ML17261A197
| ML17261A197 | |
| Person / Time | |
|---|---|
| Site: | Susquehanna  |
| Issue date: | 09/13/2017 |
| From: | Anthony Williams Tennessee Valley Authority |
| To: | Document Control Desk, Office of Nuclear Reactor Regulation, State of TN, Chattanooga Environmental Field Office, Division of Water Pollution Control |
| References | |
| TN0026450 | |
| Download: ML17261A197 (101) | |
Text
{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 September 13, 2017 Chattanooga Environmental Field Office Division of Water Pollution Control 1301 Riverfront Parkway, #206 Chattanooga, Tennessee 37402-2013 TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR August 2017 Enclosed is the August 2017 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period. The final report for toxicity testing conducted July 23 - 28, 2017 is enclosed along with the corrected July DMR for 101 T. If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843~6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of iny knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and im ri onment for knowing violations. on . illiams Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555
PERMIITEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name _ _!VA-SEQUO~~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres,L _f.Q....BOX 20QQ_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _(lNTEROFFICE OPS-5N-SQ.Nl _ _ _ _ _ _ _ _ TN0026450 101 G F- FINAL _ _ _ _§ODDY - DAISYJ.N_]73B1.._ _ _ _ _ _ _ _ _ DIFFUSER DISCHARGE Fa@_~A-SEQUO~HNUCLEAR&NL _ _ _ _ _ EFFLUENT LocatiO.!L ...!:!AMILTOJi.COUNTY_ - - - - - - - - - - ATIN:Millicent Garland Dy 31
*** NO DISCHARGE D ***
NOTE: Read instructions before completinq this form. IX PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 42.1 31 / 31 RCORDR MEASUREMENT ** 04 0 CENTIGRADE 00010 1 0 *PERMIT *******"' *"'****** **** ******** ******** Req. Mon. DEG.C. CONTI CALCTD REQUIREMENT* EFFLUENT GROSS
.. DAiLYMAX NUOUS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 30.1 31/31 MOD ELD MEASUREMENT 04 0 CENTIGRADE 00010 z 0 PERMIT ******** ******** **** ******** *"'****** 30.5 DEG.C. CONTI CALCTD REQUIREMENT INSTREAM MONITORING DAILYMX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** 2.3 31/31 CALCTD MEASUREMENT ** 04 0 UPSTRM DEG.C 00016 1 s PERMIT ****"*"'* ******** fritn\:fr
******** *****"'** 3.0 DEG.C. CONTI 'CALCTD REQUIREMENT EFFLUENT GROSS DAILYMX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ********* ******** ******** ******** 31 / 31 RCORDR
. 03 ** 0 TREATMENT PLANT MEASUREMENT 11!'!1 50050 1 0 PERMIT .. **"'****** Req.Mon. MGD ""'****** *******"' ******** **** CONTI RCORDR REQUIREMENT
- EFFLUENT GROSS DAILY MAX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** 31/31 CALCTD MEASUREMENT 1799 03 ******** 03 0 TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. ******** MGD *******"' ******** ******** MGD CONTI* CALCTD REQUIREMENT EFFLUENT GROSS VALUE MO.AVG NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** 0.017 0.043 0 25 / 31 GRAB MEASUREMENT ** 19 50060 1 0 PERMIT ******** ******** **** ******** 0.1 0.1 MG/L FIVE PER CALCTD REQUIREMENT EFFLUENT GROSS VALUE MO AVG DAILY MAX WEEK TEMPERATURE - C, RATE OF SAMPLE ******** 0.3 ******** ******** 0 31/31 CALCTD CHANGE MEASUREMENT 62 **
82234 1 0 PERMIT REQUIREMENT
******** 2.0 DEG C/HR
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******* **** CONTI CALCTD l
EFFLUENT GROSS DAILY MX / NUOUS
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all aUachmenls were prepared under direction or supervision in accordance with a system designed to assure that qualified _L' TELEPHONE DATE Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inquiry of I e person or persons who manage the system, or those persons directly responsible for gathe pg ite Vice President the information, the information submitted is , to the best of my knowledge and belief, true, *~ 423 843-7001 17 09 11 Site Vice President accurate,and complete. I am aware that there are significant penalties for submiUing false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following injections occurred: Spectrus BD 1500 (max calc. 0.03 mg/L, limit --0.05 mg/L) and Spectrus CT 1300 (max calc. was 0.025 mg/L, limit -- 0.05 mg/L). EPA Form 3320-1 (REV 3/99) Previous editions may be used . Paae 1*of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~-~~-SEQUO~~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres,L _.E.Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ .J!.NTEROFFICE OP§.:§.N-SQ!il _ _ _ _ _ _ _ _ TN0026450 101 T F- FINAL
---~ODDY-DAISYJ.ij73BL _ _ _ _ _ _ _ _ BIOMONITORING FOR OUTFALL 101 PERMIT NUMBER DISCHARGE NUMBER Faclfily_ ..JYA - SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _
r~RING ff:.'~ MO Locatio..!L _l:!AMILTOli.COUNTY.._ _ _ _ _ _ _ _ _ _ _ EFFLUENT Y~;R MO , DAY ATIN:Millicent Garland From 1 J 08 _ 01_ To I 17 I 08 / 31
*** NO DISCHARGE D ***
x NOTE: Read instructions before completini:r this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ******** Monitoring ******** MEASUREMENT
******** ** ******** 23 CERIODAPHNIA Not Required TRP3B 1 0 .PERMIT
....... 42.8 ********* ******** PERCENT SEMI COMPOS
. REQUIREMENT EFFLUENT GROSS MINIMUM* ANNUAL IC25.STATRE 7DAY CHR SAMPLE ******** Monitoring ********
MEASUREMENT
******** ** ******** 23 PIMEPHALES Not Required TRP6C 1 0 PERMIT
******** ******'"" **** .. 42.8 *******" ******'"" PERCENT SEMI COMPOS R.EQ(JJREMENT EFFLUENT GROSS MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Anthony L Williams Site Vice President REQUIREMENT
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direction or supervision in accordance with a system designed to assure that ,,_oo personnel properly gather and evaluate the information submitted. Based on my inquiry oft
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v-- )* SIGNATURE OF PRINCIPAL EXECUTIVE 423 TELEPHONE I 843-7001 17 DATE 09 11 TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in August 2017. EPA Form 3320-1 (REV 3199) Previous editions may be used Pam'! 1 nf 1
. PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name _ _!VA-SEQUO~~UCLEA~LANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Addres,L _E.Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _(lNTEROFFICE OPS-5N-SQN) _ _ _ _ _ _ _ _ TN0026450 103 G F- FINAL _ _ _ __.§.ODDY - DAISY,_I.N_l73BL_ _ _ _ _ _ _ _ _ LOW VOL. WASTE TREATMENT POND Facl!l!Y_ .JYA - SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ EFFLUENT LocaliO.!L ...!:!AMILTOJi.COUNTY_ - - - - - _ _:_ _ - - From Dy
*** NO DISCHARGE D ...
ATTN:Millicent Garland 31 NOTE: Read instructions before completin~ this Form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
' EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH SAMPLE
******** ******** 7.0 ******** 7.4 0 6 / 31 GRAB MEASUREMENT ** 12 00400 1 0 PERMIT ******'** ******** ** 6.0 . ******** 9.0 SU ONCE/ GRAB REQUIREMENT EFFLUENT GROSS MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ********* ******** 8.0 8.0 0 1 / 31 GRAB MEASUREMENT ** 19 00530 1 0 PERMIT ******** ******** ** ******** 30.0 100.0 MG/L ONCE/ GRAB REQUIREMENT EFFLUENT GROSS MO AVG DAILY MX MONTH OIL AND GREASE SAMPLE ******** ******** ******** <5.0 <5.0 0 1/ 31 GRAB MEASUREMENT
** 19 00556 1 0 PERMIT ******** ******** ** ******** 15.0 ~0.0 MG/L ONCE/* GRAB REQUIREMENT EFFLUENT GROSS MO AVG DAILYMX MONTH SAMPLE 0.887 ******** ******** ******** 5 / 31 IN STAN FLOW, IN CONDUIT OR THRU MEASUREMENT 0.779 03 ** 0 TREATMENT PLANT 50050 1 0 PERMIT REQUIREMENT Req. Mon. Req. Mon MGD ****O** ******** ******** .. ONCE/ *INSTAN EFFLUENT GROSS MO AVG DAILYMX WEEK SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT v---*~
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inquiry of he person or persons who manage the system, or those_ persons directly responsible for gal ering 1te Vice President the information, the information submitted is , to the best of my knowledge and belief, true, 423 843-7001 17 09 11 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~~-SEQUO~~UCLEARPLANT ___ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres.L _f.Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ .J!.NTEROFFICE OPS-5N-SQ.Nl _ _ _ _ _ _ _ _ TN0026450 110 G F - FINAL
---~DDY-DAISU~738L _ _ _ _ _ _ _ _ RECYCLED COOLING WATER Facjfily_ __IYA -_fil:Q!.JOYAH NUCLEAR PLANT_ _ _ _ _ _
Locatio.!L .J:!AMILTOl:!...COUNTY __________ _ EFFLUENT ' From
*** NO DISCHARGE Ixx I ATTN:Millicent Garland NOTE: Read instructions before completinr:i this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO, FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** MEASUREMENT
******** ** 04 CENTIGRADE 00010 1 0 PERMIT ***"'**** ******** ** ******** *"'****** REPORT DEGC CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE DAILY MX uous TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ********
MEASUREMENT ** 04 CENTIGRADE 00010 z 0 PERMIT REQUIREMENT
******** !\'.*****"" .. **"'***** ******** 30.5 DEGC CONTIN CALCTD INSTREAM MONITORING - DAILYMX uous TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ********
MEASUREMENT ** 04 UPSTRM DEG.C 00016 1 0 PERMIT *****"'** *"'****** ** ****II:*** ******** 5 DEGC CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE DAILY*MX uous FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** ******** MEASUREMENT 03 ** TREATMENT PLANT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ******** ******** ** CONTIN RCORDR REQUIREMENT EFFLUENT GROSS VALUE DAILY MX uous CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** MEASUREMENT ** 19 50060 1 0
- PERMIT ******** ******** ** ***"'*"** MG/L Five per CALCTD*
0.1 0.1 REQUIREMENT EFFLUENT GROSS VALUE MO AVG DAILYMX Week TEMPERATURE - C, RATE OF SAMPLE ******** ******** ******** ******** MEASUREMENT
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CHANGE 82234 1 0 PERMIT ******** 2 DEGC ******!\'.* ******** ******** ** CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE DAILYMX uous SAMPLE MEASUREMENT PERMIT t REQUIREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under
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TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry oft e S~ Vice President Anthony L. Williams person or persons who manage the system, or those persons directly responsible for gathe ing the information, the information submitted is , to the best of my knowledge and belief, true, 423 843-7001 17 09 11 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~-~~-SEQUO~~u~~ARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMRJ OMB No. 2040-0004 (SUBR 01) Addres_L _f.~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ -11.NTEROFFICE OPS-5N-SQNl _ _ _ _ _ _ _ _ TN0026450 F - FINAL _ _ _ _§_ODDY - DAISY....INJ7364_ _ _ _ _ _ _ _ _ RECYCLED COOLING WATER Faclfily_ __IVA-_fil:QUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!L .J:!AMILTOl:!_COUNTY_ _ _ _ _ _ _ _ _ _ _ EFFLUENT From
*** NO DISCHARGE Ixx I ***
ATTN:Millicent Garland NOTE: Read instructions before completini:i this form. PARAMETER NO. FREQUENCY SAMPLE IX QUANTITY OR LOADING QUALITY OR CONCENTRATION EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** MEASUREMENT
******** ** 23 CERIODAPHNIA TRP38 1 0 0 PERMIT ******** ******** **** 42.8 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ********
PIMEPHALES MEASUREMENT ** 23 TRP6C 1 0 0 PERMIT ******** ******** **** 42.8 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT ' - REQUIREMENT SAMPLE , MEASUREME.NT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
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D NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law !hat !his document and all aUachmenls were prepared under my Anthony L. Williams direction or supervision in accordance wilh a system designed lo assure Iha! qualified personnel properly gather and evaluate lhe information submiUed. Based on my inquiry of Iha i(v ~. TELEPHONE DATE person or persons who manage the system, or those persons directly responsible for gathering ite Vice President
!he information, !he information submiUed is , lo lhe bes! of my knowledge and belief, true, ~
423 843-7001 17 09 11 Site Vice President accurate, and complete. I am aware Iha! !here are significant penalties for submilling false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including !he possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~~2~-SEQUO~~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres.L _.e..Q,_BOX 20Q.Q._ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _llNTEROFFICE OPS-5N-SQ.Nl _ _ _ _ _ _ _ _ TN0026450 118 G F- FINAL _ _ _ _§ODDY - DAISYJ.N_]73B1._ _ _ _ _ _ _ _ _ WASTEWATER & STORM WATER Facl!i!Y_.JYA-SEQ110YAH NUCLEAR PLANT_ _ _ _ _ _ Locati~.JiAMILTO~OUNTY._ _ _ _ _ _ _ _ _ _ _ EFFLUENT From EAR 17
*** NO DISCHARGE Ixx I ***
ATIN:Millicent Garland ~-~--~--~ NOTE: Read instructions before completinq this form.
>< NO. FREQUENCY SAMPLE PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION EX OF TYPE
.. ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO) SAMPLE ******** ********
MEASUREMENT
******** ** ******** 19 00300 1 0 PERMIT *****""* ******** . **** 2 *"'****** ******** MG/L TWICE/ GRAB REQUIREMENT EFFLUENT GROSS .. MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** ********
MEASUREMENT ** 19 00530 1 0 PERMIT ******** ******** **** ******** *******"' 100 MG/L TWICE/ GRAB REQUIREMENT EFFLUENT GROSS DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE ******** ******** ******** M~ASUREMENT
******** ** 25 00545 1 0 PERMIT ******** ******** **** ******** ******** 1 MUL ONCE/ GRAB REQUIREMENT EFFLUENT GROSS DAILY MX MONTH
- FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ********
MEASUREMENT 03 ** TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ******** ******** ********
- ONCE/ ESTIMA REQUIREMENT EFFLUENT GROSS MO AVG DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT Pl;RMIT REQUIREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law Jhat lhis document and all attachments were prepared under my / TELEPHONE DATE direction or supervision in accordance with a system designed Jo assure that qualified g( Anthony L. Williams personnel properly galher and evaluate Jhe information submitted. Based on my inquiry of lhe person or persons who manage Jhe system, or those persons directly responsible for galhering - - ~ite Vice President r the information, the informalion submitted is , to the besl of my knowledge and belief, true, 423 843-7001 17 09 11 Site Vice President accurate, and complete. I am aware that lhere are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfaU. No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name _ _!VA~EQUOYA~UCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres,L _E.~BOX 20QQ... _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _(iNTEROFFICE OPS-5N-SQ.Nl _ _ _ _ _ _ _ _ TN0026450 101 T F- FINAL _ _ _ 20DDY-DAISY..lij73BL _ _ _ _ _ _ _ _ BIOMONITORING FOR OUTFALL 101 Facl!i!Y_....I.VA-.§EQUOYAH NUCLEAR PLANT_ _ _ _ _ _ LocatiOJL _!:!AMIL TQt!_COUNTL_ _ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN:Millicent Garland DA 31
*** NO DISCHARGE D ...
NOTE: Read instructions before completinQ this form.
>< NO. FREQUENCY SAMPLE PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ******** ******** >100.0 ******** ******** 0 3/180 COMPOS MEASUREMENT ** 23 CERIODAPHNIA TRP38 1 0 PERMIT ******** ******** **** 42.8 ****"'*** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** >100.0 ******** ******** 0 3 / 180 COMPOS MEASUREMENT ** 23 PIMEPHALES TRP6C 1 0 PERMIT ******** ******** **** 42.8 **"'***** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT
'PERMIT REQUIREMENT SAMP.LE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT et /
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~idenf NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1Certify under penalty of law that this document and all altachments were prepared under TELEPHONE DATE direction or supervision in accordance with a system designed to assure !hat qualified Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inquiry of e person or persons who manage the system, or those persons directly responsible for galhe 'ng the information, the information submilled is , to the best of my knowledge and belief, true, 423 843-7001 17 09 07 Site Vice President accurate, and complete. I am aware !hat there are significant penalties for submilling false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including !he possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was sampled July 23 - 28, 2017. The final report will be included in the August DMR. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
TENNESSEE VALLEY AUTHORITY. TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE
SUMMARY
Report Date: August 11. 2017
- 1. Facility I Discharger: Sequoyah Nuclear Plant I TVA
. 2. County I State: Hamilton I Tennessee
- 3. NPDES Permit#: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant *
- 5. Design Flow (MGD): 1.579
- 6. Receiving Stream: Tennessee River CTRM 483.6)
- 7. lQlO: 3,491
- 8. Outfall Tested: 101
- 9. Dates Sampled: July 23 -28. 2017
- 10. Average Flow on Days Sampled (MGD): 1910, 1913, 1781
- 11. Pertinent Site Conditions: Production I operation data will be provided upon request.
- 12. Test Dates: July 25 -August 01, 2017
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia) *
- 15. Concentrations Tested(%):
Pimephales promelas: UV treated Outfall 101: 10.7. 2L4, 42.8. 85.6, 100 UV treated Intake: 100 Ceriodaphnia dubia: Non-treated Outfall 101: 10.7, 21.4; 42.8, 85.6, 100 Non-treated Intake: t 00
- 16. Permit Limit Endpoint(%): Outfall 101: ICii = 42.8%
- 17. Test Results: Outfall 101: Pimephales promelas: ICii > 100%
Ceriodaphnia dubia: ICii > 100% Page 1of93
- 18. Facility
Contact:
Millicent Garland Phone#: (423) 843-6714
- 19. Consulting I Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone#: (828) 350-9364
- 21. TVA
Contact:
Donald W. Snodgrass Phone#: (256) 386-2787
- 22. Notes: Exposures to samples collected July 23 -28, 2017 from Outfall 101 resulted in no toxic effects to fathead minnbws or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
j Page 2 of93
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall' 10 l, Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) .Temp. TRC* Time (ET) ID Collected Received (DC) - (m£?:/L) Last Used Bv 101 07-23-17 0750 to 07-25-17 0926 I 07-24-17 1235 2.1 <0.10 07-24-17 0650 07-26-17 0827 07-23-17 0810 to 07-25-17 0926 Intake 07-24-17 1235 0.6 <0.10 07-24-17 0710 07-26-17 0827 07-25-17 0720 to 07.;27-17 0847 101 07-26-17 1232 2.6 <0.10 07-26-17 0620 07-28~17 0910 07-25-17 0735 to 07-27-17 0847 Intake 07-26-17 1232 2.7 <0.10 07-26-17 0635 07-28-17 0910 07-29-17 0930 07-27-17 0735 to 1.7, l.9t 101 07-28-17 1234 <0.10 07-30-17 0900 07-28-17 0635 07-31-17 0908 07-29-17 0930 07-27-17 0745 to Intake 07-28-17. 1234 0.9 <0.10 07-30-17 0900 07-28-17 0645 07-31-:17 0908
*TRC = Total Residual Chlorme tsamples were collected in two 2.5 gallon cubitainers. Temperature wa.S measured in.each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were wam1.ed to test temperature (25 .0 +/- 1.0°C) in a warm water bath. .
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.
- Page3 of93
Pimephales promelas Ceriodaphnia dubia Test Organisms:
- 1. Source: In-house Cultures In..:house Cultures
- 2. Age: < 24~hours old . < 24-hours old Test Method Summary:
- 1. Test Conditions: Static. Renewal Static. Renewal
- 2. Test Duration: Until at least 60% of control females have 3 broods
- 3. Control I Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: +/- lQ
- 5. Organisms per Replicate: 10 l
- 6. Test Initiation: (Date/Time): 07-25-17 0835 ET 07-25-17 0926 ET
- 7. Test Termination: (Date/Time): 08-01-17 0800 ET 08-01-17 0830 ET
- 8. Test Temperature: Outfall 101: Mean= 24.7°C Mean = 24.9°C C24.2-25.2°C) (24.6 - 25 .2°C)
- 9. Physical I Chemical Measurements: Alkalinity. hardness. total residual chlorine, and conductivity were measured at the laboratory in each *100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCafo version 5.0 I statistical software (Tidepool Scientific Software, McKinneyville, CA).
Page4 of93.
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.*
(Genus species) * * (Type /Duration)* Conducted July 25 - August 0 l, 2017 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used .:. :. davs) (%Effluent) 1 2 3 4 5 6 7 Control, 100 100 100 100 100 100 100 UV-treated 10.7% 100 100 100 too 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 . 85.6% 100 100 100 100 100 100 100
. 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Control, 100 100 100 100 100 100 98 Non-treated Mean Dry Weight (mg)
Test Solutions (replicate number) (%Effluent) 1 2 3 4 Mean Control, 0.588 .0.556 0.528 0.602 0.569 UV-treated 10.7% 0.612 0.648 0.585 0.650' 0.624 21.4% 0.649 0;584 0.603 0.576 0.603 42.8% 0.664 0.609 0.659 0.655 0.647 85.6% 0.576 0.606 0.640. 0.569 0.598 100.0% 0.617' 0.570 0.568 .. 0;540 0.574 Intake 0.530 0.592 *. 0.557 0.555 0.559 Control, 0.660 0.605 0.560 0.636 0.615 Non-treated IC25 Value: > 100% Calculated TO Estimates:.,
< 1.0 TUc*
Permit Limit: 42.8% Permit Limit: 2.3 TUc
- 95% Confidence Limits:
. Upper Limit: NA*
Lower Limit: NA
*TUa = 100/LCso: TUc = 100/IC2s Page 5 of93
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a
- Ceriodaphnia dubia Chronic/ 7-day ToxiCitv Test.
(Genus species) * (Type I Duration) Conducted July 25 ,- August 01. 2017 using effluent from Outfall 101. Percent Surviving Test . (time interval used- days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 *.100 100 100* . 100 100 10.7% 100 100 100
- 100 . 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Reproduction (#young/female/7 days)
Test Solutions Data (replicate number) (%Effluent) I 2 3 4 5 6 7 8. 9 10 Mean Control 28 30 27 30 29 32 30 31 30 30 29.7 I 10.7% 30 35 32 31 34 33 33 33 35 32 32.8 21.4% 32 38 33 30 34 35 35 34 35 34 34.0 42.8% 36 37 36 36 36 34 34 36 31 34 35.0 85.6% 36 .37 35 38 38 37 35 36 32 38 . 36.2 100.0% 40 38 38 38 .34 39 36 41 37 38 37.9 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc*
- Permit Limit: 42.8%
Permit Limit: 2.3 TUc 95% Confidence Limits:*. Upper Limit: NA Lower Limit: NA
*TUa = 100/LCso: TUc = 100/ IC2s Page 6 of93
TOXICITY TEST RE SULTS (see Appendix C for Bench Sheets).
- 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type I Duration) Conducted July 25 -August 01. 2017 using water from Intake Percent Surviving *. Test (time intenral used .c.. days) Solutions *2 1 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100. 100 100 100 100 Reproduction: (#young/female/7 days) Test Solutions Data (replicate number) (%Effluent) l 2 3 4 5 6 7 8 9 10 Mean Control 30 28 30 27 30 29 30. .-29 29 31 29.3 Intake 32 38 35 32 '35 31 29 .34 32 34 33.2 IC2s Value: > 100% Calculated TU Estimates: <LO TUc* Permit Limit: NIA Permit Limit: N/A 95% Confidence Limits:* Upper Limit: NA Lower Limit: *NA
*TUa = 100/LCso: TUc =.100IIC2.s .
. 1*-:. '.
REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) *
- Species Date Time.* *Duration Toxicant Results (IC2s)
Pimephales promelas July 11 - 18, 2017 0810 7. days KCl 0.75 g/L Ceriodaphnia dubia July 11-18, 2017 . 0915 7-days NaCl 1.06 g/L Page 7 of93
~ PHYSICAUCHEMICAL
SUMMARY
CQ Water Chemi'ltry Mean Values and Ranges fur UV-treated Pimephales promelas and Non:treated Ceriodaphnia dubia, Sequoyah Nuclear Plant (SQN), EfHuent Outfull IO 1 and Intake CD perfunnidJuly25-August01, 2017. co 0 cc Test Sample ID Temnerature ("C) Dissolved Oxygen (mg/L) pH(S.U.) Conductance Alkalinity Hardness *Total Residual CA) Initial Final Initial Final Initial Final (µmhos/cm) (mg/L CaC03) (mg/L CaC03) Chlorine (mg/L)
. Control, 24.7 24.5 7.7 7.4 7.94 7.61 317 59 86 -
Non-treated 24.6 - 24.8 24.3 - 24.7 7.6 - 7.8 7.0 - 8.1 7.77 - 8.11 7.46 - 7.88 308 - 324 58 - 61 85 - 87 - - Control, 24.8 24.6 7.8 7.3 7.94 7.61 310 59 84 - UV-treated 24.7 - 24.9 24.3 - 25.0 7.6 - 8.0 7.0 - 7.8 7.86 - 8.03 7.48 - 7.87 301 - 315 58 - 59 83 - 87 - -
.s 10.7%
24.9 24.5 7.9 7.2 7.94 7.57 303 - - - ii 24.8 - 25.0 24.2 - 24.8 7.6 - 8.0 6.8 - 7.8 7.86 - 8.03 7.39 - 7.83 294 - 310 - - - - - -
~ 21.4%
24.9 24.5 7.9 7.2 7.95 7.58 291 - - - c:i. 24.8 - 25.0 24.2 - 24.8 7.6 - 8.0 6.7 - 7.8 7.87 - 8.04 7.40 - 7.83 282 - 300 - - - - - -
-l!l
.:: 42.8% .
24.9 24.5 7.9 7.1 7.95 7.56 264 - - - 24.8 - 25. l 24.4 - 24.7 7.6 - . 8.1 6.6 - 7.9 7.87 - 8.05 7.43 - 7.88 257 - 210 - - - . - - - l 85.6% 25.0 24.6 8.0 6.9 7.95 7.49 213 - - - Q, 24.9 - 25.1 24.4 - 24.7 7.6 - 8.1 5.7 - 8.0 7.87 - 8.05 7.32 - 7.85 206 - 216 - - - - - - 25.0 24.6 8.0 6.7 7.93 7.47 195 70 76 <0.10 100% 24.9 - 25.1 24.3 - 24.8 7.7 - 8.2 5.7 - 8.0 7.81 - 8.05 7.30 - 7.85 189 - 203 69 - 71 73 - 77 <0.10 - <0.10 25.0 24.6 8.0 7.1 7.94 7.54 191 71 80 .<0.10 Intake 24.7 - 25.2 -24.5 - 24.8 7.7 - 8.2 6.5 - 8.0 7.84 - 8.05 7.36 - 7.83 185 - 195 71 - 71 73 - 89 <0.10 - . <0.10 Control, 24.7 25.0 7.7 7.8 7.94 7.91 317 59 86 - Non-treated 24.6 - 24.8 24.8 - 25.2 7.6 - 7.8 7.6 - 8.0 7.77 - 8.11 7.83 - 7.99 308 - 324 58 - 61 85 - 87 - - 10.7% 24.8 24.9 7.6 7.8 7.96 7.89 303 -* - -
.s 24.6 - 24.9 24.7 - 25.2 7.6 - 7.7 7.6 - 8.0 7.84 - 8.10 7.80 - 7.97 292 - 317 - - - - - -
.ij 21.4%
24.8 24.9 7.7 7.8 7.96 7.89 295 - - -
.s;::
24.6 - 25.0 24.7 - 25.2 7.6 - 7.8 7.6 - 8.0 7.84 - 8.10 7.79 - 7.97 280 - 304 - - - - - -
.:: 42.8%
24.8 24.9 7.7 7.8 7.96 7.88 267 - - - er 24.6 - 25.0 24.7 - 25.1 7.6 - 7.9 7.6 - 8.0 7.85 - 8.11 7.78 - 7.95 254 - 272 -. - - - - -
*~ 85.6%
24.8 24.9 7:7 7.8 7.95 7.87 213 - - - a 24.7 - 25.0 24.8 - 25.2 7.6 - 7.9 7.6 - 8.0 7.85 - 8.10 7.77 - 7.95 207
. 7.86
- 218 - - - - - -
24.9 24.9 7.8 7.9 7.94 196 70 76 <0.10 100% 24.7 - 25.1 24.8 - 25.l 7.6 - 8.0 7.7 - 8.1 7.86 - 8:08 7.76 - 7.95 190 - 201 69 - 71 73 - 77 <0.10. - <0.10 24.9 25.0 7.8 7.9 7.95 7.91 192 70 80 <0.10 Intake 24.6 - 25.1 24.9 - 25.1 7.5 - 8.1 7.7 - 8.1 7.86 - 8.09 7.79 - 8.01 187 - 196 69 - 71 73 - 89 <0.10 - <0.10
*Note: Total residual chlorine was perfonned on non-treated Outfall 101 and Intake samples.
Overall temperature ("C) Average Minimum Maximum Pimephales promelas 24.7 24.2 25.2 Ceriodaphnia dubia 24.9 24.6 25.2
SUMMARY
I CONCLUSIONS Exposures to samples collected July 23 - 28, 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
- Page 9 of93
*Appendix A ADDITIONAL TOXICITY TEST INFORMATION *
SUMMARY
OF METHODS 1.. Pimephales proinelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastiC disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels co,nsisted of 30-mL
. polypropylene cups, each containing 15-mL oftest solution.
DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas
- Samples used in the fathead minnow test were exposed to UV light for two minutes prior to introduction of test organisms. UV treatment is used to control interference of fish pathogens. This treatment method was approved on November 23, 2015 by the State of
- Tennessee in *a letter from Jessica Murphy to Terry Cheek, Senior Manager of TVA Water Permits, Compliance, and Monitoring.
- 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None Page 10 of93
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pert~ining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B-2010.
- 4. Dissolved oxygen was measured by SM 4500-0 G-2011.
- 5. The pH was measured by SM 4500-H+ B-2011.
- 6. Conductance was measured by SM 2510 B-2011.
- 7. Alkalinity was measured by SM 2320 B".2011.
- 8. Total hardness was measured by SM 2340 C-2011.
- 9. Total residual chlorine was measured by ORION 97-70-1977.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCL
- 2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 11of93
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEP A. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 2012.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 12of93
Sequoyah Nuclear Plant Biomonitoring July 25 -August 01, 2017 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser pischarge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 13of93
. Table B-L Sequoyah Nuclear Plant Outfall IOI Diffuser Discharge Concen,t~atioris bf Chemicals Used to Control Microbiologically
- Induced Corrosion and. Mq~lusks, During Toxicity Test Sampling, Februafy 6; 2005 -July 28i 2017 Date .. 'Sbdiutn* . Towerbrom PCL-222:
- PCL-401 CJ;.,-363 Cuprostat-PF H.::BoM Nalco 'H:,150M Hypochlo~ie lngJL .* iiiWt: **..
- mgJL mgJL mgJL mWL . 73551 mg/L *.
* ..:.'.;,~*-~: TRC ':i>ijosphate'~ Copolymer <nM:AD> Azole Quat mg/L . :QWit: .
EO/PO 02/06/2005 <0.0042 ::.... 0:028*~~ 0.010 :, -..:*: 02/07/2005 <0.0116 : 0.028**
- 0.010 0.007. r' -*-- '
02/08/2005 <0.0080 1<
- 0:028 <* 0.010 02/09/2005 0.0199 " 0*:048:. 0.010 *-
02/10/2005 <0.0042 0.028 ,.
., Q,028._.
o:cno *- - 0.007 02/1112005 0.0155 0.010 '.** 06/05/2005 0.0063 .~;*-: */ *- 06/06/2005 '0.0043 ,0.037-. 06/07/2005 0.0103 0.037* . 06/08/2005 0.0295 .. *.* . o.p~7 06/09/2005 0.0129 06/10i2005 0.0184 07117/2005 0.0109 0:026 0.009 07/18/2005 0.0150 :- 0;1)26.; 0.009 Q.036 07119/2005 0.0163 : Q,026 0.009 0.036 07/20/2005 0.0209 '0.02.6 0.009 . -
'. ' 0.014 0.036 07/2112005 0.0242 :0:.026'" 0.009 07/22/2005 *- 0.0238 . 0:0.?4~. ;> 0.018 0.014 ', .- . "
10/30/2005 0.0068 10/31/2005 0.0112 -. .. 1110112005 0.0104 *- .- *0.035. -* 11/02/2005 0.0104
- 0:036."<
. , ~
0;036 .* 11/03/2005 0.0117 11104/2005 ',* .J' 0.0165 _o,.m5*. 11114/2005 0.0274 11/15/2005 0.0256 11116/2005 0.0234 *' . 11/17/2005 .' 0.0231
,*':*:r*
11/18/2005 0.0200 11119/2005 " . . . - 0.0116 Page 14 of93
Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and 'Mdllusks, During Toxicity*Test Sampling,
- February 6, 2005 -July 28, 2017 Date Sodium Towerbrorn PCL-222
- PCL-401 CL-363 Cuprostat-PF H"BOM Nalco H-150M* MSW Hyll_ochl?rite mg/L mgtL-*. mg/L mg/L *'. mg/L mg/L.: 73551' nig/L 101
*. ,mg!L. TRC Phosphate Copolymer DMAD. Azole Quat mg/L Quilt mg/L
. TRC EO/PO. Phosphate 11112/2006 0.0055 11113/2006 0.0068 *.* *- ' .*';
- 0,037, ;
11114/2006 0.0143 -- '. . 0.037*. 11115/2006 0.0068 .* 0.037
- 1,'
11116/2006 0.0267 0.037 11/17/2006 0.0222 -, .' ' . - j 11126/2006 0.0188 ,. 11127/2006 0.0138 -*~*. \ ., ' 11/28/2006 0.0120 11/29/2006 0.0288 . I. 11130/2006 0.0376 12/0112006 0.0187 ..., 05/28/07 0.015 05/29/07 *o:'o'36*
- 0.015 05/30/07 0.0084 0.017 0.036 0.015 05/31107 .;, , "\
0.0103 . '0.036 0.015 06/01107 0.0164 0.017 0.036 0.015 06/02/07 0.0305 I .* *- O.Q15 12/02/07 0.0241 12/03/07 0.0128 I* 12/04/07 0.0238 t* 12/05/07 0.0158 ' - 12/06/07 0.0162 12/07/07 "' ,1, 0.0175 ;. 04/13/08 0.0039 '"' /;' ... ~* 04/14/08 0.0124 04/15/08 . 0.0229 04/16/08 0.0143 04/17/08 0.0120 ~-**.~:
"£*
'.1 A ["
' f 04/18/08 0.0149 10/26/08 0.0260
- 10/27/08 0.0151 0.017 10/28/08 0.0172 ..., '*** ' 0.041 10/29/08 0.0154 0.018 0.0,~l : 0.030 10/30/08 le : 0.941 0.030 10/31108 0~0086 ~' 0.0~.1 .. 0.030 Page 15 of93
.. . . Table B-1. Sequoyah Nuclear Plant Outfall IOl
- Diffuser Discharge C~ncerttrations of Chemicals Used to Control MicrobiologicaHy Induced Corrosion and Mollusks, .During Toxicity Test Sampling, *
- *February 6, 2005 -July 28, 2017 .
Date , Sodiu~ 1 . Towerbrom .P.CL-. PCL-401 ~~~36_3., Cuprostat H-1$0.fy.1 *Nalco .Specfills.
- H-150M . M~W.
Hypochlonte mg/L ' i2i~~ mg/L . riJ.glL' < -PF mg/L
- ing/lf *. 73551 .
- CJ:l300 *. mg/L !: ':JOI: .
*
- mg/L* *
- TRC mg!C'. Copolymer DMAD Azole '*Quat'.: mg/L mgJL**. Quat 'irig!L. *'
.TRC~: Phospll:* EOIPO " Qilaf .: Phosphate ate_,.* *. '*"
02/08/09 .. ~ ~- 0.0197 , - '" 0.017 02109109 - . 0.0237
.. . 0.017 02/10/09. 0 ..0104
- 0.021 02/11/09 0.0155 - .
0.017 :~ .. 02/12/09 0.0106 0.017 02/13/09 ,*.,.
~
'H*
- ,.'*I.
05/10/09 ,~. 0.0129 05/11/09 0.0415 -*. 0.0446 05/12/09 .. , *. 0.0053 ..
, . *' "~. ' '
0.0396 05/13/09 ' - 0.0049
' ~
-*. 0.0396.
05114109 I
.: <o'.0141 1'
0.0397 05/15/09 <0.0160 - 11/15/09 0.025 -* *-
- .. ,c.
11/16/09 ' - 0.0152 - 11/17/09 ~ 0.0255 *- -* - ,' 11/18/09 - - ~: '; 0.0306 ~ 11/19/09 - , 0.0204 '* 11/20/09 0.0093 ~ 05/09/10 0.0192 05/10/10 -
,.*, 0.0055 .. ~~
' *- "/. 'J ,',q_
o-.<i39 ~,: 05/11/10 - 0.0100 :_. 05/12/10 - ._.,. 0.0171 ,,., . 0.039 05/13/10 0.0041 0.039 05/14/10 0.0099 ,'"'
~ :
0.039 Page 16 of 93
- Table B-1. Sequoyah Nuclear Plant Outfall 101
. Diffuser Discharge Concentratfotis 1of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Te~t Sampling, February 6, 2005 - JUiy 28, 2017 Date :Sodium Towerbro PCL-401 .3qt::3.6?. Cuprostat ff:J,3~]\.1 Nalco. Sp~ctrlis; H-150M *MSW Floguard
*Hypo- mg/L ~22: mg/L ** *~g/B,, -PF mg/L .;tngl,L,~,; 73551 *CT1300
- mg/L . ?J.0,(.*. MS6236 chl&i-ite. TRC . rilg/L.:: Copoly- p,~:, Azole ** iQuaf:~: mg/L Quat *
- illg;L mg/L
*mgffi Phos.:.. mer .. .. EO/PO ;~~ps+; Phosphate
- me': ** hate.* *:. hate 10/31/10 11/01/10 11/02/10 11/03/10 11/04/10 11/05/10 05/01/2011 05/02/2011 05/03/2011 .
05/04/2011 . 05/05/2011 05/06/2011 11/06/2011 11/07/2011 11/08/2011 11/09/2011 11/10/2011 . 11/11/2011 : 05/06/2012 05/07/2012 05/08/2012 05/09/2012 . 05/10/2012 05/11/2012 08/12/2012 0.029 08/13/2012 0.029 08/14/2012 0.029 08/15/2012 0.029 08/16/2012 0.029 08/17/2012 0.032 05/12/2013 05/13/2013 0.064 05/14/2013 0.06'4 05/15/2013 O.OM 05/16/2013 0.032 05/17/2013 0.032 09/15/2013 0.03 09/16/2013 0.03 09/17/2013 . 0.03 09/18/2013 0.03 09/19/2013 0.03 09/20/2013 . 0.03 Page 17 of93
~-------- ----- - -
Table B-1. Sequoyah Nuclear Plant Outfall IOI of
/Diffuser Discharge Cbncentrations Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling,
* . February 6, .2005- . July 28, 2017. .*
Date S,odium Towerbro *'.<;~~3.p3'..; Cuprostat JEt3.0M M~W: Floguard
- ayp~;: mg/L '* nigi'E:*,< -PF mg/L t,1mg!L *.. .ror.: Ms6236 chlorite~ TRC . . r>~.. . Azole ,.)~u~f,/ . 'tng/1,2 '. mg/L
*'fu,g/L* ,~~ I ' ' '" r
.. 'i>ijo~.:: Phosphate me hate~
05/04/2014 0.0118 05/05/2014 0.0112 05/06/2014 0.0096 05/07/2014 0.0164 05/08/2014 0.0235 05/09/2014 0.0110 09/07/2014 09/08/2014 09/09/2014 09/10/2014 ' 09/11/2014 0.0070 09/12/2014 0.0074 08/09/2015 08/10/2015 *. 08/11/2015 . 08/12/2015 . 0.03 08/13/2015 0.03 08/14/2015 0.03 10/18/2015 10/19/2015 10/20/2015 10/21/2015 . 10/22/2015 .*. 10/23/2015 05/15/2016 05/16/2016 05/17/2016 05/18/2016 05/19/2016 05/20/2016 07/31/2016 08/01/2016 08/02/2016 08/03/2016 08/04/2016 08/05/2016 04/30/2017, 05/01/2017 05/02/2017 05/03/2017 ' 05/04/2017 05/05/2017 Page 18 of93
Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations bf Chemicals Used. to Control Microbiologic'ally Induced Corrosion and MolluskS, During Toxidty Test Sampling, February 6, 2005 -July 28, 2017 i Date Towerbrom* Floguard Spectriis BD 1500 Nalco Spectrus Floguard mg/L MS 6237 mg/L 73551 . CT1300 : MS6236 TRC mg/L * .Quat mg/L :., nig/L ' mg/L
" . Phosphate/Zinc EO/PO .
Quat ..i ** -Phosphate 07/23/2017 - " - ', 07/24/2017 0.0124 0.04/0.01 - ., 07/25/2017 0.0081 . , 0.04/0.01 - ' 1 07/26/2017 *' 0.0232 0.04/0:01 .. ':0~0'3 ." :, ' 07/27/2017 0.0179 - - . 07/28/2017 " 0.0296., - 0.03~ Page 19 of93
Sequoyah Nuclear Plant Biomonitoring July 25-August 01, 2017 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 20 of93
BIOMONITORJNG CHAIN OF CUSTODY RECORD Page _1_ of_l_
~ent: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):*. ~ject Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus* Client ~*Number: NIA Asheville, NC Other (specify): :Son; Q.. Pe.\;\/~ I y
- nciiity Sampled: SeqLloyah NP 28801 NP DES Nu111ber: TN0026450 Phone: 828-3 50-93'64 Fax: 828~350-9368 Field !dcnfffication I 0'mb/Comp "" Collection Datemmc. Container Flow Sumplc Description Number 8{, (MGI?) Rain Event?
Volume (Mark as Appropriate) Collected Yes Tf¥es, No Tr;1ce P.T~n.og Ariivill 'fe111p. Oy Time Appear* Inches Nuniber ("C) ancc, Start End 'E..-r
~-23*17 SQN-101-TOX Comp o7So ~
I (2.5gal) o.J1 \~1J11'f.b I :'2 ,\'C O' '""'"!f:> f.. SQN-INT-TOX Comp 7*7.:J.-/7 oY/6 ~ 7 .. Jl/47 , l (2.5 gal). p? /.!> ff..i:'
. NA 'x ....3_7/)*
l'to;ci~.02... ().IQ"(_ 't'- ', ,~<., ~ Sample Custody - Fili ln From Top Down Jf.. c..u~'"t'()"b,"-\ Se.t>..~ '~~(.,'~ $p...\-\1"..5 *~1,JeJ) Ul~~\\10N. \RLA~':.~~ I..., Pl.LL Relinquished By (Signature): Date/Time Received By (Signature): Date/Time 'ii At,\:'1~ .
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Instructions: Cli\~nt!l should fill ill all nrcns except those in the .. l.ahoratory lJge'" bli:1ck. Biomonitoring samples 11rc prese!'Vcd by stod11g them at 6C and shipping them in ice. The hnld ti11 1c for each sampli: i:; 36 hours frnm the ti111c of collection. Therefore, plcnsc collect and ship ir'< such n wny that the luboratory will receive rhc smnplcs \\'ith ample linw to initii1le testing within that rime* frame. Samples shipped overnlghl on Fl'ida}; via FedEx or*UPS r'rlusl he mnrked lor Saturday delivery tir lhcy w'ill m1l arrive until the lbllowing Munday.
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Whole Effluent Sample Receipt Log Page~
'Sample cemper~ture performed usine Sample Rec_.;IYln~ Thermometer: SN 160928522 Date Time Received Received "Sample Project Sample 0
Sample name and description State Comments Recehieu Received by 'irom 1emp. \ .q nt1mher number 07;24.17 1235 J. Sumner TVA Courier :1..1 12585 170724 .01 TVA-Sequoyah Nuclear.Plant-101 TN. 07-2{1*17 1235 t Sumner. TVA.Courier 0.6 12585 17072.4 .02. TVA* Sequoyah Nuclear Plant- Intake TN SOP G4 -1!.-<hlblt <;4.2, revision 01-03-J2
BIOMON1TORING CHAIN OF CUSTODY RECORD Page._1_ of l
~en!: TVA Environmental Te~ting Solution;, Inc. :Delivered By (Circle One): ~ ~ject Name: Sequoyah NP ToxiCity 351 Depot Street. FedEx UPS Bus Clieni ~*Numb.en N/A Asheville, NC Other (specify): SDn,~(I Ve\ i' vef,t/ ~
r:acility Sampled: Sequoyah NP 28801 Generaf'Comme~: @ .715 7¢/C ~j>IV:t -t'd,.., l~I ,j.,.:;f~ c; _ NPDES Number: TN002645Q Collected By: Mar~ o. Maorc. bc-)"flor.
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Phone: Fax: 828-350-9364 828-3~0--9368
/;cl! Sa. ...plt!J ./("()m .:M /. e ~7/ey,£ 1)77/0
* ~\)iss. Me.to!~ ~ue CoUe("#+ed ¥ 1'..~4 r
D'rl '61-k Field lde0titica1i_on I Grob/Comp Collection Datcffime Container Flow Rain Event? p!Wjuf~ (258S Sample Descriprion Nuinbcr & (MGi:)) Laboratory Use. Volume (Mark as Appropriate) Collected ---
- . - Yes lfVes, No* Trace ETS Log Atiival Temp, 8}' lm1c J\ppear-
-- End
-- Inches Number ("C) nnce Start E.."t'"
x SQN-IOl~TOX Comp 7-JS:l'-1 ?'-~*1'7 1 (2.Sgal) 1J'1Zo ~r *ohi.o rr - J<//~.~n \1t>1-'2.ho.0\
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\nstrnctions: Cllen\s*shmi\d fill in \\ll arca;.exc.:c:pl those in lhc "Laboratory Use" block, Biomonitoring samplr!s are preserved by storing them al 6"C 1i.nd shipping lhcm in ice. The hold time for each sample is 3(i liour~ from the tiinc of collection. Therefore. plc:1sc collect and ship in such a way that the laboratory will receive the i;amplcs with <lmplc time to initiate icsting i.vithin that time frnme. Satuples shipped twcrnighl on Friday via FedF.'.x or UPS 111u:;t be m3rkcd for Saturday delivery or they will t\01 arrive unril the following Monday. -
Whole. Effluent.Sample Receipt Log i>age qz_,
*sample temperature pcrrorrne~ using Sample fiecelirlng illennometer: SN 160928622 Date TI me Received Received *Sample f'roject
- Sample Sample name and description State Comments Received Recefved by from Temp. (°C) number numb.er 07-26,17 1232 J. Sumner TVA Courier 2.6 12585 ' 170726 .01 WA - Sequoyah Nuclear Plant- :101 TN 07-26.-17 1232' J. Sumner TVA Courier '2.7 12585 170.72.6 :02 TYA- Sequoyah Nuclear Plant*. intake.. TN SOP Gtl - Exhibit Gll.2, revision OJ,-03--li
BIOMONITORING CHAIN OF CUSTODY RECORD Page_l __ of 1 C"i)ent' TV A Environmental Testing Solution, Inc. Dellvered By (Girc\e One):
-~
Jt&,ject Name: Sequoyah NP Toxicity 351 Depot S tteet. FedEx BPS Bus Client
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P~. Number: N/ A Asheville, NC Other (specify); .:Sn(\\.<!.. De-\i vef.V
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<o . General. Comments* . ~
2880l llatility Sampled: Sequoyah NP TooK Sa;~~b f'rc...., \ti\ ~a..w;~l'er@._ lJ7.5(..) r-6" . - Phone: 828-350-9364 tot>~ f::r;....,... _le.' .ft,o ~ ~e... ~~l-er@. v 7.._...,-.::;, NPDES Number: TN0026450 Colloored B~? ;,::;;x,
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Fai: 828-350-9368
- tO<s.s. i A.,,J.cJs ,..:o,.:.r*c tJr.11~ du/ + W4 ~" ~ik l ield tdcntificatloo / Flow pn,oj v:t ll t~*
1 Grab/Comp Collcctioli Dactffime Container Sample Description - Number& (MGO) Rain Event? Laboratory Use Volnmc (MarK as Appropriate) Collected
--- - Yes If Yes, No . Trace ETS Lng Arrival Temp: By 'Time Appear-
-- -*- - Inches Numb1:_r (°C) an cc
.Start End ~
1-zg.;7 r;-;z'l-17 x \:ro~28.Dl \.:'\ \.'\*it ~ SQN-1()1-TOX 2 (2.5gal) Comp
. tJ.73~ !1 /J&J6 1;7' 11?1.11 l). 9'-/ \'\"':>-\
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- 7,zF--17 SQN-INTcTQX Comp '/'Z'/*1'7
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. Sample Custody-' Fill Tn Frpm Top Down
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lns1ructinns;* Clients should fill in all area~ cxc.:pt 1ho:;e in the l.nbornlory lise" block. B101110nitoring ~amples nrc prcscrvctl by storing then1 al. 6"C and shipping them in ict:. The hold time rt1r <:adi ~ample is 36 hours lh1m the time (If c0\tccll<1n. Tl1crc.fl\n!. plea~~ collctl m'1d ,;hip in sud\ 11 w11y Iha\ lhc lahornlmy will receive \he snmplc;; with ample 1i1nc lo iniliatc le.sling wilhi111h:11 ti1ne thune. Smtipks shipped overnight on Friday via FedEx or UPS must be market! for gaturday delivery or they will not arrive until the following Monday,
Whole Effluent Sample Receipt Log
'Sample temperntu~ performed using Sample l\eceMnr. Thermometer: SN 160928622 Date 1irile Receive*d Received "'"Sample Project Sample 0
Sample name and description State Comments Rect;ived R_eceived by from* Temp *. ( C) number number 07~28-17 1234 J, Sumner TVA Courier. 1.7/1.9* 12585 170728 .OJ TVA- Sequoyah Nuclear Plant* 101 TN 07.-28;17' 1234 J.Sumner. TVA Courier 0.9 125SS 170728 .02 TVA* Sequoyah Nucle?.r Plant- Intake* . TN SOP G11 - Exhibit G4.2, revision 01-cl3~n
Page I l1f7 Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013 Method 1000.0) Speci¢s: Pimeplwles .promelas CHent: Tennessee Vallev Authorit;* County: Hamilton Facility: Sequoyah Nuclear Plant Outfall: 101 NPDES #: TN0026450 Pro,iect#: * \""l..SRS
.Dil11tio11 vrepamtio11 information: Comments:.
Dilution prep(%) 10.7 2L4 42.S* 85.6* IOO Each concentration was UV-treated Effiucnt volume (rnL) 267;5 535 1070 21'10 2500 for2 minutes.to remove pathogenic*
*Diluent voluinc: (niLJ 2232.5 1965 1430 360 0 . Interferences.
Totalvolmnc (ml) 2500 2500 2500 2500 2500 Test organis111,i11formatio11: 1'esr i11formaiio11: Organ)sm source: In-house culture . Randomizing template: UErtlr OMN~ Age: < 24-llours old lncubator number and shelf location: 1 e., Spawn date: O"l*\C\* n Arremia CI*fM numbitr: CHM914 Hatch dates,and times: Oi*-1.'-\* t'"l \'oC::.O ~ JJryi11g iliformaliollfor weight
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- n 6\o~ delermi11a1io11:
Transfer vesi;elinfonnation: pH= ., .'°\'"\ s,u_ Date I Time in.oven: ofMt~-s"\ rJeic; Temperature*= "'U\*'.1;.. oc IIiitial oven temperature: lah' (
* . Average transfer volume: <0.25 mL Date I Time out of oven: 1'.i-01.*ti OSie Final oven temperature:: \tit!.(.,,
Total drying time: '1..'.\.~ llb.J.Q s. Dail)'feedilzg a11d re11ew11l i11Jormntio11: Dn_y Date Morning feeding Afternoon feeding. Tcsl hJitintiun,. Snmplc numbers used i\'11-JSW
. renewal, or termination batch used Time Analyst* Time Analyst Time Annlyst Outfnll 101 lntnkc 0
.. 07-25-17 .()t.~S. ~\ \..,_J.o ~{ .M~?f:. \1 '1.<!'11.'-\.o \ nm.."£:\. o1- Ul*lS*J1.
I 07:..26~11 oroos \( n.'40. J( 0 lO<i \.1. l'""\ 0'\1.'-l-ti \ l\C'11.'-\ *01. tr\-\'i* \1 v 2 07~27~17 Olci~~ H
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v I' Acccplnnce cri,tcria Conlwl infonuatii:m: *\)'1-'Ta:er-.~~b Summary oftest eniluoints:
* % Mortality: 61. s20% 7-day LCso > (()\)'1_
Average weight pe" initial larvae: o.9ot\ ' NOEC lOC1. Average weight per surviving larvae: <:i .s~~ > 0.25mgllarvnc LOEC 'IC\f\1 ChV '";) rnc1.
*IC2s >te-07.
SOP AT20*-Exhibit AT20.3, revision 11-01-14 Page 27 of93
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Page2 of7 I Species: Pimepluiles promelas Client: TVAI.Sequoyah Nuclear-Pfo11t Outfall 101. UV~treated Date: 07-25-17 Survival mul Growth Data Day CONTROL 10,73 21A%. A B c D E
.F G H *1 J K L 0
lO ID .JO J'O JO JO 10 10 ID ro JO JO l JO 10 lo (0 lo {C) to 10 10 ID 10 10 2 I() lO IC l \'.) to lo: lO 10 to lQ. fD to 3 10 to io l l) tD f.0 LO to lO co IO 10 4 ID 10 10. l C>> 10 IQ to 10 l () IQ co (b 5 to 10 lb ID ID ro ro IQ IO lO 10 10 6 I t.i lO 10 .. co /,0 IO 10 tO IQ LO ro £Q 7 t () 10 10 IO lO I\) JO LO IO 10 ID IQ
. A =Pan weight (mg)
- Trnycolarcode::, ~
Analyst: 'ldtYv1
- Date: QJ1~!:\-l"l
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8 = 1":111 + Ln~c wc!gbt.(mg) Analyst: * "'Z,\."A "l.\.lo"\ '2.ti.CQ 1.\.aC\ '1.\."\li '"lo"l*it\ '2.\,'\!> '2\.~ -z\~~o -u.~ C.'1.o5 "'tt.~ Onte: aa*<r'::i*\~ C= Larvae i1*cigbt(mg}"' B-A
. !land c;1lculated. ~
s.&~ 'S .0:.1.a s,:1.~ b,o"L C...\~ ~.u.g ~;as !,.So I;..~'!\ -;,?.&.\ c.o.~ s ,"ile. A11:1lyst: . Weigh I 11cr in Hin I number of lnr.vnc (mg)
= C / lnilinl uumbcr oflnrvnc ~
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Avcr:1ge weight per rcr.ccnl rctluclion. i' ~ *. ' J 0; "1 '1..'-\ ().loo:!> 7. initin! number of from control(%) 0 . sto'\ "" . , ..:.* j - '1.'17. -lo.\ lnrvuc (mg) . !:--~~-----* .:. -~~* ._,. Comment codes: .c =clear, d = dead, fg = fungus, k = ki lle9, m = missing, sk =sick, sm = unusually small, lg= unusually large, d&r= decanted and retu1i1ed, w = w!lunded. Co111me11ts: Page 28 of93 SOP AT20- Exhibit AT20.J, revision l 1-0J-:J4
Page 3 c*f7 l l Spceies: Pimephnles promelns Client: TVA/ Sequoyah NuclearPJ:mt. Outfall 101. UV-treated Date: 07-25-17 S1irvival amt Growth D11ta Day 42.8% 85.6% JOO% M N 0 p Q R s T u v w x O* JD JO JO JO JO JO lO 10 10 10 JO IO l (0 . lO lO ~o lQ !O l ti IQ lO IQ IO 10 2 I0 *10 10 IG to lO to fO I{) <o to ro 3
\0 10 /0 t*o LO [0 It) (CJ IQ tO ID '0 4
ID ' t> IC lb to 10" l t> I~ c () \ C> t (J i () 5 l l) ( (} .to l tJ l() lO <o /0 l{J ( tl IQ l(J 6 lQ '{) lQ ' l () rt> rD l fi IG c{J 10 I\) ft) 7 I \J lD 'lo. I () /Q ff:) I~ , ti I ti I() I \J 'D A= Pnn wciglil (mg) Trny color tollc::: ~_r,k
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- B =Pan -r L:i1\ might (ing)
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t>* '*"' ~ Annlyst; Date: o&-~*\1
"?.\.i~ ~\.~~ '11.*SC\ ,.~ \~*. -wf\~ "'l,.\.Si i.\.1:1'-\ I.OJ.'\ -uf\l.o "2.~.'\1 -1.1. 1.\i l't.'$.(
l C= Lnrvacwcighl (mgf= ll-A Hnml c:ilculntctl. t.*'°"' t..ott te..S4\ (o_'5'i. '$.11.:i L>.C\G to.l.\o *S.'lo~ <.o, \"\ s.10 S.lot s .'-!Cl f\n:ilyst: fi\. ' Weight per inilinl numllcr oflnrvnc (mg)
*= C / lnitinl 1111n1bcr.ol' lnn*nc
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c>> e>' o* o* t:i* o* -j Annlyst: _ .<<;)" r:l Avcr:igc weight per , Pcrccmt reduction
.initi:11 *nunibe1* of
_.Jarvac (mi?)
- from <:onlrol (%) o.t,'-\"1 -t-=- a7. (). r;'\8 -~.\ 1. D, 51':-\ - 0. q 7.
Comment codes:* c*= clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm = unusually small, lg= unusually* large, d&r = decanted and returned, w = wounded. _J Comme1zts: I Page 29 of93 SOP AT20 - Exhibit AT20.3, revision I 1-01-14
.,1-j ~:~YF Page 4 of7 Species: Pimepltales promelas Client: TVA I Seguovah Nuclear Plant. Outfall 101*. UV-trcated Date: 07-25-17 Survival am/Gl'owtfr Data Day 100% Intake Control - Non-treated Y Z ' AA_ BB CC DD EE FF ..GG: Jj_H
- b J..J 0
10 10 10. IO 10 IO 10 10 1 10 I0 \ t> co l0 /Q (0 10 10 J0 10 co fe> I0 lQ .10 3 t.0 IO fQ IQ f 0 IO tO . ID Io 'f 0 to to ca 10 tl) ro 5 II,) LO 6 10 10 Jo to JQ IQ IQ 10 7 I0 l0 lO ['{) lQ A= ran wcighf(mg) Tray color code:: '{}'\~+/-o....
.\n~lyst: 1¥'rYi Date: 07- 2-4-(1
-B =Pan + Lafr.rvc weight (mg)
Anulyst: --~'-------- Date: *qb*\-"\
- s. ~o s.'\1. s.-s1 -s.S'S Hand calcul:ttctl.A '~~i0-~ ~ { - _ :: t..r..o r..ns. s.i.,,ti 1o.-:ib Amtlyst: ---i(;}.!,.i------ c-*-- o ... ,, Q Weight per initial number orrarvn<: (mg)
=CI lnilinl 1111111bcr orl:in*ac llnnd cnlcnlntcd. yl A1inlyst; ----~~-----
.,\\*crngc weigh! per Percent reduction initial number of from control'(%) \_;. &7.
larv:1c (mg) Comment codes: c =clear, d ""dead, fg = fungus, k =killed, rn = missing, sk =sick, sm =unusually small, lg= unusually large~ d&r = decanted and returned, w = wounded. Comments: Page 30 of93 SOP AT20 - ExhibitAT20.3, revision 11-01-14
TV A I Scquoyi1h Nuclear Pinnt, Outfall 1(11 July 25- August 01, 2017 N111epl1i1/i!.~ pro111ii11~* Ciih111if..-. Wholc*l!:rnuon*r To~icity Test-l~PA*821-R-02-0IJ~ l\*lctl1ml IOOO,o Q1111llly Contl'Oi Vi!rlllcnliim 01' oaia i~nfry, c111cuht1io11s, nnll Slalisili:nl.i\nnlym
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--11 *io IO - IHI 21.~7 6.06 0.606. ..: - .0.606 85,6~~ o~4s ~-~ 11111.u o.s?s .;,.! ;.5"~1 s ; JO 10' 14.V.I. '21.)4 6,JQ U.6-10; '0,6-10 *.
'I' *lt)- 10. U*lU'. l0.89 S.6?* o.;69* ....... 0.ML ..
11* *to_ IQ 14.19' 20.Q6._ u.17 0 617*" 0.617' v* 10 . 10 15.~2- 20.9! s:;o [*Siil .Q..170
!011% .ll.:\7-1 Sb 100.0 0.574 ~.G -ll,9 w *10* *w 15.49 ~!.16' *s.68
- O.S6S '0$(18 x JO: . JO l~.l(l 19.50 5 JO *O*jJO' 0.5*IO y .10. 'IO l~.67 19.97' 5:.lO* O:$JO 0530
.'l* .Ill.. -10. 16.26 21',18 59? R$9l O.!i'J1 IOD% lnt:.tke 11*5~'! -16 ,IOM *U.SM ~.ii 1.8
.AA *W 10" 16.IJ 21.70 *s.s1 0.5~7. .0.~57
*uo 10 10 lS.62 21.17.' sss. - .O.$S.~* -11555 01t1r.ll l!ll: MSI)~ Minimum Sl111iiftc:i11' Dilt~ri:n,c
. ')uUt1fti'J.:\1.SO \t*llttt: O.o"i i*~l~tl. l~f(\.'ill ~lml1ll1Jlll S1~11ifl11<1.id DilTi.TI:m:.; l*MSO: . 9.-l I't\f~IJ i~ a "'~'m:*OrtcS1 pti..~-ision "lf\i) l\\1Stl '111o U\\) t1111\lf1\i11~1 p;:-1cl.l'ul .,b0~1.:uc~ l!i:tw(!.:n llii.: CQ11irut nml m::im1 ..111 l_l1:u c~n bl) di:ik!Jtl!d !lt~UMic:.i!I)' ~*~nUh*~111t lo i\ \~{\~)J~ ¢"i\ll!l!ll\ ')"~il!Uy.t~~' lirf11lw: Uum1ciir 111 ;\1Sb \"11u~: o.ll~ll6 1.o",.-., l'MSll l1<iHool dol<IJO\in<IJ by USEP1\ (lU1h 11<t<*'nlllo} ".I:!~> l'M!I01 7.1 IJpp<1 l'MSl1 bo1mJ d*l<<m~icd lw 11sr:1*,1 (~IIl*h ,,.1w;Ufo) ' 1*l'l~..
TVA /Seq11oyah Nuclear Pfant,.Ou.tt'all lOl Jul11 ?5;;.August01, 2fU7
.: .,_~~ i~~u.:'-J /:-;-: : :;'
Stntis1icnf. *A:n~lysc:s
*Larval Flsh*Growtlt and Survl11:d*Test-7 Day Growth
/Start ~te: 712512017 Test ID: PpFRCR Sample ID: TV!'\ I SON 1Q1 End Date: ai112011 Lab ID: Eis:Enllir: Test.ing *~oi. :;i~mP.re Type<. *0MR-01~cilarge Moliiionng Report Sampfe"Dale<; July ..2017 Prol<Ji:ot FWCHR-EPA'.821 *R-62-013 Test.Species: pp;PimC1phales promelas Comments~ :UV-Treated*
*cone-%* 1 ~ 3 4
-- uv.con1ra1 -- *o.ssao *o.ssso '0:5280 '1.6020 Noo-<::onlrnl *o,6600 *Q,6050 '0.5600 . *0.6360 10;7 016120* '0,6480 '0,5850 *o:s5cio 21.4 0,6490* 0 .5840 0.6030 *o;s*r!lo 42:8. 0.6640 0.6090 0.6590 '0}~550 ai;.s. o.57so 0.6060 0.6400 *o:ssso 100 0.61i0 0.5700 0.5680 0:5400 intake o.~oo o.5926 .0;:3570 0;5550 .
. Transform: Unlr.insformod. 1-Tallcd .Isotonic cone-% Mean. N*Mean *Mean Min: .Mar CV% .. .N t-Stat . *Critical ..llJlSD. .Maari N,Miian*
uv.Gonlro!' iO!i685 0.9240 .0.5685. Q.52BU 0.6020* 5,533, .i* *,0;610.5 1,0QOO (\ICO*COnlrol Q,6153 t:OOIJO 0.6153 0.5600 o.s*sao. 7.017' ii 10,1 .0.6238 1;0133 D.6236 0;5850 o. 6500 ~.999 4. ,2,.501 24ttl' Q,0532 ti:6tQ5 t:aoca zr;~. *0.6030 0;$601 .ti.6oso O;!i76ci o,549p* -5.42.1 ~-. -1,562 *2'.410. i>;ass2* 0.6105 ,;oooo 42.8 ci..j465 1.0512. 0.6468 o;so9p 0.6641? a,933* 3;54~ 2.4.10 0.0532 06105 i:oooir
.85,6 0.5978 0.9i'IG . 0.5976 0:5690 0:6400 61423 "
~ ,1.:.124 2.'111) o:os~2 o..s!,r7a. \),9791 100 o.$i3a **0,9325 0;5738 o.5460 o,imo :5;563 :?;410 o.osn Oo/'.38 0,B39B lntai-:e 0.5585 o,9076 6.5535 05300 0.?920 4;564 "
4
*0. 238 Auxiliary Tasts .Stalislic; .Critical Skow. .Kurt*
Shapiro-Wilk'slest i11tlieates normal dlstnbuUon (p> 0:01) 0:94732 . Q.684 iO. t2992 -1.1710 Bartlett's TesHndica!es equal ilai'lani:eii IP-= 1.00) 0,.2Ji6' .15.086J.
lhe contn:il'ffi.,ans are'nol*siQniilcanllv dlfloront
100 o.05324 o;on:isa 1).00353 '0.000913 '0:01933* s;1a
- ricatments vs: w;conlrol .. *-- . . ..
. Linear lnlorpolatlon 120q RcsarnplDs) S5% CL:(EKP) Skow . . IC05 96.265 IC to >106 IC15 >mo Ul-.--------------. *IC20* >100 [!.9 IC25 >1D(I lia* JC40 :,JOO IC50 >100. Oi? 0.6 *~g:, (/.5 O:*l
- g,,1)3 ir:
**0,,2 ll.1
- 0.11 Q>-<>--o:-~-__..r
-0;1 *** * * ..... **!' *0,2 +..-....,..-..,-.-~~~-.--.-"'""..;.;..-i 0 so 100 ..... * ...... ~ .... *....... - .... - ......................... - * * * * ~ ... ~ "" ,., *'I, 1.1a11, o.os revel 0.5 01 signlfieaiico * *~ OA '3' - ~*a:J* 0 .. a~ or 0 e* =i z Page 32 of93 TVA/ Sequoyah Nuclea1* Plant, Outfall 101 - .Intake July 25 - August 01, 2017 Statistical Analyses ' Envlronmunt.i' T11s1lngSolul10n"..._f111;.:. Larval Fish Growth and Sur.*ival_Test-70ay Growth Start Date: 7/25/20'1"/ Test ID: PpFRCR Sample ID: TVA I SQN 101 - Intake End Date; 8/1/20'17 Lab ID: ETS-Envir. Tesling Sol. Sample iype*. DMR*Discharge Monitoiing Report Sample Date: ,luly 2017 Protocol: FWCHR-EPA-821-R-02-013 Tes1 Species: l?P*Pih1ephales promeias Comments: uv~Treated Cone-% 1 2 3 4. UV-Control 0.5880 0.5560 o:s2ao* 0;6020 Non-control o.6eoo 0.6050 0.5600 0.6360 10,7 0.6120 0.6~60 0.5850 0.6500 *21.4 0.6490 0.5840 0.6030 0.5760 42.8 06640 0.6090 0.6590 .0.6550 BS,6 0.5760 0.6060 0.6400 .0.5690 100 0.6170 0.5700 0.5680 0.5400 llltak.e IJ.5300 0.5920, 0.5570 Q.5550 Transform: Untransformed 1-Tililed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD UV*Contfol 0,5685 0.9240 0.5685 0.5280 0.6020 5:833 4 Non-control 0,6153* 1.0000 0.6153 0.5600 0.6600 7.017 4 10.7 Q,6238 1.0138 0.6238 0.5850 0.6500 4.999 4 21.4 0,6Q30 0.9801 0.6030 0.5760 0.6490 5.421 4 42,a 0.646,8 1.0512 0.6468. 0.6090 0,6640 3.933 4 85,6 0:5978 0:9716 0.5978 b.5690 0.6400' 5.423 4 100 *u,5738 0.9325 o.5738 0.5400 O.<lHO '5.563 .i In lake 0.5585 0.9078 0.5565 0.5300 0.5920 4.564 4 0.473 *1:94'3 0.0406 Auxiliary Tests Statistic Critical *Skew Kurt 'Shapiro-Wilk's Test indicates normal distribution (p > 0:01} . 0:93613 0.749 -0:0833 -1.2168 .F-Test indicates equal variances (p ;= O:G8) 1.69266 47.4683 The control means are not significantly different (p 0.14). = '1 .. 71762 2.44691 Hypothesis. Test (1-tail, 0.05) MSDu MSDp MSB' MSE F-Prob df Hornoscedastic t Test indicates no significant differences 0.04064 0.07148 D.0002 0.00087 0.54944 ,.6 Treatments vs UV-Control Dose-Response Plot 1-tail. 0,05 level (Jf significance Page 33 of93 l Page 5 of7 Species: Pimeplutles promelas oatc: 0:t:.2s'"11 Clieut: TV A/ Seqoovah Nuclear Pfaal. Outfall 101. UV-treated -I Dai{~* Clremistf]*: D.a't' (Analvst identified for each *day, pcrforn1cd,11H~ D.O. and, conductivity measurements onlY.i* 0 1 ~ Analyst* HS Concentration Parameter pH (S.U.) DO{mg!L) Conductivity CONTROL (1111ihos/cm) kj\lfrnlfnity* UV-Created *c111g caco,11..J *~Hardness (mg CaCO;/L) "'Temperature (°C) nH (S:U:) DO (1i1g!L). Coudu):tivHy* (1m1hos/cml i'*Tempcrature (0 C) PH (S.U.) ll.4% DO (mg{L) Conductivity ( .. ._:::-**- ;: -,,~ (11mhos/cm) zwz. f;c : ::::, *Tcmperature'(?C) "'\.c;. 0 oH (S.lJ.) D0(mglL) Conduclivity t*~t~~~ - ,- ~ ~f! (iunhos/cm) *!:_.._;:__..:,,:.-;_:;;J . "Temperature ( C) 0 "'\..-<;. 0 CJH (S.ll.) D0fm!1/L) '.to i;:lf,h*--- 85;6% Condncdvity -~ (µmhos/cm) 210 ;_ !!~. -- ' - __'~l *'~Temperature (°C) IS.O pff(S.U.) . B-01 DO (n1g/L) ff.o ConduclMty /~(_*--~~. :. \~', ,>> .LI~~ (fnnhos/cm) . - ~Allcnlinity 100% .(mg CaC03'L) l00% lutake "Temperature <"Cl 1.'i. '2.. 1.Ll. b 1..4:.*1 {...'{.~ ~.Q ic.\*.ln. -Initial Finni Initial . Finni lnitfal Final * . cmpcrnmrcs performed al Chi: tim;:: oflesl initiation, renewal or tcmiinntiqn by the analyst identified in the Daily Renewal lnfarmntion table loClltcct 'Oil Page I. ' ::.:J}JK* inity. hardness and total residual chlorine pcrforn\cd by the nn~lyst ic:fcntificd 011 lhc bench sheet specific for each analysis and lnmscribcd lo this b~nch sheet ,.,,.*L*l\y;,, _ Total residunl chloiim**vas pcrfonncd on.non-trcmcd OutfalHOl :111d lntnl;e samples. .SOPAT20-ExhibitAt20.3, revision I f-OI-14
- p~,.-?*
~r.:m 14 t~'-. Page 6 nf7 l Species: Plmeplwleniromelas . Client: TVA /Se uovah NuClear Plant. Outfall 101. UV-treated Date: 07-25.;.17 l Dav *(Analysl identified fore:acll day, perfom1cd.pH~*D.b. and c.:inductivily n1ensutc111.:nts oril~*,) 3 4 5 6 Analyst I Concen-tration Pnr:imeter DO!m.;L} *Conductivity CONTROL (1m1hos/cml "Alluilinity uv..:tre1.1ted (mg CnCO;/L) ' '.>Hardness {mg CaC0;/L) 10.7% 2-J.4% 42,8% SS.§% Cmidi1c1M1y . *(~unhos/cm) * "Allmliuity JOO% {mg CaCdliL) J , , ~ U:u*iJ ness '(m 0 .CnC0~). -*TR ehloi'inc.(mgll.), *Temperature (~C) } H {S.U;) DO (mg.IL) Conduc:livity J 100% lnfake hmihos/cm) "'AJl::llinity (n\a CaCO,;JL) *Hardness {mg CaC031L) *TR ~blorine (mg/L) "'Temperature (~C) Finni 1rimn1 Finai. Initial initial , * ***~,***.i. .. 1pcralurc~.p~rfomicd at 1hc rime of!esUriitiation, renewal.Qr 1cmiinalion by*ihc analystid,entined'in:lhe Dail}.,Rcnc\\'al Jnfom1aiion table located on Page; !, ..:";All*u 'nil)'., hardness am! tolal rc;;idual cJ1lorine*perfom1cd by U1c mialyst. idemificd on the*hcnch sheet specific for each analysis and:trnnscrlbed*tg tllis bench sheet . ,'v 'Oy!"',, ~* T?lal 1rcsidt1ill clllorinc w;is perfonned 011 non-lri!atcd Outfall IO I and lmrikc sam)lles. * ~ *Page 35 of 93 _ . SOP AT20 - E;xhiblt A 1203, revision l J-l} 1-14 I Page 7 01'7 Species: Pii:uephales promeras Client: TVA /Sequoyah Nuclear Plant, Outfall HH, IJY-tt'l!ated Oat~~ 07-25-17 Day (Anulvst idcritiffed,for_cncl\ day, 11crfom1cd pH; D.O. and conductivitymensuremtnts only;) t 1 2 'Analyst M.5_ -__ HS ' ] Concentratiorl Parameter pH (S.LI.) Control DO (mg/L) Non-treated * *Conductivity (11mhos/cm) *Alkalinity (mgCaC03/L)- #ffarclncss ,r-- - ~~"-~;,.** *1 i v ' - ._;_ - J-.,-. , __ ""' (mg CaC03fL) ~ ;, .. ,........ "" --~.*~ , .....J .~ _,;: (' "~-.c:-" "Tempcrntt1r:c ("C} Initial [ Fim1l Initial Final Initial. I Final Day fAnalyst identilicd-for CDch.day, pcrfom1cd pH,.D.O. and conductiviLy measurements only.) 3 4 5 6 Analyst Concen- Parameter 'tration H(S,U,) Control DO (111_g/L) Non-trcn tcd -Conductivity '1 (11mhos/cm1 *All;alinity (mg CaCO;/L) "Hardness , (nig CaCO!/L) *Tern erature ("C} *Initial Finni 'Tcmpernturescperformcd at the lime oftest initiario11, rencw;il or tcrrilinaifon by th" Bnalyst-idcntilicil in the' Daily Rc:n_ewal lnfommtion cabldocilted 01i:Pagc l. J Alknlinil}', hardness-and total residual chlorine perfom1<;d by the analyst-id~ntificd on the bench ~hcct specific for each unulysls-and imnscrib~d to this l1enoh sl1ccl hy:~~ - - Page 36 of93 SOP AT20- Exhibit AT20.3; revision Ll-01-14 TVA I Sequo)'idt Nuclear Plant, O*rtfaJI IOI'? UV-h'.entcd July 25 August01, 2011 M /'hm:i1f111l.~.s prnmcllt~ Chrnnic Wb£Jlc Ernucut To~lc.lty*Tesl* EPA~21~1~-02-0J3, Method 1000:1) Daily Chemical Analyses (i11vifoo111en111t T<'Sllng Sotutfons, lnr.. 1'1'oj~c1111m1hrr:. _ _ _ _ _ _l_zs_t1_:1 Dovo llnvl Dav.2 !>:iv 3 0:1Y6. Initial .Initial F'iblll lnitii1I Finni *Initial llintil lniti11l Final" lnllinl Fimil . Initial
- Fimil nlNSUI 7.86 7.88 7.90 7.73 7.77 7.46 *1.39 7 55 . S.05 H10 7.51 DO (lnu/I,) v $,I 1.1 7:8 7,7 7.6 7.0 icl 7.0 7,IJ 7.3 7:3 7.l
{)lnll'HI,
- Cu111l11cth-ilf (nmlm~/cm): 308. JI I 313 317 .323 ' j}l 320 i'i1>it-11~*:1tt1) :\lli111i11il)" (m1fll, CaC0 1) 59. 61 5S llur1lnc~s (mg/L CuCOil S6 S5 Tcmncr:thu*c (CL :N.5 .1~.6 2.:1.7 24.5 ~4.5
. !Oii (Sill ;.gs 7.71 7J!6 . 7;4!1 7.88 7.51) *1,63 7.9.t 7.59 8:03 7.50 OO(mu/Ll 7.7 1.S 7I 7,l SJ! 7)- 7.6 i.O 7.8 7.2 c::ontrol, Co11d11c1Mlv- (ittnhusicml 301 '307 :JIO 31:\ :Ill J!O. tn*.11*c.:i1ed 59: 59 58 ll11r1lncs~ (mf.l/L cncO,j &3 87 83 24.~. 2,1.6 14.3 .2'1:9 . lull !Slll 7.83 i98 7.86 7,39 7.87. 7.46 7.59 7:95 7.55 S.03 7.47 00(1111!/LI 7,!l 7.0 .-s.u 7.1 &;O 7.2 Comhsctivlt,* l1mi1in~lcml 194 306 30S 3111 J{I} J07. Teo\11c1*ut11rc ("( J 24.9 JA.2. 24.8 2-1.. 8 15,0 24.5 14.8 1nll !SUI 7.% 7.83 RGO 7.70 7.87 7,JG 7.~9 7,93 7.97 7.51 DO rnfr*/Ll 7.!l 7.9 7,{1 7.8 SJ! 7.0 8.0 7.1 7.6 6.9 7.9 7,4 C11mludii*ltv fumho~/cml 1&1 193 300 188 296 ~S.ll, 211,9 till !ST.II 7.96 7.88 8.,tll 1.70 7,43 7.SS 7.47. '.i.IJ3. 7.97 1:5'1 7A3 no fmt!/L) 8.0 7.Q 7.7 7.9 <r.*~ . 6.ii 1.6 7.1 8.0 6.6 Corul11c<lvit*; 1timhii.<lc-.u\ JUU 257. 261 170 :!<IS 265 2<l ..5 lnll ISUI 7:SS E.Ot 7.66 7.J9 7.96 7.44 8.05 7.33 l>Olmri/Ll 3.0 7.7 8.0 1,0 s.o . 7.0 $.I tj.J 7.6 7.fl 8.0 5.7 Co111luctlVi1v (umlmslc111) 110 2.15 ::!U6 .216 :!!6 214 Temucraturc l"<'.'1 25.U 2*1,<.i 24.5 15.U :.S. l '.!>I.Ii 2*L9 ull (.Sill 7.'16 7.66 7,Sl i.S~ 1.90 7.J*I 7.96 7.38 8;05 7.30 OOlmg/l.I s.o ~.O S.I 7:S 8.0 s:n 6.8. .3.3 6.0 7.7 6, I Sci S.7 C1111d11ctMI\; ( lilnho~fcn1) 193 195 IStJ 199 .:!03 192 196 1\l1mli11i1y (111~/1,*cnCOJ} 71 73 77 77 *'ru111l Rciililtrnl Chlorine ( 111!!/L .:o.JO. <<O.IO <0.IO. Temnemturc /"Cl JS.I 15.1 24.u 25.0 25.0 11CJ (SUI 7.99 7.~3 7;85 751 7.89 7.45 7.96 7.56 8.05 7.37 DO lmt!IL) *SJ s.o S.3 u *.<to 6,1\ SJ 7.0 8.2 -6.S 7:7 1.0 *&.O. ConduetMlv ( 11111hoslem I 191 19.I . 1R5 . 1o_u% lnlni<l' Allmliiiitl' (1111:/L C:iCO,) 7! 71 nl 7J 'Tntill lk!lfll un I Chlo1*inc ( 111!!/L ..:0.10 ><CO.tu <0.10 25.U. 15.2 25.0 25.0 1'1te: s.;inio1 _072:;i17<::1iem.xlsx En1.::red by; T SleeD.e' Reviewed py: -Jt'.- Page I of7 Ch1*onfo\\'hole EftluentToxicityTcsti(EPA-821~R-02~013lVfotbod1oo:tO) Speeies: Ceriodaplmia dubia Client! Tennessee Valley Autlioritv County: Hamiltoi1 *Facility: Sequovah Nuclear Plant Outfall: 101 NPDES#: TN0026450 Project#: \-i.S& 'S Dilution preparaliou iliformatiou: Comments: Dihltion'pfcp (%) lfl.7 21..l 42'.s: 1!5.6 IOO Effluent volume (nil,) 2675 )35 (070 2140 2500 Diluent volume (mL) 22325 1965 1430 360 0 Total volume (ml..) 2500 '250(} 250(} '250(} 25Dv Tesf.orrtallism source iu(orniatiom Test informatir.m: Or,wnism *age: : < '.N-hours old Randomizing template color: .c:...-..::.t-i. Date and times orga11isms were born
- O"\'"'"&ri Cll..~O ""to Ol\\.S
*£ncuba!OI'. t1Ulllbr.r 11nd sh~(f . between: locntion: . "'1...& \ Culture board:. *Replicate number; m-t~*n I I 2 I I, A* ~ I 5 I r. I 1 .I. .s_ I 9 I 10 Ci1ffure board cup number: i.,,*111 t\I l1l 1.'t 11.b I 1..'*H'?9. I~2..1'3\,; . YWT aalClr: .a'l*~*r1 . Transfer vessel infomi:ition:. I PH = *:'\'. ~k S.U. Temperature= t..'i .o"C . Selei.ms1r11111 batch: Average fl":IJ1sfcr volume (mL): I "'*0.25 n\L cn-o<o-t1 *Daily re11ewiti iltfor.matioll: Dny Testfoitintion and feeding, MHSW *S:1mpfe numbers used An:ilyst renewal and. feeding; or b:itc11. u.~cd Outl1li io1 Jnmke terrnination time 0 07-25-17 07-26-17 2 07-27-17 3 07-28-17 4 07-29-17 Q'\()'0 5 07-3'1-l 7 b8'1..6 7 Comrol information: Sti1m1utrji oftest endpoints: Control-I Control.c.2 Acccptimcc cdtcrin % ofMaleAdults: 01. 01. .s::ZO% 7-day'LCso )' /CX\ 7. % Adults having 3'dBroods: I O<<l"i.. lOO 7. 2 60% surviving adults NOEC l (\"? ~ % Mortality: 01~ c1~ dO'Yo LOEC ) (N'\7. Mean .Offspring/Female' "'l..'i *'\ l..'1*b 2 15.0 offspring/female *ChV ') f N'I. 7 %CV: '1.fi7, *. q. () 1. . <4i.O% . IC1s ') l'00-7. Page 38 of93 SOP ATfl - Exhibit AT 11.2, revision H :o ! *-14 ~ r? c ~ ~ ~--~-,:p1~ I: \:.:.:._: Page2 of7 Speeies;- Ceriodaplmin.tlubin Client: TVA rSeguovah Nuclear Plant; Outfall 101 Date~ 07~25-17 CONTROL-1 Sun*ival mu/ RepJ"oductiou Data Replicate number Day 1 2 3 4 5 6 7 8 9 IO *) Young t)roduccil ri 0 CJ 0 (\ I 0 ,..., C\ a r\ Adult mortalily '- '-- '-* \.._ '- \._. ~ '- '-* \._ 2 Young produced (') 0 CJ L-0 0 L-* ,(") (\ C\ 0 a Aclnlt morlalif)'* L \..- \._ '- L, '- L. \._ ,J Young produced 0 r--.,, 0 0 a 0 0 (:'). l'"'I ~ Adult mort:ility "'-- '- '-- L L. '--- '- \._ 4 Voung produced '4. t...\ ~- s \..\ ~ ~ ' v. s t..:.\ \.._, L-Adult morl:ilit,1* \.._ '-'. \..,_ '- '- \._ '-- '-' s Young prodoccd* \0 l"t) \ \ a,. 10.* ~- \ .,_ JO &'t) r 't.3 Adult morrnlilY \.. '- L \._ \...- '- L \,_. \._., (i Young (Jn:iJ.lucctl D 0 0 ,('.) 0 G 0 ' 0 0 n
- Adult 1nortality \....:.. '- '- \..._ '- \._ \_: '--* '- '--
7 Young pr_o_tluccd i"\ Llg ~::, \ l,g \.~ l. £- \ '"-\ \ "'\ \~ \ \.::. To!nl young rroduccd t.& '?lo L."'l :,o -z.'\ ~L. !,\o ~\ :,~ ~o -\._.
- Fini!l Adult Morlali~* '- '- '-- I*'.\._ \...... '- L. . \._.. \.
.x for arJ 13roods. y ~- "><.. ~ )<. ;><_ ~ ~ x "'.')(: Co11ceJilraJiow % Mortality: ! 07. Mean. Offspring/Ferna!e: I -et."'\ CONC: 10.7% Sun*ival "iul Reproductio1t Data Rcolicate 11umbcr Day j '! J 4 5 G I 7 8 *n JO I:' Young pl"oduc:cd 0 0 a (') ('} 0 0 CJ 0 {-) Adult mor1ams I.._ \__ L L L L \_ L L... '-- 2 Young produced () r, ' 0 6 0 6 0 n . () 0 Ailull mortnlity \...... \_ L \._ L.. L '-- L \....__ L_ 3 \'ouug producciJ ri () ,...., () 0 0 0 0 c 0 Adult mortnlily '- L L '-- L L L L 'L \,;._ J 4 Young produced Y; 5 '-\ s ~ ~ '-\ ....:.\ ~ \.,,.\ Adult mo_rt:ility '- '- L L "-- L \._ '- \_. \.,__ 5 l'auttg produced :fl l1 IQ I l 'O. .!"1.. }~ < \~ t.O 12 1*0 Adnll m11rtalit)* L \._ \..._ L \....__ \_ '- '- \._ 6 Young produ~cd (") C\' () () ; {>'\ () 0 a I ('\ 0 A.duJt*morlnlity '- '- \....... L \._ L. L L L 7 Young produccll 'folnl yoi1ilg product-tl l.~ !:it) \°'
- is.'
\~ o"Z.. 1 l., b\ ~~ l""\ '\ ~~ '\ \1,:. ~~ ~~ \ C\ ~$ \t \~ .~1- \._... L.: "FinnlAt!ulf Mortalily \__, 'l._ L '- n*"o\"ef '-sonn,c umt-11 (O '- I.._ oV.Ci v.-itf1 a(f.u1t rlunilc. lrnru crl. , \. ' co,1ce11tratian: %Mortality: 01. Mean Offspring/Female: .3?...8 %.Reductio_n from Control-I; -lc>o1 *r?. SOP AT 11 - Exhibit AT 1L2, n::vision ll-(ll ~ l 4 i ~ p; HS:>,:.:.!;~ *~ :*? !'! .E .', ~.:~~NJl~J~~~~~ Pngc ~of:? l Species: Cerioilimlwfa ti11bia ~I Client: TVA tSs;guovah Nuclear Plant. OutftilUOl
- coNc: 21.4 % - Sm:vb10/ c111tl}1.epi*otlnctlo1i .!Yara
*Renlic11tc* 11i1111bcr Day 1 l 3 4 5 6 7 s 9 {o 1 Young prodnccd CJ *o .(") 0 t'l .0 0 b {')" ; [\ Adult mortality \...- \..- '--' - \..... \_ \,._ '-- '- ,\..., !' Young*produccd ('.. 0 D* ("\ () ' .6 a o. a a Atlu,f t .mortality '- L. \..... L.. \ \....;... '- "'- \.__. \..._; J \'oung produced a 0 0 ..__ c 0 .o *(j a a .f"'.'.. Atlul! mortality '-- \_ \.._.* \,,;..._, \....._ '- "- '--* \.- 4 \' oullg p1*0<1uc~d '4 ::;; '-:\ q lo '-\ ~ \p. ',J\ '-\ ~. Adult'mortalily L L \..,;.. \,,.... \._ L L ,\,.... L. ' 5 \'oung produced \'D \::S_ .t \ ,. c '\L.. Yl-: 10. \ (.;.' \l . \1-1\dult 1mirtality \. '- \.... ~ \;_ *c*, *\.._' \....;. '-- '- 6 ):oung produced D. .o* 0 c o.* 0 ! 'O 0 - () .() \;_' Adult mortality L.. '- "- ~- '- \- '- \,_ L 7 \'ou_ng produced \l 1.Cl '\ ~* \\o \\o. ' . \ '\ .. .\ <\ l& ""Lo \\, Tat.ii rmmg pradue:Cd !;)"'l.. *~.~-* :;~. eol;) s~ ~s : :.s. '~':\ ~.~ 3'-{ Final.Adi1tt Mortality \.._ \:._.... ~ '-- '- '- L.. ' \.;.... O\'"-" {olE.urin.\?.cmir:d*ovcr with cduft durini:. han1fcr), 1 Coru;e11lrf!ffo11: % Mortality: 01. Mean Off~p;ing/Feinnle: 2>9.ri; % Reduction from Control-I: ... '"'* b 7. CONC: 42.8% S11r:1c!ivc1( mu! Repi*o<li1ctkm Dalfl. Rcnllc111e numhw Day. I 2 3 4 5 Ci i 8 9 IO' I I \'oung produced Adiil! JnQrtalily '0. ~ {} L. 'L (). L () '(j *L () L... n L:.. 0 L-0,__ 0 L I 2 *Young:produccLI .0 0. ("\ *G () *() o* (j' 0 () . Adult mortaliiy L \_. L '--* L L L- L L '-- ,,3 Yonng produccLI D I() D 0 a n 0 0 0 ' (} Adult mortalil)' \,_ '-- '-- '-- \;,._,.., L L L .L .L 4 Voung*prmluccd ' '-'{ \o s .'S ~ "\ 'I...\ ~- ~ .L l "'\. Adult rnol"t:llit)" L L- .*5 \'oung produced l1,_ 10
- 1..
L '.:!> 'L L \, ,,_ \.... 1... l 1.. 0 l:b Adnltmorc:ill!y "- '- .L c '-- \,_ L. i \........ \...... G Young,prodl}ccd rl Q, aL L u 0 L ('i L. 0 L 0 L 0 '\.,,..- .. 0. L Ath11t mortality L \... 7 . Yo_ung prod1iccd Tot.al you_ug.producc(l '"Lt:. '"'2.,\ l\ 'x "2..D \. '( \\ '~ \-\ .~q 1.6. ~\,. :;,.;l -~~' !)\co "&\, ~'..\. .i!J~ ~!..,. ~\ Final Adult ilfortalih' ,"L L L L. \._ l__ c L *L L - aver (om 1snru1r: Qin,,. over wit h " duIl d~inu.l o:i.nsfi) ~i . Co11ce111mtio1i: % Mortality: *01 Mean Offspring/Fem::ile: ~s-.o % Reduction from CC1ntrol- J: ..:1,.~1. _,j Page 40 of93
- SOP {\.Tl l -ExnibitAfl l'.2, revision l 1'-0l-L4'
/ Page 4 0f7 Species: Cariotluplmin rl11blt1 Client: TVA I Sequoyah Nucle<ir Plant. Outfall 101 Date: 07-25-17 coNc: 85.6% S11n1t~*nl imilReprodudioi1 Du/11 Rcplit::ilc number Day 2 J 5 G 7 8 9 10 I Young prodncctl 0 () {"') (')' 0 Adult rnorC:ilir)' \__ \_ L '-- 1 Young produced : a 0 0 0 {'\ 0 Adult"mortality '- \,_ '-- '- '\_ L J Youug produced 0 0 .. Adult morta}ity \....._..' '--: ~ Young produced . =:,. ' '"-\ : s ,_ A!l!llt mortality '- 5 Young produ~c1l \~ \L_ "L Adult mortality '- '- '- 6 Youl)g p1*od11ccd () D a 0 [) .Adult morfality* \..._ '- '- '\...__ 7 Young produced \~ L.\ Totnlyouug.produccd Finni Adult MorllllitJ* '-- '- \.... L.
- C011 cemrmirm:
% Mortality: 01. .Mean Offspring/female: *%Reduction from Control-I~
- 1.\.t\1.
CONC; l00% S11n 1hrn/*mul Reproduction Data *Rcvlh:alc number Day I 2 '3. '5 G s 9 .. JO J YOlll!g produced () (') [) 0 " 0 0 7 (') 6 0 c Adult mortnllly* \..._ L- L '-- ~ \...... '-- \_ L. L 2 Young.produced Cl'. ' - (':') t'i ,-:.., t'i c4'i ("\. 0 0 (') Adult.mortality '- L L* \..._ \_ .L L L L L J . Yoimg produccll (<) ~ ('"\ n (') () 0 0 ('\ 0 1\duit mortality L \_ L 'L L \..._ L L L L .4 Voung. prod uccd. l.. '-4. . <o L\ s 'S s..__ ; ~ ~ Auult morlalil)" \.._ "'-- ""' \._ \_ \._ ~ '- "-- L .5 Yt:iung pt*oduccd \.~ r-z.. t~ '~- \L \.!:) \\ \ u. \"-\ ;tdult m'Jrlalir,* \;_ '- '- L. L L '- '-- \..... L
- r. \'tiung produced r-1 a'-..; a 6 _() (\ (\ 0 0 (l Adult mortnlity
'- ~ '-- L L L. '- L '\.._. -ct. \~ '\ ,_ 1.. '7 Young produced Tol:il young produced "2,\ \ \ *~ I..\ -i.o '& £.ei I..\ 0 .,.,~ -~~ ?_,g .. ~-.;.\ ~~ ~"" ti\ ai ~8. L LI. \.._,;.. \,_
- Final Adult Mortality '- '- \.... L L
.'tote: Achih ~onalily fL n h...e. D,. d~'Old). sn ~split brood (s*nv.lc brgnd :p\a bt;\\_\'l;:'e:J IWO days). co:::. CllITV aver forfs:pnnc; ~rneJ over \.\ith ~dull dutiPt! tr.msfc:r). ~. , Cm1cemfntio11:. % Mortality:. (\ 1. Mean Oflspring/Fcmalc: ~1.~ % Reduction from ControJ.:t: - '21.b 7. Page 41of93 SOP ATl l -Exhibit ATI l.2, revision *ll-Ol-14 Page 5 of7 Species: Ceriodllplmia d11bia Client: TVA I Seguov:ili Nuclear Plant. OutfalHOJ Date: 07-25-17 CONTROL-2 Survflral mu/ Reproduction Daill Replicate number Day 1 2 3 4 5 6 7 s: 9 10 1 Young protluccd n (") 0 0 ,__ .0 -*a 0 0 {). f--.. Atllilt mortality \... '- . {"\ L '-- '-- \... L \.._ L_
- ! \'oung protluced f"\ ('\ (\ (') (\ ("\ (\ (') (')
1\dt1lt ritOl'(:ility l L L L- L- L ~. '- l_* L .J \'onng prmlucctl () ("') c-... ('"\ ,.., () (\ 0 c 0 Atl1ilt morr:ility L \_ L L '- - \.....:. '- \..-: ;j Young*produccil ::s '-\. "'\ '-\. s "?:, '--\ c; 5. '-\ Adult morlnlity \....: \..._ \.... '---- .\,.;._, \..... \.._ '- '- \.._
- i Young protlucc!l ll ~ "\ ra to. 10 l '"\._ 'Cl t \ \\
'\...... Adult tnort:11ily \._ *'-- \..._* '- '- '- '- \.._ (i \'oung protluccd 0 a a 0 0 ("\ 0 0 ("\ 0 7 . Adult morrnlity '- '- '- '- '--* \_. \.._. '- L '-- Young p;-ocJuccd l\o \~ l1 .f~ \~ \~ I\.\ 1"-\ i~ \~
- !:O ' ~Jfi>!. :,!;)
Total young protlucctl ~\
- a t> -Z..'\ ~\,) '1J\ ");;~
Finill Atlnlt i\fortnlitr* X for ~*d llroai:ls f'. \_ ')c:: \_ *'-- x.. X-L L ~ x. Nm.!.: ,,dulr rnonaluy (L""' lt,-c. D:::.. dcid), SO*- !=pin b1ood (smsl~ btood spin ~twern 1wod~ys1. CO""' c.:IT)' ov(f (ofT:;pnng cuni::d over w1th:idull dunns ~r:JrtSftrJ. Com:tti1trntiou: % Mortality: I Ql. . Mean Offapring/Fcmale: I "1.C\.~ COiXC 100% Intake S11rvivq! and Reprodm.*tiiJ11 Data Renlicnte number Day s 0" I :? 3 4 5 7 9 10 I Young protlucctl 0 (J 0 0 () 0 () 0 rJ r\tl111t murf:ilily \_ L L. \;_ L L L L \..... L- ! Young pr-ad11ccd D a. {) 0 /) 0 rl. ("., (") ' ~ Adult m1JrlaliCy \._.. \.... L L L L. '-- L L ' '-- . 3. Young 11rot1ucct1 Adult mo1*1111il)' 0 \._ 0 ('\ L L 0 L 0 L () (") L ('), L () L 0 *I \'oung 11rotlucctl ~ \p \..::. '-!.. ~ ~ '-\ \,p \.o ~- 1\d11lt mortality '-- '- ~ \.._ "-- \..... . \.._ \....': L ' \..... 5 \'oung protlucctl Adult mortnlity \..;_I *~ 1 :::. "L' \_ \ 'S \._ ' \._\ I\ L 'l\ L '~ L I\ \~ G \'*rnng produced ri 0 0 0 6 0 0 0 0 Q. Adult morlality \_ L L_ L L. L L L L. L 7 Young produced l.S;. l 4. li tS \'\. ' \~ \ ""\. LS \'5 11 Total young produce(! ~ ~& >5 '!1"1. '?:iS ?> \ "1. '\ ':>'-\ ~l. :i'-\ Fin~I Adult Mortality \... L. L '- L "'- L. \.._ *over. {oiTnuiru! cariicd O\'C'i wilh adUI~ iJurfr*.<t 1r.irur~r). Conce11trntio11: % 1'1/ortali!y: 07. Mean.Offspri11g/Fcmnlc: 33.~ *% Reduction from Control-2: -r~.~1. Page42 of93 SOP ATl l - Exhibit ATI 1.2, reviSion. I l-Ol-M TVA I S(1q11oy~1h Nuclear Plunt~ Outfall JOI .Jul~* 25- AugmH Of, 1017 " F~:l r--::1 *..~---* "ti Q) Vilrilicnt11111 of n~rimlllplmia Rcprnductlon Totals ~ Ct1vliQnmonu1IToulnJl Sah.111onr1 Int. e Control-I He1'1trturc number 8S.6% Rc:rilknte 1lim1bur 0 co OU\' I 0 I :?_ () 3 0 .J. 11 s 0 . 6. () 7 0 *o 8 9 0 10 0 Tu~a1 0 On)' I' I 0 l 0 J 0 .j I) 5 . 0 6 . o* 7 0 8 0 'J rr** ID 0 l'vlnl 0 w -* 0 ti 0 0 0 {\ a () (*. 0 0 l 0 0 0 (l CJ *o 0 0 0 IJ ll *3 J 4 0 0 .j ,0 I) 5 0 *1. 0 5 0 .1 0. ~ 0. ~ 0 il 0 4' J. 4. 0 .5 n b 0 5 u ~ 0 *I 0 s I) 4 5 0 0 j t1 5 u 4!1
- 5 JO JO II' *9 *10 9 u JO JO Jil 101 5 13 l'.l II. ll H . l:!' 12 I:! II J1 122 6 0 () 0 u 0 o* 0 (1 IJ 0 0 6 0 0 0 II 0 u IJ 0 () 0 0 7* J.l Hi .IJ 16 1.5 JS I~ 17 15 Ju 154 7 18 IS J9 :1 20 . :o. .19 19 Iv 21 l~I Totul' ?8 . 30 ?7 .JO ?~ 32. 311 JI JO JO 297 T"1111 36 37 35 JS 3S 37. 35 Jr. J! 38 362 I0,7% JOO%
ftl;' lliCJHf.' nlunl.11::r 11'!- 11iclll1! 11un1bar Totnl' Do)" "1'11101 Qni I 2 J 4 s 6 7 *8 9. 10* '( 2. J 4 s 6 1 8 9 10 I 0 0 0 0 0 0 () "Q 0 0 er l 0 0 0 0 0 0 (] 0 ll () 0 l 0 0 0 I! 0 I) 0 u Q 0 o. 1 () 0 0 0 0 0 0 0 n 0 0. 3 0 0 o. 0 0 (l I) .'I) (J 0 u .J 0 0 0 () Q 0 0 () 0 0 Q ~ **1 5 4 5 s .. ~ ~ 5 .j' .14 ~ 6 *I 6 6 J 5 5 .5 5 . 6 S'.! 5 *11 11 . Ill JU 1::!' Ill ll . IU 12 JO 109 . 13 12 IJ: *13 . 12 13 II J4 ... 1:! 1~7 (, 7 .() 15 0 J!1 0 rg 0 lb' (l 17 () J~. 0 ltL 0 I'> Q 18' {) IS. 0 175 .6" 7 0
- !I" !2 0 ()
19 () l'I o* .0 JS' :!T. }[) () 0 "12. [) IS 0 .:m ()
- oo Tnl:ll 30 JS 32* JI 34 33 33 .33 35 J1 3:!8 Totnl JO .lH 38 33. JJ 39 36 ..lJ . 37* 38 '379 -
21.4% C(lntrol-2 ~\'\:; )\\\;',\\\} \\Ul\\b~ \\c 1\itnlc nmu\tcr D11J 'rut:JI JJ11i* Tiiluf l ::! J ~ 5 6 T E 9 . 10 I "! 3 J 5 6' 7 s 9 JU I () 0 u 0 ,n 0 0 0 a II 0 I 0 0 (I. 0 0 0 1 .o 0 u o* tJ
- ! CJ (l [I 0 *il 0 !I 0 0 u 0 2 0 .Q (I .0 0 0 0 fl () 0 (!
3 a !l [I () 0 0 IJ 0 0 (J 0 3 Q 0 0 0 fl fl 0 u u n a ~ ~ 5 .t .1 *6 .1 fi ;j J :5 *-l(1 ~ 3 *l 4 4 $ 4 5 5 *I .11
- ._:; HI 1*3 II 10 12 1:. 10 12 II I'.! 113 5 II <J 'l IO Ill JO 1~* 10 II 11 103 6' (} 0 0 (l () 0 () 0 0 0 () (* !I (I ll 0 <l (1 0 n 0 0 ()
7 I" :?0 IS 16 16 19 19. IS 20 17 l>>I 7 16 15 17 13 15 16 14 14 IJ 16. 149 Totid 32 JS JJ Jll. 34 35 35 34 J$. . >~ J~O Toi:1l .JO 28 30 li 30 l'I JO 19 29 JI ~93 42.8% IOO% lntnf<e llny rtcnlicall!'.11U11,hcr Tu111I It!.! ilic:th~ n11111li(lr TDiu1 Do)' t a I 1 11 J ll 4 {l 5 I) 6 () 1 II s () 9 (r 111 0 0 ., I 0 1 (! J Cl J 0 u 5 6 0 7 0 H a .'I i) Ill. 0 0 1 a 0 (J () 0 .t* () 0 0 ()_ u ~- a ll 0 Q 0 0 0 *ll 0 tl 0 J 0 () 0 *o 0 0 o* 0 0 o* 0 3 0 n 0 0 I] 0 u ll 0 0 I) ~ 4 (i 5, 5 5 ~ 4 (1 *1 .1 .17 J 4 6 (j ,j 5 5 4 & 6 *t. 50 5 12 10 12 13 II 12 12 12 10. Hi 11*1 5 IJ t.i JJ 1:; II II lJ 13 11 13. 121 *.s 0 0 u (I ll 0 u u a 0 (1 6 0 0 () Q (l 0 0 I) u I (J 0 7 . ::u ~I I? 'IS 10 11' IS 18* 17 .'.!\1 1S9 7 15 19 . 17 !j I\> 15 l*I 1.5 15 17 161 Tulitf J6 37 Jct* 36 Jo J~ J4 J(, 'JI . 3'4 J5n l'oinl "J! '38 JS* Jl JS JI .:?'/ JJ 32* I J4 J.J.2 ~ ..r..--- . ~'if" @w '..~ t . TVA I Sequoyah Nuclear Plant, Outfall 101 July 25 - August 01, 2017 Chronic Whole Eflluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) . "ti Ill ._/ ;~- ~-~~1 r*--*--A--: Ceriod11plmia du/Jill Chronic Whole Effluent Toxicity Test CQ , I EPA-821-R-02-013, Method 1002.0 CD ' t ....0co / Quality Control (o) Vcl'ilic.atiou of Data Entry, Calculations, and Stutistic:1l Analyses Envlronmf?rital Testing Solutions, Inc. Project n111i1ber:; 12585 C'Qurrnlr:tlion l~cplicnlc number - Sun*fr:ll :\ \*crngc *rcproductiim (:r;erfitil'nl or ~ Percent r*ttlucliuu fJ om ('Yu) (%) (offspring/fomnle) \*:1ri:Uio11 t%J JJOntrtJ contruh: {"Ar, I 2 3 *I 5 6 I 8 9 Hi Cnntfol - I 28 30 27 30 29 32 30 JI :30 30 IOO 29.7 ~.8 No11111plknhle !0:7% 30 35 32 11 3<l )3 r ~ 33 35 ~2 IOI! :n.s 4.9 -JOA* 21.-i'Y* 32 38 33 JV 34 35 35 34 35 34 1110 34.11 6.2 -U.5 .i2.s*1.. 36 37 36 36 36 J.i 34 36 31 311 llJO 35.11 5.0 -17.S 85.6% 36 37 ,~5 38 38 37 35 36 ..,_ ) JS IOll 36.2 5.2 -21.9 1011% 40 38 38 38 34 39 3/i 41 Ji 38 100 37_') 5.2 -27.6 Control- 2 30 28 30 27 30 29 .w 19 :29 31 IOO 29.3 4.0 Nol apvli~al>l~ rno*v. lnrnl<c 32 38 35 3l ~ 35 31 29 34 32 3*1 100 ~3.2 7.6 -13.3 Outf:tll IOI: MSD :. Minitnum Signilic'.ml Dillcrcncc l)u1111cll's J\'ISD l'nlur: LS.17 PMSD= Pcrccnl i\*linimum Sicnilic.*nl Diffcn:ncc l'MSll: 6.2 J'f\*ISD is a mcas1trc ~ftc~l pn.:ci~i11n. The l'MSIH; !hi: 111i1111\lu1n pcrccnl <l11!l:rc11cl! lx:1\l'Ccr1 the CJJntniland IJcatmcm that can bl! ucclnrcd ::talistic:ili~* $i;;nific:m1 in a \\'hole c(Jll1c1u 1ox1ci1y lc51 l11111kr:* l>nn11~11*~ MSll \':tlue: 1.:521 J.owcr PrvtSD bound <lclchnirml by US El'I\ 110'~ pc1ccn1ik) = JJ'l"u. Pi\J!:)ll, 5.2 Urpcr l'/\*ISD bound determined hy USE PA [90'" percentile)= ~7%. towc1 mill up1m l'JVlSD lioullds were dcicrmincd tro1111hc IOih uml 90th pcrcc111ilc, rcspe1.*1il'd\'. ol'l'iVISD llaiu from EPi\'s WE'f lntcrlnliornhil)' V;1nabili1y Study ClJSl!PA 20Ula~ USEP1\. 20011'). I l~F.P/\ .. 21J(IJu * .'.!()()I b. flir111I Rcpon: ln1crl11h11rmf11)' Vmiu\>ility S1rniy ol° lil'1\ Sh<m-i.,1111 Chrome t>nd A.:u1~ Whole Ei'fl11cn1.T .. ~icily Test Methods, Vo!uml'S l :mJ 2-1\pJ'o11di11. Hi'i\-811-B~))-00,l oml IWi\;S2J,HcOl-OlJS. lJ.S Eiwiron111cn111! l'm1cc111>n 1\i;~ncy. Ciiicimmti, 01 I, -~ ..... ' (i....,,.._ d\ TVAJSequoyah :N ucJcar. Plant, Outfoll 101 Jul)* 25,.. Au.gust 01, 2.0l 7 Statistical t\nrilys~ . Cericd>if!hnia .SUrviVal :inc R<>produc=ticn Tcst-Roercllucticn siarroaie; . 'l/2.512.01'1 TmtlD; CdFR~P.- Sample JO; TVAISQN 101 *eiid*Dale:- .8/1/2017* Lab.ID: ETS;Enllfr. 'resting Sol, Sample Type< i;>MR,Dis_charge Monllorln\l R~pcrt S!miple Dale: :July' 2017 Prolocnl:'FWCHR,EPA-821~R-02-013 Tes1:Specles: CO-CE!ifodajmnl;i*d!lbia CommenlS: Non-liealed Cone-% 1 2 3** 4! .fl '6 *7 a 9. 10* Conln?l*1 2e.ooo 30,\)00 27:000' 30:000* 291000 32.000' 30.000 31*.000' '30.00D 30.000 Contt'ol*2 .30.000. :28,000 30.000' 27..boo 30:000 29:000 30.000' 29.000* ~.ODO 31,000' 10!1'. 30.000 35,000 32,000* 3.}.000 34:000 . .3.3:000 33.ooo '33~000 :35.000' *32.000' 21:4 :i2.oao. .sa.lloo '33;000* 30,000 34.000 35:00.0* -ss:boo 34;000 35.000' 34.000* 42:8-' 36.acio .3r.ooo 36.000' 36.000 36,000 34.0p~ *34.000 ~6:090 31>ililil 34.000* 85.if 3EfoOO *37;000 . 35.000 fla.ooo .38,qoo 37.000 35000* :36:000 32;000 38;000' 100 40.000* .:38.000 30.ooo 3.e.900 34.000 39,000 35000 <11 .. 000 '37;000 3Bt000' *lntflkl: .32.000 ~s.~oo* .35.600 32.:000 ss:QaO 31:006 29090 34.000 .32.000 34c000 **.Tmnsfonn: Unlra*nsfOrmod Ranll 1*T'li1Dd *isotonic: .C<>nc.,0/. *Moan. .N-IVioan .M.,an *.Min. Max *CV% ,tJ* Sum Critical M~_:in *N-Mean, CiOnltt'il~l 29>700* 1,0;*37 29~700. 27>000* ::32,000 '4,775" .ip, *34.267* *1-.0000 Ool)ticl-2. 29:300 1.0000 29.300, Gi'.ooo 31.000 3,957 10 10.7 32_.800 1.11.\:ls 32.(:100* '30.006* :35,000 ..1,93-t 10 *146.00 75.'00 "34.261 1.0000 2.1.4 M.000 l.'11~04 ;;.,.ooo. 30~000 :3s.oao *6,201 10 150.00 75.0ci 24.26/ 1.0000 428 35.000 1.1.945 3!i.OOO* *3i.:OOO: 37;1)_00 51040' 1P .15'_3,50 75.00. :.94.267 '1.0DOO as;6 36;200 1.2355 36.200 32:000* 3$.000 5;175 10' 15~..50 ts.oo :.34267 *1.0000 .100* 3?;060 1:29.35 .37.$00' :i1f:OOO 41:000 s.106 iO 1s.s;oo. 75.00 '34..267 LOOOO* mta~e 33.200 1!13'31 33.20tl 2!);000 :9$:000 7.62"0 10 AuxlllaryTcsts StntiStic Critical Skow 'Kurt. Kolmoaorov .O Test-Indicates non.riomiol dfstrib'!1ion* (p .,,. c;i.01) 1:04609 1.035- -0.5496 o.~B5fl:4 = B;!1f1.lett's Te.sl iniilcates equal va1ia11c;:e:s. {P o.a111 . 1:SM68 t~.0663 The ci:>rittol means are not sicmlficanllv dlllerenl-ip = 0.50) o:sso52 2.10092 Hypolht>sls Tost (1-tall; 0.05) NOEC .* *LOEC-- ChV. ;S~l',._Marw-Ono*Rank Tesl* *100* >:100 . Treatnienls vs Control~ 1 Linear Interpolation (2Q(l.Resol)jpll'.Js) Po*nt "la
- SD- .95%*CL .Skew ..
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-D.3 .. Q,*I +-.--,~~.,.-.,.--~~..,.-.,.---i *o. ~SQ Desso/a Ooso;R1>sj:lo_nso Plot* ~s....------------'-------------., '10 ~s 5 30:~,,._-"-i.--- ~ 25 "O ~-~9. *~ta* 10 s* 9:_+. ---...;..._---...-.--_- -.. . ._---.'!'!---..,---0.,------tl~, ~ ~ a;:: eE'. *E !! o
- _11:_
6 6 .*Page 45 of 93 TVA I Sequoyah Nuclear Pfant, Outfall JO:! July 25 - August 01~2017 Statistical Analyses Used for PMSD calculation 0111\'. Ceriodaphnia. Survilial a lid Reproduction Test*Repro*duction. Sia rt Date: 1/25/20~7 Tes! ID: CdFRCR Sarnpfe ID. . TVA'{ SQN '101 End Date: 811/2017 Lab ID: ETScEnvir. Testing Sol. Sarnpfe.Type: DMR"Discharge Monitoring Repof\ Sample Date: Jufy 2017 Protocol: FWCHR-EPA.821-R-02-013 Test Species: CD-CerTodaphriia du61a Comments: .Non-treated Cone-% 1 2. 3 4 5 6 7 s 9 10 Controf-i 28.000 30.000 27.000 30,(100 29.000' 32:.000 30.000 3.1,000 30.000 3.0.000 Conlrol-2 30.000 28.000 30.000 27.000 :30.000 29:000 30.000 29,000 29.000 3'!,000 10.7 30.000 35.000 32:000 3:1.000 3<1.000 33,000 3~>'.000 33.000 35,000 32.QOO 21.4 32.000 38.000 33.000 .30.000 34,000 35,000 35.000 34".000 35,000 34:000 42:8 j6.000 37 000 36.000 :3G.OOO 35,000 34,000 34.000 36.000 31 090 3.\.000 85.6 36.000 37.000 35'.000 38.000 '38,000 37000 35.000 36.000 32 000 3f..000 100 40.000 38.000 36.000 -38.000 34.000 39.000 36.000 41,000 37 000 38.000 lntak>? 32.000 38,000 35.000 32.000 35,000 31,00Q 2~toOQ. 34.000 32.000 34.000 Transform:. Untransformed 1-Talled cone-% Mean N-Mean Mean Min *Max CV% N t-Stat Critical MSO Control-1 29.700 1.0137 29.700 27.000 32..000 4.775 10 Controi"2 29.300 *1.0000 29.300 27.000 31.000 3.957 10 10,7 32.800 1 '1'1$5 32.800 30.000 35.000 4.937 10 -3.837 2.287 1.847 21,4 34.000 1.'1604 34.000 30.000 38.000 13.:201 10 -5.323 2.287 ',8,17 42.8 35.000 1.1945 ::.5,000 3'1.000 37.000 5.0<10 10 -6.561 2.287 1;Bt17 85.6 36.200 1.2355 36,200 32.000 :38.000 5.176 10 'B.Otl6 2.287 1.1347 iOO 37 UDO 1.2935 37 .. 900 34.000 41.000 ~.19('; 11 (I ,10.:151 2.237 1.8'17 lntal<e 33,200 1.1331 33.200 29.000 '38.000 7.620 10 Auxiliary Tests Statistic Critical Skew* Kurt. Kolrnogorov D Test indicateS non-normal distribution (P-= 0.01) '1.04609 1.035 . *0.5498 0.48564 = Bartlett's Test indicates equal variances (p Q.89) 1.68468 1.5.0863 T11e control means are not s!Qnlficantly different (p = o_5.0) 0:69052 2.10092 Hypothesis Test ("I-tail, 0.05) NOEC LOEC Cli.V . I u. .MSDU* MSDp Mss* MSE F-Prob df ounneu*s Test 100 :>100 1 1:84724 0.0622 81.1067 3.26296 6.6E-13 5. 54 . Treatments vs cont<Ol-l TVA /Sequoyah Nuclear Phmt, Outfall lOt ~Intake July 25 - August 01. 2017 . *- . ~; Sta1islical Atialyses Environmental 10?Sllng Solutions *. Inc.. Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 7/2512017 1est ID:* Cd FR CR Sample ID.: !VA I SQN 10~ - Intake End.Date_: S/112017 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Dlscharge. Monitoring Report Sample Date: July 2017 Protocol: FWCHR-EPA-S214~-02-ff13 Test Species; CD'Ceriodaphnia dubia Comments: Non-lreated Cone-% 1 2* 3 4 5 6 7. 8 9 10 Control-1 28.000 30.000 27.000 30:000 *29_.000 32.000 30:000 3:LOOO 30.000 30.000 Control-2 30.000 28'.000 30.000 27.000 30.000 2!thl)OO *30,000 29.000 29,000 31 000 10.7 30.000 35.000 32.000 31 000 34.000 33.1)00 33.000 33.000 35.000 32.000 21.4 32.000 38.000 33.000 30.000 31\.000 35.000 35.000 34.000 35,000 34.000 *42.8 36.000 37.00_0 "36.000 313.000 36.000 34,000 34.000 36.000 3*1,000 34,000 85.6' 36.000 37.000 35.(100 38.000 38.000 "37.0.00 35.000 36.000 32.000 38-000 JOO 40.000 38.000 '38.000 38.000 34,000 39.000 36.000 41.000 37;000 38 000 Intake 32.000 3~!".000 35.000 32.000 35.000 -31.00D 29.000 34.000 32.000 34.000 Transform: Untransformed 1-Tailed Cone-% Mean N-Moan Mean Min Max CV% N t-Stat Critical MSD Contro1-*1 29.700 1,0137 29 ..700 27.000 32.000 4.775 10 *control-2 29.300 1,0000 29.300 27.000 3.1.000 3.957. 10 -10_7 32.800 *1 1195 32.BOO 30.000 35.000 4.937 10 21.4 34,000 1 1604 34:000 30.000 38;000 6.2Q1 10 42.6 35,000 1 1945 35.000 31.000 .37.000 5.040 10 85.6 3$,21)0 "1.2355 36.200 32,000 38.000 5.176 10 100 37,~100 1.2935 37.900 34.QQO -11,000 5.196 10 1ntal<e 33.200 1:1331 33.200 29.000 38.00"0 7.620 10 -4.432 1.730 1 .. 522 Auxllia!_1 Tests Statistic Critical Skew Kurt Shaplro-Wilk's Test indicates normaldlstributie>n (p > 0.01) 0:95353 0.868 0.20974 1.47265 F-Test indicates equal variances (p = 0.03) 4.76033 6.54109 lihe control.means are no! significantly* different(~"" 0.50} 0.69052 2.10092 H~J!Olhesis Test ~1-tail, 0.05) .MS Du MS Op MSB MSE - F-Prob df \ Dunnett's Test indicates no significant differences 1.52244 0:05196 76.05 3.81222 3.2E-O<l 1. 16 T:realments .vs.Conlrol-2 Dose-Response Plot 1-laif, 0.05 !evel of significance - Page 47 of 93 J ~~1::~hlttn~1~~~1~~- Pf1ge Gof/ Species: Ceriotlaplmiti duhirr Date: 07-25-1'7 Client: TVA I Sequoyah Nuclear Plant, Outfall 101 Daily Cl1emistrv:
- Ony (Analyst identified for each dny, pcrfom1cd pH, D.O.-ru1d conductivity mcasuicml!lils univ.)
0 I Z Analyst HJ rlS .HS Concentration Parameter pH (S.U.) 7-flfi, Co11ductivit}' ' - _,_,,. - -* .~""'.:' (µmhosfcrn) sos CONTROL *Al!mlinitv (ing CaCO~/L) ~Hardness (mgCaCOjJLJ "T<impcrature (0 C) ui-I(S.U.) DO(m#L) l0.7% Conducti\'ily (µmhos/cml '"'Temperature (0 C) nH (S.U.) -DO (mg/L) it A% Conductivi1y (Jmth6s/cm) -i<Tempernture (°C) pH(S.U.) DO(m.!1/L) 42.8% . Conductivity * (11mhos/cm) *Temperature (0 C) 111-l (S.!J.) DO (ml!/L} 85.6% Conduccivirr (iunhosfcm) *Tcmpcrnturc (0 C) 'nH(S:U.J DO (mg.IL) Conductivity (µmhos/cm) "'Al kaliuily l00% (mg CaC03'tL) ~Hardness (mg CaCO;/L) "TR chlorine (mg/L) *Tcmperatura c*c) uH (S.U.) DO (mgll) B-' Conlluclivity (µmhos/cm)
- 100% *Amnlinity Int:ikc (m~ CaCO;/L)
*Jlnrdncss (mg CnCO,/L). *"TR chlori111: (mg/L) *Tcrnpcr:iturc (°C) "l5,.\ LS.l ,c;. \ "1.S. 0 Initiiir Vinal lniti:il Vinal lnitinf Finar *1 c111pcratures p~rformcd al the time of lest initilltion, rcnCWill or termination by lhe.nnalyst identilicd* in the Daily Renewal ~")' lnfonnatiun table located on Page I. Alkalinity,'hardness and total rcs_idual cjlorinc performed b}' th<! analys1.idcnlilicd on the bench ,.,,~::::~~-"' . ~[1ecl *peclfic forc:1c11 mrnlysis and transcribed to tilfs bench sheet by: ~~--* ~ Page 48 of 93 SOP ATI I -ExhibitATI 1.2, revision 11-01-14 J~-~:::~,r~1~w 1;::,fl~ Page 7 of7 Species: Ceriot1110J111it11/ubia Client: TVA I SeauovahNuclear Plant. Outfall 101 Date: 07-25-17 l 3 4 Day (Analyst idcnlificd for each duy, pcrfom1ed pH, D.O. and conducliYily measurements only.) 5 6 Analvst Concen- Parameter tration pH(S.U.) DO Cml!IL) Co1fductivily (uinhos/cm) CONTROi;. "Allmlinit)" (mi? CaC03/L) ~nnrdncss (n1g CaC03/L) *Temperature C°Cl 00.(m!!IL) 10.7% Co11!111c1i'Vity * .(1m1hoslcm) *Temperature (0 C) nH (S.U.} DO(mwLl 21.4% Conductivily !_f --v::-,_~- ..~:i ,\ (µmhos/cm) f."-'-", __:_.,..!__:.::.. t *Temperature {°C) pH (S~U.) DO{m!i/L) 4:2'.8% Coml11c1ivity Hm11iosicm) "Tcmpernture c<'CJ ")..S.\ riH (S.U.) DO (mg/L) SS.6% Coµdi1eth*ity * ;,;,:, .... ,~ ..,,.,,
- {µmhos/cm) -1,_*~! ~"~ -:-~~: :11
'°'Temncrature (°C) DO(mg/L) ConducliYity f1m1hos/cm) 100% "Alk.11inity r '~.~~l~j ( r - * ~ -~~~::-"d (mg CaCOi/L) L-*.:~.: ""'-! 1.1'- _. :-:' "l ~Hnrdness (mg CaCO;/L} ~TRchlorlnc'(111g/L) ** . *Temperature (°C). pH (S.LL) .DO(mg/Ll ' conductivity (µmhos/cm) JOO% *Alk:tlinil); lntnkc (Ing CaCO;/L) **Hnrdncss . (nil? CaCO;IL} "TR chlorine (mg/L) *Temperature (°C> lniti:il Finni Initial Finni Jniti:ll Final hiitial Final "Temreraturcs performed at the time of ~t iilililltioi1, renewal or 1cnnina1ion by the analyst identified in the Oaily*Rcncwal lnformatii;m tab(e:locatcd on Page I. Alkalinity.,lmr'dn~ss and Iota! residual ch orinc pcrfom1cd by'lhc ~nalyst identified on the j:lcnch .sheet specific for each analysis and transcribe~ to Ihis bench sh~cl by: _ _,_....,.._
- Page 49 of 93 SOP AT I I - Exhibit ATI 1.2, revh~ion I i'-01-14
TVA I Sequoyah Nucleal' Pinnt, Outfall 101, N1)n-t1*ellted .July 25 - August Ul., 20li Cerimlaplmin t111hht Chl'(lflk* Wholt Efllul~ut Toxicil)' Test F.PA-821-R-02-01.3, Me-U1od 100~.o Onily Ch(~micnl Arrnlyscs l'r*ujcirl 1in111h1"~ _ _ _ _ _ _1_2_s_s:._* Envlr.;imn~ntal Te~lln!) Soluclon*. Inc. Gunccn11111lo11 Puramercr nn,, u D:1,* J D;11*2 D:n* 3 D:w4 IJ;11* 5 Day 6 lnili:il l'iu:tl lnilinl Finni Initial Firm I *lni1inl Fi1f:1I 111i1i.:1I .Finni lnilial Fi11;1I luilial* Final 11lr(Sl!l 7,R6 7.95 - 7:90 7.K3 7.77 7.Sli 7.89 7_lJ] 8..D2 . 7.92 S.05 7.99 g;11 7.91 UO (rnl!ILl 7.7 7;(J 7.7 7.6 7.7 7.S 7.6 ~-0 7.J 7,6 7.6 7.S 7.8 .to ('.0111h1ctil*it1* (11111ht1~lr111) 308 311 313 317 323 J24 . 320 ('tm1rul Mlwli11io* (111g11. c.,co1) 59. 61 5~ I lard 111!!.S (iug!L Cn CO 1) 86 87 SS Tc11111c1*11t11 l'c (C) 1"-1 152 ::!~.6 25:*) 14.8 2~.8 24.S 251) 2;.i.!i 24:9 24.7 25.0 24.6 25.0 lull /Slll 7.90 7.95 . 7.95 "82 7.84 7.80 7.90 7.91 3.01 7.91 3,02 7.97 :i:w 7,89 UO!mwl.) 7.7 7.7 7.b 7.G 7.7 7.7 7.6 g.o 77 7.6 7.6 7,1; 7.6 s.o 10.7% 292 291) JI 7 J09 Coaul11e!i\oi1v (11111hoslc1111 JOI 305 Jill Te11111cmrnrc ("C) 1~.8 14.S. 1*1.7 J4.S 1'1.3 14.7 2U 24.8 .N.9 25,l 24.8 24,8 24.6 1.5.2 nil (Siil 7_91 7.95 7.% 7,82 7.84 7,79 7.90 7110 7.98 'i.91 '8.0T 7.97 S.!U 7.86 1)0/m!!/Ll 7_3 7.7 7.6 7.0 7)l 7.i . 7.6 S.u 7.7 7,6 7.'11 7.S 7~6 8.0
- ll..J%1 C1111duc1i1*i11* (11111hostcm\ 280 294 .19:? 304 JOI .. 191. 301
'J'cnme.nuurc !"Cl 14.8 24.S *24_7 250 2*tS 24.S 2.U 2*1.S 25.IJ - 25.0 24.S 24.7 24.ti 15.Z un !Sl!l 7.92 7.95 7.91 7.&4 . 1.85 7.78 7.'JO 7,S9 7.98. 7;SIJ 8.00 . 7:95 . s:11 7.26 OOhlil!ll.i 1;lJ 7.$ 1:7 i.6 1:3 1.7 1.i> '3,1) 1 ..1 7.6 I.ti 'J.S 7.7 l:l D 4Z;R*Y. .254 Conlluctl\*it'* (mnlms/i:mi 169 164 171 271 264 272 Tem1icrn1urc f'Cl ::N.S 24.9 24.7 24.9 14.9 2~.3 14.X :!-L~ 25.0 - 25.0 2-l,$ 24.7 24.6 25.1 !nil (SU) 7-92 7.95 7.96 7:84 7.85 7.77 7.88 7,89 7.97 7.SS 8.00 7.93 KIO 7.S2 IJO!m~IL) 7.9 79 .i.7 7.6 7.S 7.tl<! -:7 3.0 7.6 7,6 7,(* 7..S 7.7 SJl 85.671i 2U} Conducfi\*it1* f umhoS/cm) 207 211 207 217 21.1 217 Tcmpcro11urc ("CJ 14.S 25,0 20 24.!:1 14.9 24.S 1J.3 24.9 ~5.0 ]5.1 24.9 24.9 24] _2.:!.9 lull (SUl 7. 90 7.95. 7.97. 7,85 7.S6 7.76 7.Sf* 7.SS . 7.96 7.88 7.98 7.91 8,08 7.S*I OOJ1t1!!/Ll s.o 8.0 7.9 1.B 7.8 7.S 7:7 S,' 7.3 n* 7.6 7;9 7..7 7 *) Cooduc1h*i1v hnnho*frm1 194 1.94 190 199 200 i.n 201 1(11)% Allrnliully (111!(/L CnC0 1) 71 ..... 69 1'>9 ll:ardnl"Ss (ml!}L C:1CO,) 73 77 77 Tu!nl f{csitluiil Ghlorlnc (m~/L) .;:0.10 <I). HJ . <0.10 . ~- ..... -- Tcm11c1~1111rc tC) 24.9 24.8 2-l.7 25..1 25.ll :!4.9 14.9 15.0 '.!5.1 ~4.9 15.0 24.9 24.9 *~5.0 oil ISIJl 7.91 7..% 3.00 7.85 7.86 7.79 7.!!8 7.92 7.96 .7.95 7.98 8.01 8.09 7JJ? l>Olm!!ll,) SJ S.0 7.9 7.S 7.fl 7.9 7.7 _::U 7.3 7.7 7.5 7.8 7.7 7.~* Co111luctivi1'* (1111'ilros/c111\ 194 193 187 196 19~ 1as 193 .. '7'1 -* lllll'l';, lu1~hr i\llmlioity (na!(l'L C11C01 l 69 .. 69 lhmlnns (m!!/L C:iCO,) 77 73 S\I To1;1l lh'shlu:1I Chlorine I 1111.:/Ll <O. lO <0.10 .;:0.10 .. Tenniei*:itu1*c !"Cl :?5.t 25-t .'.!4.[, 25.l 25.0 24.'i 24.3 2~.9 _.!jJ) 15.0. 25.I 14_!1 24.8 1*1.il Ffle: sqn-l 01_072517cl1em.xlsl1 Entered by: T.S'f).per Reviewed by. Page &~ Page _/__of _J__ \ .- '-- __...<" *' Enlrlronhl*nt*lle:nlog ,S<>lllt16n>iln<< Toiaf.Residual Chioduc(ORION-l9"77) Matrix; Water, RL = 0.10 mg/L Analyst Dat~*analyzed IL7J"'. * . c)"':J *ZS:--/"J Meter: Accumet Model AR25 pH/Ion Meter lodi<lc reagent: I IJJ/? Acid reagent: . I f'Jl? ..8-=t8t!ff-7 "7 "I ~J Calibration: crf-ZJ?=:l l}.10 mg/L J;OO mg/L mV Change - (suggested rlinJ?e = 26 to JO mV) Reference standard munbcr J)J.C.S /6.;1-S I J.:J.J'.J 1'7=!fJ Millivolts (mV) s-to. "I £t"f"1. ~- -Zt, .. 9 Nore: f'or samples with a residual '. ** chlbrmc 01- > l.O mg/L.. the ~:imp!!:!; must. hc.ililutcd 10 be. w1th111 the i:nl1brauon range . Lnl>orotvr)* control stamlllrd: Reference standard Truevalu.e (TV) Measured value .(MV) % RS= MV /TI1 x.100 number (mg/L) (mg!L) (ncccptabh! range =.90 to 1io%) 1,JJ.J Jl11f!. 0.50 b-53"1 I tr:G-
- 4 '7,..
Sample cha1*actcristics Residual chlorine 'YaRPD = f(S - D) /[(S+D)/2Jl x JOO (mofL) (acceptable range"'+/- 10%) I S ,t, 0
- DOJ..r l, Duplicate l.o. ol!::!3 samo,ie.measuremrmts:
Sample Snmplc ID Siimplc cluiractedslics Residual chlorine .. number {mglL) -~- ~~-- - - - .. - ~ - Reai:?.enl Blank . Lo.ool, t'101t..."-\..0 \, '""' \. SQ.t-J \C'.i' J)./J 1~~ ~--1 rid,,,,. 0-oriZ r1lh'1.L\*ISL- ~* l i-JT1'\<E: .Aro C~c [;) Q.f!Vi "" ' ....... ,,.., * ..., i l / /'I / I J'-fl _jl ./ _ __.., \ / u=r- -~ J - z:J , t /' v Note: .'\II sninplc:; were annlyzed mexcess of EPA recommended holdnig 11m~ (15.mmutes) unless otherwise not~d . Labora((Jr
- c:outro.I standtml:
Reference standard True value (TV) l\1casurc.d \'alue (M\I) % RS= i\'lV /TV x 10() number. (mg/L} (mg/L) (nrccptablc range= 90 to 110%) .. f\)~ V/, .* B 0.50 o. V: 3 Reviewed by I ;t( Date reviewed ~==~,;...~rl-l _--1.S;~-_,_--1_,J-L -_-_-__,-1 Page 51of93 SOP CS-Exhipit CS. I, revision OJ-Ol-*13 Page l,3 l:'age __/_of-'-- ,_ ;*-:=.:J ~, r:,i ~= : : :* r.. _. \ Environmtmral Tentn~ ~olulians. ltica TotaJRcsidunJ*ChJOJ*ine (ORION-1977) Matrix: Water, RL = OJ o mg/L Dale analyzed~. Analyst Irf""' U ~1-:z Meter: Accumet Model AR25 pH/Ion l\foter. Ctilibratiou: !),10 mg/L 1;00 mg/L mV Cliangc (suggested range"' 26 lo 30 m\I) Rcforence:standnrd numbl!r I IVS~ 1'11-e:, IAJ.S.J /o/yf!, Millivolts (tnV) .. 5"'1-->,. I 5'7'H. 9 L_-=l,. 8 Note: for samples with* a rcs1tlual chlorine of > LO mg/L the-samph:is must be*dilut~d l(* ll"* within !he cnlihratio11 range. LaboraliJI]' co1tlrolstmrdard: Reference stnndard True value (TV) Me:isurcd*va!uc (!\'IV) % RS= MV /.TY x mo number (mg/L) (111g/l.) (ucccptntllc range= 90 fo ll0%) }JV.ff J'7-!'d O;SO o. c::>J <f J.os~ ff 7= Dupllcate sam11/e prec/slau: SamplC Sample ID Sarnplc characteristics Residual chlorine %'RPD = {(S - D) l!(S+D)f2]} x 100 munbcr fmg/L) (ncccpt:Jlilc nmge =+/- 10%) r'la'\"z.b .. o \ \\lP\\SG..N \O \ ?alt *-'AJn:i _..,&nM' s L~. oo(o. - -- l Duplicate -* D lo.oo_"'I S amp,I e measurements; Saili(llc SnmpleTD Sample characteristics Re~idunl'ehlorine number (m!!/LJ Reagent Blank ! .- ) o-oos \ 1 tr\ c..r.!). G'l.- T\J~ \ 'S.G_~\Q\- \ "t.l'\"l'l\,.\<:...12. PalP ij--~ ~ ~ O.~DZ 7 / / " / f-1 ,,....__,l / I UT/Joi .A~/ .... Llf v / ' / / Noie: AWsamplcs were analyzed iii ei-;cess ofEP/\ reGomml!mied !\Olding time-(15 minu1e;) unless olhcrwise*notcd, ~- L*alJormorv con tro I ,-;I a1u'/11nI : Rcfct*encc stnndard True 1*alue (TVJ Measured \'<lluc (MV; % RS= MV / ,T'\l x 100 number .(mg/l.) (mglL) (ncccptalilc rnuge = 90 to 110%) }f\ ~~ J1r=IP. {J.50
- o. 5'tt. ldt- ?!?-
Rcvic~cJ by j1--::c\!~,.,.---~J Date reviewed '--_0'1_"..;;.'Zli_*...IJ_.__--'J
- Page 52 of 93 SOP C:S - Ex11ibit CS. I, revision 01-01-13
' Page lo'-/ Page_L.of_I- ~ r~~1 1 -1 <.:., t:c:: \ !, ' .\-------- * ;) Envlronmen.t*ITeiling Solulion1. lnc. Ttita.1 Residual Chlorine (0Rl0N-l977) Malrix; Water,:RL = 0.10 mg/L Analyst Date analyzed : d) *Q.61)* r "\k} Meter: Accumet Model AR25 pH/Ion Meter todide reagent: Acid reagent: _ I\IJ\J.£...-:::iq'1' M/t. &ft Calihmtiou: I O:IO mg/L Loo ing/L m\I Change - (5ugitcstcd rnnge*= 26 to.JO mV) Reference standnrd numbe.:r 1tJ~s \1.nP> \ *N .:> S 141-e ~°'* t.J Mi!livolts.(mV) "1l 2 ?_ (, "" 1. (o Note; For samples with a residual chlorine of > LO*mg/L, the samples ruust be diluted lo" be withi11 lhc calibration rnnge. Laborator comrol stamfllrd: Reference s!nnd:ird 1'rue value (TV) % RS = J\'IV I TV x J 00 .number (mg/LJ (:1cccptnblc range =*90 10 110%) 0.50 redsio11.~ Sample Sample chnrncieris!ics Residual chlo1'ine %RPD "= {(S - D) /[(S+D)J2]) x 100 number (mg/L) l;m:c11tablC range=+/- 10°/o) (t~. (J.t;()I s LD.. D .J Duplicate D Samp,Ie mens11reme.11ts: Sample Sample JD $ams)lc ch:u*:'lcte1*lstics Residual chlorine number (mg/L) - - ~- -- - -- ~* - . . -- - ' R~eent Blank - .l.o. o~s 11-01Z8DZ... ~At.\ ~Gt~ 10\ - . 1~""2. Do JP. 1-tt.n. LJp(P .(Q. () i.8 I ~ ~*. ~ .\.\1/~ ~ ------ ~ ~ -- Nore: ATT samples were analyzed Jn excess of EPA .rccommcnded*holdrng, time (I ::i- mmulc~1 - unless otherwise noted, Laborotor 1 c01itrol s/imtfard.:. True \'nluc (TV} Mcasured.,,alue (MVl % RS'= !\'IV /TV x: 100 (mg/L) . (mgll~) ' . (11cceptabr~ range= 90 tolf{t%J 0;~0 O.oD . Reviewed by lf---...,,V.:P-_*- - - - - i Date revkwed . ;rt: u ~n Page 53 of 93
- SOP CS - Exhibit CS. I. revision 0 I -0 I-! 3
a0 r-> *ic* ,'_:j,.~ 1 * - Page 66
- 1 .....:.~ .! "*:...;,_' ' *,
-* * ... - ---" .t Puge_-"'-_of "-\ - ~ - ~*-.--' ) Envlronmwtal ,le.sting S'31tttlont. Int.. Alkalinity (SM 2320 B..;i.997) Analyst Date analyzed* I0 ~- J:;vL 2 r1-Matrix: Water, RL= .l .O mg CaCO;/L Tifrate samples to * "Time 1.*nitiated' *~-rn~B?...__?,,,.. 11me completed. oc,~:::j '--"~-=--L.------1 pH=4.S S.U. Tr11ra11t uorma 1tv amI mul'/' li/J. rer'l etermr11alt01': pH of i'iormhlity . Nornmlity (N) of n 1so.1 pi£ F;ictor or Multiplier Deionized Tit rant' check Begin End Total = (5 m'l Na2C03 x 0.05)/E. =(N x 5000.0)/ 10() ml sample water refercnca* st:rnd11rd ml' ml ml =0.25/E =NxSOO =4.SS;U, number number CE) . .(acc:cptablc range= 0.0180-0.0:220) 6.0 \h)t1ttM. \J.\SSr~-8'-\ D* \ .\'l:S (1-~ o.o*zo z.. 10. \ .. o.u-O.\ ;..0~1rn1 Luborator~sc;t',dard: l!?I\ Rcfe1*cncc standard True. value Somp!e. / Alkalinity (MV) * % RS =' (\'IV I TV ~- JOO nuniber (TV) volume Begi!I End Tomi: Multiplier. (mgC:iCO.v'L) (ac~cptablc range (mg C:\C03/LJ* (ml) ml ml .ml = 90 to. llO?/o) \~'SS\~~o 100 100 12.5 "~*3. Cl.~ qC\.Ol. lo~l q°' D1111/icale samo.l e 11recisi011:* Sample. AH<:ilinfty %RJ.>D= Sample Sample lD volume Begin End. Total Multiplier (mg CaCOJIL) [{S'* D} /l(S+D)/2)} ~* 100 number (inf) ml ml. ml_ (acccplnblc rilnge=+/- 10%) s U).t-e.n* 6slAJ Duplicntc (B) 100 ~i3 l"sA, -3.f 1*0, \ D 31 .. .. - - - ~ -----* . ..\ _j ~S.Y .~es "5.' ..l *31 -t'-01-Utl-1Hi ' alnx.. Sill"{jce reco11ery: Reference standard Spike y;1luc Sample Spil<l.\ alklilinity (A) number (SV) volume Begin. End Total Multiplier (mg CaCOJ/L) . (ml! CnCOJIL) (ml) ml ml ml I N.5srSS"o 5) llD .J.5.~ '?fb,-)° e. l l()..\ 02. Sample alkalinity (B} Mc;isured spike vnlile (MV) *% R = MV l SV x 100 (mgC:iCOJIL) MV=A-B' (acccptnblc range 1- (mi.: CnC03/L) =75 to 125%) 1 ~-1 61 102-iJJ, SampI e measure111e11ts: Sample volume Begin ll:nd Totnr. Alkalinit)' . Sample number S:imple ID . (ml) ml **ml ml Mtiltiolier (mi? CaCOJIL) )(JO - o-4-15.('\- {)1l-)5u.J ':13.:-5" ~-3 6-8 to~t 5'l a1. o'\o*\"'°1 m'ii.sw IJ\l ) ?l1.~ ws,z. '5'~e
- CS°r
- 04. ill-\""'\ 2 £).t\ s.a '5.6 @=\
er\ - \ L.-\_..., 3 '5.6 . U.":\- ?J,0. <.JD *er\. ~'",:,-l\ y II.+. r~.lJ' 6.0i lt10 "-"' en \"\ .* o \ .\f\atn \ "nd g 2_ l~~l.t .;'l.1'.; ~-'1 l::b) '2.QOO* n tr'i J(h U1- ~ HKu:* D02- I 5'tl 11 ,c; !'A?iL t, 1- lz") 3H . l I()"\ \'1 .4"1 2.. ',.,, *2. '#J. J ;?q I 561 j C.OC} nr.n1<.\. Yo : 3 ~~.l Ul.'i"', 3.i.:I ~ Reviewed by: Date reviewed: [ O"'\*'l.\*\\ Page 54 of93 SOP C6 - SxhibiL C6 . I, revision 01-01-13 ~ i.**:' '1f1~ - -I - .'. --__ Page Pag~ '2- of 6'1 L\ '\-.------ -* fnV"honrncrit~fTe.stlngS1ltl!Oon,,,inc Alk:di11ity (SM 2320 B-1997} Analyst I \)._, Date analyzed .1---Ll).~~-l-.-\)-:--1 Mattix: Water, RL = 1.0 mg CaC03/L Titrate samples to Time initiated Time completed t: ~ n~ll-pH=4.5 S.U.
- 1/rfmf 1wr111a l(J* flJI[,I 111111*c t1111er ~l etermma/1011:
.nH or Normality Normulity (1\~ of H 2SOJ pH Fnctor or Multiplier Deionized Titram Begin End Total -(S ml NniCOJ.x 0.05)/E (fl/ x 50000)/ too ml snmpll! water reforenci: .standard m! .... 0.25/E Nx500 4.5 s.u. number number fE) (acceptable r, - h ' 11 RO
- 0;0220)
'iJ..11-Y L a bOT:lllOTJ' COlt/ro SfOIJtl ilrt.:I Reference standard True*vnluc *Sample 1\lknlinity (MV) % RS= MV/TV x.100 number (TV) volunic Begin End Tot11I Multiplier (mg CnC03/L) (:iccep1:1blc range ,(mg CaCOJ/L) (ml) ml ml ml =90 to Il0%) l N'55\~::, 100 100 :~s ?A.2. ct.r Jo., '4-8 Cte .If). D11p/icate sample precision:. Snmple A!lrnlinity %RPD= ~ample Snmple ID :volume Begin End Tot11I Multiplie1* (mg CaCOJIL) {(S - D) /j(S+-D)f2]} x IUU num.ber f-.JA (ml) ml ml ml (ncccpta blc range-== 10%) s ~ ~ .~ -~ ' 11 lr'1I0 .G"'i Kl\: DoZi '5T i t().j 3ll.Z. 41.~' :i. '-l to.1 z'-1 D L DiJplicnic (B) .) t.\ l.l.p t.N~o 2."f l Z'-{ ~<n..2111-Jilliatnx
- smke recoverv:
Reference stnndard Sp~lt.e value. Sam11lc Spike alknUnity (A) i1umlier (SV) volume Begin End *Tohll Multiplier (mg CaCOJIL) (me CaCO.i/L) fml) ml ml ml \l.J~.~: '50 ({)..) 4\-t,o 41.o +.~ \t>.1 -=1-'5" Sample alkalinity (B) !\'lcasureil spike v:ilue (MV) % R~MV fSVx JOO* (mg CnCOJ/L) MV=A-B (ncccptnblc rang~ (mg CnCOJIL) ,,,75 io*l25%) 2~ 6l lfYZ.O ~/. samv l e mettsriremeuI.s: .. Snmple volume* *Begin End Total Alknllnity Sami1lt!"nu111ber Sam rile ID (ml) ml ml ml Multiolicr .(mRCnCOJ/Ll l1~\-i.,.~.Q 1:1\1/.\ Klf' b02 I 0r -z. 100 0.0 ':t. I ' i.t. f iO.. l L.J\ i'1 (r\ l'-\. "b1 J 3 4.l 0& 4.e 4A \ "'\ t)'.\ 1 (). <fl-. "fVA \i< 1rOOJ\J\l \* 50 . q,2.. Ill ~ \.+. {i\ aLl ,., en 1- i.\1 I '],. I : <f0.'1 \3..e 2.'l I 5q n 0\1'-\. :oi..c ~ 3 1 t-3.s r:>.1 :3,;;, r lb'4-n en , l:l
- G:::l rKJA tu:- 002-I rJ1VV .l ~oo .f:}.\ tt:f .1.I ~.?) 2. ?:>
l1 (r:1,\.., * (,.\ P:> -z. ' lll .1.4 ~~.; ~ .l '-\ '\ 3 .,mt~. ~1 Ji.3. £ :18.3 4.A ' 1..(e \Hr1 n.. 10 If\! Aeuroo'l- ' *t:;D 0 2.0*~ :.e.e 4S i..."2.) C11 Reviewed by:: Date revie\ved: j (T\. '2..\ * \ 1 Page 55 of93 SOP C6 - Ex11ibit C6. I, revision 01-01-13
- Page* LJ?O Page*__i_*-'.of_4__
Alkalinity (SM 2320 B-1997) Analyst Date analyzed*:. I~~J)~*:--'--~=-l,__:-1"-""l-=1=== IA.__, l'vfatrfx: Water, RL = LO mg CaC03/L Titrate samples to Time initiated Time completed:~~ I~ pH =4.5 S.U. T/1tra11t. uormalit11 mu/ multio fer de1er111iu<<f(o11: pH of Normality !'lorrmtlity (JV)'ofH 2SO~ pH rnctor ur i\'fufliplier De1_ *---* -* *-~ check Begin* End Totnl (5 ml Na 2 CO~ x 0.05}/E (N x 50000)/ JOO ml sample wnter reforence -sfandnra -ml 0.25/E Nx500 -4.5 s.u. number number "" (E) *.(accc1... ,. ***~:.. .... lJ',.. 0.0180- 0.0220) . ...;-e11-u rr l aboratory co11tro I.stem<Iarli: l ... Reference standard True value Sample All\nlinity (MY)* % RS= l\'lV /TVx 100 number (TV) {mg CnCOJIL)
- volume . . Beoih (ml) ml Encl nil Tolnl ml (111 ultiplier . (mg CiiCO;/L) (ncccptnblc rnngc
=!JO to*ll0%) . I ~55'~~ 100 100 3Z<3 4'l-~ ~*8 \0.\ C\°\ '\C).61. D:up l"tcate samo Ie prec1.wo11: Snntplc Afkafinit.)' %RPO-- Sam pin Sample lO volume .Begin End Total Multi(>licr . (Pig CnCOJfl.) {(S
- D) /((S+O)t21} x 100
.number 1../A (mil .1111, ml ml (~1ccc.p1<1blc r1111gc. "'"+/- 10%)
- 6D t.ti.3 **1.1c::r ~ \Q,\'
s Q5 *, 1-i. tr\l?..O'° {',i.J ~IY\"2- -z.. t.\1.\.> . .. '\ ..:./' Dilplicatc (8) .l O:o 4 ~tr l D q~ ..-..cJTZU:i-Afi 1 atn:c spine ., recovery:. Reference* standard Spike value Sample S1>ike nll(alinity (A) number (S\I) \'Dhnne Begin End Totnl Multiplier (mg CaCOJl'L) (mi:CaCO.i/L) (ml) :ml* ml ml \~~l~'S~ Jf)O *'&) D.O 'Mo, :). lO.I l'lO °'*"° Sample alkalinity (Bj Measured spike v:dm: (MV) % R ..-MV/SV xlOO - (mg CaC03/L) MV=A-B (ncccph1blc ra ngc (mgCnCOi/L) =15 to 125%) Ci6 q.; 'lS.67. Sample 111ensureme11ts: Snm'ple volume *Begin End Total Allmlinity Samnle number SnmnleID * (ml) ml ml ml Multi1>lier (mg C3C03'Ll 1-i. U1 \ s. o<a li\.J A lu:r DO?; ~ 5U <i).'7 l4.<:; ~.ti rz.I 1at ~°' \ \. u-1' \. t\ 'r1'..J A a.u:' O/J., \ l\ll I tlw\.s l't. ~ '.te C\+ l~ ()"\ l~.a"\ I :z. ~.s 9.tt12.. l..\:"t q~ \'1 <n \ s. U"1 J ..3 ~'{.z. ~q.. 1 '-\ .'\ qq l ~ tr\\\. \ "L.. H"J A f'* 1 i:-ooi .n I ' rm, r *33.1-' 4.VJ q-, nn ,~.c& I 2.- 3!i7- . ;5,;- 4.e '11. 1:1 Ol l ~. 0 e- l --3 'OB1'6. Ut~ Ip 6'. L *10'.?>
- lDO 111n l\.10 'rrt/ft.CW:DOZ Ov I Ll~lo 4'8,o '*L'-1. 0Qi 1 .z.. o.O L.\ ::}* L.\~'".\. . 015'
' ' cn'~.o~ ' Reviewed by; Date reviewed: I 0'\-1.\-l"\ Page 56 of93 SOP. C6 - Exhibit C6:.J, revision 01-01-13 Page u' ~ J; ~\-...* J.tS P.agc, _~Df_v_\_ _ ~ .:: .Ew.1.lrol'\t<<tmta.~Tl'.!o:.\kt'l}-So\u'Jon,Y\~. Alkalinity (SM 2320. B-1997) Analyst Date anal:yied* I *'I),., lf)'.-z.1.1-:r- _ I fvlatrix-: Water, RL = l :0 mg CaC0 3/L Titrnte samples to Time initiated lime .completed I==---~1..1 \~ ~ pH= 4.5 S.1J. Tf1trn11t 11urmarity a11d mt11*c trp 1er <l clermma . I'101r: -Ti Deionized ~T _ Titrnni Normality ;;l1ccll .Begin *End Total Normality (N) of iliSOJ = (5 ml NaiCOJ x 0,05)/E pH Factor or Multiplier = (f'/ x 50000)/ 190 1111 s11mple "'~tcr reference standard ml .- .... -- -O.ZS/E -Nx 500 -:.t.ss.u. ntimber number_ <E) *(acccp111~** *.. ., ,, 0180 - 0.0220) ,...t172{1~ lb n oratory co11troI staml 11rd: Reference sta11d11rd True value Sn1n111C Alkalinity (M") o/o RS == MY I TV x 1QO number {TV) volume Begin End Totnl_
- Multljilicr (mgCaCOiL) (:1cccprn blc range
- . (mg CaC03/L) (ml) ml ml ml =90 to 110%) _\ Ns.\\GS~* 100 100 4Jr \'-\.~ C\e> 10.l qq, C\ctITT. Dtcp ttcatt samo e nrecmon: Sample Alkalinity *%Rf'D.,.. Sample Sample ID volllnie Begin End Total l\'lu\\i11l!~1* (mg CaC03/L) i(S - D)JJ(S+D)l:ll} ,\ lllO .number --1"\IA (ml) ml ml ml. - (ncccptnl>Jc r11~1gl~ =+/- 10o/~) \ i t5"\ \'$ -~\o Cu..t:uo-z., *uu 3 GD ll..\..~ \C\~S' 5:0 {1) lO.\ s~O\' Hx:> *-
- l. 5.0 4 J Dupllcat~ {B) 0
_} A.s 21.t.~ 1Ql, IDD -il if.r 2-1 I )-:- /l{atrrx*spi-,,<e recm*ery: - Rcf~rence ~fandnrd Spikcv11luc Smnpll! Spike alltnlinity (A) number (SV) volum.c Begin End Total Mu It Iplier (111g01CO.;IL) (rug CnC03/L) (ml) ml ml 1111 ~.~sS\"S$o '\DO eo A.S" (J.G,\I' 04.lf l1.) 10.1 200 I 'Sample alkaiiuity (8) Mcasurccl spike v:1lue (MV) %*R= MV /.SV x 100 (mg CaCO;i/L) M.V:A-*B (:1ccept11blc rnngc (in!\ C;iCOj}L) :/S lil 1'25%) 100 100 I CP.lf>. Snmp Ie measnrei12ents: S:iruplc volume Begin End Total Allmlinity Snnmlc number SamnlelO .fml\ ml ml ml Mulfiolic1* {ml!CnCOµL) l"..\(!'\\\. u "flf A r11i:-0021m0J) . 60 ~q.~. :3~.z i.\', ei .,,,,, to.1 q"":}- )""\ lr\\~*cr:\ 1 2. 3~.2 t1Q.-6 '4. lJJ 023. \~lf\ \~.Oj .J .3 ?;de 43.e 5 .. 0 lQ~,\00 ,..., tr\\ \, \'-. ffJA rJ...U:"oo:unl)v l 1..1- '1 g1-s '-l, tA ,. Q; ,.., (.)".\ \~ -~ l z; *~?.s l:tt:,?i '-1.e* ct1- \'\CJ\ 15,().£' l .J Lli,3 1.t~.:, cs.o . 1(;11
- ICO
.,~ Reviewed by: -- Date reviewed:' r a-1-1,\- \ \ Page 57 of93 SOP C6 - Exhibit C6. l. revision nI -ll 1-1 "l () ----= ~ ;, I -:=-::_: I Puge- _ _\_of_Z. __ '\ ------- El'IYilQr'lm'!nl<ttTe.o;;tin9 Scfollohs;. In:. Allrnli'nity (SM 2320 3-1997) Matrix: Water, RL -:* 1.0 n1g CaC03/L D11te analyzed Analyst lr--\J...J=----?J {(.T.;]._Cf,11' Tiln\te snmples to Time frtitiated r__...00:...J*u-3._2____~....j, Time complt;!ted JOO\ '---'-=--'---------' pH =4.5 S.U. 1traflt 11orma iJv a11 d mu l"l" tJp,ter detenm11 nttO!J :. pH of Norm:11ity Noi*mnlity (N) of ll~S0.1 11fl Fnctor or Multiplier Deionized Titrant ch eel< Begin End Tola I =(5 ml Na 2CO,, x 0.05)/E =(N.~50000)/ IOO ml sample water reference st:lndnrd ml ml !Tll ""0.25/E "'NxSOO =4.SS.U. number i1u111bcr . (E). (acceptable nrngc = 11.0180. 0.!1220) L-{.<3 -IN(]..~ 1~~1se>'-i 0.1.fi \?>.2 \'2..;~ O.Olq6 C\,q \t c...+ o,ie- o.i.o.:. o~ rt\ I Reference standard True value.- Sample Allrnlinity (!\*IV) % RS= MV I TV x 100 number (TV) \'OIUm!) Begin End T(ltal Mnltif)lit:r (m~ CaCOJIL) (acceptable rnngc (mg CnCO,/t.) (ml) ml ml rul ";'90to110%} cn-.61. \~I'S$~ 100 JOO 13. 2. ;G.o q~6 c\'R C\1-Dup{icate samv1 e nrecision: S:imple Allmlinity '%RPD= Sample Sm1ipfe*ID volume Begin E.od Totnl Multiplier. (mg CaCO,IL) :(S - D) ll(S+0)/2)} x Hit) 11111nhcr (ml} ntl. ml 1111 '(:1cccptahlc range==: 10%) o '1 *a\.t. 1:t- fr\ \-\~vJ tDu 1'3.0 ~ct.2 \1*2 cv1 s ~l - -- Duplicutc (B) D ..l ~ ;fq:i: 35~ 1.P*L ~ ~I -.l1)z..~1J-1 'atnx. spt e-recoverv:. Reference standard Spil<e vnh.ic Sample Spike allmllnity (A) number (SV) voll1mc Begin End Totnl Multif>licr (111g CaCOJIL) (mg C:iCO.ilL) (ml) ml ml ntl \ ~.SS\~-a 5D I D::i '}f1*2. . 40:3. lL\ C\.O, \\0 Sample alkalinity (B) Mcasnr~d spil<e value (MV) % R= M\l / SV ~ 100 (mg C:iCOi/L) MV""A-ll .(ucccpf:tlilc r:mgc (m~ CnCOJ/L) : 75 10 125%) Lo I '-\'Cl Qf!;,ITT, samp,l e me11sureme11ts: \ Sample volume Begin End Totnl Alknlinity Sample number Snmole lD (ml) . ml ml rnl Multirilicr (mgCaCO.;/L) lf1. a-1. \t {Y'd-\-~w too 4oJJ 1-!~;z. .'5A 4.01 68 I (]"i* IS.*\I M\tSlu UIJ \ 0,Q. Co.a (J. t} I 5ct en -'1.~ ~ I I \ L (/). 0 1'2.o {0_0 6'1 O\* 1:1 - ll J 3 12.0 r~.l\ '5.ot cse "'~\7..'-\..c\ 1 -rJ A~~N*l 0 I ~ .~ I-+.~ 2l.-S- ' 3.\0 (1) f I \-:\ 01 '?Jo .o \. \ z. ;. \,<; acs.o 3:s. ~ 'f ncn"'l~ .~\ J. 3 ~S.u 2(j;~ 3.s 00J \I IJ\"1.'.\.01.. 'i'"J/i~tJIOt lrJ\ \ ~8.5 :;2.0 3~ lo&i \ '"\ Ot 1.b. en... ..\ z '-.* 32.D .35.v 3*v ,.. :fl Reviewed by: I~.--..+J+-\-___,J Date reviewed: lro*-i.'\-l1 Page 58 of93 SOP C6 - Exhibit C6.J, r.::vh~ion 01-01-13 Page :2- of 2 ~ :t:::-~tal;.:ngS,ol:n:I~<;, Ailcalirtit)i (SM 2320 B:.1991) Matri;x: Water. RL = 1.0 mg CaCO;fL. Analyst I ,}-:-- .Time initiated .,j"'-==-- iii'-° r bate analyzed <fJrd..Ct;) r I Titnttesamplcs fo pH= 4.5'S.U. Tnnc completed ~ Tilrrmf11Qmmlit1* mu! 1ritilti lier deter1i:fmuio11: .Jlff Normnliiy N&rm~l!fy (IV) orfl2S04 pH F:icfor ur i\foltiplic:r Deionized Titrant = (5 ml. Nn2C03 x 0.05)/E = (N.x 50000)/ ,JOO ml sample wnter reference =0.25/E =Nx 500 =4.5.S.U. number able range= 0.0180 ~0.0220} lnborafor11 co11trol stamlard: Reference standard True value Samjitc Alkillinity {MV) % RS = MV I TV x. 100 number (TV) olumc Begin End Total Multiplier {mg CaCO,)ll_.) (ucccptnbli! range (mg C11C03/L) (llll mt ml ml , ,,. 90 to ll0%}. \ ~s~l';.:o~ iOO 100 ... ~::l ,Ip LIS.~ L'.'.\,a q_q* q..,_ q~.D '/. recisfon:,. Sample Alkalinity %RPD= AS:lmplC ID \*of um~ ~cgi11 End Ti:itnl Multiplier (mg CaCO~tL) {(S- D) /l(S+D)/21} x I 011 (ml) ml ml ml (iu:cq1rnhlt rn11gc=+/- 10%) -~ s ~\}.l'fk). ~ 5b o~o 26. :5.S- C\..ti Duplicate (B) [) j 3.s ~-0 ~:'S \ ~'1 ~o~~n-s .1vllltri.t. ~Ike recover 1: ~Ci .* Referericc*st1u1dard ' .Splice value Sample , ~pike alkalinity (A} number (SV) volume Bogin End Total Multiplier. (111g CaCO.i/L) (nig CnCO.i/L) (ml) Jill ml , niL a.J ~.s Smupic nll<nlinity (B) Mens1,frcd spike Yllluc (MV) %R:= MY l SV x lOO . . (ii1g C&COJIL) , MV=A-B (:icccptablc range <(JJ\ CnCO:i/L) ""-75to125%) \OJ, tf). Srm111 I e measurements: II .Sample volimie : Begin .End Tot:1I Allialinlt:y Samule number Sample ID "rill) ml *mr ml Multiplier (mg CnCO;IL) 1'JA ~lO\U\J 1~'*0 -:}-1 f\D ~.!ti (t'\ C\°t '"" (l"'1'1..\:\ ** 0 \ \.""\Q""\'1,.lo
- 0.' i
'2-1-s.0 l"S.IO 1q.1 3.~ I ~'1 .t .3 J:s LI.Cf \ "ltt:\7-fc*O \ 1 l~*I* ~~(o I \Cl-:\1;,'ol,.U'L- fJ:A ,SQ.¥\.UCI\ 1NfW \ ila.y ~t,,.2 3.~ 1-'l . ,, '"' en".2.ic* IS'- .\ ::i. litJ.:;J;.. hA*S 3.v 1-' I l'"\ ()\**t ~. 01 ] ..3 ~Q.0 .33:'--' > 3.v . I ~ ~ _..,,, al Date reviewed: I *01*'C:H1 I Page 59 of93 SOP C6-ExhibitC6.J., revision OJ-0!-lJ ti i*',{ l!iJ< *' (:~* Page z::i l' -- **,.. Page l of _:f __J '\ ~" fflV°1fOOl'JWOf.'JITr~iif1~S9i~\f~11s, Jn.t* Hardness (SM 2340*C,.1997) RL = l .d mg C~COJL Analyst Date analyzed I A-UJ~\rvCJ I .:rime initia. ted T1111e completed* I %~5\ . 43:,: I Ti. .. l'itrnnt Norm1\lity* check Begin* End Totnl' No1'mnlity*(/V) ofEDTA pl-I 'Factor or Multiplier r~fcrencc standard ml ml ml =0.2/E . =(N ~ .50000)/50 nil sample number number (E} (:1cccptn hie r.auge = O,O 180 - Q.0220) =.NxJ0-00. \]\)~03 lv.J5S'-l8't o:o fo.o *o.o* -rJ:o200 '"U)Q Lcrbontforp co11trol stamlanl* Reference st1mdnrd
- Trui.: v:ilue Snmple Hardness (MY) % RS =MV I TV x 100 .
number ffV) volume Bcgir E!ld Total ~1\llti(llier (mg C11COJ/L) (ni::ce[ltn hie range (mg CnCO.i/L) (ml) ml ml nil =; 90* io HO%) ~ l l\.15<::.1<00 40 50 .\b~u 0-o -i...o 1£>.D L-{Q ~CQ.O). D1111licatesam1Jle orecfsio11: --*- -* ... Sample* *t*lardness %RPO= Snmplc- SamJ)le ID
- voiuine Begin En<f , Tot1il' '. Multiplier (!ilg ~n(j:Q;/l,) {(S- QI l!(S+0)/21} x*lllll number (ml) ml ' ml ml tf1',0r1- ~St}..) lQ'Q '
1:2..o l~*O t.o -it>~o , *s.nJ \ ~- Duplicate (B) j j\.f.O tro.o "J.O ' i Do_(;(.) -"CR20t'} Reforence.st:ilidard Spike Vali.lc Sain pie Spike hardness (A} number (SV) volun1c Begin End Total l\<lultiplier ('mg C11COj/L) (mgC:iCO.v'L) (nilJ ml , ml *nil \ t.,Jss. rsee 40 51) l'-l*O l6},Q . 1..to. zo.o ea _Stimple hardness-(~)* Measured s1iike value (MV) % R~MV /SVr.100 {mg entOJJL) MV=A~B (i1eceptilblc range (m:::.CnCOJIL) .,,. 75 10 125%) 40 '10 lQ9.6). I s -* -* - --- 'S: Samt>le volume Begin End Total Hardness I Snmnle number TV=ND Blank Samote rD (shoultl be ""0 mg. CnCd.:l!:.}. . (ml) 6b ' *Ill! (}() 0*-0 *ml ml 'o~o Multlnller z,.u..n r (mg Ca0J/l.) ktO f-hS1'1- ifYl\16\AJ lAO '. .ti'l.3 t.\.3 0V C!"'\*0\;4\ "\ ' rt} \.\$1..0 uJ j ' a~ '3 ~~.5 '"LZ.. ©4 C;f"'\*lO*\) )... ZwS 2>0;6 4.3 Sv 0\-\1.*\~ 3 3o.e &1.lti Y* *.r ez. cr\*\~*1 ' - :. A Ll *. . ~YPt* . .z. Lt.3 ~'t 6to 1 \°"'\ er-\ \ ~
- c.0-. ~K\P bo1- I *1s '9St.z. .~o,z.; Lo l z.") '40* 0 '
\-, *<nn A"1 I. .z yo;'J.. <<-t~I l.'l 7\p n l1'\ *.\ .*~\,.,. ;../L,. J -3 '412.rt c;!4.2_ Zwl ' 94 t<it: O'J2..: ~-I- t.\~, ,Q;f!J \,_ O""\.\O .(f1.:) \ .; ~'4.2 ' 3z. Nol~; rr '>I Sml of litraut!S-. used. smnplc*mu~t be diluted: Page 60of93-Reviewed by: I 6C* I D<Jtc reviewed I 6"\*~*1"\ SOP C7 - Ex!Hbit C7;'1. revision Ol*Ol-13 Page 2.B r-~~e __.2_o f J* I ~-- ...~ , Hardness (SM 2340 C-1997) RL = 1:0 mg CaCG3/L Time initiated r..........___ CJ1'~ Time completed ~ r;1trm1t 11orma ,. t~p tl11lf m11/.. '/10 l'1er c.Ieter111mat1011: Titr:mt 'Normnmy 0 -oin End' Total Normality (N) or EDTA pH Factor or .Multiplier reference st1111dard in! .*m1 0.2/E' (N x 50000)/ 50 ml snmple number *number (E) (acccptnu*", ....., - "'118(1. 0.0210) Nx 1000 *"7 Cf)-..DI:} Laboratoni co11trof.stmufiml': *Reference stnmlm*d 'fruc vt1luo S:unpfo Hardness(MV) % RS*= i\'IV IT\' x 100 number (TV) volume Begin End* Total Multiplier (mg CnCO.YL) (acceptnblc rnngc (mg C:iCO;IL} (ml)* ml ml ml =90to lt0%) 50 ,1 oo~<D. 1~~1soo 40 .450 Lhb l;.0 i ,, ~..,.a ~o D up,l"rcate samoI e nrec1sio11: Sample Hardness l'oRPD= Sampfo number -10 Am11icJD volunle (ml) Begin ml End ml Total ml Multiplier ) (ing C11C03/L) ((S- 0) /l(S+0)/21} '.\ lllil s \~61 \'1;.s\C. \( l t:°Pf"9 ' iJ1 '2 2~ 5.o lti.O l10 ~ 20.u t..lo *. lSuplicatc (B) D
- \. -4 lo.O °i-;,\ l. \ .\ ..) '-14 ct.s*1.
J11: * *SPt.,,'e ' aln.t reco11erv; Reference standard Spike value S:irnplc: Spilce hardness (A) nu nib er (SV) volume Begin End To tuI Multiplier (mg.CnCO.VLl .(m~ CaCOJ/L) (ml) ml ml ml \ ~5'~~00 f(y.::> 2..:; lo.O Q.2 3.1.- ri) 2).0 l3b Sample hnrdncs5 (B) Measured spike 1;alue (MV) %R= MV /SV i< 100 (mg*CaCOv'L) MV==A-B .{:1ccepl:1blc range (mg CilCOJ/L) = 75 (O 12,5%} '1'-l ~ tti::t.~'"I. SampIe meas11re111ents: Sample volum!l *Begin Erid Total Hard.ness Snmnle number Sam rile.ID (mD ml ml ml Multinlicr (mg CnCO.JL) TV=ND - D . J.should be 0 m~ CaCO.;/L) **lF;Zt.i~ 1, ,.., (j\ l ~. ~.,i :tl}< \t:°"001.\}J\ ,3 26 'c},z. lo.Oi '*1.+ ,gJ :tn.o lP-6 \ -\ tt'\l t> .tY"l) ;l'<"Kl,. t:' 002v'1 \ IQ.q .11,C\ 1-0 ' '-lo .\ \* O"'\ \-Z. *\.\., I ~ ~Ci
- l~A '2r0 e>o
,., en i-4* ~\o ~ J .3 I l3.q i1.a.o '2vl Qi-{ .,,en io .~J ~'i\ tF" 002* .rJr vu*\ Up.() L I0*1 o:r ze ,.., Cr\\1... AQ I z. !\{.?;+ l+.e> La LIY \ \ \l"\ '""' * : . \ j ~ 1"1.8 \C\; (0 1.e °l'2 \1 (l""\ \ \. ) l) -(,)Ar11~ ooz.. t I IG\.~ -ZZ.6 2 .er lilo
- IZ.o
*., en l~ .c)c.,,. J 2.. ' 1-"2;"5 ~s.H . 3.3 1 l.32..- \3b *Notc:Jf>l3ml oflltraul is nscd ..satf!pk 0111~1 b~dilulcd. Reviewed by: ~
- Page 61 of 93 '-------'-1-"'----'
SOP C7 - Exhibit C7. I. revision 01-01-13 ~ 1:*1 . ' t _ ' *(' ~ '.' z.q I ,*--*~ - P:lge_.3_of " *- \ £0v11unmer.t:ttTa'lilil'IOS0JmJor*r.lrt'* i-Iardncss (SM 2340 C-1997) Analyse I l!J}.Bio ,n-- })-...> RI..= 1.0 111gCaC03/L I Time initiated ~ cf.)1Dl:r Date analyzed Time completed: ~ I 1 rtm t 11or111allIJ' muf 'lmt1ftp, Tft c
- ter tl eterm/1wt1oll.'
fljormmuy _* __ : *n,
- Titrant. Eitd- Total Norrn:ility (N) ofEDTA pH !?actor o.r Multiplier reference :standard ml ml .,... 0.2/E -' (Nx 50000)/ 50 ml sample number number (E) (ncccpt:1b1c ntuiii: -{MH-Sll...:.0.0220)- -Nx 1000
- 'V :0"':!-ZOtt-
- l. LtcbontfOl'J' co/lfrol stiurdtrril:
Reference standard True value : Sample *Hnrdncss (MY) % RS = MV I TV x 100 nurnber (TV) volume . Begin Erid Total . Multipl,ier (mg CnCOJIL) (nci:eptnble range (mg CnCO.i/L) (ri1l) ml ml ml =90 to ll0%) lNSSrs*oo 40 50 i.:;,e '21-.0 /_o., UJ,o '40 .J(X)O) flp11cate samp e prec1S1on: S:unj>hi Hnrdncss o/oRP.D = Sample .......,... snmpfo JO olilmc Begin End
- TM:1l Multiplier (mg CnCOJ/L) ((S, 0) /((S+b)f2l} x t 1)(1 number *1.'fA . (ml) ml ml ml .. --- -
I °' tn \'it,C>~ CL<.1= l:::fu 3 '25 2.1.e 5L2 ~. 'i Z.) -ze.o s l31P* I ':b* .. - - ~ ..j '3Lz. ~tt.~ 3.~ l 1 D'~-\YQ Duplicate (B) ---~ion .Ma tnx . sm.,,re recoi*erp: Reforcnce standard Spike \'alue Sample Spike lmrdncss (A) number (SV) volume Begin End . Tofal Multiplier* (mg CaCOJIL) (mg C:iCOJILJ (ml) ml ml ml l~~l~OO . Bu 'ZS' 31.2- . ,8/,,(o ~*~ "O* ~) z.o.o*. zz-o Sample hardness (BJ Measured spike vahie (MY) % R=MV /SV'x 100 (mg CnCOJ/L_l MV=A- B Cacccptnbh: ra11gc (mg CnCOilLl =75 to P5%) (\..{() ~o. .lOO.OJ, s<lfllfJ,l e 111easure11u!llt.s: Silmjlle vohmie Begin Eiid Total Hardness Sample number Sample ID (ml) ml ml ml 'Multiplier (ml!.: Q1CO,iJL) TV =1~ Blank . (should be -.0 mg CaCQ3/LJ \-un\\.\\ ' 1\J A Cur Doi i -Jf'\ '2~ a"'*!O aq;2 ';Z.. ~ ,~ "tob .. f 01.f .. !'Co t"\ (j\ (;'9\ Cl'\ \~.(I"'\ \ s .Cf\ ,l .3 .2 3q,z. 41.'l .* z:+ \.U .'J 4':1.ID J,). 1oe, t.ao JOB .. uo \ ""\ (J""\ \ \ *.\ "L .DJf>t{JJJ: 001. JD \ ,~.e .Oi.~~4 Z-v ,, o4- .ico l "'"\ Cl'\\'~* \I if' I :2. 2'2).j p;,\ 2.-;- 1oe. 110 t-tG"'\\~.o~ l :5 2.J;.J n.~ 2.e 112.-~ llD \'ln\\,>n IJAw.rooi.vv t ~'}.4 ?Jo.Cl ZlO t20 \'<n \!:J. ob .\ 'Z- 2-0.4 34.2 3.*11 l3i.- 1.30 \'l<S'\\S.ot:. ..1 -3 ~ ,,.t. .~t.'2- '3'+..'S* 3.3 I . 1?12.- l30 Nore; If >1'51111:;if1itrm11 is oscd, snroplc must be diluted. Reviewed by: ~ Date reviewed .1 u'\"to"i"\ Page62of93 '--~~__.._,.~~ SOP C7 - Exhibit C7. l, revision 01-01'-13 ., r:--iL-;;:::J f.; I' .-- -- ....~ (* * ! I .*~* *-~ . . Page oD ,_ _1 __ L _.., Page -W - -of ~ ) 2"n"1t<<lnment1ITit!nfq_gSfilutlo1a,htc:. Hardness (SM 2340 C-1997) Analyst Date *analyzea t--~..., l W ..,......-20-,l-')---i RL= 1,0 mgCaC03/L Time initiated E?~;- Time completed ~ 71"11ra11I 11or1m1 1 11 "m 11111 IP 1er ~ eter111111at1011; Titr:int Normality cnec.- - Encl Total Normality (N) ofEDTA j1H F:ictor or Multiplier reference *standard rri1~ hfi-- t---!!!!.._ =0.2/E = iN x 50000)/ 50 ml sample numb cl'. number (El ,.. hll.'rarigc 0.0130 - 0.0220) N x 1000 .,;.<f1?Z)li-L" borflI ory colltro I stumInuI: Reference standard True value Som pie Hordncss (JVIV) % RS= MV /TV x 100 number (TV) volurne Begin End Total Multiplier _(mgCaCOJIL) (ncccptablc r:mgc (mg C11COllL} (ml) ml ml n1I ='lC\to \10%) \ t\)6S \Sd0, 40 50 31."S 3q;~ .z~O 1.o.o ~o 1co.<1> . Dtip,licate samofe nrecisio11: Sample Hardness %RPO= Sample Snmplc JD vol11n1e Begin End Total .Multiplier (mg CnCQj/L) {(S'-.D) /i(S+0)/21} x 100 nu miler "'r-M (ml) ml 1111 ml I l""\.tn\\.\\' ~UFOO'ZhS\\k) \ is 'bt3S ~IJ.' 2.IP ~) ?.O.o s JOL/- .IDO 1 .Duplic:11c (B) ..1 ~1.1--1* ~8.)- !li:.1.:4 j 90i. \00 --\l IA- :w.r-J 1ftlrl...: SPI e recover11: Reference standard Spike value .Sample Spille hardness {A) number (SV} volume Begin End Total Multiplier (mg CaCOJIL) (mg CnCOJIL) (ml) ml ml mi ... \N~6,~e>e .. *ec> z:i;- i\o*l ao-r .... ~ ~) ~~o (QiO Sample hnrdness{B) Measured s1)iltc v;iluc*(MV) % R =!VIV I SV .x 100 (mg C:1CO.i/L} MV~_A-B (:1cccp1nblc range (mg CaC03/L) =7511> J25%J loo 00 f(X).ifJ SampI e meas11reme11ts: Sample volume 'Begin End Total Hardness Snmplc number Sample ID (ml) ml ml ml Multiolier (mg CnCOJIL) I TV=ND' Blank '*' .. ... .vJlul* ~ * <f.} .?. on- ' ' G1.' :,.en rr~ Aw.Fooz,' ,.sN\I :z. . 2-s io.~ 335 28 /"}\ zo,o ll2.., llo I \"'\<r\\c; *. 0'1 J ~ ?>:>. S'* :3\o.'l 2.~ .( *tllt' .12.0 \"'\ ()\\\
- l'L i-r"A~IY2..* .n 1]i) \ ~...1 ?f?;i) 2, ~ '0'-1.100
\\d1\~.of', z.. 3'10 41~ 1.1 'lag. Ho .x1 crt '~ .Of l ..3 '1 L.fl.1- 4tlS *::z.B \ 1\2- \JU ' ...,.rn1:- L.--- ---- -- ' ~*~ Note: lf ;.ol:!ml of ti1rnnt Js,uscd, sample must lie tlihlt*:d. Reviewed by: :<\ Date reviewed j cr\*'lO-\""\ Page63of93 * .__~~~~-----' SOPC7-ExhibitC7.I, revision 01-01-13 .-," r--:ii-** I --* i_,_. -.-. .;;.'.J . ,......-.., Page 31 i . ' " i ~:-* ;--... ~- fnvlronmenta1,TC!tff1!15clu\fnn~ .. 111c... Page l of' 2-Ar1alyst Dateanalyzed * - - t.L,. Ioi:t.g.Ci,n: ---, Hardness (SM 23.40 C-1997) *RL =. J,Q rng CaCOJ/L 'Time initiated Titi1c compl¢tetl f- Q~S5. ()~ 2.$ *1 Ti I: */ i1mltinlicr tf,
- Ii" Titrnnl 'Normality checl< Begin Encl Total Nonnali(y (i\~ oHmTA pH Factor or l\'ftl.ltiplier.
reference standard 011 ml ml =0.2/E = (N x 50000)/ 50 nil.sample number number CEl (acceptable l'angc = 0.0180 - 0;0220) =Nx.1000 \NUo'D I .>.}6S\ '*t.£31- O*O ~-~ l\.t\ 0*02.C2;. ' 1;.o.z. L<1!1omtor11 co11trol sta11ilard: Reference standard True vnhic *sample Hardness (MV} % RS"' MV ITV;" 100 number (TV) volume Begin Eiict 'fotal Multipli.er. (rug 01C03'L) (ncccptable i::rrng 42.<1. , L'l ~ \ 10""'\1.~*0\. ~ J ~Pl i..t'-L B l.'1 +l \""'\ Ci"\~'{
- Q'1... -fJO 50.r-l10UtJT i Ll\J~0 'llD':f I; 'l ~
\ ., a-\ 1.(,. Cl-Z.. -!.- 2 .. 'lv1 *~eJ.S '*fl - :}3 Note: If >!Sm! oflilrnm is uscd,*snmple must be clilutcd. Reviewed by: I ). ( J Date reviewed [-m01*1.C\ ..\ 1 . -, Page 64 of 93 **
- SOP C7 - Exhibit C7. I, revision O1-01-l 3
~ rd*-,.~,.'Q Page 32. -_......,_ \I I __.,:.,_ .. - Pag_e -2..i_of 2 L.J \------- -! {'1\'ira11m~ritatTCJUng Solulif~nt."lnt. Hardpess (SM 2340 C-1997) Analyst I t,>-- .:1 Rl. = 1.0 mg CaC0 3/L Time initiated 1=_____ ~t-Date analy~~d ===LA:'*=*=~=-~=- l. r I *=lt=*==- Time completed .__ ~ __, T."itrm1J ncrma ily am 1m1 11/J rer t. elermmut1011: Tltnm. ~ *"":* -**--k Be!iin End To till Normnlity (N) uf EDTA fill Factor or Multiplier reference shmdard ml nu -.,._ t-:---_ =0.2/E =(N x 50000)1 SO mbmmple number number IE-) (:tee~ ... , - ... uoc 0.0 I 80 - 0.0220) Nx 1000 r-tt.Y1-~lt Lilbornton* co11trol stnlld(frt/: Reference st:mdnnl Ti-ue vnluc Sample Jfardncss (MV) -%RS;;; MV /TV x 100 nunibel* (TV) volumo Begin End Total l'flultiplier (mg CnCO.VL) (11cccplitblc rnngc (mg CaCOJ/L) (.1111}' nil ml ml =9(110 110%) l~~iseei 40 50 ll\:~ ;\(.l. \ .01 . 2o.2, ~e q2.s*1. D lf{J,r/Cale S({lllf)I.e f}Tf!CISIOll: Sample Hardni$s %RPD= Sample ,sampleiD volu.me Begin 1-End Total i'i'lultiplier (mg CnC0.YL) {(S - 0) /((S+D)/21} x'IOO number K:°v'A (mil ml ml ml s &:\, 14'0""\'1.~ .Ql ~MD\~N "":i 2-S 0.1~2 :;\3_'4 ?___? It;i.") 2-Q. 2- .1 Dllplicate.(B). j iia..~ Zs.~ 2~.l ~ J D 65 4.\; *1, !viiutnxspr* .,,te recoveJ:11: .Rl\ference standm~d - Spike value Sample Spike lmnlness (A) 'number (SY) volume Begin End Total Multiplier (mg CnC03/L) . (mg CaCOj/L) (ml} ml ml ml lA)~\s©e 80, 2~ A~'i ii-s ~.it ~J '2o.2-. ri-o Sample liardni!ss (B} Menso.red spike aliic (MV) % R=MV /SY x 100 (mgCaCO:JL\ MV=A-B (acccplablc r:mgr (mg CaC031l.) =75 ro 125o/o) e~ ec::; IOa.-il. SltlllfJ I e 111e1u;ureme11ts: Sam pfo vol ti inc Begin . End Tot:li Hardness , Samrile number SamoJcID (mJ} nll ml ml Mulliulicl" (mg CnCO;)L) TV=ND B.larlk *."'l.. ,,_, ~ ~ 'tlg-Ea ' -\;L01a11':\ . \"'\CS'\i.i.\,.o\ -WA 5&~lD~OU l 2-5 ~7.15 M.3 l-0 (~} 'Zo.2 q~ I \""\. f.l'""\1Jo.o \ I 2- ~<\~-; 31;2- \,C} ~ \.., 0""\"1:.&*0 \ j 3 61,i.. ~3J \ *°> +:l '"."'\ c~n.\..\ .<n.. 11:~$9...._'101 b~\' IJ ..> I 33.\ :35.i.J \, °l -:r~ \ ""\ (J""\'1.k.. en.. I 2. 3S,l) ~li:i-0 1.6 13 \~ 6'11.~**(l,_ J 3 .. 3i4.0 sq.a_ 2.2 .. 01 COAN~ "!n ll) 3°J.f) ~l1.z 0.2- 1 -Lo \ t-lP 1Jc ,Jq.i 3~.Y O;z_ (O.t.:o ( ~.l"lh
- 31.-'f 3q,'9- 0 .-z_:
-.,_., Y.O J lbe'v- . ... . _.,...L.1.,,, - "Lil*~ . 1.-1.1 ) Note; If >151111 ortitrant is used; sampf;m11s1 be dilu1cd. Reviewed by: I .-1 r- . Date rev1ew~d I 0'.1- ~*rt l Pa ge 65of93 l)\. SOP C7 - Exhibit C7; l, revision 01-01-13 Sequoyah Nuclear Plant Biomonitoring July 25 -August 01, 2017 AppendixD Reference Toxicant Test and Control Chart Page 66 of93 Ceriodaphnia .dubia Chronic Reference. Toxicant Control Chart , Envlranm'cotal Tcsrlng Solurtcms. lrrc.. Source: ln~house Culture Control Limits (+/- 2 Standard Deviations) 1.08 --~~~~!-~.~-~~~~~~~-.;;.-~o..-~~e~~~~~~-o
- 1.06'
- e " °'
--------------------------4-*
- 1.04 I.
1.15 ... j . . . . . . . . . _..,.,....., t . . . . . . . ' * .,_ . . . . . . . . . . . * * * * * * * ' . . . . . . . . . . . . . . . . . . . . . . . . . 1 . . . . . . . . . . . 11' . . . . . . . . . . . *t-*I.****** * ***** .. , ...... .. Warning Limits .-*._cii_. 1.10 1.05 ---.-***~-*--*-**--*-**-**-***-**-***-**-**-*--**-**-**-**-****-*** --- * ~- e -~ e-*-- . . . _ _ . * - - - * . - * ,., ... - - * - * . - . . .. f * .....,,. . . . . . . - .... - - . . . . ._ . ' *--- .... C'I LOO * - ~* **! ' .. - . . . . . . . . . . . . . . . . . . . . . . . . . . . ~ ........................................... - ........ !'. ~ .............. - - . . . . . . . . . . . . . . . ! . . . . . . - ..... - - .......... ~ ~. - Test date
- 7-.day IC25 = 25% inhibition concentration. An estimation of the sodium chloride concenttatior;i whichwould cause a 25% reduction in Ceriodaphnia reproduction (calculated using ToxCalc).
Central Tendeney (rnean*lqgarithmic IC25 converted to anti-logarithmic values) - - - Con~rol Limits (mean logarithmic IC25 +/- 2 standard deviations.converted to anti-logarithn1ic values) - *. - . L:aboratory Warning Limits (mean logarithmic IC25 +/- 2 coefficent of variations converted to anti-logarithmic values)
- ******** USEPA Warning Limits (mean logarithmic IC25 +/- sA.io converted to anti-logarithmic.values, sA.ia =10 n percentile ofCVs reported nationally by USEPA.l 1
age 67 of93 ~-- i , Ceriodaphnia dubia Q) ~ Chronic Reference Toxkant Control Chart 0)\. -------- co ' fovl11*rnn.,n1.ilT1n.U11!l !it1hJtl1un1fo*
- Source: ln*house Culture
-co 0 Log 10 Conversion Anti-logarithmic Values (g/L NaCl) Test number Test date 7-da"y IC25 Litioratory Ca!cula~ed CV 10th Percentile CV ToxCal Determination 7-day IC25 Cl s CT (.ontrol Limits Warning Limits Warning Limits (g/L NaCl} -*-* , er. :?.S CT+ZS cr~zcv CT+2CV CT - SA,10 CT+ SA.Ia 1 03-08-16 1,0690 0.0,290 0.0281 0.0032 1.0668 1.0510 1.0828 1;0520 1.0815 0.9814 1.1521 2 04-05-16 1.0720 0.0302 0.0279 0.0029 1.0662 1.0521 l.0806 1.0529 1.0795 0;9809 1.15i5 3 04-05-16 1.0627 0.0264 0.0276 0.0028 1.0656 L0520 1.0794 1.0528 1.0784 0,9804 1.1509* 4 os-io~1s 1!0605 0.0255 .0.0275 0.0028 1.0654 1.0516 l.0793 1.05211 1.0783 0.9801 1.1506 s 06*07-16 1.0538 0.0228 0.0270 0.0026 1.0640 1.0512 1.0770 1.0520 1.0760 0.9789 1.1491 5 07-12-16 1.0579 0.0244 0:0268 0.0027 i.0637 1.0507 1.0770 1.0515 1.0760 0.9786 1.1488 7 os:o2-16 . 1.0662 0.0278 0.0268 ci.0027 1.0637 1.0507 1.0768 1.0515 1.0759 0.9786 L1487 8 09-13-16 1.0623 0.0265 0.0269 0.0026 i.0640 l,0515 1.0766 1,0523 1.0757 0.9789 1.1491 9 10-04-16 1.0758 (l.632J 0.0273 0,0028 1.0648 1.0511 1.0786 1.0519 1.0776 0.9796 '1.1499 10 11-01"16 i.0785 0.03?.8 0.0274 0.0030 1.0652 1.0504 1.0803 .1.0.513 1.0792 0,9800 1.1505 1l 12-06-16 l.0753 0.031.5 0,0274 0.0030. 1.0652 1.0505 1.0800 1.0514 1.0789 0.9800 1.1504 1i 01-10-11 1.t\123 0.0303 0.0176 0.0031 1.0656 Hl506 . l.mios 1,0S:l,S 1.07% 0.9803 1.1508 13 Oi-D7*i7 1.0641 o,021q 0.0278 0.0029 1.0660 1.0517 1.0804 1.0526 l.0794 0.9807 1.1513 14 03-07-17 l.0641 o;ono iJ.0276 .0.0029' 1,0657 1.0516 1.0800 1.0524 1.078Q o.~804 1.1509 15 03-07-17 1;0614 0.0259 0.0276 0.0029 1,0656 1.0515 1.0800 1.0~24 1.0789 0.9804 1:1509 16 04-04-11 1.0654 0.0275 0.0274 0;00~8 1.0652 1.0516 1.0791 1.0524 l.Q781 o.~soo 1.1505 l7 05"02.-17 1.0691 0.0290 0.0275 0.0028 1.0654 1.0516 1.0794 1..0525 1.0783 0.9802 1.1506 l8 06,06,17 1,0614 o *.02s9 0.0276 O.ODF 1.0657 1.0524 1.0791 .1.0532 1.07?1 0.9804 1.1509 19 06-06-17 1.0518 0.0219 0.0275 0.0029 1.0653 1.0511 1.0798 1.0520 1.0787 0.9801 1.1506 20 07-11-:17 1.0631 0.0266 0.0275 0.0029 1.0654 1.0512 1.0798 1.0520 1.0787 0.9802 1.1506 {'lore: 7-day IC:M; 25% illhibitlon cor1ceri1ration, An estimation of the sodium chloricle ~oncentratlon !llD! WO\lld i:<iuse a 25% reduCUon ifl C~riodoplmio. reprod11ctio1i (carwlateo u~ing ToxCai(<), CT= Central tendent\' or the IC~ valllei. S = Standard deviation oi th$ IC:;. lla!ues. control limits= Me.:in logarilhflli< re,,+/- 2 standard de*1ialions con1Jerted to anrHogJrithniic values. W;irnlnJl Uini~ =Mean logariihrn*~ 1c,, z 2CV or s:.,1a coo~erred to antl-logarlthrrilc '>'.31ues. SA.io; Sr.inoarti clevjat*tm corcespondlns to tl'\e* 10 11 ' p~rcentlle of CVs repo!Jed na.uonally by USEPA (SA 10 = O.O.B); CV= Co2fflcienl ol variation. Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: ln,.house Culture 40 ! 0 ~-~ --*- --- ---- *--- ~---- <1*-- ~~~---~~-~--=e-~~~--~~~*-~~~o~--0*~-*-~*~~--0 30 ~-~ Q * * * --o- ~-- --- --- --~ --~- --- ---- --- 20 USEPA Minimum Acceptance Criteria (> 15 offspring per surviving female) ~ c0 USEPA Max*1m\,lm Coefficient of Variation Guidance Criteria(" 42%), l\ICDEQ Maximum(< 40%) c +; U. .2 .... ::I IU "O 30
- cRI 0
'Q. ..... w 20 0:: 0 i::: OJ 'u c:0 !EQJ u 0 'O u .....0 10 0 ...__ ____ ~-- -*-*--------=*--=--*~ --** ---- ~--~ _.:..._~- 50 USEPA Upper PMSD Bound.Acceptance Criteria(< 47%) 40 USE PA 30 Lower PMSD Bound (>13%) 20 10 .- -9:: - - *- -..::::..:m.=.::- - -*- -e- -. -.r - -=-=.~ - ** - - - .,. - -* - o - -.- -.- -. 0 ----*::=-.9.- ~~~~~~~~~~~~~~~~~~~~~~~~~~~!~~~~ ---*----------*--O----**~*-*------- __ ..;..,_ *'O *Q .. ro *~ .. (, *O *O *O .(:) -.\:> "co*> ".,.... t\<:i*> IY'" ", ... *V"' ~V"' ... ..,,... Cl.*> "v"..,.,..v .ro *1 *1 ..1 ..1 ..1 ..1* ..1 *1 ....1 r.ro* .. .... ()*"' 01*> 01*"' 01* .. 0 o.*> oV" r::,'O* .. (::,':>'" 'V" t::>"""' ot..*" or.i.*"' l\:Jss o'O"' o1*" o<o* c~*> \.o*0 ..,......v o'V" ()V o,. o,. oo.* o<:i* ()'O* cro* ()"1'\. Test date ii Control Reproduction, Coefficient of Variation (CV) or Percent Minimum Significant Difference (PMSD} PMSD Ts the percent minimum significant difference between the control and treatment that can be declared statistically significant. *rhe lower PMSD bound represents a practical limit to the sensitivity of the test method and is not a minimum acceptance.*criteria. Central Tendency (ml':!an Control Reproduction, CV or PMSD) 95% Confidence Interval (mean*C9ntrol Reproduction. CV or PMSD +/- 2 Stcindard Eleviations} Ceriodaphnia dubia Chronic Reference Toxkant Testing, Test Acceptability Criteria Source: In-house Culture co ToxCal Determination Control Reproduction Control Reproduction CV TestPMSD w Control Control Reproduction Test (offspring/(emaleJ l'i*J (%) Test number Test date Survival Mean CV MSD PMSD CT 95%Corifldence Interval CT 95% Confidence Interval CT .95% Confidence Interval (%) (offspring/female) {%) (%) CT-25 CTt 25 CT-25 CT+2S CT-2S CT+_25 l 03-08-16 100 29.4. 5.4 1;7490 5.9 31.2 28.7 ;33.6 4,7 2,1 7.3 s.a
- 4.3 7.3 2 04-05-16 100 29.3 6.0 1.7920 6.1 31.1 "28.6 33.6 4.8 2.1 7.4 5.7 4.5 7.0 3 04-05-16 100 32.3 4.9 1.6950 5.2 31.2 18.6 33.7 4.S 2.3 7.4 5.7 4,4 6,9
~ 05-10-16 100 31;6 5.0 1.6360 5.2 31.2 2S.8 33.7 4.3 2.3 7A 5.6 ii.LI 6.9 5 06-07-16 100 27;9 2.6 l;731U 6.2 31.0 :rn.3 33.7 4.7 2.ci 7.3 5.6, 4,4 6,9 6 07-12-16 100 32.5 4.9 1.6710 5.1 31.0 28.3 33.'7 4.7- 2..1 7.3 5.6* 4.4 6.9 7 08-02-16 100 *29.!! 6,6 2;1010 7.0 30,S 2S.2 335 4.8 2.2 7.S 5,7 4~ 7.i 8 09-13-16 100 29,7 5.9 2.0410 6.9 30.7 28.2 3B ~.B 2.2 7.5 5.7 4.2 7.2 9 10-04-16 100 28,9 3~4 1:6720 5~8 30.5 28.0 33.1 4.2 2.0 75 5.8 4.3 7.2 *10 11-01-16 100 2s;7 4.4 i.8440 6.4 30.4 17.8 33.0 4.7 2.0 7..5 5.8.' 4.3 7.3 11 12-06*16 100 29.3 5.1 1.4550 5.0 30.4 17.7 33.0 4.8 2.3 7.4 5.8 4.3 7.3 l2 01-10-17 100 30.5 3.2 1;5240 5.0 30.4 27.7 33.Q 4.~ 2,3 7,4 5.8. 4.3 73 13 02-07-17' 100 17.9 8.5 l.8950 6.8 30.2 27.4 33.0 5.1 2.2 s.o 5.9. *11.4' 7.. 4 14 03-07-17 100 29.7 3,9 1.5300 5.2 30.2 2.7.4 33.0 5.1 2.2 8.0 5.? 4.3 7.11 15 03-07-17 100 19.9. 5.3 1.9180 6A 30.2 27.4 33.1 5.. 1 2~2 8.0 5.9 4.3 7.. 4 1,6 04-04-17 100 30.3 4.9 1.9240 6.3 30,2 27.4 32.9 .5.1 2,2 7.9 5,8 4.3 7.3 '17 05-02-17 ll)O 31.0 5.9 1.8940 6.1 30.2 27.4 32.9 5,1 2~3 M 5.8' 1).3 7.3 18 06-06-17 100 30.7 4.9 1.9680 6.11 30.2 27.4 32;9 5.2 2.5 7;9 s.9 tl.6 7.3 19 06*06-17 100 33.1 4.6 2,0500 6.2 30~2 27.3. 33.2 5.1 2.5 7.7 5.9 '1.6 7.3 20 07-11-17 100 31.0 4.8 1.6610 5.4 30.2 27.3' 33.l 5.0 2.5 7.6 5.9 lj.6 7-3 Note* Control Survival= USEPA minirl'lurn test acc~ptabllltvtrneila 2 ail~* >u:viv;;I. C>Jntrol Mean Rep,oducUon = USE PA rnlnimum test acceptability criteri<> '!: 15 0Hsprl11g/sorviv111z fen>c.le. CV= <:oefllcient of variation for to1111 oil eprc11.!utt1or. U5Ef'tl maximum GV ~uidance criteria (9011 ' p~rcenlalej ~ d2%- NCOEQm;i~in1urn CVa"ept~oce criteria~ 40%, MSD = Minimum 'ignificant cliffe1ence, PMSD = Percent minin1um significant tliHe1ente. _ PMSD Is a me'asute of test precision. 1he PMSD 1s the minimum percent difference between the cont10Lano (f~atmem th>t can be declared statistically iig,nliicant ht* who!<? l!!fiuent toxiti!V 1e11. Lower PMSO l>ouno dete,mi'r.ec.I by US(PA'.(10'" pe*cen!ile) "'13%. The lower PMSD uound represents a.practical limit to 1he.~en~illvit.v of th!! test mellmd and b not a mlnlmwn*acc~pt*n.{e.r.ritNia. Upoer PMSD bound acceptllnce trilerln de1ermi11ed by USE~A (90' 1' percentile)< *P'.'f CT= r~mral tendency o! the reproduc11o*n. r:.J or PMSDvalues. S = Slandard oeviat1on.of the reproductJ011, i:v~or PMSD villues. ~ r:: '-r* ,(7:1 SIJdium Chloride Chronk Reference Toxicant Test (EPA~821-R-02-013 Method 1002.0) Species: Ceriorlnplmia dubia CdNaCICR #: 204 Dilut/011 preparation i11formation: Comments: .NaCl Stock INSS number: INSS \\:;ti\ Stoc:k pr~pnmtion: 100 gNnCl/L: Dissolve 50 g NaCl in "500 mL Milli-Q'wnlcr. Dilution prep (mg.IL) 600. 800 1000. 1200 1400 Stock volume (mL) 9 12 "15 1s* 21 Diluent volume (mL) 1491. 1488 .1435 1482 1479 Total volume (rnL) 1500 1500 1500 1500 1500 Test organism source i11(ormatio11: Test i11for111atio11: On?nnisiti:1ge: I < 2<1-hours old Rai1dori1izing lcmrilatc color: ri:e.~ Dale and*limcs org~itisms were bom I (!'\- ,,. , , Olo~tl "TO o'\OS .Incubator omnbcr and shelf between: location: t.J)\ Culture board: OHA.*O A Replicate number.* I ~ :t 3 I ~ I .s I 6 I 1 J s I 9 I 10 YWTbatch: Cullurc board cuo nurnbcr:* Transfer vi:sscl information:
- . '° I l I\\ I\~ Ii\... I\'\ IU..11..~ l"b\
pH= & * \ "LS.U. Temperature= 'l,.J,.'\ °C Sele11osm1m batch: a\ *ti.*\'"\ O'\*~-li Average transfer volume (n1L}: <0.25 mL Dni~p *renewal iilformatiim: Dny Dnte 'Test 'initintion *and feeding, MHSW t\ nalyst . renewal :111d feeding, or l>atcli used termination time 0 07-11-17 Ci'\ iS O"\~ or.:.q x\ 1 07-12-17 ('}~t.ls IS\. Q I.-\"\ ~l 2 07-13-17 o~~ -z... en-~ "l"""\ ii J 07-14-17 ot'-\~ . trhtr\'1 :;( 4 07-15-17 ()'\_ \~ crt* n..*n -j{ .5 07-IG-l7 6C\\~ .6'\*\!,* \-\ . ~f 6 07-17-17 ogl\4 tr\.,.\'9* \~.. ..... -.~** r 11 ~.\ 7 0'7-1$-17 C>&\S --*- .. . *- ' ' Control i11fonuatitm: ~cccpt3ncc cfitcri.a Sm1inu11*11 of test endpoints: %.ofMaleAdults: 01. 520% 7-day LCso ") ILlOO % Adults having 3r<1 Broods; ICTJ1. ?.:80% NOEC 1000 % Mortality: n1. QO% LOEC n.oo M~an Offspring/Female: ~\. Q *?.: 15.0 offsprii1g/fcnrnlc ChV ll11S.S %CV: ~.&7. < 40.0 'Yo IC25 loL:.'D.\ age 71 of93 SOP AT14-Exhibit AT.14.I, revision 11-01-lil Page 2-of6 Species: C1triotlaplmia dubia CdNaCICR #: 204 CONTROL.
- Sw*vivatalul Re111*of{rfctioll Dara Replicate number O:w 1 2 3 4 5 6 1 8 9 10 I 'i'oung pratlncc:cJ 0 0 D 0 0 0 6 () 0 n At!uh mortality L '- L. L \_. L \.._ L_ '-- '--
i Young produced ' {'\ C". ('\ ('\ ~ 6 D () a 0 Atlu!r rnort:ilicy "-- L- \.... \....._. L '- '-- L '--* \,._, ..3 Young produced () a D () 0 0 0 0 o* a Atlull rncirtaliry L- '-- \._. \:....- L-.. '- \..._ '- "L- L.. ~ Young produced '-\ \.\ ~ ~ 5 Y. c...\ '5 "'\ *'-\ t\cJull mortalily L. \.._ L. ~ ' -.. "- - '\...., \__ L. 5 \'oung (lrotluccd H v~ \~ ,,_ tb \ "'L; \.b \ \.. \""' I,...._ Adult 111or1nlity L L L \,,,.... L '-- \._ '- L \..._ 6 Vomig produced 0 0 0 (')' a ~ L... 0 c 0 {) Adult mortallt)** \..._. L \_ L. '- \..... '-.,. '-- \....... 7 Young produced \.~ \~ \\ \ '-\. \~ \ '--\ \\: \S \'-\ l \.-,. Tol:il you11g.pr1,1!luced ~\ ~D ~tl ~~ *~ ~t) 3t> ¢~ ~1.. ~o l'illnl Adult i\'lortnlity L \._ \;_,_ '- \..... '-- L_. '- ' "-- '-- X for 3rd Broods \'.'" "'£_ .><. .><. )<. ')<._ 1'-- f<.- J<-, 7-*. Nn1c: Adult monulilY (L =five, D = dc:ml). SO =split bniotJ.(singlc: brood split between nvo dnys), CO= Clll'IJ over (offspring cnm~d over wi1l1 ntlult dul'ing transfer). Cimcentmtio11: 'Yo Mortality: I 07. Mean Offspring/Female: I 11\t.o 600 mg NaCl/L SurJJiva/ <11ul Repro<l11cti011 Data Rcnlicarc 1111rn1Jcr Day. 2 .'.\ 5 G s !) HI I \'onllg 1irod11cccJ f) J () f\ "0 0' 0 7 0 n 0 0 \,_. "'-- \.:_ L .L Atlull morl:ility L L- '- '--- L 2 Voung produced 0 0 a 0 l) D 0 0 0 'O 3 Adull mortality Young p1*oduccd ' -n '- {) () \._. () () L (') L C) 0 L () \...... 0 Aduft,mortality L L \._ L. \._ \,__ L '- \._ '- *I \'oung ptoducccJ \.. '-\ '-\ ~\. s :1-,. ~ \.._ "-\. ~\ '..\. Adult mortality -\_. \,_ L.. \....... \-... '- \.._ \....:.. \..._
- , Young produced Adult mortalit)*
l '.\_. L. \b L \~ *to L 11.. l~ \.;._. 10 \._ I °?-i \.._, \~ '- - {"\ (j Young procluccd I"'\ 0 {) 0 {\ 0 L a 0 a a* L-rn~rinlity \._ L L L Aduh '- L L 'L \'otuig produced l'Q n. .11 l~ l'l., t'S *~ 7* l\a 11... { Total young, produced °'?) \ ~~ ~'-\ ""L.~ :,u. ~\ ~\ ~~ &l '1..'\ Fin:il Adult Mortality '- "-- \.....: L. \.._ L. '- L -.. ' - '- Note: Adult mortaliLy (L =live. O =dead). SB =split broad (single brood.split bctm;cr\ !WO days), CO= carry over (offapring 1;orricd avc1.wi1h odult il1.11fog tronsfcr).
- Co11ceJ1tratiou:
% Mo11ality: 01. Mean Offspring/Female: ,";1,!, \a % Reduction from Control: .. ,,q7_ SOP AT14 -Exhibit ATJ4. I, revisio_n* 11-<H-l.tl ~ p !.. :..l /;:::J I, PageJ of6 'l .'htl'l-o*u:t-.nlJlfdlfJ'll}W...:.....i.~
- l Species: Ceriodaplwitt 1ilibia CdNaCICl~ fl: 21M 800 mg NaCIJL Survival and Repl'0tl11ction Data Replicate number Day I 2 j 4 5 6 7 8 9 IO
*1 Young produced () 0 a a D () c 0 0 0 Aclnlt mo1*tnlit)' '-- '- \,_ \_ \..._ L '- L L. l_" 2 Young produced b 0 CJ. D 0 a 0 *a 0 (J Adult lllortalily '- \.. L L- \__ L "-- "--- \...... J \'oung:produccd {') 0 0 (<) () 0 0 0 0 0 L Aclult mortality \.._ '- L "\._ '- '- \...... L- L.... 4 Young produced \, *'-\ "'\ s : '4.. s ~ '-\,___ ~ ~ Adult mortality * '- \..... L \._.. - L L- \_, '\.._ \ 1-5 Young produced *Adult mortality \ l \.._ \.~ \""" .l \ \ 1... '-- \_.. ..~ ~ l <:::.i \~ \0 x__ 6 Young proclucctl D r) (') lJ a c n n () 0 Adult morrnlity '- *'-- (_ L L '- \..._.' \._ \...__ I...... 7 Young produced l I,,,. \ '-\ \ \c, '~ *S I'?=> 1to \ '-\ 1'-\* \S To~:il young produc~d ':>~ "'!> \ :,'!> ~'l.. ~\ ~~ ~~ 1.& !>\ -==o Fih:ll Arl1ill Morlnlity \_. \....... '- '- \...... L '- L. I.... \._ Nole: Adult mortality (L =live, D =cl end). SB= split bro~>tl {sing.le.brood split between two clays), CO= carry over {offspring CilJTic.d*ov~r wilh odolt.r.Juring*lmnsfcr);
- Co11ce11trnlion:
% Mmtality: 07. *Mean Offspring/Female: ~\. "l- % Reduction from Control: -o.i. 7. 1000 mg NaCl/L Sm*i*ival mu/ Reproductio1z Data Rc111ic:1lc"11umbcr Ony 1 2 3. s 6 9 " 7 8 IQ \'ouug ptoduccd 0 0 0 0 () I () 0 0 0 0 Adult morlnfity \...._ L l- L L L.'. '-- L L L l Young produccll (") 0 0 0 0 0 0 0 0 0 Adult mortnl.ity \_.. '-- '- '--- '- 1-. \.._ '- \._.. \_ il Young produced Adult niorlnlily D L t\ L. D \._. a \._.. 0 0 \..- (') '\.._ 0 L L 0 Ci L 4 \'oung produced "-\ 9 lo . -~ s $ '-l c...\ I '-\ ~ /\d1il1 ruorlality '-* '- '- \._ '- L \._ '- L 5 .Young produced Ib ,,,_; 1.\ \\ t'"L 1() t-z_ \t_ \._ \.C J "L Adult mrmnlily "'-- '- L. '\,..... '- '- \._ L L '- t') . () .0 _\'oung produced D {) 0 0 0 0 0 " Adult mortality l- L L L L "L L- L L L-7 Young.prod need ~ I '1 J~ \ '5 \"~ \~ \b l '4 IS 12. Total yom1g produi:cd -i.,g.* "'1.""\ ~~ ~<:) ~ "& 1. l..C\ ~() 1..~ "'~ 'Final Aduli i\"lorlnlity \..... I L L \._ '- \._ L \ ,Vq1e: Adult mo11olity (L = live.. D = dcnd), SB= split brood (single- broad split between 1wo days); CO= carry ovcr (oflsprmg carried over wiU1 adult during transfer) .. Conceniratiou: % Mortality: 01. Mean Offspt;ing/Female: "2frt .(:, % Rt9uction from Control: .:.. sl. SOP AT14 -Exhibit ATl4. I, revisi.on l 1-0 l-14 age 73 of93 ~ ..fl.:.;_-.::.- ...~ Page 4 of6 -7: : --- . ~~,.. I Species: Ceriodaplmia rfubia CdNaCICR #: 20tl l 1200 mg NaCl/L Survival mu/ Reproduction Dula .RepUc:.te nu miler Dar I. .2 3 4 5 6 7 8 9 l (I 1 Young produced () . *() ,_ 0 0 0 0 0 ..__ 0 0 0 Adult 111orfnlity \_. '- \.._ '- L L '- '-- '2 \'oung produced ("\ *O C) D D .._ ' 0 L-. 6 a 0 () Adult 11111rtalit)" '- L.- '- \._ '- '- \..-' .......... J Voung produced 6 D ('} {') 0 0 () D r) {J \_. . \;_.,.. \..;.....: Adult mort:ilit~ \..__ '-- .,..._ '-- \..,;;.. \.__.. '-- '-- J Young produced '3i ~ '-\. ""L 5 ~ ?:>>. *"-\ Adult*mo1<<nlit)I \,_ \..._' \_ *~ '-- '-- '-- '-- '- '-- Young procfuc~d 'S. ::::a ta q 5 Adult mortnlif)' . \_ \..._ L. "1... \..._ '-\. '- \- \ ~ \...... ' \..... 5 \: (i Young produced D n i (:) /j 0 a c .L 0 () a 7 1\dul! JllOl'l:llily Young proLluccd 4. ,_ L. I_. '-- '- \_ S. ~ L L. '.\ ~ l .Total young produc~d \~ "' JO \\ 8 '\ ' ~ q .\ ~\ '1- l\ . Final Adult iHort:llity \_. '- L: '\;_. ~ \.- '-' '- L. L Note: Adult n1orti'llily (L =five, D =-dead), SB =split brood (single. brood split bctwcr.u two days), CO c <.:arry over* (offspring carried over with nl1!11l-d11ring trnnsft:r). *
- Co1tcl!ntrafioll:
%Mortality: 07. Mean Offspring/Female: lo. "l- % Reduction from Control: eo1.1 7. 1400 mgNaCI/L Sim,flral amt Ri!pi:oductio11 Data Rcolic:ilc number DR\' [ 2 J 4 5 6. 7 s 9 HJ I Young produced 0 0 n* 0 0 0 0 0 C> 0 Ailult m1Jrtnlity '- L L L L \._ '- '- L. L 2 Young. produced tr"'. c [\ {\ a 0 L b 0 0 \._ 0 Adult morlality \_ '- '- '- L \..... \_ L J Young produced CJ *o a 0 £') 0 () (") n {') \._. \..... L L L \;,,. '-- '- .\...... J Adul1.mor1ality Young produced* \ 1-. .,_ :'b \ ~ \ \ \. \ Adult*morrnlity \_. \.._ L \._ \..._ '-- '- \..._ L \..__ 5 Young produced 2.. 0 \ D "'2.- : 0 '- 0 0 0 Adult m*lrCO\llt~* L '-- '\_ \.._. \_ '- L. '- \_.. G Young produced a \ 0 ?::i 0 \ L. 0 1... 0 1\dult mortalily- L L \..... L '-- \_.. L L L 7 Young produced l 0 0 0 '- .. 0 () C\ 0 \ Total young prollm:cd ~ ~ b (,, s ~ ~ ::,. ~ 2-. Final Adult Morini it)' L '- '-- L ~ L '- '--- '- '--- Note; Adult mortality (L =live, D = dc::id), SIJ =split brood (single brood ~plit bctwccn 1wo days), CO =.carry over (oflspnng - cnrricd O\'Cr with nduft during trnnster), *
- Conce11tratio11:
'%Mortality: Ot Mean Offapring/Fe111ale: ::l..~ /r~ ,.,,,...,,,. \ , % ReducLibri from,ControE &~. '1 7. .....~:: ..*.Page *74 of 93 SOP ATl4- Exhibit ATl4. L, revision 11~01-14 / i ,_. --' f,___1 I I Q) CQ CD I Verlficatio11 of Cerladaphi1/a Reproduction Totals ~ \ EnvlronnientalTe.sttng Solu~lons; Inc. 0 co C(lntrol 1000 mg NaCl/L (<\) Replicate number. Replicate number Day Total Day Total 1 l 3 4 5 6 7 B 9 10 1 2 3 ' 4* 5 6 7 8 9 10 1 0 0 0 *o 0 .0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0, 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 *o 0 0 3 Q. 0 0 0 .o 0 0 0 0 0 0 *3 0 tl 0 0 0 0. 0 0 0 0 0 4 4 4 3 *~ 5 4 4 s ,4 4 41 A 4 4 G 4 5 5 4 4 4 A 44 5 li 13 . 13 ' 12~ 10 12 13 .12 13 10 119 s .10 12. . 11 11 12* 10 :12 . 12 10 i2 112. - 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0. 0 0 0 0 7 15 13 17 -14 15 *14 17 15 14 16 150 7 13 . 17. . 13 15 15' 13 13 . . 14 15. 12 140 Total . 30. 30. 33 30 30 30 34 .32 31 30 310 Total 27. 33 30 30* 32 28 29* .-30 "29 28 296 GOO.mg NaCl/l 1200 mg NaCl/l Replicate* number Rep!fcate riumber Day 'Total ciay 1 :i. .3 10 Total 1 2 3 4 5 6 7 8 9 10 4 .5 6 7 8 9 1 0 0 ,0 0 0 0 0 0 0 0 0 1 0 0 0 Q 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 'O .0 0 0, 0 Q, 3 *o 0 0 0 0 0 0 .0 . *a o* 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 . 4. ~ s 3 s *4 .t\ l\3 t 3'. 3 2* ' 3 3. . 3* .. .. 4 31 5 11 . 13"' 4 13" 10 12 13 10 13- B 4 10* 119 "5 s 3 6 4 2 4 '4' *_ 1 3 1* 5 4 40 6 0 0 0 0 0 0 0 0 0 0 0 6 *O -: . *o 0 0 0 .o 0 0 0 0 0 7 13 16 17 15 17 15 15 16 lll 15 1511 7 6 4 2 2 l 5 3 1 4 3 31 Total 31 33* 34 .29 34 31 . 31 33 31 29 316 Total 13 10 I 11. ,g 9. s 9 11 *12 11
- 1(}2 ,
8QO nig NaCl/l 1400 mg NaCl/L lteplicate nu'mber lteplicate number Da*1 Total Day Total l 2. 3 4 5 6 7 8 9 10 1 z .3 4 5 6 7 '8 9 10 l 0 0 0 *o 0 0 0 0 0. 0 0 .1 0 0 0 0 0 0 0 0 0 0 0 .2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 .. 0 .. 0 0 0 0. 3 0 0 0 0 .o a 0 a .0 . 0 a .4 6 4 4 ". 5 4 5 4 4 4 5 45 4 1 2 2 3 l 2 1 1 1 1 15
- 5. 11- 13 n 11 12 12 13 10 13 10 118 s 2 o* 1 0 2 0 ' 2 0 0 0 7 6 0 0 0 .o 0 0 0 (j 0 0 0 6 0 1 0 3 0 .1 0 2 *2 0 !l 7 16 H 16. 16. 15 13 16 1*1 14 15 149 7 1 0 0 0 2 0 0 0 0 1 4
'Total .. 93 31 3.; 32 31' 30 33 .28 31 30 312 Total 4 3 3 G 5 3 3 3 ~ .2 35 /!-_~*....... ,.¥! _",.-~~ ,..~t;,~ Ceriodciplmia d11blC1 Chronic Reference Toxicant.Test EPA*821*R*02~013, Method 1002.0 Environmental Testing SoJutrons. Jnc-. Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: _ _ _ _ _ _ _ _Cd-'._N_a_C_lC_R_<<_2_o_q Test dates: ,uly il-18, 2017 C911centratlon Replicate number Sur11ival ** Average reproduction *Coefficient of Percent reduction (rng/L NaCl) i 2. 3 4 5 6 7 8 9 10 (%) (offspring/female] 11ariation* (%) from control (%) Control 30 30 33 30 30 30 3~ 32 31 30 100 31.0 4.S Not applicable 600 31 3:! 3<1 29 34 31 3i 33 31 29 100 :ll.6 5.8 -1.9 800 33 31 33 32 31 30 33 28 31 30 100 31.Z *s,z -0,6_ 1000 27 33 ~o 30 32 28 29 30 29 28 100 29.6 6.2 ol.5 1200 B 10 11 8 9 8 9. i1 i2 li 100 10.i 16.S 67.l ~400 *4 3 3 6 5 3 3 3 :i* 2 100 3.5 33,7 l!S.7 Dunnett's MSD value: *1.661 MSD= Minimum Significant Difference PMSD: PMSD= Percent Minimum Significant Difference PMSD is a measure of ti:st precision. The PMSD Is lhe minimun1 percent difference hetween the control and treatment that can ba declared statistically significant.in a whole emu~nt toxicity testi 11 Lower PMSD bound determined by USE PA (10 ' percentile) = 13%: Upper PMSD bound rlelerm\neil by \JSEPA \90' 1' percentile)= 47'1'., Lower ancl upper PMSD bounds were determined from the 10th and 90th percenlile, *espectivel\*, of PMSD tlata from EPA'~ WETlnterlahoratory Variability Study (l.JSEPA, 200la; US EPA, 200lb). The lower PMSO bound represents a practical limit fo the sensitivitv*ol the iest niethod and is not~ mlnimumcacceotance criteria. l)SEPA. 200la, 200lb. Final .Report: lnterlaboratory Yariabilitv Studv of EPA Shorr-ierm Chronic and Acute Whole Efthmnl To~icity.Tes\ Melh[)ds, \lolome~ 1 and 2-App.,ndix. EP;\,82l-B'01-00tl and EPA*B2J -!!*Dl-005. US Envh'ClnmentaJ Protettlon A11e11cy, Cincinnati; IJH. .$tatistltal J'\nalyses *Cerlodaphnia*Sucvlval:and:Repl'OductfonTest*Repto>ductiori *Start,Date: 111112017 Tesl*I[): CdNaCICR *Sampffi: ID; *RE:F:Ref Toxlcar,t End. oaie1 711~12017* Lab" 10: EiS'EnVir. Testing Sol, .sample* Type;. NACL-Sodlum *ctlloride sample*Oat.e: *Pro.tD.c>.:>1: FWCHR*EPA:~21-R.02*01:l Twt.*Spe~lss:. co-eaii~pnnia cub~
- Comments:
.Conc-mg/L 1 .2. .. 3 4- .s. .6 7 a* 9 10 D'COnfroL* 30.000 30,0.00 33.,000' 30;000 30:000.* 3p,ooo ;34:00.0 3.2.000 .31.000 30:000 GOO *:;;11,000 ~3.()00, 34,000 2~;000 34,000* 3i;cioo :31~060 ss*,ooo :fa.coo 29;000 .wo 33:000 31.0.00 3;3,000 .32:cioo 31.000 M.oo_o. 33iOOO .2e:ooo *-ai:.ooo 36,ooo 1000 "27.000 33,0QO 30:.0.0.0 30:000 32:Mei: 28.000 29.000 30.oO:o. :29:000: :za.ooo '1200; 13.000 10,000* .11.006 -8,000 '?.coo i;!.000 9:000 11.000* i2*ooti 11,000' -1400 <'tooo* 3.000. 3.000 e~ooci *S;OOO ;3.ooo. 3;000 S:OOI) 3:000 ?..0()0 Transfol:Tll: Untransformed* *i:tan~* .1-Taifed :1sotprilc
- conc-m9/L_ Mean .N*Mean *Mean *Min: Ma>: *c\1% N Si.im. Critical :Mean N~Mean o~conttol *31.000 1.0000 31.000 :30:.000 . 34.000* 4.809* 10 3*1..300 1.0000
*600.' *31,~oo :i,Ol!;i4 31,600 ~9,ooo* 3{000 '5;816 'Jo 117,00'. 75;oo 31.300 1*000,U *s_oo 31,200 7,0065 :3~.200 .28.000 33.000, '5:190 *to 113.50 75,00 31.200 0;9968 iOQO 29,6,00 *0:9548 29:600 27:.ooo. 33:000 6.2oe 1l1 e.o.oo* 75.00 29.600* 0.9457 *12.00* 10.200 0.3290 10,200 . a.coo 13.000 16.5~5 10 55;00' 75,00 10.200* 0;3259 *11100* .3.SOO *o:.1i29 ,3.~0<i 20_00 6.000 ~3:672 10: ss.Oo is.co 3.500 o.111a Auidliary*TestS Statistic Critical Skew Kurt. Kolmogorov 01Tes1*1~1~1es non-normal.dis_tribution*(p <:= O.o1) . ,,12066. *1,036 Bartlett's Test* indicates egual variances '!p = 0:83\* 2.1681.1 15:0863 H111>olhesis*Tcst{1*tail,*0.05) NOEC: L:OEC' ChV Ttl $teel's'Mariy-Qrie*Rank Te!!I 1ooo 1200 ,1095:45 *Treatments.lis*D-Control.
- Wnear*ln!eri>ola~lo!l.{!lDO'ResamP.les) *
.Point mg/L SO 96o/o'CL * - Skew. . * * !COS* .963;125 58,787<! 642:75 1009.57 *4~1160 IC.10 tOH.74 5,70623 *1062i69 1024,79 *O.Op6i' tc1s. 1030.8_6 5.24817 102CM'i1 . *1040* *D,0731 'hO-.-------------. *tC:io 1047,01 4,83636 1037.73 1055.42- *0.0806 ().9 IC2!! 1oe3. -14 4_4a3ss 1op4.39 1on:02 -o.oeao *o.a* 'IC40 1111.ss *'3.92798 M04.1 111~.'22. -0:oe10 ICSO 114S:e1 4.01644 11.::16,04 11s1 . ai -0.0::13s "" '0,$
- g *o ,...
.o *" .,.~ *o-4 . a:: o.~. D.Z 0.1 ll (I 6--;...;...,-:-,,.-=c,.,..,,-(r'- *ll* ~ -t-.-.-,.-.--.-.,-,.-.-..-.--.,,-...-,....,.-1 a .$qO .1000 Dose'mg/I;.. Oose,Resp,onse Plot 3ri.
- io.b---j---~-~-1
;g.. :25 :. ~ 2 20, Cl. ~* 1~. 10' s' o.~~~.----cr----..,-----..-----.------1g. l* 0 ~ ~ Cl age 77 of93 Statistical Analyses Used for PMSD calculation only. Cer!odaplinla Survival and Reproduction 1est-Reproduction Start Dale: 7/1112017 Test ID: CdNaCICR sample 10: REF-RefTO)<icam End Date: 7/1812017 Lab ID: ETS*EnVir. T~sting Sol. Sample Type: NACL-Sodium ct1lorlde sample Date: Protocol; Fl/VCHR,-EPA-821-R-OZ-013 TestSpei::ies: CD-Cerlodaphnta dUbia Comments: Canc-mg/L 1. 2_ 3 4 5 6 7 ti 9 10 D-Conlrol '30.000 30.000 33.000 30.000 30:000. 30.000 . 34.000 32.000 31.000 30.000 600 31.000 33.000 14:000 29.000 34~000 31.000 31 .000 33.000 31,000 29,000 600 33.000 31.000 33.000 32.000 31.000 "30.000 33.000 28.000 31.0QO 30.000 1000 27.000 33.000 30.000 30.000 321000 2~1000 29 000 30,000* 29.000 28.000 1200 *t3.000 mooo *11.000 8000 9.000 a.boo 9.000' ~ 1.000 14.000' 11.000 1400 4.000 3.000 3:000 6 ..000 5 . 000 3.000 3.000 3.000 3.000 2.000 Transform: .Untransformed 1-Taile.d Conc-mg/L Mean N-Mcan Mean Min Max CV% N t-Stat Critical MSD D-Controt 3*1.000 1,0000 31.000 30.000 '34.000 4.809 rn 600 31.600 1.0104 '31.600 29.000 34.000 5;811] 10 **0.826 2.287 1.1361 800 31 ~200 1.0065 31.200 28.000 33.000 :i.WO 10 -0.275 2,2$7 '1.661 iOOO W.GDO 0.9543 2&.600 27.000 33.000 6.209 10 1.927 2.2.:ri* L6fr1 *1200 i0.200 0,3290 10200 8000 13.000 16.5:35 10 28.631 2.287 1,66'1 "'1400 3.500 Q,1129 3.500 2.000 6.000 33.672 10 37.(~54 2,287 1.66! Auxiliary Tests Statistic Critical Skow Kurt Kolmogorov D Test indicates non-normal distribution (P <= 0.01) 1.12066 1.035 0.25953 -0.5513 Banlett's Test indicates equal variances (p = 0.83/ 2.16811 15.0863 Hypothesis Test (1-tall. 0.05) NOEC LOEC CtiV TU IVISDu MS Op MSB .MSE F-Prob df Dunnelt~s Test -iooo *1200 1095.4~ 1;6!'122 q,05359 *f5$5A3 2-63889 O.OE+OO* 5,54 Treatments vs D-Conlrol ~.:'~**;* l'ihlP"o<rtlf* t',.ll*v t.r~'"'"' V'-"*
- age 78 of 93
,"FE"'"'=lq* .. ,,1--1* 1'.*-:- Page 5 of6 ' ~I I I~ ' - ~ - ) &."'-im":,,~S-:.:~11:1..._ Species: Cel'fodavlmifl .rlubia CdNaCICR #: 2iJ:J J)aif:~r Cltemist1*y: Day (Analyst idemilicd for each day, performed pH, D.O. and conductivily*mcasurcmcnls onl1*;) .o 1 2 -~ - *~1 :~----~ Co11ce1itl'ation Param~tcr ---*~ - __ ; ~. -.r pH (S,U.) DO (mg/L) 1-.e*-- Conductivity . **~*---:,_;_--:-ro* i * (:;-_ * ( J1mhosfcni) 1_ ~,'!;...:_._ .~*.::.~ I .,_ * - " ~* -* - ....... .:.!. CONTROL "Alkalinity I' .-- -- '. f . c_ - . --- ---1 (Ill~ CaC01/L) ~\ ' L__ ~~. ,~' ' 'I. . :1 4 ltarditcss ,.-. --, ' ~ (mg CaCO;IL} *Tcmpcraturc*(0 C} ~&\ *.£ (S.\ U\.~ pH (S;U;) iaiJi- 1.0in 800 . DO (mg/L) r.t.e - .* - >;---, Condncti\'ity .(Jtmhosfcm). .. l'-i?>O *:. - ~* \\.\'ZO *Temperature (0 C) -,,~.<\ -pH (S.U.) 0.IJ\.a DO {mg/L) !\.8 r---.::::-:- . BOO nlg NaCl/L
- Couducii\*iry -~*-: --*1 J11mhos/cm) 11<1o J -- __ :.li <~C:~., '~
~Tcmpernture (°C) "'\..'\.~'\ pll (S,U.) re.oro DO (mg}L) q__g -=1-8 0.0 1000 mg*NaCJ/L Cond11cti11ity P'". *-- . . . . <:;- . . *':i (J*mhos/cm) _r,\ I lPD 7190 !. --*-* , 1 l**~-!~L,.. .:___ ,:1 *Tcmpcr:itlirc ("C). 1..'\-4. ~\.'\ pH (S.U.) e.os-DO (mg/L} '+.<3 e.o 1200 mg NaCl/L Conducti\'ity fl*~-->"\.~?~- -* . I
- (Jtmhos/cm) ~S1-o L .......:1.:~.:... .. "-"*
. *;.Tcmpemturc (°C) "L'\.~ -l,S.D pH (S.U.) O.a5 DO{mg/L). "\-.°> r--* e.o e.o 1400 mg NaCl/L Conductivity -*~. I - - i, ** I (1-11111tos/c111) - AA60 l." ,c, - ~ f. .,. - ;_ ___ ":_,,._,I. *Tcmpcrnturc (°C) [..q,'i Initial Fii1al Initial Final Initial Final ~Tcmpcrnum:s performed at 1l1c time o[ICSl initiation,_rcncwal ortcrminntion by the :m;ilyst identified in lhc Daily Renewal lnforma1io1i mble locntcd on Page J. Allrnliniiy nnd hardness pcrfonned by the an:ilyst idcr}lilicd 01\ tha bench shccl'Spcc'lfic for each nrmlysis.and lmnscribctl lo this bench sheer by: & SOP ATl4-Exhibit AT14.I, revision 11-01-14 age 79of93 Page 6 nf6 I Species: Ceri0da11lmit1 dubia . CdNilCICR ii: 20'1 ~j ,_~--------------~------"T'""----------~-------------------0-ay----~--------~----------------- (Alialyst identified for cacl1,day. perromicd pH, D.O. anll conductivity measurements only.) pH (S.U.) DO (mg/L) Conclucti\'ity - - (~m1hos/i:m) J f._\(-~.J r:"" ..- -= I CONTROL "illk:rlirtity (mg CaCOjfLJ '"flnrclncss I 1., __ , - -... '_.,, (nm CaC03/L) l "Temperature ("C) pl-I (S.U.) DO (mg/L) I 61)0 mg NaCl/L C*rnductivily (1m1hos/c111) ~Temperature
- i. " - . J Y-f40 ., - '
1'52-0 \460 ("C) I pH (S.U;) DO (mg.IL) 800 mg NaCl/L Con cl uctivify (11mboslcm) l~O *'Tempera tun? (oq pH (S.U.} 13.0S DO (mg/L) ,.,-.--: *-**** f) lOOO mg NaCl/L Cor1d11ctivily , (Hmhoslcm) L~~-;*:*-~,::~t~~-;~ "Tempcrat11rc ("C) L.S. \ pH (S.U.J DO (mg/L) ...__-.. ~- I :mo mg NaCl/L Co11ducrivits ' 7. * (unihos/cm) ! 'l *Tempera tu re (VC) \....\.1 pH (S.IJ.) . 'i),O I DO (mg/L) .. -* ** I. 1400 mg NaCl/L Co11duc1Mty .l "c1unhoslcm) Temperature ("C) '1.S .c , 3no Initial Final. Initial Final Initial Finni luitinl Final *Tcmpcrntures performed at the time: oftest initiation, renewal or termination by the analyst identified in the Dnily R.znewal lnformnlion table located 011 Page I. Alkalinity and lim'dncss performed by the annlysl identified on the bench sheet specific: for each annlysis and lrnnscribed 10 this llcnch sheet by: SOPATl4-ExhibilATl4.l, revision l l*Ol-L4 age 80of93 ~ Pimephales promelas Chronic Reference Toxicant Control Chart ( -.,,. EnYttonnumtnl.T~ittng SOfuClon~. tl'1t'- Source: In-house Culture J Control Limits (+/- 2 Standard Deviations) 1,0 '°'--*---- _,_...,.. - --.. . . . . . . __ --*--F'°'-* -.--,,----. __ _ 0.8 0 o
- e e
--*-----~------:"'."'~-----*---.-~ "' * *--~-----8~ -u *o.6 '-
- _____ ., .
- - - -- - --* - -- _ . . ,,,_1 - - 4'-""". _.-------~*-* Cl 0 ~ ...I 0:4 ~ -u Ill N 1.2 - Warning Limits 1.0 ......................... " ............................. .............. - ~ _,, ~
- O e'" * '"' - * <I ' o .-. .._
- o e 1 " '< J " 1" " * * "' '"
- I o * * .. o * * * '" 4 o
- t I I '"- * ., "- *
............. 1 ... _
- I - - *"' - * * - ~
- _ , __ 1' - .. # - * * .._ * * -
- o - * * * - *
- _.._ ~ * - . . - . - - . . o'
-~--. 0.8 () Cl
- ___..~~~~~~~~~~~,;:._~~~~-
e 0.6 e ** _,.,. .. - ".~ .. '°-.. I* -
- o _ . . _ ,._"'"
.............. 4-* ** " ....................................... ' ............... ,. ................ - 0.4 Test date
- 7-day I~ =.25.% inhibition .concentratio*n. An estimation of the potassil1m chloride concentration which woulo cause a 25% reduction in Pimepholes growth (calculated using ToxCalc).
Central Tendency (mean logarithmic IC25 converted to anti-logarithmic values) - ~- Control Limits (mean logarithmic l[,_5 +/- 2 standard deviations converted to anti-logarithmic values) - .. -- . Laboratory Warning Limits (mean logarithmic IC, 5 +/- 2 coeffjcent of variations converted to antHogarithmic values)
- ** * . * * *
- USEPA Warning limits (mean logarithmic IC25 +/- sA. 75 converted to anti-logarithmic values, sA.'7s =75th percentile of CVs reported nationally bv USEPA) age 81 of93
Pil'nephales promelos Chronic Reference Toxicant Control Chart Source: In-house Culture: v ...... log10 Conversion Anti-logarithmic Values,(g/L ICCI) co Test date Laboratory Calculated CV 7Sth Percentile cv Tlfanumber 7-day ICzs ToxCal Determination 7-day IC;s CT s CT Control Limits Warning Limits Warning Limits (g/l KCI) CT-2S CTi-25 CT- 2.t:V crricv cr-sA.75 CT+ st,.7s 1 05-17*16 0.61109 *0.1932 -0;1573 0.0496 0.6961 0.5539 0.8749 0;4918 0.9530 0.4316 0.9569 2 06-07-16 D,6998 -0.1550 -0.1571 .0.0468 0.6965 0.5614 0.8640 0.5026 0.9370 0.4318 0.9674 3 06"14~16 0.7412 -0.1301 -0.1546 0.0451 0.7004 0.5690 0.8623 0.51-27 O.!l3i5 0.4343 0.9729 il 07-12-16 0.7.965 .0;0988 -0.1500 0.0460 0.7080 6.5729 0.8749 o.~172 o.9437 0.4389 0.9834 5 !>7-19-16 0.6348 -0.19711 -0.1536 0.0459 0.7.021 0.5682 0.8674 0..51111 0.9376 0.4353 0:9752 6 08-02-16 0.7850 -0.1051 -0.1502 0.0460 0.7077 0.5726 0.8746 0.5168 0.9436 0.4388 0.9830 7 08-09-16 0.6703 '0.1737 -0.1517 0.04117 o.70Sl 0.5739 0.8664 Q.5190 0.9339 0.4372 0.97911 3 09-12-16 0,7241 *0.1402 -0.1510 0.0433 0.7063 0.5786 0.8622 0.5255 0.9270 0.4379 0.9810 9 09-1i~16 0.7351 *0.1336 -0.1500 0.01121 . 0.7080 0.5831 0.8596 0.5315 0.9222 0.4389 0.9834 10 10-04-16 0.7791 *0.1034 -0.-1477 0.0420'. 0.1111 0.5864 0.8638 0.5357 0,9254 0:4413 0.9886 11 l,l-01-16 0.7808. -0.1074 -0.1456 0.0419 0.71!?"2 0.5897 0.8674 0.5398 , o.92so 0.4434 0;9934 12 12-06"16 0,8303 -0.0808 -0.1423 0.0433 0.7206 0.5904 0.8795 0.5399 0.9411 0;4468 1.0009 n 01-10-17 0.7066 *0.1508 -0.1433 0.0432 0.7189 0.5892 0.8773 0.5384 0.9392 0.4457 0.992.1 14 02.-Ci7-li' 0.705? *0.1517 -0.142;1 0,0426 0.7209 0.5924 0.8772 0.51126 0:9378 0.4469 0.99118 15 03~07-17 0.7620 -0.:1)81 -0.1362. 0.0365 0.7308 0.6178 0.8646 0.5761 0.9139 0.453:1 1.0086 16 04-04*17 0.7o43" -O.i523 *0.1392. 0.0351 0.7258 0.6174 0:8531 0.5765 0.9012 0.4SQO i.00;1.s 17 os~o2-11 0.6740 -0.1714 -D;l37J 0.0316 0.72.92 0.6305 0.8434 0.5939 *o,8858 0.4521 1.0063 18 QS-16-1? 0.6906 *0.1608 -0.1382 0.0320 0.7275 0.6278 0.8431 0.590LI 0.886t1 0.4511 1.00110 19 06-06-17 0.7367 -0.1327 *0:1393 0:031/l 0.7256 0.6280 0,8384 0.5911 .0 ..8810 0.4499 1.0013, 20 07-11*17 0.7519 -0.1238 *Q.-1393 O.Q314 0,7257 0,6280 0,8385 0.5911 0.8211 0.4499 . 1.0014 Nore1 7-day JC 1 ~ =- 25% inhibition concentrati<1n. fin es1imatlo11 (lf \!1e rio\asslum chloride cancentrdtion that-would muse~ 25~: reduC!lori 111 Pimepholes gro\vch jrnlr.ulated using To:i:Cak). CT= Ce1wa1 rentlencv or the JC,, values. s = StanaartJ deviatiori of the IC,$ values-. Control limits= Mean logafll}lmic IC,; :t 2 StoiidJrd deviations converted to anli*logarithmlc Values Warning Limits= Mean logarirhmic JCi, :!: 2GV or s,..1, roriverieu to anti*lagarithniic values. SA.>*= Standard.dev1alion cotresponrllng co<he 75 " per1::en1tre cif c.v~ reportea nauon~llV by USEPA 1s, 1;"' U281~ 1 CV= Coefiir.le11t ol variation, ------~-----*~ * ' lml1'lmtlltnt.llTHliJtJ5eb:ut*n,1111 Pimephales promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture 1.25 ..c - ClJ, Cl! ;::a 1.00 s: --- ---- ---- -*-- -*--;, 0 (,!J ~ re 0.75 --- --~*- * ~---*rs--- -----..,(lo----* 0 * *---**---1)*---0---o-e*----'o::......--*-o-n-*- 0 ':p 0,._ *c: * *C u 0 bO 0.50 -- -- *;><~~ - - - E a.is USEPA Minimum Acceptance Criteria (> 025 mg per surviving larvae) e-c .c 20 USEPA Maximum Coeffident of Variation Guidance Criteria(< 20%) 0 ..... +:; s0 Ctl Ctl 'l-. l!1 *--- __ --- --*- --*Jo-- __. __ ....c0 QJ *u ... u .....e r:* 0 .10 0 . 0 . .----- 0 Cl 0 * . ii:QJ 0 ** \J 0 0 *- -- *- --- _., USEPA Upper PMSD Bound Acceptance Criteria (< 30%) 30 USEPA lower PMSD Bound 20 (>12%) 0 - ~ - --------------------*-~-*--~..--...-------- ------------ ~-
- Cl
- 10
- 0 G
- 0
-~ *- - - - -,- - - - - - - - - - - *- - 0 ._-. - * ...._ - -- - ,.._... - - - - - - * ,._,._ . . . _ , - - L-- -~ - - -
- Control Growth, Coefficient of Variation (CV) or Percent.Minimum Significant Difference (PMSD)
PMSD is the*percent minimum significant difference between the control and treatment that can be declared statisticallv signific;ant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is nqt a minimum acceptance criteria . . Central*-Tendency (mean Control Growtli, CV oi' PMSD) 5% Confidence Interval (i11ean Control Growth,. CV or PMSD +/- 2Standard Deviations) Q) CQ CD,~.,, *:*"'.* ~ ~*""'""'* ecs* .. . Pimephale~ prome1as ~ Chronic Ref~rence Toxlcant Testing, Test A~ceptability Critet:ia O~**------.- -..~r..-.~t.'-<. Source: Iii-house Cultu.re co roiica1 oe_ttirminat1cin Con~rol Gfowth ~ontrol !lro~th (V te.stPMSD Control Control GrovJth": '_Test lmg/ini1;,1 ~rva!ll (~} .. (%)
- rest* number Test dare s.urvivaJ Meat'! CV MSP PMSD CT *95% Confidericeclnforval. er* *
- 9.!!Y. c(!nfltle.iu::e Interval CT 95% Confidence Interval *
--* (%): ... . (mg/ln~i*l larvae) ;{~}. .1%1 .. cr-Z:s -CT+ 25 ci'-25 cr.,..2S CT-25 CI+2.S i 05-17-16 100 o.~07 1'._8 !J.0438 8.6 0:5966 0.418. 0,775 .. 5,9 -1.0 12.9 11.3 s.o 17;5 l .06*07,*16 100 o.*42~ ~.o
- Q.OS9i i4.1* o.sni oim *.0.781 .s.s -0:7 12.4 n.6 ,5,11 17;8 il 06*14*16 100* ~;~5 3.2 0.0{34 7~3 o:sGa 0362 *O.i73 5.6 ,(J,~ ;12.Q 11.1 4;8. 17.6
.a 07-12-16 lO~ o:s78 -ii.a* 0.0763 *l3.2. 0.569 0.373 0.765 5.!l ,o.s 1£3 U,4. 5,2 17.6 s 07*19.*1~ lQO* 0.774 10.1 *0:1os::i :13.6 o.584 0.365 0.804 6.2 *OA** 1':2.7 :i_l.S s.s 17.6 6 08-02.-16* lQQ' 9~570. ~_:., 0;047'1 ~8.3 0.583 0:372 0.794 '6,0 *O.S :i,is 11,3 5;2 -iz,11 7 08*09-i6
- 11101 0,699 3,5. 0.0827 o.s!li 0.379 12.~ s.s 1+"8 0.803 *s;S- .();~. ~i.4 17:~
B 09~ll-~~ ~11*cr 0;60_4. S;l ~.~532° 8:8 0.592 it387 .0.797... *s;8: -OA ;ti,~ l,l;~ 5.11 27;0 ti 09*12*16. lQO o.631 4.)1 o.os2o s*.2 o.s9s .0.395 o.i94 ~,1 -0.3 1W ~..l,0 S) 16.B 10 .* 10-04-16. i!)Q *o:see 3_,~ o.osos 8;6 0;594' o.401 0:788 5;6 .O;i1 ;u.s l,Q,9. 5.1. 16.6 11 11..01-16 lqo* 0.67l 7,7 0.!)~27 9.3 o.s9s 0.!!0i 0.790 ?.1 -0.7 1*J,5 10.8 s:i. *- ~6.4
- i2 12.-1!6-16* iolr Oif1~7 s:s O.l167 18.9 0.599 .QA13* o.7s6 s:1 0.0 l~.4 11..2 4.6: 17:8 13 01*1Q-i7 l~O .o;s~1 .7,;i 0.0629 10.7 o.~i)o 0.414' ii.is~ S.1 , *0*'1 1~,3 11;2 4,6 i7.?
ll'l 02*07*17 10(). D;!~79 n.s 0.07~1 13.0 0.5~1 0.420 o.763 l?::i *0.3* 12;0 lV'I *'l-8' 17,9 15 03-07*17 100 0.638 6.2', 0.0662 .10;4 0.S!lO 0,4il< 0.759 *0,11 .\2,0 (;.:Z : 11.2 4.7 17.} i6 04*0~*17 19<j Q;620 5.S 0:991~* VUl" O.SQl (J..122 0.761 ~.3 *o,6 1:\,9 11.3, 11), i7.9 i? 05-~*17 100 o;7i!! * !>;!;, *o.o7Q2 io.11 o.596 O./ll6 tJ:p& 9.2 0,6 ~~:~ ~l.4 S;iJ 11:1}~ 18 05-16*17 lQQ J>,s93 ~:s: O.OSll 8;8 .o.~ b;4311. o.771 9,0 -o:i ns ll.4 s.*o i7.!) 19 06*Q6:17. 100 0.608 ' .i,.z* M>5112 .8;9 *0;601 0.43/l Q,768: s.a* :_ip :l.Q~ 1_1.p 5.2 16J~. 20: . _07-11-17 .100 1 0,iu: __ 3.3' 0.0589 :8;a o.eo9 o.li3_8. 0.781 . - . 5.6.. .0:5 . 10.7 10.S. 4" *16.B-Note:° C*;mtrol S~*,.j = l!SEPA minimum ten ~'!'eptabillty criteria~ 8!Y.G suri11va1., Conrrnl Mean Gr<ilv!h '." IJS8>A minimum le>l *c<<Jpt>blhl~ ci1tena ~ C,25 mc/!UMillni: Jirvne OI = .Ccolfltiont o{vnrlliucn tor tonu<iJ ~roWI~, IJS'"::PA m***mum cv g*Jidanc-. tfll*tia j9o* percent11i!t.: 2m*' MSg =Mimmum 11cn11lcaii1 illlie10.nte1 PMSO = Pe1~t1t inm1mum !l&nilieant"dliferenco, PMSD is a measut~ of.mt precision. The PMSD I~ 11'4 mlrlilTIUm per.c~litdllferenai bi!1wun theci>ritrcl an<l treatmenl 1foirca11 i>a docla~d tr~ifill~~Qy 11gnll1C;iu1In3 Whole effluent toxic!V !",!I. L1>wer PMSO bound de!cnnlned by ~SEl'A r16" 0;Pl!ITI!n!llZJ;_; 12%. . . *Tni> 101vei:"M!';O bound repfe<enu a Practl(al llmli lo the ;ens*tiY>lycl tha luhMl~cd *nd I> ll<?t a minimum *~ep_!.$ncc rr>wi~ 1 UD~;,,1 f'MsO boom! ntt~Pl*nc~ trMrta determined bV us£Pll 190" iiercenlllo) !c 31/V.. . * ~-* CT= .CeJltPi terid*ncv<ilM gro>,\1h, CV*or-l'MSDvalu.N; S: Sl*n~>r~ dP.y;~llc'I oi. lhe BtoWHr, Ol.or,l'MSP values. -'-'** . . .~\ ~ ff3 c:..:.;.-1 ~ ;~.:.'.! rf Q Page I offi LI*- _- l -. ! ---. - *~
- L_ ' . I PoJass.ium Chloride Chronic.Reference Toxicant Test (EPA.,822,;R-02-013 .Method 1000.0)
Species: Pimeplwles proinelas .. . PpKClCR Test Number. 28 Di"1tion vreparalion brformatio1i: *Comments: KCI Stock JNSS number: INSS \$q,°'\ Stock preparation: 50 g KCl/L". DissoJ vc 50. g KCJ in l-L MiJJi:Q water. Dilution prep (mg/L) 300 450 600 750 900 1050 Stock volume (mL). 9 13.5 IS 22.5 27 315 Diluent volume (rnL) 1491 1486.5 1482 .1477.1 147.3 146~i5 Tolnl volume (nit) 1:500 1500 l:iOO 1500 1*500 1500 Test or~a11lsm illfonitatioir: Test i11for111ation: Organism.source: In-hbilSC* cul tu re Randomizing template: U&riT CAA~ Age: < 24-hours old Incubator number and \b Spawn dace: o, *o<; - ,., .n-11:1-\"" \~C)Q .shelf location: ANemia CHM 1iumber: CHM914 Hatch dates and times: Drying i11formation for weiglrl it) 0"1-\\-n Ole ~s -determb1alio1i,* Transfer vessel infonnation: pH= 1,qq s.u. Datei Time In oven~ OMi*~ -~~ Temperature= 'l_{,i..1 oc .Initial oven temperature: ~-c. Averngc rransfer volume: <0.25,mL Date I Time our of oven: av\'\*<'\ O"\~S Final oven tefilperatu~e: 100'( Total drying time: *1.>\-t~0&...S Dailyfeetlir~g.a11d renewal informntio11: Day Date 'i\*Iorni:ng feeding Afternoon feeding Test initintion, r~new:il, MHSW or lC?rmin:ition batch used Time Analyst Time . Analyst Tim<l . Analyst. O' 07-11-17 61.c\l) H \1-\D \,{ 0 gu::i 1'...< cn-o.-..-n I 07-12-17 O'ooo . ~. l"'l.bCl ~ O&C(:) M v O'\*M:::.~ n ...~ '2 07-,13-17 Ck>.bO ntti ' !ti U" .nx. eo IX O\*l{)-t'1 3 07-14-17 ' O\oCD J'\ ,\J \ '1..Qc, \( ~ !'If d:O xi u Q4db-ri 4 5 07-15-17 07-.16-17 """-:,~ . \l v- \(""!IC \'"L~o ~ n~:=a...o 1-f ft ($".\*\"1.-\\ CS'"\*\~,..\} 6 07-17-17 Die nL6n =-tl -x\ \( L1:0{) .ti ,h OR '":>D o.&oo IA Di.*\:,-n u1- . 0' 7 0.7-18-17 .. 61 \0 '\\ l Co1ilroi i11fon11atio11: Acccpronce criteria .Summanr of test endfJOinls: '%'Mortality: (', 1. ~20% 7-day LCso 8:"Zl .g-Average weight per initial larvae: 0.1\~ - NOEC kJaO c.11~ l .Average \veig!1t per suniiving.larvae:. ~ D.15 mgllarvac LOEC -i<;c) ChV 1.o-10.8 *IC~s "'15. \ ..~ SOP AT21 -Exhibit AT2 l~I, reYislon 11-0I~14 Page 2 of6 l 1\.------ - - -- I ~-3 ftt1'it:ln1";1"tnu.1Tu*ln9S.Cl~t10"1,f11c-
- 1 Species: .Pimepha/es pmmelas PpKCICR Test Number: 2S Sur,,ival a1Ul Growt/1 Dara Day Control 300 mg KCl/L 450 mg KtI/L A B .. c .D E F G H I J I< L 0
ro !O [(I 10 10 w IO IO 10 10 10 IO 1 10 JO (O ID 10 IO ' I) (0 IQ to 10 10 .2 lO l () ro 3 II.) IQ I\) to IQ (O 10 10 '° ~u l10 CO IG rt) IO to it) l(J co 10 lo 4 ID It) IQ I CJ l C) Lt) l C) /() I t::i. I CJ !(') to .5 I() 'l{) I\) lb /0 l (j co fQ* l tJ /t) lO ID (i ID l ti lD tD l.b io to l0 ro tD Co l'O 7 I r::;., / IJ It> to ro (fl I f::J l t) ID l'D l Q. (() A= ran weigh! (mg) Tr:iy color 5,0dc:~ fc:c.sf. 1\11alys1: 'R!rJ ~ ,~~o ,s:bi *~1-C{, ~ . ~~o ,~~t \~~'& \(.;;~°' \'7£\ ,~"f:>\ \\sr:;f;, ,~:t~ \o.cP1 IJ~tc: {j£. -I -)J ll =' 1'1111 +*Larvae. weight (mg) Analyst; ~ Date: QJ----l::1 - '?9-'>Ur. ~1>*~"' '}~~\ . . ~4.~ d-"5'i' '~\~~ ';}9-o,,c;' '}~'7\\ 1J.4:1$1 ?P\ r°'°' gfl..~i C= Larv:ic weight (rng) = ll-A lfancl calculntcd. t...S'-\ "1.:fi 1. 1.."!> i.~$ '"1. O';::) j.ib ., *1.'- 1.loQ '1,.~ i...'\b ,.*t~ "\.~\ 1\n:1IJst: ~ Weight per initiarnumbcr ofl:irvoc (mg) = C / lnltial number ofl:irw1c .*v..: ~r:;, °"~ A.~"t) I\~ ,..,,J:? ,....o'r A.(? I\'? I\~ I\,;S'° flan<! ('alculnlcd. v "~ !'\: ~ Annlyst: J\. o* ~* o* o* - -...- *~ :'q o* /\. o* o* o* o* o* I.'.)* ~* Avcrnge weight per initial Percent l *. ' uumbcr of lnrva.c (mgr rcduclion rrom control O.lt~ ) ~ , - i 0.1..:\ \ -;h°'. .1. e.11.1 - \,I\ 7. (%) _*';.~_'I *1- * -* -~ .- Comment codes: c =clear, d = dead, fg = fungus, k = killed, m = missing, sk =sick, sm = Llnusually small, lg= unusually large, d&r =decanted and returned, w =wounded, Comments: age 86of93 SOP AT2 I ~Exhibit AT2 I.. I, revisio11 11-01-14 Page3 of6 Species: Pimeolwles promelas PpKCICR Test Numper: 28 SurviMl and Groivtlt Data Ifay 600.mg KClfL 750mg KCl/L 900 rng KC/IL M .N 0 p Q R s T u v w x
- o. JO JO 1'0 10 10 10 JO JO 10 IO 10 JO l
JO q*-\ '1 *c;l q'-< /D {'0 JO to JD 10 . ID IO 2 . £,A. IQ ID IO It> Co I() /{) /.Q '\ iO q 3 q *~ 10 to i() ~ l i) l~ IO ID IC) 4 '..o\...' ~G\. . ~ 4 ti:l JD It:> lCl ID ID* 10 lD (1) s 8 .£ 5 ID 10* lO ro ,g ~* £IA. & ~"lA ~ '2:~ ~ s s'*"'- 6 /{) It> . ,J... '\ ID g .., ,.( g g 91'A 3> '\A 'i \~ 3 ~ \l..<t 7 lt::J . l"b G\ ftl .... ~". \ g & :;;"- ~ .2l' '\ 3 \l..fp. A.., Pan wcighl (mg) ,* Trny color code:: *~ore.~l ~2.n ' Annlyst: * ~ Date: ma-~~-IJ * * \~~d- \~ *'*\l \lo;9.l.D \f;)$~ \'3~ ,09'.e \4.\\ ,~~'b \~J:l>i \5:19- \c;,~o \l.\."(1 B = Pnn +Lanae weight (mg) Analyst: 1i1'tJ Datt: Cf.1-~o-1:z* ~~~ *~ ~~\!) . rf_d:;;3 '}.'().'fl\) ,~~o.. .t;;.\ ~~ \~~?- 'J.\*Ul-1 n~°' '\:'\~l.\ . . \\?t\ \\,n;lt) .. c= Larvnc wei~ht*(mg) = B-A llnnd clllculntcd. -1.'b c ~-'°°' t...:~ ,.n $.~ 1$.~& i.\.~\ 't..~'\ l.. ~~ '2,..U,. "t.sg \ .\.::, Annlyst: ~ ',Wcight'(lcr ini!lal. number of hll'\':1.e (mg) =C llriitinl number oflnn*ac 0-. 6' ,.,,,rv ~ ~ t.;~ ~ ()'. v, Hand c:ilculntcd .. # \.,SI -...9 "9 ~ " c..,g. !)..,; 0" 0 rV' l\r ..... Analyst: .~ o* o* *o** o' o* Q'. o* o* Q' 0 o* Avcrngcwcight per. initi:H , Pcrccnl nnnibcr ofhirvac (mg) reduct.Ion o.lc.&\ ~ .. $7. o.s'--\ '\ l.i..'\ 7. (). 1.\"1 1.,;c'.\.io 1.
- rrurn con lrol
(%) Comment codes:* c"" clear, d .=,dead, fg =fungus, k = killed, m =missing,. sk =sick, sm =unusually small; lg = unusually .large, d~r = decanted and .retumed, \v = wounded.
- Comme11fs:
age 87of93 SOP AT21-ExhibitAT2l.Uevision 11-0J-i*,l l Page4 of6 Species: Pimepkales promelas l'pKCICR Test Number: 28 Suri~ival - . mu/ G1*0111th Data Day 1050 mg KCl/L y .. z AA BB 0 10 1.0 IO LO l &T.~ 1.,'-l~ ,~ . lo. 'le\ "2.<ll . 2 *I.- s'"" l c_ 3 *il.' w.. '2J'. I 5 *'-l s '-\ 4 ~. ,_~ """'-
- > 3
\ ~ ~;>\ ~ 5 '"l.-" D \ 6 0 \ ' D LJ,. ol/I, .J .o 0 l a A= Pan weight (mg) . Tray color~ faresl *('F ec Analyst: * * ,~~(:\* \'ti .c.P'\ \i..JJf.,":i *\1.\:55 Date: Q{o*l~-"lf
- 13 =Pan+ Larvae weight (mg)
Analyst: Date:
- ')Yn\
01-ac')-~:J __.,., \~* ~,, C= Lnrw1c weight (rug)= 13-A llrual calculate~, --i... o.-n Analyst: ~ Weigh< per initilll numlicr oflnrvac (mg) "' C / Initial 11111nllcr of lnrvac 0 D ""'C\ Jfa111.J crilcuJ;ifcl.l. Analyst: ~ o* """ Cl \ Avcrngc weight per inilinl 'Pcrccn! number onan*:ic (1!1g) reduction from control o ,c'-°' ql:!l 7. (%) Comment codes: c =clear, d =dead, fg = fi.1ngus, k ;=killed, 111 = missing, sk =sick, sm =unusually small,. lg = unusually large, d&r = decanted and retumed, w = wounded. Commeuts: SOP AT21 -Exhibit AT21 .I, revision 11-01-14 Plmepltal~protnelas *<;;hr'ariTc Ref~renc;~ Toxli:ant terl EPA~S21*R,o2~(li3 1 Method ~QOO:~ Quality Control V'erlflcatlon*i;>f Data E11ti'y, Ciilc\jlatlons, and.Statlstkal.Analyses. TVttnymbet:._ _ _ _ _ _.,..,._ _.,.....,,.....,..-1_1 To1tdo\oi'..:..*_ _ _ _ _ _ _.;..Ju"-ly~*ll_*_*l_-8.;../_2*O_l_l. l ~~f"n*Odktn t~(ll ~C! A*pJltlflfl * !!'utllrilt"lbfr.nf: l~l'V!_** f'l"llbUl1'lb!JC'. lftWio_f. A~r~"*~1f111.fmc> ll71'f.-.;Ltt¥H w*f!tt*fm*r ... la~IJlf"'4l&f1tlm1)ll W1!1t.t I Survtvtnr-* Mtll't\fi!*l&ht/~llMtffit Co*tfTde-1'11 or\'!fr.,~ ... W*'*"'(lf\ltt,l1tl411'b*1Ct rill**l'l1u1.,;nJI~ Muntttl1h'1 tn11W ~bo.J1t.rviill*('"ll numhraf,~M(rnd* OJ,*qw1..i~ ...........-, *,..;..,_**M"'**;>D*~ ,,,m_{'."d ""~~~11*r,.a l"'ll ~mw.,,11tfttw:1iatb11 !l;I r*!"flt "d'!HIM ft~ml C~rl~l~l l~f *p; .. g. 10 ,,JO .IS.SO 2z.a4, - *G:Sa* *Q.68q* '3.6S.t *10 *10 15.SS ZJ.27' ,739, o.n9 0,739* .u Not ~ot>llc>llle Caln~rD~ Q:7H i.3 :100.0 0:715 c ,1Q !O 16.28 2151 7:2:!. '0.121* 0.713 *o .10' '.10:... ' .. 15.80* -i2,85 7.0S* .0.705 0.705 ' - -<;. *10 .. 10 14.SS n.s1 7.lll o.1aJ. 0:703' ~F' *10 .. ur
- ll.Gl :U:36 ,7,]?, *0.77'! 0:1"3 uo.g 3ou. . :.G .. *o;m; *li.7~i :.3
,0.726 0.1'1} u -3,!J 10 .10 15.&9 2295* 7.2* *If, 10 JO* .. 1$.81 2Ml .7.60* Q,760 .. ,- .. D.7GO. *4SO I K* *10 10* 10* '10 *10 1a '16.31' 16.05 15,76 ZJ.st* *;:?!.01 22.99 .7,S6; .6.96" 7.23 .- 0,75<' 0.096 "0.723*' Ii.in J~ 0.7S6 D.6!16' 0,72.l' ioo.o 0.n7 3,<i *ij .. L. 10 10 '_... lS.67. .?2.98 .. ' 1.n 0'731 0.731. 'M. *:10 .. 10* , lSJl2.* .n.12* 7.30 .Q:7l0. 0,730. . ;N. 10 10* 14,47*' H.16* 6.69 0.669' 0.669,, ~ o,6~~ ;1.4 !J7.5 ,Q,t;U J.l 4.i. ,Q'. .10* 9<. 16;25 22:.ll *6,07' 0674* . 0.607 p 10* .10 1533 .. ll.SO* .7.17' *0.717 *0.717' a; 1,Q* 1 *n.YJ lt3'> *s.~1 O:rl1 \),~'il';I, !! 10 *1> 1.S.90 21;88 *5,93: i:t.115.4 0598 75U ..S. 10* 8* l4;ll .36.92 4.l!l 'il,60! 0.7llf 14.J OA61 is.a *0.549 ,-1i.i 2i.<J T. .!ti' 8 JS.SB 2Ul: li.09' 0.761" 0.609' lU . 10. 3 . ' '1534 17;49*.* 2.JS' o.n1 a.us IJ, JD. .3. !5:12 17!94 Ul* 0,740' *0.222 ~oa 0.'11:?? iri,l 3iJ;~ ~o.~11 16:1 l~f.~ w* lO 3, 15.H* 17.91 2.58* 0.&60 D.:?SS> .*.ic. .. 10 .;, '14.91 16.70 0,577' 0.173 Y'.. 10. *O. ,0.00' .o.oo l.;13' *o.oo, ", a.OOO* .*--~ - -~- ""- - 0,000* lOSO* '.z. lO* ISM 16:~1 0;77' o,no* 0.770 D.~ 0.077. u *0.1)19 ~00.0 ~1.3 .M' -10 a CJ,00. 0,00 MO' o.oua 'o.ooo 881 10 1 ' 'O' . '0.00 o.oo *a.co* *.o.ooo
- o.ooa Ounol!l11i M50 '1alun: . o.osii9. MSO= Mfiilmum s.r&niflcanttitrrerence PMSri, 83. P~*"°*" fi*r~n* Mln!miom Sif,oiPi*ill Olfl!'f*nco
*PMSD 1.i 0n\~3Stlte ~f 1en P'ect51Cm. Tti~ PMSO lS thO minimum perc~ntdiffer<!ntl! bet\V..e~n t~11C1?rltr'?I ')n'1 h';'~.~111~n~ th.it =ari ~-~ ~~l~~~d .stall~\li:tlHV 11c:11ill~nt In a Whole ~Hluf:nt tox;~ltV tad * .lower PMSO boun4 oiir~rmli1.iJ1 Lv USEPI_\ 11001 P."*<<ntll*I ;o *mi. * * * .\Jpp*1 PMSO'bound d~term1neiJ bv VSEM !90.lh po1<en111oi =30%. . tcwor ood uppt!t PMSO ~oUnd>were~clcflnlnod fr,om th ..,lOth *nd 90th per<ontlle, rospoctlv*IY, ol PMS!> 4*to li~J!t EPi* Wti-(n!**loi>or.llOrl VarlabtlltVSttiW (VSEPA, 100 lo; IJ~liM, ZOO lb). *Tha fow.,rPMSD boui\d tepte"enls i ~fyu;tf~;il ilmfl ta the ~cniltlvtwof the tl!tt mc-lhcd ar1;d h. f10t ~ ml11rqw1i\ .!¢11Pli1f1Cl;i ~rfl~rtii; * * ~ ,. . Statistical AmilY#es
- S1arroa1.e; 111.112017 Tesl ID: PpKCIC<R S:implEi.IO: 'REF*Ref.ToXtcanl End Dal.ei *71ia1io1t Lab:r[): ETS*Enliir. Testtng*So1:. Sample Typo:, .JCCL-*PotoGsium ct1ioricte Pr.olo.ccir: FWC.HR*EPJl.r821*R*02*013 Te61*Specles. PP*Pimoplialas promalas Conc-m /L
- 1 2 ,3, .,
.D-Con1rol* .1.0000* 11(JOOO .1.0000 1.0000
- 300 .f,0000 110000. 1.0000. 1.ricioo.
.4SO .toooo* 1c0000 , 110000. -1:0000. EOO *1;0000* i.oooo 0.9000 i.oot.m .. 750 '0'.7000* 0.:7000. D;8000 0.8000* 'YOfl 0;3000 *o.:moo* 0.30{)0 0.3000* 10f?O 0:9000 .9.1000 0.0000 0.0000
- Transform:.Aresin.Squurc"Root** Rank 1'-T.alfad Number Total
.Conc.mg11:. Me:in N-Mean Mean Min. *Max , .'C\/"/o N .sum Critlc::il :Resp . Number. *D*C,qi\lfol 1[000(,'I 1*0000'
- 1A1'20 1"<J120* *.M120 . o~.oo.o 4* *o* 40'
;)tJO l\ilOOO: 1.0QQQ 1*,4120 141~0' 1.4120' o:o:rio. 4* ia:oo 10.00 D 40 450 1-.0000 1.0000 :f,<1120 l.4120 '1;4.120' (),l)QO. ol* 1B,O{l l0,00 o. ~o *GOO 0~750 0*9750 f.3713 l.2490, '1:4120' '5,94::! *4, 16;00. 10,00 1 40 '750 .Q,/500 0.7500 J.0492' o,9912. 1.1071. 6;383 *4~ '10.00 *Hi.DO 10 dQ *9oo :U.3000 :iiaooo 0:5796: 0 57g5* o,57!is, .Q,000 4: *10.00 *10.00 26 40. '1050 0:0250 0.'0250 0.1995. 0:15BB 0:32.18 '10.6~0 *t 10.00 10,00 $!} *~O Aao:Ulary Tests .Critical *Skow Kurt Shapiro'Wilk's Test inaicares non-nonnardlstribullan*Ci:*<= 0.01) 0,86999 0,69&* .OE-14 2.67033 Egualllj/ of variance cannot be confirmecl
- HypotheslsTest.(1*t:lll;'.0.05) *NOEC : l:.OEC. CftV:. >TU Steel's Many-One Rank Test 600* 7.50 *670182 TreatmenlS*\/s D-Controt Maximum l.:ikallhood*Probit' Parameter. ... Value . SE. 95% Fiducial Limits, control Chl,Sq Crltii:al P.:valuo Iler Slope. is.aos; .2.os2es. 11.e435. 19;9299* o 0;9401;? 9,46773 *0;91874 ;i!,91.528 0,06'295 .3 tritercept . -41:214 B.02.62; * ,s:t.t3 -20,.qs!i TSC\l ' '1.1).,,.,---------::;;:~---.
Point Probits .mg/L 95%'Ffduclaf.Llmlts*
- 0.9 ECO!' ' 2,574 . 587/28 516.855 535.575 EC06 3.355 648.2A9 588.51 & 6S9A68' ,6.s EC1il 3.716 6eu9a 6ao. t59 72Q,(l4~ .0:1 EC15 S.9G4 108.01 65!.':ss2 74z.,e6e '
F..C20 *USOl:,.,..-....,...,..,.. EC95 6.645 1044.28 !180,513 ti40.24 1 JOO 1000 10000 EC99 7_'32& 1.t 52.69- '070. 7 l 2S'7'.68 Dosa mglL . o.e r:t.a .,,o:7 ~ ' . ~*O,G
- l
'(fl 0.5 S 0.~
- Cl.:l o*~
,0,1 0 -~~---~£-.---.~o----.----,-----o..----+- 8 ~ m ~ o ~ 0. -~-*'\' ,-....,,,,,- ..~ Statistlc~l"Analyses, - ~Larval Fish Growth:ai1d Survival Test-7 Day-Growth: Start Datec 711~12017* '"(es't'IO: PpKCICR Sample: 10: REF-Renoxic-.ant E11d.D~\e i'/lS/2017' lab' ID: ETS,Env1r. lesllng-Sot Sample Type! KCL,Polassium chloride *Ptotocol: FWCHR,EPM>2H'~'o2;fn3* Test.Species: PP*Pimephales promelas. Conc*m!:llL 1 2* 3. 4 o;cont!'OI* o;s84o *0.7390 '0.7230' 0.7050 300 0.7Q30 IJ.?730 0.7.260 0]60\l 450 0,7560 0.6960 o.n:io o.73to 600.' 0;7.300 0.6690 o.eoro 0.7170 750 il:s;os.6 a\ssl.io 0 ..:810: i:i:6aeo
- 9ll0 0.2150* 0,2.220 0,2580 0,1730'
>ioSO 0,0000 o".atia o.oboo O,OQOO' *Transform: Untransformed- 1-Talled tsotonic, *Conc*m£!1L Mean N-Mean Mean. Min *Max. CV% , N. *1*Stat *Critical MSD Mean* N-Moan o,con1r01 0.7128 1'.0000 '0.712~ 0.6840' '0;7390;' 3.;321 *4 0,7266 1,0000 300 0.7405 '1\0389 0.7405. :0;7030 0.1730 4'308 *ij ,1;~7'9 :two IJ.0589 0.7266 1:0000 450 *0:7265 ,,0*193* 0,7265 :016960 _0;7560 3:.402 -4 *0:534 2:2riri 0.05(l9 0.7255 :o.999S 600 0,6806 o:es51 0,6808 0,6079 *0.1300 8,166 '4 1,;;?4:.l ~.:t!99 o.osas *0,6BOB- 0:9369 7p0 *0.5493 0,7706 '0.5493- 0;4810 0.6090 1'1,622 4 0.5493 0;7559 ODO 0;2.170 0:30il5 *0.2170 0.-113_0 o.25ao. 16.065 **l o.21fo *0.29B6 1050 0:0193 Oi0271? o;qtes 010000: o.ono 200.0.00 4 :0.0193 *O.OZ65 .Auxiliary.Tests. Statistic - *Critical Skew *Kun* SllllpirOC.1/\lilJc's Tes~ Indicates noi:mal dlstriblilion*~1=1->*0:01} *0.96535 *Oc844 -~o.5692 0.13191 Beltlell's Test* lndleales equal vanance.s (p--= 0:43) :i.111692 1.1.-3449 HypotheslsTest(1*tail;0.06). *NOEC LOEC * . ChV ru* **MSDu MSOp MSS. Mse* *.F..Prob *df 01mnett'$ T.ist * .soo ">600 . *0.0589'1 0.08265. D.00261 :0.00132 *0:1716 3; 12 . Trealmerils \is D*Contiol* - *Lrnear.lnterpolallon (200'Resamples)' Point SD* 95%.CL(Expl *Sliew ICOS ii6a:n 45:63 431.7S 669,75-* CQ,"1430 IC10 *s:fo.56 .30:26 507.55 i,;97,~5 *0.7644'* 1c-1.s 67!2,00 25.0B 579:B6 7Sf;~o *D.1.576. L\l~-----'---;-=~---, 24,64* sss.3:> .7B615 ._0;01so 0.9
- 1**
1c20 713,44' IC25 1a*1,93 18;96 674;59 7'05.48 '-0.7990 IC4o B01.14 9.18 765-41 822:ss -'o,sfos o.e
- ICSO *BSS.911 6:84
*8Ci7.65 *849A7; -D.4666'- ,fl>. *0.7, 0_8 T *Ill '.g_ ~-5 ~ ..._g. 6,3 I?! 0-~' 0,2. 1,).1 !J. a oh..,._,__..._ l)o.-.:!" a 500 io*ao' i5,00 oose-mglL QOSC"ReSpJ)nSe Plot 0:9~---------------------. 1-'!all, 0;05*tiNel ~f.slgnrilran~ 0 *0 C* *O age 91of93 Page 5 of 6 _, *----/- ~\ tn111r11nm'!nl.d'rt11~ Soltnfan-.lra:. ' Species: Pimeplwles promelas PpKCICRTesl Numbc:lr; 28 Dailv Chemistry: Day (Annlyst id1:ntlficd*forcuch duy;.ocrformdd pH; D.O. nnd comluctivity measuremcms only;) 0 I 2 Analysl H.S r*:r . " ( .*._- .. J f - ,",:::- ----- . ~ Concentration _Parameter :.,... __ .;;.~- .... ~-~-- < , .;:..\_.,, - . . . .- . ; . * .: pH (S.U.) 00 (mg/L) Conductivity . -,.I .--;"°">--:"*--~*- ---*"' (umhos/cm} ~-. -* :\u .:._;~:; ~i CONTROL "Alk:tlinily - * "P ~ ) Ong C:tCOi/L) L -.,. ,:_'.*~j *H::mlncs*s (111g CnC01/Ll *Temper:ttu_rc (0 q 'l.'-\. s "1..-\-1..c> pff (S.U.J 7.grp 1.51-DO (mg/L) 4.6 ... 7.-; . .* 1-.1 *, 300 nig l,:CllL Cond11c1ivily \ I : (umlios/ci11) I . "< ... "-:'l.l{.!) '1. '\.~ '"L PU (S,U.) 0.0S" -:\.se 450 mg KCl/L DO (mg/L.J 7-R "'1.z. -~ ~ ... : Concluc111'it1* C1-tmhos/cm)' ----- ' _J' *Tcmper:ttui;c (°C) pH (S.U,). DO (mg.IL) 600 mg KCl/L Conducrivily ,** f, * ..=. iumhos/i:m) -' }o/O(l I c "- .* * *Tempcrature_(~C) I.'{. 5 °1.'l. ~ ."\..**\,.'$ 6.0(0 8.oo '1Ae '~.Sl '8.n ,.. 'f.3 'DO (mg/L) 750 mg KCl/L Conllnclil'ily i"' .. , - *,. -~ - ..-- -1. 1~10 ,' ' 7~-5~ (11111hos/cm) . ~ _.,*l ,, ---~ ;.- i *~w 1-*-----~' "'fcmpernturc (0 C) l..l\.\o 1..1{.4 ""L'"\* ~ pl-J (S.U.} '°}.('.\":}. "::l.S\o DO (mg/L) 'l>O '1.3 900 mg KCl/L Conductivity r*-. *-- ~ - " :*~~~-.- -*-- ~ -~-:J.! (1u11hos/c:111J ~'....... ,.._. ,:_ _ :_ - - .1 'AoO ,. ' - -- ... -- "Tcmpernlllrc {"C) ".l..'\. ~ '1>-\..s pH (S.U.) 0.oi:s .1}~8 ":.)-.5").. DO (mg/L) {3.0 (p-p '+~ W50 mg l{Cl/L Conduclivity ;r - .. ~_.-,,.-:,'*~~-; 2200 e '"' (lllnhos/cm) !i&Q) -*- ,, '2* "Tcmper:iture (0 C) i."{. ..,,~ :1 Initial ll'inar Initial Final' Initial Final *Tcmpcralurcs perfom1cd il11hc titnc of lcsl initiation, renew~! or 11:r111i1101ion by the onalysl idcnlificd In the Dnily Renewal lnfon11atiol1 tablc lbc~tcd on Page l. /\ll:alinity and hnrdncss performed hy the annlyst identified on the bench shecl-spccilic forcuch analysis ;ind-lnlllscribcd* to I his bench sheer liy: --.1.61+----'* h1U*111nd1rnt *
- age 92of93 SOP AT2l - Exhibit AT2 I.1, revision 11-0 l'-14
~ *1!:.1 ::::JJ Lt*::a (F Page 6 of6 1 1 '=J I l _,;- .. *~-/ Envlranmw1a.f1"sllr.9S-.Cluuans,lnc... Species: Pi11zeplurles promelas. Ppl<CICR Test Number. 28 Day (Analyst idc111ifrcd forcncll day. pcrform~d pH. D.O. and conduetivily mcnsurcruents only.) 3 4 5 6 Analyst *'tv .O:i=.v- ~t - .~~'- ~L..- ,\ CONTROL '+.v5 0.o4 oo <mg!LJ A.o +.hr "3.c -=?*'-" f..5 JOU mg l(Cllf.. i ;,, '; *1.* , _;;:- -*.~I r ...--....--- I ..; - - ~ ----*. *-*-** - : .. ..:~ ... ~ ... *- _..,.,., .. _ 450 mg KCl/L l\ld I "'"' r "b-\. \.\ pH (S. U.) ~!) :}- -:1. lt1*'+ DO (mg/L) t::l:n °1-P\1 *~~.-:.--* --:_~-r _., f;_-/ -::-: - .....~ 600 mg (\:CllL Co11dudivity l"1L1. O I :,,- ,,,.c (imilios/cm) \ v.,. ;f.t ~~ *<<Tempc~ntnre (OC) ~'-\*& ...i "'L4. s pH (S.U.) ~P(.} '1.'1-D .. 'd.ol DO (mg/L) 0.n 'Y:.O 750 mg KCl/L Conductivity l...-*-:i.- ~ - --r. L ~ . ___.:_~ __.
- .-;-~ ;, t --~:- ""?-:::~~: **r-.,~,
(11mhos/cm) '-=:l -iu "'"tem pcm tu re ,1 f! (oC) I,.~. 11: 1.4.1 . pH.(S:U.) '1-.l\0 "DO (1i1g/L) .8.0 'if..o 0.o JO"'*. -"';?':-:- -:i l 900 mg l<CllL Conductivity l ~-:'.J:.*". . . .I 'loo .!, ,{L:) -1°110 t --:------..... :: ** J ---- ~ J (umhos/cm} t:::.J v ~: **- -.-.;. !i_! .-.-- ..,_ ____- ___ ,! 1 '.Tempcrnture -; , , c. (vC) L."\ * ~ '13.0lo DO (~ng!L) (J ,0 r~ ~:"'.?;-;~* :i r .- - .. '~:---~ . ~'\ lO 7* *. , IOSOn1g KCi/L Comluctivily ! ::".
- .* ,*.1-:-
(um hos/cm) (!\ \.. '~?.' ' ----~.,) l*-~ ;} * """ - ..:- - . '"Tcmpcr:\ture 1.l\. ~ 1J\.S ("C) Initial Final Initial F:lnal Initial Finiil Initial Final
- , *~ mtlires perfonncu nHhcliflle ol'tcsl ir1itiation, renewal or.1cm]in;1!ion by the analyst*i~enlilicd in the Pail}" Renewal lnfor111a1ion table located on Page i.
,,u.~11;.il.ini and hnrdncss.perf.nmcd by 1hc on~lys1 identified on the.bench*::hcct spccilic for.each analysis and 1runscribcd 10 lhis bench sheet by: ~-' ~. age 93 of93 SOP AT21-ExhibitAT21.l, revision 11-01-14}}