Text

Tennessee Valley Authority (TVA) - Sequoyah Nuclear Plant (Sqn) - NPDES Permit No. TN0026450 - Discharge Monitoring Report (DMR) for May 2012
	ML12166A199
Person / Time
Site:	Sequoyah
Issue date:	06/12/2012
From:	Carlin J T; Tennessee Valley Authority
To:	Morgan C; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
References
	TN0026450
	Download: ML12166A199 (121)
	v • d • e

{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 June 12, 2012 Ms: Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA) -SEQUOYAH NUCLEAR PLANT (SQN) -NPDES PERMIT NO. TN0026450 -DISCHARGE MONITORING REPORT (DMR) FOR MAY 2012 Enclosed is the May 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. On May 24, 2012 at 15:27 Eastern Daylight Time (EDT).yisible oil sheen was identified on the east side of the essential raw cooling water (ERCW) intake building at SQN on the Tennessee River.The source of the oil sheen was caused by an oil drip pan overflow located at the C-B ERCW traveling water screen gear drive. The leak was immediately secured and the discharge was stopped. The estimated quantity of oil discharged is less than one gallon.Because the oil spill occurred in a low flow area and favorable meteorological conditions (light winds, no rain), the water was fairly stagnant and the sheen was easily contained and cleaned up utilizing absorbent booms and pads. TVA provided oral notification to Angela Young of the Chattanooga Environmental Field Office via voice message on May 24, 2012.Documentation of the incident and corrective actions are being performed in accordance with the site's Corrective Action Program.In a letter to Mr. Vojin Janjid dated December 21, 2011 SQN notified TDEC of upcoming activities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle 18 refueling outage scheduled to begin in October 2012. This project is complete and resulted in the discharge of approximately 700 gallons of water through the SQN low volume waste treatment process. The results for the pH and TSS collected during discharge have been incorporated into the DMR. The results for the TRC have been included as an attachment to the Outfall 103 reporting form.If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. A Ms. Christina Morgan Page 2 June 12, 2012 I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sitfrite President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 PERMITTEE NAMEIADDRESS (Include Facility Name/Location if Different) Name _TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000_ -__ -NTEROFFCE OPS-5N-SQN) SODDY- DAISY TN 37384 Faciiy TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)DISCHARGE MONITORING REPORT (DMR)TNZ026450i "i 101 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD PYEAR o DAyDAY From[ 12 10 01 To l 05 1 31 MAJOR Form Approved.(SUBR 01) OMB No. 2040-0004 F -* FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE Z NOTE: Read instructions before completinq this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 39.4 04 0 31/ 31 RCORDR CENTIGRADE MEASUREMENT 00010 1 0 PERMIT Req. Mon. DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT " DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE 28.3 04 0 31/31 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 PERMIT 30.5 DEG. C. CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE 1 04 0 31/ 31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 S PERMIT 3 DEG. C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1719 0331/31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. MGD CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE *0.019 0.034 19 0 18/31 GRAB MEASUREMENT 50060 1 0 " PERMIT 0.1 .0.1 MGIL FIVE PER CALCTD EFFLUENT GROSS REQUIREMENT MO AVG, DAILY MAX WEEK TEMPERATURE -C, RATE OF SAMPLE 0 62 **-*0 31/31 CALCTD CHANGE MEASUREMENT 82234 1 0 PERMIT 2 DEG * *

CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX C/HR NUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT, NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the President information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 12 06 12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SI A"RE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)No closed mode operation. Veliger monitoring data is included as an attachment. B/CTP chemical injections during this period included 1. Floguard MS6236 (max. calc. conc. was 0.032mg/L-limit 0.2mg/L) 2. Biodetergent 73551 (max. calc. conc. was 0.032mg/L--limit 2.0mg/L) 3. Spectrus CT1300 (max. calc. conc. was 0.041mg/L--limit 0.050mg/L). EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 Mean# of Water Mean# of Water NOTES: %Sample Date ZMem3 % Settlers Ter Sample Date Asiatic Temp. VC) LOCATION SUB LOCATION Gravid Asiatic COLLECTED BY (Clamslm3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 01/17/2011 01/24/2012 01/31/2012 02/07/2012 02/14/2012 02/21/2012 02/28/2012 03/06/2012 03/13/2012 03/20/2012 03/27/2012 04/03/2012 04/10/2012 04/18/2012 May 2012 0 0 0 0 0 0 0 0 0 0 0 1623 229 79 326 0 0 0 0 0 0 0 0 0 0 0 1.3 0 20 5 9 10 13 17.6 12 8.3 26.5 11.1 11.7 13 14.6 17.2 18 22 18.8 01/10/2011 01/17/2011 01/24/2012 01/31/2012 02/07/2012 02/14/2012 02/21/2012 02/28/2011 03/06/2012 03/13/2012 03/20/2012 03/27/2012 04/03/2012 04/10/2012 04/18/2012 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 10 13 17.6 12 8.3 26.5 11.1 11.7 13 14.6 17.2 18 22 18.8 RCW 1-ISV-24-1234 1-25-545 1-25-545 1-25-545 1-24-1234 1-25-545 1-ISV-24-1234 1-ISV-24-1234 1-ISV-24-1234 1-ISV-24-1234 1-ISV-24-1234 1-ISV-24-1234 1-ISV-24-1234 1-ISV-24-1234 WBE PB WDT CR BB WE CR WBE WBE WBE WBE WBE PB PB MJW No Samples Collected PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000_ -_ _INTEROFFICE OPS-5N-SQN). _SqDDY- DAISY TN 37384 Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY___ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)DISCHARGE MONITORING REPORT (DMR)TN0026450O 1 101 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD EARM DEAR 0 DAY From 12 05 1 I To 12 05 31 MAJOR Form Approved.(SUBR 01) OMB No. 2040-0004 F -FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT NO DISCHARGE Z NOTE: Read instructions before completinq this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE >100.0 23 0 1/180 COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1 0 PERMIT 43.2 PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MINIMUM __*_"_ANNUAL , _.IC25 STATRE 7DAY CHR SAMPLE >100.0 23 0 1/180 COMPOS PIMEPHALES MEASUREMENT TRP6C 1 0 PERMIT 43.2 ****** PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT-NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify under penalty of law that this document and all attachments were prepared under my / TELEPHONE DATE________________________________________- rection or suparvision in accordance with a system designad to asaure that qualified personn I /John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the / e V President information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 12 06 12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SINTURE OF PRINCIPAL EXECUTIVE -including the possibility of fine and imprisonment for knowing violations. " F -ER OR AUTHORIZED AGENT YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)Toxicity sampling was performed May 6 -11. The report is included as an attachment. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000..qINTEROFFICE OPS-5N-SQN) SODDY -DAISY TN_37384 Facility. TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATIN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form DISCHARGE MONITORING REPORT (DMR)(SUBR 01) OMBI TN06450IZ 103 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER] LOW VOL. WASTE TREATMENT POND MONITORING PERIOD EFFLUENT Approved.No- 2040-0004 IAR MODAY IYEAR IMOI DAY From[ 12 os 05oi 0 To0 12 1 0 i 31 NO DISCHARGE Zý *NOTE: Read instructions before completing this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7 9 12 15131 GRAB MEASUREMENT 00400 1 0 PERMIT ***6 9 SU THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 7 9 19 0 2 1 31 GRAB MEASUREMENT 00530 1 0 PERMIT **

30 100 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH OIL AND GREASE SAMPLE ** <5 <6 19 0 2 / 31 GRAB MEASUREMENT 00556 1 0 1 PERMIT is 15 20 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.038 1.216 03 -0 31 131 RCORDR TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD SEE RCORDR EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX _ _PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the President information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-70(1 12 06 12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, ýKIAATU q.ýOF PRINCIPAL EXECUTIVE -including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)Outfall 103 TRC analysis collected during discharge of Steam Generator Process Water from drilling core pilings is included as an attachment. EPA Form 3320-i (REV 3199) Previous editions may be used Page I of I DMR Attachment Outfall 103: TRC Sample collected during discharge of Steam Generator Project discharge of process water from drilling core pilings. Reference DMR Cover Letter.Outfall 103 DatelTime Collected TRC Analysis Date/Time Analyst 511512012 1 11:10 0.03 mglL 511512012111:24 MLW PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000-I-NTEROFFCE OPS-5N-SQN) SODDY -DAISY_ TN 37384 Facty TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTL ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)(SUBR 01)TN0026450 I 110 G IF-FINAL TPERMI NUMBER ] DISCHARGE NUMBER RECYCLED COOLING WATER Form Approved.OMB No. 2040-0004 MfII1IT'%DIKIfl' DriIt-lrl I EFFLUENT I YEARI MO I DAY I YEARAY NO DISCHARGE From 1 12 05 01 To 12 05 31 NOTE: Read instructions before completinp this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 04 CENTIGRADE MEASUREMENT 00010 1 0 PERMIT REPORT DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT ... DAILY MX UoUS TEMPERATURE, WATER DEG. SAMPLE **04 CENTIGRADE MEASUREMENT 00010 Z 0 PERMIT ****30.5 DEG C CONTIN CALCTD INSTREAM MONITORING REQUIREMENT DAILYMX UOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ***04 UPSTRM DEG.C MEASUREMENT 00016 1 0 PERMIT * *5 DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 03 50050 1 0 PERMIT Req. Mon. MGD CONTIN RCORDR EFFLUENT GROSS VALUE REQUIREMENT DAILY MX' UOUS CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 50060 1 0 PERMIT *0.1 0.1 MGIL Five per CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX Week TEMPERATURE -C, RATE OF SAMPLE 04 CHANGE MEASUREMENT 04 82234 1 0 PERMIT 2 DEG C ******** CONTIN CALCTD REQUIREMENT " X EFFLUENT GROSS VALUE DAILY MX SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my.-" TELEPHONE DATE direction or super-ision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the e ice resident 4 information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 12 06 12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNAT RE (1, PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICEROR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all aftachments here)No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of I PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000-- INTEROFFCE OPS-5N-SQN) SODDY -DAISY, 1N.37384 _Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)DISCHARGE MONITORING REPORT (DMR)TN0026450ZZ 110 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD Y EAR MOM DAY Y From 12To 12 31 MAJOR Form Approved.(SUBR 01) OMB No. 2040-0004 F -FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE [] ...NOTE: Read instructions before completinq this'form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS 1C25 STATRE 7DAY CHR SAMPLE **CERIODAPHNIA MEASUREMENT 23 TRP3B 1 0 0 PERMIT 43.2 PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ** 23 PIMEPHALES MEASUREMENT 23 TRP6C 1 0 0 PERMIT 432 PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT .MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted, Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the SquPresident information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 ice 12e06d12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED _CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)No Discharge this Period EPAFom .3.-. (RV.....re.os...ton.m y.e.se.P ge1.f.EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of I PERMIT-EE NAME/ADDRESS (Include Facility Name/Location if DifferentJ Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000_ -JLINTEROFFICE OPS-5N-SQ.N) SODDY -DAISY TN_37384 Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 TN0026450 118 G I F-FINAL PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER I MONITORING PERIOD 1 EFFLUENT iYEAR I MO 1 =7.. 1 MO IDA YEAR M A YEAR O DAY

NO DISCHARGE[7 From 12 05 01 To 12 105 131 NOTE: Read instructions before completino this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE 19 MEASUREMENT 19 00300 1 0 PERMIT 2 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 19 00530 1 0 PERMIT 100 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT " DAILY MX. WEEK SOLIDS, SETTLEABLE SAMPLE ***25 MEASUREMENT 00545 1 0 PERMIT
MUL ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 03***TREATMENT PLANT MEASUREMENT 03 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD * *. .* ONCE/ -ESTIMA REQUIREMENT M V A. = .......; ... AC ..EFFLUENT GROSS MOAG DAILY MX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the e sident information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 12 06 12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE --PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations. OFFICE R AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED ______________________________ _________________CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.EPA Form 3320-1 (REV 3199) Previous editions may be used Page I of 1 June 11, 2012 Bradley M. Love, OPS 5N-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, MAY, 2012 Per your request, I have also submitted an electronic copy of the subject report. Outfall 101 samples collected May 06-1 1, 2012, showed no toxic effects to fathead minnows or daphnids.The resulting IC 2 5 values for both species were> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV-treated samples.Please call me at your convenience if you have any questions or comments following your review of the report.DoadW. Sno Manager, Environmental Engineering Field Team-West CTR 2L -M Attachment Cc (Attachment): R.M. Sherrard, PSC IX -C (Electronic copy)EDMS, MPB I E -M (Electronic copy) TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: June 11, 2012 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA 2. County / State: Hamilton / Tennessee 3. NPDES Permit #: TN0026450 4. Type of Facility: Nuclear-Fueled Electric Generating Plant 5. Design Flow (MGD): 1,579 6. Receiving Stream: Tennessee River (TRM 483.6)7. 1Q10:3,491

8. Outfall Tested: 101 9. Dates Sampled: May 06- 11, 2012 10. Average Flow on Days Sampled (MGD): 1715.29, 1713.71, 1700.72 11. Pertinent Site Conditions:

Production / operation data will be provided upon request.12. Test Dates: May 08 -15, 2012 13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)Daphnids (Ceriodaphnia dubia)15. Concentrations Tested (%): Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100 Pimephalespromelas:

UV treated Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100 16. Permit Limit Endpoint (%): Outfall 101: IC 2 5 = 43.2%17. Test Results: Outfall 101: Pimephales promelas: IC 2 5 > 100%Ceriodaphnia dubia: IC5 > 100%UV treated Outfall 101: Pimephales promelas: IC2 5> 100%Page 1 of 110

18. Facility Contact: Brad Love Phone#: (423) 843-6714 19. Consulting

/ Testing Lab: Environmental Testing Solutions, Inc.20. Lab Contact: Jim Sumner Phone #: (828) 350-9364 21. TVA Contact: Donald W. Snodgrass Phone #: (256) 386-2787 22. Notes: Outfall 101 samples collected May 06 -11, 2012, showed no toxic effects to fathead minnows or daphnids. The resulting IC 2 5 values, for both species, were >100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.Page 2 of 110 METHODS

SUMMARY

Samples: 1. Sampling Point: Outfall 101, Intake 2. Sample Type: Composite 3. Sample Information: Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET)ID Collected Received (C) (mg/L) Last Used By 101 05-06-12 0855 to 05-07-12 1429 1.8, 1.8t <0.10 05-08-12 1130.05-07-12 0755 05-09-12 1030 05-06-12 0910 to 05-08-12 1130 Intake 05-07-12 0810 05-07-12 1429 1.0 <0.10 05-09-12 1030 05-07-12 0810 05-09-12 1030 101 05-08-12 0655 to 05-09-12 1300 1.6, 1.7t <0.10 05-10-12 1053 05-09-12 0555 05-11-12 1030 05-08-12 0710 to 05-10-12 1053 Intake 050912 0610 05-09-12 1300 1.6 <0.10 05-11-12 103 05-09-12 0610 05-11-12 1030 05-12-12 1030 101 05-10-12 0655to 05-11-12 1225 0.5,0.6t <0.10 05-13-12 1032 05-11-12 0555 05-14-12 1030 05-10-12 0710 to 05-12-12 1030 Intake 05-10-12 0610 05-11-12 1225 2.1 <0.10 05-13-12 1032 05-11-12 0610 05-14-12 1030*TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath.Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.Page 3 of 110 Test Organisms: Pimephales promelas Aquatox, Inc.19.2 -19.5 hours old Ceriodaphnia dubia In-house Cultures< 24-hours old 1. Source: 2. Age: Test Method Summary: 1. Test Conditions: Static, Renewal Static, Renewal 2. Test Duration: 7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic 3. Control / Dilution Water: Moderately Hard Synthetic 4. Number of Replicates: 4 5. Organisms per Replicate: 10 6. Test Initiation: (Date/Time) Outfall 101 UV Treated Outfall 101 7. Test Termination: (Date/Time) Outfall 101 UV Treated Outfall 101 10 1 05-08-12 1130 ET 05-08-12 1112 ET 05-15-12 1030 ET 05-15-12 1015 ET 05-08-12 0935 ET 05-15-12 0842 ET Mean = 25.0°C (24.7 -25.3 0 C)8. Test Temperature: Outfall 101: Mean = 24.8 0 C (24.5 -25. 1°C)Test Temperature: UV-Treated Outfall 101: (Aean = 24.8°C 24.3 -25.2 0 C)9. Physical / Chemic Measurements: al Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).10. Statistics: Page 4 of 110 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)I Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.(Genus species) (Type / Duration)Conducted May 08 -15, 2012 using effluent from Outfall 101.Test Percent Surviving Solutions (time interval used -day s)(% Effluent) 1 21 3 4 5( 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 98 98 98 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg)Test Solutions (replicate numbe r)(%Effluent) 1 2 3 4 Mean Control 0.658 0.686 0.707 0.778 0.707 10.8% 0.624 0.648 0.699 0.647 0.655 21.6% 0.629 0.717 0.594 0.716 0.664 43.2% 0.678 0.674 0.642 0.674 0.667 86.4% 0.660 0.604 0.657 0.648 0.642 100.0% 0.621 0.634 0.586 0.682 0.631 Intake 0.734 0.634 0.693 0.716 0.694 IC 2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*Permit Limit: 43.2%Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NAA*TUa = 100I/LC 5 0: TUc = 100/IC 2 5 Page 5 of 110 0-p TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.(Genus species) (Type / Duration)Conducted May 08 -15, 2012 using effluent from Outfall 101.Percent Surviving Test (time interval used -days)Solutions (% Effluent) 1 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)Test Soluetis Data (replicate number)(%Effluent) 1 2 [ 3 f4 1 5 6 17 J 8 [ 9 10 [Mean Control 33 32 30 31 36 30 33 34 31 29 31.9 10.8% 33 33 30 33 31 32 28 35 35 32 32.2 21.6% 36 32 36 33 31 32 35 32 35 33 33.5 43.2% 35 31 33 39 33 30 35 37 33 33 33.9 86.4% 34 36 32 35 37 35 34 34 33 35 34.5 100.0% 37 36 35 35 37 38 33 36 35 36 35.8 IC 2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*Permit Limit: 43.2%Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA*TUa = 100/LC 5 0: TUc = 100/IC 2 5 Page 6 of 110 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.(Genus species) (Type / Duration)Conducted May 08 -15, 2012 using water from Intake Percent Surviving Test (time interval used -days)Solutions 1 1 1 (% Effluent) 5 6 7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)(%_Effluent) Data (replicate number)(%Effluent) 1 2 3 4 5 f 6 7 8 9 10 Mean Control 30 33 33 30 31 29 32 31 31 26 30.6 Intake 33 35 29 32 31 36 32 31 33 31 32.3 IC 2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA*TUa = 100/LC 5 0: TUc = 100/IC 2 5 Page 7 of 110 TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.(Genus species) (Type / Duration)Conducted May 08 -15, 2012 using effluent from UV Treated Outfall 101.Test Percent Surviving Solutions (time interval used -days)(% Effluent) 1 2 3 4 5 [6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake ioo 100 98 98 98 98 98" Mean Dry Weight (mg)Test Soluetis (replicate number)(%Effluent) 1 2 3 4 Mean Control 0.591 0.647 0.507 0.599 0.586 10.8% 0.646 0.621 0.616 0.698 0.645 21.6% 0.553 0.622 0.659 0.663 0.624 43.2% 0.565 0.609 0.634 0.606 0.604 86.4% 0.537 0.573 0.610 0.560 0.570 100.0% 0.673 0.680 0.676 0.653 0.671 Intake 0.597 0.592 0.561 0.704 0.614 IC 2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*95% Confidence Limits: Upper Limit: NA Lower Limit: NA*TUa = 100/LC 5 0: TUc = 100/IC 2 5 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)Species Date Time Pimephalespromelas May 08 -15, 2012 1236 Ceriodaphnia dubia May 08 -15, 2012 0908 I Duration I T oxicant j Results (IC 2 5)KC1 0.77 g/L NaC1 1.06 g/L Page 8 of 110 (D PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pimephalespromelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed May 08-15, 2012.Test Sample ID Temperture (C) Dissolved Oxygen (g/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (tmihos/cm) (mg/L CaCO 3) (mg/L CaCO 3) Chlorine (nmg/L)Control 24.8 24.7 7.6 7.2 7.96 7.72 310 63 88 24.8 -24.9 24.6 -25.0 7.4 -7.8 6.4 -8.0 7.85 -8.06 7.49 -7.90 294 -327 62 -63 87 89 10.8% 24.9 24.7 7.8 7.2 7.97 7.68 293 24.8 -25.0 24.5 -24.9 7.6 -8.0 6.4 -7.9 7.89 -8.10 7.49- 7.86 283 -306 24.9 24.7 7.8 7.1 7.98 7.68 283 21.6%21"6% 24.8 -25.0 24.5- 24.9 7.6 -8.0 6.5 -7.9 7.89 -8.10 7.49 -7.86 276 -291 4 24.9 24.7 7.8 7.2 7.96 7.71 260 24.8 -25.0 24.6 -25.1 7.6 -8.0 6.5 -8.0 7.86 -8.11 7.47 -7.90 253 -265 8 24.9 24.7 7.9 7.2 7.95 7.72 213 86.4%100.0% 25.0 24.7 7.9 7.2 7.94 7.73 195 80 81 < 0.10 24.8 -25.0 24.5 -25.0 7.6 -8.0 6.2 -8.0 7.76 -8.16 7.42 -7.92 188 -199 79 -81 81 -81 <0.10- <0.10 Intake 25.0 24.8 7.9 7.3 8.06 7.75 190 78 80 <0.10 24.9 -25.1 24.7 -25.1 7.7 -8.1 6.7 -8.0 7.95 -8.16 7.53 -7.93 182 -195 77 -79 79 -81 <0.10- <0.10 Control 24.8 25.0 7.6 7.8 7.96 7.95 310 63 88 24.7 -25.0 24.9 -25.2 7.4 -7.8 7.6 -8.0 7.85 -8.06 7.87 -8.02 294 -327 62 -63 87 -89 10.8% 24.9 25.0 7.8 7.8 7.97 7.95 293 24.7 -25.2 24.8 -25.3 7.6 -8.0 7.6 -8.0 7.89 -8.10 7.86 -8.03 283 -306". 24.9 25.0 7.8 7.8 7.98 7.95 283 24.7 -25.2 24.8 -25.2 7.6 -8.0 7.6 -8.0 7.89 -8.10 7.86 -8.02 276 -291 24.9 25.0 7.8 7.8 7.96 7.95 260 43.2%24.8 -25.2 24.7 -25.2 7.6 -8.0 7.6 -8.1 7.86 -8.11 7.85 -8.03 253 -265 86.4% 24.9 25.0 7.9 7.8 7.95 7.98 213___ 24.8 -25.2 25.0 -25.2 7.6 -8.0 7.6 -8.0 7.81 -8.12 7.86 -8.07 210 -218 100.0% 25.0 25.0 7.9 7.8 7.94 8.00 195 80 81 < 0.10 24.8 -25.2 24.8 -25.2 7.6 -8.0 7.6 -8.1 7.76 -8.16 7.87 -8.06 188 -199 79 -81 81 -81 <0.10- <0.10 Intake 25.0 25.0 7.9 7.9 8.06 7.98 190 78 80 <0.10 24.8 -25.2 24.8 -25.2 7.7 -8.1 7.8 -8.1 7.95 -8.16 7.87 -8.03 182 -195 77 -79 79 -81 <0.10- <0.10 Overall temperature (C)Pimephales promelas Ceriodaphnia dubia Average 24.8 25.0 Minimum 24.5 24.7 Maximum 25.1 25.3 I.lb 0 PHYSICAIJCHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pimephales promelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 101 perfbrmed May 08-15, 2012.Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) PH (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (gmhos/cm) (mg/L CaCO 3) (mg/L CaCO 3) Chlorine (mg/L)Control 24.8 24.7 7.9 7.3 7.97 7.72 307 63 88 24.7 -24.9 24.5 -24.8 7.6 -8.0 6.5 -8.0 7.88 -8.07 7.51 -7.91 300 -316 62 -64 87 -89 10.8% 24.9 24.6 7.9 7.2 7.97 7.70 296 24.8 -25.0 24.4 -24.9 7.6 -8.0 6.6 -7.9 7.88 -8.09 7.50 -7.85 283 -305 21.6% 24.9 24.7 7.9 7.2 7.97 7.71 283 24,8 -25.0 24.5 -24.9 7.6 -8.0 6.5 -8.0 7.89 -8.10 7.50 -7.90 269 -291 43.2% 24.9 24.6 7.9 7.2 7.96 7.71 260 24.8 -25.1 24.5 -24.8 7.6 -8.0 6.5 -7.9 7.86 -8.11 7.50 -7.89 248 -266 86.4% 24.9 24.7 7.9 7.2 7.94 7.74 216 24.8 -25.1 24.6 -24.9 7.5 -8.0 6.5 -7.9 7.79 -8.13 7.50 -7.90 207 -221 25.0 24.6 7.9 7.2 7.94 7.74 198 79 81 < 0.10 100. 24.8 -25.2 24.3 -24.8 7.6 -8.1 6.5 -7.9 7.76 -8.15 7.50 -7.91 189 -201 79 -79 81 -81 <0.10 -<0.10 Intake 25.0 24.7 8.0 7.2 8.02 7.77 192 78 80 <0.10 24.8 -25.2 24.5 -24.9 7.6 -8.2 6.6 8.0 7.94 -8.16 7.52 -7.95 184 -196 77 -79 79 -81 <0.10 -<0.10 Overall temperature (C)Pimephales promelas Average 24.8 Minimum 24.3 Maximum 25.2 j

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected May 06 -11, 2012, showed no toxic effects to fathead minnows or daphnids.

The resulting IC 2 5 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.Page 11 of 110 Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS 1. Pimephales promelas Tests were conducted according to EPA-82 1 -R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.2. Ceriodaphnia dubia Tests were conducted according to EPA-82 1 -R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL 1. Pimephales promelas None 2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS 1. Pimephales promelas None 2. Ceriodaphnia dubia None Page 12 of 110 PHYSICAL AND CHEMICAL METHODS 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments:

All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.3. Temperature was measured by SM 2550 B.4. Dissolved oxygen was measured by SM 4500 0 G.5. The pH was measured by SM 4500 H+ B.6. Conductance was measured by SM 2510 B.7. Alkalinity was measured by SM 2320 B.8. Total hardness was measured by SM 2340 C.9. Total residual chlorine was measured by ORION Electrode Method 97-70.QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC 2 5 values in g/L KC1 or NaCl.2. Standard Toxicant:

Potassium Chloride (KC1 crystalline) for Pimephales promelas.Sodium Chloride (NaCI crystalline) for Ceriodaphnia dubia.3. Dilution Water Used: Moderately hard synthetic water.4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.Page 13 of 110 REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-82 I-R-02-013 (October 2002).3. Standard Methods for the Examination of Water and Wastewater, 21 st Edition, 2005.4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).Page 14 of 110 Sequoyah Nuclear Plant Biomonitoring May 08 -15, 2012 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 110 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 11, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 03/12/1998 0.016 ------03/13/1998 0.015 ------03/14/1998 0.013 ------03/15/1998 0.030 ------03/16/1998 0.013 ------03/17/1998 0.020 ------03/18/1998 0.018 ------09/08/1998 0.015 -0.014 0.005 --0.021 09/09/1998 0.003 -0.031 0.011 ---09/10/1998 0.014 -0.060 0.021 ---09/11/1998 0.013 -0.055 0.019 ---09/12/1998

< 0.001 -0.044 0.015 ---09/13/1998 < 0.001 -0.044 0.015 ---09/14/1998 0.008 -0.044 0.015 -02/22/1999 < 0.001 ----02/23/1999 0.005 ----02/24/1999 0.009 ----02/25/1999 0.012 ----02/26/1999 0.008 ----02/27/1999 < 0.001- --02/28/1999 < 0.001 08/18/1999 -0.015 0.069 0.024 0.006 08/19/1999 -0.012 0.068 0.024 --08/20/1999 -0.023 0.070 0.024 -0.120 08/21/1999 -0.022 0.068 0.024 ---08/22/1999 -0.022 0.068 0.024 ---08/23/1999 -0.025 0.068 0.024 0.006 --08/24/1999 -0.016 0.067 0.023 0.020 --Page 16 of 110 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998-May 11, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/31/2000 -< 0.002 0.026 0.009 02/01/2000 -0.011 0.026 0.028 -02/02/2000 -0.028 0.026 0.009 0.006 02/03/2000 -0.008 0.027 0.009 --02/04/2000 -0.006 0.027 0.009 0.005 0.109 02/05/2000 -< 0.002 0.027 0.009 --02/06/2000 -< 0.002 0.027 0.009 -07/26/2000 -< 0.0057 0.055 0.019 -07/27/2000 -0.019 0.055 0.019 -07/28/2000 -0.0088 0.053 0.018 0.004 0.108 07/29/2000 -< 0.0088 0.055 0.019 --07/30/2000 -< 0.0076 0.055 0.019 ---07/31/2000 -< 0.0152 0.055 0.019 0.006 --08/01/2000 -< 0.0141 0.055 0.019 0.005 --12/11/2000 -0.0143 0.025 0.020 0.005 --12/12/2000 -0.0092 0.025 0.020 0.005 --12/13/2000 -< 0.0120 0.025 0.020 ---12/14/2000 -< 0.0087 0.025 0.020 ---12/15/2000 -0.0120 0.025 0.020 0.005 --12/16/2000 -< 0.0036 0.025 0.020 ---12/17/2000 -< 0.0036 0.025 0.020 -08/26/2001 -0.017 0.06 0.021 0.006 08/27/2001 -<0.0096 0.06 0.021 0.005 0.021 08/28/2001 -<0.0085 0.06 0.021 --08/29/2001 -<0.0094 0.059 0.020 0.005 0.021 08/30/2001 -<0.0123 0.06 0.021 0.005 -08/31/2001 -<0.005 0.059 0.020 -I 11/25/2001 -<0.0044 ---11/26/2001 -<0.0119 0.024 0.02 0.005 11/27/2001 -0.0137 0.023 0.019 0.007 11/28/2001 -<0.0089 0.022 0.019 0.006 11/29/2001 -0.0132 0.024 0.02 0.007 11/30/2001 -< 0.0043 0.024 0.02 -I 12/09/2001 -<0.0042 --12/10/2001 -<0.0042 12/11/2001 -<0.0104 ---12/12/2001 -0.0128 0.024 0.02 0.008 12/13/2001 -<0.0088 0.024 0.02 -12/14/2001 -0.0134 0.024 0.02 0.007 1 Page 17 of 110 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 11, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/02/2002 -< 0.0079 0.023 0.02 0.006 -01/03/2002 -< 0.0042 0.023 0.014 --01/04/2002 -0.0124 0.024 0.014 0.009 -01/05/2002 -< 0.0042 ---01/06/2002 -< 0.0042 --- -01/07/2002 -< 0.0089 0.024 0.014 0.006 --02/24/2002 -< 0.004 ----02/25/2002 -< 0.004 0.023 0.023 --02/26/2002 -0.0143 0.023 0.023 0.007 -02/27/2002 -< 0.0041 0.023 0.023 --02/28/2002 -< 0.0041 0.024 0.008 -03/01/2002 -< 0.0041 0.024 0.008 1 1 05/05/2002

05/06/2002

--0.058 0.02 0.014 --05/07/2002 --0.058 0.02 0.015 --05/08/2002 --0.056 0.019 ---05/09/2002 --0.057 0.02 0.014 --05/10/2002 --0.056 0.019 -- I 08/04/2002 -<0.0058 ---08/05/2002 -<0.0058 0.053 0.018 -0.025 08/06/2002 -0.0092 0.053 0.018 ---08/07/2002 -<0.0107 0.055 0.019 0.007 --08/08/2002 -<0.0061 0.055 0.019 ---08/09/2002 -0.0152 0.054 0.018 0.008 -10/06/2002 -<0.00497 -----10/07/2002 -0.0153 0.054 0.018 0.009 --10/08/2002 -<0.0092 0.054 0.018 0.007 --10/09/2002 -0.0124 0.053 0.018 0.009 --10/10/2002 -0.0134 0.054 0.018 0.009 --10/11/2002 -<0.0042 0.054 0.018 -01/12/2003 -<0.0035 ---01/13/2003 -<0.006 0.025 0.019 0.009 --01/14/2003 -<0.01 18 0.026 0.020 --01/15/2003 -<0.0063 0.026 0.020 0.009 --01/16/2003 -<0.0034 0.026 0.020 --01/17/2003 -<0.0034 0.026 0.009 ---04/06/2003 -<0.0073 -----04/07/2003 -<0.0189 -0.021 ---04/08/2003 -<0.0117 -0.021 ---04/09/2003 -<0.0139 -0.021 0.016 --04/10/2003 -<0.0113 -0.021 0.018 --04/11/2003 -<0.0073 -0.022 -Page 18 of 110 Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 11, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 06/15/2003 -< 0.0045 -----06/16/2003 -< 0.0037 0.057 0.020 --0.022 06/17/2003 -< 0.0048 0.041 0.014 --0.024 06/18/2003 -< 0.0048 0.041 0.014 --0.024 06/19/2003 -< 0.0085 0.058 0.020 --0.025 06/20/2003 -< 0.0048 0.058 0.020 --0.025 08/03/2003 -<0.0050 -----08/04/2003 -<0.0050 0.058 0.020 ---08/05/2003 -<0.0051 0.057 0.020 --0.025 08/06/2003 -<0.0084 0.057 0.020 --0.025 08/07/2003 -0.0129 0.057 0.020 --0.024 08/08/2003 -0.0153 0.057 0.020 0.009 --10/05/2003 -<0.0043 0.057 0.020 ---10/06/2003 -<0.0043 0.057 0.020 --0.025 10/07/2003 -<0.0090 0.057 0.020 --0.025 10/08/2003 -<0.0106 0.057 0.020 --0.025 10/09/2003 -0.0181 0.026 0.022 --0.025 10/10/2003 -0.0183 0.026 0.024 0.009 --02/01/2004 -0.0093 0.027 0.009 --02/02/2004 -<0.0034 0.026 0.009 02/03/2004 -<0.0034 0.026 0.009 -02/04/2004 -0.0124 0.026 0.009 0.009 -02/05/2004 -<0.0034 0.026 0.009 --02/06/2004 -0.0105 0.026 0.009 0.010 -05/04/2004 -<0.0123 0.026 0.019 --0.025 05/05/2004 -<0.0144 0.026 0.014 0.009 -0.025 05/06/2004 -<0.0146 0.037 0.013 --0.025 05/07/2004 -0.0227 0.058 0.020 0.009 -0.025 05/08/2004 -0.016 0.060 0.021 ---05/09/2004 -<0.0104 0.058 0.020 -07/04/2004 -0.0217 0.057 0.019 --07/05/2004 -<0.0085 0.057 0.020 0.009 --07/06/2004 -<0.0077 0.058 0.020 --0.031 07/07/2004 -0.0252 0.056 0.019 --0.031 07/08/2004 -0.0223 0.057 0.019 0.009 --07/09/2004 -0.0182 0.057 0.020 0.009 --Page 19 of 110 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 11, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M Hypochlorit mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L e TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L EO/PO TRC 11/07/2004 -<0.0187 0.000 0.014 1- -11/08/2004 -<0.0192 0.047 0.030 -----11/09/2004 -<0.0233 0.048 0.016 --0.041 --11/10/20041 <0.0149 0.047 0.016 --0.041 --11/11/20041 <0.0149 0.049 0.017 --0.043 --11/12/20041 -<0.0253 0.048 0.017 -0.042 -02/06/2005 -<0.0042 0.028 0.010 E -02/07/2005 -<0.0116 0.028 0.010 -0.007 02/08/2005 -<0.0080 0.028 0.010 -I 02/09/2005 _ _0.0199 0.028 0.010 [02/10/2005 -<0.0042 0.028 0.010 02/11/2005 -0.0155 0.028 0.010 -0.007 06/05/2005 -0.0063 -----06/06/2005 -0.0043 ----0.037 06/07/2005 -0.0103 ---0.037 06/08/2005 -0.0295 ---0.037 06/09/2005 -0.0129 ---06/10/2005 -0.0184 --07/17/2005 -0.0109 0.026 0.009 --07/18/2005 -0.0150 0.026 0.009 -0.036 07/19/2005 -0.0163 0.026 0.009 --0.036 07/20/2005 -0.0209 0.026 0.009 -0.014 0.036 07/21/2005 -0.0242 0.026 0.009 ---07/22/2005 -0.0238 0.054 0.018 0.014 10/30/2005 -0.0068 ---10/31/2005 -0.0112 ---11/01/2005 -0.0104 ---0.035 11/02/2005 -0.0104 ---0.036 11/03/2005 -0.0117 ---0.036 11/04/2005 -0.0165 -0.035 11/14/2005 -0.0274 -11/15/2005 -0.0256 11/16/2005 -0.0234 11/17/2005 -0.0231 11/18/2005 -0.0200 11/19/2005 -0.0116 1 1 1 Page 20 of 110 Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-May 11, 2012 Date Sodium Towerbromr PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M MSW Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L 101 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L TRC EO/PO Phosphate 11/12/2006 -0.0055 ---11/13/2006 -0.0068 --0.037 11/14/2006 -0.0143 --0.037 11/15/2006 -0.0068 --0.037 11/16/2006 -0.0267 --0.037 11/17/2006 -0.0222 ---11/26/2006 -0.0188 --11/27/2006 -0.0138 --11/28/2006 -0.0120 --11/29/2006 -0.0288 --11/30/2006 -0.0376 --12/01/2006 -0.0187 --05/28/07 -----0.015 05/29/07 -----0.036 0.015 05/30/07 -0.0084 --0.017 0.036 0.015 05/31/07 -0.0103 ---0.036 0.015 06/01/07 -0.0164 --0.017 0.036 0.015 06/02/07 -0.0305 ----0.015 12/02/07 -0.0241 --------12/03/07 -0.0128 --------12/04/07 -0.0238 --------12/05/07 -0.0158 --------12/06/07 -0.0162 --------12/07/07 -0.0175 -------04/13/08 -0.0039 --------04/14/08 -0.0124 -------04/15/08 -0.0229 -------04/16/08 -0.0143 -------04/17/08 -0.0120 -------04/18/08 -0.0149 -------10/26/08 -0.0260 --------10/27/08 -0.0151 ----0.017 --10/28/08 -0.0172 -----0.041 -10/29/08 -0.0154 ----0.018 0.041 0.030 10/30/08 ------0.041 0.030 10/31/08 -0.0086 ----0.041 0.030 Page 21 of 110 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 11, 2012 Date Sodium Towerbron PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg/L Quat mg/L TRC Phosph EO/PO Quat Phosphate ate 02/08/09 -0.0197 -----0.017 02/09/09 -0.0237 -----0.017 02/10/09 -0.0104 -----0.021 02/11/09 -0.0155 -----0.017 -02/12/09 -0.0106 -----0.017 -02/13/09 -- -05/10/09 -0.0129 --05/11/09 -0.0415 --0.0446 -05/12/09 -0.0053 --0.0396 -05/13/09 -0.0049 --- 0.0396 -05/14/09 -<0.0141 --0.0397 -05/15/09 -<0.0160 11/15/09 -0.025 11/16/09 -0.0152 11/17/09 -0.0255 -11/18/09 -0.0306 -11/19/09 -0.0204 -11/20/09 -0.0093 -05/09/10 -0.0192 05/10/10 -0.0055 05/11/10 -0.0100 -0.039 --05/12/10 -0.0171 -0.039 --05/13/10 -0.0041 -0.039 --05/14/10 -0.0099 ------0.039 --Page 22 of 110 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 11, 2012 Date Sodium Towerbron PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg/L Quat mg/L TRC Phosph EO/PO Quat Phosphate ate 10/31/10 -----11/01/10 -0.0122 ---11/02/10 -0.0112 ---11/03/10 -0.0163 ---11/04/10 -0.0107 --- -11/05/10 -0.0132 --05/01/2011 --05/02/2011 --0.04 --05/03/2011 ---0.04 --05/04/2011 -0.0155 -0.04 --05/05/2011 -0.0179 -0.04 --05/06/2011 -0.0089 ---11/06/2011 -0.0168 11/07/2011 -0.0225 11/08/2011 -0.0141 11/09/2011 -0.0239 11/10/2011 -0.0242 11/11/2011 -0.0231 05/06/2012 --05/07/2012 -05/08/2012 --0.041 05/09/2012 -0.0145 0.041 -05/10/2012 -0.0298 0.041 -05/11/2012 -0.0174 -Page 23 of 110 Sequoyah Nuclear Plant Biomonitoring May 08 -15, 2012 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 24 of 110 BIOMONITORING CHAIN OF CUSTODY RECORD Page _1- of -I-Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): 'Gen ral Comments* Facility Sampled: Sequoyah NP 28801 C ... 1 97..*z Vir 4W --+# .. m NPDES Number. TN0026450 Phone: 828-350-9364 IDawo =I Colce BYL,,, g;,,.- Fax: 828-350-9368 CollectedBy FieldI Identificati Number & Flow Rain Event? i ..i Sample Description Volume (MGD) (rka TopDopn .a.orrtJ.& Date Time SgYes "tYes. No TraRc By ySiature D tep7 -y -Time App. r Npimber =1 I' ichsance., SQN-101-TOX Comp $Vdn 2(2.Sgal) 9 4 SQN-INT.TOX Comp 1(2.52 gal 0 .A________________________Sample Cutdy -Fill In From Top Down $o-v ( s e~t-s %. aPiTAcr, sow9fIvts otCem Relinquished By (Signature): Date/Time Received By (Signature): Date/Time S.mnStQ_____________ --h cr- 12 1Dt e r~_________ os-c-tx L"- e- rw a-al I- V4A e Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the followirhg Monday.

ETS CD ETS Pa. rL-Whole Effluent Toxicity Sample Receipt Log*Swuphleteperaturaper~funnd eusng Sample Reociing Thcramoeter SN 6338.0 Date Time Reetsved Reeived Sample Projee Sample Sample anmeanddeeptln Sltate C=memal recgedd by~le from tempamlui CC) member_________________

_________05,07-12 1429 J. Sunmne TVACauier 1-8/1.8 7875 120507.01 TVA -$uaye Nule~ar Plemt -OatMl 101 --T'N 05-07-12 1429 L. Sunmer TVACowi.' 1.0 7875 120507.02 7vA-Se- hNode. Plant- nakem T14 05-07-12 1451 1. Sumner TVACourier 1-7 7876 120507.03 TVA -WaftsBarNuleaPleat-OullI1101 7N 05-07-12 1451 3 Swnw IVACowier 3.7 7876 120507.04 rVA -WatlsBerackaer Plat-nt e-I 101 T7 05-07-12 1451 7. Su .VA Coier 2876 120507.05 rVA -W Baterlu ew Pleat-Outfa]l 113 T14 054-7-12 1451 L. SMn!e TVACourier

3. 7876 120507.06 1VA-WelsBar 4ucelaMtntak 113 T/N Pleat SOP 04. -Exf"bit 041. rawbio 01-03-12 BIOMONITORING CHAIN OF CUSTODY RECORD.-Client: TVA Environmental Testing Solution, Inc.I Delivered By (Circle One): O-9's Project Name: Sequoyah NP Toxicity 351 Depot Street.FedEx UPS Bus P.O. Number: NA Asheville, NC Facility Sampled: Sequoyah NP 28801 NPDES Number: TN0026450 Phone: Fax: 828-350-9364 828-350-9368 Collected By: Dustin Instructions:

Clients should fill in all areas except those in the "Laboratoy Use" block. Biomonitoring samples are preserved by storing them at 6*C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. ET~i f--Whole Effluent Toxicity Sample Receipt Log Pa~eJ-N*San&l tempeateare pesftmead using Sample Receiving Thermometer. SN 6338.-9, Date Thee R-*vd ecv Sample PrOetle Samsple Sample ame mad deacrsiomn State Coements recebved received fbam t mprature C- namber 05-09-12 1002 K. Keman Fed -Ex 1.4 7896 120509.01 C"01l ERI -AAF Mciuqy Jtemnational" NC " 05-09-12 1002 K. Keamm Fed -Ex 2.0 7899 120509,02 Bladenboro WWTP NC 05-09-12 1002 K. Kammn Fed -E 1. 7 700 120509.03 Duke B p- Be[ew, ek Stema Station NC _05-09612 1002 K. Keeman Fed-&. 1.5 7901 120509.04 Progress eww -CaPlFearS,]L. NC 05-09-12 1002 K. Keean Fed -Ex 15 7901 120509.05 EtM as Enera -Fae Fear &B, -p NC 05-09-12 11002 LKleman Fed-Eut 0.7 7902 120509.06 a of(adonia-DallasWWT7 NC 05-09-12 1002 K. Keenan Fed- E 5.8 903 120509.07 UnitedWalter -Endeld WWrP ... ..._NC 05-09-12 1002 K Kamn Fed -EX 23 7904 120509.08 DukEe -MI( Stema Station NC 05-09-12 1002 K. Keman Fed -Ex 146 7905 120509.09 AmnxwReumnms -REleib CC NC -, 05-09-12 1002 IL Keenan Fed- Ex 2,6 7906 120509.10 Bell Sw- Salelt Acres MHP ..NC_....1002 K. Yeen Fed -Ex 5.8 7907 120509.11 United Water -Scotlmd Neck NC 05-09-12 1002 K. Kenamm Fed- Ex 1.7 7908 120509.12 Duke En -Mcuire NS -OutfiO005 NC 05-09-12 102 KM Keenan Fed- Eu 2.4 7909 120509.13 Duke Enew -Mcons NS -Ouffall 001 NC 05-.09-12 ,002 K =eeea Faed-EX 1.7 7910 120509.14 Duke eW-Mcuire NS-O Onfll 002 K ." 05-09-12 1002 K. Keenan Ped -Ix 1-5 7913 120509.15 iemenais _K, 05-09-,2 1002 K. Keenan Fed -Ex 1.1 7912 120509.16 Canrlina Beach WWP NC 05-09-12 1020 K. Keenan UPS 08. 7913 120509.17 Soath WWyWP NC 05-09-12 1020 K. Keanm UPS 5.8 7914 120509.18 Envio Chemists -MaeoToWWTP NC 05-09-12 1020 K. Kamm UPS 2.0 7915 120509.19 Buormawts -R ,WWTP NC 05-09-12 1020 K. Keenan UPS 2.0 7916 120509.20 Miaobac Enrrviamneontl -Roseboee WWIP N _05-09-32 1020 K aemmu UPS 2.0 7917 120509.21 M;7bue r -FJP Stvns -Ws WWIP NC 0.09-12 1020 K.Keeam UPS 2.o 7938 120509.22 MIuImblEi m -" -, 002 Environme -NC 05-09-12 1020 K. eLeKmm UPS 2.0 7918 120509.23 Siaob. aEnvironnsel-Trn.Minstai --Otell 003 2_05-09-12 1125 K. Keerm Dwh Courier 1.2 7919 120509.24 OWASA __KC 05-09-12 1300 J TVA Courier 1.6/1.7 7875 120509.25 TVA -So a NuclearPlant -101 TN 05-09-12 1300 J. Sumner TVA Courier 1.6 7875 120509.26 MTA -Se Ncbear Plant. Intake TN 0509-12 1522 L Sumer TVACouridr 2.2 7876 120509.27 IVA-Wa f Nuclearelarnt-Oatbl 1501 TN ....05-9-12 1522 J.Sumner TVA Courier 1.9 7876 120509.28 TVA -Wag BarNuclee Plant -Intake 101 o"T 05-09-12 1522 1. Sumner TVA Courier 2.5 7876 12050929 TVA -Watts BarN]cloe Plait- Ouln 113 05-09-12 1522 .Sfmter TACari 3.8 7876 120509.30 rVA-W tBwN,,,l learnPlat-Intelr 113 TNF 1 SOP 04 -Exhibit 04.2, revision 01-03-12 BIOMONITORING CHAIN OF CUSTODY RECORD Client: -TVA Environmental Testing Solution, Inc.I Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street.FedEx UPS Bus P.O. Number: N/A Asheville, NC Other (specif'y):_________ I-L -I Facility Sampled: Sequoyah NP 28801, General Comments:, 4-,.ieg,.( ,+A 04iý.f 06 0555 0'dp-fld4 , -+ coo.NPDES Number: TN0026450 Phone: 828-350-9364 _=.828-350-9368 Collected By: Dustin Bi Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6 0 C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. (DETS s Whole Effluent Toxicity Sample Receipt Log OSampl elmeperatureperformed using Sample Pam" iThermometer. SN 6338.0-9,Time Rcde-d Reed"A Smple ýPrjed Sample Sample name and dem4lptlf State Commana useelyad received byIV temperaure " ( ambCe 05-11-12 1014 K. Ke-an Fed -EX 0.7 7898 120511.01 CORR ERI -AAF M e Interatioenl NC.....05-11-12 1014 LKaman Fed-Ex 1.0 7IS 120511.02 W'P NC 05-11-12 1014 Kw Keean Fed- Ex 1.6 7900 120511.03 Duke Eae -Bedews Crac aStalon NC 05-11-12 1014 .Keenan Fed- ex 0.4 7901 120511.04 PrrssEneg -CapeFearS. NC 05-11-12 1014 XKeaman Fed-Ex 0.4 7901 120511.05 P ssEnery --CapeFFeS.K-U esrebvam wke NC 05-11-12 1014 3KLKaman ,.Fed-E 11 7902 120511.06 Ci oft wia-Dalls .WWP. NC " 05-11-12 1014 K. Keenert Fed -Ex 1.5 790 120511.07 United Water- Earield .AWTP NC 05-11-12 .1014 -I[ Kenan Fed- Ex 1.4 7904 120511.08 Duke ner -Mshall Soma ,Staion NC 05-11-12 1014 K. Keman Fed-Ex 1.2 7905 120511.09 AmnmomgReomme-Ralei CC NC,_,,_ _05-11-12 1014 K. Keenan Fed -aH 2.2 7906 120511.10 Bdl terpses -Sadett Aores MHP NC 0511-12 1014 KKeenwn d-Ex 1.S 7907 120511.11 UnritedWater-ScotIaed ek WWWP NC 05-11-12 1014 F Fed -Ex 0.9 7908 120511.12 Duke Energ -MoGuire NS -Oud M .5 NC 05-1-12 1014 F. Kamand-H , 1.0 7911 120511.13 alemnands NC 05-11-12 1014 K. Kew= UPS 0.8 7913 120511.14 SouthC WW, NC 051112 1014 K.Keenan UPS 0.8 207915_ 120511.15 MiNobacEnvirwum R ne1a-.ki n.m P NC 05-11-12 1014 .Keman UPS 0.8 7916 120511.16 MiarobaEnvironmental-RomboreWWW N 05-1-12 1014 K&eKama UPS 0.8 7917 120511.17 hMiebacE mn *X1PStevam-We , V'C ? NC , 0 5 -1 1 -1 2 1 1 2 3 K .K ee nm D a dh C ouPeS 0 .9 7 9 1 9 1 2 0 5 1 1 .1 9 O W A S A N C _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _05-11-12 1225 L. ST mw TVA Comier 0 .S,6 7875 120511.20 TVA -S Nuclear Plant -OutFl 101 TN 425-11-12 1123 K. Keeane D A Courer 2.1 7 375 120511 91 TA -iSA h N u ePl aed -I ntake IN_05-11-12 1432 L Sumner TVACouier 3.6 7376 120511.22 "VA-WatS BarqNuearPlant-ufaIi 101 N 05-11-12 1437 L Sumner TVA"Comier 4.5 7876 120511.23 TVA-WeatBarNlelPlmt -ntrete 101 "IN 05-11-12 1437 A Su TVA Couier 2.4 7876 120511.24 TVA -Watt BarNuolerePlanl -OuMli 113 T_05-11-12 2437 J. Smmer TVA Coerer 5.3 7876 120511.25 TVA -WaefsBer Nuclear -Intase 113 TN SOP 04"- Ehdibit 04.2, revision 01-03-t2 !131ý*" sET 1- MdrornnalT"stlng So~u~omInc Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Sequoyah Nuclear Plant NPDES#: TN0020168 Project #: :1 &-is County: Rhea Outfall: 101 Dilution preparat in ormation:Commen Dilution prep (%) 10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: .#" Test information: Organism age: AA -we- A ,a. 61'ý Randomizing template:

00 Date and times organisms

-t Incubator number and were born between: .shelf location: _ -" Organism source: ( .Artemia CHLM number: rI-cM b61.Drying information for weight determination: Transfer bowl information: pH = S.U. Date / Time in oven: !gj-1jL .IC0 Temperature = A- aC Initial oven temperature: 66 OL Average transfer volume: Date / Time out of oven: 0%-p.l %L, 01610 Final oven temperature: lob X._ _ _ _ _ _ _ _ _ _ _ Total drying time: Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 0 QSM- , 1.7t'- O&MC A t%40 M vasa A, .': 2 ... ... J l lO "c-t jh 40 .' 4b* 04 -lg a.Oo'1. -M IjjS-6.-L 06-0F*1 6 .9 2V 11 Ac il 12 IjCB l I 7.9 .0 ...~- ---l~bO 14 Control information: Acceptance crteria Summary of test endpoints: % Mortality: 0-. < 20% 7-day LCso .7=I .Average weight per initial larvae: 0101 NOEC &(.. q 7.Average weight per surviving larvae: 0. >0.25moarvae LOEC age.,I'...age 31 of 110 ChV IC 2 5> loah.SOP AT20 -Exhibit AT20.3, revision 06-01-Il1 ITPage 2 of 6 I Species: Pimephales Dromelas Client: TVA / Sequo'vah Nuclear Plant. Outfall 101. Non-treated Date: oG-oM- ti-Survival and Growth Data Day CONTROL 10.8% 1 21.6%AO B0 to D0 E0 F Go HO IO J0 K 0 to t. to to Io o to 101 1 to / o 11 'o ID 1_0 o A) to10 /0 /o 10 to 2 to Ito 10 (o (0 /0 10 /0 /0 /0 to/t_ 1 10 tO to ) to ( I 1 )0 10 /t) 0 It C)__ _ _ _ 11D 10 '10 10 C) .1o it) to 1010 1 1() it 0 6 tI to tj ito t0 to /0 (/0 /0 '1 '0 7 It /o to -10 o1 o 10o to /o L tO A -Pan weight (0ag0 Tray color code: L. t Analyst fA4 134j IL.% U4 14.A 'I.SS '40.o tZ..9D 14 .1 1o0, 14".h Date: oS.Ceo..I. B = Pan + Larvaj wehght (W)Analyst: " .,LJ Date: 4 lq 0e IU.* Val. .20 1.1a-C = Larvae weight (rag)- B --A Hand calculated. 4 4AA 1-11 .M l 1.16-Analyst:. _ .I I I Weight per initial number of larvae (rag)= Ir / [ngf']l Lnha r In Hand calculated.

j IA Analyst: , .-% 4 " ,cY 0*'a I', Average weight per Percent reduction 0 initial number of from control (%)101-larvae (ra)---I -, ~ 4. m I 0. .~S I-7 0.6164q~.. 17, h U -Comment codes: c = clear, d = dead, fg = fungus, k = killed, m -missing, sk = sick, sm = unusually small, 1g= unusually large, d&r = decanted and returned, w = wounded.Comments.A I_ age 32 of 110 SOP AT20 -Exhibit AT20.3, revision 06-01-11 Page 3 of 6 Ef~~~3TU~ilSUWI fC Species: Pimephales promelas Client: TVA / Seguonah Nuclear Plant, Ouffall 101, Non-tregted Date: 6._ _ _ Survival and Growth Data Day 43.2% 86.4% --100% -M N- 0 P Q -S T/13 10 (0 (0 (0 to t ) /0 ' /0 /0____/0 10 10 10 ) 10 Ito 1( 1( it) 0 Q 0 /o 2 16 /0' /0 10'/o O '/0 O /o /0 /0 /o0 3 1O IQ (0 '0 ti o cO to 10 to t0 10 4 A....) 1 ID 1t10 ,o o ,o 1,o ,0 10 1 0o ,o to 0__U_1() 10 0(10 01 / /0/0 /0 10 10 6 -,- ,o ,10o to to1,010 10 /01 010, A =Pan weight (mg) 1 Tray color codc:- Rut Analyst: 1+0. 14.3 "/. , 4 13. 1t, 29.2. t .-1l ¶.4 3Iq.qS' i,.Ls. 13..0 C = Larvae weight (rag) -B -A Hand calculated.'

10-142 to.1.- (.& 6-S 6Ai R 161 %,4% 61 b-C /Initial number of larvae Hand calculated. 4'Analyst: d0.q-,"!" !" " O EAve~raige weight per Percent reduction Initial numberof from control 176 0.' S.t11. '12.. o11.47 1 .a.larvae m_____ __-Comment codes: c = clear, d = dead, fg = fumgus, k = killed, m = missing, sk = sick, sm =unusually small, Ig = unusually large, d&r = decanted and returned, w wounded.g commentsn age 33 of 110 SOP AT20 -Exhibit AT'20.3, revision 06&01 -I! EI~rnn~15fgab~I T ,c Page 4 of 6 Species: Pimenhates promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101. Non-treated Date: oC.Ot- VL.-I I I I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.34 of 110 SOP AT20 -Exhibit AT20.3, revision 06-01-11 (iii (Dh V Environmental Testing Solutions, Inc.TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 08-15,2012 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project aumberi 7375 Not ff COM' As- mema, Laboratory0 0mnmh(%) Iilinte l n -ed etaaeh bngm A -Pz Wd 0 Ii (iq) It-PFi4 las,,.tlae Aa.-d (mg) Bmiit M~mwl.hsI CeeeaI .aalr tI Wcahtlldld asAs, 96e.a.n mf aitl 11td C ebeatidd eef t freIs I.- wdgti (ma -A-B nharorla.(q S-hlrh 3 aser or o ,h'r.'I a5sse(a) (M1 *."b ara._ v-arin ,d-ak catre (%)A 10 10 13.69 20.27 6.58 0.658 0.658 B 10 10 12.78 19.64 6.86 0.686 0.707 7.2 100.0 0.707 7.2 Net applicable _ontre__ 10 10 14.17 21.24 7.07 0.707 0,7070 D 10 10 13.67 21.45 7.78 0.778 0.778 0 10 to 14.65 20.89 6,24 0.674 0.624 I ___"__10 10 14.77 21.12 6.42 0.642 10.8% 10 13.6 12.71 6.4 0.64 .8 0.648 100.0 0.655 4.8 7.5 G to 10 14.07 21.06 6.99 0.699 1H 10 10 1339 19.86 6.47 0.647 0.647 1 10 10 12,86 19.15 6.29 0.629 0.629 21.6% 1 10 .10 ,M4AD 21.77 7.17 0770.681 6.4 .7791.5 0.664 9,A 6.1]K 10 9 14,08 20.52 5.94 0.EE6 0 I L 10 10 14.11 2127 7.16 1 0.716 0.716 1 M 10 to 14.30 21.09 6.78 0.678 0.6"78 43.2% --N 10 to 14.159 21.43 6.74 0.674 0.667 2.5 0.674 100.0 0.667 M 5 5.7 0 10 to 14.77 21.19 6.42 0.6642 0.642 P 10 10 12.43 19.22 6,34 0.674 0.674 L Q 10 10 13.72 20.32 6.60 0.660 0.660 86&4A IR 10 10 13.67 19.71 6.04 0.6D4 0.642 4.0 0.604 100.0 0,64 &.0 9.2 P t 100 13.07 19.64 6.57 0.657 0.67 T 10 10 132241 19.72 6.48 0.648 0.648 U 10 10 13.23 19.44 6.21 0.621 0.621 100% V 10 10 14.98 21,32 6.34 0.634 0.631 6.3 U.34 100.6 0.631 6.3 10.9 w 10 .. 10 1.4.65 20.51 5.96 0.596 l 0.596 X 10 10 13.58 20.40 6.82 D,682 0,82-Y 10 10 14.01 21.35 7.34 0.734 0,134 z 10 10 13.43 1 19.T77 6,34 0.634 06463064lOO 06463L 100% Italke AA 10 10 13.'70 1 20.63 6.93 069 0,66.93t .6463L.BB 10 10 ,13.51 1 _2D.67 I 7A16 0.716 1 0.716 1 OuItfoU101 Thianelts IWSD value: PMSD-h Ta~t" Donaetl's IWSD value;PMSWD 0.0697 9.9 9.2 MSD -Minimum Significant ifference PUSD = Percent Minimum Significant Diffeence PMSD is a nmaure of te precision. The PMSID is the minimnu percent diffrenoe between the control aid beatment that cam be declared statimically significant in a whole effluent toodiity test.Lower PIMSD bound determined by USEPA (l0th percentile) -12%.L~pper PMSD bound determined by USEPA (90th percenfile) -30%.Lowe and upper PMSD bounds were deterrnned from the I th and 90th percentile, respectively, of PMSD datam fio EPA's WET lnterlebortory Variability Study (USEPA, 2001 a; USEPA, 2001 b).USEPA. 2001 a, 2001b. Final Report: lnrtaburata Variability Study of EPA Short-term Chronic and Acute Whole Efluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821 -B-01-005. US Environmental Protection Agency, Cincinnati, OH.File: sq,101. 50812dala.dax Enterd by:. J. Sutner Reviewed by,. TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 08-15, 2012 Statistical Analyses el 0* inA-Uwm.Larval Fish Growth and Survival Tret-7 Day Growth Start Date: 5/8/2012 Test 1D: PpFRCR Sample ID: TVAI SQN, Outfall 101 End Date: 5/16/2012 Lab ID: ETS-Envlr. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.8680 0.6860 0.7070 0.7780 10.8 0.6240 0.8480 0.6690 0.8470 21.6 0.6290 0.7170 0.5940 0.7160 43.2 0.6780 0.6740 0.6420 0.8740 86.4 0.6600 0.0040 0.6570 0.6480 100 0.6210 0.6340 0.5860 0.6820 Intake 0.7340 0.6340 0.6930 0.7160 Transform: Untransformed 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7073 1.0000 0.7073 0.6680 0.7780 7.248 4 0.7073 1.0000 10.8 0.6545 0.9254 0.6545 0.6240 0.8990 4.839 4 1.823 2.410 0.0697 0.6818 0.9358 21.6 0.6640 0.9388 0.6640 0.6840 0.7170 9.380 4 1.406 2.410 0.0697 0.6618 0.9358 43.2 0.6670 0.9431 0.6670 0.5420 0.6780 2.515 4 1.391 2.410 0.0697 0.6818 0.9358 88.4 0.8423 0.9081 0.6423 0.8040 0.6800 4.049 4 2.247 2.410 0.0897 0.8423 0.9081*100 0.6308 0.8918 0.6308 0.6860 0.6820 6.298 4 2.644 2.410 0.0697 0.6308 0.8918 Intake 0.6943 0.9816 0.6943 0.6340 0.7340 8.270 4 Auxiliary Tests Statistic Critical Skew Kurt Shaplro-Wllks Test Indicates normal distribution (p > 0.01) 0.97188 0.884 0,17128 -0.4666 Bartlett's Test Indicates equal varlances (p = 0.39) 5.23389 15.0883 Hypothesis Test (1-tall, 0.08) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 86.4 100 92.9516 1.15741 0.06973 0.09869 0.00279 0.00167 0.19306 6,18 Treatments vy D-Control Linear Interpolation (200 Resemples) Point % SD 95% CL(Exp) Skew C105" 8.409 ICIO 93.170 IC15 >100 1.0 IC20 >100 IC25 :-IGO 0.9, IC40 >100 o.a IC50 >100 0.7* indicates IC esUmate less than the lowest concentration I0.6: 0.5'0.4'0.3: 0.2: 0.0 0 50 100 160 Dose %Dose-Response Plot 0.9 0.8 0.7 08: 0.5 OA , 0.3 0.2 0.1!... ~ I1-tall, 0.05 IeVel I I of algnriffance ~1 S S 6-N.- 0 a Page 36 of 110 File: sqal10lOSO8l2detauxli Enterd by: 1 9zie Reviewed by: _ TVA / Sequoyah Nuclear Plant, Intake Non-treated May 08-15, 2012 6 OETS a Grw~ronmrftaiTmd"nuS~lutiafs. In Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/8/2012 Test ID: PpFRCR Sample ID: TVA / SQN, Intake End Date: 5/15/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.6580 0,6860 0.7070 0.7780 10.8 0.6240 0.6480 0.6990 0.6470 21.6 0.6290 0.7170 0.5940 0.7160 43.2 0.6780 0.6740 0.6420.. 0.6740 86.4 0.6600 0.6040 0.6570 0.6480 100 0.6210 0.6340 0.5860 0.6820 Intake 0.7340 0.6340 0.6930 0.7160 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.7073 1.0000 0.7073 0.6580 0.7780 7.248 4 10.8 0.6545 0.9254 0.6545 0.6240 0.6990 4.839 4 21.6 0.6640 0.9388 0.6640 0.5940 0.7170 9.380 4 43.2 0.6670 0.9431 0.6670 0.6420 0.6780 2.515 4 86.4 0.6423 0.9081 0.6423 0.6040 0.6600 4.049 4 100 0.6308 0.8918 0.6308 0.5860 0.6820 6.298 4 Intake 0.6943 0.9816 0.6943 0.6340 0.7340 6.270 4 0.387 1.943 0.0653 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97462 0.749 0.1774 -0.6068 F-Test indicates equal variances (p = 0.79) 1.38665 47.4683 Hypothesis Test (1-tall, 0.06) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.06534 0.09238 0.00034 0.00226 0.71238 1, 6 Treatments vs D-Control Dose-Response Plot U.w .0.8 0.7 0.6 0.5 0.4' 0.3 0.2 0.1 1-tail, 0.05 level of significance ll6 c I a tage 37 of 110 File: sqnl01.050812dataxlsx Entered by: J. Sumner Reviewed ,by: F~jrstunga~kSffI slbusa] In Page 5 of 6 Species: Pimevhales promenas Client: TVA / Seauoyah Nuclear Plant. Outfall 101. Non-treated Daily Chemistr: Date: CA- of" '..I Dav I Analyst I Concentration IParameter PH (S.U.)Conductivity (umhos/cm) CONTROL Non-treated S. ....... .1-Alkalinity (mLY CaCOi/L)* -..Hardness (mg CaCO 3 IL)Temperature (QC)U ---p DH (S.U.)~-~-~---~ U DO (ma/pjL)4 10.8%Conductivity (Umhos/cm) Temverature (0*B -m ~. --pH (S.U.)DO (m/LL)21.6%Conductivity q (P os/cI Temnerature (0 C)-A-A A PH (s.u.)43.2% Conductivity (gtmhos/cm) Temperature (TC) "lk, PH (s.U.) _4 t 86.4% Conductivity (Enhos/crn) Temperature (TC)PH (S.U.) , Conductivity (Mmnhos/cm) 100%. Alkalinity 100% (Mg CaCO/L)Hardness TR chlorine (mg/L) C

I_Temperature (0 Q 150 I, 11 oH fS.U.)I 100%Intake Conductivity (pmhostent)

Alkalinity (Mg Calrie(/L) Hardness TR chlorine (mg/L)-age 38 of 110 Im Temperature (0 C)27.L a -Initial Final Initial Final SOP AT20 -Exhibit AT20.3, revision 06-01-11 ýaimietIEThtrgSJmotI Page 6 of 6 Species: Phnephda prog4as Client: TVA / SeIuovah Nuclear Plant. Outfall 101. Non-treated Date: 1".-I Day I Analyst 11 t fA I .LA.. I Concen- Parameter I tration U 1 s~'I I PH (S.U.) V,%I DO (me/L)CONTROL Non-treated Conductivity (E!lRhosym Alkalinity (Mg CaCO3/L)Hardness (Mg CRC03/L)114.6 21014 5M 65 8-1_2A. R 11 f.l.%4 #Temnerature (0 C0-4 ll Rv w i V In pH (S.U.)II -~ (n 11'4+5 1.13 DO (mg/L)10.8%Conductivity (Ounhos/cm) 1?1, L14 IL1411 Temperature (0 C)16-.0 i~M2L2L33L JJJZ~.Z'4., 2q I L.L pH (S.U.)DO (mgIL 21.6% Conductivity (T-mhos/cm) -~Temperature (QC_pH (S.U.)DO (in1L 43.2% Conductivity phos/cm 1.________ I Temperature (*CQ 1 312-1A.. A Z00 W+Wt.AW-Ln- I 14. &w 0.0 V1 55 oH (S.U.)5? to 86.4%DO (mg/L) 1 Conductivity, (Amhos; 117 AIS Temperature (MC)__ __ __ I_ I -b ....pH (S.U.)i DO (mg/Ly imam 'J.*ERT~a EERLIW2 MOINWFUM MU 100%'Conductivity GA~nhwslcm). Alkalinity (g COCOA/)Hardness (Mg CaCO3/L)TR Chlorine (mrgL)Temperature (0 C)I i pH (S.U.)DO (mg(L)I 100%Intake Conductivity (gmhos/cin) Alkalinity Hardness (Mg CaCO 3/L)TR chlorine (mg/L)m I__ 7ge 39 of 110=..-Iaur (0 C I_ý a I -- 1' " qI %.-&.Initial Final Initial Final SOP AT20 -Exhibit AT20.3, revision 06-01-11 co E0 Environmental TetngSouinIc TVA / Sequoyah Nuclear Plant, Outfall 101 -Non-treated May 08-15,2012 Pimephalespromdas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Projeet number. 7875 Concentration Parameter Da 0V Da 3 Initial Final Initial Final Initial Final initial Final Initial Final Initial Final Iaitial Flnal'H (SU) 7.85 790 7.92 7.79 7.95 7.85 7,98 7.70 .8.02 7.67 7.97 7.62 8.06 7.49 DO (mraL) 7.6 8.0 7.8 75 7.7 7.7 7.4 7.2 7.8 6.9 7.6 7.0 7.6 6.4 Conductivity boa/em) ...... 3 310 303 294 309:h 309 3 Control Alkalinity (mg/L 63 63 63 63 63 62 Hardness (mngL CaC(O) 88 88 89 87 89. 1 89 Temperature (0 C) 24.9 24.9 24,8 24.6 24.8 24.7 24.8124 24.8 25.0 24.8 pH (SU) 7 7.86 7.96 7.79 7.90 7.85 8.07 7.59 7.95 7.60 7.94 762 810 7 DO( L) 79 7.9 7. 7.4 7.8 7.7 7.7 7.1 8.0 6.8 80 70 76 10.8% Conductivity (ronboslem) 306 297 292 294 : _____2&3 291 Temperature (C) 249 24.9 25.0 24.7 24.8 24.6 24.9 24.5 24.8 24.7 248 248 248 24.7 pH (SU) 789 7.86 7.98 7.78 7.92 7.79 8.10 7.62 7-92 7.56 793 764 810 749 DO_(muIL) 79 7.9 7.8 7.6 7.8 7.7 7.7 6.8 .0 6.5 80 69 76 66 2. Conductivity (puhos/em) 29 28__)4 -2 280 8 '7.6.2-8.. lTemperature CC) 249 24.6 25.0 24.7 24.8 24.5 24.9 24.8 24.8 24.9 248 248 248 247 PH (S 7.89 790 7.99 782 7.91 785 8.09 7.73 786 756 787 763 811 747 43.2% DO(0a11) 79 80 7. 76 789 7.7 7.7 6.8 8.0 66 80 70 76 65 Conductilvity (Iumhos/em) 5 " 259 257 258 _ %153 O-O _M,_ 263 263 ,empertureCC) 24.9 24.6 25.0 24.8 248 248 24.9 24.6 248 246 248 251 248 247 PH ( 7.91 790 8.01 782 7.88 787 8.10 7.69 781 763 783 765 812 746 DO 7.9 8)0 7. 9 7.6 7.9 7.7 7.7 7.0 80 69 80 69 76 65 86A% Conductivity (jmuho/cm) 2 210 211 212 6 ARM= '? 21521 Tem2rature CC) 25.0 246 250 246 248 24. 24.9 24.6 24.9 248 248 250 24.8 pI (SU) 7.92 790 02 786 7.89 792 8.05 7.75 7.76 7.61 778 7.65 .16 7 DO (Mg/L) 7.9 0 7.9 76 7-9 7 7.7 6.9 8.0 6.9 80 69 7.66 Conductivity (puhosemt) 197 193 197 196 W 41i 1 100% AlkUalinity (mg/L CaCO 1) 79 ..... 81 79 Hardness (mg/L CaCO 3)- 81 Total Resdual Chiorlne (ragL) <0.10 <010 MAIM ... .0.10 1 Temperature CC) 25.0 248 25.0 246 25.0 247 24.9 24-7 25.0 24.9 24.8 25 25.0 24.5 PH (SU) 7.95 793 8.04 784 8.04 790 8.12 7.75 8.06 7.65 802 7.6 8.16 7.53 DO (mreL) 7.9 7.9 8.1 7.5 8.1 8.0 7.7 7.0 8.0 6.7 8.1 7. 7.7 6.7 Conductivity (pmhos/em) 195 190 192 194 182 9 190 100% Intake Alkalinity (mg/L 79 79 Hardness (mg1L CaCO 3) 79 81 3 79 Total Residual Chlorine (minl) <0. I <0.10 -.<0.10 Ten,,,-tre C) 25.1 248 25 24.7 25.0, 24.7 24.7 25.1 24.9 25.11 24.9 -4.7 File: sqn101_050812chem.xls Entered by: C. Johnson Reviewed by: OFETS Page 1 of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)Species: Cerlodaphnia dubia Client: Tennessee Valley Authority Facility: Seauovah Nuclear Plant NPDES #: TN0026450 Project #: 1815 'County: Hamilton Outfall: 10 i Dilution preparation information: Comments: Dilution prep (%) 10.8 21.6 432 1 86A4 100 Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0 Total volume (mL) 2500 2500 2500 .2500 2500 I I I Daily renewal information: I I Day Date Test Initiation and feeding, MHSW Sample numbers used Analyst renewal and feeding, or batch used Outfall 101 Intake--~~~~termination time _______0 Ao, .oe ___. _ .A 1 1 O1-, 0 CS M-eS QAM.Q', i-Loa - I 7" r2 S&d KIM1 AL6511 cis__ ___- A I_____ i O .L k I 6 n~. .14.aL n tLit-.MAIZE V6. 70%18La&1 7.24 7-4-A Control information: Summary of test endpoints: __ Control-I Control-2 Acceptance criteria% of Male Adults: 07. :07 s20% 7-day LCso I MI.% Adults having 3' Broods: 1., O80% NOEC 1013%% Mortality: .. : 20% LOEC I1007.Mean Offspring/Female: 31.1 3.4. Z 15.0oiTspringfemaIe, ChV (Cal%cv: to-al7. .7. <40.0% IC 2 5>Ioo (Y age 41 of 110 SOP AT 11 -Exhibit ATI 1.2, revision 06-01-11 I I TTS Page 2 of 7 Species: Ceriodaphnia dubia Client: TVA j Seguovah Nuclear Plant, Outfali 101 CONTROL- -Surv, Date: OS* "II.-hvaI and Reproduction Data I... Replicate number Da 4 516 7 18 9 10 I Young produced C) ° °D Adult mortality. T 213 4 , 6 -9 10 IL %, I ""- t- ' 9 f 2 Young produced C) 1 0 ..d 0 , ...Q. 0 .Adult mortality 1 L. L ._L L.. L 3 Young 0" "E6 Te( 'L'll, ". i Adult mortality

t. I II-- L.I .L- L- ! -j 5Young produced JLL I -p-- -.- + -- 0 1 i " Adult mortaliIty ____ ,-t _ _- i,-6 Young produced J .InTl..I.~.131]

Adult mortaity ----' '-_ -_-1 -1L...7 Young produced t 1 T% PA P4~ t ~Toa young produced -6 37 .3 3513 -1 'e iFinal Adult Morta-lity k..- %. %.- I. ~%.-. C7- C 1 %.-.X for 3- Broods I:: X- >C-- I X- 2E Z-" t Motw: Adult mortality (L -live, D -dead)' SB slit brood (single brood split between two days), CO -canym over (omrg carried over with adut during trmasfir Concen&raon: Mcan Of ring/Fmnale: I CONC: 10.8% Survival and Reproduction Data__ .. .. Replicate number Day 1 2 3 41 , 6 7 8 9 10 SYn produced I iAdult mortal~ity ] L- ., .. L- L. .L [=2 Young produced I _ p I 0 "0 0 0'1 I 0 4 Adult mortality It L .I 1 .L L. L-3 Young produced -0 0-d -..., = 1' = I I =_-Adultmortality t' ._ + -Q -i tC '" 3 Young produced I -Adult mortalityJ LX -. _ U. L LJL- I 4 Young produced ' --S 111 -=Adult mortality-c- -1 UI .5 Young produced LEJ. 2 t i t1j 1. ' iL Adult mortality ~J[~ U I Z 6 Young produced %S.;~Total young produced IL 1 2 2z. as %ss s Final Adult Mortality Z t- C .%-. = I z --1 Note: Adult mortality (L -live, D = dead), SB -split brood (single brood spln betweem two days), CO -ean ovr (offca r arrnied over writh adult duins t ." Concentration: I* ge 42 of 110% Mort~ality: Mean Offspring/Fernale: z-A%'Reduction from Control-I: -0.1 7 K SOP ATII -Exhibit AT 11.2, revision 06-01-11 o'TTS Page 3 of 7 Species: Cerlodayhnla duba Client: TVA/Setwovah Nuclear Plan. Outfall 101 CONC: 21.6% SurM Date: n.oI'l2.ival and Reproduction Data p a I I I 1_Replifcate number .Day 1 2 3 5 6 7 1 9 ,o 1 Young produced .) 0 c). M E = =Adult mortality "1_. L.. L t, ." i.-- .C2 L_ "...- t-- -" Adult mortality t U..] _... LL 2 Young produced 0 0 0 0 I _ _ -_ _ -_ _ -aa 3 Young produced 0 U 0 " --T L -Q-Adult mortality = U. U_

U L 4 Young produce d -'4 1.. S '2L L Adult mortality A, -- .._ , 5 __Young produced I~V.~ j A ~ IL Z S I ý2.Adult mortal ity ..L .. U ... ..6 Young produced L.~I~ ..~ ~~1~IQ.~ -- Q.Adult mortalityJ\IL L L. 4 t' -' .7 Young produced I %L TI IS II S A IS, Is 1A- 11 Total young produced Is 671 i .24t. as .' 32 3S a7. S 3 Final Adult Mortality 10 L- , ~T -'Note Adult mortality (L = live, 0 = dead) SB = Split brood (fingle brood spll between two deYn). CO-C ow (ofle ing carried over with adult during trnaser).Concentaion.-

% Mortality: Mean Offspring/Female: % Reduction from Control-1: -S.CONC: 43.2% Survival and Reproduction Data Replica number Day 1 2 3 4 5 6 7 9 10 1 Young produced n -b -0 o -__ o'~ o) occc Adult mortality tj L__ .LU. _ L_ __ (_ ~ -t- t.2 Young produced 1 Adult mortality ] J --I. I~7 -3 Young produced C" ._ L.At I -- a_. .0-. iQ fl 0 SAdult mortality. L U1 _. ..L......4 -Young produced q' qis %A 3 I:__ Adul~t mortality ~ U .~ iUU Y Young produced t4 q 4 l i '4 -i [Young produced -= 0_ UT.L_ x.IAdult miortality L__C I t.-L U.7 1 Young produced %1... I ..j... Q...Toa young produc = ____ __ ed -I- --ISayunpoucd3 335 31 33 S f .3 S 51 31 3 33 Final Adult Mortality

= -1 t- I -I -K: I ._ \_I I AWon: Adult mortality (L = live, D = dead), S3 = 81lit brood (sinOe brood between two days), CO m cr over -oapfm canted over atu r Concentrallon:

% Mortality: 07.Mean Offspring/Female: .1% Reduction from Control-I -. -,. ,, SOP AT!I1 -Exhibit AT112, revision 06-01-11 I ( P ge 43 of 110 I I I I I Ia Page 4 of 7 Species: Ceriodaphnia duba Client: TVA / Seauovah Nuclear Plant Outfall 101 coNc: 86.4% Surn Date: fl(.8'll.Ival and Reproduction Data 1 Replicate number Day 1 2 314 S 6 7 8 9 10 I Young produced QO Q J CO Adult mortality-L. j L _ I...2 Young produced C c7. ..p -..Adult mortality _-. -. .Lj L.... ._ L L.Adult mortality 0 -1 0 44 You--ngprod-u L L.e-d I Young produced 0 Adult mortality [ l._J i. t.I I Adult mortality~on proJ need ti... L0% _ __6 Young produced at~76 Yo] U 1 0' 1 Total young produced S4 3-L 3 , 31 3S -34 %%A 3 -IS Final Adult Mortality -7 %_ .. ' ..? , .Not: Adult motaliy. (L live, D -dead). SB- psi brood (slagl brood spit between two daya). CO -over =, aned over with adult sing transkel Concentration: % Mortality: Mean Offspring/Female: % Reduction from Control-h: ". 2.7,, CONC: 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced*p p -- --AdultImoity 2 Young produced Young produced ." ...X ...cL Adult morta lty l -L. ' ..L .L L ] C.---S Young produed -Adult mortality I L C. -- C U L.- L ,- r-5 Young produced dmb is =- _ T Adult mortal"t 5 Young produced. 6 1..'L7IYoung produced k__'R i Total young produced 6 3S 3 R 3S f 6.1 II -Final Adult Mortality I ._ I._.. --7 .K.. t I I I I I I Noe.: Adult mortality (L -loe. D -dead), SB -Spit breed (single od sprt between two days), C -Crover (ofpain carried over with adult durin transfer). Concentradon: % Mortality: Mean 1 fMxpringtemale: 0% Reducton from Control- 1: -1 SOP ATI I -Exhibit ATI Z.2, revision 06-0t-I11* ge 44 of 110 9:FT!;Page 5 of 7 Species: Cerlodaiaknia dubW4 Client: TVA / Seauovah Nuclear Plant, Outfall 101 CONTROL-2 Date: o6-01. 2.., 1val and Reproduction Data V I I I I Replicate number Day 1 2 3 4 5 6 7 8 9 10 Day ___ _ 2 4. 6 "__I Young produced Q p -H-c ..&- I Adult mortality I J _ .--, ,*, L.. --___2 Young produced O 0 i J O O. 1 0 .j 1 Adult mortality C. " Z U--- L.. ..- L L- .3 Young produced 4 )I*..00 Adult mortality '- -tJI '- '.- -- -_4 Young produced __I %4 -, h +H ? __ -___________.___,_ I .L I L ,, i , I 5 Young produced ),-Il %t l i U 1. ] i Adult mortality -_ jI -' f _6 {Young produced6 01 L-c,,e, o,-Adult mortality %- Me. n i ga- I %- -'7 Youngproduced It I*5 i l TD 11b1 5I % II t Total young produced 30. 33 3. 0I3 21 -3. L 31 3123.(Final Adult Mortality fIJ \ -C C .V- I %. I- 'ý-tX for 3 Broods I -- :.&-M.. .r I .. .M 3ue: Adult mortality (L = Ilve, D dead), SB sot brood (uingle brood spit beteen two daysl 00 -carry ow (omprias carded over w -it adult during trae LConcenit rdn:% Mortality: Mean Offs ring/eniale: l cowC: 100% Intake Survival and Reproduction Data I ______ ______Replicate number ___Da dul 1otlt 2 3 4 16 17 8 9 101 1 IYoung produced Q e) 6 1C n ~ Ql~~Adult mortality L.. ._ L._3 Young produced _ _ C) I 0CL C Adult mortality U L_4 Young produced % S Li -,. , I. .'4 Adult mortality L ....... .. ... 114 -5 Young produced I 4jcr il 2.V Adult mortality -U '..' .-U U-6 Young produzced I 010 AS .L.IAdult mortality U --~ T U ..L 7 [Young produced -6 L4 -f IA I -Total youngproduced .31 a t. 31 31 31 J- 31 a! 31 1 Fia Adl ,r1:=I I I I I I I I I Adult mourtality kt.=nW., D~e~ = aWpS Iwigorood (Waga bomod 3PWbeween cm e, otze Menover _. adou r, I ge 45 of 110 Concentration: % Mortality: Mean Offpring/Female: 3.% Reduction from Control-2: _ -"4,ZJ SOP ATI 1 -Exhibit ATI 1.2, revision 06-0.1 -11 ~0 0 Emdnmn Trg $ol- uem In.o Control-1 TVA I Sequoyah Nuclear Plant, Outfall 101 -Non-treated May 08-15, 2012 Verification of Ceriodaphnia Reproduction Totals')a Replicate number Dayeibe .Total Dy 1 2 3(4= 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0_ 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 6 4 5 5 4 5 46 5 12 13 10 12 14 12 13 12 13 10 121 6 0 0 0 0 0 0 0 0 0 0 0 7 17 15 15 15 16 14 15 17 14 14 152 Total 33 32 30 31 36 30 33 34 31 29 31I n ~ e~t ubrTotal33 3 30 3 31 3 28 3 35 3 32 1 2 3 4 5 6 7 8 9 10 h 0 0 0 0 0 0R0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 1 0 4 4 4 6 5 53 4 6 5 5 45 5 12 13 12 12 13 12 10 12 13 12 12_1 6 0 0 0 0 0 0 0 0 0 0 0 7 17 16 13 16 15 16 14 17 17 15 156 Total 36 32 30 33 31 32 28 35 35 32 323 21.6%DoReplicate number Total D y 1 2 3 4 5 6 1 / 7 8 9 110-0 0 0 0- 0 0 0 0 0 0 0 2 0- 0 0 0 9 0 350 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 r4 4 6 5 5 4 5 5 4 5 47 5 13 12 14 .13 11 13 12 12 15 12 127 6 0 0 0 0 i0 0 0 0 0 0 0 7 19 6 -16 15A 15 115 118 1 16 161 Total 36 2 '36 33 131 132 135 32 35 33 335 43.2%DyReplicate number -Total Dy 1 2 3 4 5 6 7 S 9 10 1 0- 0 0 0 0 0 0 0 0 0 0_2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 _0L 0 _L 0 0 0 D 0 0 4 4- 4 4 6 -4 4 6 5 5 5 47 5 14 12 14 14 12 12 It 13 12 13 127 6 0 0 0 0 0 1 0 0 0 0 0 7 FT 15 15 49. 17 114 181 19 1 15 165 Total 135 31J A3 19 33 130 135 137 133 133 3391 86.4%Repslicate numberToa Day 1 2 3 0 4 6 0 7 0 8 0 To1a0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 Tt 5 5 4 5 5 6 34 4 3 6 48 5 12 13 13 15 13 13 11 13 12 12 127 6 0 0] 0 0 0 0 0 0 0 0 0 7 17 18 15 12 13 15 13 12 17 17 170 Total 34 36 323 30 31 35 34 341 3312 30 100%a Reicate number Total 3 4 0 5 6 0 7 1 8 0 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 _0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 6 5 4 5 5 6 4 5 50 5 13 12 13 12 13 15 13 12 12 14 131 6 0 0 0 0 0 0 0 0 0 0 0 7 18 19 16 1 120 18 15 18 17 17 177 Total 33 3 5 29 32 3713 32 31 33 13 323 Control-2 1 2 13 4 5 6 7 8 19 110 II 1 0 10 10 0 0 0 0 0 0 2 0 0 10 0 0 0 0 0 0 10 0 3 0 0 0 0 0 0 0 0 0 10 0 4 4 6 5_ 4 4 5 4 4 4 3 43 5 10 13 12 12 13 11 11 11 11 10 114 6 0 0 0 0 0., 0 0 0 0 0 1 0 1 7 16 14 16 114. 174 13 1 6 1 149 Total 30 33 33 130 1 9 32 31 31 26 1 3-6 100% Intake Day ~~Re pdkcte numberToa 1a 1 2 3 .1 4 5 1 6 7 8 9 110 Toa I 0 1 0 0 0 0 0 0 0 0 0 2- 0 0 0 0 0 0 0 0 010 0 3 0 0 0 0 -0 0 0 0 0 0 0 4 4 4 5 4 5 6 6 6 4 5 49 5 113 14 10 14 13 12 13 10 12 11 122 6 0 0 0 0 0 0 0 0 0 0 .0 7 16 17 14 14 113 18 13 15 17 15 T 152 1 I-Tota 33 13=3S 2-9 3 31 r6 2 31 3-3 31 1 323 File,: sqW11_O50812dataJdsx Entere by- J. Sagner Reviewed by: O E4n Environmental Testing Solutions, Inc.TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 08-15,2012 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0.Quaflity Control Verification of Data Entry, Calculations, and Statistical Analyses Project number'.7875 Concentration Replicate number I Survival Average reproduetlon C ue of Pem reducim from (%) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) Vaiimon (% ontol (%)Comtrol- 1 33 32 30 31 36 30 33 34 31 29 100 31.9 6.7 Not applicable 10.8% 33 33 30 33 31 32 28 35 35 32 100 32.2 6.7 0.9 21.6% 36 32 36 33 31 32 35 32 35 33 100 33.5 5.5 &0 43.2% 35 31 33 39 33 30 35 37 33 33 100 33.9 7.9 -6.3 86.4% 34 36 32 35 37 35 34 34 33 35 100 34.5 4.2 48.2 100% 37 36 35 35 37 38 33 36 35 36 100 35.8 3.9 -12.2 Control -2 30 33 33 30 31 29 32 31 31 26 100 30.6 6.8 Not applicable 100% Intake 33 35 29 32 31 36 32 31 33 31 100 32.3 6.4 -5.6 Outfid 101.Dunnett's MSD value: PMSMh Intake.Dununets MSD value: PMSD: 2.036 6.4 1.599 5.2 -MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.Lower PMSD bound determined by USEPA (100 percentile) = 13%Upper PMSD bound determined by USEPA (90'h percentile) -47%Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratoy Variability Study (USEPA, 2001a; USEPA, 2001b).USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OK USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01.004 and EPA-82]-B-01-005. US Environmental Protection Agency, Cincinnati, OH.File: sqn101._050812data.xlsx Table populated from associated 'Verification of Cerfodaphnia Reproduction Totals" spreadsheet. Spreadsheet entered by: J. Su ner Reviewed by: TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 08-15, 2012 Statistical Analyses*** inu~.o r~rn'n L~Lic2Y Cerlodaphnia Survival and Repmduction Test-Reproduction Start Date: 5/8/2012 Teat ID: CdFRCR Sample ID: TVA I SON 101 End Date: 5/151/2012 Lab ID: ETS-EnvIr. Testing S01. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2012 Protocol: FWCHR-EPA-821-R.02-013 Test Species: CD-Cenodaphnie duble Comments: Conc-% 1 2 3 4 8 6 7 8 9 10 Control-I 33.000 32.000 30.000 31.000 36.000 30.000 33.000 34.000 31.000 29.000 Control-2 30.000 33.000 33.000 30.000 31.000 29.000 32.000 31.000 31.000 28.000 10.5 33.000 33.000 30.000 33.000 31.000 32.000 28.000 35.000 35.000 32.000 21.6 36.000 32.000 36.000 33.000 31.000 32.000 35.000 32.000 35.000 33.000 43.2 35.000 31.000 33.000 39.000 33.000 30.000 35.000 37.000 33.000 33.000 86.4 34.000 36.000 32.000 36.000 37.000 35.000 34.000 34.000 33.000 35.000 10 37.000 36.000 35.000 35.000 37.000 38.000 33.000 36.000 35.000 36.000 Intake 33.000 35.000 29.000 32.000 31.000 38.000 32.000 31.000 33.000 31.000 Transform: Untransformed I-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Ciltical MSD Mean N-Mean Control-1 31.900 1.0425 31.900 29.000 36.000 6.683 10 33.833 1.0000 Control-2 30.600 1.0000 30.600 28.000 33.000 6.750 10 10.8 32.200 1.0523 32.200 28.000 35.000 6.877 10 -0.337 2.287 2.03a 33.833 1.0000 21.6 33.500 1.0948 33.500 31.000 38.000 6.495 10 -1.797 2.287 2.036 33.633 1.0000 43.2 33.900 1.1078 33.900 30.000 39.000 7.921 10 -2.246 2.287 2.036 338633 1.0000 86.4 34.600 1.1275 34.500 32.000 37.000 4.158 10 -2.920 2.287 2.036 33.633 1.0000 100 35.800 1.1699 36.800 33.000 38.000 3.906 10 -4.381 2.287 2.036 33.833 1.0000 Intake 32.300 1.0558 32.300 29.000 38.000 8.370 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test Indicates normal dlsalbution (p > 0.01) 0.88163 1.036 0.20051 0.0673 Bartletts Test Indicates equal variances (p -0.37) 5.43364 15.0863 The control means are not significantly different (p , 0.18) 1.38493 2.10092 Hypothiesls Test (1-tall. 0.05) NOEC LOEC ChV TU MSDU MSDp MSB MSE F-Prob df Dunnetra Test 100 >100 1 2.03677 0.06382 21.1887 3.98296 4.SE-04 65,64 Treatments vs Control-i Linear Interpolation (200 Resemples) Point % SD 95% CL Skew IC05 >100 IClO '100 IC18 ->100 1.0.IC20 >100 0.9 IC26 >100 0.8 IC40 >100 IC50 >100 0.7 0.8 0.5 0.4 0.3 0.2 0.1 0.0-0.2 ...........1. .-20 50 100 '150 Dose %Dose-Response Plot 45 40 35 830 i 25~20 iv 15 10 5.. ... .... .... .... .... ..1 -tail, 0.05 level of significance 0 .A 0 C 19-0 -6age 48 of 110 Pita 101_0L50812det~Ieslx Erntrd by. J. Snmer Rteviawod by. TVA / Sequoyah Nuclear Plant, intake Non-treated May 08-15, 2012 o C)EnWomuou~d I~ ftS'9 oml, Mlc.Statistical Analyses Cerlodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/812012 Test ID: CdFRCR Sample ID: TVAISQN 101 -Intake End Date: 5/15/2012 Lab ID: ETS-Envir, Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Cenodaphnia dubia Comments: Conc-% 1 2 3 4 5 6 7 U 9 10 Control-1 33.000 32.000 30.000 31.000 36.000 30.000 33.000 34.000 31.000 29.000 Control-2 30.000 33.000 33.000 30.000 31.000 29.000 32.000 31.000 31.000 26.000 10.8 33.000 33.000 30.000 33.000 31.000 32.000 28.000 35.000 35.000 32.000 21.6 36.000 32.000 36.000 33.000 31.000 32.000 35.000 32.000 35.000 33.000 43.2 35.000 31.000 33.000 39.000 33.000 30.000 35.000 37.000 33.000 33.000 86.4 34.000 36,000 32.000 35.000 37.000 35.000 34.000 34.000 33.000 35.000 100 37.000 36.000 35.000 35.000 37.000 38.000 33.000 36.000 35.000 36.000 Intake 33.000 35.000 29.000 32.000 31.000 36.000 32.000 31.000 33.000 31.000 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-1 31.900 1.0425 31.900 29.000 36.000 6.683 10 Control-2 30.600 1.0000 30.600 26.000 33.000 6.750 10 10.8 32.200 1.0523 32.200 28.000 35.000 6.677 10 21.6 33.500 1.0948 33.500 31.000 36.000 5.495 10 43.2 33.900 1.1078 33.900 30.000 39.000 7.921 10 86.4 34.500 1.1275 34.500 32.000 37.000 4.156 10 100 35.800 1.1699 35.800 33.000 38.000 3.906 10 Intake 32.300 1.0556 32.300 29.000 36.000 6.370 10 -1.844 1.734 1.599 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97072 0.868 -0.3101 0.44033 F-Test indicates equal variances (p = 0.99) 1.00787 6.54109 The control means are not significantly different (p = 0.18) 1.38493 2.10092 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.59873 0.05225 14.45 4.25 0.08172 1, 18 Treatments vs Control-2 Dose-Response Plot 45 40 35, 3025 l20 10, 5:........--....... 1-tail, 0.05 level of sIgnificance U CQ CR C4 V 0 It W I I*Page 49 of 110 File: sqnl01.050812dataxihx Entered by: J. Sumrer Reviewed by: _ I I 3T* TS Page 6 of 7 I I I I Species: Cerodpfhnia dubia Client: TVA / Seauoyah Nuclear Plant. Outfall 101 DaWly Chemistrv: Date: 0S1I- L.I Day I Concentration I Parameter a Conductivity (urnhos/cm) CONTROL"- .... " I.Alkalinity (me CaCOV/L)IF" Hardness (mg C&COVUL Temperature (C)i I 10.8% Conductivity (inhosteCm) BOY Temperature (*C)PH (S.U.)21.6% Conductivity 2~43.2% Conductivity

Temperature

(-C) ..PH (SM.-) t t-86.4% Conductivity Temperature I°) "S. 0 PH (s.u.) , DO (mgiL) y-.t Conductivity (jpihos/cni) 100% Alkalinity (mCaCO 3/L)Hardness (mng CaCO3/L)TR chlorine (mg/L) (. uo Temperature (C)Conductivity OIrnhostem) 0 100% Alkalinity Intake (mg Cac"/L)Hardness Tmg Cahlo /L)TR chlorine (mg(L) 461_________Temperature (T) Z5.0 WWIy L ..-SPge 50 of 110 Initial SOP AT 1I -Exhibit ATI 1.2, revision 06-01-11 FW" I FF,44ý. T: 5.Page 7 of 7 Species: Cerlodgwknia diubia Client: TVA / Seq ah Nucler lapjOuitfaI101 Date: a-08 --I Day!a!-3 I 4 5 A.. h .6 I.k Analyst I Concen- Parameter tration pH (S.U.)DO (me/IA S Conductivity Comhos/cm) CONTROL Alkalinity (me CaCOIL)-o------l ardness (ma CaCO 3/L.)Temperature (TC)-U ~OH (S.U.)(OIL v4 A 'Ilk A 0 -11) qAT la.0 20B IA-ek VA 13 0L I qJA2- '-+A3 10 w4-0k is.1 DO (m./L)10.8%Conductivity (amhos/cm) Temperature MC)pH (S.U.)DO (mP/L)21.6%Conductivity (wmhos/cm) Temperature (MC)OR (S.U.)':ý-Ia DO (mlL)43.2%Conductivity (umhos/cm) Temperature ('C)pH (S.U.)DO (rag/L)86.4%Conductivity (lahos/cm)_ Tem nerature (MC)1 .G 0 -1 A 1 4o b-04 I q.1110o i ~If 1~~I 1A.a M e -I aH (s.U.)DO (mg,(L)Conductivity 100%Alkalinnity Hardness'I __ .1 r Sx,0 DH (S.U.)r DO (mZIL)100%Intake Conductivity Alkalinity Hardness TR chlorine (mgfL)Temperature M')E It 1 1. a -14 , C II 1S.] -o. II I Ao -..I00 S, I I g.: 8.0 Fnl Initial Final Ital Final Inita Final-.. .... .. ...i e 51 of 110 SOP ATI 1 -Exhibit ATI 1.2, revision 06-01-11 TVA / Sequoyah Nuclear Plant, Outfall 101 -Non-treated May 08-15, 2012 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 T Daily Chemical Analyses Project number: 7875 Concentration IParameter Initial Final.Jt1Q7 I '70A Control DO (n/L)-I 7.6 8.0 Conductivity T27 Alkalinity (mg/L CaCOs) 63 Hardness (mg/L CaCo) 88M Da~~jy 3 Iiil I Final 1 7.98 8.02 294 87.9 24.8 24.9 , Da y4 Day 5 1 Day6 Initial Final Initial Final Initial Final 8.02 7.92 7.97 7.911 .06 7.871 Temperature (*C)24.91 25.21 25.01 25.1'pH (7.94 796 797 790 801 807 803 7.95 7.91 7.94 793 810 786__.8%_no_1 7.9 8.0 7.8 76 78 78 77 7.9 .0 7.8 8.0 76 76 80 108% Conductivity (pinhos/cm) ._ 297 292294 283 ROOM 291 291 Temperature (C) 250 25.2 25.2 24 250 249 24 25.3 24.9 25.1 24.7 248 248 251 pH789 7.95 7.98 797 792 01 10 8.02 7.92 7.92 7.93 7.93 10 786 9 DO.0 7.8 76 78 78 77 7.9 _ 8.0 7.7 8.0 76 76 80 21.6% Conductivity Oiunhos'cm) 280 280 276 M 286 281 Temperature(T) 250 252 252 251 250 249 248 25.2 24.9 25.1 24.7 24.8 248 249 PHISM 7.89 795 799 797 791 802 809 03 7.86 7.92 7.87 794 8.11 7.85 43.2% DO (mgL) 7.9 81 78 76 78 78 77 79 8.0 7.7 8.0 7.6 8.0 Conductivity lpmhos/cm) 265 '29 257 258 4253 MON 263 263 ITemperature(Q 25.0 252-252 252 250 250 248 250 249 25.1 24.8 247 24. 25.0 OH (SU)7.911 7.991 8.011 8.011 7.881 8.03 8.101 8.071 7.811 7941 7~3I 7991 R121 '7Ri~DOJ(WJL i 7.9 8.01 7.91 7.61 7.91 7.81 7 .7 7. 8.0 7._ 1 8.0 7.61 7.61 8.0 86.4%LUEIIUCUVILY _um" cu* I -24A 25£ ,i., 8.( 8.C25.1 24.[ 24.[8.( 8.1i 19(7, tomb 24.1 25.(rempemature I 9-oH fSUI DO (men/L)Conductivity (pimhoslem) 100%Alkalnilty (mn/L CaCOA)Hiardness (ung/L CaCO 3)roa Rsdual Moarie(pc I -Te-nerat..re 1*25.2 25.1 25.2 25.0 25.0 2. 25.0 2 509 25.1 24.8 24.8 7.92 8.01 8.4 .02 8.04 8.9 .06 8.12 8.02 8.06 7.95 8.02 7.9 8.1 8.1 7.9 8.1 7.9 7.7 7.8 8.0 7.9 8.01 195 193 192 194 188w 190%79 .-~-. --81 -7 2525.2 .25.2 25.0 25.0 24.8' 24.9 2490 24.8 2.9 25.0 1'---_r:;;__ pH (SU)DO (me/fl Conductivity (ambos/em)

I 100% Intake Alkalinity (nwJL CaCO1 Hardness (mgtL CaCO6)Total Residual Chlorine (m%/Li I Temperature (oT File: 25.11 24.91 25.21 sqn101_050812chem.xls Entered by'. C. Johnson Reviewed by-.

'En T IIIfQSculn.I. Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)Species: Pimephales proimelas Client: Tennessee Valley Authority Facility: Seauovah Nuclear Plant NPDES #: TNO020168 Project #: ¶,Kn5 County: Rhea Outfall: 101 I I Dilution prep araton information: Comments: Dilution prep 0/6) 10.8 121.6 43.2 86.4 100 Each concentration was U-'ae Effluent volume (mL) 270 540 1080 2160 2500 for 2 minutes to remove pathogenic Diluent volume (mL) :2230 1960 1420 340 0 Itreecs Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test informati.on: Organism age: L,2. .t, Randomizing template: __.__-Date and times organisms OS. a,- rit. Incubator number and were born between: shelf location: Artemia CHM number

Drying information for weight determination:.

Transfer bowl information: pH = S.U. Date/ Time in oven: _0 S_ i .._ Temperature = VA" , .C Initial oven temperature: 6o 'L Average transfer volume: 0 all Date / Time out of oven: ot,. I 0. Final oven temperature: Total drying time: ___-__I I INDO 140 Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake-_0 6S.MftS- ,_ A I% IIl.L- ,L -i"gS~-1.f" oAl V,11.5 I o r.I- M 0 040 0 At .M V1.01 -e L Q. rl.f 2 S1.2 61__ 1 Lit I100q.11 ___3 Ac. I I!- 1,__-. 14 tL400 WJi --t ,_ I -I 4 Lim. I. Iqws I Aft 11 a,"s~tl 71 1 6 rAý. t ,Mt-A 5 '17...a'-lc~7 Control information: Acceptantce criteria Summary of test endpoints: % Mortality:

07. < 20o 7-day LC , I 7.Average weipt per initial larvae: _ '().S& NOEC Z , Average weight per surviving larvae: ." 2t 0.25mg/ 'vae LOEC 1, I0C'1-ChV> 00T.*age 53 of 110 IC25.SOP AT20 -Exhibit AT20.3, revision 06 Il 0ET Page 2 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Ontfall 101, UV-treated Date: ",- 17-.t Survival and Growth Data I Day CONTROL 10.8% 21.6%AB -E 'F G. H I J K L/D /j 10 /0 .10 10 /C 10 /0 /6 to 10I 10 10 10 10 I0 I0 10 10 10 / 0 10 /. I0 10.10 ) 1b /0 10 10 /0 /0 /)' ,o ,o l 1o (' , oQ ,o ,0oo,oo ,O 4 1 o 6 oto i0 lo i0 o t. O ' o o to 1 tO)10 10 10 /0 JO /10 1 0 / 0 10_________I 1Qb 16 1Id 10 16 0 10 101 /0 to to A Pan weight (mg)Tray color code:: M~o-A Date: D.OL.ILI Analyst.ite 20 1,4-0 1q.l- 20.M 20.16 Q zojqzJ6 Iq., 1 ¶.Z,9 r*1 Date: 141 C -Larvae weight (Dig) = B -A Handcalculated.

t s.I's &On 16.Al to., %P.74 t,.\6 6 .sS i.' T Analyst- A + I .I.J Weight per initial number of larvae (mg)C / Initial number of larvae Hand calculated. Analyst: e~0*9%ýor 1'e'I'V 01 Average weight per Percent reduction initial number of from control (%) I C .-.a p -m I a a a p a a a 0.64S 0. 6%-i6. 7.Cmment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r decanted and returned, w -wounded.Comments: ge 54 of 110 SOP AT20 -Exhibit AT20.3, revision 06-0 1-l1 Page 3 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 1011 UW-treated Date: 0S-O"11.-I" I SSurvival and Growth Data Day 43.2% 86.4% 100%_M N 0 P Q R S T U V W X 0 1/0 to tb / o /01( /0 o tol Io C) o /0 10 0o 1C t / to to I0 /0110 / /o0 /0 0 /0 2 ~ / (0 i 16 t( /6 /11 to /o) Io /o) Io., 1 3 20 /0 t /01 co to /oo to 'o tO to to=,_1__ _ to to Io 1-o t to ) I'o ,o /0 to t0 5 I0 10 10 10 1 j o 't) to0 10 10 10 to 6 ~/0. n0 I0 I0 (0 iO (0,0 /0 I0010c /C/ '0 (a to ,0 /0 10 /0 10 'C) /0 l A = Pan weight (mg)Tray color code::I 4m 4 4 1 Analyst-. rst 14-4 ¢q 4.41 "J 4..A V&.5 1.4-42 14.A ISz, Date: __ __o___IIII B = Pan + Larvae g&I (mg)Analyst: ~~a 1, qs lZ q*Date: t 7" jis m-41 M24 1q.37q 14I 01.51 I.Z 20.03 A-0.C = Larvae weight (mg) = B -A Hand calculated. 5i .A (..0-A 6.31 10f ( 0. .1sO 06.fl ý..1(p ,A6&Analyst: Weight per Initial number of larvae (mag)-C/Initial number of larvae 'Hand calculated. A ___ AND'Q'Analyst: %_ I. -., of 0- v 10- o 'Average weight per Percent reduction 1-0I initial number of from control (%) 0"..). 0.510 larvae (nal) ._ 40_ .Slit)_ 217 Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, 1g = unusually large, d&r = decanted and returned, w = wounded.I II i Comments: by ge 55 of 110 SOP AT20 -Exhibit AT20.3, revision 06-01-11 , Environm tmml~TetftScIWgons, Inc.Species: Pimeohales mromelas Client: TVA / Seauovah Nuclear Plant. Outfall 101, UV-treated Page 4 of 6 Date: ,%.0%.IULM I I I I I I 1 I Day 100% Intake_ __ Y Z AA B 0 00 101 1 o00 to 0 2 /0'3 / q 101 4 (C 1(: 6 it 7 s-A -Pan weight (mg)Tray color code:: M Analyst: MW3 1S.'j.Date: -_-__ _ __B=Pan+La wJght (mg)Analyst: w AA" , ý4 Date: MM U C = Larvae weight (mng) -B -A Hand calculated. ,1 S,16 Analyst: _ A Weight per Initial number of larvae (mg)=C/,Initial number of larvae Hand calculat~ed. C Anayst .......Average weight per Percent reduction initial number of from control (%) O. to r..4 -q0.% 7o larvae (m) I.Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.ge56of11 SOP AT2O -Exhibit AT20.3, revision 06-01-11 Environm al Tesin Solutions, Inc.TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 08-15,2012 Pimephalespromdas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project numben 7875__ Not for Co im e nte.ral LaboratoryQC Ir,-. dabloogz -A-I1 a.krerkaf Swvldng.mbý.raf ,O ,. dlave(m) (%a auah'r. h mt -bum .ite g. eOW (U1 A 10 t0 13.77 19.68 5.91 0.59] 0.591 Contro 0 10 1356 20.03 6.47 0.647 0596 9.9 0.647 100.0 M6 90 Netapplie C 10 10 14.13 19.20 5.07 0.507 0.507 D 10 10 13.25 19.24 5.99 0.599 0.599 E 10 10 14.39 20.85 6.46 0.646 0.646 F 10 10 13.94 20.15 6.21 0.621 0.645 5,s 0.621 j00.0 U.645 5.1 -10.1 G 10 10 13.89 20.05 6.16 0.616 0.616_ _ 10 10 13.94 20.92 6.98 0.698 0.698 I 10 10 14.12 1965 5.53 0.553 0.553 21.6% 1o 10 13.54 19.76 6.22 0.622 0.622 K 10 10 12.70 19.29 6.59 0.659 0.624 8.2 0659 160.0 0.624 8.2 -6.S L 10 10 14.06 20.69 6.63 0.663 0.663 M 10 10 13.93 1M.58 5.65 0.565 0.565 43.2% N 10 10 13.52 19.61 6.09 0.609 0.604 4.7 0.609 340.0 0.604 4.7 43.0 0 10 10 14.95 21.29 6.34 0.634 0.634 IP 10 t0 13.26 19.32 6.06 0606 0.606 Q 10 10 14.40 19.77 5.37 0.537 0.537 86.4% R 10 10 14.46 20.19 5.73 0.573 0.570 5A 0.573 1004 &570 5.4 27 S 10 10 13.36 19.46 6.10 0.610 0.610 T 10 10 14.21 19.81 5.60 0.560 o0.560 11 t0 10 13.58 20.31 6.73 0.673 0.673 100% V 10 10 14.42 21.22 6.80 0.680 0.67t 1.8 0.680 100.0 0.671 1.8 -14.4 W 10 10 1407 20.83 6.76 0.676 0.676 X 10 10 13.12 19.65 6.53 0.653 0.653 Y 10 10 13.71 19.68 5.97 0.597 0.597 Z 10 9 13.83 19.75 5.92 0.658 0.630 10. 0.592 0.614 1.2 -4.7 AA 10 10 15.29 20.90 5.61 0.561 0.561 BB 1 10 10 13.20 20.24 7.04 0.704 0.704 Dudal 1f:Zf Dearnetts IWSD value: PMSD: intake: Dunnetets AM45 value: PMSI¶4 0,0670 11.4 IL0829 14.1 MSD -Minimum Significant Dferemce PMaD -Perent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percemt dffenunce between te control and treatment that can be declared statitically signicant in a whole effluent toxiity test.Lower PMSD bound determined by USEPA ( 0th percentile) = 12!i Upper PMSD bound determined by USEPA (90&h percentile) -30%Lower and upper PMSD boumds were deterained from the I01th and 90th percentile, respectively, of PMSD daa from EPA's WET Interlaboratory Variabil ity Study (USEPA, 2001 a; USEPA, 200 I b).USLIA. 2001a, 2001b. Final Report: Ifterlaboratomy Variability Study of EPA Short-term Chronic and Acute Whole Efilunt Toxicity Teat Methods, Volumes I and 2-Appendi.¢ EPA-821-B-01-004 end EPA-821 -B-01-005. US Environmental Protection Agneny, Cincinnati, OH.File: dx Entered by: J. srner ReVvewed by: TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 08-15,2012 0*T Statistical Analyses Larval FishGrowth and Survival Test-7 Day Growth Start Date: 5/8=2012 Test ID: PpFRCR Sample ID: TVAI SON, Outfall 101 End Date: 5115/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UIV-treated Conc-% 1 2 3 4 D-Control 0.5910 0.6470 0.5070 0.5990 10.8 0.6460 0.6210 0.6160 0.6980 21.6 0,5530 0.6220 0.6590 0.6630 43.2 0.5650 0.6090 0.6340 0.6060 86.4 0.5370 0.5730 0.6100 0.5600 100 0.6730 0.6800 0.6760 0.6530 Intake 0.6970 0.5920 0.5610 0.7040 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5860 1.0000 0.5880 0.5070 0.6470 9.929 4 0.6185 1.0000 10.8 0.6453 1.1011 0.6453 0.6160 0.6980 5.817 4 -2.130 2.410 0.0670 0.6185 1.0000 21.6 0.6243 1.0853 0.6243 0,5530 0.6630 6.163 4 -1.375 2.410 0.0670 0.6185 1.0000 43.2 0.6035 1.0299 0.6035 0.5650 0.6340 4.734 4 -0.629 .2.410 0.0670 0.6147 0.9938 86.4 0.5700 0.9727 0.5700 0.5370 0.6100 5.358 4 0.575 2.410 0.0670 0.6147 0.9938 100 0.6705 1.1442 0.6705 0.6530 0.6800 1.792 4 -3.038 2.410 0.0670 0.6147 0.9938 Intake -0.6135 1.0469 0.6135 0.5610 0.7040 10.171 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.9571 0.884 -0.4767 0.34009 Bartliett's Test indicates equal variances (p = 0.29) 6.13792 16.0863 Hypothesis Test (1-tall, 0.08) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetts Test 100 >100 1 0.06704 0.1144 0.00565 0.00155 0.01863 5. 18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 96% CL(Exp) Skew 1005 >100 IC10 >100 IC15 >100 1.0 IC20 >100 0.9 IC25 >100 0.8 IC40 >100 0.7 I050 >100 0.6 0. 8 0.4 0.3 0.2 0.1 0.0 ...- .-0.1 --0.2-0.3 0 50 100 150 Dose %Dose-Response Plot 0.7 0.8 0.4, 03, 0.1......................................... 1-tali, 0.05 level of significance

Page 58 of 110 i C. -q C4 lFile: sqniloS050l2data-uvlsix Entered by: J. Suiner Reviewed br. 1 TVA / Sequoyah Nuclear Plant, Intake UV-treated May 08-15, 2012 0 0:T 0 T ArW©r Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 518/2012 Test ID: PpFRCR Sample ID: TVA/ SQN, Intake End Date: 5115/2012 Lab ID: ETS-Envir.

Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Data: May 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: -UV-treated Conc-% 1 2 3 4 D-Control 0.5910 0.6470 0.5070 0.5990 10.8 0.6460 0.6210 0.6160 0.6980 21.6 0.5530 0.6220 0.6590 0.6630 43.2 0.5650 0.6090 0.6340 0.6060 86.4 0.5370 0.5730 0.6100 0.5600 100 0.6730 0.6800 0.6760 0.6530 Intake 0.5970 0.5920 0.5610 0.7040 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.5860 1.0000 0.5860 0.5070 0.6470 9.929 4 10.8 0.6453 1.1011 0.6453 '0.6160 0.6980 5.817 4 21.6 0.6243 1.0653 0.6243 0.5530 0.6630 8.163 4 43.2 0.6035 1.0299 0.6035 0.5650 0.6340 4.734 4 86.4 0.5700 0.9727 0.5700 0.5370 0.6100 5.358 4 100 0.6705 1.1442 0.6705 0.6530 0.6800 1.792 4 Intake 0.6135 1.0469 0.6135 0.5610 0.7040. 10.171 4 -0.645 1.943 0.0829 Auxiliary Tests Statistic Critical Skew Kurt Shaplro-Wilk's Test indicates normal distribution (p > 0.01) 0.9723 0.749 0.34802 -0.3978 F-Test indicates equal variances (p = 0.91) 1.15016 47.4683 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE' F-Prob df Homoscedastic t Test indicates no significant differences 0.08289 0.14146 0.00151 0.00364 0.543 1,8 Treatments vs D-Control Dose-Response Plot 0.6 0.7: O.5 0.5 0.4 0.2: 0.1 1-tail, 0.05 level of significance U Cn 0I age 59 of 110 File: sqnlO1_050812data-uvodsx Entered by:. J. Sumner Reviewed by:

, nmaTSinll~i.Ic Page 5 of 6 Species: VMe~hales promelqs Client: TVA / Sequoyah Nuclear Plant, Outfull 101, UV-treated Daily Chemislr:.

Date: pe6- ..I Day I U Analyst h 1 ft R~ ~ 1 2*1 I p 1 I I I I)I I Concentration 1Praer... M. =aaee Conductivity (tunrhos/cm) CONTROL UV-treated Alkalinity (ma CaCO 3 IL)Hardness (mal CaCO 3/L)Temperature (0 C)21 p(_s.U.) _.10.8% ConductivityTemperature (MC) LS.pH (S.U.) I, 21.6% Conductivity (...mhos/cm) Temperature (CC) 1.]p-H (S.,U.) -%V#Conductivity (A+/-mhos/cm) 10% Alkalinity .]________Temperature (0 C)IS-1ntPH (m CaCO8L)Hardness.(mgcaCO-L)_ TR chlorine (malL) C D C 86.4% Conductivity Tempeatur (C)TR~~D chlorne(g/L) , an, .1 a ge 60 of 110 Initial AI IOInitial Final I nitial Final SOP AT'2O -Exhibit AT'2O.3, revision 06-01 -11 9Envo~mjTetgSouknb Pa&e6 of 6 Species: Pinewhales promelas Client: TVA / Senuonvah Nuelear Plant. Outfiall 101. UV-treated Date: b4.tII.1L.. Client: TVA / Seauovah Nuclear Plant. Outfall 101. UV-treated Date: DA-0t. I~1 I Dav Analyst 3 I 4.-.4-5 I 6 Concen- Parameter tration I PH L.U.DO (mizL)Conductivity (uxmhos/cm) g 1 CONTROL UV-treated (AikaCinhty (mg CaCQIL)Hardness (Mg CatQ3jL)510 Le. 40 283 M9 Temierature (*C)pH (S.U.)DO (mg/L)10.8%Conductivity Temnerature (0C).pH (S.U.)"As 11e 1 /48 11 DO (Mg/L)21.6%Conductivity (=mhos/cm) Ten..... ur ..... "('C "-4--------------. I)OH (S.U.)*

t" DO (mg/L)43.2%Conductivity (iimhoslcm)

Temperature (00)8.0 C1.8- I-Lq.l- -af M 4.&P .Wo*1. o I AO I_ Xq 115S.0$-a %10 11 %IA 1 4-24W 19-1 0.0 1410 1 I ecl DR (S.U.)DO (mu/L)86.4%Conductivity I 0.s 6 230 Temperature (C)-OH (S.U.)I.DO (ma/L)I-100%Conductivity (sunhos/cm) Alkalinity (Ing CaC03/L)Hardness (M9 SC0C3/L)j TR Chlorine (mg/L)Temperature (0C)II3 I0-i pH (S.U.)I149L- M I q,94 L'K-k 1 IS.I-j 24-3 1 qO I G-1W 114,62- 1 7 DO (mu/UL 100%Intake Conductivity (Mmhos/em) Alkalinity (ing CaCO3/L)Hardness (M& CaCO3IL)TR chlorine (mg/L)Temperature CC)md.p.ni.~t I..y ge 61 of 110 j I U -~ L 5 iInitial i Finl 1 Inta t Final IVdyS 11 14.(.At I -LI i Initial Final Initial Final SOP AT2O -Exhibit AT'20.3, revision 06-0 1-11 TVA / Sequoyah Nuclear Plant, Outfall 101 -UV-treated May 08-15, 2012 Pbnephafespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number. 7875 Concentration Parameter _ __ _ Day I___Day_2_Day 3 Day 4 D5 Day6 Initial Fial Inia al Initial F l Initial Final Initial Final Initial inal Initial Final PH(SU) 788 7.98 7.98 7.97 7.69 7.97 7.62 7.92 7.69 8.07 7.51 DO (iMRL) 79 8.0 7.8 8.0 7. 1 8.0 69 7.6 6.5 7.8 6.8 Conductivity (izmhos/cm) 31633 300 304 f*-' 306 3 309 304 Control Aarmity (mg/L CaC.() 623 64 64 : 63 62 Hardness (nig/L C&CO 3) __ 7_ __ 7m_ 89 M 89+_ i 89 -O 89.Temperature (:C) 249 24.8 24.9 24.8 24.8 24.5 24.8 24.6 24.9 24.8 24.7 24.7 24.9 24.7 PH (SU) 7.88 7,84 7.98 7.82 7.98 7.85 7.98 7.67 7.94 7.60 7.91 7.63 8.09 7.50 10.% DO (mra/L) 7.9 7.9 8.0 7.7 7.9 7.8 8.0 6.9 8.0 69 7.6 66 7.8 6.Conductivity (iunbos/em) 305 301 295 3 283 1 299 298 Temperature (!Q 25.0 24.8 25.0 24.9 24.8 24.5 24.8 24.4 25.0 245 24.8 246 25.0 24.5 pH(M 7.89 7.90 7.99 7.82 798 7.85 7.98 7.68 7.93 7611 79 761 .10 7.50 DO (mgL) 80 8.0 8.0 7.6 7.9 T8 8.0 6.9 80 68 76 65 7.8 6.8 2 1.6 % C o n d u ctiv ity ( tim b o s e m ) 2_1 l289 2 90 2 8 3 W 1 j. M 2 6 9 2 M 2"7 2 484 Temperature CC) 250 24.8 25.0 24.9 24.8 24.5 24.8 24.7 25. 247 24.8 246 25.0 pH 789 789 799 7.83 7.97 7.84 7.99 7.68 7.88 763 786 760 8.11 7.50 DO (M/L) 80 79 80 7.6 8.0 7.8 8.0 6.9 7.9 6.8 7.6 65 7 6.8 43.2% Conductivity (tunhos/em) 266 263 M 259 2 .60 248 259 266 Temperature ('C) 250 245 25.1 24.6 24.2 24.6 24.8 24.8 25.0 247 248 246 25.0 24.5 1H (SU) 790 790 800 7.82 7.94 7.88 8.00 7.68 7.81 773 7.79 765 8.13 7.50 DO (m2/L) 79 79 80 7.6 8.0 7.8 8.0 6.9 8.0 68 7.5 65 7.8 6.7 86.4% Conductivity (pmhos/em) 221) 215 213 215 WT.S A 207 221 220 Temperature CC) 25.0 24.7 25.1 24.6 24.8 24.6 24.9 24.6 25.0 24. 24.8 24.9 25.0 24,6 PH (SU) 792 791 802 7.84 7.94 7.89 8.02 7.69 7.78 7.69 7.76 7.691 .15 7.50 DO79 9 80 7.7 8.0 7.8 8.1 6.8 8.0 7.0 7.6 6.5 7.9 6.7 Conductivi ( hos/m) 199 198 197 201 --m 189 199 200 100% Alkalinity (mg/L CaCO3) 79 79- 79 *,A Hardness (Qg/1L CaCO3) 81 81 ..81 Total Residual Chlorine (maL) <0, 10 0.1j <0.I0 _Temperature (LC) 250 246 25.1024.5 25.0 24.7 25.0 24.6 25.1 24.8 24.8 24.8 25.2 pH (SU) 794 795 805 7.85 8.01 7.91 8.04 7.76 7.99 7.71 7.94 8.16 DO (m./L) 8.0. 7.9 8.0 7.6 8.2 8.0 8.2 6.8 8.2 7.6 7.91 6.6 onductivity (pjmbos/cm) 194 195 189 19618, 191 100%AIntake Alkalianty(WgILCaCO

3) 79 77 -7;-. 79 Hardness (mgf CaCOs) 81 -79 .. .1 S Total Residual Chlorine (me/L) .<0.10 -, <0.0I_ _ Temperature (C) 25.11 246 25.2 24.51 25.1 2.7 24. 4. 25. 24.8 24.File: sqnlOl 050812chen-UV.xIs Entered by. C. Johpson Reviewed by-.

I I!!i!Page IR Page t of.J_0 B SSETS~ EnvIsrn~mntutThsUng Total Residual Chlorine (Orion Electrode MethOd, Orion 97-70)Matrix: Water, RL = 0.10 mg/L Meter: Accumnet Model AR25 pH/Ion Meter Analyst I I Date analyzed Calibration: I I, Acid reagent: L Tk. II.IIII]Nw a r0.10 mg=L TOr msL sloe Note: For samnples with a residual chlorine of > 1.0 m/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.Laboratory control standard: Reference standard True value (TV) Measured value (MV) %RS--MV/TVx 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%),,)¶ .Duplicate sampl recislon: Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S -D) /[(S+D)/2j) x 100 number I (MO.' (acepuffi' ".1%)SI DuplicateD Sample measurements: ,, Sample Sample ID Sample characteristics Residual chlorine number (m9rL)Reagent Blank A .<mg~sng- 0 fw"Q~t LiuA- &arWkIi. VA. 4 _ _1_9 ipi V265 ~2 Ot- &WA PA&CIr wk 61 4031005 Note: All samples were analy{el in excess of EPA recommended holding time (1'5 minutes) unLess otherwise noted.Laboratorv control standard: ,, Reference staadard True value (TIV) jMeasured value (MV) % RS-=MV /TV x 00 j number I(rag/I) j(nag/L) (acceptable ra~nge = 90 to 110%)*2 t 0.50 o. -'0 oQ',*/f P, AAap I I I Reviewed by i IZ " Date reviewed 1 08- I-_SOP CS -Exhibit C8.1, revision 06-01-11 Page 63 of 110 Page 1,0 Page 'L of 7, intnEnTaI~dSeuo&Ic Total Residual Chlorine (Orion Electrode Meth6d, Orion 97-70)Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent: [Acid reagent:)Analyst I Date analyzed [ ]Calibration: Iodide reagent:II Acid reagent: [II Z ]F sam.les wth a resdual ch_-- e of> .0 tecalibranrne muSlope-Reference standard number Ems 4, Mote: For samples with a resida chlorine of > 1.0 rag/L, the calibration range must be adjusted to bracket the chlorine levels of the samnples.D 4 0-oov I o~R I Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number, Je, Blank .....iZOS.6¢0l. "_'_h ________I1 dae.-, .t, 1u7-DS 0 -Orkd6,, 0.:044d, w& 6 ,,0 1%NotAsps wr nm m ulth Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.Reviewed by I-e#Date reviewed I _*_"1L.SOP C8 -Exhibit C8.1, revision 06-01-11 Page 64 of 110 I?Page Page I of isa EnlnmnalaThgSaur Total Residual Chlorine (Orion Electrode:Method, Orion 97-70)Matrix: Water, RL'= 0.10 mg/L Meter: Accumet Model AR25 pHfIon Meter Analyst [ II, Date analyzed LoI1Il-, Calibration: odide reagent: I N Acid reagent: Lim I WM N ofe r e r s a n ta r e iu a hr 0 .1 0 m g I L t 1 .0 0 m u t b d S lo p e l IZI ,art l 1X to" I -4S.'r1, i Note: For samples with a residual chlorine of > 1.0 mgiL, the calibration range must be adjusted to bracke the chlorine Iees of the samples.Laboratory ijonfrnl .alandard: I Reference standard True value (TV) Measured value (MV) %RS=MV/TVx 100 number (rag/L) (mg/L) (acceptable range = 90 to 110%)L 0.50 o.sto o Duplicate sample precision: Sample Sample ED Sample characteristics Residual chlorine [%RPD = ((S -D) /[(S+D)/2J) x 100 nube (mg/ (acceptable range -:h 10%)I______ Duplicate D co.eoso, Sample measurements: ..... ..Sample Sample ID Sample characteristics Residual chlorine number ,, mg/L)Reagent Blank Co.o o 04'______ ___________no .ItjS Co a. 412 emal~.~ eAS ym% no~ C~~olor %Zt 4 L4.r n flO 4 C. 0. 0" C97/ i t) no cointe , pu -,... c."ooqt+ ow d ..ux;, 0 4 f. 0.a6.0 ,61'8 3 I.OG-"0. 06 f\- LOi'mLAIU 4 ,.(6 e lav, o. (0.Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.Laboratory control standard:. t True value (TV) Measured value (MV) % RS =MV TV x 100 number (Ing/L) (acceptable range -90 to 110%)0.50 ad 6S& 't.Revewe byII izz Date reviewed 105Ii....SOP C8 -Exhibit C8.1, revision 06-01-11 Page 65 of 110 I Page ZX Page-of IL 0FT Total Residual Chlorine (Orion Electrode Method, Orion 97-70).Matrix: Water, RLf=f0.10 rng/L Meter Accumet Model AR25 pH/Ion Meter Analyst [I a Date analyzed LM610 .l, M II odide reagent: , Acid reagent: I mo m. mj Calibration: S0.1m 1.00mg I Sop e I Reference standard number I *AAd 5i3aftI I Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.Laboratory control standard:. Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (rag/L) (mg/L) (acceptable range = 90 to 110%)I 1066 4 0.50 6.LtCO qz.on Duplicate sample precision: Sample Sample D1) Sample characteristics Residual chlorine %RPD = [(S -D) /[(S+D)/I2) x 100 number (mg/L)(°I DuplicateD Sample measurements: _Sample Sample ID Sample characteristics Residual chlorine__ _ _ _ _ _ (mg/L) , Reagent Blank "W" 1e. c4r clgs 40 p t0. docrL Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.Laborato control standard.Reference standard True value (TV) Measured value (MV) % RS = MV /TV x 100 number (mg/L) (rag/L) ,, (acceptable range -90 to 110%)INM 0.50 o. 51l to 7. 1 Reviewed by I A-o'Date reviewed -SOP C8 -Exhibit C8.1, revision 06-01-11 Page 66 of 110 I Page Page _L_- of _I S 0 9 Total Residual Chlorine (Orion Electrode: Method, Orion 97-70)Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter I Analyst ft-V Date analyzed I " -17E-]Calibration: I odide reagent: I ij f I. Q' I Acid reagent: E" A".0 to + I 1.o9 mgL 7- " Slope: Reference standard number 1 /Lt caliaon rnemt be a t , I Note: For samples with a residual chlorine of > L.0 rag/L, the calibration range must be adjusted to bracket the chlorine levels of the samples, Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)I ____,____...._ s0 0.50 105-67.Duplicate sample precision: I Sample number Sample ID Sample characteristics Residual chlorine % %RPD -{(S -D) /[(S+D)/2]) x 1001 I oan""&h I (Mg/L) -, I (acceptable range =- 1o%) ...I Duplicate S 4-01-.&(, Awn.I Samale measurements: I I I I I Sample Sample ID Sample characteristics Residual chlorine number .... Reagent Blank .O.00?2f/ro o~h'i, L.Os /o.0005"0FAQ.HIJtUxe 0 ici ,J6/ -£0 .O061fi5&Nt CAK,4 fVL4 ft< c.,b/ 6O,0oo760E63LdAtJh YA_._ 1 o , -5LJ &/a 10 4O 060 L as -L o%( AeLPetn& d"N~L~. to, 800W600 7-ote1 A2 sl05 l excess If EA 'l I INOte: All samples were analyzed in excess of EPA recomended hodn ie(5mnts nesohrientd Reviewed by I .Z Date reviewed It -'L Q SOP C8 -Exhibit C8.1, revision 06-01-1 Page 67 of 110 Page Page 2--of 2 Z Total Residual Chlorine (Orion Electrode Method, Orion 97-70)Matrix: Water, RL= 0.10 nig/L Meter: Accumet Model AR25 pH/Ion Meter Analyst[ L. I Date analyzed L ii I iZ Calibration: [4 xoide reagent: F?. It, Acid reagent: I --0.10 mg/L 1.oo mg/L Sipe I Reference standard number I T w --, Note: For samples with a residual chlorine of > 1.0 mgfL, the calibration range must be adjusted to bracket the chlorine levels of the samples.Laboratory control standard: Reference standard True value (TV) .. Measured value (MV) % RS = MV / TV x 100 number .(rag/L) (rag/L) (acceptable range=ff 90 to 110%)I gcos jou q 0.50 f &Duplicate sample grecision: Sample Sample ID Sample characteristics Residual chlorine %RPD = [(S -D) /[(S+D)/2]) x 100 number I (a(a ar *G-S11 .1m V'k!h Jm 1. N s /o.0ot22.Duplicate ._ .Sample measurements:. Sample Sample ID Sample characteristics Residual chlorine number (mJ[/L)Reagent Blank ." OS/I4' 1VA .,tJ -J ,1efw , c 1, V_' .,2 #/10 1 Lr no cdiw ek ___ ___R) 4DIVI 6W ny 0,1A-ml-Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS MV /TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)17 1 o1 q 0.50 , qlq All. f Reviewed by I I Z Date reviewed SOP C8 -Exhibit C8.1, revision 06-01-11 Page 68 of 110 r7 0 0 0~D Fnvirci~meut.F1~.ttng S1oimgja~. Page 30 Page L___of )Analyst II71 Date analyzed [~ II Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH = 4.5 S.U.Time initiated [ ots Time completed [34j- S I I I I I Ii I I.11 Ii[I Li U T7-rant normality and mutiplier determination: pH of Normality Normality (N) of HS0 4 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na2CO 3 x 0.05)IE = (N x 50000)l 100 ml sample water reference standard ml ml ml -0 .25/E =Nx 500 4.5 S.U. number number (E) (acceptable range = 0.0180 -0.0220)'4.4C '4 s lol 1 6.6 1 t .0 I to C.ore0 t 16*4 Laborafory control standard: Reference standard True value Sample Alkalinity (MV) % RS-=MV /TV x 00 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range i (g CaCO.3 L) (ml) ml ml ml -m9 to 110%)=N t~wlo to 10o0o 1- t l.c I q. Cx,, too ,,.o Duplicate sa leprecision: Sample Alkalinity %RPD =Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) ((S -D) /[(S+D)/2]1 I 100 number (ml) mL. ..ml ml (acceptable range *10%).Duplicate (B) -1 .3 D 3&D Sample measurements: .Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (Ing CaCO3/L)05.o042., rn .,. too Uz.1 3j.0 C.1 10. C3 o06.O!'.. I.A k I I.*c 4 .CI C3 1 05.o..06 _ Ml.6 i. .[ 1_10 O___._,t- .j _ .1.___ d.4 &..3 4=,, Reviewed by: I L ..-Date reviewed: O{ .0) 42.Page 69 of 110 SOP C6 -Exhibit C6.1. revision 06-01-I1 I Envlr@nreg Testing Sahillons. Inc.Page 31 Page I of Analyst I .F Date analyzed 1 0a. ItI I Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaCOQL Titate.samples to pH = 4.5 S.U.Time initiated 441C, [Time completed stutt Titrant no mal and multiplier determination: pH of Normality Normality (N) of H-2S04 pH Factor or Multiplier Delonized Titrant check Begin End Total = (5 ml NazCOl 3 x 0.05)/E (N x 50000)/100 ml sample water reference standard ml ml ml = 0.25/E = , Nx 500 4.5 S.U. number number (E) (acceptable range= 0.0180 -0.0220)["It 12St .I 4 sb.! t 0.0207 ooo*Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (rag CaCO 3/L) (acceptable range (mg CaCO 3/L) (ml) ml ml ml = 90 to 110%)=N 637 100 100 tV7.ZJ .q/. 10.4. teoo,[o Duplicate sa l e precision: Sample Alkalinity' %RPD -Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) ((S -D) /[(S+D)/21] x 100 number (ml) ml ml ml + * (acceptable range = A 10%)Duplicate (B) D .L Matrix sike recove__ _Reference standard Spike value Sample Spike alkalinity (A)number (SV) volume Begin End Total Multiplier (Ing CaCO 3 IL)(mgCaCOs/L) (ml) ml ml ml ...Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (rag CaCO3/L) MV = A -B (acceptable range (rag CaCOsI,) 75 to 125%)So Sample measuremnents: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)O'5-. ? t- fl" n. 100 %.L2 -1.vI c.r 16464 06. )it 8 -___U 31. cs.s v____3 05 i...2~ ..... _o _,, 1. I us. _ _ 0.)___1 0'15 t.l O_________, _ _ _ l.. t%.C CAt C3#. om L.' ..t3__ _ _ _ _ _ __ _ _ _ Jt. o, .. _ _ _ _ _ _ _ _ _ _ _ _Reviewed by: I Date reviewed: [Ia 0Sr4. IL. -I Page 70 of 110 SOP C6 -Exhibit C6. 1. revision 06-01-11 I 0ET 40nhnws~~fpoA~%m Page )_J _Page 2 of._J I I D ýnalyst 17 : ]Date analyzed F 4-c.tit Alkalinity (SM 2320 B)Matrix: Water, RL -1.0 mg CaCO 3/L Titrate samples to pH.= 4.5 S.U.Time initiated Time completed I Titrant normality and multiplier determination: I I I I I I I I I I pH of Normality Normality (A) of H 2 So 4 pH Factor or Multiplier Titrant check Begin End Total f (5 ml Na 2 CO3 x 0.05)/E f (N x 50000)/ 100 ml sample water rneafrenee- ._drd ml ml ml -0.25/E Nx 500 4.5 S.U. number nunmt-- (E) (acceptable range = 0.0180 -0.0220)Laboraltor control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV I TV x 100 number (TV) 1volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (rag CaCO3/L) (ml) ml ml ml =90 to 110%)32u~$ 100 o 'l- 11. 4 10 l.q too Ioo Duplicate samole precision: Sample Alkalinity %RPD -Sample Sample ID volume Begin End Total MIltiplier (mg CaCO/L) {(S -D) /[(S+D)t21j x 100 number (ml) ml ml ml I , I (acceptable range f 10%)c .o'b-M oo V .1 I.1: 4.o 1...5 .T Duplicate(B) D :1.7 c., D 3 Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A)number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)(im CaCO 3/L) (ml) Ml ml ml ZW"s~ 1037 so too ,s.C ZC. o4 ,o. 104 toG Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (iag CaCO 3/L) MV -A -B (acceptable range (mg CaCO3/L) = 73 to 125%)Q 147 Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (rag CCO/)o_-_r___rV._ i UV 2 106 U. S sz. ,I ti1q 43..... 3LS 311.7 94 e._OS-t- Q. A. L4_ ___' ___~ Ca_ __bs- IV t-1. 9.l 1 ý. _.__ C3 t Sccru.t.o I _h__o3ih_ Ice PA*+M.Q0ff. t¢o qs oo ,M ýfff s s, ,.1 4.3 0" MQ4#. b"1 3.1 141A 1.1 -I I I I Reviewed by: I A Date reviewed: [Ili rf'l Page 71 of 110 SOP C6 -Exhibit C6. 1. revision 06-01-11 0ET Page IN Page ._ of S Analyst 3-Z Date analyzed dLit II Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to.pH = 4.5 S.U.Time initiated Time completed [ I]T711rant n gpaftiAand ,.guiniia, daaprni,.. 1 Normality Normality (N) of H 2 S0 4 pH Factor or Multiplier Deionized TitranT" ---_in End Total = (5 ml Na 2 CO 3 x 0.05)/E = (Nx 50000)l 100 ml sample water reference standard -ml m" m0."- Nx 500= 4.5 S.U. number number E)- (aEceptablera .220)Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (rng CaCOs/L) (acceptable range (mag CaCO 3/L) ( m) nl il ml 90 to 110%)U51,w toT1 100 100 9.4 IS.3 9,7 10.4 lot ooY Duplicate sam ple precision: Sample Alkalinity %RPD =Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL) {(S -D) /[(S+D)12]} x 100 number NO(ml) ml ml ml (acceptable range = k 10%)1,6 S -O io, I.) :S.$ to.o 10A S 16o Duplicate (B) 7 17ts 13D 6- t Matrix spike recover: "__Reference standard Spike value Sample Spike alkalinity (A)number (SV) volume Begin End Total Multiplier (mng CaCO 3 L)(mg CaCO 3 IL) (ml) ml ml ml 14SS 13"7 S6 1O :it,3 '4X.0 1q.1 lO.'4 ISO Sample alkalinity (B) Measured spike value (MV) % R =MV /,SV x 100 (mg CaCO3/L) MV=A-B (acceptable range (mg CaCO3/L) = 75 to 125%)Sample measurements:au Sample volume Begin End Total Alkalinity Sample number Sample ED (ml) ml ml ml Multiplier (ing CaCOs/L)O...0-.b SaA*.-+ 6Wa to 0.0 q t+ e too 06Lq 141 ___1.41_____ Inm .n/ O-L ,Pk IQ0 101 k __ _il bb 1,t -7q 2- 32,_ q 39.9 3 A _ lot______...',Lo. _, __7_\"L.O .l,,_ Oi"LU-- I p ,ts&ý l o.6 614.3 A-'i -' 77 V0 .1'4%3 49.11 3.Reviewed by: [II Date reviewed: I 0S 1a 3'I.Page 72 of 110 SOP C6 -Exhibit C6.1. revision 06-01-11 0:T Page .1 41 Page __ of_ I Time initiated Time completed I S.*ILDL Analyst =Date analyzed I __.__' __2Tirant normalitv and multiplier determination Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH = 4.5 S.U.of Normality Normality (N) of H 2 SO 4 pH Factor or Multiplier rant cheek Begin End Total -(5 ml Na 2 CO3 x 0.05)/E (Nx 50000)1100 ml sample water reference -ml ml ml = 0.25/E NX 500 4.5 S.U. number number (E) (acceptable tange -0.0180 -0.0220)Laboratory control standard: Rference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number j (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml 90 to 110%)1o0 3.8 g o t too Duplicate ple precision: Sample Alkalinity %RPD -Sanmple Sample ]iD volume Begin End Total M .ltiplier (mg CaCOS/L) {(S -D) /t(S+D)t21) x 100 number (m]) ml ml ml (acceptable range= .10%)Duplicate (B) 17,Z Z0.q D 77*1 i. 2.¶ .7 ___._____I I I Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO-3 L) 0% 7- __ ___?4 " 2..q .4,!"f 1. (3~ '&~ "7'% -Uj-1/AAt A. -L 37.QA. I.L. -7__ _ _ _ _ _ '46.7 fis '. $, .S' 7R i-2-moeaO. am &t, O to% 0.0o 9.1 4.1 2L s 0.._ 71 4.1 _93_ _-I Lo a qI r A w'u.)W 10 iOWt I _______ __C.___1____ Reviewed by: AII Date reviewed: I j .-j ._Page 73 of 110 SOP C6 -Exhibit C6. 1. revision 06-01-11 0:T 0ýar"rIaf tm W Page 3S Page' _ of .6V-Analyst LIII 1 Date analyzed Lli.t. ,i Alkalinity (SM 2320 B)Matrix: Water, RL -1.0 mrg CaCO 3/L Titrate samples to pH = 4.5 S.U.Time initiated i Time completed*5*W., 12.rdrant normalily and multiplier determination: Normality Normality (N) of H 2 SO 4 pH Factor or Multiplier Deionized Titýan k Begin End Total (5 ml Na 2 CO 3 x 0.05)/E = (Nx 50000)/100 ml sample water reference standard"I ml = 0.25/E .=N 500 4.5 S.U. number number Ygeptable range 0.0180 -0.0220)Laboratory control standard: Reference standard True value Sample Alkalinity (MV) %RS--MV/TVx 100 number (TV) volume Begin End Total Multiplier (Nrg CaCO 3/L) (acceptable range (ag CaCO3/L) (ml) ml ml ml I= 90 to 110%)100 100 'u.S u.o q. I Duplicate s le precision: -, Sample Alkalinity %RPD =Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) {(S -D) /[(S+D)/21} x 100 number (ml) ml ml ml _ _ (acceptable range -:h 10%)4AQ iiii S b1-uplicate (B)t3 33. D~ _.Vanenle mfur~m1~nft! Sample volume Begin End Total Alkalinity Sample numbr Sample ID (mJI) ml ml ml Multiplier (Mg CaCO-IL)vzsa VA JWW 10Wb 5D 3$64.0 LID 1 0 4.q I'1& $ l .I I 7.......%-I \.L _ ._ SL #4. 3 l I Ii I _ S.( Vi 77________- 2.4 jq I -- -r-~ 2,...!.* iL~' .6 ..__ ____ ___i .ial 4, 1o ., z .g _ _ _ _ _ __.1-Reviewed by: I A 7 Date reviewed: I t.-., iI.Page 74 of 110 SOP C6 -Exhibit C6.1. revision 06-01-11 S S 0 0~l EnvbDnmOfltiIThSfIflg S.iutio.i,. Inc Page 3 1 Page .C of L i!I I i Ii!AnalystI .[ t 7 Date analyzed [as. 12-1Z Titrant normality and multliler determination Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH = 4.5 S.U.Time initiated Time completed eto -Normality Normality (N) of H 2 S0 4 pH Factor or Multiplier D jn check Begin End Total = (5 ml Na2CO 3 x 0.05)/E = (N x 50000)1100 ml sample water reference s -ml ml ml = 0.25/E = N x 500=4.5 S.U. number number (acceptable range = 0.0180 -0.0220)Laboratory control standard: ence standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (Mg CaCO/L) (acceptable range SI (mg CaCO 3 L) (ml) ml ml ml g9 to 110%)I I I X16 Wy 100 00 I5.t 2414.7 rII.S Io.1 -0 .. .o DapUlicate sample precision: Sample -Alkalinity %RFD=Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) [(S -D) /[(S+D)/2]1 x 100 number (ml) ml ml ml _ (acceptable range =L 10%)I LEf..T, o gjm 1IL.t L lob e.'a I-& -2 164 S Duplicate (B) D, Matrix spike recoe ry_Reference standard Spikevalue Sample Spike alkalinity (A)number SV) volume Begin End Total Multiplier (mg CaCO 3/L)...(rag CaCO3/L) (m]) ml ml .ml a 1'57 SoT7 16_0_ %_,_ 1_.3 _7. 9 7S Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (mng CaCOj/L) MV = A -B (acceptable range (mg CaCO 3/L) 75 to 125%)Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ED (ml) ml ml ml Multiplier (mg CaCO3/L)7.sor. it), c" 100 ko.1 LS. 4 10- 4O.4 51 3 _._o___ s.?5. 2.0 7j Sq. 53 Cxo1.0 I I j ,. T 1 g¢,, 4.- 170,, , 3'4, S , 3 -.3 ~ ~ N f____ Se*~ILO 9CS J1 I0~24SORtoo 1q.c~ILO ¶0 4%L.4 3.4 37 31 _ _._ 4 _ S Reviewed by: Date reviewed: I Es,-t'LI Page 75 of 110 SOP C6 -Exhibit C6.1. revision 06-01-11 q 0 0 0~ mIronmODtI1Te5dn9ScJution~. Inc.Page 21?Page _ of-Time initiated t ]Time completed I Analyst I 're III Date analyzed I 6 j.3 jj-Alkalinity (SM 2320 B)Matrix: Water, RL 1.0 mg CaCO 3/L Titrate samples to pH = 4.5 S.U.Ho Normality Normality (N) of H 2 SO 4 pH Factor or Multiplier Dei t check Begin End Total = (5 ml Na 2 CO 3 x 0.05)/E (N x 50000)/100 ml sample water reference --7nad ml ml ml = 0.25/E NX 500 4.5 S.U. number number ----._ (acceptable range = 0.0180 -0.0220)Laboratory control standard:. Reference standard True value Sample Alkalinity (MV) %RS=MV/TVxOO number (TV) volume Begin End Total Multiplier (Mg CaCOfL) (acceptable range (rag CaCO3/L) (ml) ml ml ml -90 to 110%)103"7 ~ ~100 100 e ¶.* I Duplicate samn le precision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (rag CaCO 3/L) ((S -D) /[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range -k 10%)__]Duplicate (B) DIo l&9 29 " Sawmle measurements: Sample volume Begin End , Total Alkalinity Sample number Sample ID (ml) mi ml ml Multiplier (mg CaCO 3/L)J.,iSD _'_ 2 ZIA U1.4 v.i 2.7 h) %.q ii.I ,. 3 5 31's 3'L.,t :.A I 1Zo a os.ffnb n I Z 4.M 6A.. IS) '3100 ll( a 1t.0 2 4O.3 q4Cq C.: 1 _ __.00 L 2.._asa $3 .o 1.CA C.- 3t00 , I. _ _ 10. l'.I 1. 0 -'ciao.._t I .Oh J7 0 3 1%. 1 n. 3.$ __ _" 120SO.6q I Aalur , i.-s -ac Reviewed by.LZ~ZZ Date reviewed: I a. 1z I Page 76 of 110 SOP C6 -Exhibit C6.1. revision 06-01-11 SET Page 3 Analyst [I777 Date analyzedL4IiiI] Alkalinity (SM 2320 B)Matrix: Water, RL 1.0 mg CaCO 3/L Titrate samples to pH = 4.5 S.U.Page _I___.of S6 Time initiated Time completed L i I qplt4x I PH of Normality Normality (N) of H 2 S0 4 pH Factor or Multiplier Dei-5m3d-n itrant check Begin End Total (5 ml Na 2 CO 3 x 0.05)/E (Nx 50000)1100 ml sample water refer-r" ml ml ml = 0.251E --=Nx500= 4.5 S.U. number numnfr- (E) (acceptable range -0.0180 -0.0220)i --i-"-M wgS.tZ. 1 00 Laboratory control standard: Reference standard True value Sample Alkalinity(M %RS-MV/TVxI00 number (TV) volume Begin. End Total Multiplier (mug CaCO 3/L) (acceptable range_ (mg CaCO 3/L) (ml) ml ml ml -90 to 110%)XNSS 16r7 100 100 ...$ 3%.- 0.'7 1 o.4 lot 1 1.' o YO%I I I I" Ii i Sample measurements:,, -Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml Iml MIl Multiplier (mg CaCO/L)1L I OSl -61 i golyr 2 Z 6 V0 31.7- 14. ( 14.,q 7 1 t.617L04 .4tJ~ L__-3 391.2 -35 4__ 7S__ C tsk. ffn4sM v too *3 .2, 41.t TG, CZ____-----____' --s. I3.,its Reviewed by: I IAA -Date reviewed: EI 0.-Z1I~Page 77 of 110 SOP C6 -Exhibit C6. 1. revision 06-01-11 TS Page-Page. j of I Time initiated Time completed [ Total Hardness (SM 2340 C)RL = 1.0 mg CaCO 3/L Analyst Date analyzed 1is Thrant normality and multiplier determination: Titrant Normality cheek Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml mi = 0.Z/E = (N x 50000)/50 ml sample number number (E) (acceptable range = 0.0180 -0,0220) = Nx 1000 su 1490 XNGS , jt 0.0 W0,0 O.0 ..... 6o z2 6 LaboratorY control standard: ._.............. Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mag CaCOa/L) (acceptable range (ag CoCO) (m) ml ii ml ml -- 90 to 110%)XtSS %033 40 50 0.0 IZ~A Zo .0~ %4o 40.*Y Duplicate sa l ie precision: .........Sample Hardness %RPD =Sample Sample ID volume Begin End Total Multiplier (mag CaCO 3 L) 1(S -D)/[(S+D)i21) x 100 number .. (mi) ml ml ml /_505lZ ____ Io So it _ _.0 _._, _.. s 4o Duplicate (B) D '.Matrix spike recovery: ______________ Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)(mg CaCOIL) (ml) ml ml ml Xt&AS 143 4O so 14-0 14.0 4,0 Z00, Se Sample hardness (B) Measured spike value (MV) % R MV / SV x 100 (Mg CaCO3/L) MV A -B (acceptable range (rag CaCO/L) 75 to 125%)4tto o e .Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOh/L)TV-ND Blank (should be -0 mG CaCO3.L) so *A z *o 0_____,___ 0~- b"6.?A ____ ______0!C 0........ _.. __ _ D_3..._6' ,- 313 *.os.iu ftP>Flol4I4Os used, sample must be diluted. Reviewed by: i. I]Date reviewed 10!5 O-I , SOP C7 -Exhibit C7.1. revision 06-01-11 0ET Page ý17 I I Total Hardness (SM 2340 C)RL = 1.0 mg CaCOQ 3 L Page l of _.Time initiated 0LI 1 " .I Time completed i!g3 Analyst Dateanalyzed I I*Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJ/L) (acceptable range (rag CaCO,/L) (ml) ml ml ml -90 to 110%)13ss 40 50 10.1 Iz. I Lo tq.s 40 Duplicate s e recislon: Sample Hardness %RPD-Sample Sample ID volume Begin End Total Multiplier (mig CaCO 3 IL) ((S -D) /[(S+D)/21) x 100 number (ml) ml ml ml ,I. , Duplicate(B) Z .0 D ------ o , Matrix spike recovery: Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (rag CaCO3/L)(rag CaCO 3/L) (ml) ml ml ml Xtass 1*33 4-0o So A l.i 4-o It.s 7 Sample hardness (B) Measured spike value (MV) % R = MV/ SV x 100 (Ing CaC-3/L) MV = A -B (acceptable range (mg CaCO(WL) 75 to 125%).40.31 37.5%Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml MulUiplier (mg CaCOA/L)TV=ND Blank (should be = 0 mg CaCO3/L) so 66. o 4 0 00 I J_______ 4.S'.____ _ -12,71 '31.i f.S SIZT. 1,J1 q.q,80acz__,_ 46.5 4.o q~ q Q1 C-t 4 m,& .__S__.,- Uo.3 _4.5_ _ __S.MdU 11. 6 4.6 .1 q P T!9 >;gS W9 f $10 is used, sample must be diluted.Reviewed by: I iI , ]Date reviewed I { S Is SOP C7 -Exhibit C7. 1. revision 06-01-11 0ET Page 9 Page ?. of 4 Time initiated Time completed*'p rotal Hardness (SM 2340 C)RL = 1.0 mg CaCO 3/L Analyst L i Y Date analyzed oS. W MII Titrant normality and multiplier determination: [ Normality check TBegin End Total Normality (N) of EDTA pH Factor or Multiplier reference st~an-6daýt-ml ml 0.*2/E= (N x 50000)150 ml sample number number I (acceptable range = 0.0180 -0.0220) -Nx 1000 Laboratory control standard: ..Reference standard True value 'Sample Hardness (MV) % RS =MV/TV x 100 number' (TV) volume Begin End Total Multiplier (Ing CaCO 3 eL) (acceptable range (rag CaCOIL) (Ml) ml mi ml , 90 to 110%).033 40 50 .I.$. ms 2.0 A'S 40 10,Ot Duplicate sam ple precision: Sample Hardness %RPD-.Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) {(S D) /[(S+D)21} x 100 OSjarIL6M1I --yu 2. 66 MSS -1 4 1 A .4 11 S 6 Duplicate (B) T~~1.1 ~D S 4 .t Matrix spLke recovery: Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (rag CaCO3/L)(Ing CaCOtL) (ml) ml ml ml%los 10o3 4i0 SO mIq %C3 CA ,i .3o Sample hardness (B) Measured spike value (MV) % R = MV/ SV x100 (mg CaCOIL) MV = A -B (acceptable range (mug CaCO3/L) =75 to 125%)41+3

o.7.,o Sample measurements:_

Sample volume Begin End Total. Hardness Sample number Sample ED (mD ml ml ml Multiplier (mg CaCO3.L)RBank TV-__________ (should be =0m CaCO/L) ____________ _____~S.O .kZ. Y~IJU ISo U-.3 1411.0. 14A 1.6 _ _&__--- s_ _ 30.7 3At q&________ tI IA_ 30l 1.7 4 1.5 i.. r. A0. '4.1 _ _.3 -a.I___ _iNRage8lU d0trU 0Qused, sample must be diluted.Reviewed by: I j Aj Date reviewed 0%. a SOP C7 -Exhibit C7.1. revision 06-01 0 0 0 0~ mv on#y~1bslJngSciu2Ionz, ~nc.Page Page _3of Time initiated I ZZZ Time completed.s,l3.l'Total Hardness (SM 2340 C)RL =. 1.0 mg CaCO 3/L Analyst I3S Date analyzed 1 &S.t1. t-t I.Laboratory control standard: Reference standard 'True value Sample fHardness (MV) %RS=MV/TVxIOO number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (mg CaCO 3/L) (,ml) ml nml ml =90 to 110%)40 50 It.3 .ICSt 4o0z.o Duplicate s lm le precision: Sample Hardness %RPD =Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL) {(S -D) Ij(S+D)/2l} x 100 number (ml) ml ml ml , Duplicate (B) 4. ZL U5 4L V 0 Matrix spike recov , ..Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)(mR CaCOa/L) (ml) mi m_ mi_.....Mý 16 goSo TZ-LA &O'q (,* %q. t Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO 3/L) MV = A -B (acceptable range (rag CaCO3jL) =75 to 125%)Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3L)Rlnk "-"S -o--j_ _(should be = 0 ¶-4.iLAC,"r 3 S6 ,8.4 3?.'4 4.. , *i".. VA bI I M_, 1061_ 324 , 'U1- .0 60 6 _ _- 3Ic.. '40. ' 4.1 I1 __- _, # 4-7 4.) St 2 __ _____ ..0 ~410 41.4 ________E ~ z 3LS112 .14_ 13 4.1.sar dIA slOt I _ II % I , 7 ._&_ 87 l_____ 1LS S. 'IAý _____ ________Z_______ q 5in8jmbft~trfSis used, sample must be diluted. Reviewed by: IiII 1{ I Date reviewed l s -.SOP C7 -Exhibit C7. 1. revision 06-01-11 I Analyst 7 7 7 7 Date anaiyzed [~ K I Titrant no.-mality and muitinier determination: Page la Page 5_ of G Total Hardness (SM 2340 C)RL = 1.0 mg CaCOJL Time initiated Time completed Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference mlt ml ml = 0.2/E (N x 50000)/50 ml sample number number- acceptable range -0.0180 -0.0220) = Nx 1000 Laborato control standard:. Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (12g CaCO 3 IL) (acceptable range (mg CaCO0L) (ml) ml ml ml = 90 to 110 0/0)40 50 lC.' tW.i 2.0 Ii. £ 4o Duplicate sam le precision: Sample rdes% D=Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) I(S -D) /I(S+D)/2}) z 100 number _ (ml) ml ml ml , Duplicate (B) LIS ,., t ..D =' o t 42.Matrix spike recoyery : Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (tug CaCO 3/L)(Fag CaCOS/L (01l) ml ml ml~ ~ 4 .. *So I&.q zvItqy ~Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO 3 iL) MV -A -B (acceptable range I (Mig CaCO3/L) 7 to 2%)Sample measurements: Sample volume Begin End Total Hardness.Sample number Sample 111) (ml) ml ml ml Multiplier (tag CaCOVL)(shou-tdbe=0mgCaCO3/L) k%l.'..0%4 Wa 101 .,i yr- I so 1.-1g SO,_ 4.3 _q__"_I__ "B'.%i S 179 31. 2. 3 4-.S , q SA 4-94 WIC4 L .., -3,f z~f .q 77 I i Ra@s 8izn*Iitg is used, sampIe must be diluted., Reviewed by: I Date reviewed I os -l.SOP C7 -Exhibit C7. 1. revision 06-01-I1 ~~ ShY nmmn tuledaftns. Inc.Pape i Total Hardness (SM 2340 C)RL = 1.0 mg CaCO 3/L Page _ of 4 Time initiated Time completed 0&S IVl IM*Analyst Date analyzed rI~2 L~ilLJ I Labortory control standard: Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (ng CaCO3/L) (acceptable range (rg CaCOA/) (ml) ml ml ml -90 to 110%)4sicn0 50 12.4.7'Duplicate sam le precision: ....Sample Hardness %RPD Sample , Sample ID volume Begin End Total Multiplier (mg CaCO,/L) ((S -D) /[(S+D)/21} x 100 number (ml) m ml mi mspit.l qvv, Ih Lt ts %7- ZZ.4 0..1 .Duplicate (B) Dz L¶'.,=,- -,. ......- ..Matrix spike recovery: Reference standard Spike value Sample I Spike hardness (A)number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)(mg CaCO./L) (all) ml ml ml" io- 1033 S6 Z I,5 %.A Z.1 )Ito ,1lt _3___I (mg CaCO3/L) MV = A -B (acceptable range (mag CaCOs/L) = 75 to 125%)Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (Iog CaCO3L), 7'w .Blank ,,_'_"_"_ -°- l Z.._______ should be 0 m CaCO3./L) ,, ,,____sol 117 ?IL C *Z.'lI _ 57.v'l..m,12 7 ._.,______6 1,. Qz.C...A10.... 34I.- 3o.7 t.s ,,. ISO Vzaa ý4107 1AI______________ qo.7 SZ 4.5__,__l.,qLaSell,0446O used, sample must be diluted. Reviewed by: IDate reviewed[joj.,j,,.-, SOP C7 -Exhibit C7. 1. revision 06-01-11 1ýEnmbnMfttaI~esdng~o~uljmis, Ing, Page 11.-Page C of 6 Time initiated I Time completed -36 00It-i Total Hardness (SM 2340 C)RL = 1.0 mg CaCO 3/L Analyst I -.777 :77 Date analyzedI $.i, -tsr.I i i I 1 I I I Duplicate samle recson:[Sample Hardness %RPD =Sample Sample ID volume Begin End Total Multiplier (mag CaCO 3/L) [(S -D) /[(S+D)/21} x 100 number __ (m]) ml ml ml I[ D u p licate (B ) ... --1 --D" Matrix spike recovery: Reference standard Spike value Sample Spike hardness (A)number (SV) volume Begin End Total Multiplier (mg CaCO3/L)(ma CaCO 3 L) _(ml) ml ml ml Sample hardness (B) Measured spike value (MV) % R -V / SV x 100 (mg CaCOs/L) MV -A. -B (acceptable range S(mag CaCOVL) = 75 to 125%)Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (m]) ml ml ml Multiplier (mg CaCOAL)(should be = 0 ma CaCO 3/L) -*S.13.l" z'C6 a (."- 61% 11, l. S V,. z C1 7-M mt' 0.- FL .-C .. .z$.3 &.7 .,,__73___C1t1lifat is used, sample must be diluted. Reviewed by: ZZZ IZ Date reviewed o. r.'..., SOP C7 -Exhibit C7.1. revision 06-01-11 Sequoyah Nuclear Plant Biomonitoring May 08 -15, 2012 Appendix D Reference Toxicant Test and Control Chart Page 85 of 110 5hn Pimephales promelas@ Chronic Reference Toxicant-Control Chart o L T Organism Source: Aquatox, Inc.Environmental Testing Solutions, Inc.1.0 0.9 0.8 0.7 0.6 0.5 1.4 1.2 1.0.M 0.8; 0.6 0.4 I I I I I I I I I I I I I I I I I I i I USEPA Control Limits (+2 Standard Deviations) ..................... .......:.. .--..-.-...................... ..................................... I I I I I I I f I I I I I I I I I USEPA Warning and Control Limits (75th and 90th Percentile CVs).................................................. ........... .. .. .. ...... .. .. .. .. .. .. .. .. .. .. ... .. .. .. .. .... .... .. .. .. .. .. .... ..I.1.4 1.2 1.0 I I I I I I I I I I I I I I I I I I I 1 Laboratory Warning and Control Limits (10th and 25th Percentile CVs)I I I I I I I I I I

I I I. I i I -I " I I I 0.8 0.6 0.4 Test date 7-day IC25 = 25% inhibition concentration.

An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC 2 5)Warning Limits (mean IC 2 5 + SAM or SA.TS)Control Limits (mean IC 2 + SA.25, SA.90g or 2 Standard Deviations) Grephe piwterted from associated excel spreadsheet Excel spreadsheet ent ~d by; J. Summer Reviewed br.of" ..J5 1 TUFJ o Env~ronmental Testing Solutios., Inc Teat number Test date 7-day IC. CT (g/L K] (91 K)Pirnephales promelas Chronic Reference Toxicant Control Chart State and USEPA Laboratory S Control Umis SAM Warning ULmits CT-2S CTa2n a- SA. CT +SL S062S Control UmLits CT-s~ SA21CT "SAI USEPA SA" Wanning Limits CT-SA. C +Snt USWPA SLS Control UVaits C -SA.M CT+SMO CV 1 2 3 4 S 6 7 8 9 10 11 12 13 14 1i 16 17 18 19 20 03-29-11 03-31-11 05-03-11 05-07-11 06-14-11 07-12-11 07-19-11 0809-11 09-13-11 j0-4-1-1 10-11-11 10-18-11 fl-08-11 12-06-11 02-07-12 02-14-12 03-06-12 03-06-12 04-12-12 05-08-12 0.75`7S 0.78 0.76 0a63 0.67 0.63 0.67 0.66 a.73 0.76 0.67 0.78 0.77 0.77 0.74 0.64 0.65 0.71 0.77 0.72 0,72 0.72 0.72 0.71 0.71 0.71 0.71 0.71 0.71 0.71 0.71 an71 0.71 0.71 an71 0.71 0.71 0.71 0.72 0.04 0.04 0.04 0.04 0.04 0.04 0.05 0.05 0.05 0.05 0c05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.64 0.80 0.09 0.64 0.80 0.09 0.64 0.80 0`09 0.64 Mao 0a09 0`63 0.80 0.09 0.63 aso 0ace 0.62 0.80 0.09 0.62 0`80 0.09 Oi1 0.80 0.ac 0.61 118,1 0.09 0.62 0.81 0.09 0.61 0.81 0.09 0.621 0.2 0.09 0.61 0.82 0.09 0.61 0.81 0.09 0.61 0,82 0.09 0.61 0.82 0.09 0.61 0.82 0.09 0.61 0.82 0.09 0a61 0a82 0,09 0.63 0.6a 0.63 0.63 0.63 0.63 0.63 0.63 0.62 0.62 0.63 0.a3 0.63 0l63 0.63 0.63 0.63 0.63 0.63 0.63 0.81 0.80 0.8O 0.80 O.80 0.80 0-so 0.80 0.79 0.80 aso Mao 0.80 0.80 0.80 0.80 0.80 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15-0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.57 0.87 0.57 0.87 0.57 0.87 0.57 0.87 0.56 0.86 0.56 c.7 0.56 0.86 0.56 0.86 0.56 0.85 0.56 0.86 M56 0.86 0.56 .86 0.5 0.86 0.56 0.86 0a56 0.8a 0.56 0.86 0.56 0.85 0.56 0.86 0.6 0.86 0.57 0.87 0.27 0.27 0.27 0M27 0M27 0.27 0.27 0.27 0.27 M.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 OAS O.45 0.45 0.45 0.44 0.44 0.44 0.44.44 O044 0.44 0.44 0.44 0.44 0.44 0.44.A4 0.44 0.44 0.44 0.99 O.99 0.99 0.99 0.99 0.99 0.98 0.98 0.98 0.98 0.98 0.96 0.38 0.98 0.99 0.98 0.38 0.38 0.99 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0a32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.40 1,04 0.05 0.40 1.04 0.05 0.40 0.04 0.05 0.40 004 0.05 0.39 1.04 0.06 0.9 0.04 0.06 0.39 1.03 0.06 0.39 1.03 0.07 0.39 1.03 0.07 0.39 1.03 0.07 o.39 1.01 0.07 0.39 1.03 0.07 0.39 1.03 0.07 0.39 1.03 0.07 0.39 1.01 0.07 0.39 1.04 0.07 0.39 .103 0.07 0.39 1.03 c.07 0.39 1.03 0.07 0.39 1.04 0.08 Note: 7-d ICz 7-day 25% inhibition concentration. An estimation of the conmcntration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Cr = Central tendency (mean IC-).S = Standard deviation of the ICa values.Laboratoy Control and Warnlng UmIlts Laboratory control and warning limits were established usig the standard deviation of the C24 va lues corresponding to the loth and 25th percentila CVs. These ranges are more stringent than the control and wa ming limits recommended by USEPA for the test method and endpoint.SAta = Standard deviation corresponding to the percentile CV. (S5m = 0.12]5 Lm,= Standard deviation corresponding to the 25e percentle CV. (SAn = 0.21)USEPA Control and Warning Umits a Standard deviation corresponding to the 75h percentile CV. (S 5s = 0.38)SA.o- Standard deviation corresponding to the 90P percentile CV. (SnO= 0.45)CV = Coeffident of variation of the ICs values USEPA. 2000. Underrsndtn8 and Acnsriteantee Method Vrabhlisy In Winh. Fftluut Toxitity Applltons Ude thie National Polutant Dsdhsr 5 Ol.Mainaet Proeam, EPA-63-.R.004)0. US EnvonnmeMnal ProssOtceScenco, Cirldnnara, OH.File: ppklCr_050812.sx Entered by;. J. Sumner RevieW br. ... IT Environmental Testing Solutions, Inc.1,E , , Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source: Aquatox, Inc..-" 1.00 2 -0.75:0.50 U E 0.25 I I I I I I I III I I I I USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)I I I -I I I I I I I I I I I I I I I I 30.2.1., V , M. 0 o- Co 10 0 0 30 Kentucky Acceptance Limit (< 30.0%)....I~ ~ ~ ~~ I.I..I..................t t I a.20 I I I I I I i I I I I I I I I i I I I i USEPA Upper PMSD Bound (901h percentile < 30.0%)10 Test date-Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD)S~.Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) \iP9age 88 of 110 Graphs generaled from associated excel spreaddhest. Excel spreadsheet enter!tbY: J. Sumner Reviewed by: °F " Precision of Endpoint Measurements 0 T Pimephales promelas vhr.mth., lutoionie. Chronic Reference Toxicant Data Test number Test data Control Control Mean Survival Growth Cr Cv CT MSD PMSD CT for Control Growth (6) (mg/larvae) ((mg/larvae) for Control Growth CV (%)(N) for PMSD (%)1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 03-29-11 03-31-11 05-03-11 06-07-11 06-14-11 07-12-11 07-19-11 08-09-11 09-13-11 10-04-11 10-11-11 10-18-11 11-08-11 12-06-11 02-07-12 02-14-12 03-06-12 03-06-12 04-12-12 05-08-12 100 100 100 97.5 100 97.5 100 100 100 100 100 100 100 100 100 97.5 100 100 97.5 100 0.829 0.675 0.867 0.839 0.758 0.872 0.839 0.662 0.848 0,768 0.681 0.693 0.792 0.738 0.662 0.658 0.854 0.873 0.706 0.613 0.752 0.790 0.802 0.793 0.806 0.811 0.792 0.799 0.796 0.785 0.777 0.779 0.776 0.768 0.761 0.767 0.773 0.769 0.761 6.3 3.2 6.3 5.8 14.5 6.0 5.4 6,6 6.7 16.8 4.3 4.8 10.3 15.7 10.5 5.2 3.5 5.1 7.0 8.1 4.8 5.3 5.4 7.2 7.0 6.8 6.7 6.7 7.7 7.4 7.2 7.5 8.0 8.2 8.0 7.8 7.6 7.6 7.6 0.09 0.09 0.08 0.11 0.13 0.09 0.06 0.07 0.12 0.13 0.07 0.07 0.12 0.12 0.09 0.07 0.09 0.10 0.06 0.08 10.3 12.8 8.8 12.7 16.8 9.8 7.2 10.6 13.6 16.9 9.9 10.0 15.6 15.9 13.4 10.1 10.8 11.4 8.7 13.1 11.5 10.6 11.1 12.3 11.9 11.2 11.1 11.4 11.9 11.8 11.6 11.9 12.2 12.3 12.1 12.1 12.0 11.8 11.9 Note: CV -Coefficient of variation for control growth.Lower CV bound determined by USEPA (1 0' percentile) = 3.5%.Upper CV bound determined by USEPA (9&th percentile) = 20%MSD -Minimum Significant Difference PMSD -Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.Lower PMSD bound determined by USEPA (10'" percentile) -12%.Upper PMSD bound determined by USEPA (90lh percentile) = 30%.Cr = Central Tendancy (mean Control Growth, CV, or PMSD)USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.USEPA. 2001a, 2001b. Final Report: Intedaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-O1-005. US Environmental Protection Agency, Cincinnati, OH.I age 89 of 110 File: ppkclcr_050812.xlsx Entered by: J. Surwer Reviewed by: ETS Page I of 5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)Species: Pimephates promelas PpKCICR Test Number: Dilution epa ration in ormation: Comments.KCI Stock INSS number: luMA iw%.Stock preparation: 50 g KCI/L: Dissolve 0 g KCI in 1-L Milli-Q water.Dilution prep (rg/L) 7450 600 750 900 1050 " Stock volume (mnL) .9 12 15 18 21 Diluent volume (mL) 991 988 985 .982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism informnatio: -* Test information: Organism age: "MAQ 1% was OtI Randomizing template: 'g, j Date and times organisms % Wi.. &hmO Incubator number and were born between: shelf location: ._ _Organism source: A'M-A 6AMA 0.6 O+-t-L. Artemia CHM number: Cl'ti leST--." Drying information for weight determination: Transfer vessel pH S.U. Date / Time in oven: %ot! t i t'i l information: Temperature = .0 0C Initial oven temperature: 60 IL Average transfer volume: Date/Time out of oven; q6*n.-k .iI_4 Final oven temperature: 160"L4 Total drying time: J. 5-t .Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW or termination batch used Time Analyst Time Analyst Time Analyst--D.,0S. -s- I-L 2 Os to-ot~t1 _____ 14 4 %A: 0~ Ile~*1 S+'-2 Cototn m tion : ~ .......... ... Acetac riteri 4ummar oftsedon 3 M or. % < 4 0 ' o s.aL 0( r iý..1 02~d to ~ IqOO Ati I1 11li VA 7 OS,'- IL. ~r.Control information: Acceptance criteria Summary of test'end olnts.:% orait: 9200/ 7-daj LCso J ki 5L1_Average weit per initial larvae: 0. 16 PS NOEC Average weight per surviving larvae: j). it'S I t o. 25 mg/arfe LOE*"ea I ChV (010.8 IC2 l1q. S t-ge 90 of 110 SOP AT21 -Exhibit AT2 1.1, revision 06-01-11 I:0T Page 2of 5 Species: Pimephales promelas PpKCICR Test Number: Survival and Growth Data Day -Control 450 in KCI/L 600 mg KCI/L A "B' ,C D IE. F I G H I. J K L..-0 to -) -() o 0 to -co 10 1 .... 40 /0 , ' to t ' o/" 1 t o / to ,o /0 ,0 10 2 C/O t1o J1_ to , q ) /6 / o / o ' 1( /0 2 3 .,'/01 to.o o 0o 1o010 I) /10 /0 K) I (, ) 110 to It.o to. .o to .,0 to tIo ./0'o 'o 10 'o 10o ,,olQo eo , to ,,ot_ 1_ r3_ t( , It o t o /0 10 10 to i 1 t /0 I0 10 6_________ 10 o1 10 to 10, 10 /0/0 /0. 10 10 to-0D/b 10 16 10 lb' (0 It 1 t 0 /0, A -Pan weight (rag) 1 Tray color code:: V ., " "a4.ts 6C,.1. I' 14t ,1S.19 wL4, 13.3 1 13-97. S.,', I s.,4.' ,",.Pate: -o-41 B C Pan + Larvaeweight (m g)....C = Larvae weight (rag) = B -A'Hand calculated. 4.1O ',.S1 16-2(D 4s.d% 1 .51 1 $.I 1 s.gji 16-e U,15 16.11 7.l , Analyst, '. ---..I I I I I Weight per initial number of larvae (rag)= C Initial number of larvae Handcalcuyated. Analyst: .'a N'p-N 4%$0%08 Average weight per initial Percent number of larvae (rag) reduction from controlI ft fr...'-Sb,7. 0.b~ 4S !I-.Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.I ( ge 91 of 110 SOP AT21 -Exhibit AT21.1, revision 06-01-11 BET Page 3 of 5 Species: Pimephales promelas PpKCICR Test Number: LAk Survival and Growth Data Day 750 mg KCI/L 900 mi KCI/L 1050 ml KCIL M N 0 P Q R. S T U V W X o1( o , ,o Ao to '/D Traylo 77o.cd: --- -.. --A -Pan Irweight (m/g)(/ -AL ISM __so M l- 14.4Z 131Z 14. i .os t.,tt 1.4 4.94 1.Date: Date:_ __ aW4,7 Ile (a~ 'j 211- MP1-A1-,04 Hn c 3 BA I Aveagweight per initial Pero ent/onira m) ctia number of. "lar.vaeq.qZ O.0 ! a..Tra y colorlateo o : a Analyst: IWe '52 3'o0*i.Z~'.z 4oi.~i~j 34,.iI~BAvePane wegh par weigtia Percen nube oflarvae wih (mug) reuto B -A7 Had alulte.fromS q~ control 7~.S 11 3605 3.0~'i l .t 0.1 IOM Qommenat codes: c =clear, d =dead, f; = fungus, k = illed, m = missing, sk = sick, sm =unusually small, H g = unusually large, Mr = decanted and returned, w = wounded.I I I tComments: 92 of 110 SOP AT21 -Exhibit AT2 1.1, revision 06-01-11 CD*E TS Environmental Testing Solutions, Inc.Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number. 248 Test dates: May 08-15,21 ceninsrdten lrLlAXlj inedouhrefon Rnllm b Anper ll'slelt) 8 Pee slan. lanc.elglrte~l= Wl~gv~n kell/lllle.l~n~ lle Wntleclltll.ar~nlte ~d~ntel.1r IInrelberrbw l ~ 154l(l Mamve.Iu MlrVfd PecM r.&amlienfrau lane. neen A-s elaebnl~dhrse. le enu..slen..hmtensl plmWmlnant nsenbwe4,thne 1 corntrol (Il.'-t1041 (er1d A 10 10 14.68 2138 6.70 0.670 0.670 control B 10 10 13.56 .19.07 5.51 0.551 0.613 &.1 0i51 100.0 0.613 &.1 Notappllcable C -10 10 13.13 19.39 6.26 0.626 0.626 D 10 10 13.72 19.75 6.03 0.603 0.603 E 10 10 13.79 20.10 6.31 0.631 0.631 450 F 10 10 12-95 20.14 7.19 0.719 0.633 9.60X719 100.0 0.633 9.6 -3.3 6 10 10 13.39 19.36 5.97 0597 0.597 H 10 10 14.47 20.31 S.84 0.584 0.584 1 10 10 13.92 20.48 6.56 0.656 0.636 60 J 10 10 15.17 2136 6.19 0.619 0.651 6,8 0.619 1oo.o 0.651 6.8 -6.2 K 10 10 13.62 19.79 6.17 0.617 0.617 L 10 10 13.51 20.62 7.11 0.711 0.711 M 10 8 15.29 20.82 5.53 0.691 0.553 750 N 10 8 13.50 13.46 4.96 0.620 0.639 620A96 77.5 0.495 SA 19.2 0 10 a 13.74 18.47 4.73 0.591 06 75173 P 10 7 12.70 17.28 4.58 0.654 0.458 Q 10 4 14.62 18.04 3.42 0.355 0.342 9R R _ 10 5 13.32 16.82 3.50 0.700 0.773 8.3 0.350 47.5 07365 6.3 40 S 10 5 14.05 17.83 3.78 0.756 0.378 T 10 5 14.05 17.96 3.91 0.782 0.391 U 10 1 13.81 14.64 0.73 0.730 0.073 1050 -10 1 13.44 1428 0.84 0.840 0.813 14.7 0.084- 10.0 0.081 14.7 86.7 W 10 1 14.89 15.60 0.71 0.710 0.071 X_ _ 10 1 1429 15.26 0.97 0.970 0.097 Ourrett's MSO value: PMSD: 0.0800 13.1 MSD = Minimum Silnnificant Difference PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD Is the minimum percent difference between the control and treatment that can be declared statistically significant In a whole effluent toxicity test.Lower PMSD bound determined by USEPA (10th percentile) = 12%.Upper PMSD bound determined by USEPA (90th percentile) = 30%.Lower and upper PMSD bounds were determined from the loth and 90th percentile, respectively, of PMSD date from EPA's WET Interlahoratory Variability Study (USEPA, 20016; USEPA, 2001b).USEPA. 2001a, 2001b. Final Report; Interiaboratory Variability Study of EPA Short-term Chrmnlc and Acute Whole EffluentToxlcfty Test Methods, Volumes I and 2-Appendbi. EPA-821-8-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Chrclnnati, OH.File: ppkclcrO05012.xsl Entered by: J Synrer lkesrewed by..X EFTS Statistical Analyses Larval Faih Growth and Survival Test-7 Day Survival Start Date: 5/8/2012 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 5/16/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Plmephales prometas Comments: Cono-mg/L 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 600 1.0000 1.0000 1.0000 1.0000 760 0.8000 0.8000 0.8000 0.7000 900 0.4000 0.5000 0.5000 0.5000 1050 0.1000 0.1000 0.1000 0.1000 Transform: Arcain Square Root Rank 1-Tailed Number Total Mean N-Mean Mean Min Max CV% N Sum Critical Reasp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 460 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 800 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40*750 0.7760 0.7750 1.0782 0.9912 1.1071 5.379 .4 10.00 10.00 9 40*900 0.4750 0.4750 0.7802 0.8847 0.7854 8.622 4 10.00 10.00 21 40"1050 0.1000 0.1000 0.3218 0.3218 0.3218 0.000 4 10.00 10.00 38 40 Auxiliary Tests Statistic Critical Skew Kurt Shapim-Wilkes Test indicates non-normal distribution (p <= 0.01) 0.63088 0.884 -2.1517 5.4494 Equalit of variance cannot be confirmed Hypothesis Test (14all, 0.06) NOEC LOEC ChV TU Steel's Many-One Rank Test 800 750 e70.82 Treatments vs D-Control Maximum Llkellhood-Problt Parameter Value SE 95% FiducIal Limits Control Chl.q Critical P-value Mu sigma Iter Slope 14.4814 1.07334 10.6137 18.3492 0 1.78643 7.81472 0.61789 2.93943 0.06905 3 Intercept -37.607 .8.0048 -48.93N -20.108 TSCR 1.0 Point Problits mglL 96% Fiduclal Limits 0.9 ECOI 2.674 600.876 521.355 654.429 EC05 3.355 669.845 602.697 714.916 0.8 EC10 3.718 709.467 850.478 750.158 0.7 EC15 3.964 737.664 684.352 775.454 EC20 4.158 760.871 712.091 79a.859 o0.6 EC25 4.326 781.362 738.336 815.805 10.5 EC40 4.747 835.476 798.142 869.388 ECS0 6.000 869.818 834.745 908.603 10.4 EC0O 6.253 905.673 870.247 948.43 0.3 EC75 5.674 968.289 927.107 1028.37 0.2 EC80 5.842 994.365 949.312 1003.47 EC85 8.038 1025.65 975.202 1100.85 0.1 ECO0 6.282 1008.41 1008.03 1164.38 0.0 ..EC95 8.840 1129.83 1057.85 1256.74 1 10 100 1000 10000 EC99 7.326 1259.13 1155.35 1452.86 Dose-Response Plot I* Page 94 of 110 File: ppkclcr_00812.xlsx Entered by: 3. Siner Reviewed by. S S 0ETS Statistical Analyses Larval FPh, Start Date: 515/2012 Test ID: PpKCICR End Date: 6/1&12012 Lab ID: ETS-Envi Sample Date: Protocol: FWCHR-4 Comments: Cone-mg/L 1 2 3 4 D-Contral 0.670o 0.6610 0.6280 0.o030 450 0.6310 0.7190 0.5970 0.6840 600 0.5560 0.8190 0.6170 0.7110 750 0.8530 0.4980 0.4730 0.4580 900 0.3420 0.3500 0.3780 0.3910 1050 0.0730 0.0840 0.0710 0.0970 Growth and Survival Test-7 Day Growth Sample ID: REF-Ref Toxicant r. Testing Sol. Sample Type: KCL-Potassium chloride EPA-821-R-02-01 3 Test Species: PP-Pimephales promelas Transfomrm Untransformed 1-Tailed Isotonic Coscn-mu/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6125 1.0000 0.8125 0.5510 0.6700 8.087 4 0.6320 1.0000 450 0.6328 1.0331 0.6328 0.5840 0.7190 9.812 4 -0.552 2.180 0.0800 0.8320 1.0000 600 0,8508 1.0624 0.6508 0.6170 0.7110 6.760 4 -1.042 2.180 0.0800 0.6320 1.0000 760 0.4950 0.8082 0.4950 0.4580 0.6530 8.425 4 0.4950 0.7832 900 0.3853 0.5963 0.3653 0.3420 0.3910 6.320 4 0.3653 0.5779 1050 0.0813 0.1327 0.0813 0.0710 0.0970 14.714 4 0.0813 0.1286 Auxiliary Tests statistic critiCal SkeW Kurt Shaplro-Wilk's Test Indicates normal distribution (p > 0.01) 0.92935 0.805 0.61878 -0.8846 BEartlett's Test Indicates equal variances (p = 0.ff7 0.28594 9.21035 Hypothesis Test (1-tail. 0.06) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetrs Test 600 >600 0.08004 0.13067 0.00146 0.0027 0.89872 2,9 Treatments vs D-Contol Unear Interpolation (200 Resamples) Point m g/L SD 98% CL(Exp) Skew&C05 834.60 28.01 593.31 661.72 -7.8928 s10 689.20 14.88 633.19 723.43 0.9209 ICiS 703.80 19.42 68W.12 781.73 0.9450 1.0 IC20 738.39 20.48 6894.18 806.57 0.4813 0.9 IC25 774.28 20.26 718.07 838.43 0.1632 0.8 IC40 883.07 12.92 841.40 918.61 -0.1823 0.7 IC-50 92601 672 90842 943 1/ -0 07950.1 0.6.j 05!0.4 i0.3 0.2-0.1 0.0 4-0.1*Dose.espone Plo 500 1000 1500 Dose mgIL Dose-Reeponee Plot 0.7 0.6!0.5 04 0.3: 0,2-0.11 b )1-tall, 0.06 level of signflicance (iL age 95 of 110 Fi&c ppkclcr_05092.xIsx Entered by- J. Susrn Reviewed by k Page 4of 5 Species: Pimephales promelas PpKCICR Test Number: Daily Chemistir D: 0 2________Analyst IC_ _ _ _ _ _ _ ____ __ _Concentration Parameter Conductivity (iimhos/cm) Ul 0 CONTROL Alkalinity16.fiID CONTROL CaCOL Hardness 5 Temperature , CpH (S.U.) IAZAGj ___Do p~~__ ...._____ , ..450 mug KCVL Conductivity Temperature "L- 1 $. "1. A "1-'] .1 6 Conductivity 450 ig KCIL ughos/cm) .10 1040 Temperature -NI " -~-D 750 mg KCI/L Conductivity

age 96 of 110 I Final I -nitial I Final 0 1 SOP AT21 -Exhibit AT2 1. 1, revision 06-0 1 -1 j U 0 3 Page 5 of 5 I Species: Pimephales promelas PpKCICR Test Number: I I I I I U r Day 3 4 5 6 Analyst 4 -II k.Aall, II 14wi, I AUj- I 'LV' _ ~ j IfLL An I -I Concentration I Parameter_____

I-~pH (S.U.)71.910" ir.si -4 1 aI 11 14M 1~ I40,1 11 q ~ BI WO " Conductivity .3z34 (1.3 (mhos/cni) CONTROL Alkalinity ..ii (mug CaCO 3/L) 1 Hardness 81 (mg CaCO 3/L)Temperature I -a VV'l A ". S L. '4.1 PH(. 2.) -46' ---450 mg KCIL Conductivity 4 K mhos/cm) 0 O t I loo Temperature (0 C)VIA ý 14.2- 1 T-I.-t 11 IVA.-L I -Lq..l I -LIA--L- I _L4'1 ý M-71 1 01- ,---.---d ..-a.-i -SmIl pf (S.U.)I 600 mg KCI/L DO (mg/L)Conductivity Temperature1 6'(1 .14 (1 865 i I I I I DO (mg/L) 13 (SU. 19.2 --4 750 mg KCI/L Conductivity I,0IwX 16eO (ipmhos/cm)."t~ .1 P, "

1.3 2'L. l ... -...--%q l-900 mg KCI/L Conductivity Ie~Temperature Pf(S.U.) , .61q 4.q.3 DO (sg/L) ,,4 W, qq To 1050 rug KCIIL Conductivity I8I Amhogscm 2O~ (1860100o Temperature 41Z.1 ' 4 " 9.Temperature TA" 1 2(.- -,- -.-, Initial H Final I Initial I Final I Initial II Final I Initial I Final I-II -h I I -I I I I1 I age 97 of 110 SOP AT221 -Exhibit AT2 1.1, revision 06-01-11 n Environmental Testing Solutions, Inc.Ceriodaphnia dubia Chronic Reference Toxicant Control Chart 1.14 1.12 1.10 1.08 1.06 1.04 1.02 I I I I I I I I I I I I I I I I I I I I USEPA Control Limits (+/-2 Standard Deviations)

I I I I II I I I I I I I I I I I I I I 2.01.5 U 1.0 , 0.5 I, I I I I I I I I I h .I th I I I I I USEPA Warning and Control Limits (75h and 90 Percentile CVs)........... ...... ............. ......I f I I II I I I I I I I I I I I I 1.4 1.3 1.2 1.1 SI I II I I I I I I I I I I I I I I I I" Laboratory Warning and Control Limits (10th and 2 5 th Percentile CVs).... ................................................ ............................................. f... ......... .............. 1.0 0.9 0.8*ýN V- N**- ýN ISN NN NN NN NN NN -ýN INA- NN N$ 0 'VI S1 ový 6", oil," 00 00, *11', ;0, ;'VO, aow 0,ýý o:%P-Test date-7-day IC 2 5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean IC 2 s)Warning Limits (mean IC25 +/- SSA.10 or SA.75)... Control Limits (mean IC 2 5+/- SA 2 , SA.90, or 2 Standard Deviations) DPage 98 of 110 Graphs generated from associated anasl spreadshieet. Excel spreadsheet entered by: J. Suinner Reviewed br. CD S (0 Ceriodaphnia dubla O -- Reference Toxicant Control Chart Environmental Testing Solutions, Inc.Test number Test date 7-day IC, CT (9/L NaCi) (/L NaC1)1 01-18-11 1.06 1.07 2 02-08-11 106 1.07 3 03-,08-11 1.05 1.07 4 04-05-11 1.06 1.07 5 04-05-11 1.07 1.07 6 05-03-11 1.07 1.07 7 06-07-11 1.05 1.07 8 07-12-11 1.06 1.07 9 08-09-11 1.06 '107 10 09-13-11 105 1.07 11 10-04-11 1.07 1.07 12 10-11-11 1.06 1.07 13 11-08-11 1.05 1.07 14 12-06-11 1.06 1.06 15 01-10-12 1.08 1.07 16 02-07-12 1.08 1.07 17 03-13-12 1.0 1.07 18 04-10-12 1.07 1.06 19 04-10-12 1.07 1.06 20 05-08-12 1.06 1.06 State and USEPA Laboratory S Contbrl Limits SAM Wanting Umits SA Cr-2s Cr+2s cr-.s, CT + Sx.Laboratory Control Unit CT -SAM CT" +SAM USEPA SLS Warning Limits CTr- s,.n USEPA SAM Control Limits Ct- S". Cr+Su CV 0.01 1.05 1.10 0.09 0.99 0.01 1.05 1.09 0.09 0.9 0-01 1.05 1.10 0.09 0.99 0.01 1.05 1.09 0.09 0.98 0.01 1.05 1.09 0.09 0.93 0.01 1.05 1.09 0.09 0.98 0.01 1.05 1.09 0.09 0.98 0.01 1.04 1.09 0.09 0.98 0.01 1.04 1.09 0.09 0.98 0.01 1.04 1.09 0.09 0.92 0.01 1.04 1.09 0.09 0.98 0.01 1.05 1.08 0.09 0.98 0.01 1.05 1.08 0.09 0.98 0.01 1.04 1.08 0.06 0.98 0,01 i,4 19 0.09 o o0a 0.01 1.04 1.09 0.09 0.98 0.01 1.04 1.09 0.09 0.98 0.01 1.05 1.08 0.09 0.98 0.01 1-05 1.08 0.09 0.98 0.01 1.05 1.0O 0.09 0.98 1.16 0.18 0.89 1.16 0.18 0.89 1.16 0.18 089 1.16 0.18 0.89 1.16 0.18 0.89 1.16 0.18 0.89 1.15 0.18 0.89 1.15 0.18 0.89 1.15 0.18 0.89 1.15 0.18 0.89 1.15 0.18 0.89 1.15 0.18 0.88 1.15 0.18 0.88 1.15 *0.18 0.88 1.15 0.18 018 1.15 0.18 0.88 1.15 0.18 0.88 1.15 0.18 0.88 1.15 0.18 0.88 1.15 0.18 0.88 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0A8 0-59 1.25 0AS 0.59 1.25 0.48 0.59 1.25 OAS 0.59 1.25 0.48 0-59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 A8 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 '0.59 124 0.48 0.59 1.24 0.48 0.59 1.56 0.66 0.41 1.55 0.66 0.41 1.55 0.66 0.41 1.55 0.66 0.41 1.55 0.56 0.41 1.55 0.65 0.41 1.55 0.66 O.41 1.55 0.66 0.41 1.55 0.66 0.41 1.55 0.66 0.41 1.55 0.66 0.41 1.55 0.66 0,40 1.54 0.66 0.40 1.54 0.66 0.40 154 0a6 0,40 1.55 0.66 0.40 1.55 0.66 0.40 1.54 0.66 0.40 1-54 0.66 0.40 1.54 0.66 0.40 L74 0.01 1.74 0.0o 1.74 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 o0 1.73 o.01 1.72 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.72 0.01 1.72 0.01 1.72 0.01 Note: 7,-d IC2 = 7-day 2536 inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodophnio reproduction forthe test population. CT = Central tendency (mean IC2).S = Standard deviation of the IC2a values.Laboratory Control and Waming IUmits Laboratory control and warning limits were established using the standard deviation of the IC, values corresponding to the loth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.= Standard deviation corresponding to the 10e percentile CV. (5&A1 = 0.08)SeAs -Standard deviation corresponding to the 2 5 V percentile CV. (S5m = 0.17)USEPA Control and Warning UmIts 8 =76 Standard deviation corresponding to the is percentile CV. (Su.: = 045)Sf= Standard deviation corresponding to the 90" percentile CV. (S"D = 0.62)CV = Coefficient of variation of the IC,, values.USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxic Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.Fie: CdNaClCR_0t082td~sx ReviMad b Jt. 0') Environmental Testing Solutions, Inc-Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements V 0 T 04 is Is 40 35 30 25 20 15 M I I I I i I I I I I I I i I i I I I I 1 -Minimum Acceptance Criteria (> 15.0 offspring per surviving female)-I I I I I I I I I I I I I I I I 40* ! ,30* -20"" 10 50 40 N, 30 X 20 10 0 i, I I I I I I I I I I I 1 I I I I I I North Carolina Acceptance Limit (< 40.0%)Kentucky Acceptance Limit (< 30.0%)!!.. ... .... .... .. .. .. ............ "'l. ................. ! ... !....... ....i~~. ............................. ,,I I i-I I I I I I I I _ _ I _ _ I I _ _ I __ I I I I I I I USEPA Upper PMSD Bound (9 0 t percentile < 47.0%)I ,I I I. I I I I I I I I '" I I I I I I I I " SN AN AN AN '8N SN SN AN -o Test date---- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD).Control Limits (mean Control Reproduction, CV, or PMSD :L 2 Standard Deviations) age 100 of 110 Graphs generated from assodated excel spreadsteet. Excelsireadsh d t' erhred by: .. Sumner Reviewed by: .I I .Environmental Testing Solutions, Inc.Precision of Endpoint Measurements Ceriodaphnla dubia Chronic Reference Toxicant Data Test number Tsdae Control Control Mean Survival Reproduction (%) (offspring/female) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 01-18-11 02-08-11 03-08-11 04-05-11 04-05-11 05-03-11 06-07-11 07-12-11 08-09-11 09-13-11 10-04-11 10-11-11 11-08-11 12-06-11 01-10-12 02-07-12 03-13-12 04-10-12 04-10-12 05-08-12 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 31.9 30.7 32.6 32.8 33.0 30.9 28.9 32.2 28.3 32.1 32.8 31.3 29.0 29.3 32.4 31.7 31.1 29.4 30.3 31.7 Cr for Control Mean Reproduction (offspring/female) 31.3 31.7 32.0 32.2 32.0 31.5 31.6 31.3.31.3 31.5 31.5.31.3 31.1 31*2 31.2 31.2 31.1 31.1 31.1 CV CT for Control (%) Reproduction CV (%)MSD PMSD CT (%) for PMSD(%)4.8 6.3 6.9 4.8 5.9 4.5 6.7 6.0 5.0 8.2 6.1 3.0 7.6 5.8 5.1 4.2 7.2 6.8 7.1 6.1 5.6 6.0 5.7 5.8 5,5 5.7 5.7 5.7 5,9 5.9 5.7 5.8 5.8 5.8 5.7 5.8 5.8 5.9 5.9 2.0 2.3 1.8 2.0 2.2 1.9 2.5 2.0 1.9 1.9 2.1 2.1 1.8 1.9 2.4 2.1 1.9 2.4 2.7 1.9 6.3 7.4 5.4 6.0 6.7 6.1 8.7 6.2 6.8 5.8 6.5 6.6 6.1 6.5 7.4 6.5 6.1 8.0 8.8 6.1 6.9 6.4 6.3 6.4 6.3 6.6 6.6 6.6 6.5 6.5 6.5 6.5 6.5 6.6 6.6 6.5 6.6 6.7 6.7 Note: CV = Coefficient of variation for control reproduction. Lower CV bound determined by USEPA (1 0 th percentile) = 8.9%.Upper CV bound determined by USEPA (9 0 1h percentile) = 42%MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statisticallv siRnificant In a whole effluent toxicitv test.Lower PMSD bound determined by USEPA (1 0 b percentile) 13%.Upper PMSD bound determined by USEPA (90 th percentile) 47%.CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD)USEPA. 2000. Understanding and Accounting for Method Variability In Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cncinnati, OH.USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.101 of 110 File: CdNaCICR_0508 12.xlsx Entered by: J. Sumner Reviewed by: F0T Page 1 of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)Species: Ceriodaphnia dubia CdNaCICR #: Qt Dilution preparation information: Comments: NaC! Stock INSS number: I4 $0_a Stock preparation: 100 g NaC1/L: Dissolve 50 g NaC! in 500 mL Milli-Q water.Dilution prep (rmgL) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 1 10Total volume (mL) 15 .1500 1500 1500 1500 1 Test organism source information: Test information:. raism age:, < 24-hours old Randomizing template color. .Date and times organisms were born V.0' I-- 11 0 & tA 0M CU Incubator number and shelf between: .location: Culturb board: M-11. t7-. IA Replicate numbr 2 1 3 1 4 5 7 I 9 10 YWTbatch: Cultureboardo cnumber: I 101U. X_ _%51n1U _S._ 0 Transfer vessel information: pH = % S, S.U. Temperature-= OC Selenasirm batch: Average transfer volume (mL: 0. _"______________- Daily renewal information: Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0 Qsl~ _ _ _ _ __ _ '-o 4&2 os "I0io'r. ti) tL-_ _ O. It- IrL 0& 10 L I 7 -I OW) Il 1O m A. ...OL.. , 6 a'l-u 80as Control Information: Acceptance criteria ya of test endpoints: % of Male Adults: ., .20% 7-day LCsq _ _1__00% Adults having 3'r Broods: T 2:- 80% NOEC 1O=% Mortality:

01. <20% LOEC Mean Offspring/Female:

2t 15.0 offspring/femaie .ChV I% CV: < 40.0 % I C25 1011.4 f Page 102 of 110 SOP AT 14 -Exhibit AT 14.1, revision 06-01 -11 F. TS Species: Cerlodayknia dubia 1 CONTROL Page2 of 6 CdNaCICR #: tt't Survival and Reproduction Data:3 Replicate number Day "_ 1 2 3 4 5 6 7 8 9 10 1 -on produ-ed Adult mortality L. L. L- =1 Young produced n 1 ... C 0 ....Adult mortality U ., 2 Young produced .0 C..Adult mortality %- U. k--4 Young produced " 1 05 Adult mortality Young produced Adult mortalit C-6 Young produced 0". lL L' 1A IS l' I t I i0 %L.C Adult mortality 7 Young produced It.f., i I to. tj Total young produced " 3 Final Adult Mortality t U .. C? -.1j -1 .._. -- 7 X for 3V Broods I X- I y >2. I % .Note: Adult mortality (L -live, D -dead), SB -split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer). Concentration: I/o Mortality: I l7., Mean Offspring/Female: 600 m NaCI/L Survival and Reproduction Data Replicate number Day J 1 2 3 4 5 .6 7 8 9 10 1 Young produced -I[, Adult mortality %. , I I L L i( .2 I Young produced 0 0 1 Adult mortality .. U _3 Young produced C) 0 0 o I ..Adult mortality J , _ -U.. U .II 5 Young produced I"_____ ..l I Adult mortali , , Adult mortality ' _ _-" -6 Young produced -0 _c 6 Adult mortality e Li 7 1Young produced] J, Is ~ ~ ,j I L 16.Total young produced &f 57 46 7A 3jZ2. 34 :& 30 W4 3 Final Adult Mortlt 'I ~? U ~ ~ *? c: c C Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offtipring carried over with adult durine transfer). Concentration: Mean Offitpring'Female: 1 1 ."ll* age 103 of 110 VA Reduction from Control: I (0".0 L SOP AT14 -Exhibit AT!4.1, revision 06-01-11 F 5 Page 3 of 6 Species: Ceriodaphnia dubia 800 lg NaCI/L CdNaCICR #M Survival and Reproduction Data..... ___Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced £ .cJ J Adult mortality

j. _2 Young produced 0*Q 10 * ~_f Adult mortality I -.L- L 3I Young produced Adult mortality

-72J -- -I ,,7 -.. L..Young produced S C_Adult mortality %[-- % .4 4 L_S Young produced "L___ ___Total young produced B *L& -6 L I- ~.3 -L- 3 5 Note: Adult mortality (L -live, D -dead), SB -split brood (single brood split between two days). CO = carry over (offspring carried over with adult during transfer). Concentration: %Mortality: 0].I1Mean Offspring/Female: 31.1A. I% Reduction from Control: 1.117-1000 mg NaCI/L Survival and Reproduction Data Replicate number Day. 1 2 1 3 1 4 5 6 7 a 9 10 I Young produced F _5 Q L .Adult mortality __ L L L- L, i 4 _2 Young produced £flV l 0 10 Q Adult mortality I._- I L.- L_ -_3 Young produced _ _ --Adult mortality = .....R -,, L_4 Young produced _ _ q .t_- S Adult mortality _ _'6 Young produced I ~ -'- [-'L I-~ 10-Adl motlt t= -_ t= = =6 Young produced CL 0 6 0 I 6 I Adult mortality %_ __. ____[ L.t." ..- '- I.-_J 7 Young produced %4--16 i '(1 11f I Is I la Is Total young produced 11 130 1 at) 132- 111 11 1 6 131 17- 2 1 'SO t Mortality I _ _l __ I_,__ I__ i _ 1 %-Ik- I %-.1 X Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer). Concentration: % Mortality: Mean Offspring/Female: 30.2-S% Reduction from Control: q. &SOP ATI4 -Exhibit AT14.1, revision 06-01-11 , Page 104 of 110 qI Page4o;6 4 f , I] Species: Ceriodaphnia dubia 1200 mg NaCI/L CdNaCICR #: Survival and Reproduction Data I].......... __Replicate number Day 1 2 3 4 5 6 7 8 9 10 Youngprodued 0 0 C Z --..Adult mortality l .[ L.-C X- C- C -C1 ]--_Adult mortality __ C7 3 Young produced o) 0 0 0. C C.. I).. .4 Young produced CI Adult mortality _.[ _ .[ L t.. L "-5 Young produced Adult mnortality~~____ -' --'6 Young producedJ0 0 0 cc) 1,1 o__-fAdult mortalit \-_ \-- %.- t,.ý %- ~ l I.- t 7 Youngproduced T -& r T 'Totalyoungproduecd 1 eo 1L 1 l I .3 l Z. " 1 & I Final Adult Mortality j ' ( '.- \ L. -.j T Note: Adult mortality (L = live, D -dead), SB = split brood (single brood spilt between two days), CO = canry over (onsprig carried over with adult during translr).Concentration: % Mortality: 07, Mean Offspring/Female: -'I% Reduction from Control: A. 1 Survival and Reproduction Data 1400 mg NaCI/L[ Replicate number Day _ 1 2 3 4 5 6 7 8 9 10 I Y o u n g p r o d u i e d r e) e) ne i t Adult mortality ____ ._.- __-_ ._.__,__ ,.Adult mortality [- , l 2 Young produced 0- C C) 0 1 C) C) >Adul mo lit C o ro CoCL-o 3 Young produced 0 )100 0 10__ Adult mortality I _ L4 _ L. L L. L _ _4 Young produced[~~C 0 1I 0 C), ~ I C.~I.~Adult mortality 6 Young produced C) (Adult mortality L- l I 1 7 Young produced S " Totalyoungproduced SI 4 ... I 3 '1'4- I 4 Aumrlity -k.l ----Final Adult Mortality C- %-- C .- %- U-- I %-.T Note. Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days). CO = carry over (offspring carried over with adult during transfer). J Concentration: %.Mortality: I 17_Mean OffsprinR/Fernale: I S* Page 105 of 110 I % Reduction from Control: I M.7 SOP ATI4 -Exhibit ATI4. 1, revision 06-01-i l °or EnvironmentalTesting Solutlns, Inc.Control'7 Verification of Ceriodaphnia Reproduction Totals Re Plicate number Total Day 1 2 3 4 5 6 7 8 9Toa 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 "0 0 0 0 0 0 0 4 5 4 4 5 6 5 5 4 4 4 46 5 12 12 10 .11 10 11 10 11 11 12 110 6 0 0 0 0 0 0 0 0 0 0 0 7 17 17 15 13 15 18 15 18 16 17 161 Total 34 33 29 29 31 34 30 33 31 33 317 600mgN n Re plicate number Total-Y 1 2 3 4 5 16 17 8 9 110 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 4 5 4 4 6 4 44 5 1212 10 10 10 12 11 10 12 11 110 6 0 0 0 0 0 0 0 0 0 0 0 7 17 16 15 15 18 17 18 16 .16 15 163 Total 33 32 30 29 32 34 33 30 34 30 317 800 mng NaCII __ _ _ _Day Replicate number Dy 1 2 13 4 1 5 6 7 8 9 10 Total 1 0 0 0 00 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 4 5 5 4 4 5 4 4 45 5 10 11 10 13 11 12 10 10 12 10 109 6 0 0 0 0 0 0 0 0 0 0 0 7 18 16 14 14 16 15 13 18 16 17 157 Total 33 32 28 32 2 31 27 33 32 31 311 1000 mn NaCI/L Day Replicate number Total Dy 1 2 .....3 14 5 6 17 18 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 T 0 00 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 4 4 4 4 5 5 43 5 12 10 11 13 11 10 12 12 10 10 111 6 0 0 0 0 000 0 0 0 0 7 15 16 4 15 16 13 17 15 1315 149 Total 31 30 30 3231 27 33 31 28 30 303 1200 m NaCIIL Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0- 0 0 4 0 1 1 2 1 3. 3 1 1 2 15 5 3 5 2 3 3 0 0 2 4 1 23 6 0 0 0 0 0 5 2 0 0 0 7 7 7 6 4 6 5 5 7 4 3 5 52 Total 10 12 7 11 9 13 12 7 8 8 97 1400m NaCIL/L" Re klicate number Told 1 2 3 4 5 6 7 0 9 10 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 01 0 0 0 0 b 0 0 0 0 3 0 0 0 0- 0 0 0 0 0 0 0 4 0 0 1 0 1 0 1 1 0 0 4 5 0 1 0 1 0 2 0 0 3 2 9 6 0 0 0 0 0 0 0 0 0 0 0 7 5 3 0 3 4 1 3 0 0 2 21 Total 5 4 1 4 5 3 4 1 3 4 34 File: CdNaCICR_050812.xlsx Entered by: J. Sumner Reviewved by:._ CD 04 Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc.Test number.CdNaCICR #134 Test dates: May 08-15, 2012 Concentration Replicate number Survival Average reproduction Coefficietof Percent redction from (mg/L NaCI) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) varlatia (%) control (6)Control 34 33 29 29 31 34 30 33 31 33 100 31.7 6.1 Not applicable 600 33 32 30 29 32 34 33 30 34 30 100 31.7 5.8 0.0 800 33 32 28 32 32 31 27 33 32 31 100 31.1 6.5 1.9 1000 31 30 30 32 31 27 33 31 28 30 100 30.3 5.8 4.4 1200 10 12 7 11 9 13 12 7 8 8 100 .9.7 22.8 69.4 1400 5 4 I 4 5 3 4 1 3 4 100 3.4 42.1 89.3 Dunnett's MSD value: PMSD: 1.927 6.1 MSD= Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test Lower PMSD bound determined by USEPA (1 0& percentile) = 13%.Upper PMSD bound determined by USEPA (9 0'h percentile) = 47%.Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Intetlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).USEPA. 2001a,2001b. Final Report: Intedaboratory Variabilily Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01 -005.US Environmental Protection Agency, Cincinnati, OH.File: CdNaCICR_050812.xlsx Table populated from associated 'Verification of Coiodaphnia Reproduction Totals" spreadsheet. Spreadsheet entered by:. J. Sumner Reviewed by: __f V 0 0ET E Statistical Analyses Start Date: 5/812012 End Date: 5/1512012 Sample Date: Cnmmontu.- Carlodaphnla Survival and Reproduction Test-Reproduction Test ID: CdNaCICR Sample ID: REF-Ref Toxicant Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodlum chloride Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Conc-mgfL 1 2 3 4 5 6 7 8 9 10 D-Control 34.000 33.000 29.000 29.000 31.000 34.000 30.000 33.000 31.000 33.000 600 33.000 32.000 30.000 29.000 32.000 34.000 33.000 30.000 34.000 30.000 800 33.000 32.000 28.000 32.000 32.000 31.000 27.000 33.000 32.000 31.000 1000 31.000 30.000 30.000 32.000 31.000 27.000 33.000 31.000 28.000 30.000 1200 10.000 12.000 7.000 11.000 9.000 13.000 12.000 7.000 8.000' 8.000 1400 5.000 4.000 1.000 4.000 5.000 3.000 4.000 1.000 3.000 4.000 Transform: Untransformed 1-Tailed isotonic Conc-ma/L Mean N-Mean Mean MIn Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 31.700 1.0000 31.700 29.000 34.000 6.140 10 31.700 1.0000 600 31.700 1.0000 31.700 29.000 34.000 5.789 10 0.000 2.287 1.927 31.700 1.0000 800 31.100 0.9811 31.100 27.000 33.000 6.511 10 0.712 2.287 1.927 31.100 0.9811 1000 30.300 0.9558 30.300 27.000 33.000 5.832 10 1.161 2.287 1.927 30.300 0.9558'1200 9.700 0.3060 9.700 7.000 13.000 22.821 10 26.109 2.287 1.927 9.700 0.3060*1400 3.400 0.1073 3.400 1.000 5.000 42.054 10 33.586 2.287 1.927 3.400 0.1073 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 1.02293 1.035 -0.3894 -0.8258 Bartlett's Test Indicates egual variances (p -0.88) 1.79808 15.0863 Hypothesis Test (1-tall, 0.051 NOEC LOEC ChV TU M1Du MSDP MSB MSE .F-Prob df Dunnets Test 1000 1200 1095.45 1.92678 0.06078 1662.68 3.55 3.7E-43 5, 54 Treatrmnts vs D-Control Linear Interpolation (200 Resamiies) Point malL SD 96% CL Skew IC05 1001.8 91.4611 703.535 1009.92 -1.5251 IC10 1017.18 16.9162 1002.53 1024.94-11.3848 ICIS 1032.57 5.71887 1018.68 1040.35 -0.2299 IC20 1047.96 5.47513 1034.69 1055.49 -0.1685 0.9 IC25 1083.35 5.29693 1050.98 1071.19 -0.1022 0 IC40 1109.51 5.20953 1097.81 1118.78 0.0763 0.8 ICSO 1140.29 5.52815 1129.3 1150.27 0.1443 0.7 0 0.6 0.4 0.3 0.2-0.1 0.0 -0 500 1000 1500 Dose mgIL Dose-Response Plot 1-tail. 0.05 level of significance 25 2015.10 5, (f age 108 of 110 File: CdNaCICR_0508 12.xlsx Entered by: 1. Sur."e".Reviewed by:. 1TTA Page 5 o 6.Species: Ceriodaphnia dubia CdNaCICR #: _Daily Chemisty: I Day I Analyst I Concentration Par7ameter DO (mg/L)Ii-JI Conductivity (g~mhos/crn) CONTROL Alkalinity (me CaCO/L)Hardness (me CaCO-IL)--h Temperature (CC)m _EVED 0 -H7(S.U.)ff.14 Conductivity (gmhoq/CM) 600 mg NaCI/L Lea, I 'Ito Temperature (C)h i -VH (S.U.)DO (rot/L)800 mg NaCVL Conductivity (A mhs/cm)r IM F1 C-11+40-LS'M .Temperature (CC)I q -pH (S.U.)4PtII E -i 1 4% 1 DpO (rag/)Conductivity (gmhos/cm) 1000 mg NaCI/L L'f4 H *~s~~y I H 24,'.1 1.0 Temperature (°C)U U --r-uR (S.U.3~M F~ ~f-~7 L PH(- DO (mg/L)1200 mg NaCI/L ConductivityT (piuhos/cm) U. 0 ~ 'I 'Lq.9 IsoU 0 Temperature (°C)S~ ~ ~~t " S-. I WH (S.U.)I m 4.0l 1 1.7.I~~EA 1.~ I Conductivity I (Emh hs/cm)1400 mg NaCl/L?1.0 -7.I qw)I0-k UAL ZS. iZq.1?S. 0 I Temperature I(c)STOCK Conductivity I (p~nihos/cm) cco age 109 of 110 Initial SOP AT14 -Exhibit AT14.1, revision 06-01-11 ~71-Page 6 of 6 I Species: Ceriodaphnia dubia CdNaCICR #: I11 j 3-i TI LI I]II[.1-J*1 ii-I* 1~.1 I, Day___ __ __ __Analyst .Ak3 I Concentration Parameter HRS.U.) R~ Z o 60.o Conductivity '2-(gmhos/cm) CONTROL Alkalinity t.-p HardnessSI%(mg CaCO 3/L) Vi Temperature 5I".0 -" --".(0c) -- -LA -1 pH(S.U-) 7.3 f'4o q-.q6 %-6 0434 600 mg NaCI/L Conductivity I SIWO (Limhos/cm) Temperature V 8 114-0 1*.0cC)PH1 (S.U.) __-_1_ lis4.DO (mg/L) 4" +.800 mg NaCI/L Conductivity i 64o M l-47. 1120 Temperature "K -1.", PH (S.U.) 4'. 0 4. '4 0 DO (rg/L) -7,1 .4 ! ' ,"- ..1000 mg NaCI/L Conductivity --(g.mhos/cm)

Y)NO Temperature 1g I.' Fi4____ ____ ___ (0 C)DO (mgL) 1.- S 1200 mg NaCIIL Conductivity Tbl2 Temperature

_ .-" ..1 L0 pH_(S.U.) 1FI.s U 1400 mg NaCL/L Conductivity* tjo Temperature 7q 0w ~ so i'i L. ~ o W age 110 of 110 Initial II Fial I Initial F1 jinal j Initial II inali I Initial Fi iinal I-ii- h I -dl U mU SOP ATI4 -Exhibit ATI4.1, revision 06-01-11}}

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