ML15273A419

EA Engineering, Science, and Technology, Inc. 2014. LaSalle County Station 2013 Fish and Benthos Monitoring and Historical Fish and Benthos Comparisons. Deerfield, Il. May 2014

ML15273A419
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Site:	LaSalle
Issue date:	05/28/2014
From:	Monzingo R, Sneen M EA Engineering, Science, & Technology
To:	Exelon Generation Co, Office of Nuclear Reactor Regulation
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ER REFERENCES TO BE DOCKETED ATTACHMENT 5

LASALLE COUNTY STATION 2013 FISH AND BENTHOS MONITORING AND HISTORICAL FISH AND BENTHOS COMPARISONS Preparedfor:

Exelon Generation Company, LLC 200 Exelon Way Kennett Square, Pennsylvania Preparedby:

BA Engineering, Science, and Technology, Inc.

444 Lake Cook Road, Suite 18 Deerfield, Illinois 60015 May 2014

LASALLE COUNTY STATION 2013 FISH AND BENTHOS MONITORING AND HISTORICAL FISH AND BENTHOS COMPARISONS Preparedfor:

Exelon Generation Company, LLC 200 Exelon Way Kennett Square, Pennsylvania Preparedby:

EA Engineering, Science, and Technology, Inc.

444 Lake Cook Road, Suite 18 Deerfield, Illinois 60015 Marty Sneen Date Project Manager

~hC~.AJ C ~aII~yu~o 5/28/2014 Dr. Richard Monzingo Date Project Director May 2014 15004.03 1040

TABLE OF CONTENTS

1. INTRODUCTION................................................. *............................... 1

2. METHODS ......................... ............................................................... 2 2.1 FISH ............. .......................................................................... 2 2.1.1 Field and Laboratory .............................................................. 2 2.1.2 Data Handling and Analysis .................................................... 3 2.2 Benthos.................................................................................... 4 2.2.1 Field and Laboratory............................................................. 4 2.2.2 Data Handling and Analysis..................................................... 5 2.3 PHYSICOCHEMICAL MEASUREMENTS .......... ............................... 6

3. RESULTS.......................................................................................... 7 3.1 FISH................................................................................. i...... 7 3.1.1 Electrofishing ........... .................................................... ".....7 3.1.2 Seining ................................. ............................................ 8 3.1.3 DELT Anomalies ................................................................... 9 3.1.4 Relative Weight .................................................................. 9 3.2 BENTHOS ................................................................................ 9 3.2.1 Hester-Dendy ....................... ............................................. 10 3.2.2 Ponar and Kick Net Samples ............ ......................................... 10

4. DISCUSSION ................. .................................................................. 12 4.1 FISH ..................................................................................... 12 4.1.1 Operational Studies............................................................. 12 4.1.2 Pre-Operational Studies........................................................ 13 4.1.3 Summary ......................................................................... 15 4.2 BENTHOS............................................................................... 17 4.2.1 Operational Studies ............................................................. 17 4.2.2 Pre-Operational Studies ................................ ....... 18 i

TABLE OF CONTENTS (CONTINUED) 4.2.3 Summ ary .....................................................

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5. REFERENCES................................................................................... 22 FIGURES TABLES APPENDIX A: LaSalle County Station Fish Data, August 2013 APPENDIX B: LaSalle County Station Benthic Macroinvertebrate Data, August 2013 ii

LIST OF FIGURES Number Title 1 2013 LaSalle County Station Fish and Benthos Monitoring iii

LIST OF TABLES Number Title 1 Intercept (a) and Slope (b) Parameters for Standard Weight (Ws) Equations with Minimum Total Lengths (mm) Recommended for Application 2 List of Common and Scientific Names of Fish Taxa Collected Near LaSalle Station, August 2013 3 Number, Biomass, and Relative Abundance of Fish Collected Near LaSalle Station, August 2013 4 Fish Captured at each Electrofishing and Seining Location near LaSalle Station, August 2013 5 Summary of DELT (Deformity, Erosion, Lesion, and Tumor) Anomalies on Fish Captured by Electro fishing and Seining near LaSalle Station, August 2013 6 Mean Relative Weights of Fish Collected near LaSalle Station, August 2013 7 Benthic Macroinvertebrate Taxa Collected from the Illinois River in the Vicinity of LaSalle County Station, August 2013 8 Density (#/in 2) and Relative Weight (%) for Benthic Macroinvertebrate Taxa Collected in Hester-Dendy Samples near LaS alle Station, August 2013 9 Density (#/m 2) and Relative Weight (%) for Benthic Macroinvertebrate Taxa Collected in Ponar Samples near LaSalle Station, August 2013 10 Density (#/m 2 ) and Relative Abundance (%) for Benthic Macroinvertebrate Taxa Collected in Ponar Samples from the Rock River near Byron Station, August 1999 11 Pre-operational HD Mean Density (#/m 2) and Total Taxa Richness for Locations 1 - 4 on the Illinois River, 1974 - 1978 12 Pre-operational Ponar and Dip Net Mean Density (#/m2) and Total Taxa Richness for Locations 1 - 4 on the Illinois River and Locations 5 and 6 on South Kickapoo Creek, 1974- 1978 iv

1. INTRODUCTION Fish, benthos, and physicochemical monitoring was conducted during summer 2013 in the Illinois River upstream and downstream of the LaS alle County Station to collect current aquatic biological information needed to support preparation of the Environmental Report as part of a U.S. Nuclear Regulatory Commission (NRC) license renewal application. The 2013 data were compared with historical data to determine if changes in the Illiiiois River have occurred that could be related to station operation.

LaSalle County Station is located near the Illinois River in LaSalle County about 75 miles southwest of Chicago and 11 miles southeast of Ottawa, Illinois. Makeup cooling water is withdrawn from the Illinois River at river mile 249.5, four miles north of LaSalle County Station and three miles upstream of the Marseilles Dam. LaSalle County Station has two generating units and a closed cycle cooling system that uses a perched cooling pond. Blowdown water from the cooling pond discharges into the Illinois River 275 meters downstream of the makeup water intake structure.

The 2013 monitoring program was comprised of two primary elements.

• Fish sampling using electrofishing and seining gear, and collection of associated physicochemical measurements at locations sampled during previous monitoring efforts.

• Benthos sampling using artificial substrate samplers and a grab sampler at locations that were established during pre-operational studies.

The objectives of the 2013 LaSalle program were to:

• Determine the current species composition, relative abundance, and distribution of fish within the study area and compare results with available historical data and pre-operational studies.

• Determine current taxa richness, density, and relative abundance of benthos within the study area and compare results with available historical data and pre-operational studies.

BA Engineering, Science, and Technology (BA) was contracted by the Exelon Generation Company, LLC to perform these tasks.

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2. METHODS 2.1 FISH Fish sampling was conducted at three locations in the Illinois River (Locations 1 and 2) and one location in the mouth of South Kickapoo Creek (Location 5) downstream of the blowdown discharge (Figure 1). The river locations represent Sampling areas upstream of the cooling lake blowdown discharge (Location 1) and downstream of the discharge point (Location 2). River and creek locations were the same as those sampled during pre-operational studies. Fish sampling was conducted during August 2013.

2.1.1 Field and Laboratory Fish were sampled by electrofishing and seining. Electro fishing was conducted using a boat-mounted boom-type electrofishing system utilizing a 230-V, 5,000 W, 3-phase AC generator equipped with volt/amp meters and a safety cut-off switch. Sampling began no earlier than one-half hour after sunrise and did not extend beyond one-half hour before sunset. Each location was sampled in a downstream direction for 500 meters and time elapsed was recorded. The crew consisted of a boat driver and one dipper; each equipped with long-handled, 3/16-inch mesh dip nets for retrieval of stunned fish. Shoreline seining was the second collection method used at each location. A straight seine 25 ft long by 6 ft deep with 3/16-inch ace mesh was used. Two seine hauls were made at each location in a downstream direction, each covering approximately 15 meters of shoreline. Seining and electrofishing were conducted on different days to avoid potential bias caused by each sampling effort.

All fish collected were identified to the lowest possible taxonomic level and counted. For each gear type, at each location, up to 25 randomly selected individuals of each species were measured for total length (millimeters) and weighed (grams). Any remaining individuals of a species were counted and batch weighed. Minnow species (Cyprinidae), excluding carp, goldfish, or carp/goldfish hybrids, were counted and batch weighed only. Specimens that could not be positively identified in the field were preserved in 10% formalin and returned to the laboratory for positive identification. In the laboratory, fish were processed in the same manner as in the field. Fish identifications were conducted utilizing current taxonomic keys or publications including Becker (1983), Pflieger (1975), Smith (1979), and Trautman (1981).

All fish collected were examined for external anomalies. External anomalies were classified as DELT anomalies (deformities, _erosions, lesions, and _tumors; Ohio EPA 1987, 1989), external parasites, or "other" abnormalities. The following is a review of DELT anomalies and their causes in freshwater fishes (Ohio EPA 1989):

1) Deformities - These anomalies can affect the head, spine, fins, and have a variety of causes including toxic chemicals, viruses, bacteria (e.g., Mycobacterium sp.), and protozoan parasites (e.g., Myxosoma cerebalis) (Ohio EPA 1989).

2) Erodedfins - These are the result of chronic disease principally caused by flexibacteria invading the fins causing a necrosis of the tissue (Post 1983). Necrosis of the fins may also be caused by gryodactylids, a small trematode parasite (Ohio EPA 1989).

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For this study, fin erosion was separated into three categories: slight erosion -<1/3 of fin eroded; moderate erosion -1/3 -2/3 of fin eroded, and severe erosion ->2/3 of fin eroded.

3) Lesions and Ulcers - These appear as open sores or exposed tissue and can be caused by viral (e.g., Lymphocystis sp.) or bacterial (e.g., Flexibactercolumnaris, Aeromonas spp., Vibrio sp.) infections (Ohio EPA 1989).

4) Tumors - Tumors result from the loss of carefully regulated cellular proliferative growth in tissue and are generally referred to as neoplasia. Tumors in wild fish populations can be the result of exposure to toxic chemicals. Baumann et al. (1987) identified polynuclear aromatic hydrocarbons (PAHs) as the cause of hepatic tumors in brown bullheads in the Black River (Ohio). Viral infections (e.g.,

Lymphocystis) can also cause tumors. Parasites (e.g., Glugea anomalaand Ceratomyxa shasta; Post 1983) may cause tumor-like masses, but are not considered tumors. Parasite masses can be squeezed and broken between the thumb and forefinger whereas true tumors are firm and not easily broken (Ohio EPA 1989).

Only those anomalies visible to the naked eye were recorded. The exact counts of anomalies present (e.g., the number of tumors or lesions per fish) were not recorded. An external anomaly is defined as the presence of externally visible skin or subcutaneous disorders, and is expressed as percent of affected fish among all fish processed (Ohio EPA 1989).

2.1.2 Data Handling and Analysis Field and laboratory data were entered on forms compatible for computer entry following serialization, diga-coding, and QA/QC checks. Data were managed in a SAS format (Release 8.2) to provide flexibility in reporting study results. Data developed from electrofishing and seining were reported as number and percent abundance for each species and kept separate by location, gear type, and sampling period. Electrofishing data were also reported as catch-per-effort (CPE, number/hr) by location and sampling period. Relative weight (Wr) was calculated for all applicable species. All observed occurrences of DELT anomalies were summarized in tabular form and reported as percent occurrence by species for each sampling period.

Fish condition was evaluated using the relative weight (Wr) index (Wege and Anderson 1978). This index represents a refinement of the relative condition factor concept and allows for interpopulational comparisons by making the standard weight-length regression species-specific rather than population-specific. Relative weight was calculated as:

Wr = W/Ws x 100 Where W is the measured weight, Ws is the standard weight and Wr is the length-specific standard weight predicted by a weight-length regression constructed to represent the species as a whole.

Length-specific standard weight functions are in the form:

log 10 Ws = a + (b x lOgl0 total length) where a (intercept) and b (slope) account for genetically determined shape characteristics of a 3

species and yield Wr values of 100 at particular times of the year for fish that have been well fed (Anderson and Gutreuter 1983).

Intercept, slope, and minimum length values for the Ws equation have been published for 36 speci~es and one hybrid (Bister et al. 2000; Anderson and Neumann 1996; Murphy et al. 1991) (Table 1).

Minimum lengths are established because the accuracy in weighing fish decreases markedly for smaller individuals and minimum lengths represent the length at which the variance to mean ratio for log10 sharply increases (Murphy et al. 1991).

Appendix A provides the LaSalle County Station fish data.

2.2 BENTHOS Benthos sampling was conducted at the four locations in the Illinois River (Locations 1, 2, 3, and

4) that were established during preoperational studies (Figure 1). Sampling was conducted upstream of the intake (Location 1), immediately downstream of the blowdown discharge (Location 2), downstream of the blowdown discharge and upstream of South Kickapoo Creek (Location 3), and downstream of South Kickapoo Creek (Location 4). Each location was sampled using Hester-Dendy (HD) artificial substrate samplers and a Ponar grab sampler.

Artificial substrates were set in June and retrieved in the August. Ponar samples were collected in August when the artificial substrates were recovered.

2.2.1 Field and Laboratory Each modified HD artificial substrate sampler consisted of eight 3x3-inch plates constructed from 1/8, inch tempered hardboard and twelve 1/8 plastic spacers. The plates and spacers were arranged on a 1/4 inch eyebolt so that each sampler had three 1/8 inch spaces, three 1/4 inch spaces, and one 3/8 inch space among the plates. The total surface area of a single sampler, excluding the eyebolt, was 0.093 m2 (1.01 22ft ). A single HD sample consisted of a five HD sampler array, arranged on a single cinder block, and deployed on the stream bottom. Duplicate HD sets were deployed at each location to minimize the potential loss of samplers (e.g., from vandalism). The HD samplers were set on 19 June 2013 and retrieved approximately eight weeks later on 12 August 2013. Retrieval of the HiDs was accomplished by enclosing the samplers in a fine-mesh container and then carefully lifting the sampler array and container to the surface. The HDs were placed into a single labeled container, and preserved with 10%

formalin.

The Ponar samples were collected in conjunction with the HD sample retrieval. Ponar samples from each location consisted of four composite grab samples at each location using a full-sized 523 cm 2 (81 in 2) Ponar dredge sampler. Each sample was sieved in the field using an U.S.

Standard No. 35 (500 pm) mesh sieve and preserved. The material obtained in each sample was examined to qualitatively determine substrate characteristics and percent composition.

Upon arrival at the laboratory, the samples were logged in and accounted for. Based on measured current velocity, the amount of silt/debris caught on the samplers and the numbers and types of organisms observed during retrieval, one of the duplicate HD arrays from each location was initially processed. The second HD array was kept as a backup. The five HDs from each 4

array were disassembled in a water filled enamel pan and cleaned of organisms and debris. This mixture was then passed through a No. 60 (250 jtm mesh) U.S. Standard Testing Sieve and preserved in labeled containers containing 10% formalin.

Sorting of each HD and Ponar grab sample was conducted in gridded petri dishes under a dissecting stereo-scope at 10OX magnification. The samples were initially pre-picked to remove any large or rare taxa (less than 20 individuals/sample) prior to subsampling. When necessary, a Motoda sample splitter was used to subsample until a manageable number of organisms was achieved. A minimum of 250 organisms was removed from the fractionated samples.

Organisms from all sample types were sorted to higher taxonomic levels (generally Class or Order level) and preserved separately in labeled vials containing 70% ethyl alcohol. Sorted samples were routinely checked by experienced EA personnel to assure a consistent level of quality and sorting efficiency.

Macroinvertebrate identifications were made to the 'lowest practical taxonomic level using the most current literature available. Chironomidae (midge) larvae were cleared in 10% potassium hydroxide and mounted in CMC-10 on glass slides prior to identification. For all sample types, specimens were enumerated, coded, and recorded on a standard laboratory bench sheet for data processing.

2.2.2 Data Handling and Analysis The following data were used for spatial and temporal comparisons:

• 1) Density (no./m 2 ) - Density is the total number of individuals collected within a square meter. Extremely high or low density relative to other locations may be indicative of environmental stress.

2) Relative Abundance (%) - Relative abundance is the percent each taxon composes within a sample. Dominance by one taxon or relatively few taxa may suggest greater environmental stress.

3) Dominant Taxa - Based on relative abundance, similarities in the top five dominant taxa among stations suggests balance within the benthic community. Numerical dominance by one or a few taxa may suggest impairment.

4) Total Taxa Richness - Total number Of different types of macroinvertebrates. Generally, the higher the taxa richness, the better the quality of the benthic community.

5) EPT Taxa Richness - Collectively, Ephemeroptera, Plecoptera, and Trichoptera are referred to as EPT. EPT richness is the number of taxa identified from these groups.

Since EPT taxa are generally considered to be intolerant of environmental stress, a

• relatively higher number of EPT taxa typically represent a quality benthic community.

6) Tolerance Values - DeveloPed by the Illinois Environmental Protection Agency (IEPA 1987; Tetra Tech 2007) tolerance values are used to generally characterize the environmental sensitivity of the benthic community. The tolerance values were derived 5

for individual macroinvertebrate taxa and reflect an increasing level of pollution tolerance from 0 (least tolerant) to 11 (most tolerant). A benthos community dominated by taxa with lower tolerance values is typically indicative of a healthy benthic community while a higher tolerance values suggest some degree of impairment.

Appendix B provides the LaS alle County Station macroinvertebrate data.

2.3 PHYSICOCHEMICAL MEASUREMENTS Water temperature, dissolved oxygen concentration, percent oxygen saturation, specific conductance, and water transparency using a Secchi disk were measured at each electrofishing location. All measurements, except Secchi depth, were made at mid-depth in the water column.

Instruments Used to measure temperature were checked against a calibrated thermometer.

Instruments used to measure DO were calibrated before each measurement. In addition, immediately before each sampling day, they were checked against the Winkler method as specified in Standard Methodsfor the Examination of Water and Wastewater (current edition).

The conductivity meter was also checked against a standard before each electro fishing sampling day. Percent oxygen saturation was determined from the relationship between dissolved oxygen in the water and water temperature.

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3. RESULTS 3.1 FISH In total, 23 species among seven families were collected by electro fishing and seining from the study area in 2013 (Table 2). Diversity was highest among the carp/minnow, sunfish, and sucker families, which were represented by 12, four, and three species, respectively.

The three locations sampled in August 2013 yielded a total of 1,295 fish weighing 21.2 kg (Table 3). Emerald Shiner (78 percent), Spotfin 'Shiner (10 percent), Gizzard Shad (4 percent), Bullhead Minnow (2 percent), and Sand Shiner (2 percent) were the five most abundant species collected, comprising nearly 94 percent of the total catch. Gizzard Shad (21 percent), Smallmouth Buffalo (20 percent), Freshwater Drum (18 percent), Common Carp (10 percent), Emerald Shiner (9 percent), Channel Catfish (7.5 percent), and Golden Redhorse (6 percent) dominated the catch in terms of biomass (Table 3). No endangered or threatened species were collected during this study (Illinois Endangered Species Protection Board 2011).

3.1.1 Electrofishing Electro fishing yielded 79 of the 1,295 total fish (61 percent) collected during the 2013 survey (Table 3). Fourteen species were collected, dominated by Gizzard Shad (48 percent), Spotfin Shiner (16.5 percent), Smalimouth Buffalo (6 percent), Golden Redhorse (5 percent),

Smallmouth Bass (4 percent), and Freshwater Drum (4 percent). All remaining species were represented by only one or two individuals.

Overall, catch rates (CPEs) were generally similar at the two electro fishing locations sampled in 2013 where 74 fish/hour were collected at Location 1 upstream of the LaSalle County Station discharge compared to 86 fish/hour collected at Location 2 downstream (Table 4). Gizzard Shad and Spotfin Shiner dominated the catch at both locations. However, Gizzard Shad were considerably more abundant downstream of the plant's discharge (28 fish) compared to upstream of it (10 fish),' whereas Spotfin Shiner were more abundant upstream (9 fish) compared to downstream (4 fish). All remaining species were collected in low numbers and were fairly evenly distributed between the two zones sampled (Table 4). However, species richness was moderately higher upstream of the plant's discharge (13) compared to downstream of it (9),

driven in large part by the reduced number of sunfish species collected downstream (1) compared to upstream (4). The increased number and diversity of sunfish collected upstream of the LaSalle County Station's discharge may be attributable to better habitat quality, particularly regarding instream cover. Eight species, Gizzard Shad, Emerald Shiner, Spotfin Shiner, Smallmouth Buffalo, Golden Redhorse, Channel Catfish, Largemouth Bass, and Freshwater Drum were collected at both sampling locations (Table 4).

Sport fish species collected during the 2013 electrofishing survey included Channel Catfish, Green Sunfish, Bluegill, Smallmouth Bass, Largemouth Bass, and Freshwater Drum.

• Collectively, these species comprised 16.5 percent of the total catch by number with individual species contributions ranging from 1 to 4 percent of the total catch (Table 4). Most of the 7

aforementioned sport fish species were represented by only one to two individuals, consisting primarily of Age I or II fish, with the exception of Channel Catfish. Two Channel Catfish measuring 365 and 500mm, likely representing Age III and Age V fish respectively, were collected (Appendix A). No young-of-year (YOY) sport fish were collected in 2013.

Water quality measurements collected at each location during the 2013 electro fishing survey are summarized below:

(C) (rag/L) (% Saturation) (ptS/cm) (cm)

Location Temperature Dissolved Oxygen Dissolved Oxygen Specific Conid. Secchi 1 27.7 10.2 128 767 68 2 28.1 8.5 108 945 64 Overall, water quality measurements varied only slightly throughout the study area. Water temperature and specific conductance values were only slightly higher downstream of the plant's discharge compared to upstream of it, whereas dissolved oxygen and percent saturation were marginally higher upstream. DO concentrations were consistently above the General Use minimum standards effective 16 May 2013 of 3.5 ppm at any time August through February (Illinois General Assembly Administrative Code 35). Water clarity was comparable upstream and downstream of the plant and was adequate for the field crew to observe and retrieve fish stunned by the electrofishing gear. All of the physicochemical measurements were within the range of values suitable to the native fish fauna of the Illinois River.

3.1.2 Seining Fourteen species were represented among the 1,216 fish collected seining (Table 4). Emerald Shiner (83 percent), Spotfin Shiner (9.5 percent), Bluntuose Minnow (2 percent), and Sand Shiner (2 percent) dominated the catch. These four species comprised nearly 97 percent of all fish collected. No other species comprised more than 1% of the numerical catch.

Emerald Shiner and, to a lesser extent, Spotfin Shiner strongly dominated the catch at each location, comprising 91 to 97 percent of all fish collected. Location 5, near the mouth of South Kickapoo Creek, was the most productive of the three locations sampled in tenms of both species richness and the total number of fish collected (Table 4). For example, Location 5 yielded 906 fish comprised of 12 species compared to 65 to 245 fish comprised of three to six species at Locations 2 and 1, respectively. The better production at Location 5 is likely due to its proximity to South Kickapoo Creek, as well as habitat diversity and suitability of that location to seining.

Location 5 contains extensive shallow areas with clean sand, gravel, and cobble which is generally better seining habitat compared to upstream locations. Seven species were collected exclusively at Location 5, including several stream dwelling species such as Central Stoneroller, Silverjaw Minnow, and Suckermouth Minnow, which are likely waifs from South Kickapoo Creek (Table 4). In contrast, Emerald Shiner and Spotfin Shiner, the two most abundant species collected seining overall, were collected at all three sampling locations. They comprised 91 to 97 percent of the catch at each location.

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3..1.3 DELT Anomalies DELT anomalies are most relevant for this study because a clear relationship has been established between the incidence (percentage) of DELT anomalies and water quality (Ohio EPA 1989). Percent DELT anomalies were low among all fish collected by electrofishing and seining during the 2013 survey. For example, only two of the 1,295 fish collected (0.2 percent) exhibited DELT anomalies (Table 5). Two Freshwater Drum, one collected upstream of the plant's discharge and one downstream, were each afflicted with a single DELT anomaly (eroded fins), resulting in an overall affliction rate of 0.1 percent at Locations 1 and 2 (Table 5). No fish collected at Location 5 seine exhibited DELT anomalies. An increase in the affliction rate of DELT anomalies usually indicates stress and environmental degradation. The presence of deformities, erosions, lesions, and tumors can indicate environmental stress when a large percentage of the population is afflicted. The low incidence of DELT anomalies observed in the local fish community during this study does not suggest any obvious environmental stressors in this portion of the Illinois River.

No external parasites or other abnormalities were observed on any fish collected during the 2013 study.

3.1.4 Relative Weight Inherent in the development of standard weight (Ws) equations used to calculate relative weight (Wr) of fish is the objective of modeling the growth form of a species for individuals in better-than-average condition for management purposes. A mean Wr value close to 100 for a broad range of size groups may reflect optimal health and utilization of food resources for a given population (Anderson and Gutreuter 1983). Mean Wr values considerably less than 100 may suggest low food availability and/or disruption of feeding relationships or the presence of various environmental stressors. However, relative weights can also be influenced by several factors inclUding sex, fish size, and spawning condition.

In total, 53 fish comprised of 9 species that met the minimum length criteria for the Wr equations were collected in 2013 (Table 6). Only two of the nine species collected (Gizzard Shad and Smallmouth Buffalo) were represented by five or more specimens and will be the focus of this discussion. All remaining species were collected in such low numbers rendering those data less meaningful. The mean Wr for Gizzard Shad for locations combined was 91 and was slightly higher downstream of the plant's discharge (92) than upstream (89), whereas the mean Wr for Smallmouth Buffalo was 78, but again slightly higher downstream (80) compared to upstream (76). The mean relative weight of 78 observed for Smallmouth Buffalo is less than ideal,

• suggesting food availability and/or feeding relationships for this bottom feeding species may not be optimal in this portion of the Illinois River, at least for those few individuals that were collected in 2013 (Table 6).

3.2 BENTHOS During 2013, benthic macroinvertebrate sampling was conducted at four locations on the Illinois River that had been established during pre-operational studies for the LaSalle County Station 9

(Figure 1). HD samples were retrieved from three of four locations. Both sets of HD samplers that were deployed at Location 1 were missing upon retrieval and appeared to have been vandalized. However, Ponar samples were collected successfully at all four locations.

Overall, the benthos collections yielded 61 total taxa (Table 7). Chironomidae was the most taxa rich group with 21 taxa followed by Ephemeroptera and Bivalvia with eight taxa each.

3.2.1 H-ester-Dendy The three HD samples yielded a combined 31 total taxa (Table 8). Chironomidae were the most taxa rich group with 10 taxa while Ephemeroptera and Trichoptera each contributed five taxa to the total HID richness. Total taxa richness was highest among Locations 3 and 4 (23-25 taxa),

and slightly lower at Location 2 (Table 8). As with total taxa, EPT richness was highest among Locations 3and 4 (9-10 taxa) and lower at Location 2 with five taxa (Table 8).

Total density (no./m 2) was similar and substantially higher at Locations 3 and 4 compared to' Location 2 (Table 8). The higher densities observed at Location 3 was due to high numbers of Dicrotendipes spp., which composed 35 percent of the total density at Location 3 (Table 8). At Location 4, several taxa contributed to elevated density including Maccaffertium integrum, Cheumatopsyche, and Dicrotendipes spp. Overall, dominant taxa among the three locations exhibited some similarities but no longitudinal trends.

HD Dominant Taxa in Order of Percent Abundance Location 2 Location 3 Location 4 C. fraternus D. modestus Cheumatopsyche D. neomodestus Turbellaria D. simpsoni M. integrum D. modestus C. fraternus D. modestus Hydroptila Glyptotendipes Stenacron M. integrum Stenacron D. neomodestus Based on the environmental tolerance values developed by the Illinois Environmental Protection Agency (IEPA 1987; Tetra Tech 2007), the only tolerant taxon among the dominants was Glyptotendipes. In contrast, the remaining dominant taxa are considered facultative to slightly intolerant. Dicrotendipesmodestus was among the dominant taxa at each of the three locations while Maccaffertium integrum, Cyrnellusfraternus, and Dicrotendipes neomodestus were among the dominant taxa at two of the three locations.

3.2.2 Ponar and Kick Net Samples The Ponar grab samples from the four locations yielded 42 total taxa and seven EPT taxa (Table 9). Chironomidae was the most taxa rich group with 15 taxa while eight Bivalvia and four Ephemeroptera taxa were also observed. Total taxa richness was highest among Locations 1 and 3 (20-29 taxa) and noticeably lower at Locations 2 and 4 with 16 to17 taxa (Table 9). Despite the differences in total taxa richness, more pollution sensitive, EPT richness was generally similar among the locations, ranging from three to five taxa (Table 9).

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Total density (no./m 2 ) was highest at Location 3 while noticeably lower and similar at Locations 1, 2, and 4 (Table 9). The higher density observed at Location 3 was due almost exclusively to the elevated abundance of the nonindigenous sideswimmer, Apocorophium lacustre,.which accounted for 70 percent of the total abundance. Ponar distribution of abundance was relatively similar throughout the study area. Tubificidae and the nonindigenous Asiatic clam, Corbicula fluminea, were among the dominant taxa at each location while Cryptochironomus and Polypedilum halteralegrp. were among the dominants at three of the four locations.

Ponar Dominant Taxa in Order of Percent Abundance Location 1 Location 2 Location 3 Location 4 Tubificidae A. lacustre A. lacustre Tubificidae P. halterale grp. Tubificidae Tubificidae Corbiculafluminea Cryptochironomus Chironomus Cryptochironomus P. halterale grp.

Corbiculafluminea Corbiculafluminea Corbiculafluminea Cryptochironomus Elimia P. halterale grp. Caenis P. scalaenum grp.

C. fraternus Based on JEPA environmental tolerance values (IEPA 1987; Tetra Tech 2007), relatively tolerant taxa such as Tubificidae, Chironomus, and Cryptochironomus were abundant at all locations. Relatively intolerant or facultative taxa such as Polypedilum halterale grp. and Corbiculaflumineawere present at each location but were generally less abundant than more tolerant taxa.

Ponar substrate composition varied somewhat among the locations. Locations 1 and 4 had fewer substrate types with higher amounts of fine material and detritus. In contrast, Locations 2 and 3 had more diverse substrate composition that trended toward coarser material.

Ponar Percent Substrate Comp osition Substrate 1 2 3 4 Cobble 5 5 Gravel 5 45 45 15 Coarse Sand - 60 30 10 Fine Sand 20 10 20 15 Silt 10 40 Clay 5 Detritus 15 10 5 30 In the absence of water quality impacts, benthic community quality generally increases with habitat and substrate complexity. This corresponds with the Ponar results from Location 3, which had the highest total and EPT taxa richness as well as relatively more diverse substrate composition (Table 9). However, there was no consistent relationship between benthic community quality and substrate composition at the remaining three locations.

11

4. DISCUSSION 4.1 FISH Annual construction/preoperational biomonitoring studies were conducted in the Illinois River in areas uPstream and downstream of the LaSalle County Station's river intake screenhouse and discharge location from 1974-1978, prior to the station becoming operational in January and October 1984. In addition to the current 2013 study, post operational fish and benthic studies were conducted in 1999 (EA 2000). The 1999 study was conducted in response to an extended period of low water levels and high water and air temperatures. It was part of a broader upper Illinois River program which in some cases utilized slightly different sampling protocols. Where appropriate, results of the current study (i.e., fish condition, species composition, relative abundance, and catch rates) were compared to prior pre/post operational studies to determine if changes in the Illinois River have occurred that could be related to station operation.

4.1.1 Historical Operational Studies The 1999 study included five electrofishing surveys from July through October at three locations, one upstream of the discharge and two downstream of it. The two electrofishing locations sampled during the current study were similar to two of the three locations sampled in 1999, utilizing the same electrofishing gear. The third location sampled in 1999 was a far field zone established downstream of South Kickapoo Creek. All electrofishing zones were 500 m in length for both studies. For comparative purposes, only the results from the August 1999 survey for the two locations immediately upstream and downstream of the LaSalle County Station discharge were compared to the current August survey. Seining was not discussed as this gear was not utilized during the 1999 study.

Overall, species Composition was generally similar between August electrofishing surveys in 1999 and 2013, with'Gizzard Shad, Smallmouth Buffalo, and Freshwater Drum consistently among the top five most abundant species collected (EA 2000). These three species comprised 52 percent (1999) to 59 percent (2013) of the total catch for locations combined. In contrast, the relative abundance of some species, such as Spotfin Shiner and Bluegill, were quite different between years. For example, Spotfin Shiner was the second most abundant species collected at both locations in 2013, but was not collected upstream of the plant's discharge and was represented by less than 3 percent of the catch downstream of it in 1999 (EA 2000). Similarly, Bluegill represented 9 to 14.5 percent of the catch upstream and downstream of the plant, respectively, in 1999 but was uncommon upstream and absent downstream of the plant during the current study.

In total, 14 species were collected electrofishing at the two locations sampled in August 2013 compared to 19 in 1999 (EA 2000). The reduced species richness observed in 2013 was most pronounced among native minnows as five species were collected in 1999 compared to two during the current study. The August electrofishing survey in 1999 yielded 12 species upstream of the LaSalle County Station discharge and 16 species downstream, compared to 13 and nine species collected during the current study, respectively. Differences in species richness between study years, particularly downstream of the station discharge, were typically associated with 12

incidental catches of less commofi species such as Rosyface Shiner, Bullhead minnow, Quillback, Black Buffalo, Rock Bass, and Black Crappie; all of which were represented by a single specimen in 1999.

The total number of fish collected at each 500 m zone was very similar spatially within a given year, but considerably higher in 1999 compared to 2013. For example, 75 fish were collected upstream of the plant's discharge in 1999 compared to 76 downstream, whereas 36 fish were collected upstream in 2013 compared to 43 downstream (EA 2000). Gizzard Shad dominated the catch at both locations in 2013, whereas Gizzard Shad and Smallmouth Buffalo dominated the catch upstream and downstream, respectively, in 1999. The higher catches observed in 1999 were attributable to increased numbers of most species encountered (e.g., Gizzard Shad

[primarily upstream], Smallmouth Buffalo, Green Sunfish, Bluegill, Freshwater Drum, and Channel Catfish).

No species listed as endangered or threatened by Illinois were observed during operational fish surveys (IiESPB 2011).

The overall affliction rate of DELT anomalies was much lower in 2013 (0.2 percent) compared to 1999 (6.6 percent). Furthermore, only one species collected in 2013 (Freshwater Drum) exhibited a DELT anomaly compared to the affliction of 13 species in 1999 (EA 2000,). The highest affliction rates in 1999 were among bottom feeding species such as Channel Catfish, Black Buffalo, River Carpsucker, Freshwater Drum, and Common Carp.

4.1.2 Pre-Operational Studies Construction/preoperational baseline biomonitoring studies were conducted annually from 1974-1978 near the LaSalle County Station (Bio-Test 1975; Nalco 1976, 1977, 1978; and Hazelton 1979). Seasonal fish sampling was conducted by seining (five locations) and electrofishing (two locations) in February (seining only), May, August, and November. Two of the five seine locations were Illinois River locations (one upstream and one downstream of the plant's discharge) and three were located within South Kickapoo Creek (SKC), including one near the confluence with the Illinois River. Three of the five seine locations and both electrofishing locations were the same as those sampled in 2013. Each electrofishing location was sampled for 500 m, utilizing a 230-V, 3-phase AC generator. Seine samples were collected using a 25-ft x 6-ft seine with 1/4-inch mesh by making two or three hauls at each river location and one haul at each creek location. Electrofishing samples were collected on four consecutive days (four samples collected) each survey period, whereas seine samples were collected on two consecutive days at the three SKC locations and four consecutive days at the two Illinois River locations. K-factors were determined by species for immature and adult fish. All fish collected were examined for external disease, parasitism, and abnormalities.

As indicated below, the combined data for all gear types show that species richness in the Illinois River and SKC was generally comparable during the five year study period:

13

Study Year Overall 197.._4 197_.55 197._66 1977 197._88

1. Fish 6,638 3,068 7,681 10,146 4,260

1. Species 43 42 41 41 36 IL River

1. Fish 2,172 1,716 5,990 6,627 2,036

1. Species 29 32 30 34 29 S. Kick. Crk.

1. Fish 4,466 1,352 1,691 3,519 2,224

1. Species 30 27 27 28 23 The combined data for all gear types also show that overall (Illinois River and SKC combined) species richness ranged from 36 in 1978 to 43 in 1974. Temporally, species richness varied only slightly within each study area ranging from 29 to 34 within the Illinois River and 23 to 30 in SKC. Species richness was typically higher in the Illinois River compared to SKC within a given year. Total numbers of fish collected by electrofishing and seining during the five year period varied moderately from year to year. Numbers of fish were generally comparable in 1974, 1975, 1976, and 1978 (range = 3,068 to 7,681) and moderately higher in 1977 when over 10,000 fish were collected. Species composition was equally consistent among years within the Illinois River.* For example, Emerald shiner, Gizzard Shad, and Common Carp were consistently among the four most abundant species collected each year from 1974 through 1978.

Collectively, these three species comprised 86 to 94 percent of the annual catch from the illinois River. Species dominance within SKC was not nearly as consistent on a year to year basis.

Emerald Shiner, Creek Chub, and Silver Jaw Minnow were the most abundant species collected in SKC, collectively comprising 47 to 64 percent of the total catch on an annual basis. Other abundant species collected within SKC included Central Stoneroller, Bluntnose Minnow, White Sucker, Southern Redbelly Dace, and Green Sunfish.

Species richness has remained nearly constant among the two electrofishing locations combined, varying from 23 to 25 from 1974 through 1978. By location, 12 to 21 species were collected annually upstream compared to 17 to 22 species collected downstream. Annual mean electrofishing CPEs (# fish/hour) were comparable upstream of the plant (range = 59-168) and downstream (52-153) during the five years of studies and were generally higher in 1975 and 1977 in both areas. The higher CPEs observed in 1975 and 1977 were due to higher catches of the most common species, i.e. Emerald Shiners and Gizzard Shad. Seasonally, CPEs at both" locations were generally highest in August and lower in May and November, except in 1978 when CPEs were highest in November.

Emerald Shiner was the most abundant species collected seining at Locations 1 and 2 in the Illinois River from 1974 through 1978, comprising 72 to 99 percent of the catch. Over the five year period, Location 1, upstream of the plant's discharge, yielded 8 to 17 species compared to 7 to 21 species collected downstream. As expected, species richness was more variable among the three SKC locations from 1974 through 1978, where 19 to 24 species were collected at the downstream most location (near mouth), 15 to 23 at the middle reach, and 4 to 12 at the furthest 14

upstream location. Similarly, dominant species varied from year to year as well. For example, Emerald Shiner consistently dominated the seine catch near the mouth, but was generally uncommon from the middle reach, and was absent upstream, whereas Creek Chub, Silverjaw Minnow, Bluntnose Minnow, and Fathead Minnow dominated the catches at the middle and upper locations from 1974 through 1978. The observed differences in species richness and species composition at the three SKC locations is likely attributable to stream size and habitat quality. In wadable streams, there is a well-established, direct relationship between stream size and species richness (OEPA 1987). Thus, one would expect fewer species at those locations with smaller drainage areas (e.g., headwater sites) and more species at downstream locations. Thus, the lower number of species collected within the upper reach in SKC (range

= 4 to 12) compared to that near the mouth (19 to 24), is likely due, in part, to stream size and proximity to the Illinois River.

Condition factors (K) were determined for individuals of 13 species collected seasonally from the river and creek stations from 1974 through 1978. Similar to relative weight (Wr) calculation used in the operational studies, differences in values can be caused by a variety of environmental and biological factors (e.g., food availability and standing crop). Changes in condition can indicate that a factor or combination of factors have altered the growth characteristics of an individual. Condition factors were reported by season and by life stage (i.e., juveniles and adults). Condition factors were similar between years among species collected in sufficient numbers to reflect a reliable index and were not statistically different upstream and downstream of the plant's discharge. In August, K Factors among the most abundant species ranged from 1.45 to 1.62 for Common Carp, 1.00 to 1.24 for Gizzard Shad, and 2.15 to 2.28 for Green Sunfish. These values are in the range reported in the literature (Carlander 1969 and 1977).

The incidence of external parasites, disease, and physical abnormalities was assessed on all fish collected during the five year period from 1974 through 1978. Blackspot was the most common external parasite observed during the study period. External parasites were most common among Emerald Shiners and Green Sunfish. Exophthalmus (popeye) was a cOmmon disease among goldfish upstream and downstream of the plant in 1974 and 1975, and upstream of the plant in 1978 where 27 percent of the goldfish were afflicted. This disease was not observed in 1976 or 1977. Deformed and eroded fins were most common physical abnormalities observed during the

• five year period. Affliction rates of deformed and eroded fins were highest among Common Carp.

4.1.3 Summary The fish community in the Illinois River is comprised of a variety of forage, game/sport, and commercial/rough fish as would be expected in a Midwestern river of its Size. The preoperational and operational fish assemblages were generally similar as they shared forage fish such as Emerald Shiner, Spotfin Shiner, Spottail Shiner, and Gizzard Shad; game species such as Channel Catfish, Bluegill, Green Sunfish, Smalimouth Bass, and Largemouth Bass; and rough/commercial species such as Common Carp, Smallmouth Buffalo, and redhorse species.

The dominant species collected in the Illinois River (i.e., Gizzard Shad, Emerald Shiner, Smalimouth Buffalo, and Freshwater Drum) were consistent during preoperational and operational studies 15

Fish species richness was comparable for the preoperational sampling period (1974 to 1978) and operational sampling periods (1999 and 2013). For example, during the 1974 to 1978 baseline studies, electrofishing species richness ranged from 16 to 19 species in August, compared to 19 species in 1999 and 14 species in 2013. Diversity among species collected seining was slightly lower, but comparable, as six species were collected upstream of the plant's discharge and three to 12 species/location downstream in 2013 compared to 8 to 17 species/location upstream and 7 to 21 species/location downstream from 1974 to 1978. The slightly higher species richness values observed during the baseline studies is likely due to the increased level of effort during those studies, which included four sampling passes over four days at each seine and electrofishing zone in the Illinois River, compared to the more typical 2013 study when only a single pass was conducted.

The mean operational electrofishing CPEs (# fish/hour) in 2013 were similar upstream (74) and downstream (86) of the plant and were in range of preoperational values observed upstream (59 to 168) and downstream (52 to 153) from 1974 through 1978. CPEs were not discussed for the 1999 study because they were calculated based on distance (# fish/kin) rather than time (#

fish/hour) as was done during the preoperational studies and the current study.

The percent DELT anomalies was lower in 2013 (0.2 percent) compared to 1999 (6.6 percent).

Only Freshwater Drum exhibited DELT anomalies compared to 13 species in 1999. External parasites and disease were not observed on fish collected during the current study. During preoperational studies, blackspot was the most common external parasite observed; Exophthalmus (popeye) was a common disease among goldfish in 1974 and 1975, and upstream of the plant in 1978; and deformed and eroded fins were most common physical abnormalities observed during the five year period.

No species on the Illinois list of endangered and threatened species have been reported from the preoperational and operational studies near the LaSalle County Station discussed herein.

Collectively, these data suggest that the operation of the LaSalle County Station has not impacted the resident fish populations near the plant in a negative manner. It is reasonable to expect that if the operation of the LaSalle County Station was adversely affecting fish populations, then it would be manifested in comparatively lower catches, significantly reduced species diversity, and/or increased affliction of external anomalies during the operational studies conducted near, and particularly downstream of, the plant; these trends have not been apparent.

The NPDES permit for LaSalle County Station was renewed in July 2013 by the JEPA. After agency review of the renewal application and other data available to agency staff, it was determined that LaSalle County Station, as operated, was in compliance with environmental regulations, in particular thermal and discharge issues. Special Condition 3 states, "This facility

[LaS alle] meets the criteria for establishment of a formal mixing zone for thermal discharges pursuant to 35 1AC 302.102" and proceeds to define the parameters for the LaSalle mixing zone, which are the State's standard. Special Condition 15 states:

"The facility utilizes a closed-cycle recirculating cooling system, a 2058 acre 16

cooling pond, for cooling of plant condensers and is determined to be the equivalent of Best Technology Available (BTA) for cooling water intake structures to prevent/minimize impingement mortality in accordance with the Best Professional Judgment (BPJ) provisions of 40 CFR 125.3 because it allows the facility to only withdraw the amount of water necessary to maintain the cooling pond level rather than the entire volume used for cooling of the plant condensers.

In order for the Agency to evaluate the potential impacts of cooling water intake structure operations pursuant to 40 CFR 125.90(b), the permittee. shall prepare and submit information to the Agency outlining current intake structure conditions at this facility, including a detailed description of the current intake structure operation and design, description of any operational or structural

• modifications from original design parameters, source waterbody flow information as necessary.

'* The infornation shall also include a summary of historical 316(b) related intake impingement and/or entrainment studies, if any, as well as current impingement mortality and/or entrainment characterization data; and shall be submitted to the Agency within six (6) months of the permit's effective date.

Upon the receipt and review of this information, the permit may be modified to require the submittal of additional information based on a Best Professional Judgment review by the Agency. This permit may also be revised or modified in accordance with any laws, regulations, or judicial orders pursuant to Section 316(b) of the Clean Water Act".

LaSalle County Station is conducting a bi-weekly impingement study of the make-up water intake. In addition, a study to assess entrainment of the make-up water intake will begin in April 2014. The two studies are anticipated to be completed within the first quarter of 2015 and the results will be available in the second quarter of 2015.

4.2 BENTHOS As with the fisheries studies, benthic sampling was a component of the pre-operational aquatic monitoring program that was conducted from 1974 to 1978 (Bio-Test 1975; Nalco 1976, 1977,*

and 1978; and Hazelton 1979). In addition, the benthic macroinvertebrate community was assessed as part of studies conducted near the LaSalle County Station in 1999 (EA 2000). The most *recent year of analysis was 2013 and those results are presented in Section 3 of this report.

Although the pre-operational and operational studies have differed in terms of effort, duration, and taxonomic treatment, the Sampling methods (i.e., HD artificial substrates and/or Ponar dredge) and locations were similar among survey years.

4.2.1 Historical Operational Studies In addition to the 2013 benthic survey, one previous operational benthic macroinvertebrate survey has been conducted in 1999 near LaSalle County Station (EA 2000). As part of this 17

survey, duplicate Ponar grabs of 523 cm 2 each were collected from Locations 1, 2, and 4 (Figure 1). Benthic sampling was conducted once in August (Table 10), twice in September, and once in October. The Ponar samples were processed in the same manner as described in Section 2 for the 2013 survey.

During the 1999 surveys, 34 total taxa were observed (EA 2000). Chironomidae was the most taxa rich group with 12 taxa. Total taxa richness for the combined locations was similar among the sampling events. Spatially, taxa richness was higher downstream of the LaS alle Station discharge at Locations 2 and 4 during each sampling event and for the trips combined. In August, both total and EPT richness were slightly higher at Locations 2 and 4 compared to Location 1 (Table 10).

Density (#/m 2) was highest in August and similar among the three later sampling events (EA 2000). Density was consistently higher downstream of the LaSalle Station discharge at Locations 2 and 4 during each sampling event and for trips combined. In August, density was noticeably higher at Locations 2 and 4 compared to Location 1 (Table 10). At all locations and during each sampling event, the benthic community was dominated by Oligochaeta and Chironomidae.

Results of the 1999 surveys indicated that the benthic community near the LaSalle Station is comparable to other areas on the Illinois River but generally of poor quality. The benthic community was dominated by relatively tolerant taxa at all locations and during each sampling event. Overall, no discernable spatial or temporal trends were consistently observed that suggest the benthic community was adversely affected by LaSalle County Station.

4.2.2 Pre-Operational Studies Pre-operational benthic macroinvertebrate studies conducted from 1974 to 1978 included Ponar and HD sample collections from Locations 1, 2, 3, and 4 on the Illinois River (Figure 1) as well as qualitative dip net collections at two locations in South Kickapoo Creek (Bio-Test 1975; Nalco 1976, 1977, and 1978; and Hazelton 1979). Sampling was conducted in February, May, August, and November of each year. With some exceptions, four Ponar grabs (530 cm2 each) and four HD samplers (0.05 m2 each) were collected during each sampling event from the Illinois River locations along with qualitative dip net samples from South Kickapoo Creek. As described in the reports, HD sampler loss or other factors prevented successful HD and/or Ponar sampling at various locations during the five years of study. However, these occurrences were generally uncommon.

Despite seasonal and annual differences for specific parameters, general trends in the benthic macroinvertebrate community were notably similar over the five study years. Total taxa richness for all locations and sample types combined ranged from 100 taxa in 1978 to 156 taxa in 1977 (Bio-Test 1975; Nalco 1976, 1977, and 1978; and Hazelton 1979). Environmentally tolerant Oligochaeta and Chironomidae consistently were the most taxa rich and numerically abundant in the HD and Ponar samples. Environmentally intolerant EPT taxa richness ranged from 12 taxa in 1974 and 1978 to 21 taxa in 1975. However, most EPT taxa were observed in the South Kickapoo Creek collections and, except for the seasonal occurrence of one or two taxa, generally 18

represented less than five percent of the total abundance in the Illinois River HD and Ponar samples.

HD total taxa richness varied annually and seasonally among locations but some trends were evident. In four of the five years of study, total taxa richness among the four locations was lowest in February and highest in August (Table 11). Spatially, total richness was highest at Location 3 during 12 of 20 sampling events and lowest at Location 4 in 11 of 20 sampling events. Totaltaxa richness was consistently lower at Location 4 in 1977 and 1978. Road construction activities and a washout of a township road crossing over South Kickapoo Creek occurred in 1976 and 1977 (Nalco 1978 and Hazelton 1979). These events resulted in increased bedload movement and deposition of fine sediment at Location 4 and likely contributed to lower taxa richness in 1977 and 1978.

As with HD taxa richness, density varied annually and seasonally among locations. However, HD density was consistently lower in February each year and highest in August for four of the five study years (Table 11). The low densities in February were attributed to cool water temperatures and the corresponding effects on benthic macroinvertebrate activity, such as drift, emergence, and recruitment (Bio-Test 1975; Nalco 1976, 1977, and 1978; and Hazelton 1979).

Spatially, density was slightly more variable compared to taxa richness. Nonetheless, spatial trends for the two parameters were similar with density being highest at Location 3 for 10 of 20 surveys and lowest at Location 4 during nine of 20 sampling events. With few exceptions, Chironomidae were generally the dominant group in terms of abundance and regardless of study year, sampling event, or location.

Ponar and dip net (i.e., natural substrate) total taxa richness also varied annually and seasonally among locations. However, as was observed in the HD results, the natural substrate data presented consistent trends. In each of the five years of study, total taxa richness among the four Illinois River locations and two South Kickapoo Creek locations was higher in February and lower in either May or November (Table 12). Spatially, total richness was highest at Location 4 during 11 of 20 sampling events and lowest at Location 1 in 12 of 20 sampling events.

Density in the natural substrate samples was clearly more variable than taxa richness for either sample type as well as HD density. In three of five study years, density was highest in February, however, none of the four sampling periods consistently produced the lowest density (Table 12).

The higher densities in February were attributable to the increased abundance of.Oligochaeta and often appeared to correspond with higher flow conditions (Bio-Test 1975; Nalco 1976, 1977, and 1978; and Hazelton 1979). Oligochaeta abundance typically decreased each year from February to November. Among locations, density was lowest at Location 1 for seven of the 20 sampling events, which was consistent with total taxa richness for the natural substrate samples. However, there was no corresponding spatial relationship with highest density at Location 3 and taxa richness. Temporally and spatially, Oligochaeta and Chironomidae were generally the dominant groups in the natural substrate samples with some variability associated with substrate composition among locations.

Overall, the five Years of pre-operational surveys indicate that the Illinois River benthic macroinvertebrate community was composed of facultative and environmentally tolerant 19

organisms; not atypical of a large river in the immediate, post-Clean Water Act era. Although seasonal and spatial trends were evident, the patterns observed in the benthic community were attributable to natural seasonal variation and/or substrate composition. None of the trends

'observed were related to construction activities associated with the LaSalle County Station 4.2.3 Summary Comparisons between the pre-operational and operational benthos surveys are challenging due largely to variations in sampling methods such as different types of modified HDs (nine round plates 0.05 m2 [rarely used now] versus five square plates 0.093 in 2), number of sampling events, and differences in taxonomic resolution among studies. In addition, the effects of inherent changes that have occurred in the Illinois River and watershed (e.g., land use, introduction of exotic species, etc.) over nearly 40 years are not completely understood. Despite these differences and challenges, the pre-operational, 1999, and 2013 results exhibited some similarities.

For the August HD results, Chironomidae was the dominant group in terms of richness and a~bundance in both the pre-operational and 2013 studies (Bio-Test 1975; Nalco 1976, 1977, and 1978; Hazelton 1979; and Table 8). In addition, Dicrotendipeswas among the most common taxa identified from HD samples collected during the 1974-1978 pre-operational surveys and was the most abundant Chironomidae taxa in each of the three HD locations in 2013.

Pre-operational HD taxa richness for the five August sampling events ranged from 13 taxa at Location 4 in 1977 to 34 taxa at Location 1 in 1975 with an average August richness of 25 taxa for the five years combined (Table 11). In comparison, HD taxa richness among the three August 2013 operational samples ranged from 18 taxa at Location 2 to 25 taxa at Location 4 (Table 8). Given that the pre-operational assessments identified Oligochaeta to genus/species level while the 2013 surveys treated Oligochaeta as a single taxon, the 2013 HD taxa richness was clearly similar to or higher than historical results.

Pre-operational HD density for the five August sampling events ranged from 1,285/m 2 at Location 4 in 1976 to 13,410/in 2 at Location 3 in 1977 with an average August density of 4,329/in 2 for the five years combined (Table 11). In comparison, HD density among the three August 2013 stations was similar to the pre-operational results and ranged from 1,546/m 2 at Location 2 to 5,246/in 2 at Location 3 (Table 8).

Among the August Ponar results, Oligochaeta and Chironomidae were among the two most abundant groups during both pre-operational and operational studies (Bio-Test 1975; Nalco 1976, 1977, and 1978; Hazelton 1979; Tables 9 and 10). Specifically, Tubificidae, Cryptochironomus, and Polypedilum spp. were among the dominant taxa observed at the Illinois River locations during August pre-operational and operational study periods.

Pre-operational Ponar taxa richness for the five August sampling events and four Illinois River locations ranged from seven taxa at Locations 1 and 2 in 1975 to 26 taxa at Location 4in 1975 with an average August richness of 12 taxa for the five years combined (Table 12). Ponar taxa richness among the three August 1999 operational samples ranged from 10 taxa at Location 1 to 20

12 taxa at both Locations 2 and 3 (Table 10). Ponar taxa richness among the four August 2013 operational samples ranged from 16 taxa at Location 2 to 29 taxa at Location 3 (Table 9). Given that the pre-operational assessments and the 1999 operational surveys identified Oligochaeta to genus/species level while the 2013 study treated Oligochaeta as a single taxon, the 2013 Ponar taxa richness was clearly similar to or higher than historical results.

Pre-operational Ponar density for the five August sampling events and four Illinois River locations ranged from 119/mn2 at Location 1 in 1975 to 5,741/in2 at Location 4 in August 1977 with an average August density of 1,302/ni 2 (Table 12). Ponar density among the three August 1999 operational samples ranged from 698/mi2 at Location 1 to 2,095/in 2 at Location 2 (Table 10). In comparison, August 2013 Ponar density ranged from 608/nm2 at Location 1 to 2,607/mi2 at Location 3; within the range observed during the pre-operational surveys and nearly identical to the 1999 results (Table 9).

Although some differences in the benthic community were evident among study years, overall, the 2013 benthic community in the Illinois River near the LaS alle County Station was rather similar to the pre-operational and historic operational benthic community. Community structure remained generally consistent as evidenced by the dominant taxa among studies. In addition, trends observed nearly 40 years ago were evident in the 2013 collections. In both surveys, longitudinal changes appeared to be partly due to differences in substrate composition rather than construction or operation of the LaSalle County Station. Just as the 1974-1978 and 1999 benthic surveys showed no effects related to pre-operational and operational activities, the 2013 survey supports these historical data in demonstrating no operational effects.

21

5. REFERENCES Anderson, R.O. and S.J. Gutreuter. 1983. Length,.Weight, and Associated Structural Indices.

Pages 283-300. In Nielsen, L.A. and D.L. Johnson (Eds.). Fisheries Techniques.

Southern Printing Company, Inc., Blacksburg, VA.

Anderson, R.O. and R.M. Neumann. 1996. Length, Weight, and Associated Structural Indices.

Pages 447-482. In (Murphy, B.R. and D.W. Willis, Eds.) Fisheries Techniques, 2 nd Edition. Am. Fish. Soc., Bethesda, MD.

Baumann, P.C., W.D. Smith, and W.K. Parland. 1987. Tumor frequencies and contaminant

• concentrations in brown bullhead from an industrialized river and a recreational lake.

Trans. Am. Fish. Soc. 116(1):79-86.

Becker, G.C. 1983. Fishes in Wisconsin. The University of Wisconsin Press. Madison, WI.

Industrial Bio-Test Laboratories, Inc. (Bio-Test). 1975. Aquatic Monitoring Program for the Construction Phase of the LaSalle County Station, 1974. Prepared for Commonwealth Edison Company, Chicago, IL.

Bister, T.J., D.W. Willis, M.L. Brown. 2000. Proposed standard weight (Ws) equations and standard length categories for 18 warmwater nongame and riverine fish species. N. Am.

Jour. Fish. Mgt. 20:570-574.

Carlander, K.D. 1969. Handbook of Freshwater Fishery Biology, Vol. 1. Iowa State University Press, Ames.

_______1977. Handbook of freshwater fishery biology, Volume 2. The Iowa State University Press, Ames, Iowa, USA.

EA Engineering, Science, & Technology, Inc. 2000. Final Report Lasalle Station Aquatic Monitoring 1999, Rm 249.7-248.8. Prepared for Commonwealth Edison Company, Chicago, IL.

Hazelton Environmental Sciences Corporation (Hazelton). 1979. Aquatic Monitoring Program for the Construction Phase of the LaSalle County Station, 1978. Prepared for Commonwealth Edison Company, Chicago, IL.

Illinois Endangered Species Protection Board (IESPB). 2011.. Checklist of Endangered and Threatened Animals and Plants of Illinois. Effective 11 February 2011. Illinois Endangered Species Protection Board, Springfield, IL.

http://www.dnr.illinois.gov/ESPB/Documents/ETChecklist20 11 .pdf Illinois Environmental Protection Agency (IEPA). 1987. Field and Laboratory Methods Manual: Section C, Macroinvertebrate Monitoring. IEPA. Springfield, IL. 55 pp.

22

Murphy, B.R., D.W. Willis, and T.A. Springer. 1991. The relative weight index in fisheries management: status and needs. Fisheries 16(2): 30-28.

Nalco Environmental* Sciences (Nalco). 1976. *Aquatic Monitoring Program for the Construction Phase of the LaSalle County Station, 1975. Prepared for Commonwealth Edison Company, Chicago, IL.

________*1977. Aquatic Monitoring Program for the Construction Phase of the LaSalle County Station, 1976. Prepared for Commonwealth Edison Company, Chicago, IL.

______*1978. Aquatic Monitoring Program for the Construction Phase of the LaSalle County Station, 1977. Prepared for Commonwealth Edison Company, Chicago, IL.

Ohio Environmental Protection Agency. 1987 (Updated November 8, 2006). Biological criteria for the protection of aquatic life: Vol. II. Users manual for biological field assessment of Ohio surface waters. Div. Water Quality Monitoring and Assess., Surface Water Sect.,

Columbus, OH.

Ohio Environmental Protection Agency. 1989 (Updated November 8, 2006). Biological criteria for the protection of aquatic *life: Vol. III. Standardized field and laboratory methods for assessing fish and macroinvertebrate communities. Div. Water Quality Monitoring and Assess., Surface Water Sect., Columbus, OH.

Pflieger, W.L. 1975. The Fishes of Missouri. Missouri Department of Conservation. Jefferson City, Missouri.

Post, G. 1983. Textbook of fish health. TFH Publication, Inc. Neptune City. 256 pp.

Smith, P. 1979. The Fishes of Illinois. Univ. of Illinois Press, Urbana, IL.

Tetra Tech, Inc. 2007. Illinois Benthic Macroinvertebrate Collection Method Comparison and Stream Condition Index Revision, March 2007. Prepared for JEPA, Springfield, IL. 122 pp.

Trautman, M.B. 1981. The Fishes of Ohio. Ohio State Univ. Press, Columbus. 782 pp.

Wege, G.J. and R.O. Anderson. 1978. Relative weight (W,.): A new index of condition for largemouth bass. Pages 79-9 1 in Novinger G.D. and J.G. Dillard, eds. New approaches to the management of small impoundments. North Central Division, Am. Fish. Soc.,

Special Publication 5.

23

FIGURES Marseilles LeQend 0 Hester-Dendy Sample Location

[] Ponar Sample Location A Seine Sample Location

• 0Etectrofishing Zone

'//°s IRiiVer A LOCATION 4 A LCTOLOCATION 3- [Discharge 0

.,,ooCreek . .. -° '

Co LOCATION 2 0 500 1,000 Feet February, 2014 LaSalle County Station Marseilles, Illinois Figure 1 2013 LaSalle County Station Fish and Benthos Monitoring

TABLES Table 1. Intercept (a) and Slope (b) Parameters for Standard Weight (Ws) Equations with Minimum Total LenQths (mm) Recommended for Applicatior~a).

Minimum Slope (b) Length Reference or developer Species Intercept (a)

-6.8 11 3.449 200 Bister et al. (2000)

Longnose gar Gizzard shad -5.376 3.170 180 Anderson and Gutreuter (1983)

Rainbow trout (lentic) -4.898 2.990 120 Simpkins and Hubert (unpublished)

Brook trout -5.085 3.043 130 Whelan and Taylor (1984)

Chinook salmon -4.661 2.901 200 Halseth et al. (1990)

Northern pike -5.437 3.096 100 Willis (unpublished)

Common carp -4.639 2.920 2O00 Bister et al. (2000)

Golden shiner -5.593 3.302 50 Liao et al. (1995)

Bigmouth buffalo -5.069 3.118 150 Bister et al. (2000)

Smallmouth buffalo -5.298 3.208 200 Bister et al. (2000)*

River carpsucker -4.839 2.992 130 Bister et al. (2000)

White sucker -4.755 2.940 100 Bister et al. (2000)

Shorthead redhorse -4.841 2.962 100 Bister et al. (2000)

Black bullhead -4.974 3.085 130 Bister et al. (2000)

Yellow bullhead -5.374 3.232 60 Bister et al. (2000)

Brown bullhead -5.076 3.105 130 Bister et al. (2000)

Channel catfish -5.800 3.294 70 Brown et al. (1995)

Flathead catfish -5.542 3.230 130 Bister et al. (2000)

White perch -5.122 3.136 80 Bister et al. (2000)

White bass -5.066 3.081 115 Brown and Murphy (1991)

Yellow bass -5.142 3.133 70 Bister et al. (2000)

Striped bass -4.924 3.007 150 Brown and Murphy (1991)

Hybrid Morone -5.201 3.139 115 Brown and Murphy (1991)

Rock bass " -4.827 3.074 80 Bister et al. (2000)

Green sunfish -4.915 3.101 60 Bister et al. (2000)

Pumpkinseed -5.179 3.237 50 Liao et al. (1995)

Warmouth -5.180 3.241 80 Bister et al. (2000)

Bluegill -5.374 3.316 80 Hillman (1982)

Largemouth bass -5.316 3.191 150 Wege and Angerson (1978)

Smallmouth bass -5.329 3.200 150 Kolander et a1. (1993)

Black crappie -5.618 3.345 100 Neumann and Murphy (1991)

White crappie -5.642 3.332 100 Neumann and Murphy (1991)

Sauger -5.492 3.187 70 C.S. Guy (unpublished)

Walleye -5.453 3.180 150 Murphy et al. (1990)

Yellow perch -5.386 3.230 100 Willis et al. (1991)

Freshwater drum -5.419 3.204 100 Blackwell et al. (1995)

(a) Sources: Bister et al. 2000, Anderson and Neumann 1996, and Murphy et al. 1991.

Table 2. List of Common and Scientific Names of Fish Taxa Collected near LaSalle Station, August 2013.

COMMON FAMILY NAME COMMON NAME SCIENTIFIC NAME HERRINGS GIZZARD SHAD *Dorosoma cepedianum CARPS AND MINNOWS CENTRAL STONEROLLER Campostoma anomalum SPOTFIN SHINER Cyprinella spiloptera COMMON CARP Cyprinus carpio EMERALD SHINER Notropis atherinoides SILVER JAW MINNOW Notropis buccatus BIGMOUTH SHINER Notropis dorsalis SPOTTAIL SHINER Notropis hudsonius ROSYFACE SHINER Notropis rubellus SAND SHINER Notropis stramineus Notropis sp. Notropis sp.

SUCKERMOUTH MINNOW Phenacobius mirabilis BLUNTNOSE MINNOW Pimephales notatus BULLHEAD MINNOW Pimephales vigilax SUCKERS SMALLMOUTH BUFFALO Ictiobus bubalus GOLDEN REDHORSE Moxostoma erythrurum SHORTHEAD REDHORSE Moxostoma macrolepidotum NORTH AMERICAN CATFISHES CHANNEL CATFISH Ictalurus punctatus NEW WORLD SILVERSIDES BROOK SILVERSIDE Labidesthes sicculus SUNFISHES GREEN SUNFISH Lepomis cyanellus BLUEGILL Lepomis macrochirus SMALLMOUTH BASS Micropterus dolomieu LARGEMOUTH BASS Micropterus salmoides DRUMSANDCROAKERS DRUMS AD CROAKRS AplESnotusErunnieM Aplodinotus grunniens

Table 3. Number, Biomass, and Relative Abundance of Fish Collected near.LaSalle Station, August 2013.

ELECTRO FISHING SEINE GEARS COMBINED Minimum Maximum SPECIES KG KG  %/ KG  %/ Length (mm) Length (mm)

GIZZARD SHAD . 38 48.1 4.283 22.5 9 0.7 0.096 4.4 47 3.6 4.379 20.7 86 345 CENTRAL STONEROLLER 1 1.3 0.002 0.0 2 0.2 0.004 0.2 3 0.2 0.006 0.0 -- --

COMMON CARP 1 1.3 2.100 11.1 .. .. .. -.. 1 0.1 2.100 9.9 550 550 SILVERJAW MINNOW .. .. .. ..- .4 0.3 0.005 0.2 4 0.3 0.005 0.0 ....-

EMERALD SHINER 2 2.5 0.004 0.0 1,010 83.1 1.876 85.4 1,012 78.1 1.880 8.9 ....-

BIGMOUTH SHINER .. .. ... ..- 2 0.2 0.001 0 2 0.2 0.001 0.0 ....-

SPOTTAIL SHINER .. ..- -- -- 1 0.1 0.002. 0.1 1 0.1 0.002 0.0 ....-

ROSYFACE SHINER .. .. .. -.. 2 0.2 0.001 0 2 0.2 0.001 0.0 ....-

SPOTFIN SHINER 13 16.5 0.023 0.1 115 9.5 0.064 2.9 128 9.9 0.087 0.4 ....-

SAND SHINER .. .. .. -.. 21 1.7 0.017 0.8 21 1.6 0.017 .0.1 .....

Notropis sp. -- . .. .. .. 3 0.2 0.001 0 3 0.2 0.001 0.0 ....-

SUCKERMOUTH MINNOW .. .. .. -.. 2 0.2 0.003 0.1 2 0.2 0.003 0.0 ....-

BLUNTNOSE MINNOW .. .. .. -.. 27 2.2 0.038 1.7 27 2.1 0.038 0.2 ....

BULLHEAD MINNOW .. .. .. .. "- 9 0.7 0.003 0.1 9 0.7 0.003 0.0 ....-

SMALLMOUTH BUFFALO 5 6.3 4.185 22.0 .. .. .. - .. 5 0.4 4.185 19.7 356 430 GOLDEN REDHORSE 4 5.1 1.221 6.4 .. .. .. - .. 4 0.3 1.221 5.8 200 390 SHORTHEAD REDHORSE 2 2.5 0.850 4.5 .. .. .. -.. 2 0.2 0.850 4.0 355 385 CHANNEL CATFISH 2 2.5 1.600 8.4 .. .. .. ...- 2 0.2 1.600 7.5 365 500 BROOK SILVERSIDE .. .. .. -.. 8 0.7 0.006 0.3 8 0.6 0.006 0.0 ....-

GREEN SUNFISH 1 1.3 0.004 0.0 .. .. .. - .. 1 0.1 0.004 0.0 55 55 BLUEGILL 2 2.5 0.028 0.1 .. .. .. -.. 2 0.2 0.028 0.1 88. 90 SMALLMOUTH BASS 3 3.8 0.324 1.7 1 0.1 0.079 3.6 4 .0.3 0.403 1.9 145 242 LARGEMOUTH BASS 2 2.5 0.560 2.9 .. .. .. -.. 2 0.2 0.560 2.6 274 288 FRESHWATER DRUM . 3 3.8 3.820 20.1 .. .. .. -.. 3 0.2 3.820 18.0 435 512 TOTAL FISH TOTAL SPECIES 79 1 I100 119.004 1I 100 14 I1,216 1j 100 1 2.196 1I 100 14 I1,295 1 I100

.23 1I21.200 1I 100 NOTE: 0.0 indicates less than 0.05

Table 4. Fish Captured at each Electrofishing and Seining Location near LaSalle Station, August 2013.

ELECTROFISHING 1 2 Locations Combined SPECIES No. %No. %No.%

GIZZARD SHAD 10 27.8 28 65.1 38 48.1 CENTRAL STONEROLLER 1 2.8 1 1.3 COMMON CARP 1 2.3 1 1.3 EMERALD SHINER 1 2.8 2.3 2 2.5 SPOTFIN SHINER 9 25 4 9.3 13 16.5 SMALLMOUTH BUFFALO 2 5.6 3 7 5 6.3 GOLDEN REDHORSE 2 5.6 2 4.7 4 5.1 SHORTHEAD REDHORSE 2 5.6 2 2.5 CHANNEL CATFISH 1 2.8 1 2.3 2 2.5 GREEN SUNFISH 1 2.8 1 1.3 BLUEGILL 2 5.6 2 2.5 SMALLMOUTH BASS 3 8.3 3 3.8 LARGEMOUTH BASS 1 2.8 1 2.3 .'2 2.5 FRESHWATER DRUM 1 2.8 2 4.7 3 3.8 TOTAL FISH 36 100 43 100 79 100 TOTAL SPECIES 13 9 14 CPE (FISH/HOUR) 74 86 80 SEINING 1 2 5 Locations Combined SPECIES No. %No. %No. %No.%

GIZZARD SHAD 9 1 9 0.7 CENTRAL STONEROLLER 2 0.2 2 0.2 SILVER JAW MINNOW 4 0.4 4 0.3 EMERALD SHINER 157 64.1 36 55.4 817 90.2 1,010 83.1 BIGMOUTH SHINER 2 0.8 2 0.2

Table 4. Continued SPOTTAIL SHINER 1 0.1 1 0.1 ROSYFACE SHINER 2 0.8 2 0.2 SPOTFIN SHINER 67 27.3 27 41.5 21 2.3 115 9.5 SAND SHINER 7 2.9 14 1.5 21 1.7 Notropis sp. 3 1.2 3 0.2 SUCKERMOUTH MINNOW 2 0.2 2 0.2 BLUNTNOSE MINNOW 2 3.1 25 2.8 27 2.2 BULLHEAD MINNOW 2 0.2 9 0.7 7 2.9 I--

BROOK SILVERSIDE 8 0.9 8 0.7 SMALLMOUTH BASS 1 0.1 1 0.1 TOTAL FISH 245 100 65 100 906 100 100 1,216 TOTAL SPECIES 6 3 12 14

Table 5. Summary of DELT (Deformity, Erosion, Lesion, and Tumor) Anomalies on Fish Captured by Electrofishing and Seining near LaSalle Station, August 2013.

LOCATION LOCATIONS COMBINED 1 2 5 DELT DELT DELT DELIT EXAM DELT SPECIES GIZZARD SHAD -- 47 CENTRAL STONEROLLER -- 3 COMMON CARP SILVER JAW MINNOW -- 4 EMERALD SHINER -- 1012 BIGMOUTH SHINER -- 2 SPOTTAIL SHINER -- 1 ROSYFACE SHINER -- 2 SPOTFIN SHINER -- 128 SAND SHINER -- 21 Notropis sp. -- 3 SUCKERMOUTH MINNOW -- 2 BLUNTNOSE MINNOW -- 27 BULLHEAD MINNOW

--- 9 SMALLMOUTH BUFFALO GOLDEN REDHORSE -- 5 SHORTHEAD REDHORSE -- 2 CHANNEL CATFISH -- 2 BROOK SILVERSIDE -- 8 GREEN SUNFISH -- 1 BLUEGILL -- 2 SMALLMOUTH BASS -- 4 LARGEMOUTH BASS 2

FRESHWATER DRUM 1 2 3 66.7 TOTAL FISH 1 1 2 1295 0.2 PERCENT DELT ANOMALIES 0.1 0.1 0 0.2

Table 6. Mean Relative Weights of Fish Collected near LaSalle Station, August 2013.

LOCATION 1 2 5 LOCATIONS COMBINED SPECIES N MEAN MINIMUM MAXIMUM N MEAN MINIMUM MAXIMUM N MEAN MINIMUJM MAXIMUM N MEAN MINIMUM MAXIMUM GIZZARD SHAD 9 89 78 101 24 92 73 105 - - -- - 33 91 73 105 COMMON CARP -- 91 91 - -- - -- 1 91 91 91 1 91 SMALLMOUTH BUFFALO 2 76 73 80 3 80 74 84 - -- - -- 5 78 73 84 SHORTHEAD REDHORSE 2 73 70 76 -- 2 73 70 . 76 CHANNEL CATFISH 1 102 102 102 1 80 80 80 - -- -

- 2 91 80 102 BLUEGILL 2 114 110 118 -- 2 114 110 118 SMALLMOUTH BASS 2 93 85 100 1 91 91 91 3 92 85 100 LARGEMOUTH BASS 1 88 88 88 1 90 90 90 - -- - 2 89 88 90 FRESHWATER DRUM 1 84 84 84 2 91 3 88 84 93 88 93 - --

SPECIES COMBINED 20 89 70 118 32 90 73 105 1 91 91 91 53 90 70 118

Table 7. Benthic Macroinvertebrate Taxa Collected from the Illinois River in the Vicinity of LaSalle County Station, August 2013.

TURBELLARIA (flatworms) Diptera (true flies)

ENTOPROCTA Ceratopogonidae Urnatella gracilis Chaoborus ECTOPROCTA (bryozoans) Chironomidae (midges)

Plumatella Proclad ius ANNELIDA Ablabesmyia janta Oligochaeta (aquatic worms) Ablabesmyia mallochi Tubificidae La bru ndin ia Hirudinea (leeches) Nanocladius distinctus Placobdella Axarus CRUSTACEA Chi ronom us Amphipoda (sideswimmers) Cryptochironomus Hyalella azteca Dicrotendipes lucifer Gammarus Dicrotendipes modestus Apocorophium lacustre Dicrotendipes neomodestus ARACHNOIDEA (watermites) Dicrotendipes simpsoni Hydracarina Glyptotendipes INSECTA Paracladopel ma Ephemeroptera (mayflies) Polypedilum flavum Procloeon Polypedilum halterale grp.

Maccaffertium integrum Polypedilum scalaenum grp.

Maccaffertium terminatum Pseudoch ironom us Stenonema femoratum Rheotanytarsus Stenacron Tanytarsus Tricorythodes Xenochironomus Caenis MOLLUSCA Hexagenia Gastropoda (snails)

Odonata (damselflies and dragonflies) Ferrissia Argia Elimia Styl urus Bivalvia (mussels & clams)

Trichoptera (caddisflies) Corbicula fluminea Cheumatopsyche Sphaerium Hydropsyche orris Unionidae Potamyia flava Amblema plicata Hydroptila Quadrula pustulosa Oecetis Obliquaria reflexa Cyrnellus fraternus Leptodea fragilis Coleoptera (beetles) Dreissena polymorpha Dubiraphia Macronychus glabratus Stenelmis

Table 8. Density (#/m 2) and Relative Abundance (%) for Benthic Macroinvertebrate Taxa Collected in Hester-Dendy Samples Near LaSalle Station, August 2013.

LOCATION 2 LOCATION 3 LOCATION 4 TAXA #/m 2 %___ #/m2 %___

Turbellaria 293.8 1'c9.01 242.7 4.63 6.4 0.15 (a) 2.1 0.04 2.1 0.05 Plumatella Tubificidae 51.1 3 Ga mma rus -- 2.1 0.04 2.1 0.05 Apocorophium lacustre 17.0 1L.IO --

Stenacron -- 295.9 5.64 366.2 8.56 Maccaffertium integrum 106.5 65.89 159.7 3.04 379.0 8.86 Stenonema femoratum 2.1 C1.14 -- 6.4 0.15 Maccaffertium terminatum 19.2 1L.24 4.3 0.08 4.3 0.10 Tricorythodes -- 57.5 1.10 21.3 0.50 Argia 23.4 1/.52 42.6 0.81 23.4 0.55 Cyrnellus fraternus 534.4 3z4.57 519.5 9.90 276.8 6.47 Cheumatopsyche -- 161.8 3.08 1,043.3 24.38 Hydropsyche orris -- 2.1 0.04 215.0 5.02 Potamyia flava -- 2.1 0.04 121.4 2.84 Hydroptila 125.6 3.13 176.7 3.37 125.6 2.94 Macronychus glabratus -- 2.1 0.04 8

Stenelmis -- 12.8 0.24 Ceratopogonidae 17.0 /.10 68.1 1.30 51.1 1.19 Ablabesmyia janta 34.1 0.80 Ablabesmyia mallochi 17.0 1.10 51.1 1.19 Labrundinia 34.1 0.80 Nanocladius distinctus 23.4 1.52 238.5 4.55 51.1 1.19 Dicrotendipes modestus 140.5 9.09 919.8 17.53 374.7 8.76 Dicrotendipes neomodestus 38.3 2.48 919.8 17.53 357.7 8.36 Dicrotendipes simpsoni 78.8 5.10 783.5 14.94 323.6 7.56 Glyptotendipes 38.3 2.48 511.0 9.74 272.5 6.37 Polypedilum flavum 68.1 1.30 51.1 1.19 Rheotanytarsus 85.2 1.99 Ferrissia 17.0 1.10 53.2 1.01 Dreissena polymorpha 2.1 0.14 TOTAL BENTHOS (#/m 2) 1,545.7 1O00 5,246.2 100 4,279.5 100 TOTAL TAXA RICHNESS 18 23 25 EPT TAXA RICHNESS 5 9 10 aTaxon absent.

Table 9. Density (#/m2) and relative abundance (%) for benthic macroinvertebrate taxa collected in Ponar samples near LaSalle Station - August 2013.

LOCATION 1 LOCATION 2 LOCATION 3 LOCATION 4 TAXA Urnatella gracilis 4.8 0.79 Tubificidae 296.6 48.82 196.1 32.03 301.4 11.56 583.6 58.37 (a)

Placobdella AO0

'H-tl -0 Hyalella azteca 4.8 0.79 .. ..- 4.8 0.18 4.8 0.48 Gammarus .. ..- 33.5 1.28 23.9 2.39 Apocorophium lacustre 4.8 0.79 20)0.9 32.81 1832.2 70.28 9.6 0.96 Hydracarina ,1 fl 7SR ....

Procloeon 4.8 0.79 Stenacron 4.8 0.18 Caenis 9.6 1.57 9.6 1.56 43.1 1.65 4.8 0.48 Hexagenia 4.8 0.79 4.8 0.78 4.8 0.48 Stylurus 9.6 0.96 Cyrnellus fraternus ....- 43.1 1.65 4.8 0.48 Hydroptila ....- 9.6 0.37 Oecetis 9.6 1.57 4.8 0.78 19.1 0.73 Du bi raph ia 4.8 0.79 Sten elImis ....- 14.4 0.55 Chaoborus 4.8 0.79 Proclad ius 4.8 0.79 1IAA

-. ."t'.'-t

")9

£-.*'t ....

Ablabesmyia mallochi 4.8 0.79 ....- 9.6 0.37 4.8 0.48 Axarus ....- 9.6 0.37 Chi ronom us 31.3 13.28 23.9 0.92 Cryptochironomus 67.0 11.02 ....- 62.2 2.39 52.6 5.26 Dicrotendipes neomodestus 9.6 1.56 28.7 1.10 4.8 0.48 Dicrotendipes lucifer ....- 4.8 0.18 Dicrotendipes simpsoni 9.6 1.57 Glyptotendipes ....- 4.8 0.18 Pa raclad opel ma ....- 4.8 0.18 4.8 0.48 Polypedilum halterale grp. 71.8 11.81 119.1 3.12 19.1 0.73 100.5 10.05 Polypedilum scalaenum grp. 14.4 2.36 114.4 2.34 9.6 0.37 28.7 2.87 Pse udoch ironom us ....- 14.4 0.55 Tanytarsus 4.8 0.78 ....-

Xenochironomus ....- 4.8 0.18 Elimia 28.7 4.72 9.6 1.56 23.9 0.92 14.4 1.44 Corbicula fluminea 33.5 5.51 228.7 4.69 57.4 2.20 143.5 14.35 Sphaen u m 4.8 0.78 ....-

• Unionidae ....- 4.8 0.18 Amblema plicata ....- 4.8 0.18 Quadrula pustulosa ....- 4.8 0.18 Obliquaria reflexa ....- 4.8 0.18

Table 9 (cont.)

LOCATION 1 LOCATION 2 LOCATION 3 LOCATION 4 TAXA Leptodea fragilis 4.8 0.79 Dreissena polymorpha ....- 4.8 0.18 TOTAL BENTHOS (#/m 2 ) 607.5 100 612.3 100 2607.2 100 999.8 100 TOTAL TAXA RICHNESS 20 16 29 17 EPT TAXA RICHNESS 4 3 5 3 aTaxon absent.

Table 10. Density (#/m 2) and relative abundance (%) for benthic macroinvertebrate taxa collected in Ponar samples near LaSalle Station - August 1999. (EA 2000)

LOCATION 1 LOCATION 2 LOCATION 4 TAXA #/m 2 __ _

Aulodrilus limnobius -- 9.6 0.46 9.6 0.53 Aulodrilus pigueti 9.6 1.37 19.1 0.91 19.1 1.05 Pristina/Pristinella 9.6 0.53 Stephensoniana trivandrana -- -- 9.6 0.46 Branchiura sowerbyi .. ..- 162.6 7.76 47.8 2.63 Ilyodrilus ternpletoni 19.1 1.05 Limnodrilus cervix 96 1.37 220.1 10.50 95.7 5.26 Limnodrilus hoffmeisteri 7'6.5 10.96 229.6 10.96 181.8 10.00 Limnodrilus profundicola 2!8.7 4.11 38.3 1.83 Limnodrilus udekemianus 96 1.37 28.7 1.37 19.1. 1.05 Imm. tub. w/bifid chaetae 62.6 23.29 1100.3 52.51 1004.6 55.26 Imm. tub. w/hair & pectinate chaetae ....- 28.7 1.37 67.0 3.68 Centroptilum 19.1 1.05 Hexagenia limbata ....- 9.6 0.46 Stylurus notatus ....- 9.6 0.46 Du bira phia q 1 *7 --

Chironomidae 9.6 1.37 Cryptochironomus 191.4 27.40 47.8 2.28 124.4 6.84 Dicrotendipes neomodestus -- 9.6 0.53 Paratendipes basidens 9.6 1.37 Polypedilum halterale grp. 133.9 19.18 181.8 8.68 191.4 10.5 Corbicula fluminea 47.8 6.85 TOTAL BENTHOS (#/m2 ) 698.4 100 1817.8 100 100 2095.3 TOTAL TAXA RICHNESS 10 12 12 EPT TAXA RICHNESS 0 1 1

-* = Taxon absent

Table 11. Pre-operational HD mean density (#/m 2) and total taxa richness for Locations 1 - 4 on the Illinois River, 1974 - 1978. (Bio-Test 1975; Nalco 1976,1977, 1978; and Hazelton 1979)

Locations Sample Year/Event 1 2 3 4 1974 February Mean Density 30 15 25 --

Total Taxa Richness 5 3 4 --

May Mean Density 1,355 1,320 2,330 --

Total Taxa Richness 15 12 18 --

August Mean Density 4,330 3,435 5,800 5,285 Total Taxa Richness 23 25 23 25 November Mean Density 2,620 2,760 2,770 2,130

• Total Taxa Richness 20 16 18 16 1975 February Mean Density -- 65 305 80 Total Taxa Richness -- 6 13 10 May Mean Density 1,250 985 1,235 1,040 Total Taxa Richness 16 17 21 11 August Mean Density 2,635 2,745 4,575 2,175 Total Taxa Richness 34 30 29 29 November Mean Density 2,445 3,570 2,940 1,820 Total Taxa Richness 19 21 22 22 1976 February Mean Density 195 150 330 120 Total Taxa Richness 12 13 15 9 May Mean Density 945 975 1,105 1,100 Total Taxa Richness 11 9 15 10 August

.Mean Density 1,755 1,655 1,595 1,285 Total Taxa Richness 22 26 28 25 November Mean Density 1,465 3,650 3,585 2,920 Total Taxa Richness 19 19 19 13

Table 11 (cont.)

Locations Sample Year/Event 1 2 34 1977 February Mean Density 85 70 -- 80 Total Taxa Richness 4 4 -- 4 May Mean Density 10,500 10,785 9,385 8,150 Total Taxa Richness '20 21 23 20 August Mean Density 8,325 8,070 13,410 6,360 Total Taxa Richness 17 16 15 13 November Mean Density *1,620 860 2,295 800.

Total Taxa Richness 19 15 21 12 1978 February Mean Density 180 100 -- 115 Total Taxa Richness 3 6 -- 7_

May Mean Density 3,680 3,335 1,370 635 Total Taxa Richness 15 15 14 10 August Mean Density 3,510 3,893 2,210 3,535 Total Taxa Richness 30 30 29 27 November Mean Density 3,515 3,655 1,820 2,195 Total Taxa Richness 22 27 27 17

Table 12. Pre-operational Ponar and dip net mean density (#/m2) and total taxa richness for Locations 1 - 4 on the Illinois River and Locations 5 and 6 on South Kickapoo Creek, 1974 - 1978. (Bio-Test 1975; Nalco 1976,1977, 1978; and Hazelton 1979)

Locations Sample Year/Event 123 4 56 1974 February Mean Density 1,215 1,999 3,270 1,768 2,683 Total Taxa Richness 10 22 32 32 33 May Mean Density 62 225 91 80 747 454 Total Taxa Richness 7 6 16 22 16 6 August Mean Density 274 359 629 610 662 467 Total Taxa Richness 9 10 8 15 15 16 November Mean Density 658 148 482 1,097 110 2,345 Total Taxa Richness 9 5 8 21 10 14 1975 February Mean Density 394 254 1,277 53 1,019 38 Total Taxa Richness 12 22 21 8 42 1 May Mean Density 284 346 978 1,875 1,338 285 Total Thxa Richness 6 9 18 28 17 17 August Mean Density 119 297 492 879 374 1,253 Total Taxa Richness 7 7 16 26 16 27 November Mean Density 326 203 846 1,658 345 4,791 Total Taxa Richness 13 7 12 15 16 37 1976 February Mean Density 52 1,040 1,696 2,*325 520 113 Total Taxa Richness 2 26 20 31 27 12 May Mean Density 293 468 90 435 411 317 Total Taxa Richness 7 12 15 13 12 10 August Mean Density 326 246 402 331 198 378 Total Taxa Richness 9 13 12 10 12 6 November Mean Density 246 274 99 227 156 439 Total Taxa Richness 7 10 4 12 9 8

Table 12 (cant.)

Locations Sample Year/Event 1 2 3 4 5 6 1977 February Mean Density 3,638 4,470 4,848 3,482 1,871 Total Taxa Richness 14 19 14 23 23 May Mean Density 5,972 9,053 4,716 12,970 2,561 1,545 Total Taxa Richness 9 12 12 13 14 9 August Mean Density 3,563 5,330 4,871 5,741 5,273 2,736 Total Taxa Richness 10 10 10 13 18 24 November Mean Density 822 1,394 1,602 1,243 723 222 Total Taxa Richness 7 10 10 15 13 8 1978 February Mean Density 888 2,424 6,265 1,616 477 Total Taxa Richness 20 21 32 36 26 May Mean Density 387 250 189 430 170 576 Total Taxa Richness 8 7 10 21 13 12 August Mean Density 354 321 477 425 893 Total Taxa Richness 12 13 14 20 16 November Mean Density 501 444 869 1,858 430 1,328 Total Taxa Richness 14 11 13 19 11 15

APPENDIX A LaSalle County Station Fish Data August 2013

APPENDIX A - LaSa lie Station Fish Data SITE: ILLINIOS RIVER GEAR: ELECTRO LOCATION: 1 SAMPLING DISTANCE (in): 500 SAMPLING DURATION (min): 29 START DATETIME: 13AUG13:12:32 END DATETIME: 13AUG13:13:01 LENGTH WEIGHT PLUS BATCH SPECIES Wr (ims) (g) COUNT WEIGHT ANOMALY 1 ANOMALY 2 ANOMALY 3 GIZZARD SHAD 78 255 .140 GIZZARD SHAD 88 228 110 GIZZARD SHAD 95 240 140 GIZZARD SHAD 87 225 105 GIZZARD SHAD 85 259 160 GIZZARD SHAD 89 260 170 GIZZARD SHAD 101 215 105 GIZZARD SHAD 94 229 120 GIZZARD SHAD 85 248 140 GIZZARD SHAD 135 29 CENTRAL STONEROLLER1 2 EMERALD SHINER 1 2 SPOTFIN SHINER 9 17 SMALLMOUTH BUFFALO 80 370 700 *Missing Scales Regen. Scales SMALLHOUTH BUFFALO 73 379 685 *Regen. Scales GOLDEN REDHORSE 365 440 GOLDEN REDHORSE. 271 210 SHORTHEAD HEDHORSE 76 355* 390 *Regen. Scales SHORTHEAD REDHORSE 70 385 460 CHANNEL CATFISH 102 500 1250 GREEN SUNFISH- 55 4 BLUEGILL 110 90 14 BLUEGILL 118 88 14 SHALLHOUTH BASS 85 195 85 SMALLMOUTH BASS 100 242 200 SNALLMOUTH BASS 145 39 LARGEHOUTH BASS 88 288 300 FRESHWATER DRUM 84 475 1200 *Moderate Erosion Regen. Scales Other SITE: ILLINIOS RIVER GEAR: ELECTRO LOCATION: 2 SAMPLING DISTANCE (m): 500 SAMPLING DURATION (mink: 30 START DATETINE: 13AUG13:10:40 END DATETINE: 13AUG13:11:10 LENGTH WEIGHT PLUS BATCH SPECIES Wr (nsm) (g) COUNT WEIGHT ANOMALY 1 ANOMALY 2 ANOMALY 3 GIZZARD SHAD 73 345 340 GIZZARD SHAD 82 192 60 GIZZARD SHAD 105 195 80 GIZZARD SHAD 103 210 100 GIZZARD SHAD 79 209 75 GIZZARD SHAD 100 205 90 GIZZARD SHAD 103 196 80 GIZZARD SHAD 101 215 105 GIZZARD SHAD 175 66 GIZZARD SHAD 96 202 82 GIZZARD SHAD 93 198 75 GIZZARD SHAD 101 180 60 GIZZARD SHAD 97 227 120 GIZZARD SHAD 96 208 90 GIZZARD SHAD 90 211 88 GIZZARD SHAD 94 202 80 GIZZARD SHAD 93 214 95 GIZZARD SHAD 84 200 70 GIZZARD SHAD 100 204 88 GIZZARD SHAD 78 264 155 GIZZARD SHAD 76 284 192 GIZZARD SHAD 82 285 210 GIZZARD SHAD 98 250 165 GIZZARD SHAD 98 212 98 GIZZARD SHAD 77 215 80 GIZZARD SHAD 3 320 COMMON CARP 91 550 2100 EMERALD SHINER 1 SPOTFIN SHINER 4 SMALLMOUTH BUFFALO 430 1050 Regen. Scales SMALLMOUTH BUFFALO 81 425 1100 SMALLMOUTH BUFFALO 84 356 650 Regen. Scales GOLDEN REDHORSE 390 480 GOLDEN REDHORSE 200 91 CHANNEL CATFISH 80 365 350 LARGEMOUTH BASS 90 274 260 FRESHWATER DRUM 88 512 1610 Fungus Slight Erosion Other FRESHWATER DRUM 93 435 1010 A-i

APPENDIX A (cont.)

SITE: ILLINIOS RIVER GEAR: SEINE LOCATION: 1 SAMPLING DISTANCE (m):

SAMPLING DURATION (rai)  : ii START D)ATETIME: 12AUG13:18:53 END DATETIME: 12AUG13:19:04 LENGTH WEIGHT PLUS BATCH SPECIES Wr (mmn) (g) COUNT WEIGHT ANOMALY 1 ANOMALY 2 ANOMALY 3 EMERALD SHINER *

• 112 155 EMERALD SHINER * . 22 5 EMERALD) SHINER * . . 23 2-BIGZ4OUTH SHINER * *

• 2 1-ROSYFACE SHINER * *

• 2 1 SPOTFIN SHINER * *

• 6 6 SPOTFIN* SHINER 3 1 SPOTFIN SHINER 58 3 SAND SHINER 5 2 SAND SHINER 2 Natropis sp. 3 61 BULLHEAD MINNOW

• 6 111 BULLHEAD MINNOW * *

• 1 1 SITE: ILLINIOS RIVER GEAR: SEINE LOCATION: 2 SAMPLING DISTANCE (in): SAMPLING DURATION (min): 36 START D*ATETIME: 12AUG13:18:05 END DATETIME: 12AUG13:18:41 LENGTH WEIGHT PLUS BATCH SPECIES Wr (nf) (g) COUNT WEIGHT ANOMALY 1 ANOMALY 2 ANOMALY 3 EMERALD SHINER * *

• 34 33 EMERALD SHINER * *

• 2 1 SPOTFIN SHINER * . 24 35 SPOTFIN SHINER - .

• 3 1 BLUNTNOSE MINNOW 2 4 SITE: ILLINIOS RIVER GEAR: SEINE LOCATION: 5 SAMPLING DISTANCE (mn):

• SAMPLING DURATION (min): 15 START DATETIME: 12AUG13:17:55 END DATETIME: 12AUG13:1B:10 LENGTH WEIGHT PLUS BATCH SPECIES~ Wr (mm) (g) COUNT WEIGHT ANOMALY 1 ANOMALY 2 ANOMALY 3 GIZZARD SHAD 113 15 GIZZARD SHAD 86 7 GIZZARD SHAD 97 11 GIZZARD SHAD 95 10 GI ZZARD SHAD 106 14 GI ZZARD SHAD 102 12 GIZZARD SHAD 90 9 GIEZARD SHAD GIZZARD SHAD

• 96 10 CENTRAL STONEROLLER 4-S ILVERJAW MINNOW 5 EMERALD SHINER 3 EMERALD SHINER * * - i00 206 EMERALD SHINER * *

• 401 826 EMERALD SHINER * *

• 313 644 EMERALD SHINER 1 SPOTTAIL SHINER 2 SPOTFIN SHINER 11 15 SPOTFIN. SHINER * *

• 5 2 SPOTFIN SHINER * *

• 5 1 SAND SHINER 13 SAND SHINER 1 SUCEERMOUTH MINNOW 3 BLUNTNOSE MINNOW * *

• 25 34 BULLHEAD MINNOW 1 BROOK SILVERSIDE 6 SMALLMOUTH BASS 91 187 79 A-2

APPENDIX B LaSalle County Station Benthic Macroinvertebrate Data August 2013

APPENDIX B - LaSalle Station Benthic Macroinvertebrate Study - Hester-Dendy Data GEAR= HESTER, DATE= AUG 2013, and LOCATION= LOC 2 U/S TmX *# __#/zn2_ %__

Turbellaria 138- 293.8 19.01 Tubificinae 24 51.1 3.31 Apocorophium lacustre 8 17.0 1.1I0 Maccaffer tium integrum 50 106.5 6.89 Stenonema femoratum 1 2.1 0.14 Maccaffertium terminatum 9 19.2 1.24 Argia 1i 23.4 1.52 Cyrnellus fraternus 251 534.4 34.57 Hydroptila 59 125.6 8.13 Ceratopogonidae 8 17.0 1.1i0 Ablabesmyia mallachi 8 17.0 1.1i0 Nanocladius distinctus 11 23.4 1.52 Dicrotendipes modestus 66 140.5 9.09 Dicrotendipes neomodestus 18 38.3 2.48 Dicrotendipes simpsoni 37 78.8 5.1i0 Glyptotendipes 18 38.3 2.48 Ferrissia 8 17.0 1.1I0 Dreissena polymorpha 1i 2.1 0.14 TOTAL BENTHOS 726 1,545.7 100.00 GEAR= HESTER, DATE= AUG 2013, and LOCATION= LOC 3 D/S TmX #__* __#/zn2_ %__

Turbellaria 114 242.7 4.63 Plumatella 1 2.1 0.04" Gammarus 1 2.1 0.04 Stenacron 139 295.9 5.64 Maccaffertium integrum 75 159.7 3.04 Maccaffertiuma terminatum 2 4.3 0.08 Tricorythodes 27 57.5 1.1I0 Argia 20 42.6 0.81 Cyrnellus fraternus 244 519.5 9.90 Cheumatopsyche 76 161.8 3.08 Hydropsyche orris 1 2.1 0.04 Potamyia flava 1 2.1 0.04 Hydroptila 83 176.7 3.37 Macronychus glabratus 1 2.1 0.04 Stenelmis 6 12 :8 0.-24 Ceratopogonidae 32 68.1 1.30 Nanocladius distinctus 112 238.5 4.55 Dicrotendipes modestus 432 ' 919.8 17.53 Dicrotendipes neomodestus 432 919.8 17.53 Dicrotendipes sizspsoni 368 783.5 14.94 Glyptotendipes 240 511.0 9.74 Polypedilum flavum 32 68.1 1.30 Ferrissia 25 53.2 1.01 TOTAL BENTHOS 2,464 5,246.2 100.00 GEAR= HESTER, DATE= AUG 2013, and LOCATION= LOC 4 D/S TmX __#_ __#/ni2_ %__

Turbellaria 3 6.4 0.15 Plumatella 1 2.1 0.05 Gairrnarus 1 2.1 0.05 Stenacron 172 366.2 8.56 Maccaffertium integrum 178 379.0 8.86 Stenonema femoratum 3 6.4 0.15 Maccaffertium terminatum 2 4.3 0.1I0 Tricorythodes 10 21.3 0.50 Argia 11 23.4 0.55 Cyrnellus fraternus 130 276.8 6.47 Cheumatopsyche 490 1,043.3 24.38 Hydropsyche orris 101 215.0 5.02 Potamyia flaya 57 121.4 2.84 Hydroptila 59 125.6 2.94 Ceratopogonidae 24 51.1 1.19 Ablabesmyia janta 16 34.1 0.80 Ablsbesmyia msallochi 24 51.1 1.19 Labrundinia 16 34.1 0.80 Nanocladius distinctus 24 51.1 1.19 Dicrotendipes modestus 176 374.7 8.76 Dicrotendipes neomodestus 168 357.7 8.36 B-i

APPENDIX B (cont.)

Dicrotendipes sjimpsoni 152 323.6 7.56 Glyptotendipes 128 272.5 6.37 Palypedilum flavum 24 51.1 1.19 Rheotanytarsus 40 85.2 1.99 TOTAL BENTHOS 2,010 4,279.5 100.00 LASALLE STATION BENTHIC X4ACROINVERTEBRATE STUDY - PONAR DATA GEAR= PONAR, DATE= AUG 2013, and LOCATION= LOC 1 TAm 0 #__/sQ ___

Urnatella gracilis 1 4.8 0.79 Tubificinae 62 296.6 48.82 Hyalella azteca 1 4.8 0.79 Apocorophium lacustre 1 4.8 0.79 Procloeon 1 4.8 0.79 Caenis 2 9.6 1.57 Hexagenia 1 4.8 0.79 Oecetis 2 9.6 1.57 Dubiraphia 1 4.8 0.79 Chaoborus 1 4.8 0..79 Procladius 1 4.8 0.79 Ablabesmyia mallochi 1 4.8 0.79 Cryptochironomus 14 67.0 11.02 Dicrotendipes sisipsoni 2 9.6 1.57 Paracladopelma 4 19.1 3.15 Polypedilum halterale grp. 15 71.8 11.81 Polypedilum scalaenum grp. 3 14.4 2.36 Elimnia 6 28.7 4.72 Corbicula fluminea 7 33.5 5.51 Leptodea fragilis 1 4.8 0.79 TOTAL BENTHOS 127 607.5 100.00 GEAR= PONAR, DATE= AUG 2013, and LOCATION= LOC 2 TmX 0 #__/m2_ %__

Tubificinae 41 196.1 32.03 Placobdella 1 4.8 0.78 Apocorophium lacustre 42 200.9 32.81 Hydracarina 1 4.8 0.78 Caenis 2 9.6 1.56 Hexagenia 1 4.8 0.78 Oecetis 1 4.8 0.78

.Procladius 3 14.4 2.34 Chironomus 17 81.3 13.28 Dicrotendipes neomodestus 2 9.6 1.56 Polypedilumi halterale grp. 4 19.1 3.12 Polypedilumi scalaenum grp. 3 14.4 2.34 Tanytarsus 1 4.8 0.78 Elimia 2 9.6 1.56 Corbicula flumiinea 6 28.7 4.69 Sphaeriuxn 1 4.8 0.78 TOTAL BENTHOS 128 612.3 100.00 GEAR= PONAR, DATE= AUG 2013, and LOCATION= LOC 3 TmX #/m2 %__

Tubificinae 63 301.4 11.56 Hyalella azteca 1 4.8 0.18 Gasmarus 7 33.5 1.28 Apocorophiumi lacustre 383 1,832.2 70.28 Stenacron 1 4.8 0.18 Caenis 9 43.1 1.65 Cyrnellus fraternus 9 43.1 1.65 Hydroptila 2 9.6 0.37 Oecetis 4 19.1 0.73 Stenelmis 3 14.4 0.55 Ablabesmyia mallochi 2 9.6 0.37 Axarus 2 9.6 0.37 Chironomus 5 23.9 0 .92 Cryptochironomus 13 62.2 2.39 Dicrotendipes neomodestus 6 28.7 1.10 Dicrotendipes lucifer 1 4.8 0.18 Glyptotendipes 1 4.8 0.18 B-2

APPENDIX B (cont.)

Paracladopelma 1 4.8 0.18 Polypedilum halterale grp. 4 19.1 0.73 Polypedilum scalaenum grp. 2 9.6 0.37 Pseudochironomus 3 14.4 0.55 Xenochironomus 1 4.8 0.18 Elimia 5 23.9 0.92 Corbicula fluminea 12 57.4 2.20 Unionidae 1 4.8 0.18 Amblema plicata 1 4.8 0.18 Quadrula pustulosa 1 4.8 0.18 Obliquaria reflexa 1 4.8 0.18 Dreissena polymorpha 1 4.8 0.18 TOTAL BENTHOS 545 2,607.2 100.00 LASALLE STATION BENTHIC NACROINVERTEBRATE STUDY - PONAR DATA GEAR= PONAR, DATE= AUG 2013, and LOCATION= LOC 4 TAX *_ __#1ms2_ _

TmX Tubificinae 122 583.6 58.37 Nyalella azteca 1 4.8 0.48 Gamsmarus 5 23.9 2.39 Apocorophium lacustre 2 9.6 0.96 Caenis 1 4.8 0.48 Hexagenia 1 4.8 0.48 Stylurus 2 "9.6 0.96 Cyrnellus fraternus 1 4.8 0.48 Ablabesmyia msallochi 1 4.8 0.48 Cryptochironomus 11 52.6 5.26 Dicrotendipes neomodestus 1 4.8 0.48 Paracladopelmta 1 4.8 0.48 Polypedilumn halterale grp. 21 100.5 10.05 Polypedilum scalaenum grp. 6 28.7 2.87 Elimia 3 14.4 1.44 Corbicula fluminea 30 143.5 14.35 TOTAL BENTHOS 209 999.8 100.00 B-3