ML17124A128

2016 Annual Environmental Operating Report (Non-Radiological)

ML17124A128
Person / Time
Site:	Beaver Valley
Issue date:	02/16/2017
From:	Lange C L FirstEnergy Nuclear Operating Co
To:	Office of Nuclear Reactor Regulation
References
L-17-126
Download: ML17124A128 (82)

Text

FIRSTENERGY NUCLEAR OPERATING COMPANY BEA VER VALLEY POWER STATION 2016 ANNUAL ENVIRONMENTAL OPERATING REPORT NON-RADIOLOGICAL UNITS NO. 1 AND 2 LICENSES DPR-66 AND NPF-73 RTL# A9.630F

5.1.1 BEAVER

VALLEY POWER STATION ENVIRONMENTAL

& CHEMISTRY SECTION Technical Report Approval 2016 ANNUAL ENVIRONMENTAL OPERATING REPORT (Non-Radiological)

UNITS NO. 1 AND 2 LICENSES DPR-66 AND NPF-73 Prepared by: Cameron L. Lange (Via E-mail) Date: 2-16-17 Reviewed by: Courtney F.

Date:

Reviewed by: Susan L. Vicinie Date: 1-lu -11 Approved by: Donald J. Salera Date: +£r/1 TABLE OF CONTENTS 1.0 EXECUTIVE

SUMMARY

............................................................................................

1

1.1 INTRODUCTION

.................

..............................................................................

1 1.2

SUMMARY

& CONCLUSIONS

........................................................................

.2 1.3 ANALYSIS OF SIGNIFICANT ENVIRONMENTAL CHANGE .....................

2 1.4 AQUA TIC MONITORING PROGRAM EXECUTIVE

SUMMARY

................

2 2.0 ENVIRONMENTAL PROTECTION PLAN NON-COMPLIANCES

....................

.4 3.0 CHANGES INVOLVING UNREVIEWED ENVIRONMENTAL QUESTIONS

.. .4 4.0 NONROUTINE ENVIRONMENT AL REPORTS ....................................................

.5 5.0 AQUATIC MONITORING PROGRAM .....................................................................

6 5.1 SITE DESCIPTION

................................................

.-.............................................

6 5.2

• STUDY AREA .....................................................................................................

7 5.3 METHODS ...................................................................

-....................................

7 5.3.1 Benthic Macroinvertebrate Monitoring

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8 5 .3 .2 Fish Monitoring

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8 5.3.3 Corbicula!Zebra Mussel Density Determinations

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9 5.3.4 Corbicula Juvenile Monitoring

...............................................................

10 5.3.5 Zebra Mussel Monitoring

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.11 5.3.6 Reports ....................................................................................................

12 5.4 AQUATIC MONITORING PROGRAM AND RESULTS ...............................

12 5.4. l Benthic Macroinvertebrate Monitoring Program ....................................

12 5.4.2 Fish Sampling Program ...........................................................................

16 5.4.3 Corbicula Monitoring Program ..............................................................

19 5.4.4 Corbicula Juvenile Monitoring

...............................................................

20 5.4.5 Zebra Mussel Monitoring Program .........................................................

21 6.0 ZEBRA MUSSEL AND CORBICULA CONTROL ACTIVITIES

.........................

.23

7.0 REFERENCES

..............................................................................................................

24 8.0 TABLES 9.0 FIGURES 10.0 PERMITS APPENDIX A. SCIENTIFIC AND COMMON NAME OF FISH COLLECTED IN THE NEW CUMBERLAND POOL OF THE OHIO RIVER, 1970 THROUGH 2016 BVPS 2016 Annual Environmental Report FENOC (BVPS)

LIST OF TABLES 5 .1 Beaver Valley Power Station (BVPS) Sampling Dates for 2016. 5 .2 Systematic List of Macroinvertebrates Collected From 1973 through 2016 in the Ohio River near BVPS ( 6 sheets). 5.3 Benthic Mac.roinvertebrate Counts for Triplicate Samples Taken at Each Sample Station by Sample for May and September 2016. 5.4 Mean Number of Macroinvertebrates (Number/m 2) and Percent Composition of Oligochaeta, Chironomidae, Mollusca and Other Organisms, 2016, BVPS. 5.5 Mean Number of Macroinvertebrates (Number/m 2) and Percent Composition of Oligochaeta, Chironomidae, Mollusca and Other Organisms for the Control Station (1) and the Average for Non-control Stations (2Bl, 2B2, and 2B3), 2016, BVPS. 5.6 Shannon-Weiner Diversity, Evenness and Richness Indices for Benthic Macroinvertebrates Collected in the Ohio River, 2016. 5.7 Benthic Macroinvertebrate Densities (Number/m 2) for Station 1 (Control) and Station 2B (Non-Control)

During Preoperational and Operational Years through 2016 BVPS. 5.8 Total Fish Catch, Electrofishing and Seine Net Combined During the BVPS 2016 Fisheries Survey. 5.9 Comparison of Control vs. Non-Control Electrofishing Catches, During the BVPS 2016 Fisheries Survey. 5 .10 Fish Species Collected During the May 2016 Sampling of the Ohio River in the Vicinity of BVPS. 5 .11 Fish Species Collected During the July 2016 Sampling of the Ohio River in the Vicinity of BVPS. 5.12 Fish Species Collected During the September 2016 Sampling of the Ohio River in the Vicinity of BVPS. 5.13 Fish Species Collected During the November 2016 Sampling of the Ohio River in the Vicinity of BVPS. 5.14 Estimated Number offish Observed During Electrofishing Operations, 2016. 5.15 Catch per Unit of Effort (CPUE as Fish/Electrofishing Minute) by Season during the BVPS 2013 Fisheries Survey. 5.16 Catch per Unit of Effort (CPUE as Fish/Electrofishing Minute) by Season during the BVPS 2014 Fisheries Survey. 2016 Annual Environmental Report FENOC (BVPS) ii I LIST OF TABLES . 5.17 Catch per Unit of Effort (CPUE as Fish/Electrofishing Minute) by Season during the BVPS 2015 Fisheries Survey. 5.18 Catch per Unit of Effort (CPUE as Fish/Electrofishing Minute) by Season during the BVPS 2016 Fisheries Survey. 5.19 Unit 1 Cooling Reservoir Monthly Sampling Corbicula Density Data for 2016 fromBVPS.

5.20 Unit 2 Cooling Reservoir Monthly Sampling Corbicula Density Data for 2016 fromBVPS.

2016 Annual Environmental Report FENOC (BVPS) iii LIST OF FIGURES 5.1 Location Map for the 2016 Beaver Valley Power Station Aquatic Monitoring Program Sampling Control and Non-Control Sampling Stations.

5.2 Location

Map for Beaver Valley Power Station Benthic Organism Survey Sampling Sites for the 2016 Study. 5.3 Location Map for Beaver Valley Power Station Fish Population Survey Fish Sampling Sites for the 2016 Study. 5.4 Location of Study Area, Beaver Valley Power Station Shippingport, Pennsylvania BVPS. 5.5 Comparison of Live Corbicula Clam Density Estimates Among BVPS Unit 1 Cooling Tower Reservoir Sample Events for Various Clam Shell Size Groups, 2016. 5.6 Comparison of Live Corbicula Clam Density Estimates Among Unit 2 Cooling Tower Reservoir Sample Events for Various Clam Shell Size Groups, 2016. 5.7 Comparison of Live Corbicula Clam Density Estimates Among Intake Structure Sample Events for Various Clam Shell Size Groups, 2016. 5.8 Water Temperature and River Elevation Recorded on the Ohio River at the BVPS Intake Structure, During Monthly Sampling Dates, 2016. 5.9 Density of Zebra Mussel Veligers (#/m 3) Collected at Beaver Valley Power Station; Intake Structure, Unit 1 Cooling Tower Reservoir and Unit 2 Cooling Tower Reservoir, 2016. 5.10 Density of Zebra Mussel Veligers (#/m 3) Collected at Beaver Valley Power Station; Barge Slip, Splash Pool and Emergency Outfall Basin, 2016. 5.11 Density (#/m 2) of Settled Zebra Mussels at Beaver Valley Power Station; Intake Structure, Unit 1 Cooling Tower Reservoir and Unit 2 Cooling Tower Reservoir, 2016. 5.12 Density (#/m 2) of Settled Zebra Mussels at Beaver Valley Power Station; Barge Slip, Splash Pool and Emergency Outfall Basin, 2016. 2016 Annual Environmental Report FENOC (BVPS) IV

1.1 INTRODUCTION

This report is submitted in accordance with Section 5.4.1 of Appendix B: To Facility Operating License No. NPF-73, Beaver Valley Power Station Unit 2, Environmental Protection Plan (Non-Radiological).

Beaver Valley Power Station (BVPS) is operated by FirstEnergy Nuclear Operating Company (FENOC). The Objectives of the Environmental Protection Plan (EPP) are to:

• Verify that the facility is operated in an environmentally acceptable manner, as established by the Final Environmental Statement-Operating License Stage (FES-OL) and other Nuclear Regulatory Commission (NRC) environmental impact assessments,

• Keep plant operations personnel appraised of changes in environmental conditions that may affect the facility,

• Coordinate NRC requirements and maintain consistency with other Federal, State, and local requirements for environmental protection, and

• Keep the NRC informed of the environmental effects of facility construction and operation and of actions taken to control those effects. To achieve the objectives of the EPP, both FENOC and BVPS have written programs and procedures to comply with the EPP, protect the environment, and comply with governmental requirements primarily including

• the US Environmental Protection Agency (EPA) and the Pennsylvania Department of Environmental Protection (PADEP) requirements.

Water quality matters identified in the Final Environmental Statements-Operating License Stage (FES-OL) are regulated under the National Pollutants Discharge Elimination System (NPDES) Permit No. PA0025615.

Waste is regulated under EPA Identification No. PAR000040485.

Attachment 10.1 contains a listing of permits and certificates for environmental compliance.

The BVPS programs and procedures include pre-work and pre-project environmental evaluations, operating procedures, pollution prevention and response programs procedures and plans, process improvement and corrective action programs, and human performance programs.

Technical and managerial monitoring of tasks, operations, and other activities are performed.

Any identified challenges, concerns, or questions are captured in the FENOC Problem Identification and Resolution Program with a Condition Report. Condition Reports include investigations, cause determinations, and corrective actions. During 2016, BVPS continued an Aquatic Monitoring Program to evaluate its potential impact on the New Cumberland Pool of the Ohio River and to provide information on potential impacts to BVPS operation from macrofoulers such as Asian clams and zebra mussels. 2016 Annual Environmental Report FENOC (BVPS) 1.2

SUMMARY

AND CONCLUSIONS There were no significant environmental events during 2016. During 2016, no significant changes to operations that could affect the environment were made at Beaver Valley Power Station. As in previous years, results of the BVPS environmental programs did not indicate any adverse environmental impacts from station operation.

1.3 ANALYSIS

OF SIGNIFICANT ENVIRONMENTAL CHANGE During 2016, no significant changes were made at BVPS to cause significant negative affect on the environment.

1.4 AQUATIC

MONITORING PROGRAM The 2016 Beaver Valley Power Station (BVPS) Units 1 and 2 Non-Radiological Monitoring Program consisted of an Aquatic Program that included surveillance and field sampling of the Ohio River's aquatic life in the vicinity of the station. The Aquatic Program is an annual program conducted to provide baseline aquatic resources data, to assess the impact of the operation of BVPS on the aquatic ecosystem of the Ohio River, and to monitor for potential impacts ofbiofouling organisms (Corbicula and zebra mussels) on BVPS operations.

This is the 41 st year of operational environmental monitoring for Unit 1 and the 30th year for Unit 2. As in previous years, the results of the program did not indicate any adverse environmental impact to the aquatic life in the Ohio River associated with the operation of BVPS. The results of the 2016 benthic macroinvertebrate survey conducted in May and September indicated that a normal community structure exists in the Ohio River both upstream and downstream of the BVPS. These benthic surveys are a continuation of a Fate and Effects Study conducted from 1990 through 1992 for the P ADEP to assess the ecosystem impacts of molluscicides including Betz Clamtrol CT-1, CT-2, and Nalco H150M that have been used to control biofouling organisms at BVPS. To date, the results of the benthic studies indicate that the use of these biocides have no impact on the benthic community below the BVPS discharge.

Substrate was probably the most important factor influencing the distribution and abundance of the benthic macroinvertebrates in the Ohio River near BVPS. The generally soft muck-type substrate along the shoreline found in 2016 and previous years was conducive to segmented worm ( oligochaete) and midge fly larvae ( chironomid) proliferation.

Increased water clarity due at least in part to the establishment of zebra mussels was noted during 2015. This has increased the amount of submerged aquatic vegetation at stations.

The presence of submerged aquatic vegetation can increase the number of species of macroinvertebrates, especially chironomids (midge flies) that use them as a primary food source and a place to avoid predators.

Sixty-three (63) macroinvertebrate taxa were identified during the 2016 monitoring program (Tables 5.2 and 5.3), which were three more than was identified in 2015. A mean density of 4,360 macroinvertebrates/m 2 was collected in May and 8,387/m 2 in September.

As in previous years, the macroinvertebrate assemblage during 2016 was dominated by burrowing organisms typical 2016 Annual Environmental Report FENOC (BVPS) 2 the macroinvertebrate assemblage during 2016 was dominated by burrowing organisms typical of soft unconsolidated substrates.

In 2016, no new taxa were added to the cumulative list of macroinvertebrates collected near BVPS. Also, no state or Federal threatened or endangered macroinvertebrate species were collected during 2016. In May oligochaetes were the most frequently collected group of macroinvertebrates, while in chironomids dominated.

There were no major differences in the macroinvertebrate community structure between control and non-control stations that could be attributed to operation of BVPS. The overall community structure has changed little since pre-operational years, and program results did not indicate that BVPS operations were affecting the benthic community of the Ohio River. The fish community of the Ohio River near the BVPS was sampled in May (spring), July (summer), September (fall) and November (winter) of 2016 with electrofishing.

Seining could not be completed in 2016, because of extensive erosion at the sampling transects that made conditions unsafe. Since monitoring began in the early 1970's, the number ofidentified fish taxa has increased from 43 to 78 for the New Cumberland Pool. In 2016, 138 fish representing 19 taxa were collected (i.e., handled) during BVPS surveys by electrofishing and seining. This was 297 fewer fish, but only one fewer taxa than collected in 2015. The difference in the number of fish is largely caused by fewer gizzard shad collected in 2016 (15 individuals) compared to 2015 (355 individuals).

All taxa collected in 2016 were previously encountered at BVPS. Benthivores (bottom feeders including suckers and buffalo) and forage species (e.g. gizzard shad and emerald shiners) were generally collected in the highest numbers. The numbers of forage fish were less than those present in 2015, but comparable to the previous two years, due largely to the large number of juvenile gizzard shad collected in 2015. Variations in annual catch were probably attributable to normal fluctuations in the population size of the forage species and the predator populations that rely on them. Forage species, such as gizzard shad, minnow species and shiner species that have high reproductive potentials, frequently respond to changes in natural environmental factors (competition, food availability, cover, and water quality) with large fluctuations in population size. This, in tum, influences their appearance in the sampled populations during annual surveys; Spawning/rearing success due to abiotic factors is usually the determining factor of the size and composition of a fish community.

The annual catch rate in 2016 (0.83 fish per minute) was lower than the previous year (2.56 fish per minute in 2015), but comparable to 2014 (0.86 fish per minute) and 2013 (0.53 fish per minute). The greater electrofishing rate in 2015 was due to the relatively large number of juvenile gizzard shad that were collected in fall and winter. Gizzard shad are schooling fish, so multiple individuals are generally collected when present. They also display high year to year fluctuations in abundance due to spawning success and the extent of over winter mortality.

In 2016, species composition remained comparable among stations.

Common taxa collected in the 2016 surveys included gizzard shad, redhorse sucker species, smallmouth buffalo, carp and smallmouth bass. Little difference in the species composition of the catch and relative composition was observed between the control (1) and non-control stations (2A, 2B and 3). Habitat preference and availability were probably the most important factors affecting where and 2016 Annual Environmental Report FENOC (BVPS) 3 normal community structure for the Ohio River exists near BVPS based on species composition and relative abundance.

In 2016, there was no indication of negative impact to the fish community in the Ohio River from the operation of BVPS. The monthly reservoir ponar samples collected in Unit 1 and 2 cooling towers and the four samples collected at the intake during 2016 indicated that Corbicula were present in the Ohio River and entering the station. In 2016, one settled live and six (6) dead Corbicula were collected from the Unit 1 cooling tower reservoir during monthly reservoir ponar sampling.

In 2016, eight (8) live and seven (7) dead settled Corbicula were collected from the Unit 2 cooling tower reservoir.

The overall low numbers of live Corbicula collected in the sample collected outside the intake and cooling towers in 2016 compared to levels in the 1980's, likely reflects a natural decrease in the density of Corbicula in the Ohio River near BVPS, although the continued presence of Corbicula adults and juveniles near B VPS indicates that they could impact the facility if tire current control program is not continued.

Continued monitoring of Corbicula densities is also recommended to determine whether changes in the Corbicula populations that could impact facility operations are occurring.

In 1995, live zebra mussels were collected for the first time by divers in the BVPS main intake and auxiliary intake structures during scheduled cleanings.

They have been found in the BVPS every year since. Overall, both the number of observations and densities of settled mussels in 2016 were consistent to those recorded in 2008-2015, and much higher than the preceding five years. Although densities of settled mussels are lower than other populations such as the Lower Great Lakes, densities comparable to those in the Ohio River are more than sufficient to cause problems in the operation of untreated cooling water intake systems. Whether the population of zebra mussels in this reach of the Ohio River will remain the same or increase cannot be determined.

In any case, the densities of mussels that presently exist are more than sufficient to impact the BVPS, if continued prudent monitoring and control activities are not conducted.

2.0 ENVIRONMENTAL

PROTECTION PLAN NON-COMPLIANCES There were no Environmental Protection Plan non-compliances identified in 2016. 3.0 CHANGES INVOLVING UNREVIEWED ENVIRONMENTAL QUESTIONS No Unreviewed Environmental Questions were identified in 2016. Therefore, there were no changes involving an Unreviewed Environmental Question.

4.0 NON-ROUTINE ENVIRONMENTAL REPORT 2016 Annual Environmental Report FENOC (BVPS) 4 4.0 NON-ROUTINE ENVIRONMENTAL REPORT There were no non-routine environmental reports in 2016. 2016 Annual Environmental Report FENOC (BVPS) 5

5.0 AQUATIC

MONITORING PROGRAM This section of the report summarizes the Non-Radiological Environmental Program conducted for the BVPS Units 1 and 2; Operating License Numbers DPR-66 and NPF-73. This is a mandatory program, because on February 26, 1980, the NRC granted BVPS's request to delete all of the Aquatic Monitoring Program, with the exception of the fish impingement program (Amendment No. 25), from the Environmental Technical Specifications (ETS). In 1983, BVPS was permitted to also delete the fish impingement studies from the ETS program of required sampling along with non-radiological water quality requirements.

However, in the interest of providing an uninterrupted database, BVPS has continued the Aquatic Monitoring Program. The objectives of the 2016 environmental program were:

• To monitor for any possible environmental impact ofBVPS operation on the benthic macroinvertebrate and fish communities in the Ohio River;

• To evaluate the presence, growth, and reproduction of macrofouling Corbicula (Asiatic clam) and zebra mussels (Dreissena spp.) at BVPS.

• To provide a low level sampling program to continue an uninterrupted environmental database for the Ohio River near BVPS, pre-operational to present; and

• Keep plant operations appraised of any of changes in environmental conditions that may affect the facility.

These objectives have assisted facility personnel in the past. For instance, in the facility's Significant Operating Experience Report (SOER 07-2, October 2008) relative to "Intake Cooling Water Blockage" this Aquatic Monitoring Program was credited as a means of addressing "Changing Environmental Conditions" by looking "for changes in quantity of clam and mussel activity by monitoring the veliger (commonly known as larvae) density in the river and mussel settlement density." 5.1 SITE DESCRIPTION BVPS is located on an approximately 453-acre tract of land on the south bank of the Ohio River in the Borough of Shippingport, Beaver County, Pennsylvania.

The Shippingport Atomic Power Station once shared the site with BVPS before being decommissioned.

Figure 5.1 is a plan view ofBVPS. The site is approximately 1 mile (1.6 km) from Midland, Pennsylvania; 5 miles (8 km) from East Liverpool, Ohio; and 25 miles ( 40 km) from Pittsburgh, Pennsylvania.

The population within a 5-mile (8 km) radius of the plant is approximately 18,000. The Borough of Midland, Pennsylvania has a population of approximately 3,500. The station is situated at Ohio River Mile 34.8 (Latitude:

40° 36' 18"; Longitude:

80° 26' 02") at a location on the New Cumberland Pool that is 3.1 river miles (5.3 km) downstream from Montgomery Lock and Dam and 19.6 miles (31.2 km) upstream from New Cumberland Lock 2016 Annual Environmental Report FENOC (BVPS) 6 and Dam. The Pennsylvania-Ohio-West Virginia border is 5.2 river miles (8.4 km) downstream from the site. The river flow is regulated by a series of dams and reservoirs on the Beaver, Allegheny, Monongahela, and Ohio Rivers and their tributaries.

The study site lies along the Ohio River in a valley, which has a gradual slope that extends from the river at an elevation of 665 ft. (203 m) above mean sea level; to an elevation of 1,160 ft. (354 m) along a ridge south of BVPS. The plant entrance elevation at the station is approximately 735 ft. (224 m) above mean sea level. BVPS Units 1 and 2 have a thermal rating of 2,900 megawatts (MW). Units 1 & 2 have a design electrical rating of 974 MW and 1,009 MW, respectively.

The circulating water systems for each unit are considered a closed cycle system with continuous overflow, using a cooling tower to minimize heat released to the Ohio River. Commercial operation of BVPS Unit I began in 1976 and Unit 2 began operation in 1987. 5.2 STUDY AREA The environmental study area was established to assess potential impacts and consists of four sampling stations, each having a north and south shore (Figure 5 .1 ). Station 1 is located at River Mile (RM) 34.5, approximately

0.3 miles

(0.5 km) upstream of BVPS and is the control station. Station 2A is located approximately

0.5 miles

(0.8 km) downstream of the BVPS discharge structure in the main channel. Station 2B is located in the back channel of Phillis Island; also 0.5 miles downstream of the BVPS discharge structure.

Station 2B is the principal non-control station because the majority of discharges from BVPS Units 1 and 2 are released to this back channel. Station 3 is located approximately two miles (3.2 km) downstream of BVPS and only rarely is influenced by the BVPS discharge.

Significant erosion of Phillis Island has occurred over the past seven years, which has affected sampling Stations 2A and 2B. Also evident at all locations has been the establishment of submerged aquatic vegetation due to increased water clarity, likely caused by the establishment of the zebra mussel (Dreissena polymorpha) in the Ohio River system as well as the reduction of upstream organic runoff and discharges into the nver. 5.3 METHODS CB&I Environmental

& Infrastructure, Incorporated (CB&I) was contracted to perform the 2016 Aquatic Monitoring Program as specified in BVBP-ENV-001-Aquatic Monitoring (procedural guide). This procedural guide references and describes in detail the field and laboratory procedures used in the various monitoring programs, as well as the data analysis and reporting requirements.

These procedures are summarized according to task in the following subsections.

Sampling was conducted according to the schedule presented in Table 5.1. 2016 Annual Environmental Report FENOC (BVPS) 7 5 .3 .1 Benthic Macroinvertebrate Monitoring The benthic macroinvertebrate monitoring program consisted of river bottom sampling using a Ponar grab sampler at four stations on the Ohio River. Prior to 1996, duplicate sampling occurred at Stations 1, 2A, and 3, while triplicate sampling occurred at Station 2B (i.e., one sample at each shoreline and mid-channel) (Figures 5.1 and 5.2). In 1996, a review of the sampling design indicated that sampling should be performed in triplicate at each station to conform to standardized EPA procedures.

Therefore, starting in 1996, triplicate samples were taken at Stations 1, 2A, and 3, as in 1995, with triplicate samples also collected at each shore and mid-channel location at Station 2B. A petite Ponar dredge was used to collect these samples, replacing the standard Ponar dredge used in prior studies. In 2016, benthic macroinvertebrate sampling was conducted as scheduled in May and September.

For each 2016 field effort, 18 benthic samples were collected and processed in the laboratory.

All field procedures and data analyses were conducted in accordance with the procedural guide. The contents of each Ponar grab sample were gently washed in a U.S. Standard No. 30 sieve and the retained contents were placed in a labeled bottle and preserved in ethanol. In the laboratory, rose bengal stain was added to aid in sorting and identifying the benthic organisms.

Macroinvertebrates were sorted from each sample, identified to the lowest taxon practical and counted. Mean density (number/m 2) for each taxon was calculated for each replicate.

Four indices used to describe the benthic community were calculated: Weiner index, evenness (Pielou, 1969), species richness, and the number of taxa. These estimates provide an indication of the relative quality of the macroinvertebrate community.

5.3.2 Fish Monitoring Fish sampling was conducted in 2016 to provide a continuous baseline of data and to detect possible changes that may have occurred in the fish populations in the Ohio River near BVPS. Fish population surveys have been conducted in the Ohio River near BVPS annually from 1970 through 2016. These surveys have resulted in the collection of 73 fish species and five different hybrids. Adult fish surveys were successfully conducted as scheduled in May, July, September, and November 2016. During each survey, fish were scheduled to be sampled at four stations (Stations 1, 2A, 2B and 3) (Figure 5.3). Prior to 2011, all electrofishing was conducted at night. From 2011 to present, due to damage to the onsite boat launch, the crew was required to launch the boat from the Lock 57 Community Park Boat Launch located near Glasgow Pennsylvania.

The launch was only open until one hour after dark, so it was necessary to conduct electrofishing efforts during the day. Electrofishing was completed at all stations and months. Seining was scheduled to be performed at Station 1 (north shore) and Station 2B (south shore of Phillis Island) to sample species that are generally under-represented in electrofishing catches (e.g., young-of-the-year fish and small cyprinids).

In 2016, severe erosion of the shoreline at 2016 Annual Environmental Report FENOC (BVPS) 8 Station 2B and Station 1 made seining unsafe. At each location, there was an immediate off from shore, so there was no place to safely deploy the seine net to collect fish. During each fish sampling effort, conditions at Stations 2B and 1 were reassessed; however, conditions did not improve, so no seine sampling was completed in 2016. In an effort to assess the presence of smaller fish species and juveniles, small fish seen during electrofishing efforts were documented.

Electrofishing was conducted using a boat-mounted electroshocker.

A Smith-Root Type VI A variable voltage, pulsed-DC electrofishing unit powered by a 5-kW generator was used. The voltage selected depended on water conductivity and was adjusted to provide constant amperage (4-6 amps) of the current through the water. The north and south shoreline areas at each station were shocked for at least 10 minutes of unit "on" time (approximately five minutes along each shore) during each survey. When large schools of fish of a single non-game species such as gizzard shad and shiners were encountered during electrofishing efforts, all of the stunned fish were not netted and retrieved onboard the boat. A few fish were netted for verification of identity, and the number of observed stunned fish remaining in the water was estimated.

The size range of the individual fish in the school was also estimated and recorded.

This was done in an effort to expedite sample processing and cover a larger area during the timed electro fishing run. Regardless of the number of individuals, all game fish were boated when observed.

Fish collected during electrofishing efforts were processed according to standardized procedures.

All captured game fishes were identified to species, counted, measured for total length (nearest 1 mm), and weighed (nearest 1 g for fish less than or equal to 1000 g and the nearest 5 g for all other fish). Non-game fishes were counted, and a random subsample of lengths was taken, Live fish were returned to the river immediately after processing was completed.

All fish that were unidentifiable or of questionable identification and were obviously not on the endangered or threatened species list were placed in plastic sample bottles, preserved, labeled and returned to the laboratory for identification.

Any species of fish that had not previously been collected at BVPS was retained for the voucher collection.

Any threatened or endangered species (if collected) would be photographed and released.

5.3.3 Corbicula

Density Determinations for Cooling Tower Reservoirs The Corbicula Monitoring Program at BVPS includes sampling the circulating river water and the service water systems of the BVPS (intake structure and cooling towers). The objectives of the ongoing Monitoring Program were to evaluate the presence of Corbicula at BVPS and to evaluate the potential for and timing of infestation of the BVPS. This program was conducted in conjunction with a program to monitor for the presence of macrofouling zebra mussels (see Section 5.3.5). 2016 Annual Environmental Report FENOC (BVPS) 9 Corbicula enter the BVPS from the Ohio River by passing through the water intakes, and eventually settle in low flow areas including the lower reservoirs of the Units 1 and 2 cooling towers. The density and growth of these Corbicula were monitored by collecting monthly samples from the lower reservoir sidewalls and sediments.

The sampler used on the sidewalls consisted of a D-frame net attached behind a 24-inch long metal scraping edge. This device was connected to a pole long enough to allow the sampler to extend down into the reservoir area from the outside wall of the cooling tower. Sediments were sampled with a petite Ponar dredge. All equipment was tied off prior to sampling to prevent equipment from accidently falling into the reservoirs.

Cooling tower reservoir sampling was historically conducted once per month. Beginning in December 1997, it was decided to forego sampling in cold water months since buildup of Corbicula does not occur then. Monthly sampling has been maintained throughout the warmer water months of the year. In 2016 sampling began in March and ended in November.

In 2016, once each month (March through November), a single petite Ponar grab sample was scheduled to be taken in the reservoir of each cooling tower to obtain density and growth information on Corbicula present in the bottom sediment.

The samples collected from each cooling tower were returned to the laboratory and processed.

Samples were individually washed, and any Corbicula removed and rinsed through a series of stacked U.S. Standard sieves that ranged in mesh size from 1.00 mm to 9.49 mm. Live and dead clams retained in each sieve were counted and the numbers were recorded.

The size distribution data obtained using the sieves reflected clam width, rather than length. Samples containing a small number of Corbicula were not sieved; individuals were measured and placed in their respective size categories.

A scraping sample of about 12 square feet was also collected at each cooling tower during each monthly sampling effort. This sample was processed in a manner consistent with the petite Ponar samples. All samples were successfully collected.

5.3.4 Corbicula

Juvenile Monitoring The Corbicula juvenile study was designed to collect data on Corbicula spawning activities and growth of individuals entering the intake from the Ohio River. From 1988 through 1998, clam cages were deployed in the intake forebay to monitor for Corbicula that entered the BVPS. Observational-based concerns that the clam cages would quickly clog with sediment during high sediment periods and, as a result, would not effectively sample for Corbicula, led to an evaluation of an alternate sampling technique.

From April through June 1997, a study was conducted to compare the results of the clam cage samplers to a petite ponar dredge technique to determine Corbicula presence and density in the BVPS intake bays. It was hypothesized that using a Ponar sampler to collect bottom sediments and analysis of those sediments would provide a more representative sample of Corbicula settlement and growth rates, and had the added benefit of not requiring confined space entry to conduct the sampling.

Results of the study confirmed this hypothesis.

2016 Annual Environmental Report FENOC (BVPS) 10 During the 1998 sanwling season, at the request of BVPS personnel, all clam cages were removed after the May collection.

Monthly petite ponar grabs from the forebay in the intake building continued thereafter.

Samples were processed in the same manner as Cooling Tower samples (Section 5.3.3). From 2002 to present, because of site access restrictions, sampling with the petite ponar has been moved to the Ohio River directly in front of the Intake Structure Building.

Collections are presently scheduled to be made in conjunction with the fisheries sampling (May, July, September, and November).

During each sampling month two Ponar grabs are taken approximately 20 feet offshore of the intake building.

These grab samples are processed in the same manner as when they were collected from within the Intake Structure Building.

5.3.5 Zebra

Mussel Monitoring The Zebra Mussel Monitoring Program includes sampling the Ohio River and the circulating river water system of the BVPS. The objectives of the Monitoring Program were: (1) To identify if zebra mussels were in the Ohio River adjacent to BVPS and provide early warning to operations personnel as to their possible infestation; (2) To provide data as to when the larvae were mobile in the Ohio River and insights as to their vulnerability to potential treatments; and (3) To provide data on their overall density and growth rates under different water temperatures and provide estimates on the time it requires these mussels to reach the size and density that could impact the plant. The zebra mussel sampling for settled adults was historically conducted once per month throughout the year. Beginning in December 1997, it was decided to forego sampling in the colder water months of each year, since buildup of zebra mussels and growth of the individuals that were present, does not occur. Monthly sampling has been maintained throughout the balance of the year. In 2016 sampling occurred from March through November.

A pump sample for zebra mussel veligers was collected at the barge slip location monthly from , April through October in 1996 and 1997. The scope of the sampling was expanded in 1998 to also include the intake structure.

In June 1998, the Emergency Outfall and Emergency Outfall Impact Basin locations were also added. Additional pump samples were collected from the cooling towers of Unit 1 and Unit 2 in October 1998. In 2016, veliger sampling began in April and was conducted monthly through October. At the Intake Structure and Barge Slip the following surveillance techniques were used:

• Wall scraper sample collections on a monthly basis (March through November) from the barge slip and the riprap near the intake structure to detect attached adults; and 2016 Annual Environmental Report FENOC (BVPS) 11

• Pump sample collections from the barge slip and outside the intake structure, to detect the plank.tonic early life forms (April through October).

At each of the cooling towers the following techniques were used:

• Monthly reservoir scraper sample collections in each cooling tower (March through November);

and

• Pump samples in April through October to detect plank.tonic life forms. At the Emergency Outfall and the Splash Pool the following techniques were used:

• Monthly scraper sample collections in each (March through November);

and

• Pump samples in each from April through October to detect plank.tonic life forms. 5.3.6 Reports Each month, activity reports that summarized the activities that took place the previous month were prepared and submitted.

These reports included the results of the monthly Corbicula/zebra mussel monitoring including any trends observed and any preliminary results available from the benthic and fisheries programs. The reports addressed progress made on each task, and reported any observed biological activity of interest.

5.4 RESULTS

OF THE AQUATIC MONITORING PROGRAM The following sections summarize the findings for each of the program elements.

Sampling dates for each of the program elements are presented in Table 5.1. 5 .4.1 Benthic Macroinvertebrate Monitoring Program Benthic surveys were performed in May and in September 2016. Benthic samples were successfully collected using a petite ponar grab sampler at Stations 1, 2A, 2B, and 3 (Figure 5.2). Triplicate samples were taken off the south shore at Stations 1, 2A, and 3. Sampling at Station 2B, in the back channel of Phillis Island, consisted of triplicate petite Ponar grabs at the south side, middle, and north side of the channel (i.e., Sample Stations 2B 1, 2B2, and 2B3, respectively).

Substrate type is an important factor in determining the composition of the benthic community.

2016 Annual Environmental Report FENOC (BVPS) 12 1 I 1 I I The habitats in the vicinity of BVPS are the result of damming, channelization, and river traffic. Shoreline habitats at the majority of sampling locations were generally in depositional areas that consisted of soft substrates composed of mixes of sand, silt, and detritus.

One exception was along the north shoreline of Phillis Island at Station 2A where hard-pan clay overlain with a thin layer of fine sand dominated.

The other distinct habitat, hard substrate (gravel and cobble), was located in mid-channel in the back channel of Phillis Island. The hard substrate was probably the result of channelization and ongoing scouring by river currents.

In general, the substrates found at each sampling location have been consistent from year to year. Increased water clarity due at least in part to the establishment of zebra mussels was noted during 2016. This is a continuation of a trend noted over the past three years. This has greatly increased the amount of submerged aquatic vegetation at all of the stations.

The presence of submerged aquatic vegetation can increase the number of species of macroinvertebrates, especially chironomids (midge flies) and gastropods (snails) that use them as a primary food source. Also zebra mussel filtering moves much of the available nutrients from the water column to the bottom, which also can affect the type and density of macroinvertebrates present in the project area. Sixty-three (63) macroinvertebrate taxa were identified during the 2016 monitoring program (Tables 5.2 and 5.3), which were three more than was identified in 2015. A mean density of 4,360 macroinvertebrates/m 2 was collected in May and 8,387/m 2 in September (Table 5.4). As in previous years, the macroinvertebrate assemblage during 2016 was dominated by burrowing organisms typical of soft unconsolidated substrates.

Oligochaetes (segmented worms), mollusks (clams and snails) and chironomid (midge fly) larvae were abundant (Table 5.4). Eighteen (18) taxa of chironomids and 19 taxa of oligochaetes were collected.

This is the same number of chironomid taxa and one fewer oligochaete taxa than collected in 2015. Twelve (12) taxa of mollusks were collected in 2016; one more than in 2015. As was the case in 2015, the total mean density of organisms was higher in September than in May. Thirty-six (36) taxa were present in the May 2016 samples. Fifty-two (52) taxa were present in the September samples (Table 5.3.1 and 5.3.2). Twenty-five (25) of the 63 taxa were present in both May and September.

As in 2015, immature tubificid worms were numerically the most abundant organism in May 2016. However, the most abundant species in September 2016 was the zebra mussel (Dreissena polymorpha), while immature tubificid worms were the most abundant organism in September 2015. The macrofouling Asiatic clam (Corbicula) has been observed in the Ohio River near BVPS from 1974 to present. Macrofouling zebra mussels were first collected in the BVPS benthic samples in 1998. Adult zebra mussels, however, were detected in 1995 and 1996 by divers in the BVPS main and auxiliary intake structures during scheduled cleaning operations.

Zebra mussel veligers, adults and juveniles were collected during the 1997-2016 sampling programs (see Sections 5.4.5 Zebra Mussel Monitoring Program).

Both live adult Corbicula and adult zebra mussels were collected in benthic macroinvertebrate samples in 2016. Corbicula and zebra mussels were collected in both May and September samples. Zebra mussels were the second most abundant taxa collected in the May samples and the most abundant in September.

The zebra mussels collected in September were mostly mussels that had settled earlier in 2016, which 2016 Annual Environmental Report FENOC (BVPS) 13 indicates higher than usual recruitment in 2016. This may increase the potential for fouling by zebra mussels at BVPS next year as these individuals grow and reproduce.

No new taxa of macroinvertebrates were collected near BVPS in 2016 (Table 5.2). Also no state or Federal threatened or endangered macroinvertebrate species were collected during 2016. In the May 2016 samples, oligochaetes accounted for the highest mean density of macroinvertebrates (3,220/m 2 or 74 percent of the total density) (Table 5.4). Oligochaetes also were the dominant taxon in May 2015. Mollusks were the second most abundant species in May (569/m 2 or 13 percent of the total density).

Chironomids (511/m 2 or 12 percent of the total) and organisms other than oligochaetes, chironomids and mollusks ("others")

(60/m 2 or one percent) were both present in May. In September 2016 samples, chironomids accounted for the highest mean density of macroinvertebrates (3,423/m 2 or 41 percent of the total density) (Table 5.4). Mollusks had the next highest mean density in September 2016 (3,139/m 2 or 37 percent of the total density), followed by oligochaetes (1,209/m 2 or 14 percent) and the "others" category (616/m 2 or seven percent).

In May 2016, the highest density of macroinvertebrates (11,022/m 2) occurred at Station 1. Oligochaetes were over three times as abundant at Station 1 as at any other location.

In September, the highest densities of macroinvertebrates occurred at Station 2B 1 (14,462/m 2) and Station 2B3 (14,920/m 2). At Station 2B 1 this was due to a high density of mollusks, principally zebra mussels that were collected (10,449/m 2) at this location.

). At Station 2B3 this was due to a high density of chironomids that were collected (9,904/m 2) at this location.

In May the lowest mean density of organisms was 817/m 2 , which occurred at Station 2A. In September, the lowest mean density of organisms occurred at Station 1 (2,93 8/m 2). For a comparison of the control to non-control stations, Station 1 was designated the control station, because it is always out of the influence of the BVPS discharge and Station 2B (mean density of Station 2B 1, 2B2, and 2B3) was designated as the non-control station, since it is the station most regularly subjected to BVPS's discharge.

Stations 3 and 2A may be under the influence of the plume under certain conditions, but it is unlikely that they are regularly influenced by BVPS. The mean density of macroinvertebrates in the control station was almost three times higher (11,022/m 2) than that of the non-control station (3,827/m 2) in May (Table 5.5). The much higher densities of oligochaetes at the control station contributed to the majority of this difference.

The reverse was true in September, when the density of macroinvertebrates present at the control station (10,869/m 2) was approximately over 3 .5 times higher than at the control station (2,924/m 2). Relatively higher densities of all categories over organisms occurred at the control station. Differences in both months were within the expected range of variation for natural populations of macroinvertebrates and likely not due to any impact of plant operation.

Indices that describe the relative diversity, evenness, and richness of the macroinvertebrate 2016 Annual Environmental Report FENOC (BVPS) 14 population structure among stations and between control and non-control sites were calculated.

A higher Shannon-Weiner diversity index indicates a relatively better structured assemblage of organisms, while a lower index generally indicates a lower quality or stressed community.

Evenness is an index that estimates the relative contribution of each taxon to the community assemblage; the closer to 1.00, the healthier the community.

The community richness is another estimate of the quality of the macroinvertebrate community with a higher richness number indicating a healthier community.

The Shannon-Weiner diversity indices in May 2016 collections ranged from 0.15 at Station 1 to 0.79 at Stations 2A (Table 5.6). In May, evenness ranged from 0.15 at Station 1 to 0.87 at Station 2A. Richness was greatest at Station 2B2 (2.73) and lowest at Station 1 (0.75). In general the indices were lower in May 2016 than in 2015. This was due in part to the relatively higher densities of immature tubificids present in 2016 and the lower density of tubificids that were mature enough to identify.

In 2016, 90 percent of the individual oligochaetes collected in May could only be identified as immature tubificids while in 2015 only 30 percent of the oligochaetes collected in May were identified as immature tubificids.

This also resulted in a greater number oftaxa identified in 2015. This difference in maturation rate of oligochaetes was likely due to natural annual differences and not related to any effect of plant operations.

The Shannon-Weiner diversity of the macroinvertebrate community (0.72 to 1.21), evenness (0.52 to 0.79) and richness (2.77 to 4.65) in September 2016 were generally higher than in May. There was also an increase in the number of taxa present at each station in September compared to that station in May. Relatively higher numbers of taxa are frequently present in early fall due to the increased numbers of aquatic stages of insects, especially chironomids, as well as the ability to identify many of the tubificids that are lumped together as immature in May to lower taxonomic levels in September.

A comparable increase in index values in September compared to May was also observed in each year from 2010 through 2015. In May 2016, the number of taxa was lower in the control station (Station 1) than in the control stations (2Bl, 2B2, 2B3) (11 in the control versus 20, 16 and 16 in the non-controls).

The diversity, evenness and richness indices were also lower at the control station than the controls (Table 5.6). In September 2016 the number of taxa at the control stations were also lower the control station (Station 1) than in the non-control stations (2Bl, 2B2, 2B3) (18 in the control versus 35, 24 and 34 in the non-controls).

The indices in the control station were, however, in general comparable to those in the non-control stations.

Similar trends were apparent in the previous six study years and were likely due to natural variations in the local populations at these locations.

No impacts of the BVPS on the benthic community, as measured by differences between control and non-control zones, were evident in either May or September.

Substrate was probably the most important factor controlling the distribution and abundance of the benthic macroinvertebrates in the Ohio River near BVPS. Soft substrates that generally existed along the shoreline are conducive to oligochaete, chironomid, and mollusk habitation and limit species of macroinvertebrates that require a more stable bottom. There was an apparent change in the chironomid community throughout the study zone, as evidenced by their increased densities and the type of species present. This is likely due to an increase in the submerged vegetation noted along the shoreline.

Vegetation provides an ideal habitat for many chironomid 2016 Annual Environmental Report FENOC (BVPS) 15

---

---species that use it for grazing on the surficial phytoplankton and a place to avoid predators.

This change in the chironomid community is not caused by BVPS operations.

The density of macroinvertebrates in May and September 2016 were the highest since 2005 but fell within the range of densities of macroinvertebrates colleeted at BVPS in previous years (Table 5.7). Although the species of macroinvertebrates and their relative densities may have increased slightly due to increased light penetration caused by the increased density of zebra mussels and the subsequent increase in submerged aquatic vegetation in the nearshore area, the overall macroinvertebrate community structure has changed little since pre-operational years. Any changes have occurred at both control and non-control sites, so the available evidence does not indicate that B VPS operations have affected the benthic community of the Ohio River. 5.4.2 Fish Sampling Program In 2016, 138 fish representing 19 taxa were collected (i.e. handled) during BVPS surveys by electrofishing and seining (Table 5.8). This was 297 fewer fish and only one fewer taxa than collected in 2015. The difference in the number offish was largely caused by fewer gizzard shad collected in 2016 (15 individuals) compared to 2015 (355 individuals).

All taxa collected in 2016 were previously encountered at BVPS. Further, in 2016, severe erosion of the shoreline at Station 2B and Station 1 made seining unsafe. At each location, there was an immediate drop-off from shore, so there was no place to safely deploy the seine net to collect fish. During each r fish sampling effort, conditions at Stations 2B and 1 were reassessed; however, conditions did not improve, so no seine sampling was completed in 2016. The most common species in the 2016 BVPS surveys that were collected by electrofishing were smallmouth buffalo (20.3% of the total catch), followed by golden redhorse sucker (15.2%), carp (13.8%), gizzard shad (10.9%), shorthead redhorse sucker (6.5%) and pumpkinseed sunfish (5.1 %). None of the remaining 13 species contributed to more than five percent of the total handled catch. The most frequently observed, but not handled fish in 2016 were emerald shiners (Table 5.15). Game fish collected in 2016 included channel catfish, bluegill, smallmouth bass, walleye, spotted bass, pumpkinseed, black crappie, largemouth bass, sauger, and yellow perch. Game fish represented 24.6% of the total handled catch. The number of fish collected in 2016 was somewhat lower than the total number collected in 2011 (151 fish), the last time electrofishing was conducted at night. The number of species collected in 2016 was also fewer than in 2011 when 22 species were encountered.

In general electrofishing at night has been demonstrated to be more productive than during the day in riverine systems. Movements of many species of fish into shallower water at night to feed, makes them more susceptible to the electrofishing technique.

A total of 25 fish representing 10 species was captured during the May (spring) 2016 sampling event (Table 5.10). Gizzard shad was the most abundant species and represented 40.0% of the electrofishing catch, followed in abundance by shorthead redhorse sucker (representing 20.0% of 2016 Annual Environmental Report FENOC (BVPS) 16 the total catch), bluegill (8.0%), and golden redhorse sucker (8.0%). No other species contributed to more than five (5) percent of the May electrofishing catch. Bluegill, smallmouth bass, walleye and pumpkinseed sunfish were the game species collected in May. Seining was not conducted due to unsafe conditions.

A total of 13 fish representing five (5) species was captured during the July (summer) 2016 sampling event (Table 5 .11 ). Smallmouth buffalo and longnose gar were the most abundant species and represented 61.5% and 15.4% of the catch, respectively.

Every other fish species collected was represented by a single individual.

Channel catfish were the only game species collected in July. Seining was not conducted due to unsafe conditions.

During the September (fall) 2016 sampling event, 36 fish representing eight (8) taxa were collected (Table 5 .12). All of the fish were collected during electro fishing efforts. Gizzard shad were the most abundant species and contributed to 58.3 percent on the total. Longnose gar (11.1% of the total), smallmouth buffalo (11.1%), golden redhorse sucker (5.6%), and smallmouth bass (5.6%) were also collected.

None of the other species contributed to greater than five percent of the catch. Bluegill and smallmouth bass were the only game species collected in September.

Seining was not conducted due to unsafe conditions.

During the November (winter) 2016 sampling event, 64 fish representing 16 taxa were collected (Table 5 .13 ). Cooler near shore water temperatures likely contributed to more individuals and species collected in November than in the other sampled months. Carp and smallmouth buffalo were the most abundant species collected by electrofishing and contributed to 21.9% and 20.3% of the total catch, respectively.

Gizzard shad (7.8% of the total), golden redhorse sucker (7.8%), smallmouth bass (6.3%), and yellow perch (6.3%) were the only other species that contributed to greater than five percent of the catch. Game species collected in November included black crappie, largemouth bass, pumpkinseed sunfish, sauger, smallmouth bass, spotted bass, and yellow perch. Seining was not conducted due to unsafe conditions.

Electrofishing catch rates are presented in Tables 5.15, 5.16, 5.17, and 5.18 for fish that were boated and handled during the 2013 through 2016 surveys by season (FENOC 2014, 2015 and 2016). In 2016, the annual catch rate was 0.83 fish per minute. In 2016, the greatest seasonal catch rate occurred in winter (November) when the catch rate was 1.56 fish per minute. The lowest catch rate occurred in summer (July) with a rate of 0.32 fish per electrofishing minute. The annual catch rate in 2016 (0.83 fish per minute) was lower than the previous year (2.56 fish per minute in 2015), but comparable to 2014 (0.86 fish per minute) and 2013 (0.53 fish per minute). The greater electrofishing rate in 2015 was due to the relatively large number of juvenile gizzard shad thatwere collected in fall and winter. Gizzard shad are schooling fish, so multiple individuals are generally collected when present. They also display high year to year fluctuations in abundance due to spawning success and the extent of over winter mortality.

The gizzard shad collected in fall and winter 2015 likely were spawned in spring and early summer 2015 and became large enough to be collected by electrofishing.

The gizzard shad collected during 2016 were generally smaller adults and probably individuals that were probably spawned in 2015. 2016 Annual Environmental Report FENOC (BVPS) 17 In each year, the lowest catch rate occurred in summer, when fish generally move to deeper water where electrofishing is ineffective to avoid warm inshore water temperatures.

Over the four years, the highest seasonal catch rate occurred in fall 2015 (7 .11 fish per minute), which was due to the large number of juvenile gizzard shad present. The results of the electrofishing sampling effort in 2016 (Table 5.9) did not indicate any major differences in species composition between the control station (1) and the non-control Stations 2A, 2B, and 3. In both, smallmouth buffalo, carp and golden redhorse sucker were the most abundant species. A greater number of fish representing more species was captured at control stations than control station. This was most likely due to the extra effort expended at non-control stations versus control station. There are three non-control stations and only one control station so there was three times the effort at the non-control stations.

In 2016, there were just under three times as many fish collected at the non-control stations than at the control station with three times the effort. In 2016, species composition remained comparable among stations.

Common taxa collected during the 2016 surveys included gizzard shad, redhorse sucker species, smallmouth buffalo, carp and smallmouth bass. Little difference in the species composition of the catch and relative composition was observed between the control (1) and non-control stations (2A, 2B and 3). Habitat preference and availability were probably the most important factors affecting where and when different species of fish are collected.

The results of the 2016 fish surveys indicated that there is a normal community structure in the Ohio River in the vicinity of BVPS based on species composition and relative abundance of fish observed during the surveys. Benthivores (bottom feeders including suckers and buffalo) and forage species (e.g. gizzard shad and emerald shiners) were generally collected in the highest numbers. The number of forage fish was less than in 2015, but comparable to the previous two years, due largely to the large number of juvenile gizzard shad collected in 2015. Variations in annual catch were probably attributable to normal fluctuations in the population size of the forage species and the predator populations that rely on them. Forage species, such as gizzard shad, minnow species and shiner species that have high reproductive potentials, frequently respond to changes in natural environmental factors (competition, food availability, cover, and water quality) with large fluctuations in population size. This, in turn, influences their appearance in the sampled populations during annual surveys. Spawning/rearing success due to abiotic factors is usually the determining factor of the size and composition of a fish community.

In addition, differences in electrofishing catch rate can be attributed to environmental conditions that prevail during sampling efforts. High water, increased turbidity, waves, and swift currents that occur during electrofishing efforts in some years can affect the collection efficiency in any given month. In 2016, as in the previous three years, continued increased water clarity was apparent during all months sampled. A direct result of the increased clarity was the abundance of rooted submerged aquatic vegetation throughout the study reach. The amount of rooted vegetation in 2016 was much greater than in any other year sampled. This increase in vegetation is likely the result of an increased photic zone due to zebra mussels filtering organic and inorganic particulates from the water and redistributing them to the benthic layer. The presence of rooted vegetation and increased water clarity can change the distribution of many of the fish 2016 Annual Environmental Report FENOC (BVPS) 18 species present in the study reach. This may have contributed to the increased presence of sight feeding species such as black crappie, bluegill, pumpkinseed, largemouth bass, smallmouth bass, spotted bass and yellow perch in the study area. Results from the 2016 fish surveys indicated that a normal community structure for the Ohio River exists near BVPS based on species composition and relative abundance.

In 2016, there was no indication of negative impact to the ftsh community in the Ohio River from the operation of BVPS. 5.4.3 Corbicula Monitoring Program In 2016, one settled live Corbicula was collected from the Unit 1 cooling tower reservoir during monthly reservoir ponar sampling (Table 5.19 and Figure 5.5). It was collected in March and was between 2.00 mm to 3.4 mm, which indicated that it had settled late in 2015. Six (6) dead Corbicula that were between 2.00 mm and greater than 9.5 mm were also collected.

The seasonal average density of settled live Corbicula was 5/m 2 , which was about 10 percent of the density of Corbicula in the Unit 1 cooling tower in 2015, but comparable to the density in 2014. Dead Corbicula were collected in May, July and September and were probably killed by scheduled molluscicide treatments.

No Corbicula were collected in the scraping samples. Corbicula juveniles were also collected in monthly pump samples collected in the Unit 1 cooling tower reservoir in July, August, and September.

In 2016, eight (8) live settled Corbicula were collected from the Unit 2 cooling tower reservoir (Table 5.20 and Figure 5.6). Live mussels were collected in April, May, and November. They were between 2.00 mm to 9.49 mm in size, which indicates that some settled prior to 2016. Seven dead Corbicula were also collected during 2016. These were between 2.00 mm to greater than 9.50 mm and likely represented a number of year classes. The dead Corbicula were collected in May, July, September and November and were probably killed by scheduled molluscicide treatments.

The season average density of settled live Corbicula was 38/m 2 that was slightly lower than in 2015, but comparable to 2014. The highest density of settled Corbicula occurred in November when a density of 172 Corbiculalm 2 was present. No Corbicula were collected in the scraping samples. Corbicula juveniles were also collected in monthly pump samples collected in the Unit 2 cooling tower reservoir in July, August, and September.

Corbicula juveniles were collected at non cooling tower locations during monthly pump sampling in 2016. Corbicula juveniles were collected each month from May through September at the Emergency Outfall Facility and the splash pool. They were collected from June through September at the barge slip and from July through September at the Ohio River intake sample. Densities of Corbicula juveniles exceeded 1,500 individuals per cubic meter in the July Emergency Outfall Facility and the splash pool. This indicates a significant population of Corbicula in the vicinity of the BVPS that could impact plant operations if steps were not taken to control the mussels. 2016 Annual Environmental Report FENOC (BVPS) 19 In 2016, BVPS continued its Corbicula control program, which included the use of a molluscicide to prevent the proliferation of Corbicula within BVPS. BVPS was granted permission by the PADEP to use a molluscicide to target the Unit 1 river water system and the Unit 2 service water system. In 1990 through 1993, the molluscicide applications focused on reducing the Corbicula population throughout the entire river water system of each BVPS plant (Units 1 and 2). In 1994 and 1995, the applications targeted the internal water systems; therefore, the molluscicide concentrations in the cooling towers were reduced during applications.

Consequently, adult and juvenile Corbicula in the cooling towers often survived the applications.

Reservoir sediment samples taken after molluscicide applications represent mortality of Corbicula in the cooling tower only and do not reflect mortality in BVPS internal water systems. The monthly reservoir sediment samples and pump samples collected in Units 1 and 2 Cooling Towers in recent years demonstrated that Corbicula were entering and colonizing the reservoirs.

Only 9 live and 13 dead settled Corbicula were collected in the cooling towers in 2016; however, their presence in the cooling tower pump . samples indicates that they still are available for establishment in the cooling towers. The recent decrease of Corbicula at the BVPS returns densities to levels more consistent with densities in the Ohio River in the mid-1990's, but well below those present during the 1980's. Whether the relatively low density of Corbicula in 2016 is indicative of permanent lower levels in the environment or due to natural variability is uncertain, however, continued monitoring of Corbicula densities is recommended.

5.4.4 Corbicula

Juvenile Monitoring Program Figure 5.7 presents the abundance and size distribution data for samples collected in the Ohio River near the intake structure by petite ponar dredge in 2016. Eighteen (18) live individuals were collected 2016 compared to 60 in 2015 and 17 in 2014. They were collected in every month sampled. In 2016, they ranged in size from the 2.00 mm to 3.34 mm size range that were spawned in late 2015 to greater than 9.50 mm that were spawned in prior years. A summer spawning period typically occurs in the Ohio River near BVPS each year when preferred spawning temperatures (60-65° F) are reached (Figure 5.8). The offspring from this spawning event generally begin appearing in the sample collections in June. The settled clams then generally increase in size throughout the year. The number of individuals collected in 2016 was three to five times less than 2015 but comparable to each of the previous four years. This is most likely due to normal variability in the population in the Ohio River. In any case the densities of Corbicula continue to be low relative what was present in the 1980's. The overall low numbers of live Corbicula collected in the sample collected outside the intake and cooling towers in 2016, compared to levels in the J 980's, likely reflects a natural decrease in the density of Corbicu/a in the Ohio River near BVPS, although the continued presence of Corbicula adults and juveniles near BVPS indicates that they could impact the facility if the current control program is not continued.

Continued monitoring of Corbicula densities is also recommended to determine whether changes in the Corbicula populations that could impact facility operations were occurring.

2016 Annual Environmental Report FENOC (BVPS) 20

5.4.5 Zebra

Mussel Monitoring Program Zebra mussels (Dreissena polymorpha) are exotic freshwater mollusks that have ventrally flattened shells generally marked with alternating dark and lighter bands. They are believed to have been introduced into North America through the ballast water of ocean-going cargo vessels probably from Eastern Europe. They were first identified in Lake St. Clair in 1988 and rapidly spread to other Great Lakes and the Mississippi River drainage system, and have become increasingly abundant in the lower, middle, and upper Ohio River. They use strong adhesive byssal threads, collectively referred to as their byssus, to attach themselves to any hard surfaces (e.g., intake pipes, cooling water intake systems, and other mussels).

Responding to NRC Notice No. 89-76 (Biofouling Agent-Zebra Mussel, November 21, 1989), BVPS instituted a Zebra Mussel Monitoring Program in January 1990. Studies have been conducted each year since then. Zebra mussels were detected in both the pump samples (Figures 5.9 and 5.10) and the substrate samples (Figure 5 .11 and 5 .12) in 2016. Veligers were collected at all of the six sites that were sampled in 2016. Zebra mussels were collected in veliger pump samples from May through October 2016 after not being collected in April. The lack of veligers in April can be attributed to the colder than usual late winter and early spring that occurred in 2016. Spawning begins as water temperature reach approximately 14° C and peaks at water temperatures of 21° C. Veliger densities usually peak about two weeks after the optimum water temperature for spawning is reached. Veliger densities then fall off as veligers mature and settle, although female mussels broadcast mature eggs throughout the season. River water temperature in April was 15° C, which is just above the spawning threshold.

Veligers were present at all sampled sites from May through October. A peak in zebra mussel veligers occurred at most sampled locations in July. The majority of these veligers were D-form and very recently spawned and not able to settle. The percentage of mussels capable of settling increased though the rest of the sampling season. The density of mussel veligers present in August were also high. They were much lower at all locations, in September, yet were still present at all locations.

In October, veligers were not found at any location except the splash pool where they were present in very low densities (10/m 3). This indicated that the majority of mussel had settled and no more veligers were being produced.

This is supported by a river water temperature rapidly decreasing between the September and October sampling events. The greatest density of veligers was present in the sample collected from the splash pool in July (136,250/m 3). This was 1.7 times greater than the next highest veliger density of the peak density ofveligers in the past five years, and the highest density recorded since 2010. In 2016, settled zebra mussels were collected only in scrape samples at the splash pool, the barge slip and the intake structure (Figures 5.11 and 5.12). Those in the splash pool were collected in low densities in March. The highest density of settled mussels in any sample collected was at the barge slip (35.9 mussels/m 2) in March. The mussels collected at the barge slip and intake structure included individuals that were capable of reproducing.

The density of collected adult zebra mussels in 2016 was somewhat higher than the densities that occurred in 2015 and 2014. Overall, both the number of observations and densities of settled mussels in 2016 were consistent to those recorded in 2008-2015, and much higher than the preceding 5 years. Although densities 2016 Annual Environmental Report FENOC (BVPS) 21 r---of settled mussels are low compared to other populations such as the Lower Great Lakes, densities comparable to those in the Ohio River are sufficient to cause problems in the operation of untreated cooling water intake systems. Whether the population of zebra mussels in this reach of the Ohio River will remain the same or increase cannot be determined.

In any case, the densities of mussels that presently exist are more than sufficient to impact the BVPS, if continued prudent monitoring and control activities are not conducted.

2016 Annual Environmental Report FENOC (BVPS) 22

6.0 ZEBRA

MUSSEL AND CORBICULA CONTROL ACTIVITIES In 2016, BVPS continued its Corbicula and zebra mussel control program (27th year), which includes the use of a molluscicide to prevent the proliferation of Corbicula and zebra mussels within BVPS. BVPS was granted permission by the P ADEP to use a molluscicide to target the Unit 1 river water system and the Unit 2 service water system. In 1990 through 1993, the molluscicide applications (CT-1) focused on reducing the Corbicula population throughout the entire river water system of each BVPS plant (Units 1 and 2). In 1994 through 2006, the CT-1 or CT-2 (reformulated CT-1) applications targeted zebra mussels and Corbicula in the internal water systems; therefore the molluscicide concentrations in the cooling towers were reduced during CT-1 or CT-2 applications.

Consequently, adult and juvenile Corbicula in the cooling towers often survived the applications.

Reservoir sediment samples taken after CT-1 or CT-2 applications represented mortality of Corbicula in the cooling tower only and do not reflect mortality in BVPS internal water systems. In 2007 BVPS began using Nalco H150M as the molluscicide.

This product, which has the same active ingredients as the CT-2 and CT-2, was applied in the same manner. In addition to clamicide treatments, preventive measures were taken that included quarterly cleaning of the Intake Bays. The bay cleanings are intended to minimize the accumulation and growth of mussels within the bays. This practice prevents creating an uncontrolled internal colonization habitat. 2016 Annual Environmental Report FENOC (BVPS) 23 --------------------------------------------

7.0 REFERENCES

Commonwealth of Pennsylvania, 1994. Pennsylvania's Endangered Fishes, Reptiles and Amphibians.

Published by the Pennsylvania Fish Commission.

Counts, C. C. III, 1985. Distribution of Corbicula jluminea at Nuclear Facilities.

Division of Engineering, U.S. Nuclear Regulatory Commission.

NUREGLCR.

4233. 79 pp. Dahlberg, M. D. and E. P. Odum, 1970. Annual cycles of species occurrence, abundance and diversity in Georgia estuarine fish populations.

Am. Midl. Nat. 83:382-392.

FENOC, 2003. Annual Environmental Operating Report, Non-radiological.

First Energy Nuclear Operating Company, Beaver Valley Power Station, Unit No. 1 & 2. 113 pp. FENOC, 2004. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 82 pp. FENOC, 2005. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 82 pp. FENOC, 2006. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 82 pp. FENOC, 2007. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 82 pp. FENOC, 2008. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 82 pp. FENOC, 2009. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 82 pp. FENOC, 2010. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 82 pp. FENOC, 2011. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 82 pp FENOC, 2012. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 82 pp FENOC, 2013. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 82 pp FENOC, 2014. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1 &2. 82 pp 2016 Annual Environmental Report FENOC (BVPS) 24 I I , ' I I i I I I . -' ' ! ' FENOC, 2015. Annual Environmental Operating Report, Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 83 pp FENOC, 2016. Annual Environmental Operating Report Non-radiological.

First Energy Operating Company, Beaver Valley Power Station, Unit No. 1&2. 83 pp Hutchinson, G. E., 1967. A treatise on limnology.

Vol. 2, Introduction to lake biology and the limnoplankton.

John Wiley and Sons, Inc., New York. 1115 pp. Hynes, H.B. N., 1970. The ecology of running waters. Univ. Toronto Press, Toronto. NRC, IE Bulletin 81-03: Flow Blockage of Cooling Tower to Safety System Components by Corbicula sp. (Asiatic Clam) and Mytilus sp. (Mussel).

Pielou, E. C., 1969. An introduction to mathematical ecology. Wiley Interscience, Wiley & Sons, New York, NY. Robins, C. R., R. M. Bailey, C. E. Bond, J. R. Brooker, E. A. Lachner, R. N. Lea, and W. B. Scott, 1991. Common and Scientific Names of Fishes from the United States and Canada (fifth edition).

Fisheries Society Special Publication No. 20: 1-183. Shiffer, C., 1990. Identification Guide to Pennsylvania Fishes. Pennsylvania Fish Commission, Bureau of Education and Information.

51 pp. Winner, J.M., 1975. Zooplankton.

In: B. A. Whitton, ed. River ecology. Univ. Calif. Press, Berkeley and Los Angeles. 155-169 pp. 2016 Annual Environmental Report FENOC (BVPS) 25 2016 Annual Environmental Report FENOC (BVPS) 8.0 TABLES 26 TABLE5.1 BEA VER VALLEY POWER STATION (BVPS) SAMPLING DATES FOR 2016 Study Jan Feb Mar Apr May Jllll Jul . *Aug Beirthic Macroinvertebrate:

.. Fi<;h Corbicula and Zebra, Mussel Zebra Mussel Veliger ",* 2016 Annual Environmental Report FENOC (BVPS) 30 28 28 31 31 27 31 30 27 25 31 30 27 25 27 Sep Oct. Nov Dec 13 13 2 13 27 2 13 27 Table 5.2 Systematic List of Macroinvertebrates Collected From 1973 Through 2016 in The Ohio River Near BVPS Phylum Class Family Sub-Famil Porifera Cnidaria Hvdrozoa Clavidae Hvdridae Platyhelminthes Tricladida Rhabdocoela Nemertea Nematoda Entoprocta Ectoorocta Annelida Olie:ochaeta Aeolosomatidae Enchytraeidae Naididae 2016 Annual Environmental Report FENOC (BVPS) Genus andSpecies Spongilla.fragi/is Cordy/01Jhora lacustris Crasf)edacusta sowerbii Hydra sp. Urnatella J<raci/is Fredericella sp. Paludicella articulata Pectinatella sp. Plumatella sp. A/Iona is 1Jectinata Amphichaeta leydigi Amphichaeta sp. Arcteonais lomondi Au/ophorus sp. ChaetoJ<aster dia1Jhanus C. diastrophus Dero dif!itata Deroflabel/iger D. nivea Dero sp. Na is barbata N. behningi N. bretscheri N. communis N. elinguis N. pardalis N. 1Jseudobtusa N. simplex N. variabi/is Nais sp. 01Jhidonais sementina Paranais frici Paranais /itoralis Paranais sp. PiJ<uetiella michif!anensis Pristina idrensis Pristina /ongisoma Pristina lonf!iseta P. osborni Pristina so. Pristine/la sp. 28 Previous Collected in New in Collections 2016 2016 x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x

x x x x x x x x x x x x x x x x x x x x x x x x x x I I I I I Table 5.2 (continued)

Systematic List of Macroinvertebrates Collected From 1973 Through 2016 in The Ohio River Near BVPS Phylum I Class I Family Sub-Family Annelida Oligochaeta Naididae Tubificida Tubificidae Lumbriculidae Hirudinae Glossiphoniidae Ernobdellidae Haolotaxidae Lumbricina Lumbricidae 2016 Annual Environmental Report FENOC (BVPS) Genus and Species Pristinellajenkinae Pristine/la idrensis Pristine/la sima Pristina osborni Rivistes varasita Slavina avvendiculata Svecaria iosinae Stephensoniana trivandrana Sty/aria fossularis S. lacustris Uncinais uncinata Vejdovskyella comata Veidovskvella intermedia Veidovskye/la sp. Aulodrilus limnobius A. vizueti A. vluriseta Aulodrilus sp. Bothrioneurum veidovskyanum Branchiura sowerbvi Jlyodrilus templetoni Limnodrilus cervix L. cervix (variant)

L. claparedianus L. hoffineisteri L. maumeensis L. profi.mdicla L. sviralis L. udekemianus Limnodrilus sv. Peloscolex multisetosus lonzidentus P. m. multisetosus Potamothrix moldaviensis Potamothrix so. P. vejdovskyi Psammorvctides curvisetosus Tubifex tubifex Unidentified immature forms: with hair chaetae without hair chaetae Helobdella elongata H. staznalis Helobdella sp. Erpobdella sp. Mooreobdella microstoma Stylodrilus sp. 29 Previous Collected in New in Collections 2016 2016 x x x x x x x x x x x x x x x

x x x x x x x x x x x x x x x x x x

x x x x x x x x x x x x x x x

x x x x x x x x x x x x x x x x x Table 5.2 (continued)

T.i<:t nf M,.rrninvprf<>hr.ttP<:

C'nll<>,.fo.-1 Frnm 1973 Tl., .. v:. '?016 "n ThP Ohi< RiverN<> 1r BVPS Phylum Class Family Sub-Famil Arthronooa Acarina Ostracoda lsonooa Arthronoda Amphinoda Talitridae Gammaridae Pontoporeiidae Corophididae uecalJ()(Jll Lollemouia Eohemeroptera Heptageniidae Eohemeridae Baetidae Caenidae Tricorythidae Megaloptera Odonata Gomohidae Lestidae Libellulidae I t' 1 ecopte ra Trichoptera Hydropsychidae Hvdrootilidae Leptoceridae Polycentropodidae wo1ycentropoamae 2016 Annual Environmental Report FENOC (BVPS) Genus and Species Previous Collected in New in

?1116 ?111 /; x Oxus sp. x x Asel/us sp. x Hya/ella azteca x Crangonyx pseudograci/is x ICrangonyx sp. x Gammarus jasciatus x Gammarus sp. x x Monoporeia affinis x x x x x x x Stenacron sp. x Stenonema sp. x Ephemera sp. x Hexagenia so. x Ephron so. x Baetis sp. x Caenis sp. x x Serattelta sp. x Tricorythodes so. x Sia/is sp. x Argia so. x x Dromogomphus spoliatus x Dromogomphus sp. x Gomphus sp. x x Lestes so. x x Ubellula sp. x x x x x Cheumatopsyche sp. x Hydropsyche sp. x Parapsyche so. x Hydropti/a so. x Orthotrichia so. x Oxyethira sp. x x Cerac/ea so. x IUecetis sp. x x Cyrne//us so x iro1ycemropus sp. x 30 I u I ' I I I I Table 5.2 (continued) s . L" ;vstematic 1st o fM acromverte b rates C II d F 1973 Th 0 ecte rom rOU!! h 2016 . Th Oh" R" ID e 10 1ver N ear Phylum I Class Family Genus and Species Previous Collected in Sub-Famil r, .. 'Hitt.: Coleootera Hydrophilidae x Coleoptera Elmidae Ancyronyx variegatus x Dubiraphia sp. x x Helichus SD. x Optioserus sp. x Stene/mis SP. x Psephenidae x Diotera Unidentified Diptera x Psvchod1dae x Pericoma sp. x Psvchoda sp. x Telmatoscovus sp. x Unidentified Psychodidae ounae x Chaoboridae Chaoborus sp. x Simuliidae Similium sp. x Chironomidae x x Chrronornmae x Tanvtarsini oooa x Chironominae pupa x Axarus SP. x Chironomus sp. x x C/adope/ma sp. x C/adotanytarsus sp. x x Cryptochironomus sp. x x Cryptotendipes sp. x x Dicrotendipes nervosus x Dicrotendipes sp. x x Glvvtotendives SD. x Harnischia sp. x Microchironomus

• sp. x Micropsectra sp. x Microtendipes sp. x Parachironomus sp. x x Parac/adope/ma sp. x Paratanytarsus sp. x Paratendipes sp. x Phaenovsectra SP. x x Polypedi/um (s.s.) convictum type x Tanypodinae 2016 Annual Environmental Report FENOC (BVPS) P. (s.s.) simulans type Polvneditum sp. Pseudochironomis sp. Rheotanytarsus sp. Stempel/ina sp. Stenochironomus sp. Stictochironomus sv. Tanytarsus comnani Tanvtarsus sp. Tribelos sp. Xenochironomus sp. 1 anypodmae pupae Ablabesmvia sp. C/inotanypus sp. Coelotanvpus scapularis Coetotanypus sp. Dialmabatista pu/cher Dialmabatista sp. Procladius sp. 1anypus sp. 31 x x x x x x x x x x x x x x x x x x x x x x x x x x x x BVPS New in ')1\1 t.:

Table 5.2 (continued)

Systematic List of Macroinvertebrates Collected From 1973 Through 2016 in The Ohio River Near BVPS Phylum Class Family Sub-Family Diptera Tanvpodinae Orthocladiinae Diamesinae Ceratopogonidae Dolichopodidae Emoididae Ephydridae Muscidae Rhagionidae Tipu!idae Stratiomvidae Syrphidae Lepidoptera Hvdracarinidia Mollusca Gastroooda Hydrobiidae Anmicolinae Bithvnidae Physacea Pleuroceridae Phvsidae 2016 Annual Environmental Report FENOC (BVPS) Genus and Species Thienemannimvia group Zavrelimvia so. Ortlioc/adiinae pupae Cricotopus bicinctus C. (s.s.) trifi:Jscia Cricotopus (lsocladius)-sy/vestris Group C. (lsocladius) sp. Cricotovus (s.s.) sp. Eukiefferiella so. Hvdrobaenus SP. Limnovhves SP. Nanocladius (s.s.) distinctus Nanocladius sp. Orthocladius sp. Parametriocnemus sp. Paravhaenocladius sp. Psectrocladius sp. Pseudorthocladius sp. Pseudosmittia sp. Smittia sp. Theinemannimyia sp. Diamesa so. Potthastia sp. Probezzia so. Bezzia so. Culicoides so. Clinocera sp. Wiedemannia sp. Oxus sp. Amnicola sp. Aminicola binnevana Amnicola limosa Sta1<nicola elodes Bithvnia SP. Pleurocera acuta Goniobasis sp. Phvsa so. Phvsa ancillaria Phvsa infef!m 32 Previous.

Collected in New in Collections 2016 2016 x x x x x x x x x x x x x x x x x x x x x x x x x x x

x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x J ' ' ' J Table 5.2 (continued)

Systematic List of Macroinvertebrates Collected From 1973 Through 2016 in The Ohio River Near BVPS Phylum I Class Family Sub-Famil Mollusca Physacea Ancylidae Planorbidae Valvatidae Pelecyp0da IS phaeriacea Corbiculidae Sphaeriidae Dreissenidae Unionidae 2016 Annual Environmental Report FENOC (BVPS) Genus and Species Ferrissia sp. Gillia atilis Gvraulus sv. Valvata verdevressa Valvata viscinalis Valvata sincera Valvata sp. Corbiculafluminea Corbicula sp. Pisidium ventricosum Pisidium sp. Sphaerium sp. Unidentified immature Sphaeriidae Dreissena polymorpha Anodonta f!randis Anodonta (immature)

Ellivtio SP. IOuadrula vustulosa Unidentified immature Unionidae 33 Previous Collected in New in Collections 2016 2016 x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x Scientific name 1 Ablabesmyia sp. 0 Amnico/a sp. 0 Amnicota limosa 1 Argia (Odonata) 0 Aulodrilus sp 0 Branchiura sowerbyi 0 Caenis sp. 0 Ceratopogonidae 0 Chironomid pupae 1 Chironomidae 0 Chironomus sp. 1 Cladotanytarsus sp. 0 Coelotanypus sp. 0 Corbicu/a Sp. 1 Cricotopus (s.s.) sp. 0 Cryptochironomus sp. 1 Cryptotendipes sp. 0 Dicrotentipides sp 0 Dreissena polymorpha 0 Dubiraphia sp. 0 Enchytraeidae 0 Ephemeroptera 0 Femssia sp. 0 Gammarus sp. 0 Gyrau/us sp 0 Gomphus sp. 0 Goniobasis virginica 0 Hirudinea 0 Immature tubificid without 714 Lestes sp. 0 Limnodrifus cervix 0 Limnodrifus hoffmeisteri 18 Limnodrifus maumeensis 28 Limnodrifus udemekianus 0 Naididae 0 Nais communis 0 Na is pa rd a/is 0 Nais variabilis 0 Nematoda 1 Ocetis sp. 0 Oligochaeta 0 Orthocladius sp. 0 Oxyethira sp (Tricoptera) 0 Parachironomus sp. 0 Paranais sp 0 Phaenopsectra sp. 0 Physa sp 0 Pisidium sp. 0 P/eurocera acuta 0 Plecoptera 0 Polypedilum sp. 1 Pristina osbomi 2 Pristine/fa jenkinae 0 Pristine/fa sima 0 Proc/adius sp. 0 Pseudochironomis sp. 0 Stempellina sp. 0 Sty/aria /acustris 0 Tanytarsus sp. 0 Thienemannimyia group 0 Trichoptera 0 Tubificidae 0 Valvata sincera 0 Monthly Total 769 2016 Annual Environmental Report FENOC (BVPS) TA8LES.3 ----------------8El'ITHIC MACROINVERTEBRATECOUNTS FOR TRIPLICATESAMPLES TAKEN AT EACH SAMPLE STATION FORMAYANDSEPTEMBER2016 May Sept Location May Location 2A 281 282 283 3 Total 1 2A 281 282 0 0 0 0 0 0 1 0 6 3 0 0 2 0 1 3 0 0 20 2 0 0 0 0 0 1 3 1 5 1 0 0 0 0 0 0 0 0 8 0 0 2 0 1 5 8 0 0 0 0 0 4 0 1 0 5 0 1 1 2 0 0 0 0 0 0 4 3 12 1 0 0 0 0 0 0 0 0 0 0 3 4 0 0 3 11 6 4 6 2 0 0 0 0 0 0 0 0 0 1 0 34 1 3 9 48 5 0 0 0 0 0 0 0 0 0 0 45 3 0 0 0 0 0 1 1 0 0 0 2 0 4 7 1 1 14 7 1 10 8 0 0 0 0 0 0 0 0 17 0 0 9 1 2 2 15 1 11 5 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 2 4 84 35 15 5 9 1 91 4 99 204 2 12 456 137 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 1 6 3 3 0 1 8 3 1 0 13 0 0 4 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 1 0 0 1 0 1 2 0 0 200 31 204 49 1198 0 96 43 27 0 0 0 0 0 0 0 0 4 1 0 0 0 4 0 4 0 0 0 0 0 4 3 4 3 32 0 0 0 2 0 0 0 1 0 29 0 0 0 0 0 1 0 0 0 1 0 0 0 0 8 0 0 0 1 9 0 0 0 0 0 0 0 0 0 0 0 8 0 0 18 1 22 7 0 48 0 0 0 0 0 0 0 0 0 0 0 40 0 0 0 2 0 2 0 5 0 1 5 0 0 1 1 0 1 3 0 0 8 5 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 4 3 0 0 0 0 0 0 0 0 4 0 0 6 7 0 1 0 14 0 0 2 0 0 0 0 0 0 0 1 5 200 10 0 13 0 0 1 14 5 4 2 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 19 2 0 9 31 10 105 39 1 0 2 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 6 0 6 0 0 0 0 0 0 0 0 0 0 5 1 16 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 4 70 3 10 72 0 0 8 90 25 197 4 5 0 0 0 0 0 0 38 0 1 0 0 0 1 0 0 1 0 0 1 0 0 1 0 0 1 2 0 32 0 0 0 0 0 0 0 0 1 0 27 3 57 389 169 243 198 1825 205 619 1009 225 34 283 3 64 0 9 0 7 0 0 0 0 0 3 0 102 5 1 0 19 0 4 0 0 0 44 0 0 4 34 5 48 1 4 0 0 1 164 13 27 116 0 0 0 0 0 0 8 42 6 4 1 0 0 0 0 0 2 6 113 9 5 0 0 0 10 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12 0 0 0 4 0 47 2 0 1 1 0 0 0 101 1 8 0 0 2 1 0 56 1 2 0 0 0 0 0 136 0 0 0 0 0 15 17 148 1 0 3 0 0 0 0 4 7 1210 243 Sept Total 74 31 17 8 0 7 127 1 37 5 5 92 6 65 66 22 1 316 750 0 0 2 62 15 1 0 6 11 288 10 0 14 0 0 0 8 0 40 6 25 0 4 51 8 5 2 318 19 3 1 212 2 1 0 158 1 2 109 380 42 1 32 42 3511 2016 Total 74 34 18 8 8 12 127 1 48 5 53 92 7 79 66 37 1 320 954 1 1 2 63 28 1 1 6 12 1486 10 4 46 29 1 9 8 48 40 11 28 1 4 51 8 5 16 318 33 4 1 243 6 1 6 158 1 2 109 470 42 2 34 42 5336 I_ I u I I I May 1 (Control)

1. /m2 % Oligochaetes 10922 99 Chironomids 57 1 Mollusks 29 0 Others 14 0 Total 11022 100 September 1 (Control)

1. /m2 % Oligochaetes 0 0 Chironomids 2523 86 Mollusks 358 12 Others 57 2 Total 2938 100 2016 Annual Environmental Report FENOC (BVPS) TABLES.4 MFAN NUMBER OF MACRO INVERTEBRATES (NUMBER!M.2)

AND PERCENT COMPOSIDON OFOLIGOCHAEI'ES, CHIRONOMIDS, MOLLUSKS, AND OTHER ORGANISMS, 2016 BVPS Station 2A 2Bl (Non-control) 2B2 (Non-control) 2B3 (Non-control)

1. /m2 % #/rrf % #/m2 % #/m2 % #/m2 373 46 3082 55 817 34 3282 94 846 301 37 2078 37 57 2 86 2 487 129 16 258 5 1448 60 72 2 1476 14 2 158 3 100 4 43 1 29 -817 100 5576 100 2422 100 3483 100 2838 Station 2A 2Bl (Non-control) 2B2 (Non-control) 2B3 (Non-control)

1. /rrf % #/m2 % #/m2 % #/m2 % #/m2 2652 30 1648 11 487 15 2064 14 401 5776 65 1677 12 301 9 9904 66 358 373 4 10449 72 2322 72 2852 19 2480 78 1 688 5 115 4 100 1 244 8879 100 14462 100 3225 100 14920 100 3483 35 3 Total Mean % #/rrf % 30 3220 74 17 511 12 52 569 13 1 60 1 100 4360 100 3 Total Mean % #/rrf % 12 1209 14 IO 3423 41 71 3139 37 7 616 7 100 8387 100 TABLE5.5 MEAN NUMBER OF MACROINVERTEBRATES (NUMBER/M 2) AND PERCENT COMPOSITION OF OLIGOCHAETA, CHIRONOMIDAE, MOLLUSCA, AND OTHER ORGANISMS FOR THE CONTROL STATION (1) AND THE AVERAGE FOR NON-CONTROL STATIONS ( 281, 282, AND 283), 2016 BVPS May Control Station (Mean) #/m2 % Oligochaeta 10922 99 Chironomidae 57 1 Mollusca 29 0 Others 14 0 TOTAL 11022 100 September Control Station (Mean) #/m2 Oligochaeta 0 Chironomidae 2523 Mollusca 358 Others 57 TOTAL 2938 2016 Annual Environmental Report FENOC (BVPS) % 0 86 12 2 100 Non-Control Station (Mean) #/m2 % 2394 63 740 19 593 15 100 3 3827 100 Non-Control Station (Mean) #/m2 % 1400 13 3961 36 5208 48 301 3 10869 100 36 J I I , J i ' I ' I I I I I I I ' I I I_ TABLE5.6 SHANNON-WEINER DIVERSITY, EVENNESS AND RICHNESS FOR BEN111IC MACROINVERTEBRATES COLLECTED IN IBEOHIO RNER, 2016 .. May 1 '*. c *',' ,, No.ofTaxa* " 11 Weiner Index 0.15 Eveiiµess 0.15 ... ,* Richness 0.75 .. ... ' : : Septeinber

.1 No. ofTaxa 18 fude;x:

• 0.86 Evenness 0.69 Richness " 3.76 2016 Annual Environmental Report FENOC (BVPS) 21\. 8 0.79 0.87 1.73 *'. 2A 24 0.94 0.68 2.80 Station'.*

• -" . 2Bl 2B2 2B3 ' .3. 20 16 16 19 0.74 0.67 0.36 0.72 0.57 0.55 0.30 0.56 2.52 2.73 1.27 3.40 .::Station

' ... *.2BI. '* 2B2 *2B3 3 35 24 34 21 0.89 0.72 1.21 0.83 0.58 0.52 0.79 0.63 4.48 2.77 4.65 4.19 37 ------------

Table 5.7. Benthic Macroinvertebrate Densities for Stations 1 (Control) and 28 (Noncontrol), BVPS, 1973-2016.

1973 1 May 248 August 99 Mean 173 1976 1 May 927 August 851 Mean 889 1979 1 May 1004 Aug/Sept 1185 Mean 1095 1982 1 May 3490 September 2958 Mean 3223 1985 1 May 2256 September 1024 Mean 1640 2016 Annual Environmental Report FENOC (BVPS) 28 508 244 376 28 3660 785 2223 28 840 588 714 28 3026 3364 3195 28 867 913 890 Preoperational 1974 1975 .1 28 1 28 1116 2197 143 541 1017 1124 630 1369 1017 1124 Operational 1977 1978 1 28 1 28 674 848 351 126 591 3474 601 1896 633 2161 476 1011 Operational 1980 1981 1 28' 1 28 1041 747 209 456 1523 448 2185 912 1282 598 1197 684 Operational 1983 1984 1 28 1 28 3590 1314 2741 621 4172 4213 1341 828 3881 2764 2041 725 Operational 1986 1987 1 28 1 28 601 969 1971 2649 849 943 2910 2780 725 956 2440 2714 38 I I I i ' ' Table 5.7. Benthic Macroinvertebrate Densities for Stations 1 (Control) and 28 (Noncontrol), BVPS, 1973-2016 (Continued).

1988 ** 1* 28;* May 1804 1775 September 1420 1514 Mean 1612 1645 " 1991 ' 1 2a* May 7760 6355 September 3588 2605 Mean 5808 4480 . '*. ** 1994 1 '28 May 6980 2349 September 1371 2930 Mean 4176 2640 .. 1997 1 28*. May 1411 2520 September 1944 2774 Mean 1678 2647 2000 .1 May 2987 September 3092 Mean 3040 2016 Annual Environmental Report FENOC (BVPS) 28 2881 2742 2812 . ' Operational

. '

1990 .. ' 1 ., 28 .: 1 ' 28 ". 3459 2335 15135 5796 1560 4707 5550 1118 2510 3274 10343 3457 Operational

.. 1992 1993 1 *28: ' . 1 ' 28*' 7314 10560 8435 2152 2723 4707 4693 2143 5019 7634 6564 2148

• Operational . , . 1995 1996 1 28 1 '28* 8083 9283 1987 1333 1669 3873 1649 2413 4876 6578 1814 1873 Operational . ': .. . . 1998 1999 1 *, 28 . '.. 1 .29; 6980 2349 879 1002 1371 2930 302 402 4176 2640 591 702 Operational

• "" 2001 2002 1 ;, 28 1 .28 3139 5232 1548 2795 8632 14663 3139 5232 5090 8729 39

.l Table 5.7. Benthic Macroinvertebrate Densities for Stations 1 (Control) and 28 (Noncontrol), BVPS, 1973-2016 (Continued).

2003' 1.' May 7095 September 2193 Mean 4644 2006 1 May 143 September 229 Mean 186 2009 f May 71 September 903 Mean 487 2Q12 .*, .1 May 71 September 903 Mean 487 2016 Annual Environmental Report FENOC (BVPS) 28. 10750 6464 8607 28 1242 2199 1721 28 1462 1902 1682 28 1462 1902 1682 , '*.' Operational

'2004 2005 1 28 :*1 ,. *29. 2752 4558 516 1146 10062 7604 4773 6435 6407 6181 2645 3791 . Operational

• .. 2007 2008 1 28 1 28 559 912 158 1252 560 3794 1161 2150 560 2353 660 1701 Operath:mal . 2010 -. 2011 1 28 1 28 1763 2527 115 1700 1720 1256 874 1233 1742 1892 495 1467 Operational*

2013 2014 1 .. 28. 1 ' . 28 2107 903 1634 3149 373 1731 3526 7310 1240 1317 2580 5230 40 I , ] . l, J ' '

TABLES.8 TOTAL FISH CATCH; ELECTROFISHING AND SEINE NET COMBINED DURING THE BVPS 2016 FISHERIES SURVEY Common Name .. Scientific Name .. Smalhnouth buffalo Jctiobus bubalus Black crappie Pomoxis nigromaculatus Bluegill Lepomis macrochirus Carp Cyprinis carpio Channel catfish Ictalurus punctatus Freshwater drum Aplodinotus grunniens Gizzard shad Dorosoma cepedianum Golden redhorse sucker Moxostoma erythrurum Largemouth bass Micropterus salmoides Longnose gar Lepisosteus osseus Pumpkinseed Lepomis gibbosus Quillback Carpiodes cyprinus Sauger Sander canadense Shorthead redhorse sucker Moxostoma macrolepidotum Silver redhorse sucker Moxostoma anisurum Smalhnouth bass Micropterus dolomieu Spotted bass Micropterus punctulatus Walleye Sander vitreum Yellow perch Perea flavescens

!Total Fish Collected in 2016 2016 Annual Environmental Report FENOC (BVPS) 42 Number. II Percent . I 28 20.29 2 1.45 3 2.17 19 13.77 1 0.72 4 2.90 15 10.87 21 15.22 4 2.90 4 2.90 7 5.07 1 0.72 2 1.45 9 6.52 3 2.17 5 3.62 5 3.62 1 0.72 4 2.90 138 100.00 TABLES.9 COMPARISON OF CONTROL VS. NON-CONTROL ELECTROFISIDNG CATCHES DURING THE BVPS 2016 FISHERIES SURVEY Common Name Control Smallmouth buffalo Black crappie Bluegill Carp Channel catfish Freshwater drum Gizzard shad Golden redhorse sucker Largemouth bass Longnose gar Pumpkinseed Quillback Sauger Shorthead redhorse sucker Silver redhorse sucker Smallmouth bass Spotted bass Walleye Yellow perch I Total II 2016 Annual Environmental Report FENOC (BVPS) 13 5 1 1 2 5 1 2 4 1 3 38 O/o 34.21 13.16 2.63 2.63 5.26 13.16 2.63 5.26 10.53 2.63 7.89 I 100.00 I Non-control

% Total fish 0 io 15 15.0 28 20.29 2 2.0 2 1.45 3 3.0 3 2.17 14 14.0 19 13.77 1 0.72 3 3.0 4 2.90 13 13.0 15 10.87 16 16.0 21 15.22 3 3.0 4 2.90 4 4.0 4 2.90 5 5.0 7 5.07 1 1.0 1 0.72 2 2.0 2 1.45 5 5.0 9 6.52 3 3.0 3 2.17 5 5.0 5 3.62 4 4.0 5 3.62 1 1.0 1 0.72 1 1.0 4 2.90 100 100.0 138 100.00 43 I I I l I I I I I TABLES.IO FISH SPECIES COLLECTED DURING THE MAY2016 (SPRING) SAMPLING OF THE OHIO RIVER IN THE VICINITY OF BVPS Sample locations

• Seine

• Electrofishim?

Common Name S-1** S-2** E-1 E-2A E-2B Smalhnouth buffulo Black crappie Bluegill 2 Carp I Channel catfish Freshwater dnnn I Gizzard shad Golden redhorse sucker 2 Largemouth bass Longnose gar Pumpkinseed I Quillback Sauger Shorthead redhorse sucker 4 Silver redhorse sucker Smalhnouth bass I Spotted bass Walleye I Yellow perch Total I 0 0 4 3 6 *Gear= (E) Fish captured by electrofishing; (S) captured by seining **Not sampled due to safety concerns 2016 Annual Environmental Report FENOC (BVPS) 44 E-3 I 10 I 12 Total . O/o Total % 0 -I 4.00 0 -0 0.00 0 -2 8.00 0 -I 4.00 0 -0 0.00 0 -I 4.00 0 -IO 40.00 0 -2 8.00 0 -0 0.00 0 -0 0.00 0 -I 4.00 0 -0 0.00 0 -0 0.00 0 -5 20.00 0 -0 0.00 0 -I 4.00 0 -0 0.00 0 -I 4.00 0 -0 0.00 0 -25 100.00 TABLE5.11 F1SH SPECIES COLLECTED DURING THE JULY (SUMMER) 2016 SAMPLING OF THE omo RIVER IN THE VICINITY OF BVPS Sample locations

• Seine Electrofishing Common Name S-1** S-2** E-1 E-2A E-2B Smallmouth buffalo 3 I Black crappie ' Bluegill Carp Channel catfish Freshwater drum Gizzard shad Golden redhorse sucker Largemouth bass Longnose gar 2 Pumpkinseed Quillback Sauger 1 Shorthead redhorse sucker Silver redhorse sucker Smallmouth bass I Spotted bass Walleye Yellow oerch Total 0 0 4 4 *Gear= (E) Fish captured by electrofishing; (S) captured by seining **Not sampled due to safety concerns 2016 Annual Environmental Report FENOC (BVPS) 45 3 I 4 E-3 Total % Total % I 0 -8 61.54 0 -0 0.00 0 -0 0.00 0 -I 7.69 0 -0 0.00 0 -0 0.00 0 -0 0.00 0 -0 0.00 0 -0 0.00 0 -2 15.38 0 -0 0.00 0 -0 0.00 0 -1 7.69 0 -0 0.00 0 -0 0.00 0 -I 7.69 0 -0 0.00 0 -0 0.00 0 -0 0.00 1 0 -13 100.00 I I I TABLE5.12 FISH SPECIES COLLECTED DURING THE SEPTEMBER (FALL) 2016 SAMPLING OF THE omo RIVER IN THE VICINITY OF BVPS Sample locations

• Common Name S-1 ** S-2** E-1 E-2A E:..2B Smallmouth buffalo 3 Black crappie Bluegill 1 Carp Channel catfish I Freshwater drum Gizzard shad 4 I Golden redhorse sucker I I Largemouth bass Longnose gar 2 1 Pumpkinseed Quillback Sauger Shorthead redhorse sucker Silver redhorse sucker Smallmouth bass 1 Spotted bass Walleye Yellow nerch !Total lolol11l s

• Gear= (E) Fish captured by electrofishing; (S) captured by seining **Not sampled due to safety concerns 2016 Annual Environmental Report FENOC (BVPS) 46 1 6 1 8 E-3 10 1 1 12 Seine

• Electrofishing Total % Total O/o. 0 -4 11.11 0 -0 0.00 0 -1 2.78 0 -0 0.00 0 -I 2.78. 0 -0 0.00 0 -21 58.33 0 -2 5.56 0 -0 0.00 0 -4 11.11 0 -0 0.00 0 -1 2.78 0 -0 0.00 0 -0 0.00 0 -0 0.00 0 -2 5.56 0 -0 0.00 0 -0 0.00 0 -0 0.00 0 36 J 100.00 I TABLES.13 FISH SPECIES COLLECTED DURING THE NOVEMBER (WINTER) 2016 SAMPLING OF THE omo RIVER IN THE VICINITY OF BVPS Sample locations

• Common Nanie S-1** S-2** E-1 E-2A Smalhnouth buffalo 7 2 Black crappie Bluegill Carp 5 4 Channel catfJSh Freshwater drum 1 1 Gizzard shad 2 Golden redhorse sucker 1 2 Largemouth bass Longnose gar 2 Pumpkinseed Quillback Sauger 1 Shorthead redhorse sucker 1 Silver redhorse sucker 2 Smallmouth bass 1 Spotted bass Walleye Yellow perch 3 1 !Total I o I o I 19 I 11

• Gear= (E) Fish captured by electrofishing; (S) captured by seining **Not sampled due to safety concerns 2016 Annual Environmental Report FENOC (BVPS) 47 2 2 1 2 1 1 1 2 1 2 1 16 .. *seine

'. . ' E-3 Total '% Total % 2 0 -13 20.31 1 0 -1 1.56 0 -0 0.00 3 0 -14 21.88 0 -0 0.00 0 -3 4.69 3 0 -5 7.81 0 -5 7.81 2 0 -2 3.13 0 -2 3.13 0 -1 1.56 0 -1 1.56 0 -2 3.13 0 -3 4.69 0 -3 4.69 I 0 -4 6.25 0 -1 1.56 0 -0 0.00 0 -4 6.25 12 0 0 64 100 ] I I I _ _J l i ' r I TABLES.14

• ESTIMATED NUMBER OF FISH OBSERVED DURING ELECTROFISIDNG OPERATIONS, 2016 Common Name Bluegill Emerald Shiner Freshwater dnnn Smallmouth bu:ffulo Longnose gar Smallmouth bass Unidentified suckers Unidentified sunfish Walleye/Sauger Yellow perch Gizzard shad Total * =Not boated or handled 2016 Annual Environmental Report FENOC (BVPS) I May 1 2 2 1 1 10 17 I July I Sept I Nov 1000+ 100+ 1 1 3 1 1 3 1 1 2 1000+ 10 100+ 48 I Total I 0 1100+ 1 3 4 1 3 4 2 Table 5.15 CATCH PER UNIT EFFORT (CPUE AS FISH/ELECTROFISHING MINUTE) BY SEASON DURING THE BVPS 2013 FISHERIES SURVEY Season Effort (min) Common Name Spring 40.4 Smalhnouth buffitlo Black crappie Bluegill Gizzard shad Golden redhorse sucker Longnose gar Prnnpkinseed Quillback River carpsucker Rock bass Shorthead redhorse sucker Season Effort (min) Summer 40.0 2016 Annual Environmental Report FENOC (BVPS) Smalhnouth bass Spotted bass Season Total .. Common Na111e

• Smalhnouth buffitlo Black crappie Gizzard shad Golden redhorse sucker Sauger Smalhnouth bass Season Total 49 Number *CPUE (fish/min)

Collected 1 0.0248 1 0.0248 1 0.0248 1 0.0248 8 0.1980 2 0.0495 1 0.0248 2 0.0495 2 0.0495 1 0.0248 10 0.2475 7 0.1733 2 0.0495 39 0.9653 Number ColleCted.

CPUE (fish/min) 3 0.0750 1 0.0250 1 0.0250 3 0.0750 1 0.0250 2 0.0500 11 0.2750 _) J ] I I .l r 0 Table 5.15 (continued)

CATCH PER UNIT EFFORT (CPUE AS FISH/ELECTROFISHING MINUTE) BY SEASON DURING THE BVPS 2013 FISHERIES SURVEY Season Effort (min) Common Name Fall 40.4 Bluegill Channel catfish Flathead catfish Freshwater dnnn Gizzard shad Golden redhorse sucker Longnose gar River carpsucker Smalhnouth bass Spotted bass Walleye *Season Total ,. Season Effort (min) *common.Name Winter 40.1 Bluegill Channel catfish Freshwater dnnn Golden redhorse sucker Rock bass Shorthead redhorse sucker 2013 160.9 2016 Annual Environmental Report FENOC (BVPS) Smalhnouth bass Yellow perch Season Total 50 Number * .. CPUE (fish/min)

• -Collected 2 0.0495 1 0.0248 1 0.0248 1 0.0248 I 0.0248 2 0.0495 1 0.0248 1 0.0248 1 0.0248 1 0.0248 1 . 0.0248 13 0.3218 Number *CPUE {fISh!min) 1 0.0249 1 0.0249 1 0.0249 6 0.1496 3 0.0748 7 0.1746 2 0.0499 1 0.0249 22 0.5486 85 .*. 0.52828 Table 5.16 CATCH PER UNIT EFFORT (CPUE AS FISH/ELECTROFISHING MINUTE) BY SEASON DURING THE BVPS 2014 FISHERIES SURVEY Season Effort (min) Spring 40.4 *Season *

• Effort (min) SlU1llller 40.1 2016 Annual Environmental Report FENOC (BVPS) Common Name Smalhnouth buffalo Carp Channel catfish Freshwater dnnn Gizzard shad Golden redhorse sucker Longnose gar River carpsucker Shorthead redhorse sucker Smalhnouth bass Spotted bass Walleye Season Total .. Common Name -.* --Smalhnouth buffalo Carp Gizzard shad Longnose gar Shorthead redhorse sucker Smalhnouth bass Season Total 51 Number CPUE (fish/min)

Collected*

3 0.0743 2 0.0495 2 0.0495 2 0.0495 12 0.2970 3 0.0743 4 0.0990 1 0.0248 4 0.0990 4 0.0990 2 0.0495 5 0.1238 44 1.0891 Number Collected . CPUE (fish/min) 6 0.1496 1 0.0249 14 0.3491 1 0.0249 3 0.0748 1 0.0249 26 0.6484 l L Table 5.16 (continued)

CATCH PER UNIT EFFORT (CPUE AS FISH/ELECTROFISHING MINUTE) BY SEASON DURING THE BVPS 2014 FISHERIES SURVEY Season Effort (min) Fall 40.0 Season Effort (min) Winter 40.0 I 2014 I 160.5 I 2016 Annual Environmental Report FENOC (BVPS) Common Name Carp Gizzard shad Largemouth bass Shorthead redhorse sucker Yellow perch Season Total Common Name Smalhnouth buffiilo Bluegill Carp Gizzard shad Golden redhorse sucker Longnose gar Shorthead redhorse sucker Smalhnouth bass Season Total 52 Number CPUE (tis bf min) CoUected 4 0.1000 26 0.6500 1 0.0250 2 0.0500 1 0.0250 34 0.8500 Number CPUE (fi"' h/min) Collected 4 0.1000 1 0.0250 6 0.1500 13 0.3250 4 0.1000 4 0.1000 1 0.0250 1 0.0250 34 0.8500 I 138 I 0.85981 I Table 5.17 CATCH PER UNIT EFFORT (CPUE AS FISH/ELECTROFISHING MINUTE) BY SEASON DURING THE BVPS 2015 FISHERIES SURVEY Season Effort (min) Common Name Spring 41.5 Smalhnouth buffulo Carp Flathead catfish Freshwater dnnn Goldfish Golden redhorse sucker Longnose gar Quillback Rock bass Shorthead redhorse sucker

• Season Effort (min) Summer 40.7 2016 Annual Environmental Report FENOC (BVPS) Smalhnouth bass Spotted bass Walleye Season Total Common Name Smalhnouth buffulo Flathead catfish Gizzard shad Longnose gar Pmnpkinseed Smalhnouth bass Season Total 53 Number CPUE Collected (fish/min) 2 0.0482 1 0.0241 1 0.0241 3 0.0723 1 0.0241 7 0.1687 6 0.1446 1 0.0241 1 0.0241 2 0.0482 3 0.0723 1 0.0241 1 0.0241 30 0.7229 Number CPUE Collected (ftshlniin) 1 0.0246 1 0.0246 9 0.2211 2 0.0491 1 0.0246 1 0.0246 15 0.3686 r

' ' ' ' Table 5.17 (continued)

CATCH PER UNIT EFFORT (CPUE AS FISH/ELECTROFISHING MINUTE) BY SEASON DURING THE BVPS 2015 FISHERIES SURVEY Season Eft'ort (D1in)' Common Name Number II ColleCted Fall 40.9 Bluegill Gizzard shad Longnose gar Pmnpkinseed Season Total Season Effort' (min) Common Name Winter 40.6 Smallmouth buffulo Channel catfish Freshwater dnnn Gizzard shad Longnose gar Shorthead redhorse sucker I 2015 I 163.7 I 2016 Annual Environmental Report FENOC (BVPS) Smalhnouth bass Season Total 54 1 0.0244 286 6.9927 2 0.0489 2 0.0489 291 7.1149 Number CPUE " .* Coilected (fis hf min). 17 0.4187 1 0.0246 1 0.0246 60 1.4778 1 0.0246 1 0.0246 2 0.0493 83 2.0443 I 419 I 2.55956 I Table 5.18 CATCH PER UNIT EFFORT (CPUE AS FISH/ELECTROFISIDNG MINUTE) BY SEASON DURING THE BVPS 2016 FISHERIES SURVEY Season Effort (min) . Common Name Spring 41.2 Smallmouth buffalo Bluegill Carp Freshwater drum Gizzard shad Golden redhorse sucker Pumpkinseed Shorthead redhorse sucker Season Effort (min) Summer 40.8 2016 Annual Environmental Report FENOC (BVPS) Smallmouth bass Walleye Season Total Common Name Smallmouth buffalo Carp Longnose gar Sauger Smallmouth bass Season Total 55 Number CPUE (fish/inin)

Collected 1 0.0243 2 0.0485 1 0.0243 1 0.0243 10 0.2427 2 0.0485 1 0.0243 5 0.1214 1 0.0243 1 0.0243 25 0.6068 Number CPUE (fish/min)

Collected 8 0.1961 1 0.0245 2 0.0490 1 0.0245 1 0.0245 13 0.3186 I J I -Table 5.18 (continued)

CATCH PER UNIT EFFORT (CPUE AS FISH/ELECTROFISIDNG MINUTE) BY SEASON DURING THE BVPS 2016 FISHERIES SURVEY Season-Effurt (min) Common Name Fall 42.2 Smallmouth buffalo Bluegill Channel catfish Gizzard shad Golden redhorse sucker Longnose gar Quillback Smallmouth bass Season Total Season Effort (min) Common Name Winter 41.1 Smallmouth buffalo Black crappie Carp Freshwater drum Gizzard shad Golden redhorse sucker Largemouth bass Longnose gar Pumpkinseed Quillback Sauger Shorthead redhorse sucker Silver redhorse sucker Smallmouth bass Spotted bass Yellow perch Season Total 2016 165.3 2016 Annual Environmental Report FENOC (BVPS) . 56

• Number CPUE (:fish/min)

Collected 4 0.0948 I 0.0237 1 0.0237 21 0.4976 2 0.0474 4 0.0948 1 0.0237 2 0.0474 36 0.8531 Number CPUE (fish/min)

Collected 13 0.3163 I 0.0243 14 0.3406 3 0.0730 5 0.1217 5 0.1217 2 0.0487 2 0.0487 1 0.0243 1 0.0243 2 0.0487 3 0.0730 3 0.0730 4 0.0973 1 0.0243 4 0.0973 64 1.5572 138 0.83485 TABLES.19 UNIT 1 COOLING RESERVOIR MONTHLY SAMPLING CORBICULA DENSITY DATA FOR 2016 FROM BVPS Area Collection Sampled Live or Date (sq ft) Dead 3/30/2016 0.25 Dead Live 4/28/2016 Dead 0.25 Live 5/31/2016 0.25* Dead Live 6/30/2016 0.25 Dead Live 7/27/2016 0.25 Dead Live 8/25/2016 0.25 Dead Live 9/13/2016 0.25 Dead Live 10/27/2016 0.25 Dead Live Dead 111212016 0.25 Live Unit summary Dead Live 2016 Annual Environmental Report FENOC (BVPS) Maximum Length Count Range (mm) 0 ---1 2.00-3.34 0 ---0 ---1 >9.50 0 ---0 ---0 ---3 4.75-6.29 0 ---0 ---0 ---2 4.75-6.29 0 ---0 ---0 ---0 ---0 ---6 >9.50 1 2.00-3.34 57 Minimum Estimated Length Number Range(mm) (per sq m) ---0 2.00-3.34 43 ---0 ---0 >9.50 43 ---0 ---0 ---0 2.00-3.34 129 ---0 ---0 ---0 2.00-3.34 86 ---0 ---0 ---0 ---0 ---0 2.00-3.34 29 2.00-3.34 5

I -TABLES.20 UNIT 2 COOLING RESERVOIR MONTHLY SAMPLING CORBICULA DENSITY DATA FOR 2016 FROM BVPS Area* Collection Sampled Live or

• Date (sq ft) Dead 3/30/2016 0.25 Dead* Live 4/28/2016 0.25 Dead Live 5/31/2016 0.25 Dead Live 6/30/2016 0.25 Dead Live 7/27/2016 0.25 Dead Live 8/25/2016 0.25 Dead Live 9/13/2016 0.25 Dead Live 10/27/2016 0.25 Dead Live 11/2/2016 Dead 0.25 Live Unit summar Dead Live 2016 Annual Environmental Report FENOC (BVPS)

• Maximum Le'ngth Range Count (min) 0 ---0 ---0 ---2 2.00-3.34 1 >9.50 2 6.30-9.94 0 ---0 ---1 4.75-6.29 0 ---0 ---0 ---2 4.75-6.29 0 ---0 ---0 ---3 6.30-9.94 4 6.30-9.94 7 >9.50 8 6.30-9.94 58 Mip.imuni F.stimated

Length Number*. Range( mm) . (persqm) ---0 ---0 ---0 2.00-3.34 86 >9.50 43 3.35-4.74 86 ---0 ---0 4.75-6.29 43 ---0 ---0 ---0 2.00-3.34 86 ---0 ---0 ---0 4.75-6.29 129 4.75-6.29 172 2.00-3.34 33 2.00-3.34 38 2016 Annual Environmental Report FENOC (BVPS) 9.0 FIGURES 59 1000 Mtler*----j Figure 5.1 2016 Beaver Valley Power Station Aquatic Monitoring Program Sampling Control and Non-Control Sampling Stations 2016 Annual Environmental Report FENOC (BVPS) 60 LEGEND jBenthlc sample siie i I j --------------Mag14.00 n.. .Mn 16 10:231997 Selle 1 :31,250 (II center) 2000Feet t----*-t 1000-* _. *---*---l Figure 5.2 Location Map for Beaver Valley Power Station Benthic Organism Survey Sampling Sites for the 2016 Study 2016 Annual Environmental Report 61 F NO (BVP )

• --* ... *--*-***-----=-----""" LEGEND

• Electrolishing sile " Seine silt: M"IJ 14.00 Thu Jan 18 10: 14 1ll97 -1: 31 , 250(0l....iot) 20DOFHI t---*-** **--1 1000Mete<*

t------Figure 5.3 Location Map for Beaver Valley Power Station Fish Population Survey Fish Sampling Sites for the 2016 Study 2016 Annual Environmental Report FENOC (BVPS) 62 Figure 5.4 2016 Annual Environmental Report FENOC (BVPS) Mon U.17 12:4519117 scm. 1 :Q,500 (Ill.....,, 1 ..... 2KM 0 19'5 DcLan. Location of Study Area , Beaver Valley Power Station Shippingport , Pennsylvania BVPS 63 z c rn z s ::a 0 z rn ,... ::a m "'O 0 ::a -t Comparison of live Corbicula clam density estimates among 2016 BVPS Unit 1 cooling tower reservoir events, for various clam shell groups. 500 ii:: 450 w Iii 400 :IE a: w 350 a.. I 300 250 ... 200 0 a: 150 w "" 2' 100 :::> z 50 0 3130 *0.01-0.99 mm 0 o 1.00-1.99 mm 0 *2.00-3.34 mm 43 *3.35-4. 74 mm 0 *4. 75-6.29 mm 0 *6.30-9.49 mm 0 o>9.50mm 0 TOTAL #lm2 43 2016 Annual Environmental Report FENOC (BVPS) 4128 0 0 0 0 0 0 0 0 5131 6130 7127 8125 9113 10127 1112 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Figure 5.5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 64 SIZE RANGE Comparison of live Corbicula clam density estimates among 2016 BVPS Unit 2 cooling tower reservoir events, for various clam shell groups. ii:: w I-w :::!: 500 0:: w 0.. 450 < ...J 400 :J 0 350 iii 0:: 0 300 0 u. 250 0 0:: 200 w Ill 150 :::!: :J z 100 50 0 *0.01-0.99 mm 01.00-1.99 mm 0 *2.00-3.34 mm 0 *3.35-4. 7 4 mm 0 *4.75-6. 29 mm 0 *6.30-9. 49 mm 0 o>9.50mm 0 TOTAL #An2 0 2016 Annual Environmental Report FENOC (BVPS) 0 0 86 0 0 43 0 0 0 43 0 0 86 86 SIZE RANGE 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 43 0 0 0 0 0 129 0 0 0 0 0 0 0 0 0 0 0 172 Figure 5.6 65 -----

Comparison of live Corbicula clam density estimates among 2016 BVPS Intake Structure sample events, for various clam shell groups. 2016 Annual Environmental Report FENOC (BVPS) 7 1 27 0 0 3 1 0 6 66 9 1 13 0 0 0 0 1 0 2 3 SIZE RANGE 11/ 2 0 0 0 0 Intake structure bottom samples are 0 collected from the Oh i o River at the Intake Bu i lding. 1 2 3 Figure 5.7 Water Temperature and River Elevation Recorded at the Ohio River at BVPS Intake Structure During 2016 on Monthly Sample Dates. 90 80 70 ::i "E G> a. 60 E Q) ':: Q) 10 3:: 50 40 30 3130 Figure 5.8 2 016 Annual Environm e nt a l Report FEN O C (BVP ) 4128 5/31 676 674 672 670 668 666 -temp --+--elevation 664 6/30 7127 8125 9/13 10/27 11/2 2016 Monthly Sample Dates 67 ::0 < m CD" < 0 ::i -

14000 12000 10000 8000 6000 4 000 2000 0 I n tak e Structur e/Open W at er Un i t 1 C oolin g T o w er R eseiv oir Unit 2 Coolin g Tower R eseivoi r 11 1412 8 0 0 0 05/3 1 3 0 0 0 0 6'3 0 0 1 0 0 *7 1 27 1 00 80 34 10 1 2 460 812 5 4 9 2 0 1590 2 91 0 *9 1 13 11 0 320 1 20 a 10 121 0 0 0 Sample location Figure 5.9. Density of zebra mussel veligers collected at Beaver Valley Power Station, 2016. 2016 Annual Environmental Report FENOC (BVPS) 68 160000 140000 120000 100000 .... s 80000 :if; 60000 40000 20000 0 Barge Slip 124/2 8 0 05/31 0 06/30 180 *7127 640 08/25 1310 *9 1 1 3 73 0 010/27 0 Splash Pool 0 50 30 136250 20500 640 10 Sample location Emergency Outfall Facility 0 190 260 87167 15060 590 0 Figure 5.10. Density of zebra mussel veligers collected at Beaver Valley Power Station, 2016. 2016 Annual Environmental Report FENOC (BVPS) 69 I' I 17.0 16.0 15.0 14.0 13.0 12.0 11.0 10.0 N 9.0 5 8.0 :tt; 7.0 6.0 5.0 4.0 3.0 2.0 1.0 0.0 83/3 0 r<l 4128 05/31 06/3 0 07/2 7 08/25 *9 1 13

• 10127

• 1112 Intake Structure/Open Water 3.6 2.2 15.2 0.4 6.3 0.9 12.1 1.8 3.6 Unit 1 Cooling Tower Reservoir 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Unit 2 Cooling Tower Reservoir 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Figure 5.11. Density of settled zebra mussels at Beaver Valley Power Station, 2016. 2016 Annual Environmental Report FENOC (BVPS) 70 40.00 35.00 30.00 25.00 M 20.00 15.00 10.00 5.00 0.00 Barge Slip Spla s h Pool Emergenc y Outfall Facility r.?J3/30 3 5.87 0.4 5 0.00 04/28 2 4.2 2 0.00 0.00 05/3 1 7.6 2 0.00 0.00 *6 1 30 5.8 3 0.00 0.00 *7 12 7 11.2 1 0.00 0.00 *8 1 25 4.9 3 0.00 0.00 *9 1 13 1 3.90 0.00 0.00 10/27 7.17 0.00 0.00 011 12 18.3 9 0.00 0.00 Figure 5.12. Density of settled zebra mussels at Beaver Valley Power Station, 2016. 2 016 Annua l Environmental Report FENOC (BVPS) 71 I I I I 2016 Annual Environmental Report FENOC (BVPS) 10.0 PERMITS 72 Attachment 10.1: PERMITS & CERTIFICATES FOR ENVIRONMENTAL COMPLIANCE Registration Number Re1mlator/Descrintion Exniration BVPS EPA generator identification Resource Conservation

& Recovery Act (RCRA) Identification number for regulated waste activity.

Also used by PA DEP Indefinite P AR000040485 to monitor regulated waste activity under the Pennsylvania Solid Waste Management Act (SWMA). 04-02474 BVPS EPA Facility Identification Number for CERCLA/EPCRA/SARA.

Used for Indefinite SARA Tier II reporting and emergency planning.

FE Long Term Distribution Center/Warehouse (22) EPA Facility Identification 04-02475 Number for CERCLA/EPCRA/SARA.

Used for SARA Tier II reporting and Indefinite emergency planning.

12/27/2006 Continued PA0025615 BVPS NPDES Permit number under US EPA and PA DEP. pending approval of renewal application.

04-13281 BVPS Unit 1 PA DEP Facility Identification

& certificate number for regulated Indefinite storage tanks. 04-13361 BVPS Unit 2 PA DEP Facility Identification

& certificate number for regulated Indefinite storage tanks. OP-04-00086 PA DEP State Only Synthetic Minor Permit for emergency auxiliary boilers, 04/28/2020 emergency diesel generators, paint shop and other miscellaneous sources. NIA PA DEP Open Burning Permit for operation of the BVPS Fire School-annual 01/01/2018 application and renewal 042009 450 002RT US Department of Transportation Hazardous Materials Registration 06/30/2018 200100242 US Army Permit for maintenance dredging (With Encroachment/Submerged Lands 12/31/2021 Agreement

1. 0477705, this allows maintenance dredging.).

Encroachment Permit/Submerged Lands Agreement for construction and 0477705 maintenance of current barge slip. (With US Army Permit #200100242, this allows Indefinite maintenance dredging.)

06786A Encroachment Permit/Submerged Lands Agreement for transmission line over Ohio Indefinite River (al, Mile 34.5 18737 Encroachment Permit/Submerged Lands Agreement for Unit 1 intake and discharge Indefinite (main combined intake and outfall structures) 0475711 Encroachment Permit/Submerged Lands Agreement for construction and Indefinite maintenance of Unit 2 auxiliary intake -End Table-I I ' : __ '. APPENDIX A SCIENTIFIC AND COMMON NAME 1 OF FISH COLLECTED IN THE NEW CUMBERLAND POOL OF THE OHIO RIVER, 1970 THROUGH 2016 BVPS 1 Nomenclature follows Robins, et al. (1991)

' I I Appendix A SCIENTIFIC AND COMMON NAME 1 OF FISH COLLECTED IN THE NEW CUMBERLAND POOL OF THE OHIO RIVER, 1970 THROUGH 2016 BVPS Family and Scientific Name Common Name Lepisosteidae (gars) Lepisosteus osseus Longnose gar Hiodontidae (mooneyes)

Hiodon alosoides Goldeye H. tergisus Moon eye Clupeidae (herrings)

Alosa chrysochloris Skipjack herring A.pseudoharengus Alewife Dorosoma cepedianum Gizzard shad Cyprinidae (carps and minnows) Campostoma anomalum Central stoneroller Carassius auratus Goldfish Ctenopharyngodon idel/a Grass carp Notropis spilopterus Spotfin shiner Cyprinus carpio Common carp C. carpio x C. auratus Carp-goldfish hybrid Luxilus chrysocepha/us Striped shiner Macrhybopsis storeriana Silver chub Nocomis micropogon River chub Notemigonus crysoleucas Golden shiner Notropis atherinoides Emerald shiner N. buccatus Silverjaw minnow N. hudsonius Spottail shiner N. rubel/us Rosyface shiner N. stramineus Sand shiner N. volucellus Mimic shiner Pimephales notatus Bluntnose minnow P. promelas Fathead minnow Rhinichthys atratulus Blacknose dace Semotilus atromaculatus Creek chub Catostomidae (suckers)

Carpiodes carpio River carpsucker C. cyprinus Quill back C. ve/ifer Highfin carpsucker Catostomus commersonii White sucker Hypente/ium nigricans Northern hogsucker lctiobus buba/us Smallmouth buffalo I. niget Black buffalo Minytrema melanops Spotted sucker Page 1 of3 Family and Scientific Name Moxostoma anisurum M. carinatum M. duquesnei M. erythrurum M. macrolepidotum lctaluridae (bullhead catfishes)

Ameiurus catus A. furcatus A. me/as A. natalis A. nebu/osus lctalurus punctatus Noturus f/avus Pylodictis olivaris Esocidae (pikes) Esox lucius E. masquinongy E. lucius x E. masquinongy Salmonidae (trouts) Oncorhynchus mykiss Percopsidae (trout-perches)

Percopsis omiscomaycus Cyprinodontidae (killifishes)

Fundu/us diaphanus Atherinidae (silversides)

Labidesthes siccu/us Percichthyidae (temperate basses) Marone chrysops M. saxatilis M. saxatilis x M. chrysops Centrarchidae (sunfishes)

Amblop/ites rupestris Lepomis cyanellus L. gibbosus L. macrochirus L. micro/ophus L. gibbosus x L. micro/ophus . Appendix A (Continued)

Common Name Silver redhorse River redhorse Black redhorse Golden redhorse Shorthead redhorse White catfish Blue catfish Black bullhead Yellow bullhead Brown bullhead Channel catfish Stonecat Flathead catfish Northern pike Muskellunge Tiger muskellunge Rainbow trout Trout-perch Banded killifish Brook silverside White bass Striped bass Striped bass hybrid Rock bass Green sunfish Pumpkinseed Bluegill Redearsunfish Pumpkinseed-redear sunfish hybrid Page 2 of3 I I __ Family and Scientific Name Micropterus dolomieu M. punctulatus M. sa/moides Pomoxis annularis P. nigromaculatus Percidae (perches)

Etheostoma blennioides E. nigrum E. zonale Perea flavescens Percina caprodes P. copelandi Sandercanadense S. vitreum S. canadense x S. vitreum Sciaenidae (drums) Aplodinotus grunniens 1 Nomenclature follows Robins, et al. (1991) Appendix A (Continued)

Common Name Smallmouth bass Spotted bass Largemouth bass White crappie Black crappie Greenside darter Johnny darter Banded darter Yellow perch Log perch Channel darter Sauger Walleye Saugeye Freshwater drum Page 3 of3