Text

Discharge Monitoring Report for October 2018
	ML18318A194
Person / Time
Site:	Sequoyah
Issue date:	11/08/2018
From:	Anthony Williams; Tennessee Valley Authority
To:	; Document Control Desk, Office of Nuclear Reactor Regulation
References
	Download: ML18318A194 (99)
	v • d • e

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name _ _IY* ^ro^YA^r^C^E^R^^NT M6ress_ _P.a.BOX_2poo_

aNIEROFFICEOPS^NLSQN)

SODDY_-^AJSYiXN_3Z3§4 Facjijty__TVA-SEQUOYAH NUCLEAR PLANT Juration. _HAMILTOJi_CO]unjY ATTN:Millicent Garland NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR01)

F - FINAL DIFFUSER DISCHARGE EFFLUENT

- - NO DISCHARGE

[^J ***

NOTE: Read instructions before completing this form.

Form Approved.

OMB No. 2040-0004 From TN0026450 101 G

PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 18 10 01 18 10 31 PARAMETER X

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG.

CENTIGRADE 00010 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT 39.3 04 DEG.C.

0 31/31 RCORDR PERMIT REQUIREMENT Req. Mon.

DAILY MAX CONTI NUOUS CALCTD TEMPERATURE, WATER DEG.

CENTIGRADE 00010 Z

0 INSTREAM MONITORING SAMPLE MEASUREMENT 27.1 04 DEG.C.

0 31/31 MODELD PERMIT REQUIREMENT 30.5 DAILY MX CONTI NUOUS CALCTD TEMP. DIFF. BETWEEN SAMP. &

UPSTRM DEG.C 00016 1

S EFFLUENT GROSS SAMPLE MEASUREMENT 2.5 04 DEG.C.

0 31/31 CALCTD PERMIT REQUIREMENT 3.0 DAILY MX CONTI NUOUS CALCTD FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT 03 MGD 0

31/31 RCORDR PERMIT REQUIREMENT Req. Mon.

DAILY MAX CONTI NUOUS RCORDR FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1

0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 1806 03 MGD 03 MGD 0

31/31 CALCTD PERMIT REQUIREMENT Req. Mon.

MOAVG CONTI NUOUS CALCTD CHLORINE, TOTAL RESIDUAL 50060 1

0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 0.016 0.028 19 MG/L 0

20/31 GRAB PERMIT REQUIREMENT 0.1 MOAVG 0.1 DAILY MAX FIVE PER WEEK CALCTD TEMPERATURE - C, RATE OF CHANGE 82234 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT 0.4 62 DEG C/HR 0

31/31 CALCTD PERMIT REQUIREMENT 2.0 DAILY MX CONTI NUOUS CALCTD NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Anthony L. Williams Site Vice President TYPED OR PRINTED ICertifyunder penalty of law that this document and all attachments were prepared under my" direction or supervision in accordance witha system designed to assure that qualifiedpersonnel properly gather and evaluatethe information submitted. Basedon myinquiry ofthe pepgon or persons who manage the system, orthose persons directly responsible for gatherinjnhe information,the informationsubmitted is, to the best of my knowledge and belief, tiV and complete. Iam aware that there are significantpenalties for submittingfalse information, including the possibility of fine and imprisonment for knowing violations.

SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE 423 843-7001 AREA CODE NUMBER DATE 18 11 YEAR MO COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference all attachments here)

No closed mode operation. The following injectionsoccurred: Flogard MS6236 (max calc. was 0.029 mg/L, limit is 2.0 mg/L), Spectrus BD1500 (max calc. was 0.031 mg/L, limit is 2.0 mg/L), and Spectrus CT 1300 (max calc. was 0.033 mg/L, limit is 0.05 mg/L).

EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page 1 of 1 08 DAY

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name _ JEY*^EQ^^m^CL^I^^NT Mdress_ _P QJBOX2000_

aNIEROFFICEOPS^lNLSON)

SODDY.-^AlSYj:N_37384

£acility__TVA.-^EQyOYAH NUCLEAR PLANJ Location JH/^llJpJi.TOUiiJY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (DMR)

MAJOR (SUBR01)

F - FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT

- - NO DISCHARGE Q

NOTE: Read instructions before completinq this form.

Form Approved.

OMB No. 2040-0004 ATTN:Millicent Garland From TN0026450 101 T

PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 18 10 01 18 10 31 PARAMETER X

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT

>100.0 23 PERCENT 0

3/180 COMPOS PERMIT REQUIREMENT 42.8 MINIMUM SEMI ANNUAL COMPOS IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT

>100.0 23 PERCENT 0

3/180 COMPOS PERMIT REQUIREMENT 42.8 MIMINUM SEMI ANNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under m]

direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluatethe information submitted. Based on myinquiry ofthe pen^n or persons who manage the system, or those persons directly responsible for gathering tl information, the information submitted is, to the best of my knowledge and belief, true, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.

Anthony L. Williams Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachments here)

Toxicity was sampled October 8-12, 2018.

EPA Form 3320-1 (REV 3/99)

Previous editions may be used itevice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE 423 843-7001 AREA CODE NUMBER DATE 18 11 YEAR MO Page 1 of 1 08 DAY

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name __ _IY* ^M^W^MC^EARJ^NT Address_ ^OJJOX200g_

aNIERpFR^QPS^NLSQN)

SODDY_-_DAISYj:N_37384 Facmty

_JVA-SEQUOYAH NUCLEAR PLANJ

^c^on_JjMILTOJi_TOUJirY ATTN:Millicent Garland NATIONALPOLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (DMR)

From TN0026450 103 G

PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 18 10 01 18 10 31 MAJOR Form Approved.

(SUBR 01) 0MB No-2°40-0004 F - FINAL LOW VOL. WASTE TREATMENT POND EFFLUENT

- - NO DISCHARGE

\\^J ***

NOTE: Read instructions before completing this form.

PARAMETER X

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH 00400 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT 6.6 7.7 12 SU 0

6/31 GRAB PERMIT REQUIREMENT 6.0 MINIMUM 9.0 MAXIMUM ONCE/

WEEK GRAB SOLIDS, TOTAL SUSPENDED 00530 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT 5.8 5.9 19 MG/L 0

1/31 GRAB PERMIT REQUIREMENT 30.0 MOAVG 100.0 DAILY MX ONCE/

MONTH GRAB OIL AND GREASE 00556 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT

<5.0

<5.0 19 MG/L 0

1/31 GRAB PERMIT REQUIREMENT 15.0 MOAVG 20.0 DAILY MX ONCE/

MONTH GRAB FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT 1.396 1.547 03 MGD 0

5/31 INSTAN PERMIT REQUIREMENT Req. Mon.

MOAVG Req. Mon DAILY MX ONCE/

WEEK INSTAN SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

")

y NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertifyunder penalty of lawthat this document and all attachments were prepared under my' directionor supervision inaccordance witha system designed to assure that qualifiedpersonnel properly gatherand evaluatethe information submitted. Basedon myinquiry ofthe pers6nor persons who manage the system, or those persons directlyresponsible forgathering the-information, the informationsubmitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significantpenalties forsubmittingfalse information, includingthe possibilityof fine and imprisonmentfor knowingviolations.

Anthony L. Williams Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

EPA Form 3320-1 (REV 3/99)

Previous editions may be used "Site Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE 423 843-7001 AREA CODE NUMBER DATE 18 11 YEAR MO Page 1 of 1 08 DAY

PERMITTEE NAME/ADDRESS (IncludeFacilityName/Locationif Different)

TVA -SEQUOYAH NUCLEAR PLANT Name.

Address_ _P.O_BOX2000.

aNIEROFRCE OPS^SQN)

S0DDY.-_DA]SYJ1N_37384 Facility^ _TVA_-^EQUOYAI+/-NUCLEAR PLANJ j£C£tjon_JdMiyPiLraUJNlJY ATTN:Millicent Garland NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

From TN0026450 110 G

PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 18 10 01 18 10 31 (SUBR01)

F - FINAL RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE

[xx] ***

NOTE: Read instructions before completing this form Form Approved.

OMB No. 2040-0004 PARAMETER X

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG.

CENTIGRADE 00010 1

o EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 04 DEGC PERMIT REQUIREMENT REPORT DAILY MX CONTIN UOUS CALCTD TEMPERATURE, WATER DEG.

CENTIGRADE 00010 Z

0 INSTREAM MONITORING SAMPLE MEASUREMENT 04 DEGC PERMIT REQUIREMENT 30.5 DAILY MX CONTIN UOUS CALCTD TEMP. DIFF. BETWEEN SAMP. &

UPSTRM DEG.C 00016 1

0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 04 DEGC PERMIT REQUIREMENT 5

DAILY MX CONTIN UOUS CALCTD FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1

0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 03 MGD PERMIT REQUIREMENT Req. Mon.

DAILY MX CONTIN UOUS RCORDR CHLORINE, TOTAL RESIDUAL 50060 1

0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 19 MG/L PERMIT REQUIREMENT 0.1 MOAVG 0.1 DAILY MX Five per Week CALCTD TEMPERATURE - C, RATE OF CHANGE 82234 1

0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 04 DEGC PERMIT REQUIREMENT 2

DAILY MX CONTIN UOUS CALCTD SAMPLE MEASUREMENT

^*~

PERMIT REQUIREMENT

)

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty oflaw thatthisdocument andallattachments were prepareduhder my direction or supervision inaccordancewith a systemdesignedto assure thatqualified personnel properly gather and evaluate the information submitted. Based on my inquiry o/fhe person or persons who manage thesystem, orthosepersons directly responsible for gathering the information, theinformation submitted is, tothebestofmy knowledge andbelieJUojer^^GfaTe, and complete. Iamawarethatthereare significant penaltiesforsubmitting falseinformation, includingthe possibilityoffine and imprisonmentfor knowingviolations.

Anthony L. Williams Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3/99)

Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE 423 843-7001 AREA CODE NUMBER DATE 18 11 YEAR MO Page 1 of 1 08 DAY

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA ^EQ^^Hj^CLEjAR^l^rs^

Address_ _P.a.BOX_2000.

aNIERQFFICEOPS^NLSQN)

S0DDY_-_DA]SYJ1N_37384 Facjiity_ JVA.-JEQUOYAH NUCLEAR PLANJ J^catLon_ Jj/WI!JpJl(^UMT ATTN:Millicent Garland NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (DMR)

From TN0026450 110 T

PERMIT NUMBER DISCHARGE NUMBER YEAR MONITORING PERIOD MO DAY To YEAR MO DAY 18 10 01 18 10 31 MAJOR (SUBR01)

F - FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE XX Form Approved.

OMB No. 2040-0004 NOTE: Read instructions before completing this form.

PARAMETER X

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1

0 0

EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 23 PERCENT PERMIT REQUIREMENT 42.8 MINIMUM SEMI ANNUAL COMPOS IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 10 0

EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 23 PERCENT PERMIT REQUIREMENT 42.8 MINIMUM SEMI ANNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT

^~~

PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify underpenalty of lawthatthisdocumentandallattachmentswere prepared*>>nder my direction orsupervision inaccordance with a systemdesigned toassure that qualified personnel Anthony L. Williams Site Vice President TYPED OR PRINTED properly gather andevaluate the information submitted. Basedonmyinquiry o/1heperson or personswhomanagethe system,orthose personsdirectly responsible for adheringthe information, theinformation submitted is, tothe best ofmy knowledge andZelief, true^^aecOrate, and complete. Iamawarethatthereare significant penaltiesforsubmittingTateeHflTormation, including the possibility of fineand imprisonment forknowingviolations.

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3/99)

Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE 423 843-7001 AREA CODE NUMBER DATE 18 11 YEAR MO Page 1 of 1 08 DAY

PERMITTEE NAME/ADDRESS (Include Facility Name/Location ifDifferent)

Name ___'A ^EQ^^^N^CLE^RJPLANT Address_ _P. a_BOX2000_

(irrTEROFFICEOPS^rlSQN)

SODDY.-_DA]SYJ1N_J7384 Faci!jty___ _TVA-.SEQUOYAH NUCLEAR PLANJ J^c^oiL.Jd^liJPJLCOUJirY ATTN:Millicent Garland NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (DMR)

From TN0026450 118 G

PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 18 10 01 18 10 31 MAJOR (SUBR01)

F - FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE XX Form Approved.

OMB No. 2040-0004 NOTE: Read instructions before completing this form.

PARAMETER X

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO) 00300 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT 19 MG/L PERMIT REQUIREMENT 2

MINIMUM TWICE/

WEEK GRAB SOLIDS, TOTAL SUSPENDED 00530 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT 19 MG/L PERMIT REQUIREMENT 100 DAILY MX TWICE/

WEEK GRAB SOLIDS, SETTLEABLE 00545 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT 25 ML/L PERMIT REQUIREMENT 1

DAILY MX ONCE/

MONTH GRAB FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT 03 MGD PERMIT REQUIREMENT Req. Mon.

MOAVG Req. Mon.

DAILY MX ONCE/

BATCH ESTIMA SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

^

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Anthony L. Williams Site Vice President TYPED OR PRINTED Certify underpenaltyoflawthatthisdocument andallattachmentswerepreparejklndermy direction orsupervision inaccordance with a system designed toassurethat (justified personnel properly gatherandevaluatetheinformation submitted. Basedon myinquipy^of thepersonor personswho manage the system, orthosepersonsdirectly responsible^or gathering the information, the information submitted is, tothebestofmyknowlecWand belief, true,accurate, and complete. Iamaware thatthere are significant penaltiesforsubmitting falsejj includingthe possibilityoffine and imprisonment forknowing violations.

SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period EPA Form 3320-1 (REV 3/99)

Previous editions may be used TELEPHONE AREA CODE 843-7001 NUMBER DATE 18 11 YEAR MO Page 1 of 1 08 DAY

ENVIRONMENTAL RECORDS PROCESSING FORM Title of File:

SQNJDctober 2018 Semi-Annual Toxicity Report for DSN101 Site/Plant/Project Name:

Sequoyah Nuclear Plant Accession Number (optional):

Work Order Number (optional):

Your Name:

Donald W. Snodgrass Date Submitted (YYYYMMDD):

20181109 Document Date (YYYYMMDD):

20181109 Instructions:

1)

Complete the Environmental Records Processing Form.

You must include a document date on the form.

Examples:

o Annual Report = Dec 31 of year report covers (20XX1231) o Manifests - shipment date o

Permit & Letters - submittal date Verify that only one box is checked for the Document Type.

2)

Save the entire document as a single PDF file with the Environmental Records Processing Form as the first page.

Name the file using the following convention: Site/Plant/Project Name_Title of file.pdf.

Notes:

o Site/Plant/Project Name - see EDMS Acronyms & Facility Abbreviations on Environment InsideNet Page for approved abbreviations Examples:

o CSC_ 2013 Annual Hazardous Waste Report.pdf o

WLH_February2014DMR.pdf.

3)

Email the PDF to ENVrecords@tva.gov for processing For assistance, please contact the Responsible Environmental Person for your site/project, the Environmental Media Specialists (See Contacts on Environment InsideNet Page), or your Business Support Representative.

AIR Air Compliance Records Air Correspondence Air Quality Permits & Applications Open Burning Permits & Records Refrigeration Technicians Certifications - Contractors Refrigerant Usage Logs & Surveillance Records SF6 Emission Reports ASBESTOS Asbestos Correspondence Asbestos Disposal Compliance Records Asbestos Medical Surveillance Records - Contractors D

Asbestos Sampling Compliance Records Asbestos Worker Certifications - Contractors DRINKING WATER Drinking Water Correspondence Drinking Water Laboratory Results Operator Certifications - Contractors ENDANGERED SPECIES Endangered Species Compliance Records Endangered Species Correspondence GROUNDWATER Groundwater Permit Compliance Records Groundwater Permit Correspondence D

Groundwater Permits & Applications HAZARDOUS WASTE Hazardous Waste Correspondence Hazardous Waste Inspection Records Hazardous Waste Permit & Applications Hazardous Waste Permit Compliance Records Mixed Waste Compliance Records Mixed Waste Correspondence Uniform Hazardous Waste Manifest (UHWM), Land Disposal Restriction Notification (LDRN) Records, and PCB Certificates of Disposals (CDs)

Waste Characterization & Laboratory Records NATIONAL HISTORIC PRESERVATION ACT (NHPA)

NHPA Compliance Records NHPA Correspondence NEPA Environmental Assessments-Administrative Records Environmental Impact Statements-Administrative PCB waste manifests and certificates of disposal are managed via the hazardous waste document type: "Uniform Hazardous Waste Manifests, Land Disposal Restriction Notification (LDRN) Records, PCB Certificates of Disposal (CDs)"

TVA 20946 [07-21-2014]

Page 1 of 2

ENVIRONMENTAL RECORDS PROCESSING FORM Records Non-Project NEPACorrespondence OIL SPILL PREVENTION & COUNTERMEASURES Certificates of Applicability of Substantial Harm Criteria Facility Response Plan Compliance Records Facility Response Plan Correspondence OilTransfer Manual Compliance Records Oil Transfer Manual Correspondence SPCC Correspondence SPCC Equipment Testing SPCC Plan Compliance Records SPCC Plans SPCC Spill Records PCBS*

PCB Annual Logs PCB Cleanup Records PCB Compliance Records PCB Correspondence PESTICIDES Pesticide Applicator Certifications - Contractor Pesticide Correspondence Pesticide Inventory Pesticide Use Compliance Records SARA TITLE III, EPCRA CERCLACorrespondence CERCLA Reasonable Care Evaluations Form R Toxic Release Inventory Supporting Documentation SARA Correspondence SOLIDWASTE (INCLUDES SPECIAL WASTE)

Solid Waste Correspondence SolidWaste Disposal Permits &Applications SolidWaste Disposal Compliance Records D

Other SolidWaste Compliance Records (Special Waste Analysis)

UNDERGROUND STORAGE TANKS (USTs)

UST Certification Forms & Certificates UST Closure UST Correspondence UST Financial Responsibility UST Manufacturer's Information for New USTs &

Monitoring Systems UST Monitoring &Calibration USED OIL Used Oil Correspondence Used Oil Sampling Results & Burn Records Used Oil Shipping Papers WATER/WASTEWATER Corps of Engineer PermitCorrespondence Corps of Engineer Permits &Applications Corps of Engineer Permit Compliance Records InjectionWell Permit Correspondence Injection Well Permits &Applications Injection Well Permit Compliance Records NationalPollutant Discharge Elimination System (NPDES) Correspondence NPDES Permits &Applications NPDES Permit Compliance Records Non-Project Water Compliance Sewage Treatment Compliance Records Sewage Treatment Correspondence Sewage Treatment Permits &Applications Storm Water Correspondence Storm Water Permit Compliance Records Storm Water Permits, Notices of Intent, &

Applications Tennessee Aquatic Resource Alteration Permit Compliance Record Tennessee Aquatic Resource Alteration Permit Correspondence Tennessee Aquatic Resource Alteration Permits &

Applications Water Quality Certification Compliance Records Water Quality Certification Correspondence Water Quality Certification Permits &Applications El GENERAL/OTHER NON-MEDIA SPECIFIC EMS Correspondence EMS Documents Environmental Event Reports (after Reporting to CDO and other required notifications)

Environmental Audit Correspondence Environmental Audit Reports Environmental Outlook Documents Environmental Performance Reports (Internal)

Environmental Performance Reports (External)

Regulatory Issue Summaries ERALAudit Reports ERALCorrespondence & Contracts External Correspondence on Proposed Regulations or Legislation Operational Control Procedures Self-Assessment Reports Utility Group Reports/Meeting Notes TVAEnvironmental Policy PCBwaste manifestsand certificates ofdisposalare managedviathe hazardouswaste documenttype: "Uniform HazardousWaste Manifests, LandDisposal Restriction Notification (LDRN) Records,PCBCertificates of Disposal (CDs)"

TVA 20946 [07-21-2014]

Page 2 of 2

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: November 09.2018

1. Facility / Discharger: Sequoyah Nuclear Plant / TVA 2.

County / State: Hamilton / Tennessee 3.

NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant 5.

Design Flow (MGD): L579 6.

Receiving Stream: Tennessee River (TRM 483.6) 7.

IQ10: 3,491 8.

Outfall Tested: ipj.

9.

Dates Sampled: October 07 - 12.2018

10. Average Flow on Days Sampled (MGD): 1748. 1891. 1740

11. Pertinent Site Conditions: Production / operation data will be provided upon request.

12. Test Dates: October 09-16. 2018

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephalespromelas)

Daphnids (Ceriodaphma dubia)

15. Concentrations Tested (%):

Pimephales promelas: UV treated Outfall 101: 10.7. 21.4. 42.8. 85.6. 100 UV treated Intake: 100 Ceriodaphmadubia: Non-treated Outfall 101: 10.7. 21.4. 42.8. 85.6. 100 Non-treated Intake: 100

16. Permit Limit Endpoint (%): Outfall 101: IC?s = 42.8%

17. Test Results: Outfall 101: Pimephales promelas: IC25> 100%

Ceriodaphma dubia: IC25 > 100%

Page 1 of91

18. Facility

Contact:

Millicent Garland Phone #: (423)843-6714

19. Consulting / Testing Lab: Environmental Testing Solutions* Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Donald W. Snodgrass Phone #: (256) 386-2787

22. Notes: Exposures to samples collected October 07 - 12, 2018 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >100 percent. Exposure ofminnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Page 2 of 91

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101. Intake 2.

Sample Type: Composite 3.

Sample Information:

Sample ID Date (MM-DD-YY)

Time (ET)

Collected Date (MM-DD-YY)

Time (ET)

Received Anival Temp.

(°C)

Initial TRC*

(mg/L)

Date (MM-DD-YY)

Time (ET)

Last Used By 101 10-07-18 0800 to 10-08-18 0700 10-08-18 1530 1.2

<0.10 10-09-18 1010 10-10-18 0923 Intake 10-07-18 0800 to 10-08-18 0700 10-08-18 1530 1.1

<0.10 10-09-18 1010 10-10-18 0923 101 10-09-18 0800 to 30-10-18 0700 10-10-18 1240 5.6

<0.10 10-11-18 0925 10-12-18 0915 Intake 10-09-18 0800 to 10-10-18 0700 10-10-18 1240 2.4

<0.10 10-11-18 0925 10-12-18 0915 101 10-11-18 0800 to 10-12-18 0700 10-12-18 1320 1.1, 1.7*

<0.10 10-13-18 0958 10-14-18 0939 10-15-18 0911 Intake 10-11-18 0800 to 10-12-18 0700 10-12-18 1320 1.9

<0.10 10-13-18 0958 10-14-18 0939 10-15-18 0911

TRC = Total Residual Chlorine

+Samples werecollected intwo 2.5gallon cubitainers. Temperature wasmeasured in eachcubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured bv Emperor Aquatics.

Inc.) for 2 minutes.

Page 3 of 91

Test Organisms:

1.

Source:

2.

Age:

Test Method Summary:

1.

Test Conditions:

2.

Test Duration:

Pimephales promelas In-house Cultures

< 24-hours old Static. Renewal 7 days 3.

Control / Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

4 5.

Organisms per Replicate: K)

6. Test Initiation: (Date/Time): 10-09-18 0827 ET 7.

Test Termination: (Date/Time): 10-16-18 Q732 ET 8.

Test Temperature: Outfall 101:

Mean = 24.6°C (24.2-25. TO Ceriodaphmadubia In-house Cultures

< 24-hours old Static, Renewal Until at least 60% ofcontrol females have 3 broods Moderately Hard Synthetic JLQ i

10-09-18 1010 ET 10-16-18 0920 ET Mean = 24.9°C (24.7~25.2°C) 9.

Physical / Chemical Measurements:

Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Page 4 of 91 Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McICinneyville. CA).

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1.

Results of a Pimephalespromelas Clironic/ 7-dav Toxicity Test.

(Genus species)

(Type / Duration)

Conducted October 09-16.2018 using effluent from Outfall 101.

Test Solutions

(% Effluent)

Percent Surviving (time interval used - days) 1 2

3 4

5 6

7

Control, UV-treated 100 100 100 100 100 100 100 10.7%

100 100 100 100 100 100 100 21.4%

100 100 100 100 100 100 100 42.8%

100 100 100 100 100 100 100 85.6%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100

Control, Non-treated 100 100 100 100 100 100 100 Test Solutions

(% Effluent)

Mean Dry Weight (mg)

(replicate number) i 2

3 4

Mean

Control, UV-treated 0.841 0.861 0.908 0.941 0.888 10.7%

0.749 0.876 0.936 0.890 0.863 21.4%

0.874 0.878 0.896 0.819 0.867 42.8%

0.878 0.927 0.981 0.866 0.913 85.6%

0.872 0.905 0.894 0.975 0.912 100.0%

0.766 0.909 0.913 0.952 0.885 Intake 0.972 0.903 0.847 0.815 0.884

Control, Non-treated 0.840 0.878 0.949 0.875 0.886 IC25 Value: > 100%

Permit Limit: 42.8%

95% Confidence Limits:

Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1 Permit Limit: 2.3 TUc

.0 TUc*

TUa = 100/LCso: TUc = 100/ IC25 Page 5 of 91

TOXICITY TEST RESULTS (seeAppendix C for Bench Sheets) 2.

Results ofa Ceriodaphniadubia Cluonic/ 7-day Toxicity Test.

(Genus species)

(Type/ Duration)

Conducted October 09-16. 2018 using effluent from Outfall 101.

Test Solutions

(% Effluent)

Percent Surviving (time interval used - days) 1 2

3 4

5 6

7 Control 100 100 100 100 100 100 100 10.7%

100 100 100 100 100 100 100 21.4%

100 100 100 100 100 100 100 42.8%

100 100 100 100 100 100 100 85.6%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Test Solutions

(% Effluent)

Reproduction (#young/female/7 days)

Data (replicate number) 1 2

3 4

5 6

7 8

9 10 Mean Control 29 31 34 28 27 32 27 31 33 32 30.4 10.7%

32 33 35 30 37 36 30 37 38 34.1 21.4%

34 39 35 35 34 36 35 39 23 35.3 42.8%

35 33 40 39 34 31 39 38 40 36 36.5 85.6%

35 36 41 37 40 34 37 41 37 38 37.6 100.0%

38 37 41 39 41 37 40 37 39 37 38.6 IC25 Value: > 100%

Permit Limit: 42.8%

95% Confidence Limits:

Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 2.3 TUc TUa = IOO/LC50: TUc = 100/ IC25 Page 6 of91

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2.

Results ofa Ceriodaphnia dubia Chronic/ 7-dav Toxicity Test.

(Genus species)

(Type / Duration)

Conducted October 09-16,2018 using water from Intake Test Solutions

(% Effluent)

Percent Surviving (time interval used - days) 1 2

3 4

5 6

7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions

(% Effluent)

Reproduction (#young/female/7 days)

Data (replicate number) 1 2

3 4

5 6

7 8

9 10 Mean Control 30 27 31 27 32 30 28 29 29 29 29.2 Intake 37 38 36 34 37 38 38 34 35 35 36.2 ICzs Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = IOO/LC50: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC25)

Pimephales promelas October 09-16, 2018 0816 7 days KC1 0.67 g/L Ceriodaphnia dubia October 09-16,2018 0947 7-days NaCl 1.06 g/L Page 7 of91

PItVSlCAUCUKMICAL

SUMMARY

Water Chemistry Mean Values and Ranges Ibr UV-treated Pimephales promelas and Non-treated Ceriodaphma dubia. Seqiioyuh Nuclear Plant (SQN). Effluent OmlaJI 101 and Intake performedOctober 09*16? 2018.

Test Sample ID Temperature (°C)

Dissolved Oxygen (mg/L) pit (S.D.)

Conductance

(/tmhos/em)

Alkalinity (mgflLCaCOj)

Hardness (mg/LC&COj)

Totnl Residual Chlorine (mg/L)

Initial Final Initial Final Initial Final i

1 Control.

Nan-fti't>>M 24.7 24.6 24,7 24.4 243 24.5 7.8 7 7 8.0 7.6 6.6 79 7.62 7,49 7.75 732 7.06 7.70 309 297 319 58 59 60 81 80 84 Control.

UV-itcitlrd 24.7 247 248 24.6 244 24.7 7.9 7.8 8.1 7.8 7.1 8.1 7.70 7.46 7.87 7,34 7 13 7.71 307 295 318 59 59 59 81 80 82 10.7%

24.8 247 24,9 24.5 24.3 246 8.0 7.9 8.1 7.7 7.0 8 1 7.69 748 7 87 7,30 709 769 294 276 314 21.4%

24.8 24 7 24.9 24.4 243 24.6 8.1 7.9 8,2 7.7 6 8 8.1 7.68 748 7 86 7.30 7.06 7.68 275 264 291 42.8%

24.8 24.7 25.0 24.5 243 24,6 8.1 80 8.2 7.7 6.7 H2 7.66 7.48 7.84 7.29 7.06 -

7.65 240 231 250 85.(i%

24.9 24.7 25.0 24.5 24,2 24.7 8.1 8.0 8.3 7.8 6.7 S.2 7 61 7.46 780 7.27 6.97 7.62 170 162 179 100%

24.9 24.8 250 24.4 24.2 247 8.2 8.1 8.3 7.8 6.8 8.2 7.58 7.44 7.79 7.21 6.96 7.59 149 137 160 50 53 54 53 52 54

<0,10

<0 10

<() to Intake 25.0 24,7 25.1 24.5 24.3 247 8.2 8.0 8.4 7.8 6,7 8,3 7.55 7.43 7.80 7 17 6.92 7.53 143 135 158 52 53 56 55 52 58

<0J0

<0 10

< 0.1.0

,<<2 ft

Couirol, Noimrented 24.7 24.7 248 25.0 248 25,2 7,8 7.7 7.9 7.9 7.8 8.0 7.62 7,49 7.75 7.64 7,40 7.82 309 297 319 58 59 60 81 80 84 10.7%

24.8 24.7 249 24.9 248 25,1 7,9 7 7 8.1 8,0 7.9 S.l 7.73 7.50 7.86 7.63 74!

7.81 291 279 308 21.4%

24.8 24.7 24 9 24.9 24.8 25,1 7.9 77 81 8.0 7.9 8.1 7J0 7.48 7.83 7.62 7.42 7.80 272 262 285 42.8%

24.8 247 25.0 24.9 24.7 25.1 8.0 7,7 8.2 8.0 8 0 8.1 7.66 745 781 7.60 7 41 7.79 239 232 249 85.6%

24.9 24.8 25.0 25.0 248 25,2 8.0 7.8 8.2 8.1 7 9 8.1 7.58 739 775 7.57 7.40 -

7.75 170 160 177 100%

24.9 247 25.0 24.9 24.8 25.0 8.1 7.8 8.2 8.1 7.8 8.2 7.54 7.36 7.73 7-55 7.35 7.74 142 134 148 50 52 54 53 50 54

<0.10

< 0 10

<0.I0 intake 24.9 24.7 25.0 24.9 248 25, t 8.1 7.8 8.3 8.1 7.8 8.3 7.53 736 776 7.53 732 7.74 141 134 148 52 53 56 55 52 58

<0.IO

<0.10

Nolc. Total residual chlorine was performed onnon-trcaicd Outfall 101 and Intake samples Overall1cninenilure(°C)

Pimephalespromelas Ceriodaphnia dubia Average (VI minium Maxinmm 24.6 24.2 25!

24.9 24.7 25.2

SUMMARY

/ CONCLUSIONS-Exposuresto samples collectedOctober07 - 12,2018 from. Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >100 percent Exposureof minnowsand daphnids to intake samples resulted in no significantdifference from the controlsduring this study period.

Page 9 of 91

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of500-mL plastic disposable cups, each containing 250-mL oftest solution.

2.

Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL oftest solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL 1.

Pimephales promelas Samples used in the fathead minnow test were exposed to UV light for two minutes prior to introduction oftest organisms. UV treatment is used to control interference offish pathogens. This treatment method was approved on November 23, 2015 by the State of Tennessee in a letter from Jessica Murphy to Terry Cheek, Senior Manager of TVA Water Permits, Compliance, and Monitoring.

2.

Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS 1.

Pimephales promelas None 2.

Ceriodaphnia dubia None Page 10 of 91

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: Allchemicals were certified products used before expimtion dates (where applicable).

2.

Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B-2010.

4.

Dissolved oxygen was measured by SM 4500-O G-201L

5. The pH was measured by SM 4500-H+ B-2011.

6.

Conductance was measured by SM 2510 B-2011.

7.

Alkalinity was measured by SM 2320 B-2011.

8.

Total hardness was measured by SM 2340 C-2011.

9.

Total residual chlorine was measured by ORION 97-70-1977.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCl.

2.

Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3.

Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 11 of 91

REFERENCES 1.

NPDES Permit No. 1TM0026450.

2.

USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms. EPA-821-R-02-013 (October 2002).

j>.

Standard Methods for the Examination of Water and Wastewater. 22ndEdition. 2012.

4.

Quality Assurance Program: Standard Operating Procedures. Environmental Testing Solutions, Inc (most current version).

Page 12 of 91

Sequoyah Nuclear Plant Biomonitoring October 09-16.2018 Appendix B Diffuser Discharge Concentrations ofTotal Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 13 of 91

Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L mg/L TRC TRC Phosphate Copolymer DMAD Azole Quat mg/L EO/PO Quat 02/06/2005

<0.0042 0.028 0.010 02/07/2005

<0.0116 0.028 0.010 0.007 02/08/2005

<0.0080 0.028 0.010 02/09/2005 0.0199 0.028 0.010 02/10/2005

<0.0042 0.028 0.010 02/11/2005 0.0155 0.028 0.010 0.007 06/05/2005 0.0063 06/06/2005 0.0043 0.037 06/07/2005 0.0103 0.037 06/08/2005 0.0295 0.037 06/09/2005 0.0129 06/10/2005 0.0184 07/17/2005 0.0109 0.026 0.009 07/18/2005 0.0150 0.026 0.009 0.036 07/19/2005 0.0163 0.026 0.009 0.036 07/20/2005 0.0209 0.026 0.009 0.014 0.036 07/21/2005 0.0242 0.026 0.009 07/22/2005 0.0238 0.054 0.018 0.014 10/30/2005 0.0068 10/31/2005 0.0112 11/01/2005 0.0104 0.035 11/02/2005 0.0104 0 036 11/03/2005 0.0117 0.036 11/04/2005 0.0165 0.035 11/14/2005 0.0274 11/15/2005 0.0256

11/16/2005 0.0234 11/17/2005 0.0231 11/18/2005 0.0200 11/19/2005 0.0116 Page 14 of 91

Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M MSW Hypochlorite mg/L mg/L mg/L mg£L mg/L mg/L 73551 mgft 101 mg/L TRC Phosphate Copolymer DMA© Azole Quat mg/L

<$uat mg/L TRC EO/PO Phosphate 11/12/2006 0.0055

., V.:">

11/13/2006 0.0068

- daS7 11/14/2006 0.0143 0.037 11/15/2006 0.0068 0,037 11/16/2006 0.0267

"0:837 11/17/2006 0.0222

?

11/26/2006 0.0188 11/27/2006

'* r 0.0138 V

11/28/2006 0.0120 r.

11/29/2006 0.0288 11/30/2006 0.0376 12/01/2006 0.0187 05/28/07 0.015 05/29/07

&036 0.015 05/30/07 0.0084 0.017 oteo^s 0.015 05/31/07 0.0103 0.036 0.015 06/01/07 0.0164 0.017 0.036 0.015 06/02/07 0.0305 0.015 12/02/07 0.0241

'^

12/03/07 0.0128

12/04/07 0.0238

^

- '*w \\'

12/05/07 0.0158 12/06/07 0.0162 12/07/07 0.0175 04/13/08 0.0039 04/14/08 0.0124 04/15/08 0.0229 04/16/08 0.0143 04/17/08 0.0120 04/18/08 0.0149 10/26/08 0.0260 10/27/08 0.0151 0.017 10/28/08 0.0172 0.041 10/29/08 0.0154 0.018 0.041 0.030 10/30/08 0.041 0.030 10/31/08 0.0086 0.041 0.030 Page 15 of 91

Date Sodium Towerbrom PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L

-PF mg/L mg/L 73551 CT1300 mg/L 101 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg/L Quat mg/L TRC Phosph ate EO/PO Quat Phosphate 02/08/09 0.0197 0.017 02/09/09 0.0237 0.017 02/10/09 0.0104 0.021 02/11/09 0.0155 0.017 02/12/09 0.0106 0.017 02/13/09 05/10/09 0.0129 05/11/09 0.0415 0.0446 05/12/09 0.0053 0.0396 05/13/09 0.0049 0.0396 05/14/09

<0.0141 0.0397 05/15/09

<0.0160 11/15/09 0.025 11/16/09 0.0152 11/17/09 0.0255 11/18/09 0.0306 11/19/09 0.0204 11/20/09 0.0093 05/09/10 0.0192 05/10/10 0.0055 05/11/10 0.0100 0.039 05/12/10 0.0171 0.039 05/13/10 0.0041 0.039 05/14/10 0.0099 0.039 Page 16 of 91

Date Sodium Towerbrom PGL-PCL-401 GL-363 Cuprostat msoM Nalco Spectrus H-150M W&1&. Floguard Hypo mg/L 222 mg/L mg/L

-PF mg/L mg/L 73551 CT1300 mg/L l;lll;:,l MS6236 chlorite TRC mg/L Copoly DMAD Azole Qtfat mg/L mg/L Quat Jitflfc; mg/L mg/L Phos mer EO/PO Quat l^ffi Phosphate TRC phate 10/31/10

7f:*'%:;

11/01/10 0.0122 M-m--**'*

11/02/10

.N-0.0112 lllll:-;;..'-^

11/03/10 0.0163 jK'*7-'/

11/04/10 0.0107

--M+ V '*

11/05/10 0.0132

/* -'

WM-&:

05/01/2011 05/02/2011

0.04

]^^;*.:J.

05/03/2011 0.04 mm^-:

05/04/2011 0.0155 0.04 V'?:***.*'"

05/05/2011 0.0179 0.04 V-j,, V.-y/;]

05/06/2011 0.0089 wmm 11/06/2011 0.0168 V:^:~\\

11/07/2011 0.0225

'*::>%- -* I 11/08/2011

-~

0.0141

'^ff§*.y-^;J 11/09/2011 0.0239 KHJ;;

11/10/2011 0.0242

^&'-:?.

,' C 11/11/2011 0.0231 05/06/2012 05/07/2012

^fev^v'-Vi 05/08/2012

~

0.041 lil/JSl:iR.J;'^

05/09/2012 0.0145 0.041

\\:S^\\:y&^'['*'*;.]

05/10/2012 0.0298 0.041

^&4^\\^

05/11/2012 0.0174 llil';:l 08/12/2012

^*0&*

0.029 08/13/2012 0.0256 0.028 0.037 t^,f:'-:^:-

0.029 08/14/2012 0.0209 0.037 lififet.-.^

0.029 08/15/2012 0.0279 0.028

'W:'j*

0.029 08/16/2012 0.0076 r~:~y~::. \\. :*

0.029 08/17/2012

'i 0.0446 IfliSS 0.032 05/12/2013 0.0099

'}lWk-:'**:'*.

05/13/2013 3.^:y-^';" '**/*/

0.064 05/14/2013 0.0091 0.039 IXSl#45^

0.064 05/15/2013 0.0096 0.039

?'V: ^:i.'.: *'*:

0.064 05/16/2013 0.0229

^;.i- -

0.032 05/17/2013 0.0063 illl;:,.;:£ 0.032 09/15/2013 R?I;'^:--';'**!**

0.03 09/16/2013 0.0072 0.0379

v/;-';

m .*':

0.03 09/17/2013

~

0.0107 0.036 0.03^9 tM0£/y \\;:{r 0.03 09/18/2013 0.0217 0.036 010379 Wfp+/- -***;*:

0.03 09/19/2013 0.0172

\\ -"-;'

0.03 09/20/2013 0.0173

\\

W^-s 0.03 Page 17 of 91

Date Sodium Towerbrom PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Floguard Hypo mg/L 222 mg/L mg/L

-PF mg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly DMAD Azole Quat mg/L mg/L Quat mg/L mg/L mg/L Phos mer EO/PO Quat Phos Phosphate TRC phate phate 05/04/2014 0.0118 05/05/2014 0.0112 05/06/2014 0.0096 05/07/2014 0.0164 05/08/2014 0.0235 05/09/2014 0.0110 09/07/2014 09/08/2014

0.04 09/09/2014 0.04 09/10/2014 0.04 09/11/2014 0.0070 09/12/2014 0.0074 08/09/2015 08/10/2015 0.0195 0.03 08/11/2015 0.0275 0.03 08/12/2015 0.0213 0.03 08/13/2015 0.0192 0.03 08/14/2015 0.0182 0.03 10/18/2015 0.0162 10/19/2015 0.0125 10/20/2015 0.0120 10/21/2015 0.0130 10/22/2015 0.0174 10/23/2015 0.0156 05/15/2016 05/16/2016 0.0209 05/17/2016 0.0210 05/18/2016 0.0361 05/19/2016 0.0254 05/20/2016 0.0261 07/31/2016 08/01/2016 0.0091 0.03 08/02/2016 0.0093 0.03 08/03/2016 0.0209 0.03 08/04/2016 08/05/2016 04/30/2017 05/01/2017 0.0298 05/02/2017 0.0218 05/03/2017 0.0260 05/04/2017 05/05/2017 Page 18 of 91

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Page 20 of 91 Sequoyah NuclearPlant Bioraonitoring October 09-16,2018 Appendix C Chain ofCustody Records and ToxicityTest BenchSheets

Client: TVA 4>

roject Name: Sequoyah NP Toxicity XX-

6.0. Number
N/A 3ti
<o facility Sampled: Sequoyah NP NPDES Number: TN0026450 Collected &j j/u A£lW> &bU*Ht Avv)h tk*W**
a BIOMONITORING CHAIN OF CUSTODY RECORD Page 1
of 1_
Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 Fax:
828-350-9368 Delivered By (Circle One):
FedEx UPS Bus Client^
Other (specify): CbnWcW General Comments:
101 Bottle Comp 0*6$° INT Bottle Comp @0?°$
Field identification /
Sample Description Grab/Comp Collection Date/Time Container Number &
Volume Collected Flow (MGD)
Rain Event?
(Mark as Appropriate)
Laboratory Use SQN-I01-TOX Comp SQM-INT-TOX Comp Date (mro/dd/yy)
Start lO-I'VZ End iJ-£- \\$
Start 10-">-<*
End lot- [$
Relinquished By (Signature):
-tvft
]Jg£§fr iaj
Sw* ST
^e>M TVIfv CDtJXftACS>>*_
Timu (EST) 0?oo OlOO o
0$° I (2.5gal)
P%$m BTS Log Number 10IO06.0Z
\\Bmd.o3 Arrival Temp, By f.a c
^
Ire
^
Time
/S30
/S>o Appear ance Q7QO 1 (2.5 gal)
>>/(\\
-f Custom\\
SeAti u>r*cr. Siv^'ues Sample Custody-Fill In From Top Down Date/Time Received By (Signature):
Date/Time iQ'f-t?/ \\\\\\S 6.1-lo-r-ir / infer 10-^'i? / JrJ^sr
££&&} \\Cfifc>M*l
/0-OS-tS /SSo er
s CD O
Whole Effluent Sample Receipt Log Page H^
Sample temperature performed usingSampleReceiving Thermometer: SN160928622 Date Received Time Received Received by Received from
Sample Temp. (DC)
Project number Sample number Sample name and description State Comments 10-08-18 1200 K, Keenan W.Ladd 3.4 13632 181008.01 Andrews WWTP - Pp NC 10-08-18 1530 K. Keenan A. Panter 1.2 13633 181008.02 TVA - Sequoyah Nuclear Plant -101 TN 10-08-18 1530 K, Keenan A. Panter 1.1 13633 181008.03 TVA - Sequoyah Nuclear Plant ** Intake TN SOP G4 - Exhibit 64.2, revision 01-03-12
^jient: TVA
-0) roject Name: Sequoyah NP Toxicity g.0. Number: N/A facility Sampled: Sequoyah NP NPDES Number: TN0026450 Coiled*, Mb *&"£<- J&>^^
BIOMONITORING CHAIN OF CUSTODY RECORD Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 Fax:
828-350-9368 Delivered By (Circle One):
FedEx UPS Bus Other (specify): £on*C General Comments:
101 Bottle Comp @CH&5 INT Bottle Comp @ cn^
Page 1
of 1_
Client Field Identification /
Sample Description Grab/Comp Collection Date/Time Container Number &
Volume Collected Flow (MGD)
Rain Event?
(Mark as Appropriate)
^
Laboratory Use Sample Custody-Fill In FromTop Down Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time
\\oi\\M 3 of&*£R$&L
£0>>&C *be<<~*<<4H
/o^/
o%'33*x tffl J££j2^
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/0-fO-<t ix^o *-T
<o rirftt Whole Effluent Sample Receipt Log Page 1Z1
Sample temperature performed using Sample Receiving Thermomeierr SN 160928622 Date Received Time Received Received by Received from Sample Temp. (*C)
Project 1
number Sample number Sample name and description State Comments 10-10-18 0931 K.Keenan Fed-Ex 1.8 13645 181010.01 ALCOA -005 NC 10-10-18 0931 K.Keenan Fed-Ex 1.8 13646 181010.02 ALCOA-012 NC 10-10-18 0931 K. Keenan Fed-Ex 1.8 13647 181010.03 PCSPhosphate, Inc NC 10-10-18 1042 K, Keenan Fed-Ex 1,6 13648 181010.04 Carv/ApexWTF NC 10-10-18 1042 K. Keenan Fed-Ex 2.4 13649 181010.05 Ctarkton WWTP NC 10-10-18 1042 K. Keenan Fed - Ex 2,4 13650 181010,06 Cherry Point NC 10-10-18 1042 K. Keenan Fed - Ex 1.4 13651 181010,07 Ounn WWTP NC 10-10-18 1042 K. Keenan Fed - Ex 2.3 13652 181010.OS f armville WWTP NC 10-1048 1042 K. Keenan Fed - Ex 2.4 13653 181010.09 Frankllnton WTP NC 10-10-18 1042 K. Keenan Fed - Ex 2.4 13654 181010.10 Hamlet WWTP NC 10-10-18 1042 K. Keenan Fed - Ex 2.2 13655 181010.11 Greenville WTP NC 10-10-18 1042 K. Keenan Fed-Ex 1.2 13656 181010.12 GoldsborcWRF-001 NC 10-10-18 1042 K. Keenan Fed-Ex 2.4 13657 181010.13 Hermitage House WWTP m
10-10-18 1042 K. Keenan Fed-Ex 1.7 13658 181010.14 Marshall SS NC 10-10-18 1042 It Keenan Fed-Ex 1.4 13659 181010.IS Mayodan WWTP NC 10-10-18 1042 K. Keenan Fed-Ex 2.4 13660 181010.16 Mt. Olive WWTP NC 10-10-18 1042 K. Keenan Fed-Ex 1.4 13661 181010.17 Newport WWTP NC 10-10-18 1042 K. Keenan Fed - Ex 2.2 13662 181010,18 Pasquotank WTP NC 10-10-18 1042 K. Keenan Fed-Ex 2.4 13663 181010,19 Princeton WWTP NC 10-10-18 1042 K. Keenan Fed-Ex 1.6 13664 181010,20 Radiator Specialty NC 10-10-18 1042 K. Keenan Fed - Ex 1.7 13665 181010.21 M'KeanMaffitWWTP NC 10-10-18 1042 K. Keenan Fed - Ex 2.4 13666 181010.22 Smlthfleld Packing NC 10-10-18 1042 K.Keenan Fed - Ex 2.8 13667 181010 23 South Mills WTP NC 10-10-18 1042 K.Keenan Fed - Ex 1.5 13668 181010.24 Greenville WWTP NC 10-10-18 1042 K.Keenan Fed-Ex 2.4 13669 181010.25 Bay Valley Foods NC 10-10-18 1042 K.Keenan Fed - Ex 1.6 13670 181010.26 Mt. Olive PickleCompany NC 10-10-18 1042 K.Keenan Fed-Ex 2.4 13671 181010.27 Worsley NC 10-10*18 1042 K.Keenan Fed-Ex 1.1 13672 181010.28 TVA-Lagoon Greek CC-Outfall 001 TN 10-10-18 1042 K.Keenan Fed - Ex 1.3 13672 181010.29 TVA - Lagoon Creek CC-Well Water TN 10-10-18 1109 K, Keenan W. Ladd 2.0 13632 181010.30 Andrews WWTP -Pp NC 10-10-18 1240 J. Sumner TVA Courier 5.6 13633 181010,31 TV/p-Sequoyah Nuclear Plant -101 TN 10-10-18 1240 J. Sumner TVA Courier 2.4 13633 181010.32 TVA - Sequoyah" Nuclear Plant-Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12
<o Date Received 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 40-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10-12-18 10X12-18 10-12-18 Time Received 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 0951 1250 1320 1320 Received by K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan K. Keenan J. Sumner J. Sumner Received from Fed-Ex Fed-Ex Fed - Ex Fed - Ex Fed -Ex Fed - Ex Fed -Ex Fed - Ex Fed-Ex Fed - Ex Fed-Ex Fed-Ex Fed -Ex Fed-Ex Fed-Ex Fed-Ex Fed -Ex Fed-Ex Fed-Ex Fed - Ex Fed - Ex W.Ladd TVA Courier TVA Courier Whole Effluent Sample Receipt Log Page H/S
'"Sample Temp.fC) 1.6 2.0 2.0 2.7 2.4 1.6 1.6 2.4 2.1 2.0 1.9 2.5 2.0 3.5 2.0 2.2 1.8 2.0 2.0 1.7 1.4 0.6 1.1/1,7 1.9 Project number 13648 13649 13650 13651 13652 13653 13654 13655 13656 13657 13658 13659 13660 13661 13663 13664 13665 13666 13669 13670 13668 13632 13633 13633 Sample number 181012.01 181012.02 181012.03 181012.04 181012.05 181012.06 181012.07 181012.08 181012.09 181012.10 181012.11 181012.12 181012.13 181012.14 181012.15 181012.16 181012.17 181012.18 181012 J 9 181012.20 181012.21 181012.22 181012.23 181012.24
Sampletemperature performedusingSampleReceiving Thermometer: SW 160928622 Sample name and description State Comments Cary/Apex WTF ClarktonWWTP Cherry Point Dunn WWTP Farmville WWTP Frankllnton WTP Hamlet WWTP Greenville WTP GoldsboroWRF-001 Hermitage House WWTP Marshall SS Mayodan WWTP Mt Olive WWTP Newport WWTP Princeton WWTP Radiator Specialty M'KeanMaffitWWTP Smlthfleld Packing Bay Valley Foods Mt. Olive Pickle Company Greenville WWTP Andrews WWTP -Pp TVA -Sequoyah Nuclear Plant -101 TVA - Sequoyah Nuclear Plant - Intake NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC NC HC NC NC NC NC TN TN SOP G4 - Exhibit G4.2, revision 01-03-12
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Sequoyah Nuclear Plant NPDES #: TN0026450 Project*:
rSfalft County: Hamilton Outfall: 101 Page I of7 Dilution preparation information:
Comments:
Dilutionprep (%)
10.7 21.4 42.8 85.6 100 Each concentration was UV-treated Effluent volume (mL) 267.5 535 1070 2140 2500 for 2 minutes to remove pathogenic Diluent volume (mL) 2232,5 1965 1430 360 0
Interferences.
Total volume (mt) 2500 2500 2500 2500 2500 Test organism information:
Test information:
Organism source:
In-house culture Randomizing template:
kuoe-Age:
< 24-hours old Incubator number and shelf location:
1C Spawn date; W-CrArlfc Anemia CHM number CHM984 Hatch dates and times:
""TO I0-OV14 QtoQO Drying informationfor weight determination:
Transfer vessel information:
pH=
a so S.U.
Temperature = lH>\\
°C Date / Time in oven:
16-l.lrl*
<n4S Initial oven temperature:
feG-C Average transfer volume:
< 0.25 mL Date / Time out of oven:
I5>>t1~t(
OlM£ Final oven temperature:
urc Total drying time:
1H-AOA&
Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Time Analyst Time Analyst Test initiation, renewal, or termination Time Analyst Sample numbers used Outfall 101 Intake MHSW batch used 10-09-18 ObuS 4-y\\Q%
of^"^
\\g /Oftf-CO.
WlOOif-Ck (00W 10-10-18 QfaOQ QJD0 OSi\\
X
\\&to Otf-gL lgfOOf-fifr fo-<S-tf IO-il-18 tdSfr*,**
10-12-18 mo
_QfetO_
oftiS ixfo QtTL-
IV-lJHOiD^\\
lg*0i&*VL.
4-itioio/b'x i&ioib.Si io-<rvil io-ovig 10-13-18 CTTDCi
^
W>0 0^'T- ¥<*
\\%\\x>\\\\. xs>
ICIDIX-XA lo-U-if ^
10-14-18 Cfloo
\\>>>o&
£
<HV\\
uipq-is
\\gtDtt.vt tD*v\\-lf A 10-15-18 QloOO 4-rLift 04 tl
£tt> n,-13>
iSlotl.ttt
b-H-ft£A KM6-18 cftM--
1
&*T Control information: *jH&d*
OV*~ TtUATCfc Acceptance criteria Summary oftest endpoints:
% Mortality:
07.
<20%
7-day LCso
> I0&1.
Average weight per initial larvae:
o.&SS-NOEC 1007.
Average weight per surviving larvae:
0. 6&S
> 0.25ma/larvae LOEC
>iooi ChV
>I007.
1C25
>i0O7.
Page 27 of 91 SOP AT20 - Exhibit AT20.3, revision II-0I-I4
\\rrnz=m^
3C nut tnCK^i(Uinx^
Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, UV-treated Page 2 of7 Date: 10-09-18 Survival and Growth Data Day l
CONTROL 1
10.7%
21.4%
A B
c O 1 E F
G l
H I
j K
L 0
10 10 10 10 10 10 10 10 10 10 10 10 1
Ifc i0 (0
ID
/D ID
<D
/o
/o
'6
'0
'0 2
(Q 10
>>0 10 10
>>0 ID to 10
'0
<<0
/o 3
10 ID 10
£0 10 to to to
'0
<D ID I 10 4
'D 10 10 ID to ID to ID 10 to (Q
to 5
ID 10 ID 10 10 10 to to ID
'0 to to 6
Id 10
<0 lO to to 10
<<o to to (Q
to 7
/o fb
'0
'0 fO ib
/o to 70
/o 10
'0 A = Pan weight (mg)
Tray color code::
fokk/
Analyst:
TS Date:
Qft.3fo-tt 15.90 13.35 /5J7 /<</.H0 15.<<
)H.n 13.7(3 15.7? X53\\ I3.S6 13.66 13.77 B << Pan + Larvae weight (mg)
Analyst:
A(
Date:
f&*t"Vi&
7rt.ii 1\\A^ *>>WS 2*S\\ 11.^ ivM ^^.cfe T£V\\ ii\\M w.rt 1-L.Sfe 26.%
C = Larvae weight (rag) ~ B - A Hand calculated.
Analyst:
&Mi S.bV
<\\M VU Vtt
&.1U V<<" lAO
&M sag
&.<Uo g.*4 Weight per initial number of larvae (nig)
= C / Initial number of larvae Hand calculated.
Analyst:
\\ v s*
0#
0*
0" 0*
dv o
1v Average weight per initial number of larvae (mg)
Percent reduction from control (%)
o.US.
c-j^.
0.8v>>*>>
2-&7.
o.&tn 7.m.
Commentcodes: c = clear, d = dead, fg = fungus, k - killed, m - missing* sk =
Ig = unusually large, d&r - decanted and returned, w = wounded.
Comments:
sick, sm = unusually small, Page 28 of 91 SOP AT20- Exhibit AT20.3, revision 11-0I-I4
Pa*e 3 of 7 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant OutfaU 101, UV-treated Date: 10-09-18 Survival and Growth Data Day 42.8%
85.6%
100%
M N
O p
Q R
S T
U V
w X
0 10 10 10 10 10 10 10 10 10 10 10 10 I
ffc
<b 10 to ib to to to to to to ID 2
<<D (0
10 to 10 to ID to
'0
'0
/o 10 3
>>b 10 10 10
'0 1Q 10 to to 10 10 to 4
10 10 10 J0 10 to 10 to to to 10 to 5
ID 10 10 (O
10 10 to lO tb 10 to
>>0 6
to
'0
>>0 10 to
<<0 to to to 10 10
'0 7
lO 10
/e>
lb to lb
/O
/o to to to to A << Pan weight (mg)
Traycolor code::
fOo^
Analyst:
TS Date:
6&Xt-l%
15.00 15-75 15M \\5m 15.73 IW IH.86 \\&n [5.U 11.7;? ISM 15.TS B - Pan + Larvae weight (mg)
Analyst:
X\\
Date:
^H-H-l*
7>>m Ti\\.*fl ts.<& TH-lfc ulM* TA& i%&b 2s.m v^s Ti.Sl TM.il TS.S0 C = Larvae weight (mg)= B - A Hand calculated.
Analyst:
\\\\
.i&
%r\\
<uv a.wi
&.~u SM
<\\.m
-i.u>>
I.Crt
°>>.>>b
i.-si.
Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculated.
Analyst:
Y\\
J9 O'
0' 0'
0' OS?
Average weight per initial number of larvae (mg)
Percent reduction from control (%)
0.4'^h "2.4 7.
0.<=\\\\TL
-1.17.
o.s&s 0.2>7.
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
Page 29 of 91 SOP AT20 - Exhibit AT20.3, revision 11-0I-I4
Paae4of7 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant Outfall 101, UV-treated Date: 10-09-18 Survival and Growth Data Day 100% Intake l
Control-Non-treated Y
z AA BB l CC DD EE FF GG HH n
jj 0
10 10 10 10 10 10 10 10 1
Ib to to to
0
'0 to
'0 2
10 10 10
'0
>D
'0
/o to 3
10 10 to 10 10 to to to 4
/o to to 10
'0
<<0
'0 tD 5
to
<<0 10 to 10 10 10 to 6
/o ID 10
'0 to to to 10 7
/o to
'0
/o
/O
/o to
/o A = Pan weight (mg)
Tray color code::
f0 D*j Analyst:
TS Date:
0$. d&-)%
15.11 \\5M R5I m.w I3.S? 15.51 aw l<<U8 B = Pan+ Larvae weight/mg)
Analyst:
\\f Date:
U-rV< S v\\M
un 21.1& x"&.oS 2<<.VL -tj.*\\ 7S.U w/>>&
C= Larvae weight (mg)= B-A Hand calculated.
Analyst:
JV Wl
<\\.<r*> i^n
&.*S 8.MO g.->>g
^.w e.nS Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculated.
Analyst:
M 0*
0' 0*
o'
(^
0" 0'
U f}\\
Average weight per initial number of larvae (mg)
Percent reduction from control (%)
o.&H O.H7.
O.a^o J^4 Comment codes: c = clear, d = dead, fg= fungus, k = killed, m= missing, sk = sick,sm= unusually small, Ig = unusually large, d&r = decanted and returned, w - wounded.
Comments:
Page 30 of 91 SOP AT20 - Exhibit AT20.3, revision 11-01-14
to TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16,2018 Pimephales promelas Chronic Whole Efflucnl ToxicityTest EPA-82l-R-l)2-013, Method 1000.0 QuMlltyControl Verification c<<r Diita Entry, Calculations, and Siuiixiienl Aniilvse<.
Environmental Testing Solutions, Inc.
Project uumuen 13039 I.'MI l.l! ' '.jll'l i i'l ' '
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HW LmvL'f.irnlvipjivr j'MSD bounds were (!cK'tmtin;i! NornHi-' t'iili iukH0j& petecmfta, hsjicclively. ofPMJiO.Inl.ifioifi i:PA'? WUT (nlwrltiUeratisy V.miliiliiy Study (USItPA, iiKJI.i. US!:!'A lw\\hi nClwqnu: amiAeweWhttkEfllitaitH faKMHQ r<<<< ^fcthwtft, V'oft>>uei 18nd3rAp]i<<ari<< iiPArffai-B-Oi-OOJ andEPA-ttai-S-QI-iX)
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Jrftww,
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J Cnvlronmfnul'fimifigSoJuUwKtnc.
Start Date-10/9/2018 End Date 10/16/2018 Sample Date: October 2018 Comments:
UV Treated Conc-%
TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16,2018 Statistical Analyses Larval Fish Growth and Survival Tost-7 Day Growth Test ID' PpFRCR Sample ID:
Lab ID ETS-Envir Testing Sol.
Sample Type.
Protocol: FWCHR-6PA-821-R-Q2-013 Test Species; TVA / SON 101 DMR-Discharge Monitoring Report PP-Ptmephales promelas Non-Control 0.8400 08780 09490 0.8760 UV-COntrol 0.8410 0.8610 0.9030 0.9410 10 7 0.7490 0.8760 0 9360 08900 214 0,8740 0 8780 08960 0 8190 42.8 0,8780 0,9270 0,9810 0,8660 85.6 08720 0,9050 0.8940 09750 100 0 7660 0 9090 0.9130 0.9520 intake 09720 0.9030 0.8470 0.8150 Moan N-Moan Transform i Untransformod t-Stat 1-Tailed Critical MSD fsot<
Moan
>nlc Conc-%
Moan Min Max cv%
N N-Mean Non-Control 08855 0.9976 0.8855 0.8400 0.9490 5.162 4
UV-COntrol 0.8876 1.0000 0 8878 0.8410 0.9410 5 099 4
08884 1.0000 107 08628 0.9718 08628 0.7490 0.9360 9.278 4
0 598 2410 0.1007 0.8884 10000 21.4 0,8668 0.9763 0.8668 0.8190 0.8960 3.835 4
0.502 2.410 0 1007 0 8884 1.0000 42.8 0.9130 1.0284 0.9130 0.8660 0.9810 5.745 4
-0 604 2.410 0.1007 0.8884 1.0000 856 0.9115 1.0268 0 9115 0.8720 0.9750 4.882 4
-0 568 2.410 0 1007 0.8864 lijooo 100 0.8850 0.9969 0 8850 0.7660 0.9520 9.228 4
0.066 2.410 0.1007 0.8S50 0.9962 Intake 0.8843 0.9961 08843 0.8150 0.9720 7.790 4
Auxiliary Tosts Statistic Critical Skow Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01)
Btertlett's Test indicates equal variances fp = 0.66)
The control means are not significantly different (p >> 0 95) 0.93304 3.2891 006995 0.884 15.0863 244691
-06932 0.22375 Hypothesis Tost (1-tail, 0.05}
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetfs Test Treatments vs UV-COntrol 100
>100 1
0.10073 0 11347 0.00182 0.00349 0.75724 5, 18 Point SD Unaar Interpolation (200 Rosamplos) 96% CL(Exp)
Skow tcos MOO IC10 MOO IC15
>100 (C20
>100 IC25 MOO IC40
>100 IC50 MOO Doso-Rosponso Plot Page 32 of 91 50 100 Do-so %
1-taii. 0.05 level of significance fr
TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16,2018 Statistical Analyses Environmental Testing SotutlonK Inc.
Intake Larval Fish Growth and Survival Test-7 Day Growth Start Date:
End Date:
Sample Date:
Comments:
10/9/2018 10/16/2018 October 2018 UV Treated Test ID:
PpFRCR Sample ID:
Lab ID:
ETS-Envir. Testing Sol Sample Type:
Protocol: FWCHR-EPA-821 -R-02-013 Test Species-TVA/SON 101 -Intake DMR-Discharge Monitoring Report PP-Pimephales promelas Conc-%
Non-Control UV-COntrol 10.7 21,4 42.8 85.6 100 Intake 0.8400 08410 0.7490 0.8740 0.8780 0.8720 0.7660 0.9720 0.8780 0.8610 0.8760 0.8780 0.9270 0.9050 0.9090 0.9030 0 9490 0.9080 0.9360 0.8960 0.9810 0.8940 0.9130 0.8470 0.8750 0.9410 0.8900 0.8190 0.8660 0.9750 0.9520 0.8150 Transform: Untransformed Conc-%
Mean N-Mean Mean Non-Control 0.8855 0.9975 0.8855 0.8878 0.8628 0.8668 0.9130 0.9115 0.8850 0.8843 UV-COntrol 10.7 21.4 42.8 85.6 100 Intake 0.8878 0.8628 0.8668 0.9130 0.9115 0.8850 0.8843 1 0000 0.9718 0.9763 1.0284 1.0268 0.9969 0.9961 Min 0.8400 0.8410 0.7490 0.8190 0.8660 0.8720 0.7660 0.8150 Max 0.9490 0.9410 0.9360 0.8980 0.9810 0.9750 0.9520 0.9720 CV%
5.162 5.099 9.278 3.835 5.745 4.882 9.228 7.790 N
4 4
4 4
4 4
4 4
t-Stat 1-Tailed Critical MSD 0.085 1.943 0.0801 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0 01)
F-Test indicates equal variances (p = 0.51)
The control means are not significantly different (p ~ 0.95) 0.95112 2.31582 0.06995 0.749 47.4683 2.44691 0.40793
-0.9433 Hypothesis Test (1 -tail, 0.05)
MSDu MSDp MSB MSB F-Prob df Homoscedastic t Test indicates no significant differences Treatments vs UV-COntrol 0.08008 0.09021 2.5E-05 0.0034 0.93508 1,6 Dose-Response Plot 1.2 Page 33 of 91 1-tatl, 0.05 level of significance
I r>3 ET^O fp
>£ Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant Outfall 101, UV-treated Daify Chemistry:
Page 5 of7 Date:
1Q-Q9-18 Day (Analyst identified for each day, performed pH, P.O. and conductivity measurements only.)
Analyst ML Concentration Parameter CONTROL UV-treated 10.7%
21.4%
42.8%
85.6%
100%
100%
Intake pH (S.U.)
DO (mg/L)
Conductivity (ujuhos/cm)
AlkaJinity (mgCaCOs/L)
llnrdness (mgCaCCVL)
^Temperature (°C) pH (S.U.)
DO (mg/L)
Conductivity (umhos/cm)
^Temperature (°C) pH (S.U.)
DO (mg/L)
Conductivity (umhos/cm)
^Temperature (°C) pH (S.U.)
DO (mg/L)
Conductivity (umhos/cm)
^Temperature (°C) pH (S.U,)
DO (mg/L)
Conductivity
(^mhos/cm)
^Temperature (°C) pH(S.U.)
DO (mg/L)
Conductivity (umhos/cm)
^Alkalinity (mgCaCQt/L)
Hardness (mgCaCOs/L)
TR chlorine (mg/L)
^Temperature (°C) pH (S.U.)
DO (mg/L)
Conductivity (umhos/cm)
^Alkalinity (mgCaCQs/L)
^Hardness (mgCaCOj/L)
TR chlorine (mg/L)
^Temperature (°C)
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^Temperatures performed at Ute lime oftest initiation,renewal or tenninationby the analyst identifiedin the Daily Renewal Information tablelocatedon Page 1.
ilinity, Ijardncss and total residual chlorine pcrfonncd bytheanalyst identified onthebench sheet specific for each analysis and transcribed tothisbench sheet Total residualchlorine was performedon non-treatedOutfall 101 and Intakesamples.
£ Page 34 of 91 SOP AT20 - Exhibit AT20.3, revision 11-0I-14
IFF1
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Page 6 of 7 Species: Pimephales promelas Client: TVA/Seauovah Nuclear Plant, Outfall 101, UV-treated Date:
10-09-18 Analyst Day (Analyst identified foreachday, performed pH, D.O.and conductivitymeasurements only.)
3 4
5 6
W^8 l5f&0 ft?t-T3 TS HS
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Concen tration Parameter
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9.1 7-5 Ao S3 Conductivity (umhos/cm) 344
,

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^Temperature (°C)
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inperatures performed at tlie timeof test initiation,renewalor terminationbythe analystidentifiedin the DailyRenewal Information tablelocatedon Page I.
inity, hardness and totalresidual chlorineperformed by theanalystidentified on the benchsheetspecific foreach analysisand transcribed to thisbenchsheet
\\A. Total residual chlorine was pcrfonncd on non-treated Outfall 101 and Intake samples.
SOP AT20 - Exhibit AT20.3, revision 11-01-14 Page 35 of 91
T fr!
Species: Pimephales promelas lJO,>>Tflj&dVrfc Client: TVA /Sequoyah Nuclear Plant, Outfall 101,<<V'ti'cakd^^
Page 7 of7 Date: 10-09-18 Daily Chemistry:
Day (Analyst identified for each day, performed pH, P.O. and conductivity measurements onlvJ 0
1 2
Analyst HJ MS HJ T5
<<9*V yu Concentration Parameter
'SZgfflShZJA *SB?r;r£i;i fr<<Coi-.':rzi3 Control Non-treated pH (S.U.)
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Initial Final Initial Final l
Initial Final l
Temperatures performed atthetimeof testinitiation, renewal ortennination bytheanalyst identified intlieDaily Renewal Information table located on Page 1.
Alkalinity hardness andtotal residual chlorine performed by theanalyst identified onthebench sheetspecificfor eachanalysis andtranscribed to thisbench sheet by: ^_-
Page 36 of 91 SOP AT20 - Exhibit AT20.3, revision l l-Ol-M
T Environment] Ibttlng Solution*, inc.
TVA /Sequoyah Nuclear Plant, Outfall 101, UV-treated October 09-16, 2018 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-82I-R-02-0I3, Method 1000.0 Daily Chemical Analyses ProjCCl nijti'.lirt:
13633 Concentration Parameter l)n\\ O 1>:iv 1
Day 2 Day J Daj 4 Day 5 Day 6 Initial Final Initial Phial tnlttal Pinal Initial 1 illlfl initial Final Initial Pinal Initial Final
Control, Noo-lrcntcil lil MNi:)
7 CO 7 70 7.75 7 52 7 50 7 17 7 61 7 10 7 5n
<i 7.70 7.28 7.40 7 06 DO (mg/L) 7.7 78 7,8 19 so 6.6 7.7 7 5 7 s 7 0 7.7 77 7 7 7 0 Conductivity {untbos/cm]l 519 316 302 50(1 207 313 313
?\\ll(;iliniiy [mg/L CaCOJ 60 50 58 Hardness [mg/L CnCOj)
S4 XO 30 U'iiilicr:iiui'i' ("C"l 24 6 24 4 74.7 24 4 34 ??
24 3 24 7 24 5 24
?*
24 5 24 7 24.4 74 7 24.3 Controti UV-treaCcd I.1KSIU 7 70 7 71 7 S 7 750 7 SI 7 15
? 69 7.13 7<J!
. 42 7 70 7.46 7 IS DO(taeM 7 0 7 7, 7.0 7.8 XI 7 1 7 X s
1 g.0 X 1 x.o 7 0 7 0 8 0 Conductivityfumhos/cm) 305 311 205 300 205
ix J 17 Alknltnit) (mg/L CaCOj) 59 JO 5"
Hardness(tngCL CnCOj)
S2 so XII TTeatpefStttre ("Cl 2-1.7 24.6 24 X 24 7 24 7 24.5 24.X 24 0 24 7 24.5 24.8
24. g 24,7 24 4 10.7%
pll(SC) 7,74 7 60 7.83 7 4;-:
7',
7.11 7 60 7 60 7 60 7.37 740 7.25 7 48 7 14 IKHinti/l.s 8 1!
7.6 10 7.5 X 1 7.0 7?/
? s S.I SI so 7 7 7 0 S.I Conductivity (tunhos/crn) 77o 4i4 501 292 280 298 300 1cmncraittFC ('Cl 24.8 34 5 24 0 24 5 3.4.7 24 n 24 0 24 5 24 X 24 5 24.S 24 4
17 24 4 2 1.4%
Dlt(SU) 7.73
?.

7 so 7 45 7 77 7 12 7.60 7 06
'*?
7.38 7.64 7 27 7
IS 7 00 DOOM",'!.)
S.I "T
"J B 1 7 8 X3 6.8 7 0 X 1 X.I X !
SO ClitKlUf tivitV (lIImIk>s/CI111 267 201 269 7 77 204 381 YIA l\\'ii:[K'r.i!ufv? f "Cl
24. 8 24 6 24.0 24 5
24 7 24 3 24 9 24 5 24.X 2.4 S 24 4 24 7 24,5 42.8%
pIKSU) 7 71 7.65 7 84 7 48 7 72 12 7 68 7.00 7 55 7.37 7 35 7 48 7.07 HOlme/I.)
S.I 7 7
,5.2 7.8 X 2 6.7 so 7 X 8,1 x 7 s
1 78 S 0 X 7 Conductivity (tunhas/cm) 234
.750 234 244 231
!4E 24 7 Tt'mitcnitnrt' CO 24 S 2-i.O 25.0 24.!>
24,7 24 6 24 0 24 5 24,8 24 5 24 $
24 5 24 S 24.3 ns.fr/,.
ilLK.St!)
764 7iO 7 SO 7.44 7 65 7.12 1 64 7 II 7,49 7,35 7 56 7 27 7 40 6.07 DO(mft/L)
S.I 7 8
5 2
o S3 6.7 SO 7
7 S.I X 2 X2 7 8 X 1 S.2 Conductivity (pmiios/cm) us 179
[62 171 166 I7S 167 Temperature ( ( 1 24.0 34 5 25 0 74 (I 24 7 24 2 25 0 24.4 24.S 24 24 8 24 7 24 8 24 6 100%
iiHtsSi 7 61 7 59 7 78 7 4?1 7 60 7 (? 1 706 7.46 7.27 7 53 7 18 7 14 6 96 l)()(m>>/L) 8 1
s si 3
,' *j 5 5 6 X S
7h S.I 8 2 8
7 S 8 1 3.2 CmttliK-livitv (umhos/t'iul 142 157 157 145 146 160 155 Alltalmiiy (m<</CCaCO,)
54 Si?
54 Hardness (niK/LCsiO).,)
54 52 54
1"t?il Residual Chlorine (mc/L
'0. 10 0 10
().10 l emncratorc ((')
35.0 74 7 25.0 34 6 24 S 24.5 25.0 24 2 24 O 24 2 51,8 24 1
24 0 24 5 100% Intake n'Hfsui 7 S3 7 53 7X0 7 56 7 54 6 00 7.61 6 06 7 43 7 28 I 40 7 13 7.45 6532 pOung/L]
?*;
i 7 8 8.3 8.0 8.4 0 7 82 7 0 SO S3 Q 7 7.0 8
1 82 Cotnhicfivhv (jiiuhos/cml 142 158 135 14 2 155 I4X 142 AJknlinilj (mg/L CaCOj) 56 52 52 Hardness lm<<<l CnCO.,)
58 52 54
" I <?l:il Ki'Mdnal Chlorine (miOl, 0,10 n in
0 10 Temiterutnrc ("CO 25.0 24 7 25 l 24 3
>> 0 24 0
25. 1 24.5 25 0 M
4 24 7
!4 7 24 8 34 V
' Ncrc. Vital resldu 1chlorine was performed on non-1 eattd Ouilhl 101 ami Oil ike samples File: sqn10i_10Q918chem Entered by T Steeper Reviewed by v\\.
ilSf, K.
Page I of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia Client: Tennessee Valley Authority Facility: Sequoyah Nuclear Plant NPDES #: TN00264S0 Project#:
ftfcgfo County: Hamilton Outfall: 101 Dilutionpreparation information:
Comments:
Dilution prep(%)
10.7 21.4 42.8 85.6 100 Effluentvolume (mL) 267.5 535 1070 2140 2500 Diluent volume (mL) 2232.5 1965 1430 360 0
Totalvolume (mL) 2500 2500 2500 2500 2500 Testorganism source information:
Test information:
Organism age:
Date andtimes organisms werebom between:
<24-hoursold lO'OVlf" OW*> *ro 0<V%S Randomizing template color incubator number and shelf location:
Culture board:
Replicate number:
Culture board cup number:
!O>>01<<l.i A
^o-n-iEft YWT batch:
Transfer vessel information 3b M3 UK H
lo \\%m
-^~^.
y,,
x.
Average transfer volume(mL):
j <Q,25mL Daily renewal information:
pH<<,"\\..&SS>>a Temperature" iAA°C Seietmstnmt batch:
Day Date 10-09-18 10 IS 10-11-18 10-12-18 10-13-18 10-14-18 10-15-18 10-16-18 Test initiation and feeding, i MHSW renewal and feeding, or l batch used termination time IDIO 10-CVU 0^03 IQ-OV^
oW^
io>>tn-\\i o<^S iQ-ovtg o*\\sfr KMMfiA o<V^
lildkliA.
6<\\\\\\
Q<<\\H&r\\
(WUb Sample numbers used Outfall 101 Intake
\\ iJPOg.Ql
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O-0Wf Analyst 4-
-4 ii
A A
4t il Control information:
Control-!
ControI-2 Summary oftest endpoints:
% of Male Adults:
% Adults having 3°* Broods:
% Mortality:
Mean Offspring/Female:
% CV:
Page 38 of 91 01.
JO&7 07.
3o-H 8.a7.
07.
>>007.
07.
T.Cy.1.
S-S7.
Acceptance criteria
£20%
£ 60% surviving adults 520%
£ 15.0orTspring/female
<42.0%
7-day LC50 NOEC LOEC ChV IC25
> 1QQ7, 1067*
> *OQ7.
>*OQ7.
>tOD7.
SOP ATI l - Exhibit ATI i.2T revision 11-01-14
immsm
,,' t<<*n>R>><<ta*ITti<<(vj$rfmlcnVin Page2 of7 Species: Ceriodaphma dubia Client: TVA / Scquovah Nuclear Plant, Outfall 101 CONTROL-1 Survival and Reproduction Data Date: 10-09-18 Replicate number Day 1
2 3
4 5
6 7
8 9
10 l
Young produced 0
D 0
O 0
o 0
0 0
O Adult mortality v_
V
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o Adult mortality
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0 O
o Adult mortality VU
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v_
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>>4 in v2i
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t<~~>4 i$~~
~~iS Total young produced Vk 2>\\~~
~~34 T-g XT "bt-7.1 s\\~~
~~Si 31-Finnl Adult Mortality v_~~
v_
\\
v_
\\
\\__
V_
v_
\\
\\
X for 3rd Broods 2S, Y~
?<-
^
?fr
>C
>^
>c
?<- 7^
Matt; Adult *nortafity it = live,D* dead).JJB<< spfitbro<yd(singlebro3dsplitbetweentwo days), C6 ** carry over(GflVprins>carried overwlJiadult during transfer)
Concentration:
% Mortality:
07.
Mean Offspring/Female:
2)0.4 CONC: 10.7%
Survival and Reproduction Data Day Replicate number 1
2 3
4 5
6 7
8 9
10 I
Young produced ft 0
0 0
0 0
0 O
o O
Adult mortality v^
\\_-
V_
v^.
u.
v_
v_
\\-
>^.
c_
2 Young produced O
o o
0 n
O o
o o
0 Adult mortality v_
v_
L_
v_
\\^
v_
V l_
L_
L.
3 Young produced e>
0 0
o 0
O 0
0 c
b Adult mortality u.
u_
v__
^
\\
V_
w
^_
l_
L_
4 Young produced 4
s M
M s
4
^>>
s s
S Adult mortality u
\\^_
\\_
v_
v__
v_
v^
v^
v^.
5 Young produced IT-
'^
^
>(
'D i^S l?i
<<o IT-
>T_
Adult mortality V_
V v__
\\^
u_
L_
t^.
\\__
V_
V-
<j Young produced O
0 O
6 O
O 0
0 0
O Adult mortality
\\_
v_
v_
L_
\\
u v_
\\_
L^
7 Young produced l\\o l^>

l

1_ ^2>> 3S 3*. 3b ^>n iW 3>>fe 3ft 3S Final AdultMortality .U V-v^. V_ v_ v_ \\ v_ l_ L_ A'ow: AdultmortalUy (L = five.D >> dead),SB=split brood(>>nsJebroodspfitbct\\>,cen hvo da)*s>. CO - carryover(offspringcarried overwithadultduringtransfer) Concentration: % Mortality: 07. Mean Offspring/Female: M.\\ % Reduction From Control-1: -tt. X 7. Page 39 of 91 SOP ATI I - Exhibit ATI 1.2, revision 1l-Oi-14 lPp-j csp3 rzzj i:~ . tj<<wiHvn>>itufTtisJisgSefcMk>>ilnc Page 3 of? Species: Ceriodtwhnia dubia Client: TVA / Sequoyah Nuclear Plant Outfall 101 Date: 10-09-18 conc: 21.4% Survival andReproduction Data Day Replicate number 10 Young produced Adult mortality _Q_ O _£l Jl .a sl v_ SL Young produced Adult mortality .Cl U -Cl _D_ _0_ JL £L £L v^ Young produced Adult mortality _Q _Q O SL Sl o O o O Young produced Adult mortality H +/-L <A ^ +/-L Young produced Adult mortality l"l_ V_ lb \\i_ il i^S '^ t o Young produced Adult mortality v_ v_ Sl O o o Young produced Total young produced 11 ^2> \\& v* 7.0 h°\\ -to
s ig iS A

~~3M 3\\o 14 3>>S X\\ 2>>1 t*\\ 32> Finn! Adult Mortality ^ Ate Adiilt rooitafiiy (L* live, D*dead). SB*>> split brood (angle btood spUt bemtseq hvo days). CO~~

~~carry over(otBpring carried over with adult during transfer).~~

Concentration: % Mortality: 07. Mean OfTspring/Fetnaie: 3S.5 % Reduction from Control-1: - It. \\T, CONC: 42.8% Survival andReproduction Data Day Replicate number 1 2 3 4 5 6 7 s 9 10 1 Young produced r> O O 0 0 0 o o o Adult mortality u v_ \\^ L_ L. l_ l_ u U L_ 2 Young produced o 0 C) 0 0 C\\ 0 ft O Q Adult mortality i_ l_ L_ \\_ v_ v_ L_ l_ U 3 Young produced o o o 0 £> O O a 0 o Adult mortality v_ v__ v_- v_ U. V \\- u~ V. 4 Young produced 4 4 \\* v* 4 4 ^> s u s Adult mortality v_ \\_ v__ v_ v_ v_ \\_ u. v_ v_ 5 Young produced i4 \\T_ ^ <<-b >>?> u >>3> i4 ii IV Adult mortality v^ s_ \\_. V ^ ^_ \\ ^_ <^- 6 Young produced rs 0 O 6 D O 0 O 0 o Adult mortality ^ \\_ L_ \\-_ L_ u_ \\_ ^. ^ 7 Young produced n n T-\\ xo n "r-t\\ x<\\ "Li -LG Total young produced 3S 2>i 4t> 2rt

\\

~~3\\~~

~~<\\~~

~~3S Mt> 3t Final Adult Mortality V- \\ \\^_ v_ v_ v_ v^_ 1_~~

~

Vw_ AVwe: Adult mortality (L >>live, D>>dead), SB <<split brood(single brood split betweentwo days). CO<<canyovwjojggrm^ Page 40 of 91 Concentration: % Mortality: 07. Mean OfTspring/Fernale: ilo.S % Reduction from Control-1: -"io.\\7. SOP ATI I -Exhibit AT! 1.2, revision l l-Ol-M 3 I t t>2 i IrrHiaMtHflUfTcTttr^SctuttaA&tec. Page4 of? Species: Ceriodaphnia dubia Client: TVA / Sequoyah Nuclear Plant, Outfall 101 Date: 10-09-18 conc: 85.6% Survival and Reproduction Data Day Replicate number 8 10 Young produced Adult mortality XL O O H ^ o SL O Young produced Adult mortality L_- G D_ SL 0 SL u. u. SL Young produced Adult mortality O D o O O _0_ O Young produced 4 Adult mortality Young produced Adult mortality l*t_ v4 v_

"b

~~<<4 \\x.~~

b

~~\\b j>L IT \\A Young produced O O Adult mortality Young produced Total young produced \\& rt 2>S 31= T-T. m \\i 3-V Tl Md V\\ TO 34 art TL ^\\ TO~~

1

~~\\<\\ Final Adult Mortality Note: Adultmortality (L~~

~~live.D<< dead), SB - splitbrood(single broodsplitbetween two days). CO* carry over(otTspring carried overwithadultduring, transfer).~~

Concentration: % Mortality: 07. Mean Offspring/Female: 2n.< % Reduction from Control-1: -iyn. CONC: 100% Survival and Reproduction Data Day Replicate number I 2 3 4 5 6 7 8 9 10 1 Young produced O O O 0 0 O 6 0 O 0 Adult mortality v_ \\_ V_ L. i_ l_ v-~ v*_ V, t_ 2 Young produced n 0 A a 0 0 0 0 0 0 Adult mortality u u. l_ v_ u v__ \\ l-_ i_ l-3 Young produced o 0 O o O O 0 0 0 Q Adult mortality ^ v v_ \\ L_ v__ I ^ 4 Young produced s s U u s 4 i<< s s "A Adult mortality V. \\^_ v_ I v v_ <w 5 Young produced <<vi ^ K >>4 vS IT_ i4 VT_ \\1_ >>T_ Adult mortality V-u v_ v_ L_ i_ v_ v_ i^_ w_ 6 Young produced 6 o Ci b O 0 0 r> 0 o Adult mortality V_ u_ L_ l_ L_ u v^. \\_ L_ ^ 7 Young produced i<\\ i<\\ Tl \\\\ M l-l T-to TO T.T-VS Total young produced M VI <-U TA 4\\ n Ht> an <<i<V v\\ Final Adult Mortality V V. \\_ v-1v_ V-v_ v_ \\^ V-A'o/<f.- Adultmortality (L <<live. D = dead).SB = splitbrood(sinsle brccd spUt betweenlwt> da;*). CO*3 carry©vcr (offspring carriedover with adult during transfer). Concentration: % Mortality: 0?. IvleanOfTspring/Femaie: 2&.fa tVo Reduction from Control-1: -2101. Page 41 of 91 SOP ATI I - Exhibit ATI 1.2, revision 11-01-14 Page 5 of7 Species: Ceriodaphma dubia Client: TVA /Sequoyah Nuclear Plant Outfall 101 Date: 10-09-18 CONTROL-2 SurvivalandReproductionData Day Replicate number 10 Young produced Adult mortality SL v_ v_ JQ_ O o SL Vv. D jCl Young produced Adult mortality £ Q. ^SL SL u~ \\^_ n. o _Q_ ^ Young produced o O Adult mortality SL £LQ_ _D_ .0_ Young produced Adult mortality J^L ^_ 4 Young produced Adult mortality to V-ID v_

~~o IS, 11~~

>>o IT lO \\\\ Young produced Adult mortality XX v_- D Young produced Total young produced Final Adult Mortality X for 3rd Broods Ho 2*o 5£ \\^ TTt TT ^T .4 2>>\\ Tn v-^ i+/- "bT, lid 30 T* l\\ U\\ is n JX^. v* IfK y-I >- I 5gr A'ote Adult roortaBly (L=live. D* dead). SB* split brood (single brood split bctivccn Kmdays), CO *cany over(offspring carried over>vith adult during transfer). X*. Concentration: % Mortality: 07. Mean Offspring/Female: T^.T CONC: 100% Intake Survival andReproduction Data Day Replicatenumber l 1 2 3 4 s c 7 8 9 10 1 1 Young produced n 0 D o o 0 0 O O rt Adult mortality \\_ v_ v_ L_ c_ u. \\- V_ L_ 2 Young produced o O ft ft 0 ft 0 0 0 D Adult mortality L_ \\_ V-. \\_ v_ L_ v_ l_ 3 Young produced O o X) O 0 O 6 o o o Adult mortality ^_ L_ v L_ v_ *

~~j L_~~

v^ ^ v>_ 4 Young produced ^ u u S M lo n H ^ S Adult mortality \\~ v_ \\^_ V_ L_ \\ u_ v_ v_ v_ 5 Young produced i^b IT. VT. IV IT l-b IC. >>3> IT. >>^> Adult mortality \\_ \\ \\_ V_ t v^_ v_ v_ <^_ 6 Young produced O o o o O 0 O 6 O 0 Adult mortality v_ L- \\_ l^ L_ L_ ^. L_ i_ l~ 7 Young produced l6* T.O >>f IS Tl H Tl n ii n Total young produced si M 3t art sn 3& 3& a4 3S 36 Final Adult Mortality i_ V_ i_ l. i_ \\- i_ >~ Ateftv Adult inortality0- ~ five;0

~~dead),SB =spfitbrocwJ (single broodspfitbetween twodays). CO^carrvo~~

( 1 ( ver(offspringcarriedover withadult duringtransfer). Concentration: %Mortality: 07. Wean Offspring/Female: Aia' L-Va Reduction From Control-2: "2M.0?. Page 42 of 91 SOP ATI l - Exhibit ATI 1.2, revision 11-01-14 <o TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16, 2018 ~j f 5 Verification of Ceriodaphnia Reproduction Totals EnWionroefltaiTeitlny Solution), lite Control-I Day Replicate number Totnl 1 2 3 4 S 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 () 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 5 5 5 3 4 5 4 43 5 II 13 13 10 10 12 10 12 10 13 IN 6 0 0 0 0 0 0 0 0 0 0 0 7 14 14 17 13 12 15 14 15 IS 15 147 Total 29 31 34 28 27 32 27 31 33 32 304 85.6% t>ay Replicate rummer Total I 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 6 5 7 4 4 5 5 5 51 5 12 14 13 14 12 13 13 14 12 14 131 6 0 0 0 0 0 0 0 0 0 0 0 7 IS 17 22 IS 21 17 20 22 20 19 !94 Iota! 35 36 41 37 40 34 37 41 37 38 376 10.7% 100% Day Replicate number Totnl Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 I 2 3 4 § 6 7 8 9 10 1 0 0 0 0 0 0 fl 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 i 0 0 0 0 0 0 () 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 4 5 4 6 s 5 s 47 4 5 5 A 6 5 4 6 5 5 7 54 S 12 13 13 11 10 13 13 10 12 12 119 5 14 13 14 14 15 12 14 12 12 12 132 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 15 IS IS 15 20 17 15 20 21 175 7 19 19 21 19 21 21 20 20 22 (8 200 Total 32 33 35 33 30 37 36 30 37 38 341 Total 38 37 41 39 41 37 40 37 39 37 386 21.4% Control-2 Day Replicate number Total Day Replicate number Total 1 3 4 5 6 7 8 9 ill 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 6 5 5 4 6 4 5 4 47 4 4 3 5 s 5 3 3 6 4 4 12 5 12 12 13 10 12 11 12 13 13 10 118 5 10 10 12 10 13 II 10 12 10 11 109 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 18 20 20 18 19 18 IS 21 19 188 7 16 It 14 12 14 16 15 II 15 14 141 Total 33 34 39 35 35 34 36 35 39 33 353 Total 30 27 31 27 32 30 28 29 29 29 292 42.8% 100% Intake Day Replicate number Total Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 i 2 3 4 5 6 7 8 9 to I 0 0 0 0 0 0 0 0 0 0 0 J 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 6 6 4 4 5 5 6 5 49 4 5 6 6 5 4 6 7 4 4 5 52 5 14 12 13 13 13 II 13 14 13 11 127 5 13 12 12 11 12 13 10 13 12 13 121 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 i) 0 t) 0 (1 0 0 7 17 17 21 20 17 16 21 19 21 20 189 7 19 20 18 18 21 19 21 17 19 17 t89 Total 35 33 40 39 34 31 39 38 40 36 365 Total 37 38 36 34 37 38 38 34 35 35 362 TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16, 2018 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Environmental Testing Solutions, Inc. Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project1 number: 13633 Concentration (%) Replicate number Survival (%) Average reproduction (offspring/fcrmilc) Coefficient of variation {%) Percent rcitiictton from pooled controls (%) 1 2 3 4 5 6 7 8 9 10 Control - 1 29 31 34 28 27 32 27 31 33 32 100 30.4 8.2 No! applicable 10.7% 32 33 35 33 30 37 36 30 37 38 100 34.1 8.6 -12.2 21.4% 33 34 39 35 35 34 36 35 39 33 100 35.3 6.) -16.1 42.8% 35 33 40 39 34 31 39 38 40 36 100 36.5 8.7 -20.1 85.6% 35 36 41 37 40 34 37 41 37 38 100 37.6 M -23.7 100% 38 37 41 39 41 37 40 37 39 37 100 38.6 4.3 -27.0 Control -2 30 27 31 27 32 30 28 29 29 29 100 29.2 5.5 Not applicable 100% Intake 37 38 36 34 37 38 38 34 35 35 100 36.2 4.5 -24.0 Outfall 101: Duniicit's MSD vjtluc: PMSD: Intake: Dunnett's MSD value: PiMSD: 2.576 8.5 1.256 4J MSD = Minimum Significant Difference PMSD = PercentMinimum Significant Difference PMSD is ameasure oftest precision The PMSD is the minimum percent difference between the control and treatment Unit can be declared statistically significant inn wholeeffluenttoxicity test. Lower PMSD bound determined byUSEPA (10* percentile) <<* 13%. Upper PMSD bound determined byUSKPA (90* percentile) =47% Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from KPA's Win Intcrlqboratprv Variability Study(USEPA,200liv. USEPA,2001b). USIiPA 2001a, 2U0IK Final Report. Inlcrlnbormory Variability Study ofl-PA Short-term Chronic mid Acute Whole Hffluent Toxicity Test Methods. Volume* Iand 2-AppendiN l-PA-821-13*01-004 and FPA-8">l-lM)M)OS US linvironmcnial Protection Agency, Cincinnati,011 TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16, 2018 Envtienmtntat 1<MUi*9Sctattwtv tnc. Start Dale. End Dale: Sample Date: Comments. 10/9/2018 10/16/2018 October 2018 Non-treated Conc-% Control-1 Control-2 10.7 21.4 42.8 85.6 100 intake 29.000 30.000 32.000 33.000 35.000 35.000 38.000 37.000 31.000 27 000 33.000 34 000 33.000 36.000 37 000 38.000 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Test ID: CdFRCR Sample ID: Lab ID ETS-Envif. Testing Sol. Sample Type Protocol: FWCHR-EPA-821-R-02-G13 Test Species: TVA / SQN 101 DMR-Discharge Monitoring Report CD-Geftodaphnia dubia 34.000 31.000 35.000 39.000 40.000 41.000 41.000 36.000 28.000 27.000 33.000 35.000 39.000 37.000 39.000 34.000 27.000 32.000 30.000 35.000 34.000 40.000 41.000 37.000 6 32.000 30 000 37 000 34.000 31.000 34.000 37.000 38.000 Transform: Untransformod 27,000 28.000 36 000 36.000 39.000 37.000 40.000 38.000 8 31.000 29.000 30.000 35.000 38.000 41.000 37.000 34.000 33.000 29.000 37.000 39.000 40.000 37.000 39,000 35.000 10 32.000 29.000 38.000 33.000 36.000 38.000 37.000 35.000 Conc-% Mean N>>Mean Moan Min Max CV% 1-Tailsd t-Stat Critical MSD Isotonic Moan N-Moan 35.417 1.0000 2.576 35.417 1.0000 2.576 35.417 1.0000 2 576 35.417 1.0000 2576 35417 1.0000 2.576 35 417 1.0000 Control-1 Control-2 10.7 21.4 42.8 85 6 100 Intake 30.400 29.200 34.100 35.300 36.500 37.600 38.600 36.200 1.0411 1,0000 1.1676 1 2089 1 2500 12877 1.3219 1.2397 30.400 29 200 34100 35.300 36.500 37.600 38.600 36.200 27.000 34-000 27.000 32.000 30.000 38.000 33.000 39000 31.000 40.000 34.000 41000 37.000 41000 34.000 38.000 8.235 5.546 8.572 6 127 8688 6.417 4.266 4,473 10 10 10 10 10 10 10 10 -3.284 2.287 -4.349 2.287 -5.414 2.287 -6.391 2.287 -7.278 2.287 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) Bartlett's Test indicates equal vanances

100 1 2.57619 0.08474 85.9767 6.3463 1.4E-08 5.54 Point SD Linear Interpolation (200 Rosamplos) 96% CL Skew 1C05 >100 IC10 MOO IC15 >100 IC20 MOO IC25 MOO IC40 >10Q IC50 MOO Dose-Response Plot Page 45 of 91 50 100 Dose % 1-taii 0 05 level of significance TVA / Sequoyah Nuclear Plant, Outfall 101 October 09-16,2018 Intake Statistical Analyses Environmental Testing Solution*, Inc. Start Date: End Date: Sample Date: Comments. 10/9/2018 10/16/2018 October 2018 Non-treated Ceriodaphnia Survival and Reproduction Test-Reproduction Test ID: CdFRCR Sample ID: Lab ID: ETS-Envir. Testing Sol. Sample Type: Protocol: FWCHR-EPA-821-R-02-013 Test Species: TVA/SQN 101 - Intake DMR-Discharge Monitoring Report CD-Certodaphnia dubia Conc-% Control-1 Control-2 10.7 21.4 42.8 85.6 100 Intake Conc-% Control-1 Control-2 10.7 21.4 42.8 85.6 100 Intake 29.000 30.000 32.000 33.000 35.000 35.000 38.000 37.000 31.000 27.000 33.000 34.000 33.000 36.000 37.000 38.000 34.000 31.000 35.000 39.000 40.000 41.000 41.000 36.000 28.000 27.000 33.000 35.000 39.000 37.000 39.000 34.000 27.000 32.000 30.000 35.000 34.000 40.000 41.000 37.000 6 32.000 30.000 37.000 34.000 31.000 34.000 37.000 38.000 Transform: Untransformed Mean N-Mean Mean 30.400 29.200 34.100 35300 36.500 37.600 36.600 36.200 1.0411 1.0000 1.1678 1.2089 1.2500 1.2877 1.3219 1.2397 30.400 29.200 34.100 35.300 36.500 37.600 38.600 36.200 Min 27.000 27.000 30.000 33.000 31.000 34.000 37.000 34.000 Max 34.000 32.000 38.000 39.000 40.000 41.000 41.000 38.000 CV% 8.235 5.546 8.572 6.127 8.688 6.417 4.266 4.473 27.000 28.000 36.000 36.000 39.000 37.000 40.000 38.000 10 10 10 10 10 10 10 10 8 31.000 29.000 30.000 35.000 38.000 41,000 37.000 34.000 33.000 29,000 37.000 39.000 40.000 37.000 39.000 35000 t-Stat 1-Tailed Critical 10 32.000 29.000 38.000 33.000 35.000 38.000 37.000 35.000 MSD -9.666 1.734 1.256 Auxiliary Tests Statistic Critical Skew Kurt Shaptro-Wilk's Test indicates normal distribution (p > 0.01) F-Test indicates equal variances (p = 1.00) The control means are not significantly different (p = 0.22) 0.92285 1 1.27279 0.868 6.54109 2.10092 -0.0072 -1.1393 Hypothesis Test (1*tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences Treatments vs Control-2 1.25578 0.04301 245 2.62222 1.5E-08 1, 18 Dose-Response Plot Page 46 of 91 1-tail. 0.05 level of significance ?!'-; I 3 trtwrawvnulUtangietaOatA. Inc. Species: Ceriodaphnia dubia Client: TVA / Sequoyah Nuclear Plant OutfaU 101 Page 6 of7 Date: 10-09-18 Day (Analyst identified for each day*performed pH, D.O. and conductivity measurements only.) Analyst 0 1 2 fQ MS. HS T3 TS r° Concentration Parameter 'tell' ~rx~X~£'~ i" ?* ?"<; j". "* -. S3 £7&',i.-:2 CONTROL pH (S.U.) ~7.&<? ^.ez 7.:fcr
7. gO 7.5?

~~^.S0 DO (mg/L) 7-7 7.9 7.S 7.9 "7.^ Ofl Conductivity (jimhos/cm) 3/<? 3)0> 305~~

~~Alkalinity (mgCaCOa/L)~~

~~UO V ^<<t~~

~~Hardncss (mgCaCOs/L)~~

~~£<<A >?\\ ':>//f4ji':/yl" 80 ^Temperature (°C) tH-t -^.v "bV* T.S.X Ti^.l -U4.-8 10.7% pH(S.U)~~

~~7.qt, 7-#i 7-ff/i,~~

~~-7.20 7.7? a,<<s6 DO (mg/L) 7.1 &o 7-er 7.9 T:? a^> Conductivity (umhos/cm) Z8o 30S9 ^if-'^ir.'^ ^79~~

~~.'.-""F'.wv"".-~~
~~Temperature (°C)~~
~~vl-S~~

~~( "LS.\\ ->>M~~

~~\\£/D vi.-\\~~
~~v\\.f 21.4%~~

~~pH (S.U.) 7*3 TJgto 7.(33 T.79 7.K '*^n-DO (mg/L) l?-*? 7.9 7.<<>> T:^ 'w Conductivity (umhos/cm) Z&H 5 :.ji'~:C~'.'& -ZQs-~~

~~'" "**'.' "-'-1'- j ^3~~

~~.* *^"ft"^'<<*<^i*:.* w'~ ^Temperature (°C)~~

~~MK-&~~
~~^AA~~
vM

-vH-i TA-T TA-S 42.8% pH (S.U.) ^.sn 7.79 7.S">> 77f -Z6? ^-^ DO (mg/L) ^9 £>> 7.*? &o ~7.<r ao Conductivity (^mhos/era) w .r-it^riv V>>;'t. 24*? %>Z >4ilS^'>fel,.*.' ^Temperature (°C) TAA "IS-\\ T&.0 -uK-4 ^*n TM.-S 85.6% pH(S.U.) "f-C>>o 7.^ 7.7*r 7.75 -Z57 a<<sh DO (mg/L) &o

t

~~£* £i 7^5 e.i Conductivity (urahos/cm) /65~ i^;?:^:i W3 /<J0 ^Temperature (°C) TH-T 1S--L IS-D~~

~~y&.t-~~

~~-L4.i "IS-O 100% pH (S.U.) ^SS" 7-^3 7-73 7.7M 7.5-/ a.uft DO (mg/L) 8.Z Si £l i?H 7-i? ©.1 Conductivity (Mnihos/cm) ts? l;:f.:.-vS?:r;v^r /V? m Alkalinity (mgCaCOs/L) S<\\ m^~~

~~Hardncss (mgCaCOj/L) sA sg~~
~~TR chlorine (mg/L)~~
~~o.io~~

~~?* \\ A2'*'^j.'^'^i'V *Ti ^ojo 5^FJ-i?^; ?*'~~

~~Temperature (°C)~~

~~T£-D 14-<f 1S.D TH-/ "W.^~~

fl.*

~~100% Intake pH (S.U.) ^*57/ 7.7H "7-^6~~

~~7.70 7HH au^~~

~~DO (mg/L) S* &3 fi.'S 8.Z 7-tf 0,0 Conductivity (umhos/cm) tf? d^lSyj-'C^i: /^ ';**_*.^fe^-j /3^f ^Alkalinity (mgCaC03/L) Sto -.'.....*-VlA^i-. -SL.~~

Hartlncss (mgCaCOVL) s*

~~Sl-~~

~~TR chlorine (mg/L)~~

^O.lQ ^taLtofi sssmm ^O.'O ^^i^^ ^Temperature (°C) 1 TS.O TS.O TS-0 is.o 1+1 ^.H J Initial Final Initial Final Initial Final Temperaturesperformedat the time oftest initiation,renewal or temiinationby the analystidentified in the Daily Renewal Information tablelocatedon Page I. Alkalinity, hardnessand totalresidual chlorineperformedby die analyst identified on the bench sheetspecific for each analysis and transcribed to this bench sheet by: \\A SOP ATI 1-Exhibit ATI 1.2, revision 11-01-14 Page 47 of 91 n=3c?pi s. " J (mfaBfteMMJtTtitiftgScfcixtenLbe. Pa°e 7 of7 Species: Ceriodaphnia dubia Client: TVA / Seauovah Nuclear Plant. Outfall 101 Date: 10-09-18 Analyst Day (Analyst identified for each day, performed pH, D.O. andconductivity measurements only.) 3 4 5 6 YU esu TS TS MS A*F Concen tration Parameter " v#C ,^ -,'v.! -1.*" ~ >r ' CONTROL pH (S.U.) ^i T-.uo >.6o 7.6? 7.70 7-^^ "7.V9 7.6/ DO (mg/L) \\%

~~2,o~~

~~>X ?.o 7.7 ^B 7.7 ¥.? Conductivity (umhos/cm) 3oo &*#& l>'^*"* ^h?^^ 5/3 313 ,"; *C- '.."~~

~~Alknlinity (mgCaCOj/L)~~

^e 58 .*--.*..->-7:>>r.^ X.*

~~.^p-.'^f^.T V~~

"*£A7, **;-..-*

~~Hardness (mgCaCCb/L)~~

~~A\\ ^£&] &o '^x /JJS-^S?^ JSi^ ^Temperature (eC) -t*\\..-\\ -i&.\\ "^4 -l&JSLm.~~

~~H.t lAA "v<<\\A I.S.T-10.7%~~

~~pH(S.U.) 1^4 T-.S8 ~%.\\jifi 7.69 7.^0 7.V/ 7-so 7.51 DO (mg/L) fco 8.o ?.\\ 8./ ?.l 1.9 7.9 flo Conductivity (umhos/cm) a<^ Q1A~~

~~' '*--!-, 4 JL93~~

~~-**-r'^i.-Vv.*^ 2f& ^Temperature (°C) -e\\?<< W.fe -^*^ ~iM.f \\<<\\.<< 1H.<i "LH-i~~

~~v\\.t pH(S.U.)~~

~~\\-*5 "*,'5B) uz 7.68 7.77 l.HZ 7.V5 7.H8 DO (mg/L) 0.Q S-1 S.j <<-l ^?.l fto 79 S.\\ 21.4% Conductivity (umhos/cm) frTi .':* ;5,. 1-V <3u5 ^^i^i?! <?7? 2?7 ^Temperature (°C) -^rA -\\A.k~~

~~u.^~~

~~TS.V -vl. t -v&.\\ -li\\.£ xH-e 42.8% pH(S.U.) aiA T-.s-?- T-.Sl 7.$7 7.73 ^l 7.^5" 7MH DO (mg/L) 0.1 <&.o <&-t ^.l~~

~~9. a~~

~~(^O 7.^~~

\\

~~Conductivity (umhos/cm) olHO~~

', j'.^-;.,"^! 23*

~~^/5 ZHZ~~

~~Tcmperature (°C)~~

~~-\\A.$ -\\S.O ^."l Tj\\A~~

~~vA-8~~

~~-tH.f SiK't -iM-1 85.6% pH(S.U.) O.t/>>o T-.S5~~

~~^-.Sll.~~

~~7.64 766 7.Yo 7.29 7MO DO(rag/L) &.I ^ <&.l ?.l 5?p <<?l <<i7 &\\ Conductivity (umhos/cm) rr-b r--v~~

~~" vi:~~

M 4..S. >>jr*^>> >> 111 ".',^.>?- - sv /?/ Temperature (°C) -^.f- -iA.fr \\<<\\A "Vj4.^ lA-f 1S.0 "U\\-S -is.l 100% pH(S.U.) ^.*51^ -i.fia >.4<* 7.Co£l -7.te?> 7.35 7-36 7-35 DO (mg/L) 6.2 ?,$ <ai ^-a ^.3

.i
~~A 8.-L Conductivity (umhos/cm) 1*45 s~~

~~IM m ^v^iV<<^J-Tr. &H \\ '*?;" * \\. \\* ^Alkalinity (mgCaCOa/L) ,/'*>*"* st~ i*v ":'X*:~~

I
~~iJ "*-~~

~~-' /.vj."'^ ^j>>:,"l" . * ^.j %^-*' '~~

~~Hardness (mgCaCO-j/L) 14to'<<~~

~~^ _/ 4*.,/f^>>§. 'p.'.-.- ;*- - " j " ?.??(.';'\\'F~~

~~_,'.>. J t^*ivifj.r~~
~~TR chlorine (mg/L)~~

~~'v.* "'w* >>* ****. fir-r Y^'/JS <0.l0 .'^"'V^vV V~~

~~.'J.Sl" "i S!'JSS£l: L^3r27'5*Vi~~
~~Temperature (°C)~~

~~~\\s>>.o M-S TS.O~~

~~bl^~~

-lAA "IS.D TH-n XH.1 100% Intake pH(S.U.) %<5V "kS/ 7/+2 -7.&-? 7.&A ^.39 7^?& 7M DO (mg/L) d/ 9.>> Tjr.^ <<.a <<r ff.i &Z. Conductivity (umhos/cm) m^ \\3\\* "_t("^vr^:;.j /^7 /*// V^v} _.**';

~~AlknIinity (mgCaCOs/L) l.r^M;4 t wisJuS-*<<\\ - 'i*J>>i St-6}"~. ^x~~

~~~< B7'.,.'.7.., ^Hardness (mgCaCOj/L)~~

\\

~~l^fi"^^ -~-^-.~-^~~

~~TR chlorine (mg/L) rJV - -; ^T^-V~~

~~-It <0*l0 ~:'".J'r~5M'"AS5~~

~~7 t\\~~~~

~~"~ tV( *r -J*^^^*'- -i-e "*^f* ^ ~*) "-.?_ '.**--C^ -^ i." *,c ^Temperature (°C) T4.<\\ VM i>\\.e 14A m..n iH.ff Vl.H TS\\ Initial Final Initial Final Initial Final Initial Final~~

Temperatures performed at die lime oftest initiation, renewal ortennination by the analystidentifiedin the DailyRenewal Infonnationtable locatedon Page L Alkalinity, hardnessand totalresidual chlorine perfonned by the analyst identified on the bench sheetspecific foreach analysis and transcribedto this bench sheet by:

Page 48 of 91 SOP ATI l -Exhibit ATI 1.2, revision II-0M4 s <Q CD O \\ Environmental Testing SoluUons, Inc. TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated October 09-16,2018 Ceriodaphniadubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-0I3, Method 1002.0 Daily Chemical Analyses Project number: 13633 Concentration Parameter Day 0 Dav 1 Dav 2 Day 3 Day 4 Day5 Day 6 initial Final Initial Pruul Initial Final Initial Final Initial Final Initial Final Initial Final Control pll(SU) 7.09 7.82 7.75 7.80 7,59 7.58 761 7.60 7,50 7.69 7.70 7.40 7.49 7.61 OO (niR/L) 77 7.9 7.8 7.9 7.9 7.9 7.7 8.0 7.8 8.0 7,7 7,8 7.7 7.9 Conductivity (umhos/cm) 319 316 302 300 297 313 313 Alkalinity (mjj/L CaCO^) 60 59 58 Hardness (nig/L CaCOj) 84 80 8(1 Temperature fQ 24.7 25.1 24.8 25.1 24.7 24.8 24.7 25.1 24.8 25.0 24.8 24.9 24.7 25,2 10.7% pH (SU) 7.76 7.81 7.S6 7.80 7.78 7.58 7.74 7.5S 7.66 7.69 7.80 741 7.50 7.51 DO (me/L) 7.9 8.0 7.8 7.9 7.7 8,0 8.0 8.0 8.1 S.l 8.1 7.9 7.9 SO Conductivity (umhos/cm) 280 308 279 296 282 293 296 Temperature ("C) 24,8 25 1 24.9 25.0 24.7 24 s8 24.8 24.8 24.9 24.8 24.8 24 9 24.8 24.8 21.4% pM (SU) 7.73 7.80 7.83 7,79 7.75 7.57 7.75 7,59 7,62 7.68 7.77 7.42 7.48 7,48 DO (me/D 7.9 8,1 7.9 7.9 7.7 7.9 8,0 SI 8.1 8.1 8.1 8.0 7.9 8.1 Conductivilv (jimhos/cm) 264 285 262 272 265 279 277 Temperature (°C) 24.S 24 9 24.9 24.8 24.7 24.8 24.8 24.8 24.9 25.1 24.8 25.1 24,8 24.8 42.8% 1)11 (SU) 7.69 779 7.81 7.79 7.69 7.56 7.67 7.57 7.59 7.67 7.73 7.41 7.45 7.44 DO (me/L) 7.9 8.1 7.9 8.0 77 8.0 8.1 8.0 8.1 8.1 8.2 8.0 7.9 8.1 Conductivity (umhos/cm) 234 249 232 240 232 245 242 Temperature ("C) 24.9 25.1 25.0 24.8 24.7 24.8 24.8 25.0 24.9 24 9 24.8 24.S 24.8 24.7 85.6% pll (Stl) 7.60 7.74 7.75 7 75 7.57 7,50 7.60 755 752 7.64 7.66 7.40 7.39 7.40 DO (mg/L) 8.0 S.l 8.0 8.1 7.8 S.l 8.1 79 SJ 8.1 8.2 8.1 8.0 8.1 Conductivity (pmhos/cin) 165 173 160 173 169 177 171 Temperature CO 24.9 25.2 25.0 25.2 24,8 25.0 24,8 24.8 24.9 24.9 24.8 25.0 24.8 25.1 100% pll (SU) 7,55 7.73 7.73 7.74 7.51 7.48 7 56 7.52 7.46 7.62 7.63 7.38 7.36 7.35. DO(ntR/L) 82 8 1 8.1 8, J 7.8 8.1 8.2 7.8 8.1 8,2 8,2 8.1 8.1 8.2 Conductivity (umhos/cm) 139 147 134 145 139 148 144 Alkalinity (mg/L CnCOj) 54 50 52 Hardness (mg/L CaCOj) 54 50 54 Total Residual Chlorine (mg/L) <0I0 <0.I0 <0 10 Temperature ("C") 25.0 24.8 25.0 24.8 24,8 24.8 25.0 24.8 25.0 24.9 24.9 25.0 24.7 24.9 100% Intake nH(SU) 7.51 7.74 7 76 7.70 7.44 7.42 756 7.51 7.48 7.62 7.62 7.39 7.36 7.32 DO(mR/L) 8.2 8.3 8,3 8.2 7.8 8.0 8 1 7.8 8.1 8.2 8.2 8.1 8.1 8.2 Conductivity (umhos/cm) 137 148 134 142 136 147 141 Alkftiinllytiufi/LCaCOj) 56 52 52 Hardness (mg/L CaCOj) 58 52 54 Total Residual Chlorine (me/L)

~~'O.m~~

~~<().I0 <0.10 Temperature (*Q 250 25,0 25,0 25.0 24,9 24.9 24.9 24.9 24.8 24.9 24,7 24.8 249 25 1 File sqnl01 _ 100918chem Entered by T Sleeper Reviewed by~~

~~rf^?33pg$^~~

I?>>v>>roft<<<<f>>firit<<ttrtgSolu^onk tne. Page //? Page_/ of l_ Total Residual Chlorine (4500-C! 6-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL =0.10 mg/L DPD: Analyst Date analyzed J&. l(\\>(ft>\\% Positive and Negative Control: Control Type Sample ID Result K) Positive Negative Negative control Deionized water ^ Positive control Tap water V CHM IQ\\~l Sampie screening: Sample number Sample ID Sample characteristics Result (V) Positive l Negative l&lOOl.CTL-T\\/PA<^3 lO\\ f/QM" ytlloiAtckur v/ \\&ioo4.Cb 4/ i*Wfct£ //<jh+ yclloio.c/earj paHjeles \\S i£>>oo£.0 \\ B^\\tf2e%oS> //gM +00, ckar, {taftlctcS f ifiDCW.Q^A "fy^U u>njT* (* no cofar, clear V l^/DO^-tS R\\e>^\\uX no ctbf, clear iS/DO^-ft^ Msk\\JLX //5M -ten. clear w" isidcA-gr-Cft<KFrf^O0#\\ ye//ou>, e'eft/-, particles \\S ItiOO^O^ Li\\HfUfrtVpe^- //gh+ +Qf\\ clear V* 1*100*.oV p<<VWAl^ ^ght -rao, c/car;parhdcS \\s* ^ ^^^ ^^ ^^ io-*> <t^ ^^" ^^ ^1 1 Note: All sampleswere analyzedinexcessof EPA recommended holding time (15minutes)unlessotherwise noted. ApositiveresultIndicatesthe presenceoftotal residual chlorine abovedetection{> 0.10mg/L), which resultsina pink to red colorchangewiththe addition ofthe DPD indicator. A negative resultindicates the absenceoftotalresidual chlorine, which results innocolorchange withtheaddition of the DPD indicator. Page 50 of 91 Reviewed by Date reviewed n JQ-64-if SOP C8-Revision 4-Exhibit C8.1 Page 5H Page J of l_ Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L, Meter: Accumet Model AB250 pH/mV/lon Meter Iodide reagent: Acid reagent: T5 'MR %Q /O'09-l% 'NR %2>5 Analyst Date analyzed Caiibration: 0.10 mg/L 1.00 mg/L Slope (mV) (suggested range =26 to 30 mV) Reference standard # INSS /6/7 INSS /#/7

?

Note: For samples with a residual chlorine of >1.0 mg/L, the samples must be diluted to be within the calibration range. Laboratory controi sample: Reference standard number True value (TV) (mg/L) Measured value (MV) (mg/t) %RS = MV/TVxl00 (acceptable range =90 to 110%) INSS /(op 0.50 0.552 /66M% Duplicate sample precision: Sample number Sample ID Total residual chlorine (mg/L) %RPD ={(S - D)/[(S+D)/2]}x 100 (acceptable range =+/- 10%) \\k Ibfcfi.Cft-WJsfttaifcY 5 6.00% I Duplicate D O.0O& -ts /a.o9./e_ Sample measurements Sample number Sample ID Total residual chlorine (mg/L) TV <0.05 mg/L Method Blank (MB)

~~0. 0/0 TV =030 mg/L Method Detection Limit spike sample (MDU)~~

O&Ll \\*iO0<i. £h -TMK\\<£}*i> 1^\\1KV& 60.003 /^ / ^ ^ / S" ^-^ ^-" 1o ^"^ r0^ w* -f1^ ^^ ^^ <<^r Note: All samples were analyzed in excess of EPArecommended holding time (15 minutes) unless otherwise noted. Laboratory control sample: Reference standard number True value (TV) (mg/L) Measured value (MV) (mg/L) %RS = MV/TVX100 (acceptable range=90 to 110%) INss /&j7 0.50 C.57 7 /03.H% Reviewed by Datereviewed l /fl-O^-if Page 51 of 91 SOP C8-Revision 4-Exhibit C3.2 EftvifBfua*aufTejtt<<9Sdutbott, tec Page_£M_ Page V of I Total Residual Chlorine (4500-CI G-2011),Screening Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L Analyst Date analyzed +/- JQ-HM& Positive and Negative Control: Control Type Sample ID Result K) Positive Negative Negative control Deionized water

/

Positive control Tap water w" DPD: CHM ion Sample screening: Sample number Sample ID Sample characteristics ResultK) Positive Negative \\<£>>o!D-^>\\ ^/\\\\<>0^\\M PfrLfi. Tf\\v\\ Cv&Ne.. fottvCAjeS <*/ tfrlOiO.YL-* \\j i^TftvCe^ \\ \\S > A / / / / / / / / > / / / / / y C-Yttl Note: All samples were analyzed in excess of EPA recommended holdingtime (15minutes) unless otherwise noted. Apositive result indicatesthe presence of total residual chlorine above detection (>0.10 mg/L),which results in a pink to red color change with the addition of the DPD indicator. A negative result indicates the absence of total residual chlorine, which results in no color change with the addition of the DPDindicator. Page 52 of 91 Reviewed by Date reviewed TL lo-io-i8 SOP C8-Revision 4-Exhibit C8.1

~~/*-*-~~

I

~~J Page. SB Page~~

/ of f_ Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L, Meter: Accumet Model AB250 pH/mV/lon Meter Iodide reagent: Acid reagent Analyst Date analyzed Calibration: tmjfe INR m 0.10 mg/L 1.00 mg/L Slope (mV) (suggested r.inge - 26 to 30 mV) Reference standard t) INSS /£>/? INSS It, '3

?&

Note: for samples *iM\\a residual chlorine of > 1.0 mg/L, the sample?: most be diluted to be within the calibration rang??. Laboratory control sample: Reference standard number True value (TV) (mS/L) Measured value (MV) [mg/L] %RS = MV/TVxlOO l;n-7eptarjie range = 90 to 510%) INSS /(,/*£ 0.7.0 0.5"3C/ Afl£. tf% Duplicate sample precision: Sample number Sample 10 Total residua! chlorine (mg/L) V'.'KPO = {(S - D)/[(S+DJ/2J] x 100 (acceptable range = i 1074) itlOIO. 1 t

~~TM*\\lfC ftftl S~~

lu.°ZJ Duplicate D £&.o\\6 /-</' /pyJ/ £3 Sample measurements Sample number Sample ID Total residual chlorine (mg/L) TV < 0.05 mg/L Method Blank (MB) ZjO-o&? TV = 0.30 mg/L Method Detection Limit spike sample (MDLs) 0.3Z2 ) i IDlO.7.4 T\\l

~~ucjt u^e.L t~~

£ £->. £"-^/ l£ibl&.%\\ TsJ/\\\\ Saw mv Z<0-<<>>7 liioiO.SX-2L lUrwtt lO.O£>3 / Y / / 1 / ' r / f\\ An / r? ~~/ / A I/O/J? / / ° / <<" >'CJ / / ~y~ j£*__ Note.- Allsamples were analyzed it? excess of FPA recommended holding time (15 minutes) unless otherwise note Laboratory control sample: Reference standard number True value (TV) (mg/L) Measured value (MV) tmgAJ % RS - MV / TV X100 (acceptable range = 90 to 110%) INSS )ty-$ 0.50 O. <<>2 Joo-^^h Reviewed by

8*

~~Date reviewed /0-10-lf Page 53 of 91 SOP CS-Revision 4Exhibit CS.2 J fovfeenmsstitVotingSetntfitafc'"'* Page 11*5 Page \\~~

~~of_J, Total Residual Chlorine (450Q-CI G-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L Analyst Date analyzed ZS_~~

io-yb->fc Positive and Negative Control: Control Type Sample ID Result K) Positive Negative Negative control Deionized water sT Positive control Tap water DPD: CUM i0v^ Sample screening: Sample number Sample ID Sample characteristics Result (V) Positive Negative \\<£icm..ib TVKv^aw \\o\\ //<jM yellow, c\\<uxr) Y^hrJCTi l/ Uit>\\-i-x4

~~I lt*\\7\\K-cL~~

//ah+ yc//c^\\ ctor\\ car-ficlcS tX* MwaWllL-AUbAe*a<> /r^h-r-yt.tt<ju>, clear, r^ar-bclcs ^ UtttS. csh> "bONN> docOtP /ffjhV-tan, clear" IiiO^.0-\\ &*&tviiuLcL 00 Co/or, clear iito\\.-b - eS r\\r\\^ve-r //ghr vc/,W>, c/ei..' S i<iiDv^>.cru CAPST"P*i OO&A \\ld/ot>>,^/'5h^ c/cyci./( ()arHdcs V jAH p it r-UTfetCa =i /£ i Oi i>.D\\ B^THPfci /*ghV yci/Ok> t cVcar s ^J.**"- ^^ ^-^ ^__^ \\V

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// y ^y^ Wote: Allsamples were analysed in excess of EPA recommended holding time (15 minutes! unless otherwise noted. A positive result indicates the presence of total residual chlorine above detection (>0.10 mg/L),whichresults in a pinkto red color change with the addition of the DPD indicator, a negative result indicates the absence of total residual chlorine, which results in no color change with the addition of the DPD indicator. Page54of91 Reviewed by Date reviewed ¥ iSkJJzliL SOP C8-Revision 4-Exhibit C8.1 Page S U Page l_of !L Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L, Meter: Accumet Model AB250pH/mV/lon Meter Iodide reagent: Acid reagent: Analyst Date analyzed Calibration: TS 10 */&*/? INR %0 INR $35 0.10 mg/L 1.00 mg/L Slope (mV) (suggested range =26 to 30 mV) Reference standard #

~~nss /6 / 7 INSS /#/7~~

<<?? Note: Forsamples with a residual chlorine of > 1.0 mg/L, the samples must be diluted to be within the calibration range. Laboratory control sample: Reference standard number True value (TV) (mg/L) Measured value (MV) (mg/L) %RS = MV/TVxl00 (acceptable range =90 to 110%) INSS /(fill 0.50 0557 /Q*1*A Duplicate sample precision: Sample number Sample ID Total residual chlorine (mg/L) %RPD={(S - D)/[(S+DJ/2]} x 100 (acceptable range =+/- 10%) iitnllTl^> "TvIAvUg^ lOV. s 6.00% X 1Duplicate o 0.O07 im> JO'l&.HBr Sample measurements: Sample number Sample ID Total residual chiorfne (mg/L) TV <0.05 mg/L Method Blank (MB) Ln.ota TV =030 mg/L Method Detection Limit spike sample (lyiDLs) 0.313L IfclOVT-.lA 'Xvlft^Gv^ tw>rMft-t-O.OOA .....^^r....l ^ ^ ^ ^^ ^y^ 10" ^ ,V^ \\^>- 'OS ^ y ^^ Note: Allsamples were analyzed in excess of EPArecommended holding time (15 minutes) unless otherwise noted. Laboratory control sample: Reference standard number True value (TV) (mg/L) Measured value (MV) (mg/L) %RS = MV/TVxl00 (acceptable range =90 to 110%) INSS /£/7 0.50 0.537 105A V* Reviewed by Page 55 of 91 4t Date reviewed l /0 ^H<f SOP C8-Revision 4HExhibit C8.2 ^ Analyst Date analyzed vO 1P.0*H<<6

Page, Page *<*$*

j of J_ Alkalinity (SM 2320 B-2011) Matrix: Water, RL =5.0 mg CaCOa/L, Samples are titrated to pH =4.5 S.U. Titrant normality and multiplier determination: Titrant reference number INR &£{0 Normality check standard number 'nss y${ Begin mL 0*t> End mL 12.4 Total mL Normality (N) of HzSOa =(5mLNaaCO* x0.05J/E =0.25/E (acceptablerangeg 0.0180- 0.0220) 0,0202-pH Factor or Multiplier =(rV x 50000)/100 mLsample

~~Vx500 ia/~~

£4 Laboratory controlstandard (LCS): Reference standard number True value (TV) (mg CaC03/L) Sample volume (mL) Begin mL End mL Total mL Multiplier Alkalinity (MV) (mg CaCOa/L) %RS=MV/TVxl00 (acceptable range =90 to 110%) INSS I to~bQ 100 100 I2.H 7Z.Q °\\^ 104 <**M Duplicate sample precision: Sample number Sample ID Sample volume (ml) Begin mL End mt Total mL Multiplier Alkalinity (mg CaCOj/L) %RPD

~~{(S-D)/<<S+D)/2]}xlO0 (acceptable range =+/- 10%)~~

id.o&& Ssuu Joo &(<\\ %s*a 3.0 tai s 3o \\ Duplicate (D) J faO se\\ 3,l 4 D 31 z.zt Sample measurements: Sample number Sample ID Sample volume (mL) Begin mL End mL Total mL Multiplier Alkalinity (mg CaC03/L) MB (TV<2.5 mg/L) Deionized water. dH = 4."*> s.u. 100 0.O O.O O.O I0.I MO I O. D>>. 10 66wJ / Se.t 3I.I 3.0 I $n \\ D -02* +/-0 mitsuj Jl.S" 3>H s.^ vo O^atf.^fl- \\ 3^.vi ^32.

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&\\ 01-&IaJ©P>> 1 <B.£ <<4^Z {0.0 U/ UlooM' ol CjNtmh&dU tefl &>.V> k-%- J fr ^^ 1KS rfP J K ^ yS s' a./ ^ Laboratory control standard (LCS): Reference standard number True value (TV) (mgCaCOs/L) Sample volume (mL) Begin mL End mL Total mL Multiplier Alkalinity (MV) (mgGaCOj/L) %R5 =MV/TVxl00 (acceptable range<< 90to 110%) INSS <<o3-6 100 100 <&*<0 3<aO <M l04 q^ asr.ov, Reviewed by: 5C Date reviewed: 1 /^yffl-i<fr Page 56 of 91 SOP C6-Revision 4-Exhiblt C6.1 i Analyst Date analyzed 8Ss J'S^iriiLS_ Page Page 1 of _g^ Alkalinity (SM 2320 B-2011) Matrix: Water, RL - 5.0 mg CaCCb/L, Samples are titrated to pH-4.5 S.U. Titrant normality and multiplier determination: inr3.ni reference number Normality check standard number Begfn mL End ml Total ml (E) Normality (N) of H;SO.-.

~~(5 mLNaiCOl x 0.05)/E~~
~~0.2.5/E (acceptable range << 0.0180 - 0.0220]~~

~~pH Factor or Multiplier~~

~~(Wx50000)/ too mL samph sNjfSOfl INR lNSS i^SA-C.t>~~

I2.D V2..0 0.O2-C2 -o4 Laboratory control standard (LCS): Reference standard number True value (TV) (mg CnCOj/l) Sample volume (mt) Begin nit. End mL Total mL Multiplier Alkalinity (MV) (mg CnC03/L) K RS s MV / TV v 100 (acceptable range - 90 to 1105-51 INSS ll/3<s 100 100 Ii.o <<?>>.H

~~.h io.*f s-s~~
~~J?.otoi>>~~

Duplicate sample precision: Sample number Sample ID Sample volume (mL) Befiin mL End ml Total nl Multiplier Alkalinity (mg CaCOi/JJ '.rpi > = {(S-Dl/((S<<-D)/2])xl00 (acceptable range = i 10! io>ue>.ie&

~~&IA <<?f.3 &A tort 3o I Duplicate- (D) J-~~

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)DO ei ^^iCL1 Laboratory control standard (LCSJ: H>>2. 101450 "f^kH-^mx^^tandard 1 Truevalue(TV) number P~TjnTr&>>{7<5;./U__ Sample __volume Begin mL End mL Total mL Multiplier Alkalinity (MV) (mg cacoj/L) % RS << MV / TV X100 (acceptable range - 90 to 1105i) (ml) INSS 100 100 1 E>3l tc.^.iS Reviewed by: A, Date reviewed: l lQ.m.L£> Page 57 of 91 SOPC6-Revisior,4-txhibi! C6 I Page Page 4-T A of 5> Analyst Date analyzed J&* IQ-^.ig Alkalinity (SM 2320 B-2011) Matrix: Watet, RL = 5.0 mp, CaC03/L, Samples are titrated to pH -- 4.5 S.U. Titrant normality and multiplier determination: TitrSnt refr:rr<<ir! number i N H Uoaiia!iUi_check standard number-INSS Uegin End Normality [rjj of rfeStfc <(5 mLNa?COj x 0.0S)/E = 0..75/E ~tatTOpteble-Kia6£-aiL018Q - 0.0220) pH Factor or Multiplier (W x SOO0O)/ 100 mLsample -<<x 500 Q&i io-v^.q Laboratory control standard (LCS;- Reference standard number True value (TV) [mg CaCO>/L) Sample volume (mL) Blgjn mL End mL Total 117L Multiplier Alknlinity (MV) (mg CaCOj/L) % ItS a MV / TV x 100 (acceptable rang?.- = 30 <o 13f:."t) mss &V3S 100 100 D.O cj.4 <<w I0.4- <*8 %.0°fo Duplicate sample precision: Sample number Sample to mple volume (ml.) Begin ml End Total mL Multiplier Alkalinity [tng taCOs/L) 55RPO =<<5-D)/((S+O)/2],xlO0 (acceptable range = +/- 105>>) i£ioo°\\.o\\ &ftbbna'~\\ I 26 <<*M a.M-Z.o (#v> 10.4 17<7 1. Duplicate (D) -i-QM-iS.<4 3.o t0 -i-D &$/_ -jo.ia-l't Samole measuremantb Sample number Sample IP MBIT .:..:.?;** Deiortized water, pi I >>^ lQ\\1. lTU- £ti$kmur\\ V£-iOi%.0\\ UHDCft.flt-dTetftenaayL, I U-iOU.cfb> l£ib>>*>. T t^tDio.n-& T//i tec oc>> -1Ajxuq_-^£l I WiXkL 15'O^arlJ? mtfsu? ttU JfllSTLdiL

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33.<< Total ml MuJUpHei Jfe< il^iiuo^L H.o tk> \\ Sm it) Jc4_ it). tal Alkalinity Imr. CaC03/L) io->3> -\\S tuo 13lQ_ 2liL£^ _<i3o_ .i2iKL &g ilo fi aySgflMlHgjlg loM j£3"^MM (t>cn3 Jl JLi. 5>-U laj. 54-At4jjvj J5o_ JLk_li4 54 U2l SM 5b 35_ jill 52 111 r Laboratory control standard (LCS): ^ftsrftrefvc-e^tandacd number True value (TV) (mgCStbi/'tT Sample trcHonK? (ml) Begin ml End mL Total ml Multiplier Alkalinity (MV) (mg CaCCh/L) % RS

~~MV / TV x 100 (acceptable range = 90 to 110%)~~

INSS 100 100 ~6Su. io.i^s Reviewed by: tA" Date revhewed: 1&4H.W Page 58 of 91 SOP CS-Revision ^-Exhibit Cb.J f*fPC Analyst Date analyzed e&u tcf&'rt 4fe Page faf^**. Page K of 3, Alkalinity (SM 2320 B-2011) Matrix: Water, RL -- 5.0 mg CaCOs/!., Samples are titrated to pH = 4.5 S.U. Titrant normality and muitipiier determination INF. Laboratory control standard fl.CS}: Reference standard

~~"Humbi?r True value (TV)~~

(mg CaCOiA) Sample volume (ml.) Begir mL End ml Total ml. Multiplier Alkalinity (MV) (mg CaCOs/L) %RS =MV/TVx 100 (acceptable range = 90 to 110?i) INSS loo""---. --.. -i *'"*** Duplicate sample precision: Sample number Sample ID Sample-volume (ml.) Begin mL end mL

~~Tcita4, mL Multiplier AtkaHnlty (mg CaCOi/L) 5JRPD~~

= {(S-O)/((S+D}/2j)xlQ0 (acceptable range i i 1054) s ^-^ ^__ Duplicate (D) n ~"~~-es~ C*lSnjJ Sample measurements: Sample number Sample ID Sample volume (mL) Begin mL End mL Total ml Multiplier Alkalinity (mg CaCOj/L) MC (TV i 2J;-mt7tf- -tJEionnexi'WilTfer, pH - su ! *10 66^- !c.-v5-,ft, ItlDC^.O^ "TUaSQcO IkJTOJ \\ 6b %.s IO.T. 3.T- (.z\\ 10.4 5w IfclBrfl.Vl~ 2. \\ let >>."? <<*.S LV) l tjHtkX'L.fi 3 J-is?,** iS.l X.<L w GSL -^*-^ J / i / s' / X /c " OS* i>>n£ttg Laboratory control standard (LCS}: Reference standard number True value (TV) (mg CaCOj/t.) Sample volume (mL) Bcgfn mL End ml Total ml Muitipiier Alkalinity (MV) (mg CaCO,/L) KRSsMV/TVkIOO (acceptable range = 90 to 1105iJ INSS 11/3^ 100 100 \\5.t #fc£ 1-3 10.4 opt

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~~Reviewed by: It Date reviewed: IJOiiirill^ Page 59 of 91 SOP C6-Revision 4Exhibit C6.1 I fe-Ikfe 21~~

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Analyst Date analyzed (^ ia Jfrfl Titrant normality and multiplier determination: Titrafit reference number INR 0^>>4 Normality check standard number INSS 112J& Begin mL J2JL End ml Total ml 10a) i o.o Page Hardness (SM2340 C-2011) Matrix: Water, RL=5.0 mg CaC03/L Normality (#) of EDTA <<0.2/E (acceptable range << 0,0180 - 0.0220) 0-0200 pH Factor or Multiplier i {# x 50000)/ 50 ml sample ZD'O Laboratory control standard (LCS): Reference standard number True value (TV) (mg CaCOs/L) Sample volume (ml) Begin ml End mt Total ml Multiplier Hardness (MV) (mg CaCO$/q %KS=MV/TV*!00 (acceptable range =90to 110%) INSS R-Ol 40 50 too l2-o l^O too Ho l 00.O7. Duplicate sampie precision: Sample number Sample 10 Sample volume Begin mL End ml Total ml Multiplier Hardness (mgCaCOs/l) %RPD =((S-D)/KS+D)/2J)K100 (acceptable range - 110%) a#-r\\>F*rt 0D 17.0 K.% 0.2> 70,0 S U-O Duplicate (D) 1 \\7j& \\ D UOCTfttf Sample measurements: Sample number Sample ID Sample volume (ml) Begin mL End ml Total mL Multiplier Hardness {me CaCQs/L) MB{TV<2.Smg/U Deionlzed water 50 0.& 0-o o.o 26X3 >>x<<0 COh MP op I (7.V0 \\T<e> 0.£- f 4.0 *^iO anerr &\\ t2ft >>3. I 0 3 (4.0 to^fer UP foi >>3ju1 <0^ U'^l Mt/>£}tf0 rcdt^ gb> ai lb Ol n./ 3.V SO 3-*ft "i^ai^M J Ofl ^O il>i n.s o,u 60 i£-/6o4.o\\ Oarnhtt-de

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Analyst Date analyzed Q&\\. lo-v3-i% Titrant normality and multiplier determination: Page. Hardness (SM 2340 C-2011) Matrix: Water, RL = 5.0 mg CaCOj/L Page 4Q 2* of_a_ TTfrsmrefaasnce number Normality check ' Standardjuimber_ Eegin mL End mL Total mL Normality (<<) of EDTA = 0.2/E pH Factor cr Multiplier = (N x SOCOO)/ SO mLsample =<<xl000 (ET~ (acceptable range * (JillUU-UJJ<<<<af~ iNR INSS

~~e<<5i-i^iS'xf, Laboratory control standard (LCS):~~

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~~Vo lOD.O°L' Duplicate sample precision:~~

Sample number Sample ID 1^4 Sample (ml) ite^in mL End ml Total mL Multiplier Hardness (mg CaCQj/l) 5SRPD = {(S-0)/([S+D)/2]}xlO£l (acceptable range = +/- 10 >] i £/nit>.VI-SGixHwr Z 50 5s.r act <a.u> 2-0.0 55. \\ Duplicate (D) X atf..i ^.7 =?.u- -X 0 3"?s PgL l>=-(5,.^' Sample measurements: Sample number Sample ID Sample voiume (mL) Begin mL End mL Total mL Multiplier Hardness (mg CaCOs/L) MB (TV c2.-7Jiiit,/L)

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Test date 7-day IC25 =25% inhibition concentration. An estimation ofthe potassium chloride concentration which would cause a 25% reduction in Pimephales growth (calculated using ToxCalc). Central Tendency (meanlogarithmic IC25 converted to anti-logarithmic values) Control Limits (meanlogarithmic IC2S +/-2 standarddeviations converted to anti-logarithmic values) Laboratory Warning Limits (meanlogarithmic IC,5 +/-2 coefficent ofvariations convertedto anti-logarithmic values) USEPA Warning Limits (mean logarithmic IC25 +SA 75 convertedto anti-logarithmic values, SA 75 =75lh percentile ofCVs reported nationally by USEPA) Mm Sumnet Page 65 of 91 Pimephales promelas Chronic Reference Toxicant Control Chart Source: In-house Culture TO tnVJronnwital Testing Srtlutlam, Int. o ^§t number Test date 7-day IC25 LogjaConversion AntMogarithmic Values (g/L KCf) Laboratory Calculated CV 75th Percentile CV ToxCal Determination 7-day ICM a S CT Control Limits Warning Limits Warning Limits (g/L KCl) CT-2S CT + 2S CT-2CV CT + 2CV CT-SA,75 CT+SA.75 1 05-02-17 0.6740 -0.1714 -0.1371 0.0316 0.7292 0.6305 0.8434 0,5939 0.8858 0.4521 1.0063 2 05-16-17 0.6906 -0.1608 -0.1382 0.0320 0.7275 0.6278 0.8431 0.5904 0.8864 0.4511 1,0040 3 06-06-17 0.7367 -0,1327 -0.1393 0.0314 0.7256 0.6280 0.8384 0.5911 0.8810 0.4499 1.0013 4 07-11-17 0.7519 -0.1238 -0.1393 0.0314 07257 0.6280 0.8385 0.5911 0.8811 0.4499 1.0014 5 08-08-17 0.6829 -0.1656 -0.1379 0.0294 0.7280 0.6357 0.8336 0.6012 0.8731 0.4513 1.0046 6 09-12-17 0.7271 -0.1384 -0.1371 0.0292 0.7294 0.6377 0.8342 0.6037 0.8730 0.4522 1.0065 7 10-03-17 0.7024 -0.1534 -0.1382 0.0293 0.7274 0.6355 0,8326 0.6011 0.8720 0.4510 1.0038 S 11-07-17 0.6666 -0.1761 -0.1421 0.0289 0.7210 0.6310 0.8238 0.5961 0.8636 0.4470 0.9949 9 11-07-17 0.6847 -0.1645 -0.1404 0.0265 0.7237 0.6406 0.8175 0,6089 0.8533 0.4487 0.9987 10 12-05-17 0.7191 -0.1432 -0.1424 0.0251 0.7205 0.6418 0.8089 0.6112 0.8432 0.4467 0.9943 11 01-09-18 0.7574 -0.1207 -0.1397 0.0244 0.7249 0.6478 0.8113 0.6185 0.8441 0.4495 1,0004 12 02-06-18 0.7951 -0.0996 -0.1377 0.0260 0.7283 0.6461 0.8211 0.6154 0.8557 0,4516 1.0051 13 03-06-18 0.7002 -0.1548 -0.1387 0.0263 0.7266 0.6437 0.8201 0.6126 0.8552 0.4505 1.0027 14 04-03-18 0.6973 -0.1566 -0.1411 0,0256 0.7225 0.6423 0.8128 0.6115 0.8474 0.4480 0.9971 15 05-08-18 0.7204 -0.1424 -0.1429 0.0243 0.7196 0.6435 0.8048 0.6138 0.8380 0.4462 0.9931 16 06-05-18 0.7512 -0.1243 -0.1451 0.0200 07160 0.6530 0.7852 0.6280 0.8126 0,4440 0.9881 17 07-10-18 0.7808 -0.1075 -0.1429 0.0216 0.7196 0.6514 07950 0.6248 0.8244 0.4462 0.9931 18 08-07-18 0.7690 -0.1141 0.1410 0.0225 07228 0.6518 0.8015 0.6245 0.8317 0.4481 0.9974 19 09-11-18 0.6884 -0.1621 -0.1432 0.0222 07191 0.6491 07967 0.6217 0.8270 0.4458 0,9924 20 10-09-18 0.6735 -0.1717 -0.1442 0.0231 07175 0.6452 07979 0.6167 0.8296 0.4448 0.9901 Note: 7-day IC2S =25% Inhibition concentration. An estimation ofthe potassium chloride concentration that would cause a25% reduaion in Pimephales growth (calculated using ToxCalc). CT>> central tendency of the IC3S values. S = standard deviation of the iCj5values. Control Limits =Mean logarithmic IC +/-2standard deviations converted to anti-logarithmic values. Warning Limits

~~Meanlogarithmic IC2S t 2CV orsA.<<, convertedto anti-logarithmic values.~~

SA.,S ~Standard deviation corresponding tothe75"' percentileofCVsteported natiorraliy by USEPA (5A75 *0.38). CV = Coefficient or variation. r4/rt>t\\trtv4 m*a f = tmlnwuwwiut T.tttng Sekritoni, tec Pimephales promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture Q) JC CO

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&<$> Ktf8>,*& kk&-*& Test date Control Growth, Coefficient of Variation (CV)or Percent Minimum Significant Difference (PMSD) PMSD is the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is not a minimum acceptance criteria. Central Tendency (mean Control Growth, CVor PMSD) 95% Confidence Interval (mean Control Growth, CV or PMSD +/- 2 Standard Deviations) Page 67 of 91 £nip<<*dand Reviewed by Urn Sumner litttormuMtHmtrq tgtatMm,t>> Pimephales promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture s-4 Test number Test date ToxCal Determination Control Growth (mg/lnltiai larvae) Control Growth CV Test PMSD (%) Control Survival Control Growth Test Mean CV MSD PMSD a 95% Confidence Interval CT 95% Confidence interval a 95% Confidence interval (%) (mg/initial larvae) i<<) (%) CT-2S CT + 2S CT-25 CT+2S CT-2S CT + 2S 1 05-02-17 100 0.729 5.6 0.0792 10.9 0.596 0.416 0.776 6.2 0,6 11.8 11.4 5.0 17.8 2 05-16-17 100 0.593 2.8 0.0521 8.8 0.603 0.434 0.771 6.0 0,2 11.8 11.4 5.0 17.8 3 06-06-17 100 0.608 7.2 0.0542 8.9 0.601 0,434 0.768 5.8 07 10.8 11.0 5.2 16.8 4 07*11-17 100 0.713 3.3 0,0589 8.3 0.609 0.438 0.781 5.6 0.5 10.7 10.8 4S 16.8 S 08-08-17 100 0.638 7.9 0.0791 12.4 0.616 0.451 0.781 5.9 1.0 10.8 11.0 5.1 16.9 6 09-12-17 100 0.631 5.6 0.0562 8.9 0,626 0.489 0.764 5.9 1,0 10.8 10.8 5.0 16.5 7 10-03-17 100 0.658 4.4 0,0838 12.7 0.637 0.525 0J48 6.0 1.2 10.7 11.0 5.4 16.6 8 11-07-17 100 0.738 3.9 0.0641 8.7 0.645 0.528 0.761 5-7 1.1 10.4 10.8 5.2 16.4 9 11-07-17 100 0.727 0.8 0.0580 8.0 0.642 0.535 0.749 5.3 0.6 10.0 10.5 5.0 16.1 10 12-05-17 100 0.676 2.3 0.0607 9.0 0.647 0.545 0,750 5.2 0,4 10,0 10.6 5.0 16.1 11 01-09-18 100 0,612 8.3 0.0752 12.3 0.643 0.543 0.744 5.4 0.5 10.4 10.8 5.0 16.6 12 02-06-18 100 0.772 8.4 0.081S 10.6 0.651 0.538 0 765 5.6 0.5 10.7 10.6 5.0 16.2 13 03-06-18 100 0.810 4.7 0,0640 7.9 0.660 0.526 0.794 5.6 0.5 10.7 10.6 4.9 16.3 14 04-03-18 100 0.743 6.1 0.0836 11.3 0.668 0.534 0.802 5.8 0.8 10.7 10.7 5.1 16.3 15 05-08-18 100 0.576 4.9 0.0516 9.0 0.663 0.523 0.803 5.6 0.7 10.5 10.8 S3 16.3 16 06-05-18 100 0.643 8.7 0.0992 15.4 0.664 0.525 0.803 5.8 0.7 10.9 11.2 4.8 17.6 17 07-10-18 100 0.678 3.9 0.0850 12.5 0.669 0.535 0.803 5.6 0.5 10.7 11.3 4.9 17.7 18 08-07-18 100 0.692 6.4 0.0813 11.7 0.675 0.547 0.802 53 1.0 9.7 10.9 6.0 15.8 19 09-11-18 100 0.709 8.4 0.0680 9.6 0.678 0.551 0.805 5.5. 0.9 10.0 10.9 6.0 15.8 20 10-09-18 100 0.973 6.8 0.0693 7.1 0.696 0.516 0.876 5.5 i.0 10.1 10.6 5.5 15.7 Note: Control Survival = USEPAminimum test acceptability criteria 2 80% survtvai Control Mean Growth s USEPA minimum test acceptability criteria ^ 0.25 mg/su>>viving larvae. CV* Coefficient of variation for control growth. USEPA maximum CV guidance criteria (90th percentile) <20% MSD= Minimum significant difference. PMSDs Percent minimum significant difference. PMSD isa measure oftest precision. The PMSD istheminimum percentdifference between thecontrol andtreatment thatcanbedeclared statistically significant Inawholeeffluent toxicity test Lower PMSD bound determined byUSEPA (10,h percentile) >12%. Thelower PMSD bound represents a practicallimitto the sensitivityof the test method and isnot a minimumacceptance criteria. Upper PMSD bound acceptance criteria determined byUSEPA (90,H percentile) <30%. CT* Central tendency.of the growth, CVor PMS0values. S = Standard devotion of the growth, CVor PMSD values M ' Environmental TestingSolution!, inc. Potassium Chloride Chronic Reference Toxicant Test (EPA-822-R-02-013 Method 1000.0) Species: Pimephales promelas PpKCICR Test Number: 44 Page I of6 Dilution preparation information: Comments: KCl Stock INSS number: inss nv\\ Stock preparation: 50 g KCI/L: Dissolve 50 g KCl in i-L Milli-Q water. Dilution prep(mg/L) 300 450 600 750 900 1050 Stock volume (mL) 9 13.5 IS 22.5 27 31.5 Diluent volume (mL) 1491 1486.5 1482 1477.5 1473 1468.5 Total volume (mL) 1500 1500 1500 1500 1500 1500 Test organism information: Test information: Organism source: In-house culture Randomizing template: ^OlUuJ Age: < 24-hours old Incubator number and shelf location: n6 Spawn date: ua-tA-*fc Ariemia CHM number: CHM984 Hatch dates and times: to-o&-i£ l^x<* Drying information for weight determination: Transfer vessel infonnation: pH= -\\,SO S.U. Temperature<< ~V\\A °C Date / Time in oven: /D-ifc-tf tftMS Initial oven temperature: feO'C Average transfer volume: < 0.25 mL Date / Time out ofoven: ftm-tr crv4£> Final oven temperature: tVC Total drying time: -L+riouitb Dailyfeeding and renewal information: Day Date Morning feeding Afternoon feeding Time Analyst Time Analyst 10-09-18 OlO^ 4 ITOS S-10-10-18 OlQft \\XGfc 4-10-11-18 eho A-H.ID 4L 10-12-18 ftV>>lQ 4 n.ib -£* 10-13-18 CHbO +/- i^DO -&r 10-14-18 enco 4i-V^OQ -ft-10-15-18 fitoOO

f

~~\\2.\\o 10-16-18 Test initiation, renewal, or termination Time Analyst b&lU -Jr-O&lO Jt-olfu QglO 4 CAoO~~

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MHSW bntch used iO-dVI^ n-(sb-i<t /o-evtf u-<n-il ID-\\WfcA Ifr-iVifrft >il-if A. Control information: Acceplance criteria Summary oftest endpoinis: % Mortality: C>7. <20% 7-day LCso <§2G. NOEC 4SO Average weight per surviving larvae: OA-Xb > 0.25 mg/Iarvae LOEC <o0G ChV Sl<<.(* ICis ^VS Page 69 of 91 SOP AT21 - Exhibit AT21.1, revision I l-Ol-M ^stm Page 2 of6 EnvlronrnemalTeiWftjScttKion*,Inc. Species: Pimephales promelas PpKCICR Test Number: 44 Survival and Growth Data Day 1 Control 300 mg KC1/L 450 mg KCI/L A B c D E F G H I j K L 0 10 10 10 10 10 10 10 10 10 10 10 10 1 /O fO '0 1^ '0 10 >>o 10 10 10
0 10 2
/o /o. JO /o <<0 '0 '0 '0 >>0 <<0 <0 '0 3 '0 '0 10 ID 10 Ifc 10 <0 fO >D 10 '0 4 /o '0 '0 '0 10 10 /o 10 '0 to f 0 >0 5 /o (0 /o JO /o 'o CO '0 '0 /0 to 10 6 /O 10 /o /O 10 10 ib 10 /D t0 10 to 7 /O /o 10 /o 10 '0 '0 '0 'o /o <0 '0 A - Pan weight (mg) Traycolor code:: QCl^f Analyst: TS Date: Cl-A^ll? /3.W I4.<?a I3.<& ft# £07 13.40 M./o IW aai 13.?1 /3.#l 13/fc B = Pan + Larvae wcieht (mg) Analyst: \\A Tb.Qb Ti.l<? i>>,.it l^Sb w/u t&A* -a/tf *Ut-3b Tl.^ ivan rval TZ.&b Date: to-Wi£ C = Larvae weight (mg)=B - A IIn ltd calculated. Analyst: V\\ ^.0*8 iO.SU <\\A^ <\\.<<>> <\\.&4 V*8 1.a<\\ H-H-l <<V11 *S.su
16. IS
^.t.T Weight per initial number of larvae (mg) = Cl Initial number of larvae Hand calculated. Analyst: Y\\ ^ tf JSP 0" 0' A. 0' A. 0' Average weight per initial number of larvae (mg) Percent reduction from control (%) O.W? ,'^l,;;.'-: f--.,j.;.. o.^uS o.S.7. O.Vrt <>>7. Comment codes: c = clear, d = dead, fg = fungus, k - killed, m ~ missing, sk = sick, sm = unusually small, Ig = unusuallylarge, d&r = decanted and returned, w = wounded. Conmients: Page 70 of 91 SOP AT21 - Exliibit AT21.1, revision 11-0 i-14 Page 3 of6 EnvironmentalTestingSch>>tiw<<. Inc. Species: Pimephales promelas PpKCICR Test Number: 44 Survival and Growth Data Day 600 mj KCI/L 750 mg KCI/L 900 mg KCI/L M N O p Q R s T U V w X 0 10 10 10 10 10 10 10 10 10 10 10 10 1 to fO IO to '-0 fD to to g* s1* T* SM 2 tO >>0 IO 10 to to to to n S 3 (0 to 10 to ID IO fO <<Cs <£ £ n 8 4 'D
0 JO
<0 10 V* 10 V< -i* ^ i V41 5 10 <<0 V* S^ &l1 sM 1 sa 5* 3> s 4* 6 IO <\\ 10 °l S ^ "V* S
s q
7 ^*4. \\ <^<<\\ 1 Y* U> C n ^ "b 4"t 4 A = Pan weight (mg) Trav color code:: 3*^s/ Analyst: "TS v Date: Oq.2MAV IH.05 13.74 I3.3H Mb &W 0.31 D.M0 K?H £# mo M.tf £74 B = Pan + Larvae weight (mg) Analyst: Date: ll.T^ 2VUo
&.*& n.n 2T,tb zv.'to PU\\
"IV1& H.Sl U.M atf 20.40 C = Larvae weight (mg) =* B - A Hand calculated. Analyst: J[ £.>i S.Xl %M i.M C.T8 u.& s.H fc.V* 4.ofc. *2/W M.vl M.Ufe Weight per initial number oflarvae (mg) = C / Initial number of larvae Hand calculated. Analyst: V"\\ 0' NO o* ° 1 0" 0' 0' Average weight per initial number of larvae (mg) Percent reduction from control <%) 0.8^4 \\<,.V7. 0.W5/1-3S.l7. CVU s<*. \\ 7. Comment codes: c = clear, d = dead, fg = fungus, k - killed, m - missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Comments: Page 71 of 91 SOPAT21 - Exhibit AT21.I, revision il-Ol-14 "jGT* _. EnvJronmtoUJTciUngSc!o<<tent,Inc. Species: Pimephales promelas PpKCICR Test Number: 44 Survival and Growth Data Day 1050 m gKCl/L Y z AA CB 0 10 10 10 10 1 \\\\\\pA> s5* 5^ SsA 2 <A S S s 3 ^ S S s 4
b*
5 \\ 4 .4 6 x. \\ T_ T_ 7 z \\ l'* 1* A << Pan weight (mg) Tray color code:: cifeW Analyst: fS ° Date: OI'^'J* \\5.lb m.oo W.7I IH.57 B = Pan + Larvae weight (mg) Analyst: l( n.is vk>s\\ isnt js.v>>i Date: /0*n-t£ C = Larvae weight (mg)
B - A Hand calculated.
Analyst: V\\ iM o.&<<\\ t.oi LOS 0 ' Weight per initial number oflarvae (mg) -C/ Initial number of larvae Hand calculated. Analyst: jr\\ o" ^5 Average weight per inltiu] number of larvae (mg) Percent reduction from control (%) o.\\TS <si.n. Comment codes: c = clear, d = dead, fg - fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r - decanted and returned, w = wounded. Comments: Page 4 of6 Page 72 of 91 SOP AT21 -ExhibitAT21.1,revision H-Ol-14 F.miromncnUl TpMng Setuitoni, Inc. Pimephales promelas Chronic Reference Toxicant Test EPA*821-R-02-013, Method 1000,0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: Test dates: October 09-1$, 2018 CorwmlraiHiFt fmj/l KO) R<<(i1{faie inttbiflumtwof larvae final number of laivae A* Pm welfht (m<<) 0 a Pan t Urw>># Uivas weight Jwgl
0-A Weight / Survives number 61larvae (ml)
Mean unsigHf/ttmrivfot numbfff cH<<fv*e (mg} Gtxefffcient of variation <<vmt>>t4lwn<<<<J (iJJ Urvae lrnjj M<<*n turtfcal (H) M<<*Aw#lcht/Mllte> number at i>>rv>># fml> Cofiffttfent ot vaibiton m P<<rwnt reduction from contra! (Nl Control A 10 10 13.98 23.06 9.08 Q.90S 0.973 6.8 0.908 100.0 0,973 6.8 Not applicable B 10 10 12.62 23.13 10.56 1.056 1.056 C 10 10 13.22 33,16 934 0.994 0.994 D 10 10 15.23 24.56 9,33 0,933 0933 300 E 10 10 15.07 24.91 9,84 0984 0.965 3.3 0.984 100.0 0.965 3.3 0.8 F 10 10 13.40 23.38 9.98 0.S9S 0.998 6 10 10 14.10 23.39 9.29 0.929 0.929 H 10 10 14.89 24.36 9.47 0J47 0.947 450 J 10 10 13.21 22.98 9.77 0.977 0.979 2.6 0.977 100.0 0.979 2,6 -0.6 1 10 10 13,81 233? 9.56 0.956 0.956 K 10 10 1-3.24 2339 1045 1.015 1.015 I 10 10 13.15 22.83 9.67 0.967 0.967 600 M 10 9 14,05 22.79 8.74 0.971 0.915 43 0.874 90.0 0.824 4.3 153 N 10 9 13.74 21.96 8.22 0.913 0822 0 10 9 13.34 21.33 B.04 0.893 0.804 P 10 9 14.92 22,87 7.94 0.882 0.794 750 Q 10 7 15,88 22.66 6.78 0.969 0975 6.6 0.678 65.0 0.632 7.0 35.1 R 10 6 15.31 21.70 6.39 1J065 0.639 S 10 6 13.40 19.11 5.71 0,952 0.571 T 10 7 15.84 22.23 639 0.913 0.639 900 U 10 4 15.45 19.51 4.06 LOIS 1055 7.6 0.406 37.5 0.39S 18.5 59.1 V 10 3 13.40 16.34 2.94 0980 0 294 w 10 4 14.9S 19.19 4.24 1.060 0,424 X 10 4 15.74 20.40 4.6S 1465 0.466 10S0 Y 10 2 15.16 1745 i.98 0.995 1.001 8.1 0.199 12.5 0,125 40.0 87.1 z 10 1 14.00 14.89 0.39 0.890 0.089 AA 10 1 14.71 15.78 1.07 1.070 0.107 BB 10 1 14.57 15.62 1.05 1.050 0.105 Bunnell's MSDvalue: 0.0693 MSD* Minimum Significant Difference PMSD: _74 PM5D-PercentMinimum Significant Difference PM5Disa measure of test precision. ThePMSD ii the minimumpercent difference between the control and treatment that can bodeclared *jtatijticaliyvgmficant In9 Wtsole effluent toxicitytest. Lower PMSD bound determined by U5EPA(iQth percentile)
12%
UpperPMSD bound determined by USEPA (90th percentile)<< 30H. lower and uppar PMSD bounds vvare determined from the 10thand 90th percentile,respectively, of PMSD data fromIPA'sWET fmerlabcratory Variability Study{USEPA* 2001a; USEPA, 2001b). The lower PMSDbound represents a practical limit to the sensitivity of the test method and 12 not a minimum acceptance criteria Ig^o
"^*"J**>> ^ -c Statistical Analyses Ei^rotm>entarTfe£tl>>9 Sotutl<<ft>>, inc.
Start Date: 10/9/2018 End Date: 10/15/2018 Sample Date; Comments: Larval Fish Growth and Survival Test-7 Day Survival Test ID: PpKCICR Sample ID: Lab ID. ETS-Envir. Testing Sol. Sample Type: Protocol RA/CHR-EPA-821-R-02-013 Test Species: REF-Ref Toxicant KCL-Potassium chloride PP-Pimephales promelas Conc-mg/L D-Control 30Q 450 600 750 900 1050 1 1:0000 1.0000 1.0000 0.9000 0.7000 0.4000 0.2000 1 0000 1.0000 1.0000 0.9000 0.6000 0.3000 0.1000 1.0000 1 0000 1.0000 0,9000 0.6000 0,4000 0.1000 10000 1 000Q 1 0000 0.9000 0.7000 04000 0.1000 Conc-mg/L Mean N-Mean Mean Transform: Afcsin Square Root Mm Max CV% D-Control 1.0000 1.0000 300 1.0000 1.0000 450 1,0000 1,0000 1.4120 1.4120 1.4120 14120 1.4120 1.4120 1.2490
600 0.9000 0.8000 1.2490 T50 0.6500 0.6500 0.9386 0.8861

900 0,3750 0 3750 0.6584 0.5796

1050 0.1250 0 1250 0.3572 0.3218 1,4120 1.4120 1,4120 1.2490 0.9912 0.6847 0.4636 Auxiliary Tests Shapiro-Wilk's Test Indicates non-normal distribution (p -
Equality of variance cannot be confirmed =0.01) Hypothesis Test (1-tail, 0.OS) NOEC LOEC ChV Steers Many-One Rank Test Treatments vs D-Control 450 600 519.615 0.000 0,000 0 000 0000 6 464 7 979 19.861 Statistic 0.86556 TU Rank 1-Tailed Sum Critical 18.00 18.00 10.00 1000 1000 1000 10.00 10.00 10.00 10.00 10.00 10.00 Critical 0.896 Parameter Value SE Maximum Likelihood-Probit 95% Fiducial Limits Control Chi-Sq Critical P-value Mu Number Total Resp Number 0 40 0 40 0 40 4 40 14 40 25 40 35 40 Shew Kurt 0.55749 2.4279 Iter Slope intercept TSCR 10.0802 1.29616 7.53969 12,6206
24.376 3.77401
-31 773 -16.979 Sigma 0.54481 9.48773 0.96899 2.91423 0.0992 Point EC01 EC05 EC1Q EC15 EC20 EC25 EC40 EC50 EC60 EC75 ECHO EC85 EC90 EC95 EC99 Probits mg/L 95% Fiducial Limits 2.674 482,445 400.783 541.007 3.355 563.709 491,749 614 83 3.718 612.486 547.704 659.058 3.964 647.759 588.463 691.326 4.158 677.235 622.492 718.692 4.326 703.59 652.705 743.651 4.747 774.639 731.584 614.793 5.000 820.791 779.454 865.351 5.253 869.692 826.7 923.242 5.674 957.514 904.335 1036,48 5.842 994.776 935.374 1087.21 6 036 1040.04 972.077 1150.46 6.282 1099.94 1019.38 1236.43 6.645 1195.11 1092.42 1377.49 7,326 1396.42 1241.18 1690.55 Dose-Response Plot Page 74 of 91 100 1000 Dose mg/L 10000 £ Statistical Analyses
rrvrt'twTT>>f>>U)T<<i)f>5Soiu<*wi>>.tn<
Start Date. 10/9/2018 End Date: 10/16/2018 Sample Date: Comments: Larval Fish Growth and Survival Test-7 Day Growth Test ID-PpKCICR Sample ID. Lab ID: ETS-Envir. Testing Soi. Sample Type Protocol: FWCHR-EPA-821-R-02-013 Test Species: REF-Ref Toxicant KCL-Potassium chloride PP-Pimephales promelas Conc-mg/L D-Control 300 0.9080 0.9840 1.0550 0.9940 0.9330 0.9980 0.9290 0,9470 450 09770 0.9560 1.0150 0,9670 600 0.8740 0.8220 0.8040 0.7940 750 0.6780 0.6390 0 5710 0,6390 900 0.4060 0.2940 0.4240 0 4660 1050 0.1990 0.0890 0.1070 0 1050 Transform: Untransformed Conc-mg/L Mean N-Mean Mean Min Max CV% t-Stat 1-Tailed Critical MSD Isotonic Mean N-Mean 450 0.9788 600 D-Control 0.9728 1.0000 0 9728 0 9080 1,0560 300 0.9645 0.9915 0.9645 0.9290 0.9980 1,0062 0,9788 0.9560 1,0150 0.8235 0.8466 0.3235 0 7940 0 8740 0.631 B 0.6494 0,6318 0 5710 0.6780 5.807 3.316 2.620 4.324 7.040 750 800 0.3975 0.4086 0.3975 0.2940 0.4660 18.475 1050 0.1250 0V1285 0 1250 0.0890 0.1990 39.989 0.259 -0 189 2.180 2.180 0.0693 0.0693 0.9728 0.9716 0.9716 0.8235 0.6318 0.3975 0.1250 1 0000 0.9988 0.9988 0.8466 0.6494 0.4086 0 1285 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97485 BarttetTs Test indicates equal variances (p = 0.26) 2,69204 0.805 921035 0.45632 0.19391 Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob dt Dunnett's Test Treatments vs D-Control 450 >450 0.06931 0.07125 0.0002 0.00202 0.9047 2.9 Point mg/L SD Linear interpolation (200 Resamples) 95% CL<Exp) Skew IC05 498.11 64,08 68.37 523.00 -3.1814 IC10 547.37 22.37 449.63 595.31 -0.6658 IC15 596.62 21.74 503.54 637.47 -0.7512 IC20 635.44 17.65 561.63 667.88 -0.8234 IC25 673 48 16.69 607.02 708.82 -0.5824 IC40 780.80 14.84 720.53 813.59 -0.5453 IC50 843.09 14.63 793.67 881.03 -0.0458 Dose-Response Plot Page 75 of 91 500 1000 Dose mg/L 1-tail. 005 level of significance PEHE ' imimnmtM>>\\TetSagSaUnisni,lac. Species: Pimephales promelas Daily Chemistry: Page 5 of6 PpKCICR Test Number: 44 Day (Analyst identified foreachday,performed pH>> D.O.andconductivity measurements only.) Analyst 0 1 2 HT A*/
41 TS T<<,

0 Concentration Parameter
^V"!':^ IH^C":"^'1 ssijisi ESIffi? CONTROL pH(S.U.)
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^rw ^e 7.1 £0 Xio Conductivity (^mhos/cm) 3h Wi~^m 3>/C 3c& ^Alkalinity (mgCaCG3/L) uo V s<\\ ^Hardness (mgCaCOs/L) s4 ^
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-\\s\\-V>> -VJA-S -V\\A -wt.s TA-W -u\\.<i 600 mg KCI/L pH (S.U.) ^.£7 ^7*T ?.<?<Y "7"jS 79.2 an-DO (mg/L) 7.*? ^S SM 2.SL
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^0 Conductivity (umhos/cm) /37* '., --.*;. ^---r' 1310 yPi^%-£ /353 ^Temperature (°C) T.HA. -lAT -mKA "VA-lo 1>U -vA-i 750 rag KCI/L pH(S.U) "?-#* ^M ^TZ 759 7.91 'M DO (mg/L) S<< "*3- $L\\ 1.1 £.P ^.0 Conductivity (umhos/cm) /6>2o K-2S JC&O f&^-Xry. (5fO /-'*; ^fc;^ ^Temperature (°C) -fc\\-\\o T>\\-3 I'M -iA-2>> -V\\.\\o -bi-C 900 mg KCI/L pH (S.U.) "3.a& ?*<<? ^9Z -7.5% 1.90 ^rbo DO (mg/L) Si =?., $.2. fr.a £3 an, Conductivity {umhos/cm) /<?£%? , *l'*iC>> ' v'"' f -
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"HU IH-H ^4.1 TA.T TM.U -i+t Initial Final Initial Final Initial Final l
Teniperaluresperformed at the time of test initiation, renewalor terminationby the analyst identifiedin the Daily Renewal Informationtablelocatedon Page I.
Alkalinity and hardness performed bytheanalystidentified on the bench sheetspecific foreachanalysis and transcribed to tliisbenchsheetby:
\\A Page 76 of 91 SOP AT2 i - Exliibit AT21. i, revision 11-01-14
f pS3ET" Page 6 of6 ^i environmentalTiefllngSolutiam,lnc Species: Pimephales promelas PpKCICR Test Number: 44 Day (Analyst identified for each day, pcrfonncd pH, D.O. and conductivity measurements only.) Analyst 3 4 5 6 ^ <<S-ftS^ 75 T5 /vr rtP "7^ Concentration Parameter t\\yK :*:: \\ 7 "* ^.""7- ;^* : S-":\\ -."" CONTROL pH(S.U.) 4-uM -}.i? -*.So 7M\\ 1.70 3..3Z. -*4^ 7.^15 DO (mg/L) ^^ 14 -?.* l.<* 7.7 ^ ?** 7.7 Conductivity (umhos/cm) 30D ^:i>:#!'W; tffru* 3,3 3i5 'Alkalinity (mgCaC03/L) \\*% l^:Sf!:i'fe? ss 111113 V.v* ' r ^^'^4'>>':'* \\.v>>* ,C-';*, **'^;-
Hardncss (mgCaCOi/L)

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?.s^ ^32 7.37 DO (mg/L) ^ ^ -f.s 7.S 7.% V.3 o-o 7? Conductivity (umhos/cm) &z4
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Temperature C°C>
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T-02. T.UM-743 7.76 1.3Z. 771 737 DO (mg/L) w 7-1* 79 7? ^?-3 &i 7? Conductivity (umhos/cm) lo^o 'M^Sffl Ilio IIHO ""*'* 'i *;*%*']. mo
Tempcrature
(°C) -1>V1 TMb "bl-Vo -v>l-s
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600 mg KCI/L pH (S.U.) "V-fl?. "*.l+ T-.v^q. 7*f6 7.77 T.*sr ^-fe'?' 73* DO (mg/L) a3 T-.T-IX 7.? 7? ?.2 ff.l ?.o Conductivity (umhos/cm) fcio Bio M3Q §1113 mo r ^"-: ** * *.!
Temperature CO lA-1
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\\q.4 iH.-\\ 1H.S ~b\\.W -u\\.i-750 mg KCI/L pH(S.U) ^J&z ^.2\\ T-.
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^2 ff.v 9.0 Conductivity fotmhos/cm)
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^?H&9 7 fO DO (mg/L) QA T-.<^ ^s 9.0 K.O 1.o {?.<<. ?.X> Conductivity (umhos/cm) *alto M§ho i'.'-V-VJr-'-C'!: 2)2-0 2210 ^""'"*ii^&l'H ?/7<<? ^Temperature PC) t^i -L*\\.T-TH.U. TM-T-T.4-1 iH.i -lH.S Hu/ Initial Final Initial Final Initial Final Initial Final eraturesperformed at the time oftest initiation, renewalor terminationby the analyst identified in the Daily Renewal Infonnation tablelocatedon Page I. itynnd hardness pcrfonncd bythe analyst identified on the bench sheet specific for each analysis and transcribed tothis bench sheet by: X\\ SOP AT21 - Exhibit AT21.1, revision 11 14 Page 77 of 91 V. >>*. << :& !'j.t;vtfjjgviV. Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Source: In-house Culture Environmental Testing Solutions, inc. 1.12 1.10 1.08 h 1.06 u 03 z 1.04 -J 1.02 m CM u TO 1.15 rs 1.10 1.05 1.00 1 i I I i I I i 1 1 1 I 1 1 1 1 1 ' 1 T" Control Limits (+/- 2 Standard Deviations) 1 <<. <<- *"**-- ~-~ "*" *"~""""" *"* e © © © 0 o © £ © ^ w <<3*aa>> ^.^ ^^ Q ^_ ^ ^, _ _, _ _ _ '1 iiir iir ir i 1 1r Warning Limits 0.95^>>t7>>>>>>>>>>>>>>>>>* Test date 7-day IC^ =25%inhibition concentration. An estimation of the sodiumchlorideconcentration which would cause a 25%reduction in Ceriodaphnia reproduction (calculated usingToxCalc). Central Tendency (mean logarithmic IC,5 converted to anti-logarithmic values) Control Limits (mean logarithmic IC25 +/-2 standard deviations converted to anti-logarithmic values) Laboratory Warning Limits (mean logarithmic 1C25 +/-2 coefficent ofvariations converted to antf-logarithmic values) USEPA Warning Limits (mean logarithmic IC25 +/-S^10 converted to anti-logarithmic values, SA10 =10th percentile ofCVs reported nationally by USEPA) 1 Reviewed 6? Page 78 of 91 ^vmp iwy-Ceriodaphnia dubia Chronic Reference Toxicant Control Chart ^l Environmental TimlrtflSotiHtom. Inc. o s ource: iin-house C ulture Test number Test date 7-day IC2S Logi0 Conversion Anti-logarithmic Values (g/L NaCl) Laboratory Calculated CV 10th Percentile CV ToxCal Determination 7-day IC^ CT s cr Control Limits Warning Limits Warning Limits (g/LNaCi) CT-2S CT+2S CT-2CV CT + 2CV CT-SA,10 CT +S^ 1 05-02-17 1.0691 0.0290 0.0275 0.0028 1.0654 1.0516 1.0794 1.0525 1.0783 0.9802 1.1506 2 06-06-17 1.0614 0.0259 0.0276 0.0027 1.0657 1.0524 1.0791 1.0532 1.0781 0.9804 1.1509 3 06-06-17 1.0518 0.0219 0.0275 0.0029 1.0653 1.0511 1.0798 1.0520 1.0787 0.9801 1.1506 4 07-11-17 1.0631 0.0266 0.0275 0.0029 1.0654 1.0512 1.0798 1.0520 1.0787 0,9602 1.1506 5 08-08-17 1.0810 0.0338 0.0277 0.0032 1.0660 1.0502 1.0820 1.0512 1.0807 0.9807 1.1513 6 09-12-17 1.0527 0.0223 0.0274 0.0034 1.0650 1.0485 1.0818 1.0495 1.0805 0.9798 1.1502 7 10-03-17 1.0540 0.0228 0.0272 0.0035 1.0646 1.0474 1.0821 1.0484 1.0807 0,9794 1.1497 8 11-07-17 1.0528 0.0223 0.0270 0,0037 1.0642 1.0463 1.0824 1.0473 1.0810 0.9790 1.1493 9 12-05-17 1.0469 0.0199 0.0269 0.0039 1,0638 1.0449 1.0832 1.0460 1.0817 0.9787 1.1489 10 01-09-18 1.0826 0.0345 0.0274 0.0042 1.0651 1.0446 1.0859 1.0458 1.0843 0.9799 1.1503 11 02-06-18 1.0500 0.0212 0.0270 0.0044 1.0642 1.0427 1.0862 1.0440 1.0844 0.9791 1.1494 12 03-06-18 1.0697 0.0293 0.0272 0.0045 1.0646 1.0430 1.0867 1.0443 1.0849 0.9794 1.1498 13 04-03-18 1.0835 0.0348 0.0273 0.0047 1.0649 1.0424 1.0880 1.0437 1.0861 0,9797 1.1501 14 04-03-18 1.0827 0.0345 0.0274 0.0048 1.0651 1.0420 1.0888 1.0434 1.0869 0.9799 1.1503 15 05-08-18 1.0822 0,0343 0.0275 0.0049 1.0655 1.0415 1.0899 1.0430 1.0879 0.9802 1.1507 16 06-05-18 1.0696 0.0292 0.0275 0.0049 1.0653 1.0415 1.0897 1.0430 1.0877 0.9801 L1506 17 07-10-18 1,0694 0.0291 0.0276 0.0049 1.0656 1.0417 1.0900 1.0432 1.0880 0.9804 1.1508 18 08-07-18 1.0555 0.0235 0.0274 0.0050 1.0652 1.0409 1,0900 1,0424 1.0879 0.9800 1.1504 19 09-11-18 1.0682 0.0287 0.0276 0.0050 1.0655 1.0413 1.0903 1.0428 1.0883 0.9803 1.1508 20 10-09-18 1.0640 0,0269 0.0275 0.0050 1.0654 1.0412 1.0902 1.0427 1.0882 0.9802 1.1507 Note: 7-day IC25 = 25%inhibition concentration. Anestimation of the sodium chloride concentration that would cause a 25%reduction in Ceriodaphnia reproduction (calculated using ToxCalc). CT= Central tendency of the ICW values. 5 - Standard deviation of the IC25 values. Control Limits = Mean logarithmic 1C25 +/- 2 standard deviations converted to anti-logarithmic values. Warning Limits = Mean logarithmic ICj*+/- 2CVor SA,G converted to anti-logarithmic values. sa.io - Siandatd deviation corresponding to the 10* percentile ofCVs reported nationally byUSEPA (SM0 =0.08). CV = Coefficient of variation. Micro? **>>} I<<wi<<WBfnut TMUQ9SontUcn%,tru. Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture 40 £-35 o CD tt 03 3 e "O Cl) o H- ^^ a ^^ 0J bo or c Jb-o a u. </> +*>> c o o u 7 1 o 1 © 1 1 I I I l 1 1 i 1 1 0 <<~-~<<>> <<..u<<w ^...*-,<<>> <<<<<<<<. 1 l 1 o © 1 i USEPA Minimum Acceptance Criteria (> 15 offspring per surviving female) © 1 2 I 111)1 1 1 1 1 1 1 I 1 1 1 1 1 1 P t5 f +- o C +/-; o o o ,2 Q in a. 30 b 25 20 15 10 l I i 1 I 1 i 1 1 1 1 1 1 1 I j 1 l l 40 USEPA Maximum Coefficient of Variation Guidance Criteria (< 42%), NCDEQ Maximum (<40%) 30 20 10 © .*<<, *->>, <<=>> ^jg>> -*>>*>>*
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<<<<* *a>> <<*^ *>>>> <<"*>> v.*, xra. ^ ^ ^^ <<_,.^>> ~ 0 l 1 1 50 30 20 10 Test date Control Reproduction, Coefficient of Variation (CV)or Percent Minimum Significant Difference (PMSD) PMSDis the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSDbound represents a practical limit to the sensitivity of the test method and is not a minimum acceptance criteria. Central Tendency (mean Control Reproduction, CV or PMSD) 95% Confidence Interval (mean Control Reproduction, CV or PMSD +/- 2 Standard Deviations) Page 80 of 91 1 1 1 I 1 1 1 1 1 I 1 1 1 1 1 1 1 1 1 1 USEPA Upper PMSD Bound Acceptance Criteria (< 47%) USEPA Lower PMSD Bound (>13%)
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Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture o -A Test number Test date ToxCal Determination Control Reproduction (offspring/female) Control Reproduction CV m Test PMSD 1%) Control Survival Control Reproduction Test Mean CV MSD PMSD CT 95% Confidence Interval CT 95% Confidence Interval CT 95% Confidence Interval (%) (offspring/female) (%) (%) CT - 2S CT + 2S CT-25 CT + 2S CT-2S CT + 2S 1 05-02-17 100 31.0 5.9 1.894 6.1 30.2 27.4 32.9 5.1 2.3 8.0 5.8 4.3 7.3 2 06-06-17 100 30.7 4.9 1.968 6.4 30.2 27.4 32.9 S.2 2.5 7.9 5,9 4.6 7.3 3 06-06-17 100 33,1 4.6 2.050 6.2 30.2 27.3 33.2 5.1 2.5 7.7 5.9 4.6 7.3 4 07-11-17 100 31.0 4.8 1.661 5.4 30.2 27.3 33.1 5,0 2.5 7,6 5.9 4.6 7.3 5 08-08-17 100 28.7 4.7 1.668 5.8 30.1 27.2 33.1 5.0 2.4 7 5 5.9 4.6 7.3 6 09-12-17 100 30.3 6.8 2.191 7.2 30.2 27,3 33.1 5.0 2.4 7.7 6.0 4.5 7.4 7 10-03-17 100 32.2 3.5 1.926 6.0 30.2 27.3 33.1 4.9 2.2 7,7 6.0 4.6 7.4 8 11-07-17 100 32.3 7.4 2.21S 6.9 30.2 27.3 33.2 5.1 2.3 8.0 6.1 4.7 7.5 9 12-05-17 100 28.6 6.0 2.344 8.2 30.3 27,4 33.1 5.2 2.5 8.0 6.2 4.5 7.9 10 01-09-18 100 29.7 4.5 2.015 6.8 30.1 27.4 32.8 5.2 2.5 8.0 6,3 4.7 7.9 11 02-06-18 100 29.3 5.6 1.824 6*2 30.1 27.4 32.8 5.2 2.5 7 9 6.2 4.7 7.8 12 03-06-18 100 30.6 6.8 1.995 6.5 30.1 27.4 32.8 5.2 2.4 8.0 6.2 4.7 7.8 13 04-03-18 100 28.0 4.5 1.729 6.2 30.1 27.3 32.9 5.3 2.6 7.9 6.2 4.7 7.8 14 04-03-18 100 31.8 5.5 2.108 6.6 30.2 27,4 33.1 5.3 2,7 7.9 6.3 4.7 7.8 IS 05-08-18 100 30.3 5.4 2.172 7.2 30.3 27.5 33.1 53 2.7 8,0 6.4 4.9 7.8 16 06-05-18 100 31.5 5.6 2.469 7.8 30.3 27.5 33.2 5.5 3.0 79 6.5 5.0 8.0 17 07-10-18 100 28.8 4.9 1.598 5.5 30.4 27.7 33.1 5.3 3.3 7.2 6.4 4.9 8.0 18 08-07-18 100 31,3 3.0 1.806 5.8 30.5 27.7 33.2 5.2 3,1 7.4 6,5 5.0 7.9 19 09-11-18 100 29.9 5.8 1.943 6.5 30.5 27.7 33.2 5.3 3.1 7,4 6,5 5.0 7.9 20 10-09-18 100 29.5 8.2 1.912 6.5 304 27.7 33.2 5.4 2.9 7.9 6.5 5.1 7.9 Note: Control Survival - USEPA minimum test acceptability criteria > 80% survival. ControlMean Reproduction<< USEPA minimum test acceptability criteriaa is offspring/surviving female CV= Coefficient of variation for control reproduction USEPA maximum CV guidance criteria (90,h percentile) <42%. NCDfTQ maximum CV acceptance criteria <40%. MSD = Minimum significant difference. PMSD = Percent minimum significant difference. PMSD isa measure of test prebsion, ThePMSD isthe minimum percentdifferencebetween the controland treatment that can be declaredstatistically significant Ina wholeeffluenttoxicity test. Lower PMSD bound determined by USfcPA {10,h percentile) >13%. Thelower PMSD bound represents a practicallimitto the sensitivityof the test method and isnot a minimumacceptance criteria Upper PMSD bound acceptance criteria determined byUSEPA (9Q,H percentile} c 47%. CT << Central tendency of the reproduction, CVor PMSD values. S - Standard deviation of the reproduction. CVor PMSD values. tf _>> la<<lronmewUJTrtth>95efta>>><<H<<c Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphma dubia Page I of6 CdNaCICR#: 220 Dilutionpreparation information: Comments: NaCl Stock INSS number inss r\\c& Stock preparation: lOOgNaCi/L: Dissolve 50 g NaCl in 500 mL Milli-Q water. Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 IS 21 Diluent volume (mL) 1491 I4S8 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test organism source information: Test information: Organism age: < 24-hours old Randomizing template color: ftvAWL Dateand times organisms were bom between: io-O^IS' c*>>vS "TO fitoS Incubator number and shelf location: 19>\\ Culture board: IO-CVl£ tK Replicate number: Culture board cup number. i 2 5 j 4 5 6 7 s 9 to YWT batch: U-OHf 1 JO n Ivx n K "U>> xs ao 31 Transfer vessel information'. pH=n.£S S.U. Temperature = -\\&*\\CC Setenasdum batch: J.0>>ffU\\f Average transfer volume (mL): < 0.25 mL Daily renewal information: Day Date Test initiation and feeding, renewal and feeding, or termination time MHSW batch used Analyst 0 10-09-18 olMl ivca-iS A 1 10-10-18 oJSb la-rji-ti J 2 10-11-18 oH& la-crv* i £ If i 0 10-12-18 0*41 lO-ff\\-V& \\\\ 4 10-13-18 a^S ro-iH* fc u A 5 10-14-18 0££t> io-iY-\\4 rs ft 6 10-15-18 o&4Sr IO-\\\\-\\ & (\\ 7 10-16-18 oHf '.v-'l-.IU.iVfiia7. '*'Z.'j -ii Control information: Acceptance criteria Summary oftest endpoints: % of Male Adults: 01. 520% 7-day LCso >l4cr\\ % Adults having 3rd Broods: 1007. > 80% NOEC tocn % Mortality: 07. <20% LOEC tioo Mean Offspring/Female: 7ft J^ > 15.0offspring/female ChV Itftt.S % CV: Mr* <40.0% Ids IPVf- ° Page 82 of 91 SOP ATM - Exhibit ATI4.1, revision I t-OI-14 'J. ill it<Y; O If Page 2 of6 , trwtnflrMnulTmtiagScttMMtwtm. Species: Ceriodaphnia dubia CONTROL Day 1 Young produced Adult mortality -O-u \\_ Young produced Adult mortality ^ Young produced Adult mortality XL o Young produced Adult mortality Young produced ID Adult mortality v_ Young produced Adult mortality u J^L Young produced \\K* lA. Total young produced ^^
>b Final Adult Mortality X for 3rd Broods V
X yg. CdNaCICR#: 220 Survival and Reproduction Data Replicate number 10 ja \\- v_H. -CL v_ V-c V V_ V_- XL o JS. -Q_ O M lT_ _fL IO IO >0 \\V
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t\\o !>S I"I 5>o i3 lu, U T>2-14 x* 1+/- T-V T-S "l* TT" V-s FTT^ JS*. -^ A'ott-Adultmortality (L=live,D=dead), SB=splitbrood (sinsle brood splitbetween twodays), CO=canyover(offspring carried over with adult during transfer). H. 600 mg NaCl/L Day Young produced Adult mortality jCl U _a Young produced Adult mortality Young produced Adult mortality l_ Young produced Adult mortality V. Young produced 10 Adult mortality Young produced Adult mortality o v_ £l Young produced \\\\o tu Total young produced 1f\\ "2-<\\ Pinal Adult Mortality Concentration: % Mortality: Ol. Mean Offspring/Female: ~U\\& Survival andReproduction Data Replicate number 10 o O u V^ a _A L_ a O L_ L. v_ o O U V_ U t> >>1s to >l '0 IL. iO XL XL _Cl _£l XL >>"b lU \\u>> VS IS. V2>> 15 "2A bo
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15 1.9 ii 5o _L_ Note: Adultmortality (L=live,D= dead), SB =splitbrood fsingle brood splitbetween twodays), CO=carry over(offspring carried over with adult during transfer). Page 83 of 91 Concentration: % Mortality: Ot Mean Offspring/Female: -2<<V<=> % Reduction from Control: - 0.-& 7 SOP AT14-Exhibit AT14.1, revision 11-0M4 iet1 J lavtoa&a>eftUttratika.tofixie<tfctac Species: Ceriodaphnia dubia 800 mg NaCI/L Day 1 Young produced Adult mortality l_ Jd Young produced o _D_ Adult mortality v_ Young produced Adult mortality XI _D_ Young produced q Adult mortality Young produced u v\\ Adult mortality Young produced Adult mortality o XL Young produced i4 >>T_ Total young produced x°\\ -L°\\ Final Adult Mortality Page 3 of6 CdNaCICRJ: 220 Survival and Reproduction Data Replicate number 10 XI O _Q_ o o XL \\_ D XL 0 _D_ _o XL o v_ IV tfc ro IO IO v XL i4
S i^.
vS \\Vo l"L i4 ll
50 "U
"2>0 Tsl ^st> TJio IS -tf Note: Adultmortality (L~ live, D-dead), SB - split brood (single brood splitbetween twodays), CO>> carry over (offspring carried overwith adult duringtransfer). Concentration: % Mortality: 07. Mean OfTspring/Female: Tft.V % Reduction from Control: \\.4 7. 1000 rag NaO/L Survival andReproduction Data Day Replicate number 10 Young produced Adult mortality -£ XX V. O o L_ o o Young produced Adult mortality XI O XL XL L_ JX XL ^ Young produced Adult mortality ^ n H. XL v__ XL U
t o
o XI Young produced Adult mortality v^ v_ Young produced JQ to IO il IO l-L II Adult mortality Young produced Adult mortality XL V^ JX \\^ O Young produced Total young produced Final Adult Mortality ^ 1*\\ 1^ IX ir\\ i<> i4 ho 13* 1% IS IS IS 1°k ii ^O c Note: Adultmortality (L =live, D-dead), SB =splitbrood (single brood splitbetween two days),CO* carry over(offspring carried over with adult during transfer). Page 84 of 91 Concentration: % Mortality: 07. Mean Offspring/Female: XI.7 % Reduction from Control: U7. SOP AT14-Exhibit AT14.1, revision 11-01-14
fr^i^fr1 Paae 4 of 6
? £miwmm*cUtTe>>PngW*j>>to<<*f<t£. Species: Ceriodaphma dubia 1200mgNaCI/L CdNaCICRfc 220 Survival and Reproduction Data Day Young produced Adult mortality Young produced Adult mortality Young produced Adult mortality Young produced Adult mortality Young produced Adult mortality Young produced Adult mortality Young produced Total young produced Final Adult Mortality Replicate number 10 _Q_ Xl XL o XI \\_ o O O O XL XL XX n JQ_ J2l o O O V_
A
+/-L +/-L ^T G o o _ 5 Young produced o -L-O o O 0 0 \\ 0 O Adult mortalUy u \\ v_ V v_ v_ v_ v_ u. \\_ 6 Yonng produced o 0 0 0 0 O a 6 6 0 Adult mortality u V v_ v_ \\_ V_- ^ v_ v_ v_ 7 Young produced 0 0 o 0 0 0 6 0 0 0 Total youtig produced \\ "L 7_ I i ^ I \\ X. T_ Final Adult Mortality V V- \\_ \\_ \\ \\ v_ v ^_ v^ Note: Adult mortality (L= live, D= dead), SB<< splitbrood (single brood splitbetween twodays), CO~ carry over(offspring earned over with adult during transfer). Page 85 of 91 Concentration: % Mortality: m Mean Offspring/Female: l.W % Reduction from Control: 4M.fc7. SOP ATI4-Exhibit ATI4.1, revision li-Ol-14 mi 2
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o UI Jbk o o o 00 u in a to H o o o 00 VI s o O in o o o 10 u v>> o Ui o o a to ID o ->j o M a o o s UJ o UJ o ro in o o o s g UJ ^4 oa UJ in o o o g ff y M o o4^ UJ to o o o o1 E o o o 3 OX) OJ n o Qj 5' 3 O-ti I? Q I 3 Oa. c ft o' 3 H O i-t-OJ ro oo o to Envlronment.ilTesting Solutluns. Inc. Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number? Test dates: CdNaCICR<<220 October 09-16,2018 Concentration (mE/LNaC!) Replicate number Survival Average reproduction (offspring/female) Coefficient of variation [%) Percent reduction from control (%) 1 2 3 4 5 5 7 a 9 10 Control 29 33 33 30 22 32 28 28 ?8 7g 1.00 29.5 8.2 Not applicable 600 29 29 31 30 29 32 29 28 29 30 100 29.6 4,0 -0.3 800 29 29 30 28 30 35 30 26 28 29 100 29.1 5.5 1.1 1000 29 2b 27 23 30 25 28 28 30 100 27.7 6.8 6.1 1200 10 8 9 23 H 10 g 7 15 9 100 10.a 25.7 61.7 1--100 2 2 1 3
j i
r 7 j 100 1.6 13,7 94.6 Dunnett's MSD value: PMSD: .912 USEPA. 2001a, 2001b Environments! Protect Final Rapor ion Agency t; Inteflaboratory Cincinnati. OH MSD = Minimum Significant Difference PMSD-Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant In a whole effluent toxicity test. LowerPMSD bound determined by USEPA (10 percentile) = 13%. UpperPMSD bounddetermined byUSEPA (90"' percentile) =47%. Lower and upper PMS0 bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA'sWET interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b), The lower PMSDbound represents a practical limit to the sensitivity of the test method and Is not a minimum acceptance criteria. VariabilityStudy of EPAShort-term Chronic and Acute Whole Effluent ToxicityTest Methods, Volumes 1 and 2-AoDendix. EPA-S21-B-01-004 and EPA-821-B-Q1-005. us 2 Statistical Analyses Envfronrrxmtal letflng SoluMenv fnc Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 10/9/2018 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Dale: 10/16/20ie Lab ID: ETS-Envir. Tasting Sol Sample Type: NACL-Sodium chloride Sample Dale: Protocol FWCMR-EPA-821-R-02-Q13 Test Species: CD-Cenodaphnia dubia Comments: Cortc-mg/L 1 2 3 4 5 6 7 8 9 10 D-Control 29-000 33.000 33.000 30.000 26.000 32.000 28.000 28.000 28.000 28,000 600 29.000 29.000 31,000 30.000 29 000 32000 29.000 28.000 29.000 30.000 800 29.000 29 000 30.000 28.000 30,000 32,000 30.000 26,000 28.000 28,000 1000 29.000 26.000 27.000 25.000 30.000 25,000 28.000 28,000 29.000 30.000 1200 10.000 8.QG0 9.000 13.000 14.000 10.000 9.000 7.000 15000 9.000 1400 1.000 2.000 2.000 1.000 1 000 3.000 1.000 1.QQ0 2.000 2.000 Conc-mg/L Mean N-Mean Mean D-Control 29,500 10000 29.500 26.000 33.000 8.187 10 600 29.600 10034 29.600 28.000 32.000 3.965 10 800 29.100 0,9864 29.100 26.000 32.000 5.482 10 1000 27 700 Q.9390 27.700 25.000 30.000 6.818 10 Transform: Untransformed Min Max CV%
1200 10.400 0.3525 10.400 7.000 15.000 25,721 N
10
1400 1.600 0.0542 1.600 1.000 3.000 43.700 10 Rank Sum 1-Tailed Critical Isotonic Mean N-Mean 29.550 1.0000 113.00 75.00 29.550 1.0000 105.00 75.00 29.100 0.9848 86.50 75.00 27.700 0.9374 55.00 75.00 10.400 0,3519 55.00 75.00 1.600 0.0541 Auxiliary Tests Statistic Critical Skew Kurt Koimogorov D Test indicates normal distribution (p > 0.01)
Bariiett's Test indicates unequal variances (p = 4.59E-03) 0.87486 16.954 1.035 15.0863 041935 0.0382 Hypothesis Test (1-tatt, 0.05) NOEC LOEC ChV TU Steel's Many-One RanK Test Treatments vs D-Controi 1000 1200 1095.45 Point mg/L SD Linear Interpolation (200 Resamples) 95% CL Skew IC05 946.786 102.278 673J23S 10D8.67 -1.5854 IC10 1012.77 21 5456 930.85 1025.48 -2 8515 IC15 1029.86 8.05625 1012.26 1042-05 -0.2255 IC20 1046.94 7.60606 1030.73 1058.6 -0.2148 IC25 1064,02 7 31883 1048 23 1075.01 -0.2050 IC40 1115.26 7.56893 1098 97 1129.87 0,0321 IC50 1149.42 857853 1131.79 1167.31 0 3597 Dose-Response Plot Page 88 of 91 1000 Dose mg/L ^ Statistical Analyses Environmental Testing Solutions, Inc. Used for PMSD calculation only. Start Date: End Date: Sample Date: Comments: Ceriodaphnia Survival and Reproduction Test-Reproduction 10/9/2018 Test ID: CdNaCICR Sample ID: 10/16/2018 Lab ID: ETS-Envlr. Testing Sol. Sample Type: Protocol: RA/CHR-EPA-821^R-02-013 Test Species: REF-Ref Toxicant NACL-Sodium chloride CD~Ceriodaphnia dubia Conc-mg/L D-Control 600 800 1000 1200 1400 29.000 29.000 29.000 29.000 10.000 1.000 33.000 29.000 29.000 26.000 8.000 2.000 33.000 31 000 30 000 27.000 9.000 2.000 30.000 30.000 28.000 25.000 13.000 1.000 26.000 29.000 30.000 30.000 14.000 1.000 32.000 32.000 32.000 25.000 10.000 3.000 Transform: Untransformed Conc-mg/L Mean N-Mean Mean D-Control 600 800 1000
1200

1400 29.500 29.600 29.100 27.700 10.400 1.600 1.0000 1.0034 0.9864 0.9390 0.3525 0.0542 29.500 29.600 29.100 27.700 10.400 1.600 Min 26.000 28.000 26.000 25.000 7.000 1.000 Max 33.000 32 000 32.000 30,000 15.000 3000 CV%
8.187 3.965 5.482 6.818 25.721 43.700 28.000 29.000 30.000 28.000 9.000 1.000 10 10 10 10 10 10 8 28.000 28.000 26.000 28.000 7.000 1.000 28.000 29.000 28.000 29.000 15.000 2.000 t-Stat 1-Tailed Critical -0.120 0.478 2.153 22.847 33.373 2.287 2.287 2.287 2.287 2.287 10 28.000 30.000 29.000 30,000 9.000 2.000 MSD 1.912 1.912 1.912 1,912 1.912 Auxiliary Tests Statistic Critical Skew Kurt Koimogorov D Test indicates normal distribution (p > 0.01) Bartiett's Test indicates unequal variances (p = 4.59E-03) 0.87486 16.954 1.035 15.0863 0.41935 0.0382 Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 1000 1200 1095.45 Treatments vs D-Control 1.91164 0.0648 1489.66 3.49444 4.4E-42 5.54 ^ Page 89 of 91 f7UET^j^3 Page 5 of6 . (<utfBMneiain<<B*gfott!U>AtIac Species: Ceriodaphnia dubia CdNaCICR #: 220 Daily ChemistryK Day (Analyst identified foreach day, performedpH, D.O. and conductivity measurementsonly.) 0 1 2 Analyst MS MS MX TS TS I/O Concentration Parameter SSisSf; WrlWa PfSPS! mum !?lliillSll CONTROL pH (S.U.) ?&<>> ^.8*t "=V=fs 7.87 7.59 <U*^ DO (mg/L)
7-?
l.<3 ^0 &o 7-9 aPl Conductivity (pmhos/cm) 311 3/t* 30A
Alkalinity (mgCaCOs/L)
<dO V* <><\\
Hardncss (mgCaC03/L)

4

^7".V"^4'; -v So

Temperature (°C) i.<<U8
-6.o -*\\A' X4-*
x\\.\\+
-\\it* 600 mg NaCI/L pH (S.U.) ^8\\
3-ff-</
^-s*? 7M 777 Ci^-v DO (mg/L) l.e ^fe %.o 7? A^ Conductivity (umhos/cm) Wo tS2o
%^rif^
~r. mo <<L'i#fe<<uJV^ ^Temperature (*C) -uM T.AA -^.8 TA-i "MI-Vd -w-<<\\ 800mgNaCI/L pH (S.U.) 3-SZ itg"/ "^.fffi 7.81 7.?0 w DO (mg/L) 18 7.<? 1J.0 9.0 -7.9 ©*o Conductivity (jimhos/cm) ie&o fJ*' ^ ~ /fl>>0 HAG ^Temperature (°C) j TH-f ^-^ I -l<L1
\\<~A
^*1 x*VA lOOOmgNaCl/L pH(S.U) \\^f>Z y.Bi ~7.tf? 7.2S 723 ^(^ DO (mg/L) ^f ^<? ^<F &./ 79 0.O Conductivity (umhos/cm) Z/So 1Z3o f:m^>.2/6(3
Temporaturc (°C)

LH.g tsL*
TAA
v>.o xA-n TS.D l20OmgNaCl/L pH(S.U)
¥.ffZ Tr!*£T %8<i T23 7 J?.? ^U-0 DO (mg/L) ^-7 \\<* ^<? S./ 7.9 ©.o Conductivity (umhos/cm) 2&>oo \\*&f$'t Z660 c^O
Temperature (°C)

V*,£

vl-1
"\\S-X) WA
fctfe
-utg 1400 ragNaCl/L pH (S.U.) ^8H "=?.*& 7.?l 7.BH 7Z4 a^2_ DO (mg/L) 8~o 7.<r S.l 7.9 54 Conductivity (umhos/cm)
2990
^'*$*'*^3 Z&lo ^Mii 3&-70 ^Temperature (°C) -rt-f M-4 "is-c. t\\.s
yl.l nH.*\\
I Initial l Final Initial Pinal Initial Final Temperaturesperformed atihctimeof testinitiation, renewal ortermination bytheanalyst identified indieDaily Renewal Informauon table located onPage 1. Alkalinity and ha/dness performed bythe analyst identified onihc bench sheet specific for each analysis and transcribed to this benchsheet by: Page 90 of 91 SOP ATI4 - Exliibit ATI4J, revision 11-0M4 s^ Page 6 of6 . tn>>Jjo<<racnUfT<<tagSofefttoitvt>>c. Species: Ceriodaphnia dubia CdNaCICR #: 220
Tcmpcraturcs performed at the time oftesi initiation, renewal or termination bythe analyst identified inihc Daily Renewal Information table located on Page 1. Alkalinity and hardness performed bythe analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:
\\/\\ Page 91 of 91 SOP ATM - Exliibit ATI4.I, revision 11-01-I4

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