ML081480421

Annual Environmental Operating Report for the Period January 1, 2007 Through December 31, 2007

ML081480421
Person / Time
Site:	Cook
Issue date:	04/29/2008
From:	Peifer M Indiana Michigan Power Co
To:	Document Control Desk, Office of Nuclear Reactor Regulation
References
AEP:NRC:8541, FOIA/PA-2010-0209
Download: ML081480421 (265)

Text

Indiana Michigan Power Company INDIAMIA Nuclear One CookGeneration Place Group MICHI*GN Bridgman, MI 49106 PWER aep.com April 29, 2008 AEP:NRC:8541 DPR-58/74 Appendix B 5.4.1 Docket Nos.: 50-315 50-316 U. S. Nuclear Regulatory Commission ATTN: Document Control Desk Mail Stop O-PI-17 Washington, D.C. 20555-0001 Donald C. Cook Nuclear Plant Units I and 2 ANNUAL ENVIRONMENTAL OPERATING REPORT The Environmental Technical Specification (ETS) 5.4.1 for Donald C. Cook Nuclear Plant (CNP)

Units I and 2 requires an Annual Environmental Operating Report be submitted to the Nuclear Regulatory. Commission (NRC) on an annual basis. Enclosed is CNP's Annual Environmental Operating Report which covers the period from January 1, 2007, through December 31, 2007, and was prepared in accordance with the requirements of ETS 5.4.1.

ETS 3.2 requires notification to the NRC of changes to the National Pollutant Discharge Elimination System (NPDES) Permit within 30 days of receipt of the change by the licensee. ETS 3.2 also requires that the NRC be notified of, and be provided a copy of, proposed changes to the NPDES Permit. By letters dated May 22, 2007, and September 25, 2007, to the Michigan Department of Environmental Quality (MI DEQ), Indiana Michigan Power (I&M), licensee for CNP Units I and 2, requested a change to CNP's NPDES Permit. By letter dated December 13, 2007, to I&M, MI DEQ issued a revised NPDES Permit for CNP.

Notification of the change to CNP's NPDES Permit, as described above, was not previously provided to the NRC as required by ETS 3.2. The enclosed Annual Environmental Operating Report provides copies of the requested changes to the NPDES Permit and copies of the draft and final change to CNP's NPDES Permit issued by MI DEQ. This letter constitutes the notification to the NRC as required by ETS 3.2. This late notification to the NRC of the change to CNP's NPDES Permit has been entered into CNP's Corrective Action Program.

U. S. Nuclear Regulatory Commission AEP:NRC:8541 Page 2 There are no new commitments in this submittal. Should you have any questions, please contact Mr.

James M. Petro, Jr., Regulatory Affairs Manager, at (269) 466-2491.

Site Vice President HLE/rdw Enclosure c: J. L. Caldwell, NRC Region III K. D. Curry, Ft. Wayne AEP, w/o enclosure J. T. King, MPSC, w/o enclosure MDEQ - WHIMD/RPS K. Yale, MI DEQ NRC Resident Inspector P. S. Tam, NRC Washington, DC

ENCLOSURE TO AEP:NRC:8541 ANNUAL ENVIRONMENTAL OPERATING REPORT

04 06 U,

Annual Environmental Operating Report January 1 2007, through December 31, 2007 Indiana Michigan Power Company Bridgman, Michigan 0

o Docket Nos. 50-315 & 50-316 6

"0 0

TABLE OF CONTENTS Page

1. Introduction 1 II. Changes to Environmental Technical Specifications 1 Ill. Non-Radiological Environmental Operating Report 1 A. Non-Routine Reports 1 B. Environmental Protection Plan 1 C. Plant Design and Operation 1 D. Environmental Monitoring - Herbicide Application 2 E. Mollusc Biofouling Monitoring Program 2 F. NPDES Applications 2 G. Special Reports 3 IV. List of Appendices Appendix Title I. Non-Routine Reports - 2007 II. Herbicide Application Report - 2007 Ill. Mollusc Biofouling Monitoring Program Report - 2007 IV. NPDES Applications - 2007 V. Special Reports - 2007

1. INTRODUCTION Technical Specifications Appendix B, Part 11,Section 5.4.1, requires that an Annual Environmental Operating Report be produced and include summaries and analyses of the results of the environmental protection activities required by Section 4.2 of the Environmental Protection Plan for the report period. The Annual Environmental Operating Report shall include a comparison with preoperational studies, operational controls (as appropriate),. previous non-radiological environmental monitoring reports, and an assessment of the observed impacts of the plant operation on the environment.

This report serves to fulfill these requirements and represents the Annual Environmental Operating Report for Units 1 and 2 of the Donald C. Cook Nuclear Plant (CNP) for the operating period from January 1, 2007, through December 31, 2007.

The following table summarizes the pertinent data concerning CNP's operation during the period from January 1, 2007, through December 31, 2007.

Parameter .Unit 1 Unit 2 Gross Electrical Generation (megawatt 9,559,146 8,389,164 hours0.0019 days <br />0.0456 hours <br />2.71164e-4 weeks <br />6.2402e-5 months <br />)

Unit Service Factor ()99.6 85.5 Unit Capacity Factor - Maximum 101.1 86.1 Dependable Capacity Net()

CHANGES TO THE ENVIRONMENTAL TECHNICAL SPECIFICATIONS There were no changes to Environmental Technical Specifications in 2007.

II. NON-RADIOLOGICAL ENVIRONMENTAL OPERATING REPORT A. Non-Routine Reports A summary of the 2007 non-routine events is located in Appendix I of this Report. No long-term, adverse environmental effects were noted.

B. Environmental Protection Plan There were no instances of noncompliance with the Environmental Protectio n Plan in 2007.

C. Plant Design and Operation During. 2007, there were no .changes in station design, operations, tests, or experiments that involved a potentially significant unreviewed environmental issue. There were no environmental evaluations performed during the reporting period.

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D. Environmental Monitoring - Herbicide Application Technical Specification Appendix B, Part II Section 4.2., requires the use of herbicides to conform to the approved use of selected herbicides as registered by the EPA and approved by State authorities. There were no preoperational herbicide studies to which comparisons could be made. Herbicide applications are managed by plant procedure PMP-2160-HER-001, Guidelines for the Application of Approved Herbicides.

A summary of the 2007 herbicide application is contained in Appendix II of this report. Based on observations, there were no negative impacts or evidence of trends toward irreversible change to the environment as a result of the herbicide applications. Based on our review of application records and field observations, the applications conformed to Environmental Protection Agency and State requirements for the approved use of herbicide.

E. Mollusc Biofouling Monitoring Program Macrofouling monitoring and control activities during 2007 are discussed in Appendix III of this report.

F. NPDES Applications Groundwater On March 8, 2007, the Michigan Department of Environmental Quality (MI DEQ) issued a modified groundwater discharge authorization on permit GW1810102. The changes included Process Wastewater being added to Seepage Basins Location and Sanitary Sewage Wastewater being added to Rapid Infiltration Basins on page 6 of the permit.

Surface Water On May 22, 2007, the plant requested a change to its NPDES Permit (MI0005827) that would remove the entire text of Part I.A.10. This request was a result of the U. S. EPA's letter, dated March 10, 2007, notifying the Regional Administrators and delegated states of the suspension of Section 316(b),

Phase II rule. A second letter, dated September 25, 2007, requested modification of CNP's NPDES Permit by changing the language in Part I.A.10 to be in accordance with the suspension of the Phase II rules.

On October 4, 2007, the MI DEQ issued CNP's draft NPDES Permit and public notice that included revisions that reflected the U.S. EPA suspension of the Section 316(b), Phase II rules in Part I.A..11 of the permit; and Deicing Discharge Authorization, Outfall 003 in Part I.A.9. of the permit. CNP's final permit was issued by the MI DEQ on December 13, 2007, with the above revisions.

The above mentioned 2007 Groundwater and Surface Water permit application correspondence is included in Appendix IV of this report.

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G. Special Reports On June 8, 2007, an interim report was submitted to the U.S. EPA, Office of Chemical Emergency Preparedness and Prevention in Chicago, IL. The report was submitted in accordance with paragraph 22 of the Consent Agreement and Final Order (CAFO) to address the status of the supplemental environmental project (SEP) and associated costs to upgrade CNP's sodium hypochlorite system. The new double-walled feed piping for the plant sodium hypochlorite system, improved leak detection, and a new sodium hypochlorite pump room with improved secondary containment remains in operation since its installation on May 11, 2006. The actual costs incurred for purchasing and installing the SEP were $730,827.10. To date, the costs incurred for purchase, installation, operation, and maintenance of the SEP are $848,427.68. These SEP costs exceed the minimum amount ($66,000) required by Paragraph 17 of the CAFO.

In 2005, CNP contracted with Normandeau and Associates to perform a Section 316(b), Phase II, Fish Impingement Mortality and Entrainment Characterization Study. The study results are presented in Appendix V of this report. It includes the impingement and entrainment monitoring program and nearfield sampling program results. Impingement mortality and entrainment estimates were made for the study period assuming the impingement morality and entrainment rates were proportional to intake flow and the plant used the design intake flow 100% of the year. Results from the nearfield sampling are discussed to characterize the fish community in the vicinity of CNP's intake structures.

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APPENDIX I NON-ROUTINE REPORTS 2007

2007 Non-Routine Reports January 3, 2007 - Notice was made to the MI DEQ that during a review of our steam generator draining process, we have evaluated one of our processes to increase our outage efficiency by shortening drainage time. The present steam generator maximum drain rate is about 40 gpm.

The plant proposed to increase the maximum drain rate to 100 gpm thereby increasing Carbohydrazide, Hydrazine, and ETA concentrations in Outfall 001 and 002 by a factor of 2.5. In a reply dated July 26, 2007, the MI DEQ determined that under the proposed conditions, Hydrazine concentrations would exceed monthly average water quality-based effluent limit (WQBEL) concentrations and therefore was not approved; but Carbohydrazide and ETA would remain below WQBEL concentrations and was approved. Since the steam generator effluent contains all three chemicals, the plant elected not to increase the maximum drain rate from 40 gpm to 100 gpm.

March 16, 2007 - On March 8, 2007, at 1618 hours0.0187 days <br />0.449 hours <br />0.00268 weeks <br />6.15649e-4 months <br />, the Plant's Environmental Department was notified that a sample pump containing a small amount of oil was lost in the Unit 2 discharge piping. Approximately 0.17 liters of seal oil was contained in the pump assembly. The pump could eventually be damaged by the high velocity flow and the tortuous path of the discharge to a point where the oil would be released to the environment. Plant personnel performed a visual inspection of the plant's discharges and the beach in the vicinity of the Unit 2 discharge. No sheen or oil was observed. It was concluded that due to the small volume of oil, this event did not pose a threat to the environment, public health, or safety. Notice of this event was made to the NRC in a separate letter dated March 19, 2007.

April 27, 2007 - Notice was made to the MI DEQ informing them of our intent to place the supplemental cooling system in service that was initially requested in a letter dated August 18, 2006. The predicted rise in Lake Michigan temperature had caused us to install supplemental cooling for both units' containment buildings. This supplemental cooling successfully reduced ambient temperatures below technical specification limits of 120 degrees Fahrenheit in the 2006 operating year. The supplemental cooling system consists of pumps drawing Lake Michigan water from the North Intake Tunnel of the Circulating Water System. The untreated lake water is strained and cooled by portable air conditioning units before entering the containment ventilation units. The supplement cooling water enters the Non Essential Service Water (NESW) system and is routed to the normal NESW discharge point. No chemicals are added to this system and the total volumes for NESW discharge remained unchanged. The system did not result in new or increased loadings of pollutants to the receiving waters of the State, and was deemed not reportable under Part II.C.10, "Notification of Change in Discharge". The system was installed on June 1, 2007, and removed from site on November 9, 2007.

June 5, 2007 - On June 1, 2007, at 1230 hours0.0142 days <br />0.342 hours <br />0.00203 weeks <br />4.68015e-4 months <br />, a plume was observed from Stormwater Outfall 002S. The plume was approximately 300' X 6' in size in Lake Michigan. The lake conditions were calm which prevented the plume from mixing. The source of the plume was from cooling system flushing operations. The flush water had mixed with the surrounding gravel and created turbid water that overflowed the filter fence area into a storm drain that was not protected by filter fabric. The discharge to the lake contained soil particles thereby increasing its turbidity and creating the plume. The cause of the failure was due to excessive flush. water flow rates and clogged filter fabric on the drains. The flushing operations were stopped until remedial measures could be made to the flushing/filter system. Notice of this event was made to the NRC in a separate letter dated June 13, 2007.

June 20, 2007 - On June 11, 2007, at 0130 hours0.0015 days <br />0.0361 hours <br />2.149471e-4 weeks <br />4.9465e-5 months <br />, the Environmental Department was notified that a diesel driven water pump had leaked a small amount of seal oil into the plant discharge piping via the supplemental containment cooling system, which discharges to Outfall 001 and 002. Approximately 1.5 gallons of seal oil was lost through a failed pump seal to the discharge side of the pump. Due to the small volume of oil, the spill was dispersed. No sheen was noted 1

during a visual observation of the discharge performed during the morning of June 11, 2007. This spill did not pose a threat to the environment, public health, or safety. The pump was removed from service and replaced with a new unit. Notice of this event was made to the NRC in a separate letter dated July 2, 2007.

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APPENDIX II HERBICIDE APPLICATION REPORT 2007

SINDIANA fff(MIGAN

-, 7iY A unit of American Electric Power Date March 19, 2008 Subject 2007 Herbicide Spray Report - Cook Nuclear Plant From Steve Hitt To Jon Harner, Environmental Manager The following herbicides were applied per manufacturers' direction by, certified Michigan licensed applicators on Cook Nuclear Plant property during 2007:

Via Contractor Via AEP Personnel Spike 80 DF/Dow Round-Up Pro Glyphosate/Du Pont Pro-Scape 19-2-9 with Milestone VM/Dow Confront DeAngelo Brothers Applications:

DeAngelo Brothers; a Michigan licensed herbicide applicator on contract to the AEP Energy Delivery and Customer Relations performed the applications (Bill Rahmi).

On the dates of June 26 and 27 of 2007 a mixture of Spike 80 DF, Glyphosate, and Milestone VM was used for total plant control in the 69 KV and 765 KV switch yards (345 KV yard not sprayed), railroad (right-of-way) tracks, switches, derails & train bridge. The rail areas were applied with a spray pattern corresponding to railroad specifications, generally 16' to 20' wide pattern from a hi-railer where grass was present along railroad. Any ditches along the right-of-way were checked for water and spray pattern decreased in these areas. A total of 38.3 pounds of Spike 80 DF, 96.2 ounces of Milestone VM, and 19.0 quarts of Glyphosate were used for the application and spread over 23.8 acres in accordance with the manufacturers' labels.

Product Name Quantity Quantity Quantity Used Used/Acre Allowed/Acre Spike 80 DF' 38.3 lbs. 1.6 lbs 2.0 lbs Milestone VM 96.2 oz 4.0 oz 5.0 oz Glyphosate 19.0 qt 0.8 qt 1.0 qt Maintenance Building and Grounds:

Round-Up Pro mixed with water in a sprayer was applied to Owner Controlled Areas by licensed applicators from the Maintenance Building and Grounds crew (L. Everett Hartwig Sr. and John Mock).

Weeds were sprayed at the Training Center Parking Lot (including around chiller units),

the sidewalk from the :Training Center to North Access, the fence along the East side of the Protected Area from North Access to South Access, the backside of TSOC (length of

building x 10' wide) stone areas inside the Protected Area, and both Sanitary Absorption Ponds. A total of 720 ounces of Round-Up Pro were used for spraying in 2007.

According to the product label, spraying should contain a 5 - 10% solution and a total permitted concentration of 40 gallons per acre. A total of 6 gallons of solution were used to treat 1.5 acres (total of 60 gallons of 10% mixed solution used).

ProScape fertilizer with Confront herbicide was applied on May 16, 2007 to all grassy areas in the Protected Area and along the main plant road near the plant entrance.

Approximately six and one-half (-6.5), forty pound bags (258 lbs'.) were applied to approximately 2 acres (-86,000 ft2) via the #14 setting on an Earthway Rotary Spreader as recommended on the manufacturer's label. The herbicide was applied by a licensed applicator (L. Everett Hartwig) from the Maintenance Buildings and Grounds crew.

The following table details the application rates used for weed control in the grass and garden beds compared to the allowable application rates.

Product Name Quantity Concentration Used Concentration Used Allowed 10.0% solution - 6 gallons of solution 40 gallons per Round-Up Pro 720 oz for 60 gallons used. acre, 1.5 acres treated.

Pro-Scape with 258 lbs. -6.5 bags/2 acres applied by #14 3.6 lbs/1000 ft2 =

Confront setting on Earthway Rotary Spreader 154.5 lbs/acre Mortality Inspection:

On December 4, 20, 26 and 27, 2007, those portions of the owner controlled area where herbicides were applied in 2007 were surveyed. There was no evidence of spillage, overspray or excessive application. No adverse environmental effects were noted during the inspection. Herbicides were applied in accordance with manufacturer's label instructions and Federal and State requirements by Michigan-certified applicators.

Preparation and application descriptions were documented on PMP-2160-HER-001 Data Sheet 1 forms. Herbicides used by DeAngelo Brothers were Spike 8ODF, Milestone VM, and Glyphosate. Sun States' licensed applicator applied Roundup Pro and Pro-Scape 19-2-9 with Confront.

DeAngelo Brothers treated areas kept void of vegetation for safety and/or security reasons. These areas were in the 69kV and 765kV Yards, and railroad tracks to Red Arrow Highway. Herbicide control was successful overall with typically 85-95% of the weeds eradicated. There was some plant growth inside the north fence of the 69kV Yard.

The flower beds, stone areas, and lawns treated by Sun States had few weeds except for broadleaves in areas excavated for Oil/Water Separator isolation valve installation and the CD EDG fuel oil unloading stone area. Other areas where the spraying was not as effective include the inside fenced area around 2-TR201AB and around U2 Main Transformers. Also, numerous weeds were observed in the grass area south of North Guard House, west of the Search Portal. The stone, mulch, microwave zones and 345 kV Yard were treated by Sun States to eliminate vegetation; these treatments were very successful.

Summary:

In summary, based upon our review of the application records, manufacturer specifications, material safety data sheets (MSDSs) and observations of the treated areas, the herbicides were applied according to the manufacturer's labeled instructions and according to Federal and State requirements. All personnel performing herbicide applications were licensed by the State of Michigan. A map has been included with this report indicating areas of herbicide application. A detailed map and application records are filed in accordance with PMP-2160-HER-00 1, Guidelines for the Application of Approved Herbicides. No signs of over spray or spillage were observed. No. adverse environmental effects occurred.

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- --T-Information I PMEP-2160-HER- 01 Rev. I Page 9_ of 14 Guidelines for the Application of Approved Herbicides Map 1 Page.

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I APPENDIX III MOLLUSC BIOFOULING MONITORING PROGRAM REPORT 2007

Mollusc Biofouling Monitoring Program 2007 Performed at Donald C. Cook Nuclear Plant Performed and Submitted By Cook Plant Environmental

Prepared for:

American Electric Power Donald C. Cook Nuclear Plant One Cook Place Bridgman, Michigan MOLLUSC BIOFOULING MONITORING PROGRAM 2007 March 2008 Cook Nuclear Plant Environmental Section

Table of Contents Page #

List of Tables and Figures Executive Summary 1 Chapter 1 -Introduction 3 1.1 Past History 3 1.2 Objectives 4 Chapter 2 -Methods 5 2.1 Whole-Water Sampling 5 2.2 Artificial Substrates 7 2.2.1 Intake Forebay 7 2.2.2 Service Water Systems 8 2.2.3 Artificial Substrate Cumulative Sample 8 Analysis Chapter 3 -Results and Discussion 10 3.1 Whole-Water Sampling 10

.3.2 Artificial Substrate Sampling, Biocide Treatment, and 14 Mechanical Cleaning 3.2.1 Circulating Water System Artificial Substrate 14 Sampling 3.2.2 Service Water Systems and Miscellaneous 15 Se'aling and Cooling Water System Artificial Substrate Sampling 3.2.3 Biocide Treatment 19 3.2.4 Mechanical Cleaning 19 Chapter 4 Summary and Recommendations 22 4.1 Summary 22 4.2 Recommendations 23 References 25 Appendix Table 1 SWS Chlorination Values for 2007 Zebra 27 Mussel Monitoring Program

List of Tables and Figures Table # Title Page #

2-1 Sampling Schedule for Zebra Mussel Monitoring at 6 the D.C. Cook Nuclear Plant in 2007 3-1 Whole-Water Sampling Program Number of Zebra 11 Mussel Veligers Per Cubic Meter, Veliger Size Range, and Mean Veliger Size (um) Collected in the D.C. Cook Nuclear Plant Forebay in 2007 3-2 Density, Average Size, and Size Range of Settled 17 Zebra Mussel Postveligers Collected on Cumulative Artificial Substrates Placed in the Forebay, in the Service Water Systems and the Miscellaneous Sealing, and Cooling Water System in the D. C. Cook Nuclear Plant in 2007 Figure #

3-1 2007 D.C. Cook Plant-Whole-Water Zebra Mussel 12 Veliger Density and Water Column Temperature in Intake Forebay 3-2 2007 D.C. Cook Plant-Whole-Water Zebra Mussel 18 Veliger Density and Zebra Mussel Postveliger Cumulative Settlement in the Service Water Systems 3-3 Screenhouse Intake Forebay 21 i

Executive Summary Biofouling studies have been conducted at the Donald C. Cook Nuclear Plant since 1983.

In 1991, monitoring of zebra mussels in the circulating water, essential service water (ESW), and nonessential service water (NESW) systems was added to the program. The objectives of this monitoring program are to detect the presence and determine the density of zebra mussel veligers in the Circulating Water System and postveliger settlement and growth rate in the forebay and service water systems, and to determine the effectiveness of oxidizing and non-oxidizing biocides in the plant systems by comparing densities and sizes of settled zebra mussels when applicable.

Veligers were present in the forebay from 26 April through 5 December 2007. Peak densities occurred on 7 June, 20 July, 30 August, and 11 October, with the major peak occurring on 20 July (196,000 veligers per cubic meter). Past years' studies have determined that zebra mussel density is independent of the volume of water entering the plant, as the concentration of veligers in the water remains the same regardless of the flow rate through the plant. The past seventeen years data suggest that the zebra mussel population is highly variable and future populations of zebra mussels are difficult to accurately predict.

Cumulative settlement was monitored in the forebay using a six-inch PVC pipe. As in 2006, the time period of collection was made to more accurately coincide with the annual fall intake crib cleaning to estimate the size and density of mussels the divers might encounter at the time of cleaning. The PVC pipe was deployed on 15 September 2006 and was retrieved on 13 September 2007. The settlement density and average size of postveligers for the 12-month period was 129,425 individuals/m 2 and 3,328u (3.3 mm).

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For comparison, the sample substrate that was pulled in 2006 had a density of 303,801 individuals/m 2 and average size of 2,470u (2.5 mm). An explanation for this difference could be attributed to a large peak veliger density (455,000 ind./M 3 ) occurring in the Fall of 2005 that was more than twice the peak veliger density (191,750 ind./m 3 ) occurring in the Fall of 2006.

Service Water Systems and Miscellaneous Sealing and Cooling Water The return sides (after systems' use) of the ESW and NESW systems and the MSCW system were monitored in the 2007 Mollusc Biofouling Monitoring Program. The results indicate that the chlorination system was effective in preventing growth and prolonged settlement of postveligers in the service water systems. Zebra mussel settlement on the service water and MSCW artificial substrates became elevated when chlorination was secured during the Unit 2 C17 Refueling Outage. Settlement was again brought under control when chlorination was resumed near the end of the outage. The results showed that even when the system was taken out of service for short periods of time for system maintenance, or that system TRC residuals fell below their target band of 0.08-0.6 ppm, settlement control was quickly re-established.

Biocide Treatment There were no biocide treatments in 2007.

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Chapter 1 Introduction 1.1 Past History American Electric Power Company (AEP) has been conducting zebra mussel monitoring studies at the Donald C. Cook Nuclear Plant since 1991. The purpose of these studies is to monitor zebra mussel veliger and postveliger settlement densities in the Circulating Water, Essential Service Water (ESW), Nonessential Service Water (NESW), and Miscellaneous Sealing and Cooling Water (MSCW) systems to help determine the effectiveness of the zebra mussel control program.

Since 1999, Grand Analysis conducted the monitoring program, designed to detect the timing of spawning and settling of zebra mussels at the Cook Nuclear Plant. In 2004 the program was taken "in house" by the Plant's Environmental staff. The program also determines densities for: 1) whole water samples for planktonic veligers; and 2) artificial substrates set within the ESW, NESW, and MSCW systems for cumulative postveliger settlement. In the Circulating Water System, a section of PVC piping was used to determine the cumulative settlement in the intake forebay.

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1.2 Objectives Specific objectives for the 2007 Mollusc Biofouling Monitoring Program were as follows:

- Conduct whole-water sampling of the Circulating Water System weekly (July-September), bimonthly (May, June, October & November), and monthly (April and December) to determine the presence and density of larval zebra mussels.

- Deploy artificial substrates (microscope slides in test tube racks) in the service water systems to determine cumulative settlement of postveligers. Collect samples monthly from May through December.

- Deploy a PVC piping section, also as an artificial substrate, in the intake forebay to determine cumulative settlement for approximately one year.

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Chapter 2 Methods 2.1 Whole-Water Sampling Whole-water sampling of the Circulating Water System was conducted from 26 April to 5 December 2007 (Table 2-1). Samples were collected from mid-depth in the intake forebay by pumping lake water through an in-line flowmeter into a plankton net. The sampling location was consistent with that of previous studies. Two replicates (2,000 liters each) were collected during each sampling date.

A Myers Model 2JF-51-8 pump or equivalent was connected to an in-line flowmeter assembly (Signet Model #P58640) and pumped water into a plankton net for approximately one hour. To minimize organism abrasion, measured flow was directed into a No. 20 plankton net that was suspended in a partially filled 55-gallon plastic barrel.

Samples were gently washed into the cod-end bucket of the plankton net using filtered Circulating Water System water and then transferred to a one-liter plastic container.

Filtered water was added to the container to ensure that a full liter was analyzed. The two samples were analyzed in an on-site laboratory.

Samples were mixed thoroughly for three minutes using a magnetic stir plate. Then, using a calibrated Pasteur pipette, a 1-milliliter aliquot of mixed sample was placed into a Sedgewick-Rafter cell for counting. An Olympus SZ-1 145 binocular microscope (18-11 Ox) equipped with cross-polarizing filters was used. Ten aliquots 5

TABLE 2-1 SAMPLING SCHEDULE FOR ZEBRA MUSSEL MONITORING AT THE D.C. COOK NUCLEAR PLANT IN 2007 Date Whole Water Artificial Substrates April 26 X(1)

May 10 X 31 X X(3)

June 7 *X 21 X 28 X July 6 X 12 X 20 X 26 X X August 2 X 9 X 16 X 23 X X 30 X September 6 X 13 X X(2) 19 X 28 X October 11 X X 25 X November 8 X X 29 X December 5 X X

1. Deploy slide racks.

2. Retrieve PVC pipe section. Read, clean & re-deploy.

3. No flow through bio-box 6

were counted and the average was extrapolated to determine the number of individuals per cubic meter. The density was calculated as follows:

Density (#/m3)=(average #*DF)/0.001 L*1 L/2000L*1 00O0Lm3 DF- Dilution Factor This process was repeated for the second replicate and the mean of the two values was calculated to yield a final density value. Size measurements were recorded for up to 50 organisms from each sample. Veliger size was measured using an ocular micrometer that was calibrated to a stage micrometer.

2.2 Artificial Substrates To determine zebra mussel settlement in the Circulating Water, a PVC section was deployed in the intake forebay, upstream of the trash racks. Bio-box side-stream samplers were installed on the return sides of both service water systems and on the Miscellaneous Sealing and Cooling Water System to determine settlement in these systems. The side-stream samplers consisted of modified test-tube racks designed to hold microscope slides and placed in bio-boxes for cumulative sampling.

2.2.1 Intake Forebay On 13 September 2007, a PVC pipe section was analyzed that was placed in the forebay on 15 September 2006. The PVC section measured 6 inches long and had an inside diameter of 3.5 inches. It had been cut in half lengthwise, rejoined using hose clamps, attached to a rope weighted by a stainless steel pipe section, and suspended at 7

mid-depth in the intake forebay. The PVC sampler was analyzed for densities and shell sizes by analyzing scrapings from two separate one-inch square sections of the PVC sampler. The PVC sampler was designed to provide information on zebra mussel accumulated infestation and sizes occurring over a 1-year period from September 2006 to September 2007.

2.2.2 Service Water Systems Side-stream bio-boxes were placed on the return side of the service water systems (1 ESW, 2 ESW, NESW) and the Miscellaneous Sealing and Cooling (MSCW) Water System. Each bio-box contained two modified test tube racks containing a total of 80 microscope slides. The racks held the slides above the bio-box base that allowed silt and sediment to fall out before they could affect the slide settlement. The bio-boxes were covered with a plant-approved fireproof fabric to limit light exposure. Plant personnel checked the bio-boxes periodically to ensure that adequate flow was available, and flow was adjusted as necessary. Ten slides from each location were retrieved monthly and immediately analyzed for densities and shell size.

2.2.3 Artificial Substrate Cumulative Sample Analysis An Olympus SZ-1 145 binocular microscope (18-11 Ox) equipped with cross polarizing filters was used for analyzing samples. After one side of the slide was scraped clean, the slide was placed on the microscope stage so that the attached postveligers could be counted. When slides became heavily infested, a sub-sampling technique was followed:

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The slides were sub-sampled using a straight edge that permitted either half or a quarter of the slide to be counted. Counts were then proportionally extrapolated to one square meter.

Settlement rates were computed by taking the average number of mussels from the ten slides and multiplying this value by 533.33 to obtain the density of zebra mussels per square meter. (One postveliger/microscope slide equals 533.33 postveligers per square meter.)

Shell diameters were measured for up to 50 random individuals to obtain maximum, minimum and mean sizes. Diameters were measured using an ocular micrometer calibrated to a stage micrometer.

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Chapter 3 Results and Discussion The zebra mussel monitoring system performed up to expectations in 2007. The whole-water sampling for free-swimming veligers coupled with monitoring postveliger settlement on artificial substrates provided sample results that could be compared with previous years' data.

Appendix Table 1 shows the chlorination values for the ESW and NESW systems. A 0.08-0.6 ppm total residual chlorine (TRC) was the target band for the control of zebra mussel settlement. Total residual chlorine values for the ESW and NESW systems were taken periodically. The MSCW system, which was cross-connected to the NESW system, was chlorinated on all of the dates that the NESW system was chlorinated.

3.1 Whole-Water Sampling Sampling of planktonic veligers in the circulating water system was initiated 26 April and was completed on 5 December. Results are presented in Table 3-1 and in Figure 3-1.

Veligers were present in all samples throughout the monitoring season.

Heaviest spawning activity occurred during late-May to early June, mid-July, late August, and mid to late October. The major peak density occurred on 20 July (196,000 ind./M 3).

This major peak occurred three months earlier than 2006's and was not matched in timing by any of the previous years' sampling. Overall, 2007's Whole-Water 10

TABLE 3-1 Whole-Water Sampling Program Number of Zebra Mussel Veligers Per Cubic Meter, Veliger Size Range, and Mean Veliger Size (um) Collected in The D.C. Cook Nuclear Plant Forebay in 2007 ,

Density Size Range Mean Size Date (No./m3) (urn) (urn) 4/26/07 5475 83-133 107 5/10/07 8525 100-150 118 5/31/07 25550 100-233 140 6/7/07 64000 100-233 157 6/21/76 2825 100-233 139 7/6/07 3350 100-300 179 7/12/07 68500 100-266 152 7/20/07 196000 83-200 134 7/26/07 19800 100-333 169 8/2/07 7425 117-433 202 8/09/07 9675 100-366 209 8/16/07 10825 117-300 205 8/23/07 6875 100-333 227 8/30/07 41650 83-283 113 9/6/07 5525 100-500 180 9/13/07 10200 67-333 118 9/19/07 1425 100-333 150 9/28/07 12650 83-466 149 10/11/07 84250 78-412 173 10/25/07 52500 83-433 125 11/8/07 9475 83-333 134 11/29/07 26325 100-466 189 12/5/07 8600 100-300 142 11

FIGURE 3-1 2007 D.C. Cook Plant- Whole-Water Zebra Mussel Veliger Density and Water Column Temperature in Intake Forebay 24 80 22 75 o 20 20 70

• - 18 x

65 Q-E 60 E

.2 14 I-C..)

55 _u 12 -o Cu 0

a1) 10 50 a)

) 8 45 -

• 6 40 35 2

0 30

- N*- N- _- - l- N,- Nl- N- N- rN- r- N- N- ,- N- N,- N- N-- N- N- N- 1-oR !R 0 0) 0 0 0 0D 0 0 0 0) 0 0 0 0) 0 0 0) 0 0D 0 (0 0 N- ( N 0 C n c 0 a )

CN

, ~U -

N CD - ( ~ 0 ' N(

N 04( -

S U ( (0 N - - N- N-o o co co co ( o 0 o 0 o C00 0 0 0 0 00 0D0 0D 0 0 0 0a Sample Date 12

peak density was much the same as 2006's (191,750 ind./m3 ) but occurring three months earlier. The recorded peak density for 2007 (196,000 ind./m 3) was less than half of those in recent years 2005 (455,000 ind./m 3) and 2003 (450,000 ind./M 3).

Secondary peaks were recorded in 2007 on 7 June (64,000 ind./m 3), 30 August (41,650 ind./M 3) and 11 October (84,250 ind./M 3).

Whole water veliger densities declined after their peak on 11 October with falling lake temperatures into December. The whole-water densities show that there are substantial numbers of veligers in the forebay, indicating the need for effective chlorination in the service water systems.

The 2003 report concluded that yearly results in peak abundances make it difficult to predict when the peak abundance will occur each season other than estimating some time between July and October. Continued whole-water monitoring during the veliger spawning season will detect when these peak abundances occur.

Whole-water densities recorded during 1993 through 1995 for the November and December sampling periods were less than 1,000 ind. /M 3 for sampling conducted after 3 November. During the 2005 through 2007 sampling seasons, whole-water densities recorded in November were about fifty times greater than those of the 1993 through 1995 period, showing that spawning is occurring late into the fall due to warm fall weather, and the larger abundance of mussels in Lake Michigan. Because of the late fall spawning in recent years, there is a need for chlorination to continue into the late fall months to prevent zebra mussel settlement and growth in plant systems.

13

In summary, zebra mussel veligers were present in the water column on all sampling dates from 26 April through 5 December. Spawning commenced in late April and continued through the end of the sampling program. Peak veliger densities occurred on 7 June, 20 July, 30 August and 11 October.

3.2 Artificial Substrate Sampling, Biocide Treatment, and Mechanical Cleaning 3.2.1 Circulating Water System Artificial Substrate Sampling Cumulative settlement was monitored in the intake forebay using a six-inch PVC pipe with a 3.5 inch inside diameter. The PVC pipe was set in the forebay on 15 September 2006 and retrieved on 13 September 2007 to determine the average density and size range for 12 months. The density on the substrate was 129,425 ind./m 2 . Individuals ranged from 193u-15,408u (.19 mm - 15.41 mm) and the mean size of fifty randomly selected individuals was 3,328u (3.3 mm). As in 2006, the time period of collection was designed to coincide with the annual fall intake crib cleaning to estimate the size and density of mussels the divers might encounter at the time of cleaning. For comparison, 2

the sample substrate that was pulled in 2006 had a density of 303,801 individuals/m and an average size of 2,470u (2.5 mm). An explanation for this difference could be attributed to a large peak veliger density (455,000 d/ind./M3 ) occurring in the Fall of 2005 that was more than twice the peak veliger density (191,750 ind./m 3 ) occurring in the Fall of 2006.

14

3.2.2 Service Water Systems and Miscellaneous Sealing and Cooling Water System Artificial Substrate Sampling The return sides (after systems' use) of the ESW and NESW systems and the MSCW system were monitored in the 2007 Mollusc Biofouling Monitoring Program. Chlorine is injected beneath each ESW pump suction. The ESW trains are typically cross-tied downstream of the chlorine injection point so that both ESW trains are served. A separate chlorine injection point, which is in the suction header, serves the NESW system and subsequently the MSCW system.

The plant's Zebra Mussel Monitoring & Control Program calls for continuous chlorination at 0.08-0.6 ppm total residual chlorine (TRC) of the service water and MSCW systems from May through November to correspond with the zebra mussel spawning season.

Cumulative settlement sampling and analysis was performed on a monthly basis in 2007. Artificial substrate slides were installed on 26 April and ten slides per month were examined and not replaced. The May sample was not taken due to a procedure needing to be developed prior to manipulating the system sample valves; thus flow was not established to the bio-boxes until mid-June. Results are shown in Table 3-2 and Figure 3-2.

The chlorine residual and postveliger settlement data indicate that the permanent chlorination system installed under 12-MOD-50719 on 18 July 2005 performed up to expectations. When chlorine was being delivered to the service water and MSCW systems, post-veliger settlement was maintained under control (<-1,000 ind./m 2 ).

From 1 May until the chlorination system was shutdown on 14 September chlorination 15

system outages were infrequent and chlorine residuals were generally maintained in the target band of 0.08 - 0.6 ppm. The elevated reading on the MSCW sample of 26 July (3,680 ind./m 2 ) could be attributed to a reduced flow to the MSCW bio-box on this date as the chlorine residuals for U1 and U2 NESW were 0.34 and 0.36 ppm respectively; well within the chlorination target band. The elevated reading on the UI ESW sample of 13 September could also be attributed to the reduced flow to the Ul ESW bio-box on this date as the chlorine residuals were 0.36 ppm; well within the chlorination target band.

Zebra mussel settlement on the service water and MSCW artificial substrates became elevated when chlorination was secured on 14 September for the Unit 2 C17 Refueling Outage. Settlement was again brought under control when chlorination was resumed on 31 October near the end of the outage. The results showed that even when the system was taken out of service for short periods of time for system maintenance, or that system TRC residuals fell below their target band of 0.08-0.6 ppm, settlement control was quickly re-established.

16

TABLE 3-2 Density, Average Size, and Size Range of Settled Zebra Mussel Postveligers Collected on Cumulative Artificial Substrates Placed in the Forebay, in the Service Water Systems and Miscellaneous Sealing and Cooling Water System in the D.C. Cook Nuclear Plant in 2007.

Cumulative Samples Forebay NESW MS&CW I ESW 2 ESW Avg. Avg. Avg. Avg. Avg.

Density Size Range Density Size Range Density Size Range Density Size Range Density Size Range Date (no/m2) (urn) (urn) (no/m2) (urn) (urn) (no/m2) (urn) (urn) (no/m2) (urn) (urn) (no/m2) (urn) (urn) 6/28/2007 1067 136 78-274 747 119 78-196 373 146 98-235 960 117 78-235 7/26/2007 480 133 78-353 3680 128 78-353 533 123 78-274 640 134 78-235 8/23/2007 213 294 235-431 480 222 98-353 480 235 98-353 320 196 98-274 193-9/13/2007 129,425 3328 15,408 320 265 118-588 800 303 118-470 3253 440 274-823 53 431 431-431 157- 274-10/11/2007 4747 448 1058 7520 655 1294 1173 266 157-627 960 272 157-627 196- 196-1118/2007 10,453 555 1646 2773 803 3606 107 196 118-274 213 333 235-470 12/5/2007 533 423 235-784 747 336 235-392 213 294 196-353 0 - -

17

FIGURE 3-2 2007 D.C. Cook Plant- Whole-Water Zebra Mussel Veliger Density and Zebra Mussel Postveliger Cumulative Settlement in the Service Water Systems 12 19.60 _.20.0

-- I ESW Density

--- 2 ESW Density 10.45 18.0 10 w 2 NESW Density 16.0

-x-- MSCW Density WW Densty 14.0 0 a 7.52 OD 2: 6 -I10.0 I0 0 4 (I) 3.25.60 2

/ 0.0 0, 0

Sample Date 18

In summary, the results indicate that the chlorination system was effective in preventing growth and prolonged settlement of postveligers in the service water systems. Zebra mussel settlement on MSCW and Unit 1 ESW artificial substrates was slightly elevated for two brief periods when there was low sample flow. During the fall Unit 2 C17 Refueling Outage, service water system chlorination was secured due to diver cleaning of the circulating water intake forebay and valve maintenance on the Unit 2 Circulating Water Discharge Vault valves. Settlement was again brought under control when chlorination was resumed near the end of the outage. The results showed that even when the system was taken out of service for short periods of time for system maintenance, or that system TRC residuals fell below their target band of 0.08-0.6 ppm, settlement control was quickly re-established.

3.2.3 Biocide Treatment There were no biocide treatments in 2007.

3.2.4 Mechanical Cleaning During the Unit 2 C17 (September-November) refueling outage, divers were employed to mechanically clean sand, zebra mussels, and debris from the walls and floors of the Unit 2 Circulating Water Intake Forebays and Unit 2 Condenser Inlet Tunnels. The Unit 2 Condenser Inlet Tunnel was cleaned in its entirety. The Unit 2 Intake Forebay was cleaned on the east (plant) side of the traveling screens (Figure 3-3). This included areas of the Unit 2 Circulating Water Pump and Unit 2 ESW Pump bays. The west side (lake side) of traveling screen bays 1-7 and 2-1, and 2-2 and 2-3 were cleaned in their entirety to the trash racks. The area further west of the trash racks extending to the west wall of the 19

intake forebay was not cleaned as well as the west sides of traveling screen bays 2-4 and 2-5, and 2-6 and 2-7.

In the Fall of 2007, the divers cleaned the intake crib velocity caps, ice guards, and trash racks of zebra mussels to remove the food source that attracts wild ducks to the intake cribs.

20

Figure 3-3 Screenhouse Intake Forebay tK)

Note: Lined out areas were cleaned during the U2C17 Refueling Outage.

Chapter 4 Summary and Recommendations 4.1 Summary The 2007 Mollusc Biofouling Monitoring Program was initiated on 26 April and continued to 5 December. Heaviest spawning activity occurred during late-May to early June, mid-July, late August, and mid to late October. The major spawning peak occurred on 20 July.

Whole water veliger densities declined after their peak on 11 October With falling lake temperatures into December. The whole water densities show that there are substantial numbers of veligers in the forebay, indicating the need for effective chlorination in the service water systems. The 2003 report concluded that yearly results in peak abundances make it difficult to predict when the peak abundance will occur each season other than estimating some time between July and October. Continued whole-water monitoring during the veliger spawning season will detect when these peak abundances occur.

The intake forebay PVC sampler zebra mussel density was 129,425 ind./m 2. Individuals ranged from 193u-15,408u (. 19 mm - 15.41 mm) and the mean size of fifty randomly selected individuals was 3,328u (3.3 mm). As in 2006, the time period of collection was designed to coincide with the annual fall intake crib cleaning to estimate the size and density of mussels the divers might encounter at the time of cleaning. For comparison, the sample substrate that was pulled in 2006 had a density of 303,801 individuals/m 2 and an average size of 2,470u (2.5 mm). An explanation for this difference could be attributed to a large peak veliger density (455,000 ind./m 3 ) occurring in the Fall of 2005 that was more than twice the peak veliger density (191,800 ind./m 3 ) occurring in the Fall of 2006.

22

The data indicates that the chlorination system, when operational, was effective in preventing growth and prolonged settlement of postveligers in the service water systems.

The results showed that even when the system was taken out of service for short periods of time for system maintenance, or that system TRC residuals fell below their target band of 0.08-0.6 ppm, settlement control was quickly re-established.

Reports of visual inspections of heat exchangers performed during the Unit 2C1 7 Refueling Outage showed no live zebra mussel colonies residing in systems that were chlorinated.

4.2 Recommendations Based on observations made during the course of this program, the following recommendations are being made:

Whole-Water sampling should continue to be initiated in April to determine the presence of veligers in the water column, as currently implemented. The whole-water sampling frequency in 2005 was reduced from weekly to twice monthly in the months of June, October, and November to lessen the sampling burden and better target sampling based on previous years' spawning data. This sampling frequency reduction proved to be effective from 2005 through 2007 as the major spawning peaks were still able to be captured, but with less sampling and analysis effort. This reduced sampling schedule should be continued as currently implemented.

Studies of cumulative postveliger settlement should continue to be conducted from May through December, as currently implemented.

23

- Continuous chlorination maintained in the 0.08 -0.6 ppm target band should continue to run throughout the spawning season, as currently implemented.

Zebra mussel sampling and analysis in 2007 confirmed the efficacy of this target band. Chlorination system outages should be kept to a minimum to the extent possible to minimize the potential for trans-locator fouling in small bore piping systems.

- Maintain daily bio-box flow checks to ensure bio-box conditions are representative of system conditions.

- Chlorination data from all water systems (ESW, NESW, and MSCW) and temperature data should continue to be made available to allow meaningful interpretation of results.

24

References Lawler, Matusky, & Skelly Engineers LLP. 1995. Mollusc biofouling monitoring during 1994, Donald C. Cook Nuclear Plant: Final Report.

Great Lakes Environmental Center. 1996. A Zebra Mussel (Dreissena)

Monitoring Survey for the Donald C. Cook Plant April-December 1995: Final Report Lawler, Matusky,& Skelly Engineers LLP. 1997. Mollusc biofouling monitoring during 1996, Donald C. Cook Nuclear Plant: Final Report.

Lawler, Matusky, & Skelly Engineers LLP. 1999. Mollusc biofouling monitoring during 1998, Donald C. Cook Nuclear Plant: Final Report.

Grand Analysis. 1999. Zebra Mussel Monitoring Project for 1999.

Performed at Donald C. Cook Nuclear Plant. Final Report.

Grand Analysis. 2000. Mollusc Biofouling Monitoring Project for 2000.

Performed at Donald C. Cook Nuclear Plant. Final Report.

Grand Analysis. 2001. Mollusc Biofouling Monitoring Program for 2001.

Performed at Donald C. Cook Nuclear Plant. Final Report.

25

Grand Analysis. 2002. Mollusc Biofouling Monitoring Program for 2002.

Performed at Donald C. Cook Nuclear Plant. Final Report.

Grand Analysis. 2003. Mollusc Biofouling Monitoring Program for 2003.

Performed at Donald C. Cook Nuclear Plant. Final Report.

Cook Nuclear Plant Environmental. 2004. Mollusc Biofouling Monitoring Program for 2004. Performed at Donald C. Cook Nuclear Plant. Final Report.

Cook Nuclear Plant Environmental. 2005. Mollusc Biofouling Monitoring Program for 2005. Performed at Donald C. Cook Nuclear Plant. Final Report.

Cook Nuclear Plant Environmental. 2006. Mollusc Biofouling Monitoring Program for 2006. Performed at Donald C. Cook Nuclear Plant. Final Report.

26

Appendix Table 1 SWS ChInrin~tinn V~IIIA~ far 2007 ZAbr3 Mu~AI Monitorina Prnnr3m U-1 U-2 U-I ESW [C12] U-2 ESW [C12] NESW NESW East West East West [C12] [C12]

Date _l(ppm) (ppm) (ppm) (ppm) (ppm) (ppm) 5/2/2007 < 0.08 0.2 0.21 < 0.08 0.29 0.37 5/4/2007 0.13 0.12 0.11 0.08 0.33 0.36 5/7/2007 0.16 0.15 0.14 0.08 0.31 0.4 5/9/2007 0.09 0.21 0.22 0.1 0.22 0.24 5/11/2007 0.09 0.09 0.1 0.09 0.24 0.19 5/14/2007 0.15 0.12 0.12 0.13 < 0.08 0.19 5/16/2007 < 0.08 0.11 0.11 0.12 CI2 OFF 0.27 5/18/2007 < 0.08 0.32 0.32 < 0.08 5/21/2007 0.48 0.13 0.12 0.33 0.28 5/23/2007 0.13 0.14 0.12 0.14 0.09 0.24 5/25/2007 < 0.08 0.09 0.1 0.08 < 0.08 0.19 5/28/2007 0.12 0.1 0.1 0.08 < 0.08 0.14 5/30/2007 < 0.08 0.09 0.08 0.09 < 0.08 0.22 6/1/2007 0.1 0.1 0.11 0.17 < 0.08 0.19 6/4/2007 0.09 < 0.08 < 0.08 0.1 C12 ON 0.22 6/4/2007 0.13 6/6/2007 0.14 0.13 0.17 0.1 0.26 0.27 6/8/2007 0.16 0.12 0.09 0.24 0.2 0.27 6/11/2007 0.14 0.12 0.11 0.08 0.25 0.3 6/13/2007 0.12 0.16 0.12 0.14 0.24 0.24 6/15/2007 < 0.08 0.14 0.14 < 0.08 < 0.08 0.11 6/19/2007 0.13 0.13 0.11 0.09 0.21 0.23 6/20/2007 0.11 0.15 0.12 0.13 0.21 0.16 6/22/2007 < 0.08 0.13 0.12 0.15 0.12 0.25 6/25/2007 0.1 0.15 0.12 0.1 0.12 0.18 6/25/2007 C12 OFF C12 OFF C12 OFF C12 OFF C12 OFF C12 OFF 6/27/2007 C12 ON C12 ON C12 ON C12 ON C12 ON C12 ON 6/27/2007 0.09 0.09 0.1 < 0.08 0.17 0.23 6/29/2007 0.21 0.4 0.15 0.13 0.35 0.21 7/2/2007 0.14 0.14 0.29 0.3 0.29 0.34 7/4/2007 0.13 0.13 0.1 0.12 0.32 0.38 7/9/2007 < 0.08 0.14 27

Appendix Table 1 SWS Chlorination Values for 2007 Zebra Mussel Monitoring Program U-1 U-2 U-1 ESW [C12] U-2 ESW [C12] NESW NESW East West East West [C12] [Cl2]

Date _I(ppm) (ppm) m) (ppm) (ppm) (Ppm) 7/9/2007 0.13 < 0.08 0.25 0.33 7/11/2007 < 0.08 0.09 0.1 < 0.08 0.28 0.4 7/12/2007 < 0.08 0.08 7/13/2007 < 0.08 0.15 0.13 0.11 0.16 0.26 7/14/2007 0.09 0.18 0.16 0.19 7/16/2007 0.15 0.09 0.12 0.14 0.26 0.32 7/18/2007 0.17 0.14 0.12 0.14 0.34 0.37 7/20/2007 0.47 0.18 0.09 0.1 0.36 0.43 7/23/2007 0.13 0.17 0.13 0.09 0.41 0.44 7/25/2007 0.49 0.15 0.14 0.46 0.34 0.36 7/27/2007 0.15 0.14 0.12 < 0.08 0.26 0.3 7/30/2007 < 0.08 0.12 0.12 < 0.08 0.25 0.27 8/1/2007 < 0.08 0.08 0.09 < 0.08 0.16 0.08 8/3/2007 0.26 0.13 0.11 < 0.08 0.16 0.08 8/6/2007 0.22 0.13 < 0.08 0.13 0.25 0.29 8/8/2007 0.12 0.14 0.13 0.1 0.26 0.17 8/10/2007 < 0.08 0.15 0.22 < 0.08 0.25 0.21 8/13/2007 0.22 0.23 0.25 0.43 0.26 0.28 8/15/2007 0.13 0.14 0.22 < 0.08 0.22 0.3 8/17/2007 0.12 0.26 0.23 0.11 0.34 0.35 8/20/2007 0.12 0.48 0.56 0.09 0.35 0.49 8/22/2007 0.89 0.19 0.58 0.21 0.22 0.25 8/24/2007 0.1 0.25 0.22 0.1 0.21 0.32 8/27/2007 0.09 0.27 0.23 < 0.08 0.14 0.19 8/29/2007 0.13 0.25 0.16 0.13 0.09 < 0.08 8/31/2007 0.1 0.13 0.27 0.1 0.29 0.24 9/3/2007 0.34 0.16 0.16 0.09 0.14 0.1 9/5/2007 0.1 0.21 0.19 0.13 0.1 0.14 9/7/2007 < 0.08 < 0.08 < 0.08 < 0.08 < 0.08 < 0.08 9/10/2007 0.57 0.29 0.32 0.28 0.2 0.24 9/12/2007 < 0.08 0.29 0.32 < 0.08 0.22 0.26 9/13/2007 0.36 0.29 28

Appendix Table 1 SWS Chlorination Values for 2007 Zebra Mussel Monitoring Proram U-I U-2 U-I ESW [C12] U-2 ESW [C12] NESW NESW East West East West [C12] [CI2]

Date (ppm) (ppm) ppm m (ppm) (PPm) (ppm) 9/14/2007 C12 OFF C12 OFF C12 OFF C12 OFF C12 OFF C12 OFF CL2 CL2 CL2 CL2 CL2 CL2 10/1/2007 OFF OFF OFF OFF OFF OFF 10/31/2007 C12 ON C12 ON C12 ON C12 ON C12 ON C12 ON 11/1/2007 < 0.08 < 0.08 < 0.08 < 0.08 11/2/2007 0.31 0.14 0.13 0.29 0.15 0.21 11/5/2007 0.13 0.11 0.13 0.19 0.27 0.39 11/7/2007 0.49 0.08 0.1 0.25 0.24 0.35 11/9/2007 0.2 0.25 0.25 0.18 0.29 0.36 11/12/2007 0.18 0.16 0.15 0.16 0.2 0.24 11/14/2007 0.11 0.16 0.15 0.09 0.12 0.36 11/16/2007 0.48 0.34 0.13 0.28 0.15 0.41 11/19/2007 0.11 0.38 0.38 0.09 0.17 0.5 11/21/2007 0.08 0.32 0.26 0.08 0.16 0.45 11/23/2007 0.49 0.2 0.14 0.26 0.1 0.08 11/26/2007 0.13 0.15 0.17 0.09 < 0.08 < 0.08 11/28/2007 0.48 0.15 0.32 0.12 0.1 0.42 11/30/2007 0.17 0.28 0.26 0.16 0.29 0.36 29

APPENDIX IV NPDES APPLICATIONS 2007

STATE OF MICHIGAN DEPARTMENT OF ENVIRONMENTAL QUALITY LANSING JENNIFER M. GRANHOLM STEVEN E. CHESTER GOVERNOR DIRECTOR March 8, 2007 Mr. John Carlson Donald Cook Nuclear Plant 1 Cook Place Mail Zone 5A Bridgman, MI 49106

SUBJECT:

MODIFIED Groundwater Discharge Authorization for Donald Cook Nuclear Plant, Lake Township, Berrien County Enclosed is the modified Authorization to Discharge, GW1810102, issued by the Department of Environmental Quality (DEQ) on May 30, 2006 The following change has been made to the authorization:

Page 6 Process Wastewater has been added to Seepage Basins Location, and Sanitary Sewage Wastewater has been added to Rapid Infiltration Basins.

Please review carefully the conditions of the Authorization. In particular, please notice that any change in the discharge will require a new Authorization by the DEQ.

Questions concerning this Authorization can be directed to the Permits Section, Water Bureau (WB), telephone 517-373-8148, or the WB, Kalamazoo District Office, telephone: 269-567-3500.

Sincerely,

<-+

Ja es R. Janiczek, Chief Groundwater Permits Unit Water Bureau Enclosure cc: Mr. Blair Sordell, Cook Nuclear Lake Township Supervisor Mr. Gary Witkowski, Berrien County Health Department Mr. Greg Danneffel, DEQ - Kalamazoo

~orn

MICHIGAN DEPARTMENT OF ENVIRONMENTAL QUALITY WATER BUREAU GROUNDWATER DISCHARGE PERMIT This permit is issued under the provisions of Part 31, Water Resources -Protection, of the Natural Resources and Environmental Protection Act, 1994 PA 451, as amended (Act 451),

being Sections 324.3101 through 324.3119 of the Compiled Laws of Michigan, and the Administrative Rules promulgated thereunder. This permit does not relieve the permittee from obtaining and complying with any other permits required under local, state, or federal law.

Permit Number: GW1810102 Authorization Rule: 2218 Facility Name: Donald C. Cook Nuclear Plant Issue Date: May 30, 2006 Effective Date: June :1,2006 Expiration Date: June 1, 2009 Modified Date: March 5, 2007 Deadline for Submittal of Renewal Application: December 3, 2008 Facility Address: 1 Cook Place, Mail Zone 5A, Bridgman, Michigan 49106 Telephone: 269-465-5901, ext. 1153 Fax: 269-466-2550 Discharge Location

Description:

SW 1/4 of the SE 1/4 of Section 6, T06S, R19W, Lake Township, Berrien County, Michigan, as identified in Attachment 1 (Site Map) and fully described in this permit.

Permittee'Name: Indiana Michigan Power Permittee Address: One Summit Square, P.O. Box 60, Fort Wayne, IN 46801 Telephone: 260-421-1400 Fax: 260-425-2142 Authorization to discharge a maximum: 2.4 million gallons per day (876 million gallons per year) of process wastewater and 60,000 gallons per day (21.9 million gallons per year) of sanitary sewage in accordance with the limitations, monitoring requirements, and other conditions as set forth in this permit, Part 31, and its administrative rules.

Type of Wastewater #1: Process Type of Wastewater #2: Sanitary Sewage (Outfall 0OD) (Outfall OE)

Method of Treatment: Neutralization Method of Treatment: Sequencing Batch Reactor Method of Disposal: Seepage Basins Method of Disposal: Rapid Infiltration Basins In accordance with Section 324.3122 of the Michigan Act, the permittee shall make payment of an annual permit fee to the Department for each December 15 the permit is in effect regardless of occurrence of discharge. The permittee shall submit the fee in response to the Department's annual notice. The fee shall be postmarked by March 1 for notices mailed by January 15. The fee is due no later than 45 days after receiving the notice for notices mailed after January 15.

Fees paid in accordance with the Michigan Act are not refundable.

Donald C. Cook Nuclear Plan Page 2 of 13 All construction, maintenance, operations, and monitoring of this facility must comply with the conditions set forth in this permit or in plans approved by the Department in accordance with this permit. Failure to comply with the terms and provisions of this permit may result in civil and/or criminal penalties as provided in Part 31.

This permit is based upon the information submitted in the July 25, 2005, application for Groundwater Discharge received by the Michigan Department of Environmental Quality and any subsequent amendments. This permit supersedes Permit M 00988 issued to the facility on September 29, 2000.

Issued this 30t day of May 2006 ,for the Michigan Department of Environmental Quality.

Modified March 5, 2007 Groundwater Permits Unit Water Bureau Michigan Department of Environmental Quality

Donald C. Cook Nuclear Plan Page 3 of 13 A. Effluent Limitations and Monitoring Requirements The wastewater discharge shall be limited and monitored by the permittee, at a minimum, as specified below. The permittee shall submit reports monthly as specified in Section F. 1 of this permit. In the event of any non-compliance of limitations, including any detected in additional sampling to the minimum required below, the permittee shall fulfill the requirements of Section D.1 of this permit (Rule 2227)

SAMPLE LOCATION ID PARAMETER LIMITATION-UNITS I

Effluent Flows:

EQ-1 Flow 2,400,000 GPD Daily* Direct Measurement Process Wastewater (Turbine Room Sump) 876, 000, 000 GPY Annually Calculation (Outfall 0OD)

EQ-2 Flow 60,000 GPD Daily Direct Measurement Sanitary Sewage A Wastewater 21,900,000 GPY Annually Calculation

_OE)

(Outfall I Effluent Quality:

EQ-I Chloride*** mg/I Weekly Grab Process Wastewater (Turbine Room Sump)

(Outfall 0OD)

Ethanolamine mg/I Weekdays Grab Hydrazine ug/I Weekdays Grab pH*** 6.5-9.0 S.U.. Weekdays Grab Total Inorganic mg/I Monthly Calculation:

Nitrogen Ammonia (N) +

Nitrate (N) + Nitrite (N)

Ammonia Nitrogen mg/I Monthly Grab Nitrite Nitrogen mg/I Monthly Grab Nitrate Nitrogen mg/I Monthly Grab Sodium*** mg/I Twice/month** Grab Sulfate*** mg/I Twice/month** Grab

Donald C. Cook Nuclear Plan Page 4 of 13 SAMPLE PARAMETER LIMITATION- MEASUREMENT SAMPLE TYPE LOCATION ID UNITS FREQUENCY EQ-2 BOD5 35 mg/I Weekly Grab Sanitary Sewage Wastewater (Outfall 0OE)

Chloride*** mg/I Weekly Grab Dissolved Oxygen*** mg/I Weekly Grab Phosphorus 15 mg/I Weekly Grab PH*** 6.0-9.0 S.U. Weekly Grab Sodium*** mg/I Weekly Grab Total Inorganic Nitroger mg/I daily max Weekly Calculation:

Ammonia (N) +

Nitrate (N) + Nitrite (N)

Ammonia'Nitrogen mg/I Weekly Grab Nitrite Nitrogen mg/I Weekly Grab Nitrate Nitrogen mg/I Weekly Grab Land Application: Year Round LA-1 Seepage Basins Application Rate 26.0 gals/ft2 Daily Direct Measurement (Outfall OD)

LA-2 Rapid Infiltration Application Rate 7.5 gals/ft 2 Daily Direct Measurement (Basin A)

(Outfall ODE)

LA-3 Rapid Infiltration Application Rate 17.8 gals/ft 2 Daily Direct Measurement (Basin B)

(Outfall DDE) _

• The daily maximum is defined as the total discharge by weight, volume or concentration if specified, during any calendar day.

• • Sodium and sulfate shall be sampled once/month while the water treatment system regenerates and once/month when no regeneration occurs.

Refer to Section E., Item 1.

B. Groundwater Limitations and Monitoring Requirements The disposal of treated wastewater shall not cause the groundwater quality to exceed the limitations listed below. Groundwater monitoring wells EW-1A, EW-8, EW-12, EW-1 3, and EW-1 9 shall be sampled and the groundwater analyzed for the parameters listed below at least at the minimum frequencies indicated. Monitoring well EW-8 is the upgradient well until a new upgradient monitoring well is installed. Then both wells will be used. Compliance with limits established in this section will be measured at monitor wells EW-1A, EW-12, EW-13, and EW-19. Monitoring wells and groundwater flow direction are identified on Attachment 3 (Groundwater Monitoring Well Map). In the event of any non-compliance with limitations, including any detected in sampling additional to the minimum required below, the permittee shall fulfill the requirements of Section D. 1 of this permit (Rule 2227).

Donald C. Cook Nuclear Plan Page 5 of 13 PARAMETER LIMITATION MEASUREMENT SAMPLETYPE PARAMETER________UNIT - FREQUENCY SAMPLETYPE Static Water Elevation USGS - Ft Quarterly Direct Measurement pH 6.0-9.0 S.U. Quarterly Grab Chloride 250 mg/I Quarterly Grab Specific Conductance umhos/cm Quarterly Grab Total Inorganic Nitrogen 5 mg/I Quarterly Calculation:

Ammonia-N +

Nitrate-N + Nitrite-N Ammonia Nitrogen mg/I Quarterly Grab Nitrite Nitrogen 0.5 mg/I Quarterly Grab Nitrate Nitrogen mg/I Quarterly Grab Total Phosphorus 1 mg/I Quarterly Grab, Sulfate 250 mg/I Quarterly. Grab Dissolved Sodium 120 mg/I Quarterly Grab Total Dissolved Solids mg/I Quarterly Grab Total Alkalinity mg/I Annually Grab.

Bicarbonate mg/I Annually Grab Dissolved Calcium mg/I Annually Grab Dissolved Iron mg/I Annually Grab Dissolved Magnesium 200 mg/I Annually Grab Dissolved Oxygen mg/I Annually Grab Dissolved Potassium mg/I Annually Grab Total Organic.Carbon mg/I Annually Grab Phenols mg/I Annually Grab Ethanolamine 2 mg/I Annually Grab Dissolved Aluminum 150 ug/I Annually Grab Dissolved Barium 440 ug/I Annually Grab Dissolved Boron 1900 ug/I Annually Grab Dissolved Cadmium 2.2 ug/I Annually Grab Dissolved Chromium 11 ug/I Annually Grab Dissolved Copper 9 ug/I Annually Grab Dissolved Lead 10 ug/I Annually Grab Dissolved Manganese 530 ug/I Annually Grab Dissolved Inorganic 0.0013 ug/I Annually Grab Mercury Dissolved Nickel 52 ug/I Annually Grab Dissolved Selenium 5 ug/I Annually Grab Dissolved Silver 0.2 ug/I Annually Grab Dissolved Zinc 120 ug/I Annually Grab;,

Hydrazine 10 ug/I Annually Grab

Donald C. Cook Nuclear Plan Page 6 of 13 C. Observation Monitoring Requirements The permittee shall inspect the treatment and disposal facilities for the operational conditions required below at the minimum frequency specified. All inspections shall be documented in a logbook to be maintained at the on-site facility and shall be available for review by Department personnel at all times.

LOCATION CONDITION MEASUREMENT SAMPLE TYPE FREQUENCY Seepage Basins Oil Sheen Weekdays Visual Observation Process Wastewater (Outfall 0OD)

Dike Inspection Weekly Visual Observation Rapid Infiltration Vegetation Control Weekly Visual Observation Basins Sanitary Sewage Wastewater (Outfall OOE)

Dike Inspection Weekly Visual Observation Freeboard 2 feet minimum Direct Measurement D. Compliance Requirements If Permit Limits Are Exceeded

1. If a limit described in Section A or B is exceeded, the discharger shall comply with Rule 2227 and undertake the following within the specified timeframes indicated, below:

a. Provide written notification to the Department at the address in Section F.2 of this permit, within seven calendar days, that a limit has been exceeded. Such notification shall include the name of the substance(s), the concentration(s), and the location(s) that exceeded the limit(s).

b. Resample and analyze for the parameter(s) of concern, within 14 days, at the location where a limit was exceeded.

c. Submit a report to the Department at the address in Section F.2 of this permit, within 60 days. Such report shall include the results of confirmation sampling, an evaluation of the reasons for the limit being exceeded, and the steps taken or proposed to prevent recurrences.

d. Complete additional activities as may be required by the Department pursuant to Rule 2227(1)(d).

E. Schedule of Activities - The permittee shall undertake the following activities by the dates specified.

1. Within 90 days of permit issuance, the discharger shall be in compliance with permit effluent limits and monitoring requirements for chloride, dissolved oxygen, pH, sodium and sulfate.

-I

Donald C. Cook Nuclear Plan Page 7 of 13

2. Submit for review and receive approval for an updated Operation and Maintenance Manual by August 1, 2006 [Rule 2218(4)(b)].

3. Submit for review and receive approval of a work plan for the installation of monitoring wells by August 1, 2006.

4. Install monitoring wells as described in the approved work plan by September 1, 2006.

5. Submit for review and receive approval of a report of monitoring well installation by October 2, 2006.

6. Submit monitoring well sampling results for background water quality by December 1, 2006.

7. Submit for review and receive approval of Modifications to the Sampling and Analysis Plan by October l, 2006 (Rule 2223).

F. Submittal Requirements for Self Monitoring Data

1. The permittee shall submit self-monitoring data monthly on the Department's Compliance Monitoring Report (CMR) for each calendar month of the authorized discharge period to:

NMS-CMR-Data Entry-Groundwater and Kalamazoo District Water Bureau Water Bureau-MDEQ Michigan Department of Environmental Quality 7953 Adobe Road P.O. Box 30273 Kalamazoo, Michigan 49009-5026 Lansing, Michigan, 48909-7773 The forms shall be postmarked no later than the 15th day of the month following each month of the authorized discharge period(s).

Alternative Daily Discharge Monitoring Report formats may be used if they provide equivalent reporting details and are approved by the Department.

2. All other notices, plans, reports, and other submissions required by and pursuant to this permit shall be submitted to the following:

Kalamazoo District Office DEQ-Water Bureau 7953 Adobe Rd.

Kalamazoo, MI 49009-5026 Telephone: 269-567-3500 G. Other Conditions

1. Effluent shall be isolated from water supply wells as specified in Rule 2204(2)(d).

2. Effluent shall not be applied within 100 feet from property lines.

3. The permittee shall maintain all treatment or control facilities or systems installed or used by the discharger to achieve compliance with this permit in good working order and operate the facilities or systems as efficiently as possible.

Donald C. Cook Nuclear Plan 'Page 8 of 13

4. Pursuant to Rule 2223(1), the Department may modify the effluent or groundwater monitoring parameters or frequency requirements of this permit, or they may be modified upon the request of the permittee with adequate supporting documentation.

5. Prior to any land application of bulk bios0lids, the permittee shall submit to the Field Operation Section, Water Bureau, and receive approval of a Residuals Management Program (RMP) that complies with the requirements of the Part 24 Rules (R 323.2401 through R 323.2418 of the Michigan Administrative Code). The permittee is authorized to land apply bulk biosolids or prepare bulk biosolids for land application in accordance with an approved RMP.

H. Approved Documents - The following documents, previously submitted and approved are incorporated into this permit by reference. These documents, and those submitted and approved under Section E of this Permit, may be modified upon written approval of the Department.

1. Sampling and Analysis Plan - dated February 23, 2005.

2. Discharge Management Plan - dated July 18, 2005

1. Permit Application - Issuance of this permit is based upon the information submitted on the Application for Groundwater Discharge (Application) and any subsequent amendments received by the Department. Any material or intentional inaccuracies found in this information, or omissions of material information, may be grounds for the revocation or modification of this permit or other enforcement action.

The permittee shall inform the Department's Water Bureau, Kalamazoo District Supervisor, of any known material or intentional inaccuracies in the information of the Application which would affect the permittee's ability to comply with the applicable rules or license conditions. The following documents were submitted to the Department as part of the Application:

1. Basis of Design, Rule 2218 - dated November, 1994.

2. Hydrogeological Report, Rule 2221 - dated February 23, 2005.

3. Waste Characterization, Rule 2220 - 2004 Effluent Data.

4. Discharge Management Plan, Rule 2233 - dated July 18, 2005.

5. Restrictive Covenant - dated October 26, 2000 J. Transfer of Ownership -The permittee shall notify the Department, in writing, no less than 30 days before a change in ownership of the facility. This permit may be transferred to the new owner by written approval of the Chief of the Permits Section, Water Bureau.

K. Change or Modification of Treatment or Discharge - Rule 2218 (3)(d) and (e)

The permittee, if proposing to modify the quantity or effluent characteristics of the discharge, if proposing to modify the monitoring program, or if proposing to modify the treatment process for the discharge, shall notify the Department of the proposed modification before it occurs. The Department shall determine if the proposed modification requires the permit to be modified to ensure that the terms of Rule 2204 are met.

Donald C. Cook Nuclear Plan Page 9 of 13 Modifications determined by the Department to be significant require that the permittee submit an application for and obtain a reissuance of the permit before such modification occurs. For modifications determined by the Department to be minor based on the quantity or quality of the discharge, the permit may be modified by the Department as requested by the permittee without obtaining a reissuance of the permit before such modification occurs.

L. By-Passing Any diversion from or bypass of facilities necessary to maintain compliance with the terms and conditions of this permit is prohibited, except where unavoidable to prevent loss of life, personal injury, or severe property damage. The permittee shall immediately notify the Department of any such occurrence by telephone at 1-800-292-4706. Such notice shall be supplemented by a written report with the next operation report detailing the cause of such diversion or bypass and the corrective actions taken to minimize adverse impact and eliminate the need for future diversion or bypass.

M. Cessation of Discharge-Related Activities If all or any portion of the permitted treatment facilities and discharge areas is intended to be eliminated, the permittee shall comply with the requirements of Rule 2226.

NOTE:

IF THE PERMITTEE WISHES TO CONTINUE DISCHARGING BEYOND THE EXPIRATION DATE, THE PERMITTEE SHALL SUBMIT AN ADMINISTRATIVELY COMPLETE APPLICATION FOR REISSUANCE NO LATER THAN 180 DAYS PRIOR TO THE EXPIRATION DATE IN ACCORDANCE WITH RULE 2151 OF THE PART 21 ADMINISTRATIVE RULES. FAILURE TO SUBMIT AN ADMINISTRATIVELY COMPLETE APPLICATION FOR REISSUANCE BY THE REQUIRED DATE WILL RESULT IN TERMINATION OF THE AUTHORIZATION TO DISCHARGE ON THE EXPIRATION DATE.

k(0

Page 10 of 13 Donald C. Cook Nuclear Plant C ATTACHMENT 1 SITE MAP T7.

£.~

P'roperty, Boundaries Donald C. Cook Nuclear Plant.

Bridgman Michigan Lake Township Berrien Coumty I" = 0.5 mile

Donald C, Cook Nuclear Plant Page 11 of 13 ATTACHMENT 2 PROCESS FLOW DIAGRAM Makeup Plant.detail Lake Township Nonessential Service Water (Potable Water (NESVV)

(0.21 MGD)

Donald C. Cook Nuclear Plant rf- Page 12 of 13, ATTACHMENT 2 PROCESS FLOW DIAGRAM Sewage treatment plant detail Fire Protectiorn Lake Township Potable (Flushing and testing water is directed to Water Supply (0.21 MGD) storfnwater and/or groundwater)

Portable Toilet Wastes IMiscellaneous Rinsing operations

Donald C. CooK Nuclear F'ant - age 1,3 0 1a

(------. ATTACHMENT 3 GROUNDWATER MONITORING WELL MAP North W l12- --- *Well 19 Well 13.

?! ~~Abs. Pond Overflow "**i -

, , (  :--*. Sewage Lagoon B .

• i>

Well 1A

/C L,7-'.,

,t~.0 -.- "' 0* V 40,..

"-----Well 8

Indiana Michigan INDiANA Power Company INDIGANA INC111"N Cook Onle Nuclear Cook PlacePlant POWU'R Bridgman, MI 49106 Mr. Greg Danneffel, District Supervisor Michigan Department of Environmental Quality Surface Water Quality Division 7953 Adobe Road Kalamazoo, MI 49009 May 22, 2007 Re: American Electric Power Company Donald C. Cook Nuclear Plant NPDES Permit No. MI0005827

Dear Mr. Danneffel:

The following notification is being made pursuant to Cook Nuclear Plant's NPDES permit Part II.C.5 Compliance Dates Notification.

The U.S.EPA issued a letter on March 10, 2007, notifying the Regional Administrators and the delegated states the Section 316(b), Phase II rule was suspended and that such notification would be forthcoming in the Federal Register. Part I.A.1)0. of the Cook Nuclear Plant NPDES Permit requires the submittal of the information required by 40 CFR Part 122.21(r) (2), (3),. and (5) and 40 CFR Part 125.95 to the DEQ Permits Section. AEP is requesting that the Cook Nuclear Plant permit be modified by removing the entire text of Part I.A.10.

Please contact me at telephone (269) 465-5901 extension 2102 if you have any questions regarding this information.

Sincerely,-

Jon H. Harner Environmental Manager 2007-599

,Mr. Greg Danneffel 316b relief Page 2 May 22, 2007 I certify under penalty of law that I have personally examined and am familiar with the information submitted on this and all attached documents, and based on my inquiry of those individuals immediately responsible for obtaining the information, I believe the submitted information is true, accurate and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment.

H arner Environmental Manager 2007-599

Indiana Michigan NDIA4A Power Company Cook Nuclear Plant MYMORGANhOne Cook Place

" E Bridgman, MI 49106 Mr. Daniel Dell C/O Michigan Department of Environmental Quality Cashier's Office WB-NP2 P.O. Box 3.0657 Lansing, MI 48909-8157 September 25, 2007 Re: American Electric Power Company Donald C. Cook Nuclear Plant NPDES Permit No. MI0005827

Dear.Mr. Dell:

The following notification is being made pursuant to Cook Nuclear Plant's NPDES permit Part II.C.5 Compliance Dates Notification.

The U.S.EPA issued a letter on March 10, 2007, notifying the Regional Administrators and the delegated states the Section 316(b), Phase II rule was suspended and that such notification would be forthcoming in the Federal Register. Part I.A.10. of the Cook Nuclear Plant NPDES Permit requires the submittal of the information required by 40 CFR Part 122.21(r) (2), (3), and (5) and 40 CFR Part 125.95 to the DEQ Permits Section. AEP is requesting that the Cook Nuclear Plant permit be modified by changing the language in accordance with the suspension of the phase 2 rules.

We are enclosing the required application fee of $750. Please contact me at telephone (269) 465-5901 extension 2102 if you have any questions regarding this request.

Sincerely, Jon H. Harner Environmental Manager enclosure 2007-1159

Mr. Daniel Dell 316b relief Page 2 September 25, 2007 I certify under penalty of law that I have personally examined and am familiar with the information submitted on this and all attached documents, andbased on my inquiry of those individuals immediately responsible for obtaining the information, I believe the submitted information is true, accurate and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment.

J. H. Harner Environmental Manager 2007-1159

STATE OF. MICHIGAN DEPARTMENT OF ENVIRONMENTAL QUALITY

• LANSING JENNIFER M. GRANHOLM STEVEN E.'CHESTER GOVERNOR DIRECTOR October 4,. 2007 Mr. Jon Harner Donald C. Cook Nuclear Plant One Cook Place Bridgman, Michigan 49106

SUBJECT:

National Pollutant Discharge Elimination System (NPDES); Permit No. M10005827 Designated Name: American Elec Power-Cook PIt Enclosed is a copy of your draft NPDES permit and public notice. Please review these documents carefully. If you have any comments on the terms and conditions of this permit, including information that should be considered in the development of site-specific effluent limitations or comments on the standard permit language, please forward them to us prior to October 25, 2007, so that we~can consider them before the draft permit is placed on public notice.

The conditions of this draft permit have been considered on a case-specific basis, and the monitoring program is considered the minimum which will assure environmental protection.

Please note that we are now requesting that the permittee post the public notice of the permit at or nearby the entrance to the permitted facility. We will forward a copy of the public notice package when the permit is ready to be posted. This posting will complement our notice that will also appear on the Department of Environmental Quality (DEQ) Web site. Please let me know if this is not suitable, and we can discuss the other two alternatives, which are (1) Post the notice in the post office or other public building of the municipality nearest the premises of the applicant~in which the discharge is or will be located, or (2) Publish the notice in a local newspaper of general circulation.

If you have any questions, please contact me.

Sincerely, Alvin Lam Lakes Michigan and Superior Permits Unit Permits Section Water Bureau .. - - ,..

517-335-4132 al/sea

Enclosures:

Draft NPDES Permit Public Notice cc: Mr. John Vollmer, kalamazoo District Office, Water Bureau, DEQ (electronic)

File CONSTITUTION HALL - 525 WEST ALLEGAN STREET - P.O. BOX 30273 - LANSING, MICHIGAN 48909-7773 www.rnichigan.gov - (517) 241-1300

PUBLIC NOTICE Date:

Permit No. M10005827 American Elec Power-Cook Pit The Michigan Department of Environmental Quality proposes to modify a discharge permit issued to: the Indiana Michigan Power Company for the American Electric Power Company, Donald C. Cook Nuclear Plant, located at One Cook Place, Bridgman, Michigan 49106. The applicant generates and distributes electricity. The applicant discharges noncontact cooling water, process wastewater, miscellaneous low volume wastes, and storm.water runoff to Lake Michigan, in the NWl/4, SW1/4, Section 6, Town 6 S, Range 19 W, Berrien County.

The draft permit includes the following modifications to the previously issued permit: The requirements for cooling water intake structure have been revised to reflect the U.S. EPA suspension of the Section 316(b), Phase II rules and Part I.A.9., Deicing Discharge Authorization, Outfall 003, has been added in accordance with the minor permit modification on November 17, 2004.

Comments or objections to the draft permit received by will be considered in the final decision to issue the permit. Persons desiring information regarding the draft permit, procedures for commenting, or requesting a hearing, should contact:

Alvin Lam, Permits Section, Water Bureau, Department of Environmental Quality, P.O. Box 30273, Lansing, Michigan 48909, telephone: 517-335-4132, e-mail: lama@michigan.gov.

Copies of the permit application, public notice, and draft permit may be obtained via the Internet (http://www.michigan.gov/deqnpdes - click on 'Permits on Public Notice' which is under the Permits banner) or at the Water Bureau Kalamazoo District Office located at 7953 Adobe Road, Kalamazoo, Michigan 49009-5026, telephone: 269-567-3500.

PERMIT NO. M10005827 MICHIGAN DEPARTMENT OF ENVIRONMENTAL QUALITY AUTHORIZATION TO DISCHARGE UNDER THE NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM In compliance with the provisions of the Federal Water Pollution Control Act, as amended, (33 U.S.C. 1251 et seq; the "Federal Act"), Michigan Act 451, Public Acts of 1994, as amended (the."Michigan Act"), Parts 31 and 41, and Michigan Executive Orders 1991-31, 1995-4 and 1995-18, Indiana Michigan Power Company One Cook Place

'Bridgman, Michigan 49106 is authorized to discharge from the American Electric Power Company, Donald C. Cook Nuclear Plant, located at One Cook Place Bridgman, Michigan 49106 designated as American Elec Power-Cook Pit to the receiving water named Lake Michigan, in accordance with effluent limitations, monitoring requirements and other conditions set forth in this permit.

Unless specified otherwise, all contact with the Michigan Department of Environmental Quality (the "Department") required by this permit shall be made to the Kalamazoo District Supervisor of the Water Bureau. The Kalamazoo District Office is located at 7953 Adobe Road, Kalamazoo, Michigan 49009-5026, telephone: 269-567-3500, fax: 269-567-9440. Unless specified otherwise, all Department approvals specified in this permit shall be by the District Supervisor.

In accordance with Section 324.3120 of the Michigan Act, the permittee shall make payment of an annual permit fee to the Department for each October 1 the permit is in effect regardless of occurrence of discharge. The permittee shall submit the fee in response to the Department's annual notice. The fee shall be postmarked by January 15 for notices mailed by December 1. The fee is due no later than 45 days after receiving the notice for notices mailed after December 1. Fees paid in accordance with the Michigan Act are not refundable.

In accordance with Section 324.3118 of the Michigan Act, the permittee shall make payment of an annual storm water fee to the Department for each January 1 the permit is in effect regardless of occurrence of discharge. The permittee shall submit the fee in response to the Department's annual notice. The fee shall be postmarked by March 15 for notices mailed by February 1. The fee is due no later than 45 days after receiving the notice for notices mailed after February 1. Fees paid in accordance with the Michigan Act are not refundable.

Any person who is aggrieved by this permit may file a sworn petition with the Office of Administrative Hearings of the Michigan Department of Environmental Quality, setting forth the conditions of the permit which are being challenged and specifying the grounds for the challenge. The Department may reject any petition filed more than 60 days after issuance as being untimely.

This permit is based on a complete application submitted on April 1, 2003.

This modified permit takes effect immediately. The provisions of this permit are severable. After notice and opportunity for a hearing, this permit may be modified, suspended, or revoked in whole or in part during its term in accordance with applicable laws and rules. On its effective date this permit shall supersede NPDES Permit No. M10005827, expiring October 1, 2003, which is hereby revoked upon the effective date of this permit.

This permit and the authorization to discharge shall expire at midnight, October 1,2008. In order to receive authorization to dischar ,e~ond the date of expiration, the permittee shall submit an application which contains such information, forms, and fees as are required by the Department by April 4, 2008.

Issued September 24, 2004. Based on a request submitted on September 27, 2007, this permit was modified on I 0 -'2z-o o'!

William Creal, Chief Permits Section W ater-Brea&':-::-'"

PERMIT NO. MIO05827 Page Tof26ý...

PART I Section A. Effluent Limitations And Monitoring Requirements

1. Final Effluent Limitations, Monitoring Points 001A & 002A (Combined Discharge)

During the period beginning on the effective date of this permit and lasting until the expiration date of this permit, the permittee is authorized to discharge a maximum of one billion five hundred million (1,500,000,000) gallons per day of noncontact condenser cooling water, miscellaneous low volume waters, and storm water runoff from Monitoring Point 001A through Outfall 001; and a maximum of one billion eight hundred twenty million (1,820,000,000) gallons per day of noncontact condenser cooling water, miscellaneous lowv volume waters, and storm water runoff from Monitoring Point 002A through Outfall 002. Outfalls 001 and 002 discharge to Lake Michigan. Such discharge shall be limited and monitored by the permittee as specified below.

Maximum Limits for Maximum Limits for Quantity or Loading Quality or Concentration Frequency Sample Parameter Monthly Daily Units Monthly Daily Units of Analysis Type Flow (report) (report) MGD --- --- --- Daily Report Total Daily Flow Total Residual Oxidant (TRO)

During Chlorination - No Bromine Use Discharge Mode Continuous (greater than 160 min/day) --- .... 38 ug/1 5X/Week Grab Intermittent (less than/equal to 160 min/day) --- .... 200 ug/I 5X/Week Grab During Bromine Use - the discharge time of bromine shall not exceed 120 min/day Intermittent (less than/equal to 120 min/day) --- .... 50 ug/1 5X/Week Grab TRO Discharge Time --- (report) min/day 5X/Week Report Total Discharge Time Temperature Intake (report) OF Daily Reading Discharge --- (report) OF Daily Reading Heat Additionh 17,300 MBTU/hr Daily .Calculation Total Boroni (report) (report) ug/1 Weekly Per Grab Occurrence Outfall Observation (report) --- --- Daily Visual Minimum Maximum Daily Daily pH 6.5 9.0 S.U. Daily Grab

a. Narrative Standard The receiving water shall contain no unnatural turbidity, color, oil films, floating solids, foams, settleable solids, suspehd'ed solids, or deposits as a result of this discharge.

b. Monitoring Location Samples, measurements, and observations taken in compliance with the monitoring requirements above shall be taken prior to discharge to Lake Michigan.

PERMIT NO. M10005827 Page 3 of 26 PART I 0>-1 Section A. Effluent Limitations And Monitoring Requirements

c. Outfall Observation Any unusual characteristics of the discharge (i.e., unnatural turbidity, color, oil film, floating solids, foams, settleable solids, suspended solids, or deposits) shall be reported within 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> to the Department followed with a written report within five. (5) days detailing the findings of the investigation and the steps taken to correct the condition. For turbidity caused by the discharge of bentonite clay, the permittee is only required to verbally report to the Department.

d. Water Treatment Additives This permit does not authorize the discharge of water additives without approval from the Department. Approval of water additives is authorized under separate correspondence. Water additives include any material that is added to water used at the facility or to a wastewater generated by the facility to condition or treat the water. In the event a permittee proposes to discharge water additives, including an increased discharge concentration of a previously approved water additive, the permittee shallsubmit a request to the Department for approval. See Part L.A.6. for information on requesting water treatment additive use.

e. TRO (Chlorine and Bromirie) Requirements Total Residual Oxidant (TRO) shall be analyzed for using EPA Method 330.1 or Orion Electrode Model 97-70 (other analytical methods specified in 40 CFR 136 may be used upon approval of the Department). TRO monitoring is only required during periods of chlorine or bromine use and subsequent discharge. Limitations for the intermittent discharge of chlorine apply only when the discharge of chlorine is less than or equal to 160 minutes per day, otherwise the limitations for continuous discharge of chlorine apply. The intermittent discharge limitations shall apply when the service water system is chloriiated continuously and the noncontact condenser cooling water is chlorinated intermittently on the same day. Authorization to discharge bromine with or without chlorine is limited to 120 minutes per day at the limitations specified above with the additional requirement that any discharge of chlorine is restricted to a concurrent discharge with bromine (no additional discharge of chlorine is authorized for that day).

During the intermittent discharge of chlorine without bromine ("During Chlorination - No Bromine Use" limitations given above), the daily concentration value reported for TRO shall be the average of a minimum of three (3) equally spaced grab samples taken during a chlorine discharge event, with the additional limitation that no single sample may exceed 300 .tg/l.

During the intermittent discharge of bromine with or without chlorine ("During Bromine Use" limitations given above), the daily concentration value reported for TRO shall be the maximum of at least three (3) equally spaced grab samples taken during a bromine discharge event (no single sample may exceed 50 [ig/1).

The permittee shall enter a zero ("0") on the Discharge Monitoring Report for the TRO discharge modes not being used.

The permittee may use dehalogenation techniques to achieve the applicable TRO limitations, using sodium thiosulfate, sodium sulfite, sodium bisulfite, or other dehalogenating reagents approved by the Department. The quantity of reagent(s) used shall be limited to 1.5 times the stoichiometric amount of applied chlorine/bromine oxidant. Each month the permittee shall report the quantity of each dehalogenation reagent used per day.

f. Power Plants - PCB Prohibition Beginning upon the effective date of this permit, the permittee shall not discharge any polychlorinated biphenyls to the receiving waters of the state of Michigan as a result of plant operations other than 'due to the presence of such compounds in the intake water.

g.. Chemical Metal Cleaning Wastes The permittee shall not discharge chemical metal cleaning wastes to the receiving waters of the state of Michigan as a result of plant operations.

h. Heat Addition The daily maximum limit of 17,300 MBTU/hr is for the total power plant discharge. The permittee shall report the t eat'bad d arged through utflls 001 and 002 under Outfall 001, in addition to reporting the heat 16ad§!dischafgedlJnlivdually for the Outfalls 001 and-002, in the discharge monitoring reports.

PERMIT NO. M10005827 Page 4 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements

i. Total Boron Monitoring During ice melt operations, the permittee shall sample the monitoring tanks for Total Boron prior to discharge through Outfalls 001 and 002. The permittee shall quantify the number of gallons released and perform a mass balance calculation and report the calculated boron discharged through Outfalls 001 and 002 in ug/l.

Analytical Method and Quantification Level for Total Boron The sampling procedures, preservation and handling, and analytical protocol for compliance monitoring for Total Boron shall be in accordance with EPA Method 200.7. The quantification level for Total Boron shall be 20 ug/l unless a higher level is appropriate because of sample matrix interference. Justification for higher quantification levels shall be submitted to the Department within 30 days of such determination. Upon approval of the Department, the permittee may use alternate analytical methods (for parameters with methods specified in 40 CFR 136, the alternate methods are restricted to those listed in 40 CFR 136).

PERMIT NO. MI[0005827 Page 5 of 26ý-

PART I (0 -~-6-1 Section A. Effluent Limitations And Monitoring Requirements

2. Final Effluent Limitations, Monitoring Point OOA (Unit 1 Steam Generator Blowdown) and Monitoring Point OOB (Unit 2 Steam Generator Blowdown)

During the period beginning on the effective date of this permit and lasting until the expiration date of this permit, the permittee is authorized to discharge a maximum of one million (1,000,000) gallons per day of steam generator blowdown from Monitoring Point OOA through Outfalls 001 and 002; and one million (1,000,000) gallons per day of steam generator blowdown from Monitoring Point 00B through outfalls 001 and 002. Such discharge shall be limited and monitored by the permittee as specified below.

Maximum Limits for Maximum Limits for Quantity or Loading Quality or Concentration Frequency. Sample Parameter Monthly Daily Units Monthly Daily Units of Analysis Type Fiow (report) (report) MGD . Daily Report Total Daily Flow Outfall Observation (report) Daily Visual

a. Monitoring Location

• Samples, measurements, and observations taken in compliance with the monitoring requirements above shall be taken at Monitoring Points OOA and 00B prior to discharge to the intake forebay and thence to Outfalls 001 or 002.

PERMIT NO. M10005827 Piie,6 of 26 PART I La- K..

Section A. Effluent Limitations And Monitoring Requirements

3. Final-Effluent Limitations, Monitoring Point OOC (Plant Heating Boiler Blowdown)

During th*e period beginning on the effective date of this permit and lasting until the expiration date of this permit, the permittee is authorized to discharge a maximum of forty three thousand (43,000) gallons per day of heating boiler blowdown from Monitoring Point 00C through Outfalls 001 and 002. Such discharge shall be limited and monitored by the permittee as specified below.

Maximum Limits for Maximum Limits for Quantity or Loadin2 Quality or Concentration Frequency Sample Parameter Monthly Daily Units Monthly Daily Units of Analysis Type Flow (report) (report) MGD --- Daily Report Total Daily Flow Total Suspended Solidsb 30 100 mg/l Weekly Per Grab Occurrence

a. Monitoring Location Samples and measurements taken in compliance with the monitoring requirements above shall be taken at Monitoring Point OOC prior to discharge to the intake forebay and thence to Outfalls 001 or 002.

b. Total Suspended Solids Total Suspended Solids are to be monitored daily per occurrence or weekly if the heating boiler is operated continuously for periods greater than one week.

PERMIT NO. M10005827 Page 7 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements 1~

W4

4. Final Effluent Limitations, Monitoring Point OOG (Reverse Osmosis System Reject)

During the period beginning on the effective date of this permit and lasting until the expiration date of this permit, the permittee is authorized to discharge a maximum of three hundred sixty six thousand (366,000) gallons per day of reverse osmosis system reject from Monitoring Point 00G through Outfalls 001 and 002. Such discharge shall be limited and monitored by the permittee as specified below.

Maximum Limits for . Maximum Limits for Quantity Or Loading Quality or Concentration Frequency Sample Parameter Monthly Daily Units Monthly Daily Units of Analysis Type Flow (report) (report) MGD ..... "7 Daily Report Total Daily Flow Total Suspended Solids 30 100 mg/I Weekly Grab

a. Monitoring Location Samples and measurements taken in compliance with the monitoring requirements above shall be taken at Monitoring Point OOG prior to discharge to the intake forebay and thence to Outfalls 001 and 002.

PERMIT NO. M10005827 Page 8 of 26 PART I

[C)~ o Section A. Effluent Limitations And Monitoring Requirements

5. Final Effluent Limitations, Monitoring Point 00H (Turbine Room Sump Emergency Overflow)

During the period beginning on the effective date of this permit and lasting until the expiration date of this permit, the permittee is authorized to discharge a maximum of two millionsix hundred thousand (2,600,000) gallons per day of turbine room sump emergency overflow from Monitoring Point OOH through Outfalls 001 and 002. Such discharge shall be limited and monitored by the permittee as specified below.

Maximum Limits for Maximum Limits for Quantity or Loading Quality or Concentration Frequency Sample Parameter Monthly Daiiy Units Monthly Daily Units of Analysis Type Flow (report) (report) MGD Daily Per Report Total Occurrence Daily Flow (by Estimation)

Total Suspended Solids 30 100 mg/l 2X/Month Grab Per Occurrence Oil and Grease 15 20 mg/I 2X/Month Grab Per Occurrence 4., ,

a. Monitoring Location Samples and measurements taken in compliance with the monitoring requirements above shall be taken at Monitoring Point OOH prior to discharge to the intake forebay and thence to Outfalls 001 and 002.

b. Frequency of Analysis Samples and measurements shall be taken during discharge only.

PERMIT NO. M10005827 Page 9 of 26 PART I (0 -- Oo"]

Section A. Effluent Limitations And Monitoring Requirements

6. Request for Discharge of Water Treatment Additives In the event a permittee proposes to discharge water additives, .the permittee shall submit a request to discharge water additives to the Department for approval. Such requests shallbe sent to the Surface Water.Quality Assessment Section, Water Bureau, Department of Environmental Quality, P.O. Box 30273, Lansing, Michigan 48909, with a copy to the Department contact listed on the cover page of this permit. Instructions to submit a request electronically may be obtained via the Internet (http://www.michigan.gov/deq and on the left side of the screen click on Water, Water Quality Monitoring, and.Assessment of Michigan Waters; then click on the Water Treatment Additive List which is under the Information banner). Written approval from the Department to discharge such additives at specified levels shall be obtained prior to discharge by the permittee. Additional monitoring and reporting may be required as a condition for the approval to discharge the additive.

A request to discharge water additives shall include all of the following water additive usage and discharge information:

a. Material Safety Data Sheet;

b. the proposed water additive discharge concentration;

c. the discharge frequency (i.e., number of hours per day and number of days per year);

d. the monitoring point from which the product is to be discharged;

e. the type of removal treatment, if any, that the water additive receives prior to discharge;

f. product function (i.e. microbiocide, flocculant, etc.);

g. a 48-hour LC 5 0 or EC5 0 for aNorth American freshwater planktonic crustacean (either Ceriodaphniasp., Daphnia sp., or Sirnocephalussp.); and

h. the results of a toxicity test for one other North American freshwater aquatic species (other than a planktonic crustacean) that meets a minimum requirement of Rule 323.1057(2) of the Water Quality Standards.

Prior to submitting the request, the permittee may contact the Surface Water Quality Assessment Section by telephone at 517-3,35-1180 or via the Internet at the address given above to determine if the Department has the product toxicity data required by items g. and h. above. If the Department has the data, the permittee will not need to submit product toxicity data.

7. Temperature Modification The Department is considering the necessity of incorporating temperature limitations in this permit to assure that-the requirements of Rule 323.1082 of the Water Quality Standards are met. Therefore, when consideration of this issue has been completed, the Department may modify this permit in accordance with applicable lawsand rules to add appropriate temperature limitations or requirements.

8. Intake Screen Backwash, Outfall 004 During the period beginning on the effective date of this permit and lasting until the expiration date of this permit, the permittee is authorized to discharge intake screen backwash from outfall 004 to Lake Michigan. The permittee shall collect and remove debris accumulated on intake trash bars and dispose of such material on land in an appropriate manner.

9. Deicing Discharge Authorization, Outfall 003 The permittee is authorized to discharge a portion of the flow from outfall 001 or outfall 002 through intake structure outfall 003 to prevent ice buildup. The permittee is not required to provide any additional monitoring of this discharge because the effluent limitations and monitoring requirements specified for outfalls 001 and 002 will determine compliance with "applicable waterquality standards and any other requirements.

PERMIT NO. M10005827 Page 10 of 26

"- . PART 1 * - 7F290-Section A. Effluent Limitations And Monitoring Requirements

10. Storm Water Pollution Prevention Plan The permittee is authorized to discharge storm water associated with industrial activities as defined in 40 CFR 122.26(b)(14). These storm water discharges shall be controlled in accordance with the requirements of this special condition. The permittee has developed and implemented a Storm Water Pollution Prevention Plan (plan). The permittee shall continue implementation of the plan for maximum control of significant materials (as defined in Part I.A.10.i.) so that storm water discharges will not cause a violation of the Water Quality Standards. The plan shall be routinely reviewed and updated in accordance with the requirements of this Special Condition.

a. Source Identification To identify potential sources of significant materials that can enter storm water and subsequently be discharged from the facility, the plan shall, at a minimum, include the following:

1) A site map identifying the following: buildings and other permanent structures; storage or disposal areas for significant materials; secondary containment structures; storm water discharge outfalls (numbered for reference); location of storm water inlets contributing to each outfall; location of NPDES permitted discharges other than storm water; outlines of the drainage areas contributing to each outfall; structural runoff controls or storm water treatment facilities; areas of vegetation; areas of exposed and/or erodible soils; impervious surfaces (roofs, asphalt, concrete); name and location of receiving water(s); and areas of known or suspected impacts on surface waters as designated under Part 201 (Environmental Response) of the Michigan Act.

2) A list of all significant materials that could enter storm water. For each material listed, the plan shall include the following descriptions:

a) ways in which each type of material has been or has reasonable potential to become exposed to storm water (e.g., spillage during handling; leaks from pipes, pumps" and vessels; contact with storage piles; waste handling and disposal; deposits from dust or overspray, etc.);

b*) identification of the outfall or outfalls through which the material may be discharged if released; c) a listing of spills and leaks of polluting materials in quantities reportable under the Part 5 Rules (Rules 324.2001 through 324.2009 of the Michigan Administrative Code) that occurred at areas that are exposed to precipitation or that otherwise discharge to a point source at the facility. The listing shall include spills and leaks that occurred over the three (3) years prior to the completion of the plan or latest update of the plan; the date, volume and exact location of release; and the action taken to clean up the material and/or prevent exposure to storm water runoff or contamination of surface waters of the state. Any release that occurs after the plan has been developed shall be controlled in accordance with the plan and is cause for the plan to be updated as appropriate within 14 calendar days of obtaining knowledge of the spill or loss; and d) a summary of existing storm water discharge sampling data (if available) describing pollutants in storm water discharges associated with industrial activity at the facility. This summary shall be accompanied by a description of the suspected source(s) of the pollutants detected.

3) An evaluation of the reasonable potential for contribution of significant materials to runoff from at least the following areas or activities: loading, unloading, and other material handling operations; outdoor storage, including secondary containment structures; outdoor manufacturing or processing activitie.s; significant dust or particulate generating processes; discharge from vents, stacks and air emission controls; on-site waste disposal practices; maintenance and cleaning of vehicles, machines and equipment; sites of exposed and/or erodible soil;'

sites of environmental contamination listed under Part 201 (Environmental Response) of the Michigan Act; areas of significant material residue; and other areas where storm water may contact significant materials.

PERMIT NO. M10005827 Page I Iof 26 PART I Section A. Effluent Limitations And Monitoring Requirements

b. Preventive Measures and Source Controls, Non-Structural To prevent significant materials from contacting storm water at the source, the plan shall, at a minimum, include the following non-structural controls:

1) Description of a program for routine preventive maintenance which includes requirements for inspection and maintenance of storm water management and control devices .(e.g., cleaning of oil/water separators and catch basins) as well as inspecting and testing plant equipment and systems to uncover conditions that could cause breakdowns or failures resulting in discharges of pollutants to surface waters. A log of the inspection and corrective actions shall be maintained on file by the permittee, and shall be retained in accordance with Part I.A. 10.f.

2) A schedule for comprehensive site inspection to include visual inspection of equipment, plant areas, and structural pollution prevention and treatment controls to be performed at least once every six (6) months. A report of the results of the comprehensive site inspection shall be prepared and retained in accordance with Part I.A. I0.f.

The report shall identify any incidents of non-compliance with the plan. If there are no reportable incidents of non-compliance, the report shall contain a certification that the facility isin compliance with this plan.

3) A description of good housekeeping proceduresto maintain a clean, orderly facility.

4) A description of material handling procedures and storage requirements for significant materials.

Equipment and procedures for cleaning up spills shall be identified in the plan and made available to the appropriate personnel. The procedures shall identify measures to preventthe spilled materials or material residues on the outside of containers from being discharged into storm water. The plan may include, by reference, requirements of either a Pollution Incident Prevention Plan (PIPP) prepared in accordance with the Part 5 Rules (Rules 324.2001 through 324.2009 of the Michigan Administrative Code); a Hazardous Waste Contingency Plan prepared in accordance with 40 CFR 264 and 265 Subpart D, as required by Part I Il of the Michigan Act; or a Spill Prevention Control and Countermeasure (SPCC) plan prepared in accordance with 40 CFR 112.

5) Identification of areas that, due to topography, activities, or other factors, have a high potential for significant soil erosion. The plan shall also identify measures used to control soil erosion and sedimentation.

6) A description of employee training programs which will be implemented to inform appropriate personnel at all levels of responsibility of the components and goals of the plan. The plan shall identify periodic dates for such training.

7) Identification of significant materials expected to be present in storm water discharges following implementation of non-structural preventative measures and source controls.

c. Structural Controls for Prevention and Treatment Where implementation of the measures required by Part I.A. IO.b. does not control storm water discharges in accordance with Water Quality Standards in Part I.A. 10.h., the plan shall provide a description of the location, function, and design criteria of structural controls for prevention and treatment. Structural controls may be necessary:

1)- to prevent uncontaminated storm water from contacting or being contacted by significant materials, and/or

2) if preventive measures are not feasible or are inadequate to keep significant materials at the site from contaminating storm water. Structural controls shall be used to treat, divert, isolate, recycle, reuse or otherwise manage storm water in a manner that reduces the level of significant materials in the storm water and provides compliance with the Water Quality Standards in accordance with Part I.A.10.h.

d. Keeping Plans Current

1) The permittee shall review the plan on or before November 4 th of each year, and maintain written summaries of the reviews. Based on the review, the permittee shall amend the plan as needed to ensure continued compliance with the terms and conditions of this permit.

PERMIT NO. M10005827 Page 12 of 26 PART I (0 Section A. Effluent Limitations And Monitoring Requirements

2) The plan shall also be updated or amended whenever changes or spills at the facility increase or have the potential to increase the exposure of significant materials to storm water, or when the plan is determined by the permittee or the Department to be ineffective in achieving the general objectives of controlling pollutants in storm water discharges associated with industrial activity. Updates based on increased activity at the facility shall include a description of how the permittee intends to control any new sources of significant materials or respond to and prevent spills in accordance with the requirements of Parts I.A.10;a., I.A. I0.b,, and I.A. 10.c.

3) The Department'or authorized representative may notify the permittee at any time that the plan does not meet minimum requirements. Such notification shall identify why the plan does not meet minimum requirements..

The permittee shall make the required changes to the plan within 30 days after such notification from the Department or authorized representative, and shall submit to the Department a written certification that the requested changes have been made.

e. Certified Storm Water Operator Update If the certified operator has changed or an additional certified stornm water operator is added, the permit-tee shall provide the name and certification number of the new operator to the Department. The new operator shall review and sign the plan.

f. Signature and Plan Review

1) The plan shall be signed by the certified storm water operator and by either the permittee or an authorized representative in accordance with 40 CFR 122.22. The plan shall be retained on site of the facility that generates the storm water discharge.

2) The permittee shall make plans, reports, log books, runoff quality data, and supporting documents available upon request to the Department or authorized representative.

g. Record Keeping The permittee shall maintain records of all inspection and maintenance activities. Records shall also be kept describing incidents such as spills or other discharges that can affect the quality of storm water runoff. All such records shall be retained for three (3) years.

h. Water Quality Standards At the time of discharge, there shall be no violation of the Water Quality Standards in the receiving waters as a result of this discharge. This requirement includes, but is not limited to, the following conditions:

1) In accordance with Rule 323.1050 of the Water Quality Standards, the receiving waters shall not have any of the following unnatdral physical properties in quantities which are or may become injurious-to any designated use: unnatural turbidity, color, oil film, floating solids, foams, settleable solids, suspended solids, or deposits as a result of this discharge.

2) Any unusual characteristics of the discharge (i.e., unnatural turbidity, color, oil film, floating solids, foams, settleable solids, suspended solids, or deposits) shall be reported within 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> to the Department followed with a written report within five (5) days detailing the findings of the investigation and the steps taken to correct the condition.

Significant Materials Significant Materials means any material which could degrade or impair water quality, including but not limited to:

raw materials; fuels; solvents, detergents, and plastic pellets; finished materials such as metallic products; hazardous substances designated under Section 101 (14) of Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) (see 40 CFR 372.65); any chemical the facility is required to report pursuant' to Section 313 of Emergency Planning and Community Right-to-Know Act (EPCRA); polluting materials as identified under the Part 5 Rules (Rules 324.2001 through 324.2009 of the Michigan Administrative Code);

Hazardous Wastes as defined in Part IIl of the Michigan Act; fertilizers; pesticides; and waste products such as ashes, slag, and sludge that have the potential to be released with storm water discharges.

PERMIT NO. M10005827 Page 13 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements

j. Prohibition of Non-storm Water Discharges Discharges of material other than storm water shall be in compliance with an NPDES permit issued for the discharge. Storm water shall be defined to include the following non-storm water discharges provided pollution prevention controls for the non-storm water component are identified in the plan: discharges from fire hydrant flushing, potable water sources including water line flushing, fire system test water, irrigation drainage, lawn watering, routine building wash down which does not use detergents or other compounds, pavement wash water where spills or leaks of toxic or hazardous materials have not occurred (unless all spilled material have been removed) and where detergents are not used, air conditioning condensate, springs, uncontaminated groundwater, and foundation or footing drains where flows are not contaminated with process materials such as solvents.

-Discharges from fire fighting activities are authorized by this permit, but do not have to be identified in the plan.

11. Cooling Water Intake Structure This condition establishes the program necessary for proceeding toward compliance with the regulations under Section 316(b) of the Clean Water Act. The purpose of these regulations is to establish the best technology available for minimizing adverse environmental impact associated with the use of cooling water intake structures.

The permittee submitted the Proposal for Information Collection on June 13, 2005. The permittee shall submit the information regarding Source Water Physical Data, Cooling Water Intake Structure Data, Cooling Water System Data, and Impingement Mortality and/or Entrainment Characterization Study report on or before April 4, 2008. The permittee shall also submit the remaining applicable portions of the Comprehensive Demonstration Study upon notification by the Department. When notifying the permittee, the Department will allow the permittee reasonable amount of time in order to complete the necessary tasks.

Based on the review of the above information, the Department will determine appropriate requirements and conditions, such as specified below, to be included in the permit, either by modification or reissuance of the permit.

a. Cooling water intake structure requirements,

b. Monitoring conditions,

c. Record keeping and reporting, and

d. Biennial status report.

PERMIT NO. M10005827 Page 14 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements Facility Contact The "Facility Contact" was specified in the application. The permittee may replace the facility contact at any time, and shall notify the Department in writing within 10 days after replacement (including the name, address and telephone number of the new facility contact).

a. The facility contact shall be (or a duly authorized representative of this person):

• for a corporation, a principal executive officer of at least the level of vice president, or a designated representative, if the representative is responsible for the overall operation of the facility from which the

- discharge described in the permit application or other NPDES form originates, o for a partnership, a general partner, 0 for a sole proprietorship, the proprietor, or o for a municipal, state, or other public facility, either a principal executive officer, the mayor, village president, city or village manager or other duly authorized employee.

b. A person is a duly authorized representative only if:

o the authorization is made in writing to the Department by a person described in paragraph a. of this section; and

• the authorization specifies either an individual or a position having responsibility for the overall operation of the regulated facility or activity such as the position of plant manager, operator of a well or a well field, superintendent, position of equivalent responsibility, or an individual or position having overall responsibility for environmental matters for the facility (a duly authorized representative may thus be either a named individual or any individual occupying a named position).

Nothing in this section obviates the permittee from properly submitting reports and forms as required by law.

The Michigan Department of Environmental Quality (DEQ) encourages you to consider submitting your monthly Discharge Monitoring Reports (DMRs) electronically using the new web based Electronic Environmental Discharge Monitoring Reporting (e2-DMR) System. Explore the many benefits of this faster, more efficient alternative to submitting hand-written or paper-based Discharge Monitoring Reports. Participating in e2-Reporting will:

RI Improve Data Quality 2 Save Time 2 Reduce Compliance Costs 2 Better Utilize State Resources 2 Allow for Immediate Feedback 2 Improve Compliance Assessment As a fully operational electronic reporting system, all of the necessary legal, security, and electronic signature functionalities have been included for this system to serve as a completely paperless reporting system. A complete Facility ParticipationPackagewhich includes information regarding minimum system requirements and registration information can be found on the e2-DMR System Website. To access this document simply type in the following URL on the address line of your internet browser: https://secure 1 state.mi.us/e2rs/

Then click on "about e-DMR" to download.the Facility ParticipationPackage.

PERMIT NO. M10005827 Page 15 of 26 PART II to Section A. Definitions This list of definitions may include terms not applicable to this permit.

Acute toxic unit (TUa) means 100/LC5 0 where the LC 50 is determined from a whole effluent toxicity (WET) test which produces a result that is statistically or graphically estimated to be lethal to 50% of the test organisms.

Bioaccumulative chemical of concern (BCC) means a chemical which, upon entering the surface waters, by itself or as its toxic transformation product, accumulates in aquatic organisms by a human health bioaccumulation factor of more than 1000 after considering metabolism and other physiochemical properties that might enhance or inhibit bioaccumulation. The human health bioaccumulation factor shall be derived accordingto R 323.1057(5). Chemicals with half-lives of less than 8 weeks in the water column, sediment, and biota are not BCCs. The minimum bioaccumulation concentration factor (BAF) information needed to define an organic chemical as a BCC is either a field-measured BAF or aBAF derived using the biota-sediment accumulation factor (BSAF) methodology. The minimum BAF information needed to define an inorganic chemical as a BCC, including an organometal, is either a field-measured BAF or a laboratory-measured bioconcentration factor (BCF). The BCCs to which these rules apply are identified in Table 5 of R 323.1057.of the Water Quality Standards.

Biosolids are the solid, semisolid, or liquid residues generated during the treatment of sanitary sewage or domestic sewage in a treatment works. This includes, but is not limited to, scum or solids removed in primary, secondary, or advanced wastewater treatment processes and a derivative of the removed scum or solids.

Bulk biosolids means biosolids that are not sold or given away in a bag or other container for application to a lawn or home garden.

Chronic toxic unit (TU,) means 100/MATC or 100/IC 25, where the maximum acceptable toxicant concentration (MATC) and IC25 are expressed as a percent effluent in the test medium.

Class B Biosolids refers to material that has met the Class B pathogen reduction requirements or equivalent treatment by a Process to Significantly Reduce Pathogens (PSRP) in accordance with the Part 24 Rules. Processes include aerobic digestion, composting, anaerobic digestion, lime'stabilization and air drying.

Daily concentration is the sum of the concentrations of the individual samples of a parameter divided by the number of samples taken during any calendar day. If the parameter concentration in any sample is less than the quantification limit, regard that value as zero when calculating the daily concentration. The daily concentration, will be used to determine compliance with any maximum and minimum daily concentration limitations (except for pH and dissolved oxygen). When required by the permit, report the maximum calculated daily concentration for'the month in the "MAXIMUM" column under "QUALITY OR CONCENTRATION" on the Discharge Monitoring Reports (DMRs).

For pH, report the maximum value of any individual sample taken during the month in the "MAXIMUM" column under "QUALITY OR CONCENTRATION" on the DMRs and the minimum value of any individual sample taken during the month in the "MINIMUM" column under "QUALITY OR CONCENTRATION" on the DMRs. For dissolved oxygen, report the minimum concentration of any individual sample in the "MINIMUM" column under "QUALITY OR CONCENTRATION" on the DMRs...

Daily loading is the total discharge by weight of a parameter discharged during any calendar day. This value is calculated by multiplying the daily concentration by the total daily flow and by the appropriate conversion factor.. The daily loading will be used to determine compliance with any maximum daily loading limitations. When required by the permit, report the maximum calculated daily loading for the month in the "MAXIMUM" column under "QUANTITY OR LOADING" on the DMRs.

Department means the MichiganDepartment of Environmental Quality.

Detection Level means the lowest concentration or amount of the target analyte that can be determined to be different from zero by a single measurement at a stated level of probability.

EC 5 0 means a statistically or graphically estimated concentration that is expected to cause I or more specified effects in 50% of a group of organisms under specified conditions.

PERMIT NO. M10005827 Page 16 of 26 PART II Section A. Definitions -

Fecal coliform bacteria monthly is the geometric mean of the samples collected in a calendar month (or 30 consecutive days). The calculated monthly value will be used to determine compliance with the maximum monthly fecal coliform bacteria limitations. When required by the permit, report the calculated monthly value in the "AVERAGE" column under "QUALITY OR CONCENTRATION" on the DMRs.

Fecal coliform bacteria 7-day is the geometric mean of the samples collected in any 7-day period. The calculated 7-day value will be used to determine compliance with the maximum 7-day fecal coliform bacteria limitations. When required by the permit, report the maximum calculated 7-day concentration for the month in the "MAXIMUM" column under "QUALITY OR CONCENTRATION" on the DMRs.

Flow Proportioned sample is a composite sample with the sample volume proportional to the effluent flow.

Grab sample is a single sample taken at neither a set time nor flow.

IC 25 means the toxicant concentration that would cause a 25% reduction inma nonquantal biological measurement for the test population.

Interference is a discharge which, alone or in conjunction with a discharge or discharges from other sources, both:

1) inhibits or disrupts the POTW, its treatment processes or operations, or its sludge processes, use or disposal; and

2) therefore, is a cause of a violation of any requirement of the POTW's NPDES permit (including an increase in the magnitude or duration of a violation) or, of the prevention of sewage sludge use or disposal.in compliance with the following statutory provisions and regulations or permits issued thereunder (or more stringent state or local regulations):

Section 405 of the Clean Water Act, the Solid Waste Disposal Act (SWDA) (including Title II, more commonly referred to as the Resource Conservation and Recovery Act (RCRA), and. including state regulations contained in any state sludge management plan prepared pursuant to Subtitle D of the SWDA), the Clean Air Act, the Toxic Substances Control Act, and the Marine Protection, Research and Sanctuaries Act. [This definition does not apply to sample matrix interference.]

Land Application means spraying or spreading biosolids or a biosolids derivative onto the land surface, injecting below the land surface, or incorporating into the soil so that the biosolids or biosolids derivative can either condition the soil or fertilize crops or vegetation grown in the soil.

LC 50 means a statistically or graphically estimated concentration that is expected to be lethal to 50% of a group of organisms under specified conditions.

Maximum acceptable toxicant concentration (MATC) means the concentration obtained' by calculating the geometric mean of the lower and upper chronic limits from a chronic test. A lower chronic limit is the highest tested concentration that did not cause the occurrence of a specific adverse effect;- An upper chronic limit is the lowest tested concentration which did cause the occurrence of a specific adverse effect and above which all tested concentrations caused such an occurrence.

MGD means million gallons per day.

Monthly frequency of analysis refers to a calendar month. When required by this permit, an analytical result, reading, value or observation must be reported for that period if a discharge occurs during that period.

Monthly concentration is the sum of the daily concentrations determined during a reporting month (or 30 consecutive days) divided by the number of daily concentrations determined. The calculated monthly concentration will be used to determine compliance with any maximum monthly concentration limitations. When required by the permit, report the calculated monthly concentration in the "AVERAGE" column under "QUALITY OR CONCENTRATION" on the DMRs.

For minimum percent removal requirements, the monthly influent concentration and the monthly effluent concentration shall be determined. The calculated monthly percent removal, which is equal to 100 times the quantity [I minus the quantity (monthly effluent concentration divided by the monthly influent concentration)], shall be reported in the "MINIMUM" column( under "QUALITY OR CONCENTRATION" on the DMRs.

PERMIT NO. M10005827 Page 17 of 26 PART II Section A. Definitions Monthly loading is the sum of the daily loadings of a parameter divided by the number of daily loadings determined in the reporting month (or 30 consecutive days). The calculated monthly loading will be used to determine compliance with any maximum monthly loading limitations. When required by the permit, report the calculated monthly loading in the "AVERAGE" column under "QUANTITY OR LOADING" on the DMRs.

National Pretreatment Standards are the regulations promulgated by or to be promulgated by the Federal Environmental Protection Agency pursuant to Section 307(b) and (c) of the Federal Act. The standards establish nationwide limits for specific industrial categories for discharge to a POTW.

NOAEL means the highest tested dose or concentration of a substance that results in no observed adverse effect in exposed test organisms where higher doses or concentrations result in an adverse effect.

Noncontact Cooling Water is water used for cooling which does not come into direct contact with any raw material, intermediate product, by-pSroduct, waste product or finished product.

Nondomestic user is any discharger to a POTW that dischargeswastes other than or in addition to water-carried wastes from toilet, kitchen, laundry, bathing or other facilities used for household purposes.

Pretreatment is reducing the amount of pollutants, eliminating pollutants, or altering the nature of pollutant properties to a less harmful state prior to discharge into a public sewer. The reduction or alteration can be by physical, chemical, or biological processes, process changes, or by other means. Dilution is not considered pretreatment unless expressly authorized by an applicable National Pretreatment Standard for a particular industrial category.

POTW is a publicly owned treatment works.

Quantification level means the measurement of the concentration of a contaminant obtained by using a specified laboratory procedure calculated at a specified concentration above the detection level. It is considered the lowest concentration at which a particular contaminant can be quantitatively measured using a specified laboratory procedure for monitoring of the contaminant.

Quarterly frequency of analysis refers to a three month period, defined as January through March, April through June, July through September, and October through December. When required by this permit, an analytical result, reading, value or observation must be reported for that period if a discharge occurs during that period.

Regional Administrator is the Region 5 Administrator, U.S. EPA, located at R-19J, 77 W. Jackson Blvd., Chicago, Illinois 60604.

Significant industrial user is a nondomestic user that: 1) is subject to Categorical Pretreatment Standards under 40 CFR

.403.6 and 40 CFR Chapter I, Subchapter N; or 2) discharges an average of 25,000 gallons per day or more of process wastewater to a POTW (excluding sanitary, noncontact cooling and boiler blowdown wastewater); contributes a process wastestream which makes up five (5) percent or more of the average dry weather hydraulic or organic capacity of the POTW treatment plant; or is designated as such by the permittee as defined in 40 CFR 403.12(a) on the basis that the industrial user has a reasonable potential for adversely affecting the POTW's treatment plant operation or violating any pretreatment standard or requirement (in accordance with 40 CFR 403.8(f)(6)).

Tier I value means a value for aquatic life, human health or wildlife calculated under R 323.1057 of the Water Quality Standards using a tier I toxicity database.

Tier II value means a value for aquatic life, human health or wildlife calculated under R 323.1057 of the Water Quality.

Standards using a tier II toxicity database.

Toxicity Reduction Evaluation, (TRE) means a site-specific study conducted in a stepwis6 process designed to identify the causative agents of effluent toxicity, isolate the sources of toxicity, evaluate the effectiveness of toxicity control options, and then confirm the reduction in effluent toxicity.

-PERMIT NO. M10005827 Page 18 of 26 PART IIV.-Iiff T Section A. Definitions Water Quality Standards means the Part 4 Water Quality Standards promulgated pursuant to Part 31 of Act No. 451 of the Public Acts of 1994, as amended, being Rules 323.1041 through 323.1117 of the Michigan Administrative Code.

Weekly frequency of analysis refers to a calendar week which begins on Sunday and ends on Saturday. When required by this permit, an analytical result, reading, value or observation must be reported for that period if a discharge occurs during that period.

Yearly frequency of analysis refers to a calendar year beginning on January I and ending on December 31. When required by this permit, an analytical result, reading, value or observation must be reported for that period if a discharge occurs during that period.

24-Hour Composite sample is a flow proportioned composite sample consisting of hourly or more frequent portions that ate taken over a 24-hour period.

3-Portion Composite sample is a sample consisting of three equal volume grab samples collected at equal intervals over an 8-hour period.

7-day concentration is the sum of the daily concentrations determined during any 7 consecutive days in a reporting month divided by the number of daily concentrations determined. The calculated 7-day concentration will be used to determine compliance with any maximum 7-day concentration limitations. When required by the permit, report the maximum calculated 7-day concentration for the month in the "MAXIMUM" column under "QUALITY OR CONCENTRATION" on the DMRs.

7-day loading is the sum of the daily loadings of a parameter divided by the number of daily loadings determined during any 7 consecutive days in a reporting month. The calculated 7-day loading will be used to determine compliance with any maximum 7-day loading limitations. When required by the permit, report the maximum calculated 7-day loading for the month in the "MAXIMUM" column under "QUANTITY OR LOADING" on the DMRs.

Preventing Pollution is the Best Solution The Michigan Department of Environmental Quality (DEQ) encourages you to consider pollution prevention alternatives. In some cases pollution prevention may allow you to avoid the need to discharge pollutants which would otherwise require permit limitations -- or even avoid the need for permits altogether! Pollution prevention can:

El Save Money El Reduce Waste El Aid Permit Compliance 1l Protect Our Environment Zl Improve Corpora-te Image El Reduce Liability The DEQ is helping Michigan's industries save money, reduce waste and protect our environment through pollution prevention. DEQ staff can provide pollution prevention assistance through telephone consultations, technical workshops and seminars, and informational publications. They can also put you directly in touch with local support networks and national pollution prevention resources. For more information, contact the Michigan Department of Environmental Quality, Environmental Science and Services Division, at 1-800-662-9278 or visit our homepage at http://www.michigan.gov/deq.

PERMIT NO. M10005827 Page 19 of 26 PART II (0-A- J-7o ""

P Section B. Monitoring Procedures

1. Representative Samples Samples and measurements taken as required herein shall be representative of the volume and nature of the monitored discharge.

2. Test Procedures Test procedures for the analysis of pollutants shall conform to regulations promulgated pursuant to Section 304(h) of the Federal Act (40 CFR Part 136 - Guidelines Establishing Test Procedures for the Analysis of Pollutants), unless specified otherwise in this permit. Requests to use test procedures not promulgated under 40 CFR Part 136 for pollutant monitoring required by this permit shall be made in accordance with the Alternate Test Procedures regulations specified in 40 CFR 136.4. These requests shall be submitted to the Chief of the Permits Section, Water Bureau, Michigan Department of Environmental Quality, P.O. Box 30273, Lansing, Michigan, 48909-7773. The permittee may use such procedures upon appr6val.

The permittee shall periodically calibrate and perform maintenance procedures on all analytical instrumentation at intervals to ensure accuracy of measurements. The calibration and maintenance shall be performed as part of the permittee's laboratory Quality Control/Quality Assurance program.

3. Instrumentation The permittee shall periodically calibrate and perform maintenance procedures on all monitoring instrumentation at intervals to ensure accuracy of measurements.

4. Recording Results For each measurement or sample taken pursuant to the requirements of this permit, the permittee shall record the following information: 1) the exact place, date, and time of measurement or sampling; 2) the person(s) who performed the measurement or sample collection; 3) the dates the analyses were performed; 4) the person(s) who performed the analyses;

5) the analytical techniques or methods used; 6) the date of and person responsible for equipment calibration; and 7) the results of all required analyses.

5. Records Retention All records and information resulting from the monitoring activities required by this permit including all records of analyses performed and calibration and maintenance of instrumentation and recordings from continuous monitoring instrumentation shall be retained for a minimum of three (3) years, or longer if requested by the Regional Administrator or the Department.

PERMIT NO. M10005827 Page 20 of 26 PART II (0 Section C. Reporting Requirements I

1. Start-up Notification If the permittee will not discharge during the first 60 days following the effective date of this permit, the.permittee shall notify the Departmentwithin 14 days following the effective date of this permit, and then 60 days prior to the commencement of the discharge.

2. Submittal Requirements for Self-MonitoringData Unless instructed on the effluent limits page to conduct "retained self-monitoring," the permittee shall submnit self-monitoring data on the Environmental Protection Agency's Discharge Monitoring Report (DMR) forms (monthly summary information) and the Department's Daily Discharge Monitoring Report forms (daily information) to PCS-Data Entry, Water Bureau, Michigan Department of Environmental Quality, P.O. Box 30273, Lansing, Michigan, 48909-7773, for each calendar month of the authorized discharge period(s). The forms shall be postmarked no later than the 1 0 th day of the month following each month of the authorized discharge period(s). Electronic Environmental Discharge Monitoring Reporting (e2-DMR) System participants shall submit self-monitoring data for each month of the authorized discharge period(s). The electronic forms shall be submitted to the department no later than the 20th day of the month following each month of the authorized discharge period(s).

Alternative Daily Discharge Monitoring Report formats may be used if they provide equivalent reporting details and are approved by the Department. For information on the electronic submittal of this information, contact the Department or visit the e 2-Reporting website @ https://secureI.state.mi.us/e2rs/ - click on "about e-DMR" to download the Facility Participation Package.

3. Retained Self-Monitoring Requirements If instructed on the effluent limits page to conduct retained self-monitoring, the permittee shall maintain a year-to-date 10g of retained self-monitoring results and, upon request, provide such log for inspection to the staff of the Water Bureau, Michigan Department of Environmental Quality (in the case of hospitals, nursing homes and extended care facilities, to the staff of the Division of Health Facilities and Services, Michigan Department of Consumer and Industry Services). Retained self-monitoring results are public information and shall be promptly provided to the public upon request.

The permittee shall certify, in writing, to the Department, on or before January 10th of each year, that: 1) all retained self-monitoring requirements have been complied with arid a year-to-date log has been maintained; and 2) the application on which this permit is based still accurately describes the discharge.

4. Additional Monitoring by Permittee If the permittee monitors any pollutant at the location(s) designated herein more frequently than required by this permit, using approved analytical methods as specified above, the results of such monitoring shall be included in the calculation and reporting of the values required in the Discharge Monitoring Report. Such increased frequency shall also be indicated.

Monitoring required pursuant to Part 41 of the Michigan Act or Rule 35 of the Mobile Home Park Commission Act (Act 96 of the Public Acts of 1987) for assurance of proper facility operation shall be submitted as required by the Department.

5. Compliance Dates Notification Within 14 days of every compliance date specified in this permit, the permittee shall submit a written notification to the Department indicating whether or not the particular requirement was accomplished. If the requirement was not accomplished, the notification shall include an explanation of the failure to-accomplish the requirement, actions taken or planned by the permittee to correct the situation, and an estimate of when the requirement will be accomplished. If a written report is required to be submitted by a specified date and the permittee accomplishes this, a separate written notification is not required.

PERMIT NO. M10005827 Page 21 of 26 PART II (1O-t-1o Section C. Reporting Requirements

6. Noncompliance Notification Compliance with all applicable requirements set forth in the Federal Act, Parts 31 and 41 of the Michigan Act, and related regulations and rules is required. All instances of noncompliance shall be reported as follows:

a. 24-hour reporting - Any noncompliance which may endanger health or the environment (including maximum daily concentration discharge limitation exceedances) shall be reported, verbally, within 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> from the time the permittee becomes aware of the noncompliance. A written submission shall also be provided within five (5) days.

b. other reporting - The permittee shall report, in writing, all other instances of noncompliance not described in a.

above at the time monitoring reports are submitted; or, in the case of retained self-monitoring, within five (5) days from the time the permittee becomes aware of the noncompliance.

Written reporting shall include: 1) a description of the discharge and cause of noncompliance; and 2) the period of noncompliance, including exact dates and times; or, if not corrected, the anticipated time the noncompliance is expected to continue, and the steps taken to reduce, eliminate and prevent recurrence of the noncomplying discharge.

7. Spill Notification The permittee shall immediately report any release of any polluting material which occurs to the surface waters or groundwaters of the state, unless the permittee has determined that the release is not in excess of the threshold reporting quantities specified in the Part 5 Rules (Rules 324.2001 through 324.2009 of the Michigan Administrative Code),'by calling the Department at the number indicated on the first page of this permit, or if the notice is provided after regular working hours call the Department's 24-hour Pollution Emergency Alerting System telephone number, 1-800-292-4706 (calls from out-of-state dial 1-517-373-7660).

Within ten (10) days of the release, the permittee shall submit to the Department a full written explanation as to the cause of the release, the discovery of the release, response (clean-up and/or recovery) measures taken, and preventative measures taken or a schedule for completion of measures to be taken to prevent reoccurrence of similar releases.

8. Upset Noncompliance Notification If a process "upset" (defined as an exceptional incident in which there is unintentional and temporary noncompliance with technology based permit effluent limitations because of factors beyond the reasonable control of the permittee) has occurred, the permittee who wishes to establish the affirmative defense of upset, shall notify the Department by telephone within 24-hours of becoming aware of such conditions; and within five (5) days, provide in writing, the following information:

a. that an upset occurred and that the permittee can identify the specific cause(s) of the upset;

b. that the permitted wastewater treatment facility was, at the time, being properly operated; and

c. that the permittee has specified and taken action on all responsible steps to minimize or correct any adverse impact in the environment resulting from noncompliance with this permit.

In any enforcement proceedings, the permittee, seeking to establish the occurrence of an upset, has the burden of proof.

PERMIT NO. M10005827 Page 22 of 26 PART II Section C. Reporting Requirements v .

9. Bypass Prohibition and Notification

a. Bypass Prohibition - Bypass is prohibited unless:

1) bypass was unavoidable to prevent loss of life, personal injury, or severe property damage;

2) there were no feasible alternatives to the bypass, such as the use of auxiliary treatment facilities, retention of untreated wastes, or maintenance during normal periods of equipment downtime. This condition is not satisfied if adequate backup equipment should have been installed in the exercise of reasonable engineering judgment to prevent a bypass; and

3) the permittee submitted notices as required under 9.b. or 9.c. below.

b. Notice of Anticipated Bypass - If the permittee knows in advance of the need for a .bypass, it shall submit prior notice to the Department, if possible at least ten (10) days before the date of the bypass, and provide information about the anticipated bypass as required by the Department. The Department may approve an anticipated bypass, after considering its adverse effects, if it will meet the three (3) conditions listed in 9.a. above.

c. Notice of Unanticipated Bypass - The permittee shall submit notice to the Department of an unanticipated bypass.

by calling the Department at the number indicated on the first page of this permit (if the notice is provided after regular working hours, use the following number: 1-800-292-4706) as soon as possible, but no later than 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> from the time the permittee becomes aware of the circumstances.

d. Written Report of Bypass - A written submission shall be provided within five (5) working days of commencing any bypass to the Department, and at additional times as directed by the Department. The written submission shall contain a description of the bypass and its cause; the period of bypass, including exact dates and times, and if the bypass has not been corrected, the anticipated time it is expected to continue; steps taken or planned to reduce, eliminate, and prevent reoccurrence of the bypass; and other information as required by the Department.

e. Bypass Not Exceeding Limitations - The permittee may allow any bypass to occur which does not cause effluent limitations to be exceeded, but only if it also is for essential maintenance to assure efficient operation. *These bypasses are not subject to the provisions of 9.a., 9.b., 9.c., and 9.d., above. This provision does not relieve the permittee of any notification responsibilities under Part II.C. 10. of this permit.

f Definitions

1) Bypass means the intentional diversion of waste streams from any portion of a treatment facility.

2) Severe property damage means substantial physical damage to property, damage to the treatment facilities which causes them to become inoperable, or substantial and permanent loss of natural resources which can reasonably be expected to occur in the absence of a bypass. Severe property damage does not mean economic loss caused by delays in production.

10. Notification of Changes in Discharge The permittee shall notify the Department, in writing, within 10 days of knowing, or having reason to believe, that any activity or change has occurred or will occur which would result in the discharge of: 1) detectable levels of chemicals on the current Michigan Critical Materials Register, priority pollutants or hazardous substances set forth in 40 CFR 122.21, Appendix D, or the Pollutants of Initial Focus in the Great Lakes Water Quality Initiative specified in 40 CFR 132.6, Table 6, which were not acknowledged in the application or listed in the application at less than detectable levels; 2) detectable levels of any other chemical not listed in the application or listed at less than detection, for which the application specifically requested information; or 3) any chemical at levels greater than five times the average level reported in the complete application (see the first page of this permit for the date(s) the complete application was submitted). Any other monitoring results obtained as a requirement of this permit shall be reported in accordance with the compliance schedules.

PERMIT NO. M10005827 Page 23 of 26 PART II -M1-- -

Section C. Reporting Requirements X

11. Changes in Facility Operations Any anticipated action or activity, including but not limited to facility expansion, production increases, or process modification, which will result in new or increased loadings of pollutants to the receiving waters must be reported to the Department by a) submission of an increased use request (application) and all information required under Rule 323.1098 (Antidegradation) of the Water Quality Standards or b) by notice if the following conditions are met: 1) the action or activity will not result in a change in the types of wastewater discharged or result in a greater quantity of wastewater than currently authorized by this permit; 2) the action or activity will not result in violations of the effluent limitations specified in this permit; 3) the action or activity is not prohibited by the requirements of Part II.C. 12.; and 4) the action or activity will not require notification pursuant to Part II.C.10. Following such notice, the permit may be modified according to applicable laws and rules to specify and limit any pollutant not previously limited.

12. Bioaccumulative Chemicals of Concern (BCC)

Consistent with the requirements of Rules 323.1098 and 323.1215 of the Michigan Administrative Code, the permittee is prohibited from undertaking any action that would result in a lowering of water quality fiom an increased loading ofa BCC unless an increased use request and antidegradation demonstration have been submitted and approved by the Department.

13. Transfer of Ownership or Control In the event of any change in control or ownership of facilities from which the authorized discharge emanates, the permittee shall submit to the Department 30 days prior to the actual transfer of ownership or control a written agreement between the current permittee and the new permittee containing: 1) the legal name and address of the new owner; 2) a specific date for the effective transfer of permit responsibility, coverage and liability; and 3) a certification of the continuity of or any changes in operations, wastewater discharge, or wastewater treatment.

If the new permittee is proposing changes in operations, wastewater discharge, or wastewater treatment, the Department may propose modification of this permit in accordance with applicable laws and rules.

PERMIT NO. M10005827 Z4- 26

.*,i.24of PART II Section D. Management Responsibilities

1. Duty to Comply All discharges authorized herein shall be consistent with the terms and conditions of this permit. The discharge of any pollutant identified in this permit more frequently than or at a level in excess of that authorized shall constitute a violation of the permit.

It is the duty of the permittee to comply with all the terms and conditions of this permit. Any noncompliance with the Effluent Limitations, Special Conditions, or terms of this permit constitutes a violation of the Michigan Act and/or the Federal Act and constitutes grounds for enforcement action; for permit termination, revocation and reissuance, or modification; or denial of an application for permit renewal.

2. Operator Certification The permittee shall have the waste treatment facilities under direct supervision of an operator certified at the appropriate level for the facility certification by the Department, as required by Sections 3110 and 4104 of the Michigan Act.

Permittees authorized to discharge storm water shall have the storm water treatment and/or control measures under direct supervision of a storm water operator certified by the Department, as required by Section 3110 of the Michigan Act.

3. Facilities Operation The permittee shall, at all times, properly operate and maintain all treatment or control facilities or systems installed or used by the permittee to achieve compliance with the terms and conditions of this permit. Proper operation and maintenance includes adequate laboratory controls and appropriate quality assurance procedures. -

4. Power Failures In order to maintain compliance with the effluent limitations of this permit and prevent unauthorized discharges, the permittee shall either:

a. provide an alternative power source sufficienf to operate facilities, utilized by the permittee to maintain compliance with the effluent limitations and conditions of this permit; or

b. upon the reduction, loss, or failure of one or more of the primary sources of power to facilities utilized by the permittee to -maintaincompliance with the effluent limitations and conditions of this permit, the permittee shall halt, reduce or otherwise control production and/or all discharge in order to maintain compliance with the effluent limitations and conditions of this permit.

5. Adverse Impact The permittee shall take all reasonable steps to minimize any adverse impact to the surface waters or. groundwaters of the state resulting from noncompliance with any effluent limitation specified in this permit including, but not limited to, such accelerated or additional monitoring as necessary to determine the nature and impact of the discharge in noncompliance.

6. Containment Facilities The permittee shall provide facilities for containment of any accidental losses of polluting materials in accordance with the requirements of the Part 5 Rules (Rules 324.2001 through 324.2009 of the Michigan Administrative Code). For a Publicly Owned Treatment Work (POTW), these facilities shall be approved under Part 41 of the Michigan Act.

PERMIT NO. M10005827 Page 25 of 26 PART II Section D. Management Responsibilities

7. Waste Treatment Residues Residuals (i.e. solids, sludges, biosolids, filter backwash, scrubber water, ash, grit or other pollutants) removed from or resulting from treatment or control of wastewaters, shall be disposed of in an environmentally compatible manner and according to applicable laws and rules. These laws may include, but are not limited to, the Michigan Act, Part 31 for protection of water resources, Part 55 for air pollution control, Part I I I for hazardous waste management, Part 115 for solid waste management, Part 121 for liquid industrial wastes, Part 301 for protection of inland lakes and streams, and Part 303 for wetlands protection. Such disposal shall not result in any unlawful pollution of the air, surface waters or groundwaters of the state.

8. Treatment System Closure In the event that discharges from a treatment system are planned to be eliminated, the permittee shall submit a closure plan to the Department for approval. The closure plan shall include characterization of any wastewater and residuals which will remain on-site after the discharges are eliminated, along with disposal methods, proposed schedule, and any other relevant information as required by the Department. Closure activities involving waste treatment residuals shall be consistent with Part II.D.7. of this permit.

The permittee shall implement the closure activities in accordance with the approved plan. Any wastewater or residual disposal inconsistent with the approved plan shall be considered a violation of this permit. After proper closure of the treatment system, this permit may be terminated.

9. Right of Entry The permittee shall allow the Department, any agent appointed by the Department or the Regional Administrator, upon the presentation of credentials:

a. to enter upon the permittee's premises where an effluent source is located or in which any records are required to be kept under the terms and conditions of this permit;.and

b. at reasonable times to have access to and copy any records required to be kept under the terms and conditions of this permit; to inspect process facilities, treatment works, monitoring methods and equipment regulated or required under this permit; and to sample any discharge of pollutants.

10. Availability of Reports Except for data.determined to be confidential under Section 308 of the Federal Act and Rule 2128 (Rule 323.2128 of the Michigan Administrative Code), all reports prepared in accordance with the terms of this permit shall be available for public inspection at the offices of the Department and the Regional Administrator. As required by the Federal Act, effluent data shall not be considered confidential. Knowingly making any false statement on any such report may result in the imposition of criminal penalties as provided for in Section 309 of-the Federal Act and Sections 3 112, 3115, 4106 and 4110 of the Michigan Act.

PERMIT NO. Mi0005827. Page 26 of 26 PART 11 Section E. Activities Not Authorized by This Permit

1. Discharge to the Groundwaters This permit does not authorize any discharge to the groundwaters. Such discharge may be authorized by a groundwater discharge permit issued pursuant to the Michigan Act.

2. Facility Construction This permit does not authorize or approve the construction or modification of any physical structures or facilities.

Approval for such construction for a POTW must be by permit issued under Part 41 of the Michigan Act. Approval for such construction for a mobile home park, campground or marina shall be from the Water Bureau, Michigan Department of Environmental Quality. Approval for such construction for a hospital, nursing home or extended care facility shall be from the Division of Health Facilities and Services, Michigan Department of Consumer and Industry Services upon request.

3. Civil and Criminal Liability Except as provided in permit conditions on "Bypass" (Part II.C.9. pursuant to 40 CFR 122.4 1(m)), nothing in this permit shall be construed to relieve the permittee from civil or criminal penalties for noncompliance, whether or not such noncompliance is due to factors beyond the permittee's control, such as accidents, equipment breakdowns, or labor disputes.

4. Oil and Hazardous Substance Liability Nothing in this permit shall be construed to preclude the institution of any legal action or relieve the permittee from any responsibilities, liabilities, or penalties to which the permittee may be subject under Section 311 of the Federal Act except as are exempted by federal regulations.

5. State Laws Nothing in this permit shall be construed to preclude the institution of any legal action or relieve the permittee from any responsibilities, liabilities, or penalties established pursuant to any applicable state law or regulation under authority preserved by Section 510 of the Federal Act.

6. Property Rights The issuance of this permit does not convey any property rights in either real or personal property, or any exclusive privileges, nor does it authorize violation of any federal, state or local laws or regulations, nor does it obviate the necessity of obtaining such permits or approvals as may be required by law.

STATE OF MICHIGAN DEPARTMENT OF ENVIRONMENTAL QUALITY LANSING ENNIFER M. GRANHOLM STEVEN E. CHESTER GOVERNOR DIRECTOR December 13, 2007 Mr. Jon Harner Donald C. Cook Nuclear Plant One Cook Place Bridgman, Michigan 49106

SUBJECT:

National Pollutant Discharge Elimination System (NPDES); Permit No. M10005827 Designated Name: American Elec Power-Cook Pit Your National Pollutant Discharge Elimination System (NPDES) Permit has been processed in accordance with the appropriate state and federal regulations. It contains the requirements necessary for you to comply with state and federal water pollution control laws.

The issuance of this permit does not authorize the violation of any federal, state, or local laws or regulations, nor does it obviate the necessity of obtaining such permits, including any other Department of Environmental Quality (DEQ) permits, or approvals from other units of government as may be required by law.

REVIEW THE PERMIT EFFLUENT LIMITS AND COMPLIANCE SCHEDULES CAREFULLY.

These are subject to the criminal and civil enforcement provisions of both state and federal law.

Permit violations are audited by the DEQ and the United States Environmental Protection Agency (USEPA), and may appear in a published quarterly noncompliance report made available to agencies and the public.

Your monitoring and reporting responsibilities must be complied with in accordance with this permit. If required by the permit, self-monitoring data shall be reported via the Michigan DEQ Electronic Environmental Discharge Monitoring Reporting (e2-DMR) system. Other reports, notifications, or questions regarding the enclosed permit or the NPDES program should be directed to the following address:

Mr. Greg Danneffel, District Supervisor Kalamazoo District Office, Water Bureau, DEQ 7953 Adobe Road Kalamazoo, Michigan 49009-5026 Telephone: 269-567-3500; Fax: 269-567-9440 Sincerely, William Creal, Chief Permits Section Water Bureau Ib 517-241-1346 CONSTITUTION HALL - 525 WEST ALLEGAN STREET

• p.O. BOX 30273 - LANSING, MICHIGAN 48909-7773 www.michigan.gov * (517) 241-1300 f a .. \ a:

American Electric Power Company Donald C. Cook Nuclear Plant NPDES Permit No. M10005827 Page 2 wc/sea

Enclosure:

Permit No. M10005827 cc/enc: USEPA-Region 5 Mr. Gregory Danneffel, District Supervisor, Water Bureau (electronic)

PCS Unit, Water Bureau (permit only)

Files 69

ILMF[ IT NO. M10005827 MICHIGAN DEPARTMENT OF ENVIRONMENTAL QUALITY AUTHORIZATION TO DISCHARGE UNDER THE NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM In compliance with the provisions of the Federal Water Pollution Control Act, as amended, (33 U.S.C. 1251 et seq; the "Federal Act"), Michigan Act 451, Public Acts of 1994, as amended (the "Michigan Act"), Parts 31 and 41, aid Michigan Executive Orders 1991-31, 1995-4 and 1995-18, Indiana Michigan Power Company One Cook Place Bridgman, Michigan 49106 is authorized to discharge from the American Electric Power Company, Donald C. Cook Nuclear Plant, located at One Cook Place Bridgrnan, Michigan 49106 designated as American Elee Power-Cook Pit to the receiving water named Lake Michigan, in accordance with effluent limitations, monitoring requirements and other conditions set forth in this permit.

Unless specified otherwise, all contact with the Michigan Department of Environmental Quality (the '!Department") required by this permit shall be made to the Kalamazoo District Supervisor of the Water Bureau. The Kalamazoo District Office is located at 7953 Adobe Road, Kalamazoo, Michigan 49009-5026, telephone: 269-567-3500, fax: 269-567-9440. Unless specified otherwise, all Department approvals specified in this permit shall be by the District Supervisor.

In accordance with Section 324.3120 of the Michigan Act, the permittee shall make payment of an annual permit fee to the Department for each October 1 the permit is in effect regardless of occurrence of discharge. The permittee shall submit the fee in response to the Department's annual notice. The fee shall be postmarked by January 15 for notices mailed by December 1. The fee is due no later than 45 days after receiving the notice for notices mailed after December 1. Fees paid in accordance with the Michigan Act are not refundable.

In accordance with Section 324.3118 of the Michigan Act, the permittee shall make payment of an annual storm water fee to the Department for each January 1 the permit is in effect regardless of occurrence of discharge. The permittee shall submit the fee in response to the Department's annual notice. The fee shall be postmarked by March 15 for notices mailed by February 1. The fee is due no later than 45 days after receiving the notice for notices mailed after February 1. Fees paid in accordance with the Michigan Act are not refundable.

Any person who is aggrieved by this permit may file a sworn petition with the Office of Administrative Hearings of the Michigan Department of Labor and Economic Growth, setting forth the conditions of the permit which are being challenged and specifying the grounds for the challenge. The Department may reject any petition filed more than 60 days after issuance as being untimely.

This permit is based on a complete application submitted on April 1, 2003.

This modified permit takes effect immediately. The provisions of this permit are severable. After notice and opportunity for a hearing, this permit may be modified, suspended, or revoked in whole or in part during its term in accordance with applicable laws and rules. On its effective date this permit shall supersede NPDES Permit No. M10005827, expiring October 1, 2003, which is hereby revoked upon the effective date of this permit.

This permit and the authorization to discharge shall expire at midnight, October 1, 2008. In order to receive authorization to discharge beyond the date of expiration, the permittee shall submit an application which contains such information, forms, and fees as are required by the Department by April 4, 2008.

Issued September 24, 2004. Based on a request submitted on September 27, 2007, this permit was modified onr)pcnpmhpl' 11 2007 William Creal, Chief Permits Section Water Bureau 35

PERMIT NO. M10005827 Page 2 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements

1. Final Effluent Limitations, Monitoring Points 001A & 002A (Combined Discharge)

During the period beginning on the effective date of this permit and lasting until the expiration date of this permit, the pernittee is authorized to discharge a maximum of one billion five hundred million (1,500,000,000) gallons per day of noncontact condenser cooling water, miscellaneous low volume waters, and storm water runoff from Monitoring Point 001A through Outfall 001; and a maximum of one billion eight hundred twenty million (1,820,000,000) gallons per day of noncontact condenser cooling water, miscellaneous low volume waters, and storm water runoff from Monitoring Point 002A through Outfall 002. Outfalls 001 and 002 discharge to Lake Michigan. Such discharge shall be limited and monitored by the permittee as specified below.

Maximum Limits for Maximum Limits for Quantity or Loading Quality or Concentration Frequency Sample Parameter Monthly Daily Units Monthly Daily Units of Analysis Type Flow (report) (report) MGD ---.-.- .-.- Daily Report Total Daily Flow Total Residual Oxidant (TRO)

During Chlorination - No Bromine Use Discharge Mode Continuous (greater than 160 min/day) --- --- 38 ug/l 5X/Week Grab Intermittent (less than/equal to 160 min/day) --- --- 200 ug/l 5X/Week Grab During Bromine Use - the discharge time of bromine shall not exceed 120 mmin/day Intermittent (less than/equal to 120 min/day) --- .... 50 ug/l 5X/Week Grab TRO Discharge Time --- (report) min/day 5X/Week Report Total Discharge Time Temperature Intake --- (report) OF Daily Reading Discharge --- (report) OF Daily Reading Heat Additionh 17,300 MBTU/hr ---.-.- .-.- Daily Calculation Total Boroni --- (report) (report) ug/l Weekly Per Grab Occurrence Outfall Observation (report) ---

--- - Daily Visual Minimum Maximum Daily Daily pH 6.5 9.0 S.U. - Daily Grab

a. Narrative Standard The receiving water shall contain no unnatural turbidity, color, oil films, floating solids, foams, settleable solids, suspended solids, or deposits as a result of this discharge.

b. Monitoring Location Samples, measurements, and observations taken in compliance with the monitoring requirements above shall be taken prior to discharge to Lake Michigan.

q

PERMIT NO. M10005827 Page 3 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements

c. Outfall Observation Any unusual characteristics of the discharge (i.e., unnatural turbidity, color, oil filn, floating solids, foams, settleable solids, suspended solids, or deposits) shall be reported within 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> to the Department followed with a written report within five (5) days detailing the findings of the investigation and the steps taken to conrect the condition. For turbidity caused by the discharge of bentonite clay, the permittee is only required to verbally report to the Department.

d. Water Treatment Additives This permit does not authorize the discharge of water additives without approval from the Department. Approval of water additives is authorized under separate con-espondence. Water additives include any material that is added to water used at the facility or to a wastewater generated by the facility to condition or treat the water. In the event a permittee proposes to discharge water additives, including an increased discharge concentration of a previously approved water additive, the permittee shall submit a request to the Department for approval. See Part I.A.6. for information on requesting water treatment additive use.

e. TRO (Chlorine and Bromine) Requirements Total Residual Oxidant (TRO) shall be analyzed for using EPA Method 330.1 or Orion Electrode Model 97-70 (other analytical methods specified in 40 CFR 136 may be used upon approval of the Department). TRO monitoring is only required during periods of chlorine or bromine use and subsequent discharge. Limitations for the intermittent discharge of chlorine apply only when the discharge of chlorine is less than or equal to 160 minutes per day, otherwise the limitations for continuous discharge of chlorine apply. The intermittent discharge limitations shall apply when the service water system is chlorinated continuously and the noncontact condenser cooling water is chlorinated intermittently on the same day. Authorization to discharge bromine with or without chlorine is limited to 120 minutes per day at die limitations specified above with the additional requirement that any discharge of chlorine is restricted to a concun'ent discharge with bromine (no additional discharge of chlorine is authorized for that day).

During the intermittent discharge of chlorine without bromine ("During Chlorination - No Bromine Use" limitations given above), the daily concentration value reported for TRO shall be the average of a minimum of three (3) equally spaced grab samples taken during a chlorine discharge event, with the additional limitation that no single sample may exceed 300 .itg/l.

During the intermittent discharge of bromine with or without chlorine ("During Bromine Use" limitations given above), the daily concentration value reported for TRO shall be the maximum of at least three (3) equally spaced grab samples taken during a bromine discharge' event (no single sample may exceed 50 V.g/l).

The permittee shall enter a zero ("0") on the Discharge Monitoring Report for the TRO discharge modes not being used.

The permittee may use dehalogenation techniques to achieve the applicable TRO limitations, using sodium thiosulfate, sodium sulfite, sodium bisulfite, or other dehalogenating reagents approved by the Department. The quantity of reagent(s) used shall be limited to 1.5 times the stoichiometric amount of applied chlorine/brominie oxidant. Each month the permittee shall report the quantity of each dehalogenation reagent used per day.

f. Power Plants - PCB Prohibition Beginning upon the effective date of this permit, the permittee shall not discharge any polychlorinated biphenyls to the receiving waters of the state of Michigan as a result of plant operations other than due to the presence of such compounds in the intake water.

g. Chemical Metal Cleaning Wastes The permittee shall not discharge chemical metal cleaning wastes to the receiving waters of the state of Michigan as a result of plant operations.

h. Heat Addition The daily maximum limit of 17,300 MBTU/hr is for the total power plant discharge. The permittee shall report the total of heat loads discharged through Outfalls 001 and 002 under Outfall 001, in addition to reporting the heat loads discharged individually for the Outfalls 001 and 002, in the discharge monitoring reports.

PERMIT NO. M10005827 Page 4 or"26 PART I Section A. Effluent Limitations And Monitoring Requirements Total Boron Monitoring During ice melt operations, the pennittee shall sample the monitoring tanks for Total Boron prior to discharge through Outfalls 001 and 002. The permittee shall qiuantify the number of gallons released and perform a mass balance calculation and report the calculated boron discharged through Outfalls 001 and 002 in ug/l.

Analytical Method and Quantification Level for Total Boron The sampling procedures, preservation and handling, and analytical protocol for compliance monitoring for Total Boron shall be in accordance with EPA Method 200.7. The quantification level for Total Boron shall be 20 ug/l unless a higher level is appropriate because of sample matrix interference. Justification for higher quantification levels shall be submitted to the Department within 30 days of such determination. Upon approval of the Department, the permittee may use alternate analytical methods (for parameters with methods specified in 40 CFR 136, the alternate methods are restricted to those listed in 40 CFR 136).

I

PERM'IT NO. M [0005827 Page S of 26 PART I Section A. Effluent Limitations And Monitoring Requirements

2. Final Effluent Limitations, Monitoring Point OOA (Unit 1 Steam Generator Blowdown) and Monitoring Point OOB (Unit 2 Steam Generator Blowdown)

During the period beginning on the effective date of this permit and lasting until the expiration date of this permit, the permittee is authorized to discharge a maximum of one million (1,000,000) gallons per day of steam generator blowdown from Monitoring Point 00A through Outfalls 001 and 002; and one million (1,000,000) gallons per day of steam generator blowdown fr-om Monitoring Point 00B through outfalls 001 and 002. Such discharge shall be limited and monitored by the permittee as specified below.

Maximum Limits for Maximum Limits for Quantity or LoadinI4 Quality or Concentration Frequency Sample Pa ra meter Monthly DAL Units Monthl Daily Units of Analysis ,Type Flow (report) (report) MGD ---.-.- .-.- Daily Report Total Daily Flow Outfall Observation (report) --- Daily Visual

a. Monitoring Location Samples, measurements, and observations taken in compliance with the monitoring requirements above shall be taken at Monitoring Points OCA and 00B prior to discharge to the intake forebay and thence to Outfalls 001 or 002.

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VERM IT NO. M 10005827 Page 6 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements

3. Final Effluent Limitations, Monitoring Point OOC (Plant Heating Boiler Blowdown)

During the period beginning on the effective date of this permit and lasting until the expiration date of this permit, the permittee is authorized to discharge a maximum of forty three thousand (43,000) gallons per day of heating boiler blowdown from Monitoring Point QOC through Outfalls 001 and 002. Such discharge shall be limited and monitored by the pennittee as specified below.

Maximum Limits for Maximum Limits for Quantity or Loading Quality or Concentration Frequency Sample Parameter Monthly Daih' Units Monthly Da*ly Units of Analysis Type Flow (report) (report) MGD ---.-.- .-.- Daily Report 'otal Daily Flow Total Suspended Solidsb 30 100 mag/I Weekly Per Grab Occun'ence

a. Monitoring Location Samples and measurements taken in compliance with the monitoring requirements above shall be taken at Monitoring Point OOC prior to discharge to the intake forebay and thence to Outfalls 001 or 002,

b. Total Suspended Solids Total Suspended Solids are to be monitored daily per occurrence or weekly if the heating boiler is operated continuously for periods greater than one week.

PERMIT NO. M10005827 Pnge 7 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements

4. Final Effluent Limitations, Monitoring Point OOG (Reverse Osmosis System Reject)

During the period beginning on the effective date of this permit and lasting until the expiration date of this permit, the' pernittee is authorized to discharge a maximum of three hundred sixty six thousand (366,000) gallons per day of reverse osmosis system reject from Monitoring Point 00G through Outfalls 001 and 002. Such discharge shall be limited and monitored by the permittee as specified below.

Maximum Limits for Maximum Limits for Quantity or Loadine Quality or Concentration Frequency Sample Parameter Monthly Daily Units Monthly Daily Units of Analysis Type Flow (report) (report) MGD Daily Report Total Daily Flow Total Suspended Solids 30 100 mag/I Weekly Grab a, Monitoring Location Samples and measurements taken in compliance with the monitoring requirements above shall be taken at Monitoring Point OOG prior to discharge to the intake forebay and thence to Outfalls 001 and 002.

I PERMIT NO. M10005827 Page 8 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements

5. Final Effluent Limitations, Monitoring Point 00H (Turbine Room Sump Emergency Overflow)

During the period beginning on the effective date of this permit and lasting until the expiration date of this permit, the permittee is authorized to discharge a maximum of two million six hundred thousand (2,600,000) gallons per day of turbine room sump emergency overflow from Monitoring Point OOH through Outfalls 001 and 002. Such discharge shall be limited and monitored by the permittee as specified below.

Maximum Limits for Maximum Limits for Quantity or Loadin! Quality or Concentration Frequency Sample Parameter Monthly Daily Units Monthly Daily Units of Analysis Type Flow (report) (report) MGD Daily Per Report Total Occurrence Daily Flow (by Estimation)

Total Suspended Solids 30 100 mg/I 2X/Month Grab Per OccurTence Oil and Grease 15 20 mg/l 2X/Month Grab Per Occurrence a, Monitoring Location Samples and measurements taken in compliance with the monitoring requirements above shall be taken at Monitoring Point OOH prior to discharge to the intake forebay and thence to Outfalls 001 and 002.

b. Frequency of Analysis Samples and measurements shall be taken during discharge only.

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PERMIT NO. M10005827 Page 9 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements

6. Request for Discharge of Water Treatment Additives In the event a permittee proposes to discharge water additives, the permittee shall submit a request to discharge water additives to the Department for approval. Such requests shall be sent to the Surface Water Quality Assessment Section, Water Bureau, Department of Environmental Quality, P.O. Box 30273, Lansing, Michigan 48909, with a copy to the Department contact listed on-the cover page of this permit. Instructions to submit a request electronically may be obtained via the Internet (http://www.michigan.gov/deq and on the left side of the screen click on Water, Water Quality Monitoring, and Assessment of Michigan Waters; then click on the Water Treatment Additive List which is under the Information banner). Written approval fi-om the Department to discharge such additives at specified levels shall be obtained prior to discharge by the permittee. Additional monitoring and reporting may be required as a condition for the approval to discharge the additive.

A request to discharge water additives shall include all of the following water additive usage and discharge information:

a. Material Safety Data Sheet;

b. the proposed water additive discharge concentration;

c. the discharge frequency (i.e., number of hours per day and number of days per year);

d.. the monitoring point from which the product is to be discharged;

e. the type of removal treatment, if any, that the water additive receives prior to discharge;

f. product function (i.e. microbiocide, flocculant, etc.);

g. a 48-hour LC50 or EC5 0 for a North American freshwater planktonic crustacean (either Ceriodaphniasp., Daphnia sp., or Simocephalus sp.); and

h. the results of a toxicity test for one other North American freshwater aquatic species (other than a planktonic crustacean) that meets a minimum requirement of Rule 323.1057(2) of the Water Quality Standards.

Prior to submitting the request, the permittee may contact the Surface Water Quality Assessment Section by telephone at 517-335-1180 or via the Internet at the address given above to determine if the Department has the product toxicity data required by items g. and h. above. If the Department has the data, the permittee will not need to submit product toxicity data.

7. Temperature Modification The Department is considering the necessity of incorporating temperature limitations in this permit to assure thatthe requirements of Rule 323.1082 of the Water Quality Standards are met. Therefore, when consideration of this issue has been completed, the Department may modify this permit in accordance with applicable laws and rules to add appropriate temperature limitations or requirements.

8. Intake Screen Backwash, Outfall 004 During the period beginning on the effective date of this permit and lasting until the expiration date of this permit, the permittee is authorized to discharge intake screen backwash from outfall 004 to Lake Michigan. The permittee shall collect and remove debris accumulated on intaketrash bars and dispose of such material on land in an appropriate manner.

9. Deicing Discharge Authorization, Outfall 003 The pennittee is authorized to discharge a portion of the flow from outfall 001 or outfall 002 through intake structure outfall 003 to prevent ice buildup. The pennittee is not required to provide any additional monitoring of this discharge because the effluent limitations and monitoring requirements specified for outfalls 001 and 002 will determine compliance with applicable water quality standards and any other requirements.

PERMIT NO. M10005827 Page 10 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements

10. Storm Water Pollution Prevention Plan The permittee is authorized to discharge storm water associated with industrial activities as defined in 40 CFR 122.26(b)(14). These storm water discharges shall be controlled in accordance with the requirements of this special condition. The permittee has developed and implemented a Storm Water Pollution Prevention Plan (plan). The permittee shall continue implementation ofthe plan for maximum control of significant materials (as defined in Part I.A. 10.i.) so that storm water discharges will not cause a violation of the Water Quality Standards. The plan shall be routinely reviewed and updated in accordance with the requirements of this Special Condition.

a. Source Identification To identify potential sources of significant materials that can enter storm water and subsequently be discharged from the facility, the plan shall, at a minimum, include the following:

1) A site map identifying the following: buildings and other permanent structures; storage or disposal areas for significant materials; secondary containment structures; storm water discharge outfalls (numbered for reference); location of storm water inlets contributing to each outfall; location of NPDES permitted discharges other than storm water; outlines of thedrainage areas contributing to each outfall; structural runoff controls or storm water treatment facilities; areas of vegetation; areas of exposed and/or erodible soils; impervious surfaces (roofs, asphalt, concrete); name and location of receiving water(s); and areas of known or suspected impacts on surface waters as designated under Part 201 (Environmental Response) of the Michigan Act.

2) A list of all significant materials that could enter storm water. For each material listed, the plan shall include the following descriptions:

a) ways in which each type of material has been or has reasonable potential to become exposed to storm water (e.g., spillage during handling; leaks from pipes, pumps, and vessels; contact with storage piles; waste handling and disposal; deposits from dust or overspray, etc.);

b) identification of the outfall or outfalls through which the material may be discharged if released; c) a listing of spills and leaks of polluting materials in quantities reportable under the Part 5 Rules (Rules 324.2001 through 324.2009 of the Michigan Administrative Code) that occurred at areas that are exposed to precipitation or that othervise discharge to a point source at the facility. The listing shall include spills and leaks that occurred over the three (3) years prior to the completion of the plan or latest update of the plan; the date, volume and exact location of release; and the action taken to clean up the material and/or prevent exposure to storm water runoff or contamination of surface waters of the state. Any release that occurs after the plan has been developed shall be controlled in accordance with the plan and is cause for the plan to be updated as appropriate within 14 calendar days of obtaining knowledge~of the spill or loss; and d) a summary of existing storm water discharge sampling data (if available) describing pollutants in storm water discharges associated with industrial activity at the facility. This summary shall be accompanied by a description of the suspected source(s) of the pollutants detected.

3) An evaluation of the reasonable potential for contribution of significant materials to runoff from at least the following areas or activities: loading, unloading, and other material handling operations; outdoor storage, including secondary containment structures; outdoor manufacturing or processing activities; significant dust or particulate generating processes; discharge from vents, stacks and air emission controls; on-site waste disposal practices; maintenance and cleaning of vehicles, machines and equipment; sites of exposed and/or erodible soil; sites of environmental contamination listed under Part 201 (Environmental Response) of the Michigan Act; areas of significant material residue; and other areas where storm water may contact significant materials.

PERMIT NO. M10005827 Page I1I of 26 PART I Section A. Effluent Limitations And Monitoring Requirements

b. Preventive Measures and Source Controls, Non-Structural To prevent significant materials from contacting storm water at the source, the plan shall, at a minimum, include the following non-structural controls:

1) Description of a program for routine preventive maintenance which includes requirements for inspection and maintenance of storm water management and control devices (e.g., cleaning of oil/water separators and catch basins) as well as inspecting and testing plant equipment and systems to uncover conditions that could cause breakdowns or failures resulting in discharges of pollutants to surface waters. A log of the inspection and conrective actions shall be maintained on file by the permittee, and shall be retained in accordance with Part LA. 10.f.

2) A schedule for comprehensive site inspection to include visual inspection of equipment, pla.nt areas, and structural pollution prevention and treatment controls to be performed at least once every six (6) months. A report of the results of the comprehensive site inspection shall be prepared and retained in accordance with Part I.A. 10.f.

The report shall identify any incidents of non-compliance with the plan. If there are rio reportable incidents of non-compliance, the report shall contain a certification that the facility is in compliance with this plan.

3) A description of good housekeeping procedures to maintain a clean, orderly facility.

4) A description of material handling procedures and storage requirements for significant materials.

Equipment and procedures for cleaning up spills shall be identified in the plan and made available to the appropriate personnel. The procedures shall identify measures to prevent the spilled materials or material residues on the outside of containers from being discharged into storm water. The plan may include, by reference, requirements of either a Pollution Incident Prevention Plan (PIPP) prepared in accordance with the Part 5 Rules (Rules 324.2001 through 324.2009 of the Michigan Administrative Code); a Hazardous Waste Contingency Plan prepared in accordance with 40 CFR 264 and 265 Subpart D, as required by Part I l of the Michigan Act; or a Spill Prevention Control and Countermeasure (SPCC)plan prepared in accordance with 40 CFR 112.

5) Identification of areas that, due to topography, activities, or other factors, have a high potential for significant soil erosion. The plan shall also identify measures used to control soil erosion and sedimentation.

6) A description of employee training programs which will be implemented to inform appropriate personnel at all levels of responsibility of the components and goals of the plan. The plan shall identify periodic dates for such training.

7) Identification of significant materials expected to be present in storm water discharges following implementation of non-structuralpreventative measures and source controls.

e. Structural Controls for Prevention and Treatment Where implementation of the measures required by Part I.A. I0.b. does not control storm water discharges in accordance with Water Quality Standards in Part I.A.I0.h., the plan shall provide a description of the location, function, and design criteria of structural controls for prevention and treatment. Structural controls may be necessary:

I) to prevent uncontaminated storm water from contacting or being contacted by significant materials, and/or

2) if preventive measures are not feasible or are inadequate to keep significant materials at the site friom contaminating storm water. Structural controls shall be used to treat, divert, isolate, recycle, reuse or otherwise manage storm water in a manner that reduces the level of significant materials in the storm water and provides compliance with the Water Quality Standards in accordance with Part I.A.10.h.

d. Keeping Plans Current

1) The permittee shall review the plan on or before November 4 th of each year, and maintain written summaries of the reviews. Based on the review, the permittee shall amend the plan as needed to ensure continued compliance with the terms and conditions of this permit.

PERMIT NO. M10005827 Page 12 of"26 PART I Section A. Effluent Limitations And Monitoring Requirements

2) The plan shall also be updated or amended whenever changes or spills at the facility increase or have the potential to increase the exposure of significant materials to storm water, or when the'plan is determined by the permittee or the Department to be ineffective in achieving the general objectives of controlling pollutants in storm water discharges associated with industrial activity. Updates based on increased activity at the facility shall include a description of how the permittee intends to control any new sources of significant materials or respond to and prevent spills in accordance with the requirements of Parts I.A. 10.a., I.A. 1O.b., and I.A. 1O.c.

3) The Department or authorized representative may notify the permittee at any time that the plan does not meet minimum requirements. Such notification shall identify why the plan does not meet minimum requirements.

The permittee shall make the required changes to the plan within 30 days after such notification from the Department or authorized representative, and shall submit to the Department a written certification that the requested changes have been made.

e. Certified Storm Water Operator Update If the ceitified operator has changed or an additional certified storm water operator is added, the permittee shall provide the name and certification number of the new operator to the Department. The new operator shall review and sign the plan.

f. Signature and Plan Review

1) The plan shall be signed by the certified storm water operator and by either the permittee or an authorized representative in accordance with 40 CFR 122.22. The plan shall be retained on site of the facility that generates the storm water discharge..

2) The permittee shall make plans, reports, log books, runoff quality data, and s'upporting documents available upon request to the Department or authorized representative.

g. Record Keeping The penrnittee shall maintain records of all inspection and maintenance activities. Records shall also be kept describing incidents such as spills or other discharges that can affect the quality of storm water runoff. All such records shall be retained for three (3) years.

h. Water Quality Standards At the time of discharge, there shall be no violation of the Water Quality Standards in~the receiving waters as a result of this discharge, This requirement includes, but is not limited to, the following conditions:

1) In accordance with Rule 323.1050 of the Water Quality-Standards, the receiving waters shall not have any of the following unnatural physical properties in quantities which are or may become injurious to any designated use: unnatural turbidity, color, oil film, floating solids, foams, settleable solids, suspended solids, or deposits as a' result of this discharge.

2) Any unusual characteristics of the discharge (i.e., unnatural turbidity, color, oil film, floating solids, foams, settleable solids, suspended solids, or deposits) shall be reported within 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> to the Department followed with a written report within five (5) days detailing the findings of the investigation and the steps taken to correct the condition.

Significant Materials Significant Materials means any material which could degrade or impair water quality, including but not limited to:

raw materials; fuels; solvents, detergents, and plastic pellets; finished materials such a' metallic products; hazardous substances designated under Section 101(14) of Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) (see 40 CFR 372.65); any chemical the facility is required to report pursuant to Section 313 of Emergency Planning and Community Right-to-Know Act (EPCRA); polluting materials as identified under the Part 5 Rules (Rules 324.2001 through 324.2009 of the Michigan Administrative Code);

Hazardous Wastes as defined in Part Ill of the Michigan Act; fertilizers; pesticides; and waste products such as ashes, slag, and sludge that have the potential to be released with storm water discharges.

PERMIT NO. M10005827 Page 13 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements Prohibition of Non-storm Water Discharges Discharges of material other than storm water shall be in compliance with an NPDES permit issued for the discharge. Storm water shall be defined to include die following non-storm water discharges provided pollution prevention controls for the non-storm water component are identified in the plan: discharges firom fire hydrant flushing, potable water sources including water line flushing, fire system test water, irrigation drainage, lawn watering, routine building wash down which does not use detergents or other compounds, pavement wash water where spills or leaks of toxic or hazardous materials have not occurred (unless all spilled material have been removed) and where detergents arc not used, air conditioning condensate, springs, uncontaminated groundwater, and foundation or footing drains where flows are not contaminated with process materials such as solvents.

Discharges from fire fighting activities are authorized by this permit, but do not have to be identified in the plan.

11. Cooling Water Intake Structure This condition establishes the progn'am necessary for proceeding toward compliance with the regulations under Section 316(b) of the Clean Water Act. The purpose of these regulations is to establish the best technology available for minimizing adverse environmental impact associated with the use of cooling water intake structures.

The permittee submitted the Proposal for Information Collection on June 13, 2005. The permittee shall submit the information regarding Source Water Physical Data, Cooling Water Intake Structure Data, Cooling Water System Data, and hmpingement Mortality and/or Entrainment Characterization Study report on or before April 4, 2008. The permittee shall also submit the remaining applicable portions of the Comprehensive Demonstration Study upon notification by the Department. When notifying the permittee, the Department will allow the permittee reasonable amount of time in order to complete the necessary tasks.

Based on the review of the above information, the Department will determine appropriate requirements and conditions, such as specified below, to be included in the permit, either by modification or reissuance of the pennit.

a. Cooling water intake structure requirements,

b. Monitoring conditions,

c. Record keeping and reporting, and

d. Biennial status report.

PERNIr'r NO. M410005827 Page 14 of 26 PART I Section A. Effluent Limitations And Monitoring Requirements Facility Contact The "Facility Contact" was specified in the application. The permittee may replace tie facility contact at any time, and shall notify the Department in writing within 10 days after replacement (including the name, address and telephone number of the new facility contact),

a. The facility contact shall be (or a duly authorized representative of this person):

• for a corporation, a principal executive officer of at least the level of vice president, or a designated representative, if the representative is responsible for the overall operation of the facility from which the discharge described in the permit application or other NPDES form originates,

• for a partnership, a general partner,

• for a sole proprietorship, the proprietor, or

• for a municipal, state, or other public facility, either a principal executive officer, the mayor, village president, city or village manager or other duly authorized employee.

b. A person is a duly authorized representative only if:

0 the authorization is made in writing to the Department by a person described in paragraph a. of this section; and

• the authorization specifies either an individual or a position having responsibility for the overall operation of the regulated facility or activity such as the position of plant manager, operator ofa well or a well field, superintendent, position of equivalent responsibility, or an individual or position having overall responsibility for environmental matters for the facility (a duly authorized representative may thus be either a named individual or any individual occupying a named position).

Nothing in this section obviates the permittee from properly submitting reports and forms as required by law.

The Michigan Department of Environmental Quality (DEQ) encourages you to consider submitting your monthly Discharge Monitoring Reports (DMRs) electronically using the new web based Electronic Environmental Discharge Monitoring Reporting (e2-DMR) System. Explore the many benefits of this faster, more efficient alternative to submitting hand-written or paper-based Discharge Monitoring Reports. Participating in e2-Reporting will:

0 Improve Data Quality 0 Save Time 0l Reduce Compliance Costs 0 Better Utilize State Resources 0 Allow for Immediate Feedback 0 Improve Compliance Assessment As a fully operational electronic reporting system, all of the necessary legal, security, and electronic signature functionalities have been included for this system to serve as a completely paperless reporting system. A complete Facility ParticipationPackagewhich includes information regarding minimum system requirements and registration information can be found on the e2-DMR System Website. To access this document simply type in the following URL on the address line of your internet browser: https://securel .state. mi.us/e2rs/

Then click on "about e-DMR" to download the FacilityParticipationPackage.

PERMIT NO. MN10005827. Page IS of 26 PART II Section A. Definitions This list of definitions may include terms not applicable to this permit.

Acute toxic unit (TU.) means 100/LC5 0 where the LC 50 is determined from a whole effluent toxicity (WET) test which produces a result that is statistically or graphically estimated to be lethal to 50% of the test organisms.

Bioaccumulative chemical of concern (BCC) means a chemical which, upon entering the surface waters, by itself or as its toxic transformation product, accumulates in aquatic organisms by a human health bioaccumulation factor of more than 1000 after considering metabolism and other physiochemical properties that might enhance or inhibit bioaccumulation. The human health bioaccumulation factor shall be derived according to R 323.1057(5). Chemicals with half-lives of less than 8 weeks in the water column, sediment, and biota are not BCCs. The minimum bioaccumulation concentration factor (BAF) information needed to define an organic chemical as a BCC is either a field-measured BAF or a BAF derived using the biota-sediment accumulation factor (BSAF) methodology. The minimum BAF information needed to define an inorganic chemical as a BCC, including an organometal, is either a field-measured BAF or a laboratory-measured bioconcentration factor (BCF). The BCCs to which these rules apply are identified in table 5 of R 323.1057 of the Water Quality Standards.

Biosolids are the solid, semisolid, or liquid residues generated during the treatment of sanitary sewage or domestic sewage in a treatment works. This includes, but is not limited to, scum or solids removed in primary, secondary, or advanced wastewater treatment processes and a derivative of the removed scum or solids.

Bulk biosolids means biosolids that are not sold or given away in a bag or other container for application to a lawn or home garden.

Chronic toxic unit (TU,) means 100/MATC or 100/IC 25 , where the maximum acceptable toxicant concentration (MATC) and IC25 are expressed as a percent effluent in the test medium.

Class B Biosolids refers to material that has met the Class B pathogen reduction requirements or equivalent treatment by a Process to Significantly Reduce Pathogens (PSRP) in accordance with the Part 24 Rules. Processes include aerobic digestion, composting, anaerobic digestion, lime stabilization and air drying.

Daily concentration is the sum of the concentrations of the individual samples of a parameter divided by the number of samples taken during any calendar day. If the parameter concentration in any sample is less than the quantification limit, regard that value as zero when calculating the daily concentration. The daily concentration will be used to determine compliance with any maximum and minimum daily concentration limitations (except for pH and dissolved oxygen). When required by the permit, report the maximum calculated daily concentration for the month in the "MAXIMUM" column under "QUALITY OR CONCENTRATION" on the Discharge Monitoring Reports (DMRs).

For pH, report the maximum value of any individual sample taken during the month in the "MAXIMUM" column under "QUALITY OR CONCENTRATION" on the DMRs and the minimum value of any individual sample taken during the month in the "MINIMUM" column under "QUALITY OR CONCENTRATION" on the DMRs. For dissolved oxygen, report the minimum concentration of any individual sample in the "MINIMUM" column under "QUALITY OR CONCENTRATION" on the DMRs.

Daily loading is the total discharge by weight of a parameter discharged during any calendar day. This value is calculated by multiplying the daily concentration by the total daily flow and by the appropriate conversion factor. The daily loading will be used to determine compliance with any maximum daily loading limitations. When required by~the permit, report the maximum calculated daily loading for the month in the "MAXIMUM" column under "QUANTITY OR LOADING" on the DMRs.

Department means the Michigan Department of Environmental Quality.

Detection Level means the lowest concentration or amount of the target analyte that can be determined to be different from zero by a single measurement at a stated level of probability.

EC 50 means a statistically or graphically estimated concentration that is expected to cause I or more specified effects in 50% of a group of organisms under specified conditions.

PERMIT NO. M[0005827 Page 16 of 26 PART 1I Section A. Definitions Fecal coliform bacteria monthly is the geometric mean of the samples collected in a calendar month (or 30 consecutive days). The calculated monthly value will be used to determine compliance with the mnaxinmun monthly fecal coliform bacteria limitations. When required by the permit, report the calculated monthly value in the "AVERAGE" column under "QUALITY OR CONCENTRATION" on the DMRs.

Fecal coliform bacteria 7-day is the geometric mean of the samples collected in any 7-day period. The calculated 7-day value will be used to determine compliance with the maximum 7-day fecal coliform bacteria limitations. When required by the permit, report the maximum calculated 7-day concentration for the month in the "MAXIMUM" column under "QUALITY OR CONCENTRATION" on the DMRs.

Flow Proportioned sample is a composite sample with the sample volume proportional to the effluent flow.

Grab sample is a single sample taken at neither a set time nor flow.

IC25 means the toxicant concentration that would cause a 25% reduction in a nonquantal biological measurement for the test population.

Interference is a discharge which, alone or in conjunction with a discharge or discharges from other sources, both:

1) inhibits or disrupts the POTW, its treatment processes or operations, or its sludge processes, use or disposal; and 2)'.therefore, is a cause of a violation of any requirement of the POTW's NPDES permit (including an increase in the magnitude or duration of a violation) or, of the prevention of sewage sludge use or disposal in compliance with the following statutory provisions and regulations or permits issued thereunder (or more stringent state or local regulations):

Section 405 of the Clean Water Act, the Solid Waste Disposal Act (SWDA) (including Title II, more commonly referred to as the Resource Conservation and Recovery Act (RCRA), and including state regulations contained in any state sludge management plan prepared pursuant to Subtitle D of the SWDA), the Clean Air Act, the Toxic Substances Control Act, and the Marine Protection, Research and Sanctuaries Act. [This definition does not apply to sample matrix interference.]

Land Application means spraying or spreading biosolids or a biosolids derivative onto the land surface, injecting below the land surface, or incorporating into the soil so that the biosolids or biosolids derivative can either condition the soil or fertilize crops or vegetation grown in the soil.

LC5 0 means a statistically or graphically estimated concentration that is expected to be lethal to 50% of a group of organisms under specified conditions.

Maximum acceptable toxicant concentration (MATC) means the concentration obtained by calculating the geometric mean of the lower and upper chronic limits firom a chronic test. A-lower chronic limit is the highest tested concentration that did not cause the occurrence of a specific adverse effect. An upper chronic limit is the lowest tested concentration which did cause the occurrence of a specific adverse effect and above which all tested concentrations caused such an occurrence.

MGD means million gallons per day.

Monthly frequency of analysis refers to a calendar month. When required by this permit, an analytical result, reading, value or observation must be reported for that period if a discharge occurs during that period.

Monthly concentration is the sum of the daily concentrations determined during a reporting month (or 30 consecutive days) divided by the number of daily concentrations determined. The calculated monthly concentration will be used to deten-nine compliance with any maximum monthly concentration limitations. When required by the permit, report the calculated monthly concentration in the "AVERAGE" column under "QUALITY OR CONCENTRATION" on the DMRs.

For minimum percent removal requirements, the monthly influent concentration and the monthly effluent concentration shall be determined. The calculated monthly percent removal, which is equal to 100 times the quantity [I minus the quantity (monthly effluent concentration divided by the monthly influent concentration)], shall be reported in the "MINIMUM" column under "QUALITY OR CONCENTRATION" on the DMRs.

PERMIT NO. M10005827 Paget17of 26 PART II Section A. Definitions Monthly loading is the sum of the daily loadings ofa parameter divided by the number of daily loadings determined in the reporting month (or 30 consecutive days). The calculated monthly loading will be used to determine compliance with any maximum monthly loading limitations. When required by the permit, report the calculated monthly loading in the "AVERAGE" column under "QUANTITY OR LOADING" on the DMRs.

National Pretreatment Standards are the regulations promulgated by or to be promulgated by the Federal Environmental Protection Agency pursuant to Section 307(b) and (c) of the Federal Act. The standards establish nationwide limits for specific industrial categories for discharge to'a POTW.

NOAEL means the highest tested dose or concentration of a substance that results in no observed adverse effect in exposed test organisms where higher doses or concentrations result in an adverse effect.

Noneontact Cooling Water is water used for cooling which does not come into direct contact with any raw material, intermediate product, by-product, waste product or finished product. '

Nondomestic user is any discharger to a POTW that discharges wastes other than or in addition to water-carried wastes from toilet, kitchen, laundry, bathing or other facilities used for household purposes.

Pretreatment is reducing the amount of pollutants, eliminating pollutants, or altering the nature of pollutant properties to a less harmful state prior to discharge into a public sewer. The reduction or alteration can be by physical, chemical, or biological processes, process changes, or by other means. Dilution is not considered pretreatment unless expressly authorized by an applicable National Pretreatment Standard for a particular industrial category.

POTW is a publicly owned treatment works.

Quantification level means the measurement of the concentration of a contaminant obtained by using a specified laboratory procedure calculated at a specified concentration above the detection level. It is considered the lowest concentration at which a particular contaminant can be quantitatively measured using a specified laboratory procedure for monitoring of the contaminant.

Quarterly frequency of analysis refers to a three month period, defined as January through March, April through June, July through September, and October through December. When required by this permit, an analytical result, reading, value or observation must be reported for that period if a discharge occurs during that period.

Regional Administrator is the Region 5 Administrator, U.S. EPA, located at R-19J, 77 W. Jackson Blvd., Chicago, Illinois 60604.

Significant industrial user is a nondomestic user that: 1) is subject to Categorical Pretreatment Standards under 40 CFR 403.6 and 40 CFR Chapter 1, Subchapter N; or 2) discharges an average of 25,000 gallons per day or more of process wastewater to a POTW (excluding sanitary, noncontact cooling and boiler blowdown wastewater); contributes a process wastestream which makes up five (5) percent or more of the average dry weather hydraulic or organic capacity of the POTW treatment plant; or is designated as such by the permittee as defined in 40 CFR 403.12(a) on the basis that the industrial user has a reasonable potential for adversely affecting the POTW's treatment plant operation or violating any pretreatment standard or requirement (in accordance with 40 CFR 403.8(f)(6)).

Tier I value means a value for aquatic life, human health or Wildlife calculated under R 323.1057 of the Water Quality Standards using a tier I toxicity database.

Tier II value means a value for aquatic life, human health or wildlife calculated under R 323.1,057 of the Water Quality Standards using a tier II toxicity database.

Toxicity Redutction Evaluation (TRE) means a site-specific study conducted in a stepwise process designed to identify the causative agents of effluent toxicity, isolate the sources of toxicity, evaluate the effectiveness of toxicity control options, and then confirm the reduction in effluent toxicity.

PERMIT NO. M[0005827 Page IS of 26 PART II Section A. Definitions Water Quality Standards meansthe Part 4 Water Quality Standards promulgated pursuant to Part 31 of Act No. 451 of the Public Acts of 1994, as amended, being Rules 323.1041 through 323.1117.of the Michigan Administrative Code.

Weekly frequency of.analysis refers to a calendar week which begins oil Sunday and ends oil Saturday. When required by this permit, an analytical result, reading, value or observation must be reported for that period if a discharge occurs during that period.

Yearly frequency of analysis refers to a calendar year beginning on January 1 and ending on December 31. When required by this permit, an analytical result, reading, value or observation must be reported for that period if a discharge occurs during that period.

24-Hour Composite sample is a flow proportioned composite sample consisting of hourly or more fiequent portions that are taken over a 24-hour period.

3-Portion Composite sample is a sample consisting of three equal volume grab samples collected at equal intervals over an 8-hour period.

7-day concentration is the sum of the daily concentrations determined during any 7 consecutive days in a reporting month divided by the number of daily concentrations determined. The calculated 7-day concentration will be used to determine compliance with any maximum 7-day concentration limitations. When required by the permit, report the maximum calculated 7-day concentration for the month in the "MAXIMUM" column under "QUALITY OR CONCENTRATION" on the DMRs.

7-day loading is the sum of the daily loadings of a parameter divided by the number of daily loadings determined during any 7 consecutive days in a reporting month. The calculated 7-day loading will be used to determine compliance with any maximum 7-day loading limitations. When required by the permit, report the maximum calculated 7-day loading for the month in the "MAXIMUM" column under "QUANTITY OR LOADING" on the DMRs.

Preventing Pollution is the Best Solution The Michigan Department of Environmental Quality (DEQ) encourages you to consider pollution prevention alternatives. In some cases pollution prevention may allow you to avoid the need to discharge pollutants which would otherwise requirepermit limitations -- or even avoid the need for permits altogether! Pollution prevention can:

0 Save Money 0 Reduce Waste 0 Aid Permit Compliance 0 Protect Our Environment 0 Improve Corporate Image 0 Reduce Liability The DEQ is helping Michigan's industries save money, reduce waste and protect our environment through pollution prevention. DEQ staff can provide pollution prevention assistance through telephone' consultations, technical workshops and semninars, and informational publications. They can also put you directly in touch with local support networks and national pollution prevention resources. For more information, contact the Michigan Department of Environmental Quaiity, Environmental Science and Services Division, at 1-800-662-9278 or visit. our homepage at http://www.michigan.gov/deq.

PERMIT' NO. M10005827 Page 19 of 26 PART II Section B. Monitoring Procedures

1. Representative Samples Samples and measurements taken as required herein shall be representative of the volume and nature of the monitored discharge.

2. Test Procedures Test procedures for the analysis of pollutants shall conforni to regulations promulgated pursuant to Section 304(h) of the Federal Act (40 CFR Part 136 - Guidelines Establishing Test Procedures for the Analysis of Pollutants), unless specified otherwise in this permit. Requests to use test procedures not promulgated under 40 CFR Part 136 for: pollutant monitoring required by this permit shall be made in accordance with the Alternate Test Procedures regulations specified in 40 CFR 136.4. These requests shall be submitted to the Chief of the Permits Section, Water Bureau, Michigan Department of Environmental Quality, P.O. Box 30273, Lansing, Michigan, 48909-7773. The pennittee may use such procedures upon approval.

The permittee shall periodically calibrate and perform maintenance procedures on all analytical instrumentation at intervals to ensure accuracy of measurements. The calibration and maintenance shall be performed as part of the permittee's laboratory Quality Control/Quality Assurance program.

3. Instrumentation The permittee shall periodically calibrate and perform maintenance procedures on all monitoring instrumentation at intervals to ensure accuracy of measurements.

4. Recording Results For each measurement or sample taken pursuant to the requirements of this permit, the permittee shall record the following information: 1) the exact place, date, and time of measurement or sampling; 2) the person(s) who performed the measurement or sample collection; 3) the dates the analyses were performed; 4) the person(s) who performed the analyses;

5) the analytical techniques or methods used; 6) the date of and person responsible for equipment calibration; and 7) the results of all required analyses.

5. Records Retention All records and information resulting from the monitoring activities required by this permit including all records of analyses performed and calibration and maintenance of instrumentation and recordings from continuous monitoring instrumentation shall be retained for a minimum of three (3) years, or longer if requested by the Regional Administrator or the Department.

PERMIT NO. MI0005827 Page 20 of 26 PART II Section C. Reporting Requirements

1. Start-up Notification If the perinittee will not discharge during the first 60 days following the effective date of this permit, the permittee shall notify the Department within 14 days following the effective date of this permit, and then 60 days prior to the commencement of the discharge.

2. Submittal Requirements for Self-Monitoring Data Unless instructed on the effluent limits page to conduct "retained self-monitoring," the permittee shall subrrit self-monitoring data on the Environmental Protection Agency's Discharge Monitoring Report (DMR) forms (monthly summary information) and the Department's Daily Discharge Monitoring Report forms (daily information) to PCS-Data Entry, Water Bureau, Michigan Department of Environmental Quality, P.O. Box 30273, Lansing, Michigan,'48909-7773, for each calendar month of the authorized discharge period(s). The forms shall be postmarked no later than the 10"' day of the month following each month of the authorized discharge period(s). Electronic Environmental Discharge Monitoring Reporting (e2-DMR) System participants shall submit self-monitoring data for each month of the authorized discharge period(s). The electronic forms shall be submitted to the department no later than the 20th day of the month following each month of the authorized discharge period(s),

Alternative Daily Discharge Monitoring Report formats may be used if they provide equivalent reporting details and are approved by the Department. For information on the electronic submittal of this information, contact the Department or visit the e 2-Reporting website @ https://securel .state.mi.us/e2rs/ - click on "about e-DMR" to download the Facility Participation Package.

3. Retained Self-Monitoring Requirements If instructed on the effluent limits page to conduct retained self-monitoring, the permittee shall maintain a year-to-date log of retained self-monitoring results and, upon request, provide such log for inspection to the staff of the Water Bureau, Michigan Department of Environmental Quality (in the case of hospitals, nursing homes and extended care facilities, to the staff of the Division of Health Facilities and Services, Michigan Department of Consumer and Industry Services). Retained self-monitoring results are public information and shall be promptly provided to the public upon request.

The permittee shall certify, in writing, to the Department, on or before January 10th of each year, that: 1) all retained self-monitoring requirements have been complied with and a year-to-date log has been maintained; and 2) the application on which this permit is based still accurately describes the discharge.

4. Additional Monitoring by Permittee If the permittee monitors any pollutant at the location(s) designated herein more fi'equently than required by this permit, using approved analytical methods as specified above, the results of such monitoring shall be included in the calculation and reporting of the values required in the Discharge Monitoring Report. Such increased frequency shall also be indicated.

Monitoring required pursuant to Part 41 of the Michigan Act or Rule 35 of the Mobile Home Park Commission Act (Act 96 of the Public Acts of 1987) for assurance of proper facility operation shall be submitted as required by the Department.

5. Compliance Dates Notification Within 14 days of every compliance date specified in this permit, the permittee shall submit a written notification to the Department indicating whether or not the particular requirement was accomplished. If the requirement was not accomplished, the notification shall include an explanation of the failure to accomplish the requirement, actions taken or planned by the permittee to correct the situation, and an estimate of when the requirement will be accomplished. If a written report is required to be submitted by a specified date and the pernittee accomplishes this, a separate written notification is not required.

PERMIT NO. M10005827 Page 21 of 26 PART II Section C. Reporting Requirements

6. Noncompliance Notification Compliance with all applicable requirements set forth in the Federal Act, Parts 31 and 41 of the Michigan Act, and related regulations and rules is required. All instances of noncompliance shall be reported as follows:

a. 24-hour reporting - Any noncompliance which may endanger health or the environment (including maximum daily concentration discharge limitation exceedances) shall be reported, verbally, within 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> from the time the permittee becomes aware of the noncompliance. A written submission shall also be provided within five (5) days.

b. other reporting - The permittee shall report, in writing, all other instances of noncompliance not described in a.

above at the time monitoring reports are submitted; or, in the case of retained self-monitoring, within five (5) days from the time the permittee becomes aware of the noncompliance.

Written reporting shall include: 1) a description of the discharge and cause of noncompliance; and 2) the period of noncompliance, including exact dates and times; or, if not corrected, the anticipated time the noncompliance is expected to continue, and the steps taken to reduce, eliminate and prevent recurrence of the noncomplying discharge.

7. Spill Notification The permittee shall immediately report any release of any polluting material which occurs to the surface waters or groundwaters of the state, unless the permittee has determined that the release is not in excess of the threshold reporting quantities specified in the Part 5 Rules (Rules 324.2001 through 324.2009 of the Michigan Administrative Code), by calling the Department at the number indicated on the first page of this permit, or if the notice is provided after regular working hours call the Department's 24-hour Pollution Emergency Alerting System telephone number, 1-800-292-4706 (calls from out-of-state dial 1-517-373-7660).

Within ten (10) days of the release, the permittee shall submit to the Department a full written explanation as to the cause of the release, the discovery of the release, response (clean-up and/or recovery) measures taken, and preventative measures taken or a schedule for completion of measures to be taken to prevent reoccunrence of similar releases.

8. Upset Noncompliance Notification If a process "upset" (defined as an exceptional incident in which there is unintentional and temporary noncompliance with technology based permit effluent limitations because of factors beyond the reasonable control of the permittee) has occurred, the permittee who wishes to establish the affirmative defense of upset, shall notify the Department by telephone within 24-hours of becoming aware of such conditions; and within five (5) days, provide in writing, the following information:

a. that an upset occurred and that the permittee can identify the specific cause(s) of the upset;

b. that the permitted wastewater treatment facility was, at the time, being properly operated; and

c. that the permittee has specified and taken action on all responsible steps to minimize or correct any adverse impact in the environment resulting from noncompliance with this permit.

In amy enforcement proceedings, the permittee, seeking to establish the occurrence of an upset, has the burden of proof.

PERMIT NO. M[0005827 Page 22 of'26 PART II Section C. Reporting Requirements

9. Bypass Prohibition and Notification

a. Bypass Prohibition - Bypass is prohibited unless:

1) bypass was unavoidable to prevent loss of life, personal injury, or severe property damage;

2) there were no feasible alternatives to the bypass, such as the use of auxiliary treatment facilities, retention of untreated wastes, or maintenance during normal periods of equipment downtime. This condition is not satisfied if adequate backup equipment should have been installed in the exercise of reasonable engineering judgment to prevent a bypass; and

3) the permittee submitted notices as required under.9.b. or 9.c, below.

b. Notice of Anticipated Bypass - If the permittee knows in advance of the need for a bypass, it shall submit prior notice to the Department, if possible at least ten (10) days before the date of the bypass, and provide information about the anticipated bypass as required by the Department. The Department may approve an anticipated bypass, after considering its adverse effects, if it will meet the three (3) conditions listed in 9.a. above.

C. Notice of Unanticipated Bypass - The permittee shall subm it notice to the Department of an unanticipated bypass by calling the Department at the number indicated on the first page of this permit (if the notice is provided after regular working hours, use the following number: 1-800-292-4706) as soon as possible, but no later than 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> from the time the permittee becomes aware of the circumstances.

d. Written Report of Bypass - A written submission shall be provided within five (5) working days of commencing any bypass to the Department, and at additional times as directed by the Department. .The written submission shall contain a description of the bypass and its cause; the period of bypass, including exact dates and times, and if the bypass has not been corrected, the anticipated time it is expected to continue; steps taken or planned to reduce, eliminate, and prevent reoccurrence of the bypass; and other information as required by the Department.

e. Bypass Not Exceeding Limitations - The permittee may allow any bypass to occur which does not cause effluent limitations to be exceeded, but only if it also is for essential maintenance to assure efficient operation. These bypasses are not subject to the provisions of 9.a., 9.b., 9.c., and 9.d., above. This provision does not relieve the permittee of any notification responsibilities under Part I1.C. 10. of this permit.

f. Definitibns

1) Bypass means the intentional diversion of waste streams from any portion of a treatment facility.

2) Severe property damage means substantial physical damage to property, damage to the treatment facilities which causes them to become inoperable, or substantial and permanent loss of natural resources which can reasonably be expected to occur in the absence of a bypass. Severe property damage does not mean econornic loss caused by delays in production.

10. Notification of Changes in Discharge The permittee shall notify the Department, in writing, within 10 days of knowing, or having reason to believe, that any activity or change has occurred or will occur which would result in the discharge of. 1) detectable levels of chemicals on the current Michigan Critical Materials Register, priority pollutants or hazardous substances set forth in 40 CFR 122.21, Appendix D, or the Pollutants of Initial Focus in the Great Lakes Water Quality Initiative specified in 40 CFR 132.6, Table 6, which were not acknowledged in the application or listed in the application at less than detectable levels; 2) detectable levels of any other chemical not listed in the application or listed at less than detection, for which the application specifically requested information; or 3) any chemical at levels greater than five times the average level reported in the complete application (see the first page of this permit for the date(s) the complete application was submitted). Any other monitoring results obtained as a requirement of this permit shall be reported in accordance with the compliance schedules.

PERMIT NO. M10005827 Page 23 of 26 PART II Section C. Reporting Requirements

11. Changes in Facility Operations Any anticipated action or activity, including but not limited to facility expansion, production increases, or process modification, which will result in new or"increased loadings of pollutants to the receiving waters must be reported to the Department by a) submission of an increased use request (application) and all information required under Rule 323.1098 (Antidegradation) of the Water Quality Standards or b) by notice if the following conditions are met: 1) the action or activity will not result in a change in the types of wastewater discharged or result in a greater quantity of wastewater than currently authorized by this permit; 2) the action or activity will not result in violations of the effluent limitations specified in this permit; 3) the action or activity is not prohibited by the requirements of Part II.C. 12.; and 4) the action or activity will not require notification pursuant to Pa-t IIC.10. Following such notice, the permit may be modified according to applicable laws and rules to specify and limit any pollutant not previously limited.

12. Bioaccumulative Chemicals of Concern (BCC)

Consistent with the requirements of Rules 323.1098 and 323.1215 of the Michigan Administrative Code, the permittee is prohibited from undertaking any action that would result in a lowering of water quality from an increased loading of a BCC unless an increased use request and antidegradation demonstration have been submitted and approved by the Department.

13. Transfer of Ownership or Control In the event of any change in control or ownership of facilities from which the authorized discharge emanates, the permittee shall submit to the Department 30 days prior to the actual transfer of ownership or control a written agreement between the current permittee and the new permittee containing: 1)the legal name and address of the new owner; 2) a specific date for the effective transfer of permit responsibility, coverage and liability; and 3) a certification of the continuity of or any changes in operations, wastewater discharge, or wastewater treatment.

If the new permittee is proposing changes in operations, wastewater discharge, or wastewater treatment, the Department may propose modification of this permit in accordance with applicable laws and rules.

PERMIT NO. MI0005827 Page 24 of 26 PART 1I Section D. Management Responsibilities

1. Duty to Comply All discharges authorized herein shall be consistent with the terms and conditions of this permit. The discharge of any pollutant identified in this permit more frequently than or at a level in excess of that authorized shall constitute a violation of the permit.

It is the duty of the permittee to comply with all the terms and conditions of this permit. Any noncompliance with the Effluent Limitations, Special Conditions, or terms of this permit constitutes a violation of the Michigan Act and/or the Federal Act and constitutes grounds for enforcement action; for permit termination, revocation and reissuance, or modification; or denial of an application for permit renewal.

2. Operator Certification The permittee shall have the waste treatment facilities tinder direct supervision of an operator certified at the appropriate level for thefacility certification by the Department, as required by Sections 3 110 and 4104 of the Michigan Act.

Permittees authorized to discharge storm water shall have the storm water treatment and/or control measures under direct-supervision of a storm water operator certified by the Department, as required by Section 3110 of the Michigan Act.

3. Facilities Operation The permittee shall, at all times, properly operate and maintain all treatment or control facilities or systems installed or used by the permittee to achieve compliance with the terms and conditions of this permit. Proper operation and maintenance includes adequate laboratory controls and appropriate quality assurance procedures.

4. Power Failures In order to maintain compliance with the effluent limitations of this permit and prevent unauthorized discharges, the permittee shall either:

a. provide an alternative power source sufficient to operate facilities utilized by the permittee to maintain compliance with the effluent limitations and conditions of this permit; or

b. upon the reduction, loss, or failure of one or more of the primary sources of power to facilities utilized by the permittee to maintain compliance with the effluent limitations and conditions of this permit, the permittee shall halt, reduce or otherwise control production and/or all discharge in order to maintain compliance with the effluent limitations and conditions of this permit.

5. Adverse Impact The permittee shall take all reasonable steps to minimize any adverse impact to the surface waters or groundwaters of the state resulting from noncompliance with any effluent limitation specified in this permit including, but not limited to, such accelerated or additional monitoring as necessary to determine the nature and impact of the discharge in noncompliance.

6. Containment Facilities The pennittee shall provide facilities for containment of any accidental losses of polluting materials in accordance with the requirements of the Part 5 Rules (Rules 324.2001 through 324.2009 of the Michigan Administrative Code). For a Publicly Owned Treatment Work (POTW), these facilities shall be approved under Part 41 of the Michigan Act.

PERMIT NO. M"0005827 Vage 25 of 26 PART II Section D. Management Responsibilities

7. Waste Treatment Residues Residuals (i.e. solids, sludges, biosolids, filter backwash, scrubber water, ash, grit or other pollutants) removed from or resulting fi-orn treatment or control of wastewaters, shall be disposed of in an environmentally compatible manner and according to applicable laws and rules. These laws may include, but are not limited to, the Michigan Act, Part 31 for protection of water resources, Part 55 for air pollution control, Part Il1 for hazardous waste management, Part 115 for solid waste management, Part 121 for liquid industrial wastes, Part 301 for protection of inland lakes and streams, and Part 303 for wetlands protection. Such disposal shall not result in any unlawful pollution of the air, surface waters or groundwaters of the state.

8. Treatment System Closure In the event that discharges from a treatment system are planned to be eliminated, the permittee shall submit a closure plan to the Department for approval. The closure plan shall include characterization of any wastewater and residuals which will remain on-site after the discharges are eliminated, along with disposal methods, proposed schedule, andany other relevant information as required by the Department. Closure activities involving waste treatment residuals shall be consistent with Part IJ.D.7. of this permit.

The permittee shall implement the closure activities in accordance with the approved plan. Any wastewater or residual disposal inconsistent with the approved plan shall be considered a violation of this permit. After proper closure of the treatment system, this permit may be terminated.

9. Right of Entry The permittee shall allow the Department, any agent appointed by the Department or the Regional Administrator, upon the presentation of credentials:

a. to enter upon the permittee's premises where an effluent source is located or in which any records are required to be kept under the terms and conditions of this permit; and

b. at reasonable times to have access to and copy any records required to be kept under the terms and conditions of this permit; to inspect process facilities, treatment works, monitoring methods and equipment regulated or required under this permit; and to sample any discharge of pollutants.

10. Availability of Reports Except for data determined to be confidential under Section 308 of the Federal Act and Rule 2128 (Rule 323.2128 of the Michigan Administrative Code), all reports prepared in accordance with the terms of this permit shall be available for public inspection at the offices of the Department and the Regional Administrator. As required by the Federal Act, effluent data shall not be considered confidential. Knowingly making any false statement on any such report may result in the imposition of criminal penalties as provided for in Section 309 of the Federal Act and Sections 3112, 3115, 4106 and 4110 of the Michigan Act.

Pj)

P1ER4IT NO. M10005827 Page 26 of 26 PART II Section E. Activities Not Authorized by This Permit

1. Discharge to the Groundwaters This permit does not authorize any discharge to the groundwatcrs. Such discharge may be authorized by a groundwater discharge permit issued pursuant to the Michigan Act.

2. Facility Construction This permit does not authorize or approve the construction or modification of any physical structures or facilities.

Approval for such construction for a POTW must be by permait issued under Part 41 of the Michigan Act. Approval for such construction for a mobile home park, campground or marina shall be from the Water Bureau, Michigan Department of Environmental Quality. Approval for such construction for a hospital, nulsing home or extended care facility shall be friom the Division of Health Facilities and Services, Michigan Department of Consumer and Industry Services upon request.

3. Civil and Criminal Liability Except as provided in permit conditions on "Bypass" (Part II.C.9, pursuant to 40 CFR 122.41(m)), nothing in this permit shall be construed to relieve the permittee from civil or criminal penalties for noncompliance, whether or not such noncompliance is due to factors beyond the permittee's control, such as accidents, equipment breakdowns, or labor disputes.

4. Oil and Hazardous Substance Liability Nothing in this permit shall be construed to preclude the institution of any legal action or relieve the permittee from any responsibilities, liabilities, or penalties to which the permittee may be subject under Section 311 of the Federal Act except as are exempted by federal regulations.

5. State Laws Nothing in this permit shall be construed to preclude the institution of any legal action or relieve the permittee from any responsibilities, liabilities, or penalties established pursuant to any applicable state law or regulation under authority preserved by Section 510 of the Federal Act.

6. Property Rights The issuance of this permit does not convey any property rights in either real or personal property, or any exclusive privileges, nor does it authorize violation of any federal, state or local laws or regulations, nor does it obviate the necessity of obtaining such permits or approvals as may be required by law.

Indiana Michigan Power Cook Nuclear Plant OneCook Place BridgmanMl 4910B AEP.com A unit ofAmerican Electric Power Mr. James Entzminger (SC-6J)

Office of Chemical Emergency Preparedness and Prevention U. S. Environmental Protection Agency 77 West Jackson Blvd.

• Chicago, IL 60604-3511 June 8, 2007

Subject:

Matter of Indiana Michigan Power Company, d/b/a American Electric Power Cook Nuclear Plant, Bridgman, Michigan Docket Number CERCLA-05-2004-001 0, EPCRA-05-2004-0043, MM-05-2004-0003

Dear Mr. Entzminger:

This interim report is submitted in accordance with Paragraph 22 of the Consent Agreement and Final Order, (CAFO) in the referenced matter to address the status of the supplemental environmental project (SEP) and associated costs. -

Indiana Michigan Power Company has maintained the SEP at Cook Nuclear Plant for the year 2006.

• The new double-walled feed piping for the plant sodium hypochlorite system, improved leak detection, and a new sodium hypochlorite pump room with improved secondary containment remains in operation since its installation on May 11, 2006.

The actual costs,,ia ed. for, purchasing and installing the described SEP were $730,827.10. The actual costs incurred for purchase, installation, operation, and maintenance of the SEP thus far are

$848,427.68. These SEP costs exceed the minimum amount required by Paragraph 17 of the CAFO.

Should you have any questions, please contact Mr. Jon H. Harner, Environmental Manager, at (269) 465-5901, extension 2102.

Sincerely,

?seph N. Jensen

- Site. Vice President JEN/rdw Correspondence Control #2007-512

Mr. James Entzminger CC #2007-512 Page 2 June 8, 2007 I certify that I am familiar with the information in this document and that, based on an inquiry of those individuals responsible for obtaining the information, the information is true and complete to the best of my knowledge. I understand there are significant penalties for submitting false information, including the possibility of fines and imprisonment for knowing violations.

. Jensen President

Mr-. James Entzrriinger CC.#2007-512 Page 3 June 8, 2007 c: K. D. Curry - AEP, Ft. Wayne J. T. King - MPSC K. D. Mack - AEPHQ, Columbus MDEQ - WHMD/RPMWS

APPENDIX V SPECIAL REPORTS 2007

SECTION 316(b), PHASE II FISH IMPINGEMENT MORTALITY AND ENTRAINMENT CHARACTERIZATION STUDY AT THE DONALD C. COOK NUCLEAR PLANT 2005-2007 OCTOBER 2007 NORMANDEAU ASSOCIATES ENVIRONMENTAL CONSULTANTS 4e- 0~

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SECTION 316(b), PHASE II FISH IMPINGEMENT MORTALITY AND ENTRAINMENT CHARACTERIZATION STUDY AT THE DONALD C. COOK NUCLEAR PLANT 2005-2007 Preparedfor AMERICAN ELECTRIC POWER Cook Nuclear Plant One Cook Place Bridgman, MI 49106 Preparedby NORMANDEAU ASSOCIATES, INC.

25 Nashua Road Bedford, NH 03110 R-20452.000 October 2007

316(b) PHASE It BASELINE FISH E & I STUDY Table of Contents Page

1.0 INTRODUCTION

............................................................................................................. 1 2.0 METHODS AND M ATERIALS .................................................................................... 2 2.1 IMPINGEMENT FIELD PROCEDURES ............................................................................ 2 2.2 ENTRAINM ENT METHODS ............................................................................................. 2 2.2.1 Ichthyoplankton (Entrainment and Nearfield Sampling) Laboratory Processing Procedures ....................................... 3 2.3 NEARFIELD SAMPLING ...................................................................................................... 3 2.3.1 Ichthyoplankton Sampling ............................................................................ 4 2.3.2 Gill Net Sampling ........................................................................................... 5 2.3.3 Otter Trawl Sampling ....................................................................................... 5 2.3.4 Seine Sampling ................................................. 5 2.4 ANALYTIC M ETHODS ................................................................................................. 6 2.4.1 Entrainm ent Analytic M ethods ..................................................................... 6 2.4.2 Impingement Analytic M ethods ........ ...........................................................

6 2.4.3 Nearfield Sampling Analytic Methods ......................................................... 6 2.5 QUALITY ASSURANCE AND CONTROL (QA/QC) METHODS AND SAMPLE CHAIN OF CUSTODY .......................................................................................................................... 7 2.5.1 Tasks Subject to Quality Control .................................................................. 7 2.5.2 Inspection Plans ........................................... 7 2.5.3 Acceptance/Rejection Criteria ....................................................................... 8 2.5.4 Quality Control Records ................................................................................. 9 2.5.5 Reference Collection ...................................................................................... 9 3.0 RESULTS ............................................................................................................................... 10 3.1 ENTRAINMENT ................................................................................................................ 10 3.2 IMPINGEMENT ................................................................................................................. 17 3 .2 .1 - U n it I ................................................................................ ................................. 17 3 .2 .2 Unit 2 ............................................................................................ .................... 2 2 3.3 NEARFIELD SAMPLING .............................................................................................. 29 3.3.1 Ichthyoplankton Sampling .......................................................................... 29 3.3.2 Gill N et Sampling ........................................................................................ 36 3.3.3 Otter Trawl Sampling ................................................................................... 37 3.3.4 Seine Sampling ............................................................................................ 45 3.4 WATER QUALITY ............................................... 47 3.4.1 Ichthyoplankton Water Quality Sampling ................................................... 47 3.4.2 Gill Net W ater Quality Sampling ................................................................ 47 3.4.3 Otter Trawl W ater Quality Sampling ......................................................... 47 3.4.4 Seine Water Quality Sampling ............................................. 48 3.5 QUALITY ASSURANCE AND QUALITY CONTROL (QA/QC) RESULTS ...................... 48 4.0 DISCUSSION .......................................................................................................................... 49 5.0 LITERATURE CITED ................................................................................................... 52 APPENDIX 20452 Cook 316b Baseline Final.doc 10/4/07 ii Normandeau Associates, Inc.

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316(b) PHASE I BASELINE FISH E & I STUDY List of Figures Page Figure 2-1. Location of sampling stations in the nearfield area in the vicinity of Cook N u clear P lan t......................................................................................................................... 4 Figure 3-1. Estimated total entrainment (in millions) of fish eggs, larvae, and young-of-the-year and older fish at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007 ...................................................................... 11 Figure 3-2. Estimated species composition of fish eggs entrained (in millions) by month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007 ........................... .......................................................................... 12 Figure 3-3. Estimated species composition of fish larvae entrained (in millions) by month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007. ............................................. 13 Figure 3-4. Estimated species composition of young-of-the-year and older fish larvae entrained (in millions) by month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007 ......................... 14 Figure 3-5. Estimated total number of fish impinged and species composition by month at Unit I of Cook Nuclear Power Place February 2006 through January 2007 ............... 20 Figure 3-6. Estimated biomass (kg) of fish impinged and biomass composition by month at Unit 1 of Cook Nuclear Plant February 2006 through January 2007 ........................... 23 Figure 3-7. Estimated total number of fish impinged and species composition by month at Unit 2 of Cook Nuclear Plant February 2006 through January 2007 ........................... 24 Figure 3-8. Estimated biomass (kg) of fish impinged and biomass composition by month at Unit 2 of Cook Nuclear Plant February 2006 through January 2007 ............................. 31 Figure 3-9. Mean Ichthyoplankton Density (No./100 M 3 ) at the Ichthyoplankton Sampling Stations in the Nearfield Area, April through November 2005 and 2006, off Cook Nuclear Plant .............................................. 31 Figure 3-10. Mean Catch per Unit Effort (fish/hour) at the Gill Net Sampling Stations in the Nearfield Area, April through November 2005 and 2006, off Cook Nuclear Plan t. ................................................................................................................................... 37 Figure 3-11. Mean Catch per Unit Effort (fish/trawl) at the Trawl Sampling Stations in the Nearfield Area, April through November 2005 and 2006, off Cook Nuclear Plant.......... .... ................................................. ........... ......... ............ 43 20452 Cook 316b Baseline Final.doc 10/4/07 111 NormandeauAssociates, Inc.

316(b) PHASE I! BASELINE FISH E & I STUDY List of Tables Page Table 3-1. Estimated number (in millions) of fish eggs, larvae, and young-of-year and older fish Entrained by Month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007 ............. ............... .............................. 11.

Table 3-2. Estimated number (in millions) of Fish Eggs Entrained by Month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through Januaiy 2007 ........................................................................................... 12 Table 3-3. Estimated number (in millions) of Fish Larvae Entrained by Month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007 ................................................................................................................ 13 Table 3-4. Estimated number (in millions) of Young-of-the-Year and Older Fish Entrained by Month at Cook Nuclear Power Plan Assuming Design Cooling Water Flow, February 2006 through January 2007 ........................................................................... 14 Table 3-5. Comparison of fish entrainment estimates (in millions) between July 2005 through January 2006 and July 2006 through January 2007 at Cook Nuclear Plan t ................................... ................................................................................................... 15 Table 3-6. Entrainment estimates (in millions) for each lifestage among the four diel periods sam pled at the Cook Nuclear Plant ................................................................................ 16 Table 3-7. Results of Analysis of Variance of Entrained Ichthyoplankton Lifestages at Cook Nuclear Plant.. Data are Log1o (x+l) transformed .............. * ......................................... 16 Table 3-8. Results of Least Squares Mean Multiple Comparisons Tests among Months for Monthly Egg Entrainment Estimates at Cook Nuclear Plant. Months marked with an x in the same row are not significantly different ............................................. 17 Table 379. Estimated Number of Fish Impinged, at Cook Nuclear Plant Unit 1, Assuming Design Cooling Water Flow, February 2006 through January 2007. ............. 18 Table 3-10. Results of Analysis of Variance of Impinged Fish at Cook Nuclear Plant Unit 1.

Data are Loglo (x+l) transformed ............................................... 20 Table 3-11. Results of Scheffe's Multiple Comparisons Test among Months for Total Impingement Estimates at Cook Nuclear Plant Unit 1. Months marked with an x in the same row are not significantly different ............................................................ 20 Table 3-12. Results of Analysis of Variance of Yellow Perch Impingement at Cook Nuclear Plant Unit 1. Data are Loglo (x+l) transformed .......................................................... 21 Table 3-13. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Yellow Perch at Cook Nuclear Plant Unit 1. Months marked with an x in the sam e row are not significantly different ............................................................................. 21 20452 Cook 316b Baseline Final.doc 10/4/07 iv NormandeauAssociates, Inc.

316(b) PHASE ! BASELINE FISH E & I STUDY Table 3-14. Results of Analysis of Variance of Alewife Impingement at Cook Nuclear Plant Unit 1. D ata are Loglo (x+l) transform ed......................................... ..................... .......... 21 Table 3-15. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Alewife at Cook Nuclear Plant Unit 1. Months marked with an x in the same row are not significantly different .......................................................... 21 Table 3-16. Results of Analysis of Variancd of Spottail Shiner Impingement at Cook Nuclear

,Plant Unit 1. Data are Loglo (x+l) transformed .......................................................... 21 Table 3-17. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Spottail Shiner at Cook Nuclear Plant Unit 1. Months marked with an x in the sam e row are not significantly different ..................................................................... 22 Table 3-18. Comparison of fish impingement estimates between July 2005 through January 2006 and July 2006 through January 2007 at Cook Nuclear Plant Unit 1................... 23 Table 3-19. Estimated Number of Fish Impinged, at Cook Nuclear Plant Unit 2, Assuming Design Cooling Water Flow, February 2006 through January 2007 ................................. 25 Table 3-20. Results of Analysis of Variance of Impinged Fish at Cook Nuclear Plant Unit 2.

Data are Loglo (x+ l) transform ed ...................................................................................... 27 Table 3-21. Results of Scheffe's Multiple Comparisons Test among Months for Total Impingement Estimates at Cook Nuclear Plant Unit 2. Months marked with an x in the same row are not significantly different............................................................. 27 Table 3-22. Results of Analysis of Variance of Impinged Yellow Perch at Cook Nuclear Plant Unit 2. Data are Loglo (x+l) transformed .......................................................... 27 Table 3-23. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Yellow Perch at Cook Nuclear Plant Unit 2. Months marked with an x in the same row are not significantly different ................................................... 27 Table 3-24. Results of Analysis of Variance of Impinged Spottail Shiner at Cook Nuclear Plant Unit 2. Data are Loglo (x+l) transformed .......................................................... 28 Table 3-25. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Spottail Shiner at Cook Nuclear Plant Unit 2. Months marked with an x in the sam e row are not significantly different. ......... ................................. .................................. 28 Table 3-26. Results of Analysis of Variance of Impinged Alewife at Cook Nuclear Plant Unit

2. Data are Loglo (x+l) transform ed ............................................................................ 28 Table 3-27. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Alewife at Cook Nuclear Plant Unit 2. Months marked with an x in the same row are not significantly different ............................................................................... 29 Table 3-28. Comparison of fish impingement estimates between July 2005 through January 2006 and July 2006 through January 2007 at Cook Nuclear Plant Unit 2 .................. 29 20452 Cook 316b Baseline Final.doc 10/4/07 V NormandeauAssociates, Inc.

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316(b) PHASE II BASELINE FISH E & I STUDY Table 3-29. Mean Density (No./100 in 3 ) of Ichthyoplankton Collected at the Shoreline Station in the Vicinity of Cook Nuclear Plant July through November 2005, and April through N ovem ber 2006 ...................................................................................... 30 Table 3-30. Mean Density (No./100 M 3 ) of Ichthyoplankton Collected at the Surface, Mid-depth (11 ft) and Bottom (22 ft) of the Intake Station in the Vicinity of Cook Nuclear Plant June through November 2005, and April through November 2006 ........... 33 Table 3-31. Mean Density (No./100 mi3 ) of Ichthyoplankton Collected at the Surface to Mid-depth (0-20 ft) and Bottom (30-40 ft) of the Experimental Station in the Vicinity of Cook Nuclear Plant June through November 2005, and April through N ovember 2006 .................................................................................................................... 35 Table 3-32. Results of Analysis of Variance of Ichthyoptankton Densities in the Nearfield Area at Cook Nuclear Plant ............................................................................................ 36 Table 3-33. Mean Catch per Unit Effort (fish/hour) for Fish Captured in the Gill Net at the Intake Station (22 ft) and Experimental Station (40 ft) in the Vicinity of Cook Nuclear Plant, June through November 2005 and April through November 2006 .......... 38 Table 3-34. Mean Catch per Unit Effort (fish/trawl) for Fish Captured in the Otter Trawl at the Shoreline Station (5 ft) Intake Station (22 ft) and Experimental Station (40 ft) in the Vicinity of Cook Nuclear Plant, June through November 2005 and April through N ovember 2006 ................................................................................................ 40 Table 3-35. Results of Analysis of Variation of Trawl CPUE in the Nearfield Area of Cook Nuclear Plant. Data are Loglo (x+l) transform ed .............................................................. 44 Table 3-36. Results of Scheffe's Multiple Comparisons Test among Months for Otter Trawl CPUE (catch per trawl) in the Nearfield Area of Cook Nuclear Plant. Months marked with an x in the same row are not significantly different ................................ 45 Table 3-37. Results of Scheffe's Multiple Comparisons Test among Sampling Stations for Otter Trawl CPUE (catch per trawl) in the Nearfield Area of Cook Nuclear Plant.

Stations marked with an x in the same row are not significantly different ................. 45 Table 3-38. Mean Catch per Unit Effort (fish/haul) for Fish Captured in the Seine at the Shore Station in the Vicinity of Cook Nuclear Plant, June through November 2005 and April through November 2006 ...................................................................... 46 Table 4-1. Average number of fish eggs entrained at Cook Nuclear Plant between 1978 and 1982, and in the present study ....................................................................................... 49 Table 4-2. Annual Average number of fish larvae entrained at Cook Nuclear Plant between 1975 and 1982 and in the present study ........................................................................ 50 Table 4-3. Annual Average number of fish Impinged at Cook Nuclear Plant (Units 1 and 2) between 1975 and 1982 and in the present study ......................................................... 50 20452 Cook 316b Baseline Final.doc 10/4/07 vi NormandeauAssociates, Inc.

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316(b) PHASE II BASELINE FISH E & I STUDY Table 4-4. Mean Monthly Total Ichthyoplankton Density at Sampling Stations in the Vicinity of Cook Nuclear Plant June through November 2005 and April through N ovember 2006 ............................................................................................................ 52 20452 Cook 316b Baseline Final.doc 10/4/07 vii NormandeauAssociates, Inc.

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316(b) PHASE II BASELINE FISH E & I STUDY

1.0 INTRODUCTION

Impingement and entrainment studies were conducted at the Donald C. Cook Nuclear Plant (Cook Nuclear Plant) between June 2005 and January 2007 as part of the compliance effort associated with the Section 316(b), Phase II rule published by U.S. EPA in the Federal Register July 9, 2004. As part of the impingement mortality and entrainment studies, fish and ichthyoplankton sampling was conducted in Lake Michigan near the Cook Nuclear Plant from June 2005 through January 2007.

The Phase II rule established the procedures for complying with Section 316(b) at steam electric plants generating electricity for sale and distribution. Performance criteria were established in the rule for the protection of impinged and entrained aquatic and marine life. These criteria were measured against the calculation baseline, which was to be determined for each facility subject to the rule. The calculation baseline was defined in 40 CFR Section 125.93 as the estimated or actual impingement mortality and entrainment (IM&E) that would occur assuming that the cooling water system conforms with a standard configuration called the baseline case. in the rule, which including a bulkhead intake and there are no structural and operational controls that would reduce impingement mortality and entrainment, and the traveling water screens were 3 /8 tin, mesh. The rule intended the calculation baseline to be the estimated impingement mortality and entrainment rate for a facility that had no controls to reduce IM&E and used the most common location for intake structures on the water body.

Cook Nuclear Plant does not have a baseline case cooling water intake structure as defined in the rule. Details on the cooling water intake structure and cooling water flows are found in AEPSC (2005). Cooling water at Cook Nuclear Plant is withdrawn through three intake tunnels equipped with velocity caps located about 2,250 ft offshore. The location of these tunnels and velocity caps may serve to reduce IM&E from that which may occur at a baseline case intake structure. Ichthyoplankton and fish sampling in the vicinity of the intakes (nearfield area) was conducted to provide the data that may be used to estimate the reduction in IM&E due to the location and configuration of the intakes.

This report presents the results. of the impingement and entrainment monitoring program and nearfield sampling program. Impingement mortality and entrainment estimates are made for the study period assuming the impingement mortality and entrainment rates were proportional to intake flow and the plant used the design intake flow 100% of the year. Results from the nearfield sampling are also presented and discussed to characterize the fish community in the vicinity of the intakes.

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316(b) PHASE II BASELINE FISH E & I STUDY

.2.0 METHODS AND MATERIALS Detailed field and laboratory methods are presented in theproject Standard Operating Procedures (NAI 2007). The following is a summary of the methods used in the project.

2.1 IMPINGEMENT FIELD PROCEDURES Impingement samples were collected in the last week of June 2005, every other week from July 2005 through January 2006, and then twice per week February 2006 through January 2007. A total of 244 samples were scheduled for the period of June 2005 through January 2007, all of which were collected. The sample consisted of all screenwash material (debris and fish) trapped on the traveling screens during the 12 hour1.388889e-4 days <br />0.00333 hours <br />1.984127e-5 weeks <br />4.566e-6 months <br /> diel collection period. Screenwash material was washed from the screens and into metal mesh baskets (29.25 x 35 x 35 inches). The screenwash material from Units 1 and 2 were kept separate. Samples consisted of two 12-hour collection periods (0400-1600 and 1600-0400 hours). A typical sample day started with the. washing of the traveling screens at 0330 for 30 minutes to clear the traveling screens of material prior to the start of the 0400-1600 hrs sample.

The termination of the daytime sample (0400-1600 hrs) initiated the beginning of the night sample which ran from approximately 1600 until 0400 the next day. During events where the debris baskets filled before the preset sample termination, the baskets were emptied and all screenwash material sorted and fish enumerated. These fish were considered part of the overall diel sample and included in the final 12 hour1.388889e-4 days <br />0.00333 hours <br />1.984127e-5 weeks <br />4.566e-6 months <br /> sampling event.

Whenever possible, all fish were separated from the screenwash material and identified to species. Additionally, species were separated by length class (those estimated to be young of year (YOY) and those considered to be yearling or older). The first 50 of each species and~length class were measured (total length to the nearest mm), weighed (nearest g), and designated as live (moving, red gill filaments) or dead (decaying, light pink/white gill filaments). The remaining fish in a species and lifestage were counted and weighed as an aggregate. If an extremely large number of fish or lifestage were present, the number and weight of the remaining fish were estimated through gravimetric subsampling. In addition to the fish counts, the amount of debris collected in each sample was estimated and recorded.

Subsampling of the entire screenwash material occurred when there were large amounts of debris or fish. Subsampling consisted of taking a manageable section of the debris from the entire sample (rarely less than 1/10 of the total screenwash material). The fish from these subsamples were enumerated per unit volume and scaled up to the total volume of screenwash material impinged in the sample.

2.2 ENTRAINMENT METHODS Entrainment samples were collected from June-November 2005, and February 2006-January 2007. During 2005, entrainment samples were taken once per week from June 23, 2005 through August 31, 2005 and twice per month from September 1, 2005 through November 30, 2005. During 2006-2007, one monthly entrainment sample was collected during February, October, November, and December of 2006 and in January 2007. Sampling was conducted weekly between 20 March 2006 and 29.May 2006 as well as 4 September 2006 and 29 September 2006. Between 5 June and 28 August 2006, sampling was conducted twice weekly.

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316(b) PHASE II BASELINE FISH E & I STUDY Entrainment samples were collected using an electric trash pump with three inch intake and discharge openings. Water was drawn up through flexible three-in diameter hose suspended near the surface in the forebay and then pumped into a 0.300-mm mesh plankton net placed within a plastic tank. This was considered to be a well-mixed sample because water had already been withdrawn through the offshore intake and traveled through the 16-ft diameter intake tunnels located 2,250 ft offshore in 24 ft of water before reaching the forebay. Each sampling periods consisted of four samples of 6-hour duration during a 24-hour period (0300-0900, 0900-1500, 1500-2100, 2100-0300).

Water was pumped from a grate located near the center point of intake forebay from a depth approximately 8-10 ft below the water surface. Sample water was dischargediinto the entraiiment sampling tank at a rate of 80-110 gpm until the flow meter registered that over 27000 gallons, or 100 m3, of water had been sampled (approximately 5-6 hours). Previous entrainment sampling conducted in the Cook Nuclear Plantintake forebay indicated the water entering the intake bay was well mixed both horizontally and vertically and there was no potential for stratification of organisms (Bimber et.

al. 1984). Therefore the sample location ensured a uniform and representative sample. A total of 252 entrainment samples were scheduled and collected for the program.

The sampling pump was periodically calibrated to the known 110-gallon volume of the sample tank in order to-ensure that the amount of error between the calculated gallons/minute and that registered by the flow meter was less than 5 %.

At the termination of an entrainment sample, the pump was stopped and the net removed from the tank. The sample was washed down into the cod-end of the plankton net and then placed into a one quart sample jar. The net was then replaced and the pump restarted to begin the next sample. The jar was removed from the screenhouse and brought back to the on-site trailer where the sample was preserved in 5 % formalin.

2.2.1 Ichthyoplankton (Entrainment and Nearfield Sampling) Laboratory Processing Procedures Entrainment and nearfield ichthyoplankton samples were sorted to remove fish eggs and larvae. Samples with high abundances were sub-sampled with a Motoda plankton splitter to provide a minimum of 200 eggs and larvae combined. Specimens were identified (usually to species level) and enumerated by life stage (eggs, yolk-sac larvae, post yolk-sac larvae, young-of-the-year, and yearling or older). The descriptions of the larval stages are as follows:

Yolk-sac larva: the transition stage from hatching through the development of a complete, functional digestive system.

Post yolk-sac larva: the transitional stage forom the development of a complete functional digestive system to transformation to the juvenile form.

Young-of-the-year: the stage from complete transformation to Agel (ie. 12 months after hatching). A young-of-the-year has a full complement of fin rays identical to that of an adult.

Yearling or older: a fish at least one year old.

2.3 NEARFIELD SAMPLING The fisheries resources in the vicinity of the intakes were sampled to characterize the resources and to evaluate alternative intake locations (Figure 2-1). The ichthyoplankton community 20452 Cook 316b Baseline Final.doc 10/4/07 3 Normandeau Associates, Inc. "

316(b) PHASE II BASELINE FISH E & I STUDY Figure 2-1. Location of sampling stations in the nearfield area in the vicinity of Cook Nuclear Plant.

was sampled at six depth-station combinations using an ichthyoplankton net. The shoreline fish community was sampled with a beach seine. The demersal fish community was sampled with an otter trawl at three stations and the pelagic fish community was sampled with gill nets at two stations.

Water quality measurements were also taken at each station and depth when possible.

Dissolved oxygen (mg/1) and temperature (°C) were taken at each station while current (ft/sec) was measured at two gillnet stations.

Air temperature (fC), wind speed (mph), water clarity by Secchi disk depth (in), weather, and wave height (ft) and direction were also recorded for each sample when possible.

2.3.1 Ichthyoplankton Sampling Ichthyoplankton samples were collected at six stations. A day and night sample was collected once per month from June-November 2005 and April-November 2006 with a target volume of 50 M 3 . The night sampling on 2 June 2005 was not conducted due to weather resulting in 189 of the 196 possible samples collected.

A 0.5 m conical ichthyoplankton net and calibrated flow meter was used for the collection of samples at the following stations: shoreline station, between 1-6 ft deep (two samples), 22-ft intake 20452 Cook 316b Baseline Final.doc 10/4/07 4 Normandeau Associates, Inc.

316(b) PHASE II BASELINE FISH E & I STUDY station (samples taken at surface, 10 ft, and 22 ft), and the 40-ft experimental station (one sample combining surface, 10-ft, and 20-ft depth trawls, and one sample combining 30-ft and 40-ft depth trawls).

Surface tows were conducted by attaching a float to the top of the ichthyoplankton net ensuring the entire sample was from the surface. At depth tows were conducted by attaching a weight to the net and towing it by a line of predetermined length at a 450 angle to the water's surface to ensure that the sample was collected at the specified depths.

Samples collected were washed down into the cod end from the outside of the net and then transferred to sample jars. Sample were preserved in 5% formalin and labeled. Shoreline and intake station samples were individually preserved. At the experimental station, surface, 10-fl, and 20-ft tows were combined into one sample as will the 30-ft and 40-ft plankton tows.

2.3.2 Gill Net Sampling Experimental gill nets were used to sample pelagic fish communities at the 22-ft intake depth and the 40-ft experimental depth. Gill net samples were conducted during day and night once per month from June-November 2005 and April-November 2006. All 56 possible samples were collected during the study.

Sampling was conducted with 100-ft, four-panel experimental gill nets. Each 25-ft panel had a different mesh size (1/2-in, 1-in, 2-in, and 3-in bar mesh size). Gill nets were set at depth perpendicular to the eastern shoreline south of the no boating zone. Nets were fished for four hours and then retrieved. All fish were removed from the net and identified to species, measured (mm),

weighed (g) and released.

2.3.3 Otter Trawl Sampling An otter trawl was used to sample demersal fish communities at the shoreline station (6 ft),

intake depth (22 ft), and experimental depth (40 ft). Sampling was conducted June-November 2005 and April-November 2006. In 2005, no shoreline sampling occurred June-September (8 samples total) resulting in 76 samples collected of the 84 possible. The otter trawl was 18 ft long with 1/2-in bar mesh and a cod end with 1/8-in bar mesh.

Otter trawls were set from the boat at the specified depth and towed south (parallel to the shoreline) at a similar depth for approximately 400 m. The net was then retrieved and all fish were collected, identified to species, measured (mm), weighed (g) and released.

2.3.4 Seine Sampling Seine surveys were used to sample shoreline fish communities. Sampling occurred at two sites along the shoreline August-November 2005 and April-November 2006. No sampling occurred in June or July of 2005 resulting in 48 of 56 possible samples being collected.

A 100-ft bag seine (1/4-in bar mesh, 1/16-in bar mesh bag) was anchored to the shoreline while the other end was pulled out and then around back to the beach by the boat. The seine was then pulled in and the bag examined for fish. All fish were identified, measured (mm), weighed (g) and released.

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316(b) PHASE I/ BASELINE FISH E & l STUDY 2.4 ANALYTIC METHODS 2.4.1 Entrainment Analytic Methods Entrainment estimates were determined from sample counts and estimates of the actual volume of water pumped by the entrainment sampler during the sampling time frame. Specifically, sample volume was determined in the field based on flowmeter readings. Counts of entrained organisms determined in the lab were weighted by the sample split factor, and divided by the sample volume to calculate sample density for each diel sample:

Density= (count*split factor) /sample volume These estimates of density were then multiplied by the design cooling water intake flow for the diel period (specific to Unit 1 or 2) to estimate entrainment by the plant during the diel period.

Entrainment estimates were derived for each day during the study period. Non-sample day estimates were calculated from sample day densities and weighted in proportion to their temporal proximity to sample days. Assignment of month was based on the Sunday date for each week, regardless of when actual samples were taken. Daily estimates were summed to obtain monthly estimates, and monthly estimates summed to obtain annual estimates. When appropriate, calculations were performed by taxon and life stage.

2.4.2 Impingement Analytic Methods Densities of impinged organisms (No./unit of cooling water flow) were estimated for each diel sampling period based on the number of organisms impinged in the sample and the actual cooling water flow during the sample period. Impingement estimates for design cooling water flow were then estimated by multiplying these densities by the design cooling water flow for the diel period (specific to Unit 1 or 2).

Mean weekly diel estimates of impingement at Unit I or Unit 2 were determined from one or two samples per week, and then the weekly impingement estimate was determined by multiplying these mean estimates by 7. Monthly.estimates were calculated by summing the weekly estimates, and annual estimates were determined based on the sum of the monthly estimates. Assignment of weeks to months was based on the month in which the Sunday of the sample week fell.

Estimates for outage periods were calculated using actual sample data from the two sampled weeks preceding the outage, as well as the two sampled weeks following the outage. Weekly estimates during the outage periods were apportioned from the four actual samples according to the temporal distance the estimated sample was from the actual sample.

2.4.3 Nearfield Sampling Analytic Methods Catch per unit effort (CPUE) was calculated for each type of sampling effort in the nearfield 3

area. For ichthyoplankton sampling, CPUE was calculated as number of organisms per 100 m sampled. Gill net CPUE was expressed as number of fish per hour of soak time. Trawl and seine CPUE were expressed as number of fish per trawl or seine haul.

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316(b) PHASE II BASELINE FISH E & I STUDY 2.5 QUALITY ASSURANCE AND CONTROL (QA/QC) METHODS AND SAMPLE CHAIN OF CUSTODY Impingement and nearfield fisheries samples were analyzed at Normandeau's on-site field office at the Cook Nuclear Plant or in the. field and were subject to quality control inspections.

During the two year study at Cook Nuclear Plant AEP and Normandeau staff conducted two on-site quality assurance audits. The first audit was conducted by AEP in September 2005 and the second was conducted by Normandeau in January 2007. The AEP audit included impingement, entrainment and nearfield sampling. Otter trawl sampling at the shore station was added as a result of this audit.

No significant deficiencies were noted. The second quality assurance audit was conducted by Normandeau in January 2006 and evaluated impingement field collection and laboratory processing at the Cook Nuclear Plant facility. There were no deficiencies recorded in field collection process and laboratory processing of impingement samples in this audit.

After each impingement sample the identification of a subsample of the fish wvere verified by the field supervisor and the technician. Any species that were not readily identifiable were brought from the site of collection to the field office where they were identified using taxonomic keys. These fish were then sent to the biological lab in Bedford, NE where the identification was verified and they were added to the voucher collection.

Entrainment samples were shipped to Normandeau's Bedford New Hampshire Laboratory with a chain of custody documentation with the following information: Sample collection date, sample collection time, sample identification number and number of jars per sample. Upon receipt of the samples, a Bedford Laboratory representative verified that all jars of all samples were present and signed and dated the chain of custody document. In the laboratory samples were tracked during all phases of analysis by means of sample control logs.

2.5.1 Tasks Subject to Quality Control The following tasks were subjected to quality control checks consisting of reanalysis of randomly selected samples or measurements:

2.5.2 Inspection Plans Items are inspected using a quality control (QC) procedure derived from MIL-STD (military-standard) 1235B (single and multiple level continuous sampling procedures and tables for inspection by attributes) to achieve a 10 percent or better AOQL (Average Outgoing Quality Limit). The QC procedure used is the CSP-1 continuous sampling plan, which is conducted in two modes as follows:

Mode 1. One hundred percent of the samples were reinspected until "i" consecutive samples passed.

Mode 2. After "i" consecutive samples passed QC reinspeclion, a random numbers table was used to select the fraction "f' of the samples for reinspection. If any QC samples failed then that individual's quality control inspection returned to Mode 1.

For this application of CSP-1, i=8 and f=1/7, because the total number of samples analyzed' by an individual were less than 500. Items for reanalysis according to the plan were selected using a random number table. The original analyzer did not know whether a sample would be checked before the analysis of that sample had been completed. All quality control checks were performed "blindly" 20452 Cook 316b Baseline Final.doc 10/4/07 7 NormandeauAssociates, Inc.

316(b) PHASE I/BASELINE FISH E & I STUDY (i.e., the individual performing the QC inspection did not have knowledge of the original analyst's results).

The QC plan was applied on an individual processor basis, so that each person's work was subjected to the QC plan independently of others, starting at 100% inspection.

A resolution (third person) value may be determined for any sample found defective. All errors found during the QC check, whether the sample was found to be defective or not, were corrected on the data sheets. (A difference between original and QC counts that is within acceptable limits is not considered to be an error).

2.5.3 Acceptance/Rejection Criteria 2.5.3.1 Sorting A sample was considered defective if the sorter failed to remove 10 percent of the total organisms in the sample (or subsample). Percent error was calculated as follows (where "QC count" denotes the number missed by the sorter):

2.5.3.2 Identification A sample was considered defective if an error of 10 percent or more is made in identifying, assigning a life stage, or counting any species. In determining whether a sample is defective, analyzer and QC results were compared within each taxon/life stage combination.

For each taxon (or for a life stage within a taxon) the percent error was calculated as follows (except where the QC count is <20, the percent error is considered to be zero if analyzer and QC counts differ by no more than two organisms):

% error = 100% x I analyzer count - QC count I/QC count A sample with a percent error of greater than or equal to 10% for any life stage for any taxon was considered defective.

For each defective sample, a resolution was determined in which a third person reanalyzed

'the sample (resolution value). The error for each species and life stage was then be calculated using the resolution counts as the divisor. This was done for both identification and QC counts:

% error = 100% x Iidentifier count - resolution count /resolution count

% error = 100% x I QC count - resolution count I/resolution count If the resolution vs. identifier error was <10 percent, the sample passed. If they were not, the sample failed and identifier counts were replaced by QC counts for all cases, provided the QC vs.

resolution error was <10 percent.' If the resolution vs. identifier and the resolution vs. QC errors were both 10 percent or more, the sample was thoroughly reviewed by all three people and the identifier's sample processing did not continue until agreement was reached on the identification of the sample.

20452 Cook 316b Baseline Final.doc 10/4/07 8 NormandeauAssociates, Inc.

1(,o .

316(b) PHASE II BASELINE FISH E & I STUDY 2.5.4 Quality Control Records Quality control logs were carefully maintained, documenting the samples analyzed, the samples selected for reanalysis according to the QC plan, the results of the QC analysis, and any corrective action performed.

2.5.5 Reference Collection Each taxon and life stage identified in the Cook Nuclear Plant entrainment program was represented in the general ichthyoplankton reference collection at Normandeau's biology laboratory.

In addition, type specimens were retained for all taxa identified in the impingement and nearfield fisheries program.

2.5.6 Data Processing 2.5.6.1 Data Entry Verification and Data Sheet Chain of Custody A submittal form with each batch of data sheets was submitted to the Technical Data Processing (TDP) department for data entry. Information on the submittal form included names of sender and recipient, date sent, and dates of impingement collections included in the batch. All data entries were keypunched twice, and discrepancies between the two versions were resolved as they were flagged by the data verification program. - -

2.5.6.2 Systematic Error Checks Keyed data were subjected to a series of systematic error checking programs developed specifically for the Cook Nuclear plant Project. These checks consisted of univariate, bivariate, and multivariate checks. Univariate range checks identified records for which one or more variables had values outside their valid or expected ranges. Bivariate and multivariate checks compared values of related variables. Additional checks scanned the data for duplicate or missing observations. All records flagged by these programs were resolved, and corrections to both the data files and the data sheets were made as necessary.

2.5.6.4 Quality Control of Data Files After the systematic error checking process data files underwent a QC inspection to assure a 1% AOQL (Average Outgoing Quality Limit) according to a lot sampling plan (American Society for Quality Control 1993). This procedure insured that >99% of the observations in a data file agreed with the original data sheets.

20452 Cook 316b Baseline Final.doc 10/4/07 9 NormandeauAssociates, Inc.

1-1

316(b) PHASE I BASELINE FISH E & I-STUDY 3.0 RESULTS Impingement and entrainment sampling took place at Cook Nuclear Plant for 20 consecutive months from June 2005 through January 2007. This results section will focus on the 12 month period from February 2006 through January 2007 (primary study period) to provide annual estimates of the number of organisms entrained and impinged at the plant. The remaining eight months of June 2005 through January 2006 will be compared with the corresponding months of June through January 2006 to provide an estimate of the variability in entrainment and impingement between years. Appendix Tables A through G present monthly estimates of entrainment of each lifestage for each month of the study.

Nearfield sampling took place from June through November 2005 and April through November 2006. The entire data set will be used to characterize the ichthyoplankton and fish community in the vicinity of Cook Nuclear Plant.

3.1 ENTRAINMENT An estimated 105.72 million fish eggs, larvae and older fish were entrained at Cook Nuclear Plant from February 2006 through January 2007 (Table 3-1;Appendix Table A). Larvae were the predominant lifestage entrained (Figure 3-1). The greatest amount of entrainment occurred in July (41.90 million) and June 2006 (36.47 million), accounting for 74% of the annual total. Cyprinids (10.51 million in June) and round goby (27.68 million in July) were the most common taxa entrained in June and July 2006. For the entire 12-month period, round goby, cyprinids, and alewife were the most common taxa entrained, accounting for 82% of the annul total.

An estimated 16.86 million fish eggs were entrained during the 12-month primary study period (Table 3-2; Figure 3-2; Appendix Table B). Most (87%) of the egg entrainment occurred in June 2006 (14.74 million) and this was primarily alewife eggs (7.01 million). Alewife (7.08 million) cyprinid (4.42 million) and unidentified eggs (3.78 million) were the most common egg taxa entrained during the primary study period, accounting for 91% of the annual total.

An estimated 83.82 million fish larvae were entrained during the primary study period (Table 3-3; Figure 3-3; Appendix Tables C, D, E,). Most of this entrainment occurred in June (21.59 million) and July (41.52 million) 2006 accounting for 75% of the annual total. In June and July, round goby (9.73 million in June; July: 27.68 million in July) and cyprinids (7.63 million in June; 7.39 million in July) were the dominant fish larvae entrained. For the 12-month primary study period, round goby (44.04 million) and cyprinids (16.87 million) were the most common larval taxa entrained, accounting for 87% of the annual total.

An estimated 5.04 million YOY and older fish were entrained during the primary study period (Table 3-4; Figure 3-4; Appendix Tables F and G). Highest entrainment of YOY and older fish occurred in August when 1.09 million fish were entrained. The majority (54%) of the August entrainment were alewife (0.59 million). For the 12-month primary study period, yellow perch (1.58 million), spottail shiner (1.13 million), and round goby (1.08 million) were the most common fish entrained.

Entrainment data collected from July 2005 through January 2006 and July 2006 through January 2007 provide an opportunity to assess variability in entrainment estimates among comparable 20452 Cook 316b Baseline Final.doc 10/4/07 10 Normandeau Associates, Inc.

316(b) PHASE II BASELINE FISH E & I STUDY Table 3-1. Estimated number (in millions) of fish eggs, larvae, and young-of-year and older fish Entrained by Month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007.

Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan-06 06 06 06 06 06 06 06 06 06 06 07 Total Alewife 0.00 0.00 0.00 0.00 10.57 6.23 1.48 1.91 0.00 0.00 0.00 0.00 20.19 Common carp 0.00 0.00 0.00 0.00 1.58 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.58 Rainbow smelt 0.51 0.00 0.00 0.00 0.00 0.09 3.93 5.24 0.00 0.00 0.00 0.00 9.77 Round goby 0.04 0.41 0.49 0.64 9.73 27.68 4.23 1.90 0.00 0.00 0.00 0.00 45.11 Round 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 whitefish Slimy sculpin 0.00 0.00 0.00 0.00 0.27 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.27 Spottail shiner 0.00 0.00 0.12 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.29 0.58 1.13 Unidentified 0.00 0.08 0.19 0.24 3.50 0.19 0.00 0.00 0.00 0.00 0.00 0.00 4.21 Unidentified 0.00 0.00 0.00 2.96 10.51 7.61 0.20 0.00 0.00 0.00 0.00 0.00 21.29 cyprinidae White sucker 0.00 0.00 0.00 0.13 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 Yellow perch 0.04 0.37 0.00 0.00 0.30 0.09 0.36 0.11 0.00 0.15 0.46 0.00 1.88 All species 0.59 0.86 0.95 4.11 36.47 41.90 10.20 9.16 0.00 0.15 0.75 0.58 105.72 45.00 40.00 3500 E

0 OY-O-Yand Older

• Larvae I--

W o Eggs E

0u 0.00 Feb-06 Mar-06 Apr-06 May-06 Jun-06 Jul-06 Aug-06 Sep-06 Oct-06 Nov-06 Dec-06 Jan-07 Month Figure 3-1. Estimated total entrainment (in millions) of fish eggs, larvae, and young-of-the-year and older fish at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007.

20452 Cook 316b Baseline Final.doc 10/4/07 I1I NormandeauAssociates, Inc.

316(b) PHASE II BASELINE FISH E & I STUDY Table 3-2. Estimated number (in millions) of Fish Eggs Entrained by Month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007.

Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan-06 06 06 06 06 06 06 06 06 06 06 07 Total Alewife 0.00 0.00 0.00 0.00 7.01 0.07 0.00 0.00 0.00 0.00 0.00 0.00 7.08 Common carp 0.00 0.00 0.00 0.00 1.58 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.58 Rainbow smelt 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Round whitefish 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Slimy sculpin 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spottail shiner 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Unidentified 0.00 0.08 0.19 0.24 3.27 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3.78 Unidentified 0.00 0.00 0.00 1.32 2.88 0.22 0.00 0.00 0.00 0.00 0.00 0.00 4.42 cyprinidae White sucker 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Yellow perch 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 All species 0.00 0.08 0.19 1.56 14.74 0.29 0.00 0.00 0.00 0.00 0.00 0.00 16.86 16 12 0

10 E

C

" 6 4

4 Feb-06 Mar-06 Apr-06 May-06 Jun-06 Jul-06 Aug-06 Sep-06 Oct-06 Nov-06 Dec-06 Jan-07 Month Figure 3-2. Estimated species composition of fish eggs entrained (in millions) by month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007.

20452 Cook 316b Baseline Final.doc 10/4/07 12 NormandeauAssociates, Inc.

316(b) PHASE I BASELINE FISHE & I STUDY Table 3-3. Estimated number (in millions) of Fish Larvae Entrained by Month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007.

Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan-06 06 06 06 06 06 06 06 06 06 06 07 Total Alewife 0.00 0.00 0.00 0.00 3.42 6.16 0.89 1.91 0.00 0.00 0.00 0.00 12.38 Common carp 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 0.00 0.09 3.93 5.24 0.00 0.00 0.00 0.00 9.26 Round goby 0.00 0.00 0.00 0.64 9.73 27.68 4.09 1.90 0.00 0.00 0.00 0.00 44.04 Round whitefish 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 Slimy sculpin 0.00 0.00 0.00 0.00 0.27 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.27 Spottail shiner 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Unidentified 0.00 0.00 0.00 0.00 0.24 0.19 0.00 0.00 0.00 0.00 0.00 0.00 0.43 Unidentified 0.00 0.00 0.00 1.64 7.63 7.39 0.20 0.00 0.00 0.00 0.00 0.00 16.87 cyprinidae White sucker 0.00 0.00 0.00 0.13 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 Yellow perch 0.00 0.00 0.00 0.00 0.30 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.30 All species 0.00 0.00 0.14 2.41 21.59 41.52 9.11 9.05 0.00 0.00 0.00 0.00 83.82 45 40 35 30 E 25

._5 20

• 15 E

Feb-06 Mar-06 Apr-06 May-06 Jun-06 Jul-06 Aug-06 Sep-06 Oct-06 Nov-06 Dec-06 Jan-07 Month Figure 3-3. Estimated species composition of fish larvae entrained (in millions) by month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007.

20452 Cook 316b Baseline Final.doc 10/4/07 13 Normandeau Associates, Inc.

316(b) PHASE IIBASELINE FISH E &J STUDY Table 3-4. Estimated number (in millions) of Young-of-the-Year and Older Fish Entrained by Month at Cook Nuclear Power Plan Assuming Design Cooling Water Flow, February 2006 through January 2007.

Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Total 06 06 06 06 06 06 06 06 06 06 06 07 Alewife 0.00 0.00 0.00 0.00 0.14 0.00 0.59 0.00 0.00 0.00 0.00 0.00 0.74 Common carp 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rainbow smelt 0.51 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.51 Round goby 0.04 0.41 0.49 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 1.08 Round whitefish 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Slimy sculpin 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spottail shiner 0.00 0.00 0.12 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.29 0.58 1.13 Unidentified 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Unidentified 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 cyprinidae II White sucker 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Yellow perch 0.04 0.37 0.00 0.00 0.00 0.09 0.36 0.11 0.00 0.15 0.46 0.00 1.58 All species 0.59 0.79 0.61 0.14 0.14 0.09 1.09 0.11 0.00 0.15 0.75 0.58 5.04 E

4-E 0.8 V

C 0

• 0 0.6 0.6

. 0.4 0

0(

E 0.2 i

Feb-06 Mar-06 Apr-06 May-06 Jun-06 Jul-06 Aug-06 Sep-06 Oct-06 Nov-06 Dec-06 Jan-07 Month Figure 3-4. Estimated species composition of young-of-the-year and older fish larvae entrained (in millions) by month at Cook Nuclear Plant Assuming Design Cooling Water Flow, February 2006 through January 2007.

20452 Cook 316b Baseline Final.doc 10/4/07 14 NormandeauAssociates, Inc.

316(b) PHASE /I BASELINE FISH E & I STUDY Table 3-5. Comparison of fish entrainment estimates (in millions) between July 2005 through January 2006 and July 2006 through January 2007 at Cook Nuclear Plant.

Lifestage j Year [ Jul. Aug. [ Sep. [ Oct. [ Nov. [ Dec. [ Jan. Total Total 2006-2007 41.90 10.20 9.16 0.00 0.15 0.75 0.58 62.74 2005-2006 106.32 27.20 13.11 4.21 1.92 2.91 0.81 156.48

% Difference 154 167 43 1180 288 40 149 Eggs 2006-2007 0.29 00 0 0 0 0 0 0.29 2005-2006 43.22 0 0 0 0 0 0 43.22

% Difference 14803 0 0 0 0 0 0 , 14803 Larvae 2006-2007 41.52 9.11 9.05 0 0 0 0 59.68 2005-2006 62.67 26.42 12.07 3.95 0 .0 0 105.11

% Difference 51 190 33 0 0 0 76 YOY and Older 2006-2007 0.09 1.09 0.11 0 0.15 0.75 0.58 2.77 2005-2006 0.43. 0.79 1.04 0.26 1.92 2.91 0.81 8.16

% Difference 378 -28 845 1180 288 40 195 There were substantial differences in entrainment estimates among diel periods (Table 3-6).

Entrainment for all life stages combined was highest in the 2100-0300 diel period, followed by the 0300-0900 diel period. Entrainment of eggs was relatively similar among diel periods, but larval entrainment was much higher in 2100-0300 period compared to the other periods. Entrainment of post yolk-sac round goby was much higher in the 2100-0300 diel period (36.84 million) compared to the other three diel periods (0.07- 6.97 million; Appendix Table E) and made the largest contribution to overall higher entrainment in this diel period.

Analysis of variance (ANOVA) was used to quantify the influence of month and diel period on entrainment estimates of each lifestage. Eggs were only entrained in March through July and there were significant differences in entrainment estimates among months (Table 3-7; Figure 3-2). -Egg entrainment in June 2006 was significantly greater than all other months and egg entrainment in May 2006 was significantly greater than February 2006, August through December 2006, and January 2007 (Table 3-8). High estimates of alewife egg entrainment in May and June 2006 made these months significantly different from the majority of the other months.

Monthly entrainment estimates for larvae did not show a consistent pattern among diel periods and months, resulting in a significant interaction term of these two main effects. In general, larval entrainment was highest in the 2100-0300 hrs and 0300-0900 hrs diel periods (Table 3-5) and in June and July 2006 (Table 3-3). However, the significant interaction term between these main effects in the ANOVA indicates that the relationship varied among diel periods and months.

Entrainment estimates inma few month-diel combinations such as July 2006, 2100-0300 hours, June 2006, 2100-0300 hours, and July 2006, 0300-0900 hours were significantly higher than the other 45 month-diel combinations. Post yolk-sac round goby larvae were dominant in the entrainment estimates for these periods along with post yolk-sac alewife larvae in the July 2006 0300-0900 estimate (Appendix Table E).

Entrainment patterns of YOY and older fish also were also not consistent among months and diel period combinations as indicated by the significant interaction term. Entrainment of these lifestages was generally highest in August and lower in the 0900-1500 hours diel periods. However, entrainment of these lifestages was significantly higher in March 2006 1500-2 100 hours0.00116 days <br />0.0278 hours <br />1.653439e-4 weeks <br />3.805e-5 months <br />, August 2006 20452 Cook 316b Baseline Final.doc 10/4/07 15" NormandeauAssociates, Inc.

316(b) PHASE I BASELINE FISH E & I STUDY 0300-0900 hours, and January 2007 0300-0900 hours month-diel combinations compared to most of the other 45 month-diel combinations. Yearling and older yellow perch and round goby were Table 3-61 Entrainment estimates (in millions) for each lifestage among the four diel periods sampled at the Cook Nuclear Plant.

Diel Period IEggs[ Larvae YOY and Older All Lifestages Alewife 0300-0900 1.24 5.5 0.45 7.33 0900-1500 1.87 1.48 0.22 3.57 1500-2100 0.29 2.21 0 2.51 2100-0300 3.68 3.04 0.07 6.79 Rainbow smelt 0300-0900 0 4.2 0.5.1 4.7 0900-1500 0 0.14 0 0.14 1500-2100 0 1 0 1 2100-0300 0 3.92 0 3.92 Spottail shiner 0300-0900 0 0 0 0 0900-1500 0 0 0 0 1500-2100 0 0 0.26 0.26 2100-0300 0 0 0.87 0.87 Yellow perch 0300-0900 0 0 0.28 0.28 0900-1500 0 0.3 0 0.3 1500-2100 0 0 1.09 1.09 2100-0300 0 0 0.21 0.21 All species combined 0300-0900 3.92 23.48 1.38 32.14 0900-1500 2.58 2.44 0.22 5.46 1500-2100 5.56 3.36 1.77 11.11 2100-0300 4.79 48.82 1.68 57.01 Table 3-7. Results of Analysis of Variance of Entrained Ichthyoplankton Lifestages at Cook Nuclear Plant. Data are Loglo (x+1) transformed.

Lifestage Source of Variation I df MS Pr>F Eggs Month(Year) 11 0.083 <0.001 Diel 3 0.002 0.517 Month(Year) X Diel 33 0.001 0.994 Error 160 0.003 Larvae Month(Year) 11 0.304 <0.001 Diel 3 0.306 <0.001 Month(Year) X Diel 33 0.059 <0.001 Error 160 0.007 YOY and Older Month(Year) 11 0.001 <0.001 Diel 3 0.002 <0.001 Month(Year) X Diel 33 0.001 <0.001 Error 160 <0.001 20452 Cook 316b Baseline Final.doc 10/4/07 . 16 Normandea~uAssociates,.Inc.

316(b) PHASE II BASELINE FISH E & I STUDY Table 3-8. Results of Least Squares Mean Multiple Comparisons Tests among Months for Monthly Egg Entrainment Estimates at Cook Nuclear Plant. Months marked with an x in-the same row are not significantly different.

Month and Year Sep- Oct- Nov- Dec- Aug- Feb- Jan- Mar- Apr- Jul- May- Jun-06 06 06 06 06 06 07 06 06 06 06 [ 06 x ' xxxx x x dominant in March 2006, 1500-2100 hours, YOY alewife were dominant in August 2006, 0300-0900 hours, and YOY spottail shiner were dominant in January, 2007 2100-0300 hours (Appendix Tables F and G).

3.2 IMPINGEMENT Impingement estimates are presented and discussed for Units 1 and 2 of Cook Nuclear Plant.

3.2.1 'Unit 1 An estimated 561,211 fish were impinged at Unit 1 between February 2006 and January 2007, with the greatest amount of impingement occurring in February 2006 (237,285 fish) (Figure 3-5; Table 3-9). The majority of the fish impinged in the 12 month period were yellow perch (442,580; 79%), alewife (41,812; 7%) and spottail shiner (38,350; 7%). Impingement of yellow perch occurred in every month, but was highest in February, December, and March. Alewife were impinged every month except October and counts were highest in June. Spottail shiner were impinged every month and impingement was highest in December. These three fishes accounted for 93% of the fish impinged at Unit 1.

There were significant differences in total impingement estimates among months, but no significant differences among diel periods (Table 3-10). Impingement was lower in the summer and early fall months compared to most other months (Table 3-11; Figure 3-5).

Yellow perch were the most common fish impinged at Unit 1 and there were significant differences among months, but not diel periods (Tables 3-12, 3-13). Impingement of yellow perch was highest in February and December 2006 and lowest in the summer and fall (Figure 3-5). Fish from the 2005 year class were dominant in February 2006 and fish from the 2006 year class were dominant in December 2006.

There were significant differences in alewife impingement among months at Unit 1 but not between diel periods (Tables 3-14, 3-15). In general, impingement of alewife was lowest in February through March and in September through October. Impingement of alewife was highest in June and November 2006 (Figure 3-4) and these were predominantly from the 2005 year class in June and the 2006 year class in November.

Impingement of spottail shiner at Unit 1 was significantly difference among months but not between diel periods (Tables 3-16, 3-17). Impingement was lowest in the summer months and highest in December, February and March. These were predominantly yearling and older fish (Figure 3-5).

20452 Cook 316b Baseline Final.doc"lOI4107 17 2 Normandeau Associates, Inc.

K3 C) Table 3-9. Estimated Number of Fish Impinged, at Cook Nuclear Plant Unit 1, Assuming Design Cooling Water Flow, February 2006 through January 2007.

0 C.) 2006 2007

3 Species February IMarch] April May June J July IAugust September October November December January Total 0n a)

Alewife 193 29 63 1,015 34,170 592 698 18 0 3,351 1,492 192 41,812 Bloater 14 44 60 0 0 0 0 15 0 14 219 11 375 CD Bluegill 7 218 25 0 0 0 0 1 10 0 67 21 347 .1h

-E CD Bluntnose minnow 0 0 0 0 4 0 0 0 0 0 0 0 4 Cn, Brook silverside 0 14 0 0 0 0 0 0 0 0 0 0 14 Brown bullhead 0 11 4 0 0 0 0 0 0 0 0 0 15 Brown trout 0 20 4 4 3 93 0 0 0 0 0 0 124 rrl Burbot 21 98 7 18 7 0 0 0 0 17 22 31 222 In Centralmudminnow 0 48 4 0 0 0 0 0 0 0 .0 0 52 0.

Channel catfish 469 315 64 .14 11 0 18 1 10 15 31 101 1,048 Chestnut lamprey 0 0 0 0 0 0 0 0 0 0 0 0 0 C,,

Chinook 0 98 21 0 0 0 0 0 0 0 0 0 119 -*1 Z

C) Coho 0 20 14 0 0 0 0 0 0 0 0 0 34 Common carp 0 4 0 0 3 0 0 0 0 0 14 0 21 0- Deepwater sculpin 0 14 4 0 0 0 0 0 0 20 0 0 38 Eastern banded killifish 0 34 0 0 0 0 0 0 0 0 0 0 34 Flathead catfish 0 0 0 0 0 0 0 0 0 0 0 0 0 Freshwater drum 0 0 0 11 0 0 4 6 4 0 8 0 33 Gizzard shad 381 1,085 21 0 0 0 0 5 68 481 5,056 1,130 8,227 Golden redhorse 0 0 0 11 0 0 0 0 0 0 14 15 40 Golden shiner 0 0 0 0 0 .0 - 0 0 0 0 0 0 0 Greater redhorse 0 0 0 0 .0 0 0 0 0 0 0 7 7 Lake chub 0 0 0 0 0 0 0 0 0 0 0 0 0 Lake sturgeon 0 0 0 0 0 0 0 0 0 0 14 0. .14 Lake trout 0 4 0 4 14 0 0 0. 0 3 29 0 53 Lake whitefish 713 137 56 7 0 14 0 0 0 28 2,715 765 4,436 Largemouth bass 0 0 0 0 0 0 0 0 0 0 0 0 0 Longnose dace 0 0 0 0 0 0 0 0 0 0 0 0 0 Longnose sucker .18 216 74 35 21 7 4 0 0 0 56 61 492 Mottled sculpin 0 "0 7 0 0 0 0 0 0 3 0 0 11 Ninespine stickleback 14 0 42 91 10 0 0 0 0 0 0 0 158 Northern pike 0 20 0 0 0 0 0 0 0 0 0 0 20 I (continued)

N) 0 Table 3-9. (Continued) 0 2006 2007 C.o Species February jMarch [ April [ May J June July August September October November December January Total CD Pumpkinseed 4 15 0 0 0 0 0 0 0 0 0 0 18 a, Rainbow smelt 1,450 827 210 301 11 21 4 0*

0 0 7 49 415 704 3,997 0 CO)

Rock bass 0 13 0 0 0 0 0 0 0 0 0 7 20 Round goby 983 928 1,282 6,648 1,491 956 517 205 64 788 1,559 508 15,929 p a,

Sea lamprey 200 78 25 4 0 0 0 0 0 0 0 70 ,376 Shorthead redhorse 0 4 0 0 0 0 0 0 0 0 0 0 4 rrI Silver redhorse 0 0 0 0 0 0 0 0 0 0 0 0 0 Slimy sculpin 119 168 229 67 88 14 0 0 0 4 0 14 702 co Smallmouth bass 4 . 0 0 0 0 0 0 03 0 0 0 0 4 Spottail shiner 7,425 3,442 2,686 655 129 98 165 . 73 15 652 20,675 2,336 38,350 Zo Steelhead 7 10 4 0 0 0 0 0 0 0 0 0 21 C,,

Threespine stickleback 475 150 56 60 38 0 , 0 0 0 0 26 60 866 90 Trout-perch 0 49 0 17 10 0. 0 0, 0 14 150 24 265 Unidentified 0 0 0 0 0 0 0 0 0 0 0 0 0 Walleye 0 0 0 0 0 0 0 .0 0 0 0 0 0 White perch 35 28 0 0 4 0 0 0 0 0 14 28 108 White sucker 4 71 4 28 32 3 0 4 0 0 28 48 221 Yellowperch 224,751 62,419 26,670 11,821 2,410 675 4,912 311 46 16,586 71,524 20,454 442,580 Total 237,285 70,631 31,635 20,808 38,455 2,473 6,321 638 225 22,026 104,128. 26,586 561,211 0

C)

M 0

0

316(b) PHASE II BASELINE FISH E & I STUDY 250000 200000 MOther SSpottail Shiner 150000 E

V "L

100000 E

W 50000 Feb-06 Mar-06 Apr-06 May-06 Jun-06 Jul-06 Aug-06 Sep-06 Oct-06 Nov-06 Dec-06 Jan-07 Month Figure 3-5. Estimated total number of fish impinged and species composition by month at Unit 1 of Cook Nuclear Power Place February 2006 through January 2007.

Table 3-10. Results of Analysis of Variance of Impinged Fish at Cook Nuclear Plant Unit 1.

Data are Log1o (x+l) transformed.

Species Source of Variation Df MS Pr>F All Species Month (Year) 11 6.08 <0.001 Diel 1 0.13 0.529 Month (Year) X Diel 11 0.03 1.000 Error 84 0.34 Table 3-11. Results of Scheffe's Multiple Comparisons Test among Months for Total Impingement Estimates at Cook Nuclear Plant Unit 1. Months marked with an 3 in the same row are not significantly different.

Month and Year Feb-06 Dec- Mar- Jan- May- Apr- Jun- Nov- Aug- Jul-06 Sep- Oct-06 06 07 06 06 06 06 06 06 06 x x x x x x x x x x x x x x x x x x x x x

____ I I x x x 20452 Cook 316b Baseline Final.doc 10/4/07 20 NormandeauAssociates, Inc.

316(b) PHASE II BASELINE FISH E & I STUDY Table 3-12. Results of Analysis of Variance of Yellow Perch Impingement at Cook Nuclear Plant Unit 1. Data are Logio (x+l) transformed.

Species Source of Variation df MS Pr>F Yellow Perch Month(Year) 11 9.47 <0.001 Diel I 0.30 0.452 Month(Year) X Diel 11' 0.07 0.100 Error 84 0.53 Table 3-13. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Yellow Perch at Cook Nuclear Plant Unit 1. Months marked with an x in the same row are not significantly different.

__ Month and Year Feb-06 Dec- Mar- Jan- Apr- May- Nov- Aug- Jun- Jul-06 Sep- Oct-06 06 07 06 06 06 06 06 06 06 X XX X X. X X X x x x x x x x x __

_x x x x x x

__ _X X X X X X - X X X X Table 3-14. Results of Analysis of Variance of Alewife Impingement at Cook Nuclear Plant Unit 1. Data are Logio (x+l) transformed.

Species I Source of Variation df MS Pr>F Alewife Month(Year) 11 5.71 <0.00i Diel 1 0.01 0.919 Month(Year) X Diel 11 0.05 1.000 Error 84 0.78 Table 3-15. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Alewife at Cook Nuclear Plant Unit 1. Months marked with an x in the same row are not significantly different.

Month and Year Jun-06 Nov- Dec- May- Aug- Jul-06 Jan- Feb- , Apr- Mar- Sep- Oct-06 06 06 06 106 06 06 06 06 06 lx  :  : X X' XX X X X x x x x x I x x x x x Table 3-16. Results of Analysis of Variance of Spottail Shiner Impingement at Cook Nuclear Plant Unit 1. Data are Logio (x+l) transformed.

Species I Source of Variation df MS I Pr>F Spottail Shiner Month (Year) 11 7.13 <0.001 Diel 1 0.06 0.746 Month (Year) X Diel 11 0.27 0.800 Error 84 0.44 20452 Cook 316b Baseline Final.doc 10/4/07 21 NormandeauAssociates, Inc.

316(b) PHASE I/ BASELINE FISH E & I STUDY Table 3-17. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Spottail Shiner at Cook Nuclear Plant Unit 1. Months marked with an x in the same row are not significantly different.

Month and Year Dec-06 Mar- Feb-06 Jan- Apr- Nov- May- Jun- Aug- Jul-06 Sep- Oct-06 07 06 106 06 1 06 06 06 06 X X X X X X X X X X X X X X X X X X X X X X X. X X X X X X X X X Impingement data collected from July 2005 through January 2006 and July 2006 through January,2007 provide an opportunity to assess variability in impingement estimates among comparable months (Appendix Table H). Total impingement was about 63% greater for the same months in 2005-2006 compared to 2006-2007 (Table 3-18). The greatest variation between the two years occurred in September and October. Greater numbers of yellow perch, gizzard shad, and spottail shiner were impinged in September and October of 2005 compared to 2006.

Impingement of fish biomass showed a similar seasonal pattern to numerical impingement, but the relative contribution among species was slightly different (Figure 3-6). An estimated 5,366 kg of fish were impinged at Unit I between February 2006 and January 2007 with the greatest amount of biomass impingement (1,395 kg) occurring in December 2006 (Appendix Table J). Similar to fish impingement, yellow perch were the largest component (2,452 kg) of the total biomass impingement making up 46% of the total. In contrast to fish impingement, lake whitefish.(675 kg; 13%) and spottail shiner (337 kg; 6%) ranked second and third in impingement biomass (Appendix Table 1).

Biomass impingement of yellow perch was greatest in February of 2006 when 822 kg Were impinged.

Lake whitefish and spottail shiner biomass impingement was greatest in December 2006 when 361 kg and 190 kg were impinged.

3.2.2 Unit 2 An estimated 824,812 fish were impinged at Unit 2 between February 2006 and January 2007 (Figure 3-7; Table 3-19). The majority of these fish were yellow perch (673,720; 82%), spottail shiner (48,970; 6%) and alewife (40,893; 5%). Impingement of yellow perch occurred every month, but was highest in February (352,834). Similarly, impingement of spottail shiner and alewife occurred in every month. Spottail shiner impingement was greatest in December (27,121) and impingement of alewife was greatest in June (30,897). These three fish composed 93% of the total impingement.

There were significant differences in total impingement estimates among months, but no significant differences among diel periods (Table 3-20). Impingement was lower in the summer and early' fall months compared to most other months (Table 3-21, Figure 3-7). Impingement was highest in February, December .andMarch primarily due to impingement of yellow perch and spottail shiner.

20452 Cook 316b Baseline Final.doc 10/4/07 22 NormandeauAssociates, Inc.

316(b) PHASE II BASELINE FISH E & I STUDY Table 3-18. Comparison of fish impingement estimates between July 2005 through January 2006 and July 2006 through January 2007 at Cook Nuclear Plant Unit 1.

Year Jul Aug I Sep L Oct Nov I Dec Jan I Total 2006-2007 2005-2006

% Difference 1600 t 2,473 853

-65 6,321 7,706 22 638 62,923 9,762 t 225 8,034 3,470 22,026 122,765 457 4' 104,128 20,461

-80 26,586 42,656 60 162397 265,398 63 x

C C

E a

C 0.

2 a

'a (U

2 0

0 0

0

'U

'a uJ Feb-06 Mar-06 Apr-06 May-06 Jun-06 Jul-06 Aug-06 Sep-06 Oct-06 Nov-06 Dec-06 Jan-07 Month Figure 3-6. Estimated biomass (kg) of fish impinged and biomass composition by month at Unit I of Cook Nuclear Plant February 2006 through January 2007.

20452 Cook 316b Baseline Final.doc 10/4/07 23 NormandeauAssociates, Inc.

316(b) PHASE II BASELINE FISH E & I STUDY 400000 350000 300000

• 250000 E

• 200000 E

E 150000 tu 100000 50000 0

Feb-06 Mar-06 Apr-06 May-06 Jun-06 Jul-06 Aug-06 Sep-06 Oct-06 Nov-06 Dec-06 Jan-07 Month Figure 3-7. Estimated total number of fish impinged and species composition by month at Unit 2 of Cook Nuclear Plant February 2006 through January 2007.

20452 Cook 316b Baseline Final.doc 10/4/07 24 NormandeauAssociates, Inc.

Table 3-19. Estimated Number of Fish Impinged, at Cook Nuclear Plant Unit 2, Assuming Design Cooling Water Flow, February 2006 through January 2007.

2006 2007 Species February J March J April] May [ June J July] August]. September October [November [December January TOTAL Alewife 152 31 .20 1,286 30,897 793 538 140 569 5,281 1,040 146 40,893 to Bloater 32 13 8 0 0 0 4 25 270 84 194 11 640 Cn Bluegill 32 274 183 18 0 0 0 0 4 60 67 24 . 661 Bluntnose minnow 0 0 0 0 0 0 0 0 0 0 0 0 0 Brook silverside 4 0 0 0 3 0 0 0 0 0 0 0 7 0,

Brown bullhead 0 27 19 4 0 0 0 -,0 4 0 0 0 53 C, Brown trout .0. 13 8 18 7 42 0 0 0 0 0 19 106 Burbot 7 40 27 35 7 7 0 0 18 52 3 56 253 coi Central 0 0 0 0 0 0 0 0 0 0 0 0 0 I-mudminnow Channel catfish 308 280 174 39 0 0 27 0 0 25 167 34 1,054 Chestnut lamprey 0 0 0 0 0 0 0 0 0 0 0 21 21 Chinook 18 39 17 4 0 13 0 0 4 0 0 0 94 Coho 0 22 12 0 0 7 0 0 0 0 0 0 41 Common carp 0 0 0 0 0 0 13 0 0 0 0 0 13 Deepwater sculpin 0 0 0 0 0 0 0 0 *0 0 0 7 7 Eastern banded 0 0 0 0 0 0 0 0 0 0 0 0 0 killifish Flathead catfish 0 0 0 0 0 0 0 0 0 3 0 14 17 Freshwater drum 0 0 0 0 0 0 0 0 0 0 35 0 35 Gizzard shad 560 1,252 780 0 0 0 0 47 15,682 3,984 3,273 570 26,147 Golden redhorse 0 1 20 4 0 .0 4 -0 0 0 9 0 38 Golden shiner 0 4 0 0 0 0 0 0 0 0 0 0 4 Z Greater redhorse 0 0 0 0 0 0 0 0 0 0 0 0 0 Lake chub 0 0 0 0 0 0 7 0 0 0 0. 0 7 Lake sturgeon 0 0 0 0 0 0 0 0. 0 0 0 0 0 Lake trout 0 0 0 0 14 0 0 0 4 10 9 4 41 0.

Lake whitefish 489 63 9- 25 0 0 11 0 0 78 3,556 750 4,980 Largemouth bass 7 0 0 0 .0 0 0 0 7 0 0 0 14 Longnose dace 0 0 0 0 0 0 0 0 0 0 13 0 13 9 Longnose sucker 70 106 39 81 95 13 0 0 7 0 52 4 466 0 Mottled sculpin 0 0 0 4 11 0 0 0 0 .0 *0 0 14 Ninespine 32 19 22 70 21 0 0 0 0 0 0 7 171 stickleback Northern pike 0 0 0 0 0 0 0 0 0 0 0 0 0 Pumpkinseed 0 0 0 4 0 0 0 0 0 0 0 0 4 Rainbow smelt 780 828 721 355 35 0 24 0 35 81 469 1,666 4,993 (continued)

Table 3-19. (Continued)

N) 2006 2007 Species February] March April J May] June [ July August ] September October [ November December January TOTAL to Rock bass 0 0 0 0 7 0 0 0 0. 0 0 7 14 CO)

CD)

Round goby 432 643 916 7,249 2,180 1,939 827 265 956 1,370 1,945 530 19,251 Sea lamprey 74 14 0 11 0 0 0 0 0 0 0 49 147 M

Shorthead 7 0 0 0 0 0 0 0 0 0 0 0 7 C) redhorse rn Silver redhorse 0 0 0 0 0 0 0 0 7 0 0 0 7 "4 Slimy sculpin 70 37 51 84 42 20 0 0 0. 4 0 0 308 0

Smallmouth bass 7 0 0 0 0 0 0 0 0 4 0 0 11 Spottail shiner 11,169 2,576 1,284 945 152 105 66 133 1,088 1,031 27,121 3,300 48,970 Steelhead 25 .14 19 4 14 0 0 0 0 0 0 25 99 Threespine 385 97 38 75 28 0 0 0 0 14 24 94 755 stickleback Trout-perch 46 30 18 14 0 0 0 0 14 0 150 0 271 Unidentified 0 18 12 0 0 0 0 0 0 0 0 0 30 co Walleye 0 0 0 0 0 0 0 0 0 0 0 0 0 White perch 0 44 29 0 0 0 0 0 0 0 3 0 77 White sucker 11 44 18 77 49 7 0 4 14 0 43 94 360 Yellow perch 352,834 53,174 21,753 12,878 2,041 581 3,850 739 2,274 26,375 163,495 33,727 673,720 TOTAL 367,548 59,701 26,194 23,281 35,604 3,526 5,371 1,352 20,955 38,454 201,669 41,155 824,812 Z

a)

CO CD 0

316(b) PHASE II BASELINE FISH E & I STUDY Table 3-20. Results of Analysis of Variance of Impinged Fish at Cook Nuclear Plant Unit 2.

Data are Loglo (x+l) transformed.

Species Source of Variation I Df MS Pr>F All Species Month(Year) 11 3.47 <0.001 Diel. 1 0.09 , 0.524 Month(Year) X Diel 11 0.07 0.971 Error 84 0.21 Table 3-21. Results of Scheffe's Multiple Comparisons Test among Months for Total Impingement Estimates at Cook Nuclear Plant Unit 2. Months marked with an x in the same row are not significantly different.

Month and Year Dec- T Mar- Jan- Nov- Apr- I May- Jun- Oct- Aug- F Sep-Feb-06 06 06 07 06 06 06 06 06 06 Jul-06 06

___x xxxxxx x x: __ __

x x x x x There were significant differences in impingement of yellow perch among months but not between periods (Table 3-22). Impingement of yellow perch was relatively low during the summer months and highest in February and December (Figure 3-7; Table 3-23). These fish were predominantly from the 2005 year class in February and the 2006 year class in December.

Table 3-22. Results of Analysis of Variance of Impinged Yellow Perch at Cook Nuclear Plant Unit 2. Data are Log1o (x+l) transformed.

Species Source of Variation Df MS I Pr>F All Species Month(Year) 11 6.49 <0.001 Diel 1 0.05 0.716 Month(Year) X Diel 11 0.15 0.942 Error 84 0.36 Table 3-23. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Yellow Perch at Cook Nuclear Plant Unit 2. Months marked with an x in the same row are not significantly different.

Month and Year Dec- Mar- Apr- Nov-i May- Aug- Jun- Oct-i Sep-Feb-06 06 06 J Jan-07 06 06 06 06 07 06 06 Jul-06 x x x x x x x X x x x x X x x x x x x

____________x x x x x x x x x x

_ _ _ _ _x x x x x x 20452 Cook 316b Baseline Final.doc 10/4/07 27 Normandeau Associates, Inc.

316(b) PHASE I/BASELINE FISH E & I STUDY There were significant differences in impingement of spottail shiner among months at Unit 2 but not between diel periods (Table 3-24). Impingement was relatively low from June through September and highest in February and December (Figure 3-7; Table 3-25). Yearling and older fish were dominant in these months.

Table 3-24. Results of Analysis of Variance of Impinged Spottail Shiner at Cook Nuclear Plant Unit 2. Data are Log1o (x+l) transformed.

Species Source of Variation df MS Pr>F All Species Month(Year) 11 7.00 <0.001 Diel 1 0.03 0.753 Month(Year) X Diel 11 0.27 0.586 Error 84 0.31 Table 3-25. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Spottail Shiner at Cook Nuclear Plant Unit 2. Months marked with an x in the same row are not significantly different.

Month and Year Dec-06 Feb- .Jan- 1Mar- 1Apr- Nov- Oct- 1May- Jun- 1 ep- Jul-06 Aug-06 b 07 06 o06 06 06 06 06 P _ An106 x x x x x x x x x x x x

__ _x x x x ___

___x x x x x Similar to yellow perch and spottail shiner, there were significant differences in alewife impingement among months but not between diel periods (Table 3-2 6). Impingement of alewife was relatively low during January through March, and increased dramatically' in May and June, and again in November and December (Figure 3-7; Table 3-27). The*alewife impinged in May and June 2006 were primarily members of the 2005 year class and the alewife impinged in November and December were members of the 2006 year class.

Table 3-26. Results of Analysis of Variance of Impinged Alewife at Cook Nuclear Plant Unit 2.

Data are Loglo (x+1) transformed.

Species Source of Variation I df . MS Pr>F All Species Month(Year) 11 4.58 <0.001.

. Diet 1 I1.03 I0.207 -

Monthear) X Diel 11 0.21 0.978 Error 84 0.63 20452 Cook 316b Baseline Final.doc 10/4/07 28 NormandeauAssociates, Inc.

3';

316(b) PHASE II BASELINE FIsH E & I STUDY Table 3-27. Results of Scheffe's Multiple Comparisons Test among Months for Impingement of Alewife at Cook Nuclear Plant Unit 2. Months marked with an x in the same row are not significantly different.

_Month and Year Jun-06 Nov- Oct- May- Aug-0 Jul-06 Dec- Sep- Jan- Feb- Apr- Mar-06 06 06 06 L_ _106 06 07 06 06 06 X X X . X X X X -X x X x x x _x x x X X X X X X X x X X Impingement data collected from July 2005 through January 2006 and July 2006 through January 2007 provide an opportunity to assess variability, in impingement estimates among comparable months. Total impingement for the pooled seven-month periods was only 1% different between 2005-2006 and 2006-2007 (Table 3-28). However, there were large differences in individual monthly impingement estimates between 2005-2006 and 2006-2007. The greatest variation occurred in September and November due to greater numbers of alewife and yellow perch being impinged in September and November of 2005 compared to the same months in 2006.

Table 3-28. Comparison of fish impingement estimates between July 2005 through January 2006 and July 2006 through January 2007 at Cook Nuclear Plant Unit 2.

Year Jul Aug Sep Oct Nov Dec Jan Total 2005-2006 3,526 5,371 1,352 20,955 38,454 201,669 41,155 312,482 2006-2007 1,056 16,561 21,325 6,357 166,050 28,644 69,159 309,152

% Difference -70 208 1,477 -70 331 -86 68 -1 An estimated 7,091 kg of fish were impinged at Unit 2 from February 2006 through January 2007 (Figure 3-8; Appendix Table K). Impingement of biomass was greatest in December 2006 when 2,140 kg were impinged. Yellow perch (3,532 kg; 50%), lake whitefish (966 kg; 14%) and longnose sucker (499 kg; 7%) contributed most to the total biomass impingement.

3.3 NEARFIELD SAMPLING Sampling of the ichthyoplankton, pelagic fish and demersal fish communities was conducted from June through November in 2005 and April through November in 2006 in the nearfield area of the Cook Nuclear Plant intakes to characterize these communities at various depths and locations.

3.3.1 Ichthyoplankton Sampling Ichthyoplankton sampling in 2005 was conducted June-November and in 2006 from April through November. In June 2005, daytime sampling only was done at the intake and experimental stations and the shoreline station was not sampled. All stations were sampled starting in July 2005.

In general, ichthyoplankton densities were higher at the shoreline station each sampling year (Figure 3-9).

3.3.1.1 Shoreline Station The shoreline station was characterized by higher densities of post yolk-sac larvae compared to other life stages (Table 3-29), and the majority of these were alewife and Cyprinidae (Appendix 20452 Cook 316b3 Baseline Final.doc 10/4/07 29 Normandeau Associates, Inc.

N) Table 3-29. Mean Density (No./100 mi3) of Ichthyoplankton Collected at the Shoreline Station in the Vicinity of Cook Nuclear Plant 0

01 July through November 2005, and April through November 2006.

0 Shoreline CDC April May June July August September October November Average 3 3 3 3 3 3 01 Density/100 mJ Density/100 m Density/100 m Density/100 m Density/100 m Density/100 m Density/100 M3 Density/100 m Density/100 m3 2005 Unidentified 0 0 0 0 0 0 Egg 0 0 0 0 0 0 Yolk-sac larvae 0.995 0 0 0 0 0.199 Cl)

Post-yolk-sac larvae 15.913 0 0 0 0 3.183 qr b.q CA Total 16.908 0 0 0 0 3.382 2006 Unidentified 0 0 0 0 0 0 0 0 0 Egg 0 0 6.881 0 0 0 0 0 0.86 Yolk-sac larvae 2.219 0 25.147 2.363 0 0 0 0 3.716 Post-yolk-sac larvae 0 0.685 18.547 32.303 0.631 0 0 0 6.521 Total 2.219 0.685 50.575 34.666 0.631 0 0 0 11.097 0

0 (A

Co 0

316(b) PHASE II BASELINE FISH E &I STUDY 2500 2000 1500 E

o V

S1000

• 4 E

500 Feb-06 Mar-06 Apr-06 May-06 Jun-06 Jul-06 Aug-06 Sep-06 Oct-06 Nov-06 Dec-06 Jan-07 Month Figure 3-8. Estimated biomass (kg) of fish impinged and biomass composition by month at Unit 2 of Cook Nuclear Plant February 2006 through January 2007.

12 10 T

-e t--

E22005 0

.2 U200600 C

i C

C 0

CL

.2 2-0-

Shoreline Intake, surface Intake, 11 ft Intake, 22 ft Experimental, 0-20 ft Experimental, 30-40 ft Station Figure 3-9. Mean Ichthyoplankton Density (No./100 M 3 ) at the Ichthyoplankton Sampling Stations in the Nearfield Area, April through November 2005 and 2006, off Cook Nuclear Plant.

20452 Cook 316b Baseline Final.doc 10/4/07 31 Normandeau Associates, Inc.

316(b) PHASE!/ BASELINE FISH E & I STUDY Table L). The shoreline station was the only location where fish eggs were captured and these were exclusively alewife.

In 2005 at the shoreline station, ichthyoplankton was only captured in July (Table 3-29). The mean density was 16.9/1OOm 3, most of which was composed of post-yolk-sac larvae (15.9/100 M3 ).

The most commonly captured species were alewives (80%) with Cyprinidae and common carp making up the remainder (Appendix Table L).

In 2006, at the shoreline station highest catches occurred in June and July (Table 3-29). The mean density in June was 50.6/100 mn 3

, made up of yolk-sac larvae (25.1/100 m3), post-yolk-sac larvae (18.5/100 mi ), and eggs (6.9/100 m3 ). The larvae were almost entirely Cyprinidae (99%),

3 while alewife made up the entire egg component (Appendix Table L). In July the mean density was 34.7/100 mi3 , and consisted of mainly of post-yolk-sac larvae (32.3/100 m3), of which 90% were alewife (Appendix Table L). Yolk-sac larvae (2.4/100 m 3) made up smaller component of the total and 64% of these were Cyprinidae (Appendix Table L).

.3.3.1.2 Intake Station The intake station was characterized by relatively equal densities of yolk-sac and post,yolk-sac larvae. Ichthyoplankton densities were lower than the shoreline station and were relatively similar among sampling depths (Figure 3-9). Alewife larvae were the dominant species.

Sampling was conducted in 22 ft of water at the intake station during the day in June 2005 and day and night from July through November 2005 and April through November 2006. During each sampling event samples were collected at the surface, at mid-depth (11 ft) and near the bottom (22 ft).

Among the surface samples ichthyoplankton density was highest in June 2005 (9.2/100 m3) and these were entirely of post-yolk-sac alewife larvae (Table 3-30; Appendix Table L). July was the only other month in 2005 were ichthyoplankton was captured (2.0/100 m3) and these were post yolk-sac alewife larvae (Appendix Table L).

In 2006, the highest density of ichthyoplankton among the surface samples occurred in June (7.3/100 m3 ; Table 3-30). Yolk-sac larvae were dominant,.and these were alewife (45%) and yellow perch (37%) of the total density (Appendix Table L). Post yolk-sac Cyprinidae and yellow perch larvae were also present. Ichthyoplankton also occurred in July (6.9/100 m3) and April (0.9/100 in 3 ).

In July, post yolk-sac alewife larvae were dominant (66%) and in April post yolk-sac round whitefish (100%) were dominant (Appendix Table L).

Ichthyoplankton were present in the mid-depth samples at the intake station in July 2005, and June and July'2006. In July 2005, mean density was 2.0/100 M 3 (Table 3-30) and these were post yolk-sac larvae alewife (50%) and yolk-sac larvae Cyprinidae (50%) (Appendix Table L).

In 2006, ichthyoplankton density among the mid-depth samples was highest in July at 24.3/100 M3 (Table 3-30). Post yolk-sac alewife larvae alewife (53%) and post yolk-sac round goby larvae were dominant (22%) (Appendix Table L). In June 2006, mean density was 3.2/100 m3. Yolk-sac alewife larvae (72%) and post yolk-sac Cyprinidae larvae (28%) occurred (Appendix Table L).

20452 Cook 316b Baseline Final.doc 10/4/07 32 Normandeau Associates, Inc.

~3c

0 W Table 3-30. Mean Density (No./100 mi3 ) of Ichthyoplankton Collected at the Surface, Mid-depth (11 ft) and Bottom (22 ft) of the Intake C) Station in the Vicinity of Cook Nuclear Plant June through November 2005, and April through November 2006.

0PQ 0

Intake, surface C'

co

• April May June July August September October November Total CO)

En

3 Density/100 m3 Density/100 W3 Density/100 m3 Density/100 n3 Density/100 m 3 Density/100 m3 Density/100 n 3 Density/100 i 3 Density/100 W3 rn CD

_n 2005 Unidentified 0 0 0 0 0 0 0 4* Egg 0 0 0 0 0 0 0 03 Yolk-sac larvae 0 0 0 0 0 0 0 CO)

Post-yolk-sac larvae 9.214 1.995 0 0 0 0 1.868 bU. Total 9.214 1.995 0 0 0 0 1.868 0

2006 Unidentified 0 0 0 0 0 0 0 0 0 Egg 0 0 0 0 0 0 0 0 0 Yolk-sac larvae 0 0 5.94 2.307 0 0 0 0 1.031 Post-yolk-sac larvae 0.935 0 1.32 4.615 0 0 0 0 0.859 Co Total 0.935 0 7.26 6.922 0 0 0 0 1.89 Intake, 11 ft April May June July August September. October November Total 3 3 3 3 3 Density/100 ixn Density/100 m3 Density/100 W Density/100 m Density/100 mi Density/100 m Density/100 1w Density/100 1 Density/100 m 2005 Unidentified 0 0 0 0 0 0 0 Egg 0 0 0 0 0 0 0 Yolk-sac larvae 0 0.541 0 0 0 0 0.09 Post-yolk-sac larvae 0 0.541 0 0 0 0 0.09 Total 0 1.081 0 0 0 0 0.18 2006 Unidentified 0 0 0 0 0 0 0 0 0 Egg 0 0 0 0 0 0 0 0 0 Yolk-sac larvae 0 0 2.284- 5.098 0 0 0 0 0.923 Post-yolk-sac larvae 0 0 0.901 19.172 0 0 0 0 2.509 0 Total 0 0 3.185 24.27 0 0 0 0 3.432 Intake, 22 ft April May June July August September October November Total

..... Density/100 ml Density/100-m3 Density/100 M3 Density/100 M3 Density/100 m3 Density/100 m3 Density/1 00 M3 Density/100 m3 Density/100 m3 2005 Unidentified 0 0 0 0 0 0 0 Egg 0 0 0 0 0 0 0 Co Yolk-sac larvae 0 0.953 0 0 0 0 0.159 Co 0 Post-yolk-sac larvae 0 7.654 0 0 0 0 1.276 Total 0 8.607 0 0 0 0 1.434 Q 2006 Unidentified 0 0 0 0 0 0 0 0 0

~5o Egg 0 0 0 0 0 0 0 0 0 S. Yolk-sac larvae 0 0 " 1.642 2.008 0 0 0 0 0.456 Post-yolk-sac larvae 0 0 0 8.944 0 0 0 0 1.118 Total 0 0 1.642 10.952 0 0 0 0 1.574

316(b) PHASE I1BASELINE FISH E & I STUDY In 2006, ichthyoplankton were present in the bottom samples of the intake station in June (1.6/100 m3 ) and July (11.0/100 M3). In June, the catch was made up entirely of yolk-sac alewife larvae. In July, post yolk-sac round goby larvae (64%) were dominant (Appendix Table L).

3.3.1.3 Experimental Station Density of all ichthyoplankton was lower at the experimental station compared to the other stations (Figure 3-9) and post yolk-sac larvae was the dominant lifestage at this station. Alewife and yellow perch larvae were the dominant species at the experimental station.

Sampling was conducted in 40 ft of water at the experimental station from June through November 2005 and April through November 2006. This station was sampled during the day in June 2005 and day and night starting in July 2005. Two composite samples were collected: one containing ichthyoplankton collected from the surface, 10 ft, and 20 ft of depth; and the second from 30 and 40 ft of depth.

Ichthyoplankton were present from the surface to 20 ft in June and July of 2005 and 2006 (Table 3-31). Mean density in June 2005 was (5.7/100 M3) and post yolk-sac yellow perch (44%) and alewife (33%) were dominant (Appendix Table L). In July of 2005 mean density was 4.5/100 M3 post yolk-sac alewife (92%) were dominant.

In 2006, mean density at the surface to 20 ft was highest in July (5.9/100 in 3 ) and ichthyoplankton were also present in June with a mean density of 0.2/100 in 3 . Post yolk-sac alewife larvae were dominant in July (56%) and yolk-sac alewife larvae and post yolk-sac round goby larvae were also present (Appendix Table L). In June, only post yolk-sac yellow perch larvae were present.

At the 40 ft depth of the experimental station, ichthyoplankton occurred in June (7.3/100 in 3) and July (5.4/100 M 3) of 2005 and July of 2006 (2.8/100 M3) (Table 3-30). The June 2005 samples consisted primarily (56%) of post yolk-sac yellow perch larvae (Appendix Table L). The July samples were exclusively post-yolk-sac yellow perch larvae. In July 2006, there were equal densities of yolk sac and post yolk-sac alewife larvae and post yolk-sac round goby larvae (Appendix L).

3.3.1.4 Quantitative Comparisons among Ichthyoplankton Stations Analysis of variance on ichthyoplankton densities was performed to determine if there were significant differences in density among the shoreline, intake, and experimental stations. Sources of variation were sampling station, month and the interaction of these main effects (Table' 3-32). The interaction term was significant indicating that the relationship in ichthyoplankton density between station and month was not consistent. Among the 24 station afid month combinations, ichthyoplankton density was significantly higher at the shoreline'station in June, followed by the shoreline station in July, and the intake station in July of 2006. Ichthyoplankton density at these three station-month combinations was significantly different from each other, and higher than the remaining 21 station-month combinations. There were no significant differences among the remaining 21 station-month combination. This analysis indicates that during the months of high ichthyoplankton abundance density is highest in the shallower waters.

20452 Cook 316b Baseline Final.doc 10/4/07 34 NormandeauAssociates, Inc.

0)

Table 3-31. Mean Density (No.1100 Mi) of Ichthyoplankton Collected at the Surface to Mid-depth (0-20 ft) and Bottom (30-40 ft) of the 0

Experimental Station in the Vicinity of Cook Nuclear Plant June through November 2005, and April through November 2006.

CD, 21 CO Experimental, 0-20 ft CD April May June July August September October November Total 3 3 3 3 C..)

Density/100 W Density/100 mn Density/100 m' Density/100 ml Density/100 m Density/100 mn Density/100 m Density/100 m Density/100 m3

-4 2005 Unidentified 0 0 0 0 0 0 0 :I,.

Egg 0 0 0 0 0 0 0 Yolk-sac larvae 1.265 0.328 0 0 0 0 0.266 Post-yolk-sac larvae 4A27 4.221 0 0 0 0 1.441 Total 5.692 4.549 0 0 0 0 1.707 CO) 2006 Unidentified 0 0 0 0 0 0 0 0 0 PO Egg 0 0 0 0 0 0 0 0 0

-I Yolk-sac larvae 0 0 0 2.375 0 0 0 0 0.297 Post-yolk-sac larvae 0 0 0.241 3.562 0 0 0 0 0.475 Total 0 0 0.241 5.937 0 0 0 0 0.772 Experimental, 30-40 ft r

April t May June July August September Octobe November Total Density/100 ml Density/100 Density/100 Ju me Density/100 m Density/100 A ms Density/100 mb Density/100 ml Density/100 mo Density/100 ml 2005 Unidentified 1.622 ,0 0 0 0 0 0.27 0 Egg 0 0 0 0 0 0 0 Yolk-sac larvae 1.622 0 0 0 0 0 0.27 Post-yolk-sac larvae 4.055 5.398 0 0 0 0 1.575 Total 7.298 5.398 0 0 0 0 2.116 2006 Unidentified 0 -0 0 0 0 0 0 0 0 (A 0 0 0 (A Egg 0 0 0 0 0 0 0

Yolk-sac larvae 0 0 0 0.925 0 0 . 0 0 0.116 Post-yolk-sac larvae 0 0 0 1.85 0 0 0 0 0.231

~5n Total 0 0 0 2.775 0 0 0 0 0.347 p

316(b) PHASE IIBASELINE FISH E & I STUDY Table 3-32. Results of Analysis of Variance of Ichthyoplankton Densities in the Nearfield Area at Cook Nuclear Plant.

Species J Source of Variation Df MS Pr>F All Species Station 2 526.12 <0.001 Month 7 458.22 ' <0.001 Station X Month 14 232.52 <0.001 Error 32 10.57 3.3.2 Gill Net Sampling Gill net sampling was conducted June through November in 2005 and April through November in 2006. Sampling occurred only in the day in June 2005 and day and night after that.

Sampling was conducted at the intake station in 22 ft of water and at the experimental station in 40 ft of water. Gill nets were set at mid-depth at each station. CPUE was higher at the intake station than the experimental station in both years (Figure 3-10). Yellow perch and spottail shiner were the dominant fish at both stations each year (Appendix Table M).

3.3.2.1 Intake Station In 2005 at the intake station, CPUE (fish /hour) was greatest during June (8.2 fish/hr), July (4.3 fish/hr), and September (3.7 fish/hr) (Table 3-32). In each June and September yellow perch.

were the dominant fish (June: 57%; September: 63%). In July, spottail shiner (50%) was the dominant fish. For all months sampled in 2005 yellow perch (43%) and spottail shiner (40%) made the greatest contribution to the overall CPUE. White sucker, rainbow smelt, lake trout, and

.freshwater drum each accounted for 3% of the total CPUE at the intake station in 2005.

In 2006 at the intake station, the CPUE was greatest during the months of October (3.4 fish/hour), July (1.1 fish/hour), and June (1.1 fish/hour) (Table 3-33). The most common species in October and July were spottail shiner (October: 38%; July: 55%). In June, longnose sucker were most common (55%). Two lake sturgeon, a state threatened species in Michigan, were captured during the month of August but did not make up a large percentage of the total CPUE in 2006.

Spottail shiner (40%) and yellow perch (24%) were the dominant fish at the intake station in 2006..

3.3.2.2 Experimental Station In 2005 at the experimental station, CPUJE was highest in the months of July (4.4 fish/hr),

October (1.2 fish/hr), and August (1.1 fishihr) (Table 3-33). The majority of the catch in July were yellow perch (57%) and spottail shiner (34%), while the largest component of the October catch were alewife (75%) and the August catch were mostly yellow perch (45%) and rainbow smelt (36%).

Yellow perch (42%) and spottail shiner (25%) were the dominant fish at the intake station in 2005.

In 2006 at the experimental station, CPUE was highest during the months of July (1.3 fish/hour), October (0.9 fish/hour), and August (0.8 fish/hour) (Table 3-33). Spottail shiner (53%),

yellow perch (15%), and white sucker (15%) were major components of the July catch. In October, yellow perch (56%) and freshwater drum (33%) made up the majority of the catch while the most

• common species in August were spottail shiner (56%) and yellow perch (44%). Spottail shiner (40%)

and yellow perch (40%) were the dominant species at the experimental station in 2006.

20452 Cook 316b Baseline Final.doc 10/4/07 36 Normandeau Associates, Inc.

Lq3

316(b) PHASE II BASELINE FISH E & I STUDY 3.5 3,5.

'C 2.5 0

-u 2 0.

U S1.5 Intake Expenmental Station Figure 3-10. Mean Catch per Unit Effort (fish/hour) at the Gill Net Sampling Stations in the Nearfield Area, April through November 2005 and 2006, off Cook Nuclear Plant.

3.3.2.3 Quantitative Comparisons among Gill Net Stations Analysis of variance on gill net CPUE was performed to determine if there were significant differences in CPUE between the intake and experimental stations. Sources of variation were month and sampling station and the interaction of these main effects. The overall ANOVA model was not significant, but it did explain 43% of the variability in CPUE. Although the main effects were not significant, overall CPUE (pooled across stations) was highest in October (2.14 fish/hour) followed by July (1.20 fish/hour) and August (0.67 fish/hour). Similarly overall CPUE (pooled across months) was higher at the intake station (0.99 fish/hour) compared to the experimental station 0.51 (fish/hour).

3.3.3 Otter Trawl Sampling Otter trawl sampling was conducted at the intake station (22 ft of water) and experimental stations (40 ft of water) June through November in 2005 and April through November in 2006.

Sampling at the intake and experimental stations occurred only in the day in June 2005 and day and night after that. Sampling at a shoreline station (5 ft of water) started in October 2005 and was conducted day and night each sampling month after that. CPUE was highest at the intake station followed by the experimental and shoreline station (Figure 3-11). Yellow perch and round goby were the most common fish captured (Appendix Table N).

3.3.3.1 Shoreline Station During the two months of sampling at the shoreline station in 2005, fish were only captured during the month of October (Table 3-34). Mean CPUE was 12 fish/tow with the main catch 20452 Cook 316b Baseline Final.doc 10/4/07 37 Normandeau Associates,Inc.

Lt4q

316(b) PHASE II BASELINE FISH E & I STUDY Table 3-33. Mean Catch per Unit Effort (fish/hour) for Fish Captured in the Gill Net at the Intake Station (22 ft) and Experimental Station (40 ft) in the Vicinity of Cook Nuclear Plant, June through November 2005 and April through November 2006.

Intake Station April May] June] July I August September October November Total CPUE CPUE j CPUE CPUE CPUE CPUE CPUE CPUE CPUE 2005 Alewife 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Bloater 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Channel 0.00 0.00 0.00 0.00 0.00 0.00 0.00 catfish Common carp 0.00 0.00 0.00 0.12 0.12 0.00 0.04 Freshwater 0.00 0.00 0.00 0.12 0.37 0.00 0.08 drum Gizzard shad 0.00 0.00 0.00 0.24 0.00 0.00 0.04 Golden 0.00 0.00 0.00 0.00 0.00 0.12 0.02 redhorse Lake sturgeon 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Lake trout 0.00 0.00 0.36 0.00 0.00 0.00 0.06 Lake whitefish 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Longnose 0.12 0.00 0.00 0.00 0.00 0.00 0.02 sucker Rainbow smelt 0.00 0.00 0.59 0.00 0.00 0.23 0.14 Round goby 0.00 0.00 0.00 0.00 0.00 0.24 0.04 Spottail shiner ' 2.88 2.17 0.47 0.86 0.49 1.30 1.36 Walleye 0.00 0.12 0.12 0.00 0.00 0.00 0.04 White sucker 0.56 0.00 0.12 0.00 0.00 0.00 0.11 Yellow perch 4.67 2.05 0.24 2.33 0.00 0.00 1.55 Total 8.23 4.34 1.89 3.67 0.98 1.91 3.50 2006 Alewife 0.00 0.00 0.13 0.00 0.00 0.00 0.00 0.00 0.02 Bloater 0.00 0.00 0.00 0.00 0.00 0.00 0.13 0.13 0.03 Channel 0.00 0.00 0.00 0.00 0.00 0.00 0.26 0.00 0.03 catfish Common carp 0.00 0.00 000 0.00 0.00 0.00 0.00 0.00 0.00 Freshwater 0.00 0.00 0.00 0.00 0.00 0.12 0.40 0.00 0.06 drum Gizzard shad 0.11 0.00 0.00 0.00 0.00 0.00 0.26 0.00 0.05 Golden 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 redhorse Lake sturgeon 0.00 0.00 0.00 0.00 0.25 0.00 0.00 0.00 0.03 Lake trout 0.00 0.00 0.00 0.08 0.00 0.00 0.00 0.12 0.03 Lake whitefish 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.12 0.02 Longnose 0.00 0.00 0.60 0.00 0.00 0.00 0.00 0.00 0.08 sucker Rainbow smelt 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.08 0.00 0.00 0.00 0.00 0.01 Spottail shiner 0.11 0.25 0.36 0.58 0.00 0.36 1.32 0.12 0.39 Walleye 0.00 0.00 0.00 0.00 0.13 0.00 0.00 0.00 0.02 White sucker 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Yellow perch 0.00 0.00 0.00 0.36 0.12 0.36 1.06 0.00 0.24 Total 0.23 0.25 1.09 1.10 0.50 0.84 3.42 0.50 0.99 (continued) 20452 Cook 316b Baseline Final.doc 10/4/07 38 NormandeauAssociates, Inc.

316(b) PHASE HI BASELINE FISH E & I STUDY Table 3-33. (Continued)

Experimental Station April May June July August September October November Total CPUE CPUE {CPUE CPUE CPUE CPUE I CPUE ] CPUE " CPUE 2005 Alewife 0.00 0.00 0.00 0.00 0.87 0.00 0.14 Bloater 0.00 0.00 0.12 0.00 0.25 0.00 0.06 Channel catfish 0.00 0.25 0.00 0.00 0.00 0.00 0.04 Common carp 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Freshwater drum 0.00 0.00 0.00 0.00 0.12 0.00 0.02 Gizzard shad 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Lake trout 0.24 0.00 0.12 0.00 0.00 0.00 0.06 Lake whitefish 0.12 0.00 0.00 0.00 0.00 0.00 0.02 Longnose sucker 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 0.38 0.00 0.00 0.00 0.06 Round goby 0.00 0.13 0.00 0.00 0.00 0.00 0.02 Spottail shiner 0.00 1.50 0.00 0.00 0.00 0.25 0.29 Walleye 0.00 0.00 0.00 0.00 0.00 0.12 0.02 White sucker 0.00 0.00 0.00 0.00 , 0.00 0.00 0.00 Yellow perch 0.00 2.50 0.50 0.00 0.00 0.00 0.50 Total 0.37 4.38 1.11 0.00 1.24 0.37 1.24 2006 Alewife 0.00 0.00 0.00 0.12 0.00 0.00 0.00 0.00 0.01 Bloater 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.12 0.02 Channel catfish 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Common carp 0.00 0.00 0.00 1 0.00 0.00 0.00 0.00' 0.00 0.00 Freshwater drum 0.00 0.00 0.00 0.00 0.00 0.00 0.25 0.00 0.03 Gizzard shad 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Lake trout 0.11 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 Lake whitefish 0.00 0.00 0.00 0.00 0.00 -0.00 0.00 0.12 0.02 Longnose sucker 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.12 0.02 Rainbow smelt 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Spottail shiner 0.00 0.00 0.00 0.71 0.47 0.12 0.13 0.12 0.19 Walleye 0.00 0.00 0.00' 0.00 0.00 0.00 0.00 0.12 0.02 White sucker 0.00 0.00 0.00 0.24 0.00 0.00 0.00 0.00 0.03 Yellow perch 0.00 0.00 0.00 0.24 0.38 0.12 0.50 0.12 0.17 Total 0.11 0.00 0.00 1.30 0.84 0.24 0.88 0.72 0.51 20452 Cook 316b Baseline Final.doc 10/4/07 39 Normandeau Associates, Inc.

316(b) PHASE II BASELINE FISH E & I STUDY Table 3-34. Mean Catch per Unit Effort (fish/trawl) for Fish Captured in the Otter Trawl at the Shoreline Station (5 ft) Intake Station (22 ft) and Experimental Station (40 ft) in the Vicinity of Cook Nuclear Plant, June through November 2005 and April through November 2006.

5 ft depth April May June July August September October November Total 2005 Alewife 0.5 0.0 0.3 Bloater 1.5 0.0 0.8 Gizzard shad 8.5 0.0 4.3 Lake whitefish 0.0 0.0 0.0 Rainbow 0.0 0.0 0.0 smelt Round goby 0.0 0.0 0.0 Slimy sculpin 0.0. 0.0 0.0 Spottail shiner 0.5 0.0 0.3 White sucker 0.0 0.0 0.0 Yellow perch 1.0 0.0 .0.5 Total 12.0 0.0 6.0 2006 Alewife 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0:0 0.0 0.0 1 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Rainbow 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 smelt Round goby 0.0 0.5 0.0 0.0 0.0 0.0 0.0 0.0 0.1 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner 0.5 2.5 0.0 0.0 0.5 0.0 0.5 0.5 0.6 White sucker 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Yellow perch 0.5 1.0 1.0 25.0 0.0 0.0 0.0 0.5 3.5 Total 1.0 4.0 1.0 25.0 0.5- 0.0 0.5 1.0 4.1 20452 Cook 316b Baseline Final.doc 10/4/07 .40 NormandeauAssociates, Inc.

316(b) PHASE II BASELINE FISH E & I STUDY Table 3-34. (Continued)

Intake April May [June July August September October November [ Total 2005 Alewife 2.0 0.0 1.5 0.0 0.5 0.0 0.7 Bloater 0.0 0.0 0.0 0.0 1.5 0.0 0.3 Gizzard shad 0.0 0.0 0.0 0.0 1.5 0.0 0.3 Lake 0.5 0.0 0.0 0.5 0.0 0.0 0.2 whitefish Rainbow 0.0 0.0 0.0 0.0 0.0 0.0 0.0 smelt Round goby 2.0. 0.0 0.0 0.0 0.0 0.0 0.3 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner 8.5 0.5 2.0 0.0 1.0 0.5 2.1 White, sucker 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Yellow perch 5.0 0.0 0.5 49.5 5.5 2.0 10.4 Total 18.0 0.5 4.0 50.0 10.0 2.5 14.2 2006 Alewife 0.0 0.0 0.0 .1.5 0.0 0.0 0.0 0.0 0.2 Bloater 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Lake 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 whitefish Rainbow 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 smelt Round goby 0.0 0.5 0.0 0.5 0.0 0.0 0.0 0.0 0.1 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner 0.0 0.5 1.5 1.0 0.0 0.0 0.0 0.5 0.4 White sucker 0.0 0.0 0.5 0.0 0.0 0.0 0.0 0.0 0.1 Yellow perch 1.5 7.0 33.5 56.0 1.0 0.5 0.5 0.5 12.6 Total 1.5 8.0 35.5 59.0 1.0 0.5 0.5 1.0 13.4 20452 Cook 316b Baseline Final.doc 10/4/07 41 NormandeauAssociates, Inc.

L+8

3 16(b) PHASE II BASELINE FISH E & I STUDY Table 3-34. (Continued) 40 ft April May June July August September October November Total depth I I I 2005 Alewife 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 4.5 0.0 0.8 Gizzard 0.0 0.0 0.0 0.0 0.0 0.0 0.0 shad Lake 0.0 0.0 0.0 0.0 0.0 0.0 0.0 whitefish Rainbow 0.0 0.0 0.0 0.0 0.0 0.0 0.0 smelt Round goby 0.0 0.0 2.0 2.0 0.5 0.0 0.8 Slimy 0.0 0.0 0.0 0.0 0.0 0.0 0.0 sculpin Spottail 0.0 0.0 0.0 0.0 0.0 0.0 0.0 shiner White 0.0 0.0 0.0 0.0 0.0 0.0 0.0 sucker Yellow 1.0 1.0 0.0 0.0 11.0 0.0 2.2 perch Total 1.0 1.0 2.0 2.0 16.0 0.0 3.7 2006 Alewife 0.0 0.0 0.0 2.5 0.0 0.0 0.0 0.0 0.3 Bloater 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Gizzard 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 shad Lake 0.0 2.0 1.0 2.5 0.0 0.0 0.0 0.0 0.7 whitefish Rainbow 0.5 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.2 smelt Round goby 1.0 2.5 4.5 8.0 1.5 0.5 0.0 0.0 2.3 Slimy 0.5 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 sculpin Spottail 1.0 0.5 0.5 2.0 0.0 0.0 0.0 4.0 1.0 shiner White 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 sucker Yellow 2.5 5.0 0.5 2.0 0.5 1.0 0.5 2.5 1.8 perch Total 5.5 11 6.5 17 2 1.5 0.5 6.5 6.3 20452 Cook 316b Baseline Final.doc 10/4/07 42 Normandeau Associates, Inc.

316(b) PHASE II BASELINE FISH E & I STUDY 16 14 12

=_ 10 . . . .. . . . . . . . ..

a IL 220 0

Shore Intake Experimental Station Figure 3-11. Mean Catch per Unit Effort (fish/trawl) at the Trawl Sampling Stations in the Nearfield Area, April through November 2005 and 2006, off Cook Nuclear Plant.

Sampling in 2006 took place from April-November. At the shoreline station the highest mean CPUE occurred during July (25 fish/trawl), made up entirely of yellow perch (Table 3-33). The next highest CPUE occurred in May (4 fish/trawl) with spottail shiner (63%), yellow perch (25%),

and round goby (13%) the only species caught.

Yellow perch were the dominant fish at this station accounting for 85% of the total CPUE. A smaller component of the catch was made up of spottail shiner and round goby.

3.3.3.2 Intake Station In 2005 at the intake station, the highest mean CPUE occurred during the months of September (50.0 fish/trawl), June (18.0 fish/trawl), and October (10 fish/trawl) (Table 3-34). Yellow perch were dominant in September catch (98%) as well as the October catch (55%). The June catch contained mostly spottail shiners (47%) and yellow perch (28%). Yellow perch were the dominant fish at the intake station in 2005, making up 73% of the total CPUE. Spottail shiners were the second most common catch at 15% of the total. Other species contributing to the total CPUE were: alewives (5%), bloaters (2%), gizzard shad (2%), round goby (2%), and lake whitefish (1%).

In 2006, mean CPUE at the intake station was highest during July (59.0 fish/trawl), June (35.5 fish/trawl), and May (8 fish/trawl) (Table 3-33). Yellow perch was the largest component of the catch in each month (July: 95%; June: 94%: May: 88%).

Yellow perch were also the largest contributor to the overall mean CPUE in 2006, accounting for 94% of the total. Spottail shiner (3%), alewives (1%), round goby (1%), and white sucker (1%)

also made up smaller components of the total catch.

20452 Cook 316b Baseline Final.doc 10/4/07 43 NormandeauAssociates, Inc.

go

316(b) PHASE I1BASELINE FISH E & I STUDY 3.3.3.3 Experimental Station In 2005, CPUE at the experimental station was greatest during October (16 fish/trawl) with the majority of the catch consisting of yellow perch (69%) and bloaters (28%) the only fish captured (Table 3-34). The next highest catch rates occurred in August (2.0 fish/trawl) and September (2.0 fish/trawl) with round goby as the only component of each monthly mean CPUE. The species composition in. 2005 at the experimental station was yellow perch (58%) round goby (22%) and boater (22%)

In 2006, the highest CPUE at the experimental station was 17 fish/trawl in July, 11 fish/trawl in May, and 6.5 fish/trawl in both June and November (Table 3-33). In July, the dominant fish was round goby (47%) followed by alewife (15%) and lake whitefish (15%). The May CPUE consisted primarily of yellow perch (45%), round goby (23%), and lake whitefish (18%) and the June CPUE consisted of round goby (69%) and lake whitefish (15%). In November the CPUE was dominated by spottail shiner (62%) and yellow perch (38%).

Overall, all sampling months in 2006, round goby (37%), yellow perch (29%), and spottail shiners (16%) were the dominant species. A smaller component of the total CPUE at this site consisted of lake whitefish (11%), alewives (5%), and rainbow smelt.

3.3.3.4 Quantitative Comparisons among Otter Trawl Stations Analysis of variance on otter trawl loglo (x+1) CPUE was performed to determine if there were significant differences in CPUE between the shoreline, intake, and experimental stations.

Sources of variation were month and sampling station and the interaction of these main effects. There were significant ,differences among months and stations (Table 3-35). CPUE was highest in April through July and November and there were no significant differences among these months (Table 3-36). Similarly there were no significant differences in CPUE among April through June, and August through November.

When the data were logl 0 (x+l) transformed there were significant differences in CPUE among stations (Table 3-37). There were no significant differences between the experimental station and the intake station, and between the intake and shore stations.

Table 3-35. Results of Analysis of Variation of Trawl CPUE in the Nearfield Area of Cook Nuclear Plant. Data are Loglo (x+l) transformed.

Species Source of Variation Df I MS Pr>F All Species Station 2 0.69 0.0004

[ Month 7. 0.91 0.0201 Station X Month 14 0.13 0.6050 Error 24 0.15 20452 Cook 316b Baseline Final.doc 10/4/07 44 Normandeau Associates, Inc.

5-I

316(b) PHASE II BASELINE FISH E & I STUDY Table 3-36. Results of Scheffe's Multiple Comparisons Test among Months for Otter Trawl CPUE (catch per trawl) in the Nearfield Area of Cook Nuclear Plant. Months marked with an x in the same row are not significantly different.

Sampling Month Jul-06 May- Jun- Apr- Nov- Aug- Oct- Sep 06 06 06 06 06 06 06 XX x x X *X x X x x x x IiX I

Table 3-37. Results of Scheffe's Multiple Comparisons Test among Sampling Stations for Otter Trawl CPUE (catch per trawl) in the Nearfield Area of Cook Nuclear Plant.

Stations marked with an x in the same row are not significantly different.

3.3.4 Seine Sampling Seine sampling in 2005 occurred from August-November (Table 3-38; Appendix Table 0).

During that time, highest mean CPUJE occurred during August (334.5 fish/haul) and October 45.8 fish/haul). The August catch was composed mostly of yellow perch (306.8 fish/haul) and spottail shiner (15.5 fish/haul) and the October catch was composed primarily of gizzard shad (30.3 fish/haul) and bloater (8.8 fish/haul).

Yellow perch were the most common fish captured species in 2005 accounting for 78% of the total CPUE (Table 3-38) with the majority being captured in August. Gizzard shad were the second most commonly captured species accounting for 10% of the total CPUE, and the majority of these were captured in October. Species making up a smaller component of the 2005 total CPUE were spottail shiner (5%), alewife (3%) and bloater (3%).

In 2006, seine sampling took place from April-November and mean CPUE was greatest during the months of June (57.5 fish/haul) and October (39.5 fish/haul) (Table 3-37; Appendix Table 0). The June catch was composed mostly of spottail shiners (44.5 fish/haul) and to a lesser degree yellow perch (4.8 fish/haul). The largest component of the October catch was bloater (20.3 fish/haul) and alewife (16.0 fish/haul).

Spottail shiners made up the largest component of the seine sampling in 2006 contributing 54% to the total CPUE. Spottail shiners were captured during each month of sampling in 2006 and the largest catches occurred in June (44.5 fish/haul) and May (16 fish/haul). Alewife contributed 15%

to the total CPUE catch with the largest catches in October (16.0 fish/haul) and July (3:3 fish/haul).

Bloater contributed 14% to the total CPUJE with the largest catches occurring in October (20.3 fish/haul).

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316(b) PHASE II BASELINE FISH E & I STUDY Table 3-38. Mean Catch per Unit Effort (fish/haul) for Fish Captured in the Seine at the Shore Station in the Vicinity of Cook Nuclear Plant, June through November 2005 and April through November 2006.

Year Species Apri]l May June [ July ]AugustSeptemberlOctober[NovemberJ Total 2005 Alewife 9.80 0.00 3.80 0.30 3.40 Bloater 1.50 0.30 8.80 0.00 2.60 Bluegill 0.00 0.00 0.30 0.00 0.10 Brook silverside 0.00 0.00 0.00 0.00 0.00 Chinook 0.00 0.00 0.00 0.00 0.00 Coho 0.00 0.00 0.00 0.30' 0.10 Eastern banded killifish 0.50 0.30 0.30 0.00 0.30 Gizzard shad 0.00 0.30 30.30 7.80 9.60 Lake trout 0.00 0.00 0.00 0.30 0.10 Longnose sucker 0.00 0.00 0.00 0.30 0.10 Muskellunge 0.00 0.00 0.30 0.00 0.10 Rainbow smelt 0.00 0.00 0.00 0.00 0.00 Round goby 0.50 0.00 0.00 0.00 0.10 Shorthead redhorse 0.00 0.00 0.00 0.00 0.00 Spottail shiner 15.50 0.30 '2.00 2.30 5.00 Steelhead 0.00 0.00 0.00 0.00 0.00 Walleye 0.00 0.00 0.00 0.00 0.00 White sucker 0.00 0.00 0.00 0.00 0.00 Yellow perch 306.80 0.00 0.30 0.00 76.80 TOTAL 1334.50 1.00 45.80 11.00 98.10 2006 Alewife 0.00 0.00 2.50 3.30 0.30 0.00 16.00 0.00 2.80 Bloater 0.00 0.00 0.50 0.00 0.00 0.00 20.30 0.30 2.60 Bluegill ,0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Brook silverside 0.80 0.30 0.00 0.00 0:00 0.00 0.00 0.00 0.10 Chinook 0.00 0.00 0.00 0.30 0.00 0.00 0.00 0.00 0.00 Coho 0.00 0.00 0.50 0.00 0.00 0.30 0.00 0.00 0.10 Eastern banded killifish 0.00 0.00 0.50 0.00 0.00 0.00 0.00 0.00 0.10 Gizzard shad 0.30 0.00 0.00 0.00 0.00 0.00 0.30 0.00 0.10 Lake trout 0.00 0.00 0.00 0.00 0.00 0.00 0.30 0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Muskellunge 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 4.00 0.00 0.00 0.00 0.30 0.00 0.50 Round goby 3.50 1.30 0.00 0.50 0.30 0.00 0.00 0.00 0.70 Shorthead redhorse 0.00 0.00 0.00 0.00 0.00 0.00 0.50 0.00 0.10 Spottail shiner 2.50 16.00 44.50 12.30 0.30 1.30 2.00 0.80 9.90 Steelhead 0.00 0.00 0.00 0.30 0.00 0.00 0.00 0.00 0.00 Walleye 0.00 0.00 0.30 0.00 0.00 0.00 0.00 0.00 0.00 White sucker 0.50 0.00 0.00 0.30 0.00 0.00 0.00 0.00 0.10 Yellow perch 0.80 0.00 4.80 2.00 1.00 0.80 0.00 0.30 1.20 TOTAL 8.30 17.50 57.50 18.80 1.80 2.30 39.50 1.30 18.30 20452 Cook 316b Baseline Finaldoc 10/4/07 46 Normandeau Associates, Inc.

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316(b) PHASE II BASELINE FISH E & I STUDY 3.4 WATER QUALITY Water quality data (surface and bottom temperature and dissolved oxygen, and were dollected with each nearshore fisheries sample. None of the water quality parameters were within ranges that would affect fish distribution, and there was not obvious association between water quality and impingement and entrainment rates.

3.4.1 Ichthyoplankton.Water Quality Sampling Water quality data was collected in association with ichthyoplankton sampling at the three sampling stations. Surface water quality was collected at the shoreline station,.and also at each samplingdepth at the intake (22 ft) and experimental (40 ft) stations (Appendix table P). Water temperatures were generally highest at the surface stations declining with depth, except in April 2006 where water temperatures at the intake and experimental depth were uniform throughout. The highest water temperature recorded was 27. PC at the surface of the intake station in July 2005. The lowest temperature.recorded was 6. I°C at the experimental station in April, 2006.

Dissolved Oxygen levels (mg/1) were generally greater early in the sampling season and tended to increase with depth and declining water temperatures. The highest measured levels of dissolved Oxygen occurred at the 30-40 ft depth of the experimental station (13.5 mg/I) in June, 2006.

The lowest occurred at the surface-20 ft depth at the experimental station (8.0 mg/l) in July 2005.

Neither water temperature nor dissolved oxygen levels were limiting to fish distribution.

3.4.2 Gill Net Water Quality Sampling Water quality data collected in association with the gill net sampling at, the intake (22 ft) and experimental (40 ft) stations (appendix Table Q). Water temperatures at both the intake and experimental stations were lowest in the April of 2006 (6.0'C) and increased to the annual high in August (22.99C). The seasonal pattern in dissolved oxygen levels was opposite to that of temperatures with the highest dissolved oxygen levels occurring in April (12.7 mg/!) and the lowest in September (8.4 mg/1) when water temperatures were still relatively high. Neither water temperature nor dissolved oxygen levels were limiting to fish distribution.

3.4.3 Otter Trawl Water Quality Sampling Water quality data were collected in association with the otter trawl sampling at the three stations. Surface water quality data were collected at the 5 ft station, and surface and bottom data were collected at the Intake (22 ft) and Experimental (40 ft) stations (Appendix Table Q). In general, water temperatures were most extreme at the surface. The warmest water temperature recorded was 27.1 'C at the surface at the intake station and the coldest temperature of 6.1 'C occurred at the experimental station at the surface. The lowest levels of dissolved oxygen (8.6 mg/l) occurred in September at the surface of the intake station and at the surface and bottom of the experimental station in September. The highest level of dissolved oxygen occurred at the surface of the experimental station in April when water temperatures were relatively cool. Neither water temperature nor dissolved oxygen levels were limiting to fish distribution.

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316(b) PHASE II BASELINE FISH E & I STUDY 3.4.4 Seine Water Quality Sampling Water Quality data was collected in association with the seine sampling at the surface (Appendix Table Q). Water temperatures ranged from 25.0 'C in September 2005 to 8.5 'C in April 2006.

Dissolved oxygen ranged form 8.8 mg/1 in September 2005 to 12.5 mg/1 in April 2006. Both water temperature and dissolved oxygen were within limits that would not affect fish distribution.

3.5 QUALITY ASSURANCE AND QUALITY CONTROL (QA/QC) RESULTS Acomprehensive Quality Assurance and Quality Control (QA/QC) program (see Section 2.5) was instituted during this study. Audits of field procedures were conducted twice during the program.

The first audit was conducted in September 2005 by AEP personnel and resulted in the start of otter trawl sampling at the shoreline station in October 2005. The second audit occurred in January 2007 and confirmed that all field sampling procedures were in conformance with the SOP.

The laboratory analysis of entrainment and ichthyoplankton samples was subject to a MIL-STD 1235B single and multiple level continuous sampling procedures and tables for inspection by attributes for the tasks of sorting and identification that resulted in an AOQL of 10% or better. In this plan, eight samples in a row for each analyst were reinspected and if all eight samples passed QC, then I out 7 samples would be randomly selected for reinspection. Any samples that failed QC were reanalyzed. A total of 72 samples were reinspected for sorting with one failure, and 27 samples were reinspected for identification with no failures.

All data files were also subject QC reinspection that resulted in data sets with an AOQL of 1% or better. Lot sampling plans based on the number of records in the data file (American Society for Quality Control 1993) were used to determine the number of data records for reinspection, and the number of incorrect data records that would result ina failed data file. Each randomly selected data record was then verified against original field or lab data sheets. If a data file failed QC it was either subject to 100% inspection if the failure was due to random error, or recreated if the failure was due to a systematic error. All data files passed QC on the first audit.

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316(b) PHASE II BASELINE FISH E & I STUDY 4.0 DiscusSION The University of Michigan conducted extensive investigations into the impact of the operation of Cook Nuclear Plant on southeastern nearshore Lake Michigan (UM 1986). The impingement and entrainment studies conducted by UM from 1978 to 1982 when both units were operational provide the best available data for comparison with the present study. However, there were important differences in the methodology and taxonomy between the two studies. The UM study did not attempt to identify fish eggs to the species level, although it is likely that the majority were alewife. Similarly, in the present study we did not make major effort to distinguish among minnow (Cyprinidae) larvae, although the majority were probably spottail shiner. Furthermore, the UM entrainment study did not identify any specimens as either YOY or older lifestages although they probably were present. In the UM study entrainment estimates were based on the annual number of fish eggs and larvae entrained and actual cooling water, flow, which ranged from 1,138 million m3 in 1977 to 2,830 m3 in 1980. The present study assumed that cooling water system was in full operation all year and annual cooling water flow was 3,322 m 3 per year. Despite these differences between the studies, valid comparisons between the time periods can be made.

Fish eggs were much more abundant in 1978 through 1982 than in the present study (Table 4-1). Alewife are one of the few species that have pelagic eggs in Lake Michigan and with the increased abundance of alewife in the 1970s and 1980s, it is likely that the majority of the eggs entrained in 1978 through 1982 were alewife. Alewife were the most abundant eggs entrained in this study, although density was much lower than the UM study probably due to the current decreased stock size of alewife.

Table 4-1. Average number of fish eggs entrained at Cook Nuclear Plant between 1978 and 1982, and in the present study.

Annual Average Number of Eis Entrained (in millions)

Species 1978-1982a Feb. 2006-Jan. 2007b Alewife 7.08 Cyprinidae 4.42 Unidentified 3,713.85 3.78 Common carp 1.58 TOTAL 3,713.85 16.86 Eggs/10 6 m 3 1.41 0.025 a Entrainment estimates are based on actual cooling water flow.

b Entrainment estimates are based on design cooling water flow.

The average annual number of larvae entrained was relatively similar between the two studies. An average of 115 million larvae were entrained annually between .1978 and 1982, while 83 million larvae were entrained in this study (Table 4-2). However, when standardized for cooling the entrainment rates of both fish eggs and larvae indicate that entrainment is not always proportional to cooling water flow. When standardized for cooling water flow, there were orders of magnitude differences in both fish egg and larval annual entrainment rates between 1978-1982 and the present.

The differences in entrainment estimates between July 2005 through January 2006, and July 2006 through January 2007 also illustrate the effect of the abundance of fish eggs and larvae in the 20452 Cook 316b Baseline Final.doc 10/4/07 49 Normandeau Associates, Inc.

316(b) PHASE IIBASELINE FISH E & I STUDY Table 4-2. Annual Average number of fish larvae entrained at Cook Nuclear Plant between 1975 and 1982 and in the present study.

Annual Average Number of Larvae Entrained (in millions)

Species 1978-1982a Feb. 2006-Jan. 2 0 0 7 b Alewife 82.01 12.38 Spottail shiner 12.01 0.00 Rainbow smelt 6.77 9.26 Yellow perch 2.36 0.30 Trout-perch 0.61 0.00 Round goby 0.00 44.04 Cyprinidae 0.46 16.87 Others 11.16 0.97 TOTAL 115.38 83.82 Larvae/106 m3 0.04 0.005 "Entrainment estimates are based on actual cooling water flow.

b Entrainment estimates are based on design cooling water flow.

withdrawal waters on entrainment (Table 3-5). These data indicate that the abundance of the fish eggs and larvae in the withdrawal waters is an important factor in fish egg and larvae entrainment.

The seasonality of larval entrainment was similar between the two studies. Entrainment typically began in April and peaked in June and July when alewife and Cyprinidae larvae (both studies) and round goby larvae (present study) were most abundant.

The UM study found a significant differences in larval entrainment between diel periods.

Entrainment was greater in the dusk to midnight and midnight to dawn periods than during the day (UM 1986). The present study supports that finding to some degree, although it is not possible to fully evaluate the UM findings because the ANOVA results are not presented. In the present study entrainment was greater at night, although there was a significant interaction between diel period and month which indicated that this relationship was not consistent in all months.

Table 4-3. Annual Average number of fish Impinged at Cook Nuclear Plant (Units 1 and 2) between 1975 and 1982 and in the present study.

Annual Average Number of Fish Impinged Species 1978-1982a Feb. 2006-Jan. 2 0 0 7b Alewife 926,241 82,705 Spottail shiner 91,376 87,320 Yellow perch 134,439 1,116300 Trout-perch 32,093 536 Rainbow smelt 72,439 8,990 Slimy sculpin 4,964 1,010 Round goby 11 0 35,180 Gizzard shad 1,444 34,374 Others 13,965 19,608 TOTAL 1,252,965 1,386,023 a Impingement estimates are based on actual cooling water flow.

b Impingement estimates are based on design cooling water flow.

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316(b) PHASE IIBASELINE FISH E & I STUDY The trends in annual impingement reflect the trends in annual abundance of fish in Lake Michigan. The decrease in alewife impingement can be attributed to the decline in alewife abundance in Lake Michigan since 2002 (Bunnell et al. 2007), and the operation of the acoustic fish deterrence system at Cook Nuclear Plant. The reduction in rainbow smelt abundance in impingement samples can also be attributed to a decrease in stock size since its peak in 1981-1993 (Bunnell et al. 2007).

The increased impingement of yellow perch in the present study can be attributed to the record 2005 year class. Round goby were not present in Lake Michigan in 1978-1982 and are presently increasing in abundance (Bunnell et al. 2007), which accounts for their presence in impingement in the present study. Gizzard shad was first reported from Lake Michigan in 1953 (Miller 1957) and it may be more abundant now than in 1978-1982, as indicated by the large increase in gizzard shad impingement.

The temporal variability in impingement is also indicated by a comparison of impingement estimates between July 2005 through January 2006, and July 2006 through January 2007. At Unit 1 there was 63% difference in the impingement estimates for the two time periods and at Unit 2 there was only a 1% difference between the time periods (Tables 3-18 and 3-27). However, at both units the monthly estimates varied widely, and in the case of Unit 2, positive and negative variations almost cancelled each other out. These results and the comparisons with the UM studies indicate that there is a large degree of temporal variation in impingement estimates, and impingement may not be proportional to cooling water flow.

Further indications of the temporal variability in impingement is indicated by a comparison of monthly impingement and species composition between 1978-1982 and the present study. In 1978-1982, impingement was highest in April through October and was attributed to an inshore movement of fish in the spring followed by an offshore movement in the fall (UM 1986). In the present study impingement was greatest in February 2006, and November 2006 through January 2007. Yellow perch were the primary fish impinged during this period and this species was much less abundant in 1978-1982. Therefore the temporal variability in impingement is strongly influenced by the species composition in the nearfield area and the year-class strength of the dominant species.

The nearfield sampling of ichthyoplankton and fish provides a characterization of the fish community in the vicinity of Cook Nuclear Plant, and provides data for preliminary evaluation of alternate intake locations. There was general trend of decreasing density of ichthyoplankton with distance offshore, especially in 2006 when more months were sampled (Table.4-4). Alewife eggs, and post yolk-sac larvae alewife and Cyprinidae were the dominant lifestages and taxa at the nearshore station. With distance offshore, yellow perch and round goby larvae becamemore abundant. These data indicate that shoreline bulkhead intake might result in substantially higher entrainment levels than the present intake location.

The situation is less clear for adult fish. CPUE in the trawl was highest at the intake station (22 ft) followed by the experimental (40 ft) and shoreline (5 ft) stations (Figure 3-11). CPUE in the gill net was also highest at the intake station (Figure 3-10). It is not clear if fish abundance is actually higher at the 22-ft contour of the intake station, or if the intake structure is acting as an artificial reef and attracting fish. This section of Lake Michigan is relatively featureless, and any structure may attract fish, and possibly fish larvae. If this is the case, than an intake structure in almost any depth will likely attract fish.

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316(b) PHASE IIBASELINE FISH E & I STUDY Table 4-4. Mean Monthly Total Ichthyoplankton Density at Sampling Stations in the Vicinity of Cook Nuclear Plant June through November 2005 and April through November 2006 Mean Monthly Total Ichthyoplankton Density (No./ 100 M3)

Station 2005 (June-November) 2006 (April through November)

Shoreline 3.382 11.097 Intake- Surface 1.868 1.890 Intake - 11 ft 0.180 3.432 Intake - 22 ft 1.434 1.574 Experimental 20 ft 1.707 0.772 Experimental 40 ft 2.116 0.347 The results from this study and the previous UM study illustrate the variability in impingement and entrainment at Cook Nuclear Plant. Impingement and entrainment estimates are driven by the composition of the ichthyoplankton and fish community in the withdrawal waters.

Changes in the fish community of Lake Michigan such as the introduction of round goby, or the occurrence of a dominant year class ofyellow perch, are reflected.in the impingement and entrainment estimates.

5.0 LITERATURE CITED AEPS Co. (American Electric Power Service Corporation) 2005. Proposal for Information Collection Prepared for the Donald C. Cook Nuclear Plant to fulfill requirements of 40 CFR Part 125.959(b)(1). Prepared by the Environmental Services Division, Water and Ecological Resources Section, 1 Riverside Plaza, Columbus OH 43215-2373.

American Society for Quality Control. 1993. Sampling procedures and tables for inspection by attributes. ANSI/ASQC Z1.4-1993.

Bimber, D. L., M. Perrone, L.S. Noguchi and D.J. Jude. (1984) Field Distribution and Entrainment of Fish Larvae and Eggs at the Donald C. Cook Nuclear Power Plant, Southeastern Lake Mighigan, 1973-1979. Special Report No. 105 of the Great Lakes Research Division, University of Michigan. I Bunnell, D.B., C.P. Madenjian, J.D. Holuszko, T.J. Desorcie, and J.V. Adams. 2007. Status and Trends of Prey Fish Populations in Lake Michigan, 2006. U.S. Geological Survey, Great Lakes Science Center, 1251 Green Road, Ann Arbor Michigan.

Miller, R.R. 1957. Origin and dispersal of the alewife, Alosapseudoharengus,and the gizzard shad, Dorosomacepedianum, in the Great Lakes. Transactions of the American Fisheries Society 86(l):97-111.

NAI (Normandeau Associates Inc.) 2007. Cook Nuclear Plant 2005-2006 Impingement and Entrainment Study Quality Assurance Plan. Prepared for American Electric Power Service Corporation, Environmental Services, Water and Ecological Resources Section, Columbus Ohio.

UM (University of Michigan) 1986. Southeastern Nearshore take Michigan: Impact of the Donald C. Cook Nuclear Plant. The University of Michigan, Great Lakes Research Division, Publication 22.

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3,16(b) PHASE II BASELINE FISH E & I STUDY APPENDIX 20452 Cook 316b Baseline Final.doc 10/4/07 NormandeauAssociates, Inc.

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0 K) Appendix Table A. Estimated Total Number (in millions) of fish eggs, larvae, young-of-the-year and older fish Entrained by Month at 0 Cook Nuclear Plant Assuming Design Cooling Water Flow, June 2005 through February 2007 (June 2005 0

0) represents the entrainment estimate only for the last week in June.

C.

Cn Jun- Jul-, Aug- Sep- Oct- Nov- Dec- Jan- Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb.06-05 05 05 05 05 05 05 06 06 06 06 06 06 06 06 06 06 06 06 07. Jan.07 I'll CD

-n Alewife 0300-0900 0900-1500 4.55 27.39 4.39 15.97 0.82 0.39 0.34 0.06 1.51 0.00 0:00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.85 2.34 .

2.49 0.93 1.08 0.14 0.91 0.15 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 7.33 3.57 to 03 1500-2100 1.40 11.91 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.31 1.15 0.05 0.00 0.00 0.00 0.00 0.00 2.51

-S 2100-0300 5.12 23.95 0.55 0.13 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 4.07 1.66 0.21 0.85 0.00 0.00 0.00 0.00 6.79 0

0 Total 15.46 79.23 1.76 0.53 1.51 0.00 0.00 0.00 0.00 0.00 0.00 0.00 10.57 6.23 1.48 1.91 0.00 0.00 0.00 0.00 20.19 rn I-0 Common 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 carp 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.44 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.44 fin 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.58 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.58 Cyprinidae 0300-0900 0.55 2.62 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.63 5.96 4.55 0.20 0.00 0.00 0.00 0.00 0.00 12.35 PC' 0900-1500 0.00 1.32 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.82 0.25 0.00 0.00 0.00 0.00 0.00 0.00 1.11 CO) 1500-2100 0.00 0.58 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.31 0.19 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.65 2100-0300 0.11 3.52 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.98 3.53 2.66 0.00 0.00 0.00 0.00 0.00 0.00 7.17 Total 1.26 8.04 0.001 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.96 10.51 7.61 0.20 0.00 0.00 0.00 0.00 0.00 21.29 Rainbow 0300-0900 0.00 0.00 2.03 1.20 0.37 0.00 0.00 0.65 0.51 0.00 0.00 0.00 0.00 0.00 1.74 2.46 0.00 0.00 0.00 0.00 4.70 smelt 0900-1500 0.00 0.00 0.00 0.00 0.49 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.14 1500-2100 0.00 0.00 0.53 0.26 0.24 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.00 0.00 0.00 0.00 0.00 1.00 2100-0300 0.00 0.00 16.45 8.28 0.78 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.09 2.05 1.78 0.00 0.00 0.00 0.00 3.92 Total 0.00 0.00 19.01 9.74 1.87 0.00 0.00 0.65 0.51 0.00 0.00 0.00 0.00 0.09 3.93 5.24 0.00 0.00 0.00 0.00 9.77 Round goby 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.10 0.35 0.71 4.97 0.61 0.33 0.00 0.00 0.00 0.00 7.11 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.15 0.00 0.00 0.00 0.00 0.00 0.00 0.15 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.37 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.48 0

2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.39 0.28 9.02 22.49 3.62 1.57 0.00 0.00 0.00 0.00 37.37 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.41 0.49 0.64 9.73 27.68 4.23 1.90 0.00 0.00 0.00 0.00 45.11 Round 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CD whitefish 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 a) 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 . 0.00 0.00 0.00 0.00 0.00 0.14 Co Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0;14 0 Slimy 0300-0900 1.10 2.76 1.07 0.39 0.25 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 C0 sculpin 0900-1500 0.00 0.32 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 3.55 15.27 4.84 1.65 0.38 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.27 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.27 Total 4.65 18.35 5.91 2.05 0.63 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.27 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.27 (continued)

C) Appendix Table A. (Continued) 0 Spottail 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0

shiner 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00.

.R CO, 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.12 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.26 CD 2100-0300 0.00 0.00 0.13 0.06 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.29 0.58 0.87 lh CD Total 0.00 0.00 0.13 0.06 0.00 0.00 0.00 0.00 0.00 0.00 0.12 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.29 0.58 1.13 N,q Unidentified 0300-0900 0.00 0.00 0.27 0.14 0.00 0.00 0.00 0.00 0.00 0.04 0.10 0.00 0.02 0.05 0.00 0.00 0.00 0.00 0.00 0.00 0.21 CO) 0, 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.11 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.19 III C) 1500-2100 0.00 0.30 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.16 3.38 0.14 0.00 0.00 0.00 0.00 0.00 0.00 3.68 4C 2100-0300 0.00 0.26 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.09 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 Total 0.00 0.56 0.27 0.14 0.00 0.00 0.00 0.00 0.00 0.08 0.19 0.24 3.50 0.19 0.00 0.00 0.00 -0.00 0.00 0.00 4.21 CO)

White 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 sucker 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 In Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 0.00 0.13 yellow 0300-0900 0.00 0.15 0.00 0.40 0.13 1.60 2.42 0.13 0.00 0.00 0.00 0.00 0.00 0.02 0.15 0.11 0.00 0.00 0.00 0.00 0.28 perch 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.30 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.30 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.37 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.15 0.46 0.00 1.09 2100-0300 0.00 0.00 0.13 0.19 0.06 0.32 0.49 0.03 0.00 0.00 0.00 0.00 0.00 0.07 0.14 0.00 0.00 0.00 0.00 0.00 0.21 Total 0.00 0.15 0.13 0.59 0.20 1.92 2.91 0.16 0.04 0.37 0.00 0.00 0.30 0.09 0.36 0.11 0.00 0.15 0.46 0.00 1.88 All species 0300-0900 6.20 32.91 4.18 2.46 2.27 1.60 2.42 0.78 0.51 0.08 0.20 1.99 9.69 12.08 3.79 3.81 0.00 0.00 0.00 0.00 32.14 0900-1500 4.39 17.62 0.39 0.06 0.49 0.00 0.00 0.00 0.00 0.00 0.00 0.13 3.57 1.33 0.28 0.15 0.00 0.00 0.00 0.00 5.46 1500-2100 1.40 12.79 0.53 0.26 0.24 0.00 0.00 0.00 0.08 0.75 0.12 0.60 6.32 1.51 0.12 1.00 0.00 0.15 0.46 0.00 11.11 2100-0300 9.38 43.00 22.10 10.32 1.22 0.32 0.49 0.03 0.00 0.04 0.62 1.39 16.90 26.98 6.02 4.20 0.00 0.00 0.29 0.58 57.01 I Total 21.37 106.32 27.20 13.11 4.21 1.92 2.91 0.81 0.59 0.86 0.95 4.11 36.47 41.90 10.20 9.16 0.00 0.15 0.75 0.58 105.72 0

-9

4 Appendix Table B. Estimated Total Number (in millions) of fish eggs, Entrained at Cook Nuclear Plant by Month Assuming Design 4n 0 Cooling Water Flow, June 2005 through February 2007 (June 2005 represents the entrainment estimate only for the last week in June.

0 Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct7 Nov- Dec- Jan- Feb.06- CO C Species Diel 05 05 05 05 05 05 05 06 06 06 06 06 06 06 06 06 06 06 06 07 Jan.07 S' Alewife 0300-0900 4.27 15.90 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.24 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.24

. 0900-1500 4.23 6.92 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.87 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.87 1500-2100 0.70 6.98 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.29 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.29 2100-0300 4.69 13.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3.61 0.07 0.00 0.00 0.00 0.00 0.00 0.00 3.68 0

o Total 13.89 42.81 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 7.01 0.07 0.00 0.00 0.00 0.00 0.00 0.00 7.08 m Common carp 0300-0900 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 In 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.44 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.44 zO 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 4

Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.58 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.58 I0 Cyprinidae 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00' 0.00 0.00 0.00 0.69 1.57 0.15 0.00 0.00 0.00 0.00 0.00 0.00 2.40 58 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.48 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.53 44 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.31 0.12 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.50 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.28 0.70 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.98 Total 0.00 0.00 0.00 0.00 0.00 -0.00 0.00 0.00 0.00 0.00 0.00 1.32 2.88 0.22 0.00 0.00 0.00 0.00 0.00 0.00 4.42 Rainbow 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rismelt 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00' 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Round 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 goby 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00. 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2 y 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 2100-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Roud 0300-50900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rwies 0900-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 A 1500-000 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 000-0900 Tol 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

. Sloim 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 lin 0900-2100 15 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00. 0.00 0.00 0.00 0.00 0.00 0.00 0.00

. ' (continued)

Appendix Table B. (Continued) o Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb- Mar- Apr- MaY- Jun- Jul- Aog Sep- Oct- Nov- Dec- Jan- Feb.06-o Species Diel 05 05 05 05 05 06 06 06 06 06 06 06 06 06 06 06 06 07 Jan.07 Spottail 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 shine 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 a shiner 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

" 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 S 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2!Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 o Unidenti-fied U 0300-0900 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0..00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.00 0.10 0.00 0.00 0.08 0.00 0.01 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 tCO 0.00 0.00 0.00 0.00 0.00 0.194 f500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.11 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.19 o 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.16 3.16 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3.32 00.00 sk 2100-0300 0.00 0.26 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.09 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 Total 0.00 0.41 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.19 0.24 3.27 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3.78 00 White 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 sucker 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.000[

1500-2100 0.00 0.00- 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2Y100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00. 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Yellow 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 perch 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 41500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 000 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total sc0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Allspecies 0300-0900 4.27 15.90 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.10 0.69 2.95 0.15 0.00 0.00 0.00 0.00 0.00 0.00 3.92 0900-1500 4.23 6.92 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 2.45 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2.58 1500-2100 0.70 7.12 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.47 5.02 0.07 0.00 0.00 0.00 0.00 0.00 0.00 5.56 2100-0300 4.69 13.27 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.09 0.28 4.31 0.07 0.00 0.00 0.00 0.00 0.00 0.00 4.79 Total 13.89 43.22 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.19 1.56 14.74 0.29 0.00 0.00 0.00 0.00 0.00 0.00 16.86 0

(0

0Cn Appendix Table C. Estimated Total Number (in millions) of Undetermined Larval Lifestage Entrained at Cook Nuclear Plant by K) 0 4.. Month Assuming Design Cooling Water Flow, June 2005 through February 2007 (June 2005 represents the CO) 01 entrainment estimate only for the last week in June.

CD,

.03 Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb.06-0 Species Diel 05 05 05 05 05 05 05 06 06 06 06 06 06 06 06 06 06 06 06 07 Jan.07 0, Alewife 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.15 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.15 C,,

0900-1500 0.00 0:00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 C) 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 4D 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.15 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.15 Common 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 carp 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 I-0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 PC, Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Cypri- 0300-0900 0.00 0.88 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.26 1.66 0.20 0.00 0.00 0.00 0.00 0.00 3.13 nidae 0900-1500 0.00 0.45 0.00 0.00 0.00 0.00 0.00 0.00. 0.00 0.00 .0.00 0.00 0.15 0.08 0.00 0.00 0.00 0.00 0.00 0.00 0.22 C/,

1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 2100-0300 0.00 1.89 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 0.74 0.85 0.00 0.00 0.00 0.00 0.00 0.00 1.72 Total 0.00 3.22 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 2.22 2.59 0.20 0.00 0.00 0.00 0.00 0.00 5.15 Rainbow 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 smelt 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0;00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Round 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 goby 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 '0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.001 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Round 000-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 whitefish 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

.9 0 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Slimy 0300-0900 0.00 0.43 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 sculpin 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 1 0.00 0.43 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00, 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 (continued)

N)

C)

Appendix Table C. (Continued) 21 Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb.06-Species Diel 05 05 05 05 05 05 05 06 06 06 06 ' 06 06 06 06 06 06 06 06 07 Jan.07 Spottail 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 shiner 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 C-.

"13 en 0~

2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CD Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 fli

5. 0.00 0.00 0.00 0.00" 0.00 0.00 4

CD Unidentif 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.02 0.05 0.00 0.00 0.00 0.00 0.00 0.00 0.07 ied 0900-1500 0.00 CO 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.16 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.22 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.36 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.16 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.24 0.19 0.00 0.00 0.00 0.00 0.00 0.00 0.43 rrn White 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 sucker 0900-1500 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 . 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Co 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Yellow 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 -I perch 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00. 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0:00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 All 0300-0900 0.00 1.31 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.43 1.72 0.20 0.00 0.00 0.00 0.00 0.00 3.35 species 0900-1500 0.00 0.45 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.15 0.08 0.00 0.00 0.00 0.00 0.00 0.00 0.22 1500-2100 0.00 0.16 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.28 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.43 2100-0300 0.00 1.89 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 0.74 0.85 0.00 0.00 0.00 0.00 0.00 0.00 1.72 Total 0.00 3.81 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 2160 2.79 0.20 0.00 0.00 0.00 0.00 0.00 5.73 4) 0.

0) a)

P~

g Appendix Table D. Estimated Total Number (in millions) of Yolk-sac Larvae Entrained at Cook Nuclear Plant by Month Assuming C-0 Design Cooling Water Flow, June 2005 through February 2007 (June 2005 represents the entrainment estimate only 0

0 for the last week in June.

U. Jun- Jul- Aug- Sep- Oct- Nov- Dec- [qFeb] Mar Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb.06- C,,

CD Species Diel 05 05 05105105105 05 06 06 06 06 06 06 06 06 j 06 06 07 Jan.07

-I Alewife 0300-0900 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 a

CD 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

• 0.

0_

1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 rn 0 Total 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0

Common 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 carp 0900-1500 0.60 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 "1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0:00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 I-Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 0.00 0.00 0.00 Cypri- 0300-0900 0.41 1.31 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.81 1.26 1.13 0.00 0.00 0.00 0.00 0.00 0.00 3.20 nidae 0900-1500 0.00 0.74 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.11 0.10 0.00 0.00 0.00 0.00 0.00 0.00 0.22 1500-2100 0.00 0.44 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.07 2100-0300 0.43 1.10 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.56 1.16 0.77 0.00 0.00 0.00 0.00 0.00 0.00 2.49 Total 0.84 3.59 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.37 2.53 2.08 0.00 0.00 0.00 0.00 0.00 0.00 5.97 Rainbow 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 smelt 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 "0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Round 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 goby 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0,00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total . 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Round 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 whitefish 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 , 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14

0. Slimy 0300-0900 0.28 0.15 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 sculpin 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.15 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.28 0.30 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 (continued)

Appendix Table D. (Continued)

C, Jun- Jul- -Sep- Oct- Nov- Dec- Jan- Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb.06-

0) Species Diel 05 05 05 05 05 05 05 06 06 06 06 06 06 06106 06 06 06 06 07 Jan.07 0 Spottail 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 shiner 0900-1500 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00  ::-.

0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

-n CO)

Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CD Unidentif 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 ied 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 In-0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 . 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00. 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 hin 0.00 0.00 0.00 0.00 0.00 0.00 White 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 sucker 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Yellow C,

0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 perch 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.23 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.23 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 '0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.23 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.23 All 0300-0900 0.83 1.46 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.81 1.26 1.13 0.00 0.00 0.00 0.00 0.00 0.00 3.20 species 0900-1500 0.00 0.74 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.34 0.10 0.00 0.00 0.00 0.00 0.00 0.00 0.44 1500-2100 0.00 0.44 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.14 2100-0300 0.43 1.24 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.56 1.16 0.77 0.00 0.00 0.00 0.00 0.00 0.00 2.63.

Total 1.25 3.88 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 1.37 2.75 2.15 0.00 0.00 0.00 0.00 0.00 0.00 6.41.

80 0

CD

C4)

C)

.R~ Appendix Table E. Estimated Total Number (in millions) of Post Yolk-Sac Larvae Entrained at Cook Nuclear Plant by Month 0 Assuming Design Cooling Water Flow, June 2005 through February 2007 (June 2005 represents the entrainment 0

0 estimate only for the last week in June. X lo CD, Jun Jul- Aug- Sep1 Oct- Nov- Dec- Jan- Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb.06-Species Diel 05 05 005 05 05 06 06 06106106106 06106106106 06 06 07 Jan.07 ni

0) Alewife 0300-0900 0.14 11.36 0.68 0.27 1.51 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.46 2.49 0.63 0.91 0.00 '0.00 0.00 0.00 5.50 0900-1500 0.16 8.90 0.39 0.06 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.33 0.93 0.07 0.15 0.00 0.00 0.00 0.00 1.48 1500-2100 0.70 "4.93 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.02 1.07 0.05 0.00 0.00 0.00 0.00 0.00 2.14 Cd) 2100-0300 0.43 10.94 0.30 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.46 1.59 0.14 0.85 0.00 0.00 0.00 0.00 3.04 P-Total 1.43 36.13 1.37 0.33 1.51 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3.27 6.09 0.89 1.91 0.00 0.00 0.00 0.00 12.16 rn.rli Common 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 carp 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Co 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 go Cyprinidae 0300-0900 0.14 0.42 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 1.87 1.61 0.00 0.00 0.00 0.00 0.00 0.00 3.62 CO) 0900-1500 0.00 0.13 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.15 -l 1500-2100 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 -0.00 0.00 0.00 0.00 2100-0300 0.28 0.53 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0:94 1.04 0.00 0.00 0.00 0.00 0.00 0.00 1.98 Total 0.42 1.22 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 2.88 2.73 0.00 0.00 0.00 0.00 0.00 0.00 5.75 Rainbow 0300-0900 0.00 0.00 2.03 1.20 0.37 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.74 2.46 0.00 0.00 0.00 0.00 4.20 smelt 0900-1500 0.00 0.00 0.00 0.00 0.49 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.14 1500-2100 0.00 0.00 0.53 0.26 0;24 0.00 0.00 0.00 0.00 0.00 0.00 , 0.00 0.00 0.00 0.00 1.00 0.00 0.00 0.00 0.00 1.00 2100-0300 0.00 0.00 16.45 8.28 0.78 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.09 2.05 1.78 0.00 0.00 0.00 0.00 3.92 Total 0.00 0.00 19.01 9.74 1.87 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.09 3.93 5.24 0.00 0.00 0.00 0.00 9.26 Round 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.35 0.71 4.97 0.61 0.33 0.00 0.00 0.00 0.00 6.97 goby 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.15 0.00 0.00 0.00 0.00 0.00 0.00 0.15 0 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.07 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.28 9.02 22.49 3.48 1.57 0.00 0.00 0.00 0.00 36.84 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.64 9.73 27.68 4.09 1.90 0.00 0.00 0.00 0.00 44.04 Round 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CD whitefish 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 Slimy 0300-0900 0.83 2.18 0.93 . 0.39 0.25 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CD sculpin 0900-1500 0.00 0.32 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 3.55 15.12 4.84 1.46 0.32 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.27 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.27 Total 4.38 17.62 5.77 1.86 0.57 0.00 0.00 0.00 0.00 0.00 0:00 0.00 0.27 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.27 (continued)

Appendix Table E. (Continued)

DJun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb- Mar- Apr- May- Jun- Jul- Aug-] Sep- Oct- Nov- Dee- Jan- Feb.06- CAn Species Diel 05 05 05 05 05 05 05 06 06106 06 06106 06 06106 06 06 06 07 Jan.07 Spottail 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 -0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 shiner 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CO) 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Unidenti- 0300-0900 0.00 0.00 0.27 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 fled 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 rn 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.27 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 White 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 fl I.<

sucker 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 -0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 90 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.13 -I Yellow 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 perch 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 - 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 All species 0300-0900 1.10 13.96 3.91 2.00 2.13 0.00 0.00 0.00 0.00 0.00 0.00 0.49 4.04 9.07 2.98 3.70 0.00 0.00 0.00 0.00 20.28 0900-1500 0.16 9.35 0.39 0.06 0.49 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.48 1.15 0.21 0.15 0.00 0.00 0.00 0.00 1.99 1500-2100 0.70 5.08 0.53 0.26 0.24 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.02 1.15 0.05 1.00 0.00 0.00 0.00 0.00 3.22 2100-0300 4.26 26.59 21.59 9.75 1.09 0.00 0.00 0.00 0.00 0.00 0.00 0.42 10.69 25.22 5.67 4.20 0.00 0.00 0.00 0.00 46.19 I Total 6.23 54.98 26.42 12.07 3.95 0.00 0.00 0.00 0.00 0.00 0.00 0.91 16.23 36.59 8.91 9.05 0.00 0.00 0.00 0.00 71.68

D Appendix Table F. Estimated Total Number (in millions) of Young-of-the-Year Fish Entrained at Cook Nuclear Plant by Month 0 Assuming Design Cooling Water Flow, June 2005 through February 2007 (June 2005 represents the entrainment C..

0 0 estimate only for the last week in June.

co~

Jun- I Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb.06-Species Diel 05 05 05 05.05 05 05 06 06 06 06 06 06 06 06 06 06 06 06 07 Jan.07 CD CO CD Alewife 0300-0900 0.00 0.13 0.14 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.45 0.00 0.00 0.00 0.00 0.00 0.45 5" 0900-1500 0.00 0.15 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.07 C'n mCL 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0

2100-0300 0.00 0.00 0.25 0.13 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.07 Total 0.00 0.28 0.39 0.20 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.59 0.00 0.00 0.00 0.00 0.00 0.59 C:,

Common 0300-0900 0.00 . 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 m rn carp 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Cyprinidae 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0900-1500 0.00 0.00. 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

. Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rainbow 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 - 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 smelt 0900'1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 "0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Round 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.10 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 goby 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 1500-2100 0.00 0.00 0.00 000 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

• ~1 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.39 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.53 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.49 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.67 Round 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 whitefish 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.0.0 0.00 0.00 0.00 0.00

0) 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CII Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 C')

0 Slimy 0300-0900 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 sculpin 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0) 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 5') 2100-0300 0.00 0.00 0.00 .0.19 0.06 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.14 0.19 0.06 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 p (continued)

Appendix Table F. (Continued) 0 0

1Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb- Mar- Apr- May- Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb.06-01 0)

Species Diel 05 05 05 05 05 05 05 06 06 06 06 06 06 06 06 06 06 06106' 07 Jan.07 CD Spottail 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 6.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 shiner 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CO, 0L 0 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.13 0.06 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.29 0.58 0.87 Total 0.00 0.00 0.13 0.06 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00. 0.00 0.00 0.29 0.58 0.87 C) a Unidenti- 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 fled 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 0.00 C,,

rn1 White 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 0.00 sucker 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 ,0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Yellow 0300-0900 0.00 0.15 0.00 0.20 0.07 1.60 2.42 0.13 0.00 0.00 0.00 0.00 0.00 0.02 0.15 0.11 0.00 0.00 0.00 0.00 0.28 perch 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.15 0.46 0.00 0.68 2100-0300 0.00 0.00 0.13 0.19 0.06 0.32 0.49 0.03 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.14 Total 0.00 0.15 0.13 0.39 0.13 1.92 2.91 0.16 0.00 0.00 0.00 0.00 0.00 0.02 0.36 0.11 0.00 0.15 0.46 0.00 1.10 All species 0300-0900 0.00 0.28 0.27 0.27 0.07 1.60 2.42 0.13 0.00 0.04 0.10 0.00 0.00 0.02 0.60 0.11 0.00 0.00 0.00 0.00 0.87 0900-1500 0.00 0.15 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.07 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.15 0.46 0.00 0.68 2100-0300 0.00 0.00 0.51 0.57 0.13 0.32 0.49 0.03 0.00 0.00 0.39 0.00 0.00 0.00 0.34 0.00 0.00 0.00 .0.29 0.58 1.61 Total 0.00 0.43 0.79 0.84 0.19 1.92 2.91 0.16 0.00 0.04 0.49 0.00 0.00 0.02 1.09 0.11 0.00 0.15 0.75 0.58 3.23 0

.0

4o Appendix Table G. Estimated Total Number (in millions) of Yearling and Older Fish Entrained at Cook Nuclear Plant by Month Assuming Design Cooling Water Flow, June 2005 through February 2007 (June 2005 represents the entrainment estimate only for the last week in June. CO)

Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb- Mar- Apr- May- Jun- Jul- Aug-Species Diel 05 05 05 05 05 05 06 06 06 Sep Oct-Nov- Dec- fa n- Feb.06-C.) 06 06 06 007 Jan.07 Alewife 0300-0900 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 CD 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CO) 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 '0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 Prr Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 I'l Common 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 carp 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CIo Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Cyprini- 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 dae 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rainbow 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.65 0.51 0.00 0.00 0.00 0.00 0.00 0.00 .0.00 0.00 0.00 0.00 0.00 0.51 smelt 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.65 0.51 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.51 Round 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 goby 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.37 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.41 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.37 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.41 Round 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.

whitefish 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Co Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Co 0 Slimy 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 sculpin 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

-I. 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CD

.50 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 1 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 p (continued)

Appendix Table G. (Continued) 0 01 0

Jun- Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb- Mar- Apr- May- Jun-Jul- Aug- Sep- Oct- Nov- Dec- Jan- Feb.06-CO~

Species Diel 05 05 05 05 05 05 05 06 06 06 06 06 06 06 06 06 06 06 06 07 Jan.07 Spottail 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 shiner 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.12 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.26 CD 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.60 0.00 0.00 CO)

Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.12 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.26 (1)

C Unidenti- 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0 fled 0900-1500 0.00 0.60 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 rn.

4, 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00. 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 White 0300-0900 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 sucker 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 CO) 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Total 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Yellow 0300-0900 0.00 0.00 0.00 0.20 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 perch. 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.04 0.37 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.41 2100-0300 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.07 Total 0;00 0.00 0.00 0.20 0.07 0.00 0.00 0.00 0.04 0.37 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.48 All 0300-0900 0.00 0.00 0.00 0.20 0.07 0.00 0.00 0.65 0.51 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.51 species 0900-1500 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.14 1500-2100 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.75 0.12 0.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1.09 2100-0300 0.00 0.00 0.00 ' 0.00 0.00 0.00 0:00 0.00 0.00 0.00 0.00 0.00 0.00 0.07 0.00 0.00 0.00 0.00 0.00 0.00 0.07 Total 0.00 0.00 0.00 0.20 0.07 0.00 0.00 0.65 0.59 '0.75 0.12 0.14 0.14 0.07 0.00 0.00 0.00 0.00 0.00 0.00 1.81 0

a-

1 Appendix Table H. Estimated Number of Fish Impinged at Cook Nuclear Plant Unit 1, Assuming Design Cooling Water Flow, June 2005 through January 2007 (June 2005 represents the impingement estimate only for the last week in June).

0 0

2005 2006 2007 CD, co S' Species Diel Jun I Jul Aug Sep[Oct Nov Dec Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Jan Jan.07 CD Alewife 0600-1800 26 0 28 392 144 0 0 65 147 14 53 374 30,895 331 274 4 0 1,386 478 42 33,997

h. 1800-0600 21 7 7 217 59 0 105 46 46 15 11 641 3,275 261 424 14 0 1,965 1,014 150 7,815 Total 47 7 35 609 204 0 105 111 193 29 63 1,015 34,170 592 698 18 0 3,351 1,492 192 41,812 CO)

C,n CD 9 Bloater 0600-1800 0 0 28 3,031 143 0 175 56 14 44 56 0 0 0 0 0 0 0 125 11 249 5- rn 1800-0600 0 0 26 637 233 0 196 21 0 0 4 0 0 0 0 15 0 -14 94 0 126 Total 0 0 54 3,668 376 0 3 71 77 14 44 60 0 0 0 0 15 0 14 219 11 375 Bluegill 0600-1800 0 0 0 0 0 0 0 28 0 152 0 0 0 0 0 0 0 0 28 0 179 1800-0600- 0 0 0 0 0 0 0 4 7 66 25 0 0 0 0 1 10 0 39 21 168 Total 0 0 0 0 0 0 0 32 7 218 25 0 0 0 0 1 10 0 67 21 347 C'n Bluntnose 0600-1800 0 0 0 0 0 0 0. 0 0 0 0 0 4 0 0 0 0 0 0 0 4 minnow 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

.O)

Total 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 .0 0 0 0 0 4 -ft Brook 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0 0 0 0 0 silverside 1800-0600 0 0 0 0 0 0 0 0 0 14 0 0 0 0 0 0 0 0 0 0 14 Total 0 0 0 0 0 0 0 0 0 14 0 0 0 0 0 0 0 0 0 0 14 Brown 0600-1800 0 0 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 bullhead 1800-0600 0 0 0 0 0 0 0 7 0 11 4 0 0 0 0 0 0 0 0 0 15 Total 0 0 12 0 0 0 0 7 0 11 4 .0 0 0 0 0 0 0 0 0 15 Brown trout 0600-1800 0 0 0 0 0 0 0 0 0 20 0 0 3 72 0 0 0 0 0 0 96 1800-0600 0 7 0 0 0 0 0 0 0 0 4 4 0 21 0 0 0 0 0 0 29 Total 0 7 0 0 0 0 0 0 0 20 4 4 3 93 0 0 0 0 0 0 124 Burbot 0600-1800 7 0 0 0 0 0 0 28 0 18 0 14 4 0 0 0 0 3 14 4 56 Z 1800-0600 0 0 0 0 7 0 0 0 21 81 7 4 3 0 0 0 0 14 8 28 166 Total 7 0 0 0 7 0 0 28 21 98 7 18 7 0 0 0 0 17 22 31 222 Central 0600-1800 0 0 0 0 0 0 0 0 0 34 0 0 0 0 0 0 0 .0 0 0 34 mud- 1800-0600 0 0 0 0 0 0 0 70 -0 .14 4 0 0 0 0 0 0 0 0 0 18 minnow Total 0 0 -0 0 0 0 0 70 0 48 4 0ý- 0 0 0 0 - 0 0 0 52 Channel 0600-1800 0 0 0 7 0 0 0 . 4 130 94 11 4 0 0 14 0 0 -0 23 56 331 catfish 1800-0600 0 0 0 0 8 140 28 0 340 221 53 11 11 0 4 1 10 15 8 45 717

_Total 0 0 0 7 8 140 28 4 469 315 64 14 11 0 18 1 10 15 31 101 .1,048 01 Chestnut 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 CD) lamprey 1800-0600 0 0 0 0 0 0 0 0 -0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Chinook 0600-1800 0 0 0 0 0 0 0 0 0 28 0 0 0 0 0 0 0 0 0 0 28 1800-0600 0 0 0 .0 0 0 0 0 0 70 21 0 0 0 0 0 0 0 0 0 91 Total 0 0 0 0 0 0 0 0 0 98 21 0 0 0 0 0 0 0 0 0 119 (continued)

PI) Appendix Table H. (Continued) 0 CD 2005 2006 2007

~Feb.06-Species Diel Jun Jul Aug Sep Oct Nov Dec Jan Feb I Mar Apr May Jun Jul Aug Sep Oct Nov Dec Jan Jan.07

-n Coho 0600-1800 0 0 0 0 0 0 0 0 0 20 14 0 0 0 0 0 0 0 0 0 34 CO) 0C>

0 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0. 0 0 0 4D Total 0 0 0 0 0 0 0 0 0 20 14 0 0 0 0 0 0 0 0 0 34 Common 0600-1800 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 4 carp 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 14 0 18 CO)

CD 0o Total 0 0 0 0 0 0 0 0 0 4t 0 0 3 0 0 0 0 0 14 0 21 0x Deepwater 0600-1800 0 0 0 0 0 0 0 20 0 0 20 w

-0

.n sculpin 1800-0600 0 0 0 0 0 0 0 0 0 14 4 0 0 0 0 0 0 0 0 0 18 Total 0 0 0 0 0 0 0 0 0 14 4 0 0 0 0 0 0 20 0 0 38 Eastern 0600-1800 0 0 0 0 0 0 0 0 0 34 0 0 0 0 0 0 0 0 0 0 34 banded 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 rrn killifish Total 0 0 0 0 0 0 0 0 0 34 0 0 0 0 0 0 0 0 0 0 34 Flathead 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 .0 0 0 0 CO) catfish 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 .0 Total 0 0 ý0 0 0 .0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Freshwater 0600-1800 0 0 7 0 0 0 0 0 0 0 0 4 0 0 4 0 0 0 0 0 7 drum 1800-0600 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 6 4 0 8 0 26 Total 0 0 7 0 0 0 0 0 0 0 0 11 0 0 4 6 4 0 8 0 33 Gizzard 0600-1800 0 0 14 0 138 910 840 175 206 510 0 0 0 0 0 4 58 229 2,186 378 3,571 shad 1800-0600 0 0 13 15,890 362 700 175 224 175 575 21 0 0 0 0 1 10 252 2,870

• 752 4,656 Total 0 0 27 15,890 500 1,610 1,015 399 381 1,085 21 0 0 0 0 5 68 481 5,056 1,130 8,227 Golden 0600-1800 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 15 22 redhorse 1800-0600 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 14 0 18 Total 0 0 0 0 0 0 0 0 0 0 0 11 0 0 0 0 0 0 14 15 40 0 Golden 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 shiner 1800-0600 0 0 0 0 0 0 0 .0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 "0 0 0 0 0.0 .0 0 0 0 0 0 0 *0 0 0 0 Greater 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 7 0R redhorse 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 7 13 Lakechub 0600-1800 0.0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Lake 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14 0 14 sturgeon 1800-0600 0 *0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14 0 14 Lake trout 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 11 0 0 0 0 3 ,3 0 17 1800-0600 0 0 0 0 0 0 0 4 0 4 0 4 3 0 0 0 0 0 25 0 36 Total 0 0 0 0 0 0 0 4 0 4 0 4 14 0 0 0 0 3 29 0 53 (continued)

Appendix Table H. (Continued) ci, 0

0 2005 2006 2007 0

eueFeb.06-Species Diel Jun Jul Aug Sep Oct Nov Dec Jan Feb Mar Apr May Jun Jul Aug Sep Oct I Nov Dec Jan Jan.07 Lake 0600-1800 0 7 0 0 0 0 315 51 156 105 14 0 0 14 0 0 0 24 1,560 180 2,053 co C.' whitefish 1800-0600 0 0 0 0 0 140 168 168 557 33 42 7 0 0 0 -0 0 4 1,155 585 2,383 0) 3" C- Total 0 7 0 0 0 140 483 219 713 137 56 7 0 14 0 0 0 28 2,715 765 4,436 0~

Largemouth 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 to bass 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Longnose 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 "0 0 0 C07 dace 1800-0600 '0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Zl.

Longnose 0600-1800 0 0 0 0 0 0 0 28 0 144 .32 25 18 7 4 0 0 0 35 36 299 sucker 1800-0600 7 0 0 70 0 0 0 0 18 73 42 11 3 *0 0 0 0 0 21 24 193 MI Total 7 0 0 70 0 0 0 28 18 216 74 35 21 7 4 0 .0 01 56 61 492 Mottled 0600-1800 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 3 0 0 7 sculpin 1800-0600 0 .0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 4 Total 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 3 0 0 11 Ninespine 0600-1800 0 0 0 .0 0 0 0 0 0 0 35 70 4 0 0 0 0 0 0 0 108 stickleback 1800-0600 0 0 .0 0 0 0 0 0 14 0 7 21 7 0 0 0 0 0 0 0 49 Total 0 0 0 0 0 0 0 0 14 0 42 91 10 0 0 0 0 0 0 0 158 Northern 0600-1800 0 0 0 0 0 0 0 0 0 20 .0 0 0 0 0 0 0 0 0 0 20 pike 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 20 0 0 0 0 0 0 0 0 0 0 20 Pumpkin- 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 seed 1800-0600 0 0 0 0 0 0 0 0 4 15 0 0 0 0 0 0 0 0 0 0 18 Total 0 0 0 0 0 0 0 0 4 15 0 0 01 0 0 0 0 0 0 0 18 Rainbow 0600-1800 0 0 182 28 0 0 0 88 819 221 189 193 4 7 0 0 0 21 271 275 2,001 smelt 1800-0600 0 14 33 0 0 0 56 39 631 606 21 107 7 14 4 0 7 28 143 428 1,996 Total 0 141 215 28 0 0 56 126 1,450 827

• 210 301 11 21 4 0 7 49 415 704 3,997 Rock bass 0600-1800 0 0 0 0 0 0 0 0 0 13 0 0 0 0 0 0 0 0 0 0 13 0 1800-0600 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 7 7 Total 0 0 0 0 0 0 0 7 0 13 0 0 0 0 0 0 0 0 0 7 20 Roundgoby 0600-1800 164 241 330 21 135 0 315 305 589 371 553 3,614 971 620 314 127 8 475 775 319 8,736 1800-0600 478 198 110 630 21 1,815 91 287 394 556 729 3,034 520 336 203 78 56 313 784 189 7,193 0 Total 642 439 440 651 156 1,815 406 592 983 928 1,282 6,648 1,491 . 956 517 205 64 788 1,559 508 15,929 Sealamprey 0600-1800 0 0 0 . 0 0 0 0 0 189 24 4 4 .0 0 0 0 0 0 0 46 266 1800-0600 0 0 0 0 0 0 0 11 11 54 21 0 0 0 .0 0 0 0 0 24 110 Total 0 0 0 0 0 0 0 11 200 78 25 4 0 0 0 0 0 0 0 70 376 Shorthead 0600-1800 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 redhorse 1800-0600 7 0 0 70 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 4 Total 7 0 7 70 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 4 (continued)

Appendix Table H. (Continued) 0 0

0, Species Diel Jun Jul Aug 2005 Sep Oct Nov I r II Dec Jan Feb Mar Apr May 2006 _

Jun Jul Aug ISep Oct Nov Dec Jan "2007 b.06 Jan.07 Silver 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 - 0 0 0 0 0 0 rn redhorse 1800-0600 -0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0~ Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 a Slimy 0600-1800 0 0 0 0 0 0 0 0 70 66 98 35 46 14 0 0 0 4 0 0 331 co sculpin 1800-0600 0 0 0 0 0 0 0 7 49 102 131 32 42 0 0 0 0 0 0 14 371 Total 0 0 0 0 0 0 0 7 119 168 229 67 88 14 0 0 0 4 0 14. 702 0.

Smallmouth 0600-1800 0 0 .0 0 0 0 0 0 4 0 ,0 0 0 0 0 0 0 0 0 0 4 bass 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

_Total 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 4 rn rn Spottail 0600-1800 59 53 670 308 105 910 2,590 323 3,772 877 1,350 468 102 55 45 26 15 203 13,792 960 21,665 shiner 1800-0600 119 84 73 2,800 657 1,666 2,226 1,334 3,653 2,565 1,336 187 28 43 120 47 0 448 6,883 1,375 16,685 00 Total 179 137 743 3,108 762 2,576 4,816 1,657 7,425 3,442 2,686 655 129 98 165 73 15 652 20,675 2,336 38,350 Steelhead 0600-1800 0 0 0 0 0 0 0 18 7 4 4 0 0 0 0 0 0 0 0 0 14 CO) 1800-0600 0 7 0 0 0 0 0 70 0 7 0 0 0 0 0 0 0 0 0 0 7 (I Total 0 7 0 0 0 0 0 88 7 10 4 0 0 0 0 0 0 0 0 0 21 Threespine 0600-1800 0 0 0 0 0 0 0 4 394 57 32 14 11 0 0 0 0 0 13 31 551 lb stickleback 1800-0600 7 0 0 0 0 *0 28 7 81 92 25 46 28 0 0 0 0 0 14 29 315 Total 7 0 0 0 0 0 28 11 475 150 56 60, 38 0 0 0 0 0 26 60 866 Trout-perch 0600-1800 0 0 0 0 0 0 0 28 0 20 0 14 0 0 0 0 0 ' 14 111 0 158 0 1800-0600 0 0 0 0 0 0 0 0 0 29 0 4 10 0 0 0 0 0 39 24 106 Total 0 0 0 0 0 0 0 28 0 49 0 17 10 0 0 0 0 14 150 24 265 Unidentified 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Walleye 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 "0 0 0 1800-0600 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 7 0 0 0 0 0 0 0 0 0 0 0 .0 0 0 0 0 0 0 0 0 White perch 0600-1800 0 0 0 0 0 0 0 0 35 28 0 .0 4 0 0 0 0 0 0 7 74 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14 21 35 Total 0 0 0 0 0 0 0 0 35 28 0 0 4 .0 0 0 0 0 14 28 108 White 0600-1800 7 0 0 0 0 0 0 0 4 35 0 25 11 3 0 0 0 0 14 18 108 sucker 1800-0600 7 0 7 0 0 0 0 7 0 37 4 4 21 0 0 4 0 0 14 31 113

• 0 Total 14 0 7 0 0 0 0 7 4 7i 4 28 32 3 0 4 0 0 28 48 221 Yellow 0600-1800 72 87 5,431 17,681 2,742 67.,270 10,080 20,366 69,780 28,188 11,910 5,126 1,235 322 2,110 131 42 10,044 51,292 8,956 189,136 perch 1800-0600 56 148 728 21,140 3,280 49,213 3,073 18,780 154,971 34,231 14,760 6,696 1,174 353 2,802 180 4 6,543 20,231 11,498 253,444 A) Total 128 235 6,159 38,821 6,022 116,483 13,153 39,146 224,751 62,419 26,670 11,821 2,410 675 4,912 311 46 16,586 71,524 20,454 442,580 Total 0600-1800 335 388 6,709 21,468 3,406 69,090 14,315 21,566 76,314 31,144 14,355 9,987 33,318 1,444 2,765 292 124 12,431 70,734 11,341 264,250 1800-0600 710 465 997 41,455 4,628 53,675 6,146 21,0911160,970 39,486 17,279 10,821 5,137 1,028 3,556 346 101 9,595 33,394 15,245 296,961 Total 1,045 853 7,706 62,923 8,034 122,765 20,461 42,656 237,285 70,631 31,635 20,808 38,455 2,473 6,321 638 225 22,026 104,128 26,586 561,211

Appendix Table I. Estimated Biomass (g) impinged at Cook Nuclear Plant Unit 1, Assuming Design Cooling Water Flow, June 2005 0

0 through January 2007 (June 2005 represents the biomass impingement estimate only for the last week in June). C,,

0 2005 . 2006 C. Species Diel Jun Jul Aug Sep Oct Nov Dec Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Alewife 0600-1800 413 0 91 756 906 0 0. 246 441 518 1,673 3,200 180,025 2,848 4,012 4 0 2,765 1,479 m 1800-0600 682 203 139 232 163 0 770 102 172 59 179 5,164 21,998 1,968 6,150 32 0 6,124 2,588 Total 1,095 203 230 988 1,069 0 770 348 613 577 1,852 8,365 202,023 4,816 10,162 35 0 8,889 4,068

-C, Bloater 0600-1800 0 0 56 6,454 319 0 595 700 77 171 980 0 0 0 0 0 0 0 683 M

0 1800-0600 0 0 53 1,547 519 0 3,801 133 0 0 15 0 O 0 0 58 0 70 401 Total 0 0 109 8,001 838 0 4,396 833 171 995 0 0 m

77 0 0 58 0 70 1,085 Bluegill 0600-1800 0 0 0 0 0 0 0 140. 0 318 0 0 0 0 0 0 0 0 83 m

1800-0600 0 0 0 0 0 0 0 21 28 306 50 0 0 0 0 1 20 0 306 "In Total 0 0 0 1 0 0 0 161 28 624 50, 0 0 0 0 1 20 0 389 Bluntnose 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 14 0 0 0 0 0 0 minnow 1800-0600 0 0 0 0 0 .0. 0 0 0 0 0 0 0 0 0 0 0 0 0 2C, Total -1 0 0 01 0 0 0 0 0 0 0 0 0 14 0 0 0* 0 0 0 Brook 0600-1800 0 0 0 0 0 0 0 0. 0 0 0 0 0 0 0 0 0 0 N 0

silverside 1800-0600 0 0 0 0 0 0 0 0 0 .14 0 0 0 . 0 0 0 0 0 Total 0 0 01 0 0 0 01 0 0 14 0 0 0 0. 0 0 0 0 0 Brown 0600-1800 0 0 9,588 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 bulthead 1800-0600 0 0 0 0 0 0 0 2,093 0 55 57 0 0 0 0 0 0 0 0 Total, 0 0 9,588 0 0 0 0, 2,093 0 55 57 0 0 0 0 0 0 0 0 Brown 0600-1800 0 0 0 0 0 0 0 0 0 6,149 0 0 20,367 60,420 0 0 0 0 0 trout 1800-0600 0 23,425 0 0 0 0 0 0 0 0 6,337 3,150 0 11,324 0 0 0 0 0 Total 0 23,425 0 0 0 0 0 0 0 6,149 6,337 3,150 20,367 71,744 0 0 0 0 0 Burbot 0600-1800 459 0 0" 0 0 0 0 308 0 105 0 116 133 0 0 0 0 27 4,270 1800-0600 0 0 0 0 175 01 0 0 93 391 137 60 132 0 .0 0 0 140 146 Total 459 0 0 0 175 0 0 308 93 496 137 176 *265 0 0 0 0 167 4,416 0

Central 0600-1800 0 0 0 0 0 0 0 0 0 137 0 0 0 0 0 0 0 0 0 mud- 1800-0600 0 0 0 0 0 0 0 560 0 210 29 0 0 0 0 .0 0 0 0 minnow Total 0 0 0 0 "0 0 0 560 0 347 29 0 0 0 0 0 0 0 0 Channel 0600-1800 0 0 0 322 0 0 0 8 256 326 40 25 0 0 51,1.00 0 0 0 5,930 wb catfish 1800-0600 0 0 0 0 23 560 12,824 0 1,089 749 1,810 40 5,812 0 1,617 12 167 934 259 Total 0 0 0 322 23 560 12,824 8 1,344 1,075 1,850 65 5,812 0 52,717 12 167 934 6,189 Chestnut 0600-18 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 01 lamprey 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 -0 0 0 0 0 0 0 0 0 0 0 0 0 Chinook 0600-1800 0 0 0 0 0 0 0 0 0 25,522 0 0 0 0 0 0 0 0 0 1800-0600 0 0 0 0 0 0 0 0 0 29,756 20,102, 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 01 0 01 0 55,278 20,102 0 0 0 0 0 01 0 0 (continued)

Appendix Table I. (Continued)

C) 0 0

0, Species Diel

{ Jun Jul [Aug 2005 Sep Oct Nov Dec Jan . Feb MarIApr IMay Jun 2006 Sep Nov Dec Coho 0600-1800 0 0 0 0 0 0 0 0 0 17,116 16,694 0 0 0 0 0 0 0 0 1800-0600 0 0 0 0 0 0 0 0 0 0 0 .0 0 0 0 0 0 0 0

. Total 0 0 0 0 0 0 0 0 0 17,116 16,694 0. 0 0 0 0 0 0 0 0~ Common 0600-1800 0 0 0 0 0 0 0 0 0 10,507 0 0 0 0 0 0 0 0 0 I-to carp 1800-0600 0 0 0 0 0 0 0 0 0 0 0 5,151 0 0 0 0 0 129 )h.

h~i C)

Total 0, 0 0 0 0 0 0 0 010,507 01 0 5,151 0 0 0 0 0 129 co C) 4 Deep- 0600-1800 0 0 0 0 0 0 0 .0 0 0 0 0 .0 0 0 0 0 .383 0 water 1800-0600 0 0 0 0 0 0 0 0 0 252 194 0 0 0 0 .0 0 0 0 sculpin Total 0 0 0 0 0 0 0 0 10 252 194 0 0 0 .0 0 0 383 0 rrn Eastern 0600-1800 0 0 0 0 0 0 0 0 0 82' 0 0 1 0 0 0. 0 0 0 banded 1800-0600 0 0 0 0 0 0 0 0 - 0 0 0 0 0 0 0 0 0 0 killifish Total 0 0 01 0 0 0 "0 0 0 82 0 0 0 0 0 0 0 0 0 M<

-I:

Flathead 0600-1800 0 0 .0 0 0 0 0 0 0. 0 0 0 0 0 0 0 0 0 0 catfish 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 .0 0 0 0 01 0 0 0 0 0, 0 0 0 0 .0 0 Fresh- 0600-1800 0 0 5,229 0 0 0 0 0 0 0 0 2,380 0 0 2,856 0 0 0 0 water 1800-0600 0 0 0 0 0 0 0 0 0 0 0 3,017 0 0 0 4,407 2,938 0 154 drum Total 0 0 5,229 01 0 01 0 0 0 0 01 5,397 0 0 2,856 4,407 2,938 01 154 0> Gizzard 0600-1800 0 0 91 0 808 5,180 5,460 1,376 1,272 18,379 0 0 0 .0 0 16 222 1,476 20,907 shad 1800-0600 0 0 40 51,730 346 2,380 2,275 2,735 7,036 44,939 212 0 0 0 0 6 78 4,067 20,394 Total 0 0 131 51,730 1,154 7,560 7,735 4,111 8,308 63,318 212 0 0, 0 0 21 301 5,543 41,301 Golden 0600-1800 0 0 0 0 0 0 0 0 0 0 0 9,614 0 0 0 0 0 0 0 0O redhorse 1800-0600 0 0 0 0 0 0 0 0 0 0 25 0 0 0 0- 0 0 0 2,645 Total 0 0, 0 0 0 01 0 0 0 0 0, 9,639 0 0 0 0 0 0, 2,645 Golden 0600-1800 0 .0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 shiner 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0. 0 Total 0 0 0, 0 0, 0 0 0 0 0 0] 00 0 0 0 0 0 0 0 Greater 0600-1800 0 0.. 0 0 0 0 0 0 .0. 0 0 0 0 0 0 0 0 0 .0 redhorse 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 . 0 Total 0 0j 0 0 0 01 0 0 0 0 0 0 0 0 0 0 0 0 0 CD Lake 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 chub 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Cn Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Lake 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9,212 sturgeon 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 -0 0 0 0 0 0 0 0 9,212 CD Lake 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 217 0 0 0 0 .18,686 7,486 trout 1800-0600 0 0 0 0 0 0 0 10,573 0 9,855 0 7,175 59 0 0 0 0 0 48,713 Total 0 0 0 0 0 0 0 10,573 0 10 9,855 7,175 276 0 0 0 0 18,686 56,199 (continued)

Appendix Table I. (Continued) 01 M

0 0 2005 2006 Species ' Diel Jun Jul- Aug Sep Oct Nov Dec Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec co Lake 0600-1800 0 14 .0 0 0 0 30,520 9,099 5,657 27,319 207 0 0 3,327 0 0 0 5,951 245,412 whitefish 1800-0600 0 0 0 0 0 41,580 48,468 9,394 69,565 5,639 5,613 4,735 0 0 0 0 0 3,936 115,999

-n Total 0 14 0 0 0 41,580 78,988 18,493 75,222 32,958 5,821 4,735 0 3,327 0 0 0 9,887 361,411 CD, Large- 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 mouth 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0. 0 0 0 0 0 0 0 0

.L bass Total 0 0 0 - 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 co Cl,

-n Longnose 06 0 0-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 dace 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 rri Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Longnose 0600-1800 0 0 0 0 0 0 0 20,104 0 119,953 24,938 21,970 18,907 3,955 1,155 0 0 0 26,112 sucker 1800-0600 6,291 0 0 91,000 0 0 0 0 29,750 67,868 32,409 6,004 2,812 0 0 0 0 0 ,36,954 1 Total 6,291, 0 0 91,000 0 0 0 20,104 29,750 187,821 57,348 27,974 21,719 3,955 1,155 0 0 0 63,067 Mottled 0600-1800 0 0 0 0 0 0 0 0 0 0 28 0 0 0 0 0 0 41 0 sculpin 1800-0600 0 0 0 0 0 0 0 0 0 0 40 0 0 0 co 0 0 0 0 0 C,,

Total 0 0 0 0 0 0 0 0 0 0 68 0 _0 0 0, 0 0 41 0 Ninespine 0600-1800 0 0 0 0 0 0 0 0 0 0 77 640 11 0 0 0 0 0 0 stickle- 1800-0600 0 0 0 0 0 0 0 0 35 0 18 60 14 0 0 0 0 0 0 back Total 0 01 0 01 0 0 0 0 35 0 95 700 24 0 0 0 0 0 0 Northern 0600-1800 0 0 0 0 0 0 0 0 0 363 0 0 0 0 0 0 0 pike 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 363 0 0 0 0 0 0 0 0 0 Pumpkin- 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 seed 1800-0600 0 0 0 0 0 0 0 0 130 652 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 130 652 0 0 0 0 0 0 0 0 0 Rainbow 0600-1800 0 0 1,022 336 0 0 0 98 3,948 505 483 471 7 21 0 0 0 . 62 1,875 0 smelt 1800-0600 0 56 152 0 0 0 980 368 1,495 11,703 21 111 7 14 4 0 7 84 1,223 Total 0 56 1,174 336 0 0 980 466 5,443 12,209 504 582 14 35 4 0 7 146 3,098 Rock bass 060041800 0 0 0 0 0 0 0 0 0 159 0 0 0 0 0 0 0 0 0 1800-0600 0 0 0 0 0 0 0 14 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 14 0 159 0 0 0 0 -0 0 0 0 0 C,, Round 0600-1800 1,949 2,834 4,050 210 2,300 0 3,325 4,806 4,239 3,655 8,036 58,693 8,836 6,523 3,925 1,700 424 9,802 18,303 0 goby 1800-0600 6,460 2,851 1,436 5,460 259 33,646 2,065 3,549 4,025 5,394 11,009 46,189 6,601 3,874 1,897 854 1,433 6,802 10,367 C, Total 8,409 5,685 5,486 5,670 2,559 33,646 5,390 8,355 8,264 9,049 19,044 104,882 15,437 10,398 5,821 2,554 1,857 16,604- 28,670 0

Sea 0600-1800 0 0 0 0 0 0 0 0 53,449 7,045 1,341 168 0 0 0 0 0 0, 0 0*

lamprey 1800-0600 0 *0 0 0 0 0 0 4,067 2,751 13,087 6,122 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 _ 0 0 4,067 56,200 20,132 7,462 168 0 0 0 0 0 0 0 Short- 0600-1800 0 .07,701 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

'5, head 1800-0600 5,20200 119,000 0 0 0 0 0 777 0 0 0 0 0 0 0 0 0 redhorse Total 5,202 07,701 119,000 0 0 0 . 0 0 777 0 0 0 0 0 0 0 0 0 (continued)

Appendix Table I. (Continued) 4, 0- .o 0 2005 1 2006 0

Species Diel Ju _u _u Sep Oct Nov Dec I J Feb Mar Apr May Jun Jul IAug SepIOct Nov Dec Silver, 0600-1800 0 0 01 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 redhorse 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Co C- Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Slimy 0600-1800 0 0 0 0 0 0 0 0 1,050 509 791 248 415 137 0 0 0 49 0 sculpin 1800-0600 0 0 0 0 0 0 0 126 551 905 1,002 232 324 0 0 0 0 0 0 hi 0 Total 0 0 0. 0 0 0 0 126 1,601 1,414 1,792 480 739 137 0 0, 0 49 0 04 Small- 0600-1800. 0 0 0 0 0 0 0 0 25 0 0 0 0 0 0 0 0 0 0 mouth 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 rI-bass Total *0 0 0 0 0 0 0 0 25 0 0 0 0 0 0 0 0 0 0 Spottail 0600-1800 282 327 4,817 2,576 375 4,060 27,475 2,732 31,760 7,958 9,655 4,403 995 584 345 152 144 1,074 109,271 C shiner 1800-0600 1,083 994 456 15,050 1,365 10,936 21,364 10,247 28,400 23,971 11,682 2,176 293 411 1,228 488 0 2,109 81,697 Total 1,365 1,321 5,273 17,626 1,740 14,996 48,839 12,979 60,160 31,928 21,337 6,578 1,288 995 1,572 640 144 3,183 190,968 Steelhead 0600-1800 0 0 0 0 0 0 0 1,383 7,000 497 4,841 0 0 0 0 0 0 . 0 0

.1800-0600 0 5,679 0 0 0 0 0 9,590 0 4,650 0 0 0 0 0 0 0 0 0 Total 0 5,679 1 0 0 0 0 0 10,973 7,000 5,147 4,841 0 0 0 0 .0 0 0 0 -4 Three- 0600-1800 0 0 0 0 0 0 0 4 471 78 49 17 14 0 0 0 0 0 16 spine 1800-0600 14 0 0 0 0 0 28 7 126 153 46 75 38 0 0 0 0 0 14 stickle- Total 14 0 0 0 0 0 28 11 597 230 95 92 52 0 0 0 0 0 30 back Trout- 0600-1800 0 0 0 0 0 0 0 196 0 81 0 58 0 0 03 0ý 0 70 747 perch 1800-0600 0 0 0 0 0 0 0 0 0 172 0 11 49 0 0 0 0 0 422 Total 0 0 0 0 0 0 0 196 0 253 0 69 49 0 0 0 0 70 1,169 Unidenti- 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 01 fled 1800-0600 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Wle Total 0 0 0 0 0 0 0 .0 0 0 0 0 0 0 0 0 0 0 0 Walleye 0600-1800 0 03 0 0 0 0 0 0 0 0 0 03 0 0 03 0 0 0 03 0 1800-0600 12,653 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 12,653 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 .0 0 0 White 0600-1800 0 0 0 0 0 0 0 0 1,365 5,278 0 0 2,566 0 0 0 0 0 0

0. perch 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0. .0 0 *0 0 0 0 70 CD Total 0 01 0 0, 0 0 0 0 1,365 5,278 0 0 2,566 0 0 0 0 0 70 Cl) White 0600-1800 9,188 0 0 0 0 0 0 0 1,050 14,527 0 16,103 8,376 2,876 0 0 0 0 4,018 sucker 1800-0600. 7,908 0 4,164 0 0 0 0 6,426 0 13,111 3,192 2,625 17,675 0 0 3,233 0 0 11,494 0 Total 17,095 0 4,164 0 0 0 0 6,426 1,050 27,638 3,192 18,728 26,051 2,876, 0 3,233 . 0 0 15,512 C, Yellow 0600-1800 361 2,360 14,373 59,375 9,564 289,450 44,170 98,894 258,226 108,895 41,509 30,591 20,831 9,609 22,077 2,991 189 84,470 322,907 perch 1800-0600 2,334 927 2,663 120,610 9,734 201,490 15,617 103,565 564,599 131,976 53,144 34,547 25,095 11,694 40,960 2,531 13 84,552 282,643 Total 2,695 3,287 17,037 179,985 19,298 490,940 59,787 202,459 822,825 240,871 94,653 65,138 45,926 21,303 63,037 5,522, 202 169,021 605,550 TOTAL 0600-1800 12,653 5,535 47,018 , 70,030 14,271 298,690 111,545 140,093 370,285 376,151 111,342 148,697 261,713 90,302 85,469 4,863 980 124,856 778,712 1800-0600 42,626 34,135 9,102 404,629 12,584 290,592 108,192 163,570 709,843 366,644 153,419 11.5,395 86,060 29,284 51,854 .11,623 4,656 108,817 616,618 Total 55,278 39,670 56,120 474,659 26,855 589,282 219,737 303,663 1,080,128 742,795 264,761 264,092 347,773 119,586 137,324 16,486 5,635 233,673 1,395,330 (continued)

Appendix Table I. (Continued) Ca.

C).

0 2007 0

Feb.06-Species Diel Jan Jan.07 X

DC Alewife 0600-1800 172 197,137 CD 1800-0600 550 44,983 (D

Total 722 242,120 Bloater 0600-1800 53 .1,964 1800-0600 0 544 Co 0.

0 Total 53 2,508 Q

0) Bluegill 0600-1800 0 401 1800-0600 226 937 Total 226 1,338 Blunt-nose minnow 0600-1800 0 14 m I-1800-0600 0 0 Total 0 14 Brook silver-side 0600-1800 0 0 1800-0600 0 14 Total 0 14 Brown bullhead 0600-1800 0 0 1800-0600 0 112 Total 0 112 Brown trout 0600-1800 0 86,936 1800-0600 0 20,810 Total 0 107,747 Burbot 0600-1800 21 4,673 1800-0600 146 1,243 Total 167 5,916 Z Central mudminnow 0600-1800 0 0 137 1800-0600 0 239 Total 0 376 Channel catfish 0600-1800 583 58,260 1800-0600 152 12,639 Total 735 70,899 Chestnut lamprey 0600-1800 0 0 1800-0600 0 0 0D Total 0 0 w

Chinook 0600-1800 0 25,522 i800-0600 0 49,858 Total 0 75,380 Coho 0600-1800 0 33,810 1800-0600 0 0 Total 0 33,810 (continued)

Appendix Table I. (Continued)

Mz 0- 2007 0

0 Feb.06-Species Diel 'Jan Jan.07 CO, CD, Common carp 0600-1800 0 10,507

-n 1800-0600 0 5,280 Total 0 15,787 Deepwater sculpin 0600-1800 0 383 0

1800-0600 0 446 rrn

-Ti Total 0 829 Eastern banded 0600-1800 0 82 killifish 1800-0600 0 0 Total 0 82 I-Flathead catfish 0600-1800 0 0 1800-0600 0 0 CO Total 0 0 Freshwater drum 0600-1800 0 5,236 1800-0600 0 10,516 Total 0 15,752 Gizzard shad 0600-1800 34,540 76,812 1800-0600 81,642 158,373 Total 1,16,182 235,185 Golden redhorse 0600-1800 2,528 12,142 1800-0600 0 2,670 Total 2,528 14,812 Golden shiner 0600-1800 0 0 1800-0600 0 0 Total 0 0 0 Greater redhorse 0600-1800 1,212 1,212 1800-0600 0 0 a) Total 1,212 1,212 Lake chub 0600-1800 0 0 1800-0600 0 0 a)

Total 0 0 Lake sturgeon 0600-1800 0 9,212 1800-0600 0 0 0 Total 0 9,212 C,

a) Lake trout 0600-1800 0 26,389 1800-0600 0 65,802 Total 0 92,191 Lake whitefish 0600-1800 38,026 325,899 1800-0600 143,969 349,456 Total 181,994 675,355 (continued)

N)

Appendix Table I. (Continued)

M

-P, :2~

CD 2007 0

0 Feb.06- 01 Species Diel Jan Jan.07 0CD Largemouth bass 0600-1800 0 0 CA 1800-0600 0 0 8

CD Total 0 0 5,

fD W Longnose dace 0600-1800 0 0 h-1 1800-0600 0 0 0

Total 0 0 flb Longnose sucker 0600-1800 43,545 260,536 co

-C 1800-0600 32,585 208,383 Total 76,130 468,918 Mi Mottled sculpin 0600-1800 0 69 1800-0600 0 40 -n Total 0 109 Ninespine stickleback 0600-1800 0 728 1800-0600 0 127 Total 0 855 Northern pike 0600-1800 0 363 1800-0600 0 0 Total 0 363 Pumpkinseed 0600-1800 0 0 1800-0600 0 782 Total 0 782 Rainbow smelt 0600-1800 910 8,283 1800-0600 1,085 15,754 Total 1,995 24,037 0 Rock bass 0600-1800 0 159 1800-0600 7 7 Total 7 166 Round goby 0600-1800 7,874 132,010 1800-0600 4,444 102,887 Total 12,317 234,897 Sea lamprey 0600-1800 16,059 78,061 1800-0600 7,547 29,508 0 Total 23,606 107,568 Q. Shorthead redhorse - 0600-1800 0 0 1800-0600 0 777 Total 0 777 Silver redhorse 0600-1800 0 0 1800-0600 0 0 1 Total 0

• 0 (continued)

0 Appendix Table 1. (Continued) 41 0 2007 0

0 Feb.06-Species Diel Jan Jan.07 ED, M, Slimy sculpin 0600-1800 0 3,199

-n 1800-0600 70 3,084 Total 70 6,283 PCA Smallwouth bass 0600-1800 0 25 1800-0600 0 0 Total 0 25 Spottail shiner 0600-1800 7,703 174,044 Clo 1800-0600 10,879 163,333 Total 18,581 337,377 Steelhead 0600-1800 0 12,338 1800-0600 0 4,650 Total 0 16,988 Threespine 0600-1800 55 700 stickleback 1800-0600 29 480 Total 84 1,181 Trout-perch 0600-1800 0 956 1800-0600 202 856 Total 202 1,812 Unidentified 0600-1800 0 0 1800-0600 0 0 Total 0 0 Walleye 0600-1800 0 0 1800-0600. 0 0 Total 0 0 0 White perch 0600-1800 747 9,956 5,' 1800-0600 2,079 2,149 w Total 2,827 12,105 White sucker 0600-1800 17,287 64,236 1800-0600 11,080 62,411 Total 28,367 126,647 Yellow perch 0600-1800 154,855 1,057,148 1800-0600 136,464 1,368,217 Co 0 Total 291,318 2,425,366 CS)

-S TOTAL 0600-1800 326,169 2,679,538 1800-0600 433,155 2,687,369 CD

,5n Total 759,324 5,366,907 p

4 Appendix Table J. Estimated Number of Fish Impinged at Cook Nuclear Plant Unit 2, Assuming Design Cooling Water Flow, June CN 2005 through January 2007 (June 2005 represents the impingement estimate only for the last week in June).

0 0

2005 . 2006 a),

Species Diel Jun I Jul Aug Sep Oct Nov Dec Jan Feb Mar Apr May Jun I Jul Aug I Sep Oct I Nov Dec 0)

U)

Alewife 0600-1806 13 0 7 273 87 70 0 54 84 0 0 730 28,243 430 160 80 370 1,251 531 CD 03 5: 1800-0600 0 56 0 2,940 43 413 70 35 68 31 20 556 2,654 362 378 60 200 4,030 509 CD

-n Total 13 56 7 3,213 130 483 70 89 152 31 20 1,286 30,897 793 538 140 569 5,281 1,040 Bloater 0600-1800. 0 0 126 931 81 140 315 0 14 13 8 0 0 0 4 11 49 0 126 0 1800-0600 0 0 40 420 285 138 392 147 18 0 0 0 0 0 0 14 222 84 68 Total 0 0 166 1,351 365 278 707 147 32 13 8 0 0, 0 4 25 2701 84 194 0

Bluegill 0600-1800 0 0 0 0 0 0 0 28 .14 169 113 0 0 0 0 0 4 14 21 rn 1800-0600 0 0 0 0 8 0 14 74 18 105 70 18 0 0 0 0 0 46 46 Total 0 0 -0 0 81 0 14 102 32 274 183 18 0' 0- 0 0 4 60 67 .fl Blunt-nose 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Co, minnow 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 -- 0 0 0 0 0, 0 0 0, 0 0 Brook 06004800 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 0 silverside 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 Total 0 0 .0 0 0 0 0 0 4 0 0 0 3 0 0 0 0 0 0 Brown bull- 0600-1800 0 0 0 0 0 0 0 0 0 14 10 4 0 0 0 0 0 head 1800-0600 0 0 0 0 0 0 0 0 0 13 9 0 0 0 0 0 4 0 0 Total 0 0 0 0 0 0 0 . 0- 0 27 19 4 0 0 0 0 4 0 0 Brown 0600-1800 0 14 0 0 0 .0 0 0 0 13 8 7 7 14 0 .0 0 0 trout 1800-0600 0 28 0 0 0 0 0 0 0 0 0 11 0 28 0 0 0 0 0 Total 0 42 0 0 0 0 0 0 0, 13 8 18 7 42 0 0 0 0 0 Burbot 0600-1800 0 0 0 0 0 0 0- 0 0 13 0 14 3 0 0 0 4 38 3 1800-0600 0 0 0 0 0 0 7 0 7 27 27 21 3 7 0 0 14 14 0 0 Total 0 0 0 0 0 0, 7 0 7 40 27 35 7, 7 0 0 18 52 3 Central 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 00 0 0 0 0 mud- 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 minnow Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Channel 0600-1800 0 0 0 0 0 0 0 56 147 131 109 18 0 0 13 0 0 7 48 catfish 1800-0600 0 0 0 0 8 0 56 70 161 149 64 21 0 0 14 0 0 19 1.19 (A Total 0 0 0 . 0 8 0 56 126 308 280 174 39 0 0 27 0 0 25 167 Chestnut 0600-1800 0 0 0. 0 0 0 0 0 0 0 0 0 0 0 '0 0 0 0 0 0 lamprey 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 01 0 0 0 0 0 0 0 0 0 .0 0 0 Chinook 0600-1800 0 0 03 01 0 0 0 0 18 26 8 0 0 0 0 0 0 0 0 CD 1800-0600 0 14 0 0 0 0 0 0 0 13 9 4 0 13 .0 0 4 0 0 I Total 0 14 0 0 0 0 0 0 18 39 17 4 0 13 00 4 0 0 (continued)

Appendix Table J. (Continued)

C) 0 0

Cr Species Diel Jun Jul J Aug f 2005 Sep I Oct Nov I Dec Jan Feb Mar Apr [May 2006 Jun I Jul I Aug Sep Oct I Nov Dec to Coho 0600-1800 7 0 0 0 0 0 0 0 0 22 12 0 0 0 0 0 0 0 0 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 Total 7 0 0 0 0 0 0 0 0 22 12 0 0 7 0 0 0 0 0 1h C.

0)

0Cr, Common 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13 0 0 0 0 carp 1800-0600 0 0 0 0 .0 0 0 0 0 0 0 0 0 C0 0 0 0 0 0 Cr, a Total 0 0 0 0 0 0 0 01 0 0 0 01 0 0 13, 0 01 0 0 Deepwater 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 rn sculpin 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 10 0 0 0 0 0 0 0 0 0 Eastern 0600-1800 0 0 0 0 0 0 35 0 0 0 0 .0 0 0 0 0 0 0 0 banded 1800-0600 0 0 0 0 0 0 0 0 0C 0 0 0 0 0 0 0 0 0 0 :l:

killifish Total 0 0 0 0 0 0 35, 0 0 0 0 0 0 0 0, 0 0 0 0 Flathead 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 catfish 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 M<

Total 0 0 0 0 0 0 0 0 0 0 *0 0 0 0 0 0 0 3 0 Freshwater 0600-1800 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 35 drum 1800-0600 0 0 14 0 0 0 56 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 21 01 0 0 56 0 0 0 0 01 0 0, 0 0 0 0 35 Gizzard 0600-1800 0 7 28 0 259 700 595 173 424 686 456 0 0 0 0 7 5,140 1,497 1,557 shad 1800-0600 .0 0 20 2,891 170 2,238 371 378 137 566 324 0 0 0 0 40 10,542 2,487 1,716 Total 0 7 48 2,891 430 2,938 966 551 560 1,252 780 0 0 0 0 47. 15,682 3,984 3,273 Golden 0600-1800 0 0 7 0 0 0 0 0 0 1 10 4 0 0 4 0 0 0 9 redhorse 1800-0600 0 0 0 0 0 0 0 0 0 1 10 0 0 0 0 0 0 0 0 Total 0* 01 7 0 01 0 0 .0 0 1 20 .4 0 0 4, 0 0, 0 9 Golden 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 shiner 1800-0600 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 -0 0 4 0 0 0 0 0 0 0 01 0 Greater 0600-1800 0 0 0 0 0 0 .0 0 0 0 0 0 0 0 0 0 0 0 0 redhorse 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 01 0 *1 0 0 01 0 0 0 0 0 0, 0 0 0 0 0 .0 01 Lakechub 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 .0 0 0 0 CD 1800-0600 0 0 0 0 0 0 0 0 0 0 0. 0 0 0 0 0. 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 01 0 0, 0 Lake 0600-1800 0 0 0 0 0 0 0, 0 0 0 0 0 0 0 .0 0 0 0 0 01 sturgeon 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 01 0 0 0 " 01 0 0, 0 0 0 0 0, 0 0 0 0 0 0 0 Lake trout 06 00-1800 0 0 7 0 0 0 0 0 0 0 0 0 7 0 0 0 4 7 9 1800-0600 0 0 0 0 8 0 .0 0 0 0 0 0 7 0 0 0 0 4 0 Total 0 01 7 0 8 0 0 0 0 -0 0 0 14 0 0 0 4 10 9 (continued)

Appendix Table J. (Continued) (A) 01 0 S u J2005 2006 N 0

0 Species DieOct Nov Dee Jan Feb MarIAprIMay Jun JulAug Sep Oct Dec C)

M W Lake 0600-1800 0 0 0 0 7 0 140 120 105 13 0 21 0 0 11 0 0 14 1,309 whitefish 1800-0600 0 0 0 0 0 140 161 88 384 50 9 4 0 0 0 0 0 64 2,247 CO)

CD 03 Total 0 0 0 0 7 140 301 207 489 63 9 25 0 0 11 0 0 78 3,556 rn CD

_n Largemout 0600-1800 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 7 0 0 h bass 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 01 0 0 Total 0 0 0 0 0, 0 0 0 7 0 0 0 0 0 07 0 CO) 0)

0. I-P

-I, Longnose 0600-1800 0 0 0 0 0 0 0 32 0 0 0 0 0 0 0 0 0 0 6 dace 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 mn Total 0 0 0 0 0 0 0 32 0 0 0 0 0 0 0 0 0 0 13 Longnose 0600-1800 20 0 0 0 7 0 0 - 0 32 75 39 34 43 13 0 0 7 0 19 sucker 1800-0600 0 0 0 0 8 0 7 77 39 32 0 47 53 0 0 0 0 0 32 Total 20 0 0 0 14 0 7 ,77 70 106 39 81 95 13 0 0 7 01 52 Mottled 0600-1800 0 0 0 0 0 0 0 0 0 0 0 4 4 0 0 0 0 0 0 sculpin 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0. 4 11 0. 0 0 0 0 0 Ninespine 0600-1800 0 0 0 0 0 0 0 0 14 19 22 35 7 0 0 0 .0 0 0 stickleback 1800-0600 0 .0 0 0 0 0 0 0 18 0 0 36 14 0 0 0 0 0 0 Total 0 0 0 0 01 0 0 0 32 19 22 70 21 0 0 0 0 0 0 Northern 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0. 0 0 0 0 pike 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 0, 0 0 0 0] 0 0 Pumpkinse 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ed 1800-0600 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 Total 0 0 0 0 01 0 0 0 0 0 0 4 0 0 0 0 0 0 0 Rainbow 0600-1800 0 0 392 42 14 0 105 119 438 466 441 283 25 0 13 0 4 67 210 0 smelt 1800-0600 0 14 81 0 8 280 84 305 343 362 280 72 10 0 11 0 32 14 259 Total 0 14 473 42 21 280, 189 424 780 828 721 355 35 0 24 0 35 81 469 Rock bass 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 Z

Total 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 Round 0600-1800 256 218 568 63 172 420 140 270 203 276 497 3,960 1,210 1,187 591 136 366 533 1,209

0. goby 1800-0600 478 261 193 574 142 2,518 392 340 229 367 419 3,289 970 752 236 129 590 837 736 Total 734 480 761 637 314 2,938 .532 610 432 643 916 7,249 2,180 1,939 827 265 956 1,370 1,945 0.

M Sea 0600-1800 0 0 0 0 0 0 0 0 49 11 0 11 0 0 0 0 0 0 0 lamprey 1800-0600 0 0 0 0 0 0 28 7 25 4 .0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 28 7 74 14 0 11. 0 0 0 0 0 0 0 Shorthead 0600-1800 0 0 0 0 7 0 0 0 7 0 0 0 0 0 0 0 0 0 0 redhorse 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

__1 Total 0 0 0 0 7 0 0 0 7 0 0 0 0 0 0 0 0 0 0 (continued)

Ia Appendix Table J. (Continued)

M 0

0 2005 2006 0

Species Diel Jun Jul Aug Sep Oct ov Dec Jan Feb Mar Apr May Jun [Jul Aug Sep Oct Nov Dec

,o C,, Silver 0600-1800 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0, X redhorse 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 Total 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 Slimy Co 0~ 0600-1800 0 0 0 0 0 0 0 4 70 19 22 42 35 0 0 0 0 0 0 0

sculpin 1800-0600 0 0 0 0 0 0 0 0 0 18 29 42 7 20 0 0 0 4 0 0,

Total 0 0 0 0 0 0 0 4 70 37 51 84 42 20, 0 0 0, 4 0 rnl C)

Small- 0600-1800 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0 0 m

).

Do

0. mouth bass 1800-0600 0 0 .0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 Total 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 0 4 0 Spottail 0600-1800 125 47 1,980 175 153 1,050 1,435 830 7,056 788 677 646 96 17 56 73 257 332 13,176 Co shiner 1800-0600 0 133 223 470 655 4,000 4,235 1,653 4,114 1,788 607 299 56 87 11 60 831 699 13,945 _n Total 125 180 2,203 645 808 5,050, 5,670 2,482 11,169 2,576 1,284 945 152 105 66 133 1,088 1,031 27,121 Steelhead 0600-1800 0 14 0 0 0 0 0 14 4 1 10 0 4 0 0 0 0 0 0 1800-0600 0 35 0 0 0 0 70 21 13 9 4 11 0 0 0 0 0 0 CO)

Total. 0 49 0 0 0.0 0 84 25 14 19 4 14 0 0 0 0 0 0 Threespine 0600-1800 0 0 0 0 0 0 0 35 200 73 29 24 4 0 0 0 0 14 17 stickleback 1800-0600 0 0 0 0 0 0 28 84 186 23 9 50 25 0 0 0 0 0 .7 Total 0 0 0 0 0 0 28 119 385 97 38 75 28' 0 0 0 0, 14 24 Trout- 0600-1800 0 0 0 0 0 0 0 0 7 4 0 7 0 0 0 0 7 0 85 perch 1800-0600 0 0 0 0 0 140 70 7 39 26 18 7 0 0 0 0 7 0 65 Total 0 0 0 0 0 140 70 7 46 30 18 14 0 0 0 0 14 0 150 Unidenti- 0600-1800 0 0 14 0 0 0 0 0 0 18 12 0 0 0 0 0 0 0 0 fied 1800-0600 0 7 0 0 0 .0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 7 14 0 0 0 0 0 0 18 12 0 .0 0 0 0 0 0 0 Walleye 0600-1800 0 0 7 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 -0 0 0 Total 0 0 7 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 White 0600-1800 0 0 0 0 0 0 0 0 0 31 20 0 0 0 0 0 0 0 3 perch 1800-0600 0 0 0 0 0 0 0 0 0 13 9 0 0 0 0 0 0 0 0 Total 0 01 0 0 0 0 0 0 0 44 29 0 0 0 01 0 0 0 3 White 0600-1800 7 7 0 7 0 0 0 63 11 11 0 42 39 0 0 4 4 0 0 sucker 1800-0600 0 0 0 0 7 0 7 0 0 .33 18 36 10 7 0 0 11 0 43 (0 Total 7 7 0 7 7 0 7 63 11 44 18 77 49 7 0 4 14 0 43 CO 0 Yellow 0600-1800 53 40 12,408 5,201 1,798 83,510 6,615 34,612 218,077 21,934 12,947 6,327 1,040 167 2,149 420 1,182 14,816 122,230 perch 1800-0600 49 154 440 7,337 2,428 70,294 13,286 29,416 134,757 31,240 8,806 6,552 1,001 414 1,701 318 1,092 11,558 41,265 U)

Q Total 102 194 12,848 12,538 4,226 153,804 19,901 64,029 352,834 53,174 21,753 12,878 2,041 581 3,850 .739 2,274 26,375 163,495

,50 TOTAL 0600-1800 479 353 15,551 6,692 2,590 85,890 9,380 36,410 226,987 24,825 15,451 12,210 30,766 1,828 3,021 731 7,403 18,593 140,605 1800-0600 527 704 1,010 14,633 3,768 80,160 19,264 32,749 140,560 34,876 10,743 11,071 4,839 1,698 2,350( 621 13,553 19,862 61,065

_ Total 1,006 1,056 16,561 21,325 6,357 166,050 28,644 69,159 367,548 59,701 26,194 23,281 35,604 3,526 5,371 1,352 20,955 38,454 201,669 (continued)

4N Appendix Table J. (Continued)

Ln 2

2007 0,

C) Feb.06-Species Diel Jan Jan.07 Alewife 0600-1800 40 31,921 1800-0600 105 8,972 0)

C, Total 146 40,893 Bloater 0600-1800 11 *235 1800-0600 0 406 Co M

Total 11 640 Bluegill 0600-1800 4 338 1800-0600 20 323 Total 24 661 Bluntnose minnow 0600-1800 0 0 1800-0600 0 0 Total 0 0 Brook silverside 0600-1800 0 4 CA 1800-0600 0 3 Total 0 7 Brown bullhead 0600-1800 0 27 1800-0600 0 25 Total 0 53 Brown trout 0600-1800 19 67 1800-0600 0 39 Total 19 106 Burbot 0600-1800 35 111 1800-0600 21 142 Total 56 253 Z Central mudminnow 0600-1800 0 0 1800-0600 0 0 Total 0 0 a Channel catfish 0600-1800 27 500 1800-0600 7 554 Total 34 1,054 (I)

Chestnut lamprey 0600-1800 21 21 1800-0600 0 0 0 Total 21 21 0

Chinook 0600-1800 0 52 C) 1800-0600 0 42 Total 0 94 Coho 0600-1800 0 34 1800-0600 0 7 Total 0 41 (continued)

N) Appendix Table J. (Continued) CA C) 0 2007 0

Feb.06-

-01 Species Diel Jan Jan.07 Common carp 0600-1800 0 13 CD 1800-0600 0 0 Total 0 13 0*

a Deepwater sculpin 0600-1800 0 0 hi rn 1800-0600 7 7 Total 7 7 l.

Eastern banded 0600-1800 0 0 CO, to 4* killifish 1800-0600 0 0 Total 0 0 Flathead catfish 0600-1800 14 17 1800-0600 0 0 M Total 14 17 Freshwater drum 0600-1800 0 35 0

1800-0600 0 0 Total 0 35 Gizzard shad 0600-1800 341 10,107 0, 1800-0600 229 16,040 Total 570 26,147 0 Golden redhorse 0600-1800 0 27 1800-0600 .0 11 Total 0 38 Golden shiner 0600-1800 0 0 1800-0600 0 4 Total 0 4 Greater redhorse 0600-1800 0 0 1800-0600 0 0 Total 0 0 Lake chub 0600-1800 0 7 1800-0600 0 0 Total 0 7 Lake sturgeon 0600-1800 0 0 1800-0600 0 0 Total 0 0 Lake trout 0600-1800 4 30 1800-0600 0 11 Total 4 41 Lake whitefish 0600-1800 277 1,750 1800-06.00 473 3,230 Total '750 4,980 (continued) .

Appendix Table J. (Continued) W N) 0 2007 Feb.06-CO Species Diel Jan Jan.07 CD

3 Largemouth bass 0600- 0 14 1800 0

0v 1800- 0 0 0600 0.

Total 0 14 rto Longnose dace 0600- 0 6 1800 0 1800- 0 7

)b, 0600 Total 0 13 Longnose sucker 0600- 4 264 1800 -CO 1800- 0 202 0600 Total 4 466

'Mottled sculpin 0600- 0 7 1800 1800- 0 7 0600 Total 0 14 Ninespine 0600- 7 104 stickleback 1800 1800- 0 67 0600 Total 7 171 Northern pike 0600- 0 0 1800 1800- 0 0 0 0600 Total 0 0 Pumpkinseed 0600- 0 0 1800 1800- 0 4 0600 Total 0 4 Rainbow smelt 0600- 354 2,299 1800 0 1800- 1,312 2,694 a, 0600 Total 1,666 4,993 Rock bass 0600- 7 7

'5, 1800 1800- 0 7 0600 Total 7 14 (continued)

N) Appendix Table J. (Continued)

CD N,

Cn 03 0 2007 0,

0)

Feb.06-Species Diel Jan Jan.07 w

Round goby 0600- 342 10,510 )h 1800 CD 1800- 188 8,741 C/)

21 0600 Total 530 19,251 20.

0 Sea lamprey 0600- 39 109 0 1800 CD 1800- 11 39 0600 Total 49 147 rn Shorthead 0600- 0 7 redhorse 1800 1800- 0 0 0600 Total 0 7 Silver redhorse 0600- 0 0 1800 1800- 0 7 0600 Total 0 7 Slimy sculpin 0600- 0 188 1800 1800- 0 120 0600 Total 0 308 Smallmouth bass 0600- 0 7 1800 1800- 0 4 z 0600 Total 0 11 Spottall shiner 0600- 1,139 24,313 1800 1800- 2,161 24,657 0600 Total 3,300 48,970 CD Steelhead 0600- 14 31 1800 Co 0 1800- 11 68 0600 Total 25 99 Threespine 0600- 35 396 stickleback 1800 1800- 59 359 0600 Total 94 755 (continued)

N)

C) Appendix Table J. (Continued)

.91 0

0 2007 Feb.06-Species Diel Jan Jan.07 CD)

Trout-perch 0600- 0 110 1800 1800- 0 161 0*

0600 C', Total 0 271 Unidentified 0600- 0 30 1800 1800- 0 0 C,,

0600 I'l Total 0 30 Walleye 0600- 0 0 1800 1800- 0 0 0600 Total 0 0 White perch 0600- 0 55 1800 1800- 0 22 0600 Total 0 77 White sucker 0600- 81 189 1800 1800- 13 171 0600 Total 94 360 Yellow perch 0600- 20,258 421,548 1800 1800- 13,469 252,172 0600 Total 33,727 673,720 TOTAL 0600- 23,069 505,489 1800 1800- 18,086 319,323 (D

0600 Total 41,155 824,812 0

SAppendix Table K. Estimated Biomass (g) impinged at Cook Nuclear Plant Unit 2, Assuming Design Cooling Water Flow, June 2005 0) through January 2007 (June 2005 represents the biomass impingement estimate only for the last week in June).

0

-O S12005 2006 0)

CY Species Diel J Feb Mar[Apr May Jun[Jul Aug SepIOctJNov Dec CO CO Alewife 0600-1800 289 0 7 532 293 140 0 171 270 0 0 8,469 189,752 3,171 1,548 381 2,040 3,010 1,342 CD 1800-0600 0 1,589 0 5,740 541 689 350 81 1,106 132 186 5,466 73,984 2,674 4,749 286 833 9,674 1,254 Total 289 1,589 7 6,272 834 829 350 252 1,376 132 186 13,936 263,736 5,845 6,297 667 2,874 12,684 2,596 a)

Bloater 0600-1800 0 0 168 1,995 241 560 945 0 70 25 17 0 0 0 11 21 147 .0 569 a.

0 1800-0600 0 0 67 910 550 964 1,771 4,417 70 0 0 0 0 0 0 43 686 154 244 Total 0 0 235 2,905 792 1,524 2,716 4,417 140 25 17 0 0 0 11 64 833 154 813 rrn Bluegill 0600-1800 0 0 0 0 0 0 0 56 42 721 472 0 0 0 0 0 4 28 93 1800-0600 0 0 0 0 23 0 56 217 18 1,157 771 402 0 0 0 0 0 277 134 Total 0 0 0 .0 23 0 56 273 60 1,878 1,244 402 0 0 0 0 4 305 227 Bluntnose 0600-1800 0 0 0 0 0 0 0 0 0, 0 0 0 0 0 0 0 0 0 0 minnow 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 go Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 01 0 0 0 Brook 0600-1800 01 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 1 0 0 0 silverside 1800-0600 01 0 0 0 0 0 0. 0 0 0 0 0 17 0 0 0 0 0 0 -l<

Total 0 0 0 0 0 0 0 0 7 0 0 0 17 0 0 0 0 0 0 Brown 0600-1800. 0 0 0 0 0 0 0 0 01 162 39 270 0 0 0 0 0 0 0 bullhead 1800-0600 0 0 0 01 0 0 0 0 0 39 26 0 0 0 0 0 11 0 0 Total 0 0 0 0 01 0 0 0 0 201 65 270 0 0 0 0 11 0 0 Brown 0600-1800 0 21,943 0 0 01 0 0 0 0 1,751 1,168 10,971 3,637 9,899 01 0 0 0 0 trout 1800-0600 0 48,625 .0 0 0 0 0 0 0 0 0 64 0 15,014 0 0 0 0 0

0. Total 0 70,568. 0 0 0 0 0 0 0 1,751 1,168 11,035 3,637 24,913 0 0 0 0 0 Burbot 0600-1800 0 0 01 0 0 0 0 0 01 40 0 90 831 0 0 0 18 601 31 1800-0600 0 0 0 0 0 0 42 0 37 134 117 128 41 416 0 0 224 210 0 Total 0 0 0 0 0 0 42 0 37 173 117 218 124 416 0 0 242 811 31 Central 0600-1800 0 0 0 .0 0 0 0 0 0 0 0 0 0 0 0, 0 0 0 0 mud- 1800-0600 0 0 0 01 0 {

0, 0O. 0 0 0, 0 0 o 0 0 o01 minnow Total 0 0 0 0 0 0 01 0 0 0 0 0 0 0 0 0 0 0 0 0 Channel 0600-1800 0 0 01 0 0 0 0 112 336 14,447 8,602 46 0, 0 4,269 0 0 68 10,529 catfish 1800-0600 0 0 0 0 23 0 19,880 4,690 9,251 626 149 46 0 0 400 0 0 815 24,070 Total 0 0 0 0 23 0 19,880 4,802 9,587 15,073 8,750 92 0 0 4,670 0 0 883 34,599 en Chestnut 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 lamprey 1800-0600 0 0 0 0 0 0 0 0 0 01 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Q9 Chinook 0600-1800 0 0 01 0 0 0 0 0 3,185 25,628 9,358 0 0 0 0 0 0 0 0 PD 1800-0600 0 2331 01 0 0 0 0 0 0 13,832 9,221 15 0 413 0 0 202 00 Total 0 233 0 0 0 0 0 0 3,185 39,460 18,579 15 0 413 0 0 202 01 0 (continued)

C.o Appendix Table K. (Continued)

I'3 0

0 2005 1*2006 0

CD Species Diel JunI Jul jAug Sep I Oct Nov Dec Jan [Feb Mar Apr May [Jun, IJul I Aug Sep Oct Nov[ Dec Coho 0600-1800 3,747 0 0 0 0 0 0 0. 0 19,998 11,685 0 0 0 0 0 0 0 0 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 7,147 0 0 0 0 0 0

-n Total 3,747 0 0 0 0 0 0 0 0 19,998 11,685 0 0 7,147 0 -0 0 0

0 0, "I,

Common 0600-1800 0- 0 0 0 0 "0 0 0 0 0 0 0 0 0 32,032 0 0 0 0 carp 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 a.

0 Total 0 00 0 0 0 0 00 0 0 0 .0 0 0 032,032 0 0 0 0 rrl Deepwater 0600-1800 0 0 0 0 0 0 0 0 '0 0 0 0 0 0 0 0 0 0 0 sculpin" 1800-0600 0 0" 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Eastern 0600-1800 0 0 0 0 0 0 315 0 0 0 0 0 0 0 0 0 0 0 0 banded 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0' 0 0 0 0 0 0 '0 killifish Total 0 0 0 0 0 .0 315 0 0 0 0. 0 .0 0 0 0 0 0' 0 Flathead 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2,434.. 0 catfish 1800-0600 0 0 0 -0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 CO)

Total 0 0 0 0 0 0 0 0 q*

0 0 0 0 0 0 0 0 0 2,434 0 Freshwater 0600-1800 0 0.- 4,599 0 0 0 0 01 0 0 0 0 0 0 0 0 0 0 63,570 drum 1800-0600 0 . 27,852 0 0 0 75,404 0 0 01 0 01 0 0 0 .0 0 0 .0 Total 0 0 32,451 0 .0 0 75,404 0 0 0 0 0 0 0 0 0 0 0 63,570 Gizzard 0600-1800 0 5,271 . 105 0 1,566 . 3,570 3,010 7,742 ý6,510 18,851 3,849 0 0 0 0 466 96,615 10,59.8 8,846 shad 1800-0600 0 0 40 11,306 736 9,222 2,296 4,631 1,306 29,132 3,506 0. 0 0 0 240 74,793 27,896 .11,251 Total 0 5,271 145 11,306 2,302 12,792 5,306 12,373 7,816 .47,983 7,356 0 0 0 0 706 171,409 38,494 20,097 Golden 0600-1800 0 0 .14 - 0 0 0 01 0 0 1,064 14,896 5,163 01 0 8,221 .0 0 0 1,644 redhorse 1800-0600 0' 0 0 0 0 0 0 0 -0 2 29, 0 0 0 0 0 0 0 0 Total 0. 0 14 0 0 0 0 0 0 1,066 14,925 5,163 0 0 8,221 0 0 0 1,644 0 Golden 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

-'I shiner 1800-0600 0 0 0 0 0 0 0 0 0 123 0 0 -0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 123 0 0 0 0 0 0 0 0 0 w

Greater 0600-1800 0 0 0. 0 0 0 0 0 0 0 0 01 0 0 0 0 0 0 0 redhorse 1800-0600 0 0 0 0 0 0 0 0 0 *0 0 0 0 0 0 0 0 0, 0 Q

Total 0 0 0 0 '0 0 0 0 0 0 0 0 0 0 0 0 0 0 01 a) Lakechub 0600-1800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 196 0 0 0 0 C', 1800-0600 0 0 0 0 0 0 0 0 0 .0 0 0 0 0 0 0 0 0 0 C',

0 'Total 0 0 .0 0 0 0 0. 0 0' 0 0 0 01 0 196 0 0 0 v 0 C., Lake 0600-1800 0 0 0 0 0 0 0 0 0 0 0* 0 0 0 .0 0 0 0 0 a)

-I. sturgeon 1800-0600 0 0 0. 0. 0 0 0 0 0 0 0 0 0 0 0 0 0 01 0 CD Total 0 0 0 0 0

.5', 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Lake trout 0600-1800 0 0 147 0 0 0 0 0 0 0 0 0 161 0 0 0 12,285 30,307 29,768 p 1800-0600 0 0 0 0 144 0 0 0 0 0 0 0 112 0 0 0 0 8,291 0 Total 0 0 147 0 144 0 0 0 0 '0 0 0 273 0 0 0 12,285 38,598 29,768 (continued)

Appendix Table K. (Continued)

CO) 0* 2005 2006 0

0)

Species Diel Jun Jul Aug Sep j Oct Nov I Dec Jan Feb I Mar I Apr May Jun I Jul I Aug Sep Oct Nov Dec Lake- 0600-1800 0 0 0 0 3,252 0 176,540 30,841 59,465 15,929 0 - 235 0 0 2,342 0 0 6,782 210,722 CD, whitefish 1800-0600 0 0 0 0 0 66,360 94,500 16,104 126,178 15,206 1,078 5,478 0 0 0 0 0 6,258 339,964 r-01 Total 0 0 0 0 3,252 66,360 271,040 46,945 185,643 31,135 1,078 5,713 0 0 2,342 0 0 13,039 550,686 0~

Largemout 0600-1800 0 0 0 0 0 0 0 0 266 0 0 0 0 0 0 0 28 0 0 ZZ a, hbass 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Ca Total 0 0 0 0 0 0 0 0 266 0 0 0 0 0 0 0 28 0 0 4n Longnose 0600-1800 0 0 0 0 0 0 0 308 0 "0 0 0 0 0 0 0 0 0 24 dace 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14 Total 0 0 0 0 0 0 0 308 0 0 0 0 0 0 0 0 0 0 38 Longnose 0600-1800 14,792 0 0 0 6,828 0 0 0 34,626 96,820 42,565 27,582 38,655 8,941 0 0 6,393 0 24,136 sucker 1800-0600 0 0 0. 0 7,200 0 1,498 114,436 45,497 36,069 0 46,542 51,075 0 0 . 0 0 0 34,135 rr, Total 14,792 0 0 0 14,028 0 1,498 114,436 80,122 132,889 42,565 74,124 89,729 8,941 0 0 6,393 0 58,270 In 00 Mottled 0600-1800 0 0 0 0 0 0 0 0 0 0 0 42 35 0 0 0 0 0 0(

sculpin 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 77 0 0 0 0 0 0 CO)

Total 0 0 0 0 0 0 0 0 0 0 42 112 0 0 0. .0 0 0 Ninespine 0600-1800 .0 0 0 0 0 0 0 0 35 38 54 98 18 0 0 0 0 0 0 stickleback 1800-0600 0 0 0 0 0 0 0 0 35 0 0 103 35 0 0 0 0 0 0

,> Total 01 0 0 0 .0 0 0 .0 70 38 54 201 52 0 0 0 0 0 0 Northern 0600-1800 0( 0 0 0 0 0 0 0 (0 0 10 0 0 0 0 0 0 0 0 pike 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 "0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Pumpkinse 0600-1800 0 0 0 0 0 0 0 0 0 0 0. .0 0 0 . 0 0 0 0 ed 1800-0600 "0 0 0 0 0 .0 0 0 0 0 01 32 0 0 0 0 0 0 0 Total 0 .0 0 0 0 0 0 0 0 0 0 32 0 0 0 0 0 0 -0 Rainbow 0600-1800 01 0 1,771 637 82 0 . 420 347 753 1,183 1,143 353 25 0 132 0 165 396 1,357 smelt 1800-0600 0 35 430 -- 0 129 5,460 700 767 846 730 638 176 21 0 11 0 137 336 2,378 Total 0 35 2,201 637 211 5,460 1,120 1,114 1,598 1,913 1,781 528 46 0 142 0 301 732 3,735 Rockbhass 0s600-180 -0..

. . 0..... . o 3. - 0 -.C (. .0 0 0 0 - 0 - 0 1800-0600 0 01 0. 0 0 0 0 0 0 0 A0 0 1,904 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 0 0 0 1,904 0 0 0 0 0 0 Round 0600-1800 4,607 3,223 9,043 1,281 3,092 4,830. 875 3,049 2,422 2,135 6,966 56,833 15,927 14,060 7,198 1,728 9,141 10,277 20,808 goby 1800-0600 6,460 3,328 2,559 6,974 2,978 47,997 4,676 6,973 3,512 2,874 5,717 53,533 12,040 8,254 2,969 2,944 12,937 21,046 10,496 0

Total 11,067 6,551 11,602 8,255 6,070 52,827 5,551 10,023 - 5,934 5,009 12,683 110,365 27,967 22,314 10,167 4,672 22,078 31,323 31,304 0 Sea 0600-1800 0 0 0( 0 0. -0 0 0 18,981 3,700 0 210 0 0 0 0 0 0 0 lamprey 1800-0600 0 0 0 0 0 0 11,844 3,164 7,623 690 - .0 0 0 0 0 .0 0 0 0 Total 0 0 0 0 0 0 11,844 3,164 26,604 4,389 0 210 0 0 0 0 0 0 0 C;.) Shorthead 0600-1800 0 0 _0 01 0,7301 0 0 0 2,289 0 0 0 0 0 0 0 0 0 0 redhorse 1800-0600 0 0 0 0 01 0 0 0 0 0 0 . 0 0 0 0 0 0 0 0 Total 0 0 0 01 10,7301 0 0 0 2,289 0 0 0 0 0 0 0 0 0 0 (continued)

Appendix Table K. (Continued)

C,,

N)j C) 0

• 2005 - 2006 CO) 0 Species Diel [eJun Jul IAu Sep Oct Nov Dec Jan Feb Mar Apr May Jun Jul Aug ISep Octc

'C-Silver 0600-1800 0 16,471 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 redhorse 1800-0600 0 0 0 0 0. 0 0 0 0 0 0 0 0 0 0 0 28 0 0 CD

_n Total 0 16,471 0 0 0 0 0 0 0 0 0 0 0 0 0 0 28 0 0 CO) m

0) Slimy 0600-1800 0 -0 0 0 0 0 0 11 350 112 122 391 358 0 0 0 0 0 0 sculpin 1800-0600 0 0 0 0 0 0 0 0 0 101 158 335 70 140 0 0 0 14 0 CD Total 0 0 0 0 0 0 0 11 350 214 279 726 428 140 0 0 01 14 0 4D Smallmout 0600-1800 0 0 0 0 0 0 0 0 3,143 0 0 0 0 0 0 0 0 0 0 h bass 1800-0600 0 0 0 0 0 0 0 0 0 0 0 . 0.0 0 0 0 14 0 Total 0 0 0 0 0 0 0 0 3,143 0 0 0 0 0 0 0 0 14 0 Spottail 0600-1800 1,247 266 13,330 1,323 735 4,970 15,330 5,331 53,738 6,584 5,298 7,120 1,127 210 489 314 1,077 2,279 76,905 shiner 1800-0600 0 1,338 1,404 3,157 1,918 38,046 39,137 9,345 31,309 14,721 4,512 3,049 654 860 63 378 3,258 4,078 59,750 rn Total 1,247 1,604 14,734 4,480 2,654 43,016 54,467 14,676 85,047 21,305 9,810 10,169 1,781 1,070 552 692 4,336 6,356 136,655 Steelhead 0600-1800 0 14,421 0 0 0 0 0 3,430 5,607 694 9,722 0 46 0 0 0 0 0 0 Co 1800-0600 0 41,314 0 0 0 0 0 52,640 3,318 2,761 1,841 4,260 147 0 .0 0 0 0 0 Total 0 55,734 0 0 0 0 0 56,070 8,925 3,455 11,562 4,260 193 0 0 0 0 0 0 Threespine 0600-1800 0 0 0 0 0 0 0 67 270 99 37 38 4 0 0 0 0 14 31 stickleback 1800-0600 0 0 0 0 0 0 28 91 238 27 9 79 42 0 0 0 0 0 11 Total 0 0 0 0 0 0 28 158 508 126 46 117 46 0 0 0 0 14 42 Trout- 0600-1800 0 0 0 0 0 0 0 0 49 18 0 34 0 0 0 0 21 0 703 perch 1800-0600 0 0 0 0 0 840 532 49 214 131 88 29 0 0 0 0 39 0 424 Total 0 0 0 0 0 840 532 49 263 149 88 64 0 0 0 0 60 0 1,128 Unidenti- 0600-1800 0 0 14 0 0 0 0 0 0 272 181 0 0 0 0 01 0 0 0 fled 1800-0600 0 21 0 0 0 0 01 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 21 14 0 0 0 0 0 0 272 181 0 0 0 0 0 0 0 0 Walleye 0600-1800 0 0 1,694 0 5,203 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1800-0600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Total 0 0 1,694 0 5,203 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Z White 0600-1800 0 0 0 0 *0 0 0 0 0 3,327 2,218 0 0 0 0 0 0 0, 14 perch 1800-0600 0 0 0 0 0 0 0 0 0 1,288 859. 01 0 0 0 0 0 0 0 Total 0 0 0 0 0 0 0 0 0 4,615 3,077 0 0 0 .0 0 0 0 14 White 0600-1800 8,531 3,086 0 9,317 0 0 0 24,933 2,090 14,315 0 42,342 36,638 0 0 2,891 3,196 0 0 sucker 1800-0600 0 0 0 0 8,750 0 4,466 0 0 28,677 14,568 35,844 8,769 5,716 0 0 2,942 0 29,695 Total 8,531 3,086 0 9,317 8,750 0 4,466 24,933 2,090 42,992 14,568 78,187 45,407 5,716 0 2,891 6,137 0 29,695 0

Yellow 0600-1800 827 523 27,426 30,639 8,313 377,020 24,780 157,472 706,140 94,705 50,431 30,450 18,274 5,993 40,048 4,861 9,183 134,997 567,130 perch 1800-0600 2,256 9,696 2,869 30,222 7,296 343,512 57,106 137,566 525,951 125,882 32,977 52,970 35,808 12,419 18,127 1,819 15,457 113,493 608,752 Total 3,083 10,220 30,295 60,861 15,610 720,532 81,886 295,038 1,232,091 220,587 83,407 83,420 54,082 18,411 58,175 6,680 24,640 248,490 1,175,882 TOTAL 0600-1800 34,040 65,203 58,318 45,724 40,336 391,090 222,215 233,870 900,641 322,620 168,821 190,736 304,739 42,274 96,485 10,662 140,312 201,790 1,018,221 1800-0600 8,716 106,179 35,221 58,309 30,288 513,089 314,286 355,171 756,506 274,333 76,449 208,551 184,795 53,051 26,320 5,710 111,547 192,556 1,122,573 1 Total 42,756 171,382 93,539 104,033 70,624 904,179 536,501 589,042 1,657,147 596,952 245,270 399,287 489,534 95,325 122,804 16,372 251,859 394,347 2,140,794 (continued)

N)

C)

Appendix Table K. (Continued) 01 01 N) 2007 0

Feb.06-Species Diel Jan Jan.07 Alewife 0600-1800 362 210,346 05.,

1800-0600 301 100,646 CD 0~ Total 664 310,991 C) Bloater 0600-1800 64 923 W

0h.

1800-0600 0 1,198 rJn DC Total 64 2,121

0. Bluegill 0600-1800 4 1,364 Ci 1800-0600 20 2,778 Total 24 4,142 Bluntnose minnow 0600-1800 0 0 (I-1800-0600 0 0 Total 0 0 Brook silverside 0600-1800 0 7 1800-0600 0 17 Total 0 24 Brown bullhead 0600-1800 0 471 1800-0600 0 76 Total 0 547 Brown trout 0600-1800 12,378 39,804 1800-0600 0 15,078 Total 12,378 54,882 Burbot 0600-1800 10,983 11,845 1800-0600 244 1,549 Total 11,227 13,394 Central 0600-1800 0 0 mudminnow 1800-0600 0 0

-- Total _ 0 0 Channel catfish 0600-1800 952 39,249 0 1800-0600 27 35,384 Total 979 74,633 Chestnut lamprey 0600-1800 924 924

0) 1800-0600 0 0 0

C, Total 924 924 Chinook 0600-1800 0 38,171 1800-0600 0 23,682 Cb Total 0 61,853 Coho 0600-1800 0 31,683 1800-0600 0 7,147 Total 0, 38,830 (continued)

Appendix Table K. (Continued) 0, 0

0 2007 rtO Feb.06- CY)

Species Diel Jan Jan.07 Common carp 0600-1800 0 32,032 C/

rn C-n 1800-0600 0 0 0* Total 0 32,032 Deepwater sculpin 0600-1800 0 0 Z-.

1800-0600 112 112 Total 112 112 Eastern banded 0600-1800 0 0 rn killifish 1800-0600 0 0 Total 0 0 Flathead catfish 0600-1800 17,278 19,713 Co 1800-0600 0 0 Total 17,278 19,713 Freshwater drum 0600-1800 0 63,570 PC Wl 1800-0600 0 0 Total 0 63,570 Gizzard shad 0600-1800 30,958 176,693 1800-0600 25,049 173,174 Total 56,006 349,866 Golden redhorse 0600-1800 0 30,988 1800-0600 0 32 Total 0 31,020 Golden shiner 0600-1800 0 0 1800-0600 0 123 Total 0 123 Z Greater redhorse 0600-1800 0 0 1800-0600 0 0

0. Total 01- 0 Lake chub 0600-1800 0 196 1800-0600 0 0 Total 0 196 Q'

0) Lake sturgeon 0600-1800 0 0 1800-0600 0 0 Total 0 0 0 Lake trout 0600-1800 7,614 80,135 1800-0600 0 8,403 Total 7,614 88,537 Lake whitefish 0600-1800 58,543 354,017 1800-0600 117,880 612,042 Total 176,423 966,0591 (continued)

M Appendix Table K. (Continued)

M co 0

2007 0., _I Feb.06-CD Species Diel Jan Jan.07 0" Largemouth bass 0600-1800 0 294 Cn 0 1800-0600 0 0 rn Total 0 294 Longnose dace 0600-1800 0 24 1800-0600 0 14 Cn Total 0 38 rn Longnose sucker 0600-1800 5,962 285,679 1800-0600 0 213,317 Total 5,962 498,996 Mottled sculpin 0600-1800 0 77 1800-0600 0 77 CO)

Total 0 154 Ninespine 0600-1800 14 256 stickleback 1800-0600 0 173 Total 14 .429 Northern pike 0600-1800 0 0 0,

1800-0600 0 0 Total 0 0 Pumpkinseed 0600-1800 0 0 1800-0600 0 32 Total 0 32 Rainbow smelt 0600-1800 451 5,954 1800-0600 1,841 7,114 Total 2,292 13,068 Rock bass 0600-1800 529 529 1800-0600 0 . 1,904

- . ..- Total - 529 2,433 Round goby 0600-1800 4,810 152,305 1800-0600 2,183 138,504 Total 6,993 290,809 Sea lamprey 0600-1800 14,006 36,896 1800-0600 3,744 12,057 Total 17,750 48,952 Shorthead 0600-1800 0 2,289 redhorse 1800-0600. 0 0 Total 0 2,289 Silver redhorse 0600-1800 0 0 1800-0600 0 28 Total 0 28 (continued)

Appendix Table K. (Continued)

M al 0

01 2007 0.

Jan Feb.06-Species Diel Jan.07 Slimy sculpin 0600-1800 0 1,333 1800-0600 0 819 Total 0 2,152 r-C,,

Smallmouth bass 0600-1800 0 3,143 0 1800-0600 0 14 (n

0 Total 0 3,157 Spottail shiner 0600-1800 7,834. 162,974 1800-0600 11,958 134,589 Total 19,792 297,563 Tn Steelhead 0600-1800 2,416 18,485 1800-0600 149 12,477 Total 2,566 30,961 rhreespine 0600-1800 35 528 stickleback 1800-0600 69 475 Total 104 1,003 Trout-perch 0600-1800 0 825 1800-0600 0 925 Total 0 1,750 Unidentified 0600-1800 0 454 1800-0600 0 0 Total 0 454 Walleye 0600-1800 0 0 1800-0600 0 0 Total 0. 0 White perch 0600-1800 0 5,559 1800-0600 0 2,147 Total 0 7,706 White sucker 0600-1800 13,675 115,146 0 1800-0600 2,096 128,305 Total 15,770 243,451 Q. Yellow perch 0600-1800 177,166 1,839,378 0 1800-0600 148,865 1,692,519 C, Total 326,031 3,531,897 P

TOTAL 0600-1800 366,956 3,764,256 1800-0600 314,538 3,326,929 E9g Total 681,494 7,091,185

4 r-n Appendix Table L. Mean Density (No./100 i 3) of Ichthyoplankton collected at the Shoreline, Intake, and Experimental Stations in the 0 vicinity of Cook Nuclear Plant, June through November 2005 and April through November 2006.

00 1-N Station Experimental, 30-40 Experimental, surface -

(.3 0D feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline rrn Diel Diel Diel Diel Diel Diel 0~

Day Night Day Night Day Night Day Night Day Night Day (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night CO) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) (1800-0600) 25 4I Density Density Density Density Density Density - Density3 Density3 Density3 Density3 Density3 Density3 3 3 3 /100 m

/100 m /100mW 1100M /100mW /100m /10m /100m /100 m /100 m /100 m /100 m Jun- Alewife Egg 0 0 0 0 0 05 Yolk-sac 0 0.6 0 0 0 rn.

larvae Post-yolk-sac 0 1.9 0 0 9.2 larvae Common Post-yolk-sac 0 0 0 0 0 PC, carp larvae Cyprinidae Unidentified 1.6 0 0 0 0 Yolk-sac 0 0 0 0 0 larvae Post-yolk-sac 0 0 0 0 0 larvae Round Post-yolk-sac 0 0 0 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 1.6 0.6 0 0 0 perch larvae Post-yolk-sac 4.1 2.5 0 0 0 larvae Total 7.3 5.7 0 0 9.2 (continued) 0 0

Appendix Table L. (Continued)

M3 0

0 Station Experimental, 30-40 Experimental, surface -

feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline co Diel Diel Diel Diel Diel Diel Mo Day Night Day Night Day Night Day Night Day Night Day Z_.

0, (0600- (1800- (0600W (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night CD 1800)

Density 0600)

Density 1800)

Density 0600)

Density 1 1800)

Density 0600)

Density 1800)

Density 0600)

Density 1800)

Density 0600)

Density 1800)

Density (1800-0600)

Density M

C) 0_-I. /100 M /100 f m3 /100 M3 /100m, /1O0m' /l00m, /100m, /1O0M3 /1O0M3 /100 M3 /100 M3 /100 m, Jul- Alewife Egg 0 0 0 0 0 0 0 0 0 0 0 0 05 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 I-

-C larvae Post-yolk-sac 0.9 9.9 2.5 5.9 2 11.4 0 1.1 0 4 1.9 25.1 larvae Common Post-yolk-sac 0 0 0 *0 0 0 0. 0 0 0 0.9 0 CO carp larvae Cyprinidae Unidentified 0 0 0 0 0 0 0 0 0 0 0 0 Yolk-sac 0 0 0 0.7 0 1.9 0 1.1. 0 0 0.9 1 -I larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 2.8 1 larvae Round Post-yolk-sac 0 0 0 0 0 0 0 0 0: 0 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 1.9 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 z perch larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 0 larvae Total 0.9 9.9 2.5 6.6 2 15.3 0 2.2 0 4 6.6 27.2 (continued)

CL 0,.

00

Appendix Table L. (Continued)

C) 0 Station 0

01 Experimental, 30-40 Experimental, surface -

feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline o

0) Diel Diel Diel Diel Diel Diel CO CI)

Day Night Day Night Day Night Day Night Day Night Day rn.

(0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night a) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) (1800-0600)

Density Density3 Density Density Density3 Density Density Density10 DensityM Density Density Density3

_1_/100m' /100 m /100lm /100m' /100m /10m3 /100 M3 /100 m 0 M3 m 3100m /100 m3 /100 m CO) rr

.- 1, Aug- Alewife Egg 0 0 0 0 0 0 0 0 0 0 0 0 05 Yolk-sac 0 0 0 0 0 0 0 0. 0 0 0 .0 4x larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Common Post-yolk-sac 0 0 0 0 0 0 .0 0 0 0 0 0 rn carp larvae Cyprinidae Unidentified 0 0 0 0 0 0 0 0 0 0 0 0 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 CO larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae II Round Post-yolk-sac 0. 0 0 0 0 0 0 0 0 0 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0. 0 0 0 whitefish larvae Post-yolk-sac. 0 0 0 0 0 0 0 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 0 perch larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Total 0 0 0 0 0 0 0 0 0 0 0 0 (continued) 0 C*)

Appendix Table L. (Continued)

CD 0

r0) Station 0

Experimental, 30-40 Experimental, surface -

0 feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline 0

Diel Diel Diel Diel Diel Diel Day Night Day Night Day Night Day Night Day Night Day (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night CO, 03 0

1800) 0600) 1800) 0600) 1 1800) 0600) 1800) 0600) 1800) 0600) 1800) (1800-0600) w Density Density Density Density Density Density Density Density Density Density Density Density 3

100m /100 m 3 10m /100 m 3

/100 m 3

/100 m 3

/100 m 3

/100 m, /100m3 /100m 3 /100Dm 3 /100 m, 0

Sep Alewife Egg 0 0 0 0 0 0 0 0 0 0 0 0 05 Yolk-sac 0 0 0 0 0 0 .0 0 0 0 0 0 larvae rrn Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 S.-

larvae Common Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 carp larvae Cyprinidae Unidentified 0 0 0 0 0 0 0 0 0 0 0 0 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 co larvae C*

Post-yolk-sac 0 0 0 0 0 0 0 0 0 .0 0 0 larvae Round Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0 0 0 .0 0 0 perch larvae

.5' Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Total . 0 0 0 0 0 0 0 0 0 0 0 0 Q.

CD (continued) w a,

0 CD p

K3Appendix Table L. (Continued) 4c53 C) Station 0

0 Experimental, 30-40 Experimental, surface -

0) feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline ,a Diel Diel Diel Diel Diel Diel Day Night Day Night Day Night Day Night Day Night Day CD (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) (1800-0600)

Cn I.M Density Density Density3 Density Density Density3 Density3 Density Density3 Density Density Density

/100 m3 /100 m3 /100 m /100 m3 /100 m3 /100 m /100 m /100 m3 /100 m /100 m3 /100 m3 /100 m3 CD

-4 Oct- Alewife Egg 0 0 0 0 0 0 0 0 0 0 0 0 05 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 to larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larivae In, Common Post-yolk-sac 0 0 0 0 0 0 0 -0 0 0 0 0 carp larvae Cyprinidae Unidentified* 0 0 0 0 0 0 0 0 0 0 0 0 "I Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 .0 larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Round Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 goby larvae 00 Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 perch larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Total 0 0 0 0 0 0 0 0 0 0 0 0 0CL M) (continued) lb

Appendix Table L. (Continued) 0 Station - XA 03 Experimental, 30-40 Experimental, surface -

feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline o Diel Diel Diel IDiel Diel Diel Mo Day Night Day Night Day Night Day Night Day Night Day (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night

-n 1800) '0600) 1800) 0600) 1800) 0600) 1800). 0600) 1800) 0600) 1800) (1800-0600)

EDensity3 Density3 Density3 I Density3 Density Density Density Density -Density3 Density3 Density3 Density 3

/100mu 1100m 1100m /100 m /100 /10 /100 M / /100 M 100m /100 m /100 m /100m 0

Nov- Alewife Egg 0 0 0 0 0 0 0 0 0 0 0 0 CD 05 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 zn-larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Common Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 CO carp larvae Cyprinidac Unidentified 0 0 0 0 0 0 0 0 0 0 0 0 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 C,,

larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Round Post-yolk-sac 0 0 0 0 0 0 0 0. 0 0 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 0. 0 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 .'0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 perch larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Total 0 0 0 0 0 0 0 0 0 0 0 0 0 (continued) 0o Co 05 C,

Appendix Table L. (Continued) t3o C) 0 Station Experimental, 30-40 Experimental, surface -

feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline Diel Diel Diel Diel Diel Diel CD Day Night Day Night Day Night Day Night Day Night Day (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) (1800-0600)

C.

Density Density Density Density Density Density Density Density Density Density Density Density 3

/100 m /100 m 3 /100 m 3

/100m 3 /100 M 3

/100 m3 /100 m 3

/100 m 3 /100 m 3

/100 m 3 /100 m 3 /100 m 3 Apr- Alewife Egg 0 0 0 0 0 0 0 0 0 0 0 0 06 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 1.8 M larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Common Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 carp larvae In Cyprinidae Unidentified 0 0 0 0 0 0 0 0 0. 0 0 0 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Round Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 2.7 whitefish larvae Post-yolk-sac 0 0 0 0 0 0 0 0 1.9 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 .0 0 0 0 0 0 0 0

0) perch larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0

- --o.larvae ,.... . . - , . ,

Total 0 0 0 0 0 0 0 1.9 0 0 4.4 0

(continued)

Appendix Table L. (Continued) 41 CM 0

0 0

Station CO)

Experimental, 30-40 Experimental, surface -

co3 feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline Diel Diel Diel Diel Diel Diel Day Night Day Night Day Night Day Night Day Night Day 0

(0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night bi, 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) (1800-0600) a CD Density3 Density Density3 Density3 Density3 Density3 Density3 Density Density3 Density3 Density3 Density3

/100 m /100 m3 /100 m /100 m /100 m /100 m /100 m /100 m3 /100 m /100 m /100 m /100 m C,,

C>

4 May Alewife Egg 0 0 0 0 0 0 0 0 0 0 0 0

-06 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 C'n larvae 74" Common Post-yolk-sac 0 0 0 0 0 0 0 0 0 "0 0 0 carp larvae I ZI Cyprinidae Unidentified 0 0 0 0. 0 0 0 0 0 0 0 0 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Round Post-yolk-sac 0 0 0 0 0 0 0 -0 0 0. 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 perch larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 1 larvae 0 Total 0 0 0 0 0 0 0 0 0 0 0 1 (continued)

Q.

0 CD

'5'

C)

Appendix Table L. (Continued) 0 0

0 Station Experimental, 30-40 Experimental, surface -

feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline C.

CD Diel Diel Diel Diel Diel Diel a) Day Night Day Night Day Night Day Night Day Night Day CO (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night a0~ 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) .1800) (1800-0600)

Density Density Density Density Density Density Density Density Density Density Density Density 5.4 /100 M3 /100 m3 /100 /100 Mm /100 M3 /100 Mm /100mn mm /10 ' /100M3 /100M, /1m0 M3 rn

/100 m3 Jun- Alewife Egg 0 0 0 0 0 0 0 0 0 0 0 13.8 06 Yolk-sac 0 0 0 0 0 3.3 0 4.6 0 6.6 0 0 V; larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 1.1 0 larvae X Common Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 carp larvae Cyprinidae Unidentified 0 0 0 0 0 0 0 0 0 0 0 0 M q.

Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 50.3 CO larvae Q,

-I Post-yolk-sac 0 0 0 0 0 0 1.8 0 0 1.3 1.1 34.9 larvae Round Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0

,> goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 0~ larvae I Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0 0 5.3 0 0 (0 perch larvae Post-yolk-sac 0 0 0.5 0 0 0 0 0 0 1.3 0 0 larvae Total 0 0 0.5 0 0 3.3 1.8 4.6 0 14.5 2.1 99 (continued)

C')

0

'5' p

Appendix Table L. (Continued) 0~ Station

• 0 CA) 0' Experimental, 30-40 Experimental, surface -

C70 feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline

0) Diel Diel Diel Diel Diel Diel CD Day Night Day Night Day Night Day Night Day Night Day 2i (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night a,

1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) (1800-0600) 0 Density Density Density Density Density Density Density Density Density Density Density Density 0

/100 m 3 3 Ci,

/100 m3 /100 m3 /100 m, /100 m /100 m3 /100 m3 /100 m, /100 m3 /100 m3 /100 m 3 /100 m, C) Jul- Alewife Egg 0 0 0 0 0 0 0 .0 0 0 0 0 4 06 Yolk-sac 0 1.9 2.7 2 0 2 6.6 1.8 0 4.6 1.7 0 ftn larvae Post-yolk-sac 0 1.9 0.5 6.1 3.8 0 7.9 17.9 0 9.2 0.9 57.8 Tn larvae Common Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 PO, carp larvae Cyprinidae Unidentified 0 0 0 0 0 0 0 0 0 0 0 0 Yolk-sac 0 0 0 0 0 2 0 1.8 0 0 0 3 W, larvae Post-yolk-sac 0 0 0 0 0 0 0 1.8 0 0 0 6 larvae Round Post-yolk-sac 0 1.9 0 0.5 0 14.1 0 10.7 0 0 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 0 0. 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0, 0 0 0 0 0 perch larvae Post-yolk-sac 0 0 0 0 0 0 0 .0 0 0 0 0 larvae Total 0 5.6 3.3 8.6 3.8 18.1 14.6 34 0 13.8 2.6 66.7 (continued) 0 Qo

• 5n pn

Appendix Table L. (Continued)

W1 C) 0 0 Station C.,

Experimental, 30-40 Experimental, surface -

feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline 1o Diel Diel Diel Diel Diel Diel rfl C6)

Day Night Day Night Day Night Day Night Day Night Day (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night 01 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) (1800-0600)

Density Density Density Density Density Density Density Density Density Density Density Density I-3 3 3 Cn

/100 m /100 m3 /100 m3 /100 m3 /100 m /100 m3 /100 M3 /100 m3 /100 m3 /100m /100m' /100 m3 Aug- Alewife Egg 0 0 0 0 0 0 0 0 0 0 0 0 06 Yolk-sac 0 0 0 0 0 0 0 *0 0 0 0 0 94 larvae Post-yolk-sac 0 0 0 0 0

'n 0 0 0 0 0 0 0.9 larvae Common Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 Vi carp larvae Cyprinidae Unidentified 0 0 0 0 0 0 0 0 0 0 0 0 Yolk-sac 0 0 0 0 0 0 0 0 0 CO) 0 0 0 Co larvae (Z Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Round Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 0 perch larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Total 0 0 0 0 0 0 0 0 0 0 0 0.9 CD (continued) 0)

Appendix Table L. (Continued) cn PQ 0

0 Station Experimental, 30-40 Experimental, surface -

0, feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline 0,

Diel Diel Diel Diel Diel Diel X C,

Day Night Day Night Day Night Day Night Day. Night Day (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night Co CD "1, 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) (1800-0600) i.

Density Density Density Density Density Density Density Density Density Density Density3 Density

/100 m, /100 m3 /100 m3 /100 m3 /100 m, /100 m3 /100 m3 /100 m /100 m3 /100 m3 /100 m /100 m3 0

Sep- Alewife Egg 0 0 0 0 0 0 0 0 0 M

0 0 0 0 06 Yolk-sac 0 0 0 0- 0 0 0 0 0 0 0 0 larvae hi:

Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 rri larvae Common Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 carp larvae Cyprinidae Unidentified 0 0 0 0 0 0 0 0 0 0 0 0 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 -*1 larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Round Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0 0 0 -* 0 0 (J3 0 perch larvae Post-yolk-sac 0 0 0 0. 0 0 0 0 0 0 0 0 larvae Total 0 0. 0 0 0 0 0 0 0 0 1 0 0 (continued) o 0

Appendix Table L. (Continued) 01 r')

0 Station 0

,w Experimental, 30-40 Experimental, surface -

0a feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline Diel Diel Diel Diel _ _ Diel Diel co Day Night Day Night Day Night Day Night Day Night Day rn CD (0600- (1800- (0600- (1800- (0600- (1800- (0600- - (1800- (0600- (1800- (0600- Night

-n 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) 0600) 1800) (1800-0600)

Density Density Density Density Density Density Density Density Density Density Density Density 3

/100m 3 3 DCI 0~ /100 m /100m /100 m 3 /100 m 3

/100 m3 /100 m 3

/100 m 3 /100 m 3

/100 m3 /100 m3 /100 m 3 C) 0-'" Oct- Alewife .Egg 0 0 0 -0 0 0 0 0 0 0 0 0 06 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 CO) 4 larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 m larvae Common' Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 carp larvae P, Cyprinidae Unidentified 0 0 0 0 0 0 0 0 0 0 0 0 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 -I larvae I Round Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 0 perch larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 a) larvae Total 0 0 0 0 0 0 0 0 0 0 0 0 a) (continued) a,

'A 0

a) p

Appendix Table L. (Continued) 4n 0 Station 0

Experimental, 30-40 Experimental, surface -

0- feet 20 feet Intake, 22 feet Intake, 11 feet Intake, surface Shoreline c,a co Diel Diel Diel Diel Diel Diel Day Night Day Night Day Night Day Night Day Night Day

3 (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- (1800- (0600- Night CD 1800) 0600) 1800) 0600) 2i 1800) 0600) 1800) 0600) 1800) 0600) 1800) (1800-0600)

Density3 Density3 Density3 Density Density Density Density Density Density 'Density Density Density

0) /100 m /100m /100m 1100 m3 /100 m3 /100 m3 /100 m3 /100 m3 /100 m 3 /100m 3 /100 m 3 /100 m3 CO)

• 0D Nov- Alewife Eg0 0 0 0 0 0 0 0 0 0 0 0 0 06 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Post-yolk-sac 0 0 .0 0 0 0 0 0 0 0 0 .0 larvae

_nj Common Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 carp larvae Cyprinidae Unidentified 0 0 0 0 0 0 0 0 0 "I 0 0 0 Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Round Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 goby larvae Round Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 whitefish larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Slimy Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 sculpin larvae Yellow Yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 perch larvae Post-yolk-sac 0 0 0 0 0 0 0 0 0 0 0 0 larvae Total 0 0 0 0 0 .0 0 0 0 0 0 0 0

9)

316(b) PHASE It BASELINE FISH E & I STUDY Appendix Table M. Mean Catch per Unit Effort (fish per hour) of Fish Captured in Gill Nets in the Vicinity of Cook Nuclear Plant, June through November 2005 and April through November 2006.

Station Intake 40 ft Diel Diel Day (0600- Night (1800- Day (0600- Night (1800-1800) 0600) 1800) 0600)

CPUE CPUE CPUE CPUE Jun-05 Alewife 0.00 0.00 0.00 0.00 Bloater 0.00 0.00 0.00 0.00 Channel catfish 0.00 0.00 0.00 0.00 Common carp 0.00 0.00 - 0.00 0.00 Freshwater drum 0.00 0.00 0.00 0.00 Gizzard shad 0.00. 0.00 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.00 0.00 0.00 Lake trout 0.00 0.00 0.00 0.49 Lake whitefish 0.00. 0.00 0.00 0.24 Longnose sucker 0.00 0.24 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 Spottail shiner 0.00 5.76 0.00 0.00 Walleye 0.00 0.00 0.00 0.00 White sucker 0.89 0.24 0.00 0.00 Yellow perch 9.33 0.00 0.00 0.00 Total 10.22 6.24 0.00 0.73 Jul-05 Alewife 0.00 0.00 0.00 0.00 Bloater 0.00 0.00 0.00 0.00 Channel catfish .0.00 0.00 0.00 0.50 Common carp 0.00 0.00 0.00 0.00 Freshwater drum 0.00 0.00 0.00 0.00 Gizzard shad 0.00 0.00 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.00 0.00 0.00 Lake trout 0.00 0.00 0.00 0.00 Lake whitefish 0.00 0.00 0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.25 Spottail shiner 0.00 4.34 0.00 3.00 Walleye 0.00 0.24 0.00 0.00 White sucker 0.00 0.00 0.00 0.00 Yellow perch 0.00 4.10 4.75 0.25 Total 0.00 8.67 4.75 4.00 Aug-05 Alewife 0.00 0.00 '0.00 0.00 Bloater 0.00 0.00 0.00 0.24 Channel catfish 0.00 0.00 0.00 0.00 -

Common carp 0.00 '0.00 0.00 0.00 Freshwater drum 0.00 0.00 0.00 0.00 Gizzard shad 0.00 0.00 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.00 0.00 0.00 Lake trout 0.00 0.71 0.00 0.24 Lake whitefish 0.00 0.00 0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 Rainbow smelt 1.18 0.00 0.75 0.00 Round goby 0.00 0.00 0.00 0.00 (continued) 20452 Cook 316b Baseline Final.doc 10/4/07 A-58 Normandeau Associates, Inc.

3 16(b) PHASE I/ BASELINE FISH E & I STUDY Appendix Table M. (Continued)

Station Intake 40 ft Diel Diel Day (0600- Night (1800- Day (0600- Night (1800-1800) 0600) 1800) 0600)

CPUE CPUE CPUE CPUE Aug-05 Spottail shiner 0.00 0.95 0.00 0.00 (Cont'd) Walleye 0.00 0.24 0.00 0.00 White sucker 0.24 0.00 0.00 0.00 Yellow perch 0.24 0.24 1.00 0.00 Total 1.65 2.13 1.76 0.47 Sep-05 Alewife 0.00 0.00 0.00 0.00 Bloater 0.00 0.00 0.00 0.00 Channel catfish 0.00 0.00 0.00 0.00 Common carp 0.00 0.24 0.00 0.00 Freshwater drum 0.00 0.24 0.00 0.00 Gizzard shad 0.23 0.24 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.00 .0.00 0.00 Lake trout 0.00 0.00 0.00 0.00 Lake whitefish 0.00 0.00 0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 Spottail shiner 0.00 1.71 0.00 0.00 Walleye 0.00 0.00 0.00 0.00 White sucker 0.00 0.00 0.00 0.00 Yellow perch 4.42 0.24 0.00 0.00 Total 4.65 2.69 0.00 0.00 Oct-05 Alewife 0.00 0.00 1.74 0.00 Bloater 0.00 0.00 0.50 0.00 Channel catfish 0.00 0.00 0.00 0.00 Common carp 0.00 0.25 0.00 0.00 Freshwater drum 0.00 0.74 0.25 0.00 Gizzard shad 0.00 0.00 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.00 0.00 0.00 Lake trout 0.00 0.00 0.00 0.00 Lake whitefish 0.00 0.00 0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 Spottail shiner .0.00 0.98 0.00 0.00 Walleye 0.00 0.00 0.00 0.00 White sucker 0.00 0.00 0.00 0.00 Yellow perch 0.00 0.00 0.00 0.00 Total 0.00 1.97 2.48 0.00 Nov-05 Alewife 0.00 0.00 0.00 0.00 Bloater 0.00 0.00 0.00 0.00 Channel catfish 0.00 0.00 0.00 0.00 Common carp 0.00 0.00 0.00 0.00 Freshwater drum 0.00 0.00 0.00 0.00 Gizzard shad 0.00 0.00 0.00 0.00 Golden redhorse 0.00 0.24 0.00 0.00 Lake sturgeon 0.00 0.00 0.00 0.00 Lake trout 0.00 0.00 0.00 0.00 Lake whitefish 0.00 0.00 -.0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 (continued) 20452 Cook 316b Baseline Final.doc 10/4/07 A-59 NormandeauAssociates, Inc.

316(b) PHASE I/ BASELINE FISH E & I STUDY Appendix Table M. (Continued)

Station Intake 40 ft Diel Diel Day (0600- Night (1800- Day (0600- Night (1800-1800) 0600) 1800) 0600)

CPUE CPUE CPUE CPUE Nov-05 Rainbow smelt 0.47 0.00 0.00 0.00 (Cont'd) Round goby 0.00 0.49 0.00 0.00 Spottail shiner 1.88 0.73 0.50 0.00 Walleye 0.00 0.00 0.25 0.00 White sucker 0.00 0.00 0.00 0.00 Yellow perch 0.00 0.00 0.00 0.00 Total 2.34 1.47 0.74 0.00 Apr-06 Alewife 0.00 0.00 0.00 0.00 Bloater 0.00 0.00 0.00 0.00 Channel catfish 0.00 0.00 0.00 0.00 Common carp 0.00 0.00 0.00 0.00 Freshwater drum 0.00 0.00 0.00 0.00 Gizzard shad 0.00 0.23 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.00 0.00 0.00 Lake trout 0.00 0.00. 0.22 0.00 Lake whitefish 0.00 0.00 0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 Spottail shiner 0. 00 0.23 0.00 0.00 Walleye 0.00 0.00 0.00 0.00 White sucker . 0.00 0.00 0.00 0.00 Yellow perch 0.00 0.00 0.00 0.00 Total 0.00 0.46 0.22 0.00 May-06 Alewife 0.00 0.00 0.00 0.00 Bloater 0.00 0.00 0.00 0.00 Channel catfish 0.00 0.00 0.00 0.00 Common carp 0.00 0.00 0.00 0.00 Freshwater drum 0.00 0.00 0.00 0.00 Gizzard shad 0.00 0.00 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.00 0.00 0.00 Lake trout 0.00 0.00 0.00 0.00 Lake whitefish 0.00 0.00 0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 Spottail shiner 0.00 0.50 0.00 0.00 Walleye 0.00 0.00 0.00 0.00.

White sucker 0.00 0.00 0.00 0.00 Yellow perch 0.00 0.00 0.00 0.00 Total 0.00 0.50 0.00 0.00 Jun-06 Alewife 0.26 0.00 0.00 0.00 Bloater 0.00 0.00 0.00 0.00 Channel catfish 0.00 0.00 0.00 0.00 Common carp 0.00 0.00 0.00 0.00 Freshwater drum' 0.00 0.00 0.00 0.00 Gizzard shad 0.00 0.00 0.00 0.00 Golden redhorse 0.00 0.00 0.00 .0.00

-Lake sturgeon 0.00 0.00 0.00 0.00 Lake trout 0.00 0.00 0.00 0.00 (continued) 20452 Cook 316b Baseline Final.doc 10/4/07 A-60 Normandeau Associates, Inc.

3 16(b) PHASE HI BASELINE FISH E & I STUDY Appendix Table M. (Continued)

Station Intake 40 ft Diel Diel Day (0600- Night (1800- Day (0600- Night (1800-1800) 0600) 1800) 0600)

CPUE CPUE CPUE CPUE Jun-06 Lake whitefish 0.00 0.00 0.00 0.00 (Cont'd) Longnose sucker 0.00 1.20 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 Spottail shiner 0.00 0.72 0.00. 0.00 Walleye 0.00 0.00 0.00 0.00 White sucker 0.00 0.00 0.00 0.00 Yellow perch 0.00 0.00 0.00 0.00 Total 0.26 1.92 0.00 0.00 Jul-06 Alewife 0.00 0.00 0.24 0.00 Bloater 0.00 0.00 0.00 0.00 Channel catfish 0.00 0.00 0.00 0.00 Common carp 0.00 0.00 0.00 0.00 Freshwater drum 0.00 0.00 0.00 0.00 Gizzard shad 0.00 0.00 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.00 0.00 0.00 Lake trout 0.00 0.17 0.00 0.00 Lake whitefish 0.00 0.00 0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.17 0.00 0.00 Spottail shiner 0.48 0.67 1.42 0.00 Walleye 0.00 0.00 0.00 0.00 White sucker 0.00' 0.00 0.47 0.00 Yellow perch 0.73 0.00 0.47 0.00 Total 1.21 1.00 2.60 0.00 Aug-06 Alewife 0.00 0.00 0.00 0.00 Bloater 0.00 0.00 0.00 0.00 Channel catfish 0.00 0.00 0.00 0.00 Common carp 0.00 0.00 0.00 0.00 Freshwater drum 0.00 0.00 0.00 0.00 Gizzard shad 0.00 0.00 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.50 0.00 0.00 Lake trout 0.00 0.00 0.00 0.00 Lake whitefish 0.00 0.00 0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 Spottail shiner 0.00 0.00 . 0.00 0.93 Walleye 0.00 0.25 0.00 0.00 White sucker 0.00 0.00 0.00 0.00 Yellow perch 0.25 0.00

• 0.75 0.00 Total 0.25 0.75 0.75 0.93 Sep-06 Alewife 0.00 0.00 0.00 0.00 Bloater 0.00 0.00 0.00 0.00 Channel catfish 0.00 0.00 0.00 0.00 Common carp 0.00 0.00 0.00 0.00 Freshwater drum 0.00 0.24 0.00 0.00 Gizzard shad 0.00 0.00 . 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 (continued) 20452 Cook 316b Baseline Final.doc 10/4/07 A-61 Normandeau Associates, Inc.

316(b) PHASE I1BASELINE FISH E & I STUDY Appendix Table M. (Continued)

Station Intake 40 ft Diel Diel Day (0600- Night (1800- Day (0600- Night (1800-1800) 0600) 1800) 0600)

CPUE CPUE CPUE CPUE Sep-06 Lake sturgeon 0.00 0.00 0.00 0.00 (Cont'd) Lake trout 0.00 0.00 0.00 0.00.

Lake whitefish 0.00 0.00 0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 Spottail shiner 0.00 0.72 0.00 0.24 Walleye 0.00 0.00 0.00 0.00 White sucker 0.00 0.00 0.00 0.00 Yellow perch 0.00 0.72 0.00 0.24 Total 0.00 1.69 0.00 0.47 Oct-06 Alewife 0.00 0.00 0.00 0.00

'Bloater 0.00 0.26 0.00 0.00 Channel catfish 0.25 0.26 0.00 0.00 Common carp 0.00 0.00 0.00 0.00 Freshwater drum 0.00 0.79 0.00 0.50 Gizzard shad 0.25 0.26 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 . 0.00 0.00 0.00 Lake trout 0.00 0.00 0.00 0.00 Lake whitefish 0.00 0.00 0.00 0.00 Longnose sucker 0.00 0.00 0.00 0.00 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 Spottail shiner 0.00 2.64 0.00 0.25 Walleye 0.00 0.00 0.00 0.00 White sucker 0.00 0.00 0.00 0.00 Yellow perch 0.00 2.11 0.00 1.00 Total 0.50 6.34 0.00 1.76 Nov-06 Alewife 0.00 0.00 0.00 0.00 Bloater 0.25 0.00 0.00 0.24 Channel catfish 0.00 0.00 0.00 0.00 Common carp 0.00 0.00 0.00 0.00 Freshwater drum 0.00 0.00 0.00 0.00 Gizzard shad 0.00 0.00 0.00 0.00 Golden redhorse 0.00 0.00 0.00 0.00 Lake sturgeon 0.00 0.00 0.00 0.00 Lake trout 0.00 0.25 0.00 0.00 Lake whitefish 0.00 0.25 0.00 0.24 Longnose sucker 0.00 0.00 0.00 0.24 Rainbow smelt 0.00 0.00 0.00 0.00 Round goby 0.00 0.00 0.00 0.00 Spottail shiner 0.00 0.25 0.00 0.24 Walleye 0.00 0.00 0.00 0.24 White sucker 0.00 0.00 0.00 0.00 Yellow perch 0.00 0.00 0.00 0.24 Total 0.25 0.74 0.00 1.45 20452 Cook 316b Baseline Final.doc 10/4/07 A-62 Normandeau Associates, Inc.

316(b) PHASE II BASELINE FISH E & I STUDY Appendix Table N. Mean Catch per Unit Effort (fish per trawl) of Fish Captured in the Otter Trawl in the Vicinity of Cook Nuclear Plant, June through November 2005 and April through November 2006.

Station Shore Intake (22 ft) Experimental (40 ft)

Diel Diel Diel Day Night Day Night Day Night (0600-1800) (1800-0600) (0600-1800) (1800-0600) (0600-1800) (1800-0600)

CPUE PPEU CPUE CPUE CPUE Jun-05 Alewife 1.0 3.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 Lake whitefish 0.0 1.0 0.0 0.0 Rainbow smelt 0.0 0.0 0.0 0.0 Round goby 0.0 4.0 0.0 0.0 Slimy sculpin 0.0 0.0 0.0 0.0 Spottail shiner 3.0 14.0 0.0 0.0 White sucker 0.0 0.0 0.0 0.0 Yellow perch 5.0 5.0 1.0 1.0 Total 9.0 27.0 1.0 1.0 Jul-05 Alewife 0.0 0.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0U0 0.0 Rainbow smelt 0.0 0.0 0.0 0.0 Round goby 0.0 0.0 0.0 0.0 Slimy sculpin 0.0 0.0 0.0 0.0 Spottail shiner 0.0 1.0 0.0 0.0 White sucker 0.0 0.0 0.0 0.0 Yellow perch 0.0 0.0 2.0 0.0 Total 0.0 1.0 2.0 0.0

.Aug-05 Alewife 0.0 3.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 Rainbow smelt 0.0 0.0 0.0 0.0 Round goby 0.0 0.0 1.0 3.0 Slimy sculpin 0.0 0.0 0.0 0.0 Spottail shiner 0.0 4.0 0.0 0.0 White sucker 0.0 0.0 0.0 0.0 Yellow perch 1.0 0.0 0.0 0.0 Total 1.0 7.0 1.0 3.0 Sep-05 Alewife 0.0 0.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 Lake whitefish 0.0 1.0 0.0 0.0 Rainbow smelt 0.0 0.0 0.0 0.0 Round goby 0.0 0.0 4.0 0.0 Slimy sculpin 0.0 0.0 0.0 0.0 Spottail shiner 0.0 0.0 0.0 0.0 White sucker 0.0 0.0 0.0 0.0 Yellow perch 99.0 0.0 0.0 0.0 Total 99.0 1.0 4.0 0.0 (continued) 20452 Cook 316b Baseline Final.doc 10/4/07 A-63 Norma'ndeauAssociates, Inc.

3 16(b) PHASE II BASELINE FISH E & I STUDY Appendix Table N. (Continued)

Station Shore Intake (22 ft) Experimental (40 ft)

Diel Diel Diel Day Night Day Night Day Night (0600-1800) (1800-0600) (0600-1800) (1800-0600) (0600-1800) (1800-0600)

CPUE CPUE CPUE CPUE CPUE CPUE Oct-05 Alewife 0.0 1.0 0.0 1.0 0.0 0.0 Bloater 0.0 3.0 0.0 3.0 0.0 9.0 Gizzard shad 0.0 17.0 0.0 3.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 0.0 0.0 Rainbow smelt 0.0 0.0 0.0 0.0 0.0 0.0 Round goby 0.0 0.0 0.0 0.0 0.0 1.0 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner 1.0 0.0 0.0 2.0 0.0 0.0 White sucker 0.0 0.0 0.0 0.0 0.0 0.0 Yellow perch 0.0 2.0 0.0 11.0 6.0 16.0 Total 1.0 23.0 0.0 20.0 6.0 26.0 Nov-05 Alewife 0.0 0.0 0.0 0.0 0.0 0.0 Bloater 0.0 -0.0 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 0.0 0.0 Rainbow smelt 0.0 0.0 0.0 0.0 0.0 0.0 Round goby 0.0 0.0 0.0 0.0 0.0 0.0 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner 0.0 0.0 0.0 1.0 0.0 0.0 White sucker 0.0 0.0 0.0 0.0 0.0 0.0 Yellow perch 0.0 0.0 4.0 0.0 0.0 0.0 Total 0.0 0.0 4.0 1.0 0.0 0.0 Apr-06 Alewife 0.0 0.0 0.0 0.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 0.0 0.0 Rainbow smelt 0.0 0.0 0.0 0.0 0.0 1.0 Round goby 0.0 0.0 0.0 0.0 2.0 0.0 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 1.0 Spottail shiner 0.0 1.0 0.0 0.0 0.0 2.0 White sucker 0.0 0.0 0.0 0.0 0.0 0.0 Yellow perch 1.0 0.0 3.0 0.0 3.0 2.0 Total 1.0 1.0 3.0 0.0 5.0 6.0 /

May-06 Alewife 0.0 0.0 0.0 0.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 0.0 4.0 Rainbow smelt 0.0 0.0 0.0 0.0 1.0 1.0 Round goby 0.0 1.0 0.0 1.0 2.0 3.0 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner 0.0 5.0 0.0 1.0 0.0 1.0 White sucker 0.0 0.0 0.0 0.0 0.0 0.0 Yellow perch 1.0 1.0 5.0 9.0 7.0 3.0 Total 1.0 7.0 5.0 11.0 10.0 12.0 (continued) 20452 Cook 316b Baseline Final.doc 10/4/07 A-64 NormandeauAssociates, Inc.

3 16(b) PHASE I/ BASELINE FISH E & I STUDY Appendix Table N. (Continued)

Station Shore Intake (22 ft) Experimental (40 ft)

Diel Diel Diel Day Night Day Night Day Night (0600-1800) (1800-0600) (0600-1800) (1800-0600) (0600-1800) (1800-0600)

CPUE CPTEPUE CPUE CPUE CPUE Jun-06 Alewife 0.0 0.0 0.0 0.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 0.0 2.0 Rainbow smelt 0.0 0.0 0.0 0.0 0.0 0.0 Round goby 0.0 0.0 0.0 0.0 6.0 3.0 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner 0.0 0.0 0.0 3.0 0.0 1.0 White sucker 0.0 0.0 1.0 0.0 0.0 0.0 Yellow perch' 2.0 0.0 57.0 10.0 0.0 1.0' Total 2.0 0.0 58.0 13.0 6.0 7.0 Jul-06 Alewife 0.0 0.0 0.0 3.0 1.0 4.0 Bloater 0.0 0.0 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 0.0 5.0 Rainbow smelt 0.0 0.0 0.0 0.0 0.0 0.0 Round goby 0.0 0.0 0.0 1.0 5.0 11.0 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner 0.0 0.0 0.0 2.0 2.0 2.0 White sucker 0.0 0.0 0.0 0.0 0.0 0.0 Yellow perch 50.0 0.0 105.0 7.0 3.0 1.0 Total 50.0 0.0 105.0 13.0 11.0 23.0 Aug-06 Alewife 0.0 0.0 0.0 0.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 0.0 0.0 Rainbow smelt 0.0 0.0 0.0 0.0 0.0 0.0 Round goby 0.0 0.0 0.0 0.0 3.0 0.0 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner, 0.0 1.0 0.0 0.0 0.0 0.0 White sucker 0.0 0.0 0.0 0.0 0.0 0.0 Yellow perch 0.0 0.0 0.0 2.0 0.0 1.0 Total 0.0 1.0 0.0 2.0 3.0 1.0 Sep-06 Alewife 0.0 0.0 0.0 0.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 0.0 0.0 Rainbow smelt 0.0 0.0 0.0 0.0 0.0 0.0 Round goby 0.0 0.0 0.0 0.0 1.0 0.0 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner 0.0 0.0 0.0 0.0 0.0 0.0 White sucker 0.0 0.0 0.0 0.0 0.0 0.0 Yellow perch 0.0 0;0 1.0 0.0 2.0 0.0 Total 0.0 0.0 1.0 0.0 3.0 0.0 (continued) 20452 Cook 316b Baseline Final.doc 10/4/07 A-65 NormandeauAssociates, Inc.

3 16(b) PHASE II BASELINE FISH E & I STUDY Appendix Table N. (Continued)

Station Shore Intake (22 ft) Experimental (40 ft)

Die g Die g Diel Day Night Day Night Day Night (0600-1800) (1800-0600) (0600-1800) (1800-0600) (0600-1800) (1800-0600)

CPU CPUE CPUE PUE CPUE CPUE Oct-06 Alewife 0.0 0.0 0.0 0.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 "0.0 0.0 Rainbow smelt 0.0 0.0 0.0 0.0 0.0 0.0 Round goby 0.0 0.0 0.0 0.0 0.0 0.0 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner 1.0 0.0 0.0 0.0 0.0 0.0 White sucker 0.0 0.0 0.0 0.0 0.0 0.0 Yellow perch 0.0 0.0 1.0 0.0 0.0 1.0 Total 1.0 0.0 1.0 0.0 0.0 1.0 Nov-06 Alewife 0.0 0.0 0.0 0.0 0.0 0.0 Bloater 0.0 0.0 0.0 0.0 0.0 0.0 Gizzard shad 0.0 0.0 0.0 0.0 0.0 0.0 Lake whitefish 0.0 0.0 0.0 0.0 0.0 0.0 Rainbow smelt 0.0 0.0 0.0 0.0 0.0 0.0 Round goby ' 0.0 0.0 0.0 0.0 0.0 0.0 Slimy sculpin 0.0 0.0 0.0 0.0 0.0 0.0 Spottail shiner 0.0 1.0 0.0 1.0 0.0 8.0 White sucker 0.0 0.0 0.0 0.0 0.0 0.0 Yellow perch 0.0 1.0 0.0 1.0 2.0 3.0 Total 0.0 2.0 0.0 2.0 2.0 11.0 20452 Cook 316b Baseline Final.doc 10/4/07 A-66 Normandeau Associates, Inc.

316(b) PHASE II BASELINE FISH E & I STUDY Appendix Table 0. Mean Catch per Unit Effort (fish per haul) of Fish Captured in the Seine in the Vicinity of Cook Nuclear Plant, June through November 2005 and April through November 2006.

Diel Night (1800-Day (0600-1800) 0600)

CPUE CPUE Aug-05 Alewife 2.5 17.0 Bloater 1.0 2.0 Bluegill 0.0 0.0 Brook silverside 0.0 0.0 Chinook 0.0 0.0 Coho 0.0 0.0 Eastern banded killifish 1.0 0.0 Gizzard shad 0.0 0.0 Lake trout 0.0 0.0 Longnose sucker 0.0 0.0 Muskellunge 0.0 0.0 Rainbow smelt 0.0 0.0 Round goby 0.5 0.5 Shorthead redhorse 0.0 0.0 Spottail shiner 1.0 30.0 Steelhead 0.0 0.0 Walleye 0.0 0.0 White sucker 0.0 0.0 Yellow perch 545.5 68.0 Total 551.5 117.5 Sep-05 Alewife 0.0 0.0 Bloater 0.5 0.0 Bluegill 0.0 0.0 Brook silverside 0.0 0.0 Chinook 0.0 0.0 Coho 0.0 0.0 Eastern banded killifish 0.5 0.0 Gizzard shad 0.0 0.5 Lake trout 0.0 0.0 Longnose sucker 0.0 0.0 Muskellunge 0.0 0.0 Rainbow smelt 0.0 0.0 Round goby 0.0 0.0 Shorthead redhorse 0.0 0.0 Spottail shiner 0.5 0.0 Steelhead 0.0 0.0 Walleye 0.0 0.0 White sucker 0.0 0.0 Yellow perch 0.0 0.0 I Total 1.5 0.5 (continued) 20452 Cook 316b Baseline Final.doc 10/4/07 A-67 NormandeauAssociates, Inc.

3 16(b) PHASE I/ BASELINE FISH E & I STUDY Appendix Table 0. (Continued)

Diel Night (1800-Day (0600-1800) 0600)

CPUE CPUE Oct-05 Alewife 4.0 3.5 Bloater 16.0 1.5 Bluegill 0.5 0.0 Brook silverside 0.0 0.0 Chinook 0.0 0.0 Coho 0.0 0.0 Eastern banded killifish 0.5 0.0 Gizzard shad 13.5 47.0 Lake trout 0.0 0.0 Longnose sucker 0.0 0.0 Muskellunge 0.5 0.0 Rainbow smelt 0.0 0.0 Round goby 0.0 0.0 Shorthead redhorse 0.0 0.0 Spottail shiner 3.5 0.5 Steelhead 0.0 0.0 Walleye 0.0 0.0 White sucker 0.0 0.0 Yellow perch 0.5 0.0 Total 39.0 52.5 Nov-05 Alewife 0.0 0.5 Bloater 0.0 0.0 Bluegill 0.0 0.0 Brook silverside 0.0 0.0 Chinook 0.0 0.0 Coho 0.0 0.5 Eastern banded killifish 0.0 0.0 Gizzard shad 0.0 15.5 Lake trout 0.0 0.5 Longnose sucker 0.0 0.5 Muskellunge 0.0 0.0 Rainbow smelt 0.0 0.0 Round goby 0.0 0.0 Shorthead redhorse 0.0 0.0 Spottail shiner 3.0 1.5 Steelhead 0.0 0.0 Walleye 0.0 0.0 White sucker 0.0 0.0 Yellow perch 0.0 0.0 Total 3.0 19.0 20452 Cook 316b Baseline Final.doc 10/4/07 A-68 Normandeau Associates, Inc.

3 16(b) PHASE I/ BASELINE FISH E & I STUDY Appendix Table 0. (Continued)

Diel Night (1800-Day (0600-1800) 0600)

CPUE CPUE Apr-06 Alewife 0.0 0.0 Bloater 0.0 0.0 Bluegill 0.0 0.0 Brook silverside 0.0 1.5 Chinook 0.0 0.0 Coho 0.0 0.0 Eastern banded killifish 0.0 0.0 Gizzard shad 0.0 0.5 Lake trout 0.0 0.0 Longnose sucker 0.0 0.0 Muskellunge 0.0 0.0 Rainbow smelt 0.0 0.0 Round goby 0.0 7.0 Shorthead redhorse 0.0 0.0 Spottail shiner 0.0 5.0 Steelhead 0.0 0.0 Walleye 0.0 0.0 White sucker 0.0 1.0 Yellow perch 0.5 1.0 Total 0.5 16.0 May-06 Alewife 0.0 0.0 Bloater 0.0 .0.0 Bluegill 0.0 0.0 Brook silverside 0.5 0.0 Chinook 0.0 0.0 Coho 0.0 0.0 Eastern banded killifish 0.0 0.0 Gizzard shad 0.0 0.0 Lake trout 0.0 0.0 Longnose sucker 0.0 0.0 Muskellunge 0.0 0.0 Rainbow smelt 0.0 0.0 Round goby 0.5 2.0 Shorthead redhorse 0.0 0.0 Spottail shiner 2.5 29.5 Steelhead 0.0 0.0 Walleye 0.0 0.0 White sucker 0.0 0.0 Yellow perch 0.0 0.0 Total 3.5 31.5 20452 Cook 316b Baseline Final.doc 10/4/07 A-69 Normandeau Associates, Inc.

3 16(b) PHASE I/ BASELINE FISH E & I STUDY Appendix Table 0. (Continued)

Diel Night (1800-Day (0600-1800) 0600)

CPUE CPUE Jun-06 Alewife 0.5 4.5 Bloater 1.0 0.0 Bluegill 0.0 0.0 Brook silverside 0.0 0.0 Chinook 0.0 0.0 Coho 1.0 0.0 Eastern banded killifish 1.0 0.0 Gizzardshad 0.0 0.0 Lake trout 0.0 0.0 Longnose sucker 0.0 0.0 Muskellunge 0.0 0.0 Rainbow smelt 8.0 0.0 Round goby 0.0 0.0 Shorthead redhorse 0.0 0.0 Spottail shiner 61.0 28.0 Steelhead 0.0 0.0 Walleye 0.0 0.5 White sucker 0.0 0.0 Yellow perch 6.5 3.0 Total 79.0 36.0 Jul-06 Alewife 0.0 6.5 Bloater 0.0 0.0 Bluegill 0.0 0.0 Brook silverside 0.0 0.0 Chinook 0.5 0.0 Coho 0.0 0.0 Eastern banded killifish 0.0 0.0 Gizzard shad 0.0 0.0 Lake trout 0.0 0.0 Longnose sucker 0.0 0.0 Muskellunge 0.0 0.0 Rainbow smelt 0.0 0.0 Round goby 0.0 1.0 Shorthead redhorse 0.0 0.0 Spottail shiner 2.5 22.0 Steelhead 0.5 0.0 Walleye 0.0 0.0 White sucker 0.0 .0.5 Yellow perch 2.0 2.0 Total 5.5 32.0 20452 Cook 316b Baseline Final.doc 10/4/07 A-70 NormandeauAssociates, Inc.

3 16(b) PHASE II BASELINE FISH E & I STUDY Appendix Table 0. (Continued)

Diel Night (1800-Day (0600-1800) 0600)

CPUE CPUE Aug-06 Alewife 0.0 0.5 Bloater 0.0 0.0 Bluegill 0.0 0.0 Brook silverside 0.0 0.0 Chinook 0.0 .0.0 Coho 0.0 0.0 Eastern banded killifish 0.0 0.0 Gizzard shad 0.0 0.0 Lake trout .0.0 0.0 Longnose sucker 0.0 0.0 Muskellunge 0.0 0.0 Rainbow smelt 0.0 0.0 Round goby 0.0 0.5 Shorthead redhorse 0.0 0.0 Spottail shiner 0.0 0.5 Steelhead 0.0 0.0 Walleye 0.0 0.0 White sucker 0.0 0.0 Yellow perch 1.5 0.5 Total 1.5 2.0 Sep-06 Alewife 0.0 0.0 Bloater 0.0 0.0 Bluegill 0.0 0.0 Brook silverside 0.0 0.0 '

Chinook 0.0 0.0 Coho 0.0 0.5 Eastern banded killifish 0.0 0.0 Gizzard shad 0.0 0.0 Lake trout 0.0 0.0 Longnose sucker 0.0 0.0 Muskellunge 0.0 0.0 Rainbow smelt 0.0 0.0 Round goby 0.0 0.0 Shorthead redhorse 0.0 0.0 Spottail shiner 2.0 0.5 Steelhead 0.0 0.0 Walleye 0.0 0.0 White sucker 0.0 0.0 Yellow perch 0.0 1.5 Total 2.0 2.5 20452 Cook 316b Baseline Final.doc 10/4/07 A-71 Normandeau Associates, Inc.

3 16(b) PHASE!!I BASELINE FISH E & I STUDY Appendix Table 0. (Continued)

Diel Night (1800-Day (0600-1800) 0600)

CPUE CPUE Oct-06 Alewife 32.0 0.0 Bloater 40.5 0.0 Bluegill 0.0 0.0 Brook silverside 0.0 0.0 Chinook 0.0 0.0 Coho 0.0 0.0 Eastem banded killifish 0.0 0.0 Gizzard shad 0.0 0.5 Lake trout 0.0 0.5 Longnose sucker 0.0 0.0 Muskellunge 0.0 0.0 Rainbow smelt 0.5 0.0 Round goby 0.0 0.0 Shorthead redhorse 1.0 0.0 Spottail shiner 3.5 0.5 Steelhead 0.0 0.0 Walleye 0.0 0.0 White sucker 0.0 0.0 Yellow perch 0.0 0.0 Total 77.5 1.5 Nov-06 Alewife 0.0 0.0 Bloater 0.0 0.5 Bluegill 0.0 0.0 Brook silverside 0.0 0.0 Chinook 0.0 0.0 Coho 0.0 0.0 Eastern banded killifish 0.0 0.0 Gizzard shad 0.0 0.0 Lake trout 0.0 0.0 Longnose sucker 0.0 0.0 Muskellunge 0.0 0.0 Rainbow smelt 0.0 -0.0 Round goby 0.0 0.0 Shorthead redhorse 0.0 0.0 Spottail shiner 1.5 0.0 Steelhead 0.0 0.0 Walleye 0.0 0.0 White sucker 0.0 0.0 Yellow perch 0.0 0.5 Total 1.5 1.0 20452 Cook 316b Baseline Final.doc 10/4/07 A-72 NormandeauAssociates, Inc.

Appendix Table P. Water Quality Data Associated with Ichthyoplankton Sampling in the Vicinity of Cook Nuclear Plant, June CO)

N) 0 through November 2005 and April through November 2006.

0 0

Location=Inshore Station April May June July August September October November C,

2005 Surface Temperature (QC) 18.6 23.1 12.0 13.2 0,

Surface Dissolved Oxygen (mg/i) 9.8 9.1 9.9 10.8 2006 Surface Temfperature (° C) 7.3 10.5 19.4 23.0 23.2 22.2 9.9 CO Surface Dissolved Oxygen (mg/i) 13.3 12.4 10.7 8.5 8.9 9.2 10.9 4T C, Location=Intake Station (22 ft in depth) 2005 Surface Temperature (0 C) 26.0 27.2 19.5 22.8 12.8 12.9 Mid-depth Temperature (0 C) 22.4 24.7 17.3 22.6 11.9 12.8 I-Bottom Temperature (° C) 19.7 24.5 14.7 22.3 10.9 12.4 In Surface Dissolved Oxygen (mg/1) 8.9 7.8 9.4 9.1 9.5 10.5 Mid-depth Dissolved Oxygen (mg/i) 9.5 8.2 10.4 9.1 9.6 10.5 Bottom Dissolved Oxygen (mg/I) 10.3 8.2 11.2 9.2 9.5 10.4 2006 Surface Temperature (0 C) 6.4 10.5 18.2 23.9 22.4 22.1 10.6 Mid-depth Temperature (°.C) 6.4 9.6 17.5 22.4 21.8 20.8 9.7 Bottom Temperature (C) 6.4 9.0 17.1 19.0 21.2 20.8 9.5 Surface Dissolved Oxygen (mg/1) 13.3 12.4 10.9 8.7 9.0 9.2 10.7 Mid-depth Dissolved Oxygen (mg/1) 13.2 12.4 11.1 9.3 9.3 9.3 10.7 Bottom Dissolved Oxygen (mg/1) 13.3 12.5 11.3 10.8 9.4 9.2 10.9 Location=Experimental Station (40 ft in depth) 2005 Surface - 20 ft. Water Temperature (0 C) 23.5 25.4 17.0 22.6 12.6 12.8 0 30-40 ft Water Temperature (0 C) 12.0 24.5 11.7 20.2 10.5 12.2 Surface - 20 ft. Water Dissolved Oxygen (mg/I) 9.5 8.0 10.4 9.1 9.6 10.5 30-40 ft. Water Bottom Dissolved Oxygen (mg/I) 13.1 8.2 i 1.9 9.9 9.3 10.5 2006 Surface - 20 ft. Water Temperature (0 C) 6.1 9.4 17.4 23.0 21.4 21.3 10.0 a) 30-40 ft Water Temperature (0 C) .6.1 7.8 12.2 17.0 20.6 20.6 9.3 Surface - 20 ft. Water Dissolved Oxygen (mg/1) 13.4 12.4 11.3 9.3 9.2 9.2 10.7 30-40 ft. Water Bottom Dissolved Oxygen (mg/1) 13.3 12.6 13.5 10.9 9.4 9.2 11.1 Co 0

a) p

Appendix Table Q. Water Quality Data Associated with Gill Net (A), Otter Trawl (B), and Seine (C) Sampling in the Vicinity of Cook Nuclear Plant, June through November 2005 and April through November 2006.

0 A. Gill Net Location [ April May June July August September October November w Ca, Intake 2005 Surface Temperature (QC) 22 27.2 19.9 25.2 13.4 13.1

":3 Bottom Temperature (QC) 21.2 24.4 15.8 22.5 11.2 12.4 0* Surface Dissolved Oxygen (mg/i) 9.3 7.9 9.5 8.6 9.6 10.5  ::z Bottom Dissolved Oxygen (mg/1) 9.6 8.2 10.9 9 9.6 10.5 D3 C, 2006 Surface Temperature (o C) 6.4 10 18.3 24.2 22.9 22.4 10.8 Bottom Temperature (QC) 6.3 8.5 17.1 20.2 21.6 20.5 9.6 Surface Dissolved Oxygen (mg/1) 13.4 12.7 10.9 8.8 9 8.5 10.5 Bottom Dissolved Oxygen (mg/i) 13.3 12.7 11.4 10.1 9.2 8.6 10.6 rn 40 ft 2005 Surface Temperature (0 C) 22.5 27.8 19.6 24.6 13 13.2 C "A

Bottom Temperature (QC) 10.1 24.5 11.7 14.1 10.5 12.3 -n Z

Surface Dissolved Oxygen (mg/1) 9.3 7.6 9.7 8.7 9.7 10.5 Bottom Dissolved Oxygen (mg/1) 13.6 8.2 12.1 11.7. 9.3 10.4 2006 Surface Temperature (QC) 6.1 10.6 18.2. 24 22.4 22.5 11.1 Bottom Temperature (C) 6 7.8 11.7 15.1 21.1 20.4 9.4 Surface Dissolved Oxygen (mg/i) 13.6 12.4 11 8.9 9.1 8.5 10.7 Bottom Dissolved Oxygen (mg/1) 13.3 12.6 13.7 11.4 9.3 8.4 10.1 o

19

4) Appendix Table Q. (Continued)

CD 0

B. Otter Trawl a)

April May June July August September October November b.

03 0 5 ft 2005 Surface Temperature (QC) 12.3 13.2 Surface Dissolved Oxygen (mg/i) 9.9 11 C,,

hi 2006 Surface Temperature (0 C) 7.1 10.4 19.8 23 22.5 20.9 9.5 d,a Surface Dissolved Oxygen (mg/l) 13.2 12.4 10.7 8.9 8.9 8.7 10.9 Intake (22 ft) rn 2005 Surface Temperature (0 C) 22.4 27.1 20.3 25.5 12.4 12.7 rn Bottom Temperature (0 C) 21.2 24.4 15 22.5 12.3 Surface Dissolved Oxygen (mg/1) 9.3 7.8 9.6 8.7 9.8 10.8 Irn Bottom Dissolved Oxygen (mg/l) 9.7 8.5 11.3 9.1 10.7

---1 2006 Surface Temperature (0 C) 6.4 10 21.3 23.9 22.1 21 10 Bottom Temperature (0 C) 6.4 8.9 18.5 19 21.7 20.5 9.2 Surface Dissolved Oxygen (mg/1) 13.3 12.5 10.9 8.7 9 8.6 10.7 Bottom Dissolved Oxygen (mg/l) 13.2 12.5 11.1 10.8 9.2 8.7 11.1 Experimental (40 ft) 2005 Surface Temperature (0 C) 22 27.7 19.7 24.4 13.6 12.9 0

Bottom Temperature (0 C) 10.2 24.4 12 17.6. 12.2 Surface Dissolved Oxygen (mg/1) 9.3 7.6 9.9 8.6 9.8 10.7

0. Bottom Dissolved Oxygen (mg/i) 13.5 8 12.1 10.7 10.5 2006 Surface Temperature (C) 6.1 10.1 20.4 24 21.9 21.4 10.3 Bottom Temperature (0 C) 6.1 7.9 15.9 15.4 20.6 20.5 9.2 0

Surface Dissolved Oxygen (mg/1) 13.6 12.6 10.9 8.9 9.1 8.6 10.8 Bottom Dissolved Oxygen (mg/1) 13.2 12.6 12.1 11.9 9.5 8.6 10 C. Seine April May June July August September October November 2005 Surface Temperature(0 C) 18.7 25 12.8 Surface Dissolved Oxygen (mg/1) 9.7 8.8 10.7 2006 Surface Temperature (0 C) 8.5 10.6 19.5 23.1 23.1 21.5 9.9 Surface Dissolved Oxygen (mg/I) 12.5 12.3 10.8 8.4 9 8.7 10.5