ML13289A199

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SQN Annual Water Withdrawal Updates for January 2011
ML13289A199
Person / Time
Site: Sequoyah  Tennessee Valley Authority icon.png
Issue date: 02/09/2011
From: Skaggs M D
Tennessee Valley Authority
To: Cromer P
Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
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Download: ML13289A199 (175)
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{{#Wiki_filter:S58 110209 801 -NPDES CORRESPONDENCE February 09, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor, L & C Annex 401 Church Street Nashville , Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JANUARY 2011 Enclosed is the January 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 1/29/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information , please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I cerlify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system , or those persons directly responsible for gathering the information , the information submitted is, to the best of my knowledge and belief, true, accurate , and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely , /] /! r / ' / 1/ -! c/-) ' .**"" .. * \ / .v* \ I I } ' Michael D.* Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure)

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building , Suite 550 540 McCallie Avenue Chattanooga , Tennessee 37402-2013 cc: B. E. Brickhouse, LP 5U-C D. A. Day, POB 2A-SQN S. A. Howard, OPS 5N-SQN K. Langdon, POB 2B-SQN D. B. Nida, LP 5U-C U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, DC 20555 A. A. Ray, WT 11A-K G. R. Signer, WT 6A-K M. D. Skaggs, OPS 4A-SQN B. A. Wetzel, OPS 4A-SQN K. M. Hodges (EDMS), lP 2V-C Tennessee Valley Authority , Post Office Box 2000, Soddy D a isy, Tennessee 37384-2000 February 09 , 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement

& Compliance Section 6 1 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JANUARY 2011 Enclosed is the January 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 1/29/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, " /) I ! .1-* 1 {. .. :; _.; ', -.x:::----... , ; ( , l ' Michael D. Mag;gs Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure)

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Bu i lding , Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington , DC 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

___ _ Addres_L 2000 ___________ _ _______ _ Facilitv _ _!VA -SEQUOYAH NUCLEAR PLANT _____ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) r-101 G MAJOR (SUBR 01) F-FINAL DIFFUSER DISCHARGE EFFLUENT Form Approved OMB No. 2040-{)004 ATTN: Stephanie A. Howard From *** NO DISCHARGE D ... NOTE: Read instructions before completin!l this form UNI TS QUANTITY OR LOADING QUALITY OR CONCENTRATION [ PARAMETER T><J . TEMPERATURE, WATER DEG. SAMPLE ..... _ *--* ... ""******* ******** 9.4 0 I 31 I 31 I MODELD C ENTIGRADE M EAS UR EM EN T I 04 0 00 1 0 z 0 0 + ---;;:;..;;;;......

-...,......... ..... -******** **"***"" 30.5 OEG. c. I I SEE I CK REO INSTREAM MONITORING r
REOUIREMENT I DAILy MX I PERM I T TEMPERATURE, WATER DEG. -S A MP L E uuuu .. ,.:; ...... -.---** ******** ******** I 24.3 0 4 1 0 T 31 I 31 I R C ORDR CENTIGRADE M EASUREME N T ---------* *--00010 1 0 0 PE R MIT ******....
                • ...... ******** ******** REPORT OEG. c. I I SEE I CK REO E F FLU E NT G RO S S V A L UE R EQ U IRE M E NT I I DAILy MX I PERMIT -
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"" ;;;;;:;.-** . ,.._*'""*** 3.9 0 . t 31 I 3 1 CALCTD MEASURE M E N T 04 I TEMP. OIFF. BE TW EEN S AMP. & UPST R M DE G.C I AVERAGE MAX I MUM NO. \Ff!EOUENCY I SA MPLE EX OF TYPE ANALYSIS U N I T S M I N I MUM AV E RAG E MAX I MUM P E RMI T ******** -******** **-. ............ : ............ I 5.0 . OEG. c. CALCTD RE Q U I R E MEN T I I DAI L y MX uous -S A M PLE --. ... -;. -** ----. .. ;.; .. ;;-u 7.8 T ******** 8.2*-*--0 6131 M EAS UR EMENT 12 P ERMI T *****"** . r---' REQUIREMENT [ _I ___ --1 MF SA M PLE . *******"' . 0 0 530 1 0 0 ' .... ;.;;;---******** I .... .......... I 30 I 100 EF F LU E N T GROSS VALUE I R EQ U I R E MEN T I MO AVG DAILY MX OJ L AND GREASE I S A MPLE-******-******** .. '"******* I <6 <6 ---M EA SUREM ENT 00 556 1 0 0 I ....;;...;;.... -;....... ...... -******** I' 15 20 E FFLUENT GROSS VALUE i REQUIREMENT I . MO AVG DAILY MX FL OW , IN CONDUI T O R TH R U 1 SA M PLE ******** ffi 4 g ***'"***'" I ..;;;;;;;- TREATME N T P UNT ME A SURE M ENT 03 -----* " *-*----500 5 0 1 0 0 PER M IT *"****** R E PORT I MGD ******** I --** GROSS VALUE ----DAILy MX 00016 1 w 0 E FFL UENT GROS S V A L U E PH 00 4 00 1 0 0 EFFLUENT GROSS VALUE ::-c-:-:,.-----=-=-----+ SOLIDS , T O T AL S U S PEN D ED -s.o ******** 1 9.o MINIMUM __I_M_AXIMUM ***'"**** 13 I 13 1 9 ****'**** MG/L WEEKLY I GRAB GRAB su GRAB ******** 1 I 31 0 0 1 /31 MG/L MONTHL Y l GRAB 1 9 *"'***'*'**'*' GRAB MO N THL Y l G RAi3 0 I 31/3 1 I RCORDR ......... CONTIN I RCORDR I U OUS 1 ---NAM E/TITLE P R I N CI PA L EXECUTIVE OFF ICER I C ert , f y u nde r pena lty of l aw t hat th1s do c ument and all attachments were prepared under my r1.5.f .. TELEPHONE DATE 1--'--' -----d i r ec tion or supervi s ion in accordance with a system designed to assure that qualified personnel ,.....---Michael D. Skaggs properly gather and ev aluate the informat i on submitted Based on my inauiry of the person or S equ o yah P resident p e rsons wno manage the system. or those persons directly responsible for gathering the 423 843-7001 11 02 07 In f o rmat i on , the information s ubmitted is , to the b e st of my knowledge and belief , true, accurate , Sequoyah Site Vice President c omplet e I am aware that there are s ignificant penalties fo r submiWng false information , SIGNATURE OF PRINCIPAL EXECUTIVE I *--____ -----------------____ ---l includ*ng tt"le possib i lity of fine and imprisonment for knowing VIOlations OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE -----------------------COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment:

1. CCW data 2. veliger monitoring data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2 DMR Attachment CCWData CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained.

CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 1/19/2011@ 1110 <0.10 mg/1 1/21/2011@ 1244 ND EPH Mean# of Water Mean# of Water SUB NOTES:% Sample Date ZM/m3 %Settlers Temp. ("C) Sample Date Asiatic Temp. ("C) LOCATION LOCATION Gravid Asiatic COLLECTED BY Clams/rri3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-ISV 1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV 1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB PERMITIEE NAME/ADDRESS (Include Facifitv Name/Location if Different) ___ _ AddreS£.._ _f.Q,__BOX 2000 ___________ _ _______ _ Facllitv _ _IVA_-SEQUOYAH NUCLEAR PLANT _____ _ Loca.J!O.!:L .J:!AMILTON COUNTr._ _________ _ ATTN: Stephanie A. Howard PAR A METER NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMR) From TN0026450 PERMIT NUMBER MAJOR (SUBR 01) F *FINAL DIFFUSER DISCHARGE EFFLUENT *** NO DISCHARGE D ... Form Approved OMB No. 2040-{)004 NOTE: Read instructions before completing this form. TITY OR LOADING QUALITY OR CONCENTRATION NO. EX F REQUENC Y! SAMPLE AVERAGE UNITS MAXIMUM UNITS MINIMUM MAXIMUM OF TYPE ANALYSIS _I CHLO R I N E , TOTAL R E S IDUAL 50060 1 0 0 EFFLUENT GROSS VALUE **"****** I ** ******** I 0.019 ti.038 I 19 0 I 10 I 31 GRAB I PERMIT "**""**** --.. .-.;;.;** _,.. ******** 0.1 0 . MG/L -+w EEK* CALCTD I ***-** 0 -r! 62 ******** I -L-MAX__ -cAtcTo. 1 TEMPERATU R E

  • C , RATE OF CH A NGE 82234 1 0 0

_9 ROSS VALUE PERMIT ------2"-----I DEG ----- l REQUIREMENT

_ j C/HR ________ 1 , _ _ __ SAMPLE MEASUREMENT

_l____ -- I I -ll REQUIREMENT I -----_J I ! 1-l SAMPLE MEASUREMENT PERMIT t-------1 j REOUIREMENT --+------ ---SAMPLE MEASUREMENT

*----*--*---1 PERMIT I REQUIREMENT

-'---___ ] NAME!TITLE PRINCIPAL EXECUTIVE OFFICER j' Cert*fy un der penally of law l hatlh*s dOOJmenl and all attachments we re prepared under my d lrectooo or supervision t n accordance wllh a S)l5 tem destg n ed to BSSure that Qualified persoonat -t=t ---1 :----1 --_j TELEPHONE DATE --1 I Michael D. Skaggs !p roperty ga t her and eva luate th o tnfQilllatt on subm*tted Ba s ed on my lnQu*ry or the person or 1 1 persons who manage me sy5tem, or those persons directly responsible lor gat.hering the Sequoyah Site r e sident 3 07 . . . pnlormation , the information submotled is , t o the best of mv and belrel. true. accurate. -42 C Sequoyah Stte Vtce Prestdent l and complete. 1 am aware that there are s*gnificant penames for submitt i ng false information. SIGNATURE OF PRINCIPAL EXECUTIVE ___ includrng lhe possrbrhty of fine and rmprr so nment for knowong vrolalrons OFFICER OR AUTHORIZED AGENT AREA DAY TYPED OR PRINTED CODE . --*----*----COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The only B/CTP chemical injection this period was Towerbrom 960. EPA Form 3320*1 (REV 3/99) Previous editions may be used Page 2 of 2 PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) ___ _ Addres_L 2000 ___________ _ _______ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) !--T-N0026450 -1 L PERMIT NUMBER From _. MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040.{)004 BIOMONITORING FOR OUTFALL 101 EFFLUENT *** NO DISCHARGE D ... NOTE: Read instructions before comt>letinQ this form PARAMETER / . -QUANTITY OR LOADING -QUALITY OR CONCENTRATION N O. FREQUEN C Y S A MPLE IC25 STATRE 7DAY CHR C ERIODAPHNIA EX O F TYPE AVERAGE MAXIMUM U N ITS M I NIMU M A VERAGE MAX IM UM U NI TS ANAlYS IS SAMP L E MEASUREM E *uuu* ******** .. Monitoring

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_ ,__ --+------1-----l I TRP3B 1 0 0 NT GROSS VALUE PERM I T R EQ U I REMEN I -**"***** ..... 45.2 **-** **-**** PERCENT S EE CO MP O S I T MIN I M U M 1 PER MIT I C2 5 STATRE 7DAY CHR PI M EP HA LE S TR P6C 1 0 0 [E FFLUENT GROSS VALUE P E RMIT R E QUIREMEN ----+-----1 NT 1 :::-:-:-:--+/-*--= * .::. = = PE: Nl ___ S_E_E ___ 1 C O M POS . -MlMINUM I . . I P E R MIT *-j S AM PLE MEASUR E ME P E RMIT REQUIREME I I ! i -----*---------*--* -------*-**------+-- --+--c------*-SAMP L E PERMIT REQUIRE M EN J ______ I I I r I ' ----*-,----- ---+----1---------+----- ---l--------+----+--+---+--

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-I -------REQUIREMEN T 1 SAMPLE ---..,___ -I I PER M IT ----REOUIREME SAM P L E MEASUREM ----------+---- PERM I T REOUIREMEI -_ J i_ _ L _L ___ J NAMEITIT LE PRINCIPA L EXECUTIVE O FF I CER I C ert o l y underoenally of l aw tha t thos documem ana all altachme ntli we r e p r e p a r ed under my G LJ I --------l dorect1on or supe,....,s*on 1n ac c ordance Wlth a system designed to assure lhal qualifi ed personnel l ) .-M i chael D. Skaggs properly gal her and the mlormat1on s u b m tl ted Based on my Inq u i ry of the person or 1 / \ persons who me n agelhe s)'!lem. or those per sons dlrOOil y r espon5ible l o r gatherong the Sequoyah S i te "-i e President . . . onformato on , lhe m l ormallon s ubm11ted ts . lo th e besl of my a n d behel, true, accurate, __ ........ Sequoyah S 1 te V1ce President and com plete 1 am aware 1 ha1lhere are s ogntfooant penalties f o r submottmg fa l se onformatoon , SIGNATURE OF PRINCIPAL EXECUTIVE __ _ _jin c lud i ng I he oosS<bi l o l y o r fine and lmprosonme n t for knowmg vi olations. I OFFICER OR AUTHORIZED AGENT TYPED OR PRINITED _ __ _____ . I. COMMENT$ AND OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in January 2011. EPA Form 3320-1 (REV 3/99) Previous editions may be used TELEPHONE I DATE 423 843-7001 11 1 02 07 AREA T NUMBER YEAR MO I DAY COD E Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L __E.Q.,_BOX 2000 ___________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 _______ _ LOW VOL. WASTE TREATMENT POND EFFLUENT ATTN: Stephanie A. Howard From *** NO DISCHARGE D ... NOTE: Read instructions before completin!l this form. PARAMETER / QUANTITY OR LOADING QUA U TY OR C ON CE N T RA TI ON PH AV E RAG E ,, E NT MIN I MUM I AVERAGE ----6.8 *'******* MAXIMUM -7.8 I UNITS I I ***................. .. 00400 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00 530 1 0 0 EFFL UENT GROSS VALUE PERM IT REQUIREMENT SAMP LE MEA S UREM E NT ........... -t--------1 124 P E RM IT 380 RE Q U I R E MENT I MOAV G ********* .. --* 298 2 6 1250 LBS/DY DAI L Y MX OIL AND GREASE 66 I 26 r-------S AMP LE <60 --M E A S U RE M E N T 00556 1 0 0 I' PERMI T -1 90 E FFLUENT GROSS VALUE _ M Q AVG_ 250 i LBS/DY FL OW, IN CONDUIT OR THRU S AMP LE 1 11 Q T RE A TMENT MEA S UR E M E N T . 50050 1 0 0 I -PERMIT E FFLUENT GROSS VALUE REQUIREMEN T SAMPL E REPORT .P AIL Y IYIX -+---1.355 03 --I 6.0 ****'**** MINIMUM I --***""**"' 13 9.0 MAXIMUM 30 ................. I 30 MOAVG 100 DAILY MX ....,.......... I <6 ---******** 15 <6 20 MOAVG --r* ------ ........... ** _DAILY MX ***1r-1r-1rlr* ----I UN I TS I 12 I su I 1 9 I MG/L 19 I MG/L .. .. N O. I F FU:O UE N C Y I S AMP L E EX O F TY P E 0 0 ANALY SIS 15 I 3 1 GRAB G R A B I GRAB 4/3 1 GRAB ----WEEKLY j GRAB o r 31 ;31 ---T OTALZ 1--I I -I I f -1 I I I I R E QUIR E MENT . __ j ----1 F I I ! I I t-l __ I I i -j __ ] NAM E/T ITL E PR I N CI PA L EX ECUTI V E O FFICE R J t Cer1ofy under penalty of taw lhalthls doc;ul'l'enl and all attachmenls were prepa r ed under my ------'diredlon ot supervision In accordance Wi t h a system designed 10 assure that quahfied personnel TELEPHONE DATE M ic hael D. Skagg s properly gather and evaluate the lnformahon submilled Based on my In quiry of the person or persons wf)o manage the syslem, orlhose persons directly responsible fDf galher1ng the r Sequoyah Slie I , . . onformaUon , lhe sut>motled os. to tne bes t of my knowledge and belief. true , a<:cumle , j L...:::': . Sequoy a h S 1 te Vic e P r estd e nt and complele 1 am awa r e thai there are slgmficant penalties for submllting false onformalion , SIGNATURE OF PRINCIPAL EXECUTIVE

!'ncJudrng lhe p0$Siblllly of fine and lmpnsonroent lot knowrng Vlolal l ons OFFICER OR AUTHORIZED AGENT TYPED OR PR I NT E D 843-7001 423 11 I 02 I 07 NUMBER YEAR I MO I DAY AREA I CODE -"-----l COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all atrachments here) EPA Form 3320*1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different)

___ _ Addres.§__ _f.Q,_BOX 2000 ___________ _ _______ _ _____ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) -TN0026450 I From MAJOR (SUBR 01) F-FINAL METAL CLEANING WASTE POND EFFLUENT ... NO DISCHARGE I XX I ... Form Approved OMB No. 2040-0004 NOTE: Read in s truct i ons be f ore complet i nQ this form. PARAMET<R [?><[. QUANTITY OR LOADING QUALITY OR CONCENTRATION N O. I F REOUENCY I SAMPLE EX OF TYPE A VE RA GE M I NIMUM I AV E RAG E MAX I MUM I U N ITS ANALYSIS 12 PH SAMPLE ..,.****** MEASUREMENT

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9.0 su --r-MMIMUU , 0 4 00 1 0 0 PERMIT -EFFLUE N T GR O SS VALUE R EQUIREMENT S O LI D S. TOTAL S U S PENDED r SAMPL E I I ---6.0 I ""******'* M j_NIMUM -*----******** [' DAI L y I GRAB I I oAt L I MEASUREMENT I 1 00530 1 0 0 PER M IT GROSS VALUE i R EQU I RE M ENT l OlL AND GREASE j-S A MPLE -i 00556 1 0 0 EFFLUENT G RO SS V A LUE ---PH OSP H OR U S , T O TAL (A S P) I MEASUREMENT PERMIT REQUIREMENT ' .... ----******** 1 SAMPLE ******** I MEASUREMENT -........... --+-_j 1 9 I ............... ............ ******** ***'***** 30 MG/L ***"'*"**** DAILY MY --+-----* .!!,!.*** ---l---t-----J-----!=-= -******** 19 ******** I ******** ' 15 I MG/L I I DAILy I GRAB I DAILY MX I --f---********-*t--.,;;,o;.,;;.. -;;-**t------ +---+ ----1 9 I I 1 00665 1 0 0 EFFLUENT GROSS VALUE !COPPE R , T O TAL (AS CU) -f.--**** I .. _.. I 1.0 I ... I I DAILY '!COMPOS . , DA I LY:l . . 1 I *-.,;;;;;;;

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1 ---* -------*----*r-.. ------.. ------------------4-------1 PERMIT I ******** I ******** I REQUIREMENT i . I 19 MG/L DAI L Y I COMPOS 01042 1 0 0 GROSS VALUE fR oN , TOTAL (AS FE) 01 045 0 0 .... ...--..... ,_ -..... _ .,_.... I 1.0 I r _. ... m ****-..,...,.-******** ******"" DAILY _I\IIX M EASURE M ENT ** I -;;;; *** ;--.... 1---;;;..... I -;;;:;;; *** 19 i 1 DAILY I COMPO S MG/L 1.0 REQUIRE M EN T I l 1---E FFLUENT GROSS VALUE -------DAILY MX +----FL OW , IN CONDUI T OR TH RU TREATMENT PU N T ........... 03 *'*******

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  • ******* 50050 1 0 0 EFFLUENT G ROS S V A LUE ,-PER-MIT l __ R_E_P_O-RT I MGD _j : -I VlP_AV G DAILy MX L__ ---__ j NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1 1 Cert1ly under penal t y o. I taw t ha t t his document and al l attachments were p r epa r ed un d e r my I * -dtrect10n or s u perviS I O n I n accorda n ce Wlth a sys t em designed to as sure t h a t Quali fi ed p erson n el Michael D. Skaggs p ro perly g at her and the mf cmJa l lon su b mllwd Based o n my of l he p erson or persons who manage t t>e syslem. o< th ose pe r sons d irectly responsible f or g at herin g iJl e . . . infO<milhon. t he 1 n forma t io n su b mm e<t ts
  • to t/1e bes t o f my k nowledg e and belief t ru e, accurn t e , 1 Sequoyah S1te Vrce President and com pl e t e 1 a m aware l h a llller e are sigmfi can t penalties I a< s ub mi t llng false i n f ormation. S eq uo y ah Sit e :vicelEresi d e nt SIGNATURE OF PRINCIPAL EXECUTIVE HORIZED AGENT I I I TE L EPH ON E 423 843-7001 OAIL Y I CALCTO L___ --'--__ ] D A T E 1 1 02 I 07 M O I DAY t**--____ ___ ____ 1nclud1ng the p oss1 b llity of fine en d ompnsoome n llor knOWing v.ola t1 ons I OFFICER OR AUl TYPED OR PRINTED CODE , . ----------------------" ..::.::;.:::::__;__ ____ _J_ _ _j_ __ ._____, COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199)

,;ay be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _

_______ _ Facility_ ..JYA-SEQUOYAH NUCLEAR PLANT _____ _ Locatio.!l._ __!iAMILTON COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 From MAJOR (SUBR 01) F-FINAL RECYCLED COOLING WATER EFFLUENT ... NO DISCHARGE I XX I *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO. !FREQUENCY SAMPLE TEMPERATURE, WATER DEG. CENTIGRADE ENT AVERAGE MAXIMUM I UNITS I ******** MINIMUM AVERAGE . .................

          • '*** EX OF TYPE MAXIMUM UNITS ANALYSIS 04 -DEGC 1--I DAILy I' GRAB-4 . 38.3 000 10 z 0 0 I NSTREAM MONITORING I PERMIT -1, --* ..-..: * .....---I REQUIREMENT , .. -.-.-.... -.. 1----1-----t------t--'D=A'-" I=L-'-Y_,_, M=X I PH 004 00 1 0 0 E FFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 0 0 PERMrT REQUIREMENT EASUREMENT

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T ....... ,.;.,. ------i--------1 PERMIT **<rlrlr*"* "'"****** EFF LUENT GROSS VALUE OIL AND GREASE i REQUIREMENT

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--f---******** 00556 0 0 EFFLUE NT GROSS VALUE CHLORINE , TOTAL RESIDUAL 50060 0 0 E FFLUENT GROSS VALUE PERMIT I ., .............

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REPORT MOAVG SAMPLE ********

  • r-= _ r PERMIT --REPORT DAILYMX ********

1 _ ----SAMPLE MEASUREMENT L -=-r ------pER M IT -J ...... " 03 MGO ... ..... 6.0 "*'*'*'***** **-******** ******** ******** i ****** .. 19 30 MG/L DAILY MX=----1!-----.-19 --MG/L 1 DAILY I COMPOS -* ---+ ------1 DAILY GRAB ------................... ******** ......... . ...... . MX I I +------1--*** I *=* F DAILY CALCTD -I 0.10 I ******** **+***** ******** i *"'****** I tDAILYMX I --*-* I ----_! DATE NAMEfT ITLE PRINCIPAL EXECUTIVE OFFICER j l Certify under penalty of law lhat thiS document and all attachments were prepared under my -----* ----dlrecllon or supeMsion In accordance with a system designed to assure that qua hfied personnel TELEPHONE Michael D. Skaggs gathl!f and evaluate the lniO!'ITIIItton subr111tted Based on my rnou*ry of the person or persons v.'ho manage the system. ot those perSons dlrectly responsible for gathenng the . . . 1nforma uon. the Information subm*tted I s. to the best cl my knowledge and belief. true. accurate. Sequoyah Stte Vtce Presrdent l and complete 1 am aware that there are s*gmficant penalties lor submiWng false inlormabon , I SIGNATURE OF PRINCIPAL EXECUTIVE -;*nclud*ng the possibility or fine and *mpnsontnel1t for knowtng Vloiahons OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED 423 843-7001 AREA I NUMBER CODE 11 I 02* I 07 YEAR\ MO I DAY ---------------' COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITIEE NAMEJADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-{)004 Addres_L _l'_.Q,_BOX 2000 ___________ _ ATIN: Stephanie A. Howard PARAMETER TN 0 026450 From QUANTITY OR LOADING MAXJMUM UNITS MINIMUM 110 T RECYCLED COOLING WATER EFFLUENT NO DISCHARGE I XX I ... NOTE: Read instructions before comoletinQ this form QUALITY OR CONCENTRATION NO. I F R E O UE N C Y I S AM PLE EX O F TY P E AVERAGE MAXIMUM UNITS ANALY SI S AVERAGE I C25 STATRE 7DAY CHR CE R I O DAPH NI A SAMPLE MEASUREMENT I *--******** I I -lr---.-.....,---.. -.. ............ 2 3 TRP3B 1 0 0 I PERMIT ............ EFFLUENT GROSS VALUE I RE Q U I REMENT I *******'* -* 45.2 MINIMUM ********I ............ I PERCENT M SEMI COMPOS I *****-I --** I 2 3 ' --IC 2 5 S T ATR E 7 DA Y C HR ... PIME P HALE S

  • TRP6C 1 0 0 EFFLUENT GROSS VALUE I ___ _____, 1" .............

I PERMIT ----r-......,.,;;-T' - 1 REQUIR E MENT I 45.2 MINIMUM 1 ******** , PER CE N T is EMI I coMPos 1 -j I ******** SAMP LE MEASUR E M E N T I P E RMIT --t-------f-----i REQU I REMENT 1 . : I 1---------SAMP L E l PERMIT I ____ ---SAMPLE M E ASUREMENT I r P E RMIT I REQUIR E MENT I j---I J I SAMPLE *-j I PERM I T ' I REQUIREM E N T I ----+---t----------t r----------+ SAMPL E I MEA SU REMENT _____ __!__ -PERMI T t---l __ j I l REQUIREMENT __ NAMEJTITLE PRINCIPAL EXECUTIVE OFFICER J l Cerlofy under penally of l aw that lhos dowment all aUachmams were pr epared my or supeMSoon on accordance with a sys l em c;!esog n ed tP assure lha t Q uatofied personnel Michael D. Skaggs properly galher and evalual1! lhe Information submtted Based on my Inquiry of lhe persQ!l or !persons who manage lhc system. or those persons dlreclly respooslble f or ga l henng t he . . . jt nformalion , the ln f ormatoon subl)ll t ted Is. to lhe besl of my knowtedge an d belief. lrue , accura l e. Sequoyah S1te Vice Pres1dent and complele , 1 am aware that t h e<e are slgnlflcan l pene llo es f or su b mi tt ong fal se onrorma t ion. __ !oncludlng lhe possibility of r.ne and lmpnsonmen t f or vlola li ons TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Sequoyah S i te-<<L!Ce Pres i dent SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT I -I I I_ I ___ TELEPHONE DATE 843-7001 11 . 02 I 07 NUMBER YEAR MO I DAY Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ Addres_L _.E.Q,_BOX 2000 ___________ _ ATTN: Stephanie A. Howard ----NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 I 116 G PERMIT ___, DISCHARGE NUMBER I f¥1 0N!IqfillJ.G -,E E_RIPJ? . I ... I From MAJOR (SUBR 01} F-FINAL BACKWASH EFFLUENT *** NO DISCHARGE CJ *** Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completi11!1 this form PARAMETER QUANTITY OR LOADING AVERAGE -MAXiM UM QUALITY OR CONCENTRATION N O. l"Kt OUENCY I SAMPLE U N ITS MINIMUM AVERAGE MAXIMUM U NIT S EX OF TYPE ANA LYS IS 1 I 31 VISUAL ..,_....,. ............. '* ................ ............... 0 0 9A DEBRIS, FLOATING (SEVERITY) ----1 01345 1 0 0 PERMIT ____ ******** --I R E POR T I P A SS=O I SEE VISUAL ******** -*'*'******

                • EFFLUENT GROSS VALUE 1 REQUIREMENT

\ OIL AND GREASE VISUAL SAMPLE 0 .....................

  • 94 -PE RMI T + -.........

t-REP OR T I YE S=1 MO TQ T A l I FA.IL=1 PERMIT I ,_ _ I "****** J .. l o

        • .............

84066 0 0 EFFLUENT GROSS VALUE

  • M_O _T O TAl N O=O SAMPLE PERMIT REQUIREMENT J I I 1--1--I I I I SAMPLE PERMIT REQUIREMENT

--_j SAMPLE PER M IT REQUIREMENT SAMPLE : _I __, I I r* I rt __ . ___ !_,-I I \= j I I I I --I I i i ---* : ' --*-: I I ---PERMIT

  • I I 1 __________

--. l Jl . I PERMIT I I I I ...J. -_ _ --' 1 1 _ _ _ __ _ NAME/TITLE PRINCIPAL EXECUTlVE OFFICER I t Cert o fy under penalty of l aw lhat lhts document and ali auacnmenl$ were prepared undeo-my -------------'d*rectlon or supervoslon In accordance woth a system desogned to assure that qualified personn el Michael D. Skaggs prcpeny gather and evaluate the tnfonnatooo submlt1ed Based oo my mqu*ry of the person or persons wl1o manage the system. or those persons dlreclly responsible for gothoring the . . . .n forma bon. the Information subml lled IS to the besl of my knowledge and belief. true. accurat e, Sequoyah Stte Vtce Prestdent .and complete. 1 am aware tnat there are Significan t penalbes for submo!tmg false i nformation. ____ ___joncludmg the POSSib ility of line and i mprisonment for knOWing v!Oiabons TYPED OR PRINTED --------------COMMENTS AND OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used DATE 843-7001 I 11 I 02 07 NUMBER I YEAR I MO I DAY Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ _!YA

  • PLANT ___ _

_______ _ Facilitv _ _IVA -SEQUOYAH NUCLEAR PLANT _____ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) F TN0026450 PERMIT__N!)MBER MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard From . _ *** NO DISCHARGE D ... PARAMETER QUANTlTY OR LOADING I I AVERAGE UNITS I S A MPLE MEASUREMENT ---1----.............. DEBRIS, FLOATING (SEVERITY) -__ j PERMIT I ........ . REQUIREMENT 0 1345 0 0 MINIMUM *******ill NOTE: Read instructions before comoletirlll this form. NO. I FREQUENCY I SAMPLE-EX OF TYPE ANALYSIS QUALITY OR CONCENTRATION AV ERAGE M A XI M UM I UNI T S --0 I 9A 1 I 31 VISUAL ******** 0 REPORT I PASS=O FAIL=1 VISUAL I SEE EFFLUENT GROSS VALUE OIL AND GREASE VISUAL SAMPLE-I MEASUREMENT

I I 0 I I _!YIOTOTAt

                • .. J P E R MIT i I 0--1 /31 V I SU A L 1 84 066 1 0 0 E FFLUENT GROSS VALUE ------PERMIT REQUIREMENT I r-PERMIT -I ....... -RT MQTOTAL_ !+I SEE I V I SU AL I P ER MI T --t-----*

I REQUIREMENT I 1-----------1 ---* ---r-----+-------SAMPLE MEASUREMENT 1 I . PERMIT--j-----j I REQUIREME N T --. -SAMPLE MEASUREMENT I I IH-PERMIT REQUIREMENT I I I . I I --------1 L__ PERM I T REQUIREMENT

1-*--NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I I Certify under penalty of law lhat this document and all attachments were prepared under my or supervision in accordance with a system designed to assure that qualified person ne l Michael D. Skaggs prop erly gather and evaluate the information submitted.

Based on my inquiry of the person or pers ons who manage the system, or those persons directly responsible for gathering the I nfor mation, the information submitted is , to the best of my knowledge and belief, true, accurate. Sequoyah Site Vice President a n d complete. I am aware that there are significant penalties for submitting false information. TYPED OR PRINTED I the possibility of fine and imprisonment for knowing violations COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320..1 (REV 3/99) Previous editions may be used _j Sequoyah SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT I I_.L_L_J TELEPHONE DATE 423 I 843-7001 11 02 07 AREA _j NUMBER YEAR MO DAY CODE Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facililv Name/Location if Different) ___ _

_______ _ Locatio.!l._ _!:!AMILTO.!i.COUNTY __________ _ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) L. I -. _ ....... **-***--** I 118 G DAY From 31 I PARAMETER QUANTITY OR LOADING MAXIMUM OXYGEN , DISSOLVED (DO) 00 3 00 0 0 E FFLUENT GROSS VALUE SAMP L E MEASUREMENT ---P E RM I T REQUIR E MENT ........... ._ .. ******** ******** --******-;;; --r ********' ._. 2.0 I ******** I DAILY MN I MAJOR (SUBR 01) F-FINAL WASTEWATER & STORM WATER EFFLUENT ... NO DISCHARGE I XX I ... Form Approved OMB No. 2040-0004 NOTE: Read instructions before completinq this form. I UNITS ******** I 19 ******** I MG/L Y-,,RA B I WEEK ---SOLIDS, TOTAL SUSPENDED I 1 00530 1 0 0 SAMP LE MEASUREMENT PERMIT ----******** 100 I MG/L *"*******

                • -I ***.............

-******** .. -l_.L __ . _:J I TWICE/ GRAB I ..... ******** ******** ............. E FFLUENT GROSS VALUE l souDS. SETTLEABLE

REQUIREMENT SAMP LE MEASUREM E NT .............

I--****--** ... ****- 25 r-f-WEEK . ' l ******** I .... ;;;;;---: .... ******** I ........ I MX MUL ,- I GRAB 00545 1 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 0 EFFLUENT GROSS VALUE -**-t 03 _:_ri*....

    • +--[=J PERMIT t-REPORT REPORT MGD -.,* ******** ! ********
  • ONC E/ ESTIMA REQUIREM E N T I MO AVG DAILY MX I I BATCH I ! .

--I -------] ______ -I______ J ---PERMIT* I I L I I I -I REQUIREMENT I : --i ; ---SAMPLE t MEASUREMENT ---*------*-PERMIT ----1 I SAMPL E l MEASUR E MENT PERMI T -1 l L I REQUIR E MENT I I I L._ ___j_ ___ i L I _j NAM EfTITLE PRIN C IPA L EXECUT I V E OFF I CER I I Cer111y un der penalty of law tnat 1n 1 s document and all a t tachmen ts wer13 P<eoa1ed u nder my ----'d ir ecti o n or s u pe<v 1t;1 on 1n acco r dance With a system des1gne d to assu r e that personnel M ic hael D. Skaggs prope r1 y ga l her and eva lu ale lhe 1 nforma u on su bm*tt ed Based on my 1 nqu1ry of the person or pe rsons v.Nl manage lhc system , or those persons directly respons i ble f or ga l henng lhe tn format*o n , the I nformation s ubm i tted I s , to lhe besl or my know l edge a!ld be li ef , true , accurate. ' £ 1 _j a!ld compl ete. I am awa r e tha t t here are s l g m ftcant penalties for subtn11ting fals e I nformation. -* * * * .. -.. *-*-.. -----.... *-" the llOSS*b l hty of fine and l mpnsonmentfQ( knowing v.OiaUoos l Sequoyah S i t e V i ce Pres i dent TYP E D OR PRINTED ----COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used TELEPHONE DATE 423 843-7001 11 I 02 I 07 YEAR MO I DAY Page 1 of 1 REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-January 2011 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 02/07/2011 CONCURRENCES Name R c Signature -Comment Date v N B.M. Love X S. A. Howard X D.A. Day X M.D. Skaggs X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. S58 110308 800-NPDES CORRESPONDENCE March 8 , 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor , L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-D I SCHARGE MONITOR I NG REPORT FOR FEBRUARY 2011 Enclosed is the February 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond In l et of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 2/26/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information , please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system , or those persons directly responsible for gathering the information , the information submitted is , to the best of my knowledge and belief, true , accurate , and complete. I am aware that there are significant penalties for submitting false information , including the possibility of fine and imprisonment for knowing violations. Sincerely, Site V i ce President Sequoyah Nuclear Plant Enclosure cc (Enclosure)

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building , Suite 550 540 McCallie Avenue Chattanooga , Tennessee 37402-20 1 3 cc: B. E. Brickhouse , LP 5U-C G. M. Cook , OPS 4A-SQN D. A Day , POB 2A-SQN S. A Howard , OPS SN-SQN K. Langdon , POB 28-SQN U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington , DC 20555 D. B. Nida , LP SU-C A A Ray , WT 11A-K G. R. Signer , WT 6A-K M.D. Skaggs , OPS 4A-SQN K. M. Hodges (EDMS), LP 2V-C Tennessee Valley Authority, Po s t Offi ce Bo x 2000 , S o ddy Dai s y, T e nne ss e e 3 7384-2000 March 8, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement

& Compliance Sect i on 6 1 h F l oor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MON I TOR I NG REPORT FOR FEBRUARY 2011 Enclosed is the February 2011 Discharge Monitoring Report for Sequoyah Nuclear P l ant. Sample co l lection continues at the Diffuser Pond Inlet of the Yard Drainage Pond eff l uent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 2/26/2011 have all yielded resu l ts below detection limits for oil and grease. If you have any questions or need additional information , please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system , or those persons directly responsible for gathering the information , the information submitted is , to the best of my knowledge and belief, true , accurate , and complete. I am aware that there are significant penalties for submitting false information , including the possibility of fine and imprisonment for knowing violations. Sincerely , Site Vice President Sequoyah Nuc l ear Plant Enclosure cc (Enclosure): Chattanooga Env i ronmental F i eld Office Division of Water Pollution Control State Office Bui l ding , Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Wash i ngton , DC 20555 PERMITTEE NAME/ADDRESS (Include Facility Na me/l ocation if Differen O NATIONAL POUUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (D M R) MAJOR (SUB R 01) _p....Q.JlO.X.ZOO!L __________ _ ______ _ ____ _ ATTN: stephanie A. Howard PARAMETER X OUANrrrY OR LOADING OUAUTY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM TEMPERATURE , WATER DEG. SAMPlE ..........

                • .............

............. 12.8 -CENTIGRADE MEASUREMENT 00010 z 0 0 PERMIT *****"***

                • -******** ******** 30.5 INSTREAM MONITORING REQUIREMENT DAILYMX TEMPERATURE, WATER DEG. SAMPlE ...........
  • '******'*
                • **'*'***** 25.3 -CENTIGRADE MEASUREMENT 00010 1 0 0 PERMIT ******** ******** ...... ******** ............

REPORT EFFLUENT GROSS VALUE REQUIREMENT DAILY MX TEMP. DIFF. BETWEEN SAMP. & SAMPlE ******"** "******* *"*"'****** ........... ... 3.5 -UP STRM DEG.C MEASUREMENT 00016 1 w 0 PERMIT ******** ******** -** *"***** ******** 5.0 EFFLUENT GROSS VALUE REQUIREMENT DAILYMX PH SAMPlE ............

        • "'*-8.4 ******** 8.6 MEASUREMENT

-00400 1 0 0 PERMIT ******** ******** .... 6.0 .........

9.0 EFFLUENT

GROSS VALUE REQUIREMENT MINIMUM MAXIMUM SOUDS , TOTAL SUSPENDED SAMPLE ...........

              • "*
          • '***

7 7 ... MEA S UREMENT 005 30 1 0 0 PERMIT *"******* --*'******* 30 100 EFFLUENT GROSS VALUE REQUIREMENT MOAVG DAILY MX OIL AND GREASE SAMPLE *******'*

              • "*
                • <6 <6 -MEASUREMENT 00556 1 0 0 PERMIT "'*"'*****
  • "*****"**
        • ******** 15 20 EFFLUENT GROSS VALUE REQUIREMENT MOAVG DAILY MX FLOW, IN CONDUIT OR THRU SAMPlE _ .... 1641 -.............
                • iTREA TMENT PLANT MEASUREMENT 03 50050 1 0 0 PERMIT ........... REPORT MGD *******'*
                • *******'* EFFLUENT GROSS VALUE REQUIREMENT DAILY MX NAME/TITL E PRINCIPAL EXECUTIVE OFFICER I Certify under penalty or law lhallhis doaJmenl and all a ttaclwnents were prepared under my uio eclion << supervision in aa:orclance wilh a system designed to assu-e Ulal qualifi ed per some! Michael D. Skaggs properly gather and evaluate lhe onlonnallon sullmrtted.

Based on my inquwy or lhe per$011 or persons who manage lhe system.. << those persons diredJy responsible lor ga!h&ing lhe Sequoyah Site Vice Pres i dent information. lhe lnlormation submotled is

  • to lhe best of my knaMedge and bebel , true, accurate, jand complet e. I am aware Ulallhere are sognoficam penal ties I<< submitting fa l se inrormallon.

SIGNATURE OF PRINC I PAL EXECUTIVE ondudong lhe possib<lity o r r.ne and omprisonment I<< knowing violations OFFICER OR Al.ITHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOlATIONS (Reference all Jttachments h e r e No c losed mode operat i on. The fo llowi ng i nformation is include<i in an attachment:

1. CCW da ta 2. Veliger mon itori ng data EPA Form 332().1 (REV 3199) Previous editions may be used UNITS 04 DEG.C. 04 DEG.C . 04 OEG.C. 12 su 19 MGn.. 19 MGn.. .. -Form ApProved. OMB No. 2040-0004 NO. FREQUENCY SAMPLE EX OF TYPE ANAlYS IS 0 28 I 28 MODELD SEE CKREQ PERMIT 0 28 1 28 RCORDR SEE CKR E Q PERMIT 0 28 I 28 CALC TO CONTIN CALCTD uous 0 4/28 GRAB WEEKLY GRAB 0 1/28 GRAB MONTHL'I' GRAB 0 1 I 28 GRAB MONTHLY GRAB 0 28 I 28 RCORDR CONTIN RCORDR uous TELEPHONE DATE 423 843-7001 11 03 05 I NUMBER YEAR MO DAY Page 1 of 2 DMR Attachment CCWData CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained.

CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 2/9/2011 @ 1120 <0.10 mg/1 2/11/2011@ 1214 ND EPH Mean# of Water Mean# of Water SUB NOTES:% Sample Date ZM/m3 %Settlers Temp.fC) Sample Date Asiatic Temp.fC) LOCATION LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-ISV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB 02/08/2011 0 0 9 02/08/2011 0 9 1-25-545 MJW 02/15/2011 0 0 23 02/15/2011 0 23 1-25-545 MLW PERMITIEE NAM E/ADDRE SS (Includ e Fa cility Namell..ocation rf Differen t) NAnONAL POLLUT ANT DISCHARGE EUMI NA nO N SYSTEM (N P O ES} DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) F oon APProved. OMB No. 2040-0004 __________ _ ______ _ ____ _ _________ _ EFFLUENT ATIN: stephanie A. Howard *-NO DISCHARGE D ... NOT£: Read inslructioos before oomple li ll!l th is fo rm PARAMETER X QUANTITY OR LO ADI NG QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TIPE AVERAGE MAXIMUM UN I TS MINIMUM AVERAGE MAXIMUM UNITS ANALYS I S CHLORINE , TOTAL RESIDUAL SAMPLE ******** . ..__ ******** 0.026 0.042 0 11/28 GRAB -19 MEASUREMENT 50060 1 0 0 PERMIT ......... *** ******** .... ******** 0.10 0.1 0 MG/l WEEK-CALCTD EFFLUENT GROSS VALUE REQUIREMENT MOAVG INSTMAX DAYS TEMPERATURE-C , RATE OF SAMPLE ******** 0 .........

    • ....... 0 28/28 CALCTD 62 .. CHANGE M EASUREMENT 82234 1 0 0 PERMIT ******"**

2 DEG *'*******

    • '******
  • '****** -CONT IN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX C/HR uous BORON , TOTAL SAMPLE .........

.........

                • <0.20 ............... 0 4/29

-19 MEA S UREMEN T I 010 2 2 1 0 0 PERMIT ............. -**** -******'** REPORT ******** MG/l EFFLUENT GROSS VALUE REQUIREMENT s/u SAMPLE QTF.L'( MEAS U REMEN T PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREM E NT SAMPLE MEAS U REMENT PERMIT REQU I REMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTNE OFFICER I CertJiy o..nder penany of law that thos doaJmenl Md all allac:lwnents were ln!er my TELEPHONE DATE direction or supervision in ac:axdaoce with a sys t em designed to assure that qua rifted pe<sonnel M i chae l D. Skaggs property gather and evaluate lh e information submitted Based en my inquiry oflhe pe<son or persons who manage the system, or those persons cliredly responsible for galhenng the onfonnatJon. lhe informatJan submitted IS

  • to lhe bes t of my knawtedge and belief. true , aa:onte , 423 843-7001 11 03 05 Sequoyah S i te Vice Pres i dent and canplele I am aware that then! are sq,ifcanl penalbes for SLOmll.ng false informa6on, SIGNATURE OF PRINCIPAL EXECUTIVE I oncluding the possibility of fme and mprisonment for knowing violations..

OFFICER OR AUTHORIZED AGEN T NUMBER YEAR MO TIPED OR PRINTED DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS fReferenc e all attachments her e The fo ll owing 8/CTP inj ections o cc u rred this period: B i odetergent 73551 (max. calc. cone. was 0.02mg/L-Iim i t 2.0mg/L) EPA Fonn 3320-1 (REV 3/99) P reviou s editions ma y be u sed Page 2 of 2 PERMITTEE NAM E/ADDRESS (Inclu de F ad/ltv Na me/L ocat io n if Differe n t) NATIONAL P O U.UTANT D IS CHARG E EUMI NA TlO N SYSTEM (NP D ES} DISCHARGE MONITORING REPORT (D MR J MAJOR (SUBR 01) Form APProved. OMB No 2 040-0004 _p...Q .. !!.PX2J.l91L __________ _ olscHARGE 1 I FoR ouTFALL 101 ____ _ EFFLUENT ATIN: stephanie A. Howard MD 02 ""* NO DISCHARGE o*-NOTE: Read i nstructions befo r e th i s foon. PARAMETER X QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FR£QUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ******** ******** Monitoring ............

                • -23 CERIODAPHNIA MEASUREMEN T Not Requ i red ITRP38 1 0 0 PERMIT "******* ******** -45.2 ***""*"" """**""" PERCENT SEE COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM PERMIT IC25 STATRE 7DAY CHR SAMP L E ******** *****'***

Monitoring

                • --23 PIMEPHALES MEASUREMENT Not Required !RP6C 1 0 0 PERMIT '*******'*
                • -45.2 ******** ******** PERCENT SEE COMPOS EFFLUENT GROSS VALUE REQUIREMENT MIMINUM PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMP L E MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQU I REMENT SAMPLE MEASUR E MENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TinE PRINCIPAL EXECUTIVE OFFICER I Certify Ulder penalty of law lha llhls docl.wneR and a l a l1ac:hmenls were prepared under my TELEPHONE D ATE diredJoo or supe<Vision in accordance

..mh a syste m t o assLQ lhat qua lified pers onnel M i chae l D. Skaggs property g a ther and ev a lu a 1e the infonnalion submlll ed. Based on my inquiry or the person or persons who manage the system, or those persons <Wectly cesponslble for ga1hering the tnformallon, the information Slbnilled is. to the best or my l<nowledge and belief, true. ac:cua t e, Sequoyah S i te Vice and complete I am aware lhat there are signifocant penalbes lor Stbmitttng fa l se tnlormabon., includtng the poss;bility or f1fle and omprisonment l or t<now;ng viol a tions TYPED OR PR I NTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS f Reference all attachments here Toxicity was no t sampled in February 201 1. EPA Fonn 3320-1 (REV 3/99) P reviou s ed iti ons m a y be us ed SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR ALrrHORIZED AGENT 423 843-7001 11 03 05 I NUMBER YEAR MD DAY Page 1 of 1 P ERMITT EE NAM E/ADDRES S (Includ e Faolity N ame/location ff D iff eren t) NATIONAL POU.UT ANT DISCHARG E E U M I NA TIO N SYSTEM (NPDE S) DISCHARGE MONITORING REPORT (DMR} MAJOR (SUBR01) Fonn Approyed. OMB N o. 204 0-0004 M!tr.lill_P..Q .Ji0X2Jl90 ___________ _ 64 ,;;..u.;..M 5;;..s 0;;..eR----111 olscHARoe 1 I TREATMENT PoNo ___ _ _________ _ EFFLUENT -* NO DISCHARGE o-ATTN: stephanie A. Howard NOTE: Read i ns tructio ns befofe comp l eting thiS f orm. PARAMETER L>< QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TIPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE .......... ........... 7.0 . ......... 7.8 0 12/28 GRAB ... 12 MEASUREMENT 00400 1 0 0 PERMIT ........... ******** -6.0 ******** 9.0 su THREE! GRAB EFFLUENT GROSS VALUE REQUIREMENT MINIMUM MAXIMUM WEEK SCUDS, TOTAL SUSPENDED SAMPLE 75 84 ******"** 9 11 0 4/28 GRAB MEAS U REME N T 26 19 00530 1 0 0 PERMIT 380 1250 LBS/DY *"it****" 30 100 MGR.. WEEKLY GRAB EFFLUENT GROSS VALUE REQUIREMENT MOAVG DAILYMX MOAVG DAILYMX OIL AND GREASE SAMPLE <52 <57 ******** <6 <6 0 4/28 GRAB MEA S UREMEN T 26 19 00556 1 0 0 PERMIT 190 250 LBS/DY .......... 15 20 MGR.. WEEKLY GRAB EFFLUENT GROSS VALUE REQU I REMENT MOAVG DAlLY MX MOAVG DAILYMX FLOW , IN CONDUIT OR THRU SAMP L E 0.960 1.110 ******** ******** ......... ** 0 28/28 TOTALZ 03 ... MEASUR E MENT 50050 1 0 0 PERMIT REPORT REPORT MGD ******** ******** *****"*** .. SEE TOTALZ EFFLUENT GROSS VALUE REQU I REMENT MOAVG DAlLY MX PERMIT SAMP L E MEASUREMENT PERMIT REQU I REMENT SAMPLE M EA SU REMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQU I REMENT NAME!nTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law lhat this d OOJrnent and all allachments were prepar ed under my r-1 '-, TELEPHONE DATE direction or supetVision in aa: oniance wilh a system des ogned to assu-e thai qualified personnel M i chae l D. Skaggs prope<1y gather and evakla:e the informallon submitted. Based on my inquiry of the pef'SOfl or persons who manage the system, or those persons directly responsille lor ga!hering the Sequoyah s::1J Pres i dent information. the onformalion subn1tted is

  • to the best of my knowledge and belief. true. accurate, 423 843-7001 11 03 05 Sequoya h S it e Vi c e Pres i dent and complete I am awar e that there are pena l ties lor stbm1t1Jng false informaoon.

S I GNATURE OF PRINCIPAL EXECUTIVE I InCluding lhe possibollly of and Wnprisonmenllor knowing violations OFF I CER OR AUTHORIZED AGENT NUMBER TIPED OR PRINTED YEAR MO DAY COMMENTS AND EXPLANATION OF AN Y VIOLATIONS l l?e ference all attachments here EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Inclu de Faa7itv NarneA..ocation if Different) NATIONAL POU.liTANT DISCHARGE ELIMINATION SYSTEM (NPDES} DISCHARGE MONITORING REPORT (DMR} MAJOR (SUBR 01) Fonn APProved. OMB No. 2040.()(1()4 . _________ _ ______ _ 64-u-M 5_so_ER-----tll DISCHARc; I wAsTE PoNo ATTN: stephanie A. Howard PARAMETER X QUANTITY OR LOADING AVERAGE MAXIMUM UNTTS MINIMUM PH SAMPLE MEASUREMENT

            • "*'*
              • '*

.. 00400 1 0 0 PERMTT **"******

              • '*

-6.0 EFFLUENT GROSS VALUE REQUIREMENT MINIMUM SOLIDS , TOTAL SUSPENDED SAMPLE .............. ...........

                • ... MEASUREMENT 00530 1 0 0 PERMTT ******** ******'**

11nHI. ............. ... EFFLUENT GROSS VALUE REQUIREMENT OIL AND GREASE SAMPLE ............. ........... * ..... ***"* .. ... MEASUREMENT 00556 1 0 0 PERMTT ******** ******** ..... ******** EFFLUENT GROSS VALUE REQU I REMENT PHOSPHORUS, TOTAL (AS P) SAMPLE ..........

                • ****'****

.. MEASUREMENT 00665 1 0 0 PERMTT ******** ...,...,.,. ..... ******'** EFFLUENT GROSS VALUE REQUIREMENT COPPER, TOTAL (AS CU) SAMPLE ******** **'******

    • "'*****'*

... MEASUREMENT 01042 1 0 0 PERMIT ******** ******** -***-* EFFLUENT GROSS VALUE REQUIREMENT IRON, TOTAL (AS FEI SAMPLE ******** ............ --.. MEASUREMENT 01045 1 0 0 PERMTT ...........

        • -** .... ***-**" EFFLUENT GROSS VALUE REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE ...............

03 TREATMENT PLANT MEASUREMENT 50050 1 0 0 PERMIT REPORT REPORT MGD ******** EFFLUENT GROSS VALUE REQUIREMENT MOAVG DAILY MX NAMEmTLE PRINCIPAL EXECUTIVE OFFICER 1 Cerufy Lnfer penalty or law 11\allhis doa.ment and aU atlachmenls were prepared under my direc:bon or supennsion in accc<dance Wllh a system designed to assure lhal qualifoed personnel M ich ael D. Skaggs property gather and evakJate lhe onlormalion submitted. 6ased en my of lhe person or persons who manage lhe system. or those persons a.-eclly responsilla '"' gathenng lhe Sequoyah S i te Vice Pres i dent mfonnattcn. lhe trlormallon sobmtled is

  • to lhe bes1 or my knowledge and belief, ltUe. acarale. and complete I am aware 11\at lhere are signifocant penallies fDf subnuiling false onformalion. tneiUdwlg lhe poSStbilily of ftne and imprisonment for l<ncMvlg Y>Oiabons TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No D i scharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used EFFLUENT pay 28 NO DISCHARGE I XX I m NOTE: Read instructions before comp leti n!llhis fonn. QUALITY OR CONCENTRATION N O. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNTTS ANALYSIS ******** 12 **"""'***

9.0 su DAILY GRAB MAXIMUM *"******* 19 ******** 30 MG.IL DAILY COMPOS DAILYMX ***'***** 19 ***""**'** 15 MGIL DAILY GRAB DAILYMX ******** 19 *****"* 1.0 MGIL DA ILY COMPOS DAILYMX ******** 19 ............. 1.0 MGIL DAIL Y COMPOS DAILYMX **"'*"***'" 19 ******** 1.0 MGIL DAI L Y COMPOS DAILYMX -... ........... .. ******** ......... -DAILY CALCTO \ TELEPHONE DATE 423 843-7001 11 03 OS SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY Page 1 of 1 PERMITTEE NAM E/ADDRESS (Inclu de F acility N a meA..ocation if Dtfferent) NATIO NAL POULITANT DISCHARGE ELIMINATIO N SYSTEM (NPDE S) DISCHARGE MONITORING REPORT (DM R) MAJOR (SUBR01) F orm A PProv ed. OMB N o , 2040-0 00 4 &Idress _p.O.JtOX 2000 ___________ _ _______ _ DlscHARGE 1 I coouNG wATER ___ _ AnN: stephanie A. Howard PARAME T ER X QUANTITY OR LOAD ING AVERAGE MAXIMUM TEMPERATU RE , WATER DEG. SAMPLE ******** --CENTIGRADE MEASUREMENT 00010 z 0 0 PERMIT ******** ******** INSTREAM MONITORING REQU I REMENT PH SAMPLE ******** *'******* M EAS U REMENT 00400 1 0 0 PERMIT "'*******

                • MO 02 UNITS 04 DEGC .. -MINIMUM ******** ******** 6.0 EFFLUENT .... NO DISCHARGE I XX I *""* NOTE: Read ins tructi ons be for e com p l e tifl!llhi s form. QUALITY OR CONCENTRATION NO. FREDUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANA LY SIS ******** 04 .........
    • 38.3 DEGC DAILY GRAB4 DAILYMX ............ 12 ...........

9.0 su WEEKLY GRAB EFFLUENT GROSS VALUE REQUIRE M ENT MINIMUM MAXIMUM SOLIDS , TOTAL SUSPENDED SAMP LE ******** **"**"""" ******** -M EASU R EM E NT 00530 1 0 0 PERMIT ******** ******** -*"***"** EFFLUENT GROSS VALUE REQUIREMENT OIL AND GREASE SAMPLE ******** ******** ******** -MEASUR EM ENT 00556 1 0 0 PERMIT **"***** ****"**** -******** EFFLUENT GROSS VALUE REQU I REMENT FLOW, IN CONDUIT OR THRU SAMPLE ******** 03 TREATMENT PLANT MEA S UREMENT 5005 0 1 0 0 P ERMIT REPORT REPORT MGD *******"* EFFLUENT GROSS VALUE REQUIREMENT MOAVG DAILY MX CHLORINE , TOTAL RESIDUAL SAMPLE *******"* *---MEAS U REMENT 50060 1 0 0 PERMIT ............ *****"*** -"*****"*** EFFLUENT GROSS VALUE REQUIREMENT SAMPLE MEASUREMENT PERMIT R E QUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER M i chae l D. Skaggs I Certtfy under penally or law thal this dowment and all a Uadvnenls were prepar ed under my '"'coon or supervtsiOO in accordance with a system designed to assure that qual i fied person-lei rty gathet and lhe rrlormatioo SllbrniHed. Based m my nq'-*y of the person or s ..no manage the system. 0< those persons diredly responsible for gathering the ***'*****

                • 30 DAILYMX ******** *******"*

15 DAILYMX ******** ***"*****

                • *****"***
                • ******** 0.10 DAILYMX ni01T11alion.

the IAfonnalion stAmitted is , lo the best of my knowledge and belief, true, aocuate, 1-----------==------ ---4 compl e te. 1 a m aw a r e that there are signlfocanl penalties f a< sU>rrnlling fal se inlormaltOn, Sequoyah S i te V i ce President it:$res i dent Sequoyah SIGNATURE OF PRINCIPAL EXECUTIVE f----------------lincluding the affine and impOsonment I O< knowing violalions. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATION S (R e Fer e nce all attachments h e r e No Discharge this Pe ri od EPA Fonn 3320-1 (REV 3199) Previous editions may be used OFFICER OR AUTHORIZED AGENT 19 MG/l DAILY COMPOS 19 MG/l DA I LY GRAB .. ...,. DAILY CALC TO 19 MG/l WEEKLY GRAB-4 TELEPHONE DATE 423 843-7001 11 03 05 I NUMBER Y EAR MO DAY Page 1 of 1 PE RM ITTEE NAM E/ADDRESS (lncJud e F a cility N a meA.ocation ff D i ff erent) .N_ame __ TVA_:_ SEOUOYAH __ ---_ ---____ _ NATIONAL POLLUTANT DISCHARGE EUMINATIO N SYSTEM (NPD E S) D I S C HAR G E MON IT O R IN G REP O R T (DM R) MAJOR (SUBR 01) E FFL U ENT F orm A pproved_ OMB N o. 2 04 0-0 0 0 4 ATTN: stepha n ie A. H o wa rd I r:v T r iNG M O D AY From I 01 ... NO DISCHARGE I XX I ..._ NOTE: Read instructio ns be for e co m pl e ti ng lhis f orm_ PARA M ETER X I C 2 5 S T A T RE 7 DA Y CHR SAMPLE CERIODAPHNIA MEASURE M ENT 1 0 0 PERMIT EF FL UENT G ROSS VALUE REQUIREMENT I C25 S T A T RE 7DA Y CHR SAMP L E PIM E PHA LES M EASUR EM ENT ITRP6C 1 0 0 PERM IT EFFLUENT GROSS VALUE REQUIREMEm SAMPLE M EA S U REM ENT P ERM I T REQUIREMENT SAM PLE M EASUREMENT P ERMIT R EQU I REMENT SAMP LE M EA SU R EME NT PERMIT REQUIREMENT SAMP LE MEAS UREM ENT PERM IT R E QUIREM E NT SAM PLE MEAS U REMENT PERMIT R E QUIREMENT NAME/TITL E PR IN C I PA L E XECUTIV E OF F ICER Michael D. S k aggs QUANTITY OR LOADING OUAUTY OR CONCENTRA TI O N A V ERA G E MAXIMUM UNITS MINIMUM AV E RAG E MAXIMUM ******** ******** ... ******** ******** ..........

    • '******

..... 45.2 ........ * . ....... MINIMUM ******** .............

                • ........... ... ******** ..... ***. *-45.2 ******** ******** MI N IMU M Sequoyah S i: '6.D P res i dent Sequoyah S it e Vice President 1 Ce<tify under penalty of law that this docoolent and all allactvnenl s we<e prepared under my diredion or supervision in accordance with a system designed l o assure tha i quaf lfi ed petsonnel property gather and evaluate the onfonnation SLtlm!tt ed. B ased on my onquiry ollhe petson or persons who manage ll1e system, or those persons d irectly responsi>le f or IJalhering the irtonnation, ll1e onlonnatial sobm ilt ed i s
  • co the best ol my knowledge and bel i ef. true. accurat e. and canplete. I am aware thallh er e are signifiCatll penalties f or sli>mi t ting f alse i nformat i on. 1------------------l SIGN ATU R E OF PR I N CI PA L E XE C UTIV E l-----------------1ncluding ll1e possobi ti ty of fine and n.>risorvnent for knowing viOlations. TYP ED OR P RINTED COMMEN T S AND EXPLANATION OF ANY V IO LATIONS £Refer e n ce all a ttJchments h e r e No D i sc harge th i s Period E PA Fo rm 3 32 0-1 (REV 3/99) Previous editions may be used O FF IC E R OR AUTHORI ZED AG E NT NO. F REQUENCY SAMPLE EX OF TYPE UNITS A N A LY S I S 23 PERCEN T S EMI COM P O S A NN U AL 23 PERCE N T S E MI COM P OS A N NUAL D ATE 42 3 84 3-7001 11 0 3 05 NUM B ER YEAR MO D AY Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/l ocation ff Different} Md re ss_p....Q.J!..OX 2.2Q(L __________

_ ___ _ NATIONAL P OLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ DISCHARGE MONITORING REPORT (DMR) DlscHARc: I MAJOR (SUBR01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB N o. ATIN: stephanie A. Howard I t:!Tr iNG ..... NO DISCHARGE D PARAMETER >< DEBRIS, FLOATING (SEVERITY) SAMPLE MEASUREMENT 01345 1 0 0 PERMIT EFFLUEN T GROSS VALUE REQUIREMENT OIL AND GREASE VISUAL SAMPLE MEASUREMENT 84066 1 0 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEITmE PRINCIPAL EXECUTIVE OFFICER Michael D. Skaggs OUANTITY OR LOADING AVERAGE MAXIMUM ******'**

                • ..........
    • "***** ******** 0 ****'****

REPORT MOTOTAL NOTE: Read i nstructions before romplelinl:l this fomt. OUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE UNITS MINIMUM AVERAGE MAXIMUM UNITS ANAlYSIS ..... ..._ '******** 0 0 1 I 28 VISUAL -9A **** ******** *******'* REPORT PASS=O SEE VISUAL MOTOTAL FAll=1 PERMIT ******** '******'** ............. 0 1 I 28 VISUAL 94 ** YES=1 ******** ******** ******** .... SEE VISUAL NO:O PERMIT ' TELEPHONE DATE Sequoyah S i te Vice PresiOent I Cerufy under penalty of law lha l this doaJmenl and a ll attachments were prepared Wlder my direc:lion or in accordance With a system designed to assu-e thai qualifoed personnel gather and eva!ua\e the onfonnalion submitted. Based on my of the person or who m;nage the syslem. ex those persons <Wectly responsible for gathemg the informabOn. the onforma6on S<bnltled is

  • ID the best of my knowledge and belief , lnJe. accuate, and complete.

I am aware ltlal there are signofocanl penalties lex submilling false infonnalion. 1----:--:-:--:-::-----:-::-::-::-::----:------:-----i Sequoyah Sit w: es i dent SIGNATURE OF PRINCIPAL EXECUTIVE 423 843-7001 11 03 05 1------------------;induding the possibilay or f111e and ""risorvnent l ex knowing VJOiations TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS f Refer e nce all attachments h ere Operat io ns performs visual inspections for floati ng deb ris and oil and grease du ring all backwashes. EPA Form 3320-1 (REV 3199) Pre viou s ed itions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include FaCifitv Name/location 1f DifferenO _________ _ _ _ _ _ _____ -- FaQ!lt'L_ _TVA..:_SEQUOYAii!-.IUC!....E_AR PLANT ____ _ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPD£5) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 0 1) EFFLUENT Form ApProved. OMB No. 2040-0004 ATTN: stephanie A. Howard QAY 28 -NO DISCHARGE D -PARAMETER >< QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM DEBRIS, FLOATING (SEVERITY) SAMPLE .............. .........

        • '****

-MEASUREMENT 01345 1 0 0 PERMIT ******** ******** ....... "*'**"*** EFFLUENT GROSS VALUE REQUIREMENT OIL AND GREASE VISUAL SAMPLE ******** 0 ........... 94 MEASUREMENT 84066 1 0 0 PERMIT ******** REPORT YES=1 ******** EFFLUENT GROSS VALUE REQUIREMENT MOTOTAL NO=O SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMP LE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/T ITLE PRINCIPAL EXECUTIVE OFFICER I Cert.\fy oodet penalty of law thai this <lo<:oolent and all atladwnents were prepared o.nlet my i---=-::.::.=..:..:.:-==..:....:.:.::..:..::::..:c.:..=..=.:::.=:...:..:...c=....=..:....:...:..::.=:..-id.r .eciJOn or supernsoon on acoordance With a system designed lo assure that qualified personnel Michael D. Skaggs propel1y ga1her and evaluate the riormalion Slbnitted Based on my onqwry of !he person or persons who manage lhe system. or those persons directly responsible for galhering lhe NOTE: Read instruction s before complelill!llhis loon. QUALITY OR CONCENTRATION NO. FREQUENC Y SAMPLE E.X OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS ........... 0 0 1/28 VISUAL 9A ****11:*** REPORT PASS=O SEE VISUAL MOTOTAL FAIL=1 PERMIT ******** ******** 0 1/28 VISUAL .. ******** ......... .... SEE VISUAL PERMIT TELEPHONE DATE Sequoyah 423 843-7001 11 03 05 ll"lformanon, the nonnation submitted is

  • to the best of my knowledge and behef. true. 1----------------l complete I am aware that !here are sognifocant.

penalties for submlllong false onformaliorl. Sequoyah Site Vice Pres iden t SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED A GENT NUMBER YEAR MO DAY !he possibility orr.,., and mprisoMienl for Mowing VIOlations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Refe ren ce all attachments here Operations performs visual inspec tions fo r floating debris and oil and grease du ring all ba ckwash es. EPA Fonn 3320-1 (REV 3/99) Prewous editiOns may be used Page 1 of 1 PERMITTEE NAME/A DDRESS (I nclu d e F aa1itv Na meA.ocat ion if D1H eren t} NATl ONAI. POUliT ANT DISCHARGE EU M t NA TlO N S Y STEM (NPDE S} D I SCHAR G E M O N IT O RI NG REPORT (DMRJ MAJOR (S U BR 0 1) Form App r oved. OMB N o. 2040-0004 Add r ess __P...Q._!!_O X 2 00Q_ __________ _ 64-u-M 5-s 0-ER---ill o1scHARa; I & s T o RM w A T E R ___ _ _________ _ A TIN: st e phan ie A. Ho w ard I ..... ;g"+:_...M.w...O +-D"""'p.y'"'""l E F FLUE NT From I [ 01 ] TO 1.__;1_1_}...__0'-2___..---"-2-'-8__, ..... NO DIS CHAR GE NOTE: Read in stru ction s before completinq lh i s form. l xx I ..... PARAMETER X QUANTITY OR LOADI N G QUALITY OR CONCENTRATION NO. FR EQ UENCY SAMPLE EX O F nPE AVERAGE M AX IMUM UNI T S MI N IMU M A VERAGE MAXIMUM UNITS ANAL Y SIS O X YGEN , DISSOLVED (DO) SAMPLE ******** ................... ................

                • MEASUREMENT

... 19 00300 1 0 0 PERMIT ******** ******** -2.0 ******** .......... M G IL TW ICE/ GRAB EFFLU E NT G R O S S V ALU E REQUIREMENT DAILYMN WEE K SOLIDS , TOTAL SUSPENDED SAMP L E *"*******

                • **'****"** ******** ... 1 9 MEASUREMENT 00530 1 0 0 PERMIT ******** ..........
  • -......... ........... 100 MGJL TW IC E/ GRAB EF FL U E N T GROSS VALU E REQUIREMENT DAILY MX WEE K SO LI DS , SETTLEABLE SAMPLE ******** ******** ******** **'*"*****

... 25 MEAS U REMENT 00545 1 0 0 PERMIT ......... ******** .... ******** ******** 1.0 M lll O NC E/ G RA B EFFLUENT GROSS VA L U E REQ U IREMENT DAILYMX M O NTH F L OW , IN CO N DUIT OR THRU SAM PLE **'******

                • ******"""*

03 .. jT REA TM E NT PLAN T MEASUREMENT 50050 1 0 0 PERMIT REPORT REPORT MGD ................ _ ... ***"***** . ON CE/ E STl MA EFFLU E NT G R OSS VAL UE REQUIREMENT MOAVG DAILY MX BA TCH SAMPLE MEA S UREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT R E QUI R EMENT SAMPLE MEASUREMENT PERMIT RE Q UIREMENT I NAME/TITl E PRI NC I PAL EXEC U TIVE O FF ICER I Certify U'lder penalty of law tha t lhos docunenl a nd al l allachnenls were pr e pared ooder my TELE PHO NE edion or supe<vislon Vl accordance Wllh a system designed to assure that quafofied persomel M i chael D. Skaggs ""operty g a ther and evatuale lhe llllormallon submitted. Ba sed on my lllqUiry of lhe perso n or person s wf1o m ana ge the syst em , or those persons directly responsible f or ga lherWig lhe irlormaiJon. lhe information StJbmitt ed is

  • to lhe best or my knowledge and befoef , ttue, acx:uale, 4 23 843-7 001 1 1 0 3 05 Se q uoyah S i te Vice P res i dent and oomplele I am ;rware that there a re sognilicant penalties f or subrnillJtlg false irtormabon, SIG NA TU RE O F PR INCI PA L EXEC UTIVE I oncluding lhe po ssi bility of fine a nd impnsonment for kno w n>g VIOlations.

OF F ICER O R AUTtJORIZE D A GENT :: I N U M BE R YEA R M O D AY n P E D O R PRI NTE D COM ME N T S AN D O F ANY f Reference a ll attachments he r e During th is r e porting pe ri od. there has be e n no fl o w f rom t h e Dredge Pond other tha n tha t resu l ting f r om r a in fa ll. E PA Fo rm 3 32 0-1 (REV 3 199) P reviou s edition s may be u sed Pag e 1 of 1 REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-February 2011 DMR ORGANIZATION: Environmental DOCUMENTPREPAREDBY

BrndLove DATE: 03/5/2011 CONCURRENCES Name R c Signature

-Comment Date v N B.M. Love X ...g_ {oS/ZO t\ '<._.../ S. A. Howard X ('( (<--{ c I D.A. Day X (fJ. ()Q 3/7 j11 ' M.D. Skaggs X u ?/1/11 U() INSTRUCTIONS

Originator will determine the r eview/concurrence assignment.

REVIEW: Exam i ne technical content and commitments made. A review (RV) should confirm t he truth and accuracy of factual statements and ind i cate agreement with comm i tments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose , logical in construction , and clear in meaning in the eyes of the recipient. A concurrence signatu r e i nd i cates that the individual would be w i lling to s i gn the do c ument for the agency. S58 110412 800-NPDES CORRESPONDENCE April12 , 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor , L & C Annex 401 Church Street Nashville , Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR MARCH 2011 Enclosed is the March 2011 Discharge Monitoring Report for Sequoyah Nuc l ear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 3/19/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supeNision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system , or those persons directly responsible for gathering the information , the information submitted is , to the best of my knowledge and belief, true , accurate , and complete. I am aware that there are significant penalties for submitting false information , including the possibility of fine and imprisonment for knowing violations. Sincerely , Michae l D. Sk]25 Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building , Suite 550 540 McCall i e Avenue Chattanooga , Tennessee 37402-2013 B. E. Brickhouse , LP 5U-C G. M. Cook , OPS 4A-SQN D. A Day , POB 2A-SQN S. A Howard , OPS 5N-SQN K. Langdon , POB 2B-SQN U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington , DC 20555 D. B. Nida , LP 5U-C A. A. Ray , WT 11A-K G. R. Signer , WT 6A-K M. D. Skaggs , OPS 4A-SQN K. M. Hodges (EDMS), LP 2V-C Tennessee Valley Authority, Post Office Box 2000. Soddy Daisy , Te nnessee 37384-2000 April12, 2011 State of Tennessee Department of E n vironment and Conservation D i v i sion of Wate r Pollut i on Cont r o l Enforcement & Compliance Sect i on 6 1 h Floor , L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patr i ck Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR MARCH 2011 Enclosed i s the March 2011 Discharge Monitor i ng Report for Sequoya h Nuc l ear Plant Samp l e collection continues at the Diffuser Pond I n l et of the Yard Drainage Pond effluent because of a tra n sformer oil sp i ll that reached the Yard Drainage Pond. Samples collected fr o m 9/23/2010 through 3/1 9/20 1 1 have all yie l ded resu l ts below detection l i mits for oi l and grease. If you have any ques t ions or need additional information , please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system , or those persons directly responsible for gathering the information , the information submitted is, to the best of my knowledge and belief, true , accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. S i ncerely, Site Vice President Sequoyah Nuclear P l ant Enc l osure cc (Enclosure): Chattanooga Environmental Field Office Division of Water Pollution Control State Office B u i l ding , Suite 550 540 McCall i e Avenue Chattanooga , Tennessee 37402-2013 U.S. Nuclear Regulatory Comm i ssion Attn: Document Contro l Desk Washington, DC 20555 PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR} MAJOR (SUBR 01) Fonn Approved. OMB No. 2040-0004 Address_p...Q.JtOX 2QOO_ __________ _ ______ _ I DisCHARGE ___ _ EFFLUENT I pi:lgt MO DAY *** NO DISCHARGE D ... ATTN: stephanie A. Howard NOTE* Read instructions before completing this form PARAMETER [X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 28.6 0 31/31 RCORDR MEASUREMENT

    • 04 CENTIGRADE 00010 1 0 PERMIT ******** ******** **** ******** ******** Req. Mon. DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 14.0 0 31/31 MODELD ** 04 CENTIGRADE MEASUREMENT 00010 z 0 PERMIT ******** ******** **** ******** ********

30.5 DEG.C. CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAILVMX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** 1 0 31/31 CALCTD ** 04 UPSTRM DEG.C MEASUREMENT 00016 1 1 PERMIT ******** ******** **** ******** ******** 5 DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILVMX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** 1642 ******** ******** ******** 0 31/31 RCORDR 03 ** TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ******** ******** .... CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** 0.011 0.024 0 16/31 GRAB ** 19 MEASUREMENT 50060 1 0 PERMIT ******** ******** **** ******** 0.1 0.1 MG/L FIVE PER CALCTD EFFLUENT GROSS REQUIREMENT MOAVC DAILY MAX WEEK TEMPERATURE-C, RATE OF SAMPLE ******** 0 ******** ******** 0 31/31 CALCTD 62 ** CHANGE MEASUREMENT 82234 1 0 PERMIT ******** 2 DEG ******** ********

              • .... CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILVMX C/HR NUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TinE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my r1 '7f L56 TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President information. the information submitted is

  • to the best of my knowledge and belief. true, accurate.

423 I 843-7001 11 04 12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information. SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment tor knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS £Reference all attachments here No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: Biodetergent 73551 (max. calc. cone. was 0.02mg/L-Iimit 2.0mg/L) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 Mean# of Water Mean# of Water SUB NOTES:% Sample Date ZM/m3 %Settlers Temp. ("C) Sample Date Asiatic Temp.("C) LOCATION LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-ISV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB 02/08/2011 0 0 9 02/08/2011 0 9 1-25-545 MJW 02/15/2011 0 0 23 02/15/2011 0 23 1-25-545 MLW 02/22/2011 20 100 10 02/22/2011 0 10 1-25-545 PB 03/01/2011 0 0 11 03/01/2011 0 11 1-ISV-24-1236 PB 03/08/2011 0 0 11 03/08/2011 0 11 1-ISV-24-1236 WE 03/16/2011 22 0 11 03/16/2011 0 11 1-ISV-24-1234 MLW 03/23/2011 0 0 11 03/23/2011 0 11 1-ISV-24-1234 MLW 03/30/2011 0 0 12 03/30/2011 0 12 1-15v-24-1236 MLW PERMITTEE NAME/ADDRESS (Include FacilitY Name/Location if Different) Name TVA....:_ SEQUOYAH PLAN!_ __ _ Address P.O. BOX 2000 ___________ _ ______ _ Facllltv NgLEAR.PLANT ____ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) EFFLUENT Fonn Approved. OMB No. 2040-0004 ATTN: stephanie A. Howard *** NO DISCHARGE D *** NOTE: Read instructions before completing this form PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRAnON NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ******** ******** Monitoring

                • ******** ** 23 CERJODAPHNIA MEASUREMENT Not Required TRP3B 1 0 PERMIT ******** ******** **** 43.2 ******** ******** PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MINIMUM ANNUAL JC25 STATRE 7DAY CHR SAMPLE ******** ******** Monitoring
                • ******** ** 23 PIMEPHALES MEASUREMENT Not Required TRP6C 1 0 PERMIT ******** ******** **** 43.2 ******** ******** PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

_L_ NAME/TinE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 11 04 12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here Toxicity was not sampled in March 2011. EPA Fonn 3320-1 (REV 3199) Previous editions may be used OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different} Name TVA_:_ PLANT __ _ Address P...Q.BOX2Q!)O ___________ _ ___ ______ _ Facllltv _TVA..:_SEQ!JQYAH NUCLEAR PLANT ____ _ _________ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} DISCHARGE MONITORING REPORT (DMR} MAJOR (SUBR 01) EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: stephanie A. Howard *** NO DISCHARGE D *** NOTE: Read instructions before completing this form PARAMETER I>< QUANTrrY OR LOADING QUALrrY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ******** ******** 7 ******** 8 0 15/31 GRAB MEASUREMENT -12 00400 1 0 PERMIT ******** ******** ** 6 ******** 9 su THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** 14 16 0 2131 GRAB ** 19 MEASUREMENT 00530 1 0 PERMIT ******** ******** ** ******** 30 100 MGIL lWICEI GRAB EFFLUENT GROSS REQUIREMENT MOAVO DAILYMX MONTH OIL AND GREASE SAMPLE ******** ******** ******** <5 <6 0 2131 GRAB ** 19 MEASUREMENT 00556 1 0 PERMIT ******** ******** ** ******** 15 20 MGIL lWICEI GRAB EFFLUENT GROSS REQUIREMENT MOAVO DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 0.964 1.089 ******** ********

                • 0 31/31 RCORDR 03 ** TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD ******** ******** *********

.. SEE RCORDR EFFLUENT GROSS REQUIREMENT MOAVO DAILYMX PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 11 04 12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of line and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO TIPED OR PRINTED DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facilitv NameA..ocation if Different} Name __ TVA_:_ SEOUOYAH PLAN!_ __ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM {NPDES} DISCHARGE MONITORING REPORT {DMR} MAJOR (SUBR 01) Fonn Approved. OMB No. 2040-0004 AddressJ..Q.JtOX 2000 ___________ _ Facllltv _TVA..:_SEQUOYAH N.!,K:LEAR.PLANT ____ _ MQ ATTN: stephanie A. Howard 03 PARAMETER >< QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** MEASUREMENT

    • CENTIGRADE 00010 1 0 PERMIT ******** ******** -******** EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** -CENTIGRADE MEASUREMENT 00010 z 0 PERMIT ******** ******** -******** INSTREAM MONITORING REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** -UPSTRM DEG.C MEASUREMENT 00016 1 0 PERMIT ******** ******** -******** EFFLUENT GROSS VALUE REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** MEASUREMENT 03 TREATMENT PLANT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** EFFLUENT GROSS VALUE REQUIREMENT DAILYMX CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** ** MEASUREMENT 50060 1 0 PERMIT ******** ******** ** ******** EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE-C, RATE OF SAMPLE ******** ******** 04 CHANGE MEASUREMENT 82234 1 0 PERMIT ******** 2 DEGC ******** EFFLUENT GROSS VALUE REQUIREMENT DAILYMX SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachmenls were prepared under my direction or supervision in accordance wilh a system designed to assure that qualified personnel Michael D. Skaggs property gather and evaluate the informalion submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violalions. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No Discharge this Period. EPA Form 3320*1 (REV 3/99) Previous editions may be used EFFLUENT *** NO DISCHARGE I XX I *** NOTE: Read instructions before completing this fonn QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS ******** 04 ******** REPORT DEGC CONTIN CALCTD DAILYMX uous ******** 04 ******** 30.5 DEGC CONTIN CALCTD DAILYMX uous ******** 04 ******** 5 DEGC CONTIN CALC TO DAILYMX uous ******** ******** ** ******** ******** .. CONTIN RCORDR uous 19 0.1 0.1 MGIL Five per CALC TO MOAVG DAILYMX Week ******** ******** ** ******** ******** .. CONTIN CALCTD uous I I TELEPHONE DATE 423 843-7001 11 04 12 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA_:_ SEOUOYAH PLAN!_ __ _ NATIONAl POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Address P.O.JiOX 200Q_ __________ _ ______ _ ______ _ ___ _ A1TN: stephanie A. Howard PARAMETER X IC25 STATRE 7DAY CHR SAMPLE CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT IC25 STATRE 7DAY CHR SAMPLE PIMEPHALES MEASUREMENT TRP6C 1 0 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT I YEAR MO e;:;gt MO DAY From 11 I 03 01 To RECYCLED COOLING WATER EFFLUENT *** NO DISCHARGE I XX I *** NOTE: Read instructions before completing this form QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY EX OF AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS ******** ******** ******** ******** -23 ******** ******** **** 43.2 ******** ******** PERCENT SEMI MINIMUM ANNUAL ******** ******** ******** ******** -23 ******** ******** -43.2 ******** ******** PERCENT SEMI MINIMUM ANNUAL . SAMPLE TYPE COMPOS COMPOS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law !hat this document and all attachments were prepared under my . TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submilled. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submilled is , to the best of my knowledge and belief, true, accurate, Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submilling false information, including the possibility of fine and imprisonment for knowing violations. OR PRINTED COMMENTS AND OF ANY (Reference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 843-7001 11 04 12 I NUMBER YEAR MO DAY Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different) Name TVA_:_ PLAN!_ __ _ Address P.O . ...R.OX 2000 ___________ _ ______ _ Facilitv TVA..:.$EQUOYAH NUCLEAR PLANT ____ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} DISCHARGE MONITORING REPORT (DMR} MAJOR (SUBR 01) EFFLUENT Fonn Approved. OMB No. 2040-0004 ATIN: Stephanie A. Howard *"* NO DISCHARGE I XX I **" NOTE: Read instructions before completing this form PARAMETER IX QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM OXYGEN, DISSOLVED (DO) SAMPLE ******** ******** ******** ******** -MEASUREMENT 00300 1 0 PERMIT ******** ******** *"** 2 ******** ******** EFFLUENT GROSS REQUIREMENT MINIMUM SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 00530 1 0 PERMIT EFFLUENT GROSS *REQUIREMENT SOLIDS, SETILEABLE SAMPLE MEASUREMENT 00545 1 0 PERMIT EFFLUENT GROSS REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT EFFLUENT GROSS REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Michael D. Skaggs ******** ******** ******** ******** ** ******** ******** *"** ******** ******** 100 DAILYMX ******** ******** ******** ******** ** ******** ******** **** ******** ******** 1 DAILYMX ******** ********

                • 03 Req. Mon. Req. Mon. MGD ******** ******** ******** MOAVG DAILYMX Sequoyah Site Sequoyah Site Vice President I Certify under penalty of Jaw that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, 1--::::=-:-::-=:-=-=-::-::-::-:--::::-::-------l SIGNATURE OF PRINCIPAL EXECUTIVE OR PRINTED f-----------------l'including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Previous editions may be used NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 19 MG/L lWICE/ GRAB WEEK 19 MG/L lWICEI GRAB WEEK 25 MUL ONCE/ GRAB MONTH **

  • ONCE! ESTIMA BATCH TELEPHONE DATE 423 843-7001 11 04 12 NUMBER YEAR MO DAY Page 1 of 1 REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-March 2011 DMR ORGANIZATION:

Environmental DOCUMENT PREPARED BY: Brad Love DATE: 04/11/2011 CONCURRENCES Name R c Signature -Comment Date v N B.M. Love X S. A. Howard X D.A. Day X M.D. Skaggs X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. S58 120201 802-NPDES CORRESPONDENCE February 1 , 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor , L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-CORRECTION TO DISCHARGE MONITORING REPORT FOR MARCH AND APRIL 2011. During the review of the Biocide/Corrosion Treatment Plan (B/CTP) Annual Report it was noted that there was an error reported for Frequency of Analysis in the March and April 2011 Discharge Monitoring Reports (DMR). Please see the attached corrected DMR pages. It should be noted that the chlorine , total residual maximum value and the chlorine , total residual average did not change as a result of the correction to the Frequency of Analysis for each month. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system , or those persons directly responsible for gathering the information , the information submitted is, to the best of my knowledge and belief, true , accurate , and complete. I am aware that there are significant penalties for submitting false info rmation , including the possibility of fine and imprisonment for knowing violations. rlin 1 e jce President Seqtfoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building , Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 B. E. Brickhouse. LP 5U-C J. T. Carlin , OPS 4A-SQN G. M. Cook , OPS 4A-SQN J. A. Cross, POB 2A-SQN S. W. Hixson , BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 D. B. Nida , LP 5U-C A. A. Ray , WT 11A-K G. R. Signer , WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1 E-M Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 February 1, 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT (SQN)-NPDES PERMIT NO. TN0026450-CORRECTION TO DISCHARGE MONITORING REPORT FOR MARCH AND APRIL 2011. During the review of the Biocide/Corrosion Treatment Plan (B/CTP) Annual Report it was noted that there was an error reported for Frequency of Analysis in the March and April 2011 Discharge Monitoring Reports (DMR). Please see the attached corrected DMR pages. It should be noted that the chlorine, total residual maximum value and the chlorine, total residual average did not change as a result of the correction to the Frequency of Analysis for each month. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F-FINAL Form Approved. OMB No. 2040-0004 Address_ ...f..O. BOX 20QQ.. ________ .:..._ __ _ TN0026450 PERMIT NUMBER ____ _ ATTN: Brad Love PARAMETER X QUANTITY OR LOADING AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ***'*****

    • CENTIGRADE 00010 1 0 PERMIT ******** ********

-EFFLUENT GROSS REQUIREMENT TEMPERATURE, WATER DEG. SAMPLE ******** ******** ** CENTIGRADE 00010 z 0 PERMIT ******** ******** -INSTREAM MONITORING REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ** UPSTRM DEG.C 00016 1 1 PERMIT ******** ******** -EFFLUENT GROSS REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE ******** 1642 03 50050 1 0 PERMIT ******** Req. Mon. MGD EFFLUENT GROSS REQUIREMENT DAILY MAX CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ** 50060 1 0 PERMIT ******** **-*** -EFFLUENT GROSS REQUIREMENT TEMPERATURE-C, RATE OF SAMPLE ******** 0 62 CHANGE 82234 1 0 PERMIT ******** 2 DEG EFFLUENT GROSS REQUIREMENT DAILYMX C/HR SAMPLE PERMIT REQUIREMENT MINIMUM ******** ******** ******** ******** ******** ******** ******** ******** ******** ******** ******** ******** / DIFFUSER DISCHARGE EFFLUENT *** NO DISCHARGE D*** NOTE: Read Instructions before completin!l this form QUALITY OR CONCENTRATION NO. FREQUENCY EX OF AVERAGE MAXIMUM UNITS ANALYSIS ******** 28.6 0 31/31 04 ********* Req. Mon. DEG.C. CONTI DAILY MAX NUOUS ******** 14.0 0 31/31 04 ******** 30.5 DEG.C. CONTI DAILYMX NUOUS ******** 1 0 31/31 04 ******** 5 DEG.C. CONTI DAILYMX NUOUS ******** ******** ** 0 31/31 ******** ******** .... CONTI NUOUS 0.011 0.024 19 0 15/31 0.1 0.1 MGIL FIVE PER MOAVG DAILY MAX WEEK ******** 0 31/31 ** ******** ******* .... CONTI NUOUS ,\-/' I . / SAMPLE TYPE RCORDR CALCTD MODELD CALC TO CALCTD CALCTD RCORDR RCORDR GRAB CALCTD CALCTD CALCTD NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all aHachments ware prepared under my TELEPHONE DATE direction or supervision in accordance with a system designad to assure that qualifiad parsonnal John T. Carlin properly gather and evaluate tha information submlttad. Based on my inquiry of tha parson or persons who manega the system, or those persons directly responsible for gather1ng the resident Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 12 01 17 and complete. I am aware that there are significant penalties for submitting false Information, PRINCIPAL EXECUTIVE I Including the possibility of fine and imprisonment for knowing violations. OFF R OR AUTHORIZED AGENT AREA I NUMBER MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: Biodetergent 73551 (max. calc. cone. was 0.02mg/L-Iimit 2.0mg/L) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location ff Different) ___ _ Addres.!_ ....f..Q,_BOX 2000 ___________ _ ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) 101 G MAJOR (SUBR 01) F-FINAl DIFFUSER DISCHARGE EFFLUENT *** NO DISCHARGE o--Form Approved. OMB No. 2040-0004 NOTE* Read instructions before completlna this form PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE AVERAGE MAXIMUM UNITS MINIMUM TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ** CENTIGRADE 00010 1 0 PERMIT *********

                • ---EFFLUENT GROSS REQUIREMENT . TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ** CENTIGRADE 00010 z 0 PERMIT ******** ******** -******** INSTREAM MONITORING REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ** UPSTRM DEG.C 00016 1 s PERMIT *********
                • -******** EFFLUENT GROSS REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE ******** 1642 ******** 03 TREATMENT PLANT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** EFFLUENT GROSS REQUIREMENT DAILY MAX CHLORINE, TOTAL RESIDUAL SAMPLE
                • ******** ** **1t*****

50060 1 0 PERMIT ******** ******** -******** EFFLUENT GROSS REQUIREMENT TEMPERATURE-C, RATE OF SAMPLE ******** 1 ******** 62 CHANGE 82234 1 0 PERMIT ******** 2 DEG ******** EFFLUENT GROSS REQUIREMENT DAILYMX C/HR SAMPLE PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin I Certify under penally of Jaw that this document and all attachments were prepared under my direction or supervision In accordance with a system designed to assure that qualified personnel properly gather and evaluate the lnfonnalion submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the AVERAGE MAXIMUM ******** 34.4 ******** Req. Mon. DAILY MAX ******** 19.7 ******** DAILYMX ******** 3 ******** 3 DAILYMX ******** ******** ******** ******** 0.017 0.026 0.1 0.1 MOAVG DAILY MAX ******** ******** ******* President information, the information submitted is , to the bast of my knowledge and belief, true, accurate, Sequoyah Site Vice President and complete. 1 am awsre that there are significant penalties for submitting false Information, EX OF TYPE UNITS ANALYSIS 04 0 30/30 RCORDR DEG.C. CONTI CALCTD NUOUS 04 0 30/30 MODELD DEG.C. CONTI CALC TO NUOUS 04 0 30/30 CALCTD DEG.C. CONTI CALC TO NUOUS ** 0 30/30 RCORDR -CONTI RCORDR NUOUS 19 0 25/30 GRAB MGIL FIVE PER CALCTD WEEK ** 0 30/30 CALC TO -CONTI CALCTD NUOUS TELEPHONE DATE 423 843-7001 12 01 17 1------:=-===--==-=====-------tincluding the possibility of fine and Imprisonment for knowing violations. r-:-AR=EA=-+--N""U""M:-=BE=-R=-. -tc-Y-EA-R-t-..,-M-O-f-D-A-Y-l TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. Veliger Monitoring Data is includes as an attachment. The following injections occurred:

1. Towerbrom 960 2. Spectrus CT1300 (max. calc. cone. was 0.039mg/L-Iimit 0.050mg/L)

EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-March I April 2011 DMR Corrections ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 1119/2012 CONCURRENCES Name R c Signature -Comment Date v N B.M. Love X ' J.L. Grubb X J.A. Cross X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. S58 110510 800-NPDES CORRESPONDENCE May 10,2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h F l oor , L & C Annex 401 Church Street Nashville , Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR APRIL 2011 Enclosed is the April 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 4/30/2011 have all yielded results below detection li mits for oi l and grease. If you have any quest i ons or need additional informat i on , please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system , or those persons directly responsible for gathering the information , the information submitted is , to the best of my knowledge and belief, true , accurate , and complete. I am aware that there are significant penalties for submitting false information , including the possibility of fine and imprisonment for knowing violations. Sincerely , .. Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure)

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building , Suite 550 540 McCallie Avenue Chattanooga , Tennessee 37402-2013 B. E. Brickhouse , LP 5U-C G. M. Cook , OPS 4A-SQN D. A. Day , POB 2A-SQN S. A. Howard , OPS 5N-SQN K. Langdon , POB 2B-SQN U.S. Nuclear Regulatory Comm i ssion Attn: Document Control Desk Washington , DC 20555 D. B. Nida , LP 5U-C A. A. Ray , WT 11A-K G. R. Signer , WT 6A-K M. D. Skaggs , OPS 4A-SQN K. M. Hodges (EOMS), LP 2V-C Tennessee Valley Authority, Post Office Box 2000, Soddy Dai s y, Tennessee 37384-2000 May 10 , 2011 State of Tennessee Department of Environment and Conservation Division of Water Pol l ution Contro l Enforcement

& Compliance Section 6 1 h F lo or, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR APRIL 2011 Enclosed is the April 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond I nlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Dra i nage Pond. Samp l es collected from 9/23/2010 through 4/30/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information , please contact Brad Love at (423) or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supeNision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information , the information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely , .. Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure)

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building , Suite 550 540 McCallie Avenue Chattanooga , Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington , DC 20555 PERMITTEE NAME/ADDRESS (Inclu de Facilitv Namellocafion if Different)

.. J!.PX _________ _ ____ _ _________ _ NATIONAL POllliTANT DISCHARGE EliMINATION SYSTEM (NPO ES) DIS C HAR G E MONITORIN G R EP OR T (DM R) MAJOR (SUB R 01) EF F LU E NT F onn App roved. OMB No. 2040-0004 ATIN: stephanie A. H o ward I YfAR I MO 04 [ 01 ] To l._-'-1 -'-1 -']'---'-04_;__J'---'- 30-'---' NO DISCHARGE D .... NOTE: Read in structions before complelin!l this form. PA RA METER X QUANTITY OR LOADING OUALITI OR CONCENTRAT I ON NO. FREQUENCY S AMPLE E X OF TYPE AVERAGE MAXIM U M UNITS MIN IMU M AVERAG E MAXIMUM UNITS ANALYSIS !TEM PERATURE, WATE R DEG. SAMPLE ******** ******** **'****** ................. 34.4 0 3 0/30 RCOR D R -04 CE NTI GRADE MEASUREMENT 00010 1 0 P E RMIT *******"*

                • ..... *****'***
                • Req. Mon. D EG.C. CONTI CALC TD EF FL UENT GROSS REQUIREMENT DAILY MAX NU O U S TE M PE RA TURE , WA T ER DEG. SAMPLE ******** ******** ******** .. ....... 1 9.7 0 30/30 MODELD -04 CENTIGRADE MEASUREMENT 00010 z 0 PERMIT ******** ******"**

...... ******fr:*

            • "*'*

30.5 DE G.C. CONTI CALCT D INSTREAM MONITO R ING REQUIREMENT DAILYMX N UOUS T E M P. DIFF. BETWEE N SA MP. & SAMPLE **'****** -................ ...... *** 3 0 30/3 0 CALC TO -04 U PS T R M DEG.C MEASUREMENT 00016 1 s PERMIT ******** ******** .... *****fr:** ......... 3 O EG.C. CONTI CALC TO EFFLUENT GROSS REQUIREMENT DAILYMX NUOUS FLO W, I N CO NDUIT OR TH R U SAMPLE ******** 1642 ******** ..............

            • '*"*

0 30/30 RCORDR 0 3 .. TR EA TM E NT P LAN T MEASUREMENT 50050 1 0 PERMIT ***"***** Req. Mon. M GD ******** ******** ***-****"* -CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS C H L ORI N E , TOT A L R ESIDUA L SAMPLE .............

      • "*****
                • 0.017 0.026 0 28/3 0 GRAB .. 19 MEASUREMENT 50060 1 0 PERMIT ******** ******** -******** 0.1 0.1 MG/L FlVE PER CALC TO EFFLUENT GROSS REQUIREMENT MOAVG DAILY MAX WEE K !T E M PERA T URE-C , RATE OF SAMPLE ******** 1 **-****** *******'*

0 3 0/30 CALCTD 6 2 .. CHAN GE MEASUREMENT 82234 1 0 PERMIT *'******* 2 DEG ******"**

                • ******* .... C O NTI CALC TO EFFLUENT GROSS REQU I REMENT DAILY MX C/H R N U OU S SAMPLE MEASUREMENT PERMIT REQUIREMENT

./'_l_ NAME/Tin E P RINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this clocunent and all aUachments were prepared under my r-1 oz5 TELE P H ON E D A T E iredion or supetVision in a<X)()(dance with a system lo assure that quaJffied persomel M ich ae l D. Skaggs property gather and evakJate the lflforma!Jon Slbnotted Based on my inquiry ollhe person or persons who mMage the system, or those p<<SSnS directly responsible for gathenng the Sequoyah Site Vice Pres i dent 423 843-7001 11 05 09 *rlorma tion , the informaloon S<bnitled is

  • to the best or my knowledge and belief, true. accuat e. Sequoyah S i te Vice President and complete.

I a m awate lhal there are sigrl!f;cart penallies lor sltlm*lhng false information. S I GNATUR E OF P RI N C I PAL EX E CUTIVE _I tnckJdlng the possililily of line and imprisonment for knowing violations. OFfiCER OR AUTHORIZED AGENT AREA 1 NUMBER YEAR MD DAY TIPED OR PRINTED rnnF COMM E NTS AND EXPLANATION OF ANY (Reference all attachments her e No closed mode operation. Ve li ge r Monitoring Data i s includes as an attachment. The following injections occurred: 1. Towerbrom 960 2. Spectr u s CT1300 (ma x.. calc. cone. was 0.039mg/L-I i mit 0.050mg f L) EPA F orm 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 Mean# of Water Mean# of Water SUB NOTES:% Sample Date ZM/m3 %Settlers Temp. ("C) Sample Date Asiatic Temp.("C) LOCATION LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-JSV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB 02/08/2011 0 0 9 02/08/2011 0 9 1-25-545 MJW 02/15/2011 0 0 23 02/15/2011 0 23 1-25-545 MLW 02/22/2011 20 100 10 02/22/2011 0 10 1-25-545 PB 03/01/2011 0 0 11 03/01/2011 0 11 1-ISV-24-1236 PB 03/08/2011 0 0 11 03/08/2011 0 11 1-ISV-24-1236 WE 03/16/2011 22 0 11 03/16/2011 0 11 1-ISV-24-1234 MLW 03/23/2011 0 0 11 03/23/2011 0 11 1-ISV-24-1234 MLW 03/30/2011 0 0 12 03/30/2011 0 12 1-15v-24-1236 MLW 04/06/2011 18 100 15 04/06/2011 0 15 1-ISV-24-1234 HMW 04/08/2011 45 100 15.5 04/08/2011 0 15.5 1-1SV-24-1236 WAW/PB 04/20/2011 21 100 16 04/20/2011 0 16 1-1SV-24-1236 PB PERMITTEE NAME/ADDR E SS (Incl ude Fa CJ7itv Na me/location if D i ffe rent) _N!l m e __ PLA N!_ __ _ NATIONAl POLLlJTANT DISC HAR GE Eli M INATIO N SYSTEM (N PDE S) DI SCHARGE MONITOR IN G REPORT (D MR) MAJOR (SU B R 01} Form Approved. OMB No. 204 0-0004 Ad dr ess_P ...Q.Jt O X 2 000 ___________ _ ______ _ 4-M 5.;..8 o_E_R--111 01 scHARG: I F O R ouTF ALL 1 01 ____ _ _________ _ A TT N: st e phan ie A. Ho w ard I r%: T!RING E FFLU EN T Fro m I I 01 ] To lL .. N O DI SCHAR G E NOTE: Read in structions before completing this form. PARAMETE R X QUANTITY OR LOADING QUALITY OR CONCENTRAnON NO. F R EQUENCY SAMPLE EX O F TYPE AVERAGE MAXI MUM UN ITS M I NIMU M AV E R A G E MAX IM UM UNITS ANALYS I S IC25 S T ATRE 7 DAY CHR SAMPLE ******** ******** Mo ni t orin g ******** ******** -23 CERIODAPHNIA MEASUREMENT No t Requ i red T R P38 1 0 PERMIT *******"*

                • -43.2 *-** ******** PERCENT SE MI COM P O S EFFLUEN T GROSS REQUIREMENT MINIMUM ANN U AL I C25 S T ATRE 7 DA Y C H R SAMPLE ******** ******** Mo ni to rin g ******** ******** .. 23 P JM EP H ALES MEASUREMENT No t Requ ir ed !T RP6C 1 0 PERMIT .........
  • ... ***** *-* 43.2 ******** ******** PERCENT SE MI C OM POS EFFLUEN T GROSS REQUIREMENT MIMINUM AN N UA L SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMP L E MEASUREMENT PERMIT REQU I REMENT SAMPLE MEAS U REMENT PERMIT REQUIREMENT SAMPLE MEASUREMEN T PERMIT REQUIREMEN T A NAME/TlTL E PRINCIPAL EX E C U TIVE OFFI C ER I Certify under penalty of law lha l llu document and all a ttachments were prepared Lnder my r'l TE L E PH O NE D A TE direction Of StJpetVosion on aa:ordance woth a syslem de s igned t o a ssure tha t quafofoed personnel M i chae l D. Skaggs properly gather and evaluate lhe information s ubmitt ed. Based on my a !he person or persons who manage the syslem. or lllose persons cforedly responsible rex galhenng the ncnnatoon.

the onformabon Stbnitl ed .s . to the best of my knowledg e and belief , true , accurate , Sequoyah S i te V i ce Pres i dent and complete I am aware lhat there are sognilicanl penalti es r ex soomottw>g la l s e informabon, oncluding the ol fone and ompn.sonmenl lor knowong v iolations TYPED OR P RINn D COMMENTS AND EXPlANATION OF ANY (Refer e nce ill/ iittilchments here Toxic i ty was not sampled i n Ap ril 2011. EPA Fonn 33 20-1 (R E V 3199) Previous edition s m a y be used Sequoya h S i te Vice P res i den t SIGNA TU RE OF P R I N CI PA L E X E C UT IV E OFFI CER O R AUTH O R IZ ED AG E NT 42 3 843-7001 11 05 09 I NU M BE R YEAR MO D AY P age 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) _&ldr§S_P...Q .Ji OX __________ _ _________ _ NATIONAl POLLUTANT DISCHARGE EU M INATIO N SYSTEM (NPDES) DISCHARGE MONITOR I NG REPORT (DMRJ MAJOR (SUBR01) EFFL U ENT Form ApP roved. OMS N o. 204()-0004 ATTN: stephanie A. Howard I t:!T r iNG e;mt ...... NO DISCHARGE D ... NOTE: Read in s tructions before completing this forrrL PARAMETER X OUANTfiT OR LOADIN G OUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAX I MUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE -*"** ******** 7 ............ 8 0 1 5/30 GRAB -12 MEASUREMENT 00400 1 0 PERMIT *****--**

                • .. 6 ........ 9 su THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE **"****** ******** ******** 16 16 0 2/30 GRAB .. 19 MEAS UREM ENT 00530 1 0 PERMIT ******** .......... ... *'*******

30 100 MGIL lWICEI GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH OIL AND GREASE SAMPLE ******** ******** **"*****'* <6 <6 0 2/30 GRAB -19 MEASUREMENT 00556 1 0 PERMIT ******** *****..-.:

    • -******** 15 20 MGIL lWICEI GRAB EFFLUENT GROSS REQU I REMENT MOAVG DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 0.853 1.540 ***'*****
  • "*******
                • 0 30/30 RCORDR 03 .. TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD ******tr:*

.........

                • .. SEE RCORDR EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

..... /' NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify IJI"ode< penally of law lhallln doc:unent and an attaclvnents were prepared IJI"ode< my ra TELEPHONE DATE direchon or supennsion 1n accordance with a syseem OOs;gned to assue lhat qualified persornel M ichael D. Skaggs property gather and evaluate the infOfTilalion submllled. Based on my i nq<.Wy of the per5IX1 or persons who manage the syseem, or lhose persons diredly responsible ror gau-ong the Sequoy ah Site Vice President 1nforma1Jon. the lflformabon submitted IS. 10 the best of my knowledge and belief, IIUe, accurate, 423 843-7001 11 05 09 Sequoyah S i te Vice President jand complete. I am aware rhat there are sogri6canl penai!Jes lor stbmotbng false information, SIGNATURE OF PRINCIPAL E XE CUTIVE I includ1ng the possibility of flOe and impnsonment lor knowing violations OFFICER OR AUTHORIZED ACE NT AREA I NUMBER YEAR MO DAY ITPED OR PRINTED rnnF COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all Jttachments here EPA Form 3320*1 (REV 3/99) Previous ed"mons may be used Page 1 of 1 PERMmEE NAM E/AD DRESS (Include Faa1rtv Name/location if D i fferent] NATIONAL POLLUTAm DISCHARGE arMINATION SYSTEM (NPDES} DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F onn APProved. OMB No. 2040-0004 __________ _ DISCHARGE 1 I coouNG wATER ____ _ AITN: stephanie A. Howard PARAMETER X QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM TEMPERATURE , WATER DEG. SAMPLE ****"****

                • .............

"* CENTIGRADE MEASUREMENT 00010 1 0 PERMIT *******"'*

            • "* -******** EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE, WAT ER DEG. SAMPLE "**"*"***** *****"***
    • 111:*****

... CENTIGRADE MEASUREMENT 00010 z 0 PERMIT ..........

              • '*

... ******** INSTREAM MONITORING REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & SAMPLE *****""*" **"***** ******** ... UPSTRM DEG.C MEASUREMENT 00016 1 0 PERMIT ******** ******** ... ******** EFFLUENT GROSS VALUE REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE ******** *******"* 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** EFFLUENT GROSS VALUE REQUIREMENT DAILY MX CHLORINE , TOTAL RESIDUAL SAMPLE ............ ******** ******** .. MEASUREMENT 50060 1 0 PERMIT ******** ******** ... *******"* EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE-C , RATE OF SAMPLE *"***'**'*"*

                • 04 CHANGE MEASUREMENT 82234 1 0 PERMIT '*******"*

2 DEGC ******** EFFLUENT GROSS VALUE REQUIREMENT DAILY MX SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penally of law thai this document and all atlachmenls were prepared tnder my .rectoon or superviSIOn in accordance wilh a system designed to assu-e thai qual-.r.ed personnel M ich ael D. Skaggs property galller and evaluate the Jnf"""' tian submitt ed. Based on my inquiry of the person or persons who manage the system. or IOO;e persans drectly responsible for galhe<ing the Sequoyah Site Vice President the nonnation submitted is. t o the best of my knawfe<lge and behef. true. accurate. complete. I am aware thallhere are signJfJCa nt penalties for s ubmiUJng false inlonnation. mcluding the possib1h1y ol fine and Impn>onment for knowing v iolations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachm e nts herE No D ischa rge this Pe riod EPA Form 3320-1 (REV 3199) Previous editions may be used EFFLUENT ... NO DISCHARGE I XX I ... NOTE: Read inslructions be fore completinQ this form QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS ***'*"**** 04 .........

    • REPORT DEGC CON TIN CALC TO DAILYMX uous *******'*

04 ******** 30.5 DEGC CONTIN CALCTD DAILYMX uous ******** 04 ......... 5 DEGC CONTIN CALCTD DAILY MX uous *"******"*

                • -******** *"*"****"*"*

.. CONTIN RCORDR uous 19 0.1 0.1 MGIL F i ve per CALC TO MOAVG DAILY MX Week ******** *"***"** .. ******** *******-* .,. CONTIN CALC TO uous DATE 423 843-7001 05 11 09 SIGNATURE OF PRINCIPAL EXECUnVE I OFFICER OR AUTHORIZED ACE NT NUMBER YEAR MO DAY Page 1 of 1 P E RMITTEE NAM E/A DDRESS (Incl ud e FaalitV N ame/location if D iffere nt) Address_p....Q .Jl0X.2..QQO ___________ _ ___ _ NA TIONAL POLLliT ANT DISCHARG E E LI M I NA TIO N SYS TEM (N P DE S} DISCHARGE MONITORING REPORT (D M R} MAJOR (SUBR 01) EFFLUENT Form ApProved. OMB N o. 204G-0004 ATIN: stephanie A. Howard MQ 04 NO DISCHARGE I XX I ... NOTE: Read instructions be for e complelin!llh is form. PARAMETER X OR LOADING OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNIT S ANALYSIS IC25 STATRE 7DAY CHR SAMPLE *****-*** ***'***** .......... .......... ... 23 CERIODAPHNIA MEASUREMEN T TRP38 1 0 0 PERMIT ******** ******** -** 43.2 ******"** ...........

  • PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC25 STATRE 70AY CHR SAMPLE ***'*'**** ******** **-*"'***** **'****** .. 23 PIMEPHALES M EA SUREMENT [TRP6C 1 0 0 PERMIT ******** ******** ...... 43.2 *"*"**"****

..... _ PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL SAMPLE MEA SU REMENT PERMIT REQUIREMENT SAMPLE MEASU REM ENT PERMIT REQUIREMENT SAMPLE MEA S UREMEN T PERMIT REQU I REMENT SAMP L E MEASUREME NT PERMIT REQUIREMENT SAMPLE MEAS U REMENT PERMIT REQU I REMENT NAME/TlTLE PRINCIPAL EXECUTIVE OFFICER I Cer1Jfy LRier penalty of law tha t U.S documeol and aD a ttachments were prepared o.n1er my M i chael D. Skaggs uorec:IJon or supeMSJOn '" acccrdance With a system designed t o ass.re thai qua l ified petsorne1 propelly gather and evalua:e the infonnallon SUbmitt ed Basad 00 my inquiry or lhe person or persons wt1o manage the system, or those persons diredly responsible lor ga1herwlg the information. the informa t ion subnitled os

  • to the be st or my knowledge and belief. true, acaJrate, Sequoyah S i te V i ce P r es i dent and complete I am aware tha t lhere ace signtrocanl penaltJes lor slbmitl.ng false in f ormation, onc:lu<Mg the possibility of fone and lor knDwong 1110labons OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS f R e ference all attachments here No Discharge th i s Per i od EPA Form 3320-1 (REV 3199) Previous editions may be used I TELEPHONE DATE 423 843-700 1 11 05 09 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY r-.nnF Page 1 o f 1 PERMITIEE NAME/ADDRESS (Include Faci li ty Name/L ocation if D i ffere n l) . ..§..OX 2..Q9Q_ __________ _

___ _ NAT I ONAl POLL UT ANT DISCHARGE ELIM I NATI O N SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: stephanie A. Howard From: I 01 To :1 :1:1: ...... ... _:-_ ... .... NO DISCHARGE I XX I NOTE: Read i nstructions befo r e comPielill!llhis form. PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE ******** ******** ******** ................ MEASUREMENT ... 19 00300 1 0 PERMIT ******** ******** -** 2 "****** *****'*** MG/l TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ****"****

              • "*
                • '*'******'*

.. 19 MEASUREMENT 0 0530 1 0 PERMIT *******'* ******** ..... ..........

  • .........
    • 100 MGJL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE ***"*****

.................

                • " ******** ... 25 MEASUREMENT 00545 1 0 PERMIT ******** ******** **-******** *******'*

1 ML/l ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX MONTH FLOW , IN CONDUIT OR THRU SAMPLE ........... ******** ******** 03 .. TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ******** ***** .... * ******** . ONCE/ ESTlMA EFFLUENT GROSS REQUIREMENT MOAVG DAILY MX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQU I REMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEITlTLE PRINCIPAL EXECUTIVE OF F ICER I Certify ooder penally of l aw that th i s doc:umenl ani al a ttactmen ts we<e prepared tn1er my TELEPHONE DATE direc:bon << supeiVlSOOO W1 accordance with a syst e m designed to assur e tha i quaf lfied personrel M i chael D. Skaggs property gather and eva luat e the information submitt ed. Based on my inQwy of the person<< q oyah Site pe rsons who manage the sy stem , or those persons diredly re sponsibl e lor ga thering the inlormatJoro , the 111 l onna t ioo submi tted i s , t o the best o f my """'"edge and be lte f , true , acx:ur at e. 423 843-7001 1 1 05 09 Sequoyah Site Vice President and complet e I a m awar e that there a re signtficanl pen albes f or sl.brrotbng false inlormallorl. SIGNATURE OF PRINCIPAL EXECUTIVE I including the pos s ibi li ty or f on e and mprisonment for knowing violations OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMEN T S AND OF ANY VIOLAT I ONS (Reference all attachments here During this report i ng period , there has been no flow f rom the Dredge Pond other than that result i ng from rainfall. EPA Form 3320-1 (REV 3199) Pre viou s efi ibons may be usefi Page 1 of 1 REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-April2011 DMR ORGANIZATION

Environmental DOCUMENT PREPARED BY: Brad Love DATE: 05/09/2011 CONCURRENCES Name R c Signature

-Comment Date v N B.M. Love X S. A. Howard X D.A. Day X M.D. Skaggs X INSTRUCTIONS

Originator will determine the rev i ew/concurrence assignment.

REVIEW: Examine technical content and commitments made. A rev i ew (RV) should confirm the truth and accuracy of factual statements and indic ate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose , logical in construction , and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. S58 110608 800-NPDES CORRESPONDENCE June 8 , 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Contro l Enforcement & Compliance Section 6 1 h Floor , L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR MAY 2011 Enclosed is the May 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection at the Diffuser Pond Inlet of the Yard Pond effluent due to a transformer oil sp ill that reached the Yard Drainage Pond has been discontinued as of 5/14/2011. Samples collected from 9/23/2010 through 5/14/2011 have all yielded result s below detection l imits for oil and grease. If you have any questions or need additional information , please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Env ironm ental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supe!Vision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the inform ation submitted. Based on my inquiry of the person or persons who manage the system , or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information , including the possibility of fine and imprisonment for knowing violations. Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Bu ilding, Suite 550 540 McCa l lie Avenue Chattanooga , Tennessee 37402-2013 B. E. Brickhouse , LP 5U-C G. M. Cook , OPS 4A-SQN D. A Day , POB 2A-SQN S. A Howard , OPS 5N-SQN K. La ng do n , POB 2B-SQN U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington , DC 20555 D. B. Nida, LP SU-C A A Ray, WT 11A-K G. R. Signer , WT 6A-K M. D. Skaggs , OPS 4A-SQN K. M. Hodges (EDMS), LP 2V-C P E R MITT EE NAME/ADDR ES S (Inclu d e Facility N a meA.oca l ion if Different) NA TIO N AL P OLLUTANT DISCHARGE EU M l N A TI O N S Y STEM (N P D E S) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F onn A pproved. .&IQ[!ID J...Q.JtDX 2.QOO_ __________ _ ___ _____ --4...;..M 5..;;..s 0;..e_R_--t,l DISCHARce 1 I DISCHARGE _________ _ EFFLUENT I YEAR I MO 05 ... ,. NO DISCHARGE ATIN: stephanie A. Howard NOTE: Read i nstructions before complelin!l lh is l oon PARAMETER X QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE ex OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALY SIS TEMPERATURE , WATER DEG. SAMPLE .............. ****"**** -... ....... 35.7 0 31 I 31 RCORDR -04 CENTIGRADE MEAS U REMENT 00010 1 0 PERMIT ******** *'***"****

        • ******"**

........... Req. Mon. DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPL E *******""' ******** ******** ******** 25.9 0 31 I 31 MODELO ... 04 CENTIGRADE MEASUREMEN T 00010 z 0 PERM IT ******** ******** --******** **"**** 30.5 DEG.C. CONTI CALC TO INSTREAM MONITORING REQUIREMENT DAILYMX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE _ ..............

                • ****'****
                • 2 0 31/31 CALC TO -04 UPSTRM DEG.C MEASUREMEN T 00016 1 s PERMIT *'*******
                • -** ******** ******** 3 DEG.C. CONTI CALCTD EFFLUENT GROSS REQ U IREMENT DAILY MX NUOUS FLOW , IN CONDUIT OR THRU SAMP LE ******** 1644 *"*******
        • "**** **"*-0 31 I 31 RCORDR 03 .. TREATMENT PLANT MEASUREMENT 50050 1 0 PERM IT .............

Req. Mon. MGD ******"* ........... ... .......... ..... CONTI RCORDR EFFLUENT GROSS REQ U IREMENT DAILY MAX NUOUS CHLORINE , TOTAL RESIDUAL SAMPLE ******** ******** ******** 0.023 0.046 0 21 I 31 GRAB .. 19 MEASUR EM E NT 50060 1 0 PERMIT *****'*** ******** --******** 0.1 0.1 MGJL FIVE PER CALCTD EFFLUENT GROSS REQUIREMENT MOAVO DAILY MAX WEEK TEMPERATURE -C , RATE OF SAMPLE ............... 0 ******** ******** 0 31 I 31 CALCTO 62 ** CHANGE MEASUREMENT 8 2 234 1 0 PERMIT *******"* 2 DEG ******** .........

              • .... CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILYMX CfHR NUOUS SAM PLE MEASU R EMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify Wlder penally of law lhallhis doCI.mem and al attachments were prepared Wlder my Pre*<eol TELEPHONE DATE Michael D. Skaggs ...;. ection or supennsion in accordance With a system designed to assure that qualified personnel properly galhef and evaluate lhe onfonnation s.bn*lled.

Based on my Wlquiry ollhe person or persons who manage lhe system. or those persons directly responsible f or gathering lhe irtormai!Dn, lhe onformation S<.tlmilled IS

  • l O lhe best of my knowledge and belief , true. aca.r.Jte , 423 843-7001 11 06 07 Sequoyah S i te Vice Pres i dent and complete I am aware that !here are s.gnlf>eant penalties for st.t>mitbng false infonnatior\

SIGNATURE OF PRINCIPAL EXECUTIVE I *ncluding lhe possibility of fine and irnprisom>enl for knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS /R e fer e nc e all attael7m e nts her e No dosed mode operation. Veliger monitoring data is induded as an attachment. The following inject i ons occurred: 1. Towerbrom 960 2. Floguard MS6236 (ma x. calc. cone. was 0.11 mg/L-I i mit 0.2 mg/L) 3. Spectrus CT1300 (max. ca l c. con e. was 0.039mg/L-Iimit 0.050mg/L) EPA Form 3320*1 (REV 3199) Previou s ed itions may b e used Page 1 of 1 Mean# of Water Mean# of Water SUB NOTES:% Sample Date ZM/m3 %Settlers Temp.fC) Sample Date Asiatic Temp.fC) LOCATION LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-ISV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB 02/08/2011 0 0 9 02/08/2011 0 9 1-25-545 MJW 02/15/2011 0 0 23 02/15/2011 0 23 1-25-545 MLW 02/22/2011 20 100 10 02/22/2011 0 10 1-25-545 PB 03/01/2011 0 0 11 03/01/2011 0 11 1-ISV-24-1236 PB 03/08/2011 0 0 11 03/08/2011 0 11 1-ISV-24-1236 WE 03/16/2011 22 0 11 03/16/2011 0 11 1-ISV-24-1234 MLW 03/23/2011 0 0 11 03/23/2011 0 11 1-ISV-24-1234 MLW 03/30/2011 0 0 12 03/30/2011 0 12 1-15v-24-1236 MLW 04/06/2011 18 100 15 04/06/2011 0 15 1-ISV-24-1234 HMW 04/08/2011 45 100 15.5 04/08/2011 0 15.5 1-1SV-24-1236 WAW/PB 04/20/2011 21 100 16 04/20/2011 0 16 1-1SV-24-1236 PB May 2011 No Samples Collected PERMITTEE NAME/ADDRESS (Include Facifity Name/locat io n if Dffferentl Addre ss _p....Q.Jl O X 2000 ___________ _ ___ _(INTER O F FIC E OPS*SN*SQN! ______ _ NATIONALPOLLUTANT D ISCHARGE ELIM I NATION SYSTEM (NPDES I DISC H A.R GE MONI T O R ING REPOR T (DMRJ T N 0 0 2 64 5 0 101 T MAJOR (SUBR 01) F-FI N AL Fonn App r oved. OMB No. 2040-0004 _______ _ ERM I T N U M BE D I SCHAR G E NUMBE BIOMONITORING F O R OUTFA L L 101 EFFLU E NT A TT N: step ha nie A. Howard I Y EA R I M O M Q 05 [ 0 1 ] To r 11 I 05 QA Y 31 NO D ISCHARGE D ...... NO TE: Read instructions before th i s form. P ARA M ETER X QU ANT ITY OR LOADING OUAUTY OR CO N CENTR.AT I O N NO. FR E QUENCY S A M PLE E X OF AVERAGE M AXIM U M UNITS MINIMUM AVERAG E MAX I MUM UNITS ANAlYSIS IC25 STATRE 7 DAY CHR SAMPLE ******"**

                • >100.0 ******** ******** 0 1/180 COMPOS -23 CERIODAPH NI A MEASUREMENT TR P3 8 1 0 PERMIT ****:I:***
                • --43.2 ******** **'******

P ER CE NT S E MI COMPOS E FFL UENT G R OSS R E QUIREM E NT M I N I MUM ANNUAL I C25 STATRE 7DA Y CH R SAMPLE ******** ******** >1 00.0 ******** ******** 0 1/1 80 COMPOS -2 3 PIM EPHALES MEASUREMENT TRP6C 1 0 PERMIT ******** ******** -43.2 *****'*"*'*

            • '**

P ER C E N T S E MI COMPOS EFFLUEN T GROSS REQUIREMENT MIM INU M ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMrT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIR E MENT SAMPLE MEASUREMENT PERMIT REQUIREM E NT NAME/TlnE PRINCIPAL E X E C U TIVE O F FIC E R I Certify ooder penalty ollaw lhat this documenl and aN attachments were prepared under my Q,?I:W re,, . .,, T ELE PHON E DATE direction or s....,e<vision in aoconlance Yoith a system designed lo asS\Jre lhat qualified personne l Michael D. Skaggs properly gather and evaluate lhe infonnat ion submitted. Based on my inqtjry or the person or pe1SOOS who manage lhe system, or lhose persons diredJy responsible for galhering lhe 423 843-7001 11 06 07 i nlonnalion , lhe information Slbnitted is

  • t o lhe best of my l<nowledge and belief , true. accurate , Seq uo yah Site Vice Pr e side n t and complete.

I am a"N are lhat there are Signi!icari penalties for sOOmilling false information, including the possibility or f1<1e and ror knowing violations. SIGNATUR E OF PRINCI P AL EXECunV E I OR PR I NT E D COMM E NTS AN D EXP LA NATION OF ANY VIOLATIONS (ReFerence all attachments here Tox i city sampling began o n May 1 and ended on May 6. The toxicity report is induded. EP A Fonn 3 3 2 0-1 (R EV 3199) Previous editions may be used O F F IC ER OR ALITH O R IZ E D AGENT NUM BE R Y E AR MO DAY Page 1 o f 1 June 3, 2011 Bradley M. OPS 5N-SQN SEQUOY AH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, MAY, 2011 Per your request, I have also submitted an electronic copy of the subject report. Outfall J 0 I samples collected May 01-06, 2011, showed no toxic effects to fathead minnows or daphnids. The resulting IC2s values for both species were> I 00 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfaii 1 01 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mmtality occurred in minnows exposed to treated and UV -treated samples. Please call me at your convenience if you have any questions or comments following your review of the report. Donald W. Sno grass Manager (Acting), Environmental Engineering Field Team-Muscle Shoals CTR2L-M Attachment Cc (Attachment): R.M. Sherrard, PSC IX-C (Electronic copy) EDMS, MPB IE -M (Electronic copy) TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: June 03,2011 1. Facility I Discharger: Seguoyah Nuclear Plant I TV A 2. County I State: Hamilton I Tennessee

3. NPDES Permit#: TN0026450
4. Type of Facility:

Nuclear-Fueled Electric Generating Plant 5. Design Flow (MGD): 1.579 6. Receiving Stream: Tennessee River (TRM 483.6) 7. 1Q10: 3.491 8. Outfall Tested: 101 9. Dates Sampled: May 01 -06, 2011 10. Average Flow on Days Sampled (MGD): 1635.7, 1623.12, 1618.58 11. Pertinent Site Conditions: Production I operation data will be provided upon request. 12. Test Dates: May 03-10,2011 13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia) 15. Concentrations Tested(%): Outfall101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100.0 Pimephales promelas: UV treated Outfall101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100.0 16. Permit Limit Endpoint(%): Outfall101: IC22. = 43.2% 17. Test Results: Outfall 101: Pimephales promelas: Kas > 100% Ceriodaphnia dubia: Kas > I 00% UV treated Outfall 1 01 : Pimephales pro mel as: Kz 5 > 1 00% Page 1 of 100

18. Facility Contact: Brad Love Phone#: (423) 843-6714 19. Consulting I Testing Lab: Environmental Testing Solutions.

Inc. 20. Lab Contact: Jim Sumner Phone#: (828) 350-9364 21. TV A Contact: Donald W. Snodgrass Phone#: (256) 386-2787 22. Notes: Outfall101 samples collected May 01-06, 2011, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 2 of 100 METHODS

SUMMARY

Samples: 1. Sampling Point: Outfall I 01, Intake 2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (oC) (mg/L) LastUsedBv 05-01-11 0800 to 05-02-11 1512 o.7, o.8t <0.10 05-03-11 1415 101 05-02-11 0700 05-04-11 1318 05-01-11 0845 to 05-02-11 1512 1.2 <0.10 05-03-11 1415 Intake 05-02-11 07 45 05-04-11 1318 05-03-11 0800 to 05-04-11 1437 0.7, 0.9t <0.10 05-05-11 1319 101 05-04-11 0700 05-06-11 1317 Intake 05-03-11 0830 to 05-04-11 1437 0.8 <0.10 05-05-11 1319 05-04-11 0730 05-06-11 1317 05-05-11 0800 to 0.9, 0.9, 05-07-11 1319 101 05-06-11 0700 05-06-11 1515 1.5tt <0.10 05-08-11 1322 05-09-11 1316 05-05-11 0830 to 05-07-11 1319 Intake 05-06-11 1515 0.9 <0.10 05-08-11 1322 05-06-11 0730 05-09-11 1316 *TRC =Total Residual Chlorine tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival. ttsamples were collected in two 2.5 gallon cubitainers and one 5 gallon cubitainer. Temperature was measured in each cubitainer upon arrival. 4. Sample Manipulation: Samples from Outfalll01 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath. Page 3 of 100 Aliguots ofOutfalliOI and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics. Inc.) for 2 minutes. Pimephales promelas Test Organisms:

1. Source: Aquatox, Inc. 2. Age: 21.75-22.25 hours old Test Method Summary: 1. Test Conditions:

Static, Renewal 2. Test Duration:

3. Control I Dilution Water: Moderately Hard Synthetic
4. Number of Replicates: 5. Organisms per Replicate:

10 6. Test Initiation: (Date/Time) Outfall101 05-03-11 1415 ET UV Treated Outfall 101 05-03-11 1345 ET 7. Test Termination: (Date/Time) Outfall101 05-10-11 1325 ET UV Treated Outfall 1 01 05-10-11 1342 ET 8. Test Temperature: Outfall101: Mean = 24. 7°C (24.2-25.2°C} Ceriodaphnia dubia In-house Cultures < 24-hours old Static, Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic 1 05-03-11 1315 ET 05-10-11 1224 ET Mean= 25.0°C (24.7-25.2°C} Test Temperature: UV-Treated Outfall101: Mean = 24.8°C (24.2-25.2°C} 9. Physical I Chemical Measurements: Alkalinity, hardness. total residual chlorine, and conductivity were measured at the laboratory in each 1 00% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and exposure test solutions were analyzed daily for pH and dissolved oxygen. 10. Statistics: Page 4 of 100 Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville, CA}. TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1. Results of a Pimephales promelas Chronic/7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 03-10, 2011 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used -days) (%Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 98 98 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 98 95 93 Test Solutions Mean Dry Weight (mg) (%Effluent) (replicate number) 1 2 3 4 Mean Control 0.704 0.756 0.799 0.819 0.770 10.8% 0.849 0.889 0.898 0.768 0.851 21.6% 0.659 0.886 0.913 0.879 0.834 43.2% 0.761 0.844 0.841 0.853 0.825 86.4% 0.845 0.805 0.716 0.797 0.791 100.0% 0.801 0.696 0.863 0.800 0.790 Intake 0.893 0.794 0.774 0.660 0.780 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.2% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 1 OOILC 5 o: TUc = 100/ IC2s Page 5 of 100 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 03-10,2011 using effluent from Outfall101. Percent Surviving Test (time interval used-d<!Y_s) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 28 28 31 31 31 31 30 29 29 29 29.7 10.8% 33 30 34 32 27 33 30 28 30 32 30.9 21.6% 33 32 31 36 29 32 31 31 31 33 31.9 43.2% 36 32 34 30 35 36 35 34 32 34 33.8 86.4% 34 35 33 35 36 37 36 35 33 35 34.9 100.0% 38 36 36 37 35 38 36 36 36 36 36.4 ICz 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.2% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 1 00/LCso: TUc = 100/ ICzs Page 6 of 100 TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 03-10,2011 using water from Intake Percent Surviving Test (time interval used -days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effluent) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 26 34 30 29 27 30 32 31 29 29.7 Intake 37 37 35 35 37 35 32 36 38 36 35.8 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/ A Permit Limit: N/ A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa = 1 00/LCso: TUc = 100/ IC2s Page 7 of 100 TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets) 3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 03-10, 2011 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used -days) (%Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 98 98 98 86.4% 100 100 100 100 98 98 98 100.0% 100 100 98 98 98 98 98 Intake 100 100 100 100 100 100 98 Test Solutions Mean Dry Weight (mg) replicate number) (%Effluent) 1 2 3 4 Mean Control 0.714 0.908 0.853 0.849 0.831 10.8% 0.901 0.813 0.867 0.888 0.867 21.6% 0.896 0.793 0.779 0.829 0.824 43.2% 0.879 0.852 0.847 0.773 0.838 86.4% 0.850 0.770 0.774 0.797 0.798 100.0% 0.816 0.802 0.660 0.840 0.780 Intake 0.948 0.883 0.925 0.799 0.889 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA *TUa= 100/LCso: TUc= 100/IC2s REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) Species Date Time Duration Toxicant Results CIC2J Pimephales promelas May 03-10,2011 1445 7-days KCl 0.78 giL Ceriodaphnia dubia May 03-10,2011 1255 7-days NaCl 1.07 giL Page 8 of 100 PHYSICAUCHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges fur Pimephales promelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outmn 101 perfunred May03-10, 2011. Test Sample ID Temperature ("C) Initial Control 24.8 24.7 -24.9 10.8% 24.9 1 24.7 -25.0 21.6% 24.9 24.7 -25.0 1:1, 24.9 43.2% '::1 24.7 -25.0 24.9 s.. 86.4% .§ 24.7 -25.1 25.0 100.0% 24.9 -25.2 Intake 24.9 24.7 -25.1 Control 24.9 24.8 -24.9 10.8% 25.0 24.9 -25.0 .!;! 25.0 -c:. 21.6% .§ 24.9 -25.0 .!;! 25.0 1:: 43.2% §-24.9 -25.0 25.0 86.4% 24.9 -25.1 100.0% 25.1 24.9 -25.2 Intake 24.9 24.9 -25.1 Overall temperature ("C) Pimephales promelas Ceriodaphnia dubia Page 9 of 100 Flnal 24.6 24.4 -24.8 24.6 24.2 -24.8 24.6 24.4 -24.8 24.6 24.5 -24.8 24.5 24.4 -24.6 24.5 24.3 -24.7 24.4 24.3 -24.6 25.0 24.7 -25.2 25.0 24.8 -25.2 25.0 24.8 -25.2 25.0 24.8 -25.1 25.0 24.8 -25.2 25.0 24.8 -25.2 24.9 24.8 -25.2 Average 24.7 25.0 Dissolved Oxygen (mg/L) pH(S.U.) Initial Flnal Initial F1nal 7.6 7.7 7.81 7.77 7.4 -7.7 7.5 -7.9 7.73 -7.89 7.68 -7.86 7.8 7.7 7.85 7.73 7.5 -8.2 7.3 -8.0 7.80 -7.93 7.64 -7.87 7.9 7.7 7.85 7.73 7.7 -8.1 7.3 -8.0 7.79 -7.93 7.64 -7.86 7.8 7.7 7.83 7.73 7.7 -8.1 7.4 -8.1 7.77 -7.92 7.66 -7.86 7.8 7.7 7.80 7.71 7.7 -8.0 7.4 -8.1 7.72 -7.92 7.66 -7.85 7.9 7.7 7.79 7.71 7.7 -8.1 7.4 -8.0 7.71 -7.90 7.64 -7.85 7.9 7.7 7.78 7.70 7.8 -8.1 7.3 -8.0 7.69 -7.92 7.64 -7.87 7.6 7.7 7.81 7.84 7.4 -7.7 7.4 -8.0 7.73 -7.89 7.77 -7.90 7.8 7.7 7.85 7.82 7.5 -8.2 7.5 -8.0 7.80 -7.93 7.71 -7.90 7.9 7.8 7.85 7.84 7.7 -8.1 7.6 -8.0 7.79 -7.93 7.77 -7.91 7.8 7.8 7.83 7.84 7.7 -8.1 7.6 -8.0 7.77 -7.92 7.76 -7.90 7.8 7.8 7.80 7.84 7.7 -8.0 7.6 -8.0 7.72 -7.92 7.74 -7.93 7.9 7.8 7.79 7.83 7.7 -8.1 7.5 -8.0 7.71 -7.90 7.72 -7.93 7.9 7.8 7.78 7.82 7.8 -8.1 7.6 -8.1 7.69 -7.92 7.71 -7.91 Minimum Maximum 24.2 25.2 24.7 25.2 Conductance Alkalinity Hardness Total Residual (Jlmhos/cm) (mg/L CaC0 3) (mg/L CaC03) Chlorine (mg/L) 321 59 90 305 -333 58 -59 89 -91 299 283 -311 282 272 -292 249 241 -257 188 179 -207 163 58 63 <0.10 157 -174 54 -61 55 -71 <0.10 -<0.10 164 59 62 <0.10 155 -178 55 -61 55 -67 <0.10 -<0.10 321 59 90 305 -333 58 -59 89 -91 299 283 -311 282 272 -292 249 241 -257 188 179 -207 163 58 63 < 0.10 157 -174 54 -61 55 -71 <0.10 -<0.10 164 59 62 <0.10 155 -178 55 -61 55 -67 <0.10 -<0.10 PHYSICAUCHEMICAL

SUMMARY

Water Chemistry Mean Vahres and Ranges fur Pimephales promelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outmll 101 perfunned May 03-10, 2011. Test Sample ID Temperature \C) Initial Control 24.9 24.8 -24.9 10.8% 24.9 .i 24.9 -25.0 21.6% 25.0 24.9 -25.1 l:l. 25.0 J! 43.2% '::1 24.9 -25.1 ..::: 25.0 86.4% .§ 24.9 -25.1 =... 25.1 100.0% 24.9 -25.2 Intake 25.0 24.8 -25.1 OveraU temperature \C) Pimephales promelas Page 10 of 100 Final 24.6 24.4 -24.9 24.6 24.3 -24.8 24.6 24.2 -24.8 24.6 24.5 -24.7 24.6 24.5 -24.7 24.6 24.5 -24.7 24.6 24.4 -24.7 Average 24.8 Dissolved Oxygen (mg/L) Initial Final 7.8 7.7 7.5 -8.1 7.5 -8.0 7.8 7.7 7.7 -8.1 7.3 -8.0 7.8 7.7 7.7 -8.1 7.3 -7.9 7.9 7.6 7.7 -8.1 7.3 -7.9 7.9 7.7 7.8 -8.1 7.4 -8.0 8.0 7.7 7.9 -8.1 7.4 -7.9 8.0 7.8 7.9 -8.2 7.5 -8.0 Minimum Maximum 24.2 25.2 pH(S.U.) Conductance Alkalinity Hardness Initial Final (Jlmhos/cm) (mg/L CaC0 3) (mg!L CaC0 3) 7.83 7.74 311 59 92 7.78 -7.91 7.66 -7.84 299 -326 57 -60 90 -96 7.85 7.75 297 7.79 -7.92 7.66 -7.85 286 -310 7.86 7.74 283 7.80 -7.93 7.66 -7.86 270 -293 7.85 7.73 250 7.79 -7.91 7.65 -7.86 240 -256 7.83 7.71 187 7.75 -7.90 7.62 -7.87 178 -197 7.81 7.69 164 57 69 7.73 -7.91 7.62 -7.86 154 -178 52 -61 63 -76 7.79 7.70 164 58 69 7.71 -7.90 7.63 -7.85 155 -175 52 -63 63 -76 Total Residual Chlorine (mg/L) <0.10 <0.10 -<0.10 <0.10 <0.10 -<0.10

SUMMARY

I CONCLUSIONS OutfalllOI samples collected May 01-06,2011, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall I 01 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 11 of 100 Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment.

Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution. DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL 1. Pimephales promelas None 2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None 2. Ceriodaphnia dubia None Page 12 of 100 PHYSICAL AND CHEMICAL METHODS 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
2. Instruments:

All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B. 4. Dissolved oxygen was measured by SM 4500 0 G. 5. The pH was measured by SM 4500 H+ B. 6. Conductance was measured by SM 2510 B. 7. Alkalinity was measured by SM 2320 B. 8. Total hardness was measured by SM 2340 C. 9. Total residual chlorine was measured by ORION Electrode Method 97-70. QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013.

Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC2 5 values in giL KCl or NaCl. 2. Standard Toxicant:

Potassium Chloride (KCl crystalline) for Pimephales promelas. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia. 3. Dilution Water Used: Moderately hard synthetic water. 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses. Page 13 of 100 REFERENCES

1. NPDES Permit No. TN0026450.
2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002). 3. Standard Methods for the Examination of Water and Wastewater, 21st Edition, 2005. 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 14 of 100 Page 15 of 100 Sequoyah Nuclear Plant Biomonitoring May 03-10,2011 AppendixB Diffuser Discharge Concentrations of Total Residual Chlonne, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998-May 6, 2011 Page 16 of 100 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, Date 08/26/2001 08/27/2001 08/28/2001 08/29/2001 08/30/2001 08/3112001 11/25/2001 11126/2001 11127/2001 11128/2001 11129/2001 11130/2001 12/09/2001 12/10/2001 12/1112001 12/12/2001 12/13/2001 12/14/2001 Page 17 of 100 March 12, 1998-May 6, 2011 PCL-401 mg!L Copolymer 0.009 0.028 0.009 0.009 0.009 0.009 0.009 0.019 0.019 0.018 0.019 0.019 0.019 0.019 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.021 0.021 0.021 0.020 0.021 0.020 0.02 0.019 0.019 0.02 0.02 0.02 0.02 0.02 Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, Date 01/02/2002 01103/2002 ' 01/04/2002 01105/2002 01106/2002 01/07 05/05/2002 05/06/2002 05/07/2002 05/08/2002 05/09/2002 05/10/2002 08/04/2002 08/05/2002 08/06/2002 08/07/2002 08/08/2002 Page 18 of 100 March 12, 1998-May 6, 2011 PCL-401 mg/L Copolymer 0.02 0.014 0.014 0.014 0.023 0.023 0.023 0.008 0.008 0.02 0.02 0.019 0.02 0.019 0.018 0.018 0.019 0.019 O.o18 O.o18 O.o18 O.o18 O.o18 O.ol8 0.019 0.020 0.020 0.020 0.009 0.021 0.021 0.021 0.021 0.022 Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth ofMicrobiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, Date 08/03/2003 08/04/2003 08/05/2003 08/06/2003 08/07/2003 08/08/2003 10/05/2003 10/06/2003 10/07/2003 10/08/2003 10/09/2003 10/10/2003 02/01/2004 02/02/2004

02/03/2004 02/04/2004 . 02/05/2004 02/06/2004 05/04/2004 05/05/2004 05/06/2004 05/07/2004 05/08/2004 05/09/2004 Page 19 of 100 March 12, 1998-May 6, 2011 PCL-401 mg/L Copolymer 0.020 0.014 0.014 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.020 0.022 0.024 0.009 0.009 0.009 0.009 0.009 0.009 0.019 0.014 0.013 0.020 0.021 0.020 0.019 0.020 0.020 0.019 0.019 0.020 Date 11/07/2004 11108/2004 11/09/2004 11/10/2004 11/11/2004 1111 02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/1112005 06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10/2005 07117/2005 07/18/2005

07/19/2005 07/20/2005 07/21/2005 07/22/2005 10/30/2005 10/3112005 1110112005 11102/2005 11103/2005 11104/2005 11114/2005 11115/2005 11116/2005 11117/2005 11118/2005 11119/2005 Table B-I. Sequoyah Nuclear Plant Diffuser (Outfall I 0 I) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, I998-May 6, 20II Page 20 of 1 00 Date 11112/2006 11113/2006 11114/2006 11115/2006 11116/2006 11117/2006 11126/2006 11/27/2006 11128/2006 11129/2006 11130/2006 12/0112006 05/28/2007 05/29/2007 05/30/2007 05/3112007 06/0112007 06/02/2007 12/02/2007 12/03/2007 12/04/2007 12/05/2007 12/06/2007 12/07/2007 04/13/2008 04/14/2008 04/15/2008 04/16/2008 04/17/2008

04/18/2008 10/26/2008 10/27/2008 10/28/2008 10/29/2008 10/30/2008 10/3112008 Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall I 0 I) Discharge Concentrations of Chemicals Used to Control Growth ofMicrobiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, I998-May 6, 20II Cuprostat-PF mg/L Azole Page 21 of 100 MSW 101 mg/L 0.015 O.Q15 O.o15 O.Q15 0.015 O.Q15 0.030 0.030 0.030 Date 02/08/2009 02/09/2009 02110/2009 02/11/2009 02/12/2009 02/13/2009 05/10/2009 05/11/2009 05/12/2009 05/13/2009 05/14/2009 05/15/2009 11 11 5/2009 11116/2009 11/17/2009 11/18/2009 11/1912009 11/20/2009 05/09/2010 05/10/2010 05/11/2010 05/12/2010 05113/2010 05/14/2010 Table B-1. Sequoyah Nuclear Plant Diffu ser (Outfall lO I) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 6, 20 I I Sodium Towerbrom PCL-PCL-401 CL-363 Cuprostat H-I 30M Nalco Spectrus H-150M Hypochlorite mg!L 222 mg/L mg/L -PF mg/L mg!L 73551 CT1300 mg/L mg/L TRC mg!L Copolymer DMAD Azole Quat mg!L mg/L Quat TRC Phosph EO/PO Quat ate -0.0197 -----0.017 ---0.0237 -----0.017 ---0.0104 -----0.021 ---0.0155 -----0.017 ---0.0106 -----0.017 -------------0.0129 ---------0.0415 -------0.0446 -0.0053 -------0.0396 -0.0049 -------0.0396 -<0.0141 -------0.0397 -<0.0160 ---------0.025 ---------0.0152 ---------0.0255 ---------0.0306 ---------0.0204 --------0.0093 ---------0.0192 ---------0.0055 ---------0.0100 ------0.039 --0.0171 ------0.039 --0.0041 -----0.039 --0.0099 ------0.039 -Page 22 of 100 MSW 101 mg/L Phosphate

D a te 10/31/2010 11/01/2010 11/02/2010 11/03/2010 11/04/2010 1 1/05/2010 05/01/2 011 05/02/2011 05/03/2011 05/0 4/20 11 05/05/20 11 05/06/2011 Table B-1. Sequoyah N uclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemica l s Used to Contro l M i crobiologica ll y Induced Corrosion Mollusks , During Toxicity Test Sampling, March 12 , 1998-May 6 , 2011 Soclium T owerbrom PCL-PCL-401 CL-363 Cupro st at H-130M N a l co Sp e ctrus H-150M Hypochlorite m g/L 222 m g/L mg/L -PF m g/L mg/L 73551 CT1300 m g/L mg/L TR C mg!L C opo l ymer DMAD Azo l e Quat mg/L mg/L Quat TRC Phospb EO/PO Quat ate -----------0.0 1 22 ---------O.Ql12 ---------0.01 6 3 ---------0.0107 ---------0.0132 --------------------------0.04 ---------0.04 --0.0155 ------0.04 --0.01 7 9 ------0.04 --0.0089 --------Page 23 of 1 00 MSW 101 mg/L Pho s p h ate ----

! I 1 1 i l l Page 24 of 1 00 Sequoyah Nuclear Plant Biomonitoring May 03-10,2011 AppendixC Chain of Custody Records and Toxicity Test Bench Sheets BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 ofl Client: TVA Environmental Testing Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery 28801 . General Comments: Facility Sampled: Sequoyah NP Ray Duncan: NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell /Fax: 1 Sara Snyder. .let By: __ AL-" 828-3 50-93 68 Metals samples filtered and stored. Samples remained on ice through out sampling and transport to Jab. "'eld Identification I C -/, Container Flow fi\Oj Rt:t '1-ao8 . .. Collection Date/Time ' Sample Description Number& MGD Rain Event? . Volume (Mark as Appropriate) Laboratory Use 'i Collected . - Date Tune Yes If Yes , No Trace IITS Log Arrival By Tune v . Inches Number Temp. 1_ auce , ,;:_ ' ' . * . ..... E-r ,/ -(OC) £1"" : --. SQN-10 1-TOX Comp 5/J /II -S/<J.!,, oUtt'-c7a 2 (2.5gal) / lO!bl.OI o.1.o.8'C .... c;iV ,*:: SQN-INTAKE-Comp s/1111 -s-/OV,! I (2.5 gal) NA .1ro$Dl. oz. 1.rc_ l*"' ";k -, TOX -Sample Custody-Fill In From Top Down _.,. Relinquished By (Signature): Dateffime Received By (Signature): effrme ' -Nf\ 05/0211 l lt>F7 Sonic Delivery .JGI. L 05/02111 10. £\ E\ Sonic /J /( ..£a_ 05/0211 l ETS r'\,.J"-05/02111 l'Sl't.. E:\ l51'2 ..... £'{" , '-./f Instructions

Clients should fiiJ in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time t o initiate testing within that time frame. Samples shipped overnight on Frida y via FedEx or UPS must be marked for Saturday delivery or the y will not arrive until the foUowing Monday. Page 25 of 1 00

... ,. *--..*..* .... _ ... ""'-*-******-* .. .. .......... .. * *.* _ .. ... **** .. ,..,.,,..,., .......... ... --=* .. ...... .*. _ .. ETS

  • Q . llo) J . . . ,i Page_i\ Wbole Emuent Toxicity Sample Receipt Log j Dale Tlrue Received Received Sample Project Sample Sample aame aad desuiptioa State Commeats received n:ceived by from temperature

("C) number aamber 05-m-11 1512 J. Sumner TVA Courier 0.7 0.8 7068 110502.01 TV A Sequoyah Nuclear Plant-101 TN 05-m-ll 1512 J. Sun>ne< TVA Courier 1.2 7068 110502.02 TV A

  • Sequoyah Nuclear Plant -Intake TN Page 26 of 100 BIOMONITORING CHAIN OF CUSTODY RECORD Page 1oft C lien t TVA Environmental Testing Solution , Inc. Delivered By (Circle One): -Project Name: Sequoyah NP Toxicity 351 Depot Street Fed.Ex UPS Bus Client P.O. Number: N/A Asheville , NC Other (specify):

Sonic De l ivery Facility Sampled: Sequoyah NP 28801 General Comments: Ray Duncan: NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell Sara Snyder: Fax: 828-3 5 0-93 68 Metals samples filtered and stored. Samples remairied on a-. /l17L ,. :;::_ --ice through o u t sampling and transport to lab. Fjld Ident i fi cation I tlra&Comp. l../' ?/Collection Dateffime F l ow --., -. Container Sample Description Number& MGD Rain Eve n t? Volume (Mark as App rop riate) 'lDaQ Laboratory Use . . . . Collected . lo; "'Jffii>.il£ .. Date Time " Yes If Yes, No Trace L.EJ"SLog . Arrival By J"ime-.. '} '£r Inches 'Nmnber . Temp. t""r . -&nee *

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Comp 6$/P?.j il- ... 2 (2.5gal) / --o.1 o.'\"{. ]bl-Jtl ,22.

  • j. J-j?,'{ iJ7DP -*" ,-;;

Comp 6S/ CJ>jo'fj If 1 (2.5 gal) NA v -.-#_ *; o.t-L .. "" *::-, *2'L TOX-0':/}0 . -Sample Custody-FiiJ In From Top Down t-.JT"IV..'t. Relinquished By (Signature): Date/Time Received By {Signature): IN "-\L r.Ntll.ftat e/Time d '-* 05/04111 i 'C c ,... Sonic I( .2i_:t... 05/0411 I /().'1'7 s-r Sonic Derfvery 05/04111 ETS / '\""'"/ (Mt * -05/04/1 I t'-13\ E\ 1'l31 Er '-./ t In structions: Clients shou ld fill in all areas except those in the "Laboratory Use"" b l ock. Biomonitoring samples are preserved by storing them at 6"C and shipping them in ice. The hold time for ea.ch sample is 36 hours from the time of collection. Therefore, p l ease collect and ship in such a way that the labora tory will receive the samp l es with ample time to initiate testing within that time ftame. Samp l es sh ipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until t he following Monday. Page 27 of 1 00 .ETS *

  • 0111 ' * *
  • L" v :-.... -*. . ::-lf.f Date received 05-04-05-04-*o5-04-05-04-05-04-05-04-1 05-04-1 05-04-1 05-04-1 05-04-11 05-04-1 05-04-1 05-04-11 05-04-11 05-04-11 05-04-11 05-04-05-04-1 05-04-1: 05-04-05-04-1 r ... e received 0955 09! 0955 0955 0955 0955 0955 0955 0955 0955 0955 0955 0955 0955 1000 1()(}!! 1000 1000 1000 !26 1431 1437 :eenan :eenan :eenan .Keenan .Keenan . Keenan . Keenan .Keenan Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K Keenan K. Keenan K. Keenan K Keenan .Sumner .Sumner Page 28 of 1 00 Received from Fee Fee Fee Fee Feel-Ex Feel-Ex Fed -Ex Feel-Ex Feel-Ex Fed-Ex Fed-Ex Fed-Ex Fed -Ex Fed-Ex UPS UPS UPS UPS IPS Dash Courier TVA Courier TVA Courier [, Whole Effluent Toxicity Sample Receipt Log 1.4 .3 .2 1.2 1.0 25 2.5 1.0 1.0 1.0 25 .4 0.9 0.6 0.5 0.5 05 a: 0.6 05 07/09 08 :::

[SaJDPi2 WIIUe aad description 7076 110504.01 IDuke' creel< 707< 110504.02 7078 110504.03 Proams 7078 110504.04 ProJUeSS 7079 10504.05 *-i>.l iiiSWWTP 7080 Uruted I WWll' 7081 110504.07 United Water-!k:odand Neck WWTP 708: 10504.08 7083 10504.09-7084 110504.10 UnitedWater-7085 110504. 7086 II 0504.12 Pro...-ess Bneri<V Carolinas -Shearon Harris B&B Center 7087 110504. Carolinas -Shearon -Harris Plant 7088 110504.1*f 7089 II 0504.15 Microbac I 1-I , WWTP 7090 110504.16 Microhac I 7091 I 10504 10 Mir=h=. . IP "'p*-* Wm.ram WWTP 709:i 110504.18 Mn* .ead 7093 IIOS04.f9

  • -Enl!eii)ard\1/TP 7094 I 10504 ?0 m* lA . Moonn WWTP 7068 110504 N".
  • Senunvoh Nuolear Phmt. 10 7068 110504 ;v,
  • Seouovah Nuolear Plant* Intake .. I State NC NC NC NC NC NC NC Nt Nt NC IN IN 1...;., 0 1"-...J IL. * "-'"'-* , , --"....:........:...II:...::.JL-L
  • Jt, "-'** _, ** t '""I. ,_) PageJ3 CoiBDlOJlla SOP 04 -Exhibit 04.2, revision 06-29-09 Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number: TN0026450 SQN-INTAKETOX Comp Relinqu i shed By (Signature):

Sonic 05/0611 I 05/06111 '**-.-BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 ofl Environmental Testing Solution, Inc. 351 Depot Street. Asheville, NC 28801 Phone: 828-350-9364 Fax: 828-350-9368 Delivered By (Circle One 4 FedEx UPS Bus Client Other (specify): General Comments: Ray Duncan: ___________ _ Ben Mitchell ' Metals samp l es filtered and stored. Samples remained on ice through out sampling and transport to lab. Container Number& Flow MGD R ai n Event? (Mark as Appropriate) Laboratory Use I f Yes. Inches Sonic Delivery .,1 ETS No Trace ETS_Log Number 05/06/11 05/06111 _J:*j_r-ET IS Instructions: Clients should fill in all areas except those in the '"'Laboratory block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in i ce. The hold time for each sample is 36 hours from the time of collection. Therefore , please collect and ship in such a way that the laboratory will receive the samples with amp l e time to initiate testing with i n that time frame. Samples shipped overnight on Friday via Fed Ex or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. P age 29 of 1 00 ..... L. ..... **-*****----- .. ---***-... _ ....... i* : t *. ,., L -J Page_.J!l Whole Emuent Toxicity Sample Receipt Log Dale Time Received Received Sample Project Sample Sample Dllllle aad dacriplioa State CoBUBeJlts received I"Keived by from lempe<alure ("C) aumber number OS-06-11 0946 K.Keenan UPS 3.3 7089 110506.01 Microbac Environmental -Roc:kingham WWTP NC OS-06-11 0946 K. Keenan UPS 3.3 7090 110506.02 Microbac Environmental -Roseboro WWTP NC OS-06-11 0946 K.Keenan UPS 3.3 7091 110506.03 Microbac.Environmental-Westooint-w ......... WWTP NC OS-06-11 1000 K.Keenan Fed-Ex 1.9 7076 110506.04 Duke Energy Carolinas -Belews Creek Sleam Station NC OS-06-11 1000 K.Keenan Fed-Ex 2.3 7077 110506.05 Bladenboro WWTP NC OS-06-11 1000 K.Keenan Fed-Ex 2.S 7078 110506.06 Carolinas-Cane Fear S.E. NC 05-06-11 1000 K.Keenan Fed-Ex 2.S 7078 110506.07 Prowess Enenw Carolinas -Cepe Fear S.E. -Upstream/lntake NC OS-06-11 1000 K.Keenan Fed-Ex 2.7 7079 110506.08 Citv of Gastonia -Dallas WWTP NC 05-06-ll 1000 K. Keenan Fed-Ex 2.0 7080 110506.09 United Wa!er-Enfield WWTP NC 05-06-11 1000 K. Keenan Fed-Ex 2.0 7081 110506.10 United Water-Scotland Neclc WWTP NC OS-06-11 1000 K. Keenan Fed-Ex 0.8 7082 110506.11 WilaonWWTP NC OS-06-11 !000 K.Keenan Fed-Ex 1.8 7083 110506.12 Elementis NC OS-06-l! 1000 K.Keenan Fed-Ex 0.9 7084 110506.13 United Water-Farmville WWTP NC 05-06-11 1232 K. Keenan Dash Courier 1.3 7094 110506.14 OW ASA-Mason Farms WWTP NC 05-06-11 ISIS J. Sumner TVA Courier 0.9,0.9, I.S 7068 110S06.1S TVA-se<luo'WilNuclearPiant-101 1N OS-06-11 ISIS J. Sumner TVA Courier 0.9 7068 IIOS06.16 TV A -Sequovah Nuclear Plant-Inlllke 1N Page 30 of 100 SOP 04 -Exhibit 04.2, revision 06-29-09 I I I I I I I I I I I I I I I ' *eTS * :t ' ., ) -:**.:,1 ,......__..,. ),' .) Environmental Tutlng Solutions, Page I of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Nuclear Plant NPDES #: Project#: _......:,"""0'""\o:,_,&,.___ ___ _ Dilution preparation information: Dilution prep (%) 10.8 21.6 432 Effiuent volume (mL) 270 540 1080 Diluent volume (mL) 2230 1960 1420 Total volume (mL) 2500 2500 2500 Test organism information: ' 86.4 2160 340 2500 Organism age: '1."2..'2..S -. 71 tlo.Jrt.":, _0\.b Date and times organisms os*<n.*t\ \\..00 were born between: 100 2500 0 2500 Organism source: A TO-t. l> f

  • en-* \ Transfer bowl information:

pH= , .\o 8' s.u. Temperature = 1."l."'L oc Average transfer volwne: o. Daily feeding and renewal information: County: t-\f\to\\\.."ttN Outfall: 101 Comments: Test information: Randomizing template: '-1-l-LLOw Incubator number and ..3(. shelf location: Artemia CHM number: Drying information for weight determination: Date I Time in oven: lo_'ft.IOo\ \ 1'-liO Initial oven temperature: \oO *( Date I Time out of oven: Final oven tell'l2_erature: Total drying time: 1."'*1411JI2S Day Date Morning feeding Afternoon feedin g Test initiation, renewal, or Sample number s used MHSW batch 0 2 3 4 5 6 7 Page 31 of 100 SOP AT20-Exhibit AT2.0.3, revision 04-0l-09 I I I I -I I I -I I I I I I I I I I -I I aETS . . 9. ; . ' . ) ',. ... , r---'. Page 2 of6 .) Env!ranmantal 'll!sllng Salutionr,lnc. Species: Pimeohales promelas Client: TVA I Sequoyah Nuclear Plant, OutfalllOl, Non-treated Date: \ s .....

  • and Growth Data Day t:UNTKUL l0.8% _21.6%_ A B c D E F G H I 'J 0 10 /0 10 ID to -10 /0 IO /Q /0 l I() 10 to If:) /0 tO /0 IC 10 /0 2 /0 ft> 10 tO ({) /0 IC ro f() 10 3 10 I() /C) ro IO ID IO /Q 10 4 f() '0 IQ It> l() 10 If) to IO 10 5 lo /0 10 IO IC 16 lD 10 If:) 10 6 10 /Q "'"' /D 10 10 /Q 10 10 10 7 IC> ID ro {CJ IC /(J q /Q A -Pan weight (mg) Tray color code::

Swt Analyst: 11.S7 14.1>'-14-.'!4 ,,,1'\ t-?,.6'! 14.01 \t,.&? Date: Q:Z *Qtl:*l \ B = Pan + Larvae weight (mg) Analyst: mlt£ It,\.

  • --_,

12-t..'tS u..l4't lt.&.&o Date: u.ic u.;, C = Larvae weight (mg) = B-A Hand calculated

  • .>J 1!\C\ &.IC\ ,.'{'\

1&.4\ j1.Co2 (,.SC\ c.tb Analyst: --Weight per initial number of larvae (mg) = C /Initial number of larvae Hand calculated

  • .A/

\'bfl\ ,....o.o . "' Q; \: Analyst: r-o* o* o* o* t>' ()' o* ()* C)' -Average weight per Percent reduction initial number of from control(%) o.'11b o.ss \ -l{).\.1. larvae (mg) Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: _!{ L 10 tO ID ./Q /0 10 IC 10 to to IO 10 10 10 l(J t!J."lt l4-M ["\. ,,., ()* -g,'-{7. Page 32 of 1 00 SOP A T20 -Exhibit A T20.3, revision 04-01-09 I I I I -I I I -I I I I -I I I I I I -I I .ETS * . ' ' '. *._ ' )**?-_,.-' .-- Page 3 of6 ) Envl1011mtntaiTesdng Solutions. Inc. Species: Pimephales promelas Client: TV A I Seauoyah Nuclear Plant, Outfall lOt, Non-treated Date: os. at.* t \ Day 0 1 2 3 4 5 6 7 A= Pan weight (mg) Tray color code:: Analyst: Date: __ __,o...,;,._

  • ..,'>>r.:..*.u.\l

__ _ B =Pan+ Larvae weight (mg) Analyst: M ltf Date: 05 * *f*ll C =Larvae weight (mg) = B-A Hand calculated()} Analyst: ..--Weight per Initial number oflarvae (mg) = C /Initial number of larvae Hand calculated. / Analyst: c)1 v Average weight per Percent reduction initial number of from control (%) larvae(me:) 43.2% M N 0 fD IC ID 10 IO 10 /0 /{) /fj /Q /Q 10 IO IO 10 10 10 10 10 to I() IO lC o.t1s Survival and Growth Data 86.4% 100% P Q R S T u v w X 10 IO *IC 10 l'() IO IO IO 10 10 ID tO ID to ro to 10 .10 I 0 /0 I 0 I 0 (0 10 10 I'{) 10 I() IQ fO IO IC ID IO IQ IO IC I() IC IC ID IO 10 lO to /0 fD t() t() ID 10 tO 10 t() 10 10 10 10 LO I() ID fO tO 10 10 fQ (Q tO ft:J IQ 10 IO 0.'"1 '\ \ o.1'lo Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: Page 33 of 100 SOP A T20-Exhibit AT20.3, revision 04-01-09 I -I I I I I I I I I I -I I* *eys * , . : \) . .. . ... ,.__) ... ) --** ' .. ) E nviron menti iT esting Solutions, Inc. Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant, OutfalllOl, Non-treated Date: OS*U2. \\ Day 0 1 2 3 4 5 6 7 A= Pan weight (mg) Tray color code:: Analyst: ___ ..1!1\'+A\ltu...P ___ _ Date: 1 .... 1 ___ _ 8 =Pan + Larvae weight (mg) Analyst: M. tte Date: pc;. 14:*\1 C = Larvae weight (mg) = 8-A Hand calculated. l Analyst: d-..__ Weight per initial number of larvae (mg) = C I lnitial number of larvae Hand calculated. J Analyst: cJ _

  • Average weight per Percent reduction initial number of from co n trol(%) larvae lb 10 ID to 10 ID ro /D. /0 to /D /0 10 /0 It) 0 .'1 iD I D. tO /0 lO lD ro I\ ()' /0 Comment codes: c -= clear, d -= dead, fg = fungus , k = killed, m =missing, sk =sick, sm = unusually small, lg = unusually large , d&r = decanted and returned, w = wounded. Comments:

Page 4 of6 Page 34 of 1 00 SOP AT20-Exhibit AT20.3, revision 04-01-09

  • * *
  • Environmental Testing Solutions, Inc. C....'"""" l%1 .........

C....lnld ltl.S% 21.6% 43.2% 86.4% 100% 110'% l obike Oulf.di!OI

Dcuoadt"s MSD YAhoo
PlllSD: .... a..: o..,a dt's i\lSD value; PMSD: A B c D E F G H I J K L lll N 0 p R s T u v w X y 7. AA BB w.w..-or ..... 10 10 1 0 1 0 10 10 1 0 1 0 10 10 1 0 1 0 10 10 1 0 10 10 10 10 10 10 10 10 10 10 10 10 10 0.11 96 15..5 0.1052 IJ.7 Pag e 35 of 1 00 n..aJ .-bt:t flf &an-.1 1 0 1 0 10 10 10 10 10 10 9 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 7 A*'--fPI(")

14.&5 1 4.o6 1434 13.1 9 13.36 13.89 13.81 12.55 1 4.01 12.85 11.91 14.4 9 1}.54 13.92 1}.23 II 74 1}_8) 1}.79 15.00 14.76 B.63 13.44 13.4 9 12..97 1 4.J5 ll.JI 1 4.4 5 12.24 MSO* PMSD= TV A I Sequoyah Nuclear Outfall lOt Non-treated May 03-10,2011 Pimephales prom das C h ro nk Wbole Em uen t Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Cont r ol Ve rifieat io n of Data E ntry, Calculatio n s, and Statistical Analyses Not foe Compliance 1Dt.emal Labonllaoy QC B*Pu+t...n. Lan-.....tpt(...:l _,....., Codlldmtot..- W<IPJI._,_ ..... (..., *A** ...a.a-ce.t.n. ,.., S ........ auallu fli ............. ., ...... ("&) luY*(") 21.89 7.04 0.704 0704 21.62 7.s6 0.756 0.770 6.6 0756 22.3) 7.99 0.799 0.199 21.)8 8.19 0.819 0819 21.85 8.49 0.84 9 0849 22.78 8.89 0.859 0.851 70 0.889 2279 8.98 0.898 0898 20.23 7.68 0.768 0768 20.60 6.59 0.7)2 0.659 2171 886 0.886 0..853 9.6 0.886 2l.04 9.1) 0.913 0.913 23.28 87 9 0.819 0.819 21.15 761 0.761 0.761 22.36 8.44 0.844 OJI2S S.2 0.844 21.64 841 0.841 0 841 20.27 I.Sl 0.8 53 0.&53 22.21 845 0.84S 0.84S 21.84 0.791 6.S 22.16 7.16 0.71 6 0.71 6 22.7) 7'17 0.7'17 0.7'17 21.64 8 01 0.801 0.80 1 20.40 6.96 0696 0790 88 0696 22.12 863 0.863 0863 2097 8.00 0.800 0800 23.28 8.93 0.8 93 0.893 21.32 7.94 0.794 0..&5 1 9 4 0.794 22.1 9 7.7 4 0..774 0774 18.84 660 0943 0.660 Mintmum SigrufiC8r11 Doff.....,.., Pm:eot Miaimum Sig<Jiiicanl Oiffc:m><e ... __ "'--"" __ ___ Project awaber. 7061 lluiltlll"'i'f-al .......... ,._, c-. or PucuJ rwt.diM ,,.. (%) __ .,...._ y aftdioe "*-...... malnll (%' (-c) ................ ..,.,. f%) IOCI.O 0.770 6.6 Nor appljcahle IOCI.O lUlSI 7.0 -111.6 !n.S o..sJ.4 14.1 -&.4 100.0 O..S25 S.l -7.2 100.0 0.791 6..S -U 100.0 0.798 8.1 -2..7 n..5 0.7110 12.2 -1.4 PMSO IS

  • meosureofle:sl pRCUIOIL 1be PMSD is lheminimum pcn:at1 differatce -lhecontrcl and tr_,_ char C3l be dedsal stab51icallysipif-.atn a wbol eeffiuati iOJOCIIy leSt. U...-u P MSO bound clelammed by USEP A (I Olb -ule)
  • 12%. l'-PMS.O bowxl US.EPA (901h pm:cntile)
  • 30%. Low..-and upper PM S O boundswcrcdcu::nuincd liom lbe IOlb pacentile, of PMSD d.Ufrom EPA's WET 200 1 a; USEP A. 2 00lb). Ftle: ...,101_050311dala.ldsx Enlerecl by: J.

Reviewed by: --o::- .ETS * *

  • u ,,_,. .....

.. ... _: .:..t._-;r:::.-*

.) Environmental Testing Solutions.

Inc. Start Date: 5/3/2011 End Date: 5110/2011 Sample Date: Comments: Cone-% 2 0-Control 0.7040 0.7560 10.8 0.8490 0.8890 21.6 0.6590 0.8860 43.2 0.7610 0.8440 86.4 0.8450 0.8050 100 0.8010 0.6960 Intake 0.8930 0.7940 Cone-% Mean N-Mean 0-Control 0.7695 LOOOO 10.8 0.8510 Ll059 21.6 0.8343 1.0841 43.2 0.8248 1.0718 86.4 0.7908 1.0276 100 0.7900 1.0266 Intake 0.7803 1.0140 Auxili!!!I Tests J * " ** J. '-"' TV A I Sequoyah Nuclear Plant, Outfall101 Non-treated May 03-10, 2011 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth TestiD: PpFRCR SampleiD: TV A I Sequoyah Nuclear Plant, Outfall 101 LabiD: ETS-Envir. Testing SoL Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas 3 4 0.7990 0.8190 0.8980 0.7680 0.9130 0.8790 0.8410 0.8530 0.7160 0.7970 0.8630 0.8000 0.7740 0.6600 Transform: Untransformed 1-Tailed Isotonic Mean Min Max CV% N t-Stat Critical MSD Mean 0.7695 0.7040 0.8190 6.623 4 0.8199 0.8510 0.7680 0.8980 6.967 4 -1.643 2.410 0.1196 0.8199 0.8343 0.6590 0.9130 14.114 4 -1.305 2.410 0.1196 . 0.8199 0.8248 0.7610 0.8530 5.190 4 -1.114 2.410 0.1196 0.8199 0.7908 0.7160 0.8450 6.839 4 -0.428 2.410 0.1196 0.7908 0.7900 0.6960 0.8630 8.766 4 -0.413 2.410 0.1196 0.7900 0.7803 0.6600 0.8930 12.249 4 Statistic Critical Skew N-Mean LOOOO LOOOO LOOOO LOOOO 0.9645 0.9636 Kurt Shapiro-Wilk's Test indicates normal distribution (p >0.01) 0.88409168 0.884 -1.217221232 1.2704004 Bartlett's Test indicates variances (!;! = 0.56) Hypothesis Test (1-tail, 0.05) Dunnett's Test Treatments vs 0-Control Point IC05 ICIO ICI5 IC20 IC40 IC50 % >100 >100 >100 >100 >tool >100 >100 Page 36 of 1 00 NOEC LOEC 100 >100 so 95%CL{Exp) 3.9158771 ChV TU MSDu 1 0.11955186 Linear Interpolation {200 Resamples) Skew MSQ2 0.15536303 15.0863171 MSB MSE 0.00391794 0.00492163 F-Prob df 0.566574454 5, 18 File: sqnl01_0503lldata.xlsx Entered by: J. Sumner Reviewed by:---+-- . .. .. .. .. I TVA I Sequoyah Nuclear Plant, O utfalllOl-Intake N o n-treated May 03-10,2011 .ETS * * . . . J ;: -. . --. *. Statistical Analyses .,) Environmental Testing Sol u tions, Inc. Larval Fish Growth and Survival Test-7 Day Growth Start Date: 513120 I I End Dat e: 5/1012011 Sample Date: Comments: Non-treated Cone-% I D-Control 0.7040 10.8 0.8490 21.6 43.2 86.4 100 Intake Cone-% D-Control 10 .. 8 21.6 43.2 86.4 100 Lntake Auxiliary Tests 0.6590 0.7610 0.8450 0.8010 0.8930 Mean 0.7695 0.8510 0.8343 0.8248 0.7908 0.7900 0.7803 2 0.7560 0.8890 0.8860 0.8440 0.8050 0.6960 0.7940 N-Mean 1.0000 1.1059 1.0841 1.0718 1.0276 1.0266 1.0140 TestiD: Lab ID: Prot ocol: 3 0.7990 0.8980 0.9130 0.8410 0.7160 0.8630 0.7740 Mean 0.7695 0.8510 0.8343 0.8248 0.7908 0.7900 0.7803 PpFRCR Sample ID: ETS-Envir. Testing Sol. Sample Type: FWCHR-EPA-821-R-02-013 Test Species: 4 0.8190 0.7680 0.8790 0.8530 0.7970 0.8000 0.6600 Transform: Uotransfonned Min Max CV% 0.7040 0.8190 6.623 0.7680 0.8980 6.967 0.6590 0.9130 14.114 0.7610 0.8530 5.190 0.7160 0.8450 6.839 0.6960 0.8630 8.766 0.6600 0.8930 12.249 N 4 4 4 4 4 4 4 Statistic Shapiro-Wilk's Test indicates normal distribution (p > 0.0 I) 0.98316872 F-Test indicates equal variances (p = 0.33) 3.51607203 Hypothesis Test (1-tail, 0.05) MSDu Homoscedastic t Test indicates oo significant differences 0.10523383 T r eatments vs D-Co o trol Page 37 of 100 t-S tat -0.199 MSDp 0.13675612 TV A I Sequoyah Nuclear Plant, Outfall I 0 I -intake DMR-Discharge Monitoring Report PP-Pimepbales promelas !-Tailed Critical 1.943 Critical 0.749 47.4683456 MSB 0.00023113 MSD 0.1052 Skew Kurt -0.2349007 0.4704176 MSE F-Prob df 0.00586562 0.84920698 1, 6 File: sqn 1 0 1_ 050311 data.x.Jsx Entered by: J. Sumner Rev iewed by: Co otrol 10.8% 43.2% 86.4% 100% lot a kr Page 38 of 1 00 TVA I Sequoyah Nuclear Plant, Outfall 101-Non-treated May 03-10, 2011 Pimephales promelas Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses F il e: sqn10 1_050311 che m.xls Ent er ed by: S.drn s R eviewed by: I l I i I i j I J ' : I I I I ' I I ....: I I I I I I I I I I -I I ) ill' ' ) I Page 5 of6 ) EnvfronmentaiT.,l l ng Sofudonc. Inc. Specie s: Pimephales promelas Date: OS* CS!>* \\ C lien t: TV A I Seguoyah N uclear Plant, Outfall lOt , No n-treated CONTROL Non-treated 10.8% 21.6% 43.2% 86.4% 1 00% 100% Intake Page 39 of 1 00 SOP AT20-Exhibit A T20.3, revision 04-0 1-09 ol'\ tteAt-lA\.'Iteb r ()/-- r I Page 6 of6 ' I ) . L_.J ' * ) Environmental Testing Solutions, Inc. I ], . ! I ! l l 1 a I I r Conceo-l I tration I CONTROL Non-treated I I 10.8% I 21.6% I I 43.2% I 86.4% I I 100% I I ' 100% Intake Page 40 of 1 00 SOP A T20-Exhibit A T20.3 , revision 04-01-09 I I I I I I I I I I I --I I Page 1 of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: Tennessee Valley Authority Facility: Seguoyah Nuclear Plant NPDES #: TN0026450 Project#: __ .... l ____ _ Dilution preparation information: Dilution prep (%) 10.8 21.6 Effluent volume (mL) 270 540 Diluent volume (mL) 2230 1960 43.2 1080 1420 Total volume (mL) 2500 2500 2500 Test organism source information: ' 86.4 2160 340 2500 Organism age: < 24-hours old Date and times organisms were born between: 05-&l\ 6,\<<a TO Culture board: 100 2500 0 2500 \'1.()1:) Replicate number: 1 1 2 1 3 4jSj6j7jBj9 10 Culture board cup number: q I, 1\ I \?;.I tl.\ I 'OS II& I'll Transfer vessel information: pH-I* 00 S.U. Averaae transfer volume (mL): I Daily renewal information: Day Date Test initiation and feeding, renewal and feeding, or termination time 0 2 3 4 5 6 7 Temperature-"J..t.l.411 MHSW batch used lS oc County: Hamilton Outfall: 101 Comments: Test information: Randomizing template color: Incubator number and shelf location: YWTbatch: Selenastrum batch: 6-ftLb O'l*1.\*l\ o't*t\-1\ Analyst Control information: Summary of test endpoints: Control-I Control-2 Acceptance criteria % of Male Adults: QT. ()1. S:20% 7-day LC 50 '71001. %Adults having 3ra Broods: 1001 IDol. :<:80% NOEC tcc7. % Mortality:

07. 01 .. S:20% LOEC ') tOC1 .. Mean Offspring/Female:

-,q,\ '2.'\.1 :<: I 5. 0 offspring/female ChV '>I l'\l\1_ %CV: .... 1.1 .. ,.n. <40.0% ICzs "1I007o Page 41 of 100 SOP A Tll -Exhibit AT 11.2, revision 04-01-09 i ,. il t il l I I .. I 1 I I -I I I I I I I I I I -I I Page2of7 ) lirwlroameDUfftsdngSolWons.lnt. Species: Ceriodaohnia dubia Client: TV A I Seguovab Nuclear Plant. Outfall lOt Date: \....__ __ _ CONTROL-I Survival and Reproduction Data Replicate number Day 1 z 3 4 5 6 7 8 9 10 l Young produced a a l} ('\ a a 0 0 0 0 Adult mortality L-L \...... L L \...... L... L \...... '-2 Young produced (')* 0. 0 D 0 0 D 0 0 0 Adult mortality \.... L-'-\.... \.._ L. '--\.... '-3 Young produced c c c D c 0 c c 0 0 Adult mortality L... '--L-L-'--'-'-4 Young produced '\ .;, c.( s '-\ '-t \{ 't '-l Adult mortality '--'--'-'-.._ '--\..._ L. '--L.. s Young produced \0 \\ ,, \1.. \\ l'l-l1.. lD l"L 11... Adult mortality L. L '-\_ '--L '--L L. '--6 Young produced () 0 c D 0 c D Cl C\ 0 Adult mortality L \...._ \._ L-\.... \._. L L L '-7 Young produced \'-l 1'-4 t\., \'S ,, \'\ \S Total young produced '2.8. 3\ 3\ '1.'\ 'ti Final Adult Mortality \. \. '-'--'-\ \..... '--'--'-X for 3"' Broods )(_ _)(. }(.. "').(_ >5-X ><. il<-. ")<:. Note. Adult mot1B11ty (L = hve, D =dead), SB =split brood (Single brood spht between two days), CO a cany over (of!Spnng earned over wtth edult dunng tr&IISfer). Concentration: % Mortality: I 07. Mean Offspring/Female: I '2.'\.1 CONC* 10.8% Survival and Reproduction Data Replicate number Day 1 2 3 4 s 6 7' 8 9 10 1 Young produced () () 0* 0 0 0 0 0 0 () Adult mortality L L.. \..... \._ '-\.._ \.._ L '-\..-2 Young produced 0 0 c 0 0 0 Q 0 0 a Adult mortality L '--'--'-'-L L. '--\..... '--3 Young produced D (:) 0 t) _0 c 0 0 0 D Adult mortality L \... L. '-L \.... L \,_ \...... L. 4 Young produced '5 "5 "\ s '1 '-\ '1 t..'* Adult mortality \.._ '-L L.. '-L L '-L.. '--s Young produced ro l"l. 10 1"2.. ll. tO ll 1\ Adult mortality \..... '-\.... L '-L L \..._ \... L 6 Young produced 0 n 0 0 (\ 6 () (') 0 0 Adult mortality L. L L-L L... L L.. L L L 7 Young produced n \S \\c, \\o 'o \\o '""" l'-\ l5 \S Total young produced 1.1 3() "Z.i 3() Final Adult Mortality \ L '-L L L L L.. '--"-Note: Adult mortality (L D m deed), SB -split brood (single brood split between rwo days), CO= can over (of!Spring carried over with adult during transfer . Concentration: % Mortality:

07. Mean Offspring/Female:

3o.'l %Reduction from Control-!:

  • C.(,C7o Page 42 of 1 00 SOP A Tll -Exhibit A Tll.2, revision 04-01-09 I -i I i I I I I I I I I I --I I

)!; j d .) &nvlnm:ntenUJTutlngSoM!ons.II'IC. Species: Ceriodaohnia dubia Page 3 of7 Client: TVA I Sequoyah Nuclear Plant. Outfall lOt Date: ___,Oi...,* ___ _ coNe* 21.6% Survival and Reproduction Data . Replicate number Day I :z 3 4 s 6 7 8 ' 10 1 Young produced (\ 0 C'J 0 0 _0_ 0 0 0 a. Adult mortality L L-'-L.. L L. L L... L L... :z Young produced D 0 D c (") D 0 0 0 () Adult mortality L-\. L. L L. '--'--L. '--L... 3 Young produced D 0 D c b .() 0 c 0 0 Adult mortality '-L. \.... '-L-L '--'-'-'--4 Young produced "-\ '-\ . '-\ 0 '-\. '-( '-\. '4 "( Adult mortality L. '-'--'-'--'-'-\..:... \.._.. '--s Young produced \"t \0 \"L ,:, l1... l'l.. \'1 l"L I\ 1\ Adult mortality L L L.. L \..__ L '-L '--'--6 Young produced 0 0 c D 0 0 D 0 0 0 Adult mortality \...... \_. '-L-L L. L '-L \.._ 7 Young produced ,, ,., '" tS -Total young produced ,!, '1. 3\ '2.."\ 3! :!.\ 3\ 3\ Final Adult Mortality '-'--'--'--' '--L.... '-'-\.,..._ Note: Adult mortality (L = rwe. D =dead), SB =split brood (single brood spOt between two days), CO

  • can over offSpring carried over witb aduh during transfer . Concentration:

% Mortality:

07. Mean Offspring/Female:

% Reduction from Control-1: -,,**\"1. CONC: 43.2% Survival and Reproduction Data Replicate number Day 1 :z 3 4 5 6 7 8 ' 10 1 Young produced 0 0 0 0 () 0 0 0 0 0 Adult mortality L-L. L L L L L L L L. :z Young produced 0 C) c D 0 0 D a 0 0 Adult mortality '--'--\._ \........ L L L. "-'--'--3 Young produced c 0 a 0 0 0 c 0 0 0 Adult mortality L. L-L.. \...... L-'--L L. '-L. 4 Young produced '-\ s "' '-1 '-\ '"l "t Adult mortality \........ \... L. \...... L. L L L-L '-s Young produced n \'l,. '"' t\ 11. '1 .. *3 l"L Adult mortality L L '-L L L L. \.... L_ 6 Young produced 0 c C'1 0 0 0 0 0 0 0 Adult mortality L \._ L L \._ L L L L l. 7 Young produced &(,. l4\ "Z.O \"'\ \S \Q Total young produced 3"1 al. Final Adult Mortality L. L. '-L.... L-\....... '-L \....... \........ Note: Adult mortality (L e live, D a dead), SB B split brood (single brood split between two days), CO e can over offSpring carried over adult during transfer , Concentration: % Mortality:

07. Mean Offspring/Female:

3'?l.l %Reduction from Control-I: -r!..t1 .. Page 43 of 1 00 SOP A Til -Exhibit A Tll.2, revision 04-01-09 ..... "'! I I ' I I l I I I l I I II j J I I I I I I I I I I --I I Page4 of7 Species: Cerlodaphnia dub/a Client: TVA I Seguoyah Nuclear Plant. OutfalllOl Date: coNe* 86.4% Survival and Reproduction Data . Reolicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 () 0 0 c 0 Q Adult mortality '--'-\..... L L.. L L L '-L 2 Young produced 0 0 0 0 0 0 0 0 c 0 Adult mortality \..... . '-'--L. '---'-\...... \..... L. '-3 Young produced c D D () 0 0 _C) 0 c a Adult mortality L L-'-* '-'-*I..... \.._ '--L '--4 Young produced s s s '-\ "---"i \o s Adult mortality L-'-\.... '--'--'--L. L.... L L. 5 Young produced \"l \1.. \4 \l. \\ \\ \L. ,,_ Adult mortality L. L.. L \.... L L. \._. \_ \..... L 6 Young produced r'l D () 0 c () D b 0 D Adult mortality -L L.. '-'-'--L L '--L-L 7 Young produced \lo \\D -'"Z.O '1."1.. \'\ \C\ \S \i Total young produced 31 35 35 Final Adult Mortality L. \._ \........ \...... \...... L.. '--'--'--L.. Note: Adult mortality (L = live, D D dead), SB -split brood (single brood split between two clays), CO = car over offSpring earned over with eduh duriog translet' . Concentration: % Mortality:

07. Mean Offspring/Female:

3'-1.'\ % Reduction from Control-I: -l1.'S7. CONC* 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 a 0 0 0 0 c Adult mortality L L L L l J. L L L L 2 Young produced 0 0 0 a 0 0 0 0 _0. D Adult mortality L L L. L L... L L.. '--L L 3 Young produced ('"') 0 0 D (} c (j 0 Adult mortality "'--\_ L. L L '-L. L. '-4 Young produced s 5 la s s '-\ (. 4 s Adult mortality '---\.... '--L '-\.... L L \...... L. 5 Young produced l"' l1.. 11. -' n .. l"t rt.. I'Z... Adult mortality L. L L \._ L L. L L L L 6 Young produced () D (\ 0 () 0 0 c 6 0 Adult mortality L L L L L L L L 1 l 7 Young produced \\ \& La \i ,,, "1..0 l(\ Total young produced 3k 3<o a\ .. 3'-Final Adult Mortality L. L.. L L L L. L L.. L. '---Note: Adult mortality (L live, D dead), SB D split brood (single brood split between two clays), CO -ca over carried over with adult durin_A transfetl, Concentration: %Mortality:

07. Mean Offspring/Female:

.';!.., 1.[ % Reduction from Control-I: Page 44 of 1 00 SOP AT l I -Exhibit AT I 1.2, revision 04-0 I -09 ! :;;;:;qz.;.F"'" l .. j I II I J I I I I I -I I I I I I I I -I I Page 5 of7 Species: Ceriodaohnia dubia Client: TV A I Seguoyah Nuclear Plant, OutfalllOl Date: OS-0* \\ CONTROL-2 Survival and Reproduction Data Replicate number Day l 2 3 4 5 6 7 8 9 10 l Young produced a c a 0 a c c 0 c Q Adult mortality "-'-'--'-\.._... L '-'-\...... \.._ 2 Young produced () i> 0 D a 0 0 0 0 0 mortality \..... L-\..... L-L.... L-L.... \...... \,_ 3 Young produced 0 0 D D t:> 0 () c 0 Adult mortality L.. L. \.....:. '-'-L '-L \..... L... 4 Young produced '-\ '-\ "\ ""' Adult mortality L... '-\..... L '--'-\...... '-'-'-s Young produced \"'l.. tO \"1. \ \ \\ \\ l\ \1. \'L 10 Adult mortality L. \..... L L \.,_ '-'--L L L. 6 Young produced f) 0 c 0 D (J a 0 0 0 Adult mortality L. \...... \...... '--L. L.-'-'--\.._ L 7 Young produced \\ l\o \S \$ \, \S \S Total young produced '2.'\ 'Z.t:. :=o "2.'\ 1.1 3'2. 3\ Final Adult Mortality L-'--\._. \._.. '--L L-\..._ '-X for 3ra Broods X )( )(.. '>L X.. 'X-X: Note. Adult mortality (La live. D =dead), SB spht brood (single brood spilt between two days), CO= carry over (ofl'spnng earned over With adult dunng tnlnslior). Concentration: % Mortality: I 07. Mean Offspring/Female: I CONC: 100% Intake Survival and Reproduction Data Replicate number Day 1 2 3 4 s 6 7 8 9 10 1 Young produced 0 0 0 C\ a () a 0 Q () Adult mortality L l L L L L L L \. '---2 Young produced 0 0 0 0 D {} 0 0 c _Q Adult mortality \..... \..... L L L L. L L '-L. 3 Young produced a () c a Q c 0 0 0 0 Adult mortality l L L.. L.. L. L L. \..... \..... L. 4 Young proauced \.o '"" s "'\ 5 4 \.( lf Adult mortality '--'--L. \...... '--'--'-'--'-'--s Young produced n. " n. ll.. ID II Adult mortality L \--L L \.... L L L L l 6 Young produced 0 0 0 0 () 0 () (\ 0 0 Adult mortality L L '-L L L L L L L 7 Young produced 1.\ l\o (0 "l.D t& \& \'\ "2.\ 1.\ Total young produced 31 3'S 31.. 3\o Final Adult Mortality \._ L-\.. L L L. L. \.,_ L L.. Note: Adult mortality (L-live. D a dead), SB -split brood (single brood split between two days), COs can over ofi'SPrinq carried over with adult durinJI u6nsferl. Concentration: I % Mortality:

07. Mean Offspring/Female:

3S.! % Reduction from Control-2: -"Zo.s7. Page 45 of 1 00 SOP A Til -Exhibit A Tll.2, revision 04-01-09 .ETS * *

  • t..:J . ***ii'i?:c;:
,. ..... ,: .... .) Envii'Dilft>GfltaiTestlng5olullans,lnc.

Control-I Day 1 l 3 1 0 0 0 2 0 0 0 3 0 0 0 4 4 3 4 s 10 11 11 6 0 0 0 7 14 14 16 Total 28 28 31 10.8% ---Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 3 5 5 s 13 10 13 6 0 0 0 7 17 15 16 Total 33 30 34 21.6% ---Day I 2 3 I 0 0 0 2 0 0 0 3 0 0 0 4 4 s 4 s 12 10 12 6 0 0 0 7 17 17 IS Total 33 32 31 43.2% --. -Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 6 4 5 s II 12 14 6 0 0 0 7 19 16 15 Total 36 32 34 Page 46 of 1 00 TV A I Sequoyah Nuclear Plant, OutfalllOl -Non-treated May 03-10,2011 Verification of Ceriodllphnill Reproduction Totals 86.4% Re licate number Total 4 5 6 7 8 9 10 Day Renllcate number I l 3 4 5 6 7 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 5 4 4 4 4 4 40 4 5 4 s 5 4 4 6 12 11 12 12 10 12 12 113 5 13 12 12 14 12 II II 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 15 15 15 14 15 13 13 144 7 16 19 16 16 20 22 19 31 31 31 30 29 29 29 :m_ Total __ 34 35 35 36 37 36 100% Replicate number Total 4 s 6 7 8 9 10 Day R plicate number 1 2 3 4 5 6 7 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 4 5 4 4 4 6 44 4 5 5 6 5 s 4 6 12 10 12 12 10 II II 114 s 14 12 13 13 12 14 12 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 16 13 16 14 14 15 15 lSI 7 19 19 17 19 18 20 18 32 27 33 30 28 30 32 309 Total 38 36 36 37 35 38 36 Control-2 Reolicate number Total 4 5 6 7 8 9 10 Day ReDileate number 1 2 3 4 5 6 7 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 3 4 4 4 4 4 40 4 4 3 5 3 3 3 4 13 12 12 12 12 II II 117 5 12 10 12 II 11 II II 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 19 14 16 IS 15 16 18 162 7 13 13 17 16 IS 13 15 36 29 32 31 31 31 33 319 Total 29 26 34 30 29 27 30 -tOO% Intake Re licate number Total 4 5 6 7 8 9 10 Day Re licate number I 2 3 4 5 6 7 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 4 4 3 4 4 4 42 4 6 4 5 4 5 5 4 II 12 12 13 13 13 12 123 I s 13 12 14 11 12 12 10 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 15 19 20 19 17 IS 18 173 7 18 21 16 20 20 18 18 30 35 36 35 34 32 34 338 I Total 37 37 35 37 35 31 .. I 8 9 0 0 0 0 0 0 4 6 12 12 0 0 19 15 35 33 8 9 0 0 0 0 0 0 6 4 12 12 0 0 18 20 36 36 8 9 0 0 0 0 0 0 3 4 12 12 0 0 17 15 32 31 8 9 0 0 0 0 0 0 4 4 13 13 0 0 19 21 36 38 10 0 0 0 5 12 0 18 35 10 0 0 0 5 12 0 19 36 10 0 0 0 4 10 0 15 29 10 0 0 0 4 11 0 21 36 Total 0 0 0 48 121 0 180 349 I Total 0 0 0 51 126 0 187 364 I Total 0 0 0 36 112 0 149 297 Total 0 0 0 45 121 0 192 358 File: sqn101_050311data.xlsx Entered by: J. Jner Reviewed by.

  • * * \.....) Environmental Testing Solutions, Inc. Co ncentration "I 2 3 Co ntrol-J 28 28 3 1 33 30 34 33 32 3 1 36 32 34 86.4*/. 34 35 33 100% 38 36 36 Control-2 29 26 34 J lata.kc 37 37 35 Outran JOI: OUBnett's 1\tSD va lu e: 1.698 PMSD: 5.7 latakt: OUBnett's MSD va lue: 1.569 PMSO: TV A I Sequoyah Nuclear Outfall lOt Non-treated May 03-10, 2011 Ceriodaplmia dubia Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control --*L--, ,..., _

.. __., Verification of Data Entry, Calculation s , and Statistical Analyses Proj ect numbe.r: 7 068 Re_11l i cate aumbe.r S urvival Average re productio.n Cocllic:ioa t of P HUD I red*ctloto 11om 4 5 6 7 8 9 J O (*/e) (offs:p riagl remat e) variatioa (%) -...a(%1 31 3 1 31 30 29 29 29 100 29.7 4.2 N ot a ppli ca b le 32 27 33 30 28 30 32 100 30.9 7.4 -4.0 36 29 32 3 1 31 31 33 100 31.9 5.8 -7.4 30 35 36 35 34 32 34 100 33.8 5.7 -13.8 35 36 37 36 35 33 35 tOO 34.9 3.7 *1 7.5 37 35 38 36 36 36 36 100 36.4 2.7 -22.6 30 29 27 30 32 31 29 100 2 9.7 7.8 No t applicable 35 37 35 32 36 38 36 1 00 35.8 4.7 -2 0.5 MS D = Minimwn Signi fi cant Difference PM S D = P ercen t Minim u m Significant Differ e nce P M SD is a measure of test precisiOIL The P MSD is the mini mum percent di fference bern-een the oo n trol and treatment that can be declared statistically significant in a whole effiuentto.xic i ty tesL l.ov.'ef" PMSD bound determined by USEPA (Ia-percen ti le) = IJ'Yo. Upper P MSD bound detennined by US percentile)= 47%. Lower and upper P M SD bounds were determined from the I Oth and 90th percen t i l e, respectiv e ly , of P M SD data from EPA's WET lnterlaboratOJy Variability S t udy (USEPA, 200 1 a; USEPA. 2001b). USEPA. 2000. Understan d ing and Accoun t ing f or M e!hod Variability in Whole Effluent Toxici ty A pplications Under the Nationa l Pollutant Discharge E li mination Pr ogram. EPA-833-R-()().()()3. US Environmen tal Pro t ectio n Agency , Cincinnati, OH. US EPA. 200la. 200 I b. Fi nal R epon: llltcrlaboratoty Variabi l ity S t udy of E PA Short-term Otron i c and A cute W hole Effluent Toxicity Test M ethods. Vo l umes I and 2-Appendix. EPA-821-B-0 1-004 and EP A-821-B-0 1..005. US Environmen tal Protection A gency , Cincinnati , OH. Pag e 47 of 100 Fil e: sqn101_050 3 11d a ta.xlsx T able populated fro m associ ated "Veri fi ca ti o n of Ce ri odaphnia Reprod ucti on To ta ls" s pread s h ee L Sp readsheet en ter e d by: J. Sud\r Rev i ewed by: I,,,._,_., TVA I Sequoyah Nuclear Plant, Outfa11101 Non-treated .ETS * *

  • 0 ..
-**. Environmental Testing Solutions, Inc. Start Date: 5/3/2011 End Date: 5/10/2011 Sample Date: Conunents:

Cone-% 2 Control-I 28.000 28.000 Control-2 29.000 26.000 10.8 33.000 30.000 21.6 33.000 32.000 43.2 36.000 32.000 86.4 34.000 35.000 100 38.000 36.000 Intake 37.000 37.000 Cone-% Mean N-Mean Control-I 29.700 1.0000 Control-2 29.700 1.0000 10.8 30.900 1.0404 21.6 31.900 1.0741 43.2 33.800 1.1380 86.4 34.900 1.1751 100 36.400 1.2256 Intake 35.800 1.2054 Auxiliary Tests TestlD: LablD: Protocol: 3 31.000 34.000 34.000 31.000 34.000 33.000 36.000 35.000 Mean 29.700 29.700 30.900 31.900 33.800 34.900 36.400 35.800 Kolmogorov D Test indicates normal distribution (p > 0.0 I) Bartlett's Test indicates equal variances (p = 0.13) The control means are not significantly different (p = 1.00) Hypothesis Test (1-tail, 0.05) NOEC Dwmett's Test 100 Treatments vs Control-I May 03-10,2011 Statistical Analyses Ceriodaphnia Swvival and Reproduction Test-Reproduction CdFRCR Sample 10: ETS-Envir. Testing Sol. Sample Type: FWCHR-EPA-821-R-02-013 Test Species: 4 5 6 31.000 31.000 31.000 30.000 29.000 27.000 32.000 27.000 33.000 36.000 29.000 32.000 30.000 35.000 36.000 35.000 36.000 37.000 37.000 35.000 38.000 35.000 37.000 35.000 Transform: Untransformed Min Max CV% 28.000 31.000 4.214 26.000 34.000 7.784 27.000 34.000 7.388 29.000 36.000 5.809 30.000 36.000 5.717 33.000 37.000 3.687 35.000 38.000 2.654 32.000 38.000 4.711 7 30.000 30.000 30.000 31.000 35.000 36.000 36.000 32.000 N 10 10 10 10 10 10 10 10 Statistic 0.829582155 8.592660904 0 -TV A I Sequoyah Nuclear Plant, Outfall 101 DMR-Discharge Monitoring Report CD-Ceriodaphnia dubia 8 29.000 32.000 28.000 31.000 34.000 35.000 36.000 36.000 9 29.000 31.000 30.000 31.000 32.000 33.000 36.000 38.000 !-Tailed t-Stat Critical * -1.616 -2.963 -5.523 -7.005 -9.025 2.287 2.287 2.287 2.287 2.287 Critical 1.035 15.08631706 2.100923666 10 29.000 29.000 32.000 33.000 34.000 35.000 36.000 36.000 MSD 1.698 1.698 1.698 1.698 1.698 Isotonic Mean N-Mean 32.933 1.0000 32.933 1.0000 32.933 1.0000 32.933 1.0000 32.933 1.0000 32.933 1.0000 Skew Kurt -0.138685175 0.300894097 LOEC ChV TU MSDu MSDp MSB MSE F-Prob df >100 1.697549464 0.057156548 64.58666667 2.755555556 1.9E-12 5,54 Point % so 95%CL Linear Interpolation (200 Resamples) Skew ICOS >100 IC10 >100 !CIS >100 IC20 >100 IIC25 >tool IC40 >100 IC50 >100 Page 48 of 100 File: sqnl01_05031ldata.xlsx Entered by: J. Sumner Reviewed by: J{ o.ol " " TV A I Sequoyah Nuclear Plant, Outfall 101 -Intake Non-treated .ETS * * * * ,.... . u . *" -:> Environmenta l Testing Solutions, Inc. Start Date: 5/3 1201 I Tes t iD: End D ate: 5110120 11 LablD: Sample Date: Protocol: Comments: Cone-% I 2 3 Control-I 28.000 28.000 31.000 Control-2 29.000 26.000 34.000 10.8 33.000 30.000 34.000 21.6 33.000 32.000 31.000 43.2 36.000 32.000 34.000 86.4 34.000 35.000 33.000 100 38.000 36.000 36.000 lntak e 37.000 37.000 35.000 Cone-% Mean N-Mean Mean Contr ol-I 29.700 1.0000 29.700 Control-2 29.700 1.000 0 29.700 1 0.8 30.900 1.0404 30.900 2 1.6 31.900 1.0741 31.900 43.2 33.800 I.I380 33.800 86.4 34.900 1.1751 34.900 1 00 36.400 1.2256 36.400 Intake 35.800 1.2 054 35.800 Auxiliary Tests Shapiro-Wilk 's Test indicates normal distribution (p > 0.01) F-Test indicates equal variances (p = 036) The control means are not significantly different (p = 1.00) Hypolhesis Test ( 1-tail , 0.05) Homo sce da.stic t Test indicates no sigoiiiCllot diffenoces Tnatmeots vs Cootrol-2 Page 49 of 1 00 May 03-10, 2011 Statistical Analyses Ceriodaphnia Survival and Test-R eprodu ctio n CdFRCR Sample ID: ETS-Envi r. Testing Sol. Sample Type: FWCHR-EPA-82 1-R-0 2-01 3 Test Species: 4 5 6 7 8 31.000 31.000 31.000 30.000 29.000 3 0.000 29.000 27.000 30.000 3 2.000 3 2.000 27.000 33.000 30.000 28.000 36.000 29.000 32.000 31.000 31.000 30.000 35.000 36.000 35.000 34.000 35.000 36.000 37.000 36.000 35.000 37.000 35.000 38.000 36.000 36.000 35.000 37.000 35.000 32.000 36.000 Transform: Untransformed Min Max CV% N t-Stat 28.000 31.000 4.214 10 26.000 34.000 7.784 10 27.000 34.000 7.388 10 29.000 36.000 5.809 10 30.000 36.000 5.717 10 33.000 37.000 3.687 10 35.000 3 8.000 2.654 10 32.000 38.000 4.711 10 -6.741 Statistic 0.949571669 1.87890625 0 MSDu MSD p 1.569196635 0.052834904 TV A I Sequoyah N u clear Plant, Outfall I 0 I -Intak e DMR-Discbarge Monitoring Report CD-Ceriodaphnia dubia 9 29.000 31.000 3 0.000 31.000 32.000 33.000 36.000 38.000 1-Tailed Critical 1.734 Critical 0.868 6.54I085n 2.1 00923666 MSB 186.05 10 29.000 29.000 32.000 33.000 34.000 35.000 36.000 36.000 MSD 1.569 Skew Kurt -O.t3n86 t 5 0.558140392 MS E F-Prob df 4.094444444 2.6E-06 1, 18 file: sqnl0 1_0503 1ld ata.xlsx E ntered by: J. Sumner Reviewed by: J'\

  • * *
  • u E . s Environmental Testing Solutions, Inc. Coneentration Control 10.8% 22.6% 43.2% 86.4% 100% 100% Intake Page 50 of 1 00 I. TV A I Sequoyah Nuclear Plant, Outfall101 -Non-treated May 03-10, 2011 Ceriodaphnia dubia Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses File: sqn101_050311chem.xls Entered by: s;rns Reviewed by: 7068 I I I I I I I I I I I I I I ' Species: Ceriodapltnia dubia Client: TVA I Seguoyah Nuclear P l ant, OutfalllOl CONTROL 10.8% 21.6% 43.2% 86.4% tOO% 100% Intake Page 51 of 100 Page 6 of7 Date: OS*<S'b*\\

SOP AT II -Exhibit AT 11.2, revision 04-01-09 I I. I; l i l l I I I* I I I' I I ' f I CONTROL 10.8% 21.6% 43.2% 86.4% 100% 100% Intake .) Species: Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant, OutfalllOl Parameter Page 52 of 1 00 '"""" Page 7 of7 SOP A Til -Exhibit AT II .2 , revision 04-0 1-09 I j I I I : I I I -I I I I I I I I I l l . I I *eTS

  • 0 0 * ) .... . . : ... Pagel of6 .) Envlronmt:ntaiTestlng Soludon5.

I nc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Seguoyah Nuclear Plant NPDES #: .. Ot>'2J.'-\'SO Project #: 1o<..8 Dilution preparation information: Dilution prep (%) 10.8 21.6 43.2 Effluent volume (mL) 270 540 1080 Diluent volume (mL) 2230 1960 1420 Total volume (mL) 2500 2500 2500 Test O'f!anism information: 86.4 2160 340 2500 Organism age: .,\ "\'i "-'oJJlf:. Date and times organisms 05-0"t*\\

  • '-00 were born between: Organism source: lttml'L oc;-cn. .. l \ Transfer bowl information:

pH= "'\,CD& s.u. Temperature .= "\.".'1.... oc Average transfer volume: c. Daily feeding and renewal information: 100 2500 0 2500 t-\N*W.:r"ON Outfall: 101 Comments: Each concentration was UV -treated for 2 minutes to remove pathogenic Interferences. Test information: Randomizing template: 6\.ve. Incubator number and .3& shelf location: Artemia CHM number: Drying information for weight determination: Date I Time in oven: Initial oven temperature: loti'(. Date I Time out of oven: OS*\\* \\ Final oven temperature: Coc'<.. Total drying time: 1.'l* .. Day Date Morning feeding Afternoon feeding Samp l e numbers used batch 0 2 3 4 5 6 7 Page 53 of 1 00 SOP A T20-Exhibit A T20.3 , revision 04-01-09

I I I I I I I I I I I I I I I I I I I I *eys * * * . : . . ." '..... . . .*. ; !....,___ .* ,;;! .*. Page 2 of6 .) EnvlranmantaiTestlns So!udons, Inc. Species: Pimephales promelqs Client: TVA I Seguoyab Nuclear Plant, OutfalllOl. UV-treated Date: OS*<Ai*t\ ' Survival Data Day 10.8%_ 21.6% A B c; Q E 1' G H _[_ J 0 /I) /C IO 10 *IC /C) ID ID IO /C 1 (Q 10 10 10 10 /0 /0 lO 10 rc 2 10 10 ro ro 10 10 /0 IO 10 IO 3 to fQ 10 ft;, It) 10 IC IQ IO IC 4 It> 10 /0 'O ro IO /Q IO IO to s to IO IO 1o 10 ID to /0 /Q /Q 6 to tO to /0 16 10 I() IO IO tO 7 lo" IO 10 IO 1o IO IC IO IO A = Pan weight (mg) Tray color code:: tf.Ol 14.91 14-.(,& t4.'i>!. Analyst: rv'l Date: 8 =Pan+ Larvae weight (mg) Analyst: Mltl [c..r. " t.t..t.'l lt.t.lt. !t..t*Sl lv:i-.lo ltJ1.'1 lt13.G,4 ?,l,'f"' Date: OS* II C =Larvae weight (mg) = Hand ,*'"' C\,o& .. '""'" ,,,l t.fo, -t;tfC &.'\ .. Analyst: AI-- Weight per initial number of larvae (mg) = C I Initial number of larvae Hand o" .).."' r,..o'-'rat.\ '\o . o.;: " Analyst: . -()* o* o* o* b' b' ()" JO Average weight per Percent reduction o.l<-1 initial number of from control(%) larvae(me) . , .. Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: K L 10 10 10 .10 IO 10 10 10 IO (0 10 10 10 IO IO to \;.\(, t;.\ol Z.0-1" iu.JtJ 1,.,4\ t.c; "'o.,_ o.t7. Page 54 of 1 00 SOP AT20-Exhibit AT20.3, revision 04-01-09 ""' I l I ' ' ' I I I I I -I I I I I I I I I I -I I .ETS * . ' . -j :. ' ).'"**.* .** ..) EnvlronmenuoiTastlng Solutions, Inc. Page 3 of6 Species: Pimeohales oromelas Client: TV A I Seguoyah Nuclear Plant, Outfa11101. UV-treated Survival and Growth Data Day 43.2% 86.4% 100% M N 0 p Q R s T u v 0 /() ID IO ID /D -10 (0 IC 10 IO 1 /0 ID fD /{;) I(J /0 ID lO IO 10 2 (0 10 I() 10 10 tO to (() IO to 3 /0 /t) fO I() ID ID IO lC /C IC 4 10 tO lO 10 fO to IO 10 10 IO 5 lO f() lO 1'4 tD 'I'A I() to 10 10 6 /0 10 10 '\. 16 10 10 10 10 7 lD tO ((J ' ((J 1\ \0 10 to tc A= Pan weight (mg) M!mfa,!!fl Tray color code:: 14*1'-Analyst: frll\tC 14.4z, 14-?A 14.2k t+.c.-1 lt-.;') 1\.1\C.. Date: B =Pan+ Larvae (mg) Analyst: M t.?>.SS t,z..ti[f t.'L.It. u.:u !:L.'U z.z.."' u.,, r't.'la Date: C = Larvae weight (mg) = B-A Hand calculated(!}._ t.-t\ c:n. &.'4, t.So ,,,b ,,,1.\ l"l*C\' t.ll. ** Q1. Analyst: "'-Weight per initial number of larvae (mg) = C I Initial number oflarvae c.:,O .,,'V ... Hand calculated. ,. . ,; fl* Analyst: -o* C)* C)' t)* o* o* o* ()' Average weight per Percent reduction initial number of from control(%)

  • o.t7.

'(. 01. o:t&o larvae (me) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: w X If;) fO IC ./D to IO /0 'l rc( IO 'l I() q IQ 1 \St'\ 4ll tc \lr.?lo u.4? c..C.b Q.t.fb '!oo .>,.0 " \ o* . c.. '1. 7 .. Page 55 of 1 00 SOP AT20-Exhibit AT20.3, revision 04-01-09 i I I ) Env i ronment a l T esting So l ution s. I n c:. Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant., OutfalllOl, UV-treated Day 0 2 3 4 5 6 7 A= Pan weight (mg) T r ay co l o r code:: M'/l:uh'\flr Analy s t: M \!:C Date: oC:.,-o4.\\ B =Pan + Larvae weight (mg) Analyst: MM Date: 012*l't* n C = Larvae weight (mg) = B-A Hand Analyst: 61.,. Weight per initial number of larvae (mg) = C I Initial number of larvae Hand calculated. Analyst: Average weight per Percent reduction initial number of from control(%) /0 IO 10 to tO f() to I() ({) ID IO IO 'O ID 10 lO Comment codes: c = clear , d = dead , fg = fungus , k = killed , m = missing, s k = s ick , sm = unusually small , lg = unusually large , d&r = decanted and returned , w = wounded. Comments: Page 4 of6 Page 56 of 1 00 SOP A T20-Exhibit A T20.3, revision 04-01-09

  • * * * (] Environmental Testing Solutions, Inc. c-.......SO.

(%) Coelrol 10..1% 21.6% 43.2% 86..4% 100% * .,... i*taiLe Ouo r.o 101* Do u o-t'a MS D v atu: P MSD: D UIAdt'aMSDYllfu o: PM SD: ......... A B c D £ F c H I J K L M N 0 p 0 R s T u v w X y 7. M B B bi4&l *UIIJtt fll ....... 10 10 10 10 10 10 10 10 10 10 1 0 1 0 1 0 10 1 0 1 0 1 0 1 0 1 0 10 10 10 10 10 10 10 10 10 1 2..1 0.1 014 12.3 l""lalll e...a.:tartuTar 10 10 10 10 10 10 10 10 10 10 10 10 10 1 0 1 0 9 1 0 9 1 0 1 0 1 0 10 1 0 9 10 10 10 9 A *l'u .apt (OOC) 14.07 1319 12.59 13.J8 1509 1335 IJ.24 14.87 14.68 14.06 13.16 13.1 4 14.76 14.42 1 3.76 14.39 1383 1 5.23 1 426 14.64 1 2.55 11.96 14.80 1485 13.26 1384 13 58 1 4.80 M.S D* PMSD* TV A I Sequoyah Nuclear Plant, Outfall 101 UV-treated Ma y 03-10, 2011 Pimephales pronrelas C hronk Whole E fflu ent Toxic ity Tes t E PA-821-R-02-013, Me thod 10 0 0.0 Quality C ontrol Ve rification of Data E ntry , C alculatio ns , and Sta t istic a l A naly ses Not for CamplilllCC ln l<mal L.abcnllory QC ...... ................. (OOC) Mou ...... l Cadlic:kal gf nriaJioa \l'd&kf J laldaJ au.lln" wdpi (OOC) *A*B ...... ., ........ <aoc> kn'trl..&*.-btt"d 0'-........ ---. Cllo.n...(OOC) o.n .. t"l ...... ., ..... (%) 2121 7 14 0.7 14 0.714 22.27 9.08 0.131 9.9 0.908 21.12 8.5.1 0.8SJ O.S SJ 2 18 7 8.49 0.349 0.84 9 2410 9.01 0.90 1 Q901 2 1 48 8.13 0.8 1 3 0.1167 4.4 0.813 2 1 91 8_67 0.867 0.867 23.n s.ss 0.88S 0.88 5 23.64 8.96 0.896 0.896 2 1.99 7.93 0.793 082 4 6.J 0.79 3 20.95 7.79 0.779 0.779 21.43 8.29 0.829 0.829 23 55 8.79 0.879 0.879 22.94 8.52 0.8 52 0.859 1.6 0..152 22..23 8.47 0847 0.84 7 22.12 7.73 0.859 o.m 22.33 8.50 O.SSO 0.850 2293 1.70 0.8 56 0819 4.9 o.no 22.00 7.74 O.TI4 OTI4 22.61 7.97 07'Y/ 0.'197 20.71 8.1 6 0 81 6 0.8 16 1 99 8 8 02 0802 0803 139 0.802 2 1.40 6.60 0.660 0.660 2325 8.40 0.93) 0.840 22.74 9.4 8 0.948 0.948 22.67 883 0883 09 11 34 0.88 3 22.83 9.25 0_925 0.925 22.79 7.99 0_888 0799 M mmu n Sopoanl Difl'c<CDCC Percent M munum s;p;r_,. DUI'cn:nce 7068 __ .. llkuwdpl/lailbl Codl1dnlfll -.... ..-.... (%) ...... fllilnat v.attaato. .,._.....,. -(%) IOOCl ,. ...... "" 1 00.0 0,.13 1 9.9 N o< applicable 100.0 0..167 4.4 -0 10(1.0 G.8l4 6.3 0,.1 91.5 0.838 5.4 ..... 91.5 0.791 4.6 4.0 91.5 0.7 80 10.4 6.2 91.5 0..8811 7.4 -6.9 P MS D tS a III<:OSift of t esz precmon. Tbe P MS D os ohe minimum perc:eot dif!'..-..- .......,_, ohe OCIIIIfOI and tn:a<men1 lhat can be clcdarcd ............Oy "' a ¥oilole cftlu<na ooxicity lest. Lov.--.r P MS D bouDd dcunniaed by USE!' A (I Doh pera:olile)

  • 1 2% U-PM S D bculd cleoanu oed by U SEPA (900h percalllle)
  • 30%. Lowu mel upper P MS O bound. won: ddennmed from the IOOh mel 900h potea>lile.

mpeajvdy. o f PMSD daa from EP A's W ET V arilloilily Study (U S EPA, 1001 a; U SE.PA, lOO I b). USEPA. 2001a. 200 1 b F ioul Rcpon* lnurlabonuory VariabllityS.udyof EPA Sbon*lerm Oa-onicand Aaa e Wbol e EftlOCD tT o xicity Test M elbods. Volumes I mci2-Appc:adox. and EPA-82 1-8-0 I.oo5. US En n fOfl-l'roooctJoa ,._, CiDCUIDali. OH.. Page 5 7 of 1 00 Fie: sqn 1 01 050311cfa1a-uv.xlsx J.Sumner Reviewed l>)r. c:J\- ! TS TV A I Sequoyah Nuclear Plant, Outfall101 UV-treated May 03-10, 2011 Statistical Analyses \) Environmental Testing Solutions, Inc. Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/3/2011 Test ID: PpFRCR Sample ID: End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: Comments: Cone-% D-Control 0.7140 10.8 0.9010 21.6 0.8960 43.2 0.8790 86.4 0.8500 100 0.8160 Intake 0.9480 2 0.9080 0.8130. 0.7930 0.8520 0.7700 0.8020 0.8830 3 0.8530 0.8670 0.7790 0.8470 0.7740 0.6600 0.9250 4 0.8490 0.8850 0.8290 0.7730 0.7970 0.8400 0.7990 Transfonn: Untransfonned Cone-% Mean N-Mean Mean D-Control 0.8310 1.0000 0.8310 10.8 0.8665 1.0427 0.8665 21.6 0.8243 0.9919 0.8243 43.2 0.8378 1.0081 0.8378 86.4 0.7978 0.9600 0.7978 100 0.7795 0.9380 0.7795 Intake 0.8888 1.0695 0.8888 Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.01) Bartlett's Test indicates equal variances (p = 0.63) Min 0.7140 0.8130 0.7790 0.7730 0.7700 0.6600 0.7990 Hypothesis Test (1-tail, 0.05) NOEC LOEC Dunnett's Test 100 >100 Treatments vs D-Control Point % SD 95%CL(Exp) IC05 75.275 ICIO >100 IC15 >100 IC20 >100 IIC2S >1001 IC40 >100 IC50 Page 58100 Max CV% N 0.9080 9.930 4 0.9010 4.417 4 0.8960 6.341 4 0.8790 5.419 4 0.8500 4.614 4 0.8400 10.417 4 0.9480 7.382 4 Statistic 0.93555665 3.45981002 ChV TU MSDu 0.10085134 Linear lnterpoJation (200 Resamples) Skew t-Stat -0.848 0.161 -0.161 0.795 1.231 MSDp 0.12136142 TV A I Sequoyah Nuclear Plant, OutfalllOl DMR-Discharge Monitoring Report PP-Pimephales promelas 1-Tailed Critical MSD 2.410 0.1009 2.410 0.1009 2.410 0.1009 2.410 0.1009 2.410 0.1009 Critical 0.884 15.0863171 MSB MSE 0.00376394 0.00350235 Isotonic Mean N-Mean 0.8488 1.0000 0.8488 1.0000 0.8310 0.9791 0.8310 0.9791 0.7978 0.9399 0.7795 0.9184 Skew Kurt -0.827227683 0.41674318 F-Prob df 0.406950474 5, 18 File: sqnl01_0503lldata-uv.xlsx Entered by: J. Sumner Reviewed by: --b- .1. *'* __ ....... .,, *-* '******--*-***-

  • ,,j ___ .._ ........

... ,,....., .. . .,s..**_,,.,.., :> .* -.---..... ........ TV A I Sequoyah Nuclear Plant, Outfall 101 -Intake UV-treated Start Date: 5/3/20 II Test ID: End Date: 5/10/2011 LabiD: Sample Date: Protocol: Comments: Non-treated Cone-% 1 2 3 D-Control 0.7140 0.9080 0.8530 10.8 0.9010 0.8130 0.8670 21.6 0.8960 0.7930 0.7790 43.2 0.8790 0.8520 0.8470 86.4 0.8500 0.7700 0.7740 100 0.8160 0.8020 0.6600 Intake 0.9480 0.8830 0.9250 Cone-% Mean N-Mean Mean D-Control 0.8310 1.0000 0.8310 10.8 0.8665 1.0427 0.8665 21.6 0.8243 0.9919 0.8243 43.2 0.8378 1.0081 0.8378 86.4 0.7978 0.9600 0.7978 100 0.7795 0.9380 0.7795 Intake 0.8888 1.0695 0.8888 Auxiliary Tests May 03-10,2011 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth PpFRCR Sample ID: ETS-Envir. Testing Sol. Sample Type: FWCHR-EPA-821-R-02-013 Test Species: 4 0.8490 0.8850 0.8290 0.7730 0.7970 0.8400 0.7990 Transform: Untransformed Min Max CV% 0.7140 0.9080 9.930 0.8130 0.9010 4.417 0.7790 0.8960 6.341 0.7730 0.8790 5.419 0.7700 0.8500 4.614 0.6600 0.8400 10.417 0.7990 0.9480 7.382 N 4 4 4 4 4 4 4 Statistic t-Stat -1.096 Shapiro-Wilk's Test indicates normal distribution (p > 0.0 I) 0.88838106 F-Test indicates equal variances (p = 0.72) 1.58184743 Hypothesis Test (1-tail, 0.05) MSDu MSDp Homoscedastic t Test indicates no significant differences 0.10242295 0.12325264 Treatments vs D-Control Page 59 of 1 00 TV A I Sequoyah Nuclear Plant, Outfall IOJ DMR-Discharge Monitoring Report PP-Pimephales promelas 1-Tailed Critical 1.943 Critical 0.749 47.4683456 MSB 0.00667012 MSD 0.1024 MSE 0.00555646 Skew Kurt -0.9249599 -0.3380108 F-Prob df 0.31525472 1, 6 File: sqnl01_0503lldata-uv.xlsx

  • Entered by: J. Sumner Reviewed by: --tr-Environmental Testing Solutions, Inc. Concentration Control 10.8% 21.6% 43.2% 86.4% 100% Page 60 of 1 00 ;. ;, ,; ; ;j, i-l TVA I Sequoyah Nuclear Plant, OutfalllOl

-UV-treated May 03-10,2011 Pimephales promelas Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses File: sqn101_050311chem-UV.xls Entered by: S:Jfns Reviewed by: l I I . I . I I I I I I I I I I .. I I .. ) ' ,, ' s 'f. ;: ,.._; > ) EnvfronmtntaiTesting Sofullons. l nc. Page 5 of6 Species: Pimephales promelas Date: OS*<e>-\\ Client: TVA I Seguoyah Nuclear P l ant, OutfalllOl, UV-treated CONTROL UV-treatcd 10.8% 21.6% 43.2% 86.4% 100% 100% Intake Page 61 of 100 SOP AT20-Exhibit AT20.3 , revision 04-01-09

.)!=T. . ..) Env fro nmenu d Tenlng Solutions , I nc;, 'I I CONTROL I 1 10.8% I I I I I I .I : I I --I I 21.6% 43.2% 86.4% 100% 100% lntake Page 62 of 100 (4to)llN<.' Page 6 of 6 S OP AT20-Exhibit A T20.3, revision 04-01-09 l i I I I I I I ' : t I '* r-> .) EnvlronmentaiTestlng Soluttons,lnc.

Date analyzed 0S:3 II L b I d d a oratory contro stan ar : Reference standard number lMssqa 1 Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = O.LO mg/L Meter: Accumet Model AR25 pH/Ion Meter True value (TV) Measured va lue (MV) (mg/L) (mg/L) 0.50 0.'-(S) Sample characteristics Iodide 1--J _ _, Acid reagent:! L... --..!.\ % RS = MV I TV x 100 (acceptable range= 90 to 110%) tlo.a.. *t. Sample ID Sample characteristics Note: All samples were analyzed in excess of EPA recommended holding 5 minutes) unless o therwise noted. L b t t I d d a ora orv co11 ro stan ar : Reference standard True value (TV) Measu red value (MV) % RS-MV /TV x 100 number (mg/L) (mg/L) (acceptable range= 90 to llO%) \ N.SS qz., 0.50 ().'{Sl, 9D. C/'/. Reviewed by Date reviewed 0 S

  • I\ Page 63 of 1 00 SOP C8 -Exhibit C8. I , revision 09-0 t-09
J I ' I I j
:
  • . . . ., ' ' . ) . ; . * !-----' .) Environmental Te.stlng Solutions, Inc. Total Residual Chlorine (Orion Ele ctrode Method, Orion 97-70) Matrix: Water, JU, =*o.IO mg/L Page tJ1-Page _l_ of_l_ Analyst J=-----i Date analyzed 06 .0 6 .11 Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent: r\ Acid reagent: . ( C\112-Y iJb L b I d d a o rato ry cont ro s tan ar : Refe renc e sta nd ard True va lu e (TV) Meas ur ed va l ue (MV) % RS = MV I TV x 100 number (m g/L) (mg!L) (acceptable range= 90 to 11 0%) \l\JSSC1 1.\ 0.50 10\Q Samp l e c h a rac teristics Sample characte ri stics 2.06(, No te: All samples were analyzed in excess of EPA recommended holding time (IS minutes) unless noted . .1 L b I d d -i.

..J:r a oratory co11t r o stall ar : Reference sta nd a r d True value (TV) Measu r ed value (MV) % RS =MY I TV x 100 numb er (mg!L) (mg!L) (acceptab l e range= 90 to 110%) \NSSqvt 0.50 .O'liJ Reviewed by I Date reviewed a= OS*\\ Page 64 of 1 00 SOP C& -Exhibit C&.l , revision 09-01-09 --"" *,-th 1*":1 I 1 1 -1. ,., I , .ETS if', a.: : .. *. Environmental Testing Solutions, Inc. Analyst rlYllif.-; Date analyzed ()?;;;; . \l L h I d d a oratory contro stan ar : Tota l Residual Chlorine Page '10 Page_\ _ of_l_ (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter [odide reagent: Acid reagent: . Reference standard True va lu e (TV) Measured value (MV) % RS = MV I TV x 100 numb er {mg/L) {mg/L) (acceptable range= 90 to 110%) \ 0.50 0.'54&\. t[)Gt.&1., Sample characteristics Sample lD Samp l e characteristics Note: All samples were analyzed in excess of EPA recommended holding time (IS minutes) unless otherwise noted. L h l a oratory contro stan d d ar : Reference standard number ""LN.) S '\'UI Page 65 of 1 00 True val u e (TV) Measured value (MV) (mg/L) {mg/L) 0.50 o. '50() % RS = MV I TV x 100 (acceptab l e range= 90 to 110%) IOO.o*l, Reviewed by Date reviewed .... L..:..*....;l ..... l _ __, SOP C8-Exhibit C8.1, revision 09-01-09 I -I ., ; ) ;"' .,. i:o* .-. "**'" .. ..) ) : "---" ...... ---.J J £nvlronment.afTe .stfng So lutions,. fnc. Analyst __ --ll Date analyzed D5-05-1l l Alkalinity (SM 2320 B) Matrix: Water , RL = LO mg CaC0 3/L Titrate samples to pH=4.5 S.U. Page ___!__.of_4_* __ Time initiated I j D5o Time completed determination: Begin End Total ml ml ml .(.? End \ Duplicate (B) D.D 'k Matrix spt e recovery: Reference standard Spike va lu e Sample number (SV) volume Begin End (ml) ml ml ::rNSS too o.o -:J-.IP Normality (N) of H 2 S04 = (5 ml NazC03 x 0.05)/E =0.25/E = 0.0180-pH Factor or Multiplier = (N x 50000)/ 100 ml sample =Nx 500 Alkalinity (MV) % RS= MV /TV x 100 (acceptable range =90 to LIO%) Total ml 3.1 Total ml -=1-.\R Mu ltiplier (mg CaCOJIL) Multiplier Spike alkalinity (A) Multiplier (mg CaCO)!L) C!.q '=t5 Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaCO)I'L) = 75 tol25%) 31 s l ample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) m l ml ml Multiplier (me CaC031L) DSL"h.U \DO 13.$ 5.9 Cf.q ,..,. .... :{' M as <n*tiA 16.5 IC!.5 l.?.O sq t:::i>tC '" iiodl-d.B \<l5" ;),5.<:; lo.o 5a . tJq._z.l, I) 31.5 Co.o S'j I\ 3\.5' 31R (Q.3 ltZ... \A l3l.g 5'1 :;,. to.n CJ.1 to.o (.p.O Sti SsvJ ' 1'5*2.. 3.2. ---3'2. Reviewed by: Date reviewed: !0 5 -{)'t *{ l Page 66 of 1 00 SOP C6-Exhibit C6.1 , revision 09-0 l-09 I I I I I ) £nvlton mt nt*l Tes t ing I nc. Analyst Date analyzed l>5* 08. 11 Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC0 3/L Titrate samples to pH == 4.5 S. U. Page l.f-\ Page Time initiated I i Time completed _ Titrant normality and multiplier determination: J!.R of Norma lity Deionized Titrant c ... ,. ..... D End water reference standard ml ml =4.5S.U. number number L b a oratory co11trol sta11dard: Reference standard True value Sample number (TV) volume Begin (mg CaCOy'L) (m l) ml 100 100 )5.2--a r1X sp 1 e recovery: Reference standard Sp i ke value Sample number (SV) volume Begin (mg CaCOJIL) (ml) ml .1"NS5 5'0 1'0 0 3?>.1-Total 1111 _(_E) . End ml :::> End m l 1m Normality (N) of H 2 S0 4 pH Factor or Multiplier (5 m l Na 2 C0 3 x 0.05)/E = (N x 50000)/ 100 ml sa mp l e 0.25/E (acceptab le range-_ v. Total Multiplier m l G)_) C\.1 M u ltiplier Total Multip lier ml 1k/_ q_q =NxSOO 11 nno) ---;--. .. Alkalinity (MV) % RS = MV I TV x 100 (mg CaC{h/L) (acce ptable range =90 to 110%) C,o Alkali n ity (mg CaCOJIL) s q D qo.o 0 lo %RPD= {(S-0)/[(S+D)/21} x 100 (acceptable range=:!: 10%) Spike alkalinity (A) (mg CaCOJIL) IJ-t O Sample alkalinity (B) Measured sp ike value (MV) % R = MV I SV " 100 (mgCnCOYL) MV=A-B (acceptab le rang e _(_mgCaCOy'L) =75 to 125%) r+-=t .... s l amp1e measurements: Sample volume Begin End Tota l Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mgCaCOYLl LOO o.o Cl.IP q_q qs <1)S.OZ-liA I q ,\p '2.1.1.1 120 lDaJl Cu.L l 21.1P (1.4-liD II 0 'Sb

  • 0"2-\ 7__ 3:3-0 I J-14 J \\.i II DSIS1. 01-I 3 D.G ,,_ 1 II.) )10 II CS1-Ct.r()O"()...

J @) ll.l ct.S r21 \qo 11 OSbC::.* (J1__ .. t Ju:(..... 3\.VJ 11.0 2?...() II ac; tn. ol 3 3_i.(o llfD I I \ -t\f>P6l. 71-0 II OS0'2..o \ -1'"v A ;-s & 'II) lt\l } '0 .\) Revtewed by: Date r e viewed: j <::l5-V'i*ll Page 67 of 1 00 SOP C6 -Exhibit C6.l , revision 09-0 l-09 I z j j l I ; ! -I -}. ' ' I] (J [1 -r' .. 0; 3 .. . . ;---' ) f.nv f ronmont*IT*st J ng Solut i on s. In c. Analyst I Bst-Date analyzed ,-, -----i Alkalinity (SM 2320 B) Matrix: Wa t er , RL = 1.0 mg CaC0 3/L Titrate samples to pH=4.5S.U. Page 3 of_!+/-____ Time initiated§:: Time completed c:=

  • ttrant normality and mult i plier determination:

nil .l' Nnrn111 1i tv Deionized Titrant check water reference standard = 4.5 s.u. number number L b t I d d a ora ory contro stan ar : Reference standard True value number (TV) (mg CaCOYLJ 100 Sample ID z. M4 k atnx spi e recovery: Reference sta n dard Spike va l ue number (SV) (mgC a COYLJ \00 Begin ml Sample volume (ml) 100 Sample volume Sample volume (ml) 5o Sample alkalinity (B) (mg C aCOVL) 5+-s l amp, e measurements: t:na ml Begin ml ."1-Begin ml l-4-.4 l u .... ml (E) End ml 1\.q End End m l Normality (N) of H 2 S0 4 pH Factor o r Multiplier ' '" ml Na 2 C0 3 x 0.05)/E -;;;,t..l.::ll"' = (N x 50000)1100 m l sample (acceptable range-0.0180-0.0220) 500 --Total ml q.'2. Total m l :J.lR Alkali ni ty (MV) % RS = MY I TV x 100 Multiplier (mgCaCO YL) (acceptable range =90 to 110%) '1.4 C\1 GJI D ... . ' . !C".c_ Alkalinity %RPD= Multiplier (mg CaCOYL) {(S-D) /[(S+D)/21} X 100 (acceptable range= :1: 10%) Spike alkalinity (A) Multiplier (mg CaCOYL) iz\t1.CJ )50 Measured spike value (MV) % R = MV I SV x 100 MV=A-B (acceptable range (m!!CaCO JIL) =75 to 125%) C\3 CJ3.o"Jo Samp l e volume Beg i n End Tota l Alkali n ity . (ml) Samp l e number Samp l e ID ml ml ml Multiplier (meCaCOVLJ tl \5 h\1 A--SQ0 \ 3 OlS:v -:3.1 ( tj'_t:j lQl f\ C:,.tn.

  • 0'1-li"JA.5&u \ ..(.8 $ 1\

"2. '2. \ z. .3.1 tal I \ b '"\t-. l t lo I 3 g,l loi uu \ ltj) .:W.v 40.1-lt .i 0S*U1.* \\ \ 'Z 1 4t:1 lto as.-l\ ) -3 I J.ll 5.& 5! s-r ....--. \\Q'Sb-:z..C)\ v\)\ 5.&' 'B!+ :v.o I?..\ 1\0Soo.{. '2...\ 1 z. I 1 1.'5 3.i l .__ La I R ev i e w e d by: Dat e r e v i ewed: I 05 -oq.l \ Page 68 of 1 00 S OP C 6 -Exhibit C6.1, r e vi s i o n 09-0 1-0 9 I ' I! t l f! I I ") ') *I; ' . . .J Env i ronm enta l Tes ti ng So l utfons. I nc. Analyst ..... __ -; Date analyzed bs*. cg. 1 1 Alkalinity (SM 2320 B) Matrix: Water , RL = 1.0 mg CaC0 3/L Titrate samples to pH=4.5 S.U. Page _4,_q!.....__ Page 4 of_Lj__.__

  • _ Time in i tiated Time completed L-------'

Titrallt normality and multiplier determination: bli.of Normality Normality (N) pH Factor or Multiplier Deionized Titrant checK "'* _Total = (5 ml Na1C0 3 x 0.05)/E = (N x 50000)/100 ml sample DO:!;IJJ -=-water reference standard ml ml ml =0.25/E =Nx 500 4.5S.U. number number (E) (acceptable range 'u.u l<.v--L b L d d a oratory contro stan ar : Reference standard True value Sample Alka lini ty (MV) % RS= MV /TV x lOO number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJIL) (ml) ml ml m l =90 to 110%) J:" N"S.S 100 100 11.5 4.<1 ceo qo.ovfo Alka lini ty %RPD = Multiplier (mg CaCOJIL) {(S-D) /[(S+D)IllJ x 100 (acceptable range=:!: 10%) Mit. 'k a ru: STJl e recovery: Reference standard Spike value Sample Spike alka linit y (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (mg CaCOJIL) (ml) ml ml ml ICO so 314 1, 1!-) Cl.'1 .l; I SO Samp l e alkalinity (B) Measured s pike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOJIL) =75 to L2S%) cu Ct\.0°Io s l ample measurements: Sample volume Begin End Total Alkalinity Sample n umb er Samp l e ID {ml) ml ml ml Multiplier (riig CaCO,VL) \ \ DScn.. en.. rrJA bJi lA.) \ 314 34:o .;?,(.:. 2.) q .C( 1\ '2-L.. \ z I 3Lf.o 31.o 3.0 sq 1\0Sol... I \o \ .3 3-:J.,o J.fi) :2. 3.-z.. (Q5 bmrt. CclAr. Ito .o-kl.'Z-%.c 51-<JPAc l>f.Gl d).O (o .I laO fPDt. R'l.O \Q.o sq \!/ ,/ mt>Ac 6'8 h.% 120 1\ .* 2.4. tl}.) '\ cso"f. I&' {h£J1Ll1aiL d ..L 5.1.. I .... .:-6(10 Re v iewed by: \ Date reviewed: (')5 w 0,. Lt Page 69 of 1 00 SOP C 6-Exhibi t C6. I , revision 09-01-09 I t ! I 1 -I ' *r I E.nvfronmentaiTe.stJng Solutions, In c. Analyst I Date analyzed o5 _ 0-g *11 Total Hardness (SM 2340 C) RL = 1.0 mg CaC0 3 1L Page Page of_4 __ Time initiated I 0 Ol.f* Time completed Titrant normality and multiplier determination: 1 O\lT AtC:.etmr-lte.. LIMIT'S. ASC:.o<.J f\Te. t) .tt AI'J AL 'I t>-e Titrant Nor mality check Begin End Total N ormality (N) of EDT A pH Facto r or Multiplier reference standard m l ml m l = 0.2/E = (N x 50000)/ 50 ml sample number number (E) (acceptable range= 0.0180-0.0220) =Nx 1000 *.rtJ jt .y. .23 ..:r.vss D.l cr.q q;g 2D.lf L h t I d d a ora ory contro stan ar : Reference standard True value Sample Hardness (MV) % RS= MV /TV x 100 number (TV) volume Begin End Total Multiplier (mgCaCOYL) (acceptable range (mg CaCOJIL) (ml) m l ml ml =9 0 to 110%) TIVSS. tp1G) 40 50 c, , q q ., ..... .... ,... -" II .lop '"'1Qs,v ..JI "'lC.."V-10 ' \."1 . 21S "1-&1.S 7 .. Sample Hardness %RPD= Sample Sample ID volume Begin End Total Multiplier (mgCaCOYL) {(S-D) /[(S+D)/21} x 100 ml os.os.tJ Ssw I:'"} 2u.c.J. 35 Duplicate (B) 1.-:f J.. D :35 Mi . 'k atnx sp 1 e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Mtdtiplier (mg CaCOYL) (mgCaCOYL) (ml) m l m l ml r tJ$5 cw1 140 5o 13..3 (1.0 3.-:t-2i).Y. Samp l e h.ardness (B) Measured spike value (MV) % R=MV /SV x 100 (m gCaCOYL) MV=A-B (acceptable range (mgCaCOYL) =75 to 125%) 35 168.

  • (

s, l ample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (me CaCOJIL) TV=ND Blank (should be= 0 mg CaCOJIL) ff) t-:} ,i) 11-.o O.o .Y. f0() bS-oS-1> (n\1$\)J I \-:f.i) o:>J.l. J-t .'2. \A ,;1 .;(5.; Jt.l <64-OS .lf.\ . \\ LJ-.2... '8'(o l)lt '2.1*') $5.1-J-t. 2., b'-{ .l.-(pJ\ 33.1-....... 3 06.02--l \ A 3$'0 4-.?... tt'S.O'Z-1 \ ..(3 . \ 'iN* os.o.3.t\

  • 0.0 4.1 4.1 ()$.02.*1

\ ssw '{.l 5.1 1.1-..;I..-35" is used, sample mu s t be diluted. Reviewed by: Date reviewed I os-o& -II SOP C7-Exhibit C7.1, revision 09-01-09 i i I I iETS ) Environmental Testing SoJut lo ns, Inc. Analyst Date analyzed 0 5 . c 8 . 1 1 Total Hardness (SM 2340 C) RL = l.O mg CaCO/L Page 35 Page 4 Time initiated Time completed T.'t t r 1 ran norma tty an d lt'l' d mu :tpper eterm nation: Titrant Normality coeCK F.nd reference standar d ml ml number number Lab I d d oratory contro stan ar : Reference standard True va l ue Sample number (TV) vo lume (mg CaCO,!L) (ml) :!N$ q 1 'I 40 50 Mi. 'k atrlx sp_t e recovery: Reference standard Spike value Sample numb er (SV) volume (mg CaCOJIL) (ml) :I"NSS<11q Sv Sample hardness (B) (mg CaCO,!L) s l measurements: Total Normality (N) of EDT A pH Factor or Multiplier mJ -0.2/E -(N x 50000)/ SO m l samp l e (E) (acceptable range

  • u. n A')'l0) =Nx 1000 Hardness (MV) % RS= MV /TV x 100 Beg i n End Total M ultiplier (mg CaC03/L) (acceptable range ml ml m l =90 to 110%) 1-.1-J.q 3'1 'i:J ,Sb/o Hard.ness %RPD= Mu l tiplier (mg CaCO,!L) {(S
  • D) /I(S+D)/2)}

x 100 Begin ml \d-.4 End Tota l Multiplier ml ml lq,D 2,1),\f-Measured spike valu e (MV) MV=A-B s D Spike hardn ess (A) (mg CaCOJIL) % R = MV I SV x 100 (accepta ble range =75 to 125%) Sample volume Begin End Total Hardness Sample numbe r Sample ID (ml) ml ml ml Mu l tiplier (me CaCOJIL} Blank . (should be= 0 mg CaC03/L) llCSOS. 0'7-L.l.h..Uw

1. 8) 1<1.0 cQ3.1 ,Lf.'} C}tp 1\ ()$()1. 0'1-l 3 I n l

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  • crz_ I \J Z 3.2..\f atp::r -4.1 l I ':to I\ OS<l). 0\ \ .3 \ J-1-J .I .If!+ I ll (YS (f2.
  • 0 \ rrJ A

\0\ \ g) 5$" l\OSQ4. 21 I z. .!.£$ .Lllu.q 3.\ ll($ \\0'5ot:.. tS J 3 ().0 3.$' 11 oso-z.. o'L ctJt \ 3.'> to.2. ....;- is used, sample must be diluted. Reviewed by: \1(.--A._ Date reviewed I Ql3-Oq.l( SOP C7-Exh ibit C7.l, revision 09-0!-09 I ,. ' ' ' i I I l ' I I I I ' -I I .ETS

  • 9 l ) *,. . } Envlronmtntaltening SoJutfons,lnc.

Analyst I f36L-Date analyzed t-()-"'$=. t>'-8---, -, ----1 Total Hardness (SM 2340 C) RL = l.O mg CaCOiL Page 3"' Page 3 of_'+ __ Time initiated 'b..,_ Time completed ll--=-c::::::::::::::o::::::::::-- -:---i Tt 1trant normality and multiplier determination: Titr,.nt Nnrm<>litv c heck Begin End Total No rmality (N) of EDT A pH Factor or Multiplier r efe rence standard ml 1111 =0.2/E = (N x 50000)/50 ml sample number number (Ef ... le range 0.0180 -0.0220) Nx 1000 .. L b I d d a oratory contro stan ar : Reference standard True value Sample Hardness (MV) % RS = MV I TV x lOO number (TV) volume Begin End Total Multiplier (mgCaCOYL) (acceptable range (mg CaCOYL) (ml) ml ml ml =90 to 110%) .:I'NSS<=hc\ 40 50 \....2. +_q i.-=i-20:4 35 . I. q 3'1 Hardness %RPD= Begin End Tota l Multiplier (mgCaCO)!L) ((S -D) /[(S+D)/2)} X 100 m l ml ml 3.\ s lle*0 3.\ j_ D 1.*3 atrrx sp1 e recovery: Reference sta ndard Spike va lu e Sample Spike hardnes s (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) (mgCaC O)!L) (ml) ml ml ml J'rJS6CjiCJ l-l-0 5o 11..CJ S:o 2\:14 11::>0 Sample hardnes s (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCOJIL) MV=A-8 (acceptable ran ge =75 to 125%) v3

  • 3'1-q2.5°/o S I a1tlp_Je measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (meCaCOYL) Blank" ' TV ND (should be ' u n Jl. I\ bSe>lo. I \.o 11\JA 3 6) 2.) .2., 3.?> 2o.4 tu1-OS*<rZ.*\1 A lf'O l-\S\j) l)\) ) 2.1.2. .,Q5.'] "+.1-9\p OS*cn*ll & \ z .:>7551 &>.3 14.'+ Clo \ 3 Bo.3 3-+1-J..f.t..t qo \\DScn.o \ IOl\)V l 34.1-3.\ I) "2..l \ "l--3r-R 41-1 14 "OSo'-. \S \ 3 .3 .-:} \ 3-1 -t Jl)';,. ()2. 0'2.-D.Cl :3. i "2.2 \ z. 3.\ L-'5 3.4 -loct is used, sample must be diluted. Reviewed by: ..._I Date reviewed I SOP C7-Exhibit C7.1, revision 09-01-09 } l ' i I ! i ; l ' ' i I I -I I !ETS Environmenta l Testing So h.rUon.s. fnc. Analyst I f£4.. Date analyzed r-()=5-.c:g--. -1\---; Total Hardness (SM 2340 C) RL = 1.0 mg CaCO i L Page 31-Page 4 __ Time initiated I Time completed itrant normality and multiplier determination: u *. ""* **"'check End Total Normality (N) of EDT A pH Factor or Multiplier reference standard ml m1 "" 0.2/E (N x 50000)/50 m l sample number number (E) (acceptable range "* 1111?')0) Nx 1000 -L b I {/, d a oratory contro stan ar : Reference standard True value Sample Hardness (MV) % RS=MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaCOJIL) (ml) m l ml ml = 90 to 110%) .'IIVS:Sqi1 40 50 IR6 Ra.t l.q 2-D !-/ 3q cn-sr;,lo Hardness %RPD= End Total Multiplier (mg CaCOJIL) . {(S-D) /((S+D)/21} ll 100 Total Multiplier Samp l e hard.ness (B) Measured spike value (MV) % R = MV I SV x 100 (mg CaCDJ/L) MV=A*B (acceptable range _(mg CaC01/L) = 75 to 125%) cro J.i'tl \o0 ,1) s l amp1e measurements: Sample volume Begin End Total Hardness Sample number SampleiD (ml) ml ml ml Mu l tip l ier Cm2 CaCDJ/L) TV -:-li.Tr\ Blank i SDOUIU n 1\ Ob H. . 6'-\ Drn a.. dd AC. *b /f5 .i '+.'5 k_\). tJ qz. I \ {>pf\C I Jl";f.C i qo .L I {)Pet-I d'i-.0 J.f."" J-CJ4-v---'--'{350 is used, sample must be diluted. Reviewed by: ._I _Ba.=b'--::.._ ___ ....J Date reviewed I Q5.Qq.L \ SOP C7-Exhibit C7 .l, revision 09-01-09 1 1 . 3 . ' J l :': I I , , ll* f *Ers * * > ' J f'* *. etlvfronmtnt111Ttitlng Solutkms, fnc:. Analyst 11/i£ Total Hardness (SM 2340 C) RL = 1.0 mg CaC03/L Time initiated I oqa5

  • Date analyzed 1 Titrant normality and multiplier determination:

Titrant Normality check Begin End Total reference standard ml number number c 1'-1 e. 0.0 Lb I d d a oratory contro stan ar : Reference standard True value number (TV) (mg CaCOJIL) IN') SCilti 40 Mi . 'k atrTX sp1 e recovery: Reference standard Spike value number (SV) (mgCaCOJIL) l 40 ml Sample volume (ml) 50 Sample volume Sample volume (ml) R) ml (E) qq Begin End ml ml 1\.9 Begin End ml ml Time completed Normality (N) of EDT A pH Factor or Multiplier =0.2/E = (N x 50000)/ 50 ml sample (acceptable tange = 0.0180-0.0220) =Nx 1000 Q.ozo2. Hardness (MV) % RS-MV /TV x 100 Total Multiplier (mg CaCOJIL) (acceptable range ml =90 to 110%) A-0 &.{0 IOO.o'/. Hardness %RPD= Multiplier (mg CaCOJIL) {(S -D) /[(S+D)/2]} X 100 20.2, s D Spike hardness (A) Total Multiplier (mgCaCOJIL) ml Lt*O -zo.z. et Sample hardness (B) Measured spike value (MV) % R = MV I SV x 100 (mgCaCOJIL) MV=A*B (acceptable range (mg CaCOJIL) 75 to 125%) "iZ.. :3q £11-.S/: s. l amp, e measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (meCaCOJIL) TV=ND Blank 9.) o.o (should be= 0 mg CaCO J IL) O.o o.u r"'-J 05.QS.\l 1'-lli 18.0 2.'1..'5 &.ts C)\ OS. A a-s ?.':\ .t &.\.v ll'S .\f:\. II 0 ln.l *"' "l.') ql 04 *2.1.\\ 3l.\l '-t.q t¥l 04*fu .\\ qful 4.4 0'5 .ctl.*l fi -ao. l C11 18 ;o.\ 3'1.5 q,cj BCi v 3-t.S 3q,z_ Gt$ Ssu Z..t ,I; i.IZ.. 551f <>feJG11t is used, sample must be diluted. Reviewed by: L----r'::lt-'\ __ -1 Date reviewed I 'OS.* c'\* 11 D'\ SOP C7-Exhibit C7.1, revision 09-01-09 ..: Page 75 of 100 Sequoyah Nuclear Plant Biomonitoring May 03-10,2011 AppendixD Reference Toxicant Test and Control Chart

  • * * ) Pimephales promelas Environmental Testing Solutions, Inc. Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc. -u 'bo -11'1 N u > "' "C I ....
  • 0.9 0.8 0.7 0.6 0.5 1.4 1.2 1.0 0.8 0.6 0.4 1.4 1.2 1.0 0.8 0.6 0.4 USEPA Control Limits(+/- 2 Standard Dev i ations) ..... ............................

................ . . . . .............. ................... ............ ' ..................... ................. . ....... USEPA Warning and Control Lim.its (75th and 90th Percentile CVs) ... ---.... ............... ...................... ............... .............. ....................... ..... . **.-._.,_ ... _.,_.,_,,_,,_, ,_,,_ .. _ .. -**-**-**-**-**-**-**-**-**-**-**-* -**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-* ........................................................................................... ....... Laboratory Warning and Control Limits (lOth and 25th Percentile CVs) ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . -**---**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-* .......................... .................................. .......................... ............ Test date 7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC 25} Warning Limits (mean IC25 +/- sA.lO or sA.75) Control Limits (mean IC 25 +/- SA.25 , SA.90 , or 2 Standard Deviations} aenel'ited from assoc i a t e d ex cel spreadsheet. Exce l spreadshe e t en te red b y: J. Sumn*r Review e d by: --¥---

  • * * @11 G ETS Environmental Testing Solutions, Inc. Test number Test date 7-daylC,. (g/LKCI) cr (g/L KCI) 1 2 3 4 5 6 7 8 9 10 11 u 13 14 15 16 17 18 19 20 09-14-10 09-21-10 09-28-10 09-29-10 lQ-05-10 1o-o6-10 1Q-12-10 lQ-26-10 11-()2-10 1Hl9-10 12-<17-10 01-18-11 01-25-11 02-QS-11 02-15-11 03-os-11 03-os-11 03-29-11 03-31-11 05-03-11 0.75 0.73 0.65 0.68 0.70 0.72 0.70 0.68 0.70 0.75 o.n 0.78 0.70 0.69 0.68 0.71 0.70 0.75 0.75 0.78 0.74 0.71 0.70 0.70 0.70 0.70 0.70 0.70 0.71 0.71 0.72 0.72 0.71 0.71 0.71 0.71 0.71 0.71 0.72 5 0.02 0.05 0.05 0.04 0.04 0.03 0.03 0.03 0.03 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 0.04 I, Plmephales promelas Chronic Reference Toxicant Control Chart State and U5EPA Control Limits CT-25 0.71 0.60 0.61 0.62 0.63 0.64 0.64 0.64 0.64 0.64 0.64 0.64 0.64 0.64 0.64 0.64 0.64 0.64 0.64 CT+25 0.71 0.82 0.79 0.78 0.78 o.n 0.76 0.76 o.n 0.78 0.80 0.79 0.79 0.79 0.79 0.78 0.78 0.79 0.80 0.09 o.os 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 laboratory Warning Limits cr-5.uo cr + 5.uo 0.65 0.62 0.62 0.62 0.62 0.62 0.62 0.62 0.62 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.83 0.79 0.78 0.79 0.79 0.79 0.78 0.78 0.79 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.80 0.16 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 laboratory Control Limits cr-5..., cr+5..., 0.58 0.56 0.55 0.55 0.56 0.56 0.55 0.55 0.56 0.56 0.57 0.57 0.56 0.56 0.56 0.56 0.56 0.56 0.57 0.89 0.86 0.85 0.85 0.85 0.85 0.85 0.85 0.85 0.86 0.87 0.87 0.86 0.86 0.86 0.86 0.86 0.87 0.87 5.._75 0.28 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 0.27 U5EPA Warning Umlts cr-5.._75 0.46 0.44 0.43 0.43 0.44 0.44 0.43 0.43 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.45 CT+5A.75 L02 0.98 0.91 0.97 0.97 0.97 0.97 0.91 0.97 0.98 0.99 0.99 0.99 0.98 0.98 0.98 0.98 0.99 0.99 Note: 7-d IC,. 7-day 25% inhibition concentration.

An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. CT Central tendency (mean IC 25). 5 Standard deviation of the IC 25 values. laboratory Control and Warning UmJts 5.._.. 0.33 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 0.32 U5EPA Control Umlts cr-5.._.. CT+5.uo 0.41 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.40 L07 1.03 1.02 1.02 1.02 1.02 1.02 1.02 1.02 1.03 1.04 1.04 1.04 1.03 1.03 1.03 1.03 1.04 1.04 cv 0.02 0.08 0.07 0.06 0.05 0.05 0.05 0.04 0.05 0.05 0.06 0.05 0.05 0.05 0.05 0.05 o.os 0.05 0.05 Laboratory control and warning limits were established using the standard deviation of the IC 25 values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. S.uo Standard deviation corresponding to the 10"' percentile CV. (s._., 0.12) 5...,. Standard deviation corresponding to the 25"' percentile CV. (S..,. 0.211 U5EPA Control and Warning Limits SA.75 Standard deviation corresponding to the 75"' percentile CV. ( S._15 0.38) Standard deviation corresponding to the 90"' percentile CV. (S....., = 0.45) CV Coefficient of variation of the IC,. values. USEPA. 2000. Understanding and Accounting for Method Variability tn Whole Effluent Toxldty Applkatlons Under the National Pollutant Discharge Elimination Prosram. US Environmental Protecllon Ondnnatl. OH. Page 77 of 100 obtained from Aquotox.Inc. rile: l!llleredby:J.s'T R<vicwcdby: ' 1 --r: 0 ... "' 'i:: "' > -0 ... r: Gl 'u E Gl 0 u * * * * .r: i 0 ... (,!) 0 ... ... r: 8 ... .E Pimephales promelas Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Organism Source: Aquatox, Inc. 1.00 . ............... .......................... ..... . **************************************

    • 0.75 ................

...... . . . . ................................... . 0.5 0 0.25 USEPA Acceptance Criteria (> 0.25 mg per surviving larvae) 30 Kentucky Acceptance limit(< 30.0%) 20 10 . ............................ .......................... ' ................... ........... ....... . ** Ill **************** 0 30 USEPA Upper PMSD Bound (90th percen t ile < 30.0%) -20 c .............. .............................. ............. ....... . II) a.

  • 10 ***************************************
                              • t ************************************** Control Reproduction, Coefficient of Variation (CV}, or Percent Minimum Significant Difference (PMSD} PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

Central Tendency (mean Control Growth, CV, or PMSD) ......... Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) Page 78 o 100 Gra phs 1 ene rat ed from as.soclated spreadsheet. Exee l spreadshee t enKred by: J. Sumner Revlowe<f b y: Q) iETS Precision of Endpoint Measurements Pimephales promelas Chronic Reference Toxicant Data ) Envlmnmental Testing Solutions, Inc. Test number Test date Control Control Mean cr cv cr Survival Growth (%) (mg/larvae) for Control Growth (%) for Control (mg/larvae) Growth CV (%) 1 09-14-10 97.5 0.813 5.9 2 09-21-10 100 0.891 0.852 7.5 6.7 3 09-28-10 97.5 0.991 0.898 9.5 7.7 4 09-29-10 100 0.893 0.897 8.5 7.9 5 10-05-10 100 0.912 0.900 8.1 7.9 6 10.06-10 100 0.810 0.885 2.3 7.0 7 10-12-10 100 0.799 0.873 4.1 6.6 8 10-26-10 100 0.779 0.861 10.0 7.0 9 11-02-10 100 0.727 0.846 10.9 7.4 10 11-09-10 100 0.761 0.837 6.3 7.3 11 12-07-10 100 1.080 0.860 8.3 7.4 12 01-18-11 97.5 0.806 0.855 5.8 7.3 13 01-25-11 100 0.608 0.836 4.6 7.1 14 02-08-11 100 0.817 0.835 2.6 6.7 15 02*15-11 100 0.701 0.826 3.3 6.5 16 03-08-11 100 0.953 0.834 3.1 6.3 17 03-08-11 100 1.006 0.844 4.7 6.2 18 03-29-11 100 0.829 0.843 6.3 6.2 19 03-31-11 100 0.675 0.834 3.2 6.0 20 05-03-11 100 0.867 0.836 6.3 6.1 Note: 01 Coefficient of variation for control growth. Lower 01 bound determined by USEPA (lOth 3.5%. Upper 01 bound determined by USEPA (90th percentile) 20% MSD Minimum Significant Difference PMSD Percent Minimum Significant Difference MSD PMSD cr (%) forPMSD(%) 0.11 13.9 0.07 7.5 10.7 0.11 10.9 10.8 0.11 12.1 11.1 0.09 9.5 10.8 0.06 7.4 10.2 0.10 12.0 10.5 0.13 16.8 11.3 0.11 14.5 11.6 0.12 15.4 12.0 0.15 *13.9 12.2 0.09 11.0 12.1 0.08 13.2 12.2 0.06 7.8 11.8 0.12 16.8 12.2 0.08 8.4 11.9 0.13 12.7 12.0 0.09 10.3 11.9 0.09 12.8 11.9 0.08 8.8 11.8 PMSD Is a measure of test precision. The PMSD Is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10m percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile) = 30%. CT Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-Q0-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-8-01-QOS. US Environmental Protection Agency, Cincinnati, OH. File: ppkclcr_OS0311.xlsx Entered by: 1. Sumner Reviewed by:---+- I I i I I I 1 I I I I -I I I I I I I I I I Solvt l onr. Inc. Page I of5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas PpKClCR Test Numb e r: Dilution pr e paration information: Comments: KCl Stock INSS number: INSS. '\'2.Q-S tock preparation: SOg KCIIL: Dissolve 50 g KCI in 1-L Milli-Q water. Di lut ion prep (mg!L) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluen t volume (mL) 991 988 985 982 979 Total vo lu me (mL) 1000 1000 1000 1000 1000 Test organism information: Test iliformation: Organism age: \\O\l4t.C:. 0\.b Randomizing template: R.e..b Date and times organ i sms were* born between: OS*CS'l.*\\ \\oQO Incubator number and .3F shelf location: Organism source: .-.._"Tl)y. OS-<n.*\\ Artemia CHM number: Drying information for weight d e termination

Transfer vessel pH= '"'l.t. s.u. Date I Time in oven: 0'*10*\\

information: Temperature = "1... oc Initia l oven temperature: \oO'( Average transfer v olume: o. ,,, ... Date I Time out of oven: l**hs Final oven temperature: \oO 'C. Total drying time: Daily feeding and renewal informat i on: Day Date Morning feeding Afte rnoon feedi.ng MHSW 0 2 3 4 5 6 7 Pag e 80 of 1 00 SOP A T21 -Exhibit A T21.1, revision 04-01-09 ii-I ' I ' *':. '!fr' ' I I I I I -I I .ETS * . ' 3 . J *. . ".** ,..___.;-7r'

      • .... * . . . . EnvlllmmontollUting 5ollllian$.1n<.

Page 2 of5 Species: Pimephales promelas PpKCICR Test Number: Survival and Growth Data Day Corttrol 450 KCIIL KCIIL A B c D E F G H I J K L 0 /0 I() /() 10 /() 10 l() It) ID 10 I() 10 1 to fO to 10 (0 10 10 lb tO lD tO ro 2 /0 10 10 to to /() 10 10 IO IO 10 *ro 3 /0 10 10 I() 10 I() I() 10 tO /Q 10 10 4 IQ 10 10 /Q IQ 10 10 /Q IC /0 10 ID 5 /() 10 (() to 10 gltl.. . 'ct. lO IC l() 6 ID 10 IC to lO ,.II( /Q ID &'1. 'l '\ 7 /D IC ll..fr to to '"' g 4 '\ 'i '"' (() /C lC A= Pan weight (mg) Tray color code:: Pl-1&11.1. Analyst: 13*t1 l4.0£t 14-.c;; t3.'H t;.1-l t+.4r \4-At, Date: fl S! 9:! -'l(ij B = Pan + Larvae weight (mg) Analyst: cDI u.1't tS*II1 *v..s* it !It 2t.tM 22.6'1-121.2'f izt.OS 2l.SO n..'fe6 Date: C = Larvae weight (mg) = B-A Hand calculated. 8.1.'\ t;.'\1 .tAl. ,,,\. a.oC\

t. 14'{ c-.'1'\ c.. ,,Sl t.oto Analyst: ejl...._ Weight per initial number of larvae (mg) = C /Initial number of larvae

""'1, "'" ",, ,_e.., Hand -* /'" " \: q; '-0 " \: Analyst: 0' o* ()' (). ()* a o* o* \)' o* 0' o* lJ' Average weight per Initial Percent number oflarvae (mg) reduction o.1t'2. 'i.& 1. l1. t 7. from control (%) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: Page 81 of 100 SOP A T21 -Exhibit A T21.1, revision 04-01-09 I I i / l I l j 'l 1 I ; ' ., ! :tf. -I I IF ' I I I I I -I I )!£; . Page 3 of5 } E"nvl.,n....,taJTulfngSollllfono.ln<. Species: Pimeohales oromelas PpKClCR Test Number: 1.'!. \ Survival and Growth Data Day 750 mE KCIIL 900ml! KCIIL 1050 m KCIIL M N 0 p Q R s T u v w X 0 It> lb /0 IO I() /0 10 lb /0 10 10 IO 1 /0 IC It> , tf\ I() /0 ,,tA 10 arA *k\ i 2 ID 'I i (a IO 10 /0 10 lo 3 4\'"' 'i'G(, tl\.A '\ JO 4 10 "\ '\ '\ '" "\.-.. , 5 5 ID Dt q '\ ,lt.\ t\ s'f\ g. 3 6 '\ bt.l \i{ '4't.( '\ '\ (.. i 3 7 q'" t\ ql$1'1 "" t""' .gf#t IV\ *2P\ (p (, '-A-Pan weight (mg) t'wM Tray color code:: Analyst: 13.01 1+.4& 14A4 13 * .;t.. l4-A*S 13.'ft l4At t';.fl t5A? Date: B -Pan + Larvae weight (mg) Analyst: (!,OJ"' 200-l 20,\gf' 2.2.-IS' lr.'tO lt*'51 lll.l1-IS"J5Ct u, .lt+ 111-:1-tP Date: n5 C = Larvae weight (mg) -B-A Hand calculated.AI S,llo 1.wo (1. "2.'( \.iS ,.aa \.U.. t.c..t Analyst: "' _I Weight per Initial number of larvae (mg) = C I Initial number of larvae 'fa\ '-\ /::-., .l_t> v,_, ,..,, Hand calculated/\) 1 c; \D i'\ '\ \ \: Analyst: ,_. ()" o* ()' t;)' o* ()* ()* o* I)' o* o* A Average weight per initial Percent number oflarvae (mg) reduction O.bifo 'Z.O ,'\ 7. . C), & \. '2.7. from control (%) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded; Comments: Page 82 of 1 00 SOP A T21-Exhibit AT2l.l, revision 04-01-09

  • *
  • 8 u Enviro nm ental T esti n g So l u ti o ns, I n c. ---IICI) CGntrcl 450 600 750 900 11150 Dun.-'sMSD

,.._ I'MSD: -A 8 c D E F G H I J IC L M N 0 p Q R s T u v w X ............ -10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 0.0764 a.a Page 83 of 100 ..... _._ .. -10 10 10 10 10 9 10 10 8 8 9 9 9 9 9 9 6 6 6 8 3 3 2 2 ...................... 13.n 14-2S 14.20 14.119 1453 13.74 13.71 14.10 14.95 14.41 B.99 14.42 1 4.85 13.07 14A2 14.44 B56 14.48 13.92 13.84 14.42 13.71 15.45 UJJS MSO= PMSO= I . P i m ephales pr ame las Chr o nic Refete nce To xicant Test E P A-821-R-0 2-0B, Meth o d 1000.0 Qual i ty Control Verificati on o f Da ta E ntry , Cal c ula tion s, a nd Statistical Ana l yses I * ,_, * &.ara. ..__...,. __ ,_ --A*l 2230 853 0.853 &.24 QB24 23.!;7 9A1 0.947 22.51 8A2 0.842 21.1i9 7-16 0.716 21.83 8.0!1 0.899 2130 7.59 0.759 22.54 L44 0.844 21.24 &.29 0.786 21.0S 6.64 0.130 21.50 751 o..B34 22A8 8.06 0..8:96 20.71 5.86 0.651 20.ru 7.60 0..844 22-lS 7.73 0..859 20.68 6.24 0.693 17.90 4.34 0.723 1851 4-.03 0.672 18.85 4.93 D.B22 21.28 7.44 0..930 16-17 1-75 0583 15.59 L88 0.627 16.!;7 L22 0.610 14.76 1.68 0.840 Minimum Silnific:ont lliff-..ce M" ........ Siplificant Diffennc:e ---.......... _._ _, ..... -. .. ............... .............. _......... _...., _"'...._."" 0.&53 OJI67 6.3 0.824 0.947 0.842 0.716 0.804 10.2 0.809 0.75!1 0.844 0.629 0.137 5.4 Q.664 0.751 0.1106 0586 0.762 13.8 0.760 o.n3 0.624 OA34 0.787 145 0.403 D.493 0.744 D.17S 0.665 17.7 0.188 0.122 0.168 Test rwml>or: _______ Test _: _______ ---1>< ---.._. .. _._ _......__ _.,_ ClQ -101 -100.0 OM7 6.3 Net appllatH 97.5 0.78Z 7.l 9.1 85.0 0.713 ll.3 17.1 9Q.O 0.6&6 13.1 20.9 6SJ) 051.9 29.9 40.2 5.0 0.163 17.6 ll.l PMSO is* II>NSUOW oltat pt<Cision. The PMSO is the mbmum _, clilltAnc. between tho oonuclond _,tNt can bededortd stotlslbly ..,..-_,,in

  • whole __ , tOIIicity

...._ .__.,.PMSObouodde-byUSEPA(10dl-tSo) =12%. Upper PMSDbou>d deCermined 3""-lDw .. File:: pptcb_OS0311.xlu Eauad by: J. s. ...... Rovie>>'Od iETS ,) Elwlronmonlal 'IMtlng Sollltlons,tnc. Start Date:. 51312011 Test ID: End Date: 5/1012011 Lab ID: Sample Date: Protocol: Comments: Cone-I 2 3 D-Control 1.0000 1.0000 1.0000 450 1.0000 0.9000 1.0000 600 0.8000 0.8000 0.9000 150 0.9000 0.9000 0.9000 900 0.6000 0,6000 0,6000 1050 0.3000 0.3000 0.2000 Mean N*Mean Mean D-Control 1.0000 1.0000 1.4120 450 0.9150 0.9750 1.3713 0 600 0.8500 0.8500 1.1781 0 750 0.9000 0.9000 1.2490 0 900 0.6500 0.6500 0.9413 0 1050 0.2500 0.2500 0.5216 Auxilimy Tests Shapiro-Wilk's Test indicates nonnal distribution (p > 0.0 I) of variance cannot be confirmed HlJ!othesis Teat O.OSl Steel's Many-Qne Rank Test Treatments vs D-Control Trim Level EC50 0.0% 5.0% 10.0% 20.0% Auco-15.0% 949.51 NOEC 450 95%CL 891.54 l0ll.l6l Statistical Analyses Larval Fish Growth and Swvival Teat*7 Sunival PpKC!CR SampleiD: ETS*Envir. Testing Sol. Sample Type: FWCHR-EPA-821-R-02-013 Test Species: 4 1.0000 1.0000 0.9000 0.9000 0.8000 0.2000 Transfonn: Arcsin Sguare Root Rank Min Max CV"A> N Sum 1.4120 1.4120 0.000 4 1.2490 1.4120 5.942 4 16.00 1.1071 1.2490 6.954 4 10.00 1.2490 1.2490 0.000 4 10.00 0.8861 1.1071 11.742 4 10.00 0.4636 0.5196 12.838 4 10.00 Statistic 0.94239873 LOEC ChV TU 600 519.615242 Trimmed Spearman*Ksrber REF*RefToxicant KCL-Potessium chloride PP*Pimephalea promelas !-Tailed Critical 10.00 10.00 10.00 10.00 10.00 Critical 0.884 Number Total Re!J! Number 0 40 40 6 40 4 40 14 40 30 40 Skew Kurt 0.46929573 0.8338!121 File: ppkclcr_ 0503ll.xlsx Entered by: J. Sumner Reviewed

  • * *
  • TS Envl,..nmeniOilUdngSofutlono.lnc.

Start Date: 51312011 End Date: 5/1012011 Sample Date: Comments: Cono-D-Contto! 0.8530 450 0.7160 600 0.6290 1SO O.S860 900 0.4340 IOSO 0.!7SO 2 0.8240 0.8090 0.6640 0.7600 0.4030 0.1880 ConC=!!!I!IL Mean N-Mean D-Control 0.866S 1.0000 *4SO 0.7820 0.9025 600 0.7125 0.8223 7SO 0.68S8 0.7914 900 O.S18S O.S984 IOSO 0.!633 0.1884 Auxiliary Tests Tesi!D: Lab!D: Protocol: 0.9470 0.7S90 0.1SIO 0.7730 0.4930 0.1220 Mean 0.866S 0.7820 0.7125 0.68S8 0.5185 0.1633 Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01) !'-Test indicates equal variances (p = 0.97) Hypothesis Test (1-lail. O.OS) Homoscedastic I Test indicates significant differences Treatments vs D-Conttol Statistical Analyses Larvall'ish Orowth and Surviwl Test-7 Day Growth PpKCICR Sample ID: ETS-Envir. Testing Sol. Sample Type: FWCHR-BPA-821-R-02-013 Test Species: 4 0.8420 0.8440 0.8060 0.6240 0.7440 0.1680 Transform: Untransfonned Min Max CV% N 0.8240 0.9470 6.34S 4 0.7160 0.8440 7.180 4 0.6290 0.8060 11.330 4 O.S860 0,4030 0.1220 0.7730 0.7440 0.1880 13.806 29.874 17.593 4 4 4 Slatistic 0.929S9601 1.04289341 MSDu 0.0763S283 Linear Interpolation (200 Resamples) t-Stat 2.1Sl MSDp 0.088!1637 Point ms/L SD 9S%CL(Bxp) Skew !COS* 230.72 96.49 103.78 614.32 0.9S07 ICIO 4S4.64 81.89 211.64 691.81 -0.036S !CIS S48.1S 86.S8 396.44 894.38 0.1S30 IC20 708.22 87.46 443.8S 8S4.07 -0.1925 l*os 782.17 SS.lS 523.40 935.70 -0.7858! IC40 898.74 32.73 790.71 961.7S -0.2823 ICSO 936.00 22.79 8S8.25 984.19 -0.3662

  • indicates

!C estimate less than the lowest concentration RBI'-Ref Toxicant KCL-Potassium chloride PP-Pimephales promelas !-Tailed Criticel MSD 1.943 0.0764 Criticel 0.749 47.46834S64 MSB MSB 0.014280S 0.00308783 Isotonic Mean N-Mean 0.866S 1.0000 0.7820 0.9025 0.712S 0.8223 0.68S8 0.7914 O.S18S O.S984 0.1633 0.!884 Skew Kurt O.S40073SSJ -0.9741049S F-Prob df 0.07SOSI717 1,6 File: ppkclcr _ 050311.xlsx Entered by: J. Sumner Reviewed I I I I I I I I I I I I I I I I I I I .ETS * .) ' jo * ):\' .. -t P,***)* :":* .) Environme ntal Testing Schnlons.lnc. Species: Pimephales promelas CONTROL 450 mg KCVL 600 mg KCI/L 750 mg KCI/L 900 mg KCI/L 1050 mg KCI/L STOCK Page 86 of 1 00 Page 4 of5 PpKCICR Test Nwnber: \ SOP AT21 -Exhibit A T2l.l, revision 04-01-09

. I , ' . ' ' i t--I I .. )!S. ..)

T tilinf Sofutlons. tnc. Page5of5 Species: Pimephales promelas P p K CI CR Test Number: CONTROL 750 mg KCIIL 900 mg KCI/L 1050 mg KCI/L Page 87 of 1 00 SOP A T21 -Exhibit A T2l.l, revision 04-01-09 .; * * * ,. --u c: z --II) N u )loooC c: "0 I t--Ceriodaphnia dubia Chronic Reference Toxicant. Control Chart E nvi r onmental Te sting Solutions, Inc. 1.14 1.12 1.10 1.08 1.06 1.04 1.02 2.5 2.0 1.5 1.0 0.5 1.4 1.3 1.2 1.1 1.0 0.9 .. . . . USEPA Control Limit s(+/- 2 Standard Deviations) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .... . *******************

.** USEPA Warning and Control Limits (75 1 h and 90 1 h Percentile CVs) ************************

.... . .... . . . . . . . . ... . . . . ....... . . . .............*........ ...........*...*......... ..........................***........*

  • .... Laboratory Warning and Control Limits (1 dh and 2 5 1 h Percen tile CVs) *****************************

-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-**-*-**-**-** -**-**-**-**-**-**-**-**-**-**-* -**-**-**-**-**-**-**-**-**-**-**-**-** *************************************************

        • Test date
  • 7-day IC 25 = 25% inhibition concentration.

An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. --. Central Tendency (mean IC 25) -* -.. -Warning Limits (mean I C 25 +/- SA.10 or SA.75) *** *****

  • Control Limits (mean IC 25 +/- S A.25 , S A.90 , or 2 Standard Deviations)

Page 88 of 1 00 Graphs eener.ued from assodattd exc e l spreadsheet. Excel spreadsheet Ied by: J. Sumner Reviewed bv: Environmental Testing Solutions , lnc. Test number Test date 7-day IC u CT (giL NaCI) (giL NaCI) I 2 3 4 s 6 7 8 9 10 II 12 13 14 IS 16 17 18 19 20 12.08-09 01.05-1 0 02..()2-1 0 03.o2-1 0 04.()6.1 0 05-04-1 0 06-0&-1 0 07-13-1 0 OS..OJ-10 09-14-1 0 10.05-10 10.05-10 ll.o2-10 12-07-10 01-1&.11 02.0&.11 03.()&.11 04-05-11 04-05-11 05-03-1 1 LOS 1.08 1.07 1.08 LOS 1.09 1.07 1.07 1.06 1.07 1.08 1.08 1.09 1.06 1.06 1.06 1.05 1.06 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 s 0.02 0.02 U.oJ O.oJ 0.02 0.02 0.01 0.01 O.oJ 0.01 O.oJ 0.01 0.01 0.01 0.01 0.01 O.oJ 0.01 0.01 Ceriodaphnia dubia Chronic Reference Toxicant Contro l Cbart Stateud USE PA uboratory uboratory C ontrol Limits S.uo Warning Limits S.u:s Control Limits S.._75 cr-zs cr + zs cr-s.._,. cr + s.._,. 1.02 1.04 1.04 1.04 1.04 1.04 1.04 1.04 1.04 1.05 1.05 1.05 I. OS 1.05 1.05 1.04 1.04 1.04 LOS 1.11 LIO LIO LIO Lll LIO LIO LIO 1.10 1.10 LIO LIO 1.10 1.10 1.10 LIO 1.10 1.09 1.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.09 0.98 0.98 0.99 0.98 0.99 0.99 0.99 0.98 0.98 0.99 0.99 0.99 0.99 0.99 0.99 0.98 0.98 0.98 0.98 LIS Ll6 1.16 LIS 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 Ll6 Ll6 1.16 1.16 Ll6 0.1 8 0.18 0.18 0.18 0.18 0.18 0.18 0.1 8 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 CT -Sus cr + SA.U 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 1.25 1.25 125 125 126 125 125 12S 125 125 125 126 125 125 125 125 1.25 1 25 1.25 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 UStlPA Warning Umits S 00 CT-Sus CT + S.._n 0.59 O.S9 059 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 O.S9 0.59 0.59 0.59 0.59 l.SS 1.55 l.SS 1.55 1.56 1.55 1.55 I.SS I.SS 1.55 I.SS 1.56 1.56 1.55 1.55 1.55 1.55 1.55 1.55 0.66 0.66 0.66 0.66 0.67 0.66 0.66 0.66 0.66 0.66 0.66 0.67 0.66 0.66 0.66 0.66 0.66 0.66 0.66 N(}{e: 1.0 I Cu 7-day 25% inhib it ion concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Cenoduphniu reproduction for the test population. CT Central tendency (mean IC 25). S-Standard deviation of values. uboratory Co ntrol and Warning Limits US.EPA Control Limits CV CT-S....,. CT+S....,. 0.4 1 0.4 1 0.41 0.41 0.41 0.41 0.41 0.41 0.4 1 0.41 0.41 0.41 0.41 0.4 1 0.41 0.41 0.41 0.41 0.41 1.73 1.73 1.74 1.73 1.74 1.74 1.74 1.73 1.73 1.73 1.74 1.74 1.74 1.74 1.74 1.73 1.73 1.73 1.73 0.02 0.02 0.01 0.01 0.02 0.0 1 0.0 1 0.01 0.0 1 0.01 0.0 1 0.01 0.01 0.01 0.01 0.01 0.01 0.0 1 O.DI l..aborruol)' control and warning limits wen: eslab l isbed using the standard deviation of the values corresponding to the I Oth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the teSt method and endpoinl s.._,.-Standard deviation corresponding to the 10"' percentile CV. (S.._1 0 = 0.08) S.us = Standard deviation correspo n ding to the 25"' percen tile CV. (SA25-0.17) USEPA Control and Warning Limits S.._7 s Standard deviation corresponding to the 75"' percentile CV. (S.._, 5 = 0.45) S.._..= Standard d eviation corresponding to the 90"' pe r centile CV. (S.._90-0.62) CV = Coefficient of variation of the values. US EPA. 2000. Underslllnding and Accounting for Method Variability in Wbole Effluent Toxicity Applications Under the Natiooal Pollutant Discharge Elimination l'rogJam. EPA-833-R..OO..OOJ. US Environmental Protection A&ency. Cincinnati , OH. Pag e 89 of 1 00 File:: CdNit.. ... "'l_OiOJ IJ..ds.x "*"ily-J.-......... ..: iETS _) Environmental Testing Solutions, Inc. Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements -= 0 = = :! = *c e tl = -... = = *-= IB u .. = c! u ......... 40 40 30 20 10 50 40 30 20 10 .. .. ***** ****************************************

... Minimum Acceptance Criteria(> 15.0 offspring per surviving female) North Carolina Acceptance Limit(< 40.0%) Kentucky Acceptance Limit(< 30.0%) USEPA Upper PMSD Bound (90th percentile< 47.0%) *********************************** Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD) Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations) Page 90 of 1 00 Graphs generated from associated excel spreadsheet. Excel by: J. Sumner Reviewed by:

  • Precision of Endpoint Measurements
  • Ceriodaphnia dubia Chronic Reference Toxicant Data Environmental Testing Solutions, Inc. Test Test date Control C ontrol Mea n CT cv CT MSD PMSD CT number S u rv i va l Reproduction for Control Mean for Control (%) (offspring/female)

Reproduction (%) Reproduction (%) for PMSD (%) (offspring/female) CV(%) 1 12-08-09 100 35.2 4.6 2.0 5.8 2 01-05-10 100 31.1 33.2 5.8 5.2 2.3 7.3 6.6 3 02-0 2-10 100 31.9 32.7 3.5 4.6 2.6 8.3 7.1 4 03-02-10 100 31.0 32.3 6.3 5.0 2.4 7.7 7.3 5 04-06-10 100 32.7 32.4 6.3 5.3 2.4 7.4 7.3 6 05-04-10 100 31.0 32.2 4.6 5.2 2.3 7.4 7.3 7 06-0 8-10 100 33.1 32.3 6.6 5.4 2.4 7.2 7.3 8 07-13-10 100 31.6 32.2 4.8 5.3 2.3 7.2 7.3 9 08-03-10 100 28.2 31.8 4.7 5.2 1.8 6.2 7.2 10 09-14-10 100 32.9 31.9 8.7 5.6 3.0 9.2 7.4 11 10-05-10 100 30.2 31.7 7.6 5.7 2.0 6.6 7.3 12 10-0 5-10 100 30.8 31.6 4.0 5.6 2.2 7.2 7.3 13 11-0 2-10 100 30.5 31.6 4.9 5.6 2.0 6.6 7.2 14 12-07-10 100 32.2 31.6 4.8 5.5 2.0 6.2 7.2 15 01-18-11 100 31.9 31.6 6.3 5.6 2.3 7.1 7.2 16 02-08-11 100 30.7 31.6 6.9 5.6 1.8 5.7 7.1 17 03-08-11 100 32.6 31.6 4.8 5.6 2.0 6.0 7.0 18 04-05-11 100 32.8 31.7 5.9 5.6 2.2 6.8 7.0 19 04-05-11 100 33.0 31.8 4.5 5.6 1.9 5.7 6.9 20 05-03-11 100 30.9 31.7 6.7 5.6 2.5 8.1 7.0 Note: CV = Coefficient of var i ation for contro l reproduction. Lower CV bound determined by US EPA (JOih percent ile)= 8.9%. Upper CV bound determined by US EPA (901h percentile)= 42% MS D = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum pe r cent difference between the control and treatment that can be declared statistically significant in a who le effluent toxicity test. Lower PMSD bound determined by USE PA (IOih percentile)

13%. Uppe r PMSD bound determined by USEPA (901h percentile)

47%. CT = Central Tendancy (Mean Contro l Reproduction, CV, o r PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Un der the Na ti onal Poll utant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protect i on Agency, Cincinnati, OH. USEPA. 200Ja, 2001b. Final Report: Interlaboratory Variabi l ity Study of EPA Short-term Chronic and Acute Whole E ffluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-0 1-0 04 and EPA-821-B-01-005. US Enviro nmental Protection Agency, Ci n cinnati, OH. Page 91 of 100 File: CdNaC1CR_050311.xlsx Entered by: J. Reviewed 1 l; 1 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaohnia dubia Dilution preparation informadon: NaCI Stock INSS number: .Stock preparation: 100 g NaCIIL: Dissolve 50 g NaCI in 500 mL Milli*O water. Dilution prep (mg/L) 600 800 1000 ' 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test Ol'l!anism source in,{ormation: Qrganism age: < 24-hours old Date and times organisms were born os-.t\ CJ'\\,. TC> \OZ.OO between: Culture board: t1-1.*1..S..*I\ A Replicate number: l 2J3J4JSI6J718 1 9 1 10 Culture board cup number: 1.\ S I i I 1\ I I I I I.t Transfer vessel information: oH-=t.ao s.u. Tempe_rature =-'l.o.&.'\ oc Average transfer volume (mL): Daily renewal information: Day 2 3 4 5 6 7 Date Test initiation and feeding, renewal and feeding, or termination time CdNaCICR#: Comments: Test information: Randomizing template color: Incubator number and shelf location: YWTbatch: Selenastrum batch: MHSW batch used Analyst Page I of6 -c:Hd...h '2.& \ o&.\o '1.\*1 \ o4t'1*& \ Control information: Acceptance criteria Summa_I'J'_ o.{Jest end]J()ints: %of Male Adults: 67 .. 7-day_LCso ) Nl'l() %Adults having 3ra Broods: /ttl7, NOEC &oo % Mortality: {')7. LOEC /OM Mean Offspring/Female: I 5. 0 offspring/female ChV gttc.t.t/ %CV: ... <40.0% ICzs (a Page 92 of 1 00 SOP A T14 -Exhibit A Tl4.l, revision 04-0 l-09 I ' 'I 'c.* I I I ...: I I I I I I I I I I I ' Species: Ceriodaphnia duhia CONTROL Day 1 1 Young produced 0 Adult mortality L,.. 2 Young produced 0 Adult mortality L 3 Young produced (")_ Adult mortality '-4 Young produced '"' Adult mortality s Young produced \i.. Adult mortality L 6 Young produced t"i Adult mortality L 7 . Young produced \\. 2 0 \....-0 \. a L '--,.,_ '-c \..... ll.. Total young produced Final Adult Mortality \..... '-X for 3ra Broods >C-Page2 of6 CdNaCICR #: l'J-0 Survival and Reproduction Data Replicate number 3 4 s 6 7 8 9 10 _C 0 0 Q_ 0 0 a_ () \.... \.... \.._ '-L L '-L-0 0 D 0 c 0 () (') L. L.. \.... \...... \...-L. \...... L.. ("') 0 0 0 _Q 0 0 0 '-\...... \.._ '--\...... '-'--\....... 5 '"" "" '{ \......, '-'-'-\...... L.... \'0 \'b \\ \"'l.. \'1. n .. \'t. 10 \..... '-'-L. \.._ '-L.. '-0 0 c D 0 c 0 0 \_' \_ \...... \..... \.... L '--L.. 1'-l -1\t:. '"' \0 ,, '2.(.

32. 30 '-'--\...... \..... \..... \._. i'-"' X. ')<._ >< >< >< Note: Adult mortaltty (L = ltve, D-dead), SB-spltt brood (smgle brood spltt between two days), CO-carry over (offspnng carried over with adult during transfer).

600 mg NCIIL a S . I urvzva an epro uc zo a a Replicate number Day 1 l 3 4 s 6 7 8 9 10 1 Young produced f) (') () 0 _Q_ 0 0 0 0 0 Adult mortality L '-'-\.._ L \...._ \_ L L.. L. l Young produced 0 D 0 0 0 0 0 0 0 0 Adult mortality \.. \.... L... \....-.._ L L.. L L L. 3 Young produced 0_ 0 0 0 c 0 0 a 0 Adult mortality L \._ L '-\..._ L L. L L L. 4 Young produced s "-l '-4. s "-{ "' "{ Adult mortaJity L-\...... \....... '-L.. L.. '-L L L.. s Young produced 10 ,,_ \l.. 10 l2 I \ I\ H \'L... Adult mortality \_ \_ L L \.... L L '-L L 6 Young produced c c () () () t) () tJ 0 0 Adult mortality L \..... L '-L L L L. L-L.. 7 Young produced \&. \5 1\o tS Total young produced 4i 32. ?,.C., 3() Final Adult Mortality \...... '-L L l l \. L L.. '--Note: Adult mortality (L-hve, D-dead), SB =split brood (smgle brood spht between two days), CO-carry over (offspnng carried over with adult during transfer). Concentration: % Mortality_:

07. Mean Offspring/Female:

% Reduction from Control: o.a?. Page 93 of 1 00 SOP AT 14 -Exhibit AT 14.1, revision 04-01-09 i I I I 1 If" l ' ' <'i J :: 'I I ' I I I I I I I I -I I I I I .. ) Species: Ceriodaphnia dubia 800 N CI/L m2 a Day 1 l I Young produced 0 0 Adult mortality L \..-2 Young produced 0 0 Adult mortality t.. L 3 Young produced 0 6 Adult mortality '-L 4 Young produced "' Adult mortality '-'-s Young produced \"l.. \0 Adult mortality '-'--6 Young produced ("\ () Adult mortality \. L 7 Young produced \'5 \C::. Total young produced :?>\ '2.& Final Adult Mortality \.... '--Page3 of6 CdNaCICR #: t'l. 0 S ' I dR d urvwa an epro uct1on D ata Replicate number 3 4 5 6 7 8 9 10 Q (\ 6 0 0 0 0 c '-\_ L.. L L L L... \._ 0 () Q _a a 0 0 c L \... '--'-L L L '-0 c 0 0 c 0 0 0 '--'-L '-'--'--'---'-'-\ '-\ s 4 '--l "' ..!. 3 \.......* '-\. L \..... '--\..-L 10 H lO I'L \0 10 10 L-'-L L '--L '--'-c () (J 0 c 0 _0 L '--\._ L L '-L '--\"'\ \0::, 1\. H. ,, 1'-t 15 3() 2& '2., 3\ \....... '-\ '--\....... \.... '-Note: Adult mortality (L-hve, D-dead), SB = spht brood (smgle brood spht two days), CO= carry over (offspnng carried over with adult during transfer). Concentration: % Mortality: 07 .. Mean Offspring/Female: .. 2!t5_ % Reduction from Control: a:z.1. 1000 mg NCI/L a d ti D t, S . I dR urvwa an epro uc on a a ReDiicate number . Day 1 2 3 4 s 6 7 8 9 10 1 Young produced G (\ (i () 1'1 () () () 0 () Adult mortality L L L L \.._. L L L L L-2 Young produced c 0 c 0 (") a 0 Q 0 0 Adult mortality '--\._ \...... '--\...... \.._ L. \.... L L 3 Young produced ('} () (') () 0 0 () 0 0 a Adult mortality L L L L L L L '-L L 4 Young produced ¢ "" '-l 3 s 3 3 Adult mortlllity L L \..... '-L L. L '-L L s Young produced 10 11.. L'l.. It) /0 ct II IC 10 Adult mortality L \.._ L L L L L L L L 6 Young produced Q 0 G () () (') 0 (\ 0 0 Adult mortality \ L L L \ L-L L L-'--7 Young produced \\.. \S. \'5 t'-l 1'-l '"" I'S fC..( Total young produced '2.(\ .3b 2.C. 2.1 1..8 "2.1 '2.(. .3b *u 2.1 Final Adult Mortality '-'-L... '-L.. L L L.. '-'-Note: Adult mortahty (L-ltve, D-dead), SB = spht brood (smgle brood split between two days), CO-carry over {offspnng carried over with adult during transfer). emale: Page 94 of 1 00 SOP A Tl4 -Exhibit A T14.l, revision 04-01-09 I ' ' ' I c ' ' I ,r ;*

  • I I I I '

Species: Ceriodaohnia duhia 1200 N CI/L mtt a Day 1 l Young produced () Adult mortaUty L-2 Young produced D Adult mortallty '-3 Young produced 0 Adult mortality \..... 4 Young produced "l. Adult mortality '--5 Young produced a Adult mortality \..... 6' Young produced c Adult mortality L 7 Young produced Total young produced 1'\ Final Adult Mortality " 2 0 L. C) L 0 '--'-s \._ 0 L A 1\o '-CdNaCICR #: ll.O S . aJ dR UTVIV an epro uctzon D ata Replicate number 3 4 5 6 7 8 9 10 0_ 0 0 0 a 0 G a '--L.. L L.. L. L.... '-L.... Q (') _C c a ("'} L L L.. L.. L.. L '--\... 0 D Q c 0 0 0 0 \.... '-'--L... '-L. \..,. '--'-\ \ 3 "1.. '1... '-( L...* \..... '--'-\..... L. \..... '-., \Q '-\ "l , '-L.. '-\...... '-'--'--'-0 0 0 0 0 0 c 0 L \._ \....... L. L.. '-L. '-la 5 0 '"" , 1"\ I"( l2. , \l 18. '-'-'-'-L '--'--Note: Adult mortal1ty (L-live, D-dead), SB =split brood (smgle brood split between two days), CO-carry over (offspnng carried over with adult during transfer).

  • Concentration:

% Mortality:

07. Mean Offspring/Female:

% Reduction from Control: S1.0f. 1400 mtt NCIIL a S . I dR d t' D t urvzva an epro uc wn a a Reulicate number Day_ 1 2 3 4 5 6 7 8 9 10 1 Young produced (") 0 0 (\ () () 0 0 Q 0 Adult mortality \.... \.... \.... \._ L. L \...... L L L... 2 Young produced 0 0 0 0 0 6 0 0 0 0 Adult mortafity L L L L. L '-'--L.. \..._ L.. 3 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L.. L. L. \.... L L.. '-L L 4 Young produced I ("} D ' 0 0 c 7... 0 0 Adult mortlrlity \..... \..... '-'-L-'-'-\.... \.......... \....: s Young produced 0 '2. \ 0 "L l.. () 0 \ Adult mortality L.. L. \.... L L.. L L L L l 6 Young produced .3_ 0 c 0 (') 0 ..3 0 0 tJ Adult mortality _\....... L L. L \.._ L L., L L. 7 Young produced '-\ \ 0 L. 2... l s Q 3 Total young produced 8 3 \ '{ '-( "4 , \ (o F1nal Adult Mortality L '-L L L L \..... L.. L \....... Note: Adult mortality (L =live, D =dead), SB -split brood (smgle brood split between two days), CO-carry over (oftspnng carried over with adult during transfer). Page 4 of6 Page 95 of 1 00 SOP AT 14 -Exhibit AT 14.1, revision 04-01-09 E--'-T:r S-*;<,, ...... ::**** ... *: _)

  • J Environmental Testing Solutions, Inc. Control Day Replicate number I 2 3 4 s 6 7 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 4 4 3 5 4 4 6 5 12 12 10 13 11 12 12 6 0 0 0 0 0 0 0 7 16 16 13 14 16 16 15 Total 32 32 26 32 31 32 33 600 mg NaCIIL Day Replieate number I 2 3 4 5 6 7 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 5 3 3 4 3 4 5 5 13 10 12 12 10 12 II 6 0 0 0 0 0 0 0 7 18 15 15 16 13 17 15 Total 36 28 30 32 26 33 31 800 mg NaCIIL Day Replieate number I 2 3 4 5 6 7 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 4 3 4 4 5 4 4 s 12 10 10 II 10 12 10 6 0 0 0 0 0 0 0 7 15 15 17 15 13 16 16 Total 31 28 31 30 28 32 30 Page 96 of 100 ), Verification of Ceriodaphnia Reproduction Totals 1000 mg NaCIIL 8 9 10 Total Day 1 2 3 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 4 4 3 41 4 3 3 4 12 12 10 II6 s 10 12 9 0 0 0 0 6 0 0 0 16 13 17 152 7 16 15 13 32 29 30 309 Total 29 30 26 1200 mg NaCIIL 8 9 10 Total Day I 2 3 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 4 4 4 39 4 2 3 3 II II 12 114 5 8 5 5 0 0 0 0 6 0 0 0 15 16 15 !55 7 9 8 6 30 31 31 308 Total 19 16 14 1400 mg NaCI/L 8 9 10 Total Day 1 2 3 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 4 3 3 38 4 I 0 0 10 10 l3 108 5 0 2 I 0 0 0 0 6 3 0 0 17 14 15 153 7 4 I 0 31 27 31 299 Total 8 3 1 Replicate number 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 4 3 3 3 12 10 10 9 0 0 0 0 13 15 14 14 29 28 27 26 Replicate number 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 4 3 I 3 7 4 6 4 0 0 0 0 3 5 0 6 14 12 7 13 Replieate number 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 2 2 0 0 0 0 3 4 2 2 I 5 4 4 4 ....... : ' . -' ,_.......,.__,,_".

.... 8 0 0 0 5 II 0 14 30 8 0 0 0 2 5 0 4 II 8 0 0 0 2 0 0 5 7 9 10 Total 0 0 0 0 0 0 0 0 0 3 3 34 10 10 103 0 0 0 15 14 143 28 27 280 9 10 Total 0 0 0 0 0 0 0 0 0 2 4 27 4 7 55 0 0 0 3 7 51 9 18 133 9 10 Total 0 0 0 0 0 0 0 0 0 0 0 4 I 3 II 0 0 6 0 3 22 I 6 43 File: CdNaCICR 050311.xlsx Entered by: J. Sumner Reviewed by: cJ

  • * * *.::J _) r Environmental Testing Solutions, Inc. Conce ntrati on (mg/LNaCI)

I 2 3 Co ntr ol 32 32 26 600 36 28 30 800 31 28 31 1000 29 30 26 1200 1 9 1 6 1 4 1400 8 3 I Dunnett's MSD value: 2.511 PMSD: 8.1 Ceriodaplmia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test n11mbu: CdNaOCR IH 20 ------------------

Test dates: May 03-10 , 2011 Replicate number Sun ivai Average reproduction Codfociartor Ptrc:eat red11ct ioa fr o* 4 5 6 7 8 9 10 W*l (offspring/female) nriatioa (%) ooo trol ('Y o) 32 3 1 32 33 32 29 30 100 30.9 6.7 Not a pplicable 32 26 33 31 30 3 1 31 100 30..8 8..8 0.3 30 28 32 30 31 21 31 100 29.9 5.6 3.2 29 28 21 26 30 28 27 100 28.0 5.3 9.4 14 12 7 13 II 9 18 100 13.3 28.4 57.0 5 4 4 4 7 I 6 100 4.3 53.8 86.1 MSD = Minimum Significant Difference PMSD = Peroent Minimum Significant Difference PMSD is a measure oftest precision.

The PMSD is the minimum pcroent difference between the control and treatment that can be declared s tatis t ically significan t in a whole effluent toxic i ty test. Lo w cr-PM SD b9 und determined by USEPA (10 111 percentile) = 13%. U p pe r PMSD bound d etermined by US EPA (90tb p ercen tile)= 47%. Lower and upper PMSD bounds were determined fro m the l Oth and 90th percent il e, r espect ivel y. ofPMSD data from EPA's WET Int erlaboratory Variability S tu dy (USE P A., 2001a; USEPA., 200 I b). USEPA 200 1 a, 200 1b. Final Rcpon: Interlaboratory Variability Study o f EPA Sho rt-term Chronic and Acute Wh o l e Efflue nt Tox i city Test Methods. Volumes l and 2-Appcndix. EPA-821-B-01-004 and EPA-82 1-B-0 1-005. US Environmental PrOicction Agency. Cinc inn ati , O H. Page 97 of 1 00 F i le: CdNaC IC R_ 050311.XIsx Tab le populated fro m associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheet. Spreadsheet entered by: J. Reviewed by:

j. ,, *Ers * * * *
  • Statistical Analyses u Y, *... ,,.,.-.,.:
  • .. --'/:: .....

,) Environmental Testing Solutions, Inc. Cerioda2hnia Survival and Reeroduction Test-RS!roduction Start Date: 5/3/2011 TestiD: CdNaCICR SampleiD: End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: Comments: 2 3 4 5 6 7 8 D-Control 32.000 32.000 26.000 32.000 31.000 32.000 33.000 32.000 600 36.000 28.000 30.000 32.000 26.000 33.000 31.000 30.000 800 31.000 28.000 31.000 30.000 28.000 32.000 30.000 31.000 1000 29.000 30.000 26.000 29.000 28.000 27.000 26.000 30.000 1200 19.000 16.000 14.000 14.000 12.000 7.000 13.000 11.000 1400 8.000 3.000 1.000 5.000 4.000 4.000 4.000 7.000 Transform: Untransformed Conc-mg!L Mean N-Mean Mean Min Max CV% N t-Stat D-Control 30.900 1.0000 30.900 26.000 33.000 6.728 10 600 30.800 0.9968 30.800 26.000 36.000 8.766 10 0.091 800 29.900 0.9676 29.900 27.000 32.000 5.563 10 0.911 *1000 28.000 0.9061 28.000 26.000 30.000 5.324 10 2.641 *1200 13.300 0.4304 13.300 7.000 19.000 28.366 10 16.027 *1400 4.300 0.1392 4.300 1.000 8.000 53.763 10 24.223 Auxiliary Tests Statistic Kolmogorov D Test indicates normal distribution (p > 0.01) 0.82175648 Bartlett's Test indicates egual variances <2 = 0.07) 10.1990776 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp Dunnett's Test 800 1000 894.427191 2.51109516 0.08126522 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95%CL Skew IC05 857.368421 101.848477 536.115476 1000.42718 -1.5395 ICIO 1002.58503 37.7430109 902.799145 1019.00076 -1.1222 ICI5 1023.60544 9.16420366 1004.92346 1039.04671 0.1840 IC20 1044.62585 8.95991641 1027.21063 1060.90996 0.1797 IIC25 1065.64626 9.15526908 1047.78147 1082.77122 0.19211 1C40 1128.70748 11.7423916 1107.58171 1149.34211 0.4319 IC50 1170.7483 14.442622 1144.89854 1199.51128 0.5658 Page 98 of 1 00 REF-RefToxicant NACL-Sodium chloride CD-Ceriodaphnia dubia 9 10 29.000 30.000 31.000 31.000 27.000 31.000 28.000 27.000 9.000 18.000 1.000 6.000 !-Tailed Isotonic Critical MSD Mean N-Mean '30.900 1.0000 2.287 2.511 30.800 0.9968 2.287 2.511 29.900 0.9676 2.287 2.511 28.000 0.9061 2.287 2.511 13.300 0.4304 2.287 2.511 4.300 0.1392 Critical Skew Kurt 1.035 -0.1981616 0.65572311 15.0863171 MSB MSE F-Prob df 1279.06667 6.02962963 3.3E-34 5, 54 File: CdNaCICR 0503Il.xlsx Entered by: J. Swnner Reviewed by:+ I l I I I I I I I I I I I I I I I I I I I ;!) .J "Species: Ceriodaphnia dubia CONTROL 600 mg NaCVL 800 mg NaCVL STOCK Page 99 of 1 00 Page 5 of6 CdNaC I CR #: \2..0 SOP A Tl4-Exhibit A Tl4.1, revision 04-01-09 I I I Page 6 of6 Species: Ceriodaphnia dubia CdNaCICR #: \ 'Z.O CONTROL 600 mg NaCI/L i 800 mg NaCI/L I 1000 mg NaCI/L I I I I I Page 100 of 100 SOP A T14 -Exhibit A TI 4.1, revision 04-0 I-09 PERMITTEE NAM E/A DDRESS (In c lude Facility Name/loca tion if Different) Address_p ...Q . __________ _ _ _ _ _ CIN TEROFFICE OPS*SN-SQNI ______ _ _______ _ ___ _ _________ _ NATIONAl POUUTANT DISCHARGE E LI MINA TIO N SYSTEM (NPOE$) DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) EFFLUENT Form Ap proved. OMB N o. 2040-0004 ATTN: stephanie A. Howard 1 Y EAR MD r&%!-TriNG M llool...+-' 0 ...... NO D I SCHARGE D .... NOTE: Read instructions before this loon PARAMETER X Q U ANTITY OR LOADING QUAlfTY OR CONC E NTRATlON NO. FREQUENCY SAMPLE EX OF TYP E AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE *"****'***

                • 7 ******** 8 0 13/31 GRAB -12 MEASUREMENT 00400 1 0 PERM IT ******** ******** ** 6 **'******

9 su THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** 13 14 0 2131 GRAB -19 MEASUREMENT 00530 1 0 PERMIT ***'****" *"****** -*****"*** 30 100 MGfl TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH OIL AND GREASE SAMPLE ******** ***'*****

                • <6 <6 0 2131 GRAB -19 MEASUREMENT 00556 1 0 PERMIT ******** ******** -******** 15 20 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 0.985 1.303 ******** ............
  • '*******

0 31/31 RCORDR 03 ... TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD *******fr

      • lr**:lr:*
                • .. SEE RCORDR EFFLUENT GROSS REQUIREMENT MOAVG CAlLY MX PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT I NAME/TinE PRINCIPAL EXECUTIVE OFF I CER I Certify uncles penally or law lhal this doa.ment and al l attadlments wem prepared uncles my n TE L EPHONE DATE direction oc supervision in acoordance with a system desig1ed t o assure that qualified personne l Michael D. Skaggs property galher and evaluate the infonnalion S<bmitted.

Based on my inquiry or the person oc Sequoyah Site Vi JZ id ent persons ..t1o manage the system, a< those persons directly responsille fa< gathering the 423 843-7001 11 06 07 information, the information submitted is

  • to the best or my knowledge and belief , true , a crurate, Sequoyah Site Vice President and canplete.

I am aware lhat there are significant penalties roc sU:Jmitting false infonnation, SIGNATURE OF PRINCIPAL EXECUT IV E I including the possibility or fine a nd imprisonment for J<no..;ng viola lions. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TIPED OR PRINTED COMMENTS AND EXPLANATION OF ANY V I OLATIONS (Reference all att3chments here EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 PERMITIEE NAMEJADDRESS (lnclvde Facility NameA.ocation i f Different) _P ....Q * ..!i..OX 2JXIQ_ __________ _ _ _ _ _ ___ _ NATIONAL POLLUTANT DISCHARGE EU M INATION SYSTEM (NPDESJ DISCHARGE MONITORING REPORT (OMR) MAJOR (SUBR 01) EFFLUENT Fonn Approved. OMB N o. 204()..()()04 ATIN: stephanie A. Howard I Y EAR I MO 05 r:!T YRING [ 01 ] To IL-=-1..:....1 _J "** NO DISCHARGE I XX I ... PARAMETER X OUANTTTY OR LOADING AVERAGE MAXIMUM UNITS M I NIMUM TEMPERATURE , WATER DEG. SAMPLE ***"***** ,._ ..... .......... -CENTIGRADE 00010 1 0 PERMIT ******** ******** -******** EFFLUENT GROSS VALUE REQU I REMENT !T EMPERATURE , WATER DEG. SAMPLE ******** *****"*** ******** -CENTIGRADE MEASUREMENT 00010 z 0 PERMIT ******** ........... -***"**"* INSTREAM MONITOR I NG REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & SAMPLE *'*'****** ******** ******** ** UPSTRM DEG.C MEASUREMENT 00016 1 0 PERMIT ........ *--............ EFFLUENT GROSS VALUE REQUIREMENT FLOW , IN CONDUIT OR THRU SAMPLE ............

                • 03 TREATMENT PLANT MEASUREMENT 50050 1 0 P E RMIT ******** Req. Mon. MGD ******** EFFLUENT GROSS VALUE REQUIREMENT DAILYMX CHLORINE , TOTAL RESIDUAL SAMPLE ******** ******** *******'* ** MEASUREMENT 50060 1 0 PERMIT .........
  • '*******

-***'****'* EFF LUENT GROSS VALUE REQUIREMENT TEMPERATURE-C , RATE OF SAMPLE ******** *'******'* 04 CHANGE MEASUREMENT 82234 1 0 PERMIT ******** 2 DEGC ........ EFFLUENT GROSS VALUE REQUIREMENT DAILYMX SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEITffiE PRINCIPAL EXECUTIVE OFFICER I Certify t.f1der penally of law lhat tris document and all attaclmenls were prepared lA'lder my ir ection or supervision in aoc:ordance wi1h a system designed t o assue lhat qualified Michael D. Skaggs property gather and evaluate the infonna6on Slbnitted. Based on my inqoMy of the person or persons who manage the system. or those persons directly responsi>le for gathering the infonnation. the infonnalion submitted is. lo the besl of my knowledge and belief. true. accurate. Sequoyah Site Vice Pre sident land complete. I am aware that 1here are signifteanl penalties lor submitting false infonnalion. includi<19 the possibilrty of fine and irq>risonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No Discharge th is Pe r iod EPA Fonn 3320-1 (REV 3199) Previous editions may be vsed NOTE: Read instructions before complelinQ this fonn QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNIT S ANALYSIS ******** 04 ......... REPORT DEGC CO NT IN CALCTD DAILYMX uous ******** 04 ******** 30.5 DEGC CONTIN CALCTD DAILYMX uous **"***"*** 04 ******** 5 DEGC CONTIN CALCTD DAILYMX uous .............

                • .. ******** ******** .. CONTIN RCORDR uous 19 0.1 0.1 MGIL Five per CALCTD MOAVO DAILYMX Week ******** ****'****

.. . ......... ******** .. CONTIN CALCTD uous TELEPHONE DATE 423 843-7001 11 06 07 SIGNATURE OF PRINCIPAL EXECUTIVE I OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Faci li ty Name/toea/ion if Different) _________ _ ___ _ _________ _ NATIONAl POU.UTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SU BR 01) EFFLUENT Form Approved. OMB No. 2040-0004 MO ATIN: stephanie A. Howard 05 r%TvT9RING [ 01 ] To IL...:..1 -=-1 ..... NO DISCHARGE I XX I ..... NOTE* Read instructions before complelill!llhis form. PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TIPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANAlYSIS IC25 STATRE 7DAY CHR SAMPLE ***"*****

        • "****
                • ******** -23 CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 PERMIT **"***** .........

-43.2 *******:I-

    • "****'**

PERCENT SEM I COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** *******"'*

                • -23 PIMEPHALES MEASUREMENT lf RP6C 1 0 0 PERMIT ******** ******** -43.2 ******** .............. PERCENT SEMI COMPOS EFFLUE NT GROSS VALUE REQUIREMENT MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREME NT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT I NAME/TlTLE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penafty of law thai this docunent and al l attaclvnenls were prepared under my TE LE PHONE DATE direction 0< supeMsion in ac:ooroanoe with a syslem designed to assure tha t qualified personnel Michael D. Skaggs propelly gather and evaluate lhe inlonnation StbniUed.

Based on my inq<My of the person or persons who manage the system, << those persoos responsible for gathering the .rlormation , the lllformation so.bnitted 1s

  • to the besl of my and bebef, true , acrurate, 843-700 1 11 06 07 42 3 I Sequoyah Site Vice Pre sident and complete. I am aware that !here a1e signifocant penalties f O<

false infonnabon, SIGNATURE OF PRIN CIPAL E XECUTIVE inclucMg the possib ility of IWle and n.>risonment for knowi1'1ll violations. TIPED OR PRINTED COMMENTS AND E XP LANATION OF ANY VIOLATIONS (Reference all attachments her E No D is charge this Period EPA Form 3320-1 {REV 3/99) Previous editions may be used OFFICER OR AlJTHORIZED AGENT NUMBER YEAR MD DAY Page 1 of 1 PERM ITTE E NAM E/ADDRESS (Includ e Fa cili ty N a me/loca t ion if D i fferent) NATIONAL POL l UTANT DISCHARGE EliMINATIO N SYSTEM (N P OES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) F o nn Ap p r ov ed. OMB N o. 2 040-0004 Address _p.O._!!_OX 2000 ___________ _ ____ UNTEROFFICUlPS

  • SN*SQNl ______ _

_______ _ I & sToRM wATER ___ _ _________ _ I Y EAR I MO 05 EFFLUENT *"* NO DISCHARGE I XX I *-NOTE: Read instru cti ons befo r e co m ple li ll!l lhi s form ATTN: stephanie A. Howard PARAMETER X QUANTITY OR lOADING QUAliTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAX I MUM UNITS ANALYS1S OXYGEN, DISSOLVED (DO) SAMPLE ******** ******** ******** **'****** MEASUREMENT -19 00300 1 0 PERMIT "******* ******** ...... 2 ******** *******"" MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS , TOTAL SUSPENDED SAMP L E ******** *'******* '********

                • ... 19 MEASURE M ENT . 00530 1 0 PERMIT ******** ******** ...... ******** ******** 100 MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX WEEK SOLIDS, SETTLEABLE SAMP l E ******** ******** ******** *****"***

-25 MEAS U REMENT 0 0545 1 0 PERMIT ******** 1hl***'*** -******** ***'***** 1 MUL ONCE/ GRAB EFFLUENT GROSS REQU I REMENT DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPlE **'******

                • ******** 03 ** !T REATMENT PLANT M EASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD '********
                • ******** . ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX BATCH SAMP LE M EASURE M ENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEAS U REMENT PERM I T REQ U IREMENT NAMEmn.E PRINCIPAL EXECUTIVE OFF I CER I C ertify under penalty of law lhallhis document and all attac:lvnen ts were pr e par ed under my ... TElEPHONE DATE or supervision in aooordance with a s y stem de s iglled to 3SSISe lha1 qualified personnel Michae l D. Skaggs prope r1y gather and evaluat e th e s ubmitled. Ba sed oo my inquiry of th e person or who ma nage the system , or those persons d i r ectly r esponsible for gathering lhe 423 11 06 07 i nformation.

the information subm i tted i s , t o the besl of my knowledge a nd bel i ef. true , Sequoyah Site Vice President f.nd complete. I am awa re lha llhere are signifocanl penalt i es f or s lbnW!mg fa lse infonnatioo. SIGNATURE O F PRINCIPAl EXECUTIVE I including lhe possibility of fine and im prisonment f or knowii1Q violations. O F FICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachm e nts her e Dur in g this r eport in g per i od , t h ere has been no fl ow f r om the Dredge Po n d other than t hat result in g from ra in fall. EPA Fonn 332G-1 (REV 3199) Pre viou s ed i tion s m a y be u s ed Page 1 of 1 REVIEW/CONCURRENCE SHEE T DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-May 2011 DMR ORGAN I ZAT I ON: E nvironmental DOCUMENT PREPARED BY: Brad Love DATE: 06/07/2011 CONCURRENCES Name R c Signature -Comment Date v N S.M. Love X S. A. Howard X D.A. Day X M.D. Skaggs X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose , logical in construction , and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency. S58 110711 800-NPDES CORRESPONDENCE July 11 , 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1 h Floor , L & C Annex 401 Church St r eet Nashvil l e , Tennessee 37243-1534

Dear Ms. Dana Waits:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JUNE 2011 Enclosed is the June 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I cerUfy under penalty of law that this document and a ll attachments were prepared under my direct ion or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system , or those persons directly responsible for gathering the information , the information submitted is, to the best of my knowledge and belief, true , accurate , and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincere ly, Site V i ce Pres id ent Sequoyah Nuclear Plant Enclosures cc (Enclosures)

Chattanooga Environmental Field Office D i v ision of Water Pollution Control State Office Bu ilding, Su it e 550 540 McCallie Ave nue Chattanooga , Tennessee 37402-2013 B. E. Brickhouse , LP 5U-C G. M. Cook, OPS 4A-SQN D. A. Day , POB 2A-SQN S. A. Howard , OPS 5N-SQN K. Langdon , POB 2B-SQN U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington , DC 20555 D. B. Nida , LP 5U-C A. A. Ray , WT 11A-K G. R. Signer , WT 6A-K M.D. Skaggs , OPS 4A-SQN K. M. Hodges (EDMS) LP 2V-C Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 July 11,2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement

& Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Dana Waits:

SEQUOYAH NUCLEAR PLANT-DISCHARGE MONITORING REPORT FOR JUNE 2011 Enclosed is the June 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) Fonn Approved. OMB No. 2040-0004 Addre...s_s_p....Q. ____ _ ______ _ ___ _ EFFLUENT ATTN: stephanie A. Howard *** NO DISCHARGE D *** NOTE: Read instructions before completinA this form. PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 41.7 0 30/30 RCORDR -04 CENTIGRADE MEASUREMENT 00010 1 0 PERMIT ******** ******** -******** ******** Req.Mon. DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT .. DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 28.7 0 30/30 MODELD -04 CENTIGRADE MEASUREMENT 00010 z 0 PERMIT ******** ******** -******** ******** 30.5 DEG.C. CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAILYMX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** 2 0 30/30 CALCTD -04 UPSTRM DEG.C MEASUREMENT 00016 1 s PERMIT ******** ******** --******** ******** 3 DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILYMX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** 1688 ******** ******** ******** 0 30/30 RCORDR 03 ** TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ******** ******** -CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** ...Q. 825--.,.. 1 9.968 1ie In 0 -M"/30 GRAB -19 MEASUREMENT o.o'Z..'f 0.031 1J:8.,/A/t 50060 1 0 PERMIT ******** ******** **** ******** 0.1 0.1 MG/l FIVE PER CALCTD EFFLUENT GROSS REQUIREMENT MOAVC DAILY MAX WEEK TEMPERATURE-C, RATE OF SAMPLE ******** 1 ******** ******** 0 30/30 CALC TO 62 ** CHANGE MEASUREMENT 82234 1 0 PERMIT ******** .2 DEG ******** ******** ******* . .... CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILYMX C/HR NUOUS ' SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TinE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the infonnalion submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the infonnation, the infonnalion submitted is , to the best. of my knowledge and belief, true, accurate, 423 I 843-7001 11 07 08 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false infonnation, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No closed mode operation. Veliger monitoring data is in included as an attachment. The following injections occurred:

1. Towerbrom 960 2. Floguard MS6236 (max. calc. cone. was 0.11mg/L-Iimit 0.2mg/L) 3. Biodetergent 73551 (max. calc. cone. was 0.06mg/L-Iimit 2.0mg/L) 4. Spectrus CT1300 (max. calc. cone. was 0.038mg/L-Iimit 0.050mg/L)

EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1 Mean# of Water Mean# of Water SUB NOTES:% Sample Date ZM/m3 %Settlers Temp.('C) Sample Date Asiatic Temp. ('C) LOCATION LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-ISV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB 02/08/2011 0 0 9 02/08/2011 0 9 1-25-545 MJW 02/15/2011 0 0 23 02/15/2011 0 23 1-25-545 MLW 02/22/2011 20 100 10 02/22/2011 0 10 1-25-545 PB 03/01/2011 0 0 11 03/01/2011 0 11 1-ISV-24-1236 PB 03/08/2011 0 0 11 03/08/2011 0 11 1-ISV-24-1236 WE 03/16/2011 22 0 11 03/16/2011 0 11 1-ISV-24-1234 MLW 03/23/2011 0 0 11 03/23/2011 0 11 1-ISV-24-1234 MLW 03/30/2011 0 0 12 03/30/2011 0 12 1-15v-24-1236 MLW 04/06/2011 18 100 15 04/06/2011 0 15 1-ISV-24-1234 HMW .04/08/2011 45 100 15.5 04/08/2011 0 15.5 1-1SV-24-1236 WAW/PB 04/20/2011 21 100 16 04/20/2011 0 16 1-1SV-24-1236 PB May 2011 No Samples Collected June 2011 No Samples Collected PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Address J....Q.JtOX 2000 ___________ _ ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01} EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: stephanie A. Howard *** NO DISCHARGE D *** NOTE: Read instructions before completing this form. PARAMETER X QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM IC25 STATRE 7DAY CHR SAMPLE ******** ******** Monitoring

    • CERIODAPHNIA MEASUREMENT Not Required TRP38 1 0 PERMIT ******** ******** -43.2* EFFLUENT GROSS REQUIREMENT MINIMUM IC25 STATRE 7DAY CHR SAMPLE ******** ******** Monitoring
    • PIMEPHALES MEASUREMENT Not Required TRP6C 1 0 PERMIT ******** ******** -43.2 EFFLUENT GROSS REQUIREMENT MIMINUM SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Michael D. Skaggs I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the QUALITY OR CONCENTRATION AVERAGE MAXIMUM ******** ******** ******** ******** ******** ******** ******** ******** information, the information submitted is , to the best of my knowledge and belief, trua, accurate, 1----,-----,------,---------1 and complete. I am aware that !hera are significant penalties for submitting falsa information, Sequoyah Site Vice President Sequoyah Site SIGNATURE OF PRINCIPAL EXECUTIVE 1-------------------jincluding the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND OF ANY £Reference all attachments here Toxicity was not sampled in June 2011. EPA Form 3320-1 (REV 3199) Previous editions may be used OFFICER OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL TELEPHONE DATE 423 843-7001 11 07 08 NUMBER YEAR MO DAY Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Address _p..Q. BOX 2.._ __________ _ ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMRJ MAJOR (SUBR 01) EFFLUENT Fonn Approved. OMB No. 2040-0004 ATTN: stephanie A. Howard *** NO DISCHARGE D *** NOTE: Read instructions before completing this fonn. PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ******** ******** 7 ******** 8 0 13/30 GRAB MEASUREMENT

    • 12 00400 1 0 PERMIT ******** ******** ** 8 ******** 9 su THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** 6 8 0 2/30 GRAB ** 19 MEASUREMENT 00530 1 0 PERMIT ******** ******** ** ******** 30 100 MGIL lWICEJ GRAB EFFLUENT GROSS REQUIREMENT MOAvo* DAILYMX-MONTH OIL AND GREASE SAMPLE ******** ******** ******** <6 <6 0 2/30 GRAB MEASUREMENT
    • 19 00556 1 0 PERMIT ******** ******** ** ******** 15 20 MGIL lWICEJ GRAB EFFLUENT GROSS REQUIREMENT MOAVO DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.044 1.186 ******** ******** ******** 0 30/30 RCORDR 03 ** TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD ******** ******** ******** .. SEE RCORDR EFFLUENT GROSS REQUIREMENT MOAVO DAILYMX PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

..... NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my .. ...... TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those parsons directly responsible for gathering the Sequoyah Site Vice President information, tha information submitted is , to the best of my knowledga and beliaf, true, accurate, 423 843-7001 11 07 08 and complate. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of line and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY OR PRINTED COMMENTS AND OF ANY VIOLATIONS £Reference all attachments here EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA__.:_ SEOUOYAH PLAN!_ __ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR 01) Form Approved. OMB No. 2040-0004 P...Q.BOX200Q_ __________ _ ___ ____ _ _________ _ ATTN: stephanie A. Howard PARAMETER X TEMPERATURE, WATER DEG. SAMPLE MEASUREMENT CENTIGRADE 00010 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE, WATER DEG. SAMPLE MEASUREMENT CENTIGRADE 00010 z 0 PERMIT INSTREAM MONITORING REQUIREMENT jrEMP. DIFF. BETWEEN SAMP. & SAMPLE MEASUREMENT UPSTRM DEG.C 00016 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE MEASUREMENT TREATMENT PLANT 50050 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 50060 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE-C, RATE OF SAMPLE CHANGE MEASUREMENT 82234 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT QUANTITY OR LOADING AVERAGE MAXIMUM UNITS MINIMUM ******** ******** ******** ** *********

                • ** ******** ******** ******** ******** ** ******** ******** -******** ******** ******** ******** ** *****"'**
  • "'"'"'**"'*

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Req. Mon. MGD ******** DAILYMX ******** ******** ******** -******** "'"'***"'** -******** ******** 04 ******** ******** 2 DEGC ******** DAILYMX NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of Jaw that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, Sequoyah Site Vice President and complete. 1 am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. nPED OR PRINTED COMMENTS AND OF ANY £Reference all attachments here No Discharge this Period EPA Fonn 3320*1 (REV 3/99) Previous editions may be used EFFLUENT *-NO DISCHARGE I XX I .-NOTE* Read instructions before completing this form QUALITY OR CONCENTRAnON NO. FREQUENCY SAMPLE EX OF ITPE AVERAGE MAXIMUM UNITS ANALYSIS ******** 04 ******** REPORT DEGC CONTIN CALCTD DAILYMX uous ******** 04 "'"'***"'"'* 30.5 DEGC CONTIN. CALCTD DAILYMX uous ******** 04 ****"'*** 5 DEGC CONTIN CALCTD DAILYMX uous ******** ******** ** "'***"'***

            • "'*

"* CONTIN RCORDR uous 19 0.1 0.1 MGIL Five per. CALCTD MOAVC DAILYMX Week ******** ******** ** ******"'*

                • "* CONTIN CALCTD uous I ... ,., .. .. TELEPHONE DATE 423 I 843-7001 11 07 08 SIGNATURE OF PRINCIPAL EXECunVE OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY Page 1 of 1 PERMITIEE NAME/ADDRESS (Include Facility NameA.ocation if Different}

Address 2Q9Q_ __________ _ ____ ______ _ ___ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} DISCHARGE MONITORING REPORT (DMR} MAJOR (SUBR01) EFFLUENT Fonn Approved. OMB No. 2040-0004 ATTN: stephanie A. Howard I r:rriNG MO DAY *** NO DISCHARGE I XX I *** NOTE: Read instructions before completing this fonn PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ******** -23 CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 PERMIT ******** ******** -43.2 ******** ******** PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** ******** ******** -23 PIMEPHALES MEASUREMENT TRP6C 1 0 0 PERMIT ******** ******** --43.2 ******** ********* PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT ... SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 11 07 08 Sequoyah Site Vice President and complete. I am aware that.there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE 1 including the possibility of fine and imprisonment for knowing violations. OR PRINTED COMMENTS AND EXPLANATION OF ANY (Reference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY Page 1 of 1 PERMITTEE NAME/ADDRESS (Include Facility NameA..ocation if Different) . NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR01) Form Approved. OMB No. 2040-0004 __________ _ ___ DISCHARG: I & sToRM wATER ___ _ ATTN: stephanie A. Howard I MQ MQ DAY EFFLUENT From I 06 [ 01 ] To !11 J 06 30 *** NO DISCHARGE NOTE: Read instructions before completing this form PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE ******** ******** ******** ******** ** 19 MEASUREMENT 00300 1 0 PERMIT ******** ******** **** 2 ******** ******** MG/l TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** ******** -19 MEASUREMENT 00530 1 0 PERMIT ******** ******** -******** ******** 100 MG/l TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX WEEK SOLIDS, SETTLEABLE SAMPLE ******** ******** ******** ******** -25 MEASUREMENT 00545 1 0 PERMIT ******** ******** **** ******** ******** .1 MUL ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** 03 ** TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD *****A:**

                • ********
  • ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TinE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted.

Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is

  • to the best of my knowledge and belief, true, accurate, 423 843-7001 11 07 08 Sequoyah Site Vice President and complete.

I am aware thetthere are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE 1 including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY OR PRINTED COMMENTS AND OF ANY (Reference all attachments here During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1 REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-June 2011 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 07/07/2011 CONCURRENCES Name R c Signature -Comment Date v N B.M. Love X S. A. Howard X D.A. Day X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.}}

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