ML13289A199

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SQN Annual Water Withdrawal Updates for January 2011
ML13289A199
Person / Time
Site: Sequoyah  Tennessee Valley Authority icon.png
Issue date: 02/09/2011
From: Skaggs M
Tennessee Valley Authority
To: Cromer P
Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
Shared Package
ML13289A109 List: ... further results
References
Download: ML13289A199 (175)


Text

{{#Wiki_filter:S58 110209 801 - NPDES CORRESPONDENCE February 09, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1h Floor, L & C Annex 401 Church Street Nashville , Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JANUARY 2011 Enclosed is the January 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond . Samples collected from 9/23/2010 through 1/29/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information , please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I cerlify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely,

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I I } ' Michael D.* Sk~ggs/ Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control ATTN : Document Control Desk State Office Building , Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc : B. E. Brickhouse, LP 5U-C A. A. Ray, WT 11A-K D. A. Day, POB 2A-SQN G . R. Signer, WT 6A-K S. A. Howard, OPS 5N-SQN M . D. Skaggs, OPS 4A-SQN K. Langdon, POB 2B-SQN B. A. Wetzel, OPS 4A-SQN D. B. Nida, LP 5U-C K. M. Hodges (EDMS), lP 2V-C

Tennessee Valley Authority , Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 February 09, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JANUARY 2011 Enclosed is the January 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 1/29/2011 have all yielded results below detection limits for oil and grease . If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, .1" -*/)1 I ! {. ..:; _.;

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( ,l ' Michael D. Mag;gs Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building , Suite 550 Washington , DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37 402-2013

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name_~A-SEQUOY~NUCLEA~UNT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-{)004 (SUBR 01) Addres_L ___E. ~BOX 2000 _ _ _ _ _ ____ __ _

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NAME/TITLE PRINCI PAL EXECUTIVE OFFICER I Cert,fy under penalty of law that th1s document and all attachments were prepared under my 1--'--' Michael D. Skaggs

                                      - - - - - direction or supervision in accordance with a system designed to assure that qualified personnel Sequoyah Site Vice President properly gather and evaluate the information submitted Based on my inauiry of the person or persons wno manage the system. or those persons directly responsible for gathering the In formation, the information submitted is , to the best of my knowledge and belief, true, accurate, r1.5.fSi..~'~

Sequoyah P resident 423 TELEPHONE 843-7001 11 DATE 02 07

                                                           ~n d complete I am aware that there are significant penalties for submiWng false information,             SIGNATURE OF PRINCIPAL EXECUTIVE                            I TYPED OR PRINTED

__ _ _ - --linclud*ng tt"le possibility of fine and imprisonment for knowing VIOlations OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: 1. CCW data 2. veliger monitoring data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2

DMR Attachment CCWData CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained. CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 1/19/2011@ 1110 <0.10 mg/1 1/21/2011@ 1244 ND EPH

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Temp. ("C) Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp. ("C) LOCATION Clams/rri3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-ISV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB

PERMITIEE NAME/ADDRESS (Include Facifitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved Name_~A-SEQUOY~NUCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-{)004 (SUBR 01) AddreS£.._ _f.Q,__BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ -- - ~TffiOFFICESB~~Q~ - -- -- - -- TN0026450 F *FINAL -- - ~DDY - MISY . T~7~L _______ _ PERMIT NUMBER DIFFUSER DISCHARGE Facllitv_ _IVA_- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Loca.J!O.!:L .J:!AMILTON COUNTr._ _ _ _ _ _ _ _ _ _ _ EFFLUENT From

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ATTN : Stephanie A. Howard NOTE: Read instructions before completing this form. PARAMETER TITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY! SAMPLE EX OF TYPE ANALYSIS MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS _I CHLORINE, TOTAL R ESIDUAL **"****** I ** ******** I 0.019 t i .038 I 19 0 I 10 I 31 GRAB 50060 1 0 0 I PERMIT "**""**** - - ...-.;;.;** --~ _,.. ******** 0.10 . 0~1*0*--4 MG/L - + wEEK* CALCTD EFFLUENT GROSS VALUE 1TEMPERATURE

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NAME!TITLE PRINCIPAL EXECUTIVE OFFICER TELEPHONE DATE dlrectooo or supervision tn accordance wllh a S)l5tem destgned to BSSure that Qualified persoonat Michael D. Skaggs !property ga ther and evaluate tho tnfQilllatt on subm*tted Based on my lnQu*ry or the person or 1 1persons who manage me sy5tem, or those persons directly responsible lor gat.hering the Sequoyah Site Vi~ resident

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TYPED OR PRINTED includrng lhe possrbrhty of fine and rmprrsonment for knowong vrolalrons COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) OFFICER OR AUTHORIZED AGENT AREA CODE DAY The only B/CTP chemical injection this period was Towerbrom 960. EPA Form 3320*1 (REV 3/99) Previous editions may be used Page 2 of 2

PERMITIEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUO~~UCLEMPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040.{)004 (SUBR 01) Addres_L ...f. ~BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ - - -~TEROFFICESB-2A-SQ~--------

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G REOUIREMEI NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certoly underoenally of law that thos documem ana all altachmentli were prepared under my

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TELEPHONE I DATE Michael D . Skaggs properly gal her and evaluau~ the mlormat1on submtlted Based on my Inquiry of the person or 1 / \ persons who menagelhe s)'!lem. or those persons dlrOOily respon5ible lor gatherong the onformatoon, lhe mlormallon subm11ted ts . lo the besl of my ~nOWiedge and behel, true, accurate, Sequoyah Site "-i e President __ -~--=-'----........ 423 843-7001 11 1 02 07 Sequoyah S 1te V1ce President TYPED OR PRINITED and complete 1am aware 1ha1lhere are sogntfooant penalties for submottmg false onformatoon , _ j i ncluding Ihe oosS<biloly or fine and lmprosonment for knowmg violations. I SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA I. CODE T NUMBER YEAR MO I DAY COMMENT$ AND EXP~NATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in January 2011 . EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved . Name_~A-SEQUO~~UCL~~~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres_L __E.Q.,_BOX 2000 _ _ _ _ _ ___ ___ _ F- FINAL ---~mEROF FICESB-2A~Q ~--- - --- - -- - ~DDY - DAISY . T~7~L __ __ __ _ _ LOW VOL. WASTE TREATMENT POND Facili~~A-SEQUO~HNUCLEARPLAN~ -- -- EFFLUENT Locati~~AMILTONCOUN~ -- --- -- -- - From

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PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name_~A-SEQU~A~UCLEAAPUNT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres.§__ _f.Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A-SQ~- - ---- - - ---~DDY-DAISY~~7~L __ ____ _ _ TN0026450 I F- FINAL METAL CLEANING WASTE POND Faciliw_~A-SEQU~AHNUCL~RPLAm_ _ _ _ _ _ EFFLUENT Locati~~AMILTONCOUN~ ----- - ---- From

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I L___ --'-- __ ] NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 11Cert1ly under penalty o.I taw that this document and all attachments were prepared under my

       *                                        -  dtrect10n or superviSIOn In accordance Wlth a system designed to assure that Qualified personnel I                                                                   TELEPHONE                     DATE Michael D. Skaggs                  properly gather and t~valua te the mfcmJallon submllwd Based on my I~Qutry of lhe person or persons who manage tt>e syslem. o< those persons directly responsible for gathering iJle                      Sequoyah Site :vicelEresident
                       .   .       .               infO<milhon. the 1nformation submme<t ts
  • to t/1e best of my knowledge and belief true, accurnte, 423 843-7001 11 02 I 07 1

Sequoyah S1te Vrce President and complete 1am aware lhallllere are sigmficant penalties Ia< submitllng false information. 1nclud1ng the poss1bllity of fine end ompnsoomenllor knOWing v.olat1ons I SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUl HORIZED AGENT t . TYPED OR PRINTED - - - -- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) CODE ,

                                                                                                                                                                                                      - - - - - " ..::.::;.:::::__;___ _ _ __J__

MO I DAY _ j __ _. _ _ _ _ _ , No Discharge this Period EPA Form 3320-1 (REV 3199) P~e~i~'(iseditions ,;ay be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name_~A-SEQUO~~UCLEAAP~T _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres_L_f.~BOX2000 - - - - - - - - - - - - TN0026450 F- FINAL ---~TEROFFICESB-2A-SQ~-- ------ ---~D~-DAISY~~7~L _ _ _ _ _ _ _ _ RECYCLED COOLING WATER Facility_ ..JYA- SEQUOYAH NUCLEAR PLANT_ _ _ _ _ _ Locatio.!l._ __!iAMILTON COUNTY_ _ _ _ _ _ _ _ _ __ EFFLUENT From

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           - -                - -- * - - - - dlrecllon or supeMsion In accordance with a system designed to assure that quahfied personnel Michael D. Skaggs                ~property gathl!f and evaluate the lniO!'ITIIItton subr111tted Based on my rnou*ry of the person or persons v.'ho manage the system. ot those perSons dlrectly responsible for gathenng the Sequoyah Stte Vtce Presrdent 1nformauon. the Information subm*tted Is . to the best cl my knowledge and belief. true. accurate.

land complete 1am aware that there are s*gmficant penalties lor submiWng false inlormabon,

                                                 -;*nclud*ng the possibility or fine and *mpnsontnel1t for knowtng Vloiahons I      SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423      843-7001       11 I 02* I 07 AREA   I  NUMBER        YEAR \ MO     I DAY TYPED OR PRINTED                                                                                                                                                                                           CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAMEJADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR Name_~A-SEQUOY~NUCLEARPLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-{)004 (SUBR 01) Addres_L _l'_.Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-2A-SQ~-------- TN0026450 110 T F- FINAL ---~DDY - DAISY~~7~--------- RECYCLED COOLING WATER Facili~~A-SEQUO~HNUCL~RPLAN~---- Locati~~AMILTONCOUN~----- - -- -- EFFLUENT NO DISCHARGE I XX I ... ATIN: Stephanie A. Howard From NOTE: Read instructions before comoletinQ this form

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__ j I I I_ I ___ NAMEJTITLE PRINCIPAL EXECUTIVE OFFICER Jl Cerlofy under penally of law that lhos dowment a~d all aUachmams were prepared und~r my TELEPHONE DATE

d~redlon or supeMSoon on accordance with a syslem c;!esogned tP assure lhat Quatofied personnel Michael D. Skaggs properly galher and evalual1! lhe Information submtted Based on my Inquiry of lhe persQ!l or
                                                 !persons who manage lhc system. or those persons dlreclly respooslble for galhenng the                        Sequoyah Site-<<L!Ce President
                      .   .      .               jtnformalion , the lnformatoon subl)ll tted Is . to lhe besl of my knowtedge and belief. lrue, accurale.                                                         843-7001        11 . 02      I 07 Sequoyah S1te Vice Pres1dent              and complele, 1am aware that the<e are slgnlflcanl penelloes for submi ttong fal se onrormation.         SIGNATURE OF PRINCIPAL EXECUTIVE

_ _ !oncludlng lhe possibility of r.ne and lmpnsonment for ~nowong vlola lions TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO I DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved . Name _ ~A-SEQUOY~NUCLEA~~T _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01} Addres_L _.E.Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TEROFFICESB-~-SQ~-------- TN0026450 I 116 G F- FINAL ---~DDY - DAISY~~7~L --- - --- - PERMIT NUM~ ___, DISCHARGE NUMBER BACKWASH Faciii~~A-SEQUOY~NUCL~RP~N~ --- - EFFLUENT L~li~~AMILT~COUN~ - - -------- I f¥10N!IqfillJ.G -,EE_RIPJ? . I . .. I ATTN : Stephanie A . Howard From

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              ---        - - - - - - - - - - ' d*rectlon or supervoslon In accordance woth a system desogned to assure that qualified personnel Michael D. Skaggs                     prcpeny gather and evaluate the tnfonnatooo submlt1ed Based oo my mqu*ry of the person or persons wl1o manage the system. or those persons dlreclly responsible for gothoring the
                     .    .         .                .nformabon. the Information submllled IS to the besl of my knowledge and belief. true. accurate,                                                                             843-7001        I 11 I 02         07 Sequoyah Stte Vtce Prestdent                .and complete. 1am aware tnat there are Significant penalbes for submo!tmg false information.

_ __ _ ___joncludmg the POSSibility of line and imprisonment for knOWing v!Oiabons NUMBER IYEAR I MO I DAY TYPED OR PRINTED COMMENTS AND EXP~NATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name _ _!YA

  • SEQUOYA~UCLEAR PLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01)

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                                                                                                                                                                            ***  NO DISCHARGE            D ...

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_j I I_ .L_L_J NAME/TITLE PRINCIPAL EXECUTIVE OFFICER II Certify under penalty of law lhat this document and all attachments were prepared under my TELEPHONE DATE Michael D . Skaggs

                                               ~direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the r~c?~Site6?~5resident Sequoyah Information, the information submitted is , to the best of my knowledge and belief, true, accurate.                                              423 I          843-7001      11      02     07 Sequoyah Site Vice President           and complete. I am aware that there are significant penalties for submitting false information.       SIGNATURE OF PRINCIPAL EXECUTIVE ln~uding  the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT             AREA _j         NUMBER      YEAR     MO     DAY TYPED OR PRINTED                  I                                                                                                                                               CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS               (Reference all attachments here)

Operations performs visual inspections for floating debris and oil and grease during all backwashes . EPA Form 3320..1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facililv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved MAJOR Name_~A-SEQUO~~UCLEA~LANT _ __ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 AddresL_f- ~BOX2000 - - - - - - - - - - - - ---~TEROFFICESB-2A~Q~ --- - --- - L. 118 G F- FINAL ---~DDY-DAISY~~7~L _ _ _ _ _ _ _ _ Facili~~A-SEQUO~HNUCLEARPLAN~ -- -- - I - . _....... **-***--** I WASTEWATER & STORM WATER Locatio.!l._ _!:!AMILTO.!i.COUNTY _ ___ __ __ __ _ EFFLUENT DAY

                                                                                                                                                                                                       ... NO DISCHARGE          I XX I ...

ATTN : Stephanie A. Howard From 31 NOTE: Read instructions before completinq this form. I PARAMETER QUANTITY OR LOADING r---------------------------~---------~~- OXYGEN , DISSOLVED (DO) SAMPLE AVERAGE~ MAXIMUM I UNITS

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l SAMPLE L MEASUREMENT PERMIT I REQUIREMENT -1 L._ ___j_ ___ i I I LI I I_-~_ _j l NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER II Cer111y under penalty of law tnat 1n1s document and all attachments wer13 P<eoa1ed under my TELEPHONE DATE

       ---                                          - 'direction or supe<v1t;1on 1n accordance With a system des1gned to assure that qw~ldied personnel Michael D. Skaggs                     proper1y gal her and evaluale lhe 1nformauon subm* tted Based on my 1nqu1ry of the person or l

persons v.Nl manage lhc system, or those persons directly responsible for galhenng lhe tnformat*on, the Information submitted Is , to lhe besl or my knowledge a!ld belief, true, accurate. ' £ 1 423 843-7001 11 I 02 I 07 Sequoyah Site Vice President a!ld complete. I am aware that there are slgmftcant penalties for subtn11ting false Information . -* *** .. - .. *-*- .. ----- .... *-" _j *nd ud ~ng the llOSS*blhty of fine and lmpnsonmentfQ( knowing v.OiaUoos YEAR MO I DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- January 2011 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 02/07/2011 CONCURRENCES Name R c Signature - Comment Date v N B.M. Love X S. A. Howard X D.A. Day X M.D. Skaggs X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 110308 800- NPDES CORRESPONDENCE March 8, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR FEBRUARY 2011 Enclosed is the February 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 2/26/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, ~:t~s Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure) : Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control ATTN: Document Control Desk State Office Building , Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 cc: B. E. Brickhouse, LP 5U-C D. B. Nida, LP SU-C G. M. Cook, OPS 4A-SQN A A Ray, WT 11A-K D. A Day, POB 2A-SQN G. R. Signer, WT 6A-K S. A Howard, OPS SN-SQN M. D. Skaggs, OPS 4A-SQN K. Langdon, POB 28-SQN K. M. Hodges (EDMS), LP 2V-C

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 March 8, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR FEBRUARY 2011 Enclosed is the February 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 2/26/2011 have all yielded resu lts below detection limits for oil and grease. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, ~~~Ls~ Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Wash ington , DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

PERMITTEE NAME/ADDRESS (Include Facility Name/location if DifferenO NATIONAL POUUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form ApProved. DISCHARGE MONITORING REPORT (DM R) ~m~-~~SEOUOY~~UCLEAAP~ -- - (SUBR 01) OMB No. 2040-0004 Mdre~ _p....Q.JlO.X.ZOO!L _ _ _ _ _ _ _ _ _ _ _ ---~INTER~~~B-~S~L __ _ ___ _ --- ~ODDY~AI~Wi~-------- ~dli~ -~~~~~~~~AR~ID _____ Lo~~~~~N~OU~---------- ATTN : stephanie A. Howard X PARAMETER OUANrrrY OR LOADING OUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM MINIMUM UNITS ANAlYSIS UNITS AVERAGE MAXIMUM TEMPERATURE, WATER DEG. CENTIGRADE SAMPlE MEASUREMENT

                                                                                                                         -              .............         .............           12.8         04        0     28 I 28   MODELD 00010      z      0     0                     PERMIT REQUIREMENT
                                                                      *****"***                  ********              -                ********              ********                30.5       DEG.C.             SEE      CKREQ INSTREAM MONITORING TEMPERATURE, WATER DEG.                      SAMPlE MEASUREMENT DAILYMX 25.3         04        0 PERMIT 28 1 28   RCORDR CENTIGRADE 00010      1      0     0                     PERMIT REQUIREMENT
                                                                                                                        ......          ********              ............         REPORT        DEG.C.              SEE      CKREQ EFFLUENT GROSS VALUE                                                                                                                                                               DAILY MX                        PERMIT TEMP. DIFF. BETWEEN SAMP. &

UPSTRM DEG.C SAMPlE MEASUREMENT

                                                                                                                         -               *"*"'******           ..............          3.5         04        0     28 I 28   CALCTO 00016     1       w EFFLUENT GROSS VALUE 0                     PERMIT REQUIREMENT
                                                                       ********                  ********              -**              *"*****                ********                5.0 DAILYMX OEG.C.           CONTIN uous CALCTD PH                                           SAMPlE MEASUREMENT 8.4               ********

8.6 12 0 4 / 28 GRAB 00400 1 0 0 PERMIT ******** ******** 6.0 9.0 su WEEKLY GRAB REQUIREMENT MINIMUM EFFLUENT GROSS VALUE SOUDS, TOTAL SUSPENDED SAMPLE ........... *******"* ... *****'*** 7 MAXIMUM 7 19 0 1/28 GRAB MEASUREMENT 00530 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT MOAVG 30 100 DAILY MX MGn.. MONTHL'I' GRAB OIL AND GREASE SAMPLE *******'* *******"*

                                                                                                                          -              ********                    <6                 <6                   0      1 I 28     GRAB MEASUREMENT                                                                                                                                               19 00556      1      0     0                     PERMIT                    "'*"'*****                *"*****"**             ****
                                                                                                                                          ********                  15                  20        MGn..           MONTHLY      GRAB REQUIREMENT EFFLUENT GROSS VALUE
                                                                                  ....                                                                        MOAVG                DAILY MX FLOW, IN CONDUIT OR THRU                     SAMPlE                                                1641                   03
                                                                                                                                                                .............        ********       ..        0     28 I 28   RCORDR iTREATMENT PLANT                         MEASUREMENT 50050      1      0     0                     PERMIT REQUIREMENT
                                                                        ..........              REPORT                 MGD                *******'*             ********             *******'*     -               CONTIN RCORDR EFFLUENT GROSS VALUE                                                                           DAILY MX                                                                                                             uous
                                                                                                                                                       '1.~~p~~***

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty or law lhallhis doaJmenl and all attaclwnents were prepared under my TELEPHONE DATE uio eclion << supervision in aa:orclance wilh a system designed to assu-e Ulal qualified per some! Michael D. Skaggs properly gather and evaluate lhe onlonnallon sullmrtted. Based on my inquwy or lhe per$011 or persons who manage lhe system.. << those persons diredJy responsible lor ga!h&ing lhe Sequoyah Site Vice President information. lhe lnlormation submotled is

  • to lhe best of my knaMedge and bebel, true, accurate, 423 843-7001 11 03 05 jand complete. I am aware Ulallhere are sognoficam penalties I<< submitting false inrormallon. SIGNATURE OF PRINCIPAL EXECUTIVE I ondudong lhe possib<lity or r.ne and omprisonment I<< knowing violations
~~ I TYPED OR PRINTED OFFICER OR Al.ITHORIZED AGENT NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOlATIONS (Reference all Jttachments h ere No closed mode operation. The following information is include<i in an attachment: 1. CCW data 2 . Veliger monitoring data EPA Form 332().1 (REV 3199) Previous editions may be used Page 1 of 2

DMR Attachment CCWData CCWTRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained. CCWCHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 2/9/2011 @ 1120 <0.10 mg/1 2/11/2011@ 1214 ND EPH

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp.fC) Temp.fC) LOCATION Clams/m3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-ISV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB 02/08/2011 0 0 9 02/08/2011 0 9 1-25-545 MJW 02/15/2011 0 0 23 02/15/2011 0 23 1-25-545 MLW

PERMITIEE NAME/ADDRESS (Include Facility Namell..ocation rf Different) NAnONAL POLLUTANT DISCHARGE EUMINAnON SYSTEM (N POES} MAJOR Foon APProved. DISCHARGE MONITORING REPORT (OMR) Na~--~~SEOUOY~~UCL~R~~~ --- (SUBR 01) OMB No. 2040-0004 Ad!L~_P..Q.Jl...OX.2~ __________ _ --- ~I~O~ ICESB-~-S~L ______ _ --- ~~ID~AI~rn~-------- ~dli~ -~~~~~~UC~RPWU_ _ _ _ _ w~o~A~TO~NTI _________ _ EFFLUENT

                                                                                                                                                                             * - NO DISCHARGE           D ...

ATIN: stephanie A. Howard NOT£: Read inslructioos before oomplelill!l this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TIPE CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT AVERAGE MAXIMUM UNITS MINIMUM AVERAGE 0.026 MAXIMUM 0.042 UNITS 19 0 ANALYSIS 11/28 GRAB 50060 1 0 0 PERMIT .........*** ******** ******** 0.10 0.10 MG/l WEEK- CALCTD REQUIREMENT EFFLUENT GROSS VALUE TEMPERATURE- C , RATE OF SAMPLE MEASUREMENT

                                                                           ********                      0                   62
                                                                                                                                             .........         MOAVG INSTMAX
                                                                                                                                                                                                        ..        0 DAYS 28 / 28        CALCTD CHANGE 82234      1    0   0                             PERMIT                    ******"**                      2                 DEG               *'*******         **'******           *'******           -                CONTIN          CALCTD EFFLUENT GROSS VALUE BORON, TOTAL REQUIREMENT SAMPLE MEASUREMENT DAILY MX                  C/HR
                                                                                                                                -             ********           <0.20             ...............      19        0 uous 4/29 1/qo~
                                                                                                                                                                                                                                       ~RAB
                                                                                                                                                                                                                                       ~It I
                                                                           .............                                     -                                                                                                         ~.GRAB
                                                                                                                                                                                                                         =~*=~ s/u 01022     1    0    0                            PERMIT                                              -****                                    ******'**        REPORT              ********            MG/l REQUIREMENT EFFLUENT GROSS VALUE SAMPLE                                                                                                                                                                QTF.L'(

MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

                                                                                                                                                        ~i'DIS~ p~~'"'

NAME/TITLE PRINCIPAL EXECUTNE OFFICER I CertJiy o..nder penany of law that thos doaJmenl Md all allac:lwnents were pr~ ln!er my TELEPHONE DATE direction or supervision in ac:axdaoce with a system designed to assure that qua rifted pe<sonnel Michael D . Skaggs property gather and evaluate lhe information submitted Based en my inquiry oflhe pe<son or persons who manage the system, or those persons cliredly responsible for galhenng the onfonnatJon. lhe informatJan submitted IS

  • to lhe best of my knawtedge and belief. true, aa:onte, 423 843-7001 11 03 05 Sequoyah Site Vice President and canplele I am aware that then! are sq,ifcanl penalbes for SLOmll.ng false informa6on, oncluding the possibility of fme and mprisonment for knowing violations..

SIGNATURE OF PRINCIPAL EXECUTIVE I

                                                                                                                                                                                                   ~~~ I OFFICER OR AUTHORIZED AGENT                       NUMBER          YEAR      MO    DAY TIPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS fReference all attachments here The following 8/CTP injections occurred this period: Biodetergent 73551 (max. calc. cone. was 0.02mg/L-Iimit 2.0mg/L)

EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Fad/ltv Name/Location if Differen t) NATIONAL PO U.UTANT DISCHARGE EUMINATlON SYSTEM (NPDES} MAJOR Form APProved. DISCHARGE MONITORING REPORT (DMRJ NamL_~~SEOUOY~~UCL~P~~ --- (SUBR 01) OMB No 2040-0004 .MCir~ _p...Q..!!.PX2J.l91L _ _ _ _ _ _ _ _ _ _ _ ---~IN~OF~UB*~*~L -- - -- -- --- ~ODDY~AI~rn37~-------- Fa~~ -~~EQ~~~~R~ID_ __ _ _ I--P-;.;:. ;.M.;~r; .02; .; ;.N64;u.. M;.5; . 0;s;ER. .-~11 olscHARGE 1

                                                                                                                                                                               ~~M:ER I ~~;~:~roRING              FoR ouTFALL 101 EFFLUENT LO~~ -~~ro~~----------

ATIN: stephanie A. Howard MD 02

                                                                                                                                                                                                ""* NO DISCHARGE           o *-

NOTE: Read instructions before oompleti~ this foon. X PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FR£QUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR CERIODAPHNIA SAMPLE MEASUREMENT

                                                                                                                                           -                  Monitoring Not Required
                                                                                                                                                                                                      ********            23 ITRP38     1       0    0                           PERMIT REQUIREMENT
                                                                            "*******                 ********                           -                        45.2            ***""*""             """**"""         PERCENT              SEE         COMPOS EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR                              SAMPLE MEASUREMENT MINIMUM Monitoring         ********             -                   23 PERMIT PIMEPHALES                                                                                                                                                   Not Required PERMIT

!RP6C 1 0 0 REQUIREMENT

                                                                            '*******'*               ********                                                    45.2            ********             ********         PERCENT              SEE         COMPOS EFFLUENT GROSS VALUE                                                                                                                                          MIMINUM                                                                     PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
                                                                                                                                                                          ~~~ ~~P~Ideot NAME/TinE PRINCIPAL EXECUTIVE OFFICER I Certify Ulder penalty of law lhallhls docl.wneR and al al1ac:hmenls were prepared under my                                                                                     TELEPHONE                   DATE diredJoo or supe<Vision in accordance ..mh a system d~ to assLQ lhat qualified personnel M ichael D . Skaggs                 property gather and evalua1e the infonnalion submllled. Based on my inquiry or the person or persons who manage the system, or those persons <Wectly cesponslble for ga1hering the tnformallon, the information Slbnilled is. to the best or my l<nowledge and belief, true. ac:cuate,                                                              423       843-7001        11       03    05 Sequoyah S ite Vice P~esident              and complete I am aware lhat there are signifocant penalbes lor Stbmitttng false tnlormabon.,

or includtng the poss;bility f1fle and omprisonment lor t<now;ng violations SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED OFFICER OR ALrrHORIZED AGENT

                                                                                                                                                                                                                     ~~~~ I     NUMBER       YEAR       MD   DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                  fReference all attachments here Toxicity was not sampled in February 201 1.

EPA Fonn 3320-1 (REV 3/99) Previous editions m ay be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Faolity N ame/location ff Different) NATIONAL POU.UTANT DISCHARGE EUMINATION SYSTEM (NPDES) MAJOR Fonn Approyed. DISCHARGE MONITORING REPORT (DMR} m~--~~SEOUOY~~UC~AAP~~ --- (SUBR01 ) OMB No. 2040-0004 M!tr.lill_P..Q.Ji0X2Jl90_ _ _ _ _ _ _ _ _ _ _ _ ---~ INTER~~ESB-~-S~L ------- --- ~~DY~~Y. Wi~-------- Fadi~ _WA~~~y~~UCLEARP~L _ _ _ _ I--P-:.;R. ;~.. ; ~T.;.o.;.2;N; ,64 u.;.. 50 eR----111 olscHARoe ~~M~ER I :~:':~. wAsTE M;;..s;;.. 1 TREATMENT PoNo LO~~ ~~~~~NTI_ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN: stephanie A. Howard

                                                                                                                                                                                     -* NO DISCHARGE           o -

NOTE: Read instructions befofe completing thiS form. L>< PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TIPE ANALYSIS PH SAMPLE MEASUREMENT AVERAGE MAXIMUM UNITS MINIMUM 7.0 .......... AVERAGE MAXIMUM 7.8 UNITS 12 0 12/28 GRAB 00400 1 0 0 PERMIT REQUIREMENT

                                                                     ...........                ********                               -                6.0             ********             9.0               su              THREE!         GRAB EFFLUENT GROSS VALUE                                                                                                                                 MINIMUM                             MAXIMUM                                 WEEK SAMPLE                                                                                                   ******"**                                                                  4 / 28         GRAB SCUDS, TOTAL SUSPENDED MEASUREMENT 75                        84                                26                                    9                 11               19        0 00530      1     0   0                       PERMIT REQUIREMENT 380                      1250                        LBS/DY                 *"it****"             30                100              MGR..           WEEKLY           GRAB EFFLUENT GROSS VALUE                                                 MOAVG                     DAILYMX                                                                  MOAVG            DAILYMX OIL AND GREASE                               SAMPLE                       <52                       <57                                 26            ********              <6                 <6               19        0       4 / 28        GRAB MEASUREMENT 00556      1     0   0                       PERMIT REQUIREMENT 190                       250                        LBS/DY                 ..........            15                 20              MGR..            WEEKLY          GRAB EFFLUENT GROSS VALUE                                                 MOAVG                     DAlLY MX                                                                 MOAVG             DAILYMX FLOW, IN CONDUIT OR THRU

~REATMENTPLANT SAMPLE MEASUREMENT 0.960 1.110 03 ******** ******** .........** ... 0 28 / 28 TOTALZ 50050 1 0 0 PERMIT REPORT REPORT MGD ******** ******** *****"***

                                                                                                                                                                                                                ..                SEE         TOTALZ REQUIREMENT EFFLUENT GROSS VALUE                                                 MOAVG                     DAlLY MX                                                                                                                          PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME!nTLE PRINCIPAL EXECUTIVE OFFICER M ichael D . Skaggs I Certify under penally of law lhat this dOOJrnent and all allachments were prepared under my direction or supetVision in aa:oniance wilh a system desogned to assu-e thai qualified personnel prope<1y gather and evakla:e the informallon submitted. Based on my inquiry of the pef'SOfl or persons who manage the system, or those persons directly responsille lor ga!hering the information. the onformalion subn1tted is
  • to the best of my knowledge and belief. true. accurate, r-1 ~s::1J '-,

Sequoyah President 423 TELEPHONE 843-7001 11 DATE 03 05 Sequoyah Site Vice President and complete I am aware that there are ~ndicanl penalties lor stbm1t1Jng false informaoon. SIGNATURE OF PRINCIPAL EXECUTIVE I InCluding lhe possibollly of f~ne and Wnprisonmenllor knowing violations TIPED OR PRINTED OFFICER OR AUTHORIZED AGENT

                                                                                                                                                                                                         ~~~I        NUMBER       YEAR       MO   DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS              l l?eference all attachments here EPA Fonn 3320-1 (REV 3199)        Previous editions may be used                                                                                                                                                              Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Faa7itv NarneA..ocation if Different) NATIONAL POU.liTANT DISCHARGE ELIMINATION SYSTEM (NPDES} MAJOR Fonn APProved. DISCHARGE MONITORING REPORT (DMR} ~m~-~~SEOUOY~* NUC~AAP~ --- (SUBR 01) OMB No. 2040.()(1()4 Mdre~_p..Q ...!l..Oll~ - _________ _ ---~INTE R~~U~S~L ---~OD~~AISY.m ~-------- 1---P-;-R-~-~-02-N645 -u -M_so_ER

                                                                                                                                 - ----tll DISCHARc;             ~~M~ER I ~~~:~LEANING                   wAsTE PoNo

£~1i~ -~~~~YA~~LEARP~N~--- EFFLUENT ~~tlo~~~~~~---------- pay 28

                                                                                                                                                                                    *~ NO DISCHARGE           I XX I m ATTN: stephanie A. Howard NOTE: Read instructions before completin!llhis fonn.

X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNTTS MINIMUM AVERAGE MAXIMUM UNTTS ANALYSIS PH SAMPLE MEASUREMENT

                                                                       ******"*'*               *******'*                  ..                                      ********                                   12 00400     1     0   0                          PERMTT REQUIREMENT
                                                                       **"******                *******'*                -                     6.0                 **"""'***                9.0               su               DAILY         GRAB EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED                        SAMPLE MEASUREMENT MINIMUM MAXIMUM 19 00530     1     0    0                         PERMTT 11nHI.          ................          ********                 30               MG.IL             DAILY        COMPOS REQUIREMENT EFFLUENT GROSS VALUE OIL AND GREASE                                 SAMPLE MEASUREMENT DAILYMX 19 00556      1    0    0                         PERMTT                    ********                 ********
                                                                                                                         .....           ********                  ***""**'**               15               MGIL              DAILY         GRAB REQUIREMENT EFFLUENT GROSS VALUE PHOSPHORUS, TOTAL (AS P)                       SAMPLE MEASUREMENT DAILYMX 19 00665      1    0    0                         PERMTT                    ********
                                                                                                 ...,...,.,.              .....           ******'**                 *****"*                 1.0              MGIL              DAILY        COMPOS REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                                                                    DAILYMX COPPER, TOTAL (AS CU)                         SAMPLE MEASUREMENT
                                                                         ********                **'******                  ...            **"'*****'*              ********                                  19 01042     1    0    0                          PERMIT REQUIREMENT
                                                                         ********                ********                  -              ***-*                     .............           1.0              MGIL               DAILY       COMPOS EFFLUENT GROSS VALUE IRON, TOTAL (AS FEI                           SAMPLE MEASUREMENT DAILYMX 19 01045     1    0    0                          PERMTT                    ...........              ****-**                                  ***-**"                   ********                1.0             MGIL               DAILY       COMPOS REQUIREMENT EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU                      SAMPLE MEASUREMENT                                                                       03
                                                                                                                                           ...............          -                   DAILYMX TREATMENT PLANT 50050     1     0 EFFLUENT GROSS VALUE 0                          PERMIT REQUIREMENT REPORT                     REPORT                   MGD               ********                  ********             .........           -                 DAILY        CALCTO MOAVG                   DAILY MX
                                                                                                                                                                              \

LL1~~*** NAMEmTLE PRINCIPAL EXECUTIVE OFFICER 1Cerufy Lnfer penalty or law 11\allhis doa.ment and aU atlachmenls were prepared under my TELEPHONE DATE direc:bon or supennsion in accc<dance Wllh a system designed to assure lhal qualifoed personnel Michael D. Skaggs property gather and evakJate lhe onlormalion submitted. 6ased en my ~ of lhe person or persons who manage lhe system. or those persons a.-eclly responsilla '"' gathenng lhe Sequoyah Site Vice President mfonnattcn. lhe trlormallon sobmtled is

  • to lhe bes1 or my knowledge and belief, ltUe. acarale. 423 843-7001 11 03 OS and complete I am aware 11\at lhere are signifocant penallies fDf subnuiling false onformalion. SIGNATURE OF PRINCIPAL EXECUTIVE I tneiUdwlg lhe poSStbilily of ftne and imprisonment for l<ncMvlg Y>Oiabons TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT
~I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS NATIONAL POULITANT DISCHARGE ELIMINATION SYSTEM (NPDES ) (Include Facility NameA..ocation if Dtfferent) MAJOR Form A PProved. DISCHARGE MONITORING REPORT (DMR) ~m~-N~SEOUOY~~UCLEARP~ --- (SUBR01 ) OMB No, 2040-0004 &Idress _p.O.JtOX 2000_ _ _ _ _ _ _ _ _ _ _ _ ---~INTEROFFICUB-~SQ~- -- - --- --- ~~ID~AI~TN23M_ _ _ _ _ _ _ _ ~-P-;_RN_M.~T;. o~  ;. . ;. 5. s;.0.'-ER-~II DlscHARGE1~~M~ER I ~~~~~~D coouNG wATER

                                                                                                                         . ;. . ;.64
                                                                                                                                  .uM

&11~ -~~EQ@~~~~RP~NC _ _ _ _ EFFLUENT ~~oJAMUO~OU~---------- MO 02

                                                                                                                                                                                            .... NO DISCHARGE           IXX I *""*

AnN: stephanie A. Howard NOTE: Read instructions before completifl!llhis form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREDUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** MEASUREMENT 04 04 CENTIGRADE 00010 z 0 0 PERMIT

                                                                           ********                    ********                 DEGC                      ********           .........**             38.3             DEGC               DAILY          GRAB4 REQUIREMENT INSTREAM MONITORING                                                                                                                                                                               DAILYMX PH                                               SAMPLE MEASUREMENT
                                                                            ********                   *'* ******                      ..                                    ............                               12 00400     1    0 EFFLUENT GROSS VALUE 0                             PERMIT REQUIREMENT
                                                                            "'*******                  ********                      -                      6.0 MINIMUM
                                                                                                                                                                             ...........              9.0 MAXIMUM su              WEEKLY           GRAB SOLIDS, TOTAL SUSPENDED                            SAMPLE MEASUREMENT
                                                                                                                                       -                  ********           ***'*****                                  19 00530    1     0 EFFLUENT GROSS VALUE 0                             PERMIT REQUIREMENT
                                                                            ********                   ********                      -                    *"***"**           ********                  30 DAILYMX MG/l               DAILY        COMPOS OIL AND GREASE                                    SAMPLE MEASUREMENT
                                                                                                                                        -                 ********            ********                                  19 00556    1     0 EFFLUENT GROSS VALUE 0                             PERMIT REQUIREMENT
                                                                            **"*****                   ****"****                     -                    ********            *******"*                15 DAILYMX MG/l               DAILY          GRAB FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT                                                                              03                 ********            ********              ***"*****            ..

PERMIT MGD *****"***

                                                                                                                                                                                                                        ...,.             DAILY         CALCTO 50050     1    0     0                                                     REPORT                     REPORT                                              *******"*           ********

REQUIREMENT EFFLUENT GROSS VALUE MOAVG DAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE *******"* ******** MEASUREMENT 19 50060 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT

                                                                             ...........                *****"***                     -                   "*****"***          ********                0.10 DAILYMX MG/l             WEEKLY          GRAB-4 SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certtfy under penally or law thal this dowment and all aUadvnenls were prepared under my                                                                                    TELEPHONE                     DATE
                                                     '"'coon or supervtsiOO in accordance with a system designed to assure that qualified person-lei Michael D. Skaggs                            rty gathet and ev~e lhe rrlormatioo SllbrniHed. Based m my nq'-*y of the person or s ..no manage the system. 0< those persons diredly responsible for gathering the                            Sequoyah it: $           resident ni01T11alion. the IAfonnalion stAmitted is , lo the best of my knowledge and belief, true, aocuate, 1---- - - - - - - - = = - - - - - ----4                   423         843-7001        11       03    05 I Sequoyah Site Vice President                      comple te. 1a m aware that there are signlfocanl penalties fa< sU>rrnlling false inlormaltOn,               SIGNATURE OF PRINCIPAL EXECUTIVE f -- - - - - - - - - - - - - - - l i n c l u d i n g the posstbil~y affine and impOsonment IO< knowing violalions.

OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (ReFerence all attachments h ere No Discharge this Period EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (lncJude Facility NameA.ocation ff Different) NATIONAL POLLUTANT DISCHARGE EUMINATION SYSTEM (NPDES) MAJOR Form A pproved_ .N_ame_ _TVA_:_ SEOUOYAH~ UCLEAR P~ - _ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Mdr~_p~_JtOX~- --- - - - _ - - - - --- ~INTER~~E SB-~-sa~ - - - --- - --- ~WM~M~- W ~~- -- -- -- - Fadi~ _NA~~YA~~~AR P~m_ _ _ _ _ Lo~tio~AMI~OU~- - - -- - - --- I From x;~R I ~~ r : vTr iNG e;~i 01 To ~~:1:1:::0:2::::2:8~ MO DAY EFFLUENT

                                                                                                                                                                             ... NO DISCHARGE          IXX I ..._

ATTN: stephanie A. Howard NOTE: Read instructions before completing lhis form_ X PARAMETER QUANTITY OR LOADING OUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC2 5 ST AT RE 7DAY CHR SAMPLE ******** ******** ... ******** ******** 23 CERIODAPHNIA

~RP3B     1     0    0 MEASUREMENT PERMIT REQUIREMENT
                                                                                                                        .....               45.2                ........ *         ........        PERCENT                SEMI       COMPOS EFFLUENT G ROSS VALUE IC25 S TAT RE 7DAY CHR                      SAMPLE MEASUREMENT MINIMUM
                                                                                                                                                                ********           ....... . .         23 A NNUAL PIMEPHALES ITRP6C     1    0 EFFLUENT GROSS VALUE 0                       PERMIT REQUIREMEm
                                                                      ********                   .....***.              *-                  45.2 MINIMUM
                                                                                                                                                                ********           ********        PERCENT                SEMI A NNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER     1 Ce<tify under penalty of law that this docoolent and all allactvnenls we<e prepared under my                                                           TE~PH ON E                   DATE diredion or supervision in accordance with a system designed lo assure thai quaflfied petsonnel Michael D. Sk aggs             property gather and evaluate the onfonnation SLtlm!tted. Based on my onquiry ollhe petson or persons who manage ll1e system, or those persons directly responsi>le for IJalhering the Sequoyah Si: '6.DPresident Sequoyah Site Vice President irtonnation, ll1e onlonnatial sobmilted is
  • co the best ol my knowledge and belief. true. accurate. 423 843-7001 11 03 05 and canplete. I am aware thallhere are signifiCatll penalties for sli>mitting false information. 1--- - -- -- -- - - - - - ---l SIGNATURE OF PRINCIPAL EXECUTIVE l -- - -- - - - -- - - - - ---1ncluding ll1e possobitity of fine and n.>risorvnent for knowing viOlations. OFFICER OR AUTHORIZED AGENT NUMBER TYPED OR PRINTED YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS £Referen ce all attJchments her e No Discharge this Period EPA Form 3 320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/location ff Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESJ MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) NamL _W~SEOUOY~~UC~AAP~ --- (SUBR01) OMB No. 204~004 Mdress_p....Q.J!..OX 2.2Q(L _ _ _ _ _ _ _ _ _ _ _ t---P-;-R-~-~r-0-~64 -u-M5-s0_E_R_-ijj DlscHARc: ~~M~ER I F - FINAL ---~ INTER~FICUB-~*SO~ -- -- --- --- ~ODID~A~rni~- ------- BACKWASH Fadli~ _NA~EOUOYAHN~LEAR~NC ___ _

                                                                                                                                                       ~::i EFFLUENT

~~tio ~AMI~NCOU~---------- I t : ! T riNG ..... NO DISCHARGE D ~* ATIN: stephanie A. Howard NOTE: Read instructions before romplelinl:l this fomt. PARAMETER DEBRIS, FLOATING (SEVERITY) 01345 1 0 0

                                              ><   SAMPLE MEASUREMENT PERMIT AVERAGE OUANTITY OR LOADING MAXIMUM UNITS MINIMUM OUALITY OR CONCENTRATION AVERAGE MAXIMUM REPORT 0

UNITS 9A PASS=O NO. FREQUENCY EX 0 OF ANAlYSIS 1 I 28 SEE SAMPLE TYPE VISUAL VISUAL REQUIREMENT FAll=1 EFFLUENT GROSS VALUE MOTOTAL PERMIT OIL AND GREASE VISUAL SAMPLE ******** 0 94 ******** '******'** ............. ** 0 1 I 28 VISUAL MEASUREMENT 84066 1 0 0 PERMIT ****'**** REPORT YES=1 ******** ******** ********

                                                                                                                                                                                                                  ....                 SEE          VISUAL REQUIREMENT                                                                 NO:O EFFLUENT GROSS VALUE                                                                                MOTOTAL                                                                                                                         PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEITmE PRINCIPAL EXECUTIVE OFFICER               I Cerufy under penalty of law lhal this doaJmenl and a ll attachments were prepared Wlder my                                                                 TELEPHONE                     DATE direc:lion or superv~soon in accordance With a system designed to assu-e thai qualifoed personnel Michael D . Skaggs                              gather  and eva!ua\e  the onfonnalion submitted. Based on my ~        of the person  or who m;nage the syslem. ex those persons <Wectly responsible for gathemg the informabOn. the onforma6on S<bnltled is
  • ID the best of my knowledge and belief, lnJe. accuate, Sequoyah Sitw : esident 423 843-7001 11 03 05 Sequoyah Site Vice PresiOent and complete. I am aware ltlal there are signofocanl penalties lex submilling false infonnalion. 1----:--:-:--:-::-----:-::-::-::-::----:------:-----i SIGNATURE OF PRINCIPAL EXECUTIVE 1 - - - - - - - - - - - - - - - - - - ; i n d u d i n g the possibilay or f111e and ""risorvnent lex knowing VJOiations OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS fReference all attachments here Operations performs visual inspections for floating debris and oil and grease during all backwashes.

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPD£5) PERMITTEE NAME/ADDRESS (Include FaCifitv Name/location 1f DifferenO MAJOR Form ApProved. DISCHARGE MONITORING REPORT (DMR) @m~-N~SEOU~A~UC~ARP~~ --- (SUBR 01) OMB No. 2040-0004 Adg[e~_p..Q.JtOX.~ -- _________ _ _ _ _ _ UNJERQEI3C~fl:M*SWL _ _ _ _ _ - - ---~~DY~AI~TNl~ ------- FaQ!lt'L_ _ TVA..:_SEQUOYAii!-.IUC!....E_AR PLANT_ _ _ _ _ LO~~ -~MIU~~~@_ ________ _ EFFLUENT ATTN: stephanie A. Howard QAY 28

                                                                                                                                                                                                                                        -  NO DISCHARGE          D -

NOTE: Read instructions before complelill!llhis loon.

                                                                                         ><                                                                                                                                                                               NO. FREQUENCY SAMPLE PARAMETER                                                                                                           QUANTITY OR LOADING                                                    QUALITY OR CONCENTRATION E.X        OF           TYPE ANALYSIS DEBRIS, FLOATING (SEVERITY)                                                                           SAMPLE MEASUREMENT AVERAGE MAXIMUM                 UNITS MINIMUM AVERAGE MAXIMUM 0

UNITS 9A 0 1/28 VISUAL PERMIT ******** "*'**"*** ****11:*** PASS=O SEE 01345 1 0 0 REQUIREMENT

                                                                                                                                 ********                                                                                                  REPORT                                                VISUAL FAIL=1 EFFLUENT GROSS VALUE OIL AND GREASE VISUAL                                                                                SAMPLE MEASUREMENT
                                                                                                                                 ********                        0                   94 MOTOTAL
                                                                                                                                                                                                                                            ********             ..        0 PERMIT 1 / 28        VISUAL 84066                 1         0            0                                                        PERMIT REQUIREMENT
                                                                                                                                 ********               REPORT                   YES=1 NO=O
                                                                                                                                                                                                      ********            ********          .........           ....                SEE          VISUAL EFFLUENT GROSS VALUE                                                                                                                                   MOTOTAL                                                                                                                   PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert.\fy oodet penalty of law thai this <lo<:oolent and all atladwnents were prepared o.nlet my
                                                                                                                                                                                                                                 ~resident TELEPHONE                     DATE i---=-::.::.=..:..:.:-==..:....:.:.::..:..::::..:c.:..=..=.:::.=:...:..:...c=....=..:....:...:..::.=:..-id.r.eciJOn or supernsoon on acoordance With a system designed lo assure that qualified personnel Michael D. Skaggs                                                          propel1y ga1her and evaluate the riormalion Slbnitted Based on my onqwry of !he person or persons who manage lhe system. or those persons directly responsible for galhering lhe Sequoyah Sequoyah Site Vice President ll"lformanon, the nonnation submitted is
  • to the best of my knowledge and behef. true. aca.ra~e. 1-- - - - - - - - - - - - - - - l 423 843-7001 11 03 05 complete I am aware that !here are sognifocant. penalties for submlllong false onformaliorl. SIGNATURE OF PRINCIPAL EXECUTIVE r-----~-=--=-::-:-==-=-----ioncluding !he possibility orr.,., and mprisoMienl for Mowing VIOlations OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here Operations performs visual inspections for floating debris and oil and grease during all backwashes.

EPA Fonn 3320-1 (REV 3/99) Prewous editiOns may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Faa1itv NameA.ocation if D1Herent} NATlONAI. POUliTANT DISCHARGE EUMtNATlON SYSTEM (NPDES} MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMRJ Nam~ -~~SEOUOYA~UC~ARP~ --- (SUBR 01) OMB No. 2040-0004 Address __P...Q._!!_OX 200Q_ _ _ _ _ _ _ _ _ _ _ _ 0264 5 0 ---~IN~R~IC~~~-S~L------- --- ~ODID~AI~lli~-------- F~~ -~~ EOUOYAHN UC~ARP~NL _ _ _ _ t--P-:-RN_M-~T--N -s-ER---ill

                                                                                                                             -u -M                o1scHARa;            ~~M~ER I :~~ATER                              & s To RM w ATE R Lo~o    ~ AMIN CO U Nn        _________ _

r:~T9RING 1-'" p~..... Iv;~R I ~~ [ 01

                                                                                                                                                           ;g "+:_...M.w...

O +-D """'p.y'"'""l EFFLUENT From ] TO 1.__;1_1_}...__0'-2___..---"- 2-'- 8__, ..... NO DISCHARGE lxx I ..... ATIN: stephanie A. How ard NOTE: Read instructions before completinq lhis form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF nPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS O XYGEN , DISSOLVED (DO) SAMPLE ******** MEASUREMENT 19 00300 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT

                                                                         ********                  ********                    -                 2.0 DAILYMN
                                                                                                                                                                            ********                     ..... . ..          MGIL             TWICE/

WEEK GRAB SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT

                                                                         *"*******                 ********                      ...         **'****"**                     ********                                          19 00530     1        0    0                       PERMIT REQUIREMENT
                                                                         ********                  ..........*                 -             .........                      . . ......                      100              MGJL             TWICE/         GRAB EFFLU ENT GROSS VALUE                                                                                                                                                                                   DAILY MX                                WEEK SOLIDS, SETTLEABLE                              SAMPLE MEASUREMENT
                                                                         ********                  ********                      ...         ********                        **'*"*****                                       25 00545      1       0    0                       PERMIT                    .........                 ********
                                                                                                                               ....          ********                        ********                       1.0              Mlll              O NCE/        G RAB REQUIREMENT EFFLUENT GROSS VAL UE                                                                                                                                                                                    DAILYMX                                MONTH FLOW, IN CONDUIT OR THRU                       SAMPLE MEASUREMENT                                                                           03           **'******                      ********                     ******"""*          ..

jTREATMENT PLANT 50050 1 0 0 PERMIT REQUIREMENT REPORT REPORT MGD ................ ***"*****

                                                                                                                                                                                                                               .               ONCE/         ESTlMA EFFLUENT GROSS VALUE                                                   MOAVG                     DAILY MX                                                                                                                                      BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT I
                                                                                                                                                                 ~,::!::.W:~~om NAME/TITlE PRINCIPAL EXECUTIVE OFFICER        I Certify U'lder penalty of law that lhos docunenl and all allachnenls were prepared ooder my                                                                                TELEPHONE                    DA~
                                                ~ edion or supe<vislon Vl     accordance Wllh a system designed to assure that quafofied persomel M ichael D. Skaggs               ""operty gather and evatuale lhe llllormallon submitted. Based on my lllqUiry of lhe person or persons wf1o manage the system, or those persons directly responsible for galherWig lhe Seq uoyah Site Vice President irlormaiJon. lhe information StJbmitted is
  • to lhe best or my knowledge and befoef, ttue, acx:uale, 423 843-7001 11 03 05 and oomplele I am ;rware that there are sognilicant penalties f or subrnillJtlg false irtormabon, SIGNATURE OF PRINCIPAL EXECUTIVE I
I oncluding lhe possibility of fine and impnsonment for known>g VIOlations.

OFFICER OR AUTtJORIZED AGENT NUMBER YEAR MO DAY n PED OR PRINTED COMMENTS AND EXP~NATION OF ANY VIO~TIONS fReference all attachments here During this reporting period. there has been no flow f rom the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- February 2011 DMR ORGANIZATION: Environmental DOCUMENTPREPAREDBY: BrndLove DATE: 03/5/2011 CONCURRENCES Name R c Signature - Comment Date v N B.M. Love X ...g_ 7~ ~ {oS/ZO t\

                                       '<._.../

S. A. Howard X v~hcuu.L ('( .<t:Lc~.-o (<--{ c ~~/S/1 I D.A. Day X (fJ. ()Q ~/ 3/7 j11 M.D. Skaggs X r\4t;~ u'  ?/1/11 U() INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with comm itments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction , and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 110412 800- NPDES CORRESPONDENCE April12, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR MARCH 2011 Enclosed is the March 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 3/19/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supeNision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, (l~ Michael D. Sk]25 Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 B. E. Brickhouse, LP 5U-C D. B. Nida, LP 5U-C G. M. Cook, OPS 4A-SQN A. A. Ray, WT 11A-K D. A Day, POB 2A-SQN G. R. Signer, WT 6A-K S. A Howard , OPS 5N-SQN M. D. Skaggs, OPS 4A-SQN K. Langdon, POB 2B-SQN K. M. Hodges (EDMS), LP 2V-C

Tennessee Valley Authority, Post Office Box 2000. Soddy Daisy, Tennessee 37384-2000 April12, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR MARCH 2011 Enclosed is the March 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 3/19/20 11 have all yielded resu lts below detection limits for oil and grease. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, ~.~o?!;lZ) Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCall ie Avenue Chattanooga, Tennessee 37402-2013

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different) MAJOR Fonn Approved. DISCHARGE MONITORING REPORT (DMR} Nam~-~~SEQU~A~UCLEARP~~--- (SUBR 01) OMB No. 2040-0004 Address_p...Q.JtOX 2QOO_ _ _ _ _ _ _ _ _ _ _ _ ---~MEROFFICESB-~-S~L ---~~~AISY.TN37~-------- f=-=.,=:;.;R~.;,;o;.;I~;.;:~: .: :~;.:5.Ms~0~e=R--=lll mscHARo:~~M~eR I ~~~~~:R DisCHARGE Faciii~-~~EQUO~HNUCLEARP~NL ___ _ Locatlo~AMIOONC~N~---------- I f%~TiRING pi:lgt MO DAY EFFLUENT

                                                                                                                                                                                        ***  NO DISCHARGE          D ...

ATTN: stephanie A. Howard NOTE* Read instructions before completing this form [X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF ~PE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS SAMPLE ******** ******** ******** ******** 28.6 31/31 RCORDR TEMPERATURE, WATER DEG. MEASUREMENT ** 04 0 CENTIGRADE 00010 1 0 PERMIT ******** ******** **** ******** ******** Req. Mon. DEG.C. CONTI CALCTD REQUIREMENT EFFLUENT GROSS DAILY MAX NUOUS SAMPLE ******** ******** ******** ******** 14.0 31/31 MODELD TEMPERATURE, WATER DEG. MEASUREMENT ** 04 0 CENTIGRADE 00010 z 0 PERMIT REQUIREMENT

                                                                           ********                  ********                          ****              ********           ********             30.5           DEG.C.              CONTI        CALCTD INSTREAM MONITORING                                                                                                                                                                         DAILVMX                                 NUOUS TEMP. DIFF. BETWEEN SAMP. &                      SAMPLE                    ********                  ********                                            ********           ********               1                         0      31/31        CALCTD MEASUREMENT                                                                                 **                                                                        04 UPSTRM DEG.C 00016     1    1                                 PERMIT                    ********                  ********                          ****              ********           ********               5            DEG.C.               CONTI       CALCTD REQUIREMENT EFFLUENT GROSS                                                                                                                                                                               DAILVMX                                NUOUS FLOW, IN CONDUIT OR THRU                         SAMPLE                    ********                     1642                                             ********           ********           ********                      0      31/31       RCORDR MEASUREMENT                                                                                03                                                                          **

TREATMENT PLANT 50050 1 0 PERMIT REQUIREMENT

                                                                           ********                Req. Mon.                          MGD                ********           ********          ********             ....              CONTI      RCORDR EFFLUENT GROSS                                                                                   DAILY MAX                                                                                                                          NUOUS CHLORINE, TOTAL RESIDUAL                        SAMPLE                    ********                   ********                                           ********             0.011             0.024                        0      16/31         GRAB MEASUREMENT                                                                                 **                                                                        19 50060     1    0                                 PERMIT                   ********                   ********                          ****             ********              0.1                0.1             MG/L            FIVE PER CALCTD REQUIREMENT EFFLUENT GROSS                                                                                                                                                             MOAVC           DAILY MAX                                WEEK TEMPERATURE- C, RATE OF                          SAMPLE                    ********                         0                                            ********           ********                                        0       31/31       CALCTD MEASUREMENT                                                                                62                                                                         **

CHANGE 82234 1 0 PERMIT ******** 2 DEG ******** ******** ******* .... CONTI CALCTD REQUIREMENT C/HR EFFLUENT GROSS DAILVMX NUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT

                                                                                                                                                                            ~

NAME/TinE PRINCIPAL EXECUTIVE OFFICER Michael D. Skaggs I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the r1 '7f L56 TELEPHONE DATE Sequoyah Site Vice President Sequoyah Site Vice President information. the information submitted is

  • to the best of my knowledge and belief. true, accurate.

and complete. I am aware that there are significant penalties for submitting false information. 423 I 843-7001 11 04 12 SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment tor knowing violations.

              ~PED OR PRINTED OFFICER OR AUTHORIZED AGENT
                                                                                                                                                                                                            ~~~~I       NUMBER        YEAR      MO   DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS £Reference all attachments here No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: Biodetergent 73551 (max. calc. cone. was 0.02mg/L-Iimit 2.0mg/L)

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp. ("C) Temp.("C) LOCATION Clams/m3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-ISV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB 02/08/2011 0 0 9 02/08/2011 0 9 1-25-545 MJW 02/15/2011 0 0 23 02/15/2011 0 23 1-25-545 MLW 02/22/2011 20 100 10 02/22/2011 0 10 1-25-545 PB 03/01/2011 0 0 11 03/01/2011 0 11 1-ISV-24-1236 PB 03/08/2011 0 0 11 03/08/2011 0 11 1-ISV-24-1236 WE 03/16/2011 22 0 11 03/16/2011 0 11 1-ISV-24-1234 MLW 03/23/2011 0 0 11 03/23/2011 0 11 1-ISV-24-1234 MLW 03/30/2011 0 0 12 03/30/2011 0 12 1-15v-24-1236 MLW

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) PERMITTEE NAME/ADDRESS (Include FacilitY Name/Location if Different) MAJOR Fonn Approved. DISCHARGE MONITORING REPORT (DMR) Name TVA....:_ SEQUOYAH~UCLEAR PLAN!_ _ _ _ (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000_ _ _ _ _ _ _ _ _ _ _ _

        ~INTEROFFICESB-2A*S~L _ _ _ _ _ _ _

===~~~AI~TN~3~------- Facllltv _TVA~EQUOYAH NgLEAR.PLANT_ _ _ _ _ EFFLUENT ~catlo~A~NNCN~----------

                                                                                                                                                                        *** NO DISCHARGE           D      ***

ATTN: stephanie A. Howard NOTE: Read instructions before completing this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRAnON NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ******** ******** ** Monitoring ******** ******** 23 MEASUREMENT CERJODAPHNIA Not Required TRP3B 1 0 PERMIT ******** ******** **** 43.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MINIMUM ANNUAL JC25 STATRE 7DAY CHR SAMPLE ******** ******** ** Monitoring ******** ******** 23 MEASUREMENT PIMEPHALES Not Required TRP6C 1 0 PERMIT ******** ******** **** 43.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT _L_ W.~v.:~nt NAME/TinE PRINCIPAL EXECUTIVE OFFICER 1Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 11 04 12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE I

                 ~PED  OR PRINTED OFFICER OR AUTHORIZED AGENT
                                                                                                                                                                                            ~~~~I       NUMBER        YEAR      MO   DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS             (Reference all attachments here Toxicity was not sampled in March 2011.

EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} MAJOR Form Approved. Name TVA_:_ SEOUOYAH~UCLEAR PLANT _ _ _ DISCHARGE MONITORING REPORT (DMR} (SUBR 01) OMB No. 2040-0004 Address P...Q.BOX2Q!)O_ _ _ _ _ _ _ _ _ _ _ _ ---~INTEROF~ESB-~-~~------- ___ jOD~~~~rn~~L ______ _ Facllltv _TVA..:_SEQ!JQYAH NUCLEAR PLANT_ _ _ _ _ ~c~o~AMI~~OUNTI_ _ _ _ _ _ _ _ _ _ EFFLUENT ATTN: stephanie A. Howard

                                                                                                                                                                       ***  NO DISCHARGE          D      ***

I>< NOTE: Read instructions before completing this form PARAMETER QUANTrrY OR LOADING QUALrrY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH 1 0 SAMPLE MEASUREMENT PERMIT 7 6 8 12 su 0 15/31 GRAB 00400 REQUIREMENT 9 THREE/ GRAB EFFLUENT GROSS MINIMUM MAXIMUM WEEK SAMPLE ******** ******** ******** 14 SOLIDS, TOTAL SUSPENDED MEASUREMENT ** 16 19 0 2131 GRAB 00530 1 0 PERMIT ******** ******** ** ******** 30 100 MGIL lWICEI GRAB REQUIREMENT EFFLUENT GROSS MOAVO DAILYMX MONTH SAMPLE ******** ******** <5 OIL AND GREASE MEASUREMENT

                                                                      ********                                           **                                                      <6               19        0       2131         GRAB 00556      1    0                            PERMIT                   ********                   ********                **              ********              15                20              MGIL             lWICEI         GRAB REQUIREMENT EFFLUENT GROSS                                                                                                                                             MOAVO           DAILYMX                                MONTH SAMPLE                                               1.089                                   ********          ********

FLOW, IN CONDUIT OR THRU MEASUREMENT 0.964 03 ******** ** 0 31/31 RCORDR TREATMENT PLANT 50050 1 0 PERMIT REQUIREMENT Req. Mon. Req. Mon MGD ******** ******** ********* .. SEE RCORDR EFFLUENT GROSS MOAVO DAILYMX PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT (l,.'i*~~ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 11 04 12 and complete. I am aware that there are significant penalties for submitting false information, including the possibility of line and imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE I

                                                                                                                                                                                           ~~~~I OFFICER OR AUTHORIZED AGENT                     NUMBER         YEAR      MO    DAY TIPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS            (Reference all attachments here EPA Form 3320-1 (REV 3/99)        Previous editions may be used                                                                                                                                               Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facilitv NameA..ocation if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM {NPDES} MAJOR Fonn Approved. Name_ _TVA_:_ SEOUOYAH~UCLEAR PLAN!_ _ _ _ DISCHARGE MONITORING REPORT {DMR} (SUBR 01) OMB No. 2040-0004 AddressJ..Q.JtOX 2000_ _ _ _ _ _ _ _ _ _ _ _ ---~INTEROFFICUB*2A*SQ~------- ---~OD~~M~rn37~------- Facllltv _TVA..:_SEQUOYAH N.!,K:LEAR.PLANT_ _ _ _ _ EFFLUENT ~catlo~AMIOO~N~---------- MQ 03

                                                                                                                                                                         *** NO DISCHARGE           IXX I ***

ATTN: stephanie A. Howard NOTE: Read instructions before completing this fonn PARAMETER TEMPERATURE, WATER DEG.

                                        ><   SAMPLE MEASUREMENT AVERAGE QUANTITY OR LOADING MAXIMUM UNITS MINIMUM QUALITY OR CONCENTRATION AVERAGE MAXIMUM            UNITS 04 NO.

EX FREQUENCY OF ANALYSIS SAMPLE TYPE CENTIGRADE 00010 1 0 PERMIT REQUIREMENT

                                                                        ********                  ********                 -               ********           ********        REPORT              DEGC              CONTIN uous CALCTD
                                                                                                                           --                                                DAILYMX EFFLUENT GROSS VALUE TEMPERATURE, WATER DEG.                      SAMPLE                     ********                  ********                                 ********           ********

MEASUREMENT 04 CENTIGRADE 00010 z 0 PERMIT ******** ******** ******** ******** 30.5 DEGC CONTIN CALCTD REQUIREMENT DAILYMX uous INSTREAM MONITORING TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** 04 MEASUREMENT UPSTRM DEG.C 00016 1 0 EFFLUENT GROSS VALUE PERMIT REQUIREMENT

                                                                        ********                  ********                  -              ********           ********             5 DAILYMX DEGC              CONTIN uous CALCTO FLOW, IN CONDUIT OR THRU                      SAMPLE                    ********                                                           ********           ********         ********

MEASUREMENT 03 ** TREATMENT PLANT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ******** ******** .. CONTIN RCORDR EFFLUENT GROSS VALUE REQUIREMENT DAILYMX uous CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** MEASUREMENT ** 19 50060 1 0 PERMIT ******** ******** ** ******** 0.1 0.1 MGIL Five per CALCTO REQUIREMENT EFFLUENT GROSS VALUE MOAVG DAILYMX Week TEMPERATURE- C, RATE OF SAMPLE ******** ******** ******** ******** 04 ** CHANGE 82234 1 0 MEASUREMENT PERMIT REQUIREMENT

                                                                         ********                       2               DEGC                ********          ********          ********             .               CONTIN       CALCTD EFFLUENT GROSS VALUE                                                                           DAILYMX                                                                                                               uous SAMPLE MEASUREMENT PERMIT REQUIREMENT I I Q~~~w.m NAME/TITLE PRINCIPAL EXECUTIVE OFFICER      I Certify under penalty of law that this document and all attachmenls were prepared under my                                                        TELEPHONE direction or supervision in accordance wilh a system designed to assure that qualified personnel DATE Michael D. Skaggs                property gather and evaluate the informalion submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate,                                           423       843-7001         11      04     12 and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violalions.

SIGNATURE OF PRINCIPAL EXECUTIVE I

                                                                                                                                                                                             ~~~~I OFFICER OR AUTHORIZED AGENT                     NUMBER         YEAR      MO    DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS              (Reference all attachments here No Discharge this Period.

EPA Form 3320*1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAl POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved. Name TVA_:_ SEOUOYAH~UCLEAR PLAN!_ _ _ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O.JiOX 200Q_ _ _ _ _ _ _ _ _ _ _ _

       ~INTEROFFICESB-~-S~L _ _ _ _ _ _ _

F- FINAL ---~OD~~AI~.TN~~L _ _ _ _ _ _ _ RECYCLED COOLING WATER Faciii~-~~EQU~AHNUCLE~P~NL _ _ _ _ r%~~TriNG e;:;gt EFFLUENT LOCMIO~A~WNCN~---------- I

                                                                                                                                              ~~:1:1~~:03=~~=31:~

MO From 11 YEAR I 03 MO 01 To DAY

                                                                                                                                                                         *** NO DISCHARGE           IXX I ***

A1TN: stephanie A. Howard NOTE: Read instructions before completing this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 0 SAMPLE MEASUREMENT PERMIT

                                                                                                                        ****              43.2 23 PERCENT                SEMI       COMPOS REQUIREMENT MINIMUM                                                                       ANNUAL EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR                       SAMPLE                     ********                 ********                                                      ********          ********

MEASUREMENT 23 PIMEPHALES TRP6C 1 0 0 PERMIT ******** ******** 43.2 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

                                                                                                                                                       .!~.'!~"'

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law !hat this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submilled. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submilled is , to the best of my knowledge and belief, true, accurate, 423 843-7001 11 04 12 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submilling false information, including the possibility of fine and imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE I

             ~PED   OR PRINTED OFFICER OR AUTHORIZED AGENT
                                                                                                                                                                                             ~~~~I       NUMBER        YEAR      MO    DAY COMMENTS AND    EXP~NATION  OF ANY VIO~TIONS         (Reference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3199)       Previous editions may be used                                                                                                                                                  Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} MAJOR Fonn Approved. Name TVA_:_ SEOUOYA~UCLEAR PLAN!_ _ _ _ DISCHARGE MONITORING REPORT (DMR} (SUBR 01) OMB No. 2040-0004 Address P.O....R.OX 2000_ _ _ _ _ _ _ _ _ _ _ _ ---~INTEROFFICESB-2A-SQ~------- ---~OD~~AI~.TN3~8L ______ _ Facilitv TVA..:.$EQUOYAH NUCLEAR PLANT_ _ _ _ _ EFFLUENT ~catio HAMI~NCOUN~----------

                                                                                                                                                                                      *"* NO DISCHARGE            IXX I **"

ATIN: Stephanie A. Howard NOTE: Read instructions before completing this form IX PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED 00300 1 0 (DO) SAMPLE MEASUREMENT PERMIT

                                                                                                                                 *"**                   2 19 MG/L              lWICE/         GRAB REQUIREMENT EFFLUENT GROSS                                                                                                                                   MINIMUM                                                                           WEEK SOLIDS, TOTAL SUSPENDED                               SAMPLE                    ********                   ********                                 ********              ********

MEASUREMENT ** 19 00530 1 0 PERMIT ******** ******** *"** ******** ******** 100 MG/L lWICEI GRAB

                                                  *REQUIREMENT EFFLUENT GROSS                                                                                                                                                                             DAILYMX                                 WEEK SOLIDS, SETILEABLE                                    SAMPLE                    ********                   ********                                 ********              ********

MEASUREMENT ** 25 00545 1 0 PERMIT ******** ******** **** ******** ******** 1 MUL ONCE/ GRAB REQUIREMENT EFFLUENT GROSS DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** MEASUREMENT 03 ** TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ******** ******** ********

  • ONCE! ESTIMA REQUIREMENT EFFLUENT GROSS MOAVG DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of Jaw that this document and all attachments were prepared under my TELEPHONE DATE Vi~nt direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 11 04 12 and complete. I am aware that there are significant penalties for submitting false information, 1--::::=-:-::-=:-=-=-::-::-::-:--::::-::-------l SIGNATURE OF PRINCIPAL EXECUTIVE f - - - - - - - - - - - - - - - - - l ' i n c l u d i n g the possibility of fine and imprisonment for knowing violations.
                 ~PED OR PRINTED OFFICER OR AUTHORIZED AGENT                         NUMBER       YEAR      MO     DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- March 2011 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 04/11/2011 CONCURRENCES Name R c Signature - Comment Date v N B.M. Love X S. A. Howard X D.A. Day X M.D. Skaggs X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 120201 802 - NPDES CORRESPONDENCE February 1, 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- CORRECTION TO DISCHARGE MONITORING REPORT FOR MARCH AND APRIL 2011. During the review of the Biocide/Corrosion Treatment Plan (B/CTP) Annual Report it was noted that there was an error reported for Frequency of Analysis in the March and April 2011 Discharge Monitoring Reports (DMR). Please see the attached corrected DMR pages. It should be noted that the chlorine, total residual maximum value and the chlorine, total residual average did not change as a result of the correction to the Frequency of Analysis for each month. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. rlin 1e jce President Seqtfoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 B. E. Brickhouse. LP 5U-C D. B. Nida, LP 5U-C J . T . Carlin, OPS 4A-SQN A. A. Ray, WT 11A-K G. M. Cook, OPS 4A-SQN G. R. Signer, WT 6A-K J . A. Cross, POB 2A-SQN P.R. Simmons, POB 2B-SQN S. W . Hixson, BR 4A-C B. N. Smith (EDMS), MPB 1E-M

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 February 1, 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT (SQN)- NPDES PERMIT NO. TN0026450- CORRECTION TO DISCHARGE MONITORING REPORT FOR MARCH AND APRIL 2011. During the review of the Biocide/Corrosion Treatment Plan (B/CTP) Annual Report it was noted that there was an error reported for Frequency of Analysis in the March and April 2011 Discharge Monitoring Reports (DMR). Please see the attached corrected DMR pages. It should be noted that the chlorine, total residual maximum value and the chlorine, total residual average did not change as a result of the correction to the Frequency of Analysis for each month. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~-~A-SEQUO~~UCL~RP~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Address_ ...f..O. BOX 20QQ.. _ _ _ _ _ _ _ _ .:..._ _ _ _ ---~TEROFFI~OPS-5N-S~-------- TN0026450 F- FINAL ---~D~-MISQ~73~---~---- PERMIT NUMBER DIFFUSER DISCHARGE Fa~-~A-SEQU~HNUCL~RP~NC _ _ _ _ _ EFFLUENT L~~~MI~NCO~~---------- ATTN: Brad Love

                                                                                                                                                                       ***  NO DISCHARGE          D***

NOTE: Read Instructions before completin!l this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ***'***** ******** ******** 28.6 31/31 RCORDR M~SUREMENT ** 04 0 CENTIGRADE 00010 1 0 PERMIT ******** ******** ******** ********* Req. Mon. DEG.C. CONTI CALCTD REQUIREMENT EFFLUENT GROSS DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 14.0 31/31 MODELD M~SUREMENT ** 04 0 CENTIGRADE 00010 z 0 PERMIT ******** ******** ******** ******** 30.5 DEG.C. CONTI CALCTO REQUIREMENT INSTREAM MONITORING DAILYMX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** 1 0 31/31 CALCTD M~SUREMENT ** 04 UPSTRM DEG.C 00016 1 1 PERMIT ******** ******** ******** ******** 5 DEG.C. CONTI CALCTD REQUIREMENT EFFLUENT GROSS DAILYMX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** 1642 ******** ******** ******** 0 31/31 RCORDR TR~TMENT P~NT M~SUREMENT 03 ** 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ******** ******** .... CONTI RCORDR REQUIREMENT EFFLUENT GROSS DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** 0.011 0.024 0 15/31 GRAB M~SUREMENT ** 19 50060 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT MOAVG 0.1 0.1 DAILY MAX MGIL FIVE PER CALCTD WEEK TEMPERATURE- C, RATE OF SAMPLE ******** 0 ******** ******** 31/31 CALCTD M~SUREMENT 62 ** 0 CHANGE 82234 1 0 PERMIT ******** 2 DEG ******** ******** ******* .... CONTI CALCTD REQUIREMENT C/HR EFFLUENT GROSS DAILYMX NUOUS SAMPLE M~SUREMENT PERMIT REQUIREMENT

                                                                                                                                                                /'
                                                                                                                                                  /     ,\- I .     /

I~~~;jJ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all aHachments ware prepared under my TELEPHONE DATE direction or supervision in accordance with a system designad to assure that qualifiad parsonnal John T. Carlin properly gather and evaluate tha information submlttad. Based on my inquiry of tha parson or persons who manega the system, or those persons directly responsible for gather1ng the resident 423 843-7001 information, the information submitted is , to the best of my knowledge and belief, true, accurate,

                                                                                                                                                           ~.,

12 01 17 Sequoyah Site Vice President S~ 0F PRINCIPAL EXECUTIVE 1 and complete. I am aware that there are significant penalties for submitting false Information, I TYPED OR PRINTED Including the possibility of fine and imprisonment for knowing violations. OFF R OR AUTHORIZED AGENT AREA CODE I NUMBER Y~R MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: Biodetergent 73551 (max. calc. cone. was 0.02mg/L-Iimit 2.0mg/L) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location ff Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na~-~A-S~UO~HNUCL~RP~NT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Addres.!_ ....f..Q,_BOX 2000 _ _ _ _ _ _ _ _ _ _ _ _ ---~TE~~EOPS-5N-S~-------- 101 G F- FINAl ---~D~-MI~~~73~-------- DIFFUSER DISCHARGE ~~-~A-S~O~HNUCL~RP~N~---- EFFLUENT ~cati~~~~NCOUN~---------- ATTN: Brad Love

                                                                                                                                                                              ***  NO DISCHARGE           o--

NOTE* Read instructions before completlna this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 30/30 RCORDR 34.4 0 M~SUREMENT ** 04 CENTIGRADE 00010 1 0 PERMIT ********* ******** ******** Req. Mon. DEG.C. CONTI CALCTD REQUIREMENT . EFFLUENT GROSS DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 19.7 30/30 MODELD M~SUREMENT ** 04 0 CENTIGRADE 00010 z INSTREAM MONITORING 0 PERMIT REQUIREMENT

                                                                              ********                   ********              -                 ********          ********           30~5 DAILYMX DEG.C.                     CONTI NUOUS CALCTO TEMP. DIFF. BETWEEN SAMP. &                          SAMPLE                    ********                   ********                                ********           ********            3                                0        30/30          CALCTD M~SUREMENT                                                                      **                                                                        04 UPSTRM DEG.C 00016        1      s                                PERMIT                   *********                   ********                                ********          ********             3               DEG.C.                    CONTI          CALCTO REQUIREMENT EFFLUENT GROSS                                                                                                                                                                     DAILYMX                                         NUOUS SAMPLE                                                1642                                    ********          ********                                                      30/30         RCORDR FLOW, IN CONDUIT OR THRU M~SUREMENT
                                                                              ********                                          03                                                  ********               **            0 TREATMENT PLANT 50050        1 EFFLUENT GROSS 0                                 PERMIT REQUIREMENT SAMPLE
                                                                              ********                Req. Mon.

DAILY MAX MGD ********

                                                                                                                                                  **1t*****
                                                                                                                                                                   ********         ********              -                       CONTI NUOUS RCORDR CHLORINE, TOTAL RESIDUAL                                                       ********                   ********               **                                   0.017           0.026                19             0        25/30           GRAB M~SUREMENT 50060        1 EFFLUENT GROSS 0                                 PERMIT REQUIREMENT
                                                                              ********                   ********              -                  ********            0.1 MOAVG 0.1 DAILY MAX MGIL                  FIVE PER CALCTD WEEK TEMPERATURE- C, RATE OF                              SAMPLE                    ********                        1                                   ********          ********                                                      30/30          CALCTO 62                                                                         **            0 CHANGE                                            M~SUREMENT 82234        1      0                                 PERMIT                   ********                        2               DEG                 ********          ********         *******                                      CONTI          CALCTD REQUIREMENT                                                                  C/HR EFFLUENT GROSS                                                                                         DAILYMX                                                                                                                     NUOUS SAMPLE M~SUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of Jaw that this document and all attachments were prepared under my                                                                    TELEPHONE                           DATE direction or supervision In accordance with a system designed to assure that qualified personnel John T. Carlin                   properly gather and evaluate the lnfonnalion submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the                                     President information, the information submitted is , to the bast of my knowledge and belief, true, accurate,                                           423         843-7001              12       01       17 Sequoyah Site Vice President                  and complete. 1am awsre that there are significant penalties for submitting false Information,       f-b---,l~-.,~--------.-.......1 1------:=-===--==-=====-------tincluding the possibility of fine and Imprisonment for knowing violations.                                                                                        r-:-AR=EA=-+--N""U""M:-=BE=-R=-.-tc-Y-EA-R-t-..,-M-O-f-D-A-Y-l TYPED OR PRINTED                                                                                                                                                                   CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No closed mode operation. Veliger Monitoring Data is includes as an attachment. The following injections occurred: 1. Towerbrom 960 2. Spectrus CT1300 (max. calc. cone. was 0.039mg/L-Iimit 0.050mg/L) EPA Fonn 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- March I April 2011 DMR Corrections ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 1119/2012 CONCURRENCES Name R c Signature - Comment Date v N B.M. Love X J.L. Grubb X J.A. Cross X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 110510 800- NPDES CORRESPONDENCE May 10,2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR APRIL 2011 Enclosed is the April 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond . Samples collected from 9/23/2010 through 4/30/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information , please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, ~ ~tas. Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington , DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 B. E. Brickhouse, LP 5U-C D. B. Nida, LP 5U-C G. M. Cook, OPS 4A-SQN A. A. Ray, WT 11A-K D. A. Day, POB 2A-SQN G. R. Signer, WT 6A-K S. A. Howard , OPS 5N-SQN M. D. Skaggs, OPS 4A-SQN K. Langdon, POB 2B-SQN K. M. Hodges (EOMS), LP 2V-C

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 May 10, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 61h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR APRIL 2011 Enclosed is the April 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection continues at the Diffuser Pond Inlet of the Yard Drainage Pond effluent because of a transformer oil spill that reached the Yard Drainage Pond. Samples collected from 9/23/2010 through 4/30/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Brad Love at (423) 843~6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supeNision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, ~..~~~ Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

PERMITTEE NAME/ADDRESS (Include Facilitv Namellocafion if Different) NATIONAL POllliTANT DISCHARGE EliMINATION SYSTEM (NPOES) MAJOR Fonn Approved. DISCHARGE MONITORING REPORT (DMR) Nam~ -N~SEOUOYA~UC~AAP~~ --- (SUBR 01) OMB No. 2040-0004 A..ddr~_P..Q..J!.PX ~- _ _ _ _ _ _ _ _ _ _ - --~INTER~~ESB -~*50~ ------- - --~@DY~AI~. m~~-------- Facili~ -~~~~YAHN~LEAR P~ID_ _ _ _ _ _____ _ ___ _ EFFLUE NT

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~c~o~A~~CNTI ATIN : stephanie A. Howard I I 04 [ YfAR MO 01 ] To l._-'- 1-'- 1 -']'---'- 04_;__J'---'- 30-'---' NO DISCHARGE D .... NOTE: Read instructions before complelin!l this form. X PARAMETER QUANTITY OR LOADING OUALITI OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS !TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 SAMPLE MEASUREMENT PERMIT

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NAME/TinE PRINCIPAL EXECUTIVE OFFICER Michael D . Skaggs I Certify under penally of law that this clocunent and all aUachments were prepared under my iredion or supetVision in a<X)()(dance with a system des~ lo assure that quaJffied persomel property gather and evakJate the lflforma!Jon Slbnotted Based on my inquiry ollhe person or persons who mMage the system, or those p<<SSnS directly responsible for gathenng the r-1 '-:>~ ~oz5 Sequoyah Site Vice President TELEPHONE DATE

                                              *rlormation, the informaloon S<bnitled is
  • to the best or my knowledge and belief, true. accuate. 423 843-7001 11 05 09 Sequoyah S ite Vice President and complete. I am awate lhal there are sigrl!f;cart penallies lor sltlm*lhng false information. _I SIGNATURE OF PRINCIPAL EXECUTIVE tnckJdlng the possililily of line and imprisonment for knowing violations.

OFfiCER OR AUTHORIZED AGENT AREA 1 NUMBER YEAR MD DAY TIPED OR PRINTED rnnF COMMENTS AND EXPLANATION OF ANY VIO~TIONS (Reference all attachments here No closed mode operation . Veliger Monitoring Data is includes as an attachment. The following injections occurred: 1. Towerbrom 960 2. Spectrus CT1300 (max.. calc. cone. was 0 .039mg/L-Iimit 0 .050mgfL) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp. ("C) Temp.("C) LOCATION Clams/m3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-JSV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB 02/08/2011 0 0 9 02/08/2011 0 9 1-25-545 MJW 02/15/2011 0 0 23 02/15/2011 0 23 1-25-545 MLW 02/22/2011 20 100 10 02/22/2011 0 10 1-25-545 PB 03/01/2011 0 0 11 03/01/2011 0 11 1-ISV-24-1236 PB 03/08/2011 0 0 11 03/08/2011 0 11 1-ISV-24-1236 WE 03/16/2011 22 0 11 03/16/2011 0 11 1-ISV-24-1234 MLW 03/23/2011 0 0 11 03/23/2011 0 11 1-ISV-24-1234 MLW 03/30/2011 0 0 12 03/30/2011 0 12 1-15v-24-1236 MLW 04/06/2011 18 100 15 04/06/2011 0 15 1-ISV-24-1234 HMW 04/08/2011 45 100 15.5 04/08/2011 0 15.5 1-1SV-24-1236 WAW/PB 04/20/2011 21 100 16 04/20/2011 0 16 1-1SV-24-1236 PB

PERMITTEE NAME/ADDRESS (Include FaCJ7itv Name/location if Different) NATIONAl POLLlJTANT DISCHARGE EliMINATION SYSTEM (NPDES) MAJOR Form Approved. _N!lm e_ _TVA~ SEOUOYA~UCLEAR PLAN!_ _ _ _ DISCHARGE MONITORING REPORT (DMR) (SUBR 01} OMB No. 2040-0004 Address_P...Q.JtOX 2000 __ _ ___ _____ _ ---~INnR~~UB -~-S~L _ _ _ _ _ _ _ --- ~ODDY~AI~ rn m~-- ------ Facili~-~~ EQ~YAHNUC~RP~N_ _ _ _ _ 1---P-:-R-~-~1-0-~6-u45.;..

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r%:T! RING EFFLUENT ATTN: ste phanie A . Howard From ] To lL NO DISCHARGE NOTE: Read instructions before completing this form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRAnON NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE MEASUREMENT

                                                                                                                          -            Monitoring                     ********             ********            23 CERIODAPHNIA T R P38    1 EFFLUENT GROSS 0                              PERMIT REQUIREMENT
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!TRP6C 1 0 PERMIT REQUIREMENT Not Required 43.2 ******** ******** PERCENT SEMI COMPOS EFFLUENT GROSS MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT A NAME/TlTLE PRINCIPAL EXECUTIVE OFFICER M ichael D. Skaggs I Certify under penalty of law lhal llu document and all attachments were prepared Lnder my direction Of StJpetVosion on aa:ordance woth a syslem designed to a ssure that quafofoed personnel properly gather and evaluate lhe information submitted. Based on my irq.~ory a !he person or persons who manage the syslem. or lllose persons cforedly responsible rex galhenng the r'l 7~-60 TELEPHONE DATE Sequoyah Site Vice President Sequoyah Site V ice President ncnnatoon. the onformabon Stbnitled .s . to the best of my knowledge and belief, true, accurate, 423 843-7001 11 05 09 and complete I am aware lhat there are sognilicanl penalties rex soomottw>g lalse informabon, SIGNATURE OF PRINCIPAL EXECUTIVE I oncluding the possibil~y ol fone and ompn.sonmenl lor knowong violations TYPED OR PRINnD OFFICER OR AUTHORIZED AGENT

                                                                                                                                                                                                          ~~~~ I     NUMBER        YEAR       MO   DAY COMMENTS AND EXPlANATION OF ANY VIO~TIONS                (Reference ill/ iittilchments here Toxicity was not sampled in April 2011 .

EPA Fonn 3320-1 (REV 3199) Previous editions may be used P age 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAl POLLUTANT DISCHARGE EUM INATION SYSTEM (NPDES) MAJOR Form ApProved. DISCHARGE MONITORI NG REPORT (DMRJ m~--W~SEOUOY~~UCLEARP~~ --- (SUBR01) OMS No. 204()-0004 _&ldr§S_P...Q.JiOX ~ _ _ _ _ _ _ _ _ _ _ _ - --~ IN~~~U~~-S~L ------- --- ~ODDY~AI~rn~~-------- Facili~-~~ EQ~~~~~RP~~ ---- Lo~~~AMUO~@NIT ATTN: stephanie A. Howard I t : !Tr iNG e;mt EFFLUENT

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                                                                                                                                                                                                              ..                SEE         RCORDR EFFLUENT GROSS                                                         MOAVG                   DAILYMX                                                                                                                        PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify IJI"ode< penally of law lhallln doc:unent and an attaclvnents were prepared IJI"ode< my Michael D. Skaggs Sequoyah Site Vice President direchon or supennsion 1n accordance with a syseem OOs;gned to assue lhat qualified persornel property gather and evaluate the infOfTilalion submllled. Based on my inq<.Wy of the per5IX1 or persons who manage the syseem, or lhose persons diredly responsible ror gau-ong the 1nforma1Jon. the lflformabon submitted IS. 10 the best of my knowledge and belief, IIUe, accurate, I(~~
                                                                                                                                                          .....    /'

ra Sequoyah Site Vice President 423 TELEPHONE 843-7001 11 DATE 05 09 jand complete. I am aware rhat there are sogri6canl penai!Jes lor stbmotbng false information, SIGNATURE OF PRINCIPAL EXECUTIVE I ITPED OR PRINTED includ1ng the possibility of flOe and impnsonment lor knowing violations OFFICER OR AUTHORIZED ACE NT AREA rnnF I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all Jttachments here EPA Form 3320*1 (REV 3/99) Previous ed"mons may be used Page 1 of 1

PERMmEE NAME/ADDRESS (Include Faa1rtv Name/location if Different] NATIONAL POLLUTAm DISCHARGE arMINATION SYSTEM (NPDES} MAJOR Fonn APProved. NamL_~~SEOUOYA~UCLEAAP~~ --- DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Mdress_p..Q.J!.OX~ _ _ _ _ _ _ _ _ _ _ _ ---~I~ROF~ESB-~*SQ~ ------- --- ~OODY~~Y. TN~-------- Facii~ -~~EQ~YAHN~~ARP~ID_ _ _ _ _ J---P-;-R~-~-~-~.~.;. -M5.;a. 0.;ER. .---111 DISCHARGE 1

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AITN: stephanie A. Howard NOTE: Read inslructions before completinQ this form X PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ****"**** ******** "*

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cert1fy under penally of law thai this document and all atlachmenls were prepared tnder my TE~PHONE DATE

                                              .rectoon or superviSIOn in accordance wilh a system designed to assu-e thai qual-.r.ed personnel Michael D. Skaggs               property galller and evaluate the Jnf"""' tian submitted. Based on my inquiry of the person or persons who manage the system. or IOO;e persans drectly responsible for galhe<ing the Sequoyah Site Vice President nr~ the nonnation submitted is. to the best of my knawfe<lge and behef. true. accurate.                                                              423        843-7001        11      05    09
                                            ~nd complete. I am aware thallhere are signJfJCant penalties for submiUJng false inlonnation.

mcluding the possib1h1y ol fine and Impn>onment for knowing violations SIGNATURE OF PRINCIPAL EXECUnVE I

~~ I TYPED OR PRINTED OFFICER OR AUTHORIZED ACENT NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments herE No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include FaalitV Name/location if Different) NATIONAL POLLliTANT DISCHARGE ELIM INATION SYSTEM (NPDES} MAJOR Form ApProved. DISCHARGE MONITORING REPORT (DMR} m~--~~SEQUOYA~UCLEARP~ --- (SUBR 01 ) OMB No. 204G-0004 Address_p....Q.Jl0X.2..QQO_ _ _ _ _ _ _ _ _ _ _ _ ---~@WOFFICU~*SO~ ------- --- ~O~~Al~llil~-------- ficili~ -~~EQ~~~~L~~MNC _ _ _ _ EFFLUENT ~~~~A~ro~N~--------- - MQ 04

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ATIN: stephanie A . Howard NOTE: Read instructions before complelin!llhis form. X PARAMETER QUANT~ OR LOADING QUA~ OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE ANALYSIS IC25 STATRE 7DAY CHR SAMPLE MEASUREMENT *****-*** MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 23 CERIODAPHNIA TRP38 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT

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SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT (t;.~,:~L"""' NAME/TlTLE PRINCIPAL EXECUTIVE OFFICER I Cer1Jfy LRier penalty of law that U.S documeol and aD attachments were prepared o.n1er my TELEPHONE DATE uorec:IJon or supeMSJOn '" acccrdance With a system designed to ass.re thai qualified petsorne1 M ichael D . Skaggs propelly gather and evalua:e the infonnallon SUbmitted Basad 00 my inquiry or lhe person or persons wt1o manage the system, or those persons diredly responsible lor ga1herwlg the information. the information subnitled os

  • to the best or my knowledge and belief. true, acaJrate, 423 843-7001 11 05 09 Sequoyah S ite V ice P resident and complete I am aware that lhere ace signtrocanl penaltJes lor slbmitl.ng false information, SIGNATURE OF PRINCIPAL EXECUTIVE I
              ~PED      OR PRINTED onc:lu<Mg the possibility of fone and ~sorcnenl lor knDwong 1110labons OFFICER OR AUTHORIZED AGENT             AREA r-. nnF I   NUMBER       YEAR       MO   DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS                   fReference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3199)             Previous editions may be used                                                                                                                                                   Page 1 of 1

PERMITIEE NAME/ADDRESS NATIONAl POLLUTANT DISCHARGE ELIMINATIO N SYSTEM (NPDES) Form Approved. (Include Facility Name/Location if Differenl) MAJOR DISCHARGE MONITORING REPORT (DMR) ~m~ -~~SEOUOYAA~UC~ARP~~ --- (SUBR 01) OMB No. 2040-0004 Addr~_p..Q...§..OX 2..Q9Q_ _ _ _ _ _ ___ _ _ _ - - - ~ ~EROFFIC~B*2A*SQ~ ---- -- - - -- ~OD~~AI~lli~~-- -- -- - - Fa@~ -~~EQU~A~UCLEARP~NL _ _ _ _ EFFLUENT Lo~tio~~IUO~OUN~----- - - -- - ATTN: stephanie A. Howard From : Y~~R ~~ I 01 To :1:1:1:.+_. . """.:. ~"~-...'-_:-_...~:. . ;A"....t: NO DISCHARGE IXX I ~ NOTE: Read instructions before comPielill!llhis form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE ******** ******** ... ******** ................ 19 MEASUREMENT 00300 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT

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MEASUREMENT 25 00545 1 0 PERMIT REQUIREMENT

                                                                             ********                   ********                 **-               ********                          *******'*                            1            ML/l              ONCE/           GRAB EFFLUENT GROSS FLOW, IN CONDUIT OR THRU                           SAMPLE MEASUREMENT                                                                         03 DAILYMX MONTH TREATMENT PLANT 50050      1    0                                  PERMIT REQUIREMENT Req. Mon.                  Req. Mon.                  MGD               ********                           *****....*                    ********
                                                                                                                                                                                                                                          .              ONCE/          ESTlMA EFFLUENT GROSS                                                               MOAVG                     DAILY MX                                                                                                                                           BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEITlTLE PRINCIPAL EXECUTIVE OFFICER I Certify ooder penally of law that this doc:umenl ani al attactments we<e prepared tn1er my d ~ ~sident                           '

TELEPHONE DATE direc:bon << supeiVlSOOO W1 accordance with a system designed to assure thai quaflfied personrel M ichael D. Skaggs property gather and evaluate the information submitted. Based on my inQwy of the person << persons who manage the system, or those persons diredly responsible lor gathering the q oyah Site inlormatJoro, the 111l onnatioo submitted is , to the best of my """'"edge and beltef, true, acx:urate. 423 843-7001 11 05 09 Sequoyah Site Vice President and complete I am aware that there are signtficanl penalbes for sl.brrotbng false inlormallorl. SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility or fone and mprisonment for knowing violations TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT

                                                                                                                                                                                                                                    ~~~~ I   NUMBER         YEAR      MO    DAY COMMENTS AND EXP~NATION OF ANY VIOLATIONS (Reference all attachments here During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

EPA Form 3320-1 (REV 3199) Previous efii bons may be usefi Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- April2011 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 05/09/2011 CONCURRENCES Name R c Signature - Comment Date v N B.M. Love X S. A. Howard X D.A. Day X M.D. Skaggs X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 110608 800 - NPDES CORRESPONDENCE June 8, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 1 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR MAY 2011 Enclosed is the May 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection at the Diffuser Pond Inlet of the Yard Pond effluent due to a transformer oil spill that reached the Yard Drainage Pond has been discontinued as of 5/14/2011. Samples collected from 9/23/2010 through 5/14/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supe!Vision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. d~?t~ Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 B. E. Brickhouse, LP 5U-C D. B. Nida, LP SU-C G. M. Cook, OPS 4A-SQN A A Ray, WT 11A-K D. A Day, POB 2A-SQN G. R. Signer, WT 6A-K S. A Howard, OPS 5N-SQN M. D. Skaggs, OPS 4A-SQN K. Langdon, POB 2B-SQN K. M. Hodges (EDMS), LP 2V-C

PERMITTEE NAME/ADDRESS (Include Facility N ameA.ocalion if Different) NATIONAL POLLUTANT DISCHARGE EUMlNATION SYSTEM (NPDES) Fonn Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) ~~--~~SEOUOYA~UC~ARP~ -- - (SUBR 01) OM8No. 204~ .&IQ[!ID J...Q.JtDX 2.QOO_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _j iNTEROF~U>es*2.t-12QW _ _ _ _ _ - - --- ~@DY~AI~rn ~------- 1---P-;-R.~.. ; ~T.; .o~

                                                                                                                         .; .~6.u; .4 50 M..;;..

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                                                                                                                                                                       ~~M~ER I ~~;:~R               DISCHARGE

£~~ -WA~EQ~~~~~~~ ---- ~a~ ~~TO~@ _________ _ EFFLUENT ATIN: stephanie A. Howard I I 05 YEAR MO

                                                                                                                                                                                      ...,. NO DISCHARGE NOTE: Read instructions before complelin!l lhis loon X

PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE ex OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. CENTIGRADE SAMPLE MEASUREMENT

                                                                                                                                    -                                                           35.7               04        0     31 I 31       RCORDR 00010     1    0                             PERMIT REQUIREMENT
                                                                       ********                   *'***"****                      ****               ******"**           ...........        Req. Mon.           DEG.C.              CONTI        CALCTD EFFLUENT GROSS                                                                                                                                                                             DAILY MAX                               NUOUS TEMPERATURE, WATER DEG.                      SAMPLE MEASUREMENT
                                                                       *******""'                  ********                         ...               ********           ********               25.9               04        0     31 I 31       MODELO CENTIGRADE 00010     z    0                             PERMIT                    ********                    ********                                          ********            **"****                30.5            DEG.C.              CONTI        CALCTO REQUIREMENT INSTREAM MONITORING

_.............. DAILYMX NUOUS TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C SAMPLE MEASUREMENT

                                                                                                                                    -                 ****'****          ********                  2               04        0     31 / 31       CALCTO 00016     1 EFFLUENT GROSS s                            PERMIT REQUIREMENT
                                                                        *'*******                  ********                      -**                 ********            ********                  3 DAILY MX DEG. C.             CONTI NUOUS CALCTD FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT
                                                                        ********                    1644                            03                *"*******          ****"****            **"*-                ..        0     31 I 31       RCORDR 50050     1     0                            PERMIT REQUIREMENT
                                                                       .............            Req. Mon.                       MGD                   ******"*           ...........          .............       .....             CONTI        RCORDR EFFLUENT GROSS                                                                                DAILY MAX                                                                                                                            NUOUS CHLORINE, TOTAL RESIDUAL                     SAMPLE MEASUREMENT
                                                                        ********                   ********                          ..               ********            0.023                0.046               19        0     21 I 31        GRAB 50060     1 EFFLUENT GROSS 0                             PERMIT REQUIREMENT
                                                                        *****'***                  ********                        --                 ********              0.1 MOAVO 0.1 DAILY MAX MGJL            FIVE PER CALCTD WEEK TEMPERATURE - C, RATE OF                     SAMPLE                     ...............                 0                           62
                                                                                                                                                                                                                   **        0     31 I 31       CALCTO MEASUREMENT CHANGE 82234      1    0                             PERMIT REQUIREMENT
                                                                        *******"*                       2                       DEG CfHR
                                                                                                                                                                                                                  ....              CONTI        CALCTD EFFLUENT GROSS                                                                                  DAILYMX                                                                                                                            NUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT
                                                                                                                                                                 ~"~~~

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify Wlder penally of law lhallhis doCI.mem and al attachments were prepared Wlder my TELEPHONE DATE

                                              ...;. ection or supennsion in accordance With a system designed to assure that qualified personnel Michael D. Skaggs               properly galhef and evaluate lhe onfonnation s.bn*lled. Based on my Wlquiry ollhe person or persons who manage lhe system. or those persons directly responsible for gathering lhe irtormai!Dn, lhe onformation S<.tlmilled IS
  • lO lhe best of my knowledge and belief, true. aca.r.Jte, Pre*<eol 423 843-7001 11 06 07 Sequoyah S ite Vice President and complete I am aware that !here are s.gnlf>eant penalties for st.t>mitbng false infonnatior\ SIGNATURE OF PRINCIPAL EXECUTIVE I
                                               *ncluding lhe possibility of fine and irnprisom>enl for knowing violations.

TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT

                                                                                                                                                                                                            ~~~ I       NUMBER       YEAR MO          DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS /Reference all attael7ments here No dosed mode operation. Veliger monitoring data is induded as an attachment. The following injections occurred: 1. Towerbrom 960 2 . Floguard MS6236 (max. calc. cone. was 0.11 mg/L-Iimit 0.2mg/L) 3 . Spectrus CT1300 (max. calc. cone. was 0 .039mg/L-Iimit 0.050mg/L)

EPA Form 3320*1 (REV 3199) Previous editions may be used Page 1 of 1

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp.fC) Temp.fC) LOCATION Clams/m3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-ISV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB 02/08/2011 0 0 9 02/08/2011 0 9 1-25-545 MJW 02/15/2011 0 0 23 02/15/2011 0 23 1-25-545 MLW 02/22/2011 20 100 10 02/22/2011 0 10 1-25-545 PB 03/01/2011 0 0 11 03/01/2011 0 11 1-ISV-24-1236 PB 03/08/2011 0 0 11 03/08/2011 0 11 1-ISV-24-1236 WE 03/16/2011 22 0 11 03/16/2011 0 11 1-ISV-24-1234 MLW 03/23/2011 0 0 11 03/23/2011 0 11 1-ISV-24-1234 MLW 03/30/2011 0 0 12 03/30/2011 0 12 1-15v-24-1236 MLW 04/06/2011 18 100 15 04/06/2011 0 15 1-ISV-24-1234 HMW 04/08/2011 45 100 15.5 04/08/2011 0 15.5 1-1SV-24-1236 WAW/PB 04/20/2011 21 100 16 04/20/2011 0 16 1-1SV-24-1236 PB May 2011 No Samples Collected

PERMITTEE NAME/ADDRESS (Include Facifity Name/location if Dffferentl NATIONALPOLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESI MAJOR Fonn Approved. DISCHA.RGE MONITORING REPORT (DMRJ Nam~ -W~SEOUOY~~UC~ARP~~ --- (SUBR 01) OMB No. 2040-0004 Address _p....Q.JlOX 2000_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _(INTEROFFICE OPS*SN*SQN! _ _ _ _ _ _ _ TN0026450 101 T F - FINAL --- ~ODDY~AISY. TN373M_ _ _ _ _ _ _ _ ERMIT N UM BE DI SCHARGE NUMBE BIOMONITORING FO R OUTFAL L 101 F~ili~ _WA~~@~HN~LEARP~N~ --- EFFLUENT Lo~o~AMI~N COUN~-- -- --- - -- r:~T9RING e;:!~9 ATTN: stephanie A. Howard I I 05 [ 01 ] YEAR MO To r 11 MQ I 05 QAY 31

                                                                                                                                                                                 ~    NO D ISCHARGE        D ...

NOTE: Read instructions before completin~ this form. X PARAMETER QUANTITY OR LOADING OUAUTY OR CONCENTR.ATION NO. FREQUENCY SAM PLE EX OF ~PE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANAlYSIS IC25 STATRE 7 DAY CHR SAMPLE MEASUREMENT

                                                                                                                              -                >100.0              ********            ********            23         0      1 / 180     COMPOS CERIODAPHNIA TRP38      1 E FFLUENT G R OSS 0                               PERMIT REQUIREMENT
                                                                           ****:I:***                 ********               --                  43.2 MINIMUM
                                                                                                                                                                  ********             **'******       PERCENT                S E MI ANNUAL COMPOS IC25 STATRE 7DAY CHR PIMEPHALES SAMPLE MEASUREMENT
                                                                                                                              -                >1 00.0             ********            ********            23         0      1 / 180     COMPOS TRP6C      1 EFFLUENT GROSS 0                               PERMIT REQUIREMENT
                                                                           ********                   ********               -                   43.2 MIMINUM
                                                                                                                                                                   *****'*"*'*         ******'**       PERCENT                S EMI ANNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMrT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TlnE PRINCIPAL EXECUTIVE OFFICER I Certify ooder penalty ollaw lhat this documenl and aN attachments were prepared under my                                                                        TELEPHONE                    DATE Michael D . Skaggs Sequoyah Site Vice Preside nt direction or s....,e<vision in aoconlance Yoith a system designed lo asS\Jre lhat qualified personnel properly gather and evaluate lhe infonnation submitted. Based on my inqtjry or the person or pe1SOOS who manage lhe system, or lhose persons diredJy responsible for galhering lhe inlonnalion, lhe information Slbnitted is
  • to lhe best of my l<nowledge and belief, true. accurate, and complete. I am a"Nare lhat there are Signi!icari penalties for sOOmilling false information, Q,?I:Wre, ..,,

SIGNATURE OF PRINCIPAL EXECunVE 423 I 843-7001 11 06 07 including the possibility or f1<1e and ~riSOMlenl ror knowing violations.

                                                                                                                                                                                                     ~~ ~

OFFICER OR ALITHORIZED AGENT NUMBER YEAR MO DAY

              ~PEO   OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS                (ReFerence all attachments here Toxicity sampling began o n May 1 and ended on May 6. The toxicity report is induded.

EPA Fonn 3 3 20-1 (REV 3199) Previous editions may be used Page 1 of 1

June 3, 2011 Bradley M. Love~ OPS 5N-SQN SEQUOY AH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, MAY, 2011 Per your request, I have also submitted an electronic copy of the subject report. Outfall J0 I samples collected May 01-06, 2011, showed no toxic effects to fathead minnows or daphnids. The resulting IC2s values for both species were> I 00 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfaii 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mmtality occurred in minnows exposed to non-treated and UV -treated samples. Please call me at your convenience if you have any questions or comments following your review of the report. Donald W. Sno grass Manager (Acting), Environmental Engineering Field Team-Muscle Shoals CTR2L-M Attachment Cc (Attachment): R.M. Sherrard, PSC IX- C (Electronic copy) EDMS, MPB IE - M (Electronic copy)

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: June 03,2011

1. Facility I Discharger: Seguoyah Nuclear Plant I TVA
2. County I State: Hamilton I Tennessee
3. NPDES Permit#: TN0026450
4. Type of Facility: Nuclear-Fueled Electric Generating Plant
5. Design Flow (MGD): 1.579
6. Receiving Stream: Tennessee River (TRM 483.6)
7. 1Q10: 3.491
8. Outfall Tested: 101
9. Dates Sampled: May 01 - 06, 2011
10. Average Flow on Days Sampled (MGD): 1635.7, 1623.12, 1618.58
11. Pertinent Site Conditions: Production I operation data will be provided upon request.
12. Test Dates: May 03- 10,2011
13. Test Type: Short-term Chronic Definitive
14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested(%): Outfall101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100.0 Pimephales promelas: UV treated Outfall101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100.0
16. Permit Limit Endpoint(%): Outfall101: IC22. = 43.2%
17. Test Results: Outfall 101: Pimephales promelas: Kas > 100%

Ceriodaphnia dubia: Kas > I 00% UV treated Outfall 101 : Pimephales promelas: Kz 5 > 100% Page 1 of 100

18. Facility

Contact:

Brad Love Phone#: (423) 843-6714

19. Consulting I Testing Lab: Environmental Testing Solutions. Inc.
20. Lab

Contact:

Jim Sumner Phone#: (828) 350-9364

21. TVA

Contact:

Donald W. Snodgrass Phone#: (256) 386-2787

22. Notes: Outfall101 samples collected May 01-06, 2011, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >

100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 2 of 100

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall I 01, Intake
2. Sample Type: Composite
3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (oC) (mg/L) LastUsedBv 05-01-11 0800 to 05-03-11 1415 101 05-02-11 1512 o.7, o.8t <0.10 05-02-11 0700 05-04-11 1318 05-01-11 0845 to 05-03-11 1415 Intake 05-02-11 1512 1.2 <0.10 05-02-11 0745 05-04-11 1318 05-03-11 0800 to 05-05-11 1319 101 05-04-11 1437 0.7, 0.9t <0.10 05-04-11 0700 05-06-11 1317 05-03-11 0830 to 05-05-11 1319 Intake 05-04-11 1437 0.8 <0.10 05-04-11 0730 05-06-11 1317 05-07-11 1319 05-05-11 0800 to 0.9, 0.9, 101 05-06-11 1515 <0.10 05-08-11 1322 05-06-11 0700 1.5tt 05-09-11 1316 05-07-11 1319 05-05-11 0830 to Intake 05-06-11 1515 0.9 <0.10 05-08-11 1322 05-06-11 0730 05-09-11 1316

  *TRC =Total Residual Chlorine tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

ttsamples were collected in two 2.5 gallon cubitainers and one 5 gallon cubitainer. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfalll01 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath.

Aliguots ofOutfalliOI and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics. Inc.) for 2 minutes. Page 3 of 100

Pimephales promelas Ceriodaphnia dubia Test Organisms:

1. Source: Aquatox, Inc. In-house Cultures
2. Age: 21.75-22.25 hours old < 24-hours old Test Method Summary:
1. Test Conditions: Static, Renewal Static, Renewal
2. Test Duration: Until at least 60% of control females have 3 broods
3. Control I Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
4. Number of Replicates: ~
5. Organisms per Replicate: 10 1
6. Test Initiation: (Date/Time)

Outfall101 05-03-11 1415 ET 05-03-11 1315 ET UV Treated Outfall 101 05-03-11 1345 ET

7. Test Termination: (Date/Time)

Outfall101 05-10-11 1325 ET 05-10-11 1224 ET UV Treated Outfall 101 05-10-11 1342 ET

8. Test Temperature: Outfall101: Mean = 24. 7°C Mean= 25.0°C (24.2- 25.2°C} (24.7- 25.2°C}

Test Temperature: UV-Treated Outfall101: Mean = 24.8°C (24.2- 25.2°C}

9. Physical I Chemical Measurements: Alkalinity, hardness. total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville, CA}.

Page 4 of 100

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephales promelas Chronic/7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted May 03- 10, 2011 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used - days) (%Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 98 98 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 98 95 93 Mean Dry Weight (mg) Test Solutions (replicate number) (%Effluent) 1 2 3 4 Mean Control 0.704 0.756 0.799 0.819 0.770 10.8% 0.849 0.889 0.898 0.768 0.851 21.6% 0.659 0.886 0.913 0.879 0.834 43.2% 0.761 0.844 0.841 0.853 0.825 86.4% 0.845 0.805 0.716 0.797 0.791 100.0% 0.801 0.696 0.863 0.800 0.790 Intake 0.893 0.794 0.774 0.660 0.780 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.2% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

     *TUa = 1OOILC5o: TUc = 100/ IC2s Page 5 of 100

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted May 03- 10,2011 using effluent from Outfall101. Percent Surviving Test (time interval used- d<!Y_s) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 28 28 31 31 31 31 30 29 29 29 29.7 10.8% 33 30 34 32 27 33 30 28 30 32 30.9 21.6% 33 32 31 36 29 32 31 31 31 33 31.9 43.2% 36 32 34 30 35 36 35 34 32 34 33.8 86.4% 34 35 33 35 36 37 36 35 33 35 34.9 100.0% 38 36 36 37 35 38 36 36 36 36 36.4 ICz5 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.2% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

     *TUa = 100/LCso: TUc = 100/ ICzs Page 6 of 100

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted May 03- 10,2011 using water from Intake Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 26 34 30 29 27 30 32 31 29 29.7 Intake 37 37 35 35 37 35 32 36 38 36 35.8 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

     *TUa = 100/LCso: TUc = 100/ IC2s Page 7 of 100

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type I Duration) Conducted May 03- 10, 2011 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used - days) (%Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 98 98 98 86.4% 100 100 100 100 98 98 98 100.0% 100 100 98 98 98 98 98 Intake 100 100 100 100 100 100 98 Mean Dry Weight (mg) Test Solutions replicate number) (%Effluent) 1 2 3 4 Mean Control 0.714 0.908 0.853 0.849 0.831 10.8% 0.901 0.813 0.867 0.888 0.867 21.6% 0.896 0.793 0.779 0.829 0.824 43.2% 0.879 0.852 0.847 0.773 0.838 86.4% 0.850 0.770 0.774 0.797 0.798 100.0% 0.816 0.802 0.660 0.840 0.780 Intake 0.948 0.883 0.925 0.799 0.889 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

      *TUa= 100/LCso: TUc= 100/IC2s REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant ResultsCIC2J Pimephales promelas May 03- 10,2011 1445 7-days KCl 0.78 giL Ceriodaphnia dubia May 03- 10,2011 1255 7-days NaCl 1.07 giL Page 8 of 100

PHYSICAUCHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges fur Pimephales promelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outmn 101 perfunred May03-10, 2011. Test Sample ID Temperature ("C) Dissolved Oxygen (mg/L) pH(S.U.) Conductance Alkalinity Hardness Total Residual Initial Flnal Initial Flnal Initial F1nal (Jlmhos/cm) (mg/L CaC03) (mg/L CaC03) Chlorine (mg/L) 24.8 24.6 7.6 7.7 7.81 7.77 321 59 90 Control 24.7 - 24.9 24.4 - 24.8 7.4 - 7.7 7.5 - 7.9 7.73 - 7.89 7.68 - 7.86 305 - 333 58 - 59 89 - 91 24.9 24.6 7.8 7.7 7.85 7.73 299 10.8% 24.7 - 25.0 24.2 - 24.8 7.5 - 8.2 7.3 - 8.0 7.80 - 7.93 7.64 - 7.87 283 - 311 1~ 1:1, 21.6% 24.9 24.7 - 25.0 24.9 24.6 24.4 - 24.8 7.7 - 7.9 8.1 7.3 7.7

                                                                           -   8.0 7.85 7.79 - 7.93 7.73 7.64 - 7.86 282 272 - 292 24.6            7.8           7.7          7.83          7.73         249
    ~           43.2%
     '::1                 24.7 - 25.0  24.5 - 24.8     7.7 -    8.1  7.4   -   8.1 7.77 - 7.92   7.66 - 7.86  241 - 257
    ~

s.. 86.4% 24.9 24.5 7.8 7.7 7.80 7.71 188

    .§                    24.7 - 25.1  24.4 - 24.6     7.7 -    8.0  7.4   -   8.1 7.72 - 7.92   7.66 - 7.85  179 - 207
    ~

25.0 24.5 7.9 7.7 7.79 7.71 163 58 63 <0.10 100.0% 24.9 - 25.2 24.3 - 24.7 7.7 - 8.1 7.4 - 8.0 7.71 - 7.90 7.64 - 7.85 157 - 174 54 - 61 55 - 71 <0.10 - <0.10 24.9 24.4 7.9 7.7 7.78 7.70 164 59 62 <0.10 Intake 24.7 - 25.1 24.3 - 24.6 7.8 - 8.1 7.3 - 8.0 7.69 - 7.92 7.64 - 7.87 155 - 178 55 - 61 55 - 67 <0.10 - <0.10 24.9 25.0 7.6 7.7 7.81 7.84 321 59 90 Control 24.8 - 24.9 24.7 - 25.2 7.4 - 7.7 7.4 - 8.0 7.73 - 7.89 7.77 - 7.90 305 - 333 58 - 59 89 - 91 25.0 25.0 7.8 7.7 7.85 7.82 299 10.8% 24.9 - 25.0 24.8 - 25.2 7.5 - 8.2 7.5 - 8.0 7.80 - 7.93 7.71 - 7.90 283 - 311

    -c:.                      25.0          25.0            7.9           7.8          7.85          7.84         282
    .§          21.6%

24.9 - 25.0 24.8 - 25.2 7.7 - 8.1 7.6 - 8.0 7.79 - 7.93 7.77 - 7.91 272 - 292 1:: 25.0 25.0 7.8 7.8 7.83 7.84 249

    ~           43.2%
     §-                   24.9 - 25.0   24.8 - 25.1    7.7 -    8.1  7.6 - 8.0     7.77 - 7.92   7.76 - 7.90  241 - 257
    ~

25.0 25.0 7.8 7.8 7.80 7.84 188

    *~          86.4%

24.9 - 25.1

    ~                                   24.8 - 25.2    7.7 -    8.0  7.6 - 8.0     7.72 - 7.92   7.74 - 7.93  179 - 207 25.1          25.0            7.9           7.8          7.79          7.83         163         58               63           < 0.10 100.0%

24.9 - 25.2 24.8 - 25.2 7.7 - 8.1 7.5 - 8.0 7.71 - 7.90 7.72 - 7.93 157 - 174 54 - 61 55 - 71 <0.10 - <0.10 24.9 24.9 7.9 7.8 7.78 7.82 164 59 62 <0.10 Intake 24.9 - 25.1 24.8 - 25.2 7.8 - 8.1 7.6 - 8.1 7.69 - 7.92 7.71 - 7.91 155 - 178 55 - 61 55 - 67 <0.10 - <0.10 Overall temperature ("C) Average Minimum Maximum Pimephales promelas 24.7 24.2 25.2 Ceriodaphnia dubia 25.0 24.7 25.2 Page 9 of 100

PHYSICAUCHEMICAL

SUMMARY

Water Chemistry Mean Vahres and Ranges fur Pimephales promelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outmll 101 perfunned May 03-10, 2011. Test Sample ID Temperature \C) Dissolved Oxygen (mg/L) pH(S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (Jlmhos/cm) (mg/L CaC03) (mg!L CaC03) Chlorine (mg/L) 24.9 24.6 7.8 7.7 7.83 7.74 311 59 92 Control 24.8 - 24.9 24.4 - 24.9 7.5 - 8.1 7.5 - 8.0 7.78 - 7.91 7.66 - 7.84 299 - 326 57 - 60 90 - 96 24.9 24.6 7.8 7.7 7.85 7.75 297 10.8% 24.9 - 25.0 24.3 - 24.8 7.7 - 8.1 7.3 - 8.0 7.79 - 7.92 7.66 - 7.85 286 - 310

     .i                     25.0          24.6           7.8            7.7          7.86          7.74        283
       ~       21.6%

24.9 - 25.1 24.2 - 24.8 7.7 - 8.1 7.3 - 7.9 7.80 - 7.93 7.66 - 7.86 270 - 293

       ~

l:l. 25.0 24.6 7.9 7.6 7.85 7.73 250 J!'::1 43.2% 24.9 - 25.1 24.5 - 24.7 7.7 - 8.1 7.3 - 7.9 7.79 - 7.91 7.65 - 7.86 240 - 256

       ~                    25.0          24.6           7.9            7.7          7.83          7.71        187 86.4%
     .§
     =...

24.9 - 25.1 24.5 - 24.7 7.8 - 8.1 7.4 - 8.0 7.75 - 7.90 7.62 - 7.87 178 - 197 25.1 24.6 8.0 7.7 7.81 7.69 164 57 69 <0.10 100.0% 24.9 - 25.2 24.5 - 24.7 7.9 - 8.1 7.4 - 7.9 7.73 - 7.91 7.62 - 7.86 154 - 178 52 - 61 63 - 76 <0.10 - <0.10 25.0 24.6 8.0 7.8 7.79 7.70 164 58 69 <0.10 Intake 24.8 - 25.1 24.4 - 24.7 7.9 - 8.2 7.5 - 8.0 7.71 - 7.90 7.63 - 7.85 155 - 175 52 - 63 63 - 76 <0.10 - <0.10 OveraU temperature \C) Average Minimum Maximum Pimephales promelas 24.8 24.2 25.2 Page 10 of 100

SUMMARY

I CONCLUSIONS OutfalllOI samples collected May 01-06,2011, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall I 01 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 11 of 100

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None
2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
1. Pimephales promelas None
2. Ceriodaphnia dubia None Page 12 of 100

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
3. Temperature was measured by SM 2550 B.
4. Dissolved oxygen was measured by SM 4500 0 G.
5. The pH was measured by SM 4500 H+ B.
6. Conductance was measured by SM 2510 B.
7. Alkalinity was measured by SM 2320 B.
8. Total hardness was measured by SM 2340 C.
9. Total residual chlorine was measured by ORION Electrode Method 97-70.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in giL KCl or NaCl.
2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.
4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 13 of 100

REFERENCES

1. NPDES Permit No. TN0026450.
2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
3. Standard Methods for the Examination of Water and Wastewater, 21st Edition, 2005.
4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 14 of 100

Sequoyah Nuclear Plant Biomonitoring May 03- 10,2011 AppendixB Diffuser Discharge Concentrations of Total Residual Chlonne, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 100

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- May 6, 2011 Page 16 of 100

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- May 6, 2011 Date PCL-401 mg!L Copolymer 0.009 0.028 0.009 0.009 0.009 0.009 0.009 0.019 0.019 0.018 0.019 0.019 0.019 0.019 0.020 0.020 0.020 0.020 0.020 0.020 0.020 08/26/2001 0.021 08/27/2001 0.021 08/28/2001 0.021 08/29/2001 0.020 08/30/2001 0.021 08/3112001 0.020 11/25/2001 11126/2001 0.02 11127/2001 0.019 11128/2001 0.019 11129/2001 0.02 11130/2001 0.02 12/09/2001 12/10/2001 12/1112001 12/12/2001 0.02 12/13/2001 0.02 12/14/2001 0.02 Page 17 of 100

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- May 6, 2011 Date PCL-401 mg/L Copolymer 01/02/2002 0.02 01103/2002 ' 0.014 01/04/2002 0.014 01105/2002 01106/2002 01/07 0.014 0.023 0.023 0.023 0.008 0.008 05/05/2002 05/06/2002 0.02 05/07/2002 0.02 05/08/2002 0.019 05/09/2002 0.02 05/10/2002 0.019 08/04/2002 08/05/2002 0.018 08/06/2002 0.018 08/07/2002 0.019 08/08/2002 0.019 O.o18 O.o18 O.o18 O.o18 O.o18 O.ol8 0.019 0.020 0.020 0.020 0.009 0.021 0.021 0.021 0.021 0.022 Page 18 of 100

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth ofMicrobiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998- May 6, 2011 Date PCL-401 mg/L Copolymer 0.020 0.014 0.014 0.020 0.020 08/03/2003 08/04/2003 0.020 08/05/2003 0.020 08/06/2003 0.020 08/07/2003 0.020 08/08/2003 0.020 10/05/2003 0.020 10/06/2003 0.020 10/07/2003 0.020 10/08/2003 0.020 10/09/2003 0.022 10/10/2003 0.024 02/01/2004 0.009 02/02/2004 ; 0.009 02/03/2004 0.009 02/04/2004 . 0.009 02/05/2004 0.009 02/06/2004 0.009 05/04/2004 0.019 05/05/2004 0.014 05/06/2004 0.013 05/07/2004 0.020 05/08/2004 0.021 05/09/2004 0.020 0.019 0.020 0.020 0.019 0.019 0.020 Page 19 of 100

Table B-I. Sequoyah Nuclear Plant Diffuser (Outfall I 0 I) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, I998- May 6, 20II Date 11/07/2004 11108/2004 11/09/2004 11/10/2004 11/11/2004 1111 02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/1112005 06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10/2005 07117/2005 07/18/2005 07/19/2005 07/20/2005 07/21/2005 07/22/2005 10/30/2005 10/3112005 1110112005 11102/2005 11103/2005 11104/2005 11114/2005 11115/2005 11116/2005 11117/2005 11118/2005 11119/2005 Page 20 of 100

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall I 0 I) Discharge Concentrations of Chemicals Used to Control Growth ofMicrobiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, I998- May 6, 20II Date Cuprostat-PF MSW mg/L 101 Azole mg/L 11112/2006 11113/2006 11114/2006 11115/2006 11116/2006 11117/2006 11126/2006 11/27/2006 11128/2006 11129/2006 11130/2006 12/0112006 05/28/2007 0.015 05/29/2007 O.Q15 05/30/2007 O.o15 05/3112007 O.Q15 06/0112007 0.015 06/02/2007 O.Q15 12/02/2007 12/03/2007 12/04/2007 12/05/2007 12/06/2007 12/07/2007 04/13/2008 04/14/2008 04/15/2008 04/16/2008 04/17/2008 04/18/2008 10/26/2008 10/27/2008 10/28/2008 10/29/2008 0.030 10/30/2008 0.030 10/3112008 0.030 Page 21 of 100

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall lO I) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 20 I I Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-I 30M Nalco Spectrus H-150M MSW Hypochlorite mg!L 222 mg/L mg/L -PF mg/L mg!L 73551 CT1300 mg/L 101 mg/L TRC mg!L Copolymer DMAD Azole Quat mg!L mg/L Quat mg/L TRC Phosph EO/PO Quat Phosphate ate 02/08/2009 - 0.0197 - - - - - 0.017 - - - 02/09/2009 - 0.0237 - - - - - 0.017 - - - 02110/2009 - 0.0104 - - - - - 0.021 - - - 02/11/2009 - 0.0155 - - - - - 0.017 - - - 02/12/2009 - 0.0106 - - - - - 0.017 - - - 02/13/2009 - - - - - - - - - - - 05/ 10/2009 - 0.0129 - - - - - - - - - 05/ 11/2009 - 0.0415 - - - - - - - 0.0446 - 05/ 12/2009 - 0.0053 - - - - - - - 0.0396 - 05/ 13/2009 - 0.0049 - - - - - - - 0.0396 - 05/ 14/2009 - <0.0141 - - - - - - - 0.0397 - 05/ 15/2009 - <0.0160 - - - - - - - - - 1111 5/2009 - 0.025 - - - - - - - - - 11116/2009 - 0.0152 - - - - - - - - - 11/ 17/2009 - 0.0255 - - - - - - - - - 11/ 18/2009 - 0.0306 - - - - - - - - - 11/1912009 - 0.0204 - - - - - - - - 11/20/2009 - 0.0093 - - - - - - - - - 05/09/2010 - 0.0192 - - - - - - - - - 05/ 10/2010 - 0.0055 - - - - - - - - - 05/ 11/2010 - 0.0100 - - - - - - 0.039 - - 05/12/2010 - 0.0171 - - - - - - 0.039 - - 05113/2010 - 0.0041 - - - - - 0.039 - - 05/14/2010 - 0.0099 - - - - - - 0.039 - - Page 22 of 100

Table B- 1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control M icrobiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Date Soclium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 mg/L TRC mg!L Copolymer DMAD Azole Quat mg/L mg/L Quat mg/L TRC Phospb EO/PO Quat Phosphate ate 10/31/2010 - - - - - - - - - - - 11/01/2010 - 0.0 122 - - - - - - - - - 11/02/2010 - O.Ql12 - - - - - - - - - 11/03/2010 - 0.01 63 - - - - - - - - - 11/04/2010 - 0.0107 - - - - - - - - - 11/05/2010 - 0.0132 - - - - - - - - - 05/01/2011 - - - - - - - - - - - 05/02/2011 - - - - - - - - 0.04 - - 05/03/2011 - - - - - - - - 0.04 - - 05/04/20 11 - 0.0155 - - - - - - 0.04 - - 05/05/20 11 - 0.0179 - - - - - - 0.04 - - 05/06/2011 - 0.0089 - - - - - - - - - Page 23 of 100

Sequoyah Nuclear Plant Biomonitoring May 03-10,2011 AppendixC Chain of Custody Records and Toxicity Test Bench Sheets I1 1i ll

~

Page 24 of 100

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 ofl Client: TVA Environmental Testing Solutio~ Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery

                                                                                                                                . General Comments:

Facility Sampled: Sequoyah NP 28801 Ray Duncan: NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell 1 Sara Snyder.

                                                                    /Fax:                 828-3 50-93 68                              Metals samples filtered and stored. Samples remained on

. l e t By: Ray~~er __ AL-" ice through out sampling and transport to Jab. CV~comp. -/, ~ . ..

   "'eld Identification I                            Collection Date/Time            Container     Flow Rain Event?

fi\Oj Rt:t'1-ao8 ' Sample Description Number& MGD Laboratory Use Volume (Mark as Appropriate) . 'i Collected - ~"\~-drut10 ~'~~~l:-%i:\~~~:~t~i ~~~1~

                                                                                                 ~~~l Date               Tune                              Yes      If Yes,      No      Trace       IITS Log     Arrival      By      Tune

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,,;: _ ' ' . * . -~'-'1 ~ti E-r ,/ - (OC) £1"" 1 SQN-10 1-TOX Comp 5/J/II - S/<J.!,, oUtt'-c7a 2 (2.5gal) 16~.'1 / lO!bl.OI o.1.o.8'C ~ . .c;iV \(:.~ ,*:: SQN-INTAKE- Comp I (2.5 gal) NA ";k - , TOX s/1111 -s-/OV,! ~ts:"7~

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Sample Custody- Fill In From Top Down _.,. c~~~ '."!!'~;.~~c.e;. ~~

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Relinquished By (Signature): Dateffime Received By (Signature): effrme

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                                                 -Nf\          05/0211 l lt>F7         £\       Sonic Delivery If!~          .JGI. L                        05/02111
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                                                                                                          '-./f Instructions: Clients should fiiJ in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the foUowing Monday.

Page 25 of 100

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                                  .     ,i Wbole Emuent Toxicity Sample Receipt Log Page_i\

Dale Tlrue Received Received Sample Project Sample Sample aame aad desuiptioa State Commeats received n:ceived by from temperature ("C) number aamber 05-m-11 1512 J. Sumner TVA Courier 0.7 0.8 7068 110502.01 TVA Sequoyah Nuclear Plant- 101 TN 05-m-ll 1512 J. Sun>ne< TVA Courier 1.2 7068 110502.02 TVA

  • Sequoyah Nuclear Plant -Intake TN Page 26 of 100

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1oft Client TVA Environmental Testing Solution, Inc. Delivered By (Circle One): - Project Name: Sequoyah NP Toxicity 351 Depot Street Fed.Ex UPS Bus Client P.O. Number: N/ A Asheville, NC Other (specify): Sonic Delivery 28801 General Comments: Facility Sampled: Sequoyah NP Ray Duncan: NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell Sara Snyder: ~rl1sy, ~-~~,,~ Fax: 828-3 50-93 68 Metals samples filtered and stored. Samples remairied on ice through out sampling and transport to lab. a-. /l17L ,.:;::_ ~- - - Fjld Identification I tlra&Comp. Container Flow l../'?/Collection Dateffime Rain Event? p~id"~ Sample Description Number& Volume MGD 'lDaQ Laboratory Use . .. . Collected (Mark as Appropriate) . ~.;;~><~11 .. "'Jffii>.il£.. Date " *-;:-;t)f~* If Yes, No

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Relinquished By (Signature): Date/Time Received By {Signature): IN "-\L r.Ntll.ftate/Time d '-*

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Sonic Derfvery 05/04111 ETS 05/04/ 1I t'-13\ E\ / '\""'"/ (Mt * - 1'l31 Er

                                                                                                                    '-./   t Instructions: Clients should fill in all areas except those in the "Laboratory Use"" block. Biomonitoring samples are preserved by storing them at 6"C and shipping them in ice. The hold time for ea.ch sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time ftame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Page 27 of 100

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~---- Date r ... e Received Samp~("C)  ::: ~.!: [SaJDPi2WIIUe aad description State CoiBDlOJlla received received from 05 0955 Fee 1.4 7076 110504.01 IDuke' creel< 05 09! Fee .3 707< 110504.02

  *o5         0955                 :eenan  Fee             .2        7078  110504.03 Proams                             ~B.

05 0955 :eenan Fee 1.2 7078 110504.04 ProJUeSS ~B.- 05 0955 :eenan Feel-Ex 1.0 7079 10504.05 *- i>.l iiiSWWTP 05-04-1 0955 .Keenan Feel-Ex 25 7080 10504~116 Uruted 1.iil.r~ IWWll' 05-04-1 0955 .Keenan Fed -Ex 2.5 7081 110504.07 United Water- !k:odand Neck WWTP 05-04-1 0955 . Keenan Feel- Ex 1.0 708: 10504.08 NC 05-04-1 0955 . Keenan Feel-Ex 1.0 7083 10504.09- Efeffien~ NC 05-04-11 0955 .Keenan Fed-Ex 1.0 7084 110504.10 UnitedWater- NC 05-04-1 0955 Keenan Fed-Ex 25 7085 110504. ProeieSSEn.r.!VCaroi~ NC 05-04-1 0955 K. Keenan Fed-Ex .4 7086 II 0504.12 Pro...-ess Bneri<V Carolinas - Shearon Harris B&B Center NC 05-04-11 0955 K. Keenan Fed -Ex 0.9 7087 110504. Carolinas -Shearon -Harris Plant NC 05-04-11 0955 K. Keenan Fed-Ex 0.6 7088 110504.1*f ~ 05-04-11 1000 K. Keenan UPS 0.5 7089 II 0504.15 Microbac I 1- I , WWTP NC 05-04-11 1()(}!! K. Keenan UPS 0.5 7090 110504.16 Microhac I 05 1000 K Keenan UPS 05 7091 I10504 10 Mir=h=. . IP "'p*-* Wm.ram WWTP 05-04-1 1000 K. Keenan UPS a: 709:i 110504.18 Mn* .ead ~itvWWTP Nt 05-04-1: 1000 K. Keenan IPS 0.6 7093 IIOS04.f9 *-Enl!eii)ard\1/TP Nt 05 !26 K Keenan Dash Courier 05 7094 I10504 ?0 m* lA . Moonn I'*~* WWTP NC 1431 .Sumner TVA Courier 07/09 7068 110504 N".

  • Senunvoh Nuolear Phmt. 10 IN 05-04-1 1437 .Sumner TVA Courier 08 7068 110504 ;v,
  • Seouovah Nuolear Plant* Intake IN SOP 04 - Exhibit 04.2, revision 06-29-09 Page 28 of 100

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 ofl Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One4 Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): 28801 General Comments: Facility Sampled: Sequoyah NP Ray Duncan:_ __ __ _ _ _ __ _ _ NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell SrurnSnyder:.~~-------~---- Fax: 828-350-9368 ' Metals samples filtered and stored. Samples remained on ice through out sampling and transport to lab. Container Flow Number& MGD Rain Event? Laboratory Use (Mark as Appropriate) If Yes. No Trace ETS_Log Inches Number SQN-INTAKE- Comp TOX Relinquished By (Signature): 05/0611 I Sonic Delivery .,1 05/06/11 Sonic 05/06111 ETS 05/06111 _J:*j_r- ET IS Instructions: Clients should fill in all areas except those in the '"'Laboratory Use~ block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. Page 29 of 100

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                                                                                                                                                                                            -J Page_.J!l Whole Emuent Toxicity Sample Receipt Log Dale               Time       Received                 Received      Sample       Project  Sample   Sample Dllllle aad dacriplioa                              State           CoBUBeJlts received           I"Keived         by                       from  lempe<alure ("C) aumber   number OS-06-11             0946       K.Keenan                     UPS          3.3        7089   110506.01 Microbac Environmental - Roc:kingham WWTP                   NC OS-06-11             0946       K. Keenan                    UPS          3.3        7090   110506.02 Microbac Environmental -Roseboro WWTP                       NC OS-06-11             0946       K.Keenan                     UPS          3.3        7091   110506.03 Microbac.Environmental- Westooint- w......... WWTP          NC OS-06-11             1000       K.Keenan                  Fed-Ex          1.9        7076   110506.04 Duke Energy Carolinas - Belews Creek Sleam Station          NC OS-06-11             1000       K.Keenan                  Fed- Ex         2.3        7077   110506.05 Bladenboro WWTP                                             NC OS-06-11             1000       K.Keenan                  Fed-Ex          2.S        7078   110506.06 ~EnergyCarolinas- Cane Fear S.E.                            NC 05-06-11             1000       K.Keenan                  Fed-Ex          2.S        7078   110506.07 Prowess Enenw Carolinas - Cepe Fear S.E. - Upstream/lntake  NC OS-06-11              1000      K.Keenan                  Fed-Ex          2.7        7079   110506.08 Citv of Gastonia - Dallas WWTP                              NC 05-06-ll              1000      K. Keenan                 Fed-Ex          2.0        7080   110506.09 United Wa!er- Enfield WWTP                                  NC 05-06-11              1000      K. Keenan                 Fed-Ex          2.0        7081   110506.10 United Water- Scotland Neclc WWTP                           NC OS-06-11              1000      K. Keenan                 Fed-Ex          0.8        7082   110506.11 WilaonWWTP                                                  NC OS-06-11              !000      K.Keenan                  Fed-Ex          1.8        7083   110506.12 Elementis                                                   NC OS-06-l!              1000      K.Keenan                  Fed- Ex         0.9        7084   110506.13 United Water- Farmville WWTP                                NC 05-06-11              1232      K. Keenan             Dash Courier         1.3       7094   110506.14 OWASA- Mason Farms WWTP                                     NC 05-06-11              ISIS      J. Sumner             TVA Courier     0.9,0.9, I.S   7068   110S06.1S TVA-se<luo'WilNuclearPiant-101                              1N OS-06-11              ISIS      J. Sumner             TVA Courier         0.9         7068  IIOS06.16 TVA - Sequovah Nuclear Plant- Inlllke                       1N SOP 04 - Exhibit 04.2, revision 06-29-09 Page 30 of 100

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Page I of6 I Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas I Client: Tennessee Valley Authority Facility: S~uovah Nuclear Plant NPDES #: ~66!6~ TN()()'Ve"l~O County: ~ t-\f\to\\\.."ttN Outfall: 101 I Project#: _......:,"""0'""\o:,_,&,.____ _ __ Dilution preparation information: Comments: I Dilution prep (%) Effiuent volume (mL) Diluent volume (mL) 10.8 270 2230 21.6 540 1960 432 1080 1420

                                                                                  '  86.4 2160 340 100 2500 0

Total volume (mL) 2500 2500 2500 2500 2500 I Test organism information: Test information: Organism age: '1."2..'2..S - . 71 tlo.Jrt.":, _0\.b Randomizing template: '-1-l-LLOw I Date and times organisms were born between: os*<n.*t\ \\..00 Incubator number and shelf location: ..3(. Organism source: Artemia CHM number: CHti~ ATO-t. &A-u..~ l>f o~ *en-* \ I Drying information for weight determination: Transfer bowl information: pH = , .\o 8' s.u. Date I Time in oven: lo_'ft.IOo\ \ 1'-liO I Average transfer volwne: Temperature = 1."l."'L oc Initial oven temperature: Date I Time out of oven: O~*ll*f\

                                                                                                                                          \oO *(
o. l11(o~ Final oven tell'l2_erature: lo~*l I Daily feeding and renewal information:

Total drying time: 1."'*1411JI2S I Day Date Morning feeding Afternoon feedin g Test initiation, renewal, or Sample numbers used MHSW batch 0 2 3 4 I 5 6 I 7 I ' Page 31 of 100 SOP AT20 - Exhibit AT2.0.3, revision 04-0l-09

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I .) Env!ranmantal 'll!sllng Salutionr,lnc. I Species: Pimeohales promelas I Client: TVA I Sequoyah Nuclear Plant, OutfalllOl, Non-treated s... ..

  • and Growth Data Date: OS-~*1 \

-I Day A t:UNTKUL B c D E l0.8% F G H I 'J _21.6%_ _!{ L 0 10 /0 10 ID to -10 /0 IO /Q /0 10 tO I l

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IC> ID ro {CJ IC /(J I~ I~ /Q I~ l(J L..~\\'f Swt Tray color code::

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I Analyst: Date: B =Pan + Larvae weight (mg)

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I Hand calculated* Analyst: Weight per initial number of larvae (mg)

   = C /Initial number of larvae I                           .A/                .              ~::,..,  1\~      ,~    ~,t..     ~0...    \'bfl\     ~,.,t.    ,....o.o   \o~~     \:~ ~
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Q; ~ Analyst: r- o*"' o* o* o* t>' ()' o* ()* C)' ~* ()* Average weight per Percent reduction I initial number of larvae (mg) from control(%) o.'11b o.ss \ -l{).\.1. o.&~~ - g,'-{7. Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, I lg = unusually large, d&r = decanted and returned, w = wounded. Comments: I -I I Page 32 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

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I Species: Pimephales promelas I Client: TVA I Seauoyah Nuclear Plant, Outfall lOt, Non-treated Date: os. at.* t \ Survival and Growth Data -I Day M N 43.2% 0 P Q R 86.4% S T u v 100% w X 0 fD IC ID 10 IO *IC 10 l'() IO IO IO 10 I 1 10 IO 10 10 ID tO ID to ro to 10 .10 I 2 3

                                                                      /0     /{)  I0     /0        I0      I0     (0     10 10         I'{)   10
                                                                /fj   /Q      /Q   I()   IQ         fO IO         IC     ID IO         IQ      IO

-I 4 10 IO IO IC I() IC IC ID IO 10 lO to 5 10 10 10 /0 fD t() t() ID 10 tO 10 t() I 6 10 10 to 10 10 10 10 LO I() ID fO tO I A= Pan weight (mg) 7 I() IO lC 10 10 fQ (Q tO ft:J IQ 10 IO Tray color code:: --:-'l""-'"'""M...._.....,B,..w..,.t.'-- I Analyst: --~~1\A.l:..!l"*¥----- Date: _ ___,o...,;,._*..,'>>r.:..*.u.\l_ __ B =Pan+ Larvae weight (mg) -I Analyst: Date: 05 * *f*ll M ltf C =Larvae weight (mg) = B- A I Hand calculated()} Analyst: ..-- Weight per Initial number oflarvae (mg)

     = C /Initial number of larvae I    Hand calculated.

Analyst: c)1 v

                               /

I Average weight per Percent reduction initial number of from control (%) o.t1s 0.'"1 '\ \ o.1'lo larvae(me:) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, I lg = unusually large, d&r = decanted and returned, w = wounded. Comments: I -I I Page 33 of 100 SOP AT20- Exhibit AT20.3, revision 04-01-09

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EnvironmentiiTesting Solutions, Inc. Page 4 of6 Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant, OutfalllOl, Non-treated Date: OS*U2. \\ Day 0 lb 10 I D. 1 ID to tO /0 I 3 2 10 ID /0 ro /D . lO -I 4

                                                      /0       to   lD 5
                                                      /D     /0 I                                6 10      /0 7

I A= Pan weight (mg) It) (~ ro Tray color code:: -..,..Jo<L~,Gt~IH~t;~Lt.l~\- I Analyst: _ _ _..1!1\'+A\ltu...P_ _ __ Date: --~O<..:;?;a..:*-"'!)4:..1-!.1.... 8 = Pan + Larvae weight (mg) 1 _ _ __ Analyst: M. tte I Date: pc;. 14:*\1 C = Larvae weight (mg) = 8- A l I Hand calculated. Analyst: d-..__ Weight per initial number of larvae (mg)

     =C I   lnitial number of larvae I    Hand calculated.

Analyst: cJ _* J I\ ()' I\~ I Average weight per Percent reduction initial number of from co ntrol(%) 0 .'1 iD larvae Comment codes: c -= clear, d -= dead, fg = fungus, k = killed, m =missing, sk =sick, sm = unusually small, I lg = unusually large, d&r = decanted and returned, w = wounded. Comments: I -I I* Page 34 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

TVA I Sequoyah Nuclear Plant~ Outfall lOt Non-treated May 03-10,2011

**                                                                                          Pimephales promdas Ch ronk Wbole Emuent Toxicity Test EPA-821-R-02-013, Method 1000.0
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lluiltlll"'i'f-al (%) (-c) c- . or yaftdioe "*-...... PucuJ rwt.diM , , . . malnll (%' f%) A 10 10 14.&5 21.89 7.04 0.704 0704 B 10 10 14.o6 21.62 7.s6 0.756 0756 C....lnld 0 .770 6.6 IOCI.O 0.770 6.6 Nor appljcahle c 10 10 1434 22.3) 7.99 0.799 0 .199 D 10 10 13. 19 21.)8 8. 19 0.819 0819 E 10 10 13.36 21.85 8.49 0.849 0849 F 10 10 13.89 22.78 8.89 0.859 0.889 ltl.S% 0.851 70 IOCI.O lUlSI 7.0 -111.6 G 10 10 13.81 2279 8.98 0.898 0898 H 10 10 12.55 20.23 7.68 0.768 0768 I 10 9 14.01 20.60 6.59 0.7)2 0.659 J 10 10 12.85 2171 886 0.886 0.886 21.6% 0..853 9.6 !n.S o..sJ.4 14.1 -&.4 K 10 10 11.91 2l.04 9.1) 0.913 0.913 L 10 10 14.49 23.28 879 0.819 0.819 lll 10 10 1}.54 21.15 761 0.761 0.761 N 10 10 13.92 22.36 8.44 0.844 0.844 O..S25 -7.2 43.2% OJI2S S.2 100.0 S.l 0 10 10 1}.23 21.64 841 0.841 0 841 p 10 10 II 74 20.27 I.Sl 0.853 0.&53 10 10 1}_8) 22.21 845 0.84S 0.84S R 10 10 1}.79 21.84 ~~ 0.~ 0.~ 86.4% 0.791 6.S 100.0 0.791 6..S -U s 10 10 15.00 22.16 7.16 0.716 0.716 T 10 10 14. 76 22.7) 7'17 0 .7'17 0.7'17 u 10 10 B .63 21.64 8 01 0 .801 0.801 100% v 10 10 13.44 20.40 6.96 0696 0790 88 0696 100.0 0.798 8.1 -2..7 w 10 10 13.49 22.12 863 0.863 0863 X 10 10 12..97 2097 8.00 0.800 0800 y 10 10 14.J5 23.28 8.93 0.893 0.893

7. 10 10 ll.JI 21.32 7.94 0.794 94 0.794 110'% l obike 0..&51 n..5 0.7110 12.2 -1.4 AA 10 10 14.45 22.19 7.74 0..774 0774 BB 10 7 12.24 18.84 660 0943 0.660 Oulf.di!OI; MSO
  • Mintmum SigrufiC8r11 Doff.....,..,

Dcuoadt"s MSD YAhoo: 0.1196 PMSD= Pm:eot Miaimum Sig<Jiiicanl Oiffc:m><e PlllSD: 15..5 PMSO IS

  • meosureofle:sl pRCUIOIL 1be PMSD is lheminimum pcn:at1 differatce -lhecontrcl and tr_,_ char C3l be dedsal stab51icallysipif-.atn a wboleeffiuati iOJOCIIy leSt.

....a..: o..,adt's i\lSD value; 0.1052 U...-u PMSO bound clelammed by USEPA (I Olb - u l e )

  • 12%.

PMSD: IJ.7 l ' - PMS.O bowxl ~by US.EPA (901h pm:cntile)

  • 30%.

Low..- and upper PMSO boundswcrcdcu::nuincd liom lbe IOlb and~ pacentile, ~- ofPMSD d.Ufrom EPA's WET lo~etlaboralocyVariebilitySIO>dy (USEPA. 2001a; USEPA. 200lb). Ftle: ...,101_050311dala.ldsx Page 35 of 100

                                                                                                                                                                                                                                                                --o::-

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May 03-10, 2011 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/3/2011 TestiD: PpFRCR SampleiD: TVA I Sequoyah Nuclear Plant, Outfall 101 End Date: 5110/2011 LabiD: ETS-Envir. Testing SoL Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% 2 3 4 0-Control 0.7040 0.7560 0.7990 0.8190 10.8 0.8490 0.8890 0.8980 0.7680 21.6 0.6590 0.8860 0.9130 0.8790 43.2 0.7610 0.8440 0.8410 0.8530 86.4 0.8450 0.8050 0.7160 0.7970 100 0.8010 0.6960 0.8630 0.8000 Intake 0.8930 0.7940 0.7740 0.6600 Transform: Untransformed 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean 0-Control 0.7695 LOOOO 0.7695 0.7040 0.8190 6.623 4 0.8199 LOOOO 10.8 0.8510 Ll059 0.8510 0.7680 0.8980 6.967 4 -1.643 2.410 0.1196 0.8199 LOOOO 21.6 0.8343 1.0841 0.8343 0.6590 0.9130 14.114 4 -1.305 2.410 0.1196 . 0.8199 LOOOO 43.2 0.8248 1.0718 0.8248 0.7610 0.8530 5.190 4 -1.114 2.410 0.1196 0.8199 LOOOO 86.4 0.7908 1.0276 0.7908 0.7160 0.8450 6.839 4 -0.428 2.410 0.1196 0.7908 0.9645 100 0.7900 1.0266 0.7900 0.6960 0.8630 8.766 4 -0.413 2.410 0.1196 0.7900 0.9636 Intake 0.7803 1.0140 0.7803 0.6600 0.8930 12.249 4 Auxili!!!I Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p >0.01) 0.88409168 0.884 -1.217221232 1.2704004 Bartlett's Test indicates ~ual variances (!;! = 0.56) 3.9158771 15.0863171 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSQ2 MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.11955186 0.15536303 0.00391794 0.00492163 0.566574454 5, 18 Treatments vs 0-Control Linear Interpolation {200 Resamples) Point  % so 95%CL{Exp) Skew IC05 >100 ICIO >100 ICI5 >100 IC20 >100

                                           >tool IC40                                     >100 IC50                                     >100 File: sqnl01_0503lldata.xlsx Entered by: J. Sumner Page 36 of 100                                                                                                                                                                                            Reviewed by:---+--
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.,) Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 513120 I I TestiD: PpFRCR Sample ID: TVA I Sequoyah Nuclear Plant, Outfall I 0 I - intake End Date: 5/ 1012011 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimepbales promelas Comments: Non-treated Cone-% I 2 3 4 D-Control 0.7040 0.7560 0.7990 0.8190 10.8 0.8490 0.8890 0.8980 0.7680 21.6 0.6590 0.8860 0.9130 0.8790 43.2 0.7610 0.8440 0.8410 0.8530 86.4 0.8450 0.8050 0.7160 0.7970 100 0.8010 0.6960 0.8630 0.8000 Intake 0.8930 0.7940 0.7740 0.6600 Transform: Uotransfonned  !-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.7695 1.0000 0.7695 0.7040 0.8190 6.623 4 10..8 0.8510 1.1059 0.8510 0.7680 0.8980 6.967 4 21.6 0.8343 1.0841 0.8343 0.6590 0.9130 14.114 4 43.2 0.8248 1.0718 0.8248 0.7610 0.8530 5.190 4 86.4 0.7908 1.0276 0.7908 0.7160 0.8450 6.839 4 100 0.7900 1.0266 0.7900 0.6960 0.8630 8.766 4 Lntake 0.7803 1.0140 0.7803 0.6600 0.8930 12.249 4 -0.199 1.943 0.1052 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.0 I) 0.98316872 0.749 -0.2349007 0.4704176 F-Test indicates equal variances (p = 0.33) 3.51607203 47.4683456 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates oo significant differences 0.10523383 0.13675612 0.00023113 0.00586562 0.84920698 1, 6 T reatments vs D-Cootrol File: sqn 10 1_ 050311 data.x.Jsx Entered by: J. Sumner Page 37 of 100 Reviewed by: ~

TVA I Sequoyah Nuclear Plant, Outfall 101- Non-treated May 03-10, 2011 Pimephales promelas Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Cootrol 10.8% 43.2% 86.4 % 100~. 100% lota kr File: sqn101_050311 chem.xls Page 38 of 100 Entered by: S.drns Reviewed by:

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I Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant, Outfall lOt, Non-treated Date: OS* CS!>* \\

II I I CONTROL Non-treated I I 10.8% I 21.6% I I 43.2% I 86.4% I I 100% I I I 100% Intake -I I Page 39 of 100 SOP AT20- Exhibit AT20.3, revision 04-0 1-09

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Page 1 of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: Tennessee Valley Authority County: Hamilton Facility: Seguoyah Nuclear Plant Outfall: 101 NPDES #: TN0026450 Project#: __,_;o...;:;~....l _____ Dilution preparation information: ' Comments: Dilution prep (%) 10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080 2160 2500 I Diluent volume (mL) 2230 1960 1420 340 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism source information: Test information: ~I Organism age: Date and times organisms were born

                                                           < 24-hours old 05-&l\ 6,\<<a TO \'1.()1:)

Randomizing template color: Incubator number and shelf 6-ftLb between: location: '2.~2. I Culture board: ~'4*1.t.*\\ ~ Replicate number: 1 1 2 1 3 4jSj6j7jBj9 10 YWTbatch: O'l*1.\*l\ Culture board cup number: q I~ I, 1\ I \?;.I tl.\ I 'OS II& I'll lS Transfer vessel information: pH- I* 00 S.U. Temperature- "J..t.l.411 oc Selenastrum batch: o't*t\-1\ I Averaae transfer volume (mL): I 0*-~*SA Daily renewal information: I Day Date Test initiation and feeding, renewal and feeding, or termination time MHSW batch used Analyst 0 I 2 I 3 4 I 5 6 7 I Control information: Summary of test endpoints: I % of Male Adults:

  %Adults having 3ra Broods:

Control-I QT. Control-2 ()1. Acceptance criteria S:20% 7-day LC50 '71001. 1001 IDol.  :<:80% NOEC tcc7. I % Mortality: Mean Offspring/Female:

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                                                                                              <40.0%

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-I I Page 41 of 100 SOP ATll - Exhibit AT 11.2, revision 04-01-09

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il Species: Ceriodaohnia dubia l Client: TVA I Seguovab Nuclear Plant. Outfall lOt CONTROL-I Survival and Reproduction Data Date: .......zo-"!-;~*<Sk~..:..l\....___ __ II Day 1 z 3 4 Replicate number 5 6 7 8 9 10 a aL a a .I l Young produced l} ('\ 0 0 0 0 Adult mortality L- \...... L L \...... L... L \...... 1 '- 2 Young produced (')* 0. 0 D 0 0 D 0 0 0 Adult mortality \.... L- '- \.... \.._ L. ~ \.... I 3 Young produced Adult mortality c L... c

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Note. Adult mot1B11ty (L = hve, D =dead), SB =split brood (Single brood spht between two days), CO a cany over (of!Spnng earned over wtth edult dunng tr&IISfer). Concentration:

                                                                                                                   % Mortality:                                        I        07.

I CONC* 10.8% Mean Offspring/Female: Survival and Reproduction Data I '2.'\.1 Replicate number I Day 1 Young produced 1 () 2 () 3 0* 4 0 0 s 6 0 7' 0 8 0 0 9 10 () L \.._ \.._ Adult mortality L.. \..... \._

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                                                                   '5
                                                                     \...        L.
                                                                                   "5          ~
                                                                                                '-        L
                                                                                                            "\
                                                                                                                         \....

s L

                                                                                                                                      '1
                                                                                                                                                     \,_
                                                                                                                                                     '-\
                                                                                                                                                                   \......
                                                                                                                                                                    '1 L.

t..'* Adult mortality \.._

                                                                    '-              L          L..         '-         L             L             '-               L..            '--

I s Young produced Adult mortality

                                                       \~
                                                         \.....

ro I~

                                                                                 \....

l"l. L '- 10 L 1"2.. L ll. tO

                                                                                                                                                  \..._          \...

ll 1\ L 6 Young produced 0 n 0 0 (\ 6 () (') 0 0 I 7 Adult mortality Young produced n L. L

                                                                 \S L-
                                                                                 \\c,          \\o L
                                                                                                            'o L...        L
                                                                                                                        \\o L..         L l'-\

L l5 \S L Total young produced

                                                     ~~          ~()            ~'-\           ~l.         1.1        ~3             '"""

3() "Z.i 3() ~L... I Final Adult Mortality \ L '- L L L L L.. '-- "- Note: Adult mortality (L ~live, D m deed), SB -split brood (single brood split between rwo days), CO= can over (of!Spring carried over with adult during transfer . Concentration: -I  % Mortality: Mean Offspring/Female: 07. 3o.'l

                                                                                                                   %Reduction from Control-!:                             *C.(,C7o I Page 42 of 100                                                                                       SOP ATll - Exhibit ATll.2, revision 04-01-09
            ~~Tc; Page 3 of7
            )!; j d
           .) &nvlnm:ntenUJTutlngSoM!ons.II'IC.

Species: Ceriodaohnia dubia Client: TVA I Sequoyah Nuclear Plant. Outfall lOt Date: ___,Oi...,*,_*<B""""'"'*'""\~\_ _ __ coNe* 21.6% Survival and Reproduction Data Replicate number Day I :z 3 4 s 6 7 8 1 Young produced (\ 0 C'J 0 0 _0_ 0 0 0 ' a. 10 Adult mortality L L- '- L.. L L. L L... L L...

z Young produced D 0 D c (") D 0 0 0 ()

Adult mortality L- \. L. L L. '-- '-- L. '-- L... 3 Young produced D 0 D c b .() 0 c 0 0 Adult mortality

                                                             '-          L.          \....         '-             L-        L             '--           '-                  '- '--

4 Young produced "-\ ~ '-\ . '-\ 0 '-\. '-( '-\. '4 "( Adult mortality L. '- '-- '- '-- '- '- \..:... \.._.. I s Young produced Adult mortality

                                                           \"t L
                                                                        \0 L
                                                                                    \"L L..

L l1...

                                                                                                             \..__

l'l.. L

                                                                                                                                        \'1
                                                                                                                                        '-           L l"L                I\

1\ 6 Young produced 0 0 c D 0 0 D 0 0 0 -i I Adult mortality

                                                             \......    \_.
                                                                                      '-              L-        L             L.         L            '-                  L          \.._

i 7

                                                                                    '~                        "~         \~                            \~                             \~

Young produced tS - Total young produced I Final Adult Mortality

                                                         ~'!,
                                                                     ,!, '1.       3\
                                                                                   ~
                                                                                                 ~\o
                                                                                                              '2.."\

3!

!.\

L.... 3\ Note: Adult mortality (L = rwe. D =dead), SB =split brood (single brood spOt between two days), CO

  • can over offSpring carried over witb aduh during transfer .

3\

                                                                                                                                                                                     ~3
                                                                                                                                                                                        \.,..._

Concentration: I  % Mortality: Mean Offspring/Female: 07.

                                                                                                                                                                                 ~l.Dt
                                                                                                                      % Reduction from Control-1:                              -,,**\"1.

I CONC: 43.2% Survival and Reproduction Data Replicate number Day 1 :z 3 5 7 8 0' 4 6 10 I 1 Young produced Adult mortality 0 L-0 L. L 0 L 0 L () L 0 0 L L 0 L 0 L.

z Young produced 0 C) c D 0 0 D a 0 0 I 3 Adult mortality Young produced c

0 a

                                                                                     \._         \........

0 0 L L 0 L. c 0 0 0 Adult mortality L. L- L.. \...... L- '-- L L. '- L. I 4 Young produced Adult mortality

                                                            ~
                                                          \........
                                                                       '-\
                                                                        \...

s L. "'

                                                                                                 \......
                                                                                                               ~

L.

                                                                                                                         '-1 L
                                                                                                                                        ~

L

                                                                                                                                                      '-\

L-

                                                                                                                                                                           '"l L        '-
                                                                                                                                                                                        "t s                                           n                                                                 '1..                                                      l"L
                                                                                                                                         *~            *~

Young produced \'l,. t\ 11. *3 I 6 Adult mortality Young produced L 0 L c\._ C'1 L 0 0 L L 0

                                                                                                                                       ~

0 0 L. 0

                                                                                                                                                                            \....

0 L_ Adult mortality L L L \._ L L L L l. I 7 Young produced Total young produced l~ 3~

                                                                     &(,.
                                                                      ~'2..       ~
                                                                                   \~           \~
                                                                                               ~()

l4\

                                                                                                              ~5 "Z.O 3~
                                                                                                                                       \~
                                                                                                                                       ~s
                                                                                                                                                      \"'\

3"1

                                                                                                                                                                        \S al.
                                                                                                                                                                                     \Q
                                                                                                                                                                                    .!~
                                                                                                                                                                       \.......

I Final Adult Mortality L. L. '- L.... L- \....... '- L Note: Adult mortality (L e live, D a dead), SB split brood (single brood split between two days), CO e can over offSpring carried over wit~ adult during transfer , B Concentration:

                                                                                                                                                                                    \........
                                                                                                                     % Mortality:                                                  07.

-I Mean Offspring/Female:

                                                                                                                     %Reduction from Control- I:

3'?l.l

                                                                                                                                                                               -r!..t1..

I Page 43 of 100 SOP ATil - Exhibit ATll.2, revision 04-01-09

I' I Page4 of7 I Species: Cerlodaphnia dub/a I Client: TVA I Seguoyah Nuclear Plant. OutfalllOl coNe*. 86.4% Survival and Reproduction Data Date: O~*&l\ lI Reolicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 0 0 () 0 0 L c 0 Q Adult mortality '-- '- \..... L L.. L L '- L II 2 Young produced Adult mortality 0

                                                          \.....

0

                                                                      . '- '-- L.

0 0 0 0 0

                                                                                                                                                \......

0

                                                                                                                                                              \.....

c L. '- 0 l 3 Young produced c D D () 0 0 _C) 0 c a I L- '-* '- Adult mortality L *I..... \.._ '-- L '-- I 4 Young produced s ~ s s '-\ ~ "- --"i \o s Adult mortality L- '-- '-- '-- L. L.... L L.

                                                                         '-         \....

II j 5 Young produced Adult mortality

                                                          \~

L.

                                                                        \"l L..
                                                                                     \1..

L

                                                                                                    \4
                                                                                                   \....
                                                                                                                 \l.

L

                                                                                                                                \\

L. \._.

                                                                                                                                               \\             \L.
                                                                                                                                                              \_               \.....
                                                                                                                                                                                            '~

L 6 Young produced r'l

                                                           -L D            ()             0             c               ()              D             b               0             D JI Adult mortality                                L..        '-            '- '--                         L              L            '--             L-           L 7          Young produced                 \lo           1~           \\D
                                                                                                  -         '"Z.O           '1."1..          \'\          \C\              \S            \i Total young produced
                                                        ~             ~s           ~~             ~s           ~lo              31             ~~             35             ~0            35 I      Final Adult Mortality D

L. \._ \........ \...... \...... L.. '-- '-- Note: Adult mortality (L = live, D dead), SB - split brood (single brood split between two clays), CO = car over offSpring earned over with eduh duriog translet' . Concentration:

                                                                                                                                                                              '--            L..

I  % Mortality: Mean Offspring/Female:

                                                                                                                          % Reduction from Control-I:

07. 3'-1.'\

                                                                                                                                                                                   - l1.'S7.

I CONC* Day 100% Survival and Reproduction Data Replicate number 1 2 3 4 5 6 7 8 9 10 I 1 Young produced 0 0 ~ ~ a 0 0 0 0 c Adult mortality L L L L l J. L L L L 2 Young produced 0 0 0 a 0 0 0 0 _0. D I 3 Adult mortality Young produced L ('"') 0 L L. 0 D L (} L...

                                                                                                                                ~

L c L.. '-- (j 0 L

                                                                                                                                                                                          ~

L Adult mortality

                                                          "'--           \_         L.             L             ~             L                 '-           L.             L.          '-

I 4 Young produced s 5 la s s '-\ ~ (. 4 s Adult mortality

                                                        '---            \....         '--           L           '-              \....           L             L              \......        L.

5 Young produced l"'L. l1.. \~

                                                                                                   '~           11.             ' -'            n ..            l"t            rt..         I'Z...

I 6 Adult mortality Young produced () L D L (\ 0

                                                                                                   \._           L

() 0 L. L 0 c L L 0 L 6 Adult mortality L L L L L L L L 1 l I 7 Young produced Total young produced

                                                        '~

3k

                                                                     '~

3<o

                                                                                  \\
                                                                                   ~~...
                                                                                                 \~

a\

                                                                                                              \&

a~ La

                                                                                                                               ~1
                                                                                                                                              \i
                                                                                                                                              ~~             ~~
                                                                                                                                                                          "1..0
                                                                                                                                                                            ~   .

l(\ 3'- Final Adult Mortality L. L.. L L L L. L L.. L. '--- I Note: Adult mortality (L ~ live, D dead), SB split brood (single brood split between two clays), CO - ca D over oll's!>ri~ carried Concentration: over with adult durin_A transfetl,

                                                                                                                          %Mortality:                                                 07.

-I Mean Offspring/Female:

                                                                                                                          % Reduction from Control- I:
                                                                                                                                                                                   .';!.., 1.[
                                                                                                                                                                                  -n.c..7~

I Page 44 of 100 SOP AT l I - Exhibit AT I 1.2, revision 04-0 I-09

! :;;;:;qz.;.F"'" l~I Page 5 of7 jI II Species: Ceriodaohnia dubia Client: TVA I Seguoyah Nuclear Plant, OutfalllOl CONTROL-2 Survival and Reproduction Data Date: OS-0* \\ I Day l 2 3 4 Replicate number 5 6 7 8 9 10 l Young produced a c a 0 a c c 0 c Q J I '- '-- '- Adult mortality "- \.._... L '- '- \...... \.._ 2 Young produced () i> 0 D a 0 0 0 0 0 Adul~ mortality \..... L- \..... L- L.... L- L.... \...... ~ \,_ I 3 Young produced Adult mortality

                                                                       ~

L.. 0 L. 0

                                                                                                \.....:.

D D t:> L 0 () L c

                                                                                                                                                                                 \.....

0 L... 4 Young produced '-\ ~ ~ ~ ~ ~ '-\ ~ "\ I s Adult mortality Young produced L...

                                                                     \"'l..

tO

                                                                                                  \.....
                                                                                               \"1.

L

                                                                                                             \ \
                                                                                                                          \\
                                                                                                                                     \\
                                                                                                                                                   \......

l\

                                                                                                                                                                 \1.
                                                                                                                                                                               \'L 10 Adult mortality                   L.             \..... L             L         \.,_
                                                                                                                                       '- '--                       L            L         L.

I 6 Young produced Adult mortality f) L. 0 c 0 D (J L.- a 0 0 0

                                                                                     \......                 '--
                                                                                                                                                               \,'--
                                                                                                \......                    L.                                                   \.._          L 7          Young produced                   \~            \~          \\              l\o        \S        L~            \$                            \S          \S I                   Total young produced
                                                                   '2.'\

L-

                                                                                 'Z.t:.        ~~            :=o         "2.'\      1.1           ~b            3'2.            3\           2.~

Final Adult Mortality '-- \._.

                                                                                                             ~
                                                                                                                \._..
                                                                                                                         '--        L               L-           \..._
                                                                                                                                                                               '-             ~

X for 3ra Broods X )( )(.. '>L X.. ~ 'X- X: ~ -I Note. Adult mortality (La live. D =dead), SB ~ spht brood (single brood spilt between two days), CO= carry over (ofl'spnng earned over With adult dunng tnlnslior). Concentration:

                                                                                                                                  % Mortality:                                     I     07.

I CONC: 100% Intake Mean Offspring/Female: Survival and Reproduction Data I "2,q~, Replicate number I Day 1 Young produced 1 0 0 2 0 3 C\ 4 a s () 6 a 7 8 0 Q 9 () 10 Adult mortality L L L L L L l L \. '--- I 2 Young produced Adult mortality 0

                                                                       \.....

0

                                                                                      \.....

0 L 0 L L D L. {} L 0 L 0 c _Q L. 3 Young produced a () c a Q c 0 0 0 0 I 4 Adult mortality Young proauced l

                                                                      \.o L          L..

s L..

                                                                                                             "'\

L. 5 ~ L L. 4

                                                                                                                                                                \.....
                                                                                                                                                                 ~
                                                                                                                                                                                \.....
                                                                                                                                                                              \.(

L. lf Adult mortality '-- --

                                                                                   ""        L.          \......       '--         '--           '-             '--             '-           '--

I s Young produced Adult mortality 1~ L n.

                                                                                   \--       L*~             "  L n.
                                                                                                                           \....      L ll..         ID L          *~

L *~ L l II 6 Young produced 0 0 0 0 () 0 () (\ 0 0 I 7 Adult mortality Young produced L

                                                                    \~         1.\

L '- l\o (0 L L "l.D L t& \& L L

                                                                                                                                                              \'\

L "2.\ L 1.\ Total young produced

                                                                   ~1           31           3'S           ~~           ~1          0~             ~1-         31..            3~           3\o I                  Final Adult Mortality                             \._            L-        \..            L          L         L.           L.             \.,_             L Note: Adult mortality (L- live. D a dead), SB -split brood (single brood split between two days), COs can over ofi'SPrinq carried over with adult durinJI u6nsferl.

L.. Concentration: I -I  % Mortality: Mean Offspring/Female: 07. 3S.!

                                                                                                                                  % Reduction from Control-2:                         -"Zo.s7.

I Page 45 of 100 SOP ATil - Exhibit ATll.2, revision 04-01-09

                                                                                                                                                        .. I    *1***1.o>,"Z1*U'..-,~t.:..tw:...,.:;;.,:...<...-~IL<*Io.;:O!.:U..I_,,.#O-"'""-'-.....,_..*~*,

TVA I Sequoyah Nuclear Plant, OutfalllOl -Non-treated May 03-10,2011

     *.ETS t..:J . ***ii'i?:c;:      :,.     .....,:....                           Verification of Ceriodllphnill Reproduction Totals
    . ) Envii'Dilft>GfltaiTestlng5olullans,lnc.

Control-I 86.4% Re licate number Renllcate number Day Total Day Total 1 l 3 4 5 6 7 8 9 10 I l 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 3 4 4 5 4 4 4 4 4 40 4 5 4 s 5 4 4 6 4 6 5 48 s 10 11 11 12 11 12 12 10 12 12 113 5 13 12 12 14 12 II II 12 12 12 121 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 14 14 16 15 15 15 14 15 13 13 144 7 16 19 16 16 20 22 19 19 15 18 180 Total 28 28 31 31 31 31 30 29 29 29 :m_ Total __ 34 35 ~ 35 36 37 36 35 33 35 349 I 10.8%

               ---                                                                                         100%

Replicate number Day R plicate number Day Total Total 1 2 3 4 s 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 3 5 5 4 4 5 4 4 4 6 44 4 5 5 6 5 s 4 6 6 4 5 51 s 13 10 13 12 10 12 12 10 II II 114 s 14 12 13 13 12 14 12 12 12 12 126 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 15 16 16 13 16 14 14 15 15 lSI 7 19 19 17 19 18 20 18 18 20 19 187 Total 33 30 34 32 27 33 30 28 30 32 309 Total 38 36 36 37 35 38 36 36 36 36 364 I 21.6%

                   ---     r~

Control-2 Reolicate number Day ReDileate number Day Total Total I 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 s 4 4 3 4 4 4 4 4 40 4 4 3 5 3 3 3 4 3 4 4 36 s 12 10 12 13 12 12 12 12 II II 117 5 12 10 12 II 11 II II 12 12 10 112 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 17 IS 19 14 16 IS 15 16 18 162 7 13 13 17 16 IS 13 15 17 15 15 149 Total 33 32 31 36 29 32 31 31 31 33 319 - Total 29 26 34 30 29 27 30 32 31 29 297 43.2%

                - - . -                                                                                     tOO% Intake Re  licate number                                                           Re licate number Day                                                                  Total                Day                                                                 Total 1       2        3  4       5      6   7  8    9   10                                  I  2   3  4     5      6   7  8     9      10 1         0      0         0  0       0      0   0  0    0    0      0                   I      0   0   0  0     0      0   0  0     0       0                 0 2         0      0         0  0       0      0   0  0    0    0      0                  2       0   0   0  0     0      0   0  0     0       0                 0 3         0       0        0  0       0      0   0  0    0    0      0                  3       0   0   0  0      0     0   0  0     0       0                 0 4         6       4        5  4       4      4   3  4    4    4     42                   4      6   4   5  4      5     5   4  4     4       4                45 s         II     12       14  II      12    12  13  13  13   12    123  I                s      13 12  14 11     12    12  10 13    13      11               121 6         0       0        0  0       0      0   0  0    0    0      0                   6      0   0   0  0      0     0   0  0     0       0                 0 7         19     16       15  15     19     20  19  17  IS   18    173                   7     18  21  16 20    20     18  18 19    21      21               192 Total        36     32       34  30      35     36 35  34   32   34    338  I             Total    37  37  35 ~ 37         35 31  36    38     36                358 Page 46 of 100 File: sqn101_050311data.xlsx Entered by: J.

Reviewed by. Jner

                                                                                                                                                                   -   -*L- -       ,,..., _ _.,..._.~ - ~~..._.. __.,

TVA I Sequoyah Nuclear Plan~ Outfall lOt Non-treated May 03-10, 2011

  • Ceriodaplmia dubia Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1002.0
~

\.....) Quality Control ~ Environmental Testing Solutions, Inc. Verification of Data Entry, Calculations, and Statistical Analyses Project numbe.r: 7068 Concentration Re_11licate aumbe.r Survival Average reproductio.n Cocllic:ioat of P HUDI red*ctloto 11om (~*) ( */e) (offs:priaglremate) variatioa (%) -...a(%1 I 2 3 4 5 6 7 8 9 JO Control - J 28 28 31 31 31 31 30 29 29 29 100 29.7 4.2 Not applicable 10.8~. 33 30 34 32 27 33 30 28 30 32 100 30.9 7.4 -4.0 21.6~. 33 32 31 36 29 32 31 31 31 33 100 31.9 5.8 -7.4 43.2~. 36 32 34 30 35 36 35 34 32 34 100 33.8 5.7 -13.8 86.4*/. 34 35 33 35 36 37 36 35 33 35 tOO 34.9 3.7

  • 17.5 100% 38 36 36 37 35 38 36 36 36 36 100 36.4 2.7 -22.6 Control-2 29 26 34 30 29 27 30 32 31 29 100 29.7 7.8 Not applicable J OO~o lata.kc 37 37 35 35 37 35 32 36 38 36 100 35.8 4.7 -20.5 Outran JOI : MSD = Minimwn Significant Difference OUBnett's 1\tSD value: 1.698 PMSD = Percent Minimum Significant Difference PMSD: 5.7 PMSD is a measure of test precisiOIL The PMSD is the minimum percent difference bern-een the oontrol and treatment that can be declared statistically significant in a whole effiuentto.xicity tesL latakt:

OUBnett's MSD value: 1.569 l.ov.'ef" PMSD bound determined by USEPA (Ia- percentile) = IJ'Yo. PMSO: Upper PMSD bound detennined by US EPA(~ percentile)= 47%. Lower and upper PMSD bounds were determined from the I Oth and 90th percentile, respectively, of PMSD data from EPA's WET lnterlaboratOJy Variability Study (USEPA, 2001a; USEPA. 2001b). USEPA. 2000. Understanding and Accounting for Me!hod Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-()().()()3. US Environmental Protection Agency, Cincinnati, OH. US EPA. 200la. 200 Ib. Final Repon: llltcrlaboratoty Variability Study of EPA Short-term Otronic and Acute Whole Effluent Toxicity Test Methods. Volumes I and 2-Appendix. EPA-821-B-0 1-004 and EPA-821-B-0 1..005. US Environmental Protection Agency, Cincinnati, OH. File: sqn101_050311data.xlsx Table populated from associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheeL Page 47 of 100 Spreadsheet entered by: J. Sud\r Reviewed by:

I,,,._,_., TVA I Sequoyah Nuclear Plant, Outfa11101 Non-treated

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Environmental Testing Solutions, Inc. May 03-10,2011 Statistical Analyses Ceriodaphnia Swvival and Reproduction Test-Reproduction Start Date: 5/3/2011 TestlD: CdFRCR Sample 10: - TVA I Sequoyah Nuclear Plant, Outfall 101 End Date: 5/10/2011 LablD: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Conunents: Cone-% 2 3 4 5 6 7 8 9 10 Control- I 28.000 28.000 31.000 31.000 31.000 31.000 30.000 29.000 29.000 29.000 Control-2 29.000 26.000 34.000 30.000 29.000 27.000 30.000 32.000 31.000 29.000 10.8 33.000 30.000 34.000 32.000 27.000 33.000 30.000 28.000 30.000 32.000 21.6 33.000 32.000 31.000 36.000 29.000 32.000 31.000 31.000 31.000 33.000 43.2 36.000 32.000 34.000 30.000 35.000 36.000 35.000 34.000 32.000 34.000 86.4 34.000 35.000 33.000 35.000 36.000 37.000 36.000 35.000 33.000 35.000 100 38.000 36.000 36.000 37.000 35.000 38.000 36.000 36.000 36.000 36.000 Intake 37.000 37.000 35.000 35.000 37.000 35.000 32.000 36.000 38.000 36.000 Transform: Untransformed  !-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean Control- I 29.700 1.0000 29.700 28.000 31.000 4.214 10

  • 32.933 1.0000 Control-2 29.700 1.0000 29.700 26.000 34.000 7.784 10 10.8 30.900 1.0404 30.900 27.000 34.000 7.388 10 -1.616 2.287 1.698 32.933 1.0000 21.6 31.900 1.0741 31.900 29.000 36.000 5.809 10 -2.963 2.287 1.698 32.933 1.0000 43.2 33.800 1.1380 33.800 30.000 36.000 5.717 10 -5.523 2.287 1.698 32.933 1.0000 86.4 34.900 1.1751 34.900 33.000 37.000 3.687 10 -7.005 2.287 1.698 32.933 1.0000 100 36.400 1.2256 36.400 35.000 38.000 2.654 10 -9.025 2.287 1.698 32.933 1.0000 Intake 35.800 1.2054 35.800 32.000 38.000 4.711 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.0 I) 0.829582155 1.035 -0.138685175 0.300894097 Bartlett's Test indicates equal variances (p = 0.13) 8.592660904 15.08631706 The control means are not significantly different (p = 1.00) 0 2.100923666 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dwmett's Test 100 >100 1.697549464 0.057156548 64.58666667 2.755555556 1.9E-12 5,54 Treatments vs Control- I Linear Interpolation (200 Resamples)

Point  % so 95%CL Skew ICOS >100 IC10 >100

  !CIS                                  >100 IC20                                 >100 IIC25                                    >tool IC40                                 >100 IC50                                 >100 File: sqnl01_05031ldata.xlsx Page 48 of 100                                                                                                                                                                                         Entered by: J. Sumner Reviewed by:     J{

o.ol --~ -"-"-'*****--~- ** :.r**--'L~--~-~~ TVA I Sequoyah Nuclear Plant, Outfall 101 -Intake Non-treated May 03-10, 2011 .ETS

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Environmental Testing Solutions, Inc. Statistical Analyses Ceriodaphnia Survival and Reprodu~ion Test-Reproduction Start Date: 5/31201 I Test iD: CdFRCR Sample ID: TV A I Sequoyah Nuclear Plant, Outfall I0 I - Intake End Date: 511012011 LablD: ETS-Envir. Testing Sol. Sample Type: DMR-Discbarge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Cone-% I 2 3 4 5 6 7 8 9 10 Control- I 28.000 28.000 31.000 31.000 31.000 31.000 30.000 29.000 29.000 29.000 Control-2 29.000 26.000 34.000 30.000 29.000 27.000 30.000 32.000 31.000 29.000 10.8 33.000 30.000 34.000 32.000 27.000 33.000 30.000 28.000 30.000 32.000 21.6 33.000 32.000 31.000 36.000 29.000 32.000 31.000 31.000 31.000 33.000 43.2 36.000 32.000 34.000 30.000 35.000 36.000 35.000 34.000 32.000 34.000 86.4 34.000 35.000 33.000 35.000 36.000 37.000 36.000 35.000 33.000 35.000 100 38.000 36.000 36.000 37.000 35.000 38.000 36.000 36.000 36.000 36.000 lntake 37.000 37.000 35.000 35.000 37.000 35.000 32.000 36.000 38.000 36.000 Transform: Untransformed 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control- I 29.700 1.0000 29.700 28.000 31.000 4 .214 10 Control-2 29.700 1.0000 29.700 26.000 34.000 7.784 10 10.8 30.900 1.0404 30.900 27.000 34.000 7.388 10 2 1.6 31.900 1.0741 31.900 29.000 36.000 5.809 10 43.2 33.800 I.I380 33.800 30.000 36.000 5.717 10 86.4 34.900 1.1751 34.900 33.000 37.000 3.687 10 100 36.400 1.2256 36.400 35.000 38.000 2.654 10 Intake 35.800 1.2054 35.800 32.000 38.000 4.711 10 -6.741 1.734 1.569 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.949571669 0.868 -O. t3n86t 5 0.558140392 F-Test indicates equal variances (p = 036) 1.87890625 6.54I085n The control means are not significantly different (p = 1.00) 0 2. 100923666 Hypolhesis Test ( 1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homosceda.stic t Test indicates no sigoiiiCllot diffenoces 1.569196635 0.052834904 186.05 4.094444444 2.6E-06 1, 18 Tnatmeots vs Cootrol-2 file: sqnl01_ 0503 1ldata.xlsx Page 49 of 100 Entered by: J. Sumner Reviewed by: J'\

I. TVA I Sequoyah Nuclear Plant, Outfall101 -Non-treated May 03-10, 2011

  • Ceriodaphnia dubia Chronic Whole Emuent Toxicity Test u

~ E s . Environmental Testing Solutions, Inc. Coneentration EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses 7068 Control 10.8% 22.6% 43.2% 86.4% 100% 100% Intake File: sqn101_050311chem.xls Page 50 of 100 Entered by: s;rns Reviewed by:

I Page 6 of7 I Species: Ceriodapltnia dubia Date: OS*<S'b*\\ I Client: TVA I Seguoyah Nuclear P lant, OutfalllOl I I I CONTROL I 10.8% I I 21.6% I 43.2% I I 86.4% I I tOO% ' 100% Intake Page 51 of 100 SOP AT II - Exhibit AT 11.2, revision 04-01-09

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Species: Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant, OutfalllOl I~ I Parameter I. CONTROL I; li 10.8% ll II 21.6% I* 43.2% I I I' 86.4% I I 100% f I 100% Intake Page 52 of 100 SOP ATil - Exhibit AT II .2, revision 04-0 1-09

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I Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas County:~ t-\N*W.:r"ON I Client: Tennessee Valley Authority Facility: Seguoyah Nuclear Plant NPDES #: ..*n~9829168t' ~ Ot>'2J.'-\'SO Outfall: 101 1o<..8

I Project #:

Dilution preparation information: Comments: Dilution prep (%) 10.8 21.6 43.2 86.4 100 Each concentration was UV -treated I Effluent volume (mL) Diluent volume (mL) 270 2230 540 1960 1080 1420 2160 340 2500 0 for 2 minutes to remove pathogenic Interferences. Total volume (mL) 2500 2500 2500 2500 2500 I Test O'f!anism information: Test information: Organism age: .,\ "\'i "-'oJJlf:. o~ Randomizing template: 6\.ve. -I Date and times organisms were born between: 05-0"t*\\ *'-00 Incubator number and shelf location:

                                                                                                                            .3&

Organism source: lttml'L e,:..~ ~ oc;-cn... l \ Artemia CHM number: c..Ht-\~ I Drying information for weight determination: Transfer bowl information: pH= "'\,CD& s.u. Date I Time in oven: 05*~0*\\ oc I Average transfer volume: Temperature.= "\.".'1....

c. \1\\.~

Initial oven temperature: loti'(. Date I Time out of oven: OS*\\* \\ Final oven temperature: Coc'<.. I Daily feeding and renewal information: Total drying time: 1.'l* ~01)..~ I Day Date Morning feeding Afternoon feeding Sample numbers used batch I 0 I 2 3 4 I 5 6 I 7 ll

 .I I       Page 53 of 100                                                                       SOP AT20- Exhibit AT20.3, revision 04-01-09
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Page 2 of6 I Species: Pimephales promelqs I Client: TVA I Seguoyab Nuclear Plant, OutfalllOl. UV-treated Date: OS*<Ai*t\

                                                              '                                           Survival and_~ Data I                                   Day A

l.:U~IKUL B c; Q E 1' 10.8%_ G H _[_ J 21.6% K L 0

                                                                      /I)      /C        /C       IO        10 *IC            /C)       ID       ID         IO 10 10 I                                       1 (Q      10         10 10             10        /0       /0        lO        10       rc      10     .10 2

I 3 10 10 ro ro 10 10 /0 IO 10 IO IO 10 to fQ 10 ft;, It) 10 IC IQ IO IC 10 10 I 4 It> 10 /0 'O ro IO /Q IO IO to IO (0 s to IO IO 1o 10 ID to /0 /Q /Q 10 10 I 6 to tO to /0 16 10 I() IO IO tO 10 IO 7 lo" ~~ IO 10 to I A = Pan weight (mg) IO 1o IO IC IO IO IO Tray color code:: ~~-rill tf.Ol I Analyst: Date: 0~*~*\l 8 =Pan+ Larvae weight (mg) rv'l 1~.1~ \Z,..~ \?>>.;~ I~.QIIl 1~.!1~ l~.z.-4 14.91 14-.(,& t4.'i>!. \;.\(, t;.\ol Analyst: Mltl [tt:4~ ltJ1.'1 [c..r. " t.t..t.'l lt.t.lt. !t..t*Sl lv:i-.lo ~~.\1.. lt13.G,4 ?,l,'f"' I Date: OS* I~* II Z.0-1" iu.JtJ I C =Larvae weight (mg) = ~-A Hand Analyst: AI-- ,*'"' C\,o& . ~~

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I Average weight per initial number of larvae(me) Percent reduction from control(%) o.R~\ o.l<-1 ~* .,.. o.~l."\ o.t7. Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, I lg = unusually large, d&r = decanted and returned, w = wounded. Comments: I I Page 54 of 100 SOP AT20- Exhibit AT20.3, revision 04-01-09 II

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~ I Species: Pimeohales oromelas I Client: TVA I Seguoyah Nuclear Plant, Outfa11101. UV-treated Survival and Growth Data I Day M N 43.2% 0 p Q R 86.4% s T u v 100% w X 0 (0

                                                              /()       ID IO           ID /D -10                             IC       10 IO             If;)       fO I                                  1
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I tO ((J 1\ \0 to tc tc lD ((J 10 4ll A= Pan weight (mg) Tray color code:: M!mfa,!!fl I Analyst: Date: frll\tC 0~*!2!!:*\~ B =Pan+ Larvae we~ht (mg) 14*1'- 14.4z, l~.t{., 14-?A \;.~~ It).~":> 14.2k t+.c.-1 lt-.;') 1\.1\C.. \lr.?lo l4-.~c; Analyst: M u.,, 'to.~ I Date: 02*1~*ll C = Larvae weight (mg) = B- A t.?>.SS t,z..ti[f U..1,~ t.'L.It. u.:u  !:L.'U z.z.."' r't.'la u.4? V...t.~ t.-t\ c:n. &.'4, ,.,~ t.So ,,,b ,,,1.\ l"l*C\' t.ll. ** Q1. c..C.b Q.t.fb I Hand calculated(!}._ Analyst: "'- Weight per initial number of larvae (mg) I

        = C I Initial number oflarvae
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I Average weight per Percent reduction initial number of larvae (me) from control(%) o.S~i *o.t7. o.,~i '(. 01. o:t&o c.. '1.7.. Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, I lg = unusually large, d&r = decanted and returned, w = wounded. Comments: I -I I Page 55 of 100 SOP AT20- Exhibit AT20.3, revision 04-01-09

Page 4 of6

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Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant., OutfalllOl, UV-treated i ~ I Day 0 I /0 IO 10 2 to tO f() to 3 I() ({) ID 4 IO IO 'O 5 ID 10 lO 6 7 A = Pan weight (mg) Tray color code:: M'/l:uh'\flr Analyst: M \!:C Date: oC:., -o4.\\ B =Pan + Larvae weight (mg) Analyst: MM Date: 012*l't* n C = Larvae weight (mg) = B - A Hand calculate~ Analyst: 61.,. Weight per initial number of larvae (mg)

     = C I Initial number of larvae Hand calculated.

Analyst: --<QI-~~-=----- Average weight per Percent reduction initial number of from control(%) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: Page 56 of 100 SOP AT20- Exhibit AT20.3, revision 04-01-09

TVA I Sequoyah Nuclear Plant, Outfall 101 UV-treated May 03-10, 2011 Pimephales pronrelas Chronk Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control (] Verification of Data Entry, Calculations, and Statistica l Analyses ~ Environmental Testing Solutions, Inc. c-.......SO. (%) ......... ....... bi4&l *UIIJtt fll l""lallle...a.:tartuTar A

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                        £             10                      10                1509               2410                 9.01                 0.901                                                               Q901 F             10                      10                1335               2 1 48               8. 13                0.8 13 4.4 0.813 10..1%                                                                                                                                                       0.1167                                                        100.0            0..167                   4.4                 -0 c             10                      10                IJ.24              2 1 91               8_67                 0.867                                                               0.867 H             10                      10                14.87              23.n                 s.ss                 0 .88S                                                              0.885 I            10                      10                14.68              23.64                8.96                 0.896                                                               0.896 J             10                      10                14.06              2 1.99               7.93                 0.793                                                                0.793                                                                          0,.1 21.6%                                                                                                                                                        0824                   6.J                                    10(1.0          G.8l4                     6.3 K             10                      10                13.16              20.95                7.79                 0.779                                                                0.779 L             10                      10                13. 14             21.43                8.29                 0.829                                                                0.829 M             10                      10                14.76              23 55                8.79                 0.879                                                                0.879 43.2%

N 0 10 10 10 10 14.42 13.76 22.94 22..23 8.52 8.47 0.852 0847 0.859 1.6 0..152 0.847 91.5 0.838 5.4 ..... p 10 9 14.39 22.12 7.73 0.859 o.m 0 10 10 1383 22.33 8.50 O.SSO 0.850 86..4 % R 10 9 15.23 2293 1.70 0.856 0819 4 .9 o.no 91.5 0.791 4.6 4.0 s 10 10 1426 22.00 7.74 O.TI4 OTI4 T 10 10 14.64 22.61 7.97 07'Y/ 0.'197 u 10 10 12.55 20.71 8.16 0 81 6 0.8 16 100% v 10 10 11.96 1998 8 02 0802 0803 139 0.802 91.5 0.780 10.4 6.2 w 10 10 14.80 2 1.40 6.60 0.660 0.660 X 10 9 1485 2325 8.40 0.93) 0.840 y 10 10 13.26 22.74 9.48 0.948 0 .948

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  • M mmun Sopoanl Difl'c<CDCC Douo -t'a MSD vatu: PMSD* Percent Mmunum s;p;r_,. DUI'cn:nce P MSD: 12..1 PMSD tS a III<:OSift of tesz precmon. Tbe PMSD os ohe minimum perc:eot dif!'..-..-.......,_, ohe OCIIIIfOI and tn:a<men1 lhat can be clcdarcd ............Oy sognif~Cant "' a ¥oilole cftlu<na ooxicity lest.

~ DUIAdt'aMSDYllfuo: 0.1014 Lov.--.r PMSD bouDd dcunniaed by USE!'A (I Doh pera:olile)

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PMSD: 12.3 U - PMSD bculd cleoanuoed by USEPA (900h percalllle) *30%. Lowu mel upper PMSO bound. won: ddennmed from the IOOh mel 900h potea>lile. mpeajvdy. of PMSD daa from EPA's WET ~ Varilloilily Study ( USEPA, 1001a; USE.PA, lOOI b). USEPA. 2001a. 2001b Fioul Rcpon* lnurlabonuory VariabllityS.udyof EPA Sbon*lerm Oa-onicand Aaae Wbole EftlOCDtToxicity Test Melbods. Volumes I mci2-Appc:adox. EPA-821-8-01~ and EPA-821-8-0I.oo5. US Enn fOfl-l'roooctJoa , . _, CiDCUIDali. OH.. Page 57 of 1 00 Fie: sqn101 050311cfa1a-uv.xlsx

                                                                                                                                                                                                                                                                             ~l>)r. J. Sumner Reviewed l>)r. c:J\-

TVA I Sequoyah Nuclear Plant, Outfall101 UV-treated ! TS

\ ) Environmental Testing Solutions, Inc.

May 03-10, 2011 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/3/2011 Test ID: PpFRCR Sample ID: TVA I Sequoyah Nuclear Plant, OutfalllOl End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cone-% 2 3 4 D-Control 0.7140 0.9080 0.8530 0.8490 10.8 0.9010 0.8130. 0.8670 0.8850 21.6 0.8960 0.7930 0.7790 0.8290 43.2 0.8790 0.8520 0.8470 0.7730 86.4 0.8500 0.7700 0.7740 0.7970 100 0.8160 0.8020 0.6600 0.8400 Intake 0.9480 0.8830 0.9250 0.7990 Transfonn: Untransfonned 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.8310 1.0000 0.8310 0.7140 0.9080 9.930 4 0.8488 1.0000 10.8 0.8665 1.0427 0.8665 0.8130 0.9010 4.417 4 -0.848 2.410 0.1009 0.8488 1.0000 21.6 0.8243 0.9919 0.8243 0.7790 0.8960 6.341 4 0.161 2.410 0.1009 0.8310 0.9791 43.2 0.8378 1.0081 0.8378 0.7730 0.8790 5.419 4 -0.161 2.410 0.1009 0.8310 0.9791 86.4 0.7978 0.9600 0.7978 0.7700 0.8500 4.614 4 0.795 2.410 0.1009 0.7978 0.9399 100 0.7795 0.9380 0.7795 0.6600 0.8400 10.417 4 1.231 2.410 0.1009 0.7795 0.9184 Intake 0.8888 1.0695 0.8888 0.7990 0.9480 7.382 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93555665 0.884 -0.827227683 0.41674318 Bartlett's Test indicates equal variances (p = 0.63) 3.45981002 15.0863171 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 0.10085134 0.12136142 0.00376394 0.00350235 0.406950474 5, 18 Treatments vs D-Control Linear lnterpoJation (200 Resamples) Point  % SD 95%CL(Exp) Skew IC05 75.275 ICIO >100 IC15 >100 IC20 >100 IIC2S >1001 File: sqnl01_0503lldata-uv.xlsx IC40 Entered by: J. Sumner IC50 Page 58100

                        >100
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TVA I Sequoyah Nuclear Plant, Outfall 101 -Intake UV-treated May 03-10,2011 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/3/20 II Test ID: PpFRCR Sample ID: TVA I Sequoyah Nuclear Plant, Outfall IOJ End Date: 5/10/2011 LabiD: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Cone-% 1 2 3 4 D-Control 0.7140 0.9080 0.8530 0.8490 10.8 0.9010 0.8130 0.8670 0.8850 21.6 0.8960 0.7930 0.7790 0.8290 43.2 0.8790 0.8520 0.8470 0.7730 86.4 0.8500 0.7700 0.7740 0.7970 100 0.8160 0.8020 0.6600 0.8400 Intake 0.9480 0.8830 0.9250 0.7990 Transform: Untransformed 1-Tailed Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.8310 1.0000 0.8310 0.7140 0.9080 9.930 4 10.8 0.8665 1.0427 0.8665 0.8130 0.9010 4.417 4 21.6 0.8243 0.9919 0.8243 0.7790 0.8960 6.341 4 43.2 0.8378 1.0081 0.8378 0.7730 0.8790 5.419 4 86.4 0.7978 0.9600 0.7978 0.7700 0.8500 4.614 4 100 0.7795 0.9380 0.7795 0.6600 0.8400 10.417 4 Intake 0.8888 1.0695 0.8888 0.7990 0.9480 7.382 4 -1.096 1.943 0.1024 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.0 I) 0.88838106 0.749 -0.9249599 -0.3380108 F-Test indicates equal variances (p = 0.72) 1.58184743 47.4683456 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.10242295 0.12325264 0.00667012 0.00555646 0.31525472 1, 6 Treatments vs D-Control File: sqnl01_0503lldata-uv.xlsx

  • Entered by: J. Sumner Page 59 of 100 Reviewed by: --tr-
                                            ;.  ;, ,; ; ;j, i-l TVA I Sequoyah Nuclear Plant, OutfalllOl - UV-treated May 03-10,2011 Pimephales promelas Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Environmental Testing Solutions, Inc.

Concentration Control 10.8% 21.6% 43.2% 86.4% 100% File: sqn101_050311chem-UV.xls Page 60 of 100 Entered by: Reviewed by: S:Jfns

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Species: Pimephales promelas Date: OS*<e>- \\ Client: TVA I Seguoyah Nuclear Plant, OutfalllOl, UV-treated lI I CONTROL UV-treatcd .I .I 10.8% I 21.6% I I 43.2% I 86.4% I I 100% I I I 100% Intake .I I Page 61 of 100 SOP AT20- Exhibit AT20.3, revision 04-01 -09

Page 6 of 6

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'I I CONTROL I-.1!:!!.!.!!£:~L_--1--=____:- I 1 10.8% I 21.6% I I 43.2% I 86.4% I I 100% .I

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lntake -I Page 62 of 100 ~ (4to)llN<.' \'ll'fl~ COI-)f\~ I c)~ SOP AT20 - Exhibit AT20.3, revision 04-01-09

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Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL = O.LO mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst~)~ Iodide r~agent ---l:...!rJ:!..i.:=.~..LJ.4-=-l_ _, 1--J Date analyzed 0S:3 II Acid reagent:!L...--..!.\.!.:t-!:.J.~.:..i.J..:2::..;6:..---' li Laboratory controI standard: I Reference standard number True value (TV) (mg/L) Measured value (MV) (mg/L)

                                                                                                              % RS = MV I TV x 100 (acceptable range= 90 to 110%)

lMssqa 1 0.50 0.'-(S) tlo.a.. *t. I Sample characteristics I Sample ID Sample characteristics I I I Note: All samples were analyzed in excess of EPA recommended holding 5 minutes) unless otherwise noted. L a boratorv co11troI stand ard : Reference standard True value (TV) Measured value (MV)  % RS- MV /TV x 100 number (mg/L) (mg/L) (acceptable range= 90 to llO%)

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Reviewed by ~~£~,......,=-~--; Date reviewed 0 S~(?.) *I\ Page 63 of 100 SOP C8 - Exhibit C8. I, revision 09-0 t-09

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Total Residual Chlorine I (Orion Electrode Method, Orion 97-70) Matrix: Water, JU, =*o.IO mg/L Meter: Accumet Model AR25 pH/Ion Meter

                                  ,,_~*J=-----i                                                              Iodide reagent: r\tJ:.:...~~4:-.:.4-:-=:1-~---1 j:

Analyst Date analyzed 06 .06 .11 Acid reagent: . ( C\112-Y iJb La boratory controI standard: Refe rence standard True va lue (TV) Measured value (MV)  % RS = MV I TV x 100 number (mg/L) (mg!L) (acceptable range= 90 to 110%)

          \l\JSSC1 1.\                                   0.50                 0.5~ 1                               10\Q .t.Jl~

Sample characteristics Sample characteristics 2.06(, Note: All samples were analyzed in excess of EPA recommended holding time (IS minutes) unless osre~_:~ noted . .1 ~ L aboratory co11troI stall dard : -i. 7-S ..-Q.~ ..J:r lOO.-It~ Reference standar d True value (TV) Measur ed value (MV)  % RS =MY I TV x 100 number (mg!L) (mg!L) (acceptable range= 90 to 110%)

         \NSSqvt                                        0.50                    o.~9H>                              q~ .O'liJ Reviewed by Date reviewed I a=~   OS*\\

Page 64 of 100 SOP C& -Exhibit C&.l , revision 09-01-09

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Environmental Testing Solutions, Inc. Total Residual Chlorine (Orion Electrode Method, Orion 97-70) rlYllif.-; Matrix: Water, RL = 0.10 mg/L I Analyst Meter: Accumet Model AR25 pH/Ion Meter [odide reagent: 1~-='1-:::...N:.....::..;;;~_,__,.~-'------; Date analyzed ()?;;;; . \l Acid reagent: . "!-t-Jt.it'Zt~ L ahoratory controI standard: Reference standard True va lue (TV) Measured value (MV)  % RS = MV I TV x 100 number {mg/L) {mg/L) (acceptable range= 90 to 110%)

                          -r:..~*~)qz., \                                         0.50                   0.'54&\.                         t[)Gt.&1.,

Sample characteristics Sample lD Sample characteristics 11 Note: All samples were analyzed in excess of EPA recommended holding time (IS minutes) unless otherwise noted. L ahoratory control stan dard: Reference standard True val ue (TV) Measured value (MV)  % RS = MV I TV x 100 number (mg/L) {mg/L) (acceptable range= 90 to 110%)

                         ""LN.) S'\'UI                                           0.50                 o. '50()                          IOO.o*l, Reviewed by '1-""""""~;:Jt~~.......,,..,.---1

- 1. Date reviewed ........,....;;. OS;::;.~-'* L..:..*....;l.....l_ __, Page 65 of 100 SOP C8 - Exhibit C8.1, revision 09-01-09 I,

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Alkalinity (SM 2320 B) Matrix: Water, RL = LO mg CaC03/L Analyst ~.J..&l=S~l-:::,___ _--ll Time initiated I j D5o Date analyzed D5-05-1l l Titrate samples to Time completed Ll-~~ta:o~:l~===~~~ pH=4.5 S.U. determination: Normality (N) of H2S04 pH Factor or Multiplier Begin End Total = (5 ml NazC03 x 0.05)/E = (N x 50000)/ 100 ml sample ml ml ml =0.25/E =Nx 500

                                                                                                                     = 0.0180-
                                                                           \~ .(.?   l~.IQ Alkalinity (MV)      % RS= MV /TV x 100 End        Total       Multiplier       (mg CaCOJIL)            (acceptable range ml                                                  =90 to LIO %)

Multiplier Duplicate (B)

                      \                                                      D.D                 3.1 Matrix spt'ke recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO)!L)

                                          ~CaCOJIL)                (ml)        ml       ml          ml
rNSS ~~3 (aJ~ too o.o -:J-.IP -=1-.\R C! .q '=t5 Sample alkalinity (B) Measured spike value (MV)  % R = MV I SV x 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaCO)I'L) = 75 tol25%)

31 ~ '6~tfJo samplel measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (me CaC031L) DSL"h.U ml+~w \DO -:t.~..o 13.$ 5.9 Cf.q 5'~

    ,..,. .... :{' M              as <n*tiA                                                    16.5 IC!.5 l.?.O                                        sq t:::i>tC ' "        . ~0' iiodl-d.B                                                        \<l5" ;),5.<:; lo.o                                    5a tJq._z.l, I)                                                                              ~5.5 31.5 Co.o                                           S'j 0~-U.                I\                                                                   3\.5'        31R        (Q.3                             ltZ...

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Alkalinity (SM 2320 B) Analyst Date analyzed

                                    ~_.fa=:.__------l l>5* 08. 11 Matrix: Water, RL = 1.0 mg CaC03 /L Titrate samples to Time initiated Time completed _

I~

                                                                                                                                                                         '""""~L i

pH == 4.5 S. U. Titrant normality and multiplier determination: J!.R of Normality Normality (N) of H2S04 pH Factor or Multiplier Deionized Titrant c ...,...... D End Total (5 ml Na2C03 x 0.05)/E = (N x 50000)/ 100 ml sample water reference standard ml ml 1111 0.25/E =NxSOO

    =4.5S.U.              number                 number                                _(_E)        (acceptable range-_v.           11 nno)
                                                                                                                                                                      ~~

Laboratory co11trol sta11dard: . .. Reference standard True value Sample Alkalinity (MV)  % RS =MV I TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC{h/L) (acceptable range (mg CaCOy'L) (ml) ml ml ml =90 to 110%) J:'IJ'i;~ '()~ 100 100 )5.2-- ~.,

> G)_) C\.1 C,o qo.o0 lo I Alkalinity %RPD=

Multiplier (mg CaCOJIL) {(S- 0) / [(S+D)/21} x 100 I s q (acceptable range=:!: 10%) D I a r1X sp1 e recovery: Reference standard Spike value Sample Spike alkalinity (A) I number (SV) (mg CaCOJIL) volume (ml) Begin ml End ml Total ml Multiplier (mg CaCOJIL)

 .1"NS5 '5~~                                  5'0                   1'0 0    3?>.1-   1m             ~IIi .

1k/_ q_q ~ IJ-t O I Sample alkalinity (B) (mgCnCOYL) Measured spike value (MV) MV =A-B

                                                                                                                                       % R = MV I SV " 100 (acceptable range

_(_mgCaCOy'L) =75 to 125%) q~ r+-=t C\~.D~I . . samp1el measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mgCaCOYLl

  ~.\\                            ~~ ~suJ                                      LOO                 o.o          q , ~,     Cl.IP         q_q                 qs
  <1)S.OZ-liA                              I                                                       q,\p       '2.1.1.1     ~~.0                             120 IIOSOo.~ '                     lDaJl Cu.L l                                                     21.1P       :33 .~      (1.4-                             liD II 0'Sb ~
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           ..                                                                                                                                                           Page~

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j j Alkalinity (SM 2320 B) l

 ~

Analyst I Bst-Date analyzed 1--'0::::.S':::..::*:::...O-~-

                                                         . ,-,-----i Matrix: Water, RL = 1.0 mg CaC03/L Titrate samples to Time initiated§::

Time completed 1--===--~---1 c:=

  • I
 ;                                                                                          pH=4.5S.U.
 !          ttrant normality and multiplier determination:

nil .l' Nnrn111 1itv Normality (N) of H2S04 pH Factor or Multiplier Deionized Titrant check Begin t:na l u ... . ' '" ml Na 2 C03 x 0.05)/E = (N x 50000)1100 ml sample I water

         = 4.5     s.u.

reference number standard number ml ml ml (E)

                                                                                                                   -;;;,t..l.::ll"'

(acceptable range - 0.0180- 0.0220) --

                                                                                                                                                                 -~x 500 Laboratory controI stan dard:

Reference standard True value Sample Alkalinity (MV)  % RS = MY I TV x 100 number (TV) volume Begin End Total Multiplier (mgCaCOYL) (acceptable range (mg CaCOYLJ (ml) ml ml ml =90 to 110%) j:NS;~ 100 100 1\ .q q .'2. '1 .4 GJI .D.../.n.~nL *~

                                                                              ~ ."1-                                                        C\1                       '    ~BSI .      !C".c_

Sample Alkalinity %RPD= Sample ID volume End Multiplier (mg CaCOYL) {(S - D) /[(S+D)/21} X 100 (acceptable range= :1: 10%) - }. z.

   ~ '  M4atnx spike recovery:
   ~

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOYL) (mgCaCOYLJ (ml) ml ml ml

    '   :rrv&.S~g                                      \00           5o       l-4-.4    ~~s           :J.lR iz\t1.CJ                               )50
   ~                                                   Sample alkalinity (B)

(mg CaCOVL) Measured spike value (MV) MV=A - B

                                                                                                                                          % R = MV I SV x 100 (acceptable range
   ~

(m!!CaCOJIL) =75 to 125%) 5+- C\3 CJ3.o"Jo samp,le measurements: I] Sample number Sample ID Sample volume

                                                                                 . (ml)

Begin ml End ml Total ml Multiplier Alkalinity (meCaCOVLJ tl u~o<... \5 h\1 A- -SQ0 \ ~\ 3 t~ ~.5 OlS:v -:3.1 (~\ tj'_t:j lQl (J f\ ~~ C:,.tn.

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Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Analyst ~.....f'§~L.:___ _- ; Time initiated ~-::::::::=-Jt..t..-----l Date analyzed bs*. cg . 11 Titrate samples to Time completed L - - - - - - - ' pH=4.5 S.U. Titrallt normality and multiplier determination: bli.of Normality Normality (N) ofH2SO~ pH Factor or Multiplier Deionized Titrant checK DO:!;IJJ "'* _Total

                                                                          -=-                     = (5 ml Na1C03 x 0.05)/E                 = (N x 50000)/100 ml sample water            reference                standard       ml         ml       ml                     =0.25/E                                    =Nx 500 4.5S.U.           number                   number                           (E)      (acceptable range 'u.u l<.v-
                                                                                                                                                     -~

L ab oratory controLstan dard: Reference standard True value Sample Alkalinity (MV)  % RS= MV / TV x lOO number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJIL) (ml) ml ml ml =90 to 110%) J:"N"S.S <b~3 100 100 11.5 '*> .~ ~u 4 .<1 ceo qo .ovfo Alkalinity %RPD = Multiplier (mg CaCOJIL) {(S- D) /[(S+D)IllJ x 100 (acceptable range=:!: 10%) Mit.a ru: STJl'ke recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (mg CaCOJIL) (ml) ml ml ml

  ;::r~ss~3                                       ICO         so          ~:3 .v              1-.~ 1, 1!-) Cl.'1      .l; ~                 ISO I

314 Sample alkalinity (B) Measured spike value (MV)  % R = MV I SV x 100 (mg CaCOJIL) MV=A-B (acceptable range ~( (mg CaCOJIL) = 75 to L2S%)

                                                            ~9                                  cu                                     Ct\ .0°Io samplel measurements:

~* Sample volume Begin End Total Alkalinity Sample number Sample ID {ml) ml ml ml Multiplier (riig CaCO,VL)

   \ \ DScn..       en..        rrJA ~..J bJi lA.) \                          ~             314        34:o       .;?,(.:. 2.) q .C(                   ~11..

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Page of_4 I t! Analyst I~ I Total Hardness (SM 2340 C) RL = 1.0 mg CaC031L Time initiated I 0 ~ Ol.f* I Date analyzed o5 _ 0-g *11 Time completed ~~l:o=l-f:.o~======: ~ 1 LC...~ O\lT G~ AtC:.etmr-lte.. LIMIT'S. Titrant normality and multiplier determination: ASC:.o<.J f\Te. t) S.AH~~ .tt AI'J AL'I 'H~ t>- ~ e j)A~ ~ Titrant Normality check Begin End Total Normality (N) of EDT A pH Factor or Multiplier 1 reference standard ml ml ml =0.2/E = (N x 50000)/ 50 ml sample number number (E) (acceptable range= 0.0180- 0.0220) =Nx 1000

       *.rtJ jt .y..23 ..:r.vss ~crcg                        D.l          cr.q        q;g                     0.020~                                              2D.lf L ahoratory controI stan dard:

Reference standard True value Sample Hardness (MV)  % RS= MV /TV x 100 number (TV) volume Begin End Total Multiplier (mgCaCOYL) (acceptable range (mg CaCOJIL) (ml) ml ml ml =90 to 110%) 40 50 c, ,q II .lop q

                                                                                                       '"'1Qs,v TIVSS. tp1G)                                                                                                 ;.t..()~             ..JI                        "'lC.."V-10

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Sample Hardness %RPD= ' *r Sample Sample ID volume Begin End Total Multiplier (mgCaCOYL) {(S- D) /[(S+D)/21} x 100 ml I os.os.tJ

                     ~

Ssw Duplicate (B) I:'"} 2u.c.J. D 35 1.-:f J.. :35 Miatnx . sp1'ke recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Mtdtiplier (mg CaCOYL) (mgCaCOYL) (ml) ml ml ml ~ r tJ$5 cw1 140 5o 13..3 (1.0 3.-:t- 2i).Y. ~.\t.(o) Sample h.ardness (B) Measured spike value (MV)  % R=MV /SV x 100 (mgCaCOYL) MV=A-B (acceptable range (mgCaCOYL) =75 to 125%) 35 ~'1\ 168. o~t'o * ( \oz..~*?) s,ample l measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (me CaCOJIL) TV=ND Blank (should be= 0 mg CaCOJIL) ff) t-:} ,i) 11-.o O.o z~ .Y. f0() bS- oS-1> (n\1$\)J I \-:f.i) o:>J.l. J-t .'2. ~~Z4o o~~01*1 \A ,;1 \.~ .;(5.; Jt.l <64-OS .lf.\ . \\ ~ ~.3 ~Cf.'5 LJ-.2... '8'(o l)lt'2.1*') ~tf.S $5.1- J-t. 2., ~ b'-{ .l.-(pJ\ 33.1- 3~ .0 ....... 3 <g~ 06.02--l \ A 3$'0 ~.2 4-.?... ~~ tt'S.O'Z-1 \ ..(3 4~.?. 1~*3 . \ 'iN* os.o.3.t\

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     ~cfgl7Nolo~t8r(ft is used, sample must be diluted.                         Reviewed by:                                              Date reviewed       I    os-o& -II SOP C7- Exhibit C7.1, revision 09-01-09

35 iETS Page Page 4 of~-

      )   Environmental Testing SoJutlons, Inc.

Total Hardness (SM 2340 C) RL = l.O mg CaCO/L Analyst ~i!..<Rpl..=-----1 Time initiated ~............._ Date analyzed 0 5 . c 8 . 11 Time completed t---=-~=- -:-----1 1 rant normartty an d mu lt'l' T.'t :tpper determ nation: Titrant Normality coeCK F.nd Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml mJ -0.2/E - (N x 50000)/ SO ml sample number number (E) (acceptable range *u. n A')'l0) =Nx 1000 Laboratory controI stan dard: Reference standard True value Sample Hardness (MV)  % RS= MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaCO,!L) (ml) ml ml ml =90 to 110%)

!N$ q 1 'I 40 50 5.~ 1-.1- J.q 2-o.~ 3'1 'i:J ,Sb/o Hard.ness %RPD=

Multiplier (mg CaCO,!L) {(S

  • D) /I(S+D)/2)} x 100 s

D Mi.atrlx sp_t'ke recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (mg CaCOJIL) (ml) ml ml ml

I"NSS<11q 4~ Sv \d-.4 lq,D ~.lo 2,1),\f- ~IYO Sample hardness (B) Measured spike value (MV)  % R = MV I SV x 100 (mg CaCO,!L) MV=A- B (acceptable range
                                                                                                                                   =75 to 125%)

s amp~e l measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (me CaCOJIL} TV~ Blank (should be = 0 mg CaC03/L) llCSOS. 0'7- L.l.h..Uw 1. 8) 1<1.0 cQ3.1 ,Lf .'} Zo~ C}tp 1\ ()$()1. 0'1- l 3 I ~3 . 1- ~<?.5 ~.~ 9~ lnili'Qf'\Oo~o.. l i n o$~ . 0"2-l\ o 'S>CJS

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Envlronmtntaltening SoJutfons,lnc. Page 3 Page 3"' of_'+ __ Total Hardness (SM 2340 C) RL = l.O mg CaCOiL Analyst Date analyzed I f36L-t-()-"'$=.t>'-8---, -,----1 Time initiated 'b..,_ Time completed ll--=-c::::::::::::::o::::::::::--

                                                                                                                                                                                  -:---i Tt1trant normality and multiplier determination:

Titr,.nt Nnrm<>litv check Begin End Total Normality (N) of EDT A pH Factor or Multiplier

                                                                                                                                                  = (N x 50000)/50 ml sample reference number standard number ml           1111
                                                                                      ~ (Ef
                                                                                                                       =0.2/E
                                                                                                              ...le range 0.0180 - 0.0220)                      Nx 1000 I

L aboratory controI standard: Reference standard True value Sample Hardness (MV)  % RS = MV I TV x lOO (acceptable range number (TV) volume Begin End Total Multiplier (mgCaCOYL) (mg CaCOYL) =90 to 110%) (ml) ml ml ml

       .:I'NSS<=hc\                               40               50           \....2.    +_q         i.-=i-         20:4             35                       ~+-~1.
  '                                                     Y~ *.                  -~.C\       G.~        I. q             w.~*            3'1                       &f1.~ 0/o
 'i Hardness                  %RPD=

Begin End Total Multiplier (mgCaCO)!L) ((S - D) /[(S+D)/2)} X 100 ml ml ml 2c.~ s ~~ 12~ 3.\ D j_ 1.3 lle*0 3.\ I atrrx sp1 e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaC03/L) I J'rJS6CjiCJ (mgCaCO)!L) l-l-0 (ml) 5o ml 11..CJ ~!l ml S:o ml 2\:14 11::>0

                                                                                                                                       % R = MV I SV x 100 l'

Sample hardness (B) Measured spike value (MV) (mg CaCOJIL) MV=A-8 (acceptable range (mgCaCOJIL~ =75 to 125%) II I Sa1tlp_Je I measurements: v3

  • 3'1- q2.5°/o Sample volume Begin End Total Hardness I TV Sample number ND Blank" Sample ID (should be ' u n (ml) ml ml ml Multiplier (meCaCOYL)

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I ..._I Date reviewed I SOP C7- Exhibit C7.1, revision 09-01-09

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          !ETS Page Page       4        of_4~*__
}          ~   Environmental Testing Soh.rUon.s. fnc.
~l i
 '                                                                             Total Hardness (SM 2340 C)

I! i Analyst Date analyzed Ir-()= f£4.. 5

                                                   -.c:g
                                                      --.-1\---;

RL = 1.0 mg CaCOi L Time initiated I Time completed 1-........_.;;::::o..,.<::::::::::i~-

                                                                                                                                                                         ---i l           itrant normality and multiplier determination:

u *. ""* **"'check Be~in End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml m1 0.2/E (N x 50000)/50 ml sample (acceptable range "* Nx 1000 number 1111?')0) number (E) L ab oratory controI stan{/,ard: Reference standard True value Sample Hardness (MV)  % RS=MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaCOJIL) (ml) ml ml ml = 90 to 110%)

        .'IIVS:Sqi1                                    40            50       IR6 Ra.t          l.q         2-D !-/         3q                        cn-sr;,lo Hardness                    %RPD=

End Total Multiplier (mg CaCOJIL) . {(S- D) /((S+D)/21} ll 100 Total Multiplier Sample hard.ness (B) Measured spike value (MV)  % R = MV I SV x 100 (mg CaCDJ/L) MV=A*B (acceptable range _(mg CaC01/L) =75 to 125%) cro J.i'tl \o0,1) ~/o samp1e l measurements: Sample volume Begin End Total Hardness Sample number SampleiD (ml) ml ml Multiplier

   'i ml                               Cm2 CaCDJ/L)

TV -:-li.Tr\ Blank SDOUIU

                                                              ~. n                                                                             i 1\ Ob H. . 6'-\              Drn a..           dd AC.                b             /f5 .i   ~~-lc>     '+.'5        k_\). tJ               qz.

I \ {>pf\C I .;1~.0 Jl";f.C '+~ i qo

                  .L                                  I   {)Pet-                 I            d'i-.0    31.~p                  J-I                                                                                                              J.f.""                              CJ4-I                                                                     v---
                                                                                  ~

-I ~

                                                             '-                                                                     -'{350 I  ~a<>gi:tf35~¥>~tty(jlt is used, sample must be diluted. Reviewed by: _Ba.=b'--::..__ _ _....J
                                                                                             ._I                             Date reviewed Q5.Qq.L \

I SOP C7- Exhibit C7 .l, revision 09-01-09

                *Ers I

ll* f **

                 ~   >              '

J f'* *. i~'" 1

 .1             ~ etlvfronmtnt111Ttitlng Solutkms, fnc:.
.'3 Total Hardness (SM 2340 C)

Analyst 11/i£ RL = 1.0 mg CaC03/L Time initiated Ioqa5

  • Date analyzed O~Oi.l 1 Time completed  :=o:~~':jf)=======:

Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier J reference standard ml ml ml =0.2/E = (N x 50000)/ 50 ml sample l number number (E) (acceptable tange = 0.0180- 0.0220) =Nx 1000 e. c1'-1 ~2.'3 IN5Se1~ 0.0 C\~ qq Q.ozo2. ~o.,.. Lb a oratory controI standard: Reference standard True value Sample Hardness (MV)  % RS- MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJIL) (ml) ml ml ml =90 to 110%) IN') SCilti 40 50 ot.~ 1\.9 A-0 ~.~ ~ &.{0 IOO.o'/. Sample Hardness %RPD= volume Multiplier (mg CaCOJIL) {(S -D) /[(S+D)/2]} X 100 20.2, s D MiatrTX . sp1'ke recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mgCaCOJIL) (mgCaCOJIL) (ml) ml ml ml l ~SSl1 lti 40 R) J~.o J~o Lt*O -zo.z. et Sample hardness (B) Measured spike value (MV)  % R = MV I SV x 100 (mgCaCOJIL) MV=A*B (acceptable range (mg CaCOJIL) ~ 75 to 125%)

                                                                       "iZ..                            :3q                                £11-.S/:

s.amp,le measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (meCaCOJIL) Blank TV=ND (should be= 0 mg CaCOJIL) 9.) o.o O.o o.u ~0.2- r"'-J I 05.QS.\l OS. (1:\-~ll A ll'S .\f:\. II 0 IYI~1'-lli 18.0 a-s ln.l 2.'1..'5

                                                                                                          ?.':\ .t &.\.v
                                                                                                                        &.ts "l.')

C)\ q~ ql 04 *2.1.\\ 3l.\l ' *"'

                                                                                                           ~.o       '-t.q                           t¥l 04*fu .\\                                                                          ~.0       qful       4.4                            ~~.

0'5 .ctl.*l fi A~.CI -ao. l ~.~ C11 o~/.o1--t 18 ;o.\ 3'1.5 q,cj BCi 05.0~.,, v 3-t.S 3q,z_ ~*1- Gt$

              ~.OZ..\\                     Ssu                                        ~lr         ~*s u/.~ Z..t                     ,I;              i.IZ..

p~~~ 551f <>feJG11t is used, sample must be diluted. Reviewed by: L----r'::lt-'\_ _-1 D'\ Date reviewed I 'OS.* c'\* 11 SOP C7- Exhibit C7.1, revision 09-01-09

Sequoyah Nuclear Plant Biomonitoring May 03- 10,2011 AppendixD Reference Toxicant Test and Control Chart Page 75 of 100

     '~

Pimephales promelas Chronic Reference Toxicant Control Chart

        )

Organism Source: Aquatox, Inc.

     ~    Environmental Testing Solutions, Inc.

USEPA Control Limits(+/- 2 Standard Deviations) 0.9 0.8 ..... ............................................ 0.7 0.6 0.5 1.4 -u

~

1.2 1.0 USEPA Warning and Control Lim.i ts (75th and 90th Percentile CVs)

~

'bo u 11'1 N 0.8

>         0.6 "C

I 0.4 1.4 Laboratory Warning and Control Limits (lOth and 25th Percentile CVs) 1.2 1.0 0.8 0.6 .................................................................................................. 0.4 Test date

  • 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

Central Tendency (mean IC25 } Warning Limits (mean IC25 +/- sA.lO or sA.75) G~a phs aenel'ited from associated excel spreadsheet. Control Limits (mean IC25 +/- SA.25, SA.90, or 2 Standard Deviations} Excel spreadsheet entered by: J. Sumn*r Reviewed by: --¥---

I, @11 G ~ ETS Environmental Testing Solutions, Inc. Plmephales promelas Chronic Reference Toxicant Control Chart State and U5EPA laboratory laboratory U5EPA U5EPA Test number Test date 7-daylC,. cr 5 Control Limits Warning Limits Control Limits 5.._75 Warning Umlts 5.._.. Control Umlts cv (g/LKCI) (g/L KCI) CT-25 CT+25 cr- 5.uo cr + 5.uo cr-5..., cr+5..., cr-5.._75 CT+5A.75 cr-5.._.. CT+5.uo 1 09-14-10 0.75 2 09-21-10 0.73 0.74 0.02 0.71 0.71 0.09 0.65 0.83 0.16 0.58 0.89 0.28 0.46 L02 0.33 0.41 L07 0.02 3 09-28-10 0.65 0.71 0.05 0.60 0.82 o.os 0.62 0.79 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.08 4 09-29-10 0.68 0.70 0.05 0.61 0.79 0.08 0.62 0.78 0.15 0.55 0.85 0.27 0.43 0.91 0.32 0.39 1.02 0.07 5 lQ-05-10 0.70 0.70 0.04 0.62 0.78 0.08 0.62 0.79 0.15 0.55 0.85 0.27 0.43 0.97 0.32 0.39 1.02 0.06 6 1o-o6-10 0.72 0.70 0.04 0.63 0.78 0.08 0.62 0.79 0.15 0.56 0.85 0.27 0.44 0.97 0.32 0.39 1.02 0.05 7 1Q-12-10 0.70 0.70 0.03 0.64 o.n 0.08 0.62 0.79 0.15 0.56 0.85 0.27 0.44 0.97 0.32 0.39 1.02 0.05 8 lQ-26-10 0.68 0.70 0.03 0.64 0.76 0.08 0.62 0.78 0.15 0.55 0.85 0.27 0.43 0.97 0.32 0.39 1.02 0.05 9 11-()2-10 0.70 0.70 0.03 0.64 0.76 0.08 0.62 0.78 0.15 0.55 0.85 0.27 0.43 0.91 0.32 0.39 1.02 0.04 10 1Hl9-10 0.75 0.71 0.03 0.64 o.n 0.08 0.62 0.79 0.15 0.56 0.85 0.27 0.44 0.97 0.32 0.39 1.02 0.05 11 12-<17-10 o.n 0.71 0.04 0.64 0.78 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.05 u 01-18-11 0.78 0.72 0.04 0.64 0.80 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.06 13 01-25-11 0.70 0.72 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.05 14 02-QS-11 0.69 0.71 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.04 0.05 15 02-15-11 0.68 0.71 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.05 16 03-os-11 0.71 0.71 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.05 17 03-os-11 0.70 0.71 0.04 0.64 0.78 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.05 18 03-29-11 0.75 0.71 0.04 0.64 0.78 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 o.os 19 03-31-11 0.75 0.71 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.05 20 05-03-11 0.78 0.72 0.04 0.64 0.80 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.05 Note: 7-d IC,. ~ 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. CT ~ Central tendency (mean IC25). 5 ~ Standard deviation of the IC25 values. laboratory Control and Warning UmJts Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the lOth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. S.uo ~ Standard deviation corresponding to the 10"' percentile CV. (s._., ~ 0.12) 5...,. ~ Standard deviation corresponding to the 25"' percentile CV. (S..,. ~ 0.211 U5EPA Control and Warning Limits SA.75 ~ Standard deviation corresponding to the 75"' percentile CV. ( S._15 ~ 0.38) sASO~ Standard deviation corresponding to the 90"' percentile CV. (S....., = 0.45) CV ~ Coefficient of variation of the IC,. values. USEPA. 2000. Understanding and Accounting for Method Variability tn Whole Effluent Toxldty Applkatlons Under the National Pollutant Discharge Elimination Prosram. EPA-833-R~3. US Environmental Protecllon Aaencv~ Ondnnatl. OH. rile: ppkda_o5o3u.x~sx Page 77 of 100 l!llleredby:J.s'T Organu~m obtainedfrom Aquotox.Inc. R<vicwcdby: ~

Pimephales promelas 1'

          *~

Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements

         *~     Environmental Testing Solutions, Inc.

Organism Source: Aquatox, Inc. 1.00 0.75 0.50 0.25 USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)

    ~

r : .r: 30 Kentucky Acceptance limit(< 30.0%) 0 i0

     "' (,!)...
    'i::           20
    >"' 0 0 ...

r: Gl

    'u r:

8... 10 EGl .E *

  • Ill * * * *************

0 0 u 30 USEPA Upper PMSD Bound (90th percentile < 30.0%)

  ~
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   ~

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                                             *** * ***** *** * *** ** * ******************** *** ** ****** **** t ********* ** ******** * ******** * **** ** * **
  • Control Reproduction, Coefficient of Variation (CV}, or Percent Minimum Significant Difference (PMSD} PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

Central Tendency (mean Control Growth, CV, or PMSD)

      ......... Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)

Page 78 o 100 Graphs 1enerated from as.soclated exc~t spreadsheet. Exeel spreadsheet enKred by: J. Sumner Revlowe<f by: Q)

iETS Precision of Endpoint Measurements Pimephales promelas ) Envlmnmental Testing Solutions, Inc. Chronic Reference Toxicant Data Control Control Mean Test number Test date Survival Growth cr cv cr MSD PMSD cr for Control Growth for Control (%) (mg/larvae) (%) (%) forPMSD(%) (mg/larvae) Growth CV (%) 1 09-14-10 97.5 0.813 5.9 0.11 13.9 2 09-21-10 100 0.891 0.852 7.5 6.7 0.07 7.5 10.7 3 09-28-10 97.5 0.991 0.898 9.5 7.7 0.11 10.9 10.8 4 09-29-10 100 0.893 0.897 8.5 7.9 0.11 12.1 11.1 5 10-05-10 100 0.912 0.900 8.1 7.9 0.09 9.5 10.8 6 10.06-10 100 0.810 0.885 2.3 7.0 0.06 7.4 10.2 7 10-12-10 100 0.799 0.873 4.1 6.6 0.10 12.0 10.5 8 10-26-10 100 0.779 0.861 10.0 7.0 0.13 16.8 11.3 9 11-02-10 100 0.727 0.846 10.9 7.4 0.11 14.5 11.6 10 11-09-10 100 0.761 0.837 6.3 7.3 0.12 15.4 12.0 11 12-07-10 100 1.080 0.860 8.3 7.4 0.15 *13.9 12.2 12 01-18-11 97.5 0.806 0.855 5.8 7.3 0.09 11.0 12.1 13 01-25-11 100 0.608 0.836 4.6 7.1 0.08 13.2 12.2 14 02-08-11 100 0.817 0.835 2.6 6.7 0.06 7.8 11.8 15 02*15-11 100 0.701 0.826 3.3 6.5 0.12 16.8 12.2 16 03-08-11 100 0.953 0.834 3.1 6.3 0.08 8.4 11.9 17 03-08-11 100 1.006 0.844 4.7 6.2 0.13 12.7 12.0 18 03-29-11 100 0.829 0.843 6.3 6.2 0.09 10.3 11.9 19 03-31-11 100 0.675 0.834 3.2 6.0 0.09 12.8 11.9 20 05-03-11 100 0.867 0.836 6.3 6.1 0.08 8.8 11.8 Note: 01 ~ Coefficient of variation for control growth. Lower 01 bound determined by USEPA (lOth percentile)~ 3.5%. Upper 01 bound determined by USEPA (90th percentile) ~ 20% MSD ~ Minimum Significant Difference PMSD ~ Percent Minimum Significant Difference PMSD Is a measure of test precision. The PMSD Is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10m percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile) = 30%. CT ~ Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-Q0-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-8-01-QOS. US Environmental Protection Agency, Cincinnati, OH. File: ppkclcr_OS0311.xlsx Entered by: 1. Sumner Reviewed by:---+-

I I ~ETS i Page I of5

     ~ Envlronrru~nt-.JT*nlng Solvtlonr. Inc.

I I I Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas 1 I PpKClCR Test Number: ~ Dilution preparation information: Comments: I KCl Stock INSS number: Stock preparation: INSS. '\'2.Q-SOg KCIIL: Dissolve 50 g KCI in 1-L Milli-Q water. Dilution prep (mg!L) 450 600 750 900 1050 I Stock volume (mL) Diluent volume (mL) Total volume (mL) 9 991 1000 12 988 1000 15 985 1000 18 982 1000 21 979 1000 I Test organism information: Test iliformation: Organism age: '2.1..1~ \\O\l4t.C:. 0\.b Randomizing template: R.e..b - I Date and times organisms OS*CS'l.*\\ \\oQO Incubator number and were* born between: shelf location:

                                                                                                                             .3F Organism source:                                                                Artemia CHM number:        CHM~~
                                                      .-.._"Tl)y. 6Pm..~ ~f OS-<n.*\\

I Transfer vessel pH= '"'l.t. ~ s.u. Drying information for weight determination: Date I Time in oven: 0'*10*\\ ,~10 information: Temperature = 1.~* "1... oc Initial oven temperature: \oO'( I Average transfer volume:

o. ,,,... ~ Date I Time out of oven: cF..*I\~1\ l**hs Final oven temperature: \oO 'C.

Total drying time: 'l.'-t*\~ov<<!:. I Daily feeding and renewal information: Day Date Morning feeding Afternoon feedi.ng MHSW I 0 I 2 I 3 4 5 I 6 7 ~ I I Page 80 of 100 SOP AT21 - Exhibit AT21.1, revision 04-01-09

           ..ETS 3

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            ~
                              '~
                                      ,..___.;-7r' EnvlllmmontollUting 5ollllian$.1n<.

Page 2 of5 Species: Pimephales promelas PpKCICR Test Number: ~ Survival and Growth Data Day Corttrol 450 m~ KCIIL 600m~ KCIIL A B c D E F G H I J K L 0

                                                                      /0       I()     /()          10       /()   10        l()    It)       ID 10           I()      10 1

10 to fO to (0 10 10 lb tO lD tO ro 2

                                                                      /0      10          10         to to         /()     10      10         IO      IO     10 *ro 3
                                                                    /0        10      10           I()     10      I()      I()    10        tO        /Q     10       10 ii-                                   4 IQ      10       10             /Q    IQ       10        10     /Q       IC /0 10                 ID

~ I 5

                                                                    /()     lO        10           (()     to      10       IC     l()       gltl.. ~~ . ~tA..         ~
                                                                                                                                                                         'ct.

6 ,.II(

   '~                                                                ID       10 IC                to       lO              /Q    ID          ~       &'1.     'l    '\
                                                                                                                    ~ '"'

7 ll..fr

                                                                    /D       IC          (()       to      to              /C      lC         g      4        '\      'i '"'

A= Pan weight (mg) Tray color code:: Pl-1&11.1. Analyst: M~ 13*t1 14.fl~ 14-.~ l4.0£t 14-.c;; t3.'H t;.1-l l4.t~ 14.~? t+.4r t;~ot \4-At, Date: fl S! 9:! -'l(ij ~s-<>4-*ll I B = Pan + Larvae weight (mg) Analyst: cDI ~.30 u.1't tS*II1 *v..s* it !It 2t.tM ~~ 22.6'1- 121.2'f n..'fe6

    '!fr' Date:            Q~.~~.u C = Larvae weight (mg) = B- A izt.OS 2l.SO
                                                                                                         ,,,\.

I' Hand calculated. Analyst: Hand -* ejl...._ Weight per initial number of larvae (mg)

          = C /Initial number of larvae g.~~
                                                                    ,_e..,

8.1.'\ t;.'\1 .tAl.

                                                                             ,..,.,~  ~~"'         ""'1,
                                                                                                             ~

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                                                                                                                                     ~~
                                                                                                                                            '-0
                                                                                                                                               "'" o*" ",, o*
                                                                                                                                                        ,..~
                                                                                                                                                                       \:~

I Analyst:

                                 /'"

lJ' Average weight per Initial Percent 0' o* ()' (). ()* a o* \)' 0' number oflarvae (mg) reduction o.&~o, o.1t'2. 'i.& 1. o.1~ l1. t 7. I from control (%) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. I Comments: I

 -   I I       Page 81 of 100 SOP AT21 - Exhibit AT21.1, revision 04-01-09

I i~J, ~ Page 3 of5 Ii )!£; . ~

          } E"nvl.,n....,taJTulfngSollllfono.ln<.

/ ~ l Species: Pimeohales oromelas PpKClCR Test Number: 1.'!. \ I l j Day 750 mE KCIIL Survival and Growth Data 900ml! KCIIL 1050 m KCIIL 'l M N 0 p Q R s T u v w X 1I 0 It> lb /0 IO I() /0 10 lb /0 10 10 IO 1

                                                                  /0      IC                , tf\      I()     /0        ,,tA     10            (o~       arA i *k\          c..~

It> 2 ID IO 10 ~ /0 10 'I 'i'~ lo i ,~ (a

   '                                                                                                   tl\.A ~'~ '\                            s'~ S~~.. ~                      *~

3 'i'G(, ~ JO 4\'"' ~

tf.

4 10 "\ '\ ~ ~ '\ ~

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5 5 ID Dt q '\ ~

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g. t\ 3
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s'f\ ~~

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6 ~u( ~.:,*( bt.l ~ '4't.( ~

                                                                           '\    '\        '\          (..                          i        3 7                         q'" ~ t\       ~

ql$1'1 ~ "" (p (, t""' .gf#t ~ IV\

                                                                                                                                                                   *2P\        ~c(
                                                                                                                                                                ~~           '-
-I       A- Pan weight (mg)

Tray color code:: t'wM Analyst: m~ t+.~s 13.01 1+.4& 14A4 13*.;t.. l4-A*S 13.'ft *~4 l4At t';.fl t5A? 13.0~ IIF Date: Q~*DA*II. B - Pan + Larvae weight (mg) (!,OJ"' u, .lt+ 111-:1-tP Analyst: 200-l 20,\gf' 2.2.-IS' 2.0~ lr.'tO lt*'51 l~-~5 2l-~f6 lll.l1- IS"J5Ct Date: n5 .l~dl

   'I C = Larvae weight (mg) - B- A
                                                                                                    ~.~'(                        ,."(~      \.iS       ,.aa     \.U..       t.c..t Hand calculated.AI Analyst:                        "'       ~

S,llo 1.wo "1.1~ (1. "2.'(

                                                                                                             "'*~       "'-~~

_I Weight per Initial number of larvae (mg)

         = C I Initial number of larvae
                                                                            ~0                "'~     ~,ol.. .l_t>       ~~~        ~..),. v,_,         'fa\      ,..,,    '-\

c;~~ /::-., \: I Hand calculated/\) i'\ '\ \ Analyst: 1~ ,_.

                                                               ~*
                                                                          ~

()" o*

                                                                                            \D

()' t;)' o* ()* ()* o* I)' o* o* A Average weight per initial Percent t{~:t.7. . C), \I.~ & \. '2.7.

                                                                                  'Z.O ,'\ 7.            o.~'"

number oflarvae (mg) reduction O.bifo I from control (%) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, I lg = unusually large, d&r = decanted and returned, w = wounded; Comments: I

  -I I    Page 82 of 100                                                                                          SOP AT21- Exhibit AT2l.l, revision 04-01-09

I.

  • Pimephales pramelas Chronic Refetence Toxicant Test EPA-821-R-02-0B, Method 1000.0 8 Quality Control u Verification of Data Entry, Calculations, a nd Statistical Analyses

-~

                                                                                          ..__...,. __ ,_                                                             ..... .. ---1>< _.,_

Test rwml>or: _ _ _ _ _ _ ___:P~pK::aat::::::_:mt:!;!~ ---IICI) CGntrcl Environmental Testing Solutions, Inc. A 8 10 10

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2230 22~ A* l 853

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                                                                                                         ---~~~--.,....

0.853 QB24 OJI67 6.3 0.&53 0.824 100.0 Test _ : _ _ __ _ _ _..,;M:::::il'f!.CI::!J.~1::0:!,,::2ll::1:!.1 OM7 ClQ 6.3

                                                                                                                                                                                                                                     -101 Net appllatH c          10        10           14.20                  23.!;7             9A1            0.947                                                   0.947 D          10        10           14.119                 22.51             8A2             0.842                                                   0.842 E          10        10           1453                   21.1i9             7-16           0.716                                                   0.716 F           10        9           13.74                  21.83             8.0!1           0.899                                                   0.809 450                                                                                                                     0.804              10.2                              97.5         0.78Z              7.l                 9.1 G           10       10           13.71                  2130               7.59           0.759                                                   0.75!1 H           10       10           14.10                  22.54             L44             0.844                                                   0.844 I           10        8           14.95                  21.24              &.29           0.786                                                   0 .629 J           10        8           14.41                  21.0S             6.64            0.130                                                   Q.664 600                                                                                                                     0.137               5.4                             85.0          0.713              ll.3               17.1 IC          10        9           B .99                  21.50             751             o..B34                                                  0.751 L           10        9           14.42                  22A8               8.06           0..8:96                                                 0.1106 M           10        9           14.85                  20.71             5.86            0.651                                                   0586 N           10        9           13.07                  20.ru              7.60           0..844                                                  0.760 750                                                                                                                     0.762              13.8                             9Q.O          0.6&6             13.1                20.9 0           10        9           14A2                   22-lS              7.73           0..859                                                  o.n3 p           10        9           14.44                  20.68             6.24            0.693                                                   0.624 Q           10        6           B56                    17.90              4.34           0.723                                                   OA34 R           10        6           14.48                  1851               4-.03          0.672                                                   0.403 900                                                                                                                    0.787              145                               6SJ)         051.9             29.9                40.2 s          10        6           13.92                  18.85              4.93           D.B22                                                   D.493 T           10        8           13.84                  21.28              7.44           0..930                                                  0.744 u           10        3           14.42                  16-17              1-75           0583                                                    D.17S v           10        3           13.71                  15.59              L88            0.627                                                   0.188 11150                                                                                                                   0.665              17.7                              5.0          0.163             17.6                ll.l w           10        2           15.45                  16.!;7             L22            0 .610                                                  0 .122 X          10        2           UJJS                   14.76              1.68           0.840                                                   0.168 Dun.-'sMSD ,.._         0.0764                MSO=                   Minimum Silnific:ont lliff-..ce I'MSD:                  a.a                   PMSO=                  ~* M"........ Siplificant Diffennc:e PMSO is* II>NSUOW oltat pt<Cision. The PMSO is the mbmum _ , clilltAnc. between tho oonuclond     _,tNt    can bededortd stotlslbly ..,..-_,,in *whole _ _, tOIIicity ...._
                                                                     .__.,.PMSObouodde-byUSEPA(10dl-tSo) =12%.

Upper PMSDbou>d deCermined byUSEPA(90th~)= 3""- lDw.. ondu_.-PMSObou>ds-..determil>edlromtho:lOdlond90dl.,.._u.,~. oiPMSOdotofromEPA'sW£Tin~V-blityStudy(USEPA,200~USU'A,2001b). File:: pptcb_OS0311.xlu Page 83 of 100 Eauad by: J. s....... O~t>blaln<dft-Aqaa002",Inc. Rovie>>'Od by: ~

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Statistical Analyses Larval Fish Growth and Swvival Teat*7 Da~ Sunival Start Date:. 51312011 Test ID: PpKC!CR SampleiD: REF*RefToxicant End Date: 5/1012011 Lab ID: ETS*Envir. Testing Sol. Sample Type: KCL-Potessium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP*Pimephalea promelas ~ Comments: Cone- I 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 0.9000 1.0000 1.0000 600 0.8000 0.8000 0.9000 0.9000 150 0.9000 0.9000 0.9000 0.9000 900 0.6000 0,6000 0,6000 0.8000 1050 0.3000 0.3000 0.2000 0.2000 Transfonn: Arcsin Sguare Root Rank  !-Tailed Number Total Cone*!!!~!!: Mean N*Mean Mean Min Max CV"A> N Sum Critical Re!J! Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 450 0.9150 0.9750 1.3713 1.2490 1.4120 5.942 4 16.00 10.00 40 0 600 0.8500 0.8500 1.1781 1.1071 1.2490 6.954 4 10.00 10.00 6 40 0 750 0.9000 0.9000 1.2490 1.2490 1.2490 0.000 4 10.00 10.00 4 40 0 900 0.6500 0.6500 0.9413 0.8861 1.1071 11.742 4 10.00 10.00 14 40 0 1050 0.2500 0.2500 0.5216 0.4636 0.5196 12.838 4 10.00 10.00 30 40 Auxilimy Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates nonnal distribution (p > 0.0 I) 0.94239873 0.884 0.46929573 0.8338!121

 ~ill: of variance cannot be confirmed HlJ!othesis Teat ~l:!!!!z O.OSl                     NOEC         LOEC           ChV          TU Steel's Many-Qne Rank Test                            450         600       519.615242 Treatments vs D-Control Trimmed Spearman*Ksrber Trim Level            EC50               95%CL 0.0%

5.0% 10.0% 20.0% Auco-15.0% 949.51 891.54 l0ll.l6l File: ppkclcr_0503ll.xlsx Entered by: J. Sumner Reviewed by:~

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Statistical Analyses Larvall'ish Orowth and Surviwl Test-7 Day Growth Start Date: 51312011 Tesi!D: PpKCICR Sample ID: RBI'-Ref Toxicant End Date: 5/1012011 Lab!D: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-BPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Cono- 2 4 D-Contto! 0.8530 0.8240 0.9470 0.8420 450 0.7160 0.8090 0.7S90 0.8440 600 0.6290 0.6640 0.1SIO 0.8060 1SO O.S860 0.7600 0.7730 0.6240 900 0.4340 0.4030 0.4930 0.7440 IOSO 0.!7SO 0.1880 0.1220 0.1680 Transform: Untransfonned  !-Tailed Isotonic ConC=!!!I!IL Mean N-Mean Mean Min Max CV% N t-Stat Criticel MSD Mean N-Mean D-Control 0.866S 1.0000 0.866S 0.8240 0.9470 6.34S 4 0.866S 1.0000

                *4SO        0.7820         0.9025        0.7820      0.7160         0.8440          7.180        4             2.1Sl            1.943       0.0764         0.7820        0.9025 600        0.7125         0.8223        0.7125      0.6290         0.8060         11.330        4                                                         0.712S        0.8223 7SO        0.68S8         0.7914        0.68S8      O.S860         0.7730         13.806        4                                                         0.68S8        0.7914 900        O.S18S         O.S984        0.5185      0,4030         0.7440         29.874        4                                                         O.S18S        O.S984 IOSO        0.!633        0.1884         0.1633      0.1220         0.1880         17.593        4                                                         0.1633        0.!884 Auxiliary Tests                                                                                               Slatistic                  Criticel                      Skew            Kurt Shapiro-Wilk's Test indicates nonnal distribution (p > 0.01)                                               0.929S9601                     0.749                    O.S40073SSJ -0.9741049S
!'-Test indicates equal variances (p = 0.97)                                                                1.04289341                47.46834S64 Hypothesis Test (1-lail. O.OS)                                                                                 MSDu        MSDp            MSB            MSB          F-Prob           df Homoscedastic I Test indicates significant differences                                                     0.0763S283   0.088!1637     0.014280S       0.00308783  0.07SOSI717          1,6 Treatments vs D-Conttol Linear Interpolation (200 Resamples)

Point ms/L SD 9S%CL(Bxp) Skew

!COS*                       230.72          96.49        103.78      614.32         0.9S07 ICIO                        4S4.64          81.89        211.64      691.81        -0.036S
!CIS                        S48.1S          86.S8        396.44      894.38         0.1S30 IC20                        708.22          87.46        443.8S      8S4.07        -0.1925 l*os IC40 782.17 898.74 SS.lS 32.73 523.40 790.71 935.70 961.7S
                                                                                   -0.7858!
                                                                                   -0.2823 ICSO                        936.00          22.79        8S8.25      984.19        -0.3662
  • indicates !C estimate less than the lowest concentration File: ppkclcr_050311.xlsx Entered by: J. Sumner Reviewed by:~

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Page 4 of5 I Species: Pimephales promelas PpKCICR Test Nwnber: ~ \ I I I CONTROL I ~ I I 450 mg KCVL I I 600 mg KCI/L I I 750 mg KCI/L I 900 mg KCI/L I I 1050 mg KCI/L I I STOCK I Page 86 of 100 SOP AT21 -Exhibit AT2l.l, revision 04-01-09

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Species: Pimephales promelas PpKCICR Test Number: ~\ CONTROL 750 mg KCIIL I, 900 mg KCI/L

'i t-1050 mg KCI/L

-I ~ I Page 87 of 100 SOP AT21 - Exhibit AT2l .l, revision 04-01-09

       .~

Ceriodaphnia dubia Chronic Reference Toxicant. Control Chart

       ~         Environmental Testing Solutions, Inc.

1.14 USEPA Control Limits(+/- 2 Standard Deviations) 1.12 1.10 . ******************* 1.08 1.06 1.04 1.02

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USEPA Warning and Control Limits (75 1h and 90 h Percentile CVs) c: 2.0

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c: 0.5 "0 I t-- 1.4 Laboratory Warning and Control Limits (1 dh and 2 51h Percentile CVs) 1.3 1.2 1.1 1.0 -**- ** - **- **- ** - ** - **-* *- ** - ** -* - ** - **- **- **- ** - ** - ** - ** - ** - **- **- **- ** 0.9 ************************************************* ***************************************** Test date

  • 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
  -            -    . Central Tendency (mean IC 25 )
  - * - .. - Warning Limits (mean IC 25 +/- SA. 10 or SA. 75 )
  *** *****
  • Control Limits (mean IC 25 +/- S A. 25, S A. 90, or 2 Standard Deviations)

Graphs eener.ued from assodattd excel spreadsheet. Excel spreadsheet I e d by: J. Sumner Page 88 of 1 00 Reviewed bv:

Ceriodaphnia dubia Chronic Reference Toxicant Control Cbart Environmental Testing Solutions, lnc. Stateud USEPA uboratory uboratory UStlPA US.EPA Test number Test date 7-day ICu CT s Control Limits S.uo Warning Limits S.u:s Control Limits S.._75 Warning Umits S00 Control Limits CV (giL NaCI) (giL NaCI) cr - zs cr + zs cr- s.._,. cr + s.._,. CT - Sus cr + SA.U CT- Sus CT + S.._n CT-S....,. CT+S....,. I 12.08-09 LOS 2 01.05- 10 1.08 1.07 0.02 1.02 1.11 0.09 0.98 LIS 0. 18 0.89 1.25 0.48 0.59 l. SS 0.66 0.4 1 1.73 0.02 3 02..()2- 10 1.07 1.07 0.02 1.04 LIO 0.09 0.98 Ll6 0. 18 0.89 1.25 0.48 O.S9 1.55 0.66 0.4 1 1.73 0.02 4 03.o2- 10 1.08 1.07 U.oJ 1.04 LIO 0.09 0.99 1.16 0. 18 0.89 125 0.48 059 l.SS 0.66 0.41 1.74 0.01 s 04.()6. 10 LOS 1.07 O.oJ 1.04 LIO 0.09 0.98 LIS 0.18 0.89 125 0.48 0.59 1.55 0.66 0.41 1.73 0.01 6 05 10 1.09 1.07 0.02 1.04 Lll 0.09 0.99 1.16 0.18 0.89 126 0.48 0.59 1.56 0.67 0.41 1.74 0.02 7 06-0&- 10 1.07 1.07 0.02 1.04 LIO 0.09 0.99 1.16 0.18 0.89 125 0.48 0.59 1.55 0.66 0.41 1.74 0.01 8 07 10 1.07 1.07 0.01 1.04 LIO 0.09 0.99 1.16 0 . 18 0.89 125 0.48 0.59 1.55 0.66 0.41 1.74 0.0 1 9 OS..OJ- 10 1.06 1.07 0.01 1.04 LIO 0.09 0.98 1.16 0. 18 0.89 12S 0.48 0.59 I.SS 0.66 0.41 1.73 0.01 10 09-14-1 0 1.07 1.07 O.oJ 1.04 1.10 0.09 0.98 1.16 0. 18 0.89 125 0.48 0.59 I.SS 0.66 0.4 1 1.73 0.01 II 10.05-10 1.08 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0. 18 0.89 125 0.48 0.59 1.55 0.66 0.41 1.73 0.01 12 10.05-10 1.08 1.07 O.oJ 1.05 LIO 0.09 0.99 1.16 0. 18 0.89 125 0.48 0.59 I.SS 0.66 0.41 1.74 0.0 1 13 ll.o2-10 1.09 1.07 0.01 1.05 LIO 0.09 0.99 1.16 0. 18 0.89 126 0.48 0.59 1.56 0.67 0 .41 1.74 0.01 14 12-07-10 1.06 1.07 0.01 I.OS 1.10 0.09 0.99 1.16 0 . 18 0.89 125 0.48 0.59 1.56 0.66 0.41 1.74 0.01 IS 01-1&.11 1.06 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0. 18 0.89 125 0.48 0.59 1.55 0.66 0.4 1 1.74 0.01 16 02.0&.11 1.06 1.07 0.01 1.05 1.10 0.09 0.99 Ll6 0. 18 0.89 125 0.48 O.S9 1.55 0.66 0.41 1.74 0.01 17 03.()&.11 1.05 1.07 0.01 1.04 LIO 0.09 0.98 Ll6 0. 18 0.89 125 0.48 0.59 1.55 0.66 0.41 1.73 0.01 18 04-05-11 1.06 1.07 O.oJ 1.04 1.10 0.09 0.98 1.16 0. 18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 19 04-05-11 1.07 1.07 0.01 1.04 1.09 0 .09 0.98 1.16 0.18 0.89 125 0 .48 0.59 1.55 0.66 0.41 1.73 0.0 1 20 05-03-1 1 1.07 1.07 0.01 LOS 1.09 0 .09 0.98 Ll6 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 O.DI N(}{e: 1.0 ICu ~ 7-day 25% inhibition concentration. An estimation of the concentration ofsodium chloride that would cause a 25% reduction in Cenoduphniu reproduction for the test population. CT ~ Central tendency (mean IC25). S - Standard deviation of the~ values. uboratory Control and Warning Limits l..aborruol)' control and warning limits wen: eslablisbed using the standard deviation of the I~ values corresponding to the I Oth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the teSt method and endpoinl s.._,. - Standard deviation corresponding to the 10"' percentile CV. (S.._ 10 = 0.08) S.us = Standard deviation corresponding to the 25"' percentile CV. (SA25 - 0.17) USEPA Control and Warning Limits S.._7s ~ Standard deviation corresponding to the 75"' percentile CV. (S.._,5 = 0.45) S.._..= Standard deviation corresponding to the 90"' percentile CV. (S.._90 - 0.62) CV = Coefficient of variation of the I~ values. USEPA. 2000. Underslllnding and Accounting for Method Variability in Wbole Effluent Toxicity Applications Under the Natiooal Pollutant Discharge Elimination l'rogJam. EPA-833-R..OO..OOJ. US Environmental Protection A&ency. Cincinnati, OH. Page 89 of 100 File:: CdNit....."'l_OiOJ IJ..ds.x

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u= c! 50 USEPA Upper PMSD Bound (90th percentile< 47.0%) 40 30 20 10 Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD)

   ......... Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)

Graphs generated from associated excel spreadsheet. Page 90 of 100 Excel spreadshee~tered by: J. Sumner Reviewed by:

 **                                                                      Precision of Endpoint Measurements
~

Ceriodaphnia dubia Chronic Reference Toxicant Data

~      Environmental Testing Solutions, Inc.

Test Control Control Mean number Test date Surviva l Reproduction CT cv CT MSD PMSD CT for Control Mean for Control (%) (offspring/female) Reproduction (%) Reproduction (%) for PMSD (%) (offspring/female) CV(%) 1 12-08-09 100 35.2 4.6 2.0 5.8 2 01-05-10 100 31.1 33.2 5.8 5.2 2.3 7.3 6.6 3 02-02-10 100 31.9 32.7 3.5 4.6 2.6 8.3 7.1 4 03-02-10 100 31.0 32.3 6.3 5.0 2.4 7.7 7.3 5 04-06-10 100 32.7 32.4 6.3 5.3 2.4 7.4 7.3 6 05-04-10 100 31.0 32.2 4.6 5.2 2.3 7.4 7.3 7 06-08-10 100 33.1 32.3 6.6 5.4 2.4 7.2 7.3 8 07 10 100 31.6 32.2 4.8 5.3 2.3 7.2 7.3 9 08-03-10 100 28.2 31.8 4.7 5.2 1.8 6.2 7.2 10 09-14-10 100 32.9 31.9 8.7 5.6 3.0 9.2 7.4 11 10-05-10 100 30.2 31.7 7.6 5.7 2.0 6.6 7.3 12 10 10 100 30.8 31.6 4.0 5.6 2.2 7.2 7.3 13 11-02-10 100 30.5 31.6 4.9 5.6 2.0 6.6 7.2 14 12-07-10 100 32.2 31.6 4.8 5.5 2.0 6.2 7.2 15 01-18-11 100 31.9 31.6 6.3 5.6 2.3 7.1 7.2 16 02-08-11 100 30.7 31.6 6.9 5.6 1.8 5.7 7.1 17 03-08-11 100 32.6 31.6 4.8 5.6 2.0 6.0 7.0 18 04-05-11 100 32.8 31.7 5.9 5.6 2.2 6.8 7.0 19 04-05-11 100 33.0 31.8 4.5 5.6 1.9 5.7 6.9 20 05-03-11 100 30.9 31.7 6.7 5.6 2.5 8.1 7.0 Note: CV = Coefficient of variation for control reproduction. Lower CV bound determined by US EPA (JOih percentile)= 8.9%. Upper CV bound determined by US EPA (901h percentile)= 42% MS D = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (IOih percentile) = 13%. Upper PMSD bound determined by USEPA (901h percentile)= 47%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200Ja, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. File: CdNaC1CR_050311.xlsx Entered by: J. S~er Page 91 of 100 Reviewed by:~

Page I of6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaohnia dubia CdNaCICR#: Dilution preparation informadon: Comments: NaCI Stock INSS number: *~ '\~.,

       .Stock preparation:                       100 g NaCIIL:

Dissolve 50 g NaCI in 500 mL Milli*O water. Dilution prep (mg/L) 600 800 1000 ' 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test Ol'l!anism source in,{ormation: Test information: Qrganism age: < 24-hours old Randomizing template color: -c:Hd...h Date and times organisms were born between: os-.t\ CJ'\\,. TC> \OZ.OO Incubator number and shelf Culture board: t1-1.*1..S..*I\ A location: '2.& \ Replicate number: l 2J3J4JSI6J718 1 9 1 10 YWTbatch: Culture board cup number: 1.\ S I i I 1\ I 1~ I I~ I \~ I I.t 11...\1~ o&.\o '1.\*1 \ Transfer vessel information: oH-=t.ao s.u. Tempe_rature =- 'l.o.&.'\ oc Selenastrum batch: o4t'1*& \ Average transfer volume (mL): ~.~..t,<.-s! Daily renewal information: Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 2 3 4 5 6 7 Control information: Acceptance criteria Summa_I'J'_ o.{Jest end]J()ints:

      %of Male Adults:                                   67..                       ~20%                 7-day_LCso            ) Nl'l()
      %Adults having 3ra Broods:                        /ttl7,                      ~80%                 NOEC                    &oo
      % Mortality:                                        {')7.                     ~20%                 LOEC                   /OM Mean Offspring/Female:                            ~.Cl               ~ I5. 0 offspring/female      ChV                   gttc.t.t/
      %CV:                                               ~.17...                   <40.0%                                      10_~~- (a

~ 1 ICzs Page 92 of 100 SOP AT14 - Exhibit ATl4.l, revision 04-0 l-09 l; 1

Page2 of6 I ~ Species: Ceriodaphnia duhia CONTROL Day 1 2 3 Survival and Reproduction Data 4 Replicate number s 6 7 CdNaCICR #: 8 9 l'J-0 10 a_

    'I 1        Young produced           0          0          _C            0             0               Q_            0           0                   ()

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() () () L t) L () L tJ L. 0 L-0 L.. 7 Young produced \&. \5 \~ '~ \~ \~ l~ \~ 1\o tS I Total young produced

                                                   ~lo       4i            ~?} 32.                   ?,.C.,       '!J~          ~\            3() ~\ ~\

Final Adult Mortality \...... '- L L l l \. L L.. '-- I Note: Adult mortality (L- hve, D- dead), SB =split brood (smgle brood spht between two days), CO- carry over (offspnng carried over with adult during transfer). Concentration:

  ~I
                                                                                                                 % Mortality_:                                   07.

Mean Offspring/Female: ~o.i

                                                                                                                 % Reduction from Control:                      o.a?.

Page 93 of 100 SOP AT 14 - Exhibit AT 14.1, revision 04-01-09

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Day 1 l 3 4 Replicate number 5 6 7 8 9 10 I I Young produced Adult mortality 0 L 0

                                                                         \..-

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(\ 6 L.. 0 L _a 0 L L 0 0 L... c\._ 2 Young produced 0 0 0 () Q a 0 0 c I' Adult mortality t.. L L \... '-- '- L L L '- 3 Young produced 0 6 0 c 0 0 c 0 0 0 Adult mortality

                                                            '-          L        '--          '-           L          '-       '--         '-- '---                     '-

4 Young produced ~ '-\ '-\ s 4 '--l ..!. 3 s Adult mortality Young produced

                                                            \"l..      \0
                                                                         '-      \.......*

10 H lO

                                                                                                                 \.

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                                                                                                                              \0
                                                                                                                                \.....      '--

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                                                                                                                                               "'           \..-

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                                                                                                                                                                       ~~

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                                                                                 ~            c
                                                                                               '-          L

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                                                             \.
                                                            \'5 \C::.

L L

                                                                               \"'\
                                                                                             \0::,         l~
                                                                                                               \._

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                                                                                                                                          ,,  '- L '--

1'-t 15 Total young produced

?>\ '2.& ~\ 3() 2& ~'2.. ~ ~\ '2., 3\

I Final Adult Mortality \.... '-- ~ \....... '- \ '-- Note: Adult mortality (L- hve, D- dead), SB = spht brood (smgle brood spht b~tween two days), CO= carry over (offspnng carried over with adult during transfer).

                                                                                                                                               \.......    \....     '-

Concentration: I  % Mortality: Mean Offspring/Female: 07..

                                                                                                                                                                ..2!t5_
                                                                                                                  % Reduction from Control:                      a:z.1.

I 1000 mg NCI/L a S urvwa . I an dReproduction Dat,a ReDiicate number . Day 1 2 3 4 s 6 7 8 9 10 I 1 Young produced Adult mortality G L L (\ L (i L ()

                                                                                                        \.._.

1'1 L () L () () L L 0 () L-2 Young produced c 0 c 0 (") a 0 Q 0 0 I 3 Adult mortality Young produced Adult mortality ('} L

                                                                        \._

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   -I                                                      ¢          ~                     '-l                              3            s               3          3 4         Young produced                                                           ~          ~

s Adult mortlllity Young produced L 10 L 11..

                                                                              ""\.....
                                                                                  ~

L'l.. L It) L.

                                                                                                                       /0 L

ct II IC L L 10 Adult mortality L \.._ L L L L L L L L 6 Young produced Q 0 G () () (') 0 (\ 0 0 Adult mortality \ L L L \ L- L L L- '-- I 7 Young produced Total young produced

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J~tTISUngSolutlons.lnt. Species: Ceriodaohnia duhia CdNaCICR #: ll.O 1200 mtt Na CI/L S UTVIV. aJ andRepro uctzon Data

   '~                Day 2

l Young produced Adult mortaUty Young produced Adult mortallty 1 () D L- L. 0 C) 2 Q 0_ 3 0 L.. (') 4 Replicate number L

                                                                                                   ~

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                                                                                                                                    \l        ~             18.

Final Adult Mortality '- '- '- '- '- L ~ Note: Adult mortal1ty (L- live, D- dead), SB =split brood (smgle brood split between two days), CO- carry over (offspnng carried over with adult during transfer).

  • Concentration:
                                                                                                           % Mortality:                               07.

Mean Offspring/Female:

                                                                                                                                                  *~*~

I 1400 mtt NCIIL Day_ a 1 2 3 S urvzva 4 5

                                                                                                          % Reduction from Control:
                                                                                          . I andReproduct'wn D ata Reulicate number 7           8        9 S1.0f.

10

    ~

6 1 Young produced (") 0 0 (\ () () 0 0 Q 0

    ,r Adult mortality            \....   \....      \....    \._       L.            L         \...... L        L            L...

2 Young produced 0 0 0 0 0 6 0 0 0 0

    ~

Adult mortafity L L L L. L '- '-- L.. \..._ L.. 3 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L.. L. L. \.... L L.. '- L L c

   *I 0
                                                                                          '         0 4        Young produced              I      ("}      D                                                        7... 0              0 Adult mortlrlity          \.....     \..... '-          '-         L-          '-       '-           \....   \..........    \....:

s Young produced 0 '2. \ 0 "L l.. () 0 \ ~- Adult mortality L.. L. \.... L L.. L L L L l 6 Young produced .3_ 0 c 0 (') 0 ..3 0 0 tJ Adult mortality _\....... L L. L \.._ L L., ~ L L. I 7 Young produced Total young produced

                                                         '-\

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3 (o F1nal Adult Mortality '- L L L L \..... L \....... I Note: Adult mortality (L =live, D =dead), SB -split brood (smgle brood split between two days), CO- carry over (oftspnng carried over with adult during transfer). I Page 95 of 100 SOP AT 14 - Exhibit AT 14.1, revision 04-01-09

                                                                                                    ),                              ....... :  '  . -   ' ,_.......,.__,,_". ~;_.._ .... ~.
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_) Verification of Ceriodaphnia Reproduction Totals

  • J Environmental Testing Solutions, Inc.

Control 1000 mg NaCIIL Replicate number Replicate number Day Total Day Total I 2 3 4 s 6 7 8 9 10 1 2 3 4 s 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 3 5 4 4 6 4 4 3 41 4 3 3 4 4 3 3 3 5 3 3 34 5 12 12 10 13 11 12 12 12 12 10 II6 s 10 12 9 12 10 10 9 II 10 10 103 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 16 13 14 16 16 15 16 13 17 152 7 16 15 13 13 15 14 14 14 15 14 143 Total 32 32 26 32 31 32 33 32 29 30 309 Total 29 30 26 29 28 27 26 30 28 27 280 600 mg NaCIIL 1200 mg NaCIIL Replieate number Replicate number Day Total Day Total I 2 3 4 5 6 7 8 9 10 I 2 3 4 s 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 3 3 4 3 4 5 4 4 4 39 4 2 3 3 4 3 I 3 2 2 4 27 5 13 10 12 12 10 12 II II II 12 114 5 8 5 5 7 4 6 4 5 4 7 55 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 18 15 15 16 13 17 15 15 16 15 !55 7 9 8 6 3 5 0 6 4 3 7 51 Total 36 28 30 32 26 33 31 30 31 31 308 Total 19 16 14 14 12 7 13 II 9 18 133 800 mg NaCIIL 1400 mg NaCI/L Replieate number Replieate number Day Total Day Total I 2 3 4 5 6 7 8 9 10 1 2 3 4 s 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 3 4 4 5 4 4 4 3 3 38 4 I 0 0 I 0 0 0 2 0 0 4 s 12 10 10 II 10 12 10 10 10 l3 108 5 0 2 I 0 2 2 0 0 I 3 II 6 0 0 0 0 0 0 0 0 0 0 0 6 3 0 0 0 0 0 3 0 0 0 6 7 15 15 17 15 13 16 16 17 14 15 153 7 4 I 0 4 2 2 I 5 0 3 22 Total 31 28 31 30 28 32 30 31 27 31 299 Total 8 3 1 5 4 4 4 7 I 6 43 File: CdNaCICR 050311.xlsx Page 96 of 100 Entered by: J. Sumner Reviewed by: cJ

***                                   r                             Ceriodaplmia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0
                                      ~
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  ~   Environmental Testing Solutions, Inc.

Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test n11mbu: CdNaOCR IH20 Test dates: May 03-10, 2011 Concentration Replicate number Sunivai Average reproduction Codfociartor Ptrc:eat red11ctioa fro

  • nriatioa (%) oootrol ('Yo)

(mg/LNaCI) I 2 3 4 5 6 7 8 9 10 W*l (offspring/female) Control 32 32 26 32 31 32 33 32 29 30 100 30.9 6.7 Not a pplicable 600 36 28 30 32 26 33 31 30 31 31 100 30..8 8..8 0.3 800 31 28 31 30 28 32 30 31 21 31 100 29.9 5.6 3.2 1000 29 30 26 29 28 21 26 30 28 27 100 28.0 5.3 9.4 1200 19 16 14 14 12 7 13 II 9 18 100 13.3 28.4 57.0 1400 8 3 I 5 4 4 4 7 I 6 100 4.3 53.8 86.1 Dunnett's MSD value: 2.511 MSD = Minimum Significant Difference PMSD: 8.1 PMSD = Peroent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum pcroent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lowcr- PMSD b9und determined by USEPA (10111 percentile) = 13%. Upper PMSD bound determined by US EPA (90tb percentile) = 47%. Lower and upper PMSD bounds were determined from the l Oth and 90th percentile, respectively. ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA., 2001a; USEPA., 200 Ib). USEPA 2001a, 2001b. Final Rcpon: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods. Volumes l and 2-Appcndix. EPA-821-B-01-004 and EPA-82 1-B-01-005. US Environmental PrOicction Agency. Cincinnati, OH. File: CdNaCICR_050311 .XIsx Table populated from associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheet. Spreadsheet entered by: J . ~ner Reviewed by: Page 97 of 100

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                                              .....~,,,-p:-.:-;y Statistical Analyses Cerioda2hnia Survival and Reeroduction Test-RS!roduction Start Date: 5/3/2011                                           TestiD:       CdNaCICR                                 SampleiD:                 REF-RefToxicant End Date:                  5/10/2011                           Lab ID:       ETS-Envir. Testing Sol.                  Sample Type:              NACL-Sodium chloride Sample Date:                                                   Protocol:     FWCHR-EPA-821-R-02-013                   Test Species:             CD-Ceriodaphnia dubia Comments:

Conc-m~L 2 3 4 5 6 7 8 9 10 D-Control 32.000 32.000 26.000 32.000 31.000 32.000 33.000 32.000 29.000 30.000 600 36.000 28.000 30.000 32.000 26.000 33.000 31.000 30.000 31.000 31.000 800 31.000 28.000 31.000 30.000 28.000 32.000 30.000 31.000 27.000 31.000 1000 29.000 30.000 26.000 29.000 28.000 27.000 26.000 30.000 28.000 27.000 1200 19.000 16.000 14.000 14.000 12.000 7.000 13.000 11.000 9.000 18.000 1400 8.000 3.000 1.000 5.000 4.000 4.000 4.000 7.000 1.000 6.000 Transform: Untransformed  !-Tailed Isotonic Conc-mg!L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 30.900 1.0000 30.900 26.000 33.000 6.728 10 '30.900 1.0000 600 30.800 0.9968 30.800 26.000 36.000 8.766 10 0.091 2.287 2.511 30.800 0.9968 800 29.900 0.9676 29.900 27.000 32.000 5.563 10 0.911 2.287 2.511 29.900 0.9676

            *1000                    28.000               0.9061        28.000       26.000        30.000          5.324       10            2.641         2.287      2.511        28.000        0.9061
            *1200                    13.300               0.4304        13.300         7.000       19.000        28.366        10           16.027         2.287      2.511        13.300        0.4304
            *1400                       4.300             0.1392          4.300        1.000         8.000        53.763       10          24.223          2.287      2.511         4.300        0.1392 Auxiliary Tests                                                                                                          Statistic                 Critical                  Skew           Kurt Kolmogorov D Test indicates normal distribution (p > 0.01)                                                             0.82175648                   1.035                 -0.1981616 0.65572311 Bartlett's Test indicates egual variances <2 = 0.07)                                                                   10.1990776                15.0863171 Hypothesis Test (1-tail, 0.05)                                    NOEC         LOEC           ChV            TU           MSDu        MSDp          MSB         MSE         F-Prob           df Dunnett's Test                                                      800         1000       894.427191                  2.51109516 0.08126522 1279.06667 6.02962963          3.3E-34         5, 54 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point mg/L SD 95%CL Skew IC05 857.368421 101.848477 536.115476 1000.42718 -1.5395 ICIO 1002.58503 37.7430109 902.799145 1019.00076 -1.1222 ICI5 1023.60544 9.16420366 1004.92346 1039.04671 0.1840 IC20 1044.62585 8.95991641 1027.21063 1060.90996 0.1797 IIC25 1065.64626 9.15526908 1047.78147 1082.77122 0.19211 1C40 1128.70748 11.7423916 1107.58171 1149.34211 0.4319 IC50 1170.7483 14.442622 1144.89854 1199.51128 0.5658 File: CdNaCICR 0503Il.xlsx Entered by: J. Swnner Reviewed b y : + Page 98 of 100

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Page 5 of6 l I

    .J t-1~nl*l'fu&lnt5ohlloi'I-.MC.

II Species: Ceriodaphnia dubia CdNaCICR #: \2..0 I I I CONTROL I I I 600 mg NaCVL I I 800 mg NaCVL I I I I I I ~ I STOCK I Page 99 of 100 SOP ATl4- Exhibit ATl4.1, revision 04-01-09

Page 6 of6 Species: Ceriodaphnia dubia CdNaCICR #: \ 'Z.O I CONTROL ~ I ~--------~~------~~--~~~~~P---~~~--~~~~----~~~~~~~~ I 600 mg NaCI/L l~~~~~~~~~~~~~~p~ i 800 mg NaCI/L 1~---~~~~~~~~~~~~~~~~~~~~ I 1000 mg NaCI/L ~~~L-J..!::.=:..:__.

  ~~---~~---~~*~~~~~~~~~~---~~~

I I I

~ I I          Page 100 of 100                  SOP AT14 - Exhibit ATI 4.1, revision 04-0 I-09

PERMITTEE NAME/ADDRESS (Include Facility Name/location if Different) NATIONAl POUUTANT DISCHARGE ELIMINATION SYSTEM (NPOE$) MAJOR Form Approved. DISCHARGE MONITORING REPORT (OMR) Nam~ -W~SEOU~~~UC~ARP~~ --- (SUBR 01) OMB No. 2040-0004 Address_p...Q..JlOX.~ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ CINTEROFFICE OPS*SN-SQNI _ _ _ _ _ _ _ -- - ~ODDY~AI~fflinM_ _ _ _ _ _ _ _ Facili~ _WA~EQ~~~UClEARP~NC _ _ _ _

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I 7~~ NAME/TinE PRINCIPAL EXECUTIVE OFFICER I Certify uncles penally or law lhal this doa.ment and all attadlments wem prepared uncles my TELEPHONE DATE direction oc supervision in acoordance with a system desig1ed to assure that qualified personnel Michael D. Skaggs property galher and evaluate the infonnalion S<bmitted. Based on my inquiry or the person oc persons ..t1o manage the system, a< those persons directly responsille fa< gathering the Sequoyah Site ViJ Zident information, the information submitted is

  • to the best or my knowledge and belief, true, acrurate, 423 843-7001 11 06 07 Sequoyah Site Vice President and canplete. I am aware lhat there are significant penalties roc sU:Jmitting false infonnation, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility or fine a nd imprisonment for J<no..;ng viola lions.

TIPED OR PRINTED OFFICER OR AUTHORIZED AGENT

                                                                                                                                                                                                     ~~ I       NUMBER          YEAR      MO    DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS              (Reference all att3chments here EPA Fonn 3320-1 (REV 3199)        Previous editions may be used                                                                                                                                                             Page 1 of 1

PERMITIEE NAMEJADDRESS (lnclvde Facility NameA.ocation if Different) NATIONAL POLLUTANT DISCHARGE EUMINATION SYSTEM (NPDESJ MAJOR Fonn Approved. DISCHARGE MONITORING REPORT (OMR) Nam~-~~SEOU~A~UCLEAAP~~ --- (SUBR 01) OMB No. 204()..()()04 ACI<Jr~ _P ....Q*..!i..OX 2JXIQ_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ UNTERQE~UlES-~§.QID------ ---~ODDY~AI~TNi~--- -- --- Fadli~ _NA~~~~~L~RP~NC ___ _ EFFLUENT

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                                                                               ********               Req. Mon.                 MGD              ********                               ********                     ********
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                                                                                                                                                                                                                                   ~~~~ .1 OFFICER OR AUTHORIZED AGENT                                          NUMBER        YEAR      MO    DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS                     (Reference all attachments here No Discharge this Period EPA Fonn 3320-1 (REV 3199)               Previous editions may be vsed                                                                                                                                                                                 Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/toea/ion if Different) NATIONAl POU.UTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) ~mL_~~SEOUOYA~UCLEARP~ --- (SUBR 01 ) OMB No. 2040-0004 _M~~_p..Q.JtOX~ - _________ _ ---~ IN~R~~~PS*SN*S~ ------- ---~@DY~M~~~~-------- fi~~-~~~~~UCLEAR~NC _ _ _ _ EFFLUENT j-.Ltp:~~~:+--'l!M~ O ~QA~Y ~~io~A~N~NTI _________ _

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                                                                                                                                                                                   ..... NO DISCHARGE          I XX I .....

ATIN: stephanie A. Howard 1-=- 1 NOTE* Read instructions before complelill!llhis form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TIPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANAlYSIS IC25 STATRE 7DAY CHR SAMPLE MEASUREMENT

                                                                                                                             -                                       ********              ********            23 CERIODAPHNIA TRP3B     1   0     0                          PERMIT REQUIREMENT
                                                                        **"*****                    .........               -              43.2                      *******:I-            **"****'**      PERCENT                SEMI        COMPOS EFFLUENT GROSS VALUE                                                                                                                    MINIMUM                                                                                  ANNUAL IC25 STATRE 7DAY CHR                           SAMPLE MEASUREMENT
                                                                                                                             -                                       *******"'*            ********            23 PIMEPHALES lfRP6C    1    0 EFFLUENT GROSS VALUE 0                           PERMIT REQUIREMENT
                                                                        ********                     ********                -             43.2 MINIMUM
                                                                                                                                                                     ********              ........ . .. PERCENT                SEMI ANNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT I

L~2~v:P1L NAME/TlTLE PRINCIPAL EXECUTIVE OFFICER 1 Certify under penafty of law thai this docunent and all attaclvnenls were prepared under my TELEPHONE DATE direction 0< supeMsion in ac:ooroanoe with a syslem designed to assure that qualified personnel Michael D. Skaggs propelly gather and evaluate lhe inlonnation StbniUed. Based on my inq<My of the person or persons who manage the system, << those persoos dir~ responsible for gathering the

                                                .rlormation, the lllformation so.bnitted 1s
  • to the besl of my ~ and bebef, true, acrurate, 423 I 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that !here a1e signifocant penalties fO< s~bmi~Wlg false infonnabon, SIGNATURE OF PRINCIPAL EXECUTIVE inclucMg the possibility of IWle and n.>risonment for knowi1'1ll violations.

TIPED OR PRINTED OFFICER OR AlJTHORIZED AGENT

                                                                                                                                                                                                         ~~~ I      NUMBER         YEAR      MD    DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS               (Reference all attachments herE No Discharge this Period EPA Form 3320-1 {REV 3/99)         Previous editions may be used                                                                                                                                                             Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility N ame/location if Different) NATIONAL POLl UTANT DISCHARGE EliMINATION SYSTEM (N POES) MAJOR Fonn App roved. DISCHARGE MONITORING REPORT (DMR) NamL _~~SEQUOYA~UCL~RP~~- - - (SUBR 01 ) OMB No. 2040-0004 Address _p.O._!!_OX 2000_ _ _ _ _ _ _ _ _ _ _ _ 1--P-;-R-~-~T--N64 .u-

                                                                                                                             ; . M5~s0~ER---II J DISCHARG~ ~~M~ER I :~;;~ATER

_ _ _ _UNTEROFFICUlPS*SN*SQNl _ _ _ _ _ _ _ 02

 -- -~OD~~AI~TN~3M_ _ _ _ _ _ _ _                                                                                                                                                           & sToRM wATER Facili~ -~~EQUOYAHNUClEARP~NL _ _ _ _

w~io~AMIUONCOUNIT_ _ _ ______ _ EFFLUENT ATTN: stephanie A. Howard I YEAR I 05MO

                                                                                                                                                                            *"* NO DISCHARGE           IXX I *-

NOTE: Read instructions before complelill!l lhis form X PARAMETER QUANTITY OR lOADING QUAliTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYS1S OXYGEN, DISSOLVED 1 (DO) SAMPLE MEASUREMENT PERMIT

                                                                                                                                                                 ********         **'******           19 00300           0 REQUIREMENT
                                                                           "*******                  ********                                     2              ********         *******""          MGIL              TWICE/          GRAB EFFLUENT GROSS                                                                                                                             MINIMUM                                                                      WEEK SOLIDS, TOTAL SUSPENDED                         SAMPLE MEASUREMENT
                                                                           ********                  *'*******                  ...           '********          ********                             19

. 00530 1 0 PERMIT

                                                                                                                              ......          ********           ********           100              MG/L              TWICE/          GRAB REQUIREMENT EFFLUENT GROSS                                                                                                                                                                 DAILYMX                                  WEEK SOLIDS, SETTLEABLE                              SAMPl E MEASUREMENT 25 00545      1 EFFLUENT GROSS 0                               PERMIT REQUIREMENT
                                                                           ********                  1hl***'***               -               ********           ***'*****            1 DAILYMX MUL               ONCE/

MONTH GRAB FLOW, IN CONDUIT OR THRU SAMPlE **'****** ** ****** ******** MEASUREMENT 03 **

 !TREATMENT PLANT 50050     1    0                               PERMIT REQUIREMENT Req. Mon.                  Req. Mon.                 MGD               '********          ********         ********
                                                                                                                                                                                                       .                ONCE/         ESTIMA EFFLUENT GROSS                                                         MOAVG                    DAILYMX                                                                                                               BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
                                                                                                                                                           ~l~.~~~. .

NAMEmn.E PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law lhallhis document and all attac:lvnents were prepared under my TElEPHONE DATE

                                                  ~edion or supervision in aooordance      with a system desiglled to 3SSISe lha1 qualified personnel Michael D . Skaggs                proper1y gather and evaluate the i~ormation submitled. Based oo my inquiry of the person or
                                                  ~s who manage the system, or those persons d irectly responsible for gathering lhe information. the information submitted is , to the besl of my knowledge and belief. true, ~at.e ,                                             423       84~7001            11      06     07 Sequoyah Site Vice President             f.nd complete. I am aware lhallhere are signifocanl penalties for slbnW!mg false infonnatioo.         SIGNATURE OF PRINCIPAl EXECUTIVE               I including lhe possibility of fine and imprisonment for knowii1Q violations.
                                                                                                                                                                                                ~~~~ I OFFICER OR AUTHORIZED AGENT                     NUMBER         YEAR       MO    DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS              (Reference all attachments here During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

EPA Fonn 332G-1 (REV 3199) Previous editions may be used Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- May 2011 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 06/07/2011 CONCURRENCES Name R c Signature - Comment Date v N S.M. Love X S. A. Howard X D.A. Day X M.D. Skaggs X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction , and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 110711 800- NPDES CORRESPONDENCE July 11 , 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 1h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Dana Waits:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JUNE 2011 Enclosed is the June 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I cerUfy under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, ~.~~k(Jj Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 B. E. Brickhouse, LP 5U-C D. B. Nida, LP 5U-C G. M. Cook, OPS 4A-SQN A. A. Ray, WT 11A-K D. A. Day, POB 2A-SQN G. R. Signer, WT 6A-K S. A. Howard, OPS 5N-SQN M. D. Skaggs, OPS 4A-SQN K. Langdon, POB 2B-SQN K. M. Hodges (EDMS) LP 2V-C

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 July 11,2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Dana Waits:

SEQUOYAH NUCLEAR PLANT- DISCHARGE MONITORING REPORT FOR JUNE 2011 Enclosed is the June 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely,

~.J:t~~

Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Fonn Approved. DISCHARGE MONITORING REPORT (DMR) Nam~-N~SEOUOY~~UCLEAAP~~--- (SUBR 01) OMB No. 2040-0004 Addre...s_s_p....Q. BOX~------ _ _ _ _ _ ---~INTER~FIC~PS*SN*S~------- ---~~~AI~rn37~L ______ _ Facii~-~~EQU~AHN~LE~PLANL ___ _ EFFLUENT ~~~~~liT~~~---------- ATTN: stephanie A. Howard

                                                                                                                                                                             *** NO DISCHARGE            D      ***

NOTE: Read instructions before completinA this form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. CENTIGRADE SAMPLE MEASUREMENT

                                                                                                                         -                ********             ********               41.7               04        0      30/30        RCORDR 00010        1      0                         PERMIT REQUIREMENT
                                                                      ********                  ********                 -                ********             ********           Req.Mon.
                                                                                                                                                                               .. DAILY MAX DEG.C.              CONTI        CALCTD EFFLUENT GROSS                                                                                                                                                                                                             NUOUS TEMPERATURE, WATER DEG.                       SAMPLE                   ********                   ********                                 ********             ********               28.7                                30/30        MODELD MEASUREMENT                                                                                                                                                     04        0 CENTIGRADE 00010        z      0                         PERMIT                   ********                   ********                                 ********             ********               30.5            DEG.C.              CONTI        CALCTD REQUIREMENT INSTREAM MONITORING                                                                                                                                                                 DAILYMX                                NUOUS TEMP. DIFF. BETWEEN SAMP. &                   SAMPLE                    ********                  ********
                                                                                                                           -              ********             ********                 2                04        0      30/30        CALCTD UPSTRM DEG.C                              MEASUREMENT 00016        1      s                         PERMIT                   ********                   ********                                 ********             ********                 3             DEG.C.              CONTI        CALCTD REQUIREMENT EFFLUENT GROSS                                                                                                                                                                      DAILYMX                                NUOUS FLOW, IN CONDUIT OR THRU                      SAMPLE                    ********                   1688                                    ********             ********             ********                              30/30        RCORDR MEASUREMENT                                                                     03                                                                               **       0 TREATMENT PLANT 50050        1      0                         PERMIT                    ********               Req. Mon.                MGD                ********             ********             ********                               CONTI       RCORDR REQUIREMENT EFFLUENT GROSS                                                                               DAILY MAX                                                                                                                     NUOUS CHLORINE, TOTAL RESIDUAL 50060        1      0 SAMPLE MEASUREMENT PERMIT
                                                                                                                                                               ...Q. 825--.,..

o.o'Z..'f 0.1 1 9.968 0.031 0.1 1ie In 19 MG/l 0 -M"/30 1J:8.,/A/t FIVE PER CALCTD GRAB REQUIREMENT EFFLUENT GROSS MOAVC DAILY MAX WEEK TEMPERATURE- C, RATE OF SAMPLE ******** 1 ******** ******** 0 30/30 CALCTO MEASUREMENT 62 ** CHANGE 82234 1 0 PERMIT REQUIREMENT

                                                                       ********                      .2                DEG                 ********            ********              ******* .           ....              CONTI       CALCTD C/HR EFFLUENT GROSS                                                                                 DAILYMX                                               '                                                                     NUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT S::lh:..~~ai.m NAME/TinE PRINCIPAL EXECUTIVE OFFICER       I Certify under penalty of law that this document and all attachments were prepared under my                                                              TELEPHONE                   DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs            properly gather and evaluate the infonnalion submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President infonnation, the infonnalion submitted is , to the best. of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false infonnation, 423    I 843-7001         11       07     08 SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.

TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT

                                                                                                                                                                                                  ~~~~I       NUMBER        YEAR MO         DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No closed mode operation. Veliger monitoring data is in included as an attachment. The following injections occurred: 1. Towerbrom 960 2. Floguard MS6236 (max. calc. cone. was 0.11mg/L-Iimit 0.2mg/L) 3. Biodetergent 73551 (max. calc. cone. was 0.06mg/L-Iimit 2.0mg/L) 4. Spectrus CT1300 (max. calc. cone. was 0.038mg/L-Iimit 0.050mg/L)

EPA Fonn 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

Mean# of NOTES:% Mean# of Water Water SUB Sample Date %Settlers Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY ZM/m3 Temp.('C) Temp. ('C) LOCATION Clams/m3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-ISV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB 02/08/2011 0 0 9 02/08/2011 0 9 1-25-545 MJW 02/15/2011 0 0 23 02/15/2011 0 23 1-25-545 MLW 02/22/2011 20 100 10 02/22/2011 0 10 1-25-545 PB 03/01/2011 0 0 11 03/01/2011 0 11 1-ISV-24-1236 PB 03/08/2011 0 0 11 03/08/2011 0 11 1-ISV-24-1236 WE 03/16/2011 22 0 11 03/16/2011 0 11 1-ISV-24-1234 MLW 03/23/2011 0 0 11 03/23/2011 0 11 1-ISV-24-1234 MLW 03/30/2011 0 0 12 03/30/2011 0 12 1-15v-24-1236 MLW 04/06/2011 18 100 15 04/06/2011 0 15 1-ISV-24-1234 HMW .04/08/2011 45 100 15.5 04/08/2011 0 15.5 1-1SV-24-1236 WAW/PB 04/20/2011 21 100 16 04/20/2011 0 16 1-1SV-24-1236 PB May 2011 No Samples Collected June 2011 No Samples Collected

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved. MAJOR DISCHARGE MONITORING REPORT (DMR) Nam~-~~SEOU~~~UCLEARP~~--- (SUBR 01} OMB No. 2040-0004 Address J....Q.JtOX 2000_ _ _ _ _ _ _ _ _ _ _ _ ---~INTER~FICEOPS-5N2~------- ---~~~AI~m~~-------- Facii~_WA~EQUO~HN~~ARP~NC ___ _ EFFLUENT ~~~~AMI~~~----------

                                                                                                                                                                                 *** NO DISCHARGE           D      ***

ATTN: stephanie A. Howard NOTE: Read instructions before completing this form. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ******** ******** ** Monitoring ******** ******** 23 CERIODAPHNIA MEASUREMENT Not Required TRP38 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT

                                                                              ********                    ********              -                   43.2*

MINIMUM

                                                                                                                                                                      ********         ********        PERCENT               SEMI ANNUAL COMPOS IC25 STATRE 7DAY CHR                                  SAMPLE                   ********                    ********                             Monitoring             ********         ********

MEASUREMENT ** 23 PIMEPHALES TRP6C 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT

                                                                              ********                    ********              -            Not Required 43.2 MIMINUM
                                                                                                                                                                      ********         ********        PERCENT               SEMI ANNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my                                                                      TELEPHONE                   DATE Vi~ident direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs                       properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site                         423       843-7001 information, the information submitted is , to the best of my knowledge and belief, trua, accurate, 1----,-----,------,---------1                                        11       07    08 Sequoyah Site Vice President                   and complete. I am aware that !hera are significant penalties for submitting falsa information,       SIGNATURE OF PRINCIPAL EXECUTIVE 1 - - - - - - - - - - - - - - - - - - - j i n c l u d i n g the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXP~NATION OF ANY VIO~TIONS £Reference all attachments here Toxicity was not sampled in June 2011. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Fonn Approved. MAJOR DISCHARGE MONITORING REPORT (DMRJ NamL_N~SEOU~~~UCLEAAP~~--- (SUBR 01) OMB No. 2040-0004 Address _p..Q. BOX 2.._ _ _ _ _ _ _ _ _ _ _ _ ---~IN~~~~~-SN*S~------- ---~ODm~AI~W~~-------- Fa~~-~A~~UmAHNg~RP~NL _ _ _ _ EFFLUENT ~~~~~T~COUN~----------

                                                                                                                                                                        *** NO DISCHARGE           D      ***

ATTN: stephanie A. Howard NOTE: Read instructions before completing this fonn. X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS SAMPLE ******** ******** 7 ******** 8 13/30 GRAB PH MEASUREMENT ** 12 0 00400 1 0 PERMIT ******** ******** ** 8 ******** 9 su THREE/ GRAB REQUIREMENT EFFLUENT GROSS MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** 6 8 0 2/30 GRAB MEASUREMENT ** 19 00530 1 0 PERMIT ******** ******** ** ******** 30 100 MGIL lWICEJ GRAB REQUIREMENT EFFLUENT GROSS MOAvo* DAILYMX- MONTH OIL AND GREASE SAMPLE ******** ******** ******** <6 <6 0 2/30 GRAB MEASUREMENT ** 19 00556 1 0 PERMIT ******** ******** ** ******** 15 20 MGIL lWICEJ GRAB REQUIREMENT EFFLUENT GROSS MOAVO DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.044 1.186 ******** ******** ******** RCORDR 03 ** 0 30/30 TREATMENT PLANT 50050 1 0 MEASUREMENT PERMIT REQUIREMENT Req. Mon. Req. Mon MGD ******** ******** ******** . SEE RCORDR EFFLUENT GROSS MOAVO DAILYMX PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT (~'if.. ~...... NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those parsons directly responsible for gathering the information, tha information submitted is , to the best of my knowledga and beliaf, true, accurate, 423 843-7001 11 07 08 Sequoyah Site Vice President and complate. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of line and imprisonment for knowing violations.

             ~PED  OR PRINTED OFFICER OR AUTHORIZED AGENT
                                                                                                                                                                                            ~~~~I      NUMBER        YEAR      MO    DAY COMMENTS AND    EXP~NATION   OF ANY VIOLATIONS         £Reference all attachments here EPA Form 3320-1 (REV 3/99)        Previous editions may be used                                                                                                                                                   Page 1 of 1

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) Name TVA__.:_ SEOUOYAH~UCLEAR PLAN!_ _ _ _ (SUBR 01) OMB No. 2040-0004 Addre~ P...Q.BOX200Q_ _ _ _ _ _ _ _ _ _ _ _ ---~INTEROFFICE0~*5N*S~------ _ _ _ JOD~~AI~rn~3~-------- F~lii~-~~~~~HN~LEARP~N_ _ _ _ _ ~c~o~AMIIT~~OUNn _________ _ EFFLUENT

                                                                                                                                                                             * - NO DISCHARGE           IXX I .-

ATTN: stephanie A. Howard NOTE* Read instructions before completing this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRAnON NO. FREQUENCY SAMPLE EX OF ITPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ** ******** ******** 04 MEASUREMENT CENTIGRADE 00010 1 0 PERMIT ********* ******** ** ******** ******** REPORT DEGC CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE DAILYMX uous TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** MEASUREMENT ** 04 CENTIGRADE 00010 z 0 INSTREAM MONITORING PERMIT REQUIREMENT

                                                                       ********                   ********                -               ********           "'"'***"'"'*            30.5 DAILYMX DEGC              CONTIN.

uous CALCTD jrEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** MEASUREMENT ** 04 UPSTRM DEG.C 00016 1 0 EFFLUENT GROSS VALUE PERMIT REQUIREMENT

                                                                       *****"'**                  *"'"'"'**"'*            -               *******"'          ****"'***                 5 DAILYMX DEGC              CONTIN uous CALCTD FLOW, IN CONDUIT OR THRU                     SAMPLE                    ********                                                            ********          ********              ********

MEASUREMENT 03 ** TREATMENT PLANT 50050 1 0 PERMIT **"'****"' Req. Mon. MGD ******** "'***"'*** ******"'* "* CONTIN RCORDR REQUIREMENT EFFLUENT GROSS VALUE DAILYMX uous CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT

                                                                                                                          -                ********                                                     19 50060     1     0 EFFLUENT GROSS VALUE PERMIT REQUIREMENT MOAVC 0.1                  0.1 DAILYMX MGIL              Five per.

Week CALCTD TEMPERATURE- C, RATE OF SAMPLE ******** ******** ******** ******** MEASUREMENT 04 ** CHANGE 82234 1 0 PERMIT ******** 2 DEGC ******** ******"'* ******** "* CONTIN CALCTD REQUIREMENT EFFLUENT GROSS VALUE DAILYMX uous SAMPLE MEASUREMENT PERMIT REQUIREMENT I

                                                                                                                                                       ...,.,..~:.!.:~.

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of Jaw that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 I 843-7001 11 07 08 and complete. 1 am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECunVE including the possibility of fine and imprisonment for knowing violations. nPED OR PRINTED OFFICER OR AUTHORIZED AGENT

                                                                                                                                                                                                 ~~~I       NUMBER         YEAR     MO     DAY COMMENTS AND EXP~NAnON OF ANY VIO~nONS                 £Reference all attachments here No Discharge this Period EPA Fonn 3320*1 (REV 3/99)        Previous editions may be used                                                                                                                                                     Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility NameA.ocation if Different} NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES} MAJOR Fonn Approved. DISCHARGE MONITORING REPORT (DMR} NamL_~~SEOUO~~UCLEAAP~~--- (SUBR01) OMB No. 2040-0004 Address _p...Q.~OX 2Q9Q_ _ _ _ _ _ _ _ _ _ _ _ ---~lmEROF~EOPS~-S~------- _ _ _ _roDm~AI~ffl~SL _ _ _ _ _ _ _ Faciii~-~~EQU~AHNUCLEARP~NC _ _ _ _ r:rriNG p~:AWt EFFLUENT ~c~~~MI~NCN~---------- I MO DAY *** NO DISCHARGE IXX I *** ATTN: stephanie A. Howard NOTE: Read instructions before completing this fonn X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE MEASUREMENT

                                                                                                                           -                                 ********         ********            23 CERIODAPHNIA TRP3B     1    0    0                          PERMIT                    ********                   ********                                  43.2            ********         ********        PERCENT               SEMI        COMPOS REQUIREMENT MINIMUM EFFLUENT GROSS VALUE                                                                                                                                                                                               ANNUAL IC25 STATRE 7DAY CHR                           SAMPLE                     ********                  ********                                                  ********         ********

MEASUREMENT 23 PIMEPHALES TRP6C 1 0 0 PERMIT ******** ******** 43.2 ******** ********* PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT ... SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT r:J.j!~-* NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 11 07 08 Sequoyah Site Vice President and complete. I am aware that.there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE 1 including the possibility of fine and imprisonment for knowing violations.

            ~PED   OR PRINTED OFFICER OR AUTHORIZED AGENT
                                                                                                                                                                                            ~~~~I      NUMBER        YEAR      MO     DAY COMMENTS AND EXPLANATION OF ANY VIO~TIONS                 (Reference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3/99)          Previous editions may be used                                                                                                                                                Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameA..ocation if Different) . NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) NamL_W~SEOU~AA~UC~ARP~~--- (SUBR01) OMB No. 2040-0004 Address__p...Q.~OX ~ _ _ _ _ _ _ _ _ _ _ _ ---~INTEROF~EO~-SN~------ ___ J,~~AI~.m~~-------- Faciii~_WA~EQ@~~~~RP~NL ___ _

                                                                                                     ~-=~P. .; ;.;.R~.;,;~;.,; ~; ; ~;.; .~. ;,5;M . 0B;;..eR=-=~Il DISCHARG:   ~~M~ER :~~;~ATER I                   & sToRM wATER

~~lo~AMIOO~OUN~---------- MQ ~9RING ~ MQ DAY EFFLUENT ATTN: stephanie A. Howard From Iv~~R I 06 [ 01 ] To !11 J 06 30 *** NO DISCHARGE NOTE: Read instructions before completing this form X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF ~E AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE ******** ******** ******** ******** MEASUREMENT ** 19 00300 1 0 PERMIT ******** ******** **** 2 ******** ******** MG/l TWICE/ GRAB REQUIREMENT EFFLUENT GROSS MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT

                                                                                                                                            -                   ********          ********                              19 00530     1 EFFLUENT GROSS 0                                  PERMIT REQUIREMENT
                                                                           ********                  ********                             -                     ********          ********            100 DAILYMX MG/l              TWICE/

WEEK GRAB SOLIDS, SETTLEABLE SAMPLE MEASUREMENT PERMIT 25 MUL 00545 1 0 REQUIREMENT

                                                                                                                                                                                                       .1                                 ONCE/        GRAB EFFLUENT GROSS                                                                                                                                                                                     DAILYMX                                MONTH FLOW, IN CONDUIT OR THRU                          SAMPLE                                                                                                         ********          ********          ********

MEASUREMENT 03 ** TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD *****A:** ******** ********

  • ONCE/ ESTIMA REQUIREMENT EFFLUENT GROSS MOAVG DAILYMX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT L:J.~~"'

NAME/TinE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is

  • to the best of my knowledge and belief, true, accurate, 423 843-7001 11 07 08 Sequoyah Site Vice President and complete. I am aware thetthere are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.

SIGNATURE OF PRINCIPAL EXECUTIVE 1

                                                                                                                                                                                                                  ~~~~I OFFICER OR AUTHORIZED AGENT                      NUMBER        YEAR      MO   DAY
             ~PED   OR PRINTED COMMENTS AND EXP~NATION OF ANY VIO~TIONS (Reference all attachments here During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- June 2011 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 07/07/2011 CONCURRENCES Name R c Signature - Comment Date v N B.M. Love X S. A. Howard X D.A. Day X INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.}}