ML060320087

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Annual Lake Norman Maintenance Monitoring Program: 2004 Summary
ML060320087
Person / Time
Site: McGuire, Mcguire  Duke Energy icon.png
Issue date: 01/24/2006
From: Gordon Peterson
Duke Power Co
To:
Document Control Desk, Office of Nuclear Reactor Regulation
References
NC0024392
Download: ML060320087 (148)


Text

I PkDuke GARY R. PETERSON MEPowerv Vice President McGuire Nuclear Station A Duke Energy Company Duke Power MGO1VP / 12 700 Hagers Ferry Road Huntersville, NC 28078-9340 704 875 5333 704 875 4809 fax grpeters@duke-energy. corn January 24, 2006 U. S. Nuclear Regulatory Commission Document Control Desk Washington, D.C. 20555

Subject:

McGuire Nuclear Station Docket Nos. 50-369, 50-370 Please find attached a copy of the annual "Lake Norman Maintenance Monitoring Program: 2004 Summary," as required by the National Pollutant Discharge Elimination System (NPDES) permit NC0024392. The report includes detailed results and data comparable to that of previous years. The report was submitted to the North Carolina Department of Environment and Natural Resources on January 12, 2006.

Questions regarding this submittal should be directed to Kay Crane, McGuire Regulatory Compliance at (704) 875-4306.

Gary R. Peterson

%jI.Ez $

www. duke-energy. corn

U. S. Nuclear Regulatory Commission Document Control Desk January 24, 2006 Page 2 cc: Mr. J. F. Stang, McGuire Project Manager Office of Nuclear Reactor Regulation U.S. Nuclear Regulatory Commission Washington, D.C. 20555 Mr. W. D. Travers, Regional Administrator U.S. Nuclear Regulatory Commission Region II Atlanta Federal Center 61 Forsyth St., SW, Suite 23T85 Atlanta, Georgia 30303 Mr. Joe Brady Senior Resident Inspector McGuire Nuclear Station

LAKE NORMAN MAINTENANCE MONITORING PROGRAM:

2004

SUMMARY

McGuire Nuclear Station: NPDES No. NC0024392 Duke Power A Duke Energy Company December 2005

TABLE OF CONTENTS Page EXECUTIVE

SUMMARY

i LIST OF TABLES vi LIST OF FIGURES vii CHAPTER 1: McGUIRE NUCLEAR STATION OPERATION Introduction 1-1 Operational data for 2004 1-1 CHAPTER 2: WATER CHEMISTRY Introduction 2-1 Methods and Materials 2-1 Results and Discussion 2-3 Future Studies 2-12 Summary 2-12 Literature Cited 2-14 CHAPTER 3: PHYTOPLANKTON Introduction 3-1 Methods and Materials 3-1 Results and Discussion 3-2 Future Studies 3-9 Summary 3-9 Literature Cited 3-10 CHAPTER 4: ZOOPLANKTON Introduction 4-1 Methods and Materials 4-1 Results and Discussion 4-2 Future Studies 4-6 Summary 4-6 Literature Cited 4-8 CHAPTER 5: FISHERIES Introduction 5-1 Methods and Materials 5-1 Results and Discussion 5-3 Future Studies 5-8 Summary 5-8 Literature Cited 5-9

EXECUTIVE

SUMMARY

As required by the National Pollutant Discharge Elimination System (NPDES) permit number NC0024392 for McGuire Nuclear Station (MNS), the following annual report has been prepared. This report summarizes environmental monitoring of Lake Norman conducted during 2004.

McGUIRE NUCLEAR STATION OPERATION The monthly average capacity factor for MNS was 101.3 %, 101.2 %, and 101.9 % during July, August, and September of 2004, respectively (Table 1-1). These are the months when conservation of cool water and discharge temperatures are most critical and the thermal limit for MNS increases from a monthly average of 95.0 'F (35.0 0 C) to 99.0 0 F (37.2 0 C). The average monthly discharge temperature was 97.7 'F (36.5 0C) for July, 97.6 'F (36.4 0 C) for August, and 94.2 'F (34.6 0 C) for September 2004. The volume of cool water in Lake Norman was tracked throughout the year to ensure that an adequate volume was available to comply with both the Nuclear Regulatory Commission Technical Specification requirements and the NPDES discharge water temperature limits.

WATER CHEMISTRY Annual precipitation in the vicinity of MNS in 2004 totaled 44.6 inches or 17.1 inches less than observed in 2003, but similar to the long-term precipitation average for this area (46.3 inches). Air temperatures in 2004 were generally warmer than measured in 2003, as well as the long-term mean. The most pronounced differences occurred in May when 2004 temperatures averaged 3.5 'C warmer than 2003, and 2.6 'C warmer than the long-term average.

Temporal and spatial trends in water temperature and DO in 2004 were similar to those observed historically, and all data were within the range of previously measured values.

Water temperatures in 2004 were generally warmer than observed in 2003 in both the mixing and background zones. Winter temperatures averaged about 0.3 'C warmer throughout the water column in 2004 versus 2003. Summer temperatures averaged about 2 to 3 'C warmer in 2004 versus 2003, with the primary differences observed in the upper 10 m of the water column. Interannual differences in water temperatures, especially in the surface waters, typically paralleled differences exhibited in air temperatures between 2004 and 2003.

i

Reservoir-wide isotherm and isopleth information for 2004, coupled with heat content and hypolimnetic oxygen data, illustrated that Lake Norman exhibited thermal and oxygen dynamics characteristic of historical conditions and similar to other Southeastern reservoirs of comparable size, depth, flow conditions, and trophic status. Availability of suitable pelagic habitat for adult striped bass in Lake Norman in 2004 was generally similar in distribution and amount to historical conditions observed annually since 1983. Despite similarities in habitat conditions to previous years, the largest striped bass die-off ever observed in the reservoir (2599 fish) occurred in the summer of 2004.

All chemical parameters measured in 2004 were within the concentration ranges previously reported for the lake during both preoperational and operational years of MNS.

Concentrations of metals in 2004 were also low, and often below the analytical reporting limits. Manganese and iron concentrations in the surface and bottom waters were generally low in 2004, except during the summer and fall when bottom waters became anoxic, and the release of soluble forms of these metals into the water column was observed. In contrast to previous years, at no time during 2004 did iron concentrations exceed NC's water quality standard (1.0 mg/L). Manganese levels, however, did exceed the State standard (200 ug/L) in the bottom waters throughout the lake in the summer and fall, and are characteristic of historical conditions.

PHYTOPLANKTON Lake Norman continues to support highly variable and diverse phytoplankton communities.

No obvious short term or long term impacts of station operations were observed.

In 2004 lake-wide mean chlorophyll a concentrations were generally in the mesotrophic range with the exception of May, when chlorophyll concentrations averaged in the oligotrophic range. Chlorophyll concentrations during 2004 were generally within the same ranges as those of 2003. Lake Norman continues to be classified as oligo-mesotrophic based on long term, annual mean chlorophyll concentrations. The highest chlorophyll value recorded in 2004, 10.57 ug/L, was well below the NC State Water Quality standard of 40 ug/L.

In most cases, total phytoplankton densities and biovolumes observed in 2004 were higher than those observed during 2003, and standing crops were within ranges established over previous years. Phytoplankton densities and biovolumes during 2004 never exceeded the NC ii

guidelines for algae blooms. Standing crop values in excess of bloom guidelines have been recorded during six previous years of the Program. As in past years, high standing crops were usually observed at up-lake locations; while comparatively low values were noted down-lake.

Seston dry and ash-free weights were more often higher in 2004 than in 2003, and down-lake to up-lake differences were apparent most of the time. The proportions of ash-free dry weights to dry weights in 2004 were higher than those of 2003, indicating an increase in organic composition among 2004 samples.

Secchi depths reflected suspended solids, with shallow depths related to high dry weights.

The lake-wide mean secchi depth in 2004 was slightly higher than in 2003 and was within historical ranges recorded since 1992.

Diversity, or numbers of taxa, of phytoplankton had decreased since 2003, when the total number of individual taxa was the highest yet recorded. The taxononic composition of phytoplankton communities during 2004 was similar to those of many previous years.

Cryptophytes were dominant in February, while diatoms were dominant during May and November. Green algae dominated phytoplankton assemblages during August. Blue-green algae were slightly more abundant during 2004 than during 2003; however, their contribution to total densities rarely exceeded 5 %.

The most abundant alga, on an annual basis, was the cryptophyte Rhodomonas minuta.

Common and abundant diatoms were Fragillaria crotonensis in May and Tabellaria fenestrata in November. The small desmid, Cosmarium asphearosporumvar. strigosum was dominant in August 2004. All of these taxa have been common and abundant throughout the Maintenance Monitoring Program.

The phytoplankton index (Myxophycean) characterized Lake Norman as oligotrophic during 2004, and was slightly higher than the annual index for 2003. Quarterly index values decreased from the highest in February to the lowest in November. Quarterly values did not reflect maximum and minimum chlorophyll concentrations and phytoplankton standing crops. Location index values tended to reflect increases in chlorophyll and phytoplankton standing crops from down-lake to mid-lake.

iii

ZOOPLANKTON Lake Norman continues to support a highly diverse and viable zooplankton community.

Zooplankton densities, as well as seasonal and spatial trends were generally consistent with historical precedent during 2004, and no impacts of plant operations were observed.

Maximum zooplankton densities occurred in May at three locations and in February at two other locations. Minimum zooplankton densities were most often noted in August. As in past years, epilimnetic densities were higher than whole column densities. Mean zooplankton densities tended to be higher among Background Locations than among Mixing Zone Locations during 2004. In the Mixing Zone, a long term trend of increasing year-to-year densities was observed for May. In addition, long term trends showed much higher year-to-year variability at Background Locations than at Mixing Zone Locations.

One hundred and seventeen zooplankton taxa have been recorded from Lake Norman since the Program began in 1987 (Fifty-two were identified during 2004). Four previously unreported taxa (three cladocerans and one rotifer) were identified during 2004.

Overall relative abundance of copepods in 2004 had decreased since 2003, and they were dominant in ten samples collected during August and November. Cladocerans were dominant in only two samples in August, while rotifers were dominant in 70% of all samples.

Overall, the relative abundance of rotifers had increased since 2003, and their relative abundances were somewhat similar to those of 1995. Historically, copepods and rotifers have most often shown annual peaks in May; while cladocerans continued to demonstrate year-to-year variability.

Copepods were dominated by immature forms with adults rarely accounting for more than 10% of zooplankton densities. The most important adult copepod was Tropocyclops, as was the case in previous years. Bosmina was the predominant cladoceran, as has also been the case in most previous years of the Program. Bosminopsis dominated most cladoceran populations in August. The most abundant rotifers observed in 2004, as in many previous years, were Polyarthra,Conochilus, and Karetella, while Asplanchna and Syncheata were occasionally important among rotifer populations.

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FISHERIES In accordance with the Lake Norman Maintenance Monitoring Program for the NPDES permit for MNS, specific fish monitoring programs were coordinated with the NCWRC and continued during 2004. Spring electrofishing indicated that 17 to 21 species of fish and 2 hybrid complexes comprised fish populations in the 3 sampling areas, and numbers and biomass of fish in 2004 were generally similar to those noted since 1993. Declines in largemouth bass numbers, which were first observed in 2000, appear to be an exception.

In 2004, considerable striped bass mortality was observed during summer in Lake Norman and this mortality appeared to be related to a combination of unique events triggered by an unusually warm May and the abundance of prey in the hypolimnion. Mean Wr for Lake Norman striped bass collected in November and December 2004 was similar to that observed previously and indicated little change in the overall condition of this fish.

Trapnetting indicated little change in the crappie populations in Lake Norman in 2003-2004.

Hydroacoustic and purse seine sampling indicated that there was a decline in the number of prey fish and a change in species composition from 2003 to 2004.

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LIST OF TABLES Table # Title Page #

1-1 Average monthly capacity factors for McGuire Nuclear Station 1-2 2-1 Water chemistry program for McGuire Nuclear Station 2-18 2-2 Water chemistry analytical methods and reporting limits 2-19 2-3 Heat content calculations for Lake Norman in 2003 and 2004 2-20 2-4 Comparison of Lake Norman with TVA reservoirs 2-21 2-5 Lake Norman water chemistry data in 2003 and 2004 2-22 3-1 Mean chlorophyll a concentrations and secchi depths in Lake Norman 3-13 3-2 Duncan's multiple range test for chlorophyll a 3-14 3-3 Total phytoplankton densities and biovolumes from Lake Norman 3-15 3-4 Duncan's multiple range test for phytoplankton densities 3-16 3-5 Duncan's multiple range test for dry and ash free dry weights 3-17 3-6 Phytoplankton taxa identified in Lake Norman from 1990 - 2004 3-18 3-7 Dominate classes and species of Phytoplankton 3-28 4-1 Total zooplankton densities and composition 4-10 4-2 Duncan's multiple range test for zooplankton densities 4-12 4-3 Zooplankton taxa identified in Lake Norman from 1987 - 2004 4-13 4-4 Dominant taxa and percent composition of selected zooplankton 4-16 5-1 Common and scientific names of fish collected in Lake Norman 5-11 5-2 Numbers and biomass of fish collected in April, 2004 5-12 5-3 Lengths and age of largemouth bass collected in Lake Norman 5-13 5-4 Comparison of length and age for largemouth bass from near MSS and 5-13 MNS from data collected 1971 - 2004 5-5 Dead or dying striped bass in Lake Norman July-August 2004 5-14 5-6 Fish collect from gill nets in July 2004 5-16 5-7 Prey fish densities from September 2004 5-16 5-8 Prey fish statistics from purse seine samples collected late summer or 5-17 early fall 1997 - 2004 vi

LIST OF FIGURES Figure # Title Page 2-1 Map of sampling locations on Lake Norman 2-25 2-2a Annual precipitation totals near McGuire Nuclear Station, 1975 - 2004 2-26 2-2b Monthly precipitation totals near McGuire Nuclear Station for 2003 and 2-26 2004 2-2c Monthly mean air temperatures (1989 - 2004) near McGuire Nuclear 2-27 Station 2-3 Monthly mean temperature profiles in background zone 2-28 2-4 Monthly mean temperature profiles in mixing zone 2-30 2-5 Monthly temperature and dissolved oxygen at the MNS discharge 2-32 2-6 Monthly mean dissolved oxygen profiles in background zone 2-33 2-7 Monthly mean dissolved oxygen profiles in mixing zone 2-35 2-8 Monthly temperature isotherms for Lake Norman 2-37 2-9 Monthly dissolved oxygen isopleths for Lake Norman 2-40 2-1 Oa Heat content of Lake Norman 2-43 2-1Ob Dissolved oxygen content of Lake Norman 2-43 2-11 Striped bass habitat in Lake Norman 2-44 2-12 Lake levels for 2002, 2003, and 2004 for Lake Norman 2-46 3-1 Chlorophyll a measurements, densities, biovolumes, and seston weights 3-29 3-2 Mean chlorophyll a concentrations for 1987 - 2004 3-30 3-3 Chlorophyll a concentrations by location for 1988 - 2004 for February 3-31 3-4 Chlorophyll a concentrations by location for 1987 - 2004 for August 3-32 and November 3-5 Class composition of phytoplankton at Locations 2.0 3-33 3-6 Class composition of phytoplankton at Locations 5.0 3-34 3-7 Class composition of phytoplankton at Location 9.5 3-35 3-8 Class composition of phytoplankton at Location 11.0 3-36 3-9 Class composition of phytoplankton at Location 15.9 3-37 3-10 Myxophycean index values from 1988 - 2004 3-38 4-1 Zooplankton density by sample location in Lake Norman 4-18 4-2 Zooplankton community composition in Lake Norman 4-19 4-3 Zooplankton densities during February and May 1988 - 2004 4-20 4-4 Zooplankton densities during August and November 1987 - 2004 4-21 4-5 Quarterly zooplankton community composition from 1990 - 2004 4-22 4-6 Annual lake-wide zooplankton community composition, 1988 - 2004 4-23 4-7 Zooplankton community composition from mixing zone locations, 1988 4-24

- 2004 4-8 Zooplankton composition from background locations, 1988 - 2004 4-25 5-1 Lake Norman sampling zones 5-18 Vii

5-2 Numbers and biomass of fish in Lake Norman 1993 - 2004 5-19 5-3 Numbers and biomass of largemouth bass in Lake Norman 1993 - 2004 5-20 5-4 Size distributions of largemouth bass in Lake Norman in 2004 5-21 5-5 Relative weights for largemouth bass in Lake Norman in 2004 5-22 5-6 Lengths and relative weights for striped bass in Lake Norman 5-23 5-7 Lakewide population estimates for pelagic fish 5-24 5-8 Size distribution of forage fish from purse seine surveys 5-25 viii

CHAPTER 1 McGUIRE NUCLEAR STATION OPERATION INTRODUCTION As required by the National Pollutant Discharge Elimination System (NPDES) permit number NC0024392 for McGuire Nuclear Station (MNS) issued by the North Carolina Department of Environment and Natural Resources (NCDENR), the following annual report has been prepared. This report summarizes environmental monitoring of Lake Norman conducted during 2004.

OPERATIONAL DATA FOR 2004 The monthly average capacity factor for MNS was 101.3 %, 101.2 %, and 101.9 % during July, August, and September of 2004, respectively (Table 1-1). These are the months when conservation of cool water and discharge temperatures are most critical and the thermal limit for MNS increases from a monthly average of 95.0 'F (35.0 'C) to 99.0 'F (37.2 'C). The average monthly discharge temperature was 97.7 'F (36.5 'C) for July, 97.6 'F (36.4 0C) for August, and 94.2 'F (34.6 0 C) for September 2004. The volume of cool water in Lake Norman was tracked throughout the year to ensure that an adequate volume was available to comply with both the Nuclear Regulatory Commission Technical Specification requirements and the NPDES discharge water temperature limits.

1-1

Table 1-1. Average monthly capacity factors (%) and monthly average discharge water temperatures for McGuire Nuclear Station during 2004.

MONTHLY AVERAGE MONTHLY AVERAGE CAPACITY FACTORS (%) NPDES DISCHARGE TEMPERATURES Month Unit 1 Unit 2 Station OF °C January 105.1 105.1 105.1 68.0 20.0 February 105.1 104.9 105.0 67.5 19.7 March 16.7 104.8 60.8 67.9 19.9 April 61.1 104.3 82.7 73.3 22.9 May 103.9 103.7 103.8 84.0 28.9 June 102.2 102.3 102.3 93.1 33.9 July 101.3 101.3 101.3 97.7 36.5 August 101.3 101.0 101.2 97.6 36.4 September 101.9 102.0 101.9 94.2 34.6 October 55.7 103.3 79.5 84.2 29.0 November 64.7 104.0 84A 78.1 25.6 December 105.1 104.6 104.9 75.2 24.0

[ Averages 85.3 103.4 94A 81.7 27.6 1-2

CHAPTER 2 WATER CHEMISTRY INTRODUCTION The objectives of the water chemistry portion of the McGuire Nuclear Station (MNS)

NPDES Maintenance Monitoring Program are to:

1. maintain continuity in Lake Norman's chemical data base to allow detection of any significant station-induced and/or natural change in the physicochemical structure of the lake; and
2. compare, where applicable, these physicochemical data to similar data in other hydropower reservoirs and cooling impoundments in the Southeast.

This report focuses primarily on 2003 and 2004. Where appropriate, reference to pre-2003

.data will be made by citing reports previously submitted to the North Carolina Department of Environment and Natural Resources (NCDENR).

METHODS AND MATERIALS The complete water chemistry monitoring program for 2004, including specific variables, locations, depths, and frequencies is outlined in Table 2-1. Sampling locations are identified in Figure 2-1, whereas specific chemical methods and associated analytical reporting limits, along with the appropriate references, are presented in Table 2-2. Measurements of temperature, dissolved oxygen (DO), DO saturation, pH, and specific conductance were taken, in situ, at each location with a Hydrolab Data-Sonde (Hydrolab 1986) starting at the lake's surface (0.3m) and continuing at lm intervals to one meter above lake bottom. Pre and post-calibration procedures associated with operation of the Hydrolab were strictly followed, and documented in hard-copy format. Hydrolab data were captured and stored electronically, and following a data validation step, converted to spreadsheet format for permanent filing.

2-1

Water samples for laboratory analysis were collected with a Kemmerer water bottle at the surface (0.3m), and from one meter above bottom, where specified (Table 2-1). Samples not requiring filtration were placed directly in single-use polyethylene terephthalate (PET) bottles which were pre-rinsed in the field with lake-water just prior to obtaining a sample.

Samples for the analysis of soluble nutrients (orthophosphate, nitrite-nitrate, and ammonia) were processed in the field by filtering a known volume of water through a 0.45um glass-fiber filter which was pre-rinsed in the field with a 100 mL portion of the filtrate. Upon collection, all water samples were immediately preserved and stored in the dark and on ice to minimize the possibility of physical, chemical or microbial transformation.

Water quality data were subjected to various graphical and statistical techniques in an attempt to describe spatial and temporal trends within the lake, and interrelationships among constituents. Whenever analytical values were obtained that were equal to or less than the method reporting limit, these values were set equal to the reporting limit for statistical purposes. Data were analyzed using two approaches, both of which were consistent with earlier Duke Power Company (DPC), and Duke Power (DP) studies on the lake (DPC 1985, 1987, 1988, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004). The first method involved partitioning the reservoir into mixing, background, and discharge zones, consolidating the data into these sub-sets, and making comparisons among zones and years. In this report, the discharge includes only Location 4; the mixing zone, Locations 1 and 5; the background zone includes Locations 8, 11, and 15.

The second approach, applied primarily to the in-situ data, emphasized a much broader lake-wide investigation and encompassed the plotting of monthly isotherms and isopleths, and summer-time striped bass habitat. Several quantitative calculations were also performed on the in-situ Hydrolab data; these included the calculation of the areal hypolimnetic oxygen deficit (AHOD), maximum whole-water column and hypolimnion oxygen content, maximum whole-water column and hypolimnion heat content, mean epilimnion and hypolimnion heating rates over the stratified period, and the Birgean heat budget.

Heat content (Kcal/cm ), oxygen content (mg/cm 2 ), and mean oxygen concentration (mg/L) of the reservoir were calculated according to Hutchinson (1957), using the following equation:

Lt = Ao-I TO

  • Az
  • dz where; 2-2

Lt = reservoir heat (Kcal/cm ) or oxygen (mg/cm 2 ) content Ao = surface area of reservoir (cm )

TO = mean temperature (fC) or oxygen content of layer z Az = area (cm2 ) at depth z dz= depth interval (cm) z= surface Zm = maximum depth Precipitation and air temperature data were obtained from a meteorological monitoring site established in lower Lake Norman, near MNS, in 1975. These data are employed principally by Duke Power as input variables into meteorological modeling studies to address safety issues associated with potential radiological releases into the atmosphere by MNS (Duke Energy Company 2004), as required by the Nuclear Regulatory Commission. The data also serve to document localized temporal trends in air temperatures and rainfall patterns.

Data on lake level and hydroelectric flows were obtained from Duke Power's Fossil/Hydroelectric Department, which monitors these metrics hourly.

RESULTS AND DISCUSSION Precipitation and Air Temperature Annual precipitation in the vicinity of MNS in 2004 totaled 44.6 inches (Figures 2-2a, b) or 17.1 inches less than observed in 2003 (61.7 inches), but similar to the long-term precipitation average for this area (46.3 inches), based on Charlotte, NC airport data. In 2003, greater than 80% of the yearly rainfall occurred over the first eight months of the year.

In contrast, greater than 80% of the yearly rainfall in 2004 occurred over the last eight months of the year. The highest total monthly rainfall in 2004 occurred in September (7.73 inches), in concert with the occurrence of Hurricanes Frances and Ivan, both of which bypassed the greater Charlotte area but exerted a considerable effect on the North Carolina mountains and foothills.

Air temperatures in 2004 were generally warmer than measured in 2003, and the long-term mean, based on monthly average data (Figure 2-2c). The temporal difference was most pronounced in May when 2004 temperatures averaged 3.5 0 C warmer than 2003, and 2.6 'C warmer than the long-term average.

2-3

Temperature and Dissolved Oxygen Water temperatures measured in 2004 illustrated similar temporal and spatial trends in the background and mixing zones (Figures 2-3 and 2-4), as they did in 2003. This similarity in temperature patterns between zones has been a dominant feature of the thermal regime in Lake Norman since MNS began operations in 1983.

Water temperatures in 2004 were generally warmer than those observed in 2003 in both the mixing and background zone, and paralleled interannual differences exhibited in air temperatures (Figures. 2-2c, 2-3, and 2-4). Minimum water temperatures in 2004 were recorded in early February and ranged from 6.6 'C to 8.0 'C in the background zone, and from 7.4 'C to 13.1 0 C in the mixing zone. The average minimum temperature in 2004 was about 0.3 0 C warmer than that observed in 2003, and corresponded closely with the between-year difference in mean winter air temperature. Minimum water temperatures measured in 2004 were within the observed historical variability (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004).

Spring and summer water temperatures in 2004 ranged from about 1 to 5 0C warmer, on the average, than observed in 2003, with the primary differences occurring in the upper lOin of the water column (Figure 2-3, 2-4). The greatest between-year variability in summer water temperature was observed in June in both the mixing and background zones. Water temperatures in this portion of the water column ranged from 3.8 to 6.2 'C warmer in 2004 than 2003, and can be traced to the antecedent May air temperatures, which were the warmest recorded over the last 40 years (unpublished data, Charlotte airport). Maximum August surface water temperatures were also greater in 2004 than 2003 with interannual differences observed within each zone. The maximum surface temperature in the background zone in 2004 was 29.7 'C, whereas in 2003 the corresponding maximum temperature was 28.5 'C, or almost 1.2 'C cooler. Similarly, the maximum August surface temperature in the mixing zone in 2004 was 30.8 'C compared to a maximum of 29.1 0 C in 2003. Minimal differences in hypolimnetic (below 20m) temperatures were observed between 2004 and 2003 during the summer; the lone exception was in September when the deeper waters were warmer (and the surface waters were cooler) than observed in 2003, especially in the background zone. These thermal differences can be explained by differential cooling of the water column in 2004 versus 2003, in response to lower air temperatures in the preceding month of August (Figure 2-2c).

2-4

Fall and early winter water temperatures (October, November and December) in 2004 were generally similar to those measured in 2003, and followed the trend exhibited in air temperatures (Figure 2-3) Some differences were observed between years, and in certain portions of the water column, but overall cooling of the water column proceeded at a similar rate in 2004 and 2003.

Temperature data at the discharge location in 2004 were generally similar to 2003 (Figure 2-

5) and historically (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004). Temperatures in 2004 were typically equal to or cooler (by a maximum of 6.1 'C) in the spring, and warmer (by a maximum of 3.7 'C) in the summer and fall, than observed in 2003. The warmest discharge temperature of 2004 at Location 4 occurred in August and measured 38.8 'C, or 3.7 'C warmer than measured in August, 2003 (DP 2004).

Seasonal and spatial patterns of DO in 2004 were reflective of the patterns exhibited for temperature, i.e., generally similar in both the mixing and background zones (Figures 2-6 and 2-7). As observed with water column temperatures, this similarity in dissolved DO patterns between zones has been a dominant feature of the oxygen regime in Lake Norman since MNS began operations in 1983.

Winter and early spring DO values in 2004 were generally equal to or slightly lower, in both the background and mixing zones, than measured in 2003, except in January in the background zone which exhibited slightly higher oxygen concentrations in 2004 versus 2003 (Figures 2-6 and 2-7). The interannual differences in DO values measured during this period appeared to be related predominantly to the warmer water column temperatures in 2004 versus 2003. Warmer water would be expected to exhibit a lesser oxygen content because of the direct effect of temperature on oxygen solubility, which is an inverse relationship, and indirectly via a restricted convective mixing regime which would limit water column reaeration. February and March 2004 DO concentrations were about equal to or 0.5 mg/L less throughout the water column than measured in 2003.

Spring and summer DO values in 2004 were highly variable throughout the water column in both the mixing and background zones ranging from highs of 6 to 8 mg/L in surface waters to lows of 0 to 2 mg/L in bottom waters. This pattern is similar to that measured in 2003 and earlier years (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004). Epilimnetic and metalimnetic DO 2-5

values during the spring and summer of 2004 were typically slightly lower than measured at similar depths in 2003, especially in the background zone, although exceptions to this were observed in both zones. In both the mixing and background zones, August, 2004 DO concentrations in the waters above 10m were considerably higher than measured in 2003 even though temperatures were warmer. Hypolimnetic DO values measured during this period were also either equal to or slightly greater than measured in 2003 in both the mixing and background zones. All dissolved oxygen values recorded in 2004 were within the historical range (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004).

Considerable differences were observed between 2004 and 2003 fall DO values in both the mixing and background zone, especially in the metalimnion and hypolimnion during the months of September, October and November (Figures 2-6 and 2-7). These interannual differences in autumn DO levels are common in Catawba River reservoirs and can be explained by the effects of variable weather patterns on water column cooling and mixing.

Warmer air temperatures would delay water column cooling (Figure 2-3, 2-4) which, in turn, would delay the onset of convective mixing of the water column and the resultant reaeration of the metalimnion and hypolimnion. Conversely, cooler air temperatures would promote the rate and magnitude of this process resulting in higher DO values earlier in the year. The 2004 autumn DO data indicate that fall reaeration proceeded faster and was more complete throughout the water column than observed in the corresponding months in 2003.

Consequently, DO levels in these months in 2004 were higher than observed in 2003.

Interannual differences in DO patterns are common not only within the Catawba River Basin, but throughout Southeastern reservoirs, and can reflect yearly differences in hydrologic, meteorologic, and limnologic forcing variables (Cole and Hannon 1985; Petts, 1984).

The seasonal pattern of DO in 2004 at the discharge location was similar to that measured historically, with the highest values observed during the winter and lowest observed in the summer and early fall (Figure 2-5). The lowest DO concentration measured at the discharge location in 2004 (5.55 mg/L) occurred in July and August, and was 1.45 mg/L higher than measured in August 2003 (4.1 mg/L) and similar to DO levels measured in August 2002 (5.4 mg/L), and August 2001 (5.5 mg/L).

2-6

Reservoir-wide Temperature and Dissolved Oxygen The monthly reservoir-wide temperature and DO data for 2004 are presented in Figures 2-8 and 2-9. These data are similar to that observed in previous years and are characteristic of cooling impoundments and hydropower reservoirs in the Southeast (Cole and Hannon, 1985; Hannon et. al., 1979; Petts, 1984). For a detailed discussion on the seasonal and spatial dynamics of temperature and dissolved oxygen during both the cooling and heating periods in Lake Norman, the reader is referred to earlier reports (DPC 1992, 1993, 1994, 1995, 1996).

The seasonal heat content of both the entire water column and the hypolimnion for Lake Norman in 2004 are presented in Figure 2-1 Oa; additional information on the thermal regime in the reservoir for the years 2003 and 2004 is found in Table 2-3. Annual minimum heat content for the entire water column in 2004 (7.92 Kcal/cm 2 ; 8.0 0 C) occurred in early February, whereas the maximum heat content (29.72 Kcal/cm 2 ; 28.99 0 C) occurred in early July. Heat content of the hypolimnion exhibited a somewhat different temporal trend as that observed for the entire water column. Annual minimum hypolimnetic heat content occurred in early February and measured 4.40 Kcal/cm2 (7.0 0 C), whereas the maximum occurred in mid-September and measured 16.18 Kcal/cm 2 (23.6 0 C). Heating of both the entire water column and the hypolimnion occurred at approximately a linear rate from minimum to maximum heat content. The mean heating rate of the entire water column equaled 0.118 Kcal/cm 2 /day and 0.054 Kcal/cm 2 /day for the hypolimnion. The 2004 heat content and heating rate data were similar to that observed in previous years (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004).

The seasonal oxygen content and percent saturation of the whole water column, and the hypolimnion, are depicted for 2004 in Figure 2-lOb. Additional oxygen data can be found in Table 2-4 which presents the 2004 AHOD for Lake Norman and similar estimates for 18 Tennessee Valley Authority (TVA) reservoirs. Reservoir oxygen content was greatest in mid-winter when DO content measured 10.9 mg/L for both the whole water column and the hypolimnion. Percent saturation values at this time approached 98 % for the entire water column and 94 % for the hypolimnion. Beginning in early spring, oxygen content began to decline precipitously in both the whole water column and the hypolimnion, and continued to decline linearly until reaching a minimum in mid summer. Minimum summer volume-weighted DO values for the entire water column measured 4.6 mg/L (60 % saturation),

2-7

whereas the minimum for the hypolimnion was 0.3 mg/L (3.8 % saturation). The mean rate of DO decline in the hypolimnion over the stratified period, i.e., the AHOD, was 0.046 mg/cm 2 /day (0.058 mg/L/day) (Figure 2-lOb), and is similar to that measured in 2003 (DP 2004).

Hutchinson (1938, 1957) proposed that the decrease of DO in the hypolimnion of a waterbody should be related to the productivity of the trophogenic zone. Mortimer (1941) adopted a similar perspective and proposed the following criteria for AHODs associated with various trophic states; oligotrophic - < 0.025 mg/cm 2 /day, mesotrophic - 0.026 mg/cm 2 /day to 0.054 mg/cm 2 /day, and eutrophic - 2 0.055 mg/cm 2 /day. Employing these limits, Lake Norman should be classified as mesotrophic based on the calculated AHOD value of 0.046 mg/cm2 /day for 2004. The oxygen based mesotrophic classification agrees well with the mesotrophic classification based on chlorophyll a levels (Chapter 3). The 2004 AHOD value is also similar to that found in other Southeastern reservoirs of comparable depth, chlorophyll a status, and secchi depth (Table 2-4).

Striped Bass Habitat Suitable pelagic habitat for adult striped bass, defined as that layer of water with temperatures < 26 'C and DO levels 2 2.0 mg/L, was found lake-wide from mid September 2003 through early July 2004. Beginning in mid June 2004, habitat reduction proceeded rapidly throughout the reservoir both as a result of deepening of the 26 'C isotherm and metalimnetic and hypolimnetic deoxygenation (Figure 2-11). Habitat reduction was most severe from mid July through early September when no suitable habitat was observed in the reservoir except for a small, but variable, zone of refuge in the upper, riverine portion of the reservoir, near the confluence of Lyles Creek with Lake Norman. Habitat measured in the upper reaches of the reservoir appeared to be influenced by both inflow from Lyles Creek and discharges from Lookout Shoals Hydroelectric facility, which were somewhat cooler than ambient conditions in Lake Norman. Upon entering Lake Norman, this water apparently mixes and then proceeds as a subsurface underflow as it migrates downriver (Ford 1985).

A secondary temporary refuge was also observed in the hypolimnion near the dam during this period, but this lasted only until late July when dissolved oxygen was reduced to < 2.0 mg/l by microbial demands. The reservoir was completely devoid of habitat for adult striped bass from 27 July to 9 August, or about two weeks. These habitat conditions were 2-8

marginally better than observed in most previous years, including 2003 which exhibited complete habitat elimination for a period of about 30-35 days.

Physicochemical habitat was observed to have expanded appreciably by mid September, primarily as a result of epilimnion cooling and deepening, and in response to changing meteorological conditions. The temporal and spatial pattern of striped bass habitat expansion and reduction observed in 2004 was generally similar to that previously reported in Lake Norman, and many other Southeastern reservoirs (Coutant 1985, Matthews et al. 1985, DPC 1992, 1993, 1994, 1995, 1996, 1997, DP 1998, 1999, 2000, 2001, 2002, 2003, 2004).

However, despite overall similarities in the pattern, rate, and extent of habitat depletion in 2004 versus previous years, a significant die-off of striped bass (2610 fish) was observed in 2004. A detailed account of this phenomenon is found in Chapter 5.

Turbidity and Specific Conductance Surface turbidity values were generally low at the MNS discharge, mixing zone, and mid-lake background locations during 2004, ranging from 1.0 to 3.4 NTU's (Table 2-5). Bottom turbidity values were also relatively low over the study period, ranging from 0.9 to 7.5 NTU's (Table 2-5). Turbidity values observed in 2004, as a whole, were slightly lower than measured in 2003 (Table 2-5), but well within the historical range (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004).

Specific conductance in Lake Norman in 2004 ranged from 50 to 100 umho/cm, and was generally similar to that observed in 2003 (Table 2-5), and historically (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004). Lake-wide, mean conductance values in 2004 were similar to those measured in 2003, but were about 10 umhos/cm lower than observed in 2002. Differences were most pronounced in the surface waters during the summer with mean conductance values averaging almost 20 umhos/cm (about 30 %) less in 2004 and 2003 than 2002. These differences appear to have been related to interannual variability in watershed precipitation totals (Figures 2-2a & 2-2b) and the corresponding influence on reservoir water level (Figure 2-12) and volume, as well as inflow and outflow rates. Precipitation totals for the Lake Norman watershed, as recorded at MNS, were considerably less in 2002 than 2003 and 2004.

Reduced levels of precipitation inputs within the watershed would be expected to result in less sub-surface and overland runoff to adjacent streams and lakes. Reduced stream inflows 2-9

and lake levels would, in turn, concentrate chemical constituents within the water column.

Conversely, increases in stream inflows and lake levels would be expected to result in an initial increase in some constituents as material is transported from the terrestrial environment to the adjacent aquatic ecosystem, followed by a decrease in constituent concentration due to dilution associated with additional increases in water volume and corresponding movement through the system (Gray 1970, Kazmann 1988).

Specific conductance values in surface and bottom waters in 2004 were generally similar throughout the year except during the period of intense thermal stratification, i.e., August through November when an increase in bottom conductance values was observed. These increases in bottom conductance values appeared to be related primarily to the release of soluble iron and manganese from the lake bottom under anoxic conditions (Table 2-5). This phenomenon is common in both natural lakes and reservoirs that exhibit extensive hypolimnetic oxygen depletion (Hutchinson 1957, Wetzel 1975).

pH and Alkalinity During 2004, pH and alkalinity values were similar among MNS discharge, mixing and background zones (Table 2-5). Values of pH were also generally similar to values measured in 2003 (Table 2-5), and historically (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004). Alkalinity values were also similar between years. Individual pH values in 2004 ranged from 6.1 to 8.0, whereas alkalinity ranged from 12.5 to 36 mg/L, expressed as CaCO 3 .

Maior Cations and Anions The concentrations of major ionic species in the MNS discharge, mixing, and mid-lake background zones are provided in Table 2-5. Lake-wide, the major cations were sodium, calcium, magnesium, and potassium, whereas the major anions were bicarbonate, sulfate, and chloride. The overall ionic composition of Lake Norman during 2004 was generally similar to that reported for 2003 (Table 2-5) and previously (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004).

Concentrations of several constituents, most notably calcium and potassium, and to a lesser extent chloride, magnesium, and sodium, were however, consistently less in 2004 than 2003, but these differences were not statistically significant and values were within historical ranges.

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Nutrients Nutrient concentrations in the discharge, mixing, and mid-lake background zones of Lake Norman for 2004 and 2003 are provided in Table 2-5. Overall, nutrient concentrations in 2004 were well within historical ranges (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004). Nitrogen and phosphorus levels in 2004 were low and generally similar to those measured in 2003 (DP 2004). Total phosphorus and ortho-phosphorus concentrations were typically measured at or below the analytical reporting limits for these constituents, i.e., 10 ug/L and 5 ug/L, respectively. For total phosphorus, only three of the forty samples analyzed exceeded a concentration greater than 10 ug/L, and the highest concentration measured was 12 ug/L.

For ortho-phosphorus eight of forty samples exceeded 5 ug/L, and the highest concentration measured was 13 ug/L in the bottom waters of the mixing zone in November. Nutrients were generally higher in the upper portions of the reservoir compared to the lower sections, but the differences were slight and not statistically significant (p< 0.05). Spatial variability in various chemical constituents, especially nutrient concentrations, is common in long, deep reservoirs (Soballe et al. 1992).

Nitrite-nitrate concentrations were consistently lower at all locations in 2004 compared to 2003 (Table 2-5), but similar to historical values (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004). The lower values measured in 2004 compared to 2003 were probably related primarily to interannual differences in precipitation inputs (Figure 2-2a). Nitrate-nitrite concentrations in atmospheric precipitation in this portion of North America typically range from about 600 to 1000 ug/L; consequently, rainfall serves as a significant source of nitrogen to aquatic ecosystems in the Southeast both in the form of direct and indirect inputs (Langmuir 1997).

Metals Metal concentrations in the discharge, mixing, and mid lake background zones of Lake Norman for 2004 were similar to those measured in 2003 (Table 2-5) and historically (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004). Iron concentrations in surface and bottom waters were generally low (< 0.2 mg/L) during 2004, the lone exception being a 0.63 mg/L value measured in the bottom waters at Location 5 in August. Nowhere in the reservoir in 2004 did iron concentrations exceed NC's water quality standard (NCDENR 2004) for this 2-11

constituent (1.0 mg/L), which is unusual. Typically, iron concentrations increase in the bottom waters during the late summer, and early fall, in response to changing redox conditions (see below). It's unclear why this phenomenon was not as prevalent in 2004 as in previous years.

Similarly, manganese concentrations in the surface and bottom waters were generally low (<

100 ug/L) in 2004, except during the summer and fall when bottom waters were anoxic (Table 2-5). This phenomenon, i.e., the release of manganese (and iron) from bottom sediments in response to low redox conditions (low oxygen levels), is common in stratified waterbodies (Stumm and Morgan 1970, Wetzel 1975). Manganese concentrations in the bottom waters rose above NC's water quality standard (NCDENR 2004) for this constituent, i.e., 200 ug/L, at various locations throughout the lake in summer and fall in 2004, and is characteristic of historical conditions (DPC 1985, 1987, 1988a, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; DP 1998, 1999, 2000, 2001, 2002, 2003, 2004).

Concentrations of other metals in 2004 were typically low, and often below the analytical reporting limit for the specific constituent (Table 2-5). All values for cadmium, lead and zinc were reported as either equal to or below each constituent's reporting limit, and no NC water quality standard was exceeded. All copper concentrations were less than 3 ug/L, and well below the NC standard of 7 ug/L (NCDENR 2004).

FUTURE STUDIES No changes are planned for the Water Chemistry portion of the Lake Norman maintenance-monitoring program.

SUMMARY

Annual precipitation in the vicinity of MNS in 2004 totaled 44.6 inches or 17.1 inches less than observed in 2003, but similar to the long-term precipitation average for this area (46.3 inches). Air temperatures in 2004 were generally warmer than measured in 2003, as well as the long-term mean. The most pronounced differences occurred in May when 2004 temperatures averaged 3.5 0 C warmer than 2003, and 2.6 'C warmer than the long-term average.

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Temporal and spatial trends in water temperature and DO in 2004 were similar to those observed historically, and all data were within the range of previously measured values.

Water temperatures in 2004 were generally warmer than observed in 2003 in both the mixing and background zones. Winter temperatures averaged about 0.3 'C warmer throughout the water column in 2004 versus 2003. Summer temperatures averaged about 2 to 3 'C warmer in 2004 versus 2003, with the primary differences observed in the upper 10 m of the water column. Minimal differences in hypolimnetic (below 20 m) temperatures were observed between 2004 and 2003 during the summer; the lone exception was in September when the lower waters were warmer than observed in 2003, especially in the background zone. These thermal differences can be explained by differential cooling of the water column in 2004 versus 2003, in response to lower air temperatures in the preceding month of August.

Interannual differences in water temperatures, especially in the surface waters, typically paralleled differences exhibited in air temperatures between 2004 and 2003.

Winter and early spring DO values in 2004 were generally equal to or slightly lower, in both the background and mixing zones, than measured in 2003, except in January in the background zone which exhibited slightly higher oxygen concentrations in 2004 versus 2003.

The interannual differences in DO values measured during this period appeared to be related predominantly to the warmer water column temperatures in 2004 versus 2003.

Spring and summer DO values in 2004 were highly variable throughout the water column in both the mixing and background zones ranging from highs of 6 to 8 mg/L in surface waters to lows of 0 to 2 mg/L in bottom waters. This pattern is similar to that observed in 2003 and earlier years. Epilimnetic and metalimnetic DO values during the spring and summer of 2004 were typically slightly lower than measured at similar depths in 2003, especially in the background zone, although exceptions to this were observed in August in both zones.

Summer hypolimnetic DO values were also either equal to or slightly greater than measured in 2003 in both the mixing and background zones. All DO values recorded in 2004 were within historical ranges.

Reservoir-wide isotherm and isopleth information for 2004, coupled with heat content and hypolimnetic oxygen data, illustrated that Lake Norman exhibited thermal and oxygen dynamics characteristic of historical conditions and similar to other Southeastern reservoirs of comparable size, depth, flow conditions, and trophic status. Availability of suitable pelagic habitat for adult striped bass in Lake Norman in 2004 was generally similar in distribution and amount to historical conditions observed annually since 1983. Despite 2-13

similarities in habitat conditions to previous years, the largest striped bass die-off ever observed in the reservoir (2599 fish) occurred in the summer of 2004.

All chemical parameters measured in 2004 were within the concentration ranges previously reported for the lake during both preoperational and operational years of MNS. Specific conductance values in 2004 were slightly lower than measured in 2003, a high-water year, as were concentrations of nitrite-nitrate nitrogen, calcium and potassium, and to a lesser extent, chloride, magnesium and sodium. Ammonia, ortho-phosphorus, and total phosphorus values were low in 2004 and similar to 2003.

Concentrations of metals in 2004 were also low, and often below the analytical reporting limits. Values for cadmium, lead and zinc were reported as either equal to or below the reporting limit. Copper concentrations were generally less then 3 ug/L, and well below the NC standard of 7 ug/L.

Manganese and iron concentrations in the surface and bottom waters were generally low in 2004, except during the summer and fall when bottom waters became anoxic, and the release of soluble forms of these metals into the water column was observed. In contrast to previous years, at no time during 2004 did iron concentrations exceed NC's water quality standard (1.0 mg/L). Manganese levels, however, did exceed the State standard (200 ug/L) in the bottom waters throughout the lake in the summer and fall, and are characteristic of historical conditions.

LITERATURE CITED American Public Health Association (APHA). 1995. Standard Methods for the Examination of Water and Wastewater. I 9th Edition. APHA, Washington, DC.

Coutant, C. C. 1985. Striped Bass, Temperature, and Dissolved Oxygen: a Speculative Hypothesis for Environmental Risk. Trans. Amer. Fisher. Soc. 114:31-61.

Cole, T. M. and H. H. Hannon. 1985. Dissolved Oxygen Dynamics. In: Reservoir Limnology: Ecological Perspectives. K. W. Thornton, B. L. Kimmel and F. E. Payne editors. John Wiley & Sons. NY.

Duke Energy Company. 2004. McGuire Nuclear Station. Updated Final Safety Analysis Report. Duke Energy Company, Charlotte, NC.

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Duke Power Company. 1985. McGuire Nuclear Station, 316(a) Demonstration. Duke Power Company, Charlotte, NC.

Duke Power Company. 1987. Lake Norman Maintenance Monitoring Program: 1986 Summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1988. Lake Norman Maintenance Monitoring Program: 1987 Summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1989. Lake Norman Maintenance Monitoring Program: 1988 Summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1990. Lake Norman Maintenance Monitoring Program: 1989 Summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1991. Lake Norman Maintenance Monitoring Program: 1990 Summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1992. Lake Norman Maintenance Monitoring Program: 1991 Summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1993. Lake Norman Maintenance Monitoring Program: 1992 Summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1994. Lake Norman Maintenance Monitoring Program: 1993 Summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1995. Lake Norman Maintenance Monitoring Program: 1994 Summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1996. Lake Norman Maintenance Monitoring Program: 1995 Summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1997. Lake Norman Maintenance Monitoring Program: 1996 Summary. Duke Power Company, Charlotte, NC.

Duke Power. 1998. Lake Norman Maintenance Monitoring Program: 1997 Summary.

Duke Energy Corporation, Charlotte, NC.

Duke Power. 1999. Lake Norman Maintenance Monitoring Program: 1998 Summary.

Duke Energy Corporation, Charlotte, NC.

Duke Power. 2000. Lake Norman Maintenance Monitoring Program: 1999 Summary.

Duke Energy Corporation, Charlotte, NC.

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Duke Power. 2001. Lake Norman Maintenance Monitoring Program: 2000 Summary.

Duke Energy Corporation, Charlotte, NC.

Duke Power. 2002: Lake Norman Maintenance Monitoring Program: 2001 Summary.

Duke Energy Corporation, Charlotte, NC.

Duke Power. 2003: Lake Norman Maintenance Monitoring Program: 2002 Summary.

Duke Energy Corporation, Charlotte, NC.

Duke Power. 2004. Lake Norman Maintenance Monitoring Program: 2003 Summary.

Duke Energy Corporation, Charlotte, NC.

Ford, D. E. 1985. Reservoir Transport Processes. In: Reservoir Limnology: Ecological Perspectives. K. W. Thornton, B. L. Kimmel and F. E. Payne editors. John Wiley &

Sons. NY.

Gray, D. M. 1970. Handbook on the Principles of Hydrology. Water Information Center, Inc., National Research Council of Canada.

Hannan, H. H., I. R. Fuchs and D. C. Whittenburg. 1979 Spatial and Temporal Patterns of Temperature, Alkalinity, Dissolved Oxygen and Conductivity in an Oligo-mesotrophic, Deep-storage Reservoir in Central Texas. Hydrobiologia 51 (30); 209-221.

Higgins, J. M. and B. R. Kim. 1981. Phosphorus Retention Models for Tennessee Valley Authority Reservoirs. Water Resources Research, 17:571-576.

Higgins, J. M., W. L. Poppe, and M. L. Iwanski. 1980. Eutrophication Analysis of TVA Reservoirs. In Surface Water Impoundments. H. G. Stefan, Ed. Am. Soc. Civ. Eng.,

NY, pages 412-423.

Hutchinson, G. E. 1938. Chemical Stratification and Lake Morphometry. Proc. Nat. Acad.

Sci., 24:63-69.

Hutchinson, G. E. 1957. A Treatise on Limnology, Volume I Geography, Physics and Chemistry. John Wiley & Sons, NY.

Hydrolab Corporation. 1986. Instructions for Operating the Hydrolab Surveyor Datasonde.

Austin, TX. 105p.

Kazmann, R. G. 1988. Modern Hydrology. The National Water Well Association. Dublin, Ohio.

Langmuir, D. 1997. Aqueous Environmental Geochemistry. Prentice-Hall, Inc. Simon and Schuster/ A Viacom Company. Upper Saddle River, New Jersey 07458.

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Matthews, W. J., L. G. Hill, D. R. Edds, and F. P. Gelwick. 1985. Influence of Water Quality and Season on Habitat use by Striped Bass in a Large southwestern Reservoir. Transactions of the American Fisheries Society 118: 243-250.

Mortimer, C. H. 1941. The Exchange of Dissolved Substances Between Mud and Water in Lakes (Parts I and II). J. Ecol., 29:280-329.

North Carolina Division of Environment and Natural Resources. 2004. Red Book. Surface Waters and Wetland Standards. NC Administrative Code. 15a NCAC 02B.0100,

.0200 and .0300. August 1, 2004. 133pp.

Petts G. E., 1984. Impounded Rivers: Perspectives For Ecological Management. John Wiley and Sons. New York. 326pp.

Soballe, D. M., B. L. Kimmel, R.H. Kennedy, and R. F. Gaugish. 1992. Reservoirs. In:

Biodiversity of the Southeastern United States Aquatic Communities. John Wiley &

Sons, Inc. New York.

Stumm, W. and J. J. Morgan. 1970. Aquatic Chemistry: An Introduction Emphasizing Chemical Equilibria in Natural Waters. Wiley and Sons, Inc. New York, NY 583pp.

U.S. Environmental Protection Agency (USEPA). 1983. Methods for the Chemical Analysis of Water and Wastes. Environmental Monitoring and Support Lab. Office of Research and Development, Cincinnati, Ohio.

Wetzel, R. G. 1975. Limnology. W. B. Saunders Company, Philadelphia Pennsylvania, 743pp.

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d- eC (7

Table 2.1 Water chemistry program for the McGuire Nuclear Station NPDES maintenance monitoring program on Lake Norman.

2004 McGUIRE NPDES SAMPLING PROGRAM PARAMETERS LOCATIONS 1 2 4 5 8 9.5 11 13 14 15 15.9 62 69 72 80 16 DEPTH (m) 33 33 5 20 32 23 27 21 10 23 23 15 7 5 4 3 IN-SITU ANALYSIS Temperature Hydrolab Dissolved Oxygen Hydrolab In-situ measurements are collected monthly at the above locations at Im intervals from 0.3m to Im above bottom.

pH Hydrolab Measurements are taken weekly from July-August for striped bass habitat.

Conductivity Hydrolab NUTRIENT ANALYSES Ammonia AA-Nut Q/TB Q/T,B QfT Q/T,B Q/T,B Q/TB Q/T,B Q/T,B Q/T Q/T,B Q/T,B S/T Nitrate+Nitrite AA-Nut Q/T,B Q/T,B Q/T Q/T,B Q/T,B Q/T,B Q/T,B Q/T,B Q/T Q/T,B Q/T,B S/T Orthophosphate AA-Nut Q/T,B Q/T,B Q/T Q/T,B Q/T,B Q/T,B Q/T,B Q/TB Q/T Q/T,B Q/T,B S/T Total Phosphorus AA-TP,DG-P Q/TB Q/T,B Q/T Q/TB Q/T,B Q/TB Q/TB Q/TB Q/T Q/T,B Q/TB S/T Silica AA-Nut Q/T,B Q/T,B QIT Q/T,B Q/T,B Q/TB Q/T,B Q/T,B Q/T Q/TB Q/T,B S/T Cl AA-Nut Q/T,B Q/T,B QfT Q/T,B Q/T,B Q/T,B Q/T,B QIT,B Q/T Q/T,B QfT,B S/T TKN AA-TKN Q/T,B Q/T,B QfT,B Q/T,B Q/TB Q/T,B Q/T,B Q/T,B Q/T,B Q/T,B Q/T,B Total Organic Carbon TOC Q/T,B Q/T Q/T,B Q/T,B Q/T,B Q/T Dissolved organic carbon DOC Q/T,B Q/T Q/T,B Q/T,B Q/T,B Q/T ELEMENTAL ANALYSES Aluminum ICP-MS-D Q/T.B S/T,B QIT Q/T,B Q/T,B Q/T,B Q/TB Q/T,B Q/T Q/T,B Q/T,B S/T Calcium ICP-24 Q/T,B QIT,B QIT Q/T,B Q/T,B Q/T,B Q/T,B QIT,B Q/T Q/T,B Q/T,B SIT Iron ICP-MS-D QIT,B Q/T,B Q/T Q/T,B Q/T,B QIT,B Q/T,B Q/T,B Q/T Q/T,B Q/T,B SIT Magnesium ICP-24 QIT,B Q/T,B Q/T Q/TB Q/T,B Q/T,B Q/T,B Q/T,B Q/T Q/T,B Q/T,B S/T Manganese ICP-MS-D Q/T,B Q/T,B Q/T Q/TB Q/TB Q/TB Q/TB Q/TB QIT Q/T,B Q/TB S/T Potassium 306-K QIT,B Q/T,B Q/T Q/T,B Q/T,B QIT,B QIT,B Q/T,B Q/T Q/T,B Q/T,B S/T Sodium ICP-24 Q/TB Q/T,B QfT Q/T,B Q/T,B Q/T,B Q/T,B QIT,B Q/T Q/T,B Q/T,B S/T Zinc ICP-MS-D QIT,B Q/TB Q/T QIT,B Q/T,B Q/T,B QIT,B Q/TB Q/T Q/T,B Q/T,B S/T Cadminum ICP-MS-D Q/T,B Q/T Q/T,B Q/T,B QIT Q/T,B S/T Copper (Total Recoverabli ICP-MS-D Q/T,B Q/T Q/T,B Q/T,B Q/T Q/T,B S/T Copper (Dissolved) ICP-MS Q/T,B Q/T QIT,B Q/T,B QIT Q/T,B S/T Lead ICP-MS-D QIT,B Q/T QIT,B Q/T,B Q/T Q/T,B SIT ADDITIONAL ANALYSES Alkalinity T-ALKT Q/TB Q/T,B QIT QIT,B Q/T,B Q/T,B QIT,B QIT,B QIT Q/T,B Q/T,B SIT Turbidity F-TURB Q/T,B QIT,B QIT Q/T,B Q/T,B Q/T,B Q/T,B Q/T,B Q/T Q/T,B Q/T,B S/T Sulfate UV S04 Q/T,B QIT QIT,B Q/T Q/T,B SIT Total Solids S-TSE Q/T,B QIT QIT,B Q/T Q/T,B S/T Total Suspended Solids S-TSSE Q/T,B QIT QIT,B QIT Q/T,B S/T CODES: Frequency Q = Quarterly (Feb. May, Aug, Nov) S = Semi-annually (Feb,Aug) T = Top (0.3m) B = Bottom (Im above bottom) 00

?F1 I% -

Table 2-2. Analytical methods and reporting limits employed in the McGuire Nuclear Station NPDES maintenance monitoring program for Lake Norman.

Parameter Method (EPA/APHA) Preservation Reporting Limit Alkalinity, Total Total inflection point, EPA 310.1 4 C 0.01 meg/L Aluminum ICP, EPA 200.7 0.5% HNO3 0.05 mg/L Cadmium, Total Recoverable ICP Mass Spectroscopy, EPA 200.8 0.5% HNO3 0.5 ug/L Calcium ICP, EPA 200.7 0.5% HNO3 30 ug/L Chloride Colorimetric, EPA 325.2 4C 1.0 mg/L Copper, Total Recoverable ICP Mass Spectroscopy, EPA 200.8 0.5% HNO 3 2.0 ug/L Copper, Dissolved ICP Mass Spectroscopy, EPA 200.8 0.5% HNO 3 2.0 ug/L Iron, Total Recoverable ICP, EPA 200.7 0.5% HNO 3 IO ug/L Lead, Total Recoverable ICP Mass Spectroscopy, EPA 200.8 0.5% HNO 3 2.0 ug/L Magnesium Atomic emission/ICP, EPA 200.7 0.5% HN0 3 30 ug/L Manganese, Total Recoverable ICP Mass Spectroscopy, EPA 200.8 0.5% HNO3 1.0 ug/L Nitrogen, Ammonia Colorimetric, EPA 350.1 4C 20 ug/L Nitrogen, Nitrite + Nitrate Colorimetric, EPA 353.2 4C 20 ug/L Nitrogen, Total Kjeldahl Colorimetric, EPA 351.2 4C 100 ug/L Phosphorus, Orthophosphorus Colorimetric, EPA 365.1 4C 5 ug/L Organic Carbon, Total EPA 415.1 0.5% H2 SO4 0.1 mg/L Organic Carbon, Dissolved EPA 415.1 0.5% H 2SO 4 0.1 mg/L Phosphorus, Total Colorimetric, EPA 365.1 4C 10 ug/L Potassium ICP, EPA 200.7 0.5% HNO 3 250 ug/L Silica APHA 450OSi-F 0.5% HN03 500 ug/L Sodium Atomic emission/ICP, EPA 200.7 0.5% HNO 3 1.5 mg/L Solids, Total Gravimetric, EPA 160.2 4C 0.1 mg/L Solids, Total Suspended Gravimetric, EPA 160.2 4C 0.1 mg/L Sulfate Ion Chromatography 4C 0.1 mg/L Turbidity Turbidimetric, EPA 180.1 4C 0.05 NTU Zinc, Total Recoverable ICP, EPA 200.8 0.5% HNO 3 20 ug/L tsj

References:

USEPA 1983, and APHA 1995

Table 2-3. Heat content calculations for the thermal regime in Lake Norman for 2003 and 2004.

2003 2004 Maximum Areal Heat Content (g-cal/cm 2 ) 28,176 29,718 Minimum Areal Heat Content (g cal/cm 2 ) 8,864 7,921 Birgean Heat Budget (g cal/ cm 2 ) 19,312 21,797 Epilimnion (above 11.5 m) Heating Rate (DC /day) 0.096 0.122 Hypolimnion (below 11.5 m) Heating Rate (0 C /day) 0.072 0.076 2-20

Table 2-4. A comparison of areal hypolimnetic oxygen deficits (AHOD), summer chlorophyll a (chl a), secchi depth (SD), and mean depth of Lake Norman and 18 TVA reservoirs.

AHOD Summer Chl a Secchi Depth Mean Depth Reservoir (mg/cm 2 /day) (ug/L) (m) (m)

Lake Norman (2004) 0.046 5.5 2.2 10.3 TVA a Mainstem Kentucky 0.012 9.1 1.0 5.0 Pickwick 0.010 3.9 0.9 6.5 Wilson 0.028 5.9 1.4 12.3 Wheelee 0.012 4.4 5.3 Guntersville 0.007 4.8 1.1 5.3 Nickajack 0.016 2.8 1.1 6.8 Chickamauga 0.008 3.0 1.1 5.0 Watts Bar 0.012 6.2 1.0 7.3 Fort London 0.023 5.9 0.9 7.3 Tributary Chatuge 0.041 5.5 2.7 9.5 Cherokee 0.078 10.9 1.7 13.9 Douglas 0.046 6.3 1.6 10.7 Fontana 0.113 4.1 2.6 37.8 Hiwassee 0.061 5.0 2.4 20.2 Norris 0.058 2.1 3.9 16.3 South Holston 0.070 6.5 2.6 23.4 Tims Ford 0.059 6.1 2.4 14.9 Watauga 0.066 2.9 2.7 24.5 a Data from Higgins et al. (1980), and Higgins and Kim (1981) 2-21

( F-e IV '7 Table 2-5. Quarterly surface (0.3 m) and bottom (bottom minus I m) water chemistry for the MNS discharge, mixing zone, and background locations on Lake Norman during 2003 and 2004. Values less than detection were assumed to be equal to the detection limit for calculating a mean.

Mixing Zone Mixing Zone MNS Discharge Mixing Zone Background Background LOCATION: 1.0 2 4.0 5.0 8.0 11.0 DEPTH: Surface Bottom Surface Bottom Surface Surface Bottom Surface Bottom Surface Bottom PARAMETERS YEAR: 2004 2003 2004 20031 2004 2003 2004 20031 2004 2003l 2004 2003 2004 2003 2004 2003 2004 200131 2004 2003 2004 2003 Turbidity (ntu)

Feb 2.81 2.69 2.39 2.61 3.12 2.51 1.94 2.65 NS 2.11 2.12 2.03 1.61 2.23 2.02 2.67 3.51 2.56 3.36 4.76 3.36 3.88 May 1.50 4.04 1.52 7.41 NS 5.58 NS 7.32 1.02 8.49 1.38 6.11 NS 7.48 1.25 3.61 1.31 11.00 1.28 2.35 0.94 18.30 Aug 1.45 1.96 2.57 3.90 1.5 1.84 2.09 2.20 1.4 2.03 1.46 2.04 3.63 10.40 1.32 1.82 2.99 6.64 2.11 2.03 2.49 4.38 Nov 2.80 1.56 2.8 2.01 3.13 1.43 3.69 1.88 3.05 1.40 2.98 1.18 7.53 4.25 2.72 1.05 5.77 4.15 3.32 1.25 6.46 5.06 Annual Mean 2.14 2.56 2.32 4.0 2.58 2.8 2.57 3.5 1.82 3.5 1g99 2.8 4.26 6.1 1.83 2.3 3.40 6.1 2.52 2.6 3.31 7.9 Specific Conductance (umho/cm)

Feb 52.0 71 51.0 70 52 71 50 70 53 73 52 71 51.0 70 51 70 50 70 51 67 50 68 May 53.0 60 53 62 57 60 53 62 58 59 57 59 54 61 56 60 54 59 58 58 55 54 Aug 63.0 55 59.0 65 62.0 54 60.0 66 64.0 54 62.0 54 66.0 67 60.0 54 58.0 62 62.0 54 59.0 64 Nov 56.0 55 99.0 95 56.0 54 100.0 97 58.0 55 57.0 55 58.0 58 56.0 53 98.0 54 52.0 53 52.0 52.0 Annual Mean 56.0 60.3 65.5 73.0 56.8 59.8 65.8 73.8 58.3 60.3 57.0 59.8 57.3 64.0 55.8 59.3 65.0 61.3 55.8 58.0 54.0 59.5 pH (units)

Feb 7.0 6.7 7.0 7.0 7.3 7.2 7.1 7.1 7.3 7.1 7.4 7.2 7.1 7.2 7.4 7.3 7.2 7.2 7.3 7.3 7.1 7.2 May 7.2 6.7 6.3 6.5 7.4 6.9 6.6 6.6 7.3 6.8 7.4 6.9 6.6 6.6 7.7 7.2 6.6 6.6 7.7 7.3 6.7 6.5 Aug 7.3 7.1 6.0 6.3 7.4 6.9 6.1 6.3 7.1 6.6 7.3 6.8 6.3 6.5 8.0 7.1 6.1 6.4 7.1 7.3 6.1 6.4 Nov 6.5 6.8 6.8 6.6 NS 6.8 NS 6.7 6.9 6.7 7.0 6.9 6.7 6.6 7.0 6.8 6.9 6.5 7.0 7.2 6.7 6.5 Annual Mean 7.00 6.53 6.53 6.60 7.37 6.95 6.60 6.69 7.15 7.10 7.28 6.95 6.68 6.73 7.53 7.10 6.70 6.67 7.28 7.28 6.65 6.64 Alkalinity (mg CaCO311)

Feb 13.5 16.5 13.0 16.5 13.0 15.5 13.0 15.5 NS 15.5 13.0 16.5 13.0 15.5 13.0 16.0 13.0 15.5 13.0 15.0 13.0 15.5 May 13.5 13.5 13.5 13.0 NS 13.5 NS 14.0 14.0 13.0 13.5 13.0 NS 14.0 13.0 13.5 13.5 13.5 14.0 12.0 13.0 12.0 Aug 15.0 13.0 14.5 14.0 15.0 13.0 14.5 14.0 14.5 13.0 15.0 13.0 20.0 17.5 15.0 13.0 14.5 14.5 15.0 13.5 15.0 14.5 Nov 13.0 14.5 36.0 20.5 13.5 14.5 35.5 29.0 13.5 14.0 13.0 14.5 14.0 16.5 13.0 14.5 15.0 15.5 12.0 14.0 12.5 14.5 Annual Mean 13.8 14.4 19.3 16.0 13.8 14.4 21.0 18.4 14.0 14. 13.6 14.3 15.7 16.1 13.5 14.3 14.0 15.0 13.5 13.6 13.4 14.1 Chloride (mg/I)

Feb 4.0 6.9 4.2 61 4.1 6.7 4.0 7.0 NS 6.7 3.9 6.7 4.1 61 4.1 6.8 4.0 6.E 4.3 6.2 4.1 6.1 May 4.5 5.4 4.5 5.~ NS 5.4 NS 5.8 4.8 5.2 4.6 5.4 NS 5. 4.6 5.3 4.6 5. 5.2 5.2 4.6 4.9 Aug 5.3 4.0 4.8 4.1 5.3 3.9 4.7 4.9 5.4 3.9 5.3 4.0 5.2 4.4 5.4 3.9 4.9 41 5.4 4.1 4.8 .4.7 Nov 4.6 3.9 4.7 4.( 4.5 3.9 4.8 4.6 4.4 3.8 4.4 3.8 4.6 3.t 4.5 4.0 4.5 3.1 4.1 3.9 4.1 3.9 AR Rn AS. Ad a Sulfate (mg/I)

Feb NS NS NS NS 5.0 5.8 4.7 7.2 NS 7.1 NS NS NS NS 4.7 7.3 4.8 7.2 NS NS 6.7 NS May NS NS NS NS NS 6.1 NS 6.3 5.1 5.8 NS NS NS NS 5.1 6.0 5.0 6.0 NS NS NS NS Aug NS NS NS NS 4.6 4.8 4.7 5.6 4.7 4.6 NS NS NS NS 4.6 4.9 4.7 5.4 NS NS NS NS Nov NS NS NS NS 4.5 4.8 3.2 3.3 4.5 4.8 NS NS NS NS 4.5 4.8 4.4 4.2 NS NS NS NS Annual Mean NA NS NAA 4.7 5.4 4.2 56 4.8 5.6 NA NA NA NA 4.7 5.8 4.7 5.7 NA NA 6.7 NA Calcium (mg/l)

Feb 3.12 3.44 3,09 3.43 3.15 3.48 3.10 3.45 NS 3.48 3.09 3.46 3.09 3.46 3.06 3.49 3.12 3.47 2.94 3.67 2.89 3.65 May 2.92 4.62 3.07 4.37 NS 4.11 NS 4.61 3.02 4.18 3.65 3.78 NS 4.42 3.47 3.89 3.24 3.90 3.29 3.38 3.16 3.96 Aug 2.69 4.05 2.97 4.17 2.71 4.07 2.92 3.74 2.73 2.98 2.77 3.22 3.67 5.03 2.73 3.09 3.06 4.07 3.27 4.46 3.15 4.74 Nov 2.99 3.54 4.18 3.84 2.98 3.53 4.10 4.26 3.00 3.48 2.98 3.48 3.03 3.52 2.97 3.46 3.04 3.15 2.78 3.43 2.84 3.10 Annual Mean 2.93 3.91 3.33 3.95 2.95 3.80 3.37 4.02 2.92 3.53 3.12 3.49 3.26 4.11 3.06 3.48 3.12 3.65 3.07 3.74 3.01 3.86 Magnesium (mg/l)

Feb 1.39 1.60 1.37 1.61 1.40 1.60 1.38 1.61 NS 1.62 1.39 1.60 1.39 1.60 1.38 1.60 1.40 1.61 1.33 1.52 1.33 1.55 May 1.37 1.75 1.44 1.70 NS 1.59 NS 1.77 1.41 1.60 1.56 1.52 NS 1.72 1.55 1.58 1.48 1,61 1.45 1.45 1.42 1.56 Aug 1.48 1.63 1.55 1.63 1.49 1.59 1.53 1.57 1.49 1.37 1.51 1.43 1.75 1.82 1.48 1.42 1.57 1.66 1.65 1.73 1.62 1.85 Nov 1.33 1.50 1.68 1.56 1.35 1.49 1.66 1.64 1.34 1.49 1.34 1.49 1.35 1.50 1.34 1.47 1.35 1.43 1.25 1.47 1.25 1.42 Annual Mean 1.39 1.62 1,51 1.63 1.41 1.57 1.52 1.65 1.41 1.52 1.45 1.51 1.50 1.66 1.44 1.52 1.45 1.57 1.42 1.54 1.41 1.60 NS = Not Sampled; NA= Not applicable

C Table 2-5. (Continued)

Mixing Zone Mixing Zone MNS Discharge Mixing Zone Background Background LOCATION: 1.0 2 4.0 5.0 8.0 11.0 DEPTH: Surface Bottom Surface Bottom Surface Surface Bottom Surface Bottom Surface Bottom PARAMETERS YEAR: 2004 2003 2004 20031 2004 2003 2004 2003 2004 200 I 2004 2003 2004 2003 2004 2003 2004 2003 2004 2003 9004 2003 Potassium (mogA)

Feb 1.59 2.12 1.57 2.08 1.62 2.09 1.60 2.13 NS 2.29 1.57 2.06 1.59 2.15 1.57 2.16 1.60 2.12 1.46 1.98 1.46 2.14 May 1.63 1.82 1.57 1.94 NS 1.78 NS 1.90 1.58 1.83 1.59 1.75 NS 1.80 1.57 1.76 1.59 1.83 1.53 1.74 1.54 1.75 Aug 1.67 1.81 1.62 1.86 1.60 1.76 1.61 1.85 1.84 1.71 1.64 1.69 1.70 1.88 1.61 1.74 1.64 1.83 1.62 1.74 1.62 1.86 Nov 1.62 1.74 1.68 1.76 1.59 1.72 1.72 1.81 1.59 1.67 1.61 1.68 1.57 1.73 1.66 1.71 1.62 1.59 1.63 1.69 1.59 1.66 Annual Mean 1.63 1.87 1.61 1.91 1.60 1.84 1.64 1.92 1.60 1.88 1.60 1.80 1.62 1.89 1.60 1.84 1.61 1.84 1.56 1.79 1.55 1.85 Sodium (mg/I)

Feb 4.27 7.73 4.28 7.73 4.25 7.90 4.32 7.98 NS 8.26 4.24 8.25 4.22 7.84 4.25 7.73 4.22 7.89 4.43 7.00 4.39 7.58 May 4.53 5.00 4.49 5.24 NS 5.06 NS 5.78 4.61 5.02 4.59 4.57 NS 5.40 4.68 4.83 4.61 5.16 4.98 4.64 4.67 3.63 Aug 5.22 4.20 4.73 5.14 5.17 4.14 4.66 5.11 5.21 4.06 5.22 4.13 4.89 4.59 5.09 .4.16 4.75 5.10 5.28 4.14 4.77 4.90 Nov 4.62 4.03 4.89 4.17 4.61 3.98 4.81 4.50 4.63 3.96 4.60 3.94 4.62 3.94 5.19 3.94 4.49 4.10 4.08 3.94 4.07 4.09 Annual Mean 4.66 5.24 4.60 5.57 4.68 5.27 4.60 5.84 4.8-2 5.3 466- 5.22 4.58 5.44 4.80 5.17 4.52 5.56 -4.69 4.93 448 5.05 Aluminum (mgAl)

Feb 0.050 0.101 0.098 0.111 0.088 0.098 0.099 0.116 NS 0.096 0.094 0.088 0.113 0.109 0.080 0.099 0.176 0.116 0.132 0.164 0.140 0.173 May 0.050 0.286 0.050 0.442 NS 0.349 NS 0.470 0.050 0.509 0.050 0.311 NS 0.430 0.050 0.229 0.093 0.692 0.057 0.206 0.063 1.281 Aug 0.050 0.055 0.050 0.109 0.050 0.054 0.050 0.112 0.050 0.050 0.050 0.050 0.050 0.136 0.050 0.050 0.050 0.225 0.050 0.050 0.066 0.118 Nov 0.109 0.055 0.066 0.055 0.108 0.050 0.076 0.00 0100 0.020103 0.056 0.173 0.200 0.102 0.062 0.199 0.194 0.122 0.053 0.066 0.366 AnulMen 006 .7 0.066 0.17-9 0.08-2 0.138 0.075- 0.187 0.067 0.177 0.074 0.126 0.112 0.219 0.071 0.110 0.130 0.307 0.090 0.118 0.064 0.485 Iron (mg/1)

Feb 0.088 0.108 0.150 0.125 0.106 0.110 0.127 0.134 NS 0.107 0.106 0.102 0.149 0.121 0.087 0.105 0.240 0.130 0.149 0.193 0.151 0.218 May 0.059 0.320 0.061 0.450 NS 0.365 NS 0.456 0.060 0.495 0.045 0.324 NS 0.450 0.040 0.256 0.141 0.683 0.080 0.243 0.100 1.106 Aug 0.044 0.062 0.051 0.116 0.037 0.061 0.046 0.120 0.031 0.062 0.030 0.081 0.625 0.732 0.043 0.059 0.046 0.307 0.088 0.048 0.046 0.167 Nov 0.126 0.089 0.055 0.218 0.120 0.082 0.072 1.493 0.131 0.09 0.107 0.091 0.206 0.413 0.132 0.080 0.291 0.471 0.162 0.075 0.079 0.583 Annual Mean 0.079 0.1_45 0.79 0.227 -0.08-8 0.155 0.082 -0.551 0.-07-4 0.189 0.072 0.150 0.327 0.429 0.076 0.125 0.180 0.398 0.120 0.140 0.094 0.519 Manganese (ugAl)

Feb 14 10 22 10 14 10 19 13 NS 10 14 10 22 13 11 8 22 10 20 14 21 18 May 12 7 24 19 NS 11 NS 20 8 13 7 111 NS 28 6 6 30 23 11 6 21 47 Aug 23 17 481 441 24 24 245 505 34 48 30 34 1906 1191 14 21 549 1379 108 18 663 1219 Nov 117 53 8694 2251 94 60 8500 4719 262 90 125 65 438 838 60 41 985 499 55 28 264 315 Annual Mean 42 22 2305 680 44 26 2922 1314 101 40 44 30 789 518 23 19 396 478 48 16 247 400 Cadmium (ugA)

Feb NS NS NS NS 0.5 0.5 0.5 0.5 NS 0.5 NS NS NS NS 0.5 0.5 0.5 0.5 NS NS NS NS May NS NS NS NS NS NS NS NS 0.5 NS NS NS NS NS 0.5 NS 0.5 NS NS NS NS NS Aug NS NS NS NS 0.5 0.5 0.5 0.5 0.5 0.5 NS NS NS NS 0.5 0.5 0.5 0.5 NS NS NS NS Nov NS NS NS NS 0.5 NS 0.5 NS 0.5 NS NS NS NS NS 0.5 NS 0.5 NS NS NS NS NS Annual Mean NA NA NA NA 0.5 0.5 0.5 0.5 0.5 0.5 NA NA NA NA 0.5 0.5 0.5 0.5 NA NA NA NA Copper (ugA)

Feb NS NS NS NS 2.3 2.0 2.4 2.0 NS 2.0 NS NS NS NS 2.0 2.0 2.4 2.0 NS NS NS NS May NS NS NS NS 2.6 2.9 NS 2.5 2.6 13.8 NS NS NS NS 2.8 2.2 2.6 2.2 NS NS NS NS Aug NS NS NS NS 2.3 2.4 2.1 2.3 2.4 2.7 NS NS NS NS 2.6 2.6 2.0 2.7 NS NS NS NS Nov NS NS NS NS 2.0 2.0o 2.0 2.0 2.0 2.0 NS NS NS NS 2.1 2.0 2.0 2.1 NS NS NS NS Annual Mean NA NA NA NA 2.3 2.3 2.2 2.2 2.3 5.1 NA NA NA NA 2.4 2.2 2.3 2.3 NA NA NA NA Lead (ug/1)

Feb NS NS NS NS 2.0 2.0 2.0 2.0 NS 2.0 NS NS NS NS 2.0 2.3 2.0 2.0 NS NS NS NS May NS NS NS NS 2.0 2.0 NS 2.0 2.0 2.0 NS NS NS NS 2.0 2.0 2.0 2.0 NS NS NS NS Aug NS NS NS NS 2.0 2.0 2.0 2.0 2.0 2.0 NS NS NS NS 2.0 2.0 2.0 2.0 NS NS NS NS Nov NS NS NS NS 2.0 2.0 2.0 2.0 2.0 2.0 NS NS NS NS 2.0 2.0 2.0 2.0 NS NS NS NS Annual Mean NA NA NA NA 2.0 2.0 2.0 2.01 2.0 2.0 NA NA NA NA 2.0 2.1 2.0 2.0 NA NA NA NA NS = Not Sampled; NA= Not Applicable

Tl-- 'I" C Table 2-5. (Continued)

Mixing Zone Mixing Zone MNSDischarge Mixing Zone Background Background LOCATION: 1.0 Mi 2 4.0 5.0 8.0 11.0 DEPTH: Surface Bottom Surface Bottom Surface Surface Bottom Surface Bottom Surface Bottom PARAMETERS YEAR: 2004 2003 2004 2003 2004 2003 2004 2003 2004 2002 2004 2003 2004 2003 2004 2003 2004 2003 2004 2003 2004 2003 rv.~ s - - vvs-- Evv - .- - -- - - -- - - - - - .- - -- ] - -- - - --

Zinc(ugO)

Feb 20 20 20 20 20 25 30 20 NS 2 20 20 20 20 20 20 20 2 20 20 20 20 May 20 20 20 20 NS 20 NS 20 20 20 20 20 NS 20 20 20 20 2 20 20 20 20 Aug 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 2 20 20 20 20 Nov 20 20 20 20 20 20 20 20 20 20 20 20 20 20 27 20 20 2 20 20 20 20 Annual Mean 20.0 20.0 20.0 20.0 20.0 21.3 23.3 20.0 20.0 20.( 20.0 20.0 20.0 20.( 21.8 20.0 20.0 201 20.0 20.0 20.0 20.0 Nitrite-Nitrate (ug1)

Feb 200 210 210 220 200 280 220 23C NS 23( 200 220 200 230 200 420 200 390 250 350 240 350 May 210 350 250 410 NS 340 NS 41C 210 37 220 340 NS 410 190 300 260 430 220 260 270 460 Aug 90 180 330 450 70 200 340 450 90 23( 110 220 340 180 40 180 340 420 100 110 310 410 Non 180 110 20 50 190 110 20 2 190 12 190 110 170 90 190 110 180 140 220 120 220 150 Annua.lMen 170.0 212.5 202 282. 153. 2325 193.3 277. 163.3 237. 180.0 222.5 236.7 227. 155.0 252.5 245.0 345.0 197.5 210.0 260.0 342.5 Ammonia (ugA)

Feb 30 20 50 30 40 20 40 30 NS 20 40 20 40 20 40 30 30 30 20 30 30 30 May 20 40 50 20 NS 40 NS 20 30 50 20 40 NS 20 20 30 70 20 30 30 70 90 Aug 20 20 30 20 20 20 20 20 20 20 20 20 90 130 20 20 20 20 20 20 20 20 Nov 80 60 540 280 70 70 570 490 70 80 70 50 100 190 50 70 140 190 90 20 110 140 Annual Mean 37.5 35.0 167.5 87.5 43.3 37.5 210.0 140.0 40.0 42.5 37.5 32.5 76.1 90.0 32.5 37.5 65.0 65.0 40.0 25.0 57.5 70.0 TotalPhosphorous (ugl)

Feb 10 10 10 10 10 10 10 10 NS 10 10 10 10 10 10 10 10 10 10 10 10 10 May 11 11 10 12 NS 11 NS 15 10 58 10 14 NS 14 10 17 10 24 12 13 10 34 Aug 7 10 5 10 8 10 6 10 11 10 5 10 8 10 5 10 10 11 5 10 6 10 Nov 5 11 5 16 5 10 7 14 7 17 5 10 7 18 5 10 7 16 5 19 5 19 Annual Mean 8.3 10.5 7.5 12.0 7.7 10.3 7.7 12.3 9.3 23.8 7.5 11.0 8.3 13.0 7.5 11.8 9.3 15.3 8.0 13.0 7.8 18.3 Orthophosphate (ugIl)

Feb 5 5 5 5 5 2 5 l NS l 5 5 5 l 5 5 5 5 5 5 5 5 May 6 5 9 6 NS 5 NS 7 9 49 8 7 NS 31 9 9 5 5 10 17 9 5 Aug 5 5 5 l 5 5 5 l 5 5 5 5 5 5 5 5 5 5 5 5 5 Nov 5 5 5 1 5 5 13 _ 5 7 5 5 5 5 5 5 5 5 5 5 5 6 Annual Mean 53 5.0 6.0 5.3 5.0 4.3 7.7 6 6.3 17 5.8 6 5.0 12 6.0 6 5.0 5 6.3 8.0 6.0 5 Silicon(mg/l)

Feb 4.9 4.1 5.0 4.1 5.0 4.1 5.2 4.1 NS 4.1 5.0 4.1 4.8 4.1 5.0 4.1 5.0 4.1 5.1 4.5 5.1 4.4 May 4.3 3.8 4.9 4.3 NS 3.9 NS 4.3 4.4 4.0 4.3 3.9 NS 4.3 4.2 3.8 5.0 4.3 3.9 3.4 4.9 4.2 Aug 3.8 2.7 5.4 4.7 3.8 3.0 5.4 4.8 3.9 3.3 3.8 3.1 5.2 4.5 3.7 2.8 5.4 4.9 4.2 2.7 5.4 4.7 Nov 4.2 4.0 5.6 4.5 4.3 4.0 5.6 5.1 4.3 4.1 4.3 4.0 4.4 4.3 4.3 4.0 4.4 5.4 4.4 4.3 4.6 5.4 Annual Mean 43 3.7 5.2 4.4 4.4 3.8 5.4 4.6 4.2 3.9 4.4 3.8 4.8 4.3 4.3 37 5.0 4.7 4.4 3.7 5.0 4.7 NS =Not Sampled; NA=NotApplicable t'J)

80 72 69 Kibreters 0 2 4 1

.5 1.0 M1S 4.0 Figure 2- .Water quality sampling locations (numbered) for Lake Norman. Approximate locations of Marshall Steam Station (MSS), and McGuire Nuclear Station (MNS) are also shown.

Cc-2 2-25

60 55-50 45__ _

35

~30-25 15 10 5

0 1975 1977 1979 1981 1983 1985 1987 1989 1991 1993 1995 1997 1999 2001 2003 Year Figure 2-2a. Annual precipitation totals in the vicinity of McGuire Nuclear Station (MNS).

12 MNS Monthly Precipitation Totals 10 8

4 2

0-JAN FEB MAR APR MAY JUN JUL AUG SEP OCT NOV DEC Figure 2-2b. Monthly precipitation totals in the vicinity of McGuire Nuclear Station (MNS) 2-26

C le C

30 28 26 24 22

, 20 u 18 16 14

.$ 12 10 8

6 4

2 0

Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 0- Long-term average - i- 2003 -- 2004 Figure 2-2c. Mean monthly air temperatures recorded at MINS beginning in 1989. Data are complied from average daily temperatures which, in turn, were created from hourly measurements.

C C NPR Thipue 23(Q Te¶ 2 33e(q 0 5 10 15 23 3) 33 35 0 5 10 15 23 25 30 35 0 5 10 15 23 25 30 35 IV 1I It1 5- I1 I

10- I It1 S15 1 23 0APR NAY JUE TOeYPwati(q 0 5 10 15 2D 25 30 35 0 5 10 15 2D 25 30 35 0 5 10 15 20 25 30 35 F15 1.20 00 of Figure 2-3. Monthly mean temperature profiles for the McGuire Nuclear Station background zone in 2004 (xx) and 2003 (* *).

k JULY AUGUST SEPT Temperature (C) Temperature (C) Temperature (C) 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 0

5 10 o 20 25 30 35 OCT NOV DEC Temperature (C) Temperature (C) Temperature {C) 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 0 .. I...1..1

. ,t. p......1. I 2S 5

10 Ei 15 uS

- 20 MI 252 25 ' x 2X 30 1 35 Figure 2-3. (Continued).

'7 I-(

F0B NPR 0 5 10 15 23 25 30 3 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 APR NAY JUE 0 5 10 15 23 25 30 35 0 5 10 15 23 25 30 35 0 5 10 15 23 25 30 3 0 Figure 2-4. Monthly mean temperature profiles for the McGuire Nuclear Station mixing zone in 2004 (xx) and 2003 (* *).

( Ce f JULY AUGUST SEPT Temperature (C) Temperature (C) Temperature (C) 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 E 15

. 20 OCT NOV DEC Temperature (C) Temperature (C) Temperature (C) 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 0 0 5 5 10 10 10 E15- i 15

- 20 .20

- 20 a 25 25 25 30 - 30 30 35 35 35 Figure 2-4. (Continued).

45 -

40 -

35 -

"> 30-M 25 -

By 20 -

F 10 -

5-A 0 I I I I I I l Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Month 12 -

11 -

10 -

9-8-

6 ..-0-*

... 0O .

to a._)

Up 3 -

2-1-

0 Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Month Figure 2-5. Monthly surface (0.3m) temperature and dissolved oxygen data at the discharge location (loc. 4.0) in 2004 (0) and 2003 (X). September 2003 data are missing.

2-32

C r 0M 0 2 4 6 8 10 12 0 2 4 6 8 10 12 0 2 4 6 8 10 12 vnF .. I I -. r . .. I. .

5 10 g15-g15 .S152 2-

- V23 kI II 30 It

-n hmL MOY JUE DssdedOm(nv4 (diedOWM(ffqI4 0 2 4 6 8 10 12 0 2 4 6 8 10 12 0 2 4 6 8 10 12 Figure 2-6. Monthly mean dissolved oxygen profiles for the McGuire Nuclear Station background zone in 2004 (xx) and 2003 (* *).

C f JULY AUGUST SEPT Dissolved Oxygen (mg/L) Dissolved Oxygen (mgIL) Dissolved Oxygen (mg/L) 0 2 4 6 8 10 12 0 2 4 6 8 10 12 0 2 4 6 8 10 12 0

5 10 10 E 15 'i 15 a 20 a 20 a

25 25 30 30 35 35 OCT NOV DEC Dissolved Oxygen (mg/L) Dissolved Oxygen (mg/L) Dissolved Oxygen (mg/L) 0 2 4 6 8 10 12 0 2 4 6 8 10 12 0 2 4 6 8 10 12 0 , _

I 1, . . . I . . . I . a, . 1, Is 5-10 -

3lS5

.,20 a

25 -

xI I1 30 xX1 x,

35 .

Figure 2-6. (Continued).

f .

(

JAN NMR D9dIAdOq(ng¶4 D9dwedO~gn~nV¶4 0 2 4 6 8 10 12 0 2 4 6 8 10 12 0 2 4 6 8 10 12 C nto r

5 5-

'CI- 10- X I

I

,.15 2- 2 It1 It a

30 J __

AM RMV 0 42 6e 8( 10 12 d4edO6 (f8V1 1 2 0 2 4 6 8 10 12 0 2 4 6 8 10 12 0 2 4 6 8 10 12 nj Figure 2-7. Monthly mean dissolved oxygen profiles for the McGuire Nuclear Station mixing zone in 2004 (xx) and 2003 (. *).

t/2

re r

(

JULY AUGUST SEPT Dissolved Oxygen (mgIL) Dissolved Oxygen (mgIL) Dissolved Oxygen (mgIL) 0 2 4 6 8 10 12 0 2 4 6 8 10 12 0 2 4 6 8 10 12 U I.1 x.'

t k

5 A1(

.I.

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0 20 25 30 3!

OCT NOV DEC Dissolved Oxygen (mgtL) Dissolved Oxygen (mgIL) Dissolved Oxygen (mg/L) 0 2 4 6 8 10 12 0 2 4 6 8 10 12 0 2 4 6 8 10 12 0 0 . , ,,,

5 5 IC 10 10 315 2 15

a. 0.

020 la 25 30 30 0

~is 35 Figure 2-7. (Continued).

21 - ( 24 Sampling Locations 235 10o 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 23 1o I 23E l v / / 230 ,

22 co 225 200- C220-E E F5 215- 8215 w020 210 205- 205 20-Temperature (deg C) 20 Jan 7, 2004 195 . . . . . . . . . . .... ....

.195 . ..

0 5 10 15 20 25 30 35 40 45 50 55 0 5 Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km) 240- 240 Sampling Locations 235 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 235 1.0 I 23(a 230 225- 225 E 220 lE)c5o72 220 c 215 III 21O 205, 205 20 Temperature (deg C) 20

... Mar 8, 2004 I 1§,. ...,,,,...,

....,....,~O....,A .... ,dO... .....l. *-

. 10 15 20 2530 35 40 45 50 55A 5 Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km) a Figure 2-8. Monthly reservoir-wide temperature isotherms for Lake Norman in 2004.

r 24x Sampling Locations C 24U.

Sampling Locations v

r 23 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 235- 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 230-< =q 230- 28' -'lf l 225- 25 E

A 220-a 00 ED 215-i-210-205-200- Temperature (deg C)

May 5, 2004 Jun 8, 2004 I195-0 5 10 15 20 25 30 35 40 45 50 55 1 15 A 2'0 25 3A0 3`5 40 45 50 55 Distance from Cowans Ford Dam (k-) Distance from Cowans Ford Dam (km)

E E

0 4,

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Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km)

Figure 2-8. (Continued).

w 00

240 240 Sampling Locations Sampling Locations 23 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 235 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 oc21 == 210; 4X 200- 2 2052 2052 20-<Temperature (deg C) 20 - Temperature (deg C)

.Sep 13, 2004 'Oct 6, 2004 195 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I . . . . . . . ..9 I2 II 45 II 821 I 510 15 20 25 30 35 410 5`0 5 510 I1 15 20 ... 25.. 30 A35. 4`0.. 45 50 55' Distance from Cowans Ford Dam (km) Distance from Cowans Ford Darn (km) 240- 24 Sampling Locations Sampling Locations 235- 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 235- 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 21 /21 20 20--

9 /Temperature (deg C) 200 205 A Temperature 4b I (deg C)

-13v,2004 Sep_ Oct--6ec,2004 0 5 10 15 20 25 30 35 40 45 50 55 0 5 10 15 20 25 30 35 40 45 50 55 Distance from Cowans Ford Dam (kin) Distance from Cowans Ford Dam (kin) 20 Temperature 240n Sampling LocaTions tre(egC Nov84 2004 De4 4 2004 19 . .. . .. ... .. .. . . . 0 .235 . ..

-~ ..- i85I 0 5 A0 IS A .. .A A 40 451 5 111 1'5 A 5 3 ... A ... 410 ... 422 DistncefroDa (k)

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240 23 23 22 E

i22 E

5 21 jn A 21 20 195 Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km)

24. , 240-Sampling Locations Sampling Locations 235- 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 235- 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 I I 4 4 1 1 4 4 4 1
  • 4 4 1 14 230- 230-

_ 225- 225- 9 /

E 220- f220-E 8 215- 8 215-

.9 2 210- ii 210-205- 205-200 Dissolved Oxygen (mg/L) 200- Dissolved Oxygen (mg/L)

Mar 8, 2004 Apr 5, 2004 195- .. . . .. . . .. ... ... ... ... ... 1951. . .*w**w*l**lWEw 0 5 10 15 20 2135 40 45 50 5 0 5 10 )15 20 25 30 35 40 45 50 55 Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km)

, Figure 2-9. Monthly reservoir-wide dissolved oxygen isopleths for Lake Norman in 2004.

0

Sampling Locations 23 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 4 I 4 1 1 1 1 4 4f 1 230 228/

E E E

20 LU 21 0

. / May 5, 2004 45 10 l5 20 25 30 35 40 45 50 5I5 Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km) 240-Sampling Locations 235- 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 4.

I I. .

l_ .4 . . . 4. 4.

2305 22&e E E 220 Ea 5215 21 Li.

19) ) 0 1 0 5 3 5 40 4 0 5 205 20 Dissolved Oxygen (mgfL) 19 Jul 6, 2004 0 5 10 1'5 '20 25.. 3'0' -35' '40 .. 415 510 55.

Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km)

, Figure 2-9. (Continued).

C E

0 m 22 ac

'E S 21 5 21 20 200- )/

200 SSer 195- .., . . .. .. . . . . 195 5 10 15 20 25 30 35 40 C Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km) 240 24 Sampling Locations Sampling Locations 2350 t. 8.0 11.0 13.0 15.0 15.9D s62.0v 1.0 8.0 11.0 la0 15.0 15.9 62.0 689.0 72.0 80.0 1 I I I I 23

_ 22 S 22 E

SA 195.

23 10 5. 110o3. 15 .0012.93 62.0 4~

i5 21 0 5 10 15 20 25 30 35 4 20 Figre21 9 Cntne) Distance from Cowans Ford Dam (ki) Dissolved Oxygen (mg/L) 20- 20 Dec 8, 2004 1Cw> WwWwf

____.. . wwwx § l wwWi i wl l w f wXw;. wwww f-T ri I .

0 5 10 15 20 25 30 35 40 45 50 55 Distance from Cowans Ford Dam (km)

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0- II I I rI 0 50 100 150 200 250 300 350 Julian Date Figure 2-1 Oa. Heat content of the entire water column (E) and the hypolimnion (o) in Lake Norman in 2004.

12 -_ 100 90 10-80 58- 70 8 60 e 6- 50 C/i 40 M 64 30 g 20 2 -

10 0- 0 1 30 59 88 117 146 175 204 233 262 291 320 349 Julian Date Figure 2-lOb. Dissolved oxygen content (-) and percent saturation (---) of the entire water column (-) and the hypolimnion (o) of Lake Norman in 2004.

2-43

.f 24240 LAKE NORMAN STRIPED BASS HABITAT 235- 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 23 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 230- 23 2Z= 22 2205: 21 _ 210-  :

.9, 20-/Jn8260 200- / u 28,2004 20&: 205 20 Jun 8, 2004 20 Jun 28, 2004 19 . .. . .195 0 5 10 15 20 25 3`0 35 40 45 50 55 0 5 10 15 20 25 3`0 35 40 45 Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km) 24 240

-LAKE NORMAN STRIPED BASS HABITAT LAKE NORMAN STRIPED BASS HABITAT 23 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 23 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.C 4 4 4 4 4 230 23 225- _- 225-=i_=_-

E E 6deg C E 26 deg 2mg/L 21mg/L 205 205_~

200- / Jul 6, 2004 20 Ju112,2004 19 . . . . . . . . 1 0 5 10 .15 2`0 25 30 35 40 45 50 55 0 5 10 1.5 2`0 2.5 310 35 40 45 Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km)

- Figure 2-1 1. Striped bass habitat (temperatures < 26 °C and dissolved oxygen > 2 mg/L) in Lake Norman, summer 2004.

i-240-LAKE NORMAN STRIPED BASS HABITAT 235 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0

. 4 4 4 1 4 4 4 4 230-225 E

E 220 v

,E 215 / 26deg C 11 . / 2 mg/L .0 In I 20 d, Jul 19, 2004 19 1 0 5 10 15 20 25 30 35 40 45 50 5f; 5

Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km) 240 1

LAKE NORMAN STRIPED BASS HABITAT LAKE NORMAN STRIPED BASS HABITAT 235- 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 235- 1.0 8.0 11.0 13.0 15.0 15.9 62.0 69.0 72.0 80.0 I II4 4 44

4. . . .

230- 230-

- 225- - 225-220- I 220-E Aug, 20eg C E  : 0 0 / 26 deg C S 215- f 215-

/ 2 mg/L / 2 mg/L In 210- m 210-205- 205-200- _/ Aug 30, 2004 200-

/ Sep 13, 2004 195 q- I. ................................... - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

5 . 10 . 1'5 . 25 30 35 40 45--- 0 5 5 10 15 20 25 30 35 ... 40 45 50 .55 Distance from Cowans Ford Dam (km) Distance from Cowans Ford Dam (km)

Vt Figure 2-1 1. (Continued).

( (

232.0 Full Pond @ 231.65 mmsl 231.5 231.0 I

0

-J 230.5 C) 0

-J 230.0 229.5 229.0 o N 0

N N N N N N N N N'J N N 0~) 0~) C ) 0~ 0) C

0) C) C0) 0) t') C " .J 0 U)

C 0 0 40 U CD N Q CD 0 C _- C 0 .- - - N N _ O 0- - 0 N N N N N uz~~~~~~~~ _&0teOs0Oo -D - wo_

S Figure 2-12. Lake Norman lake levels, expressed in meters above mean sea level (mmsl) for 2002, 2003 and 2004. Lake level data cl correspond to the water quality sampling dates over this time period.

CHAPTER 3 PHYTOPLANKTON INTRODUCTION Phytoplankton standing crop parameters were monitored in 2004 in accordance with the NPDES permit for McGuire Nuclear Station (MNS). The objectives of the phytoplankton section of the Lake Norman Maintenance Monitoring Program are to:

1. Describe quarterly patterns of phytoplankton standing crop and species composition throughout Lake Norman; and
2. Compare phytoplankton data collected during this study (February, May, August, and November 2004) with data collected in other years during these months.

In previous studies on Lake Norman considerable spatial and temporal variability in phytoplankton standing crops and taxonomic composition have been reported (Duke Power Company 1976, 1985; Menhinick and Jensen 1974; Rodriguez 1982). Rodriguez (1982) classified the lake as oligo-mesotrophic (low to intermediate productivity) based on phytoplankton abundance, distribution, and taxonomic composition. Past Maintenance Monitoring Program studies have confirmed this classification.

METHODS AND MATERIALS Quarterly sampling was conducted at Locations 2.0, 5.0 (Mixing Zone), 8.0, 9.5, 11.0, 13.0, 15.9, and 69.0 in Lake Norman (Figure 2-1). Duplicate grabs from 0.3, 4.0, and 8.0 m (i.e.,

the estimated euphotic zone) were taken and then composited at all but Location 69.0, where grabs were taken at 0.3, 3.0, and 6.0 m due to the depth. Sampling was conducted in February, May, August, and November 2004. Secchi depths were recorded from all sampling locations. Phytoplankton density, biovolume, and taxonomic composition were determined for samples collected at Locations 2.0, 5.0, 9.5, 11.0, and 15.9; chlorophyll a concentrations and seston dry and ash-free dry weights were determined for samples from all locations. Chlorophyll a and total phytoplankton densities and biovolumes were used in 3-1

determining phytoplankton standing crop. Field sampling and laboratory methods used for chlorophyll a, seston dry weights and population identification and enumeration were identical to those used by Rodriguez (1982). Data collected in 2004 were compared with corresponding data from quarterly monitoring beginning in August 1987.

A one-way ANOVA was performed on chlorophyll a concentrations, phytoplankton densities and seston dry and ash free dry weights by quarter. This was followed by a Duncan's Multiple Range Test to determine which location means were significantly different.

RESULTS AND DISCUSSION Standing Crop Chlorophylla Chlorophyll a concentrations (mean of two replicate composites) ranged from a low of 0.97 ug/L at Location 2.0 in May, to a high of 10.57 ug/L at Location 15.9 in November (Table 3-1, Figure 3-1). All values were below the North Carolina water quality standard of 40 ug/L (NCDENR 1991). Lake-wide mean chlorophyll concentrations during all sampling periods were within ranges of those reported in previous years (Figure 3-2). The seasonal trend in 2004 of the annual low in May, increasing to the yearly maximum in August, was also recorded from 1999 (Duke Power 2000). Based on quarterly mean chlorophyll concentrations, the trophic level of Lake Norman was in the mesotrophic (intermediate) range during February, in the oligotrophic (low) range in May, and in the mesotrophic range in August and November 2004. Nearly 47% of individual chlorophyll values were less than 4 ug/L (oligotrophic) while all of the remaining chlorophyll concentrations were between 4 and 12 ug/L (mesotrophic). No chlorophyll sample exceeded 12 ug/L (eutrophic or high range). Lake-wide quarterly mean concentrations of below 4 ug/L have been recorded on ten previous occasions, while lake-wide mean concentrations of greater than 12 ug/L were only recorded during May of 1997 and 2000 (Duke Power 2001).

During 2004 chlorophyll a concentrations showed a certain degree of spatial variability.

Maximum concentrations were observed at Location 69.0 during February, May, and August, and at Location 15.9 in November. Minimum concentrations occurred at Location 9.5 in February, Location 2.0 in May and August, and Location 8.0 in November (Table 3-2). The 3-2

trend of increasing chlorophyll concentrations from down-lake to up-lake, which had been observed during most quarters of 2000 through 2003, was apparent in varying degrees during most quarters of 2004 (Table 3-1, Figure 3-1). Locations 15.9 (up-lake, above Plant Marshall) and 69.0 (the uppermost riverine location) had significantly higher chlorophyll values than Mixing Zone locations (2.0 and 5.0) during all sample periods (Table 3-2). Flow in the riverine zone of a reservoir is subject to wide fluctuations depending, ultimately, on meteorological conditions (Thornton, et al. 1990), although influences may be moderated due to upstream dams. During periods of high flow, algal production and standing crop would be depressed, due in great part, to washout. Conversely, production and standing crop would increase during periods of low flow and high retention time. Over long periods of low flow, production and standing crop would gradually decline once more. These conditions result in the comparatively high variability in chlorophyll concentrations observed between Locations 15.9 and 69.0 throughout the year, as opposed to Locations 2.0 and 5.0 which were usually similar during each sampling period.

Average quarterly chlorophyll concentrations during the period of record (August 1987 -

November 2004) have varied considerably, resulting in moderate to wide historical ranges.

During February 2004, Locations 2.0 through 8.0, and 11.0 and 13.0 had chlorophyll concentrations in the mid range, while chlorophyll concentrations at Locations 15.9 and 69.0 were in the high range for this month. The mean chlorophyll concentration at Locations 9.5 was in the low range (Figure 3-3). Long term February peaks at Locations 2.0 through 9.5 occurred in 1996, while the long term February peak at Location 11.0 was observed in 1991.

The highest February value at location 69.0 occurred in 2001. Locations 5.0 and 8.0 had higher chlorophyll concentrations in February 2004 than in February 2003, while all other locations had lower concentrations than in February of the previous year (Duke Power 2004).

During May chlorophyll concentrations at Lake Norman locations were lower than normal, and five record low concentrations were recorded for May at Locations 2.0 through 11.0 (Figure 3-3). May 2004 chlorophyll concentrations at Locations 13.0 through 69.0 were higher than those of 2003 (Duke Power 2004). Long term May peaks at Locations 2.0 and 9.5 occurred in 1992; at Location 5.0 in 1991; at Locations 8.0, 11.0, and 13.0 in 1997; and at Location 69.0 in 2001.

August chlorophyll concentrations at Locations 5.0 through 9.5, 13.0 and 69.0 were in the mid range for that time of year, while concentrations at Locations 2.0, 11.0 and 15.9 were in the low range for August (Figure 3-4). Long term August peaks in the Mixing Zone were 3-3

observed in 1998, while year-to-year maxima at Locations 8.0 and 9.5 occurred in 1993.

Long term August peaks at Locations 11.0 and 13.0 were observed in 1991 and 1993, respectively. The highest August chlorophyll concentration from Location 15.9 was observed in 1998, while Location 69.0 experienced its long term August peak in 2001. All but Location 2.0 had higher chlorophyll concentrations in August 2004 than in August of the previous year (Duke Power 2004).

During November 2004 Locations 2.0 through 9.5 had chlorophyll concentrations in the low range for that month, while Locations 11.0 through 69.0 were in the mid range for November (Figure 3-4). Long term November peaks at Locations 5.0, 8.0, and 11.0 through 15.9 occurred in 1996, while November maxima at Locations 2.0 and 9.5 were observed in 1997.

The highest November chlorophyll concentration at location 69.0 occurred in 1991.

November 2004 chlorophyll concentrations at Locations 2.0, 11.0, and 15.9 were higher than during November 2003, while values at the other locations were lower than in November of the previous year (Duke Power 2004).

Total Abundance Density and biovolume are measurements of phytoplankton abundance. The lowest density (777 units/ml) and biovolume (145 mm3 /m3 ) during 2004 occurred at Location 9.5 in February (Table 3-3, Figure 3-1). The maximum density (7,200 units/ml) was observed at Location 15.9 in August and the highest biovolume (4,101 mm 3 /m3 ) was recorded from this same location in November. Standing crop values during 2004 were most often higher than those of 2003 (Duke Power 2004). Phytoplankton densities and biovolumes during 2004 never exceeded the NC guideline for algae blooms of 10,000 units/ml density or 5,000 mm 3 /m3 biovolume (NCDEHNR 1991). Densities and biovolumes in excess of NC guidelines were recorded in 1987, 1989, 1997, 1998, 2000, and 2003 (Duke Power Company 1988, 1990; Duke Power 1998, 1999, 2001, 2004).

Total densities at locations in the Mixing Zone during 2004 were within the same statistical ranges during all sampling periods, and location 15.9 had significantly higher densities than all other locations during 2004 (Table 3-4). During most sampling periods phytoplankton densities showed a spatial trend similar to that of chlorophyll; that is lower values at down-lake locations versus up-lake locations.

3-4

Low chlorophyll concentrations and algae standing crops in May, particularly at down-lake locations, may have been due to a combination of factors. Rainfall was well below normal during the months preceding May sampling, according to MNS rainfall data. Low rainfall and subsequent runoff would have caused a depression in nutrients available to algae further down-lake. In addition, zooplankton densities in May were the highest recorded during 2004 (Chapter 4). High zooplankton densities would have resulted in increased predation on available phytoplankton throughout the lake.

Seston Seston dry weights represent a combination of algal matter, and other organic and inorganic material. Dry weights during all but May 2004 were generally higher than those of 2003, while dry weights in May were consistently lower than in the previous year. A general pattern of increasing values from down-lake to up-lake was observed in all quarters to varying extents (Figure 3-1). Statistically, Location 69.0 had significantly higher values than other locations during all quarters of 2004 (Table 3-5). From 1995 through 1997 seston dry weights had been increasing (Duke Power 1998). Values from 1998 through 2001 represented a reversal of this trend, and were in the low range at most locations during 1999 through 2001 (Duke Power 2002). Low dry weights during these years were likely a result of prolonged drought conditions. Since 2002, dry weights have gradually increased throughout the lake.

Seston ash-free dry weights represent organic material and may reflect trends of algal standing crops. This relationship seldom held true in 2004 as evidenced by a comparison of spatial distributions of ash-free dry weights and chlorophyll concentrations (Tables 3-2 and 3-5). The only near consistent relationship was at Location 69.0 where both maximum chlorophyll concentrations and ash-free dry weights were recorded in all but November.

Location 69.0 had significantly higher ash-free dry weights than other locations in all but May, when Location 69.0 demonstrated the highest ash-free dry weight, but the value was not significantly higher than most other locations (Table 3-5). The proportions of organic material among solids during 2004 were higher than in 2003. From 1996 through 2001 there was a trend of decreasing ash-free dry weight to dry weight ratios, followed by a trend of increasing ratios through 2004, indicating higher organic contributions to total solids from 2002 through 2004 (Duke Power Company 1997; Duke Power 1998, 1999, 2000, 2001, 2002, 2003, 2004).

3-5

Secchi Depths Secchi depth is a measure of light penetration. Secchi depths were often the inverse of suspended sediment (seston dry weight), with the shallowest depths at Locations 13.0 through 69.0 and deepest from Locations 9.5 through 2.0 down-lake. Depths ranged from 0.55 m at Location 69.0 in February, to 3.37 m at Location 5.0 in May (Table 3-1). The lake-wide mean secchi depth during 2004 was higher than in 2003, and was within historical ranges for the years since measurements were first reported in 1992. The deepest lake-wide mean secchi depth was recorded for 1999 (Duke Power Company 1993, 1994, 1995, 1996, 1997; Duke Power 1998, 1999, 2000, 2001, 2002, 2003, 2004). Higher secchi depths during 2004 as compared to 2003 were likely due to less rainfall and resultant lower turbidity during 2004.

Community Composition One indication of "balanced indigenous populations" in a reservoir is the diversity, or number of taxa observed over time. Lake Norman typically supports a rich community of phytoplankton species. This was certainly true in 2004. Ten classes comprising 90 genera and 210 species, varieties, and forms of phytoplankton were identified in samples collected during 2004, as compared to 95 genera and 214 lower taxa identified in 2003 (Table 3-6).

The 2003 total represented the highest number of individual taxa recorded in any year since monitoring began in 1987 (Duke Power 2004). Thirteen taxa previously unrecorded during the Maintenance Monitoring Program were identified during 2004.

Species Composition and Seasonal Succession The phytoplankton community in Lake Norman varies both seasonally and spatially within the reservoir. In addition, considerable variation may occur between years for the same months sampled.

During February 2004, cryptophytes (Cryptophyceae) dominated densities at all locations (Table 3-7, Figures 3-5 through 3-9). During most previous years, cryptophytes, and occasionally diatoms, dominated February phytoplankton samples in Lake Norman. The most abundant cryptophyte during February 2004 was the small flagellate Rhodomonas minuta. R. minuta has been one of the most common and abundant forms observed in Lake Norman samples since monitoring began in 1987. Cryptophytes are characterized as light 3-6

limited, often found deeper in the water column, or near surface under low light conditions, which are common during winter (Lee 1989). In addition, R. minuta's small size and high surface to volume ratio would allow for more efficient nutrient uptake during periods of limited nutrient availability (Harris 1978).

In May, diatoms (Bacillariophyceae) were dominant at all locations (Figures 3-5 through 3-9). The most abundant diatom was the pennate, Fragillariacrotonensis. Diatoms have typically been the predominant forms in May samples of previous years; however, cryptophytes dominated May samples in 1988, and were co-dominants with diatoms in May 1990, 1992, 1993, and 1994 (Duke Power Company 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; Duke Power 1998, 1999, 2000, 2001, 2002, 2003, 2004).

During August 2004 green algae (Chlorophyceae) dominated densities at all locations (Figures 3-5 through 3-9). The most abundant green alga was the small desmid, Cosmarium asphearosporum var. strigosum (Table 3-7). During August periods of the Lake Norman study prior to 1999, green algae, with blue-green algae (Myxophyceae) as occasional dominants or co-dominants, were the primary constituents of summer phytoplankton assemblages, and the predominant green alga was also C. asphearosporum var. strigosum (Duke Power Company 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; Duke Power 1998, 1999). During August periods of 1999 through 2001, Lake Norman phytoplankton assemblages were dominated by diatoms, primarily the small pennate Anomoeoneis vitrea (Duke Power 2000, 2001, 2002). A. vitrea has been described as typically periphytic, and widely distributed in freshwater habitats. It was described as a major contributor to periphyton communities on natural substrates during studies conducted from 1974 through 1977 (Derwort 1982). The possible causes of this significant shift in summer taxonomic composition were discussed in earlier reports, and included deeper light penetration (the three deepest lake-wide secchi depths were recorded from 1999 through 2001), extended periods of low water due to draw-down, shifts in nutrient inputs and concentrations, and macrophyte control procedures upstream (Duke Power 2000, 2001, 2002). Whatever the cause, the phenomenon was lake-wide, and not localized near MNS or Plant Marshall; therefore, it was most likely due to a combination of environmental factors, and not station operations. Since 2002, taxonomic composition has shifted back to green algae predominance (Duke Power 2003, 2004).

During November 2004, densities at all locations were again dominated by diatoms (Figures 3-5 through 3-9). The dominant species was the pennate diatom Tabellariafenestrata(Table 3-7

3-7). During the November quarters of previous years diatoms have been dominant on most occasions, with occasional dominance by cryptophytes.

Blue-green algae, which are often implicated in nuisance blooms, were never abundant in 2004 samples. Their overall contribution to phytoplankton densities was slightly higher than in 2002; however, densities of blue-greens never exceeded 5% of totals. The highest percent composition of Myxophyceae (6.3%) during all sampling periods in 2004 occurred at Location 11.0 in February. Prior to 1991, blue-green algae were often dominant at up-lake locations during the summer (Duke Power Company 1988, 1989, 1990, 1991, 1992).

Phytoplankton index Phytoplankton indexes have been used with varying degrees of success ever since the concept was formalized by Kolkwitz and Marsson in 1902 (Hutchinson 1967). Nygaard (1949) proposed a series of indexes based on the number of species in certain taxonomic categories (Divisions, Classes, and Orders). The Myxophycean index was selected to help determine long term changes in the trophic status of Lake Norman. This index is a ratio of the number of blue-green algae taxa to desmid taxa, and was designed to reflect the "potential" trophic status as opposed to chlorophyll, which gives an "instantaneous" view of phytoplankton concentrations. The index was calculated on an annual basis for the entire lake, for each sampling period of 2004, and for each location during 2004 (Figure 3-10).

For the most part, the long term annual Myxophycean index values confirmed that Lake Norman has been primarily in the oligo-mesotrophic range since 1988 (Figure 3-10). Values were in the high, or eutrophic, range in 1989, 1990, and 1992; in the intermediate, or mesotrophic, range in 1991, 1993, 1994, 1996, 1998, 2000, and 2001; and in the low, or oligotrophic, range in 1988, 1995, 1997, 1999, 2002, and 2003. The index for 2004 fell into the oligotrophic range, and was slightly higher than in 2003. The lowest annual index value recorded during the Maintenance Monitoring Program occurred during 2002.

The highest index value among sample periods of 2004 was observed in February, and the lowest index value occurred in November (Figure 3-10). The index did not reflect seasonal chlorophyll concentrations, since the maximum lake-wide chlorophyll concentration occurred in August, with the annual minimum observed in May. The index values for locations during 2004 showed a general increase from down-lake to up-lake locations. This spatial trend was similar to those observed for chlorophyll and standing crop values.

3-8

FUTURE STUDIES No changes are planned for the phytoplankton portion of the Lake Norman Maintenance Monitoring Program during 2005.

SUMMARY

In 2004 lake-wide mean chlorophyll a concentrations were generally in the mesotrophic range with the exception of May, when chlorophyll concentrations averaged in the oligotrophic range. Chlorophyll concentrations during 2004 were generally within the same ranges as those of 2003. Lake Norman continues to be classified as oligo-mesotrophic based on long term, annual mean chlorophyll concentrations. Lake-wide mean chlorophyll declined from February to the annual minimum in May, increased to the yearly maximum in August, then declined slightly in November. Some spatial variability was observed in 2004; however, maximum chlorophyll concentrations were most often observed up-lake, while comparatively low chlorophyll concentrations were recorded from Mixing Zone and mid lake locations. Location 69.0, the location furthest upstream, demonstrated maximum chlorophyll concentrations in all but November of 2004, when Location 15.9 had the highest concentration. The highest chlorophyll value recorded in 2004, 10.57 ug/L, was well below the NC State Water Quality standard of 40 ug/L.

In most cases, total phytoplankton densities and biovolumes observed in 2004 were higher than those observed during 2003, and standing crops were within ranges established over previous years. Phytoplankton densities and biovolumes during 2004 never exceeded the NC guidelines for algae blooms. Standing crop values in excess of bloom guidelines have been recorded during six previous years of the Program. As in past years, high standing crops were usually observed at up-lake locations; while comparatively low values were noted down-lake.

Seston dry and ash-free weights were more often higher in 2004 than in 2003, and down-lake to up-lake differences were apparent most of the time. Maximum dry and ash-free weights were always observed at Location 69.0. Minimum values were most often noted at Locations 2.0 through 8.0. The proportions of ash-free dry weights to dry weights in 2004 were higher than those of 2003, indicating an increase in organic composition among 2004 samples.

3-9

Secchi depths reflected suspended solids, with shallow depths related to high dry weights.

The lake-wide mean secchi depth in 2004 was slightly higher than in 2003 and was within historical ranges recorded since 1992.

Diversity, or numbers of taxa, of phytoplankton had decreased since 2003, when the total number of individual taxa was the highest yet recorded. The taxononic composition of phytoplankton communities during 2004 was similar to those of many previous years.

Cryptophytes were dominant in February, while diatoms were dominant during May and November. Green algae dominated phytoplankton assemblages during August. Blue-green algae were slightly more abundant during 2004 than during 2003; however, their contribution to total densities rarely exceeded 5 %.

The most abundant alga, on an annual basis, was the cryptophyte Rhodomonas minuta.

Common and abundant diatoms were Fragillariacrotonensis in May and Tabellaria fenestrata in November. The small desmid, Cosmarium asphearosporumvar. strigosum was dominant in August 2004. All of these taxa have been common and abundant throughout the Maintenance Monitoring Program.

The phytoplankton index (Myxophycean) characterized Lake Norman as oligotrophic during 2004, and was slightly higher than the annual index for 2003. Quarterly index values decreased from the highest in February to the lowest in November. Quarterly values did not reflect maximum and minimum chlorophyll concentrations and phytoplankton standing crops. Location index values tended to reflect increases in chlorophyll and phytoplankton standing crops from down-lake to mid-lake.

Lake Norman continues to support highly variable and diverse phytoplankton communities.

No obvious short term or long term impacts of station operations were observed.

LITERATURE CITED Derwort, J. E. 1982. Periphyton, p 279-314. In J. E. Hogan and W. D. Adair (ed.). Lake Norman Summary, vol. II. Duke power Company, Technical Report DUKE PWR/82-

02. Duke Power Company, Production Support Department, Production Environmental Services, Huntersville, NC.

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Duke Power Company. 1976. McGuire Nuclear Station, Units 1 and 2, Environmental Report, Operating License Stage. 6th rev. Volume 2. Duke Power Company, Charlotte, NC.

Duke Power Company. 1985. McGuire Nuclear Station, 316(a) Demonstration. Duke Power Company, Charlotte, NC.

Duke Power Company. 1988. Lake Norman Maintenance monitoring program: 1987 Summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1989. Lake Norman maintenance monitoring program: 1988 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1990. Lake Norman maintenance monitoring program: 1989 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1991. Lake Norman maintenance monitoring program: 1990 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1992. Lake Norman maintenance monitoring program: 1991 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1993. Lake Norman maintenance monitoring program: 1992 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1994. Lake Norman maintenance monitoring program: 1993 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1995. Lake Norman maintenance monitoring program: 1994 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1996. Lake Norman maintenance monitoring program: 1995 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1997. Lake Norman maintenance monitoring program: 1996 summary. Duke Power Company, Charlotte, NC.

Duke Power. 1998. Lake Norman maintenance monitoring program: 1997 summary. Duke Energy Corporation, Charlotte, NC.

Duke Power. 1999. Lake Norman maintenance monitoring program: 1998 summary. Duke Energy Corporation, Charlotte, NC.

Duke Power. 2000. Lake Norman maintenance monitoring program: 1999 summary. Duke Energy Corporation, Charlotte, NC.

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Duke Power. 2001. Lake Norman maintenance monitoring program: 2000 summary. Duke Energy Corporation, Charlotte, NC.

Duke Power. 2002. Lake Norman maintenance monitoring program: 2001 summary. Duke Energy Corporation, Charlotte, NC.

Duke Power. 2003. Lake Norman maintenance monitoring program: 2002 summary. Duke Energy Corporation, Charlotte, NC.

Duke Power. 2004. Lake Norman maintenance monitoring program: 2002 summary. Duke Energy Corporation, Charlotte, NC.

Harris, G. P. 1978. Photosynthesis, productivity and growth: the physiological ecology of phytoplankton. Arch. Hydrobiol. Beih. Ergeb. Limnol. 10: 1-171.

Hutchinson, G. E. 1967. A Treatise on Limnology, Vol. II. Introduction to the limonplankton. John Wiley and Sons, New York, NY.

Lee, R. E. 1989. Phycology (2nd. Ed.). Cambridge University Press. 40 West 20th. St., New York, NY.

Menhinick, E. F. and L. D. Jensen. 1974. Plankton populations, p. 120-138 In L. D. Jensen (ed.). Environmental responses to thermal discharges from Marshall Steam Station, Lake Norman, NC. Electric Power Research Institute, Cooling Water Discharge Project (RP-49) Report No. 11. Johns Hopkins Univ., Baltimore MD.

North Carolina Department of Environment, and Natural Resources, Division of Environmental Management (DEM), Water Quality Section. 1991. 1990 Algal Bloom Report.

Nygaard, G. 1949. Hydrological studies of some Danish pond and lakes II. K. danske Vilensk. Selsk. Biol. Skr.

Rodriguez, M. S. 1982. Phytoplankton, p. 154-260 In J. E. Hogan and W. D. Adair (eds.).

Lake Norman summary. Technical Report DUKEPWR/82-02 Duke Power Company, Charlotte, NC.

Thornton, K. W., B. L. Kimmel, F. E. Payne. 1990. Reservoir Limnology. John Wiley and Sons, Inc. N. Y.

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Table 3-1. Mean chlorophyll a concentrations (ug/L) in composite samples and secchi depths (m) observed in Lake Norman, NC, in 2004.

Chlorophyll a Location FEB MAY AUG NOV 2.0 3.16 0.97 3.79 3.12 5.0 3.56 1.07 5.86 2.82 8.0 3.83 1.16 7.32 2.74 9.5 2.55 1.23 6.81 3.32 11.0 4.86 1.55 4.87 6.41 13.0 5.13 3.58 5.21 6.06 15.9 7.65 3.62 7.40 10.57 69.0 7.91 6.14 9.09 4.28 Secchi depths Location FEB MAY AUG NOV 2.0 2.30 3.00 2.10 1.42 5.0 2.45 3.37 1.90 1.42 8.0 2.72 3.00 1.70 1.58 9.5 2.78 3.32 1.51 1.80 11.0 1.80 2.31 1.55 1.66 13.0 1.79 2.10 1.33 1.42 15.9 1.80 2.31 1.95 1.48 69.0 0.55 1.36 1.20 1.19 3-13

Table 3-2. Duncan's multiple Range Test on chlorophyll a concentrations (ug/L) in Lake Norman, NC, during 2004.

February Location 9.5 2.0 5.0 8.0 11.0 13.0 15.9 69.0 Means 2.55 3.16 3.56 3.83 4.86 5.13 7.65 7.92 May Location 2.0 5.0 8.0 9.5 11.0 13.0 15.9 69.0 Means 0.97 1.07 1.16 1.23 1.55 3.58 3.62 6.14 August Location 2.0 11.0 13.0 5.0 9.5 8.0 15.9 69.0 Means 3.79 4.87 5.21 5.86 6.81 7.32 7.40 9.09 November Location 8.0 5.0 2.0 9.5 69.0 13.0 11.0 15.9 Means 2.74 2.82 3.12 3.32 4.28 6.06 6.41 10.57 3-14

Table 3-3. Total mean phytoplankton densities and biovolumes from samples collected in Lake Norman, NC, during 2004.

Density (units/ml)

Locations Month 2.0 5.0 9.5 11.0 15.9 Mean FEB 970 1094 777 1582 3041 1493 MAY 861 953 1120 1132 2004 1214 AUG 3586 3864 5506 3582 7200 4732 NOV 1342 1102 1270 2400 4110 2045 Biovolume (mm 3 /m3 )

Locations Month 2.0 5.0 9.5 11.0 15.9 Mean FEB 316 357 145 812 1637 653 MAY 477 604 692 365 1056 639 AUG 1874 2720 3290 1447 3184 2503 NOV 1228 933 1374 2327 4101 1993 3-15

Table 3-4. Duncan's multiple Range Test on phytoplankton densities (units/ml) in Lake Norman, NC, during 2004.

February Location 9.5 2.0 5.0 11.0 15.9 Means 777 970 1094 15.82 3041 May Location 2.0 5.0 9.5 11.0 15.9 Means 861 953 1120 1132 2004 August Location 11.0 2.0 5.0 9.5 15.9 Means 3582 3586 3864 5506 7200 November Location 5.0 9.5 2.0 11.0 15.9 Means 1102 1270 1342 2400 4110 3-16

Table 3-5. Duncan's multiple Range Test on dry and ash free dry weights (mg/L) in Lake Norman, NC during 2004.

DRY WEIGHT February Location 5.0 2.0 9.5 8.0 13.0 11.0 15.9 69.0 Mean 1.11 1.58 1.78 1.84 2.42 2.82 3.52 19.04 May Location 2.0 5.0 8.0 9.5 11.0 15.9 13.0 69.0 Mean 0.87 0.98 1.11 1.12 1.38 1.43 1.47 2.16 August Location 2.0 5.0 8.0 9.5 11.0 13.0 15.9 69.0 Mean 2.06 2.06 2.27 2.60 2.63 2.72 3.75 9.31 November Location 8.0 11.0 13.0 9.5 15.9 2.0 5.0 69.0 Mean 1.82 2.06 2.64 2.66 2.68 2.93 3.12 3.76 ASH FREE DRY WEIGHT February Location 5.0 9.5 13.0 2.0 11.0 8.0 15.9 69.0 Mean 0.32 0.44 0.52 0.65 0.89 1.11 2.66 5.16 May Location 2.0 5.0 9.5 8.0 15.9 11.0 13 69.0 Mean 0.32 0.43 0.49 0.51 0.55 0.68 0.78 0.84 August Location 13.0 11.0 8.0 15.9 9.5 5.0 2.0 69.0 Mean 1.13 1.25 1.47 1.55 1.58 1.60 1.75 3.6 November Location 13.0 11.0 15.9 9.5 8.0 5.0 2.0 69.0 Mean 0.56 0.62 0.78 1.26 1.32 1.60 1.75 1.80 3-17

Table 3-6. Phytoplankton taxa identified in quarterly samples collected in Lake Norman each year from 1990 to 2004.

TAXON 90 91 92 93 94 95 96 97 98 99 0o 01 02 03 04 CLASS: CHLOROPHYCEAE AcanthosphaerazachariasiLemm. X X X Actidesmium hookeri Reinsch X Actinastrum hantzchii Lagerheim X X X X X X Ankistrodesmus braunii(Naeg) Brunn X X X X X X X X X X A. convolutus Corda X A. falcatus (Corda) Ralfs X X X X X X X X X X X X X X X A. fusiformis Corda sensu Korsch. X X X X X = ===

A. nannoselene Skuja __ X A. spiralis(Turner) Lemm. X X __ X X A. spp. Corda X X Arthrodesmus convergens Ehrenberg __ X X X A. incus (Breb.) Hassall x X X X X X X A. octocornis X X X A. ralfsdi W. West _-__ -_ -_X A.subulatusKutzing X X X X x x x x A. validus v. increassalatus X A. spp. Ehrenberg X X Asterococcus limneticus G. M. Smith X X X X X X =_ = X X A. superbus (Cienk.) Scherffel X Botryococcus brauniiKutzing X X CarteriafrtzschiiTakeda = = _ X X X C. globosa Korsch X X C. spp. Diesing X = x x - = = X Characiumlimneticum Lemmerman X C. spp. Braun Chlamydomonas spp. Ehrenberg X X X X X X X X X X X X X X X Chlorella vulgaris Beyerink X Chlorogoniumeuchlorum Ehrenberg X _ X X X X C. spirale Scherffel & Pascher X X X ClosteriopsislongissimaW. & W. X X X X X X X X X X X X X X X Closterium cornu Ehrenberg X X C gracileBrebisson X C. incurvum Brebisson = = = = X X X X X X X X X XX C. tumidum Johnson X C. spp. Nitzsch X X X Coccomonas orbicularisStein X - - - X = X Coelastrum cambricum Archer X X X X x X X X X X X X X x x C. microporum Nageli X X X X X C. reticulatum (Dang.) Sinn. X == =

C. sphaericumNageli X X X _ X X X X X X X C. proboscideum Bohlin ___ X C. spp. Nageli X X _

Cosmarium angulosumv. concin. (Rab) W&W = = X C. asphaerosporumv. strigosum Nord. X X X X X X X X X X X X X X X C. contractum Kirchner X X X X X X X X X X X X C. moniliforme (Turp.) Ralfs = - X X =

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Table 3-6. (Continued). Page 2 of 1 TAXON 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 C. notabile ___ X C. phaseolus f. minor Boldt. _ _ X X X _ X C. pokornyanum (Grun.) W. & G.S. West X X C. polygonum (Nag.) Archer X X X X X X X X X X C. raciborskiiLagerheim X C. regnellii Wille X X X X X X X X X X C. regnesi Schmidle - X X X X C subreniforme Nordstedt X C tenue Archer X X X X X X X X X X C. linctum Ralfs _ X X XX X X X X X X X X C. tinctum v. subretusum Messik. I X C. tinctum v. tumidum Borge. - - = - - = - X - X X X X X X C. trilobatum v. depressum Printz X C. tumidum Borge X C. spp. Corda X X X X X Crucigeniaapiculata(Lemm.) Schmidl - - X X C. crucifera(Wolle) Collins X X _ X X X X X X X X X X C.fenestrata Schmidle X X X X C. irregularisWille X X X X = X = X X X X C. rectangularis(A. Braun) Gay __ X C. tetrapedia(Kirch.) West & West X X X X X X X X X X X X X X X Dictyospaerium ehrenbergianumNageli I X X X X D. pulchellum Wood X X X X X X X X X X X X X X Dimorphococcus spp. Braun X __

Elakatothrixgelatinosa Wille X X X X X X X X X X X X X X X ErrerellabornheimiensisConrad X X Euastrum ansatum v. dideltiforme Ducel. X E. banal (Turp.) Ehrenberg X _

E. denticulatum (Kirch.) Gay X X X X X X X X X X E. elegans Kutzing X E. spp. Ehrenberg X X X Eudorina elegans Ehrenberg X X X Franceiadroescheri(Lemm.) G. M. Sm. X X X X X X X X X X F. ovalis (France) Lemm. X X X X X X X X X F. tuberculata G. M. Smith X Gloeocystis botryoides (Kutz.) Nageli = = = = X X X G. gigas Kutzing X X X X X X X X X G. major Gerneck ex. Lemmermann X G.planktonica(West & West) Lemm. X X X X X X X X X X X X X X X G. vesciculosa Naegeli = X X X X G. spp. Nageli X X X X X GolenkiniapaucispinaWest & West X X X G. radiataChodat X X X X X X X X X X X X X X X Gonium pectorale Mueller X X G.sociale(Duj.) Warming = = = - - X - X X _I X X X Kirchneriellacontorta (Schmidle) Bohlin X X X X X X X X K elongata G.M. Smith _ =- = = - X X K.lunaris(Kirch.) Mobius X X X K lunarisv. dianae Bohlin _ _ X X X X X K. lunaris v. irregularisG.M. Smith I I X X 3-19

Table 3-6. (Continued). page 3 of 10 TAXON 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 K. obesa W. West X X X X XI K. subsolitariaG. S. West _X X X X X X X X X K. spp. Schmidle X X X X Lagerheimia ciliata (Lag.) Chodat I X L. citriformis(Snow) G. M. Smith - X -

L. Iongiseta (Lemmermann) Printz X X X L. quadriseta(Lemm.) G. M. Smith X X X L. subsalaLemmerman X X X X X X X X X X X X Mesostigma viride Lauterbome X X X X X X X X X Micractinium pusillum Fresen. X X X X X X X X X X X X X X X Monoraphidium contortum Thuret = X X X X I M pusillum Printz = X X X X I Mougeitia elegantula Whittrock I X X X X X X X X X X M.spp. Agardh - - X X X Nephrocytium agardhianumNageli - X - - = - = = = - X X X N. limneticum (G.M. Smith) G.M. Smith I _I X X X OocystisborgiiSnow I I I X X X X X

0. ellyptica W. West I X X X X
0. lacustris Chodat 1 _ X X
0. parvaWest & West X = = = I X X X X X X X X X X
0. pusilla Hansgirg X X X X X X X X X X X X X
0. pyrifonnis Prescott I X X =_ =
0. solitariaWittrock I X
0. spp. Nageli X I Pandorinacharkowiensis Kprshikov =

P. morum Bory X X X X Pediastrum biradiatum Meyen 1 P. duplex Meyen X X = X X X =X X X X X X X P. duplex v. clatheatum (A. Braun) Lag. I X P. duplex v. gracillimum West and West - = - X X - - X X X P. tetras v. tetroadon (Corda) Rabenhorst X X X X X X X X X X X X X X X P. spp. Meyen X X Planktosphaeriagelatinosa G. M. Smith X X X Quadrigulaclosterioides(Bohlin) Printz - X X X X X X X Q. lacustris(Chodat) G. M. Smith X X X Scenedesmus abundans (Kirchner) Chodat X - - - - - - - =- = - X S. abundans v. asymetrica (Schr.) G. Sm. X X X X X I X X - - X X X X S. abundans v. brevicauda G. M. Smith IX X X S. acuminatus (Lagerheim) Chodat - - X X X X X - X X X X X X X S. armatus v. bicaudatus (Gug.-Pr..)Chod X X X X X X X X X X X X X X X S. byuga (Turp.) Lagerheim X X X X X X X X X X X X X S. bijuga v. alterans(Reinsch) Hansg.1 X S. brasiliensisBohlin I I IX X X X X X X X X X S. denticulatusLagerheim X X X X X X X X X X X X X X S. denticulatus v. recurvatus Schumacher I X X S. dimorphus (Turp.) Kutzing X X X X X X X X X X X S. incrassulatusG. M. Smith 1 S. parisiensisChodat X S. quadricauda(Turp.) Brebisson X X X X X X X X X X X X X X X S. smithii Teiling - - I X _ X X =

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Table 3-6. (Continued). page 4 of 10 TAXON 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 S. serratus(Corda) Boblin - X S. spp. Meyen X X X X X Schizochlamys compacta Prescott X X X X X S. gelatinosa A. Braun X X X Schoederia setigera(Schroed.) Lemm. - X - = = - - = - X Selenastrum gracile Reinsch = X X X S. minutum (Nageli) Collins X X X XX X X X X X X X X X X S. westii G. M. Smith X X X X X X X X Sorastrum americanum (Bohlin) Schm. X _

Sphaerocystis schoeteri Chodat X - X X XX X X X Sphaerozosma granulatum Roy & Bl. 1 Stauastrum americanum (W&W) G. Sm. X X X X X X X X X X S. apiculatum Brebisson X X X X X X X X S. brachiatum Ralfs _ X X X XX X S. brevispinum Brebisson X S. chaetocerus (Schoed.) G. M. Smith X X X S. curvatum W. West X X X X X X X X X X X X X X X S. cuspidatum Brebisson X X X X X X X X S. dejectum Brebisson X X X = X == = X X S. dickeii v. maximum West & West I S. dickeii v. rhomboidium W.& G.S. West X S. gladiosum Turner X S. leptocladum v. sinuatum Wolle - X = = - = - = - = - - -

S. manfeldtii v. fluminense Schumacher X X X X X X X X X X S. megacanthum Lundell X X X X S. ophiurav. cambricum (Lund) W. & W. X S. orbiculareRalfs X X S. paradoxum Meyen X X X X X X X X S. paradoxum v. cingulum W. & W. 1 - X S. paradoxum v. parvum W. West === X X X X S. pentacerum (Wolle) G. M. Smith X S. subcruciatumCook & Wille X X X X X X X X S. tetracerumRalfs X X X X X X X X X X X X X X X S. turgescens de Not.

S. vestitum Ralfs X X S. spp. Meyen X X X Stichococcus scopulinus Hazen X Stigeoclonium spp. Kutzing X Tetraedron arthrodesmiforme(W.) Wol. X X Tetraedron bifurcatum v. minor Prescott X T. caudatum (Corda) Hansgirg X = X = X X X X X X X X X X T. limneticum Borge X T. lobulatum (Naeg.) Hansgirg = = = - X - -

T. lobulatum v. crassum Prescott =__ =__ - - - X T. minmum (Braun) Hansgirg X X X X XX X X X X X T. muticum (Braun) Hansgirg X X X X X X X T. obesum (W & W) Wille ex Brunnthaler = = X = = - = = = =

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Table 3-6. (Continued). page 5 of 10 TAXON 90 91 =92 93 94 95 96 97 98 99 00 01 02 03 04 lT. pentaedricum West & West X _- .-

T planktonicum G. M. Smith X X_ X X X T. regulareKutzing - X X X X = = = = -

T. regulare v. bifurcatum Wille - _ X T regularev. incus Teiling -_ X _ _ _

T. trigonum (Nageli) Hansgirg XX X X XX X X X X X T trigonum v. gracile (Reinsch) DeToni X X X X T. spp. Kutzing X __ X TetrallantoslagerheimiiTeiling X X X Tetraspora lamellosa Prescott X T spp. Link X X Tetrastrum heteracanthum(or.) Chod. X X X T. staurogeniforme (Schroeder) Lemm. X Treubariasetigerum (Archer) G. M. Sm. X X X X X X X X X X X X X X X Westella botryoides (W. & W.) Wilde. _ X X X W. linearis G. M. Smith X X X X Xanthidium critatatumv. uncinatum Breb. X X X spp. Ehrenberg X _ X CLASS: BACILLARIOPHYCEAE Achnanthes lanceolataBreb. X A. microcephalaKutzing X _X X X X X X X X X A. spp. Bory X X X X X IX Amphiphora ornateBailey X _

Anomoeoneis vitrea (Grnow) Ross X X X X - X X X X X X X A. SDD. Pfitzer X Asterionellaformosa Hassall X X X X X X X X X = X X X X X AttheyazachariasiJ.Brun X X X X X X X X X X X X X X Cocconeis placentulaEhrenberg XX X C. spp. Ehrenberg - - = = X =====

Cyclotella comta (Ehrenberg) Kutzing = = X X X X X X X X X X X C. glomerata Bachmann X X X X X X X C. meneghinianaKutzing X X X X X X X X X X C. pseudostelligeraHustedt 1 =

C. stelligeraCleve & Grunow X X X XI XI X X X X X X X X X X C. spp. Kutzing X_I Cymbella affinis Kutzing I__ X X C. gracilis (Rabh.) Cleve X X C. minuta (Bliesch & Rabn.) Reim. X - X X X X X X X X X C. tumida (Breb.) van Huerck X C. turgida (Gregory) Cleve 1 C. spp. Agardh X Denticulaelegans Kutzing . _ -X X D. thermalis Kutzing X X Diploneis ellyptica (Kutz.) Cleve _ X D. ovalis (Hilse) Cleve X D. puella (Schum.) Cleve X D. spp. Ehrenberg X _

Eunotia flexuosa v. eurycephala Grun. = == _-= _ _ _ =_ - -

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Table 3-6. (Continued). page 6 of 10 TAXON 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 E. zasuminensis (Cab.) Koemer X X X X X X X X X X X X X X Fragilariacrotonensis Kitton X X X X X X X X X X X X X X X F. construens =X Frustuliarhomboides (Her.) de Toni1 F. rhomboides v. saxonica (Rabh.) de T. X Gomphonema angustatum (Kutz.) Rabh. X G. parvulum Kutz. X X G. spp. Agardh X X Melosiraambigua (Grun.) 0. Muller X X X X X X X X X X X X X X X M distans (Her.) Kutzing X X X X X X X X X X X X X X X M granulata(Ehr.) Ralfs X X __ X M granulatav. angustissima0. Muller X X X X X X X X X X X X X X X M italica (Ehr.) Kutzing 1 =

M varians Agardh X X == = X M spp. Agardh X X X X X X X X X X X Meridion circulareAgardh X Navicula cryptocephalaKutzing X ___ X X - _ _ X _

N. exigua (Gregory) 0. Muller X X X N. exigua v. capitataPatrick X N. radiosaKutz. X N. radiosav. tenella (Breb.) Grun. X X N. subtilissimaCleve X = X X N. spp. Bory X X X X X X Nitzschia acicularisW. Smith X X X X X X X X X X X X X N. agnitaHustedt X X X X X X X X X X X X X X X N. holsaticaHustedt X X X X X X X X X X X N. kutzingianaHilse _X N. IinearisW. Smith _ X _

N. palea (Kutzing) W. Smith X X XX=XX X X N. sublinearisHustedt X = X X X N. spp. Hassall X X X X X X Pinnulariaspp. Ehrenberg = X X Rhizosolenia spp. Ehrenberg X X X X X X X X X X X X X X X Skeletonema potemos (Weber) Hilse X X X = X X X X X Stephanodiscus spp. Ehrenberg X X X X X X X X X X X Surirellaangustata Kutz. X S. linearis v. constricta (Ehr.) GrO. __X Synedra actinastroidesLemmerman X S. acus Kutzing X X X X = X X X X S. delicatissimaLewis X X X S.filiformis v. exilis Cleve-Euler X - X XX X X S. planktonica Ehrenberg X X X X X X X X X X X X X X X S. rumpens Kutzing X X X X X X X X X X S. rumpens v. fragilarioidesGrunow I S. rumpens v. scotica Grunow i S. ulna (Nitzsch) Ehrenberg X X X X X X X X X X S. spp. Ehrenberg X X X X X = ===

Tabellariafenestrata (Lyngb) Kutzing X X X X X X X X X X X X X X X T.flocculosa (Roth.) Kutzing  : X _ X X X 3-23

- - - r -r . * .1 ____ 7 _rrrb I able 3-6. ((ontinueci). -age a / oe 1u TAXON 90 91 92 93 94 95 96 97 98 99 oo 01 02 03 04 CLASS: CHRYSOPHYCEAE Aulomonas purdyii Lackey X X X X X X X X X X Bicoeca petiolatum (Stien) Pringsheim _ X X Calycomonas pascheri(Van Goor) Lund X X X Chromulinaspp. Chien. __. X X X X Chrysococcus rufescens Klebs X ChrysosphaerellasolitariaLauterb. X X X X X X X X X X X X X X Codomonas annulata Lackey X X X X X X X X Dinobryonbavaricum Imhof X X X X X X X X X X X X X X X D. cylindricum Imhof X X X X X X X X X D. divergens Imhof X X X X X X X X X X D. sertulariaEhrenberg X X D. spp. Ehrenberg X X X X X X X X X X X X Domatomococcus cylindricum Lackey _ X X X Erkinia subaeguicilliataSkuja X X X X X X X X X X X X Kephyrion campanuliforme Conrad X K littorale Lund _ X X X K. petasatum Conrad X K rubi-claustriConrad X X X K skujae Ettl 1 K spp. Pascher XX X X X X X X X X X X X X X Mallomonas acaroidesPerty X M akrokomos (Naumann) Krieger X X X X X M allorgii(Defl.) Conrad - X M alpina Pascher X X M caudataConrad X X X X X X X X X X X _

M globosaSchiller x _ x x x x x x M productaIwanoff ___ X X X M pseudocoronataPrescott X X X X X X X X X X X X X X X M tonsurataTeiling X X X X X X X X X X X X X X X M spp. Perty X X X X X X Ochromonas granularisDoflein _ X XXX X X X

0. mutabilis Klebs X
0. spp. Wyss X XX X X X X X X X X Pseudokephyrion schilleri Conrad = I X X X X X P. tintinabulum Conrad __ _ X P. spp. Pascher X Rhizochrisispolymorpha Naumann X X X X X X R. spp. Pascher X Salpingoecafrequentissima (Zach.) Lem. _ X X X X Stelexomonas dichotoma Lackey X X X X X X X X X - X X X Stokesiella epipyxis Pascher I I X X X I =

Synura sphagnicolaKorschikov X S. spinosa Korschikov X X X XX X X X X X X S. uvella Ehrenberg X X X X X S. spp. Ehrenberg X X X X X Uroglenopsis americana(Caulk.) Lemm. - - - X X X - X - - - = =

3-24

Table 3-6. (Continued). nage 9 of 10 Tabl 3-6(CotinudV ape 8 of 10 TAXON 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 CLASS: HAPTOPHYCEAE Chrysochromulinaparva Lackey X X X X X X X X X X X X X X X CLASS: XANTHOPHYCEAE Characiopsisacuta Pascher X C. dubia Pascher X X X X X X X X X Dichotomococcus curvata Korschikov =

Ophiocytium caoitatumv. longisp. (M) L. X X X X Stipitococcus vas Pascher X CLASS: CRYPTOPHYCEAE Cryptomonaserosa Ehrenberg X X X X X X X X X X X X X X X C. erosav. reflexa Marsson X X X X X X X X C. graciliaSkuja X C. marsoniiSkuja X X X X X X C. obovata Skuja X C. ovata Ehrenberg X X X X X X X X X X X X X X X C. phaseolus Skuja X X X X X I C. reflexa Skuja X X X X X X X X X X X X X X X C. spp. Ehrenberg X X X X X Rhodomonas minutaSkuja X X X X X X X X X X X X X X X CLASS: MYXOPHYCEAE Agmenellum quadriduplicatumBrebisson X X X X X X X X X X X X A. thermale Drouet and Daily - X -

Anabaena catenula (Kutzing) Born. - -

A. inaegualis(Kutz.) Born. __ X A. scheremetievi Elenkin XX X =_ X A. wisconsinense Prescott X X X X X X X X X X A. spp. Bory X XX X X X X = X X X Anacystis incerta (Lemm.) Druet & Daily X X X X XI X X X A. spp. Meneghini I I I Chroococcus dispersus (Keissl.) Lemm. =X X C. limneticus Lernmermann X X X X X X X X C. minor Kutzing X X C. turgidus (Kutz.) Lernmermann X X C. spp. Nageli X X X X X X X X X X X X X X CoelosphaeriumkuetzingianaNageli X _

Dactylococcopsis irregularisHansgirg X X X X X D. rupestrisHansgirg = = = = - X D. smithii Chodat and Chodat X X X X X D. spp. Hansgirg X Gomphospaerialacustris Chodat X X X X X Lyngbya contorta Lemmermann X X L. limnetica Lernmermann X X X X X L. ochracea(Kutz.) Thuret X X X L. subtilis W. West X X X X L. tenue _ _ II I I L. spp. Agardh X X X X X XXXX X X X X X I X 3-25

Table 3-6. (Continued). page 9 of 10 TAXON 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 Merismopedia tenuissima Lemimermann = = = = = = = X = = = = = =

Microcystis aeruginosaKutz. emend Elen. X X X X X X X = X X X XX Oscillatoriaamoena (Kutz.) Gomont _ X amphibia Agardh X X.X X

0. geminata Meneghini X - _ _ - X X X X X X X X X X
0. limnetica Lemmermann X X X X X X X X X X
0. splendida Greville X X X X
0. subtilissima Kutz. = ==X X X X X
0. spp. Vaucher X X X Phormidium angustissimum West & West X X X P. spp. Kutzing X = X X =

Raphidiopsiscurvata Fritsch & Rich _X X X X X I X X X X X R. mediterraneaSkuja =X -

Rhabdodermasigmoidea Schm. & Laut.1 Spirulinasubsala Oersted X I Synecococcus lineare (Sch. & Lt.) Kom. X X X X X X X - X X X X X X CLASS: EUGLENOPHYCEAE Euglenaacus Ehrenberg X X X E. minuta Prescott _X X X E. polymorpha Dangeard X X X _ X E. proxima Dangeard - - - - - _ X X E. spp. Ehrenberg X X X X X X X X = X X Lepocinclus acuta X L. glabra Drezepolski X L. ovum. (Ehr.) Lemm. X X L. spp. Perty -X I Phacus cuvicauda Swirenko _X P. longRicauda (Ehr.) Dujardin X P. orbicularisHubner X P. tortus (Lemm.) Skvortzow X - X P. triguterPlayfair X P. spp. Dujardin 1=

Trachelomonasacanthostoma (Stk.) Defl. X X T. ensifera Daday X T hispida(Perty) Stein X X X X X X X T. pulcherrimaPlayfair I T. pulcherrimav. minor X T. volvocina Ehrenberg _ X X X X-T spp. Ehrenberg X X X CLASS: DINOPHYCEAE Ceratium hirundinella(OFM) Schrank X X X X X X X X Glenodinium borgei (Lemm.) Schiller X G. gymnodinium Penard X X X X X X G. palustre (Lemm.) Schiller 1 G. penardiforme (linde.) Schiller XX __ X__

G. quadridens (Stein) Schiller X - X G. spp. (Ehrenberg) Stein - X __ X _ - -

3-26

Table 3-6. (Continued). page 10 of 10 TAXON 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 Gymnodinium aeruginosum Stein X X X X X G. spp. (Stein) Kofoid & Swezy X X X X X X X X = X X X X X Peridiniumaciculiferum Lemmermann 1 P. inconspicuum Lemmermann X X X X X X X X X X X X X X X P. cinctum (Muller) Ehrenberg X P. intermedium Playfair X X X X X X X P.pusillum (Lenard) Lemmermann X X X X X X X X X X X X X X P. umbonatum Stein = X X X P. willei (Huitfeld-Kass _ X P. wisconsinense Eddy X X X X X X X X X X X X X X X P. spp. Ehrenberg X X X X X CLASS: CHLOROMONADOPHYCEAE Gonyostomum depresseum Lauterborne X X X X X X G. semen (Ehrenberg) Diesing X _ _

G. spp. Diesing X =_=_ X 1= taxa found during 1987-89 only 3-27

Table 3-7. Dominant classes, their most abundant species, and their percent composition (in parenthesis) at Lake Norman locations during each sampling period of 2004.

LOC FEBRUARY MAY 2.0 CRYPTOPHYCEAE (66.1) BACILLARIOPHYCEAE (70.2)

Rhodomonas minuta (62.4) Fragillariacrotonensis (52.8) 5.0 CRYPTOPHYCEAE (65.9) BACILLARIOPHYCEAE (67.9)

R. minuta (58.2) F. crotonensis (48.3) 9.5 CRYPTOPHYCEAE (55.2) BACILLARIOPHYCEAE (69.9)

R. minuta (53.6) F. crotonensis (54.7) 11.0 CRYPTOPHYCEAE (45.3) BACILLARIOPHYCEAE (57.9)

R. minuta (40.3) F. crotonensis (42.1) 15.9 CRYPTOPHYCEAE (54.5) BACILLARIOPHYCEAE (53.8)

R. minumta (45.7) F. crotonensis (35.6)

AUGUST NOVEMBER 2.0 CHLOROPHYCEAE (62.5) BACILLARIOPHYCEAE (44.2)

Cosmarium asphear. strig. (39.3) Tabellaria.fenestrata (19.7) 5.0 CHLOROPHYCEAE (67.5) BACILLARIOPHYCEAE (57.5)

C. asphearosporumstrig. (40.4) T.fenestrata (34.2) 9.5 CHLOROPHYCEAE (67.2) BACILLARIOPHYCEAE (61.2)

C. asphearosporumstrig. (47.5) T. fenestrata(40.7) 11.0 CHLOROPHYCEAE (62.9) BACILLARIOPHYCEAE (49.0)

C. asphearosporumstrig. (43.3) T.fenetrata (14.3) 15.9 CHLOROPHYCEAE (65.5) BACILLARIOPHYCEAE (46.1)

C. asphearosporumstrig. (49.4) T.fenestrata (18.3) 3-28

CHLOROPHYLL a (ugl)D DENSITY (units/ml) 7000 ---------------------- I 6000 --------------------

5000 4000 3000 -------------- ---

2000 -------------

1000i - - -

2.0 L.0 9.E 11.0 15.(

2.0 5.0 8.0 9.5 11.0 13.0 15.9 69.0 SESTON DRY WEIGHT (mgI1) BIOVOLUME (mm31m3) 20 451 401 iOO--__ -_-- -- -- --

I 35t 30t 140 - - 25t 10 -------------------- /--

20t 15(

01 6

2; 10t 5S oo

)O.I

-- - - -- -2 -- -

0 2.0 5.0 8.0 9.5 11.0 13.0 15.9 69.0 2.0 5.0 9.5 11.0 15.9 LOCATIONS LOCATIONS FEB MAY AUG NOV Figure 3-1. Phytoplankton chlorophyll a, densities, biovolumes, and seston weights at locations in Lake Norman, NC, in February, May, August, and November 2004.

3-29

14 12 8-0 Z6-J 0

4 - - --

0 0 i FEB MAY AUG NOV MONTH

+ 1987 - 1988 A 1989 -- X- 1990 + 1991

-- *--1992 1-1993 - 1994 - 1995 *-->--1996

- 1997 -<-1998 & 1999 0 2000 O-2001 2002 -- e-2003 ---0 2004 Figure 3-2. Phytoplankton chlorophyll a annual lake means from all locations in Lake Nornan, NC, for each quarter since August 1987.

3-30

CHLOROPHYLLa (ug/h)

FEBRUARY MAY 2.0 -- 5.0 --2.0 5.0 30MIXING ZONE 30 4 25 - - - - - - - - - - - - - - - - - _- - _- - _- - 25 - - - - - - - - - - - - - - - - - - - -

1 20 - - - - - - - - - - - - - - - - - _- - _- - _- -

115 _--

- - -- _- -_-__-_ - 15 - - - - - - - - - - - - - - - _- - _- - _- - _--

1 I5 0 15 S

87 89 91 93 95 97 99 01 03 87 89 91 93 95 97 99 01 03 1--8.0 -_-9.5 1-8.0 +9.5 30 SuT 25 - - - - - - - - - - - - - - - - - - - - - - - . I 2 0 - - - - - - - - - - - - - - - - - - - - - - -_ I20-- - - - - - - - - - - - - - - - - - - - - -_

15 - - - - - - - - - - - - - - - - - - I15 -- _____-__-__-__--__-__-__-__-__-

5f I10 ----

8 87 89 91 93 95 97 99 01 03 87 89 91 93 95 97 99 01 03 11.0 _313.0 11.0 13.0]

30 on 25 - - - - - - - - - - - - - - - - - - - - - I 20 - - - - - - - - - - - - i- - - - - - - -

20 - - - - - - - - - - - - - - - - - - - - _ I 15 115 20 1 0o 87 89 91 93 95 97 99 01 03 87 89 91 93 95 97 99 01 03 1-415.9 69.0] 1-4-15.9 -_-69.0 87 89 91 93 95 97 99 01 03 87 89 91 93 95 97 99 01 03 YEARS YEARS Figure 3-3. Phytoplankton chlorophyll a concentrations by location for samples collected in Lake Norman, NC, from February and May 1988 through 2004.

3-31

CHLOROPHYLL a (ug/1)

AUGUST NOVEMBER 2.0 -45. --- 2.0 ----.

+--

35 - 35 MIXING ZONE 30 30 MIXING ZONE- --- --

25 25 ______________________-

20 20 15 ______________________-

110 10 5

0 0 87 89 91 93 95 97 99 01 03 87 89 91 93 95 97 99 01 03

-.- 8.o -U-9 5 4-~8.0 R-9.5 35 35 30 - - - - - - - - - - - -_ 30 - - - - - - - -_

25 - - - - - - - - - - - - - - - - -- - -

20 - - - - - - - - - - - - - - - - - - - _ 20 - - - - - - - -_

20 15 15 - - - - - - - -_

10 87 89 91 93 95 97 99 01 03 87 89 91 93 95 97 99 01 03 1-4-io --- 13.01 I--11.0 -- 13.0 35 30- -----------------------

I!O -- - - - - - - - - - - - - - - - - - -- 25 - -----------------------

20 - -----------------------

15 -- - - -- - - - - - -- - -- - - - - --

15 - -----------------------

105 I 10-I -- ---- IF IL - a,- --

0 iiii i!!i)  !

87 89 91 93 95 97 99 01 03 87 89 91 93 95 97 99 01 03 1--4S1.9 -- 69.6] 1---15.9 s -- 69.

35 35 . . .

30 -- - - - - - - - - - - - - - - - - - - - - 30 25 -- - - - - - - - - - - - - - - - - - - - 25 20 -- - - - - - - - - - 2 15 0 - --- - --

10 5I -------- --------- 0 0 I . I i i i i - i i I 87 89 91 93 95 97 99 01 03 87 89 91 93 95 97 99 01 03 YEARS YEARS Figure 3-4. Phytoplankton chlorophyll a concentrations by location for samples collected in Lake Norman, NC, from August and November 1987 through 2004.

3-32

5000 4500 4000 3500 E 3000 2500 2n Z 2000 FEB MAY AUG NOV 2800 2600 2400 2200

.^ 2000 E 1800 2800 - __-_ __ _

-_- -___ _ __ m -----------------

° 1600 1400 4-2000 - - - --- - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

1200 0-800 -

FEB MAY AUG NOV Figure 3-5. Class composition (mean density and biovolume) of phytoplankton from euphotic zone samples collected at Location 2.0 in Lake Norman, NC, during 2004.

3-33

annn oCHLOROPHYCEAE ioCHRYSOPHYCEAE 4500 .MYXOPHYCEAE g

  • OTHERS 4000 3500 E3000

=2500 Z 2000 1500 1000 500 MAY AUG NOV 3000 2800 -

2600 - -------------------------------

2400 - -------------------------------

2200 - ------------------------------- --:--------::::

- 2000 - -------------------------------

-E E 1800- -------------------------------

E ui 1600 - -------------------------------

E 1400- -------------------------------

-J o 1200- -------------------------------

0 X 1000 - ------------------------------- - -_-----

800 --------------------------------

600 400 200 ----------------

U1 MAY AUG NOV Figure 3-6. Class composition (mean density and biovolume) of phytoplankton from euphotic zone samples collected at Location 5.0 in Lake Norman, NC, during 2004.

3-34

1000 0 a3 CHLOROPHYCEAE ig BACILLARIOPHYCEAE 900 0 - m CHRYSOPHYCEAE igCRYPTOPHYCEAE oBMYXDPHYCEAE m DINOPHYCEAE 800 0 -

  • OTHERS 700 0 -

hI; 600 0 -

I

_ 500(0 - ---- - - - -- - - - ----- --- - - - -- - -

0, Z 40010 -

0Q 300( 0 -

200( -D 100( -D FEB MAY AUG NOV 5000 4500 4000 3500 _____________________________________________________

4-E

-k-____--____--____--____-- ---

E 2500

° 2000 0

1500 1000 500 0


_------ km---_---_-_

MAY AUG


NOV Figure 3-7. Class composition (mean density and biovolume) of phytoplankton from euphotic zone samples collected at Location 9.5 in Lake Norman, NC, during 2004.

3-35

6000 -- -------

>5000-Z 4000 -- - - - - - - - - - - -- -

3000- _-- --- --- _ --

2000 ------------

1000- ------ I_= ------ ---- -- --

0 FEB MAY AUG NOV 5000 4500 __________________--_________--___--_________--______

4000 ________--________--_______--_____--_____--______

3500 __________________--________________--______--______

E X 2500 ________________--______________--_________________

g 20 0 0 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - --

2 1500 __--______________--__________--______-----_

1000 __ ---

500 =--

FM MAY AUG NOV Figure 3-8. Class composition (mean density and biovolume) of phytoplankton from euphotic zone samples collected at Location 11.0 in Lake Norman, NC, during 2004 3-36

E 6000 _- --

Z 4000-- ________________

3000- -_- -__________

2000 - - -------- _ _ _ _ - -

'U 300 1000 - - - - - - -- --- -=

FEB MAY AUG NOV 5000-4500 - - - - - - _---- ------- --------- - -------111 4 000- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -_ -__

3500 - - - - -- - - -- - - - - -- - - -- - - -- - - - -- - - -- - -- - - - -- - - -  ! ! __

22500 _ __ - -- - _ _ _ _ _ _ _ _ _ . --_______- - --

-j

? 2000 - - - - - - - - - - - - - - -- - --- - -

0 1500 -- - - - - - - ----- - - - --- -

1000 - -- ----- ------ -------- ---

500 - E- - -

0-FEB MAY AUG NOV Figure 3-9. Class composition (density and biovolume) of phytoplankton from euphotic zone samples collected at Location 15.9 in Lake Norman, NC, during 2004.

3-37

MYXOPHYCEAN INDEX: LAKE NORMAN

1. .
5 - - - - - - - -- - H IG - _10 1.3 - -- - - - - - - - - - - - - - INTERMEDIATE - - - - -

1.2 - - - - -- - - -- - - -

1.1- - - -- - - - - - - - - -

0. -- - - -- - - -- - -- - - -- - - - --

ttt0'8 7 - - - - I:-;-0-i- - - - l-- - - -- - -

1988 1989 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 2003 2004 YEARS 3

z 2

0 FEB MAY AUG NOV MONTH 15 - _

0.1 0.1 01(

0.E70 - --_ -------- ------------------- ----- ----- -----

0.!

0.

0.4 0A410 55 -

_n0_ _______________________

2 5 9.5 11 15.9 LOCATIONS Figure 3-1 0. Myxophycean index values by year (top), each quarter in 2004 (mid), and each location in Lake Norman, NC, during 2004.

3-38

CHAPTER 4 ZOOPLANKTON INTRODUCTION The objectives of the Lake Norman Maintenance Monitoring Program for zooplankton are to:

1. Describe and characterize quarterly patterns of zooplankton standing crops at selected locations on Lake Norman; and
2. compare and evaluate zooplankton data collected during this study (February, May, August, and November 2004) with historical data collected during the period 1987-2003.

Previous studies of Lake Norman zooplankton populations, using monthly data, have demonstrated a bimodal seasonal distribution with highest values generally occurring in the spring, and a less pronounced fall peak. Considerable spatial and year-to-year variability has been observed in zooplankton abundance in Lake Norman (Duke Power 1976, 1985; Hamme 1982; Menhinick and Jensen 1974). Since quarterly sampling was initiated in August 1987, clear cut bimodal seasonal distribution has been less apparent due to lack of transitional data between quarters.

METHODS AND MATERIALS Duplicate 10 m to surface and bottom to surface net tows were taken at Locations 2.0, 5.0, 9.5, 11.0, and 15.9 in Lake Norman (Chapter 2, Figure 2-1) in February, May, August, and November 2004. For discussion purposes the 10 m to surface tow samples are called epilimnetic samples and the bottom to surface net tow samples are called whole column samples. Locations 2.0 and 5.0 are defined as the Mixing Zone and Locations 9.5, 11.0 and 15.9 are defined as Background Locations. Field and laboratory methods for zooplankton standing crop analysis were the same as those reported in Hamme (1982). Zooplankton standing crop data from 2004 were compared with corresponding data from quarterly monitoring begun in August 1987.

4-1

A one way ANOVA was performed on epilimnetic total zooplankton densities by quarter.

This was followed by a Duncan's Multiple Range Test to determine which location means were significantly different.

RESULTS AND DISCUSSION Total Abundance During 2004, typical seasonal variability was observed in epilimnetic samples. Maximum epilimnetic densities were observed in May at Locations 2.0, 5.0, and 15.9 and in February at Locations 9.5 and 11.0 (Table 4-1, Figure 4-1). The lowest epilimnetic densities occurred in August at all but Location 15.9 which had its lowest annual density in February. Epilimnetic densities ranged from a low of 29,413/m 3 at Location 2.0 in August, to a high of 359,434/m3 at Location 15.9 in May. Maximum densities in the whole column samples were also observed in May at Locations 2.0, 5.0 and 15.9, while maxima at Locations 9.5 and 11.0 were recorded in February. Minimum whole column densities were observed at all Locations in August. Whole column densities ranged from 19,381/m 3 at Location 2.0 in August, to 186,346/m3 at Location 15.9 in May.

Historically, maximum epilimnetic zooplankton densities at Lake Norman locations have most often been observed in May, with annual peaks observed in February about 25% of the time. Annual maxima have only occasionally been recorded for August and November (Duke Power 2004).

Total zooplankton densities were consistently higher in epilimnetic samples than in whole column samples during 2004, as has been the case in previous years (Duke Power 2004).

This is related to the ability of zooplankton to orient vertically in the water column in response to physical and chemical gradients and the distribution of food sources, primarily phytoplankton, which are generally most abundant in the euphotic zone (Hutchinson 1967).

Although spatial distribution varied among locations from season to season, a general pattern of lower average densities from the Mixing Zone as compared to Background Locations was observed during 2004 (Tables 4-1 and 4-2, Figures 4-1 and 4-2). Location 15.9, the uppermost location, had significantly higher densities than Mixing Zone locations during all sampling periods except February (Table 4-2). In most previous years of the Program, 4-2

Background Locations had higher mean densities than Mixing Zone locations (Duke Power Company 1988, 1989, 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997; Duke Power 1998, 1999, 2000, 2001,2002, 2003,2004).

Historically, both seasonal and spatial variability among epilimnetic zooplankton densities have been much higher among Background Locations than among Mixing Zone locations.

The uppermost location, 15.9, showed the greatest range of densities during 2004 (Table 4-1, Figures 4-3 and 4-4). Apparently epilimnetic zooplankton communities are more greatly influenced by environmental conditions at the up-lake locations than at the down-lake locations. Location 15.9 represents the transition zone between river and reservoir where populations would be expected to fluctuate due to the dynamic nature of this region of Lake Norman. At the locations nearest the dam (Locations 2.0 and 5.0), seasonal variations are dampened and the overall production would be lower due to the relative stability of this area (Thornton, et al. 1990). A similar trend was observed in the phytoplankton communities (Chapter 3).

Epilimnetic zooplankton densities during 2004 were generally within historical ranges during each quarter (Figures 4-3 and 4-4). The exceptions were Location 5.0, which had the highest density yet recorded for February; and Locations 2.0 and 5.0, which had record high densities for May. Phytoplankton chlorophyll concentrations and standing crops during 2004 also fell within historical ranges on most occasions; however, down-lake locations demonstrated historical chlorophyll minima for May, which may indicate an inverse relationship between phytoplankton and zooplankton standing crops over the long term (Chapter 3).

The highest February densities recorded from Locations 2.0 and 11.0 occurred in 1996, while February maxima at Locations 9.5 and 15.9 were recorded for 1995 and 1992, respectively.

The February maximum form Location 5.0 occurred in 2004 (Figure 4-3). Long term maximum densities for May at Locations 2.0 and 5.0 were observed in 2004, while the highest May values from Locations 11.0 and 15.9 occurred in 2002. The long term May peak from Location 9.5 was observed in 2000. Long term August maxima occurred in 1988 at all but Location 15.9, which had its highest August value in 2003 (Figure 4-4). November long term maxima at Locations 2.0 through 9.5 occurred in 1988, and at Locations 11.0 and 15.9 in November 1999.

4-3

Since 1990, the densities at Mixing Zone Locations in May, August, and November have shown a moderate degree of year-to-year variability, and the long term trend at Mixing Zone locations has been a gradual increase over the last fifteen years with long term peaks recorded in 2004. Year-to-year fluctuations in densities during February have occasionally been quite striking, particularly between 1991 and 1997. The Background Locations continue to exhibit considerable year-to-year variability in all seasons (Figures 4-3 and 4-4).

Community Composition One hundred and seventeen zooplankton taxa have been identified since the Lake Norman Maintenance Monitoring Program began in August 1987 (Table 4-3). Fifty-two taxa were identified during 2004, as compared to fifty-one taxa recorded during 2003 (Duke Power 2004). Four previously unreported taxa were identified in 2004: Three cladocerans (Disparalonaacutirostris, Leydigia acanthoceroides, and Oxurella spp.), and one rotifer (Anuraeopsisfissa)were added to the taxa list.

Copepods, which were most often dominant during 2001, showed a significant decline in relative abundance during 2002, when they were dominant in only seven August samples.

During 2003, copepods rebounded considerably, and were dominant in thirteen zooplankton samples collected during all four quarters (Duke Power 2002, 2003, and 2004). During 2004, copepod dominance and relative abundance declined once more, and these microcrustaceans were dominant in only ten samples collected in August and November (Table 4-1, Figures 4-2 and 4-6 through 4-8). Copepods were dominant in epilimnetic samples from Location 5.0, 11.0, and 15.9 in August; and Location 2.0 in November. They dominated whole column samples at all locations in August and Location 2.0 in November.

Cladocerans, always the least abundant forms in Lake Norman, were dominant in only two epilimnetic samples from Locations 2.0 and 9.5 in August. Rotifers were dominant in 70%

of all zooplankton samples collected during 2004. During most years of the Program, microcrustaceans (copepods and cladocerans) dominated Mixing Zone samples, but were considerably less important among Background Locations (Figures 4-6 through 4-8). From 1995 through 1998, a trend of increasing relative abundance among microcrustaceans was observed throughout Lake Norman. Since 2000, this trend has reversed, with a subsequent increase in relative abundances of rotifers to the extent that taxonomic composition since 2002 has been similar to that found during 1995. During 2004, microcrustaceans decreased in relative abundance in all areas of Lake Norman.

4-4

Copepoda Copepod populations were consistently dominated by immature forms (primarily nauplii) during 2004, as has always been the case. Adult copepods rarely constituted more than 10%

of the total zooplankton density at any location. Tropocyclops was the most important constituent of adult populations in both epilimnetic and whole column samples (Table 4-4).

This was also the case in previous years (Duke Power 2004).

Copepods tended to be more abundant, if not dominant, at Background Locations than at Mixing Zone Locations during 2004, and their densities peaked in May at both Mixing Zone and Background Locations. Copepods showed similar spatial and seasonal trends during 2003 (Table 4-1, Figure 4-5). Historically, maximum copepod densities were most often observed in May.

Cladocera Bosmina was the most abundant cladoceran observed in 2004 samples, as has been the case in most previous studies (Duke Power 2004, Hamme 1982). Bosmina often comprised greater than 5% of the total zooplankton densities in both epilimnetic and whole column samples, and was the dominant zooplankter in several samples from November (Table 4-4).

Bosminopsis was also important among cladocerans in August when it dominated cladoceran populations at most locations. Bosminopsis expressed somewhat lower dominance during August 2004 as compared to August 2003. Similar patterns of Bosminopsis dominance have been observed in past years of the Program (Duke Power 2004).

Long-term seasonal trends of cladoceran densities were variable: From 1990 to 1993, peak densities occurred in February; while in 1994, 1995, 1997, 2000, and 2004, maxima were recorded in May (Figure 4-5). During 1996, 1999, and 2002, peak cladoceran densities occurred in May in the Mixing Zone, and in August among Background Locations.

Maximum cladoceran densities in 1998 occurred in August. In 2001, maximum cladoceran densities in the Mixing Zone occurred in February, while Background Locations showed peaks in November. During 2003, maximum densities at Background Locations occurred in August, while peaks in the Mixing Zone were observed in November. Spatially, cladocerans

_s were well distributed among all locations (Table 4-1, Figures 4-2 and 4-5).

4-5

Rotifera Polyarthra was the most abundant rotifer in 2004 samples (Table 4-4). This taxon dominated rotifer populations in the epilimnion at Locations 2.0 and 9.5; and Location 15.9 (whole column) in February; was dominant at all locations in May, and in whole column samples from Locations 11.0 and 15.9 in August. In November, Polyarthra was the dominant rotifer at all but Locations 5.0 (epilimnion) and 15.9. Conochilus dominated rotifer populations at Locations 2.0, and 9.5; and were dominant in whole column samples at Locations 11.0 and 15.9 in August. Keratella was the dominant rotifer at Location 11.0 (epilimnion) in February, Location 5.0 (epilimnion) in August, and Locations 5.0 (epilimnion) and 15.9 in November. Asplanchna was the dominant rotifer at Location 15.9 (epilimnion), as well as in whole column samples from Locations 9.5 and 11.0 in February.

Synchaeta was the dominant rotifer at Location 5.0 in February. All of these taxa have been identified as important constituents of rotifer populations, as well as zooplankton communities, in previous studies (Duke Power 2004; Hamme 1982).

Long term tracking of rotifer populations indicated high year-to-year seasonal variability.

Peak densities have most often occurred in February and May, with an occasional peak in August (Figure 4-5, Duke Power 1989, 2002, 2003, 2004). During 2004, peak densities were observed in May.

FUTURE STUDIES No changes are planned for the zooplankton portion of the Lake Norman Maintenance Monitoring Program in 2005.

SUMMARY

Maximum zooplankton densities occurred in May at Locations 2.0, 5.0, and 15.9, while maximum densities at Locations 9.5 and 11.0 were observed in February. Minimum zooplankton densities were most often noted in August. As in past years, epilimnetic densities were higher than whole column densities. Mean zooplankton densities tended to be higher among Background Locations than among Mixing Zone Locations during 2004. In the Mixing Zone, a long term trend of increasing year-to-year densities was observed for 4-6

May. In addition, long term trends showed much higher year-to-year variability at Background Locations than at Mixing Zone Locations.

Epilimnetic zooplankton densities were generally within ranges of those observed in previous years. The exceptions were record high densities for February at Location 5.0, and record high May densities at Locations 2.0, 5.0, and 15.9. Long term May maxima at Locations 2.0 and 5.0 corresponded to record low May phytoplankton chlorophyll concentrations at these locations.

One hundred and seventeen zooplankton taxa have been recorded from Lake Norman since the Program began in 1987 (Fifty-two were identified during 2004). Four previously unreported taxa (three cladocerans and one rotifer) were identified during 2004.

Overall relative abundance of copepods in 2004 had decreased since 2003, and they were dominant in ten samples collected during August and November. Cladocerans were dominant in only two samples in August, while rotifers were dominant in 70% of all samples.

Overall, the relative abundance of rotifers had increased since 2003, and their relative abundances were somewhat similar to those of 1995. Historically, copepods and rotifers have most often shown annual peaks in May; while cladocerans continued to demonstrate year-to-year variability.

Copepods were dominated by immature forms with adults rarely accounting for more than 10% of zooplankton densities. The most important adult copepod was Tropocyclops, as was the case in previous years. Bosmina was the predominant cladoceran, as has also been the case in most previous years of the Program. Bosminopsis dominated most cladoceran populations in August. The most abundant rotifers observed in 2004, as in many previous years, were Polyarthra, Conochilus, and Karetella, while Asplanchna and Syncheata were occasionally important among rotifer populations.

Lake Norman continues to support a highly diverse and viable zooplankton community.

Zooplankton densities, as well as seasonal and spatial trends were generally consistent with historical precedent during 2004, and no impacts of plant operations were observed.

4-7

LITERATURE CITED Duke Power Company. 1976. McGuire Nuclear Station, Units 1 and 2, Environmental Report, Operating License Stage. 6th rev. Volume 2. Duke Power Company, Charlotte, NC.

Duke Power Company. 1985. McGuire Nuclear Station, 316(a) Demonstration. Duke Power Company, Charlotte, NC.

Duke Power Company. 1988. Lake Norman Maintenance Monitoring Program: 1987 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1989. Lake Norman Maintenance Monitoring Program: 1988 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1990. Lake Norman Maintenance Monitoring Program: 1989 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1991. Lake Norman Maintenance Monitoring Program: 1990 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1992. Lake Norman Maintenance Monitoring Program: 1991 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1993. Lake Norman Maintenance Monitoring Program: 1992 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1994. Lake Norman Maintenance Monitoring Program: 1993 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1995. Lake Norman Maintenance Monitoring Program: 1994 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1996. Lake Norman Maintenance Monitoring Program: 1995 summary. Duke Power Company, Charlotte, NC.

Duke Power Company. 1997. Lake Norman Maintenance Monitoring Program: 1996 summary. Duke Power Company, Charlotte, NC.

Duke Power. 1998. Lake Norman Maintenance Monitoring Program: 1997 Summary. Duke Energy Corporation, Charlotte, NC.

Duke Power. 1999. Lake Norman Maintenance Monitoring Program: 1998 Summary. Duke Energy Corporation, Charlotte, NC.

4-8

Duke Power. 2000. Lake Norman Maintenance Monitoring Program: 1999 Summary. Duke Energy Corporation, Charlotte, NC.

Duke Power. 2001. Lake Norman Maintenance Monitoring Program: 2000 Summary. Duke Energy Corporation, Charlotte, NC.

Duke Power. 2002. Lake Norman Maintenance Monitoring Program: 2001 Summary. Duke Energy Corporation, Charlotte, NC.

Duke Power. 2003. Lake Norman Maintenance Monitoring Program: 2002 Summary. Duke Energy Corporation, Charlotte, NC.

Duke Power. 2004. Lake Norman Maintenance Monitoring Program: 2003 Summary. Duke Energy Corporation, Charlotte, NC.

Hamme, R. E. 1982. Zooplankton, In J. E. Hogan and W. D. Adair (eds.). Lake Norman Summary, Technical Report DUKEPWR/82-02. p. 323-353, Duke Power Company, Charlotte, NC. 460 p.

Hutchinson, G. E. 1967. A Treatise on Limnology. Vol. II. Introduction to Lake Biology and the Limnoplankton. John Wiley and Sons, Inc. N. Y. 1115 pp.

Menhinick, E. F. and L. D. Jensen. 1974. Plankton populations. In L. D. Jensen (ed.).

Environmental responses to thermal discharges from Marshall Steam Station, Lake Norman, North Carolina. Electric Power Research Institute, Cooling Water Discharge Research Project (RP-49) Report No. 11., p. 120-138, Johns Hopkins University, Baltimore, MD 235 p.

Thornton, K. W., B. L. Kimmel, F. E. Payne. 1990. Reservoir Limnology. John Wiley and Sons, Inc. New York, NY.

4-9

Table 4-1. Total zooplankton densities (no. X 1000/m 3 ), densities of major zooplankton taxonomic groups, and percent composition (in parentheses) of major taxa in lOm to surface (l0-S) and bottom to surface (B-S) net tow samples collected from Lake Norman in February, May, August, and November 2004.

Sample Locations Date Type Taxon 2.0 5.0 9.5 11.0 15.9 2/11/04 10-S COPEPODA 9.2 6.5 31.3 13.6 19.2 (16.8) (6.6) (25.7) (8.0) (22.0)

CLADOCERA 8.6 5.6 21.1 15.1 11.1 (15.6) (5.7) (17.3) (8.9) (12.8)

ROTIFERA 37.2 85.9 69.4 140.9 56.9 (67.6) (87.7) (57.0) (83.1) (65.2)

TOTAL 55.0 98.0 121.8 169.6 87.2 B-S Depth (m) of tow COPEPODA 7.3 5.8 24.8 13.6 19.7 For each (14.7) (10.6) (29.5) (10.5) (24.7)

Location CLADOCERA 10.7 3.3 15.2 17.2 8.0 2.0=30 (21.5) (6.1) (18.1) (13.2) (10.1) 5.0=20 ROTI FERA 31.8 45.7 44.2 99.2 51.8 9.5=20 (63.8) (83.3) (52.5) (76.3) (65.2) 11.0=25 15.9=20 TOTAL 49.8 54.8 84.3 130.0 79.5 5/13/04 10-S COPEPODA 50.8 53.9 34.4 30.9 60.7 (28.3) (30.4) (31.9) (25.3) (16.9)

CLADOCERA 10.8 10.8 4.5 7.6 45.7 (6.0) (6.1) (4.2) (6.3) (12.7)

. ROTIFERA 117.9 112.8 69.0 83.3 253.0 (65.7) (63.5) (63.9) (68.4) (70.4)

TOTAL 179.6 177.5 108.0 121.8 359.4 B-S Depth (m)

Of tow COPEPODA 24.8 32.8 23.2 20.6 35.4 for each (26.9) (29.6) (28.5) (30.0) (19.0)

Location CLADOCERA 17.3 8.9 4.9 3.6 17.1 2.0=30 (18.8) (8.0) (6.0) (5.2) (9.2) 5.0=20 ROTIFERA 50.3 69.1 53.2 44.6 133.8 9.5=20 (54.3) (62.4) (65.5) (64.8) (71.8) 11.0=25 15.9=20 TOTAL 92.4 110.8 81.3 68.8 186.3 4-10

Table 4-1. (Continued).

Sample Locations Date Type Taxon 2.0 5.0 9.5 11.0 15.9 8/4/04 10-S COPEPODA 13.4 18.2 9.0 38.7 41.9 (45.5) (56.2) (28.3) (62.7) (43.6)

CLADOCERA 13.4 8.1 17.2 7.2 20.0 (45.6) (24.9) (54.2) (11.8) (20.8)

ROTIFERA 2.6 6.1 5.5 15.7 34.2 (8.9) (18.8) (17.5) (25.4) (35.6)

TOTAL 29.4 32.4 31.7 61.7* 96.1 B-S Depth(m) of tow COPEPODA 12.3 15.4 11.8 24.6 29.0 for each (63.5) (65.4) (43.5) (71.6) (47.6)

Location CLADOCERA 5.9 4.8 11.5 4.9 10.8 2.0=30 (30.2) (20.6) (42.2) (14.4) (17.7) 5.0=19 ROTIFERA 1.2 3.3 3.8 4.8 21.0 9.5=20 (6.3) (14.0) (14.1) (14.0) (34.4) 11.0=25 15.9=21 TOTAL 19.4 23.5 27.1 ** 34.3 61.0**

11/30/04 10-S COPEPODA 13.0 9.1 13.2 26.0 24.8 (40.5) (26.3) (26.0) (27.8) (19.6)

CLADOCERA 6.9 11.9 17.4 11.0 4.2 (21.5) (34.1) (34.2) (11.7) (3.3)

ROTIFERA 12.1 13.8 20.2 56.5 97.5 (38.0) (39.6) (39.8) (60.4) (77.1)

TOTAL 32.0 34.8 50.9 93.5 126.5 B-S Depth(m) of tow COPEPODA 11.0 7.5 14.6 26.5 16.3 For each (44.8) (28.5) (32.1) (34.2) (19.9)

Location CLADOCERA 7.0 8.4 9.0 10.3 4.1 2.0=30 (28.7) (32.1) (19.8) (13.3) (5.0) 5.0=20 ROTIFERA 6.5 10.3 21.9 40.7 61.5 9.5=21 (26.5) (39.4) (48.1) (52.5) (75.1) 11.0=25 15.9=20 TOTAL 24.5 26.2 45.6 77.6 81.9

  • = Ostracoda observed in sample (103/m 3 , 0.17%).
    • = Chaoborus(Insecta) observed in sample at 9.5 (53/m 3 , 0.19%), and 15.9 (207/m 3 ,

0.34%

4-11

Table 4-2. Duncan's Multiple Range Test on epilimnetic zooplankton densities (no. X 1000/m 3 ) in Lake Norman, NC during 2004.

February Location 2.0 15.9 5.0 9.5 11.0 Means 55.0 87.2 98.0 121.8 169.6 May Location 9.5 11.0 5.0 2.0 15.9 Means 108.0 121.8 177.5 179.6 359.4 August Location 2.0 9.5 5.0 11.0 15.9 Means 29.4 31.7 32.4 61.7 96.1 November Location 2.0 5.0 9.5 11.0 15.9 Means 32.0 34.8 50.9 93.5 126.5 4-12

Table 4-3. Zooplankton taxa identified from samples collected quarterly on Lake Norman from 1987 through 2004.

TAXON 87-90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 COPEPODA Cyclops thomasi Forbes X = - - X X X X X X X X X X C. vernalisFischer X _

C. spp. O. F. Muller X X X X X X X X X X X X Diaptomus birgei Marsh X X X_ X _X D. mississippiensis Marsh X X X X X X X X X X X X X X X D. pallidus Herick X __= X - X X X - X X =

D. reighardiMarsh X D. spp. Marsh X X X X X X X X X X X Xx X EpishurafluviatilisHerrick X X X X X X X X X X Ergasilus spp. X Eucyclops agilis (Koch) X Mesocyclops edax (S. A. Forbes) X - = - X X X X X X X X X X X M. spp. Sars X X X X X X X X X X X Tropocyclops prasinus (Fischer) X X X X X X X X X X X T. spp. (Fischer) X X X X X X X X XX X Calanoid copepodites X X X X X X X X X X X X X X X Cyclopoid copepodites X X X X X X X X X X X X X X X Harpacticoidea X X X Nauplii X X X X X X XXX X X X X XX Parasitic copepods X CLADOCERA Alona spp.Baird X X Alonella spp. (Birge) X X _

Bosmina longirostris(O. F. M.) X X X X X X X X X X B. spp. Baird X X X X X X X X X X X X Bosminopsis dietersi Richard X X X X X X X X X X X X X X X Ceriodaphnialacustris Birge X X X X X X X X C. spp. Dana X X X X X XX X X X X X X X Chydorusspp. Leach X X X X X X X X X X Daphnia ambigua Scourfield X __ X X X X X D. catawba Coker X X X D. galeata Sars X D. laevis Birge X X D. Iongiremis Sars X X X X X X D. lumholzi Sars X X X X X X X X D. mendotae (Sars) Birge x X X X X D. parvula Fordyce X X X X X X x X x x x D. pulex (de Geer) X X D. pulicariaSars X X D. retrocurva Forbes X X X X X X X X D. schodleri Sars X D. spp. Mullen X X XX X X X X XX X X X X X Diaphanosomabrachvurumn (Lievin) X X X X X X X X 4-13

n _r

_1 .. A - A_ .____ 1 I able 4-i (continuea) page__ o i TAXON 87-90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 D. spp. Fischer X X XX X X X X X X X X X X Disparalonaacutirostris(Birge) x Eubosmina spp. (Baird) _ X _

Holopedium amazonicum Stin.. X - X X X X X X X H. gibberum Zaddach X _ __ X X H. spp. Stingelin X X X X X X X X X X X X -

Ilyocryptus sordidus (Lieven) X

1. spinifer Herrick X

. spp. Sars X X X =__ X X Latona setifera (O.F. Muller) X Leptodora kindtii (Focke) X X X X X X X X X X X X X X Leydigia acanthoceroides (Fis.) ==== == X L. spp. Freyberg X X X X X _X X Moina spp. Baird X __

Monospilus dispar Sars x X

Oxurella spp. (Sars) X Pleuroxus hamulatus Birge X P. spp. Baird X Sida crystallina 0. F. Muller X X X Simocephalus expinosus (Koch) X Simocephalus spp. Schodler X ROTIFERA __

Anuraeopsisfissa(Gosse) = = - = = = -= = = X A. spp. Lauterborne X X X X X X X x Asplanchna brightwelli Gosse X X A. priodonta Gosse X X X X A. spp. Gosse X X X x X X X X x x x X X x x Brachionus caudataBar. & Dad. X __ _

B. bidentata Anderson J X X

B. havanensis Rousselet X X B. patulus 0. F. Muller X X B. spp. Pallas X X X X X Chromogasterovalis (Berg.) X X X X C. spp. Lauterbome X X I X X 7X X Collotheca balatonicaHarring 1 X X X X X X X X C. mutabilis (Hudson) X X X X X X X C. spp. Harring X X X x X X XX X X x X X Colurella spp. Bory de St. Vin. X ConochiloidesdossuariusHud. x x x x X X X C. spp. Hlava X xX x x X7 X - X Conochilus unicornis(Rouss.) X I__ X X X X X X X X C. spp. Hlava X X X X X XX X X X Filiniaspp. Bory de St. Vincent X X X X Gastropus stylifer Imhof X X X X X G. spp. Imhof X X X X X X X X X Hexarthramira Hudson X X X X X H. spp. Schmada X X X X X X X X X Kellicottia bostoniensis (Rouss.) X X XX X X X X X X X K longispina Kellicott _X X X X X X X X 4-14

Table 4-3 (continued) )age 3 of 3 TAXON 87-90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 K spp. Rousselet X X X X X X X X XX X X Keratellacochlearis X X X K taurocephalaMyers _ --- X X X K spp. Bory de St. Vincent X X XX X X X X X X X X X X Lecane spp. Nitzsch X X X X X X X X X MacrochaetussubquadratusP. X X M spp. Perty X X X X X X = X Monostyla stenroosi (Meiss.) X ___

M spp. Ehrenberg X XX XX X Notholca spp. Gosse = = XX _ X X Platyiaspatulus Harring X Ploeosoma hudsonii Brauer X X X X X X X X X _X X X P. truncatum (Levander) X X X X X X X X X X X X P. spp. Herrick X X X X X X X X X Polyarthraeuryptera (Weir.) X _ __ X P. major Burckhart X X X = X X X P. vulgaris Carlin X X X X X X X X P. spp. Ehrenberg X X X X XX X X X X X X X X X Pompholyx spp. Gosse X ====

Ptyguralibra Meyers X X X X X X P. spp. Ehrenberg X X X X X X X X X Synchaeta spp. Ehrenberg X X X X X X X X X X X X X X X Trichocercacapucina (Weir.) X X X X X X X T.cylindrica (Imhof) X X X X X X X X X X T. longiseta Schrank X T. multicrinis (Kellicott) X X X X X X T. porcellus (Gosse) X X X X X X X T. pusilla Jennings X T.similis Lamark X T.spp. Lamark X X X X X X X X X X XX X XX Trichotria spp. Bory de St. Vin. X X X Unidentified Bdelloida X X X X X X X X Unidentified Philodinidae X Unidentified Rotifera X X X X X X X X X X INSECTA Chaoborusspp. Lichtenstein X X X X X X X X X OSTRACODA (unidentified) X X 4-15

Table 4-4. Dominant taxa among copepods (adults), cladocerans, and rotifers, and their percent composition (in parentheses) of copepod, cladoceran and rotifer densities in Lake Norman samples during 2004.

FEBRUARY MAY AUGUST l NOVEMBER COPEPODA EPILIMNION 2.0 Tropocyclops (6.8)* Tropocyclops (4.6) Tropocyclops (4.8) Tropocyclops (7.8)*

5.0 No adults Mesocyclops (7.3.) Tropocyclops (4.6)* Tropocyclops (2.1) 9.5 Cyclops (3.0) Epishura (7.5) Tropocyclops (5.3)* Tropocyclops (3.0) 11.0 Tropocyclops (5.3)* Tropocyclops (3.5) Tropocyclops (10.4) Meso/Tropo (0.8 ea.)*

15.9 Tropocyclops (4.5.) Tropocyclops (3.8) Tropocyclops (13.4) Tropocyclops (1.3)*

COPEPODA WHOLE COLUMN 2.0 Tropocyclops (7.4) Tropocyclops (3.3) Mesocyclops (11.6) Tropocyclops (6.7) 5.0 Tropocyclops (19.3) Epishura (9.0) Tropocyclops (2.8) Tropocyclops (7.3) 9.5 Tropocyclops (8.3) Tropocyclops (4.8) Tropocyclops (8.6) Tropocyclops (3.6) 11.0 Tropocyclops (4.7)* Mesocyclops (5.6) Tropocyclops (7.7) Mesocyclops (1.9) 15.9 Cyclops (2.5) Mesocyclops (4.4) Mesocyclops (4.6) Tropocyclops (3.3)

CLADOCERA EPILIMNION 2.0 Bosmina (100.0) Bosmina (85.8) Bosminopsis (93.8) Bosmina (96.9) 5.0 Bosmina (100.0) Bosmina (86.3) Bosminopsis (90.7) Bosmina (93.6) 9.5 Bosmina (100.0) Bosmina (68.7) Bosminopsis (96.5) Bosmina (98.8) 11.0 Bosmina (97.5) Bosmina (47.6) Bosminopsis (73.0) Bosmina (99. 1) 15.9 Bosmina (97.6) Daphnia(60.0) Bosmina (62.1) Bosmina (100.0)

CLADOCERA WHOLE COLUMN 2.0 Bosmina (98.7) Bosmina (94.1) Bosminopsis (77.2) Bosmina (86.7) 5.0 Bosmina (100.0) Bosmina (83.0) Bosminopsis (81.1) Bosmina (89.7) 9.5 Bosmina (98.8) Bosmina (84.4) Bosminopsis (85.6) Bosmina (9 1.1) 11.0 Bosmina (97.7) Bosmina (60.0) Bosmina (43.5) Bosmina (88.9) 15.9 Bosmina (98.1) Daphnia(46.4) Bosmina (56.7) Bosmina (100.0) 4-16

Table 4-4. (Continued)

FEBRUARY MAY AUGUST NOVEMBER ROTIFERA EPILIMNION 2.0 Polyarthra(44.1) Polyarthra(92.4) Conochilus (37.7) Polyarthra(55.4) 5.0 Synchaeta (66.5) Polyarthra(96.6) Keratella(26.6) Keratella (41.2) 9.5 Polyarthra(47.3) Polyarthra(93.7) Conochilus (41.4) Polyarthra(67.6) 11.0 Keratella (45.4) Polyarthra(90.1) Conochilus (35.8) Polyarthra(53.0) 15.9 Asplanchna (32.7) Polyarthra(43.0) Conochilus (38.0) Keratella (65.6)

ROTIFERA WHOLE COLUMN 2.0 Asplanchna (47.0) Polyarthra(82.3) Conochilus (51.4) Polyarthra(52.9) 5.0 Synchaeta (83.9) Polyarthra(75.2) Trichocerca(46.4) Polyarthra(58.2) 9.5 Asplanchna (68.9) Polyarthra(70.1) Conochilus (52.0) Polyarthra(77.2) 11.0 Asplanchna (63.5) Polyarthra(52.9) Polyarthra(47.3) Polyarthra(62.9) 15.9 Polyarthra(47.9) Polyarthra(75.8) Polyarthra(35.2) Keratella(46.6)

  • = Only adults present in samples.

4-17

10m TO SURFACE TOWS 4UU C'0 z

10- ---- -- - -=-- --

0 2.0 5.0 9.5 11.0 15.9 BOTTOM TO SURFACE TOWS

-+-FEB MAY AUG -*-NOV Ann 350 ---------------------------------------------------------

300 --------------------------------------------------------

-E 250 -------------------------------------------------------

C~

! 200 -------------------------------------------------------

x I z 150 ------------------------------------------

100 ----------


-------- --- I 50 0

0 i 2.0 5.0 9.5 11.0 '15.9 LOCATIONS Figure 4-1. Total zooplankton density by location for samples collected in Lake Norman, NC, in 2004.

4-18

400 400 360- 360 320 320

";280- 280 2240 240 6200 200 I

,,g160 160 z

120

," 20 80~S 80 40 U U 2.0 5.0 9.5 11.0 15.9 2.0 5.0 9.5 11.0 15.9 AUGUST _

180 160 140

D20
1oo
00 C
_ 80 x 60 Co Lu 0

40 20 0

2.0 5.0 9.5

! ,i 11.0 15.9 2.0 5.0 9.5 11.0 15.9 LOCATIONS mOCOPEPODS i _ CLADOCERANS ROTIFERS Figure 4-2. Zooplankton community composition by month for epilimnetic samples collected in Lake Norman, NC, in 2004.

4-19

MIXING ZONE FEBRUARY 250 _ Vmy 250 225 -- -2.O 5.0 - - - - - - - - - - - - - 225 - - - - - - - - - - - - - - - - - - - - - - - - - - _

200 2 00 - - - - - - - - - - - - - - - - - - - - - - - - - - _

r 175 175 - - - - - -- - - -- - - -- - - -- - - -- - - -

° 150 150 - - - - - - - - - - - - - - - - - - - - - - -a x

6 125 Cn F 100 150-00 - - - - - - - - - -- - - -

z 75 175 50 AAAA- 2.

25 50 I

87 88 89 90 91 92 93 94 95 96 97 98 99 00 0102 03 04 87 88 89 90 9192 93 94 95 96 97 98 99 00 0102 03 04 BACKGOUND LOCATIONS

+

87 88 89 90 9192 93 94 95 96 97 98 99 00 0102 03 04 87 88 89 90 9192 93 94 95 96 97 98 99 00 01 02 03 04 YEARS YEARS Figure 4-3. Total zooplankton densities by location for epilimnetic samples collected in Lake Norman, NC, in February and May of 1988 through 2004.

4-20

MIXING ZONE AUGUST 250 225 -- 4+2.0 --. 01 -

200 r 175 o 150 x

o 125 ______------------------1 I- 100 z A------------- -- _----

LU a 75 50 25' 87 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 87 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 BACKGROUND LOCATIONS 87 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 87 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 YEARS YEARS Figure 4-4. Total zooplankton densities by location for epilimnetic samples collected in Lake Norman, NC, in August and November of 1987 through 2004.

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1 AfnlfWl AKIC 60 -

-4MIXING ZONE -U- BACKGROUND LOCATIONS g 40 - -_ - --- . __- ___- ___-.__

o 30 - -- - -------

z

'U 10 150- ___--_ _--__- __-- _-- __-___-___-___ ---- - ---- ---------------

300 250IFR 100 a) a a a a 9Da9 z

aa c

u P S? @

a 9

a D9 a; a o9 a a

@9 a 8 89 E 9

E 9

8 o 8 o a Z Figure 4-5. Zooplankton composition by quarter for epimlimnetic samples collected in Lake Norman, NC, from 1990 through 2004.

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LAKE-WIDE: EPILIMNION lOCOPEPODS OCLADOCERANS *ROTIFERS z

0 r-z C

IL a.-

0o ) 0 _ N 0 tt U0 O P- W 0O - N 0 t c 0 ta) a O) a)0)0) a)0, 0 0 0 a 0 0 a) 0 a ) a) 0 0 D a) 0 0 0o 0 0

_ _ _ _ _ _ C_4N N N N N YEARS LAKE-WIDE: WHOLE COLUMN COPEPODS MCLADOCERANS EROTIFERSI 100%

90%

z 80%

° L 70%

cn go 60%

o 50%

IL) z 40%

LU

(* 30%

CL 20%

10%

0%

0G 0> 0 - N M IV W CD 0) 0) 0 - N M 0 CD 0) 0) 0) 0) a) 0) 0) 0) 0) 0) 0 0 0 0 0 a> a) a) oa a: a) a) a a 0) a) 0 00 0 0 r _N N N N N YEARS Figure 4-6. Annual lake-wide percent composition of major zooplankton taxonomic groups from 1988 through 2004 (Note: Does not include Location 5.0 in November 2002).

4-23

MIXING ZONE (LOCATIONS 2.0 4 5.0): WHOLE COLUMN ODCOPEPODS 13CLADOCERANS UROTIFERS 100% - - - - -

90%

Z 80%

0 P 70%

o0 60%

0 50%

0 I'

z 40%

030% -

ILl IL 20%- - -- - -- - - - -

10%

co co 0 M CDI U CD M C) M C 0 CD)

CD 0) 0 ) 0 ) 0) m 0 ) ) 0 TT T 0 0 No N, cN NO YEAR Figure 4-7. Annual percent composition of major zooplankton taxonomic groups from Mixing Zone Locations: 1988 through 2004 (Note: Does not include Location 5.0 in November 2002).

4-24

BACKGROUND (LOCATIONS 9.5 + 11.0 + 15.9): EPILIMNION OECOPEPODS U3CLADOCERANS U ROTIFERSI 100%

90%

0 70%-

0.

o 50% -

I-' 40%-

z o 30%

LUJ 20%

a-I100%

0%

Co <D CO CO CO co CD CO co C CD CO 0 a 0 0 0 co CO CO

<D C C CO CO C w0 CO C O O O 0D co 0 - ) t D. CA 0) -4 co co 0 N)

" C.)

YEAR Figure 4-8. Annual percent composition of major zooplankton taxonomic groups from Background Locations: 1988 through 2004.

4-25

CHAPTER 5 FISHERIES INTRODUCTION In accordance with the NPDES permit for McGuire Nuclear Station (MNS), monitoring of specific fish population parameters continued during 2004. The components of this portion of the Lake Norman Maintenance Monitoring Program were to continue:

1. spring electrofishing surveys of littoral fish populations with emphasis on age, growth, size distribution, and relative weight (Wr) of largemouth bass (scientific names of fish mentioned in this chapter are listed in Table 5-1);
2. summer striped bass mortality monitoring;
3. cooperative striped bass study with the North Carolina Wildlife Resources Commission (NCWRC) with emphasis on age, growth and Wr;
4. cooperative trap-net surveys with NCWRC for white crappies and black crappies, with emphasis on age and growth;
5. small mesh gill-net surveys to determine vertical distributions of prey fish in summer;
6. fall hydroacoustic and purse seine surveys of pelagic prey fish to determine their abundance and species composition.

METHODS AND MATERIALS Spring electrofishing surveys were conducted in March and April at three locations: (1) near the Marshall Steam Station (MSS) in Zone 4, (2) a reference (REF) area located between MNS and MSS in Zone 3 and (3) near the MNS in Zone 1 (Figure 5-1). The locations sampled in 2004 were the same sampled since implementation of this sampling program in 1993 and consisted of ten 300-in shoreline transects in each location. All transects included the various types of fish habitat found in Lake Norman. The only areas excluded were shallow flats where the boat could not access the area within 3-4 m of the shoreline. All sampling was conducted during daylight, when water temperatures generally ranged from 15 to 20 'C (59 to 68 'F). All stunned fish were collected and identified to species. Except for largemouth bass and spotted bass, all other fish were counted and weighed (g) in aggregate by taxon. Individual total lengths (mm) and weights were obtained for all largemouth bass 5-1

and spotted bass collected. Sagittal otoliths were removed from all largemouth bass and sectioned for age determination (Devries and Frie 1996). Growth rates were calculated as the mean length for all fish of the same age. Relative weight was calculated for all largemouth bass > 150 mm long, using the formula Wr = (W/Ws) x 100, where W = weight of the individual fish (g) and W. = length-specific mean weight (g) for a fish as predicted by a weight-length equation for that fish (Anderson and Neumann 1996).

Mortality surveys for striped bass were conducted from July 1 through August 30. Initially, roving surveys were conducted weekly (as in previous years) to specifically search for dead or dying striped bass in Zones 1-4. After considerable striped bass mortality was noted on July 22 and again on July 27, surveys were conducted every other day until July 30. After this date, daily surveys were implemented and continued through August 13. After August 13, weekly surveys were again conducted through August 30. All dead and dying striped bass were collected during these surveys and their location noted. Measurements for total length were also obtained for a portion of these fish prior to their disposal.

Striped bass for age, growth and Wr calculations were collected at a local fishing tournament in late November and gill-net surveys conducted in early December by NCWRC and Duke Power (DP) personnel. Individual total lengths and weights were obtained, and sagittal otoliths were removed from each striped bass. Age, growth and Wr were determined for these fish as described earlier for largemouth bass.

White crappie and black crappie populations in Lake Norman were sampled cooperatively by the NCWRC and DP in late October and early November using trap nets as described by Nelson and Dorsey (2005). Total lengths and weights were obtained for all collected white and black crappies and sagittal otoliths were removed for age and growth determinations.

Fish inhabiting Lake Norman's hypolimnion in Zone 1 during July were sampled using small-mesh gill nets that were 45.7 m long x 2.7 m deep containing one 7.6-m panel of 10-,

13-, 19-, 25-, 32- and 38-mm mesh (square measure) to determine species composition. Four nets were fished in the upper portion of the hypolimnion at a depth of 18.3 m where daytime mobile hydroacoustic samples indicated fish were most abundant (unpublished Duke Power data) and two additional nets were fished on the bottom at a depth of 32.0 m. All nets were set overnight for two consecutive nights, but were retrieved daily, the fish removed and the nets reset. All fish caught were identified to species and enumerated.

5-2

The abundance and distribution of pelagic prey fish in Lake Norman was determined using mobile hydroacoustic (Brandt 1996) and purse seine (Hayes et al. 1996) techniques. The mobile hydroacoustic survey of the entire lake was conducted on September 20 and 22 to estimate forage fish populations. Hydroacoustic surveys employed multiplexing, side-scan and down-looking transducers to detect surface-oriented fish and deeper fish (from 2.0 m below the water surface to the bottom), respectively. Both transducers were capable of determining target strength directly by measuring fish position relative to the acoustic axis.

The lake was divided into six zones (Figure 5-1) due to its large size, spatial heterogeneity, and multiple power generation facilities.

Purse seine samples were collected on September 21 from the lower (Zone 1), mid (Zone 2),

and uplake (Zone 5) areas of the reservoir. The purse seine measured 118 x 9 m with a mesh size of 4.8-mm. A subsample of forage fish collected from each area was used to determine taxa composition and size distribution.

RESULTS AND DISCUSSION Electrofishing resulted in the collection of 2,449 fish (21 species and 2 hybrid complexes) weighing 143 kg from the MSS area, 2,229 fish (17 species and 2 hybrid complexes) weighing 114 kg from the REF area and 1,772 fish (17 species and 2 hybrid complexes) weighing 74 kg from the MNS area (Table 5-2). A variety of species including whitefin shiners, spottail shiners, redbreast sunfish, warmouth, bluegills, redear sunfish, hybrid sunfish and largemouth bass dominated all samples numerically while common carp, redbreast sunfish, bluegills and largemouth bass dominated all samples gravimetrically.

Spotted bass were most abundant (numerically and gravimetrically) in the MNS area, intermediate in abundance in the MSS area and least abundant in the REF area.

Overall, total numbers of fish collected in spring 2004 were highest in the MSS and REF areas, and lowest in the MNS area. Fish biomass at this time was highest in the MSS area, intermediate in the REF area and lowest in the MNS area. Since 1993, the numbers of fish collected in the sampled areas have varied annually (Figure 5-2) with no apparent trend in area catch rates. However, estimates of fish biomass collected annually from 1993 through 2004 have remained generally stable within sampling areas. An exception was noted in 2003 when large numbers of common carp were collected in the MSS area that greatly inflated total fish biomass here over what has been normally observed. Fish biomass noted in 5-3

electrofishing samples was generally highest in the MSS area, intermediate in the REF area, and lowest in the MNS area. This trend in fish biomass continued to support the spatial heterogeneity theory noted by Siler et al. (1986) for fish biomass in Lake Norman. They reported that fish biomass was higher uptake than downlake due to higher levels of nutrients and productivity in the uptake area compared to the downiake area.

The dynamics of the largemouth bass population in Lake Norman was investigated in detail during 2004. Starting in 2000, a noticeable decline in the numbers of largemouth bass in electrofishing samples was observed in all areas of Lake Norman (Figure 5-3). The greatest decline in largemouth bass numbers appeared to occur in the MNS area during 2003 and 2004. However, largemouth bass biomass during this same period did not appear to decline simultaneously with declines in largemouth bass numbers in all areas and even increased in some areas in 2003 and 2004 (Figure 5-3).

In 2004, mean lengths of largemouth bass from Lake Norman varied by area (Table 5-3).

Mean lengths of age 1 largemouth bass were similar in the MSS and MNS areas and fish in these areas were longer than fish from the REF area. Mean lengths of age 2 largemouth bass were highest in the MSS area, intermediate in the REF area and lowest in the MNS area. By age 3, mean lengths of largemouth bass were highest in the REF area, intermediate in the MSS area and lowest in the MNS area. Mean lengths of age 4 largemouth bass was highest in the REF area and lowest, but similar, in the MSS and MNS areas.

Declines noted in largemouth bass numbers that were associated with stable or increasing biomass estimates may indicate improved growth rates for fish in 2003 and 2004 (Table 5-4).

Suspected increases in largemouth bass growth rates in recent years were not readily apparent for age 1 fish, except in those collected from the MSS area for 2003-04 and in the MNS area for 2003 only. There did appear to be some increase in the mean lengths of age 2 and age 3 fish in 2003-04 for most areas when compared to data collected previously (Table 5-4). Mean lengths of age 4 fish in 2003-04 were generally higher in all areas than reported in 1993-94, but not higher than mean lengths reported for the MSS and MNS areas in 1974-78.

In 2004, size distributions of largemouth bass differed somewhat by area (Figure 5-4). The size distributions of largemouth bass collected from the MSS and REF areas were similar with peak numbers occurring at lengths < 150 mm and between 350-399 mm. Even though largemouth bass lengths were somewhat similarly distributed, the numbers of largemouth 5-4

bass 350-399 mm long collected in the MSS area exceeded that noted in the REF area. In contrast to that noted in the MSS and REF areas, only a single peak (200-249) was noted in the size distribution of largemouth bass from the MNS area and the numbers of fish collect here were much lower than noted at the two uplake areas.

The size distributions of largemouth bass in 2004 were generally similar in the MSS and the REF areas to those reported in 2003 (Duke Power 2004), but differed from those observed in the MNS area. In 2003, two peaks (one at 200-249 mm and another at 350-399 mm) were noted in the size distribution of largemouth bass collected from the MNS area. In 2004, only one was observed for fish 200-249 mm long.

Mean Wr for largemouth bass collected from Lake Norman varied somewhat among the three sampling areas (Figure 5-5). Overall, mean Wr calculated for fish from the MSS and REF areas were generally similar for most length groups of fish and were higher than that noted for fish collected from the MNS area. This was especially true for the 150-199 mm length group.

Regarding abundance, growth, size distribution and mean Wr for largemouth bass collected from Lake Norman, it appeared that the dynamics of this fish population continued to change in all sampled areas. Compared to 2003, largemouth bass abundance in 2004 was higher in the MSS and REF areas while remaining similar in the MNS area. However, growth rates declined somewhat for age 1 and age 2 fish in all areas from 2003 to 2004. The size distributions of largemouth bass in the MSS and REF areas appeared similar in 2003-04, but larger fish were absent from samples collected in the MNS area in 2004. Mean Wr was generally similar for fish collected in the MSS and REF areas and lower for fish collected in the MNS area. This was especially true for fish in the 150-199 mm length group.

Declines in recruitment resulting from competition with recently stocked species of fish may be impacting the largemouth bass population in Lake Norman. Recent introductions of alewives and white perch may have increased lakewide predation on largemouth bass eggs and juveniles (e.g., Kohler and Ney 1980, Madenjian et al. 2000), and resulted in the changes noted in largemouth bass abundance. A change in largemouth bass abundance may also be reflected in growth rates for this species. A decline in abundance may increase the growth rates of surviving largemouth bass while an increase in abundance may reduce growth. It is difficult to determine if introduction of the spotted bass to Lake Norman has resulted in 5-5

competition with the largemouth bass and may have also reduced growth rates and mean Wr primarily in the MNS area where spotted bass were most abundant.

In 2004, a total of 2,610 dead striped bass were collected from surveys conducted between July 1 and August 30 in Lake Norman (Table 5-5). Only 10 dead stripers were collected during a similar period in 2003 (Duke Power 2004). Most of these dead fish were collected in Zone I from July 22 through August 13. This kill was reported and investigated by the NCWRC (Waters 2004).

This die-off of striped bass was the largest ever for Lake Norman and coincided with the temporary loss of striped bass habitat near the dam in late July (see Chapter 2). As noted in Chapter 2, habitat conditions were marginally better in 2004 than 2003 when few striped bass died (Duke Power 2004). Thus, this kill was unexpected and may be related to an unusually warm May (Figure 2-2c, Chapter 2) that resulted in higher than normal epilimnetic water temperatures in June and July (Figure 2-3, Chapter 2). For example, it appears that the 26 'C isotherm (upper limit used for striped bass habitat) in July was about 5 m deeper in 2004 than in 2003.

With higher than normal epilimnetic water temperatures in Lake Norman in 2004, striped bass may have migrated earlier and deeper into the metalimnion and hypolimnion of Lake Norman than in previous years. Due to the presence of alewives as a suitable prey in the hypolimnion (see small-mesh netting results in this chapter), striped bass may have remained in the hypolimnion as microbial activity reduced metalimnetic dissolved oxygen concentrations to critical levels (< 2 mg/i). In Lake Norman, metalimnetic dissolved oxygen concentrations are normally reduced prior to hypolimnetic concentrations (Figure 2-6, Chapter 2), thus "trapping" any fish that may be in the hypolimnion at this time. As dissolved oxygen concentrations in the hypolimnion were reduced to < 2.0 mg/i by late July or early August, any striped bass "trapped" there would not have been expected to survive.

Even though similar metalimnetic and hypolimnetic dissolved oxygen conditions have been documented in Lake Norman in previous years, this was the first major kill since 1983 when

< 200 fish died (Duke Power unpublished data). We can only assume that in previous years striped bass die-offs did not occur primarily because fish were not forced into the hypolimnion in late spring or early summer due to unusually warm meteorological conditions or if they were forced deeper into the reservoir, they may not have remained there for an extended period of time due to lack of a readily available food source.

5-6

Threadfin shad and gizzard shad were the primary prey for striped bass prior to the introduction of alewives into Lake Norman in the late 1990s. Gizzard shad (Coutant 1977) and probably threadfin shad have summer temperature preferences higher than alewives (Colby 1973) and would not be expected to inhabit the hypolimnion of Lake Norman in early summer as do alewives. Without sufficient prey at these deeper depths, the striped bass would not be inclined to remain in the hypolimnion long enough to become "trapped" by natural reductions in metalimnetic dissolved oxygen concentrations. Therefore, kills of the magnitude seen in 2004 would not have been expected prior to alewives becoming established in the lake. The NCWRC did not think that the number of fish killed in 2004 significantly reduced the overall numbers of striped bass in Lake Norman (Christian Waters, personal communication).

One hundred seven striped bass were collected in November and December 2004 for age, growth and Wr evaluations (Figure 5-6). Mean total length at age was 308, 451, 519, 574, 586, 600, 590 and 647 mm at ages 0-7, respectively. Growth of Lake Norman striped bass was slow after age 3 as noted in 2003 (Duke Power 2004). Mean Wr ranged from 67 to 90 and was highest for young fish and lowest for older fish. Overall, mean Wr for all fish in 2004 was 79 and was similar to that (81) noted in 2003 (Duke Power 2004).

Duke Power and the NCWRC collected 258 crappies in 105 trap-net sets from Lake Norman in 2004. These data were summarized by Nelson and Dorsey (2005). They found that mean length, mean total length at age and mean Wr for fish collected in 2004 were similar to that noted for fish collected in comparable sampling in 2003.

A total of 82 fish were collected in small-mesh gillnetting conducted in the hypolimnion of Lake Norman in 2004 (Table 5-6). Alewife (57.3 %) and blue catfish (30.5 %) composed most of the catch. The presence of alewives in the hypolimnion of Lake Norman just prior to the striped bass kill may partially explain why striped bass became "trapped" in the hypolimnion and later died due to the lack of dissolved oxygen.

Forage fish densities in the six zones of Lake Norman ranged from 633 to 6,188 fish/ha in September 2004 (Table 5-7). Forage fish densities were highest in Zones 3 and 4, intermediate in Zone 5, and lowest in Zones 1, 2, and 6. The limited amount of available habitat for sampling (i.e., shallow water where physical damage to the transducers by collision with the bottom is a high probability) in Zone 6 complicated any discussion of fish 5-7

densities in this uppermost zone of Lake Norman. The estimated population was approximately 47.2 million fish. The lakewide population estimate in September 2004 was below values measured from 1997 to 2003 when estimates ranged from 64.3 to 91.3 million fish (Figure 5-7), but no trends were noted in zonal or lakewide population estimates for pelagic fish surveys conducted in Lake Norman from 1997 through 2004.

Purse seine sampling in 2004 indicated that the forage fish sampled by hydroacoustics were 86.6% threadfin shad, 13.2% alewives, and 0.2% gizzard shad (Table 5-8). Threadfin shad lengths primarily ranged from 36 to 85 mm while alewife lengths ranged from 56 to 85 mm.

The two length frequency distributions overlapped with a modal length between 51 and 55 mm (Figure 5-8). Results from purse seining have undergone a dramatic shift in recent years. From 1997 through 1999, purse seine samples were mostly composed of small threadfin shad (typically < 55 mm long). Alewives were first detected in 1999 in low numbers and increased to approximately 25% of the open water forage fish community in 2002 and their presence was accompanied by a concurrent wider size range of individuals with a larger modal length class. The percent contribution from alewives has declined since 2002 and was approximately 13% of the forage fish catch in 2004. However, the concurrent wider size range and larger modal length class of forage individuals has persisted.

FUTURE STUDIES The only suggested change to the fish portion of the Lake Norman Maintenance Monitoring Program is to discontinue small-mesh gillnetting for fish inhabiting Zone 1 of Lake Norman's hypolimnion.

SUMMARY

In accordance with the Lake Norman Maintenance Monitoring Program for the NPDES permit for MNS, specific fish monitoring programs were coordinated with the NCWRC and continued during 2004. Spring electrofishing indicated that 17 to 21 species of fish and 2 hybrid complexes comprised fish populations in the 3 sampling areas, and numbers and biomass of fish in 2004 were generally similar to those noted since 1993. Declines in largemouth bass numbers, which were first observed in 2000, appear to be an exception.

5-8

In 2004, considerable striped bass mortality was observed during summer in Lake Norman and this mortality appeared to be related to a combination of unique events triggered by an unusually warm May and the abundance of prey in the hypolimnion. Mean Wr for Lake Norman striped bass collected in November and December 2004 was similar to that observed previously and indicated little change in the overall condition of this fish.

Trapnetting indicated little change in the crappie populations in Lake Norman in 2003-2004.

Hydroacoustic and purse seine sampling indicated that there was a decline in the number of prey fish and a change in species composition from 2003 to 2004.

LITERATURE CITED Anderson, R. A., and R. M. Neumann. 1996. Length, weight, and associated structural indices. Pages 447-482 in B. R. Murphy and D. W. Willis, editors. Fisheries Techniques. American Fisheries Society, Bethesda, Maryland.

Brandt, S. B. 1996. Acoustic assessment of fish abundance and distribution. Pages 385-432 in B. R. Murphy and D. W. Willis, editors. Fisheries Techniques. American Fisheries Society, Bethesda, Maryland.

Devries, D. R., and R. V. Frie. 1996. Determination of age and growth. Pages 483-512 in B. R. Murphy and D. W. Willis, editors. Fisheries Techniques. American Fisheries Society, Bethesda, Maryland.

Duke Power. 2004. Lake Norman maintenance monitoring program: 2003 summary. Duke Energy Corporation, Charlotte, North Carolina.

Colby, P. J. 1973. Response of the alewives, Alosa psedoharengus, to environmental change. Pages 163-198 in W. Chavin, editor. Responses of fish to environmental changes. C. C. Thomas, Springfield, Illinois.

Coutant, C. C. 1977. Compilation of temperature preference data. Journal of the Fisheries Research Board of Canada 34:739-745.

Hayes, D. B., C. P. Ferrier, and W. W. Taylor. 1996. Active fish capture methods. Pages 193-220 in B. R. Murphy and D. W. Willis, editors. Fisheries Techniques. American Fisheries Society, Bethesda, Maryland.

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Kohler, C. C., and J. J. Ney. 1980. Piscivority in a land-locked alewife (Alosa pseudohargenus) population. Canadian Journal of Fisheries and Aquatic Sciences 37:1314-1317.

Madenjian, C. P., R. L. Knight, M. T. Bur, and J. L. Forney. 2000. Reduction in recruitment of white bass in Lake Erie after invasion of white perch. Transactions of the American Fisheries Society 129:1340-1353.

Nelson, C., and L. Dorsey. 2005. Population characteristics of black crappies in Lake Norman 2004. Survey Report, Federal Aid in Fish Restoration Project F-23-S. North Carolina Wildlife Resources Commission, Raleigh, North Carolina.

Siler, J. R. 1981. Growth of largemouth bass, bluegill, and yellow perch in Lake Norman, North Carolina--A summary of 1975 through 1979 collections. Research Report PES/81-6. Duke Power Company, Huntersville, North Carolina.

Siler, J. R., W. J. Foris, and M. C. McInerny. 1986. Spatial heterogeneity in fish parameters within a reservoir. Pages 122-136 in G. E. Hall and M. J. Van Den Avyle, editors.

Reservoir Fisheries Management: Strategies for the 80's. Reservoir Committee, Southern Division American Fisheries Society, Bethesda, Maryland.

Waters, C. 2004. Fish kill field investigation report. North Carolina Department of the Environment and Natural Resources, Division of Water Quality Environmental Science Branch, Raleigh, NC.

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Table 5-1. Common and scientific names of fish collected in Lake Norman, 2004.

Common name Scientific name Blueback herring Alosa aestivalis Alewife Alosa pseudoharengus Gizzard shad Dorosomacepedianum Threadfin shad Dorosomapetenense Goldfish Carassiusauratus Greenfin shiner Cyprinella chloristia Whitefin shiner Cyprinella nivea Common carp Cyprinus carpio Eastern silvery minnow Hybognathus regius Spottail shiner Notropis hudsonius Fathead minnow Pimephalespromelas Blue catfish Ictalurusfurcatus Channel catfish Ictaluruspunctatus Flathead catfish Pylodictis olivaris Striped mullet Mugil cephalus White perch Morone americana Striped bass Morone saxatilis Redbreast sunfish Lepomis auritus Green sunfish Lepomis cyanellus Warmouth Lepomis gulosus Bluegill Lepomis macrochirus Redear sunfish Lepomis microlophus Hybrid sunfish Lepomis hybrid Spotted bass Micropteruspunctulatus Largemouth bass Micropterussalmoides Hybrid black bass Micropterus hybrid White crappie Pomoxis annularis Black crappie Pomoxis nigromaculatus Tessellated darter Etheostoma olmstedi Yellow perch Percaflavescens 5-11

Table 5-2. Numbers and biomass of fish collected from electrofishing ten 300-m transects near Marshall Steam Station (MSS), in a reference (REF) area between MSS and McGuire Nuclear Station (MNS) and near MNS in Lake Norman, March and April 2004.

MSS REF MNS Taxa N Kg N Kg N Kg Alewife 1 0.004 47 0.329 Gizzard shad 3 1.352 1 0.635 1 0.700 Goldfish 1 1.517 Greenfin shiner 4 0.007 4 0.014 4 0.010 Whitefin shiner 68 0.415 145 0.430 110 0.252 Common carp 19 43.111 23 46.592 11 21.159 Eastern silvery minnow 2 0.007 Spottail shiner 149 1.355 52 0.410 9 0.060 Fathead minnow 1 0.001 Channel catfish 1 0.128 5 3.126 Flathead catfish 4 0.768 1 0.133 5 0.416 Striped mullet 1 2.408 White perch 1 0.424 Redbreast sunfish 523 8.898 497 7.478 288 4.863 Green sunfish 2 0.042 7 0.020 Warmouth 22 0.169 51 0.568 46 0.250 Bluegill 1,202 12.322 1,013 10.710 1,013 14.115 Redear sunfish 150 8.696 153 6.387 94 3.410 Hybrid sunfish 115 2.920 132 2.481 82 1.813 Spotted bass 27 4.079 1 0.014 59 10.691 Largemouth bass 140 56.651 87 30.071 36 11.288 Hybrid black bass 4 0.241 1 0.826 3 0.296 Black crappie 3 1.000 5 3.947 1 0.553 Tessellated darter 2 0.002 1 0.002 3 0.005 Yellow perch 6 0.043 10 0.111 Total 2,449 142.635 2,229 114.264 1,772 73.826 5-12

Table 5-3. Mean total lengths (mm) at age for largemouth bass collected from electrofishing ten transects near the Marshall Steam Station (MSS), in a reference (REF) area between MSS and McGuire Nuclear Station (MNS) and near MNS in Lake Norman, March-April 2004. Numbers of fish used to compute means are in parentheses.

Age Location 1 2 3 4 5 6 7 8 9 MSS 176(47) 309(37) 355(17) 367(14) 403(11) 417(2) 428(2) 415(2)

REF 143(47) 288(16) 364(13) 415(4) 404(3) 382(6) 379(1) 394(1)

MNS 170 (50) 276 (18) 335 (9) 370 (14) 356 (1) 414 (1)

Weighted mean 163 296 353 374 403 387 428 406 394 Table 5-4. Mean total length (mm) at age for largemouth bass collected from an area near the Marshall Steam Station (MSS), from an area (REF) between MSS and the McGuire Nuclear Station (MNS), and from an area nearMNS. Data from 1971-78, 1993-94, and 2003 are from Siler (1981), Duke Power unpublished data, and Duke Power (2004), respectively.

Age Location and year 1 2 3 4 MSS 1974-78 170 266 310 377 MSS 1993 170 277 314 338 MSS 1994 164 273 308 332 MSS 2003 216 317 349 378 MSS 2004 176 309 355 367 REF 1993 157 242 279 330 REF 1994 155 279 326 344 REF 2003 139 296 358 390 REF 2004 143 288 364 415 MNS 1971-78 134 257 325 376 MNS 1993 176 256 316 334 MNS 1994 169 256 298 347 MNS 2003 197 315 248 389 MNS 2004 170 276 335 370 5-13

Table 5-5. Dead or dying striped bass observed in Lake Norman, July-August 2004.

Dat Number Zon Range in total length 1-Jul 3 1 522-1 3 565 1 4 589 6-Jul 2 4 400-9-Jul 1 1 592 1 2 575 2 4 464-15-Jul 2 1 558-1 3 535 3 4 508-22-Jul 10 1 512-2 2 520-7 3 429-4 4 480-27-Jul 22 1 423-10 3 526-29-Jul 91 1 450-1 2 545 30-Jul 204 1 441-1 3 540 1 4 570 31-Jul 90 1 425-2 2 455-1 3 515 2 4 558-1- 72 1 466-2- 112 1 462-5-14

Table 5-5. Continued.

Date Number Zone Range in total length (mm) 3-Aug 86 l 464-623 1 4 510 4-Aug 191 1 449-819 5-Aug 200 1 435-650 2 3 523-540 1 4 503 6-Aug 206 1 1 2 3 3 3 4

  • 7-Aug 318 1 8-Aug 479 1 9-Aug 166 1 10-Aug 197 1
  • 4 2 5 3 1 4 11-Aug 53 1 12-Aug 22 1 13-Aug 11 1 20-Aug 5 1 450-500 1 2 510 3 3 500-595 1 4 540 30-Aug 1 4 600
  • Length data not collected 5-15

Table 5-6. Numbers of fish caught in small-mesh gill nets set in Lake Norman's hypolimnion, July 2004.

Depth and percent compostion Species Upper % Bottom % Combined %

Blueback herring 5 7.6 1 6.2 6 7.3 Alewife 43 65.2 4 25.0 47 57.3 Gizzard shad 1 1.5 0 1 1.2 Blue catfish 14 21.2 11 68.8 25 30.5 White perch 1 1.5 0 1 1.2 Striped bass 2 3.0 0 2 2.4 Table 5-7. Prey fish densities (N/ha) and population estimates from hydroacoustic surveys of Lake Norman, September 2004.

Zone Density Population estimate 1 1,811 4,130,891 2 1,752 5,399,839 3 6,188 21,382,758 4 5,717 7,037,627 5 4,235. 8,918,910 6 633 302,574 Lakewide 47,172,599 95% Lower confidence interval 43,699,683 95% Upper confidence interval 50,645,514 5-16

Table 5-8. Numbers (N), species composition and modal lengths (mm) of prey fish collected in purse seine samples collected in Lake Norman during late summer or fall, 1997-2004.

Species composition Threadfin shad modal Year N Threadfin Gizzard Alewife length class 1997 6,711 99.99% 0.01% 0.00% 41-45 1998 5,723 99.95% 0.05% 0.00% 41-45 1999 5,404 99.26% 0.26% 0.48% 36-40 2000 4,265 87.40% 0.22% 12.37% 51-55 2001 9,652 76.47% 0.01% 23.52% 56-60 2002 10,134 74.96% 0.00% 25.04% 41-45 2003 33,660 82.59% 0.14% 17.27% 46-50 2004 21,158 86.55% 0.24% 13.20% 51-55 5-17

Legend

  • Spring Electrofishing Locations a Fsh HealthAssessmentLocations A Purse Seine Locations Yrone 5 41 r Zone 2 Zone 3

. j-) Q

+/-+

a' Zone 1 Cowans Ford -

0 1 2 3 Miles Hydro McGuire Nuclear Station Figure 5-1. Sampling zones in Lake Norman.

CC?-

5-18

3500 3000- l DMSS U REF 2500- UMNS 20 - 99 450 z

1000 -

500 -

0 1993 1994 1995 1996 1997 1999 2000 2001 2002 2003 2004 Years 450-400-

  • MSS 350-OMNS 300 250 200 850 150-0 1993 1994 1995 1996 1997 1999 2000 2001 2002 2003 2004 Years Figure 5-2. Numbers and biomass of fish collected from electrofishing ten 300-m transects near Marshall Steam Station (MSS), a reference (REF) area between MSS and and McGuire Nuclear Station (MNS), and near MNS in Lake Norman, 1993-2004.

5-19

300 250 -* REFI 03MNS 200-2 60 -*5 z

100 50-0 1993 1994 1995 1996 1997 1999 2000 2001 2002 2003 2004 Years 70-IDMSS 60 - oREF DMNS 50-t2 30 30 20 1 2 10 0

1993 1994 1995 1996 1997 1999 2000 2001 2002 2003 2004 Years Figure 5-3. Numbers and biomass of largemouth bass collected from electrofishing ten 300-rn transects near Marshall Steam Station (MSS), a reference (REF) area between MSS and McGuire Nuclear Station (MNS), and near MNS in Lake Norman, 1993-2004.

5-20

40 35- L1MSS

25-

~20-z 15 10 -

5-0

<150 150-199 200-249 250-299 300-349 350-399 400449 450-4499 Length groups (mm)

Figure 5-4. Size distribution of largemouth bass collected from electrofishing ten 300-m transects near Marshall Steam Station (MSS), in a reference (REF) area between MSS and McGuire Nuclear Station (MNS) and near MNS in Lake Norman, 2004.

5-21

100 90 80 70 60-SIMSS 50 n U REF 40-30 20 -

10 0 - --- - i 150-199 200-249 250-299 300-349 350-399 400-449 450-499 Length groups (mm)

Figure 5-5. Mean relative weight (Wr) for largemnouth bass collected from electrofishing ten transects near Marshall Steam Station (MSS), in a reference (REF) area between MSS and McGuire Nuclear Station (MNS) and near MNS in Lake Norman, 2004.

5-22

700 100

[CTL Wr t -90 600 80 500 N70 60 400 1 50~

300 -

40 200 30 2020 1000 1010 0 _ -- -- 0 0 1 2 3 4 5 6 7 Age Figure 5-6. Mean total length and mean relative weight (Wr) for striped bass collected from Lake Norman, November-December 2004. Number of fish associated with the mean length are inside the bars.

5-23

100.0 - -- -I

-+-2 90.0 - a 3 4

80.0 - X 5

-+-6 70.0 -

- Lakewide 0

= 60.0-

= 50.0-0 40.0-z 30.0 -

20.0 -

10.0 -

- *i_ ,

0.0

  • 1997 1998 1999 2000 2001 2002 2003 2004 Years Figure 5-7. Zonal and lakewide population estimates of pelagic fish in Lake Norman.

5-24

180 160 59 TFS 140 L GZS 0 ALE 120 100 Z80 60-40 20 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 Length groups (mm)

Figure 5-8. Size distributions of threadfin shad (TFS), gizzard shad (GZS) and alewives (ALE) collected in purse seine surveys of Lake Norman, 2004.

5-25