Text

Discharge Monitoring Report for October 2018
	ML18318A194
Person / Time
Site:	Sequoyah
Issue date:	11/08/2018
From:	Anthony Williams; Tennessee Valley Authority
To:	; Document Control Desk, Office of Nuclear Reactor Regulation
References
	Download: ML18318A194 (99)
	v • d • e

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name _ _IY* ^ro^YA^r^C^E^R^^NT DISCHARGE MONITORING REPORT (DMR)

(SUBR01) OMB No. 2040-0004 M6ress_ _P. a.BOX_2poo_

TN0026450 101 G F - FINAL aNIEROFFICEOPS^NLSQN)

SODDY_-^AJSYiXN_3Z3§4 PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Facjijty__TVA-SEQUOYAH NUCLEAR PLANT EFFLUENT Juration. _HAMILTOJi_CO]unjY MONITORING PERIOD YEAR MO DAY To YEAR MO DAY

- - NO DISCHARGE [^J ***

ATTN:Millicent Garland From 18 10 01 18 10 31 NOTE: Read instructions before completing this form.

X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE OF TYPE EX ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ********

39.3 04 0 31/31 RCORDR MEASUREMENT CENTIGRADE PERMIT ******** ********

Req. Mon. DEG.C. CONTI CALCTD 00010 1 0 REQUIREMENT EFFLUENT GROSS DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ********

27.1 0 31/31 MODELD 04 MEASUREMENT CENTIGRADE 00010 Z 0 PERMIT ******** ******** **** ******** ********

30.5 DEG.C. CONTI CALCTD REQUIREMENT INSTREAM MONITORING DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ********

2.5 31/31 CALCTD 0

04 MEASUREMENT UPSTRM DEG.C 00016 1 S PERMIT ******** ******** **** ******** ********

3.0 DEG.C. CONTI CALCTD REQUIREMENT EFFLUENT GROSS DAILY MX NUOUS FLOW, IN CONDUIT OR THRU 31/31 RCORDR SAMPLE ******** ********

03 0

MEASUREMENT TREATMENT PLANT Req. Mon. CONTI RCORDR 50050 1 0 PERMIT ********

MGD ******** ********

REQUIREMENT EFFLUENT GROSS DAILY MAX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** ********

31/31 CALCTD 1806 03 03 0 MEASUREMENT TREATMENT PLANT Req. Mon. MGD MGD CONTI CALCTD 50050 1 0 PERMIT ******** ******** ********

REQUIREMENT EFFLUENT GROSS VALUE MOAVG NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ******** ********

0.016 0.028 19 0 20/31 GRAB MEASUREMENT 50060 1 0 PERMIT ******** ******** **** ********

0.1 0.1 MG/L FIVE PER CALCTD REQUIREMENT EFFLUENT GROSS VALUE MOAVG DAILY MAX WEEK TEMPERATURE - C, RATE OF SAMPLE ********

0.4

- 0 31/31 CALCTD MEASUREMENT 62 CHANGE CALCTD 82234 1 0 PERMIT ********

2.0 DEG ******** ******** *******

CONTI REQUIREMENT C/HR EFFLUENT GROSS DAILY MX NUOUS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certifyunder penalty of law that this document and all attachments were prepared under my" TELEPHONE DATE direction or supervision in accordance witha system designed to assure that qualified personnel Anthony L. Williams properly gather and evaluate the information submitted. Based on myinquiry of the pepgon or persons who manage the system, orthose persons directly responsible for gatherinjnhe information,the informationsubmitted is , to the best of my knowledge and belief, tiV 423 843-7001 18 11 08 Site Vice President and complete. I am aware that there are significant penalties for submittingfalse information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No closed mode operation. The following injections occurred: Flogard MS 6236 (max calc. was 0.029 mg/L, limit is 2.0 mg/L), Spectrus BD 1500 (max calc. was 0.031 mg/L, limit is 2.0 mg/L), and Spectrus CT 1300 (max calc. was 0.033 mg/L, limit is 0.05 mg/L).

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name _ JEY* ^EQ^^m^CL^I^^NT DISCHARGE MONITORING REPORT (DMR)

(SUBR01) OMB No. 2040-0004 Mdress_ _P QJBOX2000_

aNIEROFFICEOPS^lNLSON) TN0026450 101 T F - FINAL SODDY.-^AlSYj:N_37384 PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101

£acility__TVA.-^EQyOYAH NUCLEAR PLANJ Location JH/^llJpJi.TOUiiJY MONITORING PERIOD EFFLUENT From YEAR MO DAY To YEAR MO DAY

- - NO DISCHARGE Q ***

ATTN:Millicent Garland 18 10 01 18 10 31 NOTE: Read instructions before completinq this form.

X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ******** ********

- >100.0 0 3/180 COMPOS MEASUREMENT 23 CERIODAPHNIA TRP3B 1 0 PERMIT ****

PERCENT SEMI COMPOS 42.8 REQUIREMENT EFFLUENT GROSS MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ********

- >100.0 0 3/180 COMPOS MEASUREMENT 23 PIMEPHALES PERMIT ****

PERCENT SEMI COMPOS 42.8 TRP6C 1 0 ******** ******** ********

REQUIREMENT EFFLUENT GROSS MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under m] TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Anthony L. Williams properly gather and evaluatethe information submitted. Based on myinquiry of the pen^n or persons who manage the system, or those persons directly responsible for gathering tl itevice President information, the information submitted is, to the best of my knowledge and belief, true, 423 843-7001 18 11 08 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Toxicity was sampled October 8-12, 2018.

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONALPOLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name __ _IY* ^M^W^MC^EARJ^NT DISCHARGE MONITORING REPORT (DMR)

(SUBR 01) 0MB No- 2°40-0004 Address_ ^OJJOX200g_

TN0026450 103 G F - FINAL aNIERpFR^QPS^NLSQN)

SODDY_-_DAISYj:N_37384 PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Facmty _JVA-SEQUOYAH NUCLEAR PLANJ MONITORING PERIOD EFFLUENT

^c^on_JjMILTOJi_TOUJirY YEAR MO DAY YEAR MO DAY

- - NO DISCHARGE \^J ***

ATTN:Millicent Garland From 18 10 01 To 18 10 31 NOTE: Read instructions before completing this form.

X PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH SAMPLE ******** ********

6.6 7.7 0 6/31 GRAB 12 MEASUREMENT 00400 1 0 PERMIT ******** ******** **

6.0 9.0 SU ONCE/ GRAB REQUIREMENT EFFLUENT GROSS MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ********

5.8 5.9 19 0 1/31 GRAB MEASUREMENT 00530 1 0 PERMIT ******** ******** ** ********

30.0 100.0 MG/L ONCE/ GRAB REQUIREMENT EFFLUENT GROSS MOAVG DAILY MX MONTH OIL AND GREASE SAMPLE ******** ******** ********

<5.0 <5.0 0 1/31 GRAB 19 MEASUREMENT 00556 1 0 PERMIT ******** ******** ** ********

15.0 20.0 MG/L ONCE/ GRAB REQUIREMENT EFFLUENT GROSS MOAVG DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.547 0 5/31 INSTAN 1.396 03 MEASUREMENT TREATMENT PLANT Req. Mon. Req. Mon ONCE/ INSTAN 50050 1 0 PERMIT MGD ******** ******** ********

REQUIREMENT EFFLUENT GROSS MOAVG DAILY MX WEEK SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT y

")

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certifyunder penalty of law that this document and all attachments were prepared under my' TELEPHONE DATE directionor supervision in accordance witha system designed to assure that qualified personnel Anthony L. Williams properly gather and evaluatethe information submitted. Basedon myinquiry of the pers6n or persons who manage the system, or those persons directlyresponsible for gathering the- "Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 18 11 08 Site Vice President and complete. I am aware that there are significantpenalties for submittingfalse information, SIGNATURE OF PRINCIPAL EXECUTIVE includingthe possibilityof fine and imprisonmentfor knowingviolations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (IncludeFacilityName/Locationif Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 Name . TVA -SEQUOYAH NUCLEAR PLANT (SUBR01)

Address_ _P.O_BOX2000. F - FINAL aNIEROFRCE OPS^SQN) TN0026450 110 G S0DDY.-_DA]SYJ1N_37384 PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facility^ _TVA_-^EQUOYAI+/-NUCLEAR PLANJ EFFLUENT MONITORING PERIOD j£C£tjon_JdMiyPiLraUJNlJY YEAR MO DAY YEAR MO DAY

- - NO DISCHARGE [xx] ***

From 18 10 01 To 18 10 31 ATTN:Millicent Garland NOTE: Read instructions before completing this form X

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE PARAMETER OF EX TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG.

SAMPLE ********

04 MEASUREMENT CENTIGRADE PERMIT ******** ******** ** ******** ********

REPORT DEGC CONTIN CALCTD 00010 1 o REQUIREMENT UOUS EFFLUENT GROSS VALUE DAILY MX TEMPERATURE, WATER DEG.

SAMPLE ********

04 MEASUREMENT CENTIGRADE PERMIT ******** ******** ** ******** ********

30.5 DEGC CONTIN CALCTD 00010 Z 0 REQUIREMENT UOUS INSTREAM MONITORING DAILY MX TEMP. DIFF. BETWEEN SAMP. & SAMPLE ********

04 MEASUREMENT UPSTRM DEG.C PERMIT ******** ******** ** ******** ********

5 DEGC CONTIN CALCTD 00016 1 0 REQUIREMENT UOUS EFFLUENT GROSS VALUE DAILY MX FLOW, IN CONDUIT OR THRU SAMPLE ******** **

03 MEASUREMENT TREATMENT PLANT CONTIN RCORDR Req. Mon.

50050 1 0 PERMIT ******** MGD ********

REQUIREMENT UOUS EFFLUENT GROSS VALUE DAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE ******** ********

19 MEASUREMENT 50060 1 0 PERMIT ******** ******** ** ********

0.1 0.1 MG/L Five per CALCTD REQUIREMENT Week EFFLUENT GROSS VALUE MOAVG DAILY MX TEMPERATURE - C, RATE OF SAMPLE ******** **

04 MEASUREMENT CHANGE PERMIT DEGC ******** ******** ******** **

CONTIN CALCTD 2

82234 1 0 REQUIREMENT UOUS EFFLUENT GROSS VALUE DAILY MX SAMPLE MEASUREMENT

^*~

PERMIT REQUIREMENT

)

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty oflaw thatthisdocument and allattachments were prepareduhder my TELEPHONE DATE direction or supervision inaccordancewith a systemdesignedto assure thatqualified personnel Anthony L. Williams properly gather and evaluate the information submitted. Based on my inquiry o/fhe person or persons who manage thesystem, orthosepersons directly responsible for gathering the information, the information submitted is, to the bestofmy knowledge and belieJUojer^^GfaTe, 423 843-7001 18 11 08 Site Vice President and complete. I am awarethat there are significant penaltiesfor submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE includingthe possibilityof fine and imprisonmentfor knowingviolations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA ^EQ^^Hj^CLEjAR^l^rs^ DISCHARGE MONITORING REPORT (DMR)

(SUBR01) OMB No. 2040-0004 Address_ _P. a.BOX_2000.

TN0026450 110 T F - FINAL aNIERQFFICEOPS^NLSQN)

S0DDY_-_DA]SYJ1N_37384 PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facjiity_ JVA.-JEQUOYAH NUCLEAR PLANJ EFFLUENT J^catLon_ Jj/WI!JpJl(^UMT MONITORING PERIOD YEAR MO DAY YEAR MO DAY

- - NO DISCHARGE XX ATTN:Millicent Garland From 18 10 01 To 18 10 31 NOTE: Read instructions before completing this form.

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE X

PARAMETER EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ********

23 MEASUREMENT CERIODAPHNIA TRP3B 1 0 0 PERMIT ******** ******** ****

42.8

- - - - ******** PERCENT SEMI COMPOS REQUIREMENT ANNUAL EFFLUENT GROSS VALUE MINIMUM IC25 STATRE 7DAY CHR SAMPLE ********

23 MEASUREMENT PIMEPHALES TRP6C 10 0 PERMIT ******** ********

42.8 PERCENT SEMI COMPOS REQUIREMENT ANNUAL EFFLUENT GROSS VALUE MINIMUM SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT

^~~ "

PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of lawthatthis documentand allattachmentswere prepared*>>nder my TELEPHONE DATE direction orsupervision inaccordance with a systemdesigned to assure that qualified personnel Anthony L. Williams properly gather andevaluate the information submitted. Basedon my inquiry o/1heperson or personswho managethe system,orthose personsdirectly responsible for adheringthe information, theinformation submitted is, tothe best ofmy knowledge andZelief, true^^aecOrate, 423 843-7001 18 11 08 Site Vice President and complete. I am awarethatthere are significant penaltiesforsubmittingTateeHflTormation, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment forknowingviolations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved.

PERMITTEE NAME/ADDRESS (Include Facility Name/Location ifDifferent) MAJOR DISCHARGE MONITORING REPORT (DMR)

Name ___'A ^EQ^^^N^CLE^RJPLANT (SUBR01) OMB No. 2040-0004 Address_ _P. a_BOX2000_ F - FINAL (irrTEROFFICEOPS^rlSQN) TN0026450 118 G SODDY.-_DA]SYJ1N_J7384 PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER Faci!jty___ _TVA-.SEQUOYAH NUCLEAR PLANJ EFFLUENT MONITORING PERIOD J^c^oiL.Jd^liJPJLCOUJirY YEAR MO DAY YEAR MO DAY

- - NO DISCHARGE XX From 18 10 01 To 18 10 31 ATTN:Millicent Garland NOTE: Read instructions before completing this form.

X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE PARAMETER OF EX TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO)

SAMPLE ******** ********

19 MEASUREMENT MG/L TWICE/ GRAB 2

00300 1 0 PERMIT ******** ********

REQUIREMENT WEEK EFFLUENT GROSS MINIMUM SOLIDS, TOTAL SUSPENDED SAMPLE ******** ********

19 MEASUREMENT PERMIT ******** ******** **** ******** ********

100 MG/L TWICE/ GRAB 00530 1 0 REQUIREMENT WEEK EFFLUENT GROSS DAILY MX SOLIDS, SETTLEABLE SAMPLE ********

25 MEASUREMENT PERMIT ******** ******** **** ******** ********

1 ML/L ONCE/ GRAB 00545 1 0 REQUIREMENT MONTH EFFLUENT GROSS DAILY MX FLOW, IN CONDUIT OR THRU SAMPLE 03 MEASUREMENT TREATMENT PLANT ONCE/ ESTIMA Req. Mon.

Req. Mon. MGD ******** ********

50050 1 0 PERMIT REQUIREMENT BATCH EFFLUENT GROSS MOAVG DAILY MX SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT

PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

^

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify underpenaltyof lawthat this document and all attachmentswere preparejklndermy TELEPHONE DATE direction orsupervision inaccordance with a system designed toassurethat (justified personnel Anthony L. Williams properly gatherandevaluatethe information submitted. Basedon myinquipy^of the personor personswho manage the system, or those personsdirectly responsible^or gathering the information, the information submitted is, to the bestofmyknowlecWand belief, true,accurate, 843-7001 18 11 08 Site Vice President and complete. I am aware that there are significant penaltiesfor submitting falsejj SIGNATURE OF PRINCIPAL EXECUTIVE includingthe possibilityof fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

ENVIRONMENTAL RECORDS PROCESSING FORM Title of File:

SQNJDctober 2018 Semi-Annual Toxicity Report for DSN101 Site/Plant/Project Name: Accession Number (optional): Work Order Number (optional):

Sequoyah Nuclear Plant Your Name: Date Submitted (YYYYMMDD): Document Date (YYYYMMDD):

Donald W. Snodgrass 20181109 20181109 Instructions:

1) Complete the Environmental Records Processing Form.

You must include a document date on the form.

Examples:

o Annual Report = Dec 31 of year report covers (20XX1231) o Manifests - shipment date o Permit & Letters - submittal date

Verify that only one box is checked for the Document Type.

2) Save the entire document as a single PDF file with the Environmental Records Processing Form as the first page.

Name the file using the following convention: Site/Plant/Project Name_Title of file.pdf.

Notes:

o Site/Plant/Project Name - see EDMS Acronyms & Facility Abbreviations on Environment InsideNet Page for approved abbreviations Examples:

o CSC_ 2013 Annual Hazardous Waste Report.pdf o WLH_February2014DMR.pdf.

3) Email the PDF to ENVrecords@tva.gov for processing For assistance, please contact the Responsible Environmental Person for your site/project, the Environmental Media Specialists (See Contacts on Environment InsideNet Page), or your Business Support Representative.

AIR GROUNDWATER

Air Compliance Records

Groundwater Permit Compliance Records

Air Correspondence

Groundwater Permit Correspondence

Air Quality Permits & Applications D Groundwater Permits & Applications

Open Burning Permits & Records HAZARDOUS WASTE

Refrigeration Technicians Certifications - Contractors

Refrigerant Usage Logs & Surveillance Records

Hazardous Waste Correspondence

SF6 Emission Reports

Hazardous Waste Inspection Records

Hazardous Waste Permit & Applications ASBESTOS

Hazardous Waste Permit Compliance Records

Asbestos Correspondence

Mixed Waste Compliance Records

Asbestos Disposal Compliance Records

Mixed Waste Correspondence

Asbestos Medical Surveillance Records - Contractors

Uniform Hazardous Waste Manifest (UHWM), Land D Asbestos Sampling Compliance Records Disposal Restriction Notification (LDRN) Records, and

Asbestos Worker Certifications - Contractors PCB Certificates of Disposals (CDs)

Waste Characterization & Laboratory Records DRINKING WATER

Drinking Water Correspondence NATIONAL HISTORIC PRESERVATION ACT (NHPA)

Drinking Water Laboratory Results

NHPA Compliance Records

Operator Certifications - Contractors

NHPA Correspondence ENDANGERED SPECIES NEPA

Endangered Species Compliance Records

Environmental Assessments-Administrative Records

Endangered Species Correspondence

Environmental Impact Statements-Administrative PCB waste manifests and certificates of disposal are managed via the hazardous waste document type: "Uniform Hazardous Waste Manifests, Land Disposal Restriction Notification (LDRN) Records, PCB Certificates of Disposal (CDs)"

TVA 20946 [07-21-2014] Page 1 of 2

ENVIRONMENTAL RECORDS PROCESSING FORM Records *

Used Oil Shipping Papers

Non-Project NEPACorrespondence WATER/WASTEWATER OIL SPILL PREVENTION & COUNTERMEASURES

Corps of Engineer Permit Correspondence

Certificates of Applicability of

Corps of Engineer Permits &Applications Substantial Harm Criteria

Corps of Engineer Permit Compliance Records

Facility Response Plan Compliance

Injection Well Permit Correspondence Records

Injection Well Permits & Applications

Facility Response Plan Injection Well Permit Compliance Records Correspondence

National Pollutant Discharge Elimination System

OilTransfer Manual Compliance (NPDES) Correspondence Records

NPDES Permits & Applications

Oil Transfer Manual El NPDES Permit Compliance Records Correspondence

Non-Project Water Compliance

SPCC Correspondence

Sewage Treatment Compliance Records

SPCC Equipment Testing

Sewage Treatment Correspondence

SPCC Plan Compliance Records

Sewage Treatment Permits &Applications

SPCC Plans

Storm Water Correspondence

SPCC Spill Records

Storm Water Permit Compliance Records PCBS*

Storm Water Permits, Notices of Intent, &

PCB Annual Logs Applications

PCB Cleanup Records

Tennessee Aquatic Resource Alteration Permit Compliance Record

PCB Compliance Records

Tennessee Aquatic Resource Alteration Permit

PCB Correspondence Correspondence PESTICIDES Tennessee Aquatic Resource Alteration Permits &

Applications

Pesticide Applicator Certifications - Contractor

Water Quality Certification Compliance Records

Pesticide Correspondence

Water Quality Certification Correspondence

Pesticide Inventory

Water Quality Certification Permits &Applications

Pesticide Use Compliance Records GENERAL/OTHER NON-MEDIA SPECIFIC SARA TITLE III, EPCRA

EMS Correspondence

CERCLA Correspondence

EMS Documents

CERCLA Reasonable Care Evaluations

Environmental Event Reports (after Reporting to

Form R Toxic Release Inventory Supporting CDO and other required notifications)

Documentation

Environmental Audit Correspondence

SARA Correspondence

Environmental Audit Reports SOLID WASTE (INCLUDES SPECIAL WASTE)

Environmental Outlook Documents

Solid Waste Correspondence

Environmental Performance Reports (Internal)

Solid Waste Disposal Permits &Applications

Environmental Performance Reports (External)

Solid Waste Disposal Compliance Records

Regulatory Issue Summaries D Other Solid Waste Compliance Records (Special

ERALAudit Reports Waste Analysis)

ERAL Correspondence & Contracts

External Correspondence on Proposed UNDERGROUND STORAGE TANKS (USTs) Regulations or Legislation

UST Certification Forms & Certificates

Operational Control Procedures

UST Closure

Self-Assessment Reports

UST Correspondence

Utility Group Reports/Meeting Notes

UST Financial Responsibility

TVA Environmental Policy

UST Manufacturer's Information for New USTs &

Monitoring Systems

UST Monitoring & Calibration USED OIL

Used Oil Correspondence

Used Oil Sampling Results & Burn Records PCBwaste manifestsand certificates ofdisposalare managed viathe hazardouswaste documenttype: "Uniform HazardousWaste Manifests, Land Disposal Restriction Notification (LDRN) Records, PCBCertificates of Disposal (CDs)"

TVA 20946 [07-21-2014] Page 2 of 2

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: November 09.2018

1. Facility / Discharger: Sequoyah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): L579

6. Receiving Stream: Tennessee River (TRM 483.6)

7. IQ10: 3,491

8. Outfall Tested: ipj.

9. Dates Sampled: October 07 - 12.2018

10. Average Flow on Days Sampled (MGD): 1748. 1891. 1740

11. Pertinent Site Conditions: Production / operation data will be provided upon request.

12. Test Dates: October 09-16. 2018

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephalespromelas)

Daphnids (Ceriodaphma dubia)

15. Concentrations Tested (%):

Pimephales promelas: UV treated Outfall 101: 10.7. 21.4. 42.8. 85.6. 100 UV treated Intake: 100 Ceriodaphma dubia: Non-treated Outfall 101: 10.7. 21.4. 42.8. 85.6. 100 Non-treated Intake: 100

16. Permit Limit Endpoint (%): Outfall 101: IC?s = 42.8%

17. Test Results: Outfall 101: Pimephales promelas: IC25> 100%

Ceriodaphma dubia: IC25 > 100%

Page 1 of91

18. Facility

Contact:

Millicent Garland Phone #: (423)843-6714

19. Consulting / Testing Lab: Environmental Testing Solutions* Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Donald W. Snodgrass Phone #: (256) 386-2787

22. Notes: Exposures to samples collected October 07 - 12, 2018 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Page 2 of 91

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101. Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Anival Initial (MM-DD-YY)

Sample Time (ET) Time (ET) Temp. TRC* Time (ET)

ID Collected Received (°C) (mg/L) Last Used By 10-07-18 0800 to 10-09-18 1010 101 10-08-18 1530 1.2 <0.10 10-08-18 0700 10-10-18 0923 10-07-18 0800 to 10-09-18 1010 Intake 10-08-18 1530 1.1 <0.10 10-08-18 0700 10-10-18 0923 10-09-18 0800 to 10-11-18 0925 101 10-10-18 1240 5.6 <0.10 30-10-18 0700 10-12-18 0915 10-09-18 0800 to 10-11-18 0925 Intake 10-10-18 1240 2.4 <0.10 10-10-18 0700 10-12-18 0915 10-13-18 0958 10-11-18 0800 to 101 10-12-18 1320 1.1, 1.7* <0.10 10-14-18 0939 10-12-18 0700 10-15-18 0911 10-13-18 0958 10-11-18 0800 to Intake 10-12-18 1320 1.9 <0.10 10-14-18 0939 10-12-18 0700 10-15-18 0911

TRC = Total Residual Chlorine

+Samples werecollected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured bv Emperor Aquatics.

Inc.) for 2 minutes.

Page 3 of 91

Pimephales promelas Ceriodaphmadubia Test Organisms:

1. Source: In-house Cultures In-house Cultures

2. Age: < 24-hours old < 24-hours old Test Method Summary:

1. Test Conditions: Static. Renewal Static, Renewal

2. Test Duration: 7 days Until at least 60% of control females have 3 broods

3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic

4. Number of Replicates: 4 JLQ

5. Organisms per Replicate: K) i

6. Test Initiation: (Date/Time): 10-09-18 0827 ET 10-09-18 1010 ET

7. Test Termination: (Date/Time): 10-16-18 Q732 ET 10-16-18 0920 ET

8. Test Temperature: Outfall 101: Mean = 24.6°C Mean = 24.9°C (24.2-25. TO (24.7~25.2°C)

9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McICinneyville. CA).

Page 4 of 91

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephales promelas Clironic/ 7-dav Toxicity Test.

(Genus species) (Type / Duration)

Conducted October 09- 16.2018 using effluent from Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 2 3 4 5 6 7 Control, 100 100 100 100 100 100 100 UV-treated 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Control, 100 100 100 100 100 100 100 Non-treated Mean Dry Weight (mg)

Test Solutions (replicate number)

(% Effluent) i 2 3 4 Mean Control, 0.841 0.861 0.908 0.941 0.888 UV-treated 10.7% 0.749 0.876 0.936 0.890 0.863 21.4% 0.874 0.878 0.896 0.819 0.867 42.8% 0.878 0.927 0.981 0.866 0.913 85.6% 0.872 0.905 0.894 0.975 0.912 100.0% 0.766 0.909 0.913 0.952 0.885 Intake 0.972 0.903 0.847 0.815 0.884 Control, 0.840 0.878 0.949 0.875 0.886 Non-treated IC25 Value: > 100% Calculated TU Estimates: < 1 .0 TUc*

Permit Limit: 42.8%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LCso: TUc = 100/ IC25 Page 5 of 91

TOXICITY TEST RESULTS (seeAppendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Cluonic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted October 09- 16. 2018 using effluent from Outfall 101.

Percent Surviving Test (time interval used - days)

Solutions 1 2 3 4 5 6 7

(% Effluent)

Control 100 100 100 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Reproduction (#young/female/7 days)

Test Solutions Data (replicate number)

(% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 31 34 28 27 32 27 31 33 32 30.4 10.7% 32 33 35 30 37 36 30 37 38 34.1 21.4% 34 39 35 35 34 36 35 39 23 35.3 42.8% 35 33 40 39 34 31 39 38 40 36 36.5 85.6% 35 36 41 37 40 34 37 41 37 38 37.6 100.0% 38 37 41 39 41 37 40 37 39 37 38.6 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 42.8%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA TUa = IOO/LC50: TUc = 100/ IC25 Page 6 of 91

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-dav Toxicity Test.

(Genus species) (Type / Duration)

Conducted October 09- 16,2018 using water from Intake Percent Surviving Test (time interval used - days)

Solutions 1 2 3 4 5 6 7

(% Effluent)

Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7 days)

Test Solutions Data (replicate number)

(% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 27 31 27 32 30 28 29 29 29 29.2 Intake 37 38 36 34 37 38 38 34 35 35 36.2 ICzs Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = IOO/LC50: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC25)

Pimephales promelas October 09- 16, 2018 0816 7 days KC1 0.67 g/L Ceriodaphnia dubia October 09- 16,2018 0947 7-days NaCl 1.06 g/L Page 7 of 91

PItVSlCAUCUKMICAL

SUMMARY

Water Chemistry Mean Values and Ranges Ibr UV-treated Pimephales promelas and Non-treated Ceriodaphma dubia. Seqiioyuh Nuclear Plant (SQN). Effluent OmlaJI 101 and Intake performed October 09* 16? 2018.

Test Sample ID Temperature (°C) Dissolved Oxygen (mg/L) pit (S.D.) Conductance Alkalinity Hardness *Totnl Residual Initial Final Initial Final Initial Final (/t mhos/em) (mgflLCaCOj) (mg/LC&COj) Chlorine (mg/L)

Control. 24.7 24.4 7.8 7.6 7.62 732 309 59 81 -

Nan-fti't>>M 24.6 - 24,7 243 - 24.5 7 7 - 8.0 6.6 - 79 7,49 - 7.75 7.06 - 7.70 297 - 319 58 - 60 80 - 84 Control. 24.7 24.6 7.9 7.8 7.70 7,34 307 59 81

UV-itcitlrd 247 - 248 244 - 24.7 7.8 - 8.1 7.1 . 8.1 7.46 - 7.87 7 13 - 7.71 295 - 318 59 - 59 80 - 82 24.8 24.5 8.0 7.7 7.69 7,30 294 10.7%

i 247 - 24,9 24.3 - 246 7.9 - 8.1 7.0 - 8 1 748 - 7 87 709 - 769 276 - 314 24.8 24.4 8.1 7.7 7.68 7.30 275 21.4% - -

24 7 - 24.9 243 - 24.6 7.9 - 8,2 6 8 - 8.1 7.06 - 7.68 264 - 291 1 24.8 24.5 8.1 7.7 748 -

7.66 7 86 7.29 240 42.8% - - -

24.7 - 25.0 243 - 24,6 80 - 8.2 6.7 - H2 7.48 - 7.84 7.06 - 7.65 231 - 250 24.9 24.5 8.1 7.8 7 61 7.27 170 85.(i% - * -

24.7 - 25.0 24,2 - 24.7 8.0 - 8.3 6.7 - S.2 7.46 - 780 6.97 - 7.62 162 - 179 24.9 24.4 8.2 7.8 7.58 7.21 149 53 53 <0,10 100%

24.8 - 250 24.2 - 247 8.1 - 8.3 6.8 - 8.2 7.44 - 7.79 6.96 - 7.59 137 - 160 50 - 54 52 - 54 <0 10 - <() to 25.0 24.5 8.2 7.8 7.55 7 17 143 53 55 <0J0 Intake 24,7 - 25.1 24.3 - 247 8.0 - 8.4 6,7 - 8,3 7.43 - 7.80 6.92 - 7.53 135 - 158 52 - 56 52 - 58 <0 10 - < 0.1.0 Couirol, 24.7 25.0 7,8 7.9 7.62 7.64 309 59 81 -

Noim rented 24.7 - 248 248 - 25,2 7.7 - 7.9 7.8 - 8.0 7,49 - 7.75 7,40 - 7.82 297 - 319 58 - 60 80 - 84 24.8 24.9 7,9 8,0 7.73 7.63 291 10.7% - -

24.7 - 249 248 - 25,1 7 7 - 8.1 7.9 - S.l 7.50 . 7.86 74! - 7.81 279 - 308

,<<2 24.8 24.9 7.9 8.0 7J0 7.62 272 21.4% - -

24.7 - 24 9 24.8 - 25,1 77 - 81 7.9 - 8.1 7.48 - 7.83 7.42 - 7.80 262 - 285 24.8 24.9 8.0 8.0 7.66 7.60 239 42.8% - -

247 - 25.0 24.7 - 25.1 7,7 - 8.2 80 - 8.1 745 - 781 7 41 - 7.79 232 - 249 ft 24.9 25.0 8.0 8.1 7.58 7.57 170 85.6% - -

24.8 - 25.0 248 - 25,2 7.8 - 8.2 7 9 - 8.1 739 - 775 7.40 - 7.75 160 - 177 24.9 24.9 8.1 8.1 7.54 7-55 142 52 53 <0.10 100%

247 - 25.0 24.8 - 25.0 7.8 - 8.2 7.8 - 8.2 7.36 . 7.73 7.35 - 7.74 134 - 148 50 - 54 50 - 54 < 0 10 - <0.I0 24.9 24.9 8.1 8.1 7.53 7.53 141 53 55 <0.IO intake 24.7 - 25.0 248 - 25, t 7.8 - 8.3 7.8 - 8.3 736 . 776 732 - 7.74 134 - 148 52 - 56 52 - 58 <0.10 - <0.10

Nolc. Total residual chlorine was performed onnon-trcaicd Outfall 101 and Intake samples Overall1c nine nilure(°C) Ave rage (VI minium M axinmm Pimephales promelas 24.6 24.2 25!

Ceriodaphnia dubia 24.9 24.7 25.2

SUMMARY

/ CONCLUSIONS-Exposuresto samples collected October07 - 12,2018 from. Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >100 percent Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Page 9 of 91

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas Samples used in the fathead minnow test were exposed to UV light for two minutes prior to introduction of test organisms. UV treatment is used to control interference offish pathogens. This treatment method was approved on November 23, 2015 by the State of Tennessee in a letter from Jessica Murphy to Terry Cheek, Senior Manager of TVA Water Permits, Compliance, and Monitoring.

2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

2. Ceriodaphnia dubia None Page 10 of 91

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expimtion dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B-2010.

4. Dissolved oxygen was measured by SM 4500-O G-201L

5. The pH was measured by SM 4500-H+ B-2011.

6. Conductance was measured by SM 2510 B-2011.

7. Alkalinity was measured by SM 2320 B-2011.

8. Total hardness was measured by SM 2340 C-2011.

9. Total residual chlorine was measured by ORION 97-70-1977.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCl.

2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 11 of 91

REFERENCES

1. NPDES Permit No. 1TM0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms. EPA-821-R-02-013 (October 2002).

j>. Standard Methods for the Examination of Water and Wastewater. 22nd Edition. 2012.

4. Quality Assurance Program: Standard Operating Procedures. Environmental Testing Solutions, Inc (most current version).

Page 12 of 91

Sequoyah Nuclear Plant Biomonitoring October 09-16.2018 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 13 of 91

Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat TRC EO/PO 02/06/2005 -

<0.0042 0.028 0.010 _ .

02/07/2005 -:; <0.0116 0.028 0.010 0.007 02/08/2005 -

<0.0080 0.028 0.010 _

02/09/2005 -

0.0199 0.028 0.010 <<, .

02/10/2005 -: <0.0042 0.028 0.010 _ .

02/11/2005 - 0.0155 0.028 0.010 - -

- 0.007 -

06/05/2005 -

0.0063 . _ _

06/06/2005 0.0043 0.037 06/07/2005 0.0103 - .

0.037 06/08/2005 0.0295 -

0.037 06/09/2005 -

0.0129 - _ _ . .

06/10/2005 - 0.0184 - -

07/17/2005 -

0.0109 0.026 0.009 _ _

07/18/2005 0.0150 0.026 0.009 0.036 07/19/2005 0.0163 0.026 0.009 0.036 07/20/2005 0.0209 0.026 0.009 0.014 0.036 07/21/2005 -

0.0242 0.026 0.009 _ _

07/22/2005 - 0.0238 0.054 0.018 - - -

0.014 -

10/30/2005 -

0.0068 . _ _

10/31/2005 -

0.0112 _ _

11/01/2005 0.0104 0.035 11/02/2005 0.0104 0 036 11/03/2005 0.0117 0.036 11/04/2005 - 0.0165 - " - -

- - 0.035 11/14/2005 -

0.0274 - _ _ .

11/15/2005 -

0.0256 - _ .

11/16/2005 -

0.0234 _ _ _

11/17/2005 -

0.0231 _ _ _

11/18/2005 -

0.0200 _ _

11/19/2005 - 0.0116 - - - - - -

Page 14 of 91

Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M MSW Hypochlorite mg/L mg/L mg/L mg£L mg/L mg/L 73551 mgft 101 mg/L TRC Phosphate Copolymer DMA© Azole Quat mg/L <$uat mg/L TRC EO/PO Phosphate 11/12/2006 0.0055 - - - * - - -* -

., V.:"> -

11/13/2006 -

0.0068 *- - ' - -

- daS7 -

11/14/2006 * - 0.0143 - - - - - -

0.037 -

11/15/2006 -

0.0068 '- -

0,037 -

11/16/2006 -

0.0267 - - - -

"0:837 -

11/17/2006 - 0.0222 - ? - - - - -

11/26/2006 -

0.0188 - -

11/27/2006 '* r 0.0138 -

V - * - - - -

11/28/2006 -.

0.0120 - - - - -

r. -

11/29/2006 -

0.0288 - - - - - - - -

11/30/2006 *- 0.0376 - - .- - -

12/01/2006 > 0.0187 " -

05/28/07 > * - - - -' - -. -

0.015 05/29/07 - - - - - * - - -

&036 0.015 05/30/07 - 0.0084 - - - - -

0.017 oteo^s 0.015 05/31/07 -

0.0103 - - * - - - -

0.036 0.015 06/01/07 -

0.0164 - - - * - --

0.017 0.036 0.015 06/02/07 .' *- 0.0305 " ."* - - * - - - 0.015 12/02/07 -' 0.0241 * - -

. -* '^ - - - " -' - -

12/03/07 -

0.0128 - - - - -

^ -*'-*

12/04/07 . '. -* ' 0.0238 - '** -. - ;- -

'*w \' -

12/05/07 '- 0.0158 "

12/06/07 -

0.0162 - - - - - - - -

12/07/07 0.0175 - *; - - -

04/13/08 0.0039 - " - - ' - ' ' " - - -

04/14/08 -

0.0124 * - - - - - . -

04/15/08 . .

0.0229 - - - - - - - -

04/16/08

0.0143 - - - - - . - _

04/17/08 -

0.0120 - - - - - . . _

04/18/08 - 0.0149 - - - - - - - -

10/26/08 *- 0.0260 - - . _ - . _ _

10/27/08 -

0.0151 - - - - .

0.017 . .

10/28/08 - 0.0172 - - - - - -

0.041 _

10/29/08 0.0154 ' - - - -

0.018 0.041 0.030 10/30/08 - - - - - - -

0.041 0.030 10/31/08 "' - 0.0086 - - - - - - 0.041 0.030 Page 15 of 91

Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg/L Quat mg/L TRC Phosph EO/PO Quat Phosphate ate 02/08/09 -

0.0197 - - . - .

0.017 _ _ _

02/09/09 - 0.0237 - - . - .

0.017 _ _ _

02/10/09 - 0.0104 - - - . .

0.021 _ _

02/11/09 - 0.0155 - - - - .

0.017 _ _ _

02/12/09 -

0.0106 - - - - .

0.017 _ _ _

02/13/09 - - - - - - - - - - -

05/10/09 -

0.0129 - - . _ _ _ _ _ .

05/11/09 - 0.0415 - - - - - . .

0.0446 _

05/12/09 - 0.0053 - - - - . - .

0.0396 _

05/13/09 - 0.0049 - - - - - - .

0.0396 _

05/14/09 - <0.0141 - - - - . - .

0.0397 -

05/15/09 - <0.0160 - - - - - - - - -

11/15/09 -

0.025 - - - - . _ _ _

11/16/09 -

0.0152 - - - . _ _ _ _ _

11/17/09 -

0.0255 - - . - . _ _ _ _

11/18/09 -

0.0306 - - - - . _ .. _ _

11/19/09 - 0.0204 - - . . _ _ _

11/20/09 - 0.0093 - - - - - - - - -

05/09/10 -

0.0192 - - - - _ _ _ _ _

05/10/10 -

0.0055 - - . . _ _ _ _ _

05/11/10 - 0.0100 - - - - . -

0.039 _ _

05/12/10 - 0.0171 - - - - . .

0.039 _ _

05/13/10 -

0.0041 - - - . -

0.039 _ _

05/14/10 - 0.0099 - - - - - - 0.039 - -

Page 16 of 91

Date Sodium Towerbrom PGL- PCL-401 GL-363 Cuprostat msoM Nalco Spectrus H-150M W&1&. Floguard Hypo mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L l;lll;:,l MS6236 chlorite TRC mg/L Copoly DMAD Azole Qtfat mg/L mg/L Quat Jitflfc; mg/L mg/L Phos mer EO/PO Quat l^ffi Phosphate TRC phate 10/31/10 - - - - - - - - -

7f:*'%:; * -

11/01/10 0.0122 . - - - .- - -- - -

M-m--**'* -

11/02/10 .N- 0.0112 - * -* - - * "- -

lllll:-;;..'-^ -

11/03/10 -

0.0163 - - - - - - - jK'*7-'/ -

11/04/10 .<< 0.0107 - - - - - - - -

--M+ V'* -

11/05/10 - 0.0132 ' - -

/* -' - - ;; - -

WM-&: -

05/01/2011 - - - - . - - - - -

05/02/2011 - - - - -', -

0.04 - ]^^;*.:J. -

05/03/2011 -*' - - - -* - -

0.04 -

mm^-: -

05/04/2011 0.0155 - - - - - -

0.04 -

V'?:***.*'" -

05/05/2011 -

0.0179 - - - * - - -

0.04 - V-j,, V.-y/;] -

05/06/2011 0.0089 * - . - - . - -' - - -

wmm -

11/06/2011 -' 0.0168 - - - - '- - - -

V:^:~\ -

11/07/2011 -- 0.0225 -. - - - - - - *- - '*::>%- -* I -

11/08/2011 -~ 0.0141 *-

'^ff§*.y-^;J -

11/09/2011 -

0.0239 - - - - - - "*- -

KHJ;; " -

11/10/2011 -

0.0242 - * - - - #- - - -

^&'-:?. ,' C -

11/11/2011 *' *'_-!* 0.0231 :-.. - * -

05/06/2012 - - - - - - *- -

05/07/2012 - - . - - - - - - - -

^fev^v'-Vi -

05/08/2012 ".-. -

~

0.041 - lil/JSl:iR.J;'^ -

05/09/2012 * - ' 0.0145 - - - - - -

0.041 - \:S^\:y&^'['*'*;.] -

05/10/2012 0.0298 - - - - - -

0.041 -

^&4^\^ -

05/11/2012 .- 0.0174 - - - -. - -

llil';:l -

08/12/2012 - - - - - '- -

^*0&* -.:*": 0.029 08/13/2012 ;- 0.0256 - - - - -

0.028 0.037 -

t^,f:'-:^:- 0.029 08/14/2012 * ',

0.0209 - - * .- - **- -

0.037 -

lififet.-.^ 0.029 08/15/2012 - 0.0279 - - - - -

0.028 - : -

'W:'j* ' ':"* 0.029 08/16/2012 -

0.0076 -

r~:~y~::. \. :* 0.029 08/17/2012

'i 0.0446 - - - - ,. - -' .. - - -

IfliSS 0.032 05/12/2013 '- 0.0099 - - - - . - . .

'}lWk-:'**:'*. .

05/13/2013 .- . * - - - - - - -

3.^:y-^';" '**/*/ 0.064 05/14/2013 0.0091 . - - - - - -. 0.039 -* -

IXSl#45^ 0.064 05/15/2013 - , ' 0.0096 - - '- - -

0.039 - > -

?'V: ^:i.'.: *'*: 0.064 05/16/2013 -

0.0229 - - - - . - . .

^;.i- - ' 0.032 05/17/2013 0.0063 -

illl;:,.;:£ 0.032 09/15/2013 - - - - - - - - . - .

R?I;'^:--';'**!** : 0.03 09/16/2013 -

0.0072 - - - - . -

0.0379 . ;;v/;-';: :m .*': 0.03 09/17/2013 ~ 0.0107 * - - ' *-.' ; - -*""', - 0.036 0.03^9 - tM0£/y \;:{r 0.03 09/18/2013 ;-- 0.0217 - - - - . --..- 0.036 010379 - Wfp+/- -* *;*: 0.03 09/19/2013 *.- 0.0172 .-. - *- ., - *- - . -

\ -"-;' ' 0.03 09/20/2013 ,*- 0.0173 - \ - - - - - , . -

W^-s 0.03 Page 17 of 91

Date Sodium Towerbrom PCL- PCL-401 Cuprostat CL-363 H-130M Nalco Spectrus H-150M MSW Floguard Hypo mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly DMAD Azole Quat mg/L mg/L Quat mg/L mg/L mg/L Phos mer EO/PO Quat Phos Phosphate TRC phate phate 05/04/2014 -

0.0118 . . _ >> . .

05/05/2014 -

0.0112 . _ _ _ _

05/06/2014 -

0.0096 . _ _ _ _ .

05/07/2014 -

0.0164 - . _ _

05/08/2014 -

0.0235 . - . _ _ _ _

05/09/2014 - 0.0110 - - - - - " - - - -

09/07/2014 - - - - - _ _ _

09/08/2014 - - - - - _ -

0.04 . .

09/09/2014 - - - - - _ _

0.04 .

09/10/2014 - - - - - _ - _

0.04 _ .

09/11/2014 -

0.0070 - - - _ _ _ _ .

09/12/2014 - 0.0074 - -

08/09/2015 -

08/10/2015 0.0195 0.03 08/11/2015 0.0275 0.03 08/12/2015 0.0213 _

0.03 08/13/2015 0.0192 _

0.03 08/14/2015 0.0182 - 0.03 10/18/2015 0.0162 10/19/2015 0.0125 10/20/2015 0.0120 10/21/2015 0.0130 10/22/2015 0.0174 10/23/2015 0.0156 05/15/2016 -

05/16/2016 0.0209 05/17/2016 0.0210 05/18/2016 0.0361 05/19/2016 0.0254 05/20/2016 0.0261 07/31/2016 - -

08/01/2016 0.0091 0.03 08/02/2016 0.0093 0.03 08/03/2016 0.0209 0.03 08/04/2016 - _

08/05/2016 -

04/30/2017 -

05/01/2017 0.0298 05/02/2017 0.0218 05/03/2017 0.0260 05/04/2017 -

05/05/2017 -

Page 18 of 91

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Sequoyah NuclearPlant Bioraonitoring October 09-16,2018 Appendix C Chain of Custody Records and ToxicityTest Bench Sheets Page 20 of 91
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1_
Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):
4>
roject Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client^
XX-
6.0. Number
N/A Asheville, NC Other (specify): CbnWcW 3ti
<o General Comments:
facility Sampled: Sequoyah NP 28801 101 Bottle Comp 0*6$° NPDES Number: TN0026450 Phone: 828-350-9364 Collected &j A£lW> &bU*Ht Avv)h tk*W** Fax: 828-350-9368 INT Bottle Comp @0?°$
j/u Field identification / Grab/Comp a Flow Collection Date/Time Container Sample Description Number & (MGD) Rain Event?
Laboratory Use Volume (Mark as Appropriate)
Collected Date Timu BTS Log Arrival Temp, By Time Appear (mro/dd/yy) (EST) Number ance SQN-I01-TOX Comp Start lO-I'VZ 0?oo 10IO06.0Z f.a c ^ /S30 -#
End iJ-£- \$
OlOO I (2.5gal)
P%$m SQM-INT-TOX Comp Start 10-">-<*
0$° o
\Bmd.o3 Ire ^ /S>o
End lot- [$
Q7QO 1 (2.5 gal) -f Custom \ SeAti u>r*cr. Siv^'ues
>>/(\
Sample Custody - Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): $& Date/Time
-tvft iQ'f-t?/ \\\S 6.1- lo-r-ir / infer iaj]Jg£§fr ST
££&&} \Cfifc>M*l
Sw*
^e>M 10-^'i? / JrJ^sr /0-OS-tS /SSo er TVIfv CDtJXftACS>>*_
Whole Effluent Sample Receipt Log Page H^
Sample temperature performed usingSample Receiving Thermometer: SN160928622 s Date Received Time Received Received by Received from
Sample Project Sample Sample name and description State Comments CD Temp. (DC) number number 10-08-18 1200 K, Keenan W.Ladd 3.4 13632 181008 .01 Andrews WWTP - Pp NC 10-08-18 1530 K. Keenan A. Panter 1.2 13633 181008 .02 TVA - Sequoyah Nuclear Plant -101 O TN 10-08-18 1530 K, Keenan A. Panter 1.1 13633 181008 .03 TVA - Sequoyah Nuclear Plant ** Intake TN SOP G4 - Exhibit 64.2, revision 01-03-12
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1_
^jient: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):
-0) roject Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client g.0. Number: N/A Asheville, NC Other (specify): £on*C 28801 General Comments:
facility Sampled: Sequoyah NP 101 Bottle Comp @ CH&5 NPDES Number: TN0026450 Phone: 828-350-9364 Coiled*, Mb *&"£<- J&> ^^ Fax: 828-350-9368 INT Bottle Comp @ cn^
Field Identification / Grab/Comp Collection Date/Time Container Flow Sample Description Rain Event?
Number & (MGD) ^ Laboratory Use Volume (Mark as Appropriate)
Collected Sample Custody- Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time
£0>>&C *be<<~*<<4H
\oi\M 3 of&*£R$&L /o^/ o%'33*x tffl J££j2^ S£- /cW&V* iXHb\ 4 er /0-fO-<t ix^o *-T
rirftt Page 1Z1 Whole Effluent Sample Receipt Log
Sample temperature performed using Sample Receiving Thermomeierr SN 160928622 Date Time Received Received Sample Project 1 Sample Sample name and description State Comments Received Received by from Temp. (*C) number number 10-10-18 0931 K.Keenan Fed-Ex 1.8 13645 181010 .01 ALCOA -005 NC 10-10-18 0931 K.Keenan Fed-Ex 1.8 13646 181010 .02 ALCOA-012 NC 10-10-18 0931 K. Keenan Fed-Ex 1.8 13647 181010 .03 PCSPhosphate, Inc NC
<o 10-10-18 1042 K, Keenan Fed-Ex 1,6 13648 181010 .04 Carv/ApexWTF NC 10-10-18 1042 K. Keenan Fed-Ex 2.4 13649 181010 .05 Ctarkton WWTP NC 10-10-18 1042 K. Keenan Fed - Ex 2,4 13650 181010 ,06 Cherry Point NC 10-10-18 1042 K. Keenan Fed - Ex 1.4 13651 181010 ,07 Ounn WWTP NC 10-10-18 1042 K. Keenan Fed - Ex 2.3 13652 181010 .OS f armville WWTP NC 10-1048 1042 K. Keenan Fed - Ex 2.4 13653 181010 .09 Frankllnton WTP NC 10-10-18 1042 K. Keenan Fed - Ex 2.4 13654 181010 .10 Hamlet WWTP NC 10-10-18 1042 K. Keenan Fed - Ex 2.2 13655 181010 .11 Greenville WTP NC 10-10-18 1042 K. Keenan Fed-Ex 1.2 13656 181010 .12 GoldsborcWRF-001 NC 10-10-18 1042 K. Keenan Fed-Ex 2.4 13657 181010 .13 Hermitage House WWTP m 10-10-18 1042 K. Keenan Fed-Ex 1.7 13658 181010 .14 Marshall SS NC 10-10-18 1042 It Keenan Fed-Ex 1.4 13659 181010 .IS Mayodan WWTP NC 10-10-18 1042 K. Keenan Fed-Ex 2.4 13660 181010 .16 Mt. Olive WWTP NC 10-10-18 1042 K. Keenan Fed-Ex 1.4 13661 181010 .17 Newport WWTP NC 10-10-18 1042 K. Keenan Fed - Ex 2.2 13662 181010 ,18 Pasquotank WTP NC 10-10-18 1042 K. Keenan Fed-Ex 2.4 13663 181010 ,19 Princeton WWTP NC 10-10-18 1042 K. Keenan Fed-Ex 1.6 13664 181010 ,20 Radiator Specialty NC 10-10-18 1042 K. Keenan Fed - Ex 1.7 13665 181010 .21 M'KeanMaffitWWTP NC 10-10-18 1042 K. Keenan Fed - Ex 2.4 13666 181010 .22 Smlthfleld Packing NC 10-10-18 1042 K.Keenan Fed - Ex 2.8 13667 181010 23 South Mills WTP NC 10-10-18 1042 K.Keenan Fed - Ex 1.5 13668 181010 .24 Greenville WWTP NC 10-10-18 1042 K.Keenan Fed-Ex 2.4 13669 181010 .25 Bay Valley Foods NC 10-10-18 1042 K.Keenan Fed - Ex 1.6 13670 181010 .26 Mt. Olive Pickle Company NC 10-10-18 1042 K.Keenan Fed-Ex 2.4 13671 181010 .27 Worsley NC 10-10*18 1042 K.Keenan Fed-Ex 1.1 13672 181010 .28 TVA-Lagoon Greek CC-Outfall 001 TN 10-10-18 1042 K.Keenan Fed - Ex 1.3 13672 181010 .29 TVA - Lagoon Creek CC- Well Water TN 10-10-18 1109 K, Keenan W. Ladd 2.0 13632 181010 .30 Andrews WWTP -Pp NC 10-10-18 1240 J. Sumner TVA Courier 5.6 13633 181010 ,31 TV/p- Sequoyah Nuclear Plant -101 TN 10-10-18 1240 J. Sumner TVA Courier 2.4 13633 181010 .32 TVA - Sequoyah" Nuclear Plant- Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12
Whole Effluent Sample Receipt Log Page H/S
Sampletemperature performedusingSampleReceiving Thermometer: SW 160928622 Date Time Received Received '"Sample Project Sample Received Received from Sample name and description State Comments by Temp.fC) number number 10-12-18 0951 K. Keenan Fed-Ex 1.6 13648 181012 .01 Cary/Apex WTF NC 10-12-18 0951 K. Keenan Fed-Ex 2.0 13649 181012 .02 ClarktonWWTP NC 10-12-18 0951 K. Keenan Fed - Ex 2.0 13650 181012 .03 Cherry Point NC
<o 10-12-18 0951 K. Keenan Fed - Ex 2.7 13651 181012 .04 Dunn WWTP NC 10-12-18 0951 K. Keenan Fed - Ex 2.4 13652 181012 .05 Farmville WWTP NC 40-12-18 0951 K. Keenan Fed - Ex 1.6 13653 181012 .06 Frankllnton WTP NC 10-12-18 0951 K. Keenan Fed - Ex 1.6 13654 181012 .07 Hamlet WWTP NC 10-12-18 0951 K. Keenan Fed - Ex 2.4 13655 181012 .08 Greenville WTP NC 10-12-18 0951 K. Keenan Fed-Ex 2.1 13656 181012 .09 GoldsboroWRF-001 NC 10-12-18 0951 K. Keenan Fed - Ex 2.0 13657 181012 .10 Hermitage House WWTP NC 10-12-18 0951 K. Keenan Fed-Ex 1.9 13658 181012 .11 Marshall SS NC 10-12-18 0951 K. Keenan Fed - Ex 2.5 13659 181012 .12 Mayodan WWTP NC 10-12-18 0951 K. Keenan Fed - Ex 2.0 13660 181012 .13 Mt Olive WWTP NC 10-12-18 0951 K. Keenan Fed-Ex 3.5 13661 181012.14 Newport WWTP NC 10-12-18 0951 K. Keenan Fed-Ex 2.0 13663 181012 .15 Princeton WWTP NC 10-12-18 0951 K. Keenan Fed-Ex 2.2 13664 181012 .16 Radiator Specialty NC 10-12-18 0951 K. Keenan Fed - Ex 1.8 13665 181012 .17 M'KeanMaffitWWTP NC 10-12-18 0951 K. Keenan Fed-Ex 2.0 13666 181012 .18 Smlthfleld Packing HC 10-12-18 0951 K. Keenan Fed-Ex 2.0 13669 181012 J 9 Bay Valley Foods NC 10-12-18 0951 K. Keenan Fed - Ex 1.7 13670 181012 .20 Mt. Olive Pickle Company NC 10-12-18 0951 K. Keenan Fed - Ex 1.4 13668 181012 .21 Greenville WWTP NC 10-12-18 1250 K. Keenan W.Ladd 0.6 13632 181012 .22 Andrews WWTP -Pp NC 10X12-18 1320 J. Sumner TVA Courier 1.1/1,7 13633 181012 .23 TVA -Sequoyah Nuclear Plant -101 TN 10-12-18 1320 J. Sumner TVA Courier 1.9 13633 181012 .24 TVA - Sequoyah Nuclear Plant - Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12
Page I of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas Client: Tennessee Valley Authority County: Hamilton Facility: Sequoyah Nuclear Plant Outfall: 101 NPDES #: TN0026450 Project*: rSfalft Dilution preparation information: Comments:
Dilution prep (%) 10.7 21.4 42.8 85.6 100 Each concentration was UV-treated Effluent volume (mL) 267.5 535 1070 2140 2500 for 2 minutes to remove pathogenic Diluent volume (mL) 2232,5 1965 1430 360 0 Interferences.
Total volume (mt) 2500 2500 2500 2500 2500 Test organism information: Test information:
Organism source: In-house culture Randomizing template: kuoe-Age: < 24-hours old Incubator number and shelf location: 1C Spawn date; W-CrArlfc Anemia CHM number CHM984 Hatch dates and times: Drying information for weight
""TO I0-OV14 QtoQO determination:
Transfer vessel information: pH= a so S.U. Date / Time in oven: 16-l.lrl* <n4S Temperature = lH>\ °C Initial oven temperature: feG-C Average transfer volume: < 0.25 mL Date / Time out of oven: I5>>t1~t( OlM£ Final oven temperature: urc Total drying time: 1H-AOA&
Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake
\g /Oftf-CO.
4- (00W 10-09-18 of^"^ WlOOif-Ck ObuS y\Q%
10-10-18 QfaOQ
QJD0
-&- OSi\ X \& to Otf-gL lgfOOf-fifr fo-<S-tf IO-il-18 tdSfr* ,**
oftiS
IV- lJHOiD^\ lg*0i&*VL. io-<rvil 10-12-18
& mo
i&ioib.Si 10-13-18
_QfetO_
. ixfo
-*- QtTL-4- itioio/b'x io-ovig
\%\x>\\. xs>
CTTDCi
^ W>0 0^'T-
¥<*
ICIDIX-XA lo-U-if ^
10-14-18 tD*v\-lf A
-&- uipq-is \gtDtt.vt 10-15-18 Cfloo \>>>o&
£ <HV\
04 tl KM6-18 QloOO 4- rLift
- **- *£tt> n,-13> iSlotl.ttt *b-H-ft£A cftM--
1 &*T Acceptance criteria Control information: *jH&d* OV*~ TtUATCfc Summary oftest endpoints:
% Mortality: 07. <20% 7-day LCso > I0&1.
Average weight per initial larvae: o.&SS- NOEC 1007.
Average weight per surviving larvae: 0. 6&S > 0.25ma/larvae LOEC >iooi ChV >I007.
1C25 >i0O7.
SOP AT20 - Exhibit AT20.3, revision II-0I-I4 Page 27 of 91
\rrnz=m^
3C nut tnCK^i (Uinx^
Page 2 of7 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, UV-treated Date: 10-09-18 Survival and Growth Data Day l CONTROL 1 10.7% 21.4%
A B c O 1 E F G l H I j K L 0
10 10 10 10 10 10 10 10 10 10 10 10 1
Ifc i0 (0 ID /D ID <D /o /o '6 '0 '0 2
(Q 10 >>0 10 10 >>0 ID to 10 '0 <<0 /o 3
10 ID 10 £0 10 to to to '0 <D ID I 10 4
'D 10 10 ID to ID to ID 10 to (Q to 5
ID 10 ID 10 10 10 to to ID '0 to to 6
Id 10 <0 lO to to 10 <<o to to (Q to
/o fb '0 '0 fO ib /o to 70 /o 10 '0 7
A = Pan weight (mg)
Tray color code:: fokk/
Analyst: TS ' 15.90 13.35 /5J7 /<</.H0 15.<< )H.n 13.7(3 15.7? X53\ I3.S6 13.66 13.77 Date: Qft.3fo-tt B << Pan + Larvae weight (mg)
Analyst: A( 7rt.ii 1\A^ *>>WS 2*S\ 11.^ ivM ^^.cfe T£V\ ii\M w.rt 1-L.Sfe 26.%
Date: f&*t"Vi&
C = Larvae weight (rag) ~ B - A Hand calculated. .
&Mi S.bV <\M VU Vtt &.1U V<<" lAO &M sag &.<Uo g.*4 Analyst: $
Weight per initial number of larvae (nig)
= C / Initial number of larvae Hand calculated.
Analyst:
\ v s* 0#
dv o 1v 0* 0" 0*
Average weight per Percent reduction c-j^. ,.
initial number of from control (%) o.US. 0.8 v>>*>> 2-&7. o.&tn 7.m.
larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k - killed, m - missing* sk = sick, sm = unusually small, Ig = unusually large, d&r - decanted and returned, w = wounded.
Comments:
SOP AT20- Exhibit AT20.3, revision 11-0I-I4 Page 28 of 91
Pa*e 3 of 7 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant OutfaU 101, UV-treated Date: 10-09-18 Survival and Growth Data Day 42.8% 85.6% 100%
M N O p Q R S T U V w X 0
10 10 10 10 10 10 10 10 10 10 10 10 I
ffc <b 10 to ib to to to to to to ID 2
<<D (0 10 to 10 to ID to '0 '0 /o 10 3
>>b 10 10 10 '0 1Q 10 to to 10 10 to 4
10 10 10 J0 10 to 10 to to to 10 to 5
10 10 (O 10 10 to lO tb 10 to >>0 ID 6
to '0 >>0 10 to <<0 to to to 10 10 '0 7
lO 10 /e> lb to lb /O /o to to to to A << Pan weight (mg)
Traycolor code:: fOo^
Analyst: TS 15.00 15-75 15M \5m 15.73 IW IH.86 \&n [5.U 11.7;? ISM 15.TS Date: 6&Xt-l%
B - Pan + Larvae weight (mg)
Analyst: X\
7>>m Ti\.*fl ts.<& TH-lfc ulM* TA& i%&b 2s.m v^s Ti.Sl TM.il TS.S0 Date: ^H-H-l*
C = Larvae weight (mg)= B - A Hand calculated. *.i& %r\ <uv a.wi &.~u *.<& SM <\.m -i.u>> I.Crt °>>.>>b *i.-si.
Analyst: \\
Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculated. J9 OS?
Analyst: Y\ O' 0' 0' 0' Average weight per Percent reduction initial number of from control (%) 0.4'^h "2 .4 7. 0.<=\\TL -1.17. o.s&s 0.2>7.
larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
SOP AT20 - Exhibit AT20.3, revision 11-0I-I4 Page 29 of 91
Paae4of7 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant Outfall 101, UV-treated Date: 10-09-18 Survival and Growth Data Day 100% Intake l Control- Non-treated Y z AA BB l CC DD EE FF GG HH n jj 0
10 10 10 10 10 10 10 10 1
Ib to to to *0 '0 to '0 2
10 10 10 '0 >D '0 /o to 3
10 10 to 10 10 to to to 4
/o to to 10 '0 <<0 '0 tD 5
to <<0 10 to 10 10 10 to 6
/o ID 10 '0 to to to 10 7
/o to '0 /o /O /o to /o A = Pan weight (mg)
Tray color code:: f0 D*j Analyst: TS 15.11 \5M R5I m.w I3.S? 15.51 aw l<<U8 Date: 0$. d&-)%
B = Pan + Larvae weight/mg)
Analyst: \f v\M **un 21.1& x"&.oS 2<<.VL -tj.*\ 7S.U w/>>&
Date: U-rV< S C = Larvae weight (mg) = B - A Hand calculated. << W l <\.<r*> i^n &.*S 8.MO g.->>g ^.w e.nS Analyst: JV Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculated. .
& (^
Analyst: M 0* 0' 0* o' 0" 0' f}\ U J^4 Average weight per Percent reduction initial number of from control (%) o.&H O.H7. O.a^o larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w - wounded.
Comments:
SOP AT20 - Exhibit AT20.3, revision 11-01 -14 Page 30 of 91
TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16,2018 Pimephales promelas Chronic Whole Efflucnl Toxicity Test EPA-82l-R-l)2-013, Method 1000.0 QuMllty Control Verification c<<r Diita Entry, Calculations, and Siuiixiienl Aniilvse<.
to Environmental Testing Solutions, Inc. Project uumuen 13039 I.'MI l.l! ' '.jll'l i i'l ' ' '?*.'*. -*i'.';ll 1. !,l:i:,l 1 . *;'.*'..:' ?." '
Cnnaitireftm^Q K:tll,.,l, l..iit..l (tgmtm-.f ni...Uun.l.ir..M.in.., II -l'..n . Urn, I mM ttclfffcl Iri.nl \5VIulil I.Hnnl.ir.;; U'.ijlit 1 Inllijl (t(.ntl.rr .* *- Mi>n ",i."it 1 L.-rlIlrirnliil bifm? 1:,H;I ...I.^lillni,?! 11-,\ IIUI..I,,, .,1 I..C (.,* -I two Inillji lUflWbrii-f S.lfl lli 1: t'.ilill ....*!
';:;,'-':"V'"
cc 11 Ill 13 :'*: 2l .p II 40 0.840 0 840 C<< !. on 10 10 i :* .1" :-i >7 S7S :! S;S 0 S7K a 4Q 0.880 5 7 Mlll.ll QJS89 5.: Nfll :i[iEllil'.tltI.
Non-1 railed BE 10 III 1! 62 (3 t 1! ?l.??l u'*'*;**>
i i
id 1 I 23 23 98 S 75 0.S75 HS?f
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\ 10 10 1? 77 Tl *?.) 9 27 ?!?07 0 >>2"
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O III IS l.i 22 25.03 '151 0.9SI t>>.'->>m P III 10 15 54 1 . *: B 66 " 866 a sou O 111 l'j I.i IA 15 5 72 0 S77. 0.872 u 10 11? Ml SO 23 91 9 1)5 n 905 .>>!*:--
ISA* O'li? MI*U' tV9H *J," -2.7
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^ iq 10 II T. 23.81 9,09 ,l.!ii,i 1; .t;;f, hiii",;. B8SS .1: 100.0 tUMS 9*2 IIJ w Ml 10 15 '-*'* 24 37 9 13 0.313 o*ji i
\ in II' 15 7S .'.'* 10 9 57 0.053 u 1'52 V Id 10 15 l(j 24.91 *177 15,973 0972 t 10 10 1! 64 24.67 '.1)1 M '*!;* 1190' I1KVK Intake 6£84 Mlil.'l i.i.K.sj 7.8 lU
\.\ Ml 10 Ml SI 22 ' :* ?. -17 0.847 0,641 mi 10 Hi l 1490 ?) US .: 15 0815 atits nmr.-iU I'M; MSD MminiLi-iiiSl^iiflcatrt SJiltei<ii.-..'
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l*,MSH: PMSU .* a mtasuraoftcsi pru&sion rtwffittSD is ilwran mini i'Cvcon! ciilTortiii:*; iv.-tivcon the uwhh! ..it.! iii.-.liiii,;qr ih;ti t,;.i lv <<i..'.:l irvi.i:-r;*fs-trc^Ilv !.ii.!iiili:.!iii m :* 'vh;>icdiSucm nwiaiv t--i lul.ilir:
Drninrlt's MSI) wiluc; 1-uim.v I'MSDlioumlitcwiimiial liv I'Sfii'A nuiii p^cvim^t 12%
I'MSl) UpparPNtSObcttwddtH^rmklttd \-, USEPA (fQJft pciswltilo} HW LmvL'f .irnlvipjivr j'MSD bounds were (!cK'tmtin;i! Norn Hi-' t'iili iukH0j& petecmfta, hsjicclively. ofPMJiO .Inl.ifioifi i:PA'? WUT (nlwrltiUeratisy V.miliiliiy Study (USItPA, iiKJI.i. US!:!'A lw\hi I mi-'- main toot it Klnal Rtrpthi Inhstibo .c. \..i.,ii'..ltiy si.,uwi i-i,.\ si.^t-t nClwqnu: amiAeweWhttkEfllitaitH faKMHQ r<<<< ^fcthwtft, V'oft>>uei 18nd3rAp]i<<ari<< iiPArffai-B-Oi-OOJ and EPA-ttai-S-QI-iX)
I Ijj tiirviftwnwita) ProaMUottAg<<a^, rifis.-iTin.iii. oil Jrftww,
TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16,2018 H^3 lW¥f^
Statistical Analyses J Cnvlronmfnul'fimifigSoJuUwKtnc.
Larval Fish Growth and Survival Tost-7 Day Growth Start Date- 10/9/2018 Test ID' PpFRCR Sample ID: TVA / SON 101 End Date 10/16/2018 Lab ID ETS-Envir Testing Sol. Sample Type. DMR-Discharge Monitoring Report Sample Date: October 2018 Protocol: FWCHR-6PA-821-R-Q2-013 Test Species; PP-Ptmephales promelas Comments: UV Treated Conc-%
Non-Control 0.8400 08780 09490 0.8760 UV-COntrol 0.8410 0.8610 0.9030 0.9410 10 7 0.7490 0.8760 0 9360 08900 214 0,8740 0 8780 08960 0 8190 42.8 0,8780 0,9270 0,9810 0,8660 85.6 08720 0,9050 0.8940 09750 100 0 7660 0 9090 0.9130 0.9520 intake 09720 0.9030 0.8470 0.8150 Transform i Untransformod 1-Tailed fsot<>nlc Conc-% Moan N-Moan Moan Min Max cv% N t-Stat Critical MSD Moan N-Mean Non-Control 08855 0.9976 0.8855 0.8400 0.9490 5.162 4 UV-COntrol 0.8876 1.0000 0 8878 0.8410 0.9410 5 099 4
08884 1.0000 107 08628 0.9718 08628 0.7490 0.9360 9.278 4 0 598 2410 0.1007 0.8884 10000 21.4 0,8668 0.9763 0.8668 0.8190 0.8960 3.835 4 0.502 2.410 0 1007 0 8884 1.0000 42.8 0.9130 1.0284 0.9130 0.8660 0.9810 5.745 4 -0 604 2.410 0.1007 0.8884 1.0000 856 0.9115 1.0268 0 9115 0.8720 0.9750 4.882 4 -0 568 2.410 0 1007 0.8864 lijooo 100 0.8850 0.9969 0 8850 0.7660 0.9520 9.228 4 0.066 2.410 0.1007 0.8S50 0.9962 Intake 0.8843 0.9961 08843 0.8150 0.9720 7.790 4 Auxiliary Tosts Statistic Critical Skow Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93304 0.884 -06932 0.22375 Btertlett's Test indicates equal variances fp = 0.66) 3.2891 15.0863 The control means are not significantly different (p >> 0 95) 006995 244691 Hypothesis Tost (1-tail, 0.05} NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetfs Test 100 >100 1 0.10073 0 11347 0.00182 0.00349 0.75724 5, 18 Treatments vs UV-COntrol Unaar Interpolation (200 Rosamplos)
Point % SD 96% CL(Exp) Skow tcos MOO IC10 MOO IC15 >100 (C20 >100 IC25 MOO IC40 >100 IC50 MOO 50 100 Do-so %
Doso-Rosponso Plot 1-taii. 0.05 level of significance fr Page 32 of 91
TVA / Sequoyah Nuclear Plant, Outfall 101 Intake October 09-16,2018 Statistical Analyses Environmental Testing SotutlonK Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 10/9/2018 Test ID: PpFRCR Sample ID: TVA/SON 101 -Intake End Date: 10/16/2018 Lab ID: ETS-Envir. Testing Sol Sample Type: DMR-Discharge Monitoring Report Sample Date: October 2018 Protocol: FWCHR-EPA-821 -R-02-013 Test Species- PP-Pimephales promelas Comments: UV Treated Conc-%
Non-Control 0.8400 0.8780 0 9490 0.8750 UV-COntrol 08410 0.8610 0.9080 0.9410 10.7 0.7490 0.8760 0.9360 0.8900 21,4 0.8740 0.8780 0.8960 0.8190 42.8 0.8780 0.9270 0.9810 0.8660 85.6 0.8720 0.9050 0.8940 0.9750 100 0.7660 0.9090 0.9130 0.9520 Intake 0.9720 0.9030 0.8470 0.8150 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Non-Control 0.8855 0.9975 0.8855 0.8400 0.9490 5.162 4 UV-COntrol 0.8878 1 0000 0.8878 0.8410 0.9410 5.099 4 10.7 0.8628 0.9718 0.8628 0.7490 0.9360 9.278 4 21.4 0.8668 0.9763 0.8668 0.8190 0.8980 3.835 4 42.8 0.9130 1.0284 0.9130 0.8660 0.9810 5.745 4 85.6 0.9115 1.0268 0.9115 0.8720 0.9750 4.882 4 100 0.8850 0.9969 0.8850 0.7660 0.9520 9.228 4 Intake 0.8843 0.9961 0.8843 0.8150 0.9720 7.790 4 0.085 1.943 0.0801 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0 01) 0.95112 0.749 0.40793 -0.9433 F-Test indicates equal variances (p = 0.51) 2.31582 47.4683 The control means are not significantly different (p ~ 0.95) 0.06995 2.44691 Hypothesis Test (1 -tail, 0.05) MSDu MSDp MSB MSB F-Prob df Homoscedastic t Test indicates no significant differences 0.08008 0.09021 2.5E-05 0.0034 0.93508 1,6 Treatments vs UV-COntrol Dose-Response Plot 1.2 1-tatl, 0.05 level of significance Page 33 of 91
I r>3 ET^O fp
>£ Page 5 of7 Species: Pimephales promelas Date: 1Q-Q9-18 Client: TVA / Sequoyah Nuclear Plant Outfall 101, UV-treated Daify Chemistry:
Day (Analyst identified for each day, performed pH, P.O. and conductivity measurements only.)
1 Analyst ML HJ j£_ .23^ TS/*J jLa Concentration Parameter ti&t&li&S&Zli* ,te:i^-I<<.<.sJ*l ^Sifig^S/5!
pH (S.U.) ^yt JSL ^jlg? 7*ff0 7.9/ 32:
DO (mg/L)
Conductivity
~?>>? 7.6, Tfr
^*tf
-3g-
"PIS:
^i_ 3c=d.
CONTROL (ujuhos/cm) Zo& a'-.-;^^ ^ *2?5
AlkaJinity UV-treated (mgCaCOs/L) S<\ :-.i*SJl'l ->',: w££^}ii. S^
llnrdness F7/?'-r'^51T^~
(mgCaCCVL) &T- SO
^Temperature (°C) -M\n >sy>> -vrt-1 TAJL -v\.S pH (S.U.) ttH 7.<W -?.?& 7.H? 776 ^17 DO (mg/L) &o ~7.4 &o_ 7.£ g.V 7M.
10.7% Conductivity (umhos/cm) mu r&t£r,*&?45 3H 30/
^Temperature (°C) -A-* ^cl3^ -\A-S TsAA -\i\-W pH (S.U.) %*3 J^&g_ 7.>>g> 7^* 2kL2-.
^
~Z77 DO (mg/L) &L -?.? g.i &. jg£_ Iffl 21.4% Conductivity (umhos/cm) 2(olr ^t^,.,; 2^1 :V ..?<<" /".**'**H #?
^Temperature (°C) *x*>8> 1H-W -l4>1 nA-2>
Jdi Vl*
pH (S.U.) 3£L 7<<4.g 3&L 2M_ XK_ 3£E DO (mg/L) .AL J32_ AZ .3£_ gz ^>>>
42.8% Conductivity (umhos/cm) 23^ ISO 334
^Temperature (°C) JHiL -lil-U "\s.o vu>> "kkx *vM>>
pH (S.U,) 'H+taH ?^.2 7.go im. 7.66" %\7r DO (mg/L) <£i. _^tf_ #2 _^3_ g.? sQPr 85.6% Conductivity
(^mhos/cm) /&<? /?1 /^
^Temperature (°C)
^ -vH-S is.o TA-V, "WTt TH--L pH(S.U.) 7*61 7.S*T 2M. m ^3 DO (mg/L) &]_ j£*_ ^3 7-<? *2_ u?,^
Conductivity <<f> t./v*l (umhos/cm) /V2 /^7 /37 :-i^:-r5rdi/^"
^Alkalinity 100%
(mgCaCQt/L) A SO c^lfSj';
Hardness (mgCaCOs/L) sA i^'.?^4; ST.
TR chlorine (mg/L) ' ^'s^y^c
<Q.IO <D.I0
^Temperature (°C) -is.t> TH-1 TS-t> iM-b "TfiTJ" "TX^"
pH (S.U.) 3^2_ 7-55 7.tfo -Z36. jo^a.
DO (mg/L)
Conductivity
^JL 7-B ff.3 Ro
tol-a?;^lp (umhos/cm) /<<< /Sg /35 100% ^Alkalinity (mgCaCQs/L) Sv<< W tV*: ST-Intake
^Hardness A*'^l^4 "^ %J ? 7^ 1?r^5'v *"'*
(mgCaCOj/L) S& 51. 'S^flH
^-c->^t,<<r-~r- >r^
TR chlorine (mg/L) <Q.'Q <QJO '^^^^^rflS,
^Temperature (°C) T£.Q "vCT "IS. I 141 "K-0 q^b Initial I Final Initial Finn! Initial Final
^Temperatures performed at Ute lime of test initiation,renewal or tenninationby the analyst identified in the Daily Renewal Information tablelocatedon Page 1.
ilinity, Ijardncss and total residual chlorine pcrfonncd by theanalyst identified onthebench sheet specific for each analysis and transcribed to thisbench sheet
£ Total residualchlorine was performed on non-treatedOutfall 101 and Intake samples.
SOP AT20 - Exhibit AT20.3, revision 11-0I-14 Page 34 of 91
IFF1 ? ? i':~.
><<- Page 6 of 7 Species: Pimephales promelas Client: TVA/Seauovah Nuclear Plant, Outfall 101, UV-treated Date: 10-09-18 Day (Analyst identified foreach day, performed pH, D.O. and conductivitymeasurements only.)
3 4 5 6 Analyst l5f&0 ft?t- T3 TS HS /<T TS.
W^8 Parameter teifil Concen
'.'* !-v-"^-"-' ;-'4v';t^-,!.*i tration
_.^
pH (S.U.) T-.13 ^-.Wl "7.HA 7.70 ^7.26 "7."^ -7.IS-DO (mg/L) -Xfl *3.1 <S.o t?.l 9.0 "7.6 ^-<<? y.o Conductivity
";;':. .>">>T'": /;--.: -.- ^ :
CONTROL (umhos/cm) 2o\ ^^5" 3/? 31?
^4 VK Alkalinity ','- -^^^,'"":' "* \ ,;'
UV-treated (mgCaCOs/L) tl-^d^j S<\ fcv'-v>.:.
ira.
A
Hardncss * <<S. ;]yr'^ '"*'*: '** .*.', .'S-rvj *>>i T ",'" \t *
(mgCaCCh/L) So ** f '.' *" _.T
^Temperature (°C) *bt. i -o\.u -lA--\ "Ll.S *\A. f AK.fe -\HA *m\A pH(S.U.) *%ks<\ *>.o^ *>.uo "7.37 767 ^7.2ff 1*8 7/H DO (mg/L) W ^3 S.i fcl £.0 U? T.^ s-.i 10.7% Conductivity (umhos/cm) m 5}*80
/ (,*'.' "* 7 * *,* ";
W "3oo ,^'.'i:;:v?;;/;; _;
^Temperature (°C) -u\A -v\.s -\A-C *V\.-S -v>\.-4 -te\M -tin XH.4 pH(S.U.) A.i/? T-.cv T-.S* 7.39- 7.&M 7-27 T&//? ~7.0?
DO (mg/L) *XQ 1~n. <<.\ &/ 9.1 7.5 ^.0 ?/
i:,->li^
2L4% Conductivity (umhos/cm) %fr 5UH- <</' > "-/v 1 3L*\ L *..".* .^'^.'ir*. ^T4/
Tempernture(°C) -blA -V\-S nA-t?- TA-i -v\.i TA.H Til.-l -Ll.S pH (S.U.) a.y0 ~*.°le ^.ss- 7.37 7.61 7.Z5" v.^e -J.O~7 DO (mg/L) 0.0 1JK ***! }?.-? 9.1 7-5 Ao S3 42.8% Conductivity . *;,:r~ ; ' l (umhos/cm) 344 331 W -ZH2 "rQc/'i'Cl't'*'..
^Temperature (°C) -\il-<\ vi-s V\-fc -vV-i "bA-Sr -UVS "fclf 1M.3.
pH(S.U.) "^'A T-.n ^r.*H 735" *7.5-6 7.Z7 7<*, U97 DO (mg/L) 0x> T-n- <i.\ R3 J?. 5 *?.p. <<l V.3 85.6% Conductivity (umhos/cm) V\\ Half /7g /67 kM;\:S
Temperaturc (°C) IS-O -\M Vl-6 ">>a-i vV-4 1>t--I tA.* m.<>>
pH (S.U.) <\lf\ "^.Ol* T--40 757 7.53 7./9 T.'W &9<2>
DO (mg/L) Q>.\ ^M> <&.* >>.a t?.a ^7.e A. <?.2 Conductivity (umhos/cm) \A% ; /-jf^lff im* *.' \ , ,V > '
ItoO ./IvSvEi^ /5-S "Alkalinity \ ' ' \
100%
(mgCaCCfc/L) *I'V '.'"i-'-ii *;<.'.>>' sA l.-i .-*' ~'2~~ '*'* . :-l."3iC£:4?z ' *'- .'.'*% *..
~?*~,7&]~ "^ r'7<>f?."*'"/ ^^' ^;"j Hardness (mgCaCCfc/L) s^ J.' **)" .' r-"-,-' i -
TR chlorine (mg/L) ^?5.:T;r^l' :3^&YV <0.lO !-* 1 *WTT?* *" ".'
7v"r" '.*-'fe^S^ J=i* u;:.7":
Temperature(°C) -is-o ^K.X. "tH-l X<<\^l- -V4-S TH-S ^4.1 !<<(.<<?
pH (S.U.) /v^1 UPiUf 1.H-3 V.cKff 7.^*? ^7./? ^.^ U9D DO (mg/L) 6.2. '4*1? <<S.o S'.S >>.X ^?.9 &i 81^
Conductivity (umhos/cm) MZ KSO I3S / ,v^ *,t-5 /^? 1HZ 100% ^Alkalinity ST-
^;:""'; yt' **v\vrv,t*r Intake (mgCaCOs/L)
^"rf:'!;-
-J 'T "**T 4
'"*'*.-'**'I:
Ilardncss * "' '>'£**? - **
(mgCaC03/L) s4 ^ ' *" <>V"- "*-* '*~?* S.^ V^* ' "' -40.10 1 -,, v ->>- --
f.n^g'SS ^i;<<;"?"'ij 1 ^Temperature (°C) T<i.V tA-S ISO -v^M xH.-l \4-n *HI Vife Initial Final l Initial Finn) Initial Final Initial Final l inperatures performed at tlie time of test initiation, renewalor terminationby the analystidentified in the Daily Renewal Information tablelocatedon Page I.
inity, hardness and total residual chlorine performed by the analystidentified on the benchsheetspecific foreach analysisand transcribed to this benchsheet
\A . Total residual chlorine was pcrfonncd on non-treated Outfall 101 and Intake samples.
SOP AT20 - Exhibit AT20.3, revision 11-01-14 Page 35 of 91
T fr!
Page 7 of7 Species: Pimephales promelas lJO,>>Tflj&dVrfc Client: TVA /Sequoyah Nuclear Plant, Outfall 101,<<V'ti'cakd ^^ Date: 10-09-18 Daily Chemistry:
Day (Analyst identified for each day, performed pH, P.O. and conductivity measurements onlvJ 0 1 2 Analyst HJ MS HJ T5 *<<9*V yu Concentration Parameter 'SZgfflShZJA *SB?r;r£i;i fr<<Coi-.':rzi3 *.***>>>>>. ',, >>-* >'
pH (S.U.) *ls.V 7h*d 7-^s- 7.62 n.s^ %l^
Control DO (mg/L) 7.* 7.ff -7.i>> ^-<=> P.D (aia Non-treated Conductivity (umhos/cm) 3/9 lffij:*"*~?& !:"~* u? ?i6 kJF:^iL-^:T?L Boa ?.^fD;-.t>>/V;
^Alkalinity /?5^^"'X^'VV; (mgCaCOj/L) ^0 **.**; -r:V',V
\* s'?
"V ^S£::.-:
^Hardness
' >'r-:' '*':.^*^
'. '" !> "~* .'"**"
(mgCaCOj/L) & <£0 "Temperature (°C) l m\.\>> *cM -lii.n ik\A *v-UW -e\-5 l Initial H Final l Initial Final l Initial Final l Day (Analyst identified for each day,performed pH, DO. and conductivity measurements only.)
3 4 5 6 Analyst \A^ 8s<- -&3fc It>>.i5.v T5 TS ;W-f MP TS Concen tration Parameter r^V-V ** *.; \s>-?:'-V.- *}
ItM^f £?£'.*'- rV ]l£l:-l'*£ i§i^J§^r~-'
pH (S.U.) Q.ul 3.lo *vso -739 1.-70 7.Z0 7>yq 7-0&
Control DO (mg/L) ^> ^5 -\.fi 79 7.7 7.? 7^7 *\A Non-treated Conductivity 3GD tv,c^,;\ ,.** I'/ **'_. v 313 >'-**!!i *"}'*'*r (umhos/cm) 3£?
Alkalinity (mgCaCOi/L) ^V, tv^i <<v"!'1' sg
, ' f . r.;" >'*
^N^ \t* ,*-;*?:'""
\ 'A V.;**V~'i/ Ntf^l ,'i~r ./"\ ~:
'SS^i *.^-'!'^ **\
Hardncss (mgCaCOj/L) <£0
^Temperature (°C) -4A -u\.S v^w ->>*.<, via x^M -vin *rtA Initial Final l Initial Final Initial Final l Initial Final l Temperatures performed at thetimeof testinitiation, renewal ortennination by theanalyst identified in tlieDaily Renewal Information table located on Page 1.
Alkalinity hardness andtotal residual chlorine performed by the analyst identified on the bench sheetspecific for eachanalysis andtranscribed to this bench sheet by: ^_-
SOP AT20 - Exhibit AT20.3, revision l l-Ol-M Page 36 of 91
TVA /Sequoyah Nuclear Plant, Outfall 101, UV-treated October 09-16, 2018 T Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-82I-R-02-0I3, Method 1000.0 Daily Chemical Analyses Environment] Ibttlng Solution*, inc.
ProjCCl nijti'.lirt: 13633 Concentration Parameter l)n\ O 1>:iv 1 Day 2 Day J Daj 4 Day 5 Day 6 Initial Final Initial Phial tnlttal Pinal Initial 1 illlfl initial Final Initial Pinal Initial Final lil MNi:) 7 CO 7 70 7.75 7 52 7 50 7 17 7 61 7 10 7 5n "* ;<i 7.70 7.28 7.40 7 06 DO (mg/L) 7.7 78 7,8 19 so 6.6 7.7 7 5 7 s 7 0 7.7 77 7 7 7 0 Control, Conductivity {untbos/cm]l 519 316 302 50(1 207 313 313 Noo-lrcntcil ?\ll(;iliniiy [mg/L CaCOJ 60 50 58 Hardness [mg/L CnCOj) S4 XO 30 U'iiilicr:iiui'i' ("C"l 24 6 24 4 74.7 24 4 34 ?? 24 3 24 7 24 5 24 ?* 24 5 24 7 24.4 74 7 24.3 I.1KSIU 7 70 7 71 7 S7 7 50 7 SI 7 15 ? 69 7.13 7<J! . 42 7 70 ** 7.46 7 IS DO(taeM 7 0 7 7, 7.0 7.8 XI 7 1 7 X s 1 g.0 X 1 x.o 7 0 7 0 8 0 Controti Conductivityfumhos/cm) 305 311 205 300 205 ;ix J 17 UV-treaCcd Alknltnit) (mg/L CaCOj) 59 JO 5" Hardness(tngCL CnCOj) S2 so XII TTeatpefStttre ("Cl 2-1.7 24.6 24 X 24 7 24 7 24.5 24.X 24 0 24 7 24.5 24.8 24. g 24,7 24 4 pll(SC) 7,74 7 60 7.83 7 4;-: : 7', 7.11 7 60 7 60 7 60 7.37 740 7.25 7 48 7 14 IKHinti/l.s 8 1! 7.6 10 7.5 X 1 7.0 7?/ ? s S.I SI so 7 7 7 0 S.I 10.7% 292 Conductivity (tunhos/crn) 77o 4i4 501 280 298 300 1cmncraittFC ('Cl 24.8 34 5 24 0 24 5 3.4.7 24 n 24 0 24 5 24 X 24 5 24.S 24 4 : 17 24 4 Dlt(SU) 7.73 ; ? .: 7 so 7 45 7 77 7 12 7.60 7 06 * '*? 7.38 7.64 7 27 7 IS 7 00 DOOM",'!.) S.I "T "J B 1 78 X3 6.8 7 0 - ; X 1 X.I X ! SO :-;.:
2 1.4%
ClitKlUf tivitV (lIImIk>s/CI111 267 201 269 7 77 204 381 YIA l\'ii:[K'r.i!ufv? f "Cl 24. 8 24 6 24.0 24 5 24 7 24 3 24 9 24 5 24.X :; ' 2.4 S 24 4 24 7 24,5 pIKSU) 7 71 7.65 7 84 7 48 7 72 " 12 7 68 7.00 7 55 7.37 * *: 7 35 7 48 7.07 HOlme/I.) S.I 7 7 ,5.2 7.8 X2 6.7 so 7 X 8,1 x 7 s 1 78 S 0 X 7 42.8%
Conductivity (tunhas/cm) 234 .750 234 244 231 !4E 24 7 Tt'mitcnitnrt' CO 24 S 2-i.O 25.0 24.!> 24,7 24 6 24 0 24 5 24,8 24 5 24 $ 24 5 24 S 24.3 ilLK.St!) 764 7iO 7 SO 7.44 7 65 7.12 1 64 7 II 7,49 7,35 7 56 7 27 7 40 6.07 DO(mft/L) S.I 7 8 *5 2 : o S3 6.7 SO 7 7 S.I X 2 X2 7 8 X 1 S.2 ns.fr/,.
Conductivity (pmiios/cm) us 179 [62 171 166 I7S 167 Temperature ( ( 1 24.0 34 5 25 0 74 (I 24 7 24 2 25 0 24.4 24.S 24
24 8 24 7 24 8 24 6 iiHtsSi 7 61 7 59 7 78 7 4?1 7 60 7 (? 1 706 7.46 7.27 7 53 7 18 7 14 6 96 l)()(m>>/L) 8 1 : s si 3 ,' *j 5 5 6 X S ! 7h S.I 8 2 8 ' 7 S 8 1 3.2 CmttliK-livitv (umhos/t'iul 142 157 157 145 146 160 155 100% Alltalmiiy (m<</CCaCO,) 54 Si? 54 Hardness (niK/LCsiO).,) 54 52 54
1"t?il Residual Chlorine (mc/L '0. 10 0 10 :() .10 l emncratorc ((') 35.0 74 7 25.0 34 6 24 S 24.5 25.0 24 2 24 O 24 2 51,8 24 1 24 0 24 5 n'Hfsui 7 S3 7 53 7X0 7 56 7 54 6 00 7.61 6 06 7 43 7 28 I 40 7 13 7.45 6532 pOung/L] ?*; i 7 8 8.3 8.0 8.4 0 7 82 7 0 SO S3 Q 7 7.0 8 1 82 Cotnhicfivhv (jiiuhos/cml 142 158 135 14 2 155 I4X 142 100% Intake AJknlinilj (mg/L CaCOj) 56 52 52 Hardness lm<<<l CnCO.,) 58 52 54
" I <?l:il Ki'Mdnal Chlorine (miOl, 0,10 n in
0 10 Temiterutnrc ("CO 25.0 24 7 25 l 24 3 >> 0 24 0 25. 1 24.5 25 0 M 4 24 7 !4 7 24 8 34 V
' Ncrc. Vital resldu 1chlorine was performed on non-1 eattd Ouilhl 101 ami Oil ike samples File: sqn10i_10Q918chem Entered by T Steeper Reviewed by v\.
ilSf, K.
Page I of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia Client: Tennessee Valley Authority County: Hamilton Facility: Sequoyah Nuclear Plant Outfall: 101 NPDES #: TN00264S0 Project #: ftfcgfo Dilutionpreparation information: Comments:
Dilution prep(%) 10.7 21.4 42.8 85.6 100 Effluentvolume (mL) 267.5 535 1070 2140 2500 Diluent volume (mL) 2232.5 1965 1430 360 0 Totalvolume (mL) 2500 2500 2500 2500 2500 Test organism source information: Test information:
Organism age: <24-hoursold Randomizing template color /?&AW6 Date and times organisms were bom lO'OVlf" OW*> *ro 0<V%S between: incubator number and shelf Culture board: !O>>01<<l.i A ^o-n-iEft location: T.C5 Replicate number:
Culture board cup number: YWT batch:
3b M3 UK H lo \%m tO-0H<£ Transfer
- ^ ~ vessel
^ information
. . pH<< ,"\..&SS>>a Temperature" iAA°C y,, x.
Average transfer volume(mL): j <Q,25mL Seietmstnmt batch:
O-0Wf Daily renewal information:
Day Date Test initiation and feeding, i MHSW Sample numbers used Analyst renewal and feeding, or l batch used Outfall 101 Intake termination time 10-09-18 IDIO 10-CVU \ iJPOg.Ql \&(00$*<&
10 IS 0^03 4-IQ-OV^ /fiAOcf.cn-10-11-18 yg^o^^ -4 10-12-18 oW^ io>>tn-\i *frioio>>M iCiofo-M-ii o<^S UlDl0ri\
iQ-ovtg 1&ICH0-&L 10-13-18 o*\sfr \U0llJtt* UlQVl.T*!
10-14-18 KMMfiA
A o<V^ lildkliA. VClfUl.lJ, \*ioa>>x*> A 10-15-18
Q<<\H&r\
10-16-18 6<\\\ uum/tt l&lPtl ^ 4t il (WUb v. "?£* v :*\ ^v* *
^ l&:&\
Control information: Summary oftest endpoints:
Control-! ControI-2 Acceptance criteria
% of Male Adults: 01. 07. £20% 7-day LC50
% Adults having 3°* Broods: > 1QQ7, JO&7 >>007. £ 60% surviving adults NOEC
% Mortality: 1067*
07. 07. 520% LOEC Mean Offspring/Female: > *OQ7.
3o-H T.Cy.1. £ 15.0orTspring/female ChV >*OQ7.
% CV: 8.a7. S-S7. <42.0% IC25 >tOD7.
SOP ATI l - Exhibit ATI i .2T revision 11-01-14 Page 38 of 91
immsm
,,' t<<*n>R>><<ta*ITti<<(vj$rfmlcnVin Page2 of 7 Species: Ceriodaphma dubia Client: TVA / Scquovah Nuclear Plant, Outfall 101 Date: 10-09-18 CONTROL-1 Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 Young produced 0 0 l 0 D 0 O 0 o 0 O Adult mortality v_ V \_ \^_ \~ V_ \ ^ \_ \_
Young produced o o 2
<>. h r\ o 0 0 n o Adult mortality \_ \^ \- U- \_ ^_ v_ c_
3 Young produced O Cb a o o o 0 0 O o Adult mortality VU \__ \ ^_ \ v__ v_ x 4 Young produced <A <<-i 4 5 *5>> <>> ~~>0 >3 Adult mortality v_ v_ v_ v_ V <>_ \_ \ \_ >><_~~
~~Yonng produced~~
~~o o Adult mortality U~~
~~v_ \_ U. \__ U \_ V- >^ l_~~
7 Young produced iA >>4 in v2i >>1_ t<>4 i$ * & iS Total young produced 7.1 s\ 31-Vk 2>\ 34 T-g XT "bt- *Si Finnl Adult Mortality v_ v_ \ v_ \ \__ V_ v_ \ \
~~X for 3rd Broods , 2S, Y~ ?<- ^ ?fr >C >^ >c ?<- 7^~~
~~Matt; Adult *nortafity it = live, D~~
~~dead).JJB<< spfitbro<yd(single bro 3dsplitbetwe entwo days), C6 ** carry over(GflVprins> carried overwlJiadult during transfer)~~
Concentration:
% Mortality: 07.
Mean Offspring/Female: 2)0.4 CONC: 10.7% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 Young produced 0 I
ft 0 0 0 0 0 O o O Adult mortality v^ \_- V_ v^. u. v_ v_ \- >^. c_
Young produced 2
O o o 0 n O o o o 0 Adult mortality v_ v_ L_ v_ \^ v_ V l_ L_ L.
3 Young produced e> 0 0 o 0 O 0 0 c b Adult mortality u. u_ v__ ^ \ V_ *w ^_ l_ L_
4 Young produced 4 s M M s 4 ^>> s s S Adult mortality u \^_ \_ v_ v__ v_ v^ v^ v^.
5 Young produced IT- '^ *^ >( 'D i^S l?i <<o IT- >T_
Adult mortality V_ V v__ \^ u_ L_ t^. \__ V_ V-Young produced O
<j O 0 O 6 O 0 0 0 O Adult mortality . \_ v_ v_ L_ \ u v_ \_ L^

~~Young produced l\o l~~

Total you ng produced 3ft 2>1_ ^2>> 3S 3*. 3b ^>n iW 3>>fe 3S Final Adult Mortality v^. V_ v_ v_ \ v_ l_ L_ .U V-A'ow: AdultmortalUy (L = five.D >> dead),SB=split brood(>>nsJe broodspfitbct\>,cen hvo da)*s>. CO - carryover (offspringcarried over withadultduringtransfer) Concentration: % Mortality: 07. Mean Offspring/Female: M.\ % Reduction From Control-1: -tt. X 7. SOP ATI I - Exhibit ATI 1.2, revision 1l-Oi-14 Page 39 of 91 lPp-j csp3 rzzj i:~ ! . tj<<wiHvn>>itufTtisJisgSefcMk>>ilnc Page 3 of? Species: Ceriodtwhnia dubia Client: TVA / Sequoyah Nuclear Plant Outfall 101 Date: 10-09-18 conc: 21.4% Survival and Reproduction Data Replicate number Day 10 Young produced _Q_ O _£l Jl .a sl SL Adult mortality v_ Young produced .Cl -Cl _D_ _0_ JL £L £L Adult mortality U v^ Young produced _Q _Q O SL Sl o O o O Adult mortality Young produced H +/-L <A ^ +/-L Adult mortality Young produced lb l"l_ \i_ il i^S '^ t o Adult mortality V_ Young produced Sl O o o Adult mortality v_ v_ Young produced 11 \& ig 7.0 -to A 14 X\ t*\ Total young produced ^2> v* h°\ *s iS 3M 3\o 3>>S 2>>1 32> Finn! Adult Mortality ^ Ate Adiilt rooitafiiy (L* live, D* dead). SB*>> split brood (angle btood spUt bemtseq hvo days). CO
carry over (otBpring carried over with adult during transfer).

Concentration: % Mortality: 07. Mean OfTspring/Fetnaie: 3S.5 % Reduction from Control-1: - It. \T, CONC: 42.8% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 s 9 10 Young produced 1 r> O O 0 0 & 0 o o o Adult mortality u v_ \^ L_ L. l_ l_ u U L_ 2 Young produced 0 o C) 0 0 C\ 0 ft O Q Adult mortality i_ l_ L_ \_ v_ v_ L_ l_ U 3 Young produced £> O o o o 0 O a 0 o Adult mortality v_ v__ v_- v_ U. V \- u~ V. 4 Young produced 4 4 \* v* 4 4 ^> s u s Adult mortality v_ \_ v__ v_ v_ v_ \_ u. v_ v_ 5 Young produced i4 i4 IV \T_ ^ <<-b >>?> u >>3> ii Adult mortality v^ s_ \_.

~~V ^ ^_ \ ^_ <^-~~

6 Young produced rs 0 O 6 D O 0 O 0 o Adult mortality ^ \_ L_ \-_ L_ u_ \_ ^. ^ Young produced 7 n n T-\ xo n "r- t\ x<\ "Li -LG Total young produced 3S 2>i 4t> 2rt *\ 3\ *<\ 3S Mt> 3t Final Adult Mortality V- \ \^_ v_ v_ v_ v^_ 1_

~~~ Vw_~~

AVwe: Adult mortality (L >>live, D>>dead), SB <<split brood (single brood split betweentwo days). CO <<canyovwjojggrm^ Concentration: % Mortality: 07. Mean OfTspring/Fernale: ilo.S % Reduction from Control-1: -"io.\7. SOP ATI I -Exhibit AT! 1.2, revision l l-Ol-M Page 40 of 91 3 It t>2

~~i IrrHiaMtHflUfTcTttr^SctuttaA&tec.~~

Page 4 of? Species: Ceriodaphnia dubia Client: TVA / Sequoyah Nuclear Plant, Outfall 101 Date: 10-09-18 conc: 85.6% Survival and Reproduction Data Replicate number Day 8 10 Young produced O XL O H o SL O Adult mortality ^ Young produced G D_ SL 0 SL SL Adult mortality L_- u. u. Young produced O D o O O _0_ O Adult mortality Young produced 4 Adult mortality Young produced v4 <<4 l*t_ *"b \x. *:b \b j>L IT \A Adult mortality v_ Young produced O O Adult mortality Young produced \& rt T-T. \i Tl V\ TO TL TO \<\ Total young produced 2>S 31= m 3-V Md 34 art ^\ *1 ** Final Adult Mortality Note: Adultmortality (L

~~live.D << dead), SB - splitbrood(single broodsplitbetween two days). CO* carry over(otTspring carried overwith adultduring, transfer).~~

Concentration: % Mortality: Mean Offspring/Female: % Reduction from Control-1: -iyn. CONC: 100% Young produced 2 n 0 o O O ^ 4 Young produced Adult mortality 6 Young produced 7 Young produced Final Adult Mortality Concentration: % Mortality: Ivlean OfTspring/Femaie: Vo Reduction from Control-1: SOP Page 5 of7 Species: Young produced £ Q. Adult mortality Young produced Adult mortality Young produced J^L Adult mortality Young produced XX Young produced Total young produced Ho 2*o TTt 2>>\ X*. A'ote Adult roortaBly 07. 2n.< Survival and Reproduction Data Replicate number Day I 2 3 4 5 6 7 8 9 10 1 Young produced O O O 0 0 O 6 0 O 0 Adult mortality v_ \_ V_ L. i_ l_ v-~ v*_ V, t_ A a 0 0 0 0 0 0 Adult mortality u u. l_ v_ u v__ \ l-_ i_ l-Young produced o 0 O 3 0 0 0 Q Adult mortality v v_ \ L_ v__ I ^ u s 4 i<< s s s U s "A Adult mortality V. \^_ v_ I < v v_ <w 5 Young produced <<vi ^ K >>4 vS IT_ i4 VT_ \1_ >>T_ V- u v_ v_ L_ i_ v_ v_ i^_ w_ 6 o Ci b O 0 0 r> 0 o Adult mortality V_ u_ L_ l_ L_ u v^. \_ L_ ^ i<\ i<\ \\ T-to Tl M l-l TO T.T- VS Total young produced M VI <-U TA 4\ n Ht> an <<i<V v\ V V. \_ v- 1v_ V- v_ v_ \^ V-A'o/<f.- Adult mortality (L <<live. D = dead). S B = split brood (sinsle brccd spUt betweenlwt> da;*). CO*3 carry©vcr (offspring carriedover with adult during transfer). 0?. 2&.fa t -2101. ATI I - Exhibit ATI 1.2, revision 11-01-14 Page 41 of 91 Ceriodaphma dubia Client: TVA /Sequoyah Nuclear Plant Outfall 101 Date: 10-09-18 CONTROL-2 Survivaland Reproduction Data Replicate number Day 10 Young produced SL JQ_ O o SL D jCl Adult mortality v_ v_ Vv. ^SL SL n. o _Q_ u~ \^_ ^ o O SL £LQ_ _D_ .0_ ^_ 4 Adult mortality Young produced ID *o to IS, 11 >>o IT lO \\ V- v_ D Adult mortality v_- \^ .4 i+/- lid l\ is v* Tn "bT, 30 T* U\ n IfK Final Adult Mortality TT X for 3rd Broods 5£ ^ T v-^ y- I >- I 5gr JX^. (L=live. D* dead). SB* split brood (single brood split bctivccn Kmdays), CO

~~cany over (offspring carried over >vith adult during transfer).~~

Concentration: % Mortality: 07. Mean Offspring/Female: T^.T CONC: 100% Intake Survival and Reproduction Data Replicatenumber l Day 1 2 3 4 s c 7 8 9 10 1 Young produced n D o 0 1 0 o 0 O O rt Adult mortality \_ v_ v_ c_ u. L_ \- V_ L_ 2 Young produced 0 o O ft ft 0 ft 0 0 D Adult mortality L_ \_ V-. \_ v_

~~L_ v_ l_~~

Young produced 6 3 O o X) O 0 O o o o Adult mortality ^_ L_ v L_ v_

~~L_ v^ ^ v>_~~
~~j Young produced 4~~

^ u u S M lo n H ^ S Adult mortality \~ v_ \^_ V_ L_ \ u_ v_ v_ v_ 5 Young produced i^b IT. VT. IV IT l-b IC. >>3> IT. >>^> Adult mortality \_ \ \_ V_ t v^_ v_ v_ <^_ 6 Young produced O o o O O 6 O o 0 0 Adult mortality v_ L- \_ l^ L_ L_ ^. L_ i_ l~ 7 Young produced l6* T.O IS Tl Tl ii >>f H n n Total young produced si M 3t art sn 3& 3& a4 3S 36 Final Adult Mortality i_ V_ i_ l. i_ \- i_ <<_ >~ >>- Ateftv Adult inortality 0- ~ five;0

~~dead), SB = spfitbrocwJ (single broodspfitbetween two days). CO^carrvo ver (offspringcarriedover with adult duringtransfer).~~

Concentration: ( %Mortality: 07. 1Wean Offspring/Female: Aia' L-( Va Reduction From Control-2: "2M.0?. SOP ATI l - Exhibit ATI 1.2, revision 11-01-14 Page 42 of 91 TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16, 2018 ~j f 5 Verification of Ceriodaphnia Reproduction Totals EnWionroefltaiTeitlny Solution), lite Control- I 85.6% Replicate number Replicate rummer Day Totnl t>ay Total 1 2 3 4 S 6 7 8 9 10 I 2 3 4 5 6 7 8 9 10 <o 1 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 () 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 5 5 5 3 4 5 4 43 4 5 5 6 5 7 4 4 5 5 5 51 5 II 13 13 10 10 12 10 12 10 13 IN 5 12 14 13 14 12 13 13 14 12 14 131 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 14 14 17 13 12 15 14 15 IS 15 147 7 IS 17 22 IS 21 17 20 22 20 19 !94 Total 29 31 34 28 27 32 27 31 33 32 304 Iota! 35 36 41 37 40 34 37 41 37 38 376 10.7% 100% Replicate number Replicate number Day Totnl Day Total 1 2 3 4 5 6 7 8 9 10 I 2 3 4 § 6 7 8 9 10 1 0 0 0 0 0 0 fl 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 i 0 0 0 0 0 0 () 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 4 5 4 6 s 5 s 47 4 5 5 A 6 5 4 6 5 5 7 54 S 12 13 13 11 10 13 13 10 12 12 119 5 14 13 14 14 15 12 14 12 12 12 132 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 15 IS IS 15 20 17 15 20 21 175 7 19 19 21 19 21 21 20 20 22 (8 200 Total 32 33 35 33 30 37 36 30 37 38 341 Total 38 37 41 39 41 37 40 37 39 37 386 21.4% Control-2 Replicate number Replicate number Day > Total Day Total 1 3 4 5 6 7 8 9 ill 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 > 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 6 5 5 4 6 4 5 4 47 4 4 3 5 s 5 3 3 6 4 4 12 5 12 12 13 10 12 11 12 13 13 10 118 5 10 10 12 10 13 II 10 12 10 11 109 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 18 20 20 18 19 18 IS 21 19 188 7 16 It 14 12 14 16 15 II 15 14 141 Total 33 34 39 35 35 34 36 35 39 33 353 Total 30 27 31 27 32 30 28 29 29 29 292 42.8% 100% Intake Replicate number Replicate number Day Total Day Total 1 2 3 4 5 6 7 8 9 10 i 2 3 4 5 6 7 8 9 to I 0 0 0 0 0 0 0 0 0 0 0 J 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 6 6 4 4 5 5 6 5 49 4 5 6 6 5 4 6 7 4 4 5 52 5 14 12 13 13 13 II 13 14 13 11 127 5 13 12 12 11 12 13 10 13 12 13 121 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 i) 0 t) 0 (1 0 0 7 17 17 21 20 17 16 21 19 21 20 189 7 19 20 18 18 21 19 21 17 19 17 t89 Total 35 33 40 39 34 31 39 38 40 36 365 Total 37 38 36 34 37 38 38 34 35 35 362 TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16, 2018 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Environmental Testing Solutions, Inc. Verification of Data Entry, Calculations, and Statistical Analyses Project1 number: 13633 Concentration Replicate number Survival Average reproduction Coefficient of Percent rcitiictton from (%) 1 (%) (offspring/fcrmilc) variation {%) pooled controls (%) 2 3 4 5 6 7 8 9 10 Control - 1 29 31 34 28 27 32 27 31 33 32 100 30.4 8.2 No! applicable 10.7% 32 33 35 33 30 37 36 30 37 38 100 34.1 8.6 -12.2 21.4% 33 34 39 35 35 34 36 35 39 33 100 35.3 6.) -16.1 42.8% 35 33 40 39 34 31 39 38 40 36 100 36.5 8.7 -20.1 85.6% 35 36 41 37 40 34 37 41 37 38 100 37.6 M -23.7 100% 38 37 41 39 41 37 40 37 39 37 100 38.6 4.3 -27.0 Control -2 30 27 31 27 32 30 28 29 29 29 100 29.2 5.5 Not applicable 100% Intake 37 38 36 34 37 38 38 34 35 35 100 36.2 4.5 -24.0 Outfall 101: MSD = Minimum Significant Difference Dun iicit's MSD vjtluc: 2.576 PMSD = PercentMinimum Significant Difference PMSD: 8.5 PMSD is ameasure oftest precision The PMSD is the minimum percent difference between the control and treatment Unit can be declared statistically significant in n whole effluenttoxicity test. Intake: Dunnett's MSD value: 1.256 Lower PMSD bound determined byUSEPA (10* percentile) <<* 13%. PiMSD: 4J Upper PMSD bound determined byUSKPA (90* percentile) =47% Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from KPA's Win Intcrlqboratprv Variability Study (USEPA, 200liv. USEPA,2001b). USIiPA 2001a, 2U0IK Final Report. Inlcrlnbormory Variability Study ofl-PA Short-term Chronic mid Acute Whole Hffluent Toxicity Test Methods. Volume* Iand 2-AppendiN l-PA-821-13*01-004 and FPA-8">l-lM)M)OS US linvironmcnial Protection Agency, Cincinnati,011 TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16, 2018 Statistical Analyses Envtienmtntat 1<MUi*9 Sctattwtv tnc. Ceriodaphnia Survival and Reproduction Test-Reproduction Start Dale. 10/9/2018 Test ID: CdFRCR Sample ID: TVA / SQN 101 End Dale: 10/16/2018 Lab ID ETS-Envif. Testing Sol. Sample Type DMR-Discharge Monitoring Report Sample Date: October 2018 Protocol: FWCHR-EPA-821-R-02-G13 Test Species: CD-Geftodaphnia dubia Comments. Non-treated Conc-% 6 8 10 Control-1 29.000 31.000 34.000 28.000 27.000 32.000 27,000 31.000 33.000 32.000 Control-2 30.000 27 000 31.000 27.000 32.000 30 000 28.000 29.000 29.000 29.000 10.7 32.000 33.000 35.000 33.000 30.000 37 000 36 000 30.000 37.000 38.000 21.4 33.000 34 000 39.000 35.000 35.000 34.000 36.000 35.000 39.000 33.000 42.8 35.000 33.000 40.000 39.000 34.000 31.000 39.000 38.000 40.000 36.000 85.6 35.000 36.000 41.000 37.000 40.000 34.000 37.000 41.000 37.000 38.000 100 38.000 37 000 41.000 39.000 41.000 37.000 40.000 37.000 39,000 37.000 intake 37.000 38.000 36.000 34.000 37.000 38.000 38.000 34.000 35.000 35.000 Transform: Untransformod 1-Tailsd Isotonic Conc-% Mean N>>Mean Moan Min Max CV% t-Stat Critical MSD Moan N-Moan Control-1 30.400 1.0411 30.400 27.000 34-000 8.235 10 35.417 1.0000 Control-2 29.200 1,0000 29 200 27.000 32.000 5.546 10 10.7 34.100 1.1676 34100 30.000 38.000 8.572 10 -3.284 2.287 2.576 35.417 1.0000 21.4 35.300 1 2089 35.300 33.000 39000 6 127 10 -4.349 2.287 2.576 35.417 1.0000 42.8 36.500 1 2500 36.500 31.000 40.000 8688 10 -5.414 2.287 2 576 35.417 1.0000 85 6 37.600 12877 37.600 34.000 41000 6.417 10 -6.391 2.287 2576 35417 1.0000 100 38.600 1.3219 38.600 37.000 41000 4.266 10 -7.278 2.287 2.576 35 417 1.0000 Intake 36.200 1.2397 36.200 34.000 38.000 4,473 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0,83947 1.035 -0.0678 -0.905 Bartlett's Test indicates equal vanances

100 1 2.57619 0.08474 85.9767 6.3463 1.4E-08 5.54 Treatments vs Control-1 Linear Interpolation (200 Rosamplos) Point % SD 96% CL Skew 1C05 >100 IC10 MOO IC15 >100 IC20 MOO IC25 MOO IC40 >10Q IC50 MOO 50 100 Dose % Dose-Response Plot 1-taii 0 05 level of significance Page 45 of 91 TVA / Sequoyah Nuclear Plant, Outfall 101 Intake October 09-16,2018 Statistical Analyses Environmental Testing Solution*, Inc. Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 10/9/2018 Test ID: CdFRCR Sample ID: TVA/SQN 101 - Intake End Date: 10/16/2018 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: October 2018 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Certodaphnia dubia Comments. Non-treated Conc-% 6 8 10 Control-1 29.000 31.000 34.000 28.000 27.000 32.000 27.000 31.000 33.000 32.000 Control-2 30.000 27.000 31.000 27.000 32.000 30.000 28.000 29.000 29,000 29.000 10.7 32.000 33.000 35.000 33.000 30.000 37.000 36.000 30.000 37.000 38.000 21.4 33.000 34.000 39.000 35.000 35.000 34.000 36.000 35.000 39.000 33.000 42.8 35.000 33.000 40.000 39.000 34.000 31.000 39.000 38.000 40.000 35.000 85.6 35.000 36.000 41.000 37.000 40.000 34.000 37.000 41,000 37.000 38.000 100 38.000 37.000 41.000 39.000 41.000 37.000 40.000 37.000 39.000 37.000 Intake 37.000 38.000 36.000 34.000 37.000 38.000 38.000 34.000 35000 35.000 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% t-Stat Critical MSD Control-1 30.400 1.0411 30.400 27.000 34.000 8.235 10 Control-2 29.200 1.0000 29.200 27.000 32.000 5.546 10 10.7 34.100 1.1678 34.100 30.000 38.000 8.572 10 21.4 35300 1.2089 35.300 33.000 39.000 6.127 10 42.8 36.500 1.2500 36.500 31.000 40.000 8.688 10 85.6 37.600 1.2877 37.600 34.000 41.000 6.417 10 100 36.600 1.3219 38.600 37.000 41.000 4.266 10 Intake 36.200 1.2397 36.200 34.000 38.000 4.473 10 -9.666 1.734 1.256 Auxiliary Tests Statistic Critical Skew Kurt Shaptro-Wilk's Test indicates normal distribution (p > 0.01) 0.92285 0.868 -0.0072 -1.1393 F-Test indicates equal variances (p = 1.00) 1 6.54109 The control means are not significantly different (p = 0.22) 1.27279 2.10092 Hypothesis Test (1*tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.25578 0.04301 245 2.62222 1.5E-08 1, 18 Treatments vs Control-2 Dose-Response Plot 1-tail. 0.05 level of significance Page 46 of 91 ?!'-; I 3 trtwrawvnul UtangietaOatA. Inc. Page 6 of7 Species: Ceriodaphnia dubia Date: 10-09-18 Client: TVA / Sequoyah Nuclear Plant OutfaU 101 Day (Analyst identified for each day* performed pH, D.O. and conductivity measurements only.) 0 1 2 Analyst fQ MS. HS T3 TS r° Concentration Parameter 'tell' ~rx~X~£'~ i" ?* ?"<; j". "* -. S3 £7&',i.-:2 pH (S.U.) ~7.&<? ^.ez 7.:fcr 7. gO 7.5? ^.S0 DO (mg/L) 7-7 7.9 7.S 7.9 "7.^ Ofl Conductivity (jimhos/cm) 3/<? 3)0> 305 CONTROL *Alkalinity (mgCaCOa/L)
Hardncss UO

£<<A V>?\ ':>//f4ji':/yl" ^<<t 80 (mgCaCOs/L) ^Temperature (°C) tH-t -^.v "bV* T.S.X Ti^.l -U4.-8 pH(S.U) *7.qt, 7-#i 7-ff/i, -7.20 7.7? a,<<s6 DO (mg/L) 7.1 &o 7-er 7.9 T:? a^> ^if-'^ir.'^ 10.7% Conductivity **.'.-""F'.wv"".- (umhos/cm) Z8o 30S9 ^79

Temperature (°C) *vl-S ( "LS.\ ->>M *\£/D vi.-\ *v\.f pH (S.U.) 7*3 TJgto 7.(33 T.79 7.K '*^n-DO (mg/L) l?-*? #. 7.9 7.<<>> T:^ 'w 21.4% Conductivity .* *^"ft"^'<<*<^i*: .* w'~

(umhos/cm) Z&H 5 :.ji'~:C~'.'& -ZQs- ;'" "**'.' "-'-1'- j ^ 3 ^Temperature (°C) *MK-& *^AA *vM -vH-i TA-T TA-S pH (S.U.) ^.sn 7.79 7.S">> 77f -Z6? ^-^ DO (mg/L) ^9 £>> 7.*? &o ~7.<r ao 42.8% Conductivity w .r-it^riv V>>;'t. 24*? %>Z (^mhos/era) >4ilS^'>fel,.*.' ^Temperature (°C) TAA "IS-\ T&.0 -uK-4 ^*n TM.-S pH(S.U.) "f-C>>o 7.^ 7.7*r 7.75 -Z57 a<<sh DO (mg/L) &o #t £* £i 7^5 e.i 85.6% Conductivity (urahos/cm) /65~ i^;?:^:i W3 /<J0 ^Temperature (°C) TH-T 1S--L IS-D *y&.t- -L4.i "IS-O pH (S.U.) ^SS" 7-^3 7-73 7.7M 7.5-/ a.uft DO (mg/L) 8.Z Si £l i?H 7-i? ©.1 Conductivity (Mnihos/cm) ts? l;:f.:.-vS?:r;v^r /V? m m^ Alkalinity 100% (mgCaCOs/L) S<\

~~Hardncss (mgCaCOj/L) sA sg~~
~~TR chlorine (mg/L) *o.io ?* \ A2 '*'^j.'^'^i'V *Ti ^ojo 5^FJ-i?^; ?*'~~
Temperature (°C) T£-D 14-<f 1S.D TH-/ "W.^ *fl.*

pH (S.U.) ^*57/ 7.7H "7-^6 *7.70 7HH au^ DO (mg/L) S* &3 fi.'S 8.Z 7-tf 0,0 Conductivity tf? /^ ';**_* .^fe^-j /3^f (umhos/cm) d^lSyj-'C^i: 100% ^Alkalinity Sto -.'.....*-VlA^i-. -SL. Intake (mgCaC03/L)

~~Hartlncss (mgCaCOVL) s* Sl-~~
~~TR chlorine (mg/L) ^O.lQ ^taLtofi sssmm ^O.'O ^^i^^~~

^Temperature (°C) 1 TS.O TS.O TS-0 is.o 1+1 ^.H J Initial Final Initial Final Initial Final Temperatures performedat the time of test initiation,renewal or temiination by the analyst identified in the Daily Renewal Information table located on Page I. Alkalinity, hardnessand total residual chlorineperformed by die analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: \A SOP ATI 1 - Exhibit ATI 1.2, revision 11-01-14 Page 47 of 91 n=3c?pi s . " J (mfaBfteMMJtTtitiftgScfcixtenLbe. Pa°e 7 of7 Species: Ceriodaphnia dubia Client: TVA / Seauovah Nuclear Plant. Outfall 101 Date: 10-09-18 Day (Analyst identified for each day, performed pH, D.O. andconductivity measurements only.) 3 4 5 6 Analyst YU esu TS TS MS A*F Concen tration Parameter " v#C ,^ -,'v .! -1 .*" ~ . "... >r ' pH (S.U.) ^i T-.uo >.6o 7.6? 7.70 7-^^ "7.V9 7.6/ DO (mg/L) \% *2,o >X ?.o 7.7 ^B 7.7 ¥.? Conductivity ,"; *C- '.." -'**' (umhos/cm) 3oo l>'^*"* ^h?^^ 5/3 313 CONTROL *Alknlinity *.^p-.'^f^.T "*£A7, **;-. .-* (mgCaCOj/L) ^e A\ 58 .*--.*..->-7:>>r.^ X.* /JJS-^S?^ VJSi^ ^£&] '^x

~~Hardness (mgCaCCb/L)~~

&o ^Temperature (eC) -t*\..-\ lAA "v<<\A -i&.\ "^4 -l&JSLm. *H.t I.S.T-pH(S.U.) 1^4 T-.S8 ~%.\jifi 7.69 7.^0 7.V/ 7-so 7.51 DO (mg/L) 8.o 8./ ?.l 7.9 fco ?.\ 1.9 flo 10.7% Conductivity (umhos/cm) a<^ Q1A *' '*--!-, 4 JL93 -**-r'^i.-Vv.*^ 2f& ^Temperature (°C) -e\?<< W.fe -^*^ ~iM.f \<<\.<< 1H.<i "LH-i *v\.t pH(S.U.) \-*5 "*,'5B) uz 7.68 7.77 l.HZ 7.V5 7.H8 DO (mg/L) 0.Q S-1 S.j <<-l ^?.l fto 79 S.\ Conductivity ^^i^i?! 21.4% (umhos/cm) frTi .':* ;5,. 1-V <3u5 <?7? 2?7 ^Temperature (°C) -^rA -\A.k *u.^ TS.V -vl. t -v&.\ -li\.£ xH-e pH(S.U.) aiA T-.s-?- T-.Sl

7. $7 7.73 ^l 7.^5" 7MH DO (mg/L) 0.1 <&.o <&-t ^.l 9. a (^O 7.^ *\

42.8% Conductivity (umhos/cm) olHO ;*:', j'.^-;.,"^! 23* ^/5 ZHZ

Tcmperature (°C) -\A.$ -\S.O ^."l Tj\A *vA-8 -tH.f SiK't -iM-1 pH(S.U.) O.t/>>o T-.S5 *^-.Sll. 7.64 766 7.Yo 7.29 7MO DO(rag/L) &.I ^ <&.l ?.l 5?p <<?l <<i7 &\

85.6% Conductivity r--v *" vi: (umhos/cm) rr-b M > 4..S. >>jr*^>> >> 111 ".- ".',^.>?- - sv /?/ Temperature (°C) -^.f- -iA.fr \<<\A "Vj4.^ lA-f 1S.0 "U\-S -is.l pH(S.U.) ^.*51^ -i.fia >.4<* 7.Co£l -7.te?> 7.35 7-36 7-35 DO (mg/L) 6.2 ?,$ <ai ^-a ^.3 #.i #A 8.-L Conductivity s \ '*?;" * \ . \* (umhos/cm) 1*45 IM m ^v^iV<<^J-Tr. &H ^Alkalinity 100% (mgCaCOa/L) >: ' ,/'*>*"* '" *,'";'*" *,>> '* st~ i*v ":'X*: *I

~~iJ "*- -' /.vj."'^ ^j>>: ,"l" ** . * ^.j~~

~~%^-*' ' 'p.'.-.- ;*- - " j " ^~~

~~Hardness (mgCaCO-j/L) 14to'<< _/ 4*. ,/f^>>§. ?.??( .';'\'F ' *_,'.>. J t^*ivifj.r~~
~~TR chlorine (mg/L) 'v .* "'w* >>* ****. fir-r Y^'/JS <0.l0 * .'^"'V^vV V * .'J.Sl" " i .!! S!'JSS£l: L^3r27'5*Vi~~
Temperature (°C) ~\s>>.o M-S TS.O *bl^ -lAA "IS.D TH-n XH.1 pH(S.U.) %<5V "kS/ 7/+2 -7.&-? 7.&A ^.39 7^?& 7M DO (mg/L) d/ >& 9.>> Tjr.^ <<.a <<r ff.i &Z.

Conductivity (umhos/cm) m^ \3\* "_t("^vr^:; .j /^7 /*// V^v} _.**'; 100% *AlknIinity B7'.,.'.7.., Intake (mgCaCOs/L) l.r^M;4 t wisJuS-*<<\ - 'i*J>>i St- 6}"~. ^x l^fi"^^ -~-^-.~-^ ~< ^Hardness (mgCaCOj/L) *\ *7 t\~~ "~ tV( *r

~~TR chlorine (mg/L) rJV - -; ^T^-V -It ! <0*l0~~

- ~:'".J'r~5M'"AS5 -J*^^^*'- -i- e "*^f* ^ ~*) "-.?_ '.**--C^ -^ i." *,c ^Temperature (°C) T4.<\ VM i>\.e 14A m..n iH.ff Vl.H TS\ Initial Final Initial Final Initial Final Initial Final

Temperatures performed at die lime oftest initiation, renewal or tennination by the analystidentified in the Daily Renewal Infonnationtable located on Page L Alkalinity, hardnessand total residual chlorine perfonned by the analyst identified on the bench sheet specific foreach analysis and transcribedto this bench sheet by:

Page 48 of 91 SOP ATI l -Exhibit ATI 1.2, revision II-0M4 TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated October 09-16,2018 s <Q CD Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-0I3, Method 1002.0 O Daily Chemical Analyses \ : Project number: 13633 Environmental Testing SoluUons, Inc. Concentration Parameter Day 0 Dav 1 Dav 2 Day 3 Day 4 Day5 Day 6 initial Final Initial Pruul Initial Final Initial Final Initial Final Initial Final Initial Final pll(SU) 7.09 7.82 7.75 7.80 7,59 7.58 761 7.60 7,50 7.69 7.70 7.40 7.49 7.61 OO (niR/L) 77 7.9 7.8 7.9 7.9 7.9 7.7 8.0 7.8 8.0 7,7 7,8 7.7 7.9 Conductivity (umhos/cm) 319 316 302 300 297 313 313 Control Alkalinity (mjj/L CaCO^) 60 59 58 Hardness (nig/L CaCOj) 84 80 8(1 Temperature fQ 24.7 25.1 24.8 25.1 24.7 24.8 24.7 25.1 24.8 25.0 24.8 24.9 24.7 25,2 pH (SU) 7.76 7.81 7.S6 7.80 7.78 7.58 7.74 7.5S 7.66 7.69 7.80 741 7.50 7.51 DO (me/L) 7.9 8.0 7.8 7.9 7.7 8,0 8.0 8.0 8.1 S.l 8.1 7.9 7.9 SO 10.7% Conductivity (umhos/cm) 280 308 279 296 282 293 296 Temperature ("C) 24,8 25 1 24.9 25.0 24.7 24 s8 24.8 24.8 24.9 24.8 24.8 24 9 24.8 24.8 pM (SU) 7.73 7.80 7.83 7,79 7.75 7.57 7.75 7,59 7,62 7.68 7.77 7.42 7.48 7,48 DO (me/D 7.9 8,1 7.9 7.9 7.7 7.9 8,0 SI 8.1 8.1 8.1 8.0 7.9 8.1 21.4% Conductivilv (jimhos/cm) 264 285 262 272 265 279 277 Temperature (°C) 24.S 24 9 24.9 24.8 24.7 24.8 24.8 24.8 24.9 25.1 24.8 25.1 24,8 24.8 1)11 (SU) 7.69 779 7.81 7.79 7.69 7.56 7.67 7.57 7.59 7.67 7.73 7.41 7.45 7.44 DO (me/L) 7.9 8.1 7.9 8.0 77 8.0 8.1 8.0 8.1 8.1 8.2 8.0 7.9 8.1 42.8% Conductivity (umhos/cm) 234 249 232 240 232 245 242 Temperature ("C) 24.9 25.1 25.0 24.8 24.7 24.8 24.8 25.0 24.9 24 9 24.8 24.S 24.8 24.7 pll (St l) 7.60 7.74 7.75 7 75 7.57 7,50 7.60 755 752 7.64 7.66 7.40 7.39 7.40 DO (mg/L) 8.0 S.l 8.0 8.1 7.8 S.l 8.1 79 SJ 8.1 8.2 8.1 8.0 8.1 85.6% Conductivity (pmhos/cin) 165 173 160 173 169 177 171 Temperature CO 24.9 25.2 25.0 25.2 24,8 25.0 24,8 24.8 24.9 24.9 24.8 25.0 24.8 25.1 pll (SU) 7,55 7.73 7.73 7.74 7.51 7.48 7 56 7.52 7.46 7.62 7.63 7.38 7.36 7.35. DO(ntR/L) 82 8 1 8.1 8, J 7.8 8.1 8.2 7.8 8.1 8,2 8,2 8.1 8.1 8.2 Conductivity (umhos/cm) 139 147 134 145 139 148 144 100% Alkalinity (mg/L CnCOj) 54 50 52 Hardness (mg/L CaCOj) 54 50 54 Total Residual Chlorine (mg/L) <0I0 <0.I0 <0 10 Temperature ("C") 25.0 24.8 25.0 24.8 24,8 24.8 25.0 24.8 25.0 24.9 24.9 25.0 24.7 24.9 nH(SU) 7.51 7.74 7 76 7.70 7.44 7.42 756 7.51 7.48 7.62 7.62 7.39 7.36 7.32 DO(mR/L) 8.2 8.3 8,3 8.2 7.8 8.0 8 1 7.8 8.1 8.2 8.2 8.1 8.1 8.2 Conductivity (umhos/cm) 137 148 134 142 136 147 141 100% Intake Alkftiinllytiufi/LCaCOj) 56 52 52 Hardness (mg/L CaCOj) 58 52 54 Total Residual Chlorine (me/L) *'O.m <().I0 <0.10 Temperature (*Q 250 25,0 25,0 25.0 24,9 24.9 24.9 24.9 24.8 24.9 24,7 24.8 249 25 1 File sqnl01 _ 100918chem Entered by T Sleeper Reviewed by

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Page //? I?>>v>>roft<<<<f>>firit<<ttrtg Solu^onk tne. Page_/ of l_ Total Residual Chlorine (4500-C! 6-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL =0.10 mg/L Analyst CHM IQ\~l J&. DPD: Date analyzed l(\>(ft>\% Positive and Negative Control: Control Type Sample ID Result K) Positive Negative Negative control Deionized water ^ Positive control Tap water V Sampie screening: Sample Sample ID Sample characteristics Result (V) number Positive l Negative f/QM" ytlloiAtckur l&lOOl.CTL- T\/PA<^3 lO\ v/ \&ioo4.Cb 4/ i*Wfct£ //<jh+ yclloio.c/earj paHjeles \S i£>>oo£.0 \ B^\tf2e%oS> //gM +00, ckar, {taftlctcS f no cofar, clear ifiDCW.Q^A "fy^U u>njT* (* V l^/DO^-tS no ctbf, clear *" R\e>^\uX iS/DO^-ft^ Msk\JLX //5M -ten. clear w" isidcA-gr- Cft<KFrf^O0#\ ye//ou>, e'eft/-, particles \S ItiOO^O^ Li\HfUfrtVpe^- //gh+ +Qf\ clear V* 1*100*. oV p<<VWAl^ ^ght -rao, c/car;parhdcS \s* ^ ^^^ ^^ ^^ < tio-*> ^ ^^" ^^ ^1 . 1 Note: All samples were analyzedin excessof EPA recommended holding time (15 minutes)unlessotherwise noted. A positiveresult Indicatesthe presenceof total residual chlorine above detection{> 0.10 mg/L), which resultsina pink to red colorchangewith the addition of the DPD indicator. A negative resultindicates the absenceof total residual chlorine, which results in no colorchange withthe addition of the DPD indicator. Reviewed by n Date reviewed JQ-64-if Page 50 of 91 SOP C8-Revision 4- Exhibit C8.1 Page 5H Page J of l_ Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L, Meter: Accumet Model AB250 pH/mV/lon Meter Analyst T5 Iodide reagent: 'MR %Q Date analyzed /O'09-l% Acid reagent: 'NR %2>5 Caiibration: Slope (mV) 0.10 mg/L 1.00 mg/L (suggested range =26 to 30 mV) Reference standard # INSS /6/7 INSS /#/7 *? Note: For samples with a residual chlorine of >1.0 mg/L, the samples must be diluted to be within the calibration range. Laboratory controi sample: Reference standard True value (TV) Measured value (MV) %RS = MV/TVxl00 number (mg/L) (mg/t) (acceptable range =90 to 110%) INSS /(op 0.50 0.552 /66M% Duplicate sample precision: Sample number Sample ID Total residual chlorine %RPD = {(S - D) /[(S+D)/2]}x 100 (mg/L) (acceptable range = +/- 10%) \k Ibfcfi.Cft- WJsfttaifcY 5 6.00%

~~Duplicate -ts /a.o9./e_~~

I D O.0O& Sample measurements Sample Sample ID Total residual chlorine number (mg/L) TV < 0.05 mg/L Method Blank (MB) *0. 0/0 TV = 030 mg/L Method Detection Limit spike sample (MDU) O&Ll \*iO0<i. £h -TMK\<£}*i> 1^\1KV& 60.003 /^ / ^ ^ S" / ^-^ ^-" _ 1o ^"^ w* r0^ -f1^ ^^ ^^ <<^r . Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control sample: Reference standard True value (TV) Measured value (MV) %RS = MV/TVX100 number (mg/L) (mg/L) (acceptable range =90 to 110%) INss /&j7 0.50 C.57 7 /03.H% Reviewed by Datereviewed l /fl-O^-if Page 51 of 91 SOP C8-Revision 4-Exhibit C3.2 Page_£M_ EftvifBfua*aufTejtt<<9Sdutbott, tec Page V of I Total Residual Chlorine (4500-CI G-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L Analyst +/- CHM DPD: ion Date analyzed JQ-HM& Positive and Negative Control: Control Type Sample ID Result K) Positive Negative Negative control Deionized water */ Positive control Tap water w" Sample screening: Sample Sample ID Sample characteristics Result K) number Positive Negative \<£>>o!D-^>\ ^/\\<>0^\M PfrLfi. Tf\v\* Cv&Ne.. -**- \ fottvCAjeS <*/ tfrlOiO.YL-* \j i^TftvCe^ \S A / / / / / / / / / / / / / y C- Yt tl Note: All samples were analyzed in excess of EPA recommended holdingtime (15 minutes) unless otherwise noted. Apositive result indicates the presence of total residual chlorine above detection (> 0.10 mg/L), which results in a pink to red color change with the addition of the DPD indicator. A negative result indicates the absence of total residual chlorine, which results in no color change with the addition of the DPD indicator. Reviewed by TL Date reviewed lo-io-i8 Page 52 of 91 SOP C8-Revision 4- Exhibit C8.1

/*-*- - *.. I , *
~~J Page. SB Page / of f_~~

Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L, Meter: Accumet Model AB250 pH/mV/lon Meter Analyst Iodide reagent: tmjfe *:* Date analyzed Acid reagent INR m Calibration: Slope (mV) 0.10 mg/L 1.00 mg/L (suggested r.inge - 26 to 30 mV) Reference standard t) INSS /£>/? INSS It, '3 *?& Note: for samples *iM\a residual chlorine of > 1.0 mg/L, the sample?: most be diluted to be within the calibration rang??. Laboratory control sample: Reference standard True value (TV) Measured value (MV) %RS = MV/TVxlOO number (mS/L) [mg/L] l;n-7eptarjie range = 90 to 510%) INSS /(,/*£ 0.7.0 0.5"3C/ Afl£. tf% Duplicate sample precision: Sample number Sample 10 Total residua! chlorine V'.'KPO = {(S - D) /[(S+DJ/2J] x 100 (mg/L) (acceptable range = i 1074) itlOIO. 1 t *TM*\lfC ftftl S lu.°ZJ /-</' & Duplicate D £&.o\6 /pyJ/ £3 Sample measurements Sample Sample ID Total residual chlorine number (mg/L) TV < 0.05 mg/L Method Blank (MB) ZjO-o&? TV = 0.30 mg/L Method Detection Limit spike sample (MDLs) 0.3Z2 ) i IDlO.7.4 T\l

~~ucjt u^e.L t £ £->. £"-^/~~

l£ibl&.%\ TsJ/\\ Saw mv Z<0-<<>>7 liioiO.SX- 2L lUrwtt lO.O£>3 Y / / __ _ ... / 1 / ' r / f\ An / r? ~~/ / / A I/O/J? / ° / <<" >'CJ / / ~y~ j£*__ . Note.- Allsamples were analyzed it? excess of FPA recommended holding time (15 minutes) unless otherwise note Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS - MV / TV X100 number (mg/L) tmgAJ (acceptable range = 90 to 110%) INSS )ty-$ 0.50 O. <<>2 Joo-^^h Reviewed by Date reviewed /0-10-lf

8*

Page 53 of 91 SOP CS-Revision 4Exhibit CS.2 Page 11*5 fovfeenmsstit VotingSetntfitafc'"'* Page \ of_J, Total Residual Chlorine (450Q-CI G-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L Analyst ZS_ DPD: CUM i0v^ Date analyzed io-yb->fc J Positive and Negative Control: Control Type Sample ID Result K) Positive Negative Negative control Deionized water sT Positive control Tap water ** Sample screening: Sample Sample ID Sample characteristics Result (V) number Positive Negative //<jM yellow, c\<uxr) Y^hrJCTi l/ \<£icm..ib TVKv^aw \o\ //ah+ yc//c^\ ctor\ car-ficlcS Uit>\-i-x4 *I lt*\7\K-cL tX* /r^h-r- yt.tt<ju>, clear, r^ar-bclcs ^ MwaWllL- AUbAe*a<> /ffjhV- tan, clear"

~~UtttS . csh> "bONN> docOtP 00 Co/or, clear IiiO^.0-\ &*&tviiuLcL~~
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//ghr vc/,W>, c/ei..' S i<iiDv^>.cru CAPST"P*i OO&A \ld/ot>>,^/'5h^ c/cyci./( ()arHdcs V jAH p it r-UTfetCa =i /*ghV yci/Ok> t cVcar s /£ i Oi i>.D\ B^THPfci ^J.**"- ^^ ^__^^-^

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\ ° ^ -> // y ^y^ Wote: Allsamples were analysed in excess of EPA recommended holding time (15 minutes! unless otherwise noted. A positive result indicates the presence of total residual chlorine above detection (> 0.10 mg/L),which results in a pink to red color change with the addition of the DPD indicator, a negative result indicates the absence of total residual chlorine, which results in no color change with the addition of the DPD indicator. Reviewed by Date reviewed ¥ iSkJJzliL Page54of91 SOP C8-Revision 4- Exhibit C8.1 Page S U Page l_of !L Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L, Meter: Accumet Model AB250 pH/mV/lon Meter Analyst TS Iodide reagent: INR %0 Date analyzed 10 */&*/? Acid reagent: INR $35 Calibration: Slope (mV) 0.10 mg/L 1.00 mg/L (suggested range = 26 to 30 mV) Reference standard # *nss /6 / 7 INSS /#/7 <<?? Note: For samples with a residual chlorine of > 1.0 mg/L, the samples must be diluted to be within the calibration range. Laboratory control sample: Reference standard True value (TV) Measured value (MV) %RS = MV/TVxl00 number (mg/L) (mg/L) (acceptable range =90 to 110%) INSS /(fill 0.50 0557 /Q*1*A Duplicate sample precision: Sample number Sample ID Total residual chlorine %RPD= {(S - D) /[(S+DJ/2]} x 100 (mg/L) (acceptable range =+/- 10%) iitnllTl^> "TvIAvUg^ lOV. s 6.00% X 1Duplicate o 0.O07 im> JO'l&.HBr Sample measurements: Sample Sample ID Total residual chiorfne number (mg/L) TV < 0.05 mg/L Method Blank (MB) Ln.ota TV = 030 mg/L Method Detection Limit spike sample (lyiDLs) 0.313L IfclOVT-.lA 'Xvlft^Gv^ tw>rMft- t-O.OOA - .....^^r ....l ^ ^ ^ ^^ ^y^ 10" ^ ,V^ \^>- 'OS ^ y ^^ Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control sample: Reference standard True value (TV) Measured value (MV) %RS = MV/TVxl00 number (mg/L) (mg/L) (acceptable range =90 to 110%) INSS /£/7 0.50 0.537 105A V* Reviewed by 4t Date reviewed l /0 ^H<f Page 55 of 91 SOP C8-Revision 4HExhibit C8.2 Page *<*$* Page, j of J_ ^ Analyst vO Alkalinity (SM 2320 B-2011) Date analyzed 1P.0*H<<6 Matrix: Water, RL =5.0 mg CaCOa/L, Samples are titrated to pH =4.5 S.U. Titrant normality and multiplier determination: Titrant reference Normality check Begin End Total Normality (N) of HzSOa pH Factor or Multiplier number standard number mL mL mL =(5mLNaaCO* x 0.05J/E =0.25/E =(rV x 50000)/100 mL sample (acceptablerangeg 0.0180- 0.0220) **Vx500 INR &£{0 'nss y${ 12.4 0,0202-0*t> £4 ia/ Laboratory control standard (LCS): Reference standard True value (TV) Sample Begin End Total Multiplier Alkalinity (MV) %RS=MV/TVxl00 number (mg CaC03/L) volume mL mL mL (mg CaCOa/L) (acceptable range =90 to 110%) (mL) INSS I to~bQ 104 <*** <**M 100 100 I2.H 7Z.Q °\^ Duplicate sample precision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOj/L) *{(S-D)/<<S+D)/2]}xlO0 number (ml) mL mt mL (acceptable range =+/- 10%) id.o&& Ssuu Joo &(<\ %s*a 3.0 tai s 3o \ Duplicate (D) J faO se\ 3,l 4 D 31 z.zt Sample measurements: Sample Begin End Total Multiplier Alkalinity Sample number Sample ID volume (mL) mL mL mL (mg CaC03/L) MB (TV<2.5 mg/L) Deionized water. dH = 4."*> s.u. 100 0.O O.O O.O I0.I MO I O. D>>. 10 66wJ / Se.t 3I.I 3.0 I $n \ D -02* +/-0 mitsuj Jl.S" 3>H s.^ vo O^atf.^fl- \ 3^.vi ^32. *o^ &\ 01-&IaJ©P>> 1 <B.£ <<4^Z {0.0 U/ UlooM' ol CjNtmh&dU

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Laboratory control standard (LCS): Reference standard True value (TV) Sample Begin End Total Multiplier Alkalinity (MV) %R5 =MV/TVxl00 number (mgCaCOs/L) volume mL mL mL (mgGaCOj/L) (acceptable range << 90 to 110%) (mL) l04 q^ asr.ov, INSS <<o3-6 100 100 <&*<0 3<aO <M Reviewed by: 5C Date reviewed: 1 /^yffl-i<fr Page 56 of 91 SOP C6-Revision 4-Exhiblt C6.1

~~- Page <*>>,~~

i Page 1 of _g^ Analyst 8Ss Alkalinity (SM 2320 B-2011) Date analyzed Matrix: Water, RL - 5.0 mg CaCCb/L, Samples are titrated to pH - 4.5 S.U. J'S^iriiLS_ Titrant normality and multiplier determination: inr3.ni reference Normality check Begfn End Total Normality (N) of H;SO.-. pH Factor or Multiplier number standard number mL ml ml * (5 mL NaiCOl x 0.05)/E

0.2.5/E * (Wx50000)/ too mL samph (E) (acceptable range << 0.0180 - 0.0220] sNjfSOfl INR %&, lNSS i^SA- C.t> I2.D V2..0 0.O2-C2 -o4 Laboratory control standard (LCS):

Reference standard True value (TV) Sample Begin End Total Multiplier Alkalinity (MV) K RS s MV / TV v 100 number (mg CnCOj/l) volume nit. mL mL (mg CnC03/L) (acceptable range - 90 to 1105-51 (mt) INSS ll/3<s 100 100 Ii.o <<?>>.H *.h io.*f s-s *J?.otoi>> Duplicate sample precision: Sample Alkalinity '.rpi > Sample Sample ID volume Befiin End Total Multiplier (mg CaCOi/JJ = {(S-Dl/((S<<-D)/2])xl00 number (mL) mL ml nl (acceptable range = i 10! io>ue>.ie& *-<</3H-*\j l©° &IA <<?f.3 &A tort 3o D I Duplicate- (D) J- **£_ J. &<^ lo.lj..? ?IA Z<\ So Sample measurements: SBfif K>.l9.t< lMS2£3-ld Ojnrv UluYlP t JiOq_ i^.H- hS.4 QjD 2L IE-ion. oS 1SA. iiJL o.T- JU>_ lfcj&a^&L ; 3 i4J ^*3 l-2_ 12. lfl<0 tow 8 iSi&a!uol_ rCm,rr\M, 111 ioo 2o.3> o-u.u tf-S 3w>\* S3- +/-r iLCULjH 3.U, t flo-*- tRfOI?>>.0' J 3>t?.i 5A.T- AS _3te_ -JiirD6^.0W- H^tmili- t _5b_ i3a>>3: SkJ 3* us *j

t g (Oil -Ok 3C-\ h\.* +/-b3Uia )DO 1&IDI^.C6 3 H4.o **s.% 1>*^ dg ^^iCL1 ei Laboratory control standard (LCSJ: H>>2. 101450 "f^kH-^mx^^tandard 1 Truevalue(TV) Sample Begin End Total Multiplier Alkalinity (MV) % RS << MV / TV X100 number P~TjnTr&>>{7<5;./U__ __volume mL mL mL (mg cacoj/L) (acceptable range - 90 to 1105i)

(ml) __ INSS E>3l tc.^.iS 100 100 1 Reviewed by: A, Date reviewed: l lQ.m.L£> Page 57 of 91 SOPC6-Revisior,4-txhibi! C6 I Page 4-T Page A of 5> Analyst Alkalinity (SM 2320 B-2011) J&* Date analyzed Matrix: Watet, RL = 5.0 mp, CaC03/L, Samples are titrated to pH -- 4.5 S.U. IQ-^.ig Titrant normality and multiplier determination: TitrSnt refr:rr<<ir! Uoaiia!iUi_check Uegin End Normality [rjj of rfeStfc pH Factor or Multiplier number standard number- <(5 mL Na?COj x 0.0S)/E = 0..75/E (W x SOO0O)/ 100 mL sample ~tatTOpteble-Kia6£-aiL018Q - 0.0220) -<<x 500 iN H INSS Q&i io-v^.q Laboratory control standard (LCS;- Reference standard True value (TV) Sample Blgjn End Total Multiplier Alknlinity (MV) % ItS a MV / TV x 100 number [mg CaCO>/L) volume mL mL 117L (mg CaCOj/L) (acceptable rang?.- = 30 <o 13f:."t) (mL) mss &V3S 100 100 D.O cj.4 <<w I0.4- <*8 %.0°fo Duplicate sample precision:

mple Alkalinity 55RPO Sample Sample to volume Begin End Total Multiplier [tng taCOs/L) =<<5-D)/((S+O)/2],xlO0 number (ml.) ml mL (acceptable range = +/- 105>>)

~~i£ioo°\.o\ &ftbbna'~\ I 26 <<*M a.M- Z.o (#v> 10.4 17<7~~

~~1. Duplicate (D) -i- QM- iS.<4 3.o t0 -i- D~~

&$/_ -jo.ia-l't Samole measuremantb Sample Begin End Total MuJUpHei Alkalinity Sample number Sample IP volume (mL) mL ml ml Imr. CaC03/L) MBIT .:..:.?;** Deiortized water, pi I 100 Jfe< io->3> -\S >>^ lQ\1 . lTU- £ti$k mur\ 2s 15.4 i^ il^iiuo^L tuo V£-iOi%.0\ X WL ^3-a H.o tk> \ 13lQ_ UHDCft.flt- dTetftenaayL, I 2.-S &L& MJl. Sm it) 2liL£^ U-iOU.cfb> 3&JL asa. Jc4_ it). _<i3o_ T +/-r l£ib>>*>. HO.*! tal .i2iKL t^tDio.n-& T//i tec oc>> zs 40: &g ilo loM fi aySgflMlHgjlg -1Ajxuq_-^£l I WiXkL SO O.C? .*?4_ j£3"^MM (t>cn3 15'O^arlJ? mtfsu? ttU loo fij *3 Jl JfllSTLdiL **.* >3>S' 5 J5X. 13J. 5.1 _§L £>TJ/4Squ in\ 5b B&. &uS- i*i£J.z) 54 JJ.-I0 lO-^> i o?l.S J4.3. 4* kkX 5_2_ 7F 1 kiorz.T-b tea as- ta 51.. AA-IOOg-CP- TM S(jk) in\ Lyj S 60 >Hr,1 4>>-U laj. 54-z At4 jjvj J5o_ LSafeBbad. i ^A iii. ^*tm-L-T-b- Iki *ii>_ JLk_li4 54 iiiaaiiJS hfyfr^to <K*T ! -58 fi>>A 33.<< U2l SM 5b tg" >>Ota>>*fi- 3ft.o 35_ jill 52 ..lrjjlG\^.-T-*-l S1.S" 111 r -* Laboratory control standard (LCS): ^ftsrftrefvc-e^tandacd True value (TV) Sample Begin End Total Multiplier Alkalinity (MV) % RS

~~MV / TV x 100 number (mgCStbi/'tT trcHonK? ml mL ml (mg CaCCh/L) (acceptable range = 90 to 110%)~~

(ml) INSS 100 100 ~6Su. io.i^s Reviewed by: tA" Date revhewed: 1&4H.W Page 58 of 91 SOP CS-Revision ^-Exhibit Cb.J 4fe f*fPC Page faf^**. Page K of 3, !**** Analyst e&u Alkalinity (SM 2320 B-2011) Date analyzed tcf&'rt Matrix: Water, RL -- 5.0 mg CaCOs/!., Samples are titrated to pH = 4.5 S.U. Titrant normality and muitipiier determination INF. Laboratory control standard fl.CS}: Reference standard True value (TV) Sample Begir End Total Multiplier Alkalinity (MV) %RS = MV/TVx 100

~~"Humbi?r (mg CaCOiA) volume mL ml ml. (mg CaCOs/L) (acceptable range = 90 to 110?i)~~

(ml.) INSS loo""---. --.. -i *'"* * *' Duplicate sample precision: Sample- AtkaHnlty 5JRPD Sample Sample ID volume Begin end Tcita4, , Multiplier (mg CaCOi/L) = {(S-O)/((S+D}/2j)xlQ0 number (ml.) mL mL mL (acceptable range i i 1054) s ^-^ ^__ ~"~~-es~ n Duplicate (D) C*lSnjJ Sample measurements: Sample Begin End Total Multiplier Alkalinity Sample number Sample ID volume (mL) mL mL ml (mg CaCOj/L) MC (TV i 2J;-mt7tf- -tJEionnexi'WilTfer, pH - su !

~~10 66^- !c.-v5-,ft, ItlDC^.O^ "TUaSQcO IkJTOJ \ 6b %.s IO.T. 3.T- (.z\ 10.4 5w~~
2. let >>."? <<*.S LV) l IfclBrfl .Vl~ \ <&

tjHtkX'L.fi 3 J- is?,** iS.l X.<L w GSL -^*-^ J i / / / s' X / c " OS* i>>n£ttg Laboratory control standard (LCS}: Reference standard True value (TV) Sample Bcgfn End Total Muitipiier Alkalinity (MV) KRSsMV/TVkIOO number (mg CaCOj/t.) volume mL ml ml (mg CaCO,/L) (acceptable range = 90 to 1105iJ (mL) INSS 11/3^ 100 100 \5.t #fc£ 1-3 10.4 opt *tfh&T* Reviewed by: It Date reviewed: IJOiiirill^ Page 59 of 91 SOP C6-Revision 4Exhibit C6.1

~~rgJEpp^~~

Page Analyst (^ Hardness (SM 2340 C-2011) Date analyzed Matrix: Water, RL= 5.0 mg CaC03/L ia Jfrfl Titrant normality and multiplier determination: Titrafit reference Normality check Begin End Total Normality (#) of EDTA pH Factor or Multiplier number standard number mL ml ml <<0.2/E i {# x 50000)/ 50 ml sample (acceptable range << 0,0180 - 0.0220) INR 0^>>4 INSS 112J& J2JL 10a) i o.o 0-0200 ZD'O Laboratory control standard (LCS): I Reference standard True value (TV) Sample Begin End Total Multiplier Hardness (MV) %KS=MV/TV*!00 number (mg CaCOs/L) volume ml mt ml (mg CaCO$/q (acceptable range =90to 110%) (ml) INSS R-Ol too Ho l 00.O7. 40 50 l2-o l^O too Duplicate sam pie precision: Sample Hardness %RPD Sample Sample 10 volume Begin End Total Multiplier (mgCaCOs/l) =((S-D)/KS+D)/2J)K100 number mL ml ml (acceptable range - 110%) a#-r\>F*rt 0D 17.0 K.% 0.2> 70,0 S U-O 1 \ D Duplicate (D) &> \7j& UOCTfttf Sample measurements: Sample Begin End Total Multiplier Hardness Sample number Sample ID volume (ml) mL ml mL {me CaCQs/L) MB{TV<2.Smg/U Deionlzed water 50 0.& 0-o o.o 26X3 >>x<<0 COh MP op I (7.V0 \T<e> 0.£- f 4.0 *^iO anerr &\ t2ft >>3. I 0 3 (4.0 to^fer UP foi >>3ju1 <0^ U'^l rcdt^ gb> ai Mt/>£}tf0 lb Ol n./ 3.V SO 3-*ft "i^ai^M J Ofl ^O il>i n.s o,u 60 i£-/6o4.o\ Oarnhtt-de *K 21.>> J,(, *te IO-DM0 6svO 41,1 <<3,l 2^0 MO j 0.02*16 1 Z$,l *£l 5*f> 40 fe-O^-av*.tefl mVBuj 2s,i au M.?. 0M I o n je>>& 1 flte 83.T M.i 6^1 kfe io.oi.ia 1 33.^ 3"?,} 4.2- Q^ 21 2^38^ >>. *r-"^ lM ^^~ ^<^1 Laboratory control standard (LCS): Reference standard True value (TV) Sample Begin End Total Multiplier Hardness %RS =WV/TVxiOO number (mgCaCO*/l) volume mt mt ml (mg CaCOa/l) (acceptable range = 90 to 110%) (ml) £o ?Ojd HO IOO.J?. -,Nss wo< 40 50 31> m Date reviewed: [ tk~crvi it T^ Reviewed by: Page 60 of 91 cr\n re r>~

~~r-^i STa ,---77 Page HO Page ' of _5l_~~

Analyst J&L. Hardness (SM 2340 C-2011) Date analyzed Matrix: Water, RL = 5.0 mg CaCOs/L \o- ajg Titrant normality and multiplier determination: Normality check Begin End Total Normality (W) of EDTA pH Factor or Multiplier Titrant reference number standard number ml mL mL = 0.2/0 - (W x 50000)/ 50 ml sample (E) (acceptable range = 0.0180 - 0.0220) rfVxlOOO O.O'Zoo Tjo. o INR Hf-rf £4+ INSS \\J\% CO lo.C1 IO.O BSBSgg Laboratory'controlstandard (LCS): Sample Begin Multiplier Hardness (MV) %RS = MV/TVxl00 Reference standard True value (TV) End Total (mg C3CO5/L) volume mL mL (mg CaCOs/l.) (acceptable range = 90 to 110% number (mL) INSS reK> 40 50 lo.o V2-< Z.o Z/O.o -~^Q SOO.O^fe Sample Hardness JSRPO Sample ID Begin End Total Multiplier (mg CaCOj/L) = {(S-D)/[(S+D)/2])xl00 Sample volume (acceptable range = +/- lOSi) number mVC (n.L) mL mL ml. EES.^oifloS 5o S2,.-d B.l \.1 Zo.o S ^4 7) Duplicate (D) X. 15.2 iHH >>. X J- >>?4- feSU 1° <<*!* Sample measurements: Sample Begin End Total Multiplier Hardness Sample number Sample ID volume (ml) mt mL mL (mg CaCOj/L) MB (TV< 2.5 mg/L) Deionized water 50 O .0 O. 0 O.o 2-o.t> PC Q5S**- ^Ql-6cu rn\/Li- 50 SS.-}- \-5 ?&>> f A -Of3 -/©A ^SuJ 1 16.1- 1-3-Lo 1-^ %s /7). IV6 . frfc* 1 i 11-.1* n.H l.*J 3u 1 D,Jl.t0 I W.H £?i.i L*? 34 H.o wo 11.11-104 /hAki/J c?i.l RS.l ./&)/. >>3iZ M-1 £5.1 *1.& H.i y/)W2^6 T 5<<^ 66.+ >4,2- <34 53.4- H.o £0 tojj^.te 37.4 /D-D S.ifi J 3?. 4 -41.+ 4.o fco i# lOOtf-O^ /%Vi/tU<xrj 5P *MA *4*+. 1 2.4 54 i&ioso.'bO z I *44.1 Hfe.S *# 54 l^JOlT'XI- 5 X 4i** *<<.*r 2.7 §4 U 10cA .o4 D)p>^ kk^JTP l 51) O.o 1.2- U'2. <<?4- . z ~J,2 3.4 1,5 ^Vo 1 ft / C> 1\ . QS ffcitn^.cf^ J ^ 1 °$&& W 1.2. l.7~ a<<_ &4 %.o l&ZOn^. QS? T/Viurv^lu? \ Sb 3t& C.4-0,.) i£rm\ \, in I 2 J-1 33 D.Z 4.0 1?. ltll"*. .cM J J 3i&^ .<< 1-3 0.1 $.b UlOCft.oW lk*YHjbb 1 5U 3.? 4-.I t>J \\e . Laboratory control standard (LCS): %RS = MV/TVxl00 (acceptably range = 90 to 110%) Page 61 of 91 SOP ce-Revision 4-txhibit C6.2

Sfsa irr-3 ,-v

? Page 4Q Page. 2* of_a_

Analyst Q&\. Hardness (SM 2340 C-2011) Date analyzed lo-v3-i% Matrix: Water, RL = 5.0 mg CaCOj/L Titrant normality and multiplier determination: TTfrsmrefaasnce Normality check Eegin End Total Normality (<<) of EDTA pH Factor cr Multiplier number ' Standardjuimber_ mL mL mL = 0.2/E = (N x SOCOO)/ SO mL sample (ET~ (acceptable range * (JillUU-UJJ<<<<af~ = <<xl000 iNR INSS

~~e<<5i- i^iS'xf, Laboratory control standard (LCS):~~

~~Reference standard True value (TV) Sample Begin End Total Multiplier Hardness (MV) %RS~~

~~MV / TV x 100 number (mg CaCOs/L) volume ml. ml ml (mg CaC03/L) (acceptable range = 90 to 11054)~~

(mL) imss aoi 40 SO M-.l tp.l <?.o 7o,v 4-d IOO. 0% Duplicate sample precision: Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOa/L) = ((S-D)/[(S+D)/2]}xlOO number (mL) mL ml mL (acceptable range = +/-1054) l*!Bll<<0l" ^*(jur^itt 7- m Ut.\ U-M o.s 2-0.0 )U> i D Dupiicate (D) X UA 7.7 D/3 X esc **>>***!<< Sample measurements: Sample Begin End lotal Multiplier Hardness Sample number Sample ID volume (mL) ml mL mL (mg CaCOj/L) JU £Si_ Itr-iinl i £tt>\*.o& iWfVK* 7T- 3 eo 1.3 -7£ D7 "Z^O-O IS VV ioo4-dI \Z^W<^jj-\ J 10 7.7 ID.?- 3.1 ( SO 1 3io V fc to.4CT \ 2 J,o/S Z.% I o\ 3so J- SS^We-i 14 .-It \ilV)V^.O\ J h 4.2 .o 32° ^ £ ro &K.G7- Lh^^anoo^^ 1 2s I4.U >><<<< *+,X- ( -2.1 170 iRj&n.Gib t ^* 1 is.s *3.1 H-.4 -*> 17-0 3 J_ k.4 4.a itiDi&.tn- to.u- **> i-3-d 1 t /ftin.aS" ~f\M UC Of) I 2S .33. i 377 4.tj> *> \?o -i&a 1k i o I o. nP\. i \jjjtMJ <5D ^1.7 S3.S I.S 3U i o- trs-i 4 mH-iuJ VY I 5D (3*1.5 33. to Hi 82. lD-in-i ^ , 2 l 33.to 37. te H-.o eo ln-i\-ifc fN J 3 1 371* *41. U H.o So l£ 1r-\Cv*-0>l, -h//A SGuO iiij I 5o 44.1* 44.3 5.7 54 44.3 HieM a.s 5o \g 1 Ci iQ.^ 1 z i J 3 1 19, IDlT.T-'i 40. .'6 4^.5 -?.7 54- \ %IO OR.JPS?) HVA bGlto IQl ^^ 1 6b IO.L* i3.5 "3.7- 54 ifrlD lO^j* \ I j B.3 l5.ffi ^.Lp 5A \ <4 I 0 11.. TA J 3 _L 15.1 ^ J.7 <<* J-J

~~10.:~~

vk io OS*<<S "^kSSuO !£eiK nst 1 <<* l&fc 52 Labp^{vty<<uztjvlstandard (LCS}: Page 62 of 91 SOP C6-Revision 4-Exhibit C6.1 Page '^ c Page. I of g. Analyst SSl- Hardness (SM 2340 C-2011) Date analyzed lo.l^i^ Matrix: Water, RL - 5.0 mg CaCOj/L Titrant normality and multiplier determination: Titrarrt-rcterenee. -&orrna?ity check Begin fnci Total Normality (A/)of EOTA pH Facicr or Multiplier number standard number ..... =0.2/E = (/V x S0000)/ 50 ml sample (a7cepTaW?raTIj^^>><MUafi-0.()220) = /VxlO0O INR INSS 6Si_ io'<<3">£ Laboratory control standard (LCS): Reference standard True Vrilue (TV) Sample Begin F:ncl Total Multiplier Hardness (mv) K,RS = MV/TVxl00 number {rng CaCOj/L) volume mL ml mL [rag CaCOj/L) (acceptable ranne = 90 tc llo;;) (mL) INSS sM v"*?t i 40 50 eKS" 33.ff l.a "t*& ,o *Vo lOD.O°L' Duplicate sample precision: Sample Hardness 5SRPD Sample Sample ID ite^in End Total Multiplier (mg CaCQj/l) = {(S-0)/([S+D)/2]}xlO£l number 1^4 (ml) mL ml mL (acceptable range = +/- 10 >] 2-0.0 55. i £/nit> .VI- SGixHwr Z 50 5s.r act <a.u> 0 \ Duplicate (D) X atf..i ^.7 =?.u- -X 3"?s PgL l>=-(5,.^' Sample measurements: Sample Begin End Total Multiplier Hardness Sample number Sample ID voiume (mL) mL mL mL (mg CaCOs/L) MB (TV c2.-7Jiiit,/L)

~~Dciur.trtrd-tVdtyr 50 8so(o*i3-t%~~

l£ ioi-l.. T.^ <\l A S(SW 1 MT 3 so Stt.7 ai.4 4.7 *2-0.0 54 it icvn^. CJS IVAftoiJ (^T0n> 1 "*3D 71.4 3M-.3 J(.n \ 5* L 34.3 3V4 AM 5a 1 £10 i4>tW 1 l£ io\1-T>\ 3 1 3V-*J &<<Mr 5.7 54 ~-~~^ 1 ^ ~~ ^x- ^x"" 3* y s 1 ~v_ ~ ftS<_. lo.i3-tR Laboratory control standard (LCS): Reference Standard True value (TV) Sample Begin End Total Multiplier Hardness X RS - MV / TV X 100 number (mg CnCOj/l) volume mL mL ml (tag CaCOi/L) (acceptable range = 90 to 110K] (ml) INSS fttf 40 50 3<U HSX 1.1 Tfi.o S*o <i$0 *k Reviewed by: <A~ Date reviewed: [ l0,t<<4t# Page 63 of 91 SOP C6-Revision 4--Exhibit < Seqwyafe Mwleax Plant Biomonitormg Appendix D Reference Toxicant Test and Control Chart Page 64 of 91 t-T> if 3!*V~rf-Pimephales promelas Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc. Source: In-house Culture 1.2 i r l 1 1 1 1 1 1 1 1 i i i r Control Limits (+/- 2 Standard Deviations) 1.0 0.8 O sM^.r 0 0 O © 0.6 - 0.4 bO J L i 1 I I L J I J* 1-2 i 1 1 1 1 1 1 Warning Limits

~~o 1.0 -~~

~~i*>^^>>V5<^^>>>>^<^^~~

Test date 7-day IC25 = 25% inhibition concentration. An estimation of the potassium chloride concentration which would cause a 25% reduction in Pimephales growth (calculated using ToxCalc). Central Tendency (mean logarithmic IC25 converted to anti-logarithmic values) Control Limits (mean logarithmic IC2S +/-2 standard deviations converted to anti-logarithmic values) Laboratory Warning Limits (mean logarithmic IC,5 +/- 2 coefficent of variations convertedto anti-logarithmic values) USEPA Warning Limits (mean logarithmic IC25 +SA 75 converted to anti-logarithmic values, SA 75 =75lh percentile ofCVs reported nationally by USEPA) Mm Sumnet Page 65 of 91 Pimephales promelas Chronic Reference Toxicant Control Chart TO tnVJronnwital Testing Srtlutlam, Int. Source: In-house Culture o Logja Conversion AntMogarithmic Values (g/L KCf) ^§t number Test date 7-day IC25 Laboratory Calculated CV 75th Percentile CV ToxCal Determination 7-day ICM a S CT Control Limits Warning Limits Warning Limits (g/L KCl) CT-2S CT + 2S CT-2CV CT + 2CV CT-SA,75 CT+SA.75 1 05-02-17 0.6740 -0.1714 -0.1371 0.0316 0.7292 0.6305 0.8434 0,5939 0.8858 0.4521 1.0063 2 05-16-17 0.6906 -0.1608 -0.1382 0.0320 0.7275 0.6278 0.8431 0.5904 0.8864 0.4511 1,0040 3 06-06-17 0.7367 -0,1327 -0.1393 0.0314 0.7256 0.6280 0.8384 0.5911 0.8810 0.4499 1.0013 4 07-11-17 0.7519 -0.1238 -0.1393 0.0314 07257 0.6280 0.8385 0.5911 0.8811 0.4499 1.0014 5 08-08-17 0.6829 -0.1656 -0.1379 0.0294 0.7280 0.6357 0.8336 0.6012 0.8731 0.4513 1.0046 6 09-12-17 0.7271 -0.1384 -0.1371 0.0292 0.7294 0.6377 0.8342 0.6037 0.8730 0.4522 1.0065 7 10-03-17 0.7024 -0.1534 -0.1382 0.0293 0.7274 0.6355 0,8326 0.6011 0.8720 0.4510 1.0038 S 11-07-17 0.6666 -0.1761 -0.1421 0.0289 0.7210 0.6310 0.8238 0.5961 0.8636 0.4470 0.9949 9 11-07-17 0.6847 -0.1645 -0.1404 0.0265 0.7237 0.6406 0.8175 0,6089 0.8533 0.4487 0.9987 10 12-05-17 0.7191 -0.1432 -0.1424 0.0251 0.7205 0.6418 0.8089 0.6112 0.8432 0.4467 0.9943 11 01-09-18 0.7574 -0.1207 -0.1397 0.0244 0.7249 0.6478 0.8113 0.6185 0.8441 0.4495 1,0004 12 02-06-18 0.7951 -0.0996 -0.1377 0.0260 0.7283 0.6461 0.8211 0.6154 0.8557 0,4516 1.0051 13 03-06-18 0.7002 -0.1548 -0.1387 0.0263 0.7266 0.6437 0.8201 0.6126 0.8552 0.4505 1.0027 14 04-03-18 0.6973 -0.1566 -0.1411 0,0256 0.7225 0.6423 0.8128 0.6115 0.8474 0.4480 0.9971 15 05-08-18 0.7204 -0.1424 -0.1429 0.0243 0.7196 0.6435 0.8048 0.6138 0.8380 0.4462 0.9931 16 06-05-18 0.7512 -0.1243 -0.1451 0.0200 07160 0.6530 0.7852 0.6280 0.8126 0,4440 0.9881 17 07-10-18 0.7808 -0.1075 -0.1429 0.0216 0.7196 0.6514 07950 0.6248 0.8244 0.4462 0.9931 18 08-07-18 0.7690 -0.1141 0.1410 0.0225 07228 0.6518 0.8015 0.6245 0.8317 0.4481 0.9974 19 09-11-18 0.6884 -0.1621 -0.1432 0.0222 07191 0.6491 07967 0.6217 0.8270 0.4458 0,9924 20 10-09-18 0.6735 -0.1717 -0.1442 0.0231 07175 0.6452 07979 0.6167 0.8296 0.4448 0.9901 Note: 7-day IC2S =25% Inhibition concentration. An estimation ofthe potassium chloride concentration that would cause a25% reduaion in Pimephales growth (calculated using ToxCalc). CT>> central tendency of the IC3S values. S = standard deviation of the iCj5values. Control Limits = Mean logarithmic IC +/- 2 standard deviations converted to anti-logarithmic values. Warning Limits

~~Meanlogarithmic IC2S t 2CV or sA.<<, convertedto anti-logarithmic values.~~

SA.,S ~Standard deviation corresponding tothe75"' percentile ofCVsteported natiorraliy by USEPA (5A75 *0.38). CV = Coefficient or variation. r4/rt>t\trtv4 m*a f = tmlnwuwwiut T.tttng Sekritoni, tec Pimephales promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture 1.25 . I 1 1 i 1 - 1?Q) 1.00 o JC CO

~~M *>~~

-->> Q .C-O c *

~~+->> 4 0.50 C -^ o bfl u E -~~

~~=* 0.25 USEPA Minimum Acceptance Criteria (> 0.25 mg per surviving larvae) -~~

1 1 1 1 i i i i 1 1 >> I I 1 i 1 1 1 I 1 - ir iiiiiiii i i i i iT USEPA Maximum Coefficient of Variation Guidance Criteria (< 20%) 20 II .5 o w 5 £0 i*2 10 0 o c o 01 u 2 H= J; O - ©**-> *>> <u o 0 u J L I I I I I I _L J I 1 1 1 1 1 1 1 1 1 1 I I I 1Ir USEPA Upper PMSD Bound Acceptance Criteria (< 30%) 30 20 Q CL 10

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~~/$ A<<A1 <<firtf *v*1 <<ftAn *-lAn *fl rfl*1 *& <*A1 *r& && <*& -A* ^ &<$> Ktf8> ,*& kk& -*&~~
Test date Control Growth, Coefficient of Variation (CV) or Percent Minimum Significant Difference (PMSD) PMSD is the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is not a minimum acceptance criteria. £nip<<*dand Central Tendency (mean Control Growth, CVor PMSD) Reviewed by Urn Sumner 95% Confidence Interval (mean Control Growth, CV or PMSD +/- 2 Standard Deviations) Page 67 of 91 Pimephales promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria litttormuMtHmtrq tgtatMm,t>> Source: In-house Culture s-4 ToxCal Determination Control Growth Control Growth CV Test PMSD Control Control Growth Test (mg/lnltiai larvae) (%) Test number Test date Survival Mean CV MSD PMSD a 95% Confidence Interval CT 95% Confidence interval a 95% Confidence interval (%) (mg/initial larvae) i<<) (%) CT-2S CT + 2S CT-25 CT+2S CT-2S CT + 2S 1 05-02-17 100 0.729 5.6 0.0792 10.9 0.596 0.416 0.776 6.2 0,6 11.8 11.4 5.0 17.8 2 05-16-17 100 0.593 2.8 0.0521 8.8 0.603 0.434 0.771 6.0 0,2 11.8 11.4 5.0 17.8 3 06-06-17 100 0.608 7.2 0.0542 8.9 0.601 0,434 0.768 5.8 07 10.8 11.0 5.2 16.8 4 07*11-17 100 0.713 3.3 0,0589 8.3 0.609 0.438 0.781 5.6 0.5 10.7 10.8 4S 16.8 S 08-08-17 100 0.638 7.9 0.0791 12.4 0.616 0.451 0.781 5.9 1.0 10.8 11.0 5.1 16.9 6 09-12-17 100 0.631 5.6 0.0562 8.9 0,626 0.489 0.764 5.9 1,0 10.8 10.8 5.0 16.5 7 10-03-17 100 0.658 4.4 0,0838 12.7 0.637 0.525 0J48 6.0 1.2 10.7 11.0 5.4 16.6 8 11-07-17 100 0.738 3.9 0.0641 8.7 0.645 0.528 0.761 5-7 1.1 10.4 10.8 5.2 16.4 9 11-07-17 100 0.727 0.8 0.0580 8.0 0.642 0.535 0.749 5.3 0.6 10.0 10.5 5.0 16.1 10 12-05-17 100 0.676 2.3 0.0607 9.0 0.647 0.545 0,750 5.2 0,4 10,0 10.6 5.0 16.1 11 01-09-18 100 0,612 8.3 0.0752 12.3 0.643 0.543 0.744 5.4 0.5 10.4 10.8 5.0 16.6 12 02-06-18 100 0.772 8.4 0.081S 10.6 0.651 0.538 0 765 5.6 0.5 10.7 10.6 5.0 16.2 13 03-06-18 100 0.810 4.7 0,0640 7.9 0.660 0.526 0.794 5.6 0.5 10.7 10.6 4.9 16.3 14 04-03-18 100 0.743 6.1 0.0836 11.3 0.668 0.534 0.802 5.8 0.8 10.7 10.7 5.1 16.3 15 05-08-18 100 0.576 4.9 0.0516 9.0 0.663 0.523 0.803 5.6 0.7 10.5 10.8 S3 16.3 16 06-05-18 100 0.643 8.7 0.0992 15.4 0.664 0.525 0.803 5.8 0.7 10.9 11.2 4.8 17.6 17 07-10-18 100 0.678 3.9 0.0850 12.5 0.669 0.535 0.803 5.6 0.5 10.7 11.3 4.9 17.7 18 08-07-18 100 0.692 6.4 0.0813 11.7 0.675 0.547 0.802 53 1.0 9.7 10.9 6.0 15.8 19 09-11-18 100 0.709 8.4 0.0680 9.6 0.678 0.551 0.805 5.5. 0.9 10.0 10.9 6.0 15.8 20 10-09-18 100 0.973 6.8 0.0693 7.1 0.696 0.516 0.876 5.5 i.0 10.1 10.6 5.5 15.7 Note: Control Survival = USEPAminimum test acceptability criteria 2 80% survtvai Control Mean Growth s USEPA minimum test acceptability criteria ^ 0.25 mg/su>>viving larvae. CV* Coefficient of variation for control growth. USEPA maximum CV guidance criteria (90th percentile) < 20% MSD = Minimum significant difference. PMSDs Percent minimum significant difference. PMSD isa measure oftest precision. The PMSD isthe minimum percentdifference between the control andtreatment that canbedeclared statistically significant Inawholeeffluent toxicity test Lower PMSD bound determined byUSEPA (10,h percentile) >12%. The lower PMSD bound represents a practical limitto the sensitivityof the test method and is not a minimumacceptance criteria. Upper PMSD bound acceptance criteria determined byUSEPA (90,H percentile) <30%. CT* Central tendency.of the growth, CVor PMS0values. S = Standard devotion of the growth, CVor PMSD values Page I of6 M ' Environmental Testing Solution!, inc. Potassium Chloride Chronic Reference Toxicant Test (EPA-822-R-02-013 Method 1000.0) Species: Pimephales promelas PpKCICR Test Number: 44 Dilution preparation information: Comments: KCl Stock INSS number: inss nv\ Stock preparation: 50 g KCI/L: Dissolve 50 g KCl in i-L Milli-Q water. Dilution prep(mg/L) 300 450 600 750 900 1050 Stock volume (mL) 9 13.5 IS 22.5 27 31.5 Diluent volume (mL) 1491 1486.5 1482 1477.5 1473 1468.5 Total volume (mL) 1500 1500 1500 1500 1500 1500 Test organism information: Test information: Organism source: In-house culture Randomizing template: ^OlUuJ Age: < 24-hours old Incubator number and shelf location: n6 Spawn date: ua-tA-*fc Ariemia CHM number: CHM984 Hatch dates and times: to-o&-i£ l^x<* *.** Drying information for weight determination: Transfer vessel infonnation: pH= -\,SO S.U. Date / Time in oven: /D-ifc-tf tftMS Temperature<< ~V\A °C Initial oven temperature: feO'C Average transfer volume: < 0.25 mL Date / Time out of oven: ftm-tr crv4£> Final oven temperature: tVC Total drying time: -L+riouitb Dailyfeeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW or termination bntch used Time Analyst Time Analyst Time Analyst 10-09-18 OlO^ 4 ITOS S- b&lU -Jr-iO-dVI^ 10-10-18 Jt- \XGfc O&lO n-(sb-i<t OlQft 4-10-11-18 eho A- H.ID 4L olfu & /o-evtf 4 4 10-12-18 ftV>>lQ n.ib QglO u-<n-il -£* +/- ID-\WfcA 10-13-18 CAoO CHbO i^DO -&r *it 10-14-18 enco 4i- V^OQ -ft- CA-tt Ifr-iVifrft 10-15-18 \2.\o nenfc "ft >il-if A.
f **
fitoOO 10-16-18 pin i Control information: Acceplance criteria Summary oftest endpoinis: % Mortality: C>7. <20% 7-day LCso <§2G. NOEC 4SO Average weight per surviving larvae: OA-Xb > 0.25 mg/Iarvae LOEC <o0G ChV Sl<<.(* ICis ^VS SOP AT21 - Exhibit AT21.1, revision I l-Ol-M Page 69 of 91 ^stm Page 2 of6 EnvlronrnemalTeiWftjScttKion*,Inc. Species: Pimephales promelas PpKCICR Test Number: 44 Survival and Growth Data Day 1 Control 300 mg KC1/L 450 mg KCI/L A B c D E F G H I j K L 0 10 10 10 10 10 10 10 10 10 10 10 10 1 /O fO '0 1^ '0 10 >>o 10 10 10 *0 10 2 /o /o. JO /o <<0 '0 '0 '0 >>0 <<0 <0 '0 3 '0 '0 10 ID 10 Ifc 10 <0 fO >D 10 '0 4 /o '0 '0 '0 10 10 /o 10 '0 to f 0 >0 5 /o (0 /o JO /o 'o CO '0 '0 /0 to 10 6 /O 10 /o /O 10 10 ib 10 /D t0 10 to 7 /O /o 10 /o 10 '0 '0 '0 'o /o <0 '0 A - Pan weight (mg) Traycolor code:: QCl^f Analyst: TS /3.W I4.<?a I3.<& ft# £07 13.40 M./o IW aai 13.?1 /3.#l 13/fc Date: Cl-A^ll? B = Pan + Larvae wcieht (mg) Analyst: \A Tb.Qb Ti.l<? i>>,.it l^Sb w/u t&A* -a/tf *Ut-3b Tl.^ ivan rval TZ.&b Date: to-Wi£ C = Larvae weight (mg)=B - A I In ltd calculated. , ^.0*8 iO.SU <\A^ <\.<<>> <\.&4 V*8 1.a<\ H-H-l <<V11 *S.su 16. IS ^.t.T Analyst: V\ Weight per initial number of larvae (mg) = Cl Initial number of larvae A. A. ^ JSP Hand calculated. . Analyst: Y\ 0' 0' tf 0" 0' Average weight per initial Percent number of larvae (mg) reduction O.W? o.^uS o.S.7. O.Vrt <>>7. from control (%) ,'^l,;;.'-: f--.,j.;.. Comment codes: c = clear, d = dead, fg = fungus, k - killed, m ~ missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Conmients: SOP AT21 - Exliibit AT21.1, revision 11-0 i-14 Page 70 of 91 Page 3 of6 _* EnvironmentalTesting Sch>>tiw<<. Inc. Species: Pimephales promelas PpKCICR Test Number: 44 Survival and Growth Data Day 600 mj KCI/L 750 mg KCI/L 900 mg KCI/L M N O p Q R s T U V w X 0 10 10 10 10 10 10 10 10 10 10 10 10 1 to fO IO to '-0 fD to to g* s1* T* SM 2 tO >>0 IO 10 to to to to & & n S 3 (0 to 10 to ID IO fO <<Cs <£ £ n 8 4 'D *0 JO <0 10 V* 10 V< -i* ^ i V41 5 10 <<* <<0 V* S^ &l1 1 sM sa 5* 3> s 4* 6 IO <\ 10 °l S ^ "V* S *> *s q 7 ^*4. <^<<\ ^ 4"t 4 \ 1 Y* U> C n "b A = Pan weight (mg) Trav color code:: 3*^s/ Analyst: "TS v IH.05 13.74 I3.3H Mb &W 0.31 D.M0 K?H £# mo M.tf £74 Date: Oq.2MAV B = Pan + Larvae weight (mg) Analyst: ll.T^ 2VUo *&.*& n . n 2T,tb zv.'to PU\ "IV1& H.Sl U.M atf 20.40 Date: C = Larvae weight (mg) =* B - A Hand calculated. , £.>i S.Xl %M i.M C.T8 u.& s.H fc.V* 4.ofc. *2/W M.vl M.Ufe Analyst: J[ Weight per initial number of larvae (mg) = C / Initial number of larvae Hand calculated. > NO <** Analyst: V"\ 0' o* ° 1 0" 0' 0' Average weight per initial Percent number of larvae (mg) reduction 0.8 ^4 \<,.V7. 0.W5/1- 3S.l7. CVU s<*. \ 7. from control <%) Comment codes: c = clear, d = dead, fg = fungus, k - killed, m - missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Comments: SOPAT21 - Exhibit AT21.I, revision il-Ol-14 Page 71 of 91 "jGT* Page 4 of6 _. EnvJronmtoUJTciUngSc!o<<tent,Inc. Species: Pimephales promelas PpKCICR Test Number: 44 Survival and Growth Data Day 1050 m gKCl/L Y z AA CB 0 10 10 10 10 \\\pA> s5* 5^ SsA 1 2 <A S S s 3 ^ S S s
b*
4 5 4 .4 \ 6
x. \ T_ T_
l'* 1* 7 z \ A << Pan weight (mg) \5.lb m.oo W.7I IH.57 Tray color code:: cifeW Analyst: fS ° Date: OI'^'J* B = Pan + Larvae weight (mg) Analyst: l( n.is vk>s\ isnt js.v>>i Date: /0*n-t£ C = Larvae weight (mg)
B - A Hand calculated. . iM o.&<<\ t.oi LOS Analyst: V\
0 ' Weight per initial number of larvae (mg) - C / Initial number of larvae ^5 Hand calculated. Analyst: jr\ o" Average weight per inltiu] Percent number of larvae (mg) reduction from control o . \ TS <si.n. (%) Comment codes: c = clear, d = dead, fg - fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r - decanted and returned, w = wounded. Comments: SOP AT21 -ExhibitAT21.1,revision H-Ol-14 Page 72 of 91 Pimephales promelas Chronic Reference Toxicant Test EPA*821-R-02-013, Method 1000,0 F.miromncnUl TpMng Setuitoni, Inc. Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: Test dates: October 09-1$, 2018 CorwmlraiHiFt fmj/l KO) R<<(i1{faie inttbiflumtwof final number of A
Pm welfht (m<<) 0 a Pan t Urw>># Uivas weight Jwgl

Weight / Survives Mean unsigHf/ttmrivfot Gtxefffcient of variation M<<*n turtfcal (H) M<<*Aw#lcht/Mllte> Cofiffttfent ot vaibiton P<<rwnt reduction from larvae laivae 0-A number 61 larvae (ml) numbfff cH<<fv*e (mg} Urvae lrnjj number at i>>rv>># m contra! (Nl
<<vmt>>t4lwn<<<<J (iJJ fml> A 10 10 13.98 23.06 9.08 Q.90S 0.908 B 10 10 12.62 23.13 10.56 1.056 1.056 Control 0.973 6.8 100.0 0,973 6.8 Not applicable C 10 10 13.22 33,16 934 0.994 0.994 D 10 10 15.23 24.56 9,33 0,933 0933 E 10 10 15.07 24.91 9,84 0984 0.984 F 10 10 13.40 23.38 9.98 0.S9S 0.998 300 0.965 3.3 100.0 0.965 3.3 0.8 6 10 10 14.10 23.39 9.29 0.929 0.929 H 10 10 14.89 24.36 9.47 0J47 0.947 J 10 10 13.21 22.98 9.77 0.977 0.977 1 10 10 13,81 233? 9.56 0.956 0.956 450 0.979 2.6 100.0 0.979 2,6 -0.6 K 10 10 1-3.24 2339 1045 1.015 1.015 I 10 10 13.15 22.83 9.67 0.967 0.967 M 10 9 14,05 22.79 8.74 0.971 0.874 N 10 9 13.74 21.96 8.22 0.913 0822 600 0.915 43 90.0 0.824 4.3 153 0 10 9 13.34 21.33 B.04 0.893 0.804 P 10 9 14.92 22,87 7.94 0.882 0.794 Q 10 7 15,88 22.66 6.78 0.969 0.678 R 10 6 15.31 21.70 6.39 1J065 0.639 750 0975 6.6 65.0 0.632 7.0 35.1 S 10 6 13.40 19.11 5.71 0,952 0.571 T 10 7 15.84 22.23 639 0.913 0.639 U 10 4 15.45 19.51 4.06 LOIS 0.406 V 10 3 13.40 16.34 2.94 0980 0 294 900 1055 7.6 37.5 0.39S 18.5 59.1 w 10 4 14.9S 19.19 4.24 1.060 0,424 X 10 4 15.74 20.40 4.6S 1465 0.466 Y 10 2 15.16 1745 i.98 0.995 0.199 z 10 1 14.00 14.89 0.39 0.890 0.089 10S0 1.001 8.1 12.5 0,125 40.0 87.1 AA 10 1 14.71 15.78 1.07 1.070 0.107 BB 10 1 14.57 15.62 1.05 1.050 0.105 Bunnell's MSD value: 0.0693 MSD* Minimum Significant Difference PMSD: _74 PM5D- PercentMinimum Significant Difference PM5Dis a measure of test precision. ThePMSD ii the minimumpercent difference between the control and treatment that can bo declared *jtatijticaliy vgmficant In9 Wtsole effluent toxicitytest. Lower PMSD bound determined by U5EPA(iQth percentile)
12%
Upper PMSD bound determined by USEPA (90th percentile)<< 30H. lower and uppar PMSD bounds vvare determined from the 10th and 90th percentile, respectively, of PMSD data from IPA'sWET fmerlabcratory Variability Study{USEPA* 2001a; USEPA, 2001b). The lower PMSDbound represents a practical limit to the sensitivity of the test method and 12 not a minimum acceptance criteria Ig^o *"^*"J**>> ^ -c Statistical Analyses Ei^rotm>entarTfe£tl>>9 Sotutl<<ft>>, inc. Larval Fish Growth and Survival Test-7 Day Survival Start Date: 10/9/2018 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 10/15/2018 Lab ID. ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date; Protocol RA/CHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mg/L 1 D-Control 1:0000 1 0000 1.0000 10000 30Q 1.0000 1.0000 1 0000 1 000Q 450 1.0000 1.0000 1.0000 1 0000 600 0.9000 0.9000 0,9000 0.9000 750 0.7000 0.6000 0.6000 0.7000 900 0.4000 0.3000 0,4000 04000 1050 0.2000 0.1000 0.1000 0.1000 Transform: Afcsin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-Mean Mean Mm Max CV% Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1,4120 0.000 0 40 300 1.0000 1.0000 1.4120 14120 1.4120 0,000 18.00 10.00 0 40 450 1,0000 1,0000 1.4120 1.4120 1,4120 0 000 18.00 10.00 0 40
600 0.9000 0.8000 1.2490 1.2490 1.2490 0000 10.00 10.00 4 40 T50 0.6500 0.6500 0.9386 0.8861 0.9912 6 464 1000 10.00 14 40

900 0,3750 0 3750 0.6584 0.5796 0.6847 7 979 1000 10.00 25 40

1050 0.1250 0 1250 0.3572 0.3218 0.4636 19.861 1000 10.00 35 40 Auxiliary Tests Statistic Critical Shew Kurt Shapiro-Wilk's Test Indicates non-normal distribution (p - =0.01) 0.86556 0.896 0.55749 2.4279 Equality of variance cannot be confirmed Hypothesis Test (1-tail, 0.OS) NOEC LOEC ChV TU Steers Many-One Rank Test 450 600 519.615 Treatments vs D-Control Maximum Likelihood-Probit Parameter Value SE 95% Fiducial Limits Control Chi-Sq Critical P-value Mu Sigma Iter Slope 10.0802 1.29616 7.53969 12,6206 0.54481 9.48773 0.96899 2.91423 0.0992 intercept *24.376 3.77401 -31 773 -16.979 TSCR Point Probits mg/L 95% Fiducial Limits EC01 2.674 482,445 400.783 541.007 EC05 3.355 563.709 491,749 614 83 EC1Q 3.718 612.486 547.704 659.058 EC15 3.964 647.759 588.463 691.326 EC20 4.158 677.235 622.492 718.692 EC25 4.326 703.59 652.705 743.651 EC40 4.747 774.639 731.584 614.793 EC50 5.000 820.791 779.454 865.351 EC60 5.253 869.692 826.7 923.242 EC75 5.674 957.514 904.335 1036,48 ECHO 5.842 994.776 935.374 1087.21 EC85 6 036 1040.04 972.077 1150.46 EC90 6.282 1099.94 1019.38 1236.43 EC95 6.645 1195.11 1092.42 1377.49 100 1000 10000 EC99 7,326 1396.42 1241.18 1690.55 Dose mg/L Dose-Response Plot
£ Page 74 of 91 Statistical Analyses
rrvrt'twTT>>f>>U)T<<i)f>5Soiu<*wi>>.tn<
Larval Fish Growth and Survival Test-7 Day Growth Start Date. 10/9/2018 Test ID- PpKCICR Sample ID. REF-Ref Toxicant End Date: 10/16/2018 Lab ID: ETS-Envir. Testing Soi. Sample Type KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mg/L D-Control 0.9080 1.0550 0.9940 0.9330 300 0.9840 0.9980 0.9290 0,9470 450 09770 0.9560 1.0150 0,9670 600 0.8740 0.8220 0.8040 0.7940 750 0.6780 0.6390 0 5710 0,6390 900 0.4060 0.2940 0.4240 0 4660 1050 0.1990 0.0890 0.1070 0 1050 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% t-Stat Critical MSD Mean N-Mean D-Control 0.9728 1.0000 0 9728 0 9080 1,0560 5.807 0.9728 1 0000 300 0.9645 0.9915 0.9645 0.9290 0.9980 3.316 0.259 2.180 0.0693 0.9716 0.9988 450 0.9788 1,0062 0,9788 0.9560 1,0150 2.620 -0 189 2.180 0.0693 0.9716 0.9988 600 0.8235 0.8466 0.3235 0 7940 0 8740 4.324 0.8235 0.8466 750 0.631 B 0.6494 0,6318 0 5710 0.6780 7.040 0.6318 0.6494 800 0.3975 0.4086 0.3975 0.2940 0.4660 18.475 0.3975 0.4086 1050 0.1250 0V1285 0 1250 0.0890 0.1990 39.989 0.1250 0 1285 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97485 0.805 0.45632 0.19391 BarttetTs Test indicates equal variances (p = 0.26) 2,69204 921035 Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob dt Dunnett's Test 450 >450 0.06931 0.07125 0.0002 0.00202 0.9047 2.9 Treatments vs D-Control Linear interpolation (200 Resamples) Point mg/L SD 95% CL<Exp) Skew IC05 498.11 64,08 68.37 523.00 -3.1814 IC10 547.37 22.37 449.63 595.31 -0.6658 IC15 596.62 21.74 503.54 637.47 -0.7512 IC20 635.44 17.65 561.63 667.88 -0.8234 IC25 673 48 16.69 607.02 708.82 -0.5824 IC40 780.80 14.84 720.53 813.59 -0.5453 IC50 843.09 14.63 793.67 881.03 -0.0458 500 1000 Dose mg/L Dose-Response Plot 1-tail. 005 level of significance Page 75 of 91 PEHE Page 5 of 6 ' imimnmtM>>\TetSagSaUnisni,lac. Species: Pimephales promelas PpKCICR Test Number: 44 Daily Chemistry: Day (Analyst identified foreachday, performed pH>> D.O.and conductivity measurements only.) 0 1 2 Analyst HT A*/ *41 TS T<<, *0 Concentration Parameter ^V"!':^ IH^C":"^'1 ssijisi ESIffi? pH(S.U.) *7.<2>><? ^=75" "7-7^ 1.S5 7.5? ^.33 DO (mg/L) **? ^rw ^e 7.1 £0 Xio Conductivity (^mhos/cm) 3h Wi~^m 3>/C 3c& CONTROL ^Alkalinity (mgCaCG3/L) ^Hardness uo ^ * ^ ' -~ . V^*K s<\ j^^Vr%^^ (mgCaCOs/L) s4 50 ^Temperature (°C) TA-Va t\-H of\-S -\\-h ;Vfc"\ Xv\.^ pH(S.U.) 7-fi ?.?*/ 1j99 TSCo 7.96 %i^i DO (mg/L) ^.<? ^Y &o 7.1 ?,0 4.^ 300 nig KCI/L Conductivity (umhos/cm) #s*r r ' j * >M' * #?? :'";:.;l;^; ;j! 935 ^Temperature (°C) TM-fc *v\-s iM.i T-vl-S TA-W *V\-^ pH (S.U.) *=?.m "3.^3 "^.fcs- 75.2 *79^ a.1^. DO (mg/L) %8 1.H &o 9.0 9./ br\ 450 mg KCI/L Conductivity T:SI,r t^ "I" ^>jr*" I " r (umhos/cm) )\ZO IIZO /£#>
Temperature (°C) -\s\-V>> -VJA-S -V\A -wt.s TA-W -u\.<i pH (S.U.) ^.£7 ^7*T ?.<?<Y "7"jS 79.2 an-DO (mg/L) 7.*? ^S SM 2.SL *./ ^0 600 mg KCI/L Conductivity (umhos/cm) /37* '., --.*;. ^---r' 1310 yPi^%-£ /353
^Temperature (°C) T.HA. -lAT -mKA "VA-lo 1>U -vA-i pH(S.U) "?-#* ^M ^TZ 759 7.91 'M DO (mg/L) S<< "*3- $L\ 1.1 £.P ^.0 750 rag KCI/L f&^-Xry. /-'*; ^fc;^ Conductivity (umhos/cm) /6>2o K-2S JC&O (5fO ^Temperature (°C) -fc\-\o T>\-3 I'M -iA-2>> -V\.\o -bi-C pH (S.U.) "3.a& ?*<<? ^9Z -7.5% 1.90 ^rbo DO (mg/L) Si =?., $.2. fr.a £3 an, 900 mg KCI/L Conductivity '*_-?l"?ft;r";; ig-MO {umhos/cm) /<?£%? , *l'*iC>> ' v'"' f -
mo
^Temperature (°C) "tel-io "Lii 1S-D "U<<b *^*s pH(S.U.) *7-ff6- ^7.% -=f.<93 7&Z T.VO ;Ui DO (mg/L) >>i ^7.ff 8.1 9.Z £3 ^^ 1050 mg KCI/L Conductivity ,*JSS/' _i',i ? , (umhos/cm) 27*? zr=k> HUO
Temperature (°C) "HU IH-H ^4.1 TA.T TM.U -i+t Initial Final Initial Final Initial Final l

Teniperaluresperformed at the time of test initiation, renewalor terminationby the analyst identified in the Daily Renewal Information table located on Page I.
Alkalinity and hardness performed by the analystidentified on the bench sheetspecific foreach analysis and transcribed to tliis benchsheetby: *\A SOP AT2 i - Exliibit AT21. i, revision 11-01-14 Page 76 of 91 f pS3ET" Page 6 of6 ^i environmentalTiefllngSolutiam,lnc Species: Pimephales promelas PpKCICR Test Number: 44 Day (Analyst identified for each day, pcrfonncd pH, D.O. and conductivity measurements only.) 3 4 5 6 Analyst ^ <<S- ftS^ 75 T5 /vr rtP "7^ Concentration Parameter t\yK :*:: \
7 "* ^.""7- ;^* :
S-":\ -."" pH(S.U.) 4-uM -}.i? -*.So 7M\ 1.70 3..3Z. -*4^ 7.^15 DO (mg/L) ^^ 14 -?.* l.<* 7.7 ^ ?** 7.7 Conductivity ^:i>:#!'W; * *'*,-.;;,-.;".;"' (umhos/cm) 30D tffru* 3,3 3i5 CONTROL 'Alkalinity l^:Sf!:i'fe? 111113 \.v>>* ,C-';*, **'^;- ;;.! (mgCaC03/L) \*% .y.y-^:,-: ss V.v* ' r ^^'^4'>>':'*
Hardncss (mgCaCOi/L)

nfv go "X ,l rV C*"": !: .'

Temperature TA-V* M..^ -iH.n TA-i -uvn a4.W "bLv M-h CC) pH (S.U.) aa> 1.14 *3.1*1 7. H*-l 7.74 ?.s^ ^32 7.37 DO (mg/L) ^ ^ -f.s 7.S 7.% V.3 o-o 7?
300 mg KCI/L Conductivity &z4 ;;... 77 ;0; '**) S';ipff^ (umhos/cm) ws m g=ri &-'/:*~< 'l:'i~'\' * .'
Temperature ~L*A *Vl.b "Vi.V>> *vA-s -\>\.i -lA-i- tMA -*M-S C°C>
pH(S.U) *\.e>3> T-02. T.UM- 743 7.76 1.3Z. 771 737 DO (mg/L) w 7-1* ->>-->> 79 7? ^?-3 &i 7? 450 mg KCI/L Conductivity (umhos/cm) lo^o 'M^Sffl Ilio IIHO ""*'* 'i *;*%*']. mo
Tempcrature
-1>V1 TMb "bl-Vo -v>l-s *v\-t "U-V2_ *w.w 1A.-1. (°C) pH (S.U.) "V-fl?. "*.l+ T-.v^q. 7*f6 7.77 T.*sr ^-fe'?' 73* DO (mg/L) a3 T-.T- IX 7.? 7? ?.2 ff.l ?.o Conductivity §1113 r ^"-: ** * *.! 600 mg KCI/L (umhos/cm) fcio Bio M3Q mo
Temperature *\it-b \q.4 1H.S CO lA-1 -\H.u iH.-\ ~b\.W -u\.i-pH(S.U) ^J&z ^.2\ T-.
>*?* 3,S 7? 79 *^2 ff.v 9.0 750 mg KCI/L Conductivity fotmhos/cm) I6<<t> V 1 1. V'- * *>> l<e3o 'v^'.-"'.:^-'1,- \ :' 17/0
/6)VO '"7:j;lM;l,:.:;;. ^Temperature X4 -tel-1 lA."T_ 1-1. U -ui.n *b\.n "bUU. mM (°C) pH (SM.) ^4 ~*.\<\ *?--?\ 7.Co\ 7.e/ "7-V/? ^.<<^9 739 DO (mg/L) QO 7-n- -** £.0 S?.<3 ^.2 ff-v <<./ 900 mg KCI/L Conductivity (umhos/cm) i&Sb Rro
. ""r

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^Temperature 1AZ -vv.-s *WJo 1H-S t.4-> ^?H&9 7 fO DO (mg/L) QA T-.<^ ^s 9.0 K.O 1.o {?.<<. ?.X> 1050 mg KCI/L Conductivity (umhos/cm) *alto M§ho i'.'-V-VJr-'-C'!: 2)2-0 2210 ^""'"*ii^&l'H ?/7<<? ^Temperature T.4-1 t^i -L*\.T- TH.U. TM-T- iH.i -lH.S PC) Hu/ Initial Final Initial Final Initial Final Initial Final eraturesperformed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Infonnation tablelocated on Page I. itynnd hardness pcrfonncd bythe analyst identified on the bench sheet specific for each analysis and transcribed tothis bench sheet by: X\ SOP AT21 - Exhibit AT21.1, revision 11 14 Page 77 of 91 V. >>*. << :& !'j.t;vtfjjgviV. Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Environmental Testing Solutions, inc. Source: In-house Culture 1.12 1 i I I i I I i 1 1 1 I 1 1 1 1 1 ' 1 T" 1 1.10 Control Limits (+/- 2 Standard Deviations) 1.08 h <<. <<- *"**-- ~-~ "*" *"~" """" *"* e © ©
© 0 o © -
_ £ © ^ 1.06 w <<3*aa>> ^.^ ^ ^ Q ^_ ^ ^, _ _, _ _ _ u 1.04 * - 03 z -J 1.02 m CM iiir iir ir i 1 1r u > 1.15 TO Warning Limits rs 1.10 1.05 1.00 0.95 1' ^>>t7>>>>>>>>>>>>>>>>>* Test date 7-day IC^ = 25%inhibition concentration. An estimation of the sodium chloride concentration which would cause a 25% reduction in Ceriodaphnia reproduction (calculated using ToxCalc). Central Tendency (mean logarithmic IC,5 converted to anti-logarithmic values) Control Limits (mean logarithmic IC25 +/- 2 standard deviations converted to anti-logarithmic values) Laboratory Warning Limits (mean logarithmic 1C25 +/-2 coefficent of variations converted to antf-logarithmic values) USEPA Warning Limits (mean logarithmic IC25 +/-S^10 converted to anti-logarithmic values, SA10 =10th percentile ofCVs reported nationally by USEPA) 1 Reviewed 6? Page 78 of 91 # ^vmp iwy- Ceriodaphnia dubia Chronic Reference Toxicant Control Chart ^l Environmental TimlrtflSotiHtom. Inc. s ource: iin-house C ulture o Logi0 Conversion Anti-logarithmic Values (g/L NaCl) Test number Test date 7-day IC2S Laboratory Calculated CV 10th Percentile CV ToxCal Determination 7-day IC^ CT s cr Control Limits Warning Limits Warning Limits (g/LNaCi) CT-2S CT+2S CT-2CV CT + 2CV CT-SA,10 CT + S^ 1 05-02-17 1.0691 0.0290 0.0275 0.0028 1.0654 1.0516 1.0794 1.0525 1.0783 0.9802 1.1506 2 06-06-17 1.0614 0.0259 0.0276 0.0027 1.0657 1.0524 1.0791 1.0532 1.0781 0.9804 1.1509 3 06-06-17 1.0518 0.0219 0.0275 0.0029 1.0653 1.0511 1.0798 1.0520 1.0787 0.9801 1.1506 4 07-11-17 1.0631 0.0266 0.0275 0.0029 1.0654 1.0512 1.0798 1.0520 1.0787 0,9602 1.1506 5 08-08-17 1.0810 0.0338 0.0277 0.0032 1.0660 1.0502 1.0820 1.0512 1.0807 0.9807 1.1513 6 09-12-17 1.0527 0.0223 0.0274 0.0034 1.0650 1.0485 1.0818 1.0495 1.0805 0.9798 1.1502 7 10-03-17 1.0540 0.0228 0.0272 0.0035 1.0646 1.0474 1.0821 1.0484 1.0807 0,9794 1.1497 8 11-07-17 1.0528 0.0223 0.0270 0,0037 1.0642 1.0463 1.0824 1.0473 1.0810 0.9790 1.1493 9 12-05-17 1.0469 0.0199 0.0269 0.0039 1,0638 1.0449 1.0832 1.0460 1.0817 0.9787 1.1489 10 01-09-18 1.0826 0.0345 0.0274 0.0042 1.0651 1.0446 1.0859 1.0458 1.0843 0.9799 1.1503 11 02-06-18 1.0500 0.0212 0.0270 0.0044 1.0642 1.0427 1.0862 1.0440 1.0844 0.9791 1.1494 12 03-06-18 1.0697 0.0293 0.0272 0.0045 1.0646 1.0430 1.0867 1.0443 1.0849 0.9794 1.1498 13 04-03-18 1.0835 0.0348 0.0273 0.0047 1.0649 1.0424 1.0880 1.0437 1.0861 0,9797 1.1501 14 04-03-18 1.0827 0.0345 0.0274 0.0048 1.0651 1.0420 1.0888 1.0434 1.0869 0.9799 1.1503 15 05-08-18 1.0822 0,0343 0.0275 0.0049 1.0655 1.0415 1.0899 1.0430 1.0879 0.9802 1.1507 16 06-05-18 1.0696 0.0292 0.0275 0.0049 1.0653 1.0415 1.0897 1.0430 1.0877 0.9801 L1506 17 07-10-18 1,0694 0.0291 0.0276 0.0049 1.0656 1.0417 1.0900 1.0432 1.0880 0.9804 1.1508 18 08-07-18 1.0555 0.0235 0.0274 0.0050 1.0652 1.0409 1,0900 1,0424 1.0879 0.9800 1.1504 19 09-11-18 1.0682 0.0287 0.0276 0.0050 1.0655 1.0413 1.0903 1.0428 1.0883 0.9803 1.1508 20 10-09-18 1.0640 0,0269 0.0275 0.0050 1.0654 1.0412 1.0902 1.0427 1.0882 0.9802 1.1507 Note: 7-day IC25 = 25%inhibition concentration. Anestimation of the sodium chloride concentration that would cause a 25% reduction in Ceriodaphnia reproduction (calculated using ToxCalc). CT= Central tendency of the ICW values. 5 - Standard deviation of the IC25 values. Control Limits = Mean logarithmic 1C25 +/- 2 standard deviations converted to anti-logarithmic values. Warning Limits = Mean logarithmic ICj*+/- 2CVor SA ,G converted to anti-logarithmic values. sa.io - Siandatd deviation corresponding to the 10* percentile ofCVs reported nationally byUSEPA (SM0 =0.08). Micro? **>>} CV = Coefficient of variation. I <<wi<<WBfnut TMUQ9SontUcn%,tru. Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture 40 1 1 1 1 I I I l ! ! 1 1 i 1 1 1 l 1 1 i - £ o -35 CD 0 <<~-~<<>> <<..u<<w ^...*-,<<>> <<<<<<<<. tt 03 o © o © 3 e 30 b7 & © 1 "O Cl) o H- &* ^^ a ^^ 0J bo 25 or c Jb-o a
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+*>> c o USEPA Minimum Acceptance Criteria (> 15 offspring per surviving female) o u 15 2 10 I << 111)1 1 1 1 1 1 1 I 1 1 1 1 1 1 l ! I i 1 I 1 i 1 1 1 1 1 1 1 I j 1 l l 40 USEPA Maximum Coefficient of Variation Guidance Criteria (< 42%), NCDEQ Maximum (< 40%) - 30 P 20 t5 f - +- o C +/-; o o 10 <<<<.<>>>. .*<<, *->>, <<=>> <<* <<>> <<>> ^jg>> -*>>*>>* ***0">> <<* **>> <<*>> <<~ <<<<* *a>> <<*^ *>>>> <<"*>> v.*, ****>> >><<*<<<<>> xra. ^ ^ ^^ <<_, .^ .*> ~ © o ,2 0 - l 1 1 - 50 . 1 1 1 I 1 1 1 1 1 I 1 1 1 1 1 1 1 1 1 1 . USEPA Upper PMSD Bound Acceptance Criteria (< 47%) - USEPA 30 - Lower PMSD Bound - Q in (>13%) / a. 20
j 10 -
I 1 1 1 I 1 1 1 1 1 1 I 1 I 1 1 1 1 1 1
Test date Control Reproduction, Coefficient of Variation (CV) or Percent Minimum Significant Difference (PMSD)
PMSDis the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is not a minimum acceptance criteria. rnt<<fed end Central Tendency (mean Control Reproduction, CV or PMSD) BevtcwMby if m Sumner 95% Confidence Interval (mean Control Reproduction, CV or PMSD +/- 2 Standard Deviations) Page 80 of 91 Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria I>r.h<<.i>>>>n)<< WiHOJ WWtwi, tor. Source: In-house Culture o ToxCal Determination Control Reproduction Control Reproduction CV Test PMSD -A Control Control Reproduction Test (offspring/female) m 1%) Test number Test date Survival Mean CV MSD PMSD CT 95% Confidence Interval CT 95% Confidence Interval CT 95% Confidence Interval (%) (offspring/female) (%) (%) CT - 2S CT + 2S CT-25 CT + 2S CT-2S CT + 2S 1 05-02-17 100 31.0 5.9 1.894 6.1 30.2 27.4 32.9 5.1 2.3 8.0 5.8 4.3 7.3 2 06-06-17 100 30.7 4.9 1.968 6.4 30.2 27.4 32.9 S.2 2.5 7.9 5,9 4.6 7.3 3 06-06-17 100 33,1 4.6 2.050 6.2 30.2 27.3 33.2 5.1 2.5 7.7 5.9 4.6 7.3 4 07-11-17 100 31.0 4.8 1.661 5.4 30.2 27.3 33.1 5,0 2.5 7,6 5.9 4.6 7.3 5 08-08-17 100 28.7 4.7 1.668 5.8 30.1 27.2 33.1 5.0 2.4 7 5 5.9 4.6 7.3 6 09-12-17 100 30.3 6.8 2.191 7.2 30.2 27,3 33.1 5.0 2.4 7.7 6.0 4.5 7.4 7 10-03-17 100 32.2 3.5 1.926 6.0 30.2 27.3 33.1 4.9 2.2 7,7 6.0 4.6 7.4 8 11-07-17 100 32.3 7.4 2.21S 6.9 30.2 27.3 33.2 5.1 2.3 8.0 6.1 4.7 7.5 9 12-05-17 100 28.6 6.0 2.344 8.2 30.3 27,4 33.1 5.2 2.5 8.0 6.2 4.5 7.9 10 01-09-18 100 29.7 4.5 2.015 6.8 30.1 27.4 32.8 5.2 2.5 8.0 6,3 4.7 7.9 11 02-06-18 100 29.3 5.6 1.824 6*2 30.1 27.4 32.8 5.2 2.5 7 9 6.2 4.7 7.8 12 03-06-18 100 30.6 6.8 1.995 6.5 30.1 27.4 32.8 5.2 2.4 8.0 6.2 4.7 7.8 13 04-03-18 100 28.0 4.5 1.729 6.2 30.1 27.3 32.9 5.3 2.6 7.9 6.2 4.7 7.8 14 04-03-18 100 31.8 5.5 2.108 6.6 30.2 27,4 33.1 5.3 2,7 7.9 6.3 4.7 7.8 IS 05-08-18 100 30.3 5.4 2.172 7.2 30.3 27.5 33.1 53 2.7 8,0 6.4 4.9 7.8 16 06-05-18 100 31.5 5.6 2.469 7.8 30.3 27.5 33.2 5.5 3.0 79 6.5 5.0 8.0 17 07-10-18 100 28.8 4.9 1.598 5.5 30.4 27.7 33.1 5.3 3.3 7.2 6.4 4.9 8.0 18 08-07-18 100 31,3 3.0 1.806 5.8 30.5 27.7 33.2 5.2 3,1 7.4 6,5 5.0 7.9 19 09-11-18 100 29.9 5.8 1.943 6.5 30.5 27.7 33.2 5.3 3.1 7,4 6,5 5.0 7.9 20 10-09-18 100 29.5 8.2 1.912 6.5 304 27.7 33.2 5.4 2.9 7.9 6.5 5.1 7.9 Note: Control Survival - USEPA minimum test acceptability criteria > 80% survival. ControlMean Reproduction<< USEPA minimum test acceptability criteriaa is offspring/surviving female CV= Coefficient of variation for control reproduction USEPA maximum CV guidance criteria (90,h percentile) <42%. NCDfTQ maximum CV acceptance criteria <40%. MSD = Minimum significant difference. PMSD = Percent minimum significant difference. PMSD is a measure of test prebsion, ThePMSD isthe minimum percent differencebetween the controland treatment that can be declaredstatistically significant Ina wholeeffluenttoxicity test. Lower PMSD bound determined by USfcPA {10,h percentile) >13%. The lower PMSD bound represents a practical limitto the sensitivityof the test method and is not a minimumacceptance criteria Upper PMSD bound acceptance criteria determined byUSEPA (9Q,H percentile} c 47%. CT << Central tendency of the reproduction, CVor PMSD values. S - Standard deviation of the reproduction. CV or PMSD values. tf Page I of6 _>> la<<lronmewUJTrtth>95efta>>><<H<<c Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphma dubia CdNaCICR#: 220 Dilution preparation information: Comments: NaCl Stock INSS number inss r\c& Stock preparation: lOOgNaCi/L: Dissolve 50 g NaCl in 500 mL Milli-Q water. Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 IS 21 Diluent volume (mL) 1491 I4S8 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test organism source information: Test information: Organism age: < 24-hours old Randomizing template color: ftvAWL Date and times organisms were bom io-O^IS' c*>>vS "TO fitoS Incubator number and shelf between: Culture board: IO-CVl£ tK location: 19>\ Replicate number: i 2 5 j 4 5 6 7 s 9 to YWT batch: Culture board cup number. 1 JO n Ivx n K "U>> xs ao 31 U-OHf Transfer vessel information'. pH=n.£S S.U. Temperature = -\&*\CC Setenasdum batch: Average transfer volume (mL): < 0.25 mL J.0>>ffU\f Daily renewal information: Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0 A 10-09-18 olMl ivca-iS 1 J 10-10-18 oJSb la-rji-ti 2 10-11-18 oH& la-crv* i £ i If 0 10-12-18 lO-ff\-V& 0*41 4 \\ u A 10-13-18 a^S ro-iH* fc 5 10-14-18 6 0££t> io-iY-\4 rs ft 10-15-18 o&4Sr IO -\\-\ & (\ -ii 7 10-16-18 oHf '.v-'l-.IU.iVfiia7. '*'Z.'j Control information: Acceptance criteria Summary oftest endpoints: % of Male Adults: 01. 520% 7-day LCso >l4cr\ % Adults having 3rd Broods: 1007. > 80% NOEC tocn % Mortality: 07. <20% LOEC tioo Mean Offspring/Female: 7ft J^ > 15.0offspring/female ChV Itftt.S % CV: <40.0% Ids Mr* IPVf- ° SOP ATM - Exhibit ATI4.1, revision I t-OI-14 Page 82 of 91 O If 'J. ill it<Y; Page 2 of6 , trwtnflrMnulTmtiagScttMMtwtm. Species: Ceriodaphnia dubia CdNaCICR#: 220 CONTROL Survival and Reproduction Data Replicate number Day 1 10 Young produced c -O- ja H. -CL Adult mortality u \_ \- v_ v_ V- V Young produced XL Adult mortality ^ V_ V_- Young produced o O XL o JS. -Q_ Adult mortality Young produced M Adult mortality Young produced ID IO IO >0 \V *o lT_ _fL Adult mortality v_ Young produced o J^L Adult mortality u Young produced I "I i3 14 \K* lA. t\o U 1+/- Total young produced
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^^ T>2-Final Adult Mortality V TT" X for 3rd Broods X yg. s FTT^ V- JS*. - ^ H. A'ott- Adultmortality (L= live, D=dead), SB = splitbrood (sinsle brood splitbetween twodays), CO= cany over(offspring carried over with adult during transfer). Concentration: % Mortality: Ol. Mean Offspring/Female: ~U\& 600 mg NaCl/L Survival and Reproduction Data Replicate number Day 10 Young produced jCl _a o O Adult mortality U u V^ Young produced a O a _A Adult mortality L_ L_ L. Young produced o O Adult mortality l_ v_ U V_ U Young produced Adult mortality V. t> Young produced 10 >>1s to >l '0 IL. iO Adult mortality Young produced o £l XL XL _Cl _£l XL Adult mortality v_ Young produced \\o tu >>"b lU \u>> VS IS. V2>> 15 Total young produced *2H 1f\ "2-<\ "2A bo 32. 15 1.9 ii 5o Pinal Adult Mortality _L_ Note: Adultmortality (L = live, D= dead), SB =splitbrood fsingle brood splitbetween twodays), CO=carry over(offspring carried over with adult during transfer). Concentration: % Mortality: Ot Mean Offspring/Female: -2<<V<=> % Reduction from Control: - 0.-& 7 SOP AT14-Exhibit AT14.1, revision 11-0M4 Page 83 of 91 iet1 Page 3 of6 J lavtoa&a>eftUttratika.tofixie<tfctac Species: Ceriodaphnia dubia CdNaCICRJ: 220 800 mg NaCI/L Survival and Reproduction Data Replicate number Day 1 10 Young produced O o Jd XI _Q_ o Adult mortality l_ Young produced o _D_ XL D XL Adult mortality v_ \_ Young produced XI _D_ 0 _D_ _o XL o Adult mortality v_ Young produced q Adult mortality Young produced u v\ ro IO IO IV tfc Adult mortality v Young produced o XL XL Adult mortality Young produced i4 >>T_ i4 *S vS \Vo i4 ll i^. l"L Total young produced x°\ -L°\ *50 "U "2>0 Tsl ^st> TJio IS -tf Final Adult Mortality Note: Adultmortality (L~ live, D- dead), SB - split brood (single brood splitbetween twodays), CO>> carry over (offspring carried over with adult during transfer). Concentration: % Mortality: 07. Mean OfTspring/Female: Tft.V % Reduction from Control: \.4 7. 1000 rag NaO/L Survival and Reproduction Data Replicate number Day 10 Young produced O o o o Adult mortality -£ XX V. L_ Young produced O XI XL XL JX XL Adult mortality L_ ^ Young produced Adult mortality ^ n H. XL v__ XL U *t o o XI Young produced Adult mortality v^ v_ Young produced JQ to il IO l-L II IO Adult mortality Young produced O XL JX Adult mortality V^ \^ Young produced ^ i4 >>' IS IS 1^ IX 13* ii Total young produced 1*\ ir\ i<> ho 1% IS 1°k ^O Final Adult Mortality c Note: Adult mortality (L = live, D - dead), SB = split brood (single brood split between two days),CO* carry over(offspring carried over with adult during transfer). Concentration: % Mortality: 07. Mean Offspring/Female: XI .7 % Reduction from Control: U7. SOP AT 14 - Exhibit AT 14.1, revision 11-01-14 Page 84 of 91
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? £miwmm*cUtTe>>PngW*j>>to<<*f<t£. Species: Ceriodaphma dubia CdNaCICRfc 220 1200mgNaCI/L Survival and Reproduction Data Replicate number Day 10 Young produced o _Q_ Xl XL o XI Adult mortality \_ Young produced O O O XL Adult mortality Young produced o O O XL XX n JQ_ J2l Adult mortality V_ Young produced *A +/-L +/-L ^T Adult mortality Young produced G o o _ 5 Young produced o -L- O o O 0 0 \ 0 O Adult mortalUy u \ v_ V v_ v_ v_ v_ u. \_ 6 Yonng produced o 0 0 0 0 O a 6 6 0 Adult mortality u V v_ v_ \_ V_- ^ v_ v_ v_ Young produced 0 0 0 6 0 0 0 7 0 o 0 Total you tig produced X. \ "L 7_ I i ^ I \ T_ \_ \_ \ v_ ^_ v^ Final Adult Mortality V V- \ v Note: Adult mortality (L = live, D = dead), SB<< splitbrood (single brood splitbetween twodays), CO~ carry over(offspring earned over with adult during transfer). Concentration: % Mortality: m Mean Offspring/Female: l.W % Reduction from Control: 4M.fc7. SOP ATI4- Exhibit ATI4.1, revision li-Ol-14 Page 85 of 91 ^tOQS^Bed 00 o o 2 o o o o o
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O O at o <<< <<< O 3 3 a. 3 c cm oq OX) 2 ft M o o o I-* o o o M -O o o u> o O o H>> A o flj in O o o o H>> io 5 OJ o' n n 3 M o H o o ro o o o ro oo o o *>> A o a o to r->> O to £t o o o ro oi UJ O i-t-OJ ro o o o NJ o a o UJ to o un o A o UI ro in o o O s c o o UJ >^ o o o h* o o o *>> o o UI o o o *>> O W o o o A UJ o K K s 39 T7 ro m ?? "SL "A o © o *-* o o o Vf n ro fO o n* UJ tn o o o VI o o o o v>> OJ £ o 01 o s r>> ro rt ro 3 3 3 UJ o o o UJ o a en C ch A Cl C ro C o o o o o o o U> o o o en O VI ro s 3 3 3 or a* ro ID ro l->> o o o M o o o ^1 to o o un J3k o o o VJ o UJ o o o *sf a G ro I-* o a M o o o o oo vl o o UI Jbk o o o 00 a to H o o o 00 u in ro o o o IO o o o to o O in o o o 10 o Ui o o a to VI s u v>> ro o a o ro o o o ID o ->j UJ in o o M a o o UJ o o o o s o ro s o1 UJ UJ UJ o1 o o u o o o o o o g y o o o o o £S ^4 oa in to g ff M 4^ mi E ro oo Ceriodaphnia dubia Chronic Reference Toxicant Test o EPA-821-R-02-013, Method 1002.0 to Envlronment.ilTesting Solutluns. Inc. Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number? CdNaCICR<<220 Test dates: October 09-16,2018 Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction (mE/LNaC!) 1 2 3 4 5 5 7 a 10 (offspring/female) variation [%) from control (%) 9 Control 29 33 33 30 22 32 28 28 ?8 7g 1.00 29.5 8.2 Not applicable 600 29 29 31 30 29 32 29 28 29 30 100 29.6 4,0 -0.3 800 29 29 30 28 30 35 30 26 28 29 100 29.1 5.5 1.1 1000 29 2b 27 23 30 25 28 28 .". 30 100 27.7 6.8 6.1 1200 10 8 9 23 H 10 g 7 15 9 100 10.a 25.7 61.7 1--100 2 2 1 *j 7 j 3 i r 100 1.6 13,7 94.6 Dunnett's MSD value: .912 MSD = Minimum Significant Difference PMSD: PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant In a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10 percentile) = 13%. UpperPMSD bounddetermined byUSEPA (90"' percentile) =47%. Lower and upper PMS0 bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA'sWET interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b), The lower PMSDbound represents a practical limit to the sensitivity of the test method and Is not a minimum acceptance criteria. USEPA. 2001a, 2001b Final Rapor t; Inteflaboratory VariabilityStudy of EPAShort-term Chronic and Acute Whole Effluent ToxicityTest Methods, Volumes 1 and 2-AoDendix. EPA-S21-B-01-004 and EPA-821-B-Q1-005. us Environments! Protect ion Agency Cincinnati. OH 2 Statistical Analyses Envf ronrrxmtal letflng SoluMenv fnc Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 10/9/2018 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Dale: 10/16/20 ie Lab ID: ETS-Envir. Tasting Sol Sample Type: NACL-Sodium chloride Sample Dale: Protocol FWCMR-EPA-821-R-02-Q13 Test Species: CD-Cenodaphnia dubia Comments: Cortc-mg/L 1 2 3 4 5 6 7 8 9 10 D-Control 29-000 33.000 33.000 30.000 26.000 32.000 28.000 28.000 28.000 28,000 600 29.000 29.000 31,000 30.000 29 000 32000 29.000 28.000 29.000 30.000 800 29.000 29 000 30.000 28.000 30,000 32,000 30.000 26,000 28.000 28,000 1000 29.000 26.000 27.000 25.000 30.000 25,000 28.000 28,000 29.000 30.000 1200 10.000 8.QG0 9.000 13.000 14.000 10.000 9.000 7.000 15000 9.000 1400 1.000 2.000 2.000 1.000 1 000 3.000 1.000 1.QQ0 2.000 2.000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 29,500 10000 29.500 26.000 33.000 8.187 10 29.550 1.0000 600 29.600 10034 29.600 28.000 32.000 3.965 10 113.00 75.00 29.550 1.0000 800 29.100 0,9864 29.100 26.000 32.000 5.482 10 105.00 75.00 29.100 0.9848 1000 27 700 Q.9390 27.700 25.000 30.000 6.818 10 86.50 75.00 27.700 0.9374
1200 10.400 0.3525 10.400 7.000 15.000 25,721 10 55.00 75.00 10.400 0,3519

1400 1.600 0.0542 1.600 1.000 3.000 43.700 10 55.00 75.00 1.600 0.0541 Auxiliary Tests Statistic Critical Skew Kurt Koimogorov D Test indicates normal distribution (p > 0.01) 0.87486 1.035 041935 0.0382 Bariiett's Test indicates unequal variances (p = 4.59E-03) 16.954 15.0863 Hypothesis Test (1-tatt, 0.05) NOEC LOEC ChV TU Steel's Many-One RanK Test 1000 1200 1095.45 Treatments vs D-Controi Linear Interpolation (200 Resamples)
Point mg/L SD 95% CL Skew IC05 946.786 102.278 673J23S 10D8.67 -1.5854 IC10 1012.77 21 5456 930.85 1025.48 -2 8515 IC15 1029.86 8.05625 1012.26 1042-05 -0.2255 IC20 1046.94 7.60606 1030.73 1058.6 -0.2148 IC25 1064,02 7 31883 1048 23 1075.01 -0.2050 IC40 1115.26 7.56893 1098 97 1129.87 0,0321 IC50 1149.42 857853 1131.79 1167.31 0 3597 1000 Dose mg/L Dose-Response Plot ^ Page 88 of 91 Statistical Analyses Environmental Testing Solutions, Inc. Used for PMSD calculation only. Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 10/9/2018 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 10/16/2018 Lab ID: ETS-Envlr. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: RA/CHR-EPA-821^R-02-013 Test Species: CD~Ceriodaphnia dubia Comments: Conc-mg/L 8 10 D-Control 29.000 33.000 33.000 30.000 26.000 32.000 28.000 28.000 28.000 28.000 600 29.000 29.000 31 000 30.000 29.000 32.000 29.000 28.000 29.000 30.000 800 29.000 29.000 30 000 28.000 30.000 32.000 30.000 26.000 28.000 29.000 1000 29.000 26.000 27.000 25.000 30.000 25.000 28.000 28.000 29.000 30,000 1200 10.000 8.000 9.000 13.000 14.000 10.000 9.000 7.000 15.000 9.000 1400 1.000 2.000 2.000 1.000 1.000 3.000 1.000 1.000 2.000 2.000 Transform: Untransformed 1-Tailed Conc-mg/L Mean N-Mean Mean Min Max CV% t-Stat Critical MSD D-Control 29.500 1.0000 29.500 26.000 33.000 8.187 10 600 29.600 1.0034 29.600 28.000 32 000 3.965 10 -0.120 2.287 1.912 800 29.100 0.9864 29.100 26.000 32.000 5.482 10 0.478 2.287 1.912 1000 27.700 0.9390 27.700 25.000 30,000 6.818 10 2.153 2.287 1.912
1200 10.400 0.3525 10.400 7.000 15.000 25.721 10 22.847 2.287 1,912

1400 1.600 0.0542 1.600 1.000 3000 43.700 10 33.373 2.287 1.912 Auxiliary Tests Statistic Critical Skew Kurt Koimogorov D Test indicates normal distribution (p > 0.01) 0.87486 1.035 0.41935 0.0382 Bartiett's Test indicates unequal variances (p = 4.59E-03) 16.954 15.0863 Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 1000 1200 1095.45 1.91164 0.0648 1489.66 3.49444 4.4E-42 5.54 Treatments vs D-Control
^ Page 89 of 91 f7UET^j^3 Page 5 of6 . (<utfBMneiain<<B*gfott!U>AtIac Species: Ceriodaphnia dubia CdNaCICR #: 220 Daily ChemistryK Day (Analyst identified foreach day, performed pH, D.O. and conductivity measurementsonly.) 0 1 2 Analyst MS MS MX TS TS I/O Concentration Parameter SSisSf; WrlWa PfSPS! mum !?lliillSll pH (S.U.) ?&<>> ^.8*t "=V=fs 7.87 7.59 <U*^ DO (mg/L) *7-? l.<3 ^0 &o 7-9 aPl Conductivity (pmhos/cm) 311 3/t* 30A CONTROL *Alkalinity <dO V* <><\ -v (mgCaCOs/L)
Hardncss (mgCaC03/L) *4 :^7".V"^4'; So

Temperature (°C) i.<<U8 -6.o -*\A' X4-* *x\.\+ -\it*
pH (S.U.) ^8\ *3-ff-</ ^-s*? 7M 777 Ci^-v DO (mg/L) l.e "** ^fe %.o 7? A^ 600 mg NaCI/L Conductivity (umhos/cm) Wo tS2o *%^rif^ ~r. mo <<L'i#fe<<uJV^ ^Temperature (*C) -uM T.AA -^.8 "MI-Vd TA-i -w-<<\ pH (S.U.) 3-SZ itg"/ "^.fffi 7.81 7.?0 w DO (mg/L) 18 7.<? 1J.0 9.0 -7.9 ©*o 800mgNaCI/L Conductivity *>'* fJ*' ^ ~ >> >>. (jimhos/cm) ie&o /fl>>0 HAG ^Temperature (°C) j TH-f ^-^ I -l<L1 ^*1 x*VA pH(S.U) \^f>Z
\<~A y.Bi ~7.tf? 7.2S 723 ^(^
DO (mg/L) ^f ^<? ^<F &./ 79 0.O lOOOmgNaCl/L Conductivity (umhos/cm) Z/So 1Z3o f:m^> .2/6(3
Temporaturc (°C) *LH.g tsL* TAA *v>.o xA-n TS.D pH(S.U) ¥.ffZ Tr!*£T %8<i T23 7 J?.? ^U-0 DO (mg/L) ^-7 \<* ^<? S./ 7.9 ©.o l20OmgNaCl/L Conductivity (umhos/cm) 2&>oo \*&f$'t Z660 c^O

Temperature (°C) *V*,£ *vl-1 "\S-X) WA *fctfe -utg pH (S.U.) ^8H "=?.*& 7.?l 7.BH 7Z4 a^2_
DO (mg/L) &*& 8~o 7.<r S.l 7.9 54 1400 rag NaCl/L Conductivity (umhos/cm) *2990 ^'*$*'*^3 Z&lo ^Mii 3&-70 ^Temperature (°C) -rt-f M-4 "is-c. t\.s *yl.l nH.*\ I Initial l Final Initial Pinal Initial Final Temperatures performed at ihctimeof testinitiation, renewal ortermination by theanalyst identified in dieDaily Renewal Informauon table located on Page 1. Alkalinity and ha/dness performed bythe analyst identified onihc bench sheet specific for each analysis and transcribed to this benchsheet by: SOP ATI4 - Exliibit ATI4J, revision 11-0M4 Page 90 of 91 s^ Page 6 of6 . tn>>Jjo<<racnUfT<<tagSofefttoitvt>>c. Species: Ceriodaphnia dubia CdNaCICR #: 220
Tcmpcraturcs performed at the time of tesi initiation, renewal or termination bythe analyst identified inihc Daily Renewal Information table located on Page 1. Alkalinity and hardness performed bythe analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: \/\
SOP ATM - Exliibit ATI4.I, revision 11-01-I4 Page 91 of 91

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