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Discharge Monitoring Report for December 2007
	ML080160592
Person / Time
Site:	Sequoyah
Issue date:	01/14/2008
From:	Cleary T; Tennessee Valley Authority
To:	Cromer P; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
References
	Download: ML080160592 (114)
	v • d • e

Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 January 14, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR DECEMBER 2007 Enclosed is the December 2007 Discharge Monitoring Report for Sequoyah Nuclear Plant.

Please contact me at (423) 843-6700 if you have any questions or comments.

Sincerely, Stephanie A. Howard Principal Environmental Engineer

.Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure

.cc (Enclosure):

Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Prircedon recycledpaper

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No: 2040-0004 Address P.O. BOX 2000 - (SUBR 01)

(INTEROFFICE SB-2A)

TN0026450 101 G__ F-FINAL SODDY - DAISY TN 37384

- PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY F MONITORING PERIOD EFFLUENT YEAR I MO I DAY I MO*DA ATTN: Stephanie A. Howard From 07 I12 I01 I.To 0 2 3 NO DISCHARGE NOTE: Read instructions before completinq this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCYL SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 4 4 4 4 04 0

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NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my , TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary Q L .

property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Pnncipal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, PrincipalEnvironmental_ 423 843-6700 08 01 14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA TYPED OR PRINTED NUMBER YEAR MO DAY CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REVEPA Form 3320-1 (REV 3/99) 3/99) Previous editions may Previouseditions may be be used used Page 1 of 2 Page 1 of 2

DMR Attachment CCW Data CCW TRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/13/2007 @ 1215 0.33 mg/L 12/16/07 @.1854 RDH TN EPA 8015 CCW CHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/13/2007 @ 1155 <0.10 mg/L 12/16/07 @ 1905 RDH TN EPA 8015

PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)

(SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000

- - L(NTEROFFICE SB-2A) TN0026450 101 G F-FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facility TVA - SEQUOYAH NUCLEAR PLANT I I Location HAMILTON COUNTY -- MONITORING PFRIODl I EFFLUENT ER MO DA YEAR MO DAY NO DISCHARGE ATTN: Stephanie A. Howard NOTE: Read instructions before completinp this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE 1~ -, -, -r ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 444 CHLORINE, TOTAL RESIDUAL SAMPLE GRAB MEASUREMENT 0.019 0.027 19 0 23/31 50060 1 0 0 MG/L WEEK- CALCTD, EFFLUENT GROSS VALUE 'REQUIREMENT MID AV INST MAX. DAYS TEMPERATURE - C, RATE OF SAMPLE 1 0 31131 CALCTD CHANGE MEASUREMENT 62 82234 1 0 .0 R P1 IT - - DEG * * *****

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SAMPLE MEASUREMENT NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Ceriify under penally of law that this document and all attachments were prepared under my , i i, . i l l TELEPHONE DATE

-directionor supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gatheringthe Principal Environmental Engineer 423 843-6700 08 01 14 Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, 423_843-6700_08_01_14 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE Sinformation, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

The following injections occured: 1. Biodetergent 73551 (max. calc. conc. was 0.022mg/L--limit 2.Omg/L)

EPA Form 3320-1 (REV 3/99) Previouseditions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUB R 01) OMB No. 2040-0004 Address P.O. BOX 2000 (INTEROFFICE SB-2A) TN0026450 101 Q F- FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBERE DIFFUSER DISCHARGE Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT YEART MO DAY I YEAR MO NO DISCHARGE ATTN: Stephanie A. Howard From 07 1 10 01 To 3 1 i nstrucH N21 ReNO t 11:1 NOTE: Read instruction!s before comoletinq this form.

PARAMETER I QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE I IANALYSIS MAXIMUM UNITS BORON, TOTAL <n 9nf n 1 /92 01022 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT REQUIREMENT SAMPLE MEASUREMENT EOU IRE MENNT NAMETITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE Timothy P. Cleary directin or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or

,47'J A 9~L)iX persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Sinformation, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 08 01 14 Site Vice President accurate, and'complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE A I N

_information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Boron was sampled on 10103/2007 @ 1328.

EPA Form 3320-1 (REV 3/99) . Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR For m Approved.

Name "TVA- SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUR 01) O lB No. 2040-0004 Address P.O. BOX 2000 (INTEROFFICE SB-2A) TN0026450 11 T F-FINAL SODDY- DAISY TN 37384 PERMITNUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT IYEAR MO DAY I YEAR MO_ý DAY NO DISCHARGE ATTN: Stephanie A. Howard From 07[ I 12 01 To 07 12 31 NOTE: Read instructions before completinq this form.

PARAMETER QU ANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF I TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS I- I- + I- I-IC25 STATRE 7DAY CHR CERIODAPHNIA SAMFPLE MEASUR EMENT *

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel .t 4AL[ "

Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer nformation, the information submitted is , to the best of my knowledge and belief, true. _._ _ _

Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICERr OR9 AU TIOTHORIZED AGEN-4T TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Toxicity was sampled 12/02/2007 - 1210712007. Report is attached.

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

°

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: Januaa 11 200 Facility / Discharger: Sequoyah Nuclear Plant /TVA

2. County /State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1579

6. Receiving Stream: Tennessee River (TRM 483.6)

7. 1Q10: 3491

8. OutfallTested: 101

9. Dates Sampled: December 2-7, 2007

10. Average Flow on Days Sampled (MGD): 1573, 1575, 1577, 1578, 1577, 1574

11. Pertinent Site Conditions: Towerbrom 960 was injected into ERCW A & B Trains and RCW. The dates and times for the Towerbrom 960 injection are in the following table.

See Appendix B for complete additional chemical application information during the sample collection period.

Injection Location(ET(S) Date/Start Time Date/Ending Time (EST) (EST)

ERCW A & B and RCW 12/02/2007 @ 0001 12/07/2007 2400

12. Test Dates: December 4-11, 2007

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephalespromelas)

Daphnids (Ceriodaphniadubia)

15. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0 Page 1 of 101

16. Permit Limit Endpoint (%): Outfall 101: IC = 45.2%

17. Test Results: Outfall 101: Pimephalespromelas:LC25 > 100%

Ceriodaphniadubia: IC 5 > 100%

UV treated Outfall 101: Pimephalespromelas:IC 2 5 > 100%

18. Facility

Contact:

Stephanie Howard Phone #: (423) 843-6700

19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Cynthia L: Russell Phone #: (256) 386-2755

22. Notes: Outfall 101 samples collected December 2-7, 2007, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >

100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant differences from the control during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.

Page 2 of 101

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101 ,Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM/DD/YY)/ (MM/DD/YY)/ Arrival Initial (MM/DD/YY)/

Sample Time (ET) Time (ET) Temp. TRC* Time (ET)

ID Collected Received (°C) (mg/L) Last Used By 101 12/02/07 0738 to 12/03/07 1434 2.7/3.1' <0.10 12/04/07 1250 12/03/07 0638 12/05/07 1156 12/02/07 0807 to 12/04/07 1250 Intake 12/03/07 0707 12/03/07 1434 2.4 <0.10 12/04/07.07527t 12/05/071156 12/06/07 158 12/05/07 1325 1 . 7 /1.2' <0.10 12/06/07 1158 101 12/05/070752 12/04/07 0652to 12/07/07 1155 12/04/07 0824 to .12/06/07 1158 12/05/07 1325 2.2 <0.10 12/07/07 1155 Intake 12/05/07 0724 12/05/07 0724 12/07/07 1155 12/08/07 1209 101 12/06/07 0750 to 12/07/07 1350 1 . 5 / 2 .0' <0.10 12/09/07 1311 12/07/07 0650 12/1.0/07 1300 12/08/07 1209.

Intake 12/06/07 0806 to 12/07/07 1350 0.4 <0.10 12/09/07 1311 12/07/07 0706 12/10/07 1300

TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 :L 1.0 C0 in a warm water bath.

Aliquots of Outfall 101 and Intake samples.were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Page 3 of 101

Pimephalespromelas Ceriodaphniadubia Test Organisms:

1. Source: Aquatox, Inc. In-house Cultures

2. Age: 20.75 hours8.680556e-4 days <br />0.0208 hours <br />1.240079e-4 weeks <br />2.85375e-5 months <br /> old <24-hours old Test Method Summary:

1. Test Conditions: Static, Renewal Static, Renewal

2. Test Duration: 7 days Until at least 60% of control females have 3 broods

3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic

4. Number of Replicates: 4 10 A

5. Organisms per Replicate: 10 I

6. Test Initiation: (Date/Time)

Outfall 101 12/04/07 1250 ET 12/04/07 1045 ET UV Treated Outfall 101 12/04/07 1237 ET

7. Test Termination: (Date/Time)

Outfall 101 12/11/07 1200 ET 12/11/07 1009 ET UV Treated Outfall 101 12/11/07 1145 ET

8. Test Temperature: Outfall 101: Mean = 24.6°C Mean = 24.8°C (24.1 - 25.00 C) (24.5 - 25. 10 C)

Test Temperature: UV-Treated Outfall 101: Mean = 24.6°C (24.1 - 25.1 °C)

9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).

Page 4 of 101

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted December 4-11, 2007 using effluent from Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 2 3 [4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 98 98 98 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 98 Mean Dry Weight (mg)

Test Soluetis (replicate number)

(%Effluent) 1 2 3 4 Mean Control 0.804 0.535 0.540 0.671 0.638 11.3% 0.718 0.745 0.740 0.722 0.731 22.6% 0.709 0.651 0.632 0.737 0.682 45.2% 0.673 0.596 0.556 0.652 0.619 72.6% .0.657 0.628 0.627 0.643, 0.639 100.0% -0.582 0.557 0.658 0.624 0.605 Intake 0.626 0.699 0.522 0.690 0.634 IC 25 .Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 45.2%

Permit Limit: 2.2 TUc

95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC5 0: TUc = 100/IC 25 Page 5 of 101

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Resul6 of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted December 4-11,2007 using effluent from Outfall 101.

Percent Surviving Test (time interval used - days)

Solutions 2 1

(% Effluent) 4 5 6 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 i00 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

Tet Soluetis Data (replicate number)

(%Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 31 32 35 33 27 34 31 34 32 30 31.9 11.3% 33 37 33 34 37 30 34 33 31 33 33.5 22.6% 35 31 35 37 37 36 32 33 36 36 34.8 45.2% 34 38 35 39 35 35 36 40 36 33 36.1 72.6% 38 36 40 35 36 39 35 38 38 36 37.1 100.0% 38 36 41 38 38 41 36 38 37 41 38.4 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 45.2%

Permit Limit: 2.2 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC 50 : TUc = 100/IC 2 5 Page.6 of 101

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.

(Genus species) . (Type / Duration)

Conducted December 4-11, 2007 using water from Intake Percent Surviving Test (time interval used - days)

Solutions 22

(% Effluent) " 3 4 5 I3 67 Control 100 100 100 100- 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

(t Eflueont) Data (replicate number)

(%Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 29 30 31 29 33 36 31 31 31 31.1 Intake 39 33 37 37 33 36 33 38 37 35 35.8 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:

Upper Limit: NA

.Lower Limit: NA

_*TUa = 100/LC5 o: TUc =100/IC 25

(

Page 7 of 101

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted December 4-11, 2007 using effluent from UV Treated Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 2 3 4 - 5 6 I 7 Control 100 100 100 100 100 98 98 11.3% 100 100 100 100 100 98 98 22.6% :100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 . 100 72.6% 100 100 100 .100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg)

Test Solutions (replicate number)

(% Effluent) 1 2 3 4 Mean Control 0.682 0.556 0.830 0.808 0.719 11.3% 0.609 0.664 0.687 0.724 0.671 22.6% 0.801 0.661 0.730 0.767 0.740 45.2% 0.716 0.659 0.599 0.766 0.685 72.6% 0.692 0.688 0.789 0.643 0.703 100.0% 0.650 0.603 0.702 0.643 0.650 Intake 0.691 0.711 0.736 0.698 0.709 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC50 : TUc = 100/ IC2,5 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species I Date . Time Duration Toxicant Results (IC 25)

Pimephalespromelas December 4-11, 2007 1307 7-days KCI 0.52 g/L Ceriodaphniadubia December 4-11, 2007 1035 7-days NaCI 1.07 g/L Page 8 of 101

Water Chemistry Mean Values and Ranges for Pinmephalespromelas and Ceriodaphniadubia Tests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101, December 4- I1, 2007.

Test Sample 11) Temperature ('C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual Initial - Final Initial Final Initial Final (ýLmhos/cm) (mg/L CaCO3 ) (mg/L CaCO 3) Chlorine (mg/L)

Contiol 24.7 24.5 . 7.6 7.5 7.91 7.75 312 57 93 24.6 - 24.8 24.3 - 24.7 7.4 - 7.7 6.8 - 8.0 7.38 - 8.15 7.56 - 8.05 296 - 337 57 -. 57 92 - 94 11.3% 24.7 24.4 7.8 7.5 .7.85 7.74 303 24.6 - 24.8 24.2 - 24.5 7.4 - 8.2 6.9 - 7.9 7.57 - 8.09 7.53 - 8.01 287 - 318 2.%

22.6% 24.7 24.5 7.8 7.5 7.87 7.75 297 24.6 - 24.9 24.4 - 24.6 7.6 - 8.0 6.9 - 7.9 7.56 - 8.09 7.53 - 8.06 280 - 320 24.8 24.5 7.9 7.5 7.87 7.77 277 45.2%

45.2% 24.7 - 24.9 24.1 - 24.6 7.6 - 8.1 6.9 - 7.8 7.56 - 8.09 7.52 - 8.09 255 - 307 7Z 72.6% 24.8 24.5 8.0 7.5 7.88 7.80 254

._ _ 24.7 - 25.0 24.3. - 24.6 7.6 - 8.2 6.9 - 7.8 7.54 - 8.13 7.53 - 8.10 241 - 268 .- -

100.00 24.9

24.5 8.0 7.5 7.89 7.83 234 72 87 <0.10 24.7 - 25.0 24.3 - 24.7 7.7 - 8.2 6.9 - 7.9 7.51 - 8.14 7.56 - 8.13 220 - 250 68. - 76 78 - 98 <0.10 - <0.10 Intake 24.9 24.5 8.0 7.5 7.87 7.82 233 73 89 < 0.10 24.7 - 25.0 24.4 - 24.7 7.7 - 8.4 6.9 - 7.9 7.48 - 8.13 7.58 - 8.09 220 - 251 70 - 74 84 - 94 <0.10 -. <0.10 Control 24.7 24.7 7.6 7.8 7.91 7.91 312 57 93 -

24.6 - 24.8 24.5 - 24.9 7.4 - 7.7 7.5 - 8.1 7.38 - 8.15 7.58 - 8.07 296 - 337 57 - 57 92 - 94 - -

1.% 24.7 24.8 7.8 7.8 7.85 7.92 303---

24.7 - 24.8 24.5 - 24.9 7.4 . 8.2 7.5 7 8.1 7.57 - 8.09 7.57 - 8.08 287 - 318 -

2 2.6 IV 24.7 24.7 7.8 7.8 7.87 7.92 297 24.7 - 24.8 24.5 - 24.9 7.6 - 8.0 7.5 - 8.2 7.56 - 8.09 7.57 - 8.08 280 - 320 4: 4.%

45.2%IV 24.8 24.7 7.9 7.9 7.87 7.94 277 24.7 - 24.9 24.5 - 25.0 7.6 - 8.1 7.6 - 8.2 7.56 - 8.09 7.56 - 8.11 255 - 307

.2 72.6% 24.8 24.8 8.0 7.9 7.88 7.97 254 24.7 - 25.1 24.5 - 25.0 7.6 - 8.2 7.6 - 8.2 7.54 - 8.13 7.57 - 8.16 241 - 268 -

24.9 24.8 8.0 7.9 7.89 7.99 234 72 87 < 0.10 24.7 - 25.1 24.6 - 25.0 7.7 - 8.2 7.7 - 8.1 7.51 - 8.14 7.57 - 8.19 220 - 250 68 - 76 78 - 98 <0.10 - <0.10 Intake 24.9 24.8 8.0 7.9 7.87 8.01 233 73 89 <0.10 24.7 - 25.1 24.7 - 25.0 7.7 - 8.4 7.6 - 8.1 7.48 - 8.13 7.62 - 821 220 - 251 70 - 74 84 - 94 <0.10 - <0.10 Overall temperature CC) Average Minimum Maximum Pimephalespromelas 24.6 24.1 25.0 Ceriodaphniadubia 24.8 24.5 25.1 Page 9. of 101

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for the Pimephalespromelas Test, UV-treated Sequoyah Nuclear Plant Effluent (SQN),

Outfall 101, December 4-11, 2007.

Test Sample ID Temperature CC) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Initial Final Initial Final Initial Final (gmhos/cm) (mg/L CaCO 3) (mg/L CaCO 3)

Control 24.7 24.6 7.8 7.6 7.86 7.75 313 57 89 24.6 - 24.8 24.3 - 24.7 7.6 - 8.0 7.1 - 7.9 7.61 - 8.08 7.55 - 7.98 294 - 333 57 - 58 88 - 92 11.3% 24.8 24.4 7.8 7.6 7.87 7.73 309 24.7 - 24.9 24.2 - 24.6 7.7 - 7.9 7.0 - 7,9 761 - 8.08 7.53 - 7.97 288 - 329 - - - -

21.6% 24.9 24.4 7.8 7.4 7.86 7.37.76-80 27 295

-31 24.7 - 25.0 24.1 - 24.8 7.7 - 7.9 6.8 - 7.9 7.60 - 8.10 7.53 - 8.00 277 311

.*45.2% 24.9 24.4 7.8 7.5 7.87 7.77 281 4 . 24.7 - 25.0 24.2 - 24.8 7.6 - 7.9 6.6 - 8.0 7.55 - 8.12 7.53 " 8.03 259 - 305 -

726%

72.6% 24.9 24.4 7.9 7.5 7.88 7.82 256 -

24.7 - 25.1 24.1 - 24.8 7.8 - 8.1 6.6 - 8.0 7.55 - 8.15 7.55 - 8.05 236 - 278 --

100.0/ 25.0 24.4 8.0 7.5 . 7.89 7.83 235 69 90 24.8 - 25.1 24.1 - 24.6 7.8 - 8.2 6.8 - 8.0 7.52 -. 8.18 7.57 - 8.09 219 249 66 - 72 86 - 94 24.9 24.4 8.0 7.6 7.89 7.83 234 66 89

_____ Intake

_____24.8 - 25.0 24.2 - 24.6 7.7 -8.3 6.9 -8.2 7.52 - 8.16 7.59 - 8.08 215 -256 60) 70 84 - 94 Overall temperature ('C) Average Minimum Maximum Pimephales promelas 24.6 24.1 25.1 Page 10 of 101

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected December 2-7, 2007, showed no toxic effects to fathead minnows or daphnids:- The resulting IC 25 values, for both species, were >

100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant differences from the control during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.

Page 11 of 101

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas-Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphniadubia Tests were conducted according to EPA-82 1-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephalespromelas None

2. Ceriodaphniadubia None DEVIATIONS IMODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephalespromelas None

2. Ceriodaphniadubia None Page 12 of 101

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants,. Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B.

4. Dissolved oxygen was measured by SM 4500 0 G.

5. The pH was measured by SM 4500 H+ B.

6. Conductance was measured by SM 2510 B.

7; Alkalinity was measured by SM 2320 B.

8. Total hardness was measured by SM 2340 C.

9. Total residual chlorine was measured by ORION Electrode Method 97-70.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCI or NaCI.

2. Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephalespromelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphniadubia.

3. Dilution Water Used: Moderately hard synthetic water.

4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 13 of 101

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to FreshwaterOrganisms, EPA-821-R-02-013 (October 2002).

3. Standard Methods for the Examination of Water and Wastewater, 2 0 th Edition, 1998.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 14of 101

Sequoyah Nuclear Plant Biornonitoring December 4-1.1, 2007-Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser-Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling Page 15 of 101

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1.998 -December 7, 2007 Towerbrom PCL-401 Cuprostat-mg/L mg/L PF TRC Copolymer mg/L Azole 0.005 0.011 0.021 0.019 0.015 0.015 0.015 0.015 0.024 0.012 0.024 0.023 0.024 0.120 0.022 0.024 0.022 0.024 0.025 0.024 0.016 0.023 Page 16 of 101

Table 13-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbio logically Induced Bacteria and Mollusks,

~During Toxicity Test Sampling, March 12, 1998 - December 7, 2007 Date !::ii::iiiil*i!ii::iiii:iii:Towerbrom ::: 22'::::::::::::: PCL-401 :i: * ;i:: Cuprostat- N4 1OW:

01/31/2000 <0.002 0ý026!ll~i i i i i i 0.009 iiiiiiiii iiiiiiii i 02/0 1/2 0 00 ::::::::::::::::::::::::::::::::::::::::::::::::: 0 .0 11 0 ,0:2:6iii:i

:ii:iiii:i:: 0 .0 28 iiiiii i !*i!l ...................

02/05/2000 iiiiii}i}iiiiiiiiiiiiiiiiiii:iii < 0.002 ~ ~iii!iii~:i 0,027iiii~ 0.009 ........... i:17 7:iii!iiiiiiii~i~~il~iii!l

<0. 057 :::::::::::::::::::::: :: ,: :::::::::::::.0 : 0.019 ...........................

073!iiii2i::i~ii*:iiiiiii

iii~i::iii:iiiiiii{ < 0. 0 6 :::::::::::::::: : : : :::::::::::: 0 . 1 :::::::::::::::::

. :::::::0 ::::::

............0 ...........

1 2/ 0 0 ......... .......... 0. 1 4 0.. 5............ A.......02 0*i!!i** ...... ***!!!!!

...... ~ ~ i!ii:i* :i:*::*# *:

12/14/2000 #* * * *:::::::::::::::::::::::::::::......:.........::::::::

3 33 < 0.0087 i* *((i{!3!{ 2 i.3i{*((((!{

+:- ..... ......-........--- ::):::: i:: !::ii:: ii 1215200~i~i~i~iiiiiiii~i!!**i*{~iiiiiii!ii::iii!ii*i~~i 0 01 0 .[~iiiiiii~* {[ii!.[I 0 . 20 ..

{.... ........ -.. : :.:.:*i:*iiiiii:i:i~i::i 12/16/2000 <iii~iiiiiiiiiiii~iiiiiiiilii:~iiii

<0.0036 i~iiiiii{**iii~ii{i* 0.020 iii~ li i**i~.... .....

082/208/27/2001 <.09

<0.0096 *3;i~N i!i* 0.2 0.021 ii}**N~i{i}i ii~l*i*2:**}ii**** !!i{ 0 8/2 8/200 1 iiiiiiiiiiiiiiiiiiii((((iiiiiiii}i

                                              ...                                                <0 .0 12085 iiiii((~i                 i iiiiii~iii                 0 .02 1                                                                        ~~iii!!!!......i}:iii~~iii!!((

08/29/2001 iiiiiiiiiiiiiiii;iiiiiiiiiiiii)iii! "<0.00!94 [iiiiii~* }ii{i..~ 0.020 --0{~ii* N ii*iiif 00.5i~iii~~ii;i{!{~i

                                      .---....--..-. '...-.-...-. , w*.. -,.

120/100/21 -.... ..--.-.-...........

=*:::::=::=::::::: 0 0 4 =======================

                                                                                                                = = ..
                                                                                                               ....             ==.......
                                                                                                                          ...............  ===========================iiiiiiii{ii
                                                                                                                                         = =.=====
                                                                                                                                              .... .. -=======
                                                                                                                                                     ......  -                         E m
                                                                                                                                                                                                  ~iilim i{~iii!iiii a::)::*:!;::i::):

iiiiiiiii 122/2

                     /1 00          !3iiiiii!*iii{*{*{}i
                                         .......                                                   0 . 12 ~i~::i((

... :..... :..0......{ii* .......... * {i~i((ii i!!i~i(({*ii*!~i!ii!!ii 11/26/2001 21 0 l .. .....:iiiiii=:~i . 13 1/2 ....

                                                                                                 <0 0 1819 ::::::::::::: : :::::::::::::::
                                                                                                 <0.0 i~*!!    j* { iiiiiii~!!                ..... . 2 0.02. 2                 ii ii~
                                                                                                                                                                                        *333333**33:****333333-ii~:i::;:i::{::i:}:::!ii 0.05iiiii)iiiii!)il

..... ... iii~iiiiiii ii~i}~iii Page 17 of 101

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 7, 2007 Date Towerbrom..,..,,.. PCL-401 CL-33 Cuprostat- H-43 M: Mhlne mg/L mg/i..1 mg/L' Mgf-L PP gF mgLTRC Phosphate Copolymer DTMAD mg/L Qa Azole 01/02/2002 < 0.0079 0,G23 0.02 0J)06 01/03/2002 < 0.0042 ..................................... 0,.023- 0.014 ....

....... i~ii~ii:ii 01/04/2002 0.0124 0,Q2 4 0.014 -- 0,009

                                                                                                                       ........................................                                "............... ......

i:?i:i:i:ii!{ ii#i:

01/06/2002 < 0.0092 , ........... 3 0.018 05/09/2002 < 0 0 42 ::::::::::i::i::::

                                                                                                     -                       f4.057i}:*i{*i!{*i~!{i**i:i*iii*}i}*

:::ii:::i:i::: 0.00014.iii{:*i::*::*:{:i~~~i:i ~i!:ii~!*iiiiiii:l 02/21/2002 <0.0042 ........ 0.018 01/13/2003 <.00004:04000:

                                                                                                <0.006                     .               5019 01/125/2003 iiiiiiiiiiiii!ii* i:i{iiiiiiiiiii 08/05/2002                                                                                                     PO5$.. . . ...

0.0058 *~~iiiiiiiiiii~*{~***l~ii~ii

                                                                                           <<0.0043                                                                      0.018.iii(({iiiii{::::::::::::i::::::::::::::::::

0.020

                                                                                                                                                                                                    .0.009 08/07/2002                                                                   < 0.01072 . . .05: ":0                                                       0.1 04/06/2002 08/08/2002A                                                                  <00189
                                                                                           <0.00691:                                                                     0.02                               0 14.-ý-ý
                                                                                                                                                                            .1               t5 04/08/2002                                                                    < 0.001                                                .                      0.021 102/06/2002 0 5 / 5 / 0 0                                                    < 0.0049-                * *; ii#i                          #*..............                                          .............................                          .................

10/07/2002 0.0153 ............. 008 00 102/08/2002 0 26 /2 0 80 /0 :::::::::::::::::::::::::::::: <0.0042 .. .. ~~5

                                                                                                                         .. . .. .. . .. .. .                           0.018230               ..:....    :...........         -?!!}*!!iS5:5!

ii:::i)::::ii:

8/0

                  /04/17/2003
                        /2 0 02 0102/09/2002                              ::ii::~iii:::i:i:::*::              <<0.007 0.01243    8 ::*iýiý'    ii}ý:!:*iiiii........

0 .0 107i::ii1:iii:i{:~i*;Q3

                                                                                                                                                                             . 19 0.018200              ...
                                                                                                                                                                                               *:!**ii:o:*:**
                                                                                                                                                                                                        ..........:*:**::i:*:*:*:

101/020.0013 5 --

                                                                                                                                    '023            --                   0-1                         00 01/2/003<0003                                                                    ..           ...            ....

01/014/2003 i <0.01 0,0.02

                                                                                                                 ...............................................................                                  .. ........                               ......::i~i:**:*::*iii::i{:

108/06/2002 <0.0 1047 ~.i~iiii~

                                                                                                                                        ~~i:iiii:iii
                                                                                                                                      .....                                                      iii~iiiiii                                                  ::~i:i::i:i:i.i:i:::{:*i:i 1 0 /0 7 / 0 0 2.00                                                 15 3              -                  .'.-.','..-.----.-'..ý-...
                                                                                                                                   .*........                                       8:::::::

0:::::::::::0::1 ::::::::::9:::::::: 108/0 8/2 00 2 ..................................... <0 .0 0 6 219 :::::::::0:::::: :0 ............ .................... 1:::::::::::::::X.0 9 ::::::::::::::::::::: 04/06/20032007 10/10 /2002 :;ii{i!ii**iii:ii*ii!i}iiiiiii!iii:i.*ii*i <0.0134 0 !* -4ii~lliii{ iiii~i~i 0.018 J0,ii~ l~ i{*!!~ !iiii~~i{*iiiii{~ 04/09/20032003 0.021 .1 04/10/2003 { 010/12/20023 <<013 .0 4 ........ ((i(((({* .....0.0018

                                                                                                                                    * (({i(({:{                             .0219             .0.1    ***(({~iii~i~*{:i*!{*((i((((!;((ii((i{i 01101 0/13/2003 iiii:iii :i:! <0                                                  <0.006 06          i~:i:i:iii:i          2:iiiiiii~i                      0.019. 19:::::::::::::::::::::::::::::::

A0.:i

                                                                                     . .. .                               :::::::::::::::::::::::::                                               x.......  ......      .....

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i:ii:.............1i??}i!;::

04 1 /01/15/2003 <-ý0.0063

                                                                                                   . 1 3 iiiiiiiiiiii~!i:iiiiiii{i!iii.{

0 00.020i~iiii~iiilliil 0 1 iiiiiii i !i~ii !i!ii~iiiii*iiiiii~l:i

                                                `*/*/2
                                                     *iiii~ii**:!!i:!::*:**i 3**iii**:*;;::i**:::i.::*             < .0 7                    .. . ...... ....... 0 0 2..,,,,,,,,, ...

~iiiiii~i{!i~~!!i~!{!((ii{iii*ii:ii:... ........ ..... ...

                                                                                                                                                                                                               ..,,,.!;.i-:ii~i:{i:i{iiiii~ii::        ~::{::ii~i::i~:

Page 18 of 101

Table B-I (continued). S equoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 7, 2007 Date Sodium Towerbrom PCL-1222 PCL-401 CL463*36'1ý Cuprostat- H-10*116A4 Hyluichlo*ite mg/L rng4. mg/L fng/I.,L. PF mgtL mfi:L TRC Phosphate .. . .... . -... ......... Copolymer ::-DMN' l AD mg/L Qa*a: IRO Azole 06/15/2003 <<0.0045 .. .. . . .... - ... .. . ...... ......... ::::: :::::::: 06/16/2003 - <0.0037 0,057 0.020 - - R022 06/17/2003 < 0.041 0.014 :0.0048 - ,4

                                                                                                                   .........................................il~iiii{::: :i~ i!!((i~ii:i~..:.. .............

06/20/2003 05/06/2004. <0.0048 0.t26 .0 .014 0.014 0 . ..... 0000 - 0.025 08/03/2003 <0.0050 0........**..0 0.02... ....... 0 ..... / 4...... 2 47 ........... 0 08/05/2003 <0.0085 :::1 . 0.020 ... . 00 08/07/2003 0.0152 0.4157 0.020 19: 24 0/07 /2003 0.0223 . 0*057 0.020 . 0009 02/05/2004 10/09/2004 <<0.001 0.0.00348. 3 . 0141 0.1 0.0200092 0009 05/04/2004 < 0.0128502 0.02900.2 10/108/2003 0 0 83

                                                                              <0.0106                x 0 :1 :5.7;ii}*}iiiiiiiiiiii!{!~*3

::::::::::::::::: 0.020 :::::::::: : ::::::}i:i:i{:i:::::iiiiiiiiii 0201204:::::::::::::::::::::::::::::

. 0 9 ~ i~iiiii i iiiiiii*i~i 0 0 9 **:::*3*:::***:::**:::***:.............

0210 00/09/2003 <0.0181.0 4 iii~iiiiii~* *~iiis!!ll .0 9 ili~~iiiii~iiiii * - 0.022i!!i!!!*i**i*~i :ii~iiiiiiiil~iiiiiii 05/09/2004 10/10/2 003 ::*::**:*:*:iii~*:i :**~*:i::*:iii*::*::*:*

<0.01504 0 0 330.0~ ii::::i:::!*

:::::::::::::::::: 0.&

0 .01

                                                                                                              *:::2:::::::iii~ii::i:
                                                                                                                             ......           0.020 24**~~iiiii~i*i1i_::::::::::::::::::::::::::::             :: ::**:i: :::::::i**::

02/4/00)i~iiiiiiiiii**i~i~ii!i**ii~iiiiii~~iii~iii!! 0 01 4 iiiiiiiii !* * .......... i*{iii~i 0 .0 9 ::::::::::::: ::::::::::.... iiiiiiiii~ii 07/06/2004 <0.0077 0.0200,2 02/02/2004 ii!iii*ii*ii:iiiiiiiiiiiiiiiii{iii <0.0034 0,020i*!ii!

                                                                                                             !* iiiiiii~i                     0.0140:9::::: : :::::::                                          *~iiii              {iii 0 2 /0 4 /2 00 4 *iiiiiiiii5iiiiiii* {iiiiii*iii}{lii            0 0 140       :iiiiiiii 0 i.012i:}:iiii:l                           09                  ...............

ii:i:i::::i:i::: ii~iiii:::i~ ii~iiiiii:* 02/0 6/2 004 Fii!!*!*!ii~*!iiiiiii~~~iiiiiiii*iiiiiii 0.0 1052 ...... c i.ii ii!iiiiii~ 0 .009 iiiiiiiii::3*3# !*::}iil i a t::::i

0*:*:i:*:i:i:ii!i~ii::iiii:i~

                                 /0/204             iiiii:ii:{i:ii:{ii*i:::i*  0 0 2 3 ~ ii((iii((i          i~ .. iiiiiii~!{li .0 9 ii}!ii   i~ iii(({::::::::.:..::::::.::::..::.: :::::..:...::::::::::...::

07/ 9/00 **ii*.*:**i:.i~i::):3:)i:::**ii3*)*::*)3:}**:3i:: 0 01 2 *:iiiiii~*::*~ i i~ i*iiii~ii~ii .0 0 :iiii::::!*i..:........-::...:*: =========== * >3*

                                                     *::*-::*-:::i*::i*:
                                                      *:::**::**:::3*::* ::*i~:<            *3:;*:;#      -*.#*33133-;3;33333                                #33335     ## ;33#  **!3                         :::::::::::::.:..:.::.::::::::::..:::.

Page 19 of 101

Table 13-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 7, 2007 Date sodmm-*..:* :*!ii Towerbrom P(M::i *12'i! PCL-401 CE*-(A***'!:- Cuprostat-PF R :101ii Nalco H ti~i Hyocllrtý mg/L rnviiiii~ i~il- iii mg/L t i;i* ii~i:i mg!L iii* !;) 73551 mj*~ii 11/0 7/2 004 iiiii*!ii~**~~*ii!{ii*iiii~iiiiii~i

                                                                  .... < 0 .0 187                                       :: ::::::::::::: 0 .0 104iii!**:!i*!~ i~

0 ,M)::::::::::: ::::::::::::::::::::.........

                                                  .............................                                    ......... . . . .....                               ....:i~iii:*:i:!i:i:*#

11/12/2 0 04 :}ii~~~~~* ii:ii;::i::i::::i: < . 2 3 il:iiii:i i ;i~:}::}::

                                                                                                                                      ......          0 .0 176          ::::::::::::::::::::::::::::::::::::: :                               ::::::i:iiii~:i::

02/09/2005 .....}::::::::::::::::::::::::::::::::: <0.0 042 :i:ii:ii:~ 028 :ii:::::i:: 0.0 10 :*:!*::*~i:*::::*: ........ 02/07/2005 i!iiiiiii~i!i~!iiiiiiiiii <0.004 116 Q Q2S*ii~ii}i~i ... 0.010 0.007~!:*i:ii!i*i*!ii:i~ii~ii*i!

i~iii**ii*

0211 ii~iii}**:iii!iiiii**i!ii~iiiiii 205 0. 15 **}}*iii~ *) *!iiiiiiii 0 01 **}i~i~~ii~i~i~*! " iii!:*i~...........0.0.*li!i!!i~il 06i:ii;:ii::i:i::ii:i

                                  .-0/ 0 0 i:iii::::i:i::i:ii 0..../.08..../....--,.-....                                    0 0 63                                      "'.....................0.....................

- i::**:;:i*

                                                                                                         ......             ::*::i i::ii 0.,...0..:080::::::::::::::::::......::::::::::::::::::::::::::::                         ...........

02 0/2 0 0 .0::::::::::::::::::9::::::: 0 .0 10********i:::::::::::::

                                                                                                                                                                      *i*i~ ii~ ***ii~                                 iiiii??ii~**! ii-                                     *i~i~ii~~

060}}:ii:i:i:}~~iiii:i 6205 i:::iii::iii~i:i: .0 4 3 i:::*:i:*:*::::*:*:*:*.:.4:::*:**}:*~~i)* X...... ::::::::::::::: ::::::::::::::::::::::}::::::::X :::j::: :iiiiif 02/10/2005::::::::::::::

                                                                          <0.0042
                                                      .....................................               ::::::::::::::::::::::::::::::::::        0.010:::
                                                                                                                                                                              ;*~  ~~iii~  ii~~#  ...i                i!#i! i!iiiiiiiii ..............

02/ / 0511/2005 . 2 5 0~iii*!iiii!**!i! i!:::!::ii::::iiii':i:)i:ii!!::~:i:}~~~lii!ii*;}i~~~i~!i iiliiiii}i}ii!!!~}.0155........10 0.007ii ::::::::::: 0 6 0/2 0 .0....0....0....6 .. . 3.. . ............... ......... ===================== ::::::::::::::::::::.:::::,:: 06/206/2005 :***i})*i**~~}i{ii 0.020943 "

                                                                                                          *i}iiN }}i** .o
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Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 7 December 7, 2007 Date Sodium Towerbrorr PCL-401 CL-3: Cuprostat-PF Nalco 4Hyp ohiote mg/L mg/L mg/ilt' mg/L 73551

                                 -g/L                    TRC         Copolymer   D)MAD       Azole      mg/L EO/PO 11/12/2006                                              0.0055 11/13/2006                                             0.0068 11/14/2006                                              0.0143 11/15/2006                                              0.0068 11/16/2006                                              0.0267 11/17/2006 ...........                                  0.0222 11/26/2006                                              0.0188 11/27/2006                                              0.0138 11/28/2006                                              0.0120 11/29/2006                                             0.0288 11/30/2006                                              0.0376 12/01/2006     ..                                      0.0187
 *05/28/07 05/29/07 05/30/07                                              0.0084                                          0.017 05/31/07                                              0.0103 06/01/07                                              0.0164                                          0.017 06/02/07   ......                                     0.0305 12/02/07                                              0.0241 12/03/07                                              0.0128 12/04/07    .......       ..............              0.0238 12/05/07             ..........
              .............. ......* -.......... .... 0.0158 12/06/07                    ..                .       0.0162 12/07/07                                              0.0175 Page 21 of 101

Sequoyah Nuclear Plant Biomonitoring December 4-11, 2007 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 22 of 101

BIOMONITORING CHAIN OF CUSTODY RECORD Page I of 1 W I 'Client: TVA Environmental Testing Solution, Inc. I Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Container Flow ,-7 Sample Description Number & MGD Rain Event? Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-

                                                                                                     "--             Inches                        Number         Temp.                          ance

(°C) SQN-101-TOX Comp 12/02/07-12/03/07 0738- 2 (2.5gal) NA X X - . -$ ____________ 1__ 0638 ____ ____ ___ i . I' SQN-INT-TOX Comp 12/02/07-12/03/07 0807- 1 (2.5 gal) NA X X z.,4 LnlzoI 0707 OH __Sample Custody - Fill In From Top Down , 4 A., -. D JA Relinquished By (Signature): Date/Time Received By (Signature): . Date/Time Chevy Williams / 12/03/07 Sonic Delivery 12/03/07 Sonic Delivery 12/03/07 ETS ,12/03/07 __A 5 q HM Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 35 1 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Container Flow *I'* Qot.3t55i Sample Description Number & MGD Rain Event? Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-Inches Number Temp. ance (°C) SQN-101-TOX Comp 12/04/07-12/05/07 .0752- 2 (2.5gal) NA X " (\ X'l2c. '-s _________ ______ _________ 0652 2z `!1 4) SQN-INT-TOX Comp 12/04/07-12/05/07 0824- 1 (2.5 gal) NA X 0"412,32. 32S 0724 .3 Z.,- _ Sample Custody - Fill InFrom Top Down 4- C.uai - Relinquished By (Signature): Date/Time Received By (signature): a 1/2m'A Chevy Willi ams 12/05/07 -7 Sonic Delivery 12/05/07 Soi Delivery 1/0 12/5. Sonic. Delivery . 12/05/07 ETS .12/05/07 d ______________________________________ & Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not anive until the following Monday.

L BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 lient: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 35 1 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Container Flow Sample Description Number & MGD Rain Event? - Volume (Mark as Appropriate) Laboratory Use Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-Inches Number Temp. ance (°C) SQN-101-TOX Comp, 12/06/07-12/07/07 .0750- 2 (2.5gal) NA X . ________ ____________ 0650 H,/o (3 SQN-INT-TOX Comp 12/06/07-12/07/07 0806- 1 (2.5 gal) NA X 0.6 . 0706 -3 - - Sample Custody - Fill In From Top Down 4 Cu. 4- . Relinquished By (Signature): Date/Time Received By (Signature): *U Date/Time 4__ Chevy Williams 12/07/07 / S Sonic Delivery120 Sonc/~` c-iv--/ So12/07/7iverT 12/07/07 Sonic .D Ielivery/ 12/0 .7/07 ['-- ETS,, _ 12/07/07 1!.SC Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Environmental Testing Solutions, Inc. Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: TVA Co'unty: Hamilton Facility: Sequoyah Nuclear Plant Treatment: Non-treated NPDES #: TN 0026450. Outfall: 101 Project #: 3bS!\ I Dilution pn Dilution prep (%) Test organism informationi: Test information: Organism age: 7-.0..1s Io A %3 OLb Randomizing template: q'-.IoI Date and times organisms k-O"*-al I 4oO0 Incubator number: were born between: zc_ Organism source: fýT'A &a-cr\ 9e --ff*- cF Artemia lot number: 0 Transfer bowl information: pH = Temperature C Total drying time: I+OmoAS "3-I "L "L~.. Date / Time in: I-L\,-11. I Average transfer volume:. Date / Time out: .'-I " ,..*L Oven temperature: Io" C... Dailyfeeding and renewal information: Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 -1 vqS t1250 i-L- o\-' 4,, nI' pj'&.o0", ff-2 U4 1.414S its_

                                                                      \Z-ot-0 Z       _                   -1O           O 5. -U +23 0-12i 3      '2.-(fl--i        61                                IIt-Il___                     01
                                                                                                -O011         61a0s7.,z7 4"-7                    _

4 lz-ot-ui Oac3c IqO0 .OO oL -01 011?01. i k2'. "t 6 17-o1- G fsi o IF\ IL-oC-+1 alLa1l. a %L5 7£ Control information: Acceptance criteria Summary of test endpoints:

             % Mortality:                                    67.                   -20%                 7-day. LC50            )107.

Average weight per initial larvae: 0.6.39 ..*W"K: ... NOEC tocs7 Average weight per surviving larvae: 0.65 3 & - 0.25 mg/larvae LOEC > 166 7. C ChV >ioo, IC j > /007. Page 26 of 101

Inc. Page 2 of 6 Environmental Testing Solutions, Species: Pimephalespromelas Date: .L-C)-A- c\, Client: TVA / Sequoyah Nuclear Plant - Non-treated Survival and Growth Data Day CONTROL 11.3% 22.6% A B C D E I F G H I J K[ L I 0 /0 ' 1 C0 /0 10 t0 /0 /10 Q 10 /C) 1C I /0 c /- / I0 Ib/ o /0 /0 /( 'o t 3'/ 0(c) (0/ 0C)/ 0 0 /0(0 /01/0 5 -tO to to to t10 0 to 1 0 /0 '0 10 to 6 C0 /0 /0 1 0* '0 /0 /0 '0 1O 7 [0 1t /0 t! 10

                                                                                        /0                         10   /0      /0        /0         tO A     Pan weight (mg)

Color identification:'At.I.oY4 RA 0AR1-t S' 361I.I.9.S1 35t 11 CSiI-( Analyst: Lj-B = Pan + Larvae weight (rag) /. , tZG , -Z&.L l L Z --. 1 ,' o,7.tZýj' *z S 26, U$ Z O.W37,1 Z ,4. Analyst: IM) Larvae weight (mg) = A- B (4 1?.o'4 -;**s.% S-4 o ,.11 11%'.4S 1-4*0 1,21 ,ZI-O 7 (. l .*. S,-\ -1.31 I Weight per initial number " of larvae (mg) / P,\ '\*- A? , C / Initial number of larvae c e O0* O" 0" t ' t* t) b" O'*

0" Average Percent weight per reduction j7.

initial from control .0..O2 number of (%) larvae (mg_) Comment codes: c = clear, d dead, fg fungus, k = killed, m = missing, sk sick, sm unusually small, lg = unusually large, d&r decanted and returned, w wounded. d r

                                                                     "                                                       Calculations and data reviewed:

Comments: Page 27 of 10f

Page 3 of 6 Environmental Testing Solutions, Inc. Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: \--o-C7-Survival and Growth Data Day 45.2% 72.6% 100% 1 [O PR SSU T V XV x 0- o D b /0 1C).'0 /1 /0 /0 '0 /0 /0 S1 / I0I0 (0 /0 /0 (0 (c0 /C C0 /O /1 2 1O ,ID / 0 / 0 /0 /0 /0 /0 /0 /0 3 /C IU t to ' (0 /0 (0 /0 /0 /0 to '0

                                            /0         o      /0     /0/o..                         /0               /0     /C0                   0 1F3             10 10               /C) 'ý0 10      Q)       0       1c                      I~C)     10 6                 1       /!ou0 1r3(II0                  C1 C) '1               /tIu                                     /0 7                                    %5 iz 1c           j-0    to     (0      I0         0         10      10          /0      10 A Pan weight (mg)

Color identification:At* W t... R** *.O t.t qq *,l **.* t. q3 (0.4 5(0.% t3. Analyst: B = Pan + Larvae weight (mg) Analyst: MM " z... 1S.t.., t9.%iO Z,.ii jq.o. zo. r", , "1 ..6.2. Larvae weight (mg) A- B Weight per initial numbero'o ', ,$V Ž"' . " of larvae (mg) C / Initial number of larvae \0 ". " " ' " Average Percent weight per reduction initial from control -o. rsLoO5 number of (%) larvae (mg) Comment codes: c = clear, d dead, fg fungus, k = killed, m= missing, sk sick, sm = unusually small, Ic unusually large, d&r = decanted and returned, w = wounded.

                                                                                                        --     Calculations and data reviewed:

Comments: Page 28 of 101

Page 4 of 6 Environmental Testing Solutions, Inc. Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: I )- - 0 1- Surrvival and Growth Data Day 100% Intake Y Z A BB ID oj'cb Ito 0 C 10 Q10 /J 2 10 /0/0 /o 3 1 /O / 1c) 4

                                                                          /0     IQ     /0D        Q C) 10      10        /

6

                                                                          /G3    /0      '0        /0 7                                  .k (0 A = Pan weight (mg)

Color LtbtW lokt..k identification: 1 1{l3q.4 1i. DO Analyst: &;G B = Pan + Larvae weight Analyst: ,)/ Larvae weight (mg) A- B Weight per initial number of larvae (mg) C / Initial number of larvae " "' .0 Average Percent weight per reduction initial from control number of (%) larvae (mg_) Comment codes: c = clear, d = dead, fg = fungus, k killed, m missing, sk sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w.= wounded. Calculations and data reviewed: Comments: Page 29 of 101

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11, 2007 0 0

    -5 0                                                                                 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)

Species: Pimephalesprornelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 3551 Reveiwed by: Not for Cornpliarice Assessment, Internal Laboratory QC Co,,entration(%) Rtph-cet, ll05aenmb~rof FInsnalarettbroca, A-P.n-eight(mg) B-Pa..-L-s'.. L[-. -ewght(ng) Wighet/Sorv-A0nc M--1wdght/SevAinZg C-erertnt e*riaton W.Izht/Irsst sn.stsrs Mec-s.rA-iw Meanwtlhhl/Wthi1 Co~lltM, %I rof P-MrtC ,dustdolfrus

                                                                                           - helttl (lm)        -A-B          numbre lar e. (nia)  nsunmb,roltavc (se   5'tL.'5 p'rlOassO       Loeo (ne,)             (%)            ber
                                                                                                                                                                                                                               , ,au            e     on           I        ronteet (%f A             10                 t0               12.87            20.91             8.04               0.804                                                          0.804 Control           13            10                 10               14.89            20.24             5.35               0.5350638                                  20+0                0.535                              0,638                 20.0            Not Applicable C             10                 10               14.88            20.28             5.40               0.540                0.0.540                                                      100.0 D             10                 10               15.96            2267              6.71               0.671                                                          0.671 E             10                 10               13.81            20.99             7.18               0.718                                                          0.718 113               F             10                 10               12.82            20.27             7.45               0.745                0,731                  1.8                0.745              100.0           0.731                   1.8                 -14.7 G             10                 10               15.09            22.49             7.40               0.740                                                          0.740 H              10                 10               15.13            22.35             7.22               0.722                                                          0.722 1             10                 10               13.56             20.65            7.09               0.709                                                          0.709 22.6%             J             10                 10               13.77             20.28            6.51               0.651                0.703                  12-0               0.651               97.5            0.682                 7.2                   -7.0 K             10                 10                14.51            20.83            6.32               0.632                                                          0.632 L             10                  9                15.06            22.43            7.37               0.819                                                          0.737 M              10                 10                14.28            21.01            6.73               0.673                                                          0.673 4,            10                 10               14.39             20.35            5.96               0.596                0.619                   8.6               0.596 0             10                 10                12.70            18.26             5.56              0.556                                                          0.556 P             10                  10               13.34            19.86            6.52               0.652                                                          0.652 10                 10                14.42            20.99             6.57              0.657                                                          0.657 72.6%             R             10                  10               12.74            19.02             6.28              0.628                0.639                   2.2               0.628              100:             0.639                 22                     -0.2 S             10                  10               14.08            20.35             6.27              0.627                                                          0.627 T             10                  10               14.22            20.65             6.43              0.643                                                          0.643 U             10                  10               1435             20.17             5.82              0.582                                                          0.582 V             10                  10               14.64            20.21             5.57               0.557               0.605                   7.4               0.557 1               100 1o                  10               14.56            21.14             6.58              0.05 0.658                0.605_____              7.45              0.658              100.0            0.60S                 7.4                     5.1 X             10                  10               13.98            20.22             624               0.624                                                          0.624 Y             10                  10               14.93            21.19             6.26               0.626                                                         0.626 100% Intake          Z AA 10 10 10 10 9

o10 13.97 13.85 15.00 19.07 21.90 J 699 5.22 6.90 0.699 0.580 0.690 0649

                                                                                                                                                        .20.96                8.7               0.699 0.522 0.690 97.5            0.634                 12.9                    0.5 Bit Outfall 101:                                                           MSD =           Minurum Significant Difference Dunnett's MSD value:           0.1076                                  PMSD;           Percent Minimum Significant Difference PMSD:                           16.9                                                    PMSD is a re*sure of test precision. The PMSD is the minirorsmpercent difference between the control and treatmenit that can be declared statistically significant in a whole effluent toxicity test.

Intake: Dunnet's MSD value' 0.1472 Lower PMSD bound determined by USEPA (10th percentile) = 12%. PN.S1: 23.1 Upper PMSD bound deterreined by USEPA (90th percentile) 30%. Lower and upper PMSD bounds were determined from the 10th arnd 901h percentile, respectively, of PMSD data flomEPA's WET hsterlaboratory Variability Study (USEPA, 2001a, USEPA, 2001b). USEPA 2001a, 2001b. Final Report. ltterlaboratory Variability Study ofEPA Short-term Chrooic and Acute Whole Effluent Toxicity Test Methods, Voluesses I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005 US Enivironmnental Protection Agency, Cincinnati, Ot

TVA Sequoyah Nuclear Plant, Outfall 101 Non-treated C December 04-11, 2007 Statistical Analyses Larval Fish Growth and Survival Test-7 Day CGrowvth Start Date: 12/4/2007 Test ID: PpPFCR Sample lID: TVA./ Sequoyah Nuclear Plant, Outfall 101 End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.8040 0.5350 0.5400 0.6710 11.3 0.7180 0.7450 0.7400 0.7220 22.6 0.7090 0.6510 0.6320 0.7370 45.2 0.6730 0.5960 0.5560 0.6520 72.6 0.6570 . 0.6280 0.6270 0.6430 100 0.5820 .0.5570 0.6580 0.6240 Transform: Untransformed Rank I -Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 0.6375 1.0000 0.6375 0.5350 0.8040 20.018 4 0.6844 1.0000 11.3 0.7313 1.1471 0.7313 0.7180 0.7450 1.812 4 22.00 10.00 0.6844 1.0000 22.6 0.6823 1.0702 0.6823 0.6320 0.7370 7.188 4 20.00 10.00 0.6823 0.9969 45.2 0.6193 0.9714 0.6193 0.5560 0.6730 8.597 4 19.00 10.00 0.6290 0.9191 72.6 0.6388 1.0020 0.6388 0.6270 0.6570 2.223 4 18.00 .10.00 0.6290 0.9191 100 0:6053 0.9494 0.6053 0.5570 0.6580 7.391 4 18.00 10.00 0.6053 0.8844 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93847877 0.884 0.71715058 2.53040028 Bartlett's Test indicates unequal variances (p = 5.44E-03) 16.5491142 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 100 >100 1 Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 95% CL(Exp) Skew IC05 36.221 IC 10 87.670 IC15 >100 1C20 >100 IC25 >100 IC40 >100 IC50 sqn_ 2-04-07data

                          >100

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated 0 December 04-11, 2007 Statistical Analyses Used for PN4SD calculation only. Larval Fish Growth and Survival Test-7 Day Growth Start Date: 12/4/2007 Test ID: PpPFCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCH-R-EPA-821 -R-02-013 Test Species; PP-Pimephales promelas Comments: Non-treated Cone-% 1 2 3 4 D-Control 0.8040 0.5350 0-5400 0.6710 11.3 0.7180 0.7450 0.7400 0.7220 22.6 0.7090 0.6510 0.6320 0.7370 45.2 0.6730 0.5960 . 0.5560 0.6520 72.6 0.6570 0.6280 0.6270 0.6430 100 0.5820 0.5570 0.6580 0.6240 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.6375 1.0000 0.6375 0.5350 .0.8040 20.018 4 11.3 0.7313 1.1471 0.7313 0.7180 0.7450 1.812 4 -2.101 2.410 0.1076 22.6 0.6823 1.0702 0.6823 0.6320 0.7370 7.188. 4 -1.003 2.410 0.1076

         .45.2        0.6193       0.9714          0,6193      0.5560         0.6730       8.597        4             0.409         2.410     0.1076 72.6        0.6388        1.0020         0.6388      0.6270         0.6570       2.223        4            -0.028         2.410     0.1076 100       0.6053       0.9494          0.6053      0.5570         0.6580       7.391        4             0.723         2.410     0.1076 Auxiliary Tests                                                                                    Statistic                  Critical                   Skew        Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01)                                     0.93847877                    0.884                 0.71715058 2.53040028 Bartlett's Test indicates unequal variances (p = 5.44E-03)                                       16.5491142                 15.0862722 Hypothesis Test (1-tail, 0.05)                NOEC          LOEC           ChV          TU          MSDu         MSDp          MSB          MSE         F-Prob        df Dunnett'sTest                                    100         >100                        1       0.10756027   0.16872199 0.00867097 0.00398382 0.10239999            5, 18 Treatments vs D-Control sqn_12-04-O7data

TVA / Sequoyah Nuclear Plant, Intake arc Non-treated December 04-11, 2007 Statistical Analyses Larval Fish Growth and Survival Test-7 Day CGrowth Start Date: 12/4/2007 Test ID: PpPFCR Sample ID: TVA I Sequoyah Nuclear Plant, Intake End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHl-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: . Non-treated Conc-% 1 2 3 4 D-Control 0.8040 0.5350 0.5400 0.6710 100 0.6260 0.6990 0.5220 0.6900 Transform: Untransformed I-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6375 1.0000 0.6375 0.5350 0.8040 20.018 4 0.6375 1.0000 100 0.6343 0.9949 0.6343 0.5220 0.6990 12.863 4 0.043 1.943 0.1472 0.6343 0.9949 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.91297191 0.749 0.32811175 -0.7982733 F-Test indicates equal variances (p = 0.48) 2.44667292 47.4672279 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.14716277 0.23084356 2.1125E-05 0.01147096 0.96716261 1,6 Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 95% CL(Exp) Skew IC05 >100 IC10 >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 sqn_] 2-04-07data IC50 >100

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11, 2007 0 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) 0 Species: Pimephalespromelas Daily Chemical Analyses Project number: 3551 Reviewed by: _ Concentration Parameter Dav 0 Da I Da 2 Day 3 IIIaIDay 4 Day 5 Ina Day 6 pH (SU) 7.83 7.56 7.381 7.59 7.83 7.75 8.061 7.67 8.10 7.71 8.15 7.921 8.021 8.0-DO (mg/L) 7.7 7.5 7.7 8.0 7.6 7.6 7.7 6.8 7.6 7.27.6 7.9 7.4 Conductivity (jimhos/cm) 296 310 337 304317 Control Alkalinity (mg/L CaCO 3) 57 57 57 Hardness (mg/L CaCO 3) 92 94 92 Temperature (TC) 24.7 24.5 24.6 24.3 24.6 24.6 24.7 24.3 24.6 24.7 24.8 24.3 24.7 24.: pH (SU) 7.58 7.53 7.57 7.57 7.67 8.00 7.67 8.04 7.71 8.09 7.92 8.02 8.01 11.3% DO (mg/L) 8.0 7.5 7.6 7.9 7.9 7.7 7.4 6.9 8.0 7.2 7.8 7.9 8.2 7._ Conductivity (t~mhos/cm) 287 315 312 318 293 296 302 Temperature (CC) 24.7 24.5 24.6 24.5 24.7 24.3 24.7 24.5 24.6 24.5 24.8 24.2 24.7 24.! pH (SU) 7.58 7.53 7.56 7.54 7.69 7.81 8.04 7.67 8.05 7.71 8.09 7.93 8.05 8.0( DO (mg/L) 8.0 7.6 7.6 7.9 7.9 7.6 7.6 6.9 7.9 7.1 7.8 7.8 7.91 7.( 22'6/ Conductivity (itmhos/cm) 280 305 304, 320 285 288 3001 Temperature ('C) 24.8 24.5 24.6 24.6 24.7 24.6 24.7 24.5 24.6 24.5 24.8 24.5 24.9 24. pH (SU) 7.58 7.52 7.56 7.56 7.70 7.86 8.07 7.69 8.05 7.72 8.09 7.96 8.07 8.01 45.2% DO (mg/L) 8.1 7.6 7.6 7.8 8.0 7.6 7.6 6.9 7.9 7.0 8.0 7.8 8.0 7.( Conductivity (ýtmhos/cm) "263 287 281 307 267 255 280 _Temperature ('C) 24.8 24.5 24.7 24.6 24.7 24.4 24.7 24.6 24.7 24.5 24.9 24.1 24.9 24.( PH (SU) 7.54 7.53 7.57 7.58 7.71 . 7.86 8.08 7.72 8.04 7.79 8.13 8.05 8.09 8.1( DO (mg/L) 8.1 7.6 . 7.7 7.8 8.2 7.6 T7.8 6.9 8.0 7.1 8.0 7.8 8.2 7.: Conductivity @tmhos/cm) 241 264 260 268 246 249 248 Temperature ('C) , 24.9 . 24.6 24.7 24.5 24.8 24.4 24.8 24.3 24.7 24.4 24.9 24.5 25.0 24.: pH (SU) 7.51 7.56 7.57 7.63 7.74 7.85 8.11 7.78 8.03 7.78 8.14 8.06 8.141 8.1: DO (myg/L) 8.2 7.6 . 7.7 . 7.6 8.2 7.9 7.7 6.9 8.1 7.0 8.1 7.9 8.11 7.: Conductivity (pLmhoslcm) 220 241 237 250 225229235 100% Alkalinity (mg/L CaCO3) 68 72M76 Hardness (mg/L CaCO 3) 86 78 98 Total Residual Chlorine (mg/L) <0.*1 <0.10 <0.10 Temperature ('C) 25.0 24.7 24.8 . .. 24.9 24.3 24.8 24.6 24.7 24.4 24.8 24.4 25.0 24. pH (SU) 7.481 7.58 7.61 7.63 7.75 7.86 8.09 7.79 7.95 7.76 .8.09 8.03 8.13 .. 0 DO (.g/L) 8.41 7.6 7.71 7.7 8.1 7.91 .77 8.11 6.9 8.2 7.7 8.1 7. Conductivity (ttmhos/cm) 220 251 237 249 222-23 100% Intake Alkalinity (mg/L CaCO 3 ) 70 74 Hardness (mg/L CaCO 1) 8 88 94 Total Residual Chlorine (mg/I) <0.10 <0.10 <0.10 T'emperature ('C) 25.01 24.51 24.8 24.7 24.9 24.5 24.8 24.4 24.71 2 24.9 24.4 24.9 24.

Inc. Pag,- f6 Environmental Testing Solutions, Species: Pimeph ales promelas Date: kI- CA- C7', Client: TVA / Sequoyah Nuclear Plant - Non-treated L'U;hY I'l ,Iqtv Day 1" T 0 I 22 Analyst i, L1YL-Concentration Parameter .. . . *.

CONTROL _pH ('41) . - .___ ___.

DO (mr2/L) ... (ýimh scm)______ 1A .;1 Alkalinity (mg CaCO 3/L). Si i Hardness _ _ _ _ Temperatur'e (C) -LA - 1 tq 'S tq 1.0__ __q__ ZIA to PH (S.U.) _ __ ?__ _ __ 7___ *4 11.3% DO (mg/L) u_ ConductivityOva (_Tmhos/cm) .L 1_.. '4 ' _ __ . Tem._=_.perat u re ('C) %4.,-S LA*So -Lg-4 ,1"L * . IAII (S.U.) 9.T3)Z 2C)~yL) CJ -- !:k,,M.*( -140 22.6% DO (mqiL) ',)9 ,q - 57 Conductivity IL ( 1 imhos/m . 041 ____ .AY A-- Temperature ('C) Aq-1` 14.6 9 9 Ii 9 -- - 9 .,- ~ - pH (S.U.) _9. f57~ ýU.,.0 45.2% DO (mgrL) q i ,7)I_ Conductivity q 4. , (l4mhos/cm) * ,,

                                     !emperature ('C)

T______ 'LA ? LMI.

                                                                                                -4 13                                     Z.I/-,A 4                    . I           LAA pH (S.U.)                                  i§-       q,0                                                             j                           Xl)5o
                                                                          .__._           ..       ..:                                        .               (..

72.6% DO (mgiL) Conductivity ,2-

                                   *-l(rmhos/cm)

Temperature ('C) 114.*TC 1 "_. *.. _

V.4 _L4 P*H (s.u.) -1.-15 100% DO (mg/L) .-

Conductivity (*.mhos/cm) Alkalinity (mg CaCO 3/L) Hardness (mg CaCO 3/L) TR chlorine 0,0 (mg/L) Temperature( (.Q0 0 C) N -.1. z . 2, pH (S. U.) '.,1 "7.).5 .U I -. (P

                                                                                                                                        "-     O._5      ')..),y                      ,

100% Intake DO (mg/L) 4:,,14 Conductivity (iimhos/cm) 9L5 Alkalinity (ing CaCO 3 /L) Hardness . . (mg CaCo-/L) TR chlorine (Mg/L) Temperature (OC) 2Sc.0 "q.. .q. 2 . . Zq." Initial .11 Final I I Initial 11 Final Page 35 of 101

Environmental Testing Solutions, Inc. Page 6 of'6 Species: Pinmephalespromelas Date: _ Client: TVA / Sequoyah Nuclear Plant - Non-treated Day 3 4 55 6 AnlI Concen- Parameter M,

                                                                                   %l       '014 CONTROL         F. ( U.).U p(s.u.)                                                     -                 .ro         " 9-      &o DO L(ngL)                  ).

Conductivity ,. (ýmhos/cmn) .3i" L&2L Alkalinity (Mg CaCO 3/L") (mg aCO3 /L) .4 A Temperature(°C)('Q ")..q 1".q- , .A.\ - -, PH (S.U.) ,-L %' ' 'Vq,I k i. _. o-A. 11.3% DO.(m) L) Conductivity (,Lmhos/cm) ;..** Temperature ('C) -LA-S ".L-S 7A VA- -L , 22.6% DO (rn L)- j*r Conductivity 20 ,. "' Conductivity .,-. - LJT}. (Vmhos/cm) 0 Ternperature (' Q I-A1 -LA.c, ~ i. .~I -1452'. 72.6% DO (ma/L) PH(mg CaC.) -- s Conductivity (ý hos/co) -I' 2q ., .Z _2.62q~ V.I. A- $i " .$0 q."

             , "Tern)erature (°C)

Temperature('C) A-(o . *& _ . S Z MI2.".7 100% DOI(mgiL) Hardness F. Fain (mg CaCOfL) TRchlorine (m /L) Z .O. TR Chlorine (mg/L) zo Ternperature ('C) 2 q44 1Fia q. IitaFnl 74A124 nt a ia PHiia Iiia inl Page 36 of 101

Page I of 6 Environmefntal Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

                                                        .Species: Pimephales.promelas Client: TVA                                                                            County: Hamilton Facility: Sequoyah Nuclear Plant                                                      Treatment: UV-treated NPDES 9: TN 0026450                                                                    Outfall: 101 Project#: 3SS\

Dilution reparationinformation: Comments: Diluto- prep (%) 11.3 22.6 45.2 72.6 100 Each concentration was treated Etfluent volume (mL) 282.5 339 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2217.5 21.61 1370 685 0 to remove pathogenic Total volume (mL) 2500: 2500 2500 2500 2500 interferences. Test organism information: Test information: Organism age: 2Db. O*kS5 0*C,_ Randomizing template: gLwe Date and times organisms - I*OO Incubator number: were born between: Organism source: -iPr Sf ýxL-Oý.-0210 Artemia lot number: I*O-ZCA-j Transfer bowl information: pH" Temperature = 'C Total drying time: 7.,,Aao-% 1-k -L- Date / Time in: .1 jmL5O Average transfer volume: Date / Time out: rL4tL.0, _'_ISO Oven temperature: Dailyfeeding and renewal information: Day Date Morning Afternoon Test initiation, MHS Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 - _ 0 -- t0a \--- A ,o1-0 01 2 o j---t4o *q %LAk: Iv5 0- 01-0n 6 C-I 12 0S.7.Z i -z s 2 3 ' 0 k-' 1qoL *,*__ wl -01" 0 O"! k - 7- -1 L-5 6 IlI 061 OcYt154 tko0q tiL' \ t-1

                                                                                                 --      *1*

(A %*l ai L4I, r Control information: Acceptance criteria Summary of test endpoints:

             % Mortality:                                                              < 20%                 7-day LC5 0            > 11O7.

Average weight per initial larvae: o. "1t NOEC I OQ Average weight per surviving larvae: 0 .1 !,A- > 0.25 mg/larvae LOEC "> I Y ChV > tOO7 I> t 07, Page 37 of 101

Page 2 of 6 Environmental Testing Solutions, Inc, Species: Pimeph ales prom elas Date: t%-C"" Client: TVA / Sequoyah Nuclear Plant - LW'-treated Survival and Growth Data Day CO.NTROL 11.3% 22.6% A B C D E F G 1H1 l31 K L 0 J0 ) 1C t (0 io t0 /0 /0 / lbD /C /C Q11o /0 /0 /0 '0 to 00 /0 lb '0 tO 2 t0 10 1C0 1I .D I0Db I0 lo ID to

                                                         /0     10 Il         /0     /0      /0        /       I C)     /C)0 CO '0 4
                                              /Q        t(o    to         to      ,0       0.      I
                                                                                                   /0          I0      /C        /0            /0 1        /        b0 /o IC-                  to /0             0 to         /I         to     to 4

6 1 o0 ' . 10 I'C0 '. C) /0 / 3 ( ) /0) 7 tt.*. L t** f 00 IC 9 10 t tC ( / f0 0O0 A Pan weight (mg) Color identification: C , to, t t3 .00 t4,S'ý N .1% 13 .84 t3-13 t', 1q.3-1 Iq.7$ q .C 1 .* kj.t$ 55 4a Analyst: LA4( B = Pan + Larvae weight (mg) Analyst: L 19ý.% i*.* i.9 . a,3O.5, I¶---aa q at.51 aa,eS ai.3 ,o.61 at.g Larvae weight (mg) =A -B Weight per initial number of larvae (mg)

           = C / Initial number of larvae  0'              \                             Q0       '"              0"
                                                                                                                              '0     t*

Average Percent weight per reduction initial from control 0.1 0.1.7 number of % larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, Ig unusually large, .d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 38 of 101

Environmental Testing Solutions, Inc. Pace 1of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: \.-cA'\ Survival and Growth Data Day 45.2% 72.6% 100% _______ Q Ro' S U V x 0 ~__ O li O t[0 /0 /0 C(. /y /_JO /0

                                                /0                              /0   /0to       /. 00 /c           !0         /U     t /c          /0       /© 21                10           1CO        it      to   IO   U     I OCO o                        I0       /O         t
                              .2)                          .10        tO       10     10        lb 10                  -0      /0       /0         /0       /0 31                        10       (10 to0o l()                          C)      /0                  /o         /0)       /D 4                    0         to       /0     /0       /0         /0       /0         'C>       /0        'o         '0      /C)
                                                 /U         /I       /c       10      /0         lo       to        '0         /0      /0           /o      /0 (0)6          0       10      I1       to      (0         t0        t(         /0        io io              /0 7

it to (C tO (I t(U 0 1C 0C) t C) '/

                                                     -it      -

A = Pan weight (mg) Color identification: (40_rk\~ OVA% K-t1.. .*P&N-04 IS-.11 1,'1-9 tq~0A00*IS'-*

                                                                                                                   *,8                           *f.. q.,t*

Analyst: B = Pan + Larvae weight z.3 .1a -sa.P0 w a Analyst: Larvae weight (mg) = A - B 16*j t,.SR' 5.101 66 o IAl lo, 1. 6. (o.S4 O 6 '.012.,7 (e.'4 Weight per initial number of larvae (mg) e C / Initial number of larvae b" :*"

O" O"*. c" e," O.

O"" t' Average Peicent weight per reduction -. initial from control 0. e 4.1 &Z 1 O' .JtSO number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m m issing, A sk sick, smn unusually small, ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 39 of 11U

Page 4 of 6 Environmental Testing Solutions, Inc.. Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: r?"- A-(r-Survival and Growth Data Day 100% Intake Y Z AA BB

                                                                   *t              0
                                                                                                 ')0      ~0 2

4 to (00 /0 t0 10O 10 tO (0 tO (0 10 A Pan weight (mg) Color identification: Analyst: L" B = Pan + Larvae weight (mg) A.13 to ?a .,xOqt at.ll. Analyst: _ _ Larvae weight (mg) = A- B Weight per initial number of larvae (mg) A C / Initial number of larvae " " 0*- Average Percent weight per reduction initial from control 0. 10l number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, go= unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: I Page 40 of 10

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated December 04-11, 2007

C 0 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 3551 Reveiwed by: fe *"trt /L 44-4 .--- . r Not for Complia nce Assessment, Internal Laboratory QC -- Concentration (% ) Replicate Initial ou of mber Final number of A - Pan weight B =Pan,+Larvae Larvae weight (rog) W eight uI clo. a ght. C W eightloitiel no otb Mean urvi Mean weightI Coeficdienrof Percent rea*ctionl Icrn larv a e lI a r. ( igt) weig h t ( ) ag A B n tb er of larv ae (cog) S u rolvio g n um ber of (t11- .dah,In , *.0

1. , oflorv a (tmg) (04 ) I ciltal nu n ber of va riatio n c-*. , hltc con trol (%)

(n.ge larva I ,netr *tt n2(5) rv mo r.Itdotneef.....

                                                                                                                                                                                                                                                                                    ".-I     ..        l-)

A t0 0 13.00 19-82 6.82 0.682 0.682 Control 1 [0 9 14.39 19.95 5.56 0.618 0.734 13.8 0.556 97.5 0.719 17.6 Not applicable C 10 t0 14.78 23.08 8.30 0.830 0.830 10 1 10 13.84 21.92 8.08 0.808 0.808 E 10 9 13.43 19.52 6.09 0.677 0.609 11.3' F 10 10 13.38 20.02 6.64 0.664 0.688 3.8 0.664 97.5 0.671 7.2 6.7 G 10 10 14.37 21.24 6.87 0.687 0.687 tto 10 14.33 21.57 . 7.24 0.724 0.724 t10 10 14.50 22 .5 1 8.0 1 0 ,801 0 .80 I 226J 10 10 14.72 21.33 6.61 0.661 0.740 8.1 0.661 100.0 0.740 8.1 -2.9 K K0 10 12 13 .5 7 0 .8 7 7 .3 0 0 .7 30 0.73 0 L 10 10 14.21 21.88 7.67 0.767 0.767 M 10 10 14.48 21.64 7.16 0.716 0.716 45.2% N 10 10 14.66 21.25 6.59 0.659 0.685 (0.5 0.659 100.0 0.685 4.7 0 10 10 14.81 20.80 5.99 0.599 0.599 P 10 10 14.04 21.70 7.66 0.766 0.766 Q 10 10 13.97 20.89 6.92 0.692 0.692 7 2 .6 % T R to 10 10 I0 113.88 3 .9 0 20.7 8 6 .8 8 0 .6 88 0.0 3 8.7 0.6 8 8 20.31 6.43 S 10 10 14.60 22.49 7.89 ý0.643 0.789 0.789 0.643-10%V 10 10 (4.42 20.45 U 10 10 12.61 19.11 6.03 6.50 0.650 0.603 0.6509100.0 0.703 8.7 2.2 _ _ _ _ _ _ __ _ _ _ _ _ _ _07 0.650 6.3 0.603 100.0 0.680 6.3 W (0 t0 14.58 21.60 . 7.02 0.702 9.7

                                                                                                                                                                                                                                   -0.702 X                  10                 (0                14.16                20.59                 6.43                   0.643                                                                          0.643 AA                  1(0 to                (0 10                15.85                22.76                  6.91                  0.691                                                                          0.691 13.60                20.96 I BB       I                                                                                            7.36                  0!736 180%yinttake           Z                 (0 1t               10 (0                14.81 14  .99      1       221.92

1. _97 . 7.11 6.98 0.711 . 0.709 2.8 0.7(11 0 .736 o .0 . .

0 .698 1 0.698111 Outfall 101: MSD = Minimum Significant Difference Dunnett's MSD value: 0.1256 PMSD= Percent PMSD isMinimum a measureSignificant Difference of test precision. The PMSD is the mninimumrpercent difference between the control and treatment that can be declared statistically significant in a wholeeffluent toxicity test. PMSD: 17.5 Intake: Lower PMsD bound dleterarmned by USEPA (I0tit percentile) - 12%. Dunnetl's MSD value: 0.1246 Upper PMSD bound determined by USEPA Lower and upper PMSD bounds were (90th percentile) = 30%. PMSD: 17.3 dletenmined from the 10th and 90th percentile, respectively, of PMSD data fi'om EPA's WET Interlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001[b). USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic andAcute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appencdix. EPA-82I-B-01-004 and EPA-82 I-B-01.005. US Environmental Protection Agency, Cincinnati, ON.

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated December 04-11, 2007 0 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 12/4/2007 Test ID: PpPFCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 . Test Species: PP-Pimephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.6820 0.5560 0.8300 0.8080 11.3 0.6090 0.6640 0.6870 0.7240 22.6 0.8010 0.6610 0.7300 0.7670 45.2 0.7160 0.6590 0.5990 0.7660 72.6 0.6920 0.6880 0.7890 0.6430 100 0.6500 0.6030 0.7020 0.6430 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat . Critical MSD Mean N-Mean D-Control 0.7190 1.0000 0.7190 0.5560 0.8300 17.626 4 0.7190 1.0000 11.3 0.6710 0.9332 0.6710 0.6090 0.7240 7.177 4 0.921 2.410 0.1256 0.7054 0.9811 22.6 0.7398 1.0289 0.7398 0.6610 0.8010 8.107 4 -0.398 2.410 0.1256 0.7054 0.9811 45.2 0.6850 0.9527 0.6850 0.5990 0.7660 10.525 4 0.652 2.410 0.1256 0.6940 0.9652 72.6 0.7030 0.9777 0.7030 0.6430 0.7890 8.746. 4 0.307 2.410 0.1256 0.6940 0.9652

           .100        0.6495          0.9033         0.6495       0.6030        0.7020           6.261        4             1.333         2.410      0.1256       0.6495          0.9033 Auxiliary Tests                                                                                            Statistic                 Critical                   Skew            Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01)                                             0.97521883                   0.884                  -0.3876268     0.23957661 Bartlett's Test indicates equal variances (p = 0.45)                                                     4.72200441                15.0862722 Hypothesis Test (1-tail, 0.05)                   NOEC          LOEC           ChV             TU            MSDu        MSDp          MSB          MSE          F-Prob            df Dunnett's Test                                     100          >100                           1         0.12562972   0.1747284    0.00431004   0.00543476   0.56852084         5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point % SD 95% CL(Exp) Skew IC05 79.342 ICtO >100 IC15 >100 IC20 >100 IC25 >100 1C40 >100 IC50 >100 sqn_05-30-O7data

TVA I Sequoyah Nuclear Plant, Intake 4ý1 UV-treated Co December 04-11, 2007 0. Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 12/4/2007 Test ID: PpPFCR Sample ID: TVA / Sequoyah Nuclear Plant, Intake End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.6820 0.5560 0.8300 0.8080 100 0.6910 0.7110 0.7360 0.6980 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical* MSD Mean N-Mean D-Control 0.7190 1.0000 0.7190 0.5560 0.8300 17.626 4 0.7190 1.0000 100 0.7090 0.9861 0.7090 0.6910 0.7360 2.795 4 0.156 1.943 0.1246 0.7090 0.9861 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93012404 0.749 -0.7387113 1.46486826 F-Test indicates equal variances (p = 0.01) 40.8998299 47.4672279 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.12462388 0.17332945 0.0002 0.00822633 0.88120699 1, 6 Treatments vs D-Control Linear Interpolation (200 Resamples) Point % SD 95% CL(Exp) Skew IC05 >100 IC10 >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqnO5-30-O7data

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated 4ý- 4C December 04-11, 2007 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) 0 Species: Pimephalespromelas Daily Chemical Analyses Project number: 3551 Reviewed b Concentration Parameter Day 0 Da 1 Day 2 Da 3 Day 4 Day 5 Da 6 Initial Final Initial I Final Initial I Final Initial Final Initial I Final Initial Initial Final Final PH (SU) 7.62 7.55 7.611 7.58 7.671 7.86 7.99 7.68 8.021 7.71 8.08 7.92 8.01 7,98 DO (mg/L) 8.0 7.7 .7.71 7.9 7.91 7.7 7.6 7.2 7.71 7.1 7.91 7.8 8.01 7.6 Conductivity (4mhos/cm) 295 .3316 31* 333 Control 294I 306 33 Alkalinity (mg/L CaCO 3) 57I58 57 Hardness (mg/L CaC0 3) 88 92 88 Temperature (°C) 24.8 24.7 24.7 24.6 24.8- 24.6 24.6 24.6 24.7 247 . 24.6 24.3 24.8 24.4 PH (SU) 7.61 753 7.61 7.57 7.68 7.80 8.04 7.63 8.01 7.69 8.08 7.89 8.05 7.97 DO (mg/L) 7.9 7.9 7.7 7.9 .7.8 7.7 7.7 7.2 7.7[ 7.0 7.9 7.7 7.9 76 Conductivity (1imhos/cm) 288 319 315 329 t 295 i 301 319 Temperature (°C) 24.9 24.5 24.8 24.4 ..24.8 24.2 24.8 24.3 . 24.7 24.5 24.8 24.3 24.9 24.6 pH (SU1) 7.60 7.53 7.61 7.58 7.61 7.85 8.05 7.62 7.99 7.79 8.10 7.95 8.06 8.00 DO (mgIL) 7.9 7.7 7.7 7.9 7.81 7.6 7.7 6.8 22.6%/o Conductivity (,umhos/cm) 7.71 6.9 7.91 7.6, 7.9 76 281 308 .3050 311 277 . 293 ' 291 Temperature (°C) 24.9 24.9 24.4 24.91 24.2 24.8. 24.4 24.7 24.6 24.8 24.1 25.0 24.6 pH (SU) 755 7.61 7.59 7.63 7.85 8.08 7.62 7.99 7.78 . . 8.12 7.99 .8.09 . 8.03 DO (rg/L) 7.9 7.6 8.0 7.9 7.6 7.8 6.6 7.8 7.1 7.9 7.5 7.9 7.7 45.2% Conductivity (pImhos/cm)

259 294 286 3o 305 260 275 285 Temperature (°C) 24.9 24.8 24.9 24.7 25.0 24.3 24.9 24.2 24.7 24..8 24.5 25.0 24.3 pH (SU) 7.55 7.55 7.61 7.56 7.62 7.90 8.09 7.83 7.99

                                                                                                                     .                                           7.82             8.15

8.02 8.12 8.05 DO (mg/L). 8.0 7.7 7.8 8.0 8.1 *7.7 7.8 6.6 7.9 7.0 81) .7.6 7.8 7.7 72.60/a Conductivity (jimhos/cm) 236 266 261 - 278 239 253 258 Temperature ('C) 25.0 24.8 24,9 24.6 25.0 24.3 24.9 24.1 24.7 24.2 24.9 24.2 25.1 24.5 pH (SU) 7.52 7.61 7.62 7.57 7.67 7.90 8.12, 7.76. 8.001 7.821 8.18, 8.061 8.&15 8.09 DO (mg/L) 8.1 7 . 7.8 8.0 8.1 7.8 7.9 6.81 8.21 7.1 8. 7.6[. 7.8 7.7 Conductivity (limhos/cm) 219 245 241 1000/a 220 232 237 Alkalinity (mg/L CaCO 3) 72 661 70 Hardness (mg/L CaCO 3 ) 90 94 86 Temperature ('C) 25.1 .246 25,0 25.11 24.21 24.9 24.1 1. 24.8 24.5 24.9 24.25 24.5 pH (SU) 7.521 759 7.64 7.60 7.65 7.861 8.12 7.771 7.981 7.85 8.131 8.04 8.16 8.08 DO (mg/L) 8.3 7.8 7.71 8.21 8.16 7.81 9 6.9 8.3 7.1 8.2 7.7 7.8 7.7 Conductivity (ý.smhos/crn) 217 27237 237252326 100% Intake Alkalinity. (mg/L CaCO 3 ) -6 70 Hardness (mg/L CaCO])
8 889 Temperature (0 C) I 25.0 24.6 24.8 24.41 25.01 24.6 249 24.2 24.8 24.2 . 24.9 24.2 24.8 24.6

Page 5 of 6 Testing Solutions, Inc. I Environmental Species: Pimephalespromelas Date: i .-CA - G') Client: TVA / Sequoyah Nuclear Plant- UV-treated DailyC Ii Day = I 9 I 0 II ,. I 1 II I 2 I Analyst I P' Concentration I Parameter CONTROL PH (S.U.) DO (mg/L) Conductivity (urmhos/cm)

                                                ,,{    ,                -

i-.S l1.W 'A

I .* zA't.

Temerature ("C) I 4-pH (S.U.) 11.3% DO (mg/L) za~q II-L,*.S IN:,* I 7.,t~ I LA- II L -t Conductivity (jmhos/cm) Temperature ('C) pH (S.U.) - 22 .6% DO (mgIL) ( Conductivity Temperature (°C) TA.9 '..iA-

                                                                                                                         . .                           "'-L--

pH (S.U.) I O.Z3 5s II7* 9tl II 45.2% DO (mg/L) 7C Conductivity 15q (prmhos/cm) _________ Temperature ('QC) ~ - 2i .Z 2.9'. 1 pH.(S.U.) 1-0 .) ,/=(0~ ).C 72.6% DO (mgrL) 13._ Conductivity (prnhos/cm) _______Temperature (C) 150 7qj. 1.. .0. ApH ( Z),.,. I ,II 100% I%~LI k- ) Conductivity (pmrhos/cm) IAr A4 Temperature ('C) iSA fl PH (S.U.) t

                                                                                                         "*..3                 01 I1 1 14,         11o-aS

I.,

100% Intake jDO (mgfL) Conductivity (jimhos/cm) I Temperature (°C) ZS.0 2zq. f IN-Lq "I,* Initial 11 aI L Initial 11 Final I Initial II Final I 0C)a r" A1 C,7a IC: C .

                                                                            -.7 Page 45 of 101

I Environmental Testing Solutions, Inc. Page 6 of 6 Species: Pimephales promelas Date: \'A--cr-Client: TVA / Sequoyah Nuclear Plant - UV-treated Dav 4 5 6 Anal st " P Concen- Parameter* tration CONTROL-----.--' . a 1... e.op '. DO (mg/L) Conductivity 13 0 (ýi mos/m) Temperature ('C) te,-2.0- "/' " * :,..*-.a PH (S.U.) r~q-70 11.3% DO (mg[L) Conductivity

                     &tmhos/cm)

Temperature(°C).Q "A., Z b,- 1 -* -, L a,. .' " . .(, pH (S.U.) ( (.0 22.6% DO (mgfL) Conductivity O(PM oS/cM) Temperature(°C) (_Q "114 -1 152 45.2% DO (gL) l L Conductivity (4mrhos/cm). 0 14.9F. " L. O_ Temperature (°C) pH (S.U.) el7 72.6% DO (mZ , , 0 Conductivity25 _______ Ternperature ('C) 24 A ~*.. 4 100% DO(mg[L) 14-(4 -ills Conductivity

Temperature ('C) . -L .* "lL1- - *t L5 (mhos/cm) " C) 930*.
100% DO (.r..L) . ..

Intake ConductivityUSkc (4mhos/cm) ______.1 Temperature (0 C 4'Q2.15 ~i. ~ 1 Initial U Final I Initial If iInnat n al I flrLa, Initial II Final I hX\14.

                                                                                         ~L..

7cz rage 46 of 1 0 1~ ~JZ.

Environmental Testingo Solutions, Inc. Paýýe I of .7 Environmental Testing Solutions, Inc. Page 1 of? Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphniadubia Client: TVA County: Hamilton Facility: Sequovah Nuclear Plant Treatment: Non-treated NPDES #: TN 0026450 Outfall: 101 Project#: ms*-z;S Dilutionpreparationinformation: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 339 1130 1815 . 2500 Diluent volume (mL) 2217.5 2161 1370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information: Organism age: 4 VA- A oQ.VLG o,..t) Randomizing template: 5)A,0 Date and times organisms 'L.-oa.-"L1 01'wL. -To -oO .Incubator number and 2..7-were born between: shelf location: Organism Source Cups: - - YCT batch: Culture board: W-1---1 A 61.*46*,4,6o 12 1'%1 Ito, 1-Transfer bowl information: pH =".&E Temperature = -8. R Selenastrum batch: i\--Cfl Daily renewal information: Day Date Test initiation, Control water Sample numbers Analyst renewal, or batch used used termination time

fl-ds-cr I 00(: Vz-O*-*A, cnz's. rsog 4
3 tz*--' OO'L_ !.-- t~n~ oaz*'_t24Z.'

(' Control information: 1 2 Acceptance criteria Summary of test end oints:

           %ofMaleAdults:                                          7.              *20%                 7-day
                                                                                                           .1I%'

LCQ0

           % Adults having 3rd Broods:

bc 7 , oo07. >80% NOEC I w7__.

           % Mortality:                              67.         0                  _ 20%               LOEC                  lz1r575 Mean OffspringfFemale:                             33%,.%. _ 15.0 offspring/female       ChV               > UO7
           % CV:                                   -1. 177. 6 ."17.           < 40.0 %
                                                                                 <                      1C25               ) 1007.

Page 47 of 101

Environmental Testing Solutions, Inc. Page 2 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: - CONTROL - I Survival and Reproduction Data Replicate number Dav 1 2 3 4 5 6 7 8 9 10 S]Youngproduced __ ©1e1 \l i ] ! iJ* - E___tm:- I--t___ _____ a) ___ C_ _ -- _ _-- I __ Adul mo tlt

                              ! dult mortality 1- - i I _
                                                          .             1      l-"                   ..

_] _ _ ___ _._ I[ .___ Young produced .___ __ _ o C) C c* 6 Young produced Adult mortality _

                                                              . C
                                                                         -1I-!*i c          C                 )

t--I--I\--I*-- 4 Young produced LI A 'I AI A- I %A A Total young produced _ _ c_ _ Final Adult mortality 9.. _ .1o6 j _. _L.' I - kI D ý a% ) s2 3 -S_____. -. ' A Note: Adult mortality Xfor un prood 7(Lucedlive, D c e)_Concentration: on~rdcdI Conentration:- 1 0 -I) C1 - IL

                                                                                                                           %Mortality:                                              o7.

Mean Offspring /Female: 3ý .0% CONC: 11.3% Survival and Reproduction Data I Day____ 1 Youngproduced I Da_____ 1 0 213 __Replicate c 0 4 C S] number _ 0 6 7 0 C 8 9 0 10 I 2 Young produced Adult mortality _ .'L___ L*

                                                                                           ~..___
                                                                                                      -                -     L---                           L-             s--

T Young produced

                         ]Adultmortality C) t 0

U[ \K-I 0 I _I_\. I I 0

                                                                                                                                                                          \---

4 Young produced %A LA '© VWI4 _ Adult IAdult mortality I mortality I l '-%A ' -- IV---I L I L --t1L._I L{

                                                                                                                                           .         _ U_..
                                                                                                                                                             \ -]         '--_]             _

5 Young produced [2I 'L2I 3 13 1L I 12* 2_ I 61 6 Adult mortality____ __ __ __ ________ mortality ][t. [ AdultYoungproduced ) C)O o

                                                                                                      . I             )       0))
                                                                                                                                 --                       L          I o-                  _

7 ý Young produced is 19 1 -1 0 tIko I ,,o CiI' l s -1 Total young produced 3 3 3 -"I 3 I3 -1 3 331 ,3 Z 31 52 Final Adult Mortalit L KI. ] J k -. , , Zi.

                                                                                                                                 -I                   -         ._                L        .

Note: Adult mortality (L = live, D dead) Concentration:

                                                                                                                          % Mortality:                                          .

Mean Offspring/Female: 33.S I%Reduction from Control:I - S .0 I Page 48 of 101

Environmental T'esting Solutions, Inc. Pag-e. " of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: , - coNc: 22.6% Survival and Reproduction Data Replicate number I Day _3 1 2 j _ - 6o

62. 7 8 9

                          ]Z                            I'O                                                                                            Ie dult mortality 7__

j_.. .1 __ _ - _ i 1 u2grdue O O b I-©___ O I Young produced Adult mortality Adul i t I- ý- 1

t °L Young produced ___ ___ ___ ____ 7 ____ ) S C*

I SAdult mortal,,t,, [ --J *IL- -.- J_L.- I L-- L__-_ ! I oe Adult mo rtality (,lie Adult mortality*' D.dead) TtlYoung produced a I Nt: Adult mortality( YounConcncedrAtin ie .ed I

                                                                                                       % Mortality:                                  07. 1 TtMean Offspring/Female                    y3-.      &
                                                                                                       % Reduction from Control:                  -      .17.

I CONC: 45.2% Survival and Reproduction Data Replicate number I Day 1 2 3 4 5 6 7 8 9 10 Young Y produced 0 C _ _ 01 0 0 0 0 2 ::** Adult mortality Yougtrodce 1 1_o o_ 01 - - _ I10 Iof ° __ Ic 3 Young produced 1Adult mortality [ c) j LI 1---IL1 0] __I __ I 5 Young produced 1 CT) Adult mty J U J , \-jt.... 7 Young produced .1,1 7 ZO 1 8 1 7.

Tf l, Totalyoung produced rS
3. 3.__ 35. . 3. .- I ._

o0 _ Final Adult Mortality - ~ -- C C - ~ ~ Note: Adult mortality (L = live, D = dead) Concentration:

                                                                                                      % Mortality:                                    )7.

Mean Offspring/Female: 3,.,

                                                                                                      % Reduction from Control:                   I . 7.

I Page 49 of 101

Environmental Testing Solutions, Inc. Page 4 of 7 Species: Ceriodaphniadubia Client: Suequovah Nuclear Plant - Non-treated Date: _-A-c-\ crypt 72.6% Survival and Reproduction Data

                                                                                ,___...         ___           Replicate number Day                                                    2            3       [       4           5         6         7            8             9          10 Voungproduced Y                                                       -             -)                                     7c                            0 Adult mortality                                                                                    _____._

Young produced 0 0 CQ)j______ __ __ Adult mortality Adult mnortality iL k . j II ._.I_. .. _ .* k _ . Young produced IL I 01 i I L-3i, t*Tc' J 6[YoungAdult produced mortality 1ILA0+/-AC.(i .- 3)3I$ v Ii_. I_._. k _-\ I___ -- I ._ I. Adult mortality_, L f J _ 7 Young produce d o iJL 2z C) I I Total young produced 3% -56 '4 0 s N 0 ! a ' Final Adult N-ortality I Vk7\. . L ~ L Note: Adult mortality (L = live, D = dead) Concentration:

                                                                                                                      % Mortality:                                   C7.

Mean Offspring/Female: S11.I

                                                                                                                      % Reduction from Control:                    -

CONC: 100% Survival and ReproductionData Replicate number Day 1 2 3 4 5 6 7 8 9 10 2 jYoun produced 1/21C 2 C1 ]& I 0313 10 _.

                            " Adult mortality      I         ..       \_.,           ,...               {      k.          L...      LI             .            .          _

4 : Young produced _ _ Adult mortality 6 _ _ t Young unp produced Adult mortality mortality duAdult [IA [ _ fik..._1_... L

                                                                                                *I..-

7._ I [ "-Af~i I 0 13

                                                                                                                                              .. [.

Ji___ 1 YYoung produced fl 1Si" 16 is"* is Total young produced p3, c'- ,3 __ p .* i 31 * , 1 Final Adult Mortalit y . U _ * *- .. Note: Adult mortality (L = live, D dead) Concentration: " u% Mortality: m Mean Offspring/Female: 2. I [Reduction from Control: - 20.,, IPage 50 of 101

Page 5 of 7 Environmental Testing Solutions, Inc. Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: I, -Lo-OLA-c CONTROL -2 Survival and Reproduction Data Replicate number Da _ 2 3 1 4 5 6 1 7 8 9 10 2 Young produced-Young produced 01- _ 1* Adult mortality . . . . 2 Young produced c) aTCo_.c-Note: Adult mortality D__e de ) Cnnproduced q 4tati mortality J0. ldult IL fnm eYoungproduced \t7 o Youngproduced Cr C i ad C5 =biDat Adult mortality \,- %,- %, Ib 7 Young produced 31 IS 1iS I L -1 15 1to Total young produced 30 3o-0 3~a 2ý a63 3~ 31 a Final Adult Mortality t.. L '_ - - * \' - Note: Adult mortality (L Damrtlyt L live, D dead)--- 2 3- _ _5 6_ 7_ _9 _ 3oConcentration: Mean Offspring/Female: CONC: 100% Intake Survival andeproductionData 1 Replicat number Day fi 2 3 4 5 6 7 8 9 10 Adult mortality - J --- L- - ._J_ __ t4 2] Y oung produced To Young produced c ' C7) 0 c) '4 -- I C - Adult mortalityil4_ t L - U.._ "Z. 3 Young produced ") L [j L L 1 U Y oung mortalit Adu Y lt producedy LA L I 'A ' I ,.*" _ _ 1_ _ _1 , i Adult mortality I.. 5 Young produced __I'A 14..I~j7. 'I7..~[1. Adult mortality ( Iv k dea-) L=

                   .6        Young produced         Ip)oetrti Adult mortalitt 7      [nYoung produced              z                                                 Ozae:                                             .

Total young produced 39 3-b 5 1 Z '31-3t -- 3 3% a-i a Note: Adult mortality (L =live, D = dead) Concentration:

                                                                                                               % Mortality:                            1Q7, IMean Offspring/Female:                  13 .
                                                                                                               % Reduction from Control: I - t                    %7.1 I Page 51 of 101

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated U1I 0~r December 04-11, 2007 0 Verification of CeriodaphniaReproduction Totals Control-i 72.6% 7D.a% Replicate number - Day Replicate numbrr Total Day 1 2 3 4 5 6 7 8 9 0 Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 .0 0 0 0 0 4 4 4 4 6 4 6 4 4 4. 4 44 4 5 5 4 4 5 4 4 6 6.4 47 5 12 14 13 11 10 12 13 15 11 12 123 5 14 14 16 13 13 15 14 13 16 13 141 6 L 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 00 0 0 0 0 7 15 14 18 116 13 16 114 15 17 14 1-52 7 1 20 18 18 20 17 19 16 19 183 Total 31 32 35 33 27 34 31 34 32 30 319 Total 38 36 40 35 36 39 35 38 38 36 371 11.3% - 1000/0 D Replicate 1lMnber Total Day '"i' Replicate number Total 4 5 6 8 9 10 1 2 3 4 5 6 7 8 19 1 T0 1 0 0 0 0: 0 0 0 0 0 0 0 1 0 0 0. 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0. 0 0 0 0 0

3. 0 0 0 0 0 0 0' 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 5 4 4 4 4 4 4 4 42 4 6 4 4 5 5 5 4 4 4 6 47 5 13, 14 12 13 13 10 14 12. 12 11 124 5 15 14 16 14 14 16 15 13 15 15 147 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 15 19 16 17 20 11 66 17 15 18 169 7 17 18 21 19 19 20 17 21 18 20 190 Total 33 37 33 34 37 30 34 33 31 33 335 Total 38 36 41 38 38 41 36 38 37 41 384 22.6% Control-2 Day Replicate number Day. Replicate number . Total 1 2 3 4 5 6 7 8 Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0. 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 5 4 5 5 4 4 4 4 44 4 4 3 4 4 4 .4 5 4 '4 4 40 5 13 12 14 14 13 15 13 11 14 13 132 5 12 12 11 10 10 12 13 10 12 11 113 6 0 0 0 0 0 0 0 0 0 0 6 0 0. 0 0 0 0 0 0 0 0 0 T7 16 19 19 16 15 18 18 19 172 7 14 14 15 17 15 17 18 17 15 16 158 Total 35 31 35 37 37 36 32 33 36 36 348 Total 30 29 30 31 29 33 36 31 31 31 311 45.2% 1000/%Intake Replicate nunber Total Replicate number Day 1 2 - 3 4 5 6 7 8 1 9 10 Dy 1 2 13 .14 5 6 1 7 9 10 Total 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0. 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 6 4 5 5 4 4 5 5 46 4 4 4 5 4 4 4 3 5 5 3 41 5 13" 15 12 15 13 12 15 16 13 12 136 5 14 11 12 14 12 11 12 14 12 124 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 1720 17 18 17 20 18 16 179 7 21.18 20 19 17 21 18 19 203 Total 34 38 35 39 35 35 36 40 36 33 361 Total 39 33 37 37 33 36 33 38 37 35 358

TVA / Sequoyah Nuclear Plant, Outfall 101 (I. Non-treated 0 December 04-11,.2007

  -5 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)

Species: Ceriodaphniadubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 3551 Reveiwed by: 4

A Concentration .. ..... Replicate number Survival Average reproduction Coefficient of M Percent reduction froin 1 2 3 4 5 6 7 8 9 10 (O) (offspring/female) variation pooled controls Control - 1 31 32 35 33 27 34 31 34 32 30 100 31.9 .7.3 Not applicable 11.3% 33 37 33 34 37 30 34 33 31 33 100 33.5 6.6 -5.0 22.6% 35 31 35 37 37 36 32 33 36 36 100 34.8 6.0 -9.1 45.2% 34 38 35 39 35 35 36 40 36 33 100 36.1 6.2 -13.2 72.6% 38 36 40 35 36 39 35 38 38 36 100 37.1 4.7 -16.3 100% 38 36 .41 38 38 41 36 38 37 41 100 38.4 5.1 -20.4 Control - 2 30 29 30 31 29 33 36 31 31 31 100 31.1 6.7 Not applicable 100% Intake 39 33 37 37 33 36 33 38 37 35 100 35.8 6.1 -15.1 Outfall 101: MSD = Minimum Significant Difference Dunnett's MSD value: 2.152 PMSD = Percent Minimum Significant Difference PMSD: 6.7 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Intake: Dunnett's MSD value: 1.660 Lower PMSD bound determined by USEPA (10 h percentile) =. 13%. PMSD: 5.3 Upper PMSD bound determined by USEPA ( 9 0th percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 2001a, 200tb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2-Appendix. EPA-821--B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

CJD TVA / Sequoyah Nuclear Plant, Outfall 101 Cm Non-treated December 04-11, 2007 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 12/4/2007 Test ID: CdFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 31.000 32.000 35.000 33.000 27.000 34.000 31.000 34.000 32.000 30.000 11.3 33.000 37.000 33.000 34.000 37.000 30.000 34.000 33.000 .31.000 33.000

           .22.6         35.000         31.000          35.000       37.000        37.000         36.000          32.000      33.000        36.000        36.000 45.2        34.000         38.000          35.000.      39.000        35.000          35.000         36.000      40.000        36,000         33.000 72.6        38.000         36.000          40.000       35.000        36.000          39.000         35.000      38.000        38.000         36.000 100        38.000         36.000          41.000       38.000        38.000          41.000         36.000      38.000        37.000      . 41.000 Transform: Untransformed                                         1-Tailed           I               Isotonic Conc-%           Mean         N-Mean           Mean           Min           Max           CV%               N         t-Stat       Critical       MSD          Mean         N-Mean D-Control         31.900         1.0000          31.900       27.000        35.000            7.307        10                                                   35.300          1.0000 11.3        33.500          1.0.502        33.500       30.000        37:000            6.638        10           -1.700         2.287       - 2.152      35.300          1.0000 22.6        34.800          1.0909         34.800        31.000       37.000            6.028.       10           -3.081         2.287         2.152      35.300          1.0000 45.2        36.100          1.1317          36.100       33.000       40.000            6.187        10           -4.462         2.287         2.152      35.300          1.0000 72.6        37.100          1.1630          37.100       35.000       40.000            4.660        10           -5.525         2.287         2.152      35.300          1.0000 100        38.400          1.2038          38.400       36.000       41.000             5.091       10           -6.906         2.287         2.152      35.300          1.0000 Auxiliary Tests                                                                                             Statistic                 Critical                    Skew            Kurt Kolmogorov D Test indicates normal distribution (p > 0.01)                                                0.61772913                   1.035                   -0.0536108     -0.5166972 Bartlett's Test indicates equal variances (p 0.96)                                                        0.98804921                15.0862722 Hypothesis Test (1-tail, 0.05)                    NOEC          LOEC           ChV             TU            MSDu        MSDp          MSB            MSE        F-Prob            df Dunnett's Test                                      100          >100                            1        2.15229383 .0.06747003       57.08    - 4.42962963     2.9E-08          5,54 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point % SD 95% CL Skew IC05 >100 IC10 >100 IC15 >100 IC20 >100 IC25 >100 sqn_]2-04-07data IC40 >100 IC50 >100

JIQ TVA / Sequoyah Nuclear Plant, Outfall 101 M Non-treated December 04-11, 2007 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 12/4/2007 Test ID: CdFRCR Sample ID: TVA/ Sequoyah Nuclear Plant, Intake End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 30.000 29.000 30.000 31000 29.000 33.000 36.000 31.000 . 31.000 31.000 100 39.000 33.000 37.000 37.000 33.000. . 36.000 33.000 38.000 37.000 35.000 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 31.100 1.0000 31.100 29.000 36.000 6.685 10 33.450 1.0000 100 35.800 1.1511 35.800 33.000 39.000 6.148 10 -4.909 1.734 1.660 33.450 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95055902 0.868 0.55299874 0.10323569 F-Test indicates equal variances (p = 0.87) 1.12082267 6.54108953 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic I Test indicates no significant differences 1.66023952 0.05338391 110.45 4.58333333 1.1E-04 1, 18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point %. SD 95% CL Skew IC05 >100 IC10 >100 IC15 >100 IC20 >100 1C25 >100 IC40 >100 sqnl 2-04-07data IC50 >100

1. TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11, 2007 0

  -i Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)

Species: Ceriodaphniadubia Daily Chemical Analyses Project number: 3551 Reviewed by: Concentration Parameter Da 0 . Day 1 Da 2 Day 3 Day 4 Da 5 Day 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final pH (SU) 7.83 7.58 7.381 7.66 7.83 8.00 8.06 8.02 8.10 8.07 8.15 7.99 8.02 8.06 DO (mg/L) 7.7 7.7 7.71 7.91 7.6 7.5 7.71 8.0 7.61 8.01 7.6. 8.1 7.4 7.6 Conductivity (1smhos/cmj 296 31 310 37304 30631 Control Alkalinity (mg/L CaCO3 ) 57 57 57 Hardness (mg /LCaCO 3) 92 94_92 Temperature (OC) 24.6 24.7 24.7' 24.9 24.7 24.5 24.7 24.6 24.6 24.8 24.8 24.6 24.6 24.9 pH (SU) 7.58 7.57 7.57 7.65 7.67 8.03 8.00 8.02 8.04 8.08 8.09 8.01 8.02 8.06 DO (mg/L)

8.0 7.7 7.6 7.9 7.9 7.5 7.4 7.8 8.0 8.0 7.8 8.1 8.2 7.6 Conductivity (pmhos/cm) 287 315 312 3. 293 296 302 Temperature (C) 24.7 24.9 24.7 24.6 24.7 24.5 24.7 24.8 24.7 24.8 24.7 24.9 24.8 24.9 pH(SU) 7.58 7.57 7.56 7.66 7.69 8.03 8.04 8.02 8.05 8.08 8.09 8.03 8.05 8.07 DO (mg/L) 8.0 7.8 7.6 7.9 7.9 7.5 . 6 7.8 7.9 7.9 7.8 8.2 7.9 . 7,7 22.6% Conductivity (jsmhos/cm) 280 305 304 320 .285 288 3003 Temperature ('C) 24.7 24.9 24.7 24.8 24.8 24.8 24.7 24.57 24.7 24.84 pH (SU) 7.58 7.56 7.56 : 7.67 7.70 8.03 8.07 8.06 8.05 809

                                                                                                                                                                                              &8.09        8.05          8.07          8.11 DO (mg/L)                                8.1            7.8             7.6          8.0  .         8.0         7.6         7.6             9         7.9                       8.0           8.2          8.0          7.6 45.20     Conductivity     (fimhos/cm)            263                            287                        281                      30                        267                       255                        280 Temperature (0C)                       24.7           25.0            24.7         24.8           24.8        24.7        24.8          24.5        24.7         24.6         24.7         25.0          24.98         246 pH (SU)                          . 7.54           7.57            7.57         7.69           7.71        8.07        8.08          8.07        8.04         8.16          8.13         8.10         8.09          8.12 DO (mg/L)                                8.1            7.8             7.7           8.0           8.2         7.7         7.6           7.9         8.0          8.0           8.0          8.2          8.2          7.6 72.6%     Conductivity (gmhos/cm)                 241                            264                         260                     268                       246                        249                       248 Temperature (C)                       24.8            24.8           24.8          24.9          24.8        24.5        24.8          24.6        24.7          24.9         24.7         25.0         25.1          24.8 pH (SU)                               7.511          7.57            7.57          7.75          7.741       8.00        8.11          8.12         8.03        8.16          8.14         8.141        8.14          8.19 DO (mg/L)                .8.21                           7.8,           7.7           8.0           8.21        7.8    . 7.71          8.0          8.11         8.11         8.1,         8.01         8.1           7.7 Conductivity (gmhos/cm)              .22011112411111111111111                                     237                     250                       222923 100%      Alkalinity (mglL CaCO 3)                 68                                                         72                                                 76 Hardness (mg/L CaCOj)                    86                                                         7898 Total Residual Chlorine (mg/L)       <0.10                                                      <0.10ý                                            <0.10 Temperature (C)                       25.11           25.0           24.81         24.9          24.9         24.81       24.81        24.6         24.91        24.6         24.7         24.81        25.1          25.01 pH (SU)                               7.481        . 7.621          7.611         7.74          7.75     . 8.061       8.091        8.12         7.951        8.181        8.09         8.121        8.13          8.211 DO (mg/L)                               8.4             7.6            7.7           80            8.1          7.6         7.7     .7.8              8.1          81           8.21         8.1,         8.1        . 7.8' Conductivity (tiinhos/cnm)             220                            251                    .237                          249                       222                       230                       223 1000/,, Intake  Alkalinity (mg/L CaCO 3)                 70                                                         74                                                 74 Hardness (mg/L CaCO 3)                    84                                                        88                                                 94 Total Residual Chlorine (i           <0.10                                                     <0

_Temperature(OC) . 25.0 24.71 24.8 j 25.0 24.9 24.7 24.8 .24.7 25.11 224. 24.7 24.9 24.8

V Inc. Paae 6of 7 Environmental Testing Solutions, r Species: CeriodaphlniaIdubia Date: vZ-oA- 4JI Client: Seauovah Nuclear Plant - Non-treated Daily Chemistrv: Day 0 I 2 Concentration Parameter 'U.4 ,M ' CONTROL pH (S.U.) _ _; _ ______.. _ ___ DO (rag/L) ' :")'*;-U". A.lkalinity (jmhos/cm) 4 N (rgCaCOd) *q." Hardness '*AZo0 (mg CaCO3/L) i Temperature (°C) "1A .1 "A .- ". L -- 1 I__-__ PH (s.u.) '- ,.c Su* 11.3% DO (mg/L) . YJ Conductivity - (ýtmhos/cm). Temperature (°C) "L." 'I " q,-

                                                                                                                    "AAt               W            -zA- 5 pH (S.U.)                                                                              c_0
                                                                                                                       ,.L(o                         ,.c3, 22.6%            DO (mg/L)                                                                        ".)

Conductivity _. (pimhos/cm) Temperature (C) 1--0 ". 2A-41 'L4- - A- zq- k 45.2% DO,(mg/L) 0-.0 "

                                                                                                                                      -4:X.

pH (s.u.) q.;*5 .-..,- Conductivity F3 pH (S.U.) Temperature ('C) 1 .". -'-1 0 "1. 't. kA.' 72.% DO (mg/L) 1 -0 Conductivity (ýimhos/cm) 100%Itk DO (rag/L) 10 ,2- "*'* .3 )- Conductivity (ýimhos/cm) :{ 0- -] *** _ _Temperature Alkalinity ('C)* "-.o R L4. k -Lq. pH (S.U.)Page.57:of (mg CaCO3/L) -10"q101 Ini F [ InitialF.nal

                             . Hardness                    96.                                                                _ ,

TR chlorine <0.0 Ol (m__[*) Initial II I II Final I I Init I Final - P Page 57 of 101

EaviroDmental Testing Solutions, Inc. Pape 7 of 7 EnirnenalTstn Souios .n.Pg f Species: Ceriodaphniadubia Client: Seouovah Nuclear Plant - Non-treated Date: \7-0-- I K 7- 4 Day r Concen-. Parameter- - .< CONTROL ___I1- _ 9.0U) 13% DO (mg/L) -C Q.Oý: (4 -1 1A

                         -Conductivity                        ~~y                                                                         ~'

22.6% DCaO3m/L) ,~ f ___ ___ Temperature ('C) 1 I<__

                                                                          )                  __      __      241ý

_L1_ 14I 2A ~ 3 j. 11.3% DO (mg/L) b, 0 Conductivity 01 " ,4 (pl-nhos/cm)Il

                  -Temperature         (T)       ZA-"          _3Ak            2LA              2A-                        ZSO

_PH(S.U.) 6.02 PCC'j 80- ___3__ e_,0 22.6% DO (mg/L) Conductivity Z( (prnhos/cm)________ ____ __ _ _ _ Tempeerature ('C) 2MI _" l-.

                                                                               -LA~            1Ak.'        14.1          TA__k_4__

Pi U. R (S 61 5 .0 b.0 45.0% DO (mg/L) _____Temnperature (T) 1A ' 2LI.( 24.9 -01.0O 'ti- t'-.(R Is PH e-oCl "4( U.)i ID, -7 -UT.13-12) ,2 1006% DO (mg/L) 079 9. .0 -- 8 - Conductivity A Ternperature ('C) 7(4. 4.1 2T.I ?NI.C 2L..1 L'D. 24. 4 I Initial I Final I Initial Final I Initial aInitial a Final ! I Page 58 of 101

Environmental Testing Solutions, Inc. Page. // Page / of Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0. 10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst T I o6 I lodidereagent: JAk/,' '-o Date analyzed /-Z. - Cý/-0 ý Acid reagent: _p . *o Calibration: mg[ 1.00mg/L Referencesta number sl / Z ) CS -, Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L)

                "v               B lank      (should be   <0.10mg/L)       ..   ..     ..   ..       . ......          ....  . ..   ...._._

cTL~.. 10 tilI7(d= WT'T'" ctQu~dlu'*(p1.,4 btc~n o.oootto% 01 ItCY 0 Z o a{ "/V Ul 110A.O-0 *71N.ID~¢ 1 ,71,115 Ust~o-V t~totd [cl t4.iýtkba L-0.000002,J Lb-U 2A kP o Low "o4, L- 0l Cn I-ts 0.OSM A/ S-I a I0LI 31F264 " -LO.OOOM OF-2- 011)AZ0.03-11 IO CoI%O,23. 45&v T',47P *.o.*o 0 ._*n-Q.,3s "k-0).(007O . I~ Cn I rcn I-o'b.o -- 0 V kVcY1 L~C Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratorycontrol standard: Reviewed by I 8 I Date reviewed I "IZ0- " Page 59 of 101

Environmental Testing Solutions, Inc. Page I Page 2 of '- Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Iodide reagent: Date analyzed - -0 Acid reagent: Calibration:

0.10mg/L I 1.00mg/L Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the. chlorine levels of the samples.

Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) Note: sap Blank (should be <0.10 mg/L)s s Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Reviewed by I A Date reviewed 1 17 O- " I Page 60 of 101

Environmental Testing Solutions, Inc. Page ___ Page ____ of Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0. 10 mg/L Meter: Accumet Model AR25 pH/lon Meter Analyst "P'D!) Iodide reagent: i:v* Z.C Date analyzed I2 - 6- C' Acid reagent: /,/Vf: 7 Calibration: I <-K~~<<F,*0.10 mg/L i .00 mg/L Reference standard number ,5 1 ///5500 Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV /TV x-100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)

                     /. 1*,/z                               0.50                                         s,.

Duplicatesample precision: ._. Sample Sample ID Sample characteristics Residual chlorine %RPD {(S - D) /[(S+D)/2]) x 100 number. (-g/L) (acceptable range +/- 10%) Duplicate "D " Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) (should be<<0.10 m ) i ,Blank < C ~

                                                                                                                                                                  '- Z'32~

1, 7' La

                --LbOrt        coto stanxdard:                                                4.

I6 "T_ / 0 <Z j Reference standard number Trevle(TV) og/b) Measured value (MV) (mg/b)

                                                                                                                             %/ RS     MNV / TV x 100 (acceptable range 90 to 110%)11 I

0.50 I S - S'4.Z I

                                                                                                                                /<9 *'2, I

I Reviewed by Z1 I-0 Date reviewed I Page 61 of 101

C/ 0L Environmental Testing Solutions, Inc. Page I___ Page I of 3 Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Iodide reagent: TNrql.0Alo Date analyzed LiimOzpa Acid reagent: j-zrtzX0 Calibration: I:**.,:**!.

                   ,      *.+      :,* :.>:¢                0.10 mg/L                1.00 mg/L Reference standard number                    .Z                         NMs,%.

Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) 0.50 O.bOq ILI Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number _(mg/L)

        .________:_-      _    Blank (should be= <010 rmg/L)                                                                     '    Z-0.00 0*      -10"1 0-7 V-Lng      03                                                                      3y~____________~cOS o-1 -z 6,-o a          0&d % e         SS                         *_kFclJVuWA&.N                       .-                      O.oo, 0     I1 O1~ZO~3/4 S4-rb~i~~                                                                              ".Ol0010 a-1 z.oo0.o8 0         uA SS                                       kc-,No Colovo.oOS o-"1120 .o     U     C-oXt L110N Pl,cU          -. t_                        ,NO c6tV                                        0 .00S3 7 a-1 iLpi,\     I                    VMV'0P                       ct4 C ro-0 *{1+/d<,  lo              9pk tCAN                   0. oa a Lo . o     I NI aol 'z  o&. o \                          1I   Ul                    t , po-i    %         c s,

I"t.OR.G E o n-CA CAC. J to aLj'Y 0

                                                                                                                                     ,o.*O1     -001 time (15 minutes) unless otherwise noted, Note: All samples were analyzed in excess of EPA recommended holding Laboratory control standard:

Reference standard True value (TV) Measured value (MV) % RS MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) Reviewed by zI& SIIZ---- Date reviewed 12 - 0 Page 62 of 101

Environmental Testing Solutions, Inc. Page I Page

of 3 Total Residual Chlorine (Orion Electrode Method, Orion 97-70)

Matrix: Water, MDL = 0.10 mg!L Meter: Accumet Model AR25 pHiIon Meter Analyst Iodide reagent: Date analyzed 13.-Oý - C)-1 Acid reagent: Calibration: 0.10 mg/L 1.00 mg/L Reference standard number Note. For samples with a residual chlorine of >.1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory controlstandard: Reference standard True value (TV) Measured value (MV) % RS = MV!/TV x 100 number (rg/L) (mg/L) (acceptable range =90 to 110%)

           -rsS           "                                  0.50                       o.MSTJ                    1              C 9: I-.       "

Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/_) _______ -_______Blank (should be=< 0.10 mg/L) :' `., 0o7 12xl -I *,rav 4, W v,4rTP crtap. poa-. * -o.ooi:3 oC1 zoR, VAc. V dCR (A/*d-.o d ,O31 cri~~~~~to&Coo t NO Io-c U0" t .aloosd* C-~O0 000b9 az al%z* ZrJ- CA

                           . Zy*-&q;0Ya    i            N,'W'V'P           C~ttax, ne~tt            k-0-N Crlvy- Aik             ipt~                   tr~cý               rAOUA-WW(Vo)Uxide-s.                     -"ý4#rA            ~           witc~
               ,-1Th(1.o      5N A CAt,                                    uoý.L,        *(l.oalc-tes                               0.'1, Note: All samples were analyzed in excess of EPA recommended holding time (5 mutes) unless otherwise noted.

Laboratory controlstandard: Reference standard True value (TV) Measured value (MV) % RS =MV / TV x 100 number (mgfL) (ag/L) (acceptable range = 90 to 110%) Reviewed by Date reviewed Page 63 of 101

En~vironmental Testing Solutions, Inc. Page _ Page of 3 Total Residual Chlorine (Orion ElectrodeMethod, Orion 97-70) Matrix: Water, MDL = 0. 10 mg/L Meter: Accurnet Model AR25 pH/lon Meter Analyst Iodide reagent: Date analyzed Ia i Acid reagent: Calibration:

                         .    .1.00        -            0.10 mg/L                            L*g.

Reference standard number I8 : d Note. For samples with a residual chlorine of > 1.0 rag/L, the calibration range must be adjusted to bracket the chlorine !evels of the samples. Laboratory control standard: [ Reference standard number

                ,t$c~

Bt True value (TV) (rag/L) 0-.50 Measured value (MV) O.*t(pq (mg/L)

                                                                                                                          % RS = MV / TV x 100 (acceptable range = 90 to 110%)

92 5. 1 Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L2 B lank (shouldbe(<.10mg/L) * (mg ji (ae t Th:~::i~ 5CN-4o~L CrfYj-ý Cýo5uA 0"t -OJ _______ 0cU- ?Ie-ltý - a1O2NJ.. tkt lot n -k -i 120,1.-

          *al                                             o.5o            S-:N3 o CA*          t*                                  *. * ¢o.M o~O4 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.

Laboratory controlstandard: [ Reference standard True value (TV) Measured value (MV) %/ RS MV/TV x 100 [number I(mg/L) (mg[L) (acceptable range 90 to 110%) S0.50 0.$I~ Cam01 Reviewed by Date reviewed 17-0 0 Page 64 of 101

PaPege f .. Environmental Testing Solutions, Inc. Page -- L.-of Alkalinity (SM 2320 B) Matrix: Water, MDL = 1.0 mg CaCO3 /L Analyst Time initiated [ , Date analyzed ,,. Titrate samples to Time completed / -b 3 pH = 4.50 S.U. Titrant normality andm ultiplier determination:- pH of Normality Normality (N) of H2SO pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml NalC0 3 x 0.05)/E = (N x 50000)!100 ml sample water reference standard nil ml ml = 0.25/E N x 500 4.5 S.U. number number (E) (acceptable range 0.018,- 0.022)

                               '.c* ,,3,**C 1*0-0                    C     . i0.                                                                                  ,

Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO3/L) (ml) nil nl ml = 90 to 110%)

                     'A                     100            100                   . t         9.            M.2,o0
                                                                                                          /t).*)                                                 7.,O/.

Duplicate sampleprecision: _ Sample Alkalinity %RPD Sample. Sample ID volume Begin End Total Multiplier (rag CaCOi/L) f(S - D) /{(S+D)/2]} x 100

                                                                                                                                            ,(acceptable range =10%)

{ number . (nil) al ml ml I'-,up, '9:c )l$. icate Duplicate(B) (B) / *"T.}- )') 5?.2

                                                                                     .5       "'-         ,..K)        DJ.

D -.- 4_--- .*17 Samn1~ mpnejrpmpnic Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (rng CaCO31L) l,.t46.*i4 ' '-iO q*: -'-" Ii').C [O0 .&,) ~.,7 1; _______ _____ "52-I_____. r55j7 85.6 E.).

                                                                                                     /b/. 9 &,,7 a*e*<.*                       ,4;ý                     1o id~t      '7o/.

I/_________,_-___

___, _____,-__ ____- .:,* . 3

                                                                                                     ;7      .,.          ________             __ __ __ _

i;d*-':- .. ,."-Ldt . /u. 37,11 1'ý.q I"i Reviewed by: Date reviewed: [ -- -" Page 65 of 101

Page TI. Page a - of , Environmental Testing Solutions, Inc. Alkalinity (SM 2320 B) Matrix: Water, MvDL = 1.0 mg CaCO 3/L Analyst L ." Time initiated -----. ,. ,* Date analyzed ! . Titrate samples to Time completed pH = 4.50 S.U.

                                               ~
           -T:~...,..f ,,,-,.-na li/v and mnltinlipr        dptprmination:

pHrf---- -Normality Normality (N) of H2 SOj pH Factor or Multiplier Deionized Titrant check -tal'-i( -- End----.al (5 ml NaICO 3 x 0.05)fE = (N x 50000)/ 100 ml sample water reference standard ml ml ml " = N x 500 7 4.5 S.U. number number (E) (acceptable range = 0.0i8 G022r--- Laboratofy control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV /TV x 100 number (TV) volume Begin End Total Multiplier (rmg CaCOjtL) (acceptable range (mg CaCO3L) (ml) ml ml ml= 90 to 110%70) 1PCS~J100 100 3e p u Duplicate sam le precision: Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCOJL) {(S - D) /[(S+D)/2]) x 100 number __ (ml) ml ml ml (acceptable range +/-10%) Duplicate (B) j .. D Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOJL) (mg CaCO3/L) (ril) ml ml ml

                                                                     -,                  i3,            '-.

Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCOJL) MV = A - B (acceptable range (mg CaCO3/L)' 75 to 125%) u s--. !, Sample measurements: Sample volume Begin End Total Alkalinity. Sample number Sample ID (ml) ml ml mnl Multiplier (mg CaCOJL) Ljqq'U) 5i.S. g /Ot,>~,Z ioo .,n 8.5 .3.5 __ -__._ ,5.5-Is:j/.f) :;) ._S /,:. ,D,'3.o 6.o .e _________ L-1!I Q 3t.-, ! 3- -0 315,

                                                                                                                            /Io I 3..(',L.,.,9-                                                                     *x_             I/a.o-       MAY.,     _____       ,
            <1L7tz O .o      1 (7       L,O ', L5,      /               d -OQ          iI ,O           /     '.-) * .         h:*)         I      .

Lflt2o's. '-1

                              ~'ZC1B                           7                 /'.5
                                                                                                                *..        j~+.                I Reviewed by:                                                 Date reviewed: [                       -21.C1 I Page 66 of 101

Page -::. Environmental Testing Solutions, Inc. Page ffIof1 f Alkalinity (SM 2320 B) Matrix: Water, MDL = 1.0 mng CaCO 3/L Analyst Time initiated Date analyzed iz2 ,G!*' Titrate samples to Time completed pH = 4.50 S.U. Titrant normality and multiplier determination:. pH of Normality Normality (N) of HSO4 pH Factor or Multiplier Deionized Titr i-----elck- Begin End Total = (5 ml Na2 CO 3 x 0.05)/E (N x 50000)/ 100 ml sample water reference standard ml = 0.25/E = N x 500

         = 4.5 S.U. number            number                        (E)     (acc     bte-rpge-=-L0-0  -80.022)

Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L), (acceptable i-ange (nbg CaCO/L) (ml) ml ml ml = 90 to 110%) Duplicate sam le precision:_ _...... Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO3!L) {(S - D) /[(S+D)/2]) x 100 number (ml) ml ml ml (acceptable range t 10%) 1 ;.) ' , IC c(B-! DZOub.O ) ) ") Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOJL) o L Os -. LS , 2- 3.Z 3'4. U-

                             ,.       ~.3                                      Y l)-ij9..           10. e cf1c       O~o 1-                            2n                          oC1
                                                                               .        (9, Ll        -

(!SL t _ _ _ _ _ Page 67 of 101 Reviewed by: w Date reviewed: L e" i

Pace "'/; Environmental Testing Solutions, Inc. Page -i of Alkalinity (SM 2320 B) Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst }J,- Time initiated - Date analyzed Titrate samples to Time completed pH = 4.50 S.U. Titrant normality and mutihplier determination: pH o . . Normality Normality (N) of HSO4 pH Factor or Multiplier water reference standard ml ml ml =0:25/I._ N x 500 4.5 S.U. number number (E) (acceptable range = 0.018- 0.02.5-........J Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % ES = MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJL) (acceptable range (rag CaCO 3/L) (ril) ml ml 90 to 110%) DuLlicate sample precision:. Sample Alkaliriity %RPD

             .Sample.             Sample ED               volume       Begin      End     Total       Multiplier         (mg CaCOJL)             ((S - D) /[(S+D)/2]} x 100 number                                         (ml)       ml         ml        ml    /'3,                 S                      (acceptable range         10%)

Duplicate(B) V. ./(,1 . . D Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L) (mg CaCO 3/L) (ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) '% R = MV / SV x 100 (mg CaCO 3/L) MV = A- B (acceptable range

                                                                ...                    (nmg CaCO3./L)                               =75 to 125%)

340 9O9.7 Sample measurements: SSample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3IL) on i-Zon . I I -.P( t*. &S /- *.. q4_4 3.-*..0 ) /O b-0 q *0,o'So i rcv.h

                                      -*              oo(z               !oe              0.-u .q              .9,,3i,_
                                    .]*-l*,;*         005                 I*                       26,.-o 5,j                              -

o9- 9. 0¢,I* I p./y..,* 21.2. *i..

_ ____..

011 zs J O*--oz~.0,47- 'J,' *) T*/ý_ '*z/0.0 3 .___**- 3.e) 93 7 01a 2.*.7 n 52 - _.0 _o__ on*Zo.oq

                                 <g*-sx) tr                I1I0.                                    e.5 3.-                ,

Z, Reviewed b Y: (4 ILJUDate reviewed: 1 1-2-21" 0".1 Page 68 of 101

P-age 77 Environmental Testing Solutions, Inc. Page ___j Alkalinity (SM 2320 B)

Matrix: Water, ivfDL = 1.0 mg CaCO 3 1L Analyst " LV& Time initiated --. _

Date analyzed t Titrate samples to Time completed pH = 4.50 S.U, Titrant normality and multiplier determination:

          -pH--               --             Normality                                             Normality (N) of H2SO                       pH Factor or Multiplier Deionized           Titrant "-            -----
                                                     ..          egin--
                                                                      .End.         Total         =  (5 nil Na 2 CO 3 x 0.05)IE                (N x 50000)! 100 nil sample water           reference       standard           ml          ml                  .. .l. .... =0.25/E ..                                 =Nx 500
           = 4.5 S.U.          number          number -         _(E)                          (acceptable range 0.018 - 0.022)

Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (rmg CaCO 3/L) (acceptable range (rmg CaCO3/L) () l ml ml= 90 to 110%)

                     ..:S,.-, t-100                                                  '94     (,L               J(. U0                    I                 L       j  i.

Duplicate sam le precision: .. Sample Alkalinity %RPD = Sample Sample ID volume Begin End. Total Multiplier (rag CaCO3fL) {(S - D) /[(S+D)/2]} x 100 number ml ni(ml) "ml ml (acceptable range t- 10%) D~*._ Duplicate (B) / Ds~ _____9 Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) nl ml ml Multiplier (mg CaCO 3 /L) (31 i-Zc)'.* 7/-'sQz,*[3/4) / .5j3 ' -** 43.2 3,, z-* /4.12)

           -t    -zoS.t1-

o. VLk.c-- .4(3Z 3- 3.i cftc & l , . 0 /-5 . .3,,

_ ,__ S. 3 . *zo., i .S .3.cO 0112 _C1 1Z)_

                             .                                                                                      .'3
              ,2-CA-oC-                             __.2___            __       _    _      ~    .z.   [*o-q       ,5.1                               __..           _   _

Page 69 of 101 Reviewed by: m Date reviewed: I t-21-0-1

Page Environmental Testing Solutions, Inc. Page off Alkalinity (SM 2320 B) Matrix: Water, MDL = 1 .0 mg CaCO 3/L Analyst . Time initiated Date analyzed . ", Titrate samples to Time completed . . pH = 4.50 S.U. Titrant normality and multiplier determination: pH of Normality Normality (N) of H 2SO pH Factor or Multiplier Deionized Titrant check Begin End Total (5 mil Na 2 CO 3 x 0.05)/E = (N x 50000)!100 ml sample Ta-t-r- reference sa-n-farr -ff--M---ml------- -- 0.25*E =N x 500

        = 4.5 S.U.          number           number                              (E)       (acceptable range = 0.0T8---02-2)--

Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS MV TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOIL) (acceptable range (mg CaCOjIL) (ml) ml ml rl = 90 to 110%) 4C)Vo 3e,1/2s¢* 2c:1?z 3 ID!* K) C3o/." ! ___ __ ___ 00__ 100 6! Duplicate sample precision: Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mgCaCO3/L) (iS - D) /[(S+D)/2l} x 100 number (ml) ml mln ml (acceptable range t 10%) Duplicate (B) @ , ;" "G *O D ,,40,. "

~ ~

Cri0I IU ~ 0 it0 LA -0 2 --- Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOa/L) (mg CaCO3IL) (ml) ml ml ml 07,-1 ,0. lO!3 M-_3 io -co 0 Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (rg CaCOVIL) MV = A - B (acceptable range (mng CaCO3jL) 75 to 125%) Samle measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mng CaCO3 L) C zita- a4,- C 2 /00 10.3 :.;.o (_3 _ _-_ 071 20-.0 3 153I Ao1.0__

                                                 "--                                                             5-16 n 12'6 00.3q.o                                                                                 ~        1 2 12 05 -_1_

LEI~z8 (0 _7_ __ Reviewed by: Date reviewed: 1l- Z. - 1 Page 70 of 101

            'nvironmental Testing Solutions. Inc.                                                                                             Pie              _    of Total Hardness (SM 2340 C)

Matrix: Water, MDL = 1.0mg CaCO 3/L Analyst Time initiated Date analyzed t i 7:O

                                              . -:,)                                                                          Time completed Titrantnormality and multiplier determination:

Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard mn] ml ml = 0.2fE = (N x 50000)/ 50 nml sample number number (E) (acceptable range- 0.018 - 0.022) N x 1000 Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS = MV! TV x 100 number (TV) volume Begin End Total Multiplier (rug CaCO1 JL) (acceptable range (mg CaCOi/L) (ml) ml ml ml 90 to 110%) 40 50 , -;j, *. j '// Duplicate sam ple precision: .... Sample Hardness %RPD = Sample Sample ID' volume Begin End Total Multiplier (rug CaCOJL) {(S - D) /[(S+D)/2]} x 100 number (ml) ml m ml nd (acceptable range t 10%) Duplicate (B) D Matrix spike recovery: -, Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (rug CaCOy'L) (rug CaCOi/L) (ml) ml ml ml Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (rmg CaCO 3/L) MV = A -B (acceptable range (rug CaCO3 IL) = 75 to 1250o) qq qo,oy/ Sample measurements: Sample volume Begin, End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOg'L) Blank . d ) g,. (should be = 0 mg CaCO3 /L) N.0 I ')-1i *-/ 8~ LflL 4.gL / Id&T)I___3c

4 4, _________

                       .____,                                                I__,_,4t            i*C*;'vI-.         q.             I                            2
                                  ,o.6JuiLo______ i '*,uL                                             g:>.*       g.        ______                         '4/
               >-,-' n7
                                                                                                       *.7- 'K .*0-67 I          *o '-'~                        *..         ,

i*--*,*'* 7** <,-,- f.n0,a, q. ____. ____ ______ ____.o __,,____. t* , J!. __ __ __ Note If >lSml of titrant is used, sample must hediluted. Reviewed by: [D Date reviewed r eZ] Page 71 oflO101-

Pageof P a ge *- of __ __ _ Environmental Testing Solutions, Inc. Total Hardness (SM 2340 C) Matrix: Water, MDL- 1.0 mg CaCO 3 /L Analyst Time initiated Date analyzed / ..- ,-.- Time completed Titrant normality and multiplier determination:

        - Titrant         Normality check                   Begin          End       Total                Normality (N) of EDTA                        pH Factor or Multiplier reference               standard                       ml         nd        ml                            = 0.2EA                            (.V , 50000) 50 n-d sample n n-be     - ........              .....    ............                     '- .        (    ceprabe-rn      -     ,.022)           "N                x 1000 Matrix spike recovery:,

Reference standard Spike value Sample Spike hardness'(A) number (SV) volume Begin End Total Multiplier (nmg CaCO 3/L) (mig CaCO3yt) (1-11) nil nil mlI W*0 S3.,q .- c,.3 '30 Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCOy'L) MV = A - B (acceptable range S(mg CaCOIL) 75 to 125%) 8 '420 Sample measurements.- Sample volume Begin End Total Hardness Sample number Sample ID (nl) ml ml ml Multiplier (OgCaCOJL) (should be = 0 mg CaCO3/L) al 1 0:5 '15 oqa o -. 3/4'~~'zq3*

                                                                      *,                         :* _ .3;2                Jo       ____        ___

01. lu,* .ZS_ i) iv'Y/61; ,r*lo -# 1) rd _ _ _ k,..

5:1 3). I.i o _ _ _ _ _ _ _ an_______ I . . ,A. Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: Date reviewed [ 2-..(1 Page 72 of 101 ,-

Environmentai Testing Solutions. Inc. Pae

of*""

                                                                                    *Total Hardness (SM 2340 C)

Matrix: Water, MDL = 1.0 mg CaCO 3 /l Analyst Time initiated Date analyzed Time completed Normality (N) of EDTA pH Factor or Multiplier

                                                                                                             = 0.2/E                         -  (N x 50000)/ 50 ml sample (acceptable range = 0.018 - 0.022)                      = N x 1000 Matrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (rag CaCOIL) (mng CaCOj/L) (ml) ml ml Sample hardness (B) Measured spike value (MV) % R = MV /SV x 100 (ig CaCOpeL) MV = A-B (acceptable range (rag CaCOy'L) = 75 to 125%) Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (rng CaCO 3/L) Blank *_. U" Irzoi. __-,. 0 _____ Td-*,t.. O (. r 3m

                                                                          -.-----.--.         4),,        .!    .     ,,--1,--o ao..Z* .~.                            ,[. 7/                   4,,            l            t9I*.t       j}**          t,                      m Cn 1Al~  0 0Al                                                                       123       le.i          T      I~~

Note: If >15mI of titrant is used, sample must be diluted. Reviewed by: E :::_ Date reviewed 1 %'2-'I26 I Page 73 of 101 .

_______'+ of

IEnviron3mental Testing Solutions,

[nc. Pac Page :' Total Hardness (SM 2340 C) Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst Time iniiated . I ~Date analyzed Time completed I Titrant normality and m ult lier determinat Titrant referec.e _ Normality check standard Begin m t;on: End rnI Total ml ().. Normality (N) of EDTA

                                                                                                              = 0.2/E range = 0.018 - 0.022)
                                                                                                                                              =

pH Factor or Multiplier (IV. 50000)/ 50 nil sample

                                                                                                                                                         = N x 1000 number .           nUM-b-r       -                                              .(acceptable Laboratory control standard.

Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mig CaCOJL) (acceptable range (nmg CaCO 3/L) (ml) ml ml ml 90 to 110%) I 40 50 Duelicate sam le precision. I Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (rag CaCO31L) {(S - D) /[(S+D)/21) x 100 number (ml). ml ml ml (acceptable range +/- 10%) _ _ _ _s Duplicate. (B) ý4 el n.

                                                                                     ;..,.e,*o*. 4t.,          4"          D ,;                           -. 11 I             IMatrix spike recovery:                 -

Reference standard Spike value Sample number (SV) volume (mg CaCOL) (mnl) Sample hardness C (rmg CaCO 3JL) Sample measurements: Sample volume Begin End Total HIardness ISample number Sample iD (ml) ml ml ml Multiplier (mg CaCOJL) (should be = 0 mg CaCO3/L) I on 0"1 l1 o'j.o3 Q 015- 0.

                                      \, 5,J
                                      -1"2"...2 17Z"OS..-                   ,

50 4, LtII iL ____ ______ crn* Y 0 S. c :,J T'L-3 - I (TI I -Z0*1 ____________ 2.1~ l j.j_ qi- ________

                                                                                                       ? ..q*.tf                        , _1       _        /_

Reviewed by: Dat Date reviewed I k-L- -ZO.- 101If >lSml of titran is used, sample must be diluted. Page 74 ofNotej

Environmental Testing Solutions, Inc. Pa e *' of Total Hardness (SM 2340 C) Matrix: Water, MDL = 1.0 mg CaCO3 /L Analyst1 K', Time initiated Date analyzed K)-,. Time completed . . ant normality and multiplier determination: Normality (N) of EDTA p11 Factor or Multiplier ceptable range = .01-is--)X2"-'- - (N x 50000)/! 0 ml sample

                                                                                                                                         = Nx 1000O Laboratory control standard.'

I Reference standard True value Sample number (TV) volume . Begin (nmg CaCOAL) (Mr) ml

                            , -40                              50 Sample measurements.-

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml nil , ml _Multiplier (Tn CaCO 3/L) (should be 0 mg CaCO3/L)

                           - 6 CnJ       i V                                              4f      -       Ai,           *-tL                8 6"1*,-2. C"J                             -              .-                  a                   j,.-

SPage 75 o t~Oj:If >15ml of titrant is used, sample must be diluted. Reviewed by: Date reviewed,

Sequoyah Nuclear Plant Biomonitoring December 4-1 1, 2007 Appendix D Reference Toxicant Test and Control Chart Page 76 of 101

tnvironmentai i estlng LOiulons, Inc. Pimephalespromelas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water Organism Source: Aquatox, Inc. I.2Z I I I C InI I [oI S I I t USEPA Control Limits (+/- .2 StandardDeviations) 1.0 F-0.8 F-0.6 F-0.4 1.2 t I I I I I I

                                                                                     * . I th I     I
                                                                                                         -th I        I      I    I USEPA Warning and Control Limits (75 and 90 Percentile CVs) 1.0                                        ...........................................

0.8 tt) 0.6 0.4 1.2 j I I I I I I I I I I . I I Laboratory Warning and Control Limits (10th and 25th PercentileCVs) 1.0 0.8 0.6 0.4 I I [I I I I I I I I" I I I I I I Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

        -. Central Tendency (mean IC25)
   ......      Warning Limits (mean IC 25 +/- S A.10 or SA75)
  .......... Control Limits (mean IC25 +/- S 25, SA-90, or 2 Standard Deviations)

Page 77 of 101

a;

 --4 0o                                                                                                      Environmental Testing Solutions, Inc.

Pimephalespromelas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water Organism Source: Aquatox, Inc. State and USEPA Laboratory Laboratory USEI'A USEPA Test number Test date 7-day ICs CT S Control Limits SA.10 Wanting Limits Sý25 Control Limits SA.1s Waning Limits SA9o Control Limits (g/L KCI) (g/LfKCI) CT-2S CT+2S CT - So CT + Skto CT - S,,~ CT + S,,, CT - S,,, CT + S,,, CT - S, 9o CT + Sýgo 02-20-07 0.59 2 03-06-07 0.71 0.65 0.09 0.48 0.83 0.08 0.57 0.73 0.14 0.52 0.79 0.25 0.40 0.90 0.29 0.36 0.95 0.13 3 04-03-07 0.53 0.61 0.09 0.42 0.80 0.07 0.54. 0.68 0.13 0.48 0.74 0.23 0.38 0.84 0.28 0.34 0.89 0.15 4 04-03-07 0.79 0.66 0.12 0.42 0.90 0.08 0.58 0.74 0.14 0.52 0.80 0,25 0.41 0.91 0.30 0.36 0.95 0.18 5 05-08-07 0.64 0.65 0.10 0.45 0.86 0.08 0.58 0.73 0.14 0.52 0.79 0.25 0.41 0.90 0.29 0.36 0.95 0.16

6. 05-30-07 0.62 0.65 0.09 0.46 0.84 0.08 0.57 0.73 0.14 0.51 0.78 .0.25 0.40 0,89 0.29 0.36 0.94 0.14 7 06-05-07 0,77 0.67 0.10 0.47 0.86 0.08 0.59 0.75 0.14 0.53 0.81 0,25 0.41 0.92 0.30 0.37 0.96 0.15 8 07-10-07 0.73 0.67 0.09 0.49 0.86 0.08 0.59 0.75 0.14 0.53 0.81 0,26 0.42 0.93 0.30 0.37 0.98 0.14 9 07-17-07 0.80 0.69 0.10 0.49 0.88 0.08 0.60 0.77 0.14 0.54 0.83 0,26 0.43 0.95 .0.31 0.38 1.00 0.14 10 07-31-07 0.68 0.69 0.09 0.50 0.87 0.08 0.60 0.77 0.14 0.54 0.83 0,26 0.43 0.95 0.31 0.38 0.99 0.13 11 08-07-07 0.67 0.68 0.09 0.51 0.86 0.08 0.60 0.77 0.14 0.54 0.83 0.26 0.42 0.94 0.31 0.38 0.99 0.13 12 08-14-07 0.64 0.68 0.08 0.51 0.85 0.08 0.60 0.76 0.14 . 0.54 0.82 0.26 0.42 0.94 0.31 0.37 0.99 0.12 13 09-11-07 0.61 0.68 0.08

0.51 0.84 0.08 0.59 0.76 0.14 0.53 0.82 0,26 0.42 0.93 0.30 0.37 0.98 0.12 14 10-02-07 0.67 0.68 0.08 0.52 0.83 0.08 0.59 0.76 0.14 0.53 0.82 0.26 0.42 0.93 0.30 0.37 0.98 0.12 15 10-09-07 0.64 0.67 0.08 0.52 0.83 0.08. 0.59 0.75 0.14 0.53 0.81 0,26 0.42 0.93 0.30 0.37 0.98 0.11 16 10-16-07 0.66 0.67 0.07 0.52 0.82 0:08 0.59 0.75 0,14 0.53 0.81 0.26 0.42 0.93 0.30 0.37 0.98 0.11 17 10-30-07 0.60 0.67 0.07 0.52 0.82 0.08 0.59 0.75 0.14 0.53 0.81 0.25 0.41 0.92 0.30 0.37 0.97 0.11 18 11-06-07 0.72 0.67 0.07 0.52 0.82 0.08 0.59 0.75 0.14 0.53 0.81 0.26 0.42 0.93 0.30 0.37 0.97 0.11 19 11-13-07 0.72 0.67 0.07 0.53 0.82 0.08 0.59 0.75 0.14 0.53 0.82 0.26 0.42 0.93 0.30 0.37 0.98 0.11 20 12-04-07 0.52 0.67 0.08 0.51 0.82 0.08 0.59 0.75 0.14 0.53 0.37 0.81 0.25 0.41 0.92 0.30 0.97 0.12 Note: 7-d IC5 s 7-day.25% inhibition concentration. An "estimation of the concentration ofpotassiuni chloride that would cause a 25% reduction in Pirnephales growth for Uie test population.

CT Central tendency (inean IC2_). S = Standard deviation of the IC2 , values. Laboratory Control and Warning Limits Laboratory control atd warning linits were established using the standard deviation of the IC, values corresponding to the 10th and 25th 5 percentile CVs. These ranges are morc stringent than die control and wanting limits recommended by USEPA for the test method and endpoint. Suo = Standard deviation corresponding to the 10h' percentile CV, (S,t = 0.12) Sxs ý Standard deviation corresponding to the 25'h percentile CV. (SAt25= 0.21) USEPA Control atid Warning Linits S175 ý Standard deviation corresponding to tie 7 5 thpercentile CV. (S7t = 0.38) Sx9o = Standard deviation corresponding to the 90t' percentile CV. (S_ o = 0.45) 9 CV = Coefficient of variation of tre IC25 values. USEPA. 2000. Understanding astd Accounting for Method Variability in Whole Effluent Toxicity Applications Under the Natioatl Pollutant Discharge Elinhýiation Program. EPA-833-R-00-003. US EnvironnmentalProtection Agency, Ciocinnati, OH. Organi-ms obtainedfrom Aquatox, Inc. atox12-04707

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Pimephalespromelas / Potassium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Organism Source: Aquatox, Inc. Test number Test date Control Control Mean CT CV CT MSD PMSD CT Survival Growth for Control Growth for Control N) (mg/larvae) (mg/larvae) N Growth CV (%) for PMSD 1 02-20-07 100 0531 6.3 0.12 22.8

2. 03-06-07 100 0.734 0.632 15.6 11.0 0.12 15.8 19.3 3 04-03-07 100 0.724 0.663 14.8 12.2 0.11 15.8 18.1 4 04-03-07 100 0.763 0.688 3.7 10.1 0.12 15.9 17.6 5 05-08-07 100 0.883 0.727 6.1 9.3 0.09 10.5 16.2 6 05-30-07 100 0.790 0.737 12.2 9.8 0.11 13.6 15.7 7 06-05-07 100 0.644 0.724 11.9 10.1 0.11 17.1 15.9 8 07-10-07 100 0.693 0.720 2.3 9.1 0.05 7.5 14.9 9 07-17-07 100 0.685 0.716 8.5 9.0 0.08 12.2 14.6 10 07-31-07 100 0.686 0.713 7.9 8.9 0.08 11.0 14.2 11 08-07-07 100 0.854 0.726 11.5 9.2 0.13 15.7 14.4 12 08-14-07 97.5 0.900 0.740 13.1 9.5 0.13 14.4 14.4 13 09-11-07 100 0.831 0.747 6.3 9.2 0.14 17.4 14.6 14 10-02-07 100 0.918 0.760 4.9 8.9 0.17 18.1 14,8 15 10-09-07 100 0.772 0.760 7.9 8.9 0.08 10.2 14.5 16 10-16-07 100 0.662 0.754 13.6 9.2 0.08 12.8 14.4 17 10-30-07 100 0.771 0.755 12.0 9.3 0.16 20.1 14.8 18 11-06-07 100 0.624 0.748 6.4 9.2 0.09 14.7 14.8 19 11-13-07 100 0.603 0.740 14.2 9.4 0.10 16.8 14.9 20 12-04-07 100 0.756 0.741 7.9 9.4 0.11 14.5 14.8 Note: CV = Coefficient of variation for control growth Lower CV bound determined by USEPA (10'h percentile)= 3.5%.

Upper CV bound determined by USEPA ( 9 0ih percentile) = 20% MSD - Minimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10' percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile) = 30%. CT= Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program, EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. 0 USEPA. 2 01a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtainedfrom Aquatox, Inc. atoxN 2-04-07 Page 79 of 101

tnvironmentai - esnng koiutions, inC. PimnephalespromelasControl Growth, Coefficient of Variation, and PMSD in Potassium Chloride Chronic Reference Toxicant Tests Organism Source:. Aquatox, Inc. 1.25 I I l [ I 1.00 0.75 0.50 0.25 USEPA Acceptance Criteria (> 0.25 mg per surviving larvae) I I I I I t I r .

               -      I      I       I7            I            I       ..I            I                    I                 I              I       I              -I          I   I                       I I 30 Kentucky Acceptance Limit (< 30.0%)

0 0 20 . . .. .. . .. 0 Z 0 10 0 0 I I I .....I. I.I I I I I I.I.. 30 20 10 A kJ z' Q'I z' Test date

   -        Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

Central Tendency (mean Control Growth, CV, or PMSD) Page 80 of 11j1ntrol Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)

Environmental Testing Solutions, Inc. Page I of 5 Environmeidal Testin2 Solutions. Inc. Page 1 of5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimeph ales promelas PpKCICR Test Number: Dilution preparationinformation: Comments: KCI CHM number: C___K______ Stock preparation: 50 g KCI/L: Dissolve 50 g KC( in I-L Deionized water Dilution prep (mg/L) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Test information: Organism age: '2 t - Vbu4S. Atlý Randomizing template: eut~no - se !Ifj Date and times organisms %L - fl1 thpb Incubator number and were born between: shelf location: Organism source: PITh'b. 6A-TCi Pp %-Z-0o*-. Artemia lot number: .'tuq \j Transfer bowl information: pH = SU Temperature = 'C Total drying time: "_-_O__%_

                                                             "1 .'                          "~Lb _       Date/.Time in:                  *
                                                                                                                                         %15-a\-a Average transfer volume:                . ,                                             Date / Time out: .1r.-d1-        t25o L

Oven temperature: IQE" .- Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, MHS Analyst feeding feeding time renewal, or batch used time termination time 0 0-1 ----4 1S - li:< -

                                                                                                                    -z\-cx1  A 2           Q2-016-0"1     o~       o        N4S                       v0\3               x.A-as           C1 3        I-l.- "--a-}      02S               Ilk_______

ZQ.o1-of o 6 11 6 -6 4 oti-+ 1A

                                                                                                    -L          ILi40 Control information:                                                  Acceptance criteria       Summary of test endpoints:
               % Mortality:                                            S7.                  *< 20%             7-day LC 50     - _         , \

Average weight per initial larvae: a.-I(, , NOEC _ _ _ Average weight per surviving larvae: 0. IT S 0.25 mg/larvae LOEC _ _ _ _ ChV IC25 s zo -q I Page 81 of 101

Environmental Testing Solutions, Inc. Page 2 of 5 Environmental Testing Solutions. Inc. Page 2 of 5 Species: Pimephales prom elas PpKCICR Test Number: - 130 Survival and Growth Data Day Control 450 mg KCI/L 600 mg KCI/L A B! C ID E F G IH I J 0 O O o 0 /1(3! 5 O /b /0 0 /C 10 1

                                                                            /Q      /0       /0    /0          /1       /O        C0  /0 2

IQ (0 I l)/(. 0 /0 /0 QO0 t /0 1/ lO 3 J (0 10

                                                                   /0        10              10 10ClO)A.1o       *0       /1 DIc         /)    0      10      10       1t2     1 I0        /0      10       1(3            'I 6                                                                                                         A

____ _ t10 /0/ /0 JO /0C) /0 /0 . q ..-- 7 7-V , t0o 9 /0 t1 10 1U. I* , SA_

                                                                                                                                                 -1 ATrayecolor Pan weight code::(mg) p,     _\%__.14te!I.01    -0 IcaAc Iq.31 13:11 V4,"1q             V"S.S3 ,5.31      13ý.510    *.1,    t4.1   $,
                                                                              .53
                                                                                                                                     ;L Analyst:                  *qI B Pan + Larvae weight (mg) Larv Analyst:    MA4 t7m*a  (mg)qZý,Z,           -L.e I         Ze.igh       1eiZght                           IS-c       1.,      11,oq 1".So C = Larvae weight (mag)
           =A- B Weight per initial number of larvae (mg)                                                                                N
          = C/Initial number of larvae                    1V1                      'p                 0 ID.      0"         01               0"              0*                                          0*

Average Percent -0. , - . - '. weight per reduction initial . from control . number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w wounded. Calculations and data reviewed: Comments: Page82 of 101

Environmen'tal Testing Solutions, Inc. Page 3 of 5 Species: Pimephalespromelas PpKCICR Test Number: 1.A Survival and Growth Data Day 750m KC/L00 KCI/L 1050 mg KCI/L M N 0 P Q R SIT U V X 0 t(3 10 1t5L /( / 5 /I IQ /0 /0 [ao /0 A A 6q -k IA' 44 J4, SS CA' Ci S I -I (D (D 2 S1 A d "2 3 eq & & n -14 to S,

                                                                                                                                     .       4

______ 8` t'%sa*A *, *& , 2.kIa 5 L~ O~ ~s g,

0 A 2.e A = Pan weight (mg)

Analyst: A B =Pan + Larvae weight (mg) ~j).l~ ~ g,4 ~ ~ 1~ i7 ~3-341 Larvae weight (mg) A-B*- 4-i11 3.) i.%.o 2.4 "Z.$1 3.10 *.* v.-S a.2- __ o-t' Weight perm).* initial numberoflra @ ** I%\tt ,0 * *

v (* *V C/=PInitial numberweigh of larvae Average Percent weight per reduction in itial from control -' s%"*c. *... 7.2.O( "- .~I* . a O. 0 dl0-.!*

number of (%) larvae (ma,) Comment codes: c = clear, d = dead, fg fungus, k = killed, m missing, sk = sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w wounded. Calculations and data reviewed: Comments: Page 83 of 10

4Z M Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephalespromelas Organism Source: Aquatox, Inc. Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: PpKCICR #139 Test dates: December 04-11, 2007 Reveiwed by: Cnnntr-ation (mg(L Rtplite Initiol n.-ber of Final nunber of A- an wenightlong) L.oe- weighl (t.&) Woighl I Surivisng Moon .oighl) Coonlinin of varittIon Weight I mber Meannoonivat Mran weightlnibol P-ornt rodutinn ffom KCI) tIr.-e I.r-ae weight (trg) -A -B nomber of 0I.-na (.og) Srviviong nuber of I( tl,, , I . or 0 of la-non (rag) (%) nu erof atoa-no cariation (%) control (o) l1t-Cot(nig) ,Ooolon.) (0/) (tg) 0.... A 10 10 14.69 23.01 8.32 0.832 0.832 Control B 10 9 14.09 21.11 7.02 0 0.775 6.2 0702 97.5 0.756 7.9 Not applicable C 10 10 .15.95 23.09 7.14 0.714 0.714 D 10 10 14.31 22.05 7.74 0.774 0.774 E 10 10 13.71 19.89 6.18 0.618 0.618 450F to0 10 13.74 20.06 6.32 0.632 0.687 1(.8 0.632 100.0 o.687 11.8 9.1 G 10 10 13.53 20.54 7.01 0.701 0.701 H 10 t0 15.31 23.26 7.95 0.795 0.795 I 10 10 13.56 18.50 4.94 0.494 0.494 600 10 9 15.92 19.26 3.34 0.371 0.502 20.3 0.334 87.5 0.433 16.3 42.7 K 10 9 14.33 19.04 4.71 0.523 0.471 F L 10 7 13.47 17.80 4.33 0.619 0.433 M 10 7 14.50 18.67 4.17 0.596. 0.417 150 N 10 0151551832.00500.527 6 13.86 17.33 3.47 0.578 19.6 0.347 02060.0 0.31 .7 26.3 58.0 0 (0 5 15.53 (8.33 2.80 0.560 0.280 P 10 6 12.90 15.14 2.24 0.373 0.224 Q (0 5 .14.43 16.80 2.37 0.474 0.237 R 10 4 14.38 16.08 1.70 0.425 0.462 I 0.170 S 10 3 14.31 15.57 1.26 0.420 0.126 42.5 0.200 31.7 73.6 T 10 5 14.60 17.25 2.65 0.530 1 0.265 1 U (0 1 13.61 13.73 .0.12 0.000 0.012 1058 V 10 0 0,00 0.00 0.00 0.000 0.000 0.0 0.0005.0 0.019 9.7 97.6 W I10 0 0.00 0.00 0.00 0.000 0.000 X 10 1 14.09 14.71 0.62 0.000 0.062 Dunnelt's NISD value: 0.1095 MSD = Minimum Significant Difference PMSD: 14.5 PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the nminimsoum percent difference between the control and treatient that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound detemained by USEPA (10th percentile) 12%. Upper PMSD bound de.ermssned by USEPA (90th percentile) = 30%. Lower and upper PMSD bounds were detenm'ined from the 10th and 90th percentile, respectively, ofPMSD data from EPA's WET lnterlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 2601a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Enviromtoetntal Protection Agency, Cincinnati, OH. Organisms obtainedfrom Aquaaox, Inc. atoxl2-04-07

cr~ Environmental Testing Solutions, Inc. 00 Statistical Analyses 0 Larval Fish Crowth and Survival Test-7 Day Survival

-5 Start Date:     12/4/2007                    Test ID:       PpKCICR                                  Saraple ID:                    REF-RefToxicant 0                       End Date:       12/11/2007                   Lab ID:        ETS-Envir. Testing Sol.                  Samaple Type:                  KCL-Potassiumn chloride Sample Date:                                 Protocol:      FWCHR-EPA-821-R-02-013                   Test Species:                  PP-Pinsephales promelas Conmlnents:

Conc-mng'L 1 2 3 4 D-Control 1.0000 0.9000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 600 1.0000 0.9000 0.9000 0.7000 750 0.7000 0,6000 0.5000 0.6000 900 0.5000 0.4000 0.3000 0.5000 1050 0.1000 0.0000 0.0000 0.1000 Transforna: Arcsin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV% N Suit Critical Resp Number D-Control 0.9750 1.0000 1.3713 1.2490 1.4120 5.942 4 [ 40 450 1.0000 1.0256 1.4120 1.4120 1.4120 0.000 4 20.00 10.00 0 40 600 0.8750 0.8974 1.2253 0.9912 1.4120 14.199 4 13.50 10.00 5 40

                                 *750          0.6000       0.6154          0.8872         0.7854        0,9912        9,469        4                 10.00         10.00                      16           40
                                  *900         0.4250       0.4359          0.7088         0.5796        0.7854       13.871        4                10.00          10.00                      23           40
                                *1050          0.0500.      0.0513          0.2403         0.1588        0.3218       39.161        4                10.00          10.00                      38           40 Auxiliary Tests                                                                                          Statistic                     Critical                  Skew         Kurt Shapiro-Will/s Test indicates norosal distribution (p. > 0.01)                                        0.95269156                       0.884                 -0.5882964   0.98608425 Equality of variance cannot be confirnied Hypothesis Test (1-tail, 0.05)                    NOEC          LOEC            ChV          TU Steels Many-One Rank Test                         600           750        670.820393 Treatments vs D-Control Maximusm Likelihood-Probit Parameter           Value         SE         95% Fiducial Lnimts                          Control -      Chi-Sq       Critical        P-value         Mu        Sigma         Iter Slope            10.5797807   1.55653174       7.52897844 13.630583                        0.025      5.38853779 7.81472778             0.15      2.90749024 0.09451992        5 Intercept        -25.760609   4.53827135       -34.655621 -16.865597 TSCR            0.01416527    0.01444836       -0.0141535 0.04248405 Point              Probits       mg/L         95% Fiducial Limits ECOI                    2.674 487.084184       388.552161 553.955155 EC05                    3.355 564.961442       477.157271 623.413202 EC10                    3.718 611.445976       531.846681 664.596996 EC15                    3.964 644.950886       571.834898 694.406478 EC20                    4.158 672.884107       605.364523 719.506485 EC25                    4.326 697.810537       635.283585 742.239349 EC'o                    4.747 764.792979       714.272365 806.270707 EC50                    5,000 808.146767       762.708183 851.574385 EC60                    5.253 853.958158       810.216562 904.099344 EC75                    5.674 935.929107         885.93285 1009.83303 EC80                    5,842   970.59983      915.339908 1058.10293 EC85                    6.036 1012.63712       949.647481 1118.70394 EC90                    6.282 1068.12572       993.323362 1201.50749 EC95                    6.645 1156.01018        1059.97762 1337.91255 EC99                   7.326 1340.83853        1193.93693     1641.5599 Organisms obtainedfrom Aquatox, Inc.                                                                                                                                                                        atox12-04-07

Environmental Testing Solutions, Inc. ON Statistical Analyses 0 Larval Fish GrowAh and Survival Test-7 Day Gn-owth 0 Start Date: 12/4/2007 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: / Conc-mg/L 1 2 3 4 D-Control 0.8320 0.7020 0.7140 0.7740 450 0.6180 0.6320 0.7010 0.7950 600 0.4940 0.3340 0.4710 0.4330 750 0.4170 0.3470 0.2800 0.2240 900 0.2370 0.1700 0.1260 0.2650 1050 0.0120 0.0000 0.0000 0.0620 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7555 1.0000 0.7555 0.7020 0.8320 7.934 4 0.7555 1.0000 450 0.6865 0.9087 0.6865 0.6180 0.7950 11.788 4 1.374 2.180, 0.1095 0.6865. 0.9087

             *600        0.4330        0.5731         0.4330         0.3340        0.4940         16.312       4            6.422         2.180       0.1095       0.4330         0.5731 750        0.3170        0.4196         0.3170         0.2240        0.4170         26.341       4                                                   0.3170         0.4196 900        0.1995        0.2641         0.1995         0.1260        0.2650         31.661       4                                                   0.1995         0.2641 1050        0.0185        0.0245         0.0185         0.0000        0.0620        159.711       4                                                   0.0185         0.0245 Auxiliary Tests                                                                                         Statistic                Critical                    Skew            Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01)                                          0.96612656                  0.805                  0.13911643      -1.0072421 Bartlett's Test indicates equal variances (p 0.89)                                                    0.23211719               -9.2103405 Hypothesis Test (1-tail, 0.05)                  NOEC          LOEC           ChV             TU          MSDu       MSDp          MSB           MSE         F-Prob            df Dunnett's Test                                   450           600        519.615242                  0.10947132 0.14489916     0.115353     0.00504333    3.OE-04           2, 9 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point mg/L SD 95% CL(Exp) Skew IC05* 246.36 125.47 . 45.16 602.14 0.2837 IC1O 453.88 78.92 113.63 509.09 -0.8982 ICI5 476.23 36.47 293.20 526.96 -2.2311 IC20 498.58 21.00 433.42 550.11 -1.0351 IC25 520.93 17.71 458.63 572.23 -0.2358 IC40 587.99 .-21.77 543.80 670.46 0.6688 IC50 671.44 40.37 548.59 817.13 0.3585

indicates IC estimate less than the lowest concentration Organisms obtainedfrom Aquatox, Inc. atox12-04-07

Environmental Testind Solutions, Inc. P-aoe 4 of5 Fnvironmen~al Testing Solutions, Inc. Paged of5 Species: Pimephalespromelas PpKCICR Test Number: I2 Daily Chemistrv: Day 0 12 _____Analyst V'ý mt Concentration Parameter V CONTROL pH (S..) /f, __ Q:_ I___, _ -. IConductivity DO (mg/L) (ýimhos/cm)

                                                      *l                       .

fV

CD C Alkalinity * .. '-" (mg CaCO 3/L) .i I Hardness .' (mg CaCO 3/L) __,__,____ Temperature (oC) z21 2 ___.__ __.______z .l zqq *. pH (S.U.) -92,,- O.ii qL ') "71. LU____ DO (muL) ' ,lL. hi ,: "1 450 mg KCI/L Conductivity rem (ýimhos/ctn) o iI 0 F _ _ __ _ _ (oC) Temperature "____. _ _______ ______ --____ pH (S.U.) DO (mg/L) __.___ Q.,' [ 1, o9

t ): * }O07" .

600 mg KC/L Conductivity 17. t * . (prmhos/cm) Temperature _ _ _ (oc) "____- _ __ ___ _. _____ ______. ___.__- -____. _ pH (S.U.) -. 42 LUrL ").S5 ". DO (mg/L) 750 mg KCI/L Conductivity Temperature 2"4zq..sN PH (S.UC) __ _ _ ____. __- _,___. __ __ _ _ - _, _ DO (mg/L) D' 900 mg KCI/L Conductivity %-A, (°C)hoscm) 2- No 4 pH DO (s-u) (C g/L) n _

                                                                            ..            ' 9 .

__'_,_ 0._] __ 9s:-.. _ "_ __ - ".9-'______ 1050 mg Conductivity KCI/L (,umhos/cm)' ____,, (AK' ___ T e m p era tu re 2 4 S1.q . S-STOCK Conductivity C0 Initial Final Initial Final Initial Final Page 87 of 101

Environmental Testing, Solutions, Inc. Page 5 of5 Species: Pimephales promelas PpKC1CR Test Number: Day

                               ,3                                             4                              5                          6 Analvst                              'L-                 -
                                                                    . ...          6 1i"                                ; -NA C oncentration    Param eter CONTROL         pH (S.U.)                        to           .                    -t-      a                .qs    e DO(maL)              ..                                                      '4                            4 Conductivity                                             :z*        : j}A  (5C'Y, (4mhos/cm)                                                                     N Alkalinity (mg CaCO3/L)                                 S Hardness (mg CaCO 3/)

Temperature 2A.1 " , - . -iA. . "- (OC) z ' Lkk pH (S.U.) ). %Cj* -. DO (rnag/L) 1.1T6 450 mg KCI/L Conductivity ( mhos/cm) l ..

Temperature (0c) --,1.- -L.k PH (S.U.) ".q -7 ".2o . 3 5.'0 DO (mg/L) (r.

g KCI/L Conductivity 1- 0 Sj(onthos/cm) HDC Temperature 7zq.o "L... -*.._.o .- .2. -q,. (0c) pH (S.U.) 1 4,4, 4 ) .,cc 6ý, "-1. 0 -Go- 7 7Smg KCI/L 75 Conductivity ( mrrhos/cmn) Temperature (oC) -L Zk. -6 "t. -Lq.g - 5 -L'q pH (S.U.) o -,,* -, 7 .. q ".'bO . DO(mg* . -,. 9050 mg KCI!L Conductivity ( Mhos/cmn) o Temperature (0c) z , .',1. T- a DO (ma/L) 1050 mg Conductivity Temperature 7q.] zq Z, -L.12. 1-k 7L 0 STOCK Conductivity - ,> (pimhos/cm) ,-- . Initial Final Initial Final Initial Final Initial Final 1Page 88 of 101

EjivjiUJIIIIIILIIL~aI XU3LIIIg .. YUIuJLIuII, Li9. Ceriodaphniadubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water i i i i 1.12 USEPA ControlLimits (+/- 2 StandardDeviations) 1.10

         .1.08 1.06 1.04 1.02
                              .-       -           *1       1     1        1        1        1       1     1        1   I       j     I I   III                  I 2.5            I        I        I.      I       I     I        I        I        I       I     I        I           I     I USEPA Warning and Control Limits (75th and 90th Percentile CVs) 2.0 H 1.5 I-1.0 H 0.5 I         I                    I     *I                   I       I     I         I          I    I      I            I      I    r           2 1 A
             .9 I                 I                                                     I      'I I       I        I                 I    1I       I                I           I Laboratory Warning and ControlLimits (101h and 25th Percentile CVs) 1.3 1.2 1.1 1.0 0.9 -

0.8 Q$- is.1 ýabr0 CA-0 O'Cal. 01isl AN JAI 69 Z110 Ný.O -zbNVO N ýA ";,Ob4A Q1.061Q", 6,Y03, QIN- 0ý- 03, QýAZ OQll I 10 )IN IýN ýV Test date 7-day IC2 5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population. Central Tendency (mean IC 25 ) Warning Limits (mean IC25 +/- S A10 or SA.75) aontrol Limits (mean IC25 + SA.2, S or 2 Standard Deviations) pag~e 89 of [1 2 .5 .

0 0 Environmental Testing Solutions, Inc. 0 Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water State and USEPA Laboratory Laboratory USEPA USEPA Test nunsher Test date 7-day IC 25 CT S Control Limits SAAO Warning Linfts S,5s Control Limits SkS Warning Limits Sk.9o Control Linits CV (g/L NaCI) (g/L NaCl) CT-2S CT+2S CT - SN1o CT + SAJo CT - S* 5 s CT + S125 CT - SA.7s CT + S,,T CT - SA,9o CT + Sss 06-06-06 1.06 2 07-11-06 1.09 1.07 0.02 1.03 1.11 0.09 0.98 1.16 0.18 1.25 0.48 0.59 0.89 1.55 0.66 0.41 1.73 0.02 3 08-08-06 1.07 1.07 0.02 " 1.04 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 4 09-12-06 1.06 1.07 0.01 1.04 1.10 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 5 10-03-06 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 6 11-07-06 0.01 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25. 0.48 0.59 1.55 0.66 0.41 1.73 0.01 7 12-12-06 1.09 1.07 0.01 1.04 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 8 01-09-07 1.07 1.07 0.01 1.04 1.10 0.09 0.9"9 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 9 02-06-07 1.09 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 10 03-06-07 1.09 1.74 0.01 1.07 0.01 1.05 1'0 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.01 11 04-03-07 1,10 1,08 . 0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 12 05-08-07 1.74 0.01 1,10 1,08 0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.90 1.26 0.49. 0.59 1.56 0.67 0.41 1.75 0.01 13 06-05-07 1,10 1,08 0.02 1.05 1.11 0.09 1.17 0.99. 0.18 0.90 1.26 '0.49 0.59 1.57 0.67 0.41 14 07-10-07 1.75 0.01 1,06 1,08 0.02 1.05 1.11 . 0.09 0.99 1.16 0.18 0.89 1.26 0.49 0.59 1.56 0.67 0.41 1.75 0.02 15 08-07-07 1,10 1,08 0.02 -1.05 1.11 0.09 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 16 09-05-07 1.08 0.02 1.08 0.02 1.05 1.11 0.09 0.99 1.17 0.18 0.90 1.26 0.49 0.59 1.57 0.67 0.41 1.75 . 0.01 17 10-02-07 1.06 1,08 0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.90 1.26 0.49 0.59 1.56 0.67 0.41 18 11-06-07 1.06 1.75 0.02 1.08 0.02 1.04 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.75 0.02 19 11-06-07 1.07 1,08 0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 26 12-04-07. 1.75 0,01 1.07 1,08 0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.89 1.26' 0.48 0.59 1.56 0.67 0.41 1.74 0.01 Note: 7-d ICzs= 7-day 25% inhibition concentration. An.estiniation of the concentration of sodium chloride CT = Central tendency (niean IC25)." that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. S = Standard deviation of the IC25 values. Laboratory Control and Warning Limits Laboratory control and warning limits were established usiing the standard deviation of the IC 5 values corresponding 2 to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning linits recommended by USEPA for the test nmethod and enidpoint. S,.10 = Standard deviation corresponding to the 10 h percentile CV. (S~js = 0.08) SA.15 = Standard deviation corresponding to the 25t' percentile CV, (SAs5 = 0.17) USEPA Control and Warning Limits SA7s ý Standard deviation corresponding to the 75." percentile CV. (SA7s = 0.45) SA.90 = Standard deviation corresponding to the 90*' percentile CV. (SA0s = 0.62) CV Coefficient of variation of the IC 2 5 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elinsinition Program. EPA-833-R-00-003. US Enviroinetal Protection Agency, Cincimnati, OH. 12-04-07

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Ceriodaphniadubia Sodium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Test Control Control Mean Test date CT CV CT MSD PMSD CT number Survival Reproduction tbr Control Mean for Control (%) (off'spring/female) Reproduction (%). Reproduction (%) for PMSD (%) (offspring/female) CV (%) 1 06-06-06 100 30.3 5.2 3.0 9.8 2 07-11-06 100 29.0 29.7 5.4 5.3 2.5 8.6 9.2 3 08-08-06 100 28.6 29.3 8.9 6.5 3.7 12.8 10.4 4 09-12-06 100 30.9 29.7 5.4 6.2 3.3 10.6 10.5 5 10-03-06 100 32.3 30.2 4.6 5.9 3.1 9.5 10.3 6 11-07-06 100 31.0 30.4 6.3 6.0 2.4 7.8 9.9 7 12-12-06 100 28.0 30.0 6.7 6.1 .2.2 8.0 9.6 8 01-09-07 100 27.6 29.7 4.9 5.9 2.4 8.6 9.5 9 02-06-07 100 29.9 29.7 7.6 6.1 2.9 9.8 9.5 10 03-06-07 100 30.1 29.8 6.0 6.1 2.6 8.7 9.4 11 04-03-07 100 33.1 30.1 4.4 5.9 2.2 6.6 9.2 12 05-08-07 100 32.0 30.2 8.5 6.2 2.7 8.4 9.1 13 06-05-07. 100 28.4 30.1 7.5 6.3 2.5 8.7 9.1 14 07-10-07 1.00 29.3 30.0 6.0 6.2 2.5 8.5 9.0 15 08-07-07 100 .28.7 29.9 7.5 6.3 2.9 10.0 9.1 16 09-05-07 100 29.7 29.9 5.7 6.3 2.5 8.4 9.1 17 10-02-07 100 29.5 29.9 .9.4 6.5 2.4 8.2 9.0 18 11-06-07 100 28.0 29.8 6.1 6.4 2.5 8.8 9.0 19 11-06-07 100 30.4 29.8 5.6 6.4 2.2 7.2 8.9 20 12-04-07 100 31.8 29.9 5.5 6.4 2.5 7.9 8.8 Note: CV= Coefficient' of variation for control reproduction. Lower. CV bound determined by USEPA ( 1 0 th percentile) = 8.9%. Upper CV bound determined by USEPA (9 0 th percentile) = 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment That can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA ( 1 0 th percentile) = 13%. Upper PMSD bound determined by USEPA ( 9 0 th percentile) = 47%. CT Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-82 I-B-01-004 and EPA-821-B-0.1-005. US Environmental Protection Agency, Cincinnati, OH. 12-04-07 Page 91 of 101

Environmental r esting soiutlons, inc. Ceriodaphniadubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests 40 I I I ... . .. .. . .. .. . .. . .. . . .. . . .. . . ... 0 __ 35 30 o ~ i~ 25 o

      ~      20 o

Q 15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female) S I I I I I I I I I I I-t*

I -

                      -   I I I            I     !   I         I    1. 1           I    I 1     1                I       I      I   I          I      -
  '-,     0  40 North Carolina Acceptance Limit (< 40.0%)

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00 QC~. i~~. f i I ! .... I ! I........... 50 I I I I I I _ I I I I I I I I I USEPA Upper PMSD Bound ( 9 0 "hpercentile < 47,0%) 40 30 20 10 I .. .I I .i i

                                             . .I         I      I      I               ........... I             I n3 U/

Test date

  -            Control Reproduction, Coefficient of.Variation (CV), or Percent Minimum-Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
       -    *Central Tendency (mean Control Reproduction, CV, or PMSD)

Page 92 of lAJlntrol Limits (mean Control Reproduction, CV, or PMSD + 2 Standard Deviations)

Environmental Testing Solutions, Inc. Page 1 of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphniadubia CdNaCLCR 4: tOk Dilution preparationinformation: Comments: NaC! CHM number: - (___.4 ____"_-,_ Stock preparation: 100 g NaCkIl (dissolve 50 g NaCI in 500 ml deionized water) Dilution prep (mg(L) 600 800 1000 1200 1400 Stock volume (miL) 9 12 115 I8 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500. Test organismsource information: Test information: Organism age: -< 24-hours old Randomizing template color: 'eVR-A-e. Date and times organisms were born I-..O"-r 0 t-L T-ro ItW' Incubator number and shelf between: location: Culture board:. i\- '.fVO" __ Replicate number: I 2 3 1 4 5 6 7 8 YWT.batch: to-i. al Culture board cu number: 1. 1 ' Ito Lt 'Z Iq 17 Transfer bowl information: pH =-. " SU Temperature= A. 9C Selenastrum batch: .\ -1

                                                                                                                                 -J Daily renewal information:

Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0A 2 1L-ai-cr I t-i-at-U) 026 4 1-(--0\ I-O-t-fq.-l 5 . -- s ,.o4-01 Control information: Acceptance criteria Summary of test endpoints:

            % of Male Adults:                                    t*7,                               :5 20%               7-day LCso            'qoco
            % Adults having 3rdBroods:                            C01.            -                  - 80%               NOEC                   icOo M
            % Mortality:                                         07.                                *20%                 LOEC                    1.0 Mean Offspring/Female:                               \                          15.0 offspring/female        ChV                    l(%A.,
            %CV:                                              .2                                  < 40.0 %             I IC25                    11.2.

Page 93 of 101

Environmental Testing Solutions, Inc. Page 2 of 6 Environmental Testing Solutions, Inc. ?aoe2 of6 Species: Ceriodaphniadubia CdNaCLCR #: ___ I CONTROL Survival and Reproduction Data Replicate number Da 2 3 4 5 6 7 8 9 10 I Young prodced _) Adult mortality I '7 Yourspoue ___ q1 c) ~fS t 3 Young produced C CA C) C ) Adult mortality j L .i --.. '*'-[ ' L._ produced J mortality jioug Adult ~ 1' ]3~__ - "J\...._ N 5 [ Au morta Young produced l A J__~-~k 0] 0 Iz 10 1_2-Lol1 6 Adlmralt Young produced 0 J -d--I' 7 [ Young produced 11,o 1k \ko

                                                                                 %to                                        ', -A        L.               <                   k       ,

Total young produced 7_ _5 2 3 3 2.9 t z_ Final Adult Mortality ... ...- * " .. ... _ ._ . ',.. '- . ... X for 3rd Broods K( 1- X- ,< j . > Note. Adult mortality (L = live, D =dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over w ith adultrduring transfer)- _ _Co n c e ntr ati o n : Concentration:

                                                                                                                        % Mortality:                                                  -

N Mean OffspringFemale: 31, V" 600 mg NaCUL Survival and Reproduction Data N Replicate number Da _ 1 2 3 4 5 6 7 8 9 10 N 1 Young produced Adult mortality Young producedLQ

                                                           .          U I

o-IJJ .I't. U O c) V 0 l* l 0 2

                          .Adult mortality      J               1                                                  .___I j--...                           U t--I ....... _

N 3 Adult mortality

                        *~Young     produced 1               ILC)~~~~~

c) .L) II .. T .T c

                                                                                                                                                         -I
                                                                                                                                                                            -71 4[Young      produced    J      4         LA~                            il$                      __          __                S-         L L[ A N ae94o                       Adult mortality                        *              '--       II-[                                                     I _I                           --

igproduced (J 3 0~7jQ C) 2 ~ ~ 7 -Youngproduced l ' IS t to ILI iI q 1- 1i .-I 'S Total young produced :53S 3,z. 31 3" '2. 3Z 31A Final Adult Mortality I 2 -- k. . i _. - I L .. te: I.uult morality .L- U - uau), ) -b spi Uitbood 51igie ood Spuit ueLtven tWO Udays), 0L carry ovetou (Olsprlng ca, Fried over with adult during transfer). Concentration:

                                                                                                                       % Mortality: -                                            W.

Mean Offspring/Female: 3Z,8

          .01
                                                                                                                       % Reduction from Control:                               -3. k        I',

Environmental Testing Solutions, Inc. Page 3 of 6 Species: Ceriodaphniadubia CdNaCLCR #: 1\ 800 mp NaCIIL Survival and Reproduction Data " Replicate number Dav 1 2 3 4 5 6 7 8 9 10 I Ydung produced " 0 C Q. 0 j , _ n 2 Young prdue Adult mortality . . 31 Young produced C) O ~ r)L ___ __ A dult m ortality 4 Young produced Adult mortality "___ -_ 5 I Young produced Adult mo tlt I'I' 1a -- I t'].~1 I~l]~ k._Ik - k..._-II Young produced o 01 Adult t _ortal_-ty 74 .$ Young produced

               'Total young produced Final Adult Mfortality J             31
                                                                   \-

1. 3 C K 3' 30 1

                                                                                                                                      .3%A 1
                                                                                                                                                    -6q 21~ 3 1 -I I,..

1 Note." Adult mortality (L = live, D = dead), SB =split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer). Concentration:

                                                                                                                  % Mortality:                                        67.

Mean Offspring/Female: 3\ A

                                                                                                                  % Reduction from Control:                        - 0.37.

1000 mg NaCIfL Survival and Reproduction Data Replicate number Day 1 .Young produced Adult mortality 1 J_ 2 C) 3 L ._ I_ .__ I* 0_ 4 0 5 0 6 7 8 o0. 9 K 10 cQ rT 2 Youngproduced ° l0°,°'° - °, Adult mortality I L- £ " _ - -L-IL- I I 3 Adult mortality Young produced ' c- *_ [ L"] r* I ,,._ ' -I 4 Young produced -5 s 4[-I T L .3 ALFq Adult mortality __ 1 .1 II _ .. I,..t... .. jL-5 Young produced Ij. J ) 3T LIf i[ iI{ vzjv2.. Adult mortality I ._ I - L *J*tL j _ 6 Young produced rQ Q ) CQ 0 0 V Io 7C _

                             .Adult mortality                                      ,    -.-       I                I-         -..                             L-             L-I.2          I 7         Young produced                   J"!..           LA IZIA13 Jr,,                             t             l1o                     13            1Z Total young produced                  "'.vt'    --           3\          *              "*              Z             3,           2"1 2             -*     2-8 Final Adult Mortality                       _    k.          -C,                              .-           -                ..         C...-                       ..-

Note. Adult mortality (L = live, D = dead), 31 - spi utoo' tsin e -'o split between two days), CO = carry over (offspring carried over with adult during transfer). Concentration:

                                                                                                                    % Mortality:                                     2A.j07.

Mean Offspring/Female: a Page 95 of 101 % Reduction from Control: (d.7.

Environmental Testing Solutions, Inc. Page '4 of,6 Species: Ceriodaphniadubia CdNaCLCR #: icL 1200 mgo NaCIfL Survival and Reproduction Data _Replicate number Day 2 3 5 16 71 8 9 10 I Young producd __1 __ _ )0.C L Adult mortality L L_....I '. 2 Young produced Adult mortality 1c1 j---_*-- I

                                                                                                                               ~         I           I         1           I c)[cJc fs-4 I..{

Young produced -n1_O16 ul otaiy Adult mortaliyj. Adult mortality 1 _ __ i t'I L'1

                                                                                                                                                '.-                      k_

5 Young produced 5 _ -A_'A 7 ] IYoung produced - 11 1 (0 Total young produced ia 2-1 l' 12." e s- 'N i$ 1, l3 Final Adult Mortality L . h . - _. 17]...h _ t. ... __. Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring

             .carried over with adult during transfer).

Concentration:

                                                                                                                         % Mortality:                                        OT7.

Mean Offspring/Female: 1. 'A

                                                                                                                         % Reduction from Control:                        5S4,11.

1400 mg NaCLfL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 to I produced Young 0 0 0 C 0 0 0C0 2 Adult mort~ality _ungprduced _ r_

                                                                                                                                                  -  FE     _

I._ 2 Adult mortality. Young produced 10 ]oL c LjC)~~U C Adult mortality 13 Young producejd ] ___ OLA .~ [_ L 4 Young produced ___ . ___ _______ _,__. C._-__ Adult mortality 6 [ Young produced 1 C) 0 Adult mortality : .. . . C-7 [ Young produced ," -7 Total young produced 2'.zo o I... __ 10 Final Adult M ortality xr_._ a J... . -/ -t., 'r-,- A o*A\C * , I e~~l'r

                                                                                  -    A ('/ ;*

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tvore: .Adultmortality kL= live, D'-e - -- Su "t uie brood spit obeween two fays), Cu = carry over (otrspring carried over with adult during transfer). I Concentration:

                                                                                                                          % Mortality:                                T-07.

Mean Offspring/Female: 9-!S

                                                                                                                          % Reduction from Control:                        -70. I         1 Page 96 of 101

Environmental Testing Solutions, Inc. 0 Verification of Ceriodaphnia Reproduction Totals 0 Control 1000rmg NaCIIL Day Re licate number Total Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 3 4 4 4 4 4 4 40 4 5 5 4 5 4 4 4 4 3 4 42 5 11 14 13 13 12 14 10 12 11 11 121 5 10 10 13 12 11 11 13 10 12 12 114 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 16 16 14 17 14 .15 18 16 15 157 7 14 17 14 13 16 14 16 13 .13 13 143 Total 32 34 33 30 33 32 29 34 31 30 318 ,Total 29 32 31 30 31 29 33 27 28 29 299 600 mg NaCIIL 1200 mg NaCI/L Replicate number Total Day Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 5 4 4 5 4 4 43 4 4 5 3 3 3 4 4 4 2 4 36 5 13 14 14 13 11 13 11 12 12 11 124 5 4 7 6 2 1 2 2 2 3 3 32 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 19 15 16 18 14 14 17 15 I8 15 161 7 10 9 6 7 5 8 8 9 8 6 76 Total 36 33 35 35 30 31 32 32 34 30 328 Total 18 21 15 12 9 14 14 15 13 13 144 800 mg NaCl/L 1400 mg NaCI/L D Re licate number Total Re licate number Total Day 1 2 3 4 5 6 7 8 9 10 Day 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 4 4 5 5 4 4 4 43 4 4 4 2 4 3 3 1 2 4 2 29 5 11 11 12 12 13 11 12 12 10 13 117 5 3 4 .2 2 4 1 0 3 3 1 23 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 16 15 15 18 14 17 18 15 14 159 7 6 2 5 5 4 6 *0 3 5 7 43 Total 33 31 31 31 35 30 34 34 29 31 319 Total 13 10 9 11 11 10 1 8 12 10 95

cr~ 0

-t 0

Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphniadubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #69 Test dates: December 04-11, 2007 Reveiwed by: Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (mg/L NaCI) 1 2 3 4 5 6 7 8 9 10 (0/,) (offspring/female) variation (%) control (1!.) Control 32 34 33 30 33 32 29 34 31 30 100 31.8 5.5 Not applicable 600 36 33 35 35 30 31 32 32 34 30 100 32.8 6.6 -3.1 800 33 31 31 31 35 30 34 34 29 31 100 31.9 6.2 -0.3 1000 29 32 31 30 31 29 33 27 28 29 100 29.9 6.2 6.0 1200 18 21 15 12 9. 14 14 .15 13 13 100 14.4 22.7 54.7 1400 13 10 9 II 11 10 1 8 12 10 100 9.5 34.8 70.1 Dunnett's MSD value: 2.528 MSD = Minimum Significant Difference PMSD: 7.9 PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10 th percentile) 13%. Upper PMSD bound determined by USEPA (901h percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 2001 a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-0l-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

-V. Environmental Testing Solutions, Inc. C Statistical Analyses 0 Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 12/4/2007 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mg/L 1 2 3 4 5 6 7' 8- 9 10 D-Control 32.000 34.000 33.000 30.000 33.000 32.000 29.000 34.000 31.000 30.000 600 36.000 33.000 35.000 35.000 30.000 31.000 32.000 32.000 34.000 30.000 800 33.000 31.000 31.000 31.000 35.000 30.000 34.000 34.000 29.000 31.000 1000 29.000 32.000 31.000 30.000 31.000 29.000 33.000 27.000 28.000 29.000 1200 18.000 21.000 15.000 12.000 9.000 14.000 14.000 15.000 13.000 13.000 1400 13.000 10,000 9.000 11.000 11.000 10,000 1.000 8.000 12.000 10.000 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical' MSD Mean N-Mean D-Control 31.800 1.0000 31.800 29.000 34,000 5.507 10 32.300 1.0000 600 32.800 1.0314 32.800 30.000 36.000 6.555 10 -0.905 2.287 2,528 32.300 1.0000 800 31.900 1.0031 31.900 29.000 35.000 6.173 10 -0.090 2.287 2.528 31,900 0.9876 1000 29.900 0.9403 29.900 27.000 33.000 6.197 10 1.719 2.287 2.528 29.900 0.9257

          *1200           14.400        0.4528         14.400        9.000         21.000        22.728       10             15.741           2.287       2.528       14.400          0.4458
          *1400            9.500        0.2987          9.500         1.000        13.000        34.824       10             20.174           2.287       2.528        9.500          0.2941 Auxiliary Tests                                                                                         Statistic                     Critical                   Skew           Kurt Kolmogorov D Test indicates normal distribution (p > 0.01)                                            0.66751724                       1.035                 -0.4971278     2.37645227 Bartlett's Test indicates equal variances (p = 0.19)                                                  7.37160444                   15.0862722 Hypothesis Test (1-tail, 0.05)                   NOEC         LOEC           ChV             TU          MSDu          MSDp            MSB          MSE         F-Prob            df Dunnett's Test                                    1000         1200       1095.44512                  2.52762202    0.07948497      '1062.59     6.10925926   '5.3E-32          5, 54 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point mg/L SD 95% CL Skew IC05 921.5 60.3770384 781.022523 1006.55043 -0.4592 IC10 1010.70968 13.9162817 967.482909 1025.43088 -2.1844 IC 15 1031.54839 7.70863446 1015.09719 1045.89372 -0.3276 IC20 1052.3871 7.3164753 1037.01044 1065.80062 -0.2733 IC25 1073.22581 7.32317592 1058.07312 1086.73913 -0,1340 IC40 1135.74194 9.47972383 1118.60057 1155.0747 0,3823 IC50 1177.41935 12.637724 1158,20347 1208.08873 0.8224 12-04-07

Environmental Testing Solutions, Inc. Paut5,of 6 Species: Ceriodaphniadubia CdNaCLCR #: A Dail I-h . fri Y i~ay 0 I 2 0 Analist V_lkjRi Concentration Parameter pH (S.u.) DO (mo L) CONTROL Conductivity (pmhos/cm), Alkalinity (mg CaCO 3 /L) Hardness (mg CaCO3 /L) __ _ _ _ _ _ (°C ) Temperature _ _ _ ._"__ _, ___ ___ __ _-LA__ _ _ _ .-_ _ _ __ _ __ pH (S.U.) 77.. I 1 DO (moL) 4- 5 600 mg NaCJIL Conductivity " 1 " (+/-mhos/cm) ...... Temperature 7 L 7_q (0c) _ -__ __ -__L___"-__ PH (S.U,) " (41(P. __. __ "____ 14-__

                                                                                                                             .'-        1.-41 DO (mg/L)              *4..

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                                                                                                            .,0                       2.q vH (S.U.)                                             ~%         I iL~

I'~).'5 [~Ji I !9C DO (mg/L) 1400 mg NaCI/L Conductivity

                                                       ,I   I.;_                        'u:y9               L             9 (pmhos/cm)

Temperature (0C) qnz.ti Fina -n t a Fin I nit,al F aq.l STOCK Conductivity (pmhos/cm) Iniial "[Final Initial Final :initial Fia Page 100 of 101

mental Testing Solutions, Inc. Page 5 of 6 mental Testing Solutions, Inc. Enxiron Page 6 of 6 Species: Ceriodaphniadubia CdNaCLCR #: __,90 Dav 3 4 5 6 Analyst rLKVL~ Concen t ra t ion Parameter *0 . ý," 3 1 ' pH (S.U.)H(.U).,~&Ct,; 0,6t 6"a f3. I2, IC e.

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DO (mag/L) q CONTROL *Conductivity (ýmhos/cm) C" . .", Alkalinity, (Mg CaCO 3/L) Hardness (mg CaCO3/L) ., Temperature . (-C) "ZA -LA. ".q PH(S.J.) 1-It. 17 4(ee0 DO (mg/L) 6 600 mg NaCI/L Conductivity i I (pmhos/cm) Temperature I.! toq.- (0C) PH (S.U.) '35 - .0i i.T a.Q. DO(mcL) . "j. 800 mg NaCI/L Conductivity (Imhos/cm)O 1-6 eo Temperature(2q.1 " ,." "L4-( t'.(, . (-C) DO (mgL) p-)Z4* 1000 mg NaC.IL PH (S.U.). Conductivity (ýmhos/cm)

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                     ~~DO (mgL)

Conductivity cs5 ýL 0 i-( rn-hos/cm) 770 Temperature 24.1S Z4j z.9. 1q11 2.4: STOCK ~Conductivity - 41 - -~"- (ýirrhosfcrn) Initial FiaMnFinal inl Iiil Final Initial Final Page 101 of 101

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form/ Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB NNo.2040-0004. (SUBRl 0M1) Address P.O. BOX 2000 (INTEROFFICE SB-2A) TN0026450 103 G F - FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Facilityv TA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD - EFFLUENT YEAR MO DAY YEAR I MOI DAY NO DISCHARGE ATTN: Stephanie A. Howard From 07. 12 01 To 1 07 12 31 NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. !FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS I ~*l. I I- I- F I- r I 1 - PH SAMPLE 7.5 8.7 0 13/31 GRAB MEASUREMENT 12 00400 1 0 0 PERMIT, 60.0 * ***** * *

                                                                                                                                                                                              ý9.0               SU                   THREE!             GRAB REQUIREMENT EFFLUENT GROSS VALUE                                                                                                                         MINIMUM                                      MAXI MUM                                       WEEK F      F                                     F                                                          F        T             .

SOLIDS, TOTAL SUSPENDED SAMPLE 53 73 6 7 0 4/31 GRAB MEASUREMENT 26 19 00530 1 0 0 RPEQRMINT

                                             *"REQUIREM*ENT                                             1250 ,              LBS/DY                                       30               D    L00M,           MG/L                  WEEKLY           "GRAB; EFFLUENT GROSS VALUE                                                      MO AVG____________         -DAILY MX'                                                    MO        AVG.                                                   I                      -   -

I.- . ". _________________

                                                                                                  .                     -FI_________                        I_________                                                    _-4_

OIL AND GREASE SAMPLE <63 4/31 GRAB

                                                                              <51                                              26                                         <6                    <6               19          0 MEASUREMENT 00556     1    0     0                            PERMI'                       190                        250                LBS/.DY                                       1'5                   20 .            MGIL                 WEEKLY              GRAB REQUIREMENT EFFLUENT GROSS VALUE                                                      MO AVG                      DAILY MX~                                                    MO AVG                  DAILY MX

_______ F F F I- F I-~ FLOW, IN CONDUIT OR THRU SAMPLE 1.056 1.356 03 0 31/31 TOTALZ TREATMENT PLANT MEASUREMENT 50050 1 0 0 MGD *

SEITOTAL RýEQU IREMENT.

REPORT~ REPORT.' 4, EFFLUENT GROSS VALUE PERMITI SAMPLE MEASUREMENT SPEAMPIT1 REQUIREMENT SAMPLE PERMI'T'F'7 MEASUREMENT - -FK ~ . ' " REQUIREME$JIETFF NAMrL PICIPLEXEOTVEOFFCE ICeriyunder pnaltofahtti ocumntandal acmnt eore reardundermy direction or supervision in accordance with a system designed to assure that qualified personnel, TELPON DATE Timothy P. Cleary properly gather and evaluate the information submitted. Based on my, inquiry of the person or . persons who manage the system, or those persons directly responsible for gathering the . Principal Environmental EngineerI

                                 *information,                 the information submitted is , to the best of my knowledge and belief, true,                                                                423       843-6700             08      !01          14 Site Vice President                   accurate, and complete. I am aware that there are significant penalties for submitting false         SIGNATURE OF PRINCIPAL EXECUTIVEI information, including the possibility of fine and imprisonment for knowing violations.                 OFFICER OR AUTHORIZED AGENT                     AREA        NUMBER            YEAR         MID    DAYý TYPED OR PRINTED                                                                                                                                                                              CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS                   (Reference all attachments here)

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 TN0026450 107 G F - FINAL _-INTEROFFICE SB-2A) SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER METAL CLEANING WASTE POND Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT I YEAR I MO I DAY I YEAR M DAY

NO DISCHARGE ATTN: Stephanie A. Howard From i07 12 01 Tol 07 i , i , is NOTE: Read instructions before completingl this form.

PARAMETER I .-- i QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE, MAXIMUM UNITS PH SAMPLE ** ** ** ** MEASUREMENT 12 I. .. -I.. 00400 1 0 0 PERMIT " 6.0 9.0 SU . I .. DA RB . EFFLUENT GROSS VALUE

                                                                   +..................I
                                                                                                                     -        I I

MINIMUM1 MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 19 00530 1 0 0" PERMIT ******** 'II .': MG/L DAILY'ýýCOMPOS REQUIREMENT 4 30 EFFLUENT GROSS VALUE DAILY MX OIL AND GREASE SAMPLE ******** MEASUREMENT 19 00556 1 0 0 PERMIT' * *** * **

p ****~~ ~ 15 MG/L 'DAILY GRAB EFFLUENT GROSS VALUE REQU IREMENT'.

                                                                                                                                                                                                                '.DAILY       MX PHOSPHORUS, TOTAL (AS P)                          SAMPLE MEASUREMENT                                                                                                                                                                                  19 00665      1     0 EFFLUENT GROSS VALUE 0                             E      .RM IT
                                                                                                                                                           -        4,'I                                   tDAILY -MX MG/L 1DAILY-COPPER, TOTAL (AS CU)                             SAMPLE                       'k'k'* * "k* "k"#                                                                                           **   * *

MEASUREMENT 19 01042 1 0 0 RPERMvIT REQUIREMENT

                                                                          "'********.,           '-44 4                                                                  ..                                  1.0              MG/L                   -DA LY-     OOMPOS EFFLUENT GROSS VALUE DAILY       MX IRON, TOTAL (AS FE)                               SAMPLE                              ** * * *                                                  * *****k'A MEASUREMENT                                                                                                                                                                                  19
                                                                                                        .4444,,

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                                                                                                                                                                            ~

FLOW, IN CONDUIT OR THRU SAMPLE ** ** * ** * ** ** * * * *

                                                                                                                                                                                                                                         *-,lk TREATMENT PLANT                              MEASUREMENT                                                                            03
                                                                                                                                                                          '<"N" *******

50050 '1 0 0 PERMIT REPORT' REPORT. MGD ,t'4 "4' AILCTID r'AL EFFLUENT GROSS VALUE REQUIREMENT. 4,

                                                                -     KM&~AV'Gt                             J"DAILYN~X                      --                                                       LC       '           '-4 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER            I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel
                                                                                                                                                                           .                                 J[             " "        TELEPHONE                        DATE
                                                                                                                                                                   '                    ,             {j1"                    L Timothy P. Cleary                   properly gather and evaluate the information submitted. Based on my inquiry of the person or                  ,V.,.                 .S,--L.
                                                                                                                                                                                    ,,,1     ,"&,

persons who manage the system, or those persons directly responsible for gathering the Pnicipal Environmental Engineer 423 843-6700 08 01 14' Sinformation, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period 3320-Formon (RE 3/99) EPA Previous PreiouFom editions 33201may be(REV3/99) usedPae1o1 Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000

         " . (INTEROFFICE SB-2A)                                                                                      TN0026450                                        110 G                F-FINAL SODDY - DAISY       TN 37384                                                                           PERMIT NUMBER                        DISCHARGE NUMBER                   RECYCLED COOLING WATER Facility     TVA - SEQUOYAH NUCLEAR PLANT Location      HAMILTON COUNTY                                                                                                         MONITORING PERIOD                                      EFFLUENT I YEAR      MOI        DAY      I          YEARI MO I DAY ATTN: Stephanie A. Howard                                                                               From 1 07 1.12                   011 To 1 07 1 12 1 31                                       NO DISCHARGE              L      **

NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE I EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 04*04 CENTIGRADE MEASUREMENT 04, 00010 Z INSTREAM MONITORING 0 0 PERMT .C* DEGC . 383 DGC Ai* REQ-UIREMENTLI- 7... ,.., ' DAIL. .3,___, M. PH SAMPLE ******** ** ********12 MEASUREMENT 00400 1 0 0 PRT. 60 9.0iSU GRAB': EFFLUENT GROSS VALUE REURMN' ___ MINImum' ,+

                                                                                                                                                                                           ..      MAXIMUM______                                            ___

SOLIDS, TOTAL SUSPENDED SAMPLE ...... ** ******** 19 MEASUREMENT I 19 00530 1 0 0 PERM*I*T K*.,*.* 130 *. ** ": . ..,

                                                                                                                                                                                                            .i,     ?* 4            1'p !:_

CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ** ******** 19** MEASUREMENT 19 50060 1 0 0 PRMIT ***00MG/L WEKL RAB-EFFLUENT GROSS VALUE RE. IFM-N __10-, ALYM 0.,,_______ SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my (l, I Iy TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or . persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engtneer information, the information submitted is , to the best of my knowledge and belief, true, _ ______ ______ ___ _ 423 .843-6700 08 01 "_14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE

                                    .                information, including the possibility of fine and imprisonment for knowing violations.                       OFFICER OR AUTHORIZED AGENT                          AREA       NUMBER         YEAR    MO    DAY TYPED OR PRINTED                   .CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS                      (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUER 01) OMB No. 2040-0004 Address P.O. BOX 2000 (INTEROFFICE SB-2A) TN0026450 L 110 T F- FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT Y A Y-

I YEAR MO DAY NO DISCHARGE ATTN: Stephanie A. Howard From L1O0 12 To 07 I12 !3131 .1; f-1N40Ix.

1 U 1instruuciUos utnure UUrIIpoltiIIu Lsa rmUII. PARAMETER QUANTITY OR LOADING QUALITY OR CONCEN TRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA SAMPLE MEASUREMENT _sEW

                                                                                                                             **RE                                                                                      1*23  23 iTRP3B    1    0    0                              FERMI                                                                                          45,2                                                                    PRET                I'EL
                                                                                                                                                                      -1*                                                                                       CMO EFFLUENT GROSS VALUE                        '        .                                                                                        MINIMUM                                                _'_'.............{NNUAL.                         . ....        ..-  .    .

IC25 STATRE 7DAY CHR SAMPLE * **23 PIMEPHALES MEASUREMENT* TRP6C 1 1 0 0:RSEMI 0ER***.42 1 PERCENT COMPOSý

45. " **- ""r-. PERCENT REQUIREMENT -

EFFLUENT GROSS VALUE ... .- A AL _ _____MINIMUM ý'li)i :..I.>: ___ SAMPLE MEASUREMENT PEIRMIT -* SAMPLE MEASUREMENT REASUIREMENT PERMIT .,.

                                           ~REQU1REMENT                 ,                                                                                           c SAMPLE MEASUREMENT PERMIT                                                                                                                  -'

REQUIREMENT li~ 4 SAMPLE MEASUREMENT PERMIT-REQUIREMENT . SAMPLE MEASUREMENT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certity under penalty of law that this document and all attachments were prepared under my j, A If TELEPHONE direction or supervision in accordance with a system designed to assure that qualified personnel DATE Timothy P. Cleary property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 information, the information submitted is , to the best of my knowledge and belief, true, 843-6700 08 01 14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.. SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT I AR NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions maybe used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 (INTEROFFICE SB-2A) TN0026450 116 G F-FINAL

        - SODDY - DAISY       TN 37384                                                                                                                                                         BACKWASH PERMIT NUMBER                        [DISCHARGE NUMBER Facility   TVA - SEQUOYAH NUCLEAR PLANT Location   HAMILTON COUNTY                                                                                                               MONITORING PERIOD                                      EFFLUENT YEAR ý                 DAY               YEAR         MO IDAY0I]                    NO DISCHARGE ATTN: Stephanie A. Howard                                                                               From            07        1      11              TO 10              2j       31 NOTE: Read instructions before completincl this form, PARAMETER                                                                    QUANTITY OR LOADING                                                          QUALITY OR CONCENTRATION                                         NO.        FREQUENCY   SAMPLE EX             OF        TYPE AVERAGE              -~        MAXIMUM                   UNITS              MINIMUM                AVERAGE
                                                                                                                                                                                                'a-MAXIMUM            UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY)                    SAMPLE                                              ___________                     _____

0 0 1/31 VISUAL MEASUREMENT 9A 01345 1 - 0 0 PERM~IT * * * * ** *

PASS=0 :SEE REQUIREMENT .

REPORT EFFLUENT GROSS VALUE MOTOAL FAIL=1 I .-- -~ _ ___M___ _____ ___ ____- .'~ OIL AND GREASE VISUAL SAMPLE ** ** * **

0 0 1/31 VISUAL MEASUREMENT 94 84066 1 0 0 PERMIT -***** REPOR 1 YESurI SEE VISUAL EFFLUENT GROSS VALUE REOUIREMENIJT' R, T{' NO=O I______

{ MO TOTAL PERMIT. SAMPLE MEASUREMENT PEfRMIT REQUIREMENT: SAMPLE MEASUREMENT

PERMIT 1.

SAMPLE 4 4- ______ L 4.

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14 1 1" REQUIREMENT

                                                    "             4.1           1 4                    1<1     .1 '1            ~
                                                                    .1 4 NAME/TITLE PRINCIPAL EXECUTIVE                 I Certify under penalty of law that this document and all attachments were prepared under my
                                        -OFFICER                                                                                                                                            A     iTELEPHONE                                                  DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary                propedy gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the                                Principal Environmental Engineer                   .423        843-6700              08   01        14 Site Vice President               information, the information submitted is , to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false                       SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.                                OFFICER OR AUTHORIZED AGENT                        AREA       NUMBER              YEAR   MO      DAY TYPED OR PRINTED                                                                                                                                                                                            CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS                (Reference all attachments here)

Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320.1 (REV 3/99) Previous editions may be used Page 1 of I

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) - MAJOR Form Approved. Name TVA-- SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Address P.O. BOX 2000 (INTEROFFICE SB-2A) TN0026450 117 G F-FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER BACKWASH Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY _____ MONITORING PERIOD EFFLUENT ATTN: Stephanie A. Howard From F07 1-2_ -0holJ To 0 I 121! 31 !1 . NO-DISCHARGE YEAR L MO IDAY I YEAR I MO I DAY ODSCAG ' NOTE: Read instructions before completinq this form. PARAMETER AVERAGE QUANTITY OR LOADING MAXIMUM UNITS MINIMUM QUALITY OR CONCENTRATION AVERAGE MAXIMUM 2EX NO. UNITSANALYSIS 1 FREQUENCY OF SAMPLE TTYPE DEBRIS, FLOATING (SEVERITY) SAMPLE ** * ** 1/31 VISUAL MEASUREMENT 0 9A 0 01345 1 0 0 PERMIfT ** * * * * ** * * * *

REPORT PASS=0 -SEE VISUAL-

ýEFFLUENT GROSS VALUE REQUIREMENTV FAIL=I PERMIT ___________ ____________ ____________ I- ~ -~____________ '- MO'TOTAL I- t I-OIL AND GREASE VISUAL SAMPLE MEASUREMENT n 0 94 0 1/31 VISUAL 184066 1 0 iLEFFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT

                                                                                 -     *****      FREPORT                      YES=l NO=O SEE        VISUAL MO TOTAL                                                                                      I --

PERMIT SAMPLE MEASUREMENT

                                                 -PERMIT REQUIREMENT I                 -r

_______ F t SAMPLE MEASUREMENT

                                                 -- PERMIT-REQUIREMENTr

__________________________-------I- I- -r I I SAMPLE MEASUREMENT PERMITl REQU)LIREMENT

                                    -      i-      -          -    -        -       - __     _   _-  _   __   _  - _   _-__               I.--------------              i   -                      -t---                -    -   -l    1T     -T-7            -

SAMPLE MEASUREMENT l----------

                                                   -PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT, REQUIREMENT.

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my .t Timothy P. Cleary - direction or supervision in accordance with a system designed to assure that qualified personnel property gather and evaluate the-information submitted. Based on my inquiry of the person or I Lc.e persons who manage the system, or those persons directly responsible for gathering the Principal Envtronmental Engineer Pinformation, the information submitted is , to the best of my knowledge and belief, true, ___ Site Vice President accurate, and complete, I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED . COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previouseditions may be used Page I of I

PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) 2040-0004 (SUBR 01) OMB No.}. Address P.O. BOX 2000 (INTEROFFICE SB-2A) ITN0026450 I[118 G F - FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT YEAR MO* -DAY YEAR MOI DAY NO DISCHARGE @ ATTN: Stephanie A. Howard From l07 12 1 01 To! 07 12 31 NOTE: Read instructions before completincl this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE jE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MA IMUM ANALYSIS

                                            ><                                                                                                                                                                           UNITS                     F          TP OXYGEN, DISSOLVED             (DO)                  SAMPLE MEASUREMENT                                                                                                                                                                  19
                                                                  ..  ...       . ..       ....    ...                                                         -I.........................

00300 1 0 0 PERMIT * * ** * * *

MG/L TWICE!/ GRAB REQUIREMENT 2.0, EFFLUENT GROSS VALUE DAILY MN SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 00530 1 0 0 PERMIT *
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T GRAB EFFLUENT GROSS-VALUE REQUIREM,.ENT SOLIDS, SETTLEABLE DAILY MVX SAMPLE 25 MEASUREMENT 00545 1 0 0 P*RMIT 1.0 ML/L ONCE! GRAB1* REQUIREMENT EFFLUENT GROSS VALUE DAILYi MX MONTH. ______ FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 03 150050 1 0 " 0 PERMIT REPORT , RE PORT MGD l.

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PERMITr REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTEDI COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previouseditions may be used Page 1 of 1}}

ML080160592: Difference between revisions

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ML080160592: Difference between revisions

Latest revision as of 08:01, 13 March 2020

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Dear Mr. Patrick Cromer:

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