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| number = ML13289A198
| number = ML13289A198
| issue date = 08/11/2010
| issue date = 08/11/2010
| title = Sqn Annual Water Withdrawal Updates for July 2010
| title = SQN Annual Water Withdrawal Updates for July 2010
| author name = Church C R
| author name = Church C
| author affiliation = Tennessee Valley Authority
| author affiliation = Tennessee Valley Authority
| addressee name = Cromer P
| addressee name = Cromer P
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=Text=
=Text=
{{#Wiki_filter:S58 100811 800-NPDES CORRESPONDENCE August 11, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement  
{{#Wiki_filter:S58 100811 800- NPDES CORRESPONDENCE August 11, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6"' Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
& Compliance Section 6"' Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534  


==Dear Mr. Patrick Cromer:==
==Dear Mr. Patrick Cromer:==
SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JULY 2010 Enclosed is the July 201 o Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.
 
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete.
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JULY 2010 Enclosed is the July 201 o Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: B. E. Brickhouse, LP 5U-C C. R. Church, OPS 4A-SQN S. A. Howard, OPS 5N-SQN K. Langdon, POB 2B-SQN D. B. Nida, LP 5U-C A A. Ray, WT 11A-K G. R. Signer, WT 6A-K B. A Wetzel, OPS 4A-SQN G. J. Wynn, POB 2B-SQN K. M. Hodges (EDMS), LP 2V-C Tennessee Valley Authority.
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc:
P::*st (Jj1ce Sex 2CDO. Scddt Oaisf', Tenressee 3138.:!-2-JCC August 11,2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement  
B. E. Brickhouse, LP 5U-C                                           A A. Ray, WT 11A-K C. R. Church, OPS 4A-SQN                                             G. R. Signer, WT 6A-K S. A. Howard, OPS 5N-SQN                                             B. A Wetzel, OPS 4A-SQN K. Langdon, POB 2B-SQN                                               G. J. Wynn, POB 2B-SQN D. B. Nida, LP 5U-C                                                  K. M. Hodges (EDMS), LP 2V-C
& Compliance Section 6'* Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534  
 
Tennessee Valley Authority. P::*st (Jj1ce Sex 2CDO. Scddt Oaisf', Tenressee 3138.:!-2-JCC August 11,2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6'* Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534


==Dear Mr. Patrick Cromer:==
==Dear Mr. Patrick Cromer:==
SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JULY 2010 Enclosed is the July 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.
 
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete.
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JULY 2010 Enclosed is the July 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sincerely, 4_t;Uz Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Sincerely, 4_t;Uz Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission A TIN: Document Control Desk Washington, D.C. 20555 REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT July DMR ORGANIZATION:
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission A TIN: Document Control Desk Washington, D.C. 20555
Environmental DOCUMENT PREPARED BY: Bethany Hyatte DATE: 8/5/2010 CONCURRENCES Name R c Signature  
 
-Comment Date v N ' S. A. Howard X _4twheutM._  
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT                       July DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Bethany Hyatte DATE: 8/5/2010 CONCURRENCES Name             R     c                 Signature - Comment                 Date v     N S. A. Howard             X             _4twheutM._
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                                      /~:?/~
Originator will determine the review/concurrence assignment.
C. R Church                   X tO ~urf l.JL()
REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization.
                                      '-                     c.;-
CONCURRENCE:
INSTRUCTIONS:     Originator will determine the review/concurrence assignment.
Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended  
REVIEW:       Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization.
: purpose, logical in construction, and clear in meaning in the eyes of the recipient.
CONCURRENCE:       Indication of agreement with the document as a whole.
A concurrence signature indicates that the individual would be willing to sign the document for the agency.
Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
PERMmEE NAME/ADDRESS (Include Facilitv Name/Location if Oifferenl)
 
Jllame __ Til_ S_EOUOYAH
PERMmEE NAME/ADDRESS                   (Include Facilitv Name/Location if Oifferenl)                           NATIONAl POllUTANT DISCHARGE EliMINATION SYSTEM (NPDES)                                                                              Form Approved.
__ _
MAJOR DISCHARGE MONITORING REPORT                          (OMR)
___ P_,Q.__Il_OX2_QOO_
Jllame_ _ Til_A~ S_EOUOYAH_I'JIJCL_E~R ~~N!_ _ _ _                                                                                                                                                    (SUBR 01)                                      OMB No. 2040-0004
__ _ _ _ _ ______ _ _____ ttNJE...RQEOCU6:28_*SQNl
_A_ddfe~~  ___ P_,Q.__Il_OX2_QOO_ __ _ _ _         _______ _
_______ _ ____ SQD!LY _cDI\[S_'L lli ______ _ £9Qjjt;y_
c~~~~~~B~R                                        DISCHARG~~~M~ER 1
_
_____ttNJE...RQEOCU6:28_*SQNl _ _ _ _ _ _ _ _                                                                                                                                                        F- FINAL
_______ _ J.Q@!Q_
_ _ _ _ SQD!LY _cDI\[S_'L lli >>~-              ______ _                                                                                                     11                                      DIFFUSER DISCHARGE
____________
£9Qjjt;y_ _ .TYA_:_S.EQUQ'ffiH_l.ll,K:L.f.l\KP.!._A_~T _______ _
_ NATIONAl POllUTANT DISCHARGE EliMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMR) 11 1 MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT Form Approved.
t~TING            ro[D~~t                          ~
OMB No. 2040-0004 ATTNo stepnanie A. Howard I YEAR MO MO QA From 10 I 07 01 ro[ 10 07 I 31 *** NO DISCHARGE  
J.Q@!Q_ _H_8.M!!:um_..cOY~TY____________ _                                                                                                                                                           EFFLUENT I YEAR      MO                                          MO        QA
[l *** NOTE: Read instructions before this. form. ><
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ATTNo stepnanie A. Howard                                                                                   From            10  I   07            01                 10         07     I 31             *** NO DISCHARGE NOTE: Read instructions before campletin~  this. form.
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                                                                                            . * .* I                                    *-*          rc.......~......'* . ,.*:.-.J;15' is; i1?,~ .* 20..
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I.*.         * *****'**         MDAVO *.HeDAILYMX MG/L             MONTHL      VI  GRAB FLOW,IN CONDUIT OR THRU                                  SAMPLE                    ********                      1635                  03                                            ********                   ********             **       0   I 31 I 31       RCORDR TREATMENT PLANT                                      MEASUREMENT 50050        1        0    0                            PERMIT                  ********                  ru:rul                    MGD                                                                                                            I CONTIN EFFLUENT        GROS~_VALUE                          REQUIREMENT                                          DAILY MX .                                                                                                                                    uous
I ********
                                                                                                                                                                                                                          ~
. **.* I ********
            ~E-PRINCIPAL          EXECUTIVE OFFICER ICertilyunderpen<~ltyofl<~wthOII*t.hisdocum. entandallattadlmentswereprepa.,... undt!rmy                                                         ~~-
1635 50050 1 0 0 PERMIT ********
r
ru:rul EFFLUENT REQUIREMENT DAILY MX . *-* 03 MGD 3 19 I , , **** ,,., **. ,
                                                                                                                                                                                            /                                           TELEPHONE                     DATE
3a1 'l 1 oo . , ,*',
                  ****-*------------- ---*-*--* ------~~- direction or superv1s1on in accord<ince wtth a system des~gned to assure that qualified personnel                           /    ~                                                                          -
MG/L <6 <6 19 rc ....... ...... ' **. ,.*:.-.J;15' is;  
01~ com~lete. ~are                                                    sub~it ing                                                                                                      -~-..-t-Christopher R. Church                     properly gather and evaluate the Information submitted. Ba5ed on my 1nqu1ry of the person or                                     ,.,.:;
.* 20 .. I.*. * *****'**
person5 who manage the system, or those persons directly responsibl&ior gathenng the                             Sequoyah Site           ice     President
MDAVO *. HeDAILYMX MG/L ********
                              .   *         .               inlormallon, the Information Sl.lbmltled IS, to the best of my knowledge and belief, true,                                                                                       843*7001         10       08     09 uoyah S1te. VIce Prestdent               accurate,                 I am       that there are significant penalties for             false           SIGNATURE OF PRINCIPAL EXECUTIVE                                   ______
********  
                  ----- -
** 0 1/ 51 GRAB -* MONTHLY GRAB -----0 i 1131 GRAB MONTHL VI GRAB 0 I 31 I 31 RCORDR I CONTIN uous EXECUTIVE OFFICER entandallattadlmentswereprepa.  
* information, 1nclud1ng the poss1b111ty affine and 1mpnsonmentfor knowing v1olat1ons.                             OFFICER OR AUTHORIZED AGENT                                       NUMBER       YEAR       M       UAY T!_~~~-9R PR~-~~ED                                                                                                                                                                                                            ___       _ __
, ... undt!rmy  
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (1/eference all attachments her No closed mode operation. The following information is included in attachments: CCW data, veliger monitoring data, and thermal discussion.
/ TELEPHONE DATE ****-*-------------
  - - - - - - -*           ~cc=c---~-=--c---o--
---*-*--*
Page 1 of 2 EPA Form 3320-1 (REV 3199)                   Previous editions may be used
direction or superv1s1on in accord<ince wtth a system to assure that qualified personnel  
 
/ -Christopher R. Church properly gather and evaluate the Information submitted.
DMR Attachment CCWData CCWTRENCH Extractable Petroleum Date/Time Collected       Hydrocarbons             Analysis Date/Time     Analyst Method No water would come out of the pump. No sample could be obtained.
Ba5ed on my 1nqu1ry of the person or ,.,.:; person5 who manage the system, or those persons directly responsibl&ior gathenng the Sequoyah Site ice President  
CCWCHANNEL Extractable Petroleum Date/Time Collected       Hydrocarbons             Analysis Date/Time     Analyst Method 07/14/2010@ 1042             <0.10 mg/1             07/15/2010@ 2125         JOB   EPH
. * . inlormallon, the Information Sl.lbmltled IS, to the best of my knowledge and belief, true, 843*7001 10 08 09 uoyah S1te .. VIce Prestdent  
 
: accurate, I am that there are significant penalties for false SIGNATURE OF PRINCIPAL EXECUTIVE
Mean# of                               NOTES:%
______  
Mean# of             Water                           Water                SUB Sample Date         %Settlers Temp. ("C) Sample Date Asiatic           LOCATION  LOCATION Gravid Asiatic    COLLECTED BY ZM/m3                                            Temp. ("C)
------* information, 1nclud1ng the poss1b111ty affine and 1mpnsonmentfor knowing v1olat1ons.
Clamslm3                                   Clam 11/03/2009   133       0         16     11/03/2009     76       16       lnplant   RCW                         CMW 11/10/2009   417     6.1       16     11/10/2009     25       16       lnplant   RCW                         CMW 11/17/2009   269       0         16     11/17/2009     0       16       lnplant   RCW                         CMW 11/24/2009     36     50         15     11/24/2009     18       15       lnplant   RCW                         CMW 12/01/2009     32       0       13.5     12/01/2009     0     13.5     lnplant   RCW                           WE 12/08/2009     38       0         11     12/08/2009     0       11       lnplant   RCW                         CMW 01/05/2010     0       0         6     01/05/2010     0       6       lnplant   RCW                             B 01/12/2010     0       0         5     01/12/2010     0       5       lnplant   RCW 01/19/2010     0       0         6     01/19/2010     0       6       lnplant   RCW                             p 01/26/2010     32       0         7.5     01/26/2009     0       7.5     lnplant   RCW                           NRT 02/02/2010     0       0         7     02/02/2010     0       7       lnplant   RCW                       MSW/WDT 02/09/2010     0       0         8     02/09/2010     0       8       lnplant   RCW                         BUTC 02/16/2010     0       0         5     02/16/2010     0       5       In plant   RCW                           BJ 02/23/2010   11.7     0         7     02/23/2010     0       7       In plant   RCW                           BJ 03/02/2010     0       0         6     03/02/2010     0       6       In plant   RCW                           PB 03/09/2010     0       0         8     03/09/2010     0       8       In plant   RCW                         MJW 03/16/2010     0       0         10     03/16/2010     0       10       In plant   RCW                           BC 03/23/2010     14       0         11     03/23/2010     0       11       In plant   RCW                           BC 03/30/2010     0       0         14     03/30/2010     0       14       In plant   RCW                         BAPO Apr-2010                                                                                                   no samples collected May-2010                                                                                                   no samples collected Jun-2010                                                                                                   no samples collected Jul-2010                                                                                                   no samples collected
OFFICER OR AUTHORIZED AGENT NUMBER YEAR M
 
___ _ __ UAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (1/eference all attachments her No closed mode operation.
For ten days in July, the daily maximum 24-hour average temperature river temperature was above 30.5 C. In all instances, Sequoyah complied with State of Tennessee NPDES Permit No. TN0026450 (effective date September 1, 200S), Part 1, A. Effluent Limitations and Monitoring Requirements for Outfa\1101, page 2 of 25 bullet 5 which states ,"The daily maximum 24-hour average river temperature is limited to 30.5 C. Since the state's criteria makes exception for ~xceeding the value as a result of natural conditions, where the 24-hour average ambient temperature exceeds 29.4 C and the plant is operated in helper mode (full operation of one cooling tower, at least three lift pumps, per operating unit) the maximum temperature may exceed 30.S C. In no case shall the plant discharge cause the 1-hour average river temperature at the downstream edge of the mixing zone to exceed 33.9 C without the consent of the permitting authority."
The following information is included in attachments:
The below table is provided as a demonstration of when Sequoyah met these requirements of the permit during the month of July.
CCW data, veliger monitoring data, and thermal discussion.  
24-hour           24-hour         24-hour       Maximum 1       Maximum 1         Number of average           average         average       Hr average       Hr average         Cooling downstream         ambient         ambient       Downstream       Downstream         Tower Lift temperature       temperature     temperature     temperature       temperature       Pumps in July         {Celsius)     {Fahrenheit)       {Celsius)     {Fahrenheit)       {Celsius)         Service
-------*
                                            >29.4 deg C                       < 33.9 deg C 9           30.8             87.5           31.1             88.1             31.2               6 10           30.6             87.1           30.6             87.4             30.8               6 16           30.7             87.2           30.4             87.8             31.0               6 24           31.2             88.1           31.4             88.7             31.5               6 25           31.2             88.2           31.3             88.0             31.1               6 26           30.7             87.3           31.0             88.1             31.2               6 27           30.7             87.2           30.8             87.1             30.6               6 29           30.8             87.4           31.1             88.0             31.1               6 30           31.1             87.9           31.8             89.5             31.9               6 31           31.3             88.3           32.1             88.7             31.5               6
EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 2 DMR Attachment CCWData CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained.
 
CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 07/14/2010@
PERMITTEE NAME/ADDRESS             (Include Facility Name/Location if Different}                             NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
1042 <0.10 mg/1 07/15/2010@
MAJOR                                      Form Approveel.
2125 JOB EPH Mean# of Water Mean# of Water SUB NOTES:% Sample Date ZM/m3 %Settlers Temp. ("C) Sample Date Asiatic Temp. ("C) LOCATION LOCATION Gravid Asiatic COLLECTED BY Clamslm3 Clam 11/03/2009 133 0 16 11/03/2009 76 16 lnplant RCW CMW 11/10/2009 417 6.1 16 11/10/2009 25 16 lnplant RCW CMW 11/17/2009 269 0 16 11/17/2009 0 16 lnplant RCW CMW 11/24/2009 36 50 15 11/24/2009 18 15 lnplant RCW CMW 12/01/2009 32 0 13.5 12/01/2009 0 13.5 lnplant RCW WE 12/08/2009 38 0 11 12/08/2009 0 11 lnplant RCW CMW 01/05/2010 0 0 6 01/05/2010 0 6 lnplant RCW B 01/12/2010 0 0 5 01/12/2010 0 5 lnplant RCW 01/19/2010 0 0 6 01/19/2010 0 6 lnplant RCW p 01/26/2010 32 0 7.5 01/26/2009 0 7.5 lnplant RCW NRT 02/02/2010 0 0 7 02/02/2010 0 7 lnplant RCW MSW/WDT 02/09/2010 0 0 8 02/09/2010 0 8 lnplant RCW BUTC 02/16/2010 0 0 5 02/16/2010 0 5 In plant RCW BJ 02/23/2010 11.7 0 7 02/23/2010 0 7 In plant RCW BJ 03/02/2010 0 0 6 03/02/2010 0 6 In plant RCW PB 03/09/2010 0 0 8 03/09/2010 0 8 In plant RCW MJW 03/16/2010 0 0 10 03/16/2010 0 10 In plant RCW BC 03/23/2010 14 0 11 03/23/2010 0 11 In plant RCW BC 03/30/2010 0 0 14 03/30/2010 0 14 In plant RCW BAPO Apr-2010 no samples collected May-2010 no samples collected Jun-2010 no samples collected Jul-2010 no samples collected For ten days in July, the daily maximum 24-hour average temperature river temperature was above 30.5 C. In all instances, Sequoyah complied with State of Tennessee NPDES Permit No. TN0026450 (effective date September 1, 200S), Part 1, A. Effluent Limitations and Monitoring Requirements for Outfa\1101, page 2 of 25 bullet 5 which states ,"The daily maximum 24-hour average river temperature is limited to 30.5 C. Since the state's criteria makes exception for the value as a result of natural conditions, where the 24-hour average ambient temperature exceeds 29.4 C and the plant is operated in helper mode (full operation of one cooling tower, at least three lift pumps, per operating unit) the maximum temperature may exceed 30.S C. In no case shall the plant discharge cause the 1-hour average river temperature at the downstream edge of the mixing zone to exceed 33.9 C without the consent of the permitting authority."
DISCHARGE MONITORING REPORT                    (DMRJ
The below table is provided as a demonstration of when Sequoyah met these requirements of the permit during the month of July. 24-hour 24-hour 24-hour Maximum 1 Maximum 1 Number of average average average Hr average Hr average Cooling downstream ambient ambient Downstream Downstream Tower Lift temperature temperature temperature temperature temperature Pumps in July {Celsius)  
!'!~me__TY_A-_ 5_EOUOYJI:H_rl~L:_E~~I.JIN!_                  __ .                                                                                                                                (SUBR01)                                  OMB No_ 2040-0004 A_d!1[~__p_.Q._[I:QX_2QO(L - - - - - * - -- .... - - - - - - - -
{Fahrenheit)  
I P;R~~~~~:B~R31 D!SCHARG:~~!RJ
{Celsius)  
____ U_NJEQ&#xa3;F_K:Uf!:28.-:.S.ONL _______ _                                                                                                                                                         F- FINAL
{Fahrenheit)  
_ ___ _ _ S.QpQY ~A!S_Y... TN~?~- _ _ _ _ _ -*-* _                                                                                                                                               DIFFUSER DISCHARGE
{Celsius)
.F.iiQ!iQ. _TYl\_:_SJ;QYQY8H_NYC!.EAR. eLAl'H ______ _
Service >29.4 deg C < 33.9 deg C 9 30.8 87.5 31.1 88.1 31.2 6 10 30.6 87.1 30.6 87.4 30.8 6 16 30.7 87.2 30.4 87.8 31.0 6 24 31.2 88.1 31.4 88.7 31.5 6 25 31.2 88.2 31.3 88.0 31.1 6 26 30.7 87.3 31.0 88.1 31.2 6 27 30.7 87.2 30.8 87.1 30.6 6 29 30.8 87.4 31.1 88.0 31.1 6 30 31.1 87.9 31.8 89.5 31.9 6 31 31.3 88.3 32.1 88.7 31.5 6 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different}
J.QqtiQ_ ____H_A_MJ1T_QN_J;;Q!JNIY____________ _                                                                                                                                                  EFFLUENT
TY_A-_
                                                                                                                                                                                                      *** NO DISCHARGE          D ...
__ .  
ATTN: Stephanie A. Howard 50060             0     0 EFFLUENT GROSS VALUE TEMPERATURE- C, RATE OF CHANGE 82234       1     0 EFFLUENT GROSS VALUE 0                            PERMIT REQUIREMENT
-----*---.... --------____ U_NJEQ&#xa3;F_K:Uf!:28.-:.S.ONL
                                                                                    ********                                                                   ******.**, !.:. .,*. ........ : **;E[ *******
_______ _ _ ___ _ _ S.QpQY  
                                                                                                                                                                                                -~-:;t :~;i:'.
...
j f-     I CONTIN rALCTD uous.                       ,
_____ -*-* _ .F.iiQ!iQ.
r---                                                   SAMPLE                                                                                                                                                                                  1 MEASUREMENT f---+.
_TYl\_:_SJ;QYQY8H_NYC!.EAR.
PERMIT REQUIREMENT                                                                                       I:\.'                          -"""
eLAl'H ______ _ J.QqtiQ_
SAMPLE MEASUREMENT
____ H_A_MJ1T_QN_J;;Q!JNIY
                                                                                                                                                                                                                                          ~---+-
____________
I
_ ATTN: Stephanie A. Howard 50060 0 0 EFFLUENT GROSS VALUE ----------*-*-
                                                                                                                                                        ~r<
TEMPERATURE-C, RATE OF CHANGE 82234 1 0 0 EFFLUENT GROSS VALUE r---PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT I SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT
PERMIT REQUIREME~T SAMPLE MEASUREMENT PERMIT REQUIREMENT                                                                                        lI,;-.
********
                                                                                                                                                          .J SAMPLE MEASUREMENT 1-:---t---*
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ I MAJOR (SUBR01)
I ..
F-FINAL DIFFUSER DISCHARGE EFFLUENT
~-                                                                                                                                                                                            ::i*;~t*~'
*** NO DISCHARGE
PERMIT
******.**  
                                    --          1 REQUIREMENT SAMPLE             1                           1                           !                 1                     1>         < c                           1               1     1 MEASUREMENT                                                                                                                                                                                           --t--- --
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PERMIT REQUIREMENT I .. 1-:---t---* --1 SAMPLE 1 1 ! 1 1> < c 1 1 1 MEASUREMENT  
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EXECUTIVE QFFICE:"i~ d1rect1on PRINCIPAL ----------------
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Christopher R. Church 1-Cert1fy .under pen.ally of1nlaw or superviSIOn         that.this d~ment accotdance           an*d*. all with a system       ~ttachments designed               P.repar.
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we." that to assure           ed under qualified properly gather and evaluate the infonnalion submitted. Based on my inqu1ry of the person or my personnel        z;              r.;z;.~*                      TELEPHONE *--    --,-----DATE 1--,....::__ :_-=-. --~
__ __L __ _:___J \1\iAMEfriTLE PRINCIPAL EXECUTIVE 1-Cert1fy
                                                                                                                                                                                                                                                                            --~
.under pen.ally of law that.this an*d*. all we. " P. repar. ed under my z;
Sequoyah S1te Vtce Prestdent persons who manage the system, or !hose persons directly responsible for gathering the linformalion, \he information submitted 1s. to the best of my knowledge and belief, true, aa::ISate, and complete. 1am aware that there are s1gnific.:ant penalties for subrnltttng talse information, 1nclud1ng the possibility of flne and impr1sonment for knowing Vtolallons.
---:;---TELEPHONE
Sequoyah Site Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGE NT 423 AREA t  1 843-7001          10
--,-----DATE
                                                                                                                                                                                                                                    --N._U_M_B_E_R-----1-YEAR --
-----*-----------------
08 MO--
d1rect1on or superviSIOn 1n accotdance with a system designed to assure that qualified personnel
09 DAY IL._ _ _ _.:.TY.:.:.PED OR_Pil."":r*o. *-               I                                                                                                                                                               ~~;;;        -                                 .--
*--1--,....:: __ :_-=-. ---* Christopher R. Church properly gather and evaluate the infonnalion submitted.
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attacnments ner The following injections occurred: 1. Floguard MS6236 (max. calc. cone. was 0.062mg/L-Iimit 0.2mg/L) 2. Biodetergent 73551 (max. calc. cone. was 0.017mg/L--Iimit 2.0mg/L) 3. Spectrus CT1300 (max. calc. cone. was 0.039mg/L-Iimit 0.050mg/L) 4. Spectrus CT1300 (low detection level analytical method was <0.050mg/L-Iimit 0.050mgll)
Based on my inqu1ry of the person or persons who manage the system, or !hose persons directly responsible for gathering the Sequoyah Site Vice President
EPA Fonn 3320-1 (REv     .)l"lml         Previous editiOit>> may J.X: u>>c-u                                                                                                                                                                   Page 2 of 2
. . . linformalion,  
 
\he information submitted 1s. to the best of my knowledge and belief, true, 423 1843-7001 1 0 08 09 Sequoyah S1te Vtce Prestdent aa::ISate, and complete.
PERMITIEE NAME/ADDRESS             (fr?Ciude Facilitv Name/location if Different)                         NATIONAL POLLUTANT DISCHARGE ELIMINATION SVSTEM (NPDES)                                                                                                    Form Approved.
1 am aware that there are s1gnific.:ant penalties for subrnltttng talse SIGNATURE OF PRINCIPAL EXECUTIVE t I information, 1nclud1ng the possibility of flne and impr1sonment for knowing Vtolallons.
MAJOR DISCHARGE MONITORING REPORT                                        (DMRJ Rame__ TlfA_: $1;~9_Y~H_Nl)CLEAR ~U\N!, ___ _                                                                                                                                                                   (SUBR 01)                                            OMB No. 2040-0004 MQr~ ___P...Q ...&sect;PX200Q           __ ~---- - * - -----
OFFICER OR AUTHORIZED AGE NT AREA --N._U_M_B_E_R-----1-YEAR  
CJ!~~~~!R                                    II rnscHARO~ ~~M:ER I
--MO--DAY L. ____ .:.TY.:.:.PED OR_Pil."":r*o.  
-   _  ~    _I!N_TEEQEFJCU~&.S.Q_Nl_ _ _ _ _ _ _ _ _
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F- FINAL
-. COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attacnments ner The following injections occurred:
______ SQ.PQY .:..P.8.!SY. INM~.-- _ _ _ - --- _                                                                                                                                                                  BIOMONITORING FOR OUTFALL 101 E~QLmL.      __TYl\_:_SECYQ"LBtfll!J.CLEARJ~J.A.NJ _.. -* _ _ _
: 1. Floguard MS6236 (max. calc. cone. was 0.062mg/L-Iimit 0.2mg/L)  
EFFLUENT t~TING~t
: 2. Biodetergent 73551 (max. calc. cone. was 0.017mg/L--Iimit 2.0mg/L)  
.!:fK~ti.Q_  _H_A_M!!,T.QNSQ!.!fiD'_____ .. _ _ _ _ _ _ _
(max. calc. cone. was 0.039mg/L-Iimit 0.050mg/L)  
To \i 07 AITN: stephanie A. Howard                                                                               From I YEAR 10    I MO 07        01 MO I
: 4. Spectrus CT1300 (low detection level analytical method was <0.050mg/L-Iimit 0.050mgll)
DA)'
EPA Fonn 3320-1 (REv .)l"lml Previous editiOit>>
31 I   ~* NO DISCHARGE                  D ...
may J.X: u>>c-u 3. Spectrus CT1300 Page 2 of 2 PERMITIEE NAME/ADDRESS (fr?Ciude Facilitv Name/location if Different)
NOTE: Read instructions before completinq this form.
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_____ .. ______ _ AITN: stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SVSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ II I YEAR MO MO DA)' I I From 10 I 07 01 To \i 07 I 31 MAJOR (SUBR 01) F-FINAL Form Approved.
J r.OMPOS EFFLUENT_GROSS              II_AL~-            I   RE::::ENT           .I                       .I                       I                 I       MIMINI.JI                     I                             I                       I                                 --
OMB No. 2040-0004 BIOMONITORING FOR OUTFALL 101 EFFLUENT NO DISCHARGE D ... NOTE: Read instructions before completinq this form. PARAMETER  
MEASUREMENT PERMIT                                                                                                       -~*-**
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ANALYSIS E 7DAY CHR ----t"-SAMPLE --****. HNIA MEASUREMENT 0 0 PERMIT GROSS VALUE REQUIREMENT IC25 STATR-E 7DAY CHR --SAMPLE ***** .. . ' -PIMEPHALES MEASUREMENT I I I I '" *--TRP6C 0 0 1"\II;Oy  
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.I .I I I MIMINI.JI I I I --MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT I 1-----
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OFFIC~R -. Idirection
1-PERMIT REQUIREMENT I SAMPLE MEASUREMENT
                                            -----         Cartify.undar pen~l~y o~ lawtha1 thisd.ocument and a.II aUachment:s.were pre. " ' " " **
--PERMIT REQUIREMENT
                                                                                                                                                  ' under my Of" superviSIOn 1n acoordance w1th a system des1gned to assure that qualified personnel
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TELEPHONE DATE___ **~
-----+ --*---*-**--
                                                                                                                                                                                                                                                                              ~-*-J;;J***
----*-*--
Christopher R. Church                   properly gather and evaluate the Information subm*tted. Based on my 1nqu1ry of the person or persons who manage u-.e system, or those persoos d*raclly respoos1ble for gathenng the                                     Sequoyah Site             ice   p   tdent
-*--NAME/TITLE PRINCIPAL EXECUTIVE
                          .   .         .               mformat*on, the information submitted IS , to the best of my knowledge and bel~ef. true,
. I Cartify.undar lawtha1 thisd.ocument and a. II aUachment:s.were pre. "'""' ** under my 4-TELEPHONE t DATE ___
* 423         843-7001               10      08     09
------direction Of" superviSIOn 1n acoordance w1th a system des1gned to assure that qualified personnel *--
                                                        ~~ccurate, and complete. I am aware that there~ Significant penalties fDf slilmitting false                                                                                                               *--***-~---
* -*----Christopher R. Church properly gather and evaluate the Information subm*tted.
Sequoyah S1te Vtce_ President          .. _                                                                                                                      SIGNATURE OF PRINCIPAL EXECUTIVE                                       I I
Based on my 1nqu1ry of the person or persons who manage u-.e system, or those persoos d*raclly respoos1ble for gathenng the Sequoyah Site ice p tdent . . . mformat*on, the information submitted IS , to the best of my knowledge and true,
I
* 423 843-7001 1 0 08 09 Sequoyah S1te Vtce_ President
  ~                                                      tniOfmatlon, 1nc1ud.ng the poss1btllly affine and tmpnsonmentlor knowing v*otat*ons.                                     OFFICER OR AUTHORIZED AGENT                               AREA           NUMBER             YEAR     MO     DAY
... _
.                   TYPED OR PRINTED                                                                                                                                                                                                       CO.IlE....l...________ .,. __ .               _   *--
and complete.
LC:COO:M-cM=ENc:T=s AND EXPLANATION OF ANY VIOLATIONS               rReFerence all attachments het Toxicity was not sampled in July 2010.
I am aware that Significant penalties fDf slilmitting false SIGNATURE OF PRINCIPAL EXECUTIVE I
EPA Form 3320-1 (REV 3199)                 Previous editions may be used                                                                                                                                                                                             Page 1 of 1
_--_ __ _ ___ _ __________
 
-*--tniOfmatlon, 1nc1ud.ng the poss1btllly affine and tmpnsonmentlor knowing v*otat*ons.
PERMITTEE NAMEJADDRESS (Include Facility Name/Location if Different)                                        NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ                                                                                                          Form Approved MAJOR DISCHARGE MONITORING REPORT                                      (DMR)
OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY . TYPED OR PRINTED CO.IlE....l...________  
N.a!ll_e__TIJA- S_E~~'f!!.H_l'll)CI,.EAR Ptj\N!_ __ _                                                                                                                                                                 (SUBR 01)                                              OMB No. 204o-0004
.,. __ . _ *--LC:COO:M-cM=ENc:T=s AND EXPLANATION OF ANY VIOLATIONS rReFerence all attachments het Toxicity was not sampled in July 2010. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITTEE NAMEJADDRESS (Include Facility Name/Location if Different)
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F- FINAL ANNUAL MONITORING (PCBS)
_ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved OMB No. 204o-0004
                                                                                                                  ~                p~qRING P;mt                                                    ~
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                                                                          ~                                                                                                                                                                                              1---+--- -                                       I
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                                                                                                                                                                                              ~                      'f.t#                          ;---2ElEPHONE-                      -- - -
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~                      _____                ----iinform..ahon, 1nclud1ng the possibility of fine arxl imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT                                                                        YEAR        MO_- DAY L_____,_             TYPED OR PRINTED         _L *- - - - - - - - - - - -
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments net PCBs were sampled on 6/30/2010.
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments net PCBs were sampled on 6/30/2010.
EPA Form 3320*1 (REV 3199} Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT I-'------'---
EPA Form 3320*1      (REV 3199}        Previous editions may be used                                                                                                                                                                                                          Page 1 of 1
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----H 423 843-7001 10 08 09 I YEAR MO_-DAY Page 1 of 1 PERMIITEE NAME/ADDRESS (Include FaciJitv Name/Location if Different)
PERMIITEE NAME/ADDRESS                    (Include FaciJitv Name/Location if Different)                              NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)                                                        MAJOR                                                  Form Approved.
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_______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) F-FINAL Form Approved.
ATTN: Stephanie A_ Howard                                                                                            From                10      I MP 07i(                        ro[    10 MP 07        I pey 31
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==Dear Mr. Patrick Cromer:==
==Dear Mr. Patrick Cromer:==
SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR AUGUST 2010 Enclosed is the August 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. On August 21 and 23, 2010, verbal notifications were made for the Towerbrom 960 release that occurred on August 20, 2010. This was followed by a written submission on August 24, 2010. A copy of the written submission is attached.
 
If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments weffl prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR AUGUST 2010 Enclosed is the August 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. On August 21 and 23, 2010, verbal notifications were made for the Towerbrom 960 release that occurred on August 20, 2010. This was followed by a written submission on August 24, 2010. A copy of the written submission is attached.
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete.
If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
I certify under penalty of law that this document and all attachments weffl prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sincerely Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Sincerely
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C 20555 cc: B. E. Brickhouse, LP 5U-C C. R. Church, OPS 4A-SQN S. A Howard, OPS 5N-SON K. Langdon, POB 2B-SON D. B. Nida, LP SU-C A. A. Ray, WT 11A-K G. R. Signer, WT 6A-K B. A Wetzel, OPS 4A-SQN G. J. Wynn, POB 2B-SQN K. M. Hodges (EDMS), LP 2V-C IV Tennessee Valley Authority, Post Ot'1ce Box 2*:00. Sodd'J SlaiS'J.
~!&
Tannessee 3732.!-2000 September 9, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement  
Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
& Compliance Section 61" Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534  
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C 20555 cc:
B. E. Brickhouse, LP 5U-C                                   A. A. Ray, WT 11A-K C. R. Church, OPS 4A-SQN                                     G. R. Signer, WT 6A-K S. A Howard, OPS 5N-SON                                      B. A Wetzel, OPS 4A-SQN K. Langdon, POB 2B-SON                                      G. J. Wynn, POB 2B-SQN D. B. Nida, LP SU-C                                          K. M. Hodges (EDMS), LP 2V-C
 
Tennessee Valley Authority, Post Ot'1ce Box 2*:00. Sodd'J SlaiS'J. Tannessee 3732.!-2000 September 9, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1
6 " Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534


==Dear Mr. Patrick Cromer:==
==Dear Mr. Patrick Cromer:==
SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR AUGUST 2010 Enclosed is the August 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. On August 21 and 23,2010, verbal notifications were made for the Towerbrom 960 release that occurred on August 20, 2010. This was followed by a written submission on August 24, 2010. A copy of the written submission is attached.
 
If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR AUGUST 2010 Enclosed is the August 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. On August 21 and 23,2010, verbal notifications were made for the Towerbrom 960 release that occurred on August 20, 2010. This was followed by a written submission on August 24, 2010. A copy of the written submission is attached.
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete.
If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.  
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
/
Sin~y,                      /
Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
C---~lf{q Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk
* Washington, D.C. 20555 PERMITIEE NAME/ADDRESS (Include Facility Name/LocatiOn if Different) ffilm_e_
* Washington, D.C. 20555
_TVA_:_
 
JIH !"I!CL!!AR
PERMITIEE NAME/ADDRESS                    (Include Facility Name/LocatiOn if Different)                              NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)                                                                              Form Approved MAJOR DISCHARGE MONITORING REPORT (DMR) ffilm_e_ _TVA_:_ ~~ovJIH!"I!CL!!AR 1'1*~-~ __ _                                                                                                                                                              (SUBR 01)                                      OMB No. 2040-0004 Mctre_g;_p_,_g_JlQX2.Q91L          ~ ~---          ________ _
__ _ Mctre_g;_p_,_g_JlQX2.Q91L ________
5 I p;~~~~~:s~R                      J IDISCHARG:~~M~ER I
_ _(IN_TfBQEFj!;;UiiU!l&sect;QNL
_(IN_TfBQEFj!;;UiiU!l&sect;QNL _ _ _ _ .. _ _                                                                                                                                                      F- FINAL
____ .. __ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved OMB No. 2040-0004
_ _ _ _ SQQID' .o.Pi\!SL IN ~>!i4_ . _ _ _ _ .* _                                                                                                                                                            DIFFUSER DISCHARGE fi<:Jtily_ _T)/jl. ~--SEOYQYf!H_NJJC!.fAR.etAJ'iT _ _ _ _ _
____ SQQID' .o.Pi\!SL IN
.bQ~;;;:~_t[Q_ _H_ti_Mlbl__Q_N...CQ!Jfil.Y____________ _
. ____ .* _ fi<:Jtily_
ATTN: stephanie A. Howard                                                                                        From 10 I I YEAR      MO r:qRING 08      01      To m!l I    10 MO 08 I 31 I PAX EFFLUENT
_T)/jl.
                                                                                                                                                                                                                *** NO DISCHARGE            D ...
____ _
r--**
_H_ti_Mlbl__Q_N...CQ!Jfil.Y
TEMPERATURE, WATER PEG.
____________
CENTIGRADE I
_ I J I I I YEAR I MO r:qRING m!l MO I PAX I DIFFUSER DISCHARGE EFFLUENT From 10 08 01 To I 10 08 31 *** NO DISCHARGE D ... ATTN: stephanie A. Howard r--** TEMPERATURE, WATER PEG. CENTIGRADE I 00010 Z 0 0 PERMIT ""'****"**
00010          Z    0    0                                  PERMIT                  ""'****"**                    *****f*** -,            ~~~    1  * -~&#xa3;&#xa3;*-*'-'-'"''--      -*-;*.: ..**_,_.,~      ,,, __ ,        ---        '  ----      '              SEE  I CKREQ IINSTREAMMONITORING                                        REQUIREMENT                                .          .*                      I            I                                                                                                        PERMIT TEMPERATURE, WATER DEG.                                      SAMPLE                    ****"'***                    "'*****u                                                                                            38.3                    I    0      31 I 31  RCORDR CENTIGRADE                                                MEASUREMENT                                                                                                                                                                      04 00010            1    0    0                                  PERMIT                  ********          I            **~~=***"
*****f***
-, 1 *
-*-;*.: ..**
,,, __ , ---' ----' IINSTREAMMONITORING REQUIREMENT . .
* I I SEE I CKREQ TEMPERATURE, WATER DEG. SAMPLE ****"'***
"'*****u 38.3 CENTIGRADE MEASUREMENT 00010 1 0 0 EFFLUENT GROSS VALUE TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 s 0 EFFLUENT GROSS VALUE ------------**-
PH 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 00556 1 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 0 GROSS VALUE PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
* SAMPlE MEASUREMENT PERMIT REQUIREMENT
: SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPlE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PE:RMIT REQUIREMENT
********
I I ********
I ******"'* .. ****11:***
; I -**"'*****
I ... ,.. ..... * ** .. **** 1'---::;*,
I*_
__ 1nformat1011, InclUding 1he poss1b1h\y of f1ne and 1mpnsonment for knowing VIOlations.
__ 1nformat1011, InclUding 1he poss1b1h\y of f1ne and 1mpnsonment for knowing VIOlations.
OFFICER OR AUTHORIZED AGENT AREA 1 NUMBER YEAR MO DAY __ TYPEOORPRINTEO
felse                SIGNATURE OF PRINCIPAl EXECUTIVE OFFICER OR AUTHORIZED AGENT                     AREA I
_ moE..L..._.
1 NUMBER       YEAR     MO   DAY J
__ -*-. COMMENTS AND EJCPLANATlON OF ANY VIOLATIONS  
_ _TYPEOORPRINTEO                                      _                                                                                                                                                                        moE..L..._.           __     -*-         .
/Reference all attachments het Operations performs visual inspections for floating debris and oil and grease during all backwashes.
COMMENTS AND EJCPLANATlON OF ANY VIOLATIONS /Reference all attachments het Operations performs visual inspections for floating debris and oil and grease during all backwashes.
Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilftv Namell..acattan if Different}
EPAFonn3320~~~iV3~199~1-------p~,-.-wou~--s~ed~<~km~s-m.~y~bec-u-s~ed~--------------------------------------------------------------------------~-
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMRI MAJOR (SUBR 01) Form Approved.
Page 1 of 1
OMS No. 2040-0004
 
>Lame __ TI/A__:_
PERMITTEE NAME/ADDRESS              (Include Facilftv Namell..acattan if Different}                            NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ                                                                            Form Approved.
S_EOU()_YJIH_I\IU51,1'l\R
MAJOR
___ _
>Lame__TI/A__:_ S_EOU()_YJIH_I\IU51,1'l\R I'L~'T_ ___ _                                                                        DISCHARGE MONITORING REPORT                            (DMRI                                                      OMS No. 2040-0004 (SUBR 01)
B..Q.lt2.QOO_
A_cJ~_re_g)_p_,Q. B..Q.lt2.QOO_ _ _      __ _ _ _ _ _ _ _ _
_ _ _ ________
_ _ _ _t[NT~~QEFJl;Uilcl85QNL _ _ -* -
_ ____
* - - - *
__ -* -__ _ *---** SQPQY.:..P.81SY" Tf'i.fl:!l!<!
* SQPQY.:..P.81SY" Tf'i.fl:!l!<!_______ _
_______ _ [
[        P:~o~!::R                        II  DJscHARG;              ~~M~ER I ~~=~sH
II DJscHARG; I
.E~Kjjjty_ _T\LA.:_SWY9'ffilil"JY.C!.E.A.R.1?1A..I'_H_____ _
_T\LA.:_SWY9'ffilil"JY.C!.E.A.R.1?1A..I'_H
p:rmt                        -~
_____ _
t t 19~~tl9__1-!.Af'4!!.1Q.N_1;QY'IT(_____________ _
__ 1-!.Af'4!!.1Q.N_1;QY'IT(
---*-- -.- - -- ---[><
_____________
EFFlUENT ATTN:   Stephanie A. Howard                                                                                  From YEAR 10 I 08MoB~    ONITjRING 01          TO 10 I !E-'!B.          MQ 08 I  pey 31          .-  NO DISCHARGE D      ...
_ t tONITjRING p:rmt -EFFlUENT YEAR I I !E-'!B. MQ I pey D ATTN: Stephanie A. Howard From 10 08 01 TO 10 08 31 .-NO DISCHARGE
NOTE Read Instructions before completln~ ttlls rorm.
... NOTE Read Instructions before ttlls rorm. [> ---*---. --------< ---0-;;:U:-:AN=T=rr::-Y;-:O::R:-:l::O::A-::D-::IN::Gc-----,-------,O::U-cA:-:Lc:ITY:::-:O:::Rc:Cc:0 7N:::CE:::N:::T::R::A:::TIO'=N--
                                                                                        ---0-;;:U:-:AN=T=rr::-Y;-:O::R:-:l::O::A-::D-::IN::Gc-----,-------,O::U-cA:-:Lc:ITY:::-:O:::Rc:Cc:07N:::CE:::N:::T::R::A:::TIO'=N-- - - -
---* No: \FREOUENcvTsAMPLE-
* No:    \FREOUENcvTsAMPLE-
\ EX OF I TYPE AVERAGE I MAXIMUM I UNITS MINIMUM I AVERAGE I MAXIMUM I UNITS l ANALYSIS
                                                                                                                                                                                                                                              \ EX        OF      I    TYPE AVERAGE            I      MAXIMUM            I  UNITS                MINIMUM                I      AVERAGE          I    MAXIMUM      I  UNITS      l        ANALYSIS        .
. **"'**"'**
SA*~lE                      **"'"'*"'"'"'
! *****"'*"'
                                                                                                                *****"'*"'          !      *"'              **"'*"**"'            l      "'*******        l         o       I     9A       ! o-r 31T-V1Si.JAL MEASUREMI:.NI 01345       1     0     0                       /-- PERMIT                         ********                  ********                 '"'               ***"'****.*. _* :. : - .:;&#xa3;'- *'"******.          .,REPORT          PAss*o t----r--;::=--*         11 VISUAL  ----1 REQUIREMEt*-
! *"' **"'*"**"'
                                                                                                                                                                                                      *? ...                                              ~""
l "'*******
y*<;,                <              ,,      MOTOTA~-+--'*-    FAIL*1
l o I 9A ! o-r-11-31T-V1Si.JAL AVERAGE MAXIMUM 01345 1 0 0 84066 1 0 0 EFFLUENT GROSS VALUE SA* MEASUREMI:.NI
                                                                                                                                                                                                                                                            .. *****~
/--PERMIT REQUIREMEt*-
PERMIT SAMPLE                      ***11*1111*
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPlE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPlE MEASUREMENT PERMIT REOUIREMENJ
                                                                                      ***11**11*         I          n 0                                      *****11**                     *"'****11*            *****"'11*   ! ** I o ' 1 f 31 I ...                 __
**"'"'*"'"'"'
VISUAL MEASUREMENT 84066      1     0      0                              PERMIT REQUIREMENT
**"'**"'*"'
                                                                                      ..........          I REPORT                  YES=1 NO=O
'"' PAss*o t----r--;::=--*
                                                                                                                                                                                                                                      . ~.
1 ----1 FAIL*1 ********  
SEE        VISUAL EFFLUENT GROSS VALUE                                                                                        MOTOTAL                                                                                                                                    PERMIT I SAMPlE MEASUREMENT PERMIT REQUIREMENT                                      . I                                             I SAMPLE MEASUREMENT PERMIT 1---+--
********  
REQUIREMENT SAMPlE MEASUREMENT f-----+----1---*-
***"'****.*  
                                                                                                                                                      !;tT'f"~i:;l.;~c,~;;:;~,;(r/-*11 PERMIT REOUIREMENJ              I                     ****I*._--**-
.. _* :..::-. :;&#xa3;'-*'"******.
* __ **-1
*? ... .,REPORT 1 VISUAL PERMIT ***11*1111*
                                                                                                                * . . * *. : . . &#xa3; _ : _: <: ::**;~*~;~,:*:tf;:{~\rr .:~l:;?i-;*,:*.*,?,~ 1 rili:':::_.*;;~?)F .:HJ*.:* ::*
I 0 y*<;, < ,,
I            I      I        --+- I SAMFL.~::
..  
                                                                  --                                                                                                                                                                                ~-                  ~
***11**11*
MEASUREMENT PERMrr f--
n *****11**
REQUIREMENT SAMPLE l____
*"'****11*
MEASUREMENT o_.: ._.~.~- -- --'-----'
*****"'11*
MIT 1----f---l----
! ** I o ' ... __ 1 f 31 I VISUAL ..........
r - .... ~ ... ::~A:EIH l - ..                        1 jNAME!TITLE PRINCIPAL EXECUTIVE OFFICER I Certi_fy under.pena!ty ottawthal .lhrs~ment and a. II* a_llachm~nts wef.a- praparadundermy lELEPHONE       --f=-
I . I REPORT MOTOTAL YES=1 NO=O I *"*"'*****
                                                                                                                                                                                                                                                          --r--   DATE    *---~
.. SEE VISUAL PERMIT I *----1---+--f-----+----1---*-
                                                                                                                                                                                                                                                                      ----r---
I ****I*._--**-
~
** __ **-1 I I * * -* ... * *. :..&#xa3;_ ::_:: <:  
  ~        - - - *------ * - - - - - - -                    dire-cl.lon or superviSion 1n accoroaf!CU w1th a systl!lm designed to aSSl!re that qualified personnel                                                                   ----- ----*
.:HJ*.:*  
Chnstopher R Church                           properly gather and evaluate the ntlormallon sutlm1ttea. Based on my "'qui,-y- ,;;,f :ha perscn or                                                                                                 1         1
::* I --+-I --MEASUREMENT PERMrr f--REQUIREMENT SAMPLE MEASUREMENT MIT .. -.... ...
                                                                                                                                                                                                                              ~~                      ~ I -~ ~
1 l____ l-r
persons who manage the system, or those persons directly responSible for gathering the Information, the tnformaiiOn submilled IS, lo the best of my knowledge and belief, true,                                                                           423         843-7001       10     09       07 Sequoyah Site Vtce PreSident                       <UX:LI"a\e, &nd complete. Lam awar!ll that there are si_gnificant penaLttesfor submittlflQ false             SIGNATURE OF PRINCIPAL EXECUTIVE                                     .
---'-----'
_ _ _ -----~ _ _ _ _ _ ___                                 informatiOn. tncludtng the posstb*llly of f1ne and 1mpnsonment tor knowing V1olat1011s                                 OFFtcER OR AUTHORIZED AGENT                 AREA       - NUMBER-       YEA-R     MO- , DAY-
1----f---l----
_ _ _ tyPEDOR_PRINTED~-                                                                                                                                                                                              _      .       _ ____                       __
-* jNAME!TITLE PRINCIPAL EXECUTIVE OFFICER I Certi_fy under.pena!ty ottawthal and a. II*
COMMENTS AND EXPLANATION OF ANY VJOLATIONS                             !Reference all attachments her Operations performs visual inspections tor floating debris and oil and grease during all backwashes.
wef. a-praparadundermy lELEPHONE  
EPA Form 3320-1 (REV 3199)                 Previous editions may be used                                                                                                                                                                             Page 1 of 1
--f=-DATE ---*------*-------dire-cl.lon or superviSion 1n accoroaf!CU w1th a systl!lm designed to aSSl!re that qualified personnel  
 
---------* --r------r---Chnstopher R Church properly gather and evaluate the ntlormallon sutlm1ttea.
PERM1TIEE NAME/ADDRESS           (Include FacJJitv Name/l...ocatiOn if Different)                       NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)                                                           Fonn Approved.
Based on my "'qui,-y-
DISCHARGE MONITORING REPORT                     IDMR)
,;;,f :ha perscn or 1 1 persons who manage the system, or those persons directly responSible for gathering the Information, the tnformaiiOn submilled IS, lo the best of my knowledge and belief, true, 423 843-7001 10 09 07 Sequoyah Site Vtce PreSident  
!!_am<L _ TII_A~ SE()U~~_NUCL!~ ~~N!_ __ _                                                                                                                                                                                 OMS No. 2040-0004 Mm:~~$ JJJ.~QX_200CL          ___________ _
<UX:LI"a\e,  
_____ IINTE_~QE8CU6:~&SQNL _________ _
&nd complete.
_ _ _ _ S_Q_DQ_Y __:__D_8!SL IJ~
Lam awar!ll that there are si_gnificant penaLttesfor submittlflQ false SIGNATURE OF PRINCIPAL EXECUTIVE . I ___
f~ci!i~
___ _ _ ___ informatiOn.
                                -~~4_      ________ _
tncludtng the posstb*llly of f1ne and 1mpnsonment tor knowing V1olat1011s OFFtcER OR AUTHORIZED AGENT AREA -NUMBER-YEA-R MO-, DAY-___
_T_\t'A__:__S_EQYOYAHJ.J!JC'=EP.Re.LAN-T _ ~-~ __ _
_ . _ ____ __ COMMENTS AND EXPLANATION OF ANY VJOLATIONS  
[        P;R~~~~~:~R \I DISCHARQ~-!~!J R*R I ~0 r2~Tr~: (~~gt ~~ I wI lQQt!Q. __ lt8..M!1T..QJ'JSQUNIY___ --~            ____ _
!Reference all attachments her Operations performs visual inspections tor floating debris and oil and grease during all backwashes.
                                                                                                      -   nn 0 0 3 0 0 1 0 011_1\LU_I'_
EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERM1TIEE NAME/ADDRESS (Include FacJJitv Name/l...ocatiOn if Different)
_EHLUENT_IJROSS                              I       PERMIT REQUIREMENT          I                         I                         I *-*        I I     * **   * .... ~      I                I      ********
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT IDMR) !!_am<L _
I  MG/L
__ _
                                                                                                                                                                                                                        ~~CEil WEEK GRAB
___________
'SOLIDS, TOTAL SUSPENDED                             SAMPlE MEASUREMENT                                                                      -       I       ********
_ _____
19 I
_________
00530      1      0 EFFLUENT GROSS VALUE 0                            PERMIT REQUIREMENT                                                                    *-      I      *** ..****::*" :*                                           MG/L    ~-        TWICE/
_ _ _ _ _ S_Q_DQ_Y
WEEK I     GRAB SOLIDS, SETTLEABLE                                 SAMPLE MEASUREMENT                                                                      -        I      ********                  ********
__:__D_8!SL
25 00545             0 EFFLUENT GROSS VALUE 0                            PERMIT REQUIREMENT                                                                   -**      I ******:***         _.; .~ ~*-*-~~~~*-. ;*1L*~_:*-:.*:;*1.0
________
                                                                                                                                                        ** .;;;tL * *?. < < .. ; R; *:; DAILY MX J    MUL ttoNw+-GRAB MONTH FLOW, IN CONDUIT OR THRU                            SAMPLE                                                                                      **""*****                 ********           ********
_ [
MEASUREMENT                                                                      03 TREATMENT PLANT 50050    1      0 EFFLUENT GROSS VALUE 0                            PERMIT REQUIREMENT SAMPlE MEASUREMENT REPORT MOAVG
\I
                                                                                                  ,_. REPORT _
_T_\t'A__:__S_EQYOYAHJ.J!JC'=EP.Re.LAN-T
_DAILYMX MGD r                                                                                         ONCE/
_ __ _ lQQt!Q. __ lt8..M!1T..QJ'JSQUNIY
BATCH ESTIMA
___ ____ _ -nn R*R I I w I --00300100 I PERMIT I I I I I I I _EHLUENT_IJROSS 11_1\LU_I'_
                -- --                         I PERMIT REQUIREMENT SAMPLE MEASUREMENT I                         I               -
REQUIREMENT
                                                                                                                      -l                 p       '-*'!;*         ti@,J '*i~': ,I[;*t 1.-               I C--r-----
* ** * .... 'SOLIDS, TOTAL SUSPENDED SAMPlE *-* I ********  
f----1              1--~--
********  
PERMIT f--+-- ---1----
-I 00530 1 0 0 EFFLUENT GROSS VALUE SOLIDS, SETTLEABLE 00545 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 0 EFFLUENT GROSS VALUE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPlE MEASUREMENT REPORT ,_. REPORT _ MOAVG _DAILYMX
REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT L__ _ _ L___.L__ _ _ _,L__ __ _
*-I *** .. ****::*"
L_  --------
:* ********
_/
-I ********  
I NAMEilnLE PRINCIPAL EXECUTIVE OFFIC~ji::j' Cel1ify unde.r pe~l~y            of law that \his docu. men\ ancl all attachments were prepared unaer my                 /.       ".?' / /                 j     TE"lEJiHONE-     ---r---OATE .           *-1
-** I ******:***
~- --00,;-to_p_h-er_R_... C._hu_r_c_h______ ...       :~~;~~~~~s=a~=:~.=mv;!~~;::;ea;s=t~na:u;:~~~~i~~                                                          C--;;? (         ?~~                    !                 ~-*-r--1 persons who manage lhe system, or those persons d1rectly responsible for gathenng the                           S&quoyah Site Vice President                             i~ ~J
J * * .;;;tL * *? .. < < .. ; R; *:; DAILY MX **""*****  
                        .   .         .               informat*on, the 1nformat1on subm1tted is, to \he best of my knOW1Bdge and belief, true,                                                           423     843-7001         10     09       07 accurate, and complete. 1am awo~re that there a~e sigmficant penalties_ for submitting false                                                                                 ~-
********
quoyah Stte Vtce President
********
_ TYPED OR PRINTEO
03 MGD r MG/L --19 MG/L --25 MUL Fonn Approved.
                                - - - - - - - - - 1nlormat1on, 1ncludlflg the possibility Of fine and 1mpnsonment for know1ng violations SICNATURE OF PRINCIPAL EXECUTIVE. I OFFICER OR AUTHORIZED AGENT         AREA I   NUMBER rnnf. ______
OMS No. 2040-0004 GRAB WEEK I TWICE/ I GRAB WEEK ttoNw+-GRAB MONTH --*-,_ ONCE/ BATCH ESTIMA I I -l p '-*'!;* ti@,J  
YEAR - MO DAY COMMENTS AND EXPLANATION OF ANY VIOlATIONS                   /ReFerence all attachments he!
,I[;*t 1.-I C--r-----
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
----I SAMPLE -!------MEASUREMENT PERMIT REQUIREMENT f----1
EPA Form 332o:1(REv-3/9-9)-------;p:-c,ec-v-:-i<Jccuc-s-e-:d:;itio:-n-sc-m=-ay-c-be-use-:-:cd;-------------------------------------------                                                                            --=----------
-----L_ --------PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT f--+-----1----L__ __ L___.L_ ____ ,L_ __ __ _/ I NAMEilnLE PRINCIPAL EXECUTIVE Cel1ify unde. r of law that \his docu. men\ ancl all attachments were prepared unaer my /. ".?' // j TE"lEJiHONE-
Page 1 of 1
---r---OATE  
 
. *-1 --00,;-to_p_h-er_R_  
Mr Paul E. Dais Directcr State of Ter.nessee Depr:ment cf Environment and Conser;aticn Dtvision of Water Pollution Control 6th Floor, L & C Annex 401 Church Street, Nashville, TN 37243
... C._hu_r_c_h
______ ...
C--;;? ( !
persons who manage lhe system, or those persons d1rectly responsible for gathenng the S&quoyah Site Vice President . . . informat*on, the 1nformat1on subm1tted is, to \he best of my knOW1Bdge and belief, true, 423 843-7001 10 09 07 quoyah Stte Vtce President
: accurate, and complete.
1 am that there sigmficant penalties_
for submitting false SICNATURE OF PRINCIPAL EXECUTIVE.
I .-, __ .. _ *-.. ---------
1nlormat1on, 1ncludlflg the possibility Of fine and 1mpnsonment for know1ng violations OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR -MO DAY _ TYPED OR PRINTEO _ rnnf. ______ _ _ COMMENTS AND EXPLANATION OF ANY VIOlATIONS  
/ReFerence all attachments he! During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
EPA Form 332o:1(REv-3/9-9)-------;p:-c,ec-v-:-i<Jccuc-s-e-:d:;itio:-n-sc-m=-ay-c-be-use-:-:cd;-------------------------------------------
--=----------
Page 1 of 1 
*' Mr Paul E. Dais Directcr State of Ter.nessee Depr:ment cf Environment and Conser;aticn Dtvision of Water Pollution Control 6th Floor, L & C Annex 401 Church Street, Nashville, TN 37243  


==Dear Mr. Paul E. Davis:==
==Dear Mr. Paul E. Davis:==
SEQUOYAH NUCLEAR PLANT (SQN) NPDES PERMIT NO. TN0026450 WRITIEN SUBMISSION REGARDING TOWERBROM 960 RELEASE TVA is providing written notification for the subject release that occurred on August 20, 2010. The verbal notification for this same release was provided by telephone to the Chattanooga Environmental Field Office (EFO) on August 21, 2010 at 1509 and to the Tennessee Emer9ency Management Agency (TEMA) on August 21, 2010 at 1510. Since the EFO telephone notification was via voice message.
 
Stephanie Howard of TVA and Leeth a Abazid of the EFO discussed the event on August 23, 2010 Please see the Attachment (enclosed) for details of the event. If you have any questions or need information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff I certify under penally of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel property gather and evaluate the information submitted.
SEQUOYAH NUCLEAR PLANT (SQN) NPDES PERMIT NO. TN0026450 WRITIEN SUBMISSION REGARDING TOWERBROM 960 RELEASE TVA is providing written notification for the subject release that occurred on August 20, 2010.
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information.
The verbal notification for this same release was provided by telephone to the Chattanooga Environmental Field Office (EFO) on August 21, 2010 at 1509 and to the Tennessee Emer9ency Management Agency (TEMA) on August 21, 2010 at 1510. Since the EFO telephone notification was via voice message. Stephanie Howard of TVA and Leeth a Abazid of the EFO discussed the event on August 23, 2010 Please see the Attachment (enclosed) for details of the event.
the information submitted is. to the best of my knowledge and belief. true, accurate.
If you have any questions or need additio~al information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff I certify under penally of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel property gather and evaluate the information submitted. Based on my inquiry of the person or
and comprete.
*' persons who manage the system, or those persons directly responsible for gathering the information. the information submitted is. to the best of my knowledge and belief. true, accurate.
I am aware t/1at there are significant penalties for submitring false information, including the possibility of fine and imprisonment for knowing violations Sincerely,  
and comprete. I am aware t/1at there are significant penalties for submitring false information, including the possibility of fine and imprisonment for knowing violations Sincerely,                 ..,
..,
    ~17.~
crlristopher R. cr.ur:::h Site Vice President Seq,_:oyah Nudear Plant EnclosL!re cc (Erctosurei Chat:an::cga Field Divis:on cf 'v'ia:er Poi:ution State Office S*Ji:d1r.g, S:.;ite 55C US  
crlristopher R. cr.ur:::h Site Vice President Seq,_:oyah Nudear Plant EnclosL!re cc (Erctosurei Chat:an::cga E~*,:r-::nr.,e.lta\ Field O~ce Divis:on cf 'v'ia:er Poi:ution Ccnt~cl State Office S*Ji:d1r.g, S:.;ite 55C US Nwc:e3~ Re~:.:!a:Jry Co~r.:ssi-Jr
;,nN.
      ;,nN. DscL.:;;e--,~ Cor:~.~o1 Oe.s.i 1//ashir.g:::r._ 0 C 2*J555
Oe.s.i 1//ashir.g:::r._
 
0 C 2*J555 Attachment Description of the Discharge and Determination of Cause: At 1850 or: 8i20110.
Attachment Description of the Discharge and Determination of Cause:
piar:t sect.;ritJ cbser;ed a leak adjacer.t tr.e Essentiai Ra*.v Cccling 1/'/ater {ERGN) at the Plant (SON). The pumps in trar.sfer Ter.nessee R*Jer wa!er ir,to tr,e plant fer ccoling The W3S st:rafng fro!T, an cuts:de cf":er:-:ica:
At 1850 or: 8i20110. piar:t sect.;ritJ f:erscn~e: cbser;ed a leak adjacer.t ~o tr.e Essentiai Ra*.v Cccling 1/'/ater {ERGN) t:~ildir*.g at the Se(iUOJa~ N~Jdear Plant (SON). The pumps in t~is c~iidir.g trar.sfer Ter.nessee R*Jer wa!er ir,to tr,e plant fer ccoling pt..:r~cses. The lea~ W3S st:rafng fro!T, an cuts:de cf":er:-:ica: i~iect:"cr pipe fr.ttirg drair: ~ll.ig, cver.lcwing a smail t:asir. cr:to cor:crete. a.--:d o~1er a 1e~;e irtc the Ter:nes2es R:ver. Perscr.ne! w:re dis~a:cr.ed to ir.vest:s;a:e Tr.e lea{ was Cetsr~:r.ed tote trea:ed ERS'*J'.i Tr.e :r.em:ca~ used to trea: the ERC'// is Tc*11ertr:Jm 9CO. a r:-:ix~:.Jre cf scc!ium dicr,icr-:)isccyar.Lra~a ar:d scdit,;:-71 t:r:r.,:de The ~re:ir:"'.i~a~/
pipe fr.ttirg drair:
car-.:se cf the reak is the failure of tr.e drain pi~S GL;e tc corrosicn_
cver.lcwing a smail t:asir. cr:to cor:crete.
a.--:d a
irtc the Ter:nes2es R:ver. Perscr.ne!
w:re to ir.vest:s;a:e Tr.e lea{ was tote trea:ed ERS'*J'.i Tr.e used to trea: the ERC'// is Tc*11ertr:Jm 9CO. a cf scc!ium ar:d scdit,;:-71 t:r:r.,:de The car-.:se cf the reak is the failure of tr.e drain GL;e tc corrosicn_
Period of Discharge:
Period of Discharge:
The chemical feed was initiated at 1650 cr. 8,20/10 ar:d discovered at 1850 on8/20/10.
The chemical feed was initiated at 1650 cr. 8,20/10 ar:d discovered at 1850 on8/20/10. The treatment chemical feed was isolated at 1955. Therefore, the duration of the leak is bounded by a minimum of 65 minutes to a maximum of 185 minutes. A sample of water in the bottom of the catch basin was analyzed and determined to contain 55 ppm total residual chlorine. The permit limit at Outfall 101 is 0.1 ppm chlorine (total residual). No reportable quantity per CERCLA was exceeded. TDEC notification satisfied Federal insecticide, Fungicide and Rodenticide Act (FlFRA) requirements. An estimated 180 to 540 gallons of treated water was spilled. No other chemical release to the Tennessee River occurred.
The treatment chemical feed was isolated at 1955. Therefore, the duration of the leak is bounded by a minimum of 65 minutes to a maximum of 185 minutes.
Steps Being Taken to Reduce, Eliminate, and Prevent Recurrence When site emergency responders arrived at 1950 on 8/20/10, a spill response catch basin was placed under the leak location, capturing approximateiy 125 gallons. The strainer water feed was isolated at 2053. At this point, all fiow was terminated. The cause of the leak is being investigated and, once determined, permanent corrective actions will be taken. SR 236979 was written to document the incident and corrective actions. Currently ERCW chemical additions have been temporarily suspended until a full extent of condition including examination of other drain plugs can be completed. A system waik down was completed 8/21/10 to verify proper system alignmant. No misalignments were found. Metallurgical examination of the drain plug is in progress to determine the exact cause of failure.
A sample of water in the bottom of the catch basin was analyzed and determined to contain 55 ppm total residual chlorine.
The permit limit at Outfall 101 is 0.1 ppm chlorine (total residual).
No reportable quantity per CERCLA was exceeded.
TDEC notification satisfied Federal insecticide, Fungicide and Rodenticide Act (FlFRA) requirements.
An estimated 180 to 540 gallons of treated water was spilled.
No other chemical release to the Tennessee River occurred.
Steps Being Taken to Reduce, Eliminate, and Prevent Recurrence When site emergency responders arrived at 1950 on 8/20/10, a spill response catch basin was placed under the leak location, capturing approximateiy 125 gallons.
The strainer water feed was isolated at 2053. At this point, all fiow was terminated.
The cause of the leak is being investigated and, once determined, permanent corrective actions will be taken. SR 236979 was written to document the incident and corrective actions.
Currently ERCW chemical additions have been temporarily suspended until a full extent of condition including examination of other drain plugs can be completed.
A system waik down was completed 8/21/10 to verify proper system alignmant.
No misalignments were found. Metallurgical examination of the drain plug is in progress to determine the exact cause of failure.
Other Details:
Other Details:
No fish distress fish mortality, or abnormal bird migration was observed by site personnel the evening of the event and the morning following.
No fish distress fish mortality, or abnormal bird migration was observed by site personnel the evening of the event and the morning following. The afternoon of 8/21/10, TVA Biological Resource Staff visually surveyed the near shore area by boat two miles upstream ar.d four miles downstream of the Plant for any signs of dead or dying fish, cr aquatic wildlife. During the investigation, water fowl (two groups of 8 and 6 cf Canada Geese), three Great Blue Herons ar,d tNo Cormorants were observed. No dead or dyir.g fish or affected wildlife was observed in the search area. The opposite shore line was also surveyed to 1/erlf'J any effects of the biocide spill No dead or d:ting fish or affected wildlife was observed.
The afternoon of 8/21/10, TVA Biological Resource Staff visually surveyed the near shore area by boat two miles upstream ar.d four miles downstream of the Plant for any signs of dead or dying fish, cr aquatic wildlife.
 
During the investigation, water fowl (two groups of 8 and 6 cf Canada Geese), three Great Blue Herons ar,d tNo Cormorants were observed.
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT -August 2010 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Bethany Hyatte DATE: 9/8/2010 CONCURRENCES Name               R    C            Signature - Comment                      Date V    N S_ A. Howard G.J. Wynn B_ A. Wetzel I
No dead or dyir.g fish or affected wildlife was observed in the search area. The opposite shore line was also surveyed to 1/erlf'J any effects of the biocide spill No dead or d:ting fish or affected wildlife was observed.
K. Langdon tJ- tf'-1 D C. R Church X    '~7~                          U INSTRUCTIONS:       Originator will determine the review/concurrence assignment REVIEW:         Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization.
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT -August 2010 DMR ORGANIZATION:
CONCURRENCE:         Indication of agreement with the document as a whole.
Environmental DOCUMENT PREPARED BY: Bethany Hyatte DATE: 9/8/2010 Name S_ A. Howard G.J. Wynn B_ A. Wetzel K. Langdon C. R Church R C V N CONCURRENCES Signature
Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
-Comment X U Date I tJ-tf'-1 D INSTRUCTIONS:
 
Originator will determine the review/concurrence assignment REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization.
S58 101012 800- NPDES CORRESPONDENCE October 12, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1
CONCURRENCE:
6 " Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended  
: purpose, logical in construction, and clear in meaning in the eyes of the recipient.
A concurrence signature indicates that the individual would be willing to sign the document for the agency.
S58 101012 800-NPDES CORRESPONDENCE October 12, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement  
& Compliance Section 61" Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534  


==Dear Mr. Patrick Cromer:==
==Dear Mr. Patrick Cromer:==
SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR SEPTEMBER 2010 Enclosed is the September 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. On September 22, 2010 at approximately 2130 EDT, a fire occurred while placing a non-polychlorinated biphenyl (PCB) transformer in service following routine maintenance.
 
As a result of the fire, approximately 13,000 gallons of insulating oil spilled from the transformer into the surrounding gravel switchyard.
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR SEPTEMBER 2010 Enclosed is the September 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant.
The secondary containment for the switchyard is the Yard Drainage Pond. A permanent oil skimmer is located on the discharge of the Yard Drainage Pond prior to entry into the Diffuser Pond. TVA mobilized its Emergency Response Team and began recovering the spilled mineral oil. As of today, no oil has been released into Waters of U.S. (e.g., Diffuser Pond). This has been verified by both visual examination and oil and grease analysis.
On September 22, 2010 at approximately 2130 EDT, a fire occurred while placing a non-polychlorinated biphenyl (PCB) transformer in service following routine maintenance. As a result of the fire, approximately 13,000 gallons of insulating oil spilled from the transformer into the surrounding gravel switchyard. The secondary containment for the switchyard is the Yard Drainage Pond. A permanent oil skimmer is located on the discharge of the Yard Drainage Pond prior to entry into the Diffuser Pond. TVA mobilized its Emergency Response Team and began recovering the spilled mineral oil. As of today, no oil has been released into Waters of U.S. (e.g., Diffuser Pond). This has been verified by both visual examination and oil and grease analysis. Cleanup continues at both the Yard Drainage Pond and the switchyard where the spill occurred.
Cleanup continues at both the Yard Drainage Pond and the switchyard where the spill occurred.
If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete.
Sincerely,
I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
/~:?_/:/(
Sincerely, Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Envitonmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc: B. E. Brickhouse, LP 5U-C C. R. Church, OPS 4A-SQN S A. Howard, OPS 5N-SQN K. Langdon, POB 2B-SQN D. B. Nida, LP 5U-C U.S. Nuclear Regulatory Commission ATIN: Document Control Desk Washington, D.C. 20555 A. A. Ray, WT 11A-K G. R. Signer, WT 6A-K B. A. Wetzel, OPS 4A-SQN G. J. Wynn, POB 2B-SQN K. M. Hodges (EDMS), LP 2V-C Tennessee Valley Authority, P:Jst Office Sex 2000, Soddy DaJsy. Tennessee 37384-2000 October 12, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement  
Chattanooga Envitonmental Field Office               U.S. Nuclear Regulatory Commission Division of Water Pollution Control                 ATIN: Document Control Desk State Office Building, Suite 550                   Washington, D.C. 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc:
& Compliance Section 6'" Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534  
B. E. Brickhouse, LP 5U-C                           A. A. Ray, WT 11A-K C. R. Church, OPS 4A-SQN                            G. R. Signer, WT 6A-K S A. Howard, OPS 5N-SQN                            B. A. Wetzel, OPS 4A-SQN K. Langdon, POB 2B-SQN                              G. J. Wynn, POB 2B-SQN D. B. Nida, LP 5U-C                                K. M. Hodges (EDMS), LP 2V-C
 
Tennessee Valley Authority, P:Jst Office Sex 2000, Soddy DaJsy. Tennessee 37384-2000 October 12, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6'" Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534


==Dear Mr. Patrick Cromer:==
==Dear Mr. Patrick Cromer:==
SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR SEPTEMBER 2010 Enclosed is the September 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. On September 22, 2010 at approximately 2130 EDT, a fire occurred while placing a non-polychlorinated biphenyl (PCB) transformer in service following routine maintenance.
 
As a result of the fire, approximately 13,000 gallons of insulating oil spilled from the transformer into the surrounding gravel switchyard.
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR SEPTEMBER 2010 Enclosed is the September 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant.
The secondary containment for the switch yard is the Yard Drainage Pond. A permanent oil skimmer is located on the discharge of the Yard Drainage Pond prior to entry into the Diffuser Pond. TVA mobilized its Emergency Response Team and began recovering the spilled mineral oil. As of today, no oil has been released into Waters of U.S. (e.g., Diffuser Pond). This has been verified by both visual examination and oil and grease analysis.
On September 22, 2010 at approximately 2130 EDT, a fire occurred while placing a non-polychlorinated biphenyl (PCB) transformer in service following routine maintenance. As a result of the fire, approximately 13,000 gallons of insulating oil spilled from the transformer into the surrounding gravel switchyard. The secondary containment for the switch yard is the Yard Drainage Pond. A permanent oil skimmer is located on the discharge of the Yard Drainage Pond prior to entry into the Diffuser Pond. TVA mobilized its Emergency Response Team and began recovering the spilled mineral oil. As of today, no oil has been released into Waters of U.S. (e.g., Diffuser Pond). This has been verified by both visual examination and oil and grease analysis. Cleanup continues at both the Yard Drainage Pond and the switchyard where the spill occurred.
Cleanup continues at both the Yard Drainage Pond and the switchyard where the spill occurred.
If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete.
Sincerely.,                 /_
I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
  //~t:u
Sincerely.,  
~h;rR. ~h~rch Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
/_
Chattanooga Environmental Field Office                           U.S. Nuclear Regulatory Commission Division of Water Pollution Control                             ATTN: Document Control Desk State Office Building, Suite 550                                 Washington, D.C. 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013
Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
 
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) lld!lrtlli
PERMITTEE NAME/ADDRESS           (Include Facility Name/Location if Different)                         NATIONAL POLLLJTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
_p ..Q.Jt0JL2JK)O_ -----------____ IIJILTEBQEFEE.SIU&S!lNL
MAJOR                                          Form Approved DISCHARGE MONITORING REPORT                (DMR) m~--~~~~~~~~~~P~~--                                                                                                                                                                (SUBR 01)                                      OMB No_ 2040-0004 lld!lrtlli _p..Q.Jt0JL2JK)O_ -  - - -     - -     - - -       - -
_______ _ NATIONAL POLLLJTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved OMB No_ 2040-0004
____IIJILTEBQEFEE.SIU&S!lNL _______ _                                                                                                                                                F- FINAL
-* ____ SQPQY _:_D_AIS_'L_
-* ____SQPQY _:_D_AIS_'L_ TN     27~-      ______ _                                                                                                                                 DIFFUSER DISCHARGE
TN ______ _
~cm~~~~~~~~~~~m                                  ____ _                                                                                                                           EFFLUENT
____ _ !.Qta_ti_Q_
!.Qta_ti_Q_ _H_8_M_llT_Q_I\LCQY~IX- _ --* _ _ _ _ _ _ _ _
_ --* _______ _ DIFFUSER DISCHARGE EFFLUENT ATIN: Stephanie A. Howard NO DISCHARGE D ... NOTE: Read instructions before completinQ this form PARAMETER TEMPERATURE, WATER DEG. CENTIGRADE 00010 z 0 0 INSTREAM MONITORING TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 0 EFFLUENT GROSS VALUE TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 s 0 EFFLUENT GROSS VALUE PH 00400 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 00556 0 0 QUALITY OR CONCENTRATION
ATIN:   Stephanie A. Howard NO DISCHARGE               D ...
--* -* AVERAGE MAXIMUM ********
NOTE: Read instructions before completinQ this form PARAMETER                                                                                                                                     QUALITY OR CONCENTRATION                                        NO. FREQUENCY      SAMPLE
29.0 ********
                                                                                                                                                                                                    --* -*                    EX          OF        TYPE AVERAGE                MAXIMUM                UNITS                ANALYSIS TEMPERATURE, WATER DEG.                                                                                                                                               ********                29.0                                0      30 I 30    MODELD CENTIGRADE 04 00010         z   0   0                                                                                                                                             ********                30.5                DEG.C.                  SEE        CKREQ INSTREAM MONITORING TEMPERATURE, WATER DEG.
30.5
CENTIGRADE
........
                                                                                                                                                                      .... b              P~II,Y_M_lL 04          0 PERMIT 30 I 30    RCORDR 00010        1   0   0                                                                                                                                             ***"lrir***          REPORT                  OEG.C.                    SEE      CKREQ EFFLUENT GROSS VALUE                                                                                                                                                                    PAILY M_J{                                    PERMIT TEMP. DIFF. BETWEEN SAMP. &                                                                                                                                          ********                 2.2                                0      30130        CALCTD UPSTRM DEG.C 04 00016              s    0                                                                                                                                             ********                 3.0                DEG.C.               CONTIN        CALCTO EFFLUENT GROSS VALUE                                                                                                                                                                    DAILYM>L                              *----
b ***"lrir***
uous PH                                                                                                                                                                    ********               8.1                                        7 I 30      GRAB 12      Io 00400        1   0    0                                                                                                                                            "'******"'       I      9.0                   su      I          WEEKLY        GRAB EFFLUENT GROSS VALUE                                                                                                                                                                    MAXIMUM_                               ---- --*----
REPORT PAILY M_J{ ********
SOLIDS, TOTAL SUSPENDED                                                                                                                                                  7                       9                                       2130        GRAB 19      Io 00530            0    0                                                                                                                                                30                   100                   MG/L    I        MONTHLY          GRAB EFFLUENT GROSS VALUE                                                                                                                                              MOAVO                 DAILYMX                                     *----- ' - - - - -
2.2 ********
OIL AND GREASE                                                                                                                                                          <5                    <5                                    I 2130          GRAB 19 f- 0 00556            0    0 u~tMtNl        I                          I                                                                      15          I        20 MG/L
3.0 DAILYM>L
'FLOW. IN CONDUIT OR THRU TREATMENT PLANT I     SAMPI F                   ........                                   I I.
********
I MQP.Y!l_J_RA!LY                   II/IX
I 8.1 "'******"'
                                                                                                                                                                                                  -*- **-. -*--       ..    ' 0        30/30 RCORDR 50050             0   0                                                                                                                                             ********               **"'"'**"'*                                 CONTIN      RCORDR EFFLUENT GROSS VALUE                                                                                                                                                                                                                      uous NAME/TITLE PRINCIPAl EXECUTIVE OFFICER              1Certify under penalty of taw that this document and all auactunents were prepared under my             .,/**-   ~*- -~u                                    TELEPHONE            I        DATE directton or supervision tn accordance wtlh a system destgned to assUie that qualified personnel     _/ ~                ~              / . _, '                                   I      I    ----r---~
9.0 MAXIMUM_
Christopher R. Church                  property gather and evaluate the tnformation submitted. Based on mytnqutry of the person or     /   ~ L ~                c (     .--/   /
7 9 30 100 MOAVO DAILYMX <5 <5 I I 15 I 20 'FLOW. IN CONDUIT OR THRU I SAMPI F ........
                                                      'persons who manage tile system,. or those persons d~recl!y resp.onSJble tor galhenng the             Sequoyah Site Vice President information. the Information subm11ted IS , to the best of my knowledge and belief, true,                                                                 423      843-7001          10 I 10      I 07 Sequoyah Site Vice President                accurate, and complete. I am aware that there are &ignificant penalties for submittit)Q false     SIGNATURE OF PRINCIPAL EXECUTIVE 1-------=c=::=cc:c=-:::-::ccc:=c-------jinlormalion, including lhe possiblltly of line and Imprisonment for knowtng violattons.                              OFFICER OR AUTHORIZED AGENT                      AREA    I    NUMBER        I YEAR I MO I DAY I TYPED OR PRINTED                                                                                                                                                                                    L__                  L      .        .      .
I I I MQP.Y!l_J_RA!LY II/IX TREATMENT PLANT . . . -*-**-. -*--50050 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAl EXECUTIVE OFFICER Christopher R. Church Sequoyah Site Vice President
cOM_M_E_N_T_sANDEXPiANATiQN':ocF:-:AcNcY-:-:-VciO:c-LA-:::T~Io=-Ncs:---,R--:-ecfec,-e-n_c_e_acllca-cttcac--:ll-m_e_n_ts-cnce-,----------------.L------------------_L-".ll.l"-                                                - *---
********  
No closed mode operation. The following information is included in an attachment: 1. CCW data 2. veliger monitoring data EPA Form 3320*1 (REV 3199)             Previous editions may be used                                                                                                                                                               Page 1 of 2
**"'"'**"'*
 
1 Certify under penalty of taw that this document and all auactunents were prepared under my .,/**-_ directton or supervision tn accordance wtlh a system destgned to assUie that qualified personnel
DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected       Hydrocarbons             Analysis DatefTime       Analyst Method No water would come out of the pump. No sample could be obtained.
_/ /. _, ' property gather and evaluate the tnformation submitted.
CCWCHANNEL Extractable Petroleum Date/Time Collected       Hydrocarbons             Analysis Date/Time     Analyst Method 09/15/2010@ 1025             <0.10 mq/1             09/17/2010@ 0108         JOB   EPH
Based on mytnqutry of the person or / L c ( .--/ / 'persons who manage tile system,.
 
or those persons resp.onSJble tor galhenng the Sequoyah Site Vice President information.
Mean# of                               NOTES:%
the Information subm11ted IS , to the best of my knowledge and belief, true, accurate, and complete.
Mean# of             Water                           Water                SUB Sample Date          %Settlers            Sample Date Asiatic            LOCATION          Gravid Asiatic    COLLECTED BY ZM/m3             Temp. ('C)                     Temp. ('C)           LOCATION Clams/m3                                   Clam 11/03/2009   133       0         16     11/03/2009     76       16       lnplant   RCW                         CMW 11/10/2009   417     6.1       16     11/10/2009     25       16       lnplant   RCW                         CMW 11/17/2009   269       0         16     11/17/2009     0       16       lnplant   RCW                         CMW 11/2412009     36     50         15     11/2412009     18       15       lnplant   RCW                         CMW 12/01/2009     32       0       13.5     12/0112009     0     13.5     In plant   RCW                           WE 12/0812009     38       0         11     12/08/2009     0       11       In plant   RCW                         CMW 01/0512010     0       0         6     01/0512010     0       6       In plant   RCW                           B 01/12/2010     0       0         5     01/12/2010     0       5       In plant   RCW 01/19/2010     0       0         6     01/19/2010     0       6       lnplant   RCW                           p 01/26/2010     32       0         7.5     01/26/2009     0       7.5     lnplant   RCW                         NRT 02/02/2010     0       0         7     02/02/2010     0       7       lnplant   RCW                       MSW/WDT 02/09/2010     0       0         8     02/09/2010     0       8       lnplant   RCW                         BLITC 02/16/2010     0       0         5     02/16/2010     0       5       In plant   RCW                           BJ 02/23/2010   11.7     0         7     02/23/2010     0       7       lnplant   RCW                           BJ 03/02/2010     0       0         6     03/02/2010     0       6       lnplant   RCW                           PB 03/09/2010     0       0         8     03/09/2010     0       8       lnplant   RCW                         MJW 03/16/2010     0       0         10     03/16/2010     0       10       lnplant   RCW                           BC 03/23/2010     14       0         11     03/23/2010     0       11       In plant   RCW                           BC 03/30/2010     0       0         14     03/30/2010     0       14       In plant   RCW                         BAPO Apr-2010                                                                                                   no samples collected May-2010                                                                                                   no samples collected Jun-2010                                                                                                   no samples collected Jul-2010                                                                                                   no samples collected Aug-2010                                                                                                   no samples collected Sep-201 0                                                                                                 no samples collected
I am aware that there are &ignificant penalties for submittit)Q false SIGNATURE OF PRINCIPAL EXECUTIVE NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 04 0 30 I 30 MODELD DEG.C. SEE CKREQ PERMIT -----04 0 30 I 30 RCORDR OEG.C. SEE CKREQ PERMIT 04 0 30130 CALCTD DEG.C. CONTIN CALC TO uous *--------------
 
12 I o 7 I 30 GRAB su I WEEKLY GRAB ------*----19 I o 2130 GRAB MG/L I MONTHLY GRAB *-----'-----19 f-0 I 2130 GRAB MG/L -*--.. ' 0 30/30 RCORDR .... ' CONTIN RCORDR uous TELEPHONE I DATE I I 423 843-7001 10 I 10 I 07 1-------=c=::=cc:c=-:::-::ccc:=c-------jinlormalion, including lhe possiblltly of line and Imprisonment for knowtng violattons.
PERMITTEE NAME!ADDRESS                 (Include Factlify Name/Location tf Different)                                        NATIONAL POllUTANT DISCHARGE EliMINATION SYSTEM (NPDESJ                                                                                                    Form Approved MAJOR DISCHARGE MONITORING REPORT                                          (DMR)
OFFICER OR AUTHORIZED AGENT AREA I NUMBER I YEAR I MO I DAY I TYPED OR PRINTED L__ L . . .
}!a!!le__TY_A_:_ SEOIJOJ~_tiiJ~!_,E:AR I'_LANT___ _                                                                                                                                                                                           (SUBR 01)                                  OMB No. 2040-0004 Mill:~ __p_.O.J:!OX200Q _______ ~-* -*--
-*---No closed mode operation.
tlNTERQFF!C:E;_SQ.:...28.-:.SQ:NJ_ -~ _ _ _ _ __                                                                f              TN0026450                              j ~01                                  G        -~  F- FINAL
The following information is included in an attachment:
_ _ _ _SQ!J!!Y _-_DI\lSY.* IN 27~4_ _________ _
: 1. CCW data 2. veliger monitoring data EPA Form 3320*1 (REV 3199) Previous editions may be used Page 1 of 2 DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis DatefTime Analyst Method No water would come out of the pump. No sample could be obtained.
f_gctJjty_ _ _T.\LA_:_S.E:.OUOYAH NUC!.!;.AR. PbArfT __
CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 09/15/2010@
.lQ@t!Q __HAfl.!!bT_QN.J;our*u:v_ _ --* _ _ _ .- ~-* _ -*
1025 <0.10 mq/1 09/17/2010@
ATTN: stephanie A. Howard                                                                                              From
0108 JOB EPH Mean# of Water Mean# of Water SUB NOTES:% Sample Date ZM/m3 %Settlers Temp. ('C) Sample Date Asiatic Temp. ('C) LOCATION LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 11/03/2009 133 0 16 11/03/2009 76 16 lnplant RCW CMW 11/10/2009 417 6.1 16 11/10/2009 25 16 lnplant RCW CMW 11/17/2009 269 0 16 11/17/2009 0 16 lnplant RCW CMW 11/2412009 36 50 15 11/2412009 18 15 lnplant RCW CMW 12/01/2009 32 0 13.5 12/0112009 0 13.5 In plant RCW WE 12/0812009 38 0 11 12/08/2009 0 11 In plant RCW CMW 01/0512010 0 0 6 01/0512010 0 6 In plant RCW B 01/12/2010 0 0 5 01/12/2010 0 5 In plant RCW 01/19/2010 0 0 6 01/19/2010 0 6 lnplant RCW p 01/26/2010 32 0 7.5 01/26/2009 0 7.5 lnplant RCW NRT 02/02/2010 0 0 7 02/02/2010 0 7 lnplant RCW MSW/WDT 02/09/2010 0 0 8 02/09/2010 0 8 lnplant RCW BLITC 02/16/2010 0 0 5 02/16/2010 0 5 In plant RCW BJ 02/23/2010 11.7 0 7 02/23/2010 0 7 lnplant RCW BJ 03/02/2010 0 0 6 03/02/2010 0 6 lnplant RCW PB 03/09/2010 0 0 8 03/09/2010 0 8 lnplant RCW MJW 03/16/2010 0 0 10 03/16/2010 0 10 lnplant RCW BC 03/23/2010 14 0 11 03/23/2010 0 11 In plant RCW BC 03/30/2010 0 0 14 03/30/2010 0 14 In plant RCW BAPO Apr-2010 no samples collected May-2010 no samples collected Jun-2010 no samples collected Jul-2010 no samples collected Aug-2010 no samples collected Sep-201 0 no samples collected PERMITTEE NAME!ADDRESS (Include Factlify Name/Location tf Different)  
__ _p_ERMJIJ\IUM!!ER_.
}!a!!le __ TY_A_:_
b_o B9_r o;j 1
I'_LANT ___ _
AR      MQ          ~; QRIN.G_&l To    ufu~_i__3o~
__p _.O.J:!OX200Q
                                                                                                                                                                                                                ----1 J'!UM!lER.
_______ -*--tlNTERQFF!C:E;_SQ.:...28.-:.SQ:NJ_ _____ _ ____ SQ!J!!Y _-_DI\lSY
0 .       PJI DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE           [--~ ***
.* IN
NOTE: Read instructions before completinQ this form
_________
~
_ f_gctJjty_
          --pARAMETER                          --[>~r,--------:~:'::::"ERA~G::':E"'"o~iJA~NTT~IT~Y~:~~:-:L:::::A:::M::IN-G~.y-U:cN:clc:TS~r~MC'.I~N':':IM.::U::M-:-----,-0-U-A"::::C~E::YR:c::::cc:~~~E~TR::~:U:*-*- -~NIT~--~~- F~N~:~I:y S~~~~E RINE,TOTAL          RESIDUAL___ *- -* .c< *sAMPLE MEASUREMENT
_ _T.\LA_:_S.E:.OUOYAH NUC!.!;.AR.
                                                                                    ********                                 ********                       **                       ********                            o.o17                        o.o4s            ---~
PbArfT __ .lQ@t!Q __ HAfl.!!bT_QN.J;our*u:v
19
__ --* ___ .-_ -* NATIONAL POllUTANT DISCHARGE EliMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMR) f TN0026450 j G _ _ _p_ERMJIJ\IUM!!ER_.
                                                                                                                                                                                                                                                                                      -o-- 73-o* GRAB-~
J'!UM!lER.
50060        1      o    o                          -PERMIT                        "'*"'"'u"'"'                              "'***"''~~""*    ----       ****            --*"'"'"'"' 111 "'"'_-----o.~~--                                       ---**- 0.10--- MG/l                ---WEEK-         --CALCTD.
AR MQ QRIN.G_&l 0 . PJI MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE  
EFFL[JE_NT_GROSS VALUE                    _ ____      REQUIREMENT
*** Form Approved OMB No. 2040-0004 ATTN: stephanie A. Howard ----1 From b_o1B9_r o;j To NOTE: Read instructions before completinQ this form RINE,TOTAL RESIDUAL___
* _                                          MO AVG                      . INST.MAJ(__ ___                            DAYS TEMPERATURE- C, RATE OF                                    SAMPlE                    ********                                           Q                                              ********                         ********                                             ..      Q        30 I 30  CALCTD CHANGE                                                MEASUREMENT                                                                                          62
*--* .c< *sAMPLE ********
                                                      --====c---J------,---,-+------ -                                                                                     ----                                                                 .. --           --------*         1---t-:.:-=:cc-i-=*:-:
********
82234        1      0      0                              PERMIT                   "'*"'"'*"'**                                       2                OEG                          *"'""""""**                       .,...,.,...,,.,..,              ** 111 ****           Uh              CONTIN    CALCTO EFFLUENT GROSS VALUE                                    REQUIREMENT                                                I* DAILY MX                            C/HR                                                                                                                              I . UOUS
** ********
  ----------------*-**-----                               SAMPLE                                                                                                                                                              -                                                   !--* ***------!----
o.o17 o.o4s
MEASUREMENT PERMIT                                                 I
-o---37-73-o*
                                                                                                                                                                          ~---*-*----~
MEASUREMENT 19 50060 1 o o -PERMIT "'*"'"'u"'"'
                                                                                                                                                                                                                --                                       -----                       . - ---+--:--
----****
__      ___ -----*****           REQUIREMENT                ._            **                             ,      . * .        .                              *         .                                                                                      __
---**-0.10---MG/l ---WEEK---CALCTD.
SAMPLE                                                                                                                                                                                                                        --jf----+---
EFFL[JE_NT_GROSS VALUE _ ____ REQUIREMENT
MEASUREMENT I--PERMIT___                                              *- .                               :.                                           *.               -, ..               ****-.---- - - - - - -                                                       *-*--
* _ MO AVG . INST.MAJ(__
          -------*                                    REQUIREMENT                                           ._...     .*..                    ..**                           :*                              -. ***                ----                                                 .*
___ DAYS TEMPERATURE-C, RATE OF SAMPlE ********
SAMPLE MEASUREMENT PERMIT                                  *                                                                        ---*:***-* - - - - - - - - - - - - - - - - - -                                                           - - - - - * * * -*-****---
Q ********  
    - _ __                                              REQUIREMENT                                                            *.. **                                    _          .* ..            ..    *.                                      . .                                            ___ ***-****----*-*
********  
SAMPLE MEASUREMENT PERMIT                                                    *-- .... ***- ------                                 fe--------r---                                                                         -----                                             -
.. Q 30 I 30 CALCTD CHANGE MEASUREMENT 62 --====c---J------,---,-+------
REQUIREMENT                                     * >          ** *...                                                . : *                *.        .* I ***
-----.. ----------*
1-----------*-                                              SAMPLE                                                                        -----f------1~---~+---~---1-----+---f--                                                                                                              -- - * - - - - - -
1---t-:.:-=:cc-i-=*:-:
MEASUREMENT PERMIT            ----------c-*---------                                                                                                                                            . -----.--                          -----**-----*-------
82234 1 0 0 PERMIT "'*"'"'*"'**
                    - ------------- __                  REQUIREMENT            ---------                                                        *. _____            ,_.c.**-~_                  _:__c!                                                              ~-----~-----'-----
2 OEG *"'""""""**  
                    ~!*cii'*''"''"'
.,...,.,...,,.,..,
                            -
**111**** Uh CONTIN CALCTO EFFLUENT GROSS VALUE REQUIREMENT I* DAILY MX C/HR I . UOUS ----------------*-**-----
* iiffiOT"' ""'....
SAMPLE -!--* ***------!----
d1rect1on or swperv1s1on  ~ .,;. ;I
MEASUREMENT
                                                                                    <.rm accordance hat..I. his do_'".
----*--*-
w1thment a systemand all  designed anachments to assure w_erethat    ..,_re~
-------. ----+--:--
: p. ,
PERMIT I . . :* __ _ __ -----*****
* qualifiedunder personnel my                            ~
REQUIREMENT
                                                                                                                                                                                                                              *         -::-z-;~_.7~;>-/--~TELEPHONE-n
. _ ** , . * . . * . __ SAMPLE --jf----+---
                                                                                                                                                                                                                                        ///:.C-;;f>?/7 -------                                 T* nDATe-;
MEASUREMENT I--PERMIT___
k                                                                                                                                                                                                              ~>u*~~-*ro~~ --~t'"""''_bdw>10.i Christopher R. Church                      properly gather and evaluate the 1nformallon submllled Based on my InqUiry of the person or persons who manage the system, or lhosa persons directly respons1ble for gathen11g the 1nformat1on, the mformallon subm1lled 1S , to lhe best of my knowledge and belief, true.                                                     __    Seq~oyah Site Vice Pn:_~i~~-~~----*-                        423    843-7001      I 10    10        07 Sequoyah Site V1ce President                    accurate. and complete 1am aware that there are s1gmf1cant penalties for subm1tt1ng false                                                    SIGNATURE OF PRINCIPAl EXECUTIVE
*-. :. *. -, .. ****-.----
      .-.-*---------------*----                             InformatiOn. 1nclud1ng the poss1b1hty of fme and 11nprlsonmen1 for knowmg Vlofa11Dns TYPED OR PRINTED
------*-*---------*
                  --~--*-*--    -*--- -*~-----
REQUIREMENT
COMMENTS AND EXPLANATION OF ANY VIOLATIONS                          tReference all attachments her The following injections occurred: 1. Floguard MS6236 (max. calc. cone. was 0.038mg/L-~Iimit 0.2mgll) 2. Spectrus CT1300 (max. calc. cone. was 0.040mgll--limit 0.050mg/L) 3. Spectrus CT1300 (low detection level analytical method was <0.050mg/l--limit 0.050mg/L)
._... .*.. ..** :* -. *** ----.* SAMPLE MEASUREMENT PERMIT * ---*:***-*
          -------~
----------
t:t"A t"orrn .J320*1 {REV 3199)               Previous editions may be used                                                                                                                                                                                                                Page 2 of 2
-------------***  
 
-*-****---
PERMITTEE NAME/ADDRESS                          (Include Facilitv Name/Location if Different}                                NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)                                                                                                        Form Approved.
-_ __ REQUIREMENT
MAJOR DISCHARGE MONITORING REPORT                            (DMR)
*.. ** _ .* .. .. *. . . ___ ***-****----*-*
!LamL _ "fl!.A.: s~:quov~H_I'jUCLE~R I'LANT___ _                                                                                                                                                                                          (SUBR 01)                                          OMB No_ 2040-0004 P._9Qr~~:? ___ P,Q. __6QX_2_@Q_ ___________________ _
SAMPLE MEASUREMENT PERMIT *--.... ***-------fe--------r---
____(L~J<.~Qf~!CUil."85QNL __________ _
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SQPI~Y .* Pl\JSY, TN .~?~!l4__ _
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TN0026450                -l ffRMlT.miMBfR____ LJ:liSQiARG.E.N!!M6ER 101 Q                            F- FINAL D(FFUSER DISCHARGE fiK!!i\Y.. _TY11.:.5EQI.!QYI\IiJillCLfA.R~l..AJIIT _ _ *-
SAMPLE  
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EFFLUENT
. -----.-------**-----*-------
!Qc_atjQ___HAMUJQN.CQUN.TY ATTN:    Stephanie A. Howard
--------------
----PARAMETER                                              *k~                          . * -. ..            OUANT!TY OR LOADING                                  ,---*-
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QUALITY OR CONCENTRATION NO DISCHARGE r~MAXIMUM
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NO. FREQUENCY SAMPLE EX            OF ANAlYSIS TYPE
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* under my -* d1rect1on or swperv1s1on m accordance w1th a system designed to assure that qualified personnel Christopher R. Church properly gather and evaluate the 1nformallon submllled Based on my InqUiry of the person or persons who manage the system, or lhosa persons directly respons1ble for gathen11g the . . . 1nformat1on, the mformallon subm1lled 1S , to lhe best of my knowledge and belief, true. Sequoyah Site V1ce President accurate.
UNITS          MINIMUM                              AVERAGE                                                      UNITS feO-RON, r6TAL________ --------                                       sAMP-LE--
and complete 1 am aware that there are s1gmf1cant penalties for subm1tt1ng false .-.-*---------------*----
MEASUREMENT
InformatiOn.
                                                                                                                                                              ..                                                  <0.20                                                      19 0
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-------. .. . ... __
-----~------------~-             --** *~-* -----------                                                                                                   ~-         --                                                                                                     ------ --~---.
Site Vice 423 843-7001 I 10 10 07 SIGNATURE OF PRINCIPAl EXECUTIVE COMMENTS AND EXPLANATION OF ANY VIOLATIONS tReference all attachments her The following injections occurred:
SAMPLE MEASUREMENT
: 1. Floguard MS6236 (max. calc. cone. was 0.2mgll)
                                                              ---;;* *------ -------,---------,-----:-- ----. -,--       -- ----------------                                                                                    - - -------                  - -  ~-                        *-  - - - - - r--*--**
: 2. Spectrus CT1300 (max. calc. cone. was 0.040mgll--limit 0.050mg/L)
PERMIT REQUIREMENT I             .                                                                               *--
: 3. Spectrus CT1300 (low detection level analytical method was <0.050mg/l--limit 0.050mg/L) t:t"A t"orrn .J320*1 {REV 3199) Previous editions may be used Page 2 of 2 PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different}
SAMPLE MEASUREMENT I'
!LamL _ "fl!.A.:
                                                                                                                                          ..                                                                                                     --**-~--c--
I'LANT ___ _
PERMIT                                                                                                            ***  **
___ P,Q. __ 6QX_2_@Q_
REQUIREMENT                                                      .                                               . ** ..        . ..     .* .. *                                        .*
___________________
I-* .......      ***--------~
_ ____
SAMPLE MEASUREMENT PERMIT                                     .**
__________
1-*-.- .. - . - - -                          --------- ----                   1- .           ---~            . - **----*-                                                 ..
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REQUIREMENT                                   .      I        *:          . :.                 ,.: *. *.*...                      1**.:::._.                              I SAMPLE MEASUREMENT PERMIT
.* Pl\JSY, TN
                                                                                                                                                                      ~
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___ HAMUJQN.CQUN.TY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) 101 Q -l TN0026450 ffRMlT.miMBfR____
                                                                                                                                                                                                                                                                                                                        -~
LJ:liSQiARG.E.N!!M6ER MAJOR (SUBR 01) F-FINAL D(FFUSER DISCHARGE EFFLUENT NO DISCHARGE  
REQUIREMENT                                                            ...*.*                                .                   ..
[J *** Form Approved.
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OMB No_ 2040-0004 NOTE: Read instructions before completm!=l this form ----PARAMETER .. ***-. . .. OUANT!TY OR LOADING ,---*-------
SAMPLE MEASUREMENT
........
                                                              --PERMoT*--
-------* -*---.--.. __l_ QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE UNITS MINIMUM AVERAGE UNITS ANAlYSIS feO-RON, r6TAL ________
                                                                                          ---~-..,--~------   __,__ "".    --*--.-*-           ---*- ---                                                                                         --~-*----                                      - - - - ------
--------sAMP-LE--
REQUIREMENT                          . .                        ..                                              .                  I                              ..
--.. -----*-----------
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-------01022 1 0 0 PERMIT **** oll)illr11
SAMPLE MEASUREMENT PERMIT REQUIREMENT
"'***"'***
                                                                                                                                                                                              .z;- #:~1-
*"'** ******* REPORT **"'****
                                                                                                                                                                                                                                                                          ~----~--
MG/L QTRLY GRAB EFFLUENT GROSS VALUE REQUIREMENT
NAME/TITLE :PoROICNCC::CIPCACL:-EXEC_U_T_IV_E_o_Ff=ICER Christopher R. Church I Cert1fy under penally of law that this documem and all altachments were prepared under my d1rect10n or superviSIOn In accordance w1th a system des1gne<:1 to assure that qualified personnel properly gather and evaluate the intormat1on submitted Based on my inqu1ry of tt1e person or p~rson~ who manage llle syslem, or !hose persons dnectly respons1ble lor gathenng the
--**
_TELEPfjONE      ***;1~-~~~::.:?:x.:_E~..::    ..
-------------------
Pres~ent___
SAMPLE MEASUREMENT  
                                                                                                                                                                                                                                                                        --j----- ~0 1
---;;* *-------------,---------,-----:--
Sequoyah Site V1ce President t=-----TY*P*E--~~-~~~-T_E_D
----. -,--------------------
                                            .         .                1nformalion, the 1nlonnallon submttted IS, lo the best of my knowledge end belief, true, accurate, and complete_ I am aware that !here are s1gn1ficant penalties lor subm1t1ing false poss1~~~: :~=:~~~~::i~:~=~~~~~r know1ng violations.
-----------*------r--*--**
1-1 Sequo_yah Site Vice
PERMIT REQUIREMENT I . *--SAMPLE MEASUREMENT PERMIT .. *** ** -,---,-------;---*
                                                                                                                                                                                          --;:S;;;IG:CNCCAT;;U;;;RE OF PRINCIPAL EXECUTIVE
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                                                                                                                                                                                                                                                                        .423      843-7001
;--** REQUIREMENT
                                                                                                                                                                                                                                                                                                      -*- *- * **10        07- _
. . ** .. . .. .* .. * .* I-* .......
1nformat1on, Including lhe OFFIC~~~~~-~~~~~IZ~~-~~-~- -~~~ ~-~~~R                                                          YEAR       MD       DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                                     tReferencea/lattachments her Boron was sampled on 717110.
SAMPLE MEASUREMENT
                -*--** *-****----*-** --    ~-------~-
. ** 1-*-. -.. -.------------
EPA Form 3320-1 (REV 3199)                             Previous editions may be used                                                                                                                                                                                                           Page 1 of 1
----1-.
 
. -**----*-.. PERMIT I *: . :. .* REQUIREMENT
PERMIITEE NAME/ADDRESS               (Include Facility Name/Location if Different)                             NATIONAl POllUTANT DISCHARGE EliMINATION SYSTEM (NPDESJ                                                               Form Approved.
. ,.: *. *.* ... 1**.:::._.
MAJOR DISCHARGE MONITORING REPORT                (DMRJ
I SAMPLE MEASUREMENT . --*--------------
~~--~~~~ov~~uc~~~~~---                                                                                                                                                                    (SUBR 01)                                   OMB No_ 2040-0004 Mdress _p_,_Q._BOX.:;:ggo______________ _
. r----PERMIT . REQUIREMENT
---~m~~~ES~~~L                                ______ _                                                           I          TN0026450                                  101 T            F- FINAL
... *.* .. . I . . ------**
---~ID~M~rn~~L                                ______ _                                                                                                                                   BIOMONITORING FOR OUTFALL 101 fill;;.i.!.i(Y__T~_:__SEQUQ'ffilf_l..IUCLEA_R_el_A.N_T_ _ _ _ _
**-SAMPLE MEASUREMENT
EFFLUENT
--PERMoT*--
~~~-~~m~~=----------
__ , __ "". --*--.-*-
                                                                                                                                                                                            *** NO DISCHARGE            D ...
---*----
ATTN: stephanie A. Howard
r-------------REQUIREMENT  
                                                                                                                                                                          -   I  "=--"            NOTE: Read instructions before completint:~ this form.
. . .. I . . . -SAMPLE MEASUREMENT  
                                                  ~
---*** ----** *---*-** ....... **-----------
PARAMETER                                                                      QUANTITY OR LOADING                                                  QUALITY OR CONCENTRATION                                  NO. FREQUENCY SAMPLE EX            OF          TYPE AVERAGE                      MAXIMUM              UNITS              MINIMUM            AVERAGE                                  UNITS                    ANALYSIS MAXIMUM IC25 STATRE 7DAY CHR
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                                                                                    ********                      ...........            -            Monitoring            ********
PERMIT REQUIREMENT
23 CERIODAPHNIA TRP3B            1    0    0 MEASUREMENT PERMIT                      ........                    *-*****                ....        Not Required 45.2            ********                ********      PERCENT                      SEE      COMPOS REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                                  MINIMUM                                                                                PERMIT IC25 STATRE 7DAY CHR                                    SAMPLE                      ********                      ********                            Monitoring            ******"'*                    ,.,
.*. --
MEASUREMENT                                                                                                                                                          23 PIMEPHALES                                                                                                                                          Not Requir~d TRP6C            1 EFFLUENT GROSS VALUE 0     0                           PERMIT REQUIREMENT
----*-*--' I NAME/TITLE
                                                                                    ********                     ""**"'****           *-*                 45.2
:PoROICNCC::CIPCACL:-EXEC_U_T_IV_E_o_Ff=ICER I Cert1fy under penally of law that this documem and all altachments were prepared under my -----d1rect10n or superviSIOn In accordance w1th a system des1gne<:1 to assure that qualified personnel Christopher R. Church properly gather and evaluate the intormat1on submitted Based on my inqu1ry of tt1e person or who manage llle syslem, or !hose persons dnectly respons1ble lor gathenng the . . . 1nformalion, the 1nlonnallon submttted IS, lo the best of my knowledge end belief, true, Sequoyah Site V1ce President
: accurate, and complete_
I am aware that !here are s1gn1ficant penalties lor subm1t1ing false 1nformat1on, Including lhe know1ng violations.  
. z;-
_TELEPfjONE
.. Sequo_yah Site Vice  
.423 843-7001 1 10 07-_ 1-1 --;:S;;;IG:CNCCAT;;U;;;RE OF PRINCIPAL EXECUTIVE  
--j------*-*-* *
* YEAR MD DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS tReferencea/lattachments her Boron was sampled on 717110. -*--** *-****----*-**  
--
EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMIITEE NAME/ADDRESS (Include Facility Name/Location if Different)
NATIONAl POllUTANT DISCHARGE EliMINATION SYSTEM (NPDESJ MAJOR (SUBR 01) F-FINAL Form Approved.
OMB No_ 2040-0004 DISCHARGE MONITORING REPORT (DMRJ Mdress _p _,_Q._BOX.:;:ggo
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______ _ I TN0026450 fill;;.i.!.i(Y
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ATTN: stephanie A. Howard ' *-' --' -* ' --*-PARAMETER QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM ----------
IC25 STATRE 7DAY CHR ********  
...........
Monitoring MEASUREMENT
-CERIODAPHNIA Not Required TRP3B 1 0 0 PERMIT ........
*-***** .... 45.2 EFFLUENT GROSS VALUE REQUIREMENT MINIMUM IC25 STATRE 7DAY CHR SAMPLE ********
********
Monitoring
-PIMEPHALES MEASUREMENT Not TRP6C 1 0 0 PERMIT ********  
""**"'****  
*-* 45.2 EFFLUENT GROSS VALUE REQUIREMENT
_IIIIJMINUM._
_IIIIJMINUM._
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT I--REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT PRINCIPAL EXECUTIVE OFFICER Christopher R. Church 1 Certify under penally of law that this document and all attachments were prepared under my direction or in accotdance w1th a system des1gned to assure that qualified personnel properly gather and evaluate the information submitled.
                                                                                                                                                                              ********                ********      PERCENT                      SEE PERMIT COMPOS
Based Oil my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the 1nformat1on, the information submiHed 1s , to the best of my knowledge and behef, true, 101 T BIOMONITORING FOR OUTFALL 101 EFFLUENT
                                                                                                                                                                                                                --                            --------~-
*** NO DISCHARGE D ... -I "=--" NOTE: Read instructions before this form. ----**--*
                                                                                                                                                                                                                                                            ---~------
QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT I--
********
REQUIREMENT
........
                                                                                                                                                                                                                                          ---  ---------    -~----
23 * ******* ********
SAMPLE MEASUREMENT PERMIT REQUIREMENT
PERCENT SEE COMPOS PERMIT -------------. *-*. ----------*------
                                                                                                                                                                                                                                      **-*      -------      -        --~-
----******"'*
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT L__
.... ,., ... 23 ********
N_AME/TIT~E PRINCIPAL EXECUTIVE OFFICER              1 Certify under penally of law that this document and all attachments were prepared under my                                                                  TELEPHONE                        DATE direction or supen~is1on in accotdance w1th a system des1gned to assure that qualified personnel Christopher R. Church                  properly gather and evaluate the information submitled. Based Oil my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the 1nformat1on, the information submiHed 1s , to the best of my knowledge and behef, true,                                                                  423      843*7001              10    10      07 Sequoyah Site Vice President                accurate. and complete. 1 am aware that there <Ire significant penalties for submitting false            SIGNATURE OF PRINCIPAL EXECUTIVE f                                                        finfor.-nation. including /he passil:>ili!y of fine and imprisonment for knowing Yiolatioos_
********
OFFICER OR AUTHORIZED AGENT                            NUMBER              YEAR    MO    DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS                          (Reference all attachments het Toxicity was not sampled in September 2010.
PERCENT SEE COMPOS PERMIT --
EPA Form 3320-1 (REV 3199)                  Previous editions may be used                                                                                                                                                                  Page 1 of 1
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(Include Facility Name/Location if DifferenfJ                            NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ PERMITIEE NAME/ADDRESS                                                                                                                                                                                      MAJOR                                            Form Approved
**-* ------------**---
---------------L__ TELEPHONE DATE 423 843*7001 10 10 07 Sequoyah Site Vice President accurate.
and complete.
1 am aware that there <Ire significant penalties for submitting false f finfor.-nation.
including
/he passil:>ili!y of fine and


==Dear Mr. Patrick Cromer:==
==Dear Mr. Patrick Cromer:==
SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR OCTOBER 2010 Enclosed is the October 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Daily sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 10/26/2010 have all yielded results below detection limits for oil and grease. On 10/1/2010 the permit limit for pH at Outfall103 (discharge from the Low Volume Waste Treatment Pond-LVWTP) was exceeded.
 
The pH sampled at 1800 EST on 10/1/2010 was 9.2. The permit limit is 6.0 to 9.0. A sample of Outfall1 01 (Diffuser Pond Discharge to the Tennessee River) was collected at 2116 EST to verify discharge to the river did not exceed limits. The pH of Outfall 101 was within limits at 7.9 pH units. On 10/1/2010 at 2300 EST the pH of Outfall 103 was 9.0 and determined to be back within permit limits. The suspected cause of the exceedances at Outfall 103 was a high pH discharge from the High Crud Tank of the condensate demineralizer system. The pH of the High Crud Tank was approximately 9.0 at the time of discharge to the Turbine Building Sump which discharges to the Low Volume Waste Treatment Pond. The event was documented in the site's corrective action program.
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR OCTOBER 2010 Enclosed is the October 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant.
Corrective action to date includes the revision of the condensate demineralizer effluent procedure to change the acceptable effluent pH range from 6.0 to 9.0 to 6.0 to 8.0. A study has also been initiated to determine if an engineered pH control system to the L VWTP is a viable option. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
Daily sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 10/26/2010 have all yielded results below detection limits for oil and grease.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.
On 10/1/2010 the permit limit for pH at Outfall103 (discharge from the Low Volume Waste Treatment Pond- LVWTP) was exceeded. The pH sampled at 1800 EST on 10/1/2010 was 9.2.
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information
The permit limit is 6.0 to 9.0. A sample of Outfall1 01 (Diffuser Pond Discharge to the Tennessee River) was collected at 2116 EST to verify discharge to the river did not exceed limits. The pH of Outfall 101 was within limits at 7.9 pH units. On 10/1/2010 at 2300 EST the pH of Outfall 103 was 9.0 and determined to be back within permit limits.
, including the possibility of fine and imprisonment for knowing violations.
The suspected cause of the exceedances at Outfall 103 was a high pH discharge from the High Crud Tank of the condensate demineralizer system. The pH of the High Crud Tank was approximately 9.0 at the time of discharge to the Turbine Building Sump which discharges to the Low Volume Waste Treatment Pond. The event was documented in the site's corrective action program. Corrective action to date includes the revision of the condensate demineralizer effluent procedure to change the acceptable effluent pH range from 6.0 to 9.0 to 6.0 to 8.0. A study has also been initiated to determine if an engineered pH control system to the LVWTP is a viable option.
If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
 
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
~el~//_
~-~'
Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc: B. E. Brickhouse, LP 5U-C C. R. Church, OPS 4A-SQN S. A. Howard, OPS 5N-SQN K. Langdon, POB 2B-SQN D. B. Nida, LP 5U-C U.S. Nuclear Regulatory Commission ATIN: Document Control Desk Washington, D.C. 20555 A. A. Ray, WT 11A-K G. R. Signer, WT 6A-K B. A. Wetzel, OPS 4A-SQN G. J. Wynn, POB 2B-SQN K. M. Hodges (EDMS), LP 2V-C Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 November 10, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement  
Chattanooga Environmental Field Office             U.S. Nuclear Regulatory Commission Division of Water Pollution Control                 ATIN: Document Control Desk State Office Building, Suite 550                   Washington, D.C. 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc:
& Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534  
B. E. Brickhouse, LP 5U-C                           A. A. Ray, WT 11A-K C. R. Church, OPS 4A-SQN                           G. R. Signer, WT 6A-K S. A . Howard, OPS 5N-SQN                           B. A. Wetzel, OPS 4A-SQN K. Langdon, POB 2B-SQN                             G. J. Wynn, POB 2B-SQN D. B. Nida, LP 5U-C                                K. M. Hodges (EDMS), LP 2V-C
 
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 November 10, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534


==Dear Mr. Patrick Cromer:==
==Dear Mr. Patrick Cromer:==
SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR OCTOBER 2010 Enclosed is the October 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Daily sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 10/26/2010 have all yielded results below detection limits for oil and grease. On 10/1/2010 the permit limit for pH at Outfall103 (discharge from the Low Volume Waste Treatment Pond-LVWTP) was exceeded.
 
The pH sampled at 1800 EST on 10/1/2010 was 9.2. The permit limit is 6.0 to 9.0. A sample of Outfall 101 (Diffuser Pond Discharge to the Tennessee River) was collected at 2116 EST to verify discharge to the river did not exceed limits. The pH of Outfall101 was within limits at 7.9 pH units. On 10/1/2010 at 2300 EST the pH of Outfall103 was 9.0 and determined to be back within permit limits. The suspected cause of the exceedances at Outfall 103 was a high pH discharge from the High Crud Tank of the condensate demineralizer system. The pH of the High Crud Tank was approximately 9.0 at the time of discharge to the Turbine Building Sump which discharges to the Low Volume Waste Treatment Pond. The event was documented in the site's corrective action program.
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR OCTOBER 2010 Enclosed is the October 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant.
Corrective action to date includes the revision of the condensate demineralizer effluent procedure to change the acceptable effluent pH range from 6.0 to 9.0 to 6.0 to 8.0. A study has also been initiated to determine if an engineered pH control system to the LVWTP is a viable option. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
Daily sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 10/26/2010 have all yielded results below detection limits for oil and grease.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.
On 10/1/2010 the permit limit for pH at Outfall103 (discharge from the Low Volume Waste Treatment Pond- LVWTP) was exceeded. The pH sampled at 1800 EST on 10/1/2010 was 9.2.
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for
The permit limit is 6.0 to 9.0. A sample of Outfall 101 (Diffuser Pond Discharge to the Tennessee River) was collected at 2116 EST to verify discharge to the river did not exceed limits. The pH of Outfall101 was within limits at 7.9 pH units. On 10/1/2010 at 2300 EST the pH of Outfall103 was 9.0 and determined to be back within permit limits.
The suspected cause of the exceedances at Outfall 103 was a high pH discharge from the High Crud Tank of the condensate demineralizer system. The pH of the High Crud Tank was approximately 9.0 at


==Dear Mr. Patrick Cromer:==
==Dear Mr. Patrick Cromer:==
SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR NOVEMBER 2010 Enclosed is the November 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Also, enclosed is the revision to the Discharge Number 101 T, Biomonitoring for Outfall 101 for October 2010. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 11/29/2010 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.
 
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete.
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR NOVEMBER 2010 Enclosed is the November 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Also, enclosed is the revision to the Discharge Number 101 T, Biomonitoring for Outfall 101 for October 2010. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 11/29/2010 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sincerely, r1 Michael D. Ska Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Sincerely, r1       t;~,
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc: B. E. Brickhouse, LP 5U-C D. A. Day, POB 2B-SQN S. A. Howard, OPS 5N-SQN K. Langdon, POB 2B-SQN D. B. Nida, LP 5U-C U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, DC 20555 A. A. Ray, WT 11A-K G. R. Signer, WT 6A-K M. D. Skaggs, OPS 4A-SQN B. A. Wetzel, OPS 4A-SQN K. M. Hodges (EDMS), LP 2V-C PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Ad!ttess
Michael D. Ska Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
_P _,Q._!i_OX2J!!XL
Chattanooga Environmental Field Office                   U.S. Nuclear Regulatory Commission Division of Water Pollution Control                     ATTN: Document Control Desk State Office Building, Suite 550                       Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc:
__________ _
B. E. Brickhouse, LP 5U-C                               A. A. Ray, WT 11A-K D. A. Day, POB 2B-SQN                                   G. R. Signer, WT 6A-K S. A. Howard, OPS 5N-SQN                                M. D. Skaggs, OPS 4A-SQN K. Langdon, POB 2B-SQN                                  B. A. Wetzel, OPS 4A-SQN D. B. Nida, LP 5U-C                                     K. M. Hodges (EDMS), LP 2V-C
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004
 
____ _ I TN0026450  
PERMITTEE NAME/ADDRESS          (Include Facility Name/Location if Different)                          NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)                                                        Form Approved.
\ \ 101 T \ PERMIT NUMBER DISCHARGE NUMBER I YfARI MO FJ"'"" Fl MO I ""' I BIOMONITORING FOR OUTFALL 101 EFFLUENT ATTN: stephanie A. Howard From 10 10 01 To 10 10 31 *** NO DISCHARGE D ... NOTE: Read instructions before completin!l this form. PARAMETER OUANTrrY OR LOADING QUALrrY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ********  
MAJOR DISCHARGE MONITORING REPORT                (OMR) m~--N~ SEOUOYA~UCLEARP~~ ---                                                                                                                                                 (SUBR 01)                               OMB No. 2040-0004 Ad!ttess _P_,Q._!i_OX2J!!XL _ _ _ _ _ _ _ _ _ _ _
********  
- --~lmER~~UB-2A - SO~ -------
>100.0 ********  
---~OD~~M~W3~- ---- ---
********
I          TN0026450 PERMIT NUMBER
0 1/180 COMPOS ... 23 CERIODAPHNIA MEASUREMENT ITRP3B 1 0 0 PERMIT ********
                                                                                                                                                \ \           101 T DISCHARGE NUMBER
********
                                                                                                                                                                            \
..... 45.2 ********  
F- FINAL BIOMONITORING FOR OUTFALL 101
********
~~~-~~~~~~~lE~P~ID____                                          _
PERCENT SEE COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM PERMIT IC25 STATRE 7DAY CHR SAMPLE ********  
10 I 10 FJ"'""                  To Fl EFFLUENT Loc~~~AMIU~~~~--- ---- - - -
********  
ATTN: stephanie A. Howard                                                                          From    IYfAR        MO        01                10    MO    31 I 10 I ""'
>100.0 ********  
                                                                                                                                                                                *** NO DISCHARGE           D ...
********
NOTE: Read instructions before completin!l this form.
0 1/180 COMPOS ... 23 PIMEPHALES MEASUREMENT TRP6C 1 0 0 PERMIT ********
                                              ~
********
PARAMETER                                                                 OUANTrrY OR LOADING                                                   QUALrrY OR CONCENTRATION                           NO. FREQUENCY       SAMPLE EX       OF           TYPE AVERAGE                   MAXIMUM                 UNITS           MINIMUM             AVERAGE         MAXIMUM             UNITS             ANALYSIS I C25 STATRE 7DAY CHR C ERIODAPHNIA SAMPLE MEASUREMENT
.... 45.2 ********  
                                                                              ********                 ********                 ...            >100.0             ********         ********           23        0     1/180       COMPOS ITRP3B     1   0     0                           PERMIT
........
                                                                                                                                .....             45.2             ********         ********       PERCENT                 SEE       COMPOS REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                         MINIMUM                                                                     PERMIT IC25 STATRE 7DAY CHR                             SAMPLE                     ********                 ********                 ...              >100.0           ********         ********           23        0     1/180       COMPOS PIMEPHALES TRP6C     1     0   0 MEASUREMENT PERMIT REQUIREMENT
PERCENT SEE COMPOS EFFLUENT GROSS VALUE REQUIREMENT MIMINUM PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT  
                                                                                                                                ....               45.2             ********         ........         PERCENT               SEE       COMPOS EFFLUENT GROSS VALUE                                                                                                                         MIMINUM                                                                     PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
-NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penalty or law that this document and all allachments were prepared under my TELEPHONE DATE direction Of supervision In I!CCOfdance wllh a system designed to assute that qualifoed personnel Michael D. Skaggs gather and evaluate the information submitted.
                                                                                                                                                          ~"l~~,;deol NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penalty or law that this document and all allachments were prepared under my                                                                 TELEPHONE                     DATE direction Of supervision In I!CCOfdance wllh a system designed to assute that qualifoed personnel Michael D . Skaggs                   prope~y gather and evaluate the information submitted. Based on my inquiry or the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate,                                           423      843-7001          10      12    08 Sequoyah Site Vice President               and co.-nplete I am aware lhatlhere are significant penalties fo.- submilling false information.     SIGNATURE OF PRINCIPAL EXECUTIVE               I TYPED OR PRINTED including the possibility of fine and imprisonment fo.- knowing violations OFFICER OR AUTHORIZED AGENT           AREA CODF l   NUMBER         YEAR     MO   DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                 (Reference all attiJchments here Toxicity sampling began on October 31 and ended on November 5. This is the revised October 2010 DMR for 101T. Toxicity report is included.
Based on my inquiry or the person or persons who manage the system, or those persons directly responsible for gathering the 423 843-7001 10 12 08 information
EPA Fonn 3320-1 (REV 3/99)             Previous editions may be used Page 1 of 1
, the information submitted is , to the best of my knowledge and belief, true, accurate, Sequoyah Site Vice President and co.-nplete I am aware lhatlhere are significant penalties fo.-submilling false information.
 
SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment fo.-knowing violations OFFICER OR AUTHORIZED AGENT AREA l NUMBER YEAR MO DAY TYPED OR PRINTED CODF COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attiJchments here Toxicity sampling began on October 31 and ended on November  
PERMITIEE NAME/ADDRESS       (Include Facility Name/location if Different)                         NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)                                                           Form Approved .
: 5. This is the revised October 2010 DMR for 101T. Toxicity report is included. EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No 2040-0004 Address . ...!!_OX 2000 ___________ _
MAJOR DISCHARGE MONITORING REPORT                   (DMR)
____ _
Nam~_N~SEOU~A~UCLEARP~~ -- -                                                                                                                                                  (SUBR 01)                               OMB No 2040-0004 Address _p~ ....!!_OX 2000_ _ _ _ _ _ _ _ _ _ _ _
_________ _ ATIN: stephanie A. Howard PARAMETER X TEMPERATURE, WATER DEG. SAMPLE CENTIGRADE MEASUREMENT 00010 z 0 0 PERMIT INSTREAM MONITORING REQUIREMENT 1rEMPERATURE, WATER DEG. SAMPLE CENTIGRADE MEASUREMENT 00010 1 0 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT rEMP. DIFF. BETWEEN SAMP. & SAMPLE UPSTRM DEG.C MEASUREMENT 00016 1 w 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT PH SAMPLE MEASUREMENT 00400 1 0 0 PERMrr EFFLUENT GROSS VALUE REQUIREMENT SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 00530 1 0 0 PERMrr EFFLUENT GROSS VALUE REQUIREMENT OIL AND GREASE SAMPLE MEASUREMENT
- - - ~INT~~~~~A-SQ~ - --- ---
()0556 1 0 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 50050 1 0 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT E -J I DISCHARG:
- - - ~W~~AI~ffl~3~- - - - --- -
I lx* .. l MQ MQ 1 ... 1 From 10 11 01 To 10 11 30 DIFFUSER DISCHARGE EFFLUENT
Faclli~_NA~~~ffi~UCLE~~NT _ _ _ _ _
*** NO DISCHARGE D ... NOTE: Read instructions before completinll this form OUANTrrY OR LOADING OUALrrv OR CONCENTRATION NO. FREQUENCY EX OF AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS
E-=~: s~R JI                    5 DISCHARG:    ~~M~ER I F- FINAL DIFFUSER DISCHARGE r~rNGFt LoC~io~A~TONCNIT_              ___ ___ __ _                                                                                                                                  EFFLUENT ATIN: stephanie A. Howard                                                                        From lx*10.. l    MQ 11                01  To 10 MQ 11 1 ...
********  
30 1   *** NO DISCHARGE          D ...
******** ********  
NOTE: Read instructions before completinll this form X
********
PARAMETER                                                                OUANTrrY OR LOADING                                                      OUALrrv OR CONCENTRATION                            NO. FREQUENCY        SAMPLE EX        OF          TYPE AVERAGE                    MAXIMUM              UNITS                MINIMUM            AVERAGE            MAXIMUM            UNITS            ANALYSIS T EMPERATURE, WATER DEG.                        SAMPLE                     ********                  ********                                  ********          ********            20.7                        0      30 I 30      MODELD CENTIGRADE                                  MEASUREMENT                                                                         **                                                                        04 00010      z    0     0                         PERMIT
20.7 0 30 I 30 ** 04 ********  
                                                                          ********                   ********               ****               ********           ********             30.5            DEG.C.                SEE        CKREQ REQUIREMENT INSTREAM MONITORING                                                                                                                                                                  DAILYMX                                PERMIT 1rEMPERATURE, WATER DEG.                        SAMPLE MEASUREMENT
********  
                                                                            ********                 ********                  ..              ~*******           ********             32.9              04        0      30 I 30      RCORDR CENTIGRADE 00010      1    0 EFFLUENT GROSS VALUE 0                          PERMIT REQUIREMENT
**** ********  
                                                                                                                                                                    ********         REPORT DAILYMX DEG.C.               SEE PERMIT CKREQ rEMP. DIFF. BETWEEN SAMP. &                    SAMPLE MEASUREMENT
********
                                                                            ********                 ********                   ..             ********           ********             3.0              04        0      30 I 30      CALCTD UPSTRM DEG.C 00016      1    w    0                          PERMIT REQUIREMENT
30.5 DEG.C. SEE DAILYMX PERMIT ********
                                                                                                                              ....              ********          ********             5.0            DEG.C.             CO NTIN        CALCTD EFFLUENT GROSS VALUE PH                                              SAMPLE MEASUREMENT
********  
                                                                            ........                  ********                   ..                7.6            ********
********
DAILY MX 8.3               12        0 uous 6130          GRAB 00400      1    0     0                         PERMrr                    *******'*                 ********                                     6.0            ********             9.0              su            WEEKLY          GRAB REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                            MINIMUM                              MAXIMUM SOLIDS, TOTAL SUSPENDED                          SAMPLE MEASUREMENT
32.9 0 30 I 30 .. 04 ********  
                                                                            ********                 ********                    ..             ********               5                5                19        0      1 I 30        GRAB 00530      1    0    0                          PERMrr REQUIREMENT
********  
                                                                            ********                 ********                ....             ********               30                100              MG/L          MONTHLY          GRAB EFFLUENT GROSS VALUE                                                                                                                                              MOAVO              DAILYMX OIL AND GREASE                                    SAMPLE                    ********                 ********
-********  
                                                                                                                                                ********               <6                <6              19        0       1 I 30       GRAB MEASUREMENT
********
()0556    1    0    0                          PERMIT REQUIREMENT
REPORT DEG.C. SEE DAILYMX PERMIT ********
                                                                            ********                 ********                 ....             ********               15                20              MG/L           MONTHLY           GRAB EFFLUENT GROSS VALUE                                                                                                                                              MOAVC              DAILYMX FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT
********  
                                                                            ********                   1662                    03
********
                                                                                                                                                  ********          *******'*         ********            ..      0     30 I 30       RCORDR 50050      1    0    0                          PERMIT REQUIREMENT
********
                                                                            ********                REPORT                  MGD                ********           ********           ********
3.0 0 30 I 30 .. 04 ********  
                                                                                                                                                                                                            ....            CONTIN        RCORDR EFFLUENT GROSS VALUE                                                                              DAILYMX                                                                                                                  uous (t~~~CZ:,;oem NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penally of law lhal this document and all attachments were prepared under my                                                                    TELEPHONE                    DATE direction or supervision In accordance With a *Ystem destgned to assuru lha1 qualified personnel Michael D. Skaggs                    properly gather and evaluate lhe information submitted. Based on my inquiry of the person or persons who manage the syslem, or those persons directly responsible for galhering lhe 1nformation, the infonnation submitted is , to the best of my knowledge and belief, lrue, accurate,                                                423        843-7001        10      12    08 Sequoyah Site Vice President              ~< nd complete I am aware that there are significant penal lies for submilling false infonnation, Including the possibility or fine and imprisonment for knowing violations SIGNATURE OF PRINCIPAL EXECUTIVE                 I
********  
                                                                                                                                                                                                    ~
.... ********  
OFFICER OR AUTHORIZED AGENT                        NUMBER        YEAR      MO    DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (f?eference all attachments flere No closed mode operation . The following information is included in an attachment: 1. CCW data 2. veliger monitoring data 3. toxicity report EPA Fonn 3320-1 (REV 3/99)          Previous editions may be used                                                                                                                                                      Page 1 of 2
********
 
5.0 DEG.C. CO NT IN DAILY MX uous ........ ********
DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected        Hydrocarbons              Analysis Date/Time      Analyst Method No water would come out of the pump. No sample could be obtained.
7.6 ********
CCWCHANNEL Extractable Petroleum Date/Time Collected        Hydrocarbons              Analysis Date/Time      Analyst Method 11/09/2010@ 1030            <0.10 mg/1              11/12/2010@ 0403          KLM    EPH
8.3 0 6130 .. 12 *******'*  
 
********
Mean# of                                NOTES:%
,.. .........
Mean# of            Water                          Water                SUB Sample Date          %Settlers Temp. ("C) Sample Date  Asiatic            LOCATION  LOCATION Gravid Asiatic    COLLECTED BY ZM/m3                                            Temp. ("C)
6.0 ********
Clams/m3                                  Clam 11/03/2009    133      0        16      11/03/2009    76      16      In plant  RCW                          CMW 11/10/2009    417      6.1       16      11/10/2009    25      16      lnplant    RCW                          CMW 11/17/2009    269      0        16      11/17/2009      0       16      In plant  RCW                          CMW 11/24/2009    36      50        15      11/24/2009    18      15      lnplant    RCW                          CMW 12/01/2009    32      0        13.5    12/01/2009     0     13.5      In plant   RCW                           WE 12/08/2009     38      0        11     12/08/2009     0      11      In plant  RCW                         CMW 01/05/2010      0      0         6      01/05/2010      0       6      In plant   RCW                             8 01/12/2010      0      0          5      01/12/2010      0        5      In plant  RCW 01/19/2010      0      0         6      01/19/2010      0       6      In plant   RCW                             p 01/26/2010    32      0         7.5    01/26/2009     0       7.5      In plant   RCW                           NRT 02/02/2010     0       0         7      02/02/2010     0       7      In plant   RCW                       MSW/WDT 02/09/2010     0       0         8      02/09/2010     0       8      In plant   RCW                         BLITC 02/16/2010     0       0         5      02/16/2010     0       5      In plant   RCW                           BJ 02/23/2010   11.7      0         7     02/23/2010      0       7       In plant   RCW                           BJ 03/02/2010     0       0         6      03/02/2010     0       6      In plant   RCW                           PB 03/09/2010     0       0         8     03/09/2010     0       8       lnplant    RCW                           MJW 03/16/2010     0       0         10      03/16/2010     0       10      In plant   RCW                           BC 03/23/2010     14      0        11     03/23/2010     0       11      In plant   RCW                           BC 03/30/2010     0       0         14      03/30/2010     0       14      lnplant    RCW                         BAPO Apr-2010                                                                                                   no samples collected May-2010                                                                                                   no samples collected Jun-2010                                                                                                   no samples collected Jul-2010                                                                                                   no samples collected Aug-2010                                                                                                   no samples collected Sep-2010                                                                                                   no samples collected Oct-2010                                                                                                   no samples collected Nov-2010                                                                                                   no samples collected
9.0 su WEEKLY MINIMUM MAXIMUM ********  
 
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December 6, 2010 Bradley M. Love, OPS 5N-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, NOVEMBER, 2010 Per your request, I am only submitting an electronic copy of the subject report.
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The report provides results of compliance testing using fathead minnows and daphnids. Outfall101, samples collected October 31-November 5, showed no toxic effects to fathead minnows or daphnids.
5 5 0 1 I 30 .. 19 ********  
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30 100 MG/L MONTHLY MOAVO DAILYMX ********  
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15 20 MG/L MONTHLY MOAVC DAILYMX ********
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.... CONTIN DAILYMX uous -SAMPLE TYPE MODELD CKREQ RCORDR CKREQ CALCTD CALCTD GRAB GRAB GRAB GRAB GRAB GRAB RCORDR RCORDR NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penally of law lhal this document and all attachments were prepared under my TELEPHONE DATE direction or supervision In accordance With a *Ystem destgned to assuru lha1 qualified personnel Michael D. Skaggs properly gather and evaluate lhe information submitted
. Based on my inquiry of the person or persons who manage the syslem, or those persons directly responsible for galhering lhe 423 843-7001 10 12 08 1nformation, the infonnation submitted is , to the best of my knowledge and belief, lrue, accurate, Sequoyah Site Vice President complete I am aware that there are significant penal lies for submilling false infonnation, SIGNATURE OF PRINCIPAL EXECUTIVE I Including the possibility or fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED -COMMENTS AND EXPLANATION OF ANY VIOLATIONS (f?eference all attachments flere No closed mode operation
. The following information is included in an attachment:
: 1. CCW data 2. veliger monitoring data 3. toxicity report EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2 DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained.
CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 11/09/2010@
1030 <0.10 mg/1 11/12/2010@
0403 KLM EPH Mean# of Water Mean# of Water SUB NOTES:% Sample Date ZM/m3 %Settlers Temp. ("C) Sample Date Asiatic Temp. ("C) LOCATION LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 11/03/2009 133 0 16 11/03/2009 76 16 In plant RCW CMW 11/10/2009 417 6.1 16 11/10/2009 25 16 lnplant RCW CMW 11/17/2009 269 0 16 11/17/2009 0 16 In plant RCW CMW 11/24/2009 36 50 15 11/24/2009 18 15 lnplant RCW CMW 12/01/2009 32 0 13.5 12/01/2009 0 13.5 In plant RCW WE 12/08/2009 38 0 11 12/08/2009 0 11 In plant RCW CMW 01/05/2010 0 0 6 01/05/2010 0 6 In plant RCW 8 01/12/2010 0 0 5 01/12/2010 0 5 In plant RCW 01/19/2010 0 0 6 01/19/2010 0 6 In plant RCW p 01/26/2010 32 0 7.5 01/26/2009 0 7.5 In plant RCW NRT 02/02/2010 0 0 7 02/02/2010 0 7 In plant RCW MSW/WDT 02/09/2010 0 0 8 02/09/2010 0 8 In plant RCW BLITC 02/16/2010 0 0 5 02/16/2010 0 5 In plant RCW BJ 02/23/2010 11.7 0 7 02/23/2010 0 7 In plant RCW BJ 03/02/2010 0 0 6 03/02/2010 0 6 In plant RCW PB 03/09/2010 0 0 8 03/09/2010 0 8 lnplant RCW MJW 03/16/2010 0 0 10 03/16/2010 0 10 In plant RCW BC 03/23/2010 14 0 11 03/23/2010 0 11 In plant RCW BC 03/30/2010 0 0 14 03/30/2010 0 14 lnplant RCW BAPO Apr-2010 no samples collected May-2010 no samples collected Jun-2010 no samples collected Jul-2010 no samples collected Aug-2010 no samples collected Sep-2010 no samples collected Oct-2010 no samples collected Nov-2010 no samples collected December 6, 2010 Bradley M. Love, OPS 5N-SQN SEQUOY AH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, NOVEMBER, 2010 Per your request, I am only submitting an electronic copy of the subject report. The report provides results of compliance testing using fathead minnows and daphnids.
Outfall101, samples collected October 31-November 5, showed no toxic effects to fathead minnows or daphnids.
The resulting IC25 values for both species were > 100 percent.
The resulting IC25 values for both species were > 100 percent.
Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services-West CEB2L-M Attachment cc (Attachment):  
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
: Sherrard, R. M., PSC 1X-C Files, OE&R, MPB 1E-M SQN November 201 OM TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE SUMMARY Report Date: December  
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
: 6. 2010 1. Facility I Discharger:
Cynthia L. Russell Biologist Environmental Engineering Services- West CEB2L-M Attachment cc (Attachment):
Sequoyah Nuclear Plant I TV A 2. County I State: Hamilton I Tennessee  
Sherrard, R. M., PSC 1X-C Files, OE&R, MPB 1E-M SQN November 201 OM
: 3. NPDES Permit#:
 
TN0026450  
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE  
: 4. Type of Facility:
 
Nuclear-Fueled Electric Generating Plant 5. Design Flow (MGD): 1.579 6. Receiving Stream: Tennessee River (TRM 483.6) 7. 1Q10: 3.491 8. Outfall Tested: 101 9. Dates Sampled:
==SUMMARY==
October 31-November 5, 2010 10. Average Flow on Days Sampled (MGD): 916.91. 918.12. 916.45 11. Pertinent Site Conditions:
 
Towerbrom 960 (oxidizing biocide used for mollusk control) was injected into the RCW beginning 11/1110 @ 0900 through 1115110 @ 2359. During toxicity sampling three of the six condenser circulating water (CCW) pumps were in service and Unit 1 was shut down for a refueling outage. 12. Test Dates: November 2-9. 2010 13. Test Type: Short-term Chronic Definitive  
Report Date: December 6. 2010
: 14. Test Species:
: 1. Facility I Discharger: Sequoyah Nuclear Plant I TVA
Fathead Minnows (Pimephalespromelas)
: 2. County I State: Hamilton I Tennessee
Daphnids  
: 3. NPDES Permit#: TN0026450
( Ceriodaphnia dubia) 15. Concentrations Tested(%):
: 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
Outfall101:
: 5. Design Flow (MGD): 1.579
11.3. 22.6. 45.2. 72.6. 100 Intake: 100.0 Pimephales promelas:
: 6. Receiving Stream: Tennessee River (TRM 483.6)
UV treated Outfall101:
: 7. 1Q10: 3.491
11.3. 22.6. 45.2. 72.6, 100 UV treated Intake: 100.0 16. Permit Limit Endpoint(%):
: 8. Outfall Tested: 101
Outfall101:
: 9. Dates Sampled: October 31-November 5, 2010
IC2s = 45.2% 17. Test Results:
: 10. Average Flow on Days Sampled (MGD): 916.91. 918.12. 916.45
Outfall101:
: 11. Pertinent Site Conditions: Towerbrom 960 (oxidizing biocide used for mollusk control) was injected into the RCW beginning 11/1110 @ 0900 through 1115110 @ 2359. During toxicity sampling three of the six condenser circulating water (CCW) pumps were in service and Unit 1 was shut down for a refueling outage.
Pimephales promelas:
: 12. Test Dates: November 2-9. 2010
IC2s > 100% Page 1 of105 Ceriodaphnia dubia: Kzs > 100% UV treated Outfall101:
: 13. Test Type: Short-term Chronic Definitive
Pimephales promelas:
: 14. Test Species: Fathead Minnows (Pimephalespromelas)
ICzs > 100% 18. Facility Contact:
Daphnids (Ceriodaphnia dubia)
Stephanie A. Howard Phone#: (423) 843-6700  
: 15. Concentrations Tested(%): Outfall101: 11.3. 22.6. 45.2. 72.6. 100 Intake: 100.0 Pimephales promelas: UV treated Outfall101: 11.3. 22.6. 45.2. 72.6, 100 UV treated Intake: 100.0
: 19. Consulting I Testing Lab: Environmental Testing Solutions.
: 16. Permit Limit Endpoint(%): Outfall101: IC2s = 45.2%
Inc. 20. Lab Contact:
: 17. Test Results: Outfall101: Pimephales promelas: IC2s > 100%
Jim Sumner Phone #: (828) 350-9364 Phone #: (256) 386-2755  
Page 1 of105
: 21. TV A Contact:
 
Cynthia L. Russell 22. Notes: Outfall101 samples collected October 31-November 5, 2010, showed no toxic effects to fathead minnows or daphnids.
Ceriodaphnia dubia: Kzs > 100%
Page2of105 The resulting ICzs values, for both species, were> 100 percent.
UV treated Outfall101: Pimephales promelas: ICzs > 100%
Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
: 18. Facility
 
==Contact:==
Stephanie A. Howard                   Phone#: (423) 843-6700
: 19. Consulting I Testing Lab: Environmental Testing Solutions. Inc.
: 20. Lab
 
==Contact:==
Jim Sumner                                 Phone #: (828) 350-9364
: 21. TVA
 
==Contact:==
Cynthia L. Russell                        Phone #: (256) 386-2755
: 22. Notes: Outfall101 samples collected October 31-November 5, 2010, showed no toxic effects to fathead minnows or daphnids.
The resulting ICzs values, for both species, were> 100 percent.
Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
METHODS SUMMARY Samples:  
Page2of105
: 1. Sampling Point: Outfall 101. Intake 2. Sample Type: Composite  
 
METHODS  
 
==SUMMARY==
 
Samples:
: 1. Sampling Point: Outfall 101. Intake
: 2. Sample Type: Composite
: 3. Sample Information:
: 3. Sample Information:
Date Date Date (MM-DD-YY)  
Date                     Date                                           Date (MM-DD-YY)               (MM-DD-YY)           Arrival     Initial     (MM-DD-YY)
(MM-DD-YY) Arrival Initial (MM-DD-YY)
Sample           Time (ET)               Time (ET)           Temp. t     TRC*           Time (ET)
Sample Time (ET) Time (ET) Temp. t TRC* Time (ET) ID Collected Received (OC) (mg/L) Last Used By 101 10-31-10 0743 to 11-01-10 1456 0.8, 1.0 <0.10 11-02-10 1225 11-01-10 0643 11-03-10 1125 Intake 10-31-10 0836 to 11-01-10 1456 0.7 <0.10 11-02-10 1225 11-01-10 0736 11-03-10 1125 101 11-02-10 0727 to 11-03-10 1430 0.8, 0.8 <0.10 11-04-10 1240 11-03-10 0627 11-05-10 1125 Intake 11-02-10 0738 to 11-03-10 1430 1.0 <0.10 11-04-10 1240 11-03-10 0638 11-05-10 1125 11-04-10 0741 to 11-06-10 1130 101 11-05-10 0641 11-05-10 1500 1.2, 1.2 <0.10 11-07-10 1128 11-08-10 1135 11-04-10 0758 to 11-06-10 1130 Intake 11-05-10 1500 1.3 <0.10 11-07-10 1128 11-05-10 0658 11-08-10 1135 *TRC = Total Residual Chlonne tsamples were collected in two 2.5 gallon cubitainers.
ID             Collected               Received             (OC)     (mg/L)       Last Used By 10-31-10 0743 to                                                         11-02-10 1225 101                                  11-01-10 1456         0.8, 1.0     <0.10 11-01-10 0643                                                           11-03-10 1125 10-31-10 0836 to                                                         11-02-10 1225 Intake                                11-01-10 1456           0.7       <0.10 11-01-10 0736                                                           11-03-10 1125 11-02-10 0727 to                                                         11-04-10 1240 101                                  11-03-10 1430         0.8, 0.8     <0.10 11-03-10 0627                                                           11-05-10 1125 11-02-10 0738 to                                                         11-04-10 1240 Intake                                11-03-10 1430           1.0       <0.10 11-03-10 0638                                                           11-05-10 1125 11-06-10 1130 11-04-10 0741 to 101                                  11-05-10 1500         1.2, 1.2     <0.10       11-07-10 1128 11-05-10 0641 11-08-10 1135 11-06-10 1130 11-04-10 0758 to Intake                                 11-05-10 1500           1.3       <0.10       11-07-10 1128 11-05-10 0658 11-08-10 1135
Temperature was measured in each cubitainer upon arrival.  
          *TRC = Total Residual Chlonne tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
: 4. Sample Manipulation:
: 4. Sample Manipulation: Samples from Outfall101 and intake were warmed to test temperature (25.0 + 1.0&deg;C) in a warm water bath.
Samples from Outfall101 and intake were warmed to test temperature (25.0 + 1.0&deg;C) in a warm water bath. Page3of105 Aliquots of0utfall101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics.
Aliquots of0utfall101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics.
Inc.) for 2 minutes.
Inc.) for 2 minutes.
Pirnephales prornelas Test Organisms:  
Page3of105
: 1. Source: Aquatox, Inc. 2. Age: 19.57-19.75 hours old Test Method Summary:  
 
: 1. Test Conditions:
Pirnephales prornelas                       Ceriodaphnia dubia Test Organisms:
Static, Renewal 2. Test Duration:  
: 1. Source:                     Aquatox, Inc.                         In-house Cultures
: 3. Control I Dilution Water: Moderately Hard Synthetic  
: 2. Age:                         19.57- 19.75 hours old              < 24-hours old Test Method Summary:
: 4. Number of Replicates:  
: 1. Test Conditions:           Static, Renewal                       Static. Renewal
.4 5. Organisms per Replicate:
: 2. Test Duration:                                                   Until at least 60% of control females have 3 broods
10 6. Test Initiation:  
: 3. Control I Dilution Water: Moderately Hard Synthetic              Moderately Hard Synthetic
(Date/Time)
: 4. Number of Replicates:       .4                                   10
Outfall101 11-02-10 1145 ET UV Treated Outfall 101 11-02-10 1134 ET 7. Test Termination:  
: 5. Organisms per Replicate: 10                                      1
(Date/Time)
: 6. Test Initiation: (Date/Time)
Outfall101 11-09-10 1055 ET UV Treated Outfall 101 11-09-10 1040 ET 8. Test Temperature:
Outfall101         11-02-10 1145 ET              11-02-10 1225 ET UV Treated Outfall 101         11-02-10 1134 ET
Outfall101:
: 7. Test Termination: (Date/Time)
Mean= 24.7&deg;C (24.4 -25.3&deg;C) Ceriodaphnia dubia In-house Cultures
Outfall101         11-09-10 1055 ET              11-09-10 1143 ET UV Treated Outfall 101         11-09-10 1040 ET
< 24-hours old Static. Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic 10 1 11-02-10 1225 ET 11-09-10 1143 ET Mean= 24.9&deg;C (24. 7 -25.2&deg;C) Test Temperature:
: 8. Test Temperature: Outfall101:       Mean= 24.7&deg;C                 Mean= 24.9&deg;C (24.4 - 25.3&deg;C)              (24. 7 - 25.2&deg;C)
UV-Treated Outfall101:
Test Temperature: UV-Treated Outfall101:         Mean = 24.8&deg;C (24.4 - 25.2&deg;C)
Mean = 24.8&deg;C (24.4 -25.2&deg;C) 9. Physical I Chemical Measurements:
: 9. Physical I Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
Alkalinity,  
: 10. Statistics:       Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyyille, CA).
: hardness, total residual  
Page4 of lOS
: chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration.
 
Pre-and exposure test solutions were analyzed daily for pH and dissolved oxygen. 10. Statistics:
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific  
: 1. Results of a   Pimephales pro me/as Chronic/ 7-day Toxicity Test.
: Software, McKinneyyille, CA). Page4 of lOS TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1. Results of a Pimephales pro me/as Chronic/
(Genus species)             (Type I Duration)
7 -day Toxicity Test. (Genus species)  
(Type I Duration)
Conducted November 2-9. 2010 using effluent from Outfall101.
Conducted November 2-9. 2010 using effluent from Outfall101.
Test Percent Surviving Solutions (time interval used -days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 98 98 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 95 95 Test Solutions Mean Dry Weight (mg) (% Effluent)  
Test                             Percent Surviving Solutions                       (time interval used - days)
(replicate number) 1 2 3 4 Mean Control 0.767 0.766 0.956 0.837 0.832 11.3% 0.793 0.702 0.773 0.721 0.747 22.6% 0.698 0.737 0.800 0.803 0.760 45.2% 0.739 0.729 0.752 0.706 0.732 72.6% 0.725 0.802 0.773 0.790 0.773 100.0% 0.827 0.913 0.709 0.782 0.808 Intake 0.736 0.773 0.814 0.629 0.738 ICzs Value: > 100% Calculated TU Estimates:  
(% Effluent)     1       2       3       4         5       6         7 Control       100     100   100       100       100     100     100 11.3%       100     100   100       100       100     100     100 22.6%         100     100   100       100       100     100     100 45.2%         100     100   100       100       100     98       98 72.6%         100     100   100       100       100     100     100 100.0%       100     100   100       100       100     100     100 Intake       100     100   100       100       100     95       95 Mean Dry Weight (mg)
< 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso:
Test Solutions (replicate number)
TUc = 100/ ICzs Page 5 of lOS TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/
(% Effluent) 1           2           3           4       Mean Control         0.767       0.766         0.956       0.837     0.832 11.3%         0.793       0.702         0.773       0.721     0.747 22.6%           0.698       0.737         0.800       0.803     0.760 45.2%           0.739       0.729         0.752       0.706     0.732 72.6%           0.725       0.802         0.773       0.790     0.773 100.0%         0.827       0.913         0.709       0.782     0.808 Intake         0.736       0.773         0.814       0.629     0.738 ICzs Value: > 100%                   Calculated TU Estimates: < 1.0 TUc*
7 -day Toxicity Test. (Genus species)  
Permit Limit: 45.2%
(Type I Duration)
Permit Limit: 2.2 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
                *TUa = 100/LCso: TUc = 100/ ICzs Page 5 of lOS
 
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
: 2. Results of a   Ceriodaphnia dubia   Chronic/ 7-day Toxicity Test.
(Genus species)             (Type I Duration)
Conducted November 2-9. 2010 using effluent from Outfall101.
Conducted November 2-9. 2010 using effluent from Outfall101.
Percent Surviving Test (time interval used -days) Solutions 1 2 3 4 5 6 7 (% Effluent)
Percent Surviving Test                       (time interval used - days)
Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction  
Solutions 1       2       3       4         5     6       7
(#young/female/7 days) (% Effluent)
(% Effluent)
Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 32 29 30 28 32 28 31 28 30 29.8 11.3% 32 32 30 32 28 32 30 33 29 30 30.8 22.6% 35 29 32 35 31 34 31 29 30 33 31.9 45.2% 36 34 31 34 36 36 32 30 34 33 33.6 72.6% 34 31 31 38 36 35 32 34 36 37 34.4 100.0% 39 35 36 36 34 37 34 38 33 36 35.8 ICzs Value: > 100% Calculated TU Estimates:  
Control       100     100     100     100       100   100     100 11.3%       100     100     100       100       100   100     100 22.6%         100     100     100       100       100   100     100 45.2%         100     100     100       100       100   100     100 72.6%         100     100     100       100       100   100     100 100.0%       100     100     100       100       100   100     100 Reproduction (#young/female/7 days)
< 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso:
Test Solutions Data (replicate number)
TUc = 100/ ICzs Page 6 of lOS TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/
(% Effluent) 1   2     3   4     5     6     7     8   9 10   Mean Control     30   32   29   30   28   32   28   31 28   30 29.8 11.3%       32   32   30   32   28   32   30   33 29   30 30.8 22.6%       35   29   32   35   31   34   31   29 30   33 31.9 45.2%       36   34   31   34   36   36   32   30 34   33 33.6 72.6%       34   31   31   38   36   35   32   34 36   37 34.4 100.0%       39   35   36   36   34   37   34   38 33   36 35.8 ICzs Value: > 100%               Calculated TU Estimates: < 1.0 TUc*
7 -day Toxicity Test. (Genus species)  
Permit Limit: 45.2%
(Type I Duration)
Permit Limit: 2.2 TUc 95% Confidence Limits:
Conducted November 2-9. 2010 using water from Intake Percent Surviving Test (time interval used -days) Solutions 1 2 3 4 5 6 7 (% Effluent)
Upper Limit: NA Lower Limit: NA
Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction  
                *TUa = 100/LCso: TUc = 100/ ICzs Page 6 of lOS
(#young/female/7 days) (% Effluent)
 
Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 31 31 28 28 30 33 29 31 31 30.1 Intake 32 33 28 35 34 35 34 31 32 33 32.7 IC2s Value: > 100% Calculated TU Estimates:  
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
< 1.0 TUc* Permit Limit: N/ A Permit Limit: N/ A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso:
: 2. Results of a   Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
TUc = 100IIC2s Page 7 of105 TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets) 3. Results of a Pimephales promelas Chronic/
(Genus species)             (Type I Duration)
7 -day Toxicity Test. (Genus species)  
Conducted November 2-9. 2010 using water from Intake Percent Surviving Test                         (time interval used - days)
(Type I Duration)
Solutions 1       2       3       4         5     6       7
(% Effluent)
Control       100     100     100     100       100   100     100 Intake       100     100     100     100       100   100     100 Reproduction (#young/female/7 days)
Test Solutions Data (replicate number)
(% Effluent) 1   2     3   4     5     6     7     8   9 10   Mean Control       29   31   31   28   28   30   33   29 31 31   30.1 Intake       32   33   28   35   34   35   34   31 32 33   32.7 IC2s Value: > 100%               Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
              *TUa = 100/LCso: TUc = 100IIC2s Page 7 of105
 
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
: 3. Results of a   Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)               (Type I Duration)
Conducted November 2-9. 2010 using effluent from UV Treated Outfall101.
Conducted November 2-9. 2010 using effluent from UV Treated Outfall101.
Test Percent Surviving Solutions (time interval used-days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg) (% Effluent) reR_licate number) 1 2 3 4 Mean Control 0.857 0.760 0.821 0.910 0.837 11.3% 0.879 0.908. 0.826 0.830 0.861 22.6% 0.828 0.868 0.951 0.731 0.845 45.2% 0.837 0.901 0.897 0.834 0.867 72.6% 0.820 0.829 0.854 0.952 0.864 100.0% 0.891 0.800 0.929 0.907 0.882 Intake 0.879 0.876 0.875 0.935 0.891 ICzs Value: > 100% Calculated TU Estimates:  
Test                             Percent Surviving Solutions                     (time interval used- days)
< 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 100/LCso:
(% Effluent)       1       2         3         4       5       6       7 Control       100     100       100     100     100     100     100 11.3%       100     100       100     100     100     100     100 22.6%         100     100       100     100     100     100     100 45.2%         100     100     100       100     100     100     100 72.6%         100     100     100       100     100     100     100 100.0%       100     100     100       100     100     100     100 Intake       100     100     100       100     100     100     100 Mean Dry Weight (mg)
TUc = 100/ ICzs REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) Species Date Time Duration Toxicant Results (ICzs) Pimephales promelas November 2-9, 2010 1155 7-days KCl 0.70 g/L Ceriodaphnia dubia November 2-9, 2010 1208 6-days NaCl 1.09 g/L Page8of105 PHYSICAUCHEMICAL SUMMARY Water Chemistry Mean Values and Ranges for Pimephales promelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfull 101 perfonned November 02-09, 2010. Test Sample ID Temperature (0C} Initial Control 24.8 24.7 -24.9 11.3% 24.8 .s 24.7 -25.0 24.9 22.6% 24.7 -25.0 l: !:)., 24.9 tl 45.2% 24.7 -25.0 -a 't 72.6% 24.9 .&sect; 24.7 -25.1 Q, 24.9 100.0% 24.7 -25.3 Intake 24.9 24.8 -25.2 Control 24.8 24.7 -24.9 11.3% 24.8 24.7 -25.0 .s 24.8 '"'l 22.6% 24.7 -25.0 .s 24.9 == 45.2% ..::: 24.7 -25.0 &sect;-'1:1 24.9 72.6% t:l 24.8 -25.0 100.0% 25.0 24.8 -25.2 Intake 24.9 24.7 -25.1 Overall temperature
Test Solutions reR_licate number)
("C) Pimephales promelas Ceriodaphnia dubia Page 9ofl05 Final 24.6 24.5 -24.7 24.6 24.4 -24.9 24.7 24.6 -24.8 24.7 24.5 -24.8 24.6 24.5 -24.8 24.6 24.5 -24.8 24.6 24.4 -24.8 24.9 24.7 -25.2 24.9 24.8 -25.2 25.0 24.8 -25.2 25.0 24.8 -25.1 25.0 24.7 -25.2 24.9 24.7 -25.2 25.0 24.9 -25.2 Average 24.7 24.9 Dissolved Oxygen (mg/L) Initial Final 7.6 7.2 7.5 -7.7 6.6 -7.6 7.7 7.2 7.5 -7.9 6.6 -7.6 7.7 7.1 7.5 -8.0 6.4 -7.7 7.8 7.1 7.5 -8.1 6.4 -7.6 7.8 7.1 7.5 -8.0 6.5 -7.7 7.8 7.1 7.5 -8.1 6.5 -7.7 7.9 7.2 7.6 -8.2 6.7 -7.8 7.6 7.7 7.5 -7.7 7.4 -8.0 7.7 7.8 7.5 -7.9 7.5 -8.0 7.7 7.8 7.5 -8.0 7.5 -8.0 7.8 7.7 7.5 -8.1 7.5 -8.0 7.8 7.7 7.5 -8.0 7.4 -8.0 7.8 7.7 7.5 -8.1 7.5 -8.0 7.9 7.8 7.6 -8.2 7.5 -8.0 Minimum Maximum 24.4 25.3 24.7 25.2 pH(S.U.)
(% Effluent) 1         2             3           4       Mean Control       0.857     0.760       0.821       0.910       0.837 11.3%       0.879     0.908.       0.826       0.830       0.861 22.6%       0.828     0.868       0.951       0.731       0.845 45.2%       0.837     0.901       0.897       0.834       0.867 72.6%       0.820     0.829       0.854       0.952       0.864 100.0%       0.891     0.800       0.929       0.907       0.882 Intake       0.879     0.876       0.875       0.935       0.891 ICzs Value: > 100%                     Calculated TU Estimates: < 1.0 TUc*
Conductance Alkalinity Hardness Total Residual Initial Final (!lmhos/cm)
95% Confidence Limits:
(mg!L CaC03) (mg!L CaC03) Chlorine (mg!L) 7.75 7.64 315 63 91 7.67 -7.79 7.43
Upper Limit: NA Lower Limit: NA
-7.82 305 -333 62 -64 89 -93 7.84 7.62 300 7.79 -7.92 7.39
              *TUa = 100/LCso: TUc = 100/ ICzs REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
-7.82 290 -310 7.83 7.60 286 7.80 -7.92 7.37 -7.80 280 -295 7.82 7.60 257 7.80 -7.90 7.37 -7.78 250 -266 7.80 7.59 220 7.76 -7.89 7.35 -7.78 204 -229 7.79 7.59 186 69 71 <0.10 7.72 -7.89 7.35 -7.78 179 -192 67 -71 70 -72 < 0.10 -< 0.10 7.76 7.59 187 67 69 < 0.10 7.68 -7.89 7.36 -7.76 182 -191 67 -67 68 -70 < 0.10 -< 0.10 7.75 7.82 315 63 91 7.67 -7.79 7.74 -7.90 305 -333 62 -64 89 -93 7.84 7.83 300 7.79 -7.92 7.71 -7.90 290 -310 7.83 7.82 286 7.80 -7.92 7.69 -7.90 280 -295 7.82 7.81 257 7.80 -7.90 7.68 -7.88 250 -266 7.80 7.81 220 7.76 -7.89 7.68 -7.87 204 -229 7.79 7.80 186 69 71 < 0.10 7.72 -7.89 7.67
Species                           Date               Time Duration Toxicant       Results (ICzs)
-7.86 179 -192 67 -71 70 -72 < 0.10 -< 0.10 7.76 7.81 187 67 69 < 0.10 7.68 -7.89 7.70 -7.88 182 -191 67 -67 68 -70 < 0.10 -< 0.10 PHYSICAUCHEMICAL SUMMARY Water Chemistry Mean Values and Ranges fur Pimephales promelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfull 101 perfunred November 02-09. 2010. Test Sample ID Temperature
Pimephales promelas         November 2-9, 2010         1155     7-days     KCl       0.70 g/L Ceriodaphnia dubia         November 2-9, 2010         1208     6-days     NaCl       1.09 g/L Page8of105
('C) Initial Control 24.9 24.7 -24.9 24.9 11.3% .., 24.9 -25.0 .s 25.0 22.6% IS 24.9 -25.0 25.0 Jl 45.2% ':1 24.9 -25.0 ..;:: 25.0 72.6% .&sect; 24.9 -25.1 =... 25.0 100.0% 24.9 -25.2 Intake 25.0 24.9 -25.1 Overall temperature
 
('C) Pimephales promelas Page 10 of 105 Final 24.7 24.6 -24.8 24.7 24.5 -24.8 24.7 24.5 -24.8 24.6 24.4 -24.8 24.6 24.5 -24.7 24.6 24.4 -24.9 24.6 24.4 -24.8 Average 24.8 Dissolved Oxygen (mg!L) Initial Final 7.8 7.2 7.5 -8.0 6.6 -7.7 7.8 7.2 7.5 -8.0 6.6 -7.8 7.8 7.2 7.5 -8.1 6.6 -7.7 7.8 7.1 7.5 -8.1 6.5 -7.7 7.8 7.1 7.5 -8.1 6.6 -7.7 7.9 7.1 7.5 -8.1 6.5 -7.7 8.0 7.2 7.6 -8.3 6.6 -8.0 Minimum Maximum 24.4 25.2 pH(S.U.)
PHYSICAUCHEMICAL  
Conductance Alkalinity Hardness Initial Final (ltmhos/cm)
 
(mg!L CaC03) (mg!L CaC03) 7.82 7.62 313 60 84 7.77 -7.88 7.28 -7.82 301 -329 60 -61 82 -86 7.82 7.61 296 7.78 -7.88 7.31 -7.80 290 -303 7.82 7.61 282 7.78 -7.88 7.32 -7.78 277 -290 7.81 7.59 256 7.77 -7.87 7.31
==SUMMARY==
-7.78 250 -260 7.80 7.60 223 7.76 -7.86 7.32
 
-7.78 217 -228 7.79 7.59 189 68 72 7.73 -7.87 7.29 -7.78 184 -196 67 -69 70 -74 7.76 7.58 186 68 73 7.68 -7.84 7.30 -7.80 178 -193 64 -71 70 -78 Total Residual Chlorine (mg!L) < 0.10 < 0.10 -< 0.10 < 0.10 < 0.10 -< 0.10 SUMMARY I CONCLUSIONS Page 11 of I 05 Outfall101 samples collected October 31-November 5, 2010, showed no toxic effects to fathead minnows or daphnids.
Water Chemistry Mean Values and Ranges for Pimephales promelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfull 101 perfonned November 02-09, 2010.
Test       Sample ID                       0 Temperature ( C}       Dissolved Oxygen (mg/L)            pH(S.U.)        Conductance    Alkalinity      Hardness      Total Residual Initial        Final        Initial      Final        Initial        Final  (!lmhos/cm) (mg!L CaC0 3) (mg!L CaC03 )        Chlorine (mg!L) 24.8         24.6            7.6        7.2          7.75          7.64        315            63              91 Control 24.7 - 24.9  24.5 - 24.7    7.5 -    7.7 6.6 -   7.6 7.67 - 7.79  7.43 - 7.82  305 - 333    62  -  64    89    - 93 24.8          24.6            7.7        7.2          7.84          7.62        300 11.3%
24.7 - 25.0   24.4 - 24.9    7.5 -    7.9 6.6 -    7.6 7.79 - 7.92  7.39 - 7.82  290 - 310
              .s                          24.9         24.7            7.7        7.1          7.83          7.60        286
                ~          22.6%
24.7 - 25.0   24.6 - 24.8   7.5 -    8.0 6.4 -    7.7 7.80 - 7.92  7.37 - 7.80  280 - 295 l:!:).,
24.9         24.7           7.8        7.1          7.82          7.60        257 tl        45.2%
              -a                      24.7 - 25.24.5 - 24.8   7.5 -   8.1 6.4 -   7.6 7.80 - 7.90  7.37 - 7.78  250 - 266
              't          72.6%
24.9          24.6            7.8         7.1          7.80          7.59        220
              .&sect; Q,
24.7 - 25.24.5 - 24.8    7.5 -   8.0 6.5 -   7.7 7.76 - 7.89  7.35 - 7.78  204 - 229 24.9         24.6           7.8        7.1          7.79          7.59        186            69              71              <0.10 100.0%
24.7 - 25.3  24.5 - 24.8   7.5 -   8.1 6.5 -   7.7 7.72 - 7.89  7.35 - 7.78  179 - 192    67  -  71    70    - 72    < 0.10 - < 0.10 24.9          24.6            7.9        7.2          7.76          7.59        187            67              69              < 0.10 Intake 24.8 - 25.2  24.4 - 24.8    7.6 -   8.2 6.7 -   7.8 7.68 - 7.89  7.36 - 7.76  182 - 191    67  -  67    68    - 70    < 0.10 - < 0.10 24.8         24.9           7.6        7.7         7.75          7.82        315            63              91 Control 24.7 - 24.9  24.7 - 25.2    7.5 -   7.7 7.4 -   8.0 7.67 - 7.79  7.74 - 7.90  305 - 333    62  -  64    89    - 93 24.8         24.9            7.7        7.8         7.84          7.83        300 11.3%
24.7 - 25.24.8 - 25.2   7.5 -    7.9 7.5 -  8.7.79 - 7.92  7.71 - 7.90  290 - 310
              .s
              '"'l                        24.8          25.0            7.7        7.8          7.83          7.82        286 22.6%
              ~                      24.7 - 25.0  24.8 - 25.2    7.5 -    8.0 7.5 -  8.0  7.80 - 7.92   7.69 - 7.90  280 - 295
              .s                          24.9          25.0            7.8        7.7         7.82          7.81        257
              ..:::==      45.2%
                &sect;-                   24.7 - 25.0  24.8 - 25.1    7.5 -    8.1 7.5 -  8.0 7.80 - 7.90  7.68 - 7.88  250 - 266
              '1:1                        24.9          25.0           7.8        7.7         7.80          7.81        220
              *~          72.6%
24.8 - 25.0   24.7 - 25.2    7.5 -    8.0 7.4 -   8.7.76 - 7.89   7.68 - 7.87  204 - 229 t:l 25.0          24.9            7.8        7.7         7.79          7.80        186            69               71              < 0.10 100.0%
24.8 - 25.2  24.7 - 25.2    7.5 -   8.1 7.5 -   8.7.72 - 7.89   7.67 - 7.86  179 - 192    67  -   71    70    - 72    < 0.10 - < 0.10 24.9          25.0            7.9        7.8          7.76          7.81        187            67               69              < 0.10 Intake 24.7 - 25.1  24.9 - 25.2    7.6 -    8.2 7.5 -  8.0 7.68 - 7.89   7.70 - 7.88 182 - 191     67   -   67     68   - 70     < 0.10 - < 0.10 Overall temperature ("C)                    Average      Minimum      Maximum Pimephales promelas           24.7          24.4        25.3 Ceriodaphnia dubia            24.9           24.7       25.2 Page 9ofl05
 
PHYSICAUCHEMICAL
 
==SUMMARY==
 
Water Chemistry Mean Values and Ranges fur Pimephales promelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfull 101 perfunred November 02-09. 2010.
Test      Sample ID      Temperature  ('C)      Dissolved Oxygen (mg!L)            pH(S.U.)          Conductance    Alkalinity      Hardness  Total Residual Initial      Final        Initial      Final        Initial        Final    (ltmhos/cm) (mg!L CaC03 ) (mg!L CaC03 )    Chlorine (mg!L) 24.9        24.7          7.8          7.2        7.82          7.62        313            60              84 Control 24.7 - 24.9 24.6 - 24.8  7.5 -    8.0 6.6 -   7.7 7.77 - 7.88  7.28 - 7.82  301 - 329    60    -  61    82    - 86 24.9        24.7          7.8           7.2        7.82          7.61        296 11.3%
                  ..,                24.9 - 25.0  24.5 - 24.7.5 -   8.0  6.6 -   7.8 7.78 - 7.88  7.31 - 7.80  290 - 303
                .s~
25.0         24.7           7.8           7.2         7.82          7.61        282 IS        22.6%
24.9 - 25.0 24.5 - 24.8   7.5 -    8.6.6 -   7.7 7.78 - 7.88  7.32 - 7.78  277 - 290
                  ~
                  ~                      25.0        24.6          7.8         7.1         7.81          7.59        256 Jl':1       45.2%
24.9 - 25.0  24.4 - 24.7.5 -   8.1 6.5 -   7.7 7.77 - 7.87  7.31 - 7.78  250 - 260
                  ~                      25.0         24.6          7.8          7.1        7.80          7.60        223 72.6%
                .&sect;                    24.9 - 25.1  24.5 - 24.7   7.5 -   8.1  6.6 -   7.7 7.76 - 7.86  7.32 - 7.78  217 - 228
                =...
25.0        24.6          7.9          7.1          7.79          7.59         189            68              72          < 0.10 100.0%
24.9 - 25.2  24.4 - 24.7.5 -    8.1  6.5 -   7.7 7.73 - 7.87   7.29 - 7.78   184 - 196     67   - 69     70   - 74 < 0.10 - < 0.10 25.0        24.6          8.0          7.2        7.76          7.58         186             68             73         < 0.10 Intake 24.9 - 25.1  24.4 - 24.8  7.6 -    8.3  6.6 -    8.0 7.68 - 7.84   7.30 - 7.80   178 - 193     64   - 71     70   - 78 < 0.10 -  < 0.10 Overall temperature ('C)                Average      Minimum      Maximum Pimephales promelas          24.8          24.4          25.2 Page 10 of 105
 
==SUMMARY==
I CONCLUSIONS Outfall101 samples collected October 31-November 5, 2010, showed no toxic effects to fathead minnows or daphnids.
The resulting ICzs values, for both species, were> 100 percent.
The resulting ICzs values, for both species, were> 100 percent.
Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
Appendix A ADDITIONAL TOXICITY TEST INFORMATION SUMMARY OF METHODS 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment.
Page 11 of I 05
Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.  
 
: 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment.
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
 
DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL  
==SUMMARY==
: 1. Pimephales promelas None 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS  
OF METHODS
: 1. Pimephales promelas None 2. Ceriodaphnia dubia None Page 12 of 105 PHYSICAL AND CHEMICAL METHODS 1. Reagents,  
: 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
: Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).  
: 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
: 2. Instruments:
DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL
All identification,  
: 1. Pimephales promelas None
: service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.  
: 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
: 3. Temperature was measured by SM 2550 B. 4. Dissolved oxygen was measured by SM 4500 0 G. 5. The pH was measured by SM 4500 H+ B. 6. Conductance was measured by SM 2510 B. 7. Alkalinity was measured by SM 2320 B. 8. Total hardness was measured by SM 2340 C. 9. Total residual chlorine was measured by ORION Electrode Method 97-70. QUALITY ASSURANCE Toxicity Test Methods:
: 1. Pimephales promelas None
All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013.
: 2. Ceriodaphnia dubia None Page 12 of 105
Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)  
 
: 1. Test Type: 7 -day chronic tests with results expressed as ICzs values in giL KCl or NaCl. 2. Standard Toxicant:
PHYSICAL AND CHEMICAL METHODS
Potassium Chloride (KCl crystalline) for Pimephales promelas.
: 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia. 3. Dilution Water Used: Moderately hard synthetic water. 4. Statistics:
: 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
ToxCalc software Version 5.0 was used for statistical analyses.
: 3. Temperature was measured by SM 2550 B.
Page 13 of 105 REFERENCES  
: 4. Dissolved oxygen was measured by SM 4500 0 G.
: 1. NPDES Permit No. TN0026450.  
: 5. The pH was measured by SM 4500 H+ B.
: 2. USEP A. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002). 3. Standard Methods for the Examination of Water and Wastewater.
: 6. Conductance was measured by SM 2510 B.
21st Edition, 2005. 4. Quality Assurance Program:
: 7. Alkalinity was measured by SM 2320 B.
Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
: 8. Total hardness was measured by SM 2340 C.
Page 14 ofl05 Page 15 of105 Sequoyah Nuclear Plant Biomonitoring November 2-9, 2010 Appendix B Diffuser Discharge Concentrations of Total Residual  
: 9. Total residual chlorine was measured by ORION Electrode Method 97-70.
: Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall101)
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
: Mollusks, During Toxicity Test Sampling, Date 08/18/1999 08/19/1999 08/20/1999 08/21/1999 08/22/1999 08/23/1999 08/24/1999
: 1. Test Type: 7-day chronic tests with results expressed as ICzs values in giL KCl or NaCl.
' Page 16 of 105 March 12, 1998-November 5, 2010 PCL-401 mg!L Copolymer 0.005 0.011 0.021 0.019 O.DlS O.DlS O.DlS 0.024 0.024 0.024 0.024 0.024 0.024 0.023 Table B-1. Sequoyah Nuclear Plant Diffuser (OutfalllOl)
: 2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas.
Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
: Mollusks, During Toxicity Test Sampling, Date 11/26/2001 11/27/2001
: 3. Dilution Water Used: Moderately hard synthetic water.
. 11/28/2001 11/29/2001 11/30/2001 12/09/2001 12/10/2001 12/11/2001 12/12/2001 12/13/2001 12/14/2001 Page 17 ofl05 March 12, 1998-November 5, 2010 PCL-401 mg/L Copolymer 0.009 0.028 0.009 0.009 0.009 0.009 0.009 0.019 0.019 0.018 0.019 0.019 0.019 0.019 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.021 0.021 0.021 0.020 0.021 0.020 0.02 0.019 0.019 0.02 0.02 0.02 0.02 0.02 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall101)
: 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion
Page 13 of 105
: Mollusks, During Toxicity Test Sampling, Date 10/07/2002 10/08/2002
 
' 10/09/2002 10/10/2002 10/11/2002 01/12/2003 01/13/2003 01/14/2003 01/15/2003 01/16/2003 01/17/2003 003 04/07/2003
REFERENCES
. 04/08/2003 04/09/2003 04/10/2003
: 1. NPDES Permit No. TN0026450.
: 2. USEP A. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
: 3. Standard Methods for the Examination of Water and Wastewater. 21st Edition, 2005.
: 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 ofl05
 
Sequoyah Nuclear Plant Biomonitoring November 2-9, 2010 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of105


04/11/2003 Page 18 of105 March 12, 1998-November 5, 2010 Table B-1 (continued).
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- November 5, 2010 Date                                           PCL-401 mg!L Copolymer 0.005 0.011 0.021 0.019 O.DlS O.DlS O.DlS 08/18/1999                                        0.024 08/19/1999                                        0.024 08/20/1999                                        0.024 08/21/1999                                        0.024 08/22/1999                                        0.024 08/23/1999                                        0.024 08/24/1999 '                                      0.023 Page 16 of 105
Sequoyah Nuclear Plant Diffuser (Outfall101)
Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, Date 08/04/2003 08/05/2003 08/06/2003 08/07/2003 08/08/2003 10/05/2003


10/06/2003 10/07/2003 10/08/2003 10/09/2003 10/10/2003 02/02/2004 02/03/2004 02/04/2004 02/05/2004 l.?f.\\'W\'\0;\'q,z,'.
Table B-1. Sequoyah Nuclear Plant Diffuser (OutfalllOl) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- November 5, 2010 Date                                          PCL-401 mg/L Copolymer 0.009 0.028 0.009 0.009 0.009 0.009 0.009 0.019 0.019 0.018 0.019 0.019 0.019 0.019 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.021 0.021 0.021 0.020 0.021 0.020 11/26/2001                                          0.02 11/27/2001 .                                      0.019 11/28/2001                                        0.019 11/29/2001                                          0.02 11/30/2001                                          0.02 12/09/2001 12/10/2001 12/11/2001 12/12/2001                                          0.02 12/13/2001                                          0.02 12/14/2001                                          0.02 Page 17 ofl05
02/06/2004 05/04/2004 05/05/2004 05/06/2004 05/07/2004 05/08/2004 05/09/2004 07/04/2004 07/05/2004 07/06/2004 07/07/2004 07/08/2004 07/09/2004 Page 19 of 105 March 12, 1998-November 5, 2010 PCL-401 mg/L Copolymer 0.020 0.014 0.014 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.022 0.024 0.009 0.009 0.009
 
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- November 5, 2010 Date 10/07/2002 10/08/2002 '
10/09/2002 10/10/2002 10/11/2002 01/12/2003 01/13/2003 01/14/2003 01/15/2003 01/16/2003 01/17/2003 003 04/07/2003 .
04/
* bve, D
* bve, D
* dead), SB
* dead),
* split brood (smgle brood split between two clays), CO
                                                          }<..      _;><...        ><-
SB
* split brood (smgle brood split between
                                                                                                    ~          "'><..        'fl'-            "><-        ><-          "::><..
two clays), CO
* oany over (oftipring oamed over With edult during transfer).
* oany over (oftipring oamed over With edult during transfer).
Concentration:  
                                                                                                                                                                                    )<..
% Mortality:
Concentration:
I 01 Mean Offspring/Female:
                                                                                                                        % Mortality:                                       I 01 Mean Offspring/Female:                             I "2.q,.t CONC:       11.3%                                                                   Survival and Reproduction Data Reolleate number Day                                             1             l             3             4           5           6             7           8               9         10 1         Young produeed                     0               0           0           0           0           0               0           0             6           0 Adult mortality                   \.....       L-         \.....           \.._           \.._         L             L..           L             \.._     L.
I "2.q,.t CONC: 11.3% Survival and Reproduction Data Reolleate number Day 1 l 3 4 5 6 7 8 9 10 1 Young produeed 0 0 0 0 0 0 0 0 6 0 Adult mortality  
l         Young produeed                     c             0             0           0           0           0             0           a             0           . 0' Adult mortality                 '--           L._           L.         L..         '-         \.....         '--             '-- \.....               \......
\..... L-\..... \.._ \.._ L L.. L \.._ L. l Young produeed c 0 0 0 0 0 0 a 0 .. 0' Adult mortality  
3         Young produced                   0             b             ('\         (")           0         CJ               0         0           0             0 Adult mortality                   L           L..           \......     L           L             L.             L           L               L           '--
'--L._ L. L.. '-\..... '--'--\..... \...... 3 Young produced 0 b ('\ (") 0 CJ 0 0 0 0 Adult mortality L L.. \...... L L L. L L L '--4 Young produeed  
4         Young produeed                   \4.         ~                            ""\           '-\           ~          '-\             ~            ~          '-\
\4. "" ""\ '-\ '-\ '-\ Adult mortality  
Adult mortality                 \....         '-           ""
\.... '-L. \..... \...... '-L. L... \.._ '-5 Young produced \C to 11. 1\ I '"'2-\ \ l'b a{) 10 Adult mortality  
L.         \.....       \......       '-           L.           L...             \.._     '-
'--\._ '--L '-'--'--L. \.._ L 6 Young produeed 0 () (") () 0 () 0 0 0 a Adult mortality  
5         Young produced                   l~          \C             to           11.           1\         I '"'2-           \ \       l'b           a{)         10 Adult mortality                   '--         \._                         L       '-             '--             '--         L.             \.._       L 6         Young produeed                   0             ()             (")           ()         0             ()           0           0             0           a Adult mortality                   '--           '-- '--                                                           \.._          '--           L..        '--
'--'--'--\.......
                                                                                                \.......      '--         '--
'--'--\.._ '--L.. '--7 Young produced \\ *'-llD 11 tc& 1'-( Total young produced 31.. 31. ao 32.. .31. 2.'i 30 Final Adult Mortality  
                                                                                                                                              ~~
-'-L. '-'-'---'-"-'--'--'--Note: Adult mortality (L = live, D
                                                        '~
7         Young produced                                 \\                                       ~~                                        tc&
                                                                                                                                                                                    *~
llD                       11                                       1'-(
Total young produced 31..         31.           ao             32..         2~          .31.         ~              ~3>          2.'i         30 Final Adult Mortality                           -'-           L.         '-           '-           '--         -'-           "-           '--           '--           '--
Note: Adult mortality (L = live, D
* deed), SB
* deed), SB
* split brood (single brood split between two days), CO
* split brood (single brood split between two days), CO
* oa over ofl!lorina carried over with adult durina transfer).
* oa   over ofl!lorina carried over with adult durina transfer).
Concentration:  
Concentration:
% Mortality:  
                                                                                                                        % Mortality:                                             01.
: 01. Mean Offspring/Female:
Mean Offspring/Female:                               ao.t
ao.t % Reduction from Control-I:
                                                                                                                        % Reduction from Control- I:                           -3.'tl~
SOP A Tll -Exhibit A Tll.2, revision 04-01-09 39of105 Page 3 of7 Species:
SOP ATll - Exhibit ATll.2, revision 04-01-09 39of105
Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant. OutfalllOl Date: I Hit*IO coNe: 22.6% Survival and Reproduction Data Replicate number Day 1 l 3 4 5 6 7 8 9 10 1 Young produced Q_ D D () 6 0 0 0 0 0 Adult mortality  
 
'-'--\.....-'-'-'--\.... '--'-'---2 Young produced 0 () a 0 0 c 0 a D a Adult mortality  
Page 3 of7 Species: Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant. OutfalllOl                                                                               Date:               I Hit*IO coNe: 22.6%                                                                       Survival and Reproduction Data Replicate number Day                                             1           l           3             4           5             6               7             8           9           10 1         Young produced                   Q_           D             D             ()         6           0                 0         0           0           0 Adult mortality                 '-           '--           \.....-       '-         '-               '--           \....         '-- '-               '---
'-\.......  
2         Young produced                     0             ()         a               0           0           c               0             a D                       a Adult mortality
'-l_ '--'-L. '--\.... \.......
                                                                    '-         \.......       '-           l_         '--           '-               L.             '--         \....     \.......
3 Young produced 0 a 0 D D a 0 a 6 a Adult mortality  
3           Young produced                     0           a               0           D             D             a             0               a         6           a Adult mortality
'-L... \...... L '--'-L. \-'--'--4 Young produced  
                                                                    '-           L...         \......     L             '--           '-             L.               \-         '-- '--
'--\ "'\ '-\ '-\ '-\ '-'""' 5 '-\ Adult mortality L-'--'--\..;... \_ \..... \..... \..... '-'"-5 Young produced 10 \"L. ,,_ \'\ 10 lO 10 1\ Adult mortality  
4         Young produced                     '--\       "'\             '-\         ~          '-\           '-\             '-                       5         '-\
\.. \.... '--'-'--'--L.. \..._ \..... \...... 6 Young produced  
Adult mortality                   L-         '--           '--           \..;...
(:) 0 c c 0 0 0 0 c c Adult mortality L-L '--'--'--\...._ '--\...... '-'---7 Young produced lq IS '" l& ,, tS \c& \t Total yonng produced a.\  
                                                                                                          ,,_            \_           \.....       \.....       '\.....
:!>\ '2.4\
5         Young produced                   ~,_
Final Adult Mortality  
10           \"L.                     \'\             l~              10             lO         10           1\
'-\.... '--'-\..... \.-. '--'-\.... '--Nolo: Adult monality (L
Adult mortality                     \..       \....       '--           '-       '--             '--             L..           \..._         \.....     \......
6         Young produced                   (:)         0             c             c             0           0               0             0           c           c Adult mortality lq L-         L           '--
l&
                                                                                                                                    ,,\...._
tS
                                                                                                                                                        '--             \...... '-         '---
                                                                                                                                                                                              \t
                                                                                                                                                                      '2*~
7         Young produced                               IS                                                                                                 \c&
Total yonng produced
                                                                ~s            '2.~          '"
                                                                                            ~1.          '!>>~
                                                                                                                      *~a.\         J~'-t            :!>\             .4\     ~b            '!>>~
Final Adult Mortality                             '-             \....       '--           '-         \.....       \.-.           '--               '-         \....     '--
Nolo: Adult monality (L
* live, D
* live, D
* SB
* dead~ SB
* split brood (single brood split between two days). CO
* split brood (single brood split between two days). CO
* c1111 over 'oflillriruz carried over with aduh durina transfer.
* c1111 over 'oflillriruz carried over with aduh durina transfer.
Concentration:  
Concentration:
% Mortality:
                                                                                                                                  % Mortality:                                             Ci1.
Ci1. Mean  
Mean Offsprin~emale:                                  ~&.'\
%Reduction from Control-1:  
                                                                                                                                  %Reduction from Control-1:                             *1.07..
*1.07 .. CONC: 45.2% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 ("\ 0 0 0 0 0 0 a a Adult mortality  
CONC:       45.2%                                                                 Survival and Reproduction Data Replicate number Day                                             1           2             3             4           5             6               7             8         9           10 1         Young produced                     0           ("\         0             0           0             0                 0 L
'-L-\..... '-\.,_ L L \.._ L '--2 Young produced 0 0 0 0 0 0 0 0 0 Adult mortality L L L L '-'-L. \_ L L 3 Young produced 0 0 c 0 0 a a 0 0 0 Adult mortality  
0           a         a Adult mortality
'-l_ L. l_ '--L.. L \...... L L-4 Young produced  
                                                                  '-           L-           \.....         '-         \.,_             L                             \.._       L 2         Young produced                     0           0           0               ~          0           0                 0             0           0       0 Adult mortality                     L             L           L           L               '-           '-               L.       \_           L           L 3         Young produced                     0         0             c               0         0               a             a               0           0         0 Adult mortality                     '- l _                  L.             l_         '--           L..               L           \......     L           L-4         Young produced                   '-\           s           '-\.         '-\         ~            "-\                 ~              ~        ~            ~
'-\ s '-\. '-\ "-\ Adult mortality  
Adult mortality                   '\...._ L.                             \.....     \......          \......           L          \......       L..         L 5
'\...._ L. \..... \..... \...... \...... L \...... L.. L 5 Young produced
                                                                                            \.....
'"" 1\ n \\ \"'1.. l"b \"Z.. l() 10 ,,_ Adult mortality L. \..... '-'--'---L \..... \.... L '-6 Young produced
n             \\                         l"b             \"Z..                     10           ,,_
_Q c c 0 0 Q_ 0 c c 0 Adult mortality L L '-'--'--\.... '--'--\.... l.... 7 Young produced 2.0 li tl. li{ \Qo I '"a \<<& ,, ,, Total young produced
Young produced                                  1\                                      \"'1..                                      l()
: 31. 3"\ 31o 3"\ Final Adult Mortality L L. '--'--\, '--'-\.... '-'--Note: Adult mortality (L
Adult mortality                   L.         \.....       '-             '--         '---             L             \.....       \....     L             '-
6         Young produced                   _Q           c           c               0         0               Q_               0             c           c           0 7
Adult mortality Young produced Total young produced L
2.0          li L           '-
tl.
li{          \Qo
                                                                                                                                        \....
I '"a
                                                                                                                                                      \<<&
                                                                                                                                                                      '~
                                                                                                                                                                                  \....       l....
: 31.         3"\           ~\            ~~            ~'-          31o             ~1.          ~              3"\         0~
Final Adult Mortality                               L           L.         '--           '--       \,
Note: Adult mortality (L
* live, D
* live, D
* dead), SB =split brood (single brood split between two CO= cs over of&orina carried owr with adult durina transler  
* dead), SB =split brood (single brood split between two days~ CO= cs
. Concentration:  
                                                                                                                                        '--              '-          \....      '-
% Mortality:  
over of&orina carried owr with adult durina transler .
: 01. Mean  
Concentration:
!&.b %Reduction from Control-1:  
                                                                                                                                  % Mortality:                                             01.
""12..&1.
Mean Offsprin~emale:                                    !&.b
SOP A Tll -Exhibit A Tll.2, revision 04-01-09 40 of 105 n I 'I I y;, I ;, \ .}'age 41 of 105 Page 4 of7 Species:
                                                                                                                                  %Reduction from Control-1:                             ""12..&1.
Ceriodauhnia dub/a Client: TVA I Seguoyab Nuclear Plant. OutfalllOl Date: n-cn.-10 CONe: 72.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced D D D ("') C) () 0 0 0 0 Adult mortality
SOP ATll - Exhibit ATll.2, revision 04-01-09
'-'-'--\...... \...-L... '--L L '-2 Young produced  
~,.age 40 of 105
("') 0 0 Q Q_ a_ _Q 0 0 a Adult mortaHty L L-'-'--'--'-L.. L.... '--'--3 Young produced 0 0 0 Q c D c _&#xa3;)_ C) 0 Adult mortality L. L. '-'--'--'-L '-'-'--4 Young produced "5!. "-\ '-'-\ '-\ '{ ..s Adult mortality
 
'-'-\..-'--'-L L L.. '--'--5 Young produced L"2-tO tb Lo l"L. \'Q 1\ H 'o Adult mortality L. '-'-'-''-'-\.... L. '-'--6 Young produced D 0 0 c 0 0 0 0 0 0 Adult mortality L '-'--'--L.... \..._ '--'--\..... 7 Young produced
      ~-
., ,, lt. 2.0 ,,. I&' ,, l\ '" \'\ Total young produced !>\ .:!>1. Final Adult Mortality  
    ~                                                                                                                                                                                Page 4 of7 n
\ '--'-'-'-'--'-'-\... Not*: Adult mortality (L
Species: Ceriodauhnia dub/a I~
Client: TVA I Seguoyab Nuclear Plant. OutfalllOl CONe:     72.6%                                                                 Survival and Reproduction Data Replicate number Date:                n-cn.-10
'I I
I y;,
Day 1         Young produced Adult mortality D
1 D
2 D
3            4
("')
                                                                                                            \......
5 C)
                                                                                                                          \...-
()
6 L...
0 7
                                                                                                                                                      '--       L 0
8 0
L 9
0 10 2         Young produced                   ("')       0             0             Q           Q_           a_         _Q             0             0           a Adult mortaHty               L               L-           '-             '-- '--                 '-           L..             L....       '--           '--
3         Young produced                     0             0           0             Q           c         D             c             _&#xa3;)_           C)         0 Adult mortality                 L.           L.           '- '--                     '--           '-             L         '-             '-           '--
4         Young produced Adult mortality "5!.       "-\         ~              ~            '-           '-\           '-\         '{             ~            ..s
                                                                    '- '-                     \..-           '--         '-               L           L           L..             '--       '--
5         Young produced               L"2-           tO           tb           Lo         l"L.         \'Q           1\           H               'o           \~
Adult mortality                 L.           '-             '-           '-         ''-         '-             \....       L.           '-         '--
6         Young produced                   D           0         0               c           0             0           0             0               0           0 Adult mortality Young produced                  ., ,,
L             '-         '--             '--
                                                                                                                        ,,.L....       ~
I&'          ,,
                                                                                                                                                    \..._       '--
l\
                                                                                                                                                                                '--         \.....
                                                                                                                                                                                                \'\
7                                                                   lt.           2.0 Total young produced
                                                                ~'\            !>\         ~\            ~a          ~\o.          ~~            .:!>1.         ~"\            ~\o        ~1 Final Adult Mortality                             \           '--           '-           '-           ~            '-             '--           '-           '-       \...
Not*: Adult mortality (L
* five, D
* five, D
* dead), SB-split brood (single brood split between two days), CO
* dead), SB- split brood (single brood split between two days), CO
* can over over with adult durina transfior.
* can over   o~ngcarried over with adult durina transfior.
Concentration:  
Concentration:
% Mortality: Mean Offspring/Female: % Reduction from Control-!:  
                                                                                                                                  % Mortality:                                             07~
-* "*'11 .. CONC* 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 D D 0 0 0 c a 0 Adult mortality  
Mean Offspring/Female:                               ~.~
'\.......
                                                                                                                                  % Reduction from Control-!:
L-L L L... \...._ \...... L L _l. 2 Young produced 0 c 0 0 D 0 0 0 0 0 Adult mortality  
                                                                                                                                                                                        -* "*'11..
\._ '-L '-L. '--L L "--L 3 Young produced 0 a 0 0 () D 0 0 0 0* Adult mortality  
CONC*     100%                                                                   Survival and Reproduction Data Replicate number Day                                           1             2             3             4           5           6             7           8             9           10 1         Young produced                     0           0             D           D           0             0               0           c             a         0 Adult mortality                   '\.......     L-         L           L               L...         \...._         \...... L               L         _l.
\._. \...... '-\...-'--L L \...... '--L 4 Young produced '-\. s '-\_ 'A "( Adult mortality  
2         Young produced                 0             c               0             0             D         0             0             0             0         0 Adult mortality                                                                                                     L                          "--        L
\.-\... \..... '-'--\._ '--\,.__ '--L 5 Young produced
                                                                \._
*'4 \\ \ "L L'l.. \"1. \ \ tD Lo \1.... Adult mortality  
                                                                                '-         L               '-           L.           '--                         L 3         Young produced                   0             a           0             0             ()           D             0             0             0           0*
'-\..._ '--'-\..... \.._ '-'-'-L 6 Young produced c c () c 0 c 0 0 0 0 Adult mortality L \._. '-'-L.. '-'-\,_ '-7 Young produced 2.0 '" 1'1 \'i 1.\ ll\ 1'\ 11 Total young produced "alit 3'-\ "!Q Final Adult Mortality \.. \. L '-L. L L '-\.._ Not*: Aduk mortality (L*Iive, D = daad), SB
Adult mortality                   \._.         \......     '-           \...-       '--             L             L         \......       '--           L 4         Young produced                   ~            '-\.         ~            s           '-\_         ~
                                                                                                                                      \._
                                                                                                                                                    ~            ~              'A           "(
Adult mortality                   \.-         \...         \.....       '-           '--                       '--             \,.__
                                                                                                                                                                                  '--           L
                                                                *'4                                                                                             Lo
                                                                                                                                                                                              *~L 5        Young produced                                \\           \ "L         L'l..       \"1.           \ \           tD                         \1....
Adult mortality
                                                                  '-               \..._       '--           '-         \.....       \.._
6         Young produced                   c             c           ()           c             0             c           0             0               0           0 Adult mortality                 ~            L           \._.
                                                                                                          '-           '-             L..           '- '-                   \,_
i~
                                                                                                                                                                                              '~
2.0
                                                                                              '"           1'1         \'i           1.\             ll\       1'\           11 7        Young produced Total young produced "alit         ~$          ~f..          ~\c
                                                                                                                      ~"
                                                                                                                                      ~,          3'-\         "!Q             ~~            ~Co Final Adult Mortality                             ~            \..       \.             L           '-           L.           L             L               '-         \.._
Not*: Aduk mortality (L*Iive, D = daad), SB
* spfit brood (single brood split between two days), CO
* spfit brood (single brood split between two days), CO
* ca over otfsorina carri<d over with aduk durin11 tralllfioi  
* ca     over otfsorina carri<d over with aduk durin11 tralllfioi .
. Concentration:  
Concentration:
% Mortality:
                                                                                                                                  % Mortality:                                           Cl1 Mean Offspring/Female:                               *'-<.t
Cl1 Mean Offspring/Female:  
                                                                                                                                  % Reduction from Control-!:                           -2o.l7.
*'-<.t % Reduction from Control-!:  
SOP ATil - Exhibit ATl1.2, revision 04-01-09
-2o.l7. SOP A Til -Exhibit A Tl1.2, revision 04-01-09
      \
. f ' :.i. i I I I I I I I I I I I I I ,Page 42 ofl05 Page 5 of7 Species:
  .}'age 41 of 105
Ceriodaphnia dub/a Client: TVA I Seguoyah Nuclear Plant. Outfall lOt Date: 1\ *0"1-10 CONTROL-2  
 
, Survival and Reproduction Data Replicate number Day 1 2 3 " s 6 7 8 9 10 1 Young produced a D a 0 c 0 c _Q_ _Q_ c Adult mortality L-\,.. '-'-\... '--'--\..... '-2 Young produced 6 0 ('") 0 0 D (') 0 0 ("') Adult mortality L-L. L L-\..... \.._ \.... L.. \.... \,_ 3 Young produced D 0 c 0 0 0 0 0 0 () Adult mortality
        .~
'-'-'-'-\......_
ff Page 5 of7
L L L... L L. 4 Young produced  
:.i.
'4 _:!) "\ '--\. _"i '-\ Adult mortality  
i               Species: Ceriodaphnia dub/a Client: TVA I Seguoyah Nuclear Plant. Outfall lOt CONTROL-2                                                                  , Survival and Reproduction Data Date:           1\ *0"1-10 I                Day                                              1          2 Replicate number s                                                                     10 1         Young produced                   a           D             a 3
'-'--\.... '--'--'-L-'--'--'--s Young produced JD \!>> l(:) lt:> 1 \ lt:;) \"t \0 \0 I() Adult mortality L. '--L-'-'--'-'--L-'-'-6 Young produced  
0 "              c 6
(') 6 c ("') 0 0 0 c 0 0 Adult mortality  
0           c 7              8
\...... '--'--'--'--\,_ '--'--L... 7 Young produced rS \S ,, \'-\ \'-\ L\o h ... \'$ ,., 11 Total young produced '0\ "28 'Ut 2.., 3\ Final Adult Mortality
_Q_         _Q_
'-'--'--'-\ '--\.... '--lo..... '--X for 3ra Broods X:: X. )<... >'-')<... :;><. >'-)<:. ')C. Nou. Adult monality (L
9 c
I                    2 Adult mortality Young produced L-6          0
                                                                                \,..
('")        0
                                                                                                                    \...
0 D
(')
                                                                                                                                                                \.....
0 0
                                                                                                                                                                                          ~
("')
Adult mortality                   L-         L.         L               L-           \.....         \.._           \....       L..         \....     \,_
I                    3         Young produced Adult mortality D
0             c 0         0
                                                                                                                    \......_
0 L
0 L
0 L...
0 L           L.
()
4         Young produced                   '4       _:!)             ~          "\             ~            '--\.         ~          _"i             '-\       ~
I                  s Adult mortality Young produced                  JD
                                                                          \!>>
                                                                              '--           \....
l(:)
lt:>
1\             lt:;)
                                                                                                                                                \"t L-        '--
                                                                                                                                                                \0
                                                                                                                                                                              \0         I()
Adult mortality                   L.           '--         L-           '-       '--               '-           '--         L-           '-         '-
I                  6         Young produced                   (')         6             c           ("')           0             0             0             c 0           0 7
Adult mortality Young produced                rS
                                                                  \......   '--
                                                                          \S           ,,
                                                                                                      \'-\
                                                                                                                  ~
                                                                                                                  \'-\         L\o
                                                                                                                                    '--          \,_
h...       \'$
                                                                                                                                                                '--          '--          L...
11 I              Total young produced Final Adult Mortality
                                                              '2.~
                                                                            ~\
                                                                                        '0\ "28
                                                                                                                    'Ut
                                                                                                                    \
                                                                                                                                  ~c
                                                                                                                                                ~'!.
                                                                                                                                                  \....
2..,
3\
lo.....
                                                                                                                                                                                          ~\
X for 3ra Broods                                   X::       X.             )<...       >'-           ')<...       ~            :;><.         >'-           )<:.       ')C.
I              Nou. Adult monality (L
* Uve, D =dead), SB
* Uve, D =dead), SB
* split brood (single brood spltt between two days), CO
* split brood (single brood spltt between two days), CO
* cany over (offspring earned over wtth aduh dunns tranSier).
* cany over (offspring earned over wtth aduh dunns tranSier).
Concentradon:  
Concentradon:
% Mortality:
                                                                                                                              % Mortality:                                     l C\1 ..
l C\1 .. Mean Offspring/Female:
I             CONC:       100% Intake Mean Offspring/Female:
I CONC: 100% Intake Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 D c 0 0 0 0 a 0 Adult mortality  
Survival and Reproduction Data I ~.l Replicate number I                Day 1         Young produced 1
'-L L.. L. L L L. L.. L L_ 2 Young* produced 0 0 (} 0 D 0 _Q 0 0 a Adult mortality
0         0 2
'-'--\.... L-L. L \...... L.. '--L 3 Young produced 0 D () 0 0 0 0 0 0 C; Adult mortality  
D 3
'-L. L _\. '-L L L \...._ \.-4 Young produced
c 4
"-l "t 'i s "" s '5 Adult mortality  
0 5
\..... L. '--'--'--\..... '--'--'--'--5 Young produced l\ \'L \ "L I_Q_ " 1\ \ \ 1'0 10 Adult mortality
0 6
\.......  
0 7
'-'-"--'--'--\..... '--\.... \...... 6 Young produced c 0 0 c 0 c c 0 0 0 Adult mortality L L \._ \..... L L \,__ \.......
0 8
L \.......
a 9          10 0
7 Young produced 11 ,, lS l't t'\ "2.0 la LS lR Total young produced 3t'2.. 2.& Final Adult Mortality  
Adult mortality
'-\..... '--\.... \....... '-'-'-L Nolt: Adult mortality (L = Uve, D
                                                                '-       L             L..             L.           L             L             L.         L..           L           L_
I                  2         Young* produced Adult mortality 0
0               (}
                                                                                          \....
0 L-D L.           L 0        _Q
                                                                                                                                              \......
0 L..
0
                                                                                                                                                                            '--         L a
3           Young produced                   0         D             ()             0           0             0           0             0           0           C; I                  4 Adult mortality Young produced
                                                                "-l L.
                                                                            ~
L "t
_\.
                                                                                                      'i s
L           L s
L
                                                                                                                                                              '5          ~
                                                                                                                                                                            \...._     \.-
                                                                                                                                                                                            ~
Adult mortality                 \.....         L.             '-- '--                 '--           ""
                                                                                                                                  \.....       '--         '--             '--       '--
I                  5         Young produced Adult mortality l\
                                                              \.......
                                                                          \'L
                                                                                          -~
                                                                                                      \ "L I_Q_
                                                                                                                  '--           "'--c 1\
                                                                                                                                                  \.....
                                                                                                                                                              \
                                                                                                                                                                  \        1'0
                                                                                                                                                                                \....
10
                                                                                                                                                                                          \......
6         Young produced                   c           0           0             c         0                             c             0           0         0 I                  7 Adult mortality Young produced                  11 L
L lS
                                                                                            \._       \.....
l't L
t'\
L "2.0
                                                                                                                                              \,__
la
                                                                                                                                                              \.......
                                                                                                                                                              \~            LS L         \.......
lR
                                                                                                                                            ~
Total young produced 3t'2..       ~              2.&         ~s          ~              ~5                            ~\          ~i2.        ~
~              Final Adult Mortality                           '-           \.....       '--           \....     \.......
Nolt: Adult mortality (L = Uve, D
* dead), SB
* dead), SB
* sp6t brood (sinal* brood spHt between two days). CO
* sp6t brood (sinal* brood spHt between two days). CO
* ca over olliorina carried over with adult durina trans!Or  
* ca
. Concentration:  
                                                                                                                                  ~              '-            '-            '-
% Mortality:  
over olliorina carried over with adult durina trans!Or .
: 01. Mean Offspring/Female:
Concentration:
3t*1 %Reduction from Control-2:  
L
-'*"1* SOP A Til -Exhibit A Tll.2, revision 04-0 l-09 t: """ .ETS * * * @
                                                                                                                              % Mortality:                                           01.
...... .,;,_..,'.'*'
Mean Offspring/Female:                               3t*1
!.) Environmental Tesllng Solutions, Inc. Control-1 Day 1 2 3 I 0 0 0 2 0. 0 0 3 0 0 0 4 4 4 4 5 11 13 10 6 0 0 0 7 15 15 15 Total 30 32 29 11.3% Day I 2 3 I 0 0 0 2 0 0 0 3 0 0 0 4 4 5 4 5 13 10 10 6 0 0 0 7 15 l7 16 Total 32 32 30 22.6o/o Day I 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 4 4 4 5 12 10 12 6 0 0 0 7 19 15 16 Total 35 29 32 45.2% Day I 2 3 I 0 0 0 2 0 0 0 3 0 0 0 4 4 5 4 5 12 11 11 6 0 0 0 7 20 18 16 36 34 31 TV A I Sequoyah Nuclear Plant, OutfalllOl
                                                                                                                              %Reduction from Control-2:
-Non-treated November 02-09, 2010 Verification of Cerlodaphnia Reproduction Totals 72.6% Replicate number Total 4 5 6 7 8 9 10 Day Replicate number I 2 3 4 5 6 7 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 3 4 4 4 5 4 4 40 4 5 4 5 5 6 4 4 13 10 12 II II 10 11 II2 5 12 10 10 13 12 13 11 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 14 14 16 13 15 14 15 146 7 17 17 16 20 18 18 17 30 28 32 28 31 28 30 298 Total 34 31 31 38 36 35 32 100% Reolicate number Total 4 5 6 7 8 9 10 Day Replicate number I 2 3 4 5 6 7 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 4 3 4 5 5 4 42 4 6 4 5 5 4 5 5 12 11 12 II 13 10 10 112 5 14 11 12 12 12 II 10 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 16 l3 17 15 15 14 16 154 7 19 20 19 19 18 21 19 32 28 32 30 33 29 30 308 Total 39 35 36 36 34 37 34 Control-2 Re number Total l 4 5 6 7 8 9 10 Day Replicate number I 2 3 4 5 6 7 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 5 4 4 6 4 5 4 44 4 4 3 4 4 3 4 5 12 II 13 10 10 10 II III I 5 10 13 10 10 11 10 12 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 18 16 17 15 15 15 18 164 7 15 15 17 14 14 16 16 35 31 34 31 29 30 33 319 Total 29 31 31 28 28 30 33 100% Intake RePlicate number Total 4 5 6 7 8 9 10 Day Replicate number I 2 3 4 5 6 7 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 6 4 5 5 5 4 46 4 4 4 4 4 5 4 5 11 12 13 12 10 10 12 114 5 11 12 9 12 10 11 11 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 19 18 19 15 15 19 l7 176 7 17 17 15 19 19 20 18 34 36 36 32 30 34 33 336 Total 32 33 28 35 34 35 34 8 9 10 Total 0 0 0 0 0 0 0 0 0 0 0 0 4 4 5 46 11 13 13 118 0 0 0 0 19 19 19 180 34
SOP ATil - Exhibit ATll.2, revision 04-0 l-09
.._344. 8 9 10 Total 0 0 0 0 0 0 0 0 0 0 0 0 6 4 4 48 13 12 13 120 0 0 0 0 19 17 19 190 38 33 36 358 8 9 10 Total 0 0 0 0 0 0 0 0 0 0 0 0 4 4 4 39 10 10 10 106 0 0 0 0 15 17 17 156 29 31 31 301 8 9 10 Total 0 0 0 0 0 0 0 0 0 0 0 0 5 4 5 44 11 13 10 110 0 0 0 0 15 15 18 173 31 32 33 327 
                                                                                                                                                                                    -'*"1*
"' ol::.. ol::.. ..... * * *
,Page 42 ofl05
* Environmental Testing Solutions, Inc. TVA I Sequoyah Nuclear Plant, OutfalllOl Non-treated November 02-09,2010 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project 6709 ---Concentration I 2 3 4 Control-!
 
30 32 29 30 ll.3% 32 32 30 32 22.6% 35 29 32 35 45.2% 36 34 31 34 72.6% 34 31 31 38 100% 39 35 36 36 Control-2 29 31 31 28 100% Intake 32 33 28 35 ---OutfalllOI:
~
MSD= Dunnett's MSD value: 2.057 PMSD= PMSD: 69 Intake: Dunnett's MSD value: 1.451 PMSD: 4.8 Keve*weo oy:
    .ETS TVA I Sequoyah Nuclear Plant, OutfalllOl -Non-treated
Replicate number Survival Average reproduction Coeftlcleat of Percent reductloa fram 5 6 7 8 9 10 (%) (offspring/female) nrlotloa
~
(%) control (%) 28 32 28 31 28 30 100 29.8 5.2 Not applicable 28 32 30 33 29 30 100 30.8 5.3 -3.4 31 34 31 29 30 33 100 31.9 7.2 -7.0 36 36 32 30 34 33 100 33.6 6.3 -12.8 36 35 32 34 36 37 100 34.4 7.1 -15.4 34 37 34 38 33 36 100 35.8 5.2 -20.1 28 30 33 29 31 31 100 30.1 5.3 Not applicable 34 35 34 31 32 33 100 32.7 6.5 -8.6 -Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision.
t:  **
The PMSD is the minimum percent difference between tbe control and treatment tbat can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (l<f' percentile)
November 02-09, 2010 Verification of Cerlodaphnia Reproduction Totals
= 13%. Upper PMSD bound determined by USEPA (91f' percentile)=
~  @ --:u.*;~**''    ......    .,;,_..,'.'*'
47%. Lower and upper PMSD bounds were determined from tbe 10tb and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200Ia; USEPA, 200lb). USEPA 2000. Understanding and Accounting for Metbod Variability in Whole Effluent Toxicity Applications Under tbe National Pollutant Discharge Elimination Program.
~
EPA-833-R-00-003.
~
US Environmental Protection Agency, Cincinnati, OH. USEPA 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Cbrooic and Acute Whole Effluent Toxicity Test Metbods, Volumes 1 and 2-Append.ix.
    !.) Environmental Tesllng Solutions, Inc.
EPA-821-B-01-004 and EPA-821-B-01-005.
Control-1                                                                                  72.6%
US Environmental Protection Agency, Cincinnati, OH. ! I i 
Replicate number                                                      Replicate number Day                                                                    Total          Day                                                Total 1    2         3 4     5     6   7   8  9  10                            I 2   3 4    5      6  7  8  9  10 I            0     0         0 0      0     0   0   0   0   0     0               I    0   0   0  0    0      0  0  0  0   0       0 2           0. 0         0 0      0      0   0   0   0   0     0               2    0  0  0  0    0      0  0  0  0  0      0 3            0      0        0  0      0      0    0 0   0     0     0               3    0   0   0 0     0     0   0 0 0   0       0 4           4      4        4  3      4      4   4   5   4   4     40               4     5 4   5 5     6     4   4  4  4   5      46 5          11    13       10 13    10      12 II II 10   11   II2             5     12 10 10 13   12     13 11 11 13  13    118 6            0     0         0 0     0     0   0   0   0    0      0              6     0  0  0  0    0     0   0 0 0   0       0 7          15    15        15 14     14     16 13 15 14   15   146             7     17 17 16 20   18     18 17 19 19  19    180 Total          30    32        29 30     28     32   28 31 28   30     298           Total 34 31 31 38   36     35 32 34 ~..]7  .._344.
..... TVA/ Sequoyah Nuclear Plant, Outfall 101 Non-treated
11.3%                                                                                      100%
*Ers * * * ""'".:;,;/ ,_.,, **,f'<'';,-
Reolicate number                                                       Replicate number Day                                                                    Total           Day                                                Total I      2        3  4     5     6   7 8   9   10                           I 2   3 4     5     6   7 8  9  10 I          0     0         0 0     0       0   0 0   0   0       0             I    0   0   0 0     0     0   0 0 0  0      0 2            0      0        0  0      0      0  0  0   0     0     0             2     0  0  0  0    0      0  0  0 0   0       0 3            0     0         0 0     0       0   0 0   0     0     0             3     0  0  0  0    0     0   0 0 0   0       0 4           4      5        4 4      4      3   4   5   5   4     42               4     6   4   5 5     4     5   5 6  4  4      48 5          13    10        10 12    11     12 II 13   10   10   112             5     14 11 12 12   12     II 10 13 12  13    120 6            0      0        0  0     0     0   0 0   0   0     0               6     0  0  0 0     0     0   0 0 0   0      0 7          15    l7        16 16     l3     17 15 15   14   16   154             7     19 20 19 19   18     21 19 19 17  19    190 Total          32     32       30 32    28    32  30 33 29   30     308           Total 39 35 36 36   34     37 34 38 33  36    358 22.6o/o                                                                                    Control-2 Re ~Jiicate number                 Total l                             Replicate number Day                                                                                    Day                                                Total I      2        3  4     5       6   7 8   9   10                           I 2   3 4     5     6   7 8  9  10 1          0     0         0 0     0       0   0 0   0   0     0             I      0  0  0  0    0      0  0  0  0  0     0 2            0     0         0 0     0       0   0 0   0     0     0              2     0  0  0  0     0     0   0  0 0   0       0 3            0     0         0 0     0       0   0 0   0    0      0              3     0  0  0  0     0     0   0 0 0   0       0 4            4     4         4 5     4       4   6  4    5    4     44              4      4  3   4 4     3     4  5  4 4  4       39 5           12     10        12 12    II     13 10 10   10   II   III   I         5     10 13 10 10   11     10 12 10 10  10    106 6            0     0         0 0     0       0   0 0   0    0      0              6     0  0  0  0     0     0   0 0 0   0       0 7          19    15        16 18     16     17 15 15   15   18   164             7     15 15 17 14   14     16 16 15 17  17    156 Total          35    29        32 35     31     34   31 29 30   33     319           Total   29 31 31 28   28     30 33 29 31  31    301 45.2%                                                                                      100% Intake RePlicate number                                                       Replicate number Day                                                                    Total           Day                                              Total I      2        3  4     5       6   7 8   9   10                           I 2   3 4     5     6   7 8  9  10 I          0     0         0  0      0      0  0  0   0   0     0             I     0  0  0  0     0      0  0  0  0   0       0 2          0     0         0 0     0       0   0 0   0   0     0             2     0  0  0  0    0      0  0  0 0   0       0 3          0     0         0 0     0       0   0 0   0   0     0             3     0 0   0 0     0     0   0 0  0  0      0 4          4      5        4  4      6       4   5 5   5   4     46             4     4 4   4 4     5     4   5  5 4  5      44 5          12    11        11 11     12     13 12 10 10   12   114             5     11 12   9 12     10   11 11 11 13  10    110 6          0      0        0  0     0       0   0 0   0   0     0             6     0 0   0 0     0     0   0 0  0  0      0 7          20    18        16 19     18      19  15 15   19   l7   176             7     17 17 15 19   19     20 18 15 15  18     173
;,) Environmental Testing Solutions,
          ~T'!!!!..,_        36    34       31 34    36     36 32 30   34   33   336           Total   32 33 28 35 34 35 34         31 32  33    327
.,c. November 02-09, 2010 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: ll/212010 TestiD: CdFRCR Sample 10: End Date: ll/912010 LabiD: ETS-Envir.
 
Testing Sol. Sample Type: Sample Date: Protocol:
~
FWCHR-EPA-821-R-02-013 Test Species:
~                                                                        TVA I Sequoyah Nuclear Plant, OutfalllOl ol::..
Comments:
ol::..                                                                                                  Non-treated
Non-treated Cone-% 1 2 3 4 5 6 7 8 Control-!
~                                                                                            November 02-09,2010
30.000 32.000 29.000 30.000 28.000 32.000 28.000 31.000 Control-2 29.000 31.000 31.000 28.000 28.000 30.000 33.000 29.000 11.3 32.000 32.000 30.000 32.000 28.000 32.000 30.000 33.000 22.6 35.000 29.000 32.000 35.000 31.000 34.000 31.000 29.000 45.2 36.000 34.000 31.000 34.000 36.000 36.000 32.000 30.000 72.6 34.000 31.000 31.000 38.000 36.000 35.000 32.000 34.000 100 39.000 35.000 36.000 36.000 34.000 37.000 34.000 38.000 Intake 32.000 33.000 28.000 35.000 34.000 35.000 34.000 31.000 Transform:
~
Untransformed Cone-% Mean N-Mean Mean Min Max CV% N t.Stat Control-!
* Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test
29.800 0.9900 29.800 28.000 32.000 5.199 10 Control-2 30.100 1.0000 30.100 28.000 33.000 5.299 10 11.3 30.800 1.0233 30.800 28.000 33.000 5.258 10 -1.112 22.6 31.900 1.0598 31.900 29.000 35.000 7.156 10 -2.334 45.2 33.600 1.1163 33.600 30.000 36.000 6.306 10 -4.224 72.6 34.400 Ll429 34.400 31.000 38.000 7.147 10 -5.ll3 100 35.800 Ll894 35.800 33.000 39.000 5.234 10 -6.670 Intake 32.700 1.0864 32.700 28.000 35.000 6.455 10 Auxiliary_
* ET~
Tests Statistic Kolmogorov D Test indicates normal distribution (p > 0.01) 0.720721424 Bartlett's Test indicates equal variances (p = 0. 71) 2.931964874 The control means are not significantly different (p = 0.67) 0.426640917 Hypothesis Test (!-tail, 0.05) N()EC WEC ChV TU MSDu MSDp Dunnett's Test 100 >100 1 2.057058738 0.069028817 Treatments vs Control-I Point % ICOS >100 IC10 >100 ICI5 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 so 95%CL Linear Interpolation (200 Resamples)
EPA-821-R-02-013, Method 1002.0 Quality Control
Skew TVA I Sequoyah Nuclear Plant, OutfalllOl DMR-Discharge Monitoring Report CD-Ceriodaphnia dubia 9 28.000 31.000 29.000 30.000 34.000 36.000 33.000 32.000 !-Tailed 10 30.000 31.000 30.000 33.000 33.000 37.000 36.000 33.000 Isotonic Critical MSD Mean N-Mean 2.287 2.287 2.287 2.287 2.287 Critical 1.035 15.08627224 2.100922029 2.057 2.057 2.057 2.057 2.057 MSB MSE 51.93666667 4.046296296 32.717 1.0000 32.717 1.0000 32.717 1.0000 32.717 1.0000 32.717 1.0000 32.717 1.0000 Skew Kurt -0.072330161
        ~      Environmental Testing Solutions, Inc.
-0.95920896 F-Prob df 3.IE-08 5, 54 sqnl01_110210dauz 
Verification of Data Entry, Calculations, and Statistical Analyses Project number~                                        6709 Keve*weo oy:    ~"v~
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Concentration                                                      Replicate number                                                      Survival    Average reproduction        Coeftlcleat of  Percent reductloa fram
'i*_,,..  
(*~)                                                                                                                                  (%)          (offspring/female)      nrlotloa (%)          control (%)
. .. Environmental Testing Solutions, Inc. Start Date: 111212010 End Date: 11/9/2010 Sample Date: Comments:
I          2            3          4           5            6            7              8    9          10 Control-!        30          32          29        30          28          32          28            31    28          30          100                29.8                   5.2          Not applicable ll.3%            32          32            30        32          28          32           30            33   29          30          100                30.8                  5.3                    -3.4 22.6%            35          29            32          35          31          34          31            29    30          33          100                31.9                  7.2                  -7.0 45.2%            36          34          31          34          36          36          32            30    34          33          100                33.6                  6.3                  -12.8 72.6%            34          31          31          38          36          35          32            34    36          37          100                34.4                  7.1                  -15.4           !
Non-treated Cone-% I 2 Control-I 30.000 32.000 Control-2 29.000 31.000 11.3 32.000 32.000 22.6 35.000 29.000 45.2 36.000 34.000 72.6 34.000 31.000 100 39.000 35.000 Intake 32.000 33.000 Cone-% Mean N-Mean Control-I 29.800 0.9900 Control-2 30.100 1.0000 I 1.3 30.800 1.0233 22.6 31.900 1.0598 45.2 33.600 1.1163 72.6 34.400 1.1429 100 35.800 1.1894 Intake 32.700 1.0864 TV A I Sequoyah Nuclear Plant, OutfalllOl
100%            39          35           36          36         34           37          34           38   33          36          100               35.8                  5.2                  -20.1          Ii Control-2         29           31           31         28         28          30          33            29    31          31          100                30.1                  5.3          Not applicable 100% Intake       32           33           28         35          34          35           34            31    32          33          100                32.7                    6.5                  -8.6 OutfalllOI:                                           MSD=         Minimum Significant Difference Dunnett's MSD value:         2.057                   PMSD=       Percent Minimum Significant Difference PMSD:                       69                                   PMSD is a measure of test precision. The PMSD is the minimum percent difference between tbe control and treatment tbat can be declared statistically significant in a whole effluent toxicity test.
-Intake Non-treated November 02-09, 2010 Statistical Analyses CeriodaJ!Imia Survival and Reproduction Test-R9!roduction TestiD: CdFRCR SampleiD:
Intake:
TV A I Sequoyah Nuclear Plant, Intake Lab ID: ETS-Envir.
Dunnett's MSD value:         1.451                                 Lower PMSD bound determined by USEPA (l<f' percentile) = 13%.
Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol:
PMSD:                       4.8                                   Upper PMSD bound determined by USEPA (91f' percentile)= 47%.
FWCHR-EPA-821-R-02-013 Test Species:
Lower and upper PMSD bounds were determined from tbe 10tb and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200Ia; USEPA, 200lb).
CD-Ceriodaphnia dubia 3 4 5 6 7 8 9 10 29.000 30.000 28.000 32.000 28.000 31.000 28.000 30.000 31.000 28.000 28.000 30.000 33.000 29.000 31.000 31.000 30.000 32.000 28.000 32.000 30.000 33.000 29.000 30.000 32.000 35.000 31.000 34.000 31.000 29.000 30.000 33.000 31.000 34.000 36.000 36.000 32.000 30.000 34.000 33.000 31.000 38.000 36.000 35.000 32.000 34.000 36.000 37.000 36.000 36.000 34.000 37.000 34.000 38.000 33.000 36.000 28.000 35.000 34.000 35.000 34.000 31.000 32.000 33.000 Transfonn:
USEPA 2000. Understanding and Accounting for Metbod Variability in Whole Effluent Toxicity Applications Under tbe National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Untransfonned 1-Tailed Mean Min Max CV% N t-Stat Critical MSD 29.800 28.000 32.000 5.199 10 30.100 28.000 33.000 5.299 10 30.800 28.000 33.000 5.258 10 31.900 29.000 35.000 7.156 10 33.600 30.000 36.000 6.306 10 34.400 31.000 38.000 7.147 10 35.800 33.000 39.000 5.234 10 32.700 28.000 35.000 6.455 10 -3.108 1.734 1.451 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01) 0.954421401 0.868 -0.721765822 0.887179168 F-Test indicates equal variances (p = 0.42) 1.751091719 6.541089535 The control means are not signi_!icantly different (p = 0.&sect;]) 0.426640917 2.100922029 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.450821719 0.048200057 33.8 3.5 0.006077496 I, 18 Treatments vs Control-
USEPA 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Cbrooic and Acute Whole Effluent Toxicity Test Metbods, Volumes 1 and 2-Append.ix. EPA-821-B-01-004 and EPA-821-B-01-005.
"' """' v. * * *
US Environmental Protection Agency, Cincinnati, OH.
* ETS !.'"*:7\
 
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~
Environmental Testing Solutions, Inc. Control 11.3% 11.6% 45.1% 71.6% 100% 100% Iutake Alkalinity (mgtL L.:aL.:U3) Harduess (mg/L CaC03) ----------TV A I Sequoyah Nuclear Plant, OutfalllOl
~                                                                          TVA/ Sequoyah Nuclear Plant, Outfall 101
-Non-treated November 02-09, 2010 Ceriodaphnia dubia Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Project number: 6709 r ... . . r . i r
~
* I I I I I I i T.c S""""' ,.,:} .. -*-*-* EmrlranmenttiTIICfngSalutlons,lnc.
Non-treated
Species:
      *Ers
Cer/odaphnla dub/a Client: TV A I Sequoyah Nuclear Plant. OutfalllOl CONTROL 11.3% 22.6% 45.2% 72.6% 100% 100% Intake age48 of lOS Page 6 of7 SOP A Tll -Exhibit A Tll.2, revision 04-01-09 I I I CONTROL 11.3%. 22.6% 45.2% 72.6o/o 100% 100% Intake Page 49 of 105 !nllllunmtfthiTntlngSalutfons.lnc.
~
Page 7 of7 Species:
November 02-09, 2010
Ceriodanhnia dub/a Client: TV A I Seguoyab Nuclear Plant, OutfalllOl Date:
~
SOP AT II -Exhibit A TI I.2, revision 04-0 I-09 I I I I I * * *
Statistical Analyses
* Chronic Whole Effiuent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species:
      ~      ""'".:;,;/ ~1      ,_.,,     **,f'<'';,-
Pimepha/es promelas Client: Tennessee Valley Authority Facility:
      ;,) Environmental Testing Solutions,            .,c.
Seauoyah Nuclear Plant NPDES #: TN0020168 Project #:
Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:        ll/212010                              TestiD:        CdFRCR                                        Sample 10:                TVA I Sequoyah Nuclear Plant, OutfalllOl End Date:          ll/912010                              LabiD:        ETS-Envir. Testing Sol.                       Sample Type:              DMR-Discharge Monitoring Report Sample Date:                                              Protocol:      FWCHR-EPA-821-R-02-013                        Test Species:             CD-Ceriodaphnia dubia Comments: Non-treated Cone-%                    1                     2             3            4              5                6              7          8                9          10 Control-!                  30.000              32.000        29.000      30.000          28.000          32.000        28.000    31.000          28.000    30.000 Control-2                  29.000              31.000        31.000      28.000          28.000          30.000        33.000    29.000          31.000    31.000 11.3                32.000              32.000        30.000       32.000         28.000           32.000        30.000     33.000           29.000     30.000 22.6                35.000             29.000         32.000      35.000          31.000           34.000         31.000     29.000           30.000     33.000 45.2                36.000             34.000         31.000       34.000         36.000           36.000         32.000     30.000           34.000      33.000 72.6               34.000             31.000         31.000       38.000         36.000           35.000         32.000     34.000           36.000     37.000 100                39.000             35.000         36.000       36.000         34.000           37.000         34.000     38.000           33.000     36.000 Intake                32.000             33.000         28.000       35.000         34.000           35.000         34.000     31.000           32.000     33.000 Transform: Untransformed                                             !-Tailed                            Isotonic Cone-%               Mean               N-Mean             Mean         Min             Max           CV%               N       t.Stat         Critical      MSD            Mean          N-Mean Control-!                 29.800             0.9900         29.800       28.000         32.000           5.199       10                                                       32.717        1.0000 Control-2                 30.100             1.0000         30.100       28.000         33.000           5.299       10 11.3               30.800             1.0233         30.800       28.000         33.000           5.258       10           -1.112           2.287      2.057          32.717        1.0000 22.6               31.900             1.0598         31.900       29.000         35.000           7.156       10           -2.334           2.287      2.057          32.717        1.0000 45.2               33.600             1.1163         33.600       30.000         36.000           6.306       10           -4.224           2.287      2.057          32.717        1.0000 72.6               34.400             Ll429         34.400       31.000         38.000           7.147       10           -5.ll3           2.287      2.057          32.717        1.0000 100               35.800               Ll894         35.800       33.000         39.000           5.234       10           -6.670           2.287      2.057          32.717        1.0000 Intake               32.700             1.0864         32.700       28.000         35.000           6.455       10 Auxiliary_ Tests                                                                                                           Statistic                 Critical                      Skew            Kurt Kolmogorov D Test indicates normal distribution (p > 0.01)                                                             0.720721424                     1.035                  -0.072330161    -0.95920896 Bartlett's Test indicates equal variances (p = 0. 71)                                                                   2.931964874               15.08627224 The control means are not significantly different (p = 0.67)                                                           0.426640917               2.100922029 Hypothesis Test (!-tail, 0.05)                               N()EC         WEC             ChV               TU           MSDu       MSDp             MSB        MSE            F-Prob          df Dunnett's Test                                                 100         >100                             1       2.057058738 0.069028817 51.93666667 4.046296296            3.IE-08          5, 54 Treatments vs Control-I Linear Interpolation (200 Resamples)
___ _ Dilution preparation information:
Point                     %                     so                  95%CL                  Skew ICOS                             >100 IC10                             >100 ICI5                             >100 IC20                             >100 IC25                             >100 IC40                             >100 IC50                             >100 sqnl01_110210dauz
Dilution prep (%) 11.3 22.6 Effluent volume (mL) 282.5 565 45.2 1130 Diluent volume (mL) 2217.5 1935 1370 Total volume (mL) 2500 2500 2500 Test orflanism information:
 
72.6 1815 685 2500 Organism age: 4S. "'\ 1-\ft" Mo. () \.b Date and times organisms I\'"(),.,
~
0 lloOO were born between:
~                                                                    TVA I Sequoyah Nuclear Plant, OutfalllOl - Intake Q\
100 2500 0 2500 Organism source: &\*tu4 Pr &\"0\*IC Transfer bowl information:
Non-treated
pH= 1.1, s.u. Temperature
~
=15.'1.!'C
~
. Average transfer volume:
t.JJ November 02-09, 2010
County: !Y!Y Outfall:
      .ETS
101 Comments:
      ~
Each concentration was UV -treated for 2 minutes to remove pathogenic Interferences.
            '"-''.'.<*:;/".;;,      'i*_,,..
Test information:
Environmental Testing Solutions, Inc.
Randomizing template:
                                                  ... :~'"''"
Incubator number and shelf location:
Statistical Analyses CeriodaJ!Imia Survival and Reproduction Test-R9!roduction Start Date: 111212010                                          TestiD:        CdFRCR                                      SampleiD:                    TVA I Sequoyah Nuclear Plant, Intake End Date:              11/9/2010                              Lab ID:        ETS-Envir. Testing Sol.                     Sample Type:                  DMR-Discharge Monitoring Report Sample Date:                                                  Protocol:      FWCHR-EPA-821-R-02-013                      Test Species:                CD-Ceriodaphnia dubia Comments: Non-treated Cone-%                          I                  2             3            4              5            6              7            8              9          10 Control-I                        30.000            32.000        29.000        30.000          28.000        32.000          28.000        31.000          28.000      30.000 Control-2                       29.000            31.000        31.000      28.000          28.000        30.000          33.000        29.000          31.000      31.000 11.3                  32.000            32.000        30.000      32.000          28.000        32.000          30.000        33.000          29.000      30.000 22.6                  35.000            29.000        32.000      35.000          31.000        34.000          31.000        29.000          30.000      33.000 45.2                  36.000            34.000        31.000      34.000          36.000        36.000          32.000        30.000         34.000       33.000 72.6                  34.000             31.000        31.000       38.000         36.000        35.000         32.000       34.000          36.000       37.000 100                  39.000            35.000         36.000       36.000         34.000       37.000         34.000       38.000          33.000      36.000 Intake                    32.000            33.000        28.000      35.000          34.000        35.000          34.000        31.000          32.000      33.000 Transfonn: Untransfonned                                            1-Tailed Cone-%                  Mean                  N-Mean          Mean          Min            Max            CV%              N          t-Stat        Critical      MSD Control-I                       29.800            0.9900        29.800      28.000          32.000        5.199        10 Control-2                        30.100            1.0000        30.100      28.000         33.000         5.299        10 I 1.3                  30.800            1.0233        30.800      28.000         33.000         5.258        10 22.6                    31.900            1.0598        31.900      29.000         35.000         7.156        10 45.2                    33.600            1.1163        33.600      30.000         36.000         6.306        10 72.6                  34.400            1.1429        34.400      31.000         38.000         7.147        10 100                  35.800            1.1894        35.800      33.000         39.000         5.234        10 Intake                    32.700            1.0864        32.700      28.000         35.000         6.455        10              -3.108          1.734        1.451 Auxiliary Tests                                                                                                              Statistic                    Critical                      Skew          Kurt Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01)                                                              0.954421401                      0.868                    -0.721765822  0.887179168 F-Test indicates equal variances (p = 0.42)                                                                              1.751091719                  6.541089535 The control means are not signi_!icantly different (p = 0.&sect;])                                                            0.426640917                  2.100922029 Hypothesis Test (1-tail, 0.05)                                                                                              MSDu          MSDp            MSB          MSE              F-Prob        df Homoscedastic t Test indicates no significant differences                                                                1.450821719 0.048200057            33.8        3.5          0.006077496      I, 18 Treatments vs Control-2 sqnl01_1102JO~a
' Artemia CHM number:
 
Drying information for weight determination:
~                                                                          TVA I Sequoyah Nuclear Plant, OutfalllOl - Non-treated
Date I Time in oven: 11-Nl*IO Initial oven temperature:  
~
!Db. t. Date I Time out of oven: 11-rb*IO Final oven temperature:
November 02-09, 2010
'-''(. Page l of6 IllS Total drying time:
~
I Daily feeding and renewal information:
~
I I I I I I I I Day Page 50 of 105 Date Morning reeding Afternoon reeding Test initiation,  
~
: renewal, or Sample numbers used MHSW batch SOP A T20 -Exhibit A T20.3, revision 04-01-09 
: v.
*' I iu ; : 1: ! u u IJ Page 2 of6 .;) Environmental Testing Solutions, Inc. Species:
* Ceriodaphnia dubia Chronic Whole Emuent Toxicity Test
Pimephales promelas
    ~
, Client: TV A I SeQuoyah Nuclear Plant, Outfall101.
    ~
UV-treated Date: 1\*0t*IO Survival and ,..._ *'-Data Day CONTROL 11.3% 22.6% UV-treated A B c D E F G H I J K L 0 ID ID to ID IO ID 10 10 ID 10 '0 10 1 /0 IO /0 I{) IO 10 /0 10 IO 10 10 2 ' IO IC /Q /Q IC I(J to /0 IO /Cj 10 10 3 lC IC It:> IO 10 lO 10 IO lt> 10 it) 10 4 10 /0 10 10 10 tO /0 to rc I() /Q fQ 5 I 0 IC f() 10 10 lO 10 IQ tO tD 10 ID 6 IO I'D LO 10 IC 10 10 IO lC It> 10 10 7 10 r'() /l) IQ *o 10 IC to /0 IO 10 tO' A=
ETS              !.'"*:7\      J.: <;::...:>:**.:*.,."**;:,,.
W.u.t ,_.'\0 \'-\!\1
Environmental Testing Solutions, Inc.
\?a .'tl
EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Project number: 6709 Control 11.3%
\'\.fb\
11.6%
[
45.1%
'L.lO B ' Pan+
71.6%
weight 'f rzt.Cft 1t\.f;t' lal15 Zf.'PC 21-=l-\
100%
111-!H llh_ld
100% Iutake Alkalinity (mgtL L.:aL.:U3)
.n. a o
Harduess (mg/L CaC03)
*--C = Larvae weight (mg) =B-A 1*"-1 ,,\.() &,7..\ '\.10 &.14\ '\,oR &.l.\. &.!tb &.2.1 a.c.t
                        --    --    - ---    -                      -    ~
[1.\\ Weight per initial number oflarvae (mg) ,,... ,...'-'0
 
.c) = C I Initial Dllmber of r# larvae (). Cl. (:)' t>' r:;,* lo' ()" o* b. Avenge *Percent weight per reduction o.&-o"l o. &Ia\ z.&7..  
r .
.. initial from number of control(%)
iE.~"~'" T.cS""""'
                  ,.,:}
                  ~
                        .. -*-        *-*      *~*,.
EmrlranmenttiTIICfngSalutlons,lnc.
Page 6 of7 Species: Cer/odaphnla dub/a Client: TVA I Sequoyah Nuclear Plant. OutfalllOl CONTROL r.
i r
*I 11.3%
22.6%
I 45.2%
I I                72.6%
I I                  100%
100%
Intake SOP ATll - Exhibit ATll.2, revision 04-01-09 age48 of lOS
 
              ~ETS Page 7 of7
              ~ !nllllunmtfthiTntlngSalutfons.lnc.
Species: Ceriodanhnia dub/a Client: TVA I Seguoyab Nuclear Plant, OutfalllOl                Date:    u~<D.*IO CONTROL 11.3%.
22.6%
I 45.2%
72.6o/o I
I      100%
100%
Intake SOP AT II - Exhibit ATI I.2, revision 04-0 I-09 Page 49 of 105
 
I
* Page l of6
          ~
Chronic Whole Effiuent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimepha/es promelas Client: Tennessee Valley Authority                                                        County: !Y!Y Facility: Seauoyah Nuclear Plant                                                          Outfall: 101 NPDES #: TN0020168 Project #: _....;:'-~1LlD.._~_.___ _ __
Dilution preparation information:                                               Comments:
Dilution prep (%)         11.3      22.6        45.2      72.6        100    Each concentration was UV -treated Effluent volume (mL)    282.5        565        1130      1815      2500    for 2 minutes to remove pathogenic Diluent volume (mL)    2217.5      1935        1370        685        0     Interferences.
Total volume (mL)        2500      2500        2500      2500      2500 I                  Test orflanism information:                                                    Test information:
Organism age:                    \~.4S."'\ 1-\ft" Mo. () \.b                  Randomizing template:     f)l~e..
I                  Date and times organisms were born between:
I\'"(),., 0    lloOO                        Incubator number and shelf location: '
                                                                                                                                ~
Organism source:                  A~ &\*tu4          Pr    &\"0\*IC          Artemia CHM number:        C.~t\31\
I                                                                                                  Drying information for weight determination:
Transfer bowl information:        pH= 1.1, s.u.                                Date I Time in oven:      11-Nl*IO      IllS I                  Average transfer volume:
Temperature =15.'1.!'C .
o.'~'N......O.
Initial oven temperature:     !Db. t.
Date I Time out of oven: 11-rb*IO Final oven temperature:      '-''(.
I         Daily feeding and renewal information:
Total drying time:        1.~*lo\bd~
Day        Date          Morning reeding                                  Test initiation,            Sample numbers used I                                                      Afternoon reeding renewal, or MHSW batch I
I I
I I
I IPage 50 of 105 SOP AT20 - Exhibit AT20.3, revision 04-01-09
 
Page 2 of6
              .;) Environmental Testing Solutions, Inc.
Species: Pimephales promelas                                    ,
Client: TVA I SeQuoyah Nuclear Plant, Outfall101. UV-treated                                        Date:     1\*0t*IO Survival and ,..._ *'- Data Day                          CONTROL                              11.3%                        22.6%
UV-treated A            B        c        D      E      F      G    H      I      J      K        L 0
ID ID                to        ID IO ID 10              10      ID 10          '0 10 1
                                                                  /0          IO        /0      I{)     IO    10    /0    10 IO        I~    10      10 2                                                       '
IO            IC        /Q        /Q    IC I(J      to    /0      IO      /Cj    10      10 3
lC IC It:>                      IO 10      lO    10    IO      lt>    10    it)       10 4
10          /0        10      10      10 tO        /0    to rc          I()     /Q      fQ 5
I0        IC          f()      10    10    lO    10    IQ      tO tD 10 ID 6
IO            I'D LO 10                IC 10        10 IO        lC      It>    10 10 7
10            r'()      /l)      IQ    *o      10    IC    to      /0    IO 10          tO' A= ~::.!e!~!~~j~ W.u.t
                                                                                ,_.'\0 \'-\!\1 ,,..,~ \?~~ ~~.-1\t \?a .'tl .\~tM ~,..~" I\~~ \'\.fb\ 1\1).~
                            -=rw.~                              ~?*"'
[
                                              'L.lO
*'I                          B ' Pan+          ~an:ae    weight j~                                              'f
                            ~:::;sn .n. ao C = Larvae weight (mg)
                            =B-A rzt.Cft ~--*-
1t\.f;t' lal15 Zf.'PC 21-=l-\ ~ lll,l~ 111-!H llh_ld I7J.'I~ ~'1.'7 ~~
1*"-1 ,,\.() &,7..\ '\.10 &.14\ '\,oR &.l.\. &.!tb &.2.1                    a.c.t ~*Sl    [1.\\
iu                          Weight per initial number oflarvae (mg)
  !~
                                                                                                                                                \o~
                                                                                                                                                      ~      "~
o*r# Cl~ 0~~
                                                                                        .c)      ~f:J                                ,~
                            = C I Initial Dllmber of
                                                                  ~
                                                                            ,...'-'0                      ~                          ~
                                                                                                                                              ~
().
lo~
larvae                                                                                                  b.            Cl.     (:)'
t>'       r:;,*      lo'       ()"
Avenge              *Percent
;~                          weight per initial reduction from        o.&-o"l                                 o. &Ia\       ~ z.&7..     o.~~            -o.~"t..
number of               control(%)
J!n'!elmg)
J!n'!elmg)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w =wounded.
:! 1:
Calculations and data reviewed: ICommmU:
u Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w =wounded.
I SOP A T20 -Exhibit A T20.3, revision 04-01-09 Page 51 of 105 ri" ' .ETS * . _: . : ,.,_,*,""-"""
Calculations and data reviewed:   ~
*'+ ;"*-,***':*.  
u IJ ICommmU:
*-* Environmental lUting Solutlans, Inc. Page 3 of6 I Species:
I SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 51 of 105
Pimephales promelqs I I I I I I I I I I I I I Client: TVA I Seauoyah Nuclear Plant. Outfall101.
 
UV-treated Date: t \-dl.*IO Survival and Growth Data Day 45.2% 72.6% 100% M N 0 p Q R s T u v w X 0 /D lb ID /Q /Q 'D ID /D /0 /0 10 /0 l I() lb 10 /0 /0 /() 10 I() 10 /0 /Q 10 2 to 10 IO lb I() 10 10 /() 10 IO 10 /b 3 ID I() IC> t() ID /0 I() tO fD I() /C lt) 4 /D It> /0 lO tO 10 IC 10 LC 10 'C 10 5 It> lC lO ID lt> II.) IQ lO /Q IC to /{) 6 /D (Q 10 LO IC /() IC IQ ID 10 I(J 10 7 ID to rc IC l() IO /() to IO IO 10 10 A= Pan welgbt ft! tV'' l4J.\"  
r1 ..ETS i"
\'i.\'\ Tray color Analyst:
Page 3 of6
Date: \CfM. .\tl 8 = Pan + Larvae weight (mg) '(" 2tfU 'Z.\'(=1 z.a.tt5 z;z.ctt; 1};t;'6 L'1.0l 1tt3 23::1-1 V(,'IJ, Analyst*
  ~--      : ,. _,* " -" "   *'+     ;"*-,***':*.
  *-*       ~ Environmental lUting Solutlans, Inc.
~ I                           Species: Pimephales promelqs Client: TVA I Seauoyah Nuclear Plant. Outfall101. UV-treated                                   Date:       t \-dl.*IO I                                  Day                               45.2%
Survival and Growth Data 72.6%                                   100%
M         N       0       p     Q         R     s           T         u         v       w           X I                                    0
                                                            /D       lb ID           /Q     /Q       'D ID           /D         /0         /0       10 /0 l
                                                                                                                                                        /0 I                                  2 10 to I()
10 lb IO 10
                                                                                    /b
                                                                                              /0 lb
                                                                                                      /0 I()
                                                                                                              /()
10 10 10 I()
                                                                                                                                    /()
10 10         IO 10
                                                                                                                                                                  /Q I                                  3 4
ID       I()     IC>       t()   ID       /0     I()       tO         fD       I()     /C         lt)
                                                          /D       It>   /0       lO     tO       10     IC       10           LC       10       'C         10 I                                  5 It>     lC       lO       ID     lt>       II.) IQ         lO         /Q       IC       to         /{)
6
                                                          /D       (Q                       IC       /()     IC         IQ         ID 10             I(J       10 I                                  7 ID     to 10 rc LO IC     l()     IO     /()       to         IO         IO       10         10 ft!
I                A= Pan welgbt (~
Tray color;;;e::,~\At Analyst:                            ~eM tV'' l4J.\"     ~.f) ~::\") \'t.\~ \"\.~ ~"\~ ~fb\ \'?~ \~.'\1 \'i.\'\
Date: \CfM. .\tl I                8 = Pan + Larvae weight (mg)
Analyst*      '("
Date: \l* \\
Date: \l* \\
* l D C "' Larvae weigbt (mg) "'8-A
* l D 2tfU    'Z.\'(=1 z.a.tt5 tz.\~    z;z.ctt; 1};t;'6 22-Cf~  L'1.0l ~1-2 1tt3 23::1-1 V(,'IJ, C "' Larvae weigbt (mg)
&.'\1 a.!M &o'LO &.'tO.
I                "'8-A S.'~1    '\.~    &.'\1    a.!M &o'LO      &.'tO.  \*~"'     '\.~1. a.'t\ l.I)C)        ~.1.'\     '\.cr'\
a.'t\ l.I)C)
I                Weight per initial number of larvae (mg)
'\.cr'\ Weight per initial number '"""'  
                                                          ~~        ~      ~  "      ~)...
'-'"'  
                                                                                            "'~      ~
,__. f:lt..t-of larvae (mg) " = C I Initial number of larvae K "' '" ,._,.., 0' o* o* o* o* o* o* t1 b' ()* I Average Percent weight per reduction 0.t'-1 -!>>.I. 't o. tlo"\ *'!t."Z.7.
                                                                                                      ~      {/:'~    K '-'"'    ~          ~'t>
: o. &&.2.
                                                                                                                                                      ,._,..,     f:lt..t-
initial from control number of (%) larvae (me:) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.
                  = C I Initial number of larvae                  ~                ~
* Calculations and data reviewed:
0'      o*       o*      o*      ~*      o* o*            o*          t1      b'        ()*      I ~*
J(__ I Comments:
I                 Average weight per initial Percent reduction from control      0 .t'-1          - !>>.I.'t      o. tlo"\        *'!t."Z.7.            o. &&.2.             -~.l7.
I I SOP A T20-Exhibit A T20.3, revision 04-0 l-09 Page 52 of 105 I II I I I I I I I &#xa3;ETS Page 4 of6 Envlronmentol Tostlng Solution.,
number of            (%)
Inc. Species:
I                larvae (me:)
Pimephales urome/as Client: TVA I Seguoyah Nuclear Plant, OutfalllOl.
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.
UV-treated Date: 1\:cn. *to Day per oflarvae (mg) 0 1 2 3 4 5 6 7 = C l Initial number of larvae /0 (0 ID (0 /0 'D 10 to /fl ID I() 10 (() Average weigbt per initial number or larvae reduction from control 0* &'\ \ (%) /0 10 (() 10 lQ IO 10 10 /C IO /Q Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm =unusually small, lg =unusually large, d&r = decanted and returned, w = wounded.
* I                                                                                                                            Calculations and data reviewed:           J(__
Calculations and data reviewed:
Comments:
L I SOP A T20 -Exhibit A T20.3, revision 04-01-09 Page 53 of 105  
I I
.... v., * * *
I Page 52 of 105 SOP AT20- Exhibit AT20.3, revision 04-0 l-09
* e 0 Environmental Testing Solutions, Inc. eo-a-(%)
 
...,._ A Coaln>l B c D E F 11.3% G H I J 12.6% K L M 45.2% N 0 p (j R 72,6% s T u v 100% w X y z lutake AA DB OpJfa!IIOJ:
Page 4 of6
Da*aett'a MSD val*e: PMSD: Ia take: Dauett'*
          &#xa3;ETS
MSD value: PMSD: ...........
          ~ Envlronmentol Tostlng Solution., Inc.
4 --10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 8.1035 11.4 0.0675 8.1 ,.... ........
Species: Pimephales urome/as Client: TVA I Seguoyah Nuclear Plant, OutfalllOl. UV-treated                    Date:      1\:cn. *to Day 0
10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 A*Pu ...... l("'l) 13.41 12.90 14.92 12.88 13.92 13.56 13.92 13.04 12.59 13.26 12.81 15.06 13.54 14.46 14.46 13.85 14.75 14.29 14.43 14.54 13.81 13.73 14.42 15.19 14.20 1325 13.22 13.74 MSD* PMSD* TVA I Sequoyah Nuclear Plant, OutfalllOl UV-treated November 02-09,2010 Pimephales promelas Cbronic Wbole Effluent Toxicity Test EPA-821-R-02-013, Metbod 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Not for C<>mpliance A>oessment.
1
Internal Labonltorv 6c .......  
                                                              /0 /0  10 2
* ..............  
(0     ID  (() 10 3
( ... Wdpi/S ........  
(0      /0  lQ  IO 4
....... ("'&) *A*B ...... atlarY*(-e) 21.98 8.57 0.857 20.50 7.60 0.760 23.13 8.21 0.821 21.98 9.10 0.910 22.71 8.79 0.879 22.64 9.08 0.908 22.18 8.26 0.826 21.34 8.30 0.830 20.87 8.28 0.828 21.94 8.68 0.868 22.32 9.51 0.951 22.37 7.31 0.731 21.91 8.37 0.837 23.47 9.01 0.901 23.43 8.97 0.897 22.19 8.34 0.834 22.95 8.20 0.820 22.58 8.29 0.829 22.97 8.54 0.854 24.06 9.52 0.952 22.72 8.91 0.891 21.73 8.00 0.800 23.71 9.29 0.929 24.26 9.07 0.907 22.99 8.79 0.879 22.01 8.76 0.876 21.97 8.75 0.875 23.09 9.35 0.935 Minimum SignifiCIIIll Difference Percent Minimum Significant Difference Meu ..... t/ Codlideat aharla.._
                                                      'D    10  10  10 5
& ................  
to      /fl /C 6
<M-..................
ID      I() IO 7
lonoe("'l)
10     (()  /Q I
........  
II I                        per oflarvae (mg)
.._)(%) 0.837 7.5 0.861 4.6 0.845 10.8 0.867 4.2 0.864 7.0 0.882 6.4 0.891 3.3 Weipt / .. ltlal .. *ber flllan*<-1) 0.857 0.760 0.821 0.910 0.879 0.908 0.826 0.830 0.828 0.868 0.951 0.731 0.837 0.901 0.897 0.834 0.820 0.829 0.854 0.952 0.891 0.800 0.929 0.907 0.879 0.876 0.875 0.935 Project aamber: .. 67119 Reveiwodby:
I              = C l Initial number of larvae Average weigbt per              reduction I              initial number or larvae from control
r' '=ll .... ..,. ..... -. ....... , ....... .. ...... ....... ,,_ (%) *-berfiiJano
(%)
.. .........
0* &'\ \
oc-...... --(%) ("'I) .........
I                  Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm =unusually small, lg =unusually large, d&r = decanted and returned, w = wounded.
100.0 8.837 7.5 Not applicable 100.0 11.861 4.6 -%.8 100.0 11.845 10.8 -11.9 100.0 11.867 4.2 -3.6 100.0 0.864 71J -3.2 100.0 11.882 6.4 -5.3 100.0 0.891 3.3 -6.5 -..... -PMSD is a measure of test precision.
Calculations and data reviewed: L I
The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole eftluent toxicity test Lower PMSD bound determined by USEPA (I Oth percentile)
I                                                                                                                                    I IPage 53 of 105 SOP AT20 -Exhibit AT20.3, revision 04-01-09
= 12%. Upper PMSD bound detennined by USEPA (90th percentile)=
 
Lower and uppe< PMSD bounds were determined from the lOth and 90th percentile, respeetively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200la; USEPA, 200lb). USEPA. 2001 a, 2001 b. Final Report: Jnterlaboratooy Variability Study of EPA Short-term Chronic and Acute Whole.Effiuent Toxicity Test Methods, Volumes I and 2-Appendix.
~
EPA-821 -B-01-004 and EPA-821-B-01
~
-005. US Environmental Protection
~                                                                                                              TVA I Sequoyah Nuclear Plant, OutfalllOl UV-treated
: Agency, OH.
~
:5; .ETS * * * @:> ,_ ,, .. ,*<' _*_, *c" C*.('*'c>-Environmental Testing Solutions, Inc. Start Date: 111212010 End Date: 11/9/2010 Sample Date: Comments:
~
UV-Treated Cone-% I 2 D-Control 0.8570 0.7600 I 1.3 0.8790 0.9080 22.6 0.8280 0.8680 45.2 0.8370 0.9010 72.6 0.8200 0.8290 100 0.8910 0.8000 Intake 0.8790 0.8760 Cone-% Mean N-Mean D-Control 0.8370 1.0000 11.3 0.8608 1.0284 22.6 0.8445 1.0090 45.2 0.8673 1.0361 72.6 0.8638 1.0320 100 0.8818 1.0535 Intake 0.8913 1.0648 Auxili!!!l:
November 02-09,2010 v.,
Tests TV A I Sequoyah Nuclear Plant, OutfalllOl UV-treated November 02-09,2010 Statistical Analyses Larval Fish Growth and Survival Test-7 D!!I Growth TestiD: PpFRCR SampleiD:
Pimephales promelas Cbronic Wbole Effluent Toxicity Test EPA-821-R-02-013, Metbod 1000.0 e                                                                                                                                  Quality Control Verification of Data Entry, Calculations, and Statistical Analyses 0        Environmental Testing Solutions, Inc.                                                                                                                                                                                     Project aamber:
TV A I Sequoyah Nuclear Plant, Outfall I 01 LabiD: ETS-Envir.
r' 11~
Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol:
                                                                                                                                                                                                                                                                      ..                            67119 eo-a-(%)           ...,._
FWCHR-EPA-821-R-02-013 Test Species:
                                      ........... 4    ,.... ........ ar~an-. A*Pu ......l("'l)  ....... ~.an*
PP-Pimephales promelas 3 4 0.8210 0.9100 0.8260 0.8300 0.9510 0.7310 0.8970 0.8340 0.8540 0.9520 0.9290 0.9070 0.8750 0.9350 Transform:
                                                                                                    ....... ("'&)
Untransformed
                                                                                                                        *A*B
!-Tailed Isotonic Mean Min Max CV% N t-Stat Critical MSD Mean 0.8370 0.7600 0.9100 7.529 4 0.8592 0.8608 0.8260 0.9080 4.608 4 -0.553 2.410 0.1035 0.8592 0.8445 0.7310 0.9510 10.820 4 -0.175 2.410 0.1035 0.8592 0.8673 0.8340 0.9010 4.234 4 -0.705 2.410 0.1035 0.8592 0.8638 0.8200 0.9520 7.012 4 -0.623 2.410 0.1035 0.8592 0.8818 0.8000 0.9290 6.428 4 -1.042 2.410 0.1035 0.8592 0.8913 0.8750 0.9350 3.278 4 Statistic Critical Skew Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98219049 0.884 -0.040311724 Bartlett's Test indicates Test 0.052 Dunnett's Test Treatments vs D-Control Point !COS IC10 ICI5 IC20 IC25 IC40 IC50 % >iOO >100 >100 >100 >100 >100 >100 NOEC LOEC 100 >100 SD 95% CL(Exp) 2.94596696 15.0862722 ChV TU MSDu MSDp MSB MSE I 0.10347888 0.12363068 0.00104427 0.00368722 Linear Interpolation (200 Resamples)
(
Skew F-Prob 0.91621083 N-Mean 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Kurt -0.1382183 df 5, 18 sqn101_110210data 
Not for C<>mpliance A>oessment. Internal Labonltorv 6c Wdpi/S........
: v. Q\ lo...o TV A I Sequoyah Nuclear Plant, OutfalllOl
                                                                                                                                    ...... atlarY*(-e)
-Intake UV-treated
Meu .....t/
.ETS * *
lonoe("'l)
* e .: .,: .. , , . ."..'* ':* " .. Environmental Testing Solutions, Inc. Start Date: 11/2/2010 End Date: 11/9/2010 Sample Date: Conunents:
Codlideat aharla.._
UV-treated Cone-% I 2 D-Control 0.8570 0.7600 11.3 0.8790 0.9080 22.6 0.8280 0.8680 45.2 0.8370 0.9010 72.6 0.8200 0.8290 100 0.8910 0.8000 Intake 0.8790 0.8760 Cone-% Mean N-Mean D-Control 0.8370 1.0000 11.3 0.8608 1.0284 22.6 0.8445 1.0090 45.2 0.8673 1.0361 72.6 0.8638 1.0320 100 0.8818 1.0535 Intake 0.8913 1.0648 Auxili!!!1 Tests November 02-09, 2010 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth TestiD: PpFRCR SampleiD:
                                                                                                                                                                              <M- ..................
LabiD: ETS-Envir.
                                                                                                                                                                                .........._)(%)
Testing Sol. Sample Type: Protocol:
Weipt / ..ltlal ..*ber flllan*<-1)
FWCHR-EPA-821-R-02-013 Test Species:
(%)
3 4 0.8210 0.9100 0.8260 0.8300 0.9510 0.7310 0.8970 0.8340 0.8540 0.9520 0.9290 0.9070 0.8750 0.9350 Transform:
Reveiwodby:
Untransformed Mean Min Max CV% N t-Stat 0.8370 0.7600 0.9100 7.529 4 0.8608 0.8260 0.9080 4.608 4 0.8445 0.7310 0.9510 10.820 4 0.8673 0.8340 0.9010 4.234 4 0.8638 0.8200 0.9520 7.012 4 0.8818 0.8000 0.9290 6.428 4 0.8913 0.8750 0.9350 3.278 4 -1.562 Statistic Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93294114 F-Test indicates
                                                                                                                                                                                                                                            *-berfiiJano..
!:jUal variances
("'I)
!2 = 0.24) 4.65254307 Hn!2thesis Test 0.05) MSDu MSDp Homoscedastic t Test indicates no significant differences 0.06748823 0.0806311 Treatments vs D-Control TV A I Sequoyah Nuclear Plant, Intake DMR-Discharge Monitoring Report PP-Pimephales promelas 1-Tailed Critical MSD 1.943 0.0675 Critical Skew Kurt 0.749 0.02117318 0.51937165 47.4672279 MSB MSE F-Prob df 0.00588612 0.00241246 0.16931061 1, 6 sqnl 01_1102 /Odata 
                                                                                                                                                                                                                                                                '=ll
: v. "'-l ..... TVA I Sequoyah Nuclear Plant, Outfall 101 -UV-treated November 02-09, 2010 . ETS * * *
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. .. .. >*':. Environmental Testing Solutions, Inc. Coneentration Control 11.3% 22.6% 45.2*/a 72.6% 100% 100% Intake lkalinity (mgtL LaLU:J) b4 Hardaess (mg/L CaCO:J) 72 --. --------.... , ... Pimephales promelas Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number: 6709 I I I I I I I I I I I I I I I I I I I .ETS *
                                                                                                                                                                                                                                                              ......... oc-......
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                                                                                                                                                                                                                                                                    ~.,.....,
-. *'. . .. **:'<. ' . . . . ..J Envlronment*l Testing Solutions, Inc. Page 5 of6 Species:
                                                                                                                                                                                                                                                                                        --(%)
Pimepha/es prome/as Date: __,li..Ll,..,.<n.._*...,IDe--
A              10                    10              13.41              21.98              8.57            0.857                                                            0.857 Coaln>l B              10                    10              12.90              20.50              7.60            0.760 0.837                  7.5 0.760 100.0            8.837                7.5            Not applicable c              10                    10              14.92              23.13              8.21            0.821                                                            0.821 D              10                    10              12.88              21.98              9.10            0.910                                                            0.910 E              10                    10              13.92              22.71              8.79             0.879                                                             0.879 F              10                    10              13.56              22.64              9.08            0.908                                                            0.908 11.3%                                                                                                                                              0.861                  4.6                                    100.0            11.861                4.6                  -%.8 G              10                    10              13.92              22.18              8.26            0.826                                                            0.826 H              10                    10              13.04              21.34              8.30            0.830                                                            0.830 I              10                    10              12.59              20.87              8.28            0.828                                                            0.828 J              10                    10              13.26              21.94              8.68            0.868 0.845 0.868 12.6%                                                                                                                                                                    10.8                                    100.0            11.845              10.8                  -11.9 K              10                    10              12.81              22.32              9.51            0.951                                                            0.951 L              10                    10              15.06              22.37              7.31            0.731                                                            0.731 M              10                    10              13.54              21.91              8.37            0.837                                                            0.837 N              10                    10              14.46              23.47              9.01            0.901                                                            0.901 45.2%                                                                                                                                              0.867                  4.2                                    100.0           11.867               4.2                 -3.6 0             10                    10              14.46              23.43              8.97            0.897                                                            0.897 p              10                    10              13.85              22.19              8.34            0.834                                                            0.834 (j            10                    10              14.75              22.95              8.20            0.820                                                            0.820 R              10                    10              14.29              22.58              8.29            0.829                                                            0.829 72,6%                                                                                                                                              0.864                  7.0                                   100.0           0.864                71J                  -3.2 s              10                    10              14.43              22.97              8.54            0.854                                                            0.854 T              10                    10              14.54              24.06              9.52            0.952                                                             0.952 u              10                    10              13.81              22.72              8.91            0.891                                                            0.891 100%
__ _ Client: TV A I Seauoyah Nuclear Plant. OutfalllOl.
v              10                    10              13.73              21.73              8.00            0.800 0.882                  6.4 0.800 100.0            11.882                6.4                  -5.3 w              10                    10              14.42              23.71              9.29            0.929                                                            0.929 X              10                    10              15.19              24.26              9.07            0.907                                                            0.907 y              10                    10              14.20              22.99              8.79            0.879                                                            0.879 IOU"~ lutake z              10                    10              1325                22.01              8.76            0.876 0.891                  3.3 0.876 100.0            0.891                3.3                  -6.5 AA              10                    10              13.22              21.97              8.75            0.875                                                            0.875 DB              10                    10              13.74              23.09              9.35            0.935                                                              0.935              -     ..... -
UV-treated CONTROL UV-treated 11.3% 22.6% 4S.Z% 72.6% 100% 100% Intake SOP AT20-Exhibit AT20.3, revision 04-01-09 Page 58 of 105 
OpJfa!IIOJ:                                                              MSD*              Minimum SignifiCIIIll Difference Da*aett'a MSD val*e:            8.1035                                    PMSD*            Percent Minimum Significant Difference PMSD:                          11.4                                                        PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole eftluent toxicity test Ia take:
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Dauett'* MSD value:            0.0675                                                      Lower PMSD bound determined by USEPA (I Oth percentile) = 12%.
....... " ; '.--..
PMSD:                          8.1                                                        Upper PMSD bound detennined by USEPA (90th percentile)= 30'~.
*.* Environmental Tostlng Solutions, Inc. 11.3% 22.6% 45.2% 72.6% 100% 100&deg;/o Intake I !Page 59of105 Page 6 of6 C:, I .= 1. & \
Lower and uppe< PMSD bounds were determined from the lOth and 90th percentile, respeetively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200la; USEPA, 200lb).
3ot* 1:)0:., SOP A T20-Exhibit A T20.3, revision 04-0 l-09 ll n II l -I ] l .ETS * * * 
USEPA. 2001 a, 2001 b. Final Report: Jnterlaboratooy Variability Study of EPA Short-term Chronic and Acute Whole.Effiuent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821 -B-01-004 and EPA-821-B-01 -005. US Environmental Protection Agency, Cincinnat~ OH.
.. :c-, -*-'-* -*.,.,,., Environmental Testing Salution-.lnc.
 
Analyst I Date analyzed u* . I 0 L b I d, d. a oratory contro stan ar. : Reference standard number lJ\J SS':l:_(uCQ Note: All samples were analyzed in excess Page 60 of 105 Total Residual Chlorine Page PageL ott (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L -I Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent:
~
IWj{g \ lo Acid reagent: L \ '2... ' True value (TV) value (MV) %RS=MV/TVx 100 (mg/L) (mg/L) (acceptable range = 90 to 110%) o.so U.')l\ lO 2.. 'J-"t. Sample characteristics recommended holding time (lS minutes) unless otherwise noted. True value (TV) (mg!L) 0.50 % RS = MV I TV x 100 (acceptable range= 90 to 110%) Reviewed by Date reviewed
~                                                              TVA I Sequoyah Nuclear Plant, OutfalllOl
._ __
~
..... 1;,.:0:;.,_---J SOP C8 -Exhibit C8.l, revision  
      .ETS UV-treated
'\. \
~
I iETS Environmental Testing Inc. Analyst I Date analyzed
~
: n. I I 0 Calibration:
:5; ***                                                                            November 02-09,2010 Statistical Analyses
Laborato control stantlard:
    @:> ,_ ,,..,*<' _*_,    *c"      C*.('*'c>-
Reference standard Laborato control standard:
    ~    Environmental Testing Solutions, Inc.
Total Residual Chlorine Page Page_(_ of --2-(Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter True value (TV) (mg!L) 0.50 Iodide reagent:
Larval Fish Growth and Survival Test-7 D!!I Growth Start Date:          111212010                      TestiD:      PpFRCR                                    SampleiD:                TVA I Sequoyah Nuclear Plant, Outfall I 01 End Date:            11/9/2010                      LabiD:      ETS-Envir. Testing Sol.                   Sample Type:            DMR-Discharge Monitoring Report Sample Date:                                      Protocol:   FWCHR-EPA-821-R-02-013                    Test Species:            PP-Pimephales promelas Comments: UV-Treated Cone-%                I                2            3          4 D-Control            0.8570            0.7600        0.8210      0.9100 I 1.3          0.8790            0.9080        0.8260      0.8300 22.6          0.8280            0.8680        0.9510      0.7310 45.2           0.8370           0.9010       0.8970      0.8340 72.6          0.8200            0.8290       0.8540      0.9520 100           0.8910           0.8000       0.9290      0.9070 Intake           0.8790           0.8760       0.8750      0.9350 Transform: Untransformed                                      !-Tailed                              Isotonic Cone-%               Mean         N-Mean           Mean        Min          Max          CV%              N        t-Stat      Critical      MSD              Mean            N-Mean D-Control            0.8370             1.0000       0.8370      0.7600        0.9100          7.529      4                                                            0.8592      1.0000 11.3          0.8608            1.0284      0.8608      0.8260        0.9080          4.608      4          -0.553        2.410    0.1035                  0.8592      1.0000 22.6          0.8445            1.0090        0.8445      0.7310        0.9510        10.820        4          -0.175        2.410    0.1035                  0.8592      1.0000 45.2          0.8673            1.0361      0.8673      0.8340        0.9010          4.234      4          -0.705        2.410    0.1035                  0.8592      1.0000 72.6          0.8638            1.0320        0.8638      0.8200        0.9520           7.012      4          -0.623        2.410    0.1035                  0.8592      1.0000 100          0.8818            1.0535        0.8818      0.8000        0.9290          6.428      4           -1.042        2.410    0.1035                  0.8592      1.0000 Intake          0.8913            1.0648      0.8913      0.8750        0.9350          3.278      4 Auxili!!!l: Tests                                                                                            Statistic                Critical                      Skew                Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01)                                                0.98219049                  0.884                    -0.040311724        -0.1382183 Bartlett's Test indicates ~ual variances~= 0.71~                                                            2.94596696              15.0862722 H~thesis Test ~1-tail, 0.052                          NOEC        LOEC          ChV            TU            MSDu      MSDp          MSB          MSE              F-Prob              df Dunnett's Test                                          100        >100                            I        0.10347888 0.12363068 0.00104427 0.00368722            0.91621083            5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples)
I \ Uo Acid reagent:  
Point                    %                SD              95% CL(Exp)            Skew
\ t\\ Reference standard True value (TV) Measured value (MV) (mg/L) number (mg!L) IN ,to&' 0.50 Page 61 of 105 o. Reviewed by Date reviewed
      !COS                    >iOO IC10                      >100 ICI5                       >100 IC20                      >100 IC25                      >100 IC40                      >100 IC50                      >100 sqn101_110210data
*
 
...... SOP C8 -Exhibit CS.I, revision 09-01-09 I I I I I I I I I. t t *-ETS ** * * * . ' ' " ,_,, 0 Environmental Testing Solutlanr,lnc.
~
Date: I n.fl 10 Laborato control standard:
~                                                      TVA I Sequoyah Nuclear Plant, OutfalllOl -Intake v.
Reference standard Page 62 of 105 Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Page 65.
Q\
Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent:
UV-treated
I S: Acid reagent: > 1. 0 m!VL, the calibration range must be True value (TV) (mg!L) 0.50 Sample characteristics True value (TV) (mg!L) 0.50 Measured value (MV) * (mg!L) 0* q unless otherwise noted. Measured value (MV) (mg!L) o. Reviewed by I d{ Date reviewed SOP C8 -Exhibit C8 .1, revision 09-0 1-09 I I I -I I I I I I I I I I !ETS Environmental Testing Solutions, Inc. Analyst I Date analyzed
~
\: 0 (&, * \ 0 L b I tJ d. a oratory contro stan ar, : Reference standard number
lo...o November 02-09, 2010
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~
Reference standard number Total Residual Chlorine Page 1?1 (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter ' True value (TV) value (MV) (mg/L) (mg/L) 0.50 0. Z:St\) Sample chancteristics Sample characteristics True value (TV) Measured value (MV) (mg/L) (mg/L)
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Iodide reagent:
      ~
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1:-h.) f.. l'Z,.. of the samples.  
Environmental Testing Solutions, Inc.
% RS=MV /TVx 100 (acceptable range= 90 to 110%) l 01... .0 .,lo % RS = MV I TV x 100 (acceptable range= 90 to 110%) *tt\C Reviewed by I Date reviewed SOP C8 -Exhibit C8.1, revision 09-01-09 I I ' ,. I I iETS Environmental Testing Solutions, Inc. Analyst I M !' Date analyzed  
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11/2/2010                                 TestiD:      PpFRCR                                  SampleiD:                  TVA I Sequoyah Nuclear Plant, Intake End Date:             11/9/2010                       LabiD:      ETS-Envir. Testing Sol.                Sample Type:              DMR-Discharge Monitoring Report Sample Date:                                           Protocol:    FWCHR-EPA-821-R-02-013                  Test Species:              PP-Pimephales promelas Conunents: UV-treated Cone-%                   I                 2             3          4 D-Control               0.8570           0.7600       0.8210      0.9100 11.3             0.8790           0.9080       0.8260      0.8300 22.6             0.8280           0.8680       0.9510      0.7310 45.2             0.8370           0.9010       0.8970      0.8340 72.6             0.8200           0.8290       0.8540      0.9520 100             0.8910           0.8000       0.9290      0.9070 Intake               0.8790           0.8760       0.8750      0.9350 Transform: Untransformed                                      1-Tailed Cone-%                 Mean         N-Mean             Mean        Min            Max        CV%            N          t-Stat      Critical    MSD D-Control               0.8370           1.0000       0.8370      0.7600        0.9100      7.529        4 11.3              0.8608            1.0284      0.8608      0.8260        0.9080      4.608        4 22.6             0.8445           1.0090       0.8445      0.7310        0.9510      10.820        4 45.2             0.8673           1.0361       0.8673      0.8340        0.9010      4.234        4 72.6              0.8638            1.0320       0.8638      0.8200        0.9520      7.012        4 100             0.8818           1.0535       0.8818      0.8000        0.9290      6.428        4 Intake               0.8913           1.0648       0.8913      0.8750        0.9350      3.278        4            -1.562        1.943    0.0675 Auxili!!!1 Tests                                                                                               Statistic                  Critical                  Skew          Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01)                                              0.93294114                    0.749                0.02117318 0.51937165 F-Test indicates !:jUal variances !2 = 0.24)                                                              4.65254307                47.4672279 Hn!2thesis Test ~1-tail, 0.05)                                                                                MSDu        MSDp          MSB        MSE        F-Prob          df Homoscedastic t Test indicates no significant differences                                                  0.06748823  0.0806311    0.00588612 0.00241246 0.16931061          1, 6 Treatments vs D-Control sqnl 01_1102 /Odata
\ l* 0 . t Q L b t ltd.rd a ora ory contro s an a : Reference standard number 1:.\-...)\\
 
Lbo a I ratory contro stan a : Reference standard number Page 64 of 105 Total Residual Chlorine Page foO Page ___1e_ of __1_ (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent:
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I ::::=---:......
~
I Acid reagent: of the samples.
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' True value (TV) Measured value (MV) % RS = MV I TV x 100 (mg!L) (mg/L) (acceptable range= 90 to llO%) 0.50 0.4-t':>t{
"'-l November 02-09, 2010
qt,S"L Sample characteristics True value (TV) Measured value (MV) % RS = MV I TV x 100 (mg!L) (mg!L) (acceptable range= 90 to 110%) u ... v TVI1 Reviewed by I ::If Date reviewed
~
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      ~
SOP C8 -Exhibit C8.1, revision 09-01-09 I I I I I .ETS * *
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        ~~,:-                      .
Reference standard Page __ {d_.
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Total Residual Chlorine (Orion Method, Orion 97-70) Matrix: Water, RL = 0.10 mgiL Meter: Accumet Model AR25 pH/Ion Meter True value (TV) (mg/L) 0.50 Sample characteristics Measured value (MV) (mg/L) 0. 1 Iodide reagent:
Environmental Testing Solutions, Inc.
                                                    !:~- >*':.
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Pimephales promelas Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number: 6709 Coneentration Control 11.3%
22.6%
45.2*/a 72.6%
100%
100% Intake                      lkalinity (mgtL LaLU:J)                          b4 Hardaess (mg/L CaCO:J)                              72
 
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Page 5 of6 I                        Species: Pimepha/es prome/as Client: TVA I Seauoyah Nuclear Plant. OutfalllOl. UV-treated Date: __,li..Ll,..,.<n.._*...,IDe--_ __
I I
I                        CONTROL UV-treated I
I                          11.3%
I                          22.6%
I I                          4S.Z%
I                          72.6%
I I                          100%
I I
I                          100%
Intake I
IPage 58 of 105 SOP AT20- Exhibit AT20.3, revision 04-01-09
 
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  !Page 59of105 SOP AT20- Exhibit AT20.3, revision 04-0 l-09
 
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l                                                                        Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L n
II Date analyzed Analyst    Iu*   ~
                                                        .I 0  -I Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent:
Acid reagent:
IWj{g L \'2...
                                                                                                                                                              \lo L aboratory controI stand,ar.d.:                                        '
Reference standard                        True value (TV)              Meas~red  value (MV)              %RS=MV/TVx 100 number                          (mg/L)                        (mg/L)                  (acceptable range = 90 to 110%)
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Sample characteristics lI
  ]
Note: All samples were analyzed in excess              recommended holding time (lS minutes) unless otherwise noted.
True value (TV)                                                  % RS = MV I TV x 100 (mg!L)                                                (acceptable range= 90 to 110%)
0.50 Reviewed by r--:';%1~r,....:-:--~
Date reviewed .___...;.lFC_~
                                                                                                                                                        .....~-~1;,.:0:;.,_---J
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lPage 60 of105                                                                                                        SOP C8 - Exhibit C8.l, revision          09-~
                                                                                                                                                                                '\.
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Page       SJ-Page_(_ of                 -            iETS
            ~  Environmental Testing Sol~ Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Analyst Date analyzed In. ~    I I0 Meter: Accumet Model AR25 pH/Ion Meter I\
Iodide reagent: N~ Uo Acid reagent: \ W~ ~        t\\
Calibration:
Laborato        control stantlard:
Reference standard                  True value (TV)
(mg!L) 0.50 Laborato        control standard:
Reference standard                  True value (TV)        Measured value (MV) number                          (mg!L)                     (mg/L)
IN          ,to&'                      0.50            o.
Reviewed by ~-"i:J~,..,...~-~
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IPage 61 of 105 SOP C8 - Exhibit CS.I, revision 09-01-09
 
Page  65.
Page~o{7
              .*-ETS 0
Environmental Testing Solutlanr,lnc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pH/Ion Meter IS:
Date: In.fl 10                                                                                        Iodide reagent:
Acid reagent:               ~
                                                                                                                                                                ~-
                                                      > 1. 0 m!VL, the calibration range must be I                                                    True value (TV)
(mg!L)
Measured value (MV)
                                                                                          *    (mg!L) 0.50                       0*      q I
Sample characteristics I
I I
I I
I unless otherwise noted.
Laborato        control standard:
Reference standard                  True value (TV)                Measured value (MV)
(mg!L)                              (mg!L)
I.                                                         0.50                      o.
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t                                                                                                                           Reviewed by Date reviewed t Page 62 of105 SOP C8 - Exhibit C8 .1, revision 09-0 1-09
 
Page  1?1 Page_\_of~
    !ETS
    ~  Environmental Testing Solutions, Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L I
Analyst ~\\{
Date analyzed \ : 0 (&, * \ 0 Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent:
Acid reagent:
                                                                                                                    '""!- "-l ~ ~\ (,
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I                                                                                                              of the samples.
L aboratory controI stantJar,d.:                              '
I      Reference standard number True value (TV)
(mg/L)
Mea~ilred value (MV)
(mg/L)
                                                                                                    % RS=MV /TVx 100 (acceptable range= 90 to 110%)
  "!.-~ <::. (_, 1- (,e.,                        0.50                  0. Z:St\)                    l 01... .0    .,lo I
Sample chancteristics
-I Duplicate I
Sample characteristics I
I I
I I
I   Laboratory control standard:
Reference standard                  True value (TV)          Measured value (MV)            % RS  =MV I TV x 100 I               number                        (mg/L) n_~n (mg/L)              (acceptable range= 90 to 110%)
                                                                                                                            *tt\C I                                                                                                    Reviewed by Date reviewed I~==1~\~-0~'-=*:l:o=:::~
                                                                                                                            ~
SOP C8 -Exhibit C8.1, revision 09-01-09
 
Page      foO Page ___1e_ of __1_
iETS
              ~ Environmental Testing Solutions, Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Date analyzed \ l* 0 I M!' t        . Q Iodide reagent:
Acid reagent:
I::::=---:......    ~
I of the samples.
Laboratory controltd.rd      s an a :                            '
Reference standard                      True value (TV)           Measured value (MV)     % RS = MV I TV x 100 number                                (mg!L)                       (mg/L)       (acceptable range= 90 to llO%)
1:.\-...)\\ 1(o~                                  0.50                   0.4-t':>t{                qt,S"L Sample characteristics I                a ratory controI stan a :
Lbo Reference standard                      True value (TV)           Measured value (MV)     % RS = MV I TV x 100 I
number                                (mg!L) u ...v (mg!L)        (acceptable range= 90 to 110%)
TVI1 I      ::If I                                                                                                              Reviewed by Date reviewed ._1-_-_,-,:,.:~:...
                                                                                                                                            ~i-:-_..-,:o:::~
I  Page 64 of 105 SOP C8 - Exhibit C8.1, revision 09-01-09
 
Page __      {d_.
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                .ETS e        -"'* *- *'  . . . .:]'.,;, ,_.
Envlronmentol Testing Solutions, Inc.*
Total Residual Chlorine (Orion ~lectrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mgiL Analyst ~ I h\&#xa3; Date analyzed \~ 0~- l D Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent: ~:......__.
Acid reagent: _
__::::'"""'=:::-----::--;
__::::'"""'=:::-----::--;
Acid reagent:
                                                                                                                                        <:::::::::::   \\\.f of the samples.
_ <:::::::::::  
Laborato          control standard:
\\\.f of the samples.  
Reference standard                    True value (TV)        Measured value (MV)      % RS=MV /TVx 100 (mg/L)                      (mg/L)      (acceptable range= 90 to 110%)
% RS=MV /TVx 100 (acceptable range= 90 to 110%) Note: All samples were analyzed in excess of EPA recommended Lbo I ntJ, rd. a ratory contro sta a : Reference standard number "!. 't\.\ \-
0.50                  0.        1 Sample characteristics I
I I I , 1 Page 65 of lOS True value (TV) Measured value (MV) (mg/L) (mg!L) 0.50 0 . t\--s'i % RS=MV /TV x 100 (acceptable range= 90 to 110%) '{ \. *t Reviewed by ,,_---"&rJ!-.  
I I
-----1 Date reviewed
I I
_ ___. SOP C8 -Exhibit C8.1, revision 09-01-09 Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Titrate samples to pH= 4.5 S.U. Titrant normality and multiJJiler determination:  
Note: All samples were analyzed in excess of EPA recommended a ratory controI stantJ,ard.:
.*-..,\.*  
Lbo Reference standard                   True value (TV)         Measured value (MV)     % RS=MV /TV x 100 number                        (mg/L)                     (mg!L)       (acceptable range= 90 to 110%)
.. """'"" ' pH of Normality Deionized Titrant check water reference standard  
                  "!. 't\.\ \- ~-'J~B                          0.50                 0 . t\--s'i                   '{ \. ~ *t Reviewed by ,,_---"&rJ!-.- - - - - 1 Date reviewed L..___.j~j.*o,ul.L.*..;.":=:V_ ___.
=4.SS.U.
I II      ,                                                                                            SOP C8 -Exhibit C8.1, revision 09-01-09 1  Page 65 of lOS
number number '-l.z L b I d d a oratory contro stan ar, : Reference standard True value number (TV) (mg CaCO,VL) 100 Sample ID J Duplicate (B) atrtx spt e recovery:
 
Reference standard Spike value number (SV) (ma CaCOJIL) ffi) Begin ml 00 Sample volume (ml) 100 Sample volume Sample volume (ml) I()D Sample alkalinity (B) (mg CaCOJIL) Sa le measurements:
Alkalinity (SM 2320 B)
66 of105 End ml II.IP Begin ml Begin ml Normality (N) ofH1S04 pH Factor orMitlii()iier*
Matrix: Water, RL = 1.0 mg CaC03/L Titrate samples to pH= 4.5 S.U.
Total = (5 ml Na1C03 x 0.05)/E = (Nx 50000)/100 nil sample ml =0.25/E =NxSOO (E) (acceptable range= 0.0180
Titrant normality and multiJJiler determination:                                                                                 .*-..,\.* .. """'"" ~
* 0.0220) 1/.tp 6.n21co /6,(3 I' Alkalinity (MV) o/o RS= MV /TV x 100 End Total Multiplier (mg CaC03/L)  
pH of                     Normality                                     Normality (N) ofH1S0 4                pH Factor orMitlii()iier*
(acceptable range ml ml =90 to 110%)
Deionized     Titrant         check     Begin      End    Total        = (5 ml Na1C03 x 0.05)/E            = (Nx 50000)/100 nil sample water      reference      standard     ml        ml      ml                  =0.25/E                                  =NxSOO
q,_3
      =4.SS.U.       number         number                         (E)    (acceptable range= 0.0180
/00 (6b,D'/ Alkalinity o/oRPD = Total Multiplier (mg CaCO,VL)
* 0.0220)
{(S *D) /[(S+D)/2)}
        '-l.z     )Nt~zti        l,l(SSOl~    00        II.IP 1/.tp                6.n21co                                  /6,(3 Laboratory controI standar,d:
x 100 (acceptable range= :1: 10%) Spike alkalinity (A) End Total Multiplier (mg CaCDJIL) ml ml 3:2{) e..o 1o.e e(p Measured spike value (MV) 0/o R=MV I SV x 100 MV=A-B (acceptable range (mr CaCOJIL)  
Reference standard       True value     Sample                  I'                            Alkalinity (MV)        o/o RS= MV /TV x 100 number                (TV)        volume      Begin    End      Total    Multiplier      (mg CaC03/L)               (acceptable range (mg CaCO,VL)       (ml)        ml      ml        ml                                                  =90 to 110%)
=75 to 125%) 5Z 16'-l.o 1 Date reviewed:
I~'SSPRil              100          100 IL*~ ~0.1          q,_3      ln.~                /00                     (6b,D'/
I /b*at .. 1o SOP C6-Exhibit C6. I, revision 09-0 l-09 I I I I I I I I I I I I
Sample                                                   Alkalinity                  o/oRPD =
. J" \. &#xa3;""".,* 0 , .. Jl L ) EnvlronrnontaiTesdng Inc. Analyst I Date analyzed Titrant *"* *and .........  
Sample ID          volume                       Total    Multiplier    (mg CaCO,VL)           {(S *D) /[(S+D)/2)} x 100 (acceptable range= :1: 10%)
.. _,_ Normality 0 Titrant check Begin standard ml =4.5S.U.  
J        Duplicate (B) atrtx spt e recovery:
'JC..UUUit::J" number "' T.  
Reference standard      Spike value    Sample                                                              Spike alkalinity (A) number                (SV)       volume      Begin    End      Total   Multiplier                      (mg CaCDJIL)
.1, Reference standard Sample number volume (mgi' .. co .. (ml) 100 Page q g Page 2 of?-Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC031L End ml Begin ml Titrate samples to pH=4.5 S.U. Time initiated Time completed
(ma CaCOJIL)       (ml)         ml      ml      ml IN~eao                    ffi)        I()D    ~\1.0  3:2{)      e..o        1o.e                      e(p 0
[ ____ ....,;;..
Sample alkalinity (B)         Measured spike value (MV)              /o R=MV I SV x 100 (mg CaCOJIL)                       MV=A-B                            (acceptable range (mr CaCOJIL)                        =75 to 125%)
__ ..... Normality (N) of H2S04 pH Factor or Multiplier Total = (5 ml Na2C03 x 0.05)/E = (N x 50000)/ 100 ml sample ml =0.25/E =Nx500 (E)
                                                  ~                            5Z                                  16'-l.o 1 Sa    le measurements:
* range-0.0180-0.0220) / Alkalinity (MV) % RS-MV /TV x 100 End Total Multiplier (mg CaCO,VL)
Date reviewed:      I /b*at .. o          1 SOP C6- Exhibit C6. I, revision 09-0 l-09 66 of105
(acceptable range ml ml = 90 to 110%) ll11nll,.,.,,..
 
sample ... "' Sample Alkalinity  
qg
%RPD= Sample Sample ID volume Total Multiplier (mg CaCOJt'L)
            =~.*.
{(S-D) /[(S+D)/2]}
Page 0 , ..
x 100 number (ml) ml "' s Duplicate (B) ""' D Mall'ix spike '"'""""''J*  
Jl J  \. "
" Reference standard Spike value Sample . Spike alkalinity (A) number (SV) volume Begin End Total (mg CaCOJIL)
L
(mg c .. co.JJ ,\ (ml) ml ml ml '"'-"' "' Sample alkalinity (B) Measured spike value (MV) *A, sv X 100 (mg CaCO,VL)
                                        &#xa3;""".,*                                                                                              Page        2    of?-
MV=A-B  
            ) EnvlronrnontaiTesdng  Solution~ Inc.
.. (mg CaCn./1.\  
Alkalinity (SM 2320 B)
"'!," s ample measurements:
Analyst        I       ~
Sample volume Begin End Total Alkalinity Sample number Saii!Pie ID (mil ml ml ml Multiplier
Matrix: Water, RL = 1.0 mg CaC0 31L Time initiated fr---'~~----t Date analyzed            :=:Jf=-=~=\.:jO====                            Titrate samples to                      Time completed [              ~ ....,;;.. .....
_ime CaCO,VL) lb.toz.t.o1.-
                                  *"* *and ......... ..
i'"'l'A  
pH=4.5 S.U.
: z. IOU 4{)0, J0-8. '-({!;
Titrant
I .3 I 'lo.tf  
          ~of                                      Normality                                          Normality (N) of H 2S04              pH Factor or Multiplier Titrant                check        Begin        End    Total        = (5 ml Na2C03 x 0.05)/E            = (N x 50000)/ 100 ml sample 0      Kefe~~nce                standard          ml          ml      ml                 =0.25/E                                =Nx500
'-/.-; ( Lfti -------rv* ......... ......--:.  
            =4.5S.U.           'JC..UUUit::J"        number                              (E)              *range- 0.0180- 0.0220)
-Reviewed by: Date reviewed:
I T.
I I j Page67ofl05 SOP C6-Exhibit C6.1, revision 09-01-09 I I I I I E *ers * ; I
number
*_, .* , )L_ . ,.-.J )
                            *~~o.L~
Analyst I
Reference standard      "'            **~~
. Date analyzed II* o 1-,Q Titrant normali pH of Normality Deionized Titrant check Begin water reference standard ml = 4.5 S.U. number number Reference standard True value number (TV) (mg CaCO:YL) 100 Sample volunie ml 100 Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Titrate samples to pH= 4.5 S.U. Page __ _ Page ---.:; __ of 5 Time initiated
(mgi'
__ ---1 Time completed
                                                        .1,
.....
                                                      ..co..
Normality (N) ofH1S04 pH Factor or Multiplier End Total = (S ml Na1C03 x 0.05)/E = (N x 50000)/100 ml sample =NxSOO ml ml = 0.25/E Begin End ml ml 12.1> 21. (acceptable range= 0.0180 -0.0220) 0*020 Total in I Alkalinity (MV) Multiplier (mg CaCO:YL)
Sample volume (ml)
Alkalinity Multiplier (mg CaCO:YL) s D % RS= MV /TVx 100 (acceptable range = 90 to 110&deg;/o) oJ .. %RPD= {(S-D) /((S+D)/21}
Begin ml
x 100 (acceptable range= :1:: 10%) Begin End Total Multiplier Spike alkalinity (A) (mg CaCO:YL) ml ml ml lOO .2.o.J..?
                                                                                              /
3.< .5 ro.o \t> .4-Sample alkalinity (B) Measured spike value (MV) % R=MV /SVx 100 (mg CaCO:YL)
End ml Total ml Multiplier Alkalinity (MV)
MV=A-B (acceptable range (mR CaCQ:YL)  
(mg CaCO,VL)
=75 to 125%) 5? 5o \Cocfo s l amp, e measurements:
                                                                                                                                                    % RS-MV /TV x 100 (acceptable range
Sample volume Begin End Total Alkalinity Sample number Sample ID . (ml) ml ml ml Multiplier
                                                                                                                                                          =90 to 110%)
_(mg CaCO:YL) ll-02..(0 ".,0 \DO*  
100          ~-
!Q.O \o.&#xa3;4-lo2 \\ ._()'5. LD I ,;g_s IAA-4 s.q La I l\*01. LO \ 1'\.0 (o.o tq,O to1. \I.D 1-lOA Sa..U sw \11.0 IS.v q_c Cl4 I\.Dt.l0.8 I 15.o u.os.lu \ 10-l I
I
s.CJ lil 10110'-1*
                                                                                "'B~
crt I z.
ll11nll,.,.,,.. sample                     ...
41-A q,; Ct'l-1.011 oc,.o 1.-J 3 o.o o..s q.c;; 1.., Cft:t Reviewed by:
Sample                                                       Alkalinity                 %RPD=
{(S- D) /[(S+D)/2]} x 100
                                                                                          ~~
Sample                      Sample ID              volume                            Total  Multiplier      (mg CaCOJt'L)
I
                                                                                                                                              ~
number                                                (ml)                              ml s
I Duplicate (B)                                               "'                         D I
Mall'ix spike '"'""""''J*
Reference standard number Spike value (SV)
(mg c ..co.JJ ,\
Sample volume (ml)
Begin ml End ml Total ml
                                                                                                                  ~.
Spike alkalinity (A)
(mg CaCOJIL)
I                                                    Sample alkalinity (B)
(mg CaCO,VL)
Measured spike value (MV)
MV=A-B (mg CaCn./1.\
                                                                                                                            "' "' *A, R~L/ sv X 100
                                                                                                                                        '7Sto~)~ ..
I        sample measurements:
I          Sample number                           Saii!Pie ID Sample volume (mil Begin ml End ml Total ml         Multiplier Alkalinity
_ime CaCO,VL) lb.toz.t.o1.-               i'"'l'A .i&#xa3;.r.~wltl z.                       IOU             J~~ 4{)0,         4.~        J0-8.                   '-({!;
I                                                           I                                   'lo.tf t./5.~      '-/.-;                                 Lfti lo1o~"'
                                                                    .3                I                                              (
                                                                                                                              ~
I                                                                                                  I~
                                                                                        ~        rv*
I                                                      ~
                                                                      ......... ~
I                                -      ......--:.
Reviewed by:                                     Date reviewed:     I   I0~\-10 I
j Page67ofl05 SOP C6- Exhibit C6.1, revision 09-01-09
 
              *ers
              )
                )L_
I            i*~**'
                                  .     ,.-.J ErMronm~taiTestfngSalutlons,lnc.
Page _ __
Page ---.:;__of        5 Alkalinity (SM 2320 B)
Analyst Date analyzed I  ~St..
II* o 1- ,Q Matrix: Water, RL = 1.0 mg CaC03/L Titrate samples to Time initiated ~--.!.\.:..:\Q~C;,__ _---1 Time completed .....___;.1-3....:.;:o:;...~----'
pH= 4.5 S.U.
Titrant normali pH of                                Normality                                      Normality (N) ofH1S04                       pH Factor or Multiplier Deionized            Titrant              check      Begin    End      Total       = (S ml Na1C03 x 0.05)/E                 = (N x 50000)/100 ml sample water          reference              standard      ml        ml       ml                   = 0.25/E                                 =NxSOO
              = 4.5 S.U.           number                number                                  (acceptable range= 0.0180 - 0.0220) 0*020 Reference standard                True value          Sample                                                      Alkalinity (MV)       % RS= MV /TVx 100 number                          (TV)        volunie    Begin    End      Total      Multiplier         (mg CaCO:YL)             (acceptable range (mg CaCO:YL)             ml          ml      ml      in I                                                    = 90 to 110&deg;/o) 100            100 12.1>    21.                                                                      oJ ..
Alkalinity                %RPD=
Multiplier        (mg CaCO:YL)        {(S- D) /((S+D)/21} x 100 (acceptable range= :1:: 10%)
s D
Spike alkalinity (A)
Begin    End    Total      Multiplier                        (mg CaCO:YL) ml       ml       ml lOO       .2.o.J..? 3.< .5 ro.o           \t> .4-Sample alkalinity (B)           Measured spike value (MV)                 % R=MV /SVx 100 (mg CaCO:YL)                       MV=A-B                               (acceptable range (mR CaCQ:YL)                             =75 to 125%)
5?                               5o                                     \Cocfo samp,l e measurements:
I            Sample number                            Sample ID Sample volume
                                                                                  . (ml)
Begin ml End ml Total ml         Multiplier Alkalinity
_(mg CaCO:YL) ll-02..(0                   ~~              ".,0                     \DO*       oz.~        ~~.s        !Q.O         \o.&#xa3;4-                 lo2 I            \\ ._()'5. LD l\*01. LO I
                                                \
                                                                                              ,;g_s IAA-4 s.q 1'\.0       (o.o       tq,O La I to1.
I              \I.D 1-lOA I\.Dt.l0.8 Sa..U sw I
                                                                                                \11.0 IS.v 15.o ~~~-1, q_c
                                                                                                                      '=~-*"'
Cl4
                                                                                                                                                          "=1*~
u.os.lu                           \                                             ~~.\o        ~.~        10-l                            ~lOb I            /0Jib2..~                  '"Pa~tnCLI'"l I                                       3;}..~      3~.1d        s.CJ                             lil 10110'-1* crt                                   I       z.                       ~Kt,      41-A         q,;                              ~ Ct'l-I            1.011 oc,.o 1.-                                J      3 Reviewed by:
o.o        o..s
                                                                                                                \~                                                                                                                        q.c;;             1..,
Date reviewed:
Date reviewed:
I 1\Page 68 ofl05 "*"1-*lO SOP C6-Exhibit C6.1, revision 09-01-09 I I I Analyst bate analyzed
Cft:t I   ~
( 1 . 01-*tO True value Sample number (TV) -volume (mg CaCOJIL) ml 100 100 Sample volume M t. ik a riX sm e recoverv:
                                                                                                                                                                  "*"1-*lO E
Reference standard Spike value Sample number (SV) volume (mg CaCOJIL)
\Page 68 ofl05                                                                                                                SOP C6- Exhibit C6.1, revision 09-01-09 1
(ml)
 
't '9) J()) Sample alkalinity (B) (mg CaCOJIL) 3li-l s am_]lj e measurements:
Page _2....,___
Page _2....,___
Page     Z.. of_,S=---
Page Z.. of_,S=---
Alkalinity (SM 2320 B)
Alkalinity (SM 2320 B) Matrix: Water, RL = l.O mg CaC03/L Begin ml '\.'5 Begin Begin ml Titrate samples to pH= 4.5 S.U. Time initiated I .............
Matrix: Water, RL = l.O mg CaC03/L Analyst  ~-~fDL.~"'------;                                                                    Time initiated    I .......
Time completed End ml ,q.o End ml End ml Normality (N) of H2S04 = (5 ml Na2C03 x 0.05)/E =0.15/E pH Factor or Multiplier
bate analyzed        (1. 01- *tO                          Titrate samples to pH= 4.5 S.U.
= (N x 50000)1 100 ml sample =Nx500 =0.0180-Alkalinity (MV) % RS= MV /TV x 100 Total Multiplier (mg CaCOJIL)  
Time completed      ~=====.......:;=======
(acceptable range ml =90 to 110%) ,o.<+ qq l:tttOfo Alkalinity  
Normality (N) of H 2S04                  pH Factor or Multiplier
*,t,RPD=
                                                                                            = (5 ml Na2C03 x 0.05)/E               = (N x 50000)1 100 ml sample
Total Multiplier (mg CaCOJIL)  
                                                                                                      =0.15/E                                =Nx500
{(S *D) /((S+D)/2)}
                                                                                                          =0.0180-True value        Sample                                                Alkalinity (MV)       % RS= MV /TV x 100 number                  (TV)          -volume    Begin      End      Total   Multiplier       (mg CaCOJIL)             (acceptable range (mg CaCOJIL)            ml       ml      ml        ml                                                  =90 to 110%)
x 100 range= :1: 10%) 3. *" leA-;.-; .l-Spike alkalinity (A) Total Multiplier (mg CaCOJIL) ml <o.2. IDH B'5 Measured spike value (MV) % R= MV /SVx 100 MV=A-B (acceptable range (mg CaCOJIL)  
100              100              ,q.o                  ,o.<+             qq                       l:tttOfo
=75 to 125%) 151 I 02.Pio I I I I Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mK CaCOJIL) loll "Z..
                                                                      '\.'5 Sample                                                    Alkalinity                 *,t,RPD=
* z.. \l)D 30.1..  
volume    Begin      End      Total     Multiplier     (mg CaCOJIL)         {(S *D) /((S+D)/2)} x 100 ml                                                                range= :1: 10%)
&.1-IO.Y. 3i J.D u_nc;. .u-4 . l 3 \
                                                                                ~-~      3. *"     leA-
31.ct 3.tt, 31-1 bll 01..0 I C'nO_iLll-l  
                                                                              ~5.1-
' a,<; 01-.q 4t2. ;,; y) 1'-10 10 llo'l. o\ I z. IJfL2, #.4 3.2 t3u J u I I 0 I. .(1!) I 3 \ I ILK) II ol.ot.l --r J A-PAt: i"x''' J 6D b,O 3.q (2) I 0 II I z. 3.'1 Q.J J.+:z, S-:f i 10 11 nc.o'\ I 3 9.1 1-\-.v 93 IOIIOI.oS "f(A fA,:' II'ST c \1-\ t:;.o . ..-\0\) ,. 10'-1 i ..
                                                                                          ;.-;       .l-M at.riX smike recoverv:
69 of I 05 Reviewed by:
Reference standard        Spike value      Sample                                                                Spike alkalinity (A)
I                number                  (SV)
(mg CaCOJIL) volume (ml)
Begin ml End ml Total ml Multiplier                       (mg CaCOJIL) t!:vv~<. 't ~'D                    '9)            J())  a~.LJ.    ~o.ft1    <o.2.     IDH                     B'5 I                                          Sample alkalinity (B)
(mg CaCOJIL)
Measured spike value (MV)
MV=A-B
                                                                                                                      % R= MV /SVx 100 (acceptable range I                                                                                  (mg CaCOJIL)                         =75 to 125%)
3li-                              151                             I 02.Pio sam_]ljl e measurements:
I           Sample number                 Sample ID Sample volume (ml)
Begin ml End ml Total ml       Multiplier Alkalinity (mK CaCOJIL) loll o~. "Z..
* Soru11oO~.                        z..     \l)D           30.1.. 34.~          &.1-         IO.Y.               3i I            J.D u_nc;. .u-4 1 bll 01..0 I         C'nO_iLll-l l        3 a,<;
                                                                            \          :1,4.~
01-.q 4t2.
31.ct 3.tt,
                                                                                                            ;,;     y) 31-1'-10 z.'
I              10 llo'l. o\
Ju I I   0 I. .(1!)
I I         3               \
IJfL2, #.4
                                                                                      ~-q.,      ~'+                                                                                                              3.2 3.~
I t3u ILK)
I~  II ol.ot.l     --rJ A- PAt: i"x'''         J     6D               b,O       3.q               (2)                       ~I Ii I 0 II n~. '2.~                    I I
: z.                       3.'1     Q.J
                                                                                                            ~B J.+:z,                             S-:f 10 11   nc.o'\                               3                         9.1
                                                                                                  '~-'      1-\-.v                             93
~
..i IOIIOI.oS           "f(A fA,:'       II'ST c Reviewed by:
                                                                                        ~-'      \1-\
                                                                                                      ~t'A-t:;.o             . ..-
Date reviewed:
Date reviewed:
I u.oJ.w SOP C6-Exhibit C6.1, revision 09-0 l-09 Analyst
                                                                                                                                              \0\)
.. .._----1 Date analyzed II* 0-:f*lO Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCOJIL Titrate samples to pH=4.5 S.U. Page 3 Time initiated .............  
Iu.oJ.w
-=-....,....----1 Time completed  
                                                                                                                                                        ,. 10'-1
. . Titrant normality an d [I'll d t mu ttp4 er etermmat on: otto! Normality Normality (N) ofHzS04 pH Factor or Multiplier Deionized Titrant Ranln End Total = (5 ml NazC03 x 0.05)/E = (N x 50000)1 100 ml sample water reference standard ml m1 _, =0.25/E =Nx500 -4.5S.U.
~age 69 of I 05                                                                                                    SOP C6- Exhibit C6.1, revision 09-0 l-09
number number (E)
 
.. e-0.0180
Page-=--
* 0.0220) --.. -*.*. -. :La oratory contro s an at'< : Reference standard True value Sample ' Alkalinity (MV) o/o RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mgCaCOYf,)  
Page        3      of~5=---
(acceptable range (mgCaCOJIL)  
Alkalinity (SM 2320 B)
(ml) ml ml ml =90to 110%)
Matrix: Water, RL = 1.0 mg CaCOJIL Analyst  ~c&s:;z<~...._----1                                                                            Time initiated ~.............
100 100 n*.l  
                                                                                                                                                            -=-....,....----1 Date analyzed      II* 0-:f*lO                                Titrate samples to                            Time completed .                ~
'l!\.
pH=4.5 S.U.
q"5 cts"/o Alkalinity o/oRPD = Multiplier (mg CaCOYf,)  
                . Titrant normality an d mu[I'll   ttp4 er determmatton:
{(S *D) /[(S+D)/2)}
otto!                       Normality                                           Normality (N) ofHzS04                     pH Factor or Multiplier Deionized     Titrant                           Ranln   End     Total         = (5 ml NazC03 x 0.05)/E                 = (N x 50000)1 100 ml sample water       reference       standard           ml       m1                             =0.25/E                                     =Nx500 number                                       (~an ..e- 0.0180
x 100 (acceptable range =:I: 10%) M. 'k atl"lx spl e recovery:
* 0.0220)
Reference standard  
                  -4.5S.U.       number                                              (E)
. Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mgCaC<M.)  
              .:La oratory contro s an at'< :                                                                                                                       -
(meCaCOJILl (ml) ml ml ml i:t"NSS &?>"D lDO 9) .;1.1-10.0 ('l) li:>.Lf 2.\0 Sample alkalinity (B) Measured spike value (MV) o/o R = MV I SV x 100 (mgCaCOJIL)
Reference standard       True value             Sample                   '                                   Alkalinity (MV)       o/o RS = MV I TV x 100 number                 (TV)             volume     Begin   End       Total     Multiplier           (mgCaCOYf,)               (acceptable range (mgCaCOJIL)               (ml)       ml       ml         ml                                                         =90to 110%)
MV=A-B (acceptable range (m2CaC(M.l  
:1:'-NSS~o 100               100 n*.l     ~~.)      'l!\.         \C.~                q"5                         cts"/o Alkalinity                   o/oRPD =
=75 to 125%) \\0 Jet. lt>octo s [i amo, e measurements:
Multiplier         (mg CaCOYf,)         {(S *D) /[(S+D)/2)} x 100 (acceptable range =:I: 10%)
Sample volume Begin End Total Alkalinity Sample number SampleiD (ml) ml ml ml Multiplier (mzCaCOJIL) ll\ II OS. lO 1-"'QAPAF lNf 3 5D 5'.'2-IN\
M.atl"lx spl'ke recovery:
1 'o -tua 10  
Reference standard       . Spike value           Sample                                                                   Spike alkalinity (A) number                 (SV)             volume     Begin     End       Total     Multiplier                         (mgCaC<M.)
''1"1 II' A AA 0.0 5'-0 s.v fOO .. ,()"\ I 0 Jl 01!.. '2i " \t.if c:tA 5.0 I \t) *' 110 .. ,o .. I oil 0 l.'S oo*L f.tP It> .I 15.o ,..._q too *lb2 J 0 I I * '1.4 .1NTAP In o I=) .tl.
(meCaCOJILl               (ml)       ml     ml         ml i:t"NSS &?>"D                         lDO             9)       .;1.1- ~-~          10.0       ('l) li:>.Lf                   2.\0 Sample alkalinity (B)             Measured spike value (MV)                   o/o R = MV I SV x 100 (mgCaCOJIL)                           MV=A-B                                 (acceptable range (m2CaC(M.l                               =75 to 125%)
* tl2. J 0 1\0l .tn. \1A .s&N tD\ ' .2n.c.l-(t) w (,1-1 oil 0"!..2.1 I 2 ":}, 10 11os. en 3 !a1-.o 30.3 a..:s l#CJ 1011 oi.Q'!,  
                                                            \\0                                   Jet.                                 lt>octo samo,[ie measurements:
)1\\"\ '
Sample volume       Begin       End         Total                                 Alkalinity Sample number                 SampleiD                       (ml)             ml         ml           ml       Multiplier             (mzCaCOJIL) ll\ II OS. lO       1-"'QAPAF           lNf 3                 5D             ~\-1'      J."to~      5'.'2- IN\ lo.~                        1'o       -tua 10 Ill\~ ''1"1       II'A~~Cb2 AA                                             0.0       5'-0       s.v                                   fOO       . ,()"\
3:2---(('':J Revtewed by: Date reviewed:
I0    Jl 01!.. '2i
I \l. cr:J-10
                                                            \t.if c:tA                         5.0     I\t) *'       ~.I                                110         . ,o..
* age 70 of 105 SOP C6-Exhibit C6.1, revision 09-01-09 I I I I I I I ) Environmental Testing Solutions.
I oil 0 ~ l.'S                       oo*L f.tP                               It> .I     15.o         ,..._q                               too       *lb2 J 0 I I 0~ * '1.4                         .1NTAP                             In o     IJo~          I=) .tl.                             1r~
Inc. Alkalinity (SM 2320 B) Matrix:*
* tl2.
Water, RL = 1.0 mg CaC03/L Titrate samples to pH= 4.5 S.U. Page _Y.._,___
                                      \1A .s&N tD\                           ~00              .2n.c.l- :~~.Lt                    (t)                     w J 0 1\0l .tn.
Page t+ of-.:5::___
1 oil 0"!..2.1                   I             2 '                        ~~\p l~.o
Time initiated Time completed
                                                                                                                      ~.2
""'""'"'
                                                                                                                        ~.4                                ":},
'--------1 Titrant normality and multiplier determination:
(,1-10 11os. en                       ~              3                         !a1-.o 30.3               a..:s                             l#CJ 1011 oi.Q'!,       ~A5Qil            )1\\"\ '
pHOI Normality Deionized Titrant checK li'nd *o* water reference standard ml ml -4.5S.U.
number number Laborato control standard:
Reference standard True value number (TV) (mg CaCOJI'L) 100 Sample alkalinity (B) (mg CaCQJI'L) s l ample measurements:
Total ml (E) Normality (N) ofH1S04 pH Factor or Multiplier
-(S ml Na1C03 x 0.05)/E = (N x 50000)1100 ml sample =0.251E =Nx SOO (acceptable range= O.u.uv -* Total Multiplier litl (1_1+ t-o.t} Multiplier Total Multiplier ml .... Alkalinity (MV) 0/o RS = MV I TV X 100 (mg CaCOJI'L)
(acceptable range =90 to 110%) q?>01 o Alkalinity
%RPD= (mg CaCOJI'L)
{(S-D) /[(S+D)/2)}
x 100 (acceptable range= :1: 10%) Spike alkalinity (A) (mg CaCOJIL)
.) ta.l \D.4 11"0 Measured spike value (MV) % R = MV I SV x 100 MV=A-B (acceptable range (m2 CaCOJI'L)
= 75 to 125%) * '0' l Qll)lo Sample volume Begin End Total Alkalinity Sam_l!le number SampleiD (ml) ml ml ml Multiplier (m2 CaCOJIL)
* ell os.()& ':-An ,,.sr 3 5D 1(1).1 11.3 a -'2-1\?.J lOA* Jo n \.01.. k'J'
\ /4.'5 -3.2.
IOJ l
: 2. \14-.S
?,.!3 lgq to II OS*0'1 3 l':f-.i lDC'J
,NtuJ, \ IJ4.l. 3.1 w JO 2'%. I 2. d4.J.. l;;r.,s :S.:z. lot\
/ 1 3 B1.'5 _3_._"-t
-:J-\ J 0 I i'fA 5Jttoa\ 10 r
\ lliCTAA 35.2 4-S .,;I....
'14 lage 71 of I 05 Reviewed by: Date reviewed:
I l l*U'1*.0 J SOP C6 -Exhibit C6.l, revision 09-0 l-09 
=er.,. &#xa3;.,, .. $ * ' J ._*. . r---' ) Environmental Testing Solutions, Inc. Analyst I P,-&(...
Date analyzed ll* OJ-I p water reference standard
= 4.5 S.U. number number Laborato control standard:
Reference standard True value number (TV) (mg CaCOJIL) 100 Sample alkalinity (B) (mgCaCoYL) s t amme measurements:
Page -=---Page 5 of_,S"'---
Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Begin ml 4.0 Titrate samples to pH= 4.5 S.U. Time initiated
,........_
Time completed pH Factor or Multiplier
= (N x 50000)1100 ml sample =NxSOO (acceptable range= 0.0180
* 0.0220) Alkalinity (MV) % RS = MV I TV x 100 Total Multiplier (mg CaCOJIL)
(acceptable range inl = 90 to 110"/o) cvt 95f0/o Alkalinity 0/oRPD= Multiplier (mg CaCOJIL)
{(S. D) /[(S+D)/2]}
X 100 (acceptable range= :1: 10%) \0!-1-s j_ D Spike alkalinity (A) End Total Multiplier (mg CaCOJIL) ml ml CJ.o a.
\qo *Measured spike value (MV) % R = MV I SV x 100 MV=A-B (acceptable range (me:CaCoYL)
=75 to ll5%) 105 l D'\:5&deg;lo Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOJIL)
I \l ll .llo
'5D 13-C 1::1.\, 4.\D lti) lC-4-9{p IO*.&b*l mi-\C.. u u \ 100 S.<l. Ito 11*J.H **o J 2.. I ..23.4 act.'2.. Lzc ll*C"Z.*IO f 3 \
35.1 s.g_
!GoA,'\l,"'GJ Bea.tn as.1 S.-3 1-I tO _<" / / / ./J. "' Reviewed by: Date reviewed:
I i\-a-:l*&O 72 of lOS SOP C6 -Exhibit C6 .1 , revision 09-0 l-09 I I :_*cy, . ;;!j _j Envfronmentol Tesdng SalulloM,Inc.
Analyst ,l-f/b;"-""""-=-----1 Date analyzed (C)*'31.1V Lb I tJ d. a contro stan ar :
* Reference standard True value Sample number (TV) volume (mg CaCo,/L)
(_ml) 40 50 Sample Page CfCJ. Page __ l of ,_ Total Hardness (SM 2340 C) RL = 1.0 mg CaCOJI, Begin Enct' ml ml ,,1 Time initiated I
Time completed Normality (N) of EDT A =0.2/E pH Factor or Multiplier
= (Nx SOOOO)/ SO ml sample =Nx 1000 (acceptable range= 0.0180 -0.0220) c1. Hardness (MV) % RS = MV I TV x 100 Total Multiplier (mg CaCo,/L)
(acceptable range *ml =90 to 110%) 2.D z., 1..{(.) j(J, y ll>.l)
* Hardn(!SS
%RPD = Sample Sample ID . volume Begin Multiplier (mg CaC()JIL)
{(S-D) /[(S+D)/2)}
x 100 Matrix spike 1')1: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL)
. (mg CaCOJIL)
(ml) ml ml ml 11\i 40 60 l'f.O J9.() &../.o Sl Sample hardness (B) Measured spike value (MV) % R=MV /SVx 100 (mg CaCO.YL)
MV=A-B (acceptable range (mg CaC()JIL).
=75 to 125%) L(Z,. 3t) QfJ:S I. s l ample measurements:
Sample volume Begin End Total Hardness Sample number SampleiD (ml) ml ml ml Multiplier
{me: CaCO.vLl TV=ND Blank (should be = 0 mg CaCOVL) 61J 0.0 0.0 o,o NO 10 J..j_'()
18.0 10 . .30
* IO C\
4.4 lo *3CHO B
_g , " -i.'S' ClJ a 31.u a,., "i. S' Of I
.0 8 ' Jll.l l"f.S 0, I 10/Dl.la.
(J I n w1 , 1ov '/1/j t{._3 ZJ_ I% I_DJf.l_'2.L*
o I ' z. l 1'\.1 R.D t.( ,'i I /q8-d,IJ{j IOJO!Q.(A I 3 ltl.o )Iff. '-1 s.'i ' A lfl_ * ,Ltt, I OIO 2.C. .n,_ T'"" v1 e &AJJJJ..
t AY*1 5 " , I .A-/Z, Note: If >!Sml of titrant is used, sample must be diluted.
Reviewed by: IL----I.ZI:;.,.l,..
_ ___. Date reviewed I ffJ*
Page 73 of 105 SOP C7 -Exhibit C7 .I, revision 09-0 l-09 I I I I I I I I I !E ... , J, .. S" .. ,, .... ,, .. l7. .. ',
* Environmental Testing Solutions, Inc. Analyst I Date analyzed Total Hardness (SM 2340 C) RL = 1.0 mg CaC03/L Total ml ml number Sample Sample SampleiD volume Begin ml Duplicate (B) Matrix Reference standard Spike value Sample number (SV) volume Begin End Total Hardness (MV) Multiplier (mg CaC<>JIL) o/oRS=My/
(acceptable range =90toUO%)
Hardness
%RPD= (mg CaC<>JJL)
((S *D) /[(S+D)Ill}
x 100 Spike hardness (A) Multiplier (mg CaC()JIL)
Sample hardness (B) (mg CaC03iL)
Measured spike value (MV) MV=A-B s l ampje measuremen ts : Sample volume Begin End Total Hardness Sam_IDe number SampleiD (ml) ml ml ml Multiplier (mg CaCOJI'L)
Blank (should be-0 mg CaCO:YL) 11 /(}10"2&sect;.
.en--rv A uno wtiJ. ,. 50 40
.04 I 3 I 3D:#" ;..a.-t.f'1 ------\. v--r 1------..........:::::
Note: If >ISm! of titrant is used, sample must be diluted.
Reviewed by: Date reviewed I /o*.3J*IO Page 74 of 105 SOP C7 -Exhibit C7 .1, revision 09-01-09 l l _I l _I I ] -J J J 1_: I u-u L iETS Envlroomontaf Testing Solutlonr,lnc.
Analyst Date analyzed
* 10 Titrant reference number Sample ID Begin End Total ml ml ml o.o Normality (N) of EDT A =0.2/E (acceptable range= 0.0180 -O.OllO) o .o2.o tq Page__._
__ Page _.!__of 5 pH Factor or Multiplier
= (N x 50000)/ SO ml sample =Nx 1000 2c."' Hardness (MV) % RS = MV I TV x 100 Multiplier (mg CaC03/L) 2-o. \i' 3 Hardliess Multiplier (mg number End Total Multiplier Spike hardness (A) (mg CaCO]/L) amp1 e measurements:
Sample hardness (B) (mg CaCOJIL) 31-ml ml Measured spike value (MV) MV=A-B Sample volume Begin End Total Sample number Sample ID (ml) ml ml ml TV=ND Blank 6D (should be = 0 mg CaCO:YL) 1\'. .s \'P.5 O.Q \\.oz..,c m\-\5 tho lf.S 1\*D\*lO '"' n '2. tf'J!.
tr'Q ("\ \
M.l lo II b'-t
* V1 \ 2 c:il.'5
\ 5 /0 IIOLO l SoiU.l.U' Pihi \
l4._k l.'l I b It O'lt. 'Z.b I z. .40.1n 10 u os. ol./ \ 3
).q %R=MV/SVxlOO Hardness Multiplier (mK CaCO]/L) 2S).'f;l
).Jp I C\3 -
-.....--'5Z. 52.. l{3 3'\ 31 __._ 3G\ l: Note: If >ISm! of titrant is used, sample must be diluted.
Reviewed by: '-1 .....:.ll'o+=--..:._:
___
Date reviewed I ll*O l* ,u Page 75 of lOS SOPC7-ExhibitC7.1,revision09-0l-09 
.. ETS ** * * * ,,,,
'''1,:.-\' Envfronment*l Testing Page -'z..::::....__
Page .ri Total Hardness (SM 2340 C) RL = 1.0 mg CaC03/L Time completed
= "'""'"4 Analyst l Date analyzed 1-1)0 ... o:;..c_i-_*_i_0_--1 Time initiated r Titrant normality and multiplier determination:
..........
-"* t'he .. k Begin End Total Normality (N) of EDT A pH Factor or Multiplier reference standard ml -* =O.l/E (Nx 50000)150 ml sample Ull number number (E) (acceptable range
* u. !' =Nx 1000 True value Sample Hardness (MV) o/o RS = MV I TV x 100 number (TV) volume Begin End Multiplier (mg CaCQ,IL)
Mi ix ik atr &#xa5;1 erecove r.r: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End TotaJ Multiplier (mg CaCOJil,)
(mg CaCQJIL)
(ml) ml ml ml
<b11-40 5b t<g f).<; 3.1-'" Sample hardness (B) Measured spike value (MV) o/o R = MV I SV x 100 (mg CaCOJIL)
MV=A-B (acceptable range (mg_CaCQ,IL)
=75 to 125%) 6'\
) s l measurements:
Sample volume Begin End Total Hardness Sample number SampleiD
_(ml) ml ml ml Multiplier (me: CaCQ,/L)
Al,.nlr (should.be
= 0 me: CaCO]/L)
I 0 H O'l..O l CJ:..iJ IL iJrt \ 5.5'" #-f.3 &#xa3;i) 2.D.'-IJ
&1(0 JU II b'-1.01 t 2-I l?>.'i I I 10 II Of..((,!,
' . .2 l3ll tt4 3.5 I Jb 11 o \.o'i -.v A A:\i=tn\
' }0 11-.4 IJs.<l. 'a.li-I_D I z ..25$ co .t; I ta'ao
* 0':lt. 101lOS. O'l \ .3
-:J.) I
/OitOI*OS hJ A PAf l a6 .&fl.'+ 1\0 -l\1 _l_O
\ z. \ 1--H.\ I \20 lbaJOS.ro I .) I H\p.q 8.0 I
\ '2o -
t, Note: If >15ml oftitrant is used, sample must be diluted.
Rev1ewed by: l..__ __
Date reViewed I \ \.\)1-*10 'i Page 76 of 105 SOP C7 -Exhibit C7.1, revision 09-01-09 I I I I I I I I I I I I I I I !ETS EnvlronmontaiTulfng Solutions.
Inc. Analyst I Date analyzed D:.:1:...._*l-0---1 in Titrant normality_
and mu er 'eterm ation: Ti ....... nv* ouMuy l:tun:ft.
u;:1m I:IUU reference standard ml ml number number (E) control Reference standard True value Sample number (TV) volume Begin End End ml Mi ix lk atr sp1 e reco-very:
Reference standard Spike value Sample number (SV) volume Begin End (mr CaC<>JJL)
(ml) ml ml .:t'NSS <62-:t lb (c..2. Page _,3::o..__
Page 3 of_,5=----
Normality (N) of EDTA pH Factor or Multiplier
=u ** ,_ -(Nx 50000)150 ml sample (acceptable range 0.0180. 0.0220) 1\T.., 1000. Hardness (MV) % RS = MV I TV x 100 Total Multiplier (mg CaC<>JJL) z.c."' 3<1 Hardness Total Multiplier (mg CaC{M..)
ml Spike hardness (A) Total Multiplier (mg CaC031L) ml 3.0 f)) ').J) . "' ;3lo Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOliL)
MV=A-B (acceptable range (mr; CaC03fL)
=75 to 125%) \'Z..o 1qo s l ampie measurements:
Sample volume Begin End Total Hardness Sample number SampleiD (ml) ml ml ml Multiplier (mr; CaC03fl,)
TV ND Blank (should be-0 mg CaCO:VL)
-"
JJ:ll' 2.. 1JA Pfh:"oo,,
tJTM (t.2. G.? 3.1 1.D.\a ' ao . t'Z.S _l_O_IL 00'2. AP I'D 'l.; to.s Ll.. (S) ''2.-0 -\ I 0 I I b1a.
'NJ Rf as; 10-'5 )-=\,!:)
21.0 (() 1'2.-o ...
to IIO\.a1-SG.n \ 5'D 13.':5 13_.\) 8.'5 1-2. /Jj_}l ()'!!,. 2.\ I z \t.O
-:}() .1n 1 1
\ ..3 ..
?..c; ?2. Jo frJA SQn
'
J':l-l 3.; 1Il I \ m:t.Ot. I 3.'+ ":fo _10 uos.. 0 f ' _, l 30.\o 3.Y. ":fO I Note: If >lSml of titrant is used, sample must be diluted.
Reviewed by: I Page 77 of 105 Date reviewed I \\ .o 1* SOP C7-Exhibit C7.1, revision 09-01-09 I _ _.tp.m-"
*--I I I I I I I I I I I .ETS * * * . ., -'"** .... _,,.,---EnvlranmentaiTestlng Solutions, Inc. Analyst Date analyzed 1\ *Ct *10 Titrant normality and multiplier determination:
T3 -" check Begin End Total reference standard 1111 number number (E) ix ik Matr sm e recovery:
Reference standard Spike value Sample number (SV) volume Begin End (mg CaCQ31L)
(ml) ml ml "lO SJ Cfl.z, l*tb Page _J.f. __ Page J.f of-=5'----
Normality (N) of EDTA pH Factor or Multiplier
( acceptame Total ml =O.l/E = (N x 50000)150 ml sample -1Ul110\
= N..x 1000 ---.-Hardness (MV) Multiplier (mg CaCO]/L)
% RS = MV I TV x 100 (acceptable range =90 tollO%) Multiplier 2.0.\p Hardness (mg CaCQ31L) s {(S-D) /[(S+D)/l)}
1 100 Spike hardness (A) (mg CaCO]/L) 1\0 I Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 I I I I I (mg CaCO,!L) 1-0 s. l amp* e measurements:
Samole number SampleiD TV-ND Blank Ill\\ en,. 'Z.\ 11 A 57!\(\ luh.>u z 1o 01 I IO 1J A-5/\.f\ l tS'ii)J 1 /D II ()!J.1.'1-I 2. lo IJ OS .oR \ ...a Jo AA J 0 I I 0!..1'-l t,JTAA lllU 61). IS 00\ PrP bJTftP Sample volume -(ml) so MV=A-B (acceptable range (mg CaCO]/L)
=75 to 125%) JfD toct>lo Begin End Total Hardness ml ml ml Multiplier (mR CaC03/L)
L at&:o 3 .'5 2..\).\9 "12. 0.0 3.\1 6.\Q i'+
q,J 3.'5 ":1'2.. 1-:t. \ lO.S .3.4--=to ,0$
a.cg P+.3 2.0.5 u.-2. t3o -lc.S lt.'\ 14-0 ..
l30-l2.e, 03.\o 3'-l-':t -L--
I Note: If >15ml of titrant is used, sample must be diluted.
Revtewed by:
Revtewed by:
Date revtewed I L\.(13 .jQ r Page 78 of 105 SOP C7 -Exhibit C7.1, revision 09-01-09 I I I I -I I
1~.3        ~~.'5        3:2-Date reviewed:
* I
                                                                                                                                                          ~ -(('':J I    \l. cr:J-10
* i
* age 70 of 105                                                                                                                SOP C6- Exhibit C6.1, revision 09-01-09
* I iETS ..:) Environmental Testing Solutions, Inc. Page _,5::...___
 
Page o Total Hardness (SM 2340 C) RL = 1.0 mg CaC03/L Analyst /J--L.:aillos.C:c.,::;.
Page    _Y.._,___
Page      t+      of-.:5::___
            )  Environmental Testing Solutions. Inc.
Alkalinity (SM 2320 B)
Matrix:* Water, RL = 1.0 mg CaC03/L Time initiated        ~-'::==-.....;:::,.._::::::--,--1 Titrate samples to                        Time completed                        ""'""'"'
                                                                                                                                                        '--------1 pH= 4.5 S.U.
Titrant normality and multiplier determination:
pHOI                                  Normality                                      Normality (N) ofH1S0 4                      pH Factor or Multiplier Deionized            Titrant                  checK          *o*
li'nd  Total        - (S ml Na1C03 x 0.05)/E                  = (N x 50000)1100 ml sample water          reference              standard        ml      ml      ml                  =0.251E                                      =Nx SOO
          -4.5S.U.            number                  number                          (E)  (acceptable range= O.u.uv -
* Laborato control standard:
Reference standard                    True value                                                                  Alkalinity (MV)          0
                                                                                                                                                      /o RS = MV I TV X 100 number                            (TV)                                        Total      Multiplier        (mg CaCOJI'L)                (acceptable range (mg CaCOJI'L)                                        litl                                                      =90 to 110%)
100                                        (1_1+        t-o.t}              q~                            q?>0 1o Alkalinity                    %RPD=
Multiplier        (mg CaCOJI'L)          {(S- D) /[(S+D)/2)} x 100 (acceptable range= :1: 10%)
Spike alkalinity (A)
Total      Multiplier                          (mg CaCOJIL) ml
                                                                                          .)  ta.l          \D.4                            11"0 Sample alkalinity (B)          Measured spike value (MV)                % R = MV I SV x 100 (mg CaCQJI'L)                      MV=A-B                              (acceptable range II samplel measurements:
                                                                ~9 (m2
Test in,{orination:
Test in,{orination:
Randomizin_g template:
Randomizin_g template:       (t~~
Incubator number and .3-.= shelf location:
Date and times organisms                                                      Incubator number and I                                                  \\* 0\'"&0      \\oC)()
Artemia CHM number: _ c.t\ lo\ Sl l Drying information for weight determination:
were born between:                                                              shelf location:              .3-.=
Date I Time in oven: Initial oven temperature:
Organism source:                 A"TOY- 6.-m.\-\ ~,.., a\-0\-.o                Artemia CHM number:       _c.t\ lo\ Sl l Drying information for weight I                                                                                              oc determination:
Date I Time out of oven: Final oven temperature:
Transfer vessel information:
Total drying time:
pH=
                                                        ,.,'l    S.U. Temperature=
                                                                                      "'1~.1.
Date I Time in oven:
Initial oven temperature:
l\-o~*lO
                                                                                                                                    ~~*t_.
IH~
I                Average transfer volume:
: o. &e,"("/ -4.
Date I Time out of oven:
Final oven temperature:
l\*&O*tt)
l\*&O*tt)
IUS \oO'C:.
                                                                                                                                \oO'C:.
MHSW batch used SOP A T21 -Exhibit A T21.1, revision 04-0 1-09 Page 85 of 105 I I I I I I I I I I I I I , I I I I I I *ers * * * .,,*.,: *.,.' 1_,_, ,,_.,,.:,_ Envtnmm .... I'!Utlng In<. Specie,s:
IUS Total drying time:          "1.'-\-~o\J.t.C:,
Pimeohales oromelas Day l,;ORtrOI A B c 0 lo IO IQ 1 lb 10 IC 2 to 10 IC 3 /0 10 10 4 /0 10 10 s to 10 (C) 6 10 ll) ll) 7 to 10 10 A= Pan Traycolor
I                       Daily feeding and renewaJ information:
-"*
Day                  Date      Morning reeding            Afternoon reeding                                      MHSW I                                                                                                                                 batch used 0
.... IO B =Pan + Larvae weight (mg) lt.o tt. t-t.V{
I                 2 I                 3 4
Analyst:
I I
M Date: \\ .\O lO C = Larvae weight (mg) =B-A ,.OC\ , .... &.o\.
I I
per inidal number A>o.. ... o'-oflarvae
IPage 85 of 105 SOP AT21 - Exhibit AT21.1, revision 04-0 1-09
{mg) = C I Initial number of larvae b' tY ()' Average Percent weight per reduction initial from control 0."'\ 'L, number of {%) larvae (mg) D IO /Q It;, I() IO (0 10 c..1."!>
 
"'c., ... ()' Page 2 of5 PpKCICR Test Number: Survival and "-
I I
4SJ) m1
          *ers
* KCIIL ml! KCIIL E F G H I J K L to /0 IO IO /Q to IO IO to /C) 10 /0 IO /Q 10 I C) 10 IQ 10 lO ro 10 IO 10 I() ' 10 10 /0 "'"" . '\ ,r.\_ /0 ID 10 I(:) IQ '\ '\ C\ IO IC 10 I() JQ <\ " q ro I() IC {Q IO 'i '\ '\ /Q 10 10 /C rob-'\ '1 i *crt 10 H.t\
          ~ .,,*.,: *.,.'   1_,_, ,,_.,,.:,_
lfl.\1\ \'-l.ut t.tt.IC.
          ~ Envtnmm....I'!Utlng Soludon~ In<.
t:f...(.':\  
Page 2 of5 I                        Specie,s: Pimeohales oromelas                                                                     PpKCICR Test Number:                ~
'l,l .<< 1-z-1 .t'\) t4.il," l'lA .'H.  
I                                Day                                 l,;ORtrOI Survival and "-
&*"\'-,. t;.\ , ... '-... ,, ,.1.'-\ " \o'\ o* 0' o* C)' ()* ()' \)' ()* o. tc:,o -11...1\'7.
4SJ) m1 *KCIIL
0-11.'5 0 Coroment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg =unusually large, d&r = decanted and returned, w = wounded Calculations and data reviewed:
                                                                                                                        .::~Data
J{_ Comments:
                                                                                                                                              ~QQ ml!    KCIIL A        B        c        D              F                                                              L I                                    0 lo      IO        IQ    IO      to E
SOP A T2l -Exhibit A T2l.l, revision 04-0 l-09 .:Page 86 of 105 I I I I I I I I I I I I I I I I I I iETS Page 3 of5 Envlronmentll'lUtlngSolutlons.lnc.
                                                                                                        /0 G
Species:
IO H
Pimeohales oromelas PpKClCR Test Number: l'Z.O Survival and Growth Data Day 750ml KCIIL 900 mt: KCIIL lOSOm KCIIL M N 0 p Q R s T u v w X 0 /0 /0 10 lO IO 10 IO /D /0 IO (Q ID 1 IO ca u( t:t*l\ q*c( dul &M 411.( lo 1ilj 14 16.( a t 2 '\ '\ a'.l '-'lA fo--w. cflJJ.
IO        /Q I
5'4 lolA ' , 3 '\ &'J... (\ i " -\, to 'i"'-
to J        K IO        IO 1
510\ s 4 -,"'"' & '\ & \,. \o '-1. 5 5 c.[ 'all "' '-!lit ,.c, l" 3'U.. "'\ f i 5 lo 2 6 \,'lA .,vA. \ .. ,, \u. \ R "" '-l "'L 7 ..... '1 ... \St'. -e-, i (,. ., '"\ '2. \ A= Pan  
to I                                    2 lb to 10 10       IC IC /Q It;,      10 IQ
\?*13 "*""
                                                                                                        /C)       10 10
\'\.\;
                                                                                                                            /0 lO        ro IO    /Q 10 10 IO I C) 10 I                                    3 4
Trayc:olor
                                                            /0      10        10      I()       I() ' 10          10        /0
\?rl\ "* \1..'\\ \1..* Analyst:
                                                                                                                                                . t\~ '\ ,r.\_
1 Date: '\ \.. tn.. \0 B-Pan + Larvae weight (mg)
                                                                                                                                                                        /0
: 11. '6?>
                                                            /0      10        10      IO        ID      10      I(:)     IQ            '\      '\         C\       IO I                                    s to                (C)      (0                                                                  q            ro I
ll..ltf lb.1o 11.d l.l).o1 Analyst:
6 10 10 ll)      ll)     10 IC I()
M H::k Date: 1-\. to. lO C =Larvae weight (mg) '!t,l.l =B-A s.os Sf\'-
10 IC I()
$.()'-
{Q JQ IO
11>-"1 ,..1.1,.  
                                                                                                                                      <\
'*'-'-\.0'\ O.lo'\ \.i:t Weight per Initial nnmber " 'b'Vt, J.
                                                                                                                                        'i      "'\         '\          /Q 7
'" oflarvae (mg) o'-flt.."' "3-,'V = C I Initial number of larvae t; It) .., o* o* lb* o* o* ()' \:)' ()' Average Percent weight per reduction o.5l.<<o 'l..1*1 7. Q.3'-t\ S'!. *'2.7.
to      10      10        ~cr 10 10                /C        rob-        '\        '1      i  *crt      10 I                A= Pan weig~~~!'la. \l~
rL'\1o Initial from control number of (%) larvae (mR) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.
Traycolor -"*
Calculations and data reviewed: Comments:
                                                          \'1~ ,~.'tS'      t'--''~ \"\.'~\ ~~~      H.t\      \~1;,    \~*~ ~~\)\\ \~}.\~ lfl.\1\ \'-l.ut
SOP AT2l -Exhibit A T2l.l, revision 04-01-09 Page 87 of 105 -
                ~~;st\r.~.... IO I                 B =Pan + Larvae weight (mg)
Qo Qo ..... * * *
Analyst: M lol-~                      lt.o  tt. t-t.V{ ~l.'\!1 &#xa3;0.~ t.tt.IC. t:f...(.':\     u.~      'f,.~.'ll  'l,l  .<< 1-z-1 .t'\) t4.il,"  l'lA .'H.
* r:fY Environmental Testing Solutions, Inc. c ...........  
Date: \\ .\O lO I                                                                                                                                            ,...'- ...,, ,.1.'-\
(,..tL .........
C = Larvae weight (mg)
KO) A Cootrol B c D E F 450 G H 1 600 J K L M 750 N 0 p Q R 900 s T v v 1050 w X Duaett'o MSD value: PMSD: Iollillo-or J'IMI*amiM:rr*
                  =B-A
...... ...... 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 9 10 9 10 8 10 10 10 7 10 8 10 6 10 7 10 3 10 4 10 4 10 4 10 2 10 I 10 I 10 2 -0.1053 14.5 A*Puwtipl (IBI) 13.83 13.75 13.66 14.31 13.75 14.21 13.58 14.53 14.04 13.44 13.69 14.07 13.59 13.77 12.91 14.40 12.94 1323 13.79 12.64 13.41 14.13 14.82 13.71 MSD= PMSD= _J Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Metbod 1000.0 Quality Control
                                                          ,.OC\    ,.... &.o\. c..1."!> &'-~' &*"\'-         ,.,~      ~\t        ,. t;.\
--..... -Verification of Data Entry, Calculations, and Statistical Analyses Testaomber:
I                ~~ight      per inidal number
PpKCICR#IllO T .. t dates: November 02-09,2010 I'
                                                                      ...        o'-
'.A B*fu+Lanot LarYoewdpt(IIJI)
                                                                                                                                                                "        A._~
Wdgld I S.nlylac McuwdPU Cotllideat or varllllioll Wdpt/laidllloolllllu MeuiRI'ViYal MouweiPUioldll -*--wdPI(IIJI)
C)'~"" ()*"~'
*A-B oolllbcrorlorne(llll)
                                                                                                          ~'~._.
-**-beror
oflarvae {mg)
<M-..........
                  = C I Initial number of larvae A>o..  ~
_... orW.oe(llll)
                                                                                ~
(%) ....
                                                                                      ..."'c.,  ~\      ~        ~'->                            ~          \o'\
vll'ialloo(%)
()'                o*    0'       o*                            ()'        \)'          ()*
coatrol(%)
,I                                                        b'      tY                  ()'
...... , ... _.....,...._)(%)
Average                  Percent weight per initial number of reduction from control
(IIIII 20.92 7.09 0.709 0.709 21.44 7.69 0.769 0.727 10.9 0.769 100.0 0.727 21.72 8.06 0.806 0.806 10.9 Not applicable 20.56 6.25 0.625 0.625 22.16 8.41 0.841 0.841 22.67 8.46 0.846 0.850 6.0 0.846 100.0 0.850 6.0 -16.9 21.53 7.95 0.795 0.795 23.71 9.18 0.918 0.918 21.55 7.51 0.834 0.751 21.10 7.66 0.851 0.805 5.5 0.766 90.0 0.725 9.9 0.4 19.86 6.17 0.771 0.617 21.71 7.64 0.764 0.764 18.64 5.05 0.721 0.505 19.73 5.96 0.745 0.154 6.7 0.596 70.0 D.5l6 8.9 27.7 17.88 4.97 0.828 0.497 19.46 5.06 0.723 0.506 16.19 3.25 1.083 0.325 16.70 3.47 0.868 0.919 13.0 0.347 37.5 D.341 6.3 53.2 17.01 322 0.805 0.322 16.32 3.68 0.920 0.368 15.07 1.66 0.830 0.166 15.17 1.04 1.040 0.875 17.2 0.104 15.0 0.132 0.0 15.51 0.69 0.690 0.069 81.9 15.59 1.88 0.940 0.188 --*-Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precisioo.
{%)
The PMSD is the minimum percent differeru:e between the control and treabnent that can be declared statistically sisnificaot in a whole effiuentwxicity test. LowerPMSD bmmd detennined by USEPA (lOth percentile)
0."'\ 'L,                             o. tc:,o          -11...1\'7.          0-11.'5              0 .~?.
= 12"/o. Upper PMSD boWld detennined by USEPA (90th percentile)=
I                larvae (mg)
30%. Lower and upper PMSD hoWlds were detennined from the I Otb and 90th percentile, respectively, of PMSD data from EPA's WET lnterlabonllmy Variability Study (USEP A. 200la; USEP A. 2001 b). ' I USEP A 2001a, 200 I b. Final Report: lnterlabonllmy Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix.
Coroment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg =unusually large, d&r = decanted and returned, w = wounded I                                                                                                                                 Calculations and data reviewed:          J{_
EPA-821-B-01-004 and EPA-821-B-01-005.
Comments:
US Environmental Protection Agency, Cincinnati, OH.
I I
Qc 'C .... iETS Statistical Analyses
I
;) Environmental
.:Page 86 of 105 SOP AT2l - Exhibit AT2l.l, revision 04-0 l-09
'JesUng Solutions, Inc. Larval Fish Growth and Survival Test-7 Day Survival Start Date: 11/212010 TestiD: PpKQCR SampleiD:
 
REF-RefToxicant End Date: 11/912010 LabiD: ETS-Envir.
I                                                                                                                                                                       Page 3 of5 I
Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol:
iETS
FWCHR-EPA-821-R-02-013 Test Species:
          ~ Envlronmentll'lUtlngSolutlons.lnc.
PP-Pimephales promelas Comments:
I                  Species: Pimeohales oromelas                                                                                 PpKClCR Test Number: l'Z.O I                                  Day Survival and Growth Data 900 mt: KCIIL 750ml KCIIL                                                                          lOSOm KCIIL u                     w I                                    0
Conc-ml!!!:
                                                        /0 M
I 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 600 0.9000 0.9000 0.8000 1.0000 750 0.7000 0.8000 0.6000 0.7000 900 0.3000 0.4000 0.4000 0.4000 1050 0.2000 0.1000 0.1000 0.2000 Transform:
                                                              /0 N
Arcsin Square Root Rank !-Tailed Number Total Conc-ml!i!:
10 0
Mean N-Mean Mean Min Max CV"Io N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 600 0.9000 0.9000 1.2543 1.1071 1.4120 9.935 4 12.00 10.00 4 40 *1so 0.1000 0.1000 o.9939 o.8861 1.1011 9.086 4 10.00 to.oo 12 40 0900 0.3750 0.3750 0.6584 0.5796 0.6847 7.979 4 10.00 10.00 25 40 *1050 0.1500 0.1500 0.3927 0.3218 0.4636 20.862 4 10.00 10.00 34 40 Auxiliary Tests Sbapiro-Wilk's Test indicates normal distribution (p > 0.01) Equality of variance cannot be confirmed Hypothesis Test (1-tail, 0.051 NOEC WEC ChV Steel's Msny-One Rank Test 600 750 670.820393 Treatments vs D-Control TU Statistic Critical Skew Ktut 0.91023314 0.884 0.08328903 1.18006418 Maximum Likelihood-Probit Parameter Value SE 95% Fiducial Umits Control Cbi-Sq Critical P-wlue Mu Sigma Iter Slope 9.94181564 1.30210515 7.3896895 12.4939418 0 0.56435054 7.81472778 0.9 2.92077764 0.10058525 3 Intercept
lO p
-24.037833 3.79565674
IO Q
-31.47732
10 R        s IO T
-16.598346 TSCR Point Probits rna!!: 95% Fiducial Umits ECOI 2.674 486.162395 402.073611 546.054153 EC05 3.355 569.284857 495.375707 621.405179 ECIO 3.718 619.259192 552.924847 666.630381 ECI5 3.964 655.432146 594.893597 699.682767 EC20 4.158 685.682341 629.945281 727.767856 EC25 4.326 712.744582 661.059933 753.440058 EC40 4.747 785.768588 742.116145 827.019952 EC50 5.000 833.254457 791.085439 879.682466 EC60 5.253 883.610019 839.281045 940.16349 EC75 5.674 974.13997 918.466658 1058.63369 EC80 5.842 1012.58695 950.174438 1111.73075 EC85 6.036 1059.32095 987.708178 1177.95731 EC90 6.282 1121.19929 1036.13459 1268.03499 EC95 6.645 1219.62311 1111.01186 1416.02316 EC99 7.326 1428.15045 1263.76751 1745.38103 Organisms obtained from AquaJox, Inc. ppkclcr_II0210 
                                                                                                                                  /D /0 v
\C) <-=:, ..... .ETS * * * * ,_,,,,,,
IO         (Q X
"'
ID 1ilj I                                    1 IO   ca u(       t:t*l\ q*c(         dul         &M       411.(       lo                   a e.~.
Statistical Analyses 1.) Environmental Testing Solutions.
t 14   16.(
Inc. Larval Fish Growth' and Survival Test-7 Da;r Growth Start Date: ll/2/2010 TestiD: PpKCICR SampleiD:
2
End Date: 11!9nOIO LabiD: ETs-Envir.
                                                        ~~      '\ '\                 a'.l     '-'lA     ,  ~  fo--w. cflJJ.           ~..~      5'4         lolA   ~
Testing Sol. Sample Type: Sample Date: Protocol:
I                                    3
FWCHR-EPA-821-R-02-013 Test Species:
                                                              &'J... (\               i
                                                                                                          *~
to
                                                                                                                                ,,~
                                                                                                                                            'i"'-     ~.tl.        510\ s
                                                        '\
                                                                                                        -\,
4                                                                                          f.&,~6.    ~\~                            ~'-\
~I                                                    -,"'"'   &           '\       &         ~            \,.   \o
                                                                                                                                              ,.c,
                                                                                                                                                      '-1.       5 5 "' '-!lit                               l",,       3'U.. ~
5 f         3~          i c.['all                        lo
                                                        "'\
                                                                                        .,vA.       \ .     ~                  '4~
2 I                                    6
                                                        \       R          \,'lA
                                                                                        .,        ~
                                                                                                    .....               ""                  '-            l
                                                                                                                                                        ...~ \St'.
                                                                                                                                                                    \u.    "'L 7
i             (,.                 ~          '1        ~
                                                                                                                                    ~
                                                                                                                                  '"\       '2.       \                 -e-I              A= Pan weight(~
Trayc:olor ~::~
                                                                          \1..'\\ \~-~        \1,..C\~ \?*13                  \1..*"~ \?*~\ \'\.\;              ~9o~ \~:t\
I Analyst:
Date: '\ \.. tn.. \ 0 B- Pan + Larvae weight
                                                      \?rl\
                                                              "*          11. '6?>   \q.~
                                                                                                                  "*""                  l.l).o1 1
1~.1., 1~.>6-t II;,~
l~.r..~ \~.1; (mg)                                                                                                           \~.~t Analyst:          M H::k ll..ltf lb.1o 11.d Date: 1-\. to. lO I              C =Larvae weight (mg)
                =B-A                                  s.os Sf\'-         ~.'\1 $.()'- -a..~              11>-"1 ,..1.1,.      '!t,l.l    '*'-'- \.0'\          O.lo'\    \.i:t I              Weight per Initial nnmber
                                                                                                                                  ..,J.
oflarvae (mg)
                = C I Initial number of larvae o*
                                                        ~
o'-  t;flt.."'
o*        lb*~"' ~
                                                                                      "        o*
                                                                                                'b'Vt, o*
                                                                                                              ~ "3-,'V It)
()'          \:)'      ~*
                                                                                                                                            ~
                                                                                                                                            ~,.
()'
                                                                                                                                                      \~
                                                                                                                                                                  ~
                                                                                                                                                                  ~0..,
0~
I              Average weight per Initial Percent reduction from control  o.5l.<<o                'l..1*1 7.           Q.3'-t\              S'!. *'2.7.         O.l~"Z.                 rL'\1o number of              (%)
I              larvae (mR)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.
I                                                                                                                                      Calculations and data reviewed:       ~
Comments:
Comments:
I 2 3 4 D-Control 0.7090 0.7690 0.8060 0.6250 450 0.8410 0.8460 0.7950 0.9180 600 0.7510 0.7660 0.6170 0.7640 750 0.5050 0.5960 0.4970 0.5060 900 0.3250 0.3470 0.3220 0.3680 1050 0.1660 0.1040 0.0690 0.1880 Transfonn:
I I
Untransfonned Mean N-Mean Mean Min Max CV% N t-Stat D-Control 0.7273 1.0000 0.7273 0.6250 0.8060 10.866 4 450 0.8500 1.1688 0.8500 0.7950 0.9180 5.978 4 -2.541 600 0.7245 0.9962 0.7245 0.6170 0.7660 9.934 4 0.057 750 0.5260 0.7233 0.5260 0.4970 0.5960 8.905 4 900 0.3405 0.4682 0.3405 0.3220 0.3680 6.301 4 1050 0.1318 0.1812 0.1318 0.0690 0.1880 41.676 4 Auxiliary Tests Statistic Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01) 0.91572464 Bartlett's Test indicates equal variances (p = 0.77) 0.51644236 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp Dunnett's Test 600 >600 0.10532876 0.14483158 Treatments vs D-Control Point mit!: SD IC05 542.24 41.38 ICIO 611.14 30.05 ICI5 640.93 22.56 IC20 670.73 19.87 IC25 700.53 18.58 IC40 792.72 16.02 IC50 856.49 11.73 Organisms obtainedfromAquatox, Inc.
IPage 87 of 105 SOP AT2l -Exhibit AT2l.l, revision 04-01-09
469.24 502.48 559.18 606.70 645.14 744.27 818.67 670.91 672.22 696.58 725.29 758.69 831.85 887.00 Linear Interpolation (200 Resamples)
                                                                                                                                                                                    - *"~
Skew 0.3073 -0.6344 -0.6636 -0.4608 0.0590 -0.0054 -0.1586 -*-*] REF-Ref Toxicant KCL-Potassium chloride PP-Pimephales promelas 1-Tailed Isotonic Critical MSD Mean N-Mean 0.7886 1.0000 2.180 0.1053 0.7886 1.0000 2.180 0.1053 0.7245 0.9187 0.5260 0.6670 0.3405 0.4318 0.1318 0.1671 Critical Skew Kurt 0.805 -0.673350488
 
-0.56809922 9.2103405 MSB MSE F-Prob df 0.02055025 0.00466886 0.046436537 2, 9 ppkclcr _II 0210 r, I I I I I I I I I I I I I I I I I I * ----*'= * * * *
_J                                    .-.'-'~-"----'-'~=-=.-.==:.a.:....=..>=-~'-=-="'.>.~=                            -- ..... -
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Pimeohales promelas STOCK , Page 91 of 105 SOP A T21 -Exhibit A T21.1, revision 04-0 1-09 I I I L I " CONTROL ,age 92 of 105 .. _--'*J . '*
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PpKCICR Test Number: ..2.1.0 SOP A T21 -Exhibit A T21.1, revision 04-0 1-09 
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* * * * *fi) Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Envlronme11tal.
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Testing Solution$.
      **                                                                                                Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Metbod 1000.0 r:fY Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
Inc. 1.14 1.12 1.10 1.08 1.06 1.04 1.02 2.5 :::-u 2.0 z 1.5 II) u"' 1.0 * ... 0.5 "CC I t--1.4 1.3 1.2 1.1 1.0 0.9 . . . . USEPA Control Limits (+/- 2 Standard Deviations)  
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Testaomber:                              PpKCICR#IllO Environmental Testing Solutions, Inc.                                                                                                                                                                                    T..t dates:                      November 02-09,2010 I ' "\1~
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c........... (,..tL KO)
****************************************************************
A Iollillo-or 10 J'IMI*amiM:rr*
USEPA Warning and Control Limits (751h and 9dh Percentile CVs) ******************************************************************************************
10 A*Puwtipl (IBI) 13.83 B*fu+Lanot wdPI(IIJI) 20.92 LarYoewdpt(IIJI) Wdgld I S.nlylac
* * * * * . .... * * * * * * . ... * * ...... ... .*.....................................................................*..................
                                                                                                                *A-B 7.09 McuwdPU oolllbcrorlorne(llll) - * * - b e r o r 0.709 Cotllideat or varllllioll
Laboratory Warning and Control Limits (101h and 251h Percentile CVs) ******************************************************************************************
                                                                                                                                                                        <M- .......... _ . . .
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Wdpt/laidllloolllllu orW.oe(llll) 0.709 MeuiRI'ViYal MouweiPUioldll
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(%)      ....ber~Jary-(IIIII
                                                                                                                                                                                                                                                        '.A
                                                                                                                                                                                                                                                          ~or vll'ialloo(%)
                                                                                                                                                                                                                                                                            -*--coatrol(%)
B              10              10              13.75          21.44              7.69              0.769                                                            0.769 Cootrol                                                                                                                                        0.727              10.9                                    100.0          0.727                  10.9          Not applicable c              10              10              13.66          21.72              8.06              0.806                                                            0.806 D              10              10              14.31          20.56              6.25              0.625                                                            0.625 E              10              10              13.75          22.16              8.41              0.841                                                            0.841 F              10                10              14.21          22.67             8.46              0.846                                                            0.846 450                                                                                                                                          0.850              6.0                                      100.0          0.850                  6.0               -16.9 G              10              10              13.58          21.53              7.95              0.795                                                            0.795 H              10              10              14.53          23.71              9.18              0.918                                                            0.918 1              10                9              14.04          21.55              7.51              0.834                                                            0.751 J              10                9              13.44          21.10              7.66              0.851                                                            0.766 600                                                                                                                                          0.805              5.5                                      90.0           0.725                  9.9                0.4 K              10                8              13.69            19.86            6.17              0.771                                                            0.617 L              10              10              14.07          21.71              7.64              0.764                                                            0.764 M              10                7              13.59            18.64            5.05              0.721                                                            0.505 N              10                8              13.77            19.73            5.96              0.745                                                            0.596 750 4.97              0.828 0.154              6.7 0.497 70.0          D.5l6                  8.9               27.7 0              10                6              12.91            17.88 p            10                7              14.40            19.46            5.06              0.723                                                            0.506 Q              10                3              12.94            16.19            3.25              1.083                                                            0.325 I
R              10                4              1323            16.70            3.47              0.868                                                            0.347 900                                                                                                                                          0.919              13.0                                    37.5            D.341                  6.3                53.2 s              10                4              13.79            17.01            322                0.805                                                            0.322 T              10                4              12.64            16.32            3.68              0.920                                                            0.368 v              10                2              13.41            15.07            1.66              0.830                                                            0.166 1050 v              10                 I              14.13            15.17            1.04               1.040 0.875              17.2 0.104 15.0          0.132                  0.0               81.9 w              10                I              14.82            15.51            0.69              0.690                                                            0.069 X              10  -
2              13.71            15.59            1.88              0.940                                                            0.188                                                                        - -*-
Duaett'o MSD value:            0.1053                              MSD=            Minimum Significant Difference PMSD:                          14.5                                PMSD=            Percent Minimum Significant Difference PMSD is a measure of test precisioo. The PMSD is the minimum percent differeru:e between the control and treabnent that can be declared statistically sisnificaot in a whole effiuentwxicity test.
LowerPMSD bmmd detennined by USEPA (lOth percentile) = 12"/o.
Upper PMSD boWld detennined by USEPA (90th percentile)= 30%.
Lower and upper PMSD hoWlds were detennined from the I Otb and 90th percentile, respectively, of PMSD data from EPA's WET lnterlabonllmy Variability Study (USEPA. 200la; USEPA. 2001 b).
USEP A 2001a, 200 I b. Final Report: lnterlabonllmy Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
 
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iETS
                              ; ) Environmental 'JesUng Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 11/212010                      TestiD:      PpKQCR                                  SampleiD:                  REF-RefToxicant End Date:    11/912010                    LabiD:      ETS-Envir. Testing Sol.                 Sample Type:              KCL-Potassium chloride Sample Date:                              Protocol:    FWCHR-EPA-821-R-02-013                  Test Species:              PP-Pimephales promelas Comments:
Conc-ml!!!:        I            2            3            4 D-Control        1.0000        1.0000        1.0000      1.0000 450        1.0000        1.0000        1.0000      1.0000 600        0.9000        0.9000        0.8000      1.0000 750        0.7000        0.8000        0.6000      0.7000 900        0.3000        0.4000        0.4000      0.4000 1050        0.2000        0.1000        0.1000      0.2000 Transform: Arcsin Square Root                        Rank        !-Tailed                Number      Total Conc-ml!i!:    Mean        N-Mean          Mean          Min          Max        CV"Io            N          Sum        Critical                Resp      Number D-Control        1.0000        1.0000      1.4120      1.4120        1.4120        0.000        4                                                      0        40 450        1.0000        1.0000      1.4120      1.4120        1.4120        0.000        4              18.00        10.00                      0        40 600        0.9000        0.9000        1.2543      1.1071        1.4120        9.935        4              12.00        10.00                      4        40
                                      *1so        0.1000        0.1000        o.9939      o.8861        1.1011        9.086        4              10.00        to.oo                    12        40 0 900        0.3750        0.3750        0.6584      0.5796        0.6847        7.979        4              10.00        10.00                    25        40
                                    *1050        0.1500        0.1500        0.3927      0.3218        0.4636      20.862        4              10.00        10.00                    34        40 Auxiliary Tests                                                                                    Statistic                Critical                Skew      Ktut Sbapiro-Wilk's Test indicates normal distribution (p > 0.01)                                      0.91023314                  0.884                0.08328903 1.18006418 Equality of variance cannot be confirmed Hypothesis Test (1-tail, 0.051                NOEC          WEC          ChV          TU Steel's Msny-One Rank Test                      600          750      670.820393 Treatments vs D-Control Maximum Likelihood-Probit Parameter          Value          SE      95% Fiducial Umits                        Control        Cbi-Sq      Critical    P-wlue        Mu        Sigma      Iter Slope        9.94181564 1.30210515 7.3896895 12.4939418                                0        0.56435054 7.81472778          0.9    2.92077764 0.10058525      3 Intercept      -24.037833 3.79565674 -31.47732 -16.598346 TSCR Point            Probits        rna!!:    95% Fiducial Umits ECOI                  2.674  486.162395    402.073611 546.054153 EC05                  3.355  569.284857    495.375707 621.405179 ECIO                  3.718  619.259192    552.924847 666.630381 ECI5                  3.964  655.432146    594.893597 699.682767 EC20                  4.158  685.682341    629.945281 727.767856 EC25                  4.326  712.744582    661.059933 753.440058 EC40                  4.747  785.768588    742.116145 827.019952 EC50                  5.000  833.254457    791.085439 879.682466 EC60                  5.253  883.610019    839.281045 940.16349 EC75                  5.674    974.13997  918.466658 1058.63369 EC80                  5.842  1012.58695    950.174438 1111.73075 EC85                  6.036  1059.32095    987.708178 1177.95731 EC90                  6.282  1121.19929    1036.13459 1268.03499 EC95                  6.645  1219.62311    1111.01186 1416.02316 EC99                  7.326  1428.15045    1263.76751 1745.38103 Organisms obtainedfrom AquaJox, Inc.                                                                                                                                                               ppkclcr_II0210
 
                                                                                                                                                                                                    -*-*]
      .ETS
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      **                                                                            Statistical Analyses
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                                      ,,,~~'
~      1.) Environmental Testing Solutions. Inc.
Larval Fish Growth' and Survival Test-7 Da;r Growth Start Date:      ll/2/2010                    TestiD:      PpKCICR                                    SampleiD:                  REF-Ref Toxicant End Date:        11!9nOIO                      LabiD:        ETs-Envir. Testing Sol.                   Sample Type:              KCL-Potassium chloride Sample Date:                                  Protocol:    FWCHR-EPA-821-R-02-013                    Test Species:              PP-Pimephales promelas Comments:
Conc-m~              I              2              3            4 D-Control           0.7090        0.7690        0.8060      0.6250 450        0.8410        0.8460        0.7950      0.9180 600        0.7510        0.7660        0.6170      0.7640 750        0.5050        0.5960        0.4970      0.5060 900        0.3250        0.3470        0.3220      0.3680 1050        0.1660        0.1040        0.0690      0.1880 Transfonn: Untransfonned                                          1-Tailed                          Isotonic Conc-m~            Mean        N-Mean          Mean          Min          Max            CV%            N          t-Stat      Critical      MSD          Mean          N-Mean D-Control          0.7273        1.0000        0.7273      0.6250          0.8060        10.866      4                                                      0.7886        1.0000 450        0.8500        1.1688        0.8500      0.7950          0.9180          5.978      4            -2.541          2.180      0.1053        0.7886        1.0000 600        0.7245        0.9962        0.7245      0.6170          0.7660          9.934      4              0.057          2.180      0.1053        0.7245        0.9187 750        0.5260        0.7233        0.5260      0.4970        0.5960          8.905      4                                                      0.5260        0.6670 900        0.3405        0.4682        0.3405      0.3220        0.3680          6.301      4                                                      0.3405        0.4318 1050        0.1318        0.1812        0.1318      0.0690          0.1880        41.676      4                                                      0.1318        0.1671 Auxiliary Tests                                                                                          Statistic                  Critical                    Skew            Kurt Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01)                                            0.91572464                    0.805                  -0.673350488    -0.56809922 Bartlett's Test indicates equal variances (p = 0.77)                                                    0.51644236                  9.2103405 Hypothesis Test (1-tail, 0.05)                  NOEC          LOEC            ChV            TU          MSDu        MSDp          MSB          MSE          F-Prob            df Dunnett's Test                                    600        >600                                    0.10532876  0.14483158    0.02055025    0.00466886  0.046436537        2, 9 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point                mit!:          SD              95%CL(Ex~              Skew IC05                  542.24          41.38        469.24      670.91        0.3073 ICIO                  611.14          30.05        502.48      672.22        -0.6344 ICI5                  640.93          22.56        559.18      696.58        -0.6636 IC20                  670.73          19.87        606.70      725.29        -0.4608 IC25                  700.53          18.58        645.14      758.69        0.0590 IC40                  792.72          16.02        744.27      831.85        -0.0054 IC50                  856.49          11.73        818.67      887.00        -0.1586 Organisms obtainedfromAquatox, Inc.                                                                                                                                          ppkclcr_II 0210
 
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I I                          STOCK I                                                        SOP AT21 - Exhibit AT21.1, revision 04-0 1-09
, Page 91 of 105
 
                                                                    .. _--'*J
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I SOP AT21 - Exhibit AT21.1, revision 04-0 1-09
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      ,age 92 of 105
 
      ***                                                                        Ceriodaphnia dubia
      *fi)                                                      Chronic Reference Toxicant Control Chart
    *~      Envlronme11tal. Testing Solution$. Inc.
1.14                          USEPA Control Limits (+/- 2 Standard Deviations) 1.12 1.10 1.08 1.06 1.04 1.02 2.5
:::-                        USEPA Warning and Control Limits (75 1h and 9dh Percentile CVs) u  ~      2.0 z                          ******************************************************************************************
    ~            1.5
    ~
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        ~
1.0
        ~
    "CCI        0.5            .*.....................................................................*..................
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1.4 1          1 Laboratory Warning and Control Limits (10 h and 25 h Percentile CVs) 1.3 1.2 1.1 1.0            -**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**
0.9            ******************************************************************************************
Test date
Test date
* 7-day IC25 = 25% inhibition concentration.
* 7-day IC25   = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. --. Central Tendency (mean IC25) -* -**-Warning Limits (mean IC25 +/- SA.10 or SA.75) * * ** * **** Control Limits (mean IC25 +/- S A15, S A.90, or 2 Standard Deviations)
    - - . Central Tendency (mean IC 25)
Page 93 of 105
    -* -**- Warning Limits (mean IC 25 +/- SA. 10 or SA. 75)
\Q "" ..... Vi * * *
  * *** * **** Control Limits (mean IC25 +/- SA 15 , SA. 90, or 2 Standard Deviations)
* ET Environmental Testing Solutions, Inc. Test number Test date 7-day IC25 CT (giL NaCI) (giL NaCI) I 07-07..00 1.06 2 08-04..00 1.01 1.07 3 08-04-00 1.09 1.01 4 09-15..00 1.05 1.01 5 10-06-00 1.08 1.01 6 10-14..00 1.07 1.07 7 11-10..00 1.01 1.07 8 12-08-00 1.05 1.07 9 01-05-10 1.08 1.01 10 02-02-10 1.01 1.07 II 03-02-10 1.08 1.01 12 04-06-10 1.05 1.07 13 05-04-10 1.09 1.07 14 06-08-10 1.01 1.07 IS 07-13-10 1.01 1.07 16 08-03-10 1.06 1.07 17 09-14-10 1.01 1.07 18 10-05-10 1.08 1.01 19 10-05-10 1.08 1.07 20 11-02-10 1.09 1.07 s 0.00 O.ot O.ot O.ot O.ot 0.01 O.ot O.oi 0.01 0.01 0.01 0.01 O.ot 0.01 O.oi 0.01 O.ot 0.01 0.01 Ceriodaphnia dubia Chronic Reference Toxicant Control Cbart State and USEPA Control Umits SA.Io CT-lS CT+lS 1.06 1.08 0.09 1.05 1.10 0.09 1.04 1.10 0.09 1.04 1.10 0.09 1.05 1.10 0.09 1.05 1.10 0.09 1.04 1.10 0.09 1.05 1.10 0.09 1.05 1.10 0.09 1.05 1.10 0.09 1.05 1.10 0.09 1.05 1.10 0.09 1.05 1.10 0.09 1.05 1.10 0.09 I. OS 1.10 0.09 1.05 1.10 0.09 1.05 1.10 0.09 1.05 1.10 0.09 1.05 1.10 0.09 Laboratory Warning Limits CT-SA.tt CT + SA.Io 0.98 1.15 0.99 1.16 0.98 1.15 0.99 1.16 0.99 1.16 0.99 1.16 0.98 1.15 0.99 1.16 0.99 1.16 0.99 1.16 0.98 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 0.99 1.16 Laboratory SA.lS Control Umits S,\.75 CT-SA-25 CT + SA-25 0.18 0.88 1.25 0.48 0.18 0.89 1.26 0.48 0.18 0.89 1.25 0.48 0.18 0.89 1.25 0.48 0.18 0.89 1.2S 0.48 0.18 0.89 1.2S 0.48 0.18 0.89 1.25 0.48 0.18 0.89 1.25 0.48 0.18 0.89 125 0.48 0.18 0.89 125 0.48 0.18 0.89 1.25 0.48 0.18 0.89 1.25 0.48 0.18 0.89 1.2S 0.48 0.18 0.89 125 0.48 0.18 0.89 1.25 0.48 0.18 0.89 1.25 0.48 0.18 0.89 1.25 0.48 0.18 0.89 1.2S 0.48 0.18 0.89 1.26 0.48 USEPA Warning Umits CT-SA-75 CT + SA-75 0.59 1.55 0.59 1.56 0.59 1.55 0.59 1.5S O.S9 l.SS O.S9 l.SS 0.59 1.55 0.59 1.55 0.59 1.5S 0.59 1.55 0.59 1.55 0.59 1.55 0.59 1.55 0.59 1.55 0.59 1.55 0.59 1.55 0.59 l.S5 0.59 1.55 0.59 1.56 s...,. 0.66 0.67 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.67 Note: 7 -d = 7 -day 2S% inhibition concentration.
Page 93 of 105
An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
 
CT = Central tendency (mean IC,5). S = Standard deviation of the IC25 values. Laboratory Control and Warning Limits USEPA Control Umits CT-S.uo CT + S.uo 0.41 1.73 0.41 1.74 0.41 1.73 0.41 1.74 0.41 1.74 0.41 1.74 0.41 1.73 0.41 1.74 0.41 1.74 0.41 1.74 0.41 1.73 0.41 1.74 0.41 1.74 0.41 1.74 0.41 1.73 0.41 1.73 0.41 1.74 0.41 1.74 0.41 1.74 cv 0.00 0.01 0.01 O.ot 0.01 O.ot O.oi 0.01 0.01 O.oi O.oi 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Laboratory control and warning limits were established using the standard deviation of the IC,. values corresponding to the I Oth and 25th percentile CV s. These ranges are more stringent than the control and warning limits recommended by USEP A for the test method and endpoint SA.Io = Standard deviation corresponding to the I o"' percentile CV. (SA 10 = 0. 08) S.us = Standard deviation corresponding to the 25th percentile CV. (SA.25 = 0.17) USEPA Control and Warning Umits SA-75 = Standard deviation corresponding to the 75th percentile CV. (SA.7s = 0.45) s...,.= Standard deviation corresponding to the 9o"' percentile CV. (SA.90 = 0.62) CV =Coefficient of variation of the IC,s values. USEPA 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Progmm. EPA-833-R-00-003.
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US Environmental Protection Agency, Cincinnati, OH.
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* Precision of Endpoint Measurements
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* Ceriodaphnia dubia
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* Chronic Reference Toxicant Data () Environmental Testing Solutions, Inc. Test Test date Control Control Mean CT cv CT MSD PMSD CT number Survival Reproduction for Control Mean for Control (%) (offspring/female)
      ~
Reproduction
Ceriodaphnia dubia Chronic Reference Toxicant Control Cbart
(%) Reproduction
      ~    Environmental Testing Solutions, Inc.
(%) forPMSD (%) (offspring/female)
State and USEPA                        Laboratory                          Laboratory                                USEPA                                USEPA Test number   Test date     7-day IC25       CT         s        Control Umits        SA.Io        Warning Limits        SA.lS        Control Umits            S,\.75        Warning Umits          s...,.        Control Umits            cv (giL NaCI)   (giL NaCI)             CT-lS CT+lS                      CT- SA.tt    CT + SA.Io            CT- SA-25 CT + SA-25                  CT- SA-75  CT + SA-75              CT- S.uo CT + S.uo I        07-07..00         1.06 2        08-04..00       1.01          1.07       0.00       1.06      1.08       0.09        0.98          1.15    0.18        0.88            1.25      0.48        0.59        1.55      0.66        0.41          1.73      0.00 3        08-04-00        1.09         1.01      O.ot      1.05      1.10       0.09        0.99          1.16     0.18        0.89            1.26      0.48        0.59        1.56      0.67        0.41          1.74      0.01 4        09-15..00        1.05         1.01      O.ot      1.04      1.10        0.09       0.98          1.15    0.18        0.89            1.25      0.48        0.59        1.55      0.66        0.41          1.73      0.01 5        10-06-00          1.08        1.01       O.ot       1.04      1.10        0.09      0.99          1.16    0.18        0.89            1.25      0.48        0.59        1.5S      0.66        0.41          1.74      O.ot 6        10-14..00        1.07        1.07      O.ot      1.05      1.10         0.09       0.99          1.16    0.18        0.89            1.2S      0.48        O.S9        l.SS      0.66        0.41          1.74      0.01 7        11-10..00        1.01        1.07      0.01      1.05     1.10         0.09       0.99          1.16    0.18        0.89            1.2S      0.48        O.S9        l.SS      0.66        0.41          1.74      O.ot 8        12-08-00          1.05         1.07      O.ot      1.04      1.10         0.09       0.98          1.15    0.18        0.89            1.25      0.48        0.59        1.55      0.66        0.41          1.73      O.oi 9        01-05-10          1.08          1.01      O.oi      1.05      1.10        0.09      0.99           1.16     0.18        0.89            1.25      0.48        0.59        1.55      0.66        0.41          1.74      0.01 10        02-02-10          1.01          1.07      0.01      1.05      1.10        0.09      0.99           1.16     0.18        0.89            125        0.48        0.59        1.5S      0.66        0.41          1.74      0.01 II        03-02-10          1.08        1.01      0.01      1.05      1.10        0.09      0.99           1.16     0.18        0.89            125        0.48        0.59        1.55      0.66        0.41          1.74      O.oi 12        04-06-10          1.05          1.07      0.01      1.05      1.10        0.09      0.98          1.16    0.18       0.89           1.25       0.48         0.59        1.55      0.66        0.41          1.73      O.oi 13        05-04-10          1.09          1.07      0.01      1.05      1.10        0.09      0.99          1.16    0.18       0.89           1.25       0.48         0.59        1.55      0.66        0.41          1.74      0.01 14        06-08-10          1.01          1.07      O.ot      1.05      1.10        0.09      0.99          1.16    0.18       0.89           1.2S       0.48         0.59        1.55      0.66        0.41          1.74      0.01 IS        07-13-10          1.01        1.07      0.01      1.05      1.10        0.09        0.99          1.16    0.18        0.89           125        0.48         0.59        1.55      0.66        0.41          1.74      0.01 16        08-03-10          1.06          1.07      O.oi      I.OS      1.10        0.09      0.99          1.16    0.18        0.89            1.25      0.48        0.59         1.55       0.66        0.41          1.73      0.01 17        09-14-10          1.01          1.07      0.01       1.05      1.10        0.09      0.99          1.16    0.18        0.89            1.25      0.48        0.59         1.55       0.66        0.41          1.73      0.01 18        10-05-10          1.08          1.01      O.ot      1.05      1.10        0.09      0.99          1.16    0.18        0.89            1.25      0.48        0.59        l.S5      0.66         0.41          1.74      0.01 19        10-05-10          1.08          1.07      0.01      1.05      1.10        0.09      0.99          1.16    0.18        0.89            1.2S       0.48        0.59        1.55      0.66        0.41          1.74      0.01 20        11-02-10          1.09          1.07      0.01      1.05      1.10        0.09      0.99          1.16    0.18        0.89            1.26      0.48        0.59        1.56      0.67        0.41         1.74       0.01 Note:    7 -d 1~ = 7 -day 2S% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
CV(%) 1 07-07-09 100 29.9 3.3 2.2 7.2 2 08-04-09 100 30.4 30.2 4.7 4.0 2.2 7.3 7.2 3 08-04-09 100 32.0 30.8 5.1 4.4 2.2 6.9 7.1 4 09-15-09 100 31.6 31.0 5.4 4.6 2.2 7.0 7.1 5 10-06-09 100 31.4 31.1 4.3 4.6 2.4 7.6 7.2 6 10-14-09 100 33.3 31.4 3.5 4.4 2.6 7.7 7.3 7 11-10-09 100 34.0 31.8 6.2 4.6 2.6 7.7 7.3 8 12-08-09 100 35.2 32.2 4.6 4.6 2.0 5.8 7.2 9 01-05-10 100 31.1 32.1 5.8 4.8 2.3 7.3 7.2 10 02-02-10 100 31.9 32.1 3.5 4.6 2.6 8.3 7.3 11 03-02-10 100 31.0 32.0 6.3 4.8 2.4 7.7 7.3 12 04-06-10 100 32.7 32.0 6.3 4.9 2.4 7.4 7.3 13 05-04-10 100 31.0 32.0 4.6 4.9 2.3 7.4 7.3 14 06-08-10 100 33.1 32.0 6.6 5.0 2.4 7.2 7.3 15 07-13-10 100 31.6 32.0 4.8 5.0 2.3 7.2 7.3 16 08-03-10 100 28.2 31.8 4.7 5.0 1.8 6.2 7.2 17 09-14-10 100 32.9 31.8 8.7 5.2 3.0 9.2 7.4 18 10-05-10 100 30.2 31.8 7.6 5.3 2.0 6.6 7.3 19 10-05-10 100 30.8 31.7 4.0 5.3 2.2 7.2 7.3 20 11-02-10 100 30.5 31.6 4.9 5.2 2.0 6.6 7.3 Note: CV = Coefficient of variation for control reproduction.
CT = Central tendency (mean IC,5).
Lower CV bound determined by USEPA (lOth percentile)=
S = Standard deviation of the IC25 values.
8.9"/o. Upper CV bound determined by USEP A (90th percentile)
Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC,. values corresponding to the I Oth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint SA.Io = Standard deviation corresponding to the I o"' percentile CV. (SA 10 = 0. 08)
= 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision.
S.us = Standard deviation corresponding to the 25th percentile CV. (SA.25 = 0.17)
The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (lOth percentile)  
USEPA Control and Warning Umits SA-75 = Standard deviation corresponding to the 75th percentile CV. (SA.7s = 0.45) s...,.= Standard deviation corresponding to the 9o"' percentile CV. (SA.90 = 0.62)
= 13%. Upper PMSD bound determined by USEPA (90th percentile)=
CV =Coefficient of variation of the IC,s values.
47%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.
USEPA 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Progmm. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
EPA-833-R-00-003.
 
US Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix.
    **                                                                      Precision of Endpoint Measurements
EPA-821-B-01-004 and EPA-821-B-01-005.
    **                                                                                          Ceriodaphnia dubia Chronic Reference Toxicant Data
US Environmental Protection Agency, Cincinnati, OH. CdNaC/CR_/10210 Page 95 of 105 
()      Environmental Testing Solutions, Inc.
.ET . ' ! ' ...... * ********
Test                  Control      Control Mean Test date                                                CT              cv            CT      MSD      PMSD            CT number                  Survival      Reproduction for Control Mean                  for Control
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements_
(%)       (offspring/female)          Reproduction          (%)    Reproduction            (%)      forPMSD (%)
.::) Environmental Testing Solutions.
(offspring/female)                  CV(%)
Inc. -= 0 = "-"-.: = 1:.1 :! = = ., *c e = =-c.. = -... e = ... = .... = 1:.1 e u ... = cS u -35 40 30 20 10 40 ***** ***********************************************************
1        07-07-09      100              29.9                                      3.3                  2.2        7.2 2        08-04-09      100              30.4                    30.2              4.7          4.0    2.2        7.3            7.2 3        08-04-09      100                32.0                    30.8              5.1          4.4    2.2        6.9            7.1 4        09-15-09      100                31.6                    31.0              5.4          4.6    2.2       7.0            7.1 5        10-06-09     100               31.4                   31.1              4.3          4.6    2.4      7.6            7.2 6        10-14-09     100               33.3                  31.4              3.5           4.4     2.6       7.7            7.3 7        11-10-09     100               34.0                  31.8              6.2          4.6     2.6      7.7            7.3 8        12-08-09     100               35.2                    32.2              4.6          4.6     2.0      5.8            7.2 9      01-05-10      100               31.1                    32.1              5.8          4.8    2.3      7.3            7.2 10      02-02-10       100               31.9                    32.1              3.5          4.6     2.6       8.3            7.3 11      03-02-10      100               31.0                    32.0              6.3          4.8    2.4      7.7            7.3 12      04-06-10       100               32.7                    32.0              6.3          4.9    2.4      7.4            7.3 13      05-04-10       100               31.0                    32.0              4.6          4.9      2.3      7.4            7.3 14      06-08-10       100               33.1                    32.0               6.6          5.0      2.4       7.2            7.3 15      07-13-10       100               31.6                    32.0               4.8          5.0      2.3      7.2            7.3 16      08-03-10       100               28.2                    31.8              4.7          5.0     1.8      6.2           7.2 17      09-14-10       100               32.9                    31.8              8.7          5.2     3.0      9.2           7.4 18      10-05-10       100               30.2                    31.8              7.6         5.3      2.0      6.6            7.3 19      10-05-10       100               30.8                    31.7              4.0          5.3      2.2      7.2           7.3 20      11-02-10       100               30.5                    31.6              4.9          5.2     2.0       6.6            7.3 Note:        CV = Coefficient of variation for control reproduction.
.... *********************************************************************
Lower CV bound determined by USEPA (lOth percentile)= 8.9"/o.
Minimum Acceptance Criteria(>
Upper CV bound determined by USEPA (90th percentile) = 42%
15.0 offspring per surviving female) North Carolina Acceptance Limit ( < 40.0%) Kentucky Acceptance Limit ( < 30.0%) USEPA Upper PMSD Bound (90th percentile
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
< 47 .0%) ";ft. 30 -20 .........
Lower PMSD bound determined by USEPA (lOth percentile) = 13%.
10
Upper PMSD bound determined by USEPA (90th percentile)= 47%.
,1 ,, ,, \fj' \.,. \.,. \.'-' \.'lr ,v ,,. '"'' ,,. \fl' \.' \.' \.\. Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD)
Central Tendency (mean Control Reproduction, CV, or PMSD) Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Page 96 of 105 TS Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species:'
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
Ceriodaohnia dubia CdNaCICR
CdNaC/CR_/10210 Page 95 of 105
#: Comments:
 
water. 1400 21 1479 }.500 < 24-hours old Date and times organisms were born location:
            ..ET    '
Culture board: Daily renewal information:
Ceriodaphnia dubia
Day Date 0 2 3 4 Test initiation and feeding, renewal and feeding, or termination time Acceptance criteria S20%
            .::) Environmental Testing Solutions. Inc.
S20% MHSW batch used 15.0 offspring/female ChV <40.0% IC e.. 1\-Q\* '" Analyst Page 1 of6 SOP A Tl4-Exhibit A Tl4.1, revision 04-01-09 
Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements_
*ers * * . ' ... ****** .*.. . :;, ,..,,....
35                                      ....***** ***********************************************************
., .. _, __ .,.-Emlhn1Mntll1'11tl"g5cllalloN,Ine.
Minimum Acceptance Criteria(> 15.0 offspring per surviving female)
Species:
      -= =
Ceriodanhnia dubia CONTROL Day 1 l 1 Young produced 0 0 Adult mortality
      ~
'-'-l Young produced 0 0 Adult mortality L '--3 Young produced 0 C> Adult mortality
0 40 North Carolina Acceptance Limit (< 40.0%)
\.... '--4 Young produced
:!= .,= 30 1:.1
& Adult mortality L \..... 5 Young produced
      *c e                                                        Kentucky Acceptance Limit (< 30.0%)
,,_ \'1_ Adult mortality
      >~
'-'-6 Young produced D 0 Adult mortality L '-7 Young produced 1"'\ \& Total young produced zt; Final Adult Mortality L-'-X for 3n1 Broods ')(.. ;>(.. Page 2 of6 CdNaCICR
        = =-
#: n!> Survival and Reproduction Data Replicate number ,3 4 5 6 .7 8 9 10 0 () 0 c c 0 _0_ 0 \_.. '--\_.. '--'--'-'-'--D D () a a (\ h Q '-\.... L '-\.... '--c... \.... c 0 0 0 D 0 0 0 '-\.... \_.. \.... L. \.._ L L.... s ''-\ '4 '-\ '-l '-l "'-\ \..... \..,... '-'--'-\._ '--'--LC \A.. 10 10 ,,_ lb \0 \\ '--'-. \...-'-'-\.._... '--'--c 0 *0 0 0 0 0 c '--'-'-L. '-'-'-\..,. \\. \.4$. \c; 1\.o '"' \\ \lc 2.C\ .!>>l. .!>>o '-\.... '-'-L. L. L. '-)(......
c..
_ )Co..._ __ ;<......_
      ...= ...e 20
_lS._ _X _ "1(., _ Note: Adult mortality (L"' hve, D"' dead), SB = spht brood (smgle brood spbt between two days), CO= cany over (offspnng carried over with adult during transfer).
      ....= =
l * . 600 NCIIL mg a s . aJ UTVIV an epro uc 1on a a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced c (:) D 0 0 0 0 0 0 0 Adult mortality L '-'-'-\..... \.... '-L. '-'-2 Young produced 0 c D 0 D c 0 0 0 0 Adult mortality
        ~
'--\...._ '-'-'-L. L '-'-L 3 Young produced 0 0 0 0 0 0 () 0 0 0 Adult mortality L '--'-'-'-'--'-'-'-'-4 Young produced 4 s 'i '-\ t-3 c..l_ -"" '-i ."i Adult mortality
1:.1 e u... 10
'-'-'-l-,\.-'-\.,. L. L '--5 Young produced ll .. lO \1.. n. l't. 10 l\ '\ t\ \\ Adult mortality
        ~
'-'-\...._ \...... '-\._ '-\.... L '-6 Young produced a D 0 c 0 0 0 a 0 0 Adult mortality L '--'--L.. \...._ '-\.._ '-'--'--7 Young produced LS li ,, \ "\ I"'_ tt.. .. '"' l1 Total young produced
u= cS USEPA Upper PMSD Bound (90th percentile < 47 .0%)
:!>>\ o'-\ 3!. "2.., 3\
40
.30 Final Adult Mortality
      ";ft.         30
\. \. ... 'L. \.;... L. \..... '-'-'-\.,.... Note: Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO-cany over (offspnng carried over with adult during transfer).
      ~
emale: SOP AT14-Exhibit AT14.1, revision 04-01-09 Page 98 of 105 II
      ~            20 10 0~~~~~~~~~~~~~~~~~~~~
* i I I I I I I ; I '! ;i lage 99 of I 05 Page 3 of6 Species:
                        ,1 ,, ,,      \fj' \.,. \.,.   \.'-'  \.'lr ,v ,,.   ,~  ~    ,~    ~    '"'' ,,. \fl' \.' \.' \.\.
Ceriodaphnia duhia CdNaCICR
Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
#: \\ 800 N CI/L mg a S . a/ dR d UFVIV an epro uct1on D at a Replicate number Day 1 2 ,J 4 s 6 7 8 9 10 1 Young produced 0 0 c 0 0 c D 0 _C c Adult mortality L. '--"-L.. '-L.. \..._ '-\.._ l Young produced a a 0 c D a a D a 0 Adult mortality
Central Tendency (mean Control Reproduction, CV, or PMSD)
'-L. '--L.. L. '--'-'--'--'-3 Young produced a 0 0 0 C) 0 0 0 0 Adult mortality
    ......... Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
\..._ '-\..-'--"--'-'--'--'-..... 4 Young produced
Page 96 of 105
'-( '-l s '-l "-l s Adult mortality L '-'--'-L... L '--\...._ '-'--5 Young produced lC L'l.. \\. ,,_ lQ IC IC 10 Adult mortality
 
'-\._. \.... '--* L. '---\..._ '-L.. 6 Young produced 0 0 a c '0 0 0 0 0 0 Adult mortality
TS                                                                                                              Page 1 of6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
'--'--'--L.. '--'L-'-L. '-L.. 7 Young produced
Species:' Ceriodaohnia dubia CdNaCICR #:     \\~
''-., .. \.5 \\ l\o \'-\. \'-l Total young produced !O !lo 1.& 1.Ga .!C> '2.&-"l\ Final Adult MortaHb'
\. \ \. \. L.. '-'-'-L \..... Note: Adult mortality (L = bve, D =dead), SB = spht brood (smgle brood spht between two days}, CO= carry over (offipnng carried over with adult during transfer).
Concentration:
% Mortality:
Ql .. Mean Offspring/Female:
l.'l.i % Reduction from Control:
'l..?.t7
.. 1000 mg NCIIL a S . I dR d t" Dat urvtva an epro uc ron a Reolieate number Day 1 l 3 4 5 6 7 8 9 10 1 Young produced
(:) b b 0 _D _() 0 0 0 _Q_ Adult mortality
'-'--\..... \..... L \._. '--\..._ '-L_ l Young produced
_C) 0 D 0 D D D. D 0 Adult mortaUty L L. '-'-\._ '-L.. '-L. L. 3 Young produced 0 0 0 0 0 _0 0 0 0 o* Adult mortality L. \.._ l.... '--\..... L L \._ '-c..... 4 Young produced
'-\ --'i 'i .s* '-( .3 '-i "\ Adult mortality
'-L L L L '-L L. '-L 5 Young produced 10 1\ lC 10 "\ \'l.. IC lC q l\ Adult mortaUty L '--'-L-\..._ L '--L. '-L 6 Young produced 0 0 0 c c 0 c 0 _Q 0 Adult mortality
'-\.._. \..... \...... '-'-'-'-'-7 Young produced l\o '""" \'"\ lb tt-IS Total young produced
'1.t '2..'\ 2.,
'2.b 2.'1 1.'\ Final Adult Mortality L... \..._ '-\. L. '-'-'-\..... \.._ Note: Adult mortality (L = hve, D =dead), SB =split brood (smgle brood spht between two days), CO-carry over (offipnng carried over with adult during transfer).
Concentration:
% Mortality:
0_1. Mean Offspring/Female: % Reduction from Control:
s.1.7. SOP AT14-Exhibit A Tl4.1, revision 04-01-09 I I I I I Page4 of6 Species:
Ceriodaphnia dubia CdNaCICR
#: (l 1200 N CIIL M! a urvm an S 'a/ dR epro uction Data Replicate number Day I 2 tJ 4 5 6 7 8 9 10 1 Young produced 0 _0 0 0 (:} 0 0 0 0 _Q Adult mortality L.. \..... \...... ..__ '--L.. '-'-\...... "-2 Young produced a 0 0 Q D 0 a _C) c c Adult mortality L. '--\.-\.._. '--'--'-L '--3 Young produced
("") 0 c 0 c 0 _0 0 0 Q Adult mortality
\..._ L \.._. '-\..._ L-'-\... \.._. '--4 Young produced
'2.. 0 \ '2.. "" '"' '\... Adult mortality
\...... \.......
'--'---'-\.......
\.......
"-\.... \.......
5 Young produced g 10 la i s , _S_ Adult mortality
'-..__ \.._. \.......
,\.......
'--'-\... L. 6 Young produced 0 _C_ 0 0 0 D _Q 0 C) 0 Adult mortality
'--'--'--'-'-\.._ '--'--'-L.. 7 Young produced
'\ .& 5 , 5 5 .a l.. Total young produced l'l \I\ L1 ''-'\ \\e \\. ,, Final Adult Mortality
'-'--'--'--'-\...... '-'-\..... "-Note: Adult mortality (L = hve, D =dead), SB-spilt brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer).
Concentration:
% Mortality:
: 01. Mean Offspring{female:
% Reduction from Control:
'f1_,q1.
1400 M! NCIIL a S ' I dR urv1va an d t' Dat epro uc zon a Replicate number Dav 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 0 (") 0 0 0 Adult mortality L '--'-\.._ '-'--\,_ '-'--'-2 Young produced 0 D 0 0 a D c 0 0 *a_ Adult mortality
'-'--'--L \... \,... L '-'--'--3 Young produced 0 (!) 0 a _Q 0 0 _Q __Q_ 0 Adult mortality
...._ L. \...... '-L L L '-L L 4 Young produced l 0 '1... 1 0 0 .,_ 0 0 l Adult mortality L-'--L. '-L.. '--\..... '--'--\,_. 5 Young produced c.{ 2. s .s , _3_ --':!_ Adult mortality L '--'--'--'--\,__ b. \_. '--\..-6 Young produced 0 0 0 a a 'L \ \ c lS_ Adult mortality
'-\._ '-L '-'--1 L '--7 Young produced 2 0 0 6 0 0 .... 0 c 2.. Total young produced
, 5 ., "" IC s lo L.\ ., Final Adult Mortality
'--'--'-'-'-_ ...... '-\.... \..... -Note: Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO-carry over (offspnng carried over with adult during transfer).
Concentration:
% Mortalfu':
107. Mean Offspring/Female:
!L""'-LUl.
. % Reduction from Control:
t..ol. tage 100 of 105 SOP AT14-Exhibit AT14.1, revision 04-01-09 
..... ..... v. .ETS . . . * *
* c
.. :*": .. ,:,*, Environmental Tesdng Solutions, Inc. Control Day Replicate number 1 2 3 4 5 6 7 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 3 3 5 4 4 4 4 5 12 12 10 12 10 10 12 6 0 0 0 0 0 Q 0 7 14 18 14 16 15 15 16 Total 29 33 29 32 29 29 32 600 mg NaCI/L Day Replicate number 1 2 3 4 5 6 7 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 4 5 4 4 6 3 4 5 12 10 12 12 12 10 11 6 0 0 0 0 0 0 0 7 15 15 18 17 17 14 16 .___]:'otal 31 30 34 33 35 27 31 800 mg NaCIIL Day Reolicate number 1 2 3 4 5 6 7 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 4 4 3 4 5 4 4 5 9 10 12 11 12 10 10 6 0 0 0 0 0 0 0 7 I6 16 15 13 17 15 16 Total 29 30 30 28 34 29 30 Verification of Ceriodaphnia Reproduction Totals 1000 mg NaCI/L Total 8 9 10 Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 4 4 38 4 4 4 4 5 4 3 3 4 4 3 38 13 10 11 112 I 5 10 II 10 10 9 12 10 10 9 11 102 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 14 17 16 155 7 15 13 16 14 14 17 13 16 16 15 149 30 31 31 305 Total 29 28 30 29 27 32 26 30 29 29 289 1200 mg NaCIIL Total 8 9 10 Day Replieate number Total 1 2 3 4 5 6 7 8 9 10 0 0 0 0 1 0 0 0 0 0 0. 0 0 0 0 0 I 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 42 4 2 3 3 3 1 2 4 2 2 3 25 11 II 11 112 5 8 10 6 8 5 5 7 9 5 8 71 I 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 16 17 15 160 7 9 6 8 5 7 4 5 5 8 6 63 31 32 30 314 Total 19 19 17 16 13 11 16 16 15 17 159 1400 mg NaCI/L Total 8 9 10 Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 5 4 4 41 4 1 0 2 I 0 0 2 0 0 I 7 10 10 13 107 5 4 2 5 5 7 3 4 3 3 4 40 0 0 0 0 6 0 3 0 0 0 2 0 I 0 0 6 14 14 14 150 7 2 0 0 0 3 0 0 0 0 2 7 29 28 31 298 Total 7 5 7 6 10 5 6 4 3 7 60 
.... .... * * *
* e ETS Environmental Testing Solutions, Inc. Concentration (mg/1.. NaCI) 1 2 3 Control 29 33 29 600 31 30 34 800 29 30 30 1000 29 28 30 1200 19 19 17 1400 7 5 7 Dunnett's MSD value: 2.008 PMSD: 6.6 -\-Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR
#113 Test dates: November 02-09, 2010 ,-
'--"I Replicate number Survival Average reproduction Coeflk:ielltof Perceat red*ctioo from 4 5 6 7 8 9 10 (%). (offspring/female) varilllioo
(%) control(%)
32 29 29 32 30 31 31 100 30.5 4.9 Not applicable 33 35 27 31 31 32 30 100 31.4 7.2 -3.0 28 34 29 30 29 28 31 100 29.8 S.9 2.3 29 27 32 26 30 29 29 100 28.9 5.8 5.2 16 13 II 16 16 15 17 100 15.9 15.5 47.9 6 10 5 6 4 3 7 90 6.0 32.4 80.3 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision.
The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10111 percentile)
= 13%. Upper PMSD bound determined by USEPA (9rfl' percentile)=
41%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEP A. 2001 a, 200 I b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix.
EPA-821-B..()
1-004 and EPA-821-B-0 1..005. US Environmental Protection Agency, Cincinnati, OH.
..... ..... **-***--1---
iETS Statistical Analyses Environmental Tesdng Solutions, Inc. Cerioda2hnia Survival and ReJ?!oduction Test-R!:J!roduction Start Date: 11/2/2010 TestiD: CdNaCICR SampleiD:
End Date: 11/9/2010 LabiD: ETS-Envir.
Testing Sol. Sample Type: Sample Date: Protocol:
FWCHR.-EPA-821-R-02-013 Test Species:
Comments:
Comments:
Conc-mg/L 1 2 3 4 5 6 7 8 D-Control 29.000 33.000 29.000 32.000 29.000 29.000 32.000 30.000 600 31.000 30.000 34.000 33.000 35.000 27.000 31.000 31.-000 800 29.000 30.000 30.000 28.000 34.000 29.000 30.000 29.000 1000 29.000 28.000 30.000 29.000 27.000 32.000 26.000 30.000 1200 19.000 19.000 17.000 16.000 13.000 11.000 16.000 16.000 1400 7.000 5.000 7.000 6.000 10.000 5.000 6.000 4.000 Transform:
water.
Untransformed Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat 0-Control 30.500 1.0000 30.500 29.000 33.000 4.948 10 600
1400 21 1479
                                                                      }.500
                                          < 24-hours old                                                    e..
Date and times organisms were born J-;:be~tw~ee=:n=~-:-------r~--:-:--:-:=--:!:-----------1 location:
Culture board:
1\-Q\* '"
Daily renewal information:
Day          Date      Test initiation and feeding,            MHSW        Analyst renewal and feeding, or              batch used termination time 0
2 3
4 Acceptance criteria S20%
                                                                ~80%
S20%
                                                        ~ 15.0 offspring/female    ChV
                                                                <40.0%              IC SOP ATl4- Exhibit ATl4.1, revision 04-01-09
 
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        ~ Emlhn1Mntll1'11tl"g5cllalloN,Ine.
Page 2 of6 Species: Ceriodanhnia dubia                                                                                  CdNaCICR #:        n!>
CONTROL                                                          Survival and Reproduction Data Replicate number Day                              1        l        ,3              4            5          6        .7          8        9        10 1          Young produced      0      0            0            ()        0            c      c            0    _0_      0 Adult mortality    '-        '-            \_..
                                                                                                    '--        \_..
l          Young produced      0        0          D          D              ()        a          a          (\      h c...
Q Adult mortality      L    '-- ' -                  \....        L            '-        \....        '--              \....
3          Young produced Adult mortality 0      C>          c            0            0            0      D          0
                                                                                                                                              \.._
0      0
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4          Young produced      &      ~            s\..... ''-\              '4        '-\        '-l        ~        '-l      "'-\
5 Adult mortality Young produced      ,,_L
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                                                                            \


==Dear Mr. Patrick Cromer:==
==Dear Mr. Patrick Cromer:==
SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR DECEMBER 2010 Enclosed is the December 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 12/18/2010 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.
 
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete.
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR DECEMBER 2010 Enclosed is the December 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant.
I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 12/18/2010 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
Sincerely, k gs Site Vice Presid t Sequoyah Nuclear Plant Enclosure cc (Enclosure):
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc: B. E. Brickhouse, LP 5U-C D. A Day, POB 2B-SQN S. A Howard, OPS 5N-SQN K. Langdon, POB 2B-SQN D. B. Nida, LP 5U-C U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, DC 20555 A A Ray, WT 11A-K G. R. Signer, WT 6A-K M. D. Skaggs, OPS 4A-SQN B. A Wetzel, OPS 4A-SQN K. M. Hodges (EDMS), LP 2V-C 1 I 1 &#xa3; l Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 January 1 0, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement  
Sincerely, n~            k gs Site Vice Presid t Sequoyah Nuclear Plant Enclosure cc (Enclosure):
& Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534  
Chattanooga Environmental Field Office                 U.S. Nuclear Regulatory Commission Division of Water Pollution Control                     ATTN: Document Control Desk State Office Building, Suite 550                       Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc:
B. E. Brickhouse, LP 5U-C                               A A Ray, WT 11A-K D. A Day, POB 2B-SQN                                   G. R. Signer, WT 6A-K S. A Howard, OPS 5N-SQN                                M. D. Skaggs, OPS 4A-SQN K. Langdon, POB 2B-SQN                                  B. A Wetzel, OPS 4A-SQN D. B. Nida, LP 5U-C                                    K. M. Hodges (EDMS), LP 2V-C
 
I 1 l
1&#xa3; Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 January 10, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534


==Dear Mr. Patrick Cromer:==
==Dear Mr. Patrick Cromer:==
SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR DECEMBER 2010 Enclosed is the December 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 12/18/2010 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.
 
Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete.
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR DECEMBER 2010 Enclosed is the December 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant.
I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 12/18/2010 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
Sincerely, Michael D. SkJg Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) AdQ[ess _p ....Q.JtOX 2000 ___________ _
Sincerely, r~~~
______ _
Michael D. SkJg Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
___ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMR) I --
Chattanooga Environmental Field Office                            U.S. Nuclear Regulatory Commission Division of Water Pollution Control                                Attn: Document Control Desk State Office Building, Suite 550                                  Washington, DC 20555
J I I I I YEAR MO MQ PAY From 10 \12 01 To !10 12 I 31 MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT
*** NO DISCHARGE D ... Form Approved. OMB No 2040-0004 NOTE: Read instructions before completin!l this form PARAMETER X QUANTrrY OR LOADING OUALrrY

Latest revision as of 12:58, 1 March 2020

SQN Annual Water Withdrawal Updates for July 2010
ML13289A198
Person / Time
Site: Sequoyah  Tennessee Valley Authority icon.png
Issue date: 08/11/2010
From: Church C
Tennessee Valley Authority
To: Cromer P
Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
Shared Package
ML13289A109 List: ... further results
References
Download: ML13289A198 (204)


Text

{{#Wiki_filter:S58 100811 800- NPDES CORRESPONDENCE August 11, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6"' Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JULY 2010 Enclosed is the July 201 o Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: B. E. Brickhouse, LP 5U-C A A. Ray, WT 11A-K C. R. Church, OPS 4A-SQN G. R. Signer, WT 6A-K S. A. Howard, OPS 5N-SQN B. A Wetzel, OPS 4A-SQN K. Langdon, POB 2B-SQN G. J. Wynn, POB 2B-SQN D. B. Nida, LP 5U-C K. M. Hodges (EDMS), LP 2V-C

Tennessee Valley Authority. P::*st (Jj1ce Sex 2CDO. Scddt Oaisf', Tenressee 3138.:!-2-JCC August 11,2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6'* Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JULY 2010 Enclosed is the July 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, 4_t;Uz Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission A TIN: Document Control Desk Washington, D.C. 20555

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT July DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Bethany Hyatte DATE: 8/5/2010 CONCURRENCES Name R c Signature - Comment Date v N S. A. Howard X _4twheutM._

                                        ,_ ..,.:.--     (\ .iJo!IDOA   ~           S')CJ llo G.J. Wynn                      X     ' \. \r)..)....F*                             ~h\\c B. A. Wetzel                   X       ~'1.* J

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                                     '-                      c.;-

INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

PERMmEE NAME/ADDRESS (Include Facilitv Name/Location if Oifferenl) NATIONAl POllUTANT DISCHARGE EliMINATION SYSTEM (NPDES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (OMR) Jllame_ _ Til_A~ S_EOUOYAH_I'JIJCL_E~R ~~N!_ _ _ _ (SUBR 01) OMB No. 2040-0004 _A_ddfe~~ ___ P_,Q.__Il_OX2_QOO_ __ _ _ _ _______ _ c~~~~~~B~R DISCHARG~~~M~ER 1 _____ttNJE...RQEOCU6:28_*SQNl _ _ _ _ _ _ _ _ F- FINAL _ _ _ _ SQD!LY _cDI\[S_'L lli >>~- ______ _ 11 DIFFUSER DISCHARGE

£9Qjjt;y_ _ .TYA_:_S.EQUQ'ffiH_l.ll,K:L.f.l\KP.!._A_~T _______ _

t~TING ro[D~~t ~ J.Q@!Q_ _H_8.M!!:um_..cOY~TY____________ _ EFFLUENT I YEAR MO MO QA [ l *** ATTNo stepnanie A. Howard From 10 I 07 01 10 07 I 31 *** NO DISCHARGE NOTE: Read instructions before campletin~ this. form.

                                                                                                                                                                                                                  --------~-----.----

PARAMETER QUANTITY OR LOADING OUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE UNITS AV~I<Aljt: UNITS I - ANAlYSIS

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EFFLUENT GROSS VALUE n '>' DAILY MX PERMIT TEMP. DIFF, BETWEEN SAMP. & SAMPLE ******** I ******** ~ 1,0 04 0 I 31 I 31 I CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 EFFLUENTGRoss_vALUE s 0 PERMIT REQUIREMENT

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           ~E-PRINCIPAL           EXECUTIVE OFFICER ICertilyunderpen<~ltyofl<~wthOII*t.hisdocum. entandallattadlmentswereprepa.,... undt!rmy                                                          ~~-

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                                                                                                                                                                                           /                                            TELEPHONE                      DATE
                  ****-*------------- ---*-*--* ------~~- direction or superv1s1on in accord<ince wtth a system des~gned to assure that qualified personnel                           /    ~                                                                           -

01~ com~lete. ~are sub~it ing -~-..-t-Christopher R. Church properly gather and evaluate the Information submitted. Ba5ed on my 1nqu1ry of the person or ,.,.:; person5 who manage the system, or those persons directly responsibl&ior gathenng the Sequoyah Site ice President

                             .    *         .               inlormallon, the Information Sl.lbmltled IS, to the best of my knowledge and belief, true,                                                                                        843*7001         10        08      09 uoyah S1te. VIce Prestdent                accurate,                  I am        that there are significant penalties for              false           SIGNATURE OF PRINCIPAL EXECUTIVE                                    ______
                  ----- -
  • information, 1nclud1ng the poss1b111ty affine and 1mpnsonmentfor knowing v1olat1ons. OFFICER OR AUTHORIZED AGENT NUMBER YEAR M UAY T!_~~~-9R PR~-~~ED ___ _ __

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (1/eference all attachments her No closed mode operation. The following information is included in attachments: CCW data, veliger monitoring data, and thermal discussion.

 - - - - - - -*            ~cc=c---~-=--c---o--

Page 1 of 2 EPA Form 3320-1 (REV 3199) Previous editions may be used

DMR Attachment CCWData CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained. CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 07/14/2010@ 1042 <0.10 mg/1 07/15/2010@ 2125 JOB EPH

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Temp. ("C) Sample Date Asiatic LOCATION LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp. ("C) Clamslm3 Clam 11/03/2009 133 0 16 11/03/2009 76 16 lnplant RCW CMW 11/10/2009 417 6.1 16 11/10/2009 25 16 lnplant RCW CMW 11/17/2009 269 0 16 11/17/2009 0 16 lnplant RCW CMW 11/24/2009 36 50 15 11/24/2009 18 15 lnplant RCW CMW 12/01/2009 32 0 13.5 12/01/2009 0 13.5 lnplant RCW WE 12/08/2009 38 0 11 12/08/2009 0 11 lnplant RCW CMW 01/05/2010 0 0 6 01/05/2010 0 6 lnplant RCW B 01/12/2010 0 0 5 01/12/2010 0 5 lnplant RCW 01/19/2010 0 0 6 01/19/2010 0 6 lnplant RCW p 01/26/2010 32 0 7.5 01/26/2009 0 7.5 lnplant RCW NRT 02/02/2010 0 0 7 02/02/2010 0 7 lnplant RCW MSW/WDT 02/09/2010 0 0 8 02/09/2010 0 8 lnplant RCW BUTC 02/16/2010 0 0 5 02/16/2010 0 5 In plant RCW BJ 02/23/2010 11.7 0 7 02/23/2010 0 7 In plant RCW BJ 03/02/2010 0 0 6 03/02/2010 0 6 In plant RCW PB 03/09/2010 0 0 8 03/09/2010 0 8 In plant RCW MJW 03/16/2010 0 0 10 03/16/2010 0 10 In plant RCW BC 03/23/2010 14 0 11 03/23/2010 0 11 In plant RCW BC 03/30/2010 0 0 14 03/30/2010 0 14 In plant RCW BAPO Apr-2010 no samples collected May-2010 no samples collected Jun-2010 no samples collected Jul-2010 no samples collected

For ten days in July, the daily maximum 24-hour average temperature river temperature was above 30.5 C. In all instances, Sequoyah complied with State of Tennessee NPDES Permit No. TN0026450 (effective date September 1, 200S), Part 1, A. Effluent Limitations and Monitoring Requirements for Outfa\1101, page 2 of 25 bullet 5 which states ,"The daily maximum 24-hour average river temperature is limited to 30.5 C. Since the state's criteria makes exception for ~xceeding the value as a result of natural conditions, where the 24-hour average ambient temperature exceeds 29.4 C and the plant is operated in helper mode (full operation of one cooling tower, at least three lift pumps, per operating unit) the maximum temperature may exceed 30.S C. In no case shall the plant discharge cause the 1-hour average river temperature at the downstream edge of the mixing zone to exceed 33.9 C without the consent of the permitting authority." The below table is provided as a demonstration of when Sequoyah met these requirements of the permit during the month of July. 24-hour 24-hour 24-hour Maximum 1 Maximum 1 Number of average average average Hr average Hr average Cooling downstream ambient ambient Downstream Downstream Tower Lift temperature temperature temperature temperature temperature Pumps in July {Celsius) {Fahrenheit) {Celsius) {Fahrenheit) {Celsius) Service

                                            >29.4 deg C                       < 33.9 deg C 9            30.8              87.5            31.1             88.1             31.2               6 10            30.6              87.1            30.6             87.4             30.8               6 16            30.7              87.2            30.4             87.8             31.0               6 24            31.2              88.1            31.4             88.7             31.5               6 25            31.2              88.2            31.3             88.0             31.1               6 26            30.7              87.3            31.0             88.1             31.2               6 27            30.7              87.2            30.8             87.1             30.6               6 29            30.8              87.4            31.1             88.0             31.1               6 30            31.1              87.9            31.8             89.5             31.9               6 31            31.3              88.3            32.1             88.7             31.5               6

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approveel. DISCHARGE MONITORING REPORT (DMRJ

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                                                                                                                                                                                                     *** NO DISCHARGE          D ...

ATTN: Stephanie A. Howard 50060 0 0 EFFLUENT GROSS VALUE TEMPERATURE- C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT

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EXECUTIVE QFFICE:"i~ d1rect1on PRINCIPAL ---------------- Christopher R. Church 1-Cert1fy .under pen.ally of1nlaw or superviSIOn that.this d~ment accotdance an*d*. all with a system ~ttachments designed P.repar. we." that to assure ed under qualified properly gather and evaluate the infonnalion submitted. Based on my inqu1ry of the person or my personnel z; r.;z;.~* TELEPHONE *-- --,-----DATE 1--,....::__ :_-=-. --~

                                                                                                                                                                                                                                                                           --~

Sequoyah S1te Vtce Prestdent persons who manage the system, or !hose persons directly responsible for gathering the linformalion, \he information submitted 1s. to the best of my knowledge and belief, true, aa::ISate, and complete. 1am aware that there are s1gnific.:ant penalties for subrnltttng talse information, 1nclud1ng the possibility of flne and impr1sonment for knowing Vtolallons. Sequoyah Site Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGE NT 423 AREA t 1 843-7001 10

                                                                                                                                                                                                                                   --N._U_M_B_E_R-----1-YEAR --

08 MO-- 09 DAY IL._ _ _ _.:.TY.:.:.PED OR_Pil."":r*o. *- I ~~;;; - .-- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attacnments ner The following injections occurred: 1. Floguard MS6236 (max. calc. cone. was 0.062mg/L-Iimit 0.2mg/L) 2. Biodetergent 73551 (max. calc. cone. was 0.017mg/L--Iimit 2.0mg/L) 3. Spectrus CT1300 (max. calc. cone. was 0.039mg/L-Iimit 0.050mg/L) 4. Spectrus CT1300 (low detection level analytical method was <0.050mg/L-Iimit 0.050mgll) EPA Fonn 3320-1 (REv .)l"lml Previous editiOit>> may J.X: u>>c-u Page 2 of 2

PERMITIEE NAME/ADDRESS (fr?Ciude Facilitv Name/location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SVSTEM (NPDES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMRJ Rame__ TlfA_: $1;~9_Y~H_Nl)CLEAR ~U\N!, ___ _ (SUBR 01) OMB No. 2040-0004 MQr~ ___P...Q ...§PX200Q __ ~---- - * - ----- CJ!~~~~!R II rnscHARO~ ~~M:ER I - _ ~ _I!N_TEEQEFJCU~&.S.Q_Nl_ _ _ _ _ _ _ _ _ F- FINAL ______ SQ.PQY .:..P.8.!SY. INM~.-- _ _ _ - --- _ BIOMONITORING FOR OUTFALL 101 E~QLmL. __TYl\_:_SECYQ"LBtfll!J.CLEARJ~J.A.NJ _.. -* _ _ _ EFFLUENT t~TING~t .!:fK~ti.Q_ _H_A_M!!,T.QNSQ!.!fiD'_____ .. _ _ _ _ _ _ _ To \i 07 AITN: stephanie A. Howard From I YEAR 10 I MO 07 01 MO I DA)' 31 I ~* NO DISCHARGE D ... NOTE: Read instructions before completinq this form. PARAMETER ---:Qc:Uc:AN=T=rr=oV:-:O::R:-L:-:0:-:A.~D::IN::C:;:------,----------o:==c-:c~=====c-- *---------*-,- ~----*- . ---

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                                                                                                                                                                                                                                                                              ~-*-J;;J***

Christopher R. Church properly gather and evaluate the Information subm*tted. Based on my 1nqu1ry of the person or persons who manage u-.e system, or those persoos d*raclly respoos1ble for gathenng the Sequoyah Site ice p tdent

                          .   .         .                mformat*on, the information submitted IS , to the best of my knowledge and bel~ef. true,
  • 423 843-7001 10 08 09
                                                        ~~ccurate, and complete. I am aware that there~ Significant penalties fDf slilmitting false                                                                                                               *--***-~---

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 ~                                                       tniOfmatlon, 1nc1ud.ng the poss1btllly affine and tmpnsonmentlor knowing v*otat*ons.                                     OFFICER OR AUTHORIZED AGENT                               AREA           NUMBER             YEAR      MO      DAY

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PERMITTEE NAMEJADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ Form Approved MAJOR DISCHARGE MONITORING REPORT (DMR) N.a!ll_e__TIJA- S_E~~'f!!.H_l'll)CI,.EAR Ptj\N!_ __ _ (SUBR 01) OMB No. 204o-0004 &ldress ___P_.Q._J!.OX2.ooo__ __ --** _ _ _ _ _ _ _ _ _ I p~~~~!~~ B~R ~~M:ER I 5

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rNiiME/TITlE PRINCIPAl EXECUTIVE OFFICER f--"-"." -*-- -------------- --- "- - --- Christopher R. Church f*;;,fy ""'"' peo*tyof tow th,. th;, documam ;"" *_II a_nachffients.were prepared ur1dermy d*recllon or supervision Jn acoorda.-.::e wllh a system des1gned to assure lhat qualified properly gather and evaluate the Information submitted_ Based on my *nqutry of the peiSOfl or personnel

                                                                                                                                                                                             ~                      'f.t#                          ;---2ElEPHONE-                      -- - -

petsons who manage the system,_or thoSe persons d1reclly responsible for gathenng the Sequoyah Site Vice President Information, the *nlormat*on submitted 1s . to the best of my ktlowledge and belief, true, 423 843-7001 10 08 09 Sequoyah Site Vice President I ~~~tNUMBER accwate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE

~                       _____                 ----iinform..ahon, 1nclud1ng the possibility of fine arxl imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT YEAR MO_- DAY L_____,_ TYPED OR PRINTED _L *- - - - - - - - - - - - COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments net PCBs were sampled on 6/30/2010. EPA Form 3320*1 (REV 3199} Previous editions may be used Page 1 of 1

PERMIITEE NAME/ADDRESS (Include FaciJitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMRJ Jl!ilme_ T\fA_: ~Eql)QY~H_N~CLE:Ail ~~NT__ (SUBR 01) OMB No. 2040-0004 A_d!l[e_g~ __p....Q.Jl.OX2QOQ_ _ __ __ __ _ _ _ _ _ _ _ 5 L_P;R%~~~~: B~R JIDISCHARG:~~M~ER I _ _ _ _(!N_TfBQEF:K:UIU&S.QNL _ _ _ _ _ _ _ _ F- FINAL _ _ _ ___SQPQY..:..PAls.'L lli-..1?3B.4_____ _ LOW VOL. WASTE TREATMENT POND F_~Q!l~ _nfl\..:..SEOUO'i.Ati !\1 U1=LE_A_R_ elAJ'!T_ __:_ __ _ t~RING ~~t 1QQW2_ _H.AM!rJ:Q_N_CQ!Ji"'T'f_ _ _ _ _ _ _ _ _ _ _ _ EFFLUENT I YEAR I D ... ATTN: Stephanie A_ Howard From 10 I MP 07i( ro[ 10 MP 07 I pey 31

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qccordance ~ot-and Wllh a system all. *_.ttachments. designed ""_"that to 8S$Ure ' .__'_'_'_d qualified under my personnel ~ ~&-" / _A'(/'? TELEPHONE- DATE--~

                                                                                                                                                                                                                                                                                                          -- -- --     f~

Christopher R. Church p.-operly gather and evaluate the Information submilted. Based on my mquny of the person or persons who manage the system. or those persons directly responsible lor gather1ng the Sequoya Site ice President

                             .        .        .               informo:~tion, the information subm1tled 1s, to the best of my knowledge and belief, true,                                                                                              ---=~~-I 423             843~7001            10      06            09 Sequoyah Site Vtce President                         accu-ate, and complele. I am aware that there are sign*f*can\ penalt~es_for subn11tt1ng false                                  SIGNATURE OF PRINCIPAL EXECUTIVE                                                     -*-~- -*-              -~- ___ _

_ - - - - * - ------- -** 1nformat1on. 1nclu~11ng the P<>Sstblllly of f1ne and 1mpnsonmenl for koow1ng violations OFFICER OR AUTHORIZED AGE NT AREA MO DAY 0 LC::-0::-M-M---=E::-N=TS=-.A::~~:~::~~~~ 0F ANY VIOLATIONS tl?eFerence all attachments hel ---- __co_ --- ------*- --- . *- -- EPA Form 3320~1 (REV 3199) Previous editions may be used Page 1 of

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NP0£8) MAJOR Form Approved

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                                     -*-*---------j'nlormallon, including the p05sibility of fine and impnsonment ICA" knowing violations.                                                                                                                                             *-

OFFICER OR AUTHORIZED AGE NT --+1-vE_A_JR NUMHtK TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments het No Discharge this Period EPA Form 3320~1 {REV 3199) Previous editions may be used Page 1 of

PERMITTEE NAME/ADDRESS (Include Facility NameA..ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) f-l~me .. _ TyA_:_ S.EO.lJI:)Y~H~ll£1£~R ~~!'!!_ __ _ (SUBR01) OMB No. 2040-0004 Adate.M_P..Q.Ji@_2QOO___ ____________ _ c:R%~~~~-J r ~~M~ER _______t1NJ!iR'QEFJC~SIU8:SQ_N_L _ _ _ _ _ _ _ F -FINAL

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                                 .      .              .                     1nformatton, the 1nf00T1ai1on submitted is , to the best of my knowledge and belief, true,                                                                                                                                                                              423            843-7001 Sequoyah S1te VIce President                                     accurate, and complete. I am aware that there~ Significant P~:~nalbes_tor submitting 1a1se                                                                                                          SIGNATURE OF PRINCIPAL EXECUTIVE                                 f--::~*- -*~*- ______ --*- --*

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PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAl POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR .r;_ame__TlfA~ SEQUOYAH_NIJCLE;AB PIJ>.NT ____ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) M~r~~S __ P~--~_OX2.Q9fL ~ __________ _ c~~!~: e~a PISCHARO;~~:t:J _____ J!t!T~~QfFJr;U~8.:.~QNL _____ - - _ _ 5 F- FINAL ______S.QDQY __:_ PA!~Y_,_ TN --37~4- _ __________ _ 1 RECYCLED COOLING WATER Eildll~. __TY_A_:__S~YQY.A~!J.CLE_A_R_e!AMJ _ _ _ _ _ 1 1 p:!~ 1Q~t[Q_ _H_A_MJ!, TQN.__CQUNIY_ _ _ _ _ _ _ _ _ _ _ _ EFFLUENT YEAR MO r:qRING MQ o*x NO DISCHARGE [X)(j ' " ATTN: Stephanie A. Howard From 1 10 1 07 01 To 10 07 1 31 1 ex NOTE: Read instructions before completinQ this form

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert~ under penalty of law lhat this doo:;:lMllent and all auactunellls ware prepared ~der my sk.'s~~nt TELEPHONE

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                    -*---*                            direction OF sup80'ision in accordance wil.il a sys.tem designed lo aSSl.lfe lhal ql!BiifJed pe!Sormal Christopher R Church                    properly gather and evaluate the llll'onnalion subrnltted. Basad on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the information, the information subm*1ted !S, to the best of my knowledge and belief, true,                                                                             423     843-7001           1 Sequoyah Site Vice President I--AREA-~---NUMBER-accurale, and complete I am <~ware th<lt there are significant penalties fOf submitting false                    SIGNATURE OF PRINCIPAL EXECUTIVE mformat1on. including the possibility of fine and 1mpnsonment !Of knowing violations                                                                                                         --
-----                                                                                                                                                                      OFFICER OR AUTHORIZED AGENT                                             V!

TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS tReference all attachments her No Discharge this Period EPA Fonn 3320-1 (REV 3199) Prevrous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Fonn Approved. !'Lame~- --~A_:_ S_~ql)OYAH NlJCLI:~ ~~1\1!_ ___ _ DISCHARGE MONITORING REPORT (OMRJ (SUBR 01) OMB No. 2040--0004 P.--Pilre__£?_P..Q._6_Q.lt2_QDt;L ____________ _ ______IINTI;BQEFJCU~B.:.S~l_ _________ _ ______SQDID .:..P.8!5_Y_,_ U~ .J.n6A____ - - __ Eaci!Jt;v__1Yl:\_:_5f.OUOYAt:L~_lEJJ;.A_R_&_AHT__ [ P;R~~~~ B~R 5 JI PISCHARQ: ~~!:] F- FINAL BACKWASH EFFLUENT

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                                                                                                                                                                                                        ~-~-----                  ~-               ~--

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1Certify under penalty d law that ttus document and all attachmeols were prepared under my DATE

                                                                                                                                                                                                                                      .tt; d1rect1on or supervtsioo in accordance with a system designed to assure that qualified personnel Christopher R Church                      properly gather and evaluate lhe mlonnat1on submitted_ Based on my inquiry of lha person or persons who manage the system, or lhasa persons direclly responsible for galhenng the Sequoyah Site Vice President n1formatJon, the informatiOn subrrutted 1s , to the best of my knowledge and belief. ttue.

Sequoyah Site Vice President accurate, and complete. I am aware that there are significanl penalties lor submitting false SIGNATURE OF PRINCIPAL EXECUTIVE -~--- i11format1on, 1nclud1ng the possibility of 11ne and imprisonment tor knowing VIOlations.

          ------*                                                                                                                                                   OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPlANATION OF ANY VIOLATIONS                         tReference all attachments her Operations performs visual inspections for floating debris and oil and grease during all backwashes.

EPA Form 3320--1 (REV 3/99) Previous editions may be used Page 1 of 1

(Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ Form Approved. PERMITIEE NAME/ADDRESS MAJOR DISCHARGE MONITORING REPORT (DMRJ Name__~A_:: SEQ_UQ_Y~H_I'IUCLEAjl ~~N!_ __ _ (SUBR 01) OMB No. 2040-0004 1\.~ru:e~__pjj. E!PX..2.Q90__ -~-~-~ ____ ~* __ _ _ _ -* _llf..!Je!?:QE8.Ci;;.SIU8:.S.Q..NL -* ~*- _ _ _ _ _ __SilllQY :Pi\!S.'L I!\! 2~-- _______ _ .E9QUt.Y... _ T\11\_:_SE9UO"ffiti.!\I!JC~A..R.~.1ANJ.~ _ _ _ _

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EFFlUENT YEAR MQ MQ DAY *** NO OISCHARGE CJ ... ATIN: stephanie A_ Howara

         -----PARAMETER                       -  X ~/                            AVERAGE From 10 OUANTITY OR LOADING MAXIMUM                        UNITS 01           To 10 MINIMUM OUALITY OR CONCENTRATION AVERAGE NOTE: Read instructions before completinQ this form MAXIMUM
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l --It -___L_~ _j___~~ Christopher R. Church REQUIREMENT * .. *** 1Cert1ty under penally of law thai this oocument and all attachments were prepared under my d*rect1on or superviSIOn in accordance With a system designed to ass. ure that quali.fied personnel properly gather and !Valuate lhe 1rfonnat1on submrtted. Based on my *nqu1ry of the persan or persons_who manage the system,. or tnose persons d1rectly respons1ble for gat_henng the I_ * *. I u l£4 *  ;.-* Sequoyah Site Vice President TELEPHONE 1------- --

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Information, the informal* on subm1tted IS , to tlle best of my knowledge and belief, true, 423 843* 7001 10 OS 09 Sequoyah Site Vice Pcesident ~:ccurate, and compiEIIe. I em aware that \here are s1gnif1cant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I ~ _ ___ __

                         --cc-----::=c-----              n1ormat1on. *nclud~ng the possibility of fine and impnsonment for knowing v1oJat1ons.                                         OFFICER OR AUTHORIZED AGENT                         AREA        NUMBER         YEAR     MO     DAY TYPED OR PRINTED                                                                                                                                                                                                        ..COOf             *---     __        __

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PERMITTEE NAME/ADDRESS (Include Facili('{ Name/Location if DiffererrtJ NATIONAl POLLUTANT DJSCHARGE ELJMJNATJON SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR)

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              - PRINCIPAL EXECUTIVE 0.**----     FFJCER      1 ~ertify dnect1on    or superv1$10n under   penalty of1nlaw that. this w1th ment accordance               and. all a system      il:llachment!>.~.

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docu. Christopher R. Church prop.my gather and eva!uatelhsirdcnmat!onsubmit!ad. Basei! on my1nqwry of !he person or persons who manage the system, or those persons directly responsible for gathenng the equoyah Site ice President

                            .     .         .                1nforma!ion, the informahon submitted IS, to ttle best of my knowledge and belief, true,                                                                                                423           843-7001             10        08       09 Sequoyah Stte V1ce President                      accU""ate, and comPlete. 1am aware that there ~e s~nificant penalties fDf subm1ttmg false                                 SIGNATURE OF PRINCIPAL EXECUTIVE                                      I f-----           _-*- -..~                                 1nformat1on, 1nclud1ng the poss1b111ty of fine and 1mpnsonment for knOWing v1olat1011s                                       OFFICER OR AUTHORIZED AGENT                               AREA~           NUMBER -- YEAR                MO      DAY TYPED OR PRINTED             ____                                                                                                                                                                                                 Cllllf. .

LC_O_M_M_E_N-TS_A:_::_NO."-'EXPLANATION OF ANY VIOLATIONS (Reference all attachments he! - - --- -------- - -- -- --- During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320*1 (REV 3/99) Previous editions may be used Page 1 of 1

S58 100909 800- NPDES CORRESPONDENCE IV September 9, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6'" Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR AUGUST 2010 Enclosed is the August 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. On August 21 and 23, 2010, verbal notifications were made for the Towerbrom 960 release that occurred on August 20, 2010. This was followed by a written submission on August 24, 2010. A copy of the written submission is attached. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments weffl prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely ~!& Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C 20555 cc: B. E. Brickhouse, LP 5U-C A. A. Ray, WT 11A-K C. R. Church, OPS 4A-SQN G. R. Signer, WT 6A-K S. A Howard, OPS 5N-SON B. A Wetzel, OPS 4A-SQN K. Langdon, POB 2B-SON G. J. Wynn, POB 2B-SQN D. B. Nida, LP SU-C K. M. Hodges (EDMS), LP 2V-C

Tennessee Valley Authority, Post Ot'1ce Box 2*:00. Sodd'J SlaiS'J. Tannessee 3732.!-2000 September 9, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1 6 " Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR AUGUST 2010 Enclosed is the August 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. On August 21 and 23,2010, verbal notifications were made for the Towerbrom 960 release that occurred on August 20, 2010. This was followed by a written submission on August 24, 2010. A copy of the written submission is attached. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sin~y, / C---~lf{q Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk

  • Washington, D.C. 20555

PERMITIEE NAME/ADDRESS (Include Facility Name/LocatiOn if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved MAJOR DISCHARGE MONITORING REPORT (DMR) ffilm_e_ _TVA_:_ ~~ovJIH!"I!CL!!AR 1'1*~-~ __ _ (SUBR 01) OMB No. 2040-0004 Mctre_g;_p_,_g_JlQX2.Q91L ~ ~--- ________ _ 5 I p;~~~~~:s~R J IDISCHARG:~~M~ER I _(IN_TfBQEFj!;;UiiU!l§QNL _ _ _ _ .. _ _ F- FINAL _ _ _ _ SQQID' .o.Pi\!SL IN ~>!i4_ . _ _ _ _ .* _ DIFFUSER DISCHARGE fi<:Jtily_ _T)/jl. ~--SEOYQYf!H_NJJC!.fAR.etAJ'iT _ _ _ _ _ .bQ~;;;:~_t[Q_ _H_ti_Mlbl__Q_N...CQ!Jfil.Y____________ _ ATTN: stephanie A. Howard From 10 I I YEAR MO r:qRING 08 01 To m!l I 10 MO 08 I 31 I PAX EFFLUENT

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uous _......==--. .c J;'~j NAME/TITlE PRINCIPAl EXECUTIVE OFFICER I CeJ1ily under penalty of taw that ttln~ doosnent al)d all attachments were prepared under my \ 7 .?*7 7 l DATE

                - - - - - - - *- - - - - - * * - -              direction or- supervision 1n accordance With a sySiem designed to assure that qualified pe.-sonnel Christopher R. Church                        properly gather and evaluate the information submitted. Based on my inquiry or the person or persons who manage !he system, ar thOse persorn; directly resP<Ntslbl& for galnenng lh8 information. the informahon submilled is , to the bast of my knoWledge and belief, true, Sequoyah Site Vice         President              accurate, and complete. I am aware that !hare are sigmriCSilt penalties_ for submitting false                SIGNATURE OF PRINCIPAL EXECUTIVE

~- ~--~~ mformat1on, 1nclud1ng the pOSSibility of fine and 1mpnsonment for knowmg violations. OFFICER OR AUTHORIZED AGENT L___ TYPED OR PRINTED cc~o~M~M~EN~r=s~ANDEXPlANAil-oN_O_F__ A_N_YV~IO~LA~T~IO=N~S~-~R-~-~-re--n-ce--~~~~---c-n-m-e-n-~~n-et________________________.J______________________________~~~ No dosed mode operation. The following information is included in attachments: CCW data, veliger monitoring data, and thermal discussion. EPA Form 3320-1 (REV 3/99) PreviOus editions may be used Page 1 of 2

DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained. CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 08/04/2010@ 1035 <0.10 mg/1 08/06/201 0 @ 11 04 RJS EPH

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Temp. ('C) Sample Date Asiatic LOCATION LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp. ('C) Clams/m3 Clam 11/03/2009 133 0 16 11/03/2009 76 16 lnplant RCW CMW 11/10/2009 417 6.1 16 11/10/2009 25 16 lnplant RCW CMW 11/17/2009 269 0 16 11/1712009 0 16 In plant RCW CMW 11/24/2009 36 50 15 11/24/2009 18 15 lnplant RCW CMW 12/01/2009 32 0 13.5 12/01/2009 0 13.5 lnplant RCW WE . 12/08/2009 38 0 11 12/08/2009 0 11 lnplant RCW CMW 01/05/2010 0 0 6 01/05/2010 0 6 lnplant RCW B 01/12/2010 0 0 5 01/12/2010 0 5 In plant RCW 01/19/2010 0 0 6 01/19/2010 0 6 lnplant RCW p 01/26/2010 32 0 7.5 01/26/2009 0 7.5 In plant RCW NRT 02/02/2010 0 0 7 02/02/2010 0 7 In plant RCW MSWIWDT 02109/2010 0 0 8 02109/2010 0 8 Inplant RCW BLITC 02/16/2010 0 0 5 02/16/2010 0 5 lnplant RCW BJ 02/23/2010 11.7 0 7 02/23/2010 0 7 In plant RCW BJ 03/02/2010 0 0 6 03/02/2010 0 6 lnplant RCW PB 03/09/2010 0 0 8 03/09/2010 0 8 lnplant RCW MJW 03/16/2010 0 0 10 03/1612010 0 10 lnplant RCW BC 03/23/2010 14 0 11 03/23/2010 0 11 lnplant RCW BC 03/30/2010 0 0 14 03/30/2010 0 14 In plant RCW BAPO Apr-2010 no samples collected May-2010 no samples collected Jun-2010 no samples collected Jul-2010 no samples collected Aug-2010 no samples collected

For two days in August, the daily maximum 24-hour average temperature river temperature was above 30.5 C. In both instances, Sequoyah complied with State ofTennessee NPDES Permit No. TN0026450 (effective date September 1, 2005), Part 1, A. Effluent limitations and Monitoring Requirements for Outfall101, page 2 of 25 bullet 5 which states ,"The daily maximum 24-hour average river temperature is limited to 30.5 C. Since the state's criteria makes exception for exceeding the value as a result of natural conditions, where the 24-hour average ambient temperature exceeds 29.4 C and the plant is operated in helper mode (full operation of one cooling tower, at least three lift pumps, per operating unit) the maximum temperature may exceed 30.5 C. In no case shall the plant discharge cause the 1-hour average river temperature at the downstream edge of the mixing zone to exceed 33.9 C without the consent of the permitting authority." The below table is provided as a demonstration of when Sequoyah met these requirements of the permit during the month of August. 24-hour Number average of downstream 24-hour 24-hour Maximum 1 Maximum 1 Cooling temperature average average Hr average Hr average Tower (Celsius) ambient ambient Downstream Downstream Lift temperature temperature temperature temperature Pumps in August (Fahrenheit) (Celsius) (Fahrenheit) (Celsius) Service

                                                >29.4 dee: C                    < 33.9 dee: C 1           30.8              87.8              31.0             86.7            30.4               6 14           30.6              87.1              30.6             87.5            30.8               6

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NAME/TIT_!£ PRINC~~_AL EXECUT_!Y_~OFFICER 1 Certify under penally of law that lh1s document and all attachments were prepared under my TELEPHONE DATE direct/Of! or supe!YISiOT! m acc.ofdance witll a system designea to i!ISSufe ttlat quatifie<l personnel --~"- -*-

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Christopher R. Church property gather arnl evaluate the *mrormabon 5l.lbmit1ed. Basad on my Inquiry of lhe person or pSfsUls who manage th& system, or those pe~rsons directly responsible for gathenng the Information, the information $Libmltled is , to the best of my knOWledge aocl belle!, true, 423 843-7a01 Sequoyah Site Vice President accurattt, aocl complete. I am aware that there are sigmlicant penalli~ for submitting false SIClNAT\JRE OF PRINCIPAl EXECUTIVE information, Including the pos~,;ibility of f1ne and lffiPflsonmenl for knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER TYPED OR PRINTED ---------- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (J?eterence all attachments he! EPA Form J32v--1 tru:v .JI:I:If Previous ediliOI~:> mdy 1Jt:! uoot'u Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facility NameA...ocation if DifferentJ NATIONAl POllUTANT DISCHARGE ELIMINATION SYSTEM (NP0£SJ Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMRJ ttarrte_ _ Tli_A_~ ~OEI>_Y ~H_!jlJI:~A/1 ~NT_ __ _ OMB No. 2040-0004 r-p:~~~;~:e~a rDJ:ARG:~~:ER (SUBR 01) A..d!1fe~s ___ P_._Q.JtO_X ZOOQ_ _ ~- ___ *~ ____ _ _____U.N.TEBQEI:!~UfH&:_S_Q__NL _ ~ _______ _ F -FINAL ___ -~QIIQY cPA!SL m 2~4- _______ _ 1 BIOMONITORING FOR OUTFALL 101

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                                                 ~---                                                                                                                                                                                                     '""~" *~-~. ~-DAY Christopher R. Church                    properly gather and evalu_ ate the 1nformatron Sllbmrtted. Based on ~Y inqurry of the person or                                         .r      {: (                    -~

persons who manage !he system, or those persons ctrredly responsrbre tor gatherrng the Sequoyah Site Vice resident

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  • 423 I 843-7001 10 09 07 Sequoyah Site V1ce President accurate, and complete. I am aware ll"lst ltlere ere sigrufiomt f1$f!8/IJ&s_ tar submrtlrng fals8 SIGNATURE OF PRINCIPAl EXECUTIVE -*- _ _ . ___ _

[ _____ .. nlformatron, rncludirrg lhe possibdrly of frnu and rmprrsorvnent lor knowing 'o'IOiat1ons OFFICER OR AUTHORIZED AGENT AREA f - _"!'VPED OR PRINTED mnF ------- _ COMMENTS AND EXPlANATION OF ANY VIOlATIONS /Reference all attachments het Toxicity was not sampled in August 2010. EPA Form 3320-1 (REV 3/99) Previous editions may be usee:! Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved !'Lame_ _ TlfA ~SEqtJO_Y J\_H_NUC(.E~ !'IJIN"f_ __ _ DISCHARGE MONITORING REPORT (OMR) OMS No_ 2040-0004 (SUBR01) p._!jm-f!s_s J,Q.__epx 2_QpQ_ _ -* _ _ _ _ _ _ _ -~ _ I p:~~~!~~~ER J IDISCHARO~~~M~ER I _____<tNTERQEr:Jl;U~*78cSQI"L _ _ _ __ _ F- FINAL __ ___ _ _ S.Q.PJLV_:_Dl\JSY_. lli ..17~4~--- ________ _ LOW VOL WASTE TREATMENT POND .EflQlity_. _TYl\JEOUOY!:\H_!~.Illtl.f.A_R_ eL_ANJ_____ _ EFFLUENT t~RINO D~~t J.Qg_t!Q_ __1-U\M_IU_QNJ;;QYN__TY____________ _ From 10 I 08 IYEAR MO o1 To~ 10 08 I 31 I MO QAY

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I I 1---- L_ _ _ _L_ ___t__ _ _ J___ _ ~ NAME/TITLE PRINCIPAL EXECUTIVE OFFICE:R r Cerlily under penalty Of law that ttl1s document and all attachments. w.ere pr. epared under ...7 d!rechon or sup&JYJSJOO 111 accordance WJih a system designed to assure that qualified personnel

                                                                                                                                                                                                                                        ---TELEPHONE--

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                                                                                                                                                                                                                                                             ------r-Christopher R. Church                  prop6rly gather and evaluate the Information submllted. Based on my Jnqutry of the person or persoos Who manage the system, or those persons direct~ responstble for QBthEinng the Sequoyah Site Vice    President inrormatton, the information submitted is , to the best at my knowledge ana belief, lrue,                                                                                          423     ' 843-7001     I  1 o accurate, and complete. I am 61ware that there are signtficant penalties for submttting false                      SIGNATURE OF PRIN\..l...-AL                            IIVt:          I TYPED OR PRINTED
                                   =----**--!informal ton, tncluding the possibiltly of hne and imprisonment for knowing vtolattons OFFICER OR AUTHORIZED AGENT t:At\..U AREA CODE I   NUMBER         YEAR COMMENTS AND EXPLANATION OF ANY VIOLATIONS                       ll?eference all attachments her EPA Form 3320--1-(*R-EV--31-99_)____-;:p:-,.-,-::lo'u*-.cs-=e-cd,;;"tic-on"s=-m=--cay-b:.-e:c::us:ced:-:;----------------------------------------- - - - -                                                                                                   Page 1 of 1

PERMITTEE NAMEJADDRESS (lnclud~ Facility Name/Location if Different) NATIONAl POllUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Farm Approved. MAJOR DISCHARGE MONITORING REPORT IOMR) !<.11!!1e_ _Tif"~ SE{J~jiH_II~L,EAR I"U\_N'f __ _ OMB No_ 2040-0004 1H1!U~~___p.Q_J!OX2_QQQ_ ______ ~ ______ ~ _ _ _ _ _II!'J_T!;!<QEFZUij:Z85lt_NL _ _ _ ~- __ _ _ _ _ _ SQ!JDY ~1\!S'L 11l ~?~4- _ _ _ _ _ _ _ b -----\h TN0026450 PERMIT NUMBERDISCHAROE NUMBER

                                                                                                                                                                                                            *~107 G I

(SUBR 01) F- FINAL METAL CLEANING WASTE POND ~Qlifx_ _:r_VA_:_S&QIJQYAiiNYCLEA.RI?.bAlff_ _ _ _ _ 1Q~t[Q_ ~HAM_ll.1_Q_f\l£QIJI'f[Y_ _ -~ ATTN: Stephanie A. Howard From 1 10 YEAR I osoT MD tirNGri To io I MD 08 PAY 31 1 EFFLUENT

                                                                                                                                                                                                                                           ... NO DISCHARGE                 @] ...

NOTE: Read instructions before complet;nq this form. r---- --PARAMETER-- OUANTfTY OR LOADING QUALITY OR CONCENTRATION No. -lFRE0UfN-CYJ SAM.PLE EX OF ) TYPE AVERAGE MAXIMUM MINIMUM AVERAGE MAXIMUM ANAtYSIS I=---~- ---- - ---~ PH ~PLE

                                                                                                                                                           ~

MEASUREMENT 12 00400 1 0 0 PERMIT .... ,_&'-o 'iJ*'.-~::;.-_' -__<<-,u<<-..'***-.-~-: 9.0 su DAILY GRAB EFFLUENT GROSS VALUE REQUIREMENT MINIMUM"' : .* /

~U~IU~, ----*
             -------~--
                         ----~-**-

I u I AL SU5i=i:.NLiELi SAMPlE MEASUREMENT MAXIM~M 19 MGIL DAILY fcoiXPOS

                                                                                                                                                        ....                                                                                                                       1---- I I .* 4~*t]~;:~;r~;~**-                                             *~t ~AI~; MX_

00556 1 0 0 PERMIT **"""**'~~'* DAILY-tG-RAs REQUIREMENT

                                                                                                                              **'****'**                                                                                                                                  MG/L EFFLUENT GROSS VALUE PHOSPHORUS, TOTAl. (AS P)                                     SAMPlE                          ********                        ********                                      ********                                   ********
                                                                                              ......... r . . . ..                                                                                                                                                         19
                                                                                                                                                           ~

MEASUREMENT 00665 1 0 0 PERMIT ., .... 1*, , ~"'***'fl'**--<'j(; ~-'iS'i*' *********- -.-Nc-,--. *;*{() MGIL DAILY \COMPOS EFFLUENT GROSS VALUE REQUIREMENT

                                                                                                                                                                       * *j.;"           !'f~**;YJ**                     ** * * ** .ir.:          DAILY Mx COPPER, TOTAL (AS CU)                                         SAMPLE                          ******"'*                       ********                     ~

MEASUREMENT 19 01042 1 EFFLUENT GROSS VALUE

--~-------

0 0 PERMIT REQUIREMENT

                                                                                              **-"'~~11****
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MG/L I DA~ y lCOMPOS IRON, TOTAL (AS FE) SAMPLE ******** ******** "'******* ******** MEASUREMENT

                                                                                                                                                           ~

19 01045 o a PERMIT REQUIREMENT fll*flllllil**"'* ***"'**** ~ .. ~- "'*****-~ n.<17 ***~~*:*~;:_:: . ~J{-:1-': :-~:r'::~1 ~i* MGIL l-----i---oA1LYfcoMPOS EFFLUENT GROSS VALUE ':_'[ _:_'---iiiD£1)jJLYMX

*tuw, IN TREATMENT PLANT
                 ~CONDUIT OR THRU                             SAMPLE MEASUREMENT                                                                                     03 I ....~-~~ t"l : ........ :,........                                                            ~ ~r-DAILy--tCALCTD I

50050 1 0 0 PERMIT REPORT REPORT MGD EFFLUENT GROSS VALUE

-~ -----             ~----

REQUIREMENT MOAVG DAILYMX 1 - --l' '"-'('-' .,*:::;* *~-~--: }" .-.. ,. -~r _j_ ______ ________L_____. _____j M.-E/Trfl.E

          - - - - - -PR!NCIP
                     - - ---~..-Al EXECU.
                                      ~ ---~-----

0

                                           -TIV*E* . FFJCER direciKJn
1. Certify ."""*. , pe. nalty of10taw .. all we<. ethat p<epa.. ".d under my TELEPHONE -1~ MU  :-~

t or supervision that .this document

                                                                                                   <lCCOTdallC$               and W!lh a system      attachments.

desiQned to assure qual1h&d pel"$0flnel --- -- - :.[  : Christopher R. Church properly gather and evaluate lhEt mformalion subm1tted. Based on my Jnqu1ry of the person or pefSO(IS who manage the sy~9m, or those~ d!leclly responSible for gathanng the

                           .       .
  • JnformatJon, the tnlorrnatJon submitted is, to the best of my knowledge and belief, true, 423 843-7001 10 09 07 Sequoyah S1te Vtce Prestdent ao;;u-ate, aM complete 1am aware that !here are SJ9nificant penallies for submittJ~ false SIGNATVRE OF PRINCIPAl EXECUTIVE _ _ __ _ __

__ _ _ * ~ ____ ~ information, mcludtng the posslbtltty ol fine and impnsonment fDr knowtng violations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY _____ TYPED OR PRINTE_!?____ _ ___ _ COMMENTS AND EXPLANATION OF ANY VIOlATIONS /ReFerence all attachments her No Discharge this Period

--*--*~=

EPA Form 3320-1 (REV 3199) Prevrous editions may be used --* ----;;age 1 oi-1------

PERMITTEE NAME/ADDRESS (Include Facility Name!LocaDon if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved MAJOR DISCHARGE MONITORING REPORT (DMRI Rame_ _T\I_A_:_ SEO~~~I'jl)C~A_R I'_~N!_ __ _ (SUBR 01) OMB No. 2040-0004 1\_ddt:e~ _P_,_O.Ji01t2.QOO_ ~ _ _ _ _ _ _ _ _ _ _ _ I p~R~~~~~:B~R J ~~M~ER I _ _ _ _ ll~JfRQfEK:E Sf!:~t'...:S.Q_Nl_ **- _ ,_ _ _ _ _ F- FINAL _ _ _ _SQPQY _,j)l\!SJC.lli 2~ __ - - __ -- [DISCHARG: RECYCLED COOLING WATER faQlity_ _TYA_0[QI1QY8t-!11U{:(gAR.eb_AN_T_____ _

                                                                                                                      ~                      t~TfNG p~t

~Q!tiq __H_A_MJJ,TQ.N__cQYN..IY_ _ _ -* EFFLUENT I

                                                                                                                      \0 I                                  To~ 10                                                                            lXIJ ***

R MO MO QAY

                                                                                                                                                                                                           -* NO DISCHARGE ATTN: stephanie A. Howard                                                                                From                          08       01                               08 \ 31 NOTE: Read instructions before completinQ this form
------                                  -                                                                                                                                                                                                              -- --- ----,-=---El
                                               ~

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS I

                    ---------                                                                                                                                                                                                                                    !-----       ..1 - - - -

TEMPERATURE, WATER DEG. ***"'**** ******111* ******** ***"'**** MEASUREMENT 04 04 CENTIGRADE 00010 z INSTREAM MONITORING 0 0 PERMIT REQUIREMENT *-****** . I* *.- DEGC . ~.....**..... . . I. *- _*. . /J

                                                                                                                                                                        /~'*'
,~t~-*- ; 1'  : ..38.3 If DAILYMX DEGC r--- -----

DAILY

                                                                                                                                                                                                                                                                                -~---

GRAB-4 PH SAMPLE MEASUREMENT 12 00400 1 0 0 PERMIT ***"***" ******"'* **** (&;()~ *;, '" ,,*"'*~.~*-*:.:*:*. )_( I (*9.0 su WEEKLY GRAB REQUIREMENT 2 EFFLUENT GROSS VALUE MINIMUM'" .;;. "; t . 1 MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 19

                                                                                                                                                                                                                                                       - -ro-AILv 'CcJMPOS
                                                                                                                                                    --***~ r*.'~i;,.~:*.-.- ,;"y*I*~

00530 1 0 0 PERMIT ****""*** ' " ' "******* ... ' . "*_ 30 . . MG!l EFFLUENT GROSS VALUE REQUIREMENT -.*.  ; *._*. . ..*.. _ ... i'i>DAILYMX OIL AND GREASE SAMPLE MEASUREMENT 19 v:x*~;~;;~;f~ ~ ,,fi;.t:. :;;

  • DAILY MX C----. ~~--

00556 1 0 0 PERMIT REQUIREMENT-

                                                                                                                 . *-       ..                                                                                .-.-.* i_-15                   MG/L                     DAILY        GRAB EFFLUENT GROSS VALUE
             --~--

FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT 03

                                                                                                                                                                                                                                                                                  --~--
                                                                                                                                                                                                              "T*'.: . . ~~*..*****           *h*
.,_....;.)' '*i.

i ,. ,_. . -,*,; ~;,_;1r: . 50050 1 0 0 PERMIT REPORT .**REPORT MGD DAILY CALCTD EFFLUENT GROSS VALUE REQUIREMENT MOAVC I .OAILYMX_ . .; .* .. CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT I., .. :"* ******** _..- ******** *'~~****11:* 19

                                                                                                                                                                                                                                                                                 ~--
                                                                                                                                                            ... ~..:;*,-~*

50060 1 0 0 PERMIT MG/L CWEEKLY GRAB-4

                                                                                                                                                                                ,{                    *r .\j'0.10 REQUIREMENT                                                                                             ."

EFFLUENT GROSS VALUE  :-DAILYMX_ SAMPLE

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MEASUREMENT PERMIT .. **

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                                                                            ..                    I
  • li-.- * -lOi ctl*"lJo:-.lii"
                                                                                                                                                                                                                                             """"" r --J L__ _ _ _ _ _ - - - ' - - - - - -
                ..~-~--*~~*

Christopher R. Church

                                                -~Q,r.,.dlon or suoervtslon 1n accordance w1th a system des,gned to assure lhal qllB.IIf1ed pe~nel properly gathe'r and evaluale the intOITflalion suDmrlled. Based on my inq~.~iry of ih~ Vl:lnoon or
                                                                                                                                                                                                                                        ---        ---            -    ---r,-~-M.

b persons who manage the system, or those persons direct~ responsible for gathenng the _ . . . *nformat!on, the *nforiTIQtton submitted is, lo the best of my knOw/edge 8(<<j belief, true, 423 843~7001 10 09 07 Sequoyah S1te V1ce Preslden_t accura1e. and complet&. 1am aware thai there are sign*ficant penal has for subm1tt1ng false SIGNATURE OF PRINCIPAL EXECUTIVE ___ _ _ __ _ _

      . ____                  _ _ _ _ __             mtom1atron, rocluding llle posSJblllty of tme and 1mpr1SD11menl fcx knowing viOlalwns.                                   OFFICER OR AUTHORIZED AGENT                                           NUMBER              YEAR MD        DAY TYPED OR PRINTED COMMENTSAN_D__E_X_P_LA_NA-T-IO_N_O___F~A~N~Y~V~IO~LAc~T~IO=oN~Sc--IR=e-~c---n-c-e-acnca-a2C~-hcn>e---n-tsc-hec-r-----------------------------"-----------------------------------"~na""---------

No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS {lncNlde Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved DISCHARGE MONITORING REPORT (DMRJ _'l;lmL _TifA_:_ ~~OY~H~~loEA~ ~~N!_ __ _ (SUBR 01) OMB No. 2040-0004 &tctrf:l.~ _ .-~ _p_l>. IIDX.2_@Q_ _ -~- __________ _ _ _UJ:!l~RQfFJCE.SIUA*SQN!.. ________ _ ____ -~ SP-'lll.Y _o_P8!SYc I!'1 2:![4_ _ _ _ _ _ _ _ FTN0026450- l I PERMIT NUMBER ) DISCHARGE NU 110~ F- FINAL RECYCLED COOLING WATER .f.iiQ!ity_ _TVf\..:...SEQYQ'LI:\H_I\IUC!.E_AKPJJ\liT_____ _

                                                                                                                    ~                      t~TING p~t I I

!-..Qcat[Q__HA_M_U,TQN..CQ!.!I'fi.Y_____ ~- ______ _ EFFLUENT ATTN: stepnanie A. Howard From 0o I AR MQ 06 01 Tor 10 MQ 08 QAY 31

                                                                                                                                                                                                       *** NO DISCHARGE                    ~ ***

NOTE: Read instructions before completin!:l this form. ,------~ PARAMETER I

                                                 ~

OUANTrrY OR LOADING OUALrrY OR CONCENTRATION - - - - - NO:** FREaliENCY'SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANAlYSIS

                   -------~-~~---
                                                                                                                                                                                                                                                     - - r---        ~-
                                                                                                                                                                                                                                                                        ;----  ~--

IC25 SJATRE 7DAY CHR ******** ******** ~ CERIODAPHNIA MEASUREMENT 23 '

                                                                                                                                                                                                                                                  ;-----~

TRP3B 1 0 0 PERMIT ******** ******** *~* 45'2.': L:,:()__*~****. "' ;_: *'*****flo* PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE

 ~------------*

MINIMUM "

                                                                                                                                                                                                                                        ~-

ANNUAL

                                                                                                                                                                                                                                                            ~-- c-~--

IC25 STATRE 7DAY CHR SAMPLE ******** ******** ~ MEASUREMENT 23 PIMEPHALES TRP6C 1 0 0 PERMIT REQUIREMENT

                                                                                   ******""'                 *********                   ~ ..               .45.2.~;;. ,..., -:-'*_~~****** :_:_--:!:f;,: k',_-- >*'****Ill:**
                                                                                                                                                                                    ." -,__. <./

PERCENT

                                                                                                                                                                                                                                                  ,------     SEMI       COMPOS EFFLUENT GROSS VALUE                                                                                              .                                    MIN!MONi'                         '_

I_ : ANNUAL SAMPLE MEASUREMENT

                                                                                                                                                    - ~* ;~~*;~1 ~f. ~c3 r }i'*~)~~ ~r**.

PERMIT

             ~---~---*

REQUIREMENT. I' .. .:."' .. - SAMPlE MEASUREMENT

                                                                                                                ..*                                                                                                                               ;----                   *---~

PERMIT ~ . .*. REQUIREMENT  : I tC,J.i,~ I .:[?;*:, **. :*:j )~*. u'1?;******* . .-*--- SAMPLE MEASUREMENT r--- "*-*--~ f----- PERMT' REQUIREMENT SAMPLE

                                                                                          *~*

I

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                    ------~

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                                                                                                                                                                                                                                   ~-

1[:1 I --- NA_ME/TITLE E_~IN~IP~L EXECUTIYE OFFICER NAME/TITLE I Cert1fy under penalty of law that thiS document and all attachments w6fe prepared under my ' --7 TELEPHONE-d1rect1Qrl or suoerv1s10n 1n accordance With a system designed to 9$sure that qualified personnel DATE .

                                                                                                                                                                             ~.4                     ~
     ~----

cChnstopher R Church ~P"'P~ly galh" and.,~"'" lhe 'nfonnat*on *ubm*tlod """' oo my '"""'"' 011.. pe<*on oc persons v.tlo manage the system or thOl>e persons dtrecUy responsrble lor gathermg the Sequoya~ ~ice P r e s i d e n t r l i t o Sequo Sequoyah S1te V1ce President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS rnlonnatton, the rnformatron st.bmrtted tS to ttu.1 best of my knowledge ana belief, true accl.-fate, anQ complete I am aware that there are stgnrficant penalt~es for submrttrng false rnformabon, rncludmg the posstbrhty of frne and lffipnsonment 101' knoWing vrolattons (Reference all attachments her SIGNATURE OF PRINCIPAL EXECUTIVE OFFtC-~R OR AUTHO~ED AGENT ~= _ 843 _70 1 NUM~ER __ Y 10

                                                                                                                                                                                                                                                                 ~~~

071 No Discharge this Period

        ~o=~~=c--                        ---c~~-=----c---,----------------------------------;c;-                                                                                                                                                              --;-;-c-~*---

EPA Form 3320~1 {REV 3199) Previous editions may be used Page 1 of

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ Form Approved. MAJOR J'l_ame___TVA~ ~Q_lJOYII_H_!'IIJ_CLEA~ ~LAN!_ __ _ DISCHARGE MONITORING REPORT /DMR! OMB No. 2040-0004 (SUBR 01) Ad~!8M _.P.J)._MQX_~ --*- _________ - - - - - - - P;R~~~;~!;B~R \[  !~M~ER \ ____ <LI'HfiBQEFJC~J!IU&SJlNL _ _ _ _ _ _ _ F -FINAL 1 _____SQOQY..: ...0.8ISL TN 2?:N4__ ________ ~ , DlSCHARO; BACKWASH _Bic;:Ult:'L _T}U\--=-.5E9U9Y.8li.NUCbE.A.RelAN_T___, __ _ 19catLQ. _H_8NIJ!,:I:Qr-u:;QU__ ATTN: Stephanie A. HOward From 10 I YEAR I 08 pzqRJNCMO 01 To 10 rsmt 08MO I~ j1 EFFLUENT

                                                                                                                                                                                                               *** NO DISCHARGE                  [ ] ***

NOTE: Read instructions before comoletinq this form

                                                                                                                                                                                                                                            ----        No~    fiEO-ueNCYS-AMPLEl A

PARAMETER QUANTITY OR LOADING OUAUTY OR CONCENTRATION EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS: FLOATING(SEVERITY) SAMPLE MEASUREMENT

                                                                                                                                                -                    ********                    **-****                        0                9A       0      1131 *VISUAL --*

01345 1 EFFLUENT GROSS VALUE 0 0 PERMIT REQUIREMENT

                                                                                                                                               -**                  ******~***"'
                                                                                                                                                                         ...... ****'. 1'~*--****--***.iii
                                                                                                                                                                                                                  -. ._REPORT
                                                                                                                                                                                                                 .;MOTOTAL PAS&-0 FAIL:1 SEE PERMIT VISUAL OIL AND GREASE VISUAL                                       SAMPLE                      *******"'                        0                                            ********                    *****"'**               *******"                         0       1/31       VISUAL MEASUREMENT                                                                              94                                                                                               **
                                                                                                                                                                    ...........                                                                                    sEE rViSUAl-I~:~* f **0~**" ,'* ..

84066 1 1-* "/ uu 0 0 PERMIT ******** REPORT YES*1 *****"'** REQUIREMENT. NO=O *j;*: EFFLUENT GROSS VALUE MOTOTAL *_ 1 1

  • PERMIT 1----------*- - - - - - -

SAMPLE MEASUREMENT PERMIT

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PERMIT REQUIREMENT . I .< . **- ** ... <.*ffi I I**. __:_  :.::__ - SAMPLE MEASUREMENT r---- L~ 1 '.: "::._Z:(1!71 PERMIT . .

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REQUIREMENT I~ 1 *** 1*c1, ,** **...:. . SAMPlE MEASUREMENT t---*- - - - *-- f----- I< .----.-

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PERMIT REQUIREMENT . r--* SAMPLE MEASUREMENT r----

                                                                                                                                                           ,, .;~~'~t*l1:.1">.              ;£,,,,1('; -****~\!*~- *t.-.*-
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                   --*----**---.                                                I -*.                                                                                                                                 . '                                  .

L. ~- /N~ME/TITLE PRINciPAl EXECUTIVE OFFICER I Cert~y under penally of law that 1his document and all attachments we_re prepa. r~ under my /~ n,#, / _., TELEPHONE

                                                                                                                                                                                                                                                             -~----~ATE
                                                                                                                                                                                                                                                                             -----r--*

~

  ='                          ----~--                       d!redKmorsuparvlsJonmaccordance*MthasystemdesiQnedtoassurethatquallfieclpersonnel                                                               ~

Christopher R Church properly ga~her and evaluate the 1rVormation submll;1ed. Sased on my 1flqu1ry 01 tne parson or ~~~ 7-..--- ~ r panalt~es ---~-~---1 persons woo manage 1he system,_or 1hose persons d1rectly responsible lor galhenng !he Sequoyah Site Vice President

                           .    ,            .               1nform;;~110o. the lnlomJa1ion subfrutted is , to the best of nw knowledge and belief, true,                                                                                  423      843-7001        10      09     07 s__e_quo-yah S1te Vrce President
               ----~*

accurate, and complele. 1am aware that thens are sigrufteant for subrrlltting __ 1nformat1011, InclUding 1he poss1b1h\y of f1ne and 1mpnsonment for knowing VIOlations. felse SIGNATURE OF PRINCIPAl EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA I 1 NUMBER YEAR MO DAY J _ _TYPEOORPRINTEO _ moE..L..._. __ -*- . COMMENTS AND EJCPLANATlON OF ANY VIOLATIONS /Reference all attachments het Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPAFonn3320~~~iV3~199~1-------p~,-.-wou~--s~ed~<~km~s-m.~y~bec-u-s~ed~--------------------------------------------------------------------------~- Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilftv Namell..acattan if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ Form Approved. MAJOR

>Lame__TI/A__:_ S_EOU()_YJIH_I\IU51,1'l\R I'L~'T_ ___ _                                                                         DISCHARGE MONITORING REPORT                            (DMRI                                                       OMS No. 2040-0004 (SUBR 01)

A_cJ~_re_g)_p_,Q. B..Q.lt2.QOO_ _ _ __ _ _ _ _ _ _ _ _ _ _ _ _t[NT~~QEFJl;Uilcl85QNL _ _ -* -

  • - - - *
  • SQPQY.:..P.81SY" Tf'i.fl:!l!<!_______ _

[ P:~o~!::R II DJscHARG; ~~M~ER I ~~=~sH .E~Kjjjty_ _T\LA.:_SWY9'ffilil"JY.C!.E.A.R.1?1A..I'_H_____ _ p:rmt -~ t t 19~~tl9__1-!.Af'4!!.1Q.N_1;QY'IT(_____________ _ ---*-- -.- - -- ---[>< EFFlUENT ATTN: Stephanie A. Howard From YEAR 10 I 08MoB~ ONITjRING 01 TO 10 I !E-'!B. MQ 08 I pey 31 .- NO DISCHARGE D ... NOTE Read Instructions before completln~ ttlls rorm.

                                                                                       ---0-;;:U:-:AN=T=rr::-Y;-:O::R:-:l::O::A-::D-::IN::Gc-----,-------,O::U-cA:-:Lc:ITY:::-:O:::Rc:Cc:07N:::CE:::N:::T::R::A:::TIO'=N-- - - -
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                                                                                                                                                                                                                                              \ EX        OF      I    TYPE AVERAGE             I      MAXIMUM             I   UNITS                MINIMUM                I      AVERAGE           I    MAXIMUM      I   UNITS      l         ANALYSIS        .

SA*~lE **"'"'*"'"'"'

                                                                                                                *****"'*"'          !      *"'               **"'*"**"'             l      "'*******         l         o       I     9A       ! o-r 31T-V1Si.JAL MEASUREMI:.NI 01345       1     0      0                       /-- PERMIT                          ********                   ********                  '"'                ***"'****.*. _* :. : - .:;£'- *'"******.          .,REPORT           PAss*o t----r--;::=--*          11 VISUAL  ----1 REQUIREMEt*-
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PERMIT SAMPLE ***11*1111*

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VISUAL MEASUREMENT 84066 1 0 0 PERMIT REQUIREMENT

                                                                                     ..........          I REPORT                   YES=1 NO=O
                                                                                                                                                                                                                                     . ~.

SEE VISUAL EFFLUENT GROSS VALUE MOTOTAL PERMIT I SAMPlE MEASUREMENT PERMIT REQUIREMENT . I I SAMPLE MEASUREMENT PERMIT 1---+-- REQUIREMENT SAMPlE MEASUREMENT f-----+----1---*-

                                                                                                                                                     !;tT'f"~i:;l.;~c,~;;:;~,;(r/-*11 PERMIT REOUIREMENJ               I                     ****I*._--**-
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MEASUREMENT PERMrr f-- REQUIREMENT SAMPLE l____ MEASUREMENT o_.: ._.~.~- -- --'-----' MIT 1----f---l---- r - .... ~ ... ::~A:EIH l - .. 1 jNAME!TITLE PRINCIPAL EXECUTIVE OFFICER I Certi_fy under.pena!ty ottawthal .lhrs~ment and a. II* a_llachm~nts wef.a- praparadundermy lELEPHONE --f=-

                                                                                                                                                                                                                                                         --r--    DATE    *---~
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  ~        - - - *------ * - - - - - - -                    dire-cl.lon or superviSion 1n accoroaf!CU w1th a systl!lm designed to aSSl!re that qualified personnel                                                                    ----- ----*

Chnstopher R Church properly gather and evaluate the ntlormallon sutlm1ttea. Based on my "'qui,-y- ,;;,f :ha perscn or 1 1

                                                                                                                                                                                                                              ~~                      ~ I -~ ~

persons who manage the system, or those persons directly responSible for gathering the Information, the tnformaiiOn submilled IS, lo the best of my knowledge and belief, true, 423 843-7001 10 09 07 Sequoyah Site Vtce PreSident <UX:LI"a\e, &nd complete. Lam awar!ll that there are si_gnificant penaLttesfor submittlflQ false SIGNATURE OF PRINCIPAL EXECUTIVE . _ _ _ -----~ _ _ _ _ _ ___ informatiOn. tncludtng the posstb*llly of f1ne and 1mpnsonment tor knowing V1olat1011s OFFtcER OR AUTHORIZED AGENT AREA - NUMBER- YEA-R MO- , DAY- _ _ _ tyPEDOR_PRINTED~- _ . _ ____ __ COMMENTS AND EXPLANATION OF ANY VJOLATIONS !Reference all attachments her Operations performs visual inspections tor floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERM1TIEE NAME/ADDRESS (Include FacJJitv Name/l...ocatiOn if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Fonn Approved. DISCHARGE MONITORING REPORT IDMR) !!_am<L _ TII_A~ SE()U~~_NUCL!~ ~~N!_ __ _ OMS No. 2040-0004 Mm:~~$ JJJ.~QX_200CL ___________ _ _____ IINTE_~QE8CU6:~&SQNL _________ _ _ _ _ _ S_Q_DQ_Y __:__D_8!SL IJ~ f~ci!i~

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f----1 1--~-- PERMIT f--+-- ---1---- REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT L__ _ _ L___.L__ _ _ _,L__ __ _ L_ -------- _/ I NAMEilnLE PRINCIPAL EXECUTIVE OFFIC~ji::j' Cel1ify unde.r pe~l~y of law that \his docu. men\ ancl all attachments were prepared unaer my /. ".?' / / j TE"lEJiHONE- ---r---OATE . *-1 ~- --00,;-to_p_h-er_R_... C._hu_r_c_h______ ...  :~~;~~~~~s=a~=:~.=mv;!~~;::;ea;s=t~na:u;:~~~~i~~ C--;;? (  ?~~  ! ~-*-r--1 persons who manage lhe system, or those persons d1rectly responsible for gathenng the S&quoyah Site Vice President i~ ~J

                       .   .          .               informat*on, the 1nformat1on subm1tted is, to \he best of my knOW1Bdge and belief, true,                                                            423      843-7001          10     09        07 accurate, and complete. 1am awo~re that there a~e sigmficant penalties_ for submitting false                                                                                  ~-

quoyah Stte Vtce President _ TYPED OR PRINTEO

                                - - - - - - - - - 1nlormat1on, 1ncludlflg the possibility Of fine and 1mpnsonment for know1ng violations SICNATURE OF PRINCIPAL EXECUTIVE. I OFFICER OR AUTHORIZED AGENT          AREA  I   NUMBER rnnf. ______

YEAR - MO DAY COMMENTS AND EXPLANATION OF ANY VIOlATIONS /ReFerence all attachments he! During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 332o:1(REv-3/9-9)-------;p:-c,ec-v-:-i<Jccuc-s-e-:d:;itio:-n-sc-m=-ay-c-be-use-:-:cd;------------------------------------------- --=---------- Page 1 of 1

Mr Paul E. Dais Directcr State of Ter.nessee Depr:ment cf Environment and Conser;aticn Dtvision of Water Pollution Control 6th Floor, L & C Annex 401 Church Street, Nashville, TN 37243

Dear Mr. Paul E. Davis:

SEQUOYAH NUCLEAR PLANT (SQN) NPDES PERMIT NO. TN0026450 WRITIEN SUBMISSION REGARDING TOWERBROM 960 RELEASE TVA is providing written notification for the subject release that occurred on August 20, 2010. The verbal notification for this same release was provided by telephone to the Chattanooga Environmental Field Office (EFO) on August 21, 2010 at 1509 and to the Tennessee Emer9ency Management Agency (TEMA) on August 21, 2010 at 1510. Since the EFO telephone notification was via voice message. Stephanie Howard of TVA and Leeth a Abazid of the EFO discussed the event on August 23, 2010 Please see the Attachment (enclosed) for details of the event. If you have any questions or need additio~al information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff I certify under penally of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel property gather and evaluate the information submitted. Based on my inquiry of the person or

  • ' persons who manage the system, or those persons directly responsible for gathering the information. the information submitted is. to the best of my knowledge and belief. true, accurate.

and comprete. I am aware t/1at there are significant penalties for submitring false information, including the possibility of fine and imprisonment for knowing violations Sincerely, ..,

   ~17.~

crlristopher R. cr.ur:::h Site Vice President Seq,_:oyah Nudear Plant EnclosL!re cc (Erctosurei Chat:an::cga E~*,:r-::nr.,e.lta\ Field O~ce Divis:on cf 'v'ia:er Poi:ution Ccnt~cl State Office S*Ji:d1r.g, S:.;ite 55C US Nwc:e3~ Re~:.:!a:Jry Co~r.:ssi-Jr

      ;,nN. DscL.:;;e--,~ Cor:~.~o1 Oe.s.i 1//ashir.g:::r._ 0 C 2*J555

Attachment Description of the Discharge and Determination of Cause: At 1850 or: 8i20110. piar:t sect.;ritJ f:erscn~e: cbser;ed a leak adjacer.t ~o tr.e Essentiai Ra*.v Cccling 1/'/ater {ERGN) t:~ildir*.g at the Se(iUOJa~ N~Jdear Plant (SON). The pumps in t~is c~iidir.g trar.sfer Ter.nessee R*Jer wa!er ir,to tr,e plant fer ccoling pt..:r~cses. The lea~ W3S st:rafng fro!T, an cuts:de cf":er:-:ica: i~iect:"cr pipe fr.ttirg drair: ~ll.ig, cver.lcwing a smail t:asir. cr:to cor:crete. a.--:d o~1er a 1e~;e irtc the Ter:nes2es R:ver. Perscr.ne! w:re dis~a:cr.ed to ir.vest:s;a:e Tr.e lea{ was Cetsr~:r.ed tote trea:ed ERS'*J'.i Tr.e :r.em:ca~ used to trea: the ERC'// is Tc*11ertr:Jm 9CO. a r:-:ix~:.Jre cf scc!ium dicr,icr-:)isccyar.Lra~a ar:d scdit,;:-71 t:r:r.,:de The ~re:ir:"'.i~a~/ car-.:se cf the reak is the failure of tr.e drain pi~S GL;e tc corrosicn_ Period of Discharge: The chemical feed was initiated at 1650 cr. 8,20/10 ar:d discovered at 1850 on8/20/10. The treatment chemical feed was isolated at 1955. Therefore, the duration of the leak is bounded by a minimum of 65 minutes to a maximum of 185 minutes. A sample of water in the bottom of the catch basin was analyzed and determined to contain 55 ppm total residual chlorine. The permit limit at Outfall 101 is 0.1 ppm chlorine (total residual). No reportable quantity per CERCLA was exceeded. TDEC notification satisfied Federal insecticide, Fungicide and Rodenticide Act (FlFRA) requirements. An estimated 180 to 540 gallons of treated water was spilled. No other chemical release to the Tennessee River occurred. Steps Being Taken to Reduce, Eliminate, and Prevent Recurrence When site emergency responders arrived at 1950 on 8/20/10, a spill response catch basin was placed under the leak location, capturing approximateiy 125 gallons. The strainer water feed was isolated at 2053. At this point, all fiow was terminated. The cause of the leak is being investigated and, once determined, permanent corrective actions will be taken. SR 236979 was written to document the incident and corrective actions. Currently ERCW chemical additions have been temporarily suspended until a full extent of condition including examination of other drain plugs can be completed. A system waik down was completed 8/21/10 to verify proper system alignmant. No misalignments were found. Metallurgical examination of the drain plug is in progress to determine the exact cause of failure. Other Details: No fish distress fish mortality, or abnormal bird migration was observed by site personnel the evening of the event and the morning following. The afternoon of 8/21/10, TVA Biological Resource Staff visually surveyed the near shore area by boat two miles upstream ar.d four miles downstream of the Plant for any signs of dead or dying fish, cr aquatic wildlife. During the investigation, water fowl (two groups of 8 and 6 cf Canada Geese), three Great Blue Herons ar,d tNo Cormorants were observed. No dead or dyir.g fish or affected wildlife was observed in the search area. The opposite shore line was also surveyed to 1/erlf'J any effects of the biocide spill No dead or d:ting fish or affected wildlife was observed.

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT -August 2010 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Bethany Hyatte DATE: 9/8/2010 CONCURRENCES Name R C Signature - Comment Date V N S_ A. Howard G.J. Wynn B_ A. Wetzel I K. Langdon tJ- tf'-1 D C. R Church X '~7~ U INSTRUCTIONS: Originator will determine the review/concurrence assignment REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 101012 800- NPDES CORRESPONDENCE October 12, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1 6 " Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR SEPTEMBER 2010 Enclosed is the September 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. On September 22, 2010 at approximately 2130 EDT, a fire occurred while placing a non-polychlorinated biphenyl (PCB) transformer in service following routine maintenance. As a result of the fire, approximately 13,000 gallons of insulating oil spilled from the transformer into the surrounding gravel switchyard. The secondary containment for the switchyard is the Yard Drainage Pond. A permanent oil skimmer is located on the discharge of the Yard Drainage Pond prior to entry into the Diffuser Pond. TVA mobilized its Emergency Response Team and began recovering the spilled mineral oil. As of today, no oil has been released into Waters of U.S. (e.g., Diffuser Pond). This has been verified by both visual examination and oil and grease analysis. Cleanup continues at both the Yard Drainage Pond and the switchyard where the spill occurred. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely,

/~:?_/:/(

Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Envitonmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control ATIN: Document Control Desk State Office Building, Suite 550 Washington, D.C. 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc: B. E. Brickhouse, LP 5U-C A. A. Ray, WT 11A-K C. R. Church, OPS 4A-SQN G. R. Signer, WT 6A-K S A. Howard, OPS 5N-SQN B. A. Wetzel, OPS 4A-SQN K. Langdon, POB 2B-SQN G. J. Wynn, POB 2B-SQN D. B. Nida, LP 5U-C K. M. Hodges (EDMS), LP 2V-C

Tennessee Valley Authority, P:Jst Office Sex 2000, Soddy DaJsy. Tennessee 37384-2000 October 12, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6'" Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR SEPTEMBER 2010 Enclosed is the September 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. On September 22, 2010 at approximately 2130 EDT, a fire occurred while placing a non-polychlorinated biphenyl (PCB) transformer in service following routine maintenance. As a result of the fire, approximately 13,000 gallons of insulating oil spilled from the transformer into the surrounding gravel switchyard. The secondary containment for the switch yard is the Yard Drainage Pond. A permanent oil skimmer is located on the discharge of the Yard Drainage Pond prior to entry into the Diffuser Pond. TVA mobilized its Emergency Response Team and began recovering the spilled mineral oil. As of today, no oil has been released into Waters of U.S. (e.g., Diffuser Pond). This has been verified by both visual examination and oil and grease analysis. Cleanup continues at both the Yard Drainage Pond and the switchyard where the spill occurred. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely., /_

 //~t:u

~h;rR. ~h~rch Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control ATTN: Document Control Desk State Office Building, Suite 550 Washington, D.C. 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLLJTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved DISCHARGE MONITORING REPORT (DMR) m~--~~~~~~~~~~P~~-- (SUBR 01) OMB No_ 2040-0004 lld!lrtlli _p..Q.Jt0JL2JK)O_ - - - - - - - - - - - ____IIJILTEBQEFEE.SIU&S!lNL _______ _ F- FINAL -* ____SQPQY _:_D_AIS_'L_ TN 27~- ______ _ DIFFUSER DISCHARGE ~cm~~~~~~~~~~~m ____ _ EFFLUENT !.Qta_ti_Q_ _H_8_M_llT_Q_I\LCQY~IX- _ --* _ _ _ _ _ _ _ _ ATIN: Stephanie A. Howard NO DISCHARGE D ... NOTE: Read instructions before completinQ this form PARAMETER QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE

                                                                                                                                                                                                    --* -*                     EX           OF         TYPE AVERAGE                MAXIMUM                UNITS                 ANALYSIS TEMPERATURE, WATER DEG.                                                                                                                                               ********                29.0                                0       30 I 30     MODELD CENTIGRADE 04 00010         z    0    0                                                                                                                                             ********                30.5                 DEG.C.                   SEE        CKREQ INSTREAM MONITORING TEMPERATURE, WATER DEG.

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                                                                                                                                                                     .... b              P~II,Y_M_lL 04          0 PERMIT 30 I 30     RCORDR 00010        1    0    0                                                                                                                                             ***"lrir***          REPORT                  OEG.C.                    SEE       CKREQ EFFLUENT GROSS VALUE                                                                                                                                                                     PAILY M_J{                                     PERMIT TEMP. DIFF. BETWEEN SAMP. &                                                                                                                                           ********                 2.2                                0       30130        CALCTD UPSTRM DEG.C 04 00016              s    0                                                                                                                                             ********                 3.0                 DEG.C.                CONTIN        CALCTO EFFLUENT GROSS VALUE                                                                                                                                                                     DAILYM>L                               *----

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                                                                                                                                                                                                  -*- **-. -*--       ..     ' 0         30/30 RCORDR 50050             0    0                                                                                                                                             ********               **"'"'**"'*                                 CONTIN       RCORDR EFFLUENT GROSS VALUE                                                                                                                                                                                                                      uous NAME/TITLE PRINCIPAl EXECUTIVE OFFICER              1Certify under penalty of taw that this document and all auactunents were prepared under my              .,/**-   ~*- -~u                                     TELEPHONE             I         DATE directton or supervision tn accordance wtlh a system destgned to assUie that qualified personnel     _/ ~                ~              / . _, '                                    I       I     ----r---~

Christopher R. Church property gather and evaluate the tnformation submitted. Based on mytnqutry of the person or / ~ L ~ c ( .--/ /

                                                     'persons who manage tile system,. or those persons d~recl!y resp.onSJble tor galhenng the              Sequoyah Site Vice President information. the Information subm11ted IS , to the best of my knowledge and belief, true,                                                                 423       843-7001           10 I 10      I 07 Sequoyah Site Vice President                accurate, and complete. I am aware that there are &ignificant penalties for submittit)Q false      SIGNATURE OF PRINCIPAL EXECUTIVE 1-------=c=::=cc:c=-:::-::ccc:=c-------jinlormalion, including lhe possiblltly of line and Imprisonment for knowtng violattons.                              OFFICER OR AUTHORIZED AGENT                       AREA    I    NUMBER         I YEAR I MO I DAY I TYPED OR PRINTED                                                                                                                                                                                     L__                  L      .        .       .

cOM_M_E_N_T_sANDEXPiANATiQN':ocF:-:AcNcY-:-:-VciO:c-LA-:::T~Io=-Ncs:---,R--:-ecfec,-e-n_c_e_acllca-cttcac--:ll-m_e_n_ts-cnce-,----------------.L------------------_L-".ll.l"- - *--- No closed mode operation. The following information is included in an attachment: 1. CCW data 2. veliger monitoring data EPA Form 3320*1 (REV 3199) Previous editions may be used Page 1 of 2

DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis DatefTime Analyst Method No water would come out of the pump. No sample could be obtained. CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 09/15/2010@ 1025 <0.10 mq/1 09/17/2010@ 0108 JOB EPH

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp. ('C) Temp. ('C) LOCATION Clams/m3 Clam 11/03/2009 133 0 16 11/03/2009 76 16 lnplant RCW CMW 11/10/2009 417 6.1 16 11/10/2009 25 16 lnplant RCW CMW 11/17/2009 269 0 16 11/17/2009 0 16 lnplant RCW CMW 11/2412009 36 50 15 11/2412009 18 15 lnplant RCW CMW 12/01/2009 32 0 13.5 12/0112009 0 13.5 In plant RCW WE 12/0812009 38 0 11 12/08/2009 0 11 In plant RCW CMW 01/0512010 0 0 6 01/0512010 0 6 In plant RCW B 01/12/2010 0 0 5 01/12/2010 0 5 In plant RCW 01/19/2010 0 0 6 01/19/2010 0 6 lnplant RCW p 01/26/2010 32 0 7.5 01/26/2009 0 7.5 lnplant RCW NRT 02/02/2010 0 0 7 02/02/2010 0 7 lnplant RCW MSW/WDT 02/09/2010 0 0 8 02/09/2010 0 8 lnplant RCW BLITC 02/16/2010 0 0 5 02/16/2010 0 5 In plant RCW BJ 02/23/2010 11.7 0 7 02/23/2010 0 7 lnplant RCW BJ 03/02/2010 0 0 6 03/02/2010 0 6 lnplant RCW PB 03/09/2010 0 0 8 03/09/2010 0 8 lnplant RCW MJW 03/16/2010 0 0 10 03/16/2010 0 10 lnplant RCW BC 03/23/2010 14 0 11 03/23/2010 0 11 In plant RCW BC 03/30/2010 0 0 14 03/30/2010 0 14 In plant RCW BAPO Apr-2010 no samples collected May-2010 no samples collected Jun-2010 no samples collected Jul-2010 no samples collected Aug-2010 no samples collected Sep-201 0 no samples collected

PERMITTEE NAME!ADDRESS (Include Factlify Name/Location tf Different) NATIONAL POllUTANT DISCHARGE EliMINATION SYSTEM (NPDESJ Form Approved MAJOR DISCHARGE MONITORING REPORT (DMR) }!a!!le__TY_A_:_ SEOIJOJ~_tiiJ~!_,E:AR I'_LANT___ _ (SUBR 01) OMB No. 2040-0004 Mill:~ __p_.O.J:!OX200Q _______ ~-* -*-- tlNTERQFF!C:E;_SQ.:...28.-:.SQ:NJ_ -~ _ _ _ _ __ f TN0026450 j ~01 G -~ F- FINAL _ _ _ _SQ!J!!Y _-_DI\lSY.* IN 27~4_ _________ _ f_gctJjty_ _ _T.\LA_:_S.E:.OUOYAH NUC!.!;.AR. PbArfT __ .lQ@t!Q __HAfl.!!bT_QN.J;our*u:v_ _ --* _ _ _ .- ~-* _ -* ATTN: stephanie A. Howard From __ _p_ERMJIJ\IUM!!ER_. b_o B9_r o;j 1 AR MQ ~; QRIN.G_&l To ufu~_i__3o~

                                                                                                                                                                                                               ----1 J'!UM!lER.

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                                                                                                                                                                                                                             *          -::-z-;~_.7~;>-/--~TELEPHONE-n
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k ~>u*~~-*ro~~ --~t'"""_bdw>10.i Christopher R. Church properly gather and evaluate the 1nformallon submllled Based on my InqUiry of the person or persons who manage the system, or lhosa persons directly respons1ble for gathen11g the 1nformat1on, the mformallon subm1lled 1S , to lhe best of my knowledge and belief, true. __ Seq~oyah Site Vice Pn:_~i~~-~~----*- 423 843-7001 I 10 10 07 Sequoyah Site V1ce President accurate. and complete 1am aware that there are s1gmf1cant penalties for subm1tt1ng false SIGNATURE OF PRINCIPAl EXECUTIVE

      .-.-*---------------*----                             InformatiOn. 1nclud1ng the poss1b1hty of fme and 11nprlsonmen1 for knowmg Vlofa11Dns TYPED OR PRINTED
                 --~--*-*--    -*--- -*~-----

COMMENTS AND EXPLANATION OF ANY VIOLATIONS tReference all attachments her The following injections occurred: 1. Floguard MS6236 (max. calc. cone. was 0.038mg/L-~Iimit 0.2mgll) 2. Spectrus CT1300 (max. calc. cone. was 0.040mgll--limit 0.050mg/L) 3. Spectrus CT1300 (low detection level analytical method was <0.050mg/l--limit 0.050mg/L)

          -------~

t:t"A t"orrn .J320*1 {REV 3199) Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) !LamL _ "fl!.A.: s~:quov~H_I'jUCLE~R I'LANT___ _ (SUBR 01) OMB No_ 2040-0004 P._9Qr~~:? ___ P,Q. __6QX_2_@Q_ ___________________ _ ____(L~J<.~Qf~!CUil."85QNL __________ _ SQPI~Y .* Pl\JSY, TN .~?~!l4__ _ TN0026450 -l ffRMlT.miMBfR____ LJ:liSQiARG.E.N!!M6ER 101 Q F- FINAL D(FFUSER DISCHARGE fiK!!i\Y.. _TY11.:.5EQI.!QYI\IiJillCLfA.R~l..AJIIT _ _ *- Frambi"l~~JWf~~Rmff~ EFFLUENT !Qc_atjQ___HAMUJQN.CQUN.TY ATTN: Stephanie A. Howard

----PARAMETER                                               *k~                          . * -. ..            OUANT!TY OR LOADING                                   ,---*-

__l_ QUALITY OR CONCENTRATION NO DISCHARGE r~MAXIMUM [J *** NOTE: Read instructions before completm!=l this form

                                                                                                                                                                                                                                                                 ------  ------~---  ........ - - - - - - - * -*- --.-- ..

NO. FREQUENCY SAMPLE EX OF ANAlYSIS TYPE

~:::~: ~:~~.~~

UNITS MINIMUM AVERAGE UNITS feO-RON, r6TAL________ -------- sAMP-LE-- MEASUREMENT

                                                                                                                                                             ..                                                   <0.20                                                       19 0

3/92 f-- *-* ---~---* ------- 01022 1 0 0 PERMIT **** oll)illr11

                                                                                                                               "'***"'***                   *"'**           *******                           REPORT                                  **"'****              MG/L                    QTRLY            GRAB REQUIREMENT EFFLUENT GROSS VALUE
-----~------------~-             --** *~-* -----------                                                                                                    ~-         --                                                                                                      ------  --~---.

SAMPLE MEASUREMENT

                                                             ---;;* *------ -------,---------,-----:-- ----. -,--        -- ----------------                                                                                    - - -------                  - -  ~-                        *-   - - - - - r--*--**

PERMIT REQUIREMENT I . *-- SAMPLE MEASUREMENT I'

                                                                                                                                         ..                                                                                                      --**-~--c--

PERMIT *** ** REQUIREMENT . . ** .. . .. .* .. * .* I-* ....... ***--------~ SAMPLE MEASUREMENT PERMIT .** 1-*-.- .. - . - - - --------- ---- 1- . ---~ . - **----*- .. REQUIREMENT . I *: . :. ,.: *. *.*... 1**.:::._. I SAMPLE MEASUREMENT PERMIT

                                                                                                                                                                      ~

r---- I

                                                                                                                                                                                                                                                                                                                       -~

REQUIREMENT ...*.* . .. r--- SAMPLE MEASUREMENT

                                                              --PERMoT*--
                                                                                         ---~-..,--~------   __,__ "".    --*--.-*-            ---*-  ---                                                                                          --~-*----                                      - - - - ------

REQUIREMENT . . .. . I .. ~- - r-----1= SAMPLE MEASUREMENT PERMIT REQUIREMENT

                                                                                                                                                                                             .z;- #:~1-
                                                                                                                                                                                                                                                                         ~----~--

NAME/TITLE :PoROICNCC::CIPCACL:-EXEC_U_T_IV_E_o_Ff=ICER Christopher R. Church I Cert1fy under penally of law that this documem and all altachments were prepared under my d1rect10n or superviSIOn In accordance w1th a system des1gne<:1 to assure that qualified personnel properly gather and evaluate the intormat1on submitted Based on my inqu1ry of tt1e person or p~rson~ who manage llle syslem, or !hose persons dnectly respons1ble lor gathenng the _TELEPfjONE ***;1~-~~~::.:?:x.:_E~..:: .. Pres~ent___

                                                                                                                                                                                                                                                                        --j----- ~0 1

Sequoyah Site V1ce President t=-----TY*P*E--~~-~~~-T_E_D

                                           .          .                1nformalion, the 1nlonnallon submttted IS, lo the best of my knowledge end belief, true, accurate, and complete_ I am aware that !here are s1gn1ficant penalties lor subm1t1ing false poss1~~~: :~=:~~~~::i~:~=~~~~~r know1ng violations.

1-1 Sequo_yah Site Vice

                                                                                                                                                                                         --;:S;;;IG:CNCCAT;;U;;;RE OF PRINCIPAL EXECUTIVE
                                                                                                                                                                                                                                                                        .423      843-7001
                                                                                                                                                                                                                                                                                                      -*- *- * **10        07- _

1nformat1on, Including lhe OFFIC~~~~~-~~~~~IZ~~-~~-~- -~~~ ~-~~~R YEAR MD DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS tReferencea/lattachments her Boron was sampled on 717110.

               -*--** *-****----*-** --    ~-------~-

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMIITEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAl POllUTANT DISCHARGE EliMINATION SYSTEM (NPDESJ Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMRJ ~~--~~~~ov~~uc~~~~~--- (SUBR 01) OMB No_ 2040-0004 Mdress _p_,_Q._BOX.:;:ggo______________ _ ---~m~~~ES~~~L ______ _ I TN0026450 101 T F- FINAL ---~ID~M~rn~~L ______ _ BIOMONITORING FOR OUTFALL 101 fill;;.i.!.i(Y__T~_:__SEQUQ'ffilf_l..IUCLEA_R_el_A.N_T_ _ _ _ _ EFFLUENT ~~~-~~m~~=----------

                                                                                                                                                                                            *** NO DISCHARGE             D ...

ATTN: stephanie A. Howard

                                                                                                                                                                          -   I  "=--"             NOTE: Read instructions before completint:~ this form.
                                                  ~

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE UNITS ANALYSIS MAXIMUM IC25 STATRE 7DAY CHR

                                                                                    ********                      ...........            -             Monitoring            ********

23 CERIODAPHNIA TRP3B 1 0 0 MEASUREMENT PERMIT ........ *-***** .... Not Required 45.2 ******** ******** PERCENT SEE COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM PERMIT IC25 STATRE 7DAY CHR SAMPLE ******** ******** Monitoring ******"'* ,., MEASUREMENT 23 PIMEPHALES Not Requir~d TRP6C 1 EFFLUENT GROSS VALUE 0 0 PERMIT REQUIREMENT

                                                                                    ********                      ""**"'****            *-*                 45.2

_IIIIJMINUM._

                                                                                                                                                                             ********                 ********       PERCENT                      SEE PERMIT COMPOS
                                                                                                                                                                                                                --                            --------~-
                                                                                                                                                                                                                                                            ---~------

SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT I-- REQUIREMENT

                                                                                                                                                                                                                                         ---  ---------     -~----

SAMPLE MEASUREMENT PERMIT REQUIREMENT

                                                                                                                                                                                                                                     **-*      -------      -        --~-

SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT L__ N_AME/TIT~E PRINCIPAL EXECUTIVE OFFICER 1 Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supen~is1on in accotdance w1th a system des1gned to assure that qualified personnel Christopher R. Church properly gather and evaluate the information submitled. Based Oil my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the 1nformat1on, the information submiHed 1s , to the best of my knowledge and behef, true, 423 843*7001 10 10 07 Sequoyah Site Vice President accurate. and complete. 1 am aware that there <Ire significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE f finfor.-nation. including /he passil:>ili!y of fine and imprisonment for knowing Yiolatioos_ OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments het Toxicity was not sampled in September 2010. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

(Include Facility Name/Location if DifferenfJ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ PERMITIEE NAME/ADDRESS MAJOR Form Approved DISCHARGE MONITORING REPORT {DMR) m~--~~~~~~~~~~~~~--- (SUBR 01) OMB No. 2040-0004 Mm~ _FAL!lPX2Jl!)Q_ _ _ _ _ _ _ _ _ _ _ _ 5 1 P;R%~i~~t B~R J I DISCHARGE ~~Mtd F- FINAL ---~~ill~~~~~~~------- ---~@ID~~~lli2~L ______ _ [ LOW VOL. WASTE TREATMENT POND

          .~lVA:.....SEQUQYAt:LNJJClEAR et,_AN_T_ _ _ _                    _

_Eact)jw_

                                                                                                                                           ~QJ\IITDRING            PEiiof          ---~

EFFLUENT ls;ti ~.~ 11b11 ~~~~A~~~~---------- 1 ATTN: stephanie A. Howard From p~~R I ~~ r11 I TO

                                                                                                                                                                                                             *** NO DISCHARGE
                                                                                                                                                                                                                                         . .D           ...

NOTE: Read mstruct1ons before completinQ thiS form. PARAMETER ,--~-------Q::cU:-:A-:clc:IT=v=o=R-:C::O:cN::C=EN:cT:':RccAC:T::'IOO':N:,:::::::..::::._ NQ FREOUENcVTSAMPLE*- EX OF . I TYPE

                                                                                                                  .. ft.........       .****-              MINIMUM                       AVERAGE                      MAXIMUM                                     ANALYSIS PH                                                                                                                                                              7.2                       ********                              8.4               12        0      15 I   30     GRAB 00400                0      0                                                                                                                                                             **-II*****                            9.0               su               THREE/        GRAB
                                                                                                                                                                                                                 . MM<!!\II_U_!\11__                               WEEK 12                                 15              19 1  0r-S-J30-I            GRAB 00530                0      0                                                                                                                               ******"'*                          30                              100               MGIL   I       I WEEKLY I       GRAB EFFLUENT GROSS VALUE                                                                                                                                                                     IV!O A,_VG                  DAILYMX~-1 OIL AND GREASE                                                                                                                                               ********                                                                                   1    _ , __  5/'So       GRAB
                                                                                                                                                                                              <5                                 <6              19        0 00556                0      0                                                                                                                               **"'*****

MOAVG 15 DAILYMX 20 MGIL WEEKLY l GRAB 301 .:;u TOTALZ

                                                                                                                                                                                                                                                 **        0
                                                                                                                                                                                         ******'**             I       ........ **                                             TOTALZ
                                                                                                                                                                                                                                            +-----1---*1---------+------1 t
                                                                                                                                                                                                                                                        ~--+-----+-~

f---~---j~~--t~~ _____ [~-~-~-+ ~

                                                                                                                                                                                            -~

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this documenl and all attachments were prepared under my ~:;-/-: I TELEPHONE I I DATE

                                                                                                                                                                                          ?/'-" /l,~

drrection or Sl.lpervision in act:ordance with a syslem designed to assure that qualified personnel Christopher R. Church properly gather and evaluate the Information subm1tted. Based on my InqUiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Sequoyah Site Vice President information, the 1nlormahon submilted is , to the best of my knowledge and belief, lrue. 423 843-7001 10 10 07 Sequoyah Site Vice President accurata, and complete. I am aware that there are significant penallies for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE 1 - - - - - - - - - - - - - - - - - - - - - - l ' i n f o r m a t i o n , including the possibility ol !me and impnsonment for know1ng IIIOietions OFFICER OR AUTHORIZEO AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments het EPA Form 3320-1 fREV 3199) Previous edJ1kJns may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. DISCHARGE MONITORING REPORT (OMR) Rame_ _T'!_A_:_ ~E()U()YII_H_tll)C[1;~ ~LII_N.!_ _ _ _ (SUBR 01) OMB No. 2040-0004 MQreM_p.JJ.JtOX2.000_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _CIN_TERQEOCUS:28.:.S.QNL. _ _ _ _ _ _ _ TN0026450 ~ 107~ F- FINAL ______SQPID' ..cPl\!S'L I!\! 2?~- ______ _ PERMIT NUMBEHARGE JI!!JMllR METAL CLEANING WASTE POND B!Q!!\lL _Tlll\~fllUQ'illliNUClEARI'U\RT_ _ _ .LQcatiQ_ _H_ll.~MJb1QN_CQU~IY- ________ _ ~~T RING ... P~ ~ EFFLUENT ATTN: Stephanie A. Howard From\10 YEAR I MO 09 r01 j rohoJ MO 091; P

                                                                                                                                                                                              ... NO DISCHARGE            [x_Xj ***

NOTE: Read instructions before completinQ th1s form. rr;K).- FR~*OUENCY X PARAMETER QUANTITY OR LOADING QUAliTY OR CONCENTRATION SAMPLE

                                                                                                                                                           ..                                                                       EX              OF          TYPE ANAlYSIS PH                                                   SAMPLE MEASUREMENT AVERAGE                    MAXIMUM UNITS MINIMUM               AVERAGE MAXIMUM            UNITS 12 00400       1    0        0                           PERMIT                     **-****                    ********                ****     --6.0                            ****"'***                   9.0-             su                      DAILY        GRAB REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                              MINIMUM SOLIDS, TOTAL SUSPENDED 00530      1    0        0 SAMPLE MEASUREMENT PERMIT
                                                                                                                                    --      r-----::**-********
                                                                                                                                                                             **""**"'*~~'

MAX!NIUM - 30 19 MG/L

                                                                                                                                                                                                                                       .....L.
                                                                                                                                                                                                                                  !---~DAILY
                                                                                                                                                                                                                                                         -*  -***-***-*~--

COMPOS REQUIREMENT EFFLUENT GROSS VALUE . _j)AILYMX ----- OIL AND GREASE SAMPLE *****-* ******** **~~'***** ****~~'*~~'* MEASUREMENT 19 00556 1 0 0 PERMIT

                                                                                **-****                    ***-***                 .....               ............          *****~~'**

15 MGIL DAILY GRAB REQUIREMENT I EFFLUENT GROSS VALUE

                                                                                .........                                                              ......                                       DAILY NIX_

PHOSPHORUS, TOTAL (AS P) SAMPLE MEASUREMENT lrfr****~~'*

                                                                                                                                     -                           ;;* -~~     ****~~'*~~'*

19 00665 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT

                                                                                ********                    ********               -**                 ********              *****"**                     1.0

__ PA!LYMX MG/l DAILY

                                                                                                                                                                                                                                               --*~--

COMPOS COPPER, TOTAL (AS CU) SAMPLE ******** ******** ** **~~'***** ******** MEASUREMENT 19 PERMIT ******** **** ********

                                                                                                                                                                                                                   --    MG/L        .

DAILY COMPOS 01042 1 0 0 REQUIREMENT

                                                                                ***'*"****                                                                                   **"'**"'**                   1.0 EFFLUENT GROSS VALUE IRON, TOTAL (AS FE)                                  SAMPLE MEASUREMENT                   ******-                     *******"'
                                                                                                                                                        **~~'**~~'**         ********

DAILYMl< .. 19

                                                                                                                                                                                                                                    -~-~-     - - - - - ,....---

01045 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT

                                                                                                            ******-                                    ********              ******~~'"'

1.0

                                                                                                                                                                                          - - - _PA!L'f_NI)(

MG/L DAILY COMPOS FLOW, IN CONDUIT OR THRU SAMPLE MEASUREMENT 03

                                                                                                                                                                             ********                 *"'*~~'*~~'**        ..               -  --***-*-- f - - - -

l .. TREATMENT PLANT 50050 1 0 0 PERMIT REPORT REPORT MGD ******** *~~'****~~'* ******** DAILY CALCTD REQUIREMENT EFFLUENT GROSS VALUE MOAVG DAILY MX

------                                                                                                                                                                           ~                                    - - - - - *-*-

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penalty of law that this document and all allachments were prepared under my TELEPHONE I DATE Christopher R. Church direction oc supervision in accordance with a system deSigned to assure that qualified personnel properly ga\her and evaluate the information submitted. Based on my inquiry of the person or %1~

                                                                                                                                                                                 .__.. !!-(    ~       -*~.

persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice Pr *ide7r 1nformat1on. the Information submitted is , to the best of my knowledge and belief. true. 10 I 10 I 07 Sequoyah Site Vice President accurate, and complete. I am aware thatlhertt are significant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE 1nlormat10fl. Including the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT YEAR I MO I DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments he! No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include FaCIIirV Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Fotm Approved. DISCHARGE MONITORING REPORT (OMR) JILamc ~fl.'A_- S~~U()YJI_H_NUCLJ;AR I'IJINl'_ ~ ~ ~ (SUBR01) OMB No 2040-0004 _P..Q.J!PX2000_ _ _ _ _ _ _ _ _ _ _ _ Ad!1re~ ~~~~~~~~u~~~L~~~~~~~ ___ --~SQPQY _*_PI\JSX~ L~ 27~4 _______________ _ I TN0026450 PERMIT NUMBER II DISCHARGE N 11 ~ F ~FINAL RECYCLED COOLING WATER ~@~J~~~~M~~~~w ____ _ b~R I ~~ n~TJ~:~p;~j ~nWJ EFFLUENT LO~~~~m~~=~~~~~~~~~~ From NO DISCHARGE [Xi] ... ATTN: Stephanie A. Howara NOTE: Read mstructions before completinQ this form. PARAMETER QUANTITY OR LOAOINC QUALITY OR CONCENTRAl1uN ~QTFREOUE.NCV]'-sA_M_P-LE~ EX I OF ANALYSIS I TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM TEMPERATURE, WATER DEG. SAMPLE ******** *******"' ******** ******** CENTIGRADE 00010 z 0 0 MEASUREMENT PERMIT REQUIREMENT 04 DEGC ******** ******** 38.3 04 DEGC DAILY GRAB-4 INSTREAM MONITORING PH SAMPLE MEASUREMENT OAIL'LMl<__i 12 i--*~ __,_ --------

                                                                                                                                       /--- 6.0
                                                                                                                    -~- "
                                                                             *******"                                                                                                       9 II                          I 00400          0     0                             PERMIT                                                 ********                                                   *"'*****"'

REQUIR"* ~*~ REQUIREMENT EFFLUENT GROSS VALUE SAMPLE ******** MINIMUM .0~~- su WEEKLy GRAB Lc

                                                                                                                              -                                     ***"'****         MAXIMUM                          ________ ,_ ----
                                                                                                                                                                                                                                               ~~" ~

SOLIDS, TOTAL SUSPENDED MEASUREMENT ******** ******** PERMIT **-*-* 19 . --

                                                                                                                                              ........                                                                                  DAILY~          -~ ~:::

00530 0 0

            ~~~---~~-~-

EFFLUENTREASE GROSS VALUE SAMPLE

                                                         ~*                                              ********            H..                                                             30            I   MG/L               I                 --"

OIL AND G MEASUREMENT .......... ******** ........

                                                                                                                                                                    ********            - ----~-,-----

DAILy MX *'" 19 00556 0 0 PERrtMT ******** ....,. . . .... .......,. .....,... I 15 MGIL *-~*DAILY I GRAB I ~~~~W:~~~~:~~~T~~~~RU RE::;:r ... **... ---;;-;*****;*----- _J~~~~~K .. ~*~~~~ -----1 TREATMENT PLANT MEASUREMENT 03 50050 1 o 0 PERMIT REPORT REPORT MGD ******** ******** ******** uu DAILY I CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVC. DAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE **-***"' ****"'*** I .... I ******** I ,-***-;,..... ------------- 19

                                                                                                                                                                                                                      +-- --

l MEASUREMENT 50060 1 0 0 PERMIT *****u* *******"" ***..-.**"' **"*""*** 0.10 MGIL WEEKLY I GRAB-4 EFFLUENT GROSS VALUE REQUIREMENT DAilY MX

                                                                                                                                                                 ""-~"--          -  ~~       --~      ."              I                         +~~-

SAMPLE MEASUREMENT PERMIT REQUIREMENT

                                                                                                                                                                       /

NAMEniTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law thai thiS document and all attactlmenls were prepared under my TELEPHONE~ DATE direction or supervision In accordance Wl\h a system des1gned to assure thai quail! ted personnel Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of 1t1e person or persons who manage the system, or those persons diredly responsible foc gathenng the Sequoyah Stte Vice Prestdent

                                   ,              Iinformation, the information submiUed is, to the best of my knowledge and belief, true, accurate, and complete. 1 am aware that there are stgmficant penalties_ for submitting false 423      843~7001            I 1o I 10 I 07 SIGNATURE OF PRINCIPAL EXECUTIVE
                                                   ~nfOfiTia\ton, tncludtng the posstbilLty of fJne and tmpnsmment for knowtng v1olallons.

OFFICER OR AUTHORIZED AGENT NUMBER I YEAR I MO I DAY I -~---'TYPED OR PRINTED L. COMMENTS AND EXPLANATION OF ANY VIOLATIONS /Reference all attachments her No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) m~--~~~~OY~~~~~~~~--- (SUBR 01) OMB No. 2040-0004 APmE;lss __p.Q.JlOJt2.000_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _tiN_TE_RQEF_l_C~~A-SQ_NL _ _ _ _ _ _ _ _ _ ___ 3WID~m~rn2~L ______ _ I TN0026450 PERMIT NUMBER I ~0

                                                                                                                                                   ~UMBER T I     F- FINAL RECYCLED COOLING WATER f~Q_ljt;y __T_V_1\_:_SEQY.QY8H_____N!,K:U_A_R_eL_A_W_ _ _ _        _

Iyi~ I ~~ r~~~~: r~l ~~ I ~ag I EFFLUENT ~~~-~~N~~~---------- From NO DISCHARGE [-XX) *** ATTN: stephanie A. Howard NOTE: Read instructions before completing thiS form QUALITY OR cONceNrRATIOt.i - - - - - - - - - ~ FREOuENCv X PARAMETER QUANTITY OR LOADING SAMPLE I EX OF TYPE ANALYSIS IC25 STATRE 7DAY CHR CERIODAPHNIA SAMPLE MEASUREMENT AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 23 TRP3B 1 0 0 PERMIT REQUIREMENT

                                                                               ***'***-                  ..........            *-*                 45.2             "'**"'*"'**        "'*****"'*      PERCENT SEMI         COMPOS I EFFLUENT GROSS VALUE                                                                                                                            MINIMUM                                                                          ANNUAL IC25 STATRE 7DAY CHR                                   SAMPLE                                             ********                                                   ********           ********

PIMEPHALES MEASUREMENT 23 TRP6C 1 0 0 PERMIT ******** ******** ..... 45.2 *"**"**" *"***"*" PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL

                                                                                                                                                                                                  *-*- ~~--          ***-~    ---*---

SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE

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1-- c--- MEASUREMENT PERMIT REQUIREMENT

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                                                                                                                                                                       -*                                                   - -  --* -*---* -- *--~--

SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

                                                                                                                                                                      /"

I j_ - I NAME/TITLE PRINCIPAL EXECI}_!!_~E___q_FFIQ:R I Car11fy under penalty of law that !his document and all aUa~;:hmenls were prepared under my direction or superviSIOn in accordance with a system designed to assure that qualified personnel I TELEPHONE I DATE Christopher R Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persor1s who manage the system, or those persons directly responsible for galhenng the 0 'I'L-U:;, Sequoyah Site Vice President mformat1oo, !he Information submilted is , to !he best of my knowledge and belief. true, 07 Sequoyah Site Vice President accurate, and complete I am aware that there are signif1cant penalties for submi1ting false SIGNATURE OF PRINCIPAL EXECUTIVE 1--------:::-:::::::-::c::-::=::-::=:-------jrnfOfmation, Including the possibilily of fine and 1mprisonrnent for knowing Violations. OFFICER OR AUTHORIZED AGENT DAY I TYPED OR_,IN~ED -~--- ___j COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments her No Discharge this Period EPA Form 3320*1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (OMR) m~--~~~~~~~~~~~~~--- (SUBR 01) OMB No. 2040-0004 A£1g_~ _p_JL!l.OX2.Q!XL _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ILII!_TE_~QEFJCE_Sil:~8:..SQ!\IL _ _ _ _ _ _ _ _ ~ 116 G j F - FINAL ___ 3M~M~W2~-------- DISCHARGE NUM!l_ER__ BACKWASH ~QU\'L _T'li\_o_SfllUQ\'AI:IJ>IUCI.f.A_RI'LANT______ _ r~qRING ~1~ - ~ ro]O 1 ~~ j,gQ)tiQ__ HAMJ!,T_QNJ;;QUr-ITY_ _ _ _ _ _ _ _ _ _ EFFLUENT x~~* I ~~ I ~; ATTN: stephanie A. Howard PARAMETER IX I _,. . ,. . __ ,-*-* - From 1 YU"I. I II I U'A. &.uru.ou*u 01 1 QUALITY OR CONCENTRATION NO DISCHARGE D ... N()TE' Read instructions before oompletino this form

                                                                                                                                                                                                                   . *--~---' EX I NO. IFREQUENCY OF I  SAMPLE TYPE I ... ,........                          I
                                                                                    ........                                                                                                                          UNITS ~

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                                                                                                                                                                             .. ----~--

I 1<******* .. - 0 1 I 30 I VISUAL I I ...........,., 84066 1 0 EFFLUENT GROSS VAL_UE 0 PERMIT REQUIREMENT SAMPLE I . .o ' REPORT

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REQUIREMENT l___ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Christopher R. Church 1Certify under penally of law that !his document and aU auachments were prepared under my direction or supervision in accordance with a system designed to assure that qualilted personnel properly galher and evaluate the information submitted. Based on my inquiry of the person or persons who manage tile system, or those persons directly responsible for gathering the information, the informalion submitted is , to the best of my knowledge and belief, true,

                                                                                                                                                                           '       ;;:::      ./;:

Se uoyah Site Vice President l___2E_~~fjO~E 423 843-7001 I I 10 I DATE 10 I 07 Sequoyah Site Vice President

                                                                                                                                                                                                                ~

accurate, and complete I am aware that there are significant p<malties for submillmg false SIGNATURE OF PRINCIPAL EXECUTIVE 1--------=-=-=--::::-::=::-:==-------jlnlormatron, including the possibility of f1ne and imprisonment lor knowing v1olations OFFICER OR AUTHORIZED AGENT NUMBER I YEAR I MO I DAY TYPED OR PRINTED

                 --~---**--*-*- *--****-*-*-                                                                                                                                                                             -

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (ReFerence all attachments her Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) PrevioiJs editions may be used *Page1of1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved t- - N~me __TVA- SEQUOYJ\H_NIJCLE~~ PLANT____ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Mi!:!!:~S _ _p.Jl.JlQl(_~QOO__ __________ . __ _t!NT~~QFF!C!;; _SQ-~A_~SQNl_ _______ -~ __ _ I TN002645o-l -Tf781 F- FINAL ________SQDQ.Y: .P.P.J~Y.. TN .J?N4__ - -- _ - --- -- __PE_RMIT NUMB-~ !l!SC.H_A_RQ~ NUMB~ILJ BACKWASH fidljty __T.VA.:_SEOIJOY_AH_NU_CLE_AR PlAJ'H

                                                                                                                                 ~          M       ~j RJNii..___

_ _:__=I EFFLUENT Tot~ Qfgtj lQQtiQ____H_A_M!LTQN.CQYro..!IV______________ .. ATTN: Stephanie A. Howard FromTQUo1J NO DISCHARGE [ _ ] *** NOTE- Read instructions before completlnq this form PARAMETER ~x</~ QUANTITY OR LOADING 1=~------~-~---o-uAL~TY~~R~~NCENT~-~TI(iN- -- -- - -- - r-~~---- ,---~-------- 1'110. FREQUENCY 1 SAMPLE EX OF TYPE

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PERMIT MEITffle PRINCIPAL EXECUTIVE OFFICER 1 Certify

      ---- - -         -~--------            ----

REQUIREMENT u~der penalty ol law that thiS document and all aHa~.:hmenls wem prepared under dlrec11on or superviSion m accordance w1ih a system deSigned lo assure that quahf1ed personnel my -- z;;1.( -c"/~---h-

                                                                                                                                                                                                                      ~
                                                                                                                                                                                                                              -              -] ----- ---

TELEPHONE- E D A T E

                                                                                                                                                                                                                                                                       --~      -- -            ~-----1
                                                                                                                                                                                                                                                  ----~                             J_-1--

Chnstopher R Church properly gat11er and evaluate the mlormat1on submJitecl Based on my InqUiry ol the person or persons who manage the system, or those persons directly responsible lorgattlenng the Sequo ah S e Vice Pr !dent

                                                            .nforrnai!On tile .nforllliliiOfl subnuUed 1s to th<J best of my knowledge and ballet true                                                 Y                                          423          843-7001           10        10     07 Sequoyah Site      Vtce President                  accurate and complete I am aware that there are significant penalt1es lor subrmtllng false                               -SIGNATUREOF PRINCIPAl EXEcUTIVE                           _             ___ _ _

L - _

   ---~~EO_~~~RINTE~-----

_ ~ _ COMMENTS AND EXPLANATION OF ANY VIOLATIONS 1ntonnat10n InclUding the possibility of line and 1mpnsonment tor knowing vtolahons

                                                                 ---------------~-

(Reference all attachments her OFFICER OR AUTHORIZED AGENT Ali'EA __c_ooe NUMBER YEAR MO DAY Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Jf MAJOR Form Approved l'l_a[!1_e___TIJA.:_ SEQUOYA_H NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 At;~Qre_s~ ___ P ,_Q._§OX4QQQ___ _ ~ _ -* ___ ~ *-- _____ -* ______ <Lf\!TERQfFJCU~cJ~c_SQNL _ _ _ .. SQDD.Y

  • PA!S.Y~ TN ~?~4- ____________ _

f~t.Y___T_'l.A.~_5EOY81i..f.IUC!..E.AR_elA_NT__ _ L p~~~~~~H~R 5 _D~:~iA~~~~£~j F- FINAL WASTEWATER & STORM WATER From§~~:[ ~~t!~r~:tro!i~~~~ _lQc_a_tjQ_ ___H_A_MJltOI\I__CQUNTY EFFLUENT NO DISCHARGE lxx] *** ATTN: Stephanie A. Howard NOTE: Read Instructions before completinA this farm

                                                                                                                                                                                                                                                                    ***-"I'"'**-

X PARAMETER QUANTITY OR lOADING QUALITY OR CONCENTRATION NO. AVERAGE

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L EX OF ANALYSIS TYPE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT

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19

                                                                             -- r-- **"';;;;;;----*                                                ........                                         ----          -------*-                              ~---

00300 1 0 0 PERMIT "**"***"~~ 2.0 **"~~***** ******""If MGIL TWICEf- GRAB REQUIREMENT EFFLUENT GROSS VALUE __ _l;lf\ILY MN WEEK SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 19 00530 1 0 0

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      -----~--------------

SOLIDS, SETTLEABLE SAMPLE ******** ********

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MEASUREMENT 25 f---pERMIT ___ -*- ........ ----- --'"**-*------ -----~~--- j-~-~-~~~-~ *----- -----~- 00545 1 0 0 ""*""*""**"" ""*"*"""** ***"'*"** "111r11:*1t:itl\" 1.0 MUL ONCE/ GRAB REQUIREMENT *. EFFLUENT GROSS VALUE DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 03

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MEASUREMENT TREATMENT PLANT 50050 1 0 0 PERMIT REQUIREMENT REPORT I** REPORT MGD c-------------...

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NAME/TiTi.E-PRi-N6PAl EXECunVE oFFICE.R ~-----*-* --

                                                                                                                                                 ----        ~--------

1-C-e~-ify ~d-~r*p~~lty of law th-a( ihi~-d~~--~;;.;t~-~d-~-~~-~tl~~hments were p.repared under my

                - - - - - - **--- -----~* -* -----**-- drrec:tron or super>"rsron 1n ar:c:ordall(:e wrlh a system desrgood to assure that quail fred personnel                      c___
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                                                                                                                                                                                                                                                  -~~~~-- ----- ---* DATE TELEPHONE
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                                                                                                                                                                                  - .. ~-*~o_~*~- ~'-~-*- ~1.
                                                                                                                                                                                          ?                     /'/-;
                                                                                                                                                                               -----~-~uoyah i                  Christopher R. Church                        properly gather .and e ..aluale lhe mformallon subnulted_ Based on my 1nqurry of the per~on or 1                                                               perSO(lS who manage the syslem, or lho5e persons directly responsible lor galherrngthe rniOflnallon, the Information submitted IS, to the best of my knowledge and belief, true,                                            _Site       e    restZ:t       __     423         843-7001             10       10      07 Sequoyah Site Vice President                        accrxale, and complete 1am aware that lhere are significant penallies for submittrng false                      SIGNATURE       OF    PRINCIPAl EXECUTIVE               _ __     _ ___        .    . __      __            _____ _

_ ___ --*- ------*~-"-----*-~--*--*-- informatron. includrng the possibrhty of frne and rmprisoomenllur knowing violatrons TYPED OR PRINTED COMMENTS AND EXPlANATION OF ANY VIOLATIONS fl?eference all attachments her During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

                      --*--------*--*---     --~-----.------*-

EPA Form 3320-1 {REV 319!3) Previous editions may be osed Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- September 2010 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Bethany Hyatte DATE: 1016110 CONCURRENCES Name R c Signature - Comment Date v N S. A Howard X cJt!~U2d~ Q;_ ~0-A ~. ~ujii!D G.J. Wynn X ( \- L2\./ 1 0/1/lc B. A Wetzel K. Langdon X X {"J kL

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X~ n~~ C. R Church l<7f{cJ i o F ' L INSTRUCTIONS: Originator will determine the review/concurrence assignment REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 101110 801- NPDES CORRESPONDENCE November 10, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR OCTOBER 2010 Enclosed is the October 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Daily sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 10/26/2010 have all yielded results below detection limits for oil and grease. On 10/1/2010 the permit limit for pH at Outfall103 (discharge from the Low Volume Waste Treatment Pond- LVWTP) was exceeded. The pH sampled at 1800 EST on 10/1/2010 was 9.2. The permit limit is 6.0 to 9.0. A sample of Outfall1 01 (Diffuser Pond Discharge to the Tennessee River) was collected at 2116 EST to verify discharge to the river did not exceed limits. The pH of Outfall 101 was within limits at 7.9 pH units. On 10/1/2010 at 2300 EST the pH of Outfall 103 was 9.0 and determined to be back within permit limits. The suspected cause of the exceedances at Outfall 103 was a high pH discharge from the High Crud Tank of the condensate demineralizer system. The pH of the High Crud Tank was approximately 9.0 at the time of discharge to the Turbine Building Sump which discharges to the Low Volume Waste Treatment Pond. The event was documented in the site's corrective action program. Corrective action to date includes the revision of the condensate demineralizer effluent procedure to change the acceptable effluent pH range from 6.0 to 9.0 to 6.0 to 8.0. A study has also been initiated to determine if an engineered pH control system to the LVWTP is a viable option. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.

I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. ~el~//_ ~-~' Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control ATIN: Document Control Desk State Office Building, Suite 550 Washington, D.C. 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc: B. E. Brickhouse, LP 5U-C A. A. Ray, WT 11A-K C. R. Church, OPS 4A-SQN G. R. Signer, WT 6A-K S. A . Howard, OPS 5N-SQN B. A. Wetzel, OPS 4A-SQN K. Langdon, POB 2B-SQN G. J. Wynn, POB 2B-SQN D. B. Nida, LP 5U-C K. M. Hodges (EDMS), LP 2V-C

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 November 10, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR OCTOBER 2010 Enclosed is the October 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Daily sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 10/26/2010 have all yielded results below detection limits for oil and grease. On 10/1/2010 the permit limit for pH at Outfall103 (discharge from the Low Volume Waste Treatment Pond- LVWTP) was exceeded. The pH sampled at 1800 EST on 10/1/2010 was 9.2. The permit limit is 6.0 to 9.0. A sample of Outfall 101 (Diffuser Pond Discharge to the Tennessee River) was collected at 2116 EST to verify discharge to the river did not exceed limits. The pH of Outfall101 was within limits at 7.9 pH units. On 10/1/2010 at 2300 EST the pH of Outfall103 was 9.0 and determined to be back within permit limits. The suspected cause of the exceedances at Outfall 103 was a high pH discharge from the High Crud Tank of the condensate demineralizer system. The pH of the High Crud Tank was approximately 9.0 at the time of discharge to the Turbine Building Sump which discharges to the Low Volume Waste Treatment Pond. The event was documented in the site's corrective action program. Corrective action to date includes the revision of the condensate demineralizer effluent procedure to change the acceptable effluent pH range from 6.0 to 9.0 to 6.0 to 8.0. A study has also been initiated to determine if an engineered pH control system to the LVWTP is a viable option. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.

I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Since~y, .

    /,.A~~

~herR. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control ATTN: Document Control Desk State Office Building, Suite 550 Washington, D.C. 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) Nam~-~~SEOUOYA~UCLEARP~~--- OMB No. 2040-0004 Address_?.O.JtOX 20012_ _ _ _ _ _ _ _ _ _ _ _ ---~INmOFFICESB*2A*SQ~------- ---~@DY~AISY.TN37~--------

                                                                                                    ~     *- *---*-i TN0026450 PERMIT NUMBER
                                                                                                                                              ~            101 G j)iscHARGE NUMBER        I (SUBR  01)

F- FINAL DIFFUSER DISCHARGE r:¥J"'"" r=i Faclli~-~~EQUO~HNUCLEARP~NT _ _ _ _ _ Locatio~A~WNCOUNIT_ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN: Stephanie A. Howard From I".. I 10 10 MO 01 To 10 10 MO 31 I I PAY NO DISCHARGE D *** NOTE: Read instructions before comp\etin!l this form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 31 I 31 MODELD MEASUREMENT ** 25.6 04 0 CENTIGRADE 00010 z 0 0 PERMIT ******** ******** **** ******** ******** 30.5 DEG.C. SEE CKREQ REQUIREMENT INSTREAM MONITORING DAILYMX PERMIT TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 35.7 31131 RCORDR MEASUREMENT ** 04 0 CENTIGRADE 00010 1 0 0 PERMIT ******** ******** **** ******** ******** REPORT DEG.C. SEE CKREQ REQUIREMENT EFFLUENT GROSS VALUE _DAILYMX PERMIT TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** 2.3 0 31 I 31 CALCTD MEASUREMENT ** 04 UPSTRM DEG.C 00016 1 s 0 PERMIT ******** ******** **** ******** ******** 3.0 DEG.C. CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE DAILYMX uous PH SAMPLE ******** ******** 7.7 ******** 8.1 0 5131 GRAB MEASUREMENT ** 12 00400 1 0 0 PERMIT ******** ******** **** 6.0 ******** 9.0 su WEEKLY GRAB REQUIREMENT EFFLUENT GROSS VALUE MINIMUM MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** 6 6 0 1 I 31 GRAB MEASUREMENT ** 19 00530 1 0 0 PERMIT ******** ******** **** ******** 30 100 MG/L MONTHLY GRAB REQUIREMENT EFFLUENT GROSS VALUE MOAVC DAILYMX OIL AND GREASE SAMPLE ******** ******** ******** <5 <5 0 1 I 31 GRAB MEASUREMENT ** 19 00556 1 0 0 PERMIT ******** ******** **** ******** 15 20 MG/L MONTHLY GRAB REQUIREMENT EFFLUENT GROSS VALUE MOAVC DAILYMX FLOW, IN CONDUIT OR THRU SAMPLE ******** 1633 ******** ******** ******** 0 31 I 31 RCORDR TREATMENT PLANT MEASUREMENT 03 ** 50050 1 0 0 PERMIT REQUIREMENT

                                                                         ********                  REPORT                 MGD               ********           ********          ********
                                                                                                                                                                                                     ....             CONTIN         RCORDR EFFLUENT GROSS VALUE                                                                              DAILYMX                                                                                                               uous
                                                                                                                                                         ?;;4 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER         1Certify under penalty of law that this document and all attachments were prepared under my                                                        TELEPHONE                     DATE direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church                properly gather and evaluate the infonnation submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President infonnation, the infonnation submitted is , to the best of my knowledge and belief, true,                                                     423        843-7001         10       11     08 Sequoyah Site Vice President              accurate, and complete. I am aware that there are sign~icant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.

SIGNATURE OF PRINCIPAL EXECUTIVE J OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY ITPED OR-PRINTED

                        --   ----    -       -'--      -         -                                     *--      --       --        -*      -                                                  CODE COMMENTS AND     EXP~NATION   OF ANY VIO~TIONS            fReference all attachments he!

No closed mode operation. The following infonnation is included in an attachment: 1. CCW data 2. veliger monitoring data EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 2

DMR Attachment CCWData CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained. CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 10/13/2010@ 1627 <0.10 mg/1 10/15/2010@ 0549 JOB EPH

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Temp. ("C) Sample Date Asiatic LOCATION LOCATION Gravid Asiatic COLLECTED BY ZM1m3 Temp. ("C) Clamslm3 Clam 11/03/2009 133 0 16 11/03/2009 76 16 In plant RCW CMW 11/10/2009 417 6.1 16 11/10/2009 25 16 In plant RCW 'CMW 11/17/2009 269 0 16 11/17/2009 0 16 In plant RCW CMW 11/24/2009 36 50 15 11/24/2009 18 15 In plant RCW CMW 12/01/2009 32 0 13.5 12/01/2009 0 13.5 In plant RCW WE 12/08/2009 38 0 11 12/08/2009 0 11 In plant RCW CMW 01/05/2010 0 0 6 01/05/2010 0 6 In plant RCW B 01/12/2010 0 0 5 01/12/2010 0 5 In plant RCW 01/19/2010 0 0 6 01/19/2010 0 6 In plant RCW p 01/26/2010 32 0 7.5 01/26/2009 0 7.5 In plant RCW NRT 02/02/2010 0 0 7 02/02/2010 0 7 In plant RCW MSWIWDT 02/09/2010 0 0 8 02/09/2010 0 8 In plant RCW BLITC 02/16/2010 0 0 5 02/16/2010 0 5 In plant RCW BJ 02/23/2010 11.7 0 7 02/23/2010 0 7 In plant RCW BJ 03/02/2010 0 0 6 03/02/2010 0 6 lnplant RCW PB 03/09/2010 0 0 8 03/09/2010 0 s* In plant RCW MJW 03/16/2010 0 0 10 03/16/2010 0 10 In plant RCW BC 03/23/2010 14 0 11 03/23/2010 0 11 In plant RCW BC 03/30/2010 0 0 14 03/30/2010 0 14 In plant RCW BAPO Apr-2010 no samples collected May-2010 no samples collected Jun-2010 no samples collected Jul-2010 no samples collected Aug-2010 no samples collected Sep-2010 no samples collected Oct-2010 no samples collected

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (OMR) Nam~-N~SEOUOYA~UCLEARP~~---

                                                                                                                                - -\1- -                                   (SUBR 01)                               OMB No. 2040-0004 Address_p.o._e_ox 2000_ _ _ _ _ _ _ _ _ _ _ _

---~INTEROFFICESB-2A-S~L _ _ _ _ _ _ _ ~-- TN0026450 101 G -~ F -FINAL ---~ODDY~AI~TN3738L _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE flCili~-~~EQUO~HN~LE~PLANL _ _ _ _ I.... I r~r*c r~t 10 I 31 I Locatlo~AMU~COUNTI_ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN: Stephanie A. Howard From 10 MD 10 01 To 10 MO OAV *** NO DISCHARGE D *** NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** 0.018 0.028 27/31 GRAB MEASUREMENT ** 19 0 50060 1 0 0 PERMIT ******** ******** **** ******** 0.10 0.10 MG/L WEEK- CALCTD REQUIREMENT EFFLUENT GROSS VALUE MOAVG INSTMAX DAYS ~TEMPERATURE - C, RATE OF SAMPLE ******** 0 ******** ******** 31/31 CALCTD MEASUREMENT 62 ** 0 CHANGE 82234 1 0 0 PERMIT REQUIREMENT

                                                                         ********                         2                DEG              ********            ********           *******             ....              CONTIN        CALCTD EFFLUENT GROSS VALUE                                                                               DAILYMX                  C/HR                                                                                           uous SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER          I Certify under penalty of law that this document and all attachments were prepared under my            .      :,...-  .--?Y-:::;                    TELEPHONE                    DATE
                                                                                                                                                         ~ft.-~

direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice Pre 1dent information, the information submitted is , to the best of my knowledge and belief, true, 423 I 843-7001 10 11 08 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TIPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE 1 NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments her The following injection occurred: Floguard MS6236 (max. calc. cone. was 0.059mg/L-Iimit 0.2mg/L) .. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (OMRI Nam~-W~SEOUO~~UCLEARP~~--- (SUBR 01) OMB No. 2040-0004 Address__?.O.__!!.OX 2000_ _ _ _ _ _ _ _ _ _ _ _ ---~INTEROF~~B-2A-SQ~------- TN0026450 ~ 101 T I F -FINAL ---~ODDY~AI~TN2~-------- P RMIT NUMBER ~SCHAROE NUMBER BIOMONITORING FOR OUTFALL 101 Facili~-~~EQU~A~UCLE~PLANL _ _ _ _ Loc~io~A~WNCOUNIT_ _ _ _ _ _ _ _ _ _ ATIN: stephanie A. Howard From l>***i 10 MO 10 rwrG Fl 01 To 10 MO 10 I O& 31 I EFFLUENT

                                                                                                                                                                           *** NO DISCHARGE           D       ***

NOTE: Read instructions before completing this form X PARAMETER QUANTITY OR LOADING QUAliTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ******** ******** Other ******** ******** MEASUREMENT ** 23 CERIODAPHNIA TRP38 1 0 0 PERMIT ******** ******** **** 45.2 ******** ******** PERCENT SEE COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM PERMIT IC25 STATRE 7DAY CHR SAMPLE ******** ******** Other ******** ******** MEASUREMENT ** 23 PIMEPHALES TRP6C 1 0 0 PERMIT ******** ******** **** 45.2 ******** ******** PERCENT SEE COMPOS REQUIREMENT EFFLUENT GROSS VALUE MIMINUM PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

                                                                                                                                                          ~&;g ~

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel TELEPHONE DATE I Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the sequoyah siZce Pres*dent Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE 423 l 843-7001 10 11 ool TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments her Toxicity sampling began on October 31 and ended on November 5. The toxicity report will be included with the November 2010 DMR. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilitv Name/location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR. DISCHARGE MONITORING REPORT (DMRJ NamL_N~SEOU~A~UCLEARP~~--- OMB No. 2040-0004 Address _p.o ....!i_OX 2000_ _ _ _ _ _ _ _ _ _ _ _ ---~INTER~FICESB-2A-SQ~------- ---~ODDY~AISY.TN3738L ______ _ 1- ---*-1 I TN0026450 PERMIT NUMBER 103 G DISCHARGE NUMBER I (SUBR 01) F- FINAL LOW VOL. WASTE TREATMENT POND Faciii~-~~~UO~~UCLEARPLANL _ _ _ _ LOCM~~AMINCOUNIT_ _ _ _ _ _ _ _ _ _ ATTN: stephanie A. Howard From I~: I : rwrr~: ri1 ~g I"tf I EFFLUENT

                                                                                                                                                                           *** NO DISCHARGE            D      ***

NOTE: Read instructions before completinR.. this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ******** 6.6 ******** 25 I 31 MEASUREMENT

                                                                           ********                                          **                                                       9.3              12        1                      GRAB 00400     1       0     0                          PERMIT                   ********                   ********                **                6.0             ********              9.0              su              THREE!           GRAB REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                         MINIMUM                             MAXIMUM                                  WEEK SOLIDS, TOTAL SUSPENDED                           SAMPLE MEASUREMENT 120                        176                 26               ********             12                  17              19        0       4131           GRAB 00530     1                                        PERMIT                      380                       1250 0     0 REQUIREMENT LBS/DY              ********             30                 100             MG/L             WEEKLY           GRAB EFFLUENT GROSS VALUE                                                      MOAVC                     DAILYMX                                                     MOAVC             DAILYMX OIL AND GREASE                                    SAMPLE                        <58                       <79                 26               ********             <6                  <7              19        0        4131          GRAB MEASUREMENT                                                                                                                                                                                       I I

00556 1 0 0 PERMIT REQUIREMENT 190 250 LBS/DY ******** 15 20 MG/l WEEKLY GRAB I EFFLUENT GROSS VALUE MOAVC DAILYMX MOAVC DAILYMX FLOW, IN CONDUIT OR THRU SAMPLE MEASUREMENT 1.121 1.759 03 ******** ******** ******** ** 0 31 I 31 TOTALZ TREATMENT PLANT 50050 1 0 0 PERMIT REQUIREMENT REPORT REPORT MGD ******** ******** ******** .. SEE TOTALZ EFFLUENT GROSS VALUE MOAVC DAILYMX PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law thai this document and all attachments were prepared under my direction or supervision in accordance wilh a system designed to assure that qualified personnel 4/fif

                                                                                                                                                                  ./"
                                                                                                                                                                                     --               TELEPHONE                      DATE Presi~

Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering lhe Sequoyah Site Vice information, the information submitted is , to the best of my knowledge and belief, true, 423 I 843-7001 10 11 08 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY ITPED OR PRINTED -- - ----- CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS fReference all attachments he! On 10/01/2010 pH exceeded 9.0 limit. See cover letter for additional information. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Fonn Approved. DISCHARGE MONITORING REPORT (DMR) Nam~-N~SEOUO~~UCLEARP~~--- (SUBR 01) OMB No. 2040-0004 Mdress_F.O.J!..OX 2000_ _ _ _ _ _ _ _ _ _ _ _ ---~INTEROFFICESB-2A-SQ~------- TN0026450 ~~*-*-~ 107 G F- FINAL ---~ODDY~AI~TN2~L ______ _ I PERMIT NUMBER j)JSCHARGE NUMB METAL CLEANING WASTE POND r4*'*G r~l flCIIi~-~~EQ@~~UCLE~PLAN~---- LOC~io~A~TONCOUNIT _________ _ EFFLUENT ATIN: Stephanie A. Howard From I 10 I 10 YEA* MO 01 To 10 MD 10 I 31 I OAY NO DISCHARGE IXX I *** NOTE: Read instructions before completing this form. X PARAMETER OUANTrrY OR LOADINO OUALIIT OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS jPH SAMPLE ******** ******** ******** i MEASUREMENT ** 12 00400 1 0 0 PERMIT ******** ******** **** 6.0 ******** 9.0 su DAILY GRAB REQUIREMENT EFFLUENT GROSS VALUE MINIMUM MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** ******** MEASUREMENT ** 19 00530 1 0 0 PERMIT ******** ******** **** ******** ******** 30 MG/L DAILY COMPOS REQUIREMENT EFFLUENT GROSS VALUE DAILYMX OIL AND GREASE SAMPLE ******** ******** ******** ******** MEASUREMENT ** 19 00556 1 0 0 PERMIT ******** ******** **** ******** ******** 15 MG/L DAILY GRAB REQUIREMENT EFFLUENT GROSS VALUE DAILYMX PHOSPHORUS! TOTAL (AS P) SAMPLE ******** ******** ******** ******** MEASUREMENT ** 19 00665 1 0 0 PERMIT ******** ******** **** ******** ******** 1.0 MG/L DAILY COMPOS REQUIREMENT EFFLUENT GROSS VALUE DAILYMX COPPER, TOTAL (AS CU) SAMPLE ******** ******** ******** ******** MEASUREMENT ** 19 01042 1 0 0 PERMIT

                                                                                      ********                   ********              ****              ********           ********             1.0             MG/L              DAILY        COMPOS REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                                                                       DAILYMX IRON, TOTAL (AS FE)                                        SAMPLE                    ********                   ********                                ********           ********

MEASUREMENT ** 19 01045 1 0 0 PERMIT ******** ******** **** ******** ******** 1.0 MG/L DAILY COMPOS REQUIREMENT EFFLUENT GROSS VALUE DAILYMX FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** MEASUREMENT 03 ** TREATMENT PLANT 50050 1 0 0 PERMIT REPORT REPORT MGD ******** ******** ******** .... DAILY CALCTD REQUIREMENT EFFLUENT GROSS VALUE MOAVG DAILYMX ... - NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, 423 843-7001 10 11 08 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE 1 - - - - - - - - - - - - - - - - - - - - - - ! i n f o r m a t i o n . including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY ITPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS fReference all attachments he! No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) Nam~-~~SEOU~A~UCLEARP~~--- OMB No. 2040-0004 Address _p..Q.__!i_OX 200Q_ _ _ _ _ _ _ _ _ _ _ _ ---~INTEROFFICESB-2A-SQ~------- ---~ODDY~AISY.TN37~-------- F TN0026450 -~~~--~ PERMIT NUMBER A 11 0 G CHARGE NUMBER (SUBR 01) F- FINAL RECYCLED COOLING WATER Facili~-~~EQ@YA~~LEARPLAN~--- Fromh~*l ~g mr~:nrl ~g I";; I EFFLUENT Loc~o~AMI~~OUN~---------- NO DISCHARGE IXX I *** ATTN: stephanie A. Howard NOTE: Read instructions before completing this form. C>< PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM UNITS ANALYSIS AVERAGE MAXIMUM TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** MEASUREMENT 04 04 CENTIGRADE 00010 z 0 0 PERMIT ******** ******** DEGC ******** ******** 38.3 DEGC DAILY GRAB-4 REQUIREMENT INSTREAM MONITORING DAILYMX PH SAMPLE MEASUREMENT PERMIT 12 su 00400 1 0 0 REQUIREMENT

                                                                          ********                                          ****                 6.0                                          9.0                        WEEKLY           GRAB EFFLUENT GROSS VALUE                                                                                                                       MINIMUM                                  MAXIMUM SOLIDS, TOTAL SUSPENDED                           SAMPLE MEASUREMENT PERMIT
                                                                                                                             -                ********            ********                                19 00530      1      0    0 REQUIREMENT
                                                                                                                            ****              ********            ********                    30         MG/L              DAILY        COMPOS EFFLUENT GROSS VALUE                                                                                                                                                               DAILYMX OIL AND GREASE                                    SAMPLE MEASUREMENT
                                                                                                                             -                ********            ********                                19 00556     1      0 EFFLUENT GROSS VALUE 0                          PERMIT REQUIREMENT DAILYMX 15         MGIL              DAILY          GRAB FLOW, IN CONDUIT OR THRU                         SAMPLE                                                                                       ********            ********            ********

MEASUREMENT 03 ** TREATMENT PLANT 50050 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT REPORT MOAVG REPORT DAILYMX MGD ******** ******** ******** - DAILY CALCTD CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 19 50060 1 0 0 PERMIT REQUIREMENT

                                                                          ********                    ********              ****                                                            0.10         MG/L             WEEKLY         GRAB-4 EFFLUENT GROSS VALUE                                                                                                                                                                DAILYMX SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Christopher R. Church I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or
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                      *   .       .               information, the information submitted is, to the best of my knowledge and belief, true,                                                          423       843-7001        10        11     08 Sequoyah S1te V1ce President

'---------~PED OR PRINTED. accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA COOE I f...----+-1-----+--1---+--- NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments her No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) Nam~-W~SEOUO~~UCLEARP~~--- (SUBR 01) OMB No. 2040-0004 Address_p.O.JtOX 2.000_ _ _ _ _ _ _ _ _ _ _ _ ---~INTEROFFIC~B-2A*S~L ---~ODDY~AI~.TN3738L _ _ _ _ _ _ _ ______ _ F TN0026450 PERMIT NUMBER I~ A 110 T CHARGE NUMBER I F- FINAL RECYCLED COOLING WATER Facili~-~~EQ~~HNUCLEARP~N~---- I'1: I ~ r~r~: P!fj ~ I w ~catio~AMIOONCOUNIT _________ _ EFFLUENT ATTN: Stephanie A. Howard From I NO DISCHARGE IXX I *** NOTE: Read instructions before completin!l this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE I EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ******** CERIODAPHNIA MEASUREMENT ** 23 TRP3B 1 0 0 PERMIT ******** ******** **** 45.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ******** PIMEPHALES MEASUREMENT ** 23 TRP6C 1 0 0 PERMIT ******** ******** **** 45.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TinE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my Christopher R. Church direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true,

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Sequoyah Site Vice Presid nt 423 TELEPHONE 843-7001 10 DATE 11 08 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY TYPED OR PRINTED COOE

                                                                                                                                                                                       ~-~

COMMENTS AND EXPLANATION OF ANY VIOLATIONS rReference all attachments he! No Discharge this Period EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) NamL_~~SEOUOYA~UCLEARP~~--- (SUBR 01) OMB No. 2040-0004 Address _F.O.JtOX 200Q_ _ _ _ _ _ _ _ _ _ _ _ ---~INTEROF~UB-2A*S~L _ _ _ _ _ _ _ ~ - 116 G~ F- FINAL ---~ODDY~AI~.TN2~-------- QSCHARGE NUMBER BACKWASH Facili~-~~EQ~~~UCLE~PLANL ___ _ l~o* i 7g rwr~: L~l ~g I~ I Locmio~AMI~~OUNIT_ _ _ _ _ _ _ _ _ _ EFFLUENT ATIN: stephanie A. Howard From NO DISCHARGE D *** NOTE: Read instructions before completinQ this form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM .UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE MEASUREMENT

                                                                                                                           -               ********            ********           0                9A         0       1 I 31       VISUAL 01345    1    0    0                            PERMIT                  ********                    ********              ****             ********            ********       REPORT             PASS=O                SEE         VISUAL REQUIREMENT                                                                                                                                           FAIL=1 EFFLUENT GROSS VALUE                                                                                                                                                         MOTOTAL                                 PERMIT OIL AND GREASE VISUAL                          SAMPLE                    ********                        0                                  ********           ********        ********                               1 I 31       VISUAL MEASUREMENT                                                                    94                                                                       **        0

'84066 1 0 0 EFFLUENT GROSS VALUE PERMIT REQUIREMENT

                                                                         ********                 REPORT MOTOTAL YES=1 NO=O
                                                                                                                                           ********            ********        ********            --                  SEE PERMIT VISUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT I

SAMPLE I MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, 423 843-7001 10 11 08 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY ITPED OR PRINTED -~ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments her Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ Form Approved. MAJOR NamL_W~SEOUOYA~UCLEARPLA~--- DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address_.P.O.J!_OX 2000_ _ _ _ _ _ _ _ _ _ _ _

                                                                                                                                              ~-117---~          G      F- FINAL

---~INTER~FICESB*2A*SQ~------- ---~ODDY~AI~TN3738~------- DISCHARGE.NUMBER BACKWASH Facili~-~~EQUOYA~UCLEARP~NL _ _ _ _ Froml~l itij"'~:r;:J ~g IWI ~c~io~AMI~NCOUNIT_ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN: stephanie A. Howard NO DISCHARGE D ... NOTE: Read instructions before completing this form PARAMETER DEBRIS, FLOATING (SEVERITY) 01345 1 0 0

                                        ><     SAMPLE MEASUREMENT PERMIT AVERAGE QUANTITY OR LOADING MAXIMUM UNITS MINIMUM QUALITY OR CONCENTRATION AVERAGE MAXIMUM 0

REPORT UNITS 9A PASS=O NO. EX 0 FREQUENCY OF ANALYSIS 1 I 31 SEE SAMPLE TYPE VISUAL VISUAL REQUIREMENT FAIL=1 EFFLUENT GROSS VALUE MOTOTAL PERMIT OIL AND GREASE VISUAL SAMPLE ******** 0 ******** ******** ******** 1 I 31 VISUAL MEASUREMENT 94 ** 0 84066 1 0 0 PERMIT REQUIREMENT

                                                                         ********                 REPORT                YES=1               ********          ********         ********            ....               SEE         VISUAL, NO=O EFFLUENT GROSS VALUE                                                                              MOTOTAL                                                                                                            PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Christopher R. Church I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the infonnation submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the infonnation, the infonnation submitted is , to the best of my knowledge and belief, true, Z              -z.~

Sequoy::Sit?vfce Pres~ 423 TELEPHONE 843-7001 10 DATE 11 08 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false I ITPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA COllE_ 1 NUMBER YEAR MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments he! Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ Form Approved. MAJOR NamL_N~SEOUO~~UCLEARP~~--- DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address_F.O._!l_OX 2000_ _ _ _ _ _ _ _ _ _ _ _

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---~INmOFFICESB-2A-SQ~------- TN0026450 118 G F- FINAL ---~DY~AI~TN3738L ______ _ PERMIT NUMBER I DISCHARGE NUMBER WASTEWATER & STORM WATER Facili~-~~EQUO~HNUCLEARPLANL _ _ _ _ Locatio~A~WNCOUNTI_ _ _ _ _ _ _ _ _ _ ATTN: Stephanie A. Howard From In**i 10 MO 10 r~*ING 01 To rt10 MO 10 I IPAX 31 EFFLUENT NO DISCHARGE IXX I *** NOTE: Read instructions before completinQ this form X PARAMETER OUANTrrY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE ******** ******** ******** ******** MEASUREMENT ** 19 00300 1 0 0 PERMIT ******** ******** **** 2.0 ******** ******** MG/L TWICE! GRAB REQUIREMENT EFFLUENT GROSS VALUE DAILYMN WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** ******** MEASUREMENT ** 19 00530 1 0 0 PERMIT ******** ******** **** ******** ******** 100 MG/L TWICE/ GRAB REQUIREMENT EFFLUENT GROSS VALUE DAILYMX WEEK SOLIDS, SETTLEABLE SAMPLE MEASUREMENT

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  • ONCE! ESTIMA REQUIREMENT EFFLUENT GROSS VALUE MOAVO DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE I Christopher R. Church direction or supervision in accordance wilh a system designed lo assure that qualified personnel properly gather and evaluate the informalion submitted. Based on my inquiry of the person or ~ ?I __. . persons who manage the system, or those persons directly responsible for gathering the information. the information submitted is , lo the best of my knowledge and belief, true, Sequoyah Site Vice Presi~ 423 I 843-7001 10 11 08 Sequoyah Site Vice President accurate, and complete. I am aware thai there are significant penallies for submitting false SIGNATURE OF PRINCIPAt EXECUTIVE informalion, including lhe possibilily of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY I TYPED OR PRINTED CODE_ - COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments he! During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PlANT- October DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Bethany Hyatte DATE: 11/8/10 CONCURRENCES Name R C Signature - Comment Date V N S. A Howard X G. Jimmy Wynn X B. A Wetzel X K. Langdon X C. R. Church X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 101209 800- NPDES CORRESPONDENCE December 9, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR NOVEMBER 2010 Enclosed is the November 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Also, enclosed is the revision to the Discharge Number 101 T, Biomonitoring for Outfall 101 for October 2010. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 11/29/2010 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, r1 t;~, Michael D. Ska Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control ATTN: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc: B. E. Brickhouse, LP 5U-C A. A. Ray, WT 11A-K D. A. Day, POB 2B-SQN G. R. Signer, WT 6A-K S. A. Howard, OPS 5N-SQN M. D. Skaggs, OPS 4A-SQN K. Langdon, POB 2B-SQN B. A. Wetzel, OPS 4A-SQN D. B. Nida, LP 5U-C K. M. Hodges (EDMS), LP 2V-C

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (OMR) m~--N~ SEOUOYA~UCLEARP~~ --- (SUBR 01) OMB No. 2040-0004 Ad!ttess _P_,Q._!i_OX2J!!XL _ _ _ _ _ _ _ _ _ _ _ - --~lmER~~UB-2A - SO~ ------- ---~OD~~M~W3~- ---- --- I TN0026450 PERMIT NUMBER

                                                                                                                                                \ \            101 T DISCHARGE NUMBER
                                                                                                                                                                           \

F- FINAL BIOMONITORING FOR OUTFALL 101 ~~~-~~~~~~~lE~P~ID____ _ 10 I 10 FJ"'"" To Fl EFFLUENT Loc~~~AMIU~~~~--- ---- - - - ATTN: stephanie A. Howard From IYfAR MO 01 10 MO 31 I 10 I ""'

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NOTE: Read instructions before completin!l this form.

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PARAMETER OUANTrrY OR LOADING QUALrrY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS I C25 STATRE 7DAY CHR C ERIODAPHNIA SAMPLE MEASUREMENT

                                                                             ********                  ********                 ...             >100.0             ********          ********            23         0      1/180        COMPOS ITRP3B      1    0     0                            PERMIT
                                                                                                                               .....              45.2             ********          ********        PERCENT                 SEE        COMPOS REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                          MINIMUM                                                                     PERMIT IC25 STATRE 7DAY CHR                              SAMPLE                     ********                  ********                 ...              >100.0            ********          ********            23         0      1/180        COMPOS PIMEPHALES TRP6C      1     0    0 MEASUREMENT PERMIT REQUIREMENT
                                                                                                                               ....               45.2             ********          ........         PERCENT                SEE        COMPOS EFFLUENT GROSS VALUE                                                                                                                          MIMINUM                                                                     PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
                                                                                                                                                          ~"l~~,;deol NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penalty or law that this document and all allachments were prepared under my                                                                  TELEPHONE                     DATE direction Of supervision In I!CCOfdance wllh a system designed to assute that qualifoed personnel Michael D . Skaggs                    prope~y gather and evaluate the information submitted. Based on my inquiry or the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate,                                            423       843-7001          10      12     08 Sequoyah Site Vice President                and co.-nplete I am aware lhatlhere are significant penalties fo.- submilling false information.      SIGNATURE OF PRINCIPAL EXECUTIVE                I TYPED OR PRINTED including the possibility of fine and imprisonment fo.- knowing violations OFFICER OR AUTHORIZED AGENT           AREA CODF l    NUMBER         YEAR      MO    DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                 (Reference all attiJchments here Toxicity sampling began on October 31 and ended on November 5. This is the revised October 2010 DMR for 101T. Toxicity report is included.

EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved . MAJOR DISCHARGE MONITORING REPORT (DMR) Nam~_N~SEOU~A~UCLEARP~~ -- - (SUBR 01) OMB No 2040-0004 Address _p~ ....!!_OX 2000_ _ _ _ _ _ _ _ _ _ _ _ - - - ~INT~~~~~A-SQ~ - --- --- - - - ~W~~AI~ffl~3~- - - - --- - Faclli~_NA~~~ffi~UCLE~~NT _ _ _ _ _ E-=~: s~R JI 5 DISCHARG: ~~M~ER I F- FINAL DIFFUSER DISCHARGE r~rNGFt LoC~io~A~TONCNIT_ ___ ___ __ _ EFFLUENT ATIN: stephanie A. Howard From lx*10.. l MQ 11 01 To 10 MQ 11 1 ... 30 1 *** NO DISCHARGE D ... NOTE: Read instructions before completinll this form X PARAMETER OUANTrrY OR LOADING OUALrrv OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS T EMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 20.7 0 30 I 30 MODELD CENTIGRADE MEASUREMENT ** 04 00010 z 0 0 PERMIT

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                                                                           ********                  ********                   ..              ~*******           ********             32.9               04        0      30 I 30      RCORDR CENTIGRADE 00010      1     0 EFFLUENT GROSS VALUE 0                          PERMIT REQUIREMENT
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                                                                           ********                  ********                   ..              ********           ********              3.0               04        0      30 I 30       CALCTD UPSTRM DEG.C 00016      1     w     0                          PERMIT REQUIREMENT
                                                                                                                             ....               ********           ********              5.0            DEG.C.             CO NTIN        CALCTD EFFLUENT GROSS VALUE PH                                               SAMPLE MEASUREMENT
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                                                                           ********                  ********                    ..             ********                5                 5                19        0       1 I 30        GRAB 00530       1    0     0                          PERMrr REQUIREMENT
                                                                           ********                  ********                 ....              ********               30                100              MG/L           MONTHLY           GRAB EFFLUENT GROSS VALUE                                                                                                                                              MOAVO              DAILYMX OIL AND GREASE                                    SAMPLE                    ********                  ********
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()0556 1 0 0 PERMIT REQUIREMENT

                                                                           ********                  ********                 ....              ********               15                 20              MG/L           MONTHLY           GRAB EFFLUENT GROSS VALUE                                                                                                                                              MOAVC              DAILYMX FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT
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                                                                           ********                REPORT                   MGD                 ********            ********           ********
                                                                                                                                                                                                           ....            CONTIN         RCORDR EFFLUENT GROSS VALUE                                                                               DAILYMX                                                                                                                   uous (t~~~CZ:,;oem NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penally of law lhal this document and all attachments were prepared under my                                                                    TELEPHONE                     DATE direction or supervision In accordance With a *Ystem destgned to assuru lha1 qualified personnel Michael D. Skaggs                    properly gather and evaluate lhe information submitted. Based on my inquiry of the person or persons who manage the syslem, or those persons directly responsible for galhering lhe 1nformation, the infonnation submitted is , to the best of my knowledge and belief, lrue, accurate,                                                423        843-7001        10       12     08 Sequoyah Site Vice President              ~< nd complete I am aware that there are significant penal lies for submilling false infonnation, Including the possibility or fine and imprisonment for knowing violations SIGNATURE OF PRINCIPAL EXECUTIVE                  I
                                                                                                                                                                                                    ~

OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (f?eference all attachments flere No closed mode operation . The following information is included in an attachment: 1. CCW data 2. veliger monitoring data 3. toxicity report EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2

DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained. CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 11/09/2010@ 1030 <0.10 mg/1 11/12/2010@ 0403 KLM EPH

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Temp. ("C) Sample Date Asiatic LOCATION LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp. ("C) Clams/m3 Clam 11/03/2009 133 0 16 11/03/2009 76 16 In plant RCW CMW 11/10/2009 417 6.1 16 11/10/2009 25 16 lnplant RCW CMW 11/17/2009 269 0 16 11/17/2009 0 16 In plant RCW CMW 11/24/2009 36 50 15 11/24/2009 18 15 lnplant RCW CMW 12/01/2009 32 0 13.5 12/01/2009 0 13.5 In plant RCW WE 12/08/2009 38 0 11 12/08/2009 0 11 In plant RCW CMW 01/05/2010 0 0 6 01/05/2010 0 6 In plant RCW 8 01/12/2010 0 0 5 01/12/2010 0 5 In plant RCW 01/19/2010 0 0 6 01/19/2010 0 6 In plant RCW p 01/26/2010 32 0 7.5 01/26/2009 0 7.5 In plant RCW NRT 02/02/2010 0 0 7 02/02/2010 0 7 In plant RCW MSW/WDT 02/09/2010 0 0 8 02/09/2010 0 8 In plant RCW BLITC 02/16/2010 0 0 5 02/16/2010 0 5 In plant RCW BJ 02/23/2010 11.7 0 7 02/23/2010 0 7 In plant RCW BJ 03/02/2010 0 0 6 03/02/2010 0 6 In plant RCW PB 03/09/2010 0 0 8 03/09/2010 0 8 lnplant RCW MJW 03/16/2010 0 0 10 03/16/2010 0 10 In plant RCW BC 03/23/2010 14 0 11 03/23/2010 0 11 In plant RCW BC 03/30/2010 0 0 14 03/30/2010 0 14 lnplant RCW BAPO Apr-2010 no samples collected May-2010 no samples collected Jun-2010 no samples collected Jul-2010 no samples collected Aug-2010 no samples collected Sep-2010 no samples collected Oct-2010 no samples collected Nov-2010 no samples collected

December 6, 2010 Bradley M. Love, OPS 5N-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, NOVEMBER, 2010 Per your request, I am only submitting an electronic copy of the subject report. The report provides results of compliance testing using fathead minnows and daphnids. Outfall101, samples collected October 31-November 5, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services- West CEB2L-M Attachment cc (Attachment): Sherrard, R. M., PSC 1X-C Files, OE&R, MPB 1E-M SQN November 201 OM

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: December 6. 2010

1. Facility I Discharger: Sequoyah Nuclear Plant I TVA
2. County I State: Hamilton I Tennessee
3. NPDES Permit#: TN0026450
4. Type of Facility: Nuclear-Fueled Electric Generating Plant
5. Design Flow (MGD): 1.579
6. Receiving Stream: Tennessee River (TRM 483.6)
7. 1Q10: 3.491
8. Outfall Tested: 101
9. Dates Sampled: October 31-November 5, 2010
10. Average Flow on Days Sampled (MGD): 916.91. 918.12. 916.45
11. Pertinent Site Conditions: Towerbrom 960 (oxidizing biocide used for mollusk control) was injected into the RCW beginning 11/1110 @ 0900 through 1115110 @ 2359. During toxicity sampling three of the six condenser circulating water (CCW) pumps were in service and Unit 1 was shut down for a refueling outage.
12. Test Dates: November 2-9. 2010
13. Test Type: Short-term Chronic Definitive
14. Test Species: Fathead Minnows (Pimephalespromelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested(%): Outfall101: 11.3. 22.6. 45.2. 72.6. 100 Intake: 100.0 Pimephales promelas: UV treated Outfall101: 11.3. 22.6. 45.2. 72.6, 100 UV treated Intake: 100.0
16. Permit Limit Endpoint(%): Outfall101: IC2s = 45.2%
17. Test Results: Outfall101: Pimephales promelas: IC2s > 100%

Page 1 of105

Ceriodaphnia dubia: Kzs > 100% UV treated Outfall101: Pimephales promelas: ICzs > 100%

18. Facility

Contact:

Stephanie A. Howard Phone#: (423) 843-6700

19. Consulting I Testing Lab: Environmental Testing Solutions. Inc.
20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Cynthia L. Russell Phone #: (256) 386-2755

22. Notes: Outfall101 samples collected October 31-November 5, 2010, showed no toxic effects to fathead minnows or daphnids.

The resulting ICzs values, for both species, were> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page2of105

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101. Intake
2. Sample Type: Composite
3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. t TRC* Time (ET) ID Collected Received (OC) (mg/L) Last Used By 10-31-10 0743 to 11-02-10 1225 101 11-01-10 1456 0.8, 1.0 <0.10 11-01-10 0643 11-03-10 1125 10-31-10 0836 to 11-02-10 1225 Intake 11-01-10 1456 0.7 <0.10 11-01-10 0736 11-03-10 1125 11-02-10 0727 to 11-04-10 1240 101 11-03-10 1430 0.8, 0.8 <0.10 11-03-10 0627 11-05-10 1125 11-02-10 0738 to 11-04-10 1240 Intake 11-03-10 1430 1.0 <0.10 11-03-10 0638 11-05-10 1125 11-06-10 1130 11-04-10 0741 to 101 11-05-10 1500 1.2, 1.2 <0.10 11-07-10 1128 11-05-10 0641 11-08-10 1135 11-06-10 1130 11-04-10 0758 to Intake 11-05-10 1500 1.3 <0.10 11-07-10 1128 11-05-10 0658 11-08-10 1135

         *TRC = Total Residual Chlonne tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
4. Sample Manipulation: Samples from Outfall101 and intake were warmed to test temperature (25.0 + 1.0°C) in a warm water bath.

Aliquots of0utfall101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics. Inc.) for 2 minutes. Page3of105

Pirnephales prornelas Ceriodaphnia dubia Test Organisms:

1. Source: Aquatox, Inc. In-house Cultures
2. Age: 19.57- 19.75 hours old < 24-hours old Test Method Summary:
1. Test Conditions: Static, Renewal Static. Renewal
2. Test Duration: Until at least 60% of control females have 3 broods
3. Control I Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
4. Number of Replicates: .4 10
5. Organisms per Replicate: 10 1
6. Test Initiation: (Date/Time)

Outfall101 11-02-10 1145 ET 11-02-10 1225 ET UV Treated Outfall 101 11-02-10 1134 ET

7. Test Termination: (Date/Time)

Outfall101 11-09-10 1055 ET 11-09-10 1143 ET UV Treated Outfall 101 11-09-10 1040 ET

8. Test Temperature: Outfall101: Mean= 24.7°C Mean= 24.9°C (24.4 - 25.3°C) (24. 7 - 25.2°C)

Test Temperature: UV-Treated Outfall101: Mean = 24.8°C (24.4 - 25.2°C)

9. Physical I Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyyille, CA).

Page4 of lOS

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephales pro me/as Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted November 2-9. 2010 using effluent from Outfall101. Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 98 98 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 95 95 Mean Dry Weight (mg) Test Solutions (replicate number) (% Effluent) 1 2 3 4 Mean Control 0.767 0.766 0.956 0.837 0.832 11.3% 0.793 0.702 0.773 0.721 0.747 22.6% 0.698 0.737 0.800 0.803 0.760 45.2% 0.739 0.729 0.752 0.706 0.732 72.6% 0.725 0.802 0.773 0.790 0.773 100.0% 0.827 0.913 0.709 0.782 0.808 Intake 0.736 0.773 0.814 0.629 0.738 ICzs Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

               *TUa = 100/LCso: TUc = 100/ ICzs Page 5 of lOS

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted November 2-9. 2010 using effluent from Outfall101. Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 32 29 30 28 32 28 31 28 30 29.8 11.3% 32 32 30 32 28 32 30 33 29 30 30.8 22.6% 35 29 32 35 31 34 31 29 30 33 31.9 45.2% 36 34 31 34 36 36 32 30 34 33 33.6 72.6% 34 31 31 38 36 35 32 34 36 37 34.4 100.0% 39 35 36 36 34 37 34 38 33 36 35.8 ICzs Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

               *TUa = 100/LCso: TUc = 100/ ICzs Page 6 of lOS

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted November 2-9. 2010 using water from Intake Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 31 31 28 28 30 33 29 31 31 30.1 Intake 32 33 28 35 34 35 34 31 32 33 32.7 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

              *TUa = 100/LCso: TUc = 100IIC2s Page 7 of105

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted November 2-9. 2010 using effluent from UV Treated Outfall101. Test Percent Surviving Solutions (time interval used- days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg) Test Solutions reR_licate number) (% Effluent) 1 2 3 4 Mean Control 0.857 0.760 0.821 0.910 0.837 11.3% 0.879 0.908. 0.826 0.830 0.861 22.6% 0.828 0.868 0.951 0.731 0.845 45.2% 0.837 0.901 0.897 0.834 0.867 72.6% 0.820 0.829 0.854 0.952 0.864 100.0% 0.891 0.800 0.929 0.907 0.882 Intake 0.879 0.876 0.875 0.935 0.891 ICzs Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

              *TUa = 100/LCso: TUc = 100/ ICzs REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (ICzs) Pimephales promelas November 2-9, 2010 1155 7-days KCl 0.70 g/L Ceriodaphnia dubia November 2-9, 2010 1208 6-days NaCl 1.09 g/L Page8of105

PHYSICAUCHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pimephales promelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfull 101 perfonned November 02-09, 2010. Test Sample ID 0 Temperature ( C} Dissolved Oxygen (mg/L) pH(S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (!lmhos/cm) (mg!L CaC0 3) (mg!L CaC03 ) Chlorine (mg!L) 24.8 24.6 7.6 7.2 7.75 7.64 315 63 91 Control 24.7 - 24.9 24.5 - 24.7 7.5 - 7.7 6.6 - 7.6 7.67 - 7.79 7.43 - 7.82 305 - 333 62 - 64 89 - 93 24.8 24.6 7.7 7.2 7.84 7.62 300 11.3% 24.7 - 25.0 24.4 - 24.9 7.5 - 7.9 6.6 - 7.6 7.79 - 7.92 7.39 - 7.82 290 - 310

              .s                          24.9          24.7            7.7         7.1          7.83          7.60        286
                ~          22.6%

24.7 - 25.0 24.6 - 24.8 7.5 - 8.0 6.4 - 7.7 7.80 - 7.92 7.37 - 7.80 280 - 295 l:!:)., 24.9 24.7 7.8 7.1 7.82 7.60 257 tl 45.2%

              -a                      24.7 - 25.0   24.5 - 24.8    7.5 -    8.1 6.4 -    7.6 7.80 - 7.90   7.37 - 7.78  250 - 266
              't           72.6%

24.9 24.6 7.8 7.1 7.80 7.59 220

              .§ Q,

24.7 - 25.1 24.5 - 24.8 7.5 - 8.0 6.5 - 7.7 7.76 - 7.89 7.35 - 7.78 204 - 229 24.9 24.6 7.8 7.1 7.79 7.59 186 69 71 <0.10 100.0% 24.7 - 25.3 24.5 - 24.8 7.5 - 8.1 6.5 - 7.7 7.72 - 7.89 7.35 - 7.78 179 - 192 67 - 71 70 - 72 < 0.10 - < 0.10 24.9 24.6 7.9 7.2 7.76 7.59 187 67 69 < 0.10 Intake 24.8 - 25.2 24.4 - 24.8 7.6 - 8.2 6.7 - 7.8 7.68 - 7.89 7.36 - 7.76 182 - 191 67 - 67 68 - 70 < 0.10 - < 0.10 24.8 24.9 7.6 7.7 7.75 7.82 315 63 91 Control 24.7 - 24.9 24.7 - 25.2 7.5 - 7.7 7.4 - 8.0 7.67 - 7.79 7.74 - 7.90 305 - 333 62 - 64 89 - 93 24.8 24.9 7.7 7.8 7.84 7.83 300 11.3% 24.7 - 25.0 24.8 - 25.2 7.5 - 7.9 7.5 - 8.0 7.79 - 7.92 7.71 - 7.90 290 - 310

              .s
              '"'l                        24.8          25.0            7.7         7.8          7.83          7.82        286 22.6%
              ~                       24.7 - 25.0   24.8 - 25.2    7.5 -    8.0 7.5 -   8.0  7.80 - 7.92   7.69 - 7.90  280 - 295
              .s                          24.9          25.0            7.8         7.7          7.82          7.81        257
              ..:::==      45.2%
                §-                    24.7 - 25.0   24.8 - 25.1    7.5 -    8.1 7.5 -   8.0  7.80 - 7.90   7.68 - 7.88  250 - 266
              '1:1                        24.9          25.0            7.8         7.7          7.80          7.81        220
              *~           72.6%

24.8 - 25.0 24.7 - 25.2 7.5 - 8.0 7.4 - 8.0 7.76 - 7.89 7.68 - 7.87 204 - 229 t:l 25.0 24.9 7.8 7.7 7.79 7.80 186 69 71 < 0.10 100.0% 24.8 - 25.2 24.7 - 25.2 7.5 - 8.1 7.5 - 8.0 7.72 - 7.89 7.67 - 7.86 179 - 192 67 - 71 70 - 72 < 0.10 - < 0.10 24.9 25.0 7.9 7.8 7.76 7.81 187 67 69 < 0.10 Intake 24.7 - 25.1 24.9 - 25.2 7.6 - 8.2 7.5 - 8.0 7.68 - 7.89 7.70 - 7.88 182 - 191 67 - 67 68 - 70 < 0.10 - < 0.10 Overall temperature ("C) Average Minimum Maximum Pimephales promelas 24.7 24.4 25.3 Ceriodaphnia dubia 24.9 24.7 25.2 Page 9ofl05

PHYSICAUCHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges fur Pimephales promelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfull 101 perfunred November 02-09. 2010. Test Sample ID Temperature ('C) Dissolved Oxygen (mg!L) pH(S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (ltmhos/cm) (mg!L CaC03 ) (mg!L CaC03 ) Chlorine (mg!L) 24.9 24.7 7.8 7.2 7.82 7.62 313 60 84 Control 24.7 - 24.9 24.6 - 24.8 7.5 - 8.0 6.6 - 7.7 7.77 - 7.88 7.28 - 7.82 301 - 329 60 - 61 82 - 86 24.9 24.7 7.8 7.2 7.82 7.61 296 11.3%

                 ..,                 24.9 - 25.0  24.5 - 24.8   7.5 -    8.0  6.6 -    7.8 7.78 - 7.88   7.31 - 7.80   290 - 303
               .s~

25.0 24.7 7.8 7.2 7.82 7.61 282 IS 22.6% 24.9 - 25.0 24.5 - 24.8 7.5 - 8.1 6.6 - 7.7 7.78 - 7.88 7.32 - 7.78 277 - 290

                 ~
                 ~                       25.0         24.6           7.8          7.1          7.81          7.59         256 Jl':1       45.2%

24.9 - 25.0 24.4 - 24.8 7.5 - 8.1 6.5 - 7.7 7.77 - 7.87 7.31 - 7.78 250 - 260

                 ~                       25.0         24.6           7.8           7.1         7.80          7.60         223 72.6%
               .§                    24.9 - 25.1  24.5 - 24.7   7.5 -    8.1  6.6 -    7.7 7.76 - 7.86   7.32 - 7.78   217 - 228
               =...

25.0 24.6 7.9 7.1 7.79 7.59 189 68 72 < 0.10 100.0% 24.9 - 25.2 24.4 - 24.9 7.5 - 8.1 6.5 - 7.7 7.73 - 7.87 7.29 - 7.78 184 - 196 67 - 69 70 - 74 < 0.10 - < 0.10 25.0 24.6 8.0 7.2 7.76 7.58 186 68 73 < 0.10 Intake 24.9 - 25.1 24.4 - 24.8 7.6 - 8.3 6.6 - 8.0 7.68 - 7.84 7.30 - 7.80 178 - 193 64 - 71 70 - 78 < 0.10 - < 0.10 Overall temperature ('C) Average Minimum Maximum Pimephales promelas 24.8 24.4 25.2 Page 10 of 105

SUMMARY

I CONCLUSIONS Outfall101 samples collected October 31-November 5, 2010, showed no toxic effects to fathead minnows or daphnids. The resulting ICzs values, for both species, were> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 11 of I 05

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None
2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
1. Pimephales promelas None
2. Ceriodaphnia dubia None Page 12 of 105

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
3. Temperature was measured by SM 2550 B.
4. Dissolved oxygen was measured by SM 4500 0 G.
5. The pH was measured by SM 4500 H+ B.
6. Conductance was measured by SM 2510 B.
7. Alkalinity was measured by SM 2320 B.
8. Total hardness was measured by SM 2340 C.
9. Total residual chlorine was measured by ORION Electrode Method 97-70.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as ICzs values in giL KCl or NaCl.
2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.
4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 13 of 105

REFERENCES

1. NPDES Permit No. TN0026450.
2. USEP A. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
3. Standard Methods for the Examination of Water and Wastewater. 21st Edition, 2005.
4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 14 ofl05

Sequoyah Nuclear Plant Biomonitoring November 2-9, 2010 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of105

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- November 5, 2010 Date PCL-401 mg!L Copolymer 0.005 0.011 0.021 0.019 O.DlS O.DlS O.DlS 08/18/1999 0.024 08/19/1999 0.024 08/20/1999 0.024 08/21/1999 0.024 08/22/1999 0.024 08/23/1999 0.024 08/24/1999 ' 0.023 Page 16 of 105

Table B-1. Sequoyah Nuclear Plant Diffuser (OutfalllOl) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- November 5, 2010 Date PCL-401 mg/L Copolymer 0.009 0.028 0.009 0.009 0.009 0.009 0.009 0.019 0.019 0.018 0.019 0.019 0.019 0.019 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.021 0.021 0.021 0.020 0.021 0.020 11/26/2001 0.02 11/27/2001 . 0.019 11/28/2001 0.019 11/29/2001 0.02 11/30/2001 0.02 12/09/2001 12/10/2001 12/11/2001 12/12/2001 0.02 12/13/2001 0.02 12/14/2001 0.02 Page 17 ofl05

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- November 5, 2010 Date 10/07/2002 10/08/2002 ' 10/09/2002 10/10/2002 10/11/2002 01/12/2003 01/13/2003 01/14/2003 01/15/2003 01/16/2003 01/17/2003 003 04/07/2003 . 04/08/2003 04/09/2003 04/10/2003 04/11/2003 Page 18 of105

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998- November 5, 2010 Date PCL-401 mg/L Copolymer 0.020 0.014 0.014 0.020 0.020 08/04/2003 0.020 08/05/2003 0.020 08/06/2003 0.020 08/07/2003 0.020 08/08/2003 0.020 10/05/2003 0.020 10/06/2003 0.020 10/07/2003 0.020 10/08/2003 0.020 10/09/2003 0.022 10/10/2003 0.024 0.009 02/02/2004 0.009 02/03/2004 0.009 02/04/2004 0.009 02/05/2004 l.?f.\\'W\'\0;\'q,z,'. 0.009 02/06/2004 0.009 05/04/2004 0.019 05/05/2004 0.014 05/06/2004 0.013 05/07/2004 0.020 05/08/2004 0.021 05/09/2004 0.020 07/04/2004 0.019 07/05/2004 0.020 07/06/2004 0.020 07/07/2004 0.019 07/08/2004 0.019 07/09/2004 0.020 Page 19 of 105

Table B-1. Sequoyah Nuclear Plant Diffuser (OutfalllOl) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- November 5, 2010 Date 11/07/2004 11108/2004 11109/2004 11/10/2004 11/11/2004 11/12/2004 02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/1112005 06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10/2005 07/17/2005 07/18/2005 07/19/2005 07/20/2005 07/2112005 07/22/2005 10/30/2005 100:':'""'""?=1 10/3112005 11/0112005 11102/2005 11103/2005 11104/2005 11114/2005 0.0274 11/15/2005 0.0256 11116/2005 0.0234 11117/2005 0.0231 11118/2005 0.0200 11119/2005 0.0116 Page 20 of 105

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998- November 5, 2010 Date Cuprostat-PF mg/L Azole O.Q15 O.Q15 0.015 O.Q15 O.Q15 O.Q15 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/13/08 04/14/08 04/15/08 04/16/08 04/17/08 04/18/08 0.030 0.030 0.030 Page 21 of 105

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- November 5, 2010 Page 22 of 105

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- November 5, 2010 Date MSW 101 mg/L Phosphate 10/31/10 11/01/10 11/02/10 11/03/10 11/04/10 11105/10 Page 23 of105

Sequoyah Nuclear Plant Biomonitoring November 2-9, 2010 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 24 of 105

BIOMONITORING CHAIN OF CUSTODY RECORD Page __l __ of __ l __ Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specifY): Express Courier 28801 uc;;uc::.nu Comments: Facility Sampled: Sequoyah NP AdamDeimling ~~ NPDES Number: TN0026450 Phone: 828-350-9364 Tony A. Knight ~ * ~=--'-1-:-j~~~~!l...._---- Fax: 828-350-9368 Samples remained on t:VroughoW sampling and transport to Collected By: Adam Deimling & Tony A. Knight lab. Dissolved Metals filtered and place on ice. Field Identification I 1 Grab/Comp Collection Datelfime Container Flow Sample Description Number& (MGD) Rain Event? Volume (Mark as Appropriate) No I Trace X SQN-101-TOX B Comp 1 (2.5gal) I 916.91 X

                                                          'lt IV           VV"TJ 10/31110-            0836-SQN-INT-TOX                Comp 0736 I (2.5 gal)  I   NA                             X 11/1/10 Relinquished By (Signature):                                Date/Time                               Received By (Signature):

Adam Deimling ;t/J/M tots- f:f)1 Jl Jo Express Courier I '-tSI.. !.'\" IETS Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

I*~~~~~~~~,,_ .. _, ~~"-~- ~",, ~*~*" ,_, ,__._,_-.._...: -"-"--'-""""-~~~~-..:."'"*-~=-~=*==--"""""--'--=-- .,._ *~*z=*-*--~ .h--~....,_-~ BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of_l_ Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 Comments: ./~ ~ Adam Deimling : ~ c:;..:; ~ NPDES Number: TN0026450 Phone: 828-350-9364 Jijea Uitebel! * ~.L----------- Fax: 828-350-9368 Samples remained on ice throughout sampling and transport to Collected By: Adam Deimling & Ben Mitchell lab. Dissolved Metals sample filtered and place on ice. Field Identification I 1 Grab/Comp Collection Date!fime Flow Sample Description (MGD) Rain Event? (Mark as Appropriate) No I Trace 0727-SQN-101-TOX A I Comp l11t21l0-11131lo 1 0627 I 1(2.5gal) I 918.12 X 0727-SQN-101-TOXB I Comp 1 1112110- t1131lo 1 0627 I I (2.5gal) I 918.12 X 0738-SQN-INT-TOX I Comp 1 1112110- 1113/to 1 0638 I I (2.5 gal) I NA X Relinquished By (Signature): Dateffime Received By (Signature): Adam Deimling flp/tc:; 1l-o~ *Jo ET Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page __ l __ of __l __ Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 3 51 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 GeneralComments: ~ Adam Deimling  :.___{._.L..._--=...~---,.--=

                                                                                                                                                                                      ~-----.-9z-------

NPDES Number: TN0026450 Phone: 828-350-9364 Jonathan Walker  :.___.;ZE:.!312!::...=:--==-.~~~== e' Fax: 828-350-9368 Samples remained on ice throughout sampling and transport to Collected By: Adam Deimling & Jonathan Walker lab. Dissolved Metals sample filtered and place on ice. Field Identification I Sample Description I Grab/~omp I Collection Date/fime I Container Number& I Flow (MGD) I Rain Event? (Mark as Appropriate) Yes IfYes, I No Inches I VI""T.& SQN-101-TOX A I Comp 1114/10- 1115110 0641 1 (2.5gal) 0.01 I X 0741-SQN-101-TOX B Comp 11/4/10- 11/5110 I (2.5gal) I 916.45 I I 0.01 I I X 0641 0758-SQN-INT-TOX Comp 11/4110- 1115110 I 1 (2.5 gal) I NA I I 0.01 I I X 0658 Adam Deimling Er Express Courier ll*OS-tO tSOO ET Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Ir 'I Page 1 of6

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          ..) Environmental Testing Solutions, Inc.

I Chronic Whole Effluent Toxicity Test (EPA--821-R-02-013 Method 1000.0) Client: Tennessee Valley Authority Facility: Seguoyah Nuclear Plant NPDES #: TN0020168 Species: Pimeohales oromelas County: Rhea Outfall: 101 Project#: -.:::'-....~.1..wo~~L------ n Dilution preparation i"{ormation: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effiuent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0 Total volume (mL) 2500 2500 2500 2SOO 2500 Test orxanism information: Test information: Organism age: ~~~ tlA.tAC. OLb Randomizing template: 'le.\.\.OI.U Date and times organisms u*0\*1 o llo~O Incubator number and were born between: shelf location: .3C... Organism source: J1.T())C. S~t\ Pf l\-o\- 10 Artemia CHM number: C.Ht1Sil Drying information for weight determination: Transfer bowl information: pH= "'\.1"\ s.u. Date I Time in oven: 1\-oat-10 tnS Temperature =1$-~C Initial oven temperature: r.. b*C. Average transfer volume: Date I Time out of oven: 1\-10*10 IUS

0. \ '!1'-H......Q Final oven temperature: ~~*c.

Total drying time: bl-&k\dc$ Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW

                                                                                           . renewal, or                                                 batch I                                                                                                        SOP AT20 - Exhibit AT20.3, revision 04-01-09 lage 18 of105

I, .=Er*s Page 2 of6 I l.

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Envlranmontoi'IHtlng Solutions, Inc. Il I

 ~*
     .                         Species: Pimeohales prome/as Client: TVA I Seguoyah Nuclear Plant, OutfalllOl. Non-treated                     Date:    -.::..:;1\....;:*01.=-:*1..:0_ _
   .                                                                              Survival and Growth Data Day                                                 U.Jo/o                            22.6 11/o I                                               0 A     B    C   D      E        F      G      H       I           J                K       L ID IO       IO  10     lb       IO 10 10             IO         I{)              10        /0 m                                               1 tO     10        It>   10     IO      10           IO /0                  /()

IO ID lb I 3 2 IO 10 lC /{) /Q *flj 10 IC /Q IO 10 10 lD

                                                              /tJ   It;) I~   It)   I~       /C      lO     IO     /0       10                10 I                                              4 10     IC   IO   10   I~      IQ       IO     10    IC        IO                /Q        10 s

i 10 It) (() IQ IO 10 fl) to /() 10 IO IO -i 6 7

                                                               /Q IO l(J IO 10 IO   ,~ IO ID 10 IQ 10 IO IO IO I{)

I() I() IC IO 10 IO

                                                                                                                                                         /Q IO I                             B = Pan + Larvae weight (mg)

Analyst: M.tk Date: ll It:\

  • lO C =Larvae weight (mg)
                              *B-A Weight per initial number of larvae (mg)
                              = C I Initial number of larvae Average              Percent weight per           reduction initial              from       o. 1~2.                o.....,          10.1'1.       o.1r.c number of            control(%)

Iarvae(mld Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.

                                                                                                                                                            -tJt-I Calculations and data reviewed:
}

I i CommenJs: SOP AT20- Exhibit AT20.3, revision 04-01-09

  *Page 29 of 105

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           ,) Environmental Testing Solutions, Inc.

Page 3 of6 I Species: Pimeohales oromelas Client: TVA I Seouoyah Nuclear Plant, Outfall tOt. Non-treated Date: 1\-at-10 ~~ Survival and Growth Data Day 45.2% 72.6% 100% M N 0 p Q R s T u v w X I 0

                                                                  /0       /0      /Q      IO        IC        10      IO      IO      IO       'O          10       /Q I

l IC lb to 10 I(> It> IO {l) 10 10 f() ID

*:'*                                         2
                                                                  /Q      /D      I~       IO        10        /0      /0      lb       IO       ID 10              IO J;

3 4 s IO

                                                                  /D tO 10 IC 10 IO
                                                                                           /Q IC IQ IQ to lQ
                                                                                                                       /()

IQ

                                                                                                                               /(j IO IC IC lO 10 10 10
                                                                                                                                                                   /Q 10      /()    to      ll>       LO          l()     10 lO            /()       10       /C      10 6
                                                                /\)       1\)    IO       1\'tl\ fD             10 IO          lO       ID IC              10        to 7                                                                                                     ltll"
                                                                 /D       10     10        Ct. I()         I0      /0     /0       /0        10        10       /Q A= Pan weight(~

Tray coloO'ide: Ji~ Analyst: \'\.~~ \'>A'i> \~ \'l*qyt \~~ \~ilt (')~~ ~ \\~ fj.\\) \~;ft \,~\ . Date: ~\L O'J,o. \0 B = Pan + Larvae weight (mg) Analyst: M.\kt t,f...O\ f.,f.01 u. ..cn ro"~ -z,t,. 0 I f,t..11\ t.,t.1l !0-"'f ~~.~ t4.'L~ t-l. B(.. 'Ztl.'t"\ Date: \\ * \.0 .to C =Larvae weight (mg)

                    =8-A 1.1~    1.4io                       ,,oc; 1.tz..
                                                                                                                                               "*'~

1.t.1 1.1'\ ,.~'Z. "J,OI. 1.1..S c!.G1. t.11 Weight per initial number

                                                                                                   ,..."'~              ~ ()*"o.,O \,~
                                                                                  ""'~ o*,...~                 '4:.~                                                 ~+.,\,.

of larvae (mg)

                                                                "'bo...  ~0..

o* o* fA.~ ~

                    = C I Initial number of larvae

()* ~ O* ()* o* C>* 0* o* Average Percent weight per reduction initial from control 0.1!>1-- ,2..()7. 0.11~ 1.\7. o.&ot 't.l!e1* number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: _i._ Comments: i Page 30 of 105 SOP AT20 -Exhibit AT20.3, revision 04-0 l-09

I*:;-;,' i=ETS

   ~
              ,) Envtronmontalllostlng Solutions. Inc.

Page4 of6 'I. II Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant. OutfalllOl. Non-treated Date: _,.!,1\.L.*~n~*u.IO,__

-I                                      Day 0

1

                                                              /0   10 IC>

I 2 3

                                                          /0  10   10 ID   /0  /0 I                                       4 IC /C   lD 5

I 6 lfJ ID LD IC I D lO I 7

                                                          /0    /0 /0 I

I I per I of larvae (mg)

                     = C I Initial number of larvae Average                Percent I                  weight per initial number of reduction from control

(%) I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk =sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.

                                                                                                                                             -di-I Calculations and data reviewed:

I I Comments: I I Page 31 of 105 SOP AT20 - Exhibit AT20.3, revision 04-01-09

~ ~ ~ TVA I Sequoyah Nuclear Plant, OutfalllOl Non-treated ~ ...... November 02-09, 2010 ~ v-,

        **                                                                                               Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control
      @                                                                                             Verification of Data Entry, Calculations, and Statistical Analyses
      ~          Environmental Testing Solutions, Inc.                                                                                                                                                                                    Project aumber:

ft~13Wt:U oy; ,-- ""~~ ~ ... 6709 Not for Compliance Assessme~tl, Internal Laboratory QC .l c_......-l%l ......... t.Hial*a..a.rf:l l"lDDII**--oliHYae A*Pao ......t('"l) B*Putl.arvae

                                                                                                  ......t('"l)

Lanao ....... ('"l)

                                                                                                                     -A*B Welpt I Sanh1q
                                                                                                                                    ....... r~~~uv.. <-.> Slll'\'h1.qa...t.rot Coeflideat fllvartaao.

(M-.wtpc ........... WefPt/laitialaa.t.er

                                                                                                                                                                                                         ......... ('"1)

Me&~~nii'Vival (%) ***beroi.Jano.,

                                                                                                                                                                                                                                                                 ~.,
                                                                                                                                                                                                                                                            .......... <Me- ......
                                                                                                                                                                                                                                                                                       -   -(.........
                                                                                                                                                                                                                                                                                               % ) ,... !

lonoe('"l) ......... 11.-9.,(%) (*I) .......................... 1%1 A 10 10 12.85 20.52 7.67 0.767 0.767 B 10 10 13.20 20.86 7.66 0.766 0.832 10.8 0.766 100.0 0.832 10.8 Coatrol Not *ppllarble c 10 10 13.77 23.33 9.56 0.956 0.956 D 10 10 13.25 21.62 8.37 0.837 0.837 E 10 10 13.96 21.89 7.93 0.793 0.793 F 10 10 14.15 21.17 7.02 0.702 0.702 11.3% 0.747 5.7 100.0 0.747 5.7 10.1 G 10 10 14.74 22.47 7.73 0.773 0.773 H 10 10 12.53 19.74 7.21 0.721 0.721 I 10 10 14.33 21.31 6.98 0.698 0.698 J 10 10 13.02 20.39 7.37 0.737 0.760 6.7 0.737 ll.6% 100.0 0.760 6.7 8.7 K 10 10 12.60 20.60 8.00 O.SOO o.soo L \0 \0 13.48 21.51 8.03 0.803 0.803 M 10 10 14.62 22.01 7.39 0.739 0.739 N 10 10 13.78 21.07 7.29 0.729 0.729 45.2% 0.751 3.2 97.5 0.732 l.7 12.0 0 10 10 15.01 22.53 7.52 0.752 0.752 p 10 9 13.87 20.93 7.06 0.784 0.706 Q 10 10 14.76 22.01 1.25 0.725 0.725 R )0 10 14.77 22.79 8.02 0.802 0.802 72.6% 0.773 4.4 100.0 0.773 4.4 7.1 s 10 10 13.98 21.71 7.73 0.773 0.773 T 10 10 13.04 20.94 7.90 0.790 0.790 u 10 10 14.78 23.05 8.27 0.827 0.827 100% v 10 10 15.10 24.23 9.13 0.913 0.808 10.6 0.913 100.0 0.808 10.6 2.9 w 10 10 14.77 21.86 7.09 0.709 0.709 X 10 10 13.91 21.73 7.82 0.782 0.782 y 10 10 13,89 21.25 7.36 0.736 0.736 100%la18ke z 10 10 12.82 20.55 7.73 0.773 0.777 4.2 0.773 95.0 0.738 10.8 11.2 AA 10 10 14.73 22.87 8.14 0.814 0.814 BB 10 '--- 8 13.87 20.16 6.29 0.786 0.629 --- - Outfa!l101: MSD- Minimum Significant Difference Duaaett's MSD v_.ae: 0.1014 PMSD~ Percent Minimum Significant Difference PMSD: 11.2 PMSD is a measure of test precision. The PMSD is tho minimum percent diff"""""' between the control and treatment that can be declared statistically significant in a whole oftluenttoxicity test.

      !!!!l!!ti O.aaett's MSD value:            0.1161                                                   Lower PMSD bound detennined by USEPA (lOth percentile) - 12"!..

PMSD: 14.0 Upper PMSD bowul detennined by USEPA (90th percentile)~ 30".4. Lower and upper PMSD bounds wen: determined from the I Otb and 90th percentile. respectively, of PMSD data from EPA's WET lntedaboratoty Variability Study (USEPA. 2001a; USEP A. 200 I b). US EPA. 200Ia, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effiuont Toxicity Tesl Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

~ ~ TVA I Sequoyah Nuclear Plant, OutfalllOl ~

    .ETS Non-treated

~ ~ *** November 02-09, 2010 Statistical Analyses c ':'*.,i*>'? " **<***: ',_.***:""'

    ~      Environmental Testing Solutions, Inc.

Larval Fish Growth and Survival Test-7 Day Growth Start Date: 111212010 TestiD: PpFRCR SampleiD: TVA I Sequoyah Nuclear Plant, OutfalllOl End Date: 11/912010 LabiD: ETS-Envir. Testing Sol Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Cone-% 1 2 3 4 D-Control 0.7670 0.7660 0.9560 0.8370 11.3 0.7930 0.7020 0.7730 0.7210 22.6 0.6980 0.7370 0.8000 0.8030 45.2 0.7390 0.7290 0.7520 0.7060 72.6 0.7250 0.8020 0.7730 0.7900 100 0.8270 0.9130 0.7090 0.7820 Intake 0.7360 0.7730 0.8140 0.6290 Transform: Untransformed  !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.8315 1.0000 0.8315 0.7660 0.9560 10.753 4 0.8315 1.0000 11.3 0.7473 0.8987 0.7473 0.7020 0.7930 5.726 4 2.002 2.410 0.1014 0.7637 0.9185 22.6 0.7595 0.9134 0.7595 0.6980 0.8030 6.723 4 1.711 2.410 0.1014 0.7637 0.9185 45.2 0.7315 0.8797 0.7315 0.7060 0.7520 2.657 4 2.377 2.410 0.1014 0.7637 0.9185 72.6 0.7725 0.9290 0.7725 0.7250 0.8020 4.379 4 1.402 2.410 0.1014 0.7637 0.9185 100 0.8078 0.9714 0.8078 0.7090 0.9130 10.569 4 0.565 2.410 0.1014 0.7637 0.9185 Intake 0.7380 0.8876 0.7380 0.6290 0.8140 10.751 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96612942 0.884 0.454275258 0.47255445 Bartlett's Test indicates equal variances (p = 0.19) 7.42590094 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.10139478 0.12194201 0.0057389 0.00354019 0.205096915 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point  % SD 95%CL(Exp) Skew IC05* 6.9292 ICIO >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100

      "' indicates IC estimate less than the lowest concentration                                                                                                                         sqnJOJ_J 10210data

~ ~ TVA I Sequoyah Nuclear Plant, OutfalllOl- Intake ~ ~ Non-treated ~ ..... November 02-09, 2010

       .ETS

~ 1.;,

     ***
  • Statistical Analyses 0---~- _ ,,,..,-,._,-.
                  . l'ffl{'       :*:-*-*
                                  "'**     -.-::*j;**---
     , ) Environmental Testing Solutions, Inc.

Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11/2/2010 TestiD: PpFRCR SampleiD: TVA I Sequoyah Nuclear Plant, Intake End Date: 1119/2010 LabiD: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Cone-% I 2 3 4 D-Control 0.7670 0.7660 0.9560 0.8370 11.3 0.7930 0.7020 0.7730 0.7210 22.6 0.6980 0.7370 0.8000 0.8030 45.2 0.7390 0.7290 0.7520 0.7060 72.6 0.7250 0.8020 0.7730 0.7900 100 0.8270 0.9130 0.7090 0.7820 Intake 0.7360 0.7730 0.8140 0.6290 Transform: Untransformed 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.8315 1.0000 0.8315 0.7660 0.9560 10.753 4 11.3 0.7473 0.8987 0.7473 0.7020 0.7930 5.726 4 22.6 0.7595 0.9134 0.7595 0.6980 0.8030 6.723 4 45.2 0.7315 0.8797 0.7315 0.7060 0.7520 2.657 4 72.6 0.7725 0.9290 0.7725 0.7250 0.8020 4.379 4 100 0.8078 0.9714 0.8078 0.7090 0.9130 10.569 4 Intake 0.7380 0.8876 0.7380 0.6290 0.8140 10.751 4 1.564 1.943 0.1161 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96937436 0.749 0.2255294 -0.7676666 F-Test indicates equal variances (p = 0.85) 1.26977658 47.4672279 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.11614059 0.13967599 0.0174845 0.0071445 0.16876405 1, 6 Treatments vs D-Control sqnl01_110210data

~                                                       TVA I Sequoyah Nuclear Plant, Outfall lOt - Non-treated

~

~

November 02-09,2010 ..a.....

~
        .ETS e , .;*.,. . ,,. . . ~
        ~
                               " '"* * . :~**.* ..,:.-,

Environmental Testing Solutions, Inc. Pimephales promelas Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses 6709 Control ll.lo/o

Z:Z.6o/o 4S.:Zo/o 72.6%

IOOo/o

I r *Ers

~ ,.,., ... . ,*., .~ . -~~~,

Page 5 of6 l'!,""

             ~  Environmental Testing Solutions, Inc.

I Species: Pimeohales promelas Client: TVA I Seguovah Nuclear Plant. OutfalllOl. Non-treated I I I I' CONTROL Non-treated I I 11.3% i Z2.6%

~
  • ~ 45.2%

72.6%

~
~
~

100%

~
~

I too*1o Intake I I Page 36 of 105 SOP A1'20- Exhibit A1'20.3, revision 04-0 l-09

Page 6 of6 Concen-tration CONTROL 1-ll!~~L---J-~~- Non-treated ll.Jo/o 22.6o/o 45.2o/o 72.6o/o lOOo/o lOOo/o Intake SOP AT20- Exhibit AT20.3, revision 04-01-09 Page 37 of 105

Page I of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species:' Ceriodaohnia dubia Client: Tennessee Valley Authority County: Hamilton Facility: Seguoyah Nuclear Plant Outfall: 101 NPDES #: TN0026450 Project #: _..;'AIIOI~'-~....ow'\L...-_ _ __ Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effiucnt volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685' 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism source in,(ormation: Test information: Organism age: < 24-hours old Randomizing template color: ~\,.\)f. Date and times organisms were born t\*01.*10 01ot T'O 17.00 Incubator number and shelf between: 1. ~,.. location: Culture board: IU*1,b *10 Replicate number: 1_12_13 4j_Sj_6J7IBI9 10 YWfbatch: Lll)'"()(*lO Culture board cup number: 1.111-S 1'1.& "tt\ Itt\ 11>>1.1 ~I !I\ 1* li1 Transfer vessel information: I pH= 1*1\ s.u. Temperature = "Z.'\

  • 111 "C Averagetransfervolume(mL): I ~*01.1.\~

Selenastrum batch: tl"'()l*IO Daily renewal information: Day Date MHSW Analyst batch used 0 2 3 4 5 6 7 Control information: Summary of test endpoints: Control-I Control-2 Acceptance criteria

 %of Male Adults:                                  07.               07..                     s 2()0/c,            7-day LCso           ') 10()1 ..
 %Adults having 3ra Broods:                         /007.            coo1.                     <:80%               NOEC                  1007.
 % Mortality:                                         07.              n7.                     s 200/c,            LOEC                 >1001.

Mean Offsprinz/Female: "2:'\.& --~*' ChV 2: 15.0 offspring/female >JOOT,

 %CV:                                             ~. "Z.7.           _5! 17.                 <40.0%                IC25                  >1001.

SOP AT II - Exhibit AT II.2, revision 04-0 1-09 38 of lOS

Page 2 of7 Species: Ceriodaohnia dub/a Client: TVA I Seguoyah Nuclear Plant. Outfall lOt Date: ---:.;l\,_*=(SJ.;...;...;*I-=0'----- CONTROL-1 Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced (") D D c 0 D 0 0 0 0 Adult mortality. L- L- \.._ '-- \.._ L \_ '-- \... '-- l Young produeed 0 ('") Q 0 () D c 0 C) (j Adult mortality L '-- L. L L

                                                                                      \.....             '-      '--                         '--                                    '--

3 Young produeed ('. 0 c...; 0 D c 0 0 0 {') Adult mortality

                                                          '- '-- '--                               \.._
                                                                                                               '-           '-- '--                       \....         '---       \.._

4 Young produeed '-\ '-t '-\ ~* ~ "\ '-\ s "\ '-\ Adult mortality

                                                         '-              \....       '-              '--        '-           '-- '-                       L            '-- ' -

5 Young produced n ...

                                                                                                                *~
                                                                                                   \~                                                                              \\
                                                                     *~\....

1\ I() '\ l\ l() Adult mortality '- '- '- '-- \.... \.....

                                                                                    '--                                                                 '-               \....

6 Young produeed 0 0 b ~ b D 0 0 a (') Adult mortality "-- '-- '-- \..._:.

                                                                                                               "-           '--           '--           L..           '--         '--
                                                                      ,c::;.
                                                                                                                                                                      *~

7 Young produced 1'5 \"S *4 \\o L.Q \C:, \'S Total young produeed

                                                       ,aao           ~'2.         1.'\          '"'
                                                                                                 .!to         'll             '!11.         '28         ...3\

l<f 30 c::.. ........... Final Adult Mortality \..... '- \.... \..... '-- '-- ~ X for 3ra Broods Note. Adult mortality (L

  • bve, D
  • dead),
                                                          }<..       _;><...        ><-

SB

  • split brood (smgle brood split between
                                                                                                   ~           "'><..        'fl'-            "><-        ><-          "::><..

two clays), CO

  • oany over (oftipring oamed over With edult during transfer).
                                                                                                                                                                                    )<..

Concentration:

                                                                                                                        % Mortality:                                       I 01 Mean Offspring/Female:                             I "2.q,.t CONC:       11.3%                                                                    Survival and Reproduction Data Reolleate number Day                                             1              l             3             4            5            6              7            8               9          10 1         Young produeed                     0               0           0            0            0            0                0           0             6           0 Adult mortality                    \.....       L-          \.....           \.._           \.._          L             L..           L             \.._      L.

l Young produeed c 0 0 0 0 0 0 a 0 . 0' Adult mortality '-- L._ L. L.. '- \..... '-- '-- \..... \...... 3 Young produced 0 b ('\ (") 0 CJ 0 0 0 0 Adult mortality L L.. \...... L L L. L L L '-- 4 Young produeed \4. ~ ""\ '-\ ~ '-\ ~ ~ '-\ Adult mortality \.... '- "" L. \..... \...... '- L. L... \.._ '- 5 Young produced l~ \C to 11. 1\ I '"'2- \ \ l'b a{) 10 Adult mortality '-- \._ L '- '-- '-- L. \.._ L 6 Young produeed 0 () (") () 0 () 0 0 0 a Adult mortality '-- '-- '-- \.._ '-- L.. '--

                                                                                                \.......      '--         '--
                                                                                                                                             ~~
                                                        '~

7 Young produced \\ ~~ tc&

                                                                                                                                                                                    *~

llD 11 1'-( Total young produced 31.. 31. ao 32.. 2~ .31. ~ ~3> 2.'i 30 Final Adult Mortality -'- L. '- '- '-- -'- "- '-- '-- '-- Note: Adult mortality (L = live, D

  • deed), SB
  • split brood (single brood split between two days), CO
  • oa over ofl!lorina carried over with adult durina transfer).

Concentration:

                                                                                                                       % Mortality:                                             01.

Mean Offspring/Female: ao.t

                                                                                                                       % Reduction from Control- I:                           -3.'tl~

SOP ATll - Exhibit ATll.2, revision 04-01-09 39of105

Page 3 of7 Species: Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant. OutfalllOl Date: I Hit*IO coNe: 22.6% Survival and Reproduction Data Replicate number Day 1 l 3 4 5 6 7 8 9 10 1 Young produced Q_ D D () 6 0 0 0 0 0 Adult mortality '- '-- \.....- '- '- '-- \.... '-- '- '--- 2 Young produced 0 () a 0 0 c 0 a D a Adult mortality

                                                                    '-          \.......       '-            l_         '--            '-                L.             '--          \....     \.......

3 Young produced 0 a 0 D D a 0 a 6 a Adult mortality

                                                                    '-           L...          \......      L             '--            '-             L.                \-         '-- '--

4 Young produced '--\ "'\ '-\ ~ '-\ '-\ '- 5 '-\ Adult mortality L- '-- '-- \..;...

                                                                                                          ,,_             \_            \.....        \.....        '\.....

5 Young produced ~,_ 10 \"L. \'\ l~ 10 lO 10 1\ Adult mortality \.. \.... '-- '- '-- '-- L.. \..._ \..... \...... 6 Young produced (:) 0 c c 0 0 0 0 c c Adult mortality lq L- L '-- l&

                                                                                                                                    ,,\...._

tS

                                                                                                                                                        '--             \......  '-          '---
                                                                                                                                                                                              \t
                                                                                                                                                                     '2*~

7 Young produced IS \c& Total yonng produced

                                                                ~s            '2.~          '"
                                                                                            ~1.          '!>>~
                                                                                                                      *~a.\          J~'-t             :!>\              .4\     ~b            '!>>~

Final Adult Mortality '- \.... '-- '- \..... \.-. '-- '- \.... '-- Nolo: Adult monality (L

  • live, D
  • dead~ SB
  • split brood (single brood split between two days). CO
  • c1111 over 'oflillriruz carried over with aduh durina transfer.

Concentration:

                                                                                                                                 % Mortality:                                              Ci1.

Mean Offsprin~emale: ~&.'\

                                                                                                                                 %Reduction from Control-1:                             *1.07..

CONC: 45.2% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 ("\ 0 0 0 0 0 L 0 a a Adult mortality

                                                                  '-           L-           \.....         '-          \.,_             L                              \.._        L 2         Young produced                      0            0            0               ~          0            0                 0              0            0        0 Adult mortality                     L             L           L           L               '-           '-               L.        \_            L            L 3          Young produced                     0          0             c               0         0               a              a                0            0         0 Adult mortality                     '- l _                  L.             l_          '--           L..                L            \......      L           L-4          Young produced                    '-\           s            '-\.         '-\          ~             "-\                 ~              ~         ~             ~

Adult mortality '\...._ L. \..... \...... \...... L \...... L.. L 5

                                                                                            \.....

n \\ l"b \"Z.. 10 ,,_ Young produced 1\ \"'1.. l() Adult mortality L. \..... '- '-- '--- L \..... \.... L '- 6 Young produced _Q c c 0 0 Q_ 0 c c 0 7 Adult mortality Young produced Total young produced L 2.0 li L '- tl. li{ \Qo

                                                                                                                                       \....

I '"a

                                                                                                                                                      \<<&
                                                                                                                                                                     '~
                                                                                                                                                                                  \....       l....
31. 3"\ ~\ ~~ ~'- 31o ~1. ~ 3"\ 0~

Final Adult Mortality L L. '-- '-- \, Note: Adult mortality (L

  • live, D
  • dead), SB =split brood (single brood split between two days~ CO= cs
                                                                                                                                       '--               '-           \....       '-

over of&orina carried owr with adult durina transler . Concentration:

                                                                                                                                 % Mortality:                                              01.

Mean Offsprin~emale:  !&.b

                                                                                                                                 %Reduction from Control-1:                             ""12..&1.

SOP ATll - Exhibit ATll.2, revision 04-01-09 ~,.age 40 of 105

     ~-
    ~                                                                                                                                                                                Page 4 of7 n

Species: Ceriodauhnia dub/a I~ Client: TVA I Seguoyab Nuclear Plant. OutfalllOl CONe: 72.6% Survival and Reproduction Data Replicate number Date: n-cn.-10 'I I I y;, Day 1 Young produced Adult mortality D 1 D 2 D 3 4 ("')

                                                                                                            \......

5 C)

                                                                                                                          \...-

() 6 L... 0 7

                                                                                                                                                      '--        L 0

8 0 L 9 0 10 2 Young produced ("') 0 0 Q Q_ a_ _Q 0 0 a Adult mortaHty L L- '- '-- '-- '- L.. L.... '-- '-- 3 Young produced 0 0 0 Q c D c _£)_ C) 0 Adult mortality L. L. '- '-- '-- '- L '- '- '-- 4 Young produced Adult mortality "5!. "-\ ~ ~ '- '-\ '-\ '{ ~ ..s

                                                                    '- '-                     \..-           '--         '-               L            L           L..             '--        '--

5 Young produced L"2- tO tb Lo l"L. \'Q 1\ H 'o \~ Adult mortality L. '- '- '- - '- \.... L. '- '-- 6 Young produced D 0 0 c 0 0 0 0 0 0 Adult mortality Young produced ., ,, L '- '-- '--

                                                                                                                       ,,.L....       ~

I&' ,,

                                                                                                                                                    \..._        '--

l\

                                                                                                                                                                                '--         \.....
                                                                                                                                                                                               \'\

7 lt. 2.0 Total young produced

                                                                ~'\            !>\          ~\            ~a           ~\o.          ~~            .:!>1.         ~"\             ~\o         ~1 Final Adult Mortality                             \            '--           '-           '-           ~            '-             '--           '-            '-        \...

Not*: Adult mortality (L

  • five, D
  • dead), SB- split brood (single brood split between two days), CO
  • can over o~ngcarried over with adult durina transfior.

Concentration:

                                                                                                                                 % Mortality:                                              07~

Mean Offspring/Female: ~.~

                                                                                                                                 % Reduction from Control-!:
                                                                                                                                                                                       -* "*'11..

CONC* 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 D D 0 0 0 c a 0 Adult mortality '\....... L- L L L... \...._ \...... L L _l. 2 Young produced 0 c 0 0 D 0 0 0 0 0 Adult mortality L "-- L

                                                                \._
                                                                               '-          L               '-            L.           '--                         L 3         Young produced                   0             a            0             0              ()           D             0             0             0           0*

Adult mortality \._. \...... '- \...- '-- L L \...... '-- L 4 Young produced ~ '-\. ~ s '-\_ ~

                                                                                                                                     \._
                                                                                                                                                    ~             ~              'A           "(

Adult mortality \.- \... \..... '- '-- '-- \,.__

                                                                                                                                                                                 '--           L
                                                                *'4                                                                                             Lo
                                                                                                                                                                                             *~L 5         Young produced                                 \\           \ "L          L'l..       \"1.           \ \           tD                         \1....

Adult mortality

                                                                 '-                \..._       '--           '-          \.....        \.._

6 Young produced c c () c 0 c 0 0 0 0 Adult mortality ~ L \._.

                                                                                                         '-           '-             L..            '- '-                    \,_

i~

                                                                                                                                                                                             '~

2.0

                                                                                             '"            1'1         \'i           1.\             ll\        1'\            11 7         Young produced Total young produced "alit          ~$           ~f..          ~\c
                                                                                                                      ~"
                                                                                                                                     ~,           3'-\          "!Q             ~~            ~Co Final Adult Mortality                             ~            \..        \.             L           '-            L.            L             L               '-          \.._

Not*: Aduk mortality (L*Iive, D = daad), SB

  • spfit brood (single brood split between two days), CO
  • ca over otfsorina carri<d over with aduk durin11 tralllfioi .

Concentration:

                                                                                                                                 % Mortality:                                            Cl1 Mean Offspring/Female:                                *'-<.t
                                                                                                                                 % Reduction from Control-!:                           -2o.l7.

SOP ATil - Exhibit ATl1.2, revision 04-01-09

      \
 .}'age 41 of 105
       .~

ff Page 5 of7

.i.

i Species: Ceriodaphnia dub/a Client: TVA I Seguoyah Nuclear Plant. Outfall lOt CONTROL-2 , Survival and Reproduction Data Date: 1\ *0"1-10 I Day 1 2 Replicate number s 10 1 Young produced a D a 3 0 " c 6 0 c 7 8 _Q_ _Q_ 9 c I 2 Adult mortality Young produced L-6 0

                                                                               \,..

('") 0

                                                                                                                    \...

0 D (')

                                                                                                                                                               \.....

0 0

                                                                                                                                                                                          ~

("') Adult mortality L- L. L L- \..... \.._ \.... L.. \.... \,_ I 3 Young produced Adult mortality D 0 c 0 0

                                                                                                                   \......_

0 L 0 L 0 L... 0 L L. () 4 Young produced '4 _:!) ~ "\ ~ '--\. ~ _"i '-\ ~ I s Adult mortality Young produced JD

                                                                          \!>>
                                                                             '--            \....

l(:) lt:> 1\ lt:;)

                                                                                                                                                \"t L-        '--
                                                                                                                                                                \0
                                                                                                                                                                             \0          I()

Adult mortality L. '-- L- '- '-- '- '-- L- '- '- I 6 Young produced (') 6 c ("') 0 0 0 c 0 0 7 Adult mortality Young produced rS

                                                                 \......   '--
                                                                          \S            ,,
                                                                                                     \'-\
                                                                                                                 ~
                                                                                                                  \'-\          L\o
                                                                                                                                   '--           \,_

h... \'$

                                                                                                                                                               '--          '--          L...

11 I Total young produced Final Adult Mortality

                                                              '2.~
                                                                           ~\
                                                                                        '0\ "28
                                                                                                                   'Ut
                                                                                                                   \
                                                                                                                                 ~c
                                                                                                                                                ~'!.
                                                                                                                                                 \....

2.., 3\ lo.....

                                                                                                                                                                                         ~\

X for 3ra Broods X:: X. )<... >'- ')<... ~  :;><. >'- )<:. ')C. I Nou. Adult monality (L

  • Uve, D =dead), SB
  • split brood (single brood spltt between two days), CO
  • cany over (offspring earned over wtth aduh dunns tranSier).

Concentradon:

                                                                                                                             % Mortality:                                     l C\1 ..

I CONC: 100% Intake Mean Offspring/Female: Survival and Reproduction Data I ~.l Replicate number I Day 1 Young produced 1 0 0 2 D 3 c 4 0 5 0 6 0 7 0 8 a 9 10 0 Adult mortality

                                                                '-        L              L..             L.           L              L             L.          L..            L           L_

I 2 Young* produced Adult mortality 0 0 (}

                                                                                          \....

0 L-D L. L 0 _Q

                                                                                                                                             \......

0 L.. 0

                                                                                                                                                                            '--         L a

3 Young produced 0 D () 0 0 0 0 0 0 C; I 4 Adult mortality Young produced

                                                               "-l L.
                                                                            ~

L "t _\.

                                                                                                      'i s

L L s L

                                                                                                                                                              '5          ~
                                                                                                                                                                           \...._     \.-
                                                                                                                                                                                           ~

Adult mortality \..... L. '-- '-- '-- ""

                                                                                                                                  \.....        '--          '--             '--        '--

I 5 Young produced Adult mortality l\

                                                              \.......
                                                                         \'L
                                                                                         -~
                                                                                                     \ "L I_Q_
                                                                                                                  '--            "'--c 1\
                                                                                                                                                 \.....
                                                                                                                                                             \
                                                                                                                                                                 \         1'0
                                                                                                                                                                                \....

10

                                                                                                                                                                                          \......

6 Young produced c 0 0 c 0 c 0 0 0 I 7 Adult mortality Young produced 11 L L lS

                                                                                           \._        \.....

l't L t'\ L "2.0

                                                                                                                                             \,__

la

                                                                                                                                                             \.......
                                                                                                                                                             \~            LS L         \.......

lR

                                                                                                                                           ~

Total young produced 3t'2.. ~ 2.& ~s ~ ~5 ~\ ~i2. ~ ~ Final Adult Mortality '- \..... '-- \.... \....... Nolt: Adult mortality (L = Uve, D

  • dead), SB
  • sp6t brood (sinal* brood spHt between two days). CO
  • ca
                                                                                                                                 ~               '-            '-            '-

over olliorina carried over with adult durina trans!Or . Concentration: L

                                                                                                                             % Mortality:                                            01.

Mean Offspring/Female: 3t*1

                                                                                                                             %Reduction from Control-2:

SOP ATil - Exhibit ATll.2, revision 04-0 l-09

                                                                                                                                                                                   -'*"1*

,Page 42 ofl05

~

   .ETS TVA I Sequoyah Nuclear Plant, OutfalllOl -Non-treated

~ t: ** November 02-09, 2010 Verification of Cerlodaphnia Reproduction Totals ~ @ --:u.*;~** ...... .,;,_..,'.'*' ~ ~

   !.) Environmental Tesllng Solutions, Inc.

Control-1 72.6% Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 I 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0. 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 3 4 4 4 5 4 4 40 4 5 4 5 5 6 4 4 4 4 5 46 5 11 13 10 13 10 12 II II 10 11 II2 5 12 10 10 13 12 13 11 11 13 13 118 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 15 15 15 14 14 16 13 15 14 15 146 7 17 17 16 20 18 18 17 19 19 19 180 Total 30 32 29 30 28 32 28 31 28 30 298 Total 34 31 31 38 36 35 32 34 ~..]7 .._344. 11.3% 100% Reolicate number Replicate number Day Total Day Total I 2 3 4 5 6 7 8 9 10 I 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 4 4 3 4 5 5 4 42 4 6 4 5 5 4 5 5 6 4 4 48 5 13 10 10 12 11 12 II 13 10 10 112 5 14 11 12 12 12 II 10 13 12 13 120 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 15 l7 16 16 l3 17 15 15 14 16 154 7 19 20 19 19 18 21 19 19 17 19 190 Total 32 32 30 32 28 32 30 33 29 30 308 Total 39 35 36 36 34 37 34 38 33 36 358 22.6o/o Control-2 Re ~Jiicate number Total l Replicate number Day Day Total I 2 3 4 5 6 7 8 9 10 I 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 5 4 4 6 4 5 4 44 4 4 3 4 4 3 4 5 4 4 4 39 5 12 10 12 12 II 13 10 10 10 II III I 5 10 13 10 10 11 10 12 10 10 10 106 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 19 15 16 18 16 17 15 15 15 18 164 7 15 15 17 14 14 16 16 15 17 17 156 Total 35 29 32 35 31 34 31 29 30 33 319 Total 29 31 31 28 28 30 33 29 31 31 301 45.2% 100% Intake RePlicate number Replicate number Day Total Day Total I 2 3 4 5 6 7 8 9 10 I 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 4 6 4 5 5 5 4 46 4 4 4 4 4 5 4 5 5 4 5 44 5 12 11 11 11 12 13 12 10 10 12 114 5 11 12 9 12 10 11 11 11 13 10 110 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 20 18 16 19 18 19 15 15 19 l7 176 7 17 17 15 19 19 20 18 15 15 18 173

         ~T'!!!!..,_         36     34        31 34     36      36  32 30   34   33    336            Total   32 33  28 35 34 35 34         31 32  33     327

~ ~ TVA I Sequoyah Nuclear Plant, OutfalllOl ol::.. ol::.. Non-treated ~ November 02-09,2010 ~

  • Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test
  • ET~

EPA-821-R-02-013, Method 1002.0 Quality Control

       ~      Environmental Testing Solutions, Inc.

Verification of Data Entry, Calculations, and Statistical Analyses Project number~ 6709 Keve*weo oy: ~"v~ Concentration Replicate number Survival Average reproduction Coeftlcleat of Percent reductloa fram (*~) (%) (offspring/female) nrlotloa (%) control (%) I 2 3 4 5 6 7 8 9 10 Control-! 30 32 29 30 28 32 28 31 28 30 100 29.8 5.2 Not applicable ll.3% 32 32 30 32 28 32 30 33 29 30 100 30.8 5.3 -3.4 22.6% 35 29 32 35 31 34 31 29 30 33 100 31.9 7.2 -7.0 45.2% 36 34 31 34 36 36 32 30 34 33 100 33.6 6.3 -12.8 72.6% 34 31 31 38 36 35 32 34 36 37 100 34.4 7.1 -15.4  ! 100% 39 35 36 36 34 37 34 38 33 36 100 35.8 5.2 -20.1 Ii Control-2 29 31 31 28 28 30 33 29 31 31 100 30.1 5.3 Not applicable 100% Intake 32 33 28 35 34 35 34 31 32 33 100 32.7 6.5 -8.6 OutfalllOI: MSD= Minimum Significant Difference Dunnett's MSD value: 2.057 PMSD= Percent Minimum Significant Difference PMSD: 69 PMSD is a measure of test precision. The PMSD is the minimum percent difference between tbe control and treatment tbat can be declared statistically significant in a whole effluent toxicity test. Intake: Dunnett's MSD value: 1.451 Lower PMSD bound determined by USEPA (l<f' percentile) = 13%. PMSD: 4.8 Upper PMSD bound determined by USEPA (91f' percentile)= 47%. Lower and upper PMSD bounds were determined from tbe 10tb and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200Ia; USEPA, 200lb). USEPA 2000. Understanding and Accounting for Metbod Variability in Whole Effluent Toxicity Applications Under tbe National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Cbrooic and Acute Whole Effluent Toxicity Test Metbods, Volumes 1 and 2-Append.ix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

~ ~ TVA/ Sequoyah Nuclear Plant, Outfall 101 ~ Non-treated

     *Ers

~ November 02-09, 2010 ~ Statistical Analyses

     ~      ""'".:;,;/ ~1       ,_.,,      **,f'<;,-
     ;,) Environmental Testing Solutions,             .,c.

Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: ll/212010 TestiD: CdFRCR Sample 10: TVA I Sequoyah Nuclear Plant, OutfalllOl End Date: ll/912010 LabiD: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Cone-% 1 2 3 4 5 6 7 8 9 10 Control-! 30.000 32.000 29.000 30.000 28.000 32.000 28.000 31.000 28.000 30.000 Control-2 29.000 31.000 31.000 28.000 28.000 30.000 33.000 29.000 31.000 31.000 11.3 32.000 32.000 30.000 32.000 28.000 32.000 30.000 33.000 29.000 30.000 22.6 35.000 29.000 32.000 35.000 31.000 34.000 31.000 29.000 30.000 33.000 45.2 36.000 34.000 31.000 34.000 36.000 36.000 32.000 30.000 34.000 33.000 72.6 34.000 31.000 31.000 38.000 36.000 35.000 32.000 34.000 36.000 37.000 100 39.000 35.000 36.000 36.000 34.000 37.000 34.000 38.000 33.000 36.000 Intake 32.000 33.000 28.000 35.000 34.000 35.000 34.000 31.000 32.000 33.000 Transform: Untransformed  !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t.Stat Critical MSD Mean N-Mean Control-! 29.800 0.9900 29.800 28.000 32.000 5.199 10 32.717 1.0000 Control-2 30.100 1.0000 30.100 28.000 33.000 5.299 10 11.3 30.800 1.0233 30.800 28.000 33.000 5.258 10 -1.112 2.287 2.057 32.717 1.0000 22.6 31.900 1.0598 31.900 29.000 35.000 7.156 10 -2.334 2.287 2.057 32.717 1.0000 45.2 33.600 1.1163 33.600 30.000 36.000 6.306 10 -4.224 2.287 2.057 32.717 1.0000 72.6 34.400 Ll429 34.400 31.000 38.000 7.147 10 -5.ll3 2.287 2.057 32.717 1.0000 100 35.800 Ll894 35.800 33.000 39.000 5.234 10 -6.670 2.287 2.057 32.717 1.0000 Intake 32.700 1.0864 32.700 28.000 35.000 6.455 10 Auxiliary_ Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.720721424 1.035 -0.072330161 -0.95920896 Bartlett's Test indicates equal variances (p = 0. 71) 2.931964874 15.08627224 The control means are not significantly different (p = 0.67) 0.426640917 2.100922029 Hypothesis Test (!-tail, 0.05) N()EC WEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 2.057058738 0.069028817 51.93666667 4.046296296 3.IE-08 5, 54 Treatments vs Control-I Linear Interpolation (200 Resamples) Point  % so 95%CL Skew ICOS >100 IC10 >100 ICI5 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqnl01_110210dauz

~ ~ TVA I Sequoyah Nuclear Plant, OutfalllOl - Intake Q\ Non-treated ~ ~ t.JJ November 02-09, 2010

     .ETS
     ~
           '"-.'.<*:;/".;;,      'i*_,,..

Environmental Testing Solutions, Inc.

                                                 ... :~'""

Statistical Analyses CeriodaJ!Imia Survival and Reproduction Test-R9!roduction Start Date: 111212010 TestiD: CdFRCR SampleiD: TVA I Sequoyah Nuclear Plant, Intake End Date: 11/9/2010 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Cone-% I 2 3 4 5 6 7 8 9 10 Control-I 30.000 32.000 29.000 30.000 28.000 32.000 28.000 31.000 28.000 30.000 Control-2 29.000 31.000 31.000 28.000 28.000 30.000 33.000 29.000 31.000 31.000 11.3 32.000 32.000 30.000 32.000 28.000 32.000 30.000 33.000 29.000 30.000 22.6 35.000 29.000 32.000 35.000 31.000 34.000 31.000 29.000 30.000 33.000 45.2 36.000 34.000 31.000 34.000 36.000 36.000 32.000 30.000 34.000 33.000 72.6 34.000 31.000 31.000 38.000 36.000 35.000 32.000 34.000 36.000 37.000 100 39.000 35.000 36.000 36.000 34.000 37.000 34.000 38.000 33.000 36.000 Intake 32.000 33.000 28.000 35.000 34.000 35.000 34.000 31.000 32.000 33.000 Transfonn: Untransfonned 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-I 29.800 0.9900 29.800 28.000 32.000 5.199 10 Control-2 30.100 1.0000 30.100 28.000 33.000 5.299 10 I 1.3 30.800 1.0233 30.800 28.000 33.000 5.258 10 22.6 31.900 1.0598 31.900 29.000 35.000 7.156 10 45.2 33.600 1.1163 33.600 30.000 36.000 6.306 10 72.6 34.400 1.1429 34.400 31.000 38.000 7.147 10 100 35.800 1.1894 35.800 33.000 39.000 5.234 10 Intake 32.700 1.0864 32.700 28.000 35.000 6.455 10 -3.108 1.734 1.451 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01) 0.954421401 0.868 -0.721765822 0.887179168 F-Test indicates equal variances (p = 0.42) 1.751091719 6.541089535 The control means are not signi_!icantly different (p = 0.§]) 0.426640917 2.100922029 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.450821719 0.048200057 33.8 3.5 0.006077496 I, 18 Treatments vs Control-2 sqnl01_1102JO~a

~ TVA I Sequoyah Nuclear Plant, OutfalllOl - Non-treated ~ November 02-09, 2010 ~ ~ ~

v.
  • Ceriodaphnia dubia Chronic Whole Emuent Toxicity Test
    ~
    ~

ETS  !.'"*:7\ J.: <;::...:>:**.:*.,."**;:,,. Environmental Testing Solutions, Inc. EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Project number: 6709 Control 11.3% 11.6% 45.1% 71.6% 100% 100% Iutake Alkalinity (mgtL L.:aL.:U3) Harduess (mg/L CaC03)

                       --     --    - ---    -                      -    ~

r . iE.~"~'" T.cS""""'

                  ,.,:}
                  ~
                        .. -*-        *-*      *~*,.

EmrlranmenttiTIICfngSalutlons,lnc. Page 6 of7 Species: Cer/odaphnla dub/a Client: TVA I Sequoyah Nuclear Plant. OutfalllOl CONTROL r. i r

  • I 11.3%

22.6% I 45.2% I I 72.6% I I 100% 100% Intake SOP ATll - Exhibit ATll.2, revision 04-01-09 age48 of lOS

              ~ETS Page 7 of7
              ~ !nllllunmtfthiTntlngSalutfons.lnc.

Species: Ceriodanhnia dub/a Client: TVA I Seguoyab Nuclear Plant, OutfalllOl Date: u~<D.*IO CONTROL 11.3%. 22.6% I 45.2% 72.6o/o I I 100% 100% Intake SOP AT II - Exhibit ATI I.2, revision 04-0 I-09 Page 49 of 105

I

  • Page l of6
         ~

Chronic Whole Effiuent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimepha/es promelas Client: Tennessee Valley Authority County: !Y!Y Facility: Seauoyah Nuclear Plant Outfall: 101 NPDES #: TN0020168 Project #: _....;:'-~1LlD.._~_.___ _ __ Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Each concentration was UV -treated Effluent volume (mL) 282.5 565 1130 1815 2500 for 2 minutes to remove pathogenic Diluent volume (mL) 2217.5 1935 1370 685 0 Interferences. Total volume (mL) 2500 2500 2500 2500 2500 I Test orflanism information: Test information: Organism age: \~.4S."'\ 1-\ft" Mo. () \.b Randomizing template: f)l~e.. I Date and times organisms were born between: I\'"(),., 0 lloOO Incubator number and shelf location: '

                                                                                                                                ~

Organism source: A~ &\*tu4 Pr &\"0\*IC Artemia CHM number: C.~t\31\ I Drying information for weight determination: Transfer bowl information: pH= 1.1, s.u. Date I Time in oven: 11-Nl*IO IllS I Average transfer volume: Temperature =15.'1.!'C . o.'~'N......O. Initial oven temperature: !Db. t. Date I Time out of oven: 11-rb*IO Final oven temperature: '-(. I Daily feeding and renewal information: Total drying time: 1.~*lo\bd~ Day Date Morning reeding Test initiation, Sample numbers used I Afternoon reeding renewal, or MHSW batch I I I I I I IPage 50 of 105 SOP AT20 - Exhibit AT20.3, revision 04-01-09

Page 2 of6

              .;) Environmental Testing Solutions, Inc.

Species: Pimephales promelas , Client: TVA I SeQuoyah Nuclear Plant, Outfall101. UV-treated Date: 1\*0t*IO Survival and ,..._ *'- Data Day CONTROL 11.3% 22.6% UV-treated A B c D E F G H I J K L 0 ID ID to ID IO ID 10 10 ID 10 '0 10 1

                                                                  /0           IO        /0       I{)     IO     10     /0     10 IO         I~     10       10 2                                                       '

IO IC /Q /Q IC I(J to /0 IO /Cj 10 10 3 lC IC It:> IO 10 lO 10 IO lt> 10 it) 10 4 10 /0 10 10 10 tO /0 to rc I() /Q fQ 5 I0 IC f() 10 10 lO 10 IQ tO tD 10 ID 6 IO I'D LO 10 IC 10 10 IO lC It> 10 10 7 10 r'() /l) IQ *o 10 IC to /0 IO 10 tO' A= ~::.!e!~!~~j~ W.u.t

                                                                                ,_.'\0 \'-\!\1 ,,..,~ \?~~ ~~.-1\t \?a .'tl .\~tM ~,..~" I\~~ \'\.fb\ 1\1).~
                           -=rw.~                              ~?*"'

[

                                              'L.lO
  • 'I B ' Pan+ ~an:ae weight j~ 'f
                            ~:::;sn .n. ao C = Larvae weight (mg)
                            =B-A rzt.Cft ~--*-

1t\.f;t' lal15 Zf.'PC 21-=l-\ ~ lll,l~ 111-!H llh_ld I7J.'I~ ~'1.'7 ~~ 1*"-1 ,,\.() &,7..\ '\.10 &.14\ '\,oR &.l.\. &.!tb &.2.1 a.c.t ~*Sl [1.\\ iu Weight per initial number oflarvae (mg)

 !~
                                                                                                                                               \o~
                                                                                                                                                     ~       "~

o*r# Cl~ 0~~

                                                                                        .c)       ~f:J                                 ,~
                            = C I Initial Dllmber of
                                                                  ~
                                                                            ,...'-'0                      ~                          ~
                                                                                                                                             ~

(). lo~ larvae b. Cl. (:)' t>' r:;,* lo' ()" Avenge *Percent

~ weight per initial reduction from o.&-o"l o. &Ia\ ~ z.&7.. o.~~ -o.~"t..

number of control(%) J!n'!elmg)

! 1:

u Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Calculations and data reviewed: ~ u IJ ICommmU: I SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 51 of 105

r1 ..ETS i" Page 3 of6

  ~--       : ,. _,* " -" "   *'+     ;"*-,***':*.
  *-*       ~ Environmental lUting Solutlans, Inc.

~ I Species: Pimephales promelqs Client: TVA I Seauoyah Nuclear Plant. Outfall101. UV-treated Date: t \-dl.*IO I Day 45.2% Survival and Growth Data 72.6% 100% M N 0 p Q R s T u v w X I 0

                                                           /D       lb ID            /Q      /Q        'D ID           /D         /0         /0        10 /0 l
                                                                                                                                                       /0 I                                   2 10 to I()

10 lb IO 10

                                                                                    /b
                                                                                             /0 lb
                                                                                                      /0 I()
                                                                                                             /()

10 10 10 I()

                                                                                                                                   /()

10 10 IO 10

                                                                                                                                                                  /Q I                                   3 4

ID I() IC> t() ID /0 I() tO fD I() /C lt)

                                                          /D       It>    /0        lO      tO       10     IC        10           LC       10       'C          10 I                                   5 It>      lC       lO       ID     lt>        II.) IQ         lO          /Q       IC        to          /{)

6

                                                         /D       (Q                        IC       /()     IC         IQ          ID 10              I(J        10 I                                   7 ID      to 10 rc LO IC      l()     IO      /()       to         IO         IO       10          10 ft!

I A= Pan welgbt (~ Tray color;;;e::,~\At Analyst: ~eM tV l4J.\" ~.f) ~::\") \'t.\~ \"\.~ ~"\~ ~fb\ \'?~ \~.'\1 \'i.\'\ Date: \CfM. .\tl I 8 = Pan + Larvae weight (mg) Analyst* '(" Date: \l* \\

  • l D 2tfU 'Z.\'(=1 z.a.tt5 tz.\~ z;z.ctt; 1};t;'6 22-Cf~ L'1.0l ~1-2 1tt3 23::1-1 V(,'IJ, C "' Larvae weigbt (mg)

I "'8-A S.'~1 '\.~ &.'\1 a.!M &o'LO &.'tO. \*~"' '\.~1. a.'t\ l.I)C) ~.1.'\ '\.cr'\ I Weight per initial number of larvae (mg)

                                                         ~~        ~      ~  "      ~)...
                                                                                           "'~      ~
                                                                                                      ~      {/:'~     K '-'"'     ~          ~'t>
                                                                                                                                                     ,._,..,      f:lt..t-
                  = C I Initial number of larvae                  ~                ~

0' o* o* o* ~* o* o* o* t1 b' ()* I ~* I Average weight per initial Percent reduction from control 0 .t'-1 - !>>.I.'t o. tlo"\ *'!t."Z.7. o. &&.2. -~.l7. number of (%) I larvae (me:) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.

  • I Calculations and data reviewed: J(__

Comments: I I I Page 52 of 105 SOP AT20- Exhibit AT20.3, revision 04-0 l-09

Page 4 of6

         £ETS
         ~  Envlronmentol Tostlng Solution., Inc.

Species: Pimephales urome/as Client: TVA I Seguoyah Nuclear Plant, OutfalllOl. UV-treated Date: 1\:cn. *to Day 0 1

                                                              /0 /0   10 2

(0 ID (() 10 3 (0 /0 lQ IO 4

                                                     'D     10   10  10 5

to /fl /C 6 ID I() IO 7 10 (() /Q I II I per oflarvae (mg) I = C l Initial number of larvae Average weigbt per reduction I initial number or larvae from control (%) 0* &'\ \ I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm =unusually small, lg =unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: L I I I IPage 53 of 105 SOP AT20 -Exhibit AT20.3, revision 04-01-09

~ ~ ~ TVA I Sequoyah Nuclear Plant, OutfalllOl UV-treated ~ ~ November 02-09,2010 v., Pimephales promelas Cbronic Wbole Effluent Toxicity Test EPA-821-R-02-013, Metbod 1000.0 e Quality Control Verification of Data Entry, Calculations, and Statistical Analyses 0 Environmental Testing Solutions, Inc. Project aamber: r' 11~

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A 10 10 13.41 21.98 8.57 0.857 0.857 Coaln>l B 10 10 12.90 20.50 7.60 0.760 0.837 7.5 0.760 100.0 8.837 7.5 Not applicable c 10 10 14.92 23.13 8.21 0.821 0.821 D 10 10 12.88 21.98 9.10 0.910 0.910 E 10 10 13.92 22.71 8.79 0.879 0.879 F 10 10 13.56 22.64 9.08 0.908 0.908 11.3% 0.861 4.6 100.0 11.861 4.6 -%.8 G 10 10 13.92 22.18 8.26 0.826 0.826 H 10 10 13.04 21.34 8.30 0.830 0.830 I 10 10 12.59 20.87 8.28 0.828 0.828 J 10 10 13.26 21.94 8.68 0.868 0.845 0.868 12.6% 10.8 100.0 11.845 10.8 -11.9 K 10 10 12.81 22.32 9.51 0.951 0.951 L 10 10 15.06 22.37 7.31 0.731 0.731 M 10 10 13.54 21.91 8.37 0.837 0.837 N 10 10 14.46 23.47 9.01 0.901 0.901 45.2% 0.867 4.2 100.0 11.867 4.2 -3.6 0 10 10 14.46 23.43 8.97 0.897 0.897 p 10 10 13.85 22.19 8.34 0.834 0.834 (j 10 10 14.75 22.95 8.20 0.820 0.820 R 10 10 14.29 22.58 8.29 0.829 0.829 72,6% 0.864 7.0 100.0 0.864 71J -3.2 s 10 10 14.43 22.97 8.54 0.854 0.854 T 10 10 14.54 24.06 9.52 0.952 0.952 u 10 10 13.81 22.72 8.91 0.891 0.891 100% v 10 10 13.73 21.73 8.00 0.800 0.882 6.4 0.800 100.0 11.882 6.4 -5.3 w 10 10 14.42 23.71 9.29 0.929 0.929 X 10 10 15.19 24.26 9.07 0.907 0.907 y 10 10 14.20 22.99 8.79 0.879 0.879 IOU"~ lutake z 10 10 1325 22.01 8.76 0.876 0.891 3.3 0.876 100.0 0.891 3.3 -6.5 AA 10 10 13.22 21.97 8.75 0.875 0.875 DB 10 10 13.74 23.09 9.35 0.935 0.935 - ..... - OpJfa!IIOJ: MSD* Minimum SignifiCIIIll Difference Da*aett'a MSD val*e: 8.1035 PMSD* Percent Minimum Significant Difference PMSD: 11.4 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole eftluent toxicity test Ia take: Dauett'* MSD value: 0.0675 Lower PMSD bound determined by USEPA (I Oth percentile) = 12%. PMSD: 8.1 Upper PMSD bound detennined by USEPA (90th percentile)= 30'~. Lower and uppe< PMSD bounds were determined from the lOth and 90th percentile, respeetively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200la; USEPA, 200lb). USEPA. 2001 a, 2001 b. Final Report: Jnterlaboratooy Variability Study of EPA Short-term Chronic and Acute Whole.Effiuent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821 -B-01-004 and EPA-821-B-01 -005. US Environmental Protection Agency, Cincinnat~ OH.

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Larval Fish Growth and Survival Test-7 D!!I Growth Start Date: 111212010 TestiD: PpFRCR SampleiD: TVA I Sequoyah Nuclear Plant, Outfall I 01 End Date: 11/9/2010 LabiD: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-Treated Cone-% I 2 3 4 D-Control 0.8570 0.7600 0.8210 0.9100 I 1.3 0.8790 0.9080 0.8260 0.8300 22.6 0.8280 0.8680 0.9510 0.7310 45.2 0.8370 0.9010 0.8970 0.8340 72.6 0.8200 0.8290 0.8540 0.9520 100 0.8910 0.8000 0.9290 0.9070 Intake 0.8790 0.8760 0.8750 0.9350 Transform: Untransformed  !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.8370 1.0000 0.8370 0.7600 0.9100 7.529 4 0.8592 1.0000 11.3 0.8608 1.0284 0.8608 0.8260 0.9080 4.608 4 -0.553 2.410 0.1035 0.8592 1.0000 22.6 0.8445 1.0090 0.8445 0.7310 0.9510 10.820 4 -0.175 2.410 0.1035 0.8592 1.0000 45.2 0.8673 1.0361 0.8673 0.8340 0.9010 4.234 4 -0.705 2.410 0.1035 0.8592 1.0000 72.6 0.8638 1.0320 0.8638 0.8200 0.9520 7.012 4 -0.623 2.410 0.1035 0.8592 1.0000 100 0.8818 1.0535 0.8818 0.8000 0.9290 6.428 4 -1.042 2.410 0.1035 0.8592 1.0000 Intake 0.8913 1.0648 0.8913 0.8750 0.9350 3.278 4 Auxili!!!l: Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98219049 0.884 -0.040311724 -0.1382183 Bartlett's Test indicates ~ual variances~= 0.71~ 2.94596696 15.0862722 H~thesis Test ~1-tail, 0.052 NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 I 0.10347888 0.12363068 0.00104427 0.00368722 0.91621083 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point  % SD 95% CL(Exp) Skew

     !COS                     >iOO IC10                       >100 ICI5                       >100 IC20                       >100 IC25                       >100 IC40                       >100 IC50                       >100 sqn101_110210data

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Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11/2/2010 TestiD: PpFRCR SampleiD: TVA I Sequoyah Nuclear Plant, Intake End Date: 11/9/2010 LabiD: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Conunents: UV-treated Cone-% I 2 3 4 D-Control 0.8570 0.7600 0.8210 0.9100 11.3 0.8790 0.9080 0.8260 0.8300 22.6 0.8280 0.8680 0.9510 0.7310 45.2 0.8370 0.9010 0.8970 0.8340 72.6 0.8200 0.8290 0.8540 0.9520 100 0.8910 0.8000 0.9290 0.9070 Intake 0.8790 0.8760 0.8750 0.9350 Transform: Untransformed 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.8370 1.0000 0.8370 0.7600 0.9100 7.529 4 11.3 0.8608 1.0284 0.8608 0.8260 0.9080 4.608 4 22.6 0.8445 1.0090 0.8445 0.7310 0.9510 10.820 4 45.2 0.8673 1.0361 0.8673 0.8340 0.9010 4.234 4 72.6 0.8638 1.0320 0.8638 0.8200 0.9520 7.012 4 100 0.8818 1.0535 0.8818 0.8000 0.9290 6.428 4 Intake 0.8913 1.0648 0.8913 0.8750 0.9350 3.278 4 -1.562 1.943 0.0675 Auxili!!!1 Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93294114 0.749 0.02117318 0.51937165 F-Test indicates !:jUal variances !2 = 0.24) 4.65254307 47.4672279 Hn!2thesis Test ~1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.06748823 0.0806311 0.00588612 0.00241246 0.16931061 1, 6 Treatments vs D-Control sqnl 01_1102 /Odata

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Pimephales promelas Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number: 6709 Coneentration Control 11.3% 22.6% 45.2*/a 72.6% 100% 100% Intake lkalinity (mgtL LaLU:J) b4 Hardaess (mg/L CaCO:J) 72

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Page 5 of6 I Species: Pimepha/es prome/as Client: TVA I Seauoyah Nuclear Plant. OutfalllOl. UV-treated Date: __,li..Ll,..,.<n.._*...,IDe--_ __ I I I CONTROL UV-treated I I 11.3% I 22.6% I I 4S.Z% I 72.6% I I 100% I I I 100% Intake I IPage 58 of 105 SOP AT20- Exhibit AT20.3, revision 04-01-09

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Environmental Testing Salution-.lnc. l Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L n II Date analyzed Analyst Iu* ~

                                                       .I 0   -I Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent:

Acid reagent: IWj{g L \'2...

                                                                                                                                                             \lo L aboratory controI stand,ar.d.:                                        '

Reference standard True value (TV) Meas~red value (MV) %RS=MV/TVx 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) lJ\J SS':l:_(uCQ o.so U.')l\ lO 2.. 'J-"t. Sample characteristics lI

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Note: All samples were analyzed in excess recommended holding time (lS minutes) unless otherwise noted. True value (TV)  % RS = MV I TV x 100 (mg!L) (acceptable range= 90 to 110%) 0.50 Reviewed by r--:';%1~r,....:-:--~ Date reviewed .___...;.lFC_~

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Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Analyst Date analyzed In. ~ I I0 Meter: Accumet Model AR25 pH/Ion Meter I\ Iodide reagent: N~ Uo Acid reagent: \ W~ ~ t\\ Calibration: Laborato control stantlard: Reference standard True value (TV) (mg!L) 0.50 Laborato control standard: Reference standard True value (TV) Measured value (MV) number (mg!L) (mg/L) IN ,to&' 0.50 o. Reviewed by ~-"i:J~,..,...~-~ Date reviewed ~.._.::.;IJ.i;..*_o_q;..*..:.f,,.o~...... IPage 61 of 105 SOP C8 - Exhibit CS.I, revision 09-01-09

Page 65. Page~o{7

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Environmental Testing Solutlanr,lnc. Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pH/Ion Meter IS: Date: In.fl 10 Iodide reagent: Acid reagent: ~

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Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L I Analyst ~\\{ Date analyzed \ : 0 (&, * \ 0 Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent: Acid reagent:

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Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Date analyzed \ l* 0 I M!' t . Q Iodide reagent: Acid reagent: I::::=---:...... ~ I of the samples. Laboratory controltd.rd s an a : ' Reference standard True value (TV) Measured value (MV)  % RS = MV I TV x 100 number (mg!L) (mg/L) (acceptable range= 90 to llO%) 1:.\-...)\\ 1(o~ 0.50 0.4-t':>t{ qt,S"L Sample characteristics I a ratory controI stan a : Lbo Reference standard True value (TV) Measured value (MV)  % RS = MV I TV x 100 I number (mg!L) u ...v (mg!L) (acceptable range= 90 to 110%) TVI1 I  ::If I Reviewed by Date reviewed ._1-_-_,-,:,.:~:...

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Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Titrate samples to pH= 4.5 S.U. Titrant normality and multiJJiler determination: .*-..,\.* .. """'"" ~ pH of Normality Normality (N) ofH1S0 4 pH Factor orMitlii()iier* Deionized Titrant check Begin End Total = (5 ml Na1C03 x 0.05)/E = (Nx 50000)/100 nil sample water reference standard ml ml ml =0.25/E =NxSOO

     =4.SS.U.       number          number                         (E)     (acceptable range= 0.0180
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Reference standard True value Sample I' Alkalinity (MV) o/o RS= MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaCO,VL) (ml) ml ml ml =90 to 110%) I~'SSPRil 100 100 IL*~ ~0.1 q,_3 ln.~ /00 (6b,D'/ Sample Alkalinity o/oRPD = Sample ID volume Total Multiplier (mg CaCO,VL) {(S *D) /[(S+D)/2)} x 100 (acceptable range= :1: 10%) J Duplicate (B) atrtx spt e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCDJIL) (ma CaCOJIL) (ml) ml ml ml IN~eao ffi) I()D ~\1.0 3:2{) e..o 1o.e e(p 0 Sample alkalinity (B) Measured spike value (MV) /o R=MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mr CaCOJIL) =75 to 125%)

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          ~of                                       Normality                                           Normality (N) of H 2S04               pH Factor or Multiplier Titrant                 check        Begin        End     Total        = (5 ml Na2C03 x 0.05)/E            = (N x 50000)/ 100 ml sample 0      Kefe~~nce                 standard          ml          ml       ml                 =0.25/E                                 =Nx500
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Page _ __ Page ---.:;__of 5 Alkalinity (SM 2320 B) Analyst Date analyzed I ~St.. II* o 1- ,Q Matrix: Water, RL = 1.0 mg CaC03/L Titrate samples to Time initiated ~--.!.\.:..:\Q~C;,__ _---1 Time completed .....___;.1-3....:.;:o:;...~----' pH= 4.5 S.U. Titrant normali pH of Normality Normality (N) ofH1S04 pH Factor or Multiplier Deionized Titrant check Begin End Total = (S ml Na1C03 x 0.05)/E = (N x 50000)/100 ml sample water reference standard ml ml ml = 0.25/E =NxSOO

             = 4.5 S.U.           number                number                                  (acceptable range= 0.0180 - 0.0220) 0*020 Reference standard                 True value          Sample                                                      Alkalinity (MV)       % RS= MV /TVx 100 number                           (TV)         volunie     Begin     End      Total      Multiplier         (mg CaCO:YL)              (acceptable range (mg CaCO:YL)             ml          ml      ml       in I                                                     = 90 to 110°/o) 100            100 12.1>    21.                                                                       oJ ..

Alkalinity %RPD= Multiplier (mg CaCO:YL) {(S- D) /((S+D)/21} x 100 (acceptable range= :1:: 10%) s D Spike alkalinity (A) Begin End Total Multiplier (mg CaCO:YL) ml ml ml lOO .2.o.J..? 3.< .5 ro.o \t> .4-Sample alkalinity (B) Measured spike value (MV)  % R=MV /SVx 100 (mg CaCO:YL) MV=A-B (acceptable range (mR CaCQ:YL) =75 to 125%) 5? 5o \Cocfo samp,l e measurements: I Sample number Sample ID Sample volume

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Begin ml End ml Total ml Multiplier Alkalinity _(mg CaCO:YL) ll-02..(0 ~~ ".,0 \DO* oz.~ ~~.s !Q.O \o.£4- lo2 I \\ ._()'5. LD l\*01. LO I

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Page _2....,___ Page Z.. of_,S=--- Alkalinity (SM 2320 B) Matrix: Water, RL = l.O mg CaC03/L Analyst ~-~fDL.~"'------; Time initiated I ....... bate analyzed (1. 01- *tO Titrate samples to pH= 4.5 S.U. Time completed ~=====.......:;======= Normality (N) of H 2S04 pH Factor or Multiplier

                                                                                            = (5 ml Na2C03 x 0.05)/E               = (N x 50000)1 100 ml sample
                                                                                                     =0.15/E                                 =Nx500
                                                                                                          =0.0180-True value         Sample                                                 Alkalinity (MV)        % RS= MV /TV x 100 number                  (TV)          -volume     Begin      End      Total    Multiplier       (mg CaCOJIL)              (acceptable range (mg CaCOJIL)            ml       ml       ml         ml                                                  =90 to 110%)

100 100 ,q.o ,o.<+ qq l:tttOfo

                                                                      '\.'5 Sample                                                    Alkalinity                  *,t,RPD=

volume Begin End Total Multiplier (mg CaCOJIL) {(S *D) /((S+D)/2)} x 100 ml range= :1: 10%)

                                                                               ~-~       3. *"      leA-
                                                                              ~5.1-
                                                                                         ;.-;        .l-M at.riX smike recoverv:

Reference standard Spike value Sample Spike alkalinity (A) I number (SV) (mg CaCOJIL) volume (ml) Begin ml End ml Total ml Multiplier (mg CaCOJIL) t!:vv~<. 't ~'D '9) J()) a~.LJ. ~o.ft1 <o.2. IDH B'5 I Sample alkalinity (B) (mg CaCOJIL) Measured spike value (MV) MV=A-B

                                                                                                                      % R= MV /SVx 100 (acceptable range I                                                                                   (mg CaCOJIL)                         =75 to 125%)

3li- 151 I 02.Pio sam_]ljl e measurements: I Sample number Sample ID Sample volume (ml) Begin ml End ml Total ml Multiplier Alkalinity (mK CaCOJIL) loll o~. "Z..

  • Soru11oO~. z.. \l)D 30.1.. 34.~ &.1- IO.Y. 3i I J.D u_nc;. .u-4 1 bll 01..0 I C'nO_iLll-l l 3 a,<;
                                                                           \           :1,4.~

01-.q 4t2. 31.ct 3.tt,

                                                                                                           ;,;      y) 31-1'-10 z.'

I 10 llo'l. o\ Ju I I 0 I. .(1!) I I 3 \ IJfL2, #.4

                                                                                     ~-q.,       ~'+                                                                                                              3.2 3.~

I t3u ILK) I~ II ol.ot.l --rJ A- PAt: i"x J 6D b,O 3.q (2) ~I Ii I 0 II n~. '2.~ I I

z. 3.'1 Q.J
                                                                                                            ~B J.+:z,                             S-:f 10 11    nc.o'\                                3                         9.1
                                                                                                 '~-'      1-\-.v                             93

~ ..i IOIIOI.oS "f(A fA,:' II'ST c Reviewed by:

                                                                                       ~-'       \1-\
                                                                                                     ~t'A-t:;.o             . ..-

Date reviewed:

                                                                                                                                             \0\)

Iu.oJ.w

                                                                                                                                                       ,. 10'-1

~age 69 of I 05 SOP C6- Exhibit C6.1, revision 09-0 l-09

Page-=-- Page 3 of~5=--- Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCOJIL Analyst ~c&s:;z<~...._----1 Time initiated ~.............

                                                                                                                                                           -=-....,....----1 Date analyzed      II* 0-:f*lO                                Titrate samples to                             Time completed .                 ~

pH=4.5 S.U.

                . Titrant normality an d mu[I'll   ttp4 er determmatton:

otto! Normality Normality (N) ofHzS04 pH Factor or Multiplier Deionized Titrant Ranln End Total = (5 ml NazC03 x 0.05)/E = (N x 50000)1 100 ml sample water reference standard ml m1 =0.25/E =Nx500 number (~an ..e- 0.0180

  • 0.0220)
                  -4.5S.U.       number                                               (E)
             .:La oratory contro s an at'< :                                                                                                                        -

Reference standard True value Sample ' Alkalinity (MV) o/o RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mgCaCOYf,) (acceptable range (mgCaCOJIL) (ml) ml ml ml =90to 110%)

1:'-NSS~o 100 100 n*.l ~~.) 'l!\. \C.~ q"5 cts"/o Alkalinity o/oRPD =

Multiplier (mg CaCOYf,) {(S *D) /[(S+D)/2)} x 100 (acceptable range =:I: 10%) M.atl"lx spl'ke recovery: Reference standard . Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mgCaC<M.) (meCaCOJILl (ml) ml ml ml i:t"NSS &?>"D lDO 9) .;1.1- ~-~ 10.0 ('l) li:>.Lf 2.\0 Sample alkalinity (B) Measured spike value (MV) o/o R = MV I SV x 100 (mgCaCOJIL) MV=A-B (acceptable range (m2CaC(M.l =75 to 125%)

                                                            \\0                                   Jet.                                 lt>octo samo,[ie measurements:

Sample volume Begin End Total Alkalinity Sample number SampleiD (ml) ml ml ml Multiplier (mzCaCOJIL) ll\ II OS. lO 1-"'QAPAF lNf 3 5D ~\-1' J."to~ 5'.'2- IN\ lo.~ 1'o -tua 10 Ill\~ 1"1 II'A~~Cb2 AA 0.0 5'-0 s.v fOO . ,()"\ I0 Jl 01!.. '2i

                                                           \t.if c:tA                          5.0      I\t) *'        ~.I                                 110          . ,o..

I oil 0 ~ l.'S oo*L f.tP It> .I 15.o ,..._q too *lb2 J 0 I I 0~ * '1.4 .1NTAP In o IJo~ I=) .tl. 1r~

  • tl2.
                                     \1A .s&N tD\                            ~00              .2n.c.l- :~~.Lt                    (t)                      w J 0 1\0l .tn.

1 oil 0"!..2.1 I 2 ' ~~\p l~.o

                                                                                                                     ~.2
                                                                                                                       ~.4                                 ":},

(,1-10 11os. en ~ 3 !a1-.o 30.3 a..:s l#CJ 1011 oi.Q'!, ~A5Qil )1\\"\ ' Revtewed by: 1~.3 ~~.'5 3:2-Date reviewed:

                                                                                                                                                          ~ -((:J I    \l. cr:J-10
  • age 70 of 105 SOP C6- Exhibit C6.1, revision 09-01-09

Page _Y.._,___ Page t+ of-.:5::___

           )  Environmental Testing Solutions. Inc.

Alkalinity (SM 2320 B) Matrix:* Water, RL = 1.0 mg CaC03/L Time initiated ~-'::==-.....;:::,.._::::::--,--1 Titrate samples to Time completed ""'""'"'

                                                                                                                                                        '--------1 pH= 4.5 S.U.

Titrant normality and multiplier determination: pHOI Normality Normality (N) ofH1S0 4 pH Factor or Multiplier Deionized Titrant checK *o* li'nd Total - (S ml Na1C03 x 0.05)/E = (N x 50000)1100 ml sample water reference standard ml ml ml =0.251E =Nx SOO

         -4.5S.U.             number                  number                           (E)   (acceptable range= O.u.uv -
  • Laborato control standard:

Reference standard True value Alkalinity (MV) 0

                                                                                                                                                      /o RS = MV I TV X 100 number                             (TV)                                        Total      Multiplier         (mg CaCOJI'L)                (acceptable range (mg CaCOJI'L)                                         litl                                                       =90 to 110%)

100 (1_1+ t-o.t} q~ q?>0 1o Alkalinity %RPD= Multiplier (mg CaCOJI'L) {(S- D) /[(S+D)/2)} x 100 (acceptable range= :1: 10%) Spike alkalinity (A) Total Multiplier (mg CaCOJIL) ml

                                                                                         .)   ta.l          \D.4                             11"0 Sample alkalinity (B)           Measured spike value (MV)                % R = MV I SV x 100 (mg CaCQJI'L)                      MV=A-B                               (acceptable range II samplel measurements:
                                                               ~9 (m2 CaCOJI'L)
                                                                                              '0'
                                                                                                                                    = 75 to 125%)
  • l Qll)lo I Sam_l!le number
  • ell os.()& ~~A ':-An SampleiD
                                                             ,,.sr 3 Sample volume 5D (ml)

Begin ml 1(1).1 End ml 11.3 Total ml a-'2- 1\?.J Multiplier lOA* Alkalinity (m2 CaCOJIL)

                                                                                                                                                          ~ t.~

I Jo n ~ \.01.. k'J' A~J'\ \Q\u~ IOJ l O~.'Z.\

                                                                     \

2. H.~

                                                                                            \14-.S
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                                                                                                                  -3.2.
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                                                                                            ~\, \      IJ4.l. 3.1
                                                                                                                   ~-~                                   lDC'J w

JO 1)~.~ 2'%. 2. d4.J.. l;;r.,s :S.:z. lot\ I I

          /OHO~.OI                                      /1         3                        B1.'5      ~q_        _3_._"-t                               -:J-\

J 0 110~.13 I i'fA 5Jttoa\ ~A ~.q 1~.'2, 4.~ ~~ I 10 r to~.N \ lliCTAA Reviewed by: 35.2 '31:)~ 4-S .,;I.... Date reviewed:

                                                                                                                                                          '14 I l l*U'1*.0            J lage of 71  I 05                                                                                                                SOP C6 - Exhibit C6.l, revision 09-0 l-09
       =er.,.£.,,.

J ._*. . r---'

                                            '                                                                                         Page       5
                                                                                                                                                             ~

Page -=--- of_,S"'---

       )   Environmental Testing Solutions, Inc.

Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Analyst Date analyzed I P,-&(... ll* OJ-I p Time initiated ,........_ Time completed 1--=-~=-----l Titrate samples to pH= 4.5 S.U. pH Factor or Multiplier

                                                                                                                                   = (N x 50000)1100 ml sample water            reference               standard                                                                                    =NxSOO
      = 4.5 S.U.           number                  number                               (acceptable range= 0.0180
  • 0.0220)

Laborato control standard: Reference standard True value Alkalinity (MV)  % RS = MV I TV x 100 number (TV) Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJIL) inl = 90 to 110"/o) 100 cvt 1~.4 q~ 95f0/o 0 Alkalinity /oRPD= Multiplier (mg CaCOJIL) {(S. D) /[(S+D)/2]} X 100 (acceptable range= :1: 10%)

                                                                                                        \0!      s D

j_ Spike alkalinity (A) Begin End Total Multiplier (mg CaCOJIL) ml ml ml 4.0 \~0 CJ.o a. \v.~ \qo Sample alkalinity (B) *Measured spike value (MV)  % R = MV I SV x 100 (mgCaCoYL) MV=A-B (acceptable range (me:CaCoYL) =75 to ll5%)

                                                         ~5                              105                                  l D'\:5°lo sammet measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOJIL) I \l ll 0~ .llo "fJ'AsH~ Af,~-r '5D 13-C 1::1.\, 4.\D lti) lC 9{p IO*.&b*l 0~ mi-\C.. uu \ 100 1-:J-.~ ~~.'+ S.<l. Ito 11*J.H **o J 2.. I ..23.4 act.'2.. 5.~ Lzc f 3 \ 35.1 s.g_

                                                                                                                                                 ~*

ll*C"Z.*IO ~tl.2 Jollo"i.~ !GoA,'\l,"'GJ Bea.tn ~ as.1 ~ S.-3 ~ 1- I tO

                                  /
                                       /
                           /                                                                      ./J.
                                                                                                  ~        "'

Reviewed by: Date reviewed: I i\-a-:l*&O SOP C6 - Exhibit C6 .1 , revision 09-0 l-09 72 of lOS

I :_*cy,;;!j J~ .

                                       ~                                                                                                 Page _ _

Page CfCJ. l of ,_ I _j Envfronmentol Tesdng SalulloM,Inc. Total Hardness (SM 2340 C) Analyst ,l-f/b;"-""""-=-----1 RL = 1.0 mg CaCOJI, Time initiated I {)9_~ Date analyzed (C)*'31.1V Time completed ~==fi~O=/~'}=====~ Normality (N) of EDTA pH Factor or Multiplier

                                                                                                           =0.2/E                      = (Nx SOOOO)/ SO ml sample (acceptable range= 0.0180 - 0.0220)                  =Nx 1000 c1.

Lba orato~ controI stantJard.:

  • Reference standard True value Sample Hardness (MV)  % RS = MV I TV x 100 number (TV) volume Begin Enct' Total Multiplier (mg CaCo,/L) (acceptable range (mg CaCo,/L) (_ml) ml ml *ml =90 to 110%)

IH~S(j~'l 40 50 ,,1 12.~ 2.D ~o. z., 1..{(.) j(J,ll>.l) y* Sample Hardn(!SS %RPD = Sample Sample ID . volume Begin Multiplier (mg CaC()JIL) {(S- D) /[(S+D)/2)} x 100 Matrix spike recov~ 1')1: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL)

                                           . (mg CaCOJIL)         (ml)      ml       ml         ml 11\i ~S~.:t::J                         40            60     l'f.O      J9.()      &../.o        !lo.~                   Sl Sample hardness (B)           Measured spike value (MV)                % R=MV /SVx 100 (mg CaCO.YL)                        MV=A-B                            (acceptable range (mg CaC()JIL).                       =75 to 125%)

L(Z,. 3t) QfJ:S I. samplel measurements: Sample volume Begin End Total Hardness Sample number SampleiD (ml) ml ml ml Multiplier {me: CaCO.vLl Blank TV=ND (should be = 0 mg CaCOVL) 61J 0.0 0.0 o,o ~~~ NO 10 ~:S_.10C 1lll~ J..j_'() 18.0 *a.~ ~.1- ~ 10 . .30

  • IO C\ l~*r a~.l 4.4 _1~

lo *3CHO B A~., _g , " -i.'S' ClJ IO*~*lO a 31.u a,., "i. S' Of I 10*~~- .0 8 ' Jll.l 40*~ l"f.S 0, I 10/Dl.la. (J I ~~ n ~.e~t. w1 , Q~ 1ov '/1/j t{._3 ZJ_ 11~- I% oI z. l 1'\.1 R.D t.( ,'i I /q8- d,IJ{j I_DJf.l_'2.L* IOJO!Q.(A I 3 ltl.o )Iff. '-1 s.'i ' Alfl_ * ,Ltt, I OIO 2.C. .n,_ T'"" v1 e &AJJJJ.. t ~ AY*1 ~~* 5 " ,I .A-/Z, Note: If >!Sml of titrant is used, sample must be diluted. Reviewed by: IL----I.ZI:;.,.l,.._ ___. Date reviewed I ffJ* ~l*J() SOP C7 - Exhibit C7 .I, revision 09-0 l-09 Page 73 of105

            !E. . ,J,.
            ~
            ~

l7. .. S ..,,...." Environmental Testing Solutions, Inc. Total Hardness (SM 2340 C) RL = 1.0 mg CaC03/L Analyst I ~fi)-:J Date analyzed ~=~J~b:."~=*=I~==~ Total ml ml Hardness (MV) o/oRS=My/ number Multiplier (mg CaC<>JIL) (acceptable range I =90toUO%) I Sample SampleiD Sample volume Begin Hardness (mg CaC<>JJL)

                                                                                                                                                       %RPD=

((S *D) /[(S+D)Ill} x 100 ml I Duplicate (B) I Matrix Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaC()JIL) I I Sample hardness (B) (mg CaC03iL) Measured spike value (MV) MV=A-B I sampjel measurements: Sample volume Begin End Total Hardness I Sam_IDe number TV~~ Blank SampleiD (should be- 0 mg CaCO:YL) (ml) ml ml ml Multiplier 11 (mg CaCOJI'L)

            /(}10"2§.     .en-          -rvA uno wtiJ.                        ,. 50             J.u.~ ~8-S" ~Q               iD.~                 40 IOit"l~       .04                                   I           3         I           ~6-~      3D:#"    ;..a.-                           t.f'1
                                                                                              ~

v--r

                                                                                                          \. ~

1-

                                                                                                                    ~           ------

Note: If >ISm! of titrant is used, sample must be diluted. Reviewed by: Date reviewed I /o*.3J*IO IPage 74 of105 SOP C7 - Exhibit C7 .1, revision 09-01-09

Page__.___ l l _I iETS

               -~ Envlroomontaf Testing Solutlonr,lnc.

Page _.!__of 5 l _I Analyst I~ Date analyzed ~

  • 10 I

I Titrant Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference ml ml ml =0.2/E = (N x 50000)/ SO ml sample number (acceptable range= 0.0180 - O.OllO) =Nx 1000 o.o q.~ q.~ o .o2.o tq 2c."' Hardness (MV)  % RS =MV I TV x 100 Multiplier (mg CaC03/L) 2-o. \i' 3

 ]                                                                                                                                          Hardliess Sample ID                                                              Multiplier            (mg CaC~)

-J J Spike hardness (A) number End Total Multiplier (mg CaCO]/L) J ml ml Sample hardness (B) Measured spike value (MV) %R=MV/SVxlOO (mg CaCOJIL) MV=A-B 31-amp1 e measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mK CaCO]/L) Blank TV=ND (should be = 0 mg CaCO:YL) 6D 1\'. .s \'P.5 O.Q 2S).'f;l ).Jp 1_:

                \\.oz..,c l\*C~>iD m\-\5 tho                                       1~.~
                                                                                          ~~~-0
                                                                                                                 ~3.o
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I C\3

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1\*D\*lO ~::f-~ ~I.I.P 4.~ ~6'1] u- '"' n ~ '2. tf'J!.

                                            ~rd.lo.. tr'Q ("\         \
                                                                                           ?11.~- M.l                       ~-s                                    '5Z.

2 52.. lo II b'-t

  • V1 \
                                                         \            5
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I~"*" c:il.'5 u /~lloi..Q"l..... ~~-4.P ~X.1'

                                                                                                                             ~'                                   l{3
               /0 IIOLO            l SoiU.l.U' Pihi                 \                     !~$.1-               l4._k         l.'l                                 3'\

Ib It O'lt. 'Z.b z. .40.1n ~~-u. 1.~ 31 L 10 u os. ol./ I

                                                          \        3                       4~.4 1~.~                          ).q                  __._           3G\

l: Note: If >ISm! of titrant is used, sample must be diluted. Reviewed by: '-1.....:.ll'o+=--..:._:_ _ _ . . . .~ Date reviewed I ll*O l* ,u Page 75 of lOS SOPC7-ExhibitC7.1,revision09-0l-09

                ~
                  . ETS
                ****        ,,,,     -~*-'     1,:.-\'

Envfronment*l Testing Salutfon~rlnc. Page .ri Page -'z..::::....__ of...::S~*-- r= Total Hardness (SM 2340 C) RL = 1.0 mg CaC03/L Analyst Date analyzed l1-1)0

                                                   ~
                                                     ,~.o:;..c_i-_*_i_0_--1 Time initiated Time completed               "'""'"4 Titrant normality and multiplier determination:

standard t'he.. k Begin End Total Normality (N) of EDTA pH Factor or Multiplier (Nx 50000)150 ml sample reference ml Ull =O.l/E number number (E) (acceptable range *_u.v*_o~

  • u.  !' =Nx 1000 True value Sample Hardness (MV) o/o RS = MV I TV x 100 number (TV) volume Begin End Multiplier (mg CaCQ,IL)

Miatrix ¥1ikerecover.r: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End TotaJ Multiplier (mg CaCOJil,) (mg CaCQJIL) (ml) ml ml ml J:"\'oJS~ <b11- 40 5b t<g f).<; 3.1-Sample hardness (B)

                                                                                                                     ~.~

Measured spike value (MV) o/o R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mg_CaCQ,IL) =75 to 125%) 3~ 6'\ ~'\~.f;~. ) s~amp*el measurements: Sample volume Begin End Total Hardness Sample number SampleiD _(ml) ml ml ml Multiplier (me: CaCQ,/L) Al,.nlr TV=.-..~ (should.be = 0 me: CaCO]/L) I 0 H O'l..O l CJ:..iJ IL iJrt \ ~s 5.5'" q~ #-f.3 £i) 2.D.'-IJ ~i&St &1(0 JU II b'-1.01 t 2- I ~-~ l?>.'i ~.i I I

                                                                                                                                                                  *~0 I

10 II Of..((,!, Jb 11 o \.o'i -.v A ' A:\i=tn\

                                                                     ..2
                                                                                         }0 l3ll      tt4 11-.4 IJs.<l.

3.5

                                                                                                                            'a.li-      ~                           *~
                                                                                                                                                                    'e~c-eM'S I_D IJ{B.l~                                   I
                                                                \
                                                                              'z                        ..25$     ~-~       co .t;        II                       ta'ao
  • 0':lt.

101lOS. O'l .3 &+~ ~-'+- -:J.) 'l3o-'=t~\

                   /OitOI*OS                 hJ A PAf \~\                   l            a6             .&fl.'+ ~-I         ~.":J      ~')                         1\0 - l\1

_l_O Ho~.1.'1 \ z. \ 1--H.\ ~.(( ~~ I \20 *L\~ I .) I H\p.q 1~!1 I lbaJOS.ro 8.0 _~..-

                                                                                                                                                                   \ '2o - \'l.~

t, Note: If >15ml oftitrant is used, sample must be diluted. Rev1ewed by: l..____l_~-~--.....1 Date reViewed I \ \.\)1- *10 'i Page 76 of 105 SOP C7 -Exhibit C7.1, revision 09-01-09

Page _,3::o..__

             !ETS
             ~    EnvlronmontaiTulfng Solutions. Inc.

Page 3 of_,5=---- I Analyst I ~St.. Date analyzed 1-l.Lil~:D:.:1:...._*l-0---1 I Titrant normality_and mu fP~ er 'etermination: Ti ....... nv* ouMuy l:tun:ft. u;: m I:IUU Normality (N) of EDTA pH Factor or Multiplier I reference number standard number 1 ml ml (E)

                                                                                                                =u**,_

(acceptable range 0.0180. 0.0220)

                                                                                                                                             - (Nx 50000)150 ml sample 1\T.., 1000.

I control Reference standard True value Sample Hardness (MV)  % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC<>JJL) I z.c."' 3<1 I End Total Multiplier Hardness (mg CaC{M..) ml ml I I Miatrix sp1lke reco-very: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaC031L) I .:t'NSS <62-:t (mr CaC<>JJL)

                                                       ~0 (ml) lb ml 0*~

ml (c..2. ml 3.0 f)) ').J) ."' ;3lo I Sample hardness (B) (mg CaCOliL) Measured spike value (MV) MV=A-B

                                                                                                                                  % R = MV I SV x 100 (acceptable range
                                                                                                                                      =75 to 125%)

(mr; CaC03fL) I sampie l measurements:

                                                              \'Z..o                                 1qo                               qst~fo Sample volume        Begin      End        Total                            Hardness I             Sample number TV        ND                 Blank SampleiD (should be- 0 mg CaCO:VL)

(ml) ml ml ml Multiplier

                                                                                                                                  -" ~eSI.-

(mr; CaC03fl,) JJ:ll' 0~. 2.. 1JA Pfh:"oo,, tJTM B.~ (t.2. G.? 3.1 ~ 1.D.\a ' ao . t'Z.S _l_O_IL 0~.1.<<; 00'2. AP I'D 'l.; to.s Ll.. (S) 2. \ ~"' 0 I I b1a. '1.~ 'NJ Rf as; 10-'5 )-=\,!:) 21.0 (() 1'2.-o ... '~"~ I I to IIO\.a1- ~{A SG.n LD~ \ 5'D 13.':5 13_.\) 8.'5 1-2.

                /Jj_}l ()'!!,. 2.\                       I             z                        \t.O       ~'O,Y.       -~-*                            -:}()

I .1n 1 1 o~.Cf1 Jo 1101.~ frJA SQn ,~'.)\

                                                          \          ..3                         ~o.L.l-   ~. ~.'t o?~_q J':l-l
                                                                                                                        ?..c; 3.;
                                                                                                                                                         ?2.

lt~ I 1Il I \ m:t.Ot. I ~ _, ~~-2 ~0-~ 3.'+ ":fo I _10 uos.. 0 f Note: If >lSml of titrant is used, sample must be diluted. l Reviewed by: I 30.\o ~.0 3.Y. 1AL~ Date reviewed

                                                                                                                                                       ":fO I \\ .o 1* ~

Page 77 of 105 SOP C7- Exhibit C7.1, revision 09-01-09

I __.tp.m-" *--

             .*.ETS Page _J.f.

I *

  • Page J.f of-=5'----

I *~ EnvlranmentaiTestlng Solutions, Inc. I Analyst ~&zc..,wg,'-------1 Date analyzed 1\ *Ct *10 I Titrant normality and multiplier determination: T3 -" check Begin End Total Normality (N) of EDTA pH Factor or Multiplier I reference number standard number 1111 ~I (E) (acceptame

                                                                                                             =O.l/E
                                                                                                                         -1Ul110\
                                                                                                                                       = (N x 50000)150 ml sample
                                                                                                                                                 = N..x 1000 I                                                                                                                        Hardness (MV)         % RS =MV I TV x 100 Multiplier     (mg CaCO]/L)            (acceptable range I                                                                                                                                                    =90 tollO%)

I Hardness (mg CaCQ31L) {(S- D) /[(S+D)/l)} 1 100 I s I Matrix smike recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO]/L) I  :!'N~~'l (mg CaCQ31L)

                                                        "lO (ml)

SJ ml ml Cfl.z, l*tb ml 5.~ 2.0.\p 1\0 I Sample hardness (B) (mg CaCO,!L) Measured spike value (MV) MV=A-B

                                                                                                                            % R = MV I SV x 100 (acceptable range
                                                                                                                                =75 to 125%)

(mg CaCO]/L) I s.amp*l e measurements: 1-0 JfD toct>lo Sample volume Begin End Total Hardness I Samole number TV-ND Blank SampleiD -(ml) ml ml ml Multiplier L (mR CaC03/L) I Ill\\ en,. 'Z.\ 11 A57!\(\ luh.>u z so l~.c; at&:o 3 .'5 2..\).\9 "12. 1o no~. 01 I ~ 0.0 3.\1 6.\Q i'+ IO lltll.~ 1J A- 5/\.f\ l tS'ii)J 1 ~.\p q,J 3.'5 ":1'2.. I /D II ()!J.1.'1- I

                                                              \
2. 1-:t. \ lO.S .3.4- -=to
                                                                        ...a                                      a.cg lo IJ OS .oR                                                                                 ILf.~
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JoJJ~.I'P bJTftP 03.\o 3'-l-':t I ~** Revtewed by: I~..-_-IYn,J.Aoo.M--...J Date revtewed I L\.(13 .jQ r Note: If >15ml of titrant is used, sample must be diluted. Page 78 of 105 SOP C7 -Exhibit C7.1, revision 09-01-09

Page _,5::...___ iETS

                ..:) Environmental Testing Solutions, Inc.

Page o of--=5~- Total Hardness (SM 2340 C) Analyst /J--L.:aillos.C:c.,::;.__ _~ Date analyzed u. t>l* 10 RL = 1.0 mg CaC03/L Time initiated Time completed .__ _ It->~""'1::::~----t Titrant ..:~yand .... .. __ J, .:. ..:, ,.

                  ~~~-

Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier I 'w* ,-;s:-

                                  ~

standard number ml ml ml (E)

                                                                                                                                      =0.2/E
range= 0.0180
  • 0.0220)
                                                                                                                                                                      = (N x 50000)1 SO ml sample
                                                                                                                                                                                 =Nx 1000 I            1.* -..                  -~               J. J.
                                                   ~~:.\

Reference standard Sample Hardness (MV)  % RS=MV /TVx 100 number volume Begin End Total Multiplier (mg CaC<>J/L) (acceptable range I 40 (ml) 50 rr11 ml ltd =90 to 110%)

               ... ....                                 -**                         ~

I *san vie

                                                                                             ~

Sample Hardness %RPD= Sample SampleiD volume End Total Multiplier (mg CaC{)JI'L) {(S

  • D) /((S+D)/21} x 100 number (ml) ml ml

-I

                                                                                                                                                                      ~

s

                                                                                                             ~

D

                                                                                                                  ~

I Duplicate (B)

 *I            Matrix spike Reference standard number Spike value (SV)

(m2 l"at'O.tr \ Sample volume (nd) Begin ml End ml Total ml

                                                                                                                              "    Moa~r Spike hardness (A)

(mg CaC{}JI'L) i Sample hardness (B) (mg CaC<>JIL) Measured spike value (MV) MV=A-B (~gCaCoYLl

                                                                                                                                                          %R *~/SV 100
                                                                                                                                                               *7Stol~
*~              amp* e measurements:

Sample number TV LUI _Blank

                                                           *SampleiD Sample volume
                                                                                                    . (ml)

Begin ml End ml Total ml Multiplier Hardness Cm2 CaCOJILl (should be= 0 mg CaCOVL)

  ~              lb-~* J(') ~

ll*0\*10 ffiHs I lh) .l 2-

                                                                                                  '5D I

3't1 4';.~

                                                                                                                                 ~.i
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                                                                                                                                       -....                       -~ ~*
  -           Note: ff >ISm! of titrant is used, sample must be diluted.                       Revtewed by:                   l~f:'A.

L-...___;~:...;...::=--..:.......1 Date revrewed I llw03- 1-0

  ~Page 79 of 105                                                                                                                                     SOP C7- Exhibit C7.1, revision 09-01-09

Sequoyah Nuclear Plant Biomonitoring November 2-9, 2010 Appendix D Reference Toxicant Test and Control Chart Page 80 of 105

            **                                                                                              Pimephales promelas
            ~

Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc.

            ~     Environmental Testing Solutions, Inc.

USEPA Control Limits (+/- 2 Standard Deviations) 1.0 0.8 0.6 0.4 1.2

       ;::--                    USEPA Wa~!"ing and C~~~q!Limits..(75~~.and.9.0t~.f.~t9.f!r!J#f!..9.YsJ.. ......

u -**-*- .. -**- *-**-**- . 1.0 -**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**

       ~
       ~

u

       -   Ill N

0.8 0.6

       """"~                   -**-**- .-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**- *-**- *- *- *-**
        =
      "CC I

r--- 0.4 ..................................................................................................................................................... 1.2 Laboratory Warning and Control Limits (lOth and 25th Percentile CVs) 1.0 0.8 0.6 ****************** ********************************************************************************************************************************** 0.4 Test date 7-day IC25 = 25% inhibition concentration. An estimation ofthe concentration of potassium chloride that would cause a 25% reduction in Pimepha/es growth for the test population.

   - - . Central Tendency (mean IC 25)

_ .. _ .. _ Warning Limits (mean IC25 +/- SA 10 or SA75)

   .............. Control Limits (mean IC25 +/- SA 25 , SA.90 , or 2 Standard Deviations)

Page 81 of 105

oJ -~*-- ** ....< *--=...!lL~l<<o.:!~-*~*~o*..>.J>..--"'""~'"-..........._ .** _ * ..::.=n=..<::~""-'"<.t>.="'"""'- **=**=-+-+...~~-~ ~ ~ ~ ~ ~ ETS ~ {f) Pimephales promelas Chronic Reference Toxicant Control Chart

     ~      Environmental Testing Solutions, Inc.

State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-cbly IC,. CT s Control Limits s ..... Waning Limits s...,. Control Limits s.._.,. Waning Limits s ..... Control Limits cv (WLKCI) (g/LKCI) CT-ZS CT+ZS CT-s.._., CT+Suo CT-S.._,. CT+S.._,. cr-s.._.,. CT+S.._75 cr-s.._,. CT+S.._,. I 04-06-10 0.62 2 04-06-10 0.76 0.69 0.10 0.50 0.89 0.08 0.61 0.78 0.15 0.55 0.84 0.26 0.43 0.96 0.31 0.38 1.01 0.14 3 05-04-10 0.70 0.69 0.07 0.56 0.83 0.08 0.61 0.78 0.15 0.55 0.84 0.26 0.43 0.96 0.31 0.38 1.01 0.10 4 05-11-10 0.72 0.70 0.06 0.58 0.82 0.08 0.62 0.79 0.15 0.55 0.85 0.27 0.44 0.97 0.32 0.39 1.02 0.08 5 05-18-10 0.77 0.72 0.06 0.60 0.84 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.08 6 06-08-10 0.66 0.71 0.06 0.59 0.82 0.08 0.62 0.79 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.08 7 06-15-10 0.72 0.71 0.05 0.60 0.82 0.09 0.62 0.79 0.15 .0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.08 8 06-22-10 0.77 0.72 0.05 0.61 0.83 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.08 9 07-13-10 0.75 0.72 0.05 0.62 0.82 0.09 0.63 0.81 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 O.D7 10 08-03-10 0.76 0.72 0.05 0.62 0.83 0.09 0.64 0.81 0.15 0.57 0.88 028 0.45 1.00 0.33 0.40 1.05 0.07 II 08-17-10 0.79 0.73 0.05 0.63 0.83 0.09 0.64 0.82 0.15 0.58 0.88 0.28 0.45 1.01 0.33 0.40 1.06 0.07 12 09-14-10 0.75 0.73 0.05 0.63 0.83 0.09 0.64 0.82 0.15 0.58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.07 13 09-21-10 0.73 0.73 0.05 0.64 0.83 0.09 0.64 0.82 0.15 0.58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.07 14 09-28-10 0.65 0.73 0.05 0.62 0.83 0.09 0.64 0.81 0.15 0.57 0.88 0.28 0.45 1.00 0.33 0.40 1.05 0.07 IS 09-29-10 0.68 0.72 0.05 0.62 0.82 0.09 0.64 0.81 0.15 0.57 0.87 0.27 0.45 1.00 0.33 0.40 1.05 0.07 10-05-10 0.70 0.72 0.05 0.62 0.82 0.09 0.63 0.81 0.15 0.57 0.87 0.27 0.45 1.00 0.32 0.40 LOS O.o7 16 0.72 0.72 0.05 0.63 0.82 0.09 0.63 0.81 0.15 0.57 0.87 0.27 0.45 1.00 0.32 0.40 1.05 0.07 17 10-06-10 10-12-10 0.70 0.72 0.05 0.63 0.81 0.09 0.63 0.81 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.06 18 10-26-10 0.68 0.72 0.05 0.62 0.81 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.06 19 11-02-10 0.70 0.72 0.0~ 0.63 0.81 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.06 20 Note: 7-cllC,.- 7-day 25% inlnbition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimepbales growth for the test population. CT = Cenlral teodency (meao IC,,). S = Standard deviation of the IC" values. Laboratory Control and Wal"llln& Limits Laboratory control and warning limits were established using the standard deviation of the ICzs values corresponding to the lOth and 25th pereentile CVs. These ranges are more stringent than the control and warning limits reconunended by USEPA for the test method and endpoint. s.._ .. - Standard deviation corresponding to the 10.. percentile cv. (SA.IO = 0.12) S.._,. = Standard deviation corresponding to the 25,. percentile CV. (SA.lS = 0.21) USEPA Controlaud Wamiag Limits S.._75 = Standard deviation corresponding to the 75'" percentile CV. ( SA.7> = 0.38) S.._..= Standard deviation corresponding to the 90'" percentile CV. (SA90 =0.45) CV = Coefficient of variation of the IC,. values. USEPA. 2000. U~g and Acalunling for Me1hod Variability in Whole Eflluent Toxicity Appticatioos Under the Naliooal P'ollutant Discbatge Eliminalioo Program EPA-833-R-00-003. US l!mironmenla1 Protection ApY:y, Cincimali, 011. ppkdcr_110210 OI"JI'llllsms obtained.from AquaJOX. Inc.

Precision of Endpoint Measurements Pimephales promelas Chronic Reference Toxicant Data Control Control Mean Test number Test date Survival Growth CT cv CT MSD PMSD CT for Control Growth for Control (%) (mgllarvae) (%) (%) for PMSD (%) (mgllarvae) Growth cv (%) I 04-06-10 100 1.101 4.9 0.10 8.9 2 04-06-10 100 1.010 1.055 7.9 6.4 0.14 14.3 11.6 3 05-04-10 97.5 0.871 0.994 9.7 7.5 0.13 15.0 12.7 4 05-11-10 100 0.901 0.971 1.8 6.1 O.o7 7.7 l1.5 5 05-18-10 100 0.754 0.927 5.9 6.0 0.11 15.2 12.2 6 06-08-10 100 0.808 0.907 14.4 7.4 0.14 17.5 13.1 7 06-15-10 100 0.760 0.886 9.4 7.7 0.09 12.5 13.0 8 06-22-10 100 0.882 0.886 10.1 8.0 0.09 10.7 12.7 9 07-13-10 97.5 0.933 0.891 3.0 7.5 0.08 9.0 12.3 10 08-03-10 100 0.783 0.880 3.6 7.1 0.04 5.4 11.6 11 08-17-10 100 0.952 0.887 4.2 6.8 0.16 16.5 12.1 12 09-14-10 97.5 0.813 0.881 5.9 6.7 0.11 13.9 12.2 13 09-21-10 100 0.891 0.881 7.5 6.8 0.07 7.5 ll.8 14 09-28-10 97.5 0.991 0.889 9.5 7.0 0.11 10.9 ll.8 15 09-29-10 100 0.893 0.889 8.5 7.1 0.11 12.1 11.8 16 10-05-10 100 0.912 0.891 8.1 7.2 0.09 9.5 11.7 17 10-06-10 100 0.810 0.886 2.3 6.9 0.06 7.4 11.4 18 10-12-10 100 0.799 0.881 4.1 6.7 0.10 12.0 11.4 19 10-26-10 100 0.779 0.876 10.0 6.9 0.13 16.8 11.7 20 11-02-10 100 0.727 0.868 10.9 7.1 O.ll 14.5 11.9 Note: CV = Coefficient of variation for control growth. Lower CV bound detennined by USEPA (lOth percentile)= 3.5%. Upper CV bound determined by USEPA (90th percentile)= 20% MSD = Minimum Significant Difference PMSD Percent Minimum Significant Difference 2 PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effiuent toxicity test. Lower PMSD bound determined by USEPA (I Om percentile) = 12%. Upper PMSD bound detennined by USEPA (90th percentile)= 30%. CT = Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effiuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-tenn Chronic and Acute Whole Effiuent Toxicity Test Methods, Volumes I and 2 Appendix. EPA-821-B-Ol-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtainedfrom Aquatox, Inc. ppkclcr_II0210 Page 83 of 105

             ***                                                                                         Pimephales promelas 8     =-'-'=~.

Chronic Reference Toxicant Control Chart PrecisiQn of Endpoint Measurements

             ~     Environmental Testing Solutions, Inc.

Organism Source: Aquatox, Inc.

         .=
         . . t=-~   1.00
          .=-= 0.75
           ~

c.?"; ********************* ***********************************

          ..=...= 0.50
         --~
          =-

u= ~

               -   0.25 USEPA Acceptance Criteria(> 0.25 mg per surviving larvae) 0~           30
        .s=

..., Kentucky Acceptance Limit (< 30.0%)

               ;9
                *~

1C = 20

       *c = ~""
       ....    ~                                                                                                ................................................................
          ==
       ....5u u
           . =

10 IS cS

          ~

0 u= 30 USEPA Upper PMSD Bound (90th percentile < 30.0%) 20 ........ .... ................................................... .... 10

  • Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant Central Tendency (mean Control Growth, CV, or PMSD)

Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) Page 84 of 105

Page 1 of5 iETS

        ~ EnvtrvnmentiiTestlngSolutJDnSrlnc.

Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas PpKClCR Test Number: ~ Dilution preparation information: Comments: KCl Stock INSS number: I H~&& l I Stock preparation: Dilution prep (mgiL) 450 SOg KCIIL: Dissolve SO g KCl in 1-L Milli-Q water. 600 750 900 1050 I Stock volume (mL) DUuent volume (mL) Total volume (mL) 9 991 1000 12 988 1000 15 985 1000 18 982 1000 , 21 979 1000 I Test organism information: Organism age: 1'\ ,'\'2. ..tov6 0\.1:) Test in,{orination: Randomizin_g template: (t~~ Date and times organisms Incubator number and I \\* 0\'"&0 \\oC)() were born between: shelf location: .3-.= Organism source: A"TOY- 6.-m.\-\ ~,.., a\-0\-.o Artemia CHM number: _c.t\ lo\ Sl l Drying information for weight I oc determination: Transfer vessel information: pH=

                                                       ,.,'l     S.U. Temperature=
                                                                                      "'1~.1.

Date I Time in oven: Initial oven temperature: l\-o~*lO

                                                                                                                                   ~~*t_.

IH~ I Average transfer volume:

o. &e,"("/ -4.

Date I Time out of oven: Final oven temperature: l\*&O*tt)

                                                                                                                                \oO'C:.

IUS Total drying time: "1.'-\-~o\J.t.C:, I Daily feeding and renewaJ information: Day Date Morning reeding Afternoon reeding MHSW I batch used 0 I 2 I 3 4 I I I I IPage 85 of 105 SOP AT21 - Exhibit AT21.1, revision 04-0 1-09

I I

          *ers
          ~ .,,*.,: *.,.'   1_,_,  ,,_.,,.:,_
          ~ Envtnmm....I'!Utlng Soludon~ In<.

Page 2 of5 I Specie,s: Pimeohales oromelas PpKCICR Test Number: ~ I Day l,;ORtrOI Survival and "- 4SJ) m1 *KCIIL

                                                                                                                       .::~Data
                                                                                                                                              ~QQ ml!     KCIIL A         B        c         D               F                                                              L I                                     0 lo       IO         IQ     IO       to E
                                                                                                        /0 G

IO H IO /Q I to J K IO IO 1 to I 2 lb to 10 10 IC IC /Q It;, 10 IQ

                                                                                                       /C)        10 10
                                                                                                                            /0 lO        ro IO     /Q 10 10 IO I C) 10 I                                     3 4
                                                            /0      10        10       I()       I() ' 10           10        /0
                                                                                                                                               . t\~ '\ ,r.\_
                                                                                                                                                                       /0
                                                           /0       10         10      IO        ID      10       I(:)     IQ            '\       '\         C\        IO I                                     s to                (C)       (0                                                                  q            ro I

6 10 10 ll) ll) 10 IC I() 10 IC I() {Q JQ IO

                                                                                                                                      <\
                                                                                                                                       'i      "'\         '\           /Q 7

to 10 10 ~cr 10 10 /C rob- '\ '1 i *crt 10 I A= Pan weig~~~!'la. \l~ Traycolor -"*

                                                         \'1~ ,~.'tS'       t'--~ \"\.'~\ ~~~       H.t\      \~1;,    \~*~ ~~\)\\ \~}.\~ lfl.\1\ \'-l.ut
                ~~;st\r.~.... IO I                 B =Pan + Larvae weight (mg)

Analyst: M lol-~ lt.o tt. t-t.V{ ~l.'\!1 £0.~ t.tt.IC. t:f...(.':\ u.~ 'f,.~.'ll 'l,l .<< 1-z-1 .t'\) t4.il," l'lA .'H. Date: \\ .\O lO I ,...'- ...,, ,.1.'-\ C = Larvae weight (mg)

                 =B-A
                                                         ,.OC\    ,.... ~   &.o\. c..1."!> &'-~' &*"\'-         ,.,~      ~\t        ,. t;.\

I ~~ight per inidal number

                                                                      ...        o'-
                                                                                                                                                               "        A._~

C)'~"" ()*"~'

                                                                                                         ~'~._.

oflarvae {mg)

                 = C I Initial number of larvae A>o..   ~
                                                                               ~
                                                                                      ..."'c.,  ~\      ~        ~'->                            ~          \o'\

()' o* 0' o* ()' \)' ()* ,I b' tY ()' Average Percent weight per initial number of reduction from control {%) 0."'\ 'L, o. tc:,o -11...1\'7. 0-11.'5 0 .~?. I larvae (mg) Coroment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg =unusually large, d&r = decanted and returned, w = wounded I Calculations and data reviewed: J{_ Comments: I I I

.:Page 86 of 105 SOP AT2l - Exhibit AT2l.l, revision 04-0 l-09

I Page 3 of5 I iETS

         ~ Envlronmentll'lUtlngSolutlons.lnc.

I Species: Pimeohales oromelas PpKClCR Test Number: l'Z.O I Day Survival and Growth Data 900 mt: KCIIL 750ml KCIIL lOSOm KCIIL u w I 0

                                                       /0 M
                                                              /0 N

10 0 lO p IO Q 10 R s IO T

                                                                                                                                 /D /0 v

IO (Q X ID 1ilj I 1 IO ca u( t:t*l\ q*c( dul &M 411.( lo a e.~. t 14 16.( 2

                                                        ~~      '\ '\                  a'.l      '-'lA      ,   ~   fo--w. cflJJ.           ~..~      5'4         lolA    ~

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                                                                                                                                  '"\        '2.        \                  -e-I               A= Pan weight(~

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                                                                         \1..'\\ \~-~        \1,..C\~ \?*13                  \1..*"~ \?*~\ \'\.\;               ~9o~ \~:t\

I Analyst: Date: '\ \.. tn.. \ 0 B- Pan + Larvae weight

                                                     \?rl\
                                                             "*           11. '6?>   \q.~
                                                                                                                  "*""                   l.l).o1 1

1~.1., 1~.>6-t II;,~ l~.r..~ \~.1; (mg) \~.~t Analyst: M H::k ll..ltf lb.1o 11.d Date: 1-\. to. lO I C =Larvae weight (mg)

               =B-A                                  s.os Sf\'-          ~.'\1 $.()'- -a..~              11>-"1 ,..1.1,.      '!t,l.l    '*'-'- \.0'\          O.lo'\    \.i:t I              Weight per Initial nnmber
                                                                                                                                 ..,J.

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               = C I Initial number of larvae o*
                                                       ~

o'- t;flt.."' o* lb*~"' ~

                                                                                     "        o*
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                                                                                                             ~ "3-,'V It)

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0~ I Average weight per Initial Percent reduction from control o.5l.<<o 'l..1*1 7. Q.3'-t\ S'!. *'2.7. O.l~"Z. rL'\1o number of (%) I larvae (mR) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. I Calculations and data reviewed: ~ Comments: I I IPage 87 of 105 SOP AT2l -Exhibit AT2l.l, revision 04-01-09

                                                                                                                                                                                   - *"~

_J .-.'-'~-"----'-'~=-=.-.==:.a.:....=..>=-~'-=-="'.>.~= -- ..... - ~ ~ Qo Qo ~ ~

     **                                                                                                 Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Metbod 1000.0 r:fY Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
     ~

Testaomber: PpKCICR#IllO Environmental Testing Solutions, Inc. T..t dates: November 02-09,2010 I ' "\1~ c........... (,..tL KO) A Iollillo-or 10 J'IMI*amiM:rr* 10 A*Puwtipl (IBI) 13.83 B*fu+Lanot wdPI(IIJI) 20.92 LarYoewdpt(IIJI) Wdgld I S.nlylac

                                                                                                                *A-B 7.09 McuwdPU oolllbcrorlorne(llll) - * * - b e r o r 0.709 Cotllideat or varllllioll
                                                                                                                                                                       <M- .......... _ . . .

_.....,...._)(%) Wdpt/laidllloolllllu orW.oe(llll) 0.709 MeuiRI'ViYal MouweiPUioldll (%) ....ber~Jary-(IIIII

                                                                                                                                                                                                                                                        '.A
                                                                                                                                                                                                                                                         ~or vll'ialloo(%)
                                                                                                                                                                                                                                                                           -*--coatrol(%)

B 10 10 13.75 21.44 7.69 0.769 0.769 Cootrol 0.727 10.9 100.0 0.727 10.9 Not applicable c 10 10 13.66 21.72 8.06 0.806 0.806 D 10 10 14.31 20.56 6.25 0.625 0.625 E 10 10 13.75 22.16 8.41 0.841 0.841 F 10 10 14.21 22.67 8.46 0.846 0.846 450 0.850 6.0 100.0 0.850 6.0 -16.9 G 10 10 13.58 21.53 7.95 0.795 0.795 H 10 10 14.53 23.71 9.18 0.918 0.918 1 10 9 14.04 21.55 7.51 0.834 0.751 J 10 9 13.44 21.10 7.66 0.851 0.766 600 0.805 5.5 90.0 0.725 9.9 0.4 K 10 8 13.69 19.86 6.17 0.771 0.617 L 10 10 14.07 21.71 7.64 0.764 0.764 M 10 7 13.59 18.64 5.05 0.721 0.505 N 10 8 13.77 19.73 5.96 0.745 0.596 750 4.97 0.828 0.154 6.7 0.497 70.0 D.5l6 8.9 27.7 0 10 6 12.91 17.88 p 10 7 14.40 19.46 5.06 0.723 0.506 Q 10 3 12.94 16.19 3.25 1.083 0.325 I R 10 4 1323 16.70 3.47 0.868 0.347 900 0.919 13.0 37.5 D.341 6.3 53.2 s 10 4 13.79 17.01 322 0.805 0.322 T 10 4 12.64 16.32 3.68 0.920 0.368 v 10 2 13.41 15.07 1.66 0.830 0.166 1050 v 10 I 14.13 15.17 1.04 1.040 0.875 17.2 0.104 15.0 0.132 0.0 81.9 w 10 I 14.82 15.51 0.69 0.690 0.069 X 10 - 2 13.71 15.59 1.88 0.940 0.188 - -*- Duaett'o MSD value: 0.1053 MSD= Minimum Significant Difference PMSD: 14.5 PMSD= Percent Minimum Significant Difference PMSD is a measure of test precisioo. The PMSD is the minimum percent differeru:e between the control and treabnent that can be declared statistically sisnificaot in a whole effiuentwxicity test. LowerPMSD bmmd detennined by USEPA (lOth percentile) = 12"/o. Upper PMSD boWld detennined by USEPA (90th percentile)= 30%. Lower and upper PMSD hoWlds were detennined from the I Otb and 90th percentile, respectively, of PMSD data from EPA's WET lnterlabonllmy Variability Study (USEPA. 200la; USEPA. 2001 b). USEP A 2001a, 200 I b. Final Report: lnterlabonllmy Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

~ ~ Qc 'C ~ ~ iETS

                             ; ) Environmental 'JesUng Solutions, Inc.

Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 11/212010 TestiD: PpKQCR SampleiD: REF-RefToxicant End Date: 11/912010 LabiD: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-ml!!!: I 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 600 0.9000 0.9000 0.8000 1.0000 750 0.7000 0.8000 0.6000 0.7000 900 0.3000 0.4000 0.4000 0.4000 1050 0.2000 0.1000 0.1000 0.2000 Transform: Arcsin Square Root Rank  !-Tailed Number Total Conc-ml!i!: Mean N-Mean Mean Min Max CV"Io N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 600 0.9000 0.9000 1.2543 1.1071 1.4120 9.935 4 12.00 10.00 4 40

                                     *1so         0.1000        0.1000        o.9939       o.8861        1.1011        9.086        4              10.00        to.oo                     12         40 0 900        0.3750        0.3750        0.6584       0.5796        0.6847        7.979        4              10.00        10.00                     25         40
                                    *1050         0.1500        0.1500        0.3927       0.3218        0.4636       20.862        4              10.00        10.00                     34         40 Auxiliary Tests                                                                                     Statistic                 Critical                 Skew       Ktut Sbapiro-Wilk's Test indicates normal distribution (p > 0.01)                                      0.91023314                   0.884                0.08328903 1.18006418 Equality of variance cannot be confirmed Hypothesis Test (1-tail, 0.051                NOEC          WEC           ChV           TU Steel's Msny-One Rank Test                      600          750       670.820393 Treatments vs D-Control Maximum Likelihood-Probit Parameter          Value          SE       95% Fiducial Umits                        Control        Cbi-Sq       Critical     P-wlue         Mu        Sigma       Iter Slope         9.94181564 1.30210515 7.3896895 12.4939418                                0        0.56435054 7.81472778          0.9     2.92077764 0.10058525      3 Intercept       -24.037833 3.79565674 -31.47732 -16.598346 TSCR Point             Probits        rna!!:    95% Fiducial Umits ECOI                   2.674  486.162395    402.073611 546.054153 EC05                   3.355  569.284857    495.375707 621.405179 ECIO                   3.718  619.259192    552.924847 666.630381 ECI5                   3.964  655.432146    594.893597 699.682767 EC20                   4.158  685.682341    629.945281 727.767856 EC25                   4.326  712.744582    661.059933 753.440058 EC40                   4.747  785.768588    742.116145 827.019952 EC50                   5.000  833.254457    791.085439 879.682466 EC60                   5.253  883.610019    839.281045 940.16349 EC75                   5.674    974.13997   918.466658 1058.63369 EC80                   5.842  1012.58695    950.174438 1111.73075 EC85                  6.036  1059.32095    987.708178 1177.95731 EC90                  6.282  1121.19929    1036.13459 1268.03499 EC95                  6.645  1219.62311    1111.01186 1416.02316 EC99                  7.326  1428.15045    1263.76751 1745.38103 Organisms obtainedfrom AquaJox, Inc.                                                                                                                                                                ppkclcr_II0210
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~ 1.) Environmental Testing Solutions. Inc. Larval Fish Growth' and Survival Test-7 Da;r Growth Start Date: ll/2/2010 TestiD: PpKCICR SampleiD: REF-Ref Toxicant End Date: 11!9nOIO LabiD: ETs-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-m~ I 2 3 4 D-Control 0.7090 0.7690 0.8060 0.6250 450 0.8410 0.8460 0.7950 0.9180 600 0.7510 0.7660 0.6170 0.7640 750 0.5050 0.5960 0.4970 0.5060 900 0.3250 0.3470 0.3220 0.3680 1050 0.1660 0.1040 0.0690 0.1880 Transfonn: Untransfonned 1-Tailed Isotonic Conc-m~ Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7273 1.0000 0.7273 0.6250 0.8060 10.866 4 0.7886 1.0000 450 0.8500 1.1688 0.8500 0.7950 0.9180 5.978 4 -2.541 2.180 0.1053 0.7886 1.0000 600 0.7245 0.9962 0.7245 0.6170 0.7660 9.934 4 0.057 2.180 0.1053 0.7245 0.9187 750 0.5260 0.7233 0.5260 0.4970 0.5960 8.905 4 0.5260 0.6670 900 0.3405 0.4682 0.3405 0.3220 0.3680 6.301 4 0.3405 0.4318 1050 0.1318 0.1812 0.1318 0.0690 0.1880 41.676 4 0.1318 0.1671 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01) 0.91572464 0.805 -0.673350488 -0.56809922 Bartlett's Test indicates equal variances (p = 0.77) 0.51644236 9.2103405 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 600 >600 0.10532876 0.14483158 0.02055025 0.00466886 0.046436537 2, 9 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mit!: SD 95%CL(Ex~ Skew IC05 542.24 41.38 469.24 670.91 0.3073 ICIO 611.14 30.05 502.48 672.22 -0.6344 ICI5 640.93 22.56 559.18 696.58 -0.6636 IC20 670.73 19.87 606.70 725.29 -0.4608 IC25 700.53 18.58 645.14 758.69 0.0590 IC40 792.72 16.02 744.27 831.85 -0.0054 IC50 856.49 11.73 818.67 887.00 -0.1586 Organisms obtainedfromAquatox, Inc. ppkclcr_II 0210

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I Species: Pimeohales promelas I I I I I I I I I I I I I I I STOCK I SOP AT21 - Exhibit AT21.1, revision 04-0 1-09 , Page 91 of 105

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PpKCICR Test Number: ..2.1.0 I I I CONTROL L I SOP AT21 - Exhibit AT21.1, revision 04-0 1-09

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     ,age 92 of 105
      ***                                                                         Ceriodaphnia dubia
     *fi)                                                      Chronic Reference Toxicant Control Chart
    *~       Envlronme11tal. Testing Solution$. Inc.

1.14 USEPA Control Limits (+/- 2 Standard Deviations) 1.12 1.10 1.08 1.06 1.04 1.02 2.5

- USEPA Warning and Control Limits (75 1h and 9dh Percentile CVs) u ~ 2.0 z ******************************************************************************************
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t-- 1.4 1 1 Laboratory Warning and Control Limits (10 h and 25 h Percentile CVs) 1.3 1.2 1.1 1.0 -**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-** 0.9 ****************************************************************************************** Test date

  • 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
   - - . Central Tendency (mean IC 25)
   -* -**- Warning Limits (mean IC 25 +/- SA. 10 or SA. 75)
  * *** * **** Control Limits (mean IC25 +/- SA 15 , SA. 90, or 2 Standard Deviations)

Page 93 of 105

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Ceriodaphnia dubia Chronic Reference Toxicant Control Cbart

     ~     Environmental Testing Solutions, Inc.

State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day IC25 CT s Control Umits SA.Io Warning Limits SA.lS Control Umits S,\.75 Warning Umits s...,. Control Umits cv (giL NaCI) (giL NaCI) CT-lS CT+lS CT- SA.tt CT + SA.Io CT- SA-25 CT + SA-25 CT- SA-75 CT + SA-75 CT- S.uo CT + S.uo I 07-07..00 1.06 2 08-04..00 1.01 1.07 0.00 1.06 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.00 3 08-04-00 1.09 1.01 O.ot 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.01 4 09-15..00 1.05 1.01 O.ot 1.04 1.10 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 5 10-06-00 1.08 1.01 O.ot 1.04 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.5S 0.66 0.41 1.74 O.ot 6 10-14..00 1.07 1.07 O.ot 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.2S 0.48 O.S9 l.SS 0.66 0.41 1.74 0.01 7 11-10..00 1.01 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.2S 0.48 O.S9 l.SS 0.66 0.41 1.74 O.ot 8 12-08-00 1.05 1.07 O.ot 1.04 1.10 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 O.oi 9 01-05-10 1.08 1.01 O.oi 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 10 02-02-10 1.01 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 125 0.48 0.59 1.5S 0.66 0.41 1.74 0.01 II 03-02-10 1.08 1.01 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 125 0.48 0.59 1.55 0.66 0.41 1.74 O.oi 12 04-06-10 1.05 1.07 0.01 1.05 1.10 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 O.oi 13 05-04-10 1.09 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 14 06-08-10 1.01 1.07 O.ot 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.2S 0.48 0.59 1.55 0.66 0.41 1.74 0.01 IS 07-13-10 1.01 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 125 0.48 0.59 1.55 0.66 0.41 1.74 0.01 16 08-03-10 1.06 1.07 O.oi I.OS 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 17 09-14-10 1.01 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 18 10-05-10 1.08 1.01 O.ot 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 l.S5 0.66 0.41 1.74 0.01 19 10-05-10 1.08 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.2S 0.48 0.59 1.55 0.66 0.41 1.74 0.01 20 11-02-10 1.09 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.01 Note: 7 -d 1~ = 7 -day 2S% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC,5). S = Standard deviation of the IC25 values. Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC,. values corresponding to the I Oth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint SA.Io = Standard deviation corresponding to the I o"' percentile CV. (SA 10 = 0. 08) S.us = Standard deviation corresponding to the 25th percentile CV. (SA.25 = 0.17) USEPA Control and Warning Umits SA-75 = Standard deviation corresponding to the 75th percentile CV. (SA.7s = 0.45) s...,.= Standard deviation corresponding to the 9o"' percentile CV. (SA.90 = 0.62) CV =Coefficient of variation of the IC,s values. USEPA 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Progmm. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

    **                                                                      Precision of Endpoint Measurements
   **                                                                                           Ceriodaphnia dubia Chronic Reference Toxicant Data

() Environmental Testing Solutions, Inc. Test Control Control Mean Test date CT cv CT MSD PMSD CT number Survival Reproduction for Control Mean for Control (%) (offspring/female) Reproduction (%) Reproduction (%) forPMSD (%) (offspring/female) CV(%) 1 07-07-09 100 29.9 3.3 2.2 7.2 2 08-04-09 100 30.4 30.2 4.7 4.0 2.2 7.3 7.2 3 08-04-09 100 32.0 30.8 5.1 4.4 2.2 6.9 7.1 4 09-15-09 100 31.6 31.0 5.4 4.6 2.2 7.0 7.1 5 10-06-09 100 31.4 31.1 4.3 4.6 2.4 7.6 7.2 6 10-14-09 100 33.3 31.4 3.5 4.4 2.6 7.7 7.3 7 11-10-09 100 34.0 31.8 6.2 4.6 2.6 7.7 7.3 8 12-08-09 100 35.2 32.2 4.6 4.6 2.0 5.8 7.2 9 01-05-10 100 31.1 32.1 5.8 4.8 2.3 7.3 7.2 10 02-02-10 100 31.9 32.1 3.5 4.6 2.6 8.3 7.3 11 03-02-10 100 31.0 32.0 6.3 4.8 2.4 7.7 7.3 12 04-06-10 100 32.7 32.0 6.3 4.9 2.4 7.4 7.3 13 05-04-10 100 31.0 32.0 4.6 4.9 2.3 7.4 7.3 14 06-08-10 100 33.1 32.0 6.6 5.0 2.4 7.2 7.3 15 07-13-10 100 31.6 32.0 4.8 5.0 2.3 7.2 7.3 16 08-03-10 100 28.2 31.8 4.7 5.0 1.8 6.2 7.2 17 09-14-10 100 32.9 31.8 8.7 5.2 3.0 9.2 7.4 18 10-05-10 100 30.2 31.8 7.6 5.3 2.0 6.6 7.3 19 10-05-10 100 30.8 31.7 4.0 5.3 2.2 7.2 7.3 20 11-02-10 100 30.5 31.6 4.9 5.2 2.0 6.6 7.3 Note: CV = Coefficient of variation for control reproduction. Lower CV bound determined by USEPA (lOth percentile)= 8.9"/o. Upper CV bound determined by USEPA (90th percentile) = 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (lOth percentile) = 13%. Upper PMSD bound determined by USEPA (90th percentile)= 47%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. CdNaC/CR_/10210 Page 95 of 105

            ..ET     '

Ceriodaphnia dubia

            .::) Environmental Testing Solutions. Inc.

Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements_ 35 ....***** *********************************************************** Minimum Acceptance Criteria(> 15.0 offspring per surviving female)

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Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD)

   ......... Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)

Page 96 of 105

TS Page 1 of6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species:' Ceriodaohnia dubia CdNaCICR #: \\~ Comments: water. 1400 21 1479

                                                                      }.500
                                         < 24-hours old                                                     e..

Date and times organisms were born J-;:be~tw~ee=:n=~-:-------r~--:-:--:-:=--:!:-----------1 location: Culture board: 1\-Q\* '" Daily renewal information: Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0 2 3 4 Acceptance criteria S20%

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                                                        ~ 15.0 offspring/female     ChV
                                                               <40.0%               IC SOP ATl4- Exhibit ATl4.1, revision 04-01-09
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Page 2 of6 Species: Ceriodanhnia dubia CdNaCICR #: n!> CONTROL Survival and Reproduction Data Replicate number Day 1 l ,3 4 5 6 .7 8 9 10 1 Young produced 0 0 0 () 0 c c 0 _0_ 0 Adult mortality '- '- \_..

                                                                                                   '--        \_..

l Young produced 0 0 D D () a a (\ h c... Q Adult mortality L '-- ' - \.... L '- \.... '-- \.... 3 Young produced Adult mortality 0 C> c 0 0 0 D 0

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                                                                \....    '-- '-                 \....        \_..         \.... L.                    L      L....

4 Young produced & ~ s\..... -\ '4 '-\ '-l ~ '-l "'-\ 5 Adult mortality Young produced ,,_L

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6 Young produced D 0 c 0 *0 0 0 0 0 c Adult mortality L '- '-- ' - '- L. '- '- '- \..,.

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7 Young produced 1"'\ \& \.4$. \\ Total young produced Final Adult Mortality zt; ~!>> L- ' - '- 2.C\

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Note: Adult mortality (L"' hve, D"' dead), SB = spht brood (smgle brood spbt between two days), CO= cany over (offspnng carried over with adult during transfer). l* sUTVIV. aJ an

                                                                                                                                                                . ~

600 mg NCIIL a epro uc 1on a a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced c (:) D 0 0 0 0 0 0 0 Adult mortality L '- '- '- \..... \.... '- L. '- '- 2 Young produced 0 c D 0 D c 0 0 0 L 0 Adult mortality '-- \...._ '- '- '- L. L '- '- 3 Young produced 0 0 0 0 0 0 () 0 0 0 Adult mortality L '-- '- '- '- '-- '- '- '- '- 4 Young produced 4 s 'i '-\ t- 3 c..l_ '-i ."i Adult mortality

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5 Young produced l l.. lO \1.. n. l't. 10 l\ '\ t\ \\ Adult mortality ' - '- \...._ \...... '- \._ '- \.... L '- 6 Young produced a D 0 c 0 0 0 a 0 0 Adult mortality L '-- li L.. \...._

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Note: Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO- cany over (offspnng carried over with adult during transfer). emale: SOP AT14- Exhibit AT14.1, revision 04-01-09 Page 98 of 105

Page 3 of6 Species: Ceriodaphnia duhia CdNaCICR #: \\ ~ 800 mg Na CI/L S UFVIV . a/ andReproduct1on D ata Replicate number Day 1 2 ,J 4 s 6 7 8 9 10 1 Young produced 0 0 c 0 0 c D 0 _C c Adult mortality L. '-- "- L.. '- L.. \..._

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Final Adult MortaHb' \. \ \. \. L.. ' - '- '- L \..... Note: Adult mortality (L = bve, D =dead), SB = spht brood (smgle brood spht between two days}, CO= carry over (offipnng carried over with adult during transfer). II Concentration:

                                                                                                      % Mortality:                          Ql ..

Mean Offspring/Female: l.'l.i

                                                                                                      % Reduction from Control:            'l..?.t7..
  • I 1000 mg NCIIL a S urvtva. I an dR eproduct"ron Data Reolieate number i

Day 1 l 3 4 5 6 7 8 9 10 1 Young produced (:) b b 0 _D _() 0 0 0 _Q_ Adult mortality \..... L '-- \..._ '- L_

                                                    '-      '--      \.....                              \._.

l Young produced _C) 0 D 0 D D D. D 0 ~ Adult mortaUty L L. '- '- \._

                                                                                                          '-        L..    '-       L.           L.

3 Young produced 0 0 0 0 0 _0 0 0 0 o* Adult mortality L. \.._ l.... '-- \..... L L \._ '- c..... 4 Young produced '-\ --'i 'i .s* '-( .3 ~ '-i "\ ~ Adult mortality

                                                     '-       L         L         L                L       '-        L        L.      '-           L 5        Young produced           10     1\          lC          10            "\       \'l.. IC       lC     q             l\

Adult mortaUty L '-- '- L- \..._ L '-- L. '- L 6 Young produced 0 0 0 c c 0 c 0 _Q 0

                                                                                                                                                ~

I Adult mortality

                                                    '-      \.._.     \.....       \......      '-        '-        '-     '-      '-

I I I 7 Young produced Total young produced

                                                   \~
                                                   "2.~
                                                          '~
                                                            '1.t l\o
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                                                                                                         \'"\
                                                                                                          ~1..
                                                                                                                  *~
                                                                                                                    '2.b lb
                                                                                                                           ~b tt-2.'1 IS 1.'\

I Final Adult Mortality L... \..._

                                                                      '-        \.              L.                 '-      '-        \.....        \.._

Note: Adult mortality (L = hve, D =dead), SB =split brood (smgle brood spht between two days), CO- carry over (offipnng carried over with adult during transfer). Concentration: I'!

;i
                                                                                                       % Mortality:

Mean Offspring/Female:

                                                                                                       % Reduction from Control:

0_1. u.~ s.1.7. SOP AT14- Exhibit ATl4.1, revision 04-01-09 lage of 99 I 05

Page4 of6 Species: Ceriodaphnia dubia CdNaCICR #: (l ~ 1200 M! Na CIIL Survm 'a/ andRepro uction Data Replicate number Day I 2 tJ 4 5 6 7 8 9 10 1 Young produced 0 _0 0 0 (:} 0 0 _Q 0 0 Adult mortality L.. \..... \...... ..__ '-- L.. '- '- \...... "- 2 Young produced a 0 0 Q D 0 a _C) c c Adult mortality L. '-- ~ \.- \.._.

                                                                                                                    '--          '--         '-          L            '--

3 Young produced ("") 0 c 0 c 0 _0 0 0 Q Adult mortality \..._ L \.._.

                                                                                       '-             \..._         L-        '-           \...     \.._.            '--

4 Young produced '2.. ~ ~ 0 \ '2..

                                                                                                                                                   '\...          ~

Adult mortality \...... \....... '-- '--- \.......

                                                                                                      '-          \.......                            \....          \.......

5 Young produced g 10

                                                                 ..__       la            i          ~             s                        ~     _S_             ~

Adult mortality

                                                     '-                       \.._.      \.......    ,\.......    '--            '-          ~         \...          L.

6 Young produced 0 _C_ 0 0 0 D _Q 0 C) 0 I 7 Adult mortality Young produced Total young produced

                                                   '\
                                                              ~           .&          5             ,           ~
                                                                                                                  \.._       '--

5 5

                                                                                                                                                   .a L..

l.. l'l \I\ L1 - \~ '\ \\e \\. \~ Final Adult Mortality '- '-- '-- '-- '- \...... '- '- \..... "- Note: Adult mortality (L = hve, D =dead), SB- spilt brood (smgle brood spht between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration:

                                                                                                            % Mortality:                                    01.

Mean Offspring{female: .~.~

                                                                                                            % Reduction from Control:                     'f1_,q1.

1400 M! NCIIL a Surv1va ' I andReproduct'zon Data Replicate number Dav 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 0 (") 0 0 0 Adult mortality L '-- '- \.._ '- '-- \,_ 2 Young produced 0 D 0 0 a D c 0 0 *a_ Adult mortality '- '-- '-- L \... \,... L '- '-- '-- 3 Young produced 0 (!) 0 a _Q 0 0 _Q __Q_ 0 Adult mortality L. \...... '- L L L '- L L 4 Young produced l 0 '1... 1 0 0 0 0 l 5 Adult mortality Young produced L-c.{

2. s L. '-
                                                                                      .s             ,

L.. '-- _3_

                                                                                                                               \.....
                                                                                                                              --':!_        ~
                                                                                                                                                  ~
                                                                                                                                                                   ~
                                                                                                                                                                     \,_.

I '-- b. \_. Adult mortality L '-- '-- '-- \,__

                                                                                                                                                      '--             \..-

6 Young produced 0 0 0 a a 'L \ \ c lS_ Adult mortality

                                                   '-         \._          '-              L           '-           '--         1             -~       L             '--

I 7 Young produced Total young produced , 2 5 0

                                                                         ., 0          6 0

IC s 0 lo 0 L.\ ~ c 2.. Final Adult Mortality '-- '-- '- '- _ ...... ~- '- \.... \..... I Note: Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO- carry over (offspnng carried over with adult during transfer). Concentration: I  % Mortalfu': Mean Offspring/Female:

                                                                                                               % Reduction from Control:

107.

                                                                                                                                                              !L""'-LUl.

Jo.~'7a t..ol. SOP AT14- Exhibit AT14.1, revision 04-01-09 tage 100 of105

~ ~ ~ ~ ~

     ..ETS v.

c~'*'***

  • ',,~* . :*": . :,~
                             *      ,:,*,      6~,-;,:}',S>                     Verification of Ceriodaphnia Reproduction Totals
     ~        Environmental Tesdng Solutions, Inc.

Control 1000 mg NaCI/L Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 3 3 5 4 4 4 4 3 4 4 38 4 4 4 4 5 4 3 3 4 4 3 38 5 12 12 10 12 10 10 12 13 10 11 112 I 5 10 II 10 10 9 12 10 10 9 11 102 6 0 0 0 0 0 Q 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 14 18 14 16 15 15 16 14 17 16 155 7 15 13 16 14 14 17 13 16 16 15 149 Total 29 33 29 32 29 29 32 30 31 31 305 Total 29 28 30 29 27 32 26 30 29 29 289 600 mg NaCI/L 1200 mg NaCIIL Replicate number Replieate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0. 0 0 0 0 0 I 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 4 6 3 4 4 4 4 42 4 2 3 3 3 1 2 4 2 2 3 25 5 12 10 12 12 12 10 11 11 II 11 112 5 8 10 6 8 5 5 7 9 5 8 71 I 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 15 15 18 17 17 14 16 16 17 15 160 7 9 6 8 5 7 4 5 5 8 6 63

               .___]:'otal           31     30        34    33    35     27  31 31   32   30    314             Total 19  19  17 16    13     11  16 16 15 17  159 800 mg NaCIIL                                                                                 1400 mg NaCI/L Reolicate number                                                     Replicate number Day                                                                   Total            Day                                           Total 1      2         3     4     5      6   7  8    9   10                           1   2   3  4     5      6   7  8  9 10 I         0      0         0     0     0      0   0  0    0    0      0               1    0   0   0  0     0      0   0  0  0  0   0 2         0      0         0     0     0      0   0  0    0    0      0               2    0   0   0  0     0      0   0  0  0  0   0 3         0      0         0     0     0      0   0  0    0    0      0              3     0   0   0  0     0      0   0  0  0  0   0 4         4      4         3     4     5      4   4  5    4    4     41               4    1   0   2  I     0      0   2  0  0  I   7 5         9     10        12    11     12    10  10 10   10   13    107               5    4   2   5  5     7      3   4  3  3  4   40 6         0      0         0     0     0      0   0  0    0    0      0               6    0   3   0  0     0      2   0  I  0  0    6 7        I6     16        15    13     17    15  16 14   14   14    150               7    2   0   0  0     3      0   0  0  0  2    7 Total        29     30        30    28    34     29  30 29   28   31    298             Total  7   5   7  6    10      5   6  4  3  7   60
                                                                                                                              -\-
~

~~

~

~....

~

ETS Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 e

     ~      Environmental Testing Solutions, Inc.

Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #113 Test dates: November 02-09, 2010

                                                                                                                                                                                                   ,- ~~,~--
                                                                                                                                                                                                   '--"I Concentration                                                           Replicate number                                                     Survival     Average reproduction        Coeflk:ielltof   Perceat red*ctioo from (mg/1.. NaCI)                                                                                                                                  (%).       (offspring/female)         varilllioo (%)        control(%)

1 2 3 4 5 6 7 8 9 10 Control 29 33 29 32 29 29 32 30 31 31 100 30.5 4.9 Not applicable 600 31 30 34 33 35 27 31 31 32 30 100 31.4 7.2 -3.0 800 29 30 30 28 34 29 30 29 28 31 100 29.8 S.9 2.3 1000 29 28 30 29 27 32 26 30 29 29 100 28.9 5.8 5.2 1200 19 19 17 16 13 II 16 16 15 17 100 15.9 15.5 47.9 1400 7 5 7 6 10 5 6 4 3 7 90 6.0 32.4 80.3 Dunnett's MSD value: 2.008 MSD = Minimum Significant Difference PMSD: 6.6 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. 111 Lower PMSD bound determined by USEPA (10 percentile) = 13%. Upper PMSD bound determined by USEPA (9rfl' percentile)= 41%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 2001 a, 200 I b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B..() 1-004 and EPA-821-B-0 1..005. US Environmental Protection Agency, Cincinnati, OH.

                                                                                                       **-***--1---

~ ~ ~ ~ ~ iETS

     ~    Environmental Tesdng Solutions, Inc.

Statistical Analyses Cerioda2hnia Survival and ReJ?!oduction Test-R!:J!roduction Start Date: 11/2/2010 TestiD: CdNaCICR SampleiD: REF-RefToxicant End Date: 11/9/2010 LabiD: ETS-Envir. Testing Sol. Sample Type: NACL-Sodiwn chloride Sample Date: Protocol: FWCHR.-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mg/L 1 2 3 4 5 6 7 8 9 10 D-Control 29.000 33.000 29.000 32.000 29.000 29.000 32.000 30.000 31.000 31.000 600 31.000 30.000 34.000 33.000 35.000 27.000 31.000 31.-000 32.000 30.000 800 29.000 30.000 30.000 28.000 34.000 29.000 30.000 29.000 28.000 31.000 1000 29.000 28.000 30.000 29.000 27.000 32.000 26.000 30.000 29.000 29.000 1200 19.000 19.000 17.000 16.000 13.000 11.000 16.000 16.000 15.000 17.000 1400 7.000 5.000 7.000 6.000 10.000 5.000 6.000 4.000 3.000 7.000 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean 0-Control 30.500 1.0000 30.500 29.000 33.000 4.948 10 30.950 1.0000 600 31.400 1.0295 31.400 27.000 35.000 7.231 10 -1.025 2.287 2.008 30.950 1.0000 800 29.800 0.9770 29.800 28.000 34.000 5.876 10 0.797 2.287 2.008 29.800 0.9628 1000 28.900 0.9475 28.900 26.000 32.000 5.755 10 1.822 2.287 2.008 28.900 0.9338

             *1200         15.900         0.5213         15.900       11.000        19.000         15.533              10         16.622          2.287       2.008      15.900        0.5137
             *1400           6.000        0.1967          6.000        3.000        10.000         32.394              10         27.894          2.287       2.008       6.000        0.1939 Auxiliary Tests                                                                                                Statistic                Critical                  Skew          Kurt Kolmogorov D Test indicates normal distribution (p > 0.01)                                                 0.71356082                    1.035                 -0.001903    0.42585914 Bartlett's Test indicates equal variances (p = 0.68)                                                       3.11474943                 15.0862722 Hypothesis Test (l-tai1, 0.05)                  NOEC          LOEC           ChV            TU                 MSDu       MSDp           MSB          MSE        F-Prob          df Dunnett's Test                                    1000         1200      1095.44512                        2.00847119 0.06585151        1087.79    3.85740741   2.2E-37         5,54 Treatments vs D-Contro1 Linear Interpolation (200 Resamples)

Point mli!: so 95%CL Skew lC05 888.333333 90.6802548 737.758094 1007.27887 0.1599 IC10 1016.07692 12.8168457 973.914706 1029.47383 -2.4643 IC15 1039.88462 8.00823247 1021.89463 1052.38648 -0.1932 IC20 1063.69231 7.77779255 1047.59504 1077.41926 -0.1892 IC25 1087.5 7.87833738 1072.02296 ll02.54717 -0.1740 IC40 1158.92308 9.90261917 1142.45899 ll79.30357 -0.0982 IC50 1208.58586 13.0946065 1187.65139 1233.9306 -0.1607 CdNaC/CR 110210

            . .,. . T~ ~

Page 5 of6 iE_ J ..... , *. 5

       ~ lllvlranmtntlllallntSolutlon~lnc.

Species: Ceriodaohnia dubia CdNaCICR #: 1\~ CONTROL

                 ~OOmgNaCVL 800mgNaCVL

~ I I I I \ I STOCK I ~Page 104 of lOS SOP AT14- Exhibit ATl4.1, revision 04-01-09

           *ers 9    '
            ) ***.**..       :;*      ....
          ~EnvlraniMfttloiTI:stfngSolutlon.. lllc.

Page 6 of6 Species: Ceriodaphnia dubia CdNaCICR #: l \ !> CONTROL 600 mgNaCVL 'I'* l '

 .Page 105 ofl05 SOP ATl~. if~.~bitATI4.1, revision 04-01-09

PERMITTEE NAME/ADDRESS (Include Facility Name/location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES} MAJOR Form Approved. Nam~ -W~SEOUOYA~UCLEARP~~ --- DISCHARGE MONITORING REPORT (DMR} (SUBR 01) OMB No. 2040-0004 Address _p.o._e_ox 200Q_ _ _ _ _ _ _ _ _ _ _ _ --- ~INTEROFFIC~B-2A-SQ~ ------- 101 G -~ F- FINAL ---~ ODDY~AISY . TN238L Facili~ _NA~~UOYA~UCLEARPLANL _ _ _ _ ______ _ 1---------i I DISCHARGE NUMBER DIFFUSER DISCHARGE v;~* I ~~ r~r~: r!rt ~~ I ~"t I Locat~~AMIITO~OUNTI_ _ _ _ _ _ _ _ _ _ EFFLUENT From I *** NO DISCHARGE D ... ATIN : stephanie A. Howard NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY< SAMPLE TYPE AVERAGE MAXIMUM I I ANALYSIS UN II:> I MINIMUM AVERAGE MAXIMUM UNITS CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT

                                                                        ********                   ********                ..       I      ********           0.014             0.028              19         0      31 I 30 I GRAB 50060         0     0                          PERMIT
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1 62 I ******** ******** .. 0 30 I 30 I CALCTD 82234 0 0 PERMIT REQUIREMENT

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                                                                                                                                                     ~-

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my I TELEPHONE I DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D . Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site

  • Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 843-7001 I 10 I 12 I 08 and complete. I am aware that there are significant penalties for submitting false information, f-- - -- - - - - - - - - - - - - - -- -_jincluding the possibility of fine and imprisonment for knowing violations.

TIPED OR PRINTED NUMBER I YEAR I MO I DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No other BCTP chemical injections besides Towerbrom 960 for November 2010. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINAT ION SYSTEM (NPOESJ MAJOR Form Approved . DISCHARGE MONITORING REPORT (OMR) Nam~-W~SEOUOYA~UCLEARP~~ --- (SUBR 01) OMB No. 2040-0004 Address _p_.Q.__B_OX 2.000_ _ _ _ _ _ _ _ _ _ _ _ 101--T -l F- FINAL --- ~ INTEROFFICESB-2A-SQ ~ ------- ---~ ODDY~AISY.TN~3BL Facili~_NA~ EQUOYA~UCLEARPLAN~ ---- ______ _ ~==:::::~:::::::~===~ I DISCHARGE NUMBE R] BIOMONITORING FOR OUTFALL 101 Locatio~AMI~O~OUNTI ATTN : stephanie A. Howard ~~ r~r~: n:t't'l ~"; 1 1 EFFLUENT

                                                                                                                                                                              *** NO DISCHARGE          D      ***

X~ --- ~ - -;=-----~~=---r-~--1 PARAMETER QUANTITY OR LOADING QUALITI OR CONCENTRATION SAMPLE TYPE I ANALYSIS AV~KAl;;~ I MAXIMUM I UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ******** I ******** I ** >1 00.0 ******** ******** 23 0 1 I 180 I COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 PERMIT

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my I TELEPHONE I DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 1 - - - - - - - - - - - - - - - - - - - - lI 423 843-7001 I 10 I 12 I 08 and complete. I am aware that there are significant penalties for submitting false information , 1=-r SIGNATURE OF PRINCIPAL EXECUTIVE 1 - -- -- - - - - - - - - - -- - - - - --lincluding the possibility of fi ne and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER I YEAR I MO I DAY TYPED OR PRINTED co COMMENTS AND EXPLANATION OF ANY VIOLATIONS fReference all attachment s here Toxicity sampling began on October 31 and ended on November 5. See attached report. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilitv Name/L ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. Nam~ -~~SEOUOYA~UCLEARP~~ --- DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 Address _p....Q.JtOX 2.Q...OO_ _ _ _ _ _ _ _ _ _ _ _ ---~ INTEROFFIC~B-2A - SQ~ - ------ ---~@DY~AI~TN~38L ______ _ F--* TN0026450 PERMIT NUMBER II 103 G DISCHARGE NUMBER I (SUBR 01) F- FINAL LOW VOL. WASTE TREATMENT POND r4"'"" r=t Facili~ _NA~EQUOYA~UCLEARPLAN L _ _ _ _ Locatio ~ AMI~ONCOUNTI_ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN : stephanie A. Howard From 10 I 11 I YEAR MO 01 To 10 MO 11 I ""' 30 I

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NOTE: Read instructions before completinQ this form X PARAMETER QUANTITY OR LOADING OUALITI OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE MEASUREMENT

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                                                                                                                                                                                                             ..                SEE        TOTALZ EFFLUENT GROSS VALUE                                                         MOAVG                    DAILYMX                                                                                                                PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER              I Certify under penalty of law that this document and all attachments were prepared under my                                                          TELEPHONE                    DATE
                                                                                                                                                           '2,.;.!.c:ES'"'

direction or supervision in accordance with a system designed to aSsure that qualified personnel M ichael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information. the information submitted is , to the best of my knowledge and belief, true. accurate, 423 I 843-7001 10 12 08 Sequoyah Site V ice President and complete. I am aware that there are significant penalties for submitting false information. SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing vio lations. TIPED OR PRINTED OFFICER OR AUTHORIZED AGENT

                                                                                                                                                                                                     ~~~~ I      NUMBER        YEAR      MO    DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                     (Ref erence all attachments h ere EPA Form 3320-1 (REV 3/99)               Pre vious editions may be used                                                                                                                                                    Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NAT IONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) MAJOR Form Approved. Nam~ -~~SEOUOYA~UCLEARP~~ --- DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 Address _p.O._!!_OX 2000_ _ _ _ _ _ _ _ _ _ _ _ (SUBR 01) 5 I P:R~~~~~~ B~R II ~:M~ER I --- ~ INTEROFFICESB - 2A - SQ ~ ------- F - FINAL --- ~ODDY~AISY . TN23BL ______ _ DISCHARG: METAL CLEANING WASTE POND Facili~ _NA~ EQUOYA~UCLEARPLAN C _ _ _ _ Locati o ~AMUO~@N~---------- ATIN : stephanie A. Howard From I YEAR 10 IM 11 Or:vr*ING01 To FJ10 MQ 11 IO30 AY I EFFLUENT

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NOTE: Read instructions before completinQ this form X PARAMETER QUANTITY OR LOADING OUALI~ OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE **** **** ******** MEASUREMENT 12 00400 1 0 0 PERMIT

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                                                                                                                               ......         ********            ********             30              MG/L                DAI LY     COMPOS REQUIREMENT E FFLUENT GROSS VALUE DAILY MX OIL AND GREASE                                    SAMPLE                                               ********

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                                                                                                                                              ********            ********             15              MG/L                DAILY        GRAB EFFLUENT GROSS VALUE DAILYMX PHOSPHORUS, TOTAL (AS P)                          SAMPLE                     ********                  ********                                 ********

MEASUREMENT ** ******** 19 00665 1 0 0 PERMIT REQUIREMENT

                                                                                                                               .....           ********          ********             1.0              MG/L                DAILY      COMPOS EFFLUENT GROSS VALUE DAILYMX COPPER, TOTAL (AS CU)                             SAMPLE                     ********                  ******... *                              ********           ********

MEASUREMENT ** 19 01042 1 0 0 PERMIT ******** ******** **** ******** ******** REQUIREMENT 1.0 MG/L DAILY COMPOS EFFLUENT GROSS VALUE DAILYMX IRON, TOTAL (AS FE) SAMPLE ******** ******** ******** ******** MEASUREMENT ** 19 01045 1 0 0 PERMIT ******** ******** **** ******** ******** 1.0 MG/L DAILY COMPOS REQUIREMENT EFFLUENT GROSS VALUE DAILYMX FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** TREATMENT PLANT MEASUREMENT 03 ** 50050 1 0 0 PERMIT REQUIR EMENT REPORT REPORT MGD ******** ******** ******** .... DAILY CALCTD EFFLUENT GROSS VALUE MOAVG DAILY MX r:J5f.11Q:.,, NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law thai this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel TELEPHONE DATE M ichael D . Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or lhose persons directly responsible for gathering the information, the information submitted is , to the besl of my knowledge and belief, true, accurate, 423 843-7001 10 12 08 Sequoyah S ite V ice President and complete. I am aware that there are significant penalties for submitting fa lse information, SIGNATURE OF PRINCIPAL EXECUTIVE I

            ~PED    OR PRINTED inc luding the possibility of fine and imprisonment for knowing viola tions.

OFFICER OR AUTHORIZED AGENT AREA CODE l NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved . NamL _~~SEOUOYA~UCLEARP~~ --- DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 Address _p.O ._ilOX 200Q_ _ _ _ _ _ _ _ _ _ _ _ (SUBR 01) --- ~ INTEROF~UB -2A-SQ~ ------- --- ~~DY~AISY . TN~38L ______ _

                                                                                                                                             ~            110 G       I F - FINAL DISCHARGE NUMBER         RECYCLED COOLING WATER Facili~ _NA~EQ~YA~UCLEARPLANT _ _ _ _ _

I I EFFLUENT I MONITORING PERIOD_ j x;~R MC 11 I ~~~ I To Ix;~R I 11 I ~~ MO

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L- . III ..,UUVLIVII;;) LJCIVI't:;; VVIIItJIC'llll!-4 liiiO:. lU ll II . PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** 04 ******** CENTIGRADE MEASUREMENT 04 00010 z 0 0 PERMIT ******** ******** DEGC ******** ******** 38.3 DEGC DAILY GRAB-4 REQUIREMENT INSTREAM MONITORING PH SAMPLE MEASUREMENT DAILYMX 12 00400 1 0 0 PERMIT **** su REQUIREMENT

                                                                        ********                  ********                                    6.0             ********                   9.0                                                     WEEKLY                     GRAB EFFLUENT GROSS VALUE MINIMUM SOLIDS, TOTAL SUSPENDED                        SAMPLE MEASUREMENT MAXIMUM 19 00530     1    0     0                         PERMIT REQUIREMENT
                                                                        ********                  ********                 ****            ********           ********                    30                        MG/L                            DAILY                 COMPOS EFFLUENT GROSS VALUE OIL AND GREASE                                 SAMPLE MEASUREMENT DAILY MX 19 00556     1    0     0                         PERMIT                    ********                  ********                 ****            ********           ********

REQUIREMENT 15 MG/L DAILY GRAB EFFLUENT GROSS VALUE DAILY MX FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT 03

                                                                                                                                           ********           ********               ********                          ..                                                          I 50050     1    0     0                         PERMIT REQUIREMENT REPORT                    REPORT                   MGD               ********           ********               ********                        ....                           DAILY                 CALCTD EFFLUENT GROSS VALUE                                                    MOAVG                    DAILYMX CHLORINE, TOTAL RESIDUAL                       SAMPLE MEASUREMENT 19 50060     1    0     0                          PERMIT REQUIREMENT
                                                                        ********                  ********                 ....            ********           ********                  0.10                        MG/L                         WEEKLY                   GRAB-4 EFFLUENT GROSS VALUE DAILYMX SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER        I Certify under penalty of law that this document and all attachments were prepared under my sit~viWdent direction or supervision in accordance with a system designed to assure that qualified personnel TELEPHONE                                        DATE Michael D. Skaggs                   properly galher and evaluate the informal ion submitted. Based on my inquiry of the person or persons who manage lhe system, or those persons direclly responsible for gathering lhe informalion, lhe informalion submitted is . to the besl of my knowledge and belief, true, accurate,     sequoyah Sequoyah Site Vice President                                                                                                                                                                            423           843-7001                    10             12       08 and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing vio lations.

SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS rReference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved . Nam~ -N~SEOUOYA~UCLEARP~~ --- DISCHARGE MONITORING REPORT (OMR) OMS No. 2040-0004 (SUBR 01) Address _p .O.J!..OX 2.m_ _ ____ _ ____ _ 5 I p~%~~~~! e~R ~~M:ER I - -- ~ INTEROF~UB-2A - SQ~ ------- 1 F - FINAL --- ~ODDY~AISY . TN~~------ - - II r:?r*G r t DISCHARGE RECYCLED COOLING WATER Facili~ _NA~EQUOYA~~lEARPlANL _ _ _ _ locat~JAMILTONCOUNIT_ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN : stephanie A. Howard From 1 10 """I MO 11 01 To 10 MQ 11 1 * ., 1 30 NO DISCHARGE I XX I *** NOTE: Read instructions before completinQ this form X PARAMETER OUANTrrY OR LOADING OUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE OF I EX TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR CERIODAPHNIA SAMPLE MEASUREMENT

                                                                         ********                   ********                ..                                  ********          ********            23 TRP3B     1    0      0                         PERMIT REQUIREMENT
                                                                         ********                  ********                ****               45.2             ********           ********        PERCENT                SEMI       COMPOS.

EFFLUENT GROSS VALUE I MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** ** ******** ******** PIMEPHALES MEASUREMENT 23 TRP6C 1 0 0 PERMIT REQUIREMENT

                                                                         ********                  ********                ****               45.2             ********           ********        PERCENT                SEMI       COMPOS EFFLUENT GROSS VALUE MINIMUM                                                                       ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
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r::I~~eot NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel TELEPHONE DATE Michael D . Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is . to the best of my knowledge and belief. true. accurate. 843-7001 10 12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information. 423 I 08 including the possibility of fine and imprisonment for knowing violations. SIGNATURE OF PRINCIPAl EXECUTIVE ITPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA l NUMBER YEAR MO DAY CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No D ischarge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Na~--~~SEOUOYA~UCLEARPLA~ --- DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Address _P..Q .JtOX 200Q_ _ _ _ _ _ _ _ _ _ _ _ ---~ INTEROF~UB-2A - SQ~ -- -- -- - I -u-116 G F- FINAL - -- ~ODDY~AISY . TN2~~ -- ----- Facili~ _NA~EQ~YA~UCLEARPLANL __ _ _ DISCHARGE NUMBER I BACKWASH Locatio~AMI~~OUNTI_ _ _ ATIN: Stephanie A _ Howard From Iv*~~ 10 MO r~RING 11 01 To Fl 10 MO 11 I OAV 30 I EFFLUENT NO DISCHARGE D ... NOTE: Read instructions before completinQ this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO_ FREQUENCY SAMPLE EX OF TIPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE ******** ******** ******** MEASUREMENT ** ******** 0 0 1 I 30 VISUAL 9A 01345 1 0 0 PERMIT REQUIREMENT

                                                                         ********                  ********                ""'***           ********              ********       REPORT            PASS=O                 SEE       VISUAL EFFLUENT GROSS VALUE                                                                                                                                                                                 FAIL=1 MOTOTAL                                 PERMIT OIL AND GREASE VISUAL                           SAMPLE                    ********                       0                                   ********              ********

MEASUREMENT 94 ******** ** 0 1 I 30 VISUAL 84066 1 0 0 PERMIT REQUIREMENT

                                                                         ********                REPORT                 YES=1               ********              ********        ********            ****                SEE       VISUAL EFFLUENT GROSS VALUE                                                                                                      NO=O MOTOTAL                                                                                                                 PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT r:J.~. ~"'"'

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my Michael D . Skaggs direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or TELEPHONE DATE I persons who manage the system, or lhose persons directly responsible for gathering the information. the information submitted is , to the best of my knowledge and belief. true. accurate. 423 843-7001 10 12 Sequoyah Site Vice President 08 and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE I

                                                                                                                                                                                               ~~~ I TIPED OR PRINTED                                                                                                                               OFFICER OR AUTHORIZED AGENT                     NUMBER        YEAR      MO    DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                (Reference all attachments here Operations performs visual inspections for floating debris and oil and grease during all backwashes.

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved . NamL _~~SEOUOYA~UCLEARP~~ --- DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Address _p..Q.JtOX 200Q_ _ _ _ ____ ___ _ --- ~INTEROFFICUB-2A-SQ~ ------- --- ~ODDY~AISY.TN238L _ _ _ _ _ _ _ IDISCHARGE117 G I NUMBER F- FINAL BACKWASH Facili~ _NA~EQUOYA~UCLEARPLAN~ ---- Locatio~AMI~~~~Nn_ ATIN : stephanie A. Howard From 10 I m:l MQ 11 rWJRING 01 To Fl MO I 10 11 PO> 30 I EFFLUENT NO DISCHARGE D *** NOTE: Read instructions before completinQ this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION FREQUENCY NO. SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE ******** ******** ******** ********

                                                                                                                            **                                                    0                           0      1 I 30      VISUAL MEASUREMENT                                                                                                                                             9A 01345     1    0    0                           PERMIT REQUIREMENT
                                                                         ********                 ********                 ****            ********           ********        REPORT            PASS=O                SEE        VISUAL EFFLUENT GROSS VALUE                                                                                                                                                                              FAIL=1 MOTOTAL                                 PERMIT OIL AND GREASE VISUAL                           SAMPLE MEASUREMENT
                                                                         ********                       0                  94               ********          ********         ********              **       0      1 I 30      VISUAL 84066     1    0    0                           PERMIT REQUIREMENT
                                                                         ********               REPORT                  YES=1              ********           ********         ********
                                                                                                                                                                                                   ....               SEE        VISUAL EFFLUENT GROSS VALUE                                                                                                      NO=O MOTOTAL                                                                                                              PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsibl e for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 10 12 08 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE l TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINAT ION SYSTEM (NPDES) MAJOR Form Approved . DISCHARGE MONITORING REPORT (DMR) NamL _~~SEOUOYA~UCLEARP~~ --- (SUBR 01) OMB No. 2040-0004 Address _p.O._!!_OX 200Q_ _ _ _ _ _ _ _ _ _ _ _ ---~ INTEROFFIC~B-2A

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--- ~ODDY~AISY . TN23BL I TN0026450 PERMIT NUMBER II 118 G DISCHARGE NUMBER I F - FINAL WASTEWATER & STORM WATER Facili~ _NA~EQ~YA~UCLE~PLANL _ _ _ _ Locatio~AMI~O~OUNTI_ _ _ _ _ _ _ _ _ _ ATTN: Stephanie A. Howard From I,10... I 11 MQ r~r*"01 To F10 t 11 I 30 I MQ PAY EFFLUENT NO DISCHARGE IXX I *** NOTE: Read instructions before completinQ this form. X PARAMETER QUANTITY OR LOADING OUALITI OR CONCENTRATION NO. FREQUENCY SAMPLE I EX OF TIPE I AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT

                                                                             ********                   ********                ..                                   ********           ********            19 00300       1    0     0                            PERMIT REQUIREMENT
                                                                                                                               ....                   2.0            ********           ********          MG/L                  TWICE/             GRAB EFFLUENT GROSS VALUE                                                                                                                          DAILY MN                                                                           WEEK SOLIDS, TOTAL SUSPENDED                           SAMPLE MEASUREMENT
                                                                             ********                   ********                ..                ********           ********                               19 00530      1    0     0                            PERMIT                   ********                   ********                                  ********           ********              100             MG/L                 TWICE/             GRAB REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                                                                 DAILYMX                                     WEEK SOLIDS, SETTLEABLE                                SAMPLE MEASUREMENT
                                                                             ********                   ********                ..                ********           ********                               25 00545      1    0     0                            PERMIT                   ********                   ********               ****               ********           ********              1.0             MUL                    ONCE/             GRAB REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                                                                 DAILYMX                                   MONTH FLOW, IN CONDUIT OR THRU                           SAMPLE 03                ********           ********            ********           ..

TREATMENT PLANT MEASUREMENT 50050 1 0 0 PERMIT REPORT REPORT MGD ******** ******** ******** ONCE/ ESTIMA REQUIREMENT EFFLUENT GROSS VALUE MOAVO DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMP LE MEASUREMENT PERMIT REQUIREMENT t::LZ~:~deot NAME/ TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law lhallhis documenl and all attachmenls were prepared under my I TELEPHONE I DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs prope~y gather and evaluate the information submitled. Based on my inquiry of the person or persons who manage l he syslem, or those persons direclly responsible for galhering l he informalion, lhe informalion submitted is , lo the best of my knowledge and belief, lrue, accural e, - 08 Sequoyah Site Vice President and complete. I am aware that th ere are significant penalties for submitting fa lse information, SIGNATURE OF PRINCIPAL EXECUTIVE f - - - - - - - - - - - -- - - - - - - - - - 1 including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT DAY TIP ED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Pre vious editions may be used Page 1 of 1

S58 110110 800- NPDES CORRESPONDENCE January 10, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR DECEMBER 2010 Enclosed is the December 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 12/18/2010 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, n~ k gs Site Vice Presid t Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control ATTN: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc: B. E. Brickhouse, LP 5U-C A A Ray, WT 11A-K D. A Day, POB 2B-SQN G. R. Signer, WT 6A-K S. A Howard, OPS 5N-SQN M. D. Skaggs, OPS 4A-SQN K. Langdon, POB 2B-SQN B. A Wetzel, OPS 4A-SQN D. B. Nida, LP 5U-C K. M. Hodges (EDMS), LP 2V-C

I 1 l 1£ Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 January 10, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR DECEMBER 2010 Enclosed is the December 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 12/18/2010 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, r~~~ Michael D. SkJg Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) Nam~-~~~OUOYA~UCLE~P~~ -- - (SUBR 01) OMB No 2040-0004 AdQ[ess _p....Q.JtOX 2000_ _ _ _ _ _ _ _ _ _ _ _ ---~IN~~~~~~~L ---~ODDY~AI~ffl2~---- -- - - Fa~~-~~~uom~~~R~~L _ _ _ _ I -- p:~~!~~ J I::HARO:~~M~ER I F- FINAL DIFFUSER DISCHARGE ~~~~AMILN~~-- - -- -- --- ATTN: Stephanie A. Howard From I YEAR 10 \1 2 MO t~TriNG p~ 01 To !10 I 31I MQ 12 PAY EFFLUENT

                                                                                                                                                                              ***  NO DISCHARGE          D ...

NOTE: Read instructions before completin!l this form X PARAMETER QUANTrrY OR LOADING OUALrrY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. CENTIGRADE SAMPLE MEASUREMENT

                                                                                                                              -               ********           ********             14.9               04        0      31 I 31      MODELD 00010     z      0    0                           PERMIT
                                                                           **'******                  ********               ****             ********           ********             30.5            DEG.C.                SEE         CKREQ REQUIREMENT INSTREAM MONITORING TEMPERATURE, WATER DEG.

CENTIGRADE SAMPLE MEASUREMENT DAILYMX 27.9 04 0 PERMIT 31 I 31 RCORDR 00010 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT

                                                                           ********                   ********               *-*              ********           ********          REPORT DAILYMX DEG.C.                SEE PERMIT CKREQ TEMP. DIFF. BETWEEN SAMP. &

UPSTRM DEG.C SAMPLE MEASUREMENT

                                                                                                                                               ********          ********              3.3               04        0      31 I 31       CALCTD 00016     1      w    0                           PERMIT
                                                                            ********                  ********                                ********           ********              5.0            DEG.C .            CONTIN         CALCTD REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                                                               DAILYMX                                  uous PH                                               SAMPLE MEASUREMENT
                                                                                                                               -                  7.8            ********              8.1               12        0       5 I 31         GRAB 00400     1      0    0                            PERMIT                    ********                  ********                ****                6.0            ********              9.0               su             WEEKLY            GRAB REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                         MINIMUM                               MAXIMUM SOLIDS, TOTAL SUSPENDED                           SAMPLE MEASUREMENT
                                                                                                                               -               ********              4                  4                19        0       1 I 31         GRAB 00530     1      0 EFFLUENT GROSS VALUE 0                            PERMIT REQUIREMENT
                                                                            ********                  ********                **-              ********             30 MOAVG 100 DAILYMX MG/L           MONTHLY            GRAB OIL AND GREASE                                    SAMPLE MEASUREMENT
                                                                                                                               -               ********              <5                 <5               19        0       1 I 31         GRAB 00556     1     0     0                           PERMIT REQUIREMENT
                                                                            ********                  ********                -**              ********              15                 20              MG/L           MONTHLY            GRAB EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT
                                                                            ********                    1661                   03 MOAVO DAILYMX
                                                                                                                                                                                     ********             ..       0      31 I 31       RCORDR 50050     1     0 EFFLUENT GROSS VALUE 0                           PERMIT REQUIREMENT
                                                                            ********                REPORT DAILY MX MGD                ********          ********            ********            -               CONTIN uous RCORDR NAME/TITLE PRINCIPAL EXECUTIVE OFFICER           1 Certify undef penally of law lhallhi' document and all aua<:tmcnts WUnl prepared under my                                                          TELEPHONE                    DATE dorectJon or supeMsoon in occonl...-.:e wo\h e system deso~ned to assuro that qualified Jl<lf$0MBI t?-

Michael D . Skaggs . prcp<irly galh!!r and evaluate the tnfonnatlon submilled. Based on my inquiry of the~ or persons who manage the system, or Ihose persoos dorectly responsoble for gathering "'" Sequoyah Site esident 423 Information, lhe informaiJon submllled IS

  • lo lhe best of my knowledge and belief. true, aCOJl1lle, 843-7001 11 01 06 Sequoyah Site Vice President and complete. I am aware lhal \herll are significant penalties IO< 5Ubmilting false tnformalton. SIGNATURE OF PRINCIPAL EXECUTIVE including \he possibiloty or fine and lmprosoomenl for knowing violations_

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED- - -- - COOL COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No closed mode operation. The following information is included in an attachment: 1. CCW data 2. veliger monitoring data EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2

DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons AnaJysls Date/Time Analyst Method No water would come out of the pump. No sample could be obtained. CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 11/09/2010@ 1030 <0.10 mg/1 11/1212010@ 0403 KLM EPH

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Temp. ("C) Sample Date Asiatic LOCATION LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp. ("C) Clams/m3 Clam 11/03/2009 133 0 16 11/03/2009 76 16 In plant RCW CMW 11/10/2009 417 6.1 16 11/10/2009 25 16 In plant RCW CMW 11/17/2009 269 0 16 11/17/2009 0 16 In plant RCW CMW 11/24/2009 36 50 15 11/24/2009 18 15 In plant RCW CMW 12/01/2009 32 0 13.5 12/01/2009 0 13.5 In plant RCW WE 12/08/2009 38 0 11 12/08/2009 0 11 In plant RCW CMW 01/05/2010 0 0 6 01/05/2010 0 6 In plant RCW B 01/12/2010 0 0 5 01/12/2010 0 5 In plant RCW 01/19/2010 0 0 6 01/19/2010 0 6 In plant RCW p 01/26/2010 32 0 7.5 01/26/2009 0 7.5 In plant RCW NRT 02/02/2010 0 0 7 02/02/2010 0 7 In plant RCW MSWIWDT 02/09/2010 0 0 8 02/09/2010 0 8 In plant RCW BL/TC 02/16/2010 0 0 5 02/16/2010 0 5 In plant RCW BJ 02/23/2010 11.7 0 7 02/23/2010 0 7 lnplant RCW BJ 03/02/2010 0 0 6 03/02/2010 0 6 In plant RCW PB 03/09/2010 0 0 8 03/09/2010 0 8 In plant RCW MJW 03/16/2010 0 0 10 03/16/2010 0 10 In plant RCW BC 03/23/2010 14 0 11 03/23/2010 0 11 lnplant RCW BC 03/30/2010 0 0 14 03/30/2010 0 14 In plant RCW BAPO Apr-2010 no samples collected May-2010 no samples collected Jun-2010 no samples collected Jul-2010 no samples collected Aug-2010 no samples collected Sep-2010 no samples collected Oct-2010 no samples collected Nov-2010 no samples collected 12/07/2010 6 100 23 12/07/2010 0 23 Turbine PB 12/14/2010 0 0 10 12/14/2010 0 10 Turbine 12/22/2010 0 0 10.5 12/22/2010 0 10.5 RCW

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR} Nam~-~~SEOUOY~~UC~ARP~~ -- - OMB No. 2040-0004 Address _p.o._.e._ox_2ooo___ ---~INTER~FICUB-2A*S~L ______ _ F=- - - - TN0026450 ~ I - - -- 101 G ~ (SUBR 01) F- FINAL ---~OD~~AI~TN3~---- - --- rt PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE FaQ!jty_ _lVA..:_SEOUO'ffili.N!J.CLEA.R.eJ,ANT_ _ _ _ _ EFFLUENT Locatio_~MIL~NCN~- --------- ATTN: stephanie A. Howard From I,10... I 12 r::rbG MO 01 To 10 MO 12 IPAY I 31

                                                                                                                                                                              ***  NO DISCHARGE          D ...

NOTE: Read instructions before completinq this form C>< PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF ~PE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** 0.015 0.036 14 I 31 GRAB MEASUREMENT ** 19 0 50060 1 0 0 PERMIT ******** ********

                                                                                                                               .......           ********            0.10             0.10             MG/L               WEEK-        CALCTD REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                                               MOAVG           INSTMAX                                  DAYS TEMPERATURE- C, RATE OF                             SAMPLE                    ********                       1                                    ********          ********                                               31 I 31      CALCTD MEASUREMENT                                                                     62                                                                       **        0 CHANGE 82234      1     0 EFFLUENT GROSS VALUE 0                              PERMIT REQUIREMENT DAILYMX 2                 DEG C/HR
                                                                                                                                                 ********          ********          *******             --              CONTIN uous CALCTD SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE                                                                                                                             .

MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

                                                                                                                                                                     /'I (i"::;re*~~"'

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law thallhis clocumenl and all altadlments were prepared under my TELEPHONE DATE d"edion or supervosion on occordance wtlh a system designed lo assure lhal qualified personnel Michael D. Skaggs properly gather and evaluele lha inlormatoon submilted Based on my inquiry of tho parson or persons who manage lhe system. or those persons directly responstble for galhenng the Information. lhe information sulxmlled is , to lhe best or my knowledge and belief, ln.oe, aq:urate, 423 843-7001 11 01 06 Sequoyah Site Vice President and comple te I am aware that lhere ere signific:at11 penaltle* for submiUmg false lnfonnatlon. SIGNATURE OF PRINCIPAL EXECUTIVE I 1ncludong tho possd>ilily of fine and imprisonment for knowing violations_

               ~PED   OR PRINTED                                        -                  - -                - - -

OFFICER OR AUTHORIZED AGENT

                                                                                                                                                                                                  ~~~~-~MBER _              YEAR MO   DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                     (Reference all attachments here The only 8/CTP chemicial injection this period was Towerbrom 960.

EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facility NameA..ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) mw__~~SEOUOYA~UCLEARP~~ -- - (SUBR 01) OMB No. 2040-0004 Adtt[ess J..Q.JtOX 2QO(L _ _ _ _ _ _ _ _ _ _ _ _ _ _ _j!N_T.EBQEFICU!i:ZA-S~L _ _ _ _ _ _ _ F----:rNo6264so -ll- -1o1a1 F- FINAL ---~ID~~~~~---- ---- Fa~~-~~W~~~~~~PLANL ___ _ PERMIT NUMBER l DISCHARGE NUMBERl DIFFUSER DISCHARGE I"""I r~***c Ft I I EFFLUENT ~catioJAMI~~- - --- -- --- ATTN: stephanie A. Howard From 10 MD 10 01 To 10 MO 12 DAY 31

                                                                                                                                                                            *** NO DISCHARGE            D       ***

NOTE:: Read instructions before completina this form. X PARAMETER QUANTJTY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS BORON, TOTAL SAMPLE MEASUREMENT

                                                                        '********                 ********                  ..                                   <0.20                                  19         0       1/92 01022      1    0   0                           PERMIT                   ********                  ********                 ****               *******          REPORT             *******             MG/L                 QTRLY        GRAB REQUIREMENT EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
                                                                                --                                   L__               - - -          -                                           ~                 -
                                                                                                                                                        ~:.~,:.as;,.,,,.m NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cett1fy under penalty of law that this doct.lment and all atladvnents were prepared under my                                                                TELEPHONE                   DATE        I d1111dlon or supervision In acco<dance with a system designed to assure that qualified personnel Michael D. Skaggs                  proporly gather and evaluaiB th" information submitted Based on rny 1nqu1ry of the person or                                                                                                   I persons who menage lhe system. or those persons doreclly responsible for gathering the Information. tha lnlonnalJOn submiUed IS . to tha best of my knowledge and belief. trve, accurate,                                                423 I     843-7001         11      01     06 Sequoyah Site Vice President             and complete I am aware that !hare are signoficant penalties for sl.tlrntlting false fnformalJOn,       SIGNATURE OF PRINCIPAL EXECUTIVE                                                       I lnctudong the possoboloty of fone and irnpnsonment for knowing violations TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT
                                                                                                                                                                                                 ~~~~ I      NUMBER         YEAR     MO    DAY I COMMENTS AND EXPLANATION OF ANY VIOLATIONS               fHeference all attachments here Boron was sampled on 10/6/2010.

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES} Form Approved. MAJOR mw__N~SEOUO~~UCLEARP~~--- DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 Mmess___Y..O.l!_OX2J!O<L _ _ _ _ _ _ _ _ _ _ _ ---~INTER~~UB-2A-SQ~-- --- -- ---~OD~~AI~TN~~--- - ---- 1- ~~~~;] IDISCHARG:~~M:ER I F- FINAL rt I BIOMONlTORING FOR OUTFALL 101 ~clii~_~A~~~~~~~R~N~ --- I,.** I MO r%Wj***G EFFLUENT Loca~~AMU~~N~- - -- -- -- -- ATIN: stephanie A. Howard From 10 12 01 To 10 MO 12 OAY 31 I *** NO DISCHARGE D ... NOTE: Read instructions before completinQ this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE ANALYSIS MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA SAMPLE MEASUREMENT

                                                                          ..........                                          **          Monitoring             .......***

23 JRP3B 1 0 0 PERMIT ******** *******'* *- Not Required 45.2 .......... **-****** PERCENT SEE COMPOS EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES REQUIREMENT SAMPLE MEASUREMENT MINIMUM Monitoring

                                                                                                                                                                 -                  ....,.,.,.,.         23 PERMIT irRP6C     1    0   0                            PERMIT REQUIREMENT Not Required 45.2             ***'****'*         *'*******        PERCENT                 SEE      COMPOS EFFLUENT GROSS VALUE                                                                                                                      MIMINUM                                                                         PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMF'LE MEASUREM ENT PERMIT REQUIREMENT SAMF'LE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Michael D. Skaggs 1 Certify under penally ollaw \hatlhls document and all altachmen ts -re pr~ lJOde( my lrecl10n or supo!Vrs*on in aocordance With a system designed to assure lha.t quahf!Gd personnel prnpel1y galhef and evaluato the *nlormabon submitted Based on my Inquiry ol the person or parsons who manage lhe system, or lhose po180<1S directly responsible l or gathering lhe
                                                                                                                                                        \- \ /r~*

SeQuovah Site resident I TELEPHONE I DATE Sequoyah Site Vice President inlonnabon. lhe informalloo !lUbmitled ** . lo lhe best ol my knowledge and belief, lruu. accurate, and canplete. I am aware thallhere are s.l gnificant penalties lor submitbng false lnfOITTiallon. 1-- - - - -- - - - - I 423 843-7001 I 11 I 01 1 o6 SIGNATURE OF PRINCIPAL EXECUTIVE 1--- - -- - - -- -- - -- - -- - --l'noluding the possibility ol fine anollmprisonment for knowing violations.

             ~PED   OR PRINTED OFFICER OR AUTHORIZED AGENT           I AREA I    NUMBER        I YEAR I MO I DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                 fReference all attachments here Toxicity was not sampled in December 2010.

EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ Form Approved . MAJOR DISCHARGE MONITORING REPORT (OMR) mm~-~~SEOUOYA~UCLEARP~~--- (SUBR 01) OMB No. 2040-0004 &fdre~ __p.O.JtOX2000 ___________ _ ---~INmOF~ESB-2A-~L -- -- --- ---~ODDY~~Y.W2NL ~clii~_WA~~~YA~UC~RPLAN~ - -- __ _ ___ _ [ -:TR:~: e~~ 5

                                                                                                                                         .J I    :CH:G~~M!R J F- FINAL LOW VOL. WASTE TREATMENT POND To r~l EFFLUENT

~c&~~AMI~O~N~- -- -- -- --- ATIN: stephanie A. Howard From Iv***i 10 MO 12 r:?T'"G 01 10 MO 12 I OAY 31 I *** NO DISCHARGE D ... NOTE: Read instructions before completinQ this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE UNITS ANALYSIS PH SAMPLE MEASUREMENT

                                                                          ........                 MAXIMUM UNITS MINIMUM 7.3 AVERAGE MAXIMUM 7.8                12       0      14 I 31       GRAB 00400     1    0    0                           PERMIT                     ********                  "'*******                                  6.0               ********               9.0                su              THREE/        GRAB REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                      MINIMUM                                   MAXIMUM                                  WEEK SOLIDS, TOTAL SUSPENDED                        SAMPLE                           55                       94                                  ********                 6                     9                        0       5 I 31       GRAB MEASUREMENT                                                                     26                                                                              19 00530     1    0    0                           PERMIT REQUIREMENT 380                       1250              LBS/DY              ********                30                  100               MG/L            WEEKLY         GRAB EFFLUENT GROSS VALUE                                                    MOAVG                     DAILYMX                                                         MOAVG             DAILY MX OIL AND GREASE                                  SAMPLE                       <55                        <61                 26               ********                <6                   <6                19       0        5 I 31      GRAB MEASUREMENT 00556     1    0    0                           PERMIT REQUIREMENT 190                        250              LBS/DY              ********                15                   20               MG/L            WEEKLY         GRAB EFFLUENT GROSS VALUE                                                    MOAVG                     DAILYMX                                                        _MO AVr;_          DAILY MX FLOW, IN CONDUIT OR THRU                        SAMPLE                      1.116                      1.337                03               ********              ********            ********             **        0      31 I 31     TOTALZ MEASUREMENT TREATMENT PLANT 50050    1    0    0                           PERMIT REQUIREMENT REPORT                     REPORT                 MGD                ******"'*             ********            ********
                                                                                                                                                                                                            ..                SEE       TOTALZ EFFLUENT GROSS VALUE                                                    MOAVG                    DAILYMX                                                                                                                   PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
                                                                                                                                                       '1:-tf. ~Pre,;deot NAME/TITLE PRINCIPAL EXECUTIVE OFFICER        I Certify undor penally or law thai lhls dorumonl and all allachments were prepared under my                                                              TELEPHONE                   DATE direction or supe<Vislon in accordance with a system <fes19ned to assure thai qualified personnel Michael D. Skaggs                   proper1y gather and 8'1Siusla lhe Information submitted Based on my Inquiry of the person or persons who manage lhe sysll!fl1. or tho!.EI persons directly responsible for galharlng lhe lnl011t1aUon, the Information submolled os
  • to the best al my knowledge and belle!, true. accurate. 423 843-7001 11 01 06 Sequoyah Site Vice President and rornplate. I am aware thai there are SiiJildlcanl penaiOes for submilting false lnformalion, SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED Including the pos51bihly of fine end lmprlsonmenl for knowing 11101a11ons OFFICER OR AUTHORIZED AGENT AREA COnE_L_

I NUMBER YEAR MO DAY _ I

                                         -              - --    ** -    -    -         -   -   -       - --    -    * - -- - -                                             -             -   - -                                 -~

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility NameA-ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMRI Nam~-N~SEOUOYA~UCLE~P~~- - - (SUBR 01) OMB No 2040-0004 _Mdress _p..Q .~OX2.QOCL _ _ _ _ _ _ _ _ _ _ _ - --~INTEROF~ESB-2A-SQ~ - ---- -- I -- TN0026450 - -- 1~ - -107 G~ F- FINAL. - --~@DY~AISY.TN~38L _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBE METAL CLEANING WASTE POND Facili~-m~~~YA~~LE~PLANL _ _ _ _ ~~io~AMI~~~NIT_ _ _ _ _ _ _ _ _ ATTN: stephanie A. Howard From 1 , ... I MD 10 12 r9** o Fi 01 To 10 MO 12 1 PAY 1 31 EFFLUENT

                                                                                                                                                                               *** NO DISCHARGE           IXX I ***

NOTE: Read instructions before completinSI this form X PARAMETER OUANTrrY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS I PH SAMPLE ******** '* ******* ******** MEASUREMENT 12 00400 1 0 0 PERMIT REQUIREMENT

                                                                           ********                  ********                                   6.0                ********             9.0               su                DAILY          GRAB EFFLUENT GROSS VALUE
                                                                                                      ...........                          MINIMUM                                 MAXIMUM SOLIDS, TOTAL SUSPENDED 1

SAMPLE MEASUREMENT PERMIT

                                                                                                                               -             ********              ********                               19 MG/L 00530            0  0 REQUIREMENT
                                                                           ********                   ********               ****            ********              ********             30                                  DAILY       COMPOS EFFLUENT GROSS VALUE                                                                                                                                                                DAILYMX OIL AND GREASE                                    SAMPLE MEASUREMENT PERMIT
                                                                                                                                             ********              ********                               19 MG/L               DAILY 00556     1      0  0 REQUIREMENT
                                                                           ********                   ********                               ********              ********             15                                                 GRAB EFFLUENT GROSS VALUE PHOSPHORUS, TOTAL (AS P)                          SAMPLE MEASUREMENT
                                                                                                                               -              ********             ........        DAILY MX 19 00665     1      0   0                             PERMIT                   *******"'                  ********               ****            ********              ********             1.0              MG/L               DAILY       COMPOS REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                                                                DAILYMX COPPER, TOTAL (AS CU)                             SAMPLE                    ********                   ********                                                     ********

MEASUREMENT 19 01042 1 0 0 PERMIT ******** ******** ******** ******"* 1.0 MG/L DAILY COMPOS REQUIREMENT EFFLUENT GROSS VALUE IRON, TOTAL (AS FE) SAMPLE MEASUREMENT DAILYMX 19 01045 1 0 0 PERMIT REQUIREMENT

                                                                                                                             ....             ********             ********             1.0              MG/L               DAILY        COMPOS EFFLUENT GROSS VALUE                                                                                                                                                                DAILYMX FLOW, IN CONDUIT OR THRU                         SAMPLE                                                                                       ********             ********           ********

MEASUREMENT 03 ** lfREATMENT PLANT

!i0050    1     0   0                             PERMIT                 REPORT                    REPORT                    MGD              ********              ********          ********
                                                                                                                                                                                                          ....              DAILY        CALCTD REQUIREMENT EFFLUENT GROSS VALUE
            --              --     ~ ~ -          ----                    MOAYJJ                  DAILY MX r:1:.£~.~*"'

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law lhallhis document and all allachmenls were prepared under my TELEPHONE DATE Clirection or superv1s1on 1n accordance with a system designed lo assure !hal qualified personnel Michael D. Skaggs properly gather and evaluate lhe information submitted Based on my inquiry of the person or persons who manage the system, or those persons direclly responsible for galhering the Information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 11 01 06 Sequoyah Site Vice President end complete. 1 am aware that there are sign1f1cant penalties for submitting false information, I SIGNATURE OF PRINCIPAL EXECUTIVE Including the possibility of fine and imprisonment for knowing viola lions ITPED OR PRINTED OFFICER OR AUTHORIZED AGENT

                                                                                                                                                                                                   ~~~ I       NUMBER YEAR       MO    DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                  (Reference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3/99)             Previous editions may be used                                                                                                                                                     Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) NamL _~~SEOUOYA~UCLEARP~~--- (SUBR 01~ OMB No. 2040-0004 Address __p_,Q.JtOX.200Q_ _ _ _ _ _ _ _ _ _ _ _ _ __ _ (INTERQEI:!CU~A*SONL - --~ODDY~~Y.TN~3BL _ _ _ _ _ _ _

                                                                                                    \~~=::.                               J lDIS:RG~M~:J                   F- FINAL RECYCLED COOLING WATER Ix*** i r9RING Fl                                               I Faclli~_NA~~~YA~~LE~PLANL _ _ _ _

Locatio~AMirrONCOUNIT_ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN: stephanie A. Howard From 10 MQ 12 01 To 10 MQ 12 I 31 PAY *** NO DISCHARGE IXX I *** NOTE: Read instructions before completinA this form. PARAMETER TEMPERATURE, WATER DEG. CENTIGRADE z 0 0

                                         ><    SAMPLE MEASUREMENT AVERAGE QUANTITY OR LOADING MAXIMUM UNITS 04 MINIMUM QUALITY OR CONCENTRATION AVERAGE MAXIMUM             UNITS 04 DEGC NO.

EX FREQUENCY OF ANALYSIS SAMPLE TYPE I 00010 PERMIT REQUIREMENT

                                                                         "*******                                        DEGC               ********            ********            38.3                                  DAILY       GRAB-4 INSTREAM MONITORING PH                                              SAMPLE MEASUREMENT DAILYMX 12 00400      1     0   0                           PERMIT 6.0               ********            9.0                su              WEEKLY         GRAB REQUIREMENT EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED                         SAMPLE MEASUREMENT
                                                                          ********                   ********               ..            MINIMUM
                                                                                                                                            *******ill           ********

MAXIMUM 19 00530 1 0 0 PERMIT ******** ******** **** ******** ******** 30 MG/L DAILY COMPOS REQUIREMENT EFFLUENT GROSS VALUE OIL AND GREASE SAMPLE MEASUREMENT DAILY MX 19 00556 1 0 0 PERMIT ******** ******** **** ******** ****"*** 15 MG/L DAILY GRAB REQUIREMENT EFFLUENT GROSS VALUE DAILYMX FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** MEASUREMENT 03 ** TREATMENT PLANT 50050 1 0 0 PERMIT REPORT REPORT MGD ******** ***"**** ******** ..... DAILY CALCTD REQUIREMENT EFFLUENT GROSS VALUE MO_AVO DAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 19 50060 1 0 0 PERMIT REQUIREMENT

                                                                          ********                   ********              ....              ********            ********            0.10              MGil             WEEKLY         GRAB-4 EFFLUENT GROSS VALUE                                                                                                                                                             DAILYMX SAMPLE MEASUREMENT PERMIT REQUIREMENT r:JS,~.:~.*.,

NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law lhallhis document and all attachments were prepared under my TELEPHONE DATE directlon or superv1sion 1n accordance wilh a system designed to assure thai qualified personnel Michael D. Skaggs properly gather and evaluate the mformation submitted Based on my inquiry of lhe person or persons who manage the system. or those persons directly responsible for gathering the tllformalion, the information submined is , to the best of my knowledge and belief, true, accurale. 423 843-7001 11 01 06 Sequoyah Site Vice President Elnd complete I am aware lhat there are significanl penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations SIGNATURE OF PRINCIPAL EXECUTIVE I ITPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY rooF -- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAMEJADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) m~- -~~SEOUOYA~UCLEARP~~ --- (SUBR 01) OMB No. 2040-0004 Mdress _p...Q.JtO.X.2.000_ _ _ _ _ _ _ _ _ _ _ _

                                                                                                     ~~~~! a~                            J 1 DISCHARG: ~~M:EJ

---~INTER~~~B-2A-SQ~ ------- 5 F- FINAL ---~DY~~Y.TN23BL __ ___ _ _ 1 RECYCLED COOLING WATER ~~~ -~A~~~YA~~~RPLANL _ _ _ _ t~TriNG p~i MO DAY 1 ~c~~~AMIU~~NTI_ _ _____ __ _ EFFLUENT 1 YEAR IXX I *** ATIN: stephanie A. Howard From 10 ! 12 MO 01 To  ! 10 12 I 31 *** NO DISCHARGE NOTE: Read instructions before completinQitlis form. PARAMETER IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 0

                                         ><    SAMPLE MEASUREMENT PERMIT AVERAGE OUANTITY OR LOADING MAXIMUM UNITS MINIMUM 45.2 OUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS 23 PERCENT NO.

EX FREQUENCY OF ANALYSIS SEMI SAMPLE TIPE COMPOS EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES REQUIREMENT SAMPLE MEASUREMENT MINIMUM

                                                                                                                                                                  ********            ********             23 ANNUAL TRP6C     1    0      0                          PERMIT REQUIREMENT
                                                                         ********                 ********               **"'*               45.2                 ********            ********         PERCENT              SEMI        COMPOS EFFLUENT GROSS VALUE                                                                                                                     MINIMUM                                                                           ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT                                                                                                                                  .

SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE

                                                                                                                                                                            -~

direction or supervision in accordance with a system designed to assure that qualified personnel Michael D . Skaggs proper1y gather and evaluate the information subm~ted . Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the infomation. the infomation submitted is . to the best of my knowledge and belief. true, accurate. Sequoyah Site Vi. re dent

                                                                                                                                                                                   --                                                         06 Sequoyah Site Vice President              and complete I am aware that there are significant penalties for submitting false Information,        *-- * -
SI(jNATURt Ul- PRINCIPAL EXECUTIVE 1-- - - - - -- - -- - - -- - - - -- - - t lncluding the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS fReference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMmEE NAME/ADDRESS (Include Facility NameA.ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDE$) Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) NamL_N~SEOUOYA~UCLEARP~~ - -- (SUBR 01) OMB No. 2040-0004 _MQ[ess _p.O.JtOX 2J!OIL _ _ _ _ _ _ _ _ _ _ _ p;!~~~~~ a~R~ Lo:::d ~~M~ER I 5 ---~INTEROF~~~A*S~L _ _ _ _ _ _ _ ---~@~~M~W37~--- -- --- I F- FINAL BACKWASH Facili~-m~EQ~~~UCLEARP~N~ - -- - ~~io~AMI~O~NIT ATTN: stephanie A. Howard From 1 X... 10 I MQ 12 r:g*'~" 01 To rt10 MQ 12 1 DAy 31 1 EFFLUENT

                                                                                                                                                                           *** NO DISCHARGE             D     ***

NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE ******** ******** ******** ******** 0 0 1 I 31 VISUAL MEASUREMENT ** 9A 01345 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT

                                                                          ********                 ********                 *-*             ********            ********        REPORT MOTOTAL PASS=O FAIL=1 SEE PERMIT VISUAL OIL AND GREASE VISUAL                           SAMPLE                      ****'****                     0                                   ********           ********         ********                               1 I 31      VISUAL MEASUREMENT                                                                      94                                                                         **       0 84066     1    0 EFFLUENT GROSS VALUE 0                            PERMIT REQUIREMENT
                                                                          ********               REPORT MOTOTAL YES=1 NO=O
                                                                                                                                            ********            ********         ********               -                SEE PERMIT VISUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT (L~~;~em NAME/TinE PRINCIPAL EXECUTIVE OFFICER          I Certify under penalty of law that this document and all attachments were prepared under my                                                          TELEPHONE                  DATE
                                                ~~rectlon or supervision In accordance with a system designed to assure lhal qualified personnel Michael D . Skaggs                   JProperly gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the Sequoyah Site Vice President information, the information submined is
  • to the best of my knowledge and belief. true. accurate. 423 I 843-7001 11 01 06 ana complete. I am aware that there are significant penalties lor submitting false information. SIGNATURE OF PRINCIPAL EXECUTIVE TIPED OR PRINTED including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA ('Of""'= I NUMBER YEAR MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS tlleference all attachments here Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES} MAJOR Form Approved . DISCHARGE MONITORING REPORT (OMRJ m~--~~SEOU~A~OCLEAAP~ --- (SUBR 01) OMB No. 2040-0004 Address __p .O.JtOX~ - _____ _____ _ p~~~~~~~SB~R ~~M~ER l ---~1Nm~~~B-2A*S~L _ _ _ _ _ _ _ 1 F- FINAL - --~DY~AISY.rn2NL _ _ _ _ _ _ _ [ ll::HARGE BACKWASH I,,.. I rq***GF! IDAY I ~cili~_WA~EQ~YA~~~~P~NL _ _ _ _ ~~io_~~m~NTI_ ______ __ _ EFFLUENT From 10 MO 12 01 To 10 MQ 12 31

                                                                                                                                                                                *** NO DISCHARGE            D ...

ATIN : Stephanie A. Howard NOTE: Read instructions before completin!l this form. X PARAMETER OUANTrrY OR LOADING OUALrrY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY} SAMPLE MEASUREMENT --******* ******** ... ******** 0 9A 0 1 I 31 VISUAL 01345 1 0 0 PERMIT ******** ******** **** ******** ******** REPORT PASS=O SEE VISUAL REQUIREMENT FAIL=1 EFFLUENT GROSS VALUE MOTOTAL PERMIT OIL AND GREASE VISUAL SAMPLE MEASUREMENT

                                                                          ********                      0                   94              ********              ********            ********              ..       0      1 I 31          VISUAL 84066     1     0   0                            PERMIT                    ********                REPORT                 YES=1              ********              ********            ********             ....               SEE           VISUAL REQUIREMENT                                                                   NO=O EFFLUENT GROSS VALUE                                                                              MOTOTAL                                                                                                                   PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER          1Certify under penally of law lhallhis documenl and all attachmenls were prepared under my                                                               TELEPHONE                    DATE dlreci!On or supervisiOn In accordance wi!h a sys1em des*gned to assure thai qualified personnel Michael D. Skaggs                   properly galher and evaluale the informalion submitted Based on my inquiry of lhe person or persons who manage lhe syslem. or lhose persons directly responsible for galhering !he informalion. lhe informalion submitted is
  • lo the best of my knowledge and belief. lrue. accurale. 1 - -- - - - - - - - - - - - - - - - - - - 1 423 843-7001 11 01 06 Sequoyah Site Vice President and complele I am aware lhallhere are significanl penallies for submitling false information. SIGNATURE OF PRINCIPAL EXECUTIVE 1 - - - - - -- - - - - - - - -- - - - - - - - - i lncluding the possibilily of fine and imprisonment for knowing viola lions OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TIPED OR PRINTED COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here Operations performs visual inspections for floating debris and oil and grease during all backwashes.

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Fonn Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) ~m~-N~SEOUOY~~UCLEARP~~ --- (SUBR 01) OMB No. 2040-0004 _Mdress _p_.Q.JtOX2_QOQ_ _ _ _ _ _ _ _ _ _ _ _ [-~~=-~ IDISCHARG; ~~M~ER I F- FINAL - --~INTER~FICUB-2A-S0~ -- ---- ___ 3@M~~~m2~- - --- --- WASTEWATER & STORM WATER ~~~-~A~~~~~~~RPLANT _ _ __ _ f:?1"'"" Fl I EFFLUENT ~cat~~A~N~~- - -- -- - --- ATTN: Stephanie A. Howard From I""I 10 MO 12 01 To 10 M 12O DAY 31 I *** NO DISCHARGE IXX I ... NOTE: Read instructions before completinq this form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED 00300 1 0 0 (DO) SAMPLE MEASUREMENT PERMIT 19 REQUIREMENT

                                                                            ********                  ********                                   2.0                 ********                              MG/L              TWICE/         GRAB EFFLUENT GROSS VALUE                                                                                                                        DAILYMN                                                                             WEEK SOLIDS, TOTAL SUSPENDED 00530      1    0     0 SAMPLE MEASUREMENT PERMIT
                                                                                                                                                                      ********                               19 MG/L REQUIREMENT
                                                                             ********                 ********                  ****                                  ********             100                               TWICE/         GRAB EFFLUENT GROSS VALUE                                                                                                                                                                   DAILYMX                                  WEEK SOLIDS, SETTLEABLE                                SAMPLE MEASUREMENT
                                                                                                                                 -            ********                ********                               25 00545      1    0 EFFLUENT GROSS VALUE 0                            PERMIT REQUIREMENT
                                                                             ********                 ********                  -**           ********                ********             1.0 DAILYMX MUL               ONCE!

MONTH GRAB FLOW, IN CONDUIT OR THRU SAMPLE MEASUREMENT 03 TREATMENT PLANT 50050 1 0 0 PERMIT REQUIREMENT REPORT REPORT MGD ******** ******** ******** . ONCE/ ESTIMA EFFLUENT GROSS VALUE MOAVO DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

                                                                                                                                                                 .....    /
                                                                                                                                                            ~..L?~.. ~,;,~"'

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law thallhls document lm(j all altachmenls were prepared lmder my TELEPHONE DATE d*red00'1 or suporvlslorl l<1 accordance wrlh a system destgned to assure thai qualified personnel Michael D. Skaggs properly gather and IM!Iuate the lnformalloo sulxlulled Based on my Inquiry of 1he ~nor persons who manage !he syslem, 0< lhasa persons dlredly raspoosrble IO< galherrng the lmoonation, lhe Information submrHed Is . to the oost of my knowledge and oollef. true. accurale, 423 843-7001 11 01 06 Sequoyah Site Vice President and complete I am aware lhallhere are signrlicant penal119s le< sU>mithng false informatoon, SIGNATURE OF PRINCIPAL EXECUTIVE I i(lClud1ng lha possrblllty of fine and impnsonment for ilnoWtng YIOIBUOflll.

                                                                                                                                                                                                      ~~~~ I OFFICER OR AUTHORIZED AGENT                           NUMBER        YEAR      MO    DAY TYPED OR PRINTED                                                                                                                                                                                                                    -

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (f?eference all attachments here During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- December 2010 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 01/06/2011 CONCURRENCES Name R c Signature - Comment Date v N B.M. Love X S. A. Howard X D.A. Day X M.D. Skaggs X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.}}