ML19308D994

Proposed Plankton & Water Quality Programs at Facility

ML19308D994
Person / Time
Site:	Crystal River
Issue date:	07/31/1973
From:	Maturo F FLORIDA POWER CORP.
To:
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ML19308D991	List: ML19308D990 ML19308D994
References
NUDOCS 8003200731
Download: ML19308D994 (39)

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g FLORIDA POWER CORPORATION Proposed Plankton and Water Quality Programs At Crystal River ZOOPLANKTON SAMPLIhG This program is in a state of flux. We have contracted to hire a team of sixteen under Dr. Maturo to address this problem (enclosure 1). Initially, extensive sampling will be conducted in the area of station E, the intake screens (see attached map of sampling areas). Variability will be established and used 1

1 to determine the number of replicates necessary for a given confidence level.

These figures will be confirmed by Dr. Lackey, the consulting biometrician, whose resume is included as enclosure 3 to this document.

f Based on the sample variability as determined above, one of three paths will

- t be taken in further research. If a small number of replicates will give reasonable results, a statistically valid sampling of all stations, as our'_ned in enclosure 1 will be implemented. If a fairly large number of samples is required to achieve reliable data, the program will be limited to the immediate environs of the canal system and discharge canal, stations E, F C and H.

Finally, if the number of replicates is prohibitively high, (i.e.) beyond the capability of the available manpower resources to process, a characterization of the area similar 4

to that outlined in enclosure 1, but not having a high degree of statistical confidence will be undertaken.

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ZOOPLANKTON FATE AND CONDITION The program outlined in enclosure C of the document ent,itled " Florida Power Corporation Crystal River Environmental Research Program to Meet Current Federal Requirements June 11, 1973" is being implemented.

PHYTOPLANKTON Phytoplankton Characterization will be conducted by Mr. Robert Gibson under the supervision of Dr. T. L. Hopkins of the University of South Florida. A copy of this proposal and Mr. Gibson's Resume can be found in enclosure 2.

WATER QUALITY Water Quality Samples will be routinely taken during the biweekly sampling program outlined in enclosure 1.

When possible, additional samples will be analyzed to correlate with other phases of the research effort at Crystal River.

It is expected that the following perimeters can be routinely measured:

Total Nitrogen Orthophosphate Total Dissolved Organic Carbon Silicate Total Phosphorus Salinity Nitrate Dissolved Oxygen Nitrite Temperature Ammonia Current Speed & Direction

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EIiCLOSURE 1 4

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RESEARCH PROPOSAL TO FLORIDA POWER CORPORATION TITLE: Effect of Power Plant Operation on Sha13cw Water Coastal Zooplankton PRINCIPAL INVESTICATOR: Frank J. S. Maturo, Jr.

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ADDRESS: Zoology Department, University of Florida, Gainesville, Florida 32611 DURATION OF PROJECT: 15 months (August 1,1973 - November 30, 1974)

STARTING DATE: Auguse 1, 1973 INTRODUCTION There is a primary need for base line information on the effects of coastal power plant operation on the biota of the adjacent areas. Not only are organisms subjected to a modified environment resulting from the ' thermal plume produced by power plants, but some forms such as zooplankton and fish may be entrained in the cooling waters and exposed to the maximu; thermal and mechanical shock when forced through the heat exchanger system.

Zooplankton is of major concern because its components include the major food chain organisms in the marine environment as well as the larvae and juveniles of commercially important species. The purpose of this proposal is to estimate the effect of Florida Power Corporation's Crystal l

River power plants (Nos.1, 2, and 3) on the zooplankton in the immediate l

l area. The study will employ biweekly, monthly, and quarterly sampling programs in both intake and discharge areas. Both physical and biological data will be taken for multivariate and other statistical analyses.

l OBJECTIVES The approaches to be used to arrive at answers to the major question l

l are as follows. We will:

• (1) estimate power plant " predation" on zooplankton; gt t

's (2) estimate the efficiency of the plant as a predator; (3) estimate zooplankton standing crop in intake and discharge areas; (4) ' estimate standing crop of natural zooplankton predators (ctenophores, chaetognaths, fish larvae, etc.);

(S) estimate zooplankton production and compare discharge with intake area; (6) monitor species diversity as a stress indicator.

These approaches will be implemented through intensive field and limited laboratory studies. Due to inherent sampling difficulties under unusual, adverse weather conditions, sampling will monitor only normal seasonal weather conditions.

TECHNIQUES TO ACCOMPLISH OBJECTIVES

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ni Nine stations will be established according to the plan in Figure 1.

The physical features of'the coastal region in the vicinity of the plant site permit a division into 3 areas. Area 1 includes a large shallow area containing numerous oyster bars running roughly parallel to the shore, its western boundary 1 eing the last Gulf-ward string of these bars. Area

2. includes all of the water from the west boundary of Area 1 to an imaginary line drawn north and south through the physical end of the north intake spoil bank. Area 3 is west of Area 2 and otherwise unbounded into the Gulf of Mexico.

The north dike of the intake canal physically divides Areas 1 and 2 into tmsubdivisions which will be designated " intake" and

" discharge" for each. According to very preliminary hydrographic studies by Dr. K. Carder (Technical Rept. #2,1973), entrained water is drawn mostly from Areas 2 & 3 intake sides. His data "suggest that during flood [ tide] the intake water is primarily of gulf shelf origin, and during N,

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ebb it contains only 10% more water of river origin'." He states that water of frea 1 (intake ) is retarded in its access to the plant intake channel by high (shoal water) friction and oyster reef barricades.

Station A will be located inshore south of the intake canal midway between the coastal marsh and the canal opening, a distance of 1.2 nautical f

miles offshore. The substrate in this area is sand and shell between prominent oyster bars.

The depth is 4 ft. at MLW. The salinity is noticedbly affected by Crystal River effluent.

Station A should give a measure of inshore zooplankton features.

Station B will be southwest of the intake canal opening in an area (2) which is considered to be part of the source of the entrained water.

The depth is about 7 f t.

at MLW; the substrate is hard sand.

Station C, six miles offshore, will be located beyond the end of the unbroken northern side of the intake canal.

The depth is about 15 ft.

in the channel and 9 ft. outside it (south) at MLW.

The substrate app. ors to be hard sand.

This station should represent characteristics of the major portion of entrained water.

Station D will be located in the mouth of the intake canal. Comparisions between it and the next station should give some indications of zooplankton generated in the canal itself.

Station E will be located just in front of the intake screens of~

Units 1 and 2.

The depth is 15 ft. at MLW. The substrate appears.to be,.

a fine coal dust sediment.

All water here will pass through the condensers.

Station F will be in the immediate area of the condenser outfall. Most of the zooplankton here can be expected to have passed through the plant.

Station G will be positioned in the channel at the end of the north dike of the discharge canal.

Zooplankton characteristics observed here l

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plant " predation" effects and hopefully give some measure of eddy 1

recruitment in the discharge canal.

Station H will be in the channel at the end of the south dike of 1

the discharge canal. Data from here should characterize zooplankton of

. the thermal plume.

i Station I will be located in the discharge side of Area 2.

It should i

provide data for comparison primarily with Stations B and C for standing 1

j crop estimates.

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Objectives 1, 3, 4, and 6 will be accomplished through the sampling

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program displayed in Table 1.

Sampling activity will have three components:

l biweekly (fortnightly), monthly, and quarterly.

Collecting gear proposed for this study are 1) portable pumping systems containing in series a etenophore trap, a 202u mesh filter net, and 64u mesh filter net (quarterly only) and 2) a 1 meter diameter, 505u mesh, 5:1 length / width ratio, net for collection of fish eggs and larvae. The pumping systems will be of our own design based on a device described by Icanberry and Richardson (L & 0, March 1973). Pumping rate will be 30 gallons per minute maximum. The total volumes to be sampled each time have yet to be determined.

The Biweekly Sampling Program will involve the 202u mesh filter nets and the 1 meter, 505 u mesh nets. Pumps will sample surface waters only except at Stations E (plant intake) and G (end of north discharge dike).

Station E will be examined at 3 depths: surface, mid-depth, and bottom.

Station G will be sampled at 2 depths: surface and bottom.

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will be used for surface tows only; it will be omitted at Stations A and H because the water is too shallow. Meter nets will be towed for 10 minutes.

Three replicate samples will be made at each pump sample location (depth).

All samples will be processed for biomass. One sample will have identifications ee'S me e.

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(ID) and counts made in the following categories:

Acartia tonsa and 4 other commonest copepo'.s d

1 Calanoid copepods i

Harpacticoid copepods Cyclopoid copepods Gastropod veligers Bivalve'veligers Barnacle larvae Penacid shrimp Other shrimp Crab larvae Other crustaceans i

Polychaetes Echinoderms Chaetognaths Tunicates Medusae i

j Misec11aneous Fish eggs Fish larvae The other two replicates will have identifications made in four categories (for statisical analyses):

Calanoid copepods Bivalve veligers c-Shrimp larvae 1

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Biomass determinations made in conjunction with category identifications will follow the format of our present zooplankten contract as follows:

One half of the sanple is separated in a sieve series with standard mesh sizes Nos. 10, 20, 30, 60, and 120 (additional series included for 64u mesh samples); the remaining half sample is kept as a reserve.

Each etze class is split repeatedly until the number of organisms present is of a countable size and the water volume is approximately 60 ml.

The sample,is then placed in a 100 cm. squa e gridded dish and 5 counts covering 4 squares each are made. Thus, 20% of the aliquot is counted for each size class. We can then determine the per cent composition of each fraction by recording counts per zooplankton type divided by the total count of all zooplankton in the fraction sample (part of computer program).

Each sieve fraction is vacuum filtered onto a preweighed Whatman No. 5 filter disc, oven-dried and weighed to determine the dry weight of the fraction. Data analyses will be discussed in another section below.

The sieve separation method provides a more accurate estimation of biomass by species categories and facilitates counting procedures by sorting organisms to size and reducing the number of species per sample.

Total counts of fish eggs and fish larvae will be made from the meter net samples.

Ctenophore biomass will be determined volumetrically. Water samples

-1 will be taken at each station for nutrients,f detritus, and.phytoplankton analysis, i

These samples will be processed by other agencies to be arranged for by Florida Power Corporation. Salinity, temperature (air and water), secchi dise, tide stage, sea condition, meteorological conditions, current direction and velocity, and dissolved oxygen determinations will be recorded for each station.

Stations missed because of bad weather conditions and/or i

l equipment failure will not be made up.

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Q The Monthly Sampling Program will involve a more detailed study of' Stations E and G.

During a diurnal cycle, up to five peak periods of zooplankton occurrance (as determined by the Quarterly Sampling Program) will be sas. pled. These will be surface samples taken with the 202u filter pumps.

Three replicates will be taken at each " peak period." One sample will be treated for all categories identification and biomass. The other two samples will be processed for biomass only.

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The Monthly Sampling Program is designed to provide a monthly estimate of the " highest predation levels" of the power plants.

The Quarterly Sampling Program will be used to provide correction factors for zooplankton biomass and numbers per category; both are needed because of limited mesh size and depth in the biweekly samples.

It also identifie's the peak zooplankton periods to be used in the monthly samples.

A 64u mesh filter net will be used to collect the. smaller zooplankton normally missed or underrepresented in the biweekly samples at all stations.

Two replicates will be taken at each station or depth. These samples will later be sieved through a 202u mesh ns.t in the lab and the two size classes obtained will be used as correction estimates for differences in zooplankten biomass, numbers, and size resulting from mesh employed and variation with depth.

Identifications will be made for all categories in the 64u mesh residual from Stations D, E, F, and H.

The 202u mesh components from Stations B, C, D, H, and I will be identified for all categories. Station F has no depth correction because it is the condenser discharge site where we are assuming complete mixing of the water column.

The Quarterly Sampling Program will also include a diurnal biomass

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variation study using the 202u mesh filter pump. Four replicates will be made each hour at the depths indicated in Table 1.

From these, up to 5 peak periods of zooplankton biomass will be selected for all category

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,i Table 1.

ZOOPLANKTON SAMPLING AT CRYSTAL RIVER Biweekly Monthly Quarterly 64p Net correction 202p Net depth Diurnal Biomass Biomass fdcror for correction factor with 5 Peak ID l

+ Nutrients

+4 category Peak Period biomass, size for biomass and 24 hour2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> 6 hourly interval-Station.

+ Phytoplankton ID-Biomass Diurns' and numbers numbers (ID) 202p only

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A 202 (surf) 202 (surf)

Biomass Only NA 2 fixed & 2 revolving

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I depth Stations (2 depths).

Species diversity at 1 intake and 1 discha'rge station (6 hr. interval) i B

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C 202 202 (surf)

Biomass Only 505 (8"#f) 2 depths 2 depthe j

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D 202 202 (surf) 64p 3 depths 3 depths 505 (surf)

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G 202 2 depths 202 (2 depths) 202 surf Biomass Only 2 depths 505 surf ID/ Biomass 2 depths H

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I identification.

Stations E and G will be sampled every quarter; two other stations will be selected eae.h quarter according to interest generated by j

previous processing. At least two depths w;E be sampled at each of these.

Species diversity will be, determined at 1 intake and 1 discharge station each quarter. Diversity will be calculated on 6 hour6.944444e-5 days <br />0.00167 hours <br />9.920635e-6 weeks <br />2.283e-6 months <br /> intervals based on the number of species present in the first 1000 organisms counted. These i

data will be given to Dr. H. T. Odum for use in his modelling project.

Objective 2, efficiency of the power plant as a predator, will be i

carried out by the " Effects of Power Plant Entrainment on the Major Species of Copepods" contract being conducted concurrently by Maturo and Alden.

1 Objective 5, zooplankton production in the 1itake and discharge areas, d

i will be studied in collaboration with H. McKellar (Odum contract). This i

i project will involve light-dark bottle respiration studies for which we will provide 4 man days per quarter to assist McKellar. 1he data generated I

may help to determine the effect of plant " predation" on the zooplaiikton standing crop.

The number of samples generated in each part of this proposal and the man days per month required to collect and process them are presented in Tables 2 and 3.

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Table 3.

Monthly manpower requirements for field sampling.

1) BIWEEKLY SAMPLING 3 teams x 2 men / team x 1 day 12 man-days /mo.

x 2 biweekly periods /mo.

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2) MONTHLY PEAK PERIODS 16 man-days /mo.

men / shift x 4 shifts x 2 stations

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3) QUARTERLY 64p AND 202p Vill be included in regular biweekly sampling program.

4) QUARTERLY DIURNAL 1 team x 2 men / team x 4 shifts x 4 stations = 32 man-days /qtr. = 32/3 11 man-days /mo.

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5) SPECIES DIVERSITY No additional field work; will be collected during quarterly diurnals.

6) ZOOPLANKTON PRODUCTION

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2 men x 2 days / quarter = 4 man-days /qtr. = 4/3 2 man-days /mo.

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TOTAL 41 man-days /mo.

+ 20 workdays = 3 perac e

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DATA ANALYSIS Data will be recieved and transcribed onto coding sheets.

These sheets will then be fed into an optical scanner which will create an 800 bpi 9-track data tape. The data tape will then be listed and checked for coding errors. Corrections, if necessary, will be made and an updated complete data tape will be created. Back up tapes will be created as an insurance against data loss.

Prior to anal-sis a'1 count and biomass data will be converted to a 1

3 single volumetric base (biomass or numbers /m ).. Those samples to be used in statistical analysis will be subjected to normalizing transformations if it is deemed necessary (e.g. the log transformation on count data).

Where sample size is adequate to allow an estimate of variance, statistical analysis will be performed. Biomass and selected categories' numbers will be analyzed using multivariate analysis of covariance (MANCOVA) (Morrison, 1967; Cooley.& Lohnes, 1971).

The covariate will be a set of variables describing the physical environment of the station and the power fluctuations of the plant. This will investigate the problem of differences between stations and whether these differences are environmentally related, plant related, or intrinsic to the station. Using a multivariate study will combine investigations of raw numbers (e total biomass) and diversity

(= coneunity composition). Data will be compared within and between comparable time periods.

The above data in conjunction 'with the samples taken as single sample 7

estimates will be used in a descriptive sense to characterize the zooplankton connunity in the vicinity of the plant. Output examples of this t rpe include :

1) total number of zooplankters/m3; 2) numbers of different kinds. of zooplankters/m ;

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3) numbers of zooplankters of a specific size range /m3; 4) % composition of each i

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I animal type in the plankton community; 5) % composition of each animal type 3

p in a specific size range; 6) total zooplankton biomass /m ; 7) biomass of each l

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animal category /m ; 8) biomass of a specific size range of zooplankter/m ;

9) weight in pounds and grams of zooplankton that are entrained by the plant 1

at the intake site for the present operation (Units 1 and 2); and 10) projected 1

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entrainment potential for future operation (Units 1, 2, and 3).

Output on i

number 9 and 10 abov2 will be in 3 time units: per minute, per hour, per day.

The results of the analysis will be presented in the manner'that provides

• harts, tables, graphs the quickest and easiest assessment of its meaning:

c (2 & 3 dimensional), and mapping techniques will all be used to their best advantage.

1 The Statistical Analysis System (SAS), Statistical Package for the Social Sciences (SPSS), UCLA Biomedical Series (BMD), IBM Scientific Subroutine (SSP),

i the mapping routine (SYMAP), as well as various packages and subroutines indigenous to the North East Regional Data' Center at the U. of F. (NERDC) 1

[e.g. tape utilities and Calcomp plotter and printer plotter packages) will be used at various steps in the analysis. In addition several~ data handling programs will need to be written in order to generate drea sets from the raw

~ dat'a*and as main routines for preparing the final output. As much as possible of the final results to be used in reports will be output via computer.

The data analysis aspect of the project requires the following personnel i

and equipment:

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m MISCELLANEOUS In addition to the boat provided for in this proposal, Florida Power Corporation will provide for our use two 17'3" AMF - Robalo Series 180 boats (or equivalents). These boats will be provided with running lights, spray hood and suntop with windshield connector and side curtains. These accessories are needed for personnel protection during adverse weather operations and extended on-board activity. One such equipped boat will also be used by the Maturo-Alden " Copepod Fate" contract.

Florida Power Corporation will also provide:

a) gas and oil for all boats; b) covered storage for boats, trailers,15 foot plankton nets, sample bottles (phytoplankton, nutrients)s freshwater line and hose for washing down boats and gear; c) lounge and dormitory facilities for contract personnel during diurnal sampling periods.

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O CURRICULUM VITAE NAME: William Ingram III BIRTH DATE: 09/02/45 BIRTH PLACE: Washington D. C.

ADDRESS: 288 Florida State Museum. Univ. of Fir.., Gainesville, Fla.

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MARITAL STATUS: Married WIFE'S NAME: Janice Ann CHILDREN: one CHILD'S NAME: Kimberly Ann MILITARY STATUS: lY SOCIAL SECURITY NUMBER: 577-60-9443 j

HICH SCHOOL: Yorktown Sr. High School, Arlington, Virginis i

GRADUATION DATE: June 1973 i

COLLEGE:

i Bachelors: Brigham Young Univ. ; Provo, Utah Sept.1963 - Jan.1964 George Washington Univ. ; Wash. D. C.

Jan. 1964 - May 1966 Sept. 1966 - June 19'68 Brigham Young Univ.

Degree: BS Major: Zoology Minor:

Chemis'try l

Masters:

Brigham Young Univ.

Sept. 1968 - June 1970 Degree: MS Major: Zoology Minor: Statis tics PhD:

Univ. of Florida; Gainesville, Fla.

Sept. 1970 - present Degree: not awarded yet

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PROFESSIONAL SOCIETIES: Beta Beta Beta, Biological Honorary Fraternity I

Brigham Young Univ.

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RELEVANT COURSE WORK:

Statistical Analysis for Research Workers 15 gtr. hrs.

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Advanced Statistical Analysis 5

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J Fortron Computer Programming 5

2 Advanced Computer Methods in Statistics 5

d Mathematical Statistics 9

I Multivariate Data Analysis 4

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~s Mathematical Ecology (Special Topics in Statistics) 4 gtr. hrs.

Methods in Mathematical Ecology (Special Problems in Industrial & Systems Engineering) 5 Calculus and Differential Equations 23 Linear Algebra

_3, 78 str. hrs.

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ENCLOSURE 2 f

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....7 INTRODUCTION This proposal seeks funds for an investigation of phytoplankton populations in waters adjacent to Florida Power Corporation generating plant at Crystal River, Florida. The' project was conceived to obtain base line data in the vicinity of an operating power plant which anticipates an increased cooling water requirement. At present two 897 MW organic fuel units are in operation at Crystal River and a nuclear unit will go on line in 1974. This investigation vill assay present impact on phyto-plankton of the units currently in operation and vill provide data for estimation of the impact of increased power generation (therefore additional cooling water requirements) at this sit?..

This information vill also provide a basis for an evaluation of the impact on phytoplankton in waters adjacent to another Florida Power Corporation power plant presently under construction at the mouth of th'e Anclote River, Florida.

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OMmm The broad objective of this research is to investigate phytoplankton populations in the vicinity of the Florida Power Corporation generating plant at Crystal River. The specific goals are listed below:

1.

Obtain.a seasonal picture of phytoplankton species abundance, biomass, and production at stations located throughout the area adjacent to the Crystal River plant.

2.

Estimation of power plant impact on entrained phytoplankton through comparisons of populations in both the intake and, discharge canals.

These goals may be modified as experimental results indicate the need for such changes.

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y METHODS Water sa:nples for phytoplankton analysis vill be collected from 9 stations in the study area (see Figure 1) by University of Florida personnel. These samples, preserved in isolution of mercuric chloride or a solution of formalin, will be brought to the Department of Marine Science Laboratory in St. Petersburg'for study by University of South Plorida technicians. Phytoplankton in each water sample vill be con-centrated by the Dodson tube settling method (Dodson & Thomas, 1964) and' counts vill be made on aliquots of the sample concentrate using modifications of the Utermohl inverted microscope technique (Utermohl, 1931). Tape recorders will be employed to reduce time and effort of

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recording phytoplankton counts. The recorded counts will be subsequently transcribed onto data sheets with a format, suitable for statistical analyses (correlation. statistics) by computer.

Phytoplankton production measurements will be made monthly with 1hC-uptake methods currently in use on the Florida Power Corporation Anclote Project. Thesc. techniques are described in the 1972 Anclote Project Annual Report (Baird,e_t.al, 1973) and are modifications of those t

described in papers by Lind and Campbell (1969), Pugh (1970), and Schindler and.Holmgren (1971). Primary production measurements will be l

made at each of the nine stations using surface water and a deck incubator.

Samples inoculated with NaH1D CO3 vill be incubated for a period of 6 hours6.944444e-5 days <br />0.00167 hours <br />9.920635e-6 weeks <br />2.283e-6 months <br />.

' Incubator values will be periodically checked with g situ primary pro-duction experimentn conducted at selected stations.

The field work to k

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implement the productivity portion of the program vill require up to 3

' days each month, depending on weather conditions. Boats,. motors, and.

housing vill be provided at the Crystal River plant by Florida Power Corporation.

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RzFERENCES

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Dohon, A.N. and W.H. Thomas. 1964. Concentrating plankton in a gentle o

fashion. Limnol. Oceanogr., 9: 455-456.

Lind, 0.T. and R.S. Campbell'. 1969.' Comments on the use of liquid scintillation for routine determination of 1hc activity in production studies.

Limnol. Oceanogr., 14: 787-789 1k0 diatom material Pugh, P.R. 1970. Liquid scintillation counting of on filter papers for use in productivity studies. Limnol. Oceanogr.,

15: 652-655 Schindler, D.W. and S.K. Holmgren. 1971. Primary production and phyto-plankton in the experimental lakes area, Northwestern Ontario and other low-carbonate waters and a liqtiid scintillation method for 1kC activity in photosynthesis. J. Fish. Res. Bd.

determining Canada, 28: 189-201.

Utermohl, H. 1931. Neue Wege in der quantitativen Erfassung des Planktons.

Verh. int. Ver. Limnol., 567-596.

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i M0!!TIILY PERSONIIEL TI!E ALLOTMENT

1. marine biologist III hours / month field time 2h t

lab time H6 data analysis

'20 160

2. marine-biologist II field time 2h lab time H6 data reduction 12 data analvsis 8

160

3. data reduction / clerical da,ta reduction 24 typing / computer 128 administrative.

8 1@

h. graduate research assistan';

field time 48 lab time 16_

data analysis 16 80

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Note: This represents projected time allotment ~and is subject to change as the program progresses for more efficient time utilization.

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CAPITAL EQUIR!ENT 1.

A " Zeiss" inverted microscope is a necessary addition to a simila:,

microscope currently being used on the Florida Power Corporation Anelote Projec.t in order to analyze the samples within the agreed-upon time schedule for data reporting.

2.

A 6-place " Mil 11 pore" filtering manifold will speed the 1kC-primary production filtering and thereby reduce error caused by ex::essive filtering times. In addition this apparatus would prevent interference with the extensive primary production. program at Anclote.

3 A "Brinkman" basic pipettor has proven vital to the lh C innoculation procedure at Anclote. Thus to prevent interference with the Anclote.

program we are requesting a second unit.

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A "Millipore" vacuum pump is also required to minimize interference with the Anclote program.

5 A vaccine seal crimper will replace the vaccine seals presently in use

..which cannot be locked onto the lhC stock solution bottles. This solution has to be autoclaved prior to storage and use. During auto-claving some of the unlocked seals unseat and require careful reauto-claving as well as loss of the solution. The aluminum crimp which locks these seals onto the bottle require this partictilar crimping

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N, device 6.

Tape recorders decrease phytoplankton microscope counting time and i

error. Thus two cassette tape recorders, one for each technician counting vith the microscopes, are in the budget.

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Othercapitalequipmentitemstobeusedonthispro[ectarepresently available at the Department of Marine Science, USF. These items are:

(1.) salinometer (2.) submersible pump O

(3.) 125ml BOD bottles (b.) truck (S.) spectrophotometer ("Beckman" or "Perkin-Elmer/Hitachi")

(6.) " Zeiss" inverted microscope with integral automatic 35mm camera system.

It is. understood that a FPC boat and motor vill.be provided at-Crystal River for field work.

Title to all equipment purchased under this contract is to be vested in the University of South Florida.

PAYMENT SCHEDULE 1

Payments are to be made quarterly, upon receipt of invoices prepared l

by the University of South Florida and submitted to Florida Power Corporation, l

each in the amount of one-fourth (1/h) of the total contract, beginning August 1, 1973.

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a ucau:ne Robe'rt J.. GibLan date of birth: 24 October 1946 place of birth: Birmingham, Alabama

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marital status: wife, Donna C. Gibson, no children present home address:,5020 D Beach Dr.

S.E., ST. Petersburg, Fla. 33705 present occupation: student and research assistant, Univ.

of South Florida education:

schooY degree date West End High School H.S. diploma 1964 Univ.'of Alabama B.S.

1969 Univ. of Alabama M.S.

1970 i

teaching experience:

school course capacity semestors U. of Alabama Limnology lab instructor 2

Biology I 1

C5il Biology " lab assistant 1

Masters thesis: " Changes in Plankton Community Structure A'ssociated with the Impouddment of the North River, Alabama", abstract in The ASB Bulletin, vol. 17, No. 2, p. 44.

research experience:

1. fresh water plankton' counting and identifi-cation; (Masters thesis research);

2. nearshore estuarine nutrient water chemistry analysis; (USF Anclote Proj. 1970-present) ;

3. nearshore estuarine phytoplankton production, enumeration, and identification; (USF ANclote

- Proj. 1970-present) ;

4. chlorophyll determination aboard NRL/USNS Mizar Cruise 71-ll-02B, 1-15 June 1971;

5. productivity /C 4 measurement abord SUS-7208, l

B-7208 R/V BELLOWS, 1-10 August 1972;

'6. Sargasdum productivity / offshore Anclote nutrients s

aboard SUS-7215, B-7212 R/V BELLOWS, 16-20 October 1972.

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7. R/V Bello 5 r mine MS-73-11, F rt:onncun B Wetivity w.llitary; ILT UJAR, 3 y ear 'U3Ali co:utitmud..

profoscicnal ce bcentipa:

American A.wociatica or Phycole;*y:

Americ in' Association of Limnolocy and Oceanog:aphy; Marine Biological A0sociation of the United Kingdom; Association of Soui;heestern Biologists Plankton Society of Japan Phi Sigma Society publications:

Gibson, Robert A. & Thomas L. Hopkins. 1973. C. Water Quality, II.

Phytoplunkton. In Baird et al. 1973. Anclote Enviremental Project Report 1972. Contrib. No. 41; Dept. cf Marine Science, USF. 220p.

'Hopkins, T.L., R. Gibson, W. Gunn, J. Johnson, V. Mcynard, & V. Weiss.

1972. C.1.'ater Quality. In Baird et al.1972. Anclote Enviromental Project acport 1971. Contrib. IIo.39; Dept. of Marine Science, USF, 251p.

Blake, N.J., L.J. Doyle, T.E. Pyle, R.M. Crosby, J.L. Feigl, R.G. Garza, R.A. Gibson, R. Johansson, V. Maynard, D.W.Wallace, J.A. Watson.

and R. Zimmerman. 1973. A Field Study of Selected Ecological Properties of Boca Ciega Bay and Adjacent Gulf of Mexico. Geo-Marino, Inc. Report to Bd. of Gy. Comm., Pinellas Cty. F1.; 121p Blake, N.J., T.E. Pyle, L.J. Doyle, D.W. Wallace, R.K. Klausewitz, R.A.-

Gibson, and R.J. Zimmermail. 1973. Biological and Physical Mea-surements in the Vicinity of a Proposed Advanced Waste Treatment Outfall, Anclote Isiver, Florida. Geo-Marine. Report to Ed. of Cty.

Comm., Pinellas Cty., F1. 83p.

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.s Suram2.y of course work at the University ot Alsbaua 1964-196'f con v se d en<':v.i x inr.

ner.-ter $cazs undergraduate (Biol ogy) major totany 4

Modlogy 4

Biological Conservation 2

, Morphology of the Lower Plants 2

. Introductory Genetics 2

Field Zoology 4

Vertebrate Comparative Anatomy 4

History of Biology 2

Cell Physiology 4

Lir.nology 4

Invertebrate Biology 4

Introduct'ory Microbiology 4

Biology of Marine Vertebrates 6-Marine Science Seminar 4

undergraduate minor (Geology)

Earth Science I 4

Earth Science II 4*

Coastal klain Geology 3

Palececology 3

Geological Oceanography 6

other Math 12

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Physics 8

chemistry 13 Histiory 6

Anthropology, 6

Latin 6

English 12 i

Army ROTC 16 graduate major (Biology)

Ecology 4

4 Endocrinology 3

Invertebrate morphology 2

Phycology 4

Ichthyology,

3 Graduate Seminar 6

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LAW ENGINEBUNG TESTING COMPANY Geotectwvcal aM Materiais Ergineers 2T49 DCLK RDAD. S E.' MAR!ETTA GEORGIA 3:052 / 404) 9719305 t

July 19,1973

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Florida Power Corporation Environmental Affairs P. O. Box 14042 320134tn Street South St. Petersburg, Florida Attention: Hessrs. Ken Garrison and Joe Johnson

Reference:

Selection of biostatistical consultant for statistical control functions relative to Florida Power Corooration Crystal P.iver Environmental Researco Prooram to meet Current Federal Reauirenents.

June 11. 1973

~

Gentlemen:

In response to your request on June 20, 1973 Law Engineering Testing Company has located and retained the above referenced consultant to advise on statistical design of various phases of the envimnaental researen program presently underway and/or planned at Crystal. River, Florida.

In our efforts to do so, we established the following ninimum criteria andqualifications for the individual that would be cnosen to serve in tnis capacity:

1, Adequately trained in at least one or more areas of aquatic ecology that are relevant to the efforts of Florida Power Corporation at Crystal River, Florida.

This level of training was considered to be the doctoral level. In addition, this' training or specialty area should extend deeply into or be enhanced strongly by statistical training. This training level was con-sidered to be a minor in statistics at the doctoral level.

2.

Indicate continued professional development and compe-tence -in those specialty areas of training relevant to the Florida Power Corporation Crystal River Program 4

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Florida Power Corp rcticn 1.

July 19, 1973

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Page Two through the medium of technical publications in reputable scientific journals in the recent past.

3.

Direct experience in applied environmental programs.

Law Engineering Testing Company has retained Dr. Rotart Lackey of the Department of Fisheries, Virginia Polytechnic Institute, Blacksburg, Virginia. Dr. Lackey's qualifications exceeded the established criteria for selection. I have had several personal conversations witn Dr. Lackey in an effort to more thoroughly explore his tecnnical backgrouad and the relevance thereof. I have also obtained a personal recomendation on Dr. Lackey from a close prcfessional collegue of mine, Dr. Kenneth Dickson, Assistant Director Institute for Environmental Studies, Virginia Polytechnic Institute, Blacksburg, Virginia, with whom Dr. Lackey has worked recently.

A personal resume has been obtained which describes Dr. Lackey's educational, professional and technical background. A copy of this has been famarded to the Environmental Affairs Department of Florida Pcwar Corporation for your use.

I have' asked Dr. Lackey to forward to Law Engineering copies of all pertinent technical publications. As soon as I receive these, I will famard copies to Florida Power Corporation.

If we can be of further assistance in regard to this comunication please feel free to contact us directly.

.Very truly Jours.

LAW ENGINEERING TESTING COWANY nd$

b y Don E. Henley, Pir.D.

Manager Environmental Science Services DEH:js 4

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April 2,1973 3

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_ ROBERT T. l.ACKEY Assistant Pmfessor, Fisheries Science Iepartment of Fisherfes and Wildlife Sciences Virginia Polytechnic Institute and State University Blacksburg, Virginia 24061 3

(703)951-6944 Education BS. Fisheries Science, Califomia State University. Humboldt,1967 MS. Zoology, University of Maine.1968 PhD, Fisheries Science (Statistics Minor), Colorado State University,1971 A

Research Interests Fisheries management. impulation dynamics, aquatic ecology Research and Field Exoerience Assistant Professor, Fisheries Science. V.P.T. 8 S.U.,1971-present J

Research Fellow, colorado State University, 1968-1971 Research Assistant. University of Maine, 1967-1968 Fisheries Assistant, California State University, Hurtoldt, 1965-1967 Fishertes Aide. Alaska Department of Fish and Game,1965-l%6 Fisheries Alde. Califomia Department of Fish and Gane,1964

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d Teachino Experience k

FW 3020 Introduction to Fisheries Science (Fall,1971; Fall,1972)

FW S25 Fisheries Theory (Winter,1972; Winter,1973)

Pmfessional Affiliation American Fisheries Society (Certified Fisheries Scientir,t)

American Society of Lirmology and Oceanography

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Fisheries Society of the British Isles The Wildlife Society l

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. Technical Articles

• 1.

Vertical pill nets for studying depth distribution of small fish.

1968. Trans. Amer. Fish. Soc: 97(3):296-299.

2.

Food interrelationships of salmon, trout, alewives, and snelt in a Maine lake. 1969. Trans. Amer. Fish. Soc. 98(4):641-646.

3.

Observations on newly introduced landlocked alewives in Maine.

1970.

N.Y. Fish and Game Journal. 17(2):110-116.

4.

Seasonal depth distributions of landlocked Atlantic salmon, brook tmut, landlocked alewives, and tnerican smelt in a small lake.

1970.

J. Fish. R.-s. Bd. Canada. 27(9):1656-1661.

5.

Use of sugar flotation and dye to sort benthic sarples. 1971.

Trans. Amer. Fish. Soc.

100(4):794-797.

(With Bruce E. May) 6.

A technique for climinating thermal stratification in lakes.

1972. Water Resources Bulletin, J. Amer. Water Resources Assoc.

8(1):46-49.

7.

Evaluation of two methods of aeration to prevent winterkill. 1972.

Prog. Fish-Culturist. 34(3):175-178.

(With Donald W. Holmes) 8.

Response of physical and chemical parameters to eliminating themal stratification in a reservoir. 1972. Water Resources Bulletin, J.

Amer. Water Pesources Assoc. 8(3):589-599.

9.

Evaluation of diel variation in androcen levels of rainbow trout, Salmo ca_frdneri.

1972. Copeia. 72(4):865-868.

(With Carl. B.

Schreck and M. Lloyd Hopwood)

10. Effects of artificial destratification on zooplankton in Parvin Lake. Colorado. Trans. Amer. Fish. Soc. 102(2):450-452.

11. Artificial reservoir destratification effects on phytoplankton.

1973.

J. Water Pollution Control Federation. 45:(In Press).

12. Plasma oestrogen levels in rainbow trout Salno gairdneri Richardson.

1973.

J. Fish Biology. 5(1):(In Press).Tth' Carl R. Schreck and

~

~

M.LloydHopwood)

13. Coquter assisted instruction in natural resource manaaement.

1973.

Proc. Southeastern Assoc. Game and Fish Connissioners. 26:(In Press).

(With Franklin B. Titlow)

14. Bottom fauna chances durina artificial reservoir destratification.

1973. Water Research (J. Intemational Association of Water Pollution Research) 7:(InPress).

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15. Potential of channel catfish production in Virginia. 1973.

Virginia J. Science. 24:(In Press).

(With Vaughn M. Douglass) i

16. Cage culture of channel catfish in Virginia.

1973.

J. Elisha Mitchell Scientific Soc.

(Submitted).

(With Donald H. Holmes and Henry 5. Ptsby)

17. Innovative teaching in fisheries science. 1973. Trans. Amer. Fish.

Soc.102(3):(InPress),

18. Pond culture of channel catfish in Virginia. 1973.

J. Tenn. Acad.

Science. (Submitted).

(With Donald W. Holpes and Vaughn N. Douglass)

19. Catfish farnino ir. Virginia.

(With Vaughn M. Douglass) ginia Agricultural Economics.

1973. Vir (Submitted).

20 Effect of rate of water discharge on phytoplankton in Claytor Lake.

Virginia. 1973. Proc, Southeastem Assoc. Game and Fish Comissioners.

(Suomitted).

(WithThomasL.Schulte)

Book 1.

Introduction to fisheries science. 1972. Text for FW 3020. Department of Fisheries and Wildlife Sciences, VPI & SU. Mimeo.

176 pp.

Presented Papers 1.

Shaping the aquatic environment to man's needs. Sixth Annual Short Course in Game and Fish Management, Colorado Stai;e University. Fort Collins. Colorado, Feb. 9-13,1970 2.

Lake destratification as a fisheries management tool, fifteenth Annual Sumer Conference. The Wildlife society, Central Mountain and Plains Section, Pingree Park, Colorado. August 16-19,1970.

3.

The new fisheries manaoement. Seventh Annual Western Students Wildlife Conclave, Colorado State University, Fort Collins, Colorado.

April 1-3,1971 4.

Effects of artificial destratification on a lake ecosystem. Sixth Annual Meeting, Colorado-Wyoming Chapter, American Fisheries Society, Laramie, Wyoming, April 22-23,1971, b.

Phytoplankton dynamics during artificial destratification of a montane lake. Forty-Second Annual Meetino, Colorado Academy of Science, Southern Colorado State College, Pueblo. Colorado, April 30-May 1,1971.

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9 Robert T. Lacty

6.. Effects of artificial destratification on a lake ecosystem. One Hundred and First Annual Meeting. American fisheries Society, Salt Lake City. Utah. Septenber 16-18. 1971.

7.

Faming channel catfish in Virginia. Virginia Academy of Science.

Lexington. May 4-5, 1972. Abstract in Virginia Journal of Science.

23(3):110. (With Vaughn ti. Douglass) 8.

The use of computers to teach fisheries science. Virginia Academy 4-5. 1972. Abstract in Virginia Journai of Science. Lexington. May(With Franklin 8. Titlow) of Science. 23(3):118.

9.

Winter mortality of the landlocked alewife. Alosa pseudoharengus, in Claytor Lake. Virginia. Virginia Academy of Science, Lexington, May A-5, 1972. Abstract in Virginia Journal of Science. 23(3):109.

j (With John L. Boaze) w

10. Effect of fluctuating reservoir discharge on phytoplankton population in Claytor Lake, Virgir.ia. Virginia Academy of Science, Lexington, May 4-5, 1972. Abstract in Virginia Journal of science. 23(3):117.

(With Thomas L. Schulte)

N.

11. Computer assisted instruction in natural resource managemeht. Twenty Sixth Annual Meeting. Southern Division.

American fisheries Society),

Knoxville, Tennessee. October 23-25,1972.

(With Franklin B. Titlow

12. Innovative teaching in fisheries science. One Hundred and Second Annual Meetino, A:nerican Fisheries Society, Hot Springs, Arkansas, Septeuber 10-13, 1972,

13. Restocking after fishkills as a fisheries management strategy. Tri-State Fisheries Conference, Rurr Oak State Park, Glouster, Ohio, February 14-16,1973.

(With William T. Bryson)

14. Use of catchability rates in brook, brown, and rainbow trout stocking programs. Tri-State Fisheries Conference, Burr Oak State Park, Glouster, Ohio, February 14-16, 1973. (With Dennis E. Hamond) l O

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-S-Robert T, Lack;y

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,O Poppiar Articles

'1. ' The landlocked alewife: landlocked salmon fond of the future?

1969. Maine fish and Game. 11(1):29.

2.

Shooting for better water. 1969. Colorado Outdoors. 18(6):40-42.

[ Reprinted: American Fishes and 11.5. TrVUt News. 1970. 15(1):12-14]

3.

The new fishery management. 1971. Colorido 0u'tdoors. 20(3):2-5.

4.

New life for dying lakes. 1971. Colorado Outdoors. 20(6):38-41.

5.

Teaching fisheries management: new role for computers, 1972.

Virginia Wildlife, 33(7):14-15. (With Franklin B. Titlow) 6.

Life beneath the surface. 1973. Colorado Outdoors. 22(1):36-38.

(WithJamesP.7pboy) 7.

Thermal pollution. 1973. Wonderful West Virginia. 36(12):10-13.

(With William T. Bryson) 8.

E.DeP. and fishery management. 1973. Virginia Wildlife. 34(2):16-18.

(With James R. Zuboy, Norville S. Prosser, and Raymond V. Corning) 9.

You can. judge a fish by its cover.

1973. Virttinia Wildlife.

34:(In Press).

(With William T. Bryson)

10. Channel catfish culture studies in Virginia. 1973. The Catfish farmer. S:(In Press).

(With Vaughn M. Douglass)

11. The fish hatchery. 1973. Wonderful West Virginia. 37(2):24-25.

(With Dennis E. Harmond) 12.

Fam pond management.

1973.

V.P.I. A Ltl. Extension Bulletin.

3 (Submitted).

(with Vaughn M. Douglass)

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