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NPDES Permit No. SC0030856 Renewal Application, Appendix B, Entrainment Study, 2016 and Revised 2017
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Issue date:	02/28/2017
From:	; Normandeau Associates, South Carolina Electric & Gas Co
To:	; Office of Nuclear Reactor Regulation
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APPENDIXB VC Summer Nuclear Station Entrainment Study, 2016 and revised 2017 (Contains complete report by Normandeau, 2009b)

V.C. Summer Nuclear Station Entrainment Study - 2016 and revised 2017.

Prepared for: SCANA RFP WK9622-(2015)

Submitted By Normandeau Associates, Inc.

25 Nashua Road Bedford, NH 03110-5500 603.472.5191 www. normandeau. com February 28, 2017

V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Table of Contents Page

1.0 INTRODUCTION

.................................................................................................................... 1 2.0 FACILITY DESCRIPTION............................................................................. :..................... 1 3.0 METHODS AND MATERIALS............................................................................................ 2 4.0 RESULTS................................................................................................................................. 5 5.0 DISCUSSION........................................................................................................................... 9 6.0 LITERATURE CITED............................................................... :......................................... 12

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APPENDICES APPENDIX 1. Geosyntec Consultants. 2015. Entrainment sampling plan Virgil C. Summer Nuclear Station Unit 1. Prepared for South Carolina Electric and Gas Company. Kennesaw, GA.

APPENDIX 2. Normandeau Associates, Inc. 2009. Monticello Reservoir lchthyoplankton Studies, September 2008 through August 2009. Prepared for South Carolina Electric and Gas Company. Bedford, NH.

APPENDIX 3. Normandeau Associates, Inc. 2016: Quality assurance plan and standard operating procedures for entrainment sampling at Virgil C. Summer Nuclear Station, Jenkinsville, South Carolina. Prepared for South Carolina Electric and Gas Company. Stanley, NC.

APPENDIX 4. Field sampling audit report of Mr. Bob Hasevlat resulting from his June 22, 2016, observations at Monticello Reservoir.

APPENDIX 5. Field sampling audit report of Mr. Fred Heitman resulting from his July 27, 2016, observations at Monticello Reservoir.

APPENDIX 6. Detailed Comments on report ofV. C. Summer entrainment study, 2016 by Charles C. Coutant.

Summer Station 2016 lchthyo Final Report-2017 Additions.docx 2/28/17 11 Normandeau Associates, Inc.

V. C. SUMMER NUCLEAR STATION ENTRAINMENT StUDY.:.. 2016 AND REVISED 2017 List of Figures Page Figure 2-1.

Location of the Virgil C. Summer Nuclear Station along the shoreline of Monticello Reservoir in South Carolina............................................................................. 15 Figure 2-2.

Site layout for Virgil C. Summer Nuclear Station on Monticello Reservoir, the Fairfield Pumped Storage Facility, and Parr Reservoir (from Geosyntec 2015).

Ichthyoplankton entrainment collections in 2016 were made from the vicinity of the Unit 1 Intake while mention of previous ichthyoplankton collections in 2008

-2009 (Normandeau 2009) was from the vicinity of the Units 2 & 3 Intake................... 16 Figure 2-3.

Plan view of the Summer Station Unit 1 CWIS showing the path of the CWIS flow between the concrete retaining walls (brown) into one of six intake bays and the location of six traveling screens (blue) and three circulating water pumps (SCANA, unpublished data)............................................................................................... 16 Figure 2-4.

Cross-section view of the Summer Station Unit 1 CWIS showing the path of the CWIS flow past the trash rack (red) extending from the bottom of the skimmer wall (brown), 3/8" square mesh traveling scree11 (blue), and bar grid prior to encountering the circulating water pump (SCANA, unpublished data)............................ 17 Figure 2-5.

Maximum area of hydraulic influence (cross-hatched area) derived by ADCP measurement and ichthyoplankton entrainment sampling transects (vertical lines) used in 2016 near the Summer Station Unit 1 Intake (from Geosyntec 2015).

Horizontal lines represent transects sampled in 2008 - 2009 (Normandeau 2009) for the future Summer Station Units 2 & 3 Intake structure.............................................. 1 7 Figure 3-1.

Paired bongo nets and flowmeters used to sample ichthyoplankton in Monticello Reservoir (bottom photo courtesy of Fred Heitman)......................................................... 18 Figure 3-2.

Paired plankton net sampling cups prior to rinsing (top) and the preservation of a composited sample from previous Monticello Reservoir ichthyoplankton studies (top photo courtesy of Fred Heitman)................................................................................ 19 Figure 3-3.

Collection of water quality data during Monticello Reservoir ichthyoplankton studies.................................................................................................................................. 20 Figure 3-4.

Analyst sorting ichthyoplankton samples collected during Monticello Reservoir ichthyoplankton studies....................................................................................................... 20 Figure 4-1.

Monthly counts of ichthyoplankton collected within the area of hydraulic influence in Monticello Reservoir near the Summer Station CWIS, March through August 2016............................................................................,.............................. 21 Figure 4-2.

Familial composition of ichthyoplankton collected within the area of hydraulic influence in Monticello Reservoir near the Summer Station CWIS, March through August 2016...................................................................................................... :.... 21 Figure 4-3.

Daytime water temperatures measured at three depths during ichthyoplankton sampling in Monticello Reservoir, March through August 2016...................................... 22 Figure 4-4.

Nighttime water temperatures measured at three depths during ichthyoplankton sampling in Monticello Reservoir, March through August 2016...................................... 22 Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 111 Normandeau Associates, Inc.

V. C. SUMMER NUCLEAR STATION ENTRAiNMENT STUDY-2016 AND REVISED 2017 Figure 4-5.

Daytime dissolved oxygen concentrations measured at three depths during ichthyoplankton sampling in Monticello Reservoir, March through August 2016........... 23 Figure 4-6.

Nighttime dissolved oxygen concentrations measured at three depths during ichthyoplankton sampling in Monticello Reservoir, March through August 2016........... 23 Figure 4-7.

Depth-averaged ichthyoplankton density (no./100 m3) for the day and night period. The vertical dashed lines delineate the sample populations for bootstrapping entrainment abundance during high-abundance season between 15 May and 15 July and the combined low-abundance periods at the tails of the time series...............................................,.................................................................................... 24 Figure 4-8.

Percent familial composition of estimated A) day, B) night, and C) total ichthyoplankton withdrawn by the V.C. Summer Nuclear Station considering the ichthyoplankton density in Monticello Reservoir near the intake and the amount of water withdrawn by the station, March through August 2016...................................... 25 Figure 4-9.

Estimates of the median and upper and lower 95% confidence limits for the numbers of ichthyoplankton of all taxa and life stages entrained by the V. C.

Summer Nuclear Station annually based on the displayed probability distribution of a bootstrap analysis of 10,000 runs. ****************************************:************************************** 26 Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 IV Normandeau Associates, Inc.

V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-:- 2016 AND REVISED 2017 Table 3-1.

Table 4-1.

Table 4-2.

Table 4-3.

Table 4-4.

Table 4-5.

Table 4-6.

Table 4-7.

Table 4-8.

List of Tables Allocation of calculated V.C. Summer water withdrawal volume (million cubic meters) among sample period based on mean daily proportion of each diel period (based on the calendar).and per sample period for depth-averaged ichthyoplankton density estimates. The plant operated as a base-load generating Page facility and exhibited negligible variation in flows during this study............................... 27 Month, common name, and counts of ichthyoplankton collected in Monticello Reservoir, March through August 2016. YSL = yolk-sac larvae, PYSL = post yolk-sac larvae, ULS = undetermined larval stage, and YOY = young-of-the-year...................................................................................................................................... 28 Date, time, depth (m), temperature (

0C), and dissolved oxygen concentration (DO, mg/1) associated with ichthyoplankton sampling in Monticello Reservoir, March through August 2016............................................................................................... 29 Environmental conditions associated with ichthyoplankton sampling in Monticello Reservoir March through August 2016........................................................... 31 Daily water usage for in-plant needs (million gallons/day) and monthly totals (million gallons/month) through the Summer Station CWIS from March through August 2016. Shaded cells represent ichthyoplankton sampling dates, horizontal lines indicate the cutoff between first-half and second-half of the month for entrainment calculations..................................................................................................... 32 Daily water usage for circulation water needs (million gallons/minute) and monthly totals (million gallons/month) through the Summer Station CWIS from March through August 2016. Shaded cells represent ichthyoplankton sampling dates, horizontal lines indicate the cutoff between first-half and second-half of the month for entrainment calculations.............................................................................. 33 Mean ichthyoplankton density (no.II 00 m3), listed in decreasing order of overall density, for each fish taxon, life stage and sampling depth at VC Summer Nuclear Station, averaged over diel and sample periods from 1 March through 31 August 2016. YSL "". yolk-sac larvae, PYSL = post yolk-sac larvae, ULS =

undetermined larval stage, and YOY = young-of-the-year................................................ 34 Mean depth-averaged ichthyoplankton density (no.II 00 m3) by fish taxon, life stage, diel period and month at VC Summer Nuclear Station representative of the period from 1 March through 31 August 2016. YSL = yolk-sac larvae, PYSL =

post yolk-sac larvae, ULS = undetennined larval stage, and YOY = young-of-the-year. **************************.***********************:************************************************.. *************************** 35 Estimated numbers of ichthyoplankton entrained at VC Summer Nuclear Station (millions) by fish taxon, life.stage, and diel period summed for all dates from from 1 March through 31 August 2016, considering ichthyoplankton densities near the station and amount of water withdrawn. YSL = yolk-sac larvae, PYSL

= post yolk-sac larvae, ULS = undetermined larval stage, and YOY = young-of-the-year............... *................................................................................................................. 36 Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 V

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V. C. SUMMER NUCLl=.AR STATION ENTRAINMENT STUDY - 2016 AND REVISED 2017 Table 4-9.

Estimated numbers of ichthyoplankton entrained at VC Summer Nuclear Station (millions) by fish tax.on, life stage, and diel period for each month from 1 March through 31 August 2016, considering ichthyoplankton densities near the station and amount of water withdrawn. YSL = yolk-sac larvae, PYSL = post yolk-sac larvae, ULS = undetermined larval stage, and YOY = young-of-the-year....................... 37 Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 VI Normandeau Associates, Inc.

V. C. SUMMER. NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017

1.0 INTRODUCTION

Ichthyoplankton (fish eggs and larvae) samples were collected in Monticello Reservoir to provide estimates of ichthyoplankton entrainment for the South Carolina Electric & Gas (SCE&G) V.C. Summer Nuclear Station Unit 1 Cooling Water Intake Structure (NPDES SC0030856). V.C. Summer Nuclear Station (Summer Station) is a base-load generating facility that withdraws water from Monticello Reservoir for internal plant systems and circulating water to cool the Unit 1 reactor; maximum annual flows during a non-outage year could slightly exceed 270 billion gallons (1 billion cubic meters (m3)).

Roughly half of that maximum flow could carry fish eggs and larvae during the typical six-month South Carolina freshwater fish spawning period that lasts from March through August. A South Carolina Department of Health and Environmental Control (SCDHEC) directed study plan was developed by Geosyntec Consultants (Geosyntec) to formalize the collection of ichthyoplankton data per EPA Clean Water Act Section 316(b) requirements ( Geosyntec 2015, Appendix 1). The purpose of this study was to execute that study plan and collect ichthyoplankton density data from Monticello Reservoir in the area of hydraulic influence of the Summer Station Cooling Water Intake Structure (CWIS) and then to estimate the number of ichthyoplankton potentially entrained by actual CWIS withdrawals during the fish spawning season of 2016. The entrainment data collected in this plan, in addition to ichthyoplankton data collected in 2008 -2009 at the nearby future site of the Summer Station Units 2 and 3 Intake Structure (Normandeau 2009, Appendix 2), will assist the SCDHEC in determining whether Summer Station Unit 1 will be required to submit further information as outlined in 40 CFR 122.21(r)(7) through (r)(13).

2.0 FACILITY DESCRIPTION (Per Geosyntec 2015)

Summer Station Unit 1 is a.972.7-megawatt, nuclear-fueled, base-load generating facility located along the shoreline of Monticello Reservoir near Jenkinsville, Fairfield County, in north-central South Carolina (Figure 2-1 ). Summer Station uses a once-through cooling water system with a design intake capacity of approximately 533,122 gallons/minute or 768 million gallons/day (MGD). It withdraws cooling water from Monticello Reservoir via a single shoreline CWIS located at the south end of the reservoir (Figure 2-2). A jetty protrudes from the southern shoreline of the reservoir and serves to direct thermal discharge flows uplake and away from intake flows withdrawn at the Summer Station CWIS. Monticello Reservoir was constructed for the purpose of serving as part of the cooling water system (Nuclear Regulatory Commission 2004). The use of Monticello Reservoir as a cooling impoundment for Summer Station Unit 1 has been determined by SCDHEC to be a "closed-cycle recirculating system" under 40 CFR, Part 125, Subpart J, § 125.92( c )(2). Monticello Reservoir is also the upper reservoir for the Fairfield Pumped Storage Station (FPSS), with Parr Reservoir and the Broad River (Santee Basin) serving as the lower pool. Generation flows at the FPSS (typically during daytime hours) can lead to minor reservoir water level declines while nighttime pumpback operations act to increase reservoir water levels and create turbulent mixing near the vicinity of Monticello Reservoir where Summer Station draws CWIS flows.

Mixing from FPSS pump back may act to provide a more even distribution of ichthyoplankton within the water column.

The Unit 1 CWIS at Summer Station consists of an inlet bay about 550 ft wide east to west and about 200 ft in length north to south. The water depth in the bay ranges from 30 to 40 ft. The CWIS is 93 ft wide with six intake bays each approximately 13-ft wide (Figure 2-3). Parallel concrete retainer walls extend out into the intake bay of the reservoir a distance of approximately 30 ft. Trash racks composed of steel bars with IO-inch (in) spacing are located along the upstream face of the intake structure to prevent large Summer Station 2016 lchthyo Final Report-2017 Additions.docx 2128/17 1

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V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 debris from entering the intake bays (Figure 2-4). The trash racks are mounted to the bottom of a skimmer wall that extends from the water surface to a depth of 9.5 ft (415.5 ft mean sea level [MSL NGVD29]) at normal high water (425 ft MSL). The skimmer wall is designed to exclude floating debris from entering the cooling water system and, combined with the intake retainer walls, to optimize withdrawal of the coolest water from the water column at the pump house. Vertical traveling water screens (3/8 inch square mesh opening) are located 25 ft behind the trash racks to strain out smaller debris. A bar grid structure is located between the traveling screens and the circulating pumps. Three circulating water pumps convey screened flow to the condensers. At normal high water, the CWIS is designed to withdraw water from the water column between the 415.5 and 390 ftMSL; or from a depth of9.5 to 35 ft (2.9 to 10.7 m). As Monticello Reservoir averages 59 ft deep (maximum depth of 125 ft), it is unlikely that the surface-oriented CWIS is capable of withdrawing the coldest (densest) water in the reservoir.

The area of hydraulic influence was defined as the area of Monticello Reservoir near the Summer Station Unit 1 CWIS where ichthyoplankton would encounter any velocity vector oriented towards the CWIS.

Acoustic Doppler Current Profiler (ADCP) surveys occurred at five transects of increasing distance from the normally operating CWIS under three reservoir elevation scenarios (Geosyntec 2005). Those scenarios included high and low reservoir elevations and a third where the reservoir elevation was high but declining due to generation flows at the FPSS. A maximum area of hydraulic influence measuring 2.92 surface acres, extending out into the reservoir approximately 550 ft from the CWIS with a width of about 250 ft, was delineated (Figure 2-5).

3.0 METHODS AND MATERIALS Sampling Site and Larval Fish Collections - Ichthyoplankton sampling was conducted in Monticello Reservoir within the area of hydraulic influence of the Unit 1 CWIS. Day and Night samples were collected at both the surface (within the top 1 m of the water column) and mid-depth ( estimated depth of 5 m). Sampling occurred twice per month between 1 March and 31 August, 2016, and resulted in the collection of 48 samples (6 months x 2 samples/month x 2 diel periods/sample x 2 depth strata/period) per the Geosyntec Study Plan (Appendix 1). Sampling events were* separated by a minimum of seven days.

Day sampling occurred at least two hours after sunrise and two hours before sunset and night sampling occurred at least two hours after sunset and two hours before sunrise. Tows were conducted

perpendicular to the shoreline using paired 0.5 m diameter plankton bongo nets equipped with 0.300 mm mesh and calibrated flowmeters (Figure 3-1). The target volume for each side of the bongo was 50 m3 and the two sides were composited for an approximate total volume of 100 m3 per sample.

Larval fish entrainment tows and field chemistry data were collected by Normandeau's Aiken, SC, lab (SCDHEC Certified Laboratory ID #02101). Staff from this laboratory conducted the 2008 through 2009 larval fish collections on Monticello Reservoir for future Summer Station Units 2 and 3 (Normandeau 2009). Procedures followed in 2016 were outlined in the Quality Assurance Plan and Standard Operating Procedures for Entrainment Sampling at Virgil C. Summer Nuclear Station, Jenkinsville, South Carolina (Normandeau 2016, Appendix 3). Ichthyoplankton samples were preserved in 5% formalin and Chain of Custody records initiated (Figure 3-2). Preserved samples were securely packaged and transported to the

Aiken, SC, lab at the conclusion of the sampling event.

Water Quality and Environmental Data Collection -For each day or night sampling event, water temperature (to 0.1 °C) and dissolved oxygen (DO, nearest 0.1 mg/1) were measured with a YSI 6920 multiparameter sonde (Figure 3-3). Measurements were made near surface (0.3 m.), at mid-depth (6.1 -

Summer Station 2016 lchthyo Final Report. 2017 Additions.docx 2/28/17 2

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. V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED. 2017 7.6 m.), and near bottom (12.2 -15.2 ru.). Water quality sampling occurred within the area of the ichthyoplankton sampling event and at a location of sufficient depth to acconnnodate all three sampling depths. Air temperature (

0 C), observations on reservoir conditions, and weather conditions were also recorded.

Plant operational data were obtained from SCE&G staff to provide estimates of CWIS flows during the months of March through August 2016. Each month was divided into approximate halves so the first and second entrainment samples could be rel~ted to more accurate semi-monthly CWIS flows. Semi-monthly analysis of larval fish densities and calculated entrainment estimates doubled the number of data points for more refined* entrainment estimate confidence interval calculations. As the first larval fish collection did not occur until March 18, CWIS flows for the first half of March extended from March 1 to 18 while second-half flows included the remainder of the month (Table 3-1).. All other monthly flows extended from the 1st to the 15th of the month with second-half flows encompassing the remaining days.

Sunrise and sunset times for Columbia, SC, were obtained from the United States Naval Observatory at http://aa.usno.navy.mil/data/docs/RS OneYear.php for use in determining the length (or daily proportion) of day and night periods (Table 3-1 ). Diel analysis of entrainment data again doubled the number of data points for more refined entrainment confidence interval calculations.

Field Sampling Quality Assurance-To assure compliance with the field sampling portions of the standard operating procedure (Normandeau 2016), two audits of the field sampling crews were conducted at Monticello Reservoir. One audit was conducted by Robert Hasevlat, Normandeau Associates, Inc.,

Director of Quality Assurance/Health and Safety while the second was conducted by Senior Aquatic Ecologist and American Fisheries Society Certified Fisheries Professional Fred Heitman ofEnercon Services, Inc.

Sample Processing and Taxonomy - Ichthyoplankton samples and accompanying Chain of Custody records were shipped to the Normandeau Larval Fish Taxonomy Lab in Bedford, NH (SCDHEC Certified Lab ID# 80003) for the identification of ichthyoplankton. Upon arrival in Bedford, samples were inspected to ensure that their condition and the accompanying Chain of Custody records were acceptable.

Samples were logged into the laboratory tracking system and stored under the appropriate conditions.

Under ventilation, the sample preservative was drained and the sample gently washed through a clean 0.300 mm mesh sieve in preparation for sorting. If large numbers of eggs and larvae were present, the sample was subsampled using a Folsom Splitter, to a quota of 100 eggs and 100 larvae (all species and life stages combined). Complete sorting of any split in which a quota was met occurred. If the target quota for either eggs or larvae was not reached, subsequent splits were sorted until the target was reached for either eggs or larvae.

Normandeau taxonomists for identification of the Sunnner Station ichthyoplankton included Joe Strube and Alan Bullock. Collectively, they have identified freshwater ichthyoplankton for over 70 studies from across the United States. Strube' s ichthyoplankton and juvenile fish taxonomy expertise is the result of a professional career of over 37 years in fisheries related work. Alan Bullock has spent 7 years exclusively focused on the identification of freshwater and estuarine ichthyoplankton, with a minor focus on marine species. Ichthyoplankton identifications were based on larval fish keys and distribution information of adult fish (Auer 1982, Bulak 1985, Fuiman 1978, Jenkins and Burkhead 1994, Kuehne and Barbour 1983, Lee et al. 1980, Lippson and Moran 1974, McGowan 1984, McGowan 1988, Menhinick 1991, Rohde et al. 2009, Scotton et al. 1973, Simon and Wallus 2006, and Wallus and Simon 2008) to the lowest Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 3

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V. c: SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 practical taxon, enumerated, and assigned a life stage: egg, yolk-sac larvae, post yolk-sac larvae, young-of-the-year, or juvenile (Figure 3-4). In a few instances, larvae were id~ntified as having an undetermined larval stage due to ambiguous size and the lack of distinguishing guts and/or yolk sac. For each sample, a maximum of 25 representative individuals from each fish taxon were measured for total length. A voucher collection was prepared.

Larval Density-Ichthyoplankton densities were calculated with flowmeter data (providing actual volumes sampled) and the numbers counted in samples, which were then standardized to number/100 m3*

Differences in ichthyoplankton sample densities between depths or diel periods were examined by paired t-tests oflog10(x+ 1) transformed den*sities. Tests of the t-statistic confirmed the strategy of averaging the Surface and Mid-depth data and ichthyoplankton sample densities were averaged across depth for each sampling date and diel period, resulting in 12 daytime and nighttime estimates of ichthyoplankton density. Depth-averaged daytime and nighttime densities were assumed representative of the true densities within each respective sample period ranging from 13 to 18 days in duration from 1 March through 31 August 2016.

Entrainment Estimates - The numbers of ichthyoplankton entrained under plant operations were estimated by ichthyoplankton sample densities and the volumes of water withdrawals. In recognition of potential diel differences in day and night ichthyoplankton density and that day and night periods become unequal in duration during the sumrrier season, daily entrainment abundance within each sample period was estimated by multiplying the diel-specific density estimate by the corresponding daily water withdrawal volumes and the daily proportion of time for the diel period. The daily proportion of time for each diel period was based on the duration (hours) between sunrise and sunset for the daytime period (Pctay) and the complement for the nighttime period (1-Pctay) based on sunrise/sunset times for Columbia, SC.

Annual entrainment abundance at the Summer Station CWIS from 1 March through 31 August 2016 was estimated by summation of daily diel entrainment estimates across all fish taxa and life stages. The calculation was performed as follows:

E

= ~Nperiods~Ndays~Nfishtaxa~Nstages[(Dp,t,f,s,dayv. p

) + (Dp,t,f,s,nightv p

)] (E

1) annual

.l.,p=1 Llt=1 L,[=1 Lls=1 100 p,t p,t,day 100 p,t p,t,mght

q.

where Eannual =

Nperiods =

Nr,sh raxa =

Nstages =

D p,rj.s,day.-

Dp,1J;;,nighr =

annual entrainment abundance of all fish taxa and life stages combined, number of sample periods, number of days in sample period p, number of fish taxa, number of life stages, depth-averaged ichthyoplankton sample density (number per 100 m3) of fish taxonf and life stages representative of the day period for day tin sample period p,

depth-averaged ichthyoplankton sample density (number per 100 m3) of fish taxonf and life stages representative of the night period for day tin sample

periodp, water volume (m3) withdrawn by the CWIS on day tin sample period p, Summer Station 2016 lchthyo Final Report-2017 Additions.docx 2/28/17 4

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. V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Pp,1,day =

Pp.r.night =

proportion of day tin sample period p that is the day period define by the duration between sunrise and sunset, and proportion of day tin sample period p that is the night period (=1-Pp,r,da)-

Uncertainty in the annual entrainment estimate was estimated by non-parametric bootstrapping, which is a special case of the Monte Carlo simulation method. Monte Carlo simulations use a mathematical model of the process being estimated, where some of the model parameter values are randomly selected from a theoretical probability density distribution (e.g., normal, lognormat beta, gamma; etc.). Herein, the bootstrapping method randomly selects from a sample population (i.e., empirical distribution), with replacement, as if it was a continuous probability distribution (Haddon 2001). The bootstrapping method assumes the observed ichthyoplankton sample densities are representative of the true ichthyoplankton sample density and the underlying probability density distribution of expected ichthyoplankton sample density values.

In this study, where entrainment was sampled by a stratified design.without replication, depth-averaged ichthyoplankton sample density estimates for day and night periods were considered a representative sample population within a season of similar magnitude in density values. This approach minimizes the potential introduced bias from a sample population that has a seasonal trend (i.e., homogeneous and independent). Each iteration of a bootstrap simulation using Equation 1, a daytime and nighttime ichthyoplankton sample density was randomly selected for a given day from the subset of corresponding diel-specific estimates during a season oflow abundance (e.g., the tails of the study period) or high abundance (e.g., the peak season). The 95% confidence limits of the annual entrainment abundance estimate were determined by the corresponding percentiles of the distribution of bootstrapped entrainment abundance replicates from 10,000 iterations of Equation 1. Because the median of the distribution of bootstrap estimates often differ from the observed parameter estimate, the percentiles corresponding to the lower and upper 95% confidence limits were*adjusted from the 2.5 1h and 97.51h percentile to account for this bias (Haddon 2001 ).

The bias-corrected 95% confidence limit of the annual entrainment abundance estimate was detennined from the lower and upper percentiles (P1awer, o,95 and Pupper, o.95) of the distribution of I 0,000 bootstrap estimates as follows:

Plower o 95 = <P(2zo - 1.96)

Pupper,0.95 = <P(2zo + 1.96)

(Eq. 2)

(Eq. 3)

(Eq. 4) where <l>-1 is the inverse cumulative normal distribution function, ct> is the cumulative normal distribution function, and Fis the fraction of bootstrap estimates below the annual entrainment abundance estimate from Equation 1.

4.0 RESULTS Sampling Site and Larval Fish Collections - Normandeau staff collected ichthyoplankton within the area of hydraulic influence on 12 dates throughout the spring and summer of 2016. They were accompanied by SCE&G staff on two occasions and by Bob Hasevlat, Normandeau Associates, Inc., and Fred Heitman, Enei.-con Services, on one occasion each for QA audits. Ichthyoplankton occurred in samples from March through August *2016, though numbers collected in those two months were the lowest Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 5

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V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 observed (Figure 4-1, Table 4-1). A total of 1,311 organisms comprising seven fish families were collected with over half (50.8%) occurring during the month of June. Larval fish (yolk-sac larvae, post yolk-sac larvae, or undetermined larval stage) dominated collections with only one egg (Dorosoma species) and five young-of-the-year catfish (Blue and Channel Catfish) comprising other life stages. No federal or state protected species were identified in ichthyoplankton samples and none would be expected based on fish distribution data or the SCDNR Heritage Trust Program list for Fairfield County, SC.

The ichthyoplankton was dominated by members of the Clupeid family (Dorosoma genus) who comprised over 86% of all organisms collected (Figure 4-2). Centrarchidae comprised 9.6%, Cyprinidae 1.6% and the Catostomidae, Ictaluridae, Moronidae, and Percidae each comprised <1 % of the total number collected.

Water Quality and Environmental Data Collection - Surface, Mid-depth, and Deep water temperatures were measured during Day and Night tows with each sampling event (Figures 4-3 and 4-4, and Table 4-2). Daytime water temperatures ranged from a low of 12.8 °C in a Deep sample in March to a high of 31.3 °C in a Surface sample in late-July and were generally similar to those measured at night (range of 12.2 °Cina Deep sample in March to a high of30.9 °Cina Surface sample in late-July). Visual comparison of the figures suggested that Day and Night temperatures differed imperceptibly at each depth strata. Deep water temperatures were cooler than those measured at the surface and Mid-depth temperatures were always intermediate between those collected in Deep and Surface samples. Deep and Mid-depth temperatures become more similar as the summer progressed and the water column became isothermal in mid-August. Based on regional weather patterns, reservoir cooling typically begins in late-August or September.

Surface, Mid-depth, and Deep-DO concentrations were measured during Day and Night tows with each sampling event (Figures 4-5 and 4-6, and Table 4-2). Daytime DO concentrations ranged from a high of 11.5 mg/I in a Surface sample in April to a low of 2.9 mg/I in a Deep sample in mid-July. Night DO concentrations exhibited more variability compared to Day concentrations, especially for Mid-depth and Deep samples. Pump back flows from FPSS may account for some of this variability. Nighttime DO concentrations ranged from a high of 11.2 mg/1 in a Surface sample in April to a low of 3.0 mg/I in a Deep sample in late-July. Mid-depth DO concentrations were lower than Surface DO concentrations and generally higher than those at Deep depths.

Weather conditions during the March through August sampling events were generally good, discounting excessive air temperatures that exceeded 30 °Con six of the 12 sampling dates (Table 4-3). Light precipitation was the heaviest rainfall observed and it was confined to one nighttime sampling event.

Winds producing a light chop on the water surface occurred on only four of the 12 sampling dates; otherwise the water surface was calm. No environmental anomalies were recorded that would have impacted the collection oflarval fish samples.

Flow data were provided by SCE&G for the 2016 sampling period. Flows at the Summer Station CWIS consist of in-plant uses and once-through cooling water. In-plant usage flows are relatively low and vary daily (Table 4-4) while once-through cooling water needs during non-outage periods are high and consistent at 512,998 gallons/min (Table 4-5). During the March through August 2016 fish spawning period, monthly CWIS flows ranged from 22,175.1 to 22,916.0 million gallons (83,941,738.9 to 86,746,420.9 m3). CWIS flows each month were separated into flows associated with the first entrainment sample (i.e., flows during the first half of the month) and flows associated with the second entrainment sample (i.e., flows during the second half of the month) as described previously (Table 3-1).

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V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 A.ND REVISED 2017 Field Sampling Quality Assurance - Mr. Bob Hasevlat, Normandeau Associates, Inc. and Mr. Fred Heitman, Enercon Services, Inc. provided field audits of the sampling on June 22, 2016 (Appendix 4),

and July 27, 2016 (Appendix 5), respectively. Mr. Hasevlat provided additional observations of laboratory documentation procedures on October 21, 2016. Both auditors indicate that field sampling was conducted per the QA Plan (Normandeau 2016) and that non-conformities in data collection were not observed. Additionally, Mr. Hasevlat found that chain of custodies forms, sample logs, data sheets, and quality records at the Bedford Larval Fish Taxonomy Lab were complete.

Sample Processing and Taxonomy - Ichthyoplankton samples were logged, checked, prepared, sorted, and identified by the standard laboratory operating procedures of the Normandeau Larval Fish Taxonomy Lab in Bedford, NH. Seven families of fish and nine individual species were observed (Figure 4-2 and Table 4-1); all have been previously observed or documented within the Broad River Basin of the Santee Drainage (Normandeau 2009 and Rohde et al. 2009). The reference vials for the Summer Station ichthyoplankton study are located in a lockable reference vial collection vault at the Normandeau Larval Fish Taxonomy Lab.

Larval Density - There was no significant difference in the log10(x+ 1 )-transformed total ichthyoplankton density between the surface and mid-depth during the day period (Paired t-test, t=-1.03, P = 0.327) and night period (Paired t -test, t=-1.03, P = 0.327). Larval densities varied non-significantly by depth but were generally higher in Surface than Mid-depth waters (Table 4-6).

Mean ichthyoplankton density was typically higher during the night than during day throughout the study period (Figure 4-7, Table 4-7). The mean difference between log10(x+ 1 )-transformed nighttime and daytime density was significantly higher than zero (Paired t-test, t =4.00, P = 0.002) indicating significantly higher densities at night. The dominant taxon and life stage in surface waters was Dorosoma species yolk-sac larvae while the post yolk-sac larvae were more abundant in midwater depth (Table 4-6).

Larval densities also varied by month and closely followed the pattern observed for raw ichthyoplankton counts (Figure 4-7 and Table 4-7). Monthly average densities in March for individual species or fish families were generally low and ranged as high as 0.85/100 m3 for Carp and minnow family post yolk-sac larvae collected at night. A total of five fish families (Catostomidae, Centrarchidae, Clupeidae, Cyprinidae, and Moronidae) had ichthyoplankton life stages collected in March. All these families were collected during nighttime hours but only post yolk-sac larvae of Dorosoma species were collected during daylight. A general pattern observed throughout these collections was that nighttime numbers of collected individual species or fish families and nighttime densities typically exceeded those observed during daylight hours. The only egg collected throughout this six-month survey belonged to a Dorosoma species collected in March at night. The only occurrence of Black Crappie and Largemouth bass ichthyoplankton from the Centrarchidae family was in March.

Ichthyoplankton densities in April were as*high as 15.90/100 m3 for Dorosoma species post yolk-sac larvae collected at night. A total of four fish families (Catostomidae, Clupeidae, Cyprinidae, arid Moronidae) had ichthyoplankton life stages in April. Densities were generally higher in April compared to March. The numbers of collected individual species or fish families was comparable between Day and Night sampling though densities were generally higher at night. The only exception was Quillback whose yolk-sac and post yolk-sac larvae were only collected during daylight. The only occurrences of Chubsucker species and Quillback larvae, family Catostomidae, were in March and April.

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v.c. SUMMER NUCLEAR STATION ENTRAINMENT STL/D.Y-2016 AND REVISED 2017 Ichthyoplankton densities in May were as high as 19.55/100 m3 for Dorosoma species post yolk-sac larvae collected at night. Fish from three families (Centrarchidae, Clupeidae, and Cyprinidae) were collected in May. All these individual species or families were collected during nighttime hours but only yolk-sac and post yolk-sac larvae of Dorosoma species were collected during daylight. In all instances during May, nighttime densities exceeded those measured during daytime. The first collections of Lepomis species (i.e., sunfish) occurred in May.

Ichthyoplankton densities were highest in June with Dorosoma species yolk-sac larvae at night (97.04/100 m3l being the most abundant species and life stage. A total of three fish families (Centrarchidae, Clupeidae, and Ictaluridae) had ichthyoplankton life stages in June. All these families were collected during nighttime hours but only yolk-sac and post yolk-sac larvae of Dorosoma species and post yolk-sac larvae of Lepomis species were collected during daylight. Nighttime densities in June exceeded daytime densities for all species and life stages. The first ictalurid species, Blue Catfish young-of the-year, was collected in June.

Ichthyoplankton densities in July for collected individual species or families were as high as 10.85/100 m3 for Lepomis species post yolk-sac larvae collected during the day. Fish from three families (Centrarchidae, Clupeidae, and Ictaluridae) were collected in July. All these individual species or families were collected during both daytime and nighttime hours with the exception of Channel Catfish young-of the-year which were only collected at night. There was no clear trend indicating higher nighttime densities relative to daytime densities in July.

Ichthyoplankton densities' in August for collected individual species or families were as high as 1.12/100 m3 for Lepomis species post yolk-sac larvae collected during the day and were just slightly higher than those observed in March. A total of four fish families (Centrarchidae, Clupeidae, Ictaluridae, and Percidae) were collected in August. All these individual species or families were collected during nighttime hours but only post yolk-sac larvae ofThreadfin Shad and post yolk-sac larvae of Lepomis species were collected during daylight. Lepomis species were collected from May through August, ictalurid species were collected from June through August, and the only collection of a darter species (Percidae family) was in August.

The most abundant family collected was the Clupeidae (Dorosoma species; Gizzard Shad, and Threadfin Shad) and they were collected in all months of the study though densities were low in March and August.

Densities were generally higher during the night. The second most abundant family was the Centrarchidae and they were collected during March (Black Crappie and Largemouth Bass) and the summer months of May through August (the various sunfish species were probably dominated by Bluegill). No federal or state protected species were identified in ichthyoplankton samples and none would be expected based on fish distribution data or the SCDNR Heritage Trust Program list for Fairfield County, SC.

E11trai11me11t Estimates - The estimated number ichthyoplankton entrained accounted for significant variation in diel ichthyoplankton densities near the CWIS, differences in the duration of day and night periods, and the amount of water withdrawn during those day and night periods. The total estimated number of ichthyoplankton entrained by Summer Station d ruing 2016 was 7 8.1 million ichthyoplankton during the night and 27.3 million ichthyoplankton during the day (Figure 4-8, Tables 4-8 and 4-9). The estimated annual entrainment abundance from 1 March through 31 August 2016 was 105.4 million; with the highest entrainment abundance occurring in June being primarily attributed to Doros01na larvae. The lower and upper bias-corrected 95% confidence limits for the annual entrainment abundance were 89.7 Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 8

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V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 million and 117.0 million, which were determined from the 0.3th and 88.451h percentiles of the distribution of 10,000 bootstrap estimates (Figure 4-9).

5.0 DISCUSSION Ichthyoplankton sampling on Monticello Reservoir for the Summer Station Unit 1 CWIS proceeded smoothly and non-conformities in QA audits were not observed. Environmental or meteorological issues to invalidate collection procedures and thus question data did not occur. Taxonomy Laboratory QA guidelines were strictly followed and an ichthyoplankton reference collection has been securely stored in a vault at the Normandeau Larval Fish Taxonomy Lab (Appendix 4). Our interpretation of these findings is that a valid study for the characterization of ichthyoplankton potentially entrained at the Summer Station Unit 1 CWIS was completed. No federal or state protected species were identified in*

ichthyoplankton samples and none would be expected based on past fish collections in Monticello Reservoir, fish distribution data, or the SCDNR Heritage Trust Program list for Fairfield County, SC.

The ichthyoplankton community in 2016 was composed of seven families and these were identical to the families collected in the 2008 - 2009 ichthyoplankton study for Summer Station Units 2 & 3 (Normandeau 2009). That study sampled ichthyoplankton for a 12-month period in the same vicinity of Monticello Reservoir (Figure 2-5) though outside the area of hydraulic influence of the Unit 1 CWIS.

Interestingly, Dames and Moore (1985) list six of these seven families during the initial operational years of Summer Station (1983 and 1984) but make no mention of bullhead or catfish ichthyoplankton collections (Ictaluridae). Their absence is surprising because ictalurid species have been an ongoing component of the Monticello fishery. Ictalurids have always been captured during South Carolina Department of Natural Resources (SCDNR) cove rotenone sampling conducted in 1987, 1988, 1989, 1995, and 1996 (Christie and Stroud 1992; Christie and Stroud 1997). The potential negative impact to the resident fish community from the 1996 invasion of the reservoir by Blue Catfish was noted in 1997 (Christie and Stroud 1997).

Similar to past studies at Monticello Reservoir, members of the Clupeidae, or the herring and shad family, dominated the March through August 2016 ichthyoplankton collections and entrainment estimate. While the Clupeidae family found in the Broad River basin of SC could be comprised of members of the Alosa and Dorosoma genera, the presence of Alosa species is not supported by solid data. Since American Shad (Alosa sapidissima) have no upstream access to Parr Reservoir during their Congaree River - Broad River spawning migrations, no Blueback Herring (A. aestivalis) were collected in multiple Broad River main channel collections by SCDNR (Bettinger et al. 2003), past Blueback Herring collections in the upper basin have been speculated to be misidentifications (Rohde et al. 2009), and Alosa larvae can be distinguished from Dorosoma larvae by dorsal ray counts; the Clupeidae family in Monticello Reservoir has been shown to be solely represented by members of the Dorosoma genus. Threadfin Shad (Dorosoma petenense) and Gizzard Shad (D. cepedianum) larvae are morphologically similar though appropriately sized larvae can be distinguished by vertebral (Bulak.1985) and anal fin ray counts. Prior to the development of those species-specific anal fin ray counts, small clupeids were labeled Dorosoma species for taxonomic accuracy. However, based on the once-per-year spring spawning of Gizzard Shad and the propensity of Threadfin Shad to spawn during spring and achieve a second spawn in June or July, any Dorosoma species larvae found in June or July could appropriately be labeled Threadfin Shad despite any taxonomic ambiguity.

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\\I. C. SUMMER NUCLEAR STATION. ENTRAINMENT STUDY-2016 AND REVISED 2017 Both Threadfin and Gizzard Shad are important prey species found in southern reservoirs and often contribute substantially to fish biomass (Allen and De Vries 1993). However the Threadfin Shad is a non-native, semi-tropical species introduced by fishery managers to supplement forage populations. Threadfin Shad normally reach sexual maturity as yearlings but may mature during their first year and even spawn a few months after hatching (Rohde et al. 2009). Multiple spawnings per summer, their high fecundity (number of eggs per ovary) which can range from 800 to 21,000 depending on age and size of the female, and small maximum size make them an ideal forage species (Johnson 1971; Jenkins and Burkhead 1994; Rohde et al. 2009).

Gizzard Shad typically mature at age 2 or 3. Each female produces 22,400 to 543,900 eggs (Jenkins and Burkhead 1994), depending on age and size, with some studies showing declining fecundity in older fish (Williamson and Nelson 1985). Both Dorosoma species spawn in the spring when water temperature is greater than 15 °C, but Gizzard Shad usually spawn slightly earlier (Netsch et al. 1971). Both species use shallow water habitats for spawning, but Threadfin Shad tend to spawn near the surface while Gizzard

Shad tend to spawn near the bottom (Shelton et al. 1982). Larval Threadfin Shad tend to move offshore and larval Gizzard Shad remain evenly distributed within 50 m of shore (Allen and De Vries 1993). After the larval stage both species are important prey species, but Gizzard Shad quickly grow too large to be consumed by most predators (Noble 1981). Of the two Dorosoma species, Threadfin Shad is more valuable as a prey species.

Threadfin and YOY Gizzard Shad make up the limnetic forage fish community of Monticello Reservoir and many southeastern US reservoirs. In 2016 larval clupeids comprised 86.0% of all ichthyoplankton estimated to be entrained. The stability and consistency of the clupeid forage fish community over recent years is demonstrated by these same species making up 85% of all entrainable larvae collected and in the 2008 - 2009 study for Summer Station Units 2 and 3 (Normandeau 2009).

Dames and Moore (1985) conducted an ichthyoplankton study in Monticello Reservoir in 1983-1984 as part of the original 316(b) demonstration for Summer Station. In that study, the 1984 mean larval density (during seven months of ichthyoplankton collections) was 53.9 larvae/100 m3 at the surface and 11.8 larvae/100 m3 at mid-depth at a station that was a short distance from the 2016 sampling station. These densities exceed those observed in the current study though Threadfin Shad, the dominant species in this study, was not observed in 1983-1984.

In all likelihood, Threadfin Shad populations in Monticello Reservoir would have exhibited high population fluctuations and readily succumbed to cold shock during severe winters prior to the continuous discharge of the Summer Station thermal effluent. In those pre-operational years, repopulation of the reservoir by Threadfin Shad would gradually occur the following spring and summer via FPSS pumpback or bait bucket introduction. Given a succeeding moderate winter, Threadfin Shad populations would be expected to rebound the following year. Unpublished historical heating degree day data from the SCDNR State Meteorologist office in Columbia (received October 2016) indicates that winters in the 1980's were much colder (i.e., more severe) than the 40-year average (calculated from 1976-2015). Thus, the lack of Threadfin Shad larvae observed by Dames and Moore (1985) can be readily explained by cold shock and death during severe winters not typically experienced in their native semi-tropical habitat.

It appears that once Summer Station became an operational base-load generating station and its warm effluents were a consistent winter feature, the stability and consistency of the clupeid fish community was realized. The SCDNR has conducted fishery investigations of Monticello Reservoir since 1987 that have variously included cove rotenone sampling, trap netting, creel surveys, and nighttime meter netting for Summer Station 2016 lchthyo Final Report - 2017 Addilions.docx 2/28/17 10 Normandeau Associates, Inc.

V.C. SUMMER NUCLE_AR STATION ENTRAINMENT STUDY:_ 2016 AND REVISED 2017 ichthyoplankton. Ichthyoplankton sampling in Monticello Reservoir in 1987, 1988, 1989, and 1997 has consistently demonstrated Threadfin Shad to dominate the age O clupeid prey base (Christie and Stroud 1992; Christie and Stroud 1997). These studies also noted that larval densities in Monticello Reservoir were relatively low compared to regional Catawba-W ateree Reservoirs and ascribed this observation to the relative infertility of Monticello Reservoir. High fecundity, multiple spawns per year, and good over-winter survival in thermally enriched waters have generally allowed Threadfin Shad to dominate clupeid forage fish communities. This domination has been documented at Monticello Reservoir for approximately the last three decades in studies from the 1980's and 1990's by SCDNR and Normandeau Associates in 2008 - 2009 and again in 2016.

Impingement data are in agreement with the high abundance ofThreadfin Shad in Monticello Reservoir.

The 2005 -2006 CWIS impingement.study at Summer Station found 50.2 % of all impinged fish to be small Threadfin Shad with most of that impingement confined to the winter period from December through February (Geosyntec 2007). While numbers of impinged fish were not excessive, the sensitivity of some Threadfin Shad to cold winter temperatures was possibly demonstrated in that study. It should be noted that the naturally limited production of phyto-and zoo-plankton during winter, especially in an infertile reservoir, and subsequent malnourishment may also have played a role in the observed impingement.

One fish egg (Dorosoma species) was collected during the 2016 ichthyoplankton sampling. In all likelihood, this egg was dislodged or adhered poorly to shoreline rip-rap in the vicinity of the CWIS and then collected in our plankton tows. The calculated density and six-month entrainment estimate for this egg were 0.21/100 m3 (at night during March) and 100,000 eggs, respectively. In the 2008 - 2009 larval fish study at Summer Station (Normandeau 2009), no fish eggs were collected in any samples, presumably because all resident species have demersal and adhesive eggs that would not normally be found in the water column. Egg entrainment appears to be a negligible concern at Summer Station.

While water temperatures measured during 2016 were not elevated enough to affect larval fish survival at any measured depth near the CWIS, measured DO concentrations showed lowered values in the deeper samples. These lower concentrations are probably indicative of microbially-induced anoxia or hypoxia in the deepest depths ( deeper than were sampled in this study and deeper than strata from which the CWIS pulls water) of Monticello Reservoir with a progressive increase in concentration near surface layers. DO concentrations in mid-depth and deep water samples (potentially encompassing the deeper range of CWIS inflows) were marginal during summer days but far more hospitable during summer nights for ichthyoplankton occurrence (probably owing to FPSS pumpback operations).

The progression of temperature and DO profiles at the three sample depths gave a general overview of wat~r quality changes every approximate half-month during the 2016 ichthyoplankton sampling. These observed values are comparable to more detailed data collected by SCE&G at three Monticello Reservoir locations in 2015 and specifically to the one labelled 'Intake 2' which is closest to the CWIS (SCE&G 2016). That study concluded water quality in Monticello Reservoir is sufficient to support aquatic life.

Based on the long term survival and persistence of the fish community, especially some of the more fragile forage species like Threadfin Shad, that conclusion appears well supported.

Centrarchids were the second most entrained fish family in 2016. The sunfish, bass, and crappie members of this family typical spawn near shore during spring (crappie and bass) and summer (sunfish) with the male constructing a nest and guarding the young (Rohde et al. 2009). Due to this parental care, most entrainment collections of centrarchids are post yolk-sac larvae that have left the nest but still not of Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 11 Normandeau Associates, Inc.

V. C. SUMMER NUCLEAR STATION ENTRAiNMENT STUDY-2016 AND REVISED 2017 sufficient size to escape entrainment. Based on their preference for limnetic habitats as juveniles, Bluegill and Black Crappie are the species most likely to be entrained at the Summer Station CWIS.

Based on the current study, an estimated 105.4 million ichthyoplankton would be entrained per year under typical operating conditions with 95% confidence limits of 89.7 million and 117.0 million. The ichthyoplankton community of Monticello Reservoir is dominated by one family of fish, the Clupeidae.

The highly fecund Threadfin Shad is the sole species in that family that is being consistently entrained.

Given their preference for wan11 water temperatures, their high reproductive capacity, short life span, and ability to quickly recolonize the reservoir, it is anticipated that a large winter kill event of this species would be undetectable one year later. However, a winter kill ofThreadfin Shad is unlikely as the base-load operation of Summer Station would assure the generation of warm thermal effluents during even the severest of winters. Currently, the operation of Summer Station assures the stability of the Threadfin Shad population, and all the Monticello Reservoir fish predators that feed upon it.

Note -Although not required by EPA 316(b) requirements the original entrainment study "V.C. Summer Nuclear Station Entrainment Study-2016. Prepared for: SCANA RFP WK.9622-(2015)" was peer reviewed by Dr. Charles C. Coutant, Aquatic Ecologist. Dr. Coutant provided a detailed review of the report relative to the EPA 316(b) Rule and provided many excellent comments. We have provided a copy of Dr. Coutant's comments and the applicable SCE&G responses (Appendix 6). This report ("V.C.

Summer Nuclear Station Entrainment Study - 2016 and revised 2017. Prepared for: SCANA RFP WK9622-(2015)") has incorporated Dr. Coutant's suggestions.

6.0 LITERATURE CITED Allen, M.S. and D.R. De Vries. 1993. Spatial and temporal heterogeneity oflarval shad in a large irnpoundment. Trans. Arner. Fish. Soc. 122:12070-1079.

Auer, N.A. (editor). 1982. Identification of larval fishes of the Great Lakes Basin with emphasis on the Lake Michigan drainage. Great Lakes Fishery Commission, Ann Arbor, ML Special Publ. 82-3:

744 pp.

Bettinger, J., J. Crane, and J. Bulak. 2003. Broad River Aquatic Resources Inventory Completion Report. Broad River comprehensive Entrainment Mitigation and Fisheries Resource Enhancement Program. South Carolina Department of Natural Resources.

Bulak, J.S. 1985. Distinction oflarval Blueback Herring, Gizzard Shad, and Threadfin Shad from the Santee-Cooper Drainage, South Carolina. The Journal of the Elisha Mitchell Scientific Society 101(3): 177-186.

Christie, R.W. and R.M. Stroud. 1992. Fisheries investigations in lakes and streams -District N.

SCWMRD Study Comp. Rpt. F-11.

Christie, R.W. and R.M. Stroud. 1997. Fisheries investigations in lakes and streams. Freshwater Fisheries District N. SCDNR Annual Progress Report, F-63-3-4.

Dames & Moore. 1985. 3 l 6(b) Demonstration for the Virgil C. Summer Nuclear Station for the South Carolina Department of Health and Environmental Control and the Nuclear Regulatory Commission. Job. No. 5182 108-09.

Fuiman, L.A. 1978. Descriptions and Comparisons of Northeastern Catostomid Fish Larvae. A Thesis Presented to the Faculty of Cornell University. 110 pp.

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V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-:- 2016 AND REVISED 2017 Geosyntec Consultants. 2005. Delineation of the area of hydraulic influence attributable to the Virgil C.

Summer Nuclear Station Cooling Water Intake Structure. Prepared for South Carolina Electric and Gas Company. Kennesaw, GA.

Geosyntec Consultants. 2007. Preliminary report of fish impingement mortality at the Virgil C. Summer Nuclear Station. Prepared for South Carolina Electric and Gas Company. Kennesaw, GA.

Geosyntec Consultants. 2015. Entrainment sampling plan Virgil C. Summer Nuclear Station Unit 1.

Prepared for South Carolina Electric and Gas Company. Kennesaw, GA.

Haddon, M. 2001. Modelling and Quantitative Methods in Fisheries; Chapman & Hall/CRC, New York.

p.406.

Johnson, J.E. 1971. Maturity and fecundity ofthreadfin shad, Dorosoma petenense, in central Arizona reservoirs. Trans. Amer. Fish. Soc. 100 (74-85).

Jenkins, R.E. and N.M. Burkhead. 1994. Freshwater Fishes of Virginia. American Fisheries Society, Bethesda, MD..

Kuehne, R.A. and R.W. Barbour. 1983. The American Darters. University Press of Kentucky, Lexington.

164 pp.

Lee, D.S., C.R. Carter, C.H. Hocutt, R.E. Jenkins, D.E. McAllister, and J.R. Stauffer Jr. 1980. Atlas of North American Freshwater Fishes. North Carolina Biological Survey. 836 pp.

Lippson, A.I. and R.L. Moran. 1974. Manual for identification of early developmental stages of fishes of the Potomac River Estuary. Prepared for the power plant siting program of the Maryland Dept.

Nat. Resour., PPSP-MP-13. 282 pp.

Menhinick, E.F. 1991. The Freshwater Fishes of North Carolina. North Carolina Wildlife Resources Commission. 221 pp.

McGowan, E.G. 1984. An identification guide for selected larval fishes from Robinson Impoundment, South Carolina. Biology Unit, Carolina Power & Light Co. 56pp.

McGowan, E.G. 1988. An illustrated guide to larval fishes from three North Carolina Piedmont Impoundments. Biology Unit, Carolina Power & Light Co. 113 pp.

Netsch, N.G., G.M. Kensh, Jr., A. Houser, and R.V. Kilambi. 1971. Distribution of young gizzard shad in Beaver Reservoir. Amer. Fish. Soc. Special Pub. 8:95-105.

Noble, R.L. 1981. Management of forage fishes in impoundments of the southern United States. Trans.

Amer. Fish. Soc. 110:738-750.

Normandeau Associates, Inc. (Normandeau). 2009. Monticello Reservoir Ichthyoplankton Studies, September 2008 through August 2009. Prepared for South Carolina Electric and Gas Company.

Bedford, NH.

Normandeau Associates, Inc. (Normandeau). 2016.

Quality assurance plan and standard operating procedures for entrainment sampling at Virgil C. Summer Nuclear Station, Jenkinsville, South Carolina. Prepared for South Carolina Electric and Gas Company. Stanley, NC.

Nuclear Regulatory Commission. 2004. Generic environmental impact statement for license renewal of nuclear plants. Supplement 15 regarding Virgil C. Summer Nuclear Station. Final report. U.S.

Nuclear Regulatory Commission, Washington, DC.

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V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Rohde, F.C., R.G.Arndt, J.W.Foltz, and J.M. Quattro. 2009. Freshwater Fishes of South Carolina. The University of South Carolina Press. 430 pp.

Scotton, L.N., R.E. Smith, NS. Smith, K.S. Price, and D.P. de Sylva. 1973. Pictorial guide to fish larvae of the Delaware Bay, with information and bibliographies useful for the study of fish larvae.

Delaware Rep. Series, Vol 7, College Marine Studies, Univ. of Delaware~ 206 pp.

Shelton, W.L., C.D. Riggs, and L.G. Hill. 1982. Comparative reproductive biology ofthe.threadfin and gizzard shad in Lake Texoma, Oklahoma-Texas. Pages 47-51 in C.F. Bryan, J.V. Conner, and F.

M. Truesdale, editors. The Fifth Annual Larval Fish Conference. Louisiana Cooperative Fisheries Research Unit and the School of Forestry and Wildlife Management. Louisiana State University. Baton Rouge.

Simon, T.P. and R.Wallus. 2006. Reproductive Biology and Early Life Hist01y in the Ohio River*

Drainage, Percidae-Perch, Pikeperch and Darters. Volume 4. CRC Press. 619 pp.

South Carolina Electric & Gas Company (SCE&G). 2016. 2015 water quality monitoring report for Monticello Reservoir. SCE&G Environmental Services.

Wallus, R. and T.P. Simon. 2008. Reproductive Biology and Early Life Hist01y in the Ohio River Drainage, Elassomatidae and Centrarchidae. Volume 6. CRC Press. 443 pp.

Warren, M.L. and 11 others. 2000. Diversity, distribution, and conservation status of native freshwater fishes of the southern United States. Fisheries 25(10):7-31.

Williamson, K.L. and P.C. Nelson. 1985. Habitat Suitability Index Models and Instream Flow Suitability Curves: Gizzard shad. Habitat Evaluation Procedures Group, U.S. Fish and Wildlife Service, Fort Collins, CO. Biological Report 82(10.112).

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----~

1!<)()

,~oo 3000 tee1 GeosyntecD consuJumls Figure 2-2.

Site layout for Virgil C. Summer Nuclear Station on Monticello Reservoir, the Fairfield Pumped Storage Facility, and Parr Reservoir (from Geosyntec 2015). lchthyoplankton entrainment collections in 2016 were made from the vicinity of the Unit 1 Intake while mention of previous ichthyoplankton collections in 2008 - 2009 (Normandeau 2009) was from the vicinity of the Units 2 & 3 Intake.

llt:'.A,1111<1~ 'a,f,T[S, l,,\\.......,~,n --u.:.

t"~.

1:..::::::... '

V Cji~~~~~ll~~~AR2~J~ilOf\\

,- * *::* c*

- sKM-003 Figure 2-3.

Plan view of the Summer Station Unit 1 CWIS showing the path of the CWIS flow between the concrete retaining walls (brown) into one of six intake bays and the location of six traveling screens (blue) and three circulating water pumps (SCANA, unpublished data).

Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 16 Normandeau Associates, Inc.

V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY - 2016 AND REVISED 2017 j

CWISFlow Figure 2-4.

Cross-section view of the Summer Station Unit 1 CWIS showing the path of the CWIS flow past the trash rack (red) extending from the bottom of the skimmer wall (brown), 3/8" square mesh traveling screen (blue), and bar grid prior to encountering the circulating water pump (SCA A, unpublished data).

Legend Proposed Transect Locations ~

urws 2 & 3 ln::a:ce Slr.htce Trc1rl!iect

~

Area ot lrJruera- {H}'Cfrault:- 2ol"le ot lnriuence)

Normandeau Study (2009)

~

m 1 1rr.akc MIO*oe~n Transec!

Su1aCE Transect

' A 11S Mt:tm Maximum Ar** of lntluen<ot and PropoMd lchthJopl*nktClft S*mpling Tr*nHCt:J tt.*rVCSNS Unit 1 lntak*

GeosyntecP Figure co S11 tanis J.r.wr,/111 '.!t Figure 2-5.

Maximum area of hydraulic influence (cross-hatched area) derived by ADCP measurement and ichthyoplankton entrainment sampling transects (vertical lines) used in 2016 near the Summer Station Unit 1 Intake (from Geosyntec 2015). Horizontal lines represent transects sampled in 2008 - 2009 (Nonnandeau 2009) for the future Summer Station Units 2 & 3 Intake structure.

Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 17 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Figure 3-1.

Paired bongo nets and flowmeters used to sample ichthyoplankton in Monticello Reservoir (bottom photo courtesy of Fred Heitman).

Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 18 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 20 17 Figure 3-2.

Paired plankton net sampling cups prior to rinsing (top) and the preservation of a composited sample from previous Monticello Reservoir ichthyoplankton studies (top photo courtesy of Fred Heitman).

Summer Station 2016 lchthyo Final Report-2017 Additions.docx 2/28/17 19 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Figure 3-3.

Collection of water quality data during Monticello Reservoir ichthyoplankton studies.

Figure 3-4.

Analyst sorting ichthyoplankton samples collected during Monticello Reservoir ichthyoplankton studies.

Summer Station 2016 lchthyo Final Report-2017 Additions.docx 2/28/17 20 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 800 700 600

"- 500 a,

..c E 400

s 300 z

200 100 0

March April May June July August Month Figure 4-1.

Monthly counts of ichthyoplankton collected within the area of hydraulic influence in Monticello Reservoir near the Summer Station CWIS, March through August 2016.

0.38%

1.60% 0.15%

0.46%

86.12%

D Clupeids

Centrarch ids

Catostomids D lctalurids

Moronids D Cyprinids D Pe rcids Unidentified Figure 4-2.

Familial composition of ichthyoplankton collected within the area of hydraulic influence in Monticello Reservoir near the Summer Station CWIS, March through August 2016.

Summer Station 2016 lchthyo Final Report-2017 Additions.docx 2/28/17 21 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 35 30 u 25 0 -

~ 20

~

QJ 15 C.

E

~ 10 5

0 3/1/2016 Surface T

--Mid-depth T 6 Deep T 4/1/2016 5/1/2016 6/1/2016 7/1/2016 8/1/2016 9/1/2016 Sampling Date Figure 4-3.

Daytime water temperatures measured at three depths during ichthyoplankton sampling in Monticello Reservoir, March through August 2016.

35 30 u 25 0

~ 20 n,

i...

QJ 15 C.

E

~ 10 5

0 3/1/2016 Surface T

-at-Mid-depth T 6

Deep T 4/1/2016 5/1/2016 6/1/2016 7/1/2016 8/1/2016 9/1/2016 Sampling Date Figure 4-4.

Nighttime water temperatures measured at three depths during ichthyoplankton sampling in Monticello Reservoir, March through August 2016.

Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 22 Normandeau Associates, Inc.

V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY - 2016 AND REVISED 2017 12

~

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2 Surface DO - -- M id-depth DO Deep DO 0

3/1/2016 4/1/2016 5/1/2016 6/1/2016 7/1/2016 8/1/2016 9/1/2016 Sampling Date Figure 4-5.

Daytime dissolved oxygen concentrations measured at three depths during ichthyoplankton sampling in Monticello Reservoir, March through August 2016.

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3/1/2016 4/1/2016 5/1/2016 6/1/2016 7/1/2016 8/1/2016 9/1/2016 Sampling Date Figure 4-6.

Nighttime dissolved oxygen concentrations measured at three depths during ichthyoplankton sampling in Monticello Reservoir, March through August 2016.

Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 23 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Depth-averaged Entrainment Density 200.---~~~----,-~~~~-.-~~~---,~~--.---,-~~~~,--~~~--,

("')

E 150 0

0 z 100

£

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50 0 - 0 ay

Night o L --t1:=~2:::=::==:'.:L:::::0:1_-----1 ___

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e,,..,=-aaw 01Mar 01Apr 01May 01Jun 01Jul 01Aug 01Sep Figure 4-7.

Depth-averaged ichthyoplankton density (no./100 m3) for the day and night period. The vertical dashed lines delineate the sample populations for bootstrapping entrainment abundance during high-abundance season between 15 May and 15 July and the combined low-abundance periods at the tails of the time series.

Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 24 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 A)

Day%

D Clupeids

Centrarchids

Catostomids D lctalurids

Moronids Cyprinids D Percids Unidentified B)

Night%

0.3%

0.8%

0.1%

OClupeids

Centrarchids

Catostomids D lctalurids

Moronids 91.0%

D Cyprinids D Percids Unidentified C)

Total%

0.9%

1.0%

1.6%

9.8%

D Clupeids

Centrarchids

Catostomids D lctalurids

Moronids 86.0%

C!I Cyprinids D Percids Unidentified Figure 4-8.

Percent familial composition of estimated A) day, B) night, and C) total ichthyoplankton withdrawn by the V.C. Summer Nuclear Station considering the ichthyoplankton density in Monticello Reservoir near the intake and the amount of water withdrawn by the station, March through August 2016.

Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 25 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 0.14 -----....... ----,---,---,------....... ----,

Median= 1 08. 1 0.12 UCL 95%=1 17.

>, 0.1 O LCL95 %= 89.7

o.08 n=10,000 co

..0 2 0.06 CL 0.04 0.02 0 ~-'-"----i.....1""""""'..i...i.......................i...i.........-.

80 90 100 110 120 130 140 150 160 Annual Entrainment Estimate (Millions)

Figure 4-9.

Estimates of the median and upper and lower 95% confidence limits for the numbers of ichthyoplankton of all taxa and life stages entrained by the V.C. Summer uclear Station annually based on the displayed probability distribution of a bootstrap analysis of 10,000 runs.

Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 26 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Table 3-1. Allocation of calculated V.C. Summer water withdrawal volume (million cubic meters) among sample period based on mean daily proportion of each diet period (based on the calendar) and per sample period for depth-averaged ichthyoplankton density estimates. The plant operated as a base-load generating facility and exhibited negligible variation in flows during this study.

Water Mean Daily Mean Daily Withdrawal Sample Sample Start End Number Proportion of Proportion of Volume Month Period Date Date Date of Days Daylight Night (million m 3) 3 1

18MAR OlMAR 18MAR 18 0.49 0.51 50.368 3

2 30MAR 19MAR 31MAR 13 0.51 0.49 36.378 4

3 13APR OlAPR 15APR 15 0.53 0.47 41.976 4

4 29APR 16APR 30APR 15 0.55 0.45 41.974 5

5 llMAY Ol MAY 15MAY 15 0.57 0.43 41.972 5

6 25MAY 16MAY 31MAY 16 0.59 0.41 44.769 6

7 08JUN OlJUN 15JUN 15 0.60 0.40 41.971 6

8 22JUN 16JUN 30JUN 15 0.60 0.40 41.971 7

9 15JUL OlJUL 15JUL 15 0.60 0.40 41.971 7

10 27JUL 16JUL 31JUL 16 0.58 0.42 44.768 8

11 lOAUG OlAUG 15AUG 15 0.57 0.43 41.971 8

12 24AUG 16AUG 31AUG 16 0.55 0.45 44.768 Summer Station 2016 lchthyo Final Report-2017 Additions.docx 2/28/17 27 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Table 4-1. Month, common name, and counts of ichthyoplankton collected in Monticello Reservoir, March through August 2016. YSL = yolk-sac larvae, PYSL = post yolk-sac larvae, ULS = undetermined larval stage, and YOY = young-of-the-year.

Month Common name Eggs YSL PYSL ULS YOY March Black Crappie 2

Carp and Minnow Family 4

Dorosorna Species 1

1 Golden Shiner 1

Largemouth Bass 1

White Perch 1

Chubsucker Species 1

Unidentified Osteichthyes 2

April Carp and Minnow Family 13 Dorosorna Species 72 101 70 Quillback 2

1 White Perch 2

9 Chubsucker Species 6

May Carp and Minnow Family 3

Dorosorna Species 39 146 8

Gizzard Shad 1

Lepornis Species 3

June Blue Catfish 1

Dorosorna Species 463 186 Lepornis Species 15 Threadfin Shad 2

July Channel Catfish 1

Dorosorna Species 2

Lepornis Species 94 Threadfin Shad 30 5

August Channel Catfish 3

Darter Species 2

Lepornis Species 11 Threadfin Shad 2

Unidentified Osteichthyes 2

2 Total 1

580 640 85 5

Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 28 Normandeau Associates, Inc.

V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY - 2016 AND REVISED 2017 Table 4-2. Date, time, depth (m), temperature (°C), and dissolved oxygen concentration (DO, mg/I) associated with ichthyoplankton sampling in Monticello Reservoir, March through August 2016.

Date Diel I Time Depth Temp DO 03/18/16 day 17:00 0.3 21.4 11.4 6.1 15.0 10.3 12.2 12.8 10.0 03/18/16 night 22:30 0.3 20.9 11.1 6.1 15.2 10.2 12.2 13.0 9.6 03/30/16 day 16:47 0.3 18.8 10.9 12.2 14.9 9.2 24.4 13.0 8.8 03/30/16 night 22:27 0.3 17.4 9.6 12.2 14.8 8.8 24.4 12.2 8.5 04/13/16 day 17:10 0.3 19.2 10.5 7.6 18.0 10.2 15.2 15.6 9.1 04/13/16 night 22:55 0.3 18.4 9.7 7.6 18.0 9.6 15.2 15.7 8.0 04/29/16 day 16:40 0.3 24.5 11.5 6.1 20.4 8.3 12.2 18.4 8.0 04/29/16 night 22:55 0.3 25.0 11.2 6.1 20.3 9.4 12.2 18.6 7.7 05/11/16 day 16:55 0.3 25.0 10.6 7.6 21.0 8.2 15.2 19.3 7.1 05/11/16 night 22:55 0.3 24.6 10.8 7.6 21.2 7.8 15.2 20.2 7.3 05/25/16 day 16:45 0.3 26.1 11.4 7.6 21.8 8.0 15.2 21.2 7.0 05/25/16 night 23:15 0.3 25.6 11.1 7.6 21.8 8.1 15.2 21.2 7.3 06/08/16 day 16:50 0.3 28.2 8.9 7.6 24.8 6.2 15.2 23.1 5.1 Summer Station 2016 lchthyo Final Report-2017 Additions.docx 2/28/17 29 Normandeau Associates, Inc.

V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Table 4-2.

(Continued).

Date Diel Time Depth Temp DO 06/08/16 night 23:15 0.3 28.4 9.8 7.6 24.4 6.0 15.2 23.6 5.5 06/22/16 day 16:17 0.3 28.5 8.7 7.6 26.2 5.1 15.2 25.0 3.8 06/23/16 night 0:05 0.3 28.7 8.9 7.6 26.4 5.9 15.2 25.7 4.8 07/15/16 day 15:57 0.3 30.1 8.3 7.6 28.5 4.3 15.2 27.7 2.9 07/15/16 night 23:22 0.3 29.8 7.7 7.6 29.3 6.7 15.2 28.4 3.9 07/27/16 day 16:05 0.3 31.3 8.7 7.6 29.3 4.6 15.2 28.6 3.0 07/27/16 night 23:15 0.3 30.9 8.6 7.6 29.1 4.0 15.2 28.7 3.0 08/10/16 day 16:26 0.3 30.1 7.3 7.6 29.7 5.9 15.2 29.5 5.9 08/10/16 night 22:39 0.3 30.0 6.9 7.6 29.9 6.4 15.2 29.3 5.0 08/24/16 day 16:15 0.3 31.1 8.5 7.6 29.7 6.2 15.2 29.6 5.7 08/24/16 night 22:48 0.3 30.6 9.1 7.6 30.0 7.0 15.2 29.6 5.9 Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 30 Normandeau Associates, Inc.

V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY - 2016 AND REVISED 2017 Table 4-3. Environmental conditions associated with ichthyoplankton sampling in Monticello Reservoir March th rough August 2016.

Cloud Air_Temp Cover Wind Wind Speed Date Diel Time (OC)

(%)

Precipitation Direction (mph) 03/18/16 day 17:00 25 50-59 none no wind 0-7 03/18/16 night 22:30 19 50-59 none east 0-7 03/30/16 day 16:47 22 30-39 none south 12-16 03/30/16 night 22:27 17 40-49 none south 8-11 04/13/16 day 17:10 22 30-39 none north 0-7 04/13/16 night 22:55 16 30-39 none east 8-11 04/29/16 day 16:40 32 10-19 none no wind 0-7 04/29/16 night 22:55 25 20-29 none south 0-7 05/11/16 day 16:55 30 40-49 none west 8-11 05/11/16 night 22:55 23 1-9 none no wind 0-7 05/25/16 day 16:45 32 20-29 none west 0-7 05/25/16 night 23:15 25 20-29.

none south 0-7 06/08/16 day 16:50 30 1-9 none west 8-11 06/08/16 night 23:15 25 1-9 none no wind 0-7 06/22/16 day 16:17 33 50-59 none west 0-7 06/23/16 night 0:05 29 1-9 none south 0-7 07/15/16 day 15:57 31 60-69 none south 12-16 07/15/16 night 23:22 25 80-89 none south 0-7 07/27/16 day 16:05 34 50-59 none south 8-11 07/27/16 night 23:15 30 10-19 none west 0-7 08/10/16 night 22:39 27 1-9 none east 0-7 08/10/16 day 16:26 26 80-89 light rain east 0-7 08/24/16 day 16:15 34 50-59 none north 0-7 08/24/16 night 22:48 28 30-39 none north 0-7 Wave Height calm light chop light chop light chop calm light chop calm calm calm calm calm calm calm calm calm calm light chop calm calm calm calm calm calm calm Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 31 Normandeau Associates, Inc.

V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Table 4-4. Daily water usage for in-plant needs (million gallons/day) and monthly totals (million gallons/month) through the Summer Station CWIS from March through August 2016. Shaded cells represent ichthyoplankton sampling dates, horizontal lines indicate the cutoff between first-half and second-half of the month for entrainment calculations.

Month Day March April May June July August 1

0.50076 0.57126 0.49524 0.61782 0.39624 0.46122 2

0.53742 0.59454 0.510068 0.49404 0.39522 0.48216 3

0.52836 0.58446 0.52848 0.54834 0.40126 0.47844 4

0.5388 0.57294 0.45786 0.64164 0.48378 0.46284 5

0.53646 0.67398 0.49386 0.36228 0.4866 0.45954 6

0.51054 0.4632 0.50886 0.41922 0.47454 0.46614 7

0.50514 0.55968 0.49896 0.44052 0.48138 0.45942 8

0.51018 0.54162 0.41088 0.43476 0.4686 0.45846 9

0.49362 0.5322 0.44346 0.43452 0.46428 0.4596 10 0.4917 0.5364 0.44352 0.46272 0.45852 0.48378 11 0.46992 0.49554 0.47946 0.40806 0.45216 0.47928 12 0.4908 0.52854 0.48096 0.3876 0.40266 0.4599 13 0.44274 0.43968 0.4629 0.39438 0.44856 0.4545 14 0.46476 0.50352 0.46632 0.39228 0.48438 0.45918 15 0.4491 0.50652 0.48696 0.41256 0.46296 0.39138 16 0.51732 0.50256 0.49512 0.45288 0.40512 0.44628 17 0.51726 0.51888 0.49482 0.46032 0.422 0.4509 18 0.49512 0.5508 0.48852 0.44802 0.42408 0.45456 19 0.498 0.51894 0.41568 0.44496 0.44574 0.44376 20 0.51558 0.5082 0.42384 0.4557 0.44574 0.46014 21 0.50742 0.49974 0.4827 0.45378 0.43626 0.43926 22 0.501 0.4752 0.46914 0.48258 0.4224 0.44646 23 0.52488 0.54228 0.4782 0.36114 0.40542 0.44862 24 0.51474 0.48254 0.47772 0.35766 0.42438 0.43758 25 0.44676 0.59214 0.46956 0.43572 0.43722 0.43176 26 0.50664 0.49728 0.47928 0.48912 0.42738 0.43302 27 0.50238 0.52626 0.47652 0.4737 0.4224 0.429 28 0.52578 0.52926 0.4626 0.48828 0.42936 0.44016 29 0.55122 0.4209 0.4764 0.4896 0.47676 0.40542 30 0.56424 0.39058 0.49314 0.41076 0.4821 0.4557 31 0.59802 0.20946 0.47178 0.47994 Total 15.75666 15.65964 14.46049 13.55496 13.73928 14.0184 Summer Station 201 6 lchthyo Final Report - 2017 Additions.docx 2/28/17 32 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Table 4-5. Daily water usage for circulation water needs (million gallons/minute) and monthly totals (million gallons/month) through the Summer Station CWIS from March through August 2016. Shaded cells represent ichthyoplankton sampling dates, horizontal lines indicate the cutoff between first-half and second-half of the month for entrainment calculations.

Month Day March April May June July August 1

0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 2

0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 3

0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 4

0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 5

0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 6

0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 7

0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 8

0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 9

0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 10 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 11 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 12 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 13 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 14 0.512998 0.512998

'0.512998 0.512998 0.512998 0.512998 15 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 16 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 17 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 18 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 19 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 20 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 21 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 22 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 23 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 24 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 25 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 26 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 27 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 28 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 29 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 30 0.512998 0.512998 0.512998 0.512998 0.512998 0.512998 31 0.512998 0.512998 0.512998 0.512998 Total 22,900.22 22,161.5 22,900.22 22,161.5 22,900.22 22,900.22 Summer Station 2016 \\chthyo Final Report - 2017 Additions.docx 2/28/17 33 Normandeau Associates, Inc.

V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Table 4-6. Mean ichthyoplankton density (no./100 m3), listed in decreasing order of overall density, for each fish taxon, life stage and sampling depth at VC Summer Nuclear Station, averaged over diel and sample periods from 1 March through 31 August 2016. YSL = yolk-sac larvae, PYSL = post yolk-sac larvae, ULS = undetermined larval stage, and YOY = young-of-the-year.

Surface (n=24)

Midwater (n=24)

Overall (n=48)

Taxon Life Stage Mean Mean Mean*

Dorosoma Species YSL 18.24 62.9 2.54 14.7 10.39 44.9 Dorosoma Species PYSL 5.52 19.1 10.23 59.2 7.87 34.0 Lepomis Species PYSL 2.43 8.4 1.41 8.2 1.92 8.3 Dorosoma Species ULS 1.24 4.3 1.36 7.9 1.30 5.6 Threadfin Shad PYSL 0.24 0.8

o.84 4.9.

0.54 2.3 Carp and Minnow Family PYSL 0.48 1.6 0.20 1.2 0.34 1.5 White Perch PYSL 0.16 0.6 0.18 1.0 0.17 0.7 Chubsucker Species PYSL 0.25 0.9 0.00 0.0 0.12 0.5 threadfin Shad ULS 0.09 0.3 0.06 0.4 0.08 0.3 Channel Catfish YOY 0.00 0:0 0.13 0.7 0.06 0.3 Unidentified Osteichthyes PYSL 0.05 0.2 0.06 0.4 0.06 0.3 Black Crappie PYSL 0.07 0.2 0.00 0.0 0.04 0.2 Quillback YSL 0.07

. 0.2

0.00 0.0 0.03 0.1 White Perch YSL 0.03 0.1 0.04 0.2 0.03 0.1 Darter Species YSL 0.00 0.0 0.06 0.4 0.03 0.1 Unidentified Osteichthyes ULS 0.00 0.0 0.06 0.4 0.03 0.1 Gizzard Shad PYSL 0.00 0.0 0.04 0.2 0.02 0.1 Blue Catfish YOY 0.00 0.0 0.04 0.2 0.02 0.1 Dorosoma Species Egg 0.04 0.1 0.00 0.0 0.02 0.1 Golden Shiner PYSL 0.04 0.1 0.00 0.0 0.02 0.1 Quillback PYSL 0.03 0.1 0.00 0.0 0.02 0.1 Largemouth Bass PYSL 0.00 0.0 0.03 0.2 0.02 0.1 Total 28.98 100.0 17.29 100.0 23.13 100.0 Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 34 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY_;_ 2016 AND REVISED 2017 Table 4-7. Mean depth-averaged ichthyoplankton density (no./100 m3 ) by fish taxon, life stage, diel period and month at VC Summer Nuclear Station representative of the period from 1 March through 31 August 2016. YSL = yolk-sac larvae, PYSL

= post yolk-sac larvae, ULS = undetermined larval stage, and YOY = young-of-the-year.

Mar Apr May Jun Jul Aug Taxon Day Night Day Night Day Night Day Night Day Night Day Night Black Crappie PYSL 0.43 Blue Catfish YOY 0.23 Carp and Minnow Family PYSL 0.85 0.82*

1.80 0.62 Channel Catfish YOY 0.20 0.56 Chubsucker Species PYSL 0.21 0.89 0.38 Darter Species YSL 0.38 Dorosoma Species Egg 0.21 PYSL 0.22 4.52 15.90 12.11 19.55 10.22 31.63 0.18 0.18 ULS 13.96 1.69 YSL 1.67 12.47 2.27 6.00 5.21 97.04 Gizzard Shad PYSL 0.23 Golden Shiner PYSL 0.21 Largemouth Bass PYSL 0.18 Lepomis Species PYSL 0.70 0.66 2.70

10.85 6.12 1.12 0.91 Quillback PYSL 0.21 YSL 0.41 Threadfin Shad PYSL 0.46 1.40 4.23 0.19 0.19 ULS 0.74 0.18 Unidentified Osteichthyes PYSL 0.39 0.32 ULS 0.37 White Perch PYSL 0.21 0.65 1.17 YSL 0.21 0.20 Total 0.22 2.70 9.38 45.88 14.37 28.79 16.09 132.07 13.18 10.91 1.30 2.74 Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 3 5 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STA'rlON ENTRAINMENT STUDY-2016 AND REVISED 2017 Table 4-8. Estimated numbers of ichthyoplankton entrained at VC Summer Nuclear Station (millions) by fish taxon, life stage, and diel period summed for all dates from from 1 March through 31 August 2016, considering ichthyoplankton densities near the station and amount of water withdrawn. YSL = yolk-sac larvae, PYSL = post yolk-sac larvae, ULS =

undetermined larval stage, and YOY = young-of-the-year.

Life Day Day Night Night Total Total Taxon

stage (Millions)

(%)

(Millions)

(%)

(Millions)

(%)

Dorosorna Species YSL 4.5 16.6 39.5 50.5 44.0

41.8 Dorosoma Species PYSL 13.8 50.6 23.8 30.5 37.6 35.7 Lepomis Species PYSL 6.4 23.3 3.7 4.7 10.0 9.5 Dorosoma Species ULS 0.0 0.0 5.8 7.5 5.8 5.5 Threadfin Shad PYSL 0.8 2.9 1.7 2.2 2.5 2.4 Carp and Minnow Family PYSL 0.4 1.4 1.2 1.5 1.6 1.5 White Perch PYSL 0.3 1.1 0.6 0.7 0.9 0.8 Chubs~cker Species PYSL 0.4 1.5 0.2 0.3 0.6 0.6 Threadfin Shad ULS 0.4 1.4 0.1 0.1 0.4 0.4 Unidentified Osteichthyes PYSL 0.0 0.0 0.3 0.4 0.3 0.3 Channel Catfish YOY 0.0 0.0 0.3 0.4 0.3 0.3 Black Crappie PYSL 0.0 0.0 0.2 0.3 0.2 0.2 Quillback YSL 0.2 0.7 0.0 0.0 0.2 0.2 White Perch YSL 0.1 0.4 0.1 0.1 0.2 0.2 Darter Species YSL 0.0 0.0 0.2 0.2 0.2 0.1 Unidentified Osteichthyes ULS 0.0 0.0 0.1 0.2 0.1 0.1 Dorosoma Species Egg 0.0 0.0 0.1 0.1 0.1 0.1 Golden Shiner PYSL 0.0 0.0 0.1 0.1 0.1 0.1 Quillback PYSL 0.1 0.3 0.0 0.0 0.1 0.1 Gizzard Shad PYSL 0.0 0.0 0.1 0.1 0.1 0.1 Blue Catfish YOY 0.0 0.0 0.1 0.1 0.1 0.1 Largemouth Bass PYSL 0.0 0.0 0.1

0.1 0.1 0.1 Total 27.3 100.0 78.1 100.0 105.4 100.0 Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 3 6 Normandeau Associates, Inc.

v.c. *suMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Table 4-9. Estimated numbers of ichthyoplankton entrained at VC Summer Nuclear Station (millions) by fish taxon, life stage, and diel period for each month from 1 March through 31 August 2016, considering ichthyoplankton densities near the station and amount of water withdrawn. YSL = yolk-sac larvae, PYSL = post yolk-sac larvae, ULS = undetermined larval stage, and YOY = young-of-the-year..

Mar Apr May Jun Jul Aug Taxon and Life Stage Day Night Day Night Day Night Day Night Day Night Day Night Total Black Crappie PYSL 0.2 0.2 Blue Catfish YOY 0.1 0.1 Carp and PYSL Minnow Family 0.3 0.4 0.7 0.2 1.6 Channel Catfish YOY 0.1 0.2 0.3 Chubsucker PYSL Species 0.1 0.4 0.1 0.6 Darter Species YSL 0.2 0.2 Dorosoma Egg 0.1 0.1 Species PYSL 0.1 2.1 6.0 6.4 7.1 5.1 10.6 0.1 0.1 37.6 ULS 5.2 0.6 5.8 YSL 0.8 4.7 1.1 2.2 2.6 32.6 44.0 Gizzard Shad PYSL 0.1 0.1 Golden Shiner PYSL 0.1 0.1 Largemouth PYSL Bass 0.1 0.1 Lepomis Species PYSL 0.3 0.3 0.9

5.5 2.1 0.5 0.4 10.0 Quillback PYSL 0.1 0.1 YSL 0.2.

0.2 Threadfin Shad PYSL 0.2 0.7 1.5 0.1 0.1 2.5 ULS 0.4 0.1 0.4 Unidentified PYSL 0.2 0.1 0.3 Osteichthyes ULS 0.1 0.1 White Perch PYSL 0.1 0.3 0.5.

0.9 YSL 0.1 0.1 0.2 Total 0.1 1.2 4.3 17.3 7.5 10.4 8.1 44.4 6.7 3.8 0.6 1.0 105.4 Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 3 7 Normandeau Associates, Inc.

V.C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 APPENDICES Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 Normandeau Associates, Inc.

V. C. SUMMER. NUCLEAR STATION ENTRAINMENT STUDY-2016 AND REVISED 2017 Appendix 1.. Geosyntec Consultants.* 2015. Entrainment sampling plan Virgil C. Summer Nuclear Station Unit 1.. Prepared for South Carolina Electric and Gas Company. Kennesaw, GA.

Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 Normandeau Associates, Inc.

Prepared for SCANA Services, Inc.

220 Operation Way, MC C221 Cayce, South Carolina 29033-3701 ENTRAINMENT SAMPLING PLAN VIRGIL C. SUMMER NUCLEAR STATION UNITl SOUTH CAROLINA ELECTRIC AND GAS COMPANY JENKINSVILLE, SOUTH CAROLINA Prepared by Geosyntec <<>

consultants engineers I scientists I innovators 1255 Roberts Boulevard, Suite 200 Kennesaw, Georgia 30144 Project Number GK5356 March 2015

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consultants TABLE OF CONTENTS

1.

INTRODUCTION...................................... :......................................................... 1 1.1 Entrainment Data Collection Requirements................................................ 1 1.2 Proposed Entrainment Sampling Approach................................................ 2

2.

STUDY BACKGROUND............................................... :.................................... 3 2.1 Facility Description...................................................................................... 3 2.2 Source Water Physical Characteristics...............'......................................... 4 2.3 Source Water Fish and Shellfish Community............................................. 5 2.4 Previous Entrainment Studies...................................................................... 6

3.

SAMPLING PLAN.............................................................................................. 8 3.1 Field Methods.............................................................................................. 8 3.2 Laboratory Processing of Samples............................................................ 11 3.3 Data Analysis............................................................................................. 11 3.4 Quality Assurance and Quality Control..................................................... 12

3. 5 Reporting.................................................................................................... 13

4.

REFERENCES CITED...................................................................................... 15 GK5356/GA140629_Entrainment Sampling Plan.docx 03.30.15

TABLE OF CONTENTS (Continued)

LIST OF TABLES Geosyntec t>

consultants Table 1 Ichthyoplankton Sampling Frequencies for Characterizing Entrainment at VCSNS Unit 1 LIST OF FIGURES Figure 1 Site Vicinity Map, Virgil C. Summer Nuclear Station Figure 2 Site Layout, Virgil C. Summer Nuclear Station Figure 3 Monticello Reservoir Bathymetry. in the Vicinity of Unit 1, Virgil C.

Summer Nuclear Station '

Figure 4 Area of Influence Measured at Three Reservoir Elevations at VCSNS Unit 1, 20-21 April 2005 Figure 5 Maximum Area of Influence and Proposed Ichthyoplankton Sampling Transects Near VCSNS Unit 1 Intake GK5356/GA140629 _Entrainment Sampling Plan.docx ii 03.30.15

ADCP CFR CWIS DNA DO EPA FPSF ft Geosyntec m

m m3 MGD MSL Normandeau NRC QA/QC QAPP SCANA Services SCDHEC SCDNR SCE&G VCSNS ACRONYMS AND ABBREVIATIONS Acoustic Doppler Current Profiling Code of Federal Regulations Cooling Water Intake Structure Deoxyribonucleic acid Dissolved oxygen U.S. Environmental Protection Agency Fairfield Pumped Storage Facility Feet Geosyntec Consultants, Inc.

Inches Meters Cubic meters Million gallons per day Mean Sea Level Normandeau Associates, Inc.

Nuclear Regulatory Commission Quality Assurance/Quality Control Quality Assurance Project Plan SCANA Services, Inc.

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consultants South Carolina Department of Health and Environmental Control South Carolina Department of Natural Resources South Carolina Electric & Gas Company Virgil C. Summer Nuclear Station GK.5356/GA140629_Entrainment Sampling Plan.docx iii 03.30.15

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consultants

1.

INTRODUCTION This study plan proposes the data collection methods to be used for sampling fish and shellffsh entrainment at the cooling water intake structure (CWIS) for South Carolina Electric & Gas Company's (SCE&G's) Virgil C. Summer Nuclear Station (VCSNS)

Unit 1. VCSNS Unit 1 is. an existing, nuclear-powered generating facility located on Monticello Reservoir near Jenkinsville in Fairfield County, South Carolina (Figure 1).

Unit 1 operates using a single CWIS located along the shoreline of Monticello Reservoir as part of a once-through cooling water system regulated by. the South Carolina Department of Health and Environmental Control (SCDHEC) under National Pollutant Discharge Elimination System Permit No. SC0030856 (see facility description in section 2.1 of this study plan). Since Monticello Reservoir has been determined by SCDHEC to be part of a "closed-cycle recirculating system," the facility meets the impingement mortality standard. The entrainment sampling proposed in this plan will assist SCDHEC in determining whether VCSNS Unit 1 meets the entrainment standard and whether the facility will be required to submit further information as outlined in 40 CFR § 122.21(r)(9) through (r)(12).

1.1 Entrainment Data Collection Requirements Under the U.S. Environmental Protection Agency's (EPA's) requirements at 40 CFR § 122.2l(r)(9), the owner or operator of an existing facility that withdraws greater than 125 million gallons per day (MGD) actual intake flow must submit to the permitting director an Entrainment Characterization Study that includes a minimum of two years of entrainment data collection.

However, since VCSNS operates a "closed-cycle recirculating system," SCDHEC has the authority to waive requirements under 40 CFR

§ 122.21(r)(9) through (r)(l2), which includes the two-year entrainment study requirement. SCDHEC has requested that SCE&G perfonn a limited entrainment study which would cover March through August of one year. This study plan addresses that study request. The biological data collection must be representative of the entrainment at the intake and include:

Taxonomic identification of fish and shellfish for all life stages to the _ lowest taxon possible; Description of their abundance and their temporal and spatial characteristics; GK5356/GAl40629_Entrainment Sampling Plan.docx 03.30.15

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consultants Characterization of annual, seasonal, and

diel vanat10ns in entraimnent, including variations related to climate and weather differences, spawning, feeding and water column migration; and Identification of any Federal and/or State protected species.

Total annual entrainment for the facility must be estimated, and all assumptions and calculations used must be identified and documented, together with all methods and quality a.ssurance/quality control procedures for data collection and data analysis. The data collection and analysis methods must be appropriate for a quantitative survey.

1.2 Proposed Entrainment Sampling Approach Six months (March through August) of entrainment sampling for ichthyoplankton (fish eggs, larvae, and shellfish) will be conducted in the vicinity of the VCSNS Unit 1 CWIS beginning in 2015 to represent entrainment at the intake during normal operations. Sampling will be conducted in March through August in the course of one year across 2015-2016. These months represent the primary period of reproduction, larval recruitment, and peak abundance as indicated by ichthyoplankton sampling data collected in the same area of Monticello Reservoir in 2008-2009 for Nuclear Regulatory Commission (NRC) licensing of new VCSNS Units 2 and 3. The CWIS for VCSNS Units 2 and 3 is currently under construction along the southern shoreline of the reservoir approximately 1,250 feet (ft) west of the VCSNS Unit 1 intake. The overall physical characteristics of the reservoir and available aquatic habitats are similar between these two areas, and thus, the ichthyoplankton sampling conducted for the VCSNS Units 2 and 3 intake may also be used to support data collected during the proposed study for the VCSNS Unit 1 intake.

Section 316(b) compliance for the new CWIS being constructed for VCSNS Units 2 and 3 has already been implemented under EPA's Phase I regulations addressing CWISs for new facilities. Thus, the CWIS for Units 2 and 3 is not included in this study plan other than for use of the previously conducted entrainment study in developing the entrainment sampling plan for the Unit 1 CWIS.

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consultants

2.

STUDY BACKGROUND 2.1 Facility Description

VCSNS Unit I is a 972.7-megawatt, nuclear-fueled, base-load generating facility.

Unit I uses a cooling water system with a design intake capacity of approximately 533,122 gallons per minute or 768 MGD. It withdraws cooling water from Monticello Reservoir via a single shoreline CWIS located at the south end of the reservoir (Figure 2).

Although the cooling system operates in a "once-through" mode, Monticello Reservoir was constructed for the purpose of serving as part of the cooling water system* (NRC, 2004). The use of Monticello Reservoir as a cooling impoundment for VCSNS Unit I has been determined to be a "closed-cycle recirculating system" under 40 CFR, Part 125, Subpart J, § 125.92(c)(2).

The VCSNS Unit I CWIS consists of an inlet bay about 550 ft wide east to west and about 200 ft in length north to south (Figure 2). The water depth in the bay ranges from 30 to 40 ft. Bathymetry of the intake bay measured using acoustic Doppler current profiling (ADCP) techniques is presented in Figure 3.

The circulating water intake structure is 93 ft wide with six intake bays each approximately 13-ft wide. Parallel concrete retainer walls extend out into the intake bay of the reservoir a distance of approximately 30 ft. Trash racks comprised of steel bars with IO-inch (in) spacing are located along the upstream face of the intake structure to prevent large debris from entering the intake bays. The trash racks are mounted to the bottom of a skimmer wall that extends from the water surface to a depth of9.5 ft (415.5 ft mean sea level [MSL NGVD29]) at nonnal high water (425 ft MSL). The skimmer wall is designed to exclude floating debris from entering the cooling water system and, combined with the intake retainer walls, to optimize withdrawal of the coolest water from the water column at the pump house. Vertical traveling water screens are located 25 ft behind tl:le trash racks to strain out smaller debris. A bar grid structure is located.

between the traveling screens and the circulating pumps.

Three circulating water pumps convey screened flow to the condensers. At normal high water, the CWIS is designed to withdraw w*ater from the water column between the 415.5 ft and 390 ft MSL; or from a depth of 9.5 ft to 35 ft.

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consultants After leaving the condensers, the heated cooling water discharges to Monticello Reservoir via a 1,000-foot-long discharge canal located east of the CWIS beyond the service water pond and jetty (Figure 2).

2.2 Source Water Physical.Characteristics Monticello Reservoir is a 6,500-acre freshwater impoundment with 51 miles of shoreline. It was built (completed in 1978) to serve as the upper pool for the Fairfield Pumped Storage Facility (FPSF) and the cooling water source for VCSNS Unit 1 (NRC, 2004).

The FPSF generates power by releasing water from Monticello Reservoir into Parr Reservoir, the lower pool. Parr Reservoir is a freshwater impoundment of the Broad River (Figure 1 ). During off-peak power demand periods, FPSF turbines reverse flow and pump water from Parr Reservoir into Monticello Reservoir. Parr Reservoir covers an area of 4,398 acres. The FERC-licensed full-pool elevation of Monticello Reservoir is 425 ft MSL. Monticello Reservoir experiences daily fluctuations in surface elevation of up to 4.5 ft due to pumped storage operations. Monticello Reservoir is deep (average depth of 59 ft; maximum depth of 125 ft) and has a watershed area of only 1,100 ac with little natural surface water inflow.

A survey to delineate the area of hydraulic influence attributable to the VCSNS Unit 1 CWIS was performed in April 2005 using ADCP technology (Geosyntec Consultants, Inc. [Geosyntec], 2005). Intake flow was at or near 739 MGD throughout the survey period. The survey included three hydraulic data collection events conducted over a 24-hour period to represent daily changes in reservoir elevations that occur as a result of FPSF operations. Boundaries of the area of influence at the three reservoir elevations were conservatively estimated (Figure 4) based on the detection of any flow vector oriented towards the CWIS regardless of the associated velocity (ichthyoplankton may be susceptible to entrainment ev:en at low velocities directed toward the CWIS). The overall boundary of the area of influence was delineated to encompass the three areas of hydraulic influence measured at the different reservoir elevations.

The survey delineated a maximum area of influence of 2.92 surface acres, extending from the intake structure approximately 550 ft out into the reservoir with a width of about 250 ft (Figure 5).

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consultants 2.3 Source Water Fish and Shellfish Community The fish community in Monticello Reservoir includes about 29 freshwater species in 7 families, mostly sunfishes (11 species), catfishes (6 species), and suckers (4 species).

Two species of crustaceans have also been documented in the reservoir. A 2007 fish community assessment found Monticello Reservoir to be dominated by three species:

bluegill (Lepomis macrochirus [32.6 percent]), gizzard shad (Dorosoma cepedianum

[(19.6 percent)], and blue catfish (Ictalurus furcatus [11 percent]) (Normandeau Associates, Inc. [Normandeau], 2007). Relative abundance data from another recent community assessment (Normandeau, 2009a) included the following species ranked in descending order of abundance: bluegill (33.4 percent), gizzard shad (6.7 percent),

white perch (Marone americana [21.5 percent]), largemouth bass (Micropterus salmoides [7.6 percent]), and channel catfish (Ictalurus punctatus [5.6 percent]).

Data from previous impingement studies at VCSNS Unit 1 and ichthyoplankton studies in Monticello Reservoir indicate similar species composition. The dominant species from the impingement study performed in 2005-2006 included: threadfin shad (Dorosoma petenense [50.2 percent]), blue catfish (12.2 percent), channel catfish (11.8 percent), white perch (9.4 percent), yellow perch (Perea jlavescens [6.1 percent]), and gizzard shad ( 4.4 percent) (Geosyntec, 2007). Other fish included species from the minnow and sucker families.

The greatest numbers of fish species inhabiting Monticello Reservoir spawn during the period April through June, although there is substantial temporal overlap of spawning periods from as early as March to as late as July and August (Rohde et al., 2009). Early spring spawners include black crappie, gizzard shad, and yellow perch. Mid-spring to early summer spawners include threadfin shad, minnows, suckers, catfishes, and most sunfishes. Gizzard shad and bluegill spawn through the summer.

Crustacean taxa collected in impingement sampks included freshwater grass shrimp (Palaemonetes sp.) and unidentified crayfish (Geosyntec, 2007). No commercially important species of shellfish are known to inhabit Monticello Reservoir.

Based on review of protected species lists for Fairfield County, there are no known occurrences of federally protected threatened or endangered fish or shellfish species, or designated critical habitat for these species, in Monticello Reservoir (South Carolina Department of Natural Resources [SCDNR], 2014; U.S. Fish and Wildlife Service, GK5356/GA140629_Entrainment Sampling Plan.docx 5

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consultants 2014). In March 2008, two specimens of robust red.horse (Moxostoma robustum), a species of conservation concern, were collected in Monticello Reservoir during a largemouth bass survey and are believed to have been entrained into the reservoir through pump-back operations at FPSF, after having been stocked as juveniles upstream in the Broad River (SCDNR, 2013). The robust red.horse inhabits mainstem rivers and occurs in the Broad River primarily downstream of the Neal Shoals Hydro near Carlisle, South Carolina. The species is not listed as protected in South Carolina.

2.4 Previous Entrainment Studies Normandeau (2009b) was contracted to perform an ichthyoplankton survey along the southern shoreline of Monticello Reservoir from September 2008 through August 2009 to assess the potential for entrainment as part of NRC licensing activities for then-proposed VCSNS Units 2 and 3. Ichthyoplankton samples were collected from two transects oriented parallel to the shoreline near the proposed location for the Units 2 and 3 intakes (see Figure 5) using paired (i.e., bongo) 0.5 m-diameter, 0.300 mm-mesh nets.

Samples were collected at the surface along the nearshore transect and at mid-depth along the offshore transect. The transects were located west and in the vicinity of the VCSNS Unit 1 intake. The nets were fitted with calibrated flow meters and each side filtered a minimum of 50 cubic meters (m3) of water in a tow length of 250 m. Samples collected from each net were composited and preserved in the field for subsequent laboratory analysis.

Samples were collected once per month from August through February and twice per month from March through July. Day and night samples were collected during each event. A total of 68 samples were collected during the study.

Organisms were identified to the lowest practical taxon and enumerated as to lifestage, either egg, yolk-sac larvae, post yolk-sac larvae, young-of-year, or juvenile (age 1).

Subsampling was not required for any species or life stage.

Although sampling was conducted year-round, fish larvae were found in samples only from March through August (Nonnandeau, 2009b). No shellfish were collected. The primary species collected in the ichthyoplankton samples as larvae were threadfin shad, which comprised 70.6 percent of the mean monthly sample density, white perch, other

. unknown shads, and gizzard shad. No eggs were collected in any sample, presumably because most resident species have demersal, adhesive eggs typically not found in the water column (Normandeau, 2009b). As a group; the clupeids (shads) comprised 84.7 percent of ichthyoplankton near the intake structure, with the remaining 15.3 percent being comprised of white percli and unidentified minnows and suckers. Historical GK5356/GA140629_Entrainment Sampling Plan.docx 6

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consultants ichthyoplankton studies found similar species composition, with clupeids dominating the samples (Dames & Moore, 1985).

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consultants

3.

SAMPLING PLAN This sampling plan provides for six months of entrainment data collection to consist of ichthyoplankton sampling within the area of influence of the VCSNS Unit 1 CWIS.

This new sampling, along with the previous ichthyoplank:ton study conducted by Normandeau (2009b) in the same vicinity in 2008-2009 may be adequate for documenting potential entrainment at the Unit 1 intake for the purposes of the Entrainment Characterization Study under 40 CPR § 122.21(r)(9).

The following sections describe. the field and laboratory methods, data analysis, and quality assurance/quality (QA/QC) procedures to be followed in implementing the sampling program. This plan proposes methods and sampling frequencies comparable to those used in the 2008-2009 ichthyoplank:ton study.

3.1 Field Methods Ichthyoplankton sampling will be performed in Monticello Reservoir within the area of influence of the Unit 1 CWIS to represent current potential entraimnent at the intake.

Sampling will be conducted twice per month from March through August in the course of one year (Table 1 ).

The months March through August encompass the primary period of reproduction, larval recruitment, and peak abundance for the fish assemblage in Monticello Reservoir, based on the known life history requirements of resident species and as.documented in the previous ichthyoplank:ton study. Sampling events will be conducted a minimum of 7 days apart. During each sampling event, ichthyoplankton samples will be collected during day and night periods to represent diel variation. Day sampling will be conducted at least 2 hours2.314815e-5 days 5.555556e-4 hours 3.306878e-6 weeks 7.61e-7 months after sunrise and 2 hours2.314815e-5 days 5.555556e-4 hours 3.306878e-6 weeks 7.61e-7 months before sunset.

Night sampling will be conducted at least 2 hours2.314815e-5 days 5.555556e-4 hours 3.306878e-6 weeks 7.61e-7 months after sunset and 2 hours2.314815e-5 days 5.555556e-4 hours 3.306878e-6 weeks 7.61e-7 months before sunnse.

FPSF and VCSNS Unit 1 operational information, as well as reservoir elevation infonnation, will be obtained from* SCE&G in advance of each sampling event to characterize sampling conditions, which will be documented for each sampling event.

In certain instances, entrainment sampling may be scheduled during outages as it may be unavoidable under any reaso.nable pre-set schedule.

Outages are a nonnal operational characteristic that will be considered when extrapolating annual entrainment attributable to operation of the CWIS.

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consultants Table 1 lchthyoplankton S_ampling Frequencies for Characterizing Entrainment at VCSNS Unit 1 Surface Transect Mid-depth Transect Month Day Night Day Night Six Months of Sampling Across 2015-2016:

3 Mar 2

2 2

2 Apr 2

2 2

2 May 2

2 2

2 Jun 2

2 2

2 Jul 2

2 2

2 Aug 2

2 2

2 Total Samples 12 12 12 12 a Sampling will begin after March 2015. Months not sampled in 2015 will be sampled in 2016.

Ichthyoplankton samples will be collected at the surface and mid-depth during both the day and night periods in order to represent that portion of the water column in which species may be susceptible to entrainment at the Unit 1 CWIS.* Samples will be collected by towing paired, 0.5-meter (m) diameter plankton nets (bongo nets) with 300-micrometer mesh and equipped with calibrated flow meters. The paired nets will be towed by boat for a distance of approximately 250 meters (825 ft) along transects oriented perpendicular to the shoreline within the area of influence (Figure 5) until a minimum filtered volume of 50 m3 per net is achieved. The tows will be conducted within the area of influence to the maximum extent practicable in order to represent the ichthyoplankton assemblage potentially entrained by the CWIS.

The sampling transects will target the upper portion of the reservoir water column within the area hydraulically influenced by the Unit 1 CWIS. The surface tows will be conducted along one or more transects oriented perpendicular to the reservoir shoreline between the intake structure and the farthest lake-ward extent of the area of influence (Figure 5). Surface tows along more than one transect within the area of influence niay be needed to achieve the threshold sample volume.. The nets will be towed just below the surface within the top 1 m of the water column. Similarly, the mid-depth tows will be conducted along one or more transects oriented perpendicular to the reservoir shoreline between the intake structure and the farthest lake-ward extent of the area of influence; multiple tows may be needed to achieve the threshold sample volume. The nets will be towed along the mid-depth transect at a depth of approximately 5 m GK.5356/GA140629_Entrainment Sampling Plan.docx 9

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consultants (16.5 ft) below the surface. Depth of the mid-depth tows in relation to the bottom of the reservoir will vary depending on reservoir level, as affected by FPSF operations, and location along the transect with respect to the inlet bay. Mixing from FPSF pump-back operations, typically at night, may also contribute to a more even vertical distribution of ichthyoplankton within the water column. Specific configurations of the surface and mid-depth transects may be adjusted in the field if necessary to achieve the target sample volume within the area of influence while avoiding bottom obstructions.

The samples collected in the two sides of the bongo net will be composited in the field into a single sample for laboratory analysis. Samples from the surface transect and mid-depth transect will be kept separately as two distinct samples for each day and night sampling event.

Observations will be recorded in the field as to the estimated proportions of live and dead organisms prior to preservation. Samples will be preserved

'in the field with 5-percent formalin ( or 95-percent ethanol, depending on the laboratory analysis to be conducted), labeled with both internal and external labels, and recorded on a chain-of-custody form. Upon completion of each 24-hour sampling event, all samples will be packaged and sealed for shipping to the taxonomic laboratory for sorting, identification, and enumeration.

During each day and night sampling event, water temperature (°C) and dissolved oxygen (DO) concentration (milligrams per liter) at the surface, mid-depth, and near the bottom, and air temperature (

0 C) will be measured and recorded. Observations will be recorded in the field as to reservoir level, surface currents or waves, and weather conditions, including cloud cover, precipitation, and wind speed and direction.

Sampling data (e.g., flow-meter readings), field measurements, field notes, and plant_

operating conditions will be documented in the field on standardized field data collection fonns.

Plant operational parameters for VCSNS Unit 1, including number of circulating water pumps operating and condenser inlet water temperature, will be obtained from SCE&G and used in conjunction with the study-specific plant operation information recorded on the data sheets. Daily operation records will be used to describe factors potentially affecting entraimnent at VCSNS Unit 1.

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consultants 3.2 Laboratory Processing of Samples Sample processing will be conducted by a laborat01y with current certification under South Carolina's "State Environmental Laboratory Certification Regulation 61-81" for the taxonomic identification of freshwater fishes, marine/estuarine fishes, and.

ichthyoplankton. The intent of the South Carolina Certification requirement is to assure data submitted to SCDHEC are scientifically valid and defensible. Based on early sample results and the ability to identify taxa to species, SCE&G may require

.subsamples to be preserved in ethanol to allow the use of alternative species identification techniques, such as newly developed deoxyribonucleic acid (DNA) techniques. In the case of DNA-based identification, state laboratory certification may not be available.

The taxonomic laboratory will perform quality control checks on the sample sorting process for 10 percent of all samples processed. A minimum of 90 percent of all organisms must be removed from sample debris in order to pass the quality control check for the sorting procedure. Samples with excessive specimen abundance may be split into subsamples but will need to meet a minimum quota of 200 eggs and larvae within the subsample. Sub-sampling may be required for samples collected during peak ichthyoplankton abundance periods, such as for clupeids. Ichthyoplankton samples will be identified to the lowest practicable taxon and enumerated as to life stage, including egg, yolk-sac larva, post yolk-sac larva, young-of-year, and juvenile.

Length measurements will be recorded for up to 25 representative specimens of each taxon in I

the sample.

Results from sample processing will be provided in an electronic spreadsheet. that includes a list of all taxa and their abundance by life stage (egg, yolk-sac larvae, post yolk-sac larvae, young-of-year, and juvenile).

The taxonomic laboratory will also develop and provide a voucher collection that includes representative specimens for each documented taxon and each of its documented life stages:

3.3 Data Analysis Data from the entrainment samples will be standardized to total number of organisms per 100 m3 of water sampled. Extrapolation of the entrainment rates to an annual total will be calculated using an averaged measured ichthyoplankton density ( organisms per GK5356/GAl40629_Entrainment Sampling Plan.docx 11 03.30.15

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consultants m3) and plant water withdrawal rates (m3 of water per month). The entrainment rate will be the number of organisms per month (or other unit of time).

Time periods will bracket the interval between sampling events and will collectively account for six months of plant operation.

The exact time intervals used for I

extrapolation will depend on actual plant operations but will generally represent approximately semi-monthly intervals. A 95-percent confidence limit will be placed on the annual estimate to account for expected diel, seasonal, and operational variability.

3.4 Quality Assurance and Quality Control Prior to the initiation of entrainment sampling, a Project QA/QC Project Plan (QAPP) will be prepared for the information and analyses required by the entrainment sampling plan.

The QAPP will establish and implement a QA Program to ensure that data collection and analysis meet data quality objectives. QA/QC activities will include two sampling event audits. These will include the initial sampling event and one other event during the spring-early summer period of peak larval abundance (March-June). During each QA/QC sampling event, the task manager will document sampling procedures and data processing performed by the field team members.

Results of each QA/QC sampling event conducted and any associated recommendations will be documented in technical memoranda to SCE&G.

The QAPP will contain, at a minimum, information describing:

Documentati~n and recordkeeping; Training requirements; Standard Operating Procedures;

Sampling event audits; Sampling Handling and Custody; Sample sorting and subsampling procedures; Analytical methods; GK.5356/GA140629_Entrainment Sampling Plan.docx 12 03.30.15

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consultants Instrument inspection, calibration, and maintenance; and Data management.

3.5 Reporting Upon completion of the entrainment sampling, laboratory processing, and data analysis described in this plan, an entrainment sampling study report will be prepared and submitted to SCE&G/SCANA Services, Inc. (SCANA Services) for reyiew. The report will describe the study methods anci results and provide a discussion of the key study findings.. The report will present the entrainment. sampling data, including but not necessarily limited to:

Monthly density of ichthyoplankton (number per 100 m3) by species and life stage.

Average density and percent composition of ichthyoplankton collected across the six months of sampling.

Annual entraimnent estimates by species and life stage based on actual CWIS withdrawal rates for the sampling months.

Complete ichthyoplankton data generated for all sampling events, including monthly ichthyoplankton density data by species, life stage, and day/night samples; monthly larval entrainment estimates by species and life stage; and monthly egg entrainment estimates by species and life stage.

Monthly CWIS operation data used in annual entraimnent extrapolation.

Water temperature and DO data associated with each day and night sampling event.

Ambient environmental conditions associated with each day and night sampling event.

Field data forms documenting sampling data (e.g., bongo net flow rates and tow distances), field measurements, field notes, and plant operation information.

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consultants Summary of entrainment data, including tables and charts depicting entrainment data by event and depth as well as seasonal and diel patterns.

A sampling progress update will be provided to SCE&G/SCANA Services after the 2015 sampling activities. An entrainment sampling study report will be prepared at the end of sampling in 2016. The entrainment sampling report will be prepared in draft and final versions to address or incorporate SCE&G/SCANA Services review comments.

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consultants

4.

REFERENCES CITED

Dames & Moore. 1985. 316(b) Demonstration for the Virgil C. Summer Nuclear Station for the South Carolina Department of Health and Environment and the Nuclear Regulatory Commission. Prepared for South Carolina Electric & Gas, Columbia.

Geosyntec Consultants, Inc. (Geosyntec). 2005. Delineation of the Area of Hydraulic Influence Attributable to the Virgil C. Summer Nuclear Station Cooling Water Intake Structure. South Carolina Electric & Gas Company, Jenkinsville, South Carolina.

Geosyntec Consultants, Inc. (Geosyntec ). 2007. Preliminary report of fish impingement mortality at the Virgil C. Summer Nuclear Station, South Carolina Electric and Gas Company, Jenkinsville, South Carolina. Prepared for South Carolina Electric & Gas Company. May 2007.

Normandeau Associates, Inc. (Normandeau). 2007. Monticello and Parr Reservoirs fisheries surveys: Final report. September 2007.

Nonnandeau Associates, Inc. (Nonnandeau). 2009a. Monticello and Parr Reservoirs fisheries surveys: Final report. April 2009.

Normandeau Associates, Inc.

(Nonnandeau).

2009b.

Monticello Reservoir Ichthyoplankton Studies, September 2008 through August 2009.

Nuclear Regulatory Commission (NRC). 2004. Generic Enviromnental Impact Statement for License Renewal of Nuclear Plants. Supplement 15 Regarding Virgil C. Summer Nuclear Station. Final Report. U.S. Nuclear Regulatory Commission, Washington, DC.

Rhode, F. C., R. G. Arndt, J. W. Foltz, and J. M. Quattro. 2009. Freshwater fishes of South Carolina. University of South Carolina Press, Columbia, South Carolina.*

South Carolina Department of Natural Resources (SCDNR). 2013. Final Performance Report: Robust Redhorse Restoration and Conservation. South Carolina State Wildlife Grant F05AF00015 (T-9). October 1, 2004 through September 30, 2013.

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consultants South Carolina Department of Natural Resources (SCDNR). 2014. South Carolina Rare, Threatened & Endangered Species Inventory (species by county). Accessed at http://www. dnr.state. sc. us/pis/heritage/ county species. select county map on August i5, 2014.

U.S. Fish and Wildlife Service. 2014.. Endangered, candidate, and at-risk species; county listings.

Last updated July 23, 2014.

Accessed at http://www.furs.gov I charleston/EndangeredSpecies County.html.

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FIGURES

Hope Station LEGEND

/\\I_ 10-km (6-mi) radius of V.C. Summer N. Interstates

/'v Major Roads Minor Roads County Boundaries Lakes and Rivers Pa rr Hydro Monticello

.lenklnavllle Site Vicinity Map Virgil C. Summer Nuclear Station Jenkinsville, South Carolina Geosyntec t>

consultants Atlanta, Georgia January 2015 s

Figure 1

0 Pa" Reservoir n

Legend

Unit 1 Cooling Water Intake Units 2 & 3 Cooling Water Intake FPSF Intake I

Source: NRC, 2004 Geosyntec C>

consultants Atlanta, Georgia January 2015 Wastewater Treatment Area 1600 3000 feet c=:====---c:====

0 1500 Site Layout Virgil C. Summer Nuclear Station Jenkinsville, South Carolina Figure 2

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consultants Atlanta, Georgia January 2015 0390 - 400 D3so - 390 370 - 380 360 - 370 350 - 360 340 - 3co 330 - 340 320 - 330 310 - 320 300 - 310 290 - 300 280 - 290 Elevation (feet mean sea level)

Monticello Reservoir Bathymetry in the Vicinity ofVCSNS Unit 1 Jenkinsville, South Carolina Figure 3

LEGEND

~CWIS Monticello Reservoir

* boat transects 0

plant building D

High elevation hydraulic influence boundary Declining elevation hydraulic influence boundary D Low elevation hydraulic influence boundary Reservoir stage high declining low size of HZI 1.71 acres 2.01 acres 2.44 acres Distance (It)

Transect from CWIS T1 20 T2 70 T3 148 T4 281 TS 348 Reservoir Elevation High High Declining Low IMSL + 424.6')

(MSL + 424.3')

(MSL + 420.7')

Velocity Direction Velocity otrDcuon velocity Direction ft/s

°mllgn.N ft/s

magn.N ft/s

°magn.N 0.36 182.9 0.35 180.1 0.45 166.2 0.27 126.6 0.27 171.6 0.34 186.8 0.20 61.5 0.14 192.7 0.26 181.8 0.17 327.9 0.12 220.2 0.12 211.6 0.28 294.3 0.04 217.1 0.06 216.8 TS T4 T2 T1 N

A 0

37.5 75 150 225 300 Feet Area of Influence Measured at Three Reservoir Elevations at VCSNS Unit 1, 20-21 April 2005 Jenkinsville, South Carolina Geosyntec t>

consultants Figure 4

i 0 A j

~

fjl

~

j 8 Legend

~

I, Proposed Transect Locations Units 2 & 3 Intake

1. -

Surface Transect

~

Area of Influence (Hydraulic Zone of Influence)

! *111

1111 Mid-depth Transect D

Monticello Reservoir J Normandeau Study (2009)

~

Unit 1 Intake i

Mid-depth Transect 0

2.92-acre Area of Influence 125 Meters N

A 250 Maximum Area of Influence and Proposed lchthyoplankton Sampling Transects Near VCSNS Unit 1 Intake Jenkinsville, South Carolina Geosyntec t>

Figure consultants s

i -- Surface Transect zL-----------------------------------...L..--------...L..--------.....__-----'

Atlanta, Georgia January 2015

V. C. SUMMER NUCLEAR STATION ENTRAINMENT STUDY - 2016 AND REVISED 2017 Appendix 2.

Normandeau Associates, Inc. 2009. Monticello Reservoir lchthyoplankton Studies, September 2008 through August 2009. Prepared for South Carolina Electric and Gas Company. Bedford, NH.

Summer Station 2016 lchthyo Final Report - 2017 Additions.docx 2/28/17 Normandeau Associates, Inc.

ML19056A415

Text

1.0 INTRODUCTION

1.0 INTRODUCTION

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