Latest revision as of 19:04, 25 February 2020

Text

Annual Water Withdrawal Updates for 2008
	ML13289A205
Person / Time
Site:	Sequoyah
Issue date:	08/14/2008
From:	Howard S; Tennessee Valley Authority
To:	; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
Shared Package
ML13289A109	List: ML13289A092; ML13289A094; ML13289A095; ML13289A096; ML13289A097; ML13289A098; ML13289A099; ML13289A100; ML13289A101; ML13289A108; ML13289A111; ML13289A112; ML13289A113; ML13289A114; ML13289A115; ML13289A116; ML13289A117; ML13289A118; ML13289A119; ML13289A120; ML13289A121; ML13289A122; ML13289A123; ML13289A124; ML13289A125; ML13289A126; ML13289A127; ML13289A128; ML13289A129; ML13289A130; ML13289A132; ML13289A133; ML13289A134; ML13289A136; ML13289A137; ML13289A153; ML13289A158; ML13289A164; ML13289A165; ML13289A166; ML13289A167; ML13289A168; ML13289A169; ML13289A170; ML13289A171; ML13289A172; ML13289A173; ML13289A174; ML13289A197; ML13289A198; ... further results
References
	Download: ML13289A205 (187)
	v • d • e

{{#Wiki_filter:S58 080814 800- NPDES CORRESPONDENCE August14,2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JULY 2008 Enclosed is the July 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please coritact me at (423) 843-6700 if you have any questions or comments. Sincerely, ,j 1 Jt~1GtJuGc (}~I),LX(J- d Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN J. D. Smith, OPS 4A-SQN T. P. Cleary, OPS 4A-SQN G. R. Signer, WT 6A-K D. E. Pittman, BR 4T-C EDMS, WT CA-K (Enclosure) DMR0807.doc

Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 August 14, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JULY 2008 Enclosed is the July 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, ~~- . )-hoL<1A'--"- 0 JJotUc&c d Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Pnnted on recycled paper

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~~NT _ _ _ _ DISCHARGE MONITORING REPORT (OMRI OMB No. 2040-0004 (SUBR 01) AddresE__E~BOX2000 - - - - - - - - - - - - ---~TEROFFICESB-~---------- TN0026450 101 G F- FINAL ---~DDY-DAISY_~N3738L ______ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facility_ .JYA -_§f:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio_!L __tjAMIL TOli_COUNTY_ _ _ _ _ _ _ _ _ _ _ EFFLUENT DAY From NO DISCHARGE [J *** ATTN: Stephanie A. Howard 31 NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION I NO. IFREQUENCY/ SAMPLE EX OF TYPE WATERDEG. 0 WATERDEG. 0 0 0 j~~Q~e..-td NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 08 08 14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE

                                                   ------                                                                                                                                                                      L__       ---

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2

DMR Attachment CCW Data

CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 07/09/2008 @ 1200 0.24 mg/1 07/11/2008 @ 1033 CLS TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 07/09/2008 @ 1150 <0. 10 mg/1 07/11/2008 @ 1044 CLS TN EPA 8015

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRI OMB No. 2040-0004 (SUBR 01) Add reS§_ .£.~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 G F- FINAL ---~TEROFFICESB-~---------- ---~DDY-DAIS~~N3738L ______ _ DIFFUSER DISCHARGE Facility_ JYA -.§EQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.n_ _!:!AMILTOl:!..COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard From I YEAR os I -- I - . I DAY 31

                                                                                                                                                                             *** NO DISCHARGE           D        ***

NOTE: Read instructions before completin!l this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. EX IFREQUENCY OF SAMPLE TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS CHLORINE, TOTAL RESIDUAL ******** ******** ******** 0.019 0.038 0 29/31 GRAB 19 50060 0 0 EFFLUENT GROSS VALUE MPERATURE

C, RATE OF CHANGE 82234 1 0 0 FFLUENT GROSS VALUE W&~t(LB1.LLO. cilo~d0.LCI NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel I Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 08 08 14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY TYPED OR PRINTED CODE -- --

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured: 1. Biodetergent 73551 (max. calc. cone. was 0.018mg/L--Iimit 2.0mg/L) 2. MSW-101 (max. calc. cone. was 0.062mg/L-Iimit 0.2mg/L) 3. H-150M (max. calc. cone. was 0.038mg/L--Iimit 0.050mg/L) 4. H-150M (low detection level analytical method was <0.020mg/L--Iimit 0.050mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUO~~UCLEARP~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMR} OMB No. 2040-0004 (SUBR 01) Addres_L _E.~BOX 20QQ_ _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 T F- FINAL ---~TEROFFICESB-~---------- - _ _ _§.ODDY- DAISY_ _IN 37381._ _ _ _ _ _ _ _ BIOMONITORING FOR OUTFALL 101 Facility_ _IYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!]_ .JjAMILTOli.COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard From I YEAR os I -. I -

I DAY 31 NO DISCHARGE D ...

NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUENCY' SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM 0 0 EFFLUENT GROSS VALUE

                                                                                                                                                     ~lO.IL~ Cl~,dCt ~_J NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my                                                                 TELEPHONE                    DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary                  property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage lhe system, or those persons directly responsible for gathering the               Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true,                                                     423       843-6700        08      08     14 Site Vice President                 accurate, and complete. I am aware that there are significant penalties for submitting false       SIGNATURE OF PRINCIPAL EXECUTIVE                 I information, including the possibility of fine and imprisonment for knowing violations.              OFFICER OR AUTHORIZED AGENT             AREAl      NUMBER        YEAR      MO    DAY TYPED OR PRINTED                                                                                                                                                                 CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Toxicity was not sampled in July 2008. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Add res§_ ...f.Q.,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 103 G F- FINAL ---~TEROFFICESB-~---------- ---~DDY-DAIS~~N3738L ______ _ LOW VOL. WASTE TREATMENT POND Facilitv_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l_ _!:::!AMILTOJ::!.COUNTY __________ _ EFFLUENT YEAR DAY 31

                                                                                                                                                                               *** NO DISCHARGE           D ...

ATIN: Stephanie A. Howard From I 08 I -. I -

I NOTE: Read instructions before completinQ this form.

PARAMETER QUALITY OR CONCENTRATION I NO. IFREQUENCY I SAMPLE EX OF TYPE ANALYSIS 0 0 J~vLou~, TOTAL SUSPENDED 0 0 0 0 GROSS VALUE GROSS VALUE

                                                                                                                                                         ~~\a.Jt.uO.J1h~A d NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my                                                                     TELEPHONE                    DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary                   properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, Principal Environmental Engineer        423       843-6700         08      08     14 Site Vice President                  accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.

SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320*1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres,L_E~BOX2000 - - - - - - - - - - - - TN0026450 107 G F- FINAL ---~TEROFFICESB-~---------- _ _ _ ~ODDY- DAISY_ ____TN 3738i._ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER METAL CLEANING WASTE POND Facilitv_.JYA-_§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio..!l_ _!:!AMIL TO_!i_COUNTY_ _ _ _ _ _ _ _ _ _ _ EFFLUENT YEAR From NO DISCHARGE I XX I *** ATTN: Stephanie A Howard 08 NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM AVERAGE PH ******** ******** ******** 0 0 50050 1 0 0 EFFLUENT GROSS VALUE

                                                                                                                                                      ~t&JLu001'1CCu c NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my                                                                    TELEPHONE                    DATE     !

direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 I 843-6700 08 08 14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR _L_ MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMS No. 2040-0004 (SUBR 01) Addres.L_ __E.Q,_BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 110 G F- FINAL ---~TEROFFICESB-~---------- ---~DDY-DAISY_~N373BL ______ _ RECYCLED COOLING WATER FaciliN_~A-~QUO~HNUCL~RP~N~----- Locatio.!l._ .J::!AMILTOl:!_COUNTY__________ _ EFFLUENT Y~R ATTN: Stephanie A. Howard From I os I _. I -- I NO DISCHARGE IXX I *** NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUENCY/ SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM E MAXIMUM UNITS

                                                                               ********                    ********              04               ********           ********                              04 0     0 I;)ULiu;:.,    TOTAL SUSPENDED 0     0 1~nLV"'INI:.. TOTAL RESIDUAL 1     0     0 EFFLUENT GROSS VALUE
                                                                                                                                                        ~i1CLPL~o(.clh-L't'~*,cd NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my                                                                     TELEPHONE                    DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary                  property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true,                                                      423      843-6700         08      08     14 Site Vice President                 accurate, and complete. I am aware that there are significant penalties for submitting false        SIGNATURE OF PRINCIPAL EXECUTIVE                 I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing Violations.

OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Add res.§_ _f.~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 F- FINAL ---~TEROFFICESB-~---------- - _ _ _.§.ODDY- DAISY_ _.IN 3738£.. _ _ _ _ _ _ _ RECYCLED COOLING WATER Facility_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l._ .J::!AMILTO_!LCOUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard From __ I _. _

NO DISCHARGE IXX I ***

1 NOTE: Read instructions before completinq this form. PARAMETER QUALITY OR CONCENTRATION I NO. IFREQUENCY/ SAMPLE EX OF TYPE 7DAY CHR 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE Timothy P. Cleary direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or vu"--c( persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 08 08 14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Add res.§_ _E.~BOX_1900 ___________ _ TN0026450 116 G F- FINAL ---~TEROFFICESB-~---------- - _ _ _§.ODDY- DAISY_ __.IN 37381._ _ _ _ _ _ _ _ BACKWASH Facility_ ...JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l_ .J:!AMILTO.Ji.COUNTY__________ _ EFFLUENT YEAR DAY NO DISCHARGE D *** ATTN: Stephanie A. Howard From I oa I -- I - - I 31 NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUENCY I SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM

           , FLOATING (SEVERITY)                                             ********                   ********               ..               ********           ********             0                9A         0 AND GREASE VISUAL 0    0 EFFLUENT GROSS VALUE
                                                                                                                                                      ~'Cut~O~ib>~u CLZ c~

NAMEffiTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that lhis document and all attachments were prepared under my TELEPHONE DATE ! direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of lhe person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, lrue, 423 843-6700 08 08 14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT DAY AREAl NUMBER YEAR MO TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPD£S) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRI OMB No. 2040-0004 (SUBR 01) Addres_L _E.~BOX 20QQ_ _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 117 G F- FINAL ---~TEROFFICESB-~---------- - _ _ 20DDY- DAISY_ _IN 3738!_ _ _ _ _ _ _ _ BACKWASH Facilitv_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!L _!jAMILTOl:!...COUNTY __________ _ EFFLUENT From I YEAR os I -- I -- I NO DISCHARGE CJ *** ATTN: Stephanie A. Howard NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION J NO. JFREQ~ENCYJ SAMPLE TYPE UNITS MINIMUM AVERAGE MAXIMUM UNITS IUE:DI".I~. FLOATING (SEVERITY) .. ******** ******** 0 9A 0 0 0 0 ENT GROSS VALUE

                                                                                                                                                      ~cu~U~?~uc~~:d NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my                                                                    TELEPHONE                    DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary                    property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the                 Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true,                                                      423       843-6700        08       08     14 Site Vice President                   accurate, and complete. I am aware that there are significant penalties for submitting false        SIGNATURE OF PRINCIPAL EXECUTIVE                 I information, including the possibility of fine and imprisonment for knowing violations.               OFFICER OR AUTHORIZED AGENT             AREAl      NUMBER        YEAR      MO     DAY TYPED OR PRINTED                                                                                                                                                                    CODE_

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUO~~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Add res.§_ _E ~BOX 20QQ_ _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 118 G F- FINAL ---~TEROFFICESB-~---------- - _ _ _§.ODDY- DAISY_ _JN 37381.._ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER Facility_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!!_ .J::!AMILTO.!:!_COUNTY __________ _ MONITORING PERIOD EFFLUENT ATIN: Stephanie A. Howard From Y~R I ~~ ~A1Y I To I Y~R I ~7 I ~A1Y NO DISCHARGE I XX I *** NOTE: Read instructions before completin!l this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUENCY/ SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS (DO) ******** ******** ******** ******** 19 0 0 GROSS VALUE 50050 0 0 EFFLUENT GROSS VALUE

                                                                                                                                                        ~Yu~Ticil~v-1' A,~

NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 I 843-6700 08 08 14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

S58 080911 800 - NPDES CORRESPONDENCE September 11, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR AUGUST 2008 Enclosed is the August 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, \ 1

~;

~***:~}L~t\£ulA...<_.

      .4 Stephanie A. Howard
                      .       / *-* ** ( 1'1*

l/l >-- Q'lA.)cu. d Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN J.D. Smith, OPS 4A-SQN T. P. Cleary, OPS 4A-SQN G. R. Signer, WT 6A-K D. E. Pittman, BR 4T-C EDMS, WT CA-K (Enclosure) DMR0808.doc

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 September 11, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR AUGUST 2008 Enclosed is the August 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUO~~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres_L_.E~BOX2000 - - - - - - - - - - - - TN0026450 101 G F- FINAL ---~TEROFFICESB*~---------- _ _ _ _§_ODD.Y..: DAISY_ _IN 3738i._ _ _ _ _ _ _ _ PERMIT NUMBER DIFFUSER DISCHARGE Facility_ ...I:JA *...§!:OUOYAH NUCLEAR PLANT_ _ _ _ _ _..:. Locatio.!l_ .......!::!AMILTOH_COUNTY_ _ _ _ _ _ _ _ _ _ _ MQ..NITORING PERIOD EFFLUENT From Y~~R I ~~ I ~A1Y J To ~A1Y NO DISCHARGE D ... ATTN: Stephanie A. Howard NOTE Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION I NO. EX IFREQUENCY! OF SAMPLE TYPE ANALYSIS UNITS MINIMUM AVERAGE MAXIMUM UN

                                                                                                                                  ..               ********           ********           29.8              04         0       31 I 31   I MODELD WATER DEG.

0 0 FF. BETWEEN SAMP. & M DEG.C s 0 GRAB 50050 0 0 EFFLUENT GROSS VALUE

                                                                                                                                                          .J~~&-~d NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this document and all attachments were prepared under my                                                                      TELEPHONE                    DATE direction or supervision in accordance w1th a system designed to assure that qualified personnel Timothy P. Cleary                 properly gather and evaluate the information subm1tted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the                Principal Environmental Engineer informatton, the information submitted is , to the best of my knowledge and belief, true,                                                     423       843-6700         08       09     11 Site Vice President                 accurate, and complete. I am aware that there are significant penalttes for submttting false        SIGNATURE OF PRINCIPAL EXECUTIVE                I mformation, tncluding the possibility of fine and imprisonment for knowing vtolations                 OFFICER OR AUTHORIZED AGENT            AREAl      NUMBER         YEAR       MO    DAY TYPED OR PRINTED                                                                                                                                                                CODE                                     _L__

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3199) editions may be used Page 1 of 2

DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 08/20/2008 @ 1040 <0.10 mg/1 08/22/2008 @ 1551 JAB TN EPA 8015 CCW CHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 08/20/2008 @ 1035 <0.10 mg/1 08/22/2008 _@ 1603 JAB TN EPA 8015

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres_L _E Q._BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 G F- FINAL ---~TEROFFICESB-~---------- - _ _ _§.ODDY- DAISY_ _JN 3738L_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facility_ .-JYA- SEQUOYAH NUCLEAR PLANT _ _ _ _ _ _ Locatio.!)_ _!:!AMIL TO~COUNT!:_ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT YEAR I YEAR I MO I DAY NO DISCHARGE CJ *** ATTN: Stephanie A. Howard From I 08 To 08 08 31 NOTE Read instructions before completinq this form. PARAMETER NO. IFREQUE EX OF ANALYSIS MAXIMUM UNITS RINE, TOTAL RESIDUAL 0.034 19 0 0 0 GROSS VALUE N~~*~CUC NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEP'HONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or \ persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the *Information submitted is , to the best of my knowledge and belief, true, 423 843-6700 08 09 11 Site Vice President i accurate, and complete ..1am aware that there are significant penalties for submitting false I TYPED OR PRINTED information, including the possibility of fine and impnsonment for knowing violations SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE l NUMBER I YEAR MO L_____ D~ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured: 1. Biodetergent 73551 (max. calc. cone. was 0.017mg/L--Iimit 2.0mg/L) 2. MSW-1 01 (max. calc. cone. was 0.059mg/L--Iimit 0.2mg/L) 3. H-150M (max. calc. cone. was 0.039mg/L--Iimit 0.050mg/L) 4. H-150M (low detection level analytical method was <0 020mg/L--Iimit 0.050mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~VA-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres.§__E.!2.._BOX~OO - - - - - - - - - - - - ---~TEROFFICESB-~---------- TN0026450 101 T F- FINAL _ _ _ __§_ODD.Y_- DAISY_ _IN 3738L _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facility_ .3:/A -..§l:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio_Q_ .J:!AMIL TO_N_COUNTY._ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT ATTN: Stephanie A. Howard From YEAR 08 DAY I o:LJ To I YEAR I MO I 08 08 DAY 31 NO DISCHARGE [] ... NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM ITS STATRE 7DAY CHR ******** ******** ******** ******** 23 RIODAPHNIA 0 0 0 0 ENT GROSS VALUE

                                                                                                                                                 ~O:~cud NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my                                                             TELEPHONE                    DATE direction or supervision tn accordance with a system designed to assure that qualified personnel Timothy P. Cleary              properly gather and evaluate the information submitled. Based on my inqutry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer                                                   11 tnformation. the information submitted IS , to the best of my knowledge and belief, true,                                                      423       843-6700        08       09 Site Vice President             accurate, and complete. I am aware that there are s1gn1ficant penalties for submitting false        SIGNATURE OF PRINCIPAL EXECUTIVE                  I information, including the possibility of fine and imprisonment for know1ng VIolations.

TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT ~NUMBER YEAR MO DAY E -~- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in August 2008. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~VA-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) AddresL _.E.Q,_BOX_19QQ_ _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 103 G F- FINAL - _ _ 2.0DDY- DAISY _ _IN 373BL _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Facility_ _IYA -_§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio!l_ .J::!AMILTOl::!_COUNT:L_ _ _ _ _ _ _ _ _ _ _ MONITC RING PERIOD ! EFFLUENT ATIN: Stephanie A Howard From YEAR I MO o8 I o8 I 01 I DAY I YEAR I MO To I 08 I 08 I 31 1 I DAY NO DISCHARGE LJ *** NOTE: Read instructions before completinq this form PARAMETER QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM AVERAGE PH ******** ******** 7.5 ******** i;:>ULIU;:>, TOTAL SUSPENDED 0 0 NDUIT OR THRU PLANT 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1Certify under penalty of law that thts document and all attachments were prepared under my

                                                                                                                                                                   ~(};..

TELEPHONE DATE directton or supervision in accordance wtth a system destgned to assure that qualrfied personnel ---- Timothy P Cleary properly gather and evaluate the 1nformat1on submilled Based on my inquiry bf the person or persons who manage the system, or those persons dtrect!y responstble for gathenng the Principal Environmental Engineer 1nformalion, the information submilled is , to the best of my knowledge and belief, true, 423 843-6700 08 Site Vice President accurate, and complete. I am aware that there are sign.lficant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE mformation, 1ncluding the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres.L _f.Q,_BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 107 G F- FINAL ---~TEROFFICESB-~---------- - _ _ _§_ODD.Y_- DAISY_ _IN 3738L _ _ _ _ _ _ _ METAL CLEANING WASTE POND Facility_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l_ JjAMIL TOli_COUNTY __________ _ EFFLUENT YEAR DAY NO DISCHARGE I XX I *** ATTN: Stephanie A Howard From I os I -- I -

I 31 NOTE: Read instructions before completinq this form.

PARAMETER QUALITY OR CONCENTRATION SAMPLE TYPE AVERAGE PH ******** 0 0 r;:suLru;:s, TOTAL SUSPENDED 0 0 0 0 FFLUENT GROSS VALUE RON, TOTAL (AS FE)

        , IN CONDUIT OR THRU ENT PLANT 50050        1   0      0 EFFLUENT GROSS VALUE NAMErTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that thiS document and all attachments were prepared under my                                                         TELEPHONE                    DATE direction or supervi~ion in accordance with a system designed to assure that qualified personnel Timothy P. Cleary             properly gather and evaluate the information submitted. Based on my inquiry of the person or                                     Cv persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted IS, to the best of my knowledge and belief, true,                                                    423       843-6700        08      09     11 Site Vice President            accurate, and complete. I am aware that there are sign1f1cant penalties for submitting false     SIGNATURE OF PRINCIPAL EXECUTIVE                                                     -

information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _f.~BOX_19QQ_ _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 F- FINAL ---~TEROFFICESB-~---------- - _ _ _§.ODD.Y_* DAISY_ _IN 3738i_ _ _ _ _ _ _ _ PERMIT NUMBER RECYCLED COOLING WATER Facility_ .:JYA ._g:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!}_ _!jAMIL TOJ::LCOUNTY __________ _ EFFLUENT DAY NO DISCHARGE I XX I *** ATTN: Stephanie A Howard 31 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. )FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ERATURE, WATER DEG. ******** ******** 04 ******** ******** 04 ENTIGRADE z 0 0 INSTREAM MONITORING PH 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 0 0 NDUIT OR THRU PLANT 0 0 HLORINE, TOTAL RESIDUAL 50060 0 0 EFFLUENT GROSS VALUE

                                                                                                                                                ~twu.::.a..~A-C NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my                                                             TELEPHONE                    DATE I

direction or supervision in accordance with a system designed to assure that qualified personnel

',~

Timothy P. Cleary properly gather and evaluate the InformatiOn submitted. Based on my 1nquiry of the person or persons who manage the system, or those persons directly responsible for gathering the I Principal Environmental Engineer Site Vice President information, the information submitted IS, to the best of my knowledge and belief, true, 423 I 843-6700 08 09 accurate. and complete I am aware that there are significant penalties for submttting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED Information, including the possibility of f1ne and impnsonment for know1ng violations. _____L_ OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY i COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_2~-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Add res§_.£. ~BOX.1_900 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 110 T F- FINAL ---~TEROFFICESB-~---------- - _ _ _§.ODD.Y.: DAISY_ _JN 3738L _ _ _ _ _ _ _ RECYCLED COOLING WATER FaciliN_~A-SEQUO~HNUCLEARP~N~----- Locatio.!l.._ _!iAMIL TQ_!i_COUNTL _ _ _ _ _ _ _ _ _ _ EFFLUENT YEAR DAY NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard From I os I -- I - . I 31 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. I! FREQUENCY! SAMPLE EX OF TYPE ANALYSIS MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE ?DAY CHR ******** ******** ******** 23 CERIODAPHNIA 1 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my Timothy P. Cleary direction or supervision 1n accordance with a system designed to assure that qualified personnel properly gather and evaluate the information subrmtted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the UAd TELEPHONE DATE Principal Environmental Engineer informat1on, the Information subm1tted is, to the best of my knowledge and bel1ef, true, 423 843-6700 08 09 11 Site Vice President accurate, and complete. I am aware that there are Significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE mformatJon, including the possibility of fine and Imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXP~NATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUO~~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L .£. ~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _

---~TEROFFICESB-2A) _ _ _ _ _ _ _ _ _ _                                                                        TN0026450                                 116 G         F- FINAL

_ _ _ 20DDY- DAISY _ _]N 3738i_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER BACKWASH Facility_ .JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l._ .J:jAMIL TOJi..COUNTY __________ _ MONITORING PERIOD EFFLUENT ATTN: Stephanie A. Howard From I 08 YEAR DAY 31 NO DISCHARGE CJ *** NOTE Read instructions before completinq this form. PARAMETER NO. IFREQUENCYI SAMPLE EX OF TYPE ANALYSIS IUI:Ct(.J;:), FLOATING (SEVERITY) 0 1 I 31 VISUAL 1 0 0 UENT GROSS VALUE 0 0 ENT GROSS VALUE

                                                                                                                                                  ~\CUU-L(}-~ )Cu.c NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my                                                      I      TELEPHONE                    DATE direct1on or supervision in accordance w1th a system designed to assure that qualified personnel                                                                    ,

Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inqwy of the person or persons who manage the system, or those persons directly responsible for gathering the Princ1pal Environmental Engineer Site Vice President information, the Information submitted 1s , to the best of my knowledge and belief, true, 423 843-6700 08 09 111 accurate, and complete. I am aware that there are sigmficant penalt1es for subm1tting false I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE l NUMBER YEAR MO I DAY j *-- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres.§_ _E.~BOX_1900 ___________ _ TN0026450 117 G F- FINAL ---~TEROFFICESB-~---------- - _ _ _§.ODD.::L- DAISY_ _Iti]7381_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER BACKWASH Facility_ JYA- SEOUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!}_ .J::!AMILTOl::LCOUNTY._ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT ATTN: Stephanie A. Howard From I 08 YEAR DAY 31 NO DISCHARGE D *** NOTE: Read instructions before completinQ this form. PARAMETER NO. I FREQUENCY EX OF ANALYSIS 0 I 1 I 31 IL AND GREASE VISUAL 0 0

                                                                                                                                                       ~~1~ ll~ev-c NAME/TITLE PRINCIPAL EXECUTIVE OFFICER                I Certify under penally of law lhat this document and all attachments were prepared under my                                                    TELEPHONE                     DATE direction or supervis1on m accordance with a system des1gned to assure that qualified personnel Timothy P. Cleary I'~

properly gather and evaluate the information submitted Based on my tnquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer 1nformat1on, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 08 09 11 Site Vice President accurate, and complete I am aware that there are significant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE I I TYPED OR PRINTED I information, including the possJbtl1ty of fine and 1mpnsonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER .YEAR! MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDE$) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres,L __E.~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 118 G F- FINAL ---~TEROFFICESB-~---------- - _ _ _§_ODD..Y_- DAISY_ _IN 37381_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBERl WASTEWATER & STORM WATER Facility _ _s:.JA- SEOUOYAJ:!.NUCLEAR PLANT _ _ _ _ _ _ Locatioll_ .J::!AMIL TOJ:':!_COUNTf_ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT YEAR DAY From NO DISCHARGE I XX I *** ATIN: Stephanie A. Howard 08 To 31 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. /FREQUENCY/ SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS

              , DISSOLVED        (DO)                                     ********                    ********                                                        ********           ********            19 0    0 IDS, TOTAL SUSPENDED 0    0
          , IN CONDUIT OR THRU ITCI:I\TUENT PLANT 0    0 EFFLUENT GROSS VALUE NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary
                                                  ""' ""w direction      '~""

or supervision",_ in accordance -"~"

                                                                                '"" '"" w1t~ a system ' " "' ~oc"m'"" -* ""oo """" m' ,~

desighed to assure t~at qualifted personnel properly gather and evaluate the 1nformat1on submitted Based on my 1nqU1ry of the person or

l.,...LQ_0.4.u,

                                                                                                                                                                                     .      (j1A._.d f----

TELEPHONE DATE personswho manage the system, or those persons directly respons1ble for gathenng the Principal Environmental Engineer Site Vice President mformat1on, the informat1on subm1tted is , to the best of my knowledge and behef, true, accurate. and complete. I am aware that there are signif;cant penalt;es for submitting false 423 I 843-6700 08 09 11 SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED Information, including the possibility of fine and imprisonment for knowing violations

                                                                            ------             ----          --             ----                      I OFFICER OR AUTHORIZED AGENT              AREA CODE l    NUMBER        YEAR      MO     DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

S58 081014 800- NPDES CORRESPONDENCE October 14, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR SEPTEMBER 2008 Enclosed is the September 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN J.D. Smith, OPS 4A-SQN T. P. Cleary, OPS 4A-SQN G. R. Signer, WT 6A-K D. E. Pittman, BR 4T-C EDMS, WT CA-K (Enclosure) DMR0809.doc

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 October 14, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Sect1on 1 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR SEPTEMBER 2008 Enclosed is the September 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Su1te 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR! OMB No. 2040-0004 (SUBR 01) Addres.§__f~BO~OO - - - - - - - - - - - - TN0026450 101 G F- FINAL ---~TEROFFICESB-~---------- ---~DDY-DAIS~~~738L _ _ _ _ _ _ _ PERMIT NUMBER DIFFUSER DISCHARGE Facilitv_ _EA-gQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio..!}_ _I:!AMILTOli_COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard YEAR From1 08 I -- I~ NO DISCHARGE D *** NOTE: Read instructions before completin~ this form. PARAMETER QUANTITY OR LOADING NO. IFREQ EX I OF ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE FF. BETWEEN SAMP. & DEG.C s 0 EFFLUENT GROSS VALUE PH 00400 0 0 EFFLUENT GROSS VALUE TOTAL SUSPENDED

         , IN CONDUIT OR THRU NT PLANT 50050         1      0   0 EFFLUENT GROSS VALUE II Cert1fy under penally of law that th1s document and all attachments were prepared under my
                                                                                                                                                       ~Q_JJR/Wcud I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER                                                                                                                                                                 TELEPHONE                    DATE I

I Timothy P. Cleary direction or supervision m accordance with a system des1gned to assure that qual1f1ed personnel properly gather and evaluate the 1nformat1on subm*tted. Based on my 1nqwy of the person or i i I I . . . persons who manage the system, or those persons d~rectly responsible for gathering the information, the information submttted is , to the best of my knowledge and belief, true, Principal Environmental Engineer 423 843-6700 i 08 10 I 14 Site Vice PreSident accurate, and complele. 1 am aware that there are sigmficant penaltiesfor subm1tting false SIGNATURE OF PRINCIPAL EXECUTIVE I tnformation, tncluding the posstbility of ftne and tmpnsonment for knowtng vtolat1ons. OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY I TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2

DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Dateffime Collected Hydrocarbons Analysis Dateffime Analyst Method 09/10/2008@ 1105 0.28 mg/1 09/12/2008 @ 1552 KLM TN EPA 8015 CCWCHANNEL Extractable Petroleum Dateffime Collected Hydrocarbons Analysis Dateffime Analyst Method 09/10/2008 @ 11 00 <0.10 mg/1 09/12/2008 @ 1604 KLM TN EPA 8015

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Add res.§_ .J: ~BOX_1i)QQ_ _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A) _ _ _ _ _ _ _ _ _ _ TN0026450 101 G F- FINAL _ _ _ _§_ODDY- DAISY_ ___IN 3738£._ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facilitv_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ LocatiO.IL ..J::!AMILTOli_COUNTY __________ _ MONITORING PERIOO EFFLUENT YEAR DAY From I 08 To 30

                                                                                                                                                                           *** NO DISCHARGE           [J ***

ATTN: Stephanie A. Howard NOTE: Read instructions before completinq this form. PARAMETER NO. IFREQUENCYI SAMPLE EX OF TYPE ANALYSIS LORINE, TOTAL RESIDUAL 0 0 RE- C, RATE OF 0 0 _,J~(l.~,o_.(-d NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direct1on or superviSIOn in accordance with a system des1gned to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the Information submitted. Based on my 1nquiry of the person or "E persons who manage the system, or those persons d1rectly responsible for gathering the Principal Environmental Engineer Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, 423 I 843-6700 08

                                                ~~ccurat~, and com~lete. I am a_ware that there a:e si~niftcant penalties_ for submitting false                                                                         i TYPED OR PRINTED (formation. includmg the poss1b1l1ty of fine and 1mpnsonment for know1ng v1olations SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT             AREA CODE l    NUMBER       JYEAR      MO   I DAY_

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured: 1. Biodetergent 73551 (max. calc. cone. was 0.018mg/L--Iimit 2.0mg/L) 2. MSW 101 (max. calc. cone. was 0.059mg/L--Iimit 0.2mg/L) 3. H-150M (max. calc. cone. was 0.038mg/L--Iimit 0.050mg/L) 4. H-150M (low detection level analytical method was <0.020mg/L--Iimit 0.050mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres_L _f..Q;_BOX~OO _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 Q F- FINAL ---~TEROFFICESB-~---------- ---~ODDY-DAIS~~N373BL _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facilitv_ _JYA-_.§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!]_ .J::!AMIL TOli_COUNTX_ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT YEAR DAY NO DISCHARGE [ ] *** ATTN: Stephanie A. Howard From 08 To 30 NOTE Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY oR coNCENTRATION I NO. 1FREauENCYTI'-s-A--=M_P_L_E_ EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS BORON, TOTAL ******** ******** <0.20 19 o I 3192 I 01022 1 0 0 EFFLUENT GROSS VALUE

                                            **. '"fi;f?E"~Mi'l;;  >

Z;;gEQt;llREMENl'<

                                             !&§~

N~o.-~cud NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE i direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information subm1tted. Based on my inquiry of the person or persons who manage the system, or those persons d1rectly responsible for gathering the information. the Information submitted is , to the best of my knowledge and behef, true, Principal Environmental Engineer 423 843-6700 08 10 14 Site Vice President accurate, and complete. I am aware that there are sigmficant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Boron was sampled on 07/02/2008@ 1122, on 07/16/2008@ 1215, and on 07/16/2008@ 1216. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facilitv Name/Locat1on if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres,L _E.~BOX.1.900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 101 T F-FINAL ---~DDY-DAISY_~N3738L _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBERj BIOMONITORING FOR OUTFALL 101 Facility_ .I:JA -_g:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.JL _HAMIL TO_!i_COUNTY__________ _ MON ITOFillillYERlQ_D EFFLUENT From YEAR 08 To DAY 30 NO DISCHARGE D ... ATTN: Stephanie A. Howard NOTE Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. SAMPLE EX TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE ?DAY CHR ******** ******** ******** ******** 23 CERIODAPHNIA 0 0

               ?DAY CHR 0    0
                                                                                                                                                     ~O.~w.cl NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER           I Certify under penalty of law that this document and all attachments were prepared under my                                                     TELEPHONE                    DATE direction or supervision in accordance w1th a system designed to assure that qualified personnel Timothy P. Cleary                   properly gather and evaluate the information submitted. Based on my 1nquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the Information submitted is , to the best of my knowledge and belief, true,                                                    423       843-6700        08        10     14 Site Vice President                  accurate, and complete. I am aware that there are s1gnif1cant penalties for submitting false       SIGNATURE OF PRINCIPAL EXECUTIVE                I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA CODE I

                                                                                                                                                                                                     !   NUMBER        YEAR      MO      DAY 1 I

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in September 2008. EPA Form 3320-1 (REV 3/99) Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Add res§_ ..f..Q_BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 F- FINAL ---~TEROFFICESB-~---------- - _ _ _§_ODDY- DAISY_ _]~738'!_ _ _ _ _ _ _ _ LOW VOL. WASTE TREATMENT POND Facility_ ....JYA -_g':QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!:!_ _!::!AMIL TO.!:!_COUNTY __________ _ EFFLUENT NO DISCHARGE [ ] *** ATTN: Stephanie A. Howard NOTE Read instructions before completinq this form. PARAMETER 0 0 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 00556 1 0 0 EFFLUENT GROSS VALUE ORTHRU PLANT 0 0

                                                                                                                                                      ~(}.~v,J NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER             I Certify under penalty of law that this document and all attachments were prepared under my                                                   TELEPHONE                    DATE direction or superv1sion in accordance with a system designed to assure that qualified personnel Timothy P. Cleary                properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer                                               14 information, the information submitted is , to the best of my knowledge and belief, true,                                                  423       843-6700        08       10 Site Vice President               accurate, and complete I am aware that there are significant penalties for submitttng false      SIGNATURE OF PRINCIPAL EXECUTIVE                I TYPED OR PRINTED information, including the possibility of fine and tmprisonment for knowing violations             OFFICER OR AUTHORIZED AGENT            AREA   I   NUMBER        YEAR      MO  DAY I                                             _:...._____ _________                                                   -

CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) MAJOR Name_~A-SEQUOYA~UCLEA~LANT ___ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _E ~BOX.AJQQ_ ___________ _ ---~TEROFFICESB-~---------- TN0026450 107 G F-FINAL - _ _ _§.ODDY- DAISY_ _IN 3738!_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER I METAL CLEANING WASTE POND Facility_ .JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!!_ _!:!AMIL TOli_COUNTY__________ _ MONITORING PERIOD EFFLUENT YEAR DAY NO DISCHARGE @] ... A TIN: Stephanie A. Howard From 08 To 30 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE 0 0 1 0 0 ENT GROSS VALUE 1 0 0 EFFLUENT GROSS VALUE PHOSPHORUS, TOTAL (AS P) 00665 1 0 0 EFFLUENT GROSS VALUE 0 0 EFFLUENT GROSS VALUE IRON, TOTAL (AS FE)

         , IN CONDUIT OR THRU NT PLANT 0      0
                                                                                                                                                      ~~(l~y,~

NAMErriTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the informat1on submitted is , to the best of my knowledge and belief, true, Principal Environmental Engineer 423 843-6700 08 10 14 Site Vice President accurate, and complete. I am aware that there are sigmf1cant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE I I Information, including the possibility of fine and imprisonment for knowing violations. DAY-j TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO I CODE I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Add res.§_ _E.~BOJ<.1900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB*~---------- TN0026450 110 G F- FINAL - _ _ _§_ODD..X.: DAISY_ _IN 37381.._ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facilitv_...I:JA-..§l':QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!]_ JjAMIL TOJ:::!..COUNTY._ _ _ _ _ _ _ _ _ _ _ EFFLUENT YEAR DAY NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard From 08 30 NOTE: Read instructions before completinq this form. PARAMETER QUALITY OR CONCENTRATION I NO. IFREQUE SAMPLE EX OF TYPE ANALYSIS MAXIMUM UNITS RE, WATER DEG. if't:II.ITI~RADE 04 z 0 0 0 0 DUITORTHRU PLANT 0 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL 50060 0 0 FFLUENT GROSS VALUE

                                                                                                                                                     ~0~C~1d.

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel J Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly respons1ble for gathering the Site Vice President informat1on, the informatmn submitted is , to the best of my knowledge and belief, true, Principal Environmental Engineer 423 I 843-6700 08 10 I 14 accurate, and complete. I am aware that there are significant penalties for submitting false I SIGNATURE OF PRINCIPAL EXECUTIVE i 1nformat1on, Including the possibility of ftne and imprisonment for know1ng vtolations OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO I DAY_ TYPED OR PRINTED I CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used

PERMITTEE NAME/ADDRESS (Include Facifitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR! OMB No. 2040-0004 (SUBR 01) Addres_L _E.~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A) _ _ _ _ _ _ _ _ _ _ TN0026450 110 T F- FINAL _ _ _ _§ODDY- DAISY_ _IN 373BL _ _ _ _ _ _ _ PERMIT NUMBER I I DISCHARGE NUMBER RECYCLED COOLING WATER Facility_ JYA -_§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio_Q_ .J:!AMIL TOJi.COUNTY__________ _ EFFLUENT DAY NO DISCHARGE I XX I *** ATTN: Stephanie A Howard 30 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION / NO. I FREQUENCY! SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR ******** ******** *****"If** ******** 23 CERIODAPHNIA TRP3B 0 0 ~~~~~~f 45.2 i **... ., SEMI I.'C:PIIAPOS !EFFLUENT GROSS VALUE ~;~-MINiMUM fie25 STATRE 7DAY CHR lPIMEPHALES ITRP6C 1 0 0 -CGMG!.c>S,

                                                                                                                                                                                                                                                  - '~

EFFLUENT GROSS VALUE 1 I NAM~ITIT_~ PRIN~If:l~L EXE_CU:TW~-~~~-~!'_j~~=~',~nu~rd:~:.e~~~:;no:~aa:~~~~~~~ed~~~:es~s~~~ ~~:~~~:~~~t=s~~:~:;te~~~~~eu;~:~s~~nel I IJ-. ~ L .. : Ttmothy P Cleary !properly gather and evaluate the 1nformat1on submitted Based on my 1nqwy of the person or ~~~{.A~ A /) j / ___ w.d ~- __ _2'_~~EP~O__"J_~-------1----~~~~----- 1 -~

                                                     .persons who manage the system, or those persons d1rectly responsible for gathenng the                   Prtnctpal Envtronmental Engineer         1
                  .                                  l1nformat1on. the 1nforma\lon submitted 1s to the best of my knowledge and belief. I rue,                                                      --i 423         843-6700        08    :    10 Stte Vtce President        =-=['accurate. and complete I am aware that there are s'gn1f1cant penalt1es for subm1tt1ng false            I--SIGNATURE OF PRINCIPAL EXE.CUTNE 1------===----c:-=--=:-c-==------               -----l:nformat1on, :nclud1ng the poSSibility of f1ne and 1mpnsonment for know1ng violat1ons            I       OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED         ,                                                                                                             _                                                 ~~~-

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Faci/itv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Na~-~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres_L _f.~BOX_19QQ_ _ _ _ _ _ _ _ _ _ _ _ _ -~-~TEROFFICESB-~---------- TN0626450~ 116 G F- FINAL - _ _ ....§.ODD..Y_- DAISY_ _]1::!.]7381._ _ _ _ _ _ _ _ PERMIT NUMBER= I DISCHARGE NUMBER BACKWASH Facility_ .JYA -_§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l._ _!:!AMIL TOJ::!...COUNTY __________ _ MONITORlNG PERIOD EFFLUENT To 08 r YEAR MO 09 DAY 30 NO DISCHARGE D ... ATTN: Stephanie A. Howard 1 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUE SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS

                                                                                          ********                   ********                                ********            ********             0                9A         0 0       0 FFLUENT GROSS VALUE IL AND GREASE VISUAL 0       0 ENT GROSS VALUE l_!iAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision 1n accordance with a system des1gned to assure that qual1fied personnel I                     Timothy P. Cleary                            properly gather and evaluate the 1nformatton submitted Based on my inqUiry of the person or persons who manage the system, or those persons d1rectly responsible for gathering the Principal Environmental Engineer 423 I                    Site Vice President 1nformat1on, the informatiOn submitted IS , to the best of my knowledge and bel1ef, true, accurate, and complete. I am aware that there are Significant penalties for subm1tt1ng false         SIGNATURE OF PRINCIPAL EXECUTIVE r - - - - - - - - - - - - - - - - - - - - - - - - - 1 i n f o r m a t 1 o n , Including the possibility of fine and imprisonment for knowing VIOlations                   OFFICER OR AUTHORIZED AGENT             AREA TYPED OR PRINTED                                                                                                                                                                             CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS                                  (Reference all attachments here)

Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different! NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) AddresL _E.~BOX..lQOO _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 117 G F- FINAL ---~TEROFFICESB-~---------- - _ _ _§ODD_i_- DAISY_ _IN 37384_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER BACKWASH Facility_ JYA -_§!:.QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!!_ _I:!AMIL TOl!.__COUNTY __________ _ MONITORING PERIOD EFFLUENT DAY NO DISCHARGE [ ] *** ATIN: Stephanie A Howard To 30 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION \ NO. IFREQUENCY SAMPLE I EX OF ANALYSIS TYPE I AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS

                                                                                                                                                                                                                  +-------+-----t-----,----1 i

loesRIS, FLOATING (SEVERITY) ******** ******** ******** ******** 0 0 1/30 VISUAL 9A 01345 0 0 R.ERORl :"1 },;:9J:t~* ';J,W$UA4. EFFLUENT GROSS VALUE ,l~ik~M-o.JeiJ\L::* OIL AND GREASE VISUAL ******** VISUAL 84066 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my L¥U.UVIcuuia.~c:cr ~--

                                                                                                                                                                                                                             ~-----DATE          I
                                                                                                                                                                                                                             ~.r=:-r:

d1rection or supervision 1n accordance w1th a system des1gned to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the InformatiOn submitted Based on my inquiry of the person or persons who manage the system, or those persons d1rectly responsible for gathering the Principal Environmental Engineer 423 843-6700 information. the information submitted is . to the best of my knowledge and belief, true, r-1 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE informat1on, including the possibility of fine and 1mpnsonment for know1ng v1olat10ns. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ MAJOR Form Approved Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT !DMR) OMB No. 2040-0004 (SUBR 01) .fu!dres.§_ _f.~BOX.lQQQ_ _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 118 G F- FINAL - _ _ _§_ODD_1_- DAISY_ ____Tt:!]738i._ _ _ _ _ _ _ _ WASTEWATER & STORM WATER Facility_ JYA -_§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio!]_ _ljAMIL TO_!::!_COUNTY __________ _ EFFLUENT NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard. From NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. EX IFREQUENCY OF I SAMPLE TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO) ******** ******** ******** ******** 19 00300 1 0 0 1EFFLUENT GROSS VALUE FIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS VALUE SOLIDS, SETTLEABLE i 00545 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 0 0 .:REPOFir**-,1 MGD EFFLUENT GROSS VALUE ;;~Jl;"i~;~All£Yc,M~~~~:

                                                                                                                                                  ~Ot~)N_u.f NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this document and all attachments were prepared under my                                                            TELEPHONE                    DATE
                                                ~~irection or superv1sion 1n accordance with a system designed to assure that quallf1ed personnel                                                                     -

Timothy P. Cleary properly gather and evaluate the 1nformat1on submitted. Based on my inqUiry of the person or Ipersons who manage the system, or those persons d1rectty responsible for gathenng the Principal Environmental Engineer

                  .    .        .                tnformation, the Information submitted is , to the best of my knowledge and belief, true,                                                    423       843-6700         08      10     14 S1te V1ce PreSident               accurate, and complete 1am aware that there are significant penalt1es for submitting false        SIGNATURE OF PRINCIPAL EXECUTIVE                  I I

TYPED OR PRINTED information, mcludmg the possibility of fine and 1mprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA CODE l NUMBER IYEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous may be used Page 1 of 1

S58 081113 800 - NPDES CORRESPONDENCE November 13, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR OCTOBER 2008 Enclosed is the October 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 28-SQN J.D. Smith, OPS 4A-SQN T. P. Cleary, OPS 4A-SQN G. R. Signer, WT 6A-K D. E. Pittman, BR 4T-C EDMS, WT CA-K (Enclosure) DMR0810.doc

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 November 13, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR OCTOBER 2008 Enclosed is the October 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington. D.C. 20555

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres.§__E~BOX_2900 - - - - - - - - - - - - ---~TEROFFICESB-~---------- TN0026450 101 G F- FINAL _ _ _ _.§.ODDY- DAISY_ ____IN 37381._ _ _ _ _ _ _ _ DIFFUSER DISCHARGE Facility_ ~A -..§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!}_ .J::!AMILTO..!::!_COUNTY __________ _ EFFLUENT YEAR DAY NO DISCHARGE [ ] *** ATTN: Stephanie A. Howard From I 08 I -- I -

I 31 NOTE: Read instructions before completinq this form.

PARAMETER QUALITY OR CONCENTRATION NO. EX UNITS MINIMUM AVERAGE MAXIMUM UNITS PERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** MEASUREMENT 26.4 04 0 NTIGRADE 00010 z 0 0 INSTREAM MONITORING TEMPERATURE, WATER DEG. ******** ******** CENTIGRADE 00010 0 0 EFFLUENT GROSS VALUE BETWEEN SAMP. & DEG.C s 0 0 0 0 0 ENT GROSS VALUE 0 0 0 0 EFFLUENT GROSS VALUE

                                                                                                                                                          ~O~cuc NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my                                                                       TELEPHONE                    DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary                     properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true,                                                      423      843-6700         08       11  13 Site Vice President                    accurate, and complete. I am aware that there are significant penalties for submitting false        SIGNATURE OF PRINCIPAL EXECUTIVE                 I TYPED OR PRINTED Information, including the possibifity of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2

DMR Attachment CCW Data CCWTRENCH Extractable Petroleum DaterTime Collected Hydrocarbons Analysis DaterTime Analyst Method 10/08/2008 (52 1154 0.36 mg/1 10/13/2008@ 1636 KLM TN EPA 8015 CCWCHANNEL Extractable Petroleum DaterTime Collected Hydrocarbons Analysis DaterTime Analyst Method 10/08/2008 @ 1151 0.10 mg/1 10/13/2008@ 1646 KLM TN EPA 8015

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ MAJOR Form Approved Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRI OMB No. 2040-0004 (SUBR 01) Addres_L _E Q,_BOX.1.900 _ _ _ _ _ _ -'- _ _ _ _ _ TN0026450 101 G F *FINAL ---~TEROFFICESB-~---------- ---~ODDY-DAISY_~N3738L _ _ _ _ _ _ _ DIFFUSER DISCHARGE Facilitv_ _IYA-~QUOYAH NUCLEAR PLANT_ _ _ _ _ _ LocatiOJL __!:!AMIL TOJ:i.COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard From I YEAR os I . - I - - I DAY 31 NO DISCHARGE CJ *** NOTE: Read instructions before completinq this form. PARAMETER NO. IFREQUE EX OF ANALYSIS UNITS RINE, TOTAL RESIDUAL 0 19 0 0 RE

C, RATE OF 0

_____________ . ___________ ------ _______ -----

penaltyoflawthatthisdocumentandallattachmentswerepreparedundermy f--------------'--'----'---".c:__----11direction or super;1sion in accordance with a system designed to assure that qualified personnel gather and evaluate the informatiOn submitted. Based on my 1nquiry of the person or' L/.1~

who manage the system, or those persons directly responsible for gathering the

                                                   \information, the information submitted is, to the best of my knowledge and belief, true, cipal Environmental Engineer                  843-6700      I 08 I      11 I 13 Site Vice President                  accurate, and complete. I am aware that there are significant penalties for submitting false    SIGNATURE OF PRINCIPAL EXECUTIVE

! TVot:n f"'\o ~ Tcn 001 1 Iinformation, including the possibility of fine and impnsonment for knowing violat1ons. OFFICER OR AUTHORIZED AGENT NUMBER IYEARI MO I DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured: 1. Biodetergent 73551 (max. calc. cone. was 0.021 mg/L--Iimit 2.0mg/L) 2. MSW 101 (max. calc. cone. was 0.061 mg/L--Iimit 0.2mg/L) 3. H-150M (max. calc. cone. was 0.041 mg/L--Iimit 0.050mg/L) 4. H-150M (low detection level analytical method was <0.020mg/L--Iimit 0.050mg/L) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L ...£. Q.,_BOX 20QQ_ _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 101 T F-FINAL F=-~-~,;,;;,.;,.,_,;,..,.,.~ - _ _ _§_ODDY- DAISY_ _IN 37381._ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facility_ .JYA -_g:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ locatio.n_ ....!:!AMILTO.!i_COUNTY__________ _ MQNIIQB.J..f'.I.G_E'E RIOD EFFLUENT From YEAR 08 To I YEAR I 08 MO 10 I DAY 31 NO DISCHARGE D ... ATIN: Stephanie A. Howard NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION \ NO. \FREQUENCY\ SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS

                                                                          ********                   ********                                >100.0              ********          ********            23 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my Timothy P. Cleary Site Vice President direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, o.

Principal Environmental Engineer 423 TELEPHONE 843-6700 08 DATE 11 13 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED information, includmg the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE NUMBER YEAR MO I DAY

                                                                                                                    ~~----

COMMENTS AND EXPLANATION OF AtiiY VIOLATIONS (Reference all attachments here) Toxicity was sampled October 26-31, 2008. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

November 13, 2008 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450,, COMPLIANCE TOXICITY TESTS, OCTOBER, 2008 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101, samples collected October 26-31, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated OutfalllOl and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment): Sherrard, R. M., PSC IX-C Files, ER&TA, CEB IB-M SQN October 2008m

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: November I3, 2008 I. Facility I Discharger: Seguoyah Nuclear Plant I TVA

2. County I State: Hamilton I Tennessee

3. NPDES Permit#: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1,579

6. Receiving Stream: Tennessee River (TRM 483.6)

7. IQIO: 3,49I

8. Outfall Tested: I 0 I

9. Dates Sampled: October 26-31, 2008

10. Average Flow on Days Sampled (MGD): I575, I555, 1554 1I. Pertinent Site Conditions: H-I50M was injected into the Raw Cooling Water (RCW) System starting on October 28, 2008 at 1120 and ended on October 31, 2008 at I255.

12. Test Dates: October 28-November 4, 2008

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia) I5. Concentrations Tested(%): Outfall I 01: Il.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephales promelas: UV treated Outfall I OI: I1.3, 22.6, 45.2, 72.6, 100 UV treated Intake: I 00.0

16. Permit Limit Endpoint(%): Outfall I 01: IC 25 = 45.2%

I7. Test Results: Outfall I 01: Pimephales promelas: IC 25 > I 00% Ceriodaphnia dubia: ~5 > I 00% UV treated Outfall I 0 I: Pimephales pro me/as: ~5 > 100% Page 1 of96

18. Facility

Contact:

Stephanie Howard Phone#: (423) 843-6713

19. Consulting I Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Cynthia L. Russell *Phone#: (256) 386-2755

22. Notes: Outfall 101 samples collected Octqber 26-31, 2008, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were

               > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Fathead minnows were also exposed to tJv treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

Page 2 of96

                                               ~-------------

METHODS

SUMMARY

Samples:

1. Samphng Point: Outfall 101, Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MMIDD/YY)/ (MM/DD/YY)/ Arrival Initial (MM/DD/YY)/ Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (OC) (mg/L) First Used By I 0/26/08 0742 to I 0/28/08 1647 101 I0/27/08 I456 1.3/2.1 t <0.10 10/27/08 0642 I 0/29/08 1549 I 0/26/08 0816 to I 0/28/08 1647 Intake 10/27/08 1456 1.7 <0.10 10/27/08 0716 10/29/08 1549 10/28/08 0809 to 10/30/08 1630 10I 10/29/08 1423 0.8/1.5t <0.10 10/29/08 0709 10/31/08 I550 I 0/28/08 0826 to 10/30/08 1630 Intake 10/29/08 1423 0.5 <0.10 10/29/08 0726 10/31/08 1550 I1/01/08 1548 10/30/08 0802 to 101 10/31/08 1352 1.8/2.8t <0.10 11102/08 1605 10/31/08 0702 11/03/08 I553 Il/0 1/08 1548 10/30/08 0834 to Intake 10/31/08 1352 1.6 <0.10 Il/02/08 1605 10/31/08 0734 11/03/08 1553

            *TRC =Total Residual Chlorme tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall! 01 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath.

Aliguots of Outfall 101 and Intake samples were UV ~treated through a 40~watt SmartUV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Page 3 of96

Pimephales promelas Ceriodaphnia dubia Test Organisms:

1. Source: Aguatox, Inc. In-house Cultures

2. Age: 24.55-24.78 hours old <24-hours old Test Method Summary:

1. Test Conditions: Static, Renewal Static, Renewal

2. Test Duration: Until at least 60% of control females have 3 broods

3. Control I Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic

4. Number of Replicates: 4

5. Organisms per Replicate: lQ 1

6. Test Initiation: (Date/Time)

Outfall101 10/28/08 1647 ET 10/28/08 0800 ET UV Treated Outfall 101 10/28/08 1633 ET

7. Test Termination: (Date/Time)

Outfall 101 11/04/08 1556 ET 11/04/08 0705 ET UV Treated Outfall 101 11/04/08 1540 ET

8. Test Temperature: Outfall I 01: Mean= 24.8°C Mean= 24.8°C (24.5 - 25.1 °C} (24.6 - 25.1 °C}

Test Temperature: UV-Treated OutfalllOl: Mean = 24.8°C (24.6- 25.1 °C}

9. Physical I Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).

Page 4 of96

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted October 28- November 04,2008 using effluent from Outfall101. Test Percent Surviving Solutions (time interval used- d~ (%Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 98 98 Intake 100 100 100 100 98 95 93 Mean Dry Weight (mg) Test Solutions (replicate numbei} (% Effluent) 1 2 3 4 Mean Control 0.707 0.655 0.717 0.672 0.688 11.3% 0.591 0.692 0.691 0.693 0.667 22.6% 0.664 0.709 0.613 0.650 0.659 45.2% 0.586 0.630 0.760 0.688 0.666 72.6% 0.657 0.617 0.577 0.724 0.644 100.0% 0.635 0.565 0.645 0.593 0.610 Intake 0.679 0.758 0.569 0.622 0.657 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

              *TUa = 100/LCso: TUc = 100/ IC2s Page 5 of96

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted October 28- November 04,2008 using effluent from Outfall101. Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (%Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 35 36 34 34 31 33 35 33 33 33.3 11.3% 34 35 34 38 35 31 37 34 37 31 34.6 22.6% 37 38 34 36 36 36 37 35 37 31 35.7 45.2% 32 38 37 39 34 36 35 39 37 36 36.3 72.6% 38 40 39 37 34 35 39 36 38 38 37.4 100.0% 43 37 39 37 38 38 42 39 37 42 39.2 ICzs Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

              *TUa = 100/LC 50 : TUc = 100/ IC 25 Page 6 of96

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted October 28- November 04, 2008 using water from Intake Percent Surviving Test (time interval used- days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 36 30 34 32 33 31 32 34 31 32.3 Intake 35 31 34 36 33 32 34 29 35 31 33.0 lCzs Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

              *TU a = 100/LCso: TU c = 100/ ICzs Page 7 of96

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted October 28-November 04, 2008 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used- days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg) Test Solutions replicate number) (% Effluent) 1 2 3 4 Mean Control 0.489 0.416 0.440 0.502 0.462 11.3% 0.578 0.614 0.632 0.622 0.612 22.6% 0.503 0.489 0.604 0.606 0.551 45.2% 0.575 0.540 0.547 0.639 0.575 72.6% 0.558 0.579 0.663 0.688 0.622 100.0% 0.556 0.557 0.541 0.603 0.564 Intake 0.581 0.660 0.592 0.673 0.627 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

                *TUa = 100/LCso: TUc = 100/ ICzs REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC2s) P. promelas October 28- November 04, 2008 1700 7-days KCl 0.79 giL C. dubia October 07 - 14, 2008 0815 7-days NaCl 1.09 g/L Page 8 of96

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pimephalespromelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant Effluent (SQN) October 28- November 4, 2008 Test Sample ID Temperature ("C) Dissolved Oxygen (mg!L) pH (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (f.lmhos/cm) (mg/L CaCO~ (mg/L CaC0 3) Chlorine (mg/L) Control 24.8 24.7 7.9 7.5 7.64 7.37 306 60 92 - 24.7 - 24.8 24.6 - 24.8 7.7 - 8.2 6.3 - 8.0 7.41 - 7.90 7.19 - 7.51 296 - 316 60 61 85 - 96 11.3% 24.8 24.8 8.0 7.5 7.63 7.35 289 - - -

                  ..,                        24.7 - 25.0  24.6 - 25.0   7.8    - 8.2    6.1       8.0 7.50 - 7.90   710 - 7.49    277 - 302      -        -    -         -      -         -
                ..:!                             24.9         24.6            8.0            7.5          7.63           7.34         275           -               -                 -

22.6% 24.7 - 25.0 24.5 - 24.8 7.8 - 82 6.2 - 8.0 7.48 - 7.91 709 - 7.49 260 - 284 - - 24.9 24.7 8.0 7.5 7.60 7.35 252 - - -

                ..::""'            45.2%

24.7 - 25.0 24.5 - 24.9 7.7 - 8.2 6.3 - 8.0 7.45 - 7.88 7.15 - 7.48 240 - 263 - - - -

                ~ "'                             24.9         24.8            8.0            7.5          7.60           7.35         224           -               -                 -
                .§ e-               72.6%

24.8 - 25.0 24.7 - 24.9 7.8 - 8.3 6.4 8.0 7.45 - 7.86 7.10 - 7.50 207 - 242 - - -

                >l...                            24.9         24.8            8.1            7.5          7.59           7.35         197          67              83              < 0.10 100.0%

24.8 - 25.1 24.6 - 24.9 7.9 - 8.3 6.3 - 8.0 7.45 - 7.85 7.08 - 7.52 182 - 215 66 - 68 75 - 92 < 0.10 - <0.10 24.9 24.8 8.1 7.6 7.58 7.35 204 68 81 < O.ID Intake 24.8 - 25.0 24.6 - 24.9 7.8 - 8.4 6.4 - 8.0 7.42 - 7.83 7.08 - 7.53 177 - 283 64 - 74 79 - 83 < 0.10 - <0.10 Control 24.7 24.8 7.9 7.9 7.64 7.52 306 60 92 - 24.7 - 24.9 24.6 - 25.0 7.7 - 8.2 7.4 - 8.2 7.41 - 7.90 7.40 - 7.66 296 316 60 - 61 85 96 - 11.3% 24.8 24.9 8.0 7.9 7.63 7.54 289 - - - 24.7 - 24.9 24.7 - 25.1 7.8 . 8.2 7.3 - 8.2 7.50 - 7.90 7.41 - 7.66 277 - 302 - . . . .

.~

24.8 24.9 8.0 7.9 7.63 7.53 275 - - -

                  ~                22.6%

24.7 . 25.0 24.7 . 25.1 7.8 8.2 7.3 - 8.2 7.48 . 7.91 7.40 - 7.65 260 . 284 - -

.~

0:: 24.8 24.8 8.0 7.9 7.60 7.53 252 - - -

                ~                  45.2%

24.7 - 25.0 24.6 - 25.0 7.7 8.2 7. 3 8.2 7.45 . 7.88 7.39 . 7.64 240 263 -

                   ~
                 "'l
                 .;:               72.6%

24.8 24.8 8.0 7.9 7.60 7.52 224 - . -

                  ;;;                        24.7 . 25.0  24.6 . 25.0    7.8        8.3 72        8.2 7.45 - 7.86    7.39 - 7 65  207     242                  .         -

(._) 24.9 24.8 8.1 7.9 7.59 7.52 197 67 83 < 0.10 100.0% . . 24.8 . 25.0 24.7 . 25.0 7.9 8.3 7.3 - 8.2 7.45 . 7.85 7.40 - 7.66 182 . 215 66 68 75 92 < 0.10 - < 0.10 24.9 24.8 8.1 7.9 7.58 7.51 204 68 81 < 0.10 Intake . L___ - - - - 24.7 . 25.0 24.7 . 25.1 7.8- - .- -8.4 7.3 ~ 7.42 - 7.83 7.41 . 7.65 177 . 283 64 74 79 - 83 < 0.10 . < 0.10 Overall temperature ('C) Average Minimum Maximum Pimephales promelas 24.8 24.5 25.1 Ceriodaphnia dubia 24.8 24.6 25.1 Page 9 of 96

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pimephales pro me las Tests, UV -treated Sequoyah Nuclear Plant Effluent (SQN October 28- November 4, 2008 Test Sample ID Temperature ("C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Initial Final Initial Final Initial Final (J.lmhos/cm) (mg/L CaCOJ) (mg/L CaC0 3) 24.8 24.7 8.0 7.6 7.61 7.40 294 60 90 Control 24.8 - 24.9 24.6 - 24.8 7.8 - 8.2 6.6 - 8.1 7.43 - 7.88 7.16 - 7.57 280 - 303 59 - 61 85 - 96 11.3% 24.9 24.9 8.1 7.6 7.62 7.37 288 - - 24.8 - 25.0 24.6 - 25.0 7.9 - 8.2 6.6 - 8.0 7.46 - 7.87 7.15 - 7.54 274 - 300 - - - -

               ..;::"'                   24.9          24.8           8.1              76          7.62           7.38          275               -          -
                 !:"'Q      22.6%

24.8 - 25.1 24.6 - 24.9 7.9 - 8.2 6.6 - 8.0 7.46 - 7.86 7.16 - 7.55 266 - 288 - - - -

                 ...C).,

45.2% 25.0 24.7 8.1 7.6 7.61 7.37 253 - -

               ~                     24.9 - 25.1   24.6 - 24.9   8.0   -   8.3   6.7    -  8.0 7.46 - 7.85    7.16 - 7.54   242 - 263        -        -  -       -
               ~
                 &          72.6%

25.0 24.8 8.1 7.6 7.60 7.36 226 - -

               .§                    24.9 - 25.1   24.6 - 24.9   7.9   -   8.3   6.7    -  8.0 7.46 - 7.82    7.13 - 7.55   217 - 235        -        -  -       -
               ~

25.0 24.7 8.1 7.6 7.60 7.36 193 70 83 100.0% 24.9 - 25.1 24.6 - 24.9 8.0 - 8.3 6.6 - 8.1 7.46 - 7.81 7.14 - 7.57 181 - 204 64 - 80 79 - 87 24.9 24.7 8.1 7.6 7.58 7.37 189 71 75 Intake 24.9 - 25.0 24.6 - 24.8 7.8 - 8.3 6.6 - 8.1 7.45 - 7.78 7.14 - 7.57 170 - 202 66 - 80 64 - 81 Overall temperature ('C) Average Minimum Maximum Pimephales promelas 24.8 24.6 25.1 Page 10 of96

SUMMARY

I CONCLUSIONS Outfall 101 samples collected October 26-31, 2008, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were

             > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 11 of 96

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None

2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

2. Ceriodaphnia dubia None Page 12 of96

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B.

4. Dissolved oxygen was measured by SM 4500 0 G.

5. The pH was measured by SM 4500 H+ B.

6. Conductance was measured by SM 251 0 B.

7. Alkalinity was measured by SM 2320 B.

8. Total hardness was measured by SM 2340 C.

9. Total residual chlorine was measured by ORION Electrode Method 97-70.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in giL KCl or NaCl.

2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 13 of96

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity ofEffluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Standard Methods for the Examination ofWater and Wastewater, 20th Edition, 1998.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 14 of 96

Sequoyah Nuclear Plant Biomonitoring October 28 -November 4, 2008 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 96

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall I 01) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Samphng, March 12, 1998 -October 31, 2008 PCL-401 mg/L Copolymer 0.005 0.011 0.021 0.019 0.015 0.015 0.015 0.024 0.024 0.024 0.024 0.024 0.024 0.023 Page 16 of 96

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998-0ctober31,2008 Date PCL-401 mg/L Copolymer 0 1131/2 000 .*. 0.009 02/01/2000 0.028 02/02/2000 0.009 02/03/2000 *.. 0.009 02/04/2000 . 0.009 02/05/2000 0.009 02/06/2000 0.009 07/26/2000 0.019 07/27/2000 0.019 07/28/2000 0.018 07/29/2000 0.019 07/30/2000 0.019 07131!2000 0.019 08/01/2000 0.019 12111/2000 0.020 12112/2000 0.020 12113/2000 0.020 12114/2000 0.020 12115/2000 0.020 12116/2000 0.020 12117/2000 0.020 08/26/2001 0.021 08/27/2001 0.021 08/28/2001 0.021 08/29/2001 0.020 08/30/2001 0.021 08/31/2001 0.020 11/25/2001 11126/2001 0.02 11/27/2001 0.019 11128/2001 . 0.019 11129/2001 0.02 11130/2001 0.02 12/09/2001 .* 12110/2001 *..* 12111/2001 12/12/2001 .* 0.02 12/13/2001 .* 0.02 12114/2001 *..

0.02 Page 17 of96

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -October 31,2008 Date PCL-401 mg/L Copolymer 0.02 0.014 0.014 0.014 0.023 0.023 0.023 0.008 0.008 05/05/2002 05/06/2002 0.02 05/07/2002 0.02 05/08/2002 0.019 05/09/2002 0.02 05110/2002 0.019 08/04/2002 08/05/2002 0.018 08/06/2002 0.018 08/07/2002 0.019 08/08/2002 0.019 08/09/2002 0.018 10/06/2002 10/07/2002 0.018 10/08/2002 0.018 10/09/2002 0.018 10/10/2002 0.018 10111/2002 0.018 01112/2003 01113/2003 0.019 01/14/2003 0.020 01115/2003 0.020 01116/2003 0.020 01117/2003 0.009 04/06/2003 / . .* .. 04/07/2003 0.021 04/08/2003 0.021 04/09/2003 0.021 04/10/2003 0.021 0411112003 0.022 Page 18 of96

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 -October 31, 2008 Date PCL-401 mg/L Copolymer 06/15/2003 06/16/2003 0.020 06117/2003 0.014 06/18/2003 0.014 06/19/2003 .... 0.020 06/20/2003 . 0.020 08/03/2003 08/04/2003 . 0.020 08/05/2003 0.020 08/06/2003 0.020 08/07/2003 0.020 08/08/2003 0.020 10/05/2003 0.020 10/06/2003 0.020 10/07/2003 0.020 10/08/2003 0.020 10/09/2003 0.022 10/10/2003 0.024 0.009 0.009 0.009 0.009 0.009 0.009 0.019 0.014 0.013 0.020 0.021 0.020 0.019 0.020 0.020 0.019 0.019 0.020 Page 19 of96

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall! 01) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -October 31,2008 Date 11/07/2004 11/08/2004 11/09/2004 11/10/2004 11/11/2004 11/12/2004 02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/1112005 06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10/2005 07117/2005 07118/2005 07/19/2005 07/20/2005 07/21/2005 07/22/2005 10/30/2005 10/3112005 11/01/2005 11/02/2005 11/03/2005 11/04/2005 11/14/2005 11/15/2005 11/16/2005 11/17/2005 11/18/2005 11/19/2005 Page 20 of96

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12,1998 -October 31,2008 Date Cuprostat-PF

MSW mg/L 101 Azole 11112/2006 11/13/2006 11114/2006 11115/2006 11116/2006 11/17/2006 11/26/2006 11/27/2006 11/28/2006 11/29/2006 11/30/2006 12/01/2006 05/28/07 0.015 05/29/07 0.015 05/30/07 0.015 05/31/07 O.D15 06/01/07 0.015 06/02/07 O.D15 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/13/08 04/14/08 04115/08 04116/08 04117/08 04/18/08 10/26/08 10/27/08 10/28/08 10/29/08 0.030 10/30/08 0.030 10/31/08 0.030 Page 21 of96

Sequoyah Nuclear Plant Biomonitoring October 28 -November 4, 2008 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 22 of96

"'ti a BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1

 ~
 ~Client: TV A                                                             Environmental Testing Solution, Inc.                                 Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 3 51 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comme~ ~ / J../~d_ /"' Chevy Williams: 'fA AJ tL_ Phone: 828-350-9364 Roy Quinn ~ luA~ NPDES Number: TN0026450 7 Fax: 828-350-9368 Dissolved Metals Collected. Samples remained on ice Collected By: Chevy Williams, Roy Quinn through out sampling and transport to lab. Field Identification I Grab/Comp. Collection Date/Time Container Flow Rain Event? 5058. ptLOj a.J~ Sample Description Number& MGD Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-Inches Number Ten\p . anee E\ (oC) t:\ SQN-101-TOX Comp 10/26/08-10/27/08 0742- 2 (2.5gal) 1573- X 0010)'-:f.Ol 0642 1577 I* 3/-z.\' ~o\ \>\~~ ~ AVG= 1575 SQN-INT-TOX Comp 10/26/08-10/27/08 0816- 1 (2.5 gal) NA X ()6;01(1-0Z. 0716

                                                                                                                                                                              \ .1'(...

a' ,J.SI... ~ Sample Custody Fill In From Top Down -It Wo~~ ~o-J. $ ,Q, A~>" ....d Relinquished By (Signature): Date/Time Received By (Signature): lA.~ Date/Tim()/

                                                                                                                                                                                                                ..... A...Jl Chevy Williams                                                10/27/08                               Sonic Delivery                                                 10/27/08 I o: 3Dc50T
                                )WfA_                                                                       ~!f,.-z.~    ,

10 ~ :;CJ EIJT Sonic Deli~~ ~ ~" £. 10/27/08 10/27/08

                                                                                      ,q 'S\c,. E:.\      ETS *11            Jl                                                              1'-\Sto E \
                    ~TK...           "' ,..,_. -Jl...:>                                                             r~L
                                                                                                                    \..J    I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

'"d ~

 ~                                                                         BIOMONITORING CHAIN OF CUSTODY RECORD                                                                                        Page 1 ofl
 ~lient:    TVA                                                           Environmental Testing Solution, Inc.                                    Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery

Facility Sampled: Sequoyah NP 28801 General Commen~ Chevy Williams: ~ )._

L ~~

NPDES Number: TN0026450 Phone: 828-350-9364 Roy Quinn J~~.~-- Fax: 828-350-9368 Dissolved Metals Collected. Samples remained on ice Collected By: Chevy Williams, Roy Quinn through out sampling and transport to Jab. Field Identification I Sample Description Grab/Comp. Collection Date/Time Container Number& Flow MGD Rain Event? P!WJ O-J \YX)ss Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-

'C\

Inches Number Temp.

,\

ance (OC) SQN-101-TOX Comp 10/28/08-10/29/08 0809- 2 (2.5gal) 1551- X 0709 1558 AVG= C(JII}U\Oi Q.. oji.6'C o\ \,_,,_? 1555 SQN-INT-TOX Comp 10/28/08-10/29/08 0826-0726 I (2.5 gal) NA X 00 1\)"Zt\. uZ o.-0'v

                                                                                                                                                                                               ?}       \~1..?       +-

Sample Custody - Fill In From Top Down -i ~-\o~ ~ ~Jo..d. ~ Relinquished By (Signature): Date/Time Received By (Signature):

                                                                                                                                                                             . a..r- n ueol~ '-""-

_....,._.. rf . C(.. -*"'

d~

Date 1me 00 nl Ja-.. lit. oCJ5t EDI 0Cf51 6/JT CJuvJfJ~ Chevy Williams 10/29/08 Sonic Delivery 10/29/08

                                                                                                                 ,r,;".Jj
                                                                                                                 ~.'/f
                                                                                                                          -1.  -;,(__   ~

Sonic Delivery

10/29/08 1<-\?.~ ~T ETS cl'\ 1AA. /{ .... - ........ 10/29/08 I'-\ 1.3 E\ (~ ~

                                                                                                                   \.._)          I
                /

Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

~
~

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1

 ~
 ~Client:     TV A                                                         Environmental Testing Solution, Inc.                             Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Some Delivery Facility Sampled: Sequoyah NP 28801 General Commenr ~ Chevy Williams: J f J /J/; fJA. JI!J1 /

                                                                                                                                                                 ~ *_,..-~ . ~ t:)._. eo-NPDES Number: TN0026450                                                   Phone:          828-350-9364                                   Roy Quinn
                                                                                                                                                                 /V Fax:            828-350-9368                                   Dissolved Metals Collected. Samples remained on ice Collected By: Chevy Williams, Roy Quinn through out sampling and transport to lab.

Field Identification I Grab/Comp. Collection Date/Time Container Flow p/V.)j ed*U: '5L>st3 Sample Description Number& MGD Rain Event? Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-(EDT) -*- ........ Inches Number Temp. anee (oC) E:\ SQN-101-TOX Comp 10/30/08-10/31108 0802- 2 (2.5gal) 1551- X 0702 1556 00lb3!.0) /ojJec J- \0~,__. ~ AVG= 1554 SQN-INT-TOX 0834-Comp 10/30/08-10/31/08 I (2.5 gal) NA X (X3lD3IOZ.. /r & 'C a\ \~"?,; 0734 Sample Custody -Fill In From Top Down *- c.u...rto~ ~ ~o..d. ~ Relinquished By (Signature): Date/Time Received By (Signature):

                                                                                                                                                                                -~ Dater:J~?Ct Time (9 IT c) I~
                           ~~

Chevy Williams 10/31/08 IDOtGD(

Sonic Delivery 10/31108 loo/8{}-f

                                                                                                     ~                             ~

Sonic Delivery 10/31/08 10/31/08 r

  /:11                                                                            \ :,cs ?_ £ \      ETS                 It-r *.1\.lt.M"L.~                                                        \ '&~ (_     E.-,
                                                                                                             \._)       I
      ~ ...~L,~

J'-"1 :::>"'1 -It_~ (/ Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

I Page: )T6 I. Chron ._\'\;holt: Effhent !'oxicity Test ;£PA-821-R-O:>l13 \lethod lUOO.!J Species: Pimepfwles promt!lils I Client: TVA Facility: Seguoyah ~ uclear Plant NPDES #: TN 0026450 County: Hamilton Treatment: Non-treated Outfall: 101 I Project #: __,S""'C§>,._,.__.._K_ _ _ _ __ Dilution preparation information: Comments: l Dilution prep (%) Effluent volume (mL) Diluent volume (mL) 11.3 282.5 2217.5 22.6 565 1935 45.2 1130 1370 72.6 1815 685 100 2500 0 Total volume (mL) 2500 2500 2500 2500 2500 ll Test organism information: Test information: LA...._.,.~ Organism age: 2."\.~ t\a~ts 0\..t:) Randomizing template: I Date and times organisms were born between: 1~* '2.1* ocf t\oCO Incubator number: 3C.. Organism source: P..~C'f.. t, "-\C..~ C(p I C), 1.."\* 0 & Artemia lot number: e:fs-ICcHW oc I Transfer bowl information: Average transfer volume: pH= l.i1 Temperature= 2'-{.c.( Total drying time: -z.l.\*1-\olll..! Date I Time in: \\ *IJ'-'-ot l\o 2.0 Date I Time out: \\-<lS-tt \\:,1..0 1.8~ I_, Oven temperature: ~ (} :L_ Daily feeding and renewal information: I Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time batch used used I 0 time I 2 3 I 4 5 6 [ 7 I Control in.[ormation: Acceptance criteria Summary of test endpoints:

                 % Mortality:                                           o1.                        :-;20%                7-day LC 50          ) l CCI.,.

Average weight per initial larvae: I t"J.I.&[ *A:::~,~ ::.~::~~:i":_;~g_;m:;¥:'aHt£ NOEC JC01. Average weight per surviving larvae: £Lie &5 > 0.25 mg/larvae LOEC >10()7 .. ChV >'oc7. IC2s >I 00 7o 1 Page 26 of 96

I I J Species: Pimephales promelas Date: _i._.D..__*_,-z.....,.f'-*=cJ;:o___ __ I Client: TV A I Sequoyah Nuclear Plant- Non-treated Survival and Growth Data I Day A CONTROL B c D E F 11.3% G H I J 22.6% K L 0 to to I 1

                                               /0               10               IO        IO       IO        IO
                                                                                                                       /0 IO IO I{)
                                                                                                                                                  /0          10 It         ID      ID     10       /()        ID        10      I 0                                  10          10 I                           2 3

IC /(J ID /0 /0 /0 IO IO 10 IO 10 /0 10 lo /O 10 (b 10 10 /0 /0 /0 10 JO I 4 10 10 IO /0 /0 /0 /C 16 {() IO /0 IO 5 IO I 6

                                              /0        f(J      /6      10      /0         IO      IO       IO          I()            /Q        10 10         /Q      IO      /0        IO       10       10      /D          I(J             10        ID 10 7

ID It> 10 /0 IO IO /0 /0 to 10 IO 10 A = Pan weight (mg) Color identification: bla.d.<. 1'1.~0 \~.45 IS.OI 1~.'\1. t~.3S 1'-!.18 lt.80 \5.1'l 1~.1.1 IS.SI 15.0\o IS.S(p Analyst: LA-A B =Pan+ Larvae weight (mg) Analyst: '{\,)L~

                                           ?. 1.'-\l -z..\.00 z:z.l5 L.\ .t?~ D .Z.k 7-t.\O 10.'11         7_21Z 20.41 L."ZJ,c\:; Zt.l~                     zz.ob Larvae weight (mg) =A - B 7.Dl (;55 /,ll (p, 17. Sctl                 (o_qz.     ~.01 (,. G3 ~(p~ 7.01 ~13                                 ~.50 Weight per initial number lo.1n                    p.(.,C\1... o.i]\ \ D*i.oct'l IO*~I,;'-1 o.l-11 0.\o\3                    ;D of larvae (mg)
          = C I Initial number of larvae t;.16l D.~S                O."'TZ.. c.C~/\\                                                                     \,)

Average Percent weight per reduction initial from control (). b && o.lolo'"1 ,3.\1.. o.to-5'1 £.\. '27. number of (%) larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Calculations and data reviewed: ~ Comments: Page 27 of ll

Sptcies: Pimi!ph,z!c>s promt'fas Client: TVA. Sequoyah :\udear Plan;- _';on-rreated Survival and Growth Data Day 45.2% 72.6% 100% M N 0 I p Q R s T u v w X 0 to ID 10 /0 10 /0 IO /0 10 10 /0 ID 1 ID ID /0 /{) /D IO /() /{) 10 I() /D /(J I 2 JD /0 ID 10 /0 10 tO /0 IO 10 tO 10 3 co I 4

                                           /0      10         10        10       10        10       /D     /()      10 10       ID 10 iD 10 to          ID       /C         10      10       lC       I{)       /0                                10 I                         5
                                            /{)         to     /D      /()        to ID              /0     ID       /() 10          10          /0 6
                                            /0        /()      10       10        to       /C      10       /(J     /0       10      /()      'i.e~\~ :r I                         7 10         ID         "***

10 10 /0 to to fC ILfr

                                                                                                                    /()      IO      {()       '1 A= Pan weight (mg)

I Color identification: blOcK Analyst: L&0 1~52. (!.{. '01 1~.1~ 13.U. 1~.18 ~ (3.31 r{o'-# \3.\lt.f 1'1.'13 11\.1.1. \5.~\c t~. ~~ B =Pan+ Larvae weight (mg) Analyst: ~L~ ZO.'S"t; -z.l.ll ?.:z .3lo zo.(J) -,0~ u .u \Cj .5~ lct.B\ -zof:J~ C:l.l~ lq. ~~ Z:i_i:SI (0.\'Z. Larvae weight (mg) =A- B S,blo l,o. jO 1.loo \v.bb lv.~1 l,.\l S.ll I 7.'2~ ~.35 5J,5 Vt5 '5.03 Weight per initial number

                                                                                                                  ~~5
                                                                         ,.,a_

of larvae (mg)

                                        ----tS~  ().

IJ!r;o o.-1\ilo 0 .w'O" o\D rJ-1 r IJ.

                                                                                            \o\l ~5-n c.-'lz.L.\

0.96 t.iol\5 o~'13

         = C I Initial number of larvae V*

o.v Average Percent weight per reduction initial from control o.t.,Cob 3. ?7. O.I..'*N \..Y 1. O.lo\(.) l\.l.H'. number of (%) larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: ~ Comments: Page28of9~------------------------------------------------------------

I I I ~pedes: Pin-It!phaJ.::s promr:las Client: TVA_, Sequoyah \udear Pl:am - ~;on-treate*! D:.ne: ------- n~*u-o& ----~--- I Day Survival and Growth Data 100% Intake y ! z I 0

                                                                             /o      /0 AA ID BB
                                                                                                             /0 I

I 2 I~ IO IO 10 J() 10 /0 ID I 3 10 lb 10 10 4

                                                                             /0       10        ro         IC I                                                           5 10        IO         't ItA. F'Er IC I                                                          6 7

IO IO g'cJ.Fl r 10 H .._.. g\L.b ~,AF I~ IC I A= Pan weight (mg} Color identification: lolQc.Jc 63Y 14.10 13.C0t 1~.ot1 Analyst: 1~- I B =Pan+ Larvae weight (mg) Analyst: bL_) LL.,\3 1_.(,,'t~ lCt!jO zo.t.~ I Larvae weight (mg) =A- B s.ift vz-z G~~ l.S'b Weight per initial number of larvae (mg) o.lo_,~ o.1.5i6 0~~ Q. Ill-z 'L

                                          = C I Initial number of larvae Average            Percent weight per         reduction initial            from control   o .~eo s1              4.'57.

number of (%) larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Calculations and data reviewed: e}r-Comments: Page 29 of96

   "tt                                                                               TVA I Sequoyah Nuclear Plant, Outfall101- Non-treated
   ~
 ~
   ~

October 28- November 04,2008 w 0 0

Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses

.;;iif.7 Environmental Testing Solutions, Inc.

Not for Compliance Assessment, lnternal Laboratory QC Project muHbt*.,*. f*** t v" ' T~~~llif

                                                                                                                                                                                                                                                                                                           --- *-- -~ -----

SO:-.~! Concentration(%) Replica~.tc Initial oumberof Finlll number of A"' Pu weight B"' Pan + Larv~ac Lan*ac weight (mg) Weight I Sunh*ing Men lteigbt/ Coefficient ofnri~tion Weit:,bt/loitial Dumber Mean sun*i'"' Mcll.nncil!hl/ Pcn:tut n:tlunion from harvae larvae (mg) \o\oeigb1 (mg} ~A-B .a umber uflarvae (mg) Surviving ouml~r nf (Muaw-clthl , ... .,.rvl~h*c uf lan'llC (mg) (%) lnitillloumhcr ur \llflill1ill\' _.1,, I ,,,,Hoi(%) IJtn*ac (m£) ou*b"' ~flanu){ 0/*) tunuc (Ill!!) 1-' '"'"~I'""" A 10 10 14.40 2!.47 7 07 0.707 0 707

                                                                                                                                                                                                                                                                              ---~              -+------

B 10 10 14 45 21.00 6.55 0.655 0 655

                                                                                                                                                                                                                                                                                                 .-1.-~:_"""lirablc Control                                                                                                                                           0.688                  4.2                                            100.0             0.688                           -Ll c               10                 10             15.01               22.18             7 17                0 717                                                                     0 717 D               10                 10             14 92              21 64              672                  0.672                                                                    0 672
                                                                                                                                                                                                                                                               ... +---*-********

11.3% E F G ll 10 10 10 10 10 10 10 10 14.35 14 18 12.80 15 79 20.26 21.10 19 71 22 72 5 91 692 6 91 6 93 0 591 0 692 0 691 0 693 0 667 76 0 59! 0.692 0 691 0 693 100.0 0.667 i 1.6 l I J! J [ 10 10 10 10 14 27 15.57 20.91 22.66 6.64 7 09 0.664 0 709 0 664 0 709

                                                                                                                                                                                                                                                                             ~------*
                                                                                                                                                                                                                                                                                                -T 22.6%                                                                                                                                             0 659                  60                                             100.0              0.659                          [I()                             *. 2 K               10                  10            15 06               21.19             613                  0 613                                                                    0 613 L               10                  \0            I 5 56              22 06             6 50                 0.650                                                                    0 650 M               10                 10             14.52               20.38             5 86                 0 586                                                                    0.586 N               10                  10            14.87               21.17             6.30                 0.630                                                                    0.630 45.1%                                                                                                                                            0.666                  11.3                                           100.0              0.666                                                           J.2 0               10                 10             14.76               22.36             7.60                 0.760                                                                    0.760 p               10                  10            13.72               20.60             6 88                 0.688                                                                    0.688 0.657 1--*---t---------1 Q               10                  10            1428                20.85             6 57                 0 657 R               10                  10             13.37               19.54            6.17                 0 617                                                                    0.617 72.6%

T u s 10 10 10 10 10 10 14 04 13.64 14 93 19 81 20.88 21 28 5.77 7.24 6.35 0.577 0 724 0 635 0.644 9.7 0.577 0.724 0 635 100.0 0.6~~ L ___. . . 6.4 100% v 10 10 14.22 19.87 5.65 0.565 0 626 67 0 565 97.5 0.610 w X y 10 l0 10 10 9 10 1536 14 19 15 34 21.81 20.12 22 13 6.45 5 93 6.79 0.645 0.659 0.679 0 645 0 593 0.679

                                                                                                                                                                                                                                                                                                  -f------11. --

100°/o Intake z 10 10 14 70 22.28 7.58 0.758 0.710 49 0 758 92.5 0.657 1.:. -LS AA BB 10 10 8 9 13.81 14 04 19.50 20 26 5 69 622 0 711 0 691 0.569 0 622 _.-l . . ____ Outfall101: MSD~ Minimum Significant Difference Dunnett's MSD value: 0,0879 PMSD~ Percent Mmimum Sign1ficant D1fference PMSD: 12.8 PMSD is a measure of test precision. The PMSD is the minimum pe1cent difference between the control and treatment that can be dt!clared stat1.sllc~!ly slg!l!li . . ,JJ)I i11 11 whok . .*ll)\1, 1l! tdxlct!y te:st lnt11.ke: Dunnett's MSD value: 0.0836 Lower PMSD bound determined by USEPA (101h percen1ile) ~ 12%. PMSD: 12.Z Upper PMSD bound de1ermined by USEPA (90th percenl!le) ~ 30%. Lower and upper PMSD bounds were de1ermined from the I Oth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Va11abdoty StuJy (liSLI',\ 'IHJ 1"* I I'U'A, cOO I b) USEPA 200 I a, 2001 b. Finai Report: Interlaboratory Variabthty Study of EPA Short-term Chronic and Acute Whole Effluent Toxic1ty Test Methods, Volumes 1 and 2-Appendix.' EPA-821-8-0 1-004 and EPA-821-B-Ol-005. US EnvJronnh:!ntul JlrutcCltun -"\~'-"n~o.y, l'int:llllh-111, OH

1-C ~ TVA I Sequoyah Nuclear Plant, OutfalllOl- Non-treated ~ ~ w October 28 - November 04, 2008 0 \0 0\ Statistical Analyses Environmental Testing Solutions, inc. Larval Fish Growth and Survival Test-7 Day Growth Start Date: I 0/28/2008 Test ID: PpFRCR Sample ID: TVA I Sequoyah Nuclear Plant, Outfall I0 I End Date: 11/4/2008 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Cone-% I 2 3 4

                                         -==-:-::-:c-:----":-=::c:-----:--:-=-:-:---------------------------------***--*-----

D-Control 0.7070 0.6550 0.7170 0.6720 1!.3 0.5910 0.6920 0.6910 0.6930 22.6 0.6640 0.7090 0.6130 0.6500 45.2 0.5860 0.6300 0.7600 0.6880 72.6 0.6570 0.6170 0.5770 0.7240 100 0.6350 0.5650 0.6450 0.5930

                                                                                                                                                                             --*---~    ----

Transform: Untransformed !-Tailed Lsotuni,* Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Meiln N-Mcan D-Control 0.6878 1.0000 0.6878 0.6550 0.7170 4.236 4 0.6878 1.0000 1!.3 0.6668 0.9695 0.6668 0.5910 0.6930 7.575 4 0.576 2.410 0.0879 0.661,X ll. '/()'j') 22.6 0.6590 0.9582 0.6590 0.6130 0.7090 6.020 4 0.788 2.410 0.0879 0.6025 O.LJ(>i.i 45.2 0.6660 0.9684 0.6660 0.5860 0.7600 11.308 4 0.596 2.410 0.0879 0.6625 1).%.\j 72.6 0.6438 0.9360 0.6438 0.5770 0.7240 9.737 4 1.206 2.410 0.0879 0.6438 ll.LJ.\(>0 100 0.6095 0.8862 0.6095 0.5650 0.6450 6.112 4 2.145 2.410 0.0879 0.6095 0.!!81>2 Auxiliary Tests Statistic Critical Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.9664126 0.884 Bartlett's Test indicates equal variances (p = 0.67) 3.22156215 15.0862722 Hypothesis Test (l-tail, 0.05) NOEC LOEC ChV TU MSDu MS Do MSD MSE Dunnett's Test 100 >100 1 0.08789734 0.12780421 0.00283454 0.0026604 Treatments vs D-Control 58.553 92.420

                               >100
                               >100
                                >100
                                >100
                                >100 sqnl (!I I 0-28.-UI:idaw

'"d I:W TVA I Sequoyah Nuclear Plant, Intake- Non-treated ~ ~ w October 28- November 04,2008 N c 1.0 0\ Statistical Analyses

     \
     ...J Environmental Testing Solutions, Inc.

Larval Fish Growth and Survival Test-7 Day Growth Start Date: 10/28/2008 Test ID: PpFRCR Sample ID: TV A I Sequoyah Nuclear Plant, Outfall I 0 I l~ttal-.c End Date: 11/4/2008 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-0 13 Test Species: PP-Pimephales promelas Comments: Non-treated Cone-% 1 2 3 4 D-Control 0.7070 0.6550 0.7170 0.6720 100 0.6790 0.7580 0.5690 0.6220 Transform: Untransformed !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-*Mcdll D-Control 0.6878 1.0000 0.6878 0.6550 0.7170 4.236 4 0.6R78 1.0000 100 0.6570 0.9553 0.6570 0.5690 0.7580 12.320 4 0.715 1.943 0.0836 0.6570 0.'.15:\.i Auxiliary Tests Statistic Critical Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96301621 0.749 F-Test indicates equal variances (p = 0.13) 7.71728659 47.4672279 Hypothesis Test (!-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.08358076 0.12152782 0.00189112 0.00370012 0.50 15206]*---**----- 1. (> Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 95% CL(Exp) Skew 1C05 >100 IClO >100 IC15 >100 JC20 >100 IC25 >100 IC40 >100 ICSO >100 sqn!IJ/ _lO .'.ll OHdo!a

  "d
  ~

TVA I Sequoyah Nuclear Plant, OutfalllOl -Non-treated (TQ ('!) October 28 -November 04, 2008

.ww 0                                               Pimephales promelas Chronic Whole Effluent Toxicity Test
~
~

EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses '<) Environmental Testing Solutions, Inc. Project nuwhrr: 5058 Reviewed h); (-""--l£c.4.- * " ' - - Concentration Control 11.3% 22.6% 45.2% 72.6% 100% 100% Intake

Spece~: Pimeph,tf.:, .,.,Jinei!IS Cii~m: TVA S.::qu.;:c;h \ude:.H :)!ant-- **Jn-'rt'Jted 11.3% 22.6% 45.2% 72.6% 100% 100% Intake Page 34 of 96 1

I I Spec:,;:-;: F:m"_*;lzaft'\ .).*;J*:"*ws Clien. TV.-\ . Seqc;. *. , h *, Jclear ?:dGt - *~,;n-rrc:.J ;;d I I Concen- Parameter tration I I I I 11.3% Jt- c ~t.)t.\)c;:t'Nm .., "' ......,.,..,.

                                                                          - A .......~~             tte. A.N "'-'11:Cl:> .... "A.Ult~ ~ette. (.ONC:'\~1"\et::>.
                                                                                       '!';Ri'U~.

P1ge 1 of7 Chron.ic \\hole Effluent Toxicity Tesr tEPA-811-R-02-1)13 \IethoJ 1002 OJ Species: Caiodaphnia dubia Client: TVA County: Hamilton Facility: Seguovah Nuclear Plant Treatment: Non-treated I NPDES #: TN 0026450 Project#: -~=Oc.::S=--=2,___ _ _ __ Outfall: 101 I Dilution preparation information: Dilution prep (%) 113 22.6 45.2 72.6 100 Comments: Effluent volume (mL) 282.5 565 1130 1815 2500 I Diluent volume (mL) Total volume (mL) 2217.5 2500 1935 2500 1370 2500 685 2500 0 2500 Test organism source information: Test information: Organism age < 24-hours old Randomizing template color* &...flP() ..._, Date and times organisms were born \t!*21*08 115& T"' 1\o\'-\ Incubator number and shelf between: location: 2'&\ Culture board tt~*t\*Qf A '1)*1. \- 646"1. Replicate number: lj2jlj4j5j6j7j8 YWT batch: 9 ' Jil' IO*O'o-0~ Culture board cup number: . I

                                            ~    -s               I     I I 1 /l"l. '~ '8 1*n I 'lR' ~1.1lb o

oc Transfer bowl information: pH= 1."1'-\ su Temperature = 1.'l .\ Selenastrum batch: ~*1.t\*~ Daily renewal information: Day Date Test initiation, Control water Sample numbers Analyst batch used used Ml\~~ 0 2 3 4 5 6 7 Control information: 1 2 Acceptance criteria Summary of test endp_oints:

            %of Male Adults:                          o7..        01.                 ~20%                  7-day LCSO            )10<)7.
            %Adults having 3'd Broods:               Joo1.       lex/f..              ~  80%                NOEC                     1()0 7,
            %Mortality:                               D1.           D1..              ~20%                  LOEC                    )1001..

Mean Offspring/Female: J~.~ 31._.~ ~ L5.0 offspring/female ChV 'l IOQ 1.

            %CV:                                     ....1..1.    ~.o.ct .           <40.0%                 IC25                  >1007.

Page 36 of96

  ~~~ftS-~

1'):'; ":' ...

~

I I Spe~ies: Ceriodaphni b a () \\o 1\o Adult mortality L \._ \..._ \._ \._ L L \._ L L

' 7 Young produced \~ D l\ ~0 \S I--\ 1\ 1\ () 0 I Total young produced -z.~ 35 ~c,( ~ ~~ 3\ 33 ~s 03 ~~
Final Adult Mortality \.._ '-- '- '- \._ \._ L. '- '- '-
-,c._
I
-;c... ><:.
X for J'd Broods ~ ~ ~ ~ >'- ~ ~ Note: Adult mortal tty (L = l!ve, D = dead) Concentration: I Survival and Reproduction Data CONC: 11.3% I Day 1 Young produced 1 0 2 0 3 0 0 4 Replicate number 0 5 0 6 () 7 0 8 0 9 0 10 Adult mortality L L '- L L L L '-- \...._ \._ I 2 Young produced Adult mortality 0 L L 0 L 0 L 0 0 L 0 L 0 L a L 0 L 0
\......
3 Young produced 0 0 0 0 0 0 0 0 0 0 I 4 Adult mortality Young produced L-l.\ L
\o L
s s
\..-
s
\...... L '-I L .s L
s L
~
l_ L\ Adult mortality L L L L- \_ L L \._ \_ I
\...
5 Young produced 1'-\ n. . ~~ t4 I"L \1... l'-\ \~ t'-\ 1"2._ Adult mortality L L L L L L 0 L 0 L L \._
'"' 0 6 Young produced \\.p l\
I Adult mortality a L L 0 L L Q_ L 0 L
\J L L I (a 0 L
1$ 0 l D
*~
7 Young produced \\a l<i I,... Total young produced Final Adult Mortality
~ \.._
3S L-
~'-\
3!
~'5 \.._
3\ L
~1 3'-\
L 31
\.._ ~\
L Note Adult mortality (L = hve, D =dead) I Concentration:
% Mortality: (J7.
Mean Offspring/Female: ..3 '1 . .,. rPage 37 of 96 % Reduction from Control: - .3.<<t 1.
I I Snecie~: Cc"iudaphnia :luhia I 1 Cliern: Seguov~:th \;uclear Plant- "<on<reated CO"iC 22.6% Survival and Reproduction Data I Dav 1 0 0 2 I J C::> J I ~ 0 Replicate number 5 01 (51 6 0 7 I 8 0 0 9 i 10 1 Young produced 0 L L L L_ L '- '- \........ I Adult mortality L L 2 Young produced a 0 [) () 0 (") 0 0 0 0 Adult mortality L L '-- L '-- '- L L L \..__ 0 I 0 0 0 0 3 Young produced 0 0 () 0 0 Adult mortality L L L \.._... L L L '- L. L. 4 Young produced 4 ~ 4 \o \...\ s \.o 5 s '-\
\.._. L L '- '-- L I L. L Adult mortality L. \.._.
5 Young produced \'S '~ l.=, \~ l"-\ - lS I~ \3, Adult mortality L- \.. L '-- \._ \.._ L
'- L \.._
1S I 6 Young produced Adult mortality
\.8 L \'1 L \\ \..._ \\
L. 0 l8
\_ \(\
L. D L 1\
\..........
7 Young produced 0 0 0 0 0 llo 0 0 () I Total young produced Final Adult Mortality
~., '-- L ~g ~"' \.._ ~ ~I,.
L
.3\o L
3"\
\......
3S .3\
'- L ~\
Note: Adult mortality (L =live, D =dead) I Concentration:
%Mortality: (j7 Mean OffsprinldFema1e: 3S*1 I CONC: 45.2% %Reduction from Control:
Survival and Reproduction Data _,. 7.1. Rtolicate number I Day l Young produced a 1 0 2 0 3 0 4 5 CJ 0 6 0 7 8 0 0 9 () 10 Adult mortality \..._ L '- '- '-- L '- '- '- '-- I 2 Young produced Adult mortality n L a
\,_..
() L. L 0 L () (\ L. 0 L. 0 L- L 0 L a 3 Young produced 0 0 0 0 () 0 0 0 0 0 I 4 Adult mortality Young produced L. c...\ 5 L s L L s L
~ \.._ '5 L \..._ '--\
L L\ Adult mortality L L L L L L L L L \._ I 5 Young produced Adult mortality
\~ \._ *~ \..._.
l~ L IS L 1'2.. L
~
I~
\..... L 1,3 II..\
L '- 13 6 Young produced \$ l\ 0 lq 0 l& l\o \'\ l'\ 1'\ I 7 Adult mortality Young produced 0
\.._ L-0 L-l'\ 0 L ,, L-0 \__
0 L.. 0 L 0 L L 0
~
Total young produced
32. 211 ~ 3'-l 3Co ~s 3'1 ~, ~
Final Adult Mortality L L '-- '- L '- --c.= \.... '-- L Note: Adult mortality (L =live, D =dead) Concentration:
% Mortality: 07.
Mean Offspring/Female: .,31o. 3
%Reduction from Control: - q, o7.
Page 38 of96
Specie c;: Caiodaphnia dubia Client. Sequovah 'luclear Plant* :<w-tre n~d Survival and Reproduction Da! IS Adult mortality L L L \._ \._ \..... L L \._ L 6 Young produced
\i L.O tt\ 0 \\ \\ \'\ () "1.0 \&
Adult mortality L_ \..._ \._ L L L L '- L '- 7 Young produced 0 0 0 \'\ 0 0 0 \\ 0 0 Total young produced
~~ YD 3~ 3\ ~'-\ 3'5. 3c; 3\o 3t 3~
Final Adult Mortality '-- L '- L \._
'-- '- L L \.....
. Note: Adult mortality (L = live, D = dead) Concentration:
% Mortality: O"t.
Mean Offspring/Female: .31- ...\
% Reduction from Control: -\2. 3"1.
CONC: 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced 0 0 0 0 6 1'1 0 () () 0 Adult mortality L L L \._ L \....... \._ L L L 2 Young produced a D () 0 0 0 0 0 0 0 Adult mortality L L L L L L- L L L L._ 3 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L L L L L \_ L L \.._ 4 Young produced \.o '-\ b -~ s s \.::, s .s lo Adult mortality L \._
'- L t_ L L L L \.
lL\ 1"-\ \~ \~ l~ l'-\ 14 1$
*~
5 Young produced l\o Adult mortality L L L L L L L L L L l'\ l~
'" l~ L.\
6 Young produced 2.\ 1...0 D 'Z..O "2..0 Adult mortality L L \._ L L_ L._ \...._ L L L 7 Young produced 0 0 0 0 \8 0 0 0 0 0 Total young produced
<..{~ .31 3'\ 31 ~g. ~&. 41.. 3'\ 31 ~L Final Adult Mortality L L \,__ L L L L L L '--
Note: Adult mortality (L =live, D =dead) Concentration:
% Mortality: 0?.
Mean Offspring/Female: ..3 ~- 2.
%Reduction from Control: - n :1'7.
5 pecies: Ccriadap ,'z Iia dubia Client: SeguO'* 1h ,, uL!ear ?!ant **'\on-treated CO'iTROL-2 Survival and Ri!production Data Replicate number Dav I 2 3 .t 5 6 7 i 8 I 9 i 10 1 Young produced 0 0 0 0 0 c 0 0 6 c Adult mortality L L L L '- L L. L L L_ 2 Young produced D 0 0 0 a 0 0 0 0 0 Adult mortality L L L L L L \..._
'- L '--
3 Young produced 0 0 0 0 0 0 0 0 Q 0 Adult mortality
'-- '- L L L.. '- L '- '-- '--
4 Young produced ::s 5 '-\ '-\ "-\ $ '-\ 1..\. \o '-\ Adult mortality L \.._ \.._ '- '-- L '- '- \._. \_ 5 Young produced I\ L4 \"2.. \~ n_ \1_ \\ \~ \~ \ \ Adult mortality \._ L L L. \_ L
'-- \..__ '-- \......
6 Young produced II.\ 0 0 0 0 0 0 0 a - Adult mortality L L. '- L L L '-- L \..... \._. 7 Young produced _D \\ \'-\ no \\o l'lo llo l"-'. \~ 0 Total young produced
~0 3\.o ~0 3~ 3'Z. 3~ ~\ o1.... 3'\ ~\
Final Adult Mortality \....- L '-- L L \.._ '- '- '-- '--- Note: Adult mortality (L =live, D =dead) Concentration:
% Mortality: I D.,.
Mean Off~ring!Female: I .32._~- coNe: 100% Intake Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 D D 0 0 a 0 0 D 0 Adult mortality L \_ L.. \_ L L L L L L 2 Young produced (") 0 0 0 a D a a_ 0 0 Adult mortality
'- L L L L L L L L '---
3 Young produced 0 0 0 0 (') 0 0 c 0 0 Adult mortality L L L L \..._.
'-- L '- L \.,_
4 Young produced s '-\ y 4 s s q '-\ s '-\ Adult mortality L L L. L L L L L L L 5 Young produced Adult mortality
\2. "-L \\ \._
l~ L
\1- '-- L \"1...
L
\£... \D '~L \ '"Z....
L. 6 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L_ L L L L_ L- L-L L-7 Young produced
'~ \'5 \t\ \'\ \lo \S 18 \S \\ tS Total young produced 3'5 ~\ 3'-' 3lo 3~ 3~ ~4 "Z.'\ ~~ 3\
Final Adult Mortality L '-- '-- L '--- L L L. L Note: Adult mortality (L =live, D =dead) Concentration:
% Mortality: (17.
Mean Offspring/Female: -~~.() I Page 40 of 96 %Reduction from Control:
2.. 'l1.
""tj ~
TVA I Sequoyah Nuclear Plant, OutfalllOl (1'1
~ October 28- November 04,2008 ~
Verification of Ceriodaphnia Reproduction Totals 0
'.C ~'- Envlronm@ntal Tasting Solutions, Inc.
0'\ Control-1 72.6% Replicate number Replicate number Day I 2 3 4 5 6 7 8 9 10 Total Day 1 2 3 4 5 6 7 8 9 10- Total j l 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 I) () I 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 () -0 () () I
-- j 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 () 0 1--- -**
()
~ .. -~
IJ 4 4 6 5 4 6 4 4 4 5 5 47 4 6 4 5 5 5 6 5 5 5 0\ Tl
~
I 5 11 13 14 13 13 13 12 14 12 12 127 5 14 16 IS 13 12 12 15 14 l:i ll<J 6 0 16 0 17 0 0 0 0 16 16 65 6 18 20 19 () 17 17 19 0 20 IH I:IX 7 14 0 17 0 15 14 17 17 0 0 94 7 0 0 0 19 0 0 0 17 0 0 3o ! Total 29 35 36 34 34 3I 33 35 33 33 333 I Total 38 40 39 37 34 35 39 36 3R 38 37-1 11.3% 100% Replicate number I Replicate number .. I ' Day Total Day Iota I 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 () II 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 ' 0 0 0 0 0 0 0 () II 3 0 () 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 I) I! 4 4 6 5 5 5 4 5 5 6 4 49 4 6 4 6 4 5 5 6 5 5 6 S2 5 14 12 13 14 12 12 14 13 14 12 130 5 16 14 14 13 15 14 16 14 14 l'i II-i 6 16 17 0 19 0 0 0 0 17 15 84 6 21 19 19 20 0 19 20 20 18 t--- 21 177 7 0 0 16 0 18 15 18 16 0 0 83 7 0 0 0 0 18 0 0 0 () () IX Total 34 35 34 38 35 3I 37 34 37 31 346 Total 43 37 39 _37 38 38 42 39 37 42 392 22.6% Control-2 Replicate number I Replicate number Day Total Day l dial 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 l 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 ,(\ :I -"- 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 () I} () II r---*-- 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 () 0 () 0
--~----~-~
() 4 4 6 4 6 4 5 6 5 5 4 49 4 5 5 4 4 4 5 4 4 6 4 ~'i 5 IS 13 13 13 14 12 I5 13 13 12 133 5 11 14 12 14 12 12 II 14 13 II I ;:J 6 18 19 17 17 0 19 0 17 19 I5 141 6 14 0 0 0 0 0 0 0 0 Ill 30 7 0 0 0 0 18 0 16 0 0 0 34 7 0 17 14 16 16 16 16 14 15 (I [7.) Total 37 38 34 36 36 36 37 35 37 31 357 Total 30 36 30 34 32 33 3I 32 34 31 32.1 45.2% 100% Intake Re licate number Replicate number Day Total Day 9--r---w 'fulal I 2 3 4 5 6 7 8 9 IO 1 2 3 4 5 6 7 8 I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 () II 0-- ) --0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 *-- () 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 () () 4 4 5 5 5 5 4 5 7 4 4 48 4 5 4 4 4 5 5- 4 4 5 .:J 11 5 13 16 13 15 12 14 14 13 14 13 137 5 !? 12 11 13 12 12 12 10 1.1 I'J II'!
*------(I ---**
6 15 17 0 19 0 18 16 19 19 19 142 6 0 0 0 0 0 0 0 0 0 0 ...... - - --- 7 0 0 19 0 17 0 0 0 0 0 36 7 18 15 19 19 16 15 18 J5 li- 1) 167 Total 32 38 37 39 34 36 35 39 37 36 363 Total 35_ 3I 34 _36 33 32 34 29 35 31 .no
'"C ~
TVA I Sequoyah Nuclear Plant, OutfalllOl
~
('> October 28- November 04,2008 N 0
~ Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test ~ EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc.
Project number: -~ -T__~-- --- -- 5058 t - t .+'- - Concentration Replicate number Survival Average reproduction T oeffirl£:11 t of Pcrcclil reduction frtlHI
\:anatiu11 ('~, JnHdul cuutrub CX,}
(%) (%) (offspring/female) 1 2 3 4 5 6 7 8 9 10 Control- 1 29 35 36 34 34 31 33 35 33 33 100 33.3 (,,2 Not applicable
--~--- **---
11.3% 34 35 34 38 35 31 37 34 37 31 100 34.6 6.8 -3.9 22.6% 37 38 34 36 36 36 37 35 37 31 100 35.7 :),(, -7.2 45.2% 32 38 37 39 34 36 35 39 37 36 100 36.3 6.1 -'1.0 72.6% 38 40 39 37 34 35 39 36 38 38 100 37.4 5.1 -12.3 100% 43 37 39 37 38 38 42 39 37 42 100 39.2 5.'1 -17.7 Control- 2 100% Intake 30 35 36 31 30 34 34 36 32 33 33 32 31 34 32 29 34 35 31 31 100 100 32.3 33.0
'*" :==L'"' .,,,,,,\<
(o.7
-2.2 Outfall101: MSD= Minimum Significant Difference Dunnett's MSD value: 2.195 PMSD= Percent Minimum Significant Difference PMSD: 6.6 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can he uecl,ucd ,,l<~listicallv significant in a whole effluent toxicity test.
Intake: Dunnett's MSD value: 1.615 Lower PMSD bound determined by USEPA (I Oth percentile) = 13%. PMSD: 5.0 Upper PMSD bound determined by USEPA (90th percentile)= 47%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively. ofPMSD data trom FPA's WFT lnkr/,tbor"llltY Variability Study (USEPA, 200la; USEPA, 200lb). USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US LiiVlllllllilcllial Prokct1on Agency, Cincinnati, OH. USEPA. 2001 a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-u 1-00-1 and L'P;\ >L' I -ll-0 1-005. liS Environmental Protection Agency, Cincinnati, OH.
~ ~ ~ r!) TVA I Sequoyah Nuclear Plant, OutfalllOl ~ October 28- November 04, 2008 ....0 \C 0'\ Statistical Analyses
) Environmental Testing Solutions, Inc.
CeriodaEhnia Survival and Reproduction Test-Reproduction Start Date: 10128/200& Test ID: CdFRCR Sample ID: TV A I Sequoyah Nuclear Plant, Outfall I 0 I End Date: 1114/2008 Lab JD: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-&21-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Cone-% I 2 3 4 5 6 7 8 9 10 D-Control 29.000 35.000 36.000 34.000 34.000 31.000 33.000 35.000 33.000 33.000 11.3 34.000 35.000 34.000 38.000 35.000 31.000 37.000 34.000 37.000 31.000 22.6 37.000 38.000 34.000 36.000 36.000 36.000 37.000 35.000 37.000 31.000 45.2 32.000 3&.000 37.000 39.000 34.000 36.000 35.000 39.000 37.000 36.000 72.6 3&.000 40.000 39.000 37.000 34.000 35.000 39.000 36.000 3&.000 38.000 100 43.000 37.000 39.000 37.000 3&.000 3&.000 42.000 39.000 37.000 42.000 Transfom1: Untransformed ------- J-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-rvlcC~JI D-Control 33.300 1.0000 33.300 29.000 36.000 6.179 10 36.08.1 I 0000 11.3 34.600 1.0390 34.600 31.000 38.000 6.839 10 -1.355 2.287 2.195 36.083 I 0000 22.6 35.700 1.0721 35.700 31.000 38.000 5.610 10 -2.501 2.287 2.195 36.08.1 I.OUOt) 45.2 36.300 1.0901 36.300 32.000 39.000 6.09& 10 -3.126 2.287 2.195 36.0H3 I.OUU!I 72.6 37.400 1.1231 37.400 34.000 40.000 5.073 10 -4.272 2.2&7 2.195 36.0H3 1.!)0()() 100 39.200 1.1772 39.200 37.000 43.000 5.&67 10 -6.147 2.287 2.195 36.083 l.UOOO Auxiliary Tests Statistic Critical Skew Kurl Kolmogorov D Test indicates normal distribution (p > 0.01) 0.66729027 1.035 -0.3926353 -0.1779355 Bartlett's Test indicates equal variances (p = 0.99) 0.62959719 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU *------*-* -~-- *-~ MSDu MSDE MSB MSE F-Prob dl' Dunnett's Test 100 >100 2.19461764 0.06590443 43.1766667 4.60555556 1.8E-06 5. 54 Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 95% CL Skew
!COS >100 JC10 >100 1CI5 >100 IC20 >100 lC25 >100 IC40 >100 JC50 >100 sqn/IJ/ j().Ji)..{)Krlola
"d ~ TVA I Sequoyah Nuclear Plant, Intake (!) October 28- November 04,2008 0 \0 Q'l Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reeroduction Start Date: 10/28/2008 Test ID: CdFRCR Sample ID: TVA I Sequoyah Nuclear Plant, Outfall I 0 I - lniak" End Date: 11/4/2008 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Cone-% 1 2 3 4 5 6 7 8 9 10 D-Control 30.000 36.000 30.000 34.000 32.000 33.000 31.000 32.000 34.000 31.000 100 35.000 31.000 34.000 36.000 33.000 32.000 34.000 29.000 35.000 31.000 Transform: Untransformed 1-Tailed holonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-M~'"' D-Control 32.300 1.0000 32.300 30.000 36.000 6.026 10 32.650 I lll)ill) 100 33.000 1.0217 33.000 29.000 36.000 6.700 10 -0.751 1.734 1.615 32.650 I IJI)IJ(I Auxiliary Tests Statistic Critical Skew Ktiil Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97574127 0.868 -0.0277877 -0.67-1,11(,5 F-Test indicates equal variances (p = 0.71) 1.29032254 6.54108953 Hypothesis Test ( 1-tail, 0.05) MSDu MSDp MSB MSE F-Prob ,_jj' Homoscedastic t Test indicates no significant differences 1.61535987 0.05001114 2.45 4.33888889 0.46210 I 0-1 I. I R Treatments vs D-Control Linear Interpolation (200 Resamples) -- ---*---*---*** Point % SD 95%CL Skew IC05 >100 IC10 >100 IC15 >100 IC20 >100 JC25 >100 IC40 >100 1C50 >100 si.fniO/ /{I .:s IJSclula
"'C TVA I Sequoyah Nuclear Plant, OutfalllOl - Non-treated ~
(JCI (!) October 28- November 04,2008 .!Jl
~
0 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test IIG ~ EPA-821-R-02-013, Method 1002.0 Daily ChemicalAnalyses 5058 .!) Environmental Testing Solutions, Inc. P rojcd uu111bcr: H.cvicwcd It _1 : Control 11.3% 22.6% 45.2% 72.6% 100%
I
pecie~: Cerio./apnnuz d; bia Client: Senuo -ah '{ucJe,;_r Plant- Sv-rr*:Jted
' D!llA Clzem I'
I' 11.3% I I 22.6% I 45,2% I I 72.6% I 100% I I I 100% Intake I f Page 46 of 96
I Page-:-- of7 I Species: Ceriodaphnia dubia I Client: Segw;vah \iuclear PlanT- 'wn-treated Date: tO*Z&-oi Concen- Parameter tration I I J 11.3% I 22.6% 45.2% 72.6% 100% 100% Intake Page 47 of96 .J. ~c:reb. <.o~~"rr-< v,..,._ue.~ 1-c.,N.e..h, lt.1lO~I..'l IN ,.)tJ:.,T I{I*H~~ 5PA<£~.
,._"~ u.Je.';;. C.OWFtf.Mth
?a~e l of6 Chronic 'Ahole Efi1uent :*oxi*:ity T"'sr *EP_\--321 K '.>J :_~ \fcthod lOi)l, ~pccies: Pimeplwles prumei,,-,.
Client: TVA County: Hamilton Facility: Seguovah ~uclear Plant Treatment: lTV-treated NPDES #: TN 0026450 Outfall: 101 Project #: __._s .-.. . cL..S.u8L-___ Dilution preparation information: Comments: Dilution prep (%) !!3 22.6 45.2 72.6 100 Each concentration was treated Effluent volume (mL) 282.5 565 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2217.5 1935 1370 685 0 to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences. Test orJ?anism information: Test information: Organism age: 1-"" .S~ M nuf'lC.. 0\l:) Randomizing template: .f>L.O.. Date and times organisms /()*'Z.1*0cf lloOO Incubator number: were born between: 2& Organism source: "TCX. &Ale.\\ {'p IC)* ~,-of Artemia lot number: ~160lla.J Transfer bowl information: pH= Temperature = oc Total drying time: l.*t.\\o-)~ 1.81 '2'1.1-\ Date I Time in: 1\ *o'-\-o8 11.'2.0 Average transfer volume: Date I Time out: \\-0~*0& lb'20 1.f~ Oven tem~erature: lo()'( Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time used time 0 2 3 4 5 6 7 Control information: Acceptance criteria Summa__ry_ of_test endpoints:
% Mortality: 07.. $20% 7-dl!)l LCso >l (X}Gf Average weight per initial larvae: o.'i'-1.. '*: . {,];'ti~:;;:;;; NOEC 100'1 Average weight per surviving larvae: o.~'-"1. > 0.25 mg/larvae LOEC "l tco7.
ChV >(007. IC2s } I001o Page 48 of96
'. "';"(, ~*~* ....,,
Species: Pimephafes promdas Dare: Client: TVA I Sequoyah ~uclear Plant- lJV~treated Survival and Growth Data Day COl'i_TR()f-_ _11.3% }~.6% A B _(:_ _p_ E _!'_ G _H I_ _.f K L 0
/0 ID IO (0 IO 10 /0 ID IO 10 lO ID 1
ID IO /() /D /r:J IO to Jo IO IO 10 10 2 ID 10 /D /C) IC 'o {() IC 10 to /() 10 3
/Q /D /D 10 IO /rJ lb to ID IO 10 10 4 /0 /() /{) to I0 10 /{) lO lt) 10 ID 10 5
10 /D /0 /Q IO 10 10 /0 /Q to ID 10 6 JD /0 /D Jo It> 10 to to IO IO I(J 10 7 ,t. . /0"' I OS.tl\ /D /() 10 /Q 10 10 lb~ IO ID A= Pan weight (mg) Color identification:\ .\d.\l.C Pt~o 1'-{.ulf ~~-~~ 1~.33 13.1 s 14.~Y l&.tt Ill. \1. tt.f..S3 lll.11 ILI'l l~.lo"l Analyst: ~ B- Pan+ Larvae weight (mg) Analyst: \jL~
\Ct. '2-'\ *o. ! 0* 'OO IS.l~ \4.)5 l't% -z: -
V.i~~*' \4.t.\'--\ Z...;.J<j \.0('5~ 1<1. lio tt,.z3 z,o:lll Larvae weight (mg) =A- B l\.rh 1-\.\\o l\.'-\0 S.t"l '--1~ i \q (pJl_ \g.. 7..L. 5.03 L-1. Vl ' 0'1 L,.O(p
>. \0* . ~-
Weight per initial number n'-l.tF\ 1"'. ~'1.- C>.\.:1~*2- o.~l'ift oJoDL\ o.IPD~ D*u.'*"o oS 0l. 0 ~1it of larvae (mg) ;V*.. \.\\\o 0. io \'-\ \j.\.9 c:;o') V*
= C I Initial number of larvae Average Percent weight per reduction initial from control 0 .t.\lo '- O.l.>\2. -?i1.~ '* o.SS\ - \~.1.1.
number of (%) larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: &-- Comments: Page 49 of96
.'opt'~H::~: Pimephal,<; ;7!itn2/u'>
Clien :: T\--1. Sequoy:1h ~ uck1r .?!ant- C\ -:rea ted Survival anrl Growth Data Day 45.2% 72.6% 100'% M N 0 p Q I R s T u I v I w X 0 10 IC ID /D 10 /I';) /0 /Q ID /() JO /Q 1
/D f() /D 10 It> /() 1'0 I(J IO 10 JQ ID 2
j\) /0 /0 /{) 10 {b /0 ID 10 /0 ID 10 3 IO 10 10 10 lD /0 /D 10 to to 10 to 4
/Q /0 to fD /0 It> /0 10 lD 10 10 /(J 5 /f) 10 IC ID J(J 10 /D 10 /(J 10 10 10 6
IO /C /{) ID /Q ID I(J It; /(j /{) ID 1\) 7 ID /0 /f) ID to /0 ID /D IO 10 tO 10 A= Pan weight (mg) Color identification: l, btu.e 13.88 l~.t8 13.81 1l{.\H I'{ .1l\ \\{_(.,1 lS.33 1~.~~ 12..Cf2. li,S(p 13.Z.S llt'10 Analyst: UCH?, B =Pan + Larvae weight (mg) Analyst: ~l~ jCf.\o) \0.(;-b IG.3'-I L.\."l.lJ iq.Tz. zo.% 2. \.<1 (., Z.o}z. 18.'1B 7c.q3 IB.loi.? zo.l\~ Larvae weight (mg) =A- B s:n s ..;$) s. . n G-3~ ')S~ 5.11 ~.t.o3 ~,'b5 55~ s5l 5;4\ (,_o') Weight per initial number of larvae (mg)
=C I Initial number of larvae
().~'"i') v~t.\'v !rv.<;4"'1 O.l,;~cl oS'S'O . , we~ o:"Slc\ O.lol93 u. vu eSSie c55l t7:5q \ 0 .~os Average Percent weight per reduction initial from control o.S,'5 -1.'-\.lo io o.(."Z.L. -3~.11o o.seo~ -n.1.7.. number of (%) larvae ( mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Calculations and data reviewed: L Comments: Page 50 of96
Page ~ or~ Species: Pimeph<Ifes p~umelas D:irt: _ _(JL_ z~ . 04__ --*--- Client: TVA. Sequoyah :"iudear Plant- CV-trt:.tted Survival and Growth Data Day 100% Intake i y i z I AA BB 0
/0 Jo IO IO 1 /D 10 10 10 2
ICJ I() JO /0 3 10 10 IO IO 4 IO 10 /6 10 5
/(J /D /Q /0 6
lb /0 /(j /C) 7 10 /0 to /0 A= Pan weight (mg) Color I. l:llu.e identification:tt'T"l. 13.SLI 13.ttS \S. $~ ~ Analyst: LA1?J l't-~* B =Pan+ Larvae weight (mg) iC/.35 Analyst: {bL~ Zo.~') 'Z-Lib G.l.v'-\ Larvae weight (mg) =A- B S.'6\- G.l:t' S97. \o. . 13 Weight per initial number of larvae (mg) o5'6\ :-.~vo IJ. o::s4 . ,_ i:),\o13
= C I Initial number of larvae Average Percent weight per reduction initial number of from control
(%) o.<..Zl -3$:1'7. larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Calculations and data reviewed:~ Comments: Page 51 of96
~ TVA I Sequoyah Nuclear Plant, OutfalllOl- UV-treated ~ ~ ~
October 28- November 04, 2008 U1 N
..,.}...,
0 I,C Pimephales prome/as Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses ".:, Environmental Testing Solutions, Inc. Projec( uumlH'r: sus~ Not for ConlPiiance Assessment, Internal Laboratory QC
--r-y-- ~
Cuocentnttion (%) Rcplicutt: lnitial number of FinMI number of A=- Pan weight B =Pau + Lan'ae Lan*.ll.e\'"eight (mg) Weight I Surviviag Mcu\\cigbt/ Coefficiut of \'llrilitiun Weight /Initial number Mc:.~nsun'i'>>l Mc.~n\\cight/ ~~~mril*nt ul *---~.cnt1cdudiun frum lan*ae larvae (mg) ~eight (mg) *A-B a umber oflarvac (mg} SurviYiag number of !hl~n wdcht ,.., ~~~rvhil*l of lan**e (mg) (%) lttitialuumbcr ul \afiatiuu 1.\J. .... "u;:l<~ ronlrul t%) lan*se(mg) 11\l,.b..rnrL.,-wu)(o/*) !Mr\'itC l1H~) )""I n!lll~* "~"\(,., ,.( I** *4l]
*~ .
A 10 10 14.40 19 29 4 89 0 489 0.489 B 10 10 14.64 18.80 4.16 0 416 0.462 8.8 0 416 100.0 0.462 K.K !'\ 111 applicilhlc Control c 10 10 14.34 I 8.74 4 40 0.440 0 440 5 02 0.502 0.502 D E 10 10 10 10 14 33 13 18 I 9.35 18 96 5 78 0.578 0 578
------- 1---
F 10 10 14 64 20.78 6 14 0.6)4 0 614 11.3% 0.612 3.8 100.0 O.o 12 J.~ *32.*1 G I0 10 13.12 19.44 6.32 0 632 0.632 o o22 H I I0 10 10 10 14 12 I 4.53 20 34 19.56 6.22 5.03 0 622 0 503 0.503 J 10 10 14.27 19 16 4.89 0 489 0 489 22.6% 0 551 11.5 I 00.0 0.55 I 11.' 19.2 t K 10 10 12.19 18 23 6.04 0.604 0 604 L 10 )0 14.64 20.70 6 06 0 606 0 606 M 10 10 I 3.88 19.63 5.75 0.575 0 575 N 10 10 14.28 19 68 5 40 0.540 0 540 45.2% 0.575 7.8 100.0 0.575 7.8 2~.0 0 10 10 13 87 19 34 5.47 0 547 0.547 p _Q 10 10 10 10 14.87 14.14 21.26 19.72 6 39 5.58 0.639 0.558 0.639 0.558
--------- --*-r -* !
R 10 10 14.67 20.46 5.79 0 579 0 579 72.6% 0.622 I 0.2 100.0 0.622 I 0.1 -34.7 s T 10 10 10 10 15.33 13.44 21 96 20.32 6.63 6.88 0.663 0.688 0 663 0.688 I u 10 10 12 92 18.48 5 56 0.556 0 556 y 10 10 14 86 20.43 5 57 0.557 0 557 100% 0.564 48 100.0 0.%4 .J H -l.?..t w 10 10 13 25 18 66 5.41 0 541 0 541 X 10 10 14.40 20 43 6 OJ 0 603 0.603 y 10 10 l3 54 19.35 5 81 0 581 0 581 100% Intake z 10 10 13.65 20 25 6.60 0.660 0 627 7.5 0 660 100.0 0.627 7.5 J;i.7 AA 10 10 15.84 21.76 5.92 0 592 0 592 BB 10 10 14 31 21.04 673 0 673 0.673 Outfall 101: MSD~ Minimum Significant Difference Dunnett's MSD value: 0.0791 PMSD- Percent Minimum Significant Diffcrcricc PMSD: 17.1 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the *control and treatment that can be declared statJstH.:ally Sl~nlflcant 111 d wlHdc cllh.t;n\ !,!', . !I)' test lntake: Dunnett's MSD value: 0.0601 Lower PMSD bound determined by USEPA (lOth percentile) ~ I 2%. PMSD: 13.0 Upper PMSD bound determmed by US EPA (90th percentile)- 30%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratot-y Vat iabdity Snaiy (US EPA, :*uo I J. ( J~L I' \ ~UO lll) US EPA 2001 a, 2001 b. Fmal Report: Interlaboratory VariabilJty Study of EPA ShorHerm Chronic and Acute Whole Eftluent Toxicity Test Methods, Volumes 1 and 2~Appendix EPA-821-B*O 1-004 and EPA-821-B-0 l-005 US Environmental Proleuton Agt'.llt..:\ ( 'HJ<.:JilllJit, OH
'"0 ~
(1Q TVA I Sequoyah Nuclear Plant, OutfalllOl - UV -treated
~
VI tH
October 28- November 04,2008 0
\0 0'1 Statistical Analyses ,) Environmental Testing Solutions, Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 10/28/2008 Test ID: PpFRCR Sample ID: TVA I Sequoyah Nuclear Plant, Outfall IU1 End Date: 1114/2008 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV -treated Cone-% 1 2 3 4
~ *~~~ 0.4160 0.4400 0,5020 ________ ,, --
D-Control U.'t~':IU 11.3 0.5780 0.6140 0.6320 0.6220 22.6 0.5030 0.4890 0.6040 0.6060 45.2 0.5750 0.5400 0.5470 0.6390 72.6 0.5580 0.5790 0.6630 0.6880 100 0.5560 0.5570 0.5410 0.6030
---~--------*-*
Transform: Untransformed !-Tailed ,b(Jlollic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD M.:an __ N-Me<111 , D-Control 0.4618 1.0000 0.4618 0.4160 0.5020 8.778 4 0.5M2 1.0000 11.3 0.6115 1.3243 0.6115 0.5780 0.6320 3.846 4 -4.560 2.410 0.0791 0.5642 1,00()() 22.6 0.5505 1.1922 0.5505 0.4890 0.6060 I 1.480 4 -2.703 2.410 0.0791 0.5642 1.00()() 45.2 0.5753 1.2458 0.5753 0.5400 0.6390 7.842 4 -3.456 2.410 0.0791 0.564! 1.0111)() 72.6 0.6220 1.3470 0.6220 0.5580 0.6880 10.160 4 -4.880 2.410 0.0791 0.5642 I OU0\1 100 0.5643 1.2220 0.5643 0.5410 0.6030 4.759 4 -3.121 2.410 0.0791 0.5642 I lliHJO Statistic Critical Skew --*- Kun --* 0.94403017 0.884 0.12865374 -I.:J(ll~IP*I 4 1?7R41 15.0862722 MSB MSE 0.01310714 0.00215657
>100 >100 >100 >100 >100 >100 >100 squ!IJ! /IJ .}1),(}8<illl<i**liV
~ ~
IJQ TVA I Sequoyah Nuclear Plant, Intake- UV-treated
!'1) !J1 ~
October 28 - November 04, 2008 c
~
Q\ Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 10/28/2008 Test ID: PpFRCR Sample ID: TV A I Sequoyah Nuclear Plant, Outfall I 0 I - Intake End Date: 11/4/2008 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated
-'--~:::..:..C.'-----::------:--------,,------------------------------------- ----*
Cone-% 1 2 3 4
~----~~------~~~--~~~---------------------------------------------------------------------------------
D-Control 0.4890 0.4160 0.4400 0.5020 100 0.5810 0.6600 0.5920 0.6730
--~ ---- -**- -----.
Transform: Untransformed !-Tailed Isotoni,; Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-f\k~" D-Contro1 0.4618 1.0000 0.4618 0.4160 0.5020 8.778 4 0.544 I I .0000 100 0.6265 1.3568 0.6265 0.5810 0.6730 7.455 4 -5.328 1.943 0.0601 O.Htl I OilOll Critical 0.749 1 1?7Q??Qil 47.4672279 MSB MSE 0.05428513 0.00191229 Linear Interpolation (200 Resamples) Point % SD 95%CL(Exp) Skew
!COS >100 lC10 >100 IC15 >100 IC20 >100 *JC25 >100 IC40 >100 IC50 >100 sqn/01 /II ..'8 IJNdut,, 11v
'"0 TVA I Sequoyah Nuclear Plant, OutfalllOl - UV-treated ~
C1CI tl) October 28- November 04,2008 Ul Ul ..e., Pimephales promelas Chronic Whole Effluent Toxicity Test
'~ EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses \ ,.r;;,i Environmental Testing Solutions, Inc.
Project numlocr: 5058 Control 11.3% 22.6% 45.2% 72.6% 100% 100% Intake
,,.~,r::.~!T'-"" :11 ***:on<J ~**~:*"!'! ,.,(
' Species: _?imcpiwies promela.l C:ient: TVA' Scquo_vJh ~udear P',lnt -LV rr~:tLd ' Chemisrrv: 11.3% 22.6% 45.2% 72.6% 100% 100% Intake
Page 6 of 6 I
~"'nrun'l1emai,.-<'mn9:~rution!.,ln<
Species: Pimephales promelas C'ient: TYA / Sequoyah ~uclear Plant- CV-treateJ Dare: /O* U*O~ ' Concen-tration Parameter CONTROL I I I 11.3% I 22.6% I 45.2% I 72.6% I 100% I 100% Intake Page 57 of96
P1ge _r_-+"-- I ")':lge _)___ of __ /_ I
.... ~--~-**---,---
I
' Environ~en~:d Te~<:>'g Sci~..:::of'ls. ~nc I otal Residual Chlorine (Orion Electrode Methodl Orion 97-70)
I Matrix: Water, RL = 0.10 mgJL Meter: Accumet Model AR25 pH/Ion Meter I ~ tJ I Analyst Date analyzed o-"-
._f-_ -_-_._-<-l....._.L:-=l='j(==-=C~*-<;f=:
I Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. I L ab ora ory_ con tro I st an dard Reference standard True value (TV) Measured value (MV) % RS=MV /TV X 100 I number
f:.A1 SS5 7"'1 (rng/L) 0.50 6 (mg/L)
'-1'8-q (acceptable range= 90 to 110%)
CjJ,lf'~ I Sample characteristics I I Sample measurements: Sample lD Sample characteristics number I
-.:~~
I I l l Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. I, L aboratory contra l stan dard: Reference standard True value (TV) Measured value (MV) % RS =MV /TVx 100 number (rng/L) (mg/L) (acceptable range= 90 to 110%) I 1-NSS5 7<1 0.50 0' Lj G 2_ qZ-,1% ( Page 58 of 96 Reviewed by ~[_...,~~----.,----1 Date reviewed [ fT-' tl* Cl!
ID I Page P1ge __ ; _ of_/__
-~ ~~~~ ;,41!
I
*rs:~ ~
I Total Residual Chlorine (Orion Electrode ::VIethod, Orion 97-70) I Yfatrix: Water, RL = 0 10 mg!L Meter: Accumet Model AR25 pH/Ion Y!eter I ~,yt 11/' I Analyst Date analyzed =~~5 6-C!'{ Iodide reagent: Acid reagent: JA//2_3 2!
./1/;2. J 0 C

,
I I L aboratory contro l stan dard: Reference standard True value (TV) Measured value (MV) % RS=MV /TV X 100 I number
-;J.,/IJS55 }1 (mg!L) 0.50 (mg!L) o .. <::s J (acceptable range= 90 to 110%)
lb~. z~ I Sample characteristics I I measurements: Sample ID Sample characteristics I I I I 1. Note. All samples were analyzed in excess ofEPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS - MV I TV x 100 number (mg!L) (mg!L) (acceptable range= 90 to 110%)
.Y/JJss*s7'f 0.50 0.'-1'6{ 9'1 / (, ~
I Page 59 of 96 Reviewed by 11-------'d:=~L---:----1 Date reviewed L--.!.:I0=-*~30~-o~t___J
I _ '-i \.D _4 '/. ?age P1ge _l_ of_/__
\\
I I ,..... En,Jironr.,~r.ra1Te5tir.~~ ::ic:.o~s. '"'** Total Residual Chlorine (Orion Electrode Method, Orion 97-70) I Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter I Analyst Date analyzed I LfH2,
\\- 0 l _ 08 I
Iodi~e reagent: INIZ.3Z.I Ac1d reagent: 'T.NR-3 0<o I Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. I L aboratory contra I stan dar d: Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 I ~sSSl'l number (mg!L) 0.50 0.?\~ (mg!L) (acceptable range= 90 to 110%)
\01-S ;_...
I Sample characteristics I I Sample ID Sample characteristics orine I I Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. L ab oratory con tro l stan dard : Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 number (mg/L) (mg!L) (acceptable range= 90 to 110%)
-a:N<:,SS1 t:t 0.50 ().4cn qq, 4'/.
Reviewed by 1-f---:<jf6>>--=-----J Page 60 of96 Date reviewed L[-~i\._i='O_.....,tL-or.3.L __J
Page Page _ _ _ ,Jf_.,----- Alkalinity I (S:.\1 2320 B) Matrix: Water, RL = l.O mg CaC03/L Analvst I _:_i~~._cr_*-':;;_;- - - - - 1 Date analy;ed ==1=,_~=*=:===\i=***===== 1 Tmime.ceoimmptliaetteedd 1--1 Titrate samples to TI [ H*-'>;;>::> pH= 4.50 S.U. I Titrant normality_ and multiplier determination: pH of Deionized Titrant Normality check Begin End Total Normality (N) of H 2S0 4
= (5 ml Na 2 C0 3 x 0.05)/E pH Factor or Multiplier = (N x 50000)/ 100 ml sample water reference standard ml ml ml = 0.25/E =Nx 500 I = 4.5 s.u. number number J* '~1- ~. ,;:, *rT~f ;.~:_;*;~ . . . *r r....!-s:; ~it 1 c l.' j.J,. I (E) !.)..I (acceptable range= 0.0180- 0.0220)
(: .(> c~ ~; ; j c: ::;, I L ab oratory contro l stan dard: Reference standard True value Sample Alkalinity (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range I J:N~.,5 ((en-(mg CaCOJiL) 100 {ml) 100 ml 1..1.1 ml
.Jt.v C( \)
ml IC ::) (\ r::.r l ...
= 90 to 110%)
CI?)O{.c I Multiplier Alkalin;ty (mg CaCOYL)
%RPD=
{(S- D) /[(S+D)/2)} X 100 (acceptable range=+/- 10%) I ~~~~---r-------------4--~L-4--==-~~~~~--~-------r----------~ IC3 D s I atrtxspt'k e recovery: Reference standard Spike value Sample Spike alkalinity (A) (mg CaC0 3/L) I number (SV) volume Begin End Total Multiplier (mg CaC03/L) (ml) ml ml ml
r ~.r:)*~ tt c. .t K,' ,..,
.~v lCO 2-7. 1 t '") .~ ")>:,.\, j()(c iC.3 liC I Sample alkalinity (B)
(mg CaCOYL) Measured spike value (MV) MV =A -B
% R= MV /.SV x 100 (acceptable range (mg CaCOYL) = 75 to 125%)
I Samp*e I measurements: I.RC' 5o " l ['(' ( '((' Sample volume Begin End Total Alkalinity I Sample number 1 o. iS*o8 m" ':J Sample ID 1-ho (ml) 1c*t* ml
-'~- v ml >-rJ, ~
ml
..) .Q h
Multiplier
!C ~
(mg CaCOYL) t "(\ 1D~ i ~>- L.Je) l .:>.'
- /" s (!. tee I
(/(1 10*l'l*u8 SS~,_.v i-bO S',\ '6 :j ~ I 2,2 I 0- 13 .ut) (+ rnw::. H 1..tl :i ;j l~f s t).tl l£* I I 10* 1'5 ,(..-6 63tf'
'SSh) /+/-.*~tl: JOOCJJ'-\
HvD :.~_l(:~ lS j ~- ...,-
-~~ ,g ll \ .3*'+ .;~Cj ' '-':J j6 )
I 2r.: 1
/*y,*,)e'-'L.J I "-... ~~); ~3CXJ~ J I JtJOe-!)5 t u<.A:}7¥>
Jtn
~:i ~1 ) ~
c;;, ') ~. 2k ~
'I ;(C .I .;;< .') l \ ~(:
c~iu; I Page 61 cf%63J()j s \1) .ctb~d.J PLJ.C K
J
*~*.*j <; ~y, ~t' 3*r ~,* #" *. i't\. ~~*<J. IZ..CO Reviewed by: Date reviewed: f(). (q-(Jtf
I Page _ __ Page _ _ _ of ~ I Alkalinity I (SM 2320 B)
!vlatrix: \Vater, RL = 1 0 mg CaC0 3. L I
Analvst l Tim' initiotod i ~ Date analy;edl =:*=~;=**=,=-* =====
=-,
Titrate samples to Time completed ........__ -------------------~
pH= 4.50 S.U.
I Titrant normality and multiplier determination: pH of Deionize.Q. Titrant Normality check Be£ in End Total Normality (N) of H 2 S0 4
= (5 ml Na 2 C03 x 0.05)/E pH Factor or Multiplier = (Nx 50000)/ 100 ml sample water reference standard ml ml ml fl 1 '\IF. = Nx 500 I = 4.5 s.u. number number (E) (acceptable range- 0.0180. 0.~ *L I L a b oratory contro I standard:
Reference standard True value Sample Alkalinity (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC0 3/L) (acceptable range (mg CaCOJIL) (ml) ml ml ml = 90 to 110%)
.I't-..;::,~:. \.( L'1- 100 100 3-3S >-~]..t'j ~ 1-f* j\:,; el--l cr7 e*-Lt:-
Alkalinity %RPD = Begin End Tota*l Multiplier (mg CaCOJIL) {(S- D) /[(S+D)/21} x 100 ml ml ml (acceptable range=+/- 10%)
~~~~--+---------~~4-~~-+~~-+~~+-~~~------+---------~~~~
Duplicate (B) M atriX spt'k e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaCOJiL) (ml) . ml ml ml
~::r~,\J s~ tJ;.~>'1 lccc 5 &<~ f- ~),-l-- i i) _[' '1? [t)JO 2- ; ~5 (' c Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOYL) = 75 to 125%) '5i$*C r~J-\D c.*I--F.-;4:
Samp,e I measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID l_ml) ml ml ml Multiplier (mg CaCOyL) (' -1 ) p.. qJr-
') ,_, ' DA ,j iJ ">lfll K J_ !5t;J-';7 'fJ .l;~~~- !.:'I .~ - "..
t*-l Ji1~*)
lD3 ~r -bC'C>
(){I OIL-\. G \ ~LH.>I~ud I jC() {., .j q~ 5 '1 l -3S 1'1 R 101fc:,.o-z.. I l I cl 9, jC{ /. ().\/ I r
'*I ') .r;, ~8JC\<'i*O\c ,!, 3 J_ jCi 3 2L p ,*.{.!.].
1 'lt.t nt /1'"114. 01- ChctHx~n<.Dc- .c*. j .j
. ~~("'# ~:!, t: -~) -*. --- 0 \ 0-t-~ I .:=)CJ l OtJ ()I b. (:)"2- I z l ..J__
c-. c IJ l iI I (1 f l I *'-t\,ct_*, OlJol&.<rl .It -::r
-' il j ! 2: I ~ \j (. --.t.tf"';_..:' IJi l L~ 1(
ol*o*'-'* 03 fiokbuno N\1 I !O<> I. :2 : l ,.:):) 't t.-j- ~ \ ;4-Lj. Page 62 .r~J;Jo liD. 0~
.-l,. z. L- ~ ~--5;~;. ~~*t
~c.l i-} 'l .J_.
.q.<,
Reviewed by: Date reviewed: [ /0. 10* v0
I
*..)....,
Page ---'---
~ ?;1g:: ----'--of--"---
I \
~- ---~-
,"\v!rG.--;me,r

~a: -est1ng
-- ~~- .::.~!:..t.ons: .~<
A.lkalinity I :Vfatrix: Water, RL (SM 2320 B)
= 1.0 mg CaCO,iL Analyst 1 ;; ( I . . ___ --;...
I Date analyzed I===~:J~=i=~=;;=~====== Titrate samples to Time initiated Ttrne completed L __ _ _ _ _ _- ' pH = 4.50 S. U. I Titrant normality and multiplier determination: pH of - Normality Normality (N) of H 2S0 4
= (5 ml Na 2 C0 3 x 0.05)/E pH Factor or Multiplier = (N x 50000)/ I 00 ml sample Deionized Titrant c "" ~ End Total ~ -(acceprau*< ,,.,
water reference standard ml lii1- = 0.25/E =Nx 500 I 4.5 s.u. number number ~ 0.0180- 0.0220)
-~
I Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range
= 90 to 110%)
I .;: N <Js \!.J '5 t (mg CaC03/L) 100 (ml) 100
~;~l ml ml ")q I; ml cj.\ lt.3 Ci'~ q;:s,:;-lc I End Total Multiplier Alkalinity (mg CaCOJIL) %RPD=
{(S- D) /((S+D)/21} x 100 (acceptable range=+/- 10%)
~~==~--~-------------+~~L-4-~~~~~~~--~------~----------+
I I Matrix spike recovery: Spike alkalinity (A) Reference standard Spike value Sample (mg CaC03/L) I number (SV) volume Begin End Total Multiplier (mg CaC0 3/L) (ml) ml ml ml
~:t: "-\SS \.~"in-- 50 )00 ,5 ~'+ J'S l.* I o~*z.. \v,3 ~'-Jou I Sample alkalinity (B)
(mg CaCOJIL) Measured spike value (MV) MV=A-B
% R = MV I SV x 100 (acceptable range (mg CaC03/L) = 75 to 125%)
I samp*e measurements:
~\.J' ~\. lit-l 4,~\ 66').
Sample volume Begin End Total Alkalinity I Sample number o& 1O\'-\. c'-\ Sample ID h, .\e-hhvw 1\i'J? \ (ml)
/OC IS~V ml ml J..1S \i."
ml
'*.-:I Multiplier }'\).;
(mg CaCOJIL)
'll )
l ;{\{, + E)0 (,,_-I I 04 I 0 1\o. ()\.\ 0 g 101 &. (>L\ J i 3
<t-J -~:<
J~ ~t-
\ ~2,;1,, '? '+~ .l-l .t-¥1 <**-~. -----~----~**~~-"--~__..._- -~-- ..,___ --~-*- ~
I
~** ~-~~ ~ ~- -~ --~-c* ~ ~ ,/}-f, .:t~ -~-*"!
I
-~ ,. .,. -- ~--
_......- ~ I
.:~-;_::::.---~---'"" ~- ..
Page 63 o o.e:: Reviewed by: Date reviewed: IIU..jqll0
"~
o'- !:: Pag~ _ _ _of ~
\ --*-*--*-----~ ... -* Env1ronmer,ta1 -e.mnq Solutions, ~tK Alkalinity (SM 2320 B) .'vfatrix: Water, RL = LO mg CaC03/L p sc Analyst f-----""'-='-----i Time initiated 11----"C:::...'_.!'!-=2.'-"-.~'-------i Date analyzed 11- 1:: *2- _\) S Titrate samples to Time completed L. ---Lllf...._'_~::_CjJ.___ ____,
pH= 4.50 S.U. Titrant normality and multiplier determination: pH of Normality Normality (N) of H 2 S0 4 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na1 C03 x 0.05)/E = (N x 50000)/100 ml sample water reference standard ml ml ml = 0.25/E =Nx 500
= 4.5 s.u. number number (E) (acceptable range= 0.0180 - 0.0220) ~:2- ..r~~~*i J:'"IVSS:)i.e'i ":) 0 II. '1- )j.'f ('.O~Icl tc.C\
L ab oratory control stan dar d: b .o ;;l. () '1-- lo 3 Reference standard True value Sample Alkalinity (MY) % RS=MV/TVx 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCO}IL) (ml) ml ml ml = 90 to 110%) vo'+- 100 100 ~3.5 ,~S qev 9Le~
.J..NSs '1-3 10.3 Sample Alkalinity %RPD =
Sample ID volume End Total Multiplier (mg CaCOJiL) {(S- D) /((S+D)/21} x 100 (acceptable range=+/- 10%) M,at. Tl.X spt'k e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mgCaCOy'L) (m~: CaCOJiL) (ml) ml ml ml TNSS~o-:J- $0 IDO 6:~ lio-1 I D.;> jl)._3 Ito Sample alkalinity (B) Measured spike value (MY) % R =MY I SV x 100 (mg CaCO}IL) MV=A-B (acceptable range (mg CaCO}IL) = 75 to 125%) 59 51 ID'L e-zc samplel measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC03/L) 10 * ;z.t).o80 fhl~ \1z.D fDO ~~-\ c;!~ .c ,t::; 'l lv. 3 (pi I D- 25.LJ0A I I JJ.\) 1 dT~ h'lS ::>. laO I0 . ~5-D8b 4 ~~-~ 33.1 se. I ltl 5?:/~1-l ITS.._ /Do\342 ~1 351, I.Cj .Jo 633"1'3 \ If 1001343 ~c:: .IL1 6/.~ J.Ci 2-o 633-:N j 41- 100844 J-tD. I .;?t--
~1.$' d.\..0 /0,\&*C~ rn 11s vv I 4-f,_, Li<o.O s.q V\
to* 'ZS*of A I z._ c::-0 5. 5.7- #:)q lr AJ~ Jp-1.S-()l f> j 3 .. 5.1- tiS 5.~ - ltD Reviewed by: Date reviewed: j I\ .IJ 5./T&
r:-* I Page
?age -------"'-'----0 f _ __ )..,
I
'\- **- -~--* . - - - *---------
rn.moniT'ental-estrnq .:i:.1ut*ons. ;nc Alkalinity I (SM 2320 B)
.\.latrix: Water, RL = 1.0 mg CaC0 3/L ~-)* * .
Analyst
~**_* - _-~_-. .;*-_..,_ -=-----t I Date analyzed r--'-,~'""'.~""'~--'--"---.1-~-.'$'_ __, Titrate samples to pH= 4.50 S.U.
T! Tmimeceolm:mp*tJ*aetteedd 1 . ----.. --- ..~.*. ~~; Titrant norma/it]'_ and multiplier determination: I pHof-Deionized Titrant
~mality chec .... D. =
in End Total Normality (N) ofH 2 S0 4
= (5 ml Na 2 C0 3 x 0.05)1E pH Factor or Multiplier = (N x 50000)1 100 ml sample ~.so- 0.0220) water reference standard ml ml mr =Nx 500 I = 4.5 s.u. number number (E) - r--~----r'--'
I L a borat o_ry con tro I stan d ard : Reference standard number True value (TV) Sample volume Begin End Total Multiplier Alkalinity (MV) (rng CaCOJiL)
% RS = MV I TV x 100 (acceptable range (mg CaCOJIL) = 90 to 110%)
I (ml) ml ml ml
..Jl'-.!'_;s l{C1- 100 100 i\::2.~-k 1\<) ~~.~ Cj_Cj 1\.\3 ll:2.
I Sample volume End Total Multiplier Alkalinity (rng CaCOJIL)
%RPD=
{(S- D) /((S+D)/2]} X 100 (acceptable range=+/- 10%) I
~~~~-+--------1-~~-+~~-+~~+-~~+-----1-~-----~ .\.,- Duplicate (B) ,;n, I M,atnx sp1'ke recovery:
Reference standard Spike value Spike alkalinity (A) Sample (rng CaC01/L) I number (SV) volume Begin End Total Multiplier (rng CaCOJiL) Jml) ml ml ml 2C ~~ :::.s t* ~o -:r ~D \CD r.:n.1 31.( lc '+ 1<>.3 llO I Sample alkalinity (B) (mg CaCOJiL) Measured spike value (MV) MV= A -B
% R = MV I SV x 100 (acceptable range (mg CaCOJIL) = 75 to 125%)
I Samp*e I measurements: 5~ 5-2 ICJ..I..C:l:c Sample volume Begin End Total Alkalinity I Sample number 0&10"'2.1*0\ TVA~NiLh Sample ID
~I 5D (ml) ml ml 37'5' t-ft ~r 3.2.
rnl Multiplier
'2l )0 .3 (mg CaC01/L) l._;.(t z.. -~ z I 0&\02'\*0\
o a' o~\. C> \ 3 L£,7- *-P q t)-60 '-+! 1, ;s:;,
t_\
tz) L,t.,~ lt* \ lo& so'l-1*01- 11A9;.,J I f.JI '3 7_
' L*l *
()_(\ 3 '2- -*.) I oct JC"Z.'\.OL OK 10 '01* 0'2-J J L 3 3L J:_J;1-. v..?> qq ~
~I \ ,,
lz) L:*'f
*-r if*
1 ) I _n.E-Io"'2..1.ot nf'ID"2.'\*01 lvA.-SL.t'J IDl UJ \ I z_
'iC. .I 12 .c.' ~~ '(:,
ifc.L
~-~ I 3_.-.
II 1:1 (;<) l lr-+ lc ~.( I Page 65 rfO&"I0-31*0\ I 3 llr-1 2i:,.\ )C, 1..)-- i ~[ Reviewed by: Date reviewed: [ j(-0.3 *LV
~-- Page _~-~ I ?1ge _ _ _ of ____ I A'. ---- --~---~------- EnVIfQ!"Im~fltaiT~sfir;g Solutions. 'nc Alkalinity I lSM 2320 B) Matrix: Water, RL = 1.0 mg CaC0 3/L I Titrate samples to pH= 4.50 S.U. I 1 ura"' "u pH of Deionized Titrant rltinlier determination: Normality check Begin End
-~--
Total
....__Normality (N) of H 2S0 4 ;(SmTNa~~-05)/E pH Factor or Multiplier = (N x 50000)1 100 ml sample water reference standard ml ml ml = 0.25/E **------ -- =Nx.SOO I =4.5 s.u. number number (E) (acceptable range= 0.0180- 0.0220) ------t**
I L ab orato_ry_ contro l stan dard : Reference standard number True value (TV) Sample volume Begin End Total Multipli~r Alkalinity (MY) (mg CaC0 3/L)
% RS = MY I TV x 100 (acceptable range (mg CaC03/L) (ml) ml ml ml = 90 to 110%)
I _l r--./.::. ':::> i.e C}- 100 100
-1 \i l c:)Cl e:; Cp+ I;:; .,; Cj-*j CIT;.!(,_
I Sample volume Begin End Total Multiplier Alkalir.ity (mg CaCOJiL)
%RPD =
{(S- D) /[(S+D)/21} x 100 ml ml ml ml (acceptable range=+/- 10%)
~~~~~~~------------~~~~+-~~~~~r-~~+-------~-s~--------~
I D I M atnx spt e recovery:
~
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume ~'&!I Multiplier (mg CaCOJIL) egm ...... Total I (mg CaCOJIL) (ml) ml ml r----.@1 I Sample alkalinity (B) (mg CaC03/L) Measured spike value (MY) MV=A- B
~'"--......% R= MY I SV x 100 " (1K$able =7 l~S%)
range (mg CaC03/L) I samp. e measurements: Sample volume Begin End Total Alkalinity I Sample number 0~1()"'2..!- 0'- -rJ~ts,b tJOv ;Nit Sample ID (ml) 50 ~C{,") ml ml
~.J.' l ml ? --I
), :7 '
Multiplier jv.2 (mg CaCOJIL) l.: l..e 0&101.~. 02 2 -:u_, 0 ; "1 t ;\ lr '\ I oRIOo\*01- j J 3 ..J I ~:.l.'l
2_( ~- 0 39 cl jc ('1 I)
J_ I s*c I c-- t..J./ __ ., ll.---
? --------
I / I Page 66 f Q(\_
-~ - -------- '---*~~
I LD3. t,t) J Reviewed by: J Date reviewed:
I Page Page of_,.__*__ I Total Hardness (S"Yl 2340 C) RL = ~.0 cr,g CaCO, '- Analyst 1 ;l;') L r--'--'-"--=-------i Date analyzed i( 1q C.::; I Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA I reference number standard number ml ml m1 (E)
= 0.2/E (acceptable range= 0.0180 - 0.0220) pH Factor or Multiplier = (N x 50000)/ 50 ml sample =Nx 1000
_t:Ni2..3c~ .:I-NS:; 5\..-v (1 1 CJ1 e .C;>. t> Lc D.O ,{u .~ L ab oratory contro stan dard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaC03/L) (ml) ml ml ml = 90 to 110%)
]::l'JSS 511 40 50 l"l.L.' c{l ':) I <=j ~c.2.. 3-:?0 qk:t'?c Sample Hardness %RPD=
volume End Total Multiplier (mg CaCOJiL) . {(S- D) /[(S+D)/2]) x 100 s Cj") zo.2-Duplicate (B) D
-1 i.. .;(t.;, .::L 3C <j s.rl ...1... C\5 jccC"lc Matrix spike recovery:
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (mg CaCOJIL) (ml) ml ml ml
..TN~S 5'11 .t-fO 5o ~v).. 3;1./ ~,., 5 2.C .*2-- 1 ::;o Sample hardness (B) Measured spike value (MV) *o;. R=MV I SV x 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaCO}IL) = 75 to 125%)
clS' 35 "l '6..,;:7.c Sample I measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mf! CaCO}IL) TV=ND Blank (should be= 0 mg CaC03/L) 50 .3,~.1 ?;:<} 0. ;::;, z_;;,,-z_ NO 10-1'5.'-15 m~-t s. Hz_D .3ll 31.3 4~ q~ j 10--16-a? ?.,1 .:>.. L.!.j ")' '"f.?... ?{5"
,10.1~1...-iB S~'--u H:z.fl *'-fl C) -'I~.;- ~.C .t...fD lO '\'~-~-*() 11 (II \ls i-1 z.0 .<.!.?,,I) 4~.C ti-S" <-11 10-IS..UO ~s. i./0 4\-z_l) [1.0 ~..< .::l. ..;) ~ ~p,~;-'-1~
o&'IOI~* 0) FO~wcJ I ,)_")., '-t I I . 'I -sst c& IO\Co .oz_ l 2 4i 15"'2.. '-+. i £':3 n4' I 0 ld
0 lo J 3 ,_.__
'62.. i:) i ~t '1 +-ef': P,<;.< l'i Page 67 _eArnt\.1 . C2- Chattanuoc,a t 05 :2, I l l.r:; 44 I/ 1) ---- 1'>\C Note: If >15m I of titrant is used, sample must be diluted. Reviewed by: }tj&\..... Date reviewed j l ()
lq t..~
I P3.ge Page J- of ) .. I Total Hardness {S.:YI 1340 0 I Analyst t--/ __,_t~;.,_,( = - * - - - - - ! R..L = J.') mg CaCO,iL Time initiated 11----=-=-----=-----.:::::::..<lll~!~.--1 Date analyzed "-1-"-"!t,_*._,_1 -'.:;,_._*;~<:.,_,_ ___, Time completed I
. _ __ _ _ _ _ __J Titrant normality and multiplier determination:
D. Titram Total Normality (N) of EDT A pH Factor or Multiplier I l*v " J<', " reference standard ml ml ml --~ = (Nx 50000)/ SO ml sample number number (E) (acceptable range= 0.0180- o:O!!G~. --. _3.Nx 1000 I L aboratory contro l stan dard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 I number (TV) (mg CaCOJIL) volume (ml) Begin ml End ml Total ml Multiplier (mg CaCOJ/L) (acceptable range
= 90 to 110%)
r..~ss s1 \ 40 50 nr:s JY v ..,.?.l ;(c~
. _j.- ~2 IGS\-l.o Hardness %RPD=
End Total Multiplier (mg CaC0 3/L) {(S *D) /((S+D)/2!) x 100 atrrx sp1 e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (ihg, CaCOJIL) (mg CaCOJIL) (ml) ml ml ml J::NSS 511 eo 2~ ~~ J.f di.O l.,;;\( (2.) 2_o .*?.- ,;( '1 0 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaC01/L) MV=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) 1qo <()a l eo~D S amp,I e measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC01/L) TV-ND Blank .... v ulg c..aLUJ/L) 0& 10 \t. 01 (}-uJ:tr~ "1 )r,r;>, 3 tt:; ~\.0 .305 55 12) 2.0.2 ;t,~C o&HH'-l. 03 ~~\I r~ t:,.,DJ~A_~ 00 I I Ko ~*" *) 3S.<, '-~ I Y.;t 0410it.,c:T'll I 7. I :3$ .\c -'+t .It- ;rc .Lfc otJo'Q .ol-j 3 I "'-iC'.~ 4;: -, dl Lj,;J.. 0£. IOI'J. ()~ bJ\d ~cJI-0002.- J .'k*1) 2,.~.1 d. I *+.J.. OS. IOit...u'-\ \ 2 3;;l, I ~t' I .Cl 3(.(' b& 1cll2. o~ j 3
~ ~ (' '35 11 l. ct .-- ~v .JAJ 1-.7{>
Page 6Sl nf Qt;;
- e.-- I Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: J J.{_~~..t*L- Date reviewed / /6- jq.t.,()
I Page )'1 Page _ _ _ of ____(__--__ I -
---~----- -- ~p*,monr:1ent..li ~eH1ng 5o1Utl01'1 ,, !1C.
Total Hardness (S:VI .2340 C) I Analvst i hD \._ RL =: 0 mg CaCO,,L Time initiated Date analy;ed r- --i---l""_)--=---'-V-_-----l Time completed _ ll-L ~-\...-\...' I Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier I reference number standard number ml ml ml (E)
= 0.2/E (acceptable range= 0.0180- 0.0220) = (Nx 50000)1 50 ml sample =Nx 1000 J!J-51~
IJJ~svlev OD Q .\J C}.0 D-02C<3 w.e I a oratory controI stan dard: Reference standard True value Sample Hardness (MY) % RS = MV I TV x 100 I number (TV) (mg CaCO:VL) volume (ml) Begin ml End ml Total ml Multiplier (mg CaCOJJ'L) (acceptable range
= 90 to 110%) \ J.}SS *51-1 40 50 q_c,., I 1.'-1' 1.0 :lo.e Li2 10..J .o i~
I Sample Hardness %RPD= volume End Total Multiplier (rng CaCOJJ'L) {(S- D) I[(S+D)/2]} x 100 I I atnx spl'k e recovery: I Reference standard number Spike value (SV) (mg CaCO:VL) Sample volume (rnl) Begin ml End ml Total ml Multiplier Spike hardness (A) (mg CaCOJJ'L) 1~611 IJO so l(t).5 ~3.0 05 10.6 1-+0 I Sample hardness (B) Measured spike value (MV) %R = MV I SV x 100 (mg CaC03 /L) MV=A- B (acceptable range I ON (mg CaCOJJ'L) Lfw
= 75 to 125%)
115.-o'l Sampje I measurements: I Sample number Sample ID Sample volume (ml) Begin ml End ml Total ml Multiplier Hardness (mg CaC0 3/L) TV=ND Blank 9D 6 rJ) I I D 2<1 -btJf) (should be= 0 mg CaC0 3/L) m tt-s 1-1:2 o 5b 0,0 fJ cl M.o a'-Uo <--l.u li.O l qlP to .fJl;.ue a i ~1AL? 62 4.v f q(o I {LJJ;5,,L(J@ J 32.1 8lc.5' LL3 m o_£ 10- 0~ mHs v.J I 3<t;.~ ~Q;(.p \_1 l .. I 10- "2'5-c8 A
'()* ""l'S- o& &
2 3 i.\o.<o ~S.z 0.0 L\.~ Y.G 4A Cil.~* qz 4 PI-t I \b'(.'\*0~ f\
'n&1o21. o \ f\JA-~JJ 10 I I Z~>S-L--\.Lt ~La fl) lp '-\-.2.-
ln.f.J 2.2- {2.) iiZ I Page 69 .r£Mn01.'\. o\ l i 10.6 1'2--:6 z_Q i
~ '-*
B-6 Note: If >!Sml oftitrant is used, sample must be diluted. Reviewed by: Date reviewed I 1/ u0
I P:ige _ _ _ of_;_,_ I Total Hardness (S~I 23-10 C) I A:1alyst i Rl = l.O mg CaC:~':I L Time initiated !,--,- Date analyzed 1----+-+='------1 Time completed f-------=-,;-.:~---~...---1 I '------'--'----=---' Titrant normality and multiplier determination: Titi=am- ~malit}c£.h~~ k Begin End Total Normality (N) of EDT A pH Factor or Multiplier I reference number standard number
--**-mr- uti ~I (E) = 0.2/E (acc~ptable range---;-o:-OT81r=-u:-onor--- = (Nx 50000)/ SO ml sample -------.. 4-Wx 1000 I L aboratory contra I stan dar d:
Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 I number (TV) (mg CaCOJJL) volume (ml) Begin ml End ml Total ml Multiplier
;Za.e (rng CaC03 /L) 4-z_
(acceptable range
= 90 to 110%) )J\jr~ 611 40 50 JJeJ t4.J ')_Q IC6.b/.
I recision: Sample Hardness %RPD= Sample Sample ID volume Begin Total Multiplier (mg CaCOJIL) {(S- D) /((S+D)/2]} 100 I End X number ml) ml ml ml r.e t) zo,e; s H0 J(;,(tJ D
/_q -i I /G_1,(.p It]') J atrLX sp1 e recovery:
I Reference standard number Spike value (SV) (mg CaCOJIL) Sample volume (ml) Begin ml End ml Total ml Multiplier Spike hardness (A) (mg CaCOJIL) i1 r~ ~.Srt::;-:}1 0D 9~ VJ5 .:J. :;, 1 ;vo I J._) Sample hardness (B) fr..d-' Measured spike value (MV) t2) JfJ.A
% R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) ')c1 6i 1D 1.2 I S amp I e measurements:
Sample volume Begin End Total Hardness Sample number Sample ID - (ml) ml ml ml Multiolier (mg CaC0 3/L) Blank ~ TV=NB-(should be 0 mg CaC03/L) cg *c-rt. 01- -IJA ~!V i>Jr I 61) l*?n 5' dtf. t./ 3 .c1 ~0 8 fjl Cf IQ2q .oz... I 2 I ~y~-1 ,~R.'-J YO 3_5 I U* j o~ too\. o-z... '3
~- J Jt).'-f ..3"'7 I.<..- 3tl '}~
oi ac2'1-o\ *{JA.$&.'tJ aDl UV i a.; 3)-2. 3Y.-J 1-1 j;i) S'f-I o" lc?\.c \ J 2. J I 3Ll~ O!tO.Z I.Cj \ es
/.}-* ~~t 0~ 10~\. () \ l 3 ~.z_ :kU 20 J 1JA .Q3tJ J0.z.. Lll-1 3q g~
I M I 0 '2\ . 0 1.- l'l& 10"2 (\ .o 1-INiw ( I J_
~'SL)
I 'i2.1 1'-fL.l"i 3q
.;I \.)
I Page 70 ~tOil~~\* () 1-J 3 .~ '-1 3.<1 U.'\ {_., 3,1 - L~ Note: If >15m! of titrant is used, sample must be diluted. Reviewed by: I Date reviewed I
Sequoyah Nuclear Plant Biomonitoring October 28- November 4, 2008 AppendixD Reference Toxicant Test and Control Chart Page 71 of96
Pimephales promelas Chronic Reference Toxicant Control Chart
\ Organism Source: Aquatox, Inc. ~-~* Environmental Testing Solution;: Inc.
1.2 .- -.------,---,---,----~---,----.--.---~---.,-----~-,---- USEPA Control Limits (+/- 2 Standard Deviations) 1.0 0.8 0.6 0.4 1.2
-u ~
1.0 USEPA Warning and Control Limits (75th and 90th Percentile CVs)
~
e J) II'J N 0.8 u
~
0.6 eo: 0.4 - * - - - * - * * - * - * - - * --
"'0 I
t-- 1.2 Laboratory Warning and Control Limits (lOth and 25th Percentile CVs) 1.0 0.8 0.6 0.4 Test date 7-day IC 25 = 25% inhibition concentration. An estimation ofthe concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC 25 ) Warning Limits (mean IC 25 +/- SAIO or SA 75 ) Page 72. of 9~ontrol Limits (mean rc25 +/- s A25' s A90' or 2 Standard Deviations)
""d ~
(JQ ('D
-...l w
Pimephales promelas Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc. State and USEPA Laboratory Laboratory (IS EPA liS EPA Test number Test date 7-day IC, 5 CT s Control Limits SA.IO Warning Limits SA., Control Limits SA.7S Warning Limits SA'JU Control J.iulil3 cv (&'LKCI) (g/LKCI) CT-2S CT+ 2S CT- SA.Io CT +SA. In CT- SA.zs CT + SA.2S CT- SA.15 CT + SA.75 l
r- sA.'J!I c..* r -~ s..~,.,u I 01-15-08 0.64 2 01-29-08 0.64 0.64 0.00 0.64 0.64 0.08 0.56 0.71 0.13 0.50 077 0.24 0.39 0.88 0 29 [))) lJ V:! 0 00 3 02-05-08 0.65 0.64 O.DI 0.62 0.66 0.08 0.57 'Q72 0.13 0.51 0.78 0.24 0.40 0.89 0 'LV I) J:> 11-'!3 0 02 4 02-12-08 0.64 0.64 0.01 0.63 0.66 0.08 0.57 Q72 0.13 0.51 0.78 0.24 0.40 0 89 0.2Y (J \") t)l)-i 0 0I 5 02-19-08 0.64 0.64 0.01 0.63 0.66 0.08 0.56 0.72 0.13 0.51 0.78 0.24 0.40 0.88 0.29 (1 ):-, n l)J 0.01 03-04-08 0.71 0.65 0.03 0.59 0.71 0.08 0.57 o.n 0.14 0.52 0.79 0.25 0 40 0.90 u 2~ u jb iJ 95 0.04 7 03-11-08 0.69 0.66 0.03 0.60 072 0.08 0.58 0.~ 0.14 0.52 0.80 0.25 0 41 0.91 0 30 ll _l6 lJ 9) ll 0) 04-15-08 0.59 0.65 0.04 0.58 0.72 0.08 0.57 on 0.14 0.51 0.79 0.25 040 0.90 0.29 () 36 11'1*1 (J 06 9 04-15-08 0.60 0.64 0 04 0.57 0.72 0.08 0.57 0.72 0.14 0.51 0.78 0.24 0.40 0.89 0.2~ () 3.--i 0 I.J) 0 Oo 10 05-06-08 0.62 0.64 0.04 0 57 0.71 0.08 0.56 Q72 0.13 0.51 0.78 0.24 0.40 0 89 0 29 () 35 I) 93 0 06 ll 05-20-08 0.66 0.64 0.03 0.57 0.71 0.08 0.57 0.72 0.14 0.51 0.78 0.24 0.40 0.8~ 0 2~ 0 35 II ~3 0 05 12 06-03-08 0 64 0.64 0.03 0.58 0.71 0.08 0.57 0.72 0.13 0.51 0.78 0.24 0.40 0.89 0.29 II.\) 0. 113 0 0) 13 06-10-08 0 76 0.65 0.04 0.56 0 74 0.08 0.57 Qn 0.14 0.51 0.79 0.25 0.40 0.90 0.:>9 I) .l6 O.VI 0 117 14 08-05-08 0.69 0.65 0.04 0.57 0.74 0.08 0.58 Qn 0.14 0.52 0.79 0.25 0.41 0.90 0 2Y 0.36 \101 0.07 15 08-12-08 0.76 0.66 0.05 0.56 0.76 0.08 0.58 Q~ 0.14 0.52 0.80 0.25 0.41 0.91 lUll lUo 1106 (J (18 16 09-09-08 0.79 0.67 0.06 0.55 0.79 0.08 0.59 Q~ 0.14 0.53 0.81 0.25 0.41 0 92 0.30 03'/ 1197 0 09 17 09-11-08 0.59 0.66 0.06 0.54 0.78 0.08 0.58 Q~ 0.14 0.52 0.80 0.25 0.41 0.92 0.30 017 II% 0.09 18 10-07-08 0.57 0.66 0.06 0.54 0.78 0.08 0.58 0.~ 0.14 0.52 0.80 0.25 0.41 0.91 0.30 0 ..\6 U.'Jb tJ.O~
19 10-14-08 0.63 0.66 0.06 0.54 0.78 0.08 0.58 0.~ 0.14 0.52 0.80 0.25 0.41 0 91 0 30 0.36 0.95 o.M 20 10-28-08 0.79 0.66 0.07 0.53 0.80 0.08 0.58 Q~ 0.14 0.53 0.80 0.25 0.41 0.92 0.30 l) ")'/ 0 911 0.10 Nore: 7-d JC 15 = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. CT = Central tendency (mean IC25 ). S = Standard deviation of the TC21 values. Laboratory Control and Warning Limits Laboratory control and warning limits were established usmg the standard deviation of the IC 25 values corresponding to the lOth and 25th percentile CYs. These ranges are more slringentthJn ihc C<lntrol and WUIIilllg lll>liiS recommended by USEPA for the test method and endpoint. SA.IO = Standard deviation corresponding to the 10'h percentile CY. (SA to= 0.12) s._ 25 = Standard deviation corresponding to the 25"' percentile CV. (S;. 25 = 0.21) USEPA Control and Warning Limits SA.JS = Standard deviation corresponding to the 75"' percentile CV. ( SA.JS = 0.38) SA. 9 o= Standard deviation corresponding to the 90 1h percentile CY. (SA 90 = 0.45) CV = Coefficient of variation of the IC 25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH Orgam.\ms obtamedfrom Aquatox, lnc.
.11*1, !ll-21::*DH
Precision of Endpoint }leasurements Pimephales promelas Chronic Reference Toxicant Data
\ "" Environmental Testing Solutions, Inc.
Control Control Mean Test number Test date Survival Growth CT cv CT MSD PMSD CT for Control Growth for Control (%) (mg/larvae) (%) (%) forPMSD (%) (mg/larvae) Growth CV (%) 01-15-08 100 0.842 10.5 0.14 17.0 2 01-29-08 97.5 0.728 0.785 5.4 8.0 . 0.07 9.0 13.0 3 02-05-08 100 0.818 0.796 7.2 7.7 0.07 9.1 11.7 4 02-12-08 100 0.709 0.774 2.4 6.4 0.05 6.8 10.5 5 02-19-08 100 0.833 0.786 6.1 6.3 0.13 15.5 11.5 6 03-04-08 100 0.731 0.777 6.0 6.3 0.07 9.0 11.1 7 03-11-08 97.5 0.695 0.765 5.1 6.1 0.07" 10.5 11.0 8 04-15-08 100 0.999 0.794 9.0 6.5 0.11 11.1 11.0 9 04-15-08 100 0.898 0.806 9.1 6.8 0.08 9.1 10.8 10 05-06-08 100 0.857 0.811 4.4 6.5 0.16 18.2 ll.5 11 05-20-08 100 0.844 0.814 19.2 7.7 0.16 18.8 12.2 12 06-03-08 100 0.918 0.823 6.1 7.5 0.09 9.4 12.0 13 06-10-08 97.5 0.724 0.815 10.9 7.8 0.11 15.7 12.2 14 08-05-08 100 0.854 0.818 15.3 8.3 0.13 15.0 12.4 15 08-12-08 100 0.674 0.808 5.3 8.1 0.07 9.8 12.3 16 09-09-08 100 0.710 0.802 11.1 8.3 0.12 17.3 12.6 17 09-11-08 100 0.824 0.803 11.1 8.5 0.11 12.9 12.6 18 10-07-08 100 0.788 0.802 1.8 8.1 0.10 12.9 12.6 19 10-14-08 100 0.740 0.799 3.2 7.9 0.05 7.2 12.3 20 10-28-08 100 0.586 0.788 6.5 7.8 0.10 17.8 12.6 Note: CV = Coefficient of variation for control growth. Lower CV bound determined by USEPA (IO'h percentile)= 3.5%. Upper CV bound determined by USEPA (90th percentile)= 20% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10'" percentile) = 12%. Upper PMSD bound determined by USEPA (901h percentile)= 30%. CT = Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200la, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effiuent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtained from Aqua/ox, Inc. atoxl0-28-08 Page 74 of96
Pimephales pro melas Chronic Reference Toxicant Control Chart Precision of Endpoint 1\tleasurements
,..,.. Environmental Testing Solutions, inc.
Organism Source: Aquatox. Inc. 1.25 .- Q) 1.00
..... ~ ~
v - 0 1-
~ -----
1-
-..... -....~
0.75
*-*--....= 0.50 0
1-u
=
0 bll
..._,s 0.25 **-* - * * - * * - --*
USEPA Acceptance Criteria(> 0.25 mg per surviving larvae)
~
0 30
= ..:: Kentucky Acceptance Limit(< 30.0%)
0
*.;: ~ ~ 0 *:ell 0 1-20 -= -=
0 1- **-. 0 .. . **- 0 10
.~ u 1-s"'Q) .8 0 0 u
30 US EPA Upper PMSD Bolilld (90th percentile < 30.0%)
~
0
'-' 20 --------- .... ~
lf.J.
~ ......__ ......__
A.. 10 Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Page 75 of 96:ontrol Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)
I I ~:'JLiS,;u.m Ch!oridc Ch;-on:t.: Refuencc To:xican: (EP.-\-821-R-02-013 .\tlethod l!JU*),,)\
.;;,t Species: Pimepha!es promefas I PpKCICR Test ~umber: I C..'2-I Dilutionpreparation information: Comments:
KCI CHM number: C.l-\H ~~'\ 50 g KCJ/L Dissolve 50 g KCl in 1-L I Stock preparation: Dilution prep (mgfL) 450 Deionized water 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 I Diluent volume (mL) Total volume (mL) 991 1000 988 1000 985 1000 982 1000 979 1000 I Test organism information: Organism age: ?.S 1-\ou~c::. aLb Test information: Randomizing template: UI"J ,._~ Date and times organisms Incubator number and IC* 21*0A 1\oOO ob I were born between: Organism source: ~ ~~ PP lO*t1*Gf oc shelf location: Artemia lot number: &1co~1~ Transfer bowl information: pH= SU Temperature= Total drying time: l.'-'*Holl~ I Average transfer volume:
0. \~tl.\.-Q.
1.'-IA Date I Time in: l\*()~'"()t Date I Time out: ll*t6*o& llo '1.0
*~ "2.b Oven tem_E_erature: bo'C I Daily feeding and renewal information:
I Day Date Morning feeding Afternoon feeding time Test initiation, MHS batch used Analyst 0 I 2 I 3 4 5 6 7 Control information: Acceptance critena Summary of test endpoints:
%Mortality: 07. ~20% 7-day LC 50 s,,,t>
Average weight per initial larvae: c.Sib r~*%_~ ,~, .~~.fi':t,tl~G\f,i:;;,iz:,c~; ,"f.(;?;~~ NOEC 1C:::.~ Average weight per surviving larvae: O*S'Io ;::>: 0.25 mg/larvae LOEC qoo ChV 8'2.\. (&. IC2s '1U.. 8'
**~**~
I I Species: rmepha!:c: promelas I Survival a n d Grow tll D at a Day Control 450 mg KCI/L 600 mg KCI/L I 0 A B c D E F G H r J K _!:_ ID /0 Ia IO /0 IO IO lb Jo IO 10 IO I 1 {0 10 10 IO tc IO 10 /0 to IO /0 10 2 lO /0 10 10 10 10 to I(J 10 10 10 I 3
/0 10 /D /D JO ID 10 IO 10 /tJ 10 /0 q'rA. ~"'1(.
I 4 5 to /\) lb /0 f(j ID /0 10 /0 10 ~ to JO J0 /0 JO 10 IO /0 10 /0 B'J.. <\ I 6 10 ID 10 /Q /D 10 q*c.\ IC /0 /0 g 'i 7
/{) /D It> ID to /D q I0 IO 10 g t:t\l..'r I A= Pan weight (mg)
Tray color code:: d.~t<RN 1'-t. '-l'i !'l. 01 Analyst: t .e-0 ll{:l'C tl\.3~ 1'-\.\ft. \3.tl3 3.'03 1\~.\l \'\ .a1 13.0~ \3.01.. 13.SG I B =Pan+ Larvae weight (mg) i."
-Lu!S 1
.

;:z.J z..z; .*~o i~.ft: 20.bD ~ ~. c b 'S.lvl I , ...
Analyst: ~LS 'Z.t.t-\ \C'l' w'jl /i~~) 1S.lc l j r:, \J ""i I C =Larvae weight (mg)
=A-B "S.13 ()Vi ~**n. G.\*~ s*:;-z. 5 l l S. "lb ') ~.1 C).lc l s L.Jl L1.'L) c.L~~
I Weight per initial number
,*1*.\J 2 lc:,,(' .......,t.)
of larvae (mg) 1"-\3 i/i:i'\ !1),\:;j.l. \,.i*" 11.-*<;'1' IP . 0) <571 ic.S78 0?~.:,\ lce\.;1 1.)<1 l',. ' .J.i;'"cl
~* ) '-'* = C I Initial number of larvae c~? \;'.
I Average Percent weight per reduction O.Siol 3.1.-1~ o.S'-\\ lo .I 7o.l initial from control o.SR!o I number of larvae (mg) (%) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Calculations and data reviewed: ~ Comments:
I I I Spt:(ies: Pimephalt!-,- prameias I Survival and Growth Data I Day M 750 m~ KCI/L N 0 p Q 900 mo KCI/L R s T u 1050 m KCI/L v w X 0 JD /0 /t) /{) /(J /{) to ID It; ID /0 IO I 1 IO /0 q'c4. '\,cA. ~
*cl ~,( ., lJ..
(Q 4J.: ~~ s~ s s~
'-\
1,10\ I 2 JD /D &'J.. q [! .., ,cl l (., L.\ ~ 'S '-\ 3
/C 10 & q & .., Ia ,tA ~ '-\ 'S $ ~*G4.
I 4
/c /0 8" & ,J. ~.,_~ ., ~ s'~ 3'~ 5 4..J. 3 5
tO 10 .f ...t "l,il \~ 5
.~
5 2-
)fA 3l.IA ~ ,_leA I 6 to 10 1 ' .~ & '-\ ~
lo s s 1... 1.'" 4 1
'c.\
7
"'td .., i.( JJs \S~
s ~~ \~
\s""
I A= Pan weight (m~. f() t..\ 'S "2.. 3 Tray color code:: .QYe.ei'J tUl'L \'\. '\ '0 13.'\(p 1'\.'~"' l'\..~'0 13. te't ~ l3.':>lj !t.l..t3 lt.f.S3 t'f .00 {Ll-t'-f
~ ~
I Analyst: f'-{.S9. B =Pan + Larvae weight (mg) Analyst: ~~~
.2 il.l.o\ 2..t2') i0*\p l M1\""' i~.t.1C: 'i,C::i ,.._ ... ) ! \l.l\~ *S *'* ---ti i LJ.t-l IS.51 *~"'5'1 I *.J. ' 11..\!5[
I 1..- *.} 1
\J, I ~-J 1..*
C =Larvae weight (mg) Y.lc\ fn L.! '"\.1 \ I~ LCc ('¢ --..cc as-! I -,,-*
=A-B \.1(* l c-_>l 0.l~,li ! ~,....~ 1 I.~ I f.S'-1 c~ ~'
I Weight per initial number a.t.S'1
~ ... c\ c(;'1"1 ,~, .V; )-l ...v*'-\'\\ ,.._*-ztt: .- 7_ 3iJ c.\':)'-1 of larvae (mg) ,,*...\'0\ \.i* lJ* ,\
7l,:-t
.h.* \::.6"1 \..* ' o.c;.~tf \.).\ i ~ :'\c:\ = C I Initial number of larvae \,.,*
I Average weight per Percent reduction initial from control o.sc~ l~.\7.. 0 :z.?,~ sa..£7. o. cf\ \ &4*~7. I number of larvae (mg) (%) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk = sick, sm = unusually small, I lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed:.&__ I Comments: I ~Page 78 of 96
""Q ~ ~
tl)
-....) \C Q \C C\
Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method l 000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
) Environmental Testing Solutions, Inc Test numbt!r:
Test dates: Octohn :!H PpKC\('R #162 1\,'ovembt:r 0--1 1 200~ Cunccotrl:ltiun (mg/L Rcplic~&tt! Fin:tln,umbcr of Juiliwl number uf A"' Pu weight. (mg) B- P:~.n + Lllrv:.iC Lun'II.C weight (mg) Wcigbt I Survil'inR Mcun "*ei~btl Ct)(ffidtnt of\ ariatiou Wcigbt f Initial number Mclin sun*ival Mc:.~.n \\Cii-:1111 Juiti;tl ( 11\:nidcnt 11f Pcr(l'l\1 r4.'ilurtiou from KCI) tan**c han*ae wcigbt(mg) ~A-B oumbtr of larvae (mg) Surviviog number of (Mnn"C'IthlpcrlurYJ>'i.rll. nf l~&rvll.e (mg) (%) number or I <In .a: \ a 1 i ~1l11m ( *~ ~ J ron tl nl (*~*i. ) nu*n'louuCllonllf) (*/G) lllJ"V.IIe(mg) lm~o:) A )0 10 14.78 20.21 5.43 0.543 0.543 B 10 10 14.36 20.00 5.64 0.564 0.564 Control 0.586 65 100.0 0.586 6.5 ~~ot :.~pplital>le c 10 10 14.62 20.84 6.22 0.622 0.622 D 10 10 13.28 19.41 6.13 0.613 0.613 . E 10 10 13.83 19.35 5.52 0.552 0.552 F 10 10 14.11 19.88 5.77 0.577 0.577 450 0.583 7.0 97.5 0.567 !.2. 3.2 G 10 9 14.82 20.60 5.78 0.642 0.578 H 10 10 13 06 18.67 5.61 0.561 0.561 I 10 10 13.02 18.69 5.67 0.567 0.567 J 10 10 13.59 19.06 5.47 0.547 0.547 600 0.591 14.9 92.5 0.547 17.1 fl.7 K 10 8 14.44 18.67 4.23 0.529 0.423 L 10 9 14.02 20.51 6.49 0.721 0.649 M 10 9 12.82 17.61 4.79 0.532 0.479
-----~-*-- **-**-*------
N 10 10 14.98 21.25 6.27 0.627 0.627 750 0.622 10.1 82.5 0.509 I :"I.) U.l 0 10 7 13.96 18.67 471 0.673 0.471 p 10 7 14.46 19.05 4.59 0.656 0.459 Q 10 4 14.38 16.38 2.00 0.500 0.200 _"_'__~ R 10 5 13.89 16.57 2.68 0.536 0.268 900 0.503 6.4 47.5 0.239 S9.2 s 10 5 14.59 17.16 2.57 0.514 0.257 T 10 5 13.54 15.84 2.30 0.460 0 230 u 10 I 14.23 14.87 0.64 0.640 0.064 1050 v 10 2 14.53 15.54 101 0.505 0.525 15.9 0.101 17.5 0.091 SJ.J I H-L:"' w 10 3 14.00 15.54 1.54 0.513 0.154 X 10 1 14.14 14.58 0.44 0.440 0.044 I Dunnett's MSD value: 0.1043 MSD= Minimum Significant Difference PMSD: 17.8 PMSD= Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared stati~tically signiflt:anlln 11 whl\k: clfhn:llltuxJcily lt.!si Lower PMSD bound detennined by US EPA (!Otl1 percentile) = 12%. Upper PMSD bound detennined by USEPA (90th percentile)= 30%. Lower and upper PMSD bounds were detennined from the lOth and 90th percentile, respectively, of PMSD data from EPA's WET lntorlaboratory Va~iabrl11y ~l11riy ( lJ~I'I'A. 2\IU 1"* ( ISEI' A, 201J I hl USEPA. 2001 a, 2001 b. Final Report: lnterlaboratOJy Variability Study of EPA Short-tenn Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix EPA-821-B-0 1-004 and EPA-82 1-ll-01-005. US Environmental Prot<ctiuo A~<IIL) 1 "'""'""* 1lll Organisms ohtainedfrum Aquatox, Jm:. atu-.: I0-2X (J~
""C ~
fTC~
!'!)
00 c Statistical Analyses e
~ ~~ Environmental Testing Solutlonl, Inc.
Q\ Larval Fish Growth and Survival Test-7 Dax Survival Start Date: 10/2812008 Test ID: PpKCICR Sample ID: REF-RefToxicant End Date: 1114/2008 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mg/L D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 0.9000 1.0000 600 1.0000 1.0000 0.8000 0.9000 750 0.9000 1.0000 0.7000 0.7000 900 0.4000 0.5000 0.5000 0.5000 1050 0.1000 0.2000 0.3000 0.1000 Transfonn: Arcsin Sguare Root Rank !-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 () 40 450 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 16.00 10.00 I 40 600 0.9250 0.9250 1.2951 1.1071 1.4120 11.347 4 14.00 10.00 40 750 0.8250 0.8250 1.1608 0.9912 1.4120 17.825 4 12.00 10.00 7 40
'900 0.4750 0.4750 0.7602 0.6847 0.7854 6.622 4 10.00 10 00 21 4lJ '1050 0.1750 0.1750 0.4217 0.3218 0.5796 29.582 4 10.00 10 00 33 .Jll Auxtliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01) 0.96270359 0.884 0.12251881 0.07880166 Equality of variance cannot be confinned Hypothesis Test (!-tail, 0.05) NOEC LOEC ChV TV Steel's Many-One Rank Test 7 50 900 821.583836 Treatments vs 0-Control Maximtun Likelihood-Probit Parameter Value SE 95% Fiducial Limits Control Chi-SS Critical P-value Mu Sigma lter Slope 8.99516854 1.26542412 6.51493721 11.4753999 0 6.5280808 7.81472778 0.09 2.94300923 0.111 J 7079 4 Intercept -21.472864 3.69736461 -28.719699 -14.226029 TSCR Point Probits mg/L 95% Fiducial Limits ECOl 2.674 483.48978 389.172926 549.252679 EC05 3.355 575 63813 493.057452 632.425785 ECIO 3. 718 631.739647 558.367087 682.984696 EC15 3.964 672.648407 606.421898 720 344523 EC20 4.158 707.042528 646.71135 752.4602 EC25 4.326 737.947854 682.481552 782.205752 EC40 4.747 821.948177 774.797303 870.093762 EC50 5.000 877.019469 829.55876 935.129 EC60 5.253 935.780601 883.109836 1010.80758 EC75 5.674 I 042.30013 971.791044 1159.98544 EC80 5.842 1087.85981 1007.70304 1227.18709 EC85 6 036 J 143.48471 1050.48901 1311.38658 EC90 6.282 1217.53181 1106.07161 1426.66922 EC95 6.645 1336.1921 1192.70601 1618.06819 EC99 7.326 1590.85723 1371.48327 2052.73137 Organisms obtained from Aquatox, Inc. at ox I U _>X OR
~ ~
I1Q ('!) QO 0 Statistical Analyses I,C 0'\
,,;,e~ Environmental Testing Solutions, Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: l 0/28/2008 Test JD: PpKClCR Sample JD: REF-Ref Toxi cant End Date: ll/4/2008 Lab ID: ETS-Envir. Testing SoL Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mg/L 1 2 3 4
- ---~--------- ..
D-Control 0.5430 0.5640 0.6220 0.6130 450 0.5520 0.5770 0.5780 0.5610 600 0.5670 0.5470 0.4230 0.6490 750 0.4790 0.6270 0.4710 0.4590 900 0.2000 0.2680 0.2570 0.2300 1050 0.0640 0,1010 0,1540 0.0440 Transform: Untransformed !-Tailed lsotllllic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-M<:UII D-Control 0.5855 1.0000 0.5855 0.5430 0.6220 6.509 4 (),5855 I .OOflO 450 0.5670 0.9684 0.5670 0.5520 0.5780 2.236 4 0.406 2.290 0.1043 0.5670 O.lJ684 600 0.5465 0.9334 0.5465 0.4230 0.6490 17.093 4 0.856 2.290 0.1043 0.5465 0.'!.l34 750 0.5090 0.8693 0.5090 0.4590 0.6270 15.539 4 1.680 2.290 0.1043 0.5090 O.X693 900 0.2388 0.4078 0.2388 0.2000 0.2680 12.720 4 0.2388 0.4()'JX 1050 0.0907 0.1550 0.0907 0.0440 0.1540 53.254 4 0.0907 (1.155() Auxiliary Tests Statistic Critical Skew Kiirt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94647986 0.844 0.24683066 I .28466:l~ I Bartlett's Test indicates equal variances (p = 0.04) 8.28880119 11.3448668 Hypothesis Test (1-tail, 0 05) NOEC LOEC ChY TU MSDu MSDp MSB MSE F-Prob df' Dunnett's Test 750 >750 0.1042998 0.178138 0.004302 0.00414883 0.41126868 3. 12 Treatments vs D-Control Linear Interpolation (200 Resamples) Point m~L SD 95% CL(ExE) Skew
-:-:-::~----=--=-:--:--:---'~'-"-:-::-:-::-:--.:.....;.,---::--=-:-:----------------------------**------**-*-* ---- !COS 528.84 118.50 171.14 891.05 -0.2714 ICJO 678.20 81.73 406.86 823.15 -0.4723 IC15 756.29 53.17 473.75 798.03 -1.6249 IC20 772.53 22.83 690.60 810.16 -3.1449 IC25 788.78 13.72 744.57 822.58 -0.0595 IC40 837.53 9.24 808.29 860.09 -0.0603 ICSO 870.03 7.66 845.69 890.11 -0.0839 Organisms obtained from Aquatox, Inc. at ox I 0-2~-08
*~ > '*:\' '~>)t~~,*_.~-
Page 1 ;f) Spe~ies: Pimep:ut!es prvmeias I 1050 mg KCI!L STOCK Page 82 of96
Species: Pimeph,ues promelas 450 mgKCl/L 1050 mg KCI/L STOCK Page 83 of96
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Environmental T~sting Solutions, Inc. 1.14 f .. USEPA Control Limits (+/- 2 Standard Deviations) 1.12 1-1.10 1.08 --- 1.06 1.04 1.02 2.5
-z u ~ 2.0 USEPA Warning and Control Limits (75th and 9dh Percentile CVs) ~
bJ:) on N 1.5
* * * * * -.= * * * * * * * * *
u
~
1.0 C'l:
"t:SI 0.5 r-Laboratory Warning and Control Limits (1 dh and 25th Percentile CVs) 1.3 1.2 1.1 1.0 0.9 Test date 7-day IC 25 = 25% inhibition concentration. An estimation ofthe concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. - - - Central Tendency (mean IC 25 ) - - - Warning Limits (mean IC25 +/- SA 10 or SA 75 )
Page.84 of ~ontrol Limits (mean IC 25 +/- SA 25 , SA. 90 , or 2 Standard Deviations)
'"d ~ ~
t'D 00 Ul 4\b
~
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart
) Environmental Testing Solutions, Inc.
State and USEPA Laboratory Laboratory USEPA lJS],;l'.\. Test number Test date 7-day JC 25 CT s Control Limits s.._, 0 Warning Limits SA.lS Control Limits SA.7S Warning Limits SA.9U Coutrull.imits cv (g/L NaCI) (g!LNaCI) CT- 28 CT+2S CT- SA.lO CT + SA.JO CT - S.._,s CT + S.._,s CT- SA.1s CT + SA.7s CT- SA.Yil ( T I sA. Oil I 05-08-07 1.10 2 06-05-07 ].]0 1 10 0.00 110 1.10 0.09 1.01 1.18 0.19 0.91 1.28 0.49 0.60 1.59 0.68 ll-12 I 78 0 00 3 07-10-07 1.06 1.08 0.02 104 1.13 0.09 1.00 I 17 0.18 0.90 1.27 0.49 0.60 1.57 0 67 0.41 I /':i 0 02 4 08-07-07 1.10 1.09 0.02 1.05 ].]3 0.09 1.00 1.17 0.18 0.90 127 0.49 0.60 1.58 0.67 0 ~I I 76 0.02 5 09-05-07 1.08 109 0.02 1.05 1.12 0.09 1.00 1.17 0.18 0.90 1.27 0.49 0.60 1.57 0.67 U-11 I /6 0 02 6 10-02-07 106 108 0.02 104 ].]2 0 09 1.00 1.17 0.18 0.90 1.27 0.49 0.60 1.57 0.67 041 I /'5 0.02 7 1 J-06-07 1.06 1.08 0.02 1.04 1.12 0.09 ~~ 1.16 0.18 0.90 1.26 0.49 0.59 1.56 0.67 041 /) 0.02 8 11-06-07 1.07 1.08 0.02 104 1.11 0.09 ~~ 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 l!-11 IS 002 9 12-04-07 1.07 1.08 0.02 1.04 Ill 0.09 0.~ 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.<1 1 n 0.02 10 OJ 08 109 1.08 0.02 1.05 1.11 0.09 ~~ 1.16 0.18 0.90 1.26 0.49 0.59 1.56 0.67 OAI h 0.02 II 01-08-08 1.09 1.08 0.02 1.05 1. 11 0.09 ~~ 1.17 0.18 0.90 126 0.49 0.59 1.57 0.67 () 41 IS (){) 1 12 02-05-08 1.07 1.08 0.02 1.05 1.1 1 0.09 ~~ ].]7 0.18 0.90 1.26 0.49 0.59 156 0.67 0.'11 I /) 0 OJ 13 03-04-08 1.07 1.08 0.02 1.05 I. II 0.09 0.~ 1.16 0.18 0.89 1.26 0.49 0.59 1.56 0.67 0 41 l.h 0.01 14 04-08-08 107 1.08 0.01 1.05 ].]1 0.09 0~ 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 041 lh () 01 15 05-06-08 106 108 0.01 1.05 1.11 0.09 ~~ 116 0.18 0.89 126 0.48 0.59 1.56 0.67 l) 41 I '/4 0 01 16 06-03-08 1 11 108 0.02 1.05 Ill 0.09 ~~ 1.16 0.18 0.89 1.26 0.49 0.59 1.56 0.67 () ~ 1 I 75 0 02 17 07-08-08 1.07 1.08 0.02 1.05 1.11 0.09 ~~ 1 16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 I 7) 0 01 18 08-05-08 109 1.08 0.02 1.05 Ill 0.09 0.~ 1J6 0.18 0.90 1.26 0.49 0.59 156 0.67 0.41 I 75 0.01 19 09-09-08 1.10 1.08 0.02 105 1.11 0.09 ~~ 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 041 I /) 0 Ul 20 10-07-08 1.09 1.08 0.02 1.05 1.11 0.09 ~~ 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 I '!) U.UI Note: 7-d IC 25 = 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC25 ). S = Standard deviation of the IC 25 values. Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the 1C25 values corresponding to the 1Oth and 25th percentile CVs. These ranges are more st1 ingcntthan the> cotllrul and warning limits recommended by USEPA for the test method and endpoint. 8.._ 10 = Standard deviation corresponding to the lOth percentile CV. (SAw= 0.08) 8.._ 25 = Standard deviation corresponding to the 25th percentile CV. (SA 15 = 0.17) USEPA Control and Warning Umits 8.._ 75 = Standard deviation corresponding to the 75th percentile CV (SA 75 = 0.45) S.._ 90 = Standard deviation corresponding to the 90th percentile CV. (SA'"= 0 62) CV = Coefficient of variation of the IC 25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effiuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Pruteciion Agency, Cincinnati, OH
/II 11~ 08
Precision of Endpoint IVIeasurements Ceriodaphnia dubia Chronic Reference Toxicant Data
'\ ,iJ,¥,!1' Environmental Testing Solutions, Inc.
Test Control Control Mean number Test date Survival Reproduction CT cv CT MSD PMSD CT for Control Mean for Control (%) (offspring/female) Reproduction (%) Reproduction (%) forPMSD (%) (offspring/female) CV(%) 1 05-08-07 100 32.0 8.5 2.7 8.4 2 06-05-07 100 28.4 30.2 7.5 8.0 2.5 8.7 8.6 3 07-10-07 100 29.3 29.9 6.0 7.3 2.5 8.5 8.5 4 08-07-07 100 28.7 29.6 7.5 7.4 2.9 10.0 8.9 5 09-05-07 100 29.7 29.6 5.7 7.0 2.5 8.4 8.8 6 10-02-07 100 29.5 29.6 9.4 7.4 2.4 8.2 8.7 7 11-06-07 100 28.0 29.4 6.1 7.2 2.5 8.8 8.7 8 11-06-07 100 30.4 29.5 5.6 7.0 2.2 7.2 8.5 9 12-04-07 100 31.8 29.8 5.5 6.9 2.5 7.9 8.5 10 01-08-08 100 30.3 29.8 6.6 6.8 2.2 7.3 8.3 11 01-08-08 100 31.8 30.0 4.9 6.7 2.4 7.7 8.3 12 02-05-08 100 31.3 30.1 6.4 6.6 2.8 9.1 8.3 13 03-04-08 100 31.5 30.2 8.0 6.7 2.7 8.5 8.4 14 04-08-08 100 29.6 30.2 7.7 6.8 2.6 8.8 8.4 15 05-06-08 100 32.1 30.3 6.3 6.8 2.5 7.9 8.4 16 06-03-08 100 30.5 30.3 7.9 6.8 3.0 9.9 8.5 17 07-08-08 100 30.9 30.3 6.7 6.8 2.4 7.7 8.4 18 08-05-08 100 29.4 30.3 7.9 6.9 2.2 7.6 8.4 19 09-09-08 100 28.3 30.2 8.5 7.0 3.0 10.5 8.5 20 10-07-08 100 32.4 30.3 7.2 7.0 2.7 8.4 8.5 Note: CV = Coefficient of variation for control reproduction. Lower CV bound determined by USEPA (IO'h percentile)= 8.9%. Upper CV bound determined by USEPA (90'h percentile)= 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (lO'h percentile) = 13%. Upper PMSD bound determined by USEPA (90'h percentile)= 47%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA 2001 a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. 10-07-08 Page 86 of96
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint :Nleasurements
'\. ,.,. Environmental Testing Solutions, Inc.
c: 35 0
~ ~ *********** .... =E ~ ~
0
...C.. ~ ~
OJ) c: 30 25
~
0 c: _.,
~ .0....
0 20 0 u 15 Minimum Acceptance Criteria(> 15.0 offspring per surviving female)
-= .:z... ~ ~
c: 40 North Carolina Acceptance Limit(< 40.0%)
... =
0 Col
."E ~
t~
~ 0..
30 Kentuck')' Acceptance Limit(< 30.0%)
= --
0 0 20
*c::
Q,l = 0 s u 10
~
0 ~ u 50
. ili USEPA Upper PMSD Bound (90 percentile< 47.0%)
40 30 20 10 Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD) Page 87*of ~ontrol Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
Page l of 6
'------ + --------- ~ On,orOf\metu~l'!!lllnqSoh**'Of'f. irx.
Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 :VIethod 1002.0) Species: Ceriodaphnia dubia CdNaCLCR #: 4'0 Dilution preparation information: Comments: NaCI CHM number* (..t-\~ \"2..0 Stock preparation: 100 g NaCI/1 (dissolve 50 g NaCI in 500 ml deionized water) Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test orf{anism source information: Test information: Organism age: < 24-hours old Randomizina template color: 6UJe. Date and times organisms were born 10*04.*0& *s'-4'2.-ru n~s Incubator number and shelf between: location: l.C. \ Culture board: 0'\*~o-~ A Replicate number: I l 2 I JJ 4 l 5 I 6J 7 I8l 9 I 10 YWT batch: Culture board cup number: \ 11.. I .?I I 5 I 8 I '\ I t \ II'- I ,, I '1.4 O'i*0'\*0~ Transfer bowl information: pH=1.&& su Temperature = '1-\.'\ oc Selenastrum batch: 0 "*2.1:\-<:) f Daily renewal information: Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0 2 3 4 5 6 7 Control information: Acceptance criteria SummaryoLtest endpoints:
% ofMale Adults: o7.. s20% 7-day LC 5o ) 140() % Adults having 3rd Broods: ltx/1. ~ 80% NOEC /OOD % Mortality: (j{. s20% LOEC rz.__oo Mean Offspring/Female: .32."' ~ 15.0 offspring/female ChV ICl .. ~ .'-\ %CV: 1* 2 "7~ <40.0% IC2s 1o'\ ~.~
Page 88 of96
I Page 2 -:f 6 I Cd"'aCLCR :;.: 8'0 Species: Ceriodaphnia dubia --=--- CONTROL Survival and Reproduction Data Replicate number Dav 1 2 I 3 4 5 6 7 8 9 10 I Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality \._
'--- L L L- L L L L ~
2 Young produced 0 0 0 0 0, 0 0 0 c () Adult mortality L L L- L L L L L L '- 3 Young produced () 0 0 0 0 0 0 0 0 0
\.._ \..._
Adult mortality L L \.._ \._ \.._ L. '--- \. 4 Young produced s s 1..\ '-\ ~ s '-\ ~ s 5 Adult mortality L L \.... \.... \.._ L- L \.._ \....... '-- s '- 1£.. l!. '~ I'"Z.. Young produced
\ \._ '~ 'C> -'L t\ JC Adult mortality L L L L. \,_ \..._ '- \_.
6 Young produced \\ \a t4 l'-t l"'\ \lo \& 's - 1\o Adult mortality c.._ L L \..._ \_ L \..__ L '- '- 7 Young produced Total young produced
.3'-\ .3'- 28 o\ ~\ 35 3~ 32.. 3\ !10 L..
Final Adult Mortality L- ~ '- ~
'- L- '- \....- L_
X for 3'd Broods )<.. )<... ~ 7< '>"-- )<._ ><- ~ r:- ><- Note. Adult mortahty (L = hve, D =dead), SB =split brood (smgle brood split between two days), CO= carry over (offspnng carried over With adult during transfer). Concentration:
% Mortality: I o1.
Mean Offspring/Female: J o2A 600 mg NCVL a Survzva. I an dR epro d uctzon D ata Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0
\_
0 0 a C) 0 n 0 a 0 Adult mortality
'- L '-- '- \...... '-- L '-- '--
2 Young produced 0 () 0 0 0 0 0 0 0 0 Adult mortality L L L. L L L L '- L- '- 3 Young produced b 0 () 0 () a 0 ()_ 0 0
\._ L L Adult mortality L L L L- L '- \...
4 Young produced lo s 5 \c 1...\ s s -~- '-'. s Adult mortality L L L L L L L L L L 5 Young produced t"-\ \"2- ID \ "'L \~ 1\ l~ \2 \ "'2.. 10 6 Adult mortality Young produced Adult mortality It L L. L. IS L L Jl..\ L L IS L.. L
\...._
L It..\ L. L llo L.. L L I_(Q L.. II. L L L-iJ 7 Young produced Total young produced 3~ 3'Z. "Z'1 3~ ..3Y ..3b 34 ..32 32 3L Final Adult Mortality l_. L \. L L L L '- L '- Note. Adult mortality (L =live, D =dead), SB =split brood (single brood split between two days). CO= carry over (offspnng carried over with adult during transfer). Concentration:
% Mortality: 07 ..
Mean Offspring/Female: at.." Page 89 of96 %Reduction from Control: -o.<.,7p
Page 3 of 6 Species: Ceriodaphnia dubia CdNaCLCR =t: Eo 800 mg NaCl/L Survival and Reproduction Data Replicate number Day 1 2 I i 3 i 4 5 6 7 8 9 10 1 Young produced 0 0 I 0 D 0 0 0 0 0 n Adult mortality L L '- L '- l- '- \.__ L L.. 2 Young produced 0 0 0 0 0 Q_ _0_ 0 () t> Adult mortality L L \..__ L- '-- '- L L L. L 3 0 Young produced 0 0 0 () 0 0 D 0 0 Adult mortality L \...._ L L L.. L \...._ L- L L 4 Young produced '4 s s '-\ '-\ ~ Ia s s s Adult mortality L- \.._ L.. L L.. L.. L '- '- '- 5 I\ Young produced IY
*~L.. \ 10 \L. l"l. 12.... *~ \ \'1...
6 Adult mortality Young produced L ll ,,L L
\.._
l i L l(o 1'-l L. 1"-\ L..
\q \._
L L
\~
L
,, L..
1"4 Adult mortality \..- '-- L. \..._ \.._ 7 Young produced Total young produced J
~"-\ ..3\c. 3'-\ 3o 3\ .:!,C 3'l. 31. ~\..\ 3()
Final Adult Mortality L L L. '- .~ L \.. \..__ \..._ L.:_ Note: Adult mortahty (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offsprmg carried over with adult during transfer). Concentration:
% Mortality: Oi Mean Offspring/Female: 3"1..:, % Reduction from Control: 0*~7 1000 mg NCl/L a Survzva. I an dR epro duct'zon D ata Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 a 0 0 Q D n _Q_ () ()
Adult mortality L l L L.. \..._ L- '-- \.._ \.__ L. 2 Young produced (') 0 0 0 0 0 0 0 0 0 Adult mortality L L \..._ L L- L L L L L 3 Young produced () 0 D D 0 6 a 0 0 a Adult mortality L.. L L L L- '- L '- L... L_ 4 Young produced '-\ s -s \o '-\ s '5 4 '-l l..:, Adult mortality L L L '-- '-- L L L_ \..._ \..... 5 Young produced ~~ IC \'2. tO I l 1¢ l& IL... IC IQ Adult mortality L L L L L L L L L '- 6 Young produced ~~ \$ 1'5 L"-\ *1 l'-\ lq llo ,-; \~ Adult mortality \__ L L.. L L... L L L L L-7 Young produced ~ Total young produced ~0 3'2 3-b 2>2. 31.. 32 32,. 2'\ 2'\
.30 Final Adult Mortality L '- L L \..._ L... '-- ~ L '-
Note.* Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood split between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration:
% Mortality: 01.
Mean Offspring/Female: ~.8
%Reduction from Control: c.v~7b Page 90 of96
I Page 4 or* 6 I CdNaCLCR#: --~6~0~* I Species: Ceriodaphnia dubia POO mg.~ a Cl/L Survival an dR epro d uctzon D at a Replicate number I Day 1 Young produced 1 0 2 D 3 0 0 4 0 5 0 6 7 0 0 8 0 9 0 10 Adult mortality L '-- L- L- L- L- "-- '-- L L_ I 2 Young produced Adult mortality 0 L. L () a 0 L 0 _Q_ L.. 0 L 0
'-- L Q 0 l.,_
3 Young produced 0 0 0 C) 0 0 c 0 6 () I 4 Adult mortality Young produced
\._
I.{ '4
~ \._ '-\
L... _S_ L_
--':l 4 \.__
4 l.-
.3 L \.._. '-\
I 5 Adult mortality Young produced l L '- '--
'5 \.._
I D
\......
lD L
'-\ ,'- ~
L. L lC L 6 Adult mortality. Young produced ID L
""0 L
s
\..._
s 8 L s 0
\.._
IC L 5
~
d I 7 Adult mortality Young produced
'- '--- \_ L \_ '--- L \_ L \..__
Total young produced 2\ 13 1.:!> L'\ t'\ 13 I \ 2~ 1.& "2.\ Final Adult Mortality \,. \._ '-- '- \...... L. '-- '-- \.._. \. Note: Adult mortality (L =live, D =dead), SB =split brood (smgle brood spilt between two days), CO= carry over (offspnng carried over with adult during transfer). Concentration:
%Mortality: ()1.
Mean Offspring/Female:
% Reduction from Control: q,.,.,. \1. \
1400 mg NCl/L a . I an dR epro d uc rwn D a ta Survzva Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 a_ 0 0 0 0 Adult mortality '-- l- \...._:.. L- \._ L__ L- L.. L \.... 2 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L L- L L. L L L... L \...._ 3 Young produced (') 0 0 0 0 0 D 0 0 () Adult mortality \.._ \._ L L L L L L L. l 4 y _'-\ '- '-\ '-\ 4 .3 Young produced 0 ~ '- Adult mortality \..._. L '-- L L- \_ \_
'- L. '-
5 Young produced '-l '- c s \ 3 I q to '- Adult mortality L L L- '- '- \...._ L.. L \.._ \..._ 6 Young produced 0 0 I 0 0 0 0 0 0 ~ Adult mortality \..._ \..... \...._ \_ \_ \._ L L.. '-- L-7 1>4 Young produced v Total young produced
& :s s .& 3 I 3 g /0 10 Final Adult Mortality L L '- L. L L \._ L L L Note: Adult mortahty (L = ltve, D =dead), SB =spilt brood (smgle brood spht between two days), CO= carry over (offsprmg carried over with adult during transfer).
Concentration:
% Mortality: 01.
Mean Offspring/Female: U/.1 Page 91 of 96 %Reduction from Control: 1'f, 3?o
Verification of Ceriodaphnia Reproduction Totals Environmental Testing Solutions, Inc. Control 1000 m!! NaCUL
"d ~
(J(l (">
\C w
0
\C 0\ *** Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control '<) Environmental Testing Solutions, Inc.
Verification of Data Entry, Calculations, and Statistical Analyses Test number: CLINaCICR #80 Test dates: Udobu 07-13,2008
,'),!, ----- ------- \Jj Concentration Replicate number Survival Average reproduction Cueffidenl of Pcn*cnt 1 cUuctiun from "ariutiuu l ~'/1.1) tuulrul CYv)
(mg/L NaCI) (%) (offspring/female) I 2 3 4 5 6 7 8 9 10 Control 34 36 28 31 31 35 33 32 31 33 100 32.4 7.2 Not applicable
----- c---- **-***
600 38 32 29 33 34 30 34 32 32 32 100 32.6 7.5 -0.6 800 34 36 34 30 31 30 32 32 34 30 100 32.3 6.5 (J.J 1000 30 30 32 30 32 32 32 32 29 29 100 30.8 4.3 -l.<J 1200 21 13 13 19 l9 13 II 23 l8 21 100 17.1 2-1.7 -17.2 1400 8 5 5 8 3 7 3 8 10 10 100 6.7 3!L6 '1~.3
'--- ---- ---- --- -- --~
Dunnett's MSD value: 2.711 MSD= Minimum Significant Difference PMSD: 8.4 PMSD= Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be dedarcd staustlcally significant in a whole effluent toxicity test Lower PMSD bound determined by USEPA (!0 1h percentile) = 13%. Upper PMSD bound determined by USEPA (90th percentile)= 47%. Lower and upper PMSD bounds were determined from the I Oth and 90th percentile. respectively, ofPMSD data from EPA's WET lnlcrlabu1 Jlory Variability Study (USEPA, 200 I a; US EPA, 200 I b). USEPA. 200 !a, 200! b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-0 1-004 and FPA-821 B 01-005. US Environmental Protection Agency, Cincinnati, OH.
"ti ~
(JQ
~ \0 0 ...... Statistical Analyses \0 0'1 .J Environmental Testing Solutions, Inc.
Ceriodaphnia Survival and ReEroduction Test-ReEroduction Start Date: 1017/2008 Test ID: CdNaCICR Sample ID: REF-RefToxicant End Date: 10/13/2008 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mg!L I 2 3 4 5 6 7 8 9 10 D-Control 34.000 36.000 28.000 31.000 31.000 35.000 33.000 32.000 31.000 33.000 600 38.000 32.000 29.000 33.000 34.000 30.000 34.000 32.000 32.000 32.000 800 34.000 36.000 34.000 30.000 31.000 30.000 32.000 32.000 34.000 30.000 1000 30.000 30.000 32.000 30.000 32.000 32.000 32.000 32.000 29.000 29.000 1200 21.000 13.000 13.000 19.000 19.000 13.000 11.000 23.000 18.000 21.000 1400 8.000 5.000 5.000 8.000 3.000 7.000 3.000 8.000 10.000 10.000
!-Tailed Isotonic Transform: Untransformed Conc-mg!L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 32.400 1.0000 32.400 28.000 36.000 7.157 10 32.500 1.0000 600 32.600 1.0062 32.600 29.000 38.000 7.542 10 -0.169 2.287 2.711 32.500 1.0000 800 32.300 0.9969 32.300 30.000 36.000 6.535 10 0.084 2.287 2.711 32.300 0.99311 1000 30.800 0.9506 30.800 29.000 32000 4.275 10 1.350 2.287 2711 30.800 0.9477 *1200 17.100 0.5278 17.100 11.000 23.000 24.726 10 12.905 2.287 2.711 17.100 0.5262 *1400 6.700 0.2068 6.700 3.000 10.000 38.569 10 21.677 2.287 2.711 6.700 0.2062 Auxiliary Tests Statistic Critical Skew K11rl Ko1mogorov D Test indicates normal distribution (p > 0.01) 0.7737003 1.035 -0.03508 J 8 -0.1738142 Bartlett's Test indicates equal variances (p = 0.03) 12.0027618 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 1000 1200 J 095.44512 2. 71098343 0.08367233 1192.29667 7.02777778 9.9E-32 5. 54 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point m~L SD 95%CL Skew
!COS 990 77.815583 773.3125 1014.27169 -2.4281 1CIO 1022.62774 1!.2990174 1002.70019 1038.77377 -2.1436 !C15 1046.35036 9.57591142 1027.49595 1064.20095 0.0648 TC20 1070.07299 10.5377883 1048.11784 1091.92162 0.3416 JC25 1093.79562 11.9318091 1072.19759 1121.37833 0.5359 JC40 1164.9635 17.2015679 1138.07325 1205.85798 0.6225 IC50 1216.34615 20.7905444 1181.83123 1255.24757 0.1597 /0-07-08
Page .5 of 6 Species: Ceriodaphnia dubia Cd~aCLCR #: 60 CONTROL 600 mg NaCl/L 800 mg NaCl/L 1000 mg NaCI/L 1200 mg NaCl!L 1400 mg NaCl!L STOCK Page 95 of96
Page 6 of6 Cd~aCLCR 'f: &() Species: Ceriodaphnia duhia CONTROL 600 mg NaCI/L 800 mg NaCI/L 1000 mg NaCI/L 1200 mg NaCIIL 1400 mg NaCI/L STOCK I Page 96 of 96
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Na~-~A-SEQUOYA~UCLEA~LANT ___ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L_£.~BOX2000 - - - - - - - - - - - - TN0026450 103 G F- FINAL ---~TEROFFICESB-~---------- _ _ _ 20DD_L- DAISY_ _IN 37381._ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Facility_ JYA -_§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!]_ .JjAMIL TO..ti_COUNT:L_ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT From YEAR I MO DAY I DAY NO DISCHARGE D ... ATTN: Stephanie A. Howard 08 10 01 To 31 NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION I NO. EX 0 0 0 DUIT OR THRU PLANT 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary . properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is . to the best of my knowledge and belief, true, 423 843-6700 08 11 13 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information. including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR P.RINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Add res.§_ _E Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 107 G F- FINAL - _ _ _§.ODD_X,_- DAISY_ _JN 37381._ _ _ _ _ _ _ _ METAL CLEANING WASTE POND FaciliN_~A-SEQUO~~NUCLEARP~N~----- Locatio.n._ .J::!AMIL TO.!:!_COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard YEAR From I 08 , . - 1 -- I DAY 31 NO DISCHARGE I XX I *** NOTE: Read instructions before completinq this form. PARAMETER NO. IFREQ_UENCYI SAMPLE EX OF TYPE ANALYSIS 0 0 0 0 FFLUENT GROSS VALUE RON, TOTAL (AS FE) 0 0 CONDUIT OR THRU ENT PLANT 1 0 0 EFFLUENT GROSS VALUE Jh.n~t~ ... ~ a.~~ NAME!TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or I)""T ""'
persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Site Vice President Information, the information submitted is , to the best of my knowledge and belief, true, 423 I 843-6700 08 11 13 accurate, and complete. I am aware that there are significant penalties tor submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fme and Imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED
---- ------- ---- ------- - - - - - - - * * * -~()t)E I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres,L __E.~BOX.l.QQ.Q_ _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 110 G F- FINAL - _ _ _§ODD..Y_- DAISY_ _IN 3738i_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facility_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ locatio.!J..._ _!:!AMIL TO..!i_COUNTY __________ _ EFFLUENT YEAR DAY NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard From 08 31 NOTE: Read instructions before completin~ this form. PARAMETER QUALITY OR CONCENTRATION j NO. EX MINIMUM AVERAGE MAXIMUM PERATURE, WATER DEG. ******** ******** NTIGRADE z 0 0 0 0 CONDUIT OR THRU ITI:>J:ATUENT PLANT 50050 1 0 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL 0 0 NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my Timothy P. Cleary direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate lhe information submitted. Based on my inquiry of the person or (.l...._d TELEPHONE DATE persons who manage lhe system, or those persons directly responsible for gathenng the Principal Environmental Engineer information, the Information submitted is , lo lhe best of my knowledge and belief, true, 423 843-6700 08 11 13 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the poSSibility of fine and imprisonment for knowing violations_ OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _f.~BOX.1.QOO _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 110 T F- FINAL ---~TEROFFICESB-~---------- - _ _ ....§.ODD...Y._- DAISY_ __JN 3738!._ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facility_ JYA -.§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l._ .J::!AMILTOJ:i.COUNTY__________ _ MONITORING PERIOD EFFLUENT YEAR I MO I DAY I I YEAR I MO I DAY ATTN: Stephanie A. Howard From 08 I 10 I 01 I To I 08 I 10 I 31 NO DISCHARGE IXX I *** NOTE: Read instructions before completinq this form. PARAMETER STATRE 7DAY CHR RIODAPHNIA 0 0 0 0 ENT GROSS VALUE NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE Timothy P. Cleary direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or (} persons who manage the system. or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 843-6700 08 11 13 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~~-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR01) Addres_L _E.~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 116 G F- FINAL ---~TEROFFICESB-~---------- - _ _ _§ODDY- DAISY_ _JN 3738L _ _ _ _ _ _ _ BACKWASH Facility_ ...IYA -_§EQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!}_ ..J::!AMIL TOl!.._COUNTY.._ _ _ _ _ _ _ _ _ _ _ EFFLUENT YEAR DAY NO DISCHARGE [J *** ATIN: Stephanie A. Howard From I 08 I . - I -
I 31 NOTE: Read instructions before completinq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQ EX OF ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS DEBRIS, FLOATING (SEVERITY) ******** ******** ******** ******** 0 0 9A 01345 0 0 EFFLUENT GROSS VALUE LAND GREASE VISUAL 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE Timothy P. Cleary direction or supervision in accordance wtth a system designed to assure that qualtfied personnel properly gather and evaluate the information submitted. Based on my tnquiry of the person or persons who manage the system. or those persons directly responsible for gathering the G ClA--d Principal Environmental Engineer tnformation, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 08 11 13 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the posstbility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Prev1ous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facititv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L_E.Q,_BOX_1900 - - - - - - - - - - - - ---~TEROFFICESB-~---------- TN0026450 117 G F- FINAL _ _ _ __§.ODDY- DAISY_ _JN 3738L _ _ _ _ _ _ _ BACKWASH Facility_ JYA -_§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio_ll_ J:!AMIL TO!i_COUNTY__________ _ EFFLUENT ATTN: Stephanie A. Howard From YEAR L 08 .- - - DAY 31 NO DISCHARGE LJ *** NOTE: Read instructions before completinQ this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IUt:Ct'(t,::,, FLOATING (SEVERITY) ******** ******** .. ******** ******** 0 9A 0 1 I 31 VISUAL NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my Timothy P. Cleary direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the a.-c-J TELEPHONE DATE Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 08 11 13 Site Vice President accurate, and complete_ I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and impnsonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Na~-~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L_f~BOX_1900 - - - - - - - - - - - - ---~TEROFFICESB*~---------- TN0026450 118 G F- FINAL _ _ _ ..§.ODD..Y...- DAISY_ _IN 3738L _ _ _ _ _ _ _ WASTEWATER & STORM WATER Facility_ .:JYA -..§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.Q_ .J::jAMIL TO.!!.COUNTY __________ _ EFFLUENT YEAR DAY NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard From I 08 I .- I -
I 31 NOTE: Read instructions before completinq this form.
PARAMETER QUALITY OR CONCENTRATION I NO. IFREQ SAMPLE EX OF TYPE ANALYSIS MAXIMUM NITS N, DISSOLVED (DO) ******** 00300 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 0 0 FLUENT GROSS VALUE OLIOS, SETTLEABLE 0 0 NDUIT OR THRU PLANT 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance w1th a system des1gned to assure that qualif1ed personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Site Vice President information, the mtormat1on submitted is , to the best of my knowledge and belief, true, 423 843-6700 I 08 11 13 accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE informatiOn, Including the poss1bllity of fine and imprisonment for knowing VIOlations OFFICER OR AUTHORIZED AGENT AREA MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320*1 (REV 3/99) Previous editions may be used Page 1 of 1
S58 081210 800- NPDES CORRESPONDENCE December 10, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR NOVEMBER 2008 Enclosed is the November 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sin?erely.: ~~ _ *. I) , 0 \tl_Qft,UO-A
. d Stephanie A. Howard Principal Environmentaf Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN J. D. Smith, OPS 4A-SQN T. P. Cleary, OPS 4A-SQN G. R. Signer, WT 6A-K D. E. Pittman, BR 4T-C EDMS, WT CA-K (Enclosure) DMR0811.doc
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 December 10, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1 6 h Floor, l & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR NOVEMBER 2008 Enclosed is the November 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments.
lt<_Jc~'- 0 .~lcr"UCM d Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure)

Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Add res.§_ _E.~BOX..£900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 101 G F- FINAL - _ _ ....§.ODD...Y...- DAISY_ _IN 37381_ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facility_ JYA -_§E.QUOYJlli_NUCLEAR PLANT_ _ _ _ _ _ Locatio.!}_ .J:!AMIL TO.N._COUNTY__________ _ MONITORING PERIOD EFFLUENT From YEAR DAY NO DISCHARGE D ... ATTN: Stephanie A Howard 08 To 30 NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQ EX OF ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM
, WATER DEG. ******** ******** ******** ******** 19.6 04 0 0
0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFIC~~--t Certify under penalty of law that th1s document and all attachments were prepared under my !~~ d~rectlon or superv1sion 1n accordance w1th a system des1gned to assure that qual1f1ed personnel I 1
. *fl.-LL 0 ~ }1 (i:.A:_ Q, TELEPHONE DATE I
Timothy P. Cleary !properly gather and evaluate the Information submitted Based on my 1nqU1ry of the person or ' 1 i persons who manage the system, or those persons d~rectly responsible for gathenng the Principal Environmental Engineer I !I 08 I I
. . . information, the mformat1on submitted IS , to the best of my knowledge and belief, true, 1 423 I 843-6700 12 1 10 S1te Vtce President accurate, and complete. 1am aware that there are sign1ficant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE i i information, mcluding the possibility of fine and imprisonment for know1ng v1olations.
TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT I AREA CODE I NUMBER YEAR I MO I DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3199) PreVious editions may be used Page 1 of 2
DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analy~is Date/Time Analyst Method 11/12/2008 @ 1212 0.32 mg/1 11/14/2008@ 1340 CLS TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 11/12/2008@ 1210 <0.10 mg/1 11/14/2008@ 1350 CLS TN EPA 8015
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres.L _E.~BOX...£900 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 G F- FINAL ---~TEROFFICESB-~---------- - _ _ __§ODD..Y.: DAISY _ _.IN 3738£_ _ _ _ _ _ _ _ DIFFUSER DISCHARGE fscilitv_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ LocatiO!)_ .J::!AMILTOli_COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard YEAR From I 08 I *
I -

DAY 30
*** NO DISCHARGE D ...
NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. !FREQUENCY! SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS RINE, TOTAL RESIDUAL ******** ******** ******** 0.021 0.043 GRAB 19 0 0 E -C, RATE OF 0 0 GROSS VALUE
~l.LLb'~.-l_c_ U.cUV\.L)Ct-t'--'
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penally of law lhallh1s document and all attachments were prepared under my TELEPHONE DATE dJrect1on or supervision 1n accordance with a system des1gned to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the Information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information subm1tted is . to the best of my knowledge and belief, true, 423 843-6700 08 12 10 Site Vice Presid~nt accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occurred: 1. H-150M (max. calc. cone. was 0.0411 mg/L--Iimit 0.050mg/L) 2. H-150M (low detection level analytical method was <0.020mg/L--Iimit 0.050mg/L) 3. MSW 101 (low detection level analytical method was 0.030mg/L--Iimit 0.20mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ Form Approved MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres,L _E.~BOX.1fl00 ___________ _ TN0026450 101 T F- FINAL -~-~TEROFFICESB*~---------- - _ _ _§.ODD..Y..- DAISY_ _JN 3illL _ _ _ _ _ _ _ BIOMONITORING FOR OUTFALL 101 Facility_ JYA -~QUOYAH NUCLEAR PLANT_ _ _ _ _ _ locatio.!)_ ..J:!AMILTOJi.COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard From I 08 YEAR I .. I - - I DAY 30 NO DISCHARGE D ... NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. EX IFREQUENCY OF I SAMPLE TYPE AVERAGE 7DAY CHR 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direction or supervision 1n accordance with a system des1gned to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the informat1on submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true. 843-6700 08 12 10 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information. including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in November 2008. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Add res.§_ .J: ~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 103 G F- FINAL - _ _ _§.ODDY- DAISY_ _IN 3?2§L _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Facility_ JYA - SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.n_ .J:!AMILTOl:LCOUNTY __________ _ MUNI JKING PERIOD EFFLUENT I DAY I I YEAR I MO I ATIN: Stephanie A. Howard From YEAR I 08 I 11 MO I 01 I To I 08 I 11 I DAY 30
*** NO DISCHARGE D ***
NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION \ NO. \FRE~I.JENCY\ SAMPLE TYPE AVERAGE
******** GRAB 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 0 0 LAND GREASE 00556 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 1 0 0 LUENT GROSS VALUE ~Wt-~0~
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law thai this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance wilh a system designed to assure thai qualified personnel Timothy P. Cleary properly gather and evaluate the informalion submitted. Based on my inquiry of the person or persons who manage lhe system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 08 12 10 Site Vice President accurate. and complete. I am aware that there are significant p~naltles for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED mformatlon, including the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMS No. 2040-0004 (SUBR 01) Addres,L _E ~BOX 20QQ.. _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 107 G F- FINAL ---~TEROFFICESB-~---------- - _ _ ~ODDY- DAISY_ _IN 3738L _ _ _ _ _ _ _ METAL CLEANING WASTE POND Facility_ JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio..!J_ _!:!AMILTOJi.COUNTY __________ _ EFFLUENT YEAR NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard From I os I -- I -- I PARAMETER QUANTITY OR LOADING AVERAGE PH ******** 0 0 1sn1 ms, TOTAL SUSPENDED 0 0 0 0 EFFLUENT GROSS VALUE PHOSPHORUS, TOTAL (AS P) 00665 0 0 EFFLUENT GROSS VALUE TOTAL (AS CU) 0 0 FFLUENT GROSS VALUE RON, TOTAL (AS FE) 0 0 CONDUIT OR THRU ENT PLANT 0 0 EFFLUENT GROSS VALUE NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 843-6700 08 12 10 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRI OMB No. 2040-0004 (SUBR 01) Addres.L_E.SL_BOX_1900 - - - - - - - - - - - - TN0026450 110 G F- FINAL ---~TEROFFICESB-~---------- ---~DDY-DAISY_~N3738L _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER I RECYCLED COOLING WATER Facility_ JYA -.§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!!_ .J::!AMILTOJi_COUNTY __________ _ MONITORING PERIOD EFFLUENT YEAR I YEAR I MO I DAY NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard From 08 To 08 11 30 NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION I NO. IFREQUENCY I SAMPLE TYPE MINIMUM AVERAGE MAXIMUM UNITS
******** ******** 04 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 0 0 EFFLUENT GROSS VALUE LAND GREASE 0 0 DUITORTHRU ITCCATIUICIIJT PLANT 0 0 ~w__a~~
NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is . to the best of my knowledge and belief, true, 423 I 843-6700 08 12 10 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRI OMB No. 2040-0004 (SUBR 01) Addres_L ..f..~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 F- FINAL ---~TEROFFICESB-~---------- ---~DDY-MIS~~N373BL ______ _ RECYCLED COOLING WATER Facilitv_ _.IVA-~QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio!!_ .J::!AMILTOl:!.COUNTY__________ _ EFFLUENT YEAR NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard From I 08 I -- I - - I NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION I NO. IFREQUENCY I SAMPLE EX OF TYPE ANALYSIS STATRE 7DAY CHR ODAPHNIA 0 0 M~LhiJer~o-._J NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief. true, 423 I 843-6700 08 12 10 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Add res§_ _E.~BOX.lQQQ_ _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 116 G F- FINAL ---~D~DAIS~~N3738L ______ _ PERMIT NUMBER DISCHARGE NUMBER BACKWASH Facility_ .JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l.._ _!:!AMILTOli.COUNTY __________ _ MONITORING PERIOD EFFLUENT I I DAY I I YEAR I MO I ATTN: Stephanie A. Howard From YEAR 08 I MO 11 I 01 J To I 08 I 11 DAY J 30 NO DISCHARGE D *** NOTE: Read instructions before complelinQ this form. PARAMETER NO. IFREQUENCYI SAMPLE EX OF TYPE ANALYSIS UNITS MINIMUM BRIS, FLOATING (SEVERITY) ******** 0 1 I 30 VISUAL 1 0 0 FFLUENT GROSS VALUE LAND GREASE VISUAL 0 0 NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my ~ *~ (A~~ TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel . *\)....L_ . \ . Timothy P. Cleary properly gather and evaluate the informalion submitted. Based on my InqUiry of the person or persons who manage the system. or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is . to the best of my knowledge and belief. true, 423 843-6700 08 12 10 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approv~c1 Na~-~A-SEQUOYA~UCLEARP~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Addres,L __E.Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB*~---------- TN0026450 117 G F- FINAL ---~ODDY-DAIS~~N3738L ______ _ PERMIT NUMBER DISCHARGE NUMBER BACKWASH Facility_ JYA
SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _
Locatio.!!_ .J::!AMILTOJi.COUNTY __________ _ MONITORING PERIOD EFFLUENT YEAR I MO I DAY I I YEAR I MO DAY NO DISCHARGE D ... ATTN: Stephanie A. Howard From I 08 11 01 To 08 11 30 NOTE: Read instructions before completinq this form. PARAMETER NO. !FREQUENCY! SAMPLE EX OF TYPE ANALYSIS IDEBRIS, FLOATING (SEVERITY) 0 1 I 30 VISUAL 0 0 ENT GROSS VALUE 0 0 ENT GROSS VALUE
~u~_J._[t_~~
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 08 12 10 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED information, including the possrbility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE lI NUMBER I YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARP~NT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _e.~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 118 G F- FINAL _ _ _ 20DDY-DAISY_~N373BL _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER Facility_ JYA-_g:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l_ .J::!AMILTOli_COUNTy_ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT YEAR DAY From NO DISCHARGE I XX I *** ATTN: Stephanie A. Howard 08 To 30 NOTE Read instructions before completinq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION J NO. /FREQUENCY SAMPLE EX ' OF TYPE ANALYSIS AVERAGE MAXIMUM MINIMUM (DO) ******** ******** ******** ******** 19 0 0 GROSS VALUE 0 0 EFFLUENT GROSS VALUE
/J~t\[U'\.,i.:_e_ (A~I;J({A.d NAME!TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is . to the best of my knowledge and belief, true, 423 843-6700 08 12 10 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE 1 NUMBER YEAR MO I
DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
S58 090217 800 - NPDES Correspondence February 17, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT (SON)- NPDES PERMIT NO. TN0026450-CORRECTION TO DISCHARGE MONITORING REPORT FOR DECEMBER 2008 During the review of the Biocide/Corrosion Treatment Plan (B/CTP) it was noted that three total residual chloride analyses had been omitted from the December 2008 Discharge Monitoring Report (DMR). Please see the attached corrected DMR page. It should be noted that the chlorine, total residual maximum value remained the same at 0.032mg/L and the chlorine, total residual average value increased from 0.017mg/L to 0.018 mg/L. Please contact me at (423) 843-6700 or by email at sahoward@tva.gov if you have any questions or need additional information. Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah .Nuclear Plant
Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control ATTN: Document Control Desk State Office Building, Suite 550 Washington, D.C. 20555 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN B. A. Wetzel, OPS 4A-SQN T. P. Cleary, OPS 4A-SQN G. R. Signer, WT 6A-K D. E. Pittman, BR 4T-C EDMS, WT CA-K (Enclosure)
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- CORRECTION TO DISCHARGE MONITORING REPORT FOR DECEMBER 2008 ORGANIZATION: Chemistry and Environmental DOCUMENT PREPARED BY: Ann Hurt oCA.. DATE: 02/13/2009 ~-N*o 11 Boo EDMS TRACKING NO.: S58 090213 802 NPDES Correspondence CONCURRENCES Name R c Signature - Comment Date v N Ann Hurt X zlr~\ zoo4 Stephanie A Howard X 2.. \ r~\oq Alan K Barringer X v). Debra J Bodine X Beth A. Wetzel X ;2 Chris R Church X z Timothy P Cleary X I ~FEE;o"t Stephanie A. Howard (for signature) X z}ICl}ocy INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 February 17, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEOUOYAH NUCLEAR PLANT (SON)- NPDES PERMIT NO. TN0026450-CORRECTION TO DISCHARGE MONITORING REPORT FOR DECEMBER 2008 During the review of the Biocide/Corrosion Treatment Plan (B/CTP) it was noted that three total residual chloride analyses had been omitted from the December 2008 Discharge Monitoring Report (DMR). Please see the attached corrected DMR page. It should be noted that the chlorine, total residual maximum value remained the same at 0.032mg/L and the chlorine, total residual average value increased from 0.017mg/L to 0.018 mg/L. Please contact me at (423) 843-6700 or by email at sahoward@tva.gov if you have any questions or need additional information. Sincerely, MeutU_ o.~o.-ud Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant
Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control ATTN: Document Control Desk State Office Building, Suite 550 Washington, D.C. 20555 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ MAJOR Form Approved Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L ....E.Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 G F- FINAL ---~TEROFFICESB-~---------- ---~D~DAIS~~N3738L ______ _ DIFFUSER DISCHARGE PERMIT NUMBER DISCHARGE NUMBER Facility_ .JYA -_g:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l.._ .J:!AMILTOl::!..COUNTY __________ _ MONITORING PERIOD EFFLUENT ATIN: Stephanie A Howard From YEAR I 08 MO I DAY I I YEAR I MO I 12 I 01 I To I 08 I 12 I 31 I DAY NO DISCHARGE U *** NOTE: Read instructions before completinQ this form. PARAMETER NO. IFREQUENCYI SAMPLE EX OF TYPE ANALYSIS HLORINE, TOTAL RESIDUAL 0 21 I 31 GRAB 0 0 CALCTD CALC TO 0 0 EFFLUENT GROSS VALUE I
~0.~1cv.d NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the Information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the Principal Environmental Engineer information. the information submitted is, to the best of my knowledge and belief. true, 423 843-6700 09 02 11 Site Vice President accurate, and complete. I am aware that there are srgnificant penalties for submitting false information, includmg the possibility of fine and imprisonment for knowing violations SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY CODE I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
The following injections occurred: 1. Biodetergent 73551 (max. calc. cone. was 0.017mg/L--Iimit 2.0mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2
Address Information Ship to: Ship from: Mr. Patrick Cromer Ruth Ann Hurt TDEC- Div. ofWater TVA Pollution 6th Floor, L & C Annex SEQUOYAH NUCLEAR PLANT 401 Church Street Nashville, TN SODDY DAISY, TN 372431534 37379 us us 4238436700 4238436714 Shipping Information Tracking number: 797348126247 Ship date: 02/19/2009 Estimated shipping charges: 40.35 Package Information Service type: First Overnight Package type: FedEx Envelope Number of packages: 1 Total weight: O.lLBS Declared value: O.OOUSD Special Services: Pickup/Drop-off: Give to scheduled courier at my location Billing Information Bill transportation to: Sender Your reference: P.O. no.: Invoice no.: Department no.: Please Note Fed Ex will not be responsible for any claim in excess of $100 per package, whether the result of loss, damage, delay, non*delivery, misdelivery, or misinformation, unless you declare a higher value, pay an additional charge, document your actual loss and file a timely claim. Limitations found in the current Fed Ex Service Guide apply. Your right to recoverfrom FedEx for any loss, including intrinsic value of the package, loss of sales, income interest, profrt, attorney's fees, costs, and other forms of damage whether direct, incidental, consequential, or speical is limited to the greater of $100 or the authorized declared value. Recovery cannot exceed actual documented loss. Maximum for items of extraordinary value is $500, e.g., jewelry, precious metals, negotiable instruments and other items lised in our Service Guide. Written claims must be filed within strict time limits; Consult the applicable FedEx Service Guide for details. The estimated shipping charge may be different than the actual charges for your shipment. Differences may occur based on actual weight, dimensions, and other factors. Consult the applicable f:~Q..E;;--s_§~[YJf:.~_G_~jQ_~_ or the F edEx Rate Sheets for details on how shipping charges are calculated. https://www.fedex.com/shippinglhtml/en/PrintiFrame.html 02/18/2009
Address Information Ship to: Ship from: Mr. Mike Kelley Ruth Ann Hurt Chattanooga EAC - Div. of TVA Water State Office Building, Suite SEQUOYAH NUCLEAR 550 PLANT 540 McCallie Avenue Chattanooga, TN SODDY DAISY, TN 374022013 37379 us us 423-843-6700 4238436714 Shipping Information Tracking number: 796351781523 Ship date: 02119/2009 Estimated shipping charges: 4.52 Package Information Service type: Priority Overnight Package type: FedEx Envelope Number of packages: 1 Total weight: 0.1LBS Declared value: O.OOUSD Special Services: Pickup/Drop-off: Give to scheduled courier at my location Billing Information Bill transportation to: Sender Your reference: P.O. no.: Invoice no.: Department no.: Please Note FedEx will not be responsible for any claim in excess of $100 per package, whether the result of loss. damage, delay, non-delivery, misdelivery, or misinformation, unless you declare a higher value, pay an additional charge, document your actual loss and file a timely claim. Limitations found in the current F edEx Service Guide apply. Your right to recover from FedEx for any loss. including intrinsic value of the package, toss of sales, income interest, profit. attorney's fees, costs, and other forms of damage whether direct. incidental, consequential, or speical is limited to the greater of $100 or the authorized declared value. Recovery cannot exceed actual documented loss. Maximum for items of extraordinary value is $500, e.g., jewelry, precious metals, negotiable instruments and other rtems lised in our Service Guide. Written claims must be filed within strict time limits; Consult the applicable FedEx Service Guide for detailS. The estimated shipping charge may be different than the actual charges for your shipment. Differences may occur based on actual weight, dimensions, and other factors. Consult the applicable 1:~9-~'£.§~-~Yi~~-<l'-:t!Q~ or the Fed Ex Rate Sheets for details on how shipping charges are calculated. https://www.fedex.com/shipping/html/en/PrintiFrame.html 02/18/2009
Address Information Ship to: Ship from: To whom it may concern: Ruth Ann Hurt Nuclear Regulatory TVA Commission ATTN: Document Control SEQUOYAH NUCLEAR Desk PLANT Washington, DC SODDY DAISY, TN 20555 37379 us us 423-843-6700 4238436714 Shipping Information Tracking number: 797348133411 Ship date: 02/19/2009 Estimated shipping charges: 4.52 Package Information Service type: Priority Overnight Package type: FedEx Envelope Number of packages: 1 Total weight: 0.1LBS Declared value: O.OOUSD Special Services: Pickup/Drop-off: Give to scheduled courier at my location Billing Information Bill transportation to: Sender Your reference: P.O. no.: Invoice no.: Department no.: Thank you for shipping online with Fedex ShipManager at fedex.com. Please Note Fed Ex will not be responsible for any claim in excess of $100 per package, whether the result of loss, damage, delay, non-delivery, misdelivery, or misinfotmation, unless you declare a higher value, pay an additional charge, document your actual loss and file a timely claim. Limitations found in the current F edEx Service Guide apply. Your right to recover from FedEx for any loss, including intrinsic value of the package, loss of sales, income interest. profrt, attorney's fees, casts, and other forms of damage whether direct, incidental, consequential, or speical-is limited to the greater of $100 or the authorized declared value. Recovery cannot exceed actual documented loss. Maximum for items of extraordinary value is $500, e.g., jewelry, precious metals, negotiable instruments and other items lised in our Service Guide. Written claims must be filed within strict time limits; Consult the applicable FedEx Service Guide for details. The estimated shipping charge may be different than the actual charges for your shipment. Differences may occur based on actual weight, dimensions, and other factors. Consult the applicable f.~Q..f;;"'i_§~r.Yi.~_Q.oJjQ~ or the F edEx Rate Sheets for details on how shipping charges are calculated. https://www.fedex.com/shipping/html/en/PrintiFrame.html 02/18/2009
S58 090112 800- NPDES CORRESPONDENCE January 12, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR DECEMBER 2008 Enclosed is the December 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, I /~1Ci.Jl.AL 0 ~0CU- d Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant AH Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 cc: D. J. Bodine, POB 2A-SQN A. A. Ray, WT 7C-K C. R. Church, POB 2B-SQN J.D. Smith, OPS 4A-SQN T. P. Cleary, OPS 4A-SQN G. R. Signer, WT 6A-K D. E. Pittman, BR 4T-C EDMS, WT CA-K (Enclosure) DMR0812.doc
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384*2000 January 12, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR DECEMBER 2008 Enclosed is the December 2008 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, )~t~l/A ~cued Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Add res§_ ..£.~BOX..£900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 101 G F- FINAL _ _ _ 20D~DAISY_~N3738L _ _ _ _ _ _ _ DIFFUSER DISCHARGE Facilitv_ _IVA -..§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!}_ _.!jAMIL TO..ti.COUNTY __________ _ EFFLUENT ATTN Stephanie A. Howard YEAR From I 08 I *- I -
I DAY 31 NO DISCHARGE LJ ***
NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION i NO. [FREQUENCYj-SAMPLE I EX I OF ANALYSIS I TYPE I AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS
******** ******** ******** ******** 13.3 04 0 31 I 31 I MODELD . DIFF. BETWEEN SAMP. &
UPSTRM DEG.C 1 w 0 EFFLUENT GROSS VALUE PH 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 0 0 0 0 GROSS VALUE
, IN CONDUIT OR THRU ENT PLANT 1 0 0 EFFLUENT GROSS VALUE I NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER ffi~~ ClcL!vvuU-kd I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel '
Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environment<;~! Engineer Site Vice President Information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete I am aware that there are s1gnif1cant penalties for submittmg false 423 843-6700 j 09 II 01 II 12 SIGNATURE OF PRINCIPAL EXECUTIVE I
\YEAR I 1nformat1on, 1ncluding the possibility of fine and imprisonment for knowing vtolations OFFICER OR AUTHORIZED AGENT AREA ! NUMBER MO I DAY TYPED OR PRINTED I I CODE ! ' i COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attacHments here)
No closed mode operation. The following information is included in an attachment: 1. CCW data 2. veliger monitoring data EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 2
DMR Attachment CCW Data CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/1 0/2008 @ 1220 0.17mg/1 12/12/2008@ 0956 KLM TN EPA 8015 CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/10/2008@ 1215 <0.10 mg/1 12/12/2008 @ 1006 KLM TN EPA 8015
Mean# of NOTES:% Mean# of Water Temp. Water Temp. LOCATION SUB Sample Date % Settlers ("C) Sample Date Asiatic Gravid Asiatic COLLECTED BY ZM/m3 ("C) LOCATION Clams/m3 Clam 11/21/2008 0 0 12 11/16/2008 230 12 In plant RCW Dick Adcock 11/24/2008 31 0 11 11/24/2008 138 11 In plant RCW Dick Adcock 12/4/2008 0 0 10 12/04/2008 15 10 lnplant RCW Dick Adcock 12/8/2008 0 0 9 12/08/2008 0 9 In plant RCW Dick Adcock 12/15/2008 0 0 9 12/15/2008 0 9 lnplant RCW Dick Adcock 12/22/2008 0 0 9 12/22/2008 0 9 In plant RCW Dick Adcock 12/29/2008 0 0 9 12/29/2008 0 9 In plant RCW Dick Adcock 01/09/2008 0 0 8 01/09/2009 0 8 In plant RCW Dick Adcock
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _E 9.c._BOX..1_900 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 101 G F- FINAL ---~TEROFFICESB-~~--------- - _ _ _§ODD..Y_- DAISY_ _Jti]7381.._ _ _ _ _ _ _ _ DIFFUSER DISCHARGE Facility_ .JYA -_§l:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio!l_ .J:!AMIL TO.!i_COUNTL_ _ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN: Stephanie A. Howard From LOS YEAR NO DISCHARGE CJ *** NOTE: Read instructions before completinq this form. PARAMETER QUALITY OR CONCENTRATION I NO. IFREQUENCY I SAMPLE EX OF TYPE ANALYSIS MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS LORINE, TOTAL RESIDUAL ******** ******** 0.017 0.032 0 18 I 31 GRAB 19 URE- C, RATE OF 0 0 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penalty of law that this docu. ment and all attachments w.ere prepared under my
.:.::.=:::..:__=_:__:_.:_:__:_.:.::_:::.__:_:_:__:_..=__:__:_:=~direct*on or supervision in accordance w*th a system des1gned to assure that qualified personnel r... * .. * }fr h.G(j' 1'\ rd .* , " UA (IJ_(/).,<...)Cu .d TELEPHONE DATE Timothy p. Cleary properly gather and evaluate the 1nformat1on submitted. Based on my Inquiry of the person or u.c'>j) '-"'-"- '\'
personswho manage the system, or those persons d~rectly responsible for gathenng the Principal Environmental Engineer
. . . information, the 1nformat1on subm11ted IS , to the best of my knowledge and bel1ef. true, 8 4 3
6700 09 01 12 S1te V1ce President !accurate, and complete 1 am aware that there are significant pena111es for submitt1ng false SIGNATURE OF PRINCIPAL EXECUTIVE
_________ ___jlnformalion, 1nclud1ng the poss1bd1ty of f1ne and 1mpnsonment for know1ng VIOlations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED I 1
---------------- ~~~~~----------
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occurred: 1. Biodetergent 73551 (max. calc. cone. was 0.017mg/L--Iimit 2.0mg/L) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 2 of 2
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Addres_L .£.~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-m _ _ _ _ _ _ _ _ _ _ TN0026450 101 Q F- FINAL _ _ _ ~ODD.Y_- DAISY _ _JN 3738L _ _ _ _ _ _ _ DIFFUSER DISCHARGE Facility_ .:JYA
SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _
Locatio.!l_ .J::!AMILTOl:!_COUNT:L_ _ _ _ _ _ _ _ _ _ _ EFFLUENT From , __ , . _ NO DISCHARGE II ATTN: Stephanie A Howard 1 NOTE Read instructions before completinq this form. PARAMETER QUALITY OR CONCENTRATION MAXIMUM MINIMUM MAXIMUM UNITS BORON, TOTAL ******** <0.20 19 1022 1 0 0 EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law thallhis document and all attachments were prepared under my L.~ui..c~ cu.. d TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons d~rectly responsible for gathenng the Principal Environmental Engineer information, the information submitted IS to the best of my knowledge and belief, true, 423 843-6700 09 01 Site Vice President Iaccurate. and complete I am aware that there are sigmficant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE 1nformat1on. 1nclud1ng the possibility of f1ne and 1mpnsonment for know1ng VIOlations. OFFICER OR AUTHORIZED AGENT I AREA YEAR MO TYPED OR PRINTED __j_ ___ ___ ____l__C_Q_QE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Boron was sampled on 10/01/2008@ 1120. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~~~EQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Add res.§_ _E.Q;_BOX_1.900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- [ TN0026450 I 101 T F- FINAL - _ _ _§_ODD_1_- DAISY _ _]N 37381._ _ _ _ _ _ _ _ Facility _ _s:/A -_§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ [ PERMIT NUMBER _j BIOMONITORING FOR OUTFALL 101 Locatio.!}_ _t!AMIL TO.!:!._COUNTL_ _ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN: Stephanie A. Howard DAY 31 NO DISCHARGE CJ *** From 1 -- 1 *- 1 -
NOTE: Read instructions before completinq this form.
PARAMETER NO. IFREQ EX OF ANALYSIS STATRE 7DAY CHR RIODAPHNIA 1 0 0 1 0 0 EFFLUENT GROSS VALUE NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel ~Hbpha.t\AJLOJ];wUAd TELEPHONE T DATE Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathenng the Principal Environmental Engineer 423 09 01 12 1nformat1on. the information submitted is . to the best of my knowledge and belief, true. 843-6700 Site Vice President accurate, and complete I am aware that there are significant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE f - - - - - - - - - - - - - - - - - - - - - - - - j l n f o r m a t i O n , including the possib1l1ty of fine and Imprisonment for knowing violations T TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER IYEARI MO I DAYJ COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in December 2008. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No 2040-0004 (SUBR 01) Add res.§_ __f.~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~--------~- r- -* --TN002645c)- --~ 103 G F- FINAL - _ _ _§_ODDY- DAISY_ _IN 37384_ _ _ _ _ _ _ _ I PERMIT NUMBER I LOW VOL. WASTE TREATMENT POND Facility_ ..JYA -_g:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.Q_ _jjAMIL TOJ!.COUNT!::_ _ _ _ _ _ _ _ _ _ _ EFFLUENT ATIN: Stephanie A. Howard YEAR Fromj 08 I *- , -* NO DISCHARGE CJ *** NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUENCY I SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS
******** ******** 7.2 ******** 8.3 0 14 I 31 GRAB 12 0 0 1;,suuu;,s. TOTAL SUSPENDED 0 0 DUITORTHRU PLANT 0 0 I
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P Cleary Site Vice President 1 Cert1fy under penalty of law that th1s document and all attachments were prepared under my d~reclion or superv1s1on 1n accordance w1th a system des1gned to assure that qualified personnel persons who ~* ""'""""'
""""manage the system '"'"m'"oo '""m'""
or those persons '"" d~rectly oo m, '"'"'" responsible o<"" ""~" for gathenng the m 1nformat1on, the 1nformat1on subm1tted IS , to the best of my knowledge and belief, true, accurate and complete I am aware that there are Slgn1f1cant penalt1es for submitting false 1nformat1on, 1nclud1ng the poss1b1hty of f1ne and 1mpnsonment for know1ng VIOlatiOns F I 1
J~W\.A...(.._
~
(/{ ,~
\C<..--L Prtncipal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT d
423 AREA TELEPHONE lI 843-6700 NUMBER 09 YEAR DATE 01 MO 12 DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR! OMB No. 2040-0004 (SUBR 01) Addres_L __f ~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 107 G F- FINAL - _ _ _§.ODD_.Y_- DAISY_ _JN 3738L _ _ _ _ _ _ _ METAL CLEANING WASTE POND Facilitv_JYA-_§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.[!_ _!:!AMIL TO.!:!_COUNTY __________ _ EFFLUENT YEAR NO DISCHARGE I XX I *** ATIN: Stephanie A. Howard From I 08 I - I -. I NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. EX IFREQOF ANALYSIS AVERAGE PH ******** 0 0 0 1042 1 0 0 FFLUENT GROSS VALUE RON, TOTAL (AS FE) 0
, IN CONDUIT OR THRU ENT PLANT 0 0 ENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that thiS document and all attachments were prepared under my direction or supervision 1n accordance w*th a system designed to assure that qualified personnel I( t (\
Cn ,\1lCt...rtA.....e..- l
..d-l* ~/'W.)o...A. .
d TELEPHONE r DATE
-1 Timothy P. Cleary properly gather and evaluate the mformat1on submitted. Based on my inquiry of the person or u:.>f-1
persons who manage the system, or those persons dlfectly respons1ble for gathering the Principal Environmental Engineer O OQ O 1nformat1on. the mformat1on subm1tted 1s , to the best of my knowledge and belief, true, 423 843-670 1 12 Site Vice President accurate, and complete. I am aware that there are significant penalties for submittmg false SIGNATURE OF PRINCIPAL EXECUTIVE f--------------------1~information, including the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED

CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location 1f Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved MAJOR Na~-~A-SEQUOYA~UCLEA~LANT ___ _ DISCHARGE MONITORING REPORT (OMR) OMB No. 2040-0004 (SUBR 01) Add res.§_ _E.~BOJ<.1.900 ___________ _ ---~TEROFFICESB*~---------- TN0026450 I 110 G i F- FINAL - _ _ _20DD..Y_- DAISY _ _IN 37381._ _ _ _ _ _ _ _ I DISCHARGE NUMBER I RECYCLED COOLING WATER Facility_ ...JYA -..§E:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatioll_ .J:!AMIL TOJi.COUNTY__________ _ EFFLUENT YEAR DAY NO DISCHARGE I XX I *** ATTN: Stephanie A Howard From I os I .- I - . I 31 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. /FREQUENCY/ SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM E, WATER DEG. ******** ******** 04 ******** ******** 04 0 0 0 0 1 0 0 FFLUENT GROSS VALUE RINE, TOTAL RESIDUAL 0 0 ~NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I' Cert1fy under penalty of law that this document and all attachments were prepared under my Timothy P. Cleary drrectron or supervrsron 1n accordance wrth a system des1gned to assure that qualified personnel
'properly gather and evaluate the 1nformat1on submitted Based on my 1nqU1ry of the person or cu.d TELEPHONE DATE persons who manage the system, or those persons drrectly responsrble for gathering the Principal Environmental Engineer 12 tnformation, the information submitted is , to the best of my knowledge and bel1ef, true, Site Vice President accurate, and complete. I am aware that there are significant penalt1es for submitttng false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violationsc OFFICER OR AUTHORIZED AGENT DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) Form Approved. MAJOR Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMR) (SUBR 01) OMB No. 2040-0004 Add res§_ ___E Q,_BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- I TN0026450
~----* --.,-{o-rl F- FINAL
- _ _ __20DDY- DAISY_ _IN 37381._ _ _ _ _ _ _ _ PERMIT NUMBER DISCHARGE NUMBER j RECYCLED COOLING WATER Facility_ .JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!!_ _!:!AMILTO.!i_COUNTY._ _ _ _ _ _ _ _ _ _ _ MONITORING PERIOD EFFLUENT ATTN: Stephanie A. Howard From Y~R I ~2 I ~A1Y I To ~~Y NO DISCHARGE [xxJ *** NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUENCY I SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS
******** ******** ******** ******** 23 ~fuu~ 0 .rilewcu d NAME!TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE directton or superviston 1n accordance wtth a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 09 01 12 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA CODE l NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATI.ON SYSTEM (NPDESJ Form Approved. MAJOR Na~-~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (OMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _.E.\1_BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~-----~---- r--moo26450-l I 116 G I F- FINAL - _ _ ~ODD..Y_- DAISY _ _..::rN 37381_ _ _ _ _ _ _ _ Facility_ JYA -_§EQUOYAH NUCLEAR PLANT_ _ _ _ _ _ I PERMIT NUMBER II DISCHARGE NUMBER I BACKWASH Locatio.[!_ _!:jAMIL TOl:!_COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard From I 08 YEAR I -- I -
I DAY 31 NO DISCHARGE D ...
NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. IFREQUENCY I SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IUI::I:H~I::>, FLOATING (SEVERITY) ******** ******** ******** ******** 1 I 31 VISUAL 0 9A 0 0 0 ENT GROSS VALUE
~btc:uL-U- 0.. ~LA--d NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE d1rection or supervision in accordance with a system designed to assure that qualif1ed personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Principal Environmental Engineer information, the information submitted is , to the best of my knowledge and belief, true, 423 843-6700 09 01 12 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED Information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. Name_~A-SEQUOYA~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Add res§_ _E.~BOX_1900 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 117 G F- FINAL ---~D~DAISY_~N3738L ______ _ BACKWASH Facility_ .JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!.!_ .J:!AMILTO~COUNTY __________ _ EFFLUENT ATTN: Stephanie A. Howard From I YEAR 08 ; -- I -- DAY 31 NO DISCHARGE D *** NOTE: Read instructions before completinQ this form. PARAMETER QUALITY OR CONCENTRATION I NO.I FREQUENCY I SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS I i
uc.cro;1;

:., FLOATING (SEVERITY) ******** ******** ******** ******** 0 0 1 I 31 VISUAL 9A 0 0 FFLUENT GROSS VALUE IL AND GREASE VISUAL 0 0
~~ftcu~ 0 .clkwo.--. cL NAMErriTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared Ulider my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Site Vice President information, the information submitted is , to the best of my knowledge and belief. true, 423 843-6700 09 01 12 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA CODE l NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. Name_~A-SEQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Addres.2_ _E.~BOX.1.QOD _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~---------- TN0026450 118 G F- FINAL ---~ODDY-DAISY_~N3738L ______ _ WASTEWATER & STORM WATER Facility_ JYA -_§!:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio!)_ _!:!AMIL TO.!i.COUNTY EFFLUENT YEAR DAY NO DISCHARGE I XX I *** ATTN: Stephanie A Howard From I 08 I -- I -- I 31 NOTE: Read instructions before completino this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. !FREQUENCY! SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM MAXIMUM ___ ---t---- UNITS
---i----------- -----~--->---- -------~--r- +---~- ******** ******** ' -j DISSOLVED (DO) ******** , IN CONDUIT OR THRU ENT PLANT 1 0 0 EFFLUENT GROSS VALUE ~~1Cut;___Q_ Q ~wco-cl NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that th1s document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is . to the best of my knowledge and belief, true, 423 843-6700 09 01 12 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false Information, mcluding the possibility of fine and imprisonment for knowing violations.
SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREAl NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1}}

ML13289A205: Difference between revisions

Latest revision as of 19:04, 25 February 2020

Contents

Text

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

SUMMARY

Contact:

Contact:

Contact:

SUMMARY

SUMMARY

SUMMARY

SUMMARY

SUMMARY

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Navigation menu

ML13289A205: Difference between revisions

Latest revision as of 19:04, 25 February 2020

Text

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

SUMMARY

Contact:

Contact:

Contact:

SUMMARY

SUMMARY

SUMMARY

SUMMARY

SUMMARY

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Dear Mr. Patrick Cromer:

Navigation menu

Search