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==5.0 REFERENCES==
==5.0 REFERENCES==


12 Table :No.3-1 ,LAST OF TABLES Caption Water Quality. Paraeter and Mean Zebra Mussel Densities Collected Monthly During the 12012 Sampling Effort. at LaSalle Intake.Water Quality Parameters and Mean Zebra Mussel. Densities Collected Monthly During the 2012 Sampling Effort at LaSalle Cooling Lake.Page No.3-2 6 7 ii LIST OF FIGURES.Figure No. Caption Page No.3-1 Temperature Profile at the LaSalle Station Intake for the Period of 5 April to 7 November, 2012., 3-2 Temperature Profile at LaSalle Lake for the Period of 5 .April'to 7 November, 2012. 10 iii  
12 Table :No.3-1 ,LAST OF TABLES Caption Water Quality. Paraeter and Mean Zebra Mussel Densities Collected Monthly During the 12012 Sampling Effort. at LaSalle Intake.Water Quality Parameters and Mean Zebra Mussel. Densities Collected Monthly During the 2012 Sampling Effort at LaSalle Cooling Lake.Page No.3-2 6 7 ii LIST OF FIGURES.Figure No. Caption Page No.3-1 Temperature Profile at the LaSalle Station Intake for the Period of 5 April to 7 November, 2012., 3-2 Temperature Profile at LaSalle Lake for the Period of 5 .April'to 7 November, 2012. 10 iii 1.0 INTRODUCTWON Exelon Nuclear has sponsored a zebra mussel (Dreissena.
 
==1.0 INTRODUCTWON==
Exelon Nuclear has sponsored a zebra mussel (Dreissena.
polymorpha) monitoring program since 1990 (ZEI 2003, LMS 2004-2005, HTDRILMS 2006-2007, I-DR Engineering, Inc. 2008-2011).
polymorpha) monitoring program since 1990 (ZEI 2003, LMS 2004-2005, HTDRILMS 2006-2007, I-DR Engineering, Inc. 2008-2011).
Stations electing to :continue monitoring in 2012 include Braidwood Station, Byron Station, and: LaSalle Station. Since the monitoring program began in 1990, zebra mussel colonization has, occurred at all three of these stations, to varying degrees.The principal objectives of these monitoring efforts at LaSalie are to:*Detect th~e presence and densities of zebra mussels at the intake structures and source water bodies.* Evaluate year-to-year changes in the zebra mussel populations, including settlement.
Stations electing to :continue monitoring in 2012 include Braidwood Station, Byron Station, and: LaSalle Station. Since the monitoring program began in 1990, zebra mussel colonization has, occurred at all three of these stations, to varying degrees.The principal objectives of these monitoring efforts at LaSalie are to:*Detect th~e presence and densities of zebra mussels at the intake structures and source water bodies.* Evaluate year-to-year changes in the zebra mussel populations, including settlement.
densities.
densities.
SProvide, a basis for examining possible effects of zebra mussels on Station operations..
SProvide, a basis for examining possible effects of zebra mussels on Station operations..
1 HDR Eugineering, The.  
1 HDR Eugineering, The.
 
2.0 METHODS 2.1 SUBSTRATE SAMPLING To determine zebra mussel settlement densities, artificial substrates were placed in front of the LaSalle Station river intake in the Illinois River. Three arrays of artificial substrate samplers were placed, which included two Type A substrates and one Type B substrate.
===2.0 METHODS===
2.1 SUBSTRATE SAMPLING To determine zebra mussel settlement densities, artificial substrates were placed in front of the LaSalle Station river intake in the Illinois River. Three arrays of artificial substrate samplers were placed, which included two Type A substrates and one Type B substrate.
Type A substrates consisted of two pieces of PVC pipe that were 15 cm (6 in.) long and had an inside diameter of 5.08 cm (2 in.) and an outside diameter of 6.08 cm (2.39 in.). Each pipe was cut in half lengthwise and .rejoined using hose. clamps. The PVC substrates were deployed by cable from'the railing above the intake in a central location.
Type A substrates consisted of two pieces of PVC pipe that were 15 cm (6 in.) long and had an inside diameter of 5.08 cm (2 in.) and an outside diameter of 6.08 cm (2.39 in.). Each pipe was cut in half lengthwise and .rejoined using hose. clamps. The PVC substrates were deployed by cable from'the railing above the intake in a central location.
Concrete blocks were used to anchor each sampling array to the bottom of the intake. One cumulative substrate sampler and one monthly (periodic) array was deployed at each sampling location.
Concrete blocks were used to anchor each sampling array to the bottom of the intake. One cumulative substrate sampler and one monthly (periodic) array was deployed at each sampling location.
Line 50: Line 45:
retrieval of Type A and Type B substrates was conducted as described above. Sampling was concluded on 7 November with the removall of all the substrates from the LaSalle Station intake and cooling lake. Monitoring was designed to provide information on accumulated infestation and growth of settled zebra mussels throughout the growing season.Settlement was determined by scraping the inside: and outside of both halves of each PVC pipe sampler. The area of the substrate that Was sampled was calculated by first finding the inside and outside circwmferences of the PVC pipe.c =,rd Where: c =circumference of a circle n --3.4 d --diameter of a circle The total area of PVC pipe that 'was ,sampled was calculated by summing thle total area sampled inside of the PVC pipe plus the total area sampled outside of the PVC pipe. The inside and outside area of the PVC pipe was calculated using the following equation:" A= cxl Where: A --area sampled ,e=circumference ofra circle 1 = length of the PVC pipe The number of mussels. found was then converted from number per square inch to the conventional number per square meter. Shell lengths (measured along the longest axis) were also measured for up to 50 individuals to obtain maximum, minimum, and mean sizes for each substrate.
retrieval of Type A and Type B substrates was conducted as described above. Sampling was concluded on 7 November with the removall of all the substrates from the LaSalle Station intake and cooling lake. Monitoring was designed to provide information on accumulated infestation and growth of settled zebra mussels throughout the growing season.Settlement was determined by scraping the inside: and outside of both halves of each PVC pipe sampler. The area of the substrate that Was sampled was calculated by first finding the inside and outside circwmferences of the PVC pipe.c =,rd Where: c =circumference of a circle n --3.4 d --diameter of a circle The total area of PVC pipe that 'was ,sampled was calculated by summing thle total area sampled inside of the PVC pipe plus the total area sampled outside of the PVC pipe. The inside and outside area of the PVC pipe was calculated using the following equation:" A= cxl Where: A --area sampled ,e=circumference ofra circle 1 = length of the PVC pipe The number of mussels. found was then converted from number per square inch to the conventional number per square meter. Shell lengths (measured along the longest axis) were also measured for up to 50 individuals to obtain maximum, minimum, and mean sizes for each substrate.
Shells less than 1.0 mm in length were measured to the nearest 25 microns using an ocular micrometer that was calibrated to a stage micrometer.
Shells less than 1.0 mm in length were measured to the nearest 25 microns using an ocular micrometer that was calibrated to a stage micrometer.
Shells greater than 1.0 mm in length were measured to the nearest half-millimeter using a standard metric ruler.3 3 IIDR Engineering, liuc.  
Shells greater than 1.0 mm in length were measured to the nearest half-millimeter using a standard metric ruler.3 3 IIDR Engineering, liuc.
 
2.3 WATER QUALITY MEASUREMENTS Four physicoehemical parameters (temperature, dissolved oxygen, p11 and conductivity) were measured in conjunction with the sampling program. These data were collected at each location prior to each sampling effort. All physicochemical measurements were made at one meter below the surface of the water. Temperature (0 C), dissolved oxygen (ppm), and conductivity (pxmhos)were measured using an ysI Model 85 Oxygen, Conductivity, Temperature, and Salinity meter.An Oakton WP pH Testerl was used to determine pH.Temperature monitors (Onset OptieStowAway Temp Loggers) were attached at mid-depth to the cumulative and monthly substrates to record hourly changes in temperature throughout the entire sampling period. Periodic temperature data. was downloaded on a monthly basis, while cumulative temperature recordings were monitored by a separate logger from 5 April through 7 November.4 4 ~ HDR Engineering, Inc.  
===2.3 WATER===
*3.0 RESULTS AND DISCUSSIION 3.1 ARTIFICIAL SUBSTRATE 3.1 .1 Intake Substrates One zebra mussel was collected on the monthly substr'atesfrom thle LaSalle Station river intake in July. The juvenile mussel measured 1.6 mm and represents
QUALITY MEASUREMENTS Four physicoehemical parameters (temperature, dissolved oxygen, p11 and conductivity) were measured in conjunction with the sampling program. These data were collected at each location prior to each sampling effort. All physicochemical measurements were made at one meter below the surface of the water. Temperature (0 C), dissolved oxygen (ppm), and conductivity (pxmhos)were measured using an ysI Model 85 Oxygen, Conductivity, Temperature, and Salinity meter.An Oakton WP pH Testerl was used to determine pH.Temperature monitors (Onset OptieStowAway Temp Loggers) were attached at mid-depth to the cumulative and monthly substrates to record hourly changes in temperature throughout the entire sampling period. Periodic temperature data. was downloaded on a monthly basis, while cumulative temperature recordings were monitored by a separate logger from 5 April through 7 November.4 4 ~ HDR Engineering, Inc.  
*3.0 RESULTS AND DISCUSSIION
 
===3.1 ARTIFICIAL===
 
SUBSTRATE 3.1 .1 Intake Substrates One zebra mussel was collected on the monthly substr'atesfrom thle LaSalle Station river intake in July. The juvenile mussel measured 1.6 mm and represents
: a. settlement density of 1 8/rn 2 (Table 3-1 ). Analysis of the surface cumulative substrate (0.5 mn)revealed heavy filamentous algae and bryoz~ans colonies covering the outside. of the substrate.
: a. settlement density of 1 8/rn 2 (Table 3-1 ). Analysis of the surface cumulative substrate (0.5 mn)revealed heavy filamentous algae and bryoz~ans colonies covering the outside. of the substrate.
The inside of the pipe contained numerous caddisfly larvae and byrozoan colonies.
The inside of the pipe contained numerous caddisfly larvae and byrozoan colonies.
The bottom cumulative substrate (1.5 m) was also covered both inside and outside with caddisfly larvae, bryozoans, and other aquatic'invertebrates.
The bottom cumulative substrate (1.5 m) was also covered both inside and outside with caddisfly larvae, bryozoans, and other aquatic'invertebrates.
 
3.1.2. COoling Lake Substrates No zebra mussels were collected on the monthly substrates from the LaSalle Station Cooling Lake throughout the seven month sampling period (Table 3-2). Cumulative substrates were 90-100% covered with bryozoan colonies.
====3.1.2. COoling====
Lake Substrates No zebra mussels were collected on the monthly substrates from the LaSalle Station Cooling Lake throughout the seven month sampling period (Table 3-2). Cumulative substrates were 90-100% covered with bryozoan colonies.
All depths sampled (1.0, 3.0, 5.0 :m) were observed to have a similar degree of colonization.
All depths sampled (1.0, 3.0, 5.0 :m) were observed to have a similar degree of colonization.
 
3.2 WATER QUALITY IMEASUREMNTS Physicochemical parameters (temperature, dissolved oxygen, pH and conductivity) were measured in conjunction with the sampling program are presented in Tables 3-1 and 3-2. Water quality measurements were taken during each of the sampling dates at the LaSalle Station intake and cooling water lake.3.2.1 Intake Water Quality Temperatures at the LaSalle Station intake ranged from 7.90 C on 7 November to 34.10 C on 7 July (Figure 3-1). Dissolved oxygen (DO) concentrations ranged from 6.30 ppm on 40Oetober to 9.8 ppm on 7 November (Table 3-1). Recordings for p1- ranged from 7.6 to 8.1 and conductivity ranged from 393 to 837 Mtrhos throughout the sampling season.5 5 E[I)DR Engineering, Inc.
===3.2 WATER===
QUALITY IMEASUREMNTS Physicochemical parameters (temperature, dissolved oxygen, pH and conductivity) were measured in conjunction with the sampling program are presented in Tables 3-1 and 3-2. Water quality measurements were taken during each of the sampling dates at the LaSalle Station intake and cooling water lake.3.2.1 Intake Water Quality Temperatures at the LaSalle Station intake ranged from 7.90 C on 7 November to 34.10 C on 7 July (Figure 3-1). Dissolved oxygen (DO) concentrations ranged from 6.30 ppm on 40Oetober to 9.8 ppm on 7 November (Table 3-1). Recordings for p1- ranged from 7.6 to 8.1 and conductivity ranged from 393 to 837 Mtrhos throughout the sampling season.5 5 E[I)DR Engineering, Inc.
Table 3-1. Water Quality Parameters and Mean Zebra Mussel Densities Collected Monthly During the 2012 Sampling Effort at LaSalle Intake.Water Quality Juveniles Adults Days/ Temp. DO Cond. Sample Date Sample Time (°C) .(ppm) pH (ptmhos) Depth Glass PVC Glass PVC 04/05/12 08:25 14.6 ,9.15 8.0 715- ---05/09112 34 08:00 17..8 7.80 7.6 393 -na na na na 05/30/12 21 08:00 :24.6 7.85 8.0 791 -, 07/05/12 36 08:05 31.3 7.90 7.8 763 1.5 -1.6mm-08/07/12 33 13:05 :30.6 7.06 7.7 760-- -09/10/12 34 13:40 25.9 8.40 :7.8 837 ---10/04/12.
Table 3-1. Water Quality Parameters and Mean Zebra Mussel Densities Collected Monthly During the 2012 Sampling Effort at LaSalle Intake.Water Quality Juveniles Adults Days/ Temp. DO Cond. Sample Date Sample Time (°C) .(ppm) pH (ptmhos) Depth Glass PVC Glass PVC 04/05/12 08:25 14.6 ,9.15 8.0 715- ---05/09112 34 08:00 17..8 7.80 7.6 393 -na na na na 05/30/12 21 08:00 :24.6 7.85 8.0 791 -, 07/05/12 36 08:05 31.3 7.90 7.8 763 1.5 -1.6mm-08/07/12 33 13:05 :30.6 7.06 7.7 760-- -09/10/12 34 13:40 25.9 8.40 :7.8 837 ---10/04/12.
22 08t.15 20.5 6.30 8.0 803-- --11/07/12 .34 09:10 10.2 9.80 8.1 637- ---
22 08t.15 20.5 6.30 8.0 803-- --11/07/12 .34 09:10 10.2 9.80 8.1 637- ---
Line 74: Line 59:
Depth Glass PVC Glass PVC 04/05/12 -09:15 18.8 9.20 8.4 1210-- --05/09/12 34 09:20 25.3 7.59 8.2 1368 ----05/30/12 21 08:54 27.0 7.16 8.5 1253 -----07/05/12 36i 09:05 34.8 7.35 8.4 1237 ---08/07/12 33 13:39 34.9. 8.62 8.4 1277 ----09/10/12 34 144.i5 30.3 9.53 8.5 1533 --- --10/04/12 22 08:50 25.5 6.28 8.7 1142.i11/07/12 34 09:50 17.6 9.20 8.8 1241- ---
Depth Glass PVC Glass PVC 04/05/12 -09:15 18.8 9.20 8.4 1210-- --05/09/12 34 09:20 25.3 7.59 8.2 1368 ----05/30/12 21 08:54 27.0 7.16 8.5 1253 -----07/05/12 36i 09:05 34.8 7.35 8.4 1237 ---08/07/12 33 13:39 34.9. 8.62 8.4 1277 ----09/10/12 34 144.i5 30.3 9.53 8.5 1533 --- --10/04/12 22 08:50 25.5 6.28 8.7 1142.i11/07/12 34 09:50 17.6 9.20 8.8 1241- ---
40:35'30 25 S 20 10 c "" ~ ,., *q" -Figure 3-1. Temperature Profile at the LaSalle Station Intake for the Period of 5 Aprilto 7 NoVemher,:
40:35'30 25 S 20 10 c "" ~ ,., *q" -Figure 3-1. Temperature Profile at the LaSalle Station Intake for the Period of 5 Aprilto 7 NoVemher,:
2012.  
2012.
 
3.2.2 Cooling Lake Water Quality Temperatures from the LaSalle Station cooling lake ranged from 1 6.26 C on i1 November to 37.20 C on 7 July (Figure 3-2). Dissolved oxygen (DO) concentrations ranged from 6.28 ppm on 4 October to 9.53 ppm on 10 September (Table 3-2). Recordings for pH ranged from 8.2 to 8.8 and conductivity ranged from 11~42 to 1533 l.unhos throughout the sampling season.9 HDR Engineering, Inc.  
====3.2.2 Cooling====
-40 35 30 e, 20 2 0 10 S 0 Figure. 3-2. Temperature Profile at LaSalie Lake for the Period of 5 April to 7 November, 2012.
Lake Water Quality Temperatures from the LaSalle Station cooling lake ranged from 1 6.26 C on i1 November to 37.20 C on 7 July (Figure 3-2). Dissolved oxygen (DO) concentrations ranged from 6.28 ppm on 4 October to 9.53 ppm on 10 September (Table 3-2). Recordings for pH ranged from 8.2 to 8.8 and conductivity ranged from 11~42 to 1533 l.unhos throughout the sampling season.9 HDR Engineering, Inc.  
4.0 SUMAR AND RECOMMENDATIONS In 2012, one zebra mussel Was found on the periodic (monthly) substrates from the river intake at.LaSalle. Station. This juvenile measured 1.5 mm in length and represents a settlement density of 18/rn 2.No zebra mussels were.detected on the r~iver intake cumulative substrates or ,any of the LaSalle Lake substrates. ,The cumulative substrates from both the river intake and LaSalle Lake were 90-100% covered by bryozoan colonization at all sample depths.Early detection of zebra mussel colonization is essential for uninterrupted Station operation.
-40 35 30 e, 20 2 0 10 S 0 Figure. 3-2. Temperature Profile at LaSalie Lake for the Period of 5 April to 7 November, 2012.  
 
===4.0 SUMAR===
AND RECOMMENDATIONS In 2012, one zebra mussel Was found on the periodic (monthly) substrates from the river intake at.LaSalle. Station. This juvenile measured 1.5 mm in length and represents a settlement density of 18/rn 2.No zebra mussels were.detected on the r~iver intake cumulative substrates or ,any of the LaSalle Lake substrates. ,The cumulative substrates from both the river intake and LaSalle Lake were 90-100% covered by bryozoan colonization at all sample depths.Early detection of zebra mussel colonization is essential for uninterrupted Station operation.
Because zebra mussels are present in the Illinois River, they are continually reintroduced to the cooling lake through make-up water. Continued monitoring is important to recognize the'circumstances associated with zebra mussel infestation and colonization.
Because zebra mussels are present in the Illinois River, they are continually reintroduced to the cooling lake through make-up water. Continued monitoring is important to recognize the'circumstances associated with zebra mussel infestation and colonization.
11 11 -HDR Engineering, Inc.  
11 11 -HDR Engineering, Inc.  

Revision as of 09:20, 7 May 2019

Hdr Engineering. 2013. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2012
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ER REFERENCES TO BE DOCKETED ATTACHMENT 10 fl ZEBA MUSSEL MONITO G PROG]A At LaSalle Nuclear Station, 2012 Prepared for EXELON .NUCLEAR Warrenville, 'illinois.

H=DR Engineering, Inc.Environmental Science & Engineering Consultants 10207 Lucas Road, Woodstock, Illinois 6009.8 TABLE OF CONTENTS Page No.TABLE OF CONTENTSi LIST OF TABLES ii LIST OF FIGURS iii

1.0 INTRODUCTION

I 2.0 METHODS 2 2.11 Artificial Substrate Samplinig 12 2.2 'Water Quality Measurements 4: 3.0 RESULTS AND DISCUSSION 5 3.1 Artificial Substrate Sampling .5 3.1.1 Intake Substrates

.5 3.1,2 Cooling Lake Substrates

.5 3:.2 Water Quality Measurements 5 3.2.1 Intake Water Quality 5 3.2.2: Cooling Lake Water Quality 9.4.0

SUMMARY

AND REC2OMMENDATIONS 11,

5.0 REFERENCES

12 Table :No.3-1 ,LAST OF TABLES Caption Water Quality. Paraeter and Mean Zebra Mussel Densities Collected Monthly During the 12012 Sampling Effort. at LaSalle Intake.Water Quality Parameters and Mean Zebra Mussel. Densities Collected Monthly During the 2012 Sampling Effort at LaSalle Cooling Lake.Page No.3-2 6 7 ii LIST OF FIGURES.Figure No. Caption Page No.3-1 Temperature Profile at the LaSalle Station Intake for the Period of 5 April to 7 November, 2012., 3-2 Temperature Profile at LaSalle Lake for the Period of 5 .April'to 7 November, 2012. 10 iii 1.0 INTRODUCTWON Exelon Nuclear has sponsored a zebra mussel (Dreissena.

polymorpha) monitoring program since 1990 (ZEI 2003, LMS 2004-2005, HTDRILMS 2006-2007, I-DR Engineering, Inc. 2008-2011).

Stations electing to :continue monitoring in 2012 include Braidwood Station, Byron Station, and: LaSalle Station. Since the monitoring program began in 1990, zebra mussel colonization has, occurred at all three of these stations, to varying degrees.The principal objectives of these monitoring efforts at LaSalie are to:*Detect th~e presence and densities of zebra mussels at the intake structures and source water bodies.* Evaluate year-to-year changes in the zebra mussel populations, including settlement.

densities.

SProvide, a basis for examining possible effects of zebra mussels on Station operations..

1 HDR Eugineering, The.

2.0 METHODS 2.1 SUBSTRATE SAMPLING To determine zebra mussel settlement densities, artificial substrates were placed in front of the LaSalle Station river intake in the Illinois River. Three arrays of artificial substrate samplers were placed, which included two Type A substrates and one Type B substrate.

Type A substrates consisted of two pieces of PVC pipe that were 15 cm (6 in.) long and had an inside diameter of 5.08 cm (2 in.) and an outside diameter of 6.08 cm (2.39 in.). Each pipe was cut in half lengthwise and .rejoined using hose. clamps. The PVC substrates were deployed by cable from'the railing above the intake in a central location.

Concrete blocks were used to anchor each sampling array to the bottom of the intake. One cumulative substrate sampler and one monthly (periodic) array was deployed at each sampling location.

The PVC substrates were attached to the cable approximately 0.5 and 1.5 meters below the surface of the water, respectively.

Artificial substrates were installed in front of the intake on 5 April. Retrieval of Type A substrates occurred on a: monthly basis and consisted of gently removing the series of PVC pipes from the. water and placing each individual sample into a labeled container containing a solution of 40% isopropyl alcohol. New PVC substrates were then placed on. the monthly array and returned to their original positions in the. water column. Cumulative substrates remained in the water from 5 April to 7 November.Type B substrate arrays consist of a microscope slide rack (Dura-Sampler) attached in the same manner as the PVC Type A arrays. Retrieval of Type B substrates occurred on a monthly basis and required gently removing the microscope slide samplers from the water and placing eight glass slides from each sampler into an appropriately labeled container containing 40% isopropyl alcohol. New labeled glass slides were then installed into the slide samplers and returned to their'original location.

PVC .and glass were chosen as principle substrates because it has been shown that PVC is a preferred substrate (Walz 1975), while glass slides allow for a standard measurement of settlement densities and examination under a low powered binocular microscope (Marsden 1992).Two Type A and one Type B sample arrays were placed within thae LaSalle Station Cooling Lake on 5 April. Substrates were set at depths of 1.0, .3.0 and 5.0 meters on a cable suspended between a surface float and a concrete anchor. Both cumulative and monthly substrates were set. Monthly 2 2 HDR Engineering, Inc.

retrieval of Type A and Type B substrates was conducted as described above. Sampling was concluded on 7 November with the removall of all the substrates from the LaSalle Station intake and cooling lake. Monitoring was designed to provide information on accumulated infestation and growth of settled zebra mussels throughout the growing season.Settlement was determined by scraping the inside: and outside of both halves of each PVC pipe sampler. The area of the substrate that Was sampled was calculated by first finding the inside and outside circwmferences of the PVC pipe.c =,rd Where: c =circumference of a circle n --3.4 d --diameter of a circle The total area of PVC pipe that 'was ,sampled was calculated by summing thle total area sampled inside of the PVC pipe plus the total area sampled outside of the PVC pipe. The inside and outside area of the PVC pipe was calculated using the following equation:" A= cxl Where: A --area sampled ,e=circumference ofra circle 1 = length of the PVC pipe The number of mussels. found was then converted from number per square inch to the conventional number per square meter. Shell lengths (measured along the longest axis) were also measured for up to 50 individuals to obtain maximum, minimum, and mean sizes for each substrate.

Shells less than 1.0 mm in length were measured to the nearest 25 microns using an ocular micrometer that was calibrated to a stage micrometer.

Shells greater than 1.0 mm in length were measured to the nearest half-millimeter using a standard metric ruler.3 3 IIDR Engineering, liuc.

2.3 WATER QUALITY MEASUREMENTS Four physicoehemical parameters (temperature, dissolved oxygen, p11 and conductivity) were measured in conjunction with the sampling program. These data were collected at each location prior to each sampling effort. All physicochemical measurements were made at one meter below the surface of the water. Temperature (0 C), dissolved oxygen (ppm), and conductivity (pxmhos)were measured using an ysI Model 85 Oxygen, Conductivity, Temperature, and Salinity meter.An Oakton WP pH Testerl was used to determine pH.Temperature monitors (Onset OptieStowAway Temp Loggers) were attached at mid-depth to the cumulative and monthly substrates to record hourly changes in temperature throughout the entire sampling period. Periodic temperature data. was downloaded on a monthly basis, while cumulative temperature recordings were monitored by a separate logger from 5 April through 7 November.4 4 ~ HDR Engineering, Inc.

  • 3.0 RESULTS AND DISCUSSIION 3.1 ARTIFICIAL SUBSTRATE 3.1 .1 Intake Substrates One zebra mussel was collected on the monthly substr'atesfrom thle LaSalle Station river intake in July. The juvenile mussel measured 1.6 mm and represents
a. settlement density of 1 8/rn 2 (Table 3-1 ). Analysis of the surface cumulative substrate (0.5 mn)revealed heavy filamentous algae and bryoz~ans colonies covering the outside. of the substrate.

The inside of the pipe contained numerous caddisfly larvae and byrozoan colonies.

The bottom cumulative substrate (1.5 m) was also covered both inside and outside with caddisfly larvae, bryozoans, and other aquatic'invertebrates.

3.1.2. COoling Lake Substrates No zebra mussels were collected on the monthly substrates from the LaSalle Station Cooling Lake throughout the seven month sampling period (Table 3-2). Cumulative substrates were 90-100% covered with bryozoan colonies.

All depths sampled (1.0, 3.0, 5.0 :m) were observed to have a similar degree of colonization.

3.2 WATER QUALITY IMEASUREMNTS Physicochemical parameters (temperature, dissolved oxygen, pH and conductivity) were measured in conjunction with the sampling program are presented in Tables 3-1 and 3-2. Water quality measurements were taken during each of the sampling dates at the LaSalle Station intake and cooling water lake.3.2.1 Intake Water Quality Temperatures at the LaSalle Station intake ranged from 7.90 C on 7 November to 34.10 C on 7 July (Figure 3-1). Dissolved oxygen (DO) concentrations ranged from 6.30 ppm on 40Oetober to 9.8 ppm on 7 November (Table 3-1). Recordings for p1- ranged from 7.6 to 8.1 and conductivity ranged from 393 to 837 Mtrhos throughout the sampling season.5 5 E[I)DR Engineering, Inc.

Table 3-1. Water Quality Parameters and Mean Zebra Mussel Densities Collected Monthly During the 2012 Sampling Effort at LaSalle Intake.Water Quality Juveniles Adults Days/ Temp. DO Cond. Sample Date Sample Time (°C) .(ppm) pH (ptmhos) Depth Glass PVC Glass PVC 04/05/12 08:25 14.6 ,9.15 8.0 715- ---05/09112 34 08:00 17..8 7.80 7.6 393 -na na na na 05/30/12 21 08:00 :24.6 7.85 8.0 791 -, 07/05/12 36 08:05 31.3 7.90 7.8 763 1.5 -1.6mm-08/07/12 33 13:05 :30.6 7.06 7.7 760-- -09/10/12 34 13:40 25.9 8.40 :7.8 837 ---10/04/12.

22 08t.15 20.5 6.30 8.0 803-- --11/07/12 .34 09:10 10.2 9.80 8.1 637- ---

Table 3-2. Water Quality Parameters and Mean Zebra Mussel Densities Collected Monthly During the 2012 Sampling Effort at LaSalle Cooling Lake.Water Quality Juveniles Adults Days! Temp. DO Cond. Sample Date Sample Time (0 C) (ppm) PH .(jitmhos)

Depth Glass PVC Glass PVC 04/05/12 -09:15 18.8 9.20 8.4 1210-- --05/09/12 34 09:20 25.3 7.59 8.2 1368 ----05/30/12 21 08:54 27.0 7.16 8.5 1253 -----07/05/12 36i 09:05 34.8 7.35 8.4 1237 ---08/07/12 33 13:39 34.9. 8.62 8.4 1277 ----09/10/12 34 144.i5 30.3 9.53 8.5 1533 --- --10/04/12 22 08:50 25.5 6.28 8.7 1142.i11/07/12 34 09:50 17.6 9.20 8.8 1241- ---

40:35'30 25 S 20 10 c "" ~ ,., *q" -Figure 3-1. Temperature Profile at the LaSalle Station Intake for the Period of 5 Aprilto 7 NoVemher,:

2012.

3.2.2 Cooling Lake Water Quality Temperatures from the LaSalle Station cooling lake ranged from 1 6.26 C on i1 November to 37.20 C on 7 July (Figure 3-2). Dissolved oxygen (DO) concentrations ranged from 6.28 ppm on 4 October to 9.53 ppm on 10 September (Table 3-2). Recordings for pH ranged from 8.2 to 8.8 and conductivity ranged from 11~42 to 1533 l.unhos throughout the sampling season.9 HDR Engineering, Inc.

-40 35 30 e, 20 2 0 10 S 0 Figure. 3-2. Temperature Profile at LaSalie Lake for the Period of 5 April to 7 November, 2012.

4.0 SUMAR AND RECOMMENDATIONS In 2012, one zebra mussel Was found on the periodic (monthly) substrates from the river intake at.LaSalle. Station. This juvenile measured 1.5 mm in length and represents a settlement density of 18/rn 2.No zebra mussels were.detected on the r~iver intake cumulative substrates or ,any of the LaSalle Lake substrates. ,The cumulative substrates from both the river intake and LaSalle Lake were 90-100% covered by bryozoan colonization at all sample depths.Early detection of zebra mussel colonization is essential for uninterrupted Station operation.

Because zebra mussels are present in the Illinois River, they are continually reintroduced to the cooling lake through make-up water. Continued monitoring is important to recognize the'circumstances associated with zebra mussel infestation and colonization.

11 11 -HDR Engineering, Inc.

5.0 REFERENCES

HDR Engineering, Inc. 2012. Zebra Mussel Monitoring Program 201.1. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.HDR Engineering, Inc. ,2011I. Zebra Mussel Monitoring Program 2010. Prepared for Exelon Nuclear. Warrenvilie, IL. 1 2 pp.HDR Engineering, Inc. 2010. Zebra Mussel Monitoring Program 2009. Prepared for Exelon Nuclear. Warrenviile, IL. 12 pp.HDR Engineering, Inc. 2009. Zebra Mussel Monitoring Program 2008. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.at LaSalle Nuclear Station, at LaSalle Nuclear Station, at LaSalle Nuclear Station, at LaSalle Nuclear Station, H.DR Engineering, Inc. 2008. Zebra MUssel Monitoring Program at LaSalle Nuclear Station, 2007. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.HDRiLMS. 2007. Zebra Mussel Monitoring Programn at LaSaile Nuclear Station, 2006.Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.HDR/LMS. 2006. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2005.Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.Lawler, Matusky & Skelly Engineers, LLP (LMS). 2005. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2004. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.Lawler, Matusky & Skelly Engineers, LLP (LMS). 2004. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2003. Prepared for Exelon Nuclear.:

Warrenville, IL. 12 pp.Marsden, J, E. 1992. Standard Protocols for Monitoring and Sampling Zebra Mussel Illinois Natural: History Survey. Biological Notes 138, April 1992.Walz, V. N. 1975'. The Settlement of Larvae of Dreissena polymorpha on Artificial Substrates.

Arch. ilydrobiol.

Suppl. 47: 423-431.Zambrana Engineering, Inc. 2003. Byron Station 2002 Zebra Mussel (Dreissena polymorpha)

Monitoring Program Annual Report.12Engineering, Inc.

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