ML13289A202
| ML13289A202 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 08/15/2012 |
| From: | Simmons P Tennessee Valley Authority |
| To: | Morgan C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| Shared Package | |
| ML13289A109 | List:
|
| References | |
| Download: ML13289A202 (164) | |
Text
{{#Wiki_filter:S58 120815 800- NPDES CORRESPONDENCE August15,2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR JULY 2012 Enclosed is the July 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. During July, natural conditions caused the 24-hour average ambient river temperature to exceed 29.4°C for 18 days, including 7/29 and 7/30 when the lnstream Monitoring temperature measured 30.6°C. The plant continued to operate in helper mode throughout this period and complied with the elevated 1-hour average river temperature limit of 33.9oc at the downstream edge of the mixing zone as required by Part 1.A.1.f of the NPDES permit. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. a:t Paul R. Simmons Plant Manager Signatory Authority for: John T. Carlin Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D. Booker, MOB 1F-WBN J.W. Proffitt, OPS 4C-SQN B. E. Brickhouse, BR 4A-C A. A. Ray, WT 11 A-K J. T. Carlin, OPS 4A-SQN G. R. Signer, WT 6A-K J. A. Cross, POB 2A-SQN P.R. Simmons, POB 2B-SQN T.R. Mgrkum, BR 4A-C B. N. Smith (EDMS), MPB 1E-M D. B. Nida, BR 4A-C
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 August15,2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR JULY 2012 Enclosed is the July 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. During July, natural conditions caused the 24-hour average ambient river temperature to exceed 29.4°C for 18 days, including 7/29 and 7/30 when the lnstream Monitoring temperature measured 30.6°C. The plant continued to operate in helper mode throughout this period and complied with the elevated 1-hour average river temperature limit of 33.9oC at the downstream edge of the mixing zone as required by Part 1.A.1.f of the NPDES permit. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Signatory Authority for: John T. Carlin Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUO~~UCLEARP~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Md~~~O.B~2000 ___________ _ ---~TEROFFICEOPS-5N-S~-------- TN0026450 101 G F- FINAL ---~DDY-~1~~~73~-------- PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Faqlli!y_ JYA- SEQUOYAH NUCLEAR PLANT_ _ ...;_ _ _ _ MONITORING PERIOD EFFLUENT ~ti~~IUONCOUN~---------- DAY I I YEAR I MO I DAY A TIN: Brad Love From 12 I 07 I 01 I To I 12 I 07 I 31
*** NO DISCHARGE D***
NOTE: Read instructions befo~ completin!i this fonn. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. SAMPLE EX TYPE WATERDEG. CONDUIT OR THRU ENT PLANT 1 0 GROSS NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my DATE direction or supervision in accordance with a system designed to assure that qualified personne Paul R. Simmons properly gather and evaluate the information submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, 1 - - - - - - - - - - - - - - - - - - l 423 843-6502 12 08 15 Sequoyah Plant Manager and complete. 1am aware that there are significant penalties for submitting false Information, SIGNATURE OF PRINCIPAL EXECUTIVE 1 - - - - - - - - - - - - - - - - - - l i n c l u d i n g the possibility of fine and Imprisonment for knowlng violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference afl attachments here} No closed mode operation. Veliger monitoring data is included as an attachment. Natural conditions caused the 24-hour average ambient river temperature to exceed 29.4"C for 18 days, including 7/29 and 7/30 when the lnstream Monitoring temperature measured 30.6"C. The plant continued to operate in helper mode throughout this period as required by Part 1.A.1.f of the NPDES permit. EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
Mean# of NOTES:% Mean# of Water Water Sample Date %Settlers Sample Date Asiatic LOCATION SUB LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp. fC) Temp.("C) Clams/m3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 PB 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 PB 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May 2012 No Samples Collected June 2012 No Samples Collected July 2012 No Samples Collected
PERMITTEE NAME/ADDRESS (Include Facility NameA..ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUO~~UCL~RP~NT _ _ _ _ DISCHARGE MONITORING REPORT {DMRJ (SUBR 01) OMB No. 2040-0004 AddresL_f.Q,_BOX2000 - - - - - - - - - - - - TN0026450 F- FINAL ---~TEROFFICEOP~5N-S~-------- ---~DDY-MI~JU73BL _______ _ PERMIT NUMBER BIOMONITORING FOR OUTFALL 101 Fa~~~A-SEQUO~HNUCL~RP~NC _ _ _ _ _ EFFLUENT l~i~~AMIUONCOUN~------~--- ATTN: Brad Love
*** NO DISCHARGE D ***
NOTE: Read instructions before completin~ this fonn. PARAMETER QUALITY OR CONCENTRATION NO. SAMPLE EX TYPE NAME/TITLE PRINCIPAl EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Paul R. Simmons properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, 1 - - - - - - - - - - - - - - - - - 1 423 843-6502 12 08 15 Sequoyah Plant Manager and complete. 1am aware that there are significant penalties for submiHing false information, SIGNATURE OF PRINCIPAL EXECUTIVE 1 - - - - - - - - - - - - - - - - - - ! i n c l u d i n g the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER Y~R MO DAY TYPED OR PRINTED CODE COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) Toxicity was not sampled in July 2012. EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location ;r Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM {NPDESJ MAJOR Form Approved. Name_~A-SEQUO~~UCL~RP~NT _ _ _ _ DISCHARGE MONITORING REPORT {DMRJ (SUBR 01) OMB No. 2040-0004 Address_...f..Q,_BOX2000 - - - - - - - - - - - - ---~TEROFFICEOP~N-S~-------- TN0026450 103 G F- FINAL ---~DDY-MISU~73~-------- PERMIT NUMBER LOW VOL. WASTE TREATMENT POND Fa~_~A-SEQUO~HNUCL~RP~NC ____ _ EFFLUENT L~U~~~I~NCOUN~---------- ATIN: Brad Love From Y~R 12 31
'*** NO DISCHARGE D '***
~-~--~----~
NOTE: Read instructions before completinn this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. SAMPLE EX TYPE 0 DUITORTHRU ITI:III:::AT*
- CI..IT P~NT
- A ~
6F-?fr NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my ..IELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Sequ,~ah Paul R. Simmons properly gather and evaluate the infonnation submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Plant Manager 423 843-6502 infonnalion, the infonnation submitted is , to lhe best of my knowledge and belief, true, aa:urate, 12 08 15 Sequoyah Plant Manager and complete. I am aware that there are significant penalties for submitting false infonnation, SIGNATURE OF PRINCIPAL EXECUTIVE 1 TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference aH attachments here) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUO~~UCL~RP~NT _ _ _ ~ DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 Addres.!__g.o. BOX_6900 - - - - - - - - - - - - TN0026450 F- FINAL ---~TEROFFICEOP~5N~~-------- ---~DDY-MIS~~738L _ _ _ _ _ _ _ _ RECYCLED COOLING WATER FaQ!i!y_ J)/A- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ EFFLUENT ~~ti~~IUONCOUN~---------- ATTN: Brad Love
*** NO DISCHARGE IXX I ***
NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. SAMPLE EX TYPE WATERDEG. ****--
-C, RATE OF NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER 1Certify under penalty of law that this document and all attachments were prepared under my DATE direction ar supervision in accordance with a system designed to assure that qualified personnel Paul R. Simmons properly gather and evaluate the information submiHed. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, 1---------~-------l 423 843-6502 12 08 15 Sequoyah Plant Manager and complete. I am aware that there are significant penalties for submiHing false information, SIGNATURE OF PRINCIPAL EXECUTIVE 1 - - - - - - - - - - - - - - - - - - - l i n c l u d i n g the possibility affine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. Name_~A-SEQUO~HNUCL~RP~NT _ _ ~- DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 Address _ _e.o. BOX20QQ.. _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICEOPS-5N~~-------- TN0026450 110 T F- FINAL ---~D~-MIS~~73BL _______ _ PERMIT NUMBER RECYCLED COOLING WATER Fa~-~A-SEQUO~HNUCl~RP~NL _ _ _ _ _ lo~ti~~~IUONCOUN~---------- EFFLUENT DAY ATTN: Brad Love 31
*** NO DISCHARGE IXX I ***
NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA
**-**- 23 TRP3B 0 0 'tfSEf.AI*** ' CQM!'QS EFFLUENT GROSS VALUE *>-*
IC25 STATRE 7DAY CHR SAMPLE M~SUREMENT PIMEPHALES TRP6C 1 0 0 'ol PERMIT' ,) '
- EFFLUENT GROSS VALUE SAMPLE M~SUR.EMENT
,,)r~u~.EBMrt:;>. F
, REQUIRE:ME:NT .
- , ';_: ~:'Q~=~~T~~~-::~~*::-~:~.~-,;~:},-::1 SAMPlE M~SUREMENT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TElEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Paul R. Simmons properly gather and evaluate the information submiHed. Based on my inquiry of lhe person or persons who manage lhe system, or those persons directly responsible for gathering lhe information, the Information submitted is. to the best o1 my knowledge and belief, true, accurate, 1 - - - - - - - - - - - - - - - - - - 1 423 843-6502 12 08 15 Sequoyah Plant Manager and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAl EXECUTIVE 1 - - - - - - - - - - - - - - - - - - l i n c l u d i n g the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER Y~R MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIO~TIONS (Reference all attachments here)
No Discharge this Period EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location ff Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~-~A-SEQUO~~UCL~RP~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMR} (SUBR 01) OMB No. 2040-0004 Addres.!_...f..fLBOX20~----------- ---~TEROFFICEOP&5N~~-------- TN0026450 F-FINAL ---~DDY-~1~~~738L _ _ _ _ _ ~-- PERMIT NUMBER WASTEWATER & STORM WATER Fa~-~A-SEQUO~HNUCL~RP~NC _ _ _ _ _ EFFLUENT w~ti~~IUONCOUN~---------- YEAR ATTN: Brad Love
- NO DISCHARGE I XX I ***
NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. SAMPLE EX TYPE AVERAGE MAXIMUM EFFLUENT GROSS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supeJVision in accordance with a system designed to assure that qualified personn Paul R. Simmons properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, t - - - - - - - - - - - - - - - - - - 1 423 843-6502 12 08 15 Sequoyah Plant Manager and complete. 1am aware that there are significant penalties for submiHing false information, SIGNATURE OF PRINCIPAL EXECUTIVE 1 - - - - - - - - - - - - - - - - - - - t i n c l u d i n g the possibility of fine ancl imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER Y~R MO DAY TYPED OR PRINTED CODE COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Prewous editions may be used Page 1 of 1
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- July 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 8/03/2012 CONCURRENCES Name R c Signature - Comment Date v N B.M. Love X L.M. Koby X J.A. Cross X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
S58 120910 800- NPDES CORRESPONDENCE September 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37219
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- DISCHARGE MONITORING REPORT (DMR) FOR AUGUST 2012 Enclosed is the August 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
- s\1,&~~
~11' " Site(lli~sident Seq't:teyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn : Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 S.D. Booker, MOB 1F-WBN J.W. Proffitt, OPS 4C-SQN B. E. Brickhouse, BR 4A-C A. A. Ray, WT 11 A-K J . T . Carlin , OPS 4A-SQN G. R. Signer, WT 6A-K J. A. Cross, POB 2A-SQN P.R. Simmons, POB 2B-SQN T.R. Markum , BR 4A-C B. N. Smith (EDMS), MPB 1E-M D. B. Nida, BR 4A-C
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 September 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville,Tennessee 37219
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- DISCHARGE MONITORING REPORT (DMR) FOR AUGUST 2012 Enclosed is the August 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. -.......""""'-'T=':*
~V e President Seq~ Nuclear Plant Enclosures cc (Enclosures):
Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013
REVIEW /CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- August 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 9/06/2012 CONCURRENCES Name R C Signature - Comment Date V N B.M. Love X L.M. Koby X J.A. Cross INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and
- indicate agreement with commitments made which are applicable to the revieWer's organization.
CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. Name_~A-SEQU~AANUCLEAAP~T _ _ _ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 ===(INTEROFFICEO~~N~QNL======= TN0026450 101 G F- FINAL ---~D~-MISY,T~7~L _______ _ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facility TVA- SEQUOYAH NUCLEAR PLANT L~tion HAMIUONCOUNrr========== MO 0
¥ o~9RING e;:wr MO DAY EFFLUENT D ...
A TIN: Brad Love 08 I 01 J Tor 12 I 08 31
*** NO DISCHARGE NOTE* Read instructions before completin~ this form X
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM ANALYSIS UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 31/31 MEASUREMENT ** 42.4 04 0 RCORDR CENTIGRADE 00010 1 0 PERMIT ******** ******** **** ******** ******** Req. Mon. DEG.C. CONTI CALCTD REQUIREMENT EFFLUENT GROSS DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** MEASUREMENT
******** ** 30.4 04 0 31/31 MODELD CENTIGRADE 00010 z 0 PERMIT ******** ******** **** ******** ******** 30.5 DEG.C. CONTI CALCTD REQUIREMENT INSTREAM MONITORING
------ DAILYMX -
NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ** ********- ******** 2 04 0 31/31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 s PERMIT ******** ******** **** ******** ******** 3 DEG.C. CONTI CALCTD REQUIREMENT EFFLUENT GROSS
* - - ---------- - - DAILYMX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** 1770 ******** ******** ******** 31/31 RCORDR MEASUREMENT 03 ** 0 TREATMENT PLANT 50050 1 0 PERMIT REQUIREMENT
******** Req. Mon. MGD ******** ******** ********
.... CONTI RCORDR EFFLUENT GROSS
- - - - - - - - - ---------** DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** 0.019 0.045 25/31 GRAB MEASUREMENT ** 19 0 50060 1 0 PERMIT ******** ******** **** ******** 0.1 0.1 MG/L FIVE PER CALCTD REQUIREMENT EFFLUENT GROSS
--- MOAVG DAILY MAX WEEK TEMPERATURE- C, RATE OF SAMPLE ******** 0 ******** ******** 31/31 CALCTD MEASUREMENT 62 ** 0 CHANGE 82234 1 0 PERMIT REQUIREMENT
******** 2 DEG ******** ******** ******* .... CONTI CALCTD C/HR EFFLUENT GROSS DAILYMX NUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT
%~
_ _ _ _ _ _ _ _ _ _ L.._ ____________ NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my a{fJM. TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel( John T. Carlin property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the L:f\ \.:7 _.. rgireJe'e President information, the information submitted is , to the best of my knowledge and belief, true, accurate, "" 423 843-7001 12 09 10 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, Si(;f'fA"'rURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility offine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: 1. Floguard MS6236 (max. calc. cone. was 0.032mg/L--Iimit 0.2mg/L) 2. Biodetergent 73551 (max. calc. cone. was 0.029mg/L--Iimit 2.0mg/L) 3. Spectrus CT1300 (max. calc. cone. was 0.037mg/L--Iimit 0.050mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
Mean# of NOTES:% Mean# of Water Water Sample Date %Settlers Sample Date Asiatic LOCATION SUB LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp.("C) Temp. ("C) Clams/m3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE 04/03/2012 229 0 18 04/03/2012 0 . 18 1-ISV-24-1234 PB 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 PB 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May 2012 No Samples Collected June 2012 No Samples Collected July 2012 No Samples Collected August 2012 No Samples Collected
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQU~AANUCLEARPUNT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres.!...__E.O. BOX2000 - - - - - - - - - - - - - _ _ .J!.NTEROFFICE OPS-5N-5QN)_ - - - - - - - TN0026450 101 T F- FINAL
~cilitv SODDY- DAISY. TN 37384
~-SEQU~HNUCL~RP~NT
~cati~~MILTONCOUN~----------
===== I PERMIT NUMBER MO f0NIT1RING DISCHARGE NUMBER PERI~t I BIOMONITORING FOR OUTFALL 101 EFFLUENT ATTN: Brad Love From Y~R 12 I 08 DAY 01 To [ 12 YEA MO 08 DAY I 31 *** NO DISCHARGE D ...
NOTE: Read instructions before completins:~ this form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE ?DAY CHR SAMPLE ******** ******** MEASUREMENT
******** ** Other ******** 23 CERIODAPHNIA TRP3B 1 0 PERMIT I ******** ******** **** 43.2 ******** ******** PERCENT SEMI COMPOS EFFLUENT GROSS REQ~~~EME~-T MINIMUM ANNUAL IC25 STATRE ?DAY CHR SAMPLE ******** Other ********
MEASUREMENT
******** ** ******** 23 PIMEPHALES TRP6C 1 0 PERMIT ******** ******** **** 43.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS
'-* ---** -----* MIMINUM ANNUAL SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
/ '/'l/7 /
k~~ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel
-----~ .* '
John T. Carlin Sequoyah Site Vice President properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information. the information submitted is . to the best of my knowledge and belief, true. accurate. and complete. I am aware that there are significant penalties for submitting false information,
'J 'fd bresident 423 843-7001 12 09 10
-~ATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. Ef-ICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was sampled August 12-17. A revised August DMR including toxicity results will be submitted during the next reporting period. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~_~A-SEQUO~~UCLEAAP~T _ _ _ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Addres.§_ __f_.O. BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ (INTEROFFICE OPS-SN-SQN) TN0026450 103 G F- FINAL ===SODDY-DAISY~~~4======== PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND ~ili~~A-SEQU~HNUCL~RPLAN~---- EFFLUENT Locati~~MILroNCOU~----------- ATTN: Brad love
*** NO DISCHARGE D ...
NOTE: Read Instructions before completln!l this form. PH 00400 1 PARAMETER 0
>< SAMPLE M~SUREMENT PERMIT AVERAGE QUANTITY OR LOADING MAXIMUM
******** l UNITS MINIMUM 7
6 QUALITY OR CONCENTRATION AVERAGE MAXIMUM 8 9 UNITS 12 su NO. EX 0 FREQUENCY SAMPLE OF ANALYSIS 14/31 THREE/ TYPE GRAB GRAB REQUIREMENT EFFLUENT GROSS
-***-- MINIMUM -*** MAXIMUM -
WEEK SOLIDS, TOTAL SUSPENDED SAMPLE
******** ******** ******** 7 9 0 2/31 GRAB M~SUREMENT ** 19 00530 1 0 PERMIT
******** ******** ** ******** 30 100 MG/L TWICE/ GRAB REQUIREMENT EFFLUENT GROSS
*-------- * - f-------*---* *- MOAVG DAILYMX MONTH f--.-~
OIL AND GREASE SAMPLE ******** ******** ******** <5 <6 0 2/31 GRAB M~SUREMENT ** 19 00556 1 0 PERMIT ******** ******** ** ******** 15 20 MG/l TWICE/ GRAB EFFLUENT GROSS FLOW, IN CONDUIT OR THRU REQUIREMENT SAMPLE M~SUREMENT 1.073 1.165 03 MOAVG DAILYMX 0 MONTH 31/31 RCORDR TREATMENT PLANT 50050 1 0 PERMIT REQUIREMENT Req. Mon. Req. Mon MGD ******** ******** ********
.. SEE RCORDR EFFLUENT GROSS MOAVG DAILYMX PERMIT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT 1-*
PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT I {c ~)$1 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the information, the information submitted is . to the best of my knowledge and belief, true. accurate. IT'7l . ~~p.. 'd t res1 en 423 843-7001 12 09 10 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIG~RE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. iER OR AUTHORIZED AGENT TYPED OR PRINTED OF AREA I NUMBER YEAR MO DAY CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. Na~-~A-SEQU~~NUCLEAAP~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 ===(INTEROFFICEO~~N~QNL======= TN0026450 110 G F- FINAL ---~DDY-MISY.T~738L _______ _ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facilitv TVA- SEQUOYAH NUCLEAR PLANT ~ation HAMI~NCOUNTI========== EFFLUENT DAY
*** NO DISCHARGE I XX I ***
ATTN: Brad Love 31 NOTE: Read instructions before completin!l this form X PARAMETER QUANTITI OR LOADING QUALITI OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TIPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** MEASUREMENT ** 04 CENTIGRADE 00010 1 0 PERMIT ******** ******** ** ******** ******** REPORT DEGC CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE
-- DAILYMX uous TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ********
MEASUREMENT ** 04 CENTIGRADE 00010 z 0 PERMIT ******** ******** ** ******** ******** 30.5 DEGC CONTIN CALCTD REQUIREMENT INSTREAM MONITORING s"AMPL_E___ -----* ------ - - - * - * * * *------- DAILYMX uous TEMP. DIFF. BETWEEN SAMP. & ******** ******** ******** ******** 04 MEASUREMENT ** UPSTRM DEG.C 00016 1 0 PERMIT ******** ******** ** ******** ******** 5 DEGC CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE
---------- f - - - - - - - * * ---- DAILYMX uous FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** ********
MEASUREMENT 03 ** TREATMENT PLANT 50050 1 0 PERMIT REQUIREMENT
******** Req. Mon. MGD ******** ******** ******** .. CONTIN RCORDR EFFLUENT GROSS VALUE
*--------- DAILYMX - - !-----***
uous
* - f---
SAMPLE ******** ******** CHLORINE, TOTAL RESIDUAL MEASUREMENT
******** ** 19 50060 1 0 PERMIT ******** ******** ** ******** 0.1 0.1 MG/L Five per CALCTD REQUIREMENT EFFLUENT GROSS VALUE MCH\VG DAILYMX Week TEMPERATURE- C, RATE OF SAMPLE ******** 04 ******** ******** ******** **
MEASUREMENT CHANGE 82234 1 0 PERMIT REQUIREMENT
******** 2 DEGC f---
.. CONTIN CALCTO EFFLUENT GROSS VALUE DAILYMX uous SAMPLE MEASUREMENT PERMIT REQUIREMENT I
-** - --~
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all atlachments were prepared under my TELEPHONE DATE direction or supervision in accordance wllh a system designed to assure that qualified personne John T. Carlin properly gather and evaluate the information submitled. Based on my inquiry of lhe person or persons who manage the system, or those persons directly responsible for gathering lhe ICe President informalion, the information submitled is , to lhe best of my knowledge and belief, true, accurate, f-\:-=:-:n&.':-::'::::-:=-::-:=::::::=-:c:-:===::-:=---1 423 843-7001 12 09 10 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitling false information, J-----=-:=::::-:::-::--::c::-:::-::==--------jincluding the possibility of fine and imprisonment for knowing violations. AREA NUMBER TIPED OR PRINTED YEAR MO DAY CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQU~~NUCLEAAP~NT ___ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 ---(iNTEROFFICE OPS-5N.SQN)- - - - - - - - TN0026450 110 T F- FINAL ---wDm-~lsY.T~73s4 _ _ _ _ _ _ _ _ ~cilitv ~-SEQU~HNUCL~RP~NT ~cati~~MI~NCOUN~----------
===== PERMIT NUMBER fO~RING DISCHARGE NUMBER P~Ot MO RECYCLED COOLING WATER EFFLUENT ATTN: Brad Love From I YEAR 12 MO I 08 oL DR To 112 08 I 3~
DAY I *** NO DISCHARGE I XX I *** NOTE: Read instructions before completinQ this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ******** M~SUREMENT ** 23 CERIODAPHNIA f--* TRP3B 1 0 0 PERMIT ******** ******** **** 43.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE
**---***-* *--------* MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ********
M~SUREMENT ** 23 PIMEPHALES TRP6C 1 0 0 PERMIT ******** ******** **** 43.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE
------- ----- MINIMUM ANNUAL SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel / *'L~ TELEPHONE DATE John T. Carlin Sequoyah Site Vice President properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the a ~ Si~: 1 President information, the information submitted is
- to the best of my knowledge and belief, true, accurate, 1---::::-:::-:-+/-~~==-==:-:-:::=:c:-:=====-=---i 423 843-7001 12 09 10 and complete. I am aware that there are significant penalties for submitting false information. SIGNA OF PRINCIPAL EXECUTIVE 1 - - - - - - - , - - - - - - - - - - - - - - - i i n c l u d i n g the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA TYPED OR PRINTED NUMBER Y~R MO DAY CODE COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR TVA- SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMS No. 2040-0004 (SUBR 01) TN0026450 118 G F- FINAL PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER to~T~RING Ip~WDI I EFFLUENT I YEAR I MOD y MO DAY IXX I *** Tor 12 I 08 I 31
*** NO DISCHARGE ATTN: Brad Love From 12 08 01 NOTE: Read instructions before completin!l this form.
X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT
- ******** ******** 19 00300 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
******** ******** -- MINIMUM 2 ******** ******** MG/L TWICE/
WEEK GRAB SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT
- ******** ******** 19 00530 1 0 PERMIT REQUIREMENT
******** ******** -- ******** ******** 100 DAILYMX MG/L TWICE/
WEEK GRAB EFFLUENT GROSS
*--------- ~---
SOLIDS, SETTLEABLE SAMPLE ******** ******** ******** ******** 25 MEASUREMENT 00545 1 0 PERMIT REQUIREMENT
******** ******** -- ******** ******** 1 DAILYMX ML/L ONCE/
MONTH GRAB EFFLUENT GROSS SAMPLE FLOW, IN CONDUIT OR THRU 03 ******** ******** ******** TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ******** ******** ********
- ONCE/ ESTIMA REQUIREMENT EFFLUENT GROSS _MOAVG __ DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
~/1 I
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel
~'
John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information. the information submitted is
- to the best of my knowledge and belief. true, accurate, 7 .,_. 1 e resident 423 843-7001 12 09 10 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false Information. SIGNAUJP(E OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
S58 12101 0 800- NPDES CORRESPONDENCE October 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37219
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- DISCHARGE MONITORING REPORT (DMR) FOR SEPTEMBER 2012 AND REVISED DMR FOR AUGUST 2012 Enclosed is the September 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The toxicity report and revision to Discharge Number 101T, Biomonitoring for Outfall101 for August 2012, are also attached. There were no exceedances during the monitoring period. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or* by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D. Booker, MOB 1F-WBN J.W. Proffitt, OPS 4C-SQN B. E. Brickhouse, BR 4A-C A. A. Ray, WT 11A-K J. T. Carlin, OPS 4A-SQN G. R. Signer, WT 6A-K J. A. Cross, POB 2A-SQN P.R. Simmons, POB 2B-SQN T.R. Markum, BR 4A-C B. N. Smith (EDMS), MPB 1E-M D. B. Nida, BR 4A-C
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 October 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville,Tennessee 37219
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- DISCHARGE MONITORING REPORT (DMR) FOR SEPTEMBER 2012 AND REVISED DMR FOR AUGUST 2012 Enclosed is the September 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The toxicity report and revision to Discharge Number 101T, Biomonitoring for Outfall101 for August 2012, are also attached. There were no exceedances during the monitoring period. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at {423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
\
Site ic resident Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAl POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} MAJOR Form Approved. N~~-~A-SEQUO~HNUCL~RPUNT~--- DISCHARGE MONITORING REPORT (DMRJ (SUBR01) OMB No. 2040-0004 Addres.l!__f.O. BOX2000 - - - - - - - - - - - - - - ---~TEROFFICEOPS-5N-S~-------- TN0026450 101 G F- FINAL
- - - _ ~ODDY- DAISYJN~7384_ - - - - - - - - PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Fa~-~A-S~~HNUCL~RP~NC _____
IY~RI ~j :~:i:. To\12 I ~~-~ ~~: t~N~~RING P~D EFFLUENT
~~~~MI~NCOUN~----------
ATTN: Brad Love From: 12 : I
- NO DISCHARGE o-NOTE* Read ilistructlons before completing this fonn PARAMETER TEMPERATURE, WATER DEG.
>< SAMPLE M~SUREMENT AVERAGE QUANTITY OR LOADING MAXIMUM UNITS MINIMUM QUALITY OR CONCENTRATION AVERAGE MAXIMUM 41.3 UNITS 04 NO. FREQUENCY SAMPLE EX 0
OF ANALYSIS 30/30 TYPE RCORDR CENTIGRADE 00010 1 0 PERMIT REQUIREMENT
........... '. Req.Mon* DEG.C. CONTI CALCTD EFFLUENT GROSS DAILY MAX NUOUS SAMPLE ******** ******** ********
TEMPERATURE, WATER DEG. M~SUREMENT
******** ** 29.1 04 0 30/30 MODELD CENTIGRADE 00010 z 0 PERMIT 30.5 DEG.C. CONTI CALCTD REQUIREMENT INSTREAM MONITORING DAILYMX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** 3 30/30 CALCTD M~SUREMENT ** o4 0 UPSTRM DEG.C 00016 1 s PERMIT ***-* 3 DEG.C. CONTI CALCTO REQUIREMENT EFFLUENT GROSS DAILYMX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** 1761 ******** ******** ********" 30/30 RCORDR 0
TR~TMENT PLANT 50050 1 EFFLUENT GROSS 0 M~SUREMENT PERMIT REQUIREMENT
**-** Req. Mon.
DAILY MAX 03 MGD ........ ........ ........ **
.... CONTI NUOUS RCORDR CHLORINE, TOTAL RESIDUAL SAMPLE M~SUREMENT
- ........ ******** 0.017 0.032 19 0 24/30 GRAB 50060 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
*-*** *- MOAVG 0.1 0.1
*DAILYMAX MGIL FIVE PER CALCTD WEEK TEMPERATURE- C, RATE OF SAMPLE ******** 0 ******** ******** 0 30/30 CALCTD M~SUREMENT 62 **
CHANGE 82234 1 0 PERMIT REQUIREMENT 2 DEG -**** .... .CONTI CALCTD EFFLUENT GROSS C/HR DAILYMX NUOUS SAMPLE M~SUREMENT PERMIT REQUIREMENT
- NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my diredion or supervision in accordance with a &Ystem designed* to assure that qualified personnel v (\~
' ~
n 1} TELEPHONE DATE John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or ( /."1 f A_ ; . persons who manage the system, or those persons directly responsible for gathering the < * ::..1 ~ ~eVice President Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 1-~
"d+/--:+/-::-::=~-=="'==:-:-:=:=c:::::::-=--i 423 843-7001 . 12 10 10 and complete. I am aware that there are significant penalties for submiHing false information, S~'rllRE OF PRINCIPAL EXECUTIVE I
(-----====--==c====-------!including the possibility of fine and Imprisonment tor knowing violations. TYPED OR PRINTED OFFicER OR AUTHORIZED AGENT AREA CODE I NUMBER ~R MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments !Jere) No closed mode operation. Veliger monitoring data is included as ari attachment.' The following injections occurred: 1. Flogoard MS6236 (max. calc. cone. was 0.057mgll-limit 0.2mgll) 2. Biodetergent 73551 (max. calc. cone. was 0.038mg/L-Iimit 2.0mg/L) 3. Spectrus CT1300 (max. calc. cone. was 0.037mgll-limit 0.050mg/L) EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
Mean# of NOTES;% Mean# of Water
- Water Sample Date %Settlers Sample Date Asiatic:
- LOCATION SUB LOCATION Gravid Asiatic: COLLECTED BY ZM/m3 Temp.("C) Temp.("C)
Clams/m3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB 01/24/2012 0 0 13 01/24/2012 0 .13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 PB 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234. PB 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May2012 No Samples Collected June 2012 No Samples Collected July2012 No Samples Collected August2012 No Samples Collected September 2012 No Samples Collected
PERMmEE NAME/ADDRESS (Include FacilitY Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Foim Approved.
- Name Address P.O.BOX2000 ============
TVA- SEQUOYAH NUCLEAR PLANT ---~TE~FFICEOPS-5N-S~-------- --~~D~-~ISU~73~--------
. DISCHARGE MONITORING REPORT (DMRJ (SUBR F-FINAL 01)
BIOMONITORING FOR OUTFALL OMB No. 2040-0004 101 ~~-~A-SEQUO~HNUCL~R~N~---- EFFLUENT ~~~~AMI@NCOUN~---------- ATTN: Brad Love
*** NO DISCHARGE D ***
NOTE: Read instructions before completing this form. L>< PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE
******** ******** ** Monitoring ******** ******** 23 M~SUREMENT CERIODAPHNIA Not Req_uired TRP3B 1 0 PERMIT ******** 43~2 ******** *****- PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE
******** ******** ** Monitoring ******** ******** 23 M~SUREMENT PIMEPHALES Not Required TRP6C 1 0 PERMIT ******** **-** '43.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MIMINUM ANNUAL SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT
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((X l '/ JJJ . NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of Jaw that this document end all aHachments were prepared under my . TELEPHONE DATE direction or supervision In accoidance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the info!lllation submitted. Based on my Inquiry of the. person or persons who manage the system, or those persons dlreclly responsible for gathering the information, the information submilled is , to the beat of my knowledge and belief, true, accurate, - vtce President 423 843-7001 12 10 10 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIG_~r PRINCIPAL EXECUTIVE l TYPED OR PRINTED including tha*possibility of fine and imprisonment for knowing violations. OFFIC OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in September 2012. EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. Na~-~A-SEQUO~HNUCL~RPUNT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ (SUBR01) OMB No. 2040-0004 AddreS!..._ ...f.Q...BOX 2000 _ _ - - - - - - - - - - ---~TE~FFICEOPS~N-S~--------
- TN0026450 . 103 G F- FINAL
---~D~-MI~~~73~-------- LOW VOL. WASTE TREATMENT POND Fa~-~A-SEQU~H~L~RP~NC _ _ ~-- EFFLUENT ~ti~~~I~NCOUN~---------- ATTN: Brad Love
- NO DISCHARGE D -
NOTE* Read instructions before completing this form C>< PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMF'LE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS SAMPLE ******** 7 ******** PH M~SUREMENT
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*/ * \r)r, n NAME/TITLE PRINCIPAL EXECUTNE OFFICER 1/1\ I;:;_ ~
I Certify under penalty of Jaw that this document and all attachments were prepared under my I TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel
'*-~w ~~President John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 12 10 10 and complete. I am aware that there are significant penalties for submitting false information, Sl~ PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations.
IC OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPlANATION OF ANY VIO~TIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Fonn Approved. Na~-~A-SEQUO~~UCL~RPUNT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 _ _E.Q...BOX2000 - - - - - - - - - - - - Address ---~E~FFICE0~-5N-S~-------- TN0026450 110 G F- FINAL -~-~~~-M~~~73~-------- PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER ~~-~A-SroU~HNUCL~R~NC _ _ _ _ _ L~~~AMI~NCOUN~---------- I Y~R MO M%~JORING PERI~D YEA MO {)AY
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EFFLUENT rxx I ATTN: Brad Love Fro.m 12 I 09 I 01 I To !12 I 09 I 30 *** NO DISCHARGE *** NOTE* Read instructions before completinR this fonn X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ****.'**** ******** CENTIGRADE 00010 1 0 EFFLUENT GROSS VALUE M~SUREMENT PERMIT REQUIREMENT.
** ******** ........ REPORT DAILYMX 04 DEGC CONTIN uous CALCTD TEMPERATURE, WATER DEG. . SAMPLE ******** ******** '******** ********
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NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law tl)at this documarif and all attachments wera praperad under my TELEPHONE John T. Carlin Sequoyah Site Vice President direction or supervision In ai:cordance with a system dasigned to assure that qualified personna~ properly gathar and avaluata tha information submitted. Based on my inquiry of tha person or persons who managa tha system, or those persons directly responsible for gathering the . information*.the information su~ittad is , to tha bast of my knowledge and belief, true, SC!'Urata. let~ ~ .. 423 843-7001 12 DATE 10 10 and complate. I am awera that thara ara significant penalties for submitting falsa information, Sli!NI~_E OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED Including tha possibility of !ina and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here} No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMmEE NAME/ADDRESS (Include Facility NameJLoca(;on if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDE.S} MAJOR Fonn Approved. Name Address P.O. BOX2000 ============ TVA- SEQUOYAH NUCLEAR PLANT ---~TE~FFICEOP~N-S~-------- ---~D~-M1~~~73~-------- DISCHARGE MONITORING REPORT (DMRJ (SUBR01) F-FINAL RECYCLED COOLING WATER OMB No. 2040-0004 ~~-~A-S~U~HNUCL~P~NC---~- EFFLUENT L~~~~I@NCOUN~----------
*** NO DISCHARGE IXX I ***
A TIN: Brad Love NOTE: Read instructions before completing this fonn X PARAMETER QUANnTY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS IC25 STATRE 7DAY CHR SAMPLE MEASUREMENT AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 23 CERIODAPHNIA TRP3B 1 0 0 PERMIT **'"'**** **'"'**" 43.2 ***"*** *"**- PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE MEASUREMENT
- ******** ******** 23 PIMEPHALES TRP6C 1 p 0 PERMIT ******** 43.2 **"'"'** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT*
REQUIREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OF'FICER I Certify under penally of law that this document end all attachments -re prepared under my John T. Carlin direction or supervision In accordance with a systam designed to assure that qualified personnel properly gather and evaluata the information submitted. Based on my inquiry of the person or persons who manage the system, or thosa persons directly responsibla for gathering tha (Arw
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President TELEPHONE DATE information, the information submitted is , to the best of my knowiedga and belief, ln!e. accurate, 423 843-7001 12 10 10 Sequoyah Site Vice President and complete. I ain aware that there are significant penalties for submitting false information, SIGNA;r'URE OF PRINCIPAL EXECUTIVE I lYPED OR PRINTED including the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) PreVious editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility NameA.ocatlon if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~-~A-S~UO~HNUCL~RP~NT _ _ _ _ DISCHARGE MONITORING REPORT . (DMR) (SUBR01) OMB No. 2040-0004 Addres!._ _e.Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ ._ _ ..:...._ _ _ .J!.NTEROFFICE OPS-5N-SQID-'- _ _ _ _ _ _ TN0026450 118 G F- FINAL ~--~D~-MISQ~73~-------- PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER
~~-~A-S~U~HNUCL~RP~NC _ _ _ _ _
L~~~~NCOUN~---------- I . t~RING P~D YEAR MO MO DAY I EFFLUENT IXX I *** From 12 I 09 o1 To 112 I 09 I 30 *** NO DISCHARGE ATTN: Brad Love NOTE: Read Instructions tiefore completing this form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO) SAMPLE ******** ******** ********
** ******** 19 00300 1 0 MEASUREMENT PERMIT REQUIREMENT
........ *-**** - 2 ........ ......... MGIL TWICE/
WEEK GRAB EFFLUENT GROSS MINIMUM SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** ******** M~SUREMENT ** 19 00530 EFFLUENT GROSS 1 0 PERMIT. REQUIREMENT *-**** ******** - ******** ******** 100 DAILYMX MGIL TWICE/ WEEK GRAB SOLIDS, SETTL~BLE SAMPLE ******** ******** ******** ******** MEASUREMENT ** 25 00545 1 0 PERMIT REQUIREMENT -*-** *-**** - "****** "****** 1 MUL ONCE/ GRAB EFFLUENT GROSS DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** MEASUREMENT 03 ** TR~TMENT PLANT 50050 1 0 PERMIT Req. Mon. Req.Mon. MGD ******** ******** . ********
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under JlEII!ally of law that this doaJment and all aHachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system deaigned to easure that qualified personnal John T. Carlin properly gather and evaluate the information submitted. Baaed on my inquiry of the person or persons who manage the system, or thOSe persons directly reaponslbla for gathering tha information, the information submitted is , to the beat of my knowiedge and belief, true, accurate, 423 843-7001 12 10 10 Sequoyah Site Vice President and complate. I am aware that there are significant penaltiea for submitting false information, SIGNA'tORE OF PRINCIPAL EXECUTNE I TYPED OR PRINTED lnduding the possibility of fine and imprisonmant for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REv 3199) Previous editions may be used Page 1 of 1
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) (SUBR01) OMB No. 2040-0004 F-FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT ATTN: Brad Love
*** NO DISCHARGE D ***
NOTE* Read instnictions before complelina this form X PARAMETER QUANnTY OR LOADING QUALITY OR CONCENTRAnON NO. FREQUENCY SAMPLE EX *oF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 EFFLUENT GROSS 0 SAMPLE MEASUREMENT PERMIT REQUIREMENT
** >100.0 43.2 MINIMUM 23 PERCENT 0 1/180 SEMI ANNUAL COMPOS COMPOS JC25 STATRE 7DAY CHR SAMPLE ******** ******** >100.0 ******** 1/180 COMPOS MEASUREMENT ** ******** 23 0 PIMEPHALES ..
TRP6C 1 0 PERMIT ******** ******** ...... 43.2 ******** ******** PERCENT .SEMI COMPOS REQUIREMENT EFFLUENT GROSS MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
~PLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT
*REQUIREMENT NAME/TITLE PRINCIPAL EXECUnVE: OFFICER I Certify under penalty of lew that this document and all eHachments were prepared un.der my TELEPHONE DATE direclion or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and 'evaluate the information submitted. Based on my inquiry of the.person or parsons who manage the system, or those parsilns directly responsible for gathering the '--Js:Milid,(t~~fiice President Sequoyah Site Vice President information, the information submitted Is. to the best of my knowledge and belief, true, accurate, l--~~l-:-:-+/-:-::-:::-:::-=::-:-::-:-:::-:-:--=c=-:,.---::-:::=---1 423 843-7001 12 10 10 and complete. I am aware that there are significant penalties tor submitting false information, SIG E OF PRINCIPAL. EXECUTIVE 1 - - - - - - - - - - - - - - - - - - J i n c l u d i n g the possibility of line and imprisonment for knowing violations. . OFFICER OR AUTHORIZED AGENT NUMBER TYPED OR PRINTED AREA YEAR MO DAY CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity sampling began on August 12 and ended on August 17. This is the revised August 2012 DMR data set for 101T. The toxicity report is attached. EPA Fonn 3320-1 (REV 3199) PreVious editions may be used Page 1 of 1
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: September 17, 2012
- 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 1,579
- 6. Receiving Stream: Tennessee River (TRM 483.6)
- 7. 1Q10: 3,491
- 8. Outfall Tested: 101
- 9. Dates Sampled: August 12 - 17, 2012
- 10. Average Flow on Days Sampled (MGD): 1741.838, 1748.488, 1747.571
- 11. Pertinent Site Conditions: Production / operation data will be provided upon request.
- 12. Test Dates: August 14 - 21, 2012
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia)
- 15. Concentrations Tested (%): Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100 Pimephales promelas: UV treated Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100
- 16. Permit Limit Endpoint (%): Outfall 101: IC25 = 43.2%
- 17. Test Results: Outfall 101: Pimephales promelas: IC25 > 100%
Ceriodaphnia dubia: IC25 > 100% UV treated Outfall 101: Pimephales promelas: IC25 > 100% Page 1 of 99
- 18. Facility
Contact:
Brad Love Phone #: (423) 843-6714
- 19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Donald W. Snodgrass Phone #: (256) 386-2787
- 22. Notes: Outfall 101 samples collected August 12 - 17, 2012, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were
> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 2 of 99
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101, Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (°C) (mg/L) Last Used By 08-12-12 0645 to 08-14-12 1213 101 08-13-12 1540 0.8, 0.7 <0.10 08-13-12 0545 08-15-12 1115 08-12-12 0705 to 08-14-12 1213 Intake 08-13-12 1540 0.9 <0.10 08-13-12 0605 08-15-12 1115 08-14-12 0700 to 08-16-12 1114 101 08-15-12 1553 1.9, 1.9 <0.10 08-15-12 0600 08-17-12 1115 08-14-12 0720 to 08-16-12 1114 Intake 08-15-12 1553 2.0 <0.10 08-15-12 0620 08-17-12 1115 08-18-12 1127 08-16-12 0700 to 101 08-17-12 1338 2.2, 2.2 <0.10 08-19-12 1115 08-17-12 0600 08-20-12 1114 08-18-12 1127 08-16-12 0715 to Intake 08-17-12 1338 2.4 <0.10 08-19-12 1115 08-17-12 0615 08-20-12 1114
*TRC = Total Residual Chlorine Samples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0oC) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Page 3 of 99
Pimephales promelas Ceriodaphnia dubia Test Organisms:
- 1. Source: Aquatox, Inc. In-house Cultures
- 2. Age: 20 - 20.22 hours old < 24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate: 10 1
- 6. Test Initiation: (Date/Time)
Outfall 101 08-14-12 1200 ET 08-14-12 1148 ET UV Treated Outfall 101 08-14-12 1213 ET
- 7. Test Termination: (Date/Time)
Outfall 101 08-21-12 1106 ET 08-21-12 1053 ET UV Treated Outfall 101 08-21-12 1122 ET
- 8. Test Temperature: Outfall 101: Mean = 24.7oC Mean = 24.9oC (24.3 - 25.1oC) (24.6 - 25.3oC)
Test Temperature: UV-Treated Outfall 101: Mean = 24.8oC (24.2 - 25.1oC)
- 9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
Page 4 of 99
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration) Conducted August 14 - 21, 2012 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 95 Mean Dry Weight (mg) Test Solutions (replicate number) (% Effluent) 1 2 3 4 Mean Control 0.583 0.615 0.592 0.544 0.584 10.8% 0.535 0.669 0.686 0.649 0.635 21.6% 0.608 0.559 0.626 0.686 0.620 43.2% 0.582 0.563 0.487 0.614 0.562 86.4% 0.614 0.555 0.563 0.690 0.606 100.0% 0.532 0.673 0.506 0.571 0.571 Intake 0.537 0.569 0.551 0.687 0.586 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.2% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LC50: TUc = 100/ IC25 Page 5 of 99
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration) Conducted August 14 - 21, 2012 using effluent from Outfall 101. Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 33 29 33 31 28 34 32 30 32 31.2 10.8% 36 34 33 33 35 35 34 34 31 32 33.7 21.6% 36 31 33 31 30 37 35 33 32 30 32.8 43.2% 37 34 34 31 31 31 37 34 34 35 33.8 86.4% 34 34 36 34 35 33 34 35 36 37 34.8 100.0% 38 37 37 34 32 38 35 39 37 38 36.5 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.2% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LC50: TUc = 100/ IC25 Page 6 of 99
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration) Conducted August 14 - 21, 2012 using water from Intake Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 34 34 31 32 29 32 31 29 31 31.3 Intake 33 29 33 33 33 36 33 32 36 34 33.2 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LC50: TUc = 100/ IC25 Page 7 of 99
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration) Conducted August 14 - 21, 2012 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 98 98 98 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg) Test Solutions (replicate number) (% Effluent) 1 2 3 4 Mean Control 0.510 0.488 0.587 0.553 0.535 10.8% 0.673 0.559 0.631 0.594 0.614 21.6% 0.599 0.524 0.547 0.582 0.563 43.2% 0.592 0.516 0.548 0.588 0.561 86.4% 0.509 0.527 0.551 0.448 0.509 100.0% 0.550 0.565 0.526 0.494 0.534 Intake 0.621 0.573 0.647 0.482 0.581 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LC50: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration Toxicant Results (IC25) Pimephales promelas August 14 - 21, 2012 1225 7 days KCl 0.81 g/L Ceriodaphnia dubia August 07 - 14, 2012 1045 7 days NaCl 1.08 g/L Page 8 of 99
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephales promelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed August 14-21, 2012. Test Sample ID o Page 9 of 99 Temperature ( C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (µmhos/cm) (mg/L CaCO3) (mg/L CaCO3 ) Chlorine (mg/L) 24.8 24.7 7.7 7.9 7.55 7.50 311 62 89 Control 24.7 - 24.9 24.6 - 25.1 7.6 - 7.9 7.8 - 8.0 7.43 - 7.67 7.36 - 7.59 302 - 323 62 - 62 88 - 92 24.8 24.6 7.9 7.8 7.52 7.46 291 10.8% 24.7 - 25.0 24.3 - 25.0 7.7 - 8.0 7.6 - 8.0 7.44 - 7.62 7.33 - 7.55 285 - 298 24.8 24.6 7.9 7.8 7.54 7.44 278 21.6% 24.7 - 25.0 24.4 - 24.9 7.7 - 8.0 7.6 - 8.0 7.44 - 7.62 7.33 - 7.55 272 - 283 24.8 24.6 7.9 7.8 7.53 7.44 252 43.2% 24.7 - 25.0 24.4 - 24.8 7.7 - 8.0 7.6 - 8.0 7.45 - 7.61 7.34 - 7.52 246 - 259 24.8 24.6 7.9 7.8 7.51 7.42 198 86.4% 24.7 - 25.0 24.3 - 24.8 7.6 - 8.0 7.6 - 7.9 7.45 - 7.60 7.31 - 7.52 192 - 205 Pimephales promelas 24.9 24.6 7.9 7.9 7.51 7.46 182 71 72 < 0.10 100.0% 24.8 - 25.0 24.4 - 24.8 7.7 - 8.1 7.7 - 8.1 7.42 - 7.60 7.32 - 7.57 177 - 186 70 - 72 71 - 74 < 0.10 - < 0.10 24.9 24.6 8.0 8.0 7.51 7.45 182 69 73 < 0.10 Intake 24.7 - 25.0 24.4 - 24.7 7.7 - 8.2 7.9 - 8.0 7.43 - 7.61 7.33 - 7.54 176 - 186 68 - 70 72 - 74 < 0.10 - < 0.10 24.8 25.1 7.7 8.0 7.55 7.51 311 62 89 Control 24.7 - 24.9 25.0 - 25.2 7.6 - 7.9 7.8 - 8.2 7.43 - 7.67 7.28 - 7.59 302 - 323 62 - 62 88 - 92 24.8 25.0 7.9 8.0 7.52 7.51 291 10.8% 24.6 - 25.0 24.8 - 25.2 7.7 - 8.0 7.8 - 8.2 7.44 - 7.62 7.29 - 7.59 285 - 298 24.8 25.0 7.9 8.0 7.54 7.52 278 21.6% 24.7 - 25.0 24.8 - 25.1 7.7 - 8.0 7.8 - 8.2 7.44 - 7.62 7.30 - 7.60 272 - 283 24.9 24.9 7.9 8.0 7.53 7.52 252 43.2% 24.7 - 25.0 24.8 - 25.3 7.7 - 8.0 7.9 - 8.2 7.45 - 7.61 7.30 - 7.61 246 - 259 24.9 25.0 7.9 8.0 7.51 7.51 198 86.4% 24.7 - 25.1 24.8 - 25.2 7.6 - 8.0 7.9 - 8.2 7.45 - 7.60 7.28 - 7.61 192 - 205 Ceriodaphnia dubia 25.0 24.9 7.9 8.0 7.51 7.52 182 71 72 < 0.10 100.0% 24.8 - 25.1 24.8 - 25.2 7.7 - 8.1 7.9 - 8.3 7.42 - 7.60 7.29 - 7.63 177 - 186 70 - 72 71 - 74 < 0.10 - < 0.10 25.0 24.9 8.0 8.1 7.51 7.52 182 69 73 < 0.10 Intake 24.8 - 25.1 24.7 - 25.1 7.7 - 8.2 8.0 - 8.2 7.43 - 7.61 7.30 - 7.64 176 - 186 68 - 70 72 - 74 < 0.10 - < 0.10 o Overall temperature ( C) Average Minimum Maximum Pimephales promelas 24.7 24.3 25.1 Ceriodaphnia dubia 24.9 24.6 25.3
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephales promelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed August 14-21, 2012. Test Sample ID o Temperature ( C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness *Total Residual Initial Final Initial Final Initial Final (µmhos/cm) (mg/L CaCO3 ) (mg/L CaCO3 ) Chlorine (mg/L) Page 10 of 99 24.8 24.7 7.9 7.8 7.59 7.49 304 61 84 Control 24.8 - 24.9 24.5 - 24.9 7.8 - 8.1 7.6 - 7.9 7.50 - 7.65 7.34 - 7.61 298 - 316 61 - 62 84 - 84 24.9 24.7 7.9 7.8 7.60 7.48 291 10.8% 24.8 - 25.0 24.5 - 25.0 7.9 - 8.0 7.6 - 7.8 7.50 - 7.66 7.34 - 7.57 282 - 298 24.9 24.7 7.9 7.8 7.59 7.48 279 21.6% 24.8 - 25.1 24.6 - 24.8 7.9 - 8.0 7.7 - 7.9 7.50 - 7.66 7.34 - 7.58 271 - 284 24.9 24.7 7.9 7.8 7.58 7.47 254 43.2% 24.8 - 25.1 24.6 - 24.8 7.9 - 8.0 7.7 - 7.9 7.49 - 7.66 7.33 - 7.58 246 - 258 25.0 24.7 8.0 7.8 7.56 7.46 203 86.4% 24.9 - 25.1 24.3 - 24.8 7.9 - 8.1 7.5 - 7.9 7.48 - 7.63 7.33 - 7.57 197 - 207 Pimephales promelas 25.1 24.6 8.0 7.8 7.55 7.45 184 70 72 < 0.10 100.0% 25.0 - 25.1 24.2 - 24.7 7.8 - 8.3 7.5 - 7.9 7.47 - 7.62 7.31 - 7.55 178 - 188 70 - 70 72 - 72 < 0.10 - < 0.10 25.0 24.6 8.1 7.9 7.54 7.46 184 70 72 < 0.10 Intake 24.8 - 25.1 24.5 - 24.9 7.9 - 8.2 7.7 - 8.0 7.46 - 7.61 7.32 - 7.58 178 - 191 70 - 70 71 - 72 < 0.10 - < 0.10
*Note: Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.
o Overall temperature ( C) Average Minimum Maximum Pimephales promelas 24.8 24.2 25.1
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected August 12 - 17, 2012, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 11 of 99
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None Page 12 of 99
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B.
- 4. Dissolved oxygen was measured by SM 4500 O G.
- 5. The pH was measured by SM 4500 H+ B.
- 6. Conductance was measured by SM 2510 B.
- 7. Alkalinity was measured by SM 2320 B.
- 8. Total hardness was measured by SM 2340 C.
- 9. Total residual chlorine was measured by ORION Electrode Method 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCl.
- 2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 13 of 99
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Standard Methods for the Examination of Water and Wastewater, 21st Edition, 2005.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of 99
Sequoyah Nuclear Plant Biomonitoring August 14 - 21, 2012 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 03/12/1998 0.016 - - - - - - 03/13/1998 0.015 - - - - - - 03/14/1998 0.013 - - - - - - 03/15/1998 0.030 - - - - - - 03/16/1998 0.013 - - - - - - 03/17/1998 0.020 - - - - - - 03/18/1998 0.018 - - - - - - 09/08/1998 0.015 - 0.014 0.005 - - 0.021 09/09/1998 0.003 - 0.031 0.011 - - - 09/10/1998 0.014 - 0.060 0.021 - - - 09/11/1998 0.013 - 0.055 0.019 - - - 09/12/1998 < 0.001 - 0.044 0.015 - - - 09/13/1998 < 0.001 - 0.044 0.015 - - - 09/14/1998 0.008 - 0.044 0.015 - - - 02/22/1999 < 0.001 - - - - - - 02/23/1999 0.005 - - - - - - 02/24/1999 0.009 - - - - - - 02/25/1999 0.012 - - - - - - 02/26/1999 0.008 - - - - - - 02/27/1999 < 0.001 - - - - - - 02/28/1999 < 0.001 - - - - - - 08/18/1999 - 0.015 0.069 0.024 0.006 - - 08/19/1999 - 0.012 0.068 0.024 - - - 08/20/1999 - 0.023 0.070 0.024 - 0.120 - 08/21/1999 - 0.022 0.068 0.024 - - - 08/22/1999 - 0.022 0.068 0.024 - - - 08/23/1999 - 0.025 0.068 0.024 0.006 - - 08/24/1999 - 0.016 0.067 0.023 0.020 - - Page 16 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/31/2000 - < 0.002 0.026 0.009 - - - 02/01/2000 - 0.011 0.026 0.028 - - - 02/02/2000 - 0.028 0.026 0.009 0.006 - - 02/03/2000 - 0.008 0.027 0.009 - - - 02/04/2000 - 0.006 0.027 0.009 0.005 0.109 - 02/05/2000 - < 0.002 0.027 0.009 - - - 02/06/2000 - < 0.002 0.027 0.009 - - - 07/26/2000 - < 0.0057 0.055 0.019 - - - 07/27/2000 - 0.019 0.055 0.019 - - - 07/28/2000 - 0.0088 0.053 0.018 0.004 0.108 - 07/29/2000 - < 0.0088 0.055 0.019 - - - 07/30/2000 - < 0.0076 0.055 0.019 - - - 07/31/2000 - < 0.0152 0.055 0.019 0.006 - - 08/01/2000 - < 0.0141 0.055 0.019 0.005 - - 12/11/2000 - 0.0143 0.025 0.020 0.005 - - 12/12/2000 - 0.0092 0.025 0.020 0.005 - - 12/13/2000 - < 0.0120 0.025 0.020 - - - 12/14/2000 - < 0.0087 0.025 0.020 - - - 12/15/2000 - 0.0120 0.025 0.020 0.005 - - 12/16/2000 - < 0.0036 0.025 0.020 - - - 12/17/2000 - < 0.0036 0.025 0.020 - - - 08/26/2001 - 0.017 0.06 0.021 0.006 - - 08/27/2001 - <0.0096 0.06 0.021 0.005 - 0.021 08/28/2001 - <0.0085 0.06 0.021 - - - 08/29/2001 - <0.0094 0.059 0.020 0.005 - 0.021 08/30/2001 - <0.0123 0.06 0.021 0.005 - - 08/31/2001 - <0.005 0.059 0.020 - - - 11/25/2001 - <0.0044 - - - - - 11/26/2001 - <0.0119 0.024 0.02 0.005 - - 11/27/2001 - 0.0137 0.023 0.019 0.007 - - 11/28/2001 - <0.0089 0.022 0.019 0.006 - - 11/29/2001 - 0.0132 0.024 0.02 0.007 - - 11/30/2001 - < 0.0043 0.024 0.02 - - - 12/09/2001 - <0.0042 - - - - - 12/10/2001 - <0.0042 - - - - - 12/11/2001 - <0.0104 - - - - - 12/12/2001 - 0.0128 0.024 0.02 0.008 - - 12/13/2001 - <0.0088 0.024 0.02 - - - 12/14/2001 - 0.0134 0.024 0.02 0.007 - - Page 17 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/02/2002 - < 0.0079 0.023 0.02 0.006 - - 01/03/2002 - < 0.0042 0.023 0.014 - - - 01/04/2002 - 0.0124 0.024 0.014 0.009 - - 01/05/2002 - < 0.0042 - - - - - 01/06/2002 - < 0.0042 - - - - - 01/07/2002 - < 0.0089 0.024 0.014 0.006 - - 02/24/2002 - < 0.004 - - - - - 02/25/2002 - < 0.004 0.023 0.023 - - - 02/26/2002 - 0.0143 0.023 0.023 0.007 - - 02/27/2002 - < 0.0041 0.023 0.023 - - - 02/28/2002 - < 0.0041 0.024 0.008 - - - 03/01/2002 - < 0.0041 0.024 0.008 - - - 05/05/2002 - - - - - - - 05/06/2002 - - 0.058 0.02 0.014 - - 05/07/2002 - - 0.058 0.02 0.015 - - 05/08/2002 - - 0.056 0.019 - - - 05/09/2002 - - 0.057 0.02 0.014 - - 05/10/2002 - - 0.056 0.019 - - - 08/04/2002 - <0.0058 - - - - - 08/05/2002 - <0.0058 0.053 0.018 - - 0.025 08/06/2002 - 0.0092 0.053 0.018 - - - 08/07/2002 - <0.0107 0.055 0.019 0.007 - - 08/08/2002 - <0.0061 0.055 0.019 - - - 08/09/2002 - 0.0152 0.054 0.018 0.008 - - 10/06/2002 - <0.00497 - - - - - 10/07/2002 - 0.0153 0.054 0.018 0.009 - - 10/08/2002 - <0.0092 0.054 0.018 0.007 - - 10/09/2002 - 0.0124 0.053 0.018 0.009 - - 10/10/2002 - 0.0134 0.054 0.018 0.009 - - 10/11/2002 - <0.0042 0.054 0.018 - - - 01/12/2003 - <0.0035 - - - - - 01/13/2003 - <0.006 0.025 0.019 0.009 - - 01/14/2003 - <0.0118 0.026 0.020 - - 01/15/2003 - <0.0063 0.026 0.020 0.009 - - 01/16/2003 - <0.0034 0.026 0.020 - - 01/17/2003 - <0.0034 0.026 0.009 - - - 04/06/2003 - <0.0073 - - - - - 04/07/2003 - <0.0189 - 0.021 - - - 04/08/2003 - <0.0117 - 0.021 - - - 04/09/2003 - <0.0139 - 0.021 0.016 - - 04/10/2003 - <0.0113 - 0.021 0.018 - - 04/11/2003 - <0.0073 - 0.022 - - - Page 18 of 99
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 06/15/2003 - < 0.0045 - - - - - 06/16/2003 - < 0.0037 0.057 0.020 - - 0.022 06/17/2003 - < 0.0048 0.041 0.014 - - 0.024 06/18/2003 - < 0.0048 0.041 0.014 - - 0.024 06/19/2003 - < 0.0085 0.058 0.020 - - 0.025 06/20/2003 - < 0.0048 0.058 0.020 - - 0.025 08/03/2003 - <0.0050 - - - - - 08/04/2003 - <0.0050 0.058 0.020 - - - 08/05/2003 - <0.0051 0.057 0.020 - - 0.025 08/06/2003 - <0.0084 0.057 0.020 - - 0.025 08/07/2003 - 0.0129 0.057 0.020 - - 0.024 08/08/2003 - 0.0153 0.057 0.020 0.009 - - 10/05/2003 - <0.0043 0.057 0.020 - - - 10/06/2003 - <0.0043 0.057 0.020 - - 0.025 10/07/2003 - <0.0090 0.057 0.020 - - 0.025 10/08/2003 - <0.0106 0.057 0.020 - - 0.025 10/09/2003 - 0.0181 0.026 0.022 - - 0.025 10/10/2003 - 0.0183 0.026 0.024 0.009 - - 02/01/2004 - 0.0093 0.027 0.009 - - - 02/02/2004 - <0.0034 0.026 0.009 - - - 02/03/2004 - <0.0034 0.026 0.009 - - - 02/04/2004 - 0.0124 0.026 0.009 0.009 - - 02/05/2004 - <0.0034 0.026 0.009 - - - 02/06/2004 - 0.0105 0.026 0.009 0.010 - - 05/04/2004 - <0.0123 0.026 0.019 - - 0.025 05/05/2004 - <0.0144 0.026 0.014 0.009 - 0.025 05/06/2004 - <0.0146 0.037 0.013 - - 0.025 05/07/2004 - 0.0227 0.058 0.020 0.009 - 0.025 05/08/2004 - 0.016 0.060 0.021 - - - 05/09/2004 - <0.0104 0.058 0.020 - - - 07/04/2004 - 0.0217 0.057 0.019 - - - 07/05/2004 - <0.0085 0.057 0.020 0.009 - - 07/06/2004 - <0.0077 0.058 0.020 - - 0.031 07/07/2004 - 0.0252 0.056 0.019 - - 0.031 07/08/2004 - 0.0223 0.057 0.019 0.009 - - 07/09/2004 - 0.0182 0.057 0.020 0.009 - - Page 19 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M Hypochlorit mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L e TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L EO/PO TRC 11/07/2004 - <0.0187 0.000 0.014 - - - - - 11/08/2004 - <0.0192 0.047 0.030 - - - - - 11/09/2004 - <0.0233 0.048 0.016 - - 0.041 - - 11/10/2004 - <0.0149 0.047 0.016 - - 0.041 - - 11/11/2004 - <0.0149 0.049 0.017 - - 0.043 - - 11/12/2004 - <0.0253 0.048 0.017 - - 0.042 - - 02/06/2005 - <0.0042 0.028 0.010 - - - - - 02/07/2005 - <0.0116 0.028 0.010 - - - 0.007 - 02/08/2005 - <0.0080 0.028 0.010 - - - - - 02/09/2005 - 0.0199 0.028 0.010 - - - - - 02/10/2005 - <0.0042 0.028 0.010 - - - - - 02/11/2005 - 0.0155 0.028 0.010 - - - 0.007 - 06/05/2005 - 0.0063 - - - - - - - 06/06/2005 - 0.0043 - - - - - - 0.037 06/07/2005 - 0.0103 - - - - - - 0.037 06/08/2005 - 0.0295 - - - - - - 0.037 06/09/2005 - 0.0129 - - - - - - - 06/10/2005 - 0.0184 - - - - - - - 07/17/2005 - 0.0109 0.026 0.009 - - - - - 07/18/2005 - 0.0150 0.026 0.009 - - - - 0.036 07/19/2005 - 0.0163 0.026 0.009 - - - - 0.036 07/20/2005 - 0.0209 0.026 0.009 - - - 0.014 0.036 07/21/2005 - 0.0242 0.026 0.009 - - - - - 07/22/2005 - 0.0238 0.054 0.018 - - - 0.014 - 10/30/2005 - 0.0068 - - - - - - - 10/31/2005 - 0.0112 - - - - - - - 11/01/2005 - 0.0104 - - - - - - 0.035 11/02/2005 - 0.0104 - - - - - - 0.036 11/03/2005 - 0.0117 - - - - - - 0.036 11/04/2005 - 0.0165 - - - - - - 0.035 11/14/2005 - 0.0274 - - - - - - - 11/15/2005 - 0.0256 - - - - - - - 11/16/2005 - 0.0234 - - - - - - - 11/17/2005 - 0.0231 - - - - - - - 11/18/2005 - 0.0200 - - - - - - - 11/19/2005 - 0.0116 - - - - - - - Page 20 of 99
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M MSW Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L 101 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L TRC EO/PO Phosphate 11/12/2006 - 0.0055 - - - - - - - - 11/13/2006 - 0.0068 - - - - - - 0.037 - 11/14/2006 - 0.0143 - - - - - - 0.037 - 11/15/2006 - 0.0068 - - - - - - 0.037 - 11/16/2006 - 0.0267 - - - - - - 0.037 - 11/17/2006 - 0.0222 - - - - - - - - 11/26/2006 - 0.0188 - - - - - - - - 11/27/2006 - 0.0138 - - - - - - - - 11/28/2006 - 0.0120 - - - - - - - - 11/29/2006 - 0.0288 - - - - - - - - 11/30/2006 - 0.0376 - - - - - - - - 12/01/2006 - 0.0187 - - - - - - - - 05/28/07 - - - - - - - - - 0.015 05/29/07 - - - - - - - - 0.036 0.015 05/30/07 - 0.0084 - - - - - 0.017 0.036 0.015 05/31/07 - 0.0103 - - - - - - 0.036 0.015 06/01/07 - 0.0164 - - - - - 0.017 0.036 0.015 06/02/07 - 0.0305 - - - - - - - 0.015 12/02/07 - 0.0241 - - - - - - - - 12/03/07 - 0.0128 - - - - - - - - 12/04/07 - 0.0238 - - - - - - - - 12/05/07 - 0.0158 - - - - - - - - 12/06/07 - 0.0162 - - - - - - - - 12/07/07 - 0.0175 - - - - - - - 04/13/08 - 0.0039 - - - - - - - - 04/14/08 - 0.0124 - - - - - - - - 04/15/08 - 0.0229 - - - - - - - - 04/16/08 - 0.0143 - - - - - - - - 04/17/08 - 0.0120 - - - - - - - - 04/18/08 - 0.0149 - - - - - - - - 10/26/08 - 0.0260 - - - - - - - - 10/27/08 - 0.0151 - - - - - 0.017 - - 10/28/08 - 0.0172 - - - - - - 0.041 - 10/29/08 - 0.0154 - - - - - 0.018 0.041 0.030 10/30/08 - - - - - - - - 0.041 0.030 10/31/08 - 0.0086 - - - - - - 0.041 0.030 Page 21 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg/L Quat mg/L TRC Phosph EO/PO Quat Phosphate ate 02/08/09 - 0.0197 - - - - - 0.017 - - - 02/09/09 - 0.0237 - - - - - 0.017 - - - 02/10/09 - 0.0104 - - - - - 0.021 - - - 02/11/09 - 0.0155 - - - - - 0.017 - - - 02/12/09 - 0.0106 - - - - - 0.017 - - - 02/13/09 - - - - - - - - - - - 05/10/09 - 0.0129 - - - - - - - - - 05/11/09 - 0.0415 - - - - - - - 0.0446 - 05/12/09 - 0.0053 - - - - - - - 0.0396 - 05/13/09 - 0.0049 - - - - - - - 0.0396 - 05/14/09 - <0.0141 - - - - - - - 0.0397 - 05/15/09 - <0.0160 - - - - - - - - - 11/15/09 - 0.025 - - - - - - - - - 11/16/09 - 0.0152 - - - - - - - - - 11/17/09 - 0.0255 - - - - - - - - - 11/18/09 - 0.0306 - - - - - - - - - 11/19/09 - 0.0204 - - - - - - - - 11/20/09 - 0.0093 - - - - - - - - - 05/09/10 - 0.0192 - - - - - - - - - 05/10/10 - 0.0055 - - - - - - - - - 05/11/10 - 0.0100 - - - - - - 0.039 - - 05/12/10 - 0.0171 - - - - - - 0.039 - - 05/13/10 - 0.0041 - - - - - 0.039 - - 05/14/10 - 0.0099 - - - - - - 0.039 - - Page 22 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Floguard Hypo- mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly- DMAD Azole Quat mg/L mg/L Quat mg/L mg/L mg/L Phos- mer EO/PO Quat Phos- Phosphate TRC phate phate 10/31/10 - - - - - - - - - - - - 11/01/10 - 0.0122 - - - - - - - - - - 11/02/10 - 0.0112 - - - - - - - - - - 11/03/10 - 0.0163 - - - - - - - - - - 11/04/10 - 0.0107 - - - - - - - - - - 11/05/10 - 0.0132 - - - - - - - - - - 05/01/2011 - - - - - - - - - - - - 05/02/2011 - - - - - - - - 0.04 - - - 05/03/2011 - - - - - - - - 0.04 - - - 05/04/2011 - 0.0155 - - - - - - 0.04 - - - 05/05/2011 - 0.0179 - - - - - - 0.04 - - - 05/06/2011 - 0.0089 - - - - - - - - - - 11/06/2011 - 0.0168 - - - - - - - - - - 11/07/2011 - 0.0225 - - - - - - - - - - 11/08/2011 - 0.0141 - - - - - - - - - - 11/09/2011 - 0.0239 - - - - - - - - - - 11/10/2011 - 0.0242 - - - - - - - - - - 11/11/2011 - 0.0231 - - - - - - - - - - 05/06/2012 - - - - - - - - - - - - 05/07/2012 - - - - - - - - - - - - 05/08/2012 - - - - - - - - 0.041 - - - 05/09/2012 - 0.0145 - - - - - - 0.041 - - - 05/10/2012 - 0.0298 - - - - - - 0.041 - - - 05/11/2012 - 0.0174 - - - - - - - - - - 08/12/2012 - - - - - - - - - - - 0.029 08/13/2012 - 0.0256 - - - - - 0.028 0.037 - - 0.029 08/14/2012 - 0.0209 - - - - - - 0.037 - - 0.029 08/15/2012 - 0.0279 - - - - - 0.028 - - - 0.029 08/16/2012 - 0.0076 - - - - - - - - - 0.029 08/17/2012 - 0.0446 - - - - - - - - - 0.032 Page 23 of 99
Sequoyah Nuclear Plant Biomonitoring August 14 - 21, 2012 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 24 of 99
BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of_l_ Page 25 of 99 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 3 51 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): _ _ _ _ _ __ General Comments: Facility Sampled: Sequoyah NP 28801 Toofo.. J~"'ti~J q .,_ o"+-FfIt 4 t o ~o o~ 17c.;a,/ NPDES Number: TN0026450 Phone: 828-350-9364 s ryl-c.s fr~M ~Af-tkf... _, r o I.,Jo,
.Fax: 828-350-9368 Container Field Identification I Number& Flow Rain Event?
Grab/Comp. Collection Date/Time Sample Description (MGD) (Mark as Appropriate) If Yes, Start End Yes No Trace Inches
~IJ.,_tJ. 8"1]--12 SQN-101-TOX Comp VCt.tr {!)J'"tJ~e:r 2(2.5gal)
X SQN-INT-TOX Comp
,_,,_ ,,_ E' r-IJ-1').
1(2.5 gal) o 7PJ' e' Relinquished By (Signature): Date/Time SoN\~ 0 08
- r~- rL 1$'/0 E.\
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page __12_( Whole Efflue( )ample Receipt Log
*Sample temperature performed using Sample Receiving Thermometer: SN 6338 Page 26 of 99 Date Time Received Received *Sample Project Sample 0
Sample name and description State Comments Received Received by from Temp. ( C) number number 08-13-12 1540 J. Sumner Sonic Delivery 0.8,0.7 8207 120813 .01 TVA I SQN Outfall101 TN 08-13-12 1540 J. Sumner Sonic Delivery 0.9 8207 120813 .02 TVA/ SQN Intake TN SOP G4 -Exhibit G4.2, revision 01-03-12
BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of_l_ Page 27 of 99 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 3 51 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): _ _ _ _ _ __ General Comments: Facility Sampled: Sequoyah NP 28801 To{) k J 'i 11fJit- > o-f+ c; r 0 v+ A II ~T 'I> 0 NPDES Number: TN0026450 Phone: 828-350-9364 CiAo/ ::t';'\ fiO*kl- a Y CJ 0. Collected By: f) v) r/1). H." 4J t.r Fax: 828-350-9368 fvWir ~ Container Field Identification I Number & Flow Grab/Comp. Collection Date/Time Rain Event? Sample Description Volume (MGD) (Mark as Appropriate) Collected Start End No Trace
'&""l"l'IJ. -rr~t.l SQN-101-TOX Comp (P'7(J0 'E~ 0 bOO t:,"t"' 2(2.5gal)
X f-lt.t"' '}., Y-l)"'IJ-SQN-INT-TOX Comp o7 J-o -c:"t" o ~.lo E.'t" 1(2.5 gal) Relinquished By (Signature): Date/Time
/0.,:;30 E-r Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Whole Efflue{/-~':::ample Receipt Log Page J.:1,b
*Sample temperature performed using Sample Receiving Thermometer: SN 6338 Page 28 of 99 Date Time Received Received *Sample Project Sample Sample name and description State Comments Received Received by from Temp. (°C) number number 08-15-12 0945 K.Keenan Fed- Ex 1.3 8215 120815 .01 CORR ERI- AAF McQuay International NC 08-15-12 0945 K.Keenan Fed- Ex 0.7 8216 120815 .02 Statesville Analytical- Cleveland WWTP NC 08-15-12 0945 K.Keenan Fed- Ex 5.5 8217 120815 .03 United Water- Enfield WWTP NC 08-15-12 0945 K.Keenan Fed- Ex 5.5 8218 120815 .04 United Water- Scotland Neck WWTP NC 08-15-12 0945 K.Keenan Fed- Ex 0.3 8219 120815 .05 Craven County Wood Energy NC 08-15-12 0945 K.Keenan Fed- Ex 0.7 8220 120815 .06 Duke Energy- McGuire NS- Outfall 002 NC 08-15-12 0945 K.Keenan Fed- Ex 0.6 8221 120815 .07 Progress Energy- Shearon Harris E & E NC 08-15-12 0945 K.Keenan Fed- Ex 0.5 8222 120815 .08 Carolina Beach WWTP NC 08-15-12 0945 K.Keenan UPS 2.0 8223 120815 .09 Morehead City WWTP NC 08-15-12 1553 J. Sumner TVA Courier 1.9/1.9 8207 120815 .10 TVA- SQN -101 TN 08-15-12 1553 J. Sumner TVA Courier 2.0 8207 120815 .11 TVA- SQN - Intake TN SOP G4 -Exhibit G4.2, revision 01-03-12
BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of_l_ Page 29 of 99 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 3 51 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): _ _ _ _ _ __ General Comments: Facility Sampled: Sequoyah NP 28801 hDk S'~lt.--J frl),...., o,.,fA/1 ctr Of 6
~ ~~
NPDES Number: TN0026450 Phone: 828-350-9364 fr0 .,.-.., J&nf'<;/(t.. q f- o ( .3ot Fax: 828-350-9368 Container Field Identification I Number & Flow Grab/Comp. Collection Date/Time Rain Event? Sample Description Volume (MGD) (Mark as Appropriate) Collected If Yes, Start No Inches
-n-rJ.. ?-
SQN-101-TOX Comp 0 ?oo e.-t 0'10(90 E.T 2(2.5gal) t-1(, -tJ-SQN-INT-TOX Comp 1(2.5 gal) ~ II tl7{J £( Relinquished By (Signature): Date/Time 0 * \\- \1.. Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Whole Effluer )ample Receipt Log Page llS
*Sample temperature performed using Sample Receiving Thermometer: SN 6338 Page 30 of 99 Date Time Received Received *Sample Project Sample 0
Sample name and description State Comments Received Received by from Temp. { C) number number 08-17-12 0950 K.Keenan Fed- Ex 0.6 8215 120817 .01 CORR ERI - AAF McQuay International NC 08-17-12 0950 K.Keenan Fed- Ex 0.6 8216 120817 .02 Statesville Analytical - Cleveland WWTP NC 08-17-12 0950 K.Keenan Fed- Ex 2.0 8217 120817 .03 United Water- Enfield WWTP NC 08-17-12 0950 K.Keenan Fed- Ex 2.0 8218 120817 .04 United Water- Scotland Neck WWTP NC 08-17-12 0950 K.Keenan Fed- Ex 0.7 8219 120817 .05 Craven County Wood Energy NC 08-17-12 1130 J. Sumner Murphy Courier 0.8 8226 120817 .06 Duke Energy- McGuire NS- Upper Composite NC 08-17-12 1130 J. Sumner Murphy Courier 0.8 8226 120817 .07 Duke Energy- McGuire NS- Lower Composite NC 08-17-12 1338 J. Sumner TVA Courier 2.2/2.2 8207 120817 .08 TVA- SQN- Outfall101 TN 08-17-12 1338 J. Sumner TVA Courier 2.4 8207 120817 .09 TVA- SQN -Intake TN SOP G4 -Exhibit G4.2, revision 01-03-12
s Page 1 of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: Tennessee Valley Authority County: Rhea Facility: Seguoyah Nuclear Plant Outfall: 101 NPDES #: TN0020168 Project#: _o~z.cr\---...._______ Dilution preparation information: Comments: Dilution prep (%) 10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information: Organism age: 1.o ~\)t(~ 0\-b Randomizing template: "ec..to\A) Date and times organisms 0~* \'\*\'1.. \\o()() *. *Incubator ntunber and were born between: shelf location: ~~ Organism source: Artemia CHM number: t.R"' \Qt;l.. A~><.~~ Pe oa-*~""L.. Drying information for weight determination: Transfer bowl information: pH= '*\.DO s.u. Date I Time in oven: o&* '2.\* n .. \-..00 Temperature = 1.S. \ oc Initial oven temperature: lob' c.. Average transfer volume: Date I Time out of oven: 0!"1.1..*\~
- o. \L.o~A
~
Final oven temperature: ~C) *c.. Total drying time: 1-\l*Jo\wtS. Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used 5 6 7 l Page 31 of 99 SOP AT20 -Exhibit AT20 .3, revision 07-01-12
s Page2 of6 Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant, OutfalllOl, Non-treated Date: 0&* I q
- l ,_
Survival and Growth Data Day t;U.NT.KUL 10.:~%_ 21.6% A B c D E F G H I J K L 0
/0 f() 'D /Q /0 /0 IC 10 /0 10 10 ID 1
/0 /Q 10 10 /Q 10 10 It) IO 10 IO 10 2
/0 to IO 10 10 /() lO /0 10 IO to to 3
}0 /0 {(J 10 10 IC IO 10 10 1\) 10 10 4
10 /0 lO IO It> 10 10 10 tD IQ 10 ID 5 10 10 10 10 10 10 10 10 IC /() I(J 10 6
/0 IC 10 ID ID IC 10 IQ /C 10 10 10 7
/O ID /0 10 /Q 10 tD I(J 10 10 /Q 10 A= Pan weight (mg) "
Tray color code:: \1?\..\- 'P:.,.,k Analyst: M._ t4.4-l t3.ss t4.z4 13.24 14.1o 13.Sc. 13,\.8 '3.3S t4.zs IZ.!1S IS.C-) 14.~1 Date: QS*aC.*'2 B = Pan + Larvae weight (mg) Analyst: ~lJ!,
~*2.'1.f'l..
't,C).'30 \'1.7 3 z.o.l' IS.6S" l't.'l5 zo.ss -zn.S'i ,q.t7 1.0.3'3 r~S'f 2.1 .1'3 "ZJ .8.3 Date:
C = Larvae weight (mg) = B- A Hand calculated. Analyst: A._ s.a~ C..\S s.~,. s."\"\ s.!:.~ ~ ...'\ -..&~ ..."\4\ ... G& S..4f.~ Co.1.Co ~.&Co
~
Weight per initial number oflarvae (mg)
...J'
= C I Initial number of larvae '
'Q'- AI ~
t..;~ c;~ f'\1
~
Hand calculated. r>>"' -b ct y~ ~ "- \o ~ '0 Analyst: )l[...,... o* " o* o' 0" t:J. o* o*"" t)" ()'
~* t:l f;J Average weight per Percent reduction initial number of from control (%) o.s&'-t 0. 'o~S> - &, ~ 7. o.~*t'o ... ~ .1. 7, larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk = sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: SOP AT20- Exhibit AT20.3, revision 07-01-12 Page 32 of 99 ' ,.- **;>.
!ET OEnvlranmentaiTestlng~udons.fne. Page 3 of6 Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant, OutfalllOl, Non-treated Date: o&* t'-\.*1'-- Survival and Growth Data Day 43.2% 86.4% 100% M N 0 p .Q R s T u v w X 0 I() 10 to /0 IC /() 10 I() /D /0 /(:) IO 1 10 10 10 10 10 IO 10 10 10 to 10 I() 2 ID 10 10 IO to lD JO 10 /() 10 /() 10 3 (tJ 10 10 /0 /Q 10 10 10 I(;) to tO /(J 4 ({J IQ /Q IO /0 IO /Q ID /0 IO IO co 5
/D /() 10 10 10 10 10 10 10 ID 10 I()
6
/tl to ID 10 IC ((J /\) I{) It) /Q /Q 10 7 1.-Y'\
/C lb t(J 10 lb IC /0 10 /() 10 IO IO A = Pan weight (mg) "'
Tray color code:: ~Jr 'P:l\1.. Analyst: _t_ \3.So *'3.c-o r4a~ *4.3, lZ..tA S.S1 3,:}-t\ lt.~'L t'3.c;o t3.1o 1:!..'31 r4:5o Date: QS*~*Il. B = Pan + Larvae weight (mg) Analyst: ~ 1Cf.'5l. 11.1.3 ,q_,-z. z.o.'"IS IS."7S 11-'fZ l't. 'fZ. 'Z.I.'I'2. l"i~'Z- -zo.'f'J *~."'l "'Z..O. Z.l Date: Otl*1:7*1't. C = Larvae weight (mg) = B -A Hand c~lculated. s.&1.. s.-.~ '-\ . &1 t..l'-\. Co. l'-\ ~.~~ s.c.:!t \..4\0 s.~'2. c..'"l~ s.ofo 5.1\ Analyst: d~ Weight per initial number oflarvae (mg)
= C I Initial number of larvae Hand calculated. A .,...._ 4V r;f
,., ~I\ ~ 'C)
~
9,., t:~"" ~
"0 ~'V A'? y~
o*
, ~
o*':> o*
... \0 'D Analyst: o* t:1 tl. o' cr ~- b.
Average weight per
\. ' t::l '0'"
Percent reduction initial number of from control (%) , o.So1. ~.1.7. O.bOio -~.t7. o.~1\ 4.47. Iarvae_im2) Comment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: SOP AT20- Exhibit AT20.3, revision 07-01~12 Page 33 of 99
iET
~ Envln>nrn<ntaiTestlngSolutions,lnc. Page 4 of 6 Day 0
1 10 tO 2 lO /0 /0 3 (0 I() IO 4 10 10 /0 5 10 10 10 6 to ft) 10 7 ID t(J A= Pan weight (mg) Tray color code:: }t~ £ale. Analyst: _ _-!MJa~~------ Date: DS*Ol..*\1. 13.84 14.19 14.tt 14.24 B = Pan + Larvae weight (mg) Analyst: OU 1.l. Date: Oi*z.?.it. ttt.Z.l 1.0."17 -z.l." C = Larvae weight (mg) =B -A ' Hand calculated. Y S"!t1 c&,\.C\ ~.~\ tc..t1 Analyst: g""""'_,___ Weight per initial number oflarvae (mg)
= C I Initial number of larvae Hand calculated.A{
Analyst: (:;;J- _ Average weight per Percent reduction initial number of from control(%) o.~&fo larvae Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: SOP AT20 -Exhibit AT20.3, revision 07-01-12 Page 34 of 99
TVA I Sequoyah Nuclear Plant, Outfall101 Non-treated Page 35 of 99 August 14-21, 2012
** Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc. Project number: 8207 Not for Compliance Assessment, Internal Laboratory QC Concentration(%) Rep6cate Initial number of Final number of larvae A= Pan weight (mg) B =Pan+ Larvae Larvae weight (mg) Weight I Surviving Mean weight I Coefficient of variation Weight I Initial number Mean survival Mean weight I Initial Coefficient of Percent reduction from larvae weight(mg) =A-B number oflarvae (mg) Surviving number of (Meanweightprrsurviring of larvae (mg) (%) number of larvae variation (Mean weight control(%)
larvae(mg) numbororlarva*)(%) (mg) perinitialnumberoriarvae) (%l_ A IO IO I4.47 20.30 5.83 0.583 0.583 Control B IO IO 13.58 I9.73 6.15 0.6I5 0.615 0.584 5.1 100.0 0.584 5.1 Not applicable c 10 10 I4.24 20.I6 5.92 0.592 0.592 D IO IO 13.24 18.68 5.44 0.544 0.544 E 10 10 14.10 19.45 5.35 0.535 0.535 F 10 10 13.86 20.55 6.69 0.669 0.669 10.8% 0.635 10.7 100.0 0.635 10.7 -8.8 G IO IO 13.68 20.54 6.86 0.686 0.686 H IO IO 13.38 19.87 6.49 0.649 0.649 I IO IO I4.25 20.33 6.08 0.608 0.608 J 10 10 I2.95 18.54 5.59 0.559 0.559 21.6% 0.620 8.5 100.0 0.620 8.5 -6.2 K IO 10 15.67 21.93 6.26 0.626 0.626 L 10 10 14.97 21.83 6.86 0.686 0.686 M IO IO 13.50 I9.32 5.82 0.582 0.582 N 10 10 13.60 19.23 5.63 0.563 0.563 43.2% 0.562 9.6 100.0 0.562 9.6 3.8 0 IO IO I4.75 19.62 4.87 0.487 0.487 p IO 10 14.3I 20.45 6.14 0.614 0.6I4 Q IO IO I2.64 I8.78 6.I4 0.6I4 0.6I4 R IO 10 13.87 I9.42 5.55 0.555 0.555 86.4% 0.606 I0.3 100.0 0.606 10.3 -3.8 s IO 10 13.79 19.42 5.63 0.563 0.563 T IO IO I4.52 21.42 6.90 0.690 0.690 u 10 10 13.50 18.82 5.32 0.532 0.532 100% v IO IO I3.70 20.43 6.73 0.673 0.673 0.571 I2.9 100.0 0.571 12.9 2.2 w IO IO 13.37 I8.43 5.06 0.506 0.506 X IO IO I4.50 20.2I 5.7I 0.57I 0.57I y IO 8 13.84 I9.21 5.37 0.67I 0.537 100% Intake z IO IO I4.78 20.47 5.69 0.569 0.620 Il.2 0.569 95.0 0.586 11.7 -0.4 AA IO IO I4.71 20.22 5.5I 0.55I 0.55I BB IO 10 I4.24 21.11 6.87 0.687 0.687 Outfall101: MSD= Minimum Significant Difference Dunnett's MSD value: 0.0994 PMSD= Percent Minimum Significant Difference PMSD: 17.0 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Intake: Dunnett's MSD.value: 0.0726 Lower PMSD bound determined by USEPA (I Oth percentile) = I2%. PMSD: 12.4 Upper PMSD bound determined by USEP A (90th percentile) = 30%. Lower and upper PMSD bounds were determined from the I Oth and 90th percentile, respectively, of PMSD data from EPA's WET InterlaboratOiy Variability Study (USEPA, 2001 a; USEPA, 200 I b). USEPA. 200Ia, 200Ib. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-82I-B-OI-004 and EPA-821-B-OI-005. US Environmental Protection Agency, Cincinnati, OH. File: sqn101_081412data.xlsx Entered by: J. Sumner Reviewed by:--?
TVA I Sequoyah Nuclear Plant, Outfall101 Non-treated August 14-21, 2012 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 8/14/2012 Test ID: PpFRCR Sample ID: TVA I SQN 101 End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Cone-% 1 2 3 4 D-Control 0.5830 0.6150 0.5920 0.5440 10.8 0.5350 0.6690 0.6860 0.6490 21.6 0.6080 0.5590 0.6260 0.6860 43.2 0.5820 0.5630 0.4870 0.6140 86.4 0.6140 0.5550 0.5630 0.6900 100 0.5320 0.6730 0.5060 0.5710 Intake 0.5370 0.5690 0.5510 0.6870 Transform: Untransformed 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5835 1.0000 0.5835 0.5440 0.6150 5.069 4 0.6127 1.0000 10.8 0.6348 1.0878 0.6348 0.5350 0.6860 10.744 4 -1.242 2.410 0.0994 0.6127 1.0000 21.6 0.6198 1.0621 0.6198 0.5590 0.6860 8.465 4 -0.879 2.410 0.0994 0.6127 1.0000 43.2 0.5615 0.9623 0.5615 0.4870 0.6140 9.607 4 0.533 2.410 0.0994 0.5835 0.9524 86.4 0.6055 1.0377 0.6055 0.5550 0.6900 10.256 4 -0.533 2.410 0.0994 0.5835 0.9524 100 0.5705 0.9777 0.5705 0.5060 0.6730 12.860 4 0.315 2.410 0.0994 0.5705 0.9312 Intake 0.5860 1.0043 0.5860 0.5370 0.6870 11.706 4 Auxilia!1 Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98195 0.884 0.03805 -0.4693
=
Bartlett's Test indicates egual variances {[! 0.82l 2.21405 15.0863
~~othesis Test {1-tail, 0.051 NOEC LOEC ChV TU MSDu MSD~ MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.09943 0.17041 0.00332 0.0034 0.4589 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point % so 95% CL{Ex~l Skew IC05 87.934 IC10 >100 IC15 >100 1.0 -r-------------, IC20 >100 0.9 IC25 >100 0.8 IC40 >100
>100 0.7 IC50 0.6 Q) 1!1 0.5
&. 0.4
~ 0.3 0.2 Q1 ~
0.0~-**** ****,*'
-0.1 *,.'
-0.2 +-r--r--.--.-..,.-.,......,r-r--.--r--r-r-r--r-1 0 50 100 150 Dose%
Dose-Response Plot 0.8 0.7 0.6 l 0.5 1-tail, 0.05 level e 0.4 (!) of significance
~
~ 0.3 0.2 0.1 0
- g CX!
0
<<:! N (I)
'<I:
CD 0
~
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c: 0 N v IX) .19 u .E 0 File: sqn101_081412data.xlsx Entered by: J. Sumner Reviewed by:-+ Page 36 of 99
TVA I Sequoyah Nuclear Plant, Intake Non-treated August 14-21, 2012 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 8/14/2012 Test ID: PpFRCR Sample ID: TVA I SQN 101 -Intake End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Cone-% 1 2 3 4 D-Control 0.5830 0.6150 0.5920 0.5440 10.8 0.5350 0.6690 0.6860 0.6490 21.6 0.6080 0.5590 0.6260 0.6860 43.2 0.5820 0.5630 0.4870 0.6140 86.4 0.6140 0.5550 0.5630 0.6900 100 0.5320 0.6730 0.5060 0.5710 Intake 0.5370 0.5690 0.5510 0.6870 Transform: Untransformed 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.5835 1.0000 0.5835 0.5440 0.6150 5.069 4 10.8 0.6348 1.0878 0.6348 0.5350 0.6860 10.744 4 21.6 0.6198 1.0621 0.6198 0.5590 0.6860 8.465 4
. 43.2 0.5615 0.9623 0.5615 0.4870 0.6140 9.607 4 86.4 0.6055 1.0377 0.6055 0.5550 0.6900 10.256 4 100 0.5705 0.9777 0.5705 0.5060 0.6730 12.860 4 Intake 0.5860 1.0043 0.5860 0.5370 0.6870 11.706 4 -0.067 1.943 0.0726 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.88547 0.749 1.36005 1.98359 F-Test indicates egual variances (p = 0.20) 5.37752 47.4683 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.07258 0.12439 1.2E-05 0.00279 0.94881 1' 6 Treatments vs D-Control Dose-Response Plot 0.7 0.6 1-tail, 0.05 level J 0.5 0
of significance (5 0.4 i;' c 0.3
"' 0.2 0.1 co oo:r: 0 0
,.. <0 0 co File: sqnl01_081412data.xlsx Page 37 of 99 Reviewed by: T Entered by: J. Sumner
Page 5 of6 Species: Pimeohales promelas Date: _ _o=-t_*l_'-....;;..l*_.;\_.;1...=--- Ciient: TVA I Seguoyab Nuclear Plant. OutfalllOl. Non-treated CONTROL Non-treated 10.8% I 21.6% I I 43.2% li 86.4% li 100% li 100% Intake
*Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
- r. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet SOP AT20- Exhibit AT20.3, revision 07-01-12 li Page 38 of 99
s Page 6 of6 Analyst Concen- Parameter tration CONTROL 1- ~ 100% I I I 100% Intake I I *Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet I Page 39 of 99 SOP AT20- Exhibit AT20.3, revision 07-01-12
**~****'":
TVA I Sequoyah Nuclear Plant, Outfa11101 - Non-treated August 14-21, 2012 Page 40 of 99 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number: _ _ _ _ _ _82......;0_7 Concentration Control 10.8% 21.6% 43.2% 86.4% 100% 100% Intake File: sqn1 01_081412chem.xls Entered by: J. Sumner Reviewed b y : +
!ETS Page 1 of7 0 Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: Tennessee Valley Authority County: Hamilton Facility: Seguoyah Nuclear Plant Outfall: 101 NPDES #: TN0026450 Project#: --=4-=-1...=<0=------- Dilution preparation information: Comments: Dilution prep(%) 10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism source information: Test information: Organism age: < 24-hours old Randomizing template color: 6-()'-,.t) Date and times organisms were born Ol*,"\* \1... 0'-LO "tt ct\lt) between: Incubator number and shelf Culture board: ot*cn- ,,_ ~ location: 'Z.e,1... Replicate number: I I 2 I 3 I 4 I 5 I 6 I 7 I 8 I 9 10 YWTbatch: o&*()t*l"'\.. Culture board cup number: \1.1 "~I "U> 1-a.\ 1\'L 11-t I~\ I lll ~ "s Transfer vessel information: I pH= .,*., \ s.u. Temperature = "1.'{:" oc Average transfer volume (mL): I 0
- 0'!.1.1.....Q Selenastrum batch: 0"1*~\*l\...
Daily renewal information: Day Date Test initiation and feeding, MHSW Samplen Analyst renewal and feeding, or batch used Outfall lOt termination time 0 2 3 4 5 6 7 Control information: Summary of test endpoints: Control-I Control-2 Acceptance criteria % of Male Adults: 01. _0_7, ~20% 7-day LCso )J0()7. %Adults having 3ra Broods: /007 1007. ;?!80% NOEC 1007. % Mortality: C7 . 0_7. ~20% LOEC )1007. Mean Offspring/Female: 3l. '2.. .Jl.~ ;?! 15.0 offspring/female ChV )1007 %CV: '-* '1.7., s. "7. <40.0% IC2s ., 1001. SOP AT11- Exhibit AT11.2, revision 07-01-12 Page 41 of 99
Page 2 of7 Species: Ceriodaphnia dubia f Client: TVA I Seguoyah Nuclear Plant. Outfall101 CONTROL-1 Survival and Reproduction Data Date: 0&
- 1'-t*l"'l.-
Replicate number I; Day* 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 () 0 0 D b 0 a Adult mortality
'- L L- L L L L L L L I 2 Young produced D. D a \..._
a () 0 (') 0 0 0 Adult mortality \..... \...... \.._ L
'- '-- L L. \._.
3 Young produced C) 0 0 0 () 0 c a 0 () I 4 Adult mortality Young produced L __c; ~
'4 s
s 5 5 L Co
~ \..
Adult mortality \..... '-- '-- L.,_
'-- \..._ L '-- '-- '--
*5 Young produced Adult mortality 'L\ \.\
\......
I()
\1.,... \C
~
L
\1....
L
\\
\..._
\\
\_\
L... 6 Young produced D b 0 0 a 0 C> 0 0 0 7 Adult mortality Young produced Total young produced 1"\ L {
-'a*
eJAj
&5
\....... L Lt. '~
\..._
*'-\ ,,
L.... L
\~
'~
L \.._
\S
--,v "l.'\ ~"b ~\ 7..i ~'\ ~-z... ~b ~,_
I l>>b !if Final Adult Mortality '- "- "- '-- . '-- ~ '- '-- \...... L.
')(... -~ ~ ~ ::>5- _25-,. }C.. )<-
X for 3rd Broods ..
)£.
Note: Adult mortahty (L- hve, D- dead), SB- spht brood (smgle brood split between two days), c_.o =carry over (offspnng earned over With adult dunng transfer). I Concentration: 7..
% Mortali *:
CONC: 10.8% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced L) () _0 0_ Q_ 0 0 0 0 _(') Adult mortality L L L L L L.. L L L l_ 2 Young produced 0 0 a 0 0 Q D {) 0 _D Adult mortality \._. L L L L L.. L L L.... L.. 3 Young produced a 0 0 0 0 0 a_ 0 0 0 Adult mortality L \. L L L L L L L L. 4 Young produced ~ *c. 4 \.{ ~ to y (., 6 _S L. L.. \.._ 5 Adult mortality Young produced \~
\...-
\L.. \\ ,,_'-- \"'L
\.._
\0 '~
'-- \.._
'1...
\...._.
)(..
L Adult mortality \..... '-- L \...._ L L L L.. '-- \...._. Young produced 0 0 0 0 0 u 0 0 0 CJ 6
\._ L Adult mortality L
,..., L- L L '-- L L \.....__
7 Young produced \Q \i li \~ \\ \\o I'-\ lS Total young produced *
~to a~ ~ ~3 ~s :!JS 3.'1 3'! ~\ ~L.
Final Adult Mortality \...... '- \...._ \. L. L L. L L.. \..._ Note: Adult mortality (L =live, D- dead), SB- split brood (single brood split between two days), CO= can over(offspring carried over with adult during transfer). Concentration:
% Mortality: 07.
Mean Offspring/Female: .3~*1
%Reduction from Control- I: -K-07.
SOP ATll- Exhibit AT11.2, revision 07-01-12 Page 42 of 99
1'"*_,* _.'* . lETS Page 3 of7 t~t*_.:*_. l 6 Environmental Testing Sclutlons.lnc. Species: Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant. Outfall 101 Date: 08*J\f-- I 1-CONC: 21.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced a 0 0 0 0 0 0 0 0 c Adult mortality \_..
'--- L '-- L. '-- L. \......
L. '- 2 Young produced 0 0 0 a 0 0 0 0 0 Q
\._ L Adult mortality
'- '- L \_.. L L. L 3 Young produced C) 0 b b 0 0 0 0 0 0 L L L_
Adult mortality ~\_ L. L..... \.._
'-- -q L '--
4 Young produced ~ $ s 5 "-' s 5 '-\ q Adult mortality
'- '- '--- \.._
'-- '-- \.... L.. L.. \..._
5 Young produced \1;:) \\ \"l- \0 \C \~ \'S \~ \\ '~ Adult mortality \........ \...... \.._ L.. \......
'- \...... \...._ \....... '-'
6 Young produced D 0 0 0 0 0 0 TI 0 0 7 Adult mortality Young produced a*& L
\S
'- L
\\o L..
\\..
\...._
\'\
\._
l-'\
\......
\\. ,.,
\......_ L-
\~
Total young produced Final Adult Mortality o\o. "!:.\ L
~0
\.......
'!\
ob ~'.,
- !.c; ~~
31- '!.0
\.......
'-- \...... '- \....,.
Note: Adult mortality (L =live, D =dead), SB - split brood (single brood split between two days), CO- can ovei7offsorimt carried over with adult durin2 transfer). Concentration:
% Mortality: 01.
Mean Offspring/Female: 32.R
%Reduction from Control- I:
- s. \7.
CONC: 43.2% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 (') 0 D 0 a D C) 0 0 Adult mortality
'- \..... L L L- L- L L- L L.
2 Young produced C) 0 D 0 0 0 C) 0 6 0 Adult mortality L L L \._ L L L L L L 3 Young produced aL ~ 0 0 0 6 .t'J 0 0 {) Adult mortality
'- '- L L L L L L. L.
4 Young produced \, s s ~ ~ -s l.o s '-1' s Adult mortality L \._.. \.... L. L L L L \.._ 5 Young produced
'~ \L \\ \\ IL.. 11..
'a"'
\1,.. l1.. -
\._ '-.. L L Adult mortality L L '- L L L..
6 Young produced 0 0 0 0 0 0 ~ a a 7 Adult mQrtality Young produced \&
'-- ,., L
\i
\
\~
\... l -
'~ \~
L... L
\t\
L
*s *~
L l9 I Total young produced
~, 1.'-\
3'\ 3\ 3\ !a\ L 31
\....
3'-l
~""
~s
\......
I Final Adult Mortality \..... \..._
'--- L \....
Note: Adult mortality (L =live, D =dead), SB- split brood (single brood split between two days), CO_, car overloffsorin2 carried over with adult durin2 transfer). Concentration:
%Mortality: 07.
~ Mean Offspring/Female: 33.1
% Reduction from Control-!: -a.~7.
I Page 43 of 99 SOP ATil- Exhibit AT11.2, revision 07-01-12
!ETS Page4 of7 0 EnvlronmentaiTesttngSolutlonr,lnc.
Species: Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant. OutfalllOl Date: _ _....;::0;....;:8=-*-=-l"l..,=."'__;I;..._1..-~-- CONC: 86.4% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced (J 0 0 0 0 0 0 D 0 0 Adult mortality L- \.... '- L.. L- L- L L- L L 2 Young produced D 0 a () () 0 0 0 CJ 0 Adult mortality
'-- '-- L L L '- L L L L-3 Young produced 0 0 D 0 0 D 0 c *o C) 4 Adult mortality Young produced s ,
\.... ~ '-- L 5
L
'-- L '--
s '- s \.. c. L L.. L_ 5 Adult mortality Young produced L-
\~
L.
\"'l-L.
\ "'L. \\
L...
\
\....... \._
11.. \~\ L
\L \~
\...... L
\?,
Adult mortality L \...... '- \........ '-- L '-- L... '- \...... 6 Young produced C\ 0 b D 0 0 e 6 0 () 7 Adult mortality Young produced
\'- \S \, '-
\~
'- \._
\
L-
\~
L...
\.4 L._
\~
'-- \....-
Total young produced
?l-\ ~~ !.\o ~~ !.5 3~ .3'\ ~~ ~lo 3.\
Fi.nal Adult Mortality L '- '-- \..... \..... L "'- '- '-- '--- Note: Adult mortality (L -live, D =dead), SB =split brood (single brood split between two days), CO= can over (offspring carried over with adult during transfer). Concentration:
%Mortality: ()7.
~.a Mean Offspring/Female:
%Reduction from Control-1: -n. S7.
CONC: 100% Survival and Reproduction Data Replicate number
- Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 l_.
0 0 D a c 0 0 0 Adult mortality L L L L L L- L. L- L 2 Young produced D 0 0 0 0 0 0 a 0 0 Adult mortality L- L.. L.. L.. \..._ L L \...... L. L.... 3 Young produced 0 a tJ 0 0 () a 0 () a 4 Adult mortality Young produced L
~
'-- L
.s L L.
5 L s s
'- L.
Co
\..._.
t, L
~
Adult mortality L l'-\ ,,_'-- L... L* L L L L L L l~
*~-
5 Young produced lb \""L \'-\ \'b \~ Adult mortality \.... \._ L L L \._ L.. L. L L 6 Young produced () 0 0 0 0 0 0 0 0 (). 7 Adult m~rtality Young produced
\&
\'
L L
'~
L 1(. L I~ ., ,, L. L L "2..0 1& L L_ 11 Total young produced 3ft .31 31 3'--l ~1.. 34 aS ~ 31 38 Final Adult Mortality '- \... \.._ \.... L. L L. L. \..___ *'--... Note: Adult mortality (L =live, D =dead), SB =split brood (single brood split between two days), CO= can over (offSpring carried over with adult during transfer). Concentration:
%Mortality: C7.
3(,.~ Mean Offspring/Female: I %Reduction from Control-1: -.1.07. I Page 44 of 99 SOP ATil- Exhibit AT11.2, revision 07-01-12
Page 5 of7 Species: Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant, OutfalllOl Date: __o_A_*_l_'-l_*_l'___ "L _ CONTROL-2 Survival and Reproduction Data Replicate number Day. 1 2 3 4 5 6 7 8 9 10 1 Young produced (") c () 0 0 a aL- 0 0 0 Adult mortality
'- L. L L. '-- L \..... L- L..
2 Young produced 0 0 0 0 0 0 0 0 (} 0 Adult mortality L \.....- L L. '-- '-- L. '-- ~ L_ 3 Young produced D D 0 D D a 0 0 0 a 4 Adult mortality Young produced
\..
'-\
- s'-- s \.._ \.._
~ \o
'L
'-(
\
4 L 4 L 4 L L
.'-\
'\ 5 Adult mortality Young produced Adult mortality L.
\0
\.....-
\'"Z...
0 L
\b a a
\.._
\0
\.......
\0
\.._.
L..
\C
\~
.c L
\\
0
\.......
L a
1 6 Young produced D 0 0 0 Adult mortality \.._.. \._
'-- \L\..\ \.._
'- \.- L '- L..
7 Young produced 1\. \i \\o \~ \S \~ \4 \U. \S Total young produced
"!>~ ~ ~"- ~
~
'~ ~"L :!:>\ '3\
"'S:)
Final Adult Mortality '- ~ '-- '- '-- \..... '- '- "- "-- X for 3rd Broods Note: Adult mortality (L
~ 'X- .
X
-live, D- dead), SB- spht brood (smgle brood split between two days), ~0-
~ ~ ~ ~ >""
carry over (offspnng earned over With adult dunng transfer) . X coNe: 100% Intake Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L. L L L L L- L L- L- L 2 Young produced 0 0 0 0 0 0 0 0 0 () Adult mortality
'-- '-- L L L- L- '--- \....... \._.. L.
3 Young produced {) 0 D D 0 0 0 0 0 0 4 Adult mortality Young produced L (., ~ L L-
\o s L..
s
\._
L L.. s L s L
\.._
'L L
4 ._ L. L Adult mortality \.... '-
,,__ '\...._ '-- L.. '--
5 Young produced Adult mortality
\\
'- ~
\'1..
\......
'~
lb L.
'"\
L..
\1...
L. t"l.. l~ L '\L... 6 Young produced 0 () a 0 0 0 a _Q L 0 [) Adult mortality L L \._ L L L L L L
\1::> \S \& \~ \~
7 Young produced Total young produced
\\o 3~ "1'\ 3~
\~
0~ 3~ 3to
\lo
'3~ 32 3fo'" \"'"\
3'\ Final Adult Mortality '- \..... '- '-- '-- '-- '-- \...... L. '-- Note: Adult mortality (L -live, D =dead), SB- split brood (single brood split between two days), CO= can over (offsprin2 carried over with adult durin2 transfer). Concentration:
%Mortality: 07.
Mean Offspring/Female: ~~--z.:
%Reduction from Control-2: -f.,. I 7!
SOP AT11- Exhibit AT11.2, revision 07-01-12 Page 45 of 99
TVA I Sequoyah Nuclear Plant, OutfalllOl- Non-treated August 14-21, 2012 Page 46 of 99 Verification of Ceriodaphnia Reproduction Totals Control-1 Re!llicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 5 5 5 5 6 4 6 49 4 5 7 5 5 4 5 6 5 6 6 54 5 11 11 10 12 10 9 12 11 11 11 108 5 13 12 12 11 12 12 14 12 13 13 124 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 14 18 15 16 16 14 17 15 15 15 155 7 16 15 19 18 19 16 14 18 17 18 170 Total 30 33 29 33 31 28 34 32 30 32 312 Total 34 34 36 34 35 33 34 35 36 37 348 10.8% 100% Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 6 4 4 6 6 4 6 5 5 51 4 6 7 5 6 5 5 5 6 6 6 57 5 13 12 11 12 12 10 13 12 12 12 119 5 14 12 13 12 12 14 13 13 13 13 129 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 18 16 18 17 17 19 17 16 14 15 167 7 18 18 19 16 15 19 17 20 18 19 179 Total 36 34 33 33 35 35 34 34 31 32 337 Total 38 37 37 34 32 38 35 39 37 38 365 Control-2 Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 5 5 6 5 5 4 4 4 48 4 4 5 5 5 6 4 4 4 4 4 45 5 13 11 12 10 10 13 13 13 11 13 119 5 10 12 13 12 10 10 10 13 11 12 113 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 18 15 16 16 14 19 17 16 17 13 161 7 16 17 16 14 16 15 18 14 14 15 155 Total 36 31 33 31 30 37 35 33 32 30 328 Total 30 34 34 31 32 29 32 31 29 31 313 43.2°/o 100% Intake Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 5 5 4 5 5 6 5 4 5 50 4 6 4 6 5 5 7 5 5 4 6 53 5 13 12 12 12 11 11 12 14 12 12 121 5 11 12 12 13 10 14 12 12 13 11 120 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 18 17 17 15 15 15 19 15 18 18 167 7 16 13 15 15 18 15 16 15 19 17 159 Total 37 34 34 31 31 31 37 34 34 35 338 Total 33 29 33 33 33 36 33 32 36 34 332 File: sqn101_081412data.xlsx 4---- Entered by: J. Sumner Reviewed by:
TVA I Sequoyah Nuclear Plant, Outfall101 Non-treated August 14-21, 2012 Page 47 of 99 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: _ _ _ _ _ _ _ _ _ _ _8_2_0_7 Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from variation (%) control (%) (%) (%) (offspring/female) 1 2 3 4 5 6 7 8 9 10 Control-1 30 33 29 33 31 28 34 32 30 32 100 31.2 6.2 Not applicable 10.8% 36 34 33 33 35 35 34 34 31 32 100 33.7 4.4 -8.0 21.6% 36 31 33 31 30 37 35 33 32 30 100 32.8 7.6 -5.1 43.2% 37 34 34 31 31 31 37 34 34 35 100 33.8 6.7 -8.3 86.4% 34 34 36 34 35 33 34 35 36 37 100 34.8 3.5 -11.5 100% 38 37 37 34 32 38 35 39 37 38 100 36.5 6.0 -17.0 Control- 2 30 34 34 31 32 29 32 31 29 31 100 31.3 5.6 Not applicable 100% Intake 33 29 33 33 33 36 33 32 36 34 100 33.2 6.0 -6.1 Outfall101: MSD = Minimum Significant Difference Dunnett's MSD value: 2.021 PMSD = Percent Minimum Significant Difference PMSD: 6.5 PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effiuent toxicity test. Intake: Dunnett's MSD value: 1.459 LowerPMSD bound determined byUSEPA (lOth percentile) = 13%. PMSD: 4.7 Upper PMSD bound determined by USEPA (90th percentile)= 47%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200la; USEPA, 2001b). USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effiuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200la, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effiuent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: sqn101_081412data.xlsx Table populated from associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheet. Spreadsheet entered by: J. ~ner Reviewed by:
TVA I Sequoyah Nuclear Plant, Outfall101 Non-treated August 14-21, 2012 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 8/14/2012 Test ID: CdFRCR Sample ID: TVA/SQN 101 End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Cone-% 1 2 3 4 5 6 7 8 9 10 Control-1 30.000 33.000 29.000 33.000 31.000 28.000 34.000 32.000 30.000 32.000 Control-2 30.000 34.000 34.000 31.000 32.000 29.000 32.000 31.000 29.000 31.000 10.8 36.000 34.000 33.000 33.000 35.000 35.000 34.000 34.000 31.000 32.000 21.6 36.000 31.000 33.000 31.000 30.000 37.000 35.000 33.000 32.000 30.000 43.2 37.000 34.000 34.000 31.000 31.000 31.000 37.000 34.000 34.000 35.000 86.4 34.000 34.000 36.000 34.000 35.000 33.000 34.000 35.000 36.000 37.000 100 38.000 37.000 37.000 34.000 32.000 38.000 35.000 39.000 37.000 38.000 Intake 33.000 29.000 33.000 33.000 33.000 36.000 33.000 32.000 36.000 34.000 Transform: Untransformed Rank 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean Control-1 31.200 0.9968 31.200 28.000 34.000 6.193 10 33.800 1.0000 Control-2 31.300 1.0000 31.300 29.000 34.000 5.645 10 10.8 33.700 1.0767 33.700 31.000 36.000 4.435 10 140.00 75.00 33.800 1.0000 21.6 32.800 1.0479 32.800 30.000 37.000 7.578 10 122.00 75.00 33.800 1.0000 43.2 33.800 1.0799 33.800 31.000 37.000 6.660 10 136.50 75.00 33.800 1.0000 86.4 34.800 1.1118 34.800 33.000 37.000 3.532 10 151.00 75.00 33.800 1.0000 100 36.500 1.1661 36.500 32.000 39.000 5.954 10 150.50 75.00 33.800 1.0000 Intake 33.200 1.0607 33.200 29.000 36.000 5.991 10 Auxilia~ Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.05) 0.98421 0.895 -0.0927 -0.4597 Bartlett's Test indicates equal variances (p = 0.35) 5.58769 15.0863 The control means are not sianificantl:z:: different{!;!= 0.91} 0.12078 2.10092 H~pothesls Test {1-tall, 0.05~ NOEC LOEC ChV TU Steel's Many-One Rank Test 100 >100 1 Treatments vs Control-1 Linear Interpolation (200 Resamples) Point % SD 95%CL Skew IC05 >100 IC10 >100 IC15 >100 1.0 IC20 >100 0.9 IC25 >100 0.8 IC40 >100 0.7 IC50 >100 0.6 Ql 0.5 Ill c 0.4 0 i 0.3 tt:: 0.2 0.1 0.0
-0.1
-0.2
-0.3 0 50 100 150 Dose%
Dose-Response Plot 45 40 35 0 30
~ 25
"'0
~ 20 Cll tt:: 15 10 5
0
~
gc: 1!c: CXl
~
lq N C'f (')
'<1:
co Cl) 8 8 File: sqnl01_081412dataxlsx Entered by: J. Srer Page 48 of 99 Reviewed by:~
TVA I Sequoyah Nuclear Plant, Outfall101 Non-treated August 14-21, 2012 Statistical Analyses Analysis used for PMSD calculation only. Cerioda~hnia Survival and Re~roduction Test-Re~roduction Start Date: 8/14/2012 Test ID: CdFRCR Sample ID: lVA/ SQN 101 End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Cone-% 1 2 3 4 5 6 7 8 9 10 Control-1 30.000 33.000 29.000 33.000 31.000 28.000 34.000 32.000 30.000 32.000 Control-2 30.000 34.000 34.000 31.000 32.000 29.000 32.000 31.000 29.000 31.000 10.8 36.000 34.000 33.000 33.000 35.000 35.000 34.000 34.000 31.000 32.000 21.6 36.000 31.000 33.000 31.000 30.000 37.000 35.000 33.000 32.000 30.000 43.2 37.000 34.000 34.000 31.000 31.000 31.000 37.000 34.000 34.000 35.000 86.4 34.000 34.000 36.000 34.000 35.000 33.000 34.000 35.000 36.000 37.000 100 38.000 37.000 37.000 34.000 32.000 38.000 35.000 39.000 37.000 38.000 Intake 33.000 29.000 33.000 33.000 33.000 36.000 33.000 32.000 36.000 34.000 Transform: Untransformed 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-1 31.200 0.9968 31.200 28.000 34.000 6.193 10 Control-2 31.300 1.0000 31.300 29.000 34.000 5.645 10 10.8 33.700 1.0767 33.700 31.000 36.000 4.435 10 -2.828 2.287 2.021 21.6 32.800 1.0479 32.800 30.000 37.000 7.578 10 -1.810 2.287 2.021 43.2 33.800 1.0799 33.800 31.000 37.000 6.660 10 -2.941 2.287 2.021 86.4 34.800 1.1118 34.800 33.000 37.000 3.532 10 -4.072 2.287 2.021 100 36.500 1.1661 36.500 32.000 39.000 5.954 10 -5.995 2.287 2.021 Intake 33.200 1.0607 33.200 29.000 36.000 5.991 10 Auxilia~ Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.05) 0.98421 0.895 -0.0927 -0.4597 Bartlett's Test indicates equal variances (p = 0.35) 5.58769 15.0863 The control means are not significant!}: different ~E = 0.91~ 0.12078 2.10092 H~othesis Test {1-tail, 0.05~ NOEC LOEC ChV TU MSDu MSD~ MSB MSE F-Prob df Dunnett's Test 100 >100 1 2.02145 0.06479 32.12 3.90741 7.9E-06 5,54 Treatments vs Control-1 File: sqn101_081412data.xlsx Entered by: J. Sumner Page 49 of 99 Reviewed b y : +
TVA I Sequoyah Nuclear Plant, Intake Non-treated August 14-21, 2012 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 8/14/2012 Test ID: CdFRCR Sample ID: TVA I SQN 101 -Intake End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Cone-% 1 2 3 4 5 6 7 8 9 10 Control-1 30.000 33.000 29.000 33.000 31.000 28.000 34.000 32.000 30.000 32.000 Control-2 30.000 34.000 34.000 31.000 32.000 29.000 32.000 31.000 29.000 31.000 10.8 36.000 34.000 33.000 33.000 35.000 35.000 34.000 34.000 31.000 32.000 21.6 36.000 31.000 33.000 31.000 30.000 37.000 35.000 33.000 32.000 30.000 43.2 37.000 34.000 34.000 31.000 31.000 31.000 37.000 34.000 34.000 35.000 86.4 34.000 34.000 36.000 34.000 35.000 33.000 34.000 35.000 36.000 37.000 100 38.000 37.000 37.000 34.000 32.000 38.000 35.000 39.000 37.000 38.000 Intake 33.000 29.000 33.000 33.000 33.000 36.000 33.000 32.000 36.000 34.000 Transform: Untransformed 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-1 31.200 0.9968 31.200 28.000 34.000 6.193 10 Control-2 31.300 1.0000 31.300 29.000 34.000 5.645 10 10.8 33.700 1.0767 33.700 31.000 36.000 4.435 10 21.6 32.800 1.0479 32.800 30.000 37.000 7.578 10 43.2 33.800 1.0799 33.800 31.000 37.000 6.660 10 86.4 34.800 1.1118 34.800 33.000 37.000 3.532 10 100 36.500 1.1661 36.500 32.000 39.000 5.954 10 Intake 33.200 1.0607 33.200 29.000 36.000 5.991 10 -2.258 1. 734 1.459 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.05) 0.91798 0.905 -0.1715 0.33113
=
F-Test indicates equal variances (p 0.73) 1.2669 6.54109 The control means are not significantly different (p = 0.91) 0.12078 2.10092 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.45886 0.04661 18.05 3.53889 0.03657 1, 18 Treatments vs Control-2 Dose-Response Plot 45 40 35 1-tail, 0.05 level 0 30 of significance
+I g 25 "0
e 20 Q. Q) 0:: 15 10 5 0 ......
~ <X)
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- g :g I
0
...... c;:j ("I) co
'V <X) 1:: 1::
8 8 File: sqnl 01_081412data.xlsx Entered by: J. Swnner Page 50 of 99 Reviewed by:+-
Page 6 of7 Species: Ceriodaphnia dubia Date: _-3o&...::i:::.-*--='......!'-l._*.:_l,_._.:::::.__ _ Client: TVA I Seguoyah Nuclear Plant. OutfalllOl CONTROL 10.8% 21.6% 43.2% 86.4% 100% I I I 100% Intake
*Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench
- sheet specific for each analysis and transcribed to this bench sheet by: ~
SOP AT11- Exhibit AT11.2, revision 07-01-12 Page 51 of 99
!ET *
- EnvlronmentalTestingSolutlons.lnc.
Page 7 of7 Species: Client: Date: OC* Concen- Parameter tration CONTROL 10.8% 21.6% 43.2% 86.4% 100%.
).
100% Intake
*Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: ~
SOP ATil- Exhibit AT11.2, revision 07-01-12 Page 52 of 99
TVA I Sequoyah Nuclear Plant, Outfall101 -Non-treated August 14-21, 2012 Page 53 of 99 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Project number: _ _ _ _ _ _82_0_7 Control 10.8% 21.6% 43.2% 86.4% 100% 100% Intake File: sqn101_081412chem.xls Entered by: J. Sumner Reviewed by: Q{
Page 1 of6
~r'i 11-, -
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: Tennessee Valley Authority County: Rhea Facility: Seguoyah Nuclear Plant Outfall: 101 NPDES #: TN0020168 Project #: --=':......;1.=-tr\=......a..._ _ _ __ I Dilution preparation information: Dilution prep(%) 10.8 21.6 43.2 86.4 100 Comments: Each concentration was UV-treated Effluent volume (mL) 270 540 1080 2160 2500 for 2 minutes to remove pathogenic I Diluent volume (rnL) Total volume (mL) 2230 2500 1960 2500 1420 2500 340 2500 0 2500 Interferences. I! Test organism information: Test information: Organism age: ""'lt)
- 1.1.. Uftal Jt C:.. Q l.b Randomizing template: ~
Date and times organisms Ol *\~* \1... \\o()() Incubator number and
?lf>
I were born between: Organism source: ATb"" '&A~t\"\ ~ ot*a?t*tl.. shelf location: Artemia CHM number: t.l'Kt.S1-Drying information for weight l"'i Transfer bowl information: pH= i.\oO s.u. determination: Date I Time in oven: ol*"L\* \1.. Temperature = 1..S.\ oc Initial oven temperature: \o() *t.- Average transfer volume: Date I Time out of oven: Ol*"tLa \"'J. 0.\1.0~ Final oven temperature: ~*~ Total drying time: '"lA*\-\~ Daily feeding and renewal information: r Day Date
- Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch I termination used 0
2 3 4 5 I 6 7 I I lndependen0 Review by SOP AT20- Exhibit AT20.3, revision 07-01-12 Kelley E. Keenan I~ Page 54 of 99
Page 2 of6 Species: Pimephales promelas Client: TVA I Sequoyah Nuclear Plant, OutfalllOl, UV-treated Survival and Growth Data Day L:U.NT.KOL 10.8% 21.6% A B c D E F G H I J K L 0 10 10 /{) It> 10 /D /D ID /() /C /() IO 1 ro 10 10 10 10 10 10 10 /0 /0 /0 10 2 10 10 10 /0 tO 10 /0 10 /Q 10 10 10 3 {f;J I() /Q l.O 10 10 /0 IO IO 10 10 10 4
/0 ID /0 /0 10 /0 /0 /0 IO /Q 10 10 5
'.0 10 /Q' /() /Q 10 10 IO /0 ID /0 IQ 10 10 to tO 10 IO /0 6
10 IO ro '0 10 7 1c~.._ ll) I
/0 ((J /0 I(J 10 ID (() tO 10 10 A= Pan weight (me Tray color code:: ;E: 6lu, t4.4b tS.*1 t4.te,z 14.z.c. lt.B4 t4.!S Analyst: M IZ.SS 14.~-s 13.33 13.1'\ 14.0G, 13.tt Date: nB*QO*tt.
B =Pan + Larvae weight (mg) Analyst: Date:
~.
~*~"'7-l'Z.
17.'1 '8 l'f.Z S -t.l-3' %."*'~ [1.0. 0' 2.0. '11 z.as7 \4.1'8 ZD:!£1 I'S-"13 1'1.53 1$-13 II C =Larvae weight (mg) =B- A Hand calculated. S.\0 "\.lt s.&1 <<6.\~ c...1~ s.s~ eo.~\ IS.'\"\ S.'Y\ $ .. 1.~ s."\1 ~. &"L Analyst: . J~ I Weight per initial number of larvae (mg) r t.;~
= C I Initial number of larvae t.;~ c,~
~ " ~ ~ "':>... \,'V
~ ~
o*" 1.;; Hand calculated. I Analyst: 4~
&"i r::l ~*
~
o* "' 1().
\0 C>. o* o*
\D o* b. f:).
o* . Average weight per Percent reduction initial number of from control (~) o.~~s 0. \:a\'-\ - ,....4\ 7. ().~\,~ -~:~1. I larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sin =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. I Comments: I I I Page 55 of 99 SOP AT20- Exhibit AT20.3, revision 07-01-12
TS Page 3 of6 Species: Pimephales promelas Client: TVA I Sequoyah Nuclear Plant, OutfalllOl, UV-treated Date: Survival and Growth Data Day 43.2°/o 86.4o/o 100%
; M N 0 p Q R s T u v w X 0
ro ro to lC) ID lQ IO /C) 10 tO 10 10 1 10 10 10 10 10 tO /0 lD /{) 10 IQ 'O 2 I (0 (0 10 /0 I() 10 tO /C 10 10 10 /D 3 IO lQ 10 10 to /0 10 10 10 10 10 10 4 JD 10 10 10 ID 10 I() /Q 10 10 10 10 5 10 10 IO 10 IO IO 10 q*tA /0 IO IO IO 6
/0 /0 IC /0 /D IO 10 '1 JC /Q 10 /0 lP\
I 7 (() fO 10 /0 /() It) ID '1 I~ ID lb 1(3*' A= Pan weight (mg) Tray color code:: ~ Sl~ Analyst: t4.oB ,3,41 t~.<<b'3 13.0 13.2.1 *3.Gf\ -~3.~G, t.f.sz. 14.33 1~.~'3 13.z.4. IZ.tC. Date: a8*aL.*rz. B =Pan + Larvae weight (mg) Analyst: ;:s:'Ul ,q ..5C:J tq,"a5 17.70 7..t>.oo \8.'3 l'f.~l ICJ.o5 tS.3' '2J.1G 14."17 ltl'g *~50 Date: O~*'Z7*1Z. C = Larvae weight (mg) = B- A s .4\1. ,... ~.1Jo t.\.41\'-l Hand calculated. Analyst: $1~ Weight per initial number of larvae (mg) S.l'- ~.qt s.tt s.ot\ '$.1."'\ !..<<:~\
"*" s.~ ~
= C I Initial number of larvae '
~ ,_,~ t' ,..,~ ~'Jo ,_,o ~ ,., ~
o*"~
,..,\/}
Hand calculated. if' o*,t t;; ~ ~ ~ Analyst: Average weight per el~ Percent reduction o' o* o* (:;) o* f;;;). '?
~* o* o* o*
initial number of from control (%) o.~\o\ -~ .() 1, o .soC\ c.t *.&T. o.~~'\ o. \ i~ I larvae(mJd Comment codes: c = clear, d = dead, fg = fungus, k =killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. I Comments: I I I Page 56 of 99 SOP AT20- Exhibit AT20.3, revision 07-01-12
TS Page 4 of6 Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant, OutfalllOl, UV-treated Date: 0 &* a'=\* I,__ Day 0 10 1 10 2
/0 10 3
ID (() /0 4 10 10 10 5
/Q IO 10 6
10 tO /0 7 IC /() 10 A= Pan weight (mg} Tray color code:: L~+ Slw.. Analyst: _ __.::i~~~---- IS.2.2, 14.lot Date: oA.ot. *\1.. B =Pan + Larvae weight (mg) Analyst: :n..& 9'f tcr. z.s 1."13 to.3'f Date: 09*2.7.&2. C = Larvae weight (mg) = B- A Hand calculated. Analyst: A
~ -
Weight per initial number oflarvae (mg) Ct. "1..\ 5 *.,~ ~.'41
= C I Initial number of larvae Hand calculated.
Analyst: _ ___,.~----- Average weight per Percent reduction initial number of from control(%) o.s~\ I larvae Comment codes: c = clear, d = dead, fg = fungus, k =killed, m =missing, sk = sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. I Comments: I I I Page 57 of 99 SOP AT20 - Exhibit AT20.3, revision 07-01-12
TVA I Sequoyah Nuclear Plant, Outfall101 UV-treated Page 58 of 99 August 14-21, 2012
** Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc. Project number: 8207 Not for Compliance Assessment, Internal Laboratory QC Concentration(%) Replicate Initial number of Final number of larvae A- Pan weight (mg) B- Pan+ Larvae Larvae weight (mg) Weight I Surviving Mean weight I Coefficient of variation Weight I Initial number Mean survival Mean weight I Initial Coefficient of Percent reduction from larvae weight(mg) =A-B number oflarvae (mg) Surviving number of (Menweightpersuniving of larvae (mg) (%) number oflarvae variation (Mean weight control(%)
larvae (mg) numberorlarvae)(%) (mg) perinitialnumberoflanrae) (%) A 10 10 12.88 17.98 5.10 0.510 0.510 B 10 10 14.40 19.28 4.88 0.488 0.488 Control 0.535 8.3 100.0 0.535 8.3 Not applicable c 10 10 15.49 21.36 5.87 0.587 0.587 D 10 10 14.63 20.16 5.53 0.553 0.553 E 10 10 13.33 20.06 6.73 0.673 0.673 F 10 10 14.62 20.21 5.59 0.559 0.559 10.8% 0.614 8.0 100.0 0.614 8.0 -14.9 G 10 10 14.26 20.57 6.31 0.631 0.631 H 10 10 12.84 18.78 5.94 0.594 0.594 I 10 10 14.35 20.34 5.99 0.599 0.599 J 10 10 13.19 18.43 5.24 0.524 0.524 21.6% 0.563 6.0 100.0 0.563 6.0 -5.3 K 10 10 14.06 19.53 5.47 0.547 0.547 L 10 10 13.11 18.93 5.82 0.582 0.582 M 10 10 14.08 20.00 5.92 0.592 0.592 N 10 10 13.47 18.63 5.16 0.516 0.516 43.2% 0.561 6.4 100.0 0.561 6.4 -5.0 0 10 10 13.53 19.01 5.48 0.548 0.548 p 10 10 13.17 19.05 5.88 0.588 0.588 Q 10 10 13.27 18.36 5.09 0.509 0.509 R 10 10 13.69 18.96 5.27 0.527 0.527 86.4% 0.521 4.5 97.5 0.509 8.6 4.8 s 10 10 13.96 19.47 5.51 0.551 0.551 T 10 9 14.82 19.30 4.48 0.498 0.448 u 10 10 14.33 19.83 5.50 0.550 0.550 100% v 10 10 12.73 18.38 5.65 0.565 0.534 5.8 0.565 100.0 0.534 5.8 0.1 w 10 10 13.24 18.50 5.26 0.526 0.526 X 10 10 12.76 17.70 4.94 0.494 0.494 y 10 10 15.22 21.43 6.21 0.621 0.621 100% Intake z 10 10 14.61 20.34 5.73 0.573 0.581 12.5 0.573 100.0 0.581 12.5 -8.7 AA 10 10 14.37 20.84 6.47 0.647 0.647 BB 10 10 14.39 19.21 4.82 0.482 0.482 Outfall 101: MSD= Minimum Significant Difference Dunnett's MSD value: 0.0685 PMSD= Percent Minimum Significant Difference PMSD: 12.8 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Intake: Dunnett's MSD value: 0.0826 Lower PMSD bound determined by USEPA (1Oth percentile) = 12%. PMSD: 15.5 Upper PMSD bound determined by USEPA (90th percentile) = 30%. Lower and upper PMSD bounds were determined from the I Oth and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001 b). USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effiuent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: sqn101_081412data-uv.xlsx Entered by: J. Sumner Reviewed by: Jf--
TVA I Sequoyah Nuclear Plant, Outfall101 UV-treated August 14-21, 2012
*6* Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 8/14/2012 Test ID: PpFRCR Sample ID: TVA I SQN 101 End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Cone-% 1 2 3 4 D-Control 0.5100 0.4880 0.5870 0.5530 10.8 0.6730 0.5590 0.6310 0.5940 21.6 0.5990 0.5240 0.5470 0.5820 43.2 0.5920 0.5160 0.5480 0.5880 86.4 0.5090 0.5270 0.5510 0.4480 100 0.5500 0.5650 0.5260 0.4940 Intake 0.6210 0.5730 0.6470 0.4820 Transform: Untransformed 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5345 1.0000 0.5345 0.4880 0.5870 8.269 4 0.5744 1.0000 10.8 0.6143 1.1492 0.6143 0.5590 0.6730 7.973 4 -2.807 2.410 0.0685 0.5744 1.0000 21.6 0.5630 1.0533 0.5630 0.5240 0.5990 6.009 4 -1.003 2.410 0.0685 '0.5630 0.9802 43.2 0.5610 1.0496 0.5610 0.5160 0.5920 6.414 4 -0.933 2.410 0.0685 0.5610 0.9767 86.4 0.5088 0.9518 0.5088 0.4480 0.5510 8.649 4 0.906 2.410 0.0685 0.5213 0.9075 100 0.5338 0.9986 0.5338 0.4940 0.5650 5.806 4 0.026 2.410 0.0685 0.5213 0.9075 Intake 0.5808 1.0865 0.5808 0.4820 0.6470 12.504 4 Auxilia!l Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94963 0.884 -0.1675 -1.1562 Bartlett's Test indicates egual variances {E = 0.97l 0.84084 15.0863 H~~othesis Test {1-tail, 0.05l NOEC LOEC ChV TU MSDu MSD~ MSB MSE F-Prob df Dunnett's Test 100 >100 0.06848 0.12811 0.00527 0.00161 0.02854 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 95% CL(Ex~l Skew IC05 59.875 IC10 >100 IC15 >100 1.0 IC20 >100 0.9 IC25 >100 0.8 IC40 >100 0.7 IC50 >100 0.6 Q) Ill 0.5 c 0 0.4 m- 0.3 0:: 0.2 0.1 0.0
-0.1 *,'
-0.2
-0.3 0 50 100 150 Dose%
Dose-Response Plot 0.8 0.7 0.6 J 0.5 1-tail, 0.05 level
~ of significance
" 0.4
~
c..... 0.3 0.2 0.1 0
- g ()()
0
'<I:
CD 0 0 1: 0 co 0 0 File: sqn101_081412data-uv.xlsx
----w Entered by: J. Su~
Reviewed by: Page 59 of 99
TVA I Sequoyah Nuclear Plant, Intake UV-treated August 14-21, 2012 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 8/14/2012 Test ID: PpFRCR Sample ID: TVA I SQN 101 - Intake End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Cone-% 1 2 3 4 D-Control 0.5100 0.4880 0.5870 0.5530 10.8 0.6730 0.5590 0.6310 0.5940 21.6 0.5990 0.5240 0.5470 0.5820 43.2 0.5920 0.5160 0.5480 0.5880 86.4 0.5090 0.5270 0.5510 0.4480 100 0.5500 0.5650 0.5260 0.4940 Intake 0.6210 0.5730 0.6470 0.4820 Transform: Untransformed 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.5345 1.0000 0.5345 0.4880 0.5870 8.269 4 10.8 0.6143 1.1492 0.6143 0.5590 0.6730 7.973 4 21.6 0.5630 1.0533 0.5630 0.5240 0.5990 6.009 4 43.2 0.5610 1.0496 0.5610 0.5160 0.5920 6.414 4 86.4 0.5088 0.9518 0.5088 0.4480 0.5510 8.649 4 100 0.5338 0.9986 0.5338 0.4940 0.5650 5.806 4 Intake 0.5808 1.0865 0.5808 0.4820 0.6470 12.504 4 -1.088 1.943 0.0826 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95559 0.749 -0.6317 -0.2636 F-Test indicates egual variances (p = 0.44) 2.69933 47.4683 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.0826 0.15453 0.00428 0.00361 0.31833 1, 6 Treatments vs D-Control Dose-Response Plot 0.8 0.7 0.6 te 0.5 1-tail, 0.05 level C) 0.4 of significance
~
c...... 0.3 0.2 0.1 0
- g <X)
CC! c: 0
....c::i N
(.) 0 File: sqn 101_081412data-uv.xlsx Entered by: J. Sumner Reviewed by:~ Page 60 of 99
!ETS 6~ruiTttdngSoi11Uon .. lnc. Page 5 of6 Species: Pimephales promelas Date: ___0--=&;_*_1~_;;*;__1'-~--
Client: TVA I Seguoyah Nuclear Plant. OutfalllOl. UV-treated CONTROL UV-treated 10.8% 21.6% 43.2%" 86.4%. 100% 100% Intake
*Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table Alkal~nity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed b : ' ~ . Total residual chlorine was performed on non-treated Outfall! 01 and Intake samples.
v. Page 61 of 99
Page 6 of6
.t Concen- Parameter tration CONTROL ~~~~----~~~L_
UV-treated 10.8% 21.6% 43.2% 86.4% 100% 100% Intake
*Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet
,deftt-.:>,-"M,-** Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.
SOP AT20- Exhibit AT20.3, revision 07-01-12 Page 62 of 99
- ------------~---*-**-------~---.' -------------------------------------------------"----- --------
TVA I Sequoyah Nuclear Plant, Outfall101- UV-treated August 14-21, 2012 Page 63 of 99 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number: _ _ _ _ _ _8_2_07_ Concentration Control 10.8% 21.6% 43.2% 86.4% 100% 100% Intake File: sqn101_081412chem-UV.xls Entered by: J. Sumner Reviewed by:+
Page _7..:...:6=---
*=.E*,***r.*.* Page -+--_of 3 6 Environmental Testing Solutions, Inc.
Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Analyst I ;:r~ Time initiated I t5o3 Titrate samples to Date analyzed ==o'3=*=i=*=,'t.======= pH= 4.5 S.U. Time completed ===\='Ll='f========== Titrant normality and multiplier determination: pH of Normality Normality (N) of H 2S04 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na2C03 x 0.05)/E = (N x 50000)1100 ml sample water reference standard ml ml ml =0.25/E =Nx500
=4.5 s.u. number number (E) (acceptable range= 0.0180 - 0.0220)
SJ ~~""' :Z:NSS leW&' 0.0 \'t.' \'t*\ o.o-z.o7 \0.3 1!1\k cotr. o.o- c>.o.o.o L aboratory controI stan dar,d: Reference standard True value Sample Alkalinity (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO:YL) (acceptable range (mg CaCO:YL) (ml) ml ml ml =90 to 110%) XN.S:S 1o3/ 100 100 q.c l'l.. \ 1:\."7 10.3 '\'\.0% S~mple Alkalinity %RPD= volume Begin End Total Multiplier (mg CaCO:YL) {(S- D) /[(S+D)/2]} X 100 ml ml ml (acceptable range=+/- 10%)
,,0 s
'1.-'*7 t.7. ., tO..l 't
.11 Duplicate (B) D "2."7.7 "!.'3*..., ,,0 '1. -S-" Otjt*~*l't atnx spz e recovery: ...
Reference standard Spike value Sample Spike alkalinity (A) ~* number (SV) volume Begin End Total Multipl~er (mg CaCO:YL)
.. (mg CaCO:YL) (ml) ml ml ml
-::r.:Ns.s 1037 *so lCb z'/.7 31$.5 ,o.s (().3 l IO Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCO:YL) MV=A-B (acceptable range (mg CaCO:YL) =75 to 125%)
62 Lf'S I
~,.oY.
amp4 e measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml ' Multiplier (mg CaCO:YL) oe.,~.(L, IY\\t~w lQ:) 38.5 'i4.S ,,0 10.3
'1..
00 .\1. *.VI,.- l o.o ,,() ,,0
'2.
0~*\~. l'L- ~ ~~ 6.() \7.'3 \\.'3 \2.0
~ . ,o*tt. r!l J 17.3 "t.C0.3 \\.0 \10
()6.\'l*~ ,If \(() lAJu~ "' 6"0 Z.1.3 3~.2. 31.t. L(S.?
\O.'J
,,s ('Z.) l"30 0 t. \"'2. .. \~ ml-K. vv 1 '():;) c>.o ~0 '*G 'z a& .,'-\ ...,'1...
O:t*l\G*\-z.._
\
.lJ 3 2.
'II b.O l\.'1 1\.'\
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S.'f s .. ., ~v Reviewed by: Date reviewed: I oe, l q **"Z-Page 64 of 99 SOP C6 - Exhibit C6.1. revision 06-01-11
.!.E.T :
Page -z... Page If of-'3=---- 6 Environmental Testing Solutions, inc. Alkalinity (SM 2320 B) 1'~.-*,*.*,.* Analyst I ~~ Date analyzed 1--o-g-.-ti-~*-rt..----1 Matrix: Wat~r, RL = 1.0 mg CaC03/L Titrate samples to Time initiated Tim~ completed p :::;~,~ I pH= 4.5 S.U. Titrant normality and multiplier determination: pH of Normality Normality (N) of H 2S04 pH Factor or Multiplier J)eiOmzeu ......
.o.au-~.
,.h,.,.lr Beein End Total = (5 ml Na2C03 x 0.05)/E = (N x 50000)/100 ml sample water reference standard ml m* --ml- r-:--_ = 0.25/E =Nx500 4.5S.U. number number (E) (acceptaun~ * ..,. n n1 flln - 0.0220)
~0,*\~,'l L aboratory controI stan aard:
Reference standard True value Sample Alkalinity (MV) % RS=MV /TVx 100 number (TV) volume Begin End Total Multiplier (mgCaCOYL) (acceptable range (mg CaC031L) (ml} ml ml ml =90 to 110%)
- r~s 1031 100 100 17.9 %.1. '3 q.S" \a.3 q9 c:t~.o%
Alkalinity %RPD= Begin End Total Multiplier (mg CaCOliL) {(S- D) /[(S+D)/2]} X 100 ml ml ml (acceptable range = +/- 10%)
'2. ..,, 3 Jo,, s 72.
'3.5 Duplicate (B) D
~ 36-i '3'4.3 ~.5 7Z atrrx spz"ke recovery: **-
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaC031L) (m2 CaC031L) (ml) ml ml ml
*:t~s s 1037 \00 '5D 30.8 ~1.0 '5.l ~)\0.3 l7c Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (mgCaCOYL) MV=A-B (acceptable range (mgCaCOYL) =75 to 125%)
7Z *ct~o% Sample measurements: "" ' Sample volume Begin End Total Alkalinity r Sample number
\1.0 k\~.,0 Sample ill (ml) ml ml ml Multiplier (mg CaCOliL)
-lfJ
. .A. _c.Q.J *O a 2. fP 3'1.0 LIZ.. 'f 3.'-i (Z.) \0.3 7o I 17.01\1*01
\1.01 ,~.en.. IvA~ hJi
-11 .3 "\2..'-l o.o LfS.'t 3.3 l.S
'3.3 7'l.
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J a t.oB 11.
- z. oII~
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'Z.<>.Z.
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2.1." 3,4 l.Lf I~
,, 70 7o Reviewed by: Datereviewed: IOS*lq*l1..
Page 65 of 99 SOP C6 -Exhibit C6.1. revision 06-01-11
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Environmental Testing Solutions, Inc. Page 3 Page --=-7l=-_,__ of-=.3__ Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Analyst I 3'"\J Date analyzed 1--o--s.....;;.,-,-.-z----1 Time initiated ~~ Time completed t----=...._""'"""'~-.JQ:J---1 1 Titrate samples to pH= 4.5 S.U. 0'1*\t*lt Titrant normality_ and multiplier determination: pH of Normality Normality (N) of H 2S04 pH Factor or Multiplier
~ _._ ~. ......
...... check Begin End Total = (5 ml Na2C03 x 0.05)/E JJeiOIII.L~U = (Nx 50000)/ 100 ml sample water reference ~ r--mL. r--- f-...cEl ml ml =0.25/E =Nx500
=4~5 s.u. number number (acceptable range = 0.0180 - 0.0220)
-u:.P C>T*l I'* tz.
Lb a oratory controI stantlar,d.: Reference standard True value Sample Alkalinity (MV) %RS =MV /TVx 100 number (TV) volume Begin End Total Multiplier (mg CaCO:VL) (acceptabie range (mg CaCO:VL) (ml) ml ml ml =90 to 110%) 100 ;
""J:NSS \03'7 100 "Z.3.C' '33.\ C\.S to.3 Cf'S ~11.0%
. Alkalinity %RPD=
End Total Multiplier (mg CaCO:VL) {(S- D) /[(S+D)/2]} X 100 ml (acceptable range=+/- 10%) s ID D td) 3G.S 3'1.'1 70 ==G"'~ Of*l8*tt. M.atrtx
- sp1'ke recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mgCaCOYL) (m_g_ CaCO:VL) (ml) ml ml ml
- i:)\SS \03"7 \00 ~ '3,,5 Ll"'.7 4.l. ~\ \0.3 l?o Sample alkalinity (B) Measured spike value (MV) % R = MV I sv X 100 (mg CaCO:VL) . MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%)
7o IOO 10o.o% amp4 e measurements: .
~ Sample volume Begin End Total Alkalinity Sample numper At'~ Sample ID (ml) ml ml ml Multiplier (m2 CaCOJI.Ll \~0&11~* 'f'-'AS&~&mU~ 3 P Lf&f. 7 4~., '3.4 C:Z..1 \0.3 76 J ,~ ol tt..\ .. a~ ~d 100 d,, Lf .(
1.0 0."1
\'!. () &l\o .o \ J 2.. I \.0 4.'-t 5.'4 35 t
- 11. otli.- o \ ~k ..3 '1.'"{ 3,5 \l~ 3C 7.'
. -~
~ --------- ~ O"f*\'i*rl.
Reviewed by: Date reviewed: IDe*l q,ft., Page 66 of 99 SOP C6 - Exhibit C6.1. revision 06-01-11
Total Hardness (SM 2340 C) RL = 1.0 mg CaC03/L Analyst I ~ Date analyzed *:=oq=*=,~=*=ll.======= Titrant normality and multiplier determination: _,,,_,- :,r __ ,ic _:,:;.'-. Titrant Normality check Begin End Total Normality (N) of EDTA or pH Factor Mtlltipli~r reference standard ml ml ml =0.2/E = (Nx soooo)t50mlsafuple number number (E) (acceptable range = 0.0180 - 0.0220) =Nxtooo _ INI:. 1.(~'8 ":J:NSS to:sJ o.o IO.'t \O."L 0.0\'l' \Cf.C -* L aboratory control standard: Reference standard True value Sample Hardness (MV) % RS = MV I TV x100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaC03/L) (ml) ml ml ml =90 to 110%)
- Z:J.l.SS l 0 4 i' 40 50 IQ.'Z.. 1'2. "Z 'Z..O ,q,, 'act Cf7.5%
Sample Hardness %RPD= Sample Sample ID volume Begin End Total Multiplier (mg CaC03/L) {(S - D) /[(S+D)/2]} X 100 number ml ml ml
- 6) (~.7 \<t.6 s .g~
\'l.:Z. 4.5 D
S) ,,_ '1 1. \. t '-(.5 '815 atnx spz e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaC03/L) (ml) ml ml ml
- CNS.S \OLff 40 ~ *7 z.3."Z ,.5 \Cf.C \'30 Sample hardness (B) Measured spike value (MV) % R=MV /SVx 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaCOJIL) =75 to 125%)
'f>~ 42.. \oS.o%
ampJ e measurements: Sample volume Begin End Total Hardness SaQJ.ple number SampleiD (ml) ml ml ml Multiplier (mg CaC03/L) TV=ND Blank (should be = 0 mg CaC03/L) 60 O.c> 0.0 o.o Icr.C ND (.)e,) 11 .. 1~ IY\W5W 2.3.'2- 1..7.-, t.t. 5 I 'a<a' oe. ,.,_rt... Jt ~?.7 '3'2.1.( '1.7 'f2 SiJ~W.U.- (>a' 1\A- 32.4 33.$ I. l zz 04 .. \'Z.* 1; In t*S Lh.J .n.s .... ~<f 37.S' ~ oa *a'-'\.. ',_ 2.. 37.'i L(l.1 LI.J ~'1 OJ*) 'o_*l 'l.t 'Jt 3 L(Z..I "f6.&.f Lf-3 ~If
\'"'2..08\~()\ '1'J 1\ 5&~ 10\ \ 0,0 3.7 '3.1 -rz.
l'I.Ot\S. lO l z. 3.?' '7.3 '3h' 7l rz. oa a.,. 08- ~ 3 ~~ 7.!, tt.\ "'3.'ir ~~ 7£{ Page Note: 67 of If >15ml 99 is used, sample must be diluted. of titrant Reviewed by: Date reviewed I oe*~tq.ft.... n"~ ~,., ,..... .,. ..... -- . .
(t Page 3'7
~ *@J Page 7 of 3 Analyst I :r-~
Date analyzed ==og=*=\1=*=\'1======= Titrant normality and multiplier determination:
, '!!!!_ant Normality check Begin End Total Normality (N) of EDT A pH Factor or Multiplier reference -* -*-
., ...... ucuu 1111
.. = 0.21E = (N x 50000)1 50 ml sample number number (E) (acceptaun:: *-**e.~ n OHIO- 0.0220) Nx 1000
--~~ o~~l4£*l'l L aboratory controI stan dar,d:
Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaC03/L) (ml) ml ml ml =90 to 110%)
~NSS lOLl~ 40 50 II.\ (3.0
,_, l<t.C '37 1'Z..S?;
Hardness %RPD= Begin End Total Multiplier (mg CaC03/L) {(S- D) /[(S+D)/2]} X 100 ml ml ml
\3.0 s 72.
Duplicate (B)
~ IC.7
'* D
- 72. -.:r~o'\5-1~*\Z atrlX spz e recovery: ..
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mg CaC03/L) (ml) ml ml ml
- tJJ5S 10"{~ Lfo 8J \C. "7 'l'Z..'t 5."1 t1.C \\0 Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaC03/L) =75 to 125%)
71.. 3~ ' "{S.o% samp4l e measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC03/L) Hl..,.nlr T\r l'ILJ ~c::>s*t1'*(2:,. (should be= 0 mg CaC03/L) '-z.oi\S- \\ 1\/A ~~ 'Nl' 'L 6' l..'l..Lf 2&:Z '3.t (~., l'f \7J>& \1 .. 0'\ J, .3 '1.,, 'Z. 1..'\,q '3:7 7Z-
\ .OZ....o.i ' !. "() \ Tv AS&a..lao,uu ' ~cc.q 33., 3.7 7Z \"Ot\~.10 j 2.. '3'3., 37.'!1 '3.7 71. \"l.o&\,.0~ ~ 3 37.3 Lfi.O 3.7 n I "'1. OJr\ ~ * (J1.. TJ A Sl~tNTuu lfl.o 4'f.C 3.-G 71 \ 1..0& \~* l\ z..' 4"t., Lt1.3 3."7 -rz. \ '"2. 0' , _ d\ ~v 3 o.o '3.1 '3.7 7'Z. \~a& 1t.l. 0\ ~c)' \1~ 3:7 S.1 't.. \ ~LI Lfl Note: If >15ml oftitrant is used, sample must be diluted.
Page 68 of 99 Reviewed by: *I p}(,~ Date reviewed I re 'q I,_ SOP C7 - Exhibit C7 .1. revision 06-01-11
~
Page ({o
;l':\1 Page 3
___::;___ of 3
'3> ~ Environmental Testing Solutions, Inc.
Total Hardness (SM 2340 C) RL = 1.0 mg CaCOiL Analyst ~___;;;S;....;;03::;,c___ _ _-t Time initiated 1-::-:---..... Date analyzed o <6'. t1* \ -t. Time completed <:::::J\B
~t'*IS*~
Titrant normality and multiplier determination:
, Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier referen~~
u*-**~-* ~
-" ..... ml ml 0.2/E (N x 50000)/ 50 ml sample number number (E) (acceptable range u.vA~~ nO??m Nx 1000
--:--.-~~-&*\t* fl.
Laboratory controI stan dard: . Reference standard True value Sample Hardness (MV) % RS = MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJIL) (ml) ml ml ml =90 to 110%) XNSS toL{ 'IS 40 50 5".'8' 7.7 v:; \ct.C '31 crz.59: Sample Hardness %RPD= volume Begin End Total Multiplier (mg CaCOJIL) {(S - D) /[(S+D)/2]} X 100 ml ml ml s D Spike hardness (A) number Multiplier (mg CaC03/L) Sample hardness (B) (mg CaC03/L) Sam le measurements: Sample volume Begin End Total Hardness ml ml ml ml m CaCOJIL)
?'\D c~ l'i. rz \""l. at --*0\ 7.7 \(.0 3.3 \ct., '-S \ "l. ot ' &*o \ lt.G \5.5 '"l.S J, ~~
Note: If >15ml oftitrant is used, sample must be diluted. Page 69 of 99 Reviewed by: Date reviewed I 06 t91l-SOP C7 -Exhibit C7 .1. revision 06-01-11
Page ___._'f'-=--z.._ Page_,_ of_(_ Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pH/Ion Meter Analyst I iJ~ Iodi~e reagent: ~:r_N_~_s_u:~-----1 Acid reagent: 'IN<< 5\"1 Date analyzed :o=s-=*=l&.f=*=I'Z.======== ~~~~----------------~ Calibration: Lba oratory controI standar,: d l Reference standard True value (TV) Measured value (MV) %RS=MV /TVx 100 I number (mg/L) (mg/L) (acceptable range= 90 to 110%)
- !NSS
'0'"' 0.50 o.Sl-41 loS.i%
Sample characteristics %RPD = {(S- D) /[(S+D)/2]} X 100 (acceptable range= :1:: 10%) Sample ID Sample characteristics Note: All samples were analyzed in excess ofEPA recommended holding time (15 minutes) unless otherwise noted. L aboratory controI stan dar,d: Reference standard True value (TV) Measured value (MV) % RS=MV /TVx 100 number (mg/L) (mg/L) (acceptable range= 90 to 110%)
'J:NSS lO'"i 0.50 O .. c.f71 et'f.z..'tc, Reviewed by I ~-
Date reviewed ~===o:t\:.:,~:'"l=*~=:1...-====~ SOP C8 -Exhibit C8.1, revision 06-01-11 Page 70 of 99
Page cts I Page _l_. of _j___ Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pH/Ion Meter Analyst I~ Iodide reagent: ~-....;;J:N~tt_S_f_c_ _ _ _-1 Acid reagent: l:N t St? Date analyzed :o=S=*=tC=*=fl..======== ~----~------~ Calibration: Lb a oratory controI standard: Reference standard True value (TV) Measured value (MV) % RS=MV /TV X 100 number (mg/L) (mg/L) (acceptable range= 90 to 110%)
- J
- .J\)SS IOC"f 0.50 0.532
\0'*""'
Sample characteristics Duplicate D..Lo.oottS Sample ID Sample characteristics o.otttl LO.OfLil"f
<.o.ool3lf
'-O.oo ISl Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Lb a oratory controI standard: Reference standard True value (TV) Measured value (MV) % RS=MV /TV X 100 n*umber (mg/L) (mg/L) (acceptable range= 90 to 110%)
~N.S.S l GC...., 0.50 O.L(CS '\'f.o6~
Reviewed by I X Date reviewed :===o=r-t:* :,:\=,*=\=1-===: Page 71 of 99 SOP C8 -Exhibit C8.1, revision 06-01-11
Page '7 Page __L_ of_l_ Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg!L Meter: Accumet Model AR25 pHilon Meter Analyst ~-~__;;;;,_----~*~ Iodi~e reagent: ~:r____,;w;...;.R_SI~(-----1 Date analyzed o'i*\~*\~ . Actd reagent: :rtJ rt 5, 7
~~--~----------~
Calibration: Lb a oratory controI stan dard: Reference standard True value (TV) Measured value (MV) % RS=MV /TV X 100 number (mg!L) (mg/L) (acceptable range= 90 to 110%) J:l.)SS J06'f 0.50 0.520 \6'l.o~ Sample characteristics Duplicate SampleiD Sample characteristics LG.003ft: no Co Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. L aboratory controI standa11d: Referen.ce standard True value (TV) Measured value (MV) %RS=MV /TVx 100 . number (mg!L) (mg/L) (acceptable range= 90 to 110%)
- Z:J"l...g' l06Lf 0.50 o. 'iS"Cf C{l.'SY.
Reviewed by ~__,..V)t>>J..,;,..;;..:::~------1 Date reviewed OJ>i6-I 4 SOP C8 -Exhibit C8.1, revision 06-01-11 Page 72 of 99
Sequoyah Nuclear Plant Biomonitoring August 14 - 21, 2012 Appendix D Reference Toxicant Test and Control Chart Page 73 of 99
Pimephales promelas Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc. Environmental Testing Solutions, Inc. USEPA Control Limits(+/- 2 Standard Deviations) 0.9 0.8 ********** ********************************************************************************************* 0.7 0.6 ******************************************************************************************************** 0.5 1.4
-u
~
1.2 1.0 USEPA Warning and Control Limits (75th and 90th Percentile CVs)
"Qa u
in N 0.8
-> 0.6 "C "'
I 0.4 ******************************************************************************************************** 1.4 Laboratory Warning and Control Limits (lOth and 25th Percentile CVs) 1.2 1.0 0.8
~ .......
0.6 -**-**-**-**-**-**-**-**-**-**-** 0.4 Test date
- 7-day IC25 =25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
........ ....... Central Tendency (mean IC25 ) -**-**- Warning Limits (mean IC25 +/- sA.lO or sA.75) Graphs generated from associated excel spreadsheet. Excel spreadsheet entered by: J. Sumner
- Control Limits (mean IC25 +/- SA. 25, SA.90, or 2 Standard Deviations) Reviewed by:-~-
Page 74 of 99
~--~ ~-*--~ ---~--- ~-~ ~~ ~~ ~ ~ ~~~---- -* --~-~--~-------~~----~--- ------- - - * -~~ ~- ~ -- - -~----- - ----~--~"'
Page 75 of 99 Pimephales promelas Chronic Reference Toxicant Control Chart State and USEPA Laboratory Laboratory US EPA US EPA Test number Test date 7-day IC25 CT s Control Limits Warning Limits Control Limits Warning Limits SA.90 Control Limits cv (g/L KCI) (g/L KCI) CT-25 CT+2S CT- SA.lO CT + SA.lO CT- SA. 25 CT + SA.2S CT- SA.7s 08-09-11 0.67 0.71 0.05 0.62 0.80 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 2 09-13-11 0.66 0.71 0.05 0.61 0.80 0.08 0.62 0.79 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 3 10-04-11 0.73 0.71 0.05 0.61 0.81 0.09 0.62 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 4 10-11-11 0.76 0.71 0.05 0.62 0.81 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.03 0.07 5 10-18-11 0.67 0.71 0.05 0.61 0.81 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 6 11-08-11 0.78 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 7 12-06-11 0.77 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 8 02-07-12 0.77 0.71 0.05 0.61 0.81 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 9 02-14-12 0.74 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.04 0.07 10 03-06-12 0.64 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 11 03-06-12 0.68 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 12 04-12-12 0.71 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 13 05-08-12 0.77 0.72 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.08 14 05-15-12 0.71 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.04 0.08 15 06-05-12 0.72 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 16 06-12-12 0.77 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 17 07-10-12 0.76 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 18 07-17-12 0.73 0.72 0.05 0.62 0.81 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.07 19 08-07-12 0.70 0.72 0.05 0.62 0.81 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.07 20 08-14-12 0.81 0.73 0.05 0.63 0.82 0.09 0.64 0.81 0.15 0.57 0.88 0.28 0.45 1.00 0.33 0.40 1.05 0.06 Note: 7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. CT = Central tendency (mean IC25 ). S = Standard deviation of the IC25 values. Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. SA.1o =Standard deviation corresponding to the 1dh percentile CV. (SA.lo =0.12) SA. 25 = Standard deviation corresponding to the 25th percentile CV. (SA. 2s = 0.21) US EPA Control and Warning Limits SA. 7s = Standard deviation corresponding to the 75th percentile CV. ( SA.75 = 0.38) SA.90 = Standard deviation corresponding to the 90th percentile CV. (SA.90 = 0.45) CV = Coefficient of variation ofthe IC25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. File: ppkclcr_081412.xlsx Entered by: J. Sumner Reviewed by:~
Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Environmental Testing Solutions, Inc. Organism Source: Aquatox, Inc.
.c -~
CIJ ta 1.00 ... ... ... i0
~ ... ..!!!-; 0.75 0... :e .. ** ******************************************************************************************* ... +" .5 0.50 c ........
0 bO u E
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30 Kentucky Acceptance Limit(< 30.0%)
~ 3: *~
ta
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~
~ ********* ************* ********** *************************
'+- 0 ... **************************
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eCIJ *~ 0 0 u 30 USEPA Upper PMSD Bound (90th percentile< 30.0%} -~ 20 10
- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Growth, CV, or PMSD) Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) Graphs generated from associated excel spreadsheet. Excel spreadsheet entered by: J. Sumner Page 76 of 99 Reviewed by: ~
Precision of Endpoint Measurements Pimephales promelas Chronic Reference Toxicant Data Control Control Mean Test number Test date Survival Growth cr cv cr MSD PMSD cr for Control Growth for Control (%) (mg/larvae) (%) (%) for PMSD (%) (mg/larvae) Growth CV (%) 1 08-09-11 100 0.662 6.6 0.07 10.6 2 09-13-11 100 0.848 0.755 6.7 6.7 0.12 13.6 12.1 3 10-04-11 100 0.768 0.759 16.8 10.0 0.13 16.9 13.7 4 10-11-11 100 0.681 0.740 4.3 8.6 0.07 9.9 12.8 5 10-18-11 100 0.693 0.730 4.8 7.9 0.07 10.0 12.2 6 11-08-11 100 0.792 0.741 10.3 8.3 0.12 15.6 12.8 7 12-06-11 100 0.738 0.740 15.7 9.3 0.12 15.9 13.2 8 02-07-12 100 0.662 0.730 10.5 9.5 0.09 13.4 13.2 9 02-14-12 97.5 0.658 0.722 5.2 9.0 0.07 10.1 12.9 10 03-06-12 100 0.854 0.735 3.5 8.4 0.09 10.8 12.7 11 03-06-12 100 0.873 0.748 5.1 8.1 0.10 11.4 12.6 12 04-12-12 97.5 0.706 0.744 7.0 8.0 0.06 8.7 12.2 13 05-08-12 100 0.613 0.734 8.1 8.1 0.08 13.1 12.3 14 05-15-12 100 0.697 0.732 5.9 7.9 0.07 9.5 12.1 15 06-05-12 97.5 0.596 0.723 3.8 7.6 0.06 9.4 11.9 16 06-12-12 100 0.723 0.723 6.0 7.5 0.11 15.6 12.2 17 07-10-12 100 0.641 0.718 5.2 7.4 0.10 14.9 12.3 18 07-17-12 100 0.638 0.713 10.9 7.6 0.07 11.4 12.3 19 08-07-12 97.5 0.660 0.711 6.7 7.5 0.06 8.7 12.1 20 08-14-12 100 0.533 0.702 5.4 7.4 0.07 13.1 12.1 Note: CV = Coefficient of variation for control growth. Lower CV bound determined by USEPA (lOth percentile)= 3.5%. Upper CV bound determined by USEPA (90th percentile)= 20% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA {lOth percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile) = 30%. cr = Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. US EPA. 200la, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: ppkclcr_081412.xlsx Entered by: J. Sumner Reviewed by:-+ Page 77 of 99
Page I of5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas PpKClCR Test Number: ___2..5S Dilution preparation information: Comments: KCl Stock INSS number: .~s. ,02'\ Stock preparation: 50 gKCIIL: Dissolve 50 g KCl in 1-L Milli-Q water. Dilution prep (mg/L) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Test information: Organism age: "ll ~ '-\1. \o\a~s o..__b Randomizing template: (>~QR\.2. Date and times organisms 04* \'1>>* ,..,_ \'oCO Incubator number and were born between: shelf location: .3b Organism source:
~b~~Pp ()c. \'J. *l"'L Artemia CHM number: t..l\ tot t. ~ '"~;
Drying information for weight determination: Transfer vessel pH= , .. \,C s.u. Date I Time in oven: 6& .."l.\* \'L \~ ,I~ :'_:- information: Average transfer v9lume: Temperature = 0.\~d\~
,_Soc\ oc Initial oven temperature:
Date I Time out of oven: Co.b *t 6&*1.1... \'l.. \1.6tl 4oa *c..
.. Final oven temperature:
Total drying time: "\).\.** -""' Daily fe_eding and renewal information: Day Date Morning feeding Afternoon feeding MHSW batch used 0 2 3 4
- 11.-.
- 11...
SOP AT21- Exhibit AT21.1, revision 06-01-11 Page 78 of 99
r<<~_-.*_,_(_... Page 2 of5
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iET 6 Environmental Testing Solutions, Inc. I I Species: Pimephales promelas PpKClCR Test Number: ~S Survival and Growth Data I;. Day Control 450 m~_KCI/L 600 mg KCI/L I I ; 0
.A B c D E F G H I -! K L
~.:_ *8 1.~
/0 /0 /Q (() 10 IQ /Q I() 10 /0 10 IO 1
/0 10 10 /0 10 10 IO 10 IO lO /0 10 2
10 10 10 10 10 /C 10 10 10 10 10 tO
- ,_ 3 4
lD tO {0 to IO lC IC 10 lO IC
,a~
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/0 to (0 10 lO JC /0 10* '\ t\ (Q 10 7
10 'O /() /Q 10 '0 10 10 '1 '1 IQ 10 A= Pan weight (mg) ...
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~
Tray color code:: Analyst: t3.~~ 13.~ 14.gt.t \4.t3 13.88 IJ.43 14.~4 ~~.'J"L 13.81 t4.tn l3.bL rg.4z Date: 08*03*U.
/
B = Pan + Larvae weight (mg) / Analyst: .:1\.B ~.'2.7 Date: 08*2:7.1'2. tg,53' Z."r l.fo I'I.'Z."' 11.,6 -\1.l, 'Z.O.ff Zl.Sc:> lB. So 'Z.0.3'Z. l't.'*IO l't.6Z. C = Larvae weight (mg) = B -A s.1e c,.'-'1 t.f,C.'\ 4S.l.~ ,1.0 Hand calculated. Analyst: /'V-- s.""' s.O"'\ s.s\ 'S.\\ S.1\o (...'1'1 S.11
\..~
Weight per initial number of larvae (mg) .
= C I Initial number of larvae q .,.,.,
"\IJ b'0*,¢- ~ <<:;~ <<-;,
Hand calculated. Analyst: { j\],
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from control (%) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk = sick, sm =unusually small, I lg = unusually large, d&r = decanted and returned, w = wounded. Comments: I I
~
SOP AT21 - Exhibit AT21.1, revision 06-01-11 Page 79 of 99
- Page 3 of5 Species: Pimephales promelas PpKCICR Test Number: 1.S~
Survival and Growth Data Day 750 m~ KCIIL 900 mg I(CIIL 1050 m! KCIIL M N 0 p Q R s T u v w X 0 JD /0 IO IO /0 IO IO 10 /Q IO 10 10 1
/0 tO /0 tO .B 1IA **M 1 {A 'ItA . 1-'.
3 --\'-' '-\~ ~ . .,. 2 g ~~ '-\ *q 3
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I Tray color code:: Analyst: t3.\t 14.sc.. '3.lB lZ.gt, f4.S'\ '~*t'Z .IZ.IB 14.11 IZ.B~ 13.t8 '3~o4 13.Z.-6 Date: o8*03*~l. B =Pan + Larvae weight (mg) u; Analyst: Date:
.,-ur*
()1f*"Z. ..,,,z. 11."i4f 1'1. 'Z."' 1s. '31 17.49' 17.,, 17.ZS \G.'Z..l 17.30 \ 3."3, t"'.76 ~ I. C = Larvae weight (mg) = B- A
~ ,
.... 'l.c.a '1.~~ s.t"l. ~.10 ~.9o "(."<:6 ~.\~ o.~\ a.s& .....
i Hand calculated. ~ ** F,,,f_._*i~ Analyst: * .-......---' r f: Weight per initial number of larvae (mg) 1 1 ¥~.:_*
= C I Initial number of larvae
'" !\ ~ ~ §l ~ ~1$1. J' *~ ~ c 0
~ ~
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Hand calculated. (,) ~ ~
~
Analyst: -- o* o* (). tl. o* t;;l o* o* ~*
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Average weight per initial Percent number oflarvae (mg) reduction 0 ."118' JQ. ':,?. o.~& 3l\.t'1. o.os1. 16* 2 7. I' from control (%) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk = sick, sm =unusually small, i: lg = unusually large, d&r = decanted and returned, w = wounded. i_*.*.*_,_-_*.*. I Comments: I ; .**.*_****,* I~i( Page 80 of 99 SOP AT21 -Exhibit AT21.1, revision 06-01-11
Page 81 of 99 Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: _ _ _ _ _ _ _ _ _ _ _ _ 25_5 Test dates: _ _ _ _ _ _ _A_u:.gu_s_t_14-_2_1..:.,_20_1_2 Concentration (mc/L KO) Repllcete lnltlal number of Rnal number of A= Pan wellht (mg) B=Pan+larvae Larvae weight (mg) = Weight I Surviving Mean weight/ Surviving Coafllclent of variation Weight I lnltlal number of Mean survival (") Mean weight/ Jnltlal Coefficient of variation Percent reduction from larvae larvae welght(mg) A*B number of larvae (mg) number of larvae (m1) (Men wetcht per suMvlrc larvae(mg) number of larvae I"I control l"l numberoflo,.vaeJ(") (mg) A 10 10 13.63 19.27 5.64 0.564 0.564 B 10 10 13.46 18.53 5.07 0.507 0.507 Control 0.533 5.4 100.0 0.533 5.4 Not applicable c 10 10 14.89 20.40 5.51 0.551 0.551 D 10 10 14.13 19.24 5.11 0.511 0.511 E 10 10 13.88 19.66 5.78 0.578 0.578 F 10 10 13.43 19.19 5.76 0.576 0.576 450 0.607 5.9 100.0 0.607 5.9 -13.9 G 10 10 14.54 20.81 6.27 0.627 0.627 H 10 10 15.02 21.50 6.48 0.648 0.648 I 10 9 13.81 18.50 4.69 0.521 0.469 J 10 9 14.69 20.32 5.63 0.626 0.563 600 0.586 8.2 95.0 0.558 11.4 -4.5 K 10 10 13.62 19.40 5.78 0.578 0.578 L 10 10 13.42 19.62 6.20 0.620 0.620 M 10 8 13.12 17.89 4.77 0.596 0.477 N 10 9 14.56 19.24 4.68 0.520 0.468 750 0.547 7.9 87.5 0.478 5.2 10.5 0 10 9 13.78 18.31 4.53 0.503 0.453 p 10 9 12.86 17.98 5.12 0.569 0.512 Q 10 5 14.59 17.69 3.10 0.620 0.310 R 10 6 13.72 17.28 3.56 0.593 0356 900 0.607 4.4 57.5 0.348 12.4 34.8 s 10 7 12.18 16.23 4.05 0.579 0.405 T 10 5 14.11 17.30 3.19 0.638 0.319 u 10 1 12.85 13.36 0.51 0.510 0.051 1050 v 10 2 13.18 14.76 1.58 0.790 0.650 30.5 0.158 7.5 0.052 142.6 90.2 w 10 0 0.00 0.00 0.00 0.000 0.000 X 10 0 0.00 0.00 0.00 0.000 0.000 Dunnett's MSD value: 0.0699 MSD= Minimum Significant Difference PMSD: 13.1 PMSD= Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10th percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile)= 30%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (US EPA, 200la; USEPA, 200lb). USEPA. 200la, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: ppkclcr_081412.xlsx Entered by: J. Sumner Reviewed b y : - #
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 8/14/2012 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mg/L 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 600 0.9000 0.9000 1.0000 1.0000 750 0.8000 0.9000 0.9000 0.9000 900 0.5000 0.6000 0.7000 0.5000 1050 0.1000 0.2000 0.0000 0.0000 Transform: Arcsin Sguare Root Rank 1-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 600 0.9500 0.9500 1.3305 1.2490 1.4120 7.072 4 14.00 10.00 2 40
*750 0.8750 0.8750 1.2136 1.1071 1.2490 5.846 4 10.00 10.00 5 40
*900 0.5750 0.5750 0.8620 0.7854 0.9912 11.405 4 10.00 10.00 17 40
*1050 0.0750 0.0750 0.2757 0.1588 0.4636 53.294 4 10.00 10.00 37 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93302 0.884 0.39148 0.28113 Eguality of variance cannot be confirmed Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 600 750 670.82 Treatments vs D-Control Trimmed Spearman-Karber Trim Level EC50 95%CL 0.0%
5.0% 10.0% 902.19 867.55 938.21 1.0 - . - - - - - - - - - - - - - - - - - . 20.0% 909.49 869.16 951.70 0.9 Auto-7.5% 898.26 863.27 934.67 0.8 0.7 8! c 0.6 8.. 0.5
~ 0.4 0.3 0.2 0.1 J 0.0 +--r-r"TTTmr---.-rrrrmr--r'"T'4fnmr-"1""T"T-rrml 1 10 100 1000 10000 Dose mg/L Dose-Response Plot 0.9 0.8 I'CI 0.7
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Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 8/14/2012 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mg/L 2 3 4 D-Control 0.5640 0.5070 0.5510 0.5110 450 0.5780 0.5760 0.6270 0.6480 600 0.4690 0.5630 0.5780 0.6200 750 0.4770 0.4680 0.4530 0.5120 900 0.3100 0.3560 0.4050 0.3190 1050 0.0510 0.1580 0.0000 0.0000 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5333 1.0000 0.5333 0.5070 0.5640 5.353 4 0.5703 1.0000 450 0.6073 1.1388 0.6073 0.5760 0.6480 5.924 4 -2.307 2.180 0.0699 0.5703 1.0000 600 0.5575 1.0455 0.5575 0.4690 0.6200 11.433 4 -0.756 2.180 0.0699 0.5575 0.9776 750 0.4775 0.8955 0.4775 0.4530 0.5120 5.244 4 0.4775 0.8374 900 0.3475 0.6517 0.3475 0.3100 0.4050 12.430 4 0.3475 0.6094 1050 0.0523 0.0980 0.0523 0.0000 0.1580 142.558 4 0.0523 0.0916 Auxllia~ Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94603 0.805 -0.6553 0.58729
=
Bartlett's Test indicates egual variances (!;! 0.40} 1.85289 9.21035 H:t~othesis Test {1-tail, 0.05~ NOEC LOEC ChV TU MSDu MSD~ MSB MSE F-Prob df Dunnett's Test 600 >600 0.06992 0.13112 0.00569 0.00206 0.11571 2,9 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95% CL(Ex~) Skew iC05 629.55 44.05 422.75 677.99 -1.2511 IC10 683.02 39.17 471.16 745.11 -1.6458 IC15 736.48 32.27 546.98 788.00 -2.5024 1.0 . . . . . . - - - - - - - - - - - - - - . IC20 774.58 14.39 725.29 814.40 0.0156 0.9 IC25 807.48 14.81 763.34 852.44 0.1502 0.8 IC40 902.72 14.24 850.75 928.37 -0.5101 IC50 931.69 9.08 905.24 958.94 0.1578 0.7 0.6
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*Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table locat~on Page Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:
SOP AT21 - Exhibit AT21.1, revision 06-01-11 Page 84 of 99
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6 Environmental Testing Solutions, Inc. Page 5 of5 Species: Pimephales promelas PpKCICR Test Number: -'2.SS CONTROL
. 450 mg KCI/L 600 mgKCI/L 750 mgKCI/L 900 mgKCIIL
*Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily R~newal Information table locate~n Page 1.
I Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:
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I SOP AT21- Exhibit AT21.1, revision 06-01-11 j* Page 85 of 99
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart 1.14 USEPA Control Limits (+/- 2 Standard Deviations) 1.12 1.10 1.08 **************************** 1.06 1.04 **. ................... ************************************************************** 1.02 2.5 Q- USEPA Warning and Control Limits (75th and 90th Percentile CVs) u 2.0 z= ...............................................................................................
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- 7-day IC25 = 25% inhibition concentration. An estimation ofthe concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
- - . Central Tendency (mean IC 25 ) _ .. _ .. _ Warning Limits (mean IC25 +/- SA 10 or SA 75 ) .. * .. . . . . Control Limits (mean IC25 +/- SA25 , SA90, or 2 Standard Deviations) Graphs generated from associated excel spreadsheet. Excel spreadsheet entered by: J. Sumner Page 86 of 99 Reviewed by:~
Page 87 of 99 Ceriodaphnia dubio Chronic Reference Toxicant Control Chart State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day IC25 CT s Control Limits Warning Limits SA.25 Control Limits SA.1s Warning Limits Control Limits cv (g/L NaCI) (g/L NaCI) CT-25 CT+2S CT - SA.lo CT + SA.lo CT - SA.9o CT + SA.90 1 04-05-11 1.07 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 2 05-03-11 1.07 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 3 06-07-11 1.05 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 4 07-12-11 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 5 08-09-11 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 6 09-13-11 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 7 10-04-11 1.07 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 8 10-11-11 1.06 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 9 11-08-11 1.05 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.01 10 12-06-11 1.06 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 11 01-10-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.01 12 02-07-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 13 03-13-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 14 04-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 15 04-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01 16 05-08-12 1.06 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01 17 05-08-12 1.05 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 18 06-05-12 1.05 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 19 07-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01 20 08-07-12 1.08 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 Note: 7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC25 ). 5 =Standard deviation ofthe IC25 values. Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. SA.lo =Standard deviation corresponding to the 10th percentile CV. (SA.1o =0.08) SA.25 =Standard deviation corresponding to the 25th percentile CV. (SA.25 =0.17) USEPA Control and Warning Limits SA.75 =Standard deviation corresponding to the 75th percentile CV. (SA.7s =0.45) SA.90 =Standard deviation corresponding to the 90th percentile CV. (SA.9o =0.62) CV = Coefficient of variation of the IC25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, ancinnati, OH. File: CdNaCICR 080712.xlsx Entered by: J. ~er Reviewed by:
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements 40 ...'t= Q
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Central Tendency (mean Control Reproduction, CV, or PMSD) Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations) Graphs generated from associated excel spreadsheet. Excel spreadsheet entered by: J. Sumner Page 88 of 99 Reviewed b y : +
** Precision of Endpoint Measurements Environmental Testing Solutions, Inc.
Ceriodaphnia dubio Chronic Reference Toxicant Data Test Control Control Mean number Test date Survival Reproduction CT cv CT MSD PMSD CT for Control Mean for Control (%) (offspring/female) Reproduction (%) Reproduction (%) forPMSD (%) (offspring/female) CV(%) 1 04-05-11 100 33.0 5.9 2.2 6.7 2 05-03-11 100 30.9 32.0 4.5 5.2 1.9 6.1 6.4 3 06-07-11 100 28.9 30.9 6.7 5.7 2.5 8.7 7.2 4 07-12-11 100 32.2 31.3 6.0 5.8 2.0 6.2 6.9 5 08-09-11 100 28.3 30.7 5.0 5.6 1.9 6.8 6.9 6 09-13-11 100 32.1 30.9 8.2 6.1 1.9 5.8 6.7 7 10-04-11 100 32.8 31.2 6.1 6.1 2.1 6.5 6.7 8 10-11-11 100 31.3 31.2 3.0 5.7 2.1 6.6 6.7 9 11-08-11 100 29.0 30.9 7.6 5.9 1.8 6.1 6.6 10 12-06-11 100 29.3 30.8 5.8 5.9 1.9 6.5 6.6 11 01-10-12 100 32.4 30.9 5.1 5.8 2.4 7.4 6.7 12 02-07-12 100 31.7 31.0 4.2 5.7 2.1 6.5 6.7 13 03-13-12 100 31.1 31.0 7.2 5.8 1.9 6.1 6.6 14 04-10-12 100 29.4 30.9 6.8 5.9 2.4 8.0 6.7 15 04-10-12 100 30.3 30.8 7.1 6.0 2.7 8.8 6.8 16 05-08-12 100 31.7 30.9 6.1 6.0 1.9 6.1 6.8 17 05-08-12 100 32.4 31.0 5.1 5.9 2.0 6.1 6.8 18 06-05-12 100 32.1 31.1 4.0 5.8 2.3 7.1 6.8 19 07-10-12 100 31.2 31.1 4.7 5.8 2.0 6.3 6.8 20 08-07-12 100 29.2 31.0 3.5 5.6 1.9 6.6 6.7 Note: CV = Coefficient of variation for control reproduction. Lower CV bound determined by USEPA (lOth percentile)= 8.9%. Upper CV bound determined by USEPA (90th percentile)= 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (lOth percentile) = 13%. Upper PMSD bound determined by US EPA (90th percentile) = 47%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) US EPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. US EPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: CdNaClCR_ 080712.xlsx Entered by: J. Sumner Page 89 of 99 Reviewed b y : +
Page 1 of6 . Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia CdNaCICR #: __;,;;l~--~.;::_ I1"'*. **
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100 g NaCl/L: Dissolve 50 g NaCl in 500 mL Milli-Q water. 800 1000 1200 1400 Comments: I* Stock volume (mL) Diluent volume (mL) 1491 9 12 1488 15 1485 18 1482 21 1479 Total volume_(mL) 1500 1500 1500 1500 1500 Test organism source information: Test information: Organism age: < 24-hours old Randomizing template color: &ot.h Date and times organisms were born at*cn-*1- 0"\~'TO lObO Incubator number and shelf between: location: 1.1>\ Culture board: 0"\*&\*ll.... J\ Replicate number: I 2 13 14 1s 16 11 1s 19 10 YWTbatch: Culture board cup number: \ 11.. I I I "\ 110 I ll I 1'\ I "2.0 ll't "5'1.. ()fo< tO.* *1..... Transfer vessel information: pH =,.1r,1 S.U. Temperature = 1,\l,'l oc Selenastrum batch: Average transfer volume (mL): o. 0~11 .-..Q. .. 01~1* J"l.... Daily renewal information: Day Date Test initiation and feeding, MHSW. Analyst renewal and feeding, or batch used termination time 0 2 3 4 5 6 7 Control information: Acceptance criteria Summary of test endpoints:
% of Male Adults: 07. ::;;20% 7-day LCso :>1&400
%Adults having 3rd Broods: f[)bl. ;?!:80% NOEC 1000
% Mortality: 07. ::;;20% LOEC l2ntl Mean Offspring/Female: "2.1. 2. ;?!: 15.0 offspring/female ChV IO'i~. -s
%CV: -~*~1 .. <40.0% IC2s 1011. 3 SOP AT14 -Exhibit AT14.1, revision 06-01-11 Page 90 of 99
I !ETS Page2 of6 _ 0 En>tronmentaiTosUng Solutions, Inc. J Species: Ceriodaphnia dubia CdNaCICR #: ~ ~f I CONTROL 1 Survival and Reproduction Data Replicate number Day ( 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 0 c 0 c 0 Adult mortality \._... \...... L.. L.. L- '-- L L. L- \....._ 2 Young produced t'l Cl -~ c 0 c a 0 0 C) Adult mortality \._...
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Mean Offspring/Female: I "Z.1.~ 600 mg NCIIL a S urvzva . I an dR eproduct*zon D ata Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced c L. 0 0 0 0 0 0 0 () 0 Adult mortality L L l L L L L L L 2 Young produced c 0 0 *o c (J 0 0 0 0 Adult mortality *t L L L L L L. L L L 3 Young produced () a 0 0 0 {) *C 0 0 0 Adult mortality L L '- L L L. 'L \... \.._ L 4 Young produced 5 .s --{ \. to '"'\ '-L ~ ~
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- Concentration:
SOP AT14- Exhibit AT14.1, revision 06-01-11 Page 91 of 99
iETS Page 3 of6 6 EnvlronmentoiTostlngSolutlons.lnc. Species: Ceriodaphnia dubia CdNaCICR #: \'!>8 800 m2 Na CI/L Survzva . I an dReproduct"zon D at a Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced a c 0 () 0 u 0 0 0 a Adult mortality
'- '- L '-- L- L... L '--
L- '-- 2 Young produced D 0 A I""\ {"\ t'\ (\ c 0 0 Adult mortality L \..... \.....- L.. L... '-- '-.... '--- '-... '-- 3 Young produced 0 D 0 0 0 0 c () 0 0
\..._ \._ \...._ L- \.._
Adult mortality
'-- L.. '-- L-* '--
4 Young produced & S> '-\ '"\ 'l 5 '4 ~ '-~ s Adult mortality L '-- '- '- '-- '--- '-- L... L..... 5 Young produced \\ \\ lC \\ \0 \'2.. IC \~ \1.. IC Adult mortality L '- \....... L '-- '-- \...... 'L.. L- '-- 6 Young produced c D 0 b D a 0 0 (j () Adult mortality
'- \..... '\...._
\.... \...... '-- \...... '-- L '--
\'-\ \"'"\ 's 7
Total young produced Young produced
- !>~ :;o ,.
14 '~ \'!>
~a "Z.,
\\o 3~ "2.'\ ~Co
\\
L.
\~
'!.\ l.C\
'\\..(
Final Adult Mortality
'-- \.. '- \...._ \..... L '-
Note: Adult momtlity (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offspnng
'- L carried over with adult during transfer).
Concentration:
% Mortality: 07.
Mean Offspring/Female: 30.\
-~**
% Reduction from Control:
1000 m2 NCIIL a Survzva . I andReproduct*zon D ata Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 0 0 0 0 ('J (j Adult mortality \.._ L L L L L L L L L 2 Young produced 0 c a 0 a (J C) ~ 0 c Adult mortality L L L L l L l L.... L L 3 Young produced 0 0 a (\ a (\ *(') L (') 0 0 Adult mortality L L.. L L L L- L- L- L 4 Young produced s '4. (o' \w\ '-\ \.\ '-\ '3 ~t 4 Adult mortality L L '-- L L L l- L L \._ 5 Young produced 0 \L 1\ t\ tD 'b 10 10 10 10 Adult mortality L '- '- L. L L \.._ L... '- L 6 Young produced t\ 0 c D 0 0 c 0 0 t5 Adult mortality \...~o- '- L \..... '-.. \.... \._. L \..., L 7 Total young produced Young produced \5
~\
'S
!>>\
\'!;
!.b
'S
~t) l'"Z..
2C.
\"\
2.8
'~
,_, t~*
\~* \'-\'
'l.&
Final Adult Mortality '-- \..... L \. \. "-- \.._ L L.. '- Note: Adult mortahty (L = hve, D =dead), SB = spht prood (smgle brood spht between two days), CO= carry over (offsprmg carried over with adult during transfer). Concentration:
% Mortality: 01.
Mean OffsprinWFemale: 28.'1
% Reduction .from Control: *z..17.
SOP AT14- Exhibit AT14.1, revision 06-01-11 Page 92 of 99
Page 4 of6 CdNaCICR#: mg a surv1va
- I and Reproduction Data Replicate number Day 1 2 .3 4 5 6 7 8 9 10 1 Young produced Q 0 0 0 0 0 0 Q c 0 Adult mortality \.._ \.... L- '-- '-- L.. L- \..._ \..._ L 2 Young produced c 0 c 0 *c c 0 0 0 tJ Adult mortality
' - '-- \._... L \..... \....... \,..... L. '-- L_
3 Young produced t) a () a a (') C) a (J
.L
() Adult mortality \.... L- '-- \... \._
'-- \..._ L '---
4 Young produced .~ \ L '2- .3. .2..* \ ~ !. ~ 5 Adult mortality Young produced Adult mortality 0 Cc
\.....
'-\ s
\.....
L L L s
\....
L
\.
li
\......
s L-
\ \..... '- L.. \........ \...._
6 Young produced a '\ 0 c () a D D c c 7 Adult mortality Young produced '-l L '-- '--
'-- 5
\........
~ .s L-
, L. '--
~
'-\ S_
Total young produced I'-\ . ~~ '\ l~ 12.. \'!> \\:. (!, Final Adult Mortality '"'
'- '- '-- '-:.. '- '- '-- . '-- \..._ '-
Note: Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offspnng
\!:,
carried over with adult during transfer). Concentration:
% Mortality: 07 ..
Mean Offspring/Female: \'!a~,..
% R~duction from Control: s'-\.2 1400 mg NCIIL a Surv1va . I andR eproduct*zon D ata Re_plicate number Day 1 2 3 4 5 '6 7 8 9 10 1 Young produced 0 Q 0 0 D {) D _0 0 0 Adult mortality \....... \...... L L L L L l- L L-2 Young produced 0 D 0 0 0 D c 0 0 _Q Adult mortality *L L. L L L L... L L L L-3 Young produced 0 0 0 0 () 0 '() 0 0 0 Adult mortality L L L L. L L L L. L... L 4 Young produced l \ \ '1.. 1_ 6 '2.. 3 ~ \
Adult mortality L \..... L L L L L L L. L.. 5 Young produced 0 (:) '1.. ..3 (J '"1.. '"-\ \ ~ '-
. Adult mortality L L L \....... \....... L L. \...- L.. L Young produced ~ 0 D "'L* 0 0 0 c 0 6
Adult mortality
'- \.... '-- '-- \..._ \..._ \...... \...._
'-- L.
7 Young produced Total young produced
-z...
~
"L \
'-\ \.
\
' 3 1...
~ \
~
\, .
'5 Final Adult Mortality \.. '-- L. '-- L ~ L \..... '-
carried over with adult during transfer). Note: Adult mortality (L = hve, D =dead), SB = spht brood (smgle brood spht between two days), CO= carry over (offspnng Concentration: emale:
% Reduction from Control:
SOP AT14 -Exhibit AT14.1, revision 06-01-11 Page 93 of 99
~* Page 94 of 99
*
- Environmental Testing Solutions, Inc.
Verification of Ceriodaphnia Reproduction Totals Control 1000 mg NaCI/L Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 4 5 5 6 4 5 5 47 4 5 4 6 4 4 4 4 3 4 4 42 5 11 0 10 10 10 10 12 10 10 10 93 5 0 12 11 11 10 10 10 10 10 10 94 6 0 10 0 0 0 0 0 0 0 0 10 6 11 0 0 0 0 0 0 0 0 0 11 7 15 13 15 15 14 15 13 16 13 13 142 7 15 15 13 15 12 14 13 13 13 14 137 Total 30 28 29 29 29 30 31 30 28 28 292 Total 31 31 30 30 26 28 27 26 27 28 284 600 mg NaCIIL 1200 mg NaCIIL Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 4 6 6 4 6 5 5 5 51 4 3 1 2 2 3 2 1 3 3 3 23 5 11 0 10 10 13 11 11 10 13 10 99 5 7 0 6 4 5 8 5 6 9 5 55 6 0 11 0 0 0 0 0 0 0 0 11 6 0 9 0 0 0 0 0 0 0 0 9 7 14 13 14 14 14 16 16 14 13 13 141 7 4 6 5 3 5 2 7 4 4 5 45 Total 30 29 28 30 33 31 33 29 31 28 302 Total 14 16 13 9 13 12 13 13 16 13 132 800 mg NaCI/L 1400 mg NaCI/L Replicate number Re})licate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 4 4 4 5 4 6 6 5 48 4 1 1 1 2 2 3 2 3 3 1 19 5 11 11 10 11 10 12 10 13 12 10 110 5 0 0 2 3 0 2 4 1 2 2 16 6 0 0 0 0 0 0 0 0 0 0 0 6 1 3 0 0 2 0 0 0 0 0 6 7 14 14 14 13 13 16 15 17 13 14 143 7 2 2 1 1 1 3 2 3 1 3 19 Total 30 30 28 28 27 33 29 36 31 29 301 Total 4 6 4 6 5 8 8 7 6 6 60 File: CdNaCICR_080712.xlsx Entered by: J. Sumn(3r Reviewed by: -Jf-
. *~
Page 95 of 99
*** Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Environmental Testing Solutions, Inc.
Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #138
----------------------~-
Test dates: _____________ A_ug=-u_s_t_07_-_14_;,_2_0_12_ Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (%) (offspring/female) variation (%) control (%) (mg/LNaO) 1 2 3 4 5 6 7 8 9 10 Control 30 28 29 29 29 30 31 30 28 28 100 29.2 3.5 Not applicable 600 30 29 28 30 33 31 33 29 31 28 100 30.2 6.0 -3.4 800 30 30 28 28 27 33 29 36 31 29 100 30.1 8.9 -3.1 1000 31 31 30 30 26 28 27 26 27 28 100 28.4 6.9 2.7 1200 14 16 13 9 13 12 13 13 16 13 100 13.2 15.1 54.8 1400 4 6 4 6 5 8 8 7 6 6 100 6.0 23.6 79.5 Dunnett's MSD value: 1.929 MSD = Minimum Significant Difference PMSD: 6.6 PMSD = Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effiuent toxicity test. Lower PMSD bound determined by USEPA (lOth percentile)*= 13%. Upper PMSD bound determined by USEPA (90th percentile)= 47%. Lower and upper PMSD bounds were determined from the lOth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200la; USEPA, 2001b). USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effiuent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: CdNaCICR_080712.xlsx Table populated from associated Verification of Ceriodaphnia Reproduction Totals" spreadsheet. Spreadsheet entered by: J. Sumner Reviewed by:-<:/(--
Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 8n12o12 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 8/14/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mg/L 2 3 4 5 6 7 8 9 10 D-Control 30.000 28.000 29.000 29.000 29.000 30.000 31.000 30.000 28.000 28.000 600 30.000 29.000 28.000 30.000 33.000 31.000 33.000 29.000 31.000 28.000 800 30.000 30.000 28.000 28.000 27.000 33.000 29.000 36.000 31.000 29.000 1000 31.000 31.000 30.000 30.000 26.000 28.000 27.000 26.000 27.000 28.000 1200 14.000 16.000 13.000 9.000 13.000 12.000 13.000 13.000 16.000 13.000 1400 4.000 6.000 4.000 6.000 5.000 8.000 8.000 7.000 6.000 6.000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 29.200 1.0000 29.200 28.000 31.000 3.537 10 29.833 1.0000 600 30.200 1:0342 30.200 28.000 33.000 6.005 10 121.00 75.00 29.833 1.0000 800 30.100 1.0308 30.100 27.000 36.000 8.921 10 111.50 75.00 29.833 1.0000 1000 28.400 0.9726 28.400 26.000 31.000 6.884 10 92.00 75.00 28.400 0.9520
*1200 13.200 0.4521 13.200 9.000 16.000 15.067 10 55.00 75.00 13.200 0.4425
*1400 6.000 0.2055 6.000 4.000 8.000 23.570 10 55.00 75.00 6.000 0.2011 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01) 1.17686 1.035 0.53863 0.89662
=
Bartlett's Test indicates equal variances Cp 0.14) 8.40619 15.0863 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 1000 1200 1095.45 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95% CL Skew IC05 1000.77 44.7051 868.009 1015.87 -1.6465 IC10 1020.39 9.61319 991.422 1034.38 -1.3975 IC15 1040.02 7.68096 1020.26 1053.25 -0.3510 1.0 . . . . . - - - - - - - - - - - -..... IC20 1059.65 7.19436 1041.68 1071.64 -0.3336 0.9 IC25 1079.28 6.83735 1063.08 1090.45 -0.3157 IC40 1138.16 6.71529 1123.99 1148.74 -0.2616 0.8 IC50 1177.41 7.42545 1162.25 1189.63 -0.2048 0.7 3! 0.6 s Q. 0.5 K! 0.4 0:: 0.3 0.2 0.1
. -=--: - .-=----~-~_-_ _~"';.
0.0 . - -........
~.1 +-~~~,-~~~,-~~~~
0 500 1000 1500 Dose mg/L Dose-Response Plot 40 35 30 ts0 25
- J
'..8 20 Q.
~ 15 10 5
0
- gc: 0 0
co 0 0 0 0 0 0 0
..,.0
~ C'll CX) 8 r r c File: CdNaCICR_080712.xlsx Entered by: J. Sumner Reviewed by:~
Page 96 of 99
Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 8/7/2012 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 8/14/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Used for PMSD calculation only. Conc-mg/L 1 2 3 4 5 6 7 8 9 10 D-Control 30.000 28.000 29.000 29.000 29.000 30.000 31.000 30.000 28.000 28.000 600 30.000 29.000 28.000 30.000 33.000 31.000 33.000 29.000 31.000 28.000 800 30.000 30.000 28.000 28.000 27.000 33.000 29.000 36.000 31.000 29.000 1000 31.000 31.000 30.000 30.000 26.000 28.000 27.000 26.000 27.000 28.000 1200 14.000 16.000 13.000 9.000 13.000 12.000 13.000 13.000 16.000 13.000 1400 4.000 6.000 4.000 6.000 5.000 8.000 8.000 7.000 6.000 6.000 Transform: Untransformed 1-Tailed Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 29.200 1.0000 29.200 28.000 31.000 3.537 10 600 30.200 1.0342 30.200 28.000 33.000 6.005 10 -1.186 2.287 1.929 800 30.100 1.0308 30.100 27.000 36.000 8.921 10 -1.067 2.287 1.929 1000 28.400 0.9726 28.400 26.000 31.000 6.884 10 0.948 2.287 1.929
*1200 13.200 0.4521 13.200 9.000 16.000 15.067 10 18.969 2.287 1.929
*1400 6.000 0.2055 6.000 4.000 8.000 23.570 10 27.505 2.287 1.929 AuxiliaryTests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01) 1.17686 1.035 0.53863 0.89662 Bartlett's Test indicates egual variances (p = 0.14) 8.40619 15.0863 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 1000 1200 1095.45 1.92879 0.06605 1109.51 3.55741 1.6E-38 5, 54 Treatments vs D-Control File: CdNaCICR_080712.xlsx 4
Entered by: J. Sumner Reviewed by: Page 97 of 99
Page 5 of6 Species: Ceriodaphnia dubia CdNaCICR#: CONTROL 600 mg NaCI/L 800 mg NaCI!L 1200 mg NaCIIL STOCK I *Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal i Information table located on Page 1. Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: 6'{ . . I'.
- . t:**.*.
I' Page 98 of 99 SOP AT14- Exhibit AT14.1, revision 06-01-11
Page 6 of6 Species: Ceriodaphnia dubia CdNaCICR#: CONTROL 600 mg NaCIIL 800 mg NaCIIL 1200 mg NaCIIL 1400 mg NaCIIL
*Temperatures performed at the time oftest initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: d( .
SOP AT14- Exhibit AT14.1, revision 06-01-11 Page 99 of 99
S58 12111 0 800 - NPDES CORRESPONDENCE November 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SON)- NPDES PERMIT NO. TN0026450- DISCHARGE MONITORING REPORT (DMR) FOR OCTOBER 2012 Enclosed is the October 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The turbine building sump (TBS) discharged directly to the yard drainage pond during the reporting period and was monitored in accordance with the narrative condition found in Part 1.A.2. of the subject permit. Enclosed is TBS monitoring data as well as attachments describing two collection system overflows that occurred during the reporting period. At no time was there a threat to public drinking supplies, to human health, or the environment. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Signatory Authority for: John T. Carlin Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D. Booker, MOB 1 F-WBN J.W. Proffitt, OPS 4C-SQN B. E. Brickhouse, BR 4A-C A. A. Ray, WT 11A-K J. T. Carlin, OPS 4A-SQN G. R. Signer, WT 6A-K J. A. Cross, POB 2A-SQN P.R. Simmons, POB 2B-SQN T.R. Markum, BR 4A-C B. N. Smith (EDMS), MPB 1 E-M D. B. Nida, BR 4A-C
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 November 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- DISCHARGE MONITORING REPORT (DMR) FOR OCTOBER 2012 Enclosed is the October 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The turbine building sump (TBS) discharged directly to the yard drainage pond during the reporting period and was monitored in accordance with the narrative condition found in Part 1.A.2. of the subject permit. Enclosed is TBS monitoring data as well as attachments describing two collection system overflows that occurred during the reporting period. At no time was there a threat to public drinking supplies, to human health, or the environment. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Q?t~~ Plant Manager Signatory Authority for: John T. Carlin Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013
TVA Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 1 Description of the Event and Determination of Cause On 10/19/2012 at 04:25 EDT the Sequoyah (SQN) Shift Manager was notified of a sewage overflow coming from a manhole located on the west side of the SQN Cafeteria. The shift manager initiated the SQN Spill Plan and dispatched personnel to investigate. A slight stream of sewage was discovered flowing out of the manhole ancl running approximately 50 feet down an access road before it reached a yard drain. On 10/19/2012 at 04:40 EDT the sewage overflow was stopped by cycling the number two pump from auto to manual and pumping the manhole down. Preliminary information regarding the overflow was communicated by telephone to the Chattanooga Environmental Field Office on 10119/2012 at 15:03 EDT. The total quantity of sewage which overflowed into a yard drain was estimated at less than 5 gallons. The initial investigation did not reveal any interruptions in power which could have caused the number two pump to trip in the off position. The cause of the overflow was attributed to partial clogging of the number two pump. This manhole is also where the cafeteria grey water enters the sewer system. At this time SQN is in a refueling outage and the partial clogging of the number two pump was caused by increased usage of the sewer system by support personnel and continuous cafeteria usage. All remaining sewage residue in the access road was neutralized with lime. The yard drain flows to the SQN Yard Drainage Pond, which discharges to the Diffuser Pond. The Diffuser Pond discharges through Outfall 101 to the Tennessee River. At no time was there a threat to public drinking supplies, to human health, or the environment. Period of Discharge The shift manager was notified of the overflow on 10/19/2012 at 04:25 EDT. The sewage overflow had just reached the yard drain as personnel responding to the spill arrived. The number two sewage ejector pump was restarted on 10/19/2012 at 04:40 EDT and this action immediately stopped the overflow. and discharge to the yard drain. The estimated time of discharge was less than 15 minutes. Steps Being Ta.ken to Reduce, Eliminate, and Pr~vent Recurrence This incident was entered into the TVA Corrective Action Program. Sequoyah has implemented shiftly monitoring of this sewage ejector location as an added precaution for the remaining duration of the outage to prevent another overflow.
TVA Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 2 Description of ~he Event and Determination of Cause On 10/27/2012 at 23:00 EDT Sequoyah (SQN) Shift Manager was notified of a high level alarm and a small amount of water coming out of the control panel for the turbine building sewage ejector pump controls. The shift manager initiated the SQN Spill Plan and dispatched personnel to investigate. *On 10/27/2012 at 23:15 EDT a Senior Reactor Operator (SRO) responded to the sewage ejector control panel which did not have any lights indicating sewage ejector pump operation. The sewage water had filled the electrical conduit supplying power to the ejector pumps and was trickling out of the sewage ejector control panel, running down the building w~ll. and into a floor drain. The SRO reset the sewage ejector control panel and the pumps restarted. On 10/27/2012 at 23:30 EDT all sewer water leakage was isolated from the control panel. The total estimated quantity of sewer water which reached the floor drain was estimated at less than 1 gallon. The floor drain flows into the turbine building sump. The turbine building sump is discharged to the Low Volume Waste Treatment Pond which discharges through Internal Monitoring Point 103 to the Diffuser Pond. The Diffuser Pond discharges through Outfall 101 to the Tennessee River. At no time was there a threat to public drinking supplies, to human health, or the environment. Period of Discharge The shift manager was notified of the high level alarm and a small amount of water flowing out of the sewage ejector pump control box on 10/27/2012 at 23:00 EDT. The sewer water was running out of the electrical control panel, traveling down the building wall, and entering the floor drain ~elow. The sewage ejector control box was reset and the pumps restarted. The sewer water flowing out of the control panel was stopped on 10/27/2012 at 23:30 EDT. The estimated time of discharge is approximately 30 minutes. Steps Being Taken to Reduce, Eliminate, and Prevent Recurrence This incident was entered into the TVA Corrective Action Program. An investigation is ongoing to determine the exact cause of the loss of power at the turbine building sewage ejector control panel. Sequoyah has implemented. shiftly monitoring of this sewage ejector location as an added precaution until the apparent cause has been identified and repaired to prevent another overflow. An update on the apparent cause will be provided in the November DMR submittal.
TVA Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 3 The turbine building sump was discharged directly to the yard drainage pond on 10/24/2012 and 10/28/2012- 10/31/2012. During this period, the turbine building sump was monitored in accordance with the narrative condition found in Part 1.A.2 of NPDES Permit TN0026450. Turbine Building Sump Monitoring Data Parameter Daily Minimum Monthly Average Daily Maximum No. of Samples Flow - 1.525 MGD 2.580 MGD 5 pH 8 s.u. - 9 s.u. 5 O&G - < 5 mg/L < 6 mg/L 5 TSS - 7 mg/L 10 mg/L 5
PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. Na~-~A-SEQU~AANUCL~RP~NT~-- DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 _Mdres!,_ _f.Q,_BOX....2.POO - - - - - - - - - - _ _ F- FINAL ---~TEROFFICEOPS-5N-S~-------- - _ _ _§ODDY- ________ _ DAISY..I.N~7384 DIFFUSER DISCHARGE Fa~-~A-SEQU~HNUCL~RP~N~---- EFFLUENT g~i~~MIITONCOUN~---------- A TIN: Brad Love
*** NO DISCHARGE D ***
NOTE: Read instructions before completina this fonn X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 36.0 0 31/31 RCORDR CENTIGRADE M~SUREMENT ** 04 00010 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
******** ******** - ******** ******** Req *. Mon.
DAILY MAX DEG.C. CONTI NUOUS CALCTO TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 25.4 0 31/31 MODELD M~SUREMENT ** 04 CENTIGRADE 00010 z 0 PERMIT. ******** ******** ******** 30.5 DEG.C. CONTI CALCTD REQUIREMENT INSTREAM MONITORING DAILYMX* NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** 3 0 31/31 CALCTD MEASUREMENT ** 04 UPSTRM DEG.C 00016 1 s PERMIT ******** ******** ******** ******** 3 DEG.C. CONTI CALCTD REQUIREMENT EFFLUENT GROSS DAILYMX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** 1755 ******** ******** ******** 0 31/*31 RCORDR M~SUREMENT 03 ** TR~TMENT P~NT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** **~**** ******** CONTI RCORDR REQUIREMENT EFFLUENT GROSS DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** 0.015 0.059 0 23/31 GRAB M~SUREMENT ** 19 50060 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
******** ******** - ******** 0.1 MOAVG 0.1 DAILY MAX MGIL FIVE PER CALCTD WEEK TEMPERATURE* C, RATE OF SAMPLE ******** 0 ******** ******** 0 31/31 CALCTD M~SUREMENT 62 **
CHANGE 82234 1 0 PERMIT REQUIREMENT
******** 2 DEG * ******** ******** ******* CONTI CALCTD EFFLUENT GROSS C/HR NUOUS DAILYMX SAMPLE M~SUREMENT PERMIT REQUIREMENT II t?!~-
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I. Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Paul R. Simmons properly gather and evaluate the information submitted. Based on my inquiry of the person or ~ persons who manage tile system, or those persons directly responsible for gathering the Information, tho information submitted is , to the best of my knowledge and belief, true, a=rate, 423 843-6502 12 11 10 Sequoyah Plant Manager and complete. I am aware that there ara significant penalties for submitting falsa information, SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: 1. Floguard MS6236 (max. calc. cone. was 0.029mg/L-Iimit 0.2mg/L) 2. Biodetergent 73551 (max .. calc. cone. was 0.038mg/L-Iimit 2."0mg/L) 3. Spectrus CT1300 (max. calc. cone. was 0.036mg/L-Iimit 0.050mg/L) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
Mean# of NOTES:%
-Mean# of Water Water Sample Date %Settlers Sample Date Asiatic LOCATION SUB LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp.("C) Temp.fC)
Clams/m3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW W8E 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 P8 01/24/2012 o* 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 88 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5. 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 W8E 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 W8E 03113/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 W8E 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 W8E 03/27/2012 1623 . 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 W8E 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 P8 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 P8 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May2012 No Samples Collected June 2012 No Samples Collected July 2012 No Samples Collected August 2012 No Samples Collected September 2012 No Samples Collected 10/23/2012 394 8 17 10/23/2012 82 17 1-ISV-24-1234 WAW
PERMilTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. Na~-~A-SEQUO~HNUCL~RPUNT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 F- FINAL
= = .J!..NTEROFFICE OPS-5N-SQID=- = = = = =
---~D~-MI~J~73~-------- BIOMONITORING FOR OUTFALL 101 Fa~-~A-SEQU~HNUCL~RP~NC ____ _ EFFLUENT L~~~~I~NCOUN~---------- ATTN: Brad Love
*** NO DISCHARGE D ***
NOTE: Read instructions before completin!l this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM. AVERAGE MAXIMUM UNITS
~PLE Monitoring IC25 STATRE 7DAY CHR M~SUREMENT
******** ******** ** ******** ******** 23 CERIODAPHNIA Not Required TRP3B 1 0 PERMIT ******** ******** 43.2 ********" ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MINIMUM ANNUAL S~PLE Monitoring IC25 STATRE 7DAY CHR ******** ******** ** ******** ******** 23 M~SUREMENT
- Not Required PIMEPHALES TRP6C 1 0 PERMIT ******** ******** 43.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MIMINUM ANNUAL SAMPLE M~SUREMENT PERMIT REQUIREMENT .
SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT S~PLE M~SUREMENT PERMIT REQUIREMENT S~PLE M~SUREMENT PERMIT REQUIREMENT
~
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of Jaw that this document and ell attechments were prepared under my TELEPHONE DATE diredion or supervision In acccirdance with a system designed to assure that qualified personnel Paul R. Simmons properly gather and evaluate the information.submilled. Based on my inquiry of the person or persons who manage the system, or those persons diredly responsible for gathering the Plant Manager Information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-6502 12 11 10 Sequoyah Plant Manager and complete. I am awere that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of line and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) Toxicity was not sampled in October 2012. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Fonn Approved. Name_~A-SEQUO~~UCL~RP~NT _ _ _ _ DISCHARGE MONITORING REPORT" (DMR) (SUBR 01) OMB No. 2040-0004 Addres!,__E.O. BOX2000 - - - - - - - - - - - -
---~TEROFFICEOPS-5N-S~-------- TN0026450 F -FINAL ---~D~-MI~J~738L _______ _ PERMIT NUMBER LOW VOL. WASTE TREATMENT POND Fa~-~A-S~~HNUCL~RP~N_ _ _ _ _ _
EFFLUENT L~~~MI~NCOUN~---------- A TIN: Brad Love
*** NO DISCHARGE D ***
NOTE: Read instructions before complelln!l this fonn X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH SAMPLE M~SUREMENT
- 7 ********
8 12 0 19/31 GRAB 00400 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT - MINIMUM 6 9 MAXIMUM su THREE/ WEEK GRAB
- SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** 9 2/31 GRAB M~SUREMENT
******** ** 13 19 0 00530 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT --*--*-- ******** **
*---**** 30 MOAVG 100 DAILYMX MGIL TWICE/
MONTH GRAB OIL AND GR~SE SAMPLE ******** ********
- ********* <6 <6 19 0 2/31 GRAB M~SUREMENT 00556 1 0 PERMIT REQUIREMENT
---*** 15 20 MGIL TWICE/ GRAB EFFLUENT GROSS MOAVG DAILYMX* MONTH FLOW, IN CONDUIT OR THRU TR~TMENT PLANT SAMPLE M~SUREMENT 1.242 1.834 03 ******** ********
........ . - 0 31/31 RCORDR 50050 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT Req *. Mon.
MOAVG Req. Mon DAILYMX MGD
*----*** *----*-- SEE PERMIT RCORDR
- SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT
~~.
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared undar my TELEPHONE; DATE direction or supervision in accordance with a system designed to assure that qualified personnel Paul R. Simmons property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manege the system, or those persons directly responsible for gathering the Sequoyah Plant Manager Information, the information submitted is , to the best of my knowiedge and belief, true, accurate, 423 I 843-6502 12 11 10 and complate. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED including the possibility of line and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
NATIONAL POll lJTANT DISCHARGE EUMINATION SYSTEM (NPDESJ MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMRI (SUBR 01) OMB No. 20-40-0004 TN0026450 110 G F- FINAL PERMIT NUMBER RECYCLED COOLING WATER EFFLUENT ATTN: Brad love
.... NO DISCHARGE IXX I ....
NOT E: Read instructions before completing lhi s form PARAMETER QUANllTY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE 1--A-VE -=RA~G"'"E---r-- MAXI
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!TEMPERATURE, WATER DEG. SAMPLE MEASUREMENT 04 CENTIGRADE 0!)010 1 0 PERMIT REQUIREMENT
**'****** ........... ............ ......... REPORT DEGC CONTIN CALCTD EFFLUENT GROSS VALUE DAILYMX uous TEMPERATURE, WATER DEG.
CENTIGRADE 00010 z 0 SAMPLE MEASUREMENT PERMIT
******** 30.5 04 OEGC CONTIN CALCTD
-- -- uous REQUIREMENT INSTREAM MONITORING
!TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C SAMPLE MEASUREMENT
- DAILYMX 04 00016 1 0 PERMIT ****'**** 5 OEGC CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT uous FLOW, IN CONDUIT OR THRU SAMPLE ******'** 03
........... DAILYMX TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. MGD ******** CONTIN RCORDR EFFLUENT GROSS VALUE REQUIREMENT DAILY MX uous CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 19 50060 0 EFFLUENT GROSS VALUE PERMIT REQUIREMENT
......... ******** 0.1 0.1 MG/L Frve per Week CALCTD MOAVG DAILYMX TEMPERATURE- C, RATE OF SAMPLE 04 *******'*
CHANGE MEASUREMENT 82 234 0 PERMIT 2 DEGC .............. CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE DAILYMX uous SAMPLE MEASUREMENT PERMIT REQUIREMENT
. /7 NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify tRier penalty of law lhallhis document and all attachments were prepared under my ~~- TELEPHONE DATE cf.-edicn or supeMsion in acccrt1ance wllh a syslem dasigned to assure thai qualified personnel Paul R. Simmons property galher and evaluate the intormalion submitted. Based en my inquiry clthe person ot (
persons wno manage the system. or lhose persons diredly responsible for gathenng the S quoyah Plant Manager lr1klrmabcn, the llllotma~cn submrtled is , to lhe best cl my knowledge and belief, true, ac::aKale. l--::-:,.-,-,-:::--::=-:~::-:::-::-:-:-,.,.,::-:-:--==::-==-=::---l 423 I 843-6502 12 11 10 Sequoyah Plant Manager anct complete_1 am ewanolhat 1t1ere are significanl pena!lles tor submitting false infonnatJon, SIGNATURE OF PRINCIPAL EXECUTIVE 1 - - - - - = = - - - - - - - - - - - - lincluding the possibility cl fine and imprisorvnent lor knowvlg YIOiabons. TYPED OR PRINTED OFFICER OR ALITHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AN D EXPLANATION OF ANY VIOLATIONS (Reference all attachments here} No Discharge this Period EPA Form 3320*1 (REV 3/!r.l) Previous edition s may be *used Page 1 of 1
PERMmEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Na~-~A-SEQUO~HNUCL~RP~NT ___ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 204()-()004 (SUBR 01) Addres.L _e.Q._BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ .J!.NTEROFFICE OPS-5N-SQID _ _ _ _ _ _ _ _ TN0026450 F- FINAL ---~DDY-MISU~73~----~-~- PERMIT NUMBER RECYCLED COOLING WATER Fa~-~A-SEQU~HNUCL~RP~NC _ _ _ _ _ EFFLUENT L~~~MI~NCOUN~---------- ATTN: Brad Love
*** NO DISCHARGE IXX I ***
NOTE: Read instructions before completinQ this fonn PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ******** ******'**
'******** ******** 23 CERIODAPHNIA TRP3B 1 0 EFFLUENT GROSS VALUE 0
M~SUREMENT PERMIT REQUIREMENT
******** 43.2 ******** ........ PERCENT SEMI COMPOS MINIMUM ANNUAL IC25 STATRE 7DAY CHR PIMEPHALES SAMPLE M~SUREMENT
- ******** ******** 23 TRP6C 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT
******** ******** - 43.2 .
MINIMUM
**"**** *****"* PERCENT SEMI ANNUAL COMPOS SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT
- SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT.
SAMPLE MEASUREMENT PERMIT. REQUIREMENT
......-=-
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of Jaw that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified personnel Paul R. Simmons properly gather and evaluate the information submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Information, the Information submitted is , to the bast of my knowledge and belief, true, accurate, 423 843-6502 12 11 10 Sequoyah Plant Manager and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} MAJOR Fonn Approved.
============
Name TVA- SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR} (SUBR 01) OMB No. 2040-0004 Address P.O. BOX2000 TN0026450 118 G F -FINAL ---~mEROFFICEOPS~N~~-------- ---~D~-MI~J~73~-------- WASTEWATER & STORM WATER Fa~-~A-SE~~HNUCL~RP~NC ____ _ EFFLUENT ~~~~~I~NCOUN~---------- ATTN: Brad Love
*** NO DISCHARGE IXX I ***
NOTE: Read Instructions before complellnR this fonn X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENcy SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS
~PLE OXYGEN, DISSOLVED (DO) ******** ******** ******** ********
00300 1 EFFLUENT GROSS 0 M~SUREMENT PERMIT REQUIREMENT MINIMUM 2 ........ -- 19 MGIL TWICE/ WEEK GRAB S~PLE SOLIDS, TOTAL SUSPENDED ******** ******** "* ******** ******** 19 M~SUREMENT 00530 1 EFFLUENT GROSS 0 SOLIDS, SETTLEABLE PERMIT REQUIREMENT S~PLE 100 DAILYMX MGIL TWICE/ WEEK GRAB
"* 25 00545 1 0 M~SUREMENT PERMIT REQUIREMENT
........ ******** - ******** ........ 1 MUL ONCE/ GRAB EFFLUENT GROSS DAILYMX MONTH FLOW, IN CONDUIT OR THRU S~PLE ******** ********
03 ******** "* TREATMENT PLANT 50050 1 0 M~SUREMENT PERMIT REQUIREMENT Req. Mon. Req. Mon. MGD ******** ----- ........
- ONCE/ ESTIMA EFFLUENT GROSS MOAVG DAILYMX BATCH
~PLE M~SUREMENT PERMIT REQUIREMENT S~PLE M~SUREMENT PERMIT REQUIREMENT S~PLE M~SUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER. I Certify under penally of law that this document and all attachments were prepared under my
~~nager TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Paul R. Simmons properly gather and evaluate the information submitted. Based on my Inquiry of the person or parsons who manage the system, or those parsons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate. 423 843-6502 12 11 10 Sequoyah Plant Manager and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREAl NUMBER Y~R MO DAY TYPED OR PRimED CODE COMMENTS AND EXPLANATION OF ANY VIO~TIONS (Reference all attachments here)
During this reporting period, there has been no fiQw from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- October 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 11/09/2012 CONCURRENCES Name R c Signature - Comment Date v N
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B.M. Love X A\.._ ---, L.M. Koby X ICL----'
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*,,.) I J.A. Cross X '/c'AU.t., 01'* I rJ_-t.:..-:?.~ illn*/1 d-INSTRUCTIONS: Originator will determine the review/concurrence assignment.
REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpo$e, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.
S58 121214 800- NPDES CORRESPONDENCE December 14, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- DISCHARGE MONITORING REPORT (DMR) FOR NOVEM BER 2012 Enclosed is the November 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The turbine building sump (TBS) discharged directly to the yard drainage pond during the reporting period and was monitored in accordance with the narrative condition found in Part 1.A.2. of the subject permit. Also enclosed are two attachments containing information regarding a collection system overflow that occurred during the reporting period , TBS monitoring data, and an update for previously reported collection system overflows. At no time has there been any observed threat to public drinking supplies, to human health, or the environment. If you have any questions or need additional information , please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D. Booker, MOB 1F-WBN J.W . Proffitt, OPS 4C-SQN B. E. Brickhouse, BR 4A-C A. A. Ray, WT 11A-K J. T. Carlin, OPS 4A-SQN G. R. Signer, WT 6A-K J. A. Cross, POB 2A-SQN P.R. Simmons, POB 2B-SQN T.R. Markum , BR 4A-C B. N. Smith (EDMS), MPB 1E-M D. B. Nida, BR 4A-C
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 December 14, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- DISCHARGE MONITORING REPORT (DMR) FOR NOVEMBER 2012 Enclosed is the November 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The turbine building sump (TBS) discharged directly to the yard drainage pond during the reporting period and was monitored in accordance with the narrative condition found in Part 1.A.2. of the subject permit. Also enclosed are two attachments containing information regarding a collection system overflow that occurred during the reporting period, TBS monitoring data, and an update for previously reported collection system overflows. At no time has there been any observed threat to public drinking supplies, to human health, or the environment. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
.'*Carlin
§" e 1ce President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013
TVA Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 1 Description of the Event and Determination of Cause On 11/25/2012 at 22:13 EDT the Sequoyah (SQN) Shift Manager was notified of a low-flow sewage overflow coming from a manhole located in the roadway between the Plant Office Building, Multi-Purpose Building, and Security Building. The shift manager initiated the SQN Spill Plan and dispatched personnel to investigate. An intermittent stream of sewage was discovered flowing out of the manhole and running approximately 20 feet across the road before it reached a yard drain. On 11/25/2012 at 22:30 EDT the sewage overflow was stopped from entering the yard drain. All remaining sewage residue in the road was neutralized with lime. The yard drain flows to the SQN Yard Drainage Pond, which discharges to the Diffuser Pond. The Diffuser Pond discharges through Outfall101 to the Tennessee River. At no time was there any observed threat to the public drinking supplies, to human health, or the environment.
- The total quantity of sewage that overflowed into a yard drain was estimated to be less than 5 gallons. The overflow was caused by a mass of foreign material becoming entangled around the ejector pump float switch. The ejector pump float switch became locked in the high level position and the pump continued to run. The ejector pump eventually tripped du.e to overheating which led to an associated trip of. the high level alarm.
Period of Discharge The shift manager was notified of the overflow on 11/25/2012 at 22:13 EDT. The sewage overflow was entering the yard drain as personnel responding to the spill arrived. The sewage was stopped from entering the yard drain on 11/25/2012 at 22:30 EDT. The estimated time of discharge was approximately 17 minutes. Steps Being Taken to Reduce, Eliminate, and Prevent Recurrence This incident was entered into the TVA Corrective Action Program. Sequoyah continues daily monitoring of sewage ejector pump locations to ensure control power has not been isolated and has added visual inspections of associated manholes to verify the absence of foreign material which may cause ejector pump issues.
TVA Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 2 Turbine Building Sump Monitoring Data The turbine building sump was discharged directly to the yard drainage pond from 11/10/2012 to 11/15/2012. During this period, the turbine building sump was monitored in accordance with the narrative condition found in Part 1.A.2 of NPDES Permit TN0026450. Parameter Daily Minimum Monthly Average Daily Maximum No. of Samples Flow - 0.480 MGD 1.150 MDG e pH 8 s.u. - 8 s.u. 5 O&G - < 4 mg/L < 6 mg/L 5 TSS - 4 mg/L 7 mg/L 5 Update on Previously Reported Sewage Collection System Overflows SQN is herein providing an update regarding sewage collection system overflow events previously reported to the Division. Each event was entered into the TVA Corrective Action Program, and an investigation was initiated to determine the cause(s). Throughout the investigation and the duration of the Steam Generator Replacement outage, Sequoyah has implemented shiftly monitoring of onsite sewage ejectors as an added precaution. SQN determined the sewage collection system overflows had two apparent causes. The . investigation. indicated there was no consistent method to inform secondary and tertiary electrical-feeds when switching circuit breakers of primary electrical systems. The investigation also indicated there are potential opportunities to improve the inspection program. As a result, TVA will elicit the involvement of a Grade I collection system operator to review the existing system and provide recommendations for necessary upgrades to the material condition and inspection program. The review will include at a minimum an evaluation of current aiarm system design and inspection program scope and frequency. In addition, measures will be taken by site personnel to improve response awareness and ensure prompt _and appropriate actions continue to be taken.
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Nar:rut _ _!_VA- SEQUOYAH NUCLEAR PLANT _ _ _ _ . DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMS No. 2040-0004 AddreS.L ....f.Q._BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICEOPS-5N-S~-------- TN0026450 F- FINAL ---~D~-MI~~~73~-------- DIFFUSER DISCHARGE Fa~-~A-SEQU~HNUCL~RP~NC ____ _ EFFLUENT ~ti~~MI~NCOUN~----------
*** NO DISCHARGE ATTN: Brad Love NOTE: Read instructions before completina this form X
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. CENTIGRADE SAMPLE M~SUREMENT
- ******** ******** 29.6 04 0 30/30 RCORDR 00010 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
- -~****** ******** Req. Mon.
DAILY MAX DEG.C. CONTI NUOUS CALCTD TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 17.4 0 30/30 . MODELD M~SUREMENT ** 04 CENTIGRADE 00010 z 0 PERMIT ******** ******** ******** ******** 30.5 DEG.C. CONTI CALCTD REQUIREMENT INSTREAM MONITORING DAILYMX NUOUS* TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C SAMPLE M~SUREMENT
- ******** ******** 2 04 0 30/30 CALCTD 00016 1 EFFLUENT GROSS 1 PERMIT REQUIREMENT DAILYMX 5 DEG.C. CONTI NUOUS CALCTO FLOW, IN CONDUIT OR THRli SAMPLE ******** 983 ******** ******** ******** 0 30/30 RCORDR 03 **
TREATMENT PLANT 50050 1 0 M~SUREMENT PERMIT REQUIREMENT
*****"** Req. Mon. MGD ******** ........ ******** .... CONTI NUOUS RCORDR EFFLUENT GROSS DAILY MAX CHLORINE, TOTAL RESIDUAL 1
SAMPLE
. M~SUREMENT PERMIT
........ - - ******** 0.012 0.1 0.020 19 MG/L 0 14/30 FIVE PER CALCTD GRAB 50060 0 REQUIREMENT
'******** 0.1 EFFLUENT GROSS MOAVG DAILY MAX WEEK TEMPERATURE- C, RATE OF CHANGE SAMPLE M~SUREMENT
******** 0 62 ******** ********
0 30/30 CALCTD 82234 1 0 PERMIT ******** 2 DEG ******** ******** ******* CONTI CALCTD REQUIREMENT C/HR EFFLUENT GROSS DAILYMX NUOUS SAMPLE M~SUREMENT PERMIT REQUIREMENT
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~~-
NAMEITIT.LE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure the! qualified parsonnel( John T. Carlin proparly gather and evaluate the infonnalion submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the
. Vtl:e" ~!'resident 423 infonnalion, the*lnfonnation submitted is , to the best of my knowledge and belief, true, accurate, 843-7001 12 12 14 Site Vice President and complete. I am aware that there ere significant panallies for submitting false infonnation,
~~*OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. ER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here)
No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: 1. Floguard MS6236 (max. calc cone. was 0.1 03mg/L-Iimit 0.2mg/L), 2. Biodetergent 73551 (max. calc. cone. was 0.051 mg/L-Iimit 2.0mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
Mean# of NOTES:% Mean# of Water Water Sample Date %Settlers Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp. ("C) Temp. ("C) Clams/m3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 . 0 10 PB 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE 03/13/2012 0 0 13 03/13/2012 o* 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 PB 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 PB 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May 2012 No Samples Collected June 2012 No Samples Collected July 2012 No Samples Collected August2012 No Samples Collected September 2012 No Samples Collected 10/23/2012 394 8 17 10/23/2012 82 17 1-ISV-24-1234 WAW 10/30/2012 34 50 17 10/30/2012 17 17 1-ISV-24-1234 WAW 11/06/2012 0 0 17 11/06/2012 0 17 1-ISV-24-1234 WAW 11/13/2012 0 0 15 11/13/2012 0 15 1-ISV-24-1234 WAW 11/20/2012 0 0 13 11/20/2012 0 13 1-ISV-24-1234 WAW 11/28/2012 0 0 12 11/28/2012 0 ~2 1-ISV-24-1234 WAW 12/04/2012 0 0 12 12/04/2012 0 12 1-ISV-24-1234 WAW
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Fonn Approved. Name_~A-SEQUO~~UCLEARP~NT~-- DISCHARGE MONITORING REPORT (DMRJ (SUBR01) OMB No. 2040-0004 Addres!.__E.O. BOX2000 - - - - - - - - - - - - F -FINAL ---~TEROFFICEOPS-5N-S~---~---- ---~D~-MI~~~73BL _ _ _ _ _ _ _ _ BIOMONITORING FOR OUTFALL 101 Fa~-~A-SEQU~HNUCL~RP~NC _ _ _ _ _ EFFLUENT L~~~MIUONCOUN~--------~- ATTN: Brad Love
*** NO DISCHARGE D ***
NOTE* Read instructions before completin!l this fonn X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRAnON NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE M~SUREMENT
******** ******** ... Monitoring ******** ***'***** 23 CERIODAPHNIA Not Required TRP3B 1 0 PERMIT ******** ******** 43.2 ******** *******<<! PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE M~SUREMENT
******** ******** ... Monitoring ******** ******** 23 PIMEPHALES Not Required TRP6C
- 1 0 PERMIT ******** ******** 43.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MIMINUM ANNUAL SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT
/
REQUIREMENT ( ---~n ./J .**
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of Jaw that this document and all allachmants were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel I John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the 'L-;... information, the information submitted is ,*to the bast of my knowledge and belief, true, accurate, -- *- T restdent 423 843-7001 12 12 14 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, Slef[ATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisoAment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) Toxicity was not sampled in November 2012. EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} MAJOR Form Approved. Name_~A-SEQUO~HNUCL~RPUNT ___ _ DISCHARGE MONITORING REPORT (DMR} (SUBR 01) OMB No. 2040-0004 Addres.!_ ...f.O. BOX 2000 _ _ _ _ _ --' _ _ _ _ _ _ ---~TE~FFICEOPS-5N-S~-------- F- FINAL ---~D~-MISU~73~-------- LOW VOL. WASTE TREATMENT POND Fa~-~A-SEQUO~HNUCL~RP~NC _ _ _ _ _ EFFLUENT ~cati~~MIUONCOUN~---------- A TIN: Brad Love
*** NO DISCHARGE D ***
NOTE: Read instructions before completin!l this form l>< PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY S~MPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH 00400 1 0 SAMPLE M~SUREMENT PERMIT
***~****
........ - - 7 6
8 12 su 0 13/30 THREE/ GRAB REQUIREMENT 9 GRAB EFFLUENT GROSS MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE M~SUREMENT
- ******** 8 10 19 0 2/30 GRAB 00530 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
******** ******** - ******** 30 MOAVG 100 DAILYMX MGIL lWICEI MONTH GRAB OIL AND GREASE SAMPLE M~SUREMENT
- ******** <6 <6 19 0 2/30 GRAB 00556 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
******** ******** - ******** 15 MOAVG 20 DAILYMX MGIL lWICEI MONTH GRAB FLOW, IN CONDUIT OR THRU TR~TMENT PLANT 50050 1 0 SAMPLE M~SUREMENT PERMIT 1.254 Req. Mon.
1.685 03 MGD
-.. 0 30/30 RCORDR Req.Mon SEE RCORDR REQUIREMENT EFFLUENT GROSS MOAVG DAILYMX PERMIT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my
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'r1ffP TELEPHONE DATE direction or supervision in accordance with. a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for galharing tha
/ **~res1den information, tha information submitted is , to tha bast of my knowiedga and ballet, true, accurate, 423 843-7001 12 12 14 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIG~F PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~-~A-SEQUO~~UCL~RP~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Addres.!__E..O. BOX2000 - - - - - - - - - - - - ---~TEROFFICEOPS-5N-S~-------- TN0026450 110 G F- FINAL ---~D~-M1~~~73~-------- RECYCLED COOLING WATER Fa~-~A-SEQUO~NUCL~RP~NC ____ _ EFFLUENT ~~~~~MIUONCOUN~---------- A TIN: Brad Love
*** NO DISCHARGE IXX I ***
NOTE* Read instructions before completin!l this form PARAMETER TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0
>< SAMPLE M~SUREMENT PERMIT AVERAGE QUANTITY OR LOADING MAXIMUM UNITS MINIMUM QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS 04 DEGC NO.
EX FREQUENCY SAMPLE OF ANALYSIS TYPE CALCTO REQUIREMENT REPORT CONTIN EFFLUENT GROSS VALUE DAILYMX uous TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** M~SUREMENT
******** ** 04 CENTIGRADE 00010 z 0 PERMIT ******** ******** ** ******** ******** 30.5 DEGC CONTIN CALCTO REQUIREMENT INSTREAM MONITORING DAILYMX uous TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ********
M~SUREMENT
******** ** 04 UPSTRM DEG.C 00016 1 0 PERMIT ******** ******** ** ******** ******** 5 DEGC CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE DAILYMX uous FLOW, IN CONDUIT OR THRU SAMPLE ******** ********
M~SUREMENT
******** 03 ******** **
TREATMENT P~NT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ******** ******** "* CONTIN RCORDR REQUIREMENT EFFLUENT GROSS VALUE DAILYMX uous CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** M~SUREMENT ** 19 50060 1 0 PERMIT REQUIREMENT
.......... ******** ** ******** 0.1 0.1 MGIL Five per CALCTD EFFLUENT GROSS VALUE MOAVG DAILYMX Week TEMPERATURE- C, RATE OF SAMPLE ******** 04 ******** ********. ******** **
CHANGE M~SUREMENT 82234 1 0 PERMIT ******** 2 DEGC ******** *****'*** ******** "* CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE DAILYMX uous SAMPLE M~SUREMENT PERMIT
. REQUIREMENT I
/' ---;;;;-;_
I'A .........
~em NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law thetthis dowment and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with e system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Site Vice President information, the information submitted is , to the best of my knowiedga and belief, true, accurate, 423 843-7001 12 12 14 and complete. I am aware that there are significant penalties for submitting false Information, SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine end imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Fonn Approved. Name_~A"SEQUO~~UCL~RP~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 Addres,L...f..Q....BOX2000 - - - - - - - - - * - - - TN0026450 F- FINAL ---~TEROFFICEOPS-5N-S~---~---- ---~D~-MISQ~738L _______ _ RECYCLED COOLING WATER Fa~-~A-SEQUO~HNUCL~RP~NC _ _ _ _ _ EFFLUENT ~cati~~~I~NCOUN~---------- A TIN: Brad Love
*** NO DISCHARGE IXX I ***
NOTE: Read Instructions before completin!l this fonn X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MIN.IMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR S~PLE '*:******* ******** ******** M~SUREMENT ** ******** 23 CERIODAPHNIA TRP3B 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT
******** ******** - 43.2 MINIMUM
******** ******** PERCENT SEMI ANNUAL COMPOS S~PLE IC25 STATRE 7DAY CHR ******** ******** ** ******** ******** 23 M~SUREMENT PIMEPHALES TRP6C 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT
******** ******** - *43.2 MINIMUM
********* ******** PERCENT SEMI ANNUAL COMPOS S~PLE M~SUREMENT PERMIT REQUIREMENT S~PLE M~SUREMENT PERMIT REQUIREMENT S~PLE M~SUREMENT PERMIT REQUIREMENT S~PLE M~SUREMENT PERMIT REQUIREMENT S~PLE M~SUREMENT PERMIT REQUIREMENT /r-,t .
( _%/J ,.--)
~_y NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of Jaw that this document and all attachments were prepared under my TELEPHONE DATE direction or*supervision in eccordance with a syatem designed to assure that qualified personn(
John T. Carlin property gather and e.valuate the information submitted. Based on my inquiry of the person or Site Vice President persons Who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowiedge and belief, true, accurate, 1\:: / ...site Vice President 423 843-7001 12 12 14 and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) MAJOR Form Appro~ed. Name_~A-SEQUO~HNUCLEARPUNT ___ _ DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 AddreS,lL ~.Q....BOX 2000 _ - - - - - - _ _ _ _ _ ---~TEROFFICEOPS-5N-S~-------- TN0026450 118 G F- FINAL ---~D~-MI~~~73BL _______ _ PERMIT NUMBER WASTEWATER & STORM WATER Fa~-~A-SEQU~HNUCL~RP~NL _ _ _ _ _ EFFLUENT ~~~~~IITONCOUN~---------- A TIN: Brad Love
*** NO DISCHARGE IXX I ***
NOTE: Read instructions before complelin!l this form PARAMETER OXYGEN, DISSOLVED (DO)
>< S~PLE M~SUREMENT AVERAGE QUANTITY OR LOADING MAXIMUM UNITS MINIMUM QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS 19 NO. FREQUENCY SAMPLE EX OF ANALYSIS TYPE 00300 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
******** ******** - MINIMUM 2 ********
******** MG/l TWICE/
WEEK GRAB SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** ******** M~SUREMENT ** 19 00530 1 0 PERMIT REQUIREMENT
******** ******** - ******** ******** 100 DAILYMX MG/L TWICE/
WEEK GRAB EFFLUENT GROSS SOLIDS, SETTLEABLE S~PLE ******** ******** ******** M~SUREMENT
******** 25 00545 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
******** ******** - ******** ******** 1 DAILYMX MUL ONCE/
MONTH GRAB FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** TREATMENT PLANT M~SUREMENT 03 ** 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ******** ******** ********
- ONCE/ ESTIMA REQUIREMENT EFFLUENT GROSS MOAVG DAILYMX BATCH S~PLE M~SUREMENT PERMIT REQUIREMENT S~PLE M~SUREMENT PERMIT REQUIREMENT S~PLE M~SUREMENT PERMIT REQUIREMENT r- ~
~ W£/
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that !hill document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the
~te Vice PresiCient '
information, the information submitted is , to lha best of my knowiadge and belief, true, accurate, 423 843-7001 12 12 14 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE L lYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXPLANATION OF ANYVIO~TIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
S58 130107 800- NPDES CORRESPONDENCE January 7, 2013 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- DISCHARGE MONITORING REPORT (DMR) FOR DECEMBER 2012 Enclosed is the December 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. Also enclosed is one attachment containing information regarding a collection system overflow that occurred during the reporting period. At no time has there been any observed threat to public drinking supplies, to human health, or the environment. If you have any questions or need additional information , please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga , Tennessee 37 402-2013 S.D. Booker, MOB 1F-WBN J .W . Proffitt, OPS 4C-SQN B. E. Brickhouse, BR 4A-C A. A. Ray, WT 11A-K J . T. Carlin , OPS 4A-SQN G. R. Signer, WT 6A-K J . A. Cross, POB 2A-SQN P.R. Simmons, POB 2B-SQN T.R. Markum , BR 4A-C B. N . Smith (EDMS), MPB 1E-M D . B. Nida, BR 4A-C
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 January 7, 2013 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA)- SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- DISCHARGE MONITORING REPORT (DMR) FOR DECEMBER 2012 Enclosed is the December 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. Also enclosed is one attachment containing information regarding a collection system overflow that occurred during the reporting period. At no time has there been any observed threat to public drinking supplies, to human health, or the environment. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013
TVA Sequoyah.Nuclear Plant NPDES Permit No. TN0026450 Attachment 1 Description of the Event and Determination of Cause On 12/03/2012 at approximately 19:00 EDT a toilet located in the Mechanical Maintenance shop was observed to be overflowing. The shift manager initiated the SQN Spill Plan and dispatched personnel to investigate. An intermittent stream of sewage was observed overflowing out of the toilet and running approximately 20 feet across the mechanical maintenance shop floor before it entered a floor drain. There were no solids observed in the overflow. On 12/03/2012 at approximately 20:00 EDT the sewage overflow was stopped from entering the floor drain. All remaining sewage residue in the mechanical maintenance shop was neutralized and cleaned up. The floor drain flows to the service building sump. The service building sump is automatically pumped into the SQN Yard Drainage System with discharges to the Yard Drainage Pond. The Yard Drainage Pond discharges to the Diffuser Pond, which discharges through Outfall 101 to the Tennessee River. At no time was there any observed threat to the* public drinking supplies, to human health, or the environment. The overflow was caused by a mass of foreign material becoming lodged in a check valve in the sewage ejector system. The check valve was not able to fully close causing the toilet to overflow as the level in the sewage system increased. Period of Discharge The overflow was observed at approximately 19:00 EDT on 12/03/2012. The sewage overflow was entering the floor drain as personnel responding to the spill arrived. The sewage was stopped from entering the floor drain on 12/03/2012 at approximately 20:00 EDT. The estimated time of discharge was approximately 1 hour. Steps Being Taken to Reduce, Eliminate, and Prevent Recurrence This incident was entered into the TVA Corrective Action Program. Sequoyah has closed the two restrooms in the mechanical maintenance shop until further review of this incident has been conducted. Additionally, plans are being developed to install physical barriers between the restroom and local floor drains that will aide in preventing future overflows from reaching the floor drains.
PERMITTEE NAME/ADDRESS . (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~-~A-SEQUO~~UCL~RPUNT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2o40-0004 (SUBR 01) Md~LX~OX2000_~--------- ---~TEROFFICEOPS-5N-S~-------- TN0026450 101 G F- FINAL ---~D~-MI~~~738L _______ _ DISCHARGE NUMBER DIFFUSER DISCHARGE Fa~-~A-SEQU~HNUCL~RP~NC _ _ _ _ _ ~~~~~~Ml~NCOUN~-~-------- EFFLUENT ATTN: Brad Love
*** NO DISCHARGE D ***
*x NOTE: Read instructions before complelinR this fonn PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM LiN ITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE M~SUREMENT
- ******** ******** 27.6 04 0 31/31 RCORDR CENTIGRADE 00010 1 0 PERMIT ******** ******** ******** DEG.C. CONTI REQUIREMENT
******** Req. Mon. CALCTD EFFLUENT GROSS DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE M~SUREMENT
- ******** ******** 15.4 04 0 31/31 MODELD CENTIGRADE 00010 z 0 PERMIT ******** ******** ******** ******** 30.5 DEG.C. .CONTI CALCTD REQUIREMENI INSTREAM MONITORING DAILYMX NUOUS TEMP. DIFF. BETWEEN SAMP. &
UPSTRM DEG.C SAMPLE M~SUREMENT
******** 3 04 0 31/31
**- 1--*-o==-
CALCTD 00016 1. 1 EFFLUENT GROSS PERMIT REQUIREMENT
******** ******** - ******** ******** 5 DAILYMX DEG.C. CONTI NUOUS CALCTO FLOW, IN CO~DUIT OR THRU TREATMENT PUNT SAMPLE M~SUREMENT
******** 1534 03 ******** ******** ********
- 0 31/31 RCORDR 50050 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
******** Req. Mon.
DAILY MAX MGD ******** ******** ******** - CONTI NUOUS RCORDR CHLORINE, TOTAL RESIDUAL SAMPLE M~SUREMENT
- ******** 0.020 0.037 19 0 14/31 GRAB 50060 1 EFFLUENT GROSS*
0 PERMIT REQUIREMENT
******** ******** - ******** 0.1 MOAVG 0.1 DAILY MAX MGIL FIVE PER CALCTD WEEK TEMPERATURE -C;RATE OF SAMPLE M~SUREMENi
******** 1 62 ******** ********
- 0 31/31 CALCTD CHANGE 82234 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT SAMPLE DAILYMX 2 DEG C/HR
******** ******** ******* - CONTI NUOUS CALCTD M~SUREMENT PERMIT REQUIREMENT
/Tr l /' /l NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 'c""'""'~-**'-*"'"'..._ ""'"' _ _ ,..,..._~ jt 1 rr ~ TELEPHONE DATE
~~Kte JJ~L~r' direction or supervision in accordance with a system designed to assure that qualified person el John T. Carlin properly gather and evaluate the infonnation submiHed. Based on my inquiry of the person o persons who manage the system. or those persons directly responsible for gathering the i President 423 843-7001 13 infonnatron. the infonnation submiHed is , to the best of my knowledge and belief. true, accurst.., 01 07 Site Vice President and complete I am aware that there are srgnificant penalties for submiHing false infonnat1on, SI~RE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here)
No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: 1. Floguard MS6236 (max. calc. cone. was 0.051 mg/L-Iimit 0.2mg/L) 2. Biodetergent 73551 (max. calc. cone. was 0.051mg/L-Iimit 2.0mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
Mean# of NOTES:% Mean# of Water Water Sample Date %Settlers Sample D;ite Asiatic LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp. ("C) Temp. ("C) Ciamslm3 Clam 01/03/2012 14 100 26 01/03/2012 0 26 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW W8E 01/17/2011 0 0 10 01/17/2011 0 10 P8 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 88 02/14/2012 o* 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 W8E 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 W8E 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 W8E 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 W8E 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 W8E 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 P8 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 P8 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May2012 No Samples Collected June 2012 No Samples Collected July 2012 No Samples Collected August 2012 No Samples Collected September 2012 No Samples Collected 10/23/2012 394 8 17 10/23/2012 82 17 1-ISV-24-1234 WAW 10/30/2012 34 50 17 10/30/2012 17 17 1-ISV-24-1234 WAW 11/06/2012 0 0 17 11/06/2012 0 17 1-ISV-24-1234 WAW 11/13/2Q12 0 0 15 11/13/2012 0 15 1-ISV-24-1234 WAW 11/20/2012 0 0 13 11/20/2012 0 13 1-ISV-24-1234 WAW. 11/28/2012 0 0 12 11/28/2012 0 12 1-ISV-24-1234 WAW-12/04/2012 0 0 12 12/04/2012 0 12 1-ISV-24-1234 WAW 12/12/2012 0 0 11 12/12/2012 0 11 1-ISV-24-1234 WAW 12/18/2012 0 0 24.6 12/18/2012 o* 24.6 1-25-545 CR
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUO~~UCLEARPUNT ___ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB lllo. 2040-0004 Addres.l!.__.f.O. BOX2000 - - - - - - - - - - - - ---~TEROFFICEOPS-5N-S~-------- TN0026450 F- FINAL ---~DDY-MISU~738L _______ _ PERMIT NUMBER BIOMONITORING FOR OUTFALL 101 ~~-~A-SEQU~HNUCL~RP~NC _ _ _ _ _ EFFLUENT Lo~ti~~MI@NCOUN~---------- AlTN: Brad Love
*** NO D.ISCHARGE D ***
NOTE: Read instructions before complelinQthis form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM . AVERAGE MAXIMUM* UNITS IC25 STATRE 7DAV CHR SAMPLE M~SUREMENT
- Monitoring ******** ******** 23 CERIODAPHNIA Not Required TRP3B 1 0 PERMIT ******** ******** 43.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MINIMUM ANNUAL IC25 STATRE 7DAV CHR PIMEPHALES SAMPLE M~SUREMENT
- Monitoring ********
******** 23 TRP6C 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
******** ******** - Not Required 43.2 MIMINUM
******** ******** PERCENT SEMI ANNUAL COMPOS SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin
'""""""'W-**o.w*****-.... -_,..,....._:t direction or supervision in accordance with a system designed to assure that qualified person el properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who managa the system. or those persons directly responsible for gathering the information, the information submitted is
- to the best of my knowledge and belief, true. accurate,
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-~ *--~ !! vrce Pre 1dent JA\j 423 TELEPHONE 843-7001 13 DATE 01 07 Site Vice President and complete. I am '!WOre that there. are significant penalties for submitting false information, 51~ OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED Including the possibility of fine and imprisonment for knowing violations. OF R OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was not sampled in December 2012. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUO~HNUCL~R.P~NT ___ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Addres!,_ _f.Q._BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICEOPS-5N-S~-------- 103 G F- FINAL ---~DDY-MI~~~73~-------- LOW VOL. WASTE TREATMENT POND Fa~-~A-SEQUO~HNUCL~RP~NC ____ _ EFFLUENT w~i~~MIITONCOUN~---------- A TIN: Brad Love
*** NO DISCHARGE D ***
NOTE* Read instructions before completinR this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINiMUM AVERAGE MAXIMUM UNITS PH SAMPLE ******** ******** 7 ******** 13/31 M~SUREMENT ** 8 12 0 GRAB 00400 1 0 PERMIT ******** ******** ** 6 ******** 9 su THREE/ GRAB REQUIREMENT EFFLUENT GROSS MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** 12 13 0 2/31 GRAB-- M~SUREMENT ** 19 00530 1 0 PERMIT ******** ******** ** ******** 30 100 MG/l TWICE/ GRAB REQUIREMENT EFFLUENT GROSS MOAVG DAILYMX MONTH OIL AND GREASE SAMPLE ******** ******** ******** <4 <6 0 2/31 -GRAB M~SUREMENT ** 19 00556 1 0 PERMIT ******** ******** ** ******** 15 20 MG/l TWICE/ GRAB REQUIREMENT EFFLUENT GROSS MOAVG DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.205 ******** ******** 31/31 M~SUREMENT 0.963 03 ******** ** 0 RCORDR TREATMENT PLANT 50050 1 0 PERMIT REQUIREMENT Req. Mon. Req. Mon MGD ******** ******** ******** .. SEE RCORDR EFFLUENT GROSS MOAVG DAILYMX PERMIT SAMPLE M~SUREMENT PERMIT REQUIRJ:MENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of Jaw that this document and all attachments wara prepared under my TELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the _,;}He '\1 ce President 423 Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 843-7001 13 01 07 and complete. I am aware that there are significant penalties for submitting false information, SIGNA'TORE OF PRINCIPAL EXECUTIVE I TYPED OR f'RINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Fonn Approved. Name_~A-SEQUO~HNUCL~RPUNT ___ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Addres.!!..._ _E.Q....BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ TN0026450 F- FINAL ---~TEROFFICEOPS-5N-S~-------- Fa~ SODDY
- DAISYJN 37384
~-SEQU~HNUCL~RP~NT Locati~~MI~NCOUN~-~--------
===== PERMIT NUMBER RECYCLED COOLING WATER EFFLUENT ATTN: Brad Love
*** NO DISCHARGE IXX I -*
NOTE: Read instructions before complelin!l this tonn X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY *sAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE M~SUREMENT
- ******** ******** 04 CENTIGRADE 00010 1 0 PERMIT REQUIREMENT
******** ******** - ******** ******** REPORT DEGC CONTIN uous CALCTO
-- DAlLYMX EFFLUENT GROSS VALUE TEMPERATURE, WATER DEG. SAMPLE ****'**** ******** ******** ********
M~SUREMENT 04 CENTIGRADE 00010 z 0 PERMIT ******** ******** ******** ******** 30.5 DEGC CONTIN CALCTO INSTREAM MONITORING REQUIREMENT DAILYMX uous SAMPLE-- TEMP. DIFF. BETWEEN SAMP. & ******** ******** ******** 04 M~SUREMENT UPSTRM DEG.C 00016 1 EFFLUENT GROSS VALUE 0 PERMIT
-REQUIREMENT
******** ******** - ******** ******** 5 DAILYMX DEGC CONTIN uous CALCTO FLOW, IN CONDUIT OR THRU TR~TMENT 50050 1 PUNT 0
SAMPLE M~SUREMENT PERMIT
******** Req. Mon.
03 MGD
-** CONTIN RCORDR REQUIREMENT DAILYMX uous EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** 19 50060 1 0 M~SUREMENT PERMIT REQUIREMENT
******** ........ - ****"*** 0.1 0.1 MG/L Five per Week CALCTD EFFLUENT GROSS VALUE MOAVG DAILYMX TEMPERATURE - C, RATE OF CHANGE SAMPLE M~SUREMENT PERMIT
******** 04 ******** ********
82234 1 0 ******** 2 DEGC ******** CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILYMX uous -- SAMPLE M~SUREMENT PERMIT REQUIREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1CertifY under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or 'Y l persons who manage the system, or those persons directly responsible for gathering the "-i--I.J.--181W\Ifl::8 resident information, the informalion submitted is , to the best of my knowledge and belief, true, accurate, 1--=-='<~'=-:-=~--~:-::-:c-:-:=c-::-:c=-=---1 423 843-7001 13 01 07 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, l-----=-==-==-=-====--------1including the possibility of fine and imprisonment for knowing violations. NUMBER TYPED OR PRINTED AREA Y~R MO DAY CODE COMMENTS AND EXP~NATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} MAJOR Form Approved. Name_~A-SEQUO~HNUCL~RP~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMR} (SUBR 01) OMB No. 2040-0004 Addres,L ...f..Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICEOPS-5N-S~---~~--- TN0026450 F- FINAL ---~DDY-MI~~~73~-------- PERMIT NUMBER RECYCLED COOLING WATER Fa~-~A-SEQUO~HNUCL~RP~NC _ _ _ _ _ EFFLUENT Locati~~MI~NCOUN~---------- A TIN: Brad Love
*** NO DISCHARGE IXX I ***
NOTE* Read instructions before completin!l this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS SAMPLE ******** ******** IC25 STATRE 7DAY CHR M~SUREMENT
******** ** ******** 23 CERIODAPHNIA TRP3B 1 0 0 PERMIT ******** ******** 43.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE
---- MINIMUM ANNUAL SAMPLE ******** ********
IC25 STATRE 7DAY CHR M~SUREMENT
******** ** ******** 23 PIMEPHALES TRP6C 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT
******** ******** - 43.2 MINIMUM
******** ******** PERCENT SEMI ANNUAL COMPOS SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT
*PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my John T. Carlin direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information,.the information submitted is , to the best of my knowiedge and belief, true, accurate, yt~!',f!.t
./ ~'
resident 423 TELEPHONE 843-7001 13
*DATE 01 07 Site Vice President and complete. I am aware that there are significant penalties for submitting false Information, Slur.,,~PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations.
TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved.
============
Name TVA- SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMS No. 2040-0004 Address P.O. BOX2000 118 G F- FINAL ---~TEROFFICEOPS-5N-S~-------- ---~DDY-MISU~738L _______ _ DISCHARGE NUMBER WASTEWATER & STORM WATER Fa~-~A-SEQU~HNUCL~RP~NC _ _ _ _ _ EFFLUENT Lo~i~~MI~NCOUN~----------
*** NO DISCHARGE IXX I ***
A TIN: Brad Love NOTE* Read instructions before completin!l this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO) SAMPLE M~SUREMENT
- ******** ******** 19 00300 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
******** ******** - MINIMUM 2 ******** ******** MG/l TWICE/
WEEK GRAB SOLIDS, TOTAL SUSPENDED SAMPLE M~SUREMENT
- ******** ******** 19 00530 1 0 PERMIT REQUIREMENT
******** ******** - ******** ******** 100 DAILYMX MG/l TWICE/
WEEK GRAB EFFLUENT GROSS *---
---*----*--- f-*-----
SOLIDS, SETTLEABLE SAMPLE ******** ******** ******** ******** M~SUREMENT 25 00545 1 0 PERMIT REQUIREMENT DAILYMX 1 ML/l ONCE/ MONTH GRAB EFFLUENT GROSS FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** M~SUREMENT 03 TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ******** ******** ********
- ONCE/ ESTIMA REQUIREMENT EFFLUENT GROSS MOAVG DAILYMX BATCH SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT SAMPLE M~SUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of Jaw that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the informaiion submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submiHed is , to the best of my knowledge and belief, true, accurate, 1--=Jt-:-:-~~=-=-+/---::::-=:==-::::--:=:-::-:=:-:::---l 423 843-7001 13 01 07 Site Vice President end complete. I am awere that there are significant penalties for submitting false information, 1-------=-:==-==-=--=----::-:-:==--------lincluding the possibility of fine and imprisonment for knowing violations. NUMBER AREA Y~R MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Previous editions niay be used Page 1 of 1
REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- December 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 1/04/2013 CONCURRENCES Name R c Signature - Comment Date v N L2._ ')
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(" B.M. Love X l[ :l v~ \, iJ;v L.M. Koby X "--J J Ill X
~-hk -~ ~-' 'I4 /e'J J.A. Cross* X
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I /;//~ I I INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to* sign the document for the agency.}}