PREPARED BY: Ann Hurt EDMS TRACKING NO.: S58 090213 802 NPDES Correspondence CONCURRENCES Name R c Signature -Comment Date v N Ann Hurt X zoo4 Stephanie A Howard X 2.. \ Alan K Barringer X v). Debra J Bodine X Beth A. Wetzel X ;2 Chris R Church X z Timothy P Cleary X I Stephanie A. Howard X z}ICl}ocy (for signature) INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended

purpose, logical in construction, and clear in meaning in the eyes of the recipient.

A concurrence signature indicates that the individual would be willing to sign the document for the agency. Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 February 17, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEOUOYAH NUCLEAR PLANT (SON)-NPDES PERMIT NO. TN0026450-CORRECTION TO DISCHARGE MONITORING REPORT FOR DECEMBER 2008 During the review of the Biocide/Corrosion Treatment Plan (B/CTP) it was noted that three total residual chloride analyses had been omitted from the December 2008 Discharge Monitoring Report (DMR). Please see the attached corrected DMR page. It should be noted that the chlorine, total residual maximum value remained the same at 0.032mg/L and the chlorine, total residual average value increased from 0.017mg/L to 0.018 mg/L. Please contact me at (423) 843-6700 or by email at sahoward@tva.gov if you have any questions or need additional information. Sincerely, MeutU_ Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L ....E.Q,_BOX 2000 ___________ _ ______ _ Facility_ .JYA -_g:QUOYAH NUCLEAR PLANT _____ _ Locatio.!l.._ .J:!AMIL TOl::!..COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT (OMRJ TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT Form Approved OMB No. 2040-0004 NO DISCHARGE U *** ATIN: Stephanie A Howard YEAR I MO I DAY I I YEAR I MO I DAY 08 I 12 I 01 I To I 08 I 12 I 31 From PARAMETER

HLORINE, TOTAL RESIDUAL 0 0 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the Information submitted.

Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the information. the information submitted is, to the best of my knowledge and belief. true, Site Vice President

accurate, and complete.

I am aware that there are srgnificant penalties for submitting false information, includmg the possibility of fine and imprisonment for knowing violations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occurred:

1. Biodetergent 73551 (max. calc. cone. was 0.017mg/L--Iimit 2.0mg/L)

EPA Form 3320-1 (REV 3/99) Previous editions may be used NOTE: Read instructions before completinQ this form. NO. IFREQUENCYI SAMPLE EX OF TYPE ANALYSIS 0 21 I 31 GRAB CALCTD CALC TO I TELEPHONE DATE Principal Environmental Engineer 423 843-6700 09 02 11 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY CODE Page 2 of 2 I Address Information Ship to: Mr. Patrick Cromer TDEC-Div. ofWater Pollution 6th Floor, L & C Annex 401 Church Street Nashville, TN 372431534 us 4238436700 Shipping Information Ship from: Ruth Ann Hurt TVA SEQUOY AH NUCLEAR PLANT SODDY DAISY, TN 37379 us 4238436714 Tracking number: 797348126247 Ship date: 02/19/2009 Estimated shipping charges: 40.35 Package Information Service type: First Overnight Package type: FedEx Envelope Number of packages: 1 Total weight: O.lLBS Declared value: O.OOUSD Special Services: Pickup/Drop-off: Give to scheduled courier at my location Billing Information Bill transportation to: Sender Your reference: P.O. no.: Invoice no.: Department no.: Please Note Fed Ex will not be responsible for any claim in excess of $100 per package, whether the result of loss, damage, delay, non*delivery, misdelivery, or misinformation, unless you declare a higher value, pay an additional charge, document your actual loss and file a timely claim. Limitations found in the current Fed Ex Service Guide apply. Your right to recoverfrom FedEx for any loss, including intrinsic value of the package, loss of sales, income interest, profrt, attorney's fees, costs, and other forms of damage whether direct, incidental, consequential, or speical is limited to the greater of $100 or the authorized declared value. Recovery cannot exceed actual documented loss. Maximum for items of extraordinary value is $500, e.g., jewelry, precious metals, negotiable instruments and other items lised in our Service Guide. Written claims must be filed within strict time limits; Consult the applicable FedEx Service Guide for details. The estimated shipping charge may be different than the actual charges for your shipment. Differences may occur based on actual weight, dimensions, and other factors. Consult the applicable or the F edEx Rate Sheets for details on how shipping charges are calculated. https://www.fedex.com/shippinglhtml/en/PrintiFrame.html 02/18/2009 Address Information Ship to: Mr. Mike Kelley Chattanooga EAC -Div. of Water Ship from: Ruth Ann Hurt TVA State Office Building, Suite SEQUOY AH NUCLEAR 550 PLANT 540 McCallie A venue Chattanooga, TN 374022013 us 423-843-6700 Shipping Information SODDY DAISY, TN 37379 us 4238436714 Tracking number: 796351781523 Ship date: 02119/2009 Estimated shipping charges: 4.52 Package Information Service type: Priority Overnight Package type: FedEx Envelope Number of packages: 1 Total weight: 0.1LBS Declared value: O.OOUSD Special Services: Pickup/Drop-off: Give to scheduled courier at my location Billing Information Bill transportation to: Sender Your reference: P.O. no.: Invoice no.: Department no.: Please Note FedEx will not be responsible for any claim in excess of $100 per package, whether the result of loss. damage, delay, non-delivery, misdelivery, or misinformation, unless you declare a higher value, pay an additional charge, document your actual loss and file a timely claim. Limitations found in the current F edEx Service Guide apply. Your right to recover from FedEx for any loss. including intrinsic value of the package, toss of sales, income interest, profit. attorney's fees, costs, and other forms of damage whether direct. incidental, consequential, or speical is limited to the greater of $100 or the authorized declared value. Recovery cannot exceed actual documented loss. Maximum for items of extraordinary value is $500, e.g., jewelry, precious metals, negotiable instruments and other rtems lised in our Service Guide. Written claims must be filed within strict time limits; Consult the applicable FedEx Service Guide for detailS. The estimated shipping charge may be different than the actual charges for your shipment. Differences may occur based on actual weight, dimensions, and other factors. Consult the applicable or the Fed Ex Rate Sheets for details on how shipping charges are calculated. https://www.fedex.com/shipping/html/en/PrintiFrame.html 02/18/2009 Address Information Ship to: To whom it may concern: Nuclear Regulatory Commission ATTN: Document Control Desk Washington, DC 20555 us 423-843-6700 Shipping Information Ship from: Ruth Ann Hurt TVA SEQUOY AH NUCLEAR PLANT SODDY DAISY, TN 37379 us 4238436714 Tracking number: 797348133411 Ship date: 02/19/2009 Estimated shipping charges: 4.52 Package Information Service type: Priority Overnight Package type: FedEx Envelope Number of packages: 1 Total weight: 0.1LBS Declared value: O.OOUSD Special Services: Pickup/Drop-off: Give to scheduled courier at my location Billing Information Bill transportation to: Sender Your reference: P.O. no.: Invoice no.: Department no.: Thank you for shipping online with Fedex ShipManager at fedex.com. Please Note Fed Ex will not be responsible for any claim in excess of $100 per package, whether the result of loss, damage, delay, non-delivery, misdelivery, or misinfotmation, unless you declare a higher value, pay an additional charge, document your actual loss and file a timely claim. Limitations found in the current F edEx Service Guide apply. Your right to recover from FedEx for any loss, including intrinsic value of the package, loss of sales, income interest. profrt, attorney's fees, casts, and other forms of damage whether direct, incidental, consequential, or speical-is limited to the greater of $100 or the authorized declared value. Recovery cannot exceed actual documented loss. Maximum for items of extraordinary value is $500, e.g., jewelry, precious metals, negotiable instruments and other items lised in our Service Guide. Written claims must be filed within strict time limits; Consult the applicable FedEx Service Guide for details. The estimated shipping charge may be different than the actual charges for your shipment. Differences may occur based on actual weight, dimensions, and other factors. Consult the applicable or the F edEx Rate Sheets for details on how shipping charges are calculated. https://www.fedex.com/shipping/html/en/PrintiFrame.html 02/18/2009 S58 090112 800-NPDES CORRESPONDENCE January 12, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR DECEMBER 2008 Enclosed is the December 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, I 0 d Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): cc: Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 D. J. Bodine, POB 2A-SQN C. R. Church, POB 2B-SQN T. P. Cleary, OPS 4A-SQN D. E. Pittman, BR 4T-C A. A. Ray, WT 7C-K J.D. Smith, OPS 4A-SQN G. R. Signer, WT 6A-K EDMS, WT CA-K (Enclosure) DMR0812.doc Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384*2000 January 12, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR DECEMBER 2008 Enclosed is the December 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555

  • PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different)

___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Add res§_ ___________ _ ___ ______ _ Facilitv _ _IVA -..§!:QUOYAH NUCLEAR PLANT _____ _ Locatio.!}_ _.!jAMIL TO..ti.COUNTY __________ _ ATTN Stephanie A. Howard PARAMETER . DIFF. BETWEEN SAMP. & UPSTRM DEG.C 1 w 0 EFFLUENT GROSS VALUE PH 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 0 0 0 0 GROSS VALUE , IN CONDUIT OR THRU ENT PLANT 1 0 0 EFFLUENT GROSS VALUE TN0026450 YEAR From I 08 I * -I -* I QUANTITY OR LOADING AVERAGE MAXIMUM UNITS ********

101 G DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE LJ *** DAY 31 NOTE: Read instructions before completinq this form. QUALITY OR CONCENTRATION i NO. [FREQUENCYj-SAMPLE I EX I OF I TYPE I ANALYSIS MINIMUM AVERAGE MAXIMUM UNITS 31 I 31 I MODELD ******** ******** 13.3 0 04 I NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my ClcL!vvuU-kd TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel 09 I I Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Engineer 423 843-6700 01 12 Information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete I am aware that there are s1gnif1cant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE I j I I 1nformat1on, 1ncluding the possibility of fine and imprisonment for knowing vtolations OFFICER OR AUTHORIZED AGENT AREA ! NUMBER \YEAR I MO I DAY TYPED OR PRINTED I I CODE ! i ' COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attacHments here) No closed mode operation.

The following information is included in an attachment:

1. CCW data 2. veliger monitoring data EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 2 '

DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/1 0/2008 @ 1220 0.17mg/1 12/12/2008@ 0956 KLM TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/10/2008@ 1215 <0.10 mg/1 12/12/2008 @ 1006 KLM TN EPA 8015 Mean# of Water Temp. Mean# of Water Temp. LOCATION SUB NOTES:% Sample Date ZM/m3 % Settlers ("C) Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY Clams/m3 ("C) Clam 11/21/2008 0 0 12 11/16/2008 230 12 In plant RCW Dick Adcock 11/24/2008 31 0 11 11/24/2008 138 11 In plant RCW Dick Adcock 12/4/2008 0 0 10 12/04/2008 15 10 lnplant RCW Dick Adcock 12/8/2008 0 0 9 12/08/2008 0 9 In plant RCW Dick Adcock 12/15/2008 0 0 9 12/15/2008 0 9 lnplant RCW Dick Adcock 12/22/2008 0 0 9 12/22/2008 0 9 In plant RCW Dick Adcock 12/29/2008 0 0 9 12/29/2008 0 9 In plant RCW Dick Adcock 01/09/2008 0 0 8 01/09/2009 0 8 In plant RCW Dick Adcock PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Addres_L _E 9.c._BOX..1_900 ___________ _ -__ _§ODD..Y_- DAISY _ _Jti]7381.._ ______ _ Facility_ .JYA -_§l:QUOYAH NUCLEAR PLANT _____ _ Locatio!l_ .J:!AMIL TO.!i_COUNTL_ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ TN0026450 101 G YEAR From LOS ----MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE CJ *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUALITY OR CONCENTRATION I NO. I FREQUENCY I SAMPLE EX OF TYPE ANALYSIS MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 0 0.017 LORINE, TOTAL RESIDUAL

GRAB 18 I 31 0.032 19 URE-C, RATE OF 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this docu. ment and all attachments w.ere prepared under my r ... * ..

  • 1'\ rd .* . d or supervision in accordance w*th a system des1gned to assure that qualified personnel

}fr h.G(j' , " UA (IJ_(/).,<...)Cu Timothy p. Cleary properly gather and evaluate the 1nformat1on submitted. Based on my Inquiry of the person or u.c'>j) '-"'-"-'\' personswho manage the system, or those persons responsible for gathenng the Principal Environmental Engineer 8 3 6700 . . . information, the 1nformat1on subm11ted IS , to the best of my knowledge and bel1ef. true, 4

  • S1te V1ce President

!accurate, and complete 1 am aware that there are significant pena111es for submitt1ng false SIGNATURE OF PRINCIPAL EXECUTIVE _________ ___jlnformalion, 1nclud1ng the poss1bd1ty of f1ne and 1mpnsonment for know1ng VIOlations. OFFICER OR AUTHORIZED AGENT NUMBER TYPED OR PRINTED I TELEPHONE DAY DATE 09 01 12 1YEAR MO ---------------- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occurred:

1. Biodetergent 73551 (max. calc. cone. was 0.017mg/L--Iimit 2.0mg/L)

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 2 of 2 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L ___________ _ _________ _ _ _ _ DAISY _ _JN 3738L ______ _ Facility_ .:JYA

  • SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l_

.J::!AMIL TOl:!_COUNT:L_ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMR) TN0026450 101 Q MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE II Form Approved. OMB No. 2040-0004 ATTN: Stephanie A Howard From , __ , . _ 1 _

  • NOTE Read instructions before completinq this form. PARAMETER QUALITY OR CONCENTRATION MAXIMUM MINIMUM MAXIMUM UNITS BORON, TOTAL ********

<0.20 19 1022 1 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law thallhis document and all attachments were prepared under my cu.. d TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons responsible for gathenng the Principal Environmental Engineer 423 843-6700 09 01 information, the information submitted IS to the best of my knowledge and belief, true, Site Vice President I accurate. and complete I am aware that there are sigmficant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE 1nformat1on. 1nclud1ng the possibility of f1ne and 1mpnsonment for know1ng VIOlations. OFFICER OR AUTHORIZED AGENT I AREA TYPED OR PRINTED __j_ ___ ___ ____l__C_Q_QE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Boron was sampled on 10/01/2008@ 1120. EPA Form 3320-1 (REV 3/99) Previous editions may be used YEAR MO Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Add res.§_ _E.Q;_BOX_1.900 ___________ _ -__ _§_ODD_1_- DAISY _ _]N 37381._ ______ _ Facility _ _s:/A -_§!:QUOYAH NUCLEAR PLANT _____ _ [ TN0026450 I [ PERMIT NUMBER _j Locatio.!}_ _t!AMIL TO.!:!._COUNTL_ _________ _ ATTN: Stephanie A. Howard From 1 --1 *-1 -* PARAMETER STATRE 7DAY CHR RIODAPHNIA 1 0 0 1 0 0 EFFLUENT GROSS VALUE NAMEITITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary I Certify under penalty of law that th1s document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathenng the 1nformat1on. the information submitted is . to the best of my knowledge and belief, true. Site Vice President

accurate, and complete I am aware that there are significant penalties for submittmg false f------------------------jlnformatiOn, including the possib1l1ty of fine and Imprisonment for knowing violations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in December 2008. EPA Form 3320-1 (REV 3/99) Previous editions may be used 101 T BIOMONITORING FOR OUTFALL 101 EFFLUENT 31 NO DISCHARGE CJ *** DAY NOTE: Read instructions before completinq this form.

Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NO. IFREQ EX OF ANALYSIS TELEPHONE T DATE 423 AREA T CODE I 843-6700 09 01 12 NUMBER IYEARI MO I DAYJ Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res.§_ ___________ _ -__ _§_ODDY-DAISY _ _IN 37384_ ______ _ Facility_ ..JYA -_g:QUOYAH NUCLEAR PLANT _____ _ Locatio.Q_ _jjAMIL TOJ!.COUNT!::_ _________ _ ATIN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) r--* --TN002645c)-I PERMIT NUMBER I 103 G YEAR Fromj 08 I *-, -* MAJOR (SUBR 01) F-FINAL Form Approved. OMB No 2040-0004 LOW VOL. WASTE TREATMENT POND EFFLUENT NO DISCHARGE CJ *** NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. I FREQUENCY I SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM ********

7.2 ******** 8.3 0 0 1;,suuu;,s. TOTAL SUSPENDED I 0 0 DUITORTHRU PLANT 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1 Cert1fy under penalty of law that th1s document and all attachments were prepared under my I 1

  • d or superv1s1on 1n accordance w1th a system des1gned to assure that qualified personnel

(/{ , \C<..--L Timothy P Cleary "'""' """" ""'""""' '"'"m'"oo '""m'"" '"" oo m, '"'"'" o<"" m F persons who manage the system or those persons responsible for gathenng the Prtncipal Environmental Engineer 1nformat1on, the 1nformat1on subm1tted IS , to the best of my knowledge and belief, true, Site Vice President accurate and complete I am aware that there are Slgn1f1cant penalt1es for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE 1nformat1on, 1nclud1ng the poss1b1hty of f1ne and 1mpnsonment for know1ng VIOlatiOns OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED --COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used UNITS ANALYSIS 12 0 14 I 31 GRAB TELEPHONE DATE 423 843-6700 09 01 12 I AREA l NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L __f ___________ _ -__ _§.ODD_.Y_- DAISY _ _JN 3738L ______ _ Facilitv_JYA-_§!:QUOYAH NUCLEAR PLANT _____ _ Locatio.[!_ _!:!AMIL TO.!:!_ COUNTY __________ _ ATIN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMR! TN0026450 107 G YEAR From I 08 I -I -. I MAJOR (SUBR 01) F-FINAL METAL CLEANING WASTE POND EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinQ this form. QUANTITY OR LOADING PARAMETER QUALITY OR CONCENTRATION I NO. I FREQ EX OF PH 0 0 0 1042 1 0 0 FFLUENT GROSS VALUE RON, TOTAL (AS FE) 0 , IN CONDUIT OR THRU ENT PLANT 0 0 ENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Site Vice President AVERAGE ANALYSIS

I Certify under penalty of law that thiS document and all attachments were prepared under my I ( t (\ .. d-d TELEPHONE r DATE ' direction or supervision 1n accordance w*th a system designed to assure that qualified personnel Cn ,\1lCt...rtA.....e..- l l* ... A. . -1 properly gather and evaluate the mformat1on submitted. Based on my inquiry of the person or u:.>f-1

  • persons who manage the system, or those persons dlfectly respons1ble for gathering the Principal Environmental Engineer O OQ O 12 1nformat1on.

the mformat1on subm1tted 1s , to the best of my knowledge and belief, true, 423 843-670 1 accurate, and complete. I am aware that there are significant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED

  • CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location 1f Different)

___ _ Add res.§_ ___________ _ -__ _20DD..Y_- DAISY _ _IN 37381._ ______ _ Facility_ ...JYA -..§E:QUOYAH NUCLEAR PLANT _____ _ Locatioll_ .J:!AMIL TOJi.COUNTY __________ _ ATTN: Stephanie A Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (OMR) TN0026450 YEAR From I os I . -I -. I I 110 G i I DISCHARGE NUMBER I DAY 31 MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. /FREQUENCY/ SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM E, WATER DEG. 04 ********

0 0 0 0 1 0 0 FFLUENT GROSS VALUE RINE, TOTAL RESIDUAL 0 0 PRINCIPAL EXECUTIVE OFFICER I' Cert1fy under penalty of law that this document and all attachments were prepared under my -------* drrectron or supervrsron 1n accordance wrth a system des1gned to assure that qualified personnel Timothy P. Cleary 'properly gather and evaluate the 1nformat1on submitted Based on my 1nqU1ry of the person or persons who manage the system, or those persons drrectly responsrble for gathering the tnformation, the information submitted is , to the best of my knowledge and bel1ef, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalt1es for submitttng false information, including the possibility of fine and imprisonment for knowing violationsc TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used AVERAGE MAXIMUM ANALYSIS

04 cu.d TELEPHONE DATE Principal Environmental Engineer 12 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT DAY ------Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Add res§_ ___E Q,_BOX_1900 ___________ _ -__ __20DDY-DAISY _ _IN 37381._ ______ _ Facility_ .JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!!_ _!:!AMIL TO.!i_COUNTY._ _________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) DISCHARGE MONITORING REPORT (OMR) I --.,-{o-rl DISCHARGE NUMBER j TN0026450 PERMIT NUMBER MONITORING PERIOD From I I I To MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE [xxJ *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. I FREQUENCY I SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ********

NAME!TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my 0 .rilewcu d directton or superviston 1n accordance wtth a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED --------'- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used ANALYSIS 23 TELEPHONE DATE 423 843-6700 09 01 12 I AREA l NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L _.E.\1_BOX_1900 ___________ _ -__ DAISY _ _..::rN 37381_ ______ _ Facility_ JYA -_§EQUOYAH NUCLEAR PLANT _____ _ Locatio.[!_ _!:jAMIL TOl:!_COUNTY __________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATI.ON SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (OMRJ r--moo26450-l I 116 G I I PERMIT NUMBER II DISCHARGE NUMBER I MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard NO DISCHARGE D ... YEAR DAY From I 08 I --I -* I 31 NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. I FREQUENCY I SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS FLOATING (SEVERITY) 1 I 31 VISUAL ********

0 0 9A 0 0 ENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my 0.. TELEPHONE DATE d1rection or supervision in accordance with a system designed to assure that qualif1ed personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer 423 843-6700 09 01 12 information, the information submitted is , to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I Information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 Add res§_ ___________ _ ______ _ Facility_ .JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!.!_ .J:!AMIL COUNTY __________ _ ATTN: Stephanie A. Howard PARAMETER

uc.cro;1;
:.,

FLOATING (SEVERITY) 0 0 FFLUENT GROSS VALUE IL AND GREASE VISUAL 0 0 TN0026450 YEAR From I 08 ; --I --AVERAGE MAXIMUM UNITS ********

117 G 31 NO DISCHARGE D *** DAY NOTE: Read instructions before completinQ this form. QUALITY OR CONCENTRATION I NO.I FREQUENCY I SAMPLE EX OF TYPE UNITS ANALYSIS I i AVERAGE MAXIMUM MINIMUM ******** 0 0 1 I 31 VISUAL ******** 9A NAMErriTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared Ulider my 0 .clkwo.--. cL TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 09 01 12 information, the information submitted is , to the best of my knowledge and belief. true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA l NUMBER YEAR MO DAY TYPED OR PRINTED CODE ---------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres.2_ ___________ _ ______ _ Facility_ JYA -_§!:QUOYAH NUCLEAR PLANT _____ _ Locatio!)_ _!:!AMIL TO.!i.COUNTY __________ _ ATTN: Stephanie A Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRJ TN0026450 118 G YEAR DAY From I 08 I --I --I 31 MAJOR (SUBR 01) F-FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completino this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. !FREQUENCY! SAMPLE TYPE MAXIMUM UNITS AVERAGE ' -j MAXIMUM ___ ---t-------i-----------

UNITS MINIMUM DISSOLVED (DO) , IN CONDUIT OR THRU ENT PLANT 1 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is . to the best of my knowledge and belief, true, Site Vice President

accurate, and complete.

I am aware that there are significant penalties for submitting false Information, mcluding the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED --------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Q TELEPHONE DATE Principal Environmental Engineer 423 843-6700 09 01 12 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY CODE Page 1 of 1}}