ML15273A422
| ML15273A422 | |
| Person / Time | |
|---|---|
| Site: | LaSalle  |
| Issue date: | 02/28/2011 |
| From: | HDR Engineering |
| To: | Exelon Nuclear, Office of Nuclear Reactor Regulation |
| Shared Package | |
| ML15273A423 | List: |
| References | |
| RIS-15-254 | |
| Download: ML15273A422 (19) | |
Text
ER REFERENCES TO BE DOCKETED ATTACHMENT 8
WC-MW-1 14 Revision 0 52~ Page 5 of 6 ATTACHMENT 1 Predefine Data Package Cover Sheet Page 1 of I Procedure#
Title _7.O.*r. L~-r.s-MO -\~' Ui: 00 P PM. ID: "1'r--,7*-O\
PREDEFINE INFORMATIDN:
Due Date: *I O il Work LateOrderDate:
Tech Spec: NO* YES SIGNATURE AUTHORIZATION/APPROVAl LS Opposite Train Operable YES NO NA Authlorization to Start Work N'4 A Work Started By*i*,
- Work Completed By ,*"
Surveillance Found Within Acetnc rtei NO Surveillance Left Within AcetneCritel a I(
Supv Review of Work Complete ig ature Time Date Update Predefine (circle one of the following):
S- COMPLETE/PORTIONS UNSAT -PARTIAL -FAILED COMMENTS RE VIEW OF RESULTS
________________________A~:, __.___..
Title Signature Date
II ZEBRA MUSSEL MONITORING PROGRAM At LaSalle Nuclear Station, 2010G I
Prepared for EXELON NUCLEAR 3:
i]i February 2'011 J* HIDR Engineering, Ine*
Environmental Science & Engineering. Consultants.
id.
10207 Lucas Road, Woodstock, lilinois 60098.
Ii "I
ZEBRA MUSSEL MONITORING PROGRAM At LaSalle Nuclear Station, 2010 Prepared for EXELON NUCLEAR Warrenville, Illinois HiDR Engineering, Inc.
Environmental Science & Engineering Consultants 10207 Lucas Road, Woodstock, nllinois 60098
.1
.I TABLE OF CONTENTS iiPage No.
TABLE OF CONTENTSi
- LIST OF TABLES ii 1LIST OF FIGURES iii
1.0 INTRODUCTION
1 2.0 METHODS 2 2.1 Artificial Substrate Sampling 2 2.2 Water Quality Measurements 4 J3.0 RESULTS AND DISCUSSION 5 3.1 Artificial Substrate Sampling 5 3.1.1 Intake Substrates 5 3.1.2 Cooling Lake Substrates 5
- 3.2.1 3.2 Water Quality Measurements Intake Water Quality 3.2.2 Cooling Lake Water Quality6 5
6 4.0
SUMMARY
AND RECOMMENDATIONS I11
]
5.0 REFERENCES
12 I
i
LIST OF TABLES Table No. Caption Page No.
3-1 Water Quality Parameters and Mean Zebra Mussel Densities Collected Monthly During the 2010 Sampling Eflort at LaSalle Intake.7 3-2 Water Quality Parameters and Mean Zebra Mussel Densities Collected Monthly During the 2010 Sampling Effort at LaSalle Cooling Lake. 8 ii
j LUST OF FIGURES
.Figure No. Caption Page No.
J 3-1 Temperature Profile at the LaSalle Station Intake for the Period of 7 April to 10 November, 2010.
9 3-2 Temperature Profile at LaSalle Lake for the Period of 7 April to l0 November, 2010. 10 ill
1.0 INTRODUCTION
Exelon Nuclear has sponsored a zebra mussel (Dreissena. polymorpha) monitoring program since 1990 (ZEI 2003, LMS 2004-2005, HDR1LMS 2006-2007, HDR Engineering, Inc. 2008-2010).
Stations electing to continue monitoring in 2010 include Braidwood Station, Byron Station, and LaSalle Station. Since the monitoring program began in 1990, zebra mussel colonization has occurred at all three of these stations, to varying degrees.
The principal objectives of these monitoring efforts at LaSaile are to:
- Detect the presence and densities of zebra mussels at the intake structure and source water bodies.
- Evaluate year-to-year changes in the zebra mussel populations, including settlement densities.
- Provide a basis for examining possible effects of zebra mussels on Station operations.
1 1HDR Engineering, Inc.
"] 2.0 METHODS 32.1 ARTIFICIAL SIYBSTRATE SAMPLING To determine zebra mussel settlement densities, artificial substrates were placed in front of the*
3LaSalle Station river intake in the Illinois River. Three arrays of artificial substrate samplers were placed, which included two Type A substrates and one Type B substrate.
Iiinside Type A substrate consisted of two pieces of PVC pipe that were 15 cm (6 in.) long and had an diameter of 5.08 cm (2 in.) and an outside diameter of 6.08 cm (2.39 in.). Each pipe was cut 3from in half lengthwise and rejoined using hose clamps. The PVC substrates were deployed by cable the railing above the intake in a central location. Concrete blocks were used to anchor each sampling array to the bottom of the intake. One cumulative substrate sampler and one monthly 3(periodic) amry was deployed at each sampling location. 'The PVC substrates were attached to the cable approximately 0.5 and 1.5 meters below the surface of the water, respectively. Artificial
~substrates were installed in firmt of the intake on 7 ApriL. Retrieval of Type A substrates occurred on a monthly basis and consisted of gently removing the series of PVC pipes from the water and
~placing each individual sample into a labeled container containing a solution of 400/0 isopropyl alcohol. New PVC substrates were then placed on the monthly array and returned to their original positions in the water column. Cumulative substrates remained in the water from 7 April I to 10 November.
]Type B substrate arrays consist of a microscope slide rack (Dura-Sampler) attached in the same manner as the PVC Type A arrays. Retrieval of Type B substrates occurred on a monthly basis
]adrequired gently removing temicroscope siesamplers frmtewater adplacing eight glass slides from each sampler into an appropriately labeled container containing 40% isopropyl
~alcohol. New labeled glass slides were then installed into the slide samplers and returned to their original location. PVC and glass were chosen as principle substrates because it has been shown
]that PVC is a preferred substrate (Walz 1975), while glass slides allow for a standard measurement of settlement densities and examination under a low powered binocular microscope I (Marsden 1992)."
~Two Type A and one Type B sample arrays were placed within the LaSalle Station Cooling Lake I on 7 April. Substrates were set at depths of 1.0, 3.0 and 5.0 meters on a cable suspended between 2HDR Engineering, Inc.
a surfa~ce float and a concrete anchor. Both cumulative and monthly substrates were set. Monthly retrieval of Type A and Type B substrates was conducted as described above. Sampling was concluded on 10 November with the removal of all the substrates fr'om the LaSalle Station intake and cooling lake. Monitoring was designed to provide information on accumulated infestation and growth of settled zebra mussels throughout the growing season.
Settlement was deternined by scraping the inside and outside of both halves of each PVC pipe sampler. The area of the substrate that was sampled was calculated by first finding the inside and outside cirumferences of the PVC pipe.
c=,rd Where:
o circumference of a circle
- r=3.l4
.d =diameter of a circle The total area of PVC pipe that was sampled was calculated by summing the total area sampled inside of the PVC pipe plus the total area sampled outside of the PVC pipe. The inside and outside area of the PVC pipe was calculated using the following equation:
A--cxl Where:
A =area sampled c = circumference of a,circle I = length of the PVC pipe The number of mussels found was then converted from number per square inch to the conventional number per square meter. Shell lengths (measured along the longest axis) were also measure for up to 50 individuals to obtain maximum, minimum, and mean sizes for each substrate. Shells less than 1.0 nun in length were measure to the nearest 25 microns using an ocular micrometer that was 3HDR Engineering, lne.
calibrated to a stage micrometer. Shells greater than 1.0 mmn in length were measured to the nearest half-millimeter using a standard metric ruler.
2.3 WATER QUALITY MEASUREMENTS Four physicochemical parameters (temperature, dissolved oxygen, pH and conductivity) were measured in conjunction with the sampling program. These data were collected at each location prior to each sampling effort. All physicochemical measurements were made at one meter below the surface of the water. Temperature (0C), dissolved oxygen (ppm), and conductivity (pruhos) were measured using an YSI Model 85 Oxygen, Conductivity, Temperature, and Salinity meter.
An Oakton WP pH Tester! was used to determine ptR Temperature monitors (Onset OpticStowAway Temp Loggers) were attached at mid-depth to the cumulative and monthly substrates to rec~ord hourly changes in temperature throughout the entire sampling period. Periodic temperature data was downloaded on a monthly basis, while cumulative temperature recordings were monitored by a separate logger from 7 April through 10 Novemnber.
4 41IDR Engineering, Inc.
3.0 RESULTS AND DISCUSSION 3.1 ARTIFICIAL SUBSTRATE SAMPLING 3.1.1 Intake Substrates No zebra mussels were collected on the monthly substrates from the LaSalle Station Cooling Lake throughout the seven month sampling period (Table 3-1). Analysis of the surface cumulative substrate (0.5m) showed heavy filamentous algae covered the outside of the substrate.
The inside of the pipe was primarily covered (>70%) with bryozoan colonies and caddisfly larvae. The bottom cumulative substrate (1.Sin) was covered both inside and outside with caddisfly larvae and other aquatic invertebrates.
3.1.2. Cooling Lake Substrates No zebra mussels were collected on the monthly substrates from the LaSalle Station Cooling Lake throughout the seven month sampling period (Table 3-2). Cumulative substrates were 90-100% covered with bryozoan colonies that measured 1/8 to 3/8 inch thick. All depths sampled (1.0, 3.0, 5.0 m) were observed to have similar colonization.
3.2 WATER QUALITY MEASUREMENTS Four physicochemical parameters (temperature, dissolved oxygen, pH and conductivity) were measured in conjunction with the sampling program. Water quality measurements were taken during each of the sampling dates at the LaSalle Station intake and cooling water lake (Table 3-1 and 3-2).
3.2.1 Intake Water Quality Temperatures at the LaSalle Station intake ranged from 10.28 C on 8 November to 31.88 C on 14 August (Figure 3-1). Dissolved oxygen (DO) concentrations ranged from 6.4 ppm on 4 August to 12.0 ppm on 5 May (Table 3-1). Recordings for p11 ranged from 7.4 to 8.3 and conductivity ranged from 525 to 842 jimhos throughout the sampling season.
5 5HDR Engineering, Inc.
I I
3.2.2 Cooling Lake Water Quality Temperatures from the LaSalle Station cooling lake ranged from 16.60 C on 8 November to 38.70.
C on 12 August (Figure 3-2). Dissolved oxygen (DO) concentrations ranged from 6.2 ppm on 9 June to 10.2 ppm on 7 April (Table 3-2). Recordings for pH ranged from 8.2 to 8.8 and conductivity ranged from 1037 to 1239 gmhos throughout the sampling season.
3 2
]
3
]
]
]
iJ 6HDR Engineering, Jnc.
Table 3-1. Water Quality Parameters and Mean Zebra Mussel Densities Collected Monthly During the 2010 Sampling Effort at LaSaile Intake.
Water Quality Juveniles Adults Days/ Temp. DO Cond. Sample Date Sample Time .("C) (ppm). pH (jimhos) Depth Glass PVC Glass PVC 4/07/10 - 06:45 17.1 9.3 7.9 842 - -
5/05/10 28 08:20 19.6 12.0 7.9 834 0 0 0 0 6/09/10 35 08:12 21.6 7.3 8.0 645 0 0 0 0
-4 -7/01/10 22 08:10 25.0 7.1 7.4 554 0 0 0 0 8/04/10 34 15:14 26.5 6.4 7.5 525 0 0 0 0 9/08/10 35 08:14 23.0 6.8 8.0 727 0 0 0 0 10/06/10 28 08:37 18.7 8.1 8.3 639 0 0 0 0 11/10/10 35 08:45 11.6 7.2 8.0 586 0 . 0 0 0
- ilI . . . . . . ..- -T . . .. -"--.. ,. * . . .
I I-ll. I . .. '
_____ -. -.-..- -~ **-~; L~: L~: L~.J. L~~i ~ ~A ~=.-A. ~
Table 3-2. Water Quality Parameters and Mean Zebra Mussel Densities Collected Monthly During the 2010 Sampling Effort at LaSalle Cooling Lake.
Water Quality Juveniles Adults Days/ Temp. DO Cond. Sample Date Sample Time (0 C) (ppm) .. pH (itmhos) Depth Glass PVC Glass PVC 4/07/10 - 07:45 21.5 10.2 8.2 1145----
5/05/10 28 09:22 23.6 10.0 8.3 1157 0 0 0 0 00 6/09/10 35 09:28 28.2 6.2 8.3 1239 0 0 0 0 7/01/10 22 09:00 32.0 7.9 8.6 1037 0 0 0 0 8/04/10 34 14:36 34.8 6.5 8.5 1072 0 0 0 0 9/08/10 35 08:52 23.6 6.8 8.6 1103 0 0 0 0 10/06/10 28 09:23 24.7 6.5 8.8 1125 0 0 0 0 11/10/10 35 09:28 19.9 6.8 8.4 1218 0 0 0 0
35 30 -
25
]5 di Figure 3-1. Temperature Profile at the LaSaile Station Intake for the Period of 7 April to 10 November, 2010.
45 40 35 30 15 01 0 i a Iii ia
,,,., o- --. - ,_ ,,..
- 0 Figure 3-2. Temperature Profile at LaSalle Lake for the Period of 7 April to 10 November, 2010.
4.0
SUMMARY
ANDU RECOMMENDATIONS In 2010, no zebra mussels were found on the monthly or cumulative intake substrates over the seven month sampling period. Periodic and cumulative substrate samples located in the LaSalle cooling lake also resulted in no zebra mussel settlement. The cumulative substrates in LaSalle Lake were 90-100% coverage by bryozoans colonization at all sample depths.
Early detection of zebra mussel colonization is essential for uninterrupted Station operation.
Because zebra mussels are present in the Illinois River, they are continually reintroduced to the cooling lake through make-up water. Continued monitoring is important to recognize the circumstances associated with zebra mussel infestation and colonization.
ll 11HDR Engineering, Inc.
5.0 REFERENCES
HDR Engineering, Inc. 2010. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2009. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.
HDR Engineering* Inc. 2009. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2008. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.
HDR Engineering, Inc. 2008. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2007. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.
HDR/LMS. 2007. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2006.
Prprdfor Exelon Nuclear. Warren'ville, IL. 12 pp.
HDRILMS. 2006. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2005.
Preprted for Exelon Nuclear. Warrenville, IL. 12 pp.
Lawler, Matusky & Skelly Engineers, LLP (LMS). 2005. Zebra Mussel Monitoring Program at LaSafle Nuclear Station, 2004. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.
Lawler, Matusky & Skelly Engineers, LLP (LMS). 2004. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2003. Prepared for Exelon Nuclear. Warrenviile, IL. 12.pp.
Marsden, J. E. 1992. Standard Protocols for Monitoring and Sampling Zebra Mussel Illinois Natural History Survey. Biological Notes 138, April 1992.
Walz,* V. N. 1975. The Settlement of Larvae of Dreissena po lymorpha on Artificial Substrates. Arch. Hydrobiol. Suppl. 47: 423-431.
Zambrana Engineering, Inc. 2003. Byron Station 2002 Zebra Mussel (Dreissena polymorpha)
Monitoring Program Annual Report.
12 12HDR Engineering, Inc.