ML15273A440

Hdr Engineering. 2012. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2011

ML15273A440
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Site:	LaSalle
Issue date:	12/31/2012
From:	HDR Engineering
To:	Exelon Nuclear, Office of Nuclear Reactor Regulation
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ER REFERENCES TO BE DOCKETED ATTACHMENT 9

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li ZEBRA MUSSEL MONITORING PROGRAM At LaSalle Nuclear Station, 2011 Prepared for EXELON NUCLEAR Warrenville, Illinois HDR Engineering, Inc.

Environmental Science & Engineering Consultants I 0207 Lucas Road, Woodstock, Illinois 60098

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[! TABLE OF CONTENTS D Page No.

TABLE OF CONTENTS D LIST OF TABLES II LIST OF FIGURES Ill

1.0 INTRODUCTION

2 .0 METHODS 2 2.1 Artificial Substrate Sampling 2 2.2 Water Quality Measurements 4 3.0 RESULTS AND DISCUSSION 5

-- - - - - --3. I . --Artificial Substrate Sampling------ -- --* - -

• 3.1.1 Intake Substrates 5 3.1.2 Cooling Lake Substrates 5 3.2 Water Quality Measurements 5 3.2.1 Intake Water Quality 5 3.2.2 Cooling Lake Water Quality 6 4.0

SUMMARY

AND RECOMMENDATIONS 11

5.0 REFERENCES

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0 D

LIST OFTABLES D

Table No. Caption Page No.

D 3-1 Water Quality Parameters and Mean Zebra Mussel Densities D Collected Monthly During the 2011 Sampling Effort at LaSalle Intake. 7 3-2 Water Quality Parameters and Mean Zebra Mussel Densities Collected Monthly During the 2011 Sampling Effort at LaSalle Cooling Lake. 8

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LIST OF FIGURES D

Figure No. Caption Page No.

D 3-1 Temperature Profile at the LaSalle Station Intake for the D Period of 19 April to 9 November, 2011. 9 3-2 Temperature Profile at LaSalle Lake for the Period of 19 April to 9 November, 2011. 10 lll

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1.0 INTRODUCTION

D Exelon Nuclear has sponsored a zebra mussel (Dreissena. polymorpha) monitoring program since

-1990 (ZEI 2003, LMS 2004-2005, HDR/LMS 2006-2007, HDR Engineering, Inc. 2008-2011).

[] Stations electing to continue monitoring in 2011 include Braidwood Station, Byron Station, and

-LaSalle Station. Since the monitoring program began in 1990, zebra mussel colonization has occurred at all three of these stations, to varying degrees.

D The principal objectives of these monitoring efforts at LaSalle are to:

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• Detect the presence and densities of zebra mussels at the intake structures and source

~ water bodies.

• Evaluate year-to-year changes in the zebra mussel populations, including settlement

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[I Li 2.0 METHODS 0 2.1 ARTIFICIAL SUBSTRATE SAMPLING D' To detennine zebra mussel settlement densities, artificial substrates were placed in front of the LaSalle Station river intake in the Illinois River. Three arrays of artificial substrate samplers were

[l placed, which included two Type A substrates and one Type B substrate.

B Type A substrates consisted of two pieces of PVC pipe that were 15 cm (6 in.) long and had an inside diameter of 5.08 cm (2 in.) and an outside diameter of 6.08 cm (2.39 in.). Each pipe was cut in half fengthwise and rejoined using hose clamps. The PVC substrates were deployed by cable u from the railing above the intake in a central location. Concrete blocks were used to anchor each sampling array to the bottom of the intake. One cumulative substrate sampler and one monthly

~ (periodic) array was deployed at each sampling location. The PVC substrates were attached to the

-- -- - __G'AbJe_approximately _0..5 _and LS meters_below __the_surface _of_the_water, respectively. _Artificial i --substrates were installed in front of the intake on 7 April. Retrieval -of Type A-substrates occurred on a monthly basis and consisted of gently removing the series of PVC pipes from the water and D placing each individual sample into a labeled container containing a solution of 40% isopropyl alcohol. New PVC substrates were then placed on the monthly array and returned to their D original positions in the water column. Cumulative substrates remained in the water from 19 April to 9 November.

~ Type B substrate arrays consist of a microscope slide rack (Dura-Sampler) attached in the same

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manner as the PVC Type A arrays. Retrieval of Type B substrates occurred on a monthly basis and required gently removing the microscope slide samplers from the water and placing eight glass slides from each sampler into an appropriately labeled container containing 40% isopropyl D alcohol. New labeled glass slides were then installed into the slide samplers and returned to their original location. PVC and glass were chosen as principle substrates because it has been shown n that PVC is a preferred substrate (Walz 1975), while glass slides allow for a standard measurement of settlement densities and examination under a low powered binocular microscope

~ (Marsden 1992).

~ Two Type A and one Type B sample arrays were placed within the LaSalle Station Cooling Lake on 19 April. Substrates were set at depths of 1.0, 3.0 and 5.0 meters on a cable suspended

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[I Q between a surface float and a concrete anchor. Both cumulative and monthly substrates were set.

Monthly retrieval of Type A and Type B substrates was conducted as described above. Sampling 0 was concluded on 9 November with the removal of all the substrates from the LaSalle Station intake and cooling lake. Monitoring was designed to provide infonnation on accumulated infestation and D growth of settled zebra mussels throughout the growing season.

0 Settlement was determined by scraping the inside and outside of both halves of each PVC pipe sampler. The area of the substrate that was sampled was calculated by first finding the inside and outside circumferences of the PVC pipe.

D c=trd Where:

c = circumference of a circle

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1t=3.14 d = diameter of a circle 0 The total area of PVC pipe that was sampled was calculated by summing the total area sampled inside of the PVC pipe plus the total area sampled outside of the PVC pipe. The inside and outside D area of the PVC pipe was calculated using the following equation:

A=cxl Where:

A = area sampled c =circumference of a circle I= length of the PVC pipe The number of mussels found was then converted from number per square inch to the conventional number per square meter. Shell lengths (measured along the longest axis) were also measured for up to 50 individuals to obtain maximum, minimum, and mean sizes for each substrate. Shells less than 1.0 mm in length were measured to the nearest 25 microns using an ocular micrometer that was 3 HDR Engineering, Inc.

calibrated to a stage micrometer. Shells greater than 1.0 mm in length were measured to the nearest half-millimeter using a standard metric ruler.

2.3 WATER QUALITY MEASUREMENTS Four physicochemical parameters (temperature, dissolved oxygen, pH and conductivity) were measured in conjunction with the sampling program. These data were collected at each location prior to each sampling effort. All physicochemical measurements were made at one meter below the surface of the water. Temperature ( 0 C), dissolved oxygen (ppm), and conductivity (µmhos) were measured using an YSI Model 85 Oxygen, Conductivity, Temperature, and Salinity meter.

An Oakton WP pH Tester I was used to determine pH.

Temperature monitors (Onset OpticStowAway Temp Loggers) were attached at mid-depth to the cumulative and monthly substrates to record hourly changes in temperature throughout the entire

_ --- --=Sampling-period. -.I>eriodic-temperature-data -was --downloaded-on-a monthly-basis,--while

-cumulative temperature recordings *were monitored by a *separate logger from 19 April through 9 November.

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[ .Ii D 3.0 RESULTS AND DISCUSSION D 3.1 ARTIFICIAL SUBSTRATE SAMPLING D 3.1.1 Intake Substrates 0 No zebra mussels were collected on the monthly substrates from the LaSalle Station river intake throughout the seven month sampling period (Table 3-1 ). Analysis of the surface cumulative 0 substrate (O.Sm) revealed heavy filamentous algae covering the outside of the substrate. The inside of the pipe contained two zebra mussels (both 9 mm in length) numerous caddisfly larvae, n and byrozoan colonies. The two adult individuals attached to the pipe represent a settlement density of 37/m 2

• Two other adult zebra mussels were observed on the temperature probe and anchor brick. The bottom cumulative substrate (I .Sm) was covered both inside and outside with ITT caddisfly larvae and other aquatic invertebrates.

i -3 .1 .2. -Cooling-Lake Substrates No zebra mussels were collected on the monthly substrates from the LaSalle Station Cooling Lake throughout the seven month sampling period (Table 3-2). Cumulative substrates were 90-100% covered with bryozoan colonies. All depths sampled (1.0, 3.0, 5.0 m) were observed to have a similar degree of colonization.

3 .2 WATER QUALITY MEASUREMENTS Four physicochemical parameters (temperature, dissolved oxygen, pH and conductivity) were measured in conjunction with the sampling program. Water quality measurements were taken during each of the sampling dates at the LaSalle Station intake and cooling water lake (Table 3-1 and 3-2).

3.2. l Intake Water Quality Temperatures at the LaSalle Station intake ranged from 8.6° Con 21 April to 33.3° Con 21 July (Figure 3-1 ). Dissolved oxygen (DO) concentrations ranged from 4.5 ppm on 9 August to l 0.9 5 HDR Engineering, Inc.

ppm on 19 April (Table 3-1). Recordings for pH ranged from 7.4 to 8.0 and conductivity ranged from 551to874 µmhos throughout the sampling season.

3.2.2 Cooling Lake Water Quality Temperatures from the LaSalle Station cooling lake ranged from 15.5° Con 21 April to 37.7° C on 2 August (Figure 3-2). Dissolved oxygen (DO) concentrations ranged from 4. 7 ppm on 19 July to 10.1 ppm on 4 May (Table 3-2). Recordings for pH ranged from 8.1 to 8. 7 and conductivity ranged from 1270 to 1392 µmhos throughout the sampling season.

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~ ~ ~ ~ ~ ~ ~ ~ ~ ~ lmJ!! ~ ~ r~*~J c=1 f**) I* J ~*'*1 r-. *1 Table 3-1. Water Quality Parameters and Mean Zebra Mussel Dejsities Collected Monthly During the 2011 sampling Effort at LaSalle Intake. I I

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I Water Quality I Juveniles Adults I

Days/ Temp. DO Cond. , Sample Date Sample Time coq (ppm) pH (µmhos) '. Depth Glass PVC Glass PVC 04/19/11 08:10 10.8 10.9 7.8 874 05/04/11 15 09:00 13.0 10.4 7.7 565 0 0 0 0 06/01/11 28 08: 11 19.7 7.7 7.9 551 I 0 0 0 0

-...J 07/19/11 48 08:15 30.2 6.4 7.7 830 0 0 0 0 I

08/09/11 21 08:11 29.5 4.5 7.4 724 0 0 0 0 09101/11 23 12:08 28.7 9.4 7.8 765 I 0 0 0 0 I

10/05/11 34 09:05 17.7 7.4 7.9 696 0 0 0 0 11/09/11 35 09:15 13.6 10.6 8.0 720 I 0 0 0 0 Cumulative 0.5m 2

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Water Quality i Juveniles Adults II Days/ Temp. DO Cond. ISample I Date Sample Time (OC) (ppm) pH (µmhos) Depth Glass PVC Glass PVC 04119111 09:09 15.3 9.8 8.1 1270

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00 06101111 28 09:06 25.8 8.4 8.5 1315 i 0 0 0 0 I

07/19/11 48 08:54 33.6 4.7 8.3 1392 I 0 0 0 0 I

08/09/11 21 08:50 34.3 3.1 8.3 1300 0 0 0 0 i

I 09/01/11 23 11 :36 32.4 8.1 8.1 1375 0 0 0 0

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I 10/05/11 34 09:50 25.4 9.1 8.5 1392 I 0 0 0 0 l

I 11109/11 35 10:05 19.3 9.5 8.5 1296 I 0 0 0 0

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N Figure 3-1. Temperature Profile at the LaSalle Station IntakJ for the Period of 19 April to 9 November, 2011.

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4.0

SUMMARY

AND RECOMMENDATIONS In 2011, no zebra mussels were found on the periodic (monthly) substrates from the river intake or LaSalle Lake locations. Cumulative substrate samples retrieved from the river intake contained two adult zebra mussels which represent a settlement density of 3 7/m 2* Two additional adult specimens were observed on the temperature probe and anchor block. The cumulative 0 substrates in LaSalle Lake were 90-100% covered by bryozoan colonization at all sample depths.

Early detection of zebra mussel colonization is essential for uninterrupted Station operation.

Because zebra mussels are present in the Illinois River, they are continually reintroduced to the cooling lake through make-up water. Continued monitoring is important to recognize the circumstances associated with zebra mussel infestation and colonization.

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5.0 REFERENCES

0 HDR Engineering, Inc. 2011. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2010. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.

HDR Engineering, Inc. 2010. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2009. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.

HDR Engineering, Inc. 2009. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2008. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.

HDR Engineering, Inc. 2008. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2007. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.

HDR/LMS. 2007. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2006.

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Prepareo forExelon Nuclear-:-Warrenville,lL-:-12 pp.--

HDR/LMS. 2006. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2005.

Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.

Lawler, Matusky & Skelly Engineers, LLP (LMS). 2005. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2004. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.

Lawler, Matusky & Skelly Engineers, LLP (LMS). 2004. Zebra Mussel Monitoring Program at LaSalle Nuclear Station, 2003. Prepared for Exelon Nuclear. Warrenville, IL. 12 pp.

Marsden, J. E. I 992. Standard Protocols for Monitoring and Sampling Zebra Mussel Illinois Natural History Survey. Biological Notes 138, April 1992.

Walz, V. N. 1975. The Settlement of Larvae of Dreissena polymorpha on Artificial Substrates. Arch. Hydrobiol. Suppl. 47: 423-431.

Zambrana Engineering, Inc. 2003. Byron Station 2002 Zebra Mussel (Dreissena polymorpha)

Monitoring Program Annual Report.

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