Text

NPDES Permit No. TN0026450 - Discharge Monitoring Report (DMR) for August 2017
	ML17261A197
Person / Time
Site:	Susquehanna
Issue date:	09/13/2017
From:	Anthony Williams; Tennessee Valley Authority
To:	; Document Control Desk, Office of Nuclear Reactor Regulation, State of TN, Chattanooga Environmental Field Office, Division of Water Pollution Control
References
	TN0026450
	Download: ML17261A197 (101)
	v • d • e

{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 September 13, 2017 Chattanooga Environmental Field Office Division of Water Pollution Control 1301 Riverfront Parkway, #206 Chattanooga, Tennessee 37402-2013 TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR August 2017 Enclosed is the August 2017 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period. The final report for toxicity testing conducted July 23 - 28, 2017 is enclosed along with the corrected July DMR for 101 T. If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843~6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of iny knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and im ri onment for knowing violations. on illiams Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555

PERMIITEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ _!VA-SEQUO~~UCLEARPLANT ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Addres,L _f.Q....BOX 20QQ_ ___________ _ ___ _(lNTEROFFICE OPS-5N-SQ.Nl _______ _ TN0026450 _ _ _ _§ODDY - DAISYJ.N_]73B1.._ _______ _ Fa@_~A-SEQUO~HNUCLEAR&NL ____ _ LocatiO.!L...!:!AMIL TOJi.COUNTY_ - ATIN:Millicent Garland PARAMETER IX QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM TEMPERATURE, WATER DEG. SAMPLE CENTIGRADE MEASUREMENT 00010 1 0

PERMIT EFFLUENT GROSS REQUIREMENT*

TEMPERATURE, WATER DEG. SAMPLE CENTIGRADE MEASUREMENT 00010 z 0 PERMIT INSTREAM MONITORING REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & SAMPLE UPSTRM DEG.C MEASUREMENT 00016 1 s PERMIT fritn\\:fr EFFLUENT GROSS REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE

- - - - 11!'!1

. 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT.. Req.Mon. MGD EFFLUENT GROSS REQUIREMENT

DAILY MAX FLOW, IN CONDUIT OR THRU SAMPLE 1799 TREATMENT PLANT MEASUREMENT 03 50050 1

0 PERMIT Req. Mon. MGD EFFLUENT GROSS VALUE REQUIREMENT MO.AVG CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 50060 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE - C, RATE OF SAMPLE 0.3 62 CHANGE MEASUREMENT 82234 1 0 PERMIT 2.0 DEG EFFLUENT GROSS REQUIREMENT DAILY MX C/HR / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all aUachmenls were prepared under l direction or supervision in accordance with a system designed to assure that qualified Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inquiry of I e person or persons who manage the system, or those persons directly responsible for gathe pg the information, the information submitted is, to the best of my knowledge and belief, true,

~

/ 101 G Dy 31 DIFFUSER DISCHARGE EFFLUENT

- - NO DISCHARGE D NOTE: Read instructions before completinq this form.

QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS 42.1 0 31 / 31 RCORDR 04 Req. Mon. DEG.C. CONTI CALCTD DAiLYMAX NUOUS 30.1 0 31/31 MOD ELD 04 30.5 DEG.C. CONTI CALCTD DAILYMX NUOUS 2.3 0 31/31 CALCTD 04 3.0 DEG.C. CONTI 'CALCTD DAILYMX NUOUS 0 31 / 31 RCORDR CONTI RCORDR NUOUS 0 31/31 CALCTD 03 MGD CONTI* CALCTD NUOUS 0.017 0.043 19 0 25 / 31 GRAB 0.1 0.1 MG/L FIVE PER CALCTD MO AVG DAILY MAX WEEK 0 31/31 CALCTD CONTI CALCTD -~ NUOUS / _L' TELEPHONE DATE ite Vice President 423 843-7001 17 09 11 Site Vice President accurate,and complete. I am aware that there are significant penalties for submiUing false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following injections occurred: Spectrus BD 1500 (max calc. 0.03 mg/L, limit --0.05 mg/L) and Spectrus CT 1300 (max calc. was 0.025 mg/L, limit -- 0.05 mg/L). EPA Form 3320-1 (REV 3/99) Previous editions may be used. Paae 1*of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Na~-~~-SEQUO~~UCLEARPLANT ___ _ Addres,L _.E.Q,_BOX 2000 ___________ _ ___.J!.NTEROFFICE OP§.:§.N-SQ!il _______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 T MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 ---~ODDY-DAISYJ.ij73BL _______ _ PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Faclfily_..JYA - SEQUOYAH NUCLEAR PLANT _____ _ Locatio..!L _l:!AMIL TOli.COUNTY.._ _________ _ EFFLUENT ATIN:Millicent Garland 1 MO r~RING ff:.'~ MO, DAY From Y~;R J 08 _ 01_ To I 17 I 08 / 31

- - NO DISCHARGE D NOTE: Read instructions before completini:r this form.

PARAMETER x QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM IC25 STATRE 7DAY CHR SAMPLE Monitoring CERIODAPHNIA MEASUREMENT Not Required TRP3B 1 0 .PERMIT 42.8 EFFLUENT GROSS . REQUIREMENT MINIMUM* IC25.STATRE 7DAY CHR SAMPLE Monitoring PIMEPHALES MEASUREMENT Not Required TRP6C 1 0 PERMIT .. 42.8 EFFLUENT GROSS R.EQ(JJREMENT MIMINUM SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT v-- )* / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ' '""" """' """"" '~"" 'M' OO~m"""' "' ""'~momo - ,,_oo ~""' r. ~., Anthony L Williams direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry oft person or persons who manage the system, or those persons directly responsible for gathe ng the information, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in August 2017. EPA Form 3320-1 (REV 3199) Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL TELEPHONE DATE 423 843-7001 17 09 11 I AREA I NUMBER YEAR MO DAY CODE Pam'! 1 nf 1

. PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ _!VA-SEQUO~~UCLEA~LANT ___ _ Addres,L _E.Q,_BOX 2000 ___________ _ ___ _(lNTEROFFICE OPS-5N-SQN) _______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ TN0026450 103 G MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 ___ __.§.ODDY - DAISY,_I.N_l73BL_ _______ _ LOW VOL. WASTE TREATMENT POND Facl!l!Y_.JYA - SEQUOYAH NUCLEAR PLANT _____ _ LocaliO.!L...!:!AMIL TOJi.COUNTY_ - EFFLUENT

- - NO DISCHARGE D...

NOTE: Read instructions before completin~ this Form. D y From 31 ATTN:Millicent Garland PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7.0 7.4 0 6 / 31 GRAB MEASUREMENT 12 00400 1 0 PERMIT 6.0 9.0 SU ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 8.0 8.0 0 1 / 31 GRAB 19 MEASUREMENT 00530 1 0 PERMIT 30.0 100.0 MG/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH OIL AND GREASE SAMPLE <5.0 <5.0 0 1/ 31 GRAB 19 MEASUREMENT 00556 1 0 PERMIT 15.0 ~0.0 MG/L ONCE/* GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 0.779 0.887 0 5 / 31 IN STAN 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD

- - - O**

ONCE/

INST AN EFFLUENT GROSS REQUIREMENT MO AVG DAILYMX WEEK SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT v---*~

/ / J NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ' """' ""'" ""' * """" '" Oorum'"' '"' "' ""'m"" ~* ""'"' ~f. ~ TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inquiry of he person or persons who manage the system, or those_ persons directly responsible for gal ering 1te Vice President 423 843-7001 17 09 11 the information, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name_~~-SEQUO~~UCLEARPLANT ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F - FINAL Form Approved. OMB No. 2040-0004 Addres.L _f.Q,_BOX 2000 ___________ _ ___.J!.NTEROFFICE OPS-5N-SQ.Nl _______ _ TN0026450 ---~DDY-DAISU~738L _______ _ Facjfily _ __IYA -_fil:Q!.JOYAH NUCLEAR PLANT _____ _ Locatio.!L.J:!AMIL TOl:!...COUNTY __________ _ ATTN:Millicent Garland From PARAMETER QUANTITY OR LOADING AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE CENTIGRADE MEASUREMENT 00010 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE, WATER DEG. SAMPLE CENTIGRADE MEASUREMENT 00010 z 0 PERMIT !\\'.*****"" INSTREAM MONITORING REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & SAMPLE UPSTRM DEG.C MEASUREMENT 00016 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. MGD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 50060 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE - C, RATE OF SAMPLE 04 CHANGE MEASUREMENT 82234 1 0 PERMIT 2 DEGC EFFLUENT GROSS VALUE REQUIREMENT DAILYMX SAMPLE MEASUREMENT PERMIT REQUIREMENT MINIMUM

- - - II:***
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110 G RECYCLED COOLING WATER EFFLUENT '

- - NO DISCHARGE I xx I NOTE: Read instructions before completinr:i this form.

QUALITY OR CONCENTRATION NO, FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS 04 REPORT DEGC CONTIN CALCTD DAILY MX uous 04 30.5 DEGC CONTIN CALCTD DAILYMX uous 04 5 DEGC CONTIN CALCTD DAILY*MX uous CONTIN RCORDR uous 19 0.1 0.1 MG/L Five per CALCTD* MO AVG DAILYMX Week CONTIN CALCTD uous ~ --.,~ / / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under t direction or supervision in accordance with a system designed to assure that qualified Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inquiry oft e person or persons who manage the system, or those persons directly responsible for gathe ing the information, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used ~ S~ Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE DATE 423 843-7001 17 09 11 I AREA I NUMBER YEAR MO DAY CODE Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Na~-~~-SEQUO~~u~~ARPLANT ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) F - FINAL Form Approved. OMB No. 2040-0004 Addres_L _f.~BOX 2000 ___________ _ ___ -11.NTEROFFICE OPS-5N-SQNl _______ _ _ _ _ _§_ODDY - DAISY....INJ7364_ _______ _ Faclfily _ __IVA-_fil:QUOYAH NUCLEAR PLANT _____ _ Locatio.!L.J:!AMIL TOl:!_COUNTY_ _________ _ ATTN:Millicent Garland PARAMETER IX IC25 STATRE 7DAY CHR SAMPLE CERIODAPHNIA MEASUREMENT TRP38 1 0 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT IC25 STATRE 7DAY CHR SAMPLE PIMEPHALES MEASUREMENT TRP6C 1 0 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREME.NT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT TN0026450 From QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM 42.8 MINIMUM 42.8 MINIMUM RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE I xx I ***

NOTE: Read instructions before completini:i this form. QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL I~ D / / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law !hat !his document and all aUachmenls were prepared under my direction or supervision in accordance wilh a system designed lo assure Iha! qualified i( Anthony L. Williams personnel properly gather and evaluate lhe information submiUed. Based on my inquiry of Iha person or persons who manage the system, or those persons directly responsible for gathering !he information, !he information submiUed is, lo lhe bes! of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware Iha! !here are significant penalties for submilling false information, including !he possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used v ~. ~ ite Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE DATE 423 843-7001 17 09 11 I AREA I NUMBER YEAR MO DAY CODE Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Na~~2~-SEQUO~~UCLEARPLANT ___ _ Addres.L _.e..Q,_BOX 20Q.Q._ ___________ _ ___ _llNTEROFFICE OPS-5N-SQ.Nl _______ _ _ _ _ _§ODDY - DAISYJ.N_]73B1._ _______ _ Facl!i!Y_.JYA-SEQ110YAH NUCLEAR PLANT _____ _ Locati~.JiAMILTO~OUNTY._ _________ _ ATIN:Millicent Garland NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 118 G EAR From 17 ~-~--~--~ MAJOR (SUBR 01) F-FINAL WASTEWATER & STORM WATER EFFLUENT

- - NO DISCHARGE I xx I ***

Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT 19 00300 1 0 PERMIT 2 MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 19 00530 1 0 PERMIT 100 MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE M~ASUREMENT 25 00545 1 0 PERMIT 1 MUL ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX MONTH

FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 50050 1

0 PERMIT Req. Mon. Req. Mon. MGD ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MO AVG DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT Pl;RMIT ~ ) REQUIREMENT / / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law Jhat lhis document and all attachments were prepared under my / ~ TELEPHONE DATE direction or supervision in accordance with a system designed Jo assure that qualified g( Anthony L. Williams personnel properly galher and evaluate Jhe information submitted. Based on my inquiry of lhe person or persons who manage Jhe system, or those persons directly responsible for galhering ~ite Vice President Site Vice President r the information, the informalion submitted is, to the besl of my knowledge and belief, true, 423 843-7001 17 09 11 accurate, and complete. I am aware that lhere are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfaU. No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ _!VA~EQUOYA~UCLEARPLANT ___ _ Addres,L _E.~BOX 20QQ... ___________ _ ___ _(iNTEROFFICE OPS-5N-SQ.Nl _______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 T MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 ___ 20DDY-DAISY..lij73BL _______ _ BIOMONITORING FOR OUTFALL 101 Facl!i!Y_....I.VA-.§EQUOYAH NUCLEAR PLANT _____ _ LocatiOJL _!:!AMIL TQt!_COUNTL_ _________ _ EFFLUENT ATTN:Millicent Garland 31

- - NO DISCHARGE D...

NOTE: Read instructions before completinQ this form. DA PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE >100.0 0 3/180 COMPOS 23 CERIODAPHNIA MEASUREMENT TRP38 1 0 PERMIT 42.8 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE >100.0 0 3 / 180 COMPOS 23 PIMEPHALES MEASUREMENT TRP6C 1 0 PERMIT 42.8 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT 'PERMIT REQUIREMENT SAMP.LE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT ~ / / / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penalty of law that this document and all altachments were prepared under et ~idenf TELEPHONE DATE direction or supervision in accordance with a system designed to assure !hat qualified Anthony L. Williams personnel properly gather and evaluate the information submitted. Based on my inquiry of e person or persons who manage the system, or those persons directly responsible for galhe 'ng the information, the information submilled is, to the best of my knowledge and belief, true, 423 843-7001 17 09 07 Site Vice President accurate, and complete. I am aware !hat there are significant penalties for submilling false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including !he possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled July 23 - 28, 2017. The final report will be included in the August DMR. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

TENNESSEE VALLEY AUTHORITY. TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: August 11. 2017

1. Facility I Discharger: Sequoyah Nuclear Plant I TV A

. 2. County I State: Hamilton I Tennessee

3. NPDES Permit#: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant *

5. Design Flow (MGD): 1.579

6. Receiving Stream: Tennessee River CTRM 483.6)

7. lQlO: 3,491

8. Outfall Tested: 101

9. Dates Sampled: July 23 -28. 2017

10. Average Flow on Days Sampled (MGD): 1910, 1913, 1781

11. Pertinent Site Conditions: Production I operation data will be provided upon request.

12. Test Dates: July 25 -August 01, 2017

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia) *

15. Concentrations Tested(%):

Pimephales promelas: UV treated Outfall 101: 10.7. 2L4, 42.8. 85.6, 100 UV treated Intake: 100 Ceriodaphnia dubia: Non-treated Outfall 101: 10.7, 21.4; 42.8, 85.6, 100 Non-treated Intake: t 00

16. Permit Limit Endpoint(%): Outfall 101: ICii = 42.8%

17. Test Results: Outfall 101: Pimephales promelas: ICii > 100%

Ceriodaphnia dubia: ICii > 100% Page 1of93

18. Facility

Contact:

Millicent Garland Phone#: (423) 843-6714

19. Consulting I Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone#: (828) 350-9364

21. TVA

Contact:

Donald W. Snodgrass Phone#: (256) 386-2787

22. Notes: Exposures to samples collected July 23 -28, 2017 from Outfall 101 resulted in no toxic effects to fathead minnbws or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

j Page 2 of93

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall' 10 l, Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) .Temp. TRC* Time (ET) ID Collected Received (DC) - (m£?:/L) Last Used Bv I 101 07-23-17 0750 to 07-24-17 1235 2.1 <0.10 07-25-17 0926 07-24-17 0650 07-26-17 0827 Intake 07-23-17 0810 to 07-24-17 1235 0.6 <0.10 07-25-17 0926 07-24-17 0710 07-26-17 0827 101 07-25-17 0720 to 07-26-17 1232 2.6 <0.10 07.;27-17 0847 07-26-17 0620 07-28~17 0910 Intake 07-25-17 0735 to 07-26-17 1232 2.7 <0.10 07-27-17 0847 07-26-17 0635 07-28-17 0910 07-27-17 0735 to 07-29-17 0930 101 07-28-17 1234 1.7, l.9t <0.10 07-30-17 0900 07-28-17 0635 07-31-17 0908 07-27-17 0745 to 07-29-17 0930 Intake 07-28-17 0645 07-28-17. 1234 0.9 <0.10 07-30-17 0900 07-31-:17 0908

TRC = Total Residual Chlorme tsamples were collected in two 2.5 gallon cubitainers. Temperature wa.S measured in.each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were wam1.ed to test temperature Page3 of93 (25.0 +/- 1.0°C) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Pimephales promelas Test Organisms:

1. Source:

In-house Cultures

2. Age:

< 24~hours old. Test Method Summary:

1. Test Conditions:

Static. Renewal

2. Test Duration:

3. Control I Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

+/-

5. Organisms per Replicate: 10

6. Test Initiation: (Date/Time): 07-25-17 0835 ET

7. Test Termination: (Date/Time): 08-01-17 0800 ET

8. Test Temperature: Outfall 101:

Mean= 24.7°C C24.2-25.2°C)

9. Physical I Chemical Ceriodaphnia dubia In..:house Cultures

< 24-hours old Static. Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic lQ l 07-25-17 0926 ET 08-01-17 0830 ET Mean = 24.9°C (24.6 - 25.2°C) Measurements: Alkalinity. hardness. total residual chlorine, and conductivity were measured at the laboratory in each *100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Page4 of93. Statistics were performed according to methods prescribed by EPA using ToxCafo version 5.0 statistical software (Tidepool Scientific I Software, McKinneyville, CA).

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.* (Genus species)

(Type /Duration)*

Conducted July 25 - August 0 l, 2017 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used.:..:. davs) (%Effluent) 1 2 3 4 5 6 7

Control, 100 100 100 100 100 100 100 UV-treated 10.7%

100 100 100 too 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100. 85.6% 100 100 100 100 100 100 100 . 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100

Control, 100 100 100 100 100 100 98 Non-treated Test Solutions Mean Dry Weight (mg)

(%Effluent) (replicate number) 1 2 3 4 Mean

Control, 0.588

.0.556 0.528 0.602 0.569 UV-treated 10.7% 0.612 0.648 0.585 0.650' 0.624 21.4% 0.649 0;584 0.603 0.576 0.603 42.8% 0.664 0.609 0.659 0.655 0.647 85.6% 0.576 0.606 0.640. 0.569 0.598 100.0% 0.617' 0.570 0.568.. 0;540 0.574 Intake 0.530 0.592 *. 0.557 0.555 0.559

Control, 0.660 0.605 0.560 0.636 0.615 Non-treated IC25 Value: > 100%

Calculated TO Estimates: < 1.0 TUc* Permit Limit: 42.8% Permit Limit: 2.3 TUc

95% Confidence Limits:

. Upper Limit: NA* Lower Limit: NA

TUa = 100/LCso: TUc = 100/IC2s Page 5 of93

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a

Ceriodaphnia dubia Chronic/ 7-day ToxiCitv Test.

(Genus species)

(Type I Duration)

Conducted July 25,- August 01. 2017 using effluent from Outfall 101. Percent Surviving Test . (time interval used-days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100

.100 100 100*

. 100 100 10.7% 100 100 100

100.

100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (%Effluent) Data (replicate number) I 2 3 4 5 6 7

8.

9 10 Mean Control 28 30 27 30 29 32 30 31 30 30 29.7 I 10.7% 30 35 32 31 34 33 33 33 35 32 32.8 21.4% 32 38 33 30 34 35 35 34 35 34 34.0 42.8% 36 37 36 36 36 34 34 36 31 34 35.0 85.6% 36.37 35 38 38 37 35 36 32 38 .. 36.2 100.0% 40 38 38 38.34 39 36 41 37 38 37.9 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 42.8%

Permit Limit: 2.3 TUc 95% Confidence Limits:*. Upper Limit: NA Lower Limit: NA

TUa = 100/LCso: TUc = 100/ IC2s Page 6 of93

TOXICITY TEST RE SUL TS (see Appendix C for Bench Sheets).

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted July 25 -August 01. 2017 using water from Intake Percent Surviving *. Test (time intenral used.c.. days) Solutions 1

2 3

4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100. 100 100 100 100 Test Solutions Reproduction: (#young/female/7 days) (%Effluent) Data (replicate number) l 2 3 4 5 6 7 8 9 10 Mean Control 30 28 30 27 30 29

30..-29 29 31 29.3 Intake 32 38 35 32

'35 31 29.34 32 34 33.2 IC2s Value: > 100% Calculated TU Estimates: <LO TUc* Permit Limit: NI A Permit Limit: N/ A 95% Confidence Limits:* Upper Limit: NA Lower Limit: *NA

TUa = 100/LCso: TUc =.100IIC2.s.

. 1*-:. '. REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) *

Species Date Time.* *Duration Toxicant Results (IC2s)

Pimephales promelas July 11 - 18, 2017 0810

7. days KCl 0.75 g/L Ceriodaphnia dubia July 11-18, 2017

.. 0915 7-days NaCl 1.06 g/L Page 7 of93

~ PHYSICAUCHEMICAL

SUMMARY

CQ Water Chemi'ltry Mean Values and Ranges fur UV-treated Pimephales promelas and Non:treated Ceriodaphnia dubia, Sequoyah Nuclear Plant (SQN), EfHuent Outfull IO 1 and Intake CD perfunnidJuly25-August01, 2017. co 0.... cc CA) Test Sample ID Temnerature ("C) Dissolved Oxygen (mg/L) Initial Final Initial Final . Control, 24.7 24.5 7.7 7.4 Non-treated 24.6 - 24.8 24.3 - 24.7 7.6 - 7.8 7.0 - 8.1

Control, 24.8 24.6 7.8 7.3 UV-treated 24.7 - 24.9 24.3 - 25.0 7.6 - 8.0 7.0 - 7.8

.s 10.7% 24.9 24.5 7.9 7.2 ii 24.8 - 25.0 24.2 - 24.8 7.6 - 8.0 6.8 - 7.8 ~ 21.4% 24.9 24.5 7.9 7.2 c:i. 24.8 - 25.0 24.2 - 24.8 7.6 - 8.0 6.7 - 7.8 -l!l 24.9 24.5 7.9 7.1 42.8%. 24.4 - 24.7 l 24.8 - 25. l 7.6 -. 8.1 6.6 - 7.9 85.6% 25.0 24.6 8.0 6.9 Q, 24.9 - 25.1 24.4 - 24.7 7.6 - 8.1 5.7 - 8.0 100% 25.0 24.6 8.0 6.7 24.9 - 25.1 24.3 - 24.8 7.7 - 8.2 5.7 - 8.0 Intake 25.0 24.6 8.0 7.1 24.7 - 25.2 -24.5 - 24.8 7.7 - 8.2 6.5 - 8.0

Control, 24.7 25.0 7.7 7.8 Non-treated 24.6 - 24.8 24.8 - 25.2 7.6 - 7.8 7.6 - 8.0 10.7%

24.8 24.9 7.6 7.8 24.6 - 24.9 24.7 - 25.2 7.6 - 7.7 7.6 - 8.0 .s 24.8 24.9 7.7 7.8 21.4% .ij 24.6 - 25.0 24.7 - 25.2 7.6 - 7.8 7.6 - 8.0 .s 24.8 24.9 7.7 7.8 42.8% .:: er 24.6 - 25.0 24.7 - 25.1 7.6 - 7.9 7.6 - 8.0 24.8 24.9 7:7 7.8

~

85.6% a 24.7 - 25.0 24.8 - 25.2 7.6 - 7.9 7.6 - 8.0 100% 24.9 24.9 7.8 7.9 24.7 - 25.1 24.8 - 25.l 7.6 - 8.0 7.7 - 8.1 Intake 24.9 25.0 7.8 7.9 24.6 - 25.1 24.9 - 25.1 7.5 - 8.1 7.7 - 8.1

Note: Total residual chlorine was perfonned on non-treated Outfall 101 and Intake samples.

Overall temperature ("C) Pimephales promelas Ceriodaphnia dubia Average 24.7 24.9 Minimum 24.2 24.6 Maximum 25.2 25.2 pH(S.U.) Conductance Initial Final (µmhos/cm) 7.94 7.61 317 7.77 - 8.11 7.46 - 7.88 308 - 324 7.94 7.61 310 7.86 - 8.03 7.48 - 7.87 301 - 315 7.94 7.57 303 7.86 - 8.03 7.39 - 7.83 294 - 310 7.95 7.58 291 7.87 - 8.04 7.40 - 7.83 282 - 300 7.95 7.56 264 7.87 - 8.05 7.43 - 7.88 257 - 210 7.95 7.49 213 7.87 - 8.05 7.32 - 7.85 206 - 216 7.93 7.47 195 7.81 - 8.05 7.30 - 7.85 189 - 203 7.94 7.54 191 7.84 - 8.05 7.36 - 7.83 185 - 195 7.94 7.91 317 7.77 - 8.11 7.83 - 7.99 308 - 324 7.96 7.89 303 7.84 - 8.10 7.80 - 7.97 292 - 317 7.96 7.89 295 7.84 - 8.10 7.79 - 7.97 280 - 304 7.96 7.88 267 7.85 - 8.11 7.78 - 7.95 254 - 272 7.95 7.87 213 7.85 - 8.10 7.77 - 7.95 207 - 218 7.94 . 7.86 196 7.86 - 8:08 7.76 - 7.95 190 - 201 7.95 7.91 192 7.86 - 8.09 7.79 - 8.01 187 - 196 Alkalinity Hardness

Total Residual (mg/L CaC03) (mg/L CaC03)

Chlorine (mg/L) 59 86 58 - 61 85 - 87 59 84 58 - 59 83 - 87 70 76 <0.10 69 - 71 73 - 77 <0.10 - <0.10 71 80 .<0.10 71 - 71 73 - 89 <0.10 - . <0.10 59 86 58 - 61 85 - 87 70 76 <0.10 69 - 71 73 - 77 <0.10. - <0.10 70 80 <0.10 69 - 71 73 - 89 <0.10 - <0.10

SUMMARY

I CONCLUSIONS Exposures to samples collected July 23 - 28, 2017 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Page 9 of93

Appendix A ADDITIONAL TOXICITY TEST INFORMATION *

SUMMARY

OF METHODS 1.. Pimephales proinelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastiC disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels co,nsisted of 30-mL

. polypropylene cups, each containing 15-mL oftest solution. DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas

Samples used in the fathead minnow test were exposed to UV light for two minutes prior to introduction of test organisms. UV treatment is used to control interference of fish pathogens. This treatment method was approved on November 23, 2015 by the State of
Tennessee in *a letter from Jessica Murphy to Terry Cheek, Senior Manager of TV A Water Permits, Compliance, and Monitoring.

2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

2. Ceriodaphnia dubia None Page 10 of93

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pert~ining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B-2010.

4. Dissolved oxygen was measured by SM 4500-0 G-2011.

5. The pH was measured by SM 4500-H+ B-2011.

6. Conductance was measured by SM 2510 B-2011.

7. Alkalinity was measured by SM 2320 B".2011.

8. Total hardness was measured by SM 2340 C-2011.

9. Total residual chlorine was measured by ORION 97-70-1977.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCL

2.

Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses. Page 11of93

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEP A. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 2012.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 12of93

Page 13of93 Sequoyah Nuclear Plant Biomonitoring July 25 -August 01, 2017 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser pischarge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling

. Table B-L Sequoyah Nuclear Plant Outfall IOI Diffuser Discharge Concen,t~atioris bf Chemicals Used to Control Microbiologically Induced Corrosion and. Mq~lusks, During Toxicity Test Sampling, Date .. 'Sbdiutn*. Hypochlo~ie

.. :.'.;,~*-~:

02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/1112005 06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10i2005 07117/2005 07/18/2005 07119/2005 07/20/2005 07/2112005 07/22/2005 10/30/2005 10/31/2005 1110112005 11/02/2005 11/03/2005 11104/2005 ',*.J' 11114/2005 11/15/2005 11116/2005 11/17/2005 11/18/2005 11119/2005 "... - Page 14 of93 Towerbrom lngJL TRC <0.0042 <0.0116 <0.0080 0.0199 <0.0042 0.0155 0.0063 '0.0043 0.0103 0.0295 0.0129 0.0184 0.0109 0.0150 0.0163 0.0209 0.0242 0.0238 0.0068 0.0112 0.0104 0.0104 0.0117 0.0165 0.0274 0.0256 0.0234 0.0231 0.0200 0.0116 Februafy 6; 2005 -July 28i 2017 PCL-222:

PCL-401 CJ;.,-363 Cuprostat-PF H.::BoM

.

iiiWt: * *..
mgJL mgJL mgJL mWL..

':i>ijosphate'~ Copolymer <nM:AD> Azole Quat

.... 0:028*~~

0.028**

1<
0:028 <*

" 0*:048:. 0.028 ., Q,028._. .~;*-: */ 0:026 0;1)26.;

Q,026

'0.02.6

0:.026'"

. 0:0.?4~. ;> ~ ,*':*:r* 0.010 0.010 0.010 0.010 o:cno 0.010 0.009 0.009 0.009 0.009 0.009 0.018 Nalco 73551 mg/L EO/PO 0.007. 0.007 0.014 0.014 'H:,150M mg/L *. . :QWit:. r' -*-- ,0.037-. 0.037*.. . o.p~7 Q.036 0.036 0.036

0.035. -*
0:036."<

0;036.* _ o,.m5*.

Date 11112/2006 11113/2006 11114/2006 11115/2006 11116/2006 11/17/2006 11126/2006 11127/2006 11/28/2006 11/29/2006 11130/2006 12/0112006 05/28/07 05/29/07 05/30/07 05/31107 06/01107 06/02/07 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/13/08 04/14/08 04/15/08 04/16/08 04/17/08 04/18/08 10/26/08 10/27/08 10/28/08 10/29/08 10/30/08 10/31108 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and 'Mdllusks, During Toxicity*Test Sampling, February 6, 2005 -July 28, 2017 Sodium Towerbrorn Hyll_ochl?rite mg/L

.,mg!L.

TRC . TRC , "\\ ,1, 0.0055 0.0068 0.0143 0.0068 0.0267 0.0222 0.0188 0.0138 0.0120 0.0288 0.0376 0.0187 0.0084 0.0103 0.0164 0.0305 0.0241 0.0128 0.0238 0.0158 0.0162 0.0175 0.0039 0.0124 . 0.0229 0.0143 0.0120 0.0149 PCL-222

mgtL-*.

Phosphate I t* '"' /;'... ~* 0.0260 0.0151 0.0172 0.0154 le : 0~0086 Page 15 of93 PCL-401 mg/L Copolymer CL-363 mg/L *'. DMAD. j I* ~-**.~: '.1 A [" "£* Cuprostat-PF H"BOM mg/L mg/L.: Azole Quat

1,'

Nalco 73551' mg/L EO/PO. 0.017 0.017 0.017 0.018 H-150M* nig/L Quilt

0,037, ;

. 0.037*. .* 0.037 0.037 . I. -*~*. \\., '

o:'o'36* **

0.036 . '0.036 0.036 ' f ' 0.041 0.0,~l : 0.941 ~' 0.0~.1.. MSW 101 mg/L Phosphate 0.015 0.015 0.015 0.015 0.015 O.Q15 0.030 0.030 0.030

Date 02/08/09 02109109 02/10/09. 02/11/09 02/12/09 02/13/09 05/10/09 05/11/09 05/12/09 05/13/09 05114109 05/15/09 11/15/09 11/16/09 11/17/09 Table B-1. Sequoyah Nuclear Plant Outfall IOl

Diffuser Discharge C~ncerttrations of Chemicals Used to Control MicrobiologicaHy Induced Corrosion and Mollusks,.During Toxicity Test Sampling,

*February 6, 2005 -July 28, 2017

, Sodiu~ 1. Towerbrom.P.CL-. PCL-401 ~~~36_3., Cuprostat H-1$0.fy.1 Hypochlonte mg/L ' i2i~~ mg/L . riJ.glL' < -PF mg/L

ing/lf *.
mg/L* *
TRC mg!C'. Copolymer DMAD Azole

'*Quat'.: .TRC~: Phospll:* ate_,.* 0.0197 ~ ~- . 0.0237 0.. 0104 0.0155 0.0106 ~

, 'H*

,~. 0.0129 0.0415 "~. 0.0053 0.0049 I <o'.0141 ' ~ 1' <0.0160 0.025

- -..,c.

0.0152 ~ 0.0255

Nalco 73551 mg/L EOIPO 0.017

. 0.017

0.021 0.017 0.017

. Specfills. * .

CJ:l300 *.

mgJL**. " Qilaf.: 11/18/09 - - ~: '; 0.0306 ~ 11/19/09 0.0204 11/20/09 0.0093 05/09/10 0.0192 05/10/10 0.0055 05/11/10 0.0100 05/12/10 0.0171 05/13/10 0.0041 05/14/10 0.0099 Page 16 of 93 * ~ .. ~~.. ,'"' ~ :*: : : ,',q_ "/. 'J o-.<i39 ~,: 0.039 0.039 0.039 H-150M mg/L Quat 0.0446 0.0396 0.0396. 0.0397 . M~W. !: ':JOI:. 'irig!L. *' Phosphate

~..

,.'*I.

Table B-1. Sequoyah Nuclear Plant Outfall 101 . Diffuser Discharge Concentratfotis 1of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Te~t Sampling, February 6, 2005 - JUiy 28, 2017 Date

Sodium Towerbro PCL-401.3qt::3.6?.. Cuprostat ff:J,3~]\\.1 Nalco.

73551 mg/L Sp~ctrlis; H-150M *MSW Floguard

CT1300
mg/L. ?J.0,(.*. MS6236 10/31/10 11/01/10 11/02/10 11/03/10 11/04/10 11/05/10 05/01/2011 05/02/2011 05/03/2011.

05/04/2011. 05/05/2011 05/06/2011 11/06/2011 11/07/2011 11/08/2011 11/09/2011 11/10/2011. 11/11/2011 : 05/06/2012 05/07/2012 05/08/2012 05/09/2012. 05/10/2012 05/11/2012 08/12/2012 08/13/2012 08/14/2012 08/15/2012 08/16/2012 08/17/2012 05/12/2013 05/13/2013 05/14/2013 05/15/2013 05/16/2013 05/17/2013 09/15/2013 09/16/2013 09/17/2013. 09/18/2013 09/19/2013 09/20/2013.

Hypo-mg/L chl&i-ite.

TRC

mgffi

me':

Page 17 of93 ~22: mg/L

- - *~g/B,, -PF mg/L.;tngl,L,~,;

. rilg/L.:: Copoly-p,~:, Azole

- iQuaf:~:

Phos.:.. mer EO/PO

- hate.*

Quat *

illg;L mg/L

~~ps+; Phosphate

. hate 0.029 0.029 0.029 0.029 0.029 0.032 0.064 0.06'4 O.OM 0.032 0.032 0.03 0.03 0.03 0.03 0.03 0.03

~-------- ----- --

Table B-1. Sequoyah Nuclear Plant Outfall IOI /Diffuser Discharge Cbncentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, Date S,odium Towerbro

- ayp~;:

mg/L 05/04/2014 05/05/2014 05/06/2014 05/07/2014 05/08/2014 05/09/2014 09/07/2014 09/08/2014 09/09/2014 09/10/2014 ' 09/11/2014 09/12/2014 08/09/2015 08/10/2015 *. 08/11/2015. 08/12/2015. 08/13/2015 08/14/2015 10/18/2015 10/19/2015 10/20/2015 10/21/2015. 10/22/2015. *. 10/23/2015 05/15/2016 05/16/2016 05/17/2016 05/18/2016 05/19/2016 05/20/2016 07/31/2016 08/01/2016 08/02/2016 08/03/2016 08/04/2016 08/05/2016 04/30/2017, 05/01/2017 05/02/2017 05/03/2017 ' 05/04/2017 05/05/2017 chlorite~ TRC.

'fu,g/L*

me 0.0118 0.0112 0.0096 0.0164 0.0235 0.0110 0.0070 0.0074 Page 18 of93

. February 6, 2005-July 28, 2017
'.<;~~3.p3'..; Cuprostat JEt3.0M

'* nigi'E:*,< -PF mg/L t,1mg!L *.. ... r>~... Azole ,.)~u~f,/ ,~~ I '" r M~W: Floguard .ror.: Ms6236 . 'tng/1,2 '. mg/L .. 'i>ijo~.:: Phosphate hate~ 0.03 0.03 0.03

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations bf Chemicals Used. to Control Microbiologic'ally Induced Corrosion and MolluskS, During Toxidty Test Sampling, February 6, 2005 -July 28, 2017 Date i Towerbrom* Flo guard Spectriis BD 1500 Nalco Spectrus mg/L MS 6237 mg/L 73551 . CT1300 TRC mg/L

Quat mg/L

., nig/L Phosphate/Zinc EO/PO Quat **

.. i 07/23/2017 07/24/2017 0.0124 0.04/0.01 1 07/25/2017 0.0081 0.04/0.01 0.0232 07/26/2017 0.04/0:01 .. ':0~0'3." :, ' 07/27/2017

0.0179 0.0296.,

0.03~ 07/28/2017 Page 19 of93 Flo guard MS6236 mg/L -Phosphate

Page 20 of93 Sequoyah Nuclear Plant Biomonitoring July 25-August 01, 2017 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

~ent: TVA ~ject Name: Sequoyah NP Toxicity ~*Number: NIA

nciiity Sampled: SeqLloyah NP NP DES Nu111ber: TN0026450 BIOMONITORJNG CHAIN OF CUSTODY RECORD Page _1_ of_l_

Environmental Testing Solution, Inc. 351 Depot Street. Phone: Fax: Asheville, NC 28801 828-3 50-93'64 828~350-9368 Delivered By (Circle One):*. FedEx UPS Bus* Client Other (specify): :Son; Q.. Pe.\\;\\/~ I y Sumplc Description Field !dcnfffication I 0'mb/Comp "" Collection Datemmc. Container Number 8{, Volume Collected Flow (MGI?) Rain Event? SQN-101-TOX SQN-INT-TOX Comp Start ~-23*17 o7So ~ Comp 7*7.:J.-/7 oY/6 ~ Relinquished By (Signature): (Mark as Appropriate) Yes End I (2.5gal) Tf¥es, Inches o.J1 7.. Jl/47, l (2.5 gal). . NA 'x.... _ 7 p? /.!> ff..i:' /)* 3 Sample Custody - Fili ln From Top Down No Tr;1ce P.T~n.og Nuniber \\~1J11'f.b I l'to;ci~.02... Jf.. c..u~ '"t'()"b,"-\\ Ariivill 'fe111p. Oy Time Appear* ("C) 'E..-r

ancc,

'2,\\'C O' '""'"!f:> f..

().IQ"(_ 't'- ', ,~<., ~ Se.t>..~ '~~(.,'~ $p...\\-\\1"..5 *~1,JeJ) Date/Time Received By (Signature): Ul~~\\\\10N. \\RLA~':.~~ I..., Pl.LL Date/Time 'ii At,\\:'1~. IA .Sow\\ c.. ... - **. K\\ '"-..../ Instructions: Cli\\~nt!l should fill ill all nrcns except those in the.. l.ahoratory lJge'" bli:1ck. Biomonitoring samples 11rc prese!'Vcd by stod11g them at 6C and shipping them in ice. The hnld ti111c for each sampli: i:; 36 hours frnm the ti111c of collection. Therefore, plcnsc collect and ship ir'< such n wny that the luboratory will receive rhc smnplcs \\\\'ith ample linw to initii1le testing within that rime* frame. Samples shipped overnlghl on Fl'ida}; via FedEx or*UPS r'rlusl he mnrked lor Saturday delivery tir lhcy w'ill m1l arrive until the lbllowing Munday.

) Whole Effluent Sample Receipt Log Page~ 'Sample cemper~ture performed usine Sample Rec_.;IYln~ Thermometer: SN 160928522 Date Time Received Received "Sample Project Sample Sample name and description State Comments Recehieu Received by 'irom 1emp. \\ 0.q nt1mher number 07;24.17 1235 J. Sumner TVA Courier

1..1 12585 170724.01 TVA-Sequoyah Nuclear.Plant-101 TN.

07-2{1*17 1235 t Sumner. TVA.Courier 0.6 12585 17072.4.02. TVA* Sequoyah Nuclear Plant-Intake TN SOP G4 -1!.-<hlblt <;4.2, revision 01-03-J2

BIOMON1TORING CHAIN OF CUSTODY RECORD Page._1_ of l ~en!: TVA Environmental Te~ting Solution;, Inc.

Delivered By (Circle One):

~ ~ject Name: Sequoyah NP ToxiCity 3 51 Depot Street. FedEx UPS Bus Clieni Asheville, NC Other (specify): SDn,~(I Ve\\ i' vef t/ ~*Numb.en N/ A ~ Generaf'Comme~: @. 715 28801 r:acility Sampled: Sequoyah NP 7¢/C ~j>IV:t -t'd,.., l~I,j.,.:;f~ c; NPDES Number: TN002645Q Phone: 828-350-9364 /;cl! Sa.... plt!J./("()m.:M /. e ~7/ey,£ 1)77/0 Collected By: Mar~ o. Maorc. bc-)"flor. n. Fax: 828-3~0--9368

/~.IL_

h I..

~\\)iss. Me.to!~ ~ue CoUe("#+ed ¥ 1'.. ~4 D'rl '61-k

.,,J_ Field lde0titica1i_on I Grob/Comp r-p!Wjuf~ (258S r Collection Datcffime Container Flow Sample Descriprion Nuinbcr & (MGi:)) Rain Event? Laboratory Use. Volume (Mark as Appropriate) Collected Yes

lfVes, No*

Trace ETS Log Atiival Temp, 8}' lm1c J\\ppear-Inches Number ("C) nnce Start End E.."t'" SQN-IOl~TOX Comp 7-JS:l'-1 ?'-~*1'7 1 (2.Sgal) x \\1t>1-'2.ho.0\\ 'i' \\,_:,'t-J<//~.~n ?.....~"{. ~ 1J'1Zo ~r

ohi.o rr SQN-INT-TQX

--.Cotnp 7.. 3'5*1'7 7*ZIP-/7

1 (25 gal)

NA x \\ ~':\\l.b.07. It ~

073-fJ'. E.,-

/')/l'j rfi' ff .. L1. t_ \\1) Sample (Justody-Fill In from Top Down ~ ~'t ~\\.S I~\\. ~l\\Wl\\.6 l\\C:a(f\\~ ) It..> &l:ob CO~b\\\\ION. n'\\.(.. "'~ ' V.J N...'-- Relinquished Sy (Signature): Daw/Time Received By (Signature): - Daterrime,9'~e-s 'dLl~/7i//1 7,21:;--;7/~!11-E:\\ ~;f!f~ St>~H- -\\.I 0 ?-:l.6-1/

()8:fr E.T

(/ ~.-Oa_ sf)~u:.. I i.., t\\S ra (( {J>i-lb* I j It ~31.. ~r\\wt--- o1-1k *Ii r'2~7_J.\\ I 'J ~ ~ \\nstrnctions: Cllen\\s*shmi\\d fill in \\\\ll arca;.exc.:c:pl those in lhc "Laboratory Use" block, Biomonitoring samplr!s are preserved by storing them al 6"C 1i.nd shipping lhcm in ice. The hold time for each sample is 3(i liour~ from the tiinc of collection. Therefore. plc:1sc collect and ship in such a way that the laboratory will receive the i;amplcs with <lmplc time to initiate icsting i.vithin that time frnme. Satuples shipped twcrnighl on Friday via FedF.'.x or UPS 111u:;t be m3rkcd for Saturday delivery or they will t\\01 arrive unril the following Monday.

Whole. Effluent.Sample Receipt Log i>age qz_,

sample temperature pcrrorrne~ using Sample fiecelirlng illennometer: SN 160928622 Date TI me Received Received
Sample f'roject
Sample Sample name and description State Comments Received Recefved by from Temp. (°C) number numb.er 07-26,17 1232 J. Sumner TVA Courier 2.6 12585 '

170726.01 WA - Sequoyah Nuclear Plant- :101 TN 07-26.-17 1232' J. Sumner TVA Courier '2.7 12585 170.72.6 :02 TYA-Sequoyah Nuclear Plant*. intake.. TN SOP Gtl - Exhibit Gll.2, revision OJ,-03--li

BIOMONITORING CHAIN OF CUSTODY RECORD Page_l __ of 1 C"i)ent' TV A Environmental Testing Solution, Inc. Dellvered By (Girc\\e One): -~ Jt&,ject Name: Sequoyah NP Toxicity 3 51 Depot S tteet. FedEx BPS Bus Client ~. P~. Number: N/ A Asheville, NC Other (specify);.:Sn(\\\\.<!.. De-\\i vef.V I <o . General. Comments* . ~ llatility Sampled: Sequoyah NP 2880l TooK Sa;~~b f'rc...., \\ti\\ ~a..w;~l'er@._ lJ7.5(..) r-6".- NPDES Number: TN0026450 Phone: 828-350-9364 tot>~ f::r;....,... _le.'.ft,o ~ ~e... ~~l-er@. v 7.._...,-.::;, Colloored B~?

,: :;;x, Fai:

828-350-9368

tO<s.s. i A.,,J.cJs,..:o,.:.r*c tJr.11~ du/ + W4 ~" ~ik l1ield tdcntificatloo /

Grab/Comp / Collcctioli Dactffime Container Flow pn,oj v:t ll t~* Sample Description Number& (MGO) Rain Event? Laboratory Use Volnmc (MarK as Appropriate) Collected Yes If Yes, No . Trace ETS Lng Arrival Temp: By 'Time Appear-Inches Numb1:_r (°C) an cc .Start End ~ SQN-1()1-TOX Comp <" r;-;z'l-17 1-zg.;7 2 (2.5gal) \\:ro~28.Dl ~ \\'\\"':>-\\ 11?1.11 x l). 9'-/ \\.:'\\ \\.'\\*it t . tJ.73~ !1 /J&J6 1;7' SQN-INTcTQX Comp '/'Z'/*1'7

7,zF--17 I (2.5gal)

NA \\-:\\-o1'Z.O.02.. ' o.Oi.*l ~ \\\\..".>..\\ {) 1~5 ~'!'" ~IPl./S' ~r x ().!ft/ ~ . Sample Custody-' Fill Tn Frpm Top Down

ff. l-JS-m'tt\\ ~'-5 \\W'\\N.,\\. SAM~

~Lf,\\\\I~ Relinquished By (Signature); Date/Tiine Received By (Signature}: \\;..) GOOb ~b\\'\\IQN, \\1tl{_ I\\~~ OatefTime l~ "-U- _M ~.A -4'.ol6 /LP~/71//~ '7--Z?-17/1J31D I ~'(j iZ ~

r.

Sei\\oool\\(.. d 'l-;:1. 'lf-1*1 /oB:*w ~~ -// ~ I '?JA A' .S0N\\C '*J!0--1? / 12: ~o/ ~r~l ~~ (J'Ylg-\\~ \\ ""L~... ~ ~T .,rw ~--;- __, I v

v*

lns1ructinns;* Clients should fill in all area~ cxc.:pt 1ho:;e in the l.nbornlory lise" block. B101110nitoring ~amples nrc prcscrvctl by storing then1 al. 6"C and shipping them in ict:. The hold time rt1r <:adi ~ample is 36 hours lh1m the time (If c0\\tccll<1n. Tl1crc.fl\\n!. plea~~ collctl m'1d,;hip in sud\\ 11 w11y Iha\\ lhc lahornlmy will receive \\he snmplc;; with ample 1i1nc lo iniliatc le.sling wilhi111h:11 ti1ne thune. Smtipks shipped overnight on Friday via FedEx or UPS must be market! for gaturday delivery or they will not arrive until the following Monday,

Whole Effluent Sample Receipt Log 'Sample temperntu~ performed using Sample l\\eceMnr. Thermometer: SN 160928622 Date 1irile Receive*d Received "'"Sample Project Sample Sample name and description State Comments Rect;ived R_eceived by from* Temp *. ( 0C) number number 07~28-17 1234 J, Sumner TVA Courier. 1.7/1.9* 12585 170728.OJ TVA-Sequoyah Nuclear Plant* 101 TN 07.-28;17' 1234 J.Sumner. TVA Courier 0.9 125SS 170728.02 TVA* Sequoyah Nucle?.r Plant-Intake* . TN SOP G11 - Exhibit G4.2, revision 01-cl3~n

l I I' Page I l1f7 Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013 Method 1000.0) Speci¢s: Pimeplwles.promelas CHent: Tennessee Vallev Authorit;* Facility: Sequoyah Nuclear Plant NPDES #: TN0026450 Pro,iect#:

\\""l..SRS

.Dil11tio11 vrepamtio11 information: Dilution prep(%) 10.7 2L4 Effiucnt volume (rnL) 267;5 535 42.S* 1070

Diluent voluinc: (niLJ 2232.5 1965 1430 Totalvolmnc (ml) 2500 2500 2500 Test organis111,i11formatio11:

Organ)sm source: In-house culture Age: < 24-llours old Spawn date: O"l*\\C\\* n 85.6* 21'10 360 2500 Hatch dates,and times: Oi*-1.'-\\* t'"l \\'oC::.O ~ IOO 2500 0 2500 <i"\\.,"l.S

n 6\\o~

Transfer vesi;elinfonnation: pH= .,.'°\\'"\\ s,u_ Temperature*= "'U\\*'.1;.. oc

. Average transfer volume:

<0.25 mL Dail)'feedilzg a11d re11ew11l i11Jormntio11: County: Hamilton Outfall: 101 Comments:. Each concentration was UV-treated for2 minutes.to remove pathogenic* . Interferences. 1'esr i11formaiio11: . Randomizing template: UErtlr OMN~ lncubator number and 1 e., shelf location: Arremia CI*fM numbitr: CHM914 JJryi11g iliformaliollfor weight delermi11a1io11: Date I Time in.oven: ofMt~-s"\\ rJeic; IIiitial oven temperature: lah' ( Date I Time out of oven: 1'.i-01.*ti OSie Final oven temperature:: \\tit!.(.,, Total drying time: '1.. '.\\.~ llb.J.Q s. Dn_y Date Morning feeding Afternoon feeding. Tcsl hJitintiun,. Snmplc numbers used i\\'11-JSW renewal, or batch termination used Time Analyst* Time Analyst Time Annlyst Outfnll 101 lntnkc 0 07-25-17 .()t.~S. ~\\ \\..,_J.o ~{ .M~?f:. \\1 '1.<!'11.'-\\.o \\ nm.."£:\\. o1-Ul*lS*J1. I 07:..26~11 oroos \\( n.'40. J( 0 lO<i \\.1. l'""\\ 0'\\1.'-l-ti \\ l\\C'11.'-\\ *01. tr\\-\\'i* \\1 2 07~27~17 Olci~~ H \\"1..LtC. v \\A os.l.\\'1 \\101'1.lo.() \\ ,-un *u ** en. 01-~'1*,, 3 01-28-11 oc.. \\a '\\t (),,lC \\l \\~10 l.i a\\!_.C.,, 0 \\ "\\ (j"\\ 1.,\\.

0\\... en* L"\\"'n

.! l\\a"\\1.~' 0 \\. t'\\{j\\~~- 01_ 01-1.."\\. '1 6 07-31..:j7 O"\\CI~ }(. lbC>S. ., tl l~~,iJ ~ lf 11 '\\ 1.-<> \\ ~10'11.t*OZ... 01"1...,*11 7 " *!?'"* '~:'" fl 08-01-17 I o~ro, v Conlwl infonuatii:m:

\\)'1-'Ta:er-.~~b Acccplnnce cri,tcria Summary oftest eniluoints:

% Mortality:
61.
s20% 7-day LCso > (()\\)'1_ Average weight pe" initial larvae: o.9ot\\ NOEC lOC1. Average weight per surviving larvae: <:i.s~~ > 0.25mgllarvnc LOEC 'IC\\f\\1 ChV '";) rnc1.
IC2s
>te-07. Page 27 of93 SOP AT20*-Exhibit AT20.3, revision 11-01-14
i I .}. 1~""' Page2 of7 Species: Pimepluiles promelas Client: TV AI.Sequoyah Nuclear-Pfo11t Outfall 101. UV~treated Date: 07-25-17 Survival mul Growth Data Day CONTROL 10,73 21A%. A B c D E ' .F G H
1 J
K L 0 lO ID .JO J'O JO JO 10 10 ID ro JO JO l 10 (0 lo {C) JO lo to 10 10 ID 10 10 2 lO IC l \\'.) to lo: lO 10 to lQ. f D to I() 3 10 to io l l) tD f.0 LO to lO co IO 10 4 ID 10 10. l C>> 10 IQ to 10 l () IQ co ( b 5 to 10 lb ID ID ro ro IQ IO lO 10 10 6 lO 10 co /,0 IO 10 tO IQ LO ro £Q I t.i 7 10 10 IO lO I\\) JO LO IO 10 ID IQ t () . A =Pan weight (mg) Trnycolarcode::, ~ ~\\sl~\\.. \\'9.!9 "\\'¢- r.,c0 'b~
~~ 6~~ br:::;;\\
~r;._,O.. \\~~~"! \\\\,g,~ Analyst: 'ldtYv1 * ,~~~ Date: QJ1~!:\\-l"l ~~,. b* \\\\9" ~ ' "i * ~.. 8 = 1":111 + Ln~c wc!gbt.(mg) Analyst: Onte: aa*<r'::i*\\~ "'Z,\\."A "l.\\.lo"\\ '2.ti.CQ 1.\\.aC\\ '1.\\."\\li '"lo"l*it\\ '2.\\,'\\!> '2\\.~ -z\\~~o -u.~ C.'1.o5 "'tt.~ C= Larvae i1*cigbt(mg}"' B-A . !land c;1lculated. ~ s.&~ 'S.0:.1.a s,:1.~ b,o"L C...\\~ ~.u.g ~;as !,.So I;..~'!\\ -;,?.&.\\ c.o.~ s,"ile. A11:1lyst: Weigh I 11cr in Hin I number of lnr.vnc (mg) = C / lnilinl uumbcr oflnrvnc ~~" ~
~
t!;'"' (\\,.([,.
~
~* ~~ (,;, 'O.
,..°"-
c,l;;
\\:><?. "' llanll calc11h1lcd. (\\ &Ii 4
~

~
\\Q o* '.:S" Analyst: ~ o* t; o* Ci o* , o~ c ~* o' f;). '()* Avcr:1ge weight per rcr.ccnl rctluclion. ~*: - ~.... ~:1~ *- - ~ initin! number of from control(%) 0. sto'\\ i' ~ ' J 0; "1 '1..'-\\ - '1.'17. ().loo:!> -lo.\\ 7. j lnrvuc (mg) . !:--~~-----*.:. -~~*._,. Comment codes:.c =clear, d = dead, fg = fungus, k = ki lle9, m = missing, sk =sick, sm = unusually small, lg= unusually large, d&r= decanted and retu1i1ed, w = w!lunded. Co111me11ts: Page 28 of93 SOP AT20- Exhibit AT20.J, revision l 1-0J-:J4
l l I l -j _J Page 3 c*f7 Spceies: Pimephnles promelns Client: TVA/ Sequoyah NuclearPJ:mt. Outfall 101. UV-treated Date: 07-25-17 S1irvival amt Growth D11ta Day 42.8% 85.6% JOO% M N 0 p Q R s T u v w x O* JD JO JO JO JO JO lO 10 10 10 JO IO l (0. lO lO ~o lQ !O l ti IQ lO IQ IO 10 2 I 0
10 10 IG to lO to f O I{) <o to ro 3
\\0 10 /0 t*o LO [0 It) (CJ IQ tO ID '0 4 ID ' t> IC lb to 10" l t> I~ c () \\ C> t (J i () 5 l l) ( (} .to l tJ l() lO <o /0 l{J ( tl IQ l(J 6 lQ '{) lQ ' l () rt> rD l fi IG c {J 10 I\\) ft) 7 I \\J lD 'lo. I () /Q ff:) I~ , ti I ti I() I \\J 'D A= Pnn wciglil (mg) Trny color tollc::: ~_r,k ~~°' '¢.\\).; \\\\9,ry\\) ~Cb ~""" ~\\. '*"' ~() p.._ "'\\°'- ~'fr fJ~Lb .Analyst:*~ \\\\..\\~p Daic: ~"1" ~.. \\) ~* \\\\.9 *. ' ~. t>* ,\\,\\. f>* ~.
B =Pan -r L:i1\\ might (ing)
"?.\\.i~ Annlyst; Date: o&-~*\\1 ~\\.~~ '11.*SC\\,.~ *. \\~ -wf\\~ "'l,.\\.Si i.\\.1:1'-\\ I.OJ.'\\ -uf\\l.o "2.~.'\\1 -1.1. 1.\\i l't.'$.( C= Lnrvacwcighl (mgf= ll-A t.*'°"' t..ott te..S4\\ (o_'5'i. '$.11.:i L>.C\\G to.l.\\o *S.'lo~ <.o, \\"\\ s.10 S.lot s.'-!Cl Hnml c:ilculntctl. f\\n:ilyst: fi\\. ' Weight per inilinl numllcr oflnrvnc (mg)
= C / lnitinl 1111n1bcr.ol' lnn*nc 0.,
"'° 'lb' ~~ ~ "'" 0 '-bt
o
\\t~ I ~ ~* '\\..p~ ~ I c,, ~ '-::.:;\\ H:imlcnl~ \\,; \\(> t; ~ "°
~
c>> o* o* t:i* Annlyst: o* r:f o' r:l e>' .<<;)" b' o* Avcr:igc weight per , Pcrccmt reduction o.t,'-\\"1 -t-=- a 7. (). r;'\\8 -~.\\ 1. D, 51':-\\ - 0. q 7. . initi:11 *nunibe1* of
from <:onlrol (%)
_.Jarvac (mi?) Comment codes:* c*= clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm = unusually small, lg= unusually* large, d&r = decanted and returned, w = wounded. Comme1zts: I Page 29 of93 SOP AT20 - Exhibit AT20.3, revision I 1-01-14
1-j ~:~YF Page 4 of7 Species: Pimepltales promelas Client: TVA I Seguovah Nuclear Plant. Outfall 101 *. UV-trcated Date: 07-25-17 Survival am/Gl'owtfr Data Day 100% Intake Control - Non-treated Y Z ' AA_ BB CC DD EE FF .. GG: Jj_H
b 0
1 3 5 6 7 A= ran wcighf(mg) Tray color code:: '{}'\\~+/-o.... .\\n~lyst: 1¥'rYi Date: 07-2-4-(1 -B =Pan + Lafr.rv c weight (mg) Anulyst: --~'-------- Date:
qb*\\-"\\
Hand calcul:ttctl.A Amtlyst: ---i(;}.!,.i------ Weight per initial number orrarvn<: (mg) =CI lnilinl 1111111bcr orl:in*ac llnnd cnlcnlntcd. yl A1inlyst; ----~~----- .,\\\\*crngc weigh! per Percent reduction initial number of from control'(%) larv:1c (mg) 10 10
10.
IO 10 IO 10 10 10 I 0 \\ t> co l 0 /Q (0 10 10 J 0 10 co fe> I 0 lQ .10 t.0 IO fQ IQ f 0 IO tO. ID Io 'f 0 to to ca 10 tl) ro II,) LO 10 10 Jo to JQ IQ IQ 10 I 0 l 0 lO ['{) l Q
s. ~o s. '\\1. s.-s1 -s. S'S
'~~i0-~ ~ { - _ :: t..r..o r..ns. s.i.,,ti 1o.-:ib c-*-- o...,, Q \\_;. & 7. Comment codes: c =clear, d ""dead, fg = fungus, k =killed, rn = missing, sk =sick, sm =unusually small, lg= unusually large~ d&r = decanted and returned, w = wounded. Comments: J..J Page 30 of93 SOP AT20 - ExhibitAT20.3, revision 11-01-14
). Erw1ronmenialTestlng SolotJolis, Inc; c:c "<J~rih61o' llll ~!JtH~Ht' -1'1' A '.Con1rol *.
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TVA /Seq11oyah Nuclear Pfant,.Ou.tt'all lOl Jul11 ?5;;.August01, 2fU7 .:.,_~~ i~~u.:'-J /:-;-:::::;' Stntis1icnf. *A:n~lysc:s
Larval Flsh*Growtlt and Survl11:d*Test-7 Day Growth
/Start ~te: 712512017 End Date: ai112011 Sampfe"Dale<; July.. 2017 Comments~
UV-Treated*
Test ID: PpFRCR Sample ID: TV!'\\ I SON 1Q1 Lab ID: Eis:Enllir: Test.ing *~oi.
i~mP.re Type<.
0MR-01~cilarge Moliiionng Report Prol<Ji:ot FWCHR-EPA'.821 *R-62-013 Test.Species:
pp;PimC1phales promelas
cone-%*
1 ~ -- uv.con1ra1 -- *o.ssao
o.ssso Noo-<::onlrnl

o,6600

Q,6050 10;7 016120* '0,6480 21.4 0,6490*
0.5840 42:8. 0.6640 0.6090 ai;.s. o.57so 0.6060 100 0.61i0 0.5700 intake o.~oo o.5926 3 4 '0:5280 '1.6020 '0.5600. *0.6360 '0,5850 0.6030 0.6590 0.6400 0.5680 . 0;:3570
o:s5cio

o;s*r!lo
'0}~550
o:ssso 0:5400 0;5550.
. Transform: Unlr.insformod. 1-Tallcd .Isotonic cone-% Mean. N*Mean
Mean Min:
.Mar CV%.. .N t-Stat. *Critical.. llJlSD. .Maari N,Miian* uv.Gonlro!' iO!i685 0.9240 .0.5685. Q.52BU 0.6020* 5,533, .i*
,0;610.5 1,0QOO
(\\ICO*COnlrol Q,6153 t:OOIJO 0.6153 0.5600 o.s*sao. 7.017' ii 10,1.0.6238 1;0133 D.6236 0;5850 o.. 6500 ~.999 4. ,2,.501 24ttl' Q,0532 ti:6tQ5 t:aoca zr;~. *0.6030 0;$601. ti.6oso O;!i76ci o,549p* -5.42.1 ~-. -1,562
2'.410. i>;ass2* 0.6105,;oooo 42.8 ci..j465 1.0512. 0.6468 o;so9p 0.6641?
a,933* " 3;54~ 2.4.10 0.0532 06105 i:oooir .85,6 0.5978 0.9i'IG. 0.5976 0:5690 0:6400 61423 ~ ,1.:.124 2.'111) o:os~2 o..s!,r7a. \\),9791 100 o.$i3a **0,9325 0;5738 o.5460 o,imo
5;563 "
0.. 238
?;410 o.osn Oo/'.38 0,B39B lntai-:e 0.5585 o,9076 6.5535 05300 0.?920 4;564 4
Auxiliary Tasts .Stalislic; .Critical Skow. .Kurt* Shapiro-Wilk'slest i11tlieates normal dlstnbuUon (p> 0:01) Bartlett's TesHndica!es equal ilai'lani:eii IP-= 1.00) 0:94732. 0,.2Ji6' 1.71762 Q.684 .15.086J.

2A46El1 iO. t2992 -1.1710 lhe contn:il'ffi.,ans are'nol*siQniilcanllv dlfloront

100
ricatments vs: w;conlrol.. *--..

1).00353 '0.000913 '0:01933* o.05324 o;on:isa Linear lnlorpolatlon 120q RcsarnplDs) S5% CL:(EKP) Skow IC05 IC to IC15

IC20*

IC25 JC40 IC50 96.265 >106 >mo >100 >1D(I

,JOO

>100. Ul-.--------------. [!.9 lia* Oi? 0.6

~ (/.5 g:, O:*l
g,,1)3 ir: **0,,2 ll.1
0.11 Q>-<>--o:-~-__..r

-0;1

0,2 +..-....,..-..,-.-~~~-.--.-"'""..;.;..-i 0

so 100 0.5 ..... *...... ~.... *....... - .... -......................... - **** ~... ~ "",., *'I, 1.1a11, o.os revel 01 signlfieaiico *

~ OA

'3' - ~*a:J* 0.. a~ or 0 <i "' .0. =i Page 32 of93 .g .. ~ c:: e* z

~

~ ~ CJ

g:

..£ dt. s;1a ' Envlronmunt.i' T11s1lngSolul10n"..._f111;.:. TVA/ Sequoyah Nuclea1* Plant, Outfall 101 -.Intake July 25 - August 01, 2017 Statistical Analyses Larval Fish Growth and Sur.*ival_Test-70ay Growth Start Date: 7/25/20'1"/ 8/1/20'17 ,luly 2017 uv~Treated Test ID: PpFRCR Sample ID: TVA I SQN 101 - Intake End Date; Sample Date: Lab ID: ETS-Envir. Tesling Sol. Sample iype*. DMR*Discharge Monitoiing Report Protocol: FWCHR-EPA-821-R-02-013 Tes1 Species: l?P*Pih1ephales promeias Comments: Cone-% UV-Control Non-control 10,7

21.4 42.8 BS,6 100 llltak.e 1

2 0.5880 0.5560 o.6eoo 0.6120 0.6490 06640 0.5760 0.6050 0.6~60 0.5840 0.6090 0.6060 0.6170 0.5700 IJ.5300 0.5920, 3 o:s2ao* 0.5600 0.5850 0.6030 0.6590 0.6400 0.5680 0.5570

4.

0;6020 0.6360 0.6500 0.5760 .0.6550 .0.5690 0.5400 Q.5550 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max UV*Contfol 0,5685 0.9240 0.5685 0.5280 0.6020 Non-control 0,6153* 1.0000 0.6153 0.5600 0.6600 10.7 Q,6238 1.0138 0.6238 0.5850 0.6500 21.4 0,6Q30 0.9801 0.6030 0.5760 0.6490 42,a 0.646,8 1.0512 0.6468. 0.6090 0,6640 85,6 0:5978 0:9716 0.5978 b.5690 0.6400' 100

u,5738 0.9325 o.5738 0.5400 O.<lHO In lake 0.5585 0.9078 0.5565 0.5300 0.5920 Auxiliary Tests

'Shapiro-Wilk's Test indicates normal distribution (p > 0:01} .F-Test indicates equal variances (p ;= O:G8) The control means are not significantly different (p = 0.14). Hypothesis. Test (1-tail, 0.05) Hornoscedastic t Test indicates no significant differences Treatments vs UV-Control CV% 5:833 7.017 4.999 5.421 3.933 5.423 '5.563 4.564 1-Tililed N t-Stat Critical MSD 4 4 4 4 4 4 .i 4 0.473

1:94'3 0.0406 Statistic

. 0:93613 1.69266 '1.. 71762 MSDu MSDp 0.04064 0.07148 Critical 0.749 47.4683 2.44691 MSB' D.0002

Skew Kurt

-0:0833 -1.2168 MSE F-Prob df 0.00087 0.54944 ,. 6 Dose-Response Plot Page 33 of93 1-tail. 0,05 level (Jf significance l -I Species: Pimeplutles promelas Clieut: TV A/ Seqoovah Nuclear Pfaal. Outfall 101. UV-treated Dai{~* Clremistf]*: Page 5 of7 oatc: 0:t:.2s'" 11 D.a't' (Analvst identified for each *day, pcrforn1cd,11H~ D.O. and, conductivity measurements onlY.i* Concentration CONTROL UV-Created ll.4% 85;6% 100% l00% lutake 0 1 ~ Analyst* HS Parameter pH (S.U.) DO{mg!L) Conductivity (1111ihos/cm) kj\\lfrnlfnity*

c111g caco,11..J

~Hardness (mg CaCO;/L)

"'Temperature (°C) nH (S:U:) DO (1i1g!L). Coudu):tivHy* (1m1hos/cml i'*Tempcrature (0C) PH (S.U.) DO (mg{L) Conductivity (11mhos/cm)

Tcmperature'(?C) oH (S.lJ.)

D0(mglL) Conduclivity (iunhos/cm) . "Temperature ( 0C) CJH (S.ll.) D0fm!1/L) Condncdvity (µmhos/cm)

'~Temperature (°C) pff(S.U.)

DO (n1g/L) ConduclMty (fnnhos/cm) ~Allcnlinity .(mg CaC03'L) zwz. "'\\.c;. 0 "'\\..-<;. 0 210 IS.O . B-01 ff.o /~(_*--~~. :. \\~',,>>. LI~~ "Temperature <"Cl 1.'i. '2.. 1.Ll. b 1..4:.*1 {...'{.~ ~.Q -Initial Finni Initial. Finni lnitfal (..._:::-**- ;: -,,~ f;c : ::::, t*~t~~~ -, - ~ ~f!

!:_.._;:__..:,,:.-;_:;;J

'.to i;:lf,h*--- -~

_ !!~. -- ' - __ '~l ic.\\*.ln.

Final

. cmpcrnmrcs performed al Chi: tim;:: oflesl initiation, renewal or tcmiinntiqn by the analyst identified in the Daily Renewal lnfarmntion table loClltcct 'Oil Page I.

::.:J}JK* inity. hardness and total residual chlorine pcrforn\\cd by the nn~lyst ic:fcntificd 011 lhc bench sheet specific for each analysis and lnmscribcd lo this b~nch sheet ,.,,.*L*l\\y;,, _ Total residunl chloiim**vas pcrfonncd on.non-trcmcd OutfalHOl :111d lntnl;e samples. .SOPAT20-ExhibitAt20.3, revision I f-OI-14 l l J } J I

p~,.-?*

~r.:m 14 t~'-. Page 6 nf7 Species: Plmeplwleniromelas . Client: TVA /Se uovah NuClear Plant. Outfall 101. UV-treated Date: 07-25.;.17 Concen-tration CONTROL uv..:tre1.1ted 10.7% 2-J.4% 42,8% SS.§% JOO% 100% lnfake Analyst Pnr:imeter DO!m.;L}

Conductivity (1m1hos/cml "Alluilinity (mg CnCO;/L) '

'.>Hardness {mg CaC0;/L) Cmidi1c1M1y.

(~unhos/cm) *

"Allmliuity {mg CaCdliL) ,, ~ U:u*iJ ness '(m0.CnC0~). -*TR ehloi'inc.(mgll.),

Temperature (~C)

H {S.U;) DO (mg.IL) Conduc:livity hmihos/cm) "'AJl::llinity (n\\a CaCO,;JL)

Hardness

{mg CaC031L)

TR ~blorine (mg/L)

"'Temperature (~C) Dav

(Analysl identified fore:acll day, perfom1cd.pH~*D.b. and c.:inductivily n1ensutc111.:nts oril~*,)

3 4 5 6 Finni 1rimn1 Finai. Initial initial , * *** ~, ***.i... 1pcralurc~.p~rfomicd at 1hc rime of!esUriitiation, renewal.Qr 1cmiinalion by*ihc analystid,entined'in:lhe Dail}.,Rcnc\\\\'al Jnfom1aiion table located on Page; !, .. :";All*u 'nil)'., hardness am! tolal rc;;idual cJ1lorine*perfom1cd by U1c mialyst. idemificd on the*hcnch sheet specific for each analysis and:trnnscrlbed*tg tllis bench sheet ,'v 'Oy!"',, ~* T?lal 1rcsidt1ill clllorinc w;is perfonned 011 non-lri!atcd Outfall IO I and lmrikc sam)lles.

~ *Page 35 of 93

. SOP A T20 - E;xhiblt A 1203, revision l J-l} 1-14 I ' ] ' 1 Page 7 01'7 Species: Pii:uephales promeras Client: TVA /Sequoyah Nuclear Plant, Outfall HH, IJY-tt'l!ated Oat~~ 07-25-17 Concentratiorl Control Non-treated 'Analyst Parameter pH (S.LI.) DO (mg/L)

*Conductivity (11mhos/cm)

Alkalinity (mgCaC03/L)-
ffarclncss (mg CaC03fL)

"Tempcrntt1r:c ("C} Concen- 'tration Control Non-trcn tcd Parameter H(S,U,) DO (111_g/L) -Conductivity (11mhos/cm1

All;alinity (mg CaCO;/L)

"Hardness , (nig CaCO!/L) Analyst

Tern erature ("C}

Day (Anulvst idcritiffed,for_cncl\\ day, 11crfom1cd pH; D.O. and conductivitymensuremtnts only;) t 1 2 M.5_ -__ HS ,r-- - ~~"-~;,.** *1 v i ~ ;,..,........ "" --~.*~,..... J ' -._;_ - J-.,-., __ ""' .~ _,;: ( ' "~-.c:-" Initial [ Fim1l Initial Final Initial. I Final Day fAnalyst identilicd-for CDch.day, pcrfom1cd pH,.D.O. and conductiviLy measurements only.) 3 4 5 6

Initial Finni

'Tcmpernturescperformcd at the lime oftest initiario11, rencw;il or tcrrilinaifon by th" Bnalyst-idcntilicil in the' Daily Rc:n_ewal lnfommtion cabldocilted 01i:Pagc l. Alknlinil}', hardness-and total residual chlorine perfom1<;d by the analyst-id~ntificd on the bench ~hcct specific for each unulysls-and imnscrib~d to this l1enoh sl1ccl J hy:~~ Page 36 of93 SOP AT20- Exhibit AT20.3; revision Ll-01-14 (i11vifoo111en111t T<'Sllng Sotutfons, lnr.. nlNSUI DO (lnu/I,) {)lnll'HI,

Cu111l11cth-ilf (nmlm~/cm):

i'i1>it-11~*:1tt1)

\\lli111i11il)" (m1fll, CaC01) llur1lnc~s (mg/L CuCOil c::ontrol, tn*.11*c.:i1ed Tcmncr:thu*c (CL

. !Oii (Sill OO(mu/Ll Co11d11c1Mlv- (ittnhusicml ll11r1lncs~ (mf.l/L cncO,j lull !Slll 00(1111!/LI Comhsctivlt,* l1mi1in~lcml Teo\\11c1*ut11rc ("( J 1nll !SUI DO rnfr*/Ll C11mludii*ltv fumho~/cml till !ST.II no fmt!/L) Corul11c<lvit*; 1timhii.<lc-.u\\ lnll ISUI l>Olmri/Ll Co111luctlVi1v (umlmslc111) Temucraturc l"<'.'1 ull (.Sill OOlmg/l.I C1111d11ctMI\\; ( lilnho~fcn1) 1\\l1mli11i1y (111~/1,*cnCOJ}

'ru111l Rciililtrnl Chlorine ( 111!!/L Temnemturc /"Cl 11CJ (SUI DO lmt!IL)

ConduetMlv ( 11111hoslem I 1 o_u% lnlni<l' Allmliiiitl' (1111:/L C:iCO,) 'Tntill lk!lfll un I Chlo1*inc ( 111!!/L TVA I Sequo)'idt Nuclear Plant, O*rtfaJI IOI'? UV-h'.entcd July 25 M August01, 2011 Dovo Initial 7.86 v 308.

59.

S6 301 59: &3 24.~. 7,!l 194 24.9 7.% 7.!l 1&1 ~S.ll, 7.96 8.0 3.0 110 25.U 7.'16 s.o 193 71 73 .:o.JO. JS.I 7.99

SJ 191 7!

nl ..:0.10 /'hm:i1f111l.~.s prnmcllt~ Chrnnic Wb£Jlc Ernucut To~lc.lty*Tesl* EPA~21~1~-02-0J3, Method 1000:1) Daily Chemical Analyses llnvl Dav.2 !>:iv 3 .Initial F'iblll lnitii1I Finni

Initial llintil lniti11l Final" 7.88 7.90 7.73

$,I 1.1 7:8 JI I

N.5

.gs 7.71 7.7 2,1.6 7.83 i98 306 JA.2.

24.8 7.83 RGO 7.70 7.9 7,{1 193 7.88 8.,tll 1.70 7.Q 7.7 JUU 7:SS E.Ot 7.66 7.7 2.15 2*1,<.i 24.5 7.66 ~.O S.I 7:S 195 7.~3 s.o S.3 u 19.I. 7.77 7,7 313 61 .1~.6 7J!6. 1.S '307 59 87 7.86 7.87 7.8 7.9 257. 8.0

!U6 7,Sl 8.0 IStJ 77

<<O.IO

.<to 1R5.

71 7J ><CO.tu 25.U. 7.46 7;4!1 7 I 14.3 7,39 7.0 7,JG 7,43 <r.*~. 1,0 6,1\\

1.39 7 55.

7.6 7.0 317 2.:1.7 7.88 7.51) 7,l

JIO 7.87.

7.46 .-s.u 7.1 30S 2-1.. 8 7.~9 SJ! 7.0 300 7.SS 7.47. 261 s.o. 7.0 .216 15.U i.S~ s:n 6.8. 199 7;85 751 SJ 7.0 icl 7.0 .323 ' 5S S5 SJ! 31:\\ 58 83 &;O 3111 15,0 7,93 8.0 '.i.IJ3. 170 $.I

!!6

.S. l 1.90

.3.3 .:!03 77 <0.IO. 15.1 7.89 8.2 <0.10 15.2 24.5

1,63 7)-

7.59 7.2 24.5 7.1 6.ii 7.J9 tj.J '.!>I.Ii 7.J*I 6.0 24.u 7.45 -6.S 1'1'oj~c1111m1hrr:. ______ l_zs_t1_:1 0:1Y6. lnllinl Fimil . Initial

Fimil S.05 H10 7.51 7,IJ 7.3 7:3 7.l j}l 320

~4.5 7.9.t 7.59 8:03 7.50 7.6 i.O 7.8 7.2

Ill J!O.

.2'1:9. 7:95 7.55 S.03 7.47 J{I} J07. 14.8 7.97 7.51 7.6 6.9 7.9 7,4 188 296 211,9 7.97 1:5'1 7A3 1.6 7.1 8.0 6.6

!<IS 265 2<l..5 7.96 7.44 8.05 7.33 7.6 7.fl 8.0 5.7 214 2*L9 7.96 7.38 8;05 7.30 7.7 6, I Sci S.7 192 196 25.0 25.0 7.96 7.56 8.05 7.37 7:7 1.0
&.O.

25.0 25.0 1'1te: s.;inio1 _072:;i17<::1iem.xlsx En1.::red by; T SleeD.e' Reviewed py: -Jt'.- Page I of7 Ch1*onfo\\\\'hole EftluentToxicityTcsti(EPA-821~R-02~013lVfotbod1oo:tO) Speeies: Ceriodaplmia dubia Client! Tennessee Valley Autlioritv

Facility: Sequovah Nuclear Plant NP DES#: TN0026450 Project#:

\\-i.S& 'S Dilution preparaliou iliformatiou: Dihltion'pfcp (%) lfl.7 21..l 42'.s: Effluent volume (nil,) 2675 )35 (070 Diluent volume (mL) 22325 1965 1430 Total volume (ml..) 2500 '250(} 250(} Tesf.orrtallism source iu(orniatiom 1!5.6 IOO 2140 2500 360 0 '250(} 25Dv Or,wnism *age:

< '.N-hours old Date and times orga11isms were born
O"\\'"'"&ri Cll..~O ""to Ol\\\\.S

. between: Culture board:. m-t~*n A*

Replicate number; I I 2 I, I ~ I 5 I r. I 1.I..s_ I 9 I 10 Ci1ffure board cup number:

i.,,*111 t\\I l1l 1.'t 11.b I 1..'*H'?9. I ~2..1'3\\,; . Transfer vessel infomi:ition:. I PH = *:'\\'. ~k S.U. Temperature= t..'i.o"C. Average fl":IJ1sfcr volume (mL): I "'*0.25 n\\L

Daily re11ewiti iltfor.matioll:

County: Hamiltoi1 Outfall: 101 Comments: Test informatir.m: Randomizing template color:

£ncuba!OI'. t1Ulllbr.r 11nd sh~(f locntion:

. YWT aalClr: Selei.ms1r11111 batch: Dny Testfoitintion and feeding, MHSW

S:1mpfe numbers used renewal and. feeding; or b:itc11. u.~cd Outl1li io1 Jnmke terrnination time 0

07-25-17 07-26-17 2 07-27-17 3 07-28-17 4 07-29-17 Q'\\()'0 5 07-3'1-l 7 b8'1..6 7 .c:...-..::.t-i. . "'1...& \\ .a'l*~*r1 cn-o<o-t1 An:ilyst Comrol information: Sti1m1utrji oftest endpoints: Control-I Control.c.2 Acccptimcc cdtcrin % ofMaleAdults:

01.

01.

.s::ZO% 7-day'LCso )' /CX\\ 7. % Adults having 3'dBroods: I O<<l"i.. lOO 7. 2 60% surviving adults NOEC l (\\"? ~ % Mortality: 01~ c1~ dO'Yo LOEC ) (N'\\7. Mean.Offspring/Female' "'l..'i * '\\ l..'1*b 2 15.0 offspring/female

ChV

') f N'I. 7 %CV: '1.fi7,

q. () 1.

. <4i.O% . IC1s ') l'00-7. Page 38 of93 SOP A Tfl - Exhibit AT 11.2, revision H :o ! *-14 ~ r? ~--~-,:p1~ c ~ ~ I: \\:.:.:._: Page2 of7 Speeies;- Ceriodaplmin.tlubin Client: TVA rSeguovah Nuclear Plant; Outfall 101 Date~ 07~25-17 CONTROL-1 Sun*ival mu/ RepJ"oductiou Data Replicate number Day 1 2 3 4 5 '. 6 7 8 9 IO

)

Young t)roduccil ri 0 CJ 0 (\\ I 0 C\\ a r\\ Adult mortalily \\.._ '- \\._. ~ '- \\._ 2 Young produced (') 0 CJ 0 0 ,(") (\\ C\\ 0 a Aclnlt morlalif)'* L \\..- L- \\._ L-* '- L, L. \\._ ,J Young produced 0 r--.,, 0 0 a 0 0 (:'). l'"'I ~ Adult mort:ility L L. '--- \\._ '- 4 Voung produced '4. t...\\ ~- s \\..\\ ~ ~ ' v. s t..:.\\ Adult morl:ilit,1* \\.._ '-'. \\..,_ \\.._, \\._ L-s Young prodoccd* \\0 l"t) \\ \\ a,. 10.* ~- \\.,_ JO &'t) r 't.3 Adult morrnlilY \\.. L \\._ \\...- L \\,_. \\._., '- (i Young (Jn:iJ.lucctl D 0 0 ,('.) 0 G 0 0 0 n Adult 1nortality \\....:.. '- '- \\..._ \\._ \\_: '--* 7 Young pr_o_tluccd i"\\ L lg ~::, \\ l,g \\.~

l. £-

\\ '"-\\ \\ "'\\ \\~ \\ \\.::. To!nl young rroduccd t.& '?lo L."'l

,o

-z.'\\ ~L. !,\\o ~\\

,~

~o

Fini!l Adult Morlali~*

I*'.\\._ \\._. \\...... L. . \\._.. \\. .x for arJ 13roods. y ~- ~ )<. ~ ~ x "'.')(: Co11ceJilraJiow % Mortality:

07.

Mean. Offspring/Ferna!e: I -et."'\\ CONC: 10.7% Sun*ival "iul Reproductio1t Data Rcolicate 11umbcr Day j J 4 5 G I 7 8

n "

JO I:' Young pl"oduc:cd 0 0 a (') ('} 0 0 CJ 0 {-) Adult mor1ams I.._ \\__ L L L L \\_ L L... 2 Young produced () r, ' 0 6 0 6 0 n . () 0 Ailull mortnlity \\...... \\_ L \\._ L.. L L \\....__ L_ 3 \\'ouug producciJ ri () () 0 0 0 0 c 0 Adult mortnlily L L L L L L 'L \\,;._ 4 Young produced Y; 5 '-\\ s ~ ~ '-\\ ....:.\\ ~ \\.,,.\\ Adult mo_rt:ility L L L \\._ \\_. \\.,__ J 5 l'auttg produced

fl l1 IQ I

l 'O. .!"1.. }~ \\~ t.O 12 1*0 Adnll m11rtalit)* L \\._ \\..._ L \\....__ \\_ '- \\._ '- 6 Young produ~cd (") C\\' () ()

{>'\\

() 0 a I ('\\ 0 A.duJt*morlnlity \\....... L \\._ L. L L L 7 Young produccll l.~ \\ °' \\~ 1 l., l""\\ '\\ '\\ \\1,:. \\ C\\ \\ t \\~ 'folnl yoi1ilg product-tl !:it)

is.' o"Z.. b\\

~~ ~~ ~~ ~~ ~$ .~1- "FinnlAt!ulf Mortalily \\._... \\__, 'l._ L L.: I.._ \\. n*"o\\"ef (O sonn,c umt-11 oV.Ci v.-itf1 a(f.u1t rlunilc. lrnru crl., ' co,1ce11tratian: %Mortality:

01.

Mean Offspring/Female: .3?...8 %.Reductio_n from Control-I; -lc>o1*r?. SOP AT 11 - Exhibit AT 1 L2, n::vision ll-(ll ~ l 4 i ~ p; HS:>,:.:.!;~

~ :*? !'!.E.',

l ~.:~~NJl~J~~~~~ Pngc ~of:? ~I Species: Cerioilimlwfa ti11bia Client: TVA tSs;guovah Nuclear Plant. OutftilUOl

coNc: 21.4 %

Sm:vb10/ c111tl}1.epi*otlnctlo1i.!Yara

Renlic11tc* 11i1111bcr Day 1

l 3 4 5 6 7 s 9 {o 1 Young prodnccd CJ

o

.(") 0 t'l .0 0 b {')" ; [\\ Adult mortality \\...- \\..- \\..... \\_ \\,._ ,\\..., Young*produccd ('.. 0 D* ("\\ () '.6 a

o. a a

Atlu,f t.mortality \\ \\....;... '- L. \\.__. \\..._; \\..... L.. J \\'oung produced a 0 0 c 0 .o

(j a a

.f"'.'.. Atlul! mortality \\_..__ \\.._.* \\,,;..._, \\....._ '- \\.- 4 \\' oullg p1*0<1uc~d '4 '-:\\ q lo '-\\ ~ \\p. ',J\\ '-\\ ~. Adult'mortalily L L \\..,;.. ,\\,.... L. ' \\,,.... \\._ L L 5 \\'oung produced \\'D \\::S_ .t \\ ,.. c '\\L.. Yl-: 10. \\ (.;.' \\l \\1-1\\dult 1mirtality \\. \\.... ~ \\;_

c*, *\\.._'

\\....;.. '-- 6 ):oung produced D..o* 0 c o.* 0 ! 'O 0 - () .() Adult mortality L.. '- ~- \\- \\;_' '- \\,_ L 7 \\'ou_ng produced \\ l 1.Cl '\\ ~* \\\\o \\\\o. '. \\ '\\...\\ <\\ l& ""Lo \\\\, Tat.ii rmmg pradue:Cd !;)"'l..

~.~-*
~.

eol;) s~ ~s : :.s. '~':\\ ~.~ 3'-{ Final.Adi1tt Mortality \\.._ \\:._.... ~ '-- L.. \\.;.... O\\'"-" {olE.urin.\\?.cmir:d*ovcr with cduft durini:. han1fcr),

1Coru;e11lrf!ffo11:

% Mortality:

01.

Mean Off~p;ing/Feinnle: 2>9.ri; % Reduction from Control-I: ... '"'* b 7. CONC: 42.8% S11r:1c!ivc1( mu! Repi*o<li1ctkm Dalfl. Rcnllc111e numhw Day. I 2 3 4 5 Ci i 8 9 IO' I I \\'oung produced '0. {} (). () '(j () n 0 0 0 Adiil! JnQrtalily ~ L. 'L L

L L...

L:.. L-L I 2

Young:produccLI

.0

0.

("\\

G

()

o* (j' 0 () . Adult mortaliiy L \\_. L '--* L L L-L L ,,3 Yonng produccLI D I() D 0 a n 0 0 0 ' (} Adult mortalil)' \\,_ '-- \\;,._,.., L L L .L.L 4 Voung*prmluccd ' '-'{ \\o s .'S ~ "\\ 'I...\\ ~- ~ "'\\. Adult rnol"t:llit)" L L L \\.... L- .L l .*5 \\'oung produced l1,_ 10

1..

'.:!> ' \\,,,_ 1... l 1.. 0 l:b Adnltmorc:ill!y .L c L \\,_ L. i \\........ \\...... G Young,prodl}ccd rl Q, a u 0 ('i 0 0 0 .. 0. Ath11t mortality L \\... L L L L. L L '\\.,,..- L 7 . Yo_ung prod1iccd '"Lt:. '"'2.,\\ l\\ 'x "2..D \\. '( \\\\ '~ \\-\\ 1.6. Tot.al you_ug.producc(l ~\\,.

,.;l

-~~' !)\\co "&\\, ~'..\\. .i!J~ ~!..,. ~\\ .~q Final Adult ilfortalih' ,"L L L L. \\._ l__ c L

L L

aver (o 1snru1r: Qin,,. over wit " u l d~inu.l o:i.ns ~i. m h d I fi) Co11ce111mtio1i: % Mortality:

01 Mean Offspring/Fem::ile:

~s-.o j % Reduction from CC1ntrol-J: ..:1,.~1. Page 40 of93

SOP {\\.Tl l -ExnibitAfl l'.2, revision l 1'-0l-L4'

/ Page 4 0f7 Species: Cariotluplmin rl11blt1 Client: TVA I Sequoyah Nucle<ir Plant. Outfall 101 Date: 07-25-17 coNc: 85.6% S11n1t~*nl imilReprodudioi1 Du/11 Day I Young prodncctl Adult rnorC:ilir)' 1 Young produced : Adult"mortality J Youug produced Adult morta}ity ~ Young produced A!l!llt mortality 5 Young produ~c1l Adult mortality 6 Youl)g p1*od11ccd .Adult morfality* 7 Young produced Totnlyouug.produccd Finni Adult MorllllitJ* CONC; l00% Day J YOlll!g produced Adult mortnllly* 2 Young.produced Adult.mortality J . Yoimg produccll 1\\duit mortality .4 Voung. prod uccd. Auult morlalil)" .5 Yt:iung pt*oduccd

tdult m'Jrlalir,*

r.

\\'tiung produced Adult mortnlity '7 Young produced Tol:il young produced

Final Adult Mortality a '-

\\~ I () \\..._ Cl'. ' - (<) L l.. \\.._ \\.~ \\;_ r-1 "2,\\ I..\\ 0 \\.._,;.. 2 0 \\,_ \\~ 2 (') L-(':') L ~ \\_ '-4.. r-z.. a -ct. .,.,~ J Rcplit::ilc number 5 G 0 \\__ () \\_ 7 8 {"') 0 9 (')' L {'\\ 10 0 0 '\\_ 0 L 0 0.. \\....._..' . =:,. ' '"-\\ : s () \\..._ \\L_ D

C011 cemrm irm:

% Mortality: "L '-a \\.... .Mean Offspring/female:

%Reduction from Control-I~

S11n1hrn/*mul Reproduction Data

Rcvlh:alc number

'3. '5 G 7 s [) 0 0 0 (') 6 L ~ \\...... \\_ t'i t'i c4'i ("\\. 0 L* \\..._ \\_.L L L ('"\\ n (') () 0 0 L 'L L \\..._ L L <o L\\ s 'S s \\._ \\_ \\._ ~ '- t~ '~- \\L \\.!:) \\\\ \\ u. L. L L a 6 _() (\\ (\\ 0 ~ '-- L L L. '- \\~ \\ '\\ \\ *~ I..\\ -i.o ,_ 1.. ~-.;.\\ -~~ ?_,g ~~ ~"" ti\\ \\,_ L \\.... L LI. 0 '\\...__ L. [) L.\\

01.
1.\\.t\\ 1.

9 JO 0 c L. L 0 (') L L ('\\ 0 L L ~ ~ L \\"-\\ - \\..... L 0 (l L '\\.._. £.ei ai ~8. L ~. .'tote: Achih ~onalily fL n h... e. D,. d~'Old). sn ~split brood (s*nv.lc brgnd :p\\a bt;\\\\_\\'l;:'e:J IWO days). co:::. CllITV aver forfs:pnnc; ~rneJ over \\.\\ith ~dull dutiPt! tr.msfc:r). , Cm1cemfntio11:. % Mortality:. (\\ 1. Mean Oflspring/Fcmalc: ~1.~ % Reduction from ControJ.:t: - '21.b 7. Page 41of93 SOP ATl l -Exhibit ATI l.2, revision *ll-Ol-14 Page 5 of7 Species: Ceriodllplmia d11bia Client: TVA I Seguov:ili Nuclear Plant. OutfalHOJ Date: 07-25-17 CONTROL-2 Survflral mu/ Reproduction Daill Replicate number Day 1 2 3 4 5 6 7 s: 9 10 1 Young protluccd n (") 0 0 .0 -*a 0 0 {). f--.. Atllilt mortality \\... L \\... L \\.._ L_ \\'oung protluced f"\\ . {"\\ ('\\ (\\ (') (\\ ("\\ (\\ (') (') 1\\dt1lt ritOl'(:ility l L L L-L- L ~. '- l_* L .J \\'onng prmlucctl () ("') c-... ('"\\ () (\\ 0 c 0 Atl1ilt morr:ility L \\_ L L \\.....:. \\..-:

j Young*produccil

s

'-\\. "'\\ '-\\. s "?:, '--\\ c;

5.

'-\\ Adult morlnlity \\....: \\..._ \\.... .\\,.;._, \\..... \\.._ '- \\.._

i Young protlucc!l ll

~ "\\ ra to. 10 l '"\\._ 'Cl t \\ \\\\ Adult tnort:11ily \\._ *'-- \\..._* '- \\.._ '\\...... '- (i \\'oung protluccd 0 a a 0 0 ("\\ 0 0 ("\\ 0 Adult morrnlity \\_. \\.._. '- L 7. Young p;-ocJuccd l\\o \\~ l1 .f~ \\~ \\~ I\\.\\ 1"-\\ i~ \\~ Total young protlucctl

!:O ' ~Jfi>!. :,!;) "'
a t>

-Z..'\\ ~\\,) '1J\\ ");;~ ~\\ Finill Atlnlt i\\fortnlitr* \\_ \\_ L L ~ X for ~*d llroai:ls f'. ')c:: x.. X-

x.

Nm.!.:,,dulr rnonaluy (L""' lt,-c. D:::.. dcid), SO*- !=pin b1ood (smsl~ btood spin ~twern 1wod~ys1. CO""' c.:IT)' ov(f (ofT:;pnng cuni::d over w1th:idull dunns ~r:JrtSftrJ. Com:tti1trntiou: % Mortality: I Ql. . Mean Offapring/Fcmale: I "1.C\\.~ COiXC 100% Intake S11rvivq! and Reprodm.*tiiJ11 Data Renlicnte number Day I

?

3 4 5 7 s 9 10 I Young protlucctl 0 (J 0 0 () 0 0 () 0 rJ r\\tl111t murf:ilily \\_ L L. \\;_ L L L L \\..... L-Young pr-ad11ccd D

a.

{) 0 /) 0 rl. ("., (") ' ~ Adult m1JrlaliCy \\._.. \\.... L L L L. '-- L L . 3. Young 11rot1ucct1 0 0 ('\\ 0 0 () (") ('), () 0 Adult mo1*1111il)' \\._ '-- L L L L L L L

I

\\'oung 11rotlucctl ~ \\p \\..::. ~ ~ '-\\ \\,p \\.o ~- 1\\d11lt mortality ~ \\.._ \\..... . \\.._ \\....': L' \\..... 5 \\'oung protlucctl 1 :::.

~

"L' \\ 'S ' \\ I\\ 'l\\ '~ I\\ \\~ Adult mortnlity \\..;_I '-- \\_ \\._ \\._ L L L G \\'*rnng produced ri 0 0 0 6 0 0 0 0 Q. Adult morlality \\_ L L_ L L. L L L L. L 7 Young produced l.S;. l 4. li tS \\'\\. ' \\~ \\ ""\\. LS \\'5 11 Total young produce(! ~ ~& >5 '!1"1. '?:iS ?> \\ "1. '\\ ':>'-\\ ~l. :i'-\\ Fin~I Adult Mortality \\... L. L L "'- L. \\.._

over. {oiTnuiru! cariicd O\\'C'i wilh adUI~ iJurfr*.<t 1r.irur~r).

Conce11trntio11: % 1'1/ortali!y:

07.

Mean.Offspri11g/Fcmnlc: 33.~

% Reduction from Control-2:

-r~.~1. Page42 of93 SOP ATl l - Exhibit ATI 1.2, reviSion. I l-Ol-M "ti Q) ~ e 0.... co w " F~:l r--::1 *.. ~---* C t1vliQnmonu1IToulnJl Sah.111onr1 Int. Control-I OU\\' I

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.11 IO Ill JO 1~* 10 II 11 103 0 <l (1 0 n 0 0 () 13 15 16 14 14 IJ

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121 Q (l 0 0 I) u I (J 0 !j I\\> 15 l*I 1.5 15 17 161 Jl JS JI .:?'/ JJ 32* I J4 J.J.2 ..r..--- ~ -;--* . ~'if" @w. .. ' ~ t ! - ' -A-- . "ti._/ ;~- ~-~~1 r*--*- Ill CQ I CD t 0.... / co (o) Envlronmf?rital Testing Solutions, Inc. C'Qurrnlr:tlion ('Yu) Cnntfol - I !0:7% 21.-i'Y* .i2.s*1.. 85.6% 1011% Control-2 rno*v. lnrnl<c Outf:tll IOI: l)u1111cll's J\\'ISD l'nlur: l'MSll: l11111kr:* l>nn11~11*~ MSll \\':tlue: Pi\\J!:)ll, I 28 30 32 36 36 40 30 32 2 3 30 27 35 32 38 33 37 36 37 ,~5 38 38 28 30 38 35 LS.17 6.2 1.:521 5.2 TVA I Sequoyah Nuclear Plant, Outfall 101 July 25 - August 01, 2017 Chronic Whole Eflluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Ceriod11plmia du/Jill Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Vcl'ilic.atiou of Data Entry, Calculations, and Stutistic:1l Analyses Project n111i1ber:; 12585 l~cplicnlc number Sun*fr:ll

\\ \\*crngc *rcproductiim

(:r;erfitil'nl or ~ Percent r*ttlucliuu fJ om Hi (%) (offspring/fomnle) \\*:1ri:Uio11 t%J JJOntrtJ contruh: {"Ar,

I 5

6 I 8 9 30 29 32 30 JI

30 30 IOO 29.7

~.8 No11111plknhle 11 3<l )3 r ~ 33 35 ~2 IOI!

n.s 4.9

-JOA* JV 34 35 35 34 35 34 1110 34.11 6.2 -U.5 36 36 J.i 34 36 31 311 llJO 35.11 5.0 -17.S 38 38 37 35 36 ) JS IOll 36.2 5.2 -21.9 38 34 39 3/i 41 Ji 38 100 37_') 5.2 -27.6 27 30 29 .w 19

29 31 IOO 29.3 4.0 Nol apvli~al>l~

3l ~ 35 31 29 34 32 3*1 100 ~3.2 7.6 -13.3 MSD :. Minitnum Signilic'.ml Dillcrcncc PMSD= Pcrccnl i\\*linimum Sicnilic.*nl Diffcn:ncc J'f\\*ISD is a mcas1trc ~ftc~l pn.:ci~i11n. The l'MSIH; !hi: 111i1111\\lu1n pcrccnl <l11!l:rc11cl! lx:1\\l'Ccr1 the CJJntniland IJcatmcm that can bl! ucclnrcd ::talistic:ili~* $i;;nific:m1 in a \\\\'hole c(Jll1c1u 1ox1ci1y lc51 J.owcr PrvtSD bound <lclchnirml by US El' I\\ 110'~ pc1ccn1ik) = JJ'l"u. Urpcr l'/\\*ISD bound determined hy USE PA [90'" percentile)= ~7%. towc1 mill up1m l'JVlSD lioullds were dcicrmincd tro1111hc IOih uml 90th pcrcc111ilc, rcspe1.*1il'd\\'. ol'l'iVISD llaiu from EPi\\'s WE'f lntcrlnliornhil)' V;1nabili1y Study ClJSl!PA 20Ula~ USEP1\\. 20011'). I l~F.P/\\.. 21J(IJu *.'.!()()I b. flir111I Rcpon: ln1crl11h11rmf11)' Vmiu\\>ility S1rniy ol° lil'1\\ Sh<m-i.,1111 Chrome t>nd A.:u1~ Whole Ei'fl11cn1.T.. ~icily Test Methods, Vo!uml'S l :mJ 2-1\\pJ'o11di11. Hi'i\\-811-B~))-00,l oml IWi\\;S2J,HcOl-OlJS. lJ.S Eiwiron111cn111! l'm1cc111>n 1\\i;~ncy. Ciiicimmti, 01 I, ( -~..... i....,,.._ d\\ siarroaie;. 'l/2.512.01'1 TVAJSequoyah :N ucJcar. Plant, Outfoll 101 Jul)* 25,.. Au.gust 01, 2.0l 7 Statistical t\\nrilys~ . Cericd>if!hnia.SUrviVal :inc R<>produc=ticn Tcst-Roercllucticn TVAISQN 101 TmtlD; CdFR~P.- Sample JO;

eiid*Dale:-

.8/1/2017* Lab.ID: ETS;Enllfr. 'resting Sol, Sample Type< i;>MR,Dis_charge Monllorln\\l R~pcrt S!miple Dale: :July' 2017 Prolocnl:'FWCHR,EPA-821~R-02-013 Tes1:Specles: CO-CE!ifodajmnl;i*d!lbia CommenlS: Non-liealed Cone-% 1 2 .. 3** 4! .fl '6

7 Conln?l*1 2e.ooo 30,\\)00 27:000' 30:000* 291000 32.000' 30.000 Contt'ol*2.30.000. :28,000 30.000' 27..boo 30:000 29:000 30.000' 10!1'.

30.000 35,000 32,000* 3.}.000 34:000..3.3:000 33.ooo 21:4 :i2.oao..sa.lloo '33;000* 30,000 34.000 35:00.0* -ss:boo 42:8-' 36.acio.3r.ooo 36.000' 36.000 36,000 34.0p~ *34.000 85.if 3EfoOO

37;000. 35.000 fla.ooo.38,qoo 37.000 35000*

100 40.000*.:38.000 30.ooo 3.e.900 34.000 39,000 35000

lntflkl:.32.000

~s.~oo*.35.600 32.:000 ss:QaO 31:006 29090

.Tmnsfonn: Unlra*nsfOrmod

.C<>nc.,0/.

Moan..N-IVioan.M.,an

.Min.

Max CiOnltt'il~l 29>700* 1,0;*37 29~700. 27>000*

32,000 Ool)ticl-2.

29:300 1.0000 29.300, Gi'.ooo 31.000 10.7 32_.800 1.11.\\:ls 32.(:100* '30.006* :35,000 2.1.4 M.000 l.'11~04

.,.ooo.

30~000 :3s.oao 428 35.000 1.1.945 3!i.OOO* *3i.:OOO: 37;1)_00 as;6 36;200 1.2355 36.200 32:000* 3$.000 .100* 3?;060 1:29.35 .37.$00'

i1f:OOO 41:000 mta~e 33.200 1!13'31 33.20tl 2!);000

9$:000 AuxlllaryTcsts Kolmoaorov.O Test-Indicates non.riomiol dfstrib'!1ion* (p.,,. c;i.01)

B;!1f1.lett's Te.sl iniilcates equal va1ia11c;:e:s. {P = o.a111 The ci:>rittol means are not sicmlficanllv dlllerenl-ip = 0.50) Hypolht>sls Tost (1-tall; 0.05) NOEC.* *LOEC-- ChV.

S~l',._Marw-Ono*Rank Tesl*
100*

>:100 . Treatnienls vs Control~ 1

CV%

,tJ* '4,775" .ip, 3,957 10 .. 1,93-t 10

6,201 10 51040' 1P 5;175 10' s.106 iO 7.62"0 10 StntiStic 1:04609 1:SM68 o:sso52 a

31*.000' 29.000* '33~000 34;000 ~6:090

36:000

<11.. 000 34.000 Ranll Sum

146.00 150.00

.15'_3,50 15~..50 1s.s;oo. Linear Interpolation (2Q(l.Resol)jpll'.Js) .95%*CL .Skew Po*nt "la

SD-
9.

'30.00D ~.ODO

35.000' 35.000' 31>ililil 32;000

'37;000 .32.000 1*T'li1Dd Critical 75.'00 75.0ci 75.00. ts.oo 75.00 Critical 1.035-t~.0663 2.10092 10* 30.000 31,000'

32.000' 34.000*

34.000* 38;000' 3Bt000' 34c000

isotonic:

M~_:in

N-Mean,

34.267* *1-.0000 "34.261 1.0000 24.26/

1.0000

.94.267

'1.0DOO

.34267
1.0000

'34..267 LOOOO* Skow 'Kurt. -0.5496 o.~B5fl:4 ICOS'* ICIO' IC.W 1c20:* .IC25 IQ40' .u::.s:i*

>100

?-100 ?1110 "100

..100

.1'00

=-100 1:0,,...------------.

o.9 o.s; iJ;7 O,G o.~*

~. O,*(

'c. D:3 a:: 0.2

0.1.

0,0 * ;:. :.

O l'. '*
.0.2

~*?:1 * **.** _.._.;. -...... -D.3 .. Q,*I +-.--,~~.,.-.,.--~~..,.-.,.---i

o.

Ooso;R1>sj:lo_nso Plot* ~s....------------'-------------., '10 ~s 5 30:~,,._-"-i.--- ~ 25 "O ~-~9.

~ta*

10 s* 9:_+.. ---...; ... _---... -.--_ --...... _---.'!'!---..,---0.,------tl~, e

E

~ ~ o

_11:_

a E'. 6 6 .*Page 45 of 93 ~SQ Desso/a TVA I Sequoyah Nuclear Pfant, Outfall JO:! July 25 - August 01~2017 Statistical Analyses Used for PMSD calculation 0111\\'. Ceriodaphnia. Survilial a lid Reproduction Test*Repro*duction. Sia rt Date: 1/25/20~7 Tes! ID: CdFRCR Sarnpfe ID. TVA'{ SQN '101 End Date: 811/2017 Lab ID: ETScEnvir. Testing Sol. Sarnpfe.Type: DMR"Discharge Monitoring Repof\\ Sample Date: Jufy 2017 Protocol: FWCHR-EPA.821-R-02-013 Test Species: CD-CerTodaphriia du61a Comments: .Non-treated Cone-% 1 Controf-i 28.000 Conlrol-2 30.000 10.7 30.000 21.4 32.000 42:8 j6.000 85.6 36.000 100 40.000 lntak>? 32.000

2.

30.000 28.000 35.000 38.000 37 000 37.000 38.000 38,000 3 27.000 30.000 32:000 33.000 36.000 35'.000 36.000 35.000 4 5 6 30,(100 29.000' 32:.000 27.000

30.000 29:000 3:1.000 3<1.000 33,000

.30.000 34,000 35,000

3G.OOO 35,000 34,000 38.000

'38,000 37000 -38.000 34.000 39.000 32.000 35,000 31,00Q Transform:. Untransformed cone-% Mean N-Mean Mean Min

Max Control-1 29.700 1.0137 29.700 27.000 32..000 Controi"2 29.300

1.0000 29.300 27.000 31.000 10,7 32.800 1 '1'1$5 32.800 30.000 35.000 21,4 34.000 1.'1604 34.000 30.000 38.000 42.8 35.000 1.1945
.5,000 3'1.000 37.000 85.6 36.200 1.2355 36,200 32.000

38.000 iOO 37 UDO 1.2935 37.. 900 34.000 41.000 lntal<e 33,200 1.1331 33.200 29.000

'38.000 Auxiliary Tests Kolrnogorov D Test indicateS non-normal distribution (P-= 0.01) Bartlett's Test indicates equal variances (p = Q.89) T11e control means are not s!Qnlficantly different (p = o_5.0) CV% 4.775 3.957 4.937 13.:201 5.0<10 5.176 ~.19('; 7.620 Hypothesis Test ("I-tail, 0.05) NOEC LOEC Cli.V . I u. ounneu*s Test 100

>100 1

. Treatments vs cont<Ol-l 7 30.000 30.000 3~>'.000 35.000 34.000 35.000 36.000 2~toOQ. N 10 10 10 10 10 10 11 (I 10 Statistic '1.04609 1.68468 0:69052 s 3.1,000 29,000 33.000 34".000 36.000 36.000 41,000 34.000 t-Stat -3.837 -5.323 -6.561 'B.Otl6 ,10.:151 .MSDU* MSDp 1:84724 0.0622 9 10 30.000 3.0.000 29.000 3'!,000 35,000 32.QOO 35,000 34:000 31 090 3.\\.000 32 000 3f..000 37 000 38.000 32.000 34.000 1-Talled Critical MSO 2.287 1.847 2.287 ',8,17 2.287 1 ;Bt17 2.287 1.1347 2.237 1.8'17 Critical Skew* Kurt. 1.035 . *0.5498 0.48564 1.5.0863 2.10092 Mss* MSE F-Prob df 81.1067 3.26296 6.6E-13

5. 54

\\ TVA /Sequoyah Nuclear Phmt, Outfall lOt ~Intake July 25 - August 01. 2017 . *-. ~; Sta1islical Atialyses Environmental 10?Sllng Solutions *. Inc.. Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 7/2512017 1est ID:* Cd FR CR Sample ID.: !VA I SQN 10~ - Intake End.Date_: S/112017 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Dlscharge. Monitoring Report Sample Date: July 2017 Protocol: FWCHR-EPA-S214~-02-ff13 Test Species; CD'Ceriodaphnia dubia Comments: Non-lreated Cone-% 1 2* 3 4 5 6

7.

Control-1 28.000 30.000 27.000 30:000 *29_.000 32.000 30:000 Control-2 30.000 28'.000 30.000 27.000 30.000 2!thl)OO *30,000 10.7 30.000 35.000 32.000 31 000 34.000 33.1)00 33.000 21.4 32.000 38.000 33.000 30.000 31\\.000 35.000 35.000

42.8 36.000 37.00_0 "36.000 313.000 36.000 34,000 34.000 85.6' 36.000 37.000 35.(100 38.000 38.000 "37.0.00 35.000 JOO 40.000 38.000

'38.000 38.000 34,000 39.000 36.000 Intake 32.000 3~!".000 35.000 32.000 35.000 -31.00D 29.000 Transform: Untransformed Cone-% Mean N-Moan Mean Min Max CV% N Contro1-*1 29.700 1,0137 29..700 27.000 32.000 4.775 10

control-2 29.300 1,0000 29.300 27.000 3.1.000 3.957.

10 -10_7 32.800

1 1195 32.BOO 30.000 35.000 4.937 10 21.4 34,000 1 1604 34:000 30.000 38;000 6.2Q1 10 42.6 35,000 1 1945 35.000 31.000

.37.000 5.040 10 85.6 3$,21)0 "1.2355 36.200 32,000 38.000 5.176 10 100 37,~100 1.2935 37.900 34.QQO -11,000 5.196 10 1ntal<e 33.200 1:1331 33.200 29.000 38.00"0 7.620 10 Auxllia!_1 Tests Statistic Shaplro-Wilk's Test indicates normaldlstributie>n (p > 0.01) 0:95353 F-Test indicates equal variances (p = 0.03) 4.76033 lihe control.means are no! significantly* different(~"" 0.50} 0.69052 H~J!Olhesis Test ~1-tail, 0.05) .MS Du Dunnett's Test indicates no significant differences 1.52244 T:realments.vs.Conlrol-2 Dose-Response Plot - Page 47 of 93 8 9 10 3:LOOO 30.000 30.000 29.000 29,000 31 000 33.000 35.000 32.000 34.000 35,000 34.000 36.000 3*1,000 34,000 36.000 32.000 38-000 41.000 37;000 38 000 34.000 32.000 34.000 1-Tailed t-Stat Critical MSD -4.432 1.730 1.. 522 Critical 0.868 6.54109 2.10092 MS Op MSB 0:05196 76.05 1-laif, 0.05 !evel of significance MSE 3.81222 Skew 0.20974 - F-Prob 3.2E-O<l Kurt 1.47265 df

1. 16

J ~~1::~hlttn~1~~~1~~- Species: Ceriotlaplmiti duhirr Client: TVA I Sequoyah Nuclear Plant, Outfall 101 Daily Cl1emistrv: Ony Pf1ge G of/ Date: 07-25-1'7 (Analyst identified for each dny, pcrfom1cd pH, D.O.-ru1d conductivity mcasuicml!lils univ.) Concentration CONTROL l0.7% it A% 42.8% 85.6% l00% 100% Int:ikc Analyst HJ Parameter pH (S.U.) 7-flfi, Co11ductivit}' (µmhosfcrn)

Al!mlinitv (ing CaCO~/L)

~Hardness (mgCaCOjJLJ "T<impcrature (0C) ui-I(S.U.) DO(m#L) Conducti\\'ily (µmhos/cml '"'Temperature (0C) nH (S.U.) -DO (mg/L) Conductivi1y (Jmth6s/cm) -i<Tempernture (°C) pH(S.U.) DO(m.!1/L) . Conductivity * (11mhos/cm)

Temperature (0C) 111-l (S.!J.)

DO (ml!/L} Conduccivirr (iunhosfcm)

Tcmpcrnturc (0C)

'nH(S:U.J DO (mg.IL) Conductivity (µmhos/cm) "'Al kaliuily (mg CaC03'tL) ~Hardness (mg CaCO;/L) "TR chlorine (mg/L)

Tcmperatura c*c) uH (S.U.)

DO (mgll) Conlluclivity (µmhos/cm) *

Amnlinity (m~ CaCO;/L)

Jlnrdncss (mg CnCO,/L).

"TR chlori111: (mg/L)

Tcrnpcr:iturc (°C) sos B-'

"l5,.\\ Initiiir 0 I Z rlS .HS - _,_,,. - -*.~""'.:' LS.l ,c;. \\ "1.S. 0 Vinal lniti:il Vinal lnitinf Finar

1 c111pcratures p~rformcd al the time of lest initilltion, rcnCWill or termination by lhe.nnalyst identilicd* in the Daily Renewal

~")' lnfonnatiun table located on Page I. Alkalinity,'hardness and total rcs_idual cjlorinc performed b}' th<! analys1.idcnlilicd on the bench ,.,,~::::~~-"' . ~[1ecl *peclfic forc:1c11 mrnlysis and transcribed to tilfs bench sheet by: ~~--* ~ Page 48 of 93 SOP ATI I -ExhibitATI 1.2, revision 11-01-14 l Concen-tration CONTROi;. 10.7% 21.4% 4:2'.8% SS.6% 100% JOO% lntnkc J ~-~:::~,r~1~w.. 1;::,fl~ Page 7 of7 Species: Ceriot1110J111it11/ubia Client: TVA I SeauovahNuclear Plant. Outfall 101 Date: 07-25-17 Day (Analyst idcnlificd for each duy, pcrfom1ed pH, D.O. and conducliYily measurements only.) Parameter pH(S.U.) DO Cml!IL) Co1fductivily (uinhos/cm) "Allmlinit)" (mi? CaC03/L) ~nnrdncss (n1g CaC03/L) Analvst

Temperature C°Cl 00.(m!!IL)

Co11!111c1i'Vity

.(1m1hoslcm)

Temperature (0C) nH (S.U.}

DO(mwLl Conductivily (µmhos/cm)

Temperature {°C) pH (S~U.)

DO{m!i/L) Coml11c1ivity Hm11iosicm) "Tcmpernture c<'CJ riH (S.U.) DO (mg/L) Coµdi1eth*ity *

{µmhos/cm)

'°'Temncrature (°C) DO(mg/L) ConducliYity f1m1hos/cm) "Alk.11inity (mg CaCOi/L) ~Hnrdness (mg CaCO;/L} ~TRchlorlnc'(111g/L) * * . *Temperature (°C). pH (S.LL) .DO(mg/Ll ' conductivity (µmhos/cm)

Alk:tlinil);

(Ing CaCO;/L)

Hnrdncss

. (nil? CaCO;IL} "TR chlorine (mg/L)

Temperature (°C>

3 4 5 r '~.~~l~j ( r

~ -~~~::-"d L-*.:~.: ""'-! 1.1'- _.
-:' "l 6

!_f --v::-,_~-.. ~:i ,\\ f."-'-", __ :_.,..!__:.::.. t ")..S.\\

,;,

,....,~..,,.,,

-1,_*~! ~"~ -:-~~: :11 lniti:il Finni Initial Finni Jniti:ll Final hiitial Final "Temreraturcs performed at the time of ~t iilililltioi1, renewal or 1cnnina1ion by the analyst identified in the Oaily*Rcncwal lnformatii;m tab(e:locatcd on Page I. Alkalinity.,lmr'dn~ss and Iota! residual ch orinc pcrfom1cd by'lhc ~nalyst identified on the j:lcnch .sheet specific for each analysis and transcribe~ to I his bench sh~cl by: _

Page 49 of 93 SOP AT I I - Exhibit ATI 1.2, revh~ion I i'-01-14

Envlr.;imn~ntal Te~lln!) Soluclon*. Inc. Gunccn11111lo11 Puramercr 11lr(Sl!l UO (rnl!ILl ('.0111h1ctil*it1* (11111ht1~lr111) ('tm1rul Mlwli11io* (111g11. c.,co1) I lard 111!!.S (iug!L Cn CO 1) Tc11111c1*11t11 l'c (C) lull /Slll UO!mwl.) 10.7% Coaul11e!i\\oi1v (11111hoslc1111 Te11111cmrnrc ("C) nil (Siil 1)0/m!!/Ll

ll..J%1 C1111duc1i1*i11* (11111hostcm\\

'J'cnme.nuurc !"Cl un !Sl!l OOhlil!ll.i 4Z;R*Y. Conlluctl\\*it'* (mnlms/i:mi Tem1icrn1urc f'Cl !nil (SU) IJO!m~IL) 85.671i Conducfi\\*it1* f umhoS/cm) Tcmpcro11urc ("CJ lull (SUl OOJ1t1!!/Ll Cooduc1h*i1v hnnho*frm1 1(11)% Allrnliully (111!(/L CnC01) ll:ardnl"Ss (ml!}L C:1CO,) Tu!nl f{csitluiil Ghlorlnc (m~/L) Tcm11c1~1111rc tC) oil ISIJl l>Olm!!ll,) Co111luctivi1'* (1111'ilros/c111\\ lllll'l';, lu1~hr i\\llmlioity (na!(l'L C11C01l lhmlnns (m!!/L C:iCO,) To1;1l lh'shlu:1I Chlorine I 1111.:/Ll Tenniei*:itu1*c !"Cl TVA I Sequoyah Nucleal' Pinnt, Outfall 101, N1)n-t1*ellted .July 25 - August Ul., 20li nn,, u lnili:il 7,R6 7.7 308

59.

86 1"-1 7.90 7.7 292 1~.8 7_91 7_3 280 14.8 7.92 1;lJ .254

N.S 7-92 7.9 207 14.S 7.. 90 s.o 194 71 73

.;:0.10 24.9 7.91 SJ 194 69 77 <O. lO

?5.t Cerimlaplmin t111hht Chl'(lflk* Wholt Efllul~ut Toxicil)' Test F.PA-821-R-02-01.3, Me-U1od 100~.o Onily Ch(~micnl Arrnlyscs D:1,* J D;11*2 D:n* 3 l'iu:tl lnilinl Finni Initial Firm I
lni1inl Fi1f:1I 7.95

- 7:90 7.K3 7.77 7.Sli 7.89 7_lJ] 7;(J 7.7 7.6 7.7 7.S 7.6 ~-0 311 313 317 61 87 152

!~.6 25:*)

14.8 2~.8 24.S 251) 7.95. 7.95 "82 7.84 7.80 7.90 7.91 7.7 7.b 7.G 7.7 7.7 7.6 g.o 291) JOI 305 14.S. 1*1.7 J4.S 1'1.3 14.7 2U 24.8 7.95 7.% 7,82 7.84 7,79 7.90 7110 7.7 7.6 7.0 7)l 7.i. 7.6 S.u 294 .19:? 304 24.S

24_7 250 2*tS 24.S 2.U 2*1.S 7.95 7.91 7.&4.

1.85 7.78 7.'JO 7,S9 7.$ 1:7 i.6 1:3 1.7 1.i> '3,1) 169 164 171 24.9 24.7 24.9 14.9 2~.3 14.X

!-L~

7.95 7.96 7:84 7.85 7.77 7.88 7,89 79 .i.7 7.6 7.S 7 <! .tl -:7 3.0 211 207 2U} 25,0 20 24.!:1 14.9 24.S 1J.3 24.9 7.95. 7.97. 7,85 7.S6 7.76 7.Sf* 7.SS. 8.0 7.9 1.B 7.8 7.S 7:7 S,' 1.94 190 199 69 77 <I). HJ. 24.8 2-l.7 25..1 25.ll

!4.9 14.9 15.0 7.. %

3.00 7.85 7.86 7.79 7.!!8 7.92 S.0 7.9 7.S 7.fl 7.9 7.7 _::U 193 187 196 '7'1 73 <0.10 25-t .'.!4.[, 25.l 25.0 24.'i 24.3 2~.9 D:w4 111i1i.:1I 8..D2. 7.J 323 5~ SS 2;.i.!i 3.01 77 JI 7 .N.9 7.98 7.7 JOI 25.IJ - 7.98. 1..1 271 25.0 7.97 7.6 217 ~5.0 7.96 7.3 200 1'>9 77 <0.10 '.!5.1 7.96 7.3 19~ 69 S\\I .;:0.10 _.!jJ) .Finni 7.92 7,6 24:9 7.91 7.6 25,l 'i.91 7,6 25.0 7;SIJ 7.6 - 25.0 7.SS 7,6 ]5.1 7.88 n* ~4.9 .7.95 7.7 15.0. l'r*ujcirl 1in111h1"~ ______ 1_2_s_s:._* IJ;11* 5 Day 6 lnilial S.05 7.6 J24. 24.7 3,02 7.6 Jill 24.8 '8.0T 7.'11 191. 24.S 8.00. I.ti 264 2-l,$ 8.00 7,(* 21.1 24.9 7.98 7.6 i.n . ~-..... 15.0 7.98 7.5 1as 25.I Fi11;1I luilial* Final 7.99 g;11 7.91 7.S 7.8 .to 320 25.0 24.6 25.0 7.97

i:w 7,89 7,1; 7.6 s.o J09 24,8 24.6 1.5.2 7.97 S.!U 7.86 7.S 7~6 8.0 301 24.7 24.ti 15.Z 7:95

. s:11 7.26 'J.S 7.7 l:l D 272 24.7 24.6 25.1 7.93 KIO 7.S2 7..S 7.7 SJl 217 24.9 24] _2.:!.9 7.91 8,08 7.S*I 7;9 7.. 7 7 *) 201 24.9 24.9

~5.0 8.01 8.09 7JJ?

7.8 7.7 7.~* 193 14_!1 24.8 1*1.il Ffle: sqn-l 01_072517cl1em.xlsl1 Entered by: T.S'f).per Reviewed by. \\

' Enlrlronhl*nt*lle:nlog,S<>lllt16n>iln<<

Toiaf.Residual Chioduc(ORION-l9"77) Matrix; Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Page &~ Page _/__of _J__ Analyst I L7J"'.

Dat~*analyzed. c)"':J *ZS:--/"J lodi<lc reagent: I IJJ/? 7 "7 "I Acid reagent:. I f'Jl?..8-=t8t!ff-Calibration:

~J crf-ZJ?=:l l}.10 mg/L J;OO mg/L mV Change (suggested rlinJ?e = 26 to JO mV) Reference standard munbcr J )J.C.S /6.;1-S I J.:J.J'.J 1'7=!fJ Millivolts (mV) s-to. "I £t"f"1. ~- -Zt,.. 9 Nore: f'or samples with a residual chlbrmc 01 > l.O mg/L.. the ~:imp!!:!; must. hc.ililutcd 10 be. w1th111 the i:nl1brauon range. Lnl>orotvr)* control stamlllrd: Reference standard Truevalu.e (TV) Measured value.(MV) % RS= MV /TI1 x.100 number (mg/L) (mg!L) (ncccptabh! range =.90 to 1io%) 1,JJ.J Jl11f!. 0.50 b-53"1 I tr:G-

4 '7,..

Sample cha1*actcristics Residual chlorine (mofL) 'YaRPD = f(S - D) /[(S+D)/2Jl x JOO (acceptable range"'+/- 10%) I S,t, 0

DOJ..r l,

Duplicate l.o. ol!::!3 s i amo, e.measuremrmts: Sample Snmplc ID Siimplc cluiractedslics Residual chlorine .. number {mglL) Reai:?.enl Blank ~ -~- ~~-- - Lo.ool, t'101t..."-\\..0 \\, '""' \\. SQ.t-J \\C'.i' J)./J 1~~ ~--1 rid,,,,. 0-oriZ r1lh'1.L\\*ISL- ~* l i-JT1'\\<E: .Aro C~c [;) Q.f!Vi "" '.......,,.., *..., i l / /'I / J'-f _jl ./ I l \\ -~ J - / u=r-z:J, t /' v Note:.'\\II sninplc:; were annlyzed mexcess of EPA recommended holdnig 11m~ (15.mmutes) unless otherwise not~d. Labora((Jr

c:outro.I standtml:

Reference standard number. .. f\\)~ V/,.* B Page 51of93 True value (TV) (mg/L} 0.50 l\\1casurc.d \\'alue (M\\I) (mg/L)

o. V:

3 % RS= i\\'lV /TV x 10() (nrccptablc range= 90 to 110%) Reviewed by I ;t( Date reviewed ~==~,;...~rl-l..:.'-_--1.S;~-_,_--1_,J-L-_-_-__,-1 SOP CS-Exhipit CS. I, revision OJ-Ol-*13 ,_ ;*-:=.:J ~, r:,i ~= :: r.. _. \\ Environmtmral Tentn~ ~olulians. ltica Dale analyzed~. U ~1-:z Analyst I rf""' Ctilibratiou: Rcforence:standnrd numbl!r Millivolts (tnV).. TotaJRcsidunJ*ChJOJ*ine (ORION-1977) Matrix: Water, RL = OJ o mg/L Meter: Accumet Model AR25 pH/Ion l\\foter. !),10 mg/L 1;00 mg/L Page l,3 l:'age __ /_of-'-- mV Cliangc (suggested range"' 26 lo 30 m \\I)... I IVS~ 1'11-e:, IAJ.S.J /o/yf!, 5"'1-->,. I 5'7'H. 9 L_-=l,. 8 Note: for samples with* a rcs1tlual chlorine of > LO mg/L the-samph:is must be*dilut~d l(* ll"* within !he cnlihratio11 range. LaboraliJI]' co1tlrolstmrdard: Reference stnndard True value (TV) Me:isurcd*va!uc (!\\'IV) % RS= MV /.TY x mo number (mg/L) (111g/l.) (ucccptntllc range= 90 fo ll0%) }JV.ff J'7-!'d O;SO

o. c::>J <f J.os~ ff 7=

Dupllcate sam11/e prec/slau: SamplC Sample ID Sarnplc characteristics Residual chlorine %'RPD = {(S - D) l!(S+D)f2]} x 100 munbcr fmg/L) (ncccpt:Jlilc nmge =+/- 10%) \\\\lP\\\\SG..N \\O \\ s r'la'\\"z.b.. o \\ ?alt *-'AJn:i _..,&nM' L~. oo(o. l Duplicate D lo.oo_"'I S I amp, e measurements; Saili(llc SnmpleTD Sample characteristics Re~idunl'ehlorine number (m!!/LJ Reagent Blank ) o-oos \\ 1 tr\\ c..r.!). G'l.- T\\J~ \\ 'S.G_~\\Q\\- \\ "t.l'\\"l'l\\,.\\<:...12. PalP ~ ij--~ ~ O.~DZ 7 / / / f-1 l / I .A~/.... Llf / UT/Joi v / / ~- Noie: AWsamplcs were analyzed iii ei-;cess ofEP/\\ reGomml!mied !\\Olding time-(15 minu1e;) unless olhcrwise*notcd, L l t I I '/ I

a Jormorv con ro,-; a1u 11n :

Rcfct*encc stnndard True 1*alue (TVJ number .(mg/l.) }f\\ ~~ J1r=IP. {J.50

Page 52 of 93 Measured \\'<lluc (MV; (mglL)
o. 5'tt.

% RS= MV /,T'\\l x 100 (ncccptalilc rnuge = 90 to 110%) ldt- ?!?- Rcvic~cJ by j1--::c\\!~,.,.---~J Date reviewed '--_0'1_"..;;.'Zli_*... IJ_.__--'J SOP C:S - Ex11ibit CS. I, revision 01-01-13 ~*. I ~ r~~ 1 1 -1 <.:., t:c:: \\ .\\--------

;) Envlronmen.t*ITeiling Solulion1. lnc.

Analyst r "\\k} Date analyzed : d) *Q.61)* Calihmtiou: Reference standnrd numbe.:r Mi!livolts.(mV) Page lo'-/ Page_L.of_I-Ttita.1 Residual Chlorine (0Rl0N-l977) Malrix; Water,:RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter O:IO mg/L Loo ing/L 1 tJ~s \\1.nP> \\ *N.:> S 141-e "1l 2 ?_ (, "" 1. (o todide reagent: I \\ J\\J.£... -:::iq'1' Acid reagent: _ I M/t. &ft m\\I Change (5ugitcstcd rnnge*= 26 to.JO mV) ~°'* t.J Note; For samples with a residual chlorine of > LO*mg/L, the samples ruust be diluted lo" be withi11 lhc calibration rnnge. Laborator comrol stamfllrd: Reference s!nnd:ird Sample number .number redsio11.~ .J Duplicate S I amp, e mens11reme.11ts: 1'rue value (TV) (mg/LJ 0.50 Sample chnrncieris!ics (t~. (J.t;()I Sample Sample JD number Residual chlo1'ine (mg/L) s LD.. D D % RS = J\\'IV I TV x J 00 (:1cccptnblc range =*90 10 110%) %RPD "= {(S - D) /[(S+D)J2]) x 100 l;m:c11tablC range=+/- 10°/o) $ams)lc ch:u*:'lcte1*lstics Residual chlorine (mg/L) ~- -- - -- ~* R~eent Blank .l.o. o~s 11-01Z8DZ... ~At.\\ ~Gt~ 10\\ -. 1~""2. Do J P. 1-tt.n. LJp(P .(Q. () i.8 I ~ .\\.\\1/~ ~ ~ ~ ~ Nore: ATT samples were analyzed Jn excess of EPA.rccommcnded*holdrng, time (I ::i mmulc~1 unless otherwise noted, Laborotor 1 c01itrol s/imtfard.:. Page 53 of 93

True \\'nluc (TV}

(mg/L). 0;~0 Mcasured.,,alue (MVl (mgll~) O.oD % RS'= !\\'IV /TV x: 100 . (11cceptabr~ range= 90 tolf{t%J . Reviewed by lf---...,,V.:P-_* -----i Date revkwed.

rt

u ~n SOP CS - Exhibit CS. I. revision 0 I -0 I-! 3

1-1 0 r-> *ic*,'_:j,.~ a 1

1.

....:.~ .t - ~ ~*-.--' ) Envlronmwtal,le.sting S'31tttlont. Int.. Analyst I ;vL Date analyzed* 0 ~-2 J: r1-Alkalinity (SM 2320 B..;i.997) Matrix: Water, RL=.l.O mg CaCO;/L Tifrate samples to

pH=4.S S.U.

Page 66 Puge_-"'-_of "-\\ "Time 1.*nitiated' *~-rn~B?...__?,,,.. ___ __, 11me completed. oc,~:::j '--"~-=--L.------1 Tr I l'/' l 11ra11t uorma 1tv am mu li/J. rer' etermr11alt01': pH of i'iormhlity . Nornmlity (N) of n 1so.1 pi£ F;ictor or Multiplier Deionized Tit rant' check Begin End Total = (5 m'l Na2C03 x 0.05)/E. = (N x 5000.0)/ 10() ml sample water refercnca* st:rnd11rd ml' ml ml =0.25/E =NxSOO =4.SS;U, number number CE) ...(acc:cptablc range= 0.0180-0.0:220) 6.0 \\h)t1ttM. \\ J.\\SSr~-8'-\\ D* \\ .\\'l:S (1-~ o.o*zo z..

10. \\

l!?I\\ o.u-O.\\ ;..0~1rn1 Luborator~sc;t',dard: Rcfe1*cncc standard True. value Somp!e. / Alkalinity (MV) * % RS =' (\\'IV I TV ~- JOO nuniber (TV) volume Begi!I End Tomi: Multiplier. (mgC:iCO.v'L) (ac~cptablc range (mg C:\\C03/LJ* (ml) ml ml .ml = 90 to. llO?/o) \\~'SS\\~~o 100 100 12.5 "~*3. Cl.~ lo~l q°' qC\\.Ol. D l 1111/icale samo. e 11recisi011:* Sample. AH<:ilinfty %RJ.>D= Sample Sample lD volume Begin End. Total Multiplier (mg CaCOJIL) [{S'* D} /l(S+D)/2)} ~* 100 number (inf) ml ml. ml_ (acccplnblc rilnge=+/- 10%) U).t-e.n* 6slAJ 100 ~i3 l"sA, -3.f 1*0, \\ s 31 ~ ...\\ Duplicntc (B) _j ~S.Y.~es "5.' ..l D

31

-t'-01-Utl-Hi "{j 1 ' alnx. Sill ce reco11ery: Reference standard Spike y;1luc Sample Spil<l.\\ alklilinity (A) number (SV) volume Begin. End Total Multiplier (mg CaCOJ/L) . (ml! CnCOJIL) (ml) ml ml ml I N.5srSS"o

5) llD

.J.5.~ '?fb,-)° e.. l l()..\\

02.

Sample alkalinity (B} Mc;isured spike vnlile (MV)

% R = MV l SV x 100 (mgC:iCOJIL)

MV=A-B' (acccptnblc range (mi.: CnC03/L) =75 to 125%) ~-1 61 102-iJJ, S I amp e measure111e11ts: Sample volume Begin ll:nd Totnr. Alkalinit)' . Sample number S:imple ID. (ml) ml

ml ml Mtiltiolier (mi? CaCOJIL) o-4-15.('\\-

{)1l-)5u.J )(JO ':13.:-5" ~-3 6-8 to~t 5'l m 'ii.sw IJ\\l ) ?l1.~ ws,z. '5'~e CS°r a1. o'\\o*\\"'°1

04. ill-\\""'\\

2 £).t\\ s.a '5.6 @=\\ er\\ - \\ L.-\\_..., 3 '5.6. U.":\\- ?J,0. <.JD

er\\. ~'",:,-l\\

y II.+. r~.lJ' 6.0i lt10 "-"' en \\ "\\.* o \\.\\f\\atn \\ "nd g 2_ l~~l.t.;'l.1'.; ~-'1 l::b) '2.QOO* ~ HKu:* D02-I 5'tl 11,c; !'A?iL t, 1-lz") 3H n tr'i J(h U1- . l I()"\\ \\'1.4"1 2.. ',.,, *2. '#J. J

?q I

561 nr.n1<.\\. Yo : 3 ~~.l Ul.'i"', 3.i.:I j ~ C.OC} Reviewed by: Date reviewed: [ O"'\\*'l.\\*\\\\ Page 54 of93 SOP C6 - SxhibiL C6.. I, revision 01-01-13 ~ i.**:' '1f1~ I '\\-.------ -* fnV"honrncrit~fTe.stlngS1ltl!Oon,,,inc Analyst I \\)._, Date analyzed.1---Ll).~~-l-.-\\)-:--1 Alk:di11ity (SM 2320 B-1997} Mattix: Water, RL = 1.0 mg CaC03/L Titrate samples to pH=4.5 S.U. Pag~ 6'1 Page '2-of L\\ Time initiated t: n~ll-Time completed ~ I 1*c l

1/rfmf 1wr111a l(J* flJI[, 11111 t1111er ~ etermma/1011:

.nH or Normality Normulity (1\\~ of H2SOJ pH Fnctor or Multiplier Deionized Titram Begin End Total -(S ml NniCOJ.x 0.05)/E (fl/ x 50000)/ too ml snmpll! water reforenci: .standard m! 0.25/E Nx500 4.5 s.u. number number fE) (acceptable r, h ' 11 RO

0;0220)

'iJ..11-Y L b l I a OT:lllOTJ' COlt/ro SfOIJt ilrt.: Reference standard True*vnluc

Sample 1\\lknlinity (MV)

% RS= MV/TV x.100 number (TV) volunic Begin End Tot11I Multiplier (mg CnC03/L) (:iccep1:1blc range ,(mg CaCOJ/L) (ml) ml ml ml =90 to Il0%) l N'55\\~::, 100 100

~s ?A.2. ct.r Jo.,

'4-8 Cte.If). D11p/icate sample precision:. Snmple A!lrnlinity %RPD= ~ample Snmple ID

volume Begin End Tot11I Multiplie1*

(mg CaCOJIL) {(S - D) /j(S+-D)f2]} x IUU num.ber f-.JA (ml) ml ml ml (ncccpta blc range-== 10%) Kl\\: DoZi '5T i t().j

i. '-l s z'-1

~.. ~.~ -~ ' 11 lr'1I0.G"'i 3ll.Z. 41.~' to.1 L DiJplicnic (B) .) t.\\ l.l.p t.N~o 2."f l D Z'-{ ~<n..2111-Jilli atnx smke recoverv: Reference stnndard Sp~lt.e value. Sam11lc Spike alknUnity (A) i1umlier (SV) volume Begin End

Tohll Multiplier (mg CaCOJIL)

(me CaCO.i/L) fml) ml ml ml \\l.J~.~: '50 ({)..) 4\\-t,o 41.o +.~ \\ t>.1 -=1-'5" Sample alkalinity (B) !\\'lcasureil spike v:ilue (MV) % R~MV fSVx JOO* (mg CnCOJ/L) MV=A-B (ncccptnblc rang~ (mg CnCOJIL) ,,,75 io*l25%) 2~ 6l lfYZ.O ~/. s l I. amv e mettsriremeu s: Snmple volume*

Begin.. End Total Alknllnity Sami1lt!"nu111ber Sam rile ID (ml) ml ml ml Multiolicr

.(mRCnCOJ/Ll l1~\\-i.,.~.Q 1:1\\1 /.\\ K lf' b02 I 0r -z. 100 0.0 ':t. I ' i.t. f iO.. l L.J\\ J 4A i'1 (r\\ l'-\\. "b 1 3 4.l 0& 4.e \\ "'\\ t)'.\\ 1 (). <fl-. "fVA \\i< 1rOOJ\\J\\l \\* 50 . q,2.. Ill ~ \\.+. {i\\ aLl ,., en 1-i.\\1 I '],. I

<f0.'1

\\3.. e 2.'l I 5q n 0\\1'-\\. :oi..c ~ 3 1 t-3.s r:>.1

3,;;, r lb'4-n en, l:l
G:::l rKJA tu:- 002-I rJ1VV.l

~oo .f:}.\\ tt:f.1.I ~.?)

2. ?:>

l1 (r:1,\\.., * (,.\\ P:> -z. ' lll.1.4 ~~.; ~.l '-\\ '\\ .,mt~. ~1 3 Ji.3. £ :18.3 4.A 1..(e \\Hr1 n.. 10 If\\! A euroo'l- '

t:;D 0

2.0*~ :.e.e 4S i..."2.) C11 Reviewed by:: Date revie\\ved: j (T\\. '2..\\ * \\ 1 Page 55 of93 SOP C6 - Ex11ibit C6. I, revision 01-01-13

Analyst I IA.__,

Date analyzed*:. ~~J)~*:--'--~=-l,__:-1"-""l-= 1=== Alkalinity (SM 2320 B-1997) l'vfatrfx: Water, RL = LO mg CaC03/L Page* LJ?O Page* __ i_*-'. of_4 __ Time initiated I~ Titrate samples to Time completed:~~ pH =4.5 S.U. T/ 1tra11t. uormalit11 mu/ multio fer de1er111iu<<f(o11: pH of Normality !'lorrmtlity (JV)'ofH 2SO~ pH rnctor ur i\\'fufliplier De1_

-~

check Begin* End Totnl (5 ml Na 2CO~ x 0.05}/E (N x 50000)/ JOO ml sample wnter reforence -sfandnra -ml 0.25/E Nx500 -4.5 s.u. number number (E)

.(accc1...,.
~:...... lJ',..

0.0180- 0.0220). ...;-e11-u rr l b I I l a oratory co11tro.stem< arli: Reference standard True value Sample All\\nlinity (MY)* % RS= l\\'lV /TVx 100 number (TV) volume . Beoih Encl Tolnl (111 ultiplier . (mg CiiCO;/L) (ncccptnblc rnngc {mg CnCOJIL) * (ml) ml nil ml =!JO to*ll0%). I ~55'~~ 100 100 3Z<3 ' 4'l-~ ~*8 \\0.\\ C\\°\\ '\\C).61. D: l" I up tcate samo e prec1.wo11: Snntplc Afkafinit.)' %RPO-- Sam pin Sample lO volume .Begin End Total Multi(>licr. (Pig CnCOJfl.) {(S

D) /((S+O)t21} x 100

.number 1../A (mil .1111, ml ml (~1ccc.p1<1blc r1111gc. "'"+/- 10%)

1-i. tr\\l?..O'° {',i.J ~IY\\"2- -z..

6D t.\\1.\\.> t.ti.3 **1.1c::r ~ \\Q,\\' s Q5 '\\ Dilplicatc (8) .l O:o 4 ~tr l D q~ ..-..cJTZU:i- ..:./' Afi 1 atn:c spine recovery:. Reference* standard Spike value Sample S1>ike nll(alinity (A) number (S\\I) \\'Dhnne Begin End Totnl Multiplier (mg CaCOJl'L) (mi:CaCO.i/L) (ml)

ml*

ml ml \\~~l~'S~ Jf)O

'&)

D.O 'Mo, °'*"°

). lO.I l'lO Sample alkalinity (Bj Measured spike v:dm: (MV)

% R..-MV/SV xlOO - (mg CaC03/L) MV=A-B (ncccph1blc ra ngc (mgCnCOi/L) =15 to 125%) Ci6 q.; 'lS.67. Sample 111ensureme11ts: Snm'ple volume

Begin End Total Allmlinity Samnle number SnmnleID *

(ml) ml ml ml Multi1>lier ' (mg C3C03'Ll 1-i. U1 \\ s. o<a li\\.J A lu:r DO?; ~ 5U <i).'7 l4.<:; ~.ti rz.I 1at ~°' \\ \\. u-1' \\. t\\ 'r1'..J A a.u:' O/J., \\ l\\ll I tlw\\.s l't. ~ '.te C\\+ l~ ()"\\ l~.a"\\ I

z.

~.s 9.tt12.. l..\\:"t q~ \\'1 <n \\ s. U"1 J ..3 ~'{.z. ~q.. 1 '-\\.'\\ qq l ~ tr\\\\\\. \\ "L.. H"J A f'* 1 i:-ooi.n I rm, r *33.1-' 4.VJ q-, nn,~.c& I 2.- 3!i7-. ;5,;- 4.e '11. 1:1 Ol l ~. 0 e-l --3 'OB1'6. Ut~ Ip 6'. L

10'.?>

lDO 111n l\\.10

'rrt/ft.CW:DOZ Ov I Ll~lo 4'8,o '*L'-1. 0Qi cn'~.o~ 1 .z.. o.O L.\\ ::}* L.\\~'".\\.. 015' Reviewed by; Date reviewed: I 0'\\-1.\\-l "\\ Page 56 of93 SOP. C6 - Exhibit C6:.J, revision 01-01-13 ~ .Ew.1.lrol'\\t<<tmta.~Tl'.!o:.\\kt'l}-So\\u'Jon,Y\\~. Analyst I

'I),.,

I Date anal:yied* lf)'.-z.1.1-:r- _ Alkalinity (SM 2320. B-1997) fvlatrix-: Water, RL = l :0 mg CaC03/L Titrnte samples to pH= 4.5 S.1J. u' ~ ~\\-... Page J.tS J;

P.agc, _~Df_v_\\ __

Time initiated I==---~1..1 \\~ lime.completed ~ Tf r 1trn11t 11urma ity a11 d 1*c l . I' mt1 trp 1er < clermma 101r: -Ti ~T _ Normality Normality (N) of iliSOJ pH Factor or Multiplier Deionized Titrnni

l1ccll

.Begin

End Total

= (5 ml NaiCOJ x 0,05)/E = (f'/ x 50000)/ 190 1111 s11mple "'~tcr reference standard ml -O.ZS/E -Nx 500 -:.t.ss.u. ntimber number_ <E)

(acccp111~** *...,

,, 0180 - 0.0220) ,...t172{1~ lb I l n oratory co11tro stam 11rd: Reference sta11d11rd True value Sn1n111C Alkalinity (M") o/o RS== MY I TV x 1 QO number {TV) volume Begin End Totnl_

Multljilicr (mgCaCOiL)

(:1cccprn blc range -. (mg CaC03/L) (ml) ml ml ml =90 to 110%) _\\ Ns.\\\\GS~* 100 100 4Jr \\'-\\.~ C\\e> 10.l qq, C\\ctITT. D t tcp tcatt samo e nrecmon: Sample Alkalinity

%Rf'D.,..

Sample Sample ID volllnie Begin End Total l\\'lu\\\\i11l!~1* (mg CaC03/L) i(S - D)JJ(S+D)l:ll},\\ lllO .number --1"\\IA (ml) ml ml ml. - (ncccptnl>Jc r11~1gl~ =+/- 10o/~) Cu..t:uo-z.,

uu 3 5:0 {1) lO.\\

s~O\\' \\ i t5"\\ \\'$ -~\\o GD ll..\\..~ \\C\\~S' Hx:>

l.

Dupllcat~ {B) _} A.s 21.t.~ 5.0 4 J 0 1Ql, IDD -il if.r 2-1 I )-:- /l{atrrx*spi <e recm*ery: - Rcf~rence ~fandnrd Spikcv11luc Smnpll! Spike alltnlinity (A) number (SV) volum.c Begin End Total Mu It I plier (111g01CO.;IL) (rug CnC03/L) (ml) ml ml 1111 ~.~sS\\"S$o '\\DO eo A.S" (J.G,\\I' 04.lf l1.) 10.1 200 I 'Sample alkaiiuity (8) Mcasurccl spike v:1lue (MV) %*R= MV /.SV x 100 (mg CaCO;i/L) M.V:A-*B (:1ccept11blc rnngc (in!\\ C;iCOj}L)

/S lil 1'25%)

100 100 I CP.lf>. S I nmp e measnrei12ents: S:iruplc volume Begin End Total Allmlinity Snnmlc number SamnlelO .fml\\ ml ml ml Mulfiolic1* {ml!CnCOµL) l".. \\(!'\\\\\\. u "flf A r11i:-0021m0J). 60 ~q.~. :3~.z i.\\', ei.,,,,, to.1 q"":}- )""\\ lr\\\\~*cr:\\ 1

2.

3~.2 t1Q.-6 '4. lJJ 023. \\~lf\\ \\~.Oj .J .3 ?;de 43.e 5.. 0 lQ~,\\00 ,..., tr\\\\ \\, \\'-. ffJA rJ...U:"oo:unl)v l 1..1- '1 g1-s '-l, tA Q; ,.., (.)".\\ \\~ -~ l z;

~?.s l:tt:,?i '-1.e*

ct1- \\'\\CJ\\ 15,().£' l .J Lli,3 1.t~.:, cs.o. 1(;11

ICO

.,~ Reviewed by: Date reviewed:' r a-1-1,\\- \\ \\ Page 57 of93 SOP C6 - Exhibit C6. l. revision n I -ll 1-1 "l () ----= ~ -:=-::_:

, I I

'\\------- El'IYilQr'lm'!nl<ttTe.o;;tin9 Scfollohs;. In:. Analyst lr--\\J...J=----? D11te analyzed {(.T.;]._Cf,11' J Allrnli'nity (SM 2320 3-1997) Matrix: Water, RL -:* 1.0 n1g CaC03/L Tiln\\te snmples to pH =4.5 S.U. Puge-__ \\_of_Z. __ Time frtitiated r__...00:...J* u-3._2 ____ ~....j, Time complt;!ted JOO\\ '---'-=--'---------' 1traflt 11orma iJv a11 d l"l" d etenm11 n ttO!J :. mu tJp,ter pH of Norm:11ity Noi*mnlity (N) of ll~S0.1 11fl Fnctor or Multiplier Deionized Titrant ch eel< Begin End Tola I = (5 ml Na2CO,, x 0.05)/E = (N.~50000)/ IOO ml sample water reference st:lndnrd ml ml !Tll ""0.25/E "'NxSOO =4.SS.U. number i1u111bcr. (E). (acceptable nrngc = 11.0180. 0.!1220) L-{.<3 -IN(]..~ 1~~1se>'-i 0.1.fi \\?>.2 \\'2..;~ O.Olq6 C\\,q \\t c...+ o,ie-o.i.o.:. o~ rt\\ I Reference standard True value.- Sample Allrnlinity (!\\*IV) % RS= MV I TV x 100 number (TV) \\'OIUm!) Begin End T(ltal Mnltif)lit:r (m~ CaCOJIL) (acceptable rnngc (mg CnCO,/t.) (ml) ml ml rul ";'90to110%} \\~I'S$~ 100 JOO

13. 2. ;G.o q~6 c\\'R C\\1-cn-.61.

Dup{icate samv1 e nrecision: S:imple Allmlinity '%RPD= Sample Sm1ipfe*ID volume Begin E.od Totnl Multiplier. (mg CaCO,IL)

(S - D) ll(S+0)/2)} x Hit) 11111nhcr (ml}

ntl. ml 1111 '(:1cccptahlc range==: 10%) 1'3.0 \\1*2 cv1 s ~l o '1 *a\\.t. 1:t-fr\\ \\-\\~vJ tDu ~ct.2 ..l Duplicutc (B) ~

fq

i:

35~ 1.P*L ~ D ~I -.l1)z..~1J-1 'atnx. spt e-recoverv:. Reference standard Spil<e vnh.ic Sample Spike allmllnity (A) number (SV) voll1mc Begin End Totnl Multif>licr (111g CaCOJIL) (mg C:iCO.ilL) (ml) ml ml ntl \\ ~.SS\\~-a 5D I D::i '}f1*2.. 40:3. l L \\ C\\.O, \\\\0 Sample alkalinity (B) Mcasnr~d spil<e value (MV) % R= M\\l / SV ~ 100 (mg C:iCOi/L) MV""A-ll .(ucccpf:tlilc r:mgc (m~ CnCOJ/L)

75 10 125%)

Lo I '-\\'Cl Qf!;,ITT, s l amp, e me11sureme11ts: \\ Sample volume Begin End Totnl Alknlinity Sample number Snmole lD (ml). ml ml rnl Multirilicr (mgCaCO.;/L) lf1. a-1. \\t {Y'd-\\-~w too 4oJJ 1-!~;z..'5A 4.01 68 M\\tSlu UIJ \\ I 0,Q. (]"i* IS.*\\I Co.a (J. t} I 5ct en -'1.~ ~ I I \\ L (/). 0 1'2.o {0_0 6'1 O\\* 1:1 - ll J 3 12.0 r~.l\\ '5.ot cse "'~\\7..'-\\..c\\ 1-rJ A~~N*l 0 I ~ .~ I-+.~ 2l.-S- ' 3.\\0 (1) f I \\-:\\ 01 '?Jo.o \\. \\

z.

. \\,<; acs.o 3

s.

~ 'f ncn"'l~.~\\ J. 3 ~S.u 2(j;~ 3.s 00J \\I IJ\\"1.'.\\.01.. 'i'"J/i~tJIOt lrJ\\ \\ ~8.5 :;2.0 3~ lo&i \\ '"\\ Ot 1.b. en... ..\\ z 32.D.35.v 3*v,..

fl Page 58 of93 Reviewed by:

I~. --..+J+-\\-___,J Date reviewed: lro*-i. '\\-l1 SOP C6 - Exhibit C6.J, r.::vh~ion 01-01-13 I I ~ :t:::-~tal;.:ngS,ol:n:I~<;, Analyst I,}-:-- bate analyzed <fJrd..Ct;) r I Ailcalirtit)i (SM 2320 B:.1991) Matri;x: Water. RL = 1.0 mg CaCO;fL. Titnttesamplcs fo pH= 4.5'S.U. Page

2-of 2

.Time initiated.,j"'-==-- iii'-° r Tnnc completed ~ Tilrrmf11Qmmlit1* mu! 1ritilti lier deter1i:fmuio11: .Jlff Normnliiy Deionized Titrant wnter reference =4.5.S.U. number lnborafor11 co11trol stamlard: N&rm~l!fy (IV) orfl2S04 = (5 ml. Nn2C03 x 0.05)/E =0.25/E able range= 0.0180 ~0.0220} pH F:icfor ur i\\foltiplic:r = (N.x 50000)/,JOO ml sample =Nx 500 Reference standard True value Samjitc Alkillinity {MV) % RS = MV I TV x. 100 number (TV) (mg C11C03/L) \\ ~s~l';.:o~ iOO recisfon:,. AS:lmplC ID ~\\}.l'fk). ~ Duplicate (B) . 1vllltri.t. s ~Ike recover 1: ~Ci Referericc*st1u1dard ' number .Splice value (SV) (nig CnCO.i/L) olumc Begin (llll mt 100 ~...

l,Ip Sample

\\*of um~ ~cgi11 (ml) ml -~ 5b o~o j 3.s Sample, volume Bogin (ml) Jill a.J ~.s Smupic nll<nlinity (B) .. (ii1g C&COJIL) S I rm111 e measurements: End Total Multiplier ml ml LIS.~ L'.'.\\,a q_q* End Ti:itnl Multiplier ml ml

26.

5.S-C\\..ti

~-0 ~:'S \\ End Total Multiplier. ml , niL Mens1,frcd spike Yllluc (MV) MV=A-B <(JJ\\ CnCO:i/L) {mg CaCO,)ll_.) (ucccptnbli! range ,,,. 90 to ll0%}. q..,_ q~.D '/. Alkalinity %RPD= (mg CaCO~tL) {(S-D) /l(S+D)/21} x I 011 (iu:cq1rnhlt rn11gc=+/- 10%) > s [) ~'1 ~o~~n- ~pike alkalinity (A} (111g CaCO.i/L) %R:= MY l SV x lOO (:icccptablc range ""-75to125%) \\OJ, tf). .Sample volimie : Begin .End Tot:1I Allialinlt:y Samule number Sample ID "rill) ml

mr ml Multiplier (mg CnCO;IL)

'"" (l"'1'1..\\:\\ ** 0 \\ 1'JA ~lO\\U\\J ' f\\D 1~'*0 l"S.IO ~.!ti (t'\\ C\\°t -:}-1 \\.""\\Q""\\ '1,. lo

0.'

i '2-1-s.0 1q.1 3.~ I ~'1 \\ "ltt:\\7-fc*O \\ 1 .t .3 l~*I* ~~(o J:s LI.Cf I \\Cl-:\\1;,'ol,.U'L-fJ:A,SQ.¥\\.UCI\\ 1NfW \\ ila.y ~t,,.2 3.~ 1-'l .,, '"' en ".2.ic* IS'- .\\

i.

litJ.:;J;.. hA*S 3.v 1-' I l'"\\ ()\\ **t ~. 01 ] ..3 ~Q.0.33:'--' > 3.v. I ~ al ~ Date reviewed: I *01*'C:H1 I Page 59 of93 SOP C6-ExhibitC6.J., revision OJ-0!-lJ I I ti i*',{ l!iJ< *' (:~* l' -- __J '\\ ~" fflV°1fOOl'JWOf.'JITr~iif1~S9i~\\f~11s, Jn.t* Page z::i Page l of _:f Analyst I vCJ I Date analyzed A-UJ~\\r *. Hardness (SM 2340*C,.1997) RL = l.d mg C~COJL .:rime initia. ted I %~5\\ I T1111e completed* . 43:,: Ti. l'itrnnt Norm1\\lity* check Begin* End Totnl' No1'mnlity*(/V) ofEDTA pl-I 'Factor or Multiplier r~fcrencc standard ml ml ml =0.2/E = (N ~.50000)/50 nil sample number number (E} (:1cccptn hie r.auge = O,O 180 - Q.0220) =.NxJ0-00. \\]\\)~03 lv.J5S'-l8't o:o fo.o

o.o*

-rJ:o200 '"U)Q Lcrbontforp co11trol stamlanl* Reference st1mdnrd

Trui.: v:ilue Snmple Hardness (MY)

% RS = MV I TV x 100. number ffV) volume Bcgir E!ld Total ~1\\llti(llier (mg C11COJ/L) (ni::ce[ltn hie range (mg CnCO.i/L) (ml) ml ml nil =; 90* io HO%) ~ l l\\.15<::.1<00 40 50 .\\b~u 0-o -i...o 1£>.D L-{Q ~CQ.O). D1111licatesam1Jle orecfsio11: Sample*

t*lardness

%RPO= Snmplc-SamJ)le ID

voiuine Begin En<f, Tot1il' '.

Multiplier (!ilg ~n(j:Q;/l,) {(S-QI l!(S+0)/21} x*lllll number (ml) ml ' ml ml tf1',0r1- ~St}..) lQ'Q ' 1:2..o l~*O t.o -it>~o, *s.nJ \\ ~- Duplicate (B) j j\\.f.O tro.o "J.O Do_(;(.) -"CR20t'} i Reforence.st:ilidard Spike Vali.lc Sain pie Spike hardness (A} number (SV) volun1c Begin End Total l\\<lultiplier ('mg C11COj/L) (mgC:iCO.v'L) (nilJ ml , ml

nil

\\ t.,Jss. rsee 40

51) l'-l*O l6},Q.. 1..to. zo.o ea

_Stimple hardness-(~)* Measured s1iike value (MV) % R~MV /SVr.100 {mg entOJJL) MV=A~B (i1eceptilblc range (m:::.CnCOJIL) .,,. 75 10 125%) 40 '10 lQ9.6). s 'S: Samt>le volume Begin End Total Hardness Snmnle number Samote rD . (ml) ' *Ill!

ml ml Multlnller (mg Ca0J/l.)

TV=ND Blank 6b (}() 0*-0 'o~o z,.u.. n ktO (shoultl be ""0 mg. CnCd.:l!:.}. f-hS1'1-ifYl\\16\\AJ lAO '..ti'l.3 t.\\.3 r 0V C!"'\\*0\\;4\\ "\\ ' rt} \\.\\$1..0 u J j ' a~ '3 ~~.5 '"LZ.. ©4 C;f"'\\*lO*\\) )... ZwS 2>0;6 4.3 Sv 0\\-\\1.*\\~ 3 3o.e &1.lti Y* *.r ez. cr\\*\\~*1 ' - :. A Ll . ~YPt* ~'t Lt.3 6to ..z. \\°"'\\ er-\\ \\ ~

c.0-.

1 ~K\\P bo1-I

1s

'9St.z. .~o,z.; Lo l z.") '40* 0 \\-, *<nn A"1 I. .z yo;'J.. <<-t~I l.'l 7\\p n l1'\\ *.\\.* ~\\,.,. J -3 '412.rt c;!4.2_ Zwl 94

../L,.

\\,_ O""\\.\\O.(f1.:) t<it: O'J2..: ~-I- \\ ~'4.2 t.\\~,,Q;f!J 3z. Nol~; rr '>I Sml of litraut!S-. used. smnplc*mu~t be diluted: Reviewed by: I 6C I Page 60of93-D<Jtc reviewed I 6"\\*~*1"\\ SOP C7 - Ex!Hbit C7;'1. revision Ol*Ol-13 I ~--... ~ , Hardness (SM 2340 C-1997) RL = 1:0 mg CaCG3/L r; t f /.. l' I 1trm1 11orma t~p tl11l m11 '/10 1er c. eter111mat1011: Page 2.B r-~~e __.2_o f J* Time initiated r..........___ CJ1'~ Time completed ~ Titr:mt 'Normnmy 0 -oin End' Total Normality (N) or EDTA pH Factor or.Multiplier reference st1111dard in! .*m1 0.2/E' (N x 50000)/ 50 ml snmple number

number (E)

(acccptnu*",....., - "'118(1. 0.0210) Nx 1000

"7 Cf)-..DI:}

Laboratoni co11trof.stmufiml':

Reference stnmlm*d

'fruc vt1luo S:unpfo Hardness(MV) % RS*= i\\'IV IT\\' x 100 number (TV) volume Begin End* Total Multiplier (mg CnCO.YL) (acceptnblc rnngc (mg C:iCO;IL} (ml)* ml ml ml =90to lt0%) 1~~1soo 40 50 45 Lhb l;.0 i ~..,.a ~o ,1 oo~<D. 0 D l" I up, rcate samo e nrec1sio11: Sample Hardness l'oRPD= Sampfo -10 Am11icJD volunle Begin End Total Multiplier (ing C11C03/L) ((S- 0) /l(S+0)/21} '.\\ lllil number (ml) ml ml ml ) \\~61 \\'1;.s\\C. \\( l t:°Pf"9 ' iJ1 '2 2~ 5.o lti.O l10 ~ 20.u s t..lo

\\.

lSuplicatc (B) -4 °i-;,\\

l. \\

.\\ ..) D '-14 ct.s*1. lo.O 11: J ' aln.t *SPt 'e reco11erv; Reference standard Spike value S:irnplc: Spilce hardness (A) nu nib er (SV) volume Begin End To tu I Multiplier (mg.CnCO.VLl .(m~ CaCOJ/L) (ml) ml ml ml \\ ~5'~~00 f(y.::> 2..:; lo.O Q.2 3.1.- ri) 2).0 l3b Sample hnrdncs5 (B) Measured spike 1;alue (MV) %R= MV /SV i< 100 (mg*CaCOv'L) MV==A-B .{:1ccepl:1blc range (mg CilCOJ/L) = 75 (O 12,5%} '1'-l ~ tti::t.~'"I. S I amp e meas11re111ents: Sample volum!l *Begin Erid Total Hard.ness Snmnle number Sam rile.ID (mD ml ml ml Multinlicr (mg CnCO.JL) D TV=ND . J.should be 0 m~ CaCO.;/L)

lF;Zt.i~

,.., (j\\ l ~. ~.,i :tl}< \\t:°"001.\\}J\\,3 26 'c},z. lo.Oi '*1.+ 1,,gJ

tn.o lP-6

\\ -\\ tt'\\ l t>.tY"l) ;l'<"Kl t:' 002v'1 \\ IQ.q .11,C\\ 1-0 '-lo .\\ \\* O"'\\ \\-Z. * \\.\\., I ~ ~Ci

l~A

'2r0 e>o ,., en i-4* ~\\o ~ J .3 I l3.q i1.a.o '2vl Qi-{ .,,en io.~J ~'i\\ tF" 002 *.rJr vu*\\ Up.() L I0*1 o:r ze ,.., Cr\\\\1... AQ I

z.

!\\{.?;+ l+.e> La LIY \\\\ \\l"\\ '""' * :.\\ j ~ 1"1.8 \\C\\; (0 1.e °l'2 \\1 (l""\\ \\ \\. ) l) -(,)Ar11~ ooz.. t I IG\\.~ -ZZ.6 2.er lilo

IZ.o

., en l~.c)c.,,.

J 2.. 1-"2;"5 ~s.H.. 3.3 1 l.32..- \\3b

Notc:Jf>l3ml oflltraul is nscd.. satf!pk 0111~1 b~dilulcd.

Reviewed by: ~

Page 61 of 93

'-------'-1-"'----' SOP C7 - Exhibit C7. I. revision 01-01-13

l.

~ 1:*1. 't_ '

(' ~ '.'

I ,*--*~

- \\ £0v11unmer.t:ttTa'lilil'IOS0JmJor*r.lrt'*

Analyse I })-...> Date analyzed l!J}.Bio,n-- Tft t ll f 1

c l

1 rtm 11or111a IJ' mu 'lmt ftp, ter t eterm/1wt1oll.' Titrant. fljormmuy _* __ :

n,

Eitd-i-Iardncss (SM 2340 C-1997)

RI..= 1.0 111gCaC03/L z.q P:lge_.3_of " Time initiated I ~ cf.)1Dl:r Time completed: ~ Total Norrn:ility (N) ofEDTA pH !?actor o.r Multiplier reference

standard ml ml 0.2/E

-' (Nx 50000)/ 50 ml sample number number (E) (ncccpt:1b1c ntuiii: -{MH-Sll...:.0.0220)- -Nx 1000 'V :0"':!-ZOtt-LtcbontfOl'J' co/lfrol stiurdtrril: Reference standard True value

Sample
Hnrdncss (MY)

% RS = MV I TV x 100 nurnber (TV) volume. Begin Erid Total . Multipl,ier (mg CnCOJIL) (nci:eptnble range (mg CnCO.i/L) (ri1l) ml ml ml =90 to ll0%) lNSSrs*oo 40 50 i.:;,e '21-.0 /_o., UJ,o '40 .J(X)O) flp11cate samp e prec1S1on: S:unj>hi Hnrdncss o/oRP.D = Sample .......,... snmpfo JO olilmc Begin End

TM:1l Multiplier (mg CnCOJ/L)

((S, 0) /((S+b)f2l} x t 1)(1 number

1.'f A

. (ml) ml ml ml CL<.1= l:::fu '25 2.1.e ~. 'i Z.) -ze.o s l31P* I ':b* I °' tn \\'it,C>~ 3 5L2 ~ Duplicate (B) ..j '3Lz. ~tt.~ 3.~ l 1 D'~-\\YQ ---~ion .M t. a nx sm re recoi*erp: Reforcnce standard Spike \\'alue Sample Spike lmrdncss (A) number (SV) volume Begin End. Tofal Multiplier* (mg CaCOJIL) (mg C:iCOJILJ (ml) ml ml ml l~~l~OO . Bu 'ZS' 31.2-.,8/,,(o ~*~ "O* ~) z.o.o*. zz-o Sample hardness (BJ Measured spike vahie (MY) % R=MV /SV'x 100 (mg CnCOJ/L_l MV=A-B Cacccptnbh: ra11gc (mg CnCOilLl =75 to P5%) (\\..{() ~o. .lOO.OJ, s l <lfllfJ, e 111easure11u!llt.s: Silmjlle vohmie Begin Eiid Total Hardness Sample number Sample ID (ml) ml ml ml 'Multiplier (ml!.: Q1CO,iJL) TV =1~ Blank (should be -.0 mg CaCQ3/LJ \\-un\\\\.\\\\ ' 1\\J A Cur Doi i -Jf'\\ '2~ a"'*!O aq;2 ';Z.. ~,~ "tob f 01.f.. !'Co t"\\ (j\\ \\~.(I"'\\ l .2 3q,z. 41.'l.

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\\'ln\\\\,>n IJAw.rooi.vv t ~'}.4 ?Jo.Cl ZlO t20 \\'<n \\!:J. ob .\\ 'Z-2-0.4 34.2 3.*11 l3i.- 1.30 \\'l<S'\\\\S.ot:. ..1 -3 ~ ,,.t. .~t.'2- '3'+..'S* 3.3 I 1?12.- l30 Nore; If >1'51111:;if1itrm11 is oscd, snroplc must be diluted. Reviewed by: ~ Page62of93 '--~~__.._,.~~ Date reviewed.1 u'\\"to"i"\\ SOP C7 - Exhibit C7. l, revision 01-01'-13 I I I I ., r:--iL-;;:::J.*~* f.; I'.-- --.... ~ (*

-~....

I Page oD Page W of ~ 1 L _.., ) 2"n"1t<<lnment1ITit!nfq_gSfilutlo1a,htc:. Hardness (SM 2340 C-1997) RL= 1,0 mgCaC03/L Analyst l W Date *analyzea t--~..., ..,......-20-,l-')---i Time initiated E?~;- Time completed ~ 71" I 11ra11 11or1m1 1 11 "m 11111 IP 1er ~ eter111111at1011; Titr:int Normality cnec.- Encl Total Normality (N) ofEDTA j1H F:ictor or Multiplier reference

standard rri1~ hfi--t---!!!!.._

=0.2/E = iN x 50000)/ 50 ml sample numb cl'. number (El hll.'rarigc 0.0130 - 0.0220) N x 1000 .,;.<f1?Z)li-L b I I I I " orfl ory colltro stum nu : Reference standard True value Som pie Hordncss (JVIV) % RS= MV /TV x 100 number (TV) volurne Begin End Total Multiplier _(mgCaCOJIL) (ncccptablc r:mgc (mg C11COllL} (ml) ml ml n1I ='lC\\to \\10%) \\ t\\)6S \\Sd0, 40 50 31."S 3q;~. z~O 1.o.o ~o 1co.<1>. D l tip, icate samofe nrecisio11: Sample Hardness %RPO= Sample Snmplc JD vol11n1e Begin End Total . Multiplier (mg CnCQj/L) {(S'-.D) /i(S+0)/21} x 100 nu miler "'r-M (ml) ml 1111 ml l""\\.tn\\\\.\\\\' ~UFOO'ZhS\\\\k) \\ is 'bt3S ~IJ.' 2.IP ~) ?.O.o s JOL/-.IDO 1 .Duplic:11c (B) ..1 ~1.1--1* ~8.)- !li:.1.:4 j 90i. \\00 --\\l IA-:w.r-J 1ftlrl...: SPI e recover11: Reference standard Spike value .Sample Spille hardness {A) number (SV} volume Begin End Total Multiplier (mg CaCOJIL) (mg CnCOJIL) (ml) ml ml mi ... \\N~6,~e>e..

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z:i;- i\\o*l ao-r.... ~ ~) ~~o (QiO Sample hnrdness{B) Measured s1)iltc v;iluc*(MV) % R =!VIV I SV.x 100 (mg C:1CO.i/L} MV~_A-B (:1cccp1nblc range (mg CaC03/L) =7511> J25%J loo 00 f(X).ifJ S I amp e meas11reme11ts: Sample volume 'Begin End Total Hardness Snmplc number Sample ID (ml) ml ml ml Multiolier (mg CnCOJIL) TV=ND' Blank ~ * <f.}.?. on- .vJlul* G1.' :,.en rr~ Aw.Fooz,',.sN\\I :z.. 2-s io.~ 335 28 /"}\\ zo,o ll2.., llo \\"'\\<r\\\\c; *. 0'1 J ~ ?>:>. S'* :3\\o. 'l 2.~ .(

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Page63of93 .__~~~~-----' Date reviewed j cr\\*'lO-\\""\\ SOPC7-ExhibitC7.I, revision 01-01-13 " r--:ii-** -.-.. .-, I --* i_,_. .;;.'.J i. ' " i ~:-*

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fnvlronmenta1,TC!tff1!15clu\\fnn~.. 111c... Ar1alyst t.L,. Dateanalyzed I oi:t.g.Ci,n: Ti I:

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'Normality checl< Begin Encl reference standard 011 ml number number \\NUo'D I.>.}6S\\ '*t.£31-O*O ~-~ L<1!1omtor11 co11trol sta11ilard: Reference standard True vnhic

sample number (TV) volume

. (mg CaCOJIL) (ml) INSS 1686 40 so* Duolicate samn/, S:implci Sample Sample ID

,*olume number

-(mil u:\\-6\\lL.tl-. m H~vJ 60 Page 31 Page l of' 2-Hardness (SM 23.40 C-1997)

RL =. J,Q rng CaCOJ/L

'Time initiated f-Q~S5.

1 Titi1c compl¢tetl

()~ 2.$ Total Nonnali(y (i\\~ oHmTA pH Factor or l\\'ftl.ltiplier. ml =0.2/E = (N x 50000)/ 50 nil.sample CEl (acceptable l'angc = 0.0180 - 0;0220) =Nx.1000 l\\.t\\ 0*02.C2;. 1;.o.z. Hardness (MV} % RS"' MV ITV;" 100 Begin Eiict 'fotal Multipli.er. (rug 01C03'L) (ncccptable i::rrng 42.<1., L'l ~ \\ 10""'\\1.~*0\\. ~ J ~Pl l.'1 +l i..t'-L B \\""'\\ Ci"\\~'{

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}3 Note: If >!Sm! oflilrnm is uscd,*snmple must be clilutcd.

Reviewed by: I ). ( J Page 64 of 93 Date reviewed [-m01*1.C\\.. \\ 1. -, SOP C7 - Exhibit C7. I, revision O 1-01-l 3 ~ rd*-,.~,.'Q \\I I L.J \\------- -! {'1\\'ira11m~ritatTCJUng Solulif~nt."lnt. Analyst I t,>-- .:1 Date analy~~d===LA:'*=*=~=-~=-*=lt=*==- Hardpess (SM 2340 C-1997) Rl. = 1.0 mg CaC03/L Page 32. Pag_e -2..i_of 2 Time initiated 1= ~t-Time completed ~

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T." itrm1J ncrma ily am 1m1 11/J rer t. elermmut1011: Tltnm. ~

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=0.2/E = (N x 50000)1 SO mbmmple t-:---_ number number IE-) (:tee~... , -... uoc 0.0 I 80 - 0.0220) Nx 1000 r-tt.Y1-~lt Lilbornton* co11trol stnlld(frt/: Reference st:mdnnl Ti-ue vnluc Sample Jfardncss (MV) -%RS;;; MV /TV x 100 nunibel* (TV) volumo Begin End Total l'flultiplier (mg CnCO.VL) (11cccplitblc rnngc (mg CaCOJ/L) (.1111}' nil ml ml = 9(110 110%) l~~iseei 40 50 ll\\:~ ;\\(.l. \\.01 . 2o.2, ~e q2.s*1. D r I. lf{J, /Cale S({lllf) e f}Tf!CISIOll: Sample 1-End Hardni$s %RPD= Sample ,sampleiD volu.me Begin Total i'i'lultiplier (mg CnC0.YL) {(S -0) /((S+D)/21} x'IOO number K:°v'A (mil ml ml ml 14'0""\\'1.~.Ql ~MD\\~N "":i 2-S 0.1~2 :;\\3_'4 ?_ __ ? I t;i.") 2-Q. 2-s &:\\, .1 Dllplicate.(B). j ii ~ Zs.~ ~ J D 65 4.\\; *1, a.. 2~.l !vii utnxspr te recoveJ:11: .Rl\\ference standm~d - Spike value Sample Spike lmnlness (A) 'number (SY) volume Begin End Total Multiplier (mg CnC03/L) . (mg CaCOj/L) (ml} ml ml ml lA)~\\s©e 80, 2~ A~'i ii-s ~.it ~J '2o.2-. ri-o Sample liardni!ss (B} Menso.red spike aliic (MV) % R=MV /SY x 100 (mgCaCO:JL\\ MV=A-B (acccplablc r:mgr (mg CaC031l.) = 75 ro 125o/o) e~ ec::; IOa.-il. S I ltlllfJ e 111e1u;ureme11ts: Sam pfo vol ti inc Begin . End Tot:li Hardness , Samrile number SamoJcID (mJ} nll ml ml Mulliulicl" (mg CnCO;)L) TV=ND B.larlk

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Y.O J lbe'v- . _.,...L.1.,,, - "Lil*~ -.,_., 1.-1.1 ) Note; If >151111 ortitrant is used; sampf;m11s1 be dilu1cd. Reviewed by: I... r-. Date rev1ew~d I l .-1 0'.1- ~*rt Pa e 65of93 l)\\. g SOP C7 - Exhibit C7; l, revision 01-01-13 Page 66 of93 Sequoyah Nuclear Plant Biomonitoring July 25 -August 01, 2017 AppendixD Reference Toxicant Test and Control Chart Ceriodaphnia.dubia , Envlranm'cotal Tcsrlng Solurtcms. lrrc.. Chronic Reference. Toxicant Control Chart Source: ln~house Culture Control Limits (+/- 2 Standard Deviations) 1.08 1.06' --~~~~!-~.~-~~~~~~~-.;;

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1.04 I. 1.15 ... j......... _..,.,....., t....... ' *.,_........... *******'......................... 1........... 11'........... *t-*I.****** * *****..,........ Warning Limits 1.10 1.05 ---.-***~-*--*-**--*-**-**-***-**-***-**-**-*--**-**-**-**-****-*** e-e-*-- -*._cii_ C'I ~. --- * ~- *** -""* *--** -** **...._.. ".. _ .. f LOO ~* **! '.. -........................... ~........................................... -........ !'. ~.............. - -............... !...... -..... - -.......... ~ ~. - Test date 7-.day IC25 = 25% inhibition concentration. An estimation of the sodium chloride concenttatior;i whichwould cause a 25% reduction in Ceriodaphnia reproduction (calculated using ToxCalc). Central Tendeney (rnean*lqgarithmic IC25 converted to anti-logarithmic values) Con~rol Limits (mean logarithmic IC25 +/- 2 standard deviations.converted to anti-logarithn1ic values) L:aboratory Warning Limits (mean logarithmic IC25 +/- 2 coefficent of variations converted to anti-logarithmic values) USEPA Warning Limits (mean logarithmic IC25 +/- sA.io converted to anti-logarithmic.values, sA.ia = 10 1n percentile ofCVs reported nationally by USEPA.l age 67 of93
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0 -co Test number Test date 7-da"y IC25 ToxCal Determination 7-day IC25 (g/L NaCl}
1 03-08-16 1,0690 0.0,290 2 04-05-16 1.0720 0.0302 3 04-05-16 1.0627 0.0264 4 os-io~1s 1!0605 0.0255 s 06*07-16 1.0538 0.0228 5 07-12-16 1.0579 0.0244 7 os:o2-16 . 1.0662 0.0278 8 09-13-16 1.0623 0.0265 9 10-04-16 1.0758 (l.632J 10 11-01"16 i.0785 0.03?.8 1l 12-06-16 l.0753 0.031.5 1i 01-10-11 1.t\\123 0.0303 13 Oi-D7*i7 1.0641 o,021q 14 03-07-17 l.0641 o;ono 15 03-07-17 1;0614 0.0259 16 04-04-11 1.0654 0.0275 l7 05"02.-17 1.0691 0.0290 l8 06,06,17 1,0614 o *. 02s9 19 06-06-17 1.0518 0.0219 20 07-11-:17 1.0631 0.0266 Ceriodaphnia dubia Chronic Reference Toxkant Control Chart Source: ln*house Culture Log10 Conversion Anti-logarithmic Values (g/L NaCl) Litioratory Ca!cula~ed CV Cl s CT (.ontrol Limits Warning Limits er. :?.S CT+ZS cr~zcv CT+2CV 0.0281 0.0032 1.0668 1.0510 1.0828 1;0520 1.0815 0.0279 0.0029 1.0662 1.0521 l.0806 1.0529 1.0795 0.0276 0.0028 1.0656 L0520 1.0794 1.0528 1.0784 .0.0275 0.0028 1.0654 1.0516 l.0793 1.05211 1.0783 0.0270 0.0026 1.0640 1.0512 1.0770 1.0520 1.0760 0:0268 0.0027 i.0637 1.0507 1.0770 1.0515 1.0760 0.0268 ci.0027 1.0637 1.0507 1.0768 1.0515 1.0759 0.0269 0.0026 i.0640 l,0515 1.0766 1,0523 1.0757 0.0273 0,0028 1.0648 1.0511 1.0786 1.0519 1.0776 0.0274 0.0030 1.0652 1.0504 1.0803 .1.0.513 1.0792 0,0274 0.0030. 1.0652 1.0505 1.0800 1.0514 1.0789 0.0176 0.0031 1.0656 Hl506 . l.mios 1,0S:l,S 1.07% 0.0278 0.0029 1.0660 1.0517 1.0804 1.0526 l.0794 iJ.0276 .0.0029' 1,0657 1.0516 1.0800 1.0524 1.078Q 0.0276 0.0029 1,0656 1.0515 1.0800 1.0~24 1.0789 0.0274 0;00~8 1.0652 1.0516 1.0791 1.0524 l.Q781 0.0275 0.0028 1.0654 1.0516 1.0794 1..0525 1.0783 0.0276 O.ODF 1.0657 1.0524 1.0791 .1.0532 1.07?1 0.0275 0.0029 1.0653 1.0511 1.0798 1.0520 1.0787 0.0275 0.0029 1.0654 1.0512 1.0798 1.0520 1.0787 10th Percentile CV Warning Limits CT - SA,10 CT+ SA.Ia 0.9814 1.1521 0;9809 1.15i5 0,9804 1.1509* 0.9801 1.1506 0.9789 1.1491 0.9786 1.1488 0.9786 L1487 0.9789 1.1491 0.9796 '1.1499 0,9800 1.1505 0.9800 1.1504 0.9803 1.1508 0.9807 1.1513 o.~804 1.1509 0.9804 1:1509 o.~soo 1.1505 0.9802 1.1506 0.9804 1.1509 0.9801 1.1506 0.9802 1.1506 {'lore: 7-day IC:M; 25% illhibitlon cor1ceri1ration, An estimation of the sodium chloricle ~oncentratlon !llD! WO\\lld i:<iuse a 25% reduCUon ifl C~riodoplmio. reprod11ctio1i (carwlateo u~ing ToxCai(<), CT= Central tendent\\' or the IC~ valllei. S = Standard deviation oi th$ IC:;. lla!ues. control limits= Me.:in logarilhflli< re,,+/- 2 standard de*1ialions con1Jerted to anrHogJrithniic values. W;irnlnJl Uini~ = Mean logariihrn*~ 1c,, z 2CV or s:.,1a coo~erred to antl-logarlthrrilc '>'.31ues. SA.io; Sr.inoarti clevjat*tm corcespondlns to tl'\\e* 10 11 ' p~rcentlle of CVs repo!Jed na.uonally by USEPA (SA 10 = O.O.B); CV= Co2fflcienl ol variation.
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0 '- 'Q. w 0:: 'O c: 0 u '-0 40 30 20 30 20 10 0 50 40 30 20 10 Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: ln,.house Culture 0 <1*-- ~~~---~~-~--=e-~~~--~~~*-~~~o~--0*~-*-~*~~--0 ~-~ Q ~-~ --*- --- ---- *--- ~---- --o- ~-- --- --- --~ --~- --- ---- --- USEPA Minimum Acceptance Criteria (> 15 offspring per surviving female) USEPA Max*1m\\,lm Coefficient of Variation Guidance Criteria(" 42%), l\\ICDEQ Maximum(< 40%) ...__ ____ ~-- --** ---- ~--~ _.:..._~- -***- *--- --- ---
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USE PA Lower PMSD Bound (>13%) USEPA Upper PMSD Bound.Acceptance Criteria(< 47%) .--9:: - - *- -..::::..:m.=.::-- -*--e--. -.r - -=-=.~ - ** - - -.,. - -* - o - -.- -.--.

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co w Control Ceriodaphnia dubia Chronic Reference Toxkant Testing, Test Acceptability Criteria Source: In-house Culture ToxCal Determination Control Reproduction Control Reproduction CV Control Reproduction Test (offspring/(emaleJ l'i*J Test number Test date Survival Mean CV MSD PMSD CT 95%Corifldence Interval CT 95% Confidence Interval CT (%) (offspring/female) {%) (%) CT-25 CTt 25 CT-25 CT+2S l 03-08-16 100 29.4. 5.4 1;7490 5.9 31.2 28.7
33.6 4,7 2,1 7.3 s.a
2 04-05-16 100 29.3 6.0 1.7920 6.1 31.1 "28.6 33.6 4.8 2.1 7.4 5.7 3
04-05-16 100 32.3 4.9 1.6950 5.2 31.2 18.6 33.7 4.S 2.3 7.4 5.7 ~ 05-10-16 100 31;6 5.0 1.6360 5.2 31.2 2S.8 33.7 4.3 2.3 7A 5.6 5 06-07-16 100 27;9 2.6 l;731U 6.2 31.0
rn.3 33.7 4.7 2.ci 7.3 5.6, 6
07-12-16 100 32.5 4.9 1.6710 5.1 31.0 28.3 33.'7 4.7-2.. 1 7.3 5.6* 7 08-02-16 100
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6,6 2;1010 7.0 30,S 2S.2 335 4.8 2.2 7.S 5,7 8 09-13-16 100 29,7 5.9 2.0410 6.9 30.7 28.2 3B ~.B 2.2 7.5 5.7 9 10-04-16 100 28,9 3~4 1:6720 5~8 30.5 28.0 33.1 4.2 2.0 75 5.8
10 11-01-16 100 2s;7 4.4 i.8440 6.4 30.4 17.8 33.0 4.7 2.0 7..5 5.8.'
11 12-06*16 100 29.3 5.1 1.4550 5.0 30.4 17.7 33.0 4.8 2.3 7.4 5.8 l2 01-10-17 100 30.5 3.2 1;5240 5.0 30.4 27.7 33.Q 4.~ 2,3 7,4 5.8. 13 02-07-17' 100 17.9 8.5 l.8950 6.8 30.2 27.4 33.0 5.1 2.2 s.o 5.9. 14 03-07-17 100 29.7 3,9 1.5300 5.2 30.2 2.7.4 33.0 5.1 2.2 8.0 5.? 15 03-07-17 100 19.9. 5.3 1.9180 6A 30.2 27.4 33.1 5.. 1 2~2 8.0 5.9 1,6 04-04-17 100 30.3 4.9 1.9240 6.3 30,2 27.4 32.9 .5.1 2,2 7.9 5,8 '17 05-02-17 ll)O 31.0 5.9 1.8940 6.1 30.2 27.4 32.9 5,1 2~3 M 5.8' 18 06-06-17 100 30.7 4.9 1.9680 6.11 30.2 27.4 32;9 5.2 2.5 7;9 s.9 19 06*06-17 100 33.1 4.6 2,0500 6.2 30~2 27.3. 33.2 5.1 2.5 7.7 5.9 20 07-11-17 100 31.0 4.8 1.6610 5.4 30.2 27.3' 33.l 5.0 2.5 7.6 5.9 Note* Control Survival= USEPA minirl'lurn test acc~ptabllltvtrneila 2 ail~* >u:viv;;I. C>Jntrol Mean Rep,oducUon = USE PA rnlnimum test acceptability criteri<> '!: 15 0Hsprl11g/sorviv111z fen>c.le. CV= <:oefllcient of variation for to1111 oil eprc11.!utt1or. U5Ef'tl maximum GV ~uidance criteria (90 11' p~rcenlalej ~ d2%- NCOEQm;i~in1urn CVa"ept~oce criteria~ 40%, MSD = Minimum 'ignificant cliffe1ence, PMSD = Percent minin1um significant tliHe1ente. PMSD Is a me'asute of test precision. 1he PMSD 1s the minimum percent difference between the cont10Lano (f~atmem th>t can be declared statistically iig,nliicant ht* who!<? l!!fiuent toxiti!V 1e11. Lower PMSO l>ouno dete,mi'r.ec.I by US(PA'.(10'" pe*cen!ile) "'13%. The lower PMSD uound represents a.practical limit to 1he.~en~illvit.v of th!! test mellmd and b not a mlnlmwn*acc~pt*n.{e.r.ritNia. Upoer PMSD bound acceptllnce trilerln de1ermi11ed by USE~A (90'1' percentile)< *P'.'f CT= r~mral tendency o! the reproduc11o*n. r:.J or PMSDvalues. S = Slandard oeviat1on.of the reproductJ011, i:v~or PMSD villues. TestPMSD (%) .95% Confidence Interval CT-2S CT+_25 4.3 7.3 4.5 7.0 4,4 6,9 ii.LI 6.9 4,4 6,9 4.4 6.9 4~ 7.i 4.2 7.2 4.3 7.2 4.3 7.3 4.3 7.3 4.3 73
11.4' 7.. 4 4.3 7.11 4.3 7.. 4 4.3 7.3 1).3 7.3 tl.6 7.3
'1.6 7.3 lj.6 7-3
~ r:: '-r*, (7:1 SIJdium Chloride Chronk Reference Toxicant Test (EPA~821-R-02-013 Method 1002.0) Species: Ceriorlnplmia dubia CdNaCICR #: 204 Dilut/011 preparation i11formation: Comments: .NaCl Stock INSS number: INSS \\\\:;ti\\ Stoc:k pr~pnmtion: 100 gNnCl/L: Dissolve 50 g NaCl in "500 mL Milli-Q'wnlcr. Dilution prep (mg.IL) 600. 800 1000. 1200 1400 Stock volume (mL) 9 12 "15 1s* 21 Diluent volume (mL) 1491. 1488 .1435 1482 1479 Total volume (rnL) 1500 1500 1500 1500 1500 Test organism source i11(ormatio11: Test i11for111atio11: On?nnisiti:1ge: I < 2<1-hours old Rai1dori1izing lcmrilatc color: ri:e.~ Dale and*limcs org~itisms were bom I (!'\\-,,.,, Olo~tl "TO o'\\OS .Incubator omnbcr and shelf t.J)\\ between: location: Culture board: OHA.*O A Replicate number.* I ~ :t 3 I ~ I.s I 6 I 1 J s I 9 I 10 YWTbatch: a\\ *ti.*\\'"\\ Cullurc board cuo nurnbcr:* :. '° I l I\\\\ I\\~ Ii\\... I\\'\\ IU..11..~ l"b\\ Transfer vi:sscl information: pH= & * \\ "LS.U. Temperature= 'l,.J,.'\\ °C Sele11osm1m batch: O'\\*~-li Average transfer volume (n1L}: <0.25 mL Dni~p *renewal iilformatiim: Dny Dnte 'Test 'initintion *and feeding, MHSW t\\ nalyst. renewal :111d feeding, or l>atcli used termination time 0 07-11-17 Ci'\\ iS O"\\~ or.:.q x\\ 1 07-12-17 ('}~t.ls IS\\. Q I.-\\"\\ ~l 2 07-13-17 o~~ -z... en-~ "l"""\\ ii J 07-14-17 ot'-\\~ . trhtr\\'1
(
4 07-15-17 ()'\\_ \\~ crt* n..*n -j{ .5 07-IG-l7 6C\\\\~ .6'\\*\\!,* \\-\\. ~f 6 07-17-17 ogl\\4 tr\\.,.\\'9* \\~ 11 7 -.~**.. r ~.\\ 0'7-1$-17 C>&\\S Control i11fonuatitm: ~cccpt3ncc cfitcri.a Sm1inu11*11 of test endpoints: %.ofMaleAdults:
01.
520% 7-day LCso ") ILlOO % Adults having 3r<1 Broods; ICTJ1. ?.:80% NOEC 1000 % Mortality: n1. QO% LOEC n.oo M~an Offspring/Female: ~\\. Q
?.: 15.0 offsprii1g/fcnrnlc ChV ll11S.S
%CV: ~.&7. < 40.0 'Yo IC25 loL:.'D.\\ age 71 of93 SOP AT14-Exhibit AT.14.I, revision 11-01-lil
Page 2-of6 Species: C1triotlaplmia dubia CdNaCICR #: 204 CONTROL.
Sw*vivatalul Re111*of{rfctioll Dara Replicate number O:w 1
2 3 4 5 6 1 8 9 10 I 'i'oung pratlncc:cJ 0 0 D 0 0 0 6 () 0 n At!uh mortality L L. L \\_. L \\.._ L_ i Young produced ' {'\\ C". ('\\ ('\\ ~ 6 D () a 0 Atlu!r rnort:ilicy L- \\.... \\....._. L L '--* \\,._, ..3 Young produced () a D () 0 0 0 0 o* a Atlull rncirtaliry L- \\._. \\:....- L-.. '- \\..._ '- "L-L.. ~ Young produced '-\\ \\.\\ ~ ~ 5 Y. c...\\ '5 "'\\
'-\\
t\\cJull mortalily L. \\.._ L. ~ '- '\\...., \\__ L. 5 \\'oung (lrotluccd H v~ \\~,,_ tb .. \\ "'L; \\.b \\ \\.. \\""' I,...._ Adult 111or1nlity L L L \\,,,.... L \\._ L \\..._ 6 Vomig produced 0 0 0 (')' a ~ 0 c 0 {) Adult mortallt)** \\..._. L \\_ L. '- L... \\..... \\....... 7 Young produced \\.~ \\~ \\\\ \\ '-\\. \\~ \\ '--\\ \\\\: \\S \\'-\\ l \\.-,. Tol:il you11g.pr1,1!luced ~D ~tl ~~
~
~t) 3t> ¢~ ~1.. ~\\ ~o l'illnl Adult i\\'lortnlity L \\._ \\;_,_ '- \\..... L_. X for 3rd Broods \\'.'" "'£_ )<. ')<._ 1'-- f<.- J<-, 7-*. Nn1c: Adult monulilY (L =five, D = dc:ml). SO =split bniotJ.(singlc: brood split between nvo dnys), CO= Clll'IJ over (offspring cnm~d over wi1l1 ntlult dul'ing transfer). Cimcentmtio11: 'Yo Mortality: I
07.
Mean Offspring/Female: I 11\\t.o 600 mg NaCl/L SurJJiva/ <11ul Repro<l11cti011 Data Rcnlicarc 1111rn1Jcr Day. J 2 .'.\\ 5 G 7 s !) HI I \\'onllg 1irod11cccJ f) () f\\ 0 0' 0 0 n 0 0 Atlull morl:ility L L- \\,_. L \\.:_ L .L 2 Voung produced 0 0 a 0 l) D 0 0 0 'O Adull mortality \\._. L L L \\...... 3 Young p1*oduccd n {) () () () (') C) 0 () 0 Aduft,mortality L L \\._ L. \\._ \\,__ L \\._ '-
I
\\'oung ptoducccJ \\.. '-\\ '-\\ ~\\. s
1-,.
~ "-\\. ~\\ '..\\. Adult mortality -\\_. \\,_ L.. \\....... \\-... \\.._ \\.._ \\....:.. \\..._ Young produced l '.\\_. \\b \\~
to 11..
l~ 10 I °?-i \\~ ' {"\\ Adult mortalit)* L. L L \\.;._. \\._ \\.._, (j Young procluccd I"'\\ 0 {) 0 {\\ 0 a 0 a a* Aduh rn~rinlity \\._ L L L L L L L- 'L 7* \\'otuig produced l'Q l\\a
n.
~
.11 l~ l'l., 11... { t'S Total young, produced °'?) \\ ~~ ~'-\\ ""L. ~ :,u. ~\\ ~\\ ~~ &l '1..'\\ Fin:il Adult Mortality \\.....: L. \\.._ L. '- L-.. Note: Adult mortaliLy (L =live. O =dead). SB =split broad (single brood.split bctm;cr\\ !WO days), CO= carry over (offapring 1;orricd avc1.wi1h odult il1.11fog tronsfcr). Co11ceJ1tratiou: % Mo11ality:
01.
Mean Offspring/Female: ,";1,!, \\a % Reduction from Control: ..,,q7_ SOP AT14 -Exhibit ATJ4. I, revisio_n* 11-<H-l.tl
~ p !.. :..l /;:::J I, 'l PageJ of6
l
.'htl'l-o*u:t-.nlJlfdlfJ'll}W...:.....i.~ Species: Ceriodaplwitt 1ilibia CdNaCICl~ fl: 21M 800 mg NaCIJL Survival and Repl'0tl11ction Data Replicate number Day I 2 j 4 5 6 7 8 9 IO
1 Young produced
() 0 a a D () c 0 0 0 Aclnlt mo1*tnlit)' \\,_ \\_ \\..._ L L L. l_" 2 Young produced b 0 CJ. D 0 a 0
a 0
(J Adult lllortalily \\.. L L- \\__ L \\...... J \\'oung:produccd {') 0 0 (<) () 0 0 0 0 0 Aclult mortality \\.._ '- L "\\._ '- '- \\...... L-L L.... 4 Young produced \\,
'-\\
"'\\ s '4.. s ~ '-\\ ~ ~ Adult mortality * \\..... L \\._.. L L- \\_, '\\.._ 5 Young produced \\ l \\.~ \\""" .l \\ \\ 1... \\ 1- ..~ l <:::.i \\~ \\0
Adult mortality
\\.._ '- \\_.. ~ '-- x__ 6 Young proclucctl D r) (') lJ a c n n () 0 Adult morrnlity (_ L L \\..._.' \\._ \\...__ I...... 7 Young produced l I,,,. \\ '-\\ \\ \\c, '~
S I'?=>
1to \\ '-\\ 1'-\\* \\S To~:il young produc~d ':>~ "'!> \\ ~'l.. ~\\ ~~ ~~ 1.& !>\\ -==o Fih:ll Arl1ill Morlnlity \\_. \\....... \\...... L L. I.... \\._ Nole: Adult mortality (L =live, D =cl end). SB= split bro~>tl {sing.le.brood split between two clays), CO= carry over {offspring CilJTic.d*ov~r wilh odolt.r.Juring*lmnsfcr); Co11ce11trnlion: % Mmtality:
07.
Mean Offspring/Female:
~\\. "l- % Reduction from Control: -o.i. 7. 1000 mg NaCl/L Sm*i*ival mu/ Reproductio1z Data Rc111ic:1lc"11umbcr Ony 1 2
3.
s 6 7 8 9 IQ I \\'ouug ptoduccd 0 0 () 0 0 0 0 0 0 () Adult morlnfity \\...._ L l-L L L.'. '-- L L L l Young produccll (") 0 0 0 0 .. 0 0 0 0 0 Adult mortnl.ity \\_.. '-- 1-. \\.._ \\._.. \\_ il Young produced D t\\ D a 0 0 (') 0 0 Ci Adult niorlnlily L L. \\._. \\._.. \\..- '\\.._ L L L 4 \\'oung produced "-\\ 9 lo. -~ s '-l c...\\ I '-\\ ~ /\\d1il1 ruorlality \\._ L \\._ '- \\._ L 5 .Young produced I b ,,,_; 1.\\ \\\\ t'"L 1() t-z_ \\t_ \\.C J "L Adult mrmnlily L. '\\,..... \\._ L L _\\'oung produced D {) t'). 0 0 0 ().0 0 0 Adult mortality l-L L L L "L L-L L L-7 Young.prod need ~ I '1 J~ \\ '5 \\"~ \\~ \\b l '4 IS
12.
Total yom1g produi:cd "'1.""\\ ~~ ~<:) ~ "& 1. -i.,g.* l..C\\ ~() 1..~ "'~ 'Final Aduli i\\"lorlnlity \\..... I L \\ L \\._ \\._ L ,Vq1e: Adult mo11olity (L = live.. D = dcnd), SB= split brood (single-broad split between 1wo days); CO= carry ovcr (oflsprmg carried over wiU1 adult during transfer).. Conceniratiou: % Mortality:
01.
Mean Offspt;ing/Female: "2frt.(:, % Rt9uction from Control: .:.. s l. age 73 of93 SOP AT14 -Exhibit ATl4. I, revisi.on l 1-0 l-14
I l ~.. fl.:.;_-.::.-... ~ -7: : --- . ~~,.. Page 4 of6 Species: Ceriodaplmia rfubia CdNaCICR #: 20tl 1200 mg NaCl/L Survival mu/ Reproduction Dula .RepUc:.te nu miler Dar I. .2 3 4 5 6 7 8 9 l (I 1 Young produced ().
()
0 0 0 0 0 0 0 0 Adult 111orfnlity \\_.,_ '- \\.._ L L '2 \\'oung produced ("\\
O C)
D D ' 0 6 a 0 () Adult 11111rtalit)" L.- \\._ L-. '- \\..-'.......... J Voung produced 6 D ('} {') 0 0 () D r) {J Adult mort:ilit~ \\..__ \\_.. \\..,;;.. \\;_.,.. \\..;.....: \\.__.. J Young produced '3i ~ '-\\. ""L 5 ~ ?:>>.
"-\\
Adult*mo1<<nlit)I \\,_ \\..._' \\_
~ '--
5 Young procfuc~d 'S.
a ta "1...
'-\\. \\ ~ ' 5 q Adult mortnlif)'. \\_ \\..._ L. \\..._ \\- \\...... \\..... \\: (i Young produced D n i (:) /j 0 a c 0 () a 1\\dul! JllOl'l:llily L. '-- \\_ L .L L. l 7 Young proLluccd
4.,_
I_. ' S. ~.\\ '.\\ ~ .Total young produc~d \\~ JO \\\\ 8 '\\ ~ q ~\\ '1-l\\ . Final Adult iHort:llity \\_. '- L: '\\;_. ~ \\.- L. L Note: Adult n1orti'llily (L =five, D =-dead), SB =split brood (single. brood split bctwcr.u two days), CO c <.:arry over* (offspring carried over with nl1!11l-d11ring trnnsft:r). Co1tcl!ntrafioll: %Mortality:
07.
Mean Offspring/Female: lo. "l- % Reduction from Control: eo1.1 7. 1400 mgNaCI/L Sim,flral amt Ri!pi:oductio11 Data Rcolic:ilc number DR\\' [ 2 J 4 5
6.
7 s 9 HJ I Young produced 0 0 n* 0 0 0 0 0 C> 0 Ailult m1Jrtnlity L L L L \\._ '- L. L 2 Young. produced tr"'. c [\\ {\\ a 0 b 0 0 0 Adult morlality \\_ '- L L \\..... \\_ \\._ L J Young produced CJ
o a
0 £') 0 () (") n {') Adul1.mor1ality \\._. \\..... L L L \\;,,. .\\...... J Young produced* \\ 1-.
'b
\\ ~ \\ \\ \\. \\ Adult*morrnlity \\_. \\.._ L \\._ \\..._ \\..._ L \\..__ 5 Young produced 2.. 0 \\ D "'2.- : 0 0 0 0 Adult m*lrCO\\llt~* \\_ '- L. L '\\_ '- \\.._. \\_.. G Young produced a \\ 0 ?::i 0 \\ 0 1... 0 1\\dult mortalily-L L \\..... L L. \\_.. L L L 7 Young produced l 0 0 0 '-.. 0 () C\\ 0 \\ Total young prollm:cd ~ ~ b (,, s ~ ~ ::,. ~ 2-. Final Adult Morini it)' L L ~ L Note; Adult mortality (L =live, D = dc::id), SIJ =split brood (single brood ~plit bctwccn 1wo days), CO =.carry over (oflspnng cnrricd O\\'Cr with nduft during trnnster), Conce11tratio11: '%Mortality: Ot Mean Offapring/Fe111ale:
l..~
, % ReducLibri from,ControE &~. '1 7. /r~ ,.,,,...,,,. \\ ..... ~::.. *.Page *74 of 93 / SOP ATl4-Exhibit A Tl4. L, revision 11~01-14
Q) CQ CD ~ 0.... co (<\\) i ,_. --' f,___1 I I I \\ EnvlronnientalTe.sttng Solu~lons; Inc. C(lntrol Day Replicate number. 1 l 3 4 5 6 7 1 0 0 0
o 0
.0 0 2 0 0 0, 0 0 0 0 3 Q. 0 0 0 .o 0 0 4 4 4 3
~
5 4 4 5 li 13 . 13 ' 12~ 10 12 13 6 0 0 0 0 0 0 0 7 15 13 17 -14 15
14 17 Total
. 30.
30.
33 30 30 30 34 GOO.mg NaCl/l Day Replicate* number 1 2 3 4 5 6 7 1 0 0 ,0 0 0 0 0 2 0 0 0 0 0 0 0 3
o 0
0 0 0 0 0 4 6 4 . 4. ~ s 3 s 5 11. 13 13" 10 12 13 10 6 0 0 0 0 0 0 0 7 13 16 17 15 17 15 15 Total 31 33* 34 .29 34 31. 31 8QO nig NaCl/l Da*1 lteplicate nu'mber l
2.
3 4 5 6 7 l 0 0 0
o 0
0 0 .2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 .. 0.. .4 6 4 4 ". 5 4 5 4
5.
11-13 n 11 12 12 13 6 0 0 0 .o 0 0 0 7 16 H
16.

16. 15 13 16
'Total .. 93 31 3.; 32 31' 30 33 Verlficatio11 of Cerladaphi1/a Reproduction Totals 1000 mg NaCl/L B 9 10 Total Day 1 2 3 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0
3 0
tl 0 s ,4 4 41 A 4 4 G .12 13 10 119 s .10
12.. 11 0
0 0 0 6 0 0 0 15 14 16 150 7 13. 17.. 13 .32 31 30 310 Total
27.
33 30 1200 mg NaCl/l 8 9 10 'Total ciay 1
i.
.3 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 .. 0. *a o* 0 3 0 0 0
4 4
.t\\ l\\3 t 3'. 3 13-B 10* 119 5 s 3 6 0 0 0 0 6
O -:. *o 0
16 lll 15 1511 7 6 4 2 33 31 29 316 Total 13 10 I 11. 1400 mg NaCl/L 8 9 10 Total Day 1 z .3 0
0.
0 0 .1 0 0 0 0 0 0 0 2 0 0 0 0 0 0
0.
3 0 0 0 4 4 5 45 4 1 2 2 10 13 10 118 s 2 o* 1 (j 0 0 0 6 0 1 0 1*1 14 15 149 7 1 0 0 .28 31 30 312 Total 4 3 3 Replicate number ' 4* 5 6 7 0 0 0 0 0 0 0 0 0 0
0. 0 4
5 5 4 11 12* 10
12 0
0 0
0.
15 15' 13 13. 30* 32 28 29* Rep!fcate riumber 4 .5 6 7 Q 0 0 0 0 0 'O .0 0 0 0 0 4 4 2* '3 2 '4'
_ 1 3
0 0 .o 0 2 l 5 3 ,g
9.
s 9 lteplicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 .o a 0 3 l 2 1 0 2 0 ' 2 3 0 .1 0 0 2 0 0 G 5 3 3 8 9 0 0 0
o 0
0 4 4 . 12 10 0 0 . 14
15.
.-30 "29 8 9 0 0 0 0, 0 0
3.. 3*..
1* 5 0 0 1 4 11
12
'8 9 0 0 0 0 a .0 1 1 0 0 2
2 0
0 3 ~ 10 0 0 0 A i2 0 12 28 10 0 0 0 .. 4 4 0 3 11
10 0
0 . 0 1 0 0 1 .2 Total 0 0 0 44 112. - 0 140 296 Total 0 Q, 0 31 40 0 31 1(}2, Total 0 0 a 15 7 !l 4 35 /!-_~*....... ¥! _",,.. ~t;,~ ,..-~~
Environmental Testing SoJutrons. Jnc-. C911centratlon (rng/L NaCl) i
2.
Control 30 30 600 31 3:! 800 33 31 1000 27 33 1200 B 10 ~400
4 3
Dunnett's MSD value:
1.661 PMSD:
3 33 3<1 33 ~o 11 3 4 30 29 32 30 8 6 MSD= PMSD= Ceriodciplmia d11blC1 Chronic Reference Toxicant.Test EPA*821*R*02~013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Replicate number Sur11ival ** 5 6 7 8 9 10 (%) 30 30 3~ 32 31 30 100 34 31 3i 33 31 29 100 31 30 33 28 31 30 100 32 28 29 30 29 28 100 9 8
9.
i1 i2 li 100 5 3 3 3
i*
2 100 Minimum Significant Difference Percent Minimum Significant Difference Test number: ________ Cd-'._N_a_C_lC_R_<<_2_o_q Test dates: ,uly il-18, 2017 Average reproduction
Coefficient of Percent reduction (offspring/female]
11ariation* (%) from control (%) 31.0 4.S Not applicable
ll.6 5.8
-1.9 31.Z
s,z
-0,6_ 29.6 6.2 ol.5 10.i 16.S 67.l 3.5 33,7 l!S.7 PMSD is a measure of ti:st precision. The PMSD Is lhe minimun1 percent difference hetween the control and treatment that can ba declared statistically significant.in a whole emu~nt toxicity testi Lower PMSD bound determined by USE PA (10 11' percentile) = 13%: Upper PMSD bound rlelerm\\neil by \\JSEPA \\90'1' percentile)= 47'1'., Lower ancl upper PMSD bounds were determined from the 10th and 90th percenlile, *espectivel\\*, of PMSD tlata from EPA'~ WETlnterlahoratory Variability Study (l.JSEPA, 200la; US EPA, 200lb). The lower PMSO bound represents a practical limit fo the sensitivitv*ol the iest niethod and is not~ mlnimumcacceotance criteria. l)SEPA. 200la, 200lb. Final.Report: lnterlaboratory Yariabilitv Studv of EPA Shorr-ierm Chronic and Acute Whole Efthmnl To~icity.Tes\\ Melh[)ds, \\lolome~ 1 and 2-App.,ndix. EP;\\,82l-B'01-00tl and EPA*B2J -!!*Dl-005. US Envh'ClnmentaJ Protettlon A11e11cy, Cincinnati; IJH.
Start,Date:
111112017 End. oaie1 711~12017* sample*Oat.e:
Comments:
.Conc-mg/L D'COnfroL* GOO .wo 1000 '1200; -1400 1 30.000
11,000 33

000 "27.000 13.000
<'tooo* . $tatistltal J'\\nalyses
Cerlodaphnia*Sucvlval:and:Repl'OductfonTest*Repto>ductiori Tesl*I[): CdNaCICR

Sampffi: ID; Lab" 10:
EiS'EnVir. Testing Sol, .sample* Type;.
Pro.tD.c>.:>1: FWCHR*EPA:~21-R.02*01:l Twt.*Spe~lss:.
.2. .. 3 4- .s. .6 7 a* 30,0.00 33.,000' 30;000 30:000.* 3p,ooo
34

00.0 3.2.000
~3.()00, 34,000 2~;000 34,000* 3i;cioo
31~060 ss*,ooo 31.0.00 3;3,000
.32:cioo 31.000 M.oo_o. 33iOOO.2e:ooo 33,0QO 30:.0.0.0 30:000 32:Mei: 28.000 29.000 30.oO:o. 10,000*.11.006 -8,000 '?.coo i;!.000 9:000 11.000* 3.000. 3.000 e~ooci
S;OOO
3.ooo..
3;000 S:OOI)
RE:F:Ref Toxlcar,t NACL-Sodlum *ctlloride co-eaii~pnnia cub~
9 10 .31.000 30:000
fa.coo 29;000
-ai:.ooo 36,ooo
29:000: :za.ooo i2*ooti 11,000' 3:000
?..0()0 Transfol:Tll: Untransformed*
i:tan~*
.1-Taifed
1sotprilc

conc-m9/L_
Mean.N*Mean
Mean

Min:
Ma>:
c\\1%
N Si.im. Critical
Mean N~Mean o~conttol *31.000 1.0000 31.000

30:.000. 34.000*
4.809* 10 3*1..300 1.0000
600.' *31,~oo
i,Ol!;i4 31,600
~9,ooo* 3{000 '5;816 'Jo 117,00'. 75;oo 31.300 1*000,U
s_oo 31,200 7,0065
3~.200.28.000 33.000,
'5:190
to 113.50 75,00 31.200 0;9968 iOQO 29,6,00

0:9548 29:600 27:.ooo.
33:000 6.2oe 1l1 e.o.oo* 75.00 29.600* 0.9457
12.00*
10.200 0.3290 10,200. a.coo 13.000 16.5~5 10 55;00' 75,00 10.200* 0;3259
11100*
.3.SOO
o:.1i29
,3.~0<i 20_00 6.000 ~3:672 10: ss.Oo is.co 3.500 o.111a Auidliary*TestS Statistic Critical Skew Kurt. Kolmogorov 01Tes1*1~1~1es non-normal.dis_tribution*(p <:= O.o1). Bartlett's Test* indicates egual variances '!p = 0:83\\* H111>olhesis*Tcst{1*tail,*0.05) NOEC: L:OEC' ChV Ttl $teel's'Mariy-Qrie*Rank Te!!I 1 ooo 1200 ,1095:45
Treatments.lis*D-Control.
,,12066. 2.1681.1
Wnear*ln!eri>ola~lo!l.{!lDO'ResamP.les)

mg/L SO 96o/o'CL

Skew..
.Point
1,036 15:0863
!COS* IC.10 tc1s.
tC:io IC2!!
'IC40 ICSO .963;125 58,787<! 642:75 1009.57
4~1160 tOH.74 5,70623 *1062i69 1024,79 *O.Op6i' 1030.8_6 5.24817 102CM'i1
. *1040* *D,0731 1047,01 4,83636 1037.73 1055.42-
0.0806 1oe3. -14 4_4a3ss 1op4.39 1on:02 -o.oeao 1111.ss *'3.92798 M04.1 111~.'22. -0:oe10 114S:e1 4.01644 11.::16,04 11s1.. ai -0.0::13s
'hO-.-------------. ().9
o.a*
"" '0,$
g *o,...
. o *" ~ *o-4 a:: o.~. D.Z 0.1 ll (I 6--;...;...,-:-,,.-=c,.,..,,-(r'-
ll* ~ -t-.-.-,.-.--.-.,-,.-.-..-.--.,,-...-,....,.-1 Oose,Resp,onse Plot 3ri.
io.b---j---~-~-1

g

25 :.
~ 2 20, Cl. ~* 1~. 10' s' a o.~~~. ----cr----..,-----..-----.------1g. l* ~ ~ 0 Cl age 77 of93 .$qO .1000 Dose'mg/I;..
Used for PMSD calculation only. Start Dale: 7/1112017 End Date: 7/1812017 sample Date: Comments: Canc-mg/L D-Conlrol 600 600 1000 1200 1400 Conc-mg/L D-Controt 600 800 iOOO
1200
"'1400 Auxiliary Tests
1.
'30.000 31.000 33.000 27.000
t3.000 4.000 Mean 3*1.000 31.600 31 ~200 W.GDO i0.200 3.500 2_
30.000 33.000 31.000 33.000 mooo 3.000 N-Mcan 1,0000 1.0104 1.0065 0.9543 0,3290 Q,1129 Statistical Analyses Cer!odaplinla Survival and Reproduction 1est-Reproduction Test ID: CdNaCICR sample 10: REF-RefTO)<icam Lab ID: ETS*EnVir. T~sting Sol. Sample Type: NACL-Sodium ct1lorlde Protocol; Fl/VCHR,-EPA-821-R-OZ-013 TestSpei::ies: CD-Cerlodaphnta dUbia 3 4 5 6 7 ti 9 10 33.000 30.000 30:000. 30.000. 34.000 32.000 31.000 30.000 14:000 29.000 34~000 31.000 31.000 33.000 31,000 29,000 33.000 32.000 31.000 "30.000 33.000 28.000 31.0QO 30.000 30.000 30.000 321000 2~1000 29 000 30,000* 29.000 28.000
11.000 8000 9.000 a.boo 9.000'
~ 1.000 14.000' 11.000 3:000 6.. 000 5.. 000 3.000 3.000 3.000 3.000 2.000 Transform:.Untransformed 1-Taile.d Mean Min Max CV% N t-Stat Critical MSD 31.000 30.000 '34.000 4.809 rn '31.600 29.000 34.000 5;811] 10
0.826 2.287 1.1361 31.200 28.000 33.000
i.WO 10
-0.275 2,2$7 '1.661 2&.600 27.000 33.000 6.209 10 1.927 2.2.:ri* L6fr1 10200 8000 13.000 16.5:35 10 28.631 2.287 1,66'1 3.500 2.000 6.000 33.672 10 37.(~54 2,287 1.66! Statistic Critical Skow Kolmogorov D Test indicates non-normal distribution (P <= 0.01) 1.12066 1.035 0.25953 Banlett's Test indicates equal variances (p = 0.83/ 2.16811 15.0863 Hypothesis Test (1-tall. 0.05) NOEC LOEC CtiV TU IVISDu MS Op MSB .MSE F-Prob Dunnelt~s Test -iooo
1200 1095.4~
1;6!'122 q,05359 *f5$5A3 2-63889 O.OE+OO* Treatments vs D-Conlrol ~.:'~**;* l'ihlP"o<rtlf* t',.ll*v t.r~'"'"' V'-"*
age 78 of 93 Kurt
-0.5513 df 5,54
"FE"'"'=lq* ,..,,1--1* 1'.*-:- ~I I I~ ~ - ) &."'-im":,,~S-:.:~11:1..._ Species: Cel'fodavlmifl.rlubia J)aif:~r Cltemist1*y: Co11ce1itl'ation CONTROL . BOO nlg NaCl/L 1000 mg*NaCJ/L 1200 mg NaCl/L 1400 mg NaCl/L Param~tcr pH (S,U.) DO (mg/L) Conductivity
( J 1mhosfcni)
"Alkalinity (Ill~ CaC01/L) 4 ltarditcss (mg CaCO;IL}
Tcmpcraturc*(0C}
pH (S;U;) . DO (mg/L) Condncti\\'ity .(Jtmhosfcm).
Temperature (0C)
-pH (S.U.) DO {mg/L)
Couducii\\*iry J11mhos/cm)
~Tcmpernture (°C) pll (S,U.) DO (mg}L) Cond11cti11ity (J*mhos/cm)
Tcmpcr:itlirc ("C).
pH (S.U.) DO (mg/L} Conducti\\'ity
(Jtmhos/cm)
. *;.Tcmpemturc (°C) pH (S.U.) DO{mg/L). Conductivity (1-11111tos/c111) -
Tcmpcrnturc (°C)
Page 5 of6 CdNaCICR #: 2iJ:J Day (Analyst idemilicd for each day, performed pH, D.O. and conductivily*mcasurcmcnls onl1*;) ~\\ ~&\\ *.£ iaiJi-r.t.e l'-i?>O -,,~.<\\ 0.IJ\\.a !\\.8 11<1o "'\\..'\\.~'\\ re.oro q__g _r,\\ I lPD 1..'\\-4. e.os- '+.<3 ~S1-o "L'\\.~ O.a5 "\\-.°> AA60 [..q,'i Initial .o 1 2 -~ - *~1 :~----~ ---*~ ~. -.r 1_ ~,'!;...:_._.~*.::.~ I' L__ ~~ ..,~ (S.\\ U\\.~ 1.0in 800
. - ~* \\\\.\\'ZO
-=1-8 0.0 P'". *--.... 7190 e.o e.o e.o r--* -*~. I i, ** l." ,c, - ~ Fii1al Initial Final Initial 1-.e
~*---:,_;_--:-ro* *--
i * (:;-_ I ~* -* -........:.!. f. c_ -. --- ---1 'I..
1
~ r---.::::-:- -~*-: --*1 J -- __ :.li > <~C:~., '~ <:;-.. *':i 1 l**~-!~L,...: ___,:1 ~\\.'\\ fl*~-->"\\.~?~- - *. I L....... :1.:~.:..... "-"* -l,S.D I
f.

_ ___ "

_,,._,I.
Final ~Tcmpcrnum:s performed at 1l1c time o[ICSl initiation,_rcncwal ortcrminntion by the :m;ilyst identified in lhc Daily Renewal lnforma1io1i mble locntcd on Page J. Allrnliniiy nnd hardness pcrfonned by the an:ilyst idcr}lilicd 01\\ tha bench shccl'Spcc'lfic for each nrmlysis.and lmnscribctl lo this bench sheer by: age 79of93 SOP ATl4-Exhibit AT14.I, revision 11-01-14
Page 6 nf6 I Species: Ceri0da11lmit1 dubia. CdNilCICR ii: 20'1 ~j,_~--------------~------"T'""----------~-------------------0-ay----~--------~----------------- I l I I .l CONTROL 61)0 mg NaCl/L 800 mg NaCl/L lOOO mg NaCl/L I :mo mg NaCl/L 1400 mg NaCl/L pH (S.U.) DO (mg/L) Conclucti\\'ity (~m1hos/i:m) "illk:rlirtity (mg CaCOjfLJ '"flnrclncss (nm CaC03/L) "Temperature ("C) pl-I (S.U.) DO (mg/L) C*rnductivily (1m1hos/c111) ~Temperature ("C) pH (S.U;) DO (mg.IL) Con cl uctivify (11mboslcm)
'Tempera tun?
(oq pH (S.U.} DO (mg/L) Cor1d11ctivily , (Hmhoslcm) "Tempcrat11rc ("C) pH (S.U.J DO (mg/L) Co11ducrivits
(unihos/cm)

Tempera tu re (VC) pH (S.IJ.)
DO (mg/L) Co11duc1Mty "c1unhoslcm) Temperature ("C) Initial (Alialyst identified for cacl1,day. perromicd pH, D.O. anll conductivity measurements only.)
i. " -.
J Y-f40 Final. Initial -= r:.. I J 1., __, 1'52-0 ~- \\.... \\.1 . 'i),O I I. , 3no '1.S.c Final Initial \\460 l~O 13.0S L.S. \\ Finni luitinl
Tcmpcrntures performed at the time: oftest initiation, renewal or termination by the analyst identified in the Dnily R.znewal lnformnlion table located 011 Page I. Alkalinity and lim'dncss performed by the annlysl identified on the bench sheet specific: for each annlysis and lrnnscribed 10 this llcnch sheet by: -&---*
age 80of93 SOPATl4-ExhibilATl4.l, revision l l*Ol-L4 f._\\(-~.J
-**** f)
L ~~-;*:*-~,::~t~~- ;~
7.
'l Final
! ---- ~ *- Pimephales promelas Chronic Reference Toxicant Control Chart -u ~ ...I ~ -Ill N u - ( EnYttonnumtnl.T~ittng SOfuClon~. tl'1t'- Source: In-house Culture 1,0 0.8
o.6 '-
0:4 1.2 1.0 0.8 0.6 0.4 Control Limits (+/- 2 Standard Deviations) '°'--*---- _,_...,.. - --............... __ --*--F'°'-* -.--,,----. __ _ 0 o e e
--~-----8~
--*-----~------:"'."'~-----*---.-~ Cl 0 _.-------~*-* -- _..,,,_1 - 4'-""". Warning Limits ......................... ~ "............................. _,,.............. ~ - O e'" * '"' - <I o.-..._
o e 1
'< J " 1" " I o * *.. o ** * '" 4 o
t I
I ............. 1... _*I -- *"' - ~
_, __ 1' -
o -
* *- *

_.._ ~ *-..-.--.. o'
-~--. **-**--*-*** () Cl ___..~~~~~~~~~~~,;:._~~~~- e e ".~.. '°-.. I* - o _.._,._"'" .............. 4-* ** "....................................... '...............,.................. - Test date 7-day I~ =.25.% inhibition.concentratio*n. An estimation of the potassil1m chloride concentration which woulo cause a 25% reduction in Pimepholes growth (calculated using ToxCalc). Central Tendency (mean logarithmic IC25 converted to anti-logarithmic values) ~ Control Limits (mean logarithmic l[,_5 +/- 2 standard deviations converted to anti-logarithmic values) Laboratory Warning Limits (mean logarithmic IC,5 +/- 2 coeffjcent of variations converted to antHogarithmic values) USEPA Warning limits (mean logarithmic IC25 +/- sA.75 converted to anti-logarithmic values, sA.'7s = 75th percentile of CVs reported nationally bv USEPA) age 81 of93 J
v...... co Tlfanumber Test date 7-day ICzs ToxCal Determination 7-day IC;s (g/l KCI) 1 05-17*16 0.61109
0.1932 2
06-07-16 D,6998 -0.1550 3 06"14~16 0.7412 -0.1301 il 07-12-16 0.7.965 .0;0988 5 !>7-19-16 0.6348 -0.19711 6 08-02-16 0.7850 -0.1051 7 08-09-16 0.6703 '0.1737 3 09-12-16 0,7241
0.1402 9
09-1i~16 0.7351
0.1336 10 10-04-16 0.7791

0.1034 11 l,l-01-16 0.7808.
-0.1074 12 12-06"16 0,8303 -0.0808 n 01-10-17 0.7066
0.1508 14 02.-Ci7-li' 0.705?

0.1517 15 03~07-17 0.7620
-0.:1)81 16 04-04*17 0.7o43" -O.i523 17 os~o2-11 0.6740 -0.1714 18 QS-16-1? 0.6906
0.1608 19 06-06-17 0.7367
-0.1327 20 07-11*17 0.7519 -0.1238 Pil'nephales promelos Chronic Reference Toxicant Control Chart Source: In-house Culture: log10 Conversion Anti-logarithmic Values,(g/L ICCI) Laboratory Calculated CV CT s CT Control Limits Warning Limits CT-2S CTi-25 CT-2.t:V crricv -0;1573 0.0496 0.6961 0.5539 0.8749 0;4918 0.9530 -0.1571 .0.0468 0.6965 0.5614 0.8640 0.5026 0.9370 -0.1546 0.0451 0.7004 0.5690 0.8623 0.51-27 O.!l3i5 -0.1500 0.0460 0.7080 6.5729 0.8749 o.~172 o.9437 -0.1536 0.0459 0.7.021 0.5682 0.8674 0..51111 0.9376 -0.1502 0.0460 0.7077 0.5726 0.8746 0.5168 0.9436 -0.1517 0.04117 o.70Sl 0.5739 0.8664 Q.5190 0.9339 -0.1510 0.0433 0.7063 0.5786 0.8622 0.5255 0.9270 -0.1500 0.01121 . 0.7080 0.5831 0.8596 0.5315 0.9222 -0.-1477 0.0420'. 0.1111 0.5864 0.8638 0.5357 0,9254 -0.1456 0.0419 0.71!?"2 0.5897 0.8674 0.5398, o.92so -0.1423 0.0433 0.7206 0.5904 0.8795 0.5399 0.9411 -0.1433 0.0432 0.7189 0.5892 0.8773 0.5384 0.9392 -0.142;1 0,0426 0.7209 0.5924 0.8772 0.51126 0:9378 -0.1362. 0.0365 0.7308 0.6178 0.8646 0.5761 0.9139
0.1392.
0.0351 0.7258 0.6174 0:8531 0.5765 0.9012 -D;l37J 0.0316 0.72.92 0.6305 0.8434 0.5939
o,8858
-0.1382 0.0320 0.7275 0.6278 0.8431 0.590LI 0.886t1
0:1393 0:031/l 0.7256 0.6280 0,8384 0.5911
.0.. 8810
Q.-1393 O.Q314 0,7257 0,6280 0,8385 0.5911 0.8211 Nore1 7-day JC1 ~ =- 25% inhibition concentrati<1n. fin es1imatlo11 (lf \\!1e rio\\asslum chloride cancentrdtion that-would muse~ 25~: reduC!lori 111 Pimepholes gro\\vch jrnlr.ulated using To:i:Cak).
CT= Ce1wa1 rentlencv or the JC,, values. s = StanaartJ deviatiori of the IC,$ values-. Control limits= Mean logafll}lmic IC,; :t 2 StoiidJrd deviations converted to anli*logarithmlc Values Warning Limits= Mean logarirhmic JCi, :!: 2GV or s,..1, roriverieu to anti*lagarithniic values. SA.>*= Standard.dev1alion cotresponrllng co<he 75 1 " per1::en1tre cif c.v~ reportea nauon~llV by USEPA 1s, 1;"' U281~ CV= Coefiir.le11t ol variation, 7Sth Percentile cv Warning Limits cr-sA.75 CT+ st,.7s 0.4316 0.9569 0.4318 0.9674 0.4343 0.9729 0.4389 0.9834 0.4353 0:9752 0.4388 0.9830 0.4372 0.97911 0.4379 0.9810 0.4389 0.9834 0:4413 0.9886 0.4434 0;9934 0;4468 1.0009 0.4457 0.992.1 0.4469 0.99118 0.453:1 1.0086 0.4SQO i.00;1.s 0.4521 1.0063 0.4511 1.00110 0.4499 1.0013, 0.4499 . 1.0014

~-----*~

Pimephales promelas
' lml1'lmtlltnt.llTHliJtJ5eb:ut*n,1111
..c .... s: 0,._ (,!J 0
C 0 u
-e c 0 +:; Ctl Ctl > 0.... c QJ *u ii: QJ 0 \\J 1.25 1.00
ClJ, Cl!
a
~ 0.75 re ':p
c:
0.50 bO E a.is 20 .c ..... s 0 'l-. l!1 e .10 r:* 0 u... 0 - 0 30 20 10 Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture --- --~*- ~---*rs--- -*--;,

..,(lo----*

0 0
---**---1)*---0---o-e*----'o::......--*-o-n-*-
><~~ - -
USEPA Minimum Acceptance Criteria (> 025 mg per surviving larvae) USEPA Maximum Coeffident of Variation Guidance Criteria(< 20%) --*Jo-- __.. __ 0 0 0. Cl USEPA lower PMSD Bound (>12%) 0 USEPA Upper PMSD Bound Acceptance Criteria (< 30%) 0 ~ - --------------------*-~-*--~..--...-------- ------------ ~- Cl 0 G -~ *- 0._-. - --*,._,._..._,-- L-- -~ - 0 Control Growth, Coefficient of Variation (CV) or Percent.Minimum Significant Difference (PMSD) PMSD is the*percent minimum significant difference between the control and treatment that can be declared statisticallv signific;ant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is nqt a minimum acceptance criteria. . Central*-Tendency (mean Control Growtli, CV oi' PMSD) 5% Confidence Interval (i11ean Control Growth,. CV or PMSD +/- 2Standard Deviations)
Q) CQ CD,~.,, *:*"'.* ~ ~*""'""'* Pimephale~ prome1as ecs*.. ~ O~**------.- -..~r..-.~t.'-<. Chronic Ref~rence Toxlcant Testing, Test A~ceptability Critet:ia Source: Iii-house Cultu.re co roiica1 oe_ttirminat1cin Control Control GrovJth":
rest* number Test dare s.urvivaJ Meat'!
CV MSP (%):... . (mg/ln~i*l larvae)
{~}.
i 05-17-16 100 o.~07 1'._8 !J.0438 l .06*07,*16 100 o.* 42~ ~.o
Q.OS9i il 06*14*16 100*
~;~5 3.2 0.0{34 .a 07-12-16 lO~ o:s78 -ii.a* 0.0763 s 07*19.*1~ lQO* 0.774 10.1
0:1os::i 6
08-02.-16* lQQ' 9~570. ~_:., 0;047'1 7 08*09-i6
11101 0,699 3,5.
0.0827 B 09~ll-~~ ~11*cr 0;60_4. S;l ~.~532° ti 09*12*16. lQO o.631 4.)1 o.os2o 10 .* 10-04-16. i!)Q
o:see 3_,~
o.osos 11 11..01-16 lqo* 0.67l 7,7 0.!)~27
i2 12.-1!6-16*
iolr Oif1~7 s:s O.l167 13 01*1Q-i7 l~O .o;s~1 .7,;i 0.0629 ll'l 02*07*17 10(). D;!~79 n.s 0.07~1 15 03-07*17 100 0.638 6.2', 0.0662 i6 04*0~*17 19<j Q;620 5.S 0:991~* i? 05-~*17 100 o;7i!! *
o.o7Q2 18 05-16*17 lQQ J>,s93
~:s: O.OSll 19 06*Q6:17. 100 0.608 .i,.z* M>5112 20: . _07-11-17 . 100 10,iu: __ 3.3' 0.0589 Note:° C*;mtrol S~*,.j = l!SEPA minimum ten ~'!'eptabillty criteria~ 8!Y.G suri11va1., Conrrnl Mean Gr<ilv!h '." IJS8>A minimum le>l *c<<Jpt>blhl~ ci1tena ~ C,25 mc/!UMillni: Jirvne OI =.Ccolfltiont o{ vnrlliucn tor tonu<iJ ~roWI~, IJS'"::PA m***mum cv g*Jidanc-. tfll*tia j9o* percent11i!t.: 2m*' MSg = Mimmum 11cn11lcaii1 illlie10.nte1 PMSO = Pe1~t1t inm1mum !l&nilieant"dliferenco, '_Test Con~rol Gfowth ~ontrol !lro~th (V lmg/ini1;,1 ~rva!ll (~} PMSD CT
95% Confidericeclnforval.
er*

9.!!Y. c(!nfltle.iu::e Interval
.1%1.. cr-Z:s -CT+ 25 ci'-25 cr.,..2S 8.6 0:5966 0.418. 0,775.. 5,9 -1.0 12.9 i4.1* o.sni oim
.0.781
.s.s -0:7 12.4 7~3 o:sGa 0362
O.i73 5.6
,(J,~
12.Q
l3.2.
0.569 0.373 0.765 5.!l ,o.s 1£3
13.6 o.584 0.365 0.804 6.2
OA**
1':2.7 ~8.3 0.583 0:372 0.794 '6,0
O.S
i,is 1+"8 o.s!li 0.379 0.803
s;S-
.();~. 12.~ 8:8 0.592 it387 .0.797...
s;8:
-OA
ti,~
s*.2 o.s9s .0.395 o.i94 ~,1 -0.3 1W 8;6 0;594' o.401 0:788 5;6 .O;i1
u.s 9.3 o.s9s 0.!!0i 0.790
?.1 -0.7 1*J,5 18.9 0.599 .QA13* o.7s6 s:1 0.0 l~.4 10.7 o.~i)o 0.414' ii.is~ S.1
0*'1 1~,3 13.0 0.5~1 0.420 o.763 l?::i

0.3*
12;0 .10;4 0.S!lO 0,4il< 0.759 (;.:Z
0,11
.\\2,0 VUl" O.SQl (J..122 0.761 ~.3
o,6 1:\\,9 io.11 o.596 O./ll6 tJ:p&
9.2 0,6 ~~:~ 8;8 . o.~ b;4311. o.771 9,0 -o:i ns .8;9
0;601 0.43/l Q,768:
s.a*
_ip

l.Q~

8;a o.eo9 o.li3_8.
0.781. - . 5.6.. .0:5. 10.7 PMSD is a measut~ of.mt precision. The PMSD I~ 11'4 mlrlilTIUm per.c~litdllferenai bi!1wun theci>ritrcl an<l treatmenl 1foirca11 i>a docla~d tr~ifill~~Qy 11gnll1C;iu1In3 Whole effluent toxic!V !",!I. L1>wer PMSO bound de!cnnlned by ~SEl'A r16"0;Pl!ITI!n!llZJ;_; 12%.
Tni> 101vei:"M!';O bound repfe<enu a Practl(al llmli lo the ;ens*tiY>lycl tha luhMl~cd *nd I> ll<?t a minimum *~ep_!.$ncc rr>wi~ 1 UD~;,,1 f'MsO boom! ntt~Pl*nc~ trMrta determined bV us£Pll 190" iiercenlllo) !c 31/V..
CT=.CeJltPi terid*ncv<ilM gro>,\\1h, CV*or-l'MSDvalu.N; S: Sl*n~>r~ dP.y;~llc'I oi. lhe BtoWHr, Ol.or,l'MSP values. te.stPMSD (%) CT 95% Confidence Interval
CT-25 CI+2.S 11.3 s.o 17;5 n.6
,5,11 17;8 11.1 4;8. 17.6 U,4. 5,2 17.6
i_l.S s.s 17.6 11,3 5;2
-iz,11 ~i.4 s.s 17:~ l,l;~ 5.11 27;0 ~..l,0 S) 16.B l,Q,9. 5.1. 16.6 10.8 s:i. ~6.4 11..2 4.6: 17:8 11;2 4,6 i7.? lV'I
'l-8' 17,9 11.2 4.7 17.}
11.3, 11), i7.9 ~l.4 S;iJ 11:1}~ ll.4 s.* o i7.!) 1_1.p 5.2 16J~. 10.S. 4"
16.B-
~ .~\\
~ ff3 c:..:.;.-1 ~ ;~.:.'.! rf Q LI*- l
~
L_ . I Page I offi PoJass.ium Chloride Chronic.Reference Toxicant Test (EPA.,822,;R-02-013.Method 1000.0) Species: Pimeplwles proinelas PpKClCR Test Number. 28 Di"1tion vreparalion brformatio1i:
Comments:
KCI Stock JNSS number: INSS \\$q,°'\\ Stock preparation: 50 g KCl/L". DissoJ vc 50. g KCJ in l-L MiJJi:Q water. Dilution prep (mg/L) 300 450 600 750 900 1050 Stock volume (mL). 9 13.5 IS 22.5 27 315 Diluent volume (rnL) 1491 1486.5 1482 .1477.1 147.3 146~i5 Tolnl volume (nit) 1:500 1500 l:iOO 1500 1*500 1500 Test or~a11lsm illfonitatioir: Test i11for111ation: Organism.source: I n-hbilSC* cul tu re Randomizing template: U&riT CAA~ Age: < 24-hours old Incubator number and \\b .shelf location: Spawn dace: o, *o<; -,., ANemia CHM 1iumber: CHM914 Hatch dates and times: .n-11:1-\\"" \\~C)Q Drying i11formation for weiglrl it) 0"1-\\\\-n Ole ~s -determb1alio1i,* Transfer vessel infonnation: pH= 1,qq s.u. Datei Time In oven~ OMi*~ -~~ Temperature= 'l_{,i..1 oc .Initial oven temperature: ~-c. A verngc rransfer volume: <0.25,mL Date I Time our of oven: av\\'\\*<'\\ O"\\~S Final oven tefilperatu~e: 100'( Total drying time:
1.>\\-t~0&...S Dailyfeetlir~g.a11d renewal informntio11:
Day Date 'i\\*Iorni:ng feeding Afternoon feeding Test initintion, r~new:il, MHSW or lC?rmin:ition batch used Time Analyst Time . Analyst Tim<l . Analyst. O' 07-11-17 61.c\\l) H \\1-\\D \\,{ 0 g u::i 1'< cn-o.-..-n I 07-12-17 O'ooo .. ~. l"'l.bCl ~ O&C(:) M O'\\*M:::.~ n '2 ~ !ti v 07-,13-17 Ck>.bO ntti .nx. eo IX O\\*l{)-t'1 3 U" xi Q4db-ri 07-14-17 ' O\\oCD J'\\ \\ '1..Qc, \$ !'If d:O 4 ,\\J ~ u 07-15-17 ""' -:,~ \\l \\(""!IC ~ n~:=a...o 1-f ($".\\*\\"1.-\\\\ 5 07-.16-17 . v-ft Die '"=-tl x\\ \\'"L~o ,h OR '":>D CS'"\\*\\~,..\\} 6 07-17-17 nL6n \\( L 1:0{) .ti o.&oo IA Di.*\\:,-n 7 0.7-18-17 u1- . 0' '\\\\ 61 \\0 l Co1ilroi i11fon11atio11: Acccpronce criteria . Summanr of test endfJOinls: '%'Mortality: (', 1. ~20% 7-day LCso 8:"Zl.g-Average weight per initial larvae: 0.1\\~ NOEC kJaO .Average \\veig!1t per suniiving.larvae:. c.11~ l ~ D.15 mgllarvac LOEC -i<;c) ChV 1.o-10.8
IC~s
"'15. \\.. ~ SOP AT21 -Exhibit AT2 l~I, reYislon 11-0I~14
l
1 I
1\\.------ ~-3 ftt1'it:ln1";1"tnu.1Tu*ln9S.Cl~t10"1,f11c-Species:.Pimepha/es pmmelas Day Control A B.. c .D 0 ro !O [(I 10 1 10 JO (O ID .2 IQ II.) lO I\$ 3 ~u l10 CO IG 4 IQ I CJ ID It) .5 I() 'l{) I\\) lb (i ID l ti lD tD 7 I r::;., / IJ It> to A= ran weigh! (mg) Tr:iy color 5,0dc:~ fc:c.sf. ~ ,s:bi *~ 1-C{, 1\\11alys1: 'R!rJ ,~~o ~~o ~. IJ~tc: {j£. -I -)J ll =' 1'1111 +*Larvae. weight (mg) '?9-'>Ur. ~1>*~"' ~~\\ Analyst; ~ ~4.~ Date: QJ----l::1 '}. C= Larv:ic weight (rng) = ll-A lfancl calculntcd. ~ t...S'-\\ "1.:fi 1. 1.."!> i.~$ 1\\n:1IJst: Weight per initiarnumbcr ofl:irvoc (mg) = C / lnltial number ofl:irw1c .*v..: ,..,,J:? o'r flan<! ('alculnlcd. J\\. v "~ Annlyst: o* ~* o* o* Avcrnge weight per initial Percent
~ :'q l *.
uumbcr of lnrva.c (mgr rcduclion O.lt~ ) i rrom control ~
1-(%)
- _*';.~_'I -~. - Page 2 of6 PpKCICR Test Number: 2S Sur,,ival a1Ul Growt/1 Dara 300 mg KCl/L 450 mg KtI/L E F G H I J I< L 10 w IO IO 10 10 10 IO 10 IO ' I) ( 0 IQ to 10 10 to IQ (O 10 10 '° l () ro rt) IO to it) l(J co 10 lo l C) Lt) l C) /() I t::i. I CJ !(') to /0 l (j co fQ* l tJ /t) lO ID l.b io to l 0 ro tD Co l'O ro (fl I f::J l t) ID l'D l Q. (() ,~~t \\~~'& \\(.;;~°' \\'7£\\ ,~"f:>\\ \\\\sr:;f;,,~:t~ \\o.cP1 d-"5'i' '~\\~~ 9-o,,c; ';}. ' '}~'7\\\\ 1J.4:1$1 ?P\\ r°'°' gfl..~i '"1. O';::) j.ib., *1.'- 1.loQ '1,.~ i...'\\b ,.*t~ "\\.~\\ A.(? I\\'? I\\~ ~r:;, S'° °"~ A.~"t) I\\~ /\\. !'\\: I\\,; ~ o* o* o* o* o* o* I.'.)* ~* 0.1..:\\ \\ -;h°'..1. e.11.1 - \\,I\\ 7. Comment codes: c =clear, d = dead, fg = fungus, k = killed, m = missing, sk =sick, sm = Llnusually small, lg= unusually large, d&r =decanted and returned, w =wounded, Comments: age 86of93 SOP AT2 I ~Exhibit AT2 I.. I, revisio11 11-01-14
Page3 of6 Species: Pimeolwles promelas PpKCICR Test Numper: 28 SurviMl and Groivtlt Data Ifay 600.mg KClfL 750mg KCl/L 900 rng KC/IL M .N 0 p Q R s T u v w x
o.
JO JO 1'0 10 10 10 JO JO 10 IO 10 JO l /D JO to JO JD 10. ID q*-\\ IO '1 *c;l q'-< {'0 2 IQ ID IO It> Co I() /{) /.Q '\\ iO q . £,A. 3 l i) l~ IO ID 10 to IC) i() q
~
4 ~ 4 JD It:> lCl ID ID* 10 lD '..o\\...' ti:l ~G\\.. ~ (1) s 8 .£ 5 ID 10* lO ro ~* £IA. & ~"lA '2:~ ~ s ,g ~ s 6 .,J... ..,,.( 91'A '\\A \\~ ~ /{) It> '\\ ID g g g 3> 'i 3 7 .... ~". \\l..<t g .2l' '\\ lt::J l"b G\\ ftl \\ ~ 3 \\l..fp. A.., Pan wcighl (mg) Trny color code:: *~ore.~l ~2.n Annlyst: * ~ \\~~d- \\~ *'*\\l \\lo;9.l.D \\f;)$~ \\'3~,09'.e \\4.\\\\,~~'b \\~J:l>i \\5:19- \\c;,~o \\l.\\."(1 Date: ma-~~-IJ B = Pnn +Lanae weight (mg) .t;;.\\ ~~ ~~?- \\\\?t\\ Analyst: 1i1'tJ
~
~~\\!) rf_d:;;3 '}.'().'fl\\),~~o.. 'J.\\*Ul-1 n~°'
'\\~l.\\
\\\\,n;lt) Datt: Cf.1-~o-1:z* ~~~ \\.. '\\. .. c= Larvnc wei~ht*(mg) = B-A llnnd clllculntcd. -1.'b c ~-'°°' t...:~,.n $.~ 1$.~& i.\\.~\\ 't..~'\\ l.. ~~ '2,..U,. "t.sg \\.\\.::, Annlyst: ~ ',Wcight'(lcr ini!lal. number of hll'\\':1.e (mg) =C llriitinl number oflnn*ac 0-. 6' " ~ ~ ()'. v, ,.,,,rv ~ ~ \\.,SI c..,g. 0 Hand c:ilculntcd.. .~ -...9 "9 ~ t.; !)..,; rV' l\\r Analyst: o* o*
o**
o' o* Q'. o* 0 o* Q' 0 o* Avcrngcwcight per. initi:H, Pcrccnl l.i..'\\ 7. nnnibcr ofhirvac (mg) reduct.Ion o.lc.&\\ ~.. $7. o.s'--\\ '\\ (). 1.\\"1 1.,;c'.\\.io 1.
rrurn con lrol
(%) Comment codes:* c"" clear, d.=,dead, fg =fungus, k = killed, m =missing,. sk =sick, sm =unusually small; lg = unusually.large, d~r = decanted and.retumed, \\v = wounded. Comme11fs: age 87of93 SOP AT21-ExhibitAT2l.Uevision 11-0J-i*,l
l Species: Pimepkales promelas l'pKCICR Test Number: 28 Suri~ival mu/ G1*0111th Data Day 1050 mg KCl/L y z AA BB 0 10 1.0 IO LO l &T.~ 1.,'-l~,~. 'le\\ lo. 2 "2.<ll.
I.-
s'"" l c_ 3
il.'
w.. '2J'. I 5
'-l s
'-\\ 4 ~ ~ """'- \\ 3 5 ~ ~;>\\ '"l.-" ~ D ' \\ 6 0 \\ LJ,. ol/I, D J l .o 0 a A= Pan weight (mg) Tray color~ faresl *('F ec Analyst: ,~~(:\\* \\'ti.c.P'\\ \\i..JJf.,":i *\\1.\\:55 Date: Q{o*l~-"lf
13 =Pan+ Larvae weight (mg)
~,, Analyst:
')Yn\\
Date: 01-ac')-~:J \\~* C= Lnrw1c weight (rug)= 13-A llrual calculate~, --i... o.-n Analyst: ~ Weigh< per initilll numlicr oflnrvac (mg) "' C / Initial 11111nllcr of lnrvac D "'C\\ 0 Jfa111.J crilcuJ;ifcl.l. ~ Cl Analyst: o* \\ Avcrngc weight per inilinl 'Pcrccn! number onan*:ic (1!1g) reduction o,c'-°' ql:!l 7. from control (%) Comment codes: c =clear, d =dead, fg = fi.1ngus, k ;=killed, 111 = missing, sk =sick, sm =unusually small,. lg = unusually large, d&r = decanted and retumed, w = wounded. Commeuts: Page4 of6 SOP AT21 -Exhibit AT21.I, revision 11-01-14
l ~~f"n*Odktn t~(ll ~C! A*pJltlflfl * !!'utllrilt"lbfr.nf: f'l"llbUl1'lb!JC'. A~r~"*~1f111.fmc> l~l'V!_** lftWio_f.
p; 10
,,JO .IS.SO Caln~rD~ .. g.
10

10 15.SS c
,1Q !O 16.28
o
.10' '.10:... '.. 15.80*
10
.. 10 14.SS 3ou. ~F'
10
.. ur
ll.Gl
. :.G 10 .10 15.&9
If, 10 JO*
.. 1$.81 I
10
'10 '16.31'
4SO 10*

10 16.05 K*
10* 1a 15,76 .. L. 10 10 '_... lS.67. 'M.
10..
10* , lSJl2.* ~ .. ;N. 10 10* 14,47*' ,Q'. .10* 9<. 16;25 p 10* .10 1533.. a; 1,Q* 1
n.YJ 75U 10

1>
1.S.90 ..S. 10* 8* l4;ll T. .!ti' 8 JS.SB lU .. 10. 3. ' '1534 ~oa IJ, JD. .3. !5:12 w* lO 3, 15.H* .*.ic. .. 10 '14.91 Y'..
10.
O.
,0.00' lOSO* '.z. lO* ISM .M' -10 a CJ,00. 881 10 1 ' 'O' . '0.00 Ounol!l11i M50 '1alun: .. o.osii9. MSO= PM Sri,
83.
P~*"°*" Plmepltal~protnelas *<;;hr'ariTc Ref~renc;~ Toxli:ant terl EPA~S21*R,o2~(li31 Method ~QOO:~ Quality Control V'erlflcatlon*i;>f Data E11ti'y, Ciilc\\jlatlons, and.Statlstkal.Analyses. ll71'f.-.;Ltt¥H la~IJlf"'4l&f1tlm1)ll W1!1t.t I Survtvtnr-* Mtll't\\fi!*l&ht/~llMtffit Co*tfTde-1'11 or\\'!fr.,~... W*'*"'(lf\\ltt,l1tl411'b*1Ct w*f!tt*fm*r ~bo.J1t.rviill*('"ll numhraf,~M(rnd* OJ,*qw1..i~........... -, ,,,m_{'."d
,..;..,_**M"'**;>D*~
2z.a4, - *G:Sa*
Q.68q*
'3.6S.t ZJ.27' ,739, o.n9 Q:7H i.3 0,739* 2151 7:2:!. '0.121* 0.713 -i2,85 7.0S* .0.705 0.705 n.s1 7.lll o.1aJ. 0:703'
U:36
,7,]?,
0.77'!

li.7~i
.3 0:1"3 2295*
7.2* .. *o;m; ,0.726 2Ml .7.60* Q,760.. .. D.7GO. ZJ.st* .7,S6; .- 0,75<' 0.7S6
?!.01
.6.96" 0.096 Ii.in J~ D.6!16' 22.99 7.23 "0.723*' 0,72.l' .?2.98.. ' 1.n 0'731 0.731. .n.12* 7.30 .Q:7l0. 0,730. H.16* 6.69 0.669' o,6~~
1.4 0.669,,
22:.ll
6,07' 0674*
. 0.607 ll.SO* .7.17'
0.717

0.717' lt3'>

s.~1 O:rl1
\\),~'il';I, 21;88
5,93:
i:t.115.4 0.7llf 14.J 0598 .36.92 4.l!l 'il,60! OA61 2Ul: li.09' 0.761" 0.609' 17;49*.* 2.JS' o.n1 a.us 17!94 Ul* 0,740' 0.'11:?? iri,l
0.222 17.91 2.58*
0.&60 D.:?SS> 16.70 l.;13' 0,577' 0.173 .o.oo
o.oo, a.OOO*
.*--~ - -~- 0,000* 16:~1 0;77' o,no* 0.770 D.~ 0.077. 0,00 MO' o.oua 'o.ooo o.oo
a.co*

.o.ooo

o.ooa Mfiilmum s.r&niflcanttitrrerence fi*r~n* Mln!miom Sif,oiPi*ill Olfl!'f*nco TVttnymbet:. ______.,..,. __.,.....,,.....,..-1_1 To1tdo\\oi'..:..* _______
.;..Ju"-ly~*ll_* _*l_-8.;../_2* O_l_l. rill**l'l1u1.,;nJI~ Muntttl1h'1 tn11W ~mw.,,11tfttw:1iatb11 r*!"flt "d'!HIM ft~ml ""~~~11*r,.a !l;I C~rl~l~l l~f l"'ll
100.0 0:715
.u Not ~ot>llc>llle uo.g 0.1'1} u -3,!J ioo.o 0.n7 3,<i
ij
!J7.5 ,Q,t;U J.l 4.i. is.a
0.549
,-1i.i 2i.<J 3iJ;~ ~o.~11 16:1 l~f.~ u
0.1)19
~00.0 ~1.3
PMSD 1.i 0 n\\~3Stlte ~f 1en P'ect51Cm. Tti~ PMSO lS thO minimum perc~ntdiffer<!ntl! bet\\V..e~n t~11C1?rltr'?I ')n'1 h';'~.~111~n~ th.it =ari ~-~ ~~l~~~d.stall~\\li:tlHV 11c:11ill~nt In a Whole ~Hluf:nt tox;~ltV tad *
. lower PMSO boun4 oiir~rmli1.iJ1 Lv USEPI_\\ 11001 P."*<<ntll*I ;o *mi. .\\Jpp*1 PMSO'bound d~term1neiJ bv VSEM !90.lh po1<en111oi = 30%. tcwor ood uppt!t PMSO ~oUnd>were~clcflnlnod fr,om th..,lOth *nd 90th per<ontlle, rospoctlv*IY, ol PMS!> 4*to li~J!t EPi* Wti-(n!**loi>or.llOrl VarlabtlltVSttiW (VSEPA, 100 lo; IJ~liM, ZOO lb).
Tha fow.,rPMSD boui\\d tepte"enls i ~fyu;tf~;il ilmfl ta the ~cniltlvtwof the tl!tt mc-lhcd ar1;d h. f10t ~ ml11rqw1i\\.!¢11Pli1f1Cl;i ~rfl~rtii;

~,.
Statistical AmilY#es
S1arroa1.e; 111.112017 End Dal.ei

71ia1io1t Tesl ID: PpKCIC<R S:implEi.IO:
'REF*Ref.ToXtcanl Lab:r[): ETS*Enliir. Testtng*So1:. Sample Typo:, .JCCL-*PotoGsium ct1ioricte Pr.olo.ccir: FWC.HR*EPJl.r821*R*02*013 Te61*Specles. PP*Pimoplialas promalas Conc-m /L
1 2
,3, .D-Con1rol*.1.0000* 11(JOOO .1.0000 1.0000
300
. f,0000 110000. 1.0000. 1.ricioo. .4SO.toooo* 1c0000, 110000. -1:0000. EOO
1;0000* i.oooo 0.9000 i.oot.m..
750 '0'.7000* 0.:7000. D;8000 0.8000* 'YO fl 0;3000 *o.:moo* 0.30{)0 0.3000* 10f?O 0:9000 .9.1000 0.0000 0.0000
Transform:.Aresin.Squurc"Root**
Rank 1'-T.alfad Number Total .Conc.mg11:. Me:in N-Mean Mean Min.
Max
,.'C\\/"/o N .sum Critlc::il
Resp. Number.
D*C,qi\\lfol 1[000(,'I 1*0000'

1A1'20 1"<J120* *.M120. o~.oo.o 4*
)tJO l\\ilOOO

1.0QQQ 1*,4120 141~0' 1.4120' o:o:rio. 4* 450 1-.0000 1.0000
f,<1120 l.4120
'1;4.120' (),l)QO. ol*
GOO 0~750 0*9750 f.3713 l.2490, '1:4120'
'5,94::!
4,

o*
ia:oo 10.00 D 1B,O{l l0,00
o.
16;00. 10,00 1 '750.Q,/500 0.7500 J.0492' o,9912. 1.1071. 6;383
4~
'10.00
Hi.DO 10

9oo
U.3000 :iiaooo 0:5796:
0 57g5* o,57!is, .Q,000 4:
10.00

10.00 26
'1050 0:0250 0.'0250 0.1995. 0:15BB 0:32.18 '10.6~0
t 10.00 10,00
$!} Aao:Ulary Tests .Critical
Skow Shapiro'Wilk's Test inaicares non-nonnardlstribullan*Ci:*<= 0.01) 0,86999 0,69&*
.OE-14 Egualllj/ of variance cannot be confirmecl HypotheslsTest.(1*t:lll;'.0.05)
NOEC : l:.OEC.
CftV:. >TU Steel's Many-One Rank Test 600* 7.50
670182 TreatmenlS*\\/s D-Controt Maximum l.:ikallhood*Probit' Parameter.... Value.
SE. 95% Fiducial Limits, control Chl,Sq Crltii:al P.:valuo Slope. tritercept. TSC\\l is.aos;.2.os2es. 11.e435. 19;9299* o 0;9401;? 9,46773 *0;91874 ;i!,91.528 0,06'295 Point ECO!' EC06 EC1il EC15 F..C20 ECZS EC40 EC50 EC60 ec16 ECBO EC85 EC90 EC95 EC99 -41:214 B.02.62; *,s:t.t3 -20,.qs!i Probits .mg/L 95%'Ffduclaf.Llmlts* ' 2,574. 587/28 516.855 535.575 3.355 648.2A9 588.51 & 6S9A68' 3.716 6eu9a 6ao. t59 72Q,(l4~ S.9G4 108.01 65!.':ss2 74z.,e6e
USOl:,.,..-....,...,..,..
1 JOO 1000 10000 Dosa mglL 0 -~~---~£-.---.~o----.----,-----o..----+- m o 8 ~ ~ ~
0.
40' 40 ~o 40 dQ
40.
~O Kurt 2.67033 Iler
.3
-~-*'\\' .. ~ Start Datec 711~12017* E11d.D~\\e i'/lS/2017' Conc*m!:llL 1 o;cont!'OI* o;s84o 300 0.7Q30 450 0,7560 600.' 0;7.300 750 il:s;os.6 9ll0 0.2150* >ioSO 0,0000 2*
0.7390 IJ.?730 0.6960 0.6690 a\\ssl.io 0,2.220 o".atia Statistlc~l"Analyses,
- ~Larval Fish Growth:ai1d Survival Test-7 Day-Growth: '"(es't'IO: PpKCICR Sample: 10: REF-Renoxic-.ant lab' ID: ETS,Env1r. lesllng-Sot Sample Type! KCL,Polassium chloride
Ptotocol: FWCHR,EPM>2H'~'o2;fn3* Test.Species:
PP*Pimephales promelas.
3.
4 '0.7230' 0.7050 0.7.260 0]60\\l o.n:io o.73to o.eoro 0.7170 0..:810: i:i:6aeo
0,2580 0,1730' o.oboo O,OQOO'

Transform: Untransformed-1-Talled
tsotonic,
Conc*m£!1L Mean N-Mean Mean.
Min
Max.
CV% , N. o,con1r01 0.7128 1'.0000 '0.712~ 0.6840' '0;7390;' 3.;321
4 300 0.7405
'1\\0389 0.7405. :0;7030 0.1730 450 *0:7265 ,,0*193* 0,7265 :016960 _0;7560 600 0,6806 o:es51 0,6808 0,6079 *0.1300 7p0
0.5493 0,7706
'0.5493-0;4810 0.6090 ODO 0;2.170 0:30il5 *0.2170 0.-113_0 o.25ao. 1050 0:0193 Oi0271? o;qtes 010000: o.ono .Auxiliary. Tests. SllllpirOC.1/\\lilJc's Tes~ Indicates noi:mal dlstriblilion*~1=1->*0:01} Beltlell's Test* lndleales equal vanance.s (p--= 0:43) HypotheslsTest(1*tail;0.06).
NOEC LOEC *. ChV 01mnett'$ T.ist *
.soo ">600. . Trealmerils \\is D*Contiol* 4'308
ij 3:.402
-4 8,166 '4 1'1,622 4 16.065
l 200.0.00 4
Statistic
0.96535
i.111692 ru* **MSDu
0.0589'1

1*Stat
,1;~7'9
0:534 1,;;?4:.l MS Op 0.08265.
Point - *Lrnear.lnterpolallon (200'Resamples)' SD* 95%.CL(Expl
Sliew 431.7S 669,75-* CQ,"1430 507.55 i,;97,~5 *0.7644'*

Critical MSD Mean*
0,7266
two IJ.0589 0.7266 2:2riri 0.05(l9 0.7255
~.:t!99 o.osas
0,6BOB-0.5493 o.21fo
0.0193
Critical Skew

Oc844
-~o.5692 1.1.-3449 MSS. Mse*
.F..Prob D.00261 :0.00132 *0:1716 ICOS IC10 1c-1.s 1c20 IC25 IC4o ICSO ii6a:n

s:fo.56 67!2,00 713,44' 1a*1,93 B01.14

BSS.911 45:63
.30:26 25.0B 24,64* 18;96 9.18 6:84 579:B6 7Sf;~o *D.1.576. L\\l~-----'---;-=~---, sss.3:> .7B615._0;01so 0.9
674;59 7'05.48 '-0.7990 765-41 822:ss -'o,sfos o.e *

8Ci7.65 *849A7; -D.4666'-

0.7,
.. 1*
age 91of93
,fl>. 0_8 T
Ill
'.g_ ~-5 ~ _g. 0-~' I?! 6,3 0,2. 1,).1 !J.. a oh..,._,__... _ l)o. -.:!" a QOSC"ReSpJ)nSe Plot 0:9~---------------------. 0..,
0

O C*
500 io*ao' oose-mglL 1-'!all, 0;05*tiNel ~f.slgnrilran~ i5,00 N-Moan 1,0000 1:0000
o.999S 0:9369 0;7559
0.29B6

O.OZ65

Kun*
0.13191
df 3; 12
----/-
~\\ tn111r11nm'!nl.d'rt11~ Soltnfan-.lra:. ' Species: Pimeplwles promelas Dailv Chemistry: Concentration CONTROL 300 nig l,:CllL 450 mg KCl/L 600 mg KCl/L 750 mg KCl/L 900 mg KCl/L W50 mg l{Cl/L Analysl _Parameter pH (S.U.) 00 (mg/L) Conductivity (umhos/cm} "Alk:tlinily Ong C:tCOi/L)
H::mlncs*s (111g CnC01/Ll

Temper:ttu_rc (0q pff (S.U.J DO (mg/L)
Cond11c1ivily (umlios/ci11) PU (S,U.) DO (mg/L.J Concluc111'it1* C1-tmhos/cm)'
Tcmper:ttui;c (°C) pH (S.U,).
DO (mg.IL) Conducrivily iumhos/i:m)
Tempcrature_(~C)
'DO (mg/L) Conllnclil'ily (11111hos/cm) "'fcmpernturc ( 0C) pl-J (S.U.} DO (mg/L) Conductivity (1u11hos/c:111J "Tcmpernlllrc {"C) pH (S.U.) DO (mg/L) Conduclivity (lllnhos/cm) "Tcmper:iture ( 0C) Page 5 of 6 PpKCICRTesl Numbc:lr; 28 Day (Annlyst id1:ntlficd*forcuch duy;.ocrformdd pH; D.O. nnd comluctivity measuremcms only;) 0 I 2 H.S r*:r. " (
.,... __.;;.~-....
~-~-- 'l.'-\\. s 7.grp 4.6 7.-; \\ I I "-:'l.l{.!) 0.0S" 7-R J f -,",:::- -----.. ~ .;:..\\_.,, . I "P ~ ) L -.,.,:_'.*~j "1..-\\-1..c> 1.51-1-.1 '1. '\\.~ '"L -:\\.se "'1.z. -~ ~... : _J }o/O(l I c "- f,
..=.
6.0(0 1~10 0.oi:s {3.0 Initial
l
~ _., l..l\\.\\o r *-. *-- ~ -- ~'.......,.._.,:_ _ :_ -- .1 ll'inar 8.oo 2200 Initial I.'{. 5 i"'.., - *,. -~ ,, ---~ ;.- i Final' .--;"°">--:"*--~*- ---*"' ~-.. -* :\\u.:._;~:; ~i °1.'l. ~ ."\\..**\\,.'$ '1Ae '~.Sl '8.n 'f.3 -.. -- -1.
~w ' '
7~-5~ 1-*-----~' 1..1{.4 ""L'"\\* ~ '°}.('.\\":}. "::l.S\\o 'l>O '1.3 'AoO
~~~-.--*-- ~ -~-:J.!
".l..'\\. ~ '1>-\\..s .1}~8 ":.)-.5").. (p-p '+~ !i&Q)
r -.. ~_.-,,.-

,'*~~-;
-*-,, '2* i."{. e ..,,~ :1 Initial Final
Tcmpcralurcs perfom1cd il11hc titnc of lcsl initiation, renew~! or 11:r111i1101ion by the onalysl idcnlificd In the Dnily Renewal lnfon11atiol1 tablc lbc~tcd on Page l.
/\\ll:alinity and hnrdncss performed hy the annlyst identified on the bench shecl-spccilic forcuch analysis ;ind-lnlllscribcd* to I his bench sheer liy: --.1.61+----'* h1U*111nd1rnt age 92of93 SOP AT2l - Exhibit AT2 I.1, revision 11-0 l'-14
~ *1!:.1 ::::JJ Lt*::a (F 1 '=J l _,;-.. Page 6 of6 I
~-/
_ 1 Envlranmw1a.f1"sllr.9S-.Cluuans,lnc... Species: Pi11zeplurles promelas. Ppl<CICR Test Number. 28 Day (Analyst idc111ifrcd forcncll day. pcrform~d pH. D.O. and conduetivily mcnsurcruents only.) 3 4 Analyst *'tv .O:i=.v- ~t - .~~'- ~L..- CONTROL oo <mg!LJ A.o +.hr "3.c -=?*'-" f..5 JOU mg l(Cllf.. 450 mg KCl/L pH (S. U.) ~!) :}- -:1. lt1*'+ DO (mg/L) t::l:n °1-P\\1 600 mg (\\:CllL Co11dudivity l"1L1. O (imilios/cm) \\ v.,.
~~.-:.--* --:_~-r _.,
f.t
~~
<<Tempc~ntnre
...i (OC) ~'-\\*& pH (S.U.) ~P(.} '1.'1-D.. 'd.ol DO (mg/L) 0.n 'Y:.O 750 mg KCl/L ~ - --r.
.-;-~ ;, t L ~. ___.:_~ _ _.
Conductivity l...-*-:i.- (11mhos/cm) '-=:l -iu "'"tem pcm tu re , 1 f! (oC) I,.~. 11: 1.4.1 . pH.(S:U.) '1-.l\\0 "DO (1i1g/L) .8.0 'if.. o 900 mg l<CllL .I 'loo JO"'*. -"';?':-:- -:i .!,,{L:) ~: **- -.-.;. !i_! Conductivity l ~-:'.J:.* "..... (umhos/cm} t:::.J v
1'.Tempcrnture
-;,, c. (vC) L. "\\ * ~ DO (~ng!L) (J,0 IOSOn1g KCi/L Comluctivily . ~'\\ lO (um hos/cm) (!\\ r~ 7* \\.. '~?.' '"Tcmpcr:\\ture 1.l\\. ~ ("C) Initial Final Initial F:lnal Initial 5 , \\ '+.v5 i
,, '; *1.*
r I f;_-/ -::-: -..... ~ I :,,- ,,,.c "'L4. s --~:- ""?-:::~~: **r-.,~,. ~:"'.?;-;~*
i
----~.,) Finiil 0.o4 l\\ld 0.o -1°110 '13.0lo Initial 6 -*.~I ~ ----*. *-*-** -:....:~ ... ~ I r "b-\\. \\.\\ t --:------..... :: ** l J ---- ~ J r.- -.. '~:---~
.*,*.1-:-
l*-~ ;} * """ - 1J\\.S Final
, *~
mtlires perfonncu nHhcliflle ol'tcsl ir1itiation, renewal or.1cm]in;1!ion by the analyst*i~enlilicd in the Pail}" Renewal lnfor111a1ion table located on Page i. ,,u.~11;.il.ini and hnrdncss.perf.nmcd by 1hc on~lys1 identified on the.bench*::hcct spccilic for.each analysis and 1runscribcd 10 lhis bench sheet by: ~-' ~. age 93 of93 SOP AT21-ExhibitAT21.l, revision 11-01-14}}

ML17261A197

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