ML13289A201

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SQN Annual Water Withdrawal Updates for January 2012
ML13289A201
Person / Time
Site: Sequoyah  Tennessee Valley Authority icon.png
Issue date: 02/09/2012
From: John Carlin
Tennessee Valley Authority
To: Waits D
Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
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{{#Wiki_filter:S58 120209 800 - NPDES CORRESPONDENCE February 9, 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L&C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JANUARY 2012 Enclosed is the January 2012 Discharge Monitoring ReportforSequoyah NuclearPlant. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 of Sequoyah's Environmental staff. / certify under penalty oflawthat this document andallattachments were prepared under my direction orsupervision inaccordance with a system designed to assure that qualified personnel properly gatherand evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, orthose persons directly responsible for gathering the information, theinformation submitted is, tothebest ofmyknowledge andbelief, true, accurate, andcomplete. I am aware that there are significant penaltiesfor submitting false information, including thepossibility of fine and imprisonment forknowing violations. Sincerely,, Jofin'XjCsfrlin Sita-w^g/President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 B. E. Brickhouse, LP 5U-C J. T. Carlin, OPS 4A-SQN G. M.Cook, OPS4A-SQN J. A. Cross, POB 2A-SQN T.R. Markum, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 D. B. Nida, LP 5U-C A. A. Ray, WT11A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1E-M

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 February 9, 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L&C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JANUARY 2012 Enclosed is the January 2012 Discharge Monitoring ReportforSequoyah NuclearPlant. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 of Sequoyah's Environmental staff. / certify underpenalty oflawthat this document and allattachments were prepared under my direction orsupervision in accordance with a systemdesignedtoassure that qualified personnel properly gather andevaluate theinformation submitted. Based on myinquiry of theperson or persons who manage thesystem, orthosepersonsdirectly responsible for gathering theinformation, the information submitted is, tothe bestofmy knowledge andbelief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment forknowing violations.

am 6e President Sequoyah Nuclear Plant Enclosures cc (Enclosures):

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555

PERMITTEE NAME/ADDRESS (Include Facility Name/Location ifDifferent) Name ___ _'A ^TOl^^^l^CLE^RPLANT Address P.O. BOX 2000 aNJEROFRCEOPS^r^SONJ^~~~~~Il lQDp^-^l^JTN_37384 FacHity__ jn^-^QyOY^NUCLEAR PLANT Jxration_ Jl^lIJpiijC^UNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD JfEAR MO DAY To YEAR MO DAY 12 01 01 12 01 31 MAJOR (SUBR01) F - FINAL DIFFUSER DISCHARGE EFFLUENT "* NO DISCHARGE Q NOTE: Read instructions beforecompleting thisform PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 1 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM 1668 Req. Mon. DAILY MAX DAILY MX UNITS 03 MGD 62 DEG C/HR MINIMUM Certify under penalty oflaw that this document and all attachments were prepared under my direction orsupervision in accordance with asystem designed toassure that qualified personnel properly gather and evaluate theinformation submitted. Based onmyinquiry oftheperson or persons who manage thesystem, orthose persons directly responsible for gathering the information, theinformation submitted is, tothebestofmyknowledge and belief, true, accurate and complete. Iam aware that there are significant penalties for submitting false information, including the possibility offine andimprisonment for knowing violations. AVERAGE 0.029 0.1 MOAVG MAXIMUM 26.0 Req. Mon. DAILY MAX 10.6 30.5 DAILY MX DAILY MX 0.045 0/1 DAILY MAX UNITS 04 DEG.C. 04 DEG.C. 04 DEG.C. 19 MG/L Form Approved. OMBNo. 2040-0004 NO. EX FREQUENCY OF ANALYSIS 31/31 CONTI NUOUS 31/31 CONTI NUOUS 31/31 CONTI NUOUS 31/31 CONTI NUOUS 10/31 FIVE PER WEEK 31/31 CONTI NUOUS SAMPLE TYPE RCORDR CALCTD MODELD CALCTD CALCTD CALCTD RCORDR RCORDR GRAB CALCTD CALCTD CALCTD NAME/TITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin Sequoyah Site Vice President TELEPHONE DATE TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. Veliger monitoring data isincluded as anattachment. EPAForm 3320-1 (REV 3/99) Previous editions may be used ice President

OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER.

12 02 YEAR MO Page 1 of 1 08 DAY

Sample Date Mean# of ZM/m3 % Settlers Water Temp. (°C) Sample Date Mean# of Asiatic Clams/m3 Water Temp. (°C) LOCATION SUB LOCATION 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 01/10/2012 0 0 9 01/10/2011 0 9 RCW 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 NOTES: % Gravid Asiatic Clam COLLECTED BY PKS WBE PB WPT CR

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name _ JVA ^EQ^^HJ^CLE^R^LANT Address.. _P.O_BOX2000_ aNJ^RQFRCEOPS^N^QN) SODp]f^.^j^jj4_37384 FacjHty__ JYA.-J3EQU0Y/W NUCLEAR PLANT J^(^on_JH>^iypiLQOUNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 101 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD DAY YEAR MO DAY To YEAR MO 12 01 01 12 01 31 MAJOR (SUBR01) F - FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT

      • NO DISCHARGE [^] ***

FormApproved. OMB No. 2040-0004 PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B

  • 1 0

EFFLUENT GROSS SAMPLE MEASUREMENT Monitoring Not Required

              • 4r 23 PERCENT PERMIT REQUIREMENT 43.2 MINIMUM SEMI ANNUAL COMPOS IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1

0 EFFLUENT GROSS SAMPLE MEASUREMENT Monitoring Not Required 23 PERCENT PERMIT REQUIREMENT 43.2 MIMINUM SEMI ANNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEflTTLE PRINCIPAL EXECUTIVE OFFICER John T. Carlln Sequoyah Site Vice President TYPED OR PRINTED Certify underpenaltyof lawthatthisdocumentandallattachmentswere prepared undermy direction orsupervisioninaccordancewitha system designed to assure thatqualified personnel properly gatherand evaluate the information submitted.Based on my inquiry of the person or persons who manage the system, orthose persons directlyresponsible forgatheringthe information, the information submittedis, to the best of my knowledgeand belief,true,accurate, and complete. Iam awarethat there are significantpenalties forsubmittingfalse information, including the possibilityof fine and imprisonment for knowing violations. COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in January 2012. EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNKWKE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE 423 843-7001 AREA CODE NUMBER DATE 12 02 YEAR MO Page 1 of 1 08 DAY

PERMITTEE NAME/ADDRESS (IncludeFacilityName/LocaUonifDifferent) Name _ JVA ^EQ^^HJ^CLEAR_PLANT Address^ _P.0_BOX2000 aNTERQFFlCEOPS^N^QN) SODDY-DAISYJTN_37384 FacjNty^ _JA^-J[EQUOY^ Lgcatjpn_ jj^iypiLQOUNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 103 G PERMIT NUMBER DISCHARGE NUMBER YEAR I MONITORING PERIOD MO DAY To YEAR MO DAY 12 01 01 12 01 31 MAJOR (SUBR01) F - FINAL LOW VOL. WASTE TREATMENT POND EFFLUENT

      • NO DISCHARGE \\^\\ ***

FormApproved. OMB No. 2040-0004 PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH 00400 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT 7 8 12 SU 0 15/31 GRAB PERMIT REQUIREMENT 6 MINIMUM 9 MAXIMUM THREE/ WEEK GRAB SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT 6 6 19 MG/L 0 2/31 GRAB PERMIT REQUIREMENT 30 MO AVG 100 DAILY MX TWICE/ MONTH GRAB OIL AND GREASE 00556 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT <6 <6 19 MG/L 0 2/31 GRAB PERMIT REQUIREMENT 15 MOAVG 20 DAILY MX TWICE/ MONTH GRAB FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT 1.077 1.187 03 MGD ft*0rtF$rttftHtit 4f& Aft AA4f& 0 31/31 RCORDR PERMIT REQUIREMENT Req. Mon. MOAVG Req. Mon DAILY MX SEE PERMIT RCORDR SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED Certify under penalty oflawthatthisdocument andall attachments wereprepared under my direction orsupervision inaccordance with a systemdesigned toassurethatqualified personnel properly gather andevaluate the information submitted. Basedon myinquiry ofthe person or personswhomanagethe system, orthose personsdirectly responsibleforgathering the information, the information submitted is, tothebestofmyknowledge andbelief, true, accurate, andcomplete. Iamaware thattherearesignificant penalties for submitting falseinformation, including the possibility of fineand imprisonment forknowing violations. COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNATUrTOF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE 423 843-7001 AREA CODE NUMBER DATE 12 02 YEAR MO Page 1 of 1 08 DAY

PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) Name _ JVA ^EQ^^^NJJCLE^RJP^NT Address^ _P.O_BOX2g00_ (LNIEROFRCEOPSIN^QN) SODj^-^lSYJlN_37384 FacJlity__ _TW_-SEQUOYAH NUCLEAR PLANJ j^c^on_jH^iypiLCQyi£rY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT FormApproved. OMB No. 2040-0004 TN0026450 110 G PERMIT NUMBER DISCHARGE NUMBER From NO DISCHARGE XX PARAMETER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 01 01 12 01 31 QUANTITY OR LOADING NOTE: Read instructionsbefore completing this form. TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS VALUE TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS VALUE TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM Req. Mon. DAILY MX DAILY MX UNITS 03 MGD 04 DEGC MINIMUM NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify underpenalty oflawthatthisdocument and allattachments werepreparedundermy direction orsupervision inaccordancewith a systemdesignedto assure thatqualified personnel properlygather and evaluatethe information submitted. Based on myinquiry ofthe person or persons whomanage the system,or those persons directly responsibleforgatheringthe information, the information submittedis, to the best ofmyknowledge and belief, true,accurate, and complete. Iamawarethatthere are significant penaltiesforsubmitting false information, includingthe possibility offineand imprisonment for knowing violations. John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used QUALITY OR CONCENTRATION AVERAGE 0.1 MOAVG MAXIMUM REPORT DAILY MX 30.5 DAILY MX DAILY MX 0.1 DAILY MX UNITS 04 DEGC 04 DEGC 04 DEGC 19 MG/L NO. EX TELEPHONE fee President injure of principal executive offTcer or authorized agent 423 843-7001 area CODE NUMBER FREQUENCY OF ANALYSIS CONTIN UOUS CONTIN UOUS CONTIN UOUS CONTIN UOUS Five per Week CONTIN UOUS SAMPLE TYPE CALCTD CALCTD CALCTD RCORDR CALCTD CALCTD DATE 12 02 08 YEAR MO DAY Page 1 of 1

PERMITTEE NAME/ADDRESS (IncludeFacilityName/Location if Different) Name __JVA -SEQUOWHJW^ Address_ ^.O^BOX2pop_ (LNTCROFRCEOPSIN^QN) SQDD]L-^1SYJ1N_37384 Facjlity__JD^-^QUOY^NyCLEAR PLANJ Location. Jl^iypiLQQUNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 110 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD DAY YEAR MO DAY To YEAR MO 12 01 01 12 01 31 MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT

      • NO DISCHARGE

[xx] *** NOTE: Read instructions before completingthis form. FormApproved. OMB No. 2040-0004 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 0 EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM UNITS MINIMUM 43.2 MINIMUM 43.2 MINIMUM NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify underpenalty of lawthatthisdocument andall attachments wereprepared undermy direction orsupervision inaccordance witha system designedto assurethatqualified personnel properly gatherand evaluatethe information submitted. Basedon my inquiry of the personor personswho managethe system, orthose personsdirectly responsibleforgathering the information, the information submitted is, to the best of my knowledgeandbelief,true,accurate, and complete. Iam awarethat there are significant penalties forsubmittingfalse information, includingthe possibilityof fine and imprisonmentforknowingviolations. John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AVERAGE MAXIMUM UNITS 23 PERCENT 23 PERCENT TELEPHONE 423 843-7001 AREA CODE NUMBER SEMI ANNUAL COMPOS SEMI ANNUAL COMPOS DATE 12 02 08 YEAR MO DAY Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation ifDifferent) Name _ _'A ^SEQl^^^NJJC^E^R^l^NT Address__ _P.O_BOX2poo^ aNTCRQFR^OPS^r^SQN) ~~ SODr^-^JSYJTN_37384 Facjljty__ _JW_-JEQUOYA^NUCLEAR PLANT Localoi]_Jhi^lLIPiL(^UJ£rY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 118 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 01 01 12 01 31 Form Approved. OMB No.2040-0004 MAJOR (SUBR01) F - FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE ~XX PARAMETER QUANTITY OR LOADING NOTE: Read instructions before completing thisform. QUALITYOR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE OXYGEN, DISSOLVED (DO) 00300 1 0 EFFLUENT GROSS SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS SOLIDS, SETTLEABLE 00545 1 0 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE Req. Mon. MOAVG MAXIMUM Req. Mon. DAILY MX UNITS 03 MGD MINIMUM 2 MINIMUM NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty oflaw that this document and all attachments were prepared under my direction orsupervision in accordance with asystem designed toassure that qualified personnel properly gather and evaluate theinformation submitted. Based onmy inquiry oftheperson or persons who manage thesystem, orthose persons directly responsible for gathering the information, theinformation submitted is,tothebestofmyknowledge and belief, true, accurate and complete. Iam aware that there are significant penalties for submitting false information including thepossibility offine andimprisonment for knowing violations. John T. Carlln Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall EPAForm 3320-1(REV 3/99) Previous editions may be used AVERAGE MAXIMUM 100 DAILY MX DAILY MX UNITS 19 MG/L 19 MG/L 25 ML/L TWICE/ WEEK TWICE/ WEEK ONCE/ MONTH ONCE/ BATCH Page 1 of 1 GRAB GRAB GRAB ESTIMA

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT - January 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Jennifer Grubb DATE: 2/6/2012 CONCURRENCES Name R V C N Signature - Comment Date J.L Grubb X 6X^-^2^r- &/l 111, B.M. Love X ^^¥-~^k^^ i*lihz~ J.A. Cross X /\\/AAtLOr(>t\\//sW+> 2-lrhy-INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 120308 800 - NPDES CORRESPONDENCE March 8, 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L&C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR FEBRUARY 2012 Enclosed is the February 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. Ifyou have any questions or need additional information, please contact Brad Love at (423) 843-6714 of Sequoyah's Environmental staff. In a letter to Mr. Vojin Janjic dated December 21, 2011 Sequoyah notified TDEC of upcoming activities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle 18 refueling outage scheduled to begin in October 2012. This project has commenced and to date there has been zero discharge of process water. Sequoyah will continue to update TDEC on this process and report any discharges and associated monitoring in future DMR's. / certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance witha system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiryof the person or persons who manage the system, or those persons directlyresponsible forgathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties forsubmitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely

ident Sequoyah Nuclear Plant Enclosures cc (Enclosures)

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 B. E. Brickhouse, BR 4A-C J. T. Carlin, OPS 4A-SQN G. M. Cook, OPS 4A-SQN J. A. Cross, POB 2A-SQN T.R. Markum, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 D. B. Nida, BR4A-C A. A. Ray, WT11A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1E-M

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 March 8, 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L&C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR FEBRUARY 2012 Enclosed is the February 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 of Sequoyah's Environmental staff. In a letter to Mr. Vojin Janjic dated December 21, 2011 Sequoyah notified TDEC of upcoming activities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle 18 refueling outage scheduled to begin in October 2012. This project has commenced and to date there has been zero discharge of process water. Sequoyah will continue to update TDEC on this process and report any discharges and associated monitoring in future DMR's. / certify under penalty oflaw that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best ofmy knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincj fro President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name __JVA^EQ^^m^CLEARPLANT ^<^SS__P.a_BOX2p00_ UNTERQFRCEOPS^N^QN) SOT^-DAJSYJ1N_37384 Facjnty_ JVA; SE^OYAJ+/-NUCLEAR PLANJ LgcatiOTL. Jd^lUPILQQUJNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 02 01 12 02 29 MAJOR (SUBR01) F - FINAL DIFFUSER DISCHARGE EFFLUENT

      • NO DISCHARGE [^ ***

NOTE: Read instructions before completing this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 1 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM 1486 Req. Mon. DAILY MAX DAILY MX UNITS 03 MGD 62 DEG C/HR MINIMUM AVERAGE WWW'ftJt$t'lt'§t 0.034 0.1 MOAVG MAXIMUM 29.4 Req. Mori. DAILY MAX 11.7 30.5 DAILY MX 5 DAILY MX 0.044 0.1 DAILY MAX UNITS 04 DEG.C. 04 DEG.C. 04 DEG.C. 19 MG/L FormApproved. OMB No. 2040-0004 NO. EX FREQUENCY OF ANALYSIS 29/29 CONTI NUOUS 29/29 CONTI NUOUS 29/29 CONTI NUOUS 29/29 CONTI NUOUS 8/29 FIVE PER WEEK 29/29 CONTI NUOUS SAMPLE TYPE RCORDR CALCTD MODELD CALCTD CALCTD CALCTD RCORDR RCORDR GRAB CALCTD CALCTD CALCTD NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertifyunder penaltyof lawthat this document and all attachments were prepared under my directionor supervision inaccordance witha system designed to assure that qualifiedpersonnel properlygather and evaluate the information submitted.Based on myinquiryof the person or persons who manage the system, or those persons directlyresponsible for gathering the information, the information submitted is, to the best of myknowledgeand belief,true, accurate, and complete. Iam aware that there are significantpenalties forsubmittingfalse information, includingthe possibilityoffine and imprisonmentfor knowingviolations. TELEPHONE DATE John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here) No dosed mode operation. Veiiger monitoringdata is included as an attachment. EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNATORE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER 12 03 YEAR MO Page 1 of 1 08 DAY

Sample Date Mean# of ZM/m3 % Settlers Water Temp. (*C) Sample Date Mean* of Asiatic Clams/m3 Water Temp, fC) LOCATION SUB LOCATION NOTES: % Gravid Asiatic Clam COLLECTED BY 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ JVA ^EQ^^HjgjUCL^F^I^ ^p^^^.O^BOXJOOO. aNIERQF^CEOPS^rlSQN) SODpYrDAlSYJLN_37384 Facj!lty_ JfW.- SEQUOY^NUCLEAR PLANT i^(Mojl_Ji>^l!JPiL(^UNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 101 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 02 01 12 02 29 MAJOR (SUBR01) F - FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT

      • NO DISCHARGE Q

FormApproved. OMB No. 2040-0004 PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT Monitoring Not Required 23 PERCENT PERMIT REQUIREMENT 43.2 MINIMUM SEMI ANNUAL COMPOS IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT Monitoring Not Required 23 PERCENT PERMIT REQUIREMENT 43.2 MIMINUM SEMI ANNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlln Sequoyah Site Vice President TYPED OR PRINTED Certify under penalty oflaw thatthisdocument andallattachments were prepared under my direction orsupervision Inaccordance with a system designed toassurethatqualified personnel properly gatherandevaluate theinformation submitted. Based onmy inquiry oftheperson or personswhomanagethe system, orthosepersonsdirectly responsible forgathering the information, theinformation submitted,is, tothebestofmy knowledge andbelief, true, accurate, andcomplete. Iamaware thattherearesignificant penalties forsubmitting false information, including the possibility offineand imprisonment forknowing violations. COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in February 2012. EPA Form 3320-1 (REV 3/99) Previous editions may be used AM4Uice President OF PRINCIPAL EXECUTIVE OR AUTHORIZED AGENT TELEPHONE 423 843-7001 AREA CODE NUMBER DATE 12 03 YEAR MO Page 1 of 1 08 DAY

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ _'A^rai^^^N^CIJ^RJll^NT Mdres^ J^OJ3OX_20Q0_ (INJETOmCEOPS^NbSQN) SQDDX-^1SYJ:NJ7384 Facjllty_ JT^-^QyOY/W NUCLEAR PLANJ i^(^oji_Jl^lUPiLCQyi£rY_. ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 103 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO I DAY YEAR MO DAY 12 02 l 01 To 12 02 29 MAJOR (SUBR01) F - FINAL LOW VOL. WASTE TREATMENT POND EFFLUENT

      • NO DISCHARGE {^\\ ***

NOTE: Read instructionsbeforecompletingthis form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION PH 00400 1 0 EFFLUENT GROSS SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS OIL AND GREASE 00556 1 0 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE wwwwwwww 1.041 Req. Mon. MOAVG MAXIMUM &Aft4t4tftft& 1.246 Req. Mon DAILY MX UNITS 03 MGD MINIMUM 6 MINIMUM AVERAGE 8 30 MOAVG <6 15 MOAVG MAXIMUM MAXIMUM 8 100 DAILY MX <6 20 DAILY MX UNITS 12 SU 19 MG/L 19 MG/L FormApproved. OMB No. 2040-0004 NO. EX FREQUENCY OF ANALYSIS 13/29 THREE/ WEEK 2/29 TWICB MONTH 2/29 TWICE/ MONTH 29/29 SEE PERMIT SAMPLE TYPE GRAB GRAB GRAB GRAB GRAB GRAB RCORDR RCORDR NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty oflawthatthis document andall attachments were prepared under my direction orsupervision inaccordance with a system designed toassure that qualified persom properly gatherandevaluate theinformation submitted. Based onmy inquiry oftheperson or personswhomanagethesystem, orthosepersonsdirectly responsible forgathering the Information, theinformation submitted is, tothebestofmy knowledge andbelief, true, accurate, andcomplete. Iamaware thattherearesignificant penalties forsubmitting falseinformation, including the possibility offineandimprisonment forknowing violations. TELEPHONE DATE John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used ice President RE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER 12 YEAR 03 MO Page 1 of 1 08 DAY

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name __J'* ^MUOY^IWC^EA^UOT ^tosS_^.O^BOX200p_ aNTEEQFFJCE OPSJ5N45QN) SODDX-DAISYJTL37384 i3CjHty_J3^-^QyOYy^ J^<^on_JH>^IIJPJ^TOUNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 110 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 02 01 12 02 29 MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT

      • NO DISCHARGE

[xx] *** NOTE: Read Instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS VALUE TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS VALUE TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM Req. Mon. DAILY MX DAILY MX UNITS 03 MGD 04 DEGC MINIMUM AVERAGE 0.1 MOAVG MAXIMUM REPORT DAILY MX 30.5 DAILY MX 5 DAILY MX 0.1 DAILY MX UNITS 04 DEGC 04 DEGC 04 DEGC 19 MG/L Form Approved. OMB No. 2040-0004 NO. EX FREQUENCY OF ANALYSIS CONTIN UOUS CONTIN UOUS CONTIN UOUS CONTIN UOUS Five per Week CONTIN UOUS SAMPLE TYPE CALCTD CALCTD CALCTD RCORDR CALCTD CALCTD NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify underpenaltyoflawthatthisdocument and allattachmentswerepreparedundermy direction orsupervision inaccordance with a system designed toassurethatqualified personnel properlygather and evaluate the information submitted. Based on myinquiryof the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submittedis, to the best of myknowledgeand belief,true, accurate, and complete. Iam aware that there are significantpenalties for submitting false information, includingthe possibilityoffine and imprisonmentfor knowingviolations. TELEPHONE DATE John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference allattachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used fe Vice President JRE OF PRINCIPAL EXECUTIVE FICER OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER 12 03 YEAR MO Page 1 of 1 08 DAY

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name _J'*^EQ^^l^r^(^EARPLANT Address^ _P.a.BOX2000. aNTEraFRCEOPS^N^QN) S0Dpx-^]SYjw^7384 £acj!jty_ JVA.- SEQUOYAllNyCLEAR PLANJ Mc^on_JlAMypJLQOyi£rY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 110 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR I MO DAY 12 02 01 12 l 02 29 MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE XX Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 0 EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM UNITS MINIMUM 43.2 MINIMUM 43.2 MINIMUM NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify underpenalty oflawthatthisdocument andallattachments werepreparedundermy direction orsupervision inaccordancewith a systemdesignedtoassure thatqualified personnel properly gatherand evaluatethe information submitted. Based on myinquiry ofthe personor persons whomanage the system,or those persons directly responsibleforgatheringthe information, the information submitted is, tothe best ofmyknowledge and belief, true,accurate, and complete. Iamawarethat there are significant penaltiesforsubmitting false information, includingthe possibilityof fine and imprisonmentfor knowingviolations. John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference allattachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNKIWRE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AVERAGE MAXIMUM UNITS 23 PERCENT 23 PERCENT AREA CODE 843-7001 NUMBER SEMI ANNUAL SEMI ANNUAL COMPOS COMPOS YEAR MO DAY Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name _ _'A^EQl^YAHJW^ ^q^s^^P.O^BOXJOOO. aNTCRQFFICEOPS^I^SQN) SOTr^-^AISYJlN_37384 Facjlity_ JVA_-SEQUOY/W NUCLEAR PLANJ j^<^on_jH>WiljpiL^ ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 PERMIT NUMBER 118 G DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR I MO DAY 12 02 01 12 l 02 29 MAJOR (SUBR01) F - FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE XX FormApproved. OMB No. 2040-0004 NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE OXYGEN, DISSOLVED (DO) 00300 1 0 EFFLUENT GROSS SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS SOLIDS, SETTLEABLE 00545 1 0 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE Req. Mon. MOAVG MAXIMUM itititit'itititit w&kkkxx& Req. Mon. DAILY MX UNITS 03 MGD MINIMUM 2 MINIMUM NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify underpenalty oflawthatthisdocument andall attachments wereprepared undermy direction orsupervision inaccordance witha system designedto assurethatqualified personnel properly gather andevaluate the information submitted. Basedonmyinquiry ofthe person or personswhomanagethe system,orthose personsdirectly responsible forgathering the information, the information submitted is, tothebestofmyknowledge andbelief, true, accurate, andcomplete. Iamaware thattherearesignificant penalties for submitting falseinformation, includingthe possibility of fine and imprisonmentforknowingviolations. AVERAGE John T. Carlin Sequoyah Site Vice President SIGNATftSEJOTPRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond otherthanthatresulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used MAXIMUM 100 DAILY MX 1 DAILY MX resident UNITS 19 MG/L 19 MG/L 25 ML/L TELEPHONE 843-7001 AREA CODE NUMBER TWICE/ WEEK TWICE/ WEEK ONCE/ MONTH ONCE/ BATCH GRAB GRAB GRAB ESTIMA DATE 12 03 08 YEAR MO DAY Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT - February 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Jennifer Grubb DATE: 3/7/2012 CONCURRENCES Name R V C N Signature - Comment Date J.L Grubb X S* £c<&h U^-^xUom 3hlit B.M. Love X ^^~~\\rC~Z>-~- 3f*l'^ <lJ> tjz^- J.A. Cross X IJA*Jfa<^ zlsrlt?- INSTRUCTIONS: Originatorwill determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 120406 800 - NPDES CORRESPONDENCE April 6, 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L&C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR MARCH 2012 Enclosed is the March 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 of Sequoyah's Environmental staff. In a letter to Mr. Vojin Janjic dated December 21, 2011 Sequoyah notified TDEC of upcoming activities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle 18 refueling outage scheduled to begin in October 2012. This project is on going and to date there has been zero discharge of process water. Sequoyah will continue to update TDEC on this process and report any discharges and associated monitoring in future DMR's. / certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiryof the person or persons who manage the system, or those persons directlyresponsible for gatheringthe information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely,,- ^resident Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D.Booker, MOB 1F-WBN B. E. Brickhouse, BR 4A-C J. T. Carlin, OPS 4A-SQN G.M.Cook, OPS 4A-SQN J.A.Cross, POB2A-SQN T.R. Markum, BR4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 D. B. Nida, BR 4A-C A. A. Ray,WT11A-K G.R. Signer, WT6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1E-M

M Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy,Tennessee 37384-2000 April 6, 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L&C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR MARCH 2012 Enclosed is the March 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. Ifyou have any questions or need additional information, please contact Brad Love at (423) 843-6714 of Sequoyah's Environmental staff. In a letterto Mr. Vojin Janjic dated December 21, 2011 Sequoyah notified TDEC of upcoming activities forthe installation of pilings into bedrock to support a crane duringthe Unit 2 Cycle 18 refueling outage scheduled to begin in October 2012. This project is on going and to date there has been zero discharge of process water. Sequoyah will continue to update TDEC on this process and report any discharges and associated monitoring in future DMR's. / certifyunder penalty of law that this document and allattachments were prepared under my direction or supervision in accordance with a system designed to assure that qualifiedpersonnel properly gather and evaluate the information submitted. Based on my inquiryof the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, includingthe possibility of fine and imprisonment for knowing violations. Sincerely,

arlin be President foyah Nuclear Plant Enclosures cc (Enclosures)

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name __JVA -SEQ^^^r^CL^^l^NT Address_ J^QU3QX2gog_ QNTEROFRCE OPS^N^QN) SQDDY.-DA]SYJ1N_37384 Facjilty__ jn^-^QyOY^NUCLEAR PLANJ M(^ojL_JH>^IIJPiLCQUi£rY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 03 01 12 03 31 MAJOR (SUBR01) F - FINAL DIFFUSER DISCHARGE EFFLUENT

      • NO DISCHARGE

\\^J *** NOTE: Read instructionsbeforecompletingthis form. Form Approved. OMB No.2040-0004 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT 31.3 Req. Mon. DAILY MAX 04 DEG.C. TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING SAMPLE MEASUREMENT 18.9 TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 1 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT WWWftW'9CW'W 1450 Req. Mon. DAILY MAX wwAAxAAIf DAILY MX 03 MGD 62 DEG C/HR NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty oflaw thatthisdocument andall attachments were prepared under my direction orsupervision inaccordance with asystem designed toassure that qualified personnt John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED properly gather andevaluate the information submitted. Basedonmyinquiry ofthe person or personswhomanagethe system, orthose personsdirectly responsible forgathering the information, theinformation submitted is, tothebestofmyknowledge andbelief, true, accurate, andcomplete. Iamawarethattherearesignificant penalties forsubmitting falseinformation, includingthe possibility of fine and imprisonmentforknowingviolations. COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here) No closed mode operation. Veliger monitoring data is included as an attachment. 0.028 0.1 MOAVG 30.5 DAILY MX DAILY MX 0.057 0.1 DAILY MAX 04 DEG.C. 04 DEG.C. 19 MG/L TELEPHONE SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER 31/31 CONTI NUOUS 31/31 CONTI NUOUS 31/31 CONTI NUOUS 31/31 CONTI NUOUS 20/31 FIVE PER WEEK 31/31 CONTI NUOUS RCORDR CALCTD MODELD CALCTD CALCTD CALCTD RCORDR RCORDR GRAB CALCTD CALCTD CALCTD DATE 12 04 05 YEAR MO DAY EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

Sample Date Mean# of ZM/m3 % Settlers Water Temp. fC) Sample Date Mean# of Asiatic Clams/m3 Water Temp. (°C) LOCATION SUB LOCATION NOTES: % Gravid Asiatic Clam COLLECTED BY 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name __'A ^TOl^^^l^C^E^R^LANT Address_ _P.a.BOX2000. ONJEROFRCEOPS^IISQN) SQD[^-^1SY1JN_37384 Facjljty__ J[\\^-^QyOY/^NUCLEAR PLANT_ LgcatiQD_JiAMypjLQQUNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 101 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 03 01 12 03 31 MAJOR (SUBR01) F - FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT

      • NO DISCHARGE \\~\\ ***

NOTE: Read Instructions before completing this form. Form Approved. OMBNo. 2040-0004 PARAMETER x-QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT Monitoring Not Required 23 PERCENT PERMIT REQUIREMENT 43.2 MINIMUM SEMI ANNUAL COMPOS IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT Monitoring Not Required 23 PERCENT PERMIT REQUIREMENT 43.2 MIMINUM SEMI ANNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED Certify underpenalty of lawthatthisdocument andallattachments wereprepared undermy direction orsupervision inaccordance witha system designedto assurethatqualified personnel properly gatherandevaluatethe information submitted. Basedon my inquiry ofthe personor personswho managethe system, orthose personsdirectly responsibleforgathering the information, the information submittedis, to the best of my knowledgeand belief,true,accurate, and complete.Iam awarethattherearesignificant penaltiesforsubmitting falseinformation, including the possibilityof fine and imprisonment forknowingviolations. COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here) Toxicity was not sampled in March 2012. EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE NUMBER YEAR MO DAY Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ JV* ^EQ^\\^H_NJK^^I^I^I£r Address_ _£.O_B0X2p00_ aNTCRQFRCEOPS^N^QN) SODT^-r^lSY1JN_37384 FacHity___TVA_-SEQUOYAJ^NUCLEAR PLANJ LocatLqn_ JiAMIJPiLC^UNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 103 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 03 01 12 03 31 MAJOR (SUBR01) F - FINAL LOW VOL. WASTE TREATMENT POND EFFLUENT

      • NO DISCHARGE Q

NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION PH 00400 1 0 EFFLUENT GROSS SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS OIL AND GREASE 00556 1 0 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE 1.088 Req. Mon. MOAVG MAXIMUM "kfckiUcitlcIt 1.214 Req. Mon DAILY MX UNITS 03 MGD MINIMUM 6 MINIMUM AVERAGE 8 30 MOAVG <6 15 MOAVG MAXIMUM 8 9 MAXIMUM 8 100 DAILY MX <6 20 DAILY MX UNITS 12 SU 19 MG/L 19 MG/L FormApproved. OMB No. 2040-0004 NO. EX FREQUENCY OF ANALYSIS 15/31 THREE/ WEEK 2/31 TWICE/ MONTH 2/31 TWICE/ MONTH 31/31 SEE PERMIT SAMPLE TYPE GRAB GRAB GRAB GRAB GRAB GRAB RCORDR RCORDR NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify underpenaltyoflawthatthisdocument and allattachments werepreparedundermy direction orsupervision inaccordancewith a systemdesignedtoassure thatqualified personnel properlygatherand evaluatethe information submitted. Basedon myinquiry ofthe personor persons whomanagethe system,or those persons directly responsibleforgatheringthe information, the information submittedis, to the best ofmyknowledge and belief, true,accurate, and complete.Iam aware that there are significant penaltiesforsubmitting false information, includingthe possibility offine and imprisonment for knowing violations. TELEPHONE DATE John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Sfuoyan Site Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER 12 04 YEAR MO Page 1 of 1 05 DAY

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name __'Al?EQUO^HJ^CLE^R^^NT Address_ JLQJ*OX_2000. aNTERQFRCEOPS^Nj^QN) SODC^-MiSYJlN_37384__. Facjjjty_ JH^-J^QUOY/W MCLEAR PLANJ Jxcation_Jl>^IUPiLQQUjnY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 110 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 03 01 12 03 31 MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT

      • NO DISCHARGE

[xx] *** NOTE: Read instructions beforecompleting this form. FormApproved. OMB No. 2040-0004 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS VALUE TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT WWW'&ftJt'WW FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS VALUE PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT Req. Mon. DAILY MX 03 MGD CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS VALUE PERMIT REQUIREMENT TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT 04 DEGC DAILY MX NAMEmTLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty oflaw that this document and all attachments were prepared under my direction orsupervision inaccordance with a system designed toassure thatqualified personnel properly gather andevaluate the Information submitted. Based onmyinquiry ofthe person or persons whomanage thesystem, orthosepersons directly responsible for gathering the information, the information submitted is, tothe best ofmy knowledge and belief, true, accurate? andcomplete. Iamaware thatthere aresignificant penalties for submitting false information, including the possibility of fineandimprisonment forknowing violations. John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS No Discharge this Period (Reference allattachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AVERAGE 0.1 MOAVG wwwwww $fw MAXIMUM REPORT DAILYMX 30.5 DAILYMX DAILYMX 0.1 DAILY MX UNITS 04 DEGC 04 DEGC 04 DEGC 19 MG/L TELEPHONE ice President 423 843-7001 AREA CODE NUMBER CONTIN UOUS CONTIN UOUS CONTIN UOUS CONTIN UOUS Five per Week CONTIN UOUS CALCTD CALCTD CALCTD RCORDR CALCTD CALCTD DATE 12 04 05 YEAR MO DAY Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name __'A ^EQ^^m^CLE^^LANT jM<^ss__P.01_BOX2gop_ QNTEROFFiCEOPS^rtSON) SOTDY.-DAjSYJT07384_ Facjijty_ JfVA.- SEQyOYi^NUCLEAR PLANJ LocatLqn_ JH/^iypiLCOUNJY.. ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 110 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 03 01 12 03 31 MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT

      • NO DISCHARGE

[xx] *** NOTE: Read instructionsbeforecompletingthis form. FormApproved. OMB No. 2040-0004 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 0 EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM UNITS MINIMUM 43.2 MINIMUM 43.2 MINIMUM NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify under penalty oflaw thatthisdocument andallattachments wereprepared under my direction orsupervision inaccordance with a system designed toassurethatqualified personnel properly gatherandevaluatethe information submitted. Basedonmyinquiry ofthepersonor personswhomanagethe system,or those personsdirectly responsible forgathering the information, the information submitted is, tothebestofmyknowledge andbelief, true,accurate, and complete. Iamawarethatthereare significant penaltiesforsubmitting false information, including the possibility offineand imprisonment forknowing violations. John T. Carlln Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used AVERAGE SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT MAXIMUM UNITS 23 PERCENT 23 PERCENT AREA CODE 843-7001 NUMBER SEMI ANNUAL SEMI ANNUAL COMPOS COMPOS YEAR MO DAY Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ JVA -^(MI^^^I^C^^r^LANT Address^ Jl.OJlgX2p00_ (LNTEROFRCEOPSIN^QN) SODDX-DA1SYJ7137384 Facjilty_ J^-^QUOYM IWCLEAR PLANJ i^catjon_ Ji^iyPILQOUNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 118 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 03 01 12 03 31 Form Approved. OMB No. 2040-0004 MAJOR (SUBR01) F - FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE XX NOTE: Read instructions beforecompleting this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE OXYGEN, DISSOLVED (DO) 00300 1 0 EFFLUENT GROSS SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS SOLIDS, SETTLEABLE 00545 1 0 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE Req. Mon. MOAVG MAXIMUM Req. Mon. DAILYMX UNITS 03 MGD MINIMUM 2 MINIMUM NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify under penalty oflaw thatthisdocument andall attachments were prepared under my direction orsupervision inaccordance with a systemdesigned toassurethatqualified personnel properly gather, andevaluate theinformation submitted. Based onmyinquiry ofthe person or personswhomanage the system,orthose persons directly responsible for gathering the information, the information submitted is, tothebestofmyknowledge andbelief, true, accurate, andcomplete. Iamaware thattherearesignificant penalties for submitting false information, including the possibility of fineand imprisonment forknowing violations. John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS ANDEXPLANATION OFANYVIOLATIONS (Reference allattachments here) During this reporting period, there has been no flow from the Dredge Pond otherthan that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used AVERAGE MAXIMUM 100 DAILYMX DAILYMX UNITS 19 MG/L 19 MG/L 25 ML/L TELEPHONE iVice President SIGNATUREOF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER TWICE/ WEEK TWICE/ WEEK ONCE/ MONTH ONCE/ BATCH GRAB GRAB GRAB ESTIMA DATE 12 04 05 YEAR MO DAY Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT - March 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Jennifer Grubb DATE: 4/5/2012 Name J.L Grubb B.M. Love J.A. Cross R V C N CONCURRENCES Signature - Comment Date 4 lei12.- Hid IZ. VI*ll* INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicableto the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 120510 800 - NPDES CORRESPONDENCE May 10, 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L&C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR APRIL 2012 Enclosed is the April 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. Ifyou have any questions or need additional information, please contact Brad Love at (423) 843-6714 of Sequoyah's Environmental staff. In a letter to Mr. Vojin Janjic dated December 21, 2011 Sequoyah notified TDEC of upcoming activities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle 18 refueling outage scheduled to begin in October 2012. This project is on going and to date there has been zero discharge of process water. Sequoyah will continue to update TDEC on this process and report any discharges and associated monitoring in future DMR's. / certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely Site-vice President Sequoyan Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D. Booker, MOB 1F-WBN B. E. Brickhouse, BR 4A-C J.T. Carlin, OPS 4A-SQN G. M. Cook, OPS 4A-SQN J. A. Cross, POB 2A-SQN T.R. Markum, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 D. B. Nida, BR4A-C A. A. Ray, WT11A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1E-M

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 May 10, 2012 Ms. Dana Waits State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L&C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR APRIL 2012 Enclosed is the April 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 of Sequoyah's Environmental staff. In a letter to Mr. Vojin Janjic dated December 21, 2011 Sequoyah notified TDEC of upcoming activities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle 18 refueling outage scheduled to begin in October 2012. This project is on going and to date there has been zero discharge of process water. Sequoyah will continue to update TDEC on this process and report any discharges and associated monitoring in future DMR's. / certify under penalty oflaw that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the informationsubmitted. Based on my inquiryof the person or persons who manage the system, or those persons directly responsible forgathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties forsubmitting false information, including the possibility of fine and imprisonment for knowing violations. President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555

PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different) Name _ _'A ^EQ^YA^N^CLE^R^l^NT Address, _P.O.BOX2goo. I (LNTEROFRCEOPS^N^QN) SODDX-DAISY^N^7384 Facjljty_ jn^-^QUOY^NUCLEAR PLANJ Localon_ JH/WIITpILQQyMY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD I DAY YEAR MO DAY To YEAR MO 12 04 01 12 04 30 MAJOR (SUBR01) F - FINAL DIFFUSER DISCHARGE EFFLUENT

      • NO DISCHARGE [^] ***

NOTE: Readinstructions before completing thisform PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 S EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE &*kA4>AA4r4t MAXIMUM 1718 Req. Mon. DAILY MAX DAILY MX UNITS 03 MGD 62 DEG C/HR MINIMUM AVERAGE 0.022 0.1 MOAVG MAXIMUM 33.8 Req. Mon. DAILY MAX 22.2 30.5 DAILY MX DAILY MX 0.059 0.1 DAILY MAX UNITS 04 DEG.C. 04 DEG.C. 04 DEG.C. 19 MG/L Form Approved. OMB No. 2040-0004 NO. EX FREQUENCY OF ANALYSIS 30/30 CONTI NUOUS 30/30 CONTI NUOUS 30/30 CONTI NUOUS 30/30 CONTI NUOUS 26/30 FIVE PER WEEK 30/30 CONTI NUOUS SAMPLE TYPE RCORDR CALCTD MODELD CALCTD CALCTD CALCTD RCORDR RCORDR GRAB CALCTD CALCTD CALCTD NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certifyunder penalty of lawthat this document and all attachments were prepared under my directionor supervision in accordance witha system designed to assure that qualifiedpersoni properly gatherandevaluatethe information submitted. Basedonmyinquiry ofthepersonor! persons who manage thesystem, orthosepersons directly responsible forgathering the information,the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TELEPHONE DATE John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS ANDEXPLANATION OF ANYVIOLATIONS (Referenceallattachments here) No closed mode operation. Veliger monitoring dataisincluded as an attachment. Spectrus CT1300 treatment began on4/30/12 (max. calc. cone, was0.037mg/L~limit 0.050mg/L). EPA Form 3320-1 (REV 3/99) Previous editions may be used ice President ature of principal executive Acer or authorized agent 423 843-7001 AREA CODE NUMBER 12 05 YEAR MO Page 1 of 1 09 DAY

Sample Date Mean # of ZM/m3 % Settlers Water Temp. (°C) Sample Date Mean# of Asiatic Clams/m3 Water Temp. (°C) LOCATION SUB LOCATION 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 01/10/2012 0 0 9 01/10/2011 0 9 RCW 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 NOTES: % Gravid Asiatic Clam COLLECTED BY PKS WBE PB WDT CR BB WE CR WBE WBE WBE WBE WBE PB PB MJW

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name _ _'A ^EQ^^HJ^C^E^I^^NT jMdress__P.a.BOX2p00_ aNTERgFRCEOPSJN^QN) SOTE^-^1SY1_TN_37384 FacHjty_ JVA;J^QUOY/W NUCLEAR PLANJ J^(^Oii_Jl>^IIJPiLCOUJirY^ ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 101 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 04 01 12 04 30 MAJOR (SUBR01) F - FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT

      • NO DISCHARGE

[^] *** NOTE: Readinstructions before completing thisform. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 EFFLUENT GROSS IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM UNITS MINIMUM Monitoring Not Required 43.2 MINIMUM Monitoring Not Required 43.2 MIMINUM AVERAGE MAXIMUM UNITS 23 PERCENT 23 PERCENT Form Approved. OMB No. 2040-0004 NO. EX FREQUENCY OF ANALYSIS SEMI ANNUAL SEMI ANNUAL SAMPLE TYPE COMPOS COMPOS NAMEnTTLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personi properly gather and evaluate the information submitted. Based on my inquiryof the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significant penalties for submitting false infonnation, including the possibility of fine and imprisonment for knowing violations. TELEPHONE DATE John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTSAND EXPLANATION OFANY VIOLATIONS (Referenceallattachmentshere) Toxicity was not sampled in April 2012. EPA Form 3320-1 (REV 3/99) Previous editions may be used ite Vice President OF PRINCIPAL EXECUTIVE OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER 12 05 YEAR MO Page 1 of 1 DAY

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name _ JVA-SEQUO^l^NJ£CLE^R^I^NT M*ress__P.Oi_BOX_200JL aNIERQFRCEOPSJItSQN) SODDY.-^ISYaJN_37384 FacjHty_ JV^-^QUOY^ISKiQLEAR PLANJ Juration. Jl^iypjli^UNJY ATTN: Brad Love NATIONALPOLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 103 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR I MO DAY 12 04 01 12 l 04 30 MAJOR (SUBR01) F - FINAL LOW VOL. WASTE TREATMENT POND EFFLUENT NO DISCHARGE NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION PH 00400 1 0 EFFLUENT GROSS SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS OIL AND GREASE 00556 1 0 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE 4r4r4r4r4r4r4r4r 1.127 Req. Mon. MOAVG MAXIMUM 1.268 Req. Mon DAILY MX UNITS 03 MGD MINIMUM 6 MINIMUM AVERAGE 9 30 MOAVG <6 15 MOAVG MAXIMUM MAXIMUM 10 100 DAILY MX <6 20 DAILY MX UNITS 12 SU 19 MG/L 19 MG/L Form Approved. OMB No. 2040-0004 NO. EX FREQUENCY OF ANALYSIS 15/30 THREE/ WEEK 2/30 TWICE/ MONTH 2/30 TWICE/ MONTH 30/30 SEE PERMIT SAMPLE TYPE GRAB GRAB GRAB GRAB GRAB GRAB RCORDR RCORDR NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my Al^yafrSiteVice President TELEPHONE DATE John T. Carlin Sequoyah Site Vice President properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. 1am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. 423 843-7001 12 05 09 SIGNAL[URE OF PRINCIPAL EXECUTIVE CER OR AUTHORIZED AGENT TYPED OR PRINTED AREA CODE NUMBER YEAR MO DAY COMMENTS ANDEXPLANATION OF ANYVIOLATIONS (Referenceallattachmentshere) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name _ J'A ^EQ^^^f^C^^RJPLANT ^9^SS__£.OJtoX_200g_ (LNraTOFRCEOP^r*£<^ SOTC^-^1SYJ1N_37384 Facjlity__ _TM_-SEQUOYJW NUCLEAR PLANJ McatjprL. Jd>^iypjLQQUNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 110 G PERMIT NUMBER DISCHARGE NUMBER From MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE [xx] FormApproved. OMB No. 2040-0004 MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 04 01 12 04 30 NOTE: Read Instructions before completing this form. PARAMETER TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS VALUE TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS VALUE TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT QUANTITY OR LOADING AVERAGE MAXIMUM Req. Mon. DAILY MX 2 DAILY MX UNITS 03 MGD 04 DEGC MINIMUM NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certifyunder penaltyof lawthat this document and allattachmentswere preparedunder my directionor supervision in accordance witha system designed to assure that qualifiedpersonnel properlygather and evaluate the informationsubmitted. Based on my inquiryof the person or persons who manage the system, or those persons directly responsible for gathering the information,the informationsubmitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significant penalties for submitting false information, including the possibilityof fine and imprisonment for knowing violations. John T. Carlin QUALITY OR CONCENTRATION AVERAGE 0.1 MOAVG MAXIMUM REPORT DAILY MX 30.5 DAILY MX DAILY MX 0.1 DAILY MX UNITS 04 DEGC 04 DEGC 04 DEGC 19 MG/L NO. EX TELEPHONE FREQUENCY OF ANALYSIS CONTIN UOUS CONTIN UOUS CONTIN UOUS CONTIN UOUS Five per Week CONTIN UOUS SAMPLE TYPE CALCTD CALCTD CALCTD RCORDR CALCTD CALCTD DATE 423 843-7001 12 Sequoyah Site Vice President TYPED OR PRINTED SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE NUMBER 05 09 YEAR MO DAY COMMENTS ANDEXPLANATION OFANYVIOLATIONS (Reference allattachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name _ JTVA ^M^^HJ^C^^I^I^NT Mdre^Ji.OJ30XjragO_ (LNTCROFRCEOPS^N^QN) SODr^-^lSYJlN_37384 Facjjjty_ J^^-^QUOY^NUCLEAR PLANJ j^(^oiL_Ji>MiUPJ^QQUr£nL-ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 110 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 04 01 12 04 30 MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT

      • NO DISCHARGE

[xx] *** NOTE: Read instructions before completing,this form FormApproved. OMB No. 2040-0004 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 0 EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM UNITS MINIMUM 43.2 MINIMUM 43.2 MINIMUM NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certifyunder penalty of lawthat this document and all attachments were prepared under my direction or supervision in accordance witha system designed to assure that qualified personnel properly gather and evaluate the informationsubmitted. Based on my inquiryof the person or persons who manage the system, or those persons directly responsible for gathering the infonnation, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significant penalties for submittingfalse information, includingthe possibility of fine and imprisonmentfor knowingviolations. John T. Carlln Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNATUBtfOF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AVERAGE MAXIMUM ite Vice President UNITS 23 PERCENT 23 PERCENT TELEPHONE 423 843-7001 AREA CODE NUMBER SEMI ANNUAL COMPOS SEMI ANNUAL COMPOS DATE 12 05 09 YEAR MO DAY Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name __'A ^raUO^^r^CL^R_PLANT ^p^ss_JP.O^BOX2p0g_ aNJEROFFl(^OPS^N-SQN) SQD[^-DAISYJ^7^4 FaclHty_ J]v^-^QyOY>^lsmQLEAR PLANJ jx(^ojLJl>MiUPJLQQyiirY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 118 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 04 01 12 04 30 MAJOR (SUBR01) F - FINAL WASTEWATER & STORM WATER EFFLUENT

      • NO DISCHARGE

[xx] *** NOTE: Read instructions before completing this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION OXYGEN, DISSOLVED (DO) 00300 1 0 EFFLUENT GROSS SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS SOLIDS, SETTLEABLE 00545 1 0 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE Req, Mon. MOAVG MAXIMUM AtirftAAAfAfA Req. Mon. DAILY MX UNITS 03 MGD MINIMUM 2 MINIMUM AVERAGE MAXIMUM 100 DAILY MX DAILY MX UNITS 19 MG/L 19 MG/L 25 ML/L Form Approved. OMB No. 2040-0004 NO. EX FREQUENCY OF ANALYSIS TWICE/ WEEK TWICE/ WEEK ONCE/ MONTH ONCE/ BATCH SAMPLE TYPE GRAB GRAB GRAB ESTIMA NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify under penalty of law that this document and all attachments were prepared under my directionor supervision inaccordance witha system designed to assure that qualifiedpersonnel properly gather and evaluate the infonnation submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TELEPHONE DATE John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTSAND EXPLANATION OF ANYVIOLATIONS (Reference all attachmentshere) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Site Vice President OF PRINCIPAL EXECUTIVE OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER 12 05 YEAR MO Page 1 of 1 09 DAY

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT-April 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 5/4/2012 CONCURRENCES Name R V C N Signature - Comment Date B.M. Love X tf^^- sN1^ L.M. Koby X "Sl'S/lz-J.A. Cross X MUM>(A/tUp* ' <rhl> >- INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 120612 801 - NPDES CORRESPONDENCE June 12, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L&C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR MAY 2012 Enclosed is the May 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. On May 24, 2012 at 15:27 Eastern Daylight Time (EDT) visible oil sheen was identified on the east side of the essential raw cooling water (ERCW) intake building at SQN on the Tennessee River. The source of the oil sheen was caused by an oil drip pan overflow located at the C-B ERCW traveling water screen gear drive. The leak was immediately secured and the discharge was stopped. The estimated quantity of oil discharged is less than one gallon. Because the oil spill occurred in a low flow area and favorable meteorological conditions (light winds, no rain), the water was fairly stagnant and the sheen was easily contained and cleaned up utilizing absorbent booms and pads. TVA provided oral notification to Angela Young of the Chattanooga Environmental Field Office via voice message on May 24, 2012. Documentation of the incident and corrective actions are being performed in accordance with the site's Corrective Action Program. In a letter to Mr. Vojin Janjic dated December 21, 2011 SQN notified TDEC of upcoming activities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle 18 refueling outage scheduled to begin in October 2012. This project is complete and resulted in the discharge of approximately 700 gallons of water through the SQN low volume waste treatment process. The results for the pH and TSS collected during discharge have been incorporated into the DMR. The results for the TRC have been included as an attachment to the Outfall 103 reporting form. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714.

Ms. Christina Morgan Page 2 June 12, 2012 / certify under penalty oflaw that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the informationsubmitted. Based on my inquiryof the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best ofmy knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, Site Sequoyah ident uclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D.Booker, MOB 1F-WBN B. E. Brickhouse, BR 4A-C J. T. Carlin, OPS 4A-SQN J. A. Cross, POB 2A-SQN T.R. Markum, BR4A-C D. B. Nida, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 J.W. Proffitt, OPS 4C-SQN A. A. Ray,WT11A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1E-M

Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 June 12, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L &C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR MAY 2012 Enclosed is the May 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. On May 24, 2012 at 15:27 Eastern Daylight Time (EDT) visible oil sheen was identified on the east side of the essential raw cooling water (ERCW) intake building at SQN on the Tennessee River. The source of the oil sheen was caused by an oil drip pan overflow located at the C-B ERCW traveling water screen gear drive. The leak was immediately secured and the discharge was stopped. The estimated quantity of oil discharged is less than one gallon. Because the oil spill occurred in a low flow area and favorable meteorological conditions (light winds, no rain), the water was fairly stagnant and the sheen was easily contained and cleaned up utilizing absorbent booms and pads. TVA provided oral notification to Angela Young of the Chattanooga Environmental Field Office via voice message on May 24, 2012. Documentation of the incident and corrective actions are being performed in accordance with the site's Corrective Action Program. In a letter to Mr. Vojin Janjic dated December 21, 2011 SQN notified TDEC of upcoming activities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle 18 refueling outage scheduled to begin in October 2012. This project is complete and resulted in the discharge of approximately 700 gallons of water through the SQN low volume waste treatment process. The results for the pH and TSS collected during discharge have been incorporated into the DMR. The results for the TRC have been included as an attachment to the Outfall 103 reporting form. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714.

Ms. Christina Morgan Page 2 June 12, 2012 / certifyunderpenalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person orpersons who manage the system, or those persons directly responsible for gatheringthe information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware thatthere are significantpenalties for submitting false information, including thepossibility of fine and imprisonment forknowing violations. lin be President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ _TVA ^M^^HL^C^^RJPI^NT ^dress__P.OLBOX2pop_ aNJEEROFRCEOPS^N^QN) SOTD^-JDAlSY1jrN_J7384 FacHity__ _TVA_- SEQUOY^NUCLEAR PLANJ Location. Jl^lUPiLQQUNJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 05 01 12 05 31 MAJOR (SUBR01) F - FINAL DIFFUSER DISCHARGE EFFLUENT

      • NO DISCHARGE Q

NOTE: Read Instructions before completing this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 S EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE ftttftftftftftft ftftftftft&Aft MAXIMUM 1719 Req. Mon. DAILY MAX wwwwAft&Af DAILY MX UNITS 03 MGD 62 DEG C/HR MINIMUM AVERAGE 0.019 0.1 MOAVG MAXIMUM 39.4 Req. Mon. DAILY MAX 28.3 30.5 DAILY MX 1 DAILY MX 0.034 0.1 DAILY MAX UNITS 04 DEG.C. 04 DEG.C. 04 DEG.C. 19 MG/L Form Approved. OMB No. 2040-0004 NO. EX FREQUENCY OF ANALYSIS 31/31 CONTI NUOUS 31/31 CONTI NUOUS 31/31 CONTI NUOUS 31/31 CONTI NUOUS 18/31 FIVE PER WEEK 31/31 CONTI NUOUS SAMPLE TYPE RCORDR CALCTD MODELD CALCTD CALCTD CALCTD RCORDR RCORDR GRAB CALCTD CALCTD CALCTD NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify underpenaltyoflawthatthisdocumentand allattachments werepreparedundermy direction orsupervision inaccordancewith a systemdesignedto assure thatqualified personnel properly gatherand evaluatethe information submitted. Basedon myinquiry ofthe personor personswhomanagethe system,or those personsdirectly responsible forgathering the information, the information submitted is, tothe bestofmyknowledge andbelief, true,accurate, and complete. Iamawarethatthereare significant penaltiesforsubmitting false information, includingthe possibility offine and imprisonmentforknowing violations. TELEPHONE DATE John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference all attachments here) No closed mode operation. Veliger monitoring data is included as an attachment. B/CTP chemical injections during this period included 1. Floguard MS6236 (max calc cone was 0032mg/L~limit 0.2mg/L) 2. Biodetergent 73551 (max. calc. cone, was 0.032mg/L-limit 2.0mg/L) 3. Spectrus CT1300 (max. calc. cone, was 0.041 mg/L~limit 0.050mg/L). EPAForm 3320-1 (REV3/99) Previous editions may beused ~

OF PRINCIPAL EXECUTIVE FICER OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER 12 06 YEAR MO Page 1 of 1 12 DAY

Sample Date Mean# of ZM/m3 % Settlers Water Temp. (°C) Sample Date Mean# of Asiatic Clams/m3 Water Temp. (°C) NOTES: % LOCATION SUB LOCATION Gravid Asiatic Clam COLLECTED BY 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 PB 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 PB 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May 2012 No Samples Collected

PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different) Name __ _'A ^M^^HJ^^^F^l^NT ^(fes^jp.o^BOXigog. O.NIERQFjFlCEOPSJ.N^QN) SODDX-DAISYJ1N_37384 FacjHty__ _TV^-SEQUOYM IWO^^ PLANJ J^c^on_Jl>MiUPiLQQyiOY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 101 T PERMIT NUMBER DISCHARGE NUMBER YEAR MONITORING PERIOD MO DAY To YEAR MO DAY 12 05 01 12 05 31 MAJOR (SUBR01) F - FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT

      • NO DISCHARGE Q

NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 EFFLUENT GROSS IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM

wxkwAAiMIe wt&w&&1iitwt UNITS MINIMUM >100.0 43.2 MINIMUM >100.0 43.2 MIM1NUM AVERAGE MAXIMUM &4r4r A4f4r UNITS 23 PERCENT 23 PERCENT Form Approved. OMB No. 2040-0004 NO. EX FREQUENCY OF ANALYSIS 1/180 SEMI ANNUAL 1/180 SEMI ANNUAL SAMPLE TYPE COMPOS COMPOS COMPOS COMPOS NAMEHTTLE PRINCIPAL EXECUTIVE OFFICER ICertify under penallyof lawthatthis documentand allattachmentswere preparedundermy direction orsupervisioninaccordancewitha system designedto assure that qualified person' properly gatherandevaluatethe information submitted. Basedon my inquiry of the personor personswho managethe system, orthose personsdirectly responsible forgathering the information, the information submitted is, to the best of myknowledge andbelief,true,accurate, andcomplete. Iam awarethattherearesignificant penalties forsubmitting falseinformation, includingthe possibilityof fine and imprisonmentforknowingviolations. TELEPHONE DATE John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference allattachments here) Toxicity sampling was performed May 6-11. The report is included as an attachment. EPA Form 3320-1 (REV 3/99) Previous editions may be used Vice President E OF PRINCIPAL EXECUTIVE OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER 12 06 YEAR MO Page 1 of 1 12 DAY

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 103 G PERMIT NUMBER DISCHARGE NUMBER MAJOR (SUBR01) F - FINAL LOW VOL. WASTE TREATMENT POND EFFLUENT Form Approved. OMB No. 2040-0004 PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name __ _'A^EQI^^^NJK^E^R^LANT ^(fess__P.a_BOX2000. aNTERQFFICEOPSir^SQN) SODDY.-DAlSYJLTN_J7384 Facjlity_ jn^-^QUOY^NUCLEAR PLANJ Ucatjpn_ Jd/MiyPILQQLINJY ATTN: Brad Love PARAMETER From MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 05 01 12 05 31 NO DISCHARGE \\_] *** NOTE: Read instructions before completing this form. PH 00400 1 0 EFFLUENT GROSS SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS OIL AND GREASE 00556 1 0 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT QUANTITY OR LOADING AVERAGE 1.038 Req. Mon. MOAVG MAXIMUM 1.216 Req. Mon DAILY MX UNITS 03 MGD MINIMUM 6 MINIMUM NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certifyunder penaltyof lawthatthis documentand allattachmentswere preparedundermy direction or supervisioninaccordancewitha system designed to assure that qualifiedpersonnel properly gather and evaluate the informationsubmitted. Based on my inquiryof the person or persons who manage the system, or those persons directlyresponsible forgathering the information, the Information submitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam awarethat there are significantpenalties forsubmittingfalse information, including the possibilityof fine and imprisonment for knowing violations. John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here) Outfall 103 TRC analysis collected during discharge of Steam Generator Process Water from drilling core pilings is included as an attachment. EPA Form 3320-1 (REV 3/99) Previous editions may be used QUALITY OR CONCENTRATION AVERAGE 30 MOAVG <5 15 MOAVG WW WW ft ft "$1it Aft&ft&ftftft MAXIMUM MAXIMUM 9 100 DAILY MX <6 20 DAILY MX UNITS 12 SU 19 MG/L 19 MG/L NO. EX FREQUENCY OF ANALYSIS 15/31 THREE/ WEEK 2/31 TWICE/ MONTH 2/31 TWICE/ MONTH 31/31 SEE PERMIT Page 1 of 1 SAMPLE TYPE GRAB GRAB GRAB GRAB GRAB GRAB RCORDR RCORDR

DMR Attachment Outfall 103: TRC Sample collected during discharge of Steam Generator Project discharge of process water from drilling core pilings. Reference DMR Cover Letter. Outfall 103 Date/Time Collected TRC Analysis Date/Time Analyst 5/15/2012/11:10 0.03 mg/L 5/15/2012/11:24 MLW

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name ___'A-SEQI^^^r^CL^I^^NT Mdress__P.a_BOX2pog. aNJEROFRCEOPSlN^QN) SODE^-DAISYJW^7384 FacjHty___ JYA-SEQUOY^NyCLEAR PLANJ LocatLQD_ Jl/M'UPILCQLINJY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT Form Approved. OMB No. 2040-0004 From TN0026450 110 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD Y^AR MO DAY To YEAR MO DAY 12 05 01 12 05 31 NO DISCHARGE NOTE: Read Instructions before completing this form. XX PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS VALUE TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS VALUE TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM WWIXIXXW Req. Mon. DAILY MX DAILY MX UNITS 03 MGD 04 DEGC MINIMUM

            • £*

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify under penaltyoflawthat this documentand allattachmentswere prepared under my direction or supervisioninaccordance witha systemdesigned to assure that qualified personnel properlygather and evaluate the Information submitted.Based on myinquiryofthe person or persons whomanage the system,or those persons directly responsibleforgatheringthe information, the information submitted is, to the best of myknowledgeand belief,true, accurate, and complete. Iam aware that there are significantpenalties for submittingfalse information, includingthe possibilityoffine and imprisonmentfor knowing violations. John T. Carlin AVERAGE 0.1 MOAVG MAXIMUM REPORT DAILY MX 30.5 DAILY MX 5 DAILY MX 0.1 DAILY MX UNITS 04 DEGC 04 DEGC 04 DEGC 19 MG/L TELEPHONE FREQUENCY OF ANALYSIS CONTIN UOUS CONTIN UOUS CONTIN UOUS CONTIN UOUS Five per Week CONTIN UOUS SAMPLE TYPE CALCTD CALCTD CALCTD RCORDR CALCTD CALCTD DATE Sequoyah Site Vice President 423 843-7001 12 06 12 TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference allattachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNATURE OFFK PRINCIPAL EXECUTIVE >RAUTHORIZED AGENT AREA CODE NUMBER YEAR MO Page 1 of 1 DAY

PERMITTEE NAME/ADDRESS (Include Facility NameA.ocaVon if Different) Name __ _'A ^TO^^^r^CL^R^LANT ^d^ss____P.a.BOX2p00. aNTERQFRCEOPMri^QN) S0DDX-J^1SY1JN_37384 Facjljty__ JFW.- SEQUOY/^NUCLEAR PLANJ LgcatJon_Jiy^ ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 110 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 05 01 12 05 31 MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE XX Form Approved. OMB No. 2040-0004 NOTE: Read Instructions before completing this form. PARAMETER x-QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM

  • UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1

0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT w AAwA ft ff§t 23 PERCENT PERMIT REQUIREMENT ftftfttttt'Wt'lt 43.2 MINIMUM SEMI ANNUAL COMPOS IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 23 PERCENT PERMIT REQUIREMENT 43.2 MINIMUM SEMI ANNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEATITLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED I Certifyunder penalty of law that this document and all attachments were prepared under my direction or supervision in accordance witha system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. COMMENTS AND EXPLANATION OF ANY VIOLATIONS No Discharge this Period (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE 423 843-7001 AREA CODE NUMBER DATE 12 06 YEAR MO Page 1 of 1 12 DAY

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name __'*i?EQWW^IWCLEARPLANT Mdress_ J^OJJQX20go. aNJlEOFRCEOPSlN^QN) SOTDY_-DA1SYJ:N_37384 Facjnty__ JA^-SEQUOYM MOLEAR PLANJ J^catjpn_ ji>Miynpj^coyNTY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 118 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 05 01 12 05 31 MAJOR (SUBR01) F - FINAL WASTEWATER & STORM WATER EFFLUENT

      • NO DISCHARGE

[xx] *** NOTE: Read Instructions before completing this form Form Approved. OMB No. 2040-0004 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM OXYGEN, DISSOLVED (DO) 00300 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT 2 MINIMUM SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS SOLIDS, SETTLEABLE 00545 1 0 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT Req. Mon. MOAVG Req. Mon. DAILY MX 03 MGD NAMEHTTLE PRINCIPAL EXECUTIVE OFFICER ICertifyunder penaltyof lawthat this documentand allattachmentswere preparedunder my directionor supervision in accordance witha system designed to assure that qualifiedpersonnel properlygatherand evaluate the information submitted. Based on my inquiryof the person or persons who manage the system, orthose persons directlyresponsible for gatheringthe information, the informationsubmitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significant penalties for submitting false information, includingthe possibilityof fine and imprisonmentfor knowing violations. John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here) During this reporting period,there has been no flow from the Dredge Pond otherthan that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNATI OFFICEi AVERAGE MAXIMUM 100 DAILY MX DAILY MX resident PRINCIPAL EXECUTIVE AUTHORIZED AGENT UNITS 19 MG/L 19 MG/L 25 ML/L TELEPHONE 423 843-7001 AREA CODE NUMBER TWICE/ WEEK TWICE/ WEEK ONCE/ MONTH ONCE/ BATCH GRAB GRAB GRAB ESTIMA DATE 12 06 12 YEAR MO DAY Page 1 of 1

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: June 11, 2012

1. Facility / Discharger: Sequoyah Nuclear Plant / TVA
2. County / State: Hamilton / Tennessee
3. NPDES Permit #: TN0026450
4. Type of Facility: Nuclear-Fueled Electric Generating Plant
5. Design Flow (MGD): 1,579
6. Receiving Stream: Tennessee River (TRM 483.6)
7. 1Q10: 3,491
8. Outfall Tested: 101
9. Dates Sampled: May 06 - 11, 2012
10. Average Flow on Days Sampled (MGD): 1715.29, 1713.71, 1700.72
11. Pertinent Site Conditions: Production / operation data will be provided upon request.
12. Test Dates: May 08 - 15, 2012
13. Test Type: Short-term Chronic Definitive
14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested (%): Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100 Pimephales promelas: UV treated Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100
16. Permit Limit Endpoint (%): Outfall 101: IC25 = 43.2%
17. Test Results: Outfall 101: Pimephales promelas: IC25 > 100%

Ceriodaphnia dubia: IC25 > 100% UV treated Outfall 101: Pimephales promelas: IC25 > 100% Page 1 of 110

18. Facility

Contact:

Brad Love Phone #: (423) 843-6714

19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.
20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Donald W. Snodgrass Phone #: (256) 386-2787

22. Notes: Outfall 101 samples collected May 06 - 11, 2012, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >

100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 2 of 110

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101, Intake
2. Sample Type: Composite
3. Sample Information:

Sample ID Date (MM-DD-YY) Time (ET) Collected Date (MM-DD-YY) Time (ET) Received Arrival Temp. (°C) Initial TRC* (mg/L) Date (MM-DD-YY) Time (ET) Last Used By 101 05-06-12 0855 to 05-07-12 0755 05-07-12 1429 1.8, 1.8 <0.10 05-08-12 1130 05-09-12 1030 Intake 05-06-12 0910 to 05-07-12 0810 05-07-12 1429 1.0 <0.10 05-08-12 1130 05-09-12 1030 101 05-08-12 0655 to 05-09-12 0555 05-09-12 1300 1.6, 1.7 <0.10 05-10-12 1053 05-11-12 1030 Intake 05-08-12 0710 to 05-09-12 0610 05-09-12 1300 1.6 <0.10 05-10-12 1053 05-11-12 1030 101 05-10-12 0655 to 05-11-12 0555 05-11-12 1225 0.5, 0.6 <0.10 05-12-12 1030 05-13-12 1032 05-14-12 1030 Intake 05-10-12 0710 to 05-11-12 0610 05-11-12 1225 2.1 <0.10 05-12-12 1030 05-13-12 1032 05-14-12 1030

  • TRC = Total Residual Chlorine Samples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0oC) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Page 3 of 110

Pimephales promelas Ceriodaphnia dubia Test Organisms:

1. Source:

Aquatox, Inc. In-house Cultures

2. Age:

19.2 - 19.5 hours old < 24-hours old Test Method Summary:

1. Test Conditions:

Static, Renewal Static, Renewal

2. Test Duration:

7 days Until at least 60% of control females have 3 broods

3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
4. Number of Replicates:

4 10

5. Organisms per Replicate: 10 1
6. Test Initiation: (Date/Time)

Outfall 101 05-08-12 1130 ET 05-08-12 0935 ET UV Treated Outfall 101 05-08-12 1112 ET

7. Test Termination: (Date/Time)

Outfall 101 05-15-12 1030 ET 05-15-12 0842 ET UV Treated Outfall 101 05-15-12 1015 ET

8. Test Temperature: Outfall 101: Mean = 24.8oC Mean = 25.0oC (24.5 - 25.1oC)

(24.7 - 25.3oC) Test Temperature: UV-Treated Outfall 101: Mean = 24.8oC (24.3 - 25.2oC)

9. Physical / Chemical Measurements:

Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA). Page 4 of 110

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted May 08 - 15, 2012 using effluent from Outfall 101. Test Solutions (% Effluent) Percent Surviving (time interval used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 98 98 98 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions (% Effluent) Mean Dry Weight (mg) (replicate number) 1 2 3 4 Mean Control 0.658 0.686 0.707 0.778 0.707 10.8% 0.624 0.648 0.699 0.647 0.655 21.6% 0.629 0.717 0.594 0.716 0.664 43.2% 0.678 0.674 0.642 0.674 0.667 86.4% 0.660 0.604 0.657 0.648 0.642 100.0% 0.621 0.634 0.586 0.682 0.631 Intake 0.734 0.634 0.693 0.716 0.694 IC25 Value: > 100% Permit Limit: 43.2% 95% Confidence Limits: Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc* Permit Limit: 2.3 TUc

  • TUa = 100/LC50: TUc = 100/ IC25 Page 5 of 110

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted May 08 - 15, 2012 using effluent from Outfall 101. Test Solutions (% Effluent) Percent Surviving (time interval used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions (% Effluent) Reproduction (#young/female/7 days) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 33 32 30 31 36 30 33 34 31 29 31.9 10.8% 33 33 30 33 31 32 28 35 35 32 32.2 21.6% 36 32 36 33 31 32 35 32 35 33 33.5 43.2% 35 31 33 39 33 30 35 37 33 33 33.9 86.4% 34 36 32 35 37 35 34 34 33 35 34.5 100.0% 37 36 35 35 37 38 33 36 35 36 35.8 IC25 Value: > 100% Permit Limit: 43.2% 95% Confidence Limits: Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc* Permit Limit: 2.3 TUc

  • TUa = 100/LC50: TUc = 100/ IC25 Page 6 of 110

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted May 08 - 15, 2012 using water from Intake Test Solutions (% Effluent) Percent Surviving (time interval used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions (% Effluent) Reproduction (#young/female/7 days) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 33 33 30 31 29 32 31 31 26 30.6 Intake 33 35 29 32 31 36 32 31 33 31 32.3 IC25 Value: > 100% Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A

  • TUa = 100/LC50: TUc = 100/ IC25 Page 7 of 110

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted May 08 - 15, 2012 using effluent from UV Treated Outfall 101. Test Solutions (% Effluent) Percent Surviving (time interval used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 98 98 98 98 98 Test Solutions (% Effluent) Mean Dry Weight (mg) (replicate number) 1 2 3 4 Mean Control 0.591 0.647 0.507 0.599 0.586 10.8% 0.646 0.621 0.616 0.698 0.645 21.6% 0.553 0.622 0.659 0.663 0.624 43.2% 0.565 0.609 0.634 0.606 0.604 86.4% 0.537 0.573 0.610 0.560 0.570 100.0% 0.673 0.680 0.676 0.653 0.671 Intake 0.597 0.592 0.561 0.704 0.614 IC25 Value: > 100% 95% Confidence Limits: Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc*

  • TUa = 100/LC50: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC25) Pimephales promelas May 08 - 15, 2012 1236 7 days KCl 0.77 g/L Ceriodaphnia dubia May 08 - 15, 2012 0908 7 days NaCl 1.06 g/L Page 8 of 110

24.8 - 24.9 24.6 - 25.0 7.4 7.8 6.4 8.0 7.85 - 8.06 7.49 - 7.90 294 - 327 62 63 87 89 24.8 - 25.0 24.5 - 24.9 7.6 8.0 6.4 7.9 7.89 - 8.10 7.49 - 7.86 283 - 306 24.8 - 25.0 24.5 - 24.9 7.6 8.0 6.5 7.9 7.89 - 8.10 7.49 - 7.86 276 - 291 24.8 - 25.0 24.6 - 25.1 7.6 8.0 6.5 8.0 7.86 - 8.11 7.47 - 7.90 253 - 265 24.8 - 25.0 24.6 - 25.0 7.6 8.0 6.5 8.0 7.81 - 8.12 7.46 - 7.90 210 - 218 24.8 - 25.0 24.5 - 25.0 7.6 8.0 6.2 8.0 7.76 - 8.16 7.42 - 7.92 188 - 199 79 81 81 81 < 0.10 - < 0.10 24.9 - 25.1 24.7 - 25.1 7.7 8.1 6.7 8.0 7.95 - 8.16 7.53 - 7.93 182 - 195 77 79 79 81 < 0.10 - < 0.10 24.7 - 25.0 24.9 - 25.2 7.4 7.8 7.6 8.0 7.85 - 8.06 7.87 - 8.02 294 - 327 62 63 87 89 24.7 - 25.2 24.8 - 25.3 7.6 8.0 7.6 8.0 7.89 - 8.10 7.86 - 8.03 283 - 306 24.7 - 25.2 24.8 - 25.2 7.6 8.0 7.6 8.0 7.89 - 8.10 7.86 - 8.02 276 - 291 24.8 - 25.2 24.7 - 25.2 7.6 8.0 7.6 8.1 7.86 - 8.11 7.85 - 8.03 253 - 265 24.8 - 25.2 25.0 - 25.2 7.6 8.0 7.6 8.0 7.81 - 8.12 7.86 - 8.07 210 - 218 24.8 - 25.2 24.8 - 25.2 7.6 8.0 7.6 8.1 7.76 - 8.16 7.87 - 8.06 188 - 199 79 81 81 81 < 0.10 - < 0.10 24.8 - 25.2 24.8 - 25.2 7.7 8.1 7.8 8.1 7.95 - 8.16 7.87 - 8.03 182 - 195 77 79 79 81 < 0.10 - < 0.10 Overall temperature (oC) Pimephales promelas Ceriodaphnia dubia 25.0 24.7 25.3 Average Minimum Maximum 24.8 24.5 25.1 PHYSICAL/CHEMICAL

SUMMARY

performed May 08-15, 2012. Test Sample ID Temperature (oC) Dissolved Oxygen (mg/L) pH (S.U.) Alkalinity Hardness Water Chemistry Mean Values and Ranges for Pimephales promelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfall 101 Initial Final Initial Final Initial Final 24.9 24.7 7.1 7.8 24.7 7.9 24.7 7.9 (µmhos/cm) 310 88 7.98 7.97 293 283 43.2% 24.9 24.7 7.72 7.68 7.2 21.6% 24.9 24.7 7.71 86.4% 24.9 63 7.96 7.8 7.8 7.6 7.96 7.2 260 Total Residual Chlorine (mg/L) Conductance (mg/L CaCO3) (mg/L CaCO3) Pimephales promelas Control 24.8 24.7 10.8% 7.68 7.2 < 0.10 80 81 195 7.2 7.95 7.72 213 7.73 7.2 7.94 Intake 7.6 7.8 100.0% 25.0 10.8% 24.9 25.0 43.2% 25.0 190 25.0 24.8 7.9 7.3 8.06 7.75 Ceriodaphnia dubia Control 24.8 25.0 Intake 21.6% 86.4% 24.9 25.0 24.9 7.8 7.8 7.97 7.95 293 7.95 310 7.96 63 88 78 80 < 0.10 24.9 25.0 7.8 7.8 7.98 260 283 7.9 25.0 7.8 7.8 7.96 7.95 7.95 7.8 7.8 7.95 80 7.94 7.98 213 100.0% 25.0 25.0 7.9 7.98 78 8.00 < 0.10 < 0.10 195 25.0 81 8.06 7.9 7.9 190 80 Page 9 of 110

24.7 - 24.9 24.5 - 24.8 7.6 8.0 6.5 8.0 7.88 - 8.07 7.51 - 7.91 300 - 316 62 64 87 89 24.8 - 25.0 24.4 - 24.9 7.6 8.0 6.6 7.9 7.88 - 8.09 7.50 - 7.85 283 - 305 24.8 - 25.0 24.5 - 24.9 7.6 8.0 6.5 8.0 7.89 - 8.10 7.50 - 7.90 269 - 291 24.8 - 25.1 24.5 - 24.8 7.6 8.0 6.5 7.9 7.86 - 8.11 7.50 - 7.89 248 - 266 24.8 - 25.1 24.6 - 24.9 7.5 8.0 6.5 7.9 7.79 - 8.13 7.50 - 7.90 207 - 221 24.8 - 25.2 24.3 - 24.8 7.6 8.1 6.5 7.9 7.76 - 8.15 7.50 - 7.91 189 - 201 79 79 81 81 < 0.10 - < 0.10 24.8 - 25.2 24.5 - 24.9 7.6 8.2 6.6 8.0 7.94 - 8.16 7.52 - 7.95 184 - 196 77 79 79 81 < 0.10 - < 0.10 Overall temperature (oC) Pimephales promelas Intake 100.0% 25.0 192 25.0 24.7 8.0 24.6 7.9 7.74 216 7.74 7.2 78 80 79 81 < 0.10 260 7.71 7.71 7.2 8.02 7.77 198 7.2 7.94 7.94 < 0.10 Total Residual Chlorine (mg/L) Conductance (mg/L CaCO3) (mg/L CaCO3) 63 (µmhos/cm) 307 88 7.9 7.96 7.2 Pimephales promelas Control 24.8 24.7 10.8% 86.4% 24.9 43.2% 24.9 24.6 7.72 7.70 7.3 21.6% 24.9 24.6 7.97 7.97 7.97 296 283 24.9 24.7 7.2 7.9 24.7 7.9 7.2 7.9 7.9 Initial Final Initial Final Initial Final PHYSICAL/CHEMICAL

SUMMARY

performed May 08-15, 2012. Test Sample ID Temperature (oC) Dissolved Oxygen (mg/L) pH (S.U.) Alkalinity Hardness Water Chemistry Mean Values and Ranges for Pimephales promelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 101 Average Minimum Maximum 24.8 24.3 25.2 Page 10 of 110

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected May 06 - 11, 2012, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Page 11 of 110

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None
2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
1. Pimephales promelas None
2. Ceriodaphnia dubia None Page 12 of 110

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
3. Temperature was measured by SM 2550 B.
4. Dissolved oxygen was measured by SM 4500 O G.
5. The pH was measured by SM 4500 H+ B.
6. Conductance was measured by SM 2510 B.
7. Alkalinity was measured by SM 2320 B.
8. Total hardness was measured by SM 2340 C.
9. Total residual chlorine was measured by ORION Electrode Method 97-70.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCl.
2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.
4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 13 of 110

REFERENCES

1. NPDES Permit No. TN0026450.
2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
3. Standard Methods for the Examination of Water and Wastewater, 21st Edition, 2005.
4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 14 of 110

Sequoyah Nuclear Plant Biomonitoring May 08 - 15, 2012 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 110

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 11, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130M mg/L Quat 03/12/1998 0.016 03/13/1998 0.015 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.013 03/17/1998 0.020 03/18/1998 0.018 09/08/1998 0.015 0.014 0.005 0.021 09/09/1998 0.003 0.031 0.011 09/10/1998 0.014 0.060 0.021 09/11/1998 0.013 0.055 0.019 09/12/1998 < 0.001 0.044 0.015 09/13/1998 < 0.001 0.044 0.015 09/14/1998 0.008 0.044 0.015 02/22/1999 < 0.001 02/23/1999 0.005 02/24/1999 0.009 02/25/1999 0.012 02/26/1999 0.008 02/27/1999 < 0.001 02/28/1999 < 0.001 08/18/1999 0.015 0.069 0.024 0.006 08/19/1999 0.012 0.068 0.024 08/20/1999 0.023 0.070 0.024 0.120 08/21/1999 0.022 0.068 0.024 08/22/1999 0.022 0.068 0.024 08/23/1999 0.025 0.068 0.024 0.006 08/24/1999 0.016 0.067 0.023 0.020 Page 16 of 110

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 11, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130M mg/L Quat 01/31/2000 < 0.002 0.026 0.009 02/01/2000 0.011 0.026 0.028 02/02/2000 0.028 0.026 0.009 0.006 02/03/2000 0.008 0.027 0.009 02/04/2000 0.006 0.027 0.009 0.005 0.109 02/05/2000 < 0.002 0.027 0.009 02/06/2000 < 0.002 0.027 0.009 07/26/2000 < 0.0057 0.055 0.019 07/27/2000 0.019 0.055 0.019 07/28/2000 0.0088 0.053 0.018 0.004 0.108 07/29/2000 < 0.0088 0.055 0.019 07/30/2000 < 0.0076 0.055 0.019 07/31/2000 < 0.0152 0.055 0.019 0.006 08/01/2000 < 0.0141 0.055 0.019 0.005 12/11/2000 0.0143 0.025 0.020 0.005 12/12/2000 0.0092 0.025 0.020 0.005 12/13/2000 < 0.0120 0.025 0.020 12/14/2000 < 0.0087 0.025 0.020 12/15/2000 0.0120 0.025 0.020 0.005 12/16/2000 < 0.0036 0.025 0.020 12/17/2000 < 0.0036 0.025 0.020 08/26/2001 0.017 0.06 0.021 0.006 08/27/2001 <0.0096 0.06 0.021 0.005 0.021 08/28/2001 <0.0085 0.06 0.021 08/29/2001 <0.0094 0.059 0.020 0.005 0.021 08/30/2001 <0.0123 0.06 0.021 0.005 08/31/2001 <0.005 0.059 0.020 11/25/2001 <0.0044 11/26/2001 <0.0119 0.024 0.02 0.005 11/27/2001 0.0137 0.023 0.019 0.007 11/28/2001 <0.0089 0.022 0.019 0.006 11/29/2001 0.0132 0.024 0.02 0.007 11/30/2001 < 0.0043 0.024 0.02 12/09/2001 <0.0042 12/10/2001 <0.0042 12/11/2001 <0.0104 12/12/2001 0.0128 0.024 0.02 0.008 12/13/2001 <0.0088 0.024 0.02 12/14/2001 0.0134 0.024 0.02 0.007 Page 17 of 110

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 11, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130M mg/L Quat 01/02/2002 < 0.0079 0.023 0.02 0.006 01/03/2002 01/04/2002 01/05/2002 01/06/2002 01/07/2002 < 0.0042 0.0124 < 0.0042 < 0.0042 < 0.0089 0.023 0.024 0.024 0.014 0.014 0.014 0.009 0.006 02/24/2002 02/25/2002 02/26/2002 02/27/2002 02/28/2002 03/01/2002 < 0.004 < 0.004 0.0143 < 0.0041 < 0.0041 < 0.0041 0.023 0.023 0.023 0.024 0.024 0.023 0.023 0.023 0.008 0.008 0.007 05/05/2002 05/06/2002 05/07/2002 05/08/2002 05/09/2002 05/10/2002 0.058 0.058 0.056 0.057 0.056 0.02 0.02 0.019 0.02 0.019 0.014 0.015 0.014 08/04/2002 08/05/2002 08/06/2002 08/07/2002 08/08/2002 08/09/2002 <0.0058 <0.0058 0.0092 <0.0107 <0.0061 0.0152 0.053 0.053 0.055 0.055 0.054 0.018 0.018 0.019 0.019 0.018 0.007 0.008 0.025 10/06/2002 10/07/2002 10/08/2002 10/09/2002 10/10/2002 10/11/2002 <0.00497 0.0153 <0.0092 0.0124 0.0134 <0.0042 0.054 0.054 0.053 0.054 0.054 0.018 0.018 0.018 0.018 0.018 0.009 0.007 0.009 0.009 01/12/2003 01/13/2003 01/14/2003 01/15/2003 01/16/2003 01/17/2003 <0.0035 <0.006 <0.0118 <0.0063 <0.0034 <0.0034 0.025 0.026 0.026 0.026 0.026 0.019 0.020 0.020 0.020 0.009 0.009 0.009 04/06/2003 04/07/2003 04/08/2003 04/09/2003 04/10/2003 04/11/2003 <0.0073 <0.0189 <0.0117 <0.0139 <0.0113 <0.0073 0.021 0.021 0.021 0.021 0.022 0.016 0.018 Page 18 of 110

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 11, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130M mg/L Quat 06/15/2003 < 0.0045 06/16/2003 06/17/2003 06/18/2003 06/19/2003 06/20/2003 < 0.0037 < 0.0048 < 0.0048 < 0.0085 < 0.0048 0.057 0.041 0.041 0.058 0.058 0.020 0.014 0.014 0.020 0.020 0.022 0.024 0.024 0.025 0.025 08/03/2003 08/04/2003 08/05/2003 08/06/2003 08/07/2003 08/08/2003 <0.0050 <0.0050 <0.0051 <0.0084 0.0129 0.0153 0.058 0.057 0.057 0.057 0.057 0.020 0.020 0.020 0.020 0.020 0.009 0.025 0.025 0.024 10/05/2003 10/06/2003 10/07/2003 10/08/2003 10/09/2003 10/10/2003 <0.0043 <0.0043 <0.0090 <0.0106 0.0181 0.0183 0.057 0.057 0.057 0.057 0.026 0.026 0.020 0.020 0.020 0.020 0.022 0.024 0.009 0.025 0.025 0.025 0.025 02/01/2004 02/02/2004 02/03/2004 02/04/2004 02/05/2004 02/06/2004 0.0093 <0.0034 <0.0034 0.0124 <0.0034 0.0105 0.027 0.026 0.026 0.026 0.026 0.026 0.009 0.009 0.009 0.009 0.009 0.009 0.009 0.010 05/04/2004 05/05/2004 05/06/2004 05/07/2004 05/08/2004 05/09/2004 <0.0123 <0.0144 <0.0146 0.0227 0.016 <0.0104 0.026 0.026 0.037 0.058 0.060 0.058 0.019 0.014 0.013 0.020 0.021 0.020 0.009 0.009 0.025 0.025 0.025 0.025 07/04/2004 07/05/2004 07/06/2004 07/07/2004 07/08/2004 07/09/2004 0.0217 <0.0085 <0.0077 0.0252 0.0223 0.0182 0.057 0.057 0.058 0.056 0.057 0.057 0.019 0.020 0.020 0.019 0.019 0.020 0.009 0.009 0.009 0.031 0.031 Page 19 of 110

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 11, 2012 Date Sodium Hypochlorit e mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130M mg/L Quat Nalco 73551 mg/L EO/PO H-150M mg/L Quat 11/07/2004 11/08/2004 11/09/2004 11/10/2004 11/11/2004 11/12/2004 <0.0187 <0.0192 <0.0233 <0.0149 <0.0149 <0.0253 0.000 0.047 0.048 0.047 0.049 0.048 0.014 0.030 0.016 0.016 0.017 0.017 0.041 0.041 0.043 0.042 02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/11/2005 <0.0042 <0.0116 <0.0080 0.0199 <0.0042 0.0155 0.028 0.028 0.028 0.028 0.028 0.028 0.010 0.010 0.010 0.010 0.010 0.010 0.007 0.007 06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10/2005 0.0063 0.0043 0.0103 0.0295 0.0129 0.0184 0.037 0.037 0.037 07/17/2005 07/18/2005 07/19/2005 07/20/2005 07/21/2005 07/22/2005 0.0109 0.0150 0.0163 0.0209 0.0242 0.0238 0.026 0.026 0.026 0.026 0.026 0.054 0.009 0.009 0.009 0.009 0.009 0.018 0.014 0.014 0.036 0.036 0.036 10/30/2005 10/31/2005 11/01/2005 11/02/2005 11/03/2005 11/04/2005 0.0068 0.0112 0.0104 0.0104 0.0117 0.0165 0.035 0.036 0.036 0.035 11/14/2005 11/15/2005 11/16/2005 11/17/2005 11/18/2005 11/19/2005 0.0274 0.0256 0.0234 0.0231 0.0200 0.0116 Page 20 of 110

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 11, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130M mg/L Quat Nalco 73551 mg/L EO/PO H-150M mg/L Quat MSW 101 mg/L Phosphate 11/12/2006 11/13/2006 11/14/2006 11/15/2006 11/16/2006 11/17/2006 0.0055 0.0068 0.0143 0.0068 0.0267 0.0222 0.037 0.037 0.037 0.037 11/26/2006 11/27/2006 11/28/2006 11/29/2006 11/30/2006 12/01/2006 0.0188 0.0138 0.0120 0.0288 0.0376 0.0187 05/28/07 05/29/07 05/30/07 05/31/07 06/01/07 06/02/07 0.0084 0.0103 0.0164 0.0305 0.017 0.017 0.036 0.036 0.036 0.036 0.015 0.015 0.015 0.015 0.015 0.015 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 0.0241 0.0128 0.0238 0.0158 0.0162 0.0175 04/13/08 04/14/08 04/15/08 04/16/08 04/17/08 04/18/08 0.0039 0.0124 0.0229 0.0143 0.0120 0.0149 10/26/08 10/27/08 10/28/08 10/29/08 10/30/08 10/31/08 0.0260 0.0151 0.0172 0.0154 0.0086 0.017 0.018 0.041 0.041 0.041 0.041 0.030 0.030 0.030 Page 21 of 110

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test

Sampling, March 12, 1998 - May 11, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosph ate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat

-PF mg/L Azole H-130M mg/L Quat Nalco 73551 mg/L EO/PO Spectrus CT1300 mg/L Quat H-150M mg/L Quat MSW 101 mg/L Phosphate 02/08/09 02/09/09 02/10/09 02/11/09 02/12/09 02/13/09 0.0197 0.0237 0.0104 0.0155 0.0106 0.017 0.017 0.021 0.017 0.017 05/10/09 05/11/09 05/12/09 05/13/09 05/14/09 05/15/09 0.0129 0.0415 0.0053 0.0049 <0.0141 <0.0160 0.0446 0.0396 0.0396 0.0397 11/15/09 11/16/09 11/17/09 11/18/09 11/19/09 11/20/09 0.025 0.0152 0.0255 0.0306 0.0204 0.0093 05/09/10 05/10/10 05/11/10 05/12/10 05/13/10 05/14/10 0.0192 0.0055 0.0100 0.0171 0.0041 0.0099 0.039 0.039 0.039 0.039 Page 22 of 110

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test

Sampling, March 12, 1998 - May 11, 2012 Date Sodium Hypochlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosph ate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat

-PF mg/L Azole H-130M mg/L Quat Nalco 73551 mg/L EO/PO Spectrus CT1300 mg/L Quat H-150M mg/L Quat MSW 101 mg/L Phosphate 10/31/10 11/01/10 11/02/10 11/03/10 11/04/10 11/05/10 0.0122 0.0112 0.0163 0.0107 0.0132 05/01/2011 05/02/2011 05/03/2011 05/04/2011 05/05/2011 05/06/2011 0.0155 0.0179 0.0089 0.04 0.04 0.04 0.04 11/06/2011 11/07/2011 11/08/2011 11/09/2011 11/10/2011 11/11/2011 0.0168 0.0225 0.0141 0.0239 0.0242 0.0231 05/06/2012 05/07/2012 05/08/2012 05/09/2012 05/10/2012 05/11/2012 0.0145 0.0298 0.0174 0.041 0.041 0.041 Page 23 of 110

Sequoyah Nuclear Plant Biomonitoring May 08 - 15, 2012 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 24 of 110

Page 25 of 110 BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of_1_ Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client 0 Urier) P,O, Number: N/A Asheville, NC Other (specify): 28801

JraIComments
A......i"ul r'~ ~I fJjSS, Facility Sampled: Sequoyah NP

~ ~,r/...4. O1JCl o.-L ' NPDES Number: TNOO26450 Phone: 828-350-9364 S. ~ i3 JiJ n ~/Je,c} J,.;II~ rt-Z3. Collected By: 'lNs/-:" 8,'~<- tb,~ } Fax: 828-350-9368 Lynk.ll frI;//'~ P """t...1- ~ i:~~~}'8~ Container ~~. c~. Ficldldentification I Numbt:r& Flow ~ Grab/Comp. Collection Dateffime Rain Event? . Laboratp~; lIse.~ Sample Description Volume (MGD) (Mark os Appropriate) ~::".... ~, ~ '=0 Date Time Yes

IfVcs, No Trace

~mLog Airival Temp. ' By_,- Time

" Appe:ar-I\\j ~

~ : Inches .' :'Number. '. ("0'- . ~'(' , ance ~lrOIg ...... w-~~ X I~.cil

'~ '~Jt

~: l-tu\\ i-- SQN-IOI-TOX Comp ~S....2(2.5gal) "~ '~.. ,?,'~t:=: :](2.5 gal) JJ;fl ii¢iH:02. ' t.. (-t1.'\\ .~ SQN-INT-TOX Comp I.. O-C* -~,.,:,'.' Sample Custody - Fill In From Top Down 1- (.\\lS~~ <;'U'l1S '....Tl\\tt. Si\\,",'I.4!S ~cel Dale/Time Received By (Signature): IN"".. ~""..I<l. _\\ "-':::, ~;~..I'\\u. Relinquished By (Signature): Dateffime ~IJ~ a. IlJ t. ~-O~ J-~ 7-/2. /0)0 E.~ ;f?, f( 52 j.,' (J..,_ Sol'o)\\(.. '-/- ',,,-.* J f) --"~f.,,/ O:r'o? _ J ;;), / lJ:,17:> £.1" $.1<,. ~/ ~\\c.... 0':.' (/"\\.l'\\... \\<.G E'\\ (\\Ut(Iv--' E'TS \\'-\\7..,\\ O':>-<r\\- 11... e,- ~f Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in icc. The hold time for each sample is 36 hours from the time of collection. Therefore; please collect and ship in such a way that the laboratory will receive the samples willI ample time to initiate testing within that time frame. Samples shipped O'vcmight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Page 26 of 110 @~iETS ~.. ~5o/ullo....lnc. 'filJ£_ll Whole Emuent Toxicity Sample Receipt Log Page -rz.-

  • Sample temperature perfOl:med using Samp\\eReceiving Thermometer: SN 633.8.

nat< Time Received Received Sample Project Sample Sample DBIDe and desCription State Comments ~""'" by 'rom temperature ('C)" number OS..Q7~12 142' J. Sumner TVA Courier 1.8/1.8 787S 120507.01 TVA-S " Nuelear Plant - Outfall 101 1N 05-07-12 1429 J. Sumner TVA Courier 1.0 7875 120507.02 TVA-S Nuclear Plant - Intake 1N OS-Q7-12 1451 J. Sumner TVA Courier 1.7 7876 120507.03 TVA - Watts BarNuclear Plant - Outfall101 1N 05-07-12 1451 J. Sumner TVA Courier 3.7 7876 120507.04 TVA - Watts BarNuclearPiant-Intake 101 1N 05-07-12 1451 J. Sumner .TVA Courier 2.' 7816 120507.05 TVA -Watts BarNuclear Plant - Outfall 113 1N OS-oH2 1451 J. Sumner TVA Courier 3.5 7876 120507.06 TVA - Watts Bar Nuclear Plant* Intake 113 1N SOP G4 - Exhibit 04.2, revision 01-03*12

Page 27 of 110 ~ BIOMONITORING CHAIN OF CUSTODY RECORD Page __l __ of__l__ Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyab NP Toxicity 351 Depot Street. FedEx ups Bus Client ~ P,O, Number: N/A Asheville, NC Other (specify): ().r:,:;S Facility Sampled: Sequoyab NP 28801 GeneraIComments:~;~....f 0"W:-/1..-r-b'6~:;' Phone: 828-350-9364 ,..,..I.. -I-iM-..k<- -+ ~:zs: NPDES Number: TNOO26450 Collected By: Dustin Bi~n il~ ~" Fax: 828-350-9368 L'ftlck.J1 ,} ~M.s ' Container

i;~~~l::;ii1berut~~'U::*"~~:'~).*'*:::' ';:, " ',.

Field Identification I Number & Flow Rain Event? Sample Description Grab/Comp, Collection DateITirne Volume (MGD) Collected (Mark as Appropriate) Dale Time Yes IfYC3. No Trace , &-510& }Jii,,81~Temp. \\, B\\j 'Time~ '..,Appear-.

  • NU01~;:*~~'

-:~ E."f Inches c' (*0),: "alice ~ ~_ 't'". ~;:- I:-+',"SS ~/S.?, r/'-E: 1~9.;lS' :t:~;c. h:~~ ;r.;. ,~ SQN*IOI-TOX Comp o n f.,. 0$55. r" 2(2.5gal) ..".. /' "fiu,ci: X \\.~OO >1:. fj'*',,,,*:-.... SQN-INT-TOX Comp 'J/D~Z' \\--.-t 0"1-/6 ~I(2_5 gal) IJ/,q X lib~%I, r'i¥'.. :".:M- ',,;~' -~ -11:- DS/~}l. ,..". 0../0 '" **.lD C-

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  • Sample Custody - Fill In From Top Down

~ ~t>" ~l.S 1'"""-.", ~Wl.e!; ~c.ellieb I" """.. (J)..u\\..o~. ~,...._ IN Relinquished By (Signature): DatelTime Received By (Signature): DaleITime d' _.LJ \\VA $ON\\C tJ~tJ'f.. J71 /lui;;. f'Y-.n, I J-/df)- fj f{, ~~O _"-:-::-"7-;0 .? O'Y>/5 e-t" t1li"- I'::'-~ ~R 2..4-P'-J .::o~C <J s.... D"'1- / '- 'la tJJE.-r \\Iv------ c'S 0$-6'\\-1<_ \\~C £i '-.../ Instructions: Clients should fiU in all areas except those in the "Laboralory Use" blod(. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore. please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or the)' will not arrive until the following Monday. 'I'

Page 28 of 110 ldH I~~} iETS -6 EnvI_1T_So/utIo.... _ Whole Effluent ToxicitySample Receipt Log* page~ "'Sample temperature performed using Sample Receiving Thermometer: SN 6338. Date TIme Reteived Received Sample Project Sample Sample Dame lind description State Comments received ....w.d bv from temperature ('C)" number OS.()9-12 1002 KKomm Fed-Ex 1A 7898 120509.01 CORRERI~AAFM " Inmrnational NC OS.()9-12 1002 KKomm Fed-Ex 2.0 7899 120509.02 Bladenboro WWTP NC 05-09*12 1002 KKomm Fed-Ex 1.7 7900 120509.03 Duke En - Belews Creek Steam Station NC 05-09-12 1002 KKomm Fed-Ex 1.5 7901 120509.04 Pro essEn -C eFearS.E. NC 05-09-12 1002 K.Keenan Fed -Ex 1.5 7901 120509.05 Pro ,Enu e Fear S.E. - U "1,, NC 05-09-12 1002 KK~ Fed-Ex 0.7 7902 120509.06 Ci ofGastonia - Dallas WWTP NC 05-09-12 1002 K.Keenan Fed-Ex 5.' 7903 120509.07 United Water-Enfield WWTP NC OS..()9-12 1002 KKomm Fed-Ex 2.3 7904 120509.08 Duke En - Marshall Steam Station NC 05-09-12 1002 KKomm Fed-Ex I., 7905 120509.09 Ammons Resources - Ralei CC NC 05..09-12 1002 K.Keenan Fed,~Ex 2.6 7906 120509.10 B,O rises - Scarlett Acres MHP NC Os..o9-12 1002 KKomm Fed -Ex 5.' 7907 120509.11 United Water - Scotland Neck WWlP NC 05-09-12 1002 KKomm Fed -Ex 1.7 790. 120509.12 Duke En - McGuire NS - Outfall 005 NC 05-09-12 1002 KKomm Fed-Ex 2. 790' 120509.13 Duke En ~ McGuire NS - Outfall 001 NC 05-09-12 1002 K. Keenan Fed -Ex 1.7 7910 120509.14 Duke En ~ McGuire NS - Outfall 002 NC 05-09-12 1002 K. Keenan Fed* Ex 1.5 7911 120509.15 Elementis NC 05-09-12 1002 KK~ Fed-Ex 1.1 7912 120509.16 CarolinaBeach WWlP NC 05-09*12 '020 K. Keenan UPS 0.' 7913 120509.17 SouthC WWTP NC OS-09-12 1020 K.Keenan UPS 5.' 7914 120S09.18 Enviro Chemists - Manteo WWTP NC 05-09-12 1020 K.. Keenan UPS 2.0 7915 120509.19 Microbae Environmental* Rockin WWTP NC 05-09-12 1020 K.Keenan UPS 2.0 7916 120509.20 Microbae Environmental-Roseboro WWI'P NC Os..o9-12 1020 KK..... UPS 2.0 7917 120509.21 Microbae Environmental-JP Stevens* W WWTP NC 05-09-12 1020 KKomm UP, 2.0 7918 120509.22 Microbae Environmental-TransMontainge* Outfall 002 NC OS-09-12 1020 K. Keenan UPS 2.0 7918 120S09.23 Microbac Environmental-TransMontain - Outfall 003 NC 05-09-12 1125 KK~ Dash Courier 1.2 7919 120509.24 OWASA NC OS-09-12 1300 J. Sumner TVA Courier 1.611.7 787S 120S09.2S TVA-S " NuclearPlant-Outfal1101 TN OS-{)9-12 1300

1. Sumner TVA Courier 1.6 71175 120509.26 TVA-S uoyah Nuclear Plant - Intake TN 05-{)9-12 1522
1. Sumner TVA Courier 2.2 7876 120S09.27 TVA - Watts Bar Nuclear Plant - Outfall 101 TN 05-09-12 1522 J. Sumner TVA Courier I.,

7876 120509.28 TVA - Watts Bar Nuclear Plant - Intake 101 TN 05-09-12 1522 J. Sumner TVA Courier 2.5 7876 120509.29 TVA - Watts Bar Nuclear Plant - Outfall 113 TN 05-09-12 1522 J. Sumner TVA Courier I., 7876 120509.30 TVA - Watts BarNuclearPlant - Intake 113 TN SOP 04 - Exhibit 04.2, revision 01-03-12

Page 29 of 110 BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of_l_ e Client Other (specify): Delivered By (Circle One): FedEx UPS Bus General Comments:,tl-ri~.....+evtf-/f....+05S5 """"".,j.. ;nl-k- -f 0630. 28801 828-350-9364 828-350-9368 Environmental Testing Solution, Inc. 351 Depot Street. Asheville, NC Collcction Date/rime ~~2' Comp Grab/Comp. SQN*INT-TOX Field Jdentification I Sam.ple Description Facility Sampled: Sequoyah NP P.O. Number: N/A Project Name: Sequoyah NP Toxicity Client: TVA NPDES Number: TNOO26450 I Phone: Collected By: Dustin Bige~"'i'II~ fJ-.J L"n.Je)( \\.-.h ,pM.s,.::::z~i!I1l =:::;;E;;;;:,-"T""---r---""T"-----.L--,.~~~~~~~"":'""'~~::__"":'""'...___J "QlY:l):.Ql;f qe>-:)'5' 0:';';'.-1<,. T.~' 0",* (~~~) Rain Event?.

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,By.',. rime :;': :r;A'ppear* Inches ..::~~:i'_.. :. . (0 "'.-:: _.:~'.:... 'f...'V _~.. *':ance* )(.. r§:~.l;'i:o 1J~'(. {OlJ'b.~<.~~i~.:, c~i-y... \\~fI~. ';'H*(..~>.;~;, ':>~;C<*' ". Sample Custody - Fill In From Top Down ,., L~"","'-\\ ':>eA\\!o I~. ~",I'\\.eS (I~ Dateffime Relinquished By (Signature): ' oooll U>"l>'~>l. ~ Ht.!>W"l '" flu. Daterrime 61u.--- 0"-1\\- 11... \\1."tS e..- 0'8,3 0 Eo, os j/, /z ETS 50 p..l\\L Received By (Signature): f t~t<l"i-<\\ ('r/~ oH,., ~.£I tt U;,.1, '5op..l\\(.\\oS-I/-/2. , TV...., h'Y 'o$,/ZO/,2. 0, B~.2L.e" Instructions: Clients should fill in all areas except those in the "Laboratory Usc" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. 'Therefore, please collect and ship in such a way thai. the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx. or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Page 30 of 110 i;::>"" ~ ~iil iET'S 6* E......-TestlngS.....-.ln'" Whole Emuent Toxicity Sample Receipt Log Pa",,_:lJo tSampletemperature perfimned using Sample Receiving Them1ometer: SN 6338. Dale Time Ref:eived Ro"""" Sample Project Sample Sample name and description State Comments Ie_ bY from temperature ('q" number 05-11-12 1014 K K<enan Fed -Ex 0.7 7898 120511.01 CORRERI-AAFM Intemational NC 05-11*12 1014 K. Keenan Fed -Ex 1.0 7899 120511.02 Bladenboro WWTP NC 05-lH2 1014 K. Keenan Fed-Ex 1.6 7900 120511.03 DukeEnetl1Y - Belews Creek Steam Station NC OS-Il-12 1014 KK~ Fed-Ex 0.4 7901 120511.04 Pro ssEn eFear S.E. NC 05-11-12 1014 K. Keenan Fed-Ex 0.' 7901 120511.05 Pro ssEn -C e Fear S.B. - U stream/Intake NC 05-11-12 1014 Ie. Keenan Fed-Ex U 7902 120511.06 Ci ofGastonia - Dallas WWlP NC 05-11-12 1014 K. Keenan Fed-Ex 1.5 7903 120511.07 United Water* Enfield WWTP NC 05-11-12 1014 KKooo.. Fed-Ex I., 7904 120511.08 Duke En - Marshall Steam Station NC 05-11-12 1014 KKooo.. Fed-Ex 1.2 79<J5 120511.09 Ammons Resources - Ralei CC NC 05-11-12 1014 K.Keenan Fed -Ex 22 79<J6 1205lUO Bol rises - ScarlettAcres MHP NC 05-11-12 1014 K.lCeenan Fed -Ex 1.5 79<J7 120511.11 United Water* Scotland Neck WWTP NC 05-1H2 1014 K K<enan Fed-Ex 0.9 7908 12051U2 DukeEn - McGuire NS - Outfall 005 NC 05-11~12 1014 K K<enan Fed-Ex 1.0 7911 12051LJ3 Elementis NC OS-l1~12 1014 KK~ uPS 0.8 7913 120511.14 Srn>thC WWIP NC 05~1l-12 1014 K.Keenan UPS 0.8 7915 120511.15 Microbac Environmental - Rockin ~WWIP NC 05-11-12 1014 K. Keenan UPS 0.8 7916 120511.16 Microbac Environmenta1 - Roseboro WWTP NC 05~1l-12 1014 KK~ UPS 0.8 7917 120511.17 Microbac Environmental* Jp Stevens _W WWIP NC 05-11-12 1014 KKooo.. UPS 0.8 7921 I20511.18 Microbac Environmental - Motiva ses,LLC NC 05-11-12 1123 K.Keenan Dash Courier 0.9 7919 120511.l9 OWA$A NC 05-1I-12 1225 J. Sumner TVA Courier 0.510.6 7875 120511.20 TVA-S ah Nuclear Plant ~ Outfall 101 IN 05-11-12 1225 J. Sumner TVA Courier 21 7875 120511.21 TVA - S u 3h Nuclear Plant - Intake IN 05-11-12 1437 J. Sumner TVACowier 3.6 7876 120511.22 TVA-WattsBarNuclearPIant-OutfalIIOl IN 05-1H2 1437 J. Sumner TVA Courier '4.5 7876 120511.23 TVA ~ Watts Bar Nuclear Plant ~ Intake 101 IN 05-1H2 1437 J. Sumner TVA Courier 2.' 7876 120511.24 TVA - Watts Bar Nuclear Plant - Outfall 113 IN 05-1H2 1437 J. Sumner TVA Courier 5.8 7876 120511.25 TVA - Watts Bar Nuclear Plant - Intake 113 IN SOP 04 - Exhibit 04.2, revision 01-03-12

Page 31 of 110 Page 1 of6 County: Rhea Outfall: 101 I----------f-l!~~~ "'"'0 ,,\\10 I-----------+=~=-.:::..-..-I pH =, .'"'\\~ S.U. Tern erature = 1.."1.a °C Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Sequoyah Nuclear Plant NPDES #: TN0020168 Project #: -'--I-Q&f-I1L.woS.a------ Transfer bowl information: Average transfer volume: Dilution preparation information:. Comments: Dilution prep (%) 10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0 Total volume (mL) 2500 2500 2500 2500 2500 Dailyfeeding and renewal information: Average weight per surviving larvae: MHSW batch used Sample numbers used SOP AT20 - Exhibit AT20.3, revision 06-01-11 Test initiation, renewal, or termination Time Anal st Acceptance criteria Afternoon feeding Time Morning feeding Date Control in ormation: % Mortality: Avera e wei t er initial larvae: o Day

Page 32 of 110 IndOjlend,nt I\\OI'i<wiJy ."'tit". SOP AT20 - Exhibit AT20.3, revision 06-01-11 Day CONTROL 10.8% 21.6% A B C D E F G H 1 J K L 0 10 10 (0 10 10 fO 10 10 10 10 '0 ' /0. 1 /0 10 ID 10 10 10 If;) 10 10 10 /0 10 2 10 10 (0 10 10 /0 IC IC 10 10 '0 10 3 1"0 /0 10 10 to 10 /0 10 10 10 10 10 4 10 10 Ie> Ie 10 10 10 10 Itl 10 Itl 10 5 10 10 10 10 10 10 10 "~ 10 10 10 10 6 Ie to 10 10 10 10 10 ID 10 10 '\\ 10 7 Ie 10 10 10 '0 10 10 10 10 'I 10 10 A - Pan weight(mt 1'Jl", Tray color code:: W ~ \\~.,'i 'L*tS 14.*~ 13*14

14. (.,0; lo;.~4 '+'of

'3.3'1 \\Zo.91. 14.", 14-.Di,4-." Analyst: M Date: 0$.07...11. B - Pan + Lan:aew1ht (mg) 2D,Z,1-1'f.1t41-\\,1.'1 2./,'15 ~.&f I'!*ett I'U~ 2J.Tl ZO*Ol 'lJ.t:t-Analyst: {l~ 22 11,011 Date: O!F.I'L C - Larvae weight (mg) - B - A c..SS c.. ll.1o '.0,.,g '-."t-l vt& Co fI"\\ 10.'\\"\\ 10.1.'\\ 1.n 5."\\'1 ,.11. Hand calculated.........\\ f.. Analyst: Weight p~r initial number of larvae (mg) = C / Initial number of larvae ~ ~ ~ /'0.,. ~ '" &0.,'::1., Hand calCUlated. oJ ",,;F $' ,,(;- ,)" ~ ~ \\jl \\I \\0 \\II I'i Analyst: ~ O* "0' r:! ()' O' I)' 0' O* 0' O' (l' I)' Averageweigbt per Percent reduction '.'57. initial number of from control (%) 0.'01 O,c..~

o. 1010'-\\

1.0. \\i. larvae (mo) Page 2 of6 Date: Survival and Growth Data Species: Pimephales promelas Client: TVA I SeQuoyah Nuclear Plant, Outfall 101, Non-treated Comment codes: c ~ clear, d = dead, fg = fungus, k = killed, m = missing, sk ~ sick, sm = unusually small, Ig =unusually large, d&r = decanted andreturned, w =wounded. Comments: i""f¥i:;;gn:",~ I .~ =E"'-'~-"~-'"-talTo-..-trng-cso-'",-',"-",-", I I I I I I I I I I I I I I I I I

Page 33 of 110 I I I I I I, I I, I ~ ~ I, I "I Page 3 of6 Species: Pimephales promelas Client: TVA / Se9uoyah Nuclear Plant. Outfall 101. Non-treated Date: oS*at*\\J-Survival and Growth Data Day 43.2% 86.4% 100% M N 0 p Q R S T U V W X 0 10 10 10 10 10 1(;) 1(:) 10 10 10 10 (0 1 Ie 10 10 10 I() If) It) It) 10 10 10 10 2 I/;) 10 10 10 '0 10 10 10 '0 10 /CJ 10 3 10 10 10 10 It> 10 /0 10 Ie (0 10 /D 4 IQ /0 /0 (0 ID /0 10 ID 10 10 10 10 5 ID ID to /0 It) /0 10 /0 10 10 '0 10 6 10 10 10 10 10 ID 10 /0 10 10 1'0 10 7 10 10 Ie 10 /(:) I() 10 If) /0 Ie 1(:) 10 A - Pan weight (mgL Tray color code:: Itt\\. Yi... If.~o 14.t.'I 14:n Il.,4S 1'3*ft 3*l.t I!.ol \\'3.1.4-I~.t!> 14*'18 14-.t.S 1~.~8 Analyst: m Date: 0S."01A'L ~;a~~~:+ Lar~rht (mg) 121*0& 'Wf3 ll.l't 1.u. 'lD.5'Z-1'i,"H ".W Iq.ll*~ Itt.lflf 1J.!Z. 20.51 ~o.110 Date: t6.\\. C,... Larvae weight (mg) - B - A Hand calculated.,j Io.,g 10.'" ",.\\{t. Co.' 10.1,,0 Co,b'l ",.!>' "',.. ~ c...'1\\ Io*.,,~ 5.(tlo "'.~1-Analyst: Weight per initial number of larvae (mg) = C I Initial number oflarvae ~ ,,~ .:>.,q. No J- ~ .J>'l/ ~ ~O f;):>".:- Hand calculated. 01- '0 '11 \\Ii \\0 \\II ':) Analyst: O* o* tl' O' 0' O* \\:)' O' O* O' O' O' Average weight per Percent reduction o.r..bi S.1 l. O."-1'2.. '\\,'2.7*. o.\\,,'M ID. &7" initial number of from co~trol (%) larvae (mil) Comment codes: c = clear, d ~ dead, fg = fungus, k = killed, m = missing, sk ~ sick, sm = unusually small, 19 ~ unusually large, d&r ~ decanted and returned, w ~ wounded. Comments: Ind..,.ndon, ll<vIewbv R"' SOP Ar20 - Exhibit Ar20J, revision 06'01-1 1

Page 34 of 110 Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant. Out[alll0l. Non-treated Date: os.at-I'l-Page 4 of6 \\.&7. Survival and Growth Data 100% Intake y Z AA BB 10 10 10 10 Ie 10 10 10 10 ID If) 10 10 10 10 10 IQ 10 to to 10 ID ID to It) ID IC) 10 It If) 10 10 14:.\\ o'M3 13.'to 05.', 1l.SS 1'Ia 10*.,3 2O.lor ,.'!M l..i-\\ 1o.4\\~,.lb o Day Environmental TestingSolutions, Ine, ET 5 4 A = Pan weight (mgL Tray color code::

  • "0n~"l-,---,=t',wl...~-~

Analyst: Mu:t:- Date: 0,&'01- \\1< 1 B = Pan +~Larva wei t(.mg) Analyst: Date: C = Larvae weight (mg) = B - A 7 2 3 6 Hand calculated. I ~ Analyst: ~,.*-- Weight per initial number of larvae (mg) = C I Initial number of larvae Hand calculated. !!' Analyst: . cp'" Average weight per Percent reduction initial number of from control (%) larvae (m ) Comment codes: c =clear, d = dead, fg =fungus. k = killed. m =missing, sk =sick, sm = unusually small. Ig = unusually large, d&r = decanted and returned, w = wounded. Comments: ImloF"'ndonl ~=. K.l':l~: ~...n ~i: SOP AT20 - Exhibit AT20.3, revision 06-01-11

Page 35 of 110 I ~H ,"n TVA I Sequoyah Nuclear Plant, Outfall 101 Non-treated May 08-15, 2012 7875 Project number: Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification ofData Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc, Not for Com liance Assessment, Internal Laborato QC Cwn:elltratioD (%) .R~pu...te lDifilll nmber of Fin.lllumber ofllln'ae A-Pan weight (mg) B m Pan + Larvae Larvae weight (mg) Weight I SQl'\\iving Mellllwelghtl CoefliCiellt ofvariatloll Weight I IDillalllumber Mean nrvival Mean weig'!I IlIltl.ol CoefIielenf of I'eJftnt redaction from larvae welght(mg) =A_B ...mberofla""lle(mg) S.....lvlng umber of (M..n.."""......._~ ofJarvu(mg) .umber "'larvae ....ri.tioD (Mt......... e..afrol (%) larvae (mg) ...........,......)(%) (mg) .......'ol....b<r.r....ooj A 10 10 l3.69 20.27 6.58 0.658 0.658 Control B 10 10 12.78 19.64 6.86 0.686 0.707 7.2 0.686 100.0 0.707 7.2 Not applicable C 10 10 14.17 21.24 7.07 0.707 0.707 D 10 10 13.67 21.45 7.78 0.778 0.778 E 10 10 14.65 20.89 6.24 0.624 0.624 10.8% F 10 10 15.54 22.02 6.48 0.648 0.655 4.' 0.648 100.0 0.655 4.' 7.5 G 10 10 14.07 21.06 6.99 0.699 0.699 H 10 10 13.39 19.86 6.47 0.647 0.647 I 10 10 12.86 19.15 6.29 0.629 0.629 21.6% J 10 10 14.60 21.77 7.17 0.717 0.681 6.4 0.717 U K 10 9 14.08 20.02 5.94 0.660 0.594 97.5 0.664 9.4 L 10 10 14.11 21,27 7.16 0.716 0,716 M 10 10 14.30 21.08 6.78 0.678 0.678 43.2% N 10 10 14.69 21.43 6,74 0.674 0.667 2.5 0.674 5.7 0 10 10 14.77 21.19 6.42 0.642 0.642 100.0 0.667 2.5 P 10 10 12.48 19.22 6.74 0.674 0.674 10 10 13.72 20.32 6.60 0.660 0.660 86.4% R 10 10 13.67 19.71 6.04 0.604 0.642 4.0 0.604 100.0 0.642 4.0 9.2 5 10 10 13.07 19.64 6.57 0.657 0.657 T 10 10 13.24 19.72 6.48 0.648 0.648 U 10 10 13.23 19.44 6.21 0.621 0.621 100% V 10 10 14.98 21.32 6.34 0.634 0.631 6.3 0.634 10.8 W 10 10 14.65 20.51 5.86 0.586 0.586 100.0 ~6JI 6.3 X 10 10 13.58 20.40 6.82 0.682 0.682 Y 10 10 14.01 21.35 7.34 0.734 0.734 100% Intake Z 10 10 13.43 19.77 6.34 0.634 0.694 6.3 0.634 100.0 0.694 6.3 I.' AA 10 10 13.70 20.63 6.93 0.693 0.693 BB 10 10 13.51 20.67 7.16 0.716 0.716 ~ Olltfall101: Dunnett's MSD value: PMSD: 0.0697 9.9 MSD= PMSD= Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Intake: Dunnett's MSD value: PMSD: ~0653 9.2 Lower PMSD bound determined by USEPA (IOth percentile) = 12%. Upper PMSD bound determined by USEPA (90th pe£centile) = 30%. Lower and upper PMSD bounds we£e determined from the 10th and 90th percentile, respectively, of PMSD datafrom EPA's WET Interlaboratory Variability Study (USEPA, 200 la; USEPA, 200 Ib). USEPA. 200180 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-OO4 and EPA-821-B-01-OO5. US Environmental Protection Agency, Cincinnati, OR. File: sqn101_050612dala.xlsx Entered by: J. s~rer Reviewed by:~

Page 36 of 110 i~TS ,,:>.Snvl",nm.ntal r.,lIng Sal"IIO~',Inc. TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 08-15, 2012 Statistical Analyses Larval Fish Growth and Survival Test-? Day Growth TestlO: PpFRCR Sample ID: TVA! SQN, Outfall 101 Lab 10: ETS-Envir. Testing Sol. Sample Type: DMR.Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP*Pimephales promeJas Start Date: End Date: Sample Date: Comments: Conc-% 5/8/2012 5/15/2012 May 2012 Non-treated 1 2 3 4 D-Control 10.8 21.6 43.2 86.4 100 Intake 0.6580 0.6240 0.6290 0.6780 0.6600 0.6210 0.7340 0.6860 0.6480 0.7170 0.6740 0.6040 0.6340 0.6340 0.7070 0.6990 0.5940 0.6420 0.6570 0.5860 0.6930 0.7780 0.6470 0.7160 0.6740 0.6480 0.6820 0.7160 Transform: Untransformed 1-Talled Isotonic Conc*% Mean N*Mean Mean Min Max CV% N t-Stat Critical MSO Mean N*Mean D-Control 0.7073 1.0000 0.7073 0.8580 0.7780 7.248 4 0.7073 1.0000 10.8 0.6545 0.9254 0.6545 0.8240 0.6990 4.839 4 1.823 2.410 0.0697 0.6618 0.9358 21.6 0.6640 0.9388 0.6640 0.5940 0.7170 9.380 4 1.495 2.410 0.0697 0.6618 0.9358 43.2 0.6670 0.9431 0.6670 0.6420 0.6780 2.515 4 1.391 2.410 0.0697 0.6618 0.9358 86.4 0.6423 0.9081 0.6423 0.6040 0.6600 4.049 4 2.247 2.410 0.0697 0.6423 0.9081 "100 0.6308 0.8918 0.6308 0.5860 0.6820 6.298 4 2.644 2.410 0.0697 0.6308 0.8918 Intake 0.6943 0,9816 0.6943 0.6340 0.7340 6.270 4 Auxiliary Tests Statistic Critical Skew Kurt Shaplro-Wllk's Test indicates normal distribution (p> 0.01) 0.97186 0.884 0.17128 ~0.4665 Bartlett's Test indicates equal varIances (p = 0.39) 5.23389 15.0863 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 86.4 100 92.9516 1.15741 0.06973 0.09859 0.00279 0.00167 0.19306 5,18 Treatments vs D-Control " indicates IG estimate less than the lowest concentration 150 50 100 Dose % 1.0,------------, 0.9 0.8 0.7 31 0.6 cg, 0.5 &0.4 0.3 0.2 0.1 l~:=;:::'~::::::::-+:~~J 0.0 o Linear Interpolation (200 Resamples) 95% CLfExp) Skew so Point IC05" 8.409 IC10 93.170 IC15 >100 IC20 >100 IG25 >100 IC40 >100 IG50 >100 Dose-Response Plot T_~ .L 0.9,-------------------, 0.8 0.7 r---.T T ......~ J 0.6 ~ oI; 0.5 ia'0.4 Q .... 0.3 1~tall, 0.05 level of significance 0.2 0.1 0 g ~ ~ ~ ~ 0 J! 0 N M 0 ~ 5 0 ~ "cl ImF>I'nd."t Rt\\II""'b~ K'IIo~:....n File: sqnlO1_050812dala.:dsx Entered by: J. S':fer Reviewed by: ~

Page 37 of 110 Start Date: End Date: Sample Date: Comments: 5/8/2012 5/15/2012 ~6}D Non-treated TVA / Sequoyah Nuclear Plant, Intake Non-treated May 08-15, 2012 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Test ID: PpFRCR Sample ID: TVA / SON, Intake Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Conc-% 1 2 3 4 D-Control 10.8 21.6 43.2 86.4 100 Intake 0.6580 0.6240 0.6290 0.6780 0.6600 0.6210 0.7340 0.6860 0.6480 0.7170 0.6740 0.6040 0.6340 0.6340 0.7070 0.6990 0.5940 0.6420,- 0.6570 0.5860 0.6930 0.7780 0.6470 0.7160 0.6740 0.6480 0.6820 0.7160 Transform: Untransformed Conc-% Mean N-Mean Mean Min Max CV% N D-Control 0.7073 1.0000 0.7073 0.6580 0.7780 7.248 4 10.8 0.6545 0.9254 0.6545 0.6240 0.6990 4.839 4 21.6 0.6640 0.9388 0.6640 0.5940 0.7170 9.380 4 43.2 0.6670 0.9431 0.6670 0.6420 0.6780 2.515 4 86.4 0.6423 0.9081 0.6423 0.6040 0.6600 4.049 4 100 0.6308 0.8918 0.6308 0.5860 0.6820 6.298 4 Intake 0.6943 0.9816 0.6943 0.6340 0.7340 6.270 4 1-Tailed t-Stat Critical MSD 0.387 1.943 0.0653 Critical Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.01) F-Test indicates egual variances (p = 0.79) Hypothesis Test (1-tail, 0.05) Homoscedastic t Test indicates no significant differences Treatments vs D-Control Statistic 0.97462 1.38665 MSDu MSDp 0.06534 0.09238 0.749 47.4683 MSB 0.00034 Skew Kurt 0.1774 -0.6068 MSE F-Prob df 0.00226 0.71238 1,6 Dose-Response Plot 0.9 0.8 0.7 lO.6 e 0.5 C) ~0.4 C to- 0.3 0.2 0.1 0 g co CC! N 0 ~ 0 M cD 0 c: c:;; co 0 .s; () 0 1-tail, 0.05 level of significance File: sqnlOl_050812data.xlsx Entered by: J. Sumner Reviewed by:+

Page 38 of 110 Page 5 of6 SOP AT20 - Exhibit AT20.3, revision 06-01-11 Analyst 100% 10.8% 21.6% 43.2% 86.4% 100% Intake CONTROL Non-treated Species: Pimephales promelas Date: _J.l",)(=,ollL'...I",--,==-__ Client: TVA / Seguoyah Nuclear Plant. Outfall 101. Nou-treated Dail Chemist Concentration I I

Page 39 of 110 1 1t j Species: Pimeoha/es prome/as Client: TVA / Se uo ah Nuclear Plant Outfall 101 Non-treated Concen-Parameter tration CONTROL P.'~~~---I-'--'--'--- Non-treated 10.8% 21.6% 43.2% 86.4% 100% 100% Intake RoIli'WIly K'II~...n Page 6 of6 Da SOP AT20 - Exhibit AT20.3, revision 06-01-11

Page 40 of 110 7875 Project Dumber:------ Daily Chemical Analyses Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 08-15, 2012 ~ Irim "~~i ~~TS ~ Environmental Testing Solutions, Inc. Concentration IParameter 25.01 24.81 24.61 7.62 8.101 7.49 7.0 7.61 6.4 291 24.8 24.8 24.7 7.64 8.10 7.49 6.9 7.6 6.6 lI\\ll!II 281 24.8 24.8 24.7 7.63 8.11 7.47 7.0 7.6 6.5 263 25.1 24.8 24.7i 7.65 8.12 7.46 6.9 7.6 6.~ 218 24.8 8.i6 7.6 199 8.02 Da Initial Da 7.4 i941l 7..98 Initial 7.7 7.85 Final 2 Final I Initial I Final o I Davl 25.1 Total Residual Chlorine (m DO (meiL Conductivity (umbos/em' Temperature ('C) I 24.9\\ 24.6\\ 25.01 24.7 '" (Sm I 7.911 7.901 8.011 7.82 !H(SU Temperature (oC) I 24.91 24.61 25.01 24.8 Alkalinity (mg/L CaCO,) DO m IL 7.81 7.4 Conductivity (wnhoslcm) 297 '" (SU) I 7.891 7.861 7.961 7.78 '" (SUI I 7.921 7.901 8.021 7.86 Temperature ('C) I 25.01 24.61 25.01 24.6 DO m L '.7 Couductivi mhos/em Alkalinity (mgIL CaC03) Hardness (mgIL CaC03) Total Residual Chlorine m Temperature (0e) DO (mg!L) I 791 791 781 7.6 Conductivity (umhos/em) 291 Iii* -nti 284 !jjW:=§I" Hardness (mgIL CaC03) '" ISUI I 7.891 7.861 7.981 7.78 ,Temoerature ('C) '" ISU) I 7.891 7.901 7.991 7.82 '" Ism I 7.851 7.901 7.921 7.79 DO (mgIL) I 791 801 781 7.6 Conduetivitv ClLIDhoslcml 2651!i!'": 71'; 259_I Temperature r'C) I 24.91 24.91 25.01 24.7 Alkalinity (mg/L CaCOJ) 63 I u,) Hardness (mgIL CaC03) 88 Temperature flC) 24.9 DO m Conductivity (umbos/em) no m Conductivity (umhos/em) 100% 86.4% 43.2% 21.6% 10.8% Control 100% Intake File: sqn101_050812chem.xls Entered by: C. Johnson Reviewed by:-Jf--

Page 41 of 110 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Analyst Page I of7 Connty: Hamilton Ontfall: 101 Sample numbers used Outfall 101 Intake MHSW batch used Comments: 10,8 21.6 432 86.4 ' 100 270 540 1080 2160 2500 2230 1960 1420 340 0 2500 2500 2500 ,2500 2500 Test initiation and feeding, renewal and feeding, or termination time Date o 2 3 Daily renewal information: 4 Client: Tennessee Valley Authority Facility: Seguoyah Nuclear Plant NPDES #: TN0026450 Project #: , &1$, 7 5 6 Day Test orf!anism source information: Test information: Organism aile: < 24-hours old Randomizing temvlate color: &iI...t> Date and times organisms were born .0t.*08- \\'1.;. 0..'\\0 'll> O~* Incubator number and shelf between: location: 1..c..\\ Culture board: OS-O\\'\\'1-Replicate number: 1 I 2 1 3 41 5 1 6 1,71,8/9/'" YWT batch: t/S'O\\o 1'1.;. Culture board cun number: 1ll 1'IJi hI. ~ I!ll. I\\.; I~ I '!>\\I \\10 !'I 0 Transfer vessel infonnation: I}H,

  • S.U.

Temnerature t(..\\ °C Se/enastrum batch: (),\\.~.\\'L Average t11U1sfer volume (mL): I (). ~'2.1-.l4 Control information: Summary oftest endpoints: Control-! Control-2 Accentance criteria % ofMale Adults: M. 'r- 07.

520%

7-day LC,o ') IDD'1. % Adults having 3'" Broods: I 1n1. iiOf" >80% NOEC In"7. % Mortality: 101.

20%

LOEC 'T 101\\7. Mean OffsorinlZlFemale: 3\\.1 -!oo.r,. ~ 15-.0 offspring/female ChV '> 1()().1. %CV: lo :17. Io*n "<40.0% IC" >1007. Ind""endent Ro~.",by "'~"" SOP ATII - Exhibit AT11.2, revision 06-01-11

Page 42 of 110 Page 2 of7 Concentration: % Mortality: I 0". Mean OffsorilllzlFemale: I.'n.", SOP ATlI - Exhibit ATlI.2, revision 06-01-11 Survival and ReproductIOn Data Species: Ceriodaphnia dubia Client: TVA I Segnoyah Nnelear Plant. OntfalllOI Date: OS. 08.. 11-CONTROL-l SurVival andReproduction Data No/e. Adult mortality (L hve, D dead), 58 split brood (smgle brood spltt between two days), CO carry over (offspnng earned over WIth adult dunng transfer). CONC' 108% Replicate nnmber Dav 1 2 3 4 5 6 7 8 9 10 1 Young produced () 0 0 0 0 0 0 a u (:) Adult mortality L L-L- L-I..- L-L l-L. L 2 Young produced j) D j) () j) 0 0 0 0 0 Adult mortality L L-L L-L L \\.- \\.- 3 Young produced D D 0 0 C:J (\\. C> 0 0 C> Adult mortality L \\.- L-L- L. \\... L L... 4 Young produced '"-\\ ~ '-l l., '-l. c::. S '-I Adult mortality L... \\..-. L. L... L \\..- '-- L. 5 Young produced \\'1_ \\-:a.. \\() n ,~ IL. 17., \\"1_ l~ Ih Adult mortality L \\..- L. L L-L L L 6 Voung produced (} D a 0 0 0 0 0 0 () Adult mortality L L-L- L L. L L... L L L-7 Young produced I' \\e... \\~ I\\. ,'-I IS . \\i \\"'\\ I"" Total young produced O~ 30'2. 3b ~I

!>Io.

31:>

!I!:l 3"\\

3\\ '2.'\\ Final Adult Mortalitv L \\... \\..- \\..:..- \\.... X for 3rd Broods )(. X. 'V' ')( X. >Co ~ ~ )C . '£ ReDlicate number Day 1 2 3 4 5

  • 6 7

8 9 10 1 ¥ol;lng produced () i\\ f"\\ (i 0 C C (j (') 1(") Adult mortality L L-L. L L L l L 2 Young*produced (') 0 D 0 a 0 .tl C\\ (') 0 Adult mortality l... L L L l... L L L l... L.. 3 Young produced 0 C> 0 (:) () 0 0 n tl 0 Ad~lt mortality \\..- L.. L- \\... l-L L L L L-4 Young produced 1.\\ ~ S .3 \\.\\ \\.\\ .s Adult mortality L-L.. L L.. L.. L.. L L. 5 Young produced \\1. \\~ \\1. \\'1_ 1'2,) \\ "'L 10 12.. I~ \\ 1-Adult mortality L L L L L L L.. \\... 6 Young produced () D 0 0 0 0 0 0 0 0 Adult mortality L L.. L L L L.. L L L. L. 7 Young produced n \\"" ,~ \\10 \\5 \\\\0, \\'-\\ 1\\ 11 \\S Total young produced 3>3 ~!:l 1>b !l~ .!l\\ ~1. '22 as 1>S .!>1.... Final Adult Mortality \\. \\... L L L \\..-. Note: Adult mortality (L live, D dead), 58 ~ split brood (single brood splil between two days), CO c over (off's rin clUried over with adult durin transfer). Concentration: % Mortality: 0 Mean Offspring/Female: .,.,.~ % Reduction from Control-l: -0,,7. '"d.p,"~'"' Rovlowby .,II*V£.K.",** "L I I I I I I I I I I

Page 43 of 110 Page 3 of7 Survival and Reproduction Data Species: Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant, Outfall 101 Date: O$./lt"I'1_ CONC' 21 6% Su,..,ival and Reproduction Data °ETS ~ '." I:

)

\\~'""<:: If 'h',,~'j~ ~ EJWlnlnment,lTeSllngSeluUonr.-llI<. CONC' 432% , Renlicate number Dav 1 2 3 5 6 7 8 9 10 1 Young produced a 0 0 a D 0 [) c, 0 IJ Adult mortality \\.... l-l-L- L-L- L.. L-L.- L.. 2 Young produced 0 0 0 0 0 0 0 0 0 0 Adult mortality L L-L l-L- L..- L. I-L L.. 3 Young produced (") 0 0 D 0 () (') 0 C\\ ("} Adult mortality L-L- \\.... \\.... L- \\... L-L. \\... L 4 Young produced "'l \\0 S S '-\\ 5 .C; ~ Adult mortality \\.... "- I-l- I..... 5 Young produced \\~ \\ "l... 1'--\\ ~~ \\\\ \\.", I'l... \\2. \\5, \\'2_ Adult mortality \\.... '- L \\..... \\..... L L 6 Young produced a 0 0 a () 0 0 0 () (') Adult mortality L L L. L. L L- \\.... L-L L 7 Young produced \\"1 II.. ~\\. IS Ie, '5 .1Ii \\So I'" \\\\0 Total young produced

!l\\.
,'1.

~\\" .;~ ~\\ ~'2. ~S .:'>2. as ~ Final Adult Mortality "L L. L-L. L. \\,... \\,... Note: Adull mortality (L ~ live, D dead), 58 = split brood (single brood split between two days), CO = c over am rin carried over with adult durin transfer). Concentration: % Mortality: f'l1. Mean OffspringIFemale: a,.<.. % Reduction from Control-I:

  • S.01.

Replicate number Dav 1 2 3 4 5 6 7 8 9 10 1 Young produced n (') () (j 0 () () 0 0 (') Adult mortality L. L. L. L-L. L L L L. L.- 2 Young produced .(") 0 0 n 0 (") () () () (') Adult mortality L L. L. L L. L .L L L L. 3 Young produced () () (') () D () 0 0 0 0 Adult mortality L L L L L... I-L L L L..... 4 Young produced 4 '-I 4 \\. '-l '-\\ \\0 So 5; Adult mortality \\.... \\.... L... L L. L..... '- '- L. 5 Young produced 1Y. L1... \\4 \\'-\\ \\""'L l'l..... 1\\ \\~ I"'L \\~ J\\dult mortality. \\.... L.. \\.... \\..... \\..... L-L L... 6 Young produced 1"\\ ('") () 0 0 0 0 U u (") Adult mortality L L L L L. L L L L L.. 7 Young produced \\'1. \\<; \\S \\"\\ n \\,,\\ 1& \\'\\ 1\\0. \\'5 Total young produced !>S "3\\ .3~ 31 3.3 3b .35 31 ..3.3 33 Final Adult Mortality L... L.. L.. Note: Adult mortality {L = live, D dead),58 split brood (single brood split between two days), CO ca over offs rin carned over with adult durin transfer). Concentration: % Mortality: 07. Mean OffspringIFemale: ~.C; % Reduction from Control-I: -(,,37.. l In;epend,nt R,lIIewlly t<eIIOy!.Keo..n ~ SOP ATI I - Exhibit ATI 1.2, revision 06-0I-II

Page 44 of 110 Page 4 of7 Survival andReproduction Data Species: Ceriodaphnia dubia Client: TVA I Seguoyah Nnclear Plant. Outfall 101 Date: 0$.0'-11.. CONC' 864% Survival and Reproduction Data CONC' 100% RenJicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced (') () f\\ t\\ f\\ (j (') 11 0 0 Adult mortality L \\.. L L L. L L L L. L 2 Young produced 0 D () 0 () (") 0 0 (j 0 Adult mortality L L L L. L... L L L 3 Young produced () 0 D f) (') r) 0 0 0 (') Adult mortality L L L L L.. \\.- L L ~ L.. 4 Young produced ~ '4 '-\\ ~ 'E:. l." ~ S Adult mort~lity \\... L L... L L... L \\-.. 5 Young produced '0 \\'2... '"b \\.... I~ ,~ \\"L ,~ l"'l Adult mortality ~ L. L L L. L.. L L L L 6 Young produced 0 0 0 0 (\\ 0 0 a u (J Adult mortality L L L L. \\.... L L "L.. L. 7 Young produced \\8 L"l II.> I" 20 I~ \\5 Ig 11 Total young produced 31. 'SI. ?IS as 31 3~ ~ ~c.. ?IS 310 Final Adult Mortality L \\.... L L. L L \\.... L Note: Adult monality (L live, D ~ dead), S8 split brood (single brood split between two days), CO over offs rin carried over with adult durin transfer). Concentration: % Mortality: 01 Mean Offspring/Female: .~C:. p % Reduction from Control-I: ~I2.17. ReDlicate number Dav 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 a (") (") 0 0 () Cl 0 0 Adult mortality l L L. L. L. L-L L-L L.. 2 Young produced 0 f) 0 () r, () () 0 0 0 Adult mortality L L L.. L. L L. L.. L.. L L.. 3 Young produced (") () 0 0 D 0 0 0 0 C\\ Adult mortality L L. L-L- l.- . \\.-. '- L L-4 Young produced c:.. '-l S \\0 <4 ~ '-\\ \\0 Adult mortality L.. L L.. L- \\')., \\b 'S \\b \\~ \\ 'L 5 Young produced l"L. , t \\, 17-Adult mortality L L... L L L... L L. L 6 Young produced 0 D () () a b 0 0 0 Adult mortality L L. \\. L L. L. L. L L L 7 Young produced I' \\& IS \\~ 1'\\ I'" 1'\\ \\1 n 11 Total young produced .!I'-l 31. 3'1. 35 31 35 3'-\\ ~'-\\ ~~ 35 Final Adult Mortality \\. L \\.... \\... L.. Note: Adult monality. (L '" live, D dead), S8 = split brood (single brood split between two days), CO "Cllf' over (offsprin~ carried over' with adult durin transfer). Concentration: % Mortality: N. Mean Offspring/Female:

!I't.S

% Reduction from ControJ*I: -g. '2.7. j!'c,:;'cee' 'l Ind.".n.'" Re\\1ewb~ K.II'VE.K,.nan Inhf SOP ATil - Exhibit ATl1.2, revision 06-0I-II

Page 45 of 110 Page 5 of7 Concentration: % Mortality: I D1. Mean Offspring/Female: I *':f,O.10 SOP ATil - ExhIbIt ATl1.2, reVISIOn 06*01 -11 Survival andReproduction Data Species: Ceriodaphnia dubia Client: TVA I Seguoyah Nuclear Plant, Outfall 101 Date: CONTROL-2 Survival andReproduction Data Nate. Adult mortality (L hve, D dead). S8 split brood (smgle brood spilt between two days), CO carry over (offspnng earned over WIth adult dunng transfer). CONC' 100% Intake Replicate number Dav 1 2 3 4 5 6 7 8 9 10 1 Young produced (J () (J (J C) (') (') n () () Adult mortality 1 \\...... L \\...... L L. L .L-L.. 2 Young produced 0 0 0 0 0 0 0 0 0 () Adult mortality \\...... \\...... L \\...... L. L L L-L:- 3 Young produced ("") 0 0 r-, () 0 0 Cl 0 n Adult mortality L \\..- L.. L L. L.... L 4 Young produced '-l \\.. S 4 4 "-I. '-\\ '-I 3-Adult mortality \\....... I-L L L L L "~ 5 Young produced \\{:) I~ \\"'L 11. \\.7\\ 1\\ \\ \\ 1\\ \\ \\ Ib Adult mortality \\.... L L. \\. \\... L 6 Young produced 'f") (', 0 0 0 (:; 0 0 () 0 Adult mortality \\.... L L L-L. L L.. L 7 Young produced \\10 \\L.\\ ~"" 14 1'-\\ l~ ~'l 11. 11. \\~ Total young produced 30 3~ 3~ 3b 3\\ '2.'; 32- .3\\ 31 2.r.. Final Adult Mortalitv L L L.. L.. l \\.... L \\. X for 3r (l Broods )C.... X ~ x. .x... '7'-

c...

)C... X )<. Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced n f\\ r. n n (') (\\ () (') 0 Adult mortality L L L 1 1 L L L L l-2 Young produced () () n 0 0 (J .() 0 a 0 Adult mortality L L L L L. L. L L L L 3 Young produced (j 0 0 0 0 (J 0 () 0 () Adult mortality L L L L L L L L L.. L. 4 Young produced '"\\ S '-\\ .s \\" (., lo '-l S Adul~ mortality L L.. \\.... L.. l-I.... I.,- 'L.. 5 Young produced \\b \\~ 10 1'-\\ I':!:. 1"2... I~ ID n.. I \\ Adult mortality L L L L.. L.. L.. 6 Young produced (') 0 () () 0 0 0 () 0 0 Adult mortality L L L- '- L L L L L L. 7 Young produced II. I' 14 \\4 I~ \\l2 \\3 \\S I' \\5 Total young produced

33. as 2"1 3"t 3\\

31. ..3'2. 3\\ 3~ ..3\\ Final Adult Mortality \\.. L L L \\... ~ L Note: Adult mortality (L live, 0 dead). SB split brood (single brood split between two days), CO over off!; rin carried over with adult durin transfer. Concentration: % Mortality: 07. Mean OffspringIFemale: 3t*'!. % Reduction from ControI~2: -S.r..7- ,"d.pen~.M RovIowby I<elloyE,~""" I~ ~ I I I I I I I

Page 46 of 110 I'SlH ~;!l ~ETS ~ Envlronm..ntal TesUng SolutIons, Inc. TVA I Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 08-15, 2012 Verification of Ceriodaphnia Reproduction Totals 86.4% -~ Day Re Iicate number Total I 2 3 4 5

  • 7 8

9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 5 5 4 5 5 6

  • 4 4

6 48 5 12 13 13 IS 13 13 11 13 12 12 127 0 0 0 0 0 0 0 0 0 0 0 7 17 18 IS IS 19 16 19 17 17 17 170 Total 34 3. 32 35 37 35 34 34 33 35 345 Control-l Day Re Iicate number Total I 2 3 4 5

  • 7 8

9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4

  • 4 5

5 4 5 4. 5 12 13 10 12 14 12 13 12 13 10 121 0 0 0 0 0 0 0 0 0 0 0 7 17 IS IS IS 16 14 IS 17 14 14 152 Total 33 32 30 31 3. 30 33 34 31 29 319 100% Day Re licate Dumber Total I 2 3 4 5

  • 7 8

9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 5 6 4 4 5 5 6 4 5 50 5 13 12 13 12 13 IS 13 12 14 14 131 6 0 0 0 0 0 0 0 0 0 0 0 7 18 19 16 19 20 18 IS 18 17 17 177 Total 37 36 35 35 37 38 33 36 35 3. 358 10.8% Day Re licate Dumber Total 1 2 3 4 5

  • 7 8

9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 5 3 4 4 6 5 5 45 5 12 13 12 12 13 12 10 12 13 12 121 6 0 0 0 0 0 0 0 0 0 0 0 7 17 16 13 16 IS 16 14 17 17 IS 1S6 Total 33 33 30 33 31 32 28 35 35 32 322 Coutrol-2 Day Re Iicate number Total 1 2 3

  • 5
  • 7 8

9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 6 5 4 4 5 *

  • 4 3

43 5 10 13 12 12 13 11 11 11 11 10 11. 6 0 0 0 0 0 0 0 0 0 0 0 7 16 14 16 14 14 13 17 16 16 13 149 Total 30 33 33 30 31 29 32 31 31 26 306 21.6% -~.- -- Day Re licate number Total 1 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 6 5 5 4 5 5 4 5 47 5 13 12 14 13 11 13 12 12 IS 12 127 0 0 0 0 0 0 0 0 0 0 0 7 19 16 16 IS IS IS 18 IS 16 16 161 Total 36 32 36 33 31 32 35 32 35 33 335 100% Iutake Day Re licate number Total 1 2 3 4 5

  • 7 8

9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 5 6 6 6 4 5 49 5 13 14 10 14 13 12 13 10 12 11 122 6 0 0 0 0 0 0 0 0 0 0 0 7 16 17 14 14 13 18 13 IS 17 IS 1S2 Total 33 35 29 32 31 36 32 31 33 31 323 43.2% Day Re licate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 6 4 4 6 5 5 5 47 5 14 12 14 14 12 12 11 13 12 13 127 0 0 0 0 0 0 0 0 0 0 0 7 17 IS IS 19 17 14 18 19 16 IS 165 Total 35 31 33 39 33 30 35 37 33 33 339 File: sqn101_050812data.xlsx Entered by: J. s~ner Reviewed by: ~

Page 47 of 110 !=.. T..r. 1~'.*:.:\\ ~ ~ I ~H {~f} CD @o ~ Environmental Testing Solutions, Inc. TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 08-15, 2012 Ceriodaphnia dubia Chronic Whole Efflnent Toxicity Test EPA-821-R-02-013, Method 1002.0 Qnality Control Verification of Data Entry, Calcnlations, and Statistical Analyses 7875 Project number' ,~.~ Concentration Reolicate number Survival Average reproduction Coefficient of Percent reduction from (%) (Ufo) (offspring/female) variation ('Yo) control (%) 1 2 3 4 5 6 7 8 9 10 Control-I 33 32 30 31 36 30 33 34 31 29 100 31.9 6.7 Not applicable 10.8% 33 33 30 33 31 32 28 35 35 32 100 32.2 6.7 -0.9 21.6% 36 32 36 33 31 32 35 32 35 33 100 33.5 5.5 -5.0 43.2% 35 31 33 39 33 30 35 37 33 33 100 33.9 7.9 -6.3 86.4% 34 36 32 35 37 35 34 34 33 35 100 34.5 4.2 -8.2 100% 37 36 35 35 37 38 33 36 35 36 100 35.8 3.9 -12.2 Control-2 30 33 33 30 31 29 32 31 31 26 100 30.6 6.8 Not applicable 100% Intake 33 35 29 32 31 36 32 31 33 31 100 32.3 6.4 -5.6 Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnett's MSD value: PMSD: 2.036 6.4 1.599 5.2 MSD~ PMSD= Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound detennined by USEPA (lOlh percentile) = 13%. Upper PMSD bound detennined by USEPA (90th percentile) = 47%. Lower and upper PMSD bounds were detennined from the 10th and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 200Ib). USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-Q0-Q03. US Environmental Protection Agency, Cincinnati, OR. USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study ofEPA Short~tenn Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA~821-B-OI-004 and EPA-821-B-Ol-005. US Environmental Protection Agency, Cincinnati, OH. File: sqn101_050812data.xlsx Table populated from associated Verification of Ceriodaphnia Reproduction Totals" spreadsheet. Spreadsheet entered by: J. Sumner Reviewed by:-0\\---

Page 48 of 110 ~~T£ ~ EnvlronmonlBlTosIl"!I 501"'lon.. Inc. TVA I Sequoyah Nuclear Plant, Outfall 101 Non-treated May 08-15, 2012 Statistical Analyses Cerlodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/8/2012 Test ID: CdFRCR Sample 10: TVA/SON 101 End Date: 5/15/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2012 Protocol: FWCHR-EPA-821-R-02*013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-% 1 2 3 4 5 6 7 8 9 1a Control~1 33.000 32.000 30.000 31.000 36.000 30.000 33.000 34.000 31.000 29.000 Control~2 30.000 33.000 33.000 30.000 31.000 29.000 32.000 31.000 31.000 26.000 10.8 33.000 33.000 30.000 33.000 31.000 32.000 28.000 35.000 35.000 32.000 21.6 36.000 32.000 36.000 33.000 31.000 32.000 35.000 32.000 35.000 33.000 43.2 35.000 31.000 33.000 39.000 33.000 30.000 35.000 37.000 33.000 33.000 86.4 34.000 36.000 32.000 35.000 37.000 35.000 34.000 34.000 33.000 35.000 laa 37.000 36.000 35.000 35.000 37.000 38.000 33.000 36.000 35.000 36.000 Intake 33.000 35.000 29.000 32.000 31.000 36.000 32.000 31.000 33.000 31.000 Transform: Untransformed i-Tailed Isotonic Conc-% Mean N*Mean Mean Min Max CV% N t*Stat Critical MSD Mean N-Mean Control-1 31.900 1.0425 31.900 29.000 36.000 6.683 la 33.633 1.0000 Control-2 30.600 1.0000 30.600 26.000 33.000 6.750 la 10.8 32.200 1.0523 32.200 28.000 35.000 6.677 la -0.337 2.287 2.036 33.633 1.0000 21.6 33.500 1.0948 33.500 31.000 36.000 5.495 1a -1.797 2.287 2.036 33.633 1.0000 43.2 33.900 1.1078 33.900 30.000 39.000 7.921 la -2.246 2.287 2.036 33.633 1.0000 86.4 34.500 1.1275 34.500 32.000 37.000 4.156 la ~2.920 2.287 2.036 33.633 1.0000 100 35.800 1.1699 35.800 33.000 38.000 3.906 10 -4.381 2.287 2.036 33.633 1.0000 Intake 32.300 1.0556 32.300 29.000 36.000 6.370 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.68163 1.035 0.20651 0.0673 Bartlett's Test indicates equal variances (p = 0.37) 5.43364 15.0863 The control means are not significantly different (p = 0.18) 1.38493 2.10092 Hypothesis Test (i-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 2.03577 0.06382 21.1867 3.96296 4.6E-04 5,54 Treatments vs Control~1 Point SO Linear Interpolation (200 Resamples) 95% CL Skew 150 50 100 Dose % 1.0.,------------, 0.9 0.8 0.7 0.6 ~ 0.5 &. 0.4 ~ 0.3 a.' 0.1 0.0 f-o-;+---'<>---+.... -0.1 -a.' -h_~~~_~_~--.-I a >100 >100 >100 >100 >100 >100 >100 IC05 rC10 IC15 IC20 IC25 IC40 IC50 Dose-Response Plot 45 40 35 0 30 ~ 25 "i 20 II: 15 10 5 0 N ~ ~ a £ ~ N w.. 0 0 1-tall, 0.05 level of signlflcance is Ind.p.nd.n' R,vi'wby K.II..,E.K....n ~" File: sqnlOl_050812dataxlsx Entered by: J. StulUler ReviewedbY:~

Page 49 of 110 eElS

    • o ""

o '" ~ EnvIronmental Testing Solutions, rnc. TVA / Sequoyah Nuclear Plant, Intake Non-treated May 08-15, 2012 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 51812012 Test 10: CdFRCR Sample 10: TVA 1SON 101 - Intake End Date: 511512012 Lab 10: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-% 1 2 3 4 5 6 7 8 9 10 Control-1 33.000 32.000 30.000 31.000 36.000 30.000 33.000 34.000 31.000 29.000 Control-2 30.000 33.000 33.000 30.000 31.000 29.000 32.000 31.000 31.000 26.000 10.8 33.000 33.000 30.000 33.000 31.000 32.000 28.000 35.000 35.000 32.000 21.6 36.000 32.000 36.000 33.000 31.000 32.000 35.000 32.000 35.000 33.000 43.2 35.000 31.000 33.000 39.000 33.000 30.000 35.000 37.000 33.000 33.000 86.4 34.000 36.000 32.000 35.000 37.000 35.000 34.000 34.000 33.000 35.000 100 37.000 36.000 35.000 35.000 37.000 38.000 33.000 36.000 35.000 36.000 Intake 33.000 35.000 29.000 32.000 31.000 36.000 32.000 31.000 33.000 31.000 Transform: Untransformed i-Tailed Conc-% Mean NwMean Mean Min Max CV% N t-Stat Critical MSD Control-1 31.900 1.0425 31.900 29.000 36.000 6.683 10 Control-2 30.600 1.0000 30.600 26.000 33.000 6.750 10 10.8 32.200 1.0523 32.200 28.000 35.000 6.677 10 21.6 33.500 1.0948 33.500 31.000 36.000 5.495 10 43.2 33.900 1.1078 33.900 30.000 39.000 7.921 10 86.4 34.500 1.1275 34.500 32.000 37.000 4.156 10 100 35.800 1.1699 35.800 33.000 38.000 3.906 10 Intake 32.300 1.0556 32.300 29.000 36.000 6.370 10 -1.844 1.734 1.599 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97072 0.868 -0.3101 0.44033 F-Test indicates equal variances (p = 0.99) 1.00787 6.54109 The control means are not significantly different Cp = 0.18) 1.38493 2.10092 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.59873 0.05225 14.45 4.25 0.08172 1, 18 Treatments vs Control-2 Dose-Response Plot 45,---------------------, 40 35 10 5 T T-.I T T.L .L 1----...,

1---- ----------------------------

1-tail, 0.05 level of significance In,,"pend,nl ~-. ~'I"~""" 0 g ~ 0 ii g 0 OJ 0 ~ ~ l'l c c 8 0" File: sqn101_050812data.xlsx Entered by: 1. S~er Reviewed by: ~

Page 50 of 110 Species: Ceriodophnio dubio Client: TyA I Segunyah Nuclear Plant. Outfall 101 Doil Chemist Page 6 of7 Date: OS-O1].. SOP ATIl - Exhibit ATI1.2, revision 06-01-11 11 &\\ Initia~ 43.2% 21.6% Anal st 100% 10.8% 86.4% 100% Intake CONTROL Concentration Parameter Indep<ndO<1l "vi,wb, K.IIII'/~.K**",," I~

Page 51 of 110 I ."th i*e. I I I I I I I I I I I I I I I I I Species: Ceriodaphnia dubia Client: TVA / Seguoyah Nuclear Plant. Outfall 101 Concen-Parameter tration CONTROL 10.8% 21.6% 43.2% 86.4% 100% 100% lutake Page 7 of7 Date: 0$.01-11... SOP AT! 1-Exhibit AT! 1.2, revision 06-01-11

Page 52 of 110 7875 Project Dumber:-----'-'--'- Daily Chemical Analyses Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 08-15, 2012 ~ ~Jm ,eElS + ,-.~.~ ~ '"ct. !1i" ./"\\' () Environmental Testing Solutions, Inc. 8.0 7.85 8.0 7.86 25.0 24.9 7.7 2581's,. 7.7 212l.i' 7.7 280lfi 24.8 8.10 8.09 Dav3 7.7 2941",: 8.10 ~ 76 1 ~ 294~~j*.. JV7 ; 63 ";"',",',',-,.....,......- 8.07 24.8 24.8 ...E. 24.8 Initial Final 7.98 8.02 1 T Da 2 Final I Initial Final 7.961 7.95 8.0iT 7.7 7.81 303 63W m n 89liiliiif11 1M' 25.0 25.1 24.9 24.9 7.96 7.97 7.90 8.01 7.8 7.6 7.8 7.8 297 292, i 25.2 24.8 25.0 24.9 7.98 7.97 7.92 8.01 7.8 7.6 7.8 7.8 284 280"'ill 25.2 25.1 25.0 24.91 7.99 7.97 7.91 8.02 7.8 7.6 7.8 7.81 259_I. 2577 i I 25.2 25.2 25.0 25.0 8.01 8.01 7.88 8.03 7.9 7.6 7.9 7.8 210 lIJlJ i1L!ilt 211 iIII -- 25.2 25.0 25.0 8.04 7.89 8.06 7.9 7.8 197 ",-,~'==-",,= 7.99 8.0 8.1 25.2 7.95 252 DO(m~ DO(mgl ) Conductivity (J,UIlhoslcm Conductivity (lUDboslcm Temperature f'C) U(s* H(SU IRes H(SU Temperature (Oq Conductivity (umhos/em' DOlmgJ, Conductivity (umbos/em Alkalinity (mglL CaC03) Temperature (0e) DO (mglLl Conductivity (Jlmhos/em oR(S Hardness em-gIL CaC03) Total Residual Chlorine (m~ iH (SU DO (mglL) Conductivity (umbos/em Temoerature (0C) Temperature ('C) Hardness (mgIL CaCOJ ) Conductivity (llIDhoslcm !DOCm: ITemperature ('I ~AlkaliDity (mgIL CaC03) 21.6% 100% 86.4% 43.2% 10.8% Control DO(m: H(SU Temperature (Oq Total Residual Chlorine (ml!!L' 100% Intake IAlkalinity (mgIL CaC03) I // Hardness (mglL CaCO,) Concentration IParameter File: sqn101_050812chem.xls Entered by: C. Johnson Reviewed by:-a\\-

Page 53 of 110 Dilution preparation information: Comments: Dilution prep (%) 10.8 21.6 43.2 86.4 100 Elich concentration was UV-treated Effluent volume (mL) 270 540 1080 2160 2500 for 2 minutes to remove pathogenic Diluent volume (mL) 2230 1960 1420 340 0 Interferences. Total volume (mL) 2500 2500 2500 2500 2500 Test orl!anism information: Test information: Omanism a~e: 1'1.'1.\\,.,n<- l\\~ Randomizing template: -e.L\\)-L Date and times organisms o~o 0"\\0n. \\I0I:>O Incubator number and .?>e. were born between: shelf location: Organism source: ~~ ~,t.~\\ (>f OS-Cf\\-rL Artemia CHM number: ell'""c;"z. Drying informationfor weight determination: Transfer bowl information: pH= '.1"1 S.U. Date / Time in oven: ..... 1...I'\\.. Temoerature = "L'\\. f/ 'c Initial oyen temoerature: 101'\\*C. Average transfer volume: O. \\1..0'l-Jl Date / Time out of oven: IM-.IL*\\'\\.. I Final oven temperature: bo't Total drying time: l.",.I\\oolt~ County: Rhea Outfall: 101 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Seguoyah Nuclear Plant NPDES #: TN0020168 Project #: __'...&...1....5........ Dailyfeeding and renewal information: Page 10f6 \\'10 MHSW batch used Sample numbers used SOP AT20 - Exhibit AT20.3, revision 06-01-11 Test initiation, renewal, or termination Acceptance criteria 61. Afternoon feeding Morning feeding Date Control in ormation: % MorlaH : Average weight per initial larvae: Average weight per surviving larvae: Time Anal st Time Anal st Time Anal st Outfall 101 Intake 0 IS'-lS 1'\\00 2 14~ 3 1"'00 4 11.\\00 5 .'100 6 l"leo 7 Independe"' 1Ie";,wby KoIIO'/E.K..nan 'VL Day ~ ~ ~ I I I

Page 54 of 110 l\\o~owbV

    • n.V!.*'*""*

lie SOP Ar20 - Exhibit Ar20.3, revision 06*01*11 Day CONTROL 10.8% 21.6% A B C D E F G* H I J K L 0 10 10 /0 10 10 '/0 '. /0 10 10 I(J '0 10 1 It> It> I(J 10 It> 10 10 10 10 10 10 10 2 10 ID 10 10 ID 10 It;) 10 10 10 I() 10 3 10 /0 10 10 10 It> 10 10 10 10 1O 10 4 10 10 Ie 10 10 /0 10 10 10 10 /0 10 5 Ie 10 10 /0 10 ./0 10 10 10 10 10 to 6 /Q It> 10 to It;) 10 10 10 10 10 10 10 7 tt',) It> /0 /0 10 10 IC Ie 10 10 10 IQ A - Pan weight (mg) Tray color code:: ~Hw... 13.H I!.o;t. 14-.I~ I';.t; 14-.3~ 5.'14 1'5.8'\\ I~.~f 14.1'1. r!>...4 1~.1'J I+.or. Analyst: Date: DS'QlAY B - Pan + Larv~ht (mg) . 14.118 20,03,'t..~O 1't:Z."f 1J),~ 20.15 2.0.115 Zo.'Iz. 14.16,q.llp,,,,z.1.J 20,111 Analyst: Date: ~I:ut C - Larvae weight (mg) - B - A Hand calculated. AI, ~.'\\\\ Co," s.a1 ~,~I\\ Co.~1....'2:0 ....\\10 Io,lta s.~ fo.11. !",SCI l..~ Analyst: v Weight per initial number of larvae (mg) = C I Initial number of larvae 0-. rv' ~ 4' to' ~o-.. Il, ~

f
',J'

.0\\,. Sf ~ i:! Hand calculated*..)' ~ '0 \\0 \\0 c.; \\y O' (r Analyst: O* 0' O* O' O' 0' I)' \\)" O' Average weight per Percent reduction O.l.t.\\S - I{J.l7,. O.l.'2.~ - '",'S.7. initial number of from control (%) O. 'S&Co larvae (mo) Survival and Growth Data Page 2 of6 Date: oS ",8' \\ 1-Species: Pimephales promelas Client: TVA / Seguoyah Nuclear Plant, Outfall 101, UV-treated Comments: Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk =sick, sm =unusually small, 19 =unusually large, d&r = decanted and returned, w=wounded. ~ EnvllllnmentlllTesting Solutions, Inc. ~ I I I I I

Page 55 of 110 iET ~ EnvfronmentalTestJng Salutions,.Jnc. Species: Pimephales promelas Client: TVA I Segnoyah Nuclear Plant, Out(alll0l, UV-treated Date: oS'O~, 11,... Page 3 of6 Survival and Growth Data Day 43.2% 86.4% 100% M N 0 p Q R S T U V W X 0 ID ID 10 10 10 It; /0 to /0 10 /0 /0 1 10 10 /0 10 10 10 10 /0 10 10 10 10 2 10 10 It> It> Ie 10 to 10 1O 10 ID 10 3 /0 /0 /0 10 10 fO 10 fO fO fO 10 10 4 /0 10 10 10 10 /0 (t> ID 10 (0 10 I'D 5 /0 10 Ie I'D ID 10 10 /0 10 to to /0 6 to 10 /0 10 to (0 10 (0 10 ID 10 10 7 tD 10 /0 /0 I(J 10 10 IQ 10 10 It) JD A = Pan weight (mg) Tray color code:: ~ 14.41. \\'3.'\\3 1!l.St 14:~'5 1!l.tI. IMo \\'3.'31. 14.~\\ I3*SIl 14.42-14.0:} 1'3.I~ Analyst: M Date: °5*ot.*\\1.. B ~ Pan + Larv:r{ (lOg) 1'1.59 1'1.111 21.2.tt /'I.5Z l'l.~ Zo.lCil 1'I,lflt 1'1.51 2,O,Sl %.1.22-20.03 j'l.1,5 Analyst: ~ Date: OJ.I~ C - Larvae weight (mg) - B - A Hand calculated. J _ S.t.'i 10.0'\\,,:!><.\\ 10.010 5.!>1 S*1!> 10.10 5.100 (,.1!l ",ISO 10.1<" "',S!> Analyst: 0... Weight per initial number of larvae (mg) = C I Initial number of larvae ~:.., ~\\) 'DO ,,"":J.J.O ~ ~ 'lDt.., ~

t

~ Hand calculated*. 01 ~ '16 '16 ~ \\0 O' \\0 O' O' O' 'i:i ()' \\l' b' \\). o* c' O' Analyst: Average weight per Percent reduction O.t..O'-l 2..17. 0.1011

  • 1"'.~lt initial number of from control (%)

-3.01. O.S1b larvae (100) Comment codes: c = clear. d =dead, fg =fungus. k =kiIled, m =missing, sk =sick, sm ~ unusually small, Ig ~ unusually large, d&r ~ decanted and returned, w =wounded. Comments: SOP AT20 - Exhibit AT20.3, revision 06-01-11

Page 56 of 110 Species: Pimephales promelas Client: TVA I Seguoyah Nuclear Plant, OutCa11101, UV-treated Page 4 of6 Date: Survival and Growth Data 100% Intake y Z AA BB ID 10 to 10 10 10 /0 /0 to 10 q,Q. ID 10 Ie "\\ (0 10 10 &\\ LO 10 IU q 10 10 "It II) ~ 10 (0 13.t, 13.8; IS.7.,\\ IQ,14l IQ.v.i 20,1'0 S!l1 s.'\\'l. S,\\'\\ o-'V ~ tt t? ~ ~ 0' I)' o' Q" 0,(,,1"1 - '"\\.17. o Day 6 C = Larvae weight (mg) = B - A A = Pan weight (mg) Tray color code:: t1\\~~'a.. Analyst: I't\\~ Date: os..ot.* \\1.- 7 2 1 4 B = Pan + Lar~e weOght (mg) Analyst: Dale: 06.1: Hand calCulatedy Analyst: C3-l 3 5 Average weight per Percent reduction initial number of from control (%) larvae em ) Weight per initial number oflarvae (mg) = C I,Initial number of larvae Hand calculated.y Analyst: e I Comment codes: c ~ clear, d ~ dead, fg ~ fungus, k ~ killed, m =missing, sk ~ sick, sm ~ unusually small, 19 ~ unusually large, d&r = decanted and retumed, w ~ wounded. Comments: Inaeperl<!e"' Rel'i,w~y K.IIO'/E.~..na" 1A.: 1 S: SOP AT20 - Exhibit AT20.3, revision 06-01-11

Page 57 of 110 I~!! \\,;gj TVA I Sequoyah Nuclear Plant, Outfall 101 UV-treated May 08-15, 2012 7875 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification ofData Entry, Calculations, and Statistical Analyses Project Dumber. Environmental Testing Solutions, Inc Not for Com Hance Assessment, Internal Laborato C Coru:nl;ration (%) Replicllfe lDitlalllllmber of Final umber 0I1a..."" A _ Paa....ight (Ing) B '"' Pan +Larvae Un""....;gllt (mg) Welght'Surviving Meall...eillbtl CoetIIclent arvlUiatkm Welghtllnltialnlllaber MoaD.urvl.vai Me.... weight IlDltlai Coelficj.,ntof p.,,,,enf ",dqotioD from IlIrvae welght(1ng) '"'A_B nllmbe:r orlanae (mg) SnrviriDg number of (Meo.W<lRht,............... ofla...... (mg) (%) IInmbe:roflarvae variation (Moan wrigh! cotltrol (%) larvae (mg) ..........n......1W.) ("'" 1""lniljaln::orluno) A 10 (Q 13.77 19.68 5.91 0.591 0.591 Control B 10 (Q 13.56 20.03 6.47 0.647 0.586 9.9 0.647 100.0 0.586 Not applicable C (Q (Q 14.13 19.20 5.Q7 0.507 0.507 D 10 (Q 13.25 19.24 5.99 0.599 0.599 E 10 10 14.39 20.85 6.46 0.646 0.646 10.8% F 10 10 13.94 20.15 6.21 0.621 0.645 5.8 0.621 100.0 0.645 5.8 -10.1 G 10 10 13.89 20.05 6.16 0.616 0.616 H 10 10 13.94 20.92 6.98 0.698 0.698 I 10 10 14.12 19.65 5.53 0.553 0.553 21.6% J 10 10 13.54 19.76 6.22 0.622 0.624 8.2 0.622 100.0 0.624 8.2 ".S K (Q 10 12.70 19.29 6.59 0.659 0.659 L 10 10 14.06 20.69 6.63 0.663 0.663 M 10 (Q 13.93 19.58 5.65 0.565 0.565 43.2% N 10 (Q 13.52 19.61 6,09 0.609 0.604 4.7 0.609 100.0 0.604 4.7 -3.0 0 10 (Q 14.95 21.29 6.34 0.634 0.634 P 10 (Q 13.26 19.32 6.06 0.606 0.606 10 10 14.40 19.77 5.37 0.537 0.537 86.4% R 10 10 14.46 20.19 5.73 0.573 0.570 5.4 0.573 100.0 0.570 5.4 2.7 S 10 10 13.36 19.46 6.10 0.610 0.610 T 10 10 14.21 19.81 5.60 0.560 0.560 U 10 10 13.58 20.31 6.73 0.673 0.673 100% V 10 10 14.42 21.22 6.80 0.680 0.671 L8 0.680 100.0 0.671 1.8 -14.4 W 10 10 14.07 20.83 6.76 0.676 0.676 X 10 10 13.12 19.65 6.53 0.653 0.653 Y 10 (Q 13.71 19.68 5.97 0.597 0.597 100% Intake Z (Q 13.83 19.75 5.92 0.658 0.630 10.1 0.592 0.614 ".7 AA 10 10 15.29 20.90 5.61 0.561 0.561 97.5 10.2 BB 10 (Q 13.20 20.24 7.04 0.704 0.704 til*o Q"tfalll0l: Dunnett's MSD valne: PMSD: 0.0670 11.4 MSD"" PMSD"" Mmimum Significant Difference Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. In_ Dunnett's MSD valne: PMSD: 0.0829 14.1 Lower PMSD bound determined by USEPA (l Oth percentile) "" 12%. Upper PMSD bound detennined by USEPA (90th pefcentile) "" 30%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, ofPMSD datafrom EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001h). USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-0l-D04 and EPA-821-B*Ol-005. US Environmental Protection Agency, Cincinnati, OR. File: sqn101_050812data-uv.x1sx Entered by: J. s~~r Rev'lewed by: ~

Page 58 of 110 ~ETS ~ Environmental TestingSolution., Inc. TVA I Sequoyah Nuclear Plant, Outfall 101 UV-treated May 08-15, 2012 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Test ID: PpFRCR Sample ID: TVA I SON, Outfafl101 Lab 10: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP*Pimephales promelas Start Date: End Date: Sample Date: Comments: Conc-% 5/8/2012 5/15/2012 May 2012 UV-treated 1 2 3 D-Control 10.8 21.6 43.2 86.4 100 Intake 0.5910 0.6460 0.5530 0.5650 0.5370 0.6730 0.5970 0.6470 0.6210 0.6220 0.6090 0.5730 0.6800 0.5920 0.5070 0.6160 0.6590 0.6340 0.6100 0.6760 0.5610 0.5990 0.6980 0.6630 0.6060 0.5600 0.6530 0.7040 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSO Mea. N-Mean D-Control 0.5860 1.0000 0.5860 0.5070 0.6470 9.929

  • 0.6185 1.0000 10.8 0.6453 1.1011 0.6453 0.6160 0.6980 5.817

-2.130 2.410 0.0670 0.6185 1.0000 21.6 0.6243 1.0653 0.6243 0.5530 0.6630 8.163 * -1.375 2.410 0.0670 0.6185 1.0000 43.2 0.6035 1.0299 0.6035 0.5650 0.6340 4.734 * -0.629 2.410 0.0670 0.6147 0.9938 86.4 0.5700 0.9727 0.5700 0.5370 0.6100 5.358

  • 0.575 2.410 0.0670 0.6147 0.9938 100 0.6705 1.1442 0.6705 0.6530 0.6800 1.792 *

-3.038 2.410 0.0670 0.6147 0.9938 Intake 0.6135 1.0469 0.6135 0.5610 0.7040 10.171 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.9571 0.884 -0.4767 0.34009 Bartlett's Test indicates equal variances (p = 0.29) 6.13792 15.0863 Hypothesis Test (1-tail. 0.05) NOEC LOEC ChY TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.06704 0.1144 0.00565 0.00155 0.01863 5, 18 Treatments vs D-ControJ Linear Interpolation (200 Resampfes) 95% CL(Exp) Skew 1.0 0.9 0.8 0.7 O.S ~ 0.5 c 0.' g

0. 0.3

~ 0.2 0.1 0.0 -0.1 -0.2 -0.3 0 50 100 150 Dose % so >100 >100 >100 >100 >100 >100 >100 Point IC05 IC10 IC15 IC20 IC25 IC40 IC50 Dose-Response Plot 0.8...--------------------, 0.7 0.* los e C) 0.4 g "'" 0.3 0.2 1-tail,0.05Ievel of significance 0.1 Indopond,ol Rovl,whV ~II""~.K.,,,." ~' '"o File: sqnlOl_050812data-uv.x1sx Entered by: J. s~er Reviewed by:U

Page 59 of 110 eETS

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,,) o.*.'.....*...,..';.. '., ,~ EnvIronmental Testing Solutions, Inc. TVA / Sequoyah Nuclear Plant, Intake UV-treated May 08-15, 2012 Statistical Analyses Start Date: End Date: Sample Date: Comments: 5/8/2012 5/15/2012 May 2012 UV-treated Larval Fish Growth and Survival Test-7 Day Growth Test ID: PpFRCR Sample ID: TVA 1SQN, Intake Lab 10: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02*013 Test Species: PP-Pimephales promelas Conc-% D-Control 10.8 21.6 43.2 86.4 100 Intake 1 0.5910 0.6460 0.5530 0.5650 0.5370 0.6730 0.5970 2 0.6470 0.6210 0.6220 0.6090 0.5730 0.6800 0.5920 3 0.5070 0.6160 0,6590 0.6340 0.6100 0.6760 0.5610 4 0.5990 0,6980 0,6630 0.6060 0.5600 0.6530 0.7040 Transform: Untransformed Conc-% Mean N-Mean Mean Min Max CV"A. N D-Control 0.5860 1.0000 0.5860 0.5070 0.6470 9.929 4 10.8 0.6453 1.1011 0.6453 0.6160 0.6980 5.817 4 21.6 0.6243 1.0653 0.6243 0.5530 0.6630 8.163 4 43.2 0.6035 1.0299 0.6035 0.5650 0.8340 4.734 4 86.4 0.5700 0.9727 0.5700 0.5370 0.6100 5.358 4 100 0.6705 1.1442 0.6705 0.6530 0.6800 1.792 4 Intake 0.6135 1.0469 0.6135 0.5610 0.7040 10.171 4 i-Tailed t-Stat Critical MSD -0.645 1.943 0.0829 Critical Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.01) F-Test indicates egual variances (p = 0.91) Hypothesis Test /1*tail, 0.05) Homoscedastic t Test indicates no significant differences Treatments VB D-Control Statistic 0.9723 1.15016 MSDu MSDp 0.08289 0.14146 0.749 47.4683 MSB 0.00151 MSE 0.00364 Skew Kurt 0.34802 -0.3978 F*Prob df 0.543 1,6 Dose-Response Plot 0.8,.-------------------, 0.7 0.6 lO.6 e Cl 0.' ~ C 03... 0.2 0.1 1-tail, 0.05 level of significance 1"~.F>I'"d,nt R","owtl\\' KoII*I~vc:..n 0g 0 .Ii 0 N 0 c ~ ~ 0() cI File: sqnlOl_050812data-uv.xlsx Entered by: J. Sumner Reviewed by: -----l:f-

Page 60 of 110 Page 5 of6 SOP AT20 - Exhibit AT20.3, revision 06-01-11 21.6% 43.2% 10.8% 86.4% 100%< 100% Intake CONTROL UV-treated Species: Pimephales promelas Date: ()$..08*11..- Client: TVA I Seguoyah Nuclear Plant. Out1a1l101. UV-treated --"'=......-'--=-- Dai Chemist Concentration Analyst 1-~~~r-::=:---I-:~:r--i"""":~:-""~~-r--:----1 ~---~---- <f L.,:* I I, I I I f j ) J I ~

Page 61 of 110

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!D ~ E-"-'m-,m-"-"-'T'-'"-',-"-,,,-,,,-,,,-". Species: Pimeohales oromelas Client: TVA / Se uo ah Nnclear Plant Outfall 101 UV-treated Date:

  • 01-1"1-Page 6 of6 Da SOP AT20 - Exhibit ATZO.3, revision 06-01-11 21.6%

10.8% 43.2% 100% 86.4% Concen-Parameter tration 100% Intake CONTROL I--':''~~__-II-'=:L-_ UV-treated Analyst....-.,.......;i--.--+,.",.--ii-~--f.....~~-;;..-.----+-~---i;...-._--1 1---...,..---""";"- I I, I, ~ I I I I I I I I I I

Page 62 of 110 1"1!i '~i.!f TVA / Sequoyah Nuclear Plant, Outfall 101-UV-treated May 08-15, 2012 eElS @I ',',','e"" \\,'~,'~ o I') Environmental Testing Solutions, Inc. Pimephales promelas Chronic Whole Efflnent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project Dumber: 7875 Concentration IParameter Day 0 I Dav! I Dav2 1-Dav3 1-Dav4 I DavS I Dav6 Initial I Final I Initial I Final I Initial I Final I Initial I Final I Initial I Final I Initial I Final I Initial I Final oR (SUI I 7.881 '-----r.911 7.981 7.811 7.981 7.84 8.071 7.51 7.8 7.8 """"~'" ~~. ~~,

      • .""""~;""

~hh - "'"'=''''''' Control tnH (SUI I 7.881 7.841 7.981 7.821 7.981 7.851 7.981 7.671 7.941 7.601 7.911 7.631 8.091 7.50 6.8 10.8% TemDerature ('C) I 25.01 24.81 25.01 24.91 24.81 24.51 24.81 24.41 25.01 24.51 24.81 24.61 25.01 24.5 IDH (SUI - I 7.891 7.901 7.991 7.821 7,981 7.851 7.981 7.681 7.931 7.611 7.891 7.611 8.101 7.50' 21.6% 6.8 ,Temnerature ("C\\ I 25.01 24.81 25.01 24.9\\ 24.81 24.51 24.81 24.71 25.01 24.71 24.81 24.61 25.01 24.5 oR (SUI I 7.891 7.891 7.991 7.831 7.971 7.841 7.991 7,681 7.881-7.63T 7.861 1:6018.i1l 7.50 43.2% OOm~ 68 Conductivity(~ho~cm) Temperature("C) I 25.01 24.51 25.11 24.61 24.81 24.61 24.81 24.81 25.01 24.71 24.81 24.61 25.01 24.5 oR (SUI I 7.901 7.901 8.001 7.821 7.941 7.881 8.001 7.681 7.811 7.731 7.791 7.651 8.131 7.50 86.4% DO m 6.7 Conductivity (umbos/em) Temperature ('C) I 25.01 24.71 25.11 24.61 24.81 24.61 24.91 24.61 25.01 24.81 24.81 24.91 25.01 24.6 oR (SUI I 7.921 7.911 8.021 7.841 7.941 7.891 8.021 7.78 8]51 7.50 7.81 8.1 8.0 7.9 ~~_J .On' "'~I_ 'An ~:.~.>iiP!l:lt DO m Conductivi mbos/em 10'::1' ': ~.~ Alkalinity (mglL CaC03) Hardness (mglL CaCO]) Total Residual Chlorine m Temoerature (0C) 100% IDH (SUI I 7.941 7.95 '----s.i61 7.52 7.9 .n./ "",,,';;..- k*'.io.E .n.1, "",_"'~ 1n.. ~.,~. .n.,> 100% Intake File: sqn101_050812chem-UV.xls Entered by: C. Jj\\on Reviewed by:

Page 63 of 110 I Page \\"1. Page _I_*of_'\\.._ Total Residual Chlorine (Orion Electrodl;l MethOd, Orion 97-70) Matrix: Water, RL = 0.10 mgIL Meter: Accumet Model AR25 pH/Ion Meter I I I I I iET " ~ EnvlronmentalTe5ting Solutions-Inc. Date a~;~:~ I==;~~~:.():a=.:,'l.==== Calibration: 1.00 m Iodide reagent: !.....":LN"""""'<t-9o:.;,,I.,,!QL.- Acid reagent:. ~~" Slo e I L b a oratory contra stan ar : Reference standard Troe value (TV) Measured value (MV) % RS-MV lTV x 100 number (mglL) (mgIL) (acceptable range = 90 to 110%) ~I\\l!'~louL 0.50 0.<2,9-10"'" To °1. I I LV.OOI LO.OOI LO.OO .t::: D* 00 .cO.oot G Reviewed by I""'3rl' Date reviewed [$-o-:-;;.t-.,"L.:--- (,..1 %RPD = (IS - D) 1[(S+D)/2]} x 100 (acceptable range = :1:10%) Sample characteristics Sample characteristics SampleID ed in excess ofEPA recommended holding time (15 minutes) unless otherwisenoted. I L b a oratorv contro stan ar, : Reference standard True value (TV) Measured value (MV) % RS-MY ITVx 100 number (mgIL) (mglL) (acceptable range = 90 to 110%) -:t.N"~ID,A 0.50 O. 'SII, IO'2.,:l.,.-J. 1-"-'~!o!<='=F-"C.ll"",u..':""--::- __ d Duplicate I I I I I I I I I I I SOP C8 -Exhibit C8.1, revision 06-01-11

Page 64 of 110 Page ')..0 Page ----'!:..- of~ Calibration: ~ 0.10 iiigil:: I 1 gg roga I Slope '* '~.\\'V Note: For samples with a residual chlorine of > 1.0 mgIL, the calibration range must be adjusted to bracket the chlorine levels ofthe samples. Iodide reagent: I-""*a.'k.,. I Acid reagent: I I I I I I

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~~ -~ EnvironmentalTe5ting Solution5, Inc. Analyst I_-I!"'~"- _ Date analyzed _-'9"':"'.O=I'>"".I:L"'--__ Total Residual Chlorine (Orion Electrodf MethOd, Orion 97-70) Matrix: Water, RL = 0.10 mgIL Meter: Accwnet Model AR25 pH/Ion Meter z.. Lo.o Co.o ,( 0.001 L. r %RPD = (IS - D) 1[(S+D)/2]) x 100 (acceptable range = r 10%) (. t..... Sample characteristics Sample characteristics SampleID L b Du licate sam Ie reCISIOn: Sample Sample ID number a oratorv controlstandard: Reference True value (TV) Measured value (MV) %RS MV lTV x 100 number (acceptable range 90 to 110%) 0.50 0I!..11b.\\l-IllY I I I I I I I I Note: All samples were analyzed in excess ofEPA recommended holding time (15 minutes) unless otherwise noted. It d I b La oratorv contro stan aT : Reference standard True value (TV) Measured value (MV) %RS-MV/TVxlOO number (mg/L) (mglL) (acceptable range = 90 to 110%) .,.-r* *_"f 0.50 0.#'8 &'1 r../* Reviewed by I--,a(~--::cc-::__ Dale reviewed ~--"O",$:...*",a",ll*-,I-,"L=-_ SOP C8 -Exhibit C8.1, revision 06-01-11

Page 65 of 110 l!l J TS ,~ EnvironmentalTestlng SolutiDns, Inc. Page "Z.S Page_I_6f~ Iodi~e reagent: I~~",,-,'l:.,,-~-,,,,,-- ACId reagent: [£N,._.!!a....:l~""'- Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, Rl;~ 0.10 mgIL Date a:;;'~ l-os-=~:O'1"'6'--'\\1------11 Meter: Accumet Model AR25 pH/Ion Meter Calibration: 1.00 m IL Sio e Note: For samples with a residual chlorine of > 1.0 mgIL, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratorv controlstandard: Reference standard True value (TV) Measured value (MV) % RS-MV lTV x 100 number (mglL) (mglL) (acceptable range = 90 to 110%) n....S ~ 0.50 O,SIO IQZ.~% ceUle.. Duplicate Sample characteristics Residual chlorine m IL) : %RPD = (IS - D) 1[(S+D)/2]) x 100 (acceptable ra e =:1: 10%) <'0.000 rtC7 (0.00$'(7 Sample characteristics Sample Sample ill number Sam Ie measurements: Note: All samples were analyzed in excess ofEPA recommended holding time (15 minutes) unless otherwise noted. Laboratorvconuolgandard: number True value (TV) Measured value (MY) (mglL) %RS MY/TVxlOO (acceptable range 90 to 110%) 0.50 Reviewed by If,~~,""-,,,--- Date reviewed 0510 1'1.. SOP C8 - Exhibit C8.1, revision 06-01-11

Page 66 of 110 I I I I Page z.J: Page ~ of_'&._ iET ,~ Env/ronmentalTllstlng Solutions, Inc. Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL ~ 0.10 mgIL Meter: Accumet Model AR25 pH/Ion Meter Analyst 1f---li,,",\\1\\~ Iodi~e reagent: I ----- ] Date analyzed _.!o. rt-ACId reagent:. """.r-ao"',<l.h Calibration: Note: For samples with a residual chlorine of > 1.0 mgIL, the calibration range must be adjusted to bracket the chlorine levels ofthe samples. I 1.00 m IL SI. e ~ OS',"'.... d d I L b a oratory contro stan ar : Reference standard True value (TV) Measured value (MV) % RS= MV ITVx 100 number (mgIL) (mglL) (acceptable range = 90 to 110%)

Ilo)SS 101 0.50
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'i'Z..O,," I I (0.00'2.1! c..O.OOIO'2: (,0. OOC., Z. %RPD = {(S. D) 1[(S+D)/2]} x 100 (acceptable range =:1:-10%) Sample characteristics 1\\. \\.I'j doJ.:I....,..~.tcs Sample characteristics Sample ID Rea ent Blank Duplicate I I I I I I I Note: All samples were analyzed in excess ofEPA recommended holding time (15 minutes) unless otherwise noted. Reviewed byI~ Dale reviewed -;M;"'~*I-::O"'.71-z...:------1 d d I L b a orutorv contro stan ar : Reference standard True value (TV) Measured value (MY) % RS-MV ITVx 100 number (mgIL) (mgIL) (acceptable range = 90 to 110%) -r..,IC' I"' 0.50

o. SIC

'03. Yo. I I I I SOP C8 - Exhibit C8.1, revision 06-01-11

Page 67 of 110 Note: For samples with a residual chlorine of > 1.0 mgIL, the calibration range must be adjusted to bracket the chlorine levels ofthe samples. Iodide reagent:I'~f-. 6)\\0 Acid reagent: I fl.6Tl Sio e 0.10 m IL Total Residual Chlorine (Orion Electrode Metholl, Orion 97-70) Matrix: Water, RL =0.10 mgIL Meter: Accumet Model AR25 pH/Ion Meter It d I L b Page.30 Page~6f~ Analyst 1---=,-;t1t!==:;)AJ~_--1 Date analyzed

'=06~-"*I-=1-_.:....:'"l.::--.-J Calibration:

eEl t> ~,d "7': ~ EnvlronmentalTestlng Solutlons,lnc. a oratorv contro stan aT : Reference standard True value (TV) Measured value (MV) % RS-MY lTV x 100 number (mglL) (mglL) (acceptable range = 90 to 110%) IJ\\15s 10iJlJ 0.50 n.6z.e IOS.6 7~ I I I I I I I Note: All samples were analyzed in excess ofEPA recommended holding time (15 minutes) un eSs otherwise noted. Residual chlorine mIL .oo~ UJ. OQ:XlO DO %RPD ={(S - D) 1[(S+D)/2]} x 100 (acceptable range =:1: 10%) Sample characteristics Da licate sam Ie recision: SOP C8 - Exhibit C8.l, revision 06-0l-Il Reviewed by r--31"...,,--::::-__ Date reviewed ~'"1-' '1.... Sample Sample ID number Laboratory con Reference standard True value (TV) Measurea va v % RS *.MV ITVx 100 number (mgIL) (mgIL) (accept:Wl't'kange 90 to 110%) 0.50 OSZIt. I I I I I I I I I

Page 68 of 110 Note: For samples with a residual chlorine of > l.0 mgIL. the calibration range must be adjusted to bracket the chlorine levels ofthe samples. Page 0) Page~of~ Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, RL =0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent:E OS I~\\t, Acid reagent:. u SID e 1.00 m IL Analyst C}iiu;-..> Date analyzed D$1".I"'Z.=,yz'---- Calibration: iETS

~ EnvironmentalTesting Solutions, Inc:.

%RPD = (IS - D) 1[(S+D)/2]} x 100 (acceptable range =::!: 10%) Sample characteristics SLO.OOIZZ Sample characteristics Residual chlorine m IV 101 *iJT Sample ill d d I -rJIt. WBiJ 10) 1\\ IIJT L b a oratoro contro stan aT : Reference standard Troe value (TV) Measured value (MV) %RS-MV/TVxl00 number (mglL) (mgIL) (acceptable range = 90 to 110%) ll\\I"S< IOlo" 0.50 fl.IUL ('\\1.'2. 7~ I I I I I I J:i -, I I I Note: All samples were analyzed in excess ofEPA recommended holding time (15 minutes) unless otherw-ise noted. I I Laboratorv controlstandard: Reference standard True value (TV) Measured value (MV) %RS-MV/TVxl00 number (mgIL) (mglL) (acceptable range = 90 to 110%) h.\\~~fOllJ" 0.50 n...fp,q '\\1.H. Reviewed by c:::::M-,-:;---,-=-_-i Date reviewed ~. It.- !L I SOP C8 - Exhibit C8.1, revision 06-01-11

Page 69 of 110 Page \\ of----'-__ Analyst 1-=.:r45=--- Date analyzed OS*.os. 12. Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCO,/L Titrate samples to pH= 4.5 S.U. Time initiated 1_...:1"'°""- Time completed _--',"'.::l!..OSL-Du licate sam Ie recision: Sample Alkalinity %RPD= Sample SampleID volume Begin End Total Multiplier (mg CaCO,IL) (IS - D) /[(S+D)/2]) x 100 number ml ml ml ml (acceptable range = +/-lO%) 06.o~.1l. 5 W 'Itc 'I:'l.'l 10.'1-s 3&:1 \\0<'> 3.3 \\ Duplicate (B) I D ~*1.1 '1:'.2 3*3 3'i Titrant normalitv and multiDlier determination: pH of Normality Normality (N) of H2SO4 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na2CO, x 0.05)/E =(N x 50000)/100 ml sample water reference standard ml ml ml =0.25/E =Nx500 = 4.5 S.U. number number (E) (acceptable *range = 0.0180 - 0.0220) 'I.'

m"'it" 'l:WIS 1016 0.0

~l..o ,'t.o C>.0709 IO.'t a oratolV contro stan ar : Reference standard True value Sample Alkalinity (MY) %RS-MY/TVxIOO number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range (mg CaCO,IL) (ml) ml ml ml = 90 to 110%)

t'l\\IU 10.17 100 100

,~.o ~I., 1.' 10.,+ \\00 ,,,o.o~ Ii Ii I I II Date reviewed: 100.0":\\42.. 31.1 SOP C6 - Exhibit C6.1. revision 06-01-11 RevIewed by: l M. 'k s afflX SPI e recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO,IL) (rna CaCO,IL) (mil ml ml ~Mml rllss 1037 So \\00 Z'l." ~ -a.a 10.&1 i3 Sample alkaliuity (B) Measured spike value (MV) % R-MV / SVx 100 (mg CaCO,IL) MV=A-B (acceptable range (rna CaCO,IL) = 75 to 125%) 3~ 'is.Ot: amme measurements: Sample volume Begin Eud Total Alkaliuity Samole Dumber SamnleID (ml\\ ml ml ml Multiplier (rna CaCO,IL) 0'5-0\\.\\"2.- tn\\o\\s/"v 100 3'Z.' 3'.0 '.1 10.'1 '3 oS*O'b.,w, '3....(> ..5.\\ '.1 a Q5.0~*1"~ 5*3 \\I.Ii '.1 '3 O'-\\'W.rt-11.'1 l7.S '-I '3 Q3O"lCi l.k.t.wv.. .sc, 'l.S.Cl

)0.'

S.? ('Z~ I'LO ~ "'.(>&.,... IJ II II II Ii

Page 70 of 110 Page _'--------'of fl Page _3",-,--'_ Analyst I--~='------l Date analyzed D5'\\Z'I~ Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCO,1L Titrate samples to pH =4.5 S.U. Time initiated Ir--=:..""""c.'----- Time completed L_-'-'lt....-'qL-recision: Sample Alkalinity %RPD= Sample SampleID volume Begin End Total M,ultiplier (mg CaCO,/L) <<8 - D) 1[(8+D)I2]J x 100 number ml ml ml ml (acceptable range =:1: 10%) ex::.,IC.IL 5SvJ 100 U( 25,1 3.3 ,J; Duplicate (B) U.l ta.'I 3.' Titrant normality and multiplier determination: pH of Normality Normality (N) of H,S04 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na,CO, x 0.05)/E = (N x 50000)1 100 ml sample water reference standard ml ml ml =0.25/E =Nx500 =4.5 S.U. number number (E) (acceptable range = 0.0180 - 0.0220) S.\\ %IolR,\\t" X~S 'ott 0,1 It.Z I'z.1 0.01.07 \\O.~ a oratory contro stan af', : Reference standard True value Sample Alkalinity (MV) % RS=MV ITVx 100 number (TV) volume Begin End Total Multiplier (mg CaCO,IL) {acceptable range (mg CaCO,/L) (ml) ml ml ml ~ 90 to 110%)

l:lIISS 1037 100 100 I'Z:Z lUI 'I.'

to.'! \\00 100,0% I I I I I I Date reViewed: I US*I'*l"" SOP C6 - Exhibit C6_1. revision 06-0 I-II ReViewed by: S l Matrix slJike recoverv: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO,/L) (m. CaCO,/L1 (mil ml ml ml

tMSS 10J?

So lot> as. I '31:& t.1 'O.~ 8"1 Sample alkalinity (B) Measured spike value (MV) % R-MV ISVx 100 (mg CaCO,/L) MV=A-B (acceptable range (m. CaCO,/L) =75 to 125%) 3"\\ 50 ,0C!.0% amme measurements: Sample volume Begin Eud Total Alkalinity Sample uumher SampleID -(mil ml ml ml Multiplier (m. CaCOJ/L) 0'5.Cf'j,I 'l.1b ImH.stN 100 lOU 1"., '.Z 10." i 06, 1I,jl-A 3'l.Q 'ls,s C.l '3 0'6. i).a b 0.0 '.0 t:.Cl 'z 1[}';-O'1'12~' \\l.' O'S. otI-t2ft LJ V Il.' IS.~ '.1 ~01?'?'Z2 5b ".'1 'ZloS "103 (2.) 6'1 " 7.\\.111 Cbln; (L, tOo 'Z'&,.5 U.' '.1 '3 1 c.\\.u Omt..- U., 13".7 '.1 'J ...v PPE\\c:.o(l)l(.. ,I.- J"\\.7 '10" ':1. \\ IJ I I I I I

Page 71 of 110 I Ii>.., o-',,-'ro-,m-"-"-'T,-stj-,,-S'-'"'-'"--' Page .It Page ~ of-",8__ I I Analyst jf-----':NI= Date analyzed L....:O:>S"-."'I~"'.:.!.I1:==---__ Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCO,1L Titrate samples to pH=4.5 S.U. Time initiated I=:-----.. I Time completed ...........;,qs 05011../1. I I Titrant normality and multiplier determination: pH of Normality Normality (N) of H2SO4 pH Factor or Multiplier d Titrant check Begin End Total (5 ml Na2CO, x 0.05)/E (N x 50000)/ 100 ml sample water re standard ml ml ml 0.25/E Nx500 4.5S.U. number num (E) (acceptable range 0.0180 - 0.0220) 0I'!t.,t. d d I recision: Sample Alkalinity %RPD= Sample Sample ID volume Begin End Total Multiplier (mg CaCO,lLl {IS - Dl 1[(S+D)I2]J x 100 number mil ml ml inl (acceptable range = +/- 10%) -OO*\\t.A Mil-vV I IS.' '.0 s ,~ (I 100 ~ Duplicate (B) .L 0 3 IS.' ~1.7 '.1 L b a oratory contro stan ar : Reference standard True value Sample Alkalinity (MV) % RS-MV ITVx 100 number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range (mg CaCO,IL) (mil ml ml ml =90 to HO%)

P\\5$ 103'7 100 100 0.0 "I.e

'f., 10.'/ 100 100.0" I I I I I Matrix slJike recovery: Reference standard Spike value number (SV) (m. CaCO,tL) Sample volume imll Begin End ml ml Total ml Multiplier Spike alkalinity (A) (mg CaCO,lLl Sample alkalinity (8) (mg CaCO,IL) 110 %R-MV/SVxl00 (acceptable range = 75 to 125%) 10."! Measured spike value (MY) MV=A-B (mg CaCO,lL1 IOQ So I I I I I I I Sample measurements: Sample volume Begin End Total Alkalinity Sample number Samplem . (mil ml ml ml Multiplier (m. CaCO,IL) Os.* <T!:r1"1.& m~,<, \\)\\1 2-100 '2.C."I '2.5 '.1 IO.~ "3 o;,,-lr\\- 1"1- ~ 31..$ 31.7 '*l -'<+ (6-1:1'\\- ILA 4: 31."7 "lit'! '.'t 'If b~-I\\' I""Z.. ~ II 5" 0.0 '.1 '_I C3 I "1.0!>'1.1.0 \\ A'6/M """tL- '.1 IU.o 4.11 11:16 \\ MU2-01Y\\tt. IC.o -zu I.' 100 mMc.-omrv U **. n.1 "1.3 "'t"7 1/ MfbUl-.J){Y\\/V 135.1 "l'U. '1.1 'IS 06-\\1.11... SCLJ,:.!-&N C>,() f.e II ~., ReVIewed by: 6\\ Date reviewed: I ClS*IJ.*n.... SOP C6 - Exhibit C6.1. revision 06-0I-II

Page 72 of 110 Page .13 Page.s of---'l'--_ Ana~stl__~~~Q5=- Date analyzed OS.,,&,"- Alkalinity (8M 2320 B) Matrix: Water, RL = 1.0 mg CaC03/L Titrate samples to pH =4.5 S.U. Time initiatedI=-:..-......... I Time completed ~ "'*"*rr Titrant normality and multiplier determination: -nU.nf' Normality Normality (N) of H2SO4 pH Factor or Multiplier a Titrant-Deionized .L .1. BeSlin End Total = (5 ml Na2C03 x 0.05)/E = (N x 50000)1 100 ml sample water reference standard ml ml 1..1

.. = 0.25/E

=,Nx500 4.5S.U. number number (E), (acceptable riiig~ -e.""' - RR220) ~OI'R*rt. d d I L b a oratory contro stan aT : Reference standard True value Sample Alkalinity (MV) % RS= MY lTV x 100 number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range (mg CaC03/L) (ml) ml ml ml =90 to 110%) 'J:NSS 10'3' 100 100 I,'.,.S

    • 7 10."1

'01 \\Ol.Ot. %RPD= {(S - D) /[(S+D)/2]) x 100 (acceptable range = +/- 10%) Alkalinity (mg CaC03/L) D s 'II., 16.0 \\06 Sample volume Begin End Total Multiplier ml ml' ml ml Sample ill recision: Duplicate (B) ~.. Sample number Matrix spike recovery: Reference standard Spike value number (SV) (mg CaC03/L) Sample volume Begin End Total (ml) ml ml ml Multiplier Spike alkalinity (A) (mg CaCOJIL) ISo S l Sample alkalinity (B) Measured spike value (MV) % R=MV ISVx 100 (mg CaC03/L) MV=A-B (acceptable range (mg CaC03/L) =75 to 125%) 1<>0 'So 'Oo.O~ amp4 emeasurements: Sample volume Begin End Total Alkalinity Sample number Sample ill (ml) ml ml ml Multiplier (mg CaCOJIL) OS*~*a.6 SGU..:t 6lA.l l4:fO 0&0 CI.' t' Io.~ l04 06dD.t'L l l*.3 tf.... 4fC 06..08*1'z, J" \\JI '**3 . 11.1

  • . f

'140 \\'"l.l\\<"'tC"1 n \\ 1VASGi'l lal \\ 6b ~1., a.ct 3** tD "1CI \\1..~.1.S - z.. 32.9

SC.I 3.ct

~( "" ~__ ~~.""*~T~j,"Z.O~,\\.~ 3 3C** 'iO.-' 'I.! 7. \\ 4()~U111 tr1- '(~Asf~,~, . ~o.,* '('1.1 \\.1 77 \\~. ~ot;.1.'- 2-t+'t3 "'t.f 3.' 71 \\ L..be;,\\*"L.\\ ,~ .3 .,~ 0.0 1.' 3." " ~v Reviewed by: Date reviewed: I OS* It I \\-z... SOP C6 - Exhibit C6.1. revision 06-01-11

Page 73 of 110 Titrant normalitv and multiplier determination: -.u.!!. of Normality Normality (N) ofH2SO, pH Factor or Multiplier DeioRlze rant check Begin Eud Total (5 ml Na,CO, x 0,05)/E (N x 50000)/100 ml sample water reference ml ml ml 0.25/E Nx500 4.5S.U. number number lEI (acceptable range 0.0180 - 0.0220) ~ CS1'2..l"l.. Page 3'1 Page f.! of 8' Time initiated ~ I Time completed ~ ~ os*lao.,&. Titrate samples to pH = 4.5 S.U. Alkalinity (SM 2320 B) Matrix; Water, RL = LO mg CaCO,1L Ana~st I~~~CWL Date analyzed,---=o..,S,-.t.,:1:,-'..,tL......__

    • e

ill ,~ =,,,='**='m-..-"-'T.-"'-.,-S.-,,,....:,**-,,,-,,, Date reviewed: I Cl!)'1'2'rz.... I SOP C6 - Exhibit C6. I. revision 06-0I-I I ReViewed by: d d I b Du IIcate sam Ie recision: Sample Alkalinity °.loRPD = Sample SamplelD volume Begin End Total Multiplier (mg CaCO,IL) {(S - D) 1[(S+D)/2]} x 100 number ml ml ml ml (acceptable range = +/- 10%) \\"2-50

J,8 tl)

J, .7.Z ?o.". 3.7 j, 77 '2.~~ M Ix 'k S l La oratory contro stan ar : Reference standard True value Sample Alkalinity (MV) % RS=MV ITVx 100 number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range (mg CaCO,IL) Imll ml ml mI = 90 to 110%) I"NSS' 1~"7 100 100 3.8 11.'1 1.' 10.'" \\00 Ic.O.o% atr SOl e recoverv: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO,IL) 1m. CaCO,IL) 1m)) ml ml ml 1l61~lt

PJSS l~'H IDa 8)

.,. a. ~& '.of (1) 10.<\\ ~leo Sample alkalinity (B) Measured spike value (MY) % R-MV ISVx 100 (mg CaCO,IL) MV=A-B (acceptable range 1m. CaCO,/L) -75 to 125%) ..,1 ",,1.- --'i3"i' to? ~\\l~*r'.o':' 1030/. amme measurements: Sample volume Begin End Total Alkalinity Samole number SamnlelD 1m)) ml ml ml Multinlier 1m. CaCO,IL) \\ "'L(f.,(j'\\.1.S -rirf\\'.::G~ I 0\\ 1)\\1 2-50 'ZS-' 7.1.'1 '.S... 'i\\ \\, "1.\\ 71 \\'1..(~1\\. "to -It 3 7.1.1.\\ n.t. 3011' 7'"

n. ao;.cr1. en..

101 J.r '1'11'A::£:INI""W \\ n,z. '3'.0 7<¥ \\ "2-OS,Il'l.'2.1. I 'Z J?Co <to.? 3.7 77 1<.~11.'2.\\ J,

3 "iO.7 Oo/ll.S "3.&'

7q '"2.Q'i>ln.tA. "iVA wl,t.,} 10\\ \\ 0.0 '1.\\ <.j.t >gs \\"2. OSO'\\.2.1 I ~ ...\\ U 4.2-fl7 \\ '2.0<,. \\ \\. "1.1... ~ 3 1I.:i 11..&1 '1.\\ 85 \\"'l.. oS (fl.OY '1"JA W~rJ \\OII>JT I It.If .,. J

'l*ot V

111 I I I 11 I'" II I, I Ii I 'i i I r I II, II I,

Page 74 of 110 I I ~ 10 ,~ E-"-iro-,m-"-..-'T.-"-,.-,,-,,,-,,,-,,-", Page J$ Page'S 0[----<6'--_ I I Analyst I ~ Date analyzed o.s.I'Z"I~ Alkalinity (8M 2320 B) Matrix: Water, RL ~ 1,0 mg CaCO,/L Titrate samples to pH =4.5 S.U. Time initiated I:::=---.... I Time completed ~ o'*IZ,*a. I II Titrant normalitv and multiplier determination: D~' d Normality Normality (N) of H,S04 pH Factor or Multiplier elODize Titran.. ~" ck Begin End Total (5 ml Na,CO, x 0.05)!E (N x 50000)/100 ml sample water reference standara ml ml 0.25/E Nx500 4.5S.U. number number facceotable range 0.Ot80 ~ 0.0220) .:ItS <IS*/Z*tto d d I L b a oratory contra stan ar : Reference standard True value Sample Alkalinity (MV) %RS=MV/TVxIOO number (TV) volume Begin End Total Multiplier (mg CaCO,/L) (acceptable range (mg CaCO,/L) (mil ml ml ml = 90 to 110%) ~s~, 100 100 ".3 1:'.0 '1.7 10.'/ 101 10/.oK IJ II wJ recision: Sample Alkalinity %RPD= Sample ID volume Begin End Total Multiplier (mg CaCO,/L) {IS ~ Dl /[(S+D)I211 x 100 mil ml ml ml (acceptable range =:l: 10%) 2- -Z':.O 'Z..**Of 11) S 81 \\ 3." .l.. .It D 1.'I."I '33.'I 'I.e 13 'Vi~ Ii ~I I Matrix sDike recovery: Reference standard Spike value number (SV) 1m. CaCO,tL) 100 Sample volume Imll Begin mI End ml Total ml Multiplier Spike alkalinity (A) (mg CaCO,/Ll 1'0 Sample volume Begin End Total Alkaliuity Sample number , Sample ID ' Imll ml ml ml Multinlier 1m. CaCO,/L1 \\ 'l. os.1\\.~ 1\\1A l,\\JlW 1\\)1 INn, 50 38.' 11.1'2.' ~.o l">> 16."1 ,<<3 1"l.D'i>tn OS'* ';-v'AWIQ,v II'?>, "Zo., I c.".Q cu. '47 \\'l. o!.O"\\."Z.C\\ I Z-0.0 '1.3 't.3 f.Cr I "Z.OiOl \\. '2.i.I .v 3 1.f.'3 ~... ct.1 is \\ "l.0<;,l!'"\\. til::. -rV'A-W~<U II~I~ I 1I.~ I"Z.., 7.'" 77 n (j';,O"\\.~ I

i!-

\\'2..1 15.'1 3.9 "7'1 \\"l.O~\\'.1.' V 3 ,I; 1$, " 1...1 '1.0 n \\., '''''".m r:Art.ITl1,llt I 100 ~1.S -a1.l{ 0." 'f.t1

n. (lS. 10. a1

~ z. 1,,- 1.'t." c:u. -z,9 oz." SOP C6 - Exhibit C6, I. revision 06-01-1 I I I I i iI I I I \\ Sample alkalinity (B) (mg CaCO,/L) Sample measurements: Measured spike value (MY) MV=A~B 1m. CaCO,/L) 17 ReVIewed by: % R-MY /SVx 100 (acceptable range = 75 to 125%) '17.0% Date revIewed: I ll&*,Z*!"L.

Page 75 of 110 Titrant normalitv and multiolier determination: Il1l.2!. Normality Normality (N) of H,S04 pH Factor or Multiplier Deionized check Begin End Total (5 ml Na,CO, x 0.05)/E (N x 50000)/100 ml sample water reference sian ml mi ml 0.25/E Nx500 4.5S.U. number number E) (acceptable range 0.0180*0.0220) -C.f.ll.*~ Page 3C Page 0[---"'---_ Time initiated I............... I Time completed. ~ ...*'1*.\\ Titrate samples to pH =4.5 S.U. Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCO,/L Analyst I-......:n.s"""--- Date analyzed OS.'Z.,t '!'I ~ E="='W='W=-"-""',T'="--C:-SO,c-'uti==-',,-". I I I I Ii Ii I Ii Ii Laboratorv control standard: Reference standard True value Sample Alkalinity (MY) %RS-MV/TVxIOO number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range (mg CaCO,IL) (ml) ml ml ml

90 to 110%)
r1'lo6 16.,,-7 100 100 35.'t

'1'1.'1 '1.5 ,e."f "1"1 '1'.0% Sample Alkalinity %RPD= Sample volume Begin End Total Multiplier (mg CaCO,IL) (S - D) ![(S+D)i2]} x 100 number ml) ml ml ml (acceptable range =::1: 10%) Z.$' z.i 10." s i.'1 0.<0 ~ D ~.9 5.' Z.~ ~"I SOP C6 - Exhibit C6.1. revision 06-01-11 Matrix soike recoverv: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO,iL) (m. CaCO,IL) (mil ml ml ml

l'Mli,S 10"57 Sr; 160

'Z.' 10.3 7.5 10.,,/ 7' Sample alkalinity (B) Measured spike value (MV) % R-MV 1SVx 100 (mg CaCO,IL) MV=A-B (acceptable range (mg CaCO,IL) = 75 to 125%) oz." "i.l>,", Samole measurements: Sample volume Begin End Total Alkalinity Samnle number Samole ID . (mil ml ml ml Multiplier (m. CaCO,IL) I ~ ~.. "-". I f"'\\

5

.L,('1\\".. I lOa 10.3 ISot 10.1./ Sf n.~ICl.O'\\ I z.. LS.l. ~O.() 'i,t So 1'2.~\\".08- .t, .3 ,I-z...o lu./ S.l 53 \\ 7 h"-" II. l\\"" C;:lU(.l.l. \\ )l,<; t,s:, Z'f.:t ~., ~) 170 \\'2,1"'-' rl. It%.. z.. l.1.l: 3'1.$ 5:3 -z.z.o ,") ~\\'1.en. 3 I.- l... S 31.0 Cf.S "0 '1.cSoa.o \\ ~o~ I 100 '3'1.0 "\\"'., 3.( 37 1<'OS'.IO.0 I \\ z. n.'* lK1 to.. CiS \\7..~ 12,. CJ \\ .J/ ..3 ,/ c...,... '.4 V chr..~ (pi-Date reviewed: I Cl S *,1.-I"L ReVlCwed by: I I

Page 76 of 110 Page irN"" I 1 1 l'l <i1 IJfJ ~ ="=Vlro=,m=-'-:--='T'-'--"-:C"-:-,,,=,,,=-,,:--", Analyst It--....,;r;"'~"--- Date analyzed L_o=~""-,Ic:::Z....:'I-,"t,--__ Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaC031L Titrate samples to pH =4.5 S.U. Page --,?~_.of 'i/ Time initiatedE I Time completed ~*I'7*'l: I Titrant normalitv and multiolier determination: ..f..H of ~, Normality Normality (N) of H,S04 pH Factor or Multiplier DelO t check Begin End Total (5 ml Na,C03 x 0.05)/E (N x 50000)/100 ml.ample water reference ml ml ml 0.25/E Nx500 4.5S.U. number number iEl (acceptable range 0.0180 - 0.0220)

1"7-s.:a oS*I'l..

11 I, Ii IJ Laboratorv control standard: Reference standard True value Sample Alkalinity (MY) % RS-MV /TVx 100 number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range (mg CaCO,IL) (ml) ml ml ml =90tollO%) ~S 103, 100 100 '.'i 'C.O,., 10.1.1 10C> 10O.O'i'd Sample Alkalinity %RPD= volume Begin End Tolal Multiplier (mg CaCO,IL) {(S - D) /[(S+D)I2]} x 100 ml) ml ml ml (acceptable range =:1: 10%) .:\\"5 1(.0 18.9 'Z.1/ I~) S I'Z.O ~ ,it t.' J D "lol." 110 ~.m t k So l Matrix sol e recoverv: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO,IL) (mg CaCO,lL1 (mil ml ml...,.....ml 'J:NSS 103'7 OIbO ~S '-If.l ~ 7."1 Ii) 10.~ 310

",5 Sample alkalinity (B)

Measured spike value (MY) % R=MV /SVx 100 (mg CaCO,/L) MV=A-B (acceptable range (mg CaCO,IL) "" 75 to 125%) lit> ~Cli:I IC6,OYo amme measuremen 'S: Sample volume Begin End Total Alkalinity Sample uumber SamnlelD . (mil ml ml ml Multinlier tm. CaCO,IL)

11. t,<:.. II'.0 1ioIc.. r()

Z dI'S -.:1." ~....l '2.'7 ('I) \\0*., 110 I 1. Cl~I1..,01. J., 3 J., 11.5 3'f."/ '2." I I'l.o I 'l.oc:. ", oS ~rcPl nt""'& I Z JIo/.1J '10.3 1:.'1 ISO) '3IDO \\1. UbIO.OS v I '2. "1o.:s Cle;. " c;, lZ.oo

  • ?Cl<"I'l.. 6S, l

3 0.0 ,.\\ '.\\ '3t.oC) I" O<'./ll:, 'Po ~cJo,billt. 1 '.1 10.1 '1.0 ~IClO I'J "(./h./l(,, z.. 16.1 1"1./ 't.O l:1~~ 1'7/l<' "

  • 0("

3 11.\\.1 I' q 3.'5 I' ~OOCl 126<;'Ol.0'-l AACQ.1w ( 50 17.'1' 1.1.S 3., ('1) >/I 7S I I Reviewed by: Date revIewed: IDS' 12.*11. SOP C6 - Exhibit C6.1. revision 06-0 I-II

Page 77 of 110 Titrant normalitv and multiplier determination: _.e!I of Titrant Normality Normality (N) ofH,S04 pH Factor or Multiplier DelOD check Begin End Total = (5 ml Na,CO, x 0.05)/E = (N x 50000)/100 ml sample water rei'ere>rr-standard ml ml ml -0.25/E - Nx500 4.5S.U. number nnm IE) (acceptable range 0.0180 - 0.0220) ~ OS*I'Z*I'Z Page 3'5 Page 11 of--,,~,--_ Time initiated 1-...............

==--=:,...---

Time completed ~ C\\lrI.4t. Titrate samples to pH =4.5 S.U. Alkalinity (SM 2320 B) Matrix: Water, RL = 1.0 mg CaCO,/L Analyst 1--".:s;...,;SS:::..---- Date analyzed ...;o",s,",.:.>.n~'I",t.,---__ ~ @>==-~=-::-:-=- o£nvlronmentalTestlng Solutions, Inc. I Laboratorv control standard: Reference standard True value Sample Alkalinity (MV) % RS-MV ITVx 100 number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range (mg CaCO,IL) (ml) ml ml ml = 90 to 110%)

rN$S 1037 100 100 Z,I.S 3\\.'l

't.? \\0,4 101 101.0% Duplicate samPle precISIon: Sample Alkalinity %RPD= Sample ID volume Begin End Total Mnltiplier (mg CaCO,IL) <<S - D) 1[(S+D)/21l x 100 number................ (mil ml ml ml ~ S Dnplicate (B) D Matrix spike recoverv: Reference standard Spike value Sample ..........~ Spike alkalinity (A) number (SV) volume Begin End Mnltiplier (mg CaCO,IL) 1m. CaCO,IL) (mil ml ml ~ Sample,dkalinity (B) Measnred spike valne (MV) ~XI00 (mg CaCO,IL) MV=A-B (ace ble range (m. CaCO,IL) =75to %) ~ It*R. Sample measurements: Sample volume Begin End Total Alkalinity Samnle number Sample ID - (mil ml ml ml Multinlier (mg CaCO,IL) I" n<:. I n.N Draro Ivr ~ 6D 31.t. 'J'l., Jot! '1.'1 10 If "71 I" l'I<:.".O" L 3 -\\; 3'1.' 3t.~ '1.' .L 75 n<'.I\\.\\'~ InHC" LllP ICO 38.2 '1'1. ~ '.0 ,,/ CZ ~ ,_ 0$.12.. 12. ReViewed by: Date reviewed: I OS

  • IZ *11..

SOP C6 - Exhibit C6.1. revision 06-01-11

Page 78 of 110 Analyst 1-...::sG:..=--- Date analyzed .S,O$* I'Z Total Hardness (8M 2340 C) RL = 1.0 mg CaCO,/L Page' --"'~'----_ Page of--'--__ Time initiatedI 01J~ Time completed __0;;..'1.;;5",',,- Titrant normalitv and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml =O.Z/E =(N x 50000)/50 ml sample number number (E) (acceptable range = 0.0180 - 0..0220) =Nx 1000 S\\ll "'ft ~NC'\\\\~ 0.0 lO.O '0.0 0.0,2000 ZO.O It d I Lb a oratory contro stan ar : Reference standard True value Sample Hardness (MV) %RS-MV/TVxIOO number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range (mg CaCOJIL) (mil ml ml ml = 90 to 110%) XN,ss \\033 40 50 \\0.0 IZ.O Z.O ~o.o 4(0 100.°% recision: Sample Hardness %RPD= Sample Sample ID volume Begin End Total Multiplier (mg CaCOJIL) {IS - D) 1[(S+D)/2]} x 100 number ml) ml ml ml 050 12-SSW SO It.o 1'1.0 a.o ~.() S t.IO ,I.. Duplicate (B) D 40 l'to IC.o ~o M.tix 'k S l ar SOl e recoverv: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (m. CaCOJlL1 (mil ml ml ml

II.ISS 1033

~O SO 1<./.0 '4.0 .... tl 2:0.0 'Sf> Sample hardness (B) Measured spike value (MV) % R-MV ISVx 100 (mg CaCOJIL) MV=A-B (acceptable range (mg CaCOJIL) = 75 to 125%) "to '{O 100'09'. amme measurements: Sampie volume Begin End Total Hardness Samole number Sample ID - (mil ml ml ml Multiplier (m. CaCOJIL) TV=ND Blank 50 0.0 WI) (should be - 0 m. CaC031L) O.Q ".0 'ZOo () O'\\S-DJ.12. l'h I5.IAJ 1$,0 n... 't.ll 1119' 0'0-o'6*,zJI 1.'2.1\\

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Page 79 of 110 %RPD= {(S - D) l[(S+D)l211 x 100 1'1 Spike hardness (A) (mg CaCO,IL) Page _-'---_,of & Time initiated If-.!o01aJ'c::li/l----'-~.I. Time completed __1-=!"'2"'3=- s D I'".12..0 ".1 'Z.o Page --,,7__ Total Hardness (8M 2340 C) RL ~ 1.0 mg CaCO,/L Begin End Total Multiplier ml ml ml 1'1.1 It.\\ 1.1.0 l'l.~ Hardness Begin End Total Multiplier (mg CaCO,IL) ml ml ml 50 Sample volume ml Sample volume (mil a d SamplelD recision: I Duplicate (B) Sample number iii ~ ~,=,'-"m-,m-,-,,,-"-,,"-,,-S-----'. Analyst 1_....:)$= Date analyzed _CS.:.=:...._I'3.=....;.I:..:'2.=-__ L h Reference standard Spike value numher (SV) (m. CaCOJIL) Matrix spike recovery: Titrant normali(y and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference .standard ml ml ml =O.2IE = (N x 50000)l50 ml sample number number iEl (acceptable range = 0.0180 - 0.0220) =Nx 1000

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Page 80 of 110 Titrant normality andmultiplier determination: ~_. Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference stanoalU ml ml = 0~2/E =(N x 50000)/ 50 ml sample number number ~ (acceptable range = 0.0180 - 0.0220) =Nx 1000 ~_'3*'t Analyst I_~~ Date analyzed oS*'3-t~ Total Hardness (8M 2340 C) RL = 1.0 mg CaC03/L Page _'=-_ Page ----""Z"'--_of Time initiatedE I Time completed ~~: oS'I'3'l't a d I Lb a oratory contro stan ar, : Reference sta~dard True value I Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Tot~1 Multiplier (mgCaCOJ!L) (acceptable range (mg CaCOJ/L) (mO ml ml ml = 90 to 110%)

S:~ 1033 40 50

".S. '-S.S ~.c> '1,t Coto 100.°" recision: Sample Hardness %RPD=.. Sample ID volume Begin End Total Multiplier (mg CaCOJ/L) {(S:. D) /[(S+D)/2]) x 100 ml ml ml ml 2.. &0 '1$.5 'lct.4 ....&1 s *87 D '97 l'.1 t't.3 ....44 Ml Ok atrIX SPI e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJIL) (mgCaCOJ!L) (ml) ml ml ml 'XNS.s.'0;,3 tto So '1..1 1.'.1 ' (..'t'. "." \\10 Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCOJIL) MV=A-B (acceptable range (m2CaCOJ!L) =75 to 125%) ,t{1 &(3 . 'o7.S~o t S l amp. e measuremen 'S: Sample volume Begin End Total. Hardness Samp*le number Sample ID (mi) ml ml ml Multiplier (mt! CaCOJIL) TV-?~ Rhmk ~OItI3""t (should be omil; CaC03/L) I.... f1 O~..tn.r, 'Z. A m"~lhJ l So 1.'.3 30..7. r.tt'" C? 6t;...(M-,'1- ~ 30.7 3S.t tof.S .tt t)S*b4\\"I1..A 4 15.l. 11.7.....5 I' os-n.. tLA 6 31.."7 "".1 *....5 SCI ~*U"'1.& ...t ~ e.te.t. 'Z ",.7 ...*.5 aCl' '~6__cn.. a' (IA ~~tJ'O\\ \\ 0.0 '1'.1 ct.\\

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Page 81 of 110 Time initiated I............. I Time completed ~ oS"J.ll eEl l!I @ ~_:o, ~ .~ Envlronment~rTestJng SolutIons, Inc. Analyst 1_-';:)1$=__---1 Date analyzed _~-=-.l",3'-'l:.."t.=--_--, Total Hardness (8M 2340 C) RL = 1.0 mg CaC031L Page Page 1

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Note: If >15ml oftitrant "used. sample must be dduted. ReVIewed by: I ~(<J;)Q Date reViewed I l)!>.I'~'l"Z_ SOP C7 - Exhibit C7.1. revision 06-01-11 d d I 'k L h Du Iicate sam Ie recision: Sample Hardness %RPD= Sample Sample m volume Begin End Total Multiplier (mg CaCO,IL) {(S* D) 1[(S+D)I2]J x tOO number A ml ml ml ml $\\J Iwr 1-1t.'J nit *u ~'*f s il 1"1.0S 0 ~ Duplicate (B) .y D 1$1 2~.q 't,.s 'tol 'V Titrant normalitv and multiolier determination: rmaIitv check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard m. m. m. 0.2/E (N x 50000)/50 ml sample number number IE) (acceptable ran -0.0220) Nx 1000 ~...CI$*I'*I'Z. S t a oratory contro stan ar : Reference standard True value Sample Hardness (MY) %RS=MY/TVxlOO number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range (mg CaCO,IL) Iml) ml ml ml = 90 to 110%)

rNSS tc>:J3 40 50

".3 It. '3 't.o lif** '10 '0(>.0% air/X SPI e recovery: Reference standard Spike value Sample Spike harduess (A) number (SV) volume Begin End Total Multiplier (mg CaCO,IL) 1m. CaCO,IL) Imll ml ml ml J:NSS 1633 '10 5"0 ~'Z.'t foe." 'Cl let. S l'ZoO Sample hardness (B) Measured spike value (MV) %R-MV/SVxlOO (mg CaCO,IL) MV=A*B (acceptable range 1m. CaCO,IL) ~75 to 125%) il 3" Q7.S% amPle measurements: Sample volume Begin End Total Hardness Samole number Samolem Imll ml ml ml Multiolier 1m. CaCO,ILl T Rlonl< ~OS'IHZ (should be. 0 m a, \\.,ne. I'. '2.\\ -r'A-SQ"':~ 3 50 ?8.~ 137.... '1.0 l't.t' ?r '7 ~,,"LII I ,vA $......0. Uli I 32.1{ 3',5 't,I fl 110'SO<;.1.~ I Z. 1,.5 LlO., 4.1 81 \\7.1Ic.. n.?f'I 3 ~O.' 1ol'4*.., ",.I III ,.,...e:-,..,.n ii/A ~tJlOlmu\\l1 4....7 'It.t If.I 81 I-z.OSO"'.'1.'- I 2. c..o ....0 10(.0 7" l'1.tI"'II.? I .l-3 . "1.0 ~;I 'I .7""'...... "'" "filA wB/\\) 10 I I ~.l It.S '1.1{ (/7 1'1.tl c;. 11'\\.1.1 J z. ~I, 1'Zo.S te. 'I lj.1.{ V ~? I I I I I I I I I I I I I I I

Page 82 of 110 Time initiated,............... I Time completed "S\\. O.$'."'.R. Total Hardness (8M 2340 C) RL ~ 1.0 mg CaCO,lL d d d ['r 1 Analyst1---'--:r0---- Date analyzed ---=c>::;S=.'..:c"=.'..,Ic::t.,--_ recision: Sample Hardness %RPD= Sample SampleID volume Begin End Total Multiplier (mg CaCOJiL) {IS - D) 1[(S+D)i2]} x 100 number ml) ml ml ml .1;1-"lilA weN 101 ~ So u.'1 '1..5 \\U s fer 11.'1 l-Duplicate (B) .L ~ 0 '2.1.'1 1.1.'l ll'l Page 10 'iil Page e.{: of, 'B L b t s [ Titrant normality an mu ttouer determination: .I:ttrant Normality check Begio End Total Normality (N) of EDTA pH Factor or Multiplier reference ml ml ml 0.2/E (N x 50000)1 50 ml sample number number (acceptable range 0.0180 -.0.0220) Nx 1000 ~ON1'1~ Note: If >15ml oftitron! is used, sample must be diluted., ReVIewed by: I__--j*~~--- Date reviewed I Oi.*I~. n_ SOP C7 - Exhibit C7.1. revision 06-01-11 a ora orv contro stan ar : Reference standard True value Sample Hardness (MV) % RS - MV lTV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOJiL) (acceptable range (mg CaCO,IL) (mil ml ml ml - 90 to 110%) .~s.s 1013 40 50 IC."I I$.'I 2.0 1"I. t. 40 100.0% Matrix soike recoverv: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin Eud Total Multiplier {mg CaCO,IL) (me CaCOJiLl (ml) ml ml ml ..t'~a.s \\03:3 4e:. I So 13.'1. Z'l*' '.I.f 1'I.f 130 Sample hardness (B) Measured spike value (MV) %R-MV/SVxIOO (mg CaCOJiL) MV=A-B (acceptable range (me CaCO,IL) = 75 to 125%) 8'1 '41 10'2:.5% Qmme measurements: Sample volume Begin End Total Hardness Sample number SampleID ' (mil ml ml ml Multiplier (m. CaCOJiLl Blank 5U!> OS 1~*I"C.. (should be 0 mg CaCOJiL) \\'1.()\\tn,o'i 'hi 1\\ WB.-) IUII\\lT", 50 'l.'I.8' 'S't.1 4.3 1.i' 35 ,.. ~"".1.2' 2. 3";/ .l8.s &1.'/ "\\7 \\ Z.OS\\\\. 'I.~

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Page 83 of 110 Titrant normalitv and multiplier determination: ...J:.i!rant Normality check Begin End Total Normality (N) ofEDTA pH Factor or Multiplier reference d ml ml ml 0..2/E (N. 50000)/50 ml sample number number lEI (acceptable range 0.0180 -.0.0220) NxlOOO ~()S*U*\\~ ~ET

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~ EnvlronmentaITestlngSo[utlons, Inc. Analyst 1-'-':l1"-'\\.B""----- Date analyzed ---=0",5_",-,1,-'.:..t.=.....__ Total Hardness (8M 2340 C) RL ~ 1.0 mg CaC031L Page --'.jll<-_ Page S of---"---_ Time initiatedI"==- I Time completed. ...........:!I'\\R qs. I"'£. Note: If >15ml oftttrant is used, ~ample must be diluted. RevIeWed by: I__~d-\\<;.I-,I--_- Date revIewed I 0 ~. \\.. ' \\"'L.. SOP C7 - Exhibit C7.1. revision 06-01*11 Laboratorv controlstandard: Reference standard True value Sample Hardness (MV) % RS - MV lTV. 100 number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range (mg CaCO,IL) (mn ml mI ml - 90 to 110%) %/olas 103'5 40 50 110.\\' ll.7 I.'I 14.9 31 'i$.0% 'k Sample Hardness %RPD= volume Begin End Total Mnltiplier (mg CaCO,IL) (S - D) 1[(S+D)121J x 100 mI ml ml ml n.* 0.1 S ".0 1 '1:1..1 '"'Z., D 'i.O n., 0.1 MatrIX sp, e recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Mnltiplier (mg CaCO,/L) (m. CaCO,IL) (mI) ml ml ml r~s 10'33 lib loS l.l..i' t'l.'I 1:.1 11>l'Mt '3 Sample hardness (B) Measured spike value (MV) % R=MV ISV. 100 (mg CaCO,IL) MV=A-B (acceptable range (mo CaCO,IL) = 75 to 125%) ~.O "7"1 '1'.~ Samole measurements: Sample volume Begin End Total Hardness Sample number Samnleill -(mn ml ml ml Multinlier 1m. CaCO,/L) ,~" -~~ Blank -....05*'3*'2, (should be 0 mo CaCO,IL) \\"1. O~ln. tn iOflUYlIi.llI ~ So t'l.'t ~S.1' _ 0.'1 I,.i' It \\ '2.flc;.,") *In .J, -3 l-$., U.'7* 11.'1 18 \\"1. Q$.OI. '0 ~t"\\CA-~ \\ "l.'-' ,"1.' .%.'1 57 \\1..lF-.II... 0'\\ I 2- ~1.' ')l.3

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Page 84 of 110 Page 1"1-of--,',,-_ Page Time initiated E:;;:: I Time completedc= ---:r~ 0$0 "*I~ Total Hardness (8M 2340 C) RL ~ 1.0 mg CaCO,1L recision: Sample Hardness °.loRPD = Samplem volume Begin End Total Multiplier (mg CaCO,IL) (IS

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. ml $'6 1.,\\ '.S 4.' s "II D "I 11.1 ~ QS*I3-rl. t I / It d l L b / s Titrant normalitv and multiplier determination: TUrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference s ml ml 0.2/E (N x 50000)/50 ml sample number number {" ccentable range 0.01S0.0.0220) Nx 1000 QS*I!*I"Z Note: If >lSml oflitrant is used. sample must be diluted. Reviewed by: I-----,J...C--- Date reviewed I 0$.\\"\\-\\1-SOP C7 - Exhibit C7.1. revision 06*0I-II a ora orv con ro s an ar : Reference standard True value Sample Hardness (MV) % RS - MV ITVx 100 number (TV) volume Begin End Total Mnltiplier (mg CaCO,IL) (acceptable range (mg CaCO,IL) (mil ml ml ml = 90 to 110%). "%NSS 101";$ 40 50 6.0 1.'1 1*1 \\'1.'6 lS 'is.O% Matrix spike recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Mnltiplier (mg CaCO,/L) (mo CaCO,/L) (mil ml ml ml ":l:'NoSS 1033 ,",0 Sc> '.5 13.1,., l'l.t I~O Sample hardness (B) Measured spike value (MV) % R=MV ISV x 100 (mg CaCO,IL) MV=A*B (acceptable range (mo CaCO,JL) =75 to 125%) 'II 3' "17.5% amme measuremen 'S: Sample volume Begin End Total Hardness Sample number Sample ID -(mil ml ml ml Multiplier 1m. CaCO,IL\\ T" .,~ Blank at8 OS*'3*1"t. (should be omg CaCO,IL) 11.0C,\\'2,. n \\ FiN.uwd -;J So '"3.1 \\1.3 &.l.z. ,,,.~ f3 ':.J 'OO,,' ".o~ fhlrllY1 I 1"7.3 '8'.'1 1.1 Z~ \\ L 0(, Itl * /t1. I l-lSi." ~I.s 3.\\ C/ "_l'ftl1..O'L J, 3

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Sequoyah Nuclear Plant Biomonitoring May 08 - 15, 2012 Appendix D Reference Toxicant Test and Control Chart Page 85 of 110

Page 86 of 110 tiPo ~ Pimephales promelas Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc. Environmental Testing Solutions, Inc. USEPA Control Limits (+/- 2 Standard Deviations) USEPA Warning and Control Limits (75th and 90th Percentile CVs) Laboratory Warning and Control Limits (10th and 25th Percentile CVs) 0.9 0.8 0.7 0.6 0.5 1.4 1.2 U ~ 1.0 'Do-0.8 Ul N U 0.6 III "'C I 0.4 1.4 1.2 1.0 0.8 0.6 0.4 Test date Graphs senerated from associated excel spreadsheet. Excel spreadsheet ent ad by; J. Sumner Reviewed by; 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC2s) Warning Limits (mean IC25 +/- SA.10 or SA.7S) Control Limits (mean IC25 +/- SA.25' SA.90- or 2 Standard Deviations)

Page 87 of 110 ~E* T* .. S. ~ "."..;'"! .~. ITesting Solutions, 'no. \\SJ Envlronmenta Pimephales promelas Chronic Reference Toxicant Control Chart State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-dayl~ CT S Control LImits S.. Wilming Umlts SA.2S Control Umlts SA.1S Warning Limits s.~ Control Umits CV (giL KCI) (gIL KClj CT-2S 0+25 CT-SA.lD cr +54.10 CT -S,Q,.2S CT+SA.Z5 CT - SA.75 CT +SA.75 cr -SA-90 CT+SA.9D 1 03-29-11 0.75 0.72 0.04 0.64 0.80 0.09 0.63 0.81 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.05 2 03-31-11 0.75 0.72 0.04 0.64 0.80 0.09 0.63 O.SO 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.05 3 05-03-11 0.78 0.72 0.04 0.64 0.80 0.09 0.63 O.SO 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.05 06-07-11 0.76 0.72 0.04 0.64 0.80 0.09 0.63 0.81 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.05 5 06-14-11 0.63 0.71 0.04 0.63 0.80 0.09 0.63 O.SO 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.04 0.06 6 07-12-11 0.67 0.71 0.04 0.63 0.80 0.09 0.63 O.SO 0.15 0.56 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.06 7 07-19-11 0.63 0.71 0.05 0.62 O.SO 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.06 8 08-09-11 0.67 0.71 0.05 0.62 O.SO 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 9 09-13-11 0.66 0.71 0.05 0.61 O.SO 0.08 0.62 0.79 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 10 10-04-11 0.73 0.71 0.05 0.61 0.81 0.09 0.62 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 11 10-11-11 0.76 0.71 0.05 0.62 0.81 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.03 0.07 12 10-18-11 0.67 0.71 0.05 0.61 0.81 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 13 11-08-11 0.78 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 1. 12-06-11 0.77 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 15 02-07-12 0.77 0.71 0.05 0.61 0.81 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 16 02-14-12 0.74 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.04 0.07 17 03-06-12 0.64 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 18 03-06-12 0.68 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 19 04-12-12 0.71 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 20 05-08-12 0.77 0.72 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.57 0.137 0.27 0.44 0.99 0.32 0.39 1.04 0.08 Nate: 7-d ICzs = 7-day 25% inh'lbition concentraflon. An esflmation of the concentration of potassium chloride that would cause a 25% reducflon'ln Pimephales growth forthe test population. CT = central tendency (mean IC2s). S = Standard deviation of the lG.ls values. laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile OJs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. SA.1O = Standard deviation corresponding to the 1f!' percentile CV. (SA.lO = 0.12) Sus = Standard deviation corresponding to the 2.'1' percentile OJ. (SA.25 = 0.21) USEPA Control and Warning Urnlu SA.1S = Standard deviation corresponding to the 75 th percentile OJ. (SA.1S = 0.38) SA.90= Standard deviation corresponding to the 9dh percentile OJ. (SA.90 = 0.45) CV = Coefficient of variation of the lG:s values. USEPA. 2000. Undemanding and Accauntingfor Method Variability In Whole EffluentToxicity Applications Under the National Pollutant DIscharge Elimination Program. EPA-833-R-Q0-Q03. US Environmental Protection Agency, Cincinnati, OH. File: ppkclcr_050812.xlsx Entered by: J. Sumner Reviewed by:--df-

Page 88 of 110 ~o ~ Environmental Testing Solutions, Inc. Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source: Aquatox, Inc.. ~---:: -- USEPA Acceptance Criteria (> 0.25 mg per surviving larvae) 20 30-------------------------- Kentucky Acceptance Limit << 30.0%) o 10 ~- l:.c 0... lQ 0 ~ Cl- "6 0..... l: l: 0 GI U

  • u..

e.e GI0U 30 USEPA Upper PMSO Bound (90'h percentile < 30.0%) 20 10 Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) Graphs senerated from associated excel spreadsheet. EKcel spreadsheet ellte~ J. Sumner Reviewed by: -----w~~

Page 89 of 110 ~ETS j EnvlronmentalTestlng Solutlons,lnc. Precision of Endpoint Measurements Pimephales promelas Chronic Reference Toxicant Data Test number Test date Control Control Mean CT CV CT MSD PMSD CT Survival Growth 1%) (mg/larvae) for Control Growth (%) for Control (%) for PMSD (%) (mgJlarvae) Growth CV (%) 1 03-29-11 100 0.829 6.3 0.09 10.3 2 03-31-11 100 0.675 0.752 3.2 4.8 0.09 12.8 11.5 3 05-03-11 100 0.867 0.790 6.3 5.3 0.08 8.8 10.6 4 06-07-11 97.5 0.839 0.802 5.8 5.4 0.11 12.7 11.1 5 06-14-11 100 0.758 0.793 14.5 7.2 0.13 16.8 12.3 6 07-12-11 97.5 0.872 0.806 6.0 7.0 0.09 9.8 11.9 7 07-19-11 100 0.839 0.811 5.4 6.8 0.06 7.2 11.2 8 08-09-11 100 0.662 0.792 6.6 6.7 0.07 10.6 11.1 9 09-13-11 100 0.848 0.799 6.7 6.7 0.12 13.6 11.4 10 10-04-11 100 0.768 0.796 16.8 7.7 0.13 16.9 11.9 11 10-11-11 100 0.681 0.785 4.3 7.4 0.07 9.9 11.8 12 10-18-11 100 0.693 0.777 4.8 7.2 0.07 10.0 11.6 13 11-08-11 100 0.792 0.779 10.3 7.5 0.12 15.6 11.9 14 12-06-11 100 0.738 0.776 15.7 8.0 0.12 15.9 12.2 15 02-07-12 100 0.662 0.768 10.5 8.2 0.09 13.4 12.3 16 02-14-12 97.5 0.658 0.761 5.2 8.0 0.07 10.1 12.1 17 03-06-12 100 0.854 0.767 3.5 7.8 0.09 10.8 12.1 18 03-06-12 100 0.873 0.773 5.1 7.6 0.10 11.4 12.0 19 04-12-12 97.5 0.706 0.769 7.0 7.6 0.06 8.7 11.8 20 05-08-12 100 0.613 0.761 8.1 7.6 0.08 13.1 11.9 Note: CV = Coefficient of variation for control growth. Lower CV bound determined by USEPA (10th percentile) '" 3.5%. UpperCV bound determined by USEPA (90th percentile) =20% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure attest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. lower PMSD bound determined by USEPA (10th percentile) '" 12%. Upper PMSD bound determined by USEPA (90th percentile) '" 30%. cr '" Central Tendancy (mean Control Growth, CV, or PMSDj USEPA. 2000. Understanding and Accounting for Method Variability In Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA*833-R-OO-o03. US Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA*821*B*01*004 and EPA-821-B-Ol-00S. US Environmental Protection Agency, Cincinnati, OH. I.d'pend.", Ral'fowbV Kell'v'*Koen," \\~I~ File: ppkclcr_050812.xlsx Entered by: 1. Su~r Reviewed by:---------19'

Page 90 of 110 "ET ell w* I §),$:'1~r:;':¢ if;;; ~: ~ Envlranmental Te$ting Soilltions, Inc. Page I of5 PpKCICR Test Number: 1.-1~ Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas SOP AT21 - Exhibit AT21.1, revision 06-0I-II MHSW batch used Anal st Time Test initiation, renewal, or termination Anal st Acceptance criteria Time Anal st Time Morning feeding Afternoon feeding Dailyfeeding and renewal information: Avera e wei t er initiallarvae: Average weight per surviving larvae: % Mortali Control in ormation: Day Date 0 OS-O~' !"t. ~.

  • ,"1-2 0$-/0'"

3 OS*I\\*I'Z.. OS*I"l..*I'l.. 5 O~'I 6 OS*, -I'L. 7 OS'l -11. Dilution oreoaration information: Comments: KCl Stock INSS number: IIIlS<' 10'l1c. Stock preparation: 50 g KCIIL: Dissolve 50 g KC! in l-L Milli*Q water. Dilution prep (mgIL) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 .982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test oreanism information: Test information: Organism age: 7n.l. L\\A.wtC. t" b Randomizing template: b~tJ Date and times organisms ~.111' \\"'L '\\coCO Incubator number and ~'t> were born between: shelf location: Organism source: AThlC. 6A1\\.~\\ Pf O...*(T\\*'"L Artemia CHM number: (MM IoS'1-Drying informationfor weight determination: Transfer vessel pH-1."' S.u. Date / Time in oven: 0" 'S'll 1Il/0 information: Temperature ~ 1.'1.& 'c Initial oven temperature: tao*t Average transfer volume: O.\\"to'\\.....1l Date / Time out of oven: .,;..,..., l.. 11'10 Final oven temperature: Ian' ( Total drying time: "lJ.!.l1n.IoI( Ind,p..,don, n....rewbv J(oll.ve.~ **n.n v:- I I I I I I I I I I ~ I I

Page 91 of 110 Day Control 450 m. KCIIL 600 mt KCIIL A a C D E F G H I J K L 0 10 1O 10 It) 10 10 10 10 If) 10 10 10 1 10 10 10 10 10 fa 10 10 /0 10 10 fO 2 It) '0 10 1O 10 '0 10 to /0 10 10 /0 3 10 If) 10 10 10 10 fO 10 /0 10 10 If:> 4 IC) to Itl It) 10 10 10 10 10 10 10 10 5 Ie> If) II) /0 10 '0 I() It) 10 10 '0 If:> 6 /D If) I() 10 10 JO 10 10 ID fO to 10 7 It> /0 10 10 If) It) ID Ie '0 10 16 10 A Pan weight (mg) (I . Tray color code:: I)....... q}"y,\\,.,,!un\\ Analyst: Mitt 14.1,8 1'5.Se. I~. \\'5 13.1t 1'!>.'l4 It.'1s 13.'51 14.4-1 1!>."I'Z. IS.I1-I!>. 1.1.- 13.'S1 J)ate: 0'5.04.12 B - Pan + Larvae weight (mg) Aoalyst: fl1):r ~.36 11,01- /1.31 IQ.1f 20,10 2D./Q l.~f, ZD:SJ ~O.lfe l.I.sc, let.'¥f Z/).'v'z. Date: 06:i'tj1 C - Larvae weight (mg) - B - A Hand calculated.4 ...10 'S...., r.:ZI.. lo.n. (,.~, '.a'l S."I1 S.f'I r..l>Io 10.1'\\ 1...11 7./1 Analyst:

  • e Weight per initial number oflarvae (mg)

= C I Initial number of larvae \\D~ ~ ~ 4-~ ~\\D ~ JIo,<:l ~ ,if "to \\.a.- t-Hand.calculated.OJ \\II &ii 'Ii C; \\II \\0 1'\\ Analyst: ~ C' 0 b' 0' 0' ~. ~. I)' 0' O' O' ()' Average weight per* initial Percent number of larvae (mg) reduction O.I.I~ O.lo~~ -~.~7, o.",~1 -10,'2.7, from control (%1 Comments: PpKCICR Test Number: ~ Survival and Growth Data SOP AT21 - Exhibit AT21.1, revision 06-01-11 Species: Pimephales promelas Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk ~ sick, sm =unusually small, Ig ~ unusually large, d&r = decanted and returned, w ~ wounded. I"d.pond'.' ROVIewby ~.II*VE.~ **n.n b I I I I I I I I I I I I I I I I I" I ~ Ii

Page 92 of 110 Comment codes: c =clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, 19 = unusually large, d&r = decanted and returned, w =wounded. Day 750m KCVL 900m KCVL 1050 m KCVL M N 0 p Q R, S T U V W X 0 It> 10 Ie 10 10 10 10 10 10 10 10 10 1 8~ ,J. .(, 'l~ .'\\ct '/$"14,., ?:' -S""

..$01,. '"'" ~

'\\ I:l '\\ to (., 5 SO'"~ 2 g q q '\\ 10 t., g ~'o\\ ?:JIIJ... ?:J1.G\\. ~'1~ ~t<\\ 3 &'A 1\\' q sill. S'<t,'.{ 5 '2'0\\ ' "I- -?> ~ "2-4 g ~ '\\ t:J... 5 I,{ '\\ 'S S 1.. .3 -3 5 ~ 'I '\\ L\\ U(. ~ ~ .3 \\'loi g fc:, \\ 1-6 g'" v\\ -z... '~ 8 '\\ "\\ ~ S \\ \\ 7 ~Io\\ 1'" 4 Uo s \\.", \\ I~ \\\\,,&0 S '5 \\ A - Pan weight (mg) ( Tray color code:: OAtIL!:J,,,,, bm4r0 Analyst: 1\\1"* IS.lI'! 1'3.~O 1'3.14-1t..:}O 14.'-Z 13:5z 14.0'; 14.0$ 13."l1 13.44-14.B'I II}.t.'l Date: QS*p4.."z. B - Pan + Larvae weight (mg) Analyst: ~~ ZM/z. 18.'111 '6."Il rM.El '8.01{ IlA'02-11-:03 11-.~\\Q I~.\\f"l /'I.tel 1'5.1QO 15.1J, Date: ~l2L C - Larvae weight (mg) - B - A Hand calculated. <9}. s.~ If.'11. 'l.'~ ".5& !."l'2. 3.S0 3.1~ ~..'11 0.1& O.t"4 0.1\\ ~.11 Analyst: Weight per initial number oflarvae (mg) = C I Initial number oflarvae ~ ',,~.~ ~ ~~ ~ t ",l!1 ~ ~ ~~ ~\\) Hand calculated. ..\\"1.-- '!J 0'[:1 ~ ,)'; Ilj ()' Analyst: 0' ~. O' O' O* ~. t>' 1;)' C' C' Average weight per'"initial Percent number of larvae (mg) reduction 0.,\\,\\-5 It\\. '11. 0.1105 &.Io.'H 0, o~1 8r..11. from control (%) rI k' Wi _1 l' I I I I I I I I I II I 'I, t ",,\\ ~,. °ETS °" I II ' IA'i;~,?! i~ '~'f:::",{('f;' ~ Enviranm~nlaITe5t1ng Sallltians, rn~. Species: Pimephales promelas Comments: In~.p..,d"'l RovIowbV K.IIO'f~,K**n.n ~ Page 3 of5 PpKCICR Test Number: ~ Survival and Growth Data SOP ATIl - Exhibit ATILl, revision 06-01-11

Page 93 of 110 @jD ~~f ti,,~ a ~I~~ Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 248 May 08-15, 2012 Test number:------ Test dates:---------=-----"-- Quality Control Verification of Data Entry, Calculations, and Statistical Analyses ConCllntl'lltlon (mc/L KCI RepllCllte Inltlel number of Rnel number of A =Pen weflht (mil B=Pen+"1'VIItI "Mle weflht (mil = Wellht / Survlvlnl Meen wellht/SUrvlvlnl Coelllcfent of VIlrfetlon Weflht / fnltlel number of Meen survlVllI ("I Mun wellhtf Inltlel Coefficient of VIlrfetlon PerCllnt reduction from IIIM1e lerwe weflht(mll A-B number of lerVll8 (mil number of leMle (mil IMellft.,..htper.urAvInI 'e_e(m,l number of larYlle ("I control ("I .umbo. of 10...1("I (mil A 10 10 14.68 21.38 6.70 0.670 0.670 Control B 10 10 13.56 19.07 5.51 0.551 0.613 8.1 0.551 100.0 0.613 8.1 Not applicable C 10 10 13.13 19.39 6.26 0.626 0.626 D 10 10 13.72 19.75 6.03 0.603 0.603 E 10 10 13.79 20.10 6.31 0.631 0.631 450 F 10 10 12.95 20.14 7.19 0.719 0.633 9.6 0.719 100.0 0.633 9.6 -3.3 G 10 10 13.39 19.36 5.97 0.597 0.597 H 10 10 14.47 20.31 5.84 0.584 0.584 I 10 10 13.92 20.48 6.56 0.656 0.656 600 J 10 10 15.17 21.36 6.19 0.619 0.651 6.8 0.619 100.0 0.651 6.8 -6.2 K 10 10 13.62 19.79 6.17 0.617 0.617 L 10 10 13.51 20.62 7.11 0.711 0.711 M 10 8 15.29 20.82 5.53 0.691 0.553 750 N 10 8 13.50 18.46 4.96 0.620 0.639 6.8 0.496 77.5 0.495 8.4 19.2 0 10 8 13.74 18.47 4.73 0.591 0.473 P 10 7 12.70 17.28 4.58 0.654 0.458 Q 10 4 14.62 18.04 3.42 0.855 0.342 900 R 10 5 13.32 16.82 3.50 0.700 0.773 8.3 0.350 47.5 0.365 6.3 40.4 5 10 5 14.05 17.83 3.78 0.756 0.378 T 10 5 14.05 17.96 3.91 0.782 0.391 U 10 1 13.91 14.64 0.73 0.730 0.073 1050 V 10 1 13.44 14.28 0.84 0.840 0.813 14.7 0.084-10.0 0.081 14.7 86.7 W 10 1 14.89 15.60 0.71 0.710 0.071 X 10 1 14.29 15.26 0.97 0.970 0.097 Dunnett's MSD value: PMSD: O.OBOO 13.1 MSD= PMSD= Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatmentthat can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10th percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile) = 30%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent TOXicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-Ql-004 and EPA-821-B-Ql-Q05. US Environmental Protection Agency, Cincinnati, OH. File: ppkclcr_050812.x1sx Entered by: J. S'f)fer Reviewed by:-et-

Page 94 of 110 1 2 3 4 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 0.8000 0.8000 0.8000 0.7000 0.4000 0.5000 0.5000 0.5000 0.1000 0.1000 0.1000 0.1000 ~~TS ~ Environmental Testing Solution" Inc. Start Date: 5/812012 End Date: 5/15/2012 Sample Date: Comments: Cone-mall D-Control 450 600 750 900 1050 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Test ID: PpKCICR Sample 10: REF-Ref Toxicant Lab 10: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Transform: Arcsin Square Root Rank 1-Tailed Cone-mg/l Mean N-Mean Mean Min Max CV% N Sum Critical D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 600 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 '750 0.7750 0.7750 1.0782 0.9912 1.1071 5.379 4 10.00 10.00

  • 900 0.4750 0.4750 0.7602 0.6847 0.7854 6.622 4

10.00 10.00

  • 1050 0.1000 0.1000 0.3218 0.3218 0.3218 0.000 4

10.00 10.00 Auxiliary Tests Shapiro-Wilk's Test indicates non-normal distribution (p <: 0.01) Equality of variance cannot be confirmed Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 600 750 670.82 Treatments vs D-Control Statistic 0.63088 Critical 0.884 Number Total Reap Number 0 40 0 40 0 40 9 40 21 40 36 40 Skew Kurt -2.1517 5.4494 Ind.p..d,ot RO'I1..... tIy t<elleye.Koona" ~.~: Maximum Ukellhood-Problt Parameter Value SE 95% Fiducial Limits Control Chi...sg Critical P-value Mu Sigma Iter Slope 14.4814 1.97334 10.6137 18.3492 0 1.78643 7.81472 0.61789 2.93943 0.06905 3 Intercept -37.567 5.80045 -48.936 -26.198 TSCR 1.0 Point Problts mg/l 95% Fiducial limits 0.9 EC01 2.674 600.876 521.365 654.429 EC05 3.355 669.645 602.697 714.916 0.* EC10 3.718 709.467 650.476 750.155 0.7 EC15 3.964 737.664 684.352 775.454 EC20 4.158 760.871 712.091 796.659 BI 0.6 0 EC25 4.326 781.362 736.336 815.805

8. 0.5 EC40 4.747 835.476 798.142 869.388

~ 0.4 EC50 5.000 869.818 834.745 906.603 EC60 5.253 905.573 870.247 948.43 0.3 EC75 5.674 968.289 927.107 1028.37 0.2 EC80 5.842 994.365 949.312 1063.47 EC85 6.036 1025.65 975.202 1106.65 0.1 EC90 6.282 1068.41 1008.03 1164.36 0.0 EC95 6.645 1129.83 1057.65 1256.74 1 10 100 1000 10000 EC99 7.326 1259.13 1155.35 1452.86 Dosemg/l Dose-Response Plot 0.9 0.* ~ 0.7 'E 0.6 tn 0.5 B0' ... 0.3 0.2 0.1 0 :g 0 0 0 0 0 ~ 0 ~ 0 ~ 0 w ~ ~ 0 8 r II File: ppkclcr_050812.x1sx Entered by: 1. Srr Reviewed by: ~

Page 95 of 110 ~~TS ,.) Environmental TOlSII"IISolldlon"lnc. Statistical Analyses 1 2 3 4 0.6700 0.5510 0.6260 0.6030 0.6310 0.7190 0.5970 0.5840 0.6560 0.6190 0.6170 0.7110 0.5530 0.4960 0.4730 0.4580 0.3420 0.3500 0.3780 0.3910 0.0730 0.0840 0.0710 0.0970 Start Date: End Date: Sample Date: Comments: Conc-mg/l D-Control 450 500 750 900 1050 5/8/2012 5/15/2012 larval Fish Growth and Survival Test-7 Day Growth Test 10: PpKCICR Sample 10: REF-Ref Toxicant Lab 10: ETS-Envlr. Testing Sol. Sample Type: KCL-Potassium chloride Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Transform:- Untransformed i-Tailed Isotonic Conc.mg/l Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6125 1.0000 0.6125 0.5510 0.6700 8.087 4 0.6320 1.0000 450 0.6328 1.0331 0.6328 0.5840 0.7190 9.612 4 -0.552 2.180 0.0800 0.6320 1.0000 600 0.6508 1.0624 0.6508 0.6170 0.7110 6.760 4 -1.042 2.180 0.0800 0.6320 1.0000 750 0.4950 0.8082 0.4950 0.4580 0.5530 8.425 4 0.4950 0.7832 900 0.3653 0.5963 0.3653 0.3420 0.3910 6.320 4 0.3653 0.5779 1050 0.0813. 0.1327 0.0813 0.0710 0.0970 14.714 4 0.0813 0.1286 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wllk's Test indicates normal distribution (p > 0.01) 0.92935 0.805 0.61878 -0.6846 Bartlett's Test indicates equal variances (p = 0.87) 0.28594 9.21035 Hypothesis Test (i*tail. 0.05) NOEC LOEC ChV TU MSOu MSOp Msa MS. F-Prob df Dunnett's Test 600 >600 0.08004 0.13067 0.00146 0.0027 0.59872 2, 9 Treatments vs D-Control linear Interpolation (200 Resamples) 1500 500 1000 Dose mg/l 1.0,-----------, 0.* 0.8 0.7 0.* al 0.5! 0.4 ~ 0.3 0.2 0.1 0.0 ~-."~""'""~_.-. -0.1 -0.2 +-._~~__~~_-.-I o Point mg/l SD 95% CL(Exp) Skew IC05 634.60 28.01 593.31 661.72 -7.8928 IC10 669.20 14.86 633.19 723.43 0.9209 ICi5 703.80 19.42 666.12 781.73 0.9450 IC20 738.39 20.46 694.18 806.57 0.4813 IC25 774.28 20.26 718.07 838.43 0.1632 IC40 883.87 12.92 841.40 918.61 -0.1823 IC50 926.01 5.72 908.42 943.16 -0.0795 Dose-Response Plot 0.8,-------~----_-------_, 0.7 0.8 l05 o .., 0.4 iI' ~ 0.3 0.2 1-tall,0.05Ievel of significance 0.1 0g 0 0 0 0 0 ~ 0 ~ 0 ~ ~ ~ ~ Q ~ 0"c Ind,p.ndent R..I b~ KelloyE.X n '"tt-File: ppkclcf_050812.x1sK Entered by: J. SlIJIUIer Reviewed by:~

Page 96 of 110 i I I I I I I I I I I i I I i I IIi I~ ~, tnd0f>l'odo" ROIllowbV KeliovE.Ke<n'n E Species: Pimephales promelas Concentration CONTROL ,,~. 450 mgKCllL 600 mg KCVL IMO 1.0.1.'" 750 mg KCIIL 1(,30 1.1.(."I 900 mg KCIIL 1050 mg KCIIL STOCK Initial Page 4 of5 PpKCICR Test Number: t"'& SOP AT2 I - Exhibit AT2 I. I. revision 06-0I-II

Page 97 of 110 Final Initial Final PpKCICR Test Number: ~ Page 5 of5 Initial SOP AT21 - Exhibit AT2I.l, revision 06-01-11 Final 81 eQI \\050 '1.'-\\*1 ~,,(li 1." nlo '1'-\\.S: 8.00 V> I':)\\.iIO 1.....& i~H)O 1.4 l'tl'60 l.LI.( 800 1.'-1 ?OSO '1.... JI 1.~.,\\ Initial Final Initial Anal st CONTROL "'~ Species: Pimephales promelas "ETS ,~ i:...~, ';~ 0.*'~....~... i1....*,'.' ~~.*..c"': )

~;!.
,":

~r: ~iJ,~ ~ EnvironmenialTI!5ting Solutions, Inc. Concentration Parameter 600 mg KCl/L 750 mg KCI/L 900 mg KCI/L 450 mg KCI/L 1050 mg KCI/L l,depOJ1dool ROIII.....bV KelloyE.K..n.n "P=- I I I I I I I II i I ~ I I I I I I

Page 98 of 110 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc. USEPA Control Limits (+/- 2 Standard Deviations) ~--

~t---

Laboratory Warning and Control Limits (10th and 25th Percentile CVs) USEPA Warning and Control Limits (75th and 90th Percentile CVs) 1.14 1.12 1.10 1.08 1.06 1.04 1.02 2.5 Q U 2.0 ~Z ~ 1.5 ~ 1.0 U.... ~ ~ 0.5 "0 It-- 1.4 1.3 1.2 1.1 1.0 0.9 Test date 7-day IC25 = 25% inhibition concentration. An estimation ofthe conceptration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. - -. Central Tendency (mean IC2s) _._.- Warning Limits (mean IC2s +/- SA.1O or SA.7S)

      • ..**.* Control Limits (mean IC2s +/- SA2S' SA.90' or 2 Standard Deviations)

GraphSllenerated from associated excel spreadsheet. Excel spreadsheet entered by: J. Sumner Reviewed by: ~

Page 99 of 110 rrt if ~t ili!l o '~~TS Environmental Testing Solutions, Inc. Ceriodaphnia dubia Chronic Reference Toxicant Control Chart State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day IC25 CT S Control Limits S'M Warning Limits S.~ Control Limits S'H Warning Limits SA.')O Control Limits CV (giL NaCI) (giL NaCl) CT-2S CT+2S cr -SA,lD cr + SA.to CT-SA.2S CT+ SA.25 CT - SA.75 CT+ SA.75 cr - SA.90 CT+SA.9o 1 01-18-11 1.06 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.56 0.66 0.41 1.74 0.01 2 02-08-11 lD' lD1 0.01 LOS 1.09 0.09 0" 1.16 0.18 0.89 125 OAS 0.59 155 0.66 0.41 1.74 ODl 3 03-08-11 LOS 1.07 am 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 4 ~5-11 1.06 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.Q1 5 04-05-11 1.07 1.07 0.01 LOS 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 05-03-11 1.07 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 7 06-07-11 1.05 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 8 07-12-11 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 08-09-11 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 p.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 10 09-13-11 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 11 10-04-11 1.07 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 12 10-11-11 1.06 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 13 11-08-11 1.05 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.01 14 12-06-11 1.06 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 15 01-10-12 lDB 1.07 0.01 1M 1.09 0.09 0.98 1.15 0.18 0118 1.25 OA8 OS. 154 0.66 DAD 1.73 DDt 16 02-07-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 17 03-13-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 18 04-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 04-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01 20 05-mH2 1.06 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01 Note: 7-d IC2S '" 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT '" Central tendency (mean IC2s). S'" Standard deviation ofthe I~ values. Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC2S values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by U5EPA for the test method and endpoint. Suo'" Standard deviation corresponding to the 10th percentile CV. (5A.1O '" 0.08) SA.2S '" Standard deviation corresponding to the 25th percentile CV. (SA,25 '" 0.17) USEPA Control and Warning Limits SA.7S'" Standard deviation corresponding to the 75t~ percentile CV. (SA.7S'" OAs) SA.9II'" Standard deviation corresponding to the 90th percentile CV. (SA.9Q '" 0.62) CV '" Coefficient of variation of the lC:!s values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-OO-003. US Environmental Protection Agency, Oncinnati, OH. File: CdNaCICR_050812.xlsx Entered by: JJlner Reviewed by:

Page 100 of 110 ~ETS j Environmental Testing Solutions. Inc. Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision ofEndpoint Measurements 40 Minimum Acceptance Criteria (> 15.0 offspring per snrviving female) = 35 0.. 'Q;' Col - = ~ 30 "Cl a o~ Q.i)J) 25 .. = ~....- Q. 0 20 .. = ....= 0 0 U 15 Kentncky Acceptance Limit << 30.0%) North Carolina Acceptance Limit << 40.0%) 30 10 20 40 40 USEPA Upper PMSD Bonnd (90th percentile < 47.0%) ~f 20 10 Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD) Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations) Graphs generated from associated eKcel spreadsheet. EKcel spreadsheet e:.~ed by: J. Sumner Reviewed by: ~

Page 101 of 110

  1. I'.'.'.

() j Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Ceriodaphnia dubia Chronic Reference Toxicant Data Test Test date Control Control Mean number SUNival Reproduction cr CV cr MSD PMSD cr for Control Mean for Control (%) (offspring/female) Reproduction (%) Reproduction (%) for PMSD (%) (offspring/female) CV(%) 1 01-18-11 100 31.9 4,8 2,0 6.3 2 02-08-11 100 30.7 31.3 6.3 5.6 2.3 7.4 6.9 3 03-08-11 100 32.6 31.7 6.9 6.0 1.8 5.4 6.4 4 04-05-11 100 32.8 32.0 4.8 5.7 2.0 6.0 6.3 5 04-05-11 100 33.0 32.2 5.9 5.8 2.2 6.7 6.4 6 05-03-11 100 30.9 32.0 4.5 5.5 1.9 6.1 6.3 7 06-07-11 100 28.9 31.5 6.7 5.7 2.5 8.7 6.6 8 07-12-11 100 32.2 31.6 6.0 5.7 2.0 6.2 6.6 9 08-09-11 100 28.3 31.3 5.0 5.7 1.9 6.8 6.6 10 09-13-11 100 32.1 31.3 8.2 5.9 1.9 5.8 6.5 11 10-04-11 100 32.8 31.5 6.1 5.9 2.1 6.5 6.5 12 10-11-11 100 31.3 31.5 3.0 5.7 2.1 6.6 6.5 13 11-08-11 100 29.0 31.3 7.6 5.8 1.8 6.1 6.5 14 12-06-11 100 29.3 31.1 5.8 5.8 1.9 6.5 6.5 15 01-10-12 100 32.4 31.2 5.1 5.8 2.4 7.4 6.6 16 02-07-12 100 31.7 31.2 4.2 5.7 2.1 6.5 6.6 17 03-13-12 100 31.1 31.2 7.2 5.8 1.9 6.1 6.5 18 04-10-12 100 29.4 31.1 6.8 5.8 2.4 8.0 6.6 19 04-10-12 100 30.3 31.1 7.1 5.9 2.7 8.8 6.7 20 05-08-12 100 31.7 31.1 6.1 5.9 1.9 6.1 6.7 Note: CV =Coefficient of variation for control reproduction. lower CV bound determined by U5EPA (10th percentile) =8.9%. Upper CV bound determined by USEPA (90th percentile) = 42% MSD =Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statisticallv significant in a whole effluent toxicity test. , lower PMSD bound determined by USEPA (loth percentile) =13%'. Upper PMSD bound determined by USEPA (90th percentile) =47%. CT =Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200la, 200lb. Final Report: Interlaboratory Variability Study of EPA ShorHerm Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-Ol~004 and EPA-821-B-Ol-00S. US Environmental Protection Agency, Cincinnati, OH. led.p,nd,n' RovlowOy ~.rlO'fE,Ke.nan ~ File: CdNaCICR_050812.xlsx Entered by: J. Sumner Reviewed by:+

Page 102 of 110 Page lof6 '~l j ~J J Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia -1 . J CdNaCICR #: \\0'"\\ Dilution nrenaration information: Comments: NaCI Stock INSS number: ~I Stock preparation: 100 g NaCIIL; Dissolve 50 g: NaCl in 500 roL Milli-O water. Dilution;;;enCn;<;m 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volumermL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 J J Test orllanism source information: Test information: O~anism age: < 24-hours old Randomizing temnlate color: Date and times organisms were born O"'~'I"\\- 0l0/oi0 "I1:l 01llO Incubator number and shelf between: location: '&'2-Culture board: O\\'(l'I"L,.- Replicate number: 1 3 4 S I 6 7 I 8 I 9 10 YWTbatch: Culture board eu" number: ~ I 4. I. ..~

11. I,,,

\\';) I " 11.\\ 1.1-oS*0\\-'1.. Transfer vessel infonnation: I nH...,tS.U. Temnerature - 1.41;,. t 'c Selenastrum batch: CM*~()*n.. Average transfer volume (mL): O. CP.>'2.1...-lL Daily renewal information: Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 1 0 oil 2 O~ 10 - j 3 0&0"\\ 4 0&10 . 1 5 DC "'L '~ j 6 '11-7 as*I" "'1.- o.to'l - j

  • J Control iiJormation:

Acceptance criteria Summarv oftest endooints: % ofMale Adults: (\\7.

520%

7-day LCso ') 10..(O 0 % Adults havinQ 3" Broods: ....7 >80% NOEC 1000. % Mortalitv: A1

520%

LOEC \\.,""'" Mean OffsoriiNIFemale: .~\\.1 ~ 15.0 offspring/female ChV 1""<'." %CV: -~ \\'1. <40.0% IC" 10"'....1.1 I,dep..d,nl RovI..... !Iy I<elleye.Koon,n ~' SOP ATl4 - Exhibit ATl4.1, revision 06-01-11

Page 103 of 110 '1 J " J Page 2 of6 Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced n a a n () D 0 () () (J Adult mortality l-L. L.. L.. L.. L L.. L.. L-2 Young produced (') 0 .() () [) (l a 0 0 () Adult mortality L L. L.. l-L- L.. L.- L.- '-- 3 Young produced 0 0 D 0 0 () (') (). 0 () Adult mortality L L L. L. l-I...- l.- I.- 4 Young produced S '-\\ '-\\ S ~ s 5 '-\\ '-l '-\\ Adult mortality L I.- L. L L. I. L.. L. II.- 5 Young produced \\L.... 11-Ie 1\\ I~ 1\\ Ie \\\\ 1\\ IL Adult mortality L. \\......... l...- I.- L. \\-. \\- 6 Young produced D 0 0 0 0 D (] 0 0 0 Adult mortality L.. L L \\.. L I...- L I...- \\...... 7 Young produced 11 \\1 Ie::. 1'1" IS la IS Ii lie> II Total young produced

!l'-\\

33 '2.,,\\ 1.'\\ 31 !I~ ~b <33 3\\ 33 Final Adult Mortality L. l-l- \\.... I..... L. I...- \\........ X for 3rd Broods X )<- }<. ')(:.. )<. 'l'C )<.. 'Ie:: "1j '] '1, J Species: Ceriodaphnia dubia CONTROL CdNaClCR#: Survival andReproduction Data I~ Note: Adult mortality (L - hve, D = dead), SB - spltt brood (smgle brood split between two days), CO - carry over (offsprmg carried over with adult during transfer). D d S I dR NCIIL 600 Concentration: % Mortality: I D1. Mean Offspring/female: I,3\\.1 ID2 a urvlva an epro uctton ata Reulicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced f"\\ 0 0 (') () () n 0 () (") Adult mortality L L L. L L L L. L L L 2 Young produced U n n 0 () () 0 0 () 0 Adult mortality L L. L. L L. L L L L. 3 Young produced 0 0 () C) 0 C () 0 a n Adult mortality L L L I..... l.- I...... L L. L ~ 4 Young produced u,. "\\ S '-\\ '-\\ 5 '"\\ '"\\ "-I Adult mortality \\.- I.- L. I...- L' ..l... I...- L L 5 Young produced' \\1.. I"L lD It> 10 11-II ID \\1- /I Adult mortality '\\.... I...... I..... . I..... \\-. I...... 6 Young produced () 0 0 0 0 0 0 0 0 0 Adult mortality L L L \\.. L L. L L L I...- 7 Young produced 1'1 11.0 I' IS 18 Ii Ii II. II" .'\\ Total young produced 03 3"2.. ,be> 1.."1 31. 3"\\ a~ 3() 3~ 30 Final Adult Mortality l L L. \\- \\. \\- ~ J ~.J Note: Adult mortality (L - hve, D - dead), sa - spltt brood (smgle brood split between two days), CO - carry over (offspring carried over with adult during transfer). Concentration: % Mortality: 07. Mean Offspring/female: 031*., % Reduction from Control: 0.07. Ind.p,n~.n' RovlowOy KelleyE,Keen'n ~" SOP ATl4 - Exhibil ATl4.\\, revision 06-0\\-\\\\

Page 104 of 110 Page 3 of6 CdNaCICR#: ~ D d S I dR Species: CeriodaDhnia dubia 800 N CIIL mil a UTVlVa an epro uctzon ata Replicate number Dav 1 2 3 4 5 6 7 8 9 10 1 Young produced () t\\ () () () (") (") 0 0 Adult mortality L l L. L L L L. '- 2 Young produced 0 b 0 (:) () 0 0 a 0 () Adult mortality 1..- \\... L. L. L. L I,...... L. L.. 3 Young produced (", t> D 0 0 0 .(', 0 {) 0 Adult mortality \\- L. L L.- L. L.. 4 Young produced 50 " '-t S So U. '-t .c:. '-\\ '--\\ Adult mortality L. L. L. \\..- I.. \\.... L 5 Young produced 10 \\\\ \\..... 1\\ 11-ID Itl \\"Z.* IC> Adult mortality L. L. L. L L..... \\.... \\.... 6 Young produced 0 D 0 b () D () () 0 0 Adult mortality \\... L. L. L. L L. L. L..... 7 Young produced I,. II.:. 1"\\ I~ \\'-' \\" \\"b \\~ 110 I' Total young produced ~ ~'L '2.& ~'1.. ~'2. 3\\ "2.1 a~ ~'2... ~\\ Final Adult Mortalitv \\..... '- \\... L. L. °ETS 61 ~ "0 '.".('... i>:. .....:;1 ,)l':~",.:" ,T" !~' ~ En",onmem,ITostlng50Illllon..lnc. 1 ~, ~ J q I '-'- J Note. Adult mortahty (L - hve, D dead), sa spltt brood (smgle brood spltt between two days), CO carry over (offsprmg carried over with adult during transfer). Concentration: % Mortalitv: 07. Mean OffspringfFemale: 3\\. \\ % Reduction from Conirol: \\,"17.. d t' D t S I dR NCIIL 1000 mll: a UTVlva an epro uc zon aa Replicate number Day. 1 2 3 4 5 6 7 8 9 10 1 Young produced (') () () f\\ I"J a 0 () 0 t'i Adult mortality l-I,...... L L. L. l-I- I,...... L L.. 2 Young produced 0 () () {) 0 () f\\ 0 (') 0 Adult mortality \\..... L I,...... I,...... L.. L \\..... L.. L. L 3 Young produced 0 0 0 0 0 0 (\\ a CJ a Adult mortality I,...... \\ I L. L. L-L \\... L L.. 4 Young produced '-\\ '-I. 5:- 4 '-I. ~ \\..\\ \\.{ S Adult mortality I,...... \\..... \\.... L L. \\.... \\.... \\.... L.. 5 Young produced \\"1.- 10 \\\\ \\"b \\\\ ID \\'1. I"L ID 10 Adult mortality L.. L \\..... L L. L. L. L L. 6 Young produced (') /') (") () 0 (\\ [) 0 0 0 Adult mortality \\..... \\... L. L L. L L.. L \\.... L... 7 Young produced \\"\\ \\Ia 1'-4 IS I'" 1:'\\ I' I'; I¢ tC\\ Total young produced 3.\\ ~ ~C !l'Z, ~\\ 1.1 ~~ 3\\ 1.& ~O Final Adult Mortalitv \\. \\. L. L. ~ ~. I..- L.. Note: Adult mortality (L - hve, D:::: dead), SB - splIt brood (smgle brood split between two days), CO - carry over (offsprmg carried over with adult during transfer). lodepertder>t Rolli,wbV ](o1l'V~*KHn." loltl.,~ ~ Concentration: % Mortality: 01 Mean OffspringfFemale: 30, ~ % Reduction from Control: 0./.&:1'7. SOP AT14 - Exhibil ATl4.1, revision 06-01-11

Page 105 of 110 "ETS i~* - I,,; -~'~ "i'.* i ,;J, ~ in"","",.nl.llOllingSOIUllo""ro", Page 4 of6 CdNaCICR #: l~'i. d t' D t d t D t S 'I dR S 'I dR NCIIL Species: Ceriodaphnia dubia 1200 N CIIL Note: Adult mortality (L - hve, D - dead), SB - spltt brood (single brood splIt between two days), CO - carry over (offspnng carried over with adult during transfer). 1400 ml!: a urvlva an epro uc IOn aa Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 a n 0 5 () () (") 0 () Adult mortality L \\.... L.. L L-L.. L- ~ L- '- 2 Young produced 0 n 0 n 0 0 n 0 0 (') Adult mortality 'l.- L-L L-L- L L-L L 3 Young produced 0 0 (') 0 0 () n D () (") Adult mortality \\..- l-L L-L L \\. 4 Young produced C \\ \\ "L \\ b \\ \\ "L Adult mortality L.. L-L- L \\.... 5 Young produced '0 c:. "'2.. ~ ~ t> Cl .~ '-I \\ Adult mortality L \\ L L- \\... L. l 6 Young produced 0 D b D (;) S "L C 0 CJ Adult mortality \\... L. L... L 7 Young produced, \\Il' "\\ I., 5 1 "'\\ ~ 5 Total young produced '0 1"2., II 1,3 \\'Z., d Final Adult Mortality l- \\... Concentration: % Mortality: fJ7. Mean OffspringJFemale: ,\\:1 % Reduction from Control: 1A.<.t'. mil: a urvlVa an epro uc IOn aa Reolicate number Dav 1 2 3 4 5 6 7 8 9 10 1 Young produced () n () () n (') 0 t"'l (') a Adult ~ortality L L L L L L L L 2 Young produced ') ().. 0 0 (') (") 0 0 () (') Adult mortality L. L L L L L L-I- L-3 Young produced

J D

a 0 0 0 0 0 () () Adult mortality \\.- L L L l-I-. L. I-4 Young produced 0 0 \\ 0 \\ 0 \\ \\ ("') r.... Adult mortality L. L. L L... 5 Young produced () \\ 0 \\ 0 7-. (') C .,"2, Adult mortality L. L.. L.. I-. L. L \\... L \\..- 6 Young produced 0 0 f'\\ /") rJ (') () () C () Adult mortality \\.... L L L L L \\... L. 7 Young produced 5 ~ 0 ~ 4 I ,":I-0 0 "'Z...- Total young produced '5 '-l I 4 S 3 "l \\ ~ ~. Final Adult Mortalitv I.. \\. \\... \\. ~ ,J q I i.e]

  • J

.J '~ j

~ J Note. Adult mortality (L - live, D dead), sa spilt brood (smgle brood split between two days). CO - carry over (offspnng carried over with adult during transfer).

lodeFl<odont Ro,"ew~V K,lIovE.Ko,n,n fA.1S; Concentration: % Mortalitv: C7 Mean Offspring/Female: !J.<.t % Reduction from Control: &'l.~7_ SOP ATl4 - Exhibil ATl4.1, revision 06-01-11

Page 106 of 110 ~."'.'" T*******S.**.....

  • E.

77*: e '. ~

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..J Envlronme Verification of Ceriodaphnia Reproduction Totals 1000 mg NaCIIL

~ Day Re (icate number Total I 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 4 4 4 4 5 5 43 5 12 10 11 13 11 10 12 12 10 10 111 6 0 0 0 0 0 0 0 0 0 0 0 7 15 16 14 15 16 13 17 15 13 15 149 Total 31 30 30 32 31 27 33 31 28 30 303 Day Replicate Dumber Total I 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 5 6 5 5 4 4 4 46 5 12 12 10 11 10 11 10 11 11 12 110 6 0 0 0 0 0 0 0 0 0 0 0 7 17 17 15 13 15 18 15 18 16 17 161 Total 34 33 29 29 31 34 30 33 31 33 317 Control 1200 mg NaCIIL Day Replicate Dumber Total I 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 0 I I 2 I 3 3 I I 2 15 5 3 5 2 3 3 0 0 2 4 I 23 6 0 0 0 0 0 5 2 0 0 0 7 7 7 6 4 6 5 5 7 4 3 5 52 Total 10 12 7 11 9 13 12 7 8 8 97 600 mg NaCIIL ~ Day Replicate number Total I 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 4 5 4 4 6 4 44 5 12 12 10 10 10 12 11 10 12 11 110 6 0 0 0 0 0 0 0 0 0 0 0 7 17 16 15 15 18 17 18 16 16 15 163 Total 33 32 30 29 32 34 33 30 34 30 317 NaCIIL 1400 ~._u m~ Day Re licate Dumber Total I 2 3 4 5 6 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 0 0 I 0 I 0 I I 0 0 4 5 0 1 0 1 0 2 0 0 3 2 9 6 0 0 0 0 0 0 0 0 0 0 0 7 5 3 0 3 4 I 3 0 0 2 21 Total 5 4 I 4 5 3 4 I 3 4 34 800 mg NaCIIL ~ Day Re ticate number Total I 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 4 5 5 4 4 5 4 4 45 5 10 11 10 13 11 12 10 10 12 10 109 6 0 0 0 0 0 0 0 0 0 0 0 7 18 16 14 14 16 15 13 18 16 17 157 Total 33 32 28 32 32 31 27 33 32 31 311 File: CdNaCICR_050812.xlsx Entered by: J. Sumner Reviewed by:-Jf--

Page 107 of 110 (8!D,. ~H. I\\~rjl EYo ~ T 'I'"

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~ Environmental Testing Solutions, Inc. Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Qnality Control Verification ofData Entry, Calcnlations, and Statistical Analyses Test Dumber: CdNaCICR #134 Test dates: May 08-15, 2012 Concentration Renlicate number Survival Average reproduction Coefficient of Percent reduction from (mglL NaCl) I 2 3 4 8 (%) (offspring/female) variation (%) control ("k) 5 6 7 9 10 Control 34 33 29 29 31 34 30 33 31 33 100 31.7 6.1 Not applicable 600 33 32 30 29 32 34 33 30 34 30 100 31.7 5.8 0.0 800 33 32 28 32 32 31 27 33 32 31 100 31.1 6.5 1.9 1000 31 30 30 32 31 27 33 31 28 30 100 30.3 5.8 4.4 1200 10 12 7 II 9 13 12 7 8 8 100 9.7 22.8 69.4 1400 5 4 1 , 4 5 3 4 1 3 4 100 3.4 42.1 89.3 Dunnett's MSD value: PMSD: 1.927 6.1 MSD~ PMSD~ Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound detennined by USEPA (lalb percentile) = 13%. Upper PMSD bound detennined by USEPA (90th percentile) = 47%. Lower and upper PMSD bounds were detennined from the 10th and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 200Ib), USEPA, 20010, 2001b, Final Report: Interlaboratory Variability Study ofEPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix, EPA-821-B-OI-004 and EPA-821-B-OI-005, US Environmental Protection Agency, Cincinnati, OR. File: CdNaCICR_050812,xlsx Table populated from associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheet. Spreadsheet entered by: J. Sumner Reviewed by:--Jf--

Page 108 of 110 eElS

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"( ...:-:. Environmental Testing Soluth>ns, Inc. Statistical Analyses Cerlodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/8/2012 Test 10: CdNaCICR Sample 10: REF-Ref Toxicant End Date: 5/15/2012 lab 10: ETS-Envir. Testing Sol. Sample Type: NACl-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821~R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mQlL 1 2 3 4 5 6 7 8 9 1. D-Control 34.000 33.000 29.000 29.000 31.000 34.000 30.000 33.000 31.000 33.000 600 33.000 32.000 30.000 29.000 32.000 34.000 33.000 30.000 34.000 30.000 800 33.000 32.000 28.000 32.000 32.000 31.000 27.000 33.000 32.000 31.000 1000 31.000 30.000 30.000 32.000 31.000 27.000 33.000 31.000 28.000 30.000 1200 10.000 12.000 7.000 11.000 9.000 13.000 12.000 7.000 8.000 8.000 1400 5.000 4.000 1.000 4.000 5.000 3.000 4.000 1.000 3.000 4.000 Transform: Untransformed 1-Tailed Isotonic Cone-mall Mean N-Mean Mean Min Max CV% N t-Stat Critical MSo Mean N-Mean D-Control 31.700 1.0000 31.700 29.000 34.000 6.140 10 31.700 1.0000 600 31.700 1.0000 31.700 29.000 34.000 5.769 10 0.000 2.287 1.927 31.700 1.0000 800 31.100 0.9811 31.100 27.000 33.000 6.511 10 0.712 2.287 1.927 31.100 0.9811 1000 30.300 0.9558 30.300 27.000 33.000 5.832 10 1.661 2.287 1.927 30.300 0.9558 "'1200 9.700 0.3060 9.700 7.000 13.000 22.821 10 26.109 2.287 1.927 9.700 0.3060 "'1400 3.400 0.1073 3.400 1.000 5.000 42.054 10 33.586 2.287 1.927 3.400 0.1073 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 1.02293 1.035 -0.3894 -0.8256 Bartlett's Test indicates equal variances (p = 0.88) 1.79808 15.0863 Hypothesis Test (1-tail. 0.05) NoEC LOEC ChV TU MSDu MSDp MSS MSE F-Prob df Dunnett's Test 1000 1200 1095.45 1.92678 0.06078 1662.66 3.55 3.7E-43 5,54 Treatments vs D-Control 1500 500 1000 Dosemg/L 1.0.,------------, 0.7 0.8 0.9 =0.6 c8. 0.5 ~ 0.4 0.3 0.2 0.1 0.0...,~_~....F't::;::;....~~...j o Linear Interpolation (200 Resamples) 95%CL Skew 703.535 1009.92 -1.5251 1002.53 1024.94 -11.3848 1018.68 1040.35 -0.2299 1034.69 1055.49 -0.1685 1050.98 1071.19 -0.1022 1097.81 1118.78 0.0763 1129.3 1150.27 0.1443 91.4611 16.9162 5.71867 5.47513 5.29693 5.20953 5.52815 so mg/L 1001.8 1017.18 1032.57 1047.96 1063.35 1109.51 1140.29 Point IC05 IC10 IC15 IC20 IC25 IC40 IC50 Dose-Response Plot Ind'p."dont lloYfewbV "~'" 40 35 30 ~ 25 ,e20 &! 15 10 5 0 £i 0 0 0 0 0 0 0 0 0 0 c

=

N 8 r r C 1-tail,0.05Ievel of significance File: CdNaCICR_050812.xlsx Entered by: 1. s~ Reviewed bY:~.....

Page 109 of 110 I'1. I I Page 5 of6 Species: Ceriodaphnia dubia CdNaCICR #: \\~'-I SOP ATl4 - Exhibit ATl4.1, revision 06-01-11 Initial Jo6000 STOCK 1200 mg NaCIIL 1400 mg NaCIIL Concentration cJ ',,-1 J CONTROL (,,:!> c... J 8& , 1, u q U 600 mg NaCIIL r, - J L j ~ -~ 800 mg NaCIIL 1 c J 1000 mg NaCIIL

Page 110 of 110 0-1, j UETS ~ Environm,nlOlTosUng S"uri."~ Inc. Page 6 of6 Species: Ceriodaphnia dubia CdNaCICR #: I ~'-l Final Initial Final 1.~.'i Initial 2.~/.1 Final 1S.0 Initial Final Initial CONTROL 600 mg NaCIIL 800 mg NaCIIL 1000 mg NaCIIL 1200 mg NaCIIL 1400 mg NaCIIL -1 L 1 Concentration ~] r--------,-------=--------, 1 '- j ] ~ ] n

] I--__--+~~-+_=l.\\.~\\.g:__l~~~~~~~~......._:~~~~:_+_~~~~

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REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT - Mav 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 6/8/2012 CONCURRENCES Name R V C N Signature - Comment Date B.M. Love X U(~^Vl^p^ -^ (i/frl'Z. L.M. Koby X to /"[il^- J.W. Sabados X Q*-£i~ ^ s*db*A+/-^ c>l&llx-INSTRUCTIONS: Originatorwill determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.

S58 120712 800 - NPDES CORRESPONDENCE July 12, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L &C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR JUNE 2012 Enclosed is the June 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. In the Sequoyah Nuclear Plant Unit 2 Cycle 18 refueling outage of October 2012, TVA will replace the unit's four steam generators. During this large maintenance project, each of the steam generators will be cut free from existing piping, lifted out from the top of the concrete shield building, and maneuvered through the steel containment and internal structural concrete enclosures that house the steam generators. Cutting the temporary openings in the shield building and structural concrete enclosures using hydro-demolition will result in the generation of concrete rubble and hydro-demolition process waste water for disposal. Up to 1.5 million gallons of water supplied by SQN's existing Fire Protection System (Hixson Utility District potable water) will be used for hydro-demolition over a 7 day period. The hydro-demolition waste water will be filtered to 5 micron and the pH will be adjusted priorto flowing through SQN's normal waste water treatment process. As previously discussed with Bob Alexander with the Division, TVA does not believe the activity associated with replacing the steam generators will significantly change the nature or increase the quantity of pollutants discharged from permitted outfalls. SQN will continue to monitor Internal Monitoring Point 103 in accordance with NPDES permit TN0026450. Ifyou have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714.

Ms. Christina Morgan Page 2 July 12,2012 / certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. John iinjarnn Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D. Booker, MOB 1F-WBN B. E. Brickhouse, BR 4A-C J. T. Carlin, OPS 4A-SQN J. A. Cross, POB 2A-SQN T.R. Markum, BR 4A-C D. B. Nida, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 J.W. Proffitt, OPS 4C-SQN A. A. Ray, WT11A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN B. N. Smith (EDMS), MPB 1E-M

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 July 12, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L &C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR JUNE 2012 Enclosed is the June 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. In the Sequoyah Nuclear Plant Unit 2 Cycle 18 refueling outage of October 2012, TVA will replace the unit's four steam generators. During this large maintenance project, each of the steam generators will be cut free from existing piping, lifted out from the top of the concrete shield building, and maneuvered through the steel containment and internal structural concrete enclosures that house the steam generators. Cutting the temporary openings in the shield building and structural concrete enclosures using hydro-demolition will result in the generation of concrete rubble and hydro-demolition process waste water for disposal. Up to 1.5 million gallons of water supplied by SQN's existing Fire Protection System (Hixson Utility District potable water) will be used for hydro-demolition over a 7 day period. The hydro-demolition waste water will be filtered to 5 micron and the pH will be adjusted priorto flowing through SQN's normal waste water treatment process. As previously discussed with Bob Alexander with the Division, TVA does not believe the activity associated with replacing the steam generators will significantly change the nature or increase the quantity of pollutants discharged from permitted outfalls. SQN will continue to monitor Internal Monitoring Point 103 in accordance with NPDES permit TN0026450. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714.

Ms. Christina Morgan Page 2 July 12, 2012 / certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directlyresponsible for gathering the information, the information submittedis, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fineand imprisonment forknowing violations. ^President Sequoyah Nuclear Plant Enclosures cc (Enclosures): Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER From MAJOR (SUBR01) F - FINAL DIFFUSER DISCHARGE EFFLUENT

      • NO DISCHARGE

[ Form Approved. OMB No. 2040-0004 PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name _ JVA ^EQ^^HJ^CLE^R^I^NT jMdress__P.a_BOX2p00. aNJOEROFRCEOPSlN^QN) SODPX-DAISYJTN_37384 Facj!jty_ JA^-^QUOY^NUCLEAR PLANJ Lo<atiQEL Ji^lUPiLQQUNJY ATTN: Brad Love MONITORING PERIOD YEAR MO I DAY To YEAR MO DAY 12 06 I 01 12 06 30 NOTE: Read instructions before completing this form. PARAMETER TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 S EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT QUANTITY OR LOADING AVERAGE wwwwwwww ftftftftftftftft ftftftftftftftft MAXIMUM 1741 Req. Mon. DAILY MAX 2 DAILY MX UNITS 03 MGD 62 DEG C/HR MINIMUM NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penaltyoflawthatthisdocumentand ailattachmentswere preparedunder my direction or supervisioninaccordancewitha systemdesignedto assure thatqualified personnel properlygather and evaluatethe information submitted.Based on myinquiry ofthe person or persons who manage the system, or those persons directlyresponsible forgathering the information, the information submitted is, to the best of myknowledgeand belief,true, accurate, and complete. Iam aware that there are significantpenalties for submittingfalse information, includingthe possibilityoffine and imprisonmentfor knowingviolations. John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference allattachments here) No closed mode operation. Veliger monitoring data isincluded asan attachment. The following injections occurred: 1.Floguard MS6236 (max. calc. cone, was 0.059mg/L-limit 0.2mg/L) 2.Biodetergent 73551 (max. calc.cone,was 0.033mg/L-limit 2.0mg/L) 3. SpectrusCT1300 (max. calc. cone,was 0.037mg/L-limit 0.050mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used QUALITY OR CONCENTRATION AVERAGE 4t ft ft ft ft ft ft ft ftftftftftftftw 0.024 0.1 MOAVG MAXIMUM 41.0 Req. Mori. DAILY MAX 30.4 30.5 DAILY MX 3 DAILY MX 0.051 0.1 DAILY MAX UNITS 04 DEG.C. 04 DEG.C. 04 DEG.C. 19 MG/L NO. EX FREQUENCY OF ANALYSIS 30/30 CONTI NUOUS 30/30 CONTI NUOUS 30/30 CONTI NUOUS 30/30 CONTI NUOUS 26/30 FIVE PER WEEK 30/30 CONTI NUOUS SAMPLE TYPE RCORDR CALCTD MODELD CALCTD CALCTD CALCTD RCORDR RCORDR GRAB CALCTD CALCTD CALCTD TELEPHONE DATE Vice President OF PRINCIPAL EXECUTIVE OR AUTHORIZED AGENT 423 843-7001 AREA CODE NUMBER 12 07 YEAR MO Page 1 of 1 11 DAY

Sample Date Mean# of ZM/m3 % Settlers Water Temp. ('C) Sample Date Mean# of Asiatic Clams/m3 Water Temp. (°C) NOTES: % LOCATION SUB LOCATION Gravid Asiatic Clam COLLECTED BY 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 PB 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 PB 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW May 2012 No Samples Collected June 2012 No Samples Collected

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ JVA-Srai^WH_rW(^^F^I^OT ^djBSS_-P.a_BOX2000. aitreEQFRCEOPS^r^sQN) SODr^-J^ISYJlN_37384 Faaljty__ JVA.- SEQUOYi^NUCLEAR PLANJ J=2fiatLoji_JjJWi^ ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 101 T PERMIT NUMBER DISCHARGE NUMBER /IONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 06 01 12 06 30 MAJOR (SUBR01) F - FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT NO DISCHARGE Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completing this form. PARAMETER X QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT "ttHftfttltlHtlt Monitoring Not Required 23 PERCENT PERMIT REQUIREMENT w&ftftftftfttt / 43.2 MINIMUM £4fc4nfc&4?ft ,$tftftftwftft4t, SEMI ANNUAL COMPOS IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT ~' w4rwwwwww Monitoring Not Required '~ 23 PERCENT PERMIT REQUIREMENT iHkfc^HHHMfe wtWtWKMMWW 43.2 MIMINUM 4HkMHkA&& Aftft&ft ft ftft. SEMI ANNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEmTLE PRINCIPAL EXECUTIVE OFFICER John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED ICertify underpenaltyof lawthat thisdocumentand allattachmentswere prepared undermy directionor supervision inaccordance witha system designed to assure that qualifiedpersonnel propertygather and evaluate the information submitted.Based on myinquiryof the person or persons who manage the system, or those persons directlyresponsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significant penalties for submitting false information, including the possibilityof fine and imprisonmentfor knowingviolations. COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference all attachments here) Toxicity was not sampled in June 2012. EPA Form 3320-1 (REV 3/99) Previous editions may be used SIGNATORE'bF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE 423 843-7001 AREA CODE NUMBER DATE 12 07 YEAR MO Page 1 of 1 11 DAY

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 103 G PERMIT NUMBER DISCHARGE NUMBER From MAJOR (SUBR01) F - FINAL LOW VOL. WASTE TREATMENT POND EFFLUENT NO DISCHARGE [ Form Approved. OMB No. 2040-0004 PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name __'A^EQI^^HJ^C^^RPLANT ^di§s^^.O^BOX2000_ aNJEROmCEOPS^USQN) SODDYrDAISY1XN_37384 facmy_ JVA.r^QyOY^NUCLEAR PLANJ LocMoJl-Jd>^IIJPiLQOUJ£rY ATTN: Brad Love MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 06 01 12 06 30 NOTE: Read instructions before completing this form. PARAMETER PH 00400 1 0 EFFLUENT GROSS SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS OIL AND GREASE 00556 1 0 EFFLUENT GROSS FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT QUANTITY OR LOADING AVERAGE

0.966 Req. Mon. MOAVG MAXIMUM 1.231 Req. Mon DAILY MX UNITS 03 MGD MINIMUM 6 MINIMUM AftJfcftftft4fA> NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiryof the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. QUALITY OR CONCENTRATION AVERAGE 30 MOAVG <6 15 MOAVG MAXIMUM MAXIMUM 100 DAILY MX <6 20 DAILY MX Vice President UNITS 12 SU 19 MG/L 19 MG/L NO. EX TELEPHONE FREQUENCY OF ANALYSIS 14/30 THREE/ WEEK 2/30 TWICE/ MONTH 2/30 TWICE/ MONTH 30/30 SEE PERMIT SAMPLE TYPE GRAB GRAB GRAB GRAB GRAB GRAB RCORDR RCORDR DATE 423 843-7001 12 07 11 John T. Caiiin Sequoyah Site Vice President TYPED OR PRINTED SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OFANYVIOLATIONS (Reference allattachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name ___'A-SEQW^HJ^C^^RPLANT ^dr^^JPO^BOXJOOO. aNJEROFRCEOPSlN^QN) SODDY.- DAJSYJK37384 FacHjty_ J>^-^QUOY>^NUCLEAR PLANJ U(^on_JiAMiypiLQQUi£rY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE Form Approved. OMB No. 2040-0004 TN0026450 PERMIT NUMBER From 110 G DISCHARGE NUMBER XXl MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 06 01 12 06 30 NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. CENTIGRADE 00010 1 0 EFFLUENT GROSS VALUE TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 INSTREAM MONITORING TEMP. DIFF. BETWEEN SAMP. & UPSTRM DEG.C 00016 1 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL 50060 1 0 EFFLUENT GROSS VALUE TEMPERATURE - C, RATE OF CHANGE 82234 1 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT

vMnMnMntw Req. Mon. DAILY MX 2 DAILY MX 03 MGD 04 DEGC NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penaltyof lawthat this documentand allattachmentswere prepared under my directionor supervision inaccordance witha system designed to assure that qualifiedpersonnel properlygather and evaluate the information submitted. Based on myinquiryofthe person or persons who manage the system, or those persons directlyresponsible forgatheringthe information, the information submitted is, to the best of myknowledgeand belief,true, accurate, and complete. Iam aware that there are significantpenalties for submittingfalse information, including the possibilityof fine and imprisonmentfor knowing violations. John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OFANYVIOLATIONS (Reference allattachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used mWWttwtltWit 0.1 MOAVG REPORT DAILY MX 30.5 DAILY MX 5 DAILY MX 0.1 DAILY MX 04 DEGC 04 DEGC 04 DEGC 19 MG/L TELEPHONE Vice President 423 843-7001 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA CODE NUMBER CONTIN UOUS CONTIN UOUS CONTIN UOUS CONTIN UOUS Five per Week CONTIN UOUS CALCTD CALCTD CALCTD RCORDR CALCTD CALCTD DATE 12 07 11 YEAR MO DAY Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name _ JVA ^^^^^N^CL^RJ^LANT Address_.P.O^BOXgpOO. a^EQEOQEQE^riSQN) SOD^-DAjSYJlN^^ Facllity__T>^-SEf^^ LgratLon_ Ji>^IUPJiCOUNTY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 110 T PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 06 01 12 06 30 MAJOR (SUBR01) F - FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE XX Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 0 EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT AVERAGE MAXIMUM UNITS 'KlUtftWwtwtWt MINIMUM 43.2 MINIMUM 43*2 MINIMUM AVERAGE MAXIMUM UNITS 23 PERCENT 23 PERCENT SEMI ANNUAL SEMI ANNUAL COMPOS COMPOS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1Certifyunder penalty of lawthat this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the informationsubmitted. Based on my inquiryof the person or persons who manage the system, or those persons directlyresponsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. 1am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. [StoSoy^sr&Vt8e President TELEPHONE DATE John T. Carlin Sequoyah Site Vice President 423 843-7001 12 07 11 SIGlfatUR^OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED AREA CODE NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS No Discharge this Period (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different) Name _ JTVAJfEQ^^HJ^CLF^RJ^NT jMdr^s__P.a_BOX2000. (LNIPROFFiQE OPS£NJ>QN) SQDr^-JMSYJ^7^ FacHjty_ _TVA-SEQUOY^NUCLEAR PLANJ JLsi^on_Ji>^IUPJLQQyi£TY ATTN: Brad Love NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) From TN0026450 PERMIT NUMBER 118 G DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY To YEAR MO DAY 12 06 01 12 06 30 MAJOR (SUBR01) F - FINAL WASTEWATER & STORM WATER EFFLUENT NO DISCHARGE XX Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE OXYGEN, DISSOLVED (DO) 00300 1 0 EFFLUENT GROSS SOLIDS, TOTAL SUSPENDED 00530 1 0 EFFLUENT GROSS SOLIDS, SETTLEABLE 00545 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT AVERAGE MAXIMUM UNITS MINIMUM 2 MINIMUM Aftftftftftftft AVERAGE "kitMHkftft)Nt IHtWKwtltlUt MAXIMUM 100 DAILY MX UNITS 19 MG/L 19 MG/L 25 MUL TWICE/ WEEK TWICE/ WEEK GRAB GRAB GRAB PERMIT REQUIREMENT DAILY MX ONCE/ MONTH FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1 0 EFFLUENT GROSS SAMPLE MEASUREMENT 03 MGD WWWKWRXW PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT Req. Mon. MOAVG Req. Mon. DAILY MX NAMEHTTLE PRINCIPAL EXECUTIVE OFFICER Certify under penalty of lawthat this document and allattachments were prepared under my directionor supervision in accordance witha system designed to assure that qualifiedpersonnel properly gather and evaluate the information submitted. Based on my inquiryof the person or persons who manage the system, or those persons directlyresponsible forgatheringthe information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. John T. Carlin Sequoyah Site Vice President TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the DredgePondotherthan that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used wtWWWWWmW SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE 423 843-7001 AREA CODE NUMBER ONCE/ BATCH ESTIMA DATE 12 07 11 YEAR MO DAY Page 1 of 1

REVIEW/CONCURRENCE SHEET DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT - June 2012 DMR ORGANIZATION: Environmental DOCUMENT PREPARED BY: Brad Love DATE: 7/10/2012 CONCURRENCES Name R V C N Signature - Comment Date B.M. Love X %Gf^ ^p?^ 7/<<*/'£ L.M. Koby X 7/<<fi^ J.A. Cross X >f/jAJU*U{/i(P<> 1 INSTRUCTIONS: Originator will determine the review/concurrence assignment. REVIEW: Examine technical content and commitments made. A review (RV) should confirm the truth and accuracy of factual statements and indicate agreement with commitments made which are applicable to the reviewer's organization. CONCURRENCE: Indication of agreement with the document as a whole. Concurrence (CN) signifies that the document is responsive to the intended purpose, logical in construction, and clear in meaning in the eyes of the recipient. A concurrence signature indicates that the individual would be willing to sign the document for the agency.}}

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