ML080160592
| ML080160592 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 01/14/2008 |
| From: | Cleary T Tennessee Valley Authority |
| To: | Cromer P Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control |
| References | |
| Download: ML080160592 (114) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 January 14, 2008 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR DECEMBER 2007 Enclosed is the December 2007 Discharge Monitoring Report for Sequoyah Nuclear Plant.
Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Stephanie A. Howard Principal Environmental Engineer
.Signatory Authority for Timothy P. Cleary Site Vice President Sequoyah Nuclear Plant Enclosure
.cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Prirced on recycled paper
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A)
SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard Different)
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN0026450 101 G__
F-FINAL PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE F
MONITORING PERIOD EFFLUENT Form Approved.
OMB No: 2040-0004 YEAR I MO I DAY I MO*DA NO DISCHARGE From 07 I12 I01 I.To 0 2
3 NOTE: Read instructions before completinq this form.
QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCYL SAMPLE EX OF TYPE ANALYSIS PARAMETER TEMPERATURE, WATER DEG.
CENTIGRADE 00010 Z
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NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Q
L Timothy P. Cleary property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Pnncipal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, PrincipalEnvironmental_
423 843-6700 08 01 14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 2 EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 2
DMR Attachment CCW Data CCW TRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/13/2007 @ 1215 0.33 mg/L 12/16/07 @.1854 RDH TN EPA 8015 CCW CHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/13/2007 @ 1155
<0.10 mg/L 12/16/07 @ 1905 RDH TN EPA 8015
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 L(NTEROFFICE SB-2A)
SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN0026450 101 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Form Approved.
OMB No. 2040-0004 I
I MONITORING PFRIODl I
EFFLUENT ER MO DA YEAR MO DAY NO DISCHARGE NOTE: Read instructions before completinp this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
EX FREQUENCY OF ANALYSIS SAMPLE TYPE 1~
-r AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS CHLORINE, TOTAL RESIDUAL 50060 1
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EFFLUENT GROSS VALUE TEMPERATURE - C, RATE OF CHANGE 82234 1
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SAMPLE MEASUREMENT NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Ceriify under penally of law that this document and all attachments were prepared under my i
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TELEPHONE DATE
-direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gatheringthe Principal Environmental Engineer 423 843-6700 08 01 14 Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, 423_843-6700_08_01_14 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE Sinformation, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
The following injections occured: 1. Biodetergent 73551 (max. calc. conc. was 0.022mg/L--limit 2.Omg/L)
EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 2 of 2
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A)
SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard PARAMETER I
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUB R 01)
TN0026450 101 Q F-FINAL PERMIT NUMBER DISCHARGE NUMBERE DIFFUSER DISCHARGE MONITORING PERIOD EFFLUENT YEART MO DAY I YEAR MO NO DISCHARGE From 07 1 10 01 To N21 3 1 ReNO i ns trucH t
NOTE: Read instruction!
Form Approved.
OMB No. 2040-0004 11:1 s before comoletinq this form.
QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE I
IANALYSIS AVERAGE MAXIMUM UNITS
<n 9nf n
1 /92 BORON, TOTAL 01022 1
0 0
EFFLUENT GROSS VALUE SAMPLE MEASUREMENT REQUIREMENT SAMPLE MEASUREMENT EOU IRE MENNT NAMETITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE directin or supervision in accordance with a system designed to assure that qualified personnel
,47'J A 9~L)iX Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Sinformation, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 08 01 14 Site Vice President accurate, and'complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I
A N
_information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Boron was sampled on 10103/2007 @ 1328.
EPA Form 3320-1 (REV 3/99)
. Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name "TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A)
SODDY-DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR For DISCHARGE MONITORING REPORT (DMR)
(SUR 01)
O TN0026450 11 T
F-FINAL PERMITNUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 MONITORING PERIOD EFFLUENT m Approved.
lB No. 2040-0004 IYEAR MO DAY I YEAR MO_ý DAY From 07[ I 12 01 To 07 12 31 NO DISCHARGE NOTE: Read instructions before completinq this form.
PARAMETER QU AVERAGE PLE EMENT ANTITY OR LOADING QUALITY OR CONCENTRATION NO.
EX FREQUENCY SAMPLE OF I
TYPE ANALYSIS MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMF CERIODAPHNIA MEASUR TRP3B 1
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary Site Vice President TYPED OR PRINTED I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel
.t 4AL[
properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer nformation, the information submitted is, to the best of my knowledge and belief, true.
accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.
OF FICERr OR9 AU TIOTHORIZED AGEN-4T COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was sampled 12/02/2007 - 1210712007. Report is attached.
EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 1
°
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: Januaa 11 200 Facility / Discharger: Sequoyah Nuclear Plant /TVA
- 2. County /State:
Hamilton / Tennessee
- 3.
NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5.
Design Flow (MGD): 1579
- 7.
1Q10: 3491
- 8. OutfallTested: 101
- 9. Dates Sampled: December 2-7, 2007
- 10.
Average Flow on Days Sampled (MGD): 1573, 1575, 1577, 1578, 1577, 1574
- 11.
Pertinent Site Conditions: Towerbrom 960 was injected into ERCW A & B Trains and RCW. The dates and times for the Towerbrom 960 injection are in the following table.
See Appendix B for complete additional chemical application information during the sample collection period.
Date/Start Time Date/Ending Time Injection Location(ET(S)
(EST)
(EST)
ERCW A & B and RCW 12/02/2007 @ 0001 12/07/2007 2400
- 12. Test Dates: December 4-11, 2007
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia)
- 15. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephales promelas: UV treated Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0 Page 1 of 101
- 16. Permit Limit Endpoint (%): Outfall 101: IC
= 45.2%
- 17. Test Results: Outfall 101: Pimephalespromelas: LC25 > 100%
Ceriodaphnia dubia: IC 5 > 100%
UV treated Outfall 101: Pimephales promelas:IC25 > 100%
- 18. Facility
Contact:
Stephanie Howard Phone #: (423) 843-6700
- 19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Cynthia L: Russell Phone #: (256) 386-2755
- 22. Notes: Outfall 101 samples collected December 2-7, 2007, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >
100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant differences from the control during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Page 2 of 101
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101,Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM/DD/YY)/
(MM/DD/YY)/
Arrival Initial (MM/DD/YY)/
Sample Time (ET)
Time (ET)
Temp.
TRC*
Time (ET)
ID Collected Received
(°C)
(mg/L)
Last Used By 101 12/02/07 0738 to 12/03/07 1434 2.7/3.1'
<0.10 12/04/07 1250 12/03/07 0638 12/05/07 1156 12/02/07 0807 to 12/04/07 1250 Intake 12/03/07 0707 12/03/07 1434 2.4
<0.10 12/05/071156 12/04/07.07527t 12/06/07 158 101 12/04/07 0752 to 12/05/07 1325 1.7/1.2'
<0.10 12/06/07 1158 12/05/07 0652 12/07/07 1155 12/04/07 0824 to
.12/06/07 1158 Intake 12/05/07 0724 12/05/07 1325 2.2
<0.10 12/07/07 1155 12/05/07 0724 12/07/07 1155 12/08/07 1209 101 12/06/07 0750 to 12/07/07 1350 1.5/2.0'
<0.10 12/09/07 1311 12/07/07 0650 12/1.0/07 1300 12/08/07 1209.
Intake 12/06/07 0806 to 12/07/07 1350 0.4
<0.10 12/09/07 1311 12/07/07 0706 12/10/07 1300
- TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 :L 1.0 C0 in a warm water bath.
Aliquots of Outfall 101 and Intake samples.were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.
Page 3 of 101
Pimephales promelas Test Organisms:
Ceriodaphnia dubia In-house Cultures
<24-hours old
- 1. Source:
- 2. Age:
Aquatox, Inc.
20.75 hours8.680556e-4 days <br />0.0208 hours <br />1.240079e-4 weeks <br />2.85375e-5 months <br /> old Test Method Summary:
- 1. Test Conditions:
Static, Renewal Static, Renewal
- 2. Test Duration:
7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic
- 3. Control / Dilution Water: Moderately Hard Synthetic
- 4. Number of Replicates:
4
- 5. Organisms per Replicate: 10
- 6. Test Initiation: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 7. Test Termination: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 8.
Test Temperature: Outfall 101:
10 A
I 12/04/07 1250 ET 12/04/07 1237 ET 12/11/07 1200 ET 12/11/07 1145 ET Mean = 24.6°C (24.1 - 25.00 C) 12/04/07 1045 ET 12/11/07 1009 ET Mean = 24.8°C (24.5 - 25. 10C)
Test Temperature: UV-Treated Outfall 101:
Mean = 24.6°C (24.1 - 25.1 °C)
- 9. Physical / Chemical Measurements:
Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics:
Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
Page 4 of 101
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted December 4-11, 2007 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3
[4 5
6 7
Control 100 100 100 100 100 100 100 11.3%
100 100 100 100 100 100 100 22.6%
100 100 100 100 98 98 98 45.2%
100 100 100 100 100 100 100 72.6%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 98 Mean Dry Weight (mg)
Test Soluetis (replicate number)
(%Effluent) 1 2
3 4
Mean Control 0.804 0.535 0.540 0.671 0.638 11.3%
0.718 0.745 0.740 0.722 0.731 22.6%
0.709 0.651 0.632 0.737 0.682 45.2%
0.673 0.596 0.556 0.652 0.619 72.6%
.0.657 0.628 0.627 0.643, 0.639 100.0%
-0.582 0.557 0.658 0.624 0.605 Intake 0.626 0.699 0.522 0.690 0.634 IC25.Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc
- 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5 0: TUc = 100/IC25 Page 5 of 101
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Resul6 of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted December 4-11,2007 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions 1
2
(% Effluent) 4 5
6 Control 100 100 100 100 100 100 100 11.3%
100 100 100 100 100 100 100 22.6%
100 100 100 100 100 100 100 45.2%
100 100 100 i00 100 100 100 72.6%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Tet Soluetis Data (replicate number)
(%Effluent) 1 2
3 4
5 6
7 8
9 10 Mean Control 31 32 35 33 27 34 31 34 32 30 31.9 11.3%
33 37 33 34 37 30 34 33 31 33 33.5 22.6%
35 31 35 37 37 36 32 33 36 36 34.8 45.2%
34 38 35 39 35 35 36 40 36 33 36.1 72.6%
38 36 40 35 36 39 35 38 38 36 37.1 100.0%
38 36 41 38 38 41 36 38 37 41 38.4 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/IC25 Page.6 of 101
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted December 4-11, 2007 using water from Intake Percent Surviving Test (time interval used - days)
Solutions 2
I3 4
5 67
(% Effluent) 2 3
4 5
67 Control 100 100 100 100-100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
(t Eflueont)
Data (replicate number)
(%Effluent) 1 2
3 4
5 6
7 8
9 10 Mean Control 30 29 30 31 29 33 36 31 31 31 31.1 Intake 39 33 37 37 33 36 33 38 37 35 35.8 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA
.Lower Limit: NA
_*TUa = 100/LC5 o: TUc =100/IC25
(
Page 7 of 101
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted December 4-11, 2007 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
I 7
Control 100 100 100 100 100 98 98 11.3%
100 100 100 100 100 98 98 22.6%
- 100 100 100 100 100 100 100 45.2%
100 100 100 100 100 100 100 72.6%
100 100 100
.100 100 100 100 100.0%
100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg)
Test Solutions (replicate number)
(% Effluent) 1 2
3 4
Mean Control 0.682 0.556 0.830 0.808 0.719 11.3%
0.609 0.664 0.687 0.724 0.671 22.6%
0.801 0.661 0.730 0.767 0.740 45.2%
0.716 0.659 0.599 0.766 0.685 72.6%
0.692 0.688 0.789 0.643 0.703 100.0%
0.650 0.603 0.702 0.643 0.650 Intake 0.691 0.711 0.736 0.698 0.709 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/ IC2,5 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species I
Date Time Duration Toxicant Results (IC25)
Pimephales promelas December 4-11, 2007 1307 7-days KCI 0.52 g/L Ceriodaphnia dubia December 4-11, 2007 1035 7-days NaCI 1.07 g/L Page 8 of 101
Water Chemistry Mean Values and Ranges for Pinmephales promelas and Ceriodaphnia dubia Tests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101, December 4-I1, 2007.
Test Sample 11)
Temperature ('C)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Alkalinity Hardness Total Residual Initial -
Final Initial Final Initial Final
(ýLmhos/cm)
(mg/L CaCO3) (mg/L CaCO3) Chlorine (mg/L)
Contiol 24.7 24.5.
7.6 7.5 7.91 7.75 312 57 93 24.6 - 24.8 24.3 24.7 7.4 7.7 6.8 8.0 7.38 8.15 7.56 -
8.05 296 337 57 57 92 94 11.3%
24.7 24.4 7.8 7.5
.7.85 7.74 303 24.6 24.8 24.2 24.5 7.4 8.2 6.9 7.9 7.57 -
8.09 7.53 -
8.01 287 318 2.%
24.7 24.5 7.8 7.5 7.87 7.75 297 22.6%
24.6
- 24.9 24.4 -
24.6 7.6 8.0 6.9 7.9 7.56 -
8.09 7.53 -
8.06 280 -
320 24.8 24.5 7.9 7.5 7.87 7.77 277 45.2%
45.2%
24.7
- 24.9 24.1
- 24.6 7.6 8.1 6.9 7.8 7.56 -
8.09 7.52 -
8.09 255 307 7Z 24.8 24.5 8.0 7.5 7.88 7.80 254 72.6%
24.7
- 25.0 24.3. -
24.6 7.6 8.2 6.9 7.8 7.54 -
8.13 7.53 -
8.10 241 268 100.00 24.9
- 24.5 8.0 7.5 7.89 7.83 234 72 87
<0.10 24.7
- 25.0 24.3 24.7 7.7 8.2 6.9 7.9 7.51
- 8.14 7.56 -
8.13 220 -
250
- 68.
76 78 98
<0.10 -
<0.10 Intake 24.9 24.5 8.0 7.5 7.87 7.82 233 73 89
< 0.10 24.7
- 25.0 24.4 -
24.7 7.7 8.4 6.9 7.9 7.48 8.13 7.58 -
8.09 220 251 70 74 84 94
<0.10 -.
<0.10 Control 24.7 24.7 7.6 7.8 7.91 7.91 312 57 93 24.6
- 24.8 24.5 -
24.9 7.4 7.7 7.5 8.1 7.38 -
8.15 7.58 -
8.07 296 337 57 57 92 94
- 1. %
24.7 24.8 7.8 7.8 7.85 7.92 303---
24.7 24.8 24.5 24.9 7.4 8.2 7.5 7 8.1 7.57 -
8.09 7.57 8.08 287 318 24.7 24.7 7.8 7.8 7.87 7.92 297 2 2.6 IV 24.7 -
24.8 24.5 -
24.9 7.6 8.0 7.5 8.2 7.56 -
8.09 7.57 -
8.08 280 -
320 4.%
24.8 24.7 7.9 7.9 7.87 7.94 277 4:
45.2%IV 24.7 -
24.9 24.5 -
25.0 7.6 8.1 7.6 8.2 7.56 -
8.09 7.56 -
8.11 255 307 24.8 24.8 8.0 7.9 7.88 7.97 254
.2 72.6%
24.7 -
25.1 24.5 -
25.0 7.6 8.2 7.6 8.2 7.54 -
8.13 7.57 -
8.16 241 268 24.9 24.8 8.0 7.9 7.89 7.99 234 72 87
< 0.10 24.7 -
25.1 24.6 -
25.0 7.7 8.2 7.7 8.1 7.51 8.14 7.57 -
8.19 220 -
250 68 76 78 98
<0.10 -
<0.10 Intake 24.9 24.8 8.0 7.9 7.87 8.01 233 73 89
<0.10 24.7 -
25.1 24.7 -
25.0 7.7 8.4 7.6 8.1 7.48 -
8.13 7.62 - 821 220 -
251 70 74 84 94
<0.10 -
<0.10 Overall temperature CC)
Pimephales promelas Ceriodaphnia dubia Average 24.6 24.8 Minimum 24.1 24.5 Maximum 25.0 25.1 Page 9. of 101
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the Pimephales promelas Test, UV-treated Sequoyah Nuclear Plant Effluent (SQN),
Outfall 101, December 4-11, 2007.
Test Sample ID Temperature CC)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Alkalinity Hardness Initial Final Initial Final Initial Final (gmhos/cm) (mg/L CaCO 3) (mg/L CaCO3)
Control 24.7 24.6 7.8 7.6 7.86 7.75 313 57 89 24.6 - 24.8 24.3 24.7 7.6 8.0 7.1 7.9 7.61 -
8.08 7.55
- 7.98 294 333 57 58 88 92 11.3%
24.8 24.4 7.8 7.6 7.87 7.73 309 24.7 -
24.9 24.2 -
24.6 7.7 7.9 7.0 7,9 761 8.08 7.53 -
7.97 288 329 24.9 24.4 7.8 7.4 7.86 7.76 295 21.6%
7.3
-80 27
-31 24.7 - 25.0 24.1 24.8 7.7 7.9 6.8 7.9 7.60 8.10 7.53 8.00 277 311 24.9 24.4 7.8 7.5 7.87 7.77 281
.*45.2%4.
24.7 -
25.0 24.2 -
24.8 7.6 7.9 6.6 8.0 7.55 8.12 7.53
" 8.03 259 305 726%
24.9 24.4 7.9 7.5 7.88 7.82 256 72.6%
24.7 -
25.1 24.1 24.8 7.8 8.1 6.6 8.0 7.55 -
8.15 7.55 -
8.05 236 278 100.0/
25.0 24.4 8.0 7.5
. 7.89 7.83 235 69 90 24.8 25.1 24.1 24.6 7.8 8.2 6.8 8.0 7.52 -. 8.18 7.57 - 8.09 219 249 66 72 86 94 24.9 24.4 8.0 7.6 7.89 7.83 234 66 89 Intake
_____24.8 25.0 24.2 24.6 7.7
-8.3 6.9
-8.2 7.52 8.16 7.59 8.08 215
-256
- 60) 70 84 94 Overall temperature ('C)
Pimephales promelas Average 24.6 Minimum Maximum 24.1 25.1 Page 10 of 101
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected December 2-7, 2007, showed no toxic effects to fathead minnows or daphnids:- The resulting IC 25 values, for both species, were >
100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant differences from the control during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Page 11 of 101
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas-Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-82 1 -R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None DEVIATIONS IMODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None Page 12 of 101
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants,. Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B.
- 5.
The pH was measured by SM 4500 H+ B.
- 6. Conductance was measured by SM 2510 B.
7; Alkalinity was measured by SM 2320 B.
- 8.
Total hardness was measured by SM 2340 C.
- 9. Total residual chlorine was measured by ORION Electrode Method 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCI or NaCI.
- 2.
Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephales promelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4.
Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 13 of 101
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3.
Standard Methods for the Examination of Water and Wastewater, 2 0 th Edition, 1998.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14of 101
Sequoyah Nuclear Plant Biornonitoring December 4-1.1, 2007-Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser-Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling Page 15 of 101
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1.998 -December 7, 2007 Towerbrom mg/L TRC PCL-401 mg/L Copolymer Cuprostat-PF mg/L Azole 0.005 0.011 0.021 0.019 0.015 0.015 0.015 0.024 0.024 0.024 0.024 0.024 0.024 0.023 0.015 0.012 0.023 0.022 0.022 0.025 0.016 0.120 Page 16 of 101
Table 13-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbio logically Induced Bacteria and Mollusks,
~During Toxicity Test Sampling, March 12, 1998 - December 7, 2007 Date
!::ii::iiiil*i!ii::iiii:iii:Towerbrom ::: 22':::::::::::::
PCL-401
- i:
- i
- : Cuprostat-N4 1OW:
01/31/2000
< 0.002 0ý026!ll~i iiiiiiiii 0.009 iiiiiiiii iiiiiiii i 0 2 /0 1/2 0 0 0 :::::::::::::::::::::::::::::::::::::::::::::::::
0.0 11 0,0:2:6iii:i
- ii:iiii:i:: 0.0 2 8 iiiiii i
!*i!l 02/05/2000 iiiiii}i}iiiiiiiiiiiiiiiiiii:iii
< 0.002 0,027iiii~
~ ~iii!iii~:i 0.009 i:17 7:iii!iiiiiiii~i~~il~iii!l
< 0. 0 5 7 ::::::::::::::::::::::
- : :::::::::::::.0 0.0 19 0 7 3 2 :::iii~i::iii:iiiiiii{
!iiii i::i~ii*:iiiiiii
< 0. 0 6
- : : :::::::::::: 0. 1
- 0
............0...........
1 2 1 / 0 0.........
0.
1 4
- 0..
5............
A.......02 0*i!!i**
~
~
i!ii:i* :i:*::*#
12/14/2000 #* * * * # 3 33 < 0.0087
.3i{*((((!{
i*
- ((i{!3!{
2 i
+ :-........... -........---
- ) :::: i:: !:: ii:: ii 12 15 2 0 0 ~i~i~i~iiiiiiii~i!!**i*{~iiiiiii!ii::iii!ii*i~~i 0 0 1 0
.[~iiiiiii~ *
{[ii!.[I 0. 20
{....
- .:.:*i:*iiiiii:i:i~i::i 12/16/2000
<iii~iiiiiiiiiiii~iiiiiiiilii:~iiii
<0.0036 i~iiiiii{**iii~ii{i*
0.020 iii~ li i**i~
082/208/27/2001
<0.0096 0.021 2:**}ii****
<.09
- 3;i~N i!i*
0.2 ii}**N~i{i}i ii~l*i* !!i{ 0 8/2 8/200 1 iiiiiiiiiiiiiiiiiiii((((iiiiiiii}i < 0.0 12085 iiiii((~i i iiiiii~iii 0.02 1 ~~iii!!!! i}:iii~~iii!!(( 08/29/2001 iiiiiiiiiiiiiiii;iiiiiiiiiiiii)iii! "<0.00!94 [iiiiii~ * }ii{i..~ 0.020 --0{~ii* N ii*iiif 00.5i~iii~~ii;i{!{~i .---....--..-. '...-.-...-., w*.. -,. = =
== =
.===== -======
E m m a::)::*:!;::i::): 1 2 / 1 0 /2 0 0 1
- =*:::::=::=:::::::
0 0 4
===========
===========================iiiiiiii{ii i{~iii!iiii ~iili iiiiiiiii 12 /1 2/2 0 0 !3iiiiii!*iii{*{*{}i ~i~::i(( 0. 1 2
- ..0......{ii* *
{i~i((ii i!!i~i(({*ii*!~i!ii!!ii 11/26/2001 <0.0 1 19 0.02 0.05iiiii)iiiii!)il <0 0 8 . 2
- 333333**33:****333333-ii~:i::;:i::{::i:}:::!ii ii ii~
1 2 1 /2 0 l
- iiiiii=:~i. 1 3 i~*!!
j* { iiiiiii~!! . 2 iii~iiiiiii ii~i}~iii Page 17 of 101
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 7, 2007 Date Towerbrom..,..,,.. PCL-401 CL-33 Cuprostat-H-43 M: M hlne mg/L mg/i..1 mg/L' Mgf-L PP gF mgLTRC Phosphate Copolymer DTMAD mg/L Qa Azole 01/02/2002 < 0.0079 0,G23 0.02 0J)06 01/03/2002 < 0.0042 0,.023 - 0.014 01/04/2002 0.0124 0, Q2 4 0.014 --0,009 i~ ii~ii:ii 05/09/2002 f4.057i}:*i{*i!{*i~!{i**i:i*iii*}i}* < 0 0 42
- i::i::::
- :::ii:::i:i:::
0.00014.iii{:*i::*::*:{:i~~~i:i ~i!:ii~!*iiiiiii:l 08/05/2002 iiiiiiiiiiiii!ii* i:i{iiiiiiiiiii < 0.0058
- ~~iiiiiiiiiii~*{~***l~ii~ii PO 5$.
0.018.iii(({iiiii{::::::::::::i::::::::::::::::::
- i:? i:i:i:ii!{
ii# i: 01/06/2002 < 0.0092 3 0.018 08/07/2002 < 0.01072 ..05: 0.1 ":0 08/08/2002A <0.00691: t5 .1 102/06/2002 < 0.0049-10/07/2002 0.0153 008 00 102/08/2002 <0.0042 . ~~5 0.018230 102/09/2002 0.01243
- Q3 0.018200 101/020.0013
'023 -- 5 -- 0- 1 00 02/21/2002 <0.0042 0.018 01/2/003<0003 01/13/2003 <0.006 5019 <.00004:04000: 01/014/2003 <0.01 0,0.02 01/125/2003 <0.0043 0.020 .0.009 04/06/20032007 04/06/2002 <00189 0.02 0 1 4.-ý-ý 04/08/2002 < 0.001 0.021 04/09/20032003 0.021 .1 04/10/2003 <013 .0219 .0.1 /04/1 /2003 <0.007 8 0 5 / 5 / 0 0
- *; ii#i 0 8 /0 6 /2 0 0 2
- ii:::i)::::ii:
-?!!}*!!iS5:5! 0 8 /0 7 /2 0 0 2 < 0.0 107i::ii1:iii:i{:~i*
- ii::~iii:::i:i:::*::
- iýiý'
- ii::~iii:::i:i:::*::
ii}ý:!:*iiiii........ . 19
- !**ii:o:*:**
- i:*:*:*:
i ......::i~i:**:*::*iii::i{: 108/06/2002 <0.0 104 7 .i~iiii~ ~ ~~i:iiii:iii iii~i iiiii
- ~i:i::i:i:i.i:i:::{:*i:i 1 0 /0 7 / 0 0 2.00 1 5 3
.'.-.','..-.----.-'..ý-... 0:::::::::::0::1 8:::::::
- 9::::::::
108/0 8/2 0 0 2..................................... < 0.0 0 6 19 2
- 0::::::
- 0 1:::::::::::::::X.0 9
10/10 /2002 :;ii{i!ii**iii:ii*ii!i}iiiiiii!iii:i.*ii*i <0.0134 0 -4ii~lliii{ iiii~i~i 0.018 J0,ii~ l~ i{*!!~ !iiii~~i{*iiiii{~ 010/12/20023 { <.0 4 ((i(((({* * (({i(({:{ 0.0018
- (({~iii~i~*{:i*!{*((i
((((!;((ii((i{i 01101 0/13/2003 <0.006 0.019
- A 0.:i iiii:iii :i:! <0 0 6 i~:i:i:iii:i 2: iiiiiii~i
. 19::::::::::::::::::::::::::::::: x....... 1 8 0 1/14/2003 < 0.0 1 1 0.....~~~**:iiiiil~ ~l<*!ii***: i*** 0 4 / 9 / 0 0 ii~i(((({i(({**}i*i~i*{ i~*{i*{ii:*ii*:*:iiiiii 0.0 3 9
- i1::::::::::::::::::::::::::i::::
0. 2 1 !iiiiiii~ ~ iiiiiii,.,....,,
- i:ii:.............1i??}i!;::
04 1 /01/15/2003 -ý0.0063 0 0.020i~iiii~iiilliil <. 1 3 iiiiiiiiiiii~!i:iiiiiii{i!iii.{ 0 0 1 iiiiiii i !i~ii !i!ii~iiiii*iiiiii~l:i `* /* /2 3 ** iii**:*;;::i**:::i.::*
- iiii~ii**:!!i:!::*:**i
<.0 7
- ~iiiiii~i{!i~~!!i~!{!((ii{iii*ii:ii:...
0 0 2..,,,,,,,,, ,,,.!;.i-:ii~i:{i:i{iiiii~ii:: ~::{::ii~i::i~: Page 18 of 101
Table B-I (continued). S equoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 7, 2007 Date Sodium Towerbrom PCL-1222 PCL-401 CL463*36'1ý Cuprostat-H-10*116A4 Hyluichlo*ite mg/L rng4. mg/L fng/I.,L. PF mgtL mfi:L TRC Phosphate Copolymer
- -DMN'lAD mg/L Qa*a:
IRO Azole 06/15/2003 < <0.0045 06/16/2003 <0.0037 0,057 0.020 R022 06/17/2003
- 0.0048 0.041 0.014
,4 02/05/2004 < 0.00348. 0141 0.1 0092 05/04/2004 < 0.0128502 0.02900.2 il~iiii{:::
- i~ i!!((i~ii:i~..:...............
06/20/2003 <0.0048 0.t26 0.014 0000 0.025 05/06/2004. .0 .014 0 08/03/2003 <0.0050 0........**..0 0.02... 05/09/2004 <0.01504 0.& 0.020 0 / 4 2 47 0 08/05/2003 <0.0085
- 1 0.020 0 0 07/06/2004
<0.0077 0.0200,2 08/07/2003 0.0152 0.4157 0.020 19: 24 0/07 /2003 0.0223 0*057 0.020 0009 10/09/2004
- 0.
<0.001 3 0.020 0009 10/108/2003 <0.0106 x 0 1 5.7;ii}*}iiiiiiiiiiii!{!~*3 0 0 83 0.020
- : ::::::}i:i:i{:i:::::iiiiiiiiii 0 2 0 1 2 0 4 :::::::::::::::::::::::::::::
. 0 9 ~ i~ iiiii i iiiiiii*i~i 0 0 9
- 3*:::***:::**:::***:.............
0210 00/09/2003 0.0181 0.022i!!i!!!*i**i*~i <.0 4 iii~iiiiii~ * *~iiis!!ll .0 9 ili~~iiiii~iiiii
- ii~iiiiiiiil~iiiiiii
- ::**:i: :::::::i**::
10/10/2 003 ::*::**:*:*:iii~*:i
- ~*:i::*:iii*::*::*:*
0 0 3 ~ ii::::i:::!*
- 2 :iii~ii::i:
0.01 30.0 24**~~iiiii~i*i1i_:::::::::::::::::::::::::::: 02/ 4/ 00 )i~iiiiiiiiii**i~i~ii!i**ii~iiiiii~~iii~iii!! 0 0 1 4 iiiiiiiii !*
- i*{iii~i 0.0 9 iiiiiiiii~ii 02/02/2004 ii!iii*ii*ii:iiiiiiiiiiiiiiiii{iii
<0.0034 0,020i*!ii! iiiiiii~i 0.0140:9::::: : :::::::
- ~iiii
{iii 0 2 /0 4 /2 0 0 4 *iiiiiiiii5iiiiiii* {iiiiii*iii}{lii 0 0 1
- iiiiiiii i i:}:iiii:l 0.012 40 0 9 ii:i:i::::i:i:::
ii~iiii:::i~ ii~iiiiii:* 02/0 6/2 004 Fii!!*!*!ii~*!iiiiiii~~~iiiiiiii*iiiiiii 0.0 1052 c.ii i ii!iiiiii~ 0.009 iiiiiiiii::3*3# !*::}iil i a t::::i
- 0 /0 /2 0 4
- i:*:i:i:ii!i~ii::iiii:i~
iiiii:ii:{i:ii:{ii*i:::i* 0 0 2 3 ~ ii((iii((i i~ iiiiiii~!{li.0 9 ii}!ii i~ iii(({::::::::.:..::::::.::::..::.:
- ===========
0 7 / 9/ 0 0 **ii*.*:**i:.i~i::):3:)i:::**ii3*)*::*)3:}**:3i:: 0 0 1 2
- iiiiii~*::*~
i i~ i*iiii~ii~ii.0 0
- iiii::::!*i..:........-::...:*: * >3*
- -::*-:::i*::i*:
- i~:<
- 3*::*
- 3:;*:;#
-*.# *33133-;3;33333
- 33335
- # ;33# **!3 Page 19 of 101
Table 13-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 7, 2007 Date sodmm-*..:*
- !ii Towerbrom P(M::i
- 12'i!
PCL-401 CE*-(A***'!:- Cuprostat-PF R :101ii Nalco H ti~i Hyocllrtý mg/L rnviiiii~ i~il-iii mg/L t i;i* ii~i:i mg!L iii* !;) 73551 mj*~ii 11/0 7/2 004 iiiii*!ii~**~~*ii!{ii*iiii~iiiiii~i < 0.0 187 0,M):::::::::::
- 0.0 104iii!**:!i*!~ i~
....:i~iii:*:i:!i:i:*# 1 1/12/2 0 04
- }ii~~~~~*
ii:ii;::i::i::::i: <. 2 3 il:iiii:i i
- i~
- }::}:: 0.0 176
- i:iiii~:i::
02/09/2005 .....}::::::::::::::::::::::::::::::::: <0.0 042
- i:ii:ii:~ 028 :ii:::::i:: 0.0 10
- !*::*~i:*::::*:
02/07/2005 i!iiiiiii~i!i~!iiiiiiiiii <0.004 116 Q Q2S...
- ii~ii}i~i 0.010 0.007~!:*i:ii!i*i*!ii:i~ii~ii*i!
- i~iii**ii*
02 11 20 5 ii~iii}**:iii!iiiii**i!ii~iiiiii
- 0. 15
- }}*iii~ *) *!iiiiiiii 0 0 1
- }i~i~~ii~i~i~*!
iii!:*i~...........0.0.*li!i!!i~il 0..../.08..../....--,.-.... 0.,... 0..:080::::::::::::::::::......:::::::::::::::::::::::::::: 0 6 0 / 0 0 i:ii;:ii::i:i::ii:i i:iii::::i:i::i:ii 0 0 6 3
- i::**:;:i*
- i i::ii
"'.....................0..................... 02 0 /2 0 0.0::::::::::::::::::9::::::: 0.0 10********i:::::::::::::
- i*i~ ii~ ***ii~
iiiii??ii~**! ii-
- i~i~ii~~
0 6 0 6 2 0 5 }}:ii:i:i:}~~iiii:i i:::iii::iii~i:i:.0 4 3 i:::*:i:*:*::::*:*:*:*.:.4:::*:**}:*~~i)*
- ::::::::::::::::::::::}::::::::X X......
- j:::
- iiiii f 02/10/2005::::::::::::::
<0.0042 0.010:::
- ~
~ ~iii~ ii~~# i i!# i! i!iiiiiiiii.............. 02/ / 0511/2005 0~iii*!iiii!**!i! . 2 5 i!:::!::ii::::iiii':i:)i:ii!!::~:i:}~~~lii!ii*;}i~~~i~!i iiliiiii}i}ii!!!~}.0155........10 0.007ii 0 6 0 /2 0 .0....0....0....6 3..
=========
06/206/2005 :***i})*i**~~}i{ii 0.020943
- i}iiN }}i**.o
~1 .:..:.:::..::.::.::.::..:......!**!i!!!ii~ii~i
- !?:?::![
0 7 2 /2 0 iiiiii{i~iiiiii ii~i::ii~.......04 2......... 0 9........:::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::
- i~i:i?{i:i:
0 7/22 /2 0 0 5 ((ii~*((ii~i{*i~{*~i* 2 8 {i i }*ii~i~!i~~. 1 (({:~{i{i}((4{i((ii((((: . 14:::::::::::::: 06/07/2005:::::::::::::: 0.0103::::::: 1 1111 1111111111111
- i:i.i.:.:i:i ii.7.i1.....
0 6/0 8 /2 0 0 5 i{!i!iii{iii iii:i((iiiii 0.0 2 9 5
- j::: :::: :::}}:::::::::::::::
iii*~* !i!!** i* .::::::::********i* 2
- i**:~ ~iii 1 1 1 5 2 0 5 iiiiii:iiiiii:i:i
!::iii:iiiiiii~l. 2 5
- 2
- -*::}::::::::::....
- iii*i::
}:*~i:*i:*i:
- i*::::*:*:*:*:*
i:*~
- i:***ii}::*~
~;*:i i~ ~i * * # i 1 1 1 /2 0 i........~ ~iii~ iii::i{iiiii~i 0.0 1 8 4 .:i{i:iii:}i~{.*ii:iiii::i::i::i
- ~*;
- i
- *~i*:i:*::
- }::::
}}:::}::::::::: 06/10/2005
- ...:.:++.:..:.:.:.:..:::::::::
Page 20 of 101
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 7 December 7, 2007 Date Sodium Towerbrorr 4Hyp ohiote mg/L -g/L TRC 11/12/2006 0.0055 11/13/2006 0.0068 11/14/2006 0.0143 11/15/2006 0.0068 11/16/2006 0.0267 11/17/2006........... 0.0222 11/26/2006 0.0188 11/27/2006 0.0138 11/28/2006 0.0120 11/29/2006 0.0288 11/30/2006 0.0376 12/01/2006 0.0187
- 05/28/07 05/29/07 05/30/07 0.0084 05/31/07 0.0103 06/01/07 0.0164 06/02/07 0.0305 12/02/07 0.0241 12/03/07 0.0128 12/04/07 0.0238 12/05/07 0.0158 12/06/07 0.0162 12/07/07 0.0175 PCL-401 C L-3:
Cuprostat-PF mg/L mg/ilt' mg/L Copolymer D)MAD Azole Nalco 73551 mg/L EO/PO 0.017 0.017 Page 21 of 101
Sequoyah Nuclear Plant Biomonitoring December 4-11, 2007 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 22 of 101
BIOMONITORING CHAIN OF CUSTODY RECORD Page I of 1 W I 'Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Environmental Testing Solution, Inc. 351 Depot Street. Asheville, NC 28801 Phone: 828-350-9364 FedEx UPS Bus Client Other (specify): Sonic Delivery General Comments: I Delivered By (Circle One): Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Container Flow ,-7 Sample Description Number & MGD Rain Event? Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-Inches Number Temp. ance (°C) SQN-101-TOX Comp 12/02/07-12/03/07 0738-2 (2.5gal) NA X X 1__ 0638 i. I' SQN-INT-TOX Comp 12/02/07-12/03/07 0807-1 (2.5 gal) NA X X LnlzoI z.,4 0707 OH __Sample Custody - Fill In From Top Down , 4A., D JA Relinquished By (Signature): Date/Time Received By (Signature): Date/Time Chevy Williams / 12/03/07 Sonic Delivery 12/03/07 Sonic Delivery 12/03/07 ETS ,12/03/07 __A 5 q HM Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 35 1 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Container Flow
- I'*
Qot.3t55i Sample Description Number & MGD Rain Event? Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-Inches Number Temp. ance (°C) SQN-101-TOX Comp 12/04/07-12/05/07 .0752-2 (2.5gal) NA X X'l2c. (\\ '-s 0652 2z `!1 4) SQN-INT-TOX Comp 12/04/07-12/05/07 0824-1 (2.5 gal) NA X 0"412,32. 32S 0724 .3 Z.,- Sample Custody - Fill In From Top Down 4-C.uai Relinquished By (Signature): Date/Time Received By (signature): a 1/2 m'A Chevy Willi ams 12/05/07 -7 Sonic Delivery 12/05/07 Soi Delivery 1/0 12/5. Sonic. Delivery 12/05/07 ETS .12/05/07 d Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not anive until the following Monday.
L BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 lient: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 35 1 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Container Flow Sample Description Number & MGD Rain Event? Volume (Mark as Appropriate) Laboratory Use Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-Inches Number Temp. ance (°C) SQN-101-TOX
- Comp, 12/06/07-12/07/07
.0750-2 (2.5gal) NA X 0650 H,/o (3 SQN-INT-TOX Comp 12/06/07-12/07/07 0806-1 (2.5 gal) NA X 0.6 0706 -3 Sample Custody - Fill In From Top Down 4 Cu. 4-Relinquished By (Signature): Date/Time Received By (Signature):
- U Date/Time Chevy Williams 12/07/07
/ S Sonic Delivery120 Sonc/~` c-iv--/ So12/07/7iverT 12/07/07 Sonic.D Ielivery/ 12/0.7/07 ['-- ETS,, 12/07/07 1!.SC 4__ Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Environmental Testing Solutions, Inc. Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimeph ales promelas Client: TVA Facility: Sequoyah Nuclear Plant NPDES #: TN 0026450. Project #: 3bS!\\ Co'unty: Hamilton Treatment: Non-treated Outfall: 101 I Dilution pn Dilution prep (%) Test organism informationi: Test information: Organism age: 7-.0..1s Io A %3 OLb Randomizing template: q'-.IoI Date and times organisms k-O"*-al I 4oO0 Incubator number: were born between: zc_ Organism source: fýT'A &a-cr\\ 9e --ff*- cF Artemia lot number: Transfer bowl information: pH = Temperature 0C Total drying time: I+OmoAS "3-I "L "L~.. Date / Time in: I-L\\,-11. I Average transfer volume:. Date / Time out: .'-I ,..*L Oven temperature: Io" C... Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 -1 vqS t1250 i-L-o\\-' 4,, nI' pj'&.o0", ff-2 U 4 1.414S its_ Z \\Z-ot-0 -1 O 0-12i O 5. -U +23 3 '2.-(fl--i 61 Il___ IIt-01 -O011 61a0s7.,z7 4" -7 4 lz-ot-ui Oac3c IqO0 .OO oL -01 i 011?01. k2'. "t 6 17-o1-G o fsi IF\\ IL-oC-+1 alLa1l. a %L5 7£ Control information: Acceptance criteria Summary of test endpoints: % Mortality:
- 67.
-20% 7-day. LC50 )107. Average weight per initial larvae: 0.6.39 ..*W"K: NOEC tocs7 Average weight per surviving larvae: 0.65 3 & - 0.25 mg/larvae LOEC > 166 7. C ChV >ioo, IC j > /007. Page 26 of 101
Environmental Testing Solutions, Inc. Page 2 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: .L-C)-A-c\\, Survival and Growth Data Day CONTROL 11.3% 22.6% A B C D E I F G H I J K[ L I 0 /0 1 C0 /0 10 t0 /0 /10 Q 10 /C) 1C I /0 c /- / 0 I / o /0 /0 Ib /( 'o t 3'/ 0(c) (0/ 0 0C) / 0 /0(0 /01/0 5 -tO to to to 10 t 0 to 1 0 /0 '0 10 to 6 C0 /0 /0 1 0* '0 /0 /0 '0 1O 7 [0 1t /0 t! /0 10 10 /0 /0 /0 tO A Pan weight (mg) Color identification:'At.I.oY4 RA 0AR1-t S' 361I.I.9.S1 35t 11 CSiI-( Analyst: Lj-B = Pan + Larvae weight (rag) /., tZG l L Z --. 1 o,7.tZýj'
- z S
-Z&.L 26, U$ Z O. W 37,1 Z,4. Analyst: IM) Larvae weight (mg) = A-B (4 1?.o'4 -;**s.% o S-4 ,.11 11%'.4S 1-4* 0 1,21 ,ZI-O 7 (. l S,-\\ -1.31 I Weight per initial number of larvae (mg) P,\\ / '\\ *- A? C / Initial number of larvae c e 0* O O" 0" t t* t) b" O'* 0" Average Percent weight per reduction j7. initial from control .0..O2 number of (%) larvae (mg_) Comment codes: c = clear, d dead, fg fungus, k = killed, m = missing, sk sick, sm unusually small, lg = unusually large, d&r decanted and returned, w wounded. d r Calculations and data reviewed: Comments: Page 27 of 10f
Environmental Testing Solutions, Inc. Page 3 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: \\--o-C7 - Survival and Growth Data Day 45.2% 72.6% 100% S T 1 [O PR SU V XV x 0-D o b /0 1C).'0 /1 /0 /0 '0 /0 /0 S1 I0 I0 / (0 /0 /0 (0 (c0 /C C0 /O /1 2 1O ,ID / 0 / 0 /0 /0 /0 /0 /0 /0 3 /C I U t ' to (0 /0 (0 /0 /0 /0 to '0 /0 o /0 /0/o.. /0 /0 /C0 0 1 F3 10 10 /C) 'ý 0 10 Q) 0 1 c I~ C) 10 6 1 /!o 1r3(I u0 I0 '1 /t C1 C) Iu /0 7 %5 1c iz j-0 to (0 I0 0 10 10 /0 10 A Pan weight (mg) Color identification:At* W t... R**
- .O t.t qq
- ,l
- t.
q3 (0.4 5(0.% t3. Analyst: B = Pan + Larvae weight (mg) Analyst: MM z... 1S.t.., t9.%iO Z,.ii jq.o. zo. r", 1 ..6.2. Larvae weight (mg) A - B Weight per initial numbero'o ,$V Ž"' of larvae (mg) C / Initial number of larvae \\ 0 Average Percent weight per reduction initial from control -o. rsLo O5 number of (%) larvae (mg) Comment codes: c = clear, d dead, fg fungus, k = killed, m= missing, sk sick, sm = unusually small, Ic unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 28 of 101
Environmental Testing Solutions, Inc. Species: Pimephales promelas Page 4 of 6 Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: I )- - 0 1- Surrvival and Growth Data Day 100% Intake Y Z A BB ID oj'cb Ito 0 C 10 Q10 /J 2 10 /0/0 /o 3 1 /O / 1 c) 4 /0 IQ /0 D Q C) 10 10 / 6 /G3 /0 '0 /0 7 .k (0 A = Pan weight (mg) Color LtbtW lokt..k identification: 1 1{l3q.4 1i. DO Analyst: & ;G B = Pan + Larvae weight Analyst:,)/ Larvae weight (mg) A - B Weight per initial number of larvae (mg) C / Initial number of larvae .0 Average Percent weight per reduction initial from control number of (%) larvae (mg_) Comment codes: c = clear, d = dead, fg = fungus, k killed, m missing, sk sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w.= wounded. Calculations and data reviewed: Comments: Page 29 of 101
0 0 -5 0 TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11, 2007 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales prornelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 3551 Reveiwed by: Not for Cornpliarice Assessment, Internal Laboratory QC Co,,entration(%) Rtph-cet, ll05aenmb~rof FInsnalarettbroca, A-P.n-eight(mg) B-Pa..- L-s'.. L[-. -ewght (ng) Wighet/Sorv-A0nc M--1wdght/SevAinZg C-erertnt e*riaton W.Izht/Irsst sn.stsrs Mec-s.rA-iw Meanwtlhhl/Wthi1 Co~lltM, %I rof P-MrtC,dustdolfrus helttl (lm) -A-B numbre lar e. (nia) nsunmb,roltavc (se 5'tL.'5 p'rlOassO Loeo (ne,) (%) ,au ber e on I ronteet (%f A 10 t0 12.87 20.91 8.04 0.804 0.804 Control 13 10 10 14.89 20.24 5.35 0.5350638 20+0 0.535 0,638 20.0 Not Applicable C 10 10 14.88 20.28 5.40 0.540 0.0.540 100.0 D 10 10 15.96 2267 6.71 0.671 0.671 E 10 10 13.81 20.99 7.18 0.718 0.718 113 F 10 10 12.82 20.27 7.45 0.745 0,731 1.8 0.745 100.0 0.731 1.8 -14.7 G 10 10 15.09 22.49 7.40 0.740 0.740 H 10 10 15.13 22.35 7.22 0.722 0.722 1 10 10 13.56 20.65 7.09 0.709 0.709 22.6% J 10 10 13.77 20.28 6.51 0.651 0.703 12-0 0.651 97.5 0.682 7.2 -7.0 K 10 10 14.51 20.83 6.32 0.632 0.632 L 10 9 15.06 22.43 7.37 0.819 0.737 M 10 10 14.28 21.01 6.73 0.673 0.673 4, 10 10 14.39 20.35 5.96 0.596 0.619 8.6 0.596 0 10 10 12.70 18.26 5.56 0.556 0.556 P 10 10 13.34 19.86 6.52 0.652 0.652 10 10 14.42 20.99 6.57 0.657 0.657 72.6% R 10 10 12.74 19.02 6.28 0.628 0.639 2.2 0.628 100: 0.639 22 -0.2 S 10 10 14.08 20.35 6.27 0.627 0.627 T 10 10 14.22 20.65 6.43 0.643 0.643 U 10 10 1435 20.17 5.82 0.582 0.582 V 10 10 14.64 20.21 5.57 0.557 0.605 7.4 0.557 100 0.05 0.605_____ 7.45 100.0 0.60S 7.4 5.1 1 1o 10 14.56 21.14 6.58 0.658 0.658 X 10 10 13.98 20.22 624 0.624 0.624 Y 10 10 14.93 21.19 6.26 0.626 0.626 100% Intake Z 10 10 13.97 .20.96 699 0.699 0649 8.7 0.699 97.5 0.634 12.9 0.5 AA 10 9 13.85 19.07 J 5.22 0.580 0.522 Bit 10 o10 15.00 21.90 6.90 0.690 0.690 Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnet's MSD value' PN.S1: MSD = PMSD; 0.1076 16.9 0.1472 23.1 Minurum Significant Difference Percent Minimum Significant Difference PMSD is a re*sure of test precision. The PMSD is the minirorsmpercent difference between the control and treatmenit that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10th percentile) = 12%. Upper PMSD bound deterreined by USEPA (90th percentile) 30%. Lower and upper PMSD bounds were determined from the 10th arnd 901h percentile, respectively, of PMSD data flomEPA's WET hsterlaboratory Variability Study (USEPA, 2001a, USEPA, 2001b). USEPA 2001a, 2001b. Final Report. ltterlaboratory Variability Study ofEPA Short-term Chrooic and Acute Whole Effluent Toxicity Test Methods, Voluesses I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005 US Enivironmnental Protection Agency, Cincinnati, Ot
C TVA Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11, 2007 Statistical Analyses Larval Fish Growth and Survival Test-7 Day CGrowvth Start Date: 12/4/2007 Test ID: PpPFCR Sample lID: TVA./ Sequoyah Nuclear Plant, Outfall 1 01 End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.8040 0.5350 0.5400 0.6710 11.3 0.7180 0.7450 0.7400 0.7220 22.6 0.7090 0.6510 0.6320 0.7370 45.2 0.6730 0.5960 0.5560 0.6520 72.6 0.6570 . 0.6280 0.6270 0.6430 100 0.5820 .0.5570 0.6580 0.6240 Transform: Untransformed Rank I -Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 0.6375 1.0000 0.6375 0.5350 0.8040 20.018 4 0.6844 1.0000 11.3 0.7313 1.1471 0.7313 0.7180 0.7450 1.812 4 22.00 10.00 0.6844 1.0000 22.6 0.6823 1.0702 0.6823 0.6320 0.7370 7.188 4 20.00 10.00 0.6823 0.9969 45.2 0.6193 0.9714 0.6193 0.5560 0.6730 8.597 4 19.00 10.00 0.6290 0.9191 72.6 0.6388 1.0020 0.6388 0.6270 0.6570 2.223 4 18.00 .10.00 0.6290 0.9191 100 0:6053 0.9494 0.6053 0.5570 0.6580 7.391 4 18.00 10.00 0.6053 0.8844 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93847877 0.884 0.71715058 2.53040028 Bartlett's Test indicates unequal variances (p = 5.44E-03) 16.5491142 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 100 >100 1 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05 IC 10 IC15 1C20 IC25 IC40 IC50 36.221 87.670 >100 >100 >100 >100 >100 sqn_ 2-04-07data
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11, 2007 Statistical Analyses 0 Used for PN4SD calculation only. Larval Fish Growth and Survival Test-7 Day Growth Start Date: 12/4/2007 Test ID: PpPFCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCH-R-EPA-821 -R-02-013 Test Species; PP-Pimephales promelas Comments: Non-treated Cone-% 1 2 3 4 D-Control 0.8040 0.5350 0-5400 0.6710 11.3 0.7180 0.7450 0.7400 0.7220 22.6 0.7090 0.6510 0.6320 0.7370 45.2 0.6730 0.5960 . 0.5560 0.6520 72.6 0.6570 0.6280 0.6270 0.6430 100 0.5820 0.5570 0.6580 0.6240 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.6375 1.0000 0.6375 0.5350 .0.8040 20.018 4 11.3 0.7313 1.1471 0.7313 0.7180 0.7450 1.812 4 -2.101 2.410 0.1076 22.6 0.6823 1.0702 0.6823 0.6320 0.7370 7.188. 4 -1.003 2.410 0.1076 .45.2 0.6193 0.9714 0,6193 0.5560 0.6730 8.597 4 0.409 2.410 0.1076 72.6 0.6388 1.0020 0.6388 0.6270 0.6570 2.223 4 -0.028 2.410 0.1076 100 0.6053 0.9494 0.6053 0.5570 0.6580 7.391 4 0.723 2.410 0.1076 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93847877 0.884 0.71715058 2.53040028 Bartlett's Test indicates unequal variances (p = 5.44E-03) 16.5491142 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett'sTest 100 >100 1 0.10756027 0.16872199 0.00867097 0.00398382 0.10239999 5, 18 Treatments vs D-Control sqn_12-04-O7data
arc TVA / Sequoyah Nuclear Plant, Intake Non-treated December 04-11, 2007 Statistical Analyses Larval Fish Growth and Survival Test-7 Day CGrowth Start Date: 12/4/2007 Test ID: PpPFCR Sample ID: TVA I Sequoyah Nuclear Plant, Intake End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHl-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments:. Non-treated Conc-% 1 2 3 4 D-Control 0.8040 0.5350 0.5400 0.6710 100 0.6260 0.6990 0.5220 0.6900 Transform: Untransformed I-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6375 1.0000 0.6375 0.5350 0.8040 20.018 4 0.6375 1.0000 100 0.6343 0.9949 0.6343 0.5220 0.6990 12.863 4 0.043 1.943 0.1472 0.6343 0.9949 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.91297191 0.749 0.32811175 -0.7982733 F-Test indicates equal variances (p = 0.48) 2.44667292 47.4672279 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.14716277 0.23084356 2.1125E-05 0.01147096 0.96716261 1,6 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05 IC10 IC15 IC20 IC25 IC40 IC50 >100 >100 >100 >100 >100 >100 >100 sqn_] 2-04-07data
0 0 TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11, 2007 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Pimephales promelas Daily Chemical Analyses Project number: 3551 Reviewed by: Concentration Parameter Dav 0 Da I Da 2 Day 3 IIIaIDay 4 Day 5 Ina Day 6 pH (SU) 7.83 7.56 7.381 7.59 7.83 7.75 8.061 7.67 8.10 7.71 8.15 7.921 8.021 8.0-DO (mg/L) 7.7 7.5 7.7 8.0 7.6 7.6 7.7 6.8 7.6 7.27.6 7.9 7.4 Conductivity (jimhos/cm) 296 310 337 304317 Control Alkalinity (mg/L CaCO 3) 57 57 57 Hardness (mg/L CaCO 3) 92 94 92 Temperature (TC) 24.7 24.5 24.6 24.3 24.6 24.6 24.7 24.3 24.6 24.7 24.8 24.3 24.7 24.: pH (SU) 7.58 7.53 7.57 7.57 7.67 8.00 7.67 8.04 7.71 8.09 7.92 8.02 8.01 11.3% DO (mg/L) 8.0 7.5 7.6 7.9 7.9 7.7 7.4 6.9 8.0 7.2 7.8 7.9 8.2 7._ Conductivity (t~mhos/cm) 287 315 312 318 293 296 302 Temperature (CC) 24.7 24.5 24.6 24.5 24.7 24.3 24.7 24.5 24.6 24.5 24.8 24.2 24.7 24.! pH (SU) 7.58 7.53 7.56 7.54 7.69 7.81 8.04 7.67 8.05 7.71 8.09 7.93 8.05 8.0( DO (mg/L) 8.0 7.6 7.6 7.9 7.9 7.6 7.6 6.9 7.9 7.1 7.8 7.8 7.91 7.( 22'6/ Conductivity (itmhos/cm) 280 305
- 304, 320 285 288 3001 Temperature ('C) 24.8 24.5 24.6 24.6 24.7 24.6 24.7 24.5 24.6 24.5 24.8 24.5 24.9 24.
pH (SU) 7.58 7.52 7.56 7.56 7.70 7.86 8.07 7.69 8.05 7.72 8.09 7.96 8.07 8.01 45.2% DO (mg/L) 8.1 7.6 7.6 7.8 8.0 7.6 7.6 6.9 7.9 7.0 8.0 7.8 8.0 7.( Conductivity (ýtmhos/cm) " 263 287 281 307 267 255 280 _Temperature ('C) 24.8 24.5 24.7 24.6 24.7 24.4 24.7 24.6 24.7 24.5 24.9 24.1 24.9 24.( PH (SU) 7.54 7.53 7.57 7.58 7.71 7.86 8.08 7.72 8.04 7.79 8.13 8.05 8.09 8.1( DO (mg/L) 8.1 7.6 7.7 7.8 8.2 T7.8 7.6 6.9 8.0 7.1 8.0 7.8 8.2 7.: Conductivity @tmhos/cm) 241 264 260 268 246 249 248 Temperature ('C), 24.9 . 24.6 24.7 24.5 24.8 24.4 24.8 24.3 24.7 24.4 24.9 24.5 25.0 24.: pH (SU) 7.51 7.56 7.57 7.63 7.74 7.85 8.11 7.78 8.03 7.78 8.14 8.06 8.141 8.1: DO (myg/L) 8.2 7.6 7.7 . 7.6 8.2 7.9 7.7 6.9 8.1 7.0 8.1 7.9 8.11 7.: Conductivity (pLmhoslcm) 220 241 237 250 225229235 100% Alkalinity (mg/L CaCO3) 68 72M76 Hardness (mg/L CaCO 3) 86 78 98 Total Residual Chlorine (mg/L) <0.*1 <0.10 <0.10 Temperature ('C) 25.0 24.7 24.8 .. 24.9 24.3 24.8 24.6 24.7 24.4 24.8 24.4 25.0 24. pH (SU) 7.481 7.58 7.61 7.63 7.75 7.86 8.09 7.79 7.95 7.76 .8.09 8.03 8.13 .. 0 DO (.g/L) 8.41 7.6 7.71 7.7 8.1 7.91 .77 8.11 6.9 8.2 7.7 8.1 7. Conductivity (ttmhos/cm) 220 251 237 249 222-23 100% Intake Alkalinity (mg/L CaCO3) 70 74 Hardness (mg/L CaCO1) 8 88 94 Total Residual Chlorine (mg/I) <0.10 <0.10 <0.10 T'emperature ('C) 25.01 24.51 24.8 24.7 24.9 24.5 24.8 24.4 24.71 2 24.9 24.4 24.9 24.
Environmental Testing Solutions, Inc. P a g, f 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated L'U;hY I'l ,Iqtv Date: k I-CA-C7', Day 1" T 2 0 I 2 Analyst i, L1YL-Concentration Parameter CONTROL _pH ('41) DO (mr2/L) (ýimh scm)______ 1A .;1 Alkalinity (mg CaCO 3/L). Si i Hardness Temperatur'e (C) -LA - 1 tq 'S tq 1.0__ __q__ ZIA to PH (S.U.) ?__ 7_ __
- 4 11.3%
DO (mg/L) u_ ConductivityOva (_Tmhos/cm) .L 1_.. '4 Tem._=_.perat u re ('C) %4.,-S LA* So -Lg -4,1"L IAII (S.U.) 9.T3)Z 2 ~yL) C) CJ !:k,,M.*( -140 22.6% DO (mqiL) ',)9 ,q - 57 Conductivity IL (1imhos/m 041 A-- .AY Temperature ('C) Aq-1` 14.6 9 9 Ii 9 9 ~ - pH (S.U.) _9. f57~ ýU.,.0 45.2% DO (mgrL) q i ,7)I_ Conductivity q (l4mhos/cm)
- 4.
T______ !emperature ('C) 'LA ? -4 13 LMI. 4 Z.I/-,A I LAA pH (S.U.) i§- q,0 Xl)5 j o 72.6% DO (mgiL) (.. Conductivity ,2-
- -l(rmhos/cm)
Temperature ('C) 114.*TC 1 V. 4 _L4 P*H (s.u.) -1.-15 100% DO (mg/L) Conductivity (*.mhos/cm) Alkalinity (mg CaCO 3/L) Hardness (mg CaCO3/L) TR chlorine 0, 0 (mg/L) Temperature(0 C) (.Q0 N -. 1. z. 2, pH (S. U.) '.,1 (P "7.).5 .U I -. O._5 ')..),y 100% Intake DO (mg/L) 4:,,14 Conductivity (iimhos/cm) 9L5 Alkalinity (ing CaCO 3/L) Hardness (mg CaCo-/L) TR chlorine (Mg/L) Temperature (OC) 2Sc.0 Z "q.. .q. 2. q." Page 35 of 101 Initial .11 Final I I Initial 11 Final
Environmental Testing Solutions, Inc. Page 6 of'6 Species: Pinmephales promelas Date: _ Client: TVA / Sequoyah Nuclear Plant - Non-treated D ay 3 4 5 5 6 AnlI Concen-Parameter M, % l '014 CONTROL p(s.u.) F. ( U.).U .ro " 9- & o DO L(ngL) ). Conductivity (ýmhos/cmn) .3i" L&2L Alkalinity (Mg CaCO 3/L") (mg aCO3/L) .4 A Temperature(°C) ('Q ")..q 1 ".q- .A.\\ PH (S.U.) , -L I 'Vq, k
- i.
o-A. 11.3% DO.(m) L) Conductivity (,Lmhos/cm) Temperature ('C) -LA -S ". L-S 7A VA- -L 22.6% DO (rn L)- j*r Conductivity 20 Conductivity (Vmhos/cm) LJT}. Ternperature ('0 Q I-A1 -LA.c, -1452'. ~
- i.
.~I PH(mg CaC.) s 72.6% DO (m a/L) , "Tern)erature (°C) -I' 2q, V.I. A- $i _2.62q~ ".$0 q." Conductivity (ý hos/co) .Z TRchlorine (m /L) Z.O. Temperature('C) MI2.".7 A-(o Z S 100% DOI(mgiL) F. Fain Hardness (mg CaCOfL) TR Chlorine (mg/L) zo Ternperature ('C) 2 1q44 74A124
- q.
nt a PHiia Fia Iiia inl IitaFnl ia Page 36 of 101
Environmefntal Testing Solutions, Inc. Page I of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) .Species: Pimeph ales. promelas Client: TVA Facility: Sequoyah Nuclear Plant NPDES 9: TN 0026450 Project#: 3SS\\ County: Hamilton Treatment: UV-treated Outfall: 101 Dilution reparation information: Comments: Diluto-prep (%) 11.3 22.6 45.2 72.6 100 Each concentration was treated Etfluent volume (mL) 282.5 339 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2217.5 21.61 1370 685 0 to remove pathogenic Total volume (mL) 2500: 2500 2500 2500 2500 interferences. Test organism information: Test information: Organism age: 2Db. O*kS 5 0*C,_ Randomizing template: gLwe Date and times organisms I*OO Incubator number: were born between: Organism source: -iPr Sf ýxL-Oý.-0210 Artemia lot number: I*O-ZCA-j Transfer bowl information: pH" Temperature = 'C Total drying time: 7.,,Aao-% 1-k -L-Date / Time in: .1 jmL5O Average transfer volume: Date / Time out: rL4tL.0, _'_ISO Oven temperature: Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, MHS Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 0 \\--- A t0a ,o1-0 01 2 o j--- t4 o
- q %LA k :
I v 5 0- 0 1- 0n 6 C-I 1 2 0S.7.Z i -z s 2 3 k-' 0 1qoL wl -01" 0 k O"! 7- -1 L-5 6 IlI 061 OcYt 154 tko0q tiL' t-1 \\ -- (A
- 1*
l ai L4I, r Control information: Acceptance criteria Summary of test endpoints: % Mortality: < 20% 7-day LC5 0 > 11O7. Average weight per initial larvae:
- o. "1 t NOEC I OQ Average weight per surviving larvae:
0.1 !,A- > 0.25 mg/larvae LOEC "> I Y ChV > tOO7 I> t 07, Page 37 of 101
Environmental Testing Solutions, Inc, Page 2 of 6 Species: Pimeph ales prom elas Client: TVA / Sequoyah Nuclear Plant - LW'-treated Date: t%-C"" Survival and Growth Data Day CO.NTROL 11.3% 22.6% A B C D E F G 1H1 l31 K L 0 J 0 ) 1C t (0 io t0 /0 /0 / D lb /C /C Q1 1o /0 /0 /0 to '0 lb /0 '0 00 tO 2 t0 10 1C0 1I .D ID I0 Db I0 lo to Il /0 10 /0 /0 /0 / I C) /C)0 '0 CO 4 /Q t(o to to ,0
- 0.
/0 I I0 /C /0 /0 1 / b0 IC- /o to /0 0 to /I to to 6 1 10 o0 I' 4 C0 C) /0 / 3 ( ) /0) 7 tt.*. t** L f IC 00 9 10 t ( tC f0 / 0 O0 A Pan weight (mg) Color identification: C, to t t3.00 t4,S'ý N.1% 13.84 t3-13 kj.t$ t', 1q.3-1 I q.7$ q.C 1.* 5 5 4 a Analyst: LA4( B = Pan + Larvae weight (mg) Analyst: L i*.* 19ý.% i.9 a,3O.5, I¶---aa q at.51 aa,eS ai.3 ,o.61 at.g Larvae weight (mg) =A -B Weight per initial number of larvae (mg) = C / Initial number of larvae 0' \\ Q0 0" '0 t* Average Percent weight per reduction initial from control 0.1 number of larvae (mg) 0.1.7 Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, Ig unusually large,.d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 38 of 101
Environmental Testing Solutions, Inc. Pace 1 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: \\.-cA'\\ Survival and Growth Data Day 45.2% 72.6% 100% Q Ro' S U V x 0 ~__ [0 O li O t /0 /0 (. C /y /_JO /0 /0 to 00 /0 /0 /. /c !0 /U /tc /0 /© 21 10 1CO it to IO U O I o CO I0 /O t .2) .10 tO 10 10 lb 10 -0 /0 /0 /0 /0 31 10 (10 0o to l() C) /0 /o /0) /D 4 0 to /0 /0 /0 /0 /0 'C> /0 'o '0 /C) /U /I /c 10 /0 lo to '0 /0 /0 /o /0 )6 (0 0 10 I1 to (0 t0 t( /0 io io /0 7 it to (C tO (I t(U 0 1 C 0 C) t C) '/ -it A = Pan weight (mg) Color identification: (40_rk\\~ t1.. OVA% K- .*P& N-04 IS-.11 1,'1-9 q~0 t
- ,8 A00* IS'-*
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q.,t* Analyst: B = Pan + Larvae weight Analyst: z.3 .1a -sa.P0 w a Larvae weight (mg) = A - B 16*j t,.SR' 5.101 66 o IAl lo,
- 1.
- 6.
(o.S4 O 6 '.01 2.,7 (e.'4 Weight per initial number of larvae (mg) e C / Initial number of larvae b" O" O" c" e," O" O t' Average Peicent weight per reduction initial from control
- 0. e 4.1 &Z 1 O'
.JtSO number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m m issing, sk A sick, smn unusually small, ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 39 of 11U
Environmental Testing Solutions, Inc.. Page 4 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: r?"- A-(r-Survival and Growth Data Day 100% Intake Y Z AA BB ') 0 ~0
- t 0
2 4 to (00 /0 t 0 10O 10 tO (0 tO (0 10 A Pan weight (mg) Color identification: Analyst: L" B = Pan + Larvae weight (mg) A.13 to ?a .,xOqt at.ll. Analyst: Larvae weight (mg) = A-B Weight per initial number of larvae (mg) A C / Initial number of larvae 0*- Average Percent weight per reduction initial from control
- 0. 10l number of
(%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, go= unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: I Page 40 of 10
C 0 TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated December 04-11, 2007 / Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Reveiwed by: fe *" trt /L 44-4.--- Project number: 3551 r Not for Complia nce Assessment, Internal Laboratory QC Concentration (% ) Replicate Initial ou mber of Final number of A - Pan weight B =Pan,+ Larvae Larvae weight (rog) W eight uI clo. a ght. C W eightloitiel no otb Mean urvi M ean weightI Coeficdienr of Percent rea*ctionl Icrn larv a e lI a
- r.
( igt) w eig h t ( ag ) A B n tb er of larv ae (cog) S u ro lv io g n u m b er of (t11 - .dah, In, *.0
- 1.,
o florv a (tm g) (0 4 ) I cilta l n u n b er of v a riatio n c-*., hltc co n tro l (% ) larva e (n.g I ,netr *tt n2(5) mo rv ".-I r.Itdotneef..... l -) A t0 0 13.00 19-82 6.82 0.682 0.682 Control 1 [0 9 14.39 19.95 5.56 0.618 0.734 13.8 0.556 97.5 0.719 17.6 Not applicable C 10 t0 14.78 23.08 8.30 0.830 0.830 1 10 10 13.84 21.92 8.08 0.808 0.808 E 10 9 13.43 19.52 6.09 0.677 0.609 11.3' F 10 10 13.38 20.02 6.64 0.664 0.688 3.8 0.664 97.5 0.671 7.2 6.7 G 10 10 14.37 21.24 6.87 0.687 0.687 t to 10 14.33 21.57 . 7.24 0.724 0.724 t 10 10 14.50 22.5 1 8.0 1 0,801 0.80 I 226J 10 10 14.72 21.33 6.61 0.661 0.740 8.1 0.661 100.0 0.740 8.1 -2.9 K 1 0 K0 1 3.5 7 12 0.8 7 7.3 0 0.7 3 0 0.7 3 0 L 10 10 14.21 21.88 7.67 0.767 0.767 M 10 10 14.48 21.64 7.16 0.716 0.716 45.2% N 10 10 14.66 21.25 6.59 0.659 0.685 (0.5 0.659 100.0 0.685 4.7 0 10 10 14.81 20.80 5.99 0.599 0.599 P 10 10 14.04 21.70 7.66 0.766 0.766 Q 10 10 13.97 20.89 6.92 0.692 0.692 7 2.6 % R 1 0 I0 1 3.9 0 2 0.7 8 6.8 8 0.6 8 8 0.0 3 8.7 0.6 8 8 _ _ _ _ _ _ _07 9100.0 0.703 8.7 2.2 S 10 10 14.60 22.49 7.89 0.789 0.789 T to 10 13.88 20.31 6.43 ý0.643 0.643-10%V 10 10 (4.42 20.45 6.03 0.603 0.650 6.3 0.603 100.0 0.680 6.3 9.7 U 10 10 12.61 19.11 6.50 0.650 0.650 W (0 t0 14.58 21.60. 7.02 0.702 -0.702 X 10 (0 14.16 20.59 6.43 0.643 0.643 1 (0 (0 15.85 22.76 6.91 0.691 0.691 180%y inttake Z (0 (0 14.81 21.92 7.11 0.711 0.709 2.8 0.7(11 o .0 A A to 10 13.60 20.96 7.36 0!736 0.736 I B B I 1t 10 14.99 1 2 1. _97 6.98 0.698 1 0.698111 Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnetl's MSD value: PMSD: MSD = Minimum Significant Difference 0.1256 17.5 0.1246 17.3 PMSD= Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the mninimumr percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMsD bound dleterarmned by USEPA (I0tit percentile) - 12%. Upper PMSD bound determined by USEPA (90th percentile) = 30%. Lower and upper PMSD bounds were dletenmined from the 10th and 90th percentile, respectively, of PMSD data fi'om EPA's WET Interlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001[b). USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic andAcute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appencdix. EPA-82I-B-01-004 and EPA-82 I-B-01.005. US Environmental Protection Agency, Cincinnati, ON.
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated 0 December 04-11, 2007 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 12/4/2007 Test ID: PpPFCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.6820 0.5560 0.8300 0.8080 11.3 0.6090 0.6640 0.6870 0.7240 22.6 0.8010 0.6610 0.7300 0.7670 45.2 0.7160 0.6590 0.5990 0.7660 72.6 0.6920 0.6880 0.7890 0.6430 100 0.6500 0.6030 0.7020 0.6430 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat. Critical MSD Mean N-Mean D-Control 0.7190 1.0000 0.7190 0.5560 0.8300 17.626 4 0.7190 1.0000 11.3 0.6710 0.9332 0.6710 0.6090 0.7240 7.177 4 0.921 2.410 0.1256 0.7054 0.9811 22.6 0.7398 1.0289 0.7398 0.6610 0.8010 8.107 4 -0.398 2.410 0.1256 0.7054 0.9811 45.2 0.6850 0.9527 0.6850 0.5990 0.7660 10.525 4 0.652 2.410 0.1256 0.6940 0.9652 72.6 0.7030 0.9777 0.7030 0.6430 0.7890 8.746. 4 0.307 2.410 0.1256 0.6940 0.9652 .100 0.6495 0.9033 0.6495 0.6030 0.7020 6.261 4 1.333 2.410 0.1256 0.6495 0.9033 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97521883 0.884 -0.3876268 0.23957661 Bartlett's Test indicates equal variances (p = 0.45) 4.72200441 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.12562972 0.1747284 0.00431004 0.00543476 0.56852084 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05 79.342 ICtO IC15 IC20 IC25 1C40 IC50 >100 >100 >100 >100 >100 >100 sqn_05-30-O7data
4ý1 Co 0. TVA I Sequoyah Nuclear Plant, Intake UV-treated December 04-11, 2007 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 12/4/2007 Test ID: PpPFCR Sample ID: TVA / Sequoyah Nuclear Plant, Intake End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.6820 0.5560 0.8300 0.8080 100 0.6910 0.7110 0.7360 0.6980 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical* MSD Mean N-Mean D-Control 0.7190 1.0000 0.7190 0.5560 0.8300 17.626 4 0.7190 1.0000 100 0.7090 0.9861 0.7090 0.6910 0.7360 2.795 4 0.156 1.943 0.1246 0.7090 0.9861 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93012404 0.749 -0.7387113 1.46486826 F-Test indicates equal variances (p = 0.01) 40.8998299 47.4672279 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.12462388 0.17332945 0.0002 0.00822633 0.88120699 1, 6 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05 >100 IC10 IC15 IC20 IC25 IC40 IC50 >100 >100 >100 >100 >100 >100 sqnO5-30-O7data
4ý- 4C 0 TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated December 04-11, 2007 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promelas Daily Chemical Analyses Project number: 3551 Reviewed b Concentration Parameter Day 0 Da 1 Day 2 Da 3 Day 4 Day 5 Da 6 Initial Final Initial I Final Initial I Final Initial Final Initial I Final Initial Final Initial Final PH (SU) 7.62 7.55 7.611 7.58 7.671 7.86 7.99 7.68 8.021 7.71 8.08 7.92 8.01 7,98 DO (mg/L) 8.0 7.7 .7.71 7.9 7.91 7.7 7.6 7.2 7.71 7.1 7.91 7.8 8.01 7.6 Conductivity (4mhos/cm) 295 .3316 31* 333 294I 306 33 Control Alkalinity (mg/L CaCO 3) 57I58 57 Hardness (mg/L CaC0 3) 88 92 88 Temperature (°C) 24.8 24.7 24.7 24.6 24.8-24.6 24.6 24.6 24.7 247 . 24.6 24.3 24.8 24.4 PH (SU) 7.61 753 7.61 7.57 7.68 7.80 8.04 7.63 8.01 7.69 8.08 7.89 8.05 7.97 DO (mg/L) 7.9 7.9 7.7 7.9 .7.8 7.7 7.7 7.2 7.7[ 7.0 7.9 7.7 7.9 76 Conductivity (1imhos/cm) 288 319 315 329 t 295 i 301 319 Temperature (°C) 24.9 24.5 24.8 24.4 ..24.8 24.2 24.8 24.3. 24.7 24.5 24.8 24.3 24.9 24.6 pH (SU1) 7.60 7.53 7.61 7.58 7.61 7.85 8.05 7.62 7.99 7.79 8.10 7.95 8.06 8.00 DO (mgIL) 7.9 7.7 7.7 7.9 7.81 7.6 7.7 6.8 7.71 6.9 7.91 7.6, 7.9 76 22.6%/o Conductivity (,umhos/cm) 281 308 .3050 311 277 293 291 Temperature (°C) 24.9 24.9 24.4 24.91 24.2 24.8. 24.4 24.7 24.6 24.8 24.1 25.0 24.6 pH (S U) 755 7.61 7.59 7.63 7.85 8.08 7.62 7.99 7.78. 8.12 7.99 .8.09 . 8.03 DO (rg/L) 7.9 7.6 8.0 7.9 7.6 7.8 6.6 7.8 7.1 7.9 7.5 7.9 7.7 45.2% Conductivity (pImhos/cm) 259 294 286 3o 305 260 275 285 Temperature (°C) 24.9 24.8 24.9 24.7 25.0 24.3 24.9 24.2 24.7 24..8 24.5 25.0 24.3 pH (SU) 7.55 7.55 7.61 7.56 7.62 7.90 8.09 7.83 7.99 7.82 8.15 8.02 8.12 8.05 DO (mg/L). 8.0 7.7 7.8 8.0 8.1
- 7.7 7.8 6.6 7.9 7.0
- 81)
.7.6 7.8 7.7 72.60/a Conductivity (jimhos/cm) 236 266 261 278 239 253 258 Temperature ('C) 25.0 24.8 24,9 24.6 25.0 24.3 24.9 24.1 24.7 24.2 24.9 24.2 25.1 24.5 pH (SU) 7.52 7.61 7.62 7.57 7.67 7.90 8.12, 7.76. 8.001 7.821 8.18, 8.061 8.&15 8.09 DO (mg/L) 8.1 7 7.8 8.0 8.1 7.8 7.9 6.81 8.21 7.1
- 8.
7.6[. 7.8 7.7 Conductivity (limhos/cm) 219 245 241 220 232 237 1000/a Alkalinity (mg/L CaCO 3) 72 661 70 Hardness (mg/L CaCO 3) 90 94 86 Temperature ('C) 25.1 .246 25,0 25.11 24.21 24.9 24.1 1. 24.8 24.5 24.9 24.25 24.5 pH (SU) 7.521 759 7.64 7.60 7.65 7.861 8.12 7.771 7.981 7.85 8.131 8.04 8.16 8.08 DO (mg/L) 8.3 7.8 7.71 8.21 8.16 7.81 9 6.9 8.3 7.1 8.2 7.7 7.8 7.7 Conductivity (ý.smhos/crn) 217 27237 237252326 100% Intake Alkalinity. (mg/L CaCO 3) -6 70 Hardness (mg/L CaCO])
- 8 889 Temperature (0C) I 25.0 24.6 24.8 24.41 25.01 24.6 249 24.2 24.8 24.2 24.9 24.2 24.8 24.6
I Environmental Testing Solutions, Inc. Page 5 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant-UV-treated Date: i.- CA - G') DailyC I Day I i = I 0 I 1 I 2 I 9 II II Analyst I P' Concentration I Parameter CONTROL PH (S.U.) DO (mg/L) Conductivity (urmhos/cm) ,,{ Temerature ("C) I 4-pH (S.U.) i-.S l1.W 'A
- I zA't.
za~q II-L,*.S IN:,* I 7.,t~ I LA - II L -t 11.3% DO (mg/L) Conductivity (jmhos/cm) Temperature ('C) pH (S.U.) 22.6% DO (mgIL) ( Conductivity Temperature (°C) TA.9 .iA- "'-L-- pH (S.U.) I O.Z3 II7* 9tl 5s II 45.2% DO (mg/L) 7C Conductivity 15q (prmhos/cm) Temperature ('QC) ~ 2i.Z 2.9'. 1 pH.(S.U.) .) 1 -0 ,/ =(0~ ).C 72.6% DO (mgrL) 13._ Conductivity (prnhos/cm) _______Temperature (C) 150 7qj. 1.. .0. ApH (.,. Z), I ,II 100% k- ) Conductivity (pmrhos/cm) IAr I%~LI A 4 Temperature ('C) iSA fl PH (S.U.) t 100% Intake jDO (mgfL) Conductivity (jimhos/cm) I Temperature (°C) ZS. 0 "*..3 01 1 14, 11o I1 -a S
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2zq. f IN-Lq "I,* Initial 11 aI L Initial 11 Final I Initial II Final I 0 C)a A1 r" IC: C Page 45 of 101 C,7a -.7
I Environmental Testing Solutions, Inc. Page 6 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: \\'A--cr-Dav 4 5 6 Anal st P Concen-Parameter* tration CONTROL-----.--' 1... e.op a DO (mg/L) Conductivity 13 0 (ýi mos/m) Temperature ('C) te,-2.0- "/'
- :,..*-.a PH (S.U.)
r~q-70 11.3% DO (mg[L) Conductivity &tmhos/cm) Temperature(°C).Q "A., b,- 1 Z L a,. .(, pH (S.U.) ( (.0 22.6% DO (mgfL) Conductivity O(PM oS/cM) Temperature(°C) (_Q 114 -1 152 45.2% DO (gL) Conductivity l L (4mrhos/cm). 0 L. Temperature (°C) 14.9F. O_ pH (S.U.) el7 72.6% DO (mZ 0 Conductivity25 Ternperature ('C) 24 A ~*.. 4 100% DO(mg[L) 14-(4 -ills Conductivity (mhos/cm) C) 930*.
- Temperature ('C)
-L.* "lL1-
- t L5 100%
DO (.r..L) Intake ConductivityUSkc (4mhos/cm) ______.1 Temperature (0 C 4'Q2.15 ~i. ~ 1 Initial U Final I Initial If Innat i al n I
- flrLa, Initial II Final I
rage 46 of 1 01~ ~JZ. hX\\14. ~L.. 7cz
Environmental Testingo Solutions, Inc. Paýýe I of.7 Environmental Testing Solutions, Inc. Page 1 of? Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA Facility: Sequovah Nuclear Plant NPDES #: TN 0026450 Project#: ms*-z;S County: Hamilton Treatment: Non-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 339 1130 1815 2500 Diluent volume (mL) 2217.5 2161 1370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information: Organism age: 4 VA-A oQ.VLG o,..t) Randomizing template: 5)A,0 Date and times organisms 'L.-oa.-"L1 01'wL. -To -oO .Incubator number and 2..7-were born between: shelf location: Organism Source Cups: YCT batch: Culture board: W-1---1 A 61.*46*,4,6o 12 1'%1 Ito, 1-Transfer bowl information: pH =".&E Temperature = -8. R Selenastrum batch: i\\--Cfl Daily renewal information: Day Date Test initiation, Control water Sample numbers Analyst renewal, or batch used used termination time fl-ds-cr I 00(: Vz-O*-*A, cnz's. rsog 4 3 tz*--' OO'L_ !.-- t~n~ (' oaz*'_t24Z.' Control information: 1 2 Acceptance criteria Summary of test end oints: %ofMaleAdults:
- 7.
- 20%
7-day LCQ0 .1I%' % Adults having 3rd Broods: bc 7, oo07. >80% NOEC I w7__. % Mortality:
- 67.
0 _ 20% LOEC lz1r575 Mean OffspringfFemale: 33%,.%. _ 15.0 offspring/female ChV > UO7 % CV: -1. 177. 6."17. < < 40.0 % 1C25 ) 1007. Page 47 of 101
Environmental Testing Solutions, Inc. Page 2 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: CONTROL - I Survival and Reproduction Data Replicate number Dav 1 2 3 4 5 6 7 8 9 10 S]Youngproduced ©1e1 \\l i ] iJ* E___tm:- I-- t___ a) I C_ Adul mo tlt 1 - - i I _ _ ] _ I [.___ Young produced o C) C c* ! dult mortality 1 l-" 6 Young produced C c C ) Adult mortality __ -1I-!*i t--I--I\\--I*-- 4 Young produced LI A A I 'I A I %A A Total young produced c_ Final Adult mortality L.' s -. I kI Xfor un prood uced c j 1 0 -I) C1 - Note: Adult mortality (L live, D e)_Concentration: 7 on~rdcdI Conentration:- IL D ý a% ) 2 3 A -S o6 9 1 % Mortality: o7. Mean Offspring /Female: 3ý.0% CONC: 11.3% Survival and Reproduction Data Da_____ __Replicate number _ Day____ 1 213 4 S] 6 7 8 9 10 1 Youngproduced I 0 c 0 C 0 C 0 0 2 Young produced ~..___ Adult mortality _.'L___ L
- L---
L-s- Young produced C) 0 I T I 0 0 ]Adultmortality t U[ \\K- \\. I _I_ I \\--- 4 Young produced %A LA '© VWI4 Adult mortality I L{ \\ -] '--_ 5 Young produced [2I %A I 'L2 3 13 1 L I 12* 2_ I IAdult mortality I l '- ' -- IV---I L I L --t. 1L._I U_.. ] 61 Adult mortality____ 6 Youngproduced ] ) C)O
- 0) o
) ) o [ Adult mortality [t. . I L I - 7 ý Young produced is 19 1 -1 0 tIko I,,o CiI' s l -1 Total young produced 3 3 3 -"I 3 I 3 -1 3 331,3 Z 31 52 Final Adult Mortalit L KI. ] k J -., -I Zi. L Note: Adult mortality (L = live, D dead) Concentration: % Mortality: Mean Offspring/Female: 33.S I I I Page 48 of 101 I% Reduction from Control:I - S.0 Environmental T'esting Solutions, Inc. Pag-e. " of 7 I I I I I I I I Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: coNc: 22.6% Survival and Reproduction Data Replicate number Day 1 2 _3 j _
- 62.
7 8 9 6o i ]Z 1 u2grdue O I'O O b I- ©___ O I e dult mortality .1 j_.. 7__ Y oung produced 7 ) S C* SAdult mortal,,t,, [ --J
- IL- -.- J _L.- I L--
L__-_ Young produced Adult mortality i t t °L A d u l I- ý - 1 oe Adult mo rtality (,lie D.dead) YounConcncedrAtin Adult mortality*' TtlYoung produced a N t: Adult mortality ( ie .ed % Mortality:
- 07. 1 TtMean Offspring/Female y3-. &
% Reduction from Control: .17. CONC: 45.2% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 Y Young produced C 0 01 0 0 0 0 Adult mortality 2 ::** Yougtrodce 1 1_o o_ 01 I10 Iof ° Ic 3 Young produced c) j 0] __I 1Adult mortality [ L I 1--- IL1 I 5 Young produced 1 CT) Adult mty J U J \\-jt... 7 Young produced.1,1 Tf 7 ZO 1 8 1
- 7.
l, Totalyoung produced rS
- 3.
3.__
- 35.
- 3..-
I o0 Final Adult Mortality C ~ C ~ ~ Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: )7. Mean Offspring/Female: 3,., % Reduction from Control: I. 7. I Page 49 of 101
Environmental Testing Solutions, Inc. Page 4 of 7 Date: _-A-c-\\ Species: Ceriodaphnia dubia Client: Suequovah Nuclear Plant - Non-treated crypt 72.6% Survival and Reproduction Data Replicate number Day 2 3 [ 4 5 6 7 8 9 10 Y Voungproduced -) 7c 0 Adult mortality Young produced 0 0 CQ)j______ Adult mnortality k. ._.I_. k _. Adult mortality iL j II Young produced IL I 01 i I t*Tc' L-3i, J Adult mortality I_._. I__ I I. v Ii_. k _-\\ 6[Young produced 1ILA0+/-AC.(i 3)3I$ Adult mortality_, L f J 7 Young produce d o iJL z C) 2 I I Total young produced 3% -56 '4 0 s N 0 a Final Adult N-ortality Vk7\\. I L ~ L Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: C7. Mean Offspring/Female: S11. I % Reduction from Control: CONC: 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 2 jYoun produced 1/21 C 2 C1 0313 ]& I 10 " Adult mortality I \\_., {
- k.
L... LI 4 Young produced Adult mortality Adult mortality fik..._1_...
- I..-
13 Ji___ 6 Young produced [IA "-Af~i I [ 0 1 t unp duAdult mortality [ 7._ L _ I [. Y Young produced fl 16 is"* 1Si" is Total young produced ,3 p p3, c'- i 31
- 1 Final Adult Mortalit y
U Note: Adult mortality (L = live, D dead) Concentration: " u% Mortality: m Mean Offspring/Female:
- 2.
I [Reduction from Control: - 20.,, IPage 50 of 101
Environmental Testing Solutions, Inc. Page 5 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: I, -L o-OLA-c CONTROL -2 Survival and Reproduction Data Replicate number Da 2 3 1 4 5 6 1 7 8 9 10 Young produced-01- _ 1* 2 Young produced Adult mortality 2 Young produced c) aTCo_.c-Note: Adult mortality __e D de ) Cnnproduced q 4tati ldult mortality J0. eYoungproduced fnm IL \\t7 o Youngproduced Cr C i ad C5 =bi Dat Adult mortality \\,- Ib Damrtlyt L 2 3- _5 6_ 7_ _9 7 Young produced IS 31 1 iS I L -1 15 1 to Total young produced 30 3o -0 3~a 2ý a63 3~ 31 a Final Adult Mortality t.. L \\' Note: Adult mortality (L live, D dead)--- 3oConcentration: Mean Offspring/Female: CONC: 100% Intake Survival andeproduction Data 1 Replicat number Day fi 2 3 4 5 6 7 8 9 10 Y oung produced C7) 0 -- '4 I C Adult mortality t4 J L - ._J_ 2] Young produced To c c) Adult mortality il4_ t L U.._ "Z. 3 Young produced ") Adu lt mortalit y L I ' I L 1 U L [j L Y Y oung produced LA 'A 1_ _1 i Adult mortality I.. 5 Young produced __I'A 14..I~j7. 'I7..~[1. Adult mortality ( Iv k L= dea-) .6 Young produced Ip)oetrti Adult mortalitt 7 [nYoung produced z Ozae: Total young produced 39 3-b 5 1 Z '31-3t --3 3% a-i a Note: Adult mortality (L =live, D = dead) Concentration: % Mortality:
- 1Q7, IMean Offspring/Female:
1 3. % Reduction from Control: I - t % 7.1 I Page 51 of 101
U1I 0~r 0 TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11, 2007 Verification of Ceriodaphnia Reproduction Totals Control-i Day Replicate numbrr Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 6 4 6 4 4
- 4.
4 44 5 12 14 13 11 10 12 13 15 11 12 123 L 6 0 0 0 0 0 0 0 0 0 0 0 7 15 14 18 116 13 16 114 15 17 14 1-52 Total 31 32 35 33 27 34 31 34 32 30 319 11.3% Day '" i' Replicate number Total 4 5 6 8 9 10 1 0 0 0 0: 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0
- 3.
0 0 0 0 0 0 0' 0 0 0 0 4 5 4 5 4 4 4 4 4 4 4 42 5 13, 14 12 13 13 10 14
- 12.
12 11 124 6 0 0 0 0 0 0 0 0 0 0 0 7 15 19 16 17 20 1 1 66 17 15 18 169 Total 33 37 33 34 37 30 34 33 31 33 335 22.6% Day Replicate number 1 2 3 4 5 6 7 8 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0
- 0.
0 4 5 4 5 4 5 5 4 4 4 4 44 5 13 12 14 14 13 15 13 11 14 13 132 6 0 0 0 0 0 0 0 0 0 0 T7 16 19 19 16 15 18 18 19 172 Total 35 31 35 37 37 36 32 33 36 36 348 45.2% Replicate nunber Total Day 1 2 - 3 4 5 6 7 8 1 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2
- 0.
0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 6 4 5 5 4 4 5 5 46 5 13" 15 12 15 13 12 15 16 13 12 136 6 0 0 0 0 0 0 0 0 0 0 0 7 1720 17 18 17 20 18 16 179 Total 34 38 35 39 35 35 36 40 36 33 361 72.6% 7D.a% Replicate number Day 1 2 3 4 5 6 7 8 9 9 0 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 .0 0 0 0 0 4 5 5 4 4 5 4 4 6 6.4 47 5 14 14 16 13 13 15 14 13 16 13 141 6 0 0 0 0 0 00 0 0 0 0 7 1 20 18 18 20 17 19 16 19 183 Total 38 36 40 35 36 39 35 38 38 36 371 1000/0 D Replicate 1lMnber Total 1 2 3 4 5 6 7 8 19 1 T0 1 0 0
- 0.
0 0 0 0 0 0 0 0 2 0 0 0 0 0
- 0.
0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 4 4 5 5 5 4 4 4 6 47 5 15 14 16 14 14 16 15 13 15 15 147 6 0 0 0 0 0 0 0 0 0 0 0 7 17 18 21 19 19 20 17 21 18 20 190 Total 38 36 41 38 38 41 36 38 37 41 384 Control-2 Day. Replicate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 3 4 4 4 .4 5 4 '4 4 40 5 12 12 11 10 10 12 13 10 12 11 113 6 0
- 0.
0 0 0 0 0 0 0 0 0 7 14 14 15 17 15 17 18 17 15 16 158 Total 30 29 30 31 29 33 36 31 31 31 311 1000/% Intake Replicate number Dy 1 2 13.14 5 6 1 7 9 10 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 4 4 3 5 5 3 41 5 14 11 12 14 12 11 12 14 12 124 6 0 0 0 0 0 0 0 0 0 0 0 7 21.18 20 19 17 21 18 19 203 Total 39 33 37 37 33 36 33 38 37 35 358
(I. 0 -5 TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11,.2007 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 3551 Reveiwed by: 4 A Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction froin M 1 2 3 4 5 6 7 8 9 10 (O) (offspring/female) variation pooled controls Control - 1 31 32 35 33 27 34 31 34 32 30 100 31.9 .7.3 Not applicable 11.3% 33 37 33 34 37 30 34 33 31 33 100 33.5 6.6 -5.0 22.6% 35 31 35 37 37 36 32 33 36 36 100 34.8 6.0 -9.1 45.2% 34 38 35 39 35 35 36 40 36 33 100 36.1 6.2 -13.2 72.6% 38 36 40 35 36 39 35 38 38 36 100 37.1 4.7 -16.3 100% 38 36 .41 38 38 41 36 38 37 41 100 38.4 5.1 -20.4 Control - 2 30 29 30 31 29 33 36 31 31 31 100 31.1 6.7 Not applicable 100% Intake 39 33 37 37 33 36 33 38 37 35 100 35.8 6.1 -15.1 Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnett's MSD value: PMSD: MSD = PMSD = 2.152 6.7 1.660 5.3 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10 h percentile) =. 13%. Upper PMSD bound determined by USEPA (9 0th percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). U SEPA. 2001a, 200tb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2-Appendix. EPA-821--B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
CJD Cm TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11, 2007 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 12/4/2007 Test ID: CdFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 31.000 32.000 35.000 33.000 27.000 34.000 31.000 34.000 32.000 30.000 11.3 33.000 37.000 33.000 34.000 37.000 30.000 34.000 33.000 .31.000 33.000 .22.6 35.000 31.000 35.000 37.000 37.000 36.000 32.000 33.000 36.000 36.000 45.2 34.000 38.000 35.000. 39.000 35.000 35.000 36.000 40.000 36,000 33.000 72.6 38.000 36.000 40.000 35.000 36.000 39.000 35.000 38.000 38.000 36.000 100 38.000 36.000 41.000 38.000 38.000 41.000 36.000 38.000 37.000 . 41.000 Transform: Untransformed 1-Tailed I Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 31.900 1.0000 31.900 27.000 35.000 7.307 10 35.300 1.0000 11.3 33.500 1.0.502 33.500 30.000 37:000 6.638 10 -1.700 2.287 - 2.152 35.300 1.0000 22.6 34.800 1.0909 34.800 31.000 37.000 6.028. 10 -3.081 2.287 2.152 35.300 1.0000 45.2 36.100 1.1317 36.100 33.000 40.000 6.187 10 -4.462 2.287 2.152 35.300 1.0000 72.6 37.100 1.1630 37.100 35.000 40.000 4.660 10 -5.525 2.287 2.152 35.300 1.0000 100 38.400 1.2038 38.400 36.000 41.000 5.091 10 -6.906 2.287 2.152 35.300 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.61772913 1.035 -0.0536108 -0.5166972 Bartlett's Test indicates equal variances (p 0.96) 0.98804921 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 2.15229383.0.06747003 57.08 - 4.42962963 2.9E-08 5,54 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL Skew IC05 >100 IC10 IC15 IC20 IC25 IC40 IC50 >100 >100 >100 >100 >100 >100 sqn_]2-04-07data
JIQ M TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11, 2007 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 12/4/2007 Test ID: CdFRCR Sample ID: TVA/ Sequoyah Nuclear Plant, Intake End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 30.000 29.000 30.000 31000 29.000 33.000 36.000 31.000. 31.000 31.000 100 39.000 33.000 37.000 37.000 33.000. 36.000 33.000 38.000 37.000 35.000 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 31.100 1.0000 31.100 29.000 36.000 6.685 10 33.450 1.0000 100 35.800 1.1511 35.800 33.000 39.000 6.148 10 -4.909 1.734 1.660 33.450 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95055902 0.868 0.55299874 0.10323569 F-Test indicates equal variances (p = 0.87) 1.12082267 6.54108953 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic I Test indicates no significant differences 1.66023952 0.05338391 110.45 4.58333333 1.1E-04 1, 18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL Skew IC05 IC10 IC15 IC20 1C25 IC40 IC50 >100 >100 >100 >100 >100 >100 >100 sqnl 2-04-07data
0 -i TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated December 04-11, 2007 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Daily Chemical Analyses 1. Project number: 3551 Reviewed by: Concentration Parameter Da 0 Day 1 Da 2 Day 3 Day 4 Da 5 Day 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final pH (SU) 7.83 7.58 7.381 7.66 7.83 8.00 8.06 8.02 8.10 8.07 8.15 7.99 8.02 8.06 DO (mg/L) 7.7 7.7 7.71 7.91 7.6 7.5 7.71 8.0 7.61 8.01 7.6. 8.1 7.4 7.6 Conductivity (1smhos/cmj 296 31 310 37304 30631 Control Alkalinity (mg/L CaCO3) 57 57 57 Hardness (mg /L CaCO 3) 92 94_92 Temperature (OC) 24.6 24.7 24.7' 24.9 24.7 24.5 24.7 24.6 24.6 24.8 24.8 24.6 24.6 24.9 pH (SU) 7.58 7.57 7.57 7.65 7.67 8.03 8.00 8.02 8.04 8.08 8.09 8.01 8.02 8.06 DO (mg/L) 8.0 7.7 7.6 7.9 7.9 7.5 7.4 7.8 8.0 8.0 7.8 8.1 8.2 7.6 Conductivity (pmhos/cm) 287 315 312
- 3.
293 296 302 Temperature (C) 24.7 24.9 24.7 24.6 24.7 24.5 24.7 24.8 24.7 24.8 24.7 24.9 24.8 24.9 pH (SU) 7.58 7.57 7.56 7.66 7.69 8.03 8.04 8.02 8.05 8.08 8.09 8.03 8.05 8.07 DO (mg/L) 8.0 7.8 7.6 7.9 7.9 7.5 6 7.8 7.9 7.9 7.8 8.2 7.9 7,7 22.6% Conductivity (jsmhos/cm) 280 305 304 320 .285 288 3003 Temperature ('C) 24.7 24.9 24.7 24.8 24.8 24.8 24.7 24.57 24.7 24.84 pH (SU) 7.58 7.56 7.56 7.67 7.70 8.03 8.07 8.06 8.05 &8.09 809 8.05 8.07 8.11 DO (mg/L) 8.1 7.8 7.6 8.0 8.0 7.6 7.6 9 7.9 8.0 8.2 8.0 7.6 45.20 Conductivity (fimhos/cm) 263 287 281 30 267 255 280 Temperature (0C) 24.7 25.0 24.7 24.8 24.8 24.7 24.8 24.5 24.7 24.6 24.7 25.0 24.98 246 pH (SU) 7.54 7.57 7.57 7.69 7.71 8.07 8.08 8.07 8.04 8.16 8.13 8.10 8.09 8.12 DO (mg/L) 8.1 7.8 7.7 8.0 8.2 7.7 7.6 7.9 8.0 8.0 8.0 8.2 8.2 7.6 72.6% Conductivity (gmhos/cm) 241 264 260 268 246 249 248 Temperature (C) 24.8 24.8 24.8 24.9 24.8 24.5 24.8 24.6 24.7 24.9 24.7 25.0 25.1 24.8 pH (SU) 7.511 7.57 7.57 7.75 7.741 8.00 8.11 8.12 8.03 8.16 8.14 8.141 8.14 8.19 DO (mg/L) .8.21 7.8, 7.7 8.0 8.21 7.8 7.71 8.0 8.11 8.11 8.1, 8.01 8.1 7.7 Conductivity (gmhos/cm) .22011112411111111111111 237 250 222923 100% Alkalinity (mglL CaCO 3) 68 72 76 Hardness (mg/L CaCOj) 86 7898 Total Residual Chlorine (mg/L) <0.10 <0.10 ý <0.10 Temperature (C) 25.11 25.0 24.81 24.9 24.9 24.81 24.81 24.6 24.91 24.6 24.7 24.81 25.1 25.01 pH (SU) 7.481 . 7.621 7.611 7.74 7.75 8.061 8.091 8.12 7.951 8.181 8.09 8.121 8.13 8.211 DO (mg/L) 8.4 7.6 7.7 80 8.1 7.6 7.7 .7.8 8.1 81 8.21 8.1, 8.1 . 7.8' Conductivity (tiinhos/cnm) 220 251 .237 249 222 230 223 1000/,, Intake Alkalinity (mg/L CaCO 3) 70 74 74 Hardness (mg/L CaCO3) 84 88 94 Total Residual Chlorine (i <0.10 <0 _Temperature(OC) 25.0 24.71 24.8 j 25.0 24.9 24.7 24.8 .24.7 25.11 224. 24.7 24.9 24.8
V r Environmental Testing Solutions, Inc. Paae 6of 7 Species: CeriodaphlniaI dubia Client: Seauovah Nuclear Plant - Non-treated Daily Chemistrv: Date: vZ-oA-4JI Day 0 I 2 Concentration Parameter 'U.4 ,M CONTROL pH (S.U.) DO (rag/L)
- ")'*;-U".
(jmhos/cm) 4 A.lkalinity N (rgCaCOd)
- q."
Hardness '*AZo0 (mg CaCO3/L) i Temperature (°C) "1A.1 "A.- L -- 1 I__-__ PH (s.u.) Su* ,.c 11.3% DO (mg/L) YJ Conductivity (ýtmhos/cm). Temperature (°C) "L." 'I q,- "AAt W -zA-5 pH (S.U.) c_ ,.L(o 0 ,.c3, 22.6% DO (mg/L) ".) Conductivity (pimhos/cm) Temperature (C) 1--0 2A-41 'L4 - A-zq-k pH (s.u.) q.;* 45.2% DO,(mg/L) 0-.0 -4:X. 5 Conductivity F3 Temperature ('C) 1.". "1. 't. -'-1 0 kA.' pH (S.U.) 72.% DO (mg/L) 1 -0 Conductivity (ýimhos/cm) _Temperature ('C) "-.o R L4. k -Lq. pH (S.U.)Page.57:of 101 Ini F [ InitialF.nal 100% Itk DO (rag/L) 10,2- .3 )- Conductivity (ýimhos/cm)
- {
0- -] Alkalinity (mg CaCO3/L) -10"q Hardness
- 96.
TR chlorine <0.0 Ol (m__[*) P Page 57 of 101 Initial Final I Init I Final II I II I
EaviroDmental Testing Solutions, Inc. Pape 7 of 7 EnirnenalTstn Souios .n.Pg f Species: Ceriodaphnia dubia Client: Seouovah Nuclear Plant - Non-treated Date: \\7-0-- I 7-K Day 4 r Concen-. Parameter-CONTROL 9.0U) ___I1-13% DO (mg/L) Q.Oý: -C (4 -1 1A -Conductivity ~~y ~' 22.6% DCaO3m/L) ,~ f Temperature ('C) I<__ 1 ) _L1_ 241ý 14I 2A ~ 3 j. 11.3% DO (mg/L) b, 0 Conductivity 01 ",4 (pl-nhos/cm)Il -Temperature (T) ZA-" _3Ak 2LA 2A-ZSO _PH(S.U.) 6.02 PCC'j 80- ___3__ e_,0 22.6% DO (mg/L) Conductivity Z( (prnhos/cm)________ Tempeerature ('C) 2MI -LA~ l-. 1Ak.' 14.1 TA__k_4__ Pi R (S U. 61 5 .0 b.0 45.0% DO (mg/L) _____Temnperature (T) 2LI.( 1A ' 24.9 -01.0O 'ti-t'-.(R Is PH "4( U.)i e-oCl ID, .13-12) -7 -UT ,2 1006% DO (mg/L) 079
- 9.
.0 8 - Conductivity A Ternperature ('C) 7(4. 4.1 2T.I ?NI.C 2L..1 L'D.
- 24.
4 I Initial I Final I Initial Final I Initial aInitial a Final I Page 58 of 101
Environmental Testing Solutions, Inc. Page. // Page / of Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0. 10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst I T o6 I Date analyzed /-Z. - Cý/-0 ý lodidereagent: JAk/,' '-o Acid reagent: _p
- o Calibration:
mg[ 1.00mg/L Referencesta number sl / Z ) CS Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) "v B lank (should be <0.10mg/L) cTL~.. 10 tilI7(d= WT'T'" ctQu~dlu'*(p1.,4 btc~n o.oootto% Cn I-ts 0.OS S-I a A/ I0 LI 31F264 -LO.OOOM 2 - OF-01 ItCY 0 "/V Z o a{ Ul 1 10A.O- 0
- 71N.ID~¢ 1
,71,115 Ust~o-V t~totd [cl t4.iýtkba L-0.000002,J M Lb-U 2A kP o Low L-0l "o4, 011 IO )AZ0.03-11 CoI %O, 23. 45&v T',47P 0._*n-Q.,
- .o.*o 3s rcn I-o'b.o L~C 0
V k VcY1 I~ Cn I "k-0).(007O Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reviewed by I 8 I Date reviewed I "IZ - 0 " Page 59 of 101
Environmental Testing Solutions, Inc. Page I Page 2 of Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Date analyzed -0 Calibration: 0.10mg/L I 1.00mg/L Iodide reagent: Acid reagent: Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the. chlorine levels of the samples. Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) Note: sap Blank (should be <0.10 mg/L)s s Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Reviewed by I A Date reviewed 1 17 O I Page 60 of 101
Environmental Testing Solutions, Inc. Page Page ____ of Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0. 10 mg/L Meter: Accumet Model AR25 pH/lon Meter Analyst "P'D!) Date analyzed I2 6-C' Iodide reagent: i:v* Z.C Acid reagent: /,/Vf: 7 Calibration: I <-K~~<<F,*0.10 mg/L i .00 mg/L Reference standard number ,5 1 ///5500 Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV /TV x-100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) /. 1*,/z 0.50 s,. Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD {(S - D) /[(S+D)/2]) x 100 number. (-g/L) (acceptable range +/- 10%) Duplicate D D Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) i ,Blank (should be< <0.10 m ) 1, 7' La b --LOrt coto stanxdard: 4. I6 <Z 0 "T_ / j Reference standard Trevle(TV) Measured value (MV) %/ RS MNV / TV x 100 number og/b) (mg/b) (acceptable range 90 to 110%)11 C ~ '- Z'32~ 0.50 S - S'4.Z /<9 *'2, I I I I I Reviewed by Z1 I-0 Date reviewed I Page 61 of 101
Environmental Testing Solutions, Inc. 0L C/ Page I___ Page I of 3 Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Date analyzed LiimOzpa Iodide reagent: TNrql.0Alo Acid reagent: j-zrtzX0 Calibration: I:**.,:**!.
- .+
- ,* :.>:¢ 0.10 mg/L 1.00 mg/L Reference standard number
.Z NMs,%. Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) 0.50 O.bOq ILI Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number _(mg/L) Blank (should be= <010 rmg/L) Z- 0* 0.00 -10"1 0-7 V-Lng 03 3y~____________~cOS o-1 -z 6,-o a 0&d % e SS
- _kFclJVuWA&.N O.oo, 0
I 1 O1~ZO~3/4 S4-rb~i~~ ".Ol0010 a-1 z.oo0.o8 0 uA SS kc-,No Colovo.oOS o-"1120.o U C-oXt L110N Pl,cU .-t_ ,NO c6tV 0.00S3 7 a-1 iLpi,\\ I C ro- 0 VMV'0P ct4 lo
- {1+/d<,
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- 0. oa a a l 'z o &. o \\
1 I Ul t, po-i c s, Lo. o I N I o I"t.OR.G E o n-CA CAC. J to aLj'Y ,o.*O1 0 -001 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted, Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) Reviewed by zI SIIZ---- Date reviewed 12 - 0 Page 62 of 101
Environmental Testing Solutions, Inc. Page I Page of 3 Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0.10 mg!L Meter: Accumet Model AR25 pHiIon Meter Analyst Date analyzed 1 3.-Oý - C)-1 Iodide reagent: Acid reagent: Calibration: 0.10 mg/L 1.00 mg/L Reference standard number Note. For samples with a residual chlorine of >.1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV!/TV x 100 number (rg/L) (mg/L) (acceptable range =90 to 110%) -rsS 0.50 o.MSTJ 9: 1 C.I-Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/_) -_______ Blank (should be=< 0.10 mg/L) `., 0o7 12xl -I 4,
- ,rav W v,4 rTP crtap. poa-. *
-o.ooi:3 oC1 zoR, VAc. V dCR (A/*d-.o d ,O31 t t U0" NO C-~O0 000b9 az cri~~~~~to Io-c .aloosd* &Coo al %z* ZrJ- .CA Zy*-&q;0Ya i N,'W'V'P C~ttax, ne~tt k-0-N Crlvy-Aik ipt~ tr~cý rAOUA-WW(Vo)Uxide-s. -"ý4#rA ~ witc~ ,-1Th(1.o 5N A CAt, uoý.L,
- ( l.oalc-tes
.'1, 0 Note: All samples were analyzed in excess of EPA recommended holding time (5 mutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS =MV / TV x 100 number (mgfL) (ag/L) (acceptable range = 90 to 110%) Reviewed by Date reviewed Page 63 of 101
En~vironmental Testing Solutions, Inc. Page _ Page of 3 Total Residual Chlorine (Orion ElectrodeMethod, Orion 97-70) Matrix: Water, MDL = 0. 10 mg/L Meter: Accurnet Model AR25 pH/lon Meter Analyst Date analyzed I a i Iodide reagent: Acid reagent: Calibration: 0.10 mg/L .1.00 L*g. Reference standard number I8 d Note. For samples with a residual chlorine of > 1.0 rag/L, the calibration range must be adjusted to bracket the chlorine !evels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (rag/L) (mg/L) (acceptable range = 90 to 110%) [,t$c~ Bt 0- .50 O.*t(pq 92 5. 1 Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L2 B lank (shouldbe(<.10mg/L) (mg ji (ae t
- al 120,1.-
o.5o o S-:N3 t* CA* o~O4
- . * ¢o. M Th:~::i~
5CN-4o~L CrfYj-ý Cýo5 uA 0" t -OJ 0cU- ?Ie-ltý a1O2NJ.. lot tkt n -k -i Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: [ Reference standard True value (TV) Measured value (MV) %/ RS MV/TV x 100 [number I(mg/L) (mg[L) (acceptable range 90 to 110%) S0.50 0.$I~ Cam01 Reviewed by Date reviewed 17-0 0 Page 64 of 101
PaPe ge f Page -- L.-of Environmental Testing Solutions, Inc. Alkalinity (SM 2320 B) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Time initiated [ Date analyzed Titrate samples to Time completed / -b 3 pH = 4.50 S.U. Titrant normality andm ultiplier determination:- pH of Normality Normality (N) of H2SO pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml NalC0 3 x 0.05)/E = (N x 50000)!100 ml sample water reference standard nil ml ml = 0.25/E N x 500 4.5 S.U. number number (E) (acceptable range 0.018,- 0.022) '.c* 1*0-0 C ,,3,**C . i0. Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO3/L) (ml) nil nl ml = 90 to 110%) 'A 100 100 M.2, t
- 9.
/t).*) o0 7.,O/. Duplicate sample precision: Sample Alkalinity %RPD Sample. Sample ID volume Begin End Total Multiplier (rag CaCOi/L) f(S - D) /{(S+D)/2]} x 100 number . (nil) al ml ml ,(acceptable range =10%) I'-,up, :c '9 )l$. icate (B) DJ. )').5 "'- { ,..K) Duplicate(B) /
- "T.}-
5?.2 D -.- 4_--- .*17 Samn1~ mpnejrpmpnic Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (rng CaCO31L) l,.t46.*i4 '-iO q*: Ii').C [O0 .&,) ~.,7 "52-I_____. r55j7 /b/. &,,7 1; id~t '7o/. I/_________,_-___ . 3
- 7.,.
a*e*<.* ,4;ý 1o
- 85. 6 9
E.) i;d*-':- ..,."-Ldt /u. 37,11 1'ý.q I"i Reviewed by: Date reviewed: [ Page 65 of 101
Page TI Environmental Testing Solutions, Inc. Page a - of Analyst L Date analyzed Alkalinity (SM 2320 B) Matrix: Water, MvDL = 1.0 mg CaCO 3/L Titrate samples to pH = 4.50 S.U. Time initiated Time completed T:~...,..f,,,-,.-na li/v and mnltinlipr dptprmination: ~ pHrf---- -Normality Normality (N) of H2SOj pH Factor or Multiplier Deionized Titrant check -tal'- i( -- End----.al (5 ml NaICO 3 x 0.05)fE = (N x 50000)/ 100 ml sample water reference standard ml ml ml " = N x 500 4.5 S.U. number number (E) (acceptable range = 0.0i8 7G022r--- Laboratofy control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV /TV x 100 number (TV) volume Begin End Total Multiplier (rmg CaCOjtL) (acceptable range (mg CaCO3L) (ml) ml ml ml= 90 to 110%70) 1PCS~J100 100 3e p u Duplicate sam le precision: Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCOJL) {(S -D) /[(S+D)/2]) x 100 number (ml) ml ml ml (acceptable range +/-10%) Duplicate (B) j D Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOJL) (mg CaCO3/L) (ril) ml ml ml i3, Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCOJL) MV = A - B (acceptable range (mg CaCO3/L)' 75 to 125%) u s--. Sample measurements: Sample volume Begin End Total Alkalinity. Sample number Sample ID (ml) ml ml mnl Multiplier (mg CaCOJL) Ljqq'U) 5i.S. g /Ot,>~,Z ioo .,n 8.5 .3.5 ,5.5-Is:j/.f)
- )
._S / ,D,'3.o 6.o .e L-1!I Q 3t. 3- -0
- 315, I 3..(',L.,.,9-
- x_
I/a.o-MAY., /Io <1L 7tz O 1.o (7 L,O ', L 5, / d -OQ iI,O / '.-) *. h:*) I Lflt2o's. '-1 7 /'.5 +. I ~ 'ZC1B j~ Reviewed by: Date reviewed: [ -21.C1 I Page 66 of 101
Page Page ffIof1 f Environmental Testing Solutions, Inc. Alkalinity (SM 2320 B) Matrix: Water, MDL = 1.0 mng CaCO3/L Analyst Time initiated Date analyzed iz 2 ,G! *' Titrate samples to Time completed pH = 4.50 S.U. Titrant normality and multiplier determination:. pH of Normality Normality (N) of HSO4 pH Factor or Multiplier Deionized Titr i-----elck-Begin End Total = (5 ml Na2 CO 3 x 0.05)/E (N x 50000)/ 100 ml sample water reference standard ml = 0.25/E = N x 500 = 4.5 S.U. number number (E) (acc bte-rpge-=-L0-0 -80.022) Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L), (acceptable i-ange (nbg CaCO/L) (ml) ml ml ml = 90 to 110%) Duplicate sam le precision:_ Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO3!L) {(S -D) /[(S+D)/2]) x 100 number (ml) ml ml ml (acceptable range t 10%) 1 DZOub.O c ! (B ) )
- .)
") , I C Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOJL) o L Os -.LS 2-3.Z 3'4. U- ~.3 i Y l)- j9..
- 10. e 2n 1-(9, Ll
(!SL cf1c oC1 O~o t Reviewed by: w Date reviewed: L i e" Page 67 of 101
Pace "'/; Page -i of Environmental Testing Solutions, Inc. Alkalinity (SM 2320 B) Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst }J,- Time initiated Date analyzed Titrate samples to Time completed pH = 4.50 S.U. Titrant normality and mutihplier determination: pH o Normality Normality (N) of HSO4 pH Factor or Multiplier water reference standard ml ml ml =0:25/I._ N x 500 4.5 S.U. number number (E) (acceptable range = 0.018- 0.02.5-........J Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % ES = MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJL) (acceptable range (rag CaCO3/L) (ril) ml ml 90 to 110%) DuLlicate sam ple precision:. Sample Alkaliriity %RPD .Sample. Sample ED volume Begin End Total Multiplier (mg CaCOJL) ((S -D) /[(S+D)/2]} x 100 number (ml) ml ml ml /'3, S (acceptable range 10%) Duplicate(B) V. ./(,1 D Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L) (mg CaCO3/L) (ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) '% R = MV / SV x 100 (mg CaCO3/L) MV = A-B (acceptable range (nmg CaCO3./L) =75 to 125%) 340 9O9.7 Sample measurements: S Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3IL) on i-Zon. I I P( -.t*. /- &S q4_4 3.-*..0 ) /O b-0 q *0,o'So i rcv.h oo(z !oe
- 0. -u.q
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- i..
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- )
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P-age 77 Page ___j Environmental Testing Solutions, Inc. Alkalinity (SM 2320 B)
- Matrix: Water, ivfDL = 1.0 mg CaCO31L Analyst "
LV& Time initiated Date analyzed t Titrate samples to Time completed pH = 4.50 S.U, Titrant normality and multiplier determination: -pH-- Normality Normality (N) of H2SO pH Factor or Multiplier Deionized Titrant egin--.End. Total = (5 nil Na2CO 3 x 0.05)IE (N x 50000)! 100 nil sample water reference standard ml ml .l. .... =0.25/E.. =Nx 500 = 4.5 S.U. number number - _(E) (acceptable range 0.018 - 0.022) Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (rmg CaCO3/L) (acceptable range (rmg CaCO3/L) () l ml ml= 90 to 110%) ..:S,.-, t-100 U0 '94 (,L J(. I L j i. Duplicate sam le precision: Sample Alkalinity %RPD = Sample Sample ID volume Begin End. Total Multiplier (rag CaCO3fL) {(S -D) /[(S+D)/2]} x 100 number ni(ml) ml "ml ml (acceptable range t-10%) D~*._ Duplicate (B) Ds~ / _____9 Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) nl ml ml Multiplier (mg CaCO3/L) (31 i-Zc)'.* 7/-'sQz,*[3/4) / .5j3 43.2 3,, z-* /4.12) -t -zoS.t1-
- o.
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- zo.,
i.S .3.cO S. 0112 _C1 .1Z)_ .'3 ,2-CA-oC- .2__ ~.z. [*o-q ,5.1 Reviewed by: m Date reviewed: I t-21-0-1 Page 69 of 101
Page Page f of Environmental Testing Solutions, Inc. Alkalinity (SM 2320 B) Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst Time initiated Date analyzed. Titrate samples to Time completed pH = 4.50 S.U. Titrant normality and multiplier determination: pH of Normality Normality (N) of H2SO pH Factor or Multiplier Deionized Titrant check Begin End Total (5 mil Na2CO 3 x 0.05)/E = (N x 50000)!100 ml sample Ta-t-r - reference sa-n-f arr -ff--M---ml------- 0.25*E =N x 500 = 4.5 S.U. number number (E) (acceptable range = 0.0T8---02-2)-- Laboratory control standard: Reference standard True value Sample Alkalinity (MV) % RS MV TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOIL) (acceptable range (mg CaCOjIL) (ml) ml ml rl = 90 to 110%) 00__ 100 6! 3e,1/2s¢* 4C) Vo 2c: 1?z 3 ID!* K) C3o/." Duplicate sam ple precision: Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mgCaCO3/L) (iS - D) /[(S+D)/2l} x 100 number (ml) ml mln ml (acceptable range t 10%) Cri ~ ~ ~ I -0 LA 0I U 0 it0 2 Duplicate (B) "G
- O D
,,40,. Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOa/L) (mg CaCO3IL) (ml) ml ml ml 07,-1
- 0.
lO!3 M-_3 io -co 0 Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (rg CaCOVIL) MV = A - B (acceptable range (mng CaCO3jL) 75 to 125%) Samle measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mng CaCO3 L) C zi ta-a4, C 2 /00 10.3 (_3
- .;.o 5 -16 071 20-.0 3
153I Ao1.0__ n 12'6 00.3q.o ~ 1 2 LEI~z8 12 05 -_ 1_ (0 _7_ Reviewed by: Date reviewed: 1l-Z. 1 Page 70 of 101
'nvironmental Testing Solutions. Inc. Pie of Total Hardness (SM 2340 C) Matrix: Water, MDL = 1.0mg CaCO 3/L Analyst Time initiated Date analyzed t i 7:O -:,) Time completed Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard mn] ml ml = 0.2fE = (N x 50000)/ 50 nml sample number number (E) (acceptable range- 0.018 -0.022) N x 1000 Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS = MV! TV x 100 number (TV) volume Begin End Total Multiplier (rug CaCO1JL) (acceptable range (mg CaCOi/L) (ml) ml ml ml 90 to 110%) 40 50 -;j,
- . j
'// Duplicate sam ple precision: Sample Hardness %RPD = Sample Sample ID' volume Begin End Total Multiplier (rug CaCOJL) {(S -D) /[(S+D)/2]} x 100 number (ml) ml m ml nd (acceptable range t 10%) Duplicate (B) D Matrix spike recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (rug CaCOy'L) (rug CaCOi/L) (ml) ml ml ml Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (rmg CaCO3/L) MV = A -B (acceptable range (rug CaCO3IL) = 75 to 1250o) qq qo, oy/ Sample measurements: Sample volume
- Begin, End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOg'L)
Blank d ) g,. (should be = 0 mg CaCO3/L) N.0 I
- - /
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Page of P a ge o f Environmental Testing Solutions, Inc. Total Hardness (SM 2340 C) Matrix: Water, MDL-1.0 mg CaCO3/L Analyst Time initiated Date analyzed /..-,-.- Time completed Titrant normality and multiplier determination: - Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml nd ml = 0.2EA (.V, 50000) 50 n-d sample n n-be ( ceprabe-rn ,.022) "N x 1000 Matrix spike recovery:, Reference standard Spike value Sample Spike hardness'(A) number (SV) volume Begin End Total Multiplier (nmg CaCO 3/L) (mig CaCO3yt) (1-11) nil nil mlI W
- 0.,q S3 c,.3
'30 Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCOy'L) MV = A - B (acceptable range S(mg CaCOIL) 75 to 125%) 8 '420 Sample measurements.- Sample volume Begin End Total Hardness Sample number Sample ID (nl) ml ml ml Multiplier (Og CaCOJL) (should be = 0 mg CaCO3/L) al 1 0:5 '15 oq a o 3/4'~~'zq 3*
- _.3;2 Jo
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- 01. lu,* i) iv'Y/61; -#
rd ) 1 5:1 o I A an_______ Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: Page 72 of 101 Date reviewed [ 2-..(1
Pae
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Environmentai Testing Solutions. Inc.
- Total Hardness (SM 2340 C)
Matrix: Water, MDL = 1.0 mg CaCO3/l Analyst Date analyzed Time initiated Time completed Normality (N) of EDTA = 0.2/E (acceptable range = 0.018 -0.022) pH Factor or Multiplier - (N x 50000)/ 50 ml sample = N x 1000 Matrix spike recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (rag CaCOIL) (mng CaCOj/L) (ml) ml ml Sample hardness (B) Measured spike value (MV) % R = MV /SV x 100 (ig CaCOpe L) MV = A-B (acceptable range (rag CaCOy'L) = 75 to 125%) Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (rng CaCO3/L) Blank ,t.. Td-* O (. r ,--1,--o ao..Z*.~. ,[. 7/ 4,, l t9I*.t j}* t, m U" Irzoi. 0 3m 4),, Cn 1Al~ 123 0 le.i 0Al T ~ I~ Page 73 Note: If >15mI of titrant is used, sample must be diluted. of 101. Reviewed by: E Date reviewed 1 %'2-'I26 I
IEnviron3mental Testing Solutions, Analyst I ~Date analyzed Titrant normality and m ult lier determinat I Titrant Normality check Begin E referec.e _ standard m r number nUM-b-r Laboratory control standard. Reference standard True value Sample number (TV) volume (nmg CaCO3/L) (ml) I 40 50 Duelicate sam le precision. I Sample Sample Sample ID volume number (ml). Duplicate. (B) I IMatrix spike recovery: Reference standard Spike value Sample number (SV) volume (mg CaCOL) (mnl) Sample hardness C (rmg CaCO3JL) Sample measurements: ISample number Sample iD (should be = 0 mg CaCO3/L) I on l1 o'j. o3 \\, 5,J 0"1 Q 015- 0. -1"2"...2 17Z" OS..- crn* c Y 0 S. T'L-3
- ,J I
(TI I -Z 0*1 Notej If >lSml of titran is used, sample must be diluted. Page 74 of 101 Pac Page _______'+ of [nc. Total Hardness (SM 2340 C) Matrix: Water, MDL = 1.0 mg CaCO 3/L Time iniiated Time completed t;on: nd Total Normality (N) of EDTA pH Factor or Multiplier nI ml = 0.2/E = (IV. 50000)/ 50 nil sample ().. .(acceptable range = 0.018 - 0.022) = N x 1000 Hardness (MV) % RS = MV / TV x 100 Begin End Total Multiplier (mig CaCOJL) (acceptable range ml ml ml 90 to 110%) Hardness %RPD Begin End Total Multiplier (rag CaCO31L) {(S - D) /[(S+D)/21) x 100 ml ml ml (acceptable range +/- 10%) _s
- ..,.e,*o*.
4t., 4" D,; -. 11 ý4 el n. Sample volume Begin End Total HIardness (ml) ml ml ml Multiplier (mg CaCOJL) 50 4, LtII iL q*.tf ?.. , _1 /_ ~ l 2.1 qi-j.j_ Reviewed by: Dat Date reviewed I k-L- -ZO.-
Pa e of Environmental Testing Solutions, Inc. Total Hardness (SM 2340 C) Matrix: Water, MDL = 1.0 mg CaCO3 /L Analyst1 K', Date analyzed K)-,. Time initiated Time completed ant normality and multiplier determination: Normality (N) of EDTA p11 Factor or Multiplier (N x 50000)/! 0 ml sample ceptable range =.01-is--)X2"-'- - = Nx 1000O Laboratory control standard.' I Reference standard True value number (TV) (nmg CaCOAL) Sample volume. Begin (Mr) ml , -40 50 Sample measurements.- Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml nil, ml _Multiplier (Tn CaCO3/L) (should be 0 mg CaCO3/L) - 6 CnJ i V 4 f Ai,
- -tL 8
6"1*,-2. C"J a j,.- SPage 75 o t~Oj:If >15ml of titrant is used, sample must be diluted. Reviewed by: Date reviewed,
Sequoyah Nuclear Plant Biomonitoring December 4-1 1, 2007 Appendix D Reference Toxicant Test and Control Chart Page 76 of 101
tnvironmentai i estlng LOiulons, Inc. Pimephales promelas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water Organism Source: Aquatox, Inc. I.2Z 1.0 F-I I I I I C nI [oI I I S t USEPA Control Limits (+/-.2 Standard Deviations) 0.8 F-0.6 F-0.4 1.2 tt) 1.0 0.8 t I I I I I I I I I I I I I th -th USEPA Warning and Control Limits (75 and 90 Percentile CVs) 0.6 0.4 1.2 1.0 0.8 0.6 0.4 j I I I I I I I I I I I I Laboratory Warning and Control Limits (10th and 25th Percentile CVs) I I [I I I I I I I I" I I I I I I Test date 7-day I C25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC25) Warning Limits (mean IC 25 +/- S A.10 or SA75) Control Limits (mean IC25 +/- S 25, SA-90, or 2 Standard Deviations) Page 77 of 101
a; --4 0o Environmental Testing Solutions, Inc. Pimephales promelas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water Organism Source: Aquatox, Inc. State and USEPA Laboratory Laboratory S Control Limits SA.10 Wanting Limits Sý25 Control Limits CT-2S CT+2S CT - So CT + Skto CT - S,,~ CT + S,,, Test number Test date 7-day ICs CT (g/L KCI) (g/LfKCI) USEI'A SA.1s Waning Limits CT - S,,, CT + S,,, USEPA SA9o Control Limits CT - S, 9o CT + Sýgo 2 3 4 5 6. 7 8 9 10 11 12 13 14 15 16 17 18 19 20 02-20-07 0.59 03-06-07 0.71 04-03-07 0.53 04-03-07 0.79 05-08-07 0.64 05-30-07 0.62 06-05-07 0,77 07-10-07 0.73 07-17-07 0.80 07-31-07 0.68 08-07-07 0.67 08-14-07 0.64 09-11-07 0.61 10-02-07 0.67 10-09-07 0.64 10-16-07 0.66 10-30-07 0.60 11-06-07 0.72 11-13-07 0.72 12-04-07 0.52 0.65 0.61 0.66 0.65 0.65 0.67 0.67 0.69 0.69 0.68 0.68 0.68 0.68 0.67 0.67 0.67 0.67 0.67 0.67 0.09 0.48 0.83 0.08 0.57 0.09 0.42 0.80 0.07 0.54. 0.12 0.42 0.90 0.08 0.58 0.10 0.45 0.86 0.08 0.58 0.09 0.46 0.84 0.08 0.57 0.10 0.47 0.86 0.08 0.59 0.09 0.49 0.86 0.08 0.59 0.10 0.49 0.88 0.08 0.60 0.09 0.50 0.87 0.08 0.60 0.09 0.51 0.86 0.08 0.60 0.08 0.51 0.85 0.08 0.60 0.08
- 0.51 0.84 0.08 0.59 0.08 0.52 0.83 0.08 0.59 0.08 0.52 0.83 0.08.
0.59 0.07 0.52 0.82 0:08 0.59 0.07 0.52 0.82 0.08 0.59 0.07 0.52 0.82 0.08 0.59 0.07 0.53 0.82 0.08 0.59 0.08 0.51 0.82 0.08 0.59 0.73 0.68 0.74 0.73 0.73 0.75 0.75 0.77 0.77 0.77 0.76 0.76 0.76 0.75 0.75 0.75 0.75 0.75 0.75 0.14 0.52 0.13 0.48 0.14 0.52 0.14 0.52 0.14 0.51 0.14 0.53 0.14 0.53 0.14 0.54 0.14 0.54 0.14 0.54 0.14 . 0.54 0.14 0.53 0.14 0.53 0.14 0.53 0,14 0.53 0.14 0.53 0.14 0.53 0.14 0.53 0.14 0.53 0.79 0.74 0.80 0.79 0.78 0.81 0.81 0.83 0.83 0.83 0.82 0.82 0.82 0.81 0.81 0.81 0.81 0.82 0.81 0.25 0.23 0,25 0.25 .0.25 0,25 0,26 0,26 0,26 0.26 0.26 0,26 0.26 0,26 0.26 0.25 0.26 0.26 0.25 0.40 0.38 0.41 0.41 0.40 0.41 0.42 0.43 0.43 0.42 0.42 0.42 0.42 0.42 0.42 0.41 0.42 0.42 0.41 0.90 0.29 0.84 0.28 0.91 0.30 0.90 0.29 0,89 0.29 0.92 0.30 0.93 0.30 0.95 .0.31 0.95 0.31 0.94 0.31 0.94 0.31 0.93 0.30 0.93 0.30 0.93 0.30 0.93 0.30 0.92 0.30 0.93 0.30 0.93 0.30 0.92 0.30 0.36 0.34 0.36 0.36 0.36 0.37 0.37 0.38 0.38 0.38 0.37 0.37 0.37 0.37 0.37 0.37 0.37 0.37 0.37 0.95 0.13 0.89 0.15 0.95 0.18 0.95 0.16 0.94 0.14 0.96 0.15 0.98 0.14 1.00 0.14 0.99 0.13 0.99 0.13 0.99 0.12 0.98 0.12 0.98 0.12 0.98 0.11 0.98 0.11 0.97 0.11 0.97 0.11 0.98 0.11 0.97 0.12 Note: 7-d IC5 s 7-day.25% inhibition concentration. An "estimation of the concentration ofpotassiuni chloride that would cause a 25% reduction in Pirnephales growth for Uie test population. CT Central tendency (inean IC2_). S = Standard deviation of the IC2, values. Laboratory Control and Warning Limits Laboratory control atd warning linits were established using the standard deviation of the IC,5 values corresponding to the 10th and 25th percentile CVs. These ranges are morc stringent than die control and wanting limits recommended by USEPA for the test method and endpoint. Suo = Standard deviation corresponding to the 10h' percentile CV, (S,t = 0.12) Sxs ý Standard deviation corresponding to the 25'h percentile CV. (SAt25 = 0.21) USEPA Control atid Warning Linits S175 ý Standard deviation corresponding to tie 7 5th percentile CV. (S7t = 0.38) Sx9o = Standard deviation corresponding to the 90t' percentile CV. (S_9 o = 0.45) CV = Coefficient of variation of tre IC25 values. USEPA. 2000. Understanding astd Accounting for Method Variability in Whole Effluent Toxicity Applications Under the Natioatl Pollutant Discharge Elinhýiation Program. EPA-833-R-00-003. US EnvironnmentalProtection Agency, Ciocinnati, OH. Organi-ms obtained from Aquatox, Inc. atox12-04707
Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Pimeph ales promelas / Potassium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Organism Source: Aquatox, Inc. Test number Test date Control Control Mean CT CV CT MSD PMSD CT Survival Growth for Control Growth for Control N) (mg/larvae) (mg/larvae) N Growth CV (%) for PMSD 1 02-20-07 100 0531 6.3 0.12 22.8
- 2.
03-06-07 100 0.734 0.632 15.6 11.0 0.12 15.8 19.3 3 04-03-07 100 0.724 0.663 14.8 12.2 0.11 15.8 18.1 4 04-03-07 100 0.763 0.688 3.7 10.1 0.12 15.9 17.6 5 05-08-07 100 0.883 0.727 6.1 9.3 0.09 10.5 16.2 6 05-30-07 100 0.790 0.737 12.2 9.8 0.11 13.6 15.7 7 06-05-07 100 0.644 0.724 11.9 10.1 0.11 17.1 15.9 8 07-10-07 100 0.693 0.720 2.3 9.1 0.05 7.5 14.9 9 07-17-07 100 0.685 0.716 8.5 9.0 0.08 12.2 14.6 10 07-31-07 100 0.686 0.713 7.9 8.9 0.08 11.0 14.2 11 08-07-07 100 0.854 0.726 11.5 9.2 0.13 15.7 14.4 12 08-14-07 97.5 0.900 0.740 13.1 9.5 0.13 14.4 14.4 13 09-11-07 100 0.831 0.747 6.3 9.2 0.14 17.4 14.6 14 10-02-07 100 0.918 0.760 4.9 8.9 0.17 18.1 14,8 15 10-09-07 100 0.772 0.760 7.9 8.9 0.08 10.2 14.5 16 10-16-07 100 0.662 0.754 13.6 9.2 0.08 12.8 14.4 17 10-30-07 100 0.771 0.755 12.0 9.3 0.16 20.1 14.8 18 11-06-07 100 0.624 0.748 6.4 9.2 0.09 14.7 14.8 19 11-13-07 100 0.603 0.740 14.2 9.4 0.10 16.8 14.9 20 12-04-07 100 0.756 0.741 7.9 9.4 0.11 14.5 14.8 Note: CV = Coefficient of variation for control growth Lower CV bound determined by USEPA (10'h percentile)= 3.5%. Upper CV bound determined by USEPA (9 0ih percentile) = 20% MSD - Minimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10' percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile) = 30%. CT= Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program, EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 20 01a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtained from Aquatox, Inc. atoxN 2-04-07 Page 79 of 101
tnvironmentai - esnng koiutions, inC. Pimnephalespromelas Control Growth, Coefficient of Variation, and PMSD in Potassium Chloride Chronic Reference Toxicant Tests Organism Source:. Aquatox, Inc. Z 1.25 1.00 0.75 0.50 0.25 I I l [ I USEPA Acceptance Criteria (> 0.25 mg per surviving larvae) I I I I I t I r 0 0 0 0 0 30 20 10 0 I I I7 I I I I I I I I - I I I I I Kentucky Acceptance Limit (< 30.0%) I I I I I I I I I.I.. .....I. .I 30 20 10 A kJ z' Q'I z' Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Page 80 of 11j1ntrol Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)
Environmental Testing Solutions, Inc. Page I of 5 Environmeidal Testin2 Solutions. Inc. Page 1 of5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimeph ales promelas PpKCICR Test Number: Dilution preparation information: Comments: KCI CHM number: C___K______ Stock preparation: 50 g KCI/L: Dissolve 50 g KC( in I-L Deionized water Dilution prep (mg/L) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Test information: Organism age: '2 t - Vbu4S. Atlý Randomizing template: eut~no -se !Ifj Date and times organisms %L - fl thpb 1 Incubator number and were born between: shelf location: Organism source: PITh'b. 6A-TCi Pp %-Z-0o*-. Artemia lot number: .'tuq \\j Transfer bowl information: pH = SU Temperature = 'C Total drying time: "_-_O__%_ "1.' "~Lb _ Date/.Time in: %15-a\\-a Average transfer volume: Date / Time out:.1r.-d1-L t25o Oven temperature: IQE".- Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, MHS Analyst feeding feeding time renewal, or batch used time termination time 0 0-1
4 1 S li:<
-z\\-cx1 A 2 Q2-016-0"1 o~ o N4S v0\\3 x.A-as C1 3 I-l.- "--a-} 02S Ilk_______ ZQ.o1-of o 6 11 6 -6 4 oti-+ 1A -L ILi40 Control information: Acceptance criteria Summary of test endpoints: % Mortality: S7.
- < 20%
7-day LC50 , \\ Average weight per initial larvae: a.-I(, NOEC Average weight per surviving larvae:
- 0. IT S 0.25 mg/larvae LOEC ChV IC25 s zo -q I
Page 81 of 101
Environmental Testing Solutions, Inc. Page 2 of 5 Environmental Testing Solutions. Inc. Page 2 of 5 Species: Pimeph ales prom elas PpKCICR Test Number: - 130 Survival and Growth Data Day Control 450 mg KCI/L 600 mg KCI/L A B! C ID E F G IH I J 0 O O o 0 /1(3! 5 O /b /0 0 /C 10 1 /Q /0 /0 /0 /1 /O /0 C0 2 IQ (0 I 0 /0 l)/(. QO0 /0 t /0 1/ lO 3 J (0 /0 10 10 10 1o
- 0
/1 10 ClO)A. D Ic /) 0 10 10 1t2 1 I0 /0 10 1(3 ' I 6 A t10 /0/ /0 JO C) /0 /0 /0 q 7 7-V t0o 9 /0 t1 10 1U. I * -1 SA_ A Pan weight (mg) Trayecolor code:: p, _\\%__. 14te!I.0 1 -0 IcaAc I q.31 13:11 V4,"1q V"S.S3,5.31 13ý.510
- .1, t4.1 Analyst:
- L
- qI
.53 B Pan + Larvae weight (mg) Larv -L.e I Ze.igh t7m*a 1eiZght (mg)qZý,Z, IS-c 1., 11,oq 1".So Analyst: MA4 C = Larvae weight (mag) =A-B Weight per initial number of larvae (mg) = C/Initial number of larvae ID. 1V1 'p 0" N 0 0* 0* 0" 01 Average Percent weight per reduction initial from control number of (%) larvae (mg) -0., Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w wounded. Calculations and data reviewed: Comments: Page82 of 101
Environmen'tal Testing Solutions, Inc. Page 3 of 5 Species: Pimephales promelas PpKCICR Test Number: 1.A Survival and Growth Data Day 750m KC/L00 KCI/L 1050 mg KCI/L M N 0 P Q R SIT U V X 0 t(3 10 1t5 L /( / 5 /I IQ /0 /0 [ao /0 A A 6q -k IA' 44 J4, SS CA' Ci S I -I (D (D 2 d 1 S A "2 3 eq n -14 to S, 4 8` t'%sa*A
- &, 2.kIa 5
L~ O~ ~s g, 0 A 2.e A = Pan weight (mg) Analyst: A B =Pan + Larvae weight (mg) ~j).l~ ~ g,4 ~ ~ 1~ i7 ~3-341 Larvae weight (mg) A-B*- 4-i11 3.) i.%.o 2.4 "Z.$1 3.10 v.-S a.2-o-t' Weight per initial numberoflra m).* I%\\tt ,0 (* v
- V C/=
PInitial number of larvae weigh Average Percent weight per reduction in itial from control s%"*
- c. *...
7.2.O( .~I.* a O. 0 dl0-.!* number of (%) larvae (ma,) Comment codes: c = clear, d = dead, fg fungus, k = killed, m missing, sk = sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w wounded. Calculations and data reviewed: Comments: Page 83 of 10
4Z M Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephalespromelas Organism Source: Aquatox, Inc. Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: PpKCICR #139 Test dates: December 04-11, 2007 Reveiwed by: Cnnntr-ation (mg(L Rtplite Initiol n.-ber of Final nunber of A - an wenight long) L.oe-weighl (t.&) Woighl I Surivisng Moon.oighl) Coonlinin of varittIon Weight I mber Meanno onivat Mran weightlnibol P-ornt rodutinn ffom KCI) tIr.-e I.r-ae weight (trg) - A - B nomber of 0I.-na (.og) Srviviong nuber of I ( tl,, I or 0 of la-non (rag) (%) nu erof atoa-no cariation (%) control (o) l1t-Cot (nig) 0.... ,Ooolon.) (0/) (tg) A 10 10 14.69 23.01 8.32 0.832 0.832 Control B 10 9 14.09 21.11 7.02 0 0.775 6.2 0702 97.5 0.756 7.9 Not applicable C 10 10 .15.95 23.09 7.14 0.714 0.714 D 10 10 14.31 22.05 7.74 0.774 0.774 E 10 10 13.71 19.89 6.18 0.618 0.618 450F to0 10 13.74 20.06 6.32 0.632 0.687 1(.8 0.632 100.0 o.687 11.8 9.1 G 10 10 13.53 20.54 7.01 0.701 0.701 H 10 t0 15.31 23.26 7.95 0.795 0.795 I 10 10 13.56 18.50 4.94 0.494 0.494 600 10 9 15.92 19.26 3.34 0.371 0.502 20.3 0.334 87.5 0.433 16.3 42.7 K 10 9 14.33 19.04 4.71 0.523 0.471 F L 10 7 13.47 17.80 4.33 0.619 0.433 M 10 7 14.50 18.67 4.17 0.596. 0.417 N 10 6 13.86 17.33 3.47 0.578 0.347 150 0151551832.00500.527 19.6 02060.0 0.31.7 26.3 58.0 0 ( 0 5 15.53 (8.33 2.80 0.560 0.280 P 10 6 12.90 15.14 2.24 0.373 0.224 Q (0 5 .14.43 16.80 2.37 0.474 0.237 R 10 4 14.38 16.08 1.70 0.425 0.462 I 0.170 S 10 3 14.31 15.57 1.26 0.420 0.126 42.5 0.200 31.7 73.6 T 10 5 14.60 17.25 2.65 0.530 1 0.265 1 U (0 1 13.61 13.73 .0.12 0.000 0.012 1058 V 10 0 0,00 0.00 0.00 0.000 0.000 0.0 0.0005.0 0.019 9.7 97.6 W I10 0 0.00 0.00 0.00 0.000 0.000 X 10 1 14.09 14.71 0.62 0.000 0.062 Dunnelt's NISD value: PMSD: 0.1095 14.5 MSD = Minimum Significant Difference PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the nminimsoum percent difference between the control and treatient that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound detemained by USEPA (10th percentile) 12%. Upper PMSD bound de.ermssned by USEPA (90th percentile) = 30%. Lower and upper PMSD bounds were detenm'ined from the 10th and 90th percentile, respectively, ofPMSD data from EPA's WET lnterlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 2601a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Enviromtoetntal Protection Agency, Cincinnati, OH. Organisms obtained from Aquaaox, Inc. atoxl2-04-07
cr~ 00 0 -5 0 Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Crowth and Survival Test-7 Day Survival Start Date: 12/4/2007 Test ID: PpKCICR Saraple ID: REF-RefToxicant End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Samaple Type: KCL-Potassiumn chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-P insephales promelas Conmlnents: Conc-mng'L 1 2 3 4 D-Control 1.0000 0.9000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 600 1.0000 0.9000 0.9000 0.7000 750 0.7000 0,6000 0.5000 0.6000 900 0.5000 0.4000 0.3000 0.5000 1050 0.1000 0.0000 0.0000 0.1000 Transforna: Arcsin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV% N Suit Critical Resp Number D-Control 0.9750 1.0000 1.3713 1.2490 1.4120 5.942 4 [ 40 450 1.0000 1.0256 1.4120 1.4120 1.4120 0.000 4 20.00 10.00 0 40 600 0.8750 0.8974 1.2253 0.9912 1.4120 14.199 4 13.50 10.00 5 40
- 750 0.6000 0.6154 0.8872 0.7854 0,9912 9,469 4
10.00 10.00 16 40
- 900 0.4250 0.4359 0.7088 0.5796 0.7854 13.871 4
10.00 10.00 23 40
- 1050 0.0500.
0.0513 0.2403 0.1588 0.3218 39.161 4 10.00 10.00 38 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Will/s Test indicates norosal distribution (p. > 0.01) 0.95269156 0.884 -0.5882964 0.98608425 Equality of variance cannot be confirnied Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steels Many-One Rank Test 600 750 670.820393 Treatments vs D-Control Maximusm Likelihood-Probit Parameter Value SE 95% Fiducial Lnimts Control - Chi-Sq Critical P-value Mu Sigma Iter Slope 10.5797807 1.55653174 7.52897844 13.630583 0.025 5.38853779 7.81472778 0.15 2.90749024 0.09451992 5 Intercept TSCR Point ECOI EC05 EC10 EC15 EC20 EC25 EC'o EC50 EC60 EC75 EC80 EC85 EC90 EC95 EC99 -25.760609 4.53827135 0.01416527 0.01444836 Probits mg/L 2.674 487.084184 3.355 564.961442 3.718 611.445976 3.964 644.950886 4.158 672.884107 4.326 697.810537 4.747 764.792979 5,000 808.146767 5.253 853.958158 5.674 935.929107 5,842 970.59983 6.036 1012.63712 6.282 1068.12572 6.645 1156.01018 7.326 1340.83853 -34.655621 -16.865597 -0.0141535 0.04248405 95% Fiducial Limits 388.552161 553.955155 477.157271 623.413202 531.846681 664.596996 571.834898 694.406478 605.364523 719.506485 635.283585 742.239349 714.272365 806.270707 762.708183 851.574385 810.216562 904.099344 885.93285 1009.83303 915.339908 1058.10293 949.647481 1118.70394 993.323362 1201.50749 1059.97762 1337.91255 1193.93693 1641.5599 Organisms obtained from Aquatox, Inc. atox12-04-07
ON 0 0 Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish GrowAh and Survival Test-7 Day Gn-owth Start Date: 12/4/2007 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: / Conc-mg/L 1 2 3 4 D-Control 0.8320 0.7020 0.7140 0.7740 450 0.6180 0.6320 0.7010 0.7950 600 0.4940 0.3340 0.4710 0.4330 750 0.4170 0.3470 0.2800 0.2240 900 0.2370 0.1700 0.1260 0.2650 1050 0.0120 0.0000 0.0000 0.0620 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7555 1.0000 0.7555 0.7020 0.8320 7.934 4 0.7555 1.0000 450 0.6865 0.9087 0.6865 0.6180 0.7950 11.788 4 1.374 2.180, 0.1095 0.6865. 0.9087
- 600 0.4330 0.5731 0.4330 0.3340 0.4940 16.312 4
6.422 2.180 0.1095 0.4330 0.5731 750 0.3170 0.4196 0.3170 0.2240 0.4170 26.341 4 0.3170 0.4196 900 0.1995 0.2641 0.1995 0.1260 0.2650 31.661 4 0.1995 0.2641 1050 0.0185 0.0245 0.0185 0.0000 0.0620 159.711 4 0.0185 0.0245 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96612656 0.805 0.13911643 -1.0072421 Bartlett's Test indicates equal variances (p 0.89) 0.23211719 -9.2103405 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 450 600 519.615242 0.10947132 0.14489916 0.115353 0.00504333 3.OE-04 2, 9 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95% CL(Exp) Skew IC05* 246.36 125.47 45.16 602.14 0.2837 IC1O 453.88 78.92 113.63 509.09 -0.8982 ICI5 476.23 36.47 293.20 526.96 -2.2311 IC20 498.58 21.00 433.42 550.11 -1.0351 IC25 520.93 17.71 458.63 572.23 -0.2358 IC40 587.99 .-21.77 543.80 670.46 0.6688 IC50 671.44 40.37 548.59 817.13 0.3585
- indicates IC estimate less than the lowest concentration Organisms obtained from Aquatox, Inc.
atox12-04-07
Environmental Testind Solutions, Inc. P-aoe 4 of5 Fnvironmen~al Testing Solutions, Inc. Paged of5 Species: Pimephalespromelas PpKCICR Test Number: I2 Daily Chemistrv: Day 0 12 _____Analyst V'ý mt Concentration Parameter V CONTROL pH (S..) /f,
- I___,
Q:_ DO (mg/L)
- l
/ IConductivity fV (ýimhos/cm) CD C Alkalinity (mg CaCO3/L) .i I Hardness (mg CaCO 3/L) Temperature 2 z21 (oC) __.______z .l zqq pH (S.U.) U____ -92,,- O.ii q L ') "71. L DO (muL) ,lL. hi "1 450 mg KCI/L Conductivity rem (ýimhos/ctn) i I o i 0 F Temperature (oC) pH (S.U.) Q., [ 1, o9 DO (mg/L)
- t
): }O07" 600 mg KC/L Conductivity
- 17.
(prmhos/cm) t
- Temperature
_ (oc) pH (S.U.) -. 42 LUrL ").S5 DO (mg/L) 750 mg KCI/L Conductivity Temperature 2"4zq..sN PH (S.UC ) DO (mg/L) D' 900 mg KCI/L Conductivity %-A, (°C)hoscm) No 2-4 pH (s-u) n ' 9. 9s:-. ".9 DO (C g/L) 0._] 1050 mg Conductivity KCI/L (,umhos/cm)' (AK' T e m p era tu re 2 4 S1.q S-STOCK Conductivity C0 Initial Final Initial Final Initial Final Page 87 of 101
Environmental Testing, Solutions, Inc. Page 5 of5 Species: Pimeph ales promelas PpKC1CR Test Number: Day ,3 4 5 6 Analvst 'L-C oncentration Param eter -NA 6 1i" CONTROL pH (S.U.) to a -t- .qs e DO(maL) '4 4 Conductivity A
- z*
- j}
(5C'Y, (4mhos/cm) N Alkalinity (mg CaCO3/L) S Hardness (mg CaCO3/) Temperature 2A.1 -iA. (OC) z Lkk pH (S.U.) ). %Cj* DO (rnag/L) 1.1T6 450 mg KCI/L Conductivity ( mhos/cm) l Temperature (0c) -L.k --,1.- PH (S.U.) ".q -7 ".2o 3 5.'0 DO (mg/L) (r. g KCI/L Conductivity 1-0 Sj(onthos/cm) HDC Temperature 7zq.o "L... -*.._.o .-.2. -q,. (0c) pH (S.U.) 4, 1 4, 4 ) .,cc 6ý, -1. 0 -Go-7 75 7Smg KCI/L Conductivity ( mrrhos/cmn) Temperature -L (oC) Zk. -6 "t. -Lq.g - 5 -L'q pH (S.U.) o 7 q ".'bO DO(mg* 9050 mg KCI!L Conductivity ( Mhos/cmn) o Temperature (0c) T-z .',1. a DO (ma/L) 1050 mg Conductivity Temperature 7q.] zq Z, -L.12. 1-k 7L 0 STOCK Conductivity (pimhos/cm) Initial Final Initial Final Initial Final Initial Final 1Page 88 of 101
EjivjiUJIIIIIILIIL~aI XU3LIIIg.. YUIuJLIuII, Li9. Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 1.12 1.10 .1.08 1.06 1.04 1.02 2.5 USEPA Control Limits (+/- 2 Standard Deviations) i i i i
- 1 1
1 1 1 1 1 1 1 j I III I I I I I I. I I I I I I I I I I I USEPA Warning and Control Limits (75th and 90th Percentile CVs) 2.0 H 1.5 I-1.0 H 0.5 I I I
- I I
I I I I I I I I r 2 1 A .9 I 1.3 1.2 1.1 1.0 I 'I I I I I I I I I I 1 Laboratory Warning and Control Limits (101h and 25th Percentile CVs) I 0.9 - 0.8 O'Cal. 01 AN JAI -zb ";, Ob 061 Q", CA- 0 Q$- is.1 ýabr 0 isl Z110 69 Ný.O NVO N ýA 4A Q1. 6,Y03, QIN-0ý- 03, QýAZ OQll I ýV 10 )IN IýN Test date 7-day IC2 5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean IC25) Warning Limits (mean IC25 +/- S A10 or SA.75) aontrol Limits (mean IC25 + SA.2, S or 2 Standard Deviations) pag~e 89 of [1 2 .5
0 0 0 Environmental Testing Solutions, Inc. Ceriodaph nia dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water Test nunsher Test date 7-day IC 25 CT (g/L NaCI) (g/L NaCl) State and USEPA S Control Limits SAAO CT-2S CT+2S Laboratory Warning Linfts S,5s CT - SN1o CT + SAJo Laboratory Control Limits CT - S* 5s CT + S125 USEPA SkS Warning Limits CT - SA.7s CT + S,,T USEPA Sk.9o Control Linits CT - SA,9o CT + Sss CV 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 26 06-06-06 1.06 07-11-06 1.09 08-08-06 1.07 09-12-06 1.06 10-03-06 1.06 11-07-06 1.06 12-12-06 1.09 01-09-07 1.07 02-06-07 1.09 03-06-07 1.09 04-03-07 1,10 05-08-07 1,10 06-05-07 1,10 07-10-07 1,06 08-07-07 1,10 09-05-07 1.08 10-02-07 1.06 11-06-07 1.06 11-06-07 1.07 12-04-07. 1.07 1.07 0.02 1.03 1.11 0.09 0.98 1.07 0.02 " 1.04 1.10 0.09 0.99 1.07 0.01 1.04 1.10 0.09 0.98 1.07 0.01 1.04 1.09 0.09 0.98 1.07 0.01 1.04 1.09 0.09 0.98 1.07 0.01 1.04 1.10 0.09 0.99 1.07 0.01 1.04 1.10 0.09 0.9"9 1.07 0.01 1.05 1.10 0.09 0.99 1.07 0.01 1.05 1'0 0.09 0.99 1,08 . 0.02 1.05 1.11 0.09 0.99 1,08 0.02 1.05 1.11 0.09 0.99 1,08 0.02 1.05 1.11 0.09 0.99. 1,08 0.02 1.05 1.11 . 0.09 0.99 1,08 0.02 -1.05 1.11 0.09 0.99 1.08 0.02 1.05 1.11 0.09 0.99 1,08 0.02 1.05 1.11 0.09 0.99 1.08 0.02 1.04 1.11 0.09 0.99 1,08 0.02 1.05 1.11 0.09 0.99 1,08 0.02 1.05 1.11 0.09 0.99 1.16 1.16 1.15 1.15 1.15 1.16 1.16 1.16 1.16 1.16 1.16 1.17 1.16 1.17 1.17 1.16 1.16 1.16 1.16 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.90 0.90 0.89 0.90 0.90 0.90 0.89 0.89 0.89 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25. 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.26 0.48 0.59 1.26 0.48 0.59 1.26 0.48 0.59 1.26 0.49. 0.59 1.26 '0.49 0.59 1.26 0.49 0.59 1.26 0.49 0.59 1.26 0.49 0.59 1.26 0.49 0.59 1.26 0.48 0.59 1.26 0.48 0.59 1.26' 0.48 0.59 1.55 1.55 1.55 1.55 1.55 1.55 1.55 1.56 1.56 1.56 1.56 1.57 1.56 1.57 1.57 1.56 1.56 1.56 1.56 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 1.73 0.02 1.74 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.74 0.01 1.74 0.01 1.74 0.01 1.74 0.01 1.75 0.01 1.75 0.01 1.75 0.02 1.75 0.02 1.75 0.01 1.75 0.02 1.75 0.02 1.75 0,01 1.74 0.01 Note: 7-d ICzs= 7-day 25% inhibition concentration. An.estiniation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (niean IC25)." S = Standard deviation of the IC25 values. Laboratory Control and Warning Limits Laboratory control and warning limits were established usiing the standard deviation of the IC25 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning linits recommended by USEPA for the test nmethod and enidpoint. S,.10 = Standard deviation corresponding to the 10 h percentile CV. (S~js = 0.08) SA.15 = Standard deviation corresponding to the 25t' percentile CV, (SAs5 = 0.17) USEPA Control and Warning Limits SA7s ý Standard deviation corresponding to the 75." percentile CV. (SA7s = 0.45) SA.90 = Standard deviation corresponding to the 90*' percentile CV. (SA0s = 0.62) CV Coefficient of variation of the IC25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elinsinition Program. EPA-833-R-00-003. US Enviroinetal Protection Agency, Cincimnati, OH. 12-04-07
Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Test Control Control Mean Test date CT CV CT MSD PMSD CT number Survival Reproduction tbr Control Mean for Control (%) (off'spring/female) Reproduction (%). Reproduction (%) for PMSD (%) (offspring/female) CV (%) 1 06-06-06 100 30.3 5.2 3.0 9.8 2 07-11-06 100 29.0 29.7 5.4 5.3 2.5 8.6 9.2 3 08-08-06 100 28.6 29.3 8.9 6.5 3.7 12.8 10.4 4 09-12-06 100 30.9 29.7 5.4 6.2 3.3 10.6 10.5 5 10-03-06 100 32.3 30.2 4.6 5.9 3.1 9.5 10.3 6 11-07-06 100 31.0 30.4 6.3 6.0 2.4 7.8 9.9 7 12-12-06 100 28.0 30.0 6.7 6.1 .2.2 8.0 9.6 8 01-09-07 100 27.6 29.7 4.9 5.9 2.4 8.6 9.5 9 02-06-07 100 29.9 29.7 7.6 6.1 2.9 9.8 9.5 10 03-06-07 100 30.1 29.8 6.0 6.1 2.6 8.7 9.4 11 04-03-07 100 33.1 30.1 4.4 5.9 2.2 6.6 9.2 12 05-08-07 100 32.0 30.2 8.5 6.2 2.7 8.4 9.1 13 06-05-07. 100 28.4 30.1 7.5 6.3 2.5 8.7 9.1 14 07-10-07 1.00 29.3 30.0 6.0 6.2 2.5 8.5 9.0 15 08-07-07 100 .28.7 29.9 7.5 6.3 2.9 10.0 9.1 16 09-05-07 100 29.7 29.9 5.7 6.3 2.5 8.4 9.1 17 10-02-07 100 29.5 29.9 .9.4 6.5 2.4 8.2 9.0 18 11-06-07 100 28.0 29.8 6.1 6.4 2.5 8.8 9.0 19 11-06-07 100 30.4 29.8 5.6 6.4 2.2 7.2 8.9 20 12-04-07 100 31.8 29.9 5.5 6.4 2.5 7.9 8.8 Note: CV= Coefficient' of variation for control reproduction. Lower. CV bound determined by USEPA (1 0th percentile) = 8.9%. Upper CV bound determined by USEPA (9 0 th percentile) = 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment That can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (1 0 th percentile) = 13%. Upper PMSD bound determined by USEPA (9 0 th percentile) = 47%. CT Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-82 I-B-01-004 and EPA-821-B-0.1-005. US Environmental Protection Agency, Cincinnati, OH. 12-04-07 Page 91 of 101
Environmental r esting soiutlons, inc. Ceriodaphnia dubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests 0 o ~ i~ o~ oQ 0 0 z. ~ 0 i~ 0~ 0 -~ ~ 0 ~ 00 QC~. 40 35 30 25 20 15 40 30 20 10 50 40 30 20 10 n3 I I I Minimum Acceptance Criteria (> 15.0 offspring per surviving female) S I I I I I I I I I I I-t* I I I I I ! I I 1. 1 I I 1 1 I I I I I North Carolina Acceptance Limit (< 40.0%) Kentucky Acceptance Limit (< 30.0%)2 i~~. f i I I I I I I I I I I I I I I I I I I USEPA Upper PMSD Bound (9 0 "h percentile < 47,0%) .I I I i I I I ..I .i I I U/ Test date Control Reproduction, Coefficient of.Variation (CV), or Percent Minimum-Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- Central Tendency (mean Control Reproduction, CV, or PMSD)
Page 92 of lAJlntrol Limits (mean Control Reproduction, CV, or PMSD + 2 Standard Deviations)
Environmental Testing Solutions, Inc. Page 1 of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia CdNaCLCR 4: tOk Dilution preparation information: Comments: NaC! CHM number: - (___.4 Stock preparation: 100 g NaCkIl (dissolve 50 g NaCI in 500 ml deionized water) Dilution prep (mg(L) 600 800 1000 1200 1400 Stock volume (miL) 9 12 115 I8 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500. Test organism source information: Test information: Organism age: -< 24-hours old Randomizing template color: 'eVR-A-e. Date and times organisms were born I-.. O"-r 0 t-L T-ro ItW' Incubator number and shelf between: location: Culture board:. i\\- '.fVO" Replicate number: I 2 3 1 4 5 6 7 8 YWT.batch: to-i. al Culture board cu number:
- 1. 1 Ito Lt Iq 17
'Z Transfer bowl information: pH =-. " SU Temperature= A 9C Selenastrum batch: .\\ -1 -J Daily renewal information: Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0A 2 1L-ai-cr I t-i-at-U) 026 4 1-(--0\\ I-O-t-fq.-l 5 s ,.o4-01 Control information: Acceptance criteria Summary of test endpoints: % of Male Adults: t*7,
- 5 20%
7-day LCso 'qoco % Adults having 3r dBroods: C01. - 80% NOEC M icOo % Mortality:
- 07.
- 20%
LOEC 1.0 Mean Offspring/Female: \\ 15.0 offspring/female ChV l(%A., %CV: .2 < 40.0 % I IC25 11.2. Page 93 of 101
Environmental Testing Solutions, Inc. Page 2 of 6 Environmental Testing Solutions, Inc. ?aoe2 of6 Species: Ceriodaphnia dubia CdNaCLCR #: ___ I CONTROL Survival and Reproduction Data I N N N N N N ae94o Replicate number Da 2 3 4 5 6 7 8 9 10 I Young prodced _) Adult mortality '7 Yourspoue q1 c) ~fS t 3 Young produced C CA C) C ) Adult mortality j L .i '*'-[ L._ jioug produced ~ 1' ]3~__ Adult mortality J "J - \\...._ 5 Young produced Au morta l A 0] 0 I z 10 1_2-Lol1 [ Adlmralt J J__~-~k -d--I' 6 Young produced 0 7 [ Young produced 11,o 1k \\ko %to ', -A L. k Total young produced _5 7_ 2 3 3 2.9 t z_ Final Adult Mortality X for 3rd Broods K( 1-X- j. Note. Adult mortality (L = live, D =dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over w ith adultrduring transfer)- _ _C o n c e ntr ati o n : Concentration: % Mortality: Mean OffspringFemale: 31, V" 600 mg NaCUL Survival and Reproduction Data Replicate number Da 1 2 3 4 5 6 7 8 9 10 1 Young produced c) I O 0 0 l
- l Adult mortality U
U V 2 Young producedLQ o .Adult mortality J 1 -IJ J.I't. .___ j-- I U t--I......._ Adult mortality 1 II.. T.T 3
- ~Young produced c)
ILC)~~~~~ c .L) -I -71 4 [Young produced J 4 LA~ il$ S-L L[ A Adult mortality I _I '-- II-[ igproduced (J 0~7jQ C 3 ) 2 ~ ~ 7 -Young produced l ' IS t to ILI iI q 1-1 i .- I 'S Total young produced
- 53S 3,z.
31 3" '2. 3Z 31A Final Adult Mortality I 2
- k.
i I L ca, .01 te: I.uult morality .L - U - uau), ) -b spi Uitbood 51igie ood Spuit ueLtven tWO Udays), 0L carry ovetou (Olsprlng Fried over with adult during transfer). Concentration: % Mortality: W. Mean Offspring/Female: 3Z,8 % Reduction from Control: -3. k I',
Environmental Testing Solutions, Inc. Page 3 of 6 Species: Ceriodaphnia dubia 800 mp NaCIIL CdNaCLCR #: 1\\ Survival and Reproduction Data " Replicate number Dav 1 2 3 4 5 6 7 8 9 10 I Ydung produced n 0 C Q. 0 j 2 Young prdue Adult mortality 31 Young produced C) r)L O ~ A dult m ortality 4 Young produced Adult mortality 5 Young produced I'I' 1 a t'].~1 I~l]~ I Adult mo tlt -- I k..._-II k._Ik Young produced o 01 Adult t _ortal_-ty 74.$ Young produced -I
- 1.
1 1 1 1 'Total young produced J 31 3 3' 30 .3%A -6q 21~ 3 Final Adult Mfortality \\- C K I,.. Note." Adult mortality (L = live, D = dead), SB =split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer). Concentration: % Mortality: 67. Mean Offspring/Female: 3\\ A % Reduction from Control: - 0.37. 1000 mg NaCIfL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 .Young produced C) 0 _ 0 0
- 0. o K
Adult mortality L J _._ I_ I* 2 Youngproduced Q ° c rT l0 °,°'° °, Adult mortality I L- £ -L-IL-I ---I 3 Young produced c-r Adult mortality L"] [ I -I 4 Young produced -5 s 4[-I L T LFq .3 A Adult mortality 1 .1 II .. I,..t... .. jL-5 Young produced I
- j.
) J 3T LIf i[ iI{ vzjv2.. Adult mortality I._ I L *J*tL j 6 Young produced rQ CQ Q ) 0 0 V Io 7C .Adult mortality I L-I.2 L-I I-7 Young produced LA J"!.. IZ Jr,, t l1o IA13 13 1 Z Total young produced "'.vt' -- 3\\ Z 3, 2"1 2 -* 2-8 Final Adult Mortality
- k.
-C, C Note. Adult mortality (L = live, D = dead), 31 - spi utoo' tsi n e -'o split between two days), CO = carry over (offspring carried over with adult during transfer). Concentration: % Mortality: 07. Mean Offspring/Female: 2 A.j % Reduction from Control: (d.7. a Page 95 of 101
Environmental Testing Solutions, Inc. Page '4 of,6 Species: Ceriodaphnia dubia CdNaCLCR #: icL 1200 mgo NaCIfL Survival and Reproduction Data _Replicate number Day 2 3 5 16 71 8 9 10 I Young producd __1 .C L )0 Adult mortality L L_....I 2 Young produced 1c1 c)[cJc ~ I I 1 I f Adult mortality j---_*-- I s-4 Young produced -n1_O16 ul otaiy k_ I.. Adult mortaliyj. 1 i t'I L'1 { 5 Young produced 5 -A_'A Adult mortality 7 ] IYoung produced 11 1 (0 Total young produced ia 2-1 l' 12." e s- 'N i$ 1, l3 Final Adult Mortality L h 17]...h
- t.
Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring .carried over with adult during transfer). Concentration: % Mortality: OT7. Mean Offspring/Female:
- 1. 'A
% Reduction from Control: 5S4,11. 1400 mg NaCLfL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 to I Young produced 0 0 C 0 0 C0 0 0 Adult mort~ality FE I._ 2 _ungprduced r_ Adult mortality. C L 2 Young produced 10 ]oL c jC)~~U Adult mortality 13 Young producejd ] OLA.~ [_ L 4 Young produced C._-__ Adult mortality 6 [ Young produced 1 C) 0 Adult mortality C-7 [ Young produced -7 Total young produced 2'.zo o I... 10 Final Adult M ortality I.- I- ._.Z.. xr_._ a J... - / -t., 'r-,- A o*A\\C
- I e~~l'r A ('/ ;*
a.^..-A ^--, a m* tvore: .Adult mortality kL = live, D' -e Su "t uie brood spit obeween two fays), Cu = carry over (otrspring carried over with adult during transfer). I Concentration: % Mortality: T-07. Page 96 of 101 Mean Offspring/Female: 9-!S % Reduction from Control: -70. I 1
0 0 Environmental Testing Solutions, Inc. Verification of Ceriodaphnia Reproduction Totals Control Day Re licate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 3 4 4 4 4 4 4 40 5 11 14 13 13 12 14 10 12 11 11 121 6 0 0 0 0 0 0 0 0 0 0 0 7 16 16 16 14 17 14 .15 18 16 15 157 Total 32 34 33 30 33 32 29 34 31 30 318 600 mg NaCIIL Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 5 4 4 5 4 4 43 5 13 14 14 13 11 13 11 12 12 11 124 6 0 0 0 0 0 0 0 0 0 0 0 7 19 15 16 18 14 14 17 15 I8 15 161 Total 36 33 35 35 30 31 32 32 34 30 328 800 mg NaCl/L D Re licate number Total Day 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 4 4 5 5 4 4 4 43 5 11 11 12 12 13 11 12 12 10 13 117 6 0 0 0 0 0 0 0 0 0 0 0 7 17 16 15 15 18 14 17 18 15 14 159 Total 33 31 31 31 35 30 34 34 29 31 319 1000rmg NaCIIL Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 4 5 4 4 4 4 3 4 42 5 10 10 13 12 11 11 13 10 12 12 114 6 0 0 0 0 0 0 0 0 0 0 0 7 14 17 14 13 16 14 16 13 .13 13 143 ,Total 29 32 31 30 31 29 33 27 28 29 299 1200 mg NaCI/L Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 3 3 3 4 4 4 2 4 36 5 4 7 6 2 1 2 2 2 3 3 32 6 0 0 0 0 0 0 0 0 0 0 0 7 10 9 6 7 5 8 8 9 8 6 76 Total 18 21 15 12 9 14 14 15 13 13 144 1400 mg NaCI/L Re licate number Total Day 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 2 4 3 3 1 2 4 2 29 5 3 4 .2 2 4 1 0 3 3 1 23 6 0 0 0 0 0 0 0 0 0 0 0 7 6 2 5 5 4 6
- 0 3
5 7 43 Total 13 10 9 11 11 10 1 8 12 10 95
cr~ 0 -t 0 Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: Test dates: CdNaCICR #69 December 04-11, 2007 Reveiwed by: Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (mg/L NaCI) 1 2 3 4 5 6 7 8 9 10 (0/,) (offspring/female) variation (%) control (1!.) Control 32 34 33 30 33 32 29 34 31 30 100 31.8 5.5 Not applicable 600 36 33 35 35 30 31 32 32 34 30 100 32.8 6.6 -3.1 800 33 31 31 31 35 30 34 34 29 31 100 31.9 6.2 -0.3 1000 29 32 31 30 31 29 33 27 28 29 100 29.9 6.2 6.0 1200 18 21 15 12
- 9.
14 14 .15 13 13 100 14.4 22.7 54.7 1400 13 10 9 II 11 10 1 8 12 10 100 9.5 34.8 70.1 Dunnett's MSD value: 2.528 MSD = Minimum Significant Difference PMSD: 7.9 PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10 th percentile) 13%. Upper PMSD bound determined by USEPA (901h percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 2001 a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-0l-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
-V. C 0 Environmental Testing Solutions, Inc. Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 12/4/2007 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 12/11/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mg/L 1 2 3 4 5 6 7' 8-9 10 D-Control 32.000 34.000 33.000 30.000 33.000 32.000 29.000 34.000 31.000 30.000 600 36.000 33.000 35.000 35.000 30.000 31.000 32.000 32.000 34.000 30.000 800 33.000 31.000 31.000 31.000 35.000 30.000 34.000 34.000 29.000 31.000 1000 29.000 32.000 31.000 30.000 31.000 29.000 33.000 27.000 28.000 29.000 1200 18.000 21.000 15.000 12.000 9.000 14.000 14.000 15.000 13.000 13.000 1400 13.000 10,000 9.000 11.000 11.000 10,000 1.000 8.000 12.000 10.000 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical' MSD Mean N-Mean D-Control 31.800 1.0000 31.800 29.000 34,000 5.507 10 32.300 1.0000 600 32.800 1.0314 32.800 30.000 36.000 6.555 10 -0.905 2.287 2,528 32.300 1.0000 800 31.900 1.0031 31.900 29.000 35.000 6.173 10 -0.090 2.287 2.528 31,900 0.9876 1000 29.900 0.9403 29.900 27.000 33.000 6.197 10 1.719 2.287 2.528 29.900 0.9257
- 1200 14.400 0.4528 14.400 9.000 21.000 22.728 10 15.741 2.287 2.528 14.400 0.4458
- 1400 9.500 0.2987 9.500 1.000 13.000 34.824 10 20.174 2.287 2.528 9.500 0.2941 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.66751724 1.035
-0.4971278 2.37645227 Bartlett's Test indicates equal variances (p = 0.19) 7.37160444 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 1000 1200 1095.44512 2.52762202 0.07948497 '1062.59 6.10925926 '5.3E-32 5, 54 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95% CL Skew IC05 IC10 IC 15 IC20 IC25 IC40 IC50 921.5 60.3770384 781.022523 1006.55043 -0.4592 1010.70968 13.9162817 967.482909 1025.43088 -2.1844 1031.54839 7.70863446 1015.09719 1045.89372 -0.3276 1052.3871 7.3164753 1037.01044 1065.80062 -0.2733 1073.22581 7.32317592 1058.07312 1086.73913 -0,1340 1135.74194 9.47972383 1118.60057 1155.0747 0,3823 1177.41935 12.637724 1158,20347 1208.08873 0.8224 12-04-07
Environmental Testing Solutions, Inc. Paut5,of 6 CdNaCLCR #: A Species: Ceriodaphnia dubia Dail I-h. fri Y 0 i~ay I 2 0 Analist V_ lkjRi Concentration Parameter pH (S.u.) DO (mo L) CONTROL Conductivity (pmhos/cm), Alkalinity (mg CaCO3/L) Hardness (mg CaCO3 /L) Temperature -LA _ (°C ) pH (S.U.) 77.. I 1 DO (moL) 4-5 600 mg NaCJIL Conductivity 1 (+/-mhos/cm) Temperature 7 L 7_q (0c) L___"-__ PH (S.U,) 14-__ (41(P. 1.-41 DO (mg/L)
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Page 100 of 101
mental Testing Solutions, Inc. Page 5 of 6 Enxiron mental Testing Solutions, Inc. Page 6 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: __,90 Dav 3 4 5 6 Analyst rLKVL~ Concen t ra t ion Parameter
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PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facilityv TA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form/ DISCHARGE MONITORING REPORT (DMR) OMB N (SUBRl 0M1) TN0026450 103 G F - FINAL PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND MONITORING PERIOD EFFLUENT Approved. No. 2040-0004. YEAR MO DAY YEAR I MOI DAY From
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12 01 To 1 07 12 31 NO DISCHARGE NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. !FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH 00400 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS VALUE I ~*l. I I-I- F I-r I 1 SAMPLE MEASUREMENT 7.5 8.7 12 SU 0 13/31 THREE! WEEK GRAB GRAB
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- information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 08 !01 14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVEI information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MID DAYý TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 _-INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 107 G F - FINAL PERMIT NUMBER DISCHARGE NUMBER METAL CLEANING WASTE POND MONITORING PERIOD EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard I YEAR I MO I DAY I YEAR M DAY NO DISCHARGE From i 07 12 01 Tol 07 i, i is NOTE: Read instructions before completingl this form. I.-- i PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY SAMPLE OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM
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'<"N" ******* PERMIT REPORT' REQUIREMENT. 4, KM&~AV'Gt REPORT. MGD J"DAILYN~X ,t'4 "4' LC '-4 r'AL AILCTID NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my J[ TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel {j1" L Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or ,V.,. ,,,1,"&, .S,--L. persons who manage the system, or those persons directly responsible for gathering the Pnicipal Environmental Engineer 423 843-6700 08 01 14' Sinformation, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period Preiou Formon 3320- (RE 3/99) EPA Fom 33201 (REV3/99) Previous editions may be usedPae1o1 Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 ". (INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 110 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER MONITORING PERIOD EFFLUENT Form Approved. OMB No. 2040-0004 I YEAR MOI DAY I YEARI MO I DAY From 1 07 1.12 011 To 1 07 1 12 1 31 NO DISCHARGE L NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE I EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 04*04 CENTIGRADE MEASUREMENT 04, 00010 Z 0 0 PERMT .C* Ai* DEGC 383 DGC INSTREAM MONITORING REQ-UIREMENTLI-7.. DAIL. M. .3,___, PH SAMPLE
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IFM-N 0.,,_______ __10-, ALYM SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my (l, I Iy TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engtneer information, the information submitted is, to the best of my knowledge and belief, true, 423 .843-6700 08 01 "_14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED .CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUER 01) TN0026450 L 110 T F-FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER MONITORING PERIOD EFFLUENT Y A Y-I YEAR MO DAY NO DISCHARGE From L1O0 12 To 07 I12 !31 31 Form Approved. OMB No. 2040-0004 .1; f-PARAMETER QUANTITY OR LOADING QUALITY OR CONCEN 1N4 0Ix. 1 U 1instruuciUos utnure UUrIIpoltiIIu Lsa rmUII. TRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE 1*23
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PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 TN0026450 116 G F-FINAL PERMIT NUMBER [DISCHARGE NUMBER BACKWASH MONITORING PERIOD EFFLUENT YEAR DAY ý YEAR MO IDAY0I] NO DISCHARGE From 07 1 11 TO 10 2j 31 NOTE: Read instructions before completincl this form, PARAMETER DEBRIS, FLOATING (SEVERITY) 01345 1 - 0 0 EFFLUENT GROSS VALUE OIL AND GREASE VISUAL 84066 1 0 0 EFFLUENT GROSS VALUE QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE -~ 'a-AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS SAMPLE MEASUREMENT 0 9A PASS=0 FAIL=1 0 1/31
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PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) Name TVA-- SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) - MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 117 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER BACKWASH MONITORING PERIOD EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard PARAMETER DEBRIS, FLOATING (SEVERITY) 01345 1 0 0 ýEFFLUENT GROSS VALUE OIL AND GREASE VISUAL 184066 1 0 0 iLEFFFLUENT GROSS VALUE YEAR L MO IDAY I YEAR I MO I DAY NO-DISCHARGE From F07 1-2_ -0holJ To 0 I 121! 31 !1. ODSCAG NOTE: Read instructions before completinq this form. QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. 1 FREQUENCY SAMPLE 2EX OF TTYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITSANALYSIS SAMPLE MEASUREMENT PERMIfT REQUIREMENTV 0 REPORT MO'TOTAL 9A PASS=0 FAIL=I 0 1/31 -SEE PERMIT VISUAL VISUAL-I- ~ -~ ____________ I-t I-SAMPLE MEASUREMENT PERMIT REQUIREMENT n 0 FREPORT MO TOTAL 94 YES=l NO=O 0 1/31 VISUAL VISUAL I -- SEE PERMIT SAMPLE MEASUREMENT -PERMIT REQUIREMENT I -r F t SAMPLE MEASUREMENT -- PERMIT-REQUIREMENTr SAMPLE MEASUREMENT __________________________-------I-I- -r I I PERMITl REQU)LIREMENT i-I.-------------- i -t--- -l 1 T -T-7 SAMPLE MEASUREMENT l---------- -PERMIT REQUIREMENT SAMPLE MEASUREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my .t direction or supervision in accordance with a system designed to assure that qualified personnel I e Lc. Timothy P. Cleary property gather and evaluate the-information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Envtronmental Engineer Pinformation, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete, I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page I of I
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) I TN0026450 I[118 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO* -DAY YEAR MOI DAY From l07 12 1 01 To! 07 12 31 MAJOR Form Approved. (SUBR 01) OMB No. 2040-0004 }. F - FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE NOTE: Read instructions before completincl this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MA IMUM OXYGEN, DISSOLVED (DO) 00300 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS-VALUE SOLIDS, SETTLEABLE 00545 1 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 150050 1 0 " 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT -I......................... PERMIT REQUIREMENT 2.0, DAILY MN SAMPLE MEASUREMENT PERMIT REQUIREM,.ENT 100: DAILY MVX NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS UNITS jE F TP 19 MG/L TWICE!/ GRAB 19 MG/L T
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F 4 h I SAMPLE MEASUREMENT I PERMIT. REQUIREMENT SAMPLE MEASUREMENT .I. F.... PERMITr REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Timothy P. Cleary I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Principal Environmental Engineer Site Vice President TYPED OR PRINTEDI SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1}}