ML20206G345

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1998 Annual Environ Rept,Non-Radiological
ML20206G345
Person / Time
Site: Beaver Valley
Issue date: 12/31/1998
From:
DUQUESNE LIGHT CO.
To:
Shared Package
ML20206G332 List:
References
NUDOCS 9905070179
Download: ML20206G345 (84)


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I l 1998 ANNUAL ENVIRONMENTAL REPORT NON-RADIOLOGICAL DUQUESNE LIGHT COMPANY BEAVER VALLEY POWER STATION UNITS NO.1 AND 2 LICENSES DPR-66 AND NPF-73 l l

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TABLE OF CONTENTS LIST OF TABLES .

        - LIST OF FIGURES Page 1

EXEC UTIVE SU M MARY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ES-1 1 I NTROD U Ci lON . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1 1.1 Ob,'ectives of the Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1 i 1.2 Scope of Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1 1.2.1 Benthic Macroinvertebrate Monitoring . . . . . . . . . . . . . . . . . . . . . . 1-2 1.2.2 Fish M onitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2 1.2.3 Larval Cages / Zebra Mussel Scraper / Bridal Vell Samplers / Pump /Blobox Sampling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2 1.2.4 Corbicula/ Zebra Mussel Density Determination s . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3 1.2.5 Monthly Activity Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3 1.3 Site De scription . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4 2 AQUATIC MONITORING PROGR AM . . . -. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . '2-1 2.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1 2.2 Benthos.................................................... 2-1 2.2.1 Obje ctive s . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1 2.2.2 M e th o d s . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1 2.2.3 ' H abitats . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2 1 2.2.4 R e s u lts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2 2.2.5 Community Structure and Spatial Distribution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3 2.2.6 Comparison of Control and Non-Control S ta tio n s . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 2.2.7 Seasonal Comparison . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4 2.2.8 Discu s sion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4 2.3 Fish....................................................... 2-5 2.3.1 O bjective . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5 2.3.2 M eth o d s . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5 2.3.3 R e s ults . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6 2.3.4 Comparison of Control and Non-Control Stations . . . . . . . . . . . . 2-8 2.3.5 Di s cu s sion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8 2.4 Corbicula Monitoring Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9 2.4.1 Introdu ction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9 2.4.2 M o nitori n g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9 2.4.3 Corbicula Larvae Study . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-12 2.5 Zebra Mussel Monitoring Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14 2.5.1 - Introduction ' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14 2.5.2 M o nitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15 3 R EF ER E N C ES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

                                                       -LIST OF TABLES -

Table No. Title 2.1 Duquesne Light Company BVPS ~ Sampling Dates for 1998 2.2 Systematic List of Macroinvertebrates Collected From 1973 Through 1998

                              - in the Ohio River Near BVPS (6 sheets) 2.3           Benthic Macroinvertebrate Counts For Triplicate Samples Taken at Each Sample Station By Sample Date for 1998 (4 sheets) '

2.4 - 'Mean Number of Macroinvertebrates (Numb'er/m') and Percent Composition of Oligochaeta, Chironomidae, Mollusca and Other Organisms,1998 - BVPS 2.5 Mean Number of Macroinvertebrates (Number /m') and Percent Composition of Oligochseta, Chironomidae, Mollusca, and Other Organisms for the Control 1 Station (1) and the Average for Non-Control Stations (2A,281,2B2,283, and 3),1998 i BVPS I 2.6 , Shannon-Weiner Diversity, Evenness and Richness indices for Benthic. i

                              , Macroinvertebrates Collected in the Ohio River,1998                                     1 2

2.7 Benthic Macroinvertebrate Densities (Number /m ) for Station 1 (Control) and Station 2B (Non-Control) During Preoperational and Operational Years Through 1998 l BVPS (2 sheets) J 2.8 Scientific and Common Name of Fish Collected in the New Cumberland Poo! of the Ohio River,1970 through 1998, BVPS (3 sheets)

2.9 Comparison of Control Vs. Non-Control Electrofishing Catches  ;

During the BVPS 1998 Fisheries Survey ) 2.10 Comparison of Control Vs. Non-Control Seine Catches During the BVPS 1998 Fisheries Survey 2.11' Fish Species Collected During the May 1998 Sampiing of the Ohio Riverin the Vicinity of BVPS 2.12 Fish Species Collected During the July 1998 Sampling 'I' of the Ohio River in the Vicinity of the BVPS 2.13 Fish Species Collected During the September 1998 Sampling of the Ohio Riverin the Vicinity of the BVPS 2.14 Fish Species Collected During the November 1998 Sampling of the Ohio River in the Vicinity of the BVPS

I l LIST OF TABLES (Cont'd) ) Table No. Title 2.15 Estimated Number of Fish Observed During Electrofishing Operations 2.16 Catch Per Unit of Effort (CPUE as Fish /Electrofishing Minute) By Season During the BVPS 1996 Fisheries Survey (2 sheets) 2.17 Catch Per Unit of Effort (CPUE as Fish /Electrofishing Minute) By Season During the BVPS 1997 Fisheries Survey (3 sheets) 2.18 Catch Per Unit of Effort (CPUE as Fish /Electrofishing Minute) By Season During the BVPS 1998 Fisheries Survey (2 sheets) 2.19 Unit 1 Cooling Reservoir Monthly Sampling Corbicula Density Data for 1998 from BVPS 2.20 Unit 2 Cooling Reservoir Monthly Sampling Corbicula Density Data for 1998 from BVPS 2.21 Estimated Density of Zebra Mussel Veligers (Number /m') in Pump Samples from the BVPS Barge Slip, Water intake Structure, Emergency Outfall Building, Splash Pool, Cooling Tower Unit #1, and Cool;ng Tower Unit #2 for 1998 (2 sheets) 2.22 Number of Observed Zebra Mussels in Clam Cage and Ponar Dredge Samples From the intake Structure Sample Locations A and D at BVPS for 1998 l l

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i ..a l LIST OF FIGURES i Figure No. Title 1.1 Location Map for the 1998 Beaver Valley Power Station Aquatic Monitoring Program l Sampling Control and Non-Control Sampling Stations 1.2 Location Map for Beaver Valley Power Station Benthic Organism Survey i Sampling Sites for the 1998 Study  ; 1.3 Location Map for Beaver Valley Power Station Fish Population Survey Fish Sampling Sites for the 1998 Study 1.4 Location of Study Area, Beaver Valley Power Station Shippingport, Pennsylvania BVPS 2.1 Comparison of Live Corbicula Clam Density Estimates Among 1998 BVPS Unit 1 Cooling Tower Reservoir Sample Events, ForVarious Clam Shell Size Groups 2.2 Comparison of Live Corbicula Clam Density Estimates Among 1998 BVPS Unit 2 Cooling Tower Reservoir Sample Events, ForVarious Clam Shell Size Groups 2.3 Comparison of Live Corbicula Clam Density Estimates Among 1998 Intake Structure Sample Events, For Various Clam Shell Size Groups 2.4 Water Temperature and River Elevation Recorded at the Ohio River at BVPS Intake Structure During the 1998 Monthly Sampling

., 1 ES-1
EXECUTIVE

SUMMARY

The 1998 Beaver Valley Power Station (BVPS) Units 1 and 2 Non-Radiological Environmental Monitoring Program consisted of an Aquatic Program that included surveillance and field sampling of Ohio River aquatic life. The Aquatic Program is an annual program conducted by Duquesne Light Company to provide baseline aquatic resources data, to assess the impact of the operation of BVPS on the aquatic ecosystem of the Ohio River, and to monitor for potential J impacts of biofouling organisms (Corbicula and zebra mussels) on BVPS operations. This is the 22nd year of operational environmental monitoring for Unit 1 and the 10th for Unit 2. As in ] previous years, the results of the program did not indicate any adverse environmental impact to the aquatic life in the Ohio River associated with the operation of BVPS. The 1998 benthic macroinvertebrate surveys did not indicate any abnormal community structure l for the Ohio River either upstream or downstream from BVPS. These benthic surveys are a continuation of a Fate and Effects Study (1990 through 1992) conducted for the Pennsylvania Department of Environmental Protection to assess ecosystem impacts of the molluscicide CT-1 l that is used to control biofouling organisms at BVPS. To date, these studies do not indicate that j

        . the continued use of CT-1 at the BVPS has been detrimental to the aquatic community below              !

the BVPS discharge. Substrate was probably the most important factorinfluencing the distribution and abundance of the benthic macroinvertebrates in the Ohio River near BVPS. Soft muck-type substrates along the shoreline were conducive to worm and midge proliferation, while limiting macroinvertebrates that require a more stable bottom. Oligochaeta (segmented worms) accounted for 55 percent of the macrobenthos collected in 1998, whereas Chironomidae (midge fly) and Mollusca (snails and bivalves) accounted for about 27 percent and 12 percent, respectively, in 1998,12 species were added to the cumulative taxa list of macroinvertebrates collected near BVPS. Community structure has changed little since pre-operational years, and program results did not indicate that BVPS operations were affecting the benthic community of the Ohio River. , The fish community of the Ohio River in the vicinity of BVPS was sampled in 1998 by night electrofishing and daytime seining. Results forthe 1998 fish surveys indicate normal community structure for the Ohio River based on species composition and relative abundance. Since j monitoring began in the early seventies, the number of identified fish taxa has increased from , 43 to 77 for the New Cumberland Pool. l

        'During the 1998 survey, forage species were collected in the highest numbers, particularly gizzard shad and emerald shiners. This indicates a healthy fish community, since game species
(predators) rely on this forage base for their survival. Yearly variations in total annual catch are

l ES-2 an expected occurrence and are attributable primarily to fluctuations in th'e population size of the forage species and naturally-occurring environmental changes. Forage species, such as 1 gizzard shad and emerald shiner with high reproductive potentials frequently respond to changes in natural environmental factors (competition, food availability, cover, and water quality) . with large fluctuations in population size that subsequently affect the predators that depend on them. Although variations in total catch occurred from station to station in 1998, species composition remained fairiy stable. Common taxa collected in the 1998 surveys by all methods included gizzard shad, emerald shiner, mimic shiner, redhorse species, channel catfish, buffalo species, 4 common carp, sauger, freshwater drum, quillback, and smallmouth bass. Little difference was observed in species composition of the catch between the Control (1) and Non-Control Stations (2A,2B, and 3). A greater number of fish representing more species were captured at non-control stations than control stations, but this was most likely due to the extra effort expended j at non-control stations versus control stations. Habitat preference is probably the most influential factor that affects where and in what relative abundance the different species of fish are collected. .' At the request of the Pennsylvania Fish and Boat Commission (PAF&BC), electrofishing catch j rates were calculated as fish per minute (i.e., power on time) of sampling for data from the 1996 through 1998 sample years. The annual electrofishing rate for 1998 was 2.3 times lower than in 1997 and 1.6 times greater than in 1996. Differences among months and years in electrofishing catch rates were probably due to a combination of the number and species of fish present and environmental conditions (e.g., turbidity and flow) on the sampling dates that affect the effectiveness of the electrofishing techniques The monthly reservoir scraper samples collected in Units 1 and 2 cooling towers during 1998 indicated Corbicula were entering and color:lzing the reservoirs. The monthly clam density estimates for Unit 1 indicated a low rate of colonization had occurred prior to May when one live Corbicula was found. Later in the season, September through October, Corbicula were collected in high numbers. One live Corbicula was collected in November. Data from Unit 2 indicate that one live Corbicula was collected in November. No sediment samples were collected in the Unit 1 or Unit 2 cooling towers during the scheduled outages. . I Since 1991, zebra mussels have been moving progressively upstream in the Ohio River. In 1993, zebra mussels were identified at the Pike Island Locks and Dam (mile point 84.2),50 miles downstream of BVPS. In 1994, zebra mussels were identified in the Ohio River upstream j from the BVPS at the Emsworth Locks and Dam (mile point 6.2) and at Lock and Dam 4 and 7 l on the Allegheny River. The U.S. Army Corps of Engineers reported zebra mussels at the New 1

i ES-3 Cumberland Locks and Dam (Ohio River) on May 11,1995 and on July 28,1995,16 zebra mussels were repor1ed at the Maxwell Locks and Dam (Monongahela River). In 1995 and 1996, live zebra mussels were found by divers in the BVPS main intake structure and auxiliary intake structure during scheduled cleaning operations. During 1997, zebra musselveligers, juveniles, and adults were observed in sample collections for the first time. Veligers were observed in June and September 1997. J uvenile mussels were collected in August, October, and November 1997. Zebra mussels were detected in the pump and substrate samples collected during 1998. Zebra mussel veligers were detected in various pump samples from August through November. Juvenile mussels were observed in clam cage and ponar dredge samples from intake Structures A and D at BVPS. The observation of adult zebra mussels in the intake ponar dredge samples in November 1997 as well as one in the Unit 2 cooling tower reservoir in November 1998 confirms previous observations by divers that zebra mussels are colonizing the area in and around the BVPS intake structure. To more fully document zebra musselinfestation at BVPS, additional sampling was initiated in 1998 to determine the extent and severity ofinfestation of service water. This additional sampling included placement of an in-line blomonitor at the impact basin below the emergency outfall to monitor settlement and additional pump sampling at this location to 1 determine the density of veligers in the plant's raw water system. I I i

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1-1 l 1 INTRODUCTION i This report summarizes the Non-Radiological Environmental Program conducted by Duquesne Light Company (DLC) during 1998, for the Beaver Valley Power Station (BVPS) Units 1 and 2;

 - Operating License Numbers DPR-66 and NPF-73. This is a voluntary program, because on February 26,1980, the Nuclear Regulatory Commission (N RC) granted DLC's request to delete all of the Aquatic Monitoring Program, with the exception of the fish impingement program (Amendment No. 25), from the Environmentel Technical Specifications (ETS). In 1983, DLC was permitted to also delete the fish impingement studies from the ETS program of required sampling along with non-radiological water quality requirements. However, in the interest of providing an uninterrupted database, DLC is continuing the Aquatic Monitoring Program.

1.1 Objectives of the Program The objectives of the 1998 environmental program were: (1) To monitor for any possible environmentalimpact of BVPS operation on the benthic macroinvertebrate and fish communities in the Ohio River; (2) To provide a minimal sampling program for continuing an uninterrupted environmental database for the Ohio River near BVPS, pre-operational to present; and (3) To evaluate the presence, growth, and reproduction of macrofouling Corbicula (Asiatic

           ' clam) and zebra mussels (Dreissena app.) at BVPS.

1.2 Scope of Services Acres performed the 1998 Aquatic Monitoring Program as specified in the Environmental Programs Manual Procedure (EPMP) 5.01 - Aquatic Ecological Monitoring Procedures. This EPMP describes in detail the field and laboratory procedures used in the various monitoring programs, as well as the data analysis and reporting requirements. These procedures are summarized according to task below.

1-2 l I 1.2.1 Benthic Macroinvertebrate Monitoring The benthic macroinvertebrate monitoring program consisted of benthic sampling by a { ponargrab sampler at four stations on the Ohio River. Prior to 1996, duplicate sampling I occurred at Stations 1,2A, and 3, while triplicate sampling occurred at Station 2B (i.e., one sample at each shoreline and mid-channel)(Figures 1.1 and 1.2). In 1996, a review I of the sampling design suggested that sampling should be performed in triplicate at each station to conform with standardized USEPA procedures. Therefore, starting in l 1996, triplicate samples were taken at Stations 1,2A, and 3, as in 1995, with triplicate samples also collected at each shore and mid-channel location at Station 2B. A petite

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( ponar was used to collect the samples, replacing the standard ponar used in prior -} studies. This sampling was conducted in May and September,1998. For each field { effort, a total of 18 benthic . samples was collected and processed in the laboratory, as described in the EPMP. 1.2.2 Fish Monitoring The fish monitoring program consisted of seasonal sampling (May, July, September, and November) using two techniques: boat electrofishing and seining. Boat electrofishing was conducted at night at Stations 1,2A,28, and 3 (both shorelines) (Figure 1.3). Seining occurred at Stations 1 and 28 during the day and generally in early evening. All field procedures and data analysis were conducted in accordance with the EPMP. 1.2.3 Larval Cages / Zebra Mussel Scraper / Bridal Vell Samplers / ' Pump /Biobox Sampling I Larval cages (two long term and two short term) were set in the project intake structure to sample for Corbicula in 1996. The cages continued to monitor for Corticula through August 1997. Results from Acres study in April through June 1997 to compare short-term larval cages to ponar samples indicated ponar samples provided comparable resuits to short-term larval cages for monthly sampling. In August 1997, ponar sampling replaced short-term larval cage sampling. Long-term cages remained in until May 1998 when all larval cages were removed at the request of BVPS personnel. Wall scraping samples were collected monthly from the Unit 1 cooling tower, the Unit 2 cooling tower, the barge slip, and the intake wall in 1996 and 1997. Wall scrapings were taken with a D-frame scraper, with five scrapes of approximately 2 ft each made per sample at the sampling locations, in 1998, two additionallocations were added; the

l 1-3 ) emergency.outfall basin (June through Novembar) and the impact basin (August through November).  !

                                                                                               'I Bridal veil samplers were deployed at the intake structure in 1996 to monitor for zebra mussel veligers. The samplers were removed, and the bridal veil replaced and                    !

redeployed once per month until May 1998 when the samplers were removed at the

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request of BVPS personnel. j l 1 The intake sampling and wall scraping sampling was historically conducted once per j month, year long. Beginning in December 1997, it was decided to forego sampling in December and January of each year, since buildup of the target organisms, Corbicula i and zebra mussels, does not occur in these cold water months. Monthly sampling is maintained throughout the balance of the year. l A pump sample was collected at the barge slip location for zebra mussel veligers j monthly from April through Octoberin 1996 and 1997. The scope of the sampling was expanded in 1998 to include the intake structure as well as the barge slip, in June 1998,

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I the emergency outfall basin and splash pool locations were also added. Additional pump samples were collected from the cooling tower of Unit i and Unit 2 in October. j I At the request of DLC, sampling was extended through November in 1998. in April 1998, a blobox was set up at the emergency outfall basin to monitor for settling zebra mussels. The biobox was checked each month, and four substrate plates were removed and analyzed in November 1998. 1.2.4 Corbicula/ Zebra Mussel Density Determinations During 1998, Acres was not required to make any collections from the Unit 1 and Unit 2 cooling tower reservoirs because of extended unit outages. During all Corbicula/ zebra mussel sampling activities (Tasks 3 and 4), observations were made of the shoreline and other adjoining hard substrates for the presence of macrofouling species. j l 1.2.5 Monthly Activity Repods i Activity reports were prepared each month that summarized the activities that took place the previous month. The reports included the results of the monthly Corbicula/ zebra mussel monitoring including any trends observed and arr preliminary results available

4 l 1-4 from the benthic and fisheries programs. The reports addressed progress made on I each task, and reported any observed biological activity of interest. 1 1.3 Site Description BVPS is located on a 501-acre tract oflanc on the south bank of the Ohio Riverin the Borough of Shippingport, Beaver County, Pennsylvania. The Shippingport Atomic Power Station once shared the site with BVPS before being decommissioned. Figure 1.4 is a plan view of BVPS. The site is appr-Amstely 1 mile (1.6 km) from Midland, Pennsylvania; 5 miles (8 km) from East Liverpool, Ohio; and 25 miles (40 km) from Pittsburgh, Pennsylvania. The population within a 5 mile (8 km) radius of the plant is approximately 18,000. The Borough of Midland, Pennsylvania has a population of approximately 3,500. The site lies along the Ohio River in a valley which has a gradual slope extending from the river (elevation 665 ft. (203 m) above sea level) to an olevation of 1,160 ft (354 m) along a ridge south of BVPS. Plant entrance elevation at the station is approximately 735 ft (224 m) above sea level. The station is situated on the Ohio River at river mile 34.8, at a location on the New Cumberland Pool that is 3.3 river miles (5.3 km) downstream from Montgomery Lock and Dam and 19.4 miles (31.2 km) upstream from New Cumberland Lock and Dam (Latitude: 40*, 36',18"; Longitude: 80 *, 26', 02"). The Pennsylvania-Ohio-West Virginia borderis 5.2 river miles (8.4 km) downstream from the site. The river flow is regulated by a series of dams and reservoirs on the Beaver, Allegheny, Monongahela, and Ohio Rivers and their tributaries.

      ' Ohio River water temperatures generally vary from 32' to 84*F (O' to 2g*C). Minimum and maximum temperatures generally occur in January and July / August, respectively, i

BVPS Units 1 and 2 have a thermal rating of 2,660 megawatts (MW). Units 1 & 2 have a design electrical rating of 835 MW and 836 MW, respectively. The circulating water systems are a closed cycle system using a cooling tower to minimize heat released to the Ohio River. Commercial operation of BVPS Unit 1 began in 1976 and Unit 2 began operation in 1987. l l L l

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2-1 2 AQUATIC MONITORING PROGRAM 2.1. Introduction The environmental study area, established to assess potentialimpacts, consists of four sampling stations each having a north and south shore (Figure 1.1). Station 1 is located at river mile (RM) 34.5, approximately 0.3 mile (0.5 km) upstream of BVPS and is the control station. Station 2A is located approximately 0.5 mile (0.8 km) downstream of the BVPS discharge structure in the q main channel. Station 2B is located in the back channel of Phillis Island, also 0.5 mile downstream of the BVPS discharge structure. Station 2B is the principal non-control station because the majority of discharges from BVPS Units 1 and 2 are released to the back channel. Station 3 is located approximately 2 miles (3.2 km) downstream of BVPS. Sampling dates for each of the program elements are presented in Table 2.1. The following sections summarize the findings for each of the program elements. 2.2 Benthos 2.2.1 Objectives The objectives of the benthic surveys were to characterize the benthic macroinvertebrates of the Ohio River near BVPS and to determine the impacts,if any, of BVPS operations. 2.2.2 Methods Benthic surveys were scheduled and performed in May and September,1998. Benthos samples were collected at Stations 1,2A,2B, and 3 (Figure 1.2), using a petite ponar grab sampler. Triplicate samples were taken off the south shore at Stations 1,2A, and

3. Sampling at Station 2B, in the back channel of Phillis Island, consisted of triplicate petite ponar grabs at the south side, middle, and north side of the channel (i.e., sample Stations 2B1,282, and 2B3, respectively).

The contents of each grab were gently washed through a U.S. Standard No. 30 sieve and the retained contents were placed in a bottle and preserved in ethanol. In the laboratory, rose bengal stain was added to aid in sorting and identifying the benthic organisms. Macroinvertebrates were sorted from each sample, identified to the lowest

                                                                                                     ~

2-2 taxon practical and counted. Mean densities (number /m') for each taxon were calculated for each replicate. Four species diversity indices were calculated: Shannon. Weiner; evenness (Pielou,1969), species richness, and the number of taxa. These estimates provide an indication of the relative quality of the macroinvertebrate community. 2.2.3 Habitats i Substrate type is an important factor in determining the composition of the benthic community. Two distinct benthic habitats existed in the Ohio River near BVPS. These habitats are the result of damming, channelization, and river traffic. Shoreline habitats were generally soft muck substrates composed of sand, silt, and detritus. An exception occurred along the north shoreline of Phillis Island at Station 2A where clay and sand dominated. The other distinct habitat, hard substrate, was located at mid-tiver. The hard substrate is probably the result of channelization and scouring by river currents and turbulence from commercial boat traffic. 2.2.4 Results i Fifty-seven macroinvertebrate taxa were identified during the 1998 monitoring program (Tables 2.2 and 2.3). There were 8,380 macroinvertebrates/m2 collected in May and 16,055/m$ in September (Table 2.4). The macroinvertebrate assemblage during 1998 was dominated by burrowing organisms typical of soft unconsolidated substrates. Oligochaetes (segmented worms) and chironomid (midge fly) larvae were abundant (Table 2.4). Fourteen of the 57 taxa were presentin both May and September. Twenty taxa present in the May samples that were not in the September samples, and 23 taxa in the September samples, were not present in the May samples (Table 2.3). The Asiatic clam (Corbicula ag), has been observed in the Ohio River near BVPS from , 1974 to present. Zebra mussels were collected in the BVPS benthic samples in 1998. Adult zebra mussels, however, were detected in 1995 and 1996 by divers in the BVPS main and auxiliary intake structures during scheduled cleaning operations. ' Zebra  ; mussel veligers, adults andjuveniles were collected during the 1997 sampling program. Zebra mussel veligers were again detected in pump samples taken in 1998, juveniles a were present in the intake structure samples, and an adult was present in the scraping  ! sample taken at the Unit 2 cooling tower reservoir (see Section 2.5, Zebra Mussel l Monitoring Program). )

2-3 in 1998,12 taxa were added to the cumulative taxa list of macroinvertebrates collected near BVPS (Table 2.2). These included one genus of segmented worm, two genera of mayfly, two genera of caddisfly, one family of beetle (although genera within this family have been previously found); four genera of midgefly, one genus of biting midge and one superfamily of bivalve (although genera within this superfamily have also been found). No state or Federal threatened or endangered macroinvertebtsie species were collected during 1998. 2.2.6 Community Structure and Spatial Distribution Oligochaetes accounted for the highest mean density of macroinvertebrates in May and September 1998 (5,617/m2 and 7,834/m 2, respectively). Chironomids had the second 2 highest mean density in both May 1998 (1,641/m ) and September 1998 (5,098/m2). Station 3 had the highest mean density of macroinvertebrates with a total of 2,606

  • organisms /m2 in May and 11,462/m2in September. Station 2B2 had the lowest mean density of organisms in May (389/m2) and September (57/m').

2.2.6 Comparison of Control and Non-Control Stations For this analysis, Station 1 is designated the control station since it is always out of the influence of the BVPS discharge and Station 2B the non-control station since it is the station subjected most to the 1998 discharge.' Stations 3 and 2A may be under the influence of the plume under certain conditions, but it is unlikely that they are regularly influenced by BVPS. Species composition .between the control and non-control sample stations was comparable in May (Table 2.5). The density of macroinvertebrates found at the control

 - station was two times greater than at the non-control station, however. The density of oligochaetes was much higher at the control station (1,210/m 2) than at the non-control station (374/m8 ). Chironomids were also present at greater densities at the control station (446/m') than at the non-control station (288/m') These differences probably reflect the differences in substrate between the stations rather than project-related impacts. The control station substrate was dominated by soft sediments which are preferred by these organisms while coarser substrate was common at the non-control station.

In September, approximately 4.5 times more macroinvertebrates were present at the control than at the non-control station. The dominant organisms at the control station were chironomids and mollusks (generally Corbicula), which were present at densities 2 2 of 994/m and 965/m , respectively. Chironomids were also the dominant organism at

2-4 the non-control station (283/m'), however, at a lower density than at the control station. i As in May, the differences observed between Station 1 (control) and Station 2B (non-control) were probably related to observed differences in habitat at each station. Differences were within the expected range of variation for natural populations of macroinvertebrates. Indices were calculated to determine the relative diversity, evenness, and richness of the macroinvertebrate population structure among stations and between control and non-control sites. The Shannon-Weinerdiversity indices in May collections ranged from 1.32 at Station 2A to 2.74 at Station 2B3 (Table 2.6). A higher diversity index indicates a relatively "better" structured assemblage of organisms, while a lower index generally indicates a low quality or stressed community. Evenness, an index that estimates the relative contribution of each taxon to the community assemblage, ranged from 0.20 at Station 2A to 0.51 at control Station 2B2. The community richness, another estimate of the quality of the macroinvertebrate community, was greatest at Station 283 and lowest at Station 2A. I l In September, the highest diversity was present at control Station 1 and lowest at non control Station 282. Evenness ranged from 0.28 at Station 3 to 1.00 at Station 282. Richness was greatest at Station 1 and lowest at Station 2B3. The low number of taxa (four) and total organisms (four) collected at Station 2B2 was probably a result of the substrate present, which was a coarse, hard substrate that ponar techniques under-sample, rather than a result of any effect of the BVPS discharge. These low numbers tend to lower the reliability of these indices. No impacts of the BVPS on the benthic community, as measured by differences between control and non-control stations, were evident. I 2.2.7. Seasonal Comparison The density of benthic organisms observed in September 1998 was about two times greater than in May 1998 (Table 2.3). Thirty-four taxa were collected in May, and 37 ,, taxa were collected in September. Oligochaetes were the most commonly collected i macroinvertebrates. Chironomids and mollusks were also common in both the May and September samples. 2.2.8 Discussion Substrate was probably the most important factor controlling the distribution and

    ' abundance of the benthic macroinvertebrates in the Ohio River near BVPS. Soft, mucky substrates that existed along the shoreline are conducive to oligochaete, chironomid, and mollusk proliferation and limit species of macroinvertebrates that require a more

2-5 stable bottom. Community structure has changed little since pre-operational years, and the available evidence does not indicate that BVPS operations have affected the benthic community of the Ohio River (Table 2.7). 2.3 Fish

   ' 2.3.1 Objective Fish sampling was conducted to provide a continuous baseline of data and to detect possible changes that may have occurred in the fish populations in the Ohio River near BVPS.

2.3.2 Methods Adult fish surveys were scheduled and performed in May, July, September, and November 1998. During each survey, fish were sampled by standardized electrofishing techniques at four stations (Figure 1.3). Seining was performed at Station 1 (north shore) and Station 2B (south shore of Phillis Island), to sample species that are generally under-represented in electrofishing (e.g., young-of-the-year fish and small cyprinids). Night electrofishing was conducted using a boom electroshocker and flood lights mounted to the bow of the boat. A Coffett variable voltage, pulsed-DC electrofishing unit powered by a 3.5-kW generator was used. The voltage selected depended on water conductivity and was adjusted based on the amperage of the currant passing through the water. The north and south shoreline areas at each station were shocked for at least 10 minutes of unit "on" time (approximately five minutes each shore) during each survey. When large schools of fish of a single species were encountered during electrofishing efforts, all of the stunned fish were not netted and retrieved onboard the boat. A few fish were netted for identification, and the number of observed stunned fish remaining in the water was estimated. The size range of the fish in the school was also estimated and recorded. This was done in an effort to expedite sample processing and cover a larger area during the timed electrofishing run. Regardless of the number of individuals, all game fish were boated when observed. Fish selning was performed at Station 1 (control) and Station 2B (non-control) during each 1998 BVPS fishery survey. A 30 ft long bag seine made of 1/4-inch nylon mesh netting was used to collect fish located close to shore in 1 to 4 ft of water. Three seine

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   .                                                                                            I 2-6 hauls were performed at both Station 1 (north shore) and Station 2B (south shore of        l Phillis Island) during each survey.

Fish collected during electrofishing and seining efforts were processed according to l standardizedprocedures. Allcapturedgamefisheswereidentified, counted, measured for totallength (mm), and weighed (g). Non-game fishes were counted, and a random subsample of lengths was taken. Live fish were retumed to the river immediately after processing was completed. All fish that were unidentifiable or of questionable identification and were obviously not on the endangered or threatened species list were placed in plastic sample bottles, preserved, labeled and returned to the laboratory for identification. Any fish that had not previously been collected at BVPS was retained for the voucher collection. Any threatened or endangered species (if collected), would be

                                                                                                )

{ photographed and released. ' 2.3.3 Results 4 Fish population surveys have been conducted in the Ohio River near BVPS annually from 1970 through 1998. These surveys have resulted in the collection of 72 fish species and five different hybrids (Table 2.8). In 1998,328 fishes representing 33 taxa were collected (i.e., handled) during BVPS { surveys by electrofishing and seining (Tables 2.9 and 2.10). An estimated additional  ! 4,434 were observed but not handled during electrofishing surveys (Table 2.15). The most common species in the 1998 BVPS surveys, collected by electrofishing and l seining combined, were g!zzard shad (17 percent), redhorse species (14.3 percent), emerald shiner (12.8 percent), and freshwater drum (12.5 percent). The remaining species combined accounted for 43.4 percent of the total handled catch. The most frequently observed (handled and not handled combined) fish in 1998 was the emerald shiner (estimated n=3,342), followed by the gizzard shad (estimated n=1,083), redhorse species (n=60), and freshwater drum (estimated n=53) (Tables 2.9,2.10, and 2.15). Game fishes collected during 1998 included channel catfish, black crappie, white bass, , bluegill, largemouth bass, smallmouth bass, muskellunge, sauger, and spotted bass. Game fishes represented 21.0 percent of the total handled catch.- A total of 241 fish, representing 23 taxa, was collected by electrofishing in 1998 (Table 2.9). Gizzard shad accounted for the largest percentage (18.3 percent) of the electrofishing catch in 1998 followed by freshwater drum (16.2 percent), sauger (11.2 percent), and golden redhorse (10.5 percent). The most frequently-collected game species was sauger (11.2 percent of all fish collected) followed by smallmouth bass (7.1 percent).

v l 1 2-7

 ' A total of 87 fishes representing 14 taxa was collected by seining in 1998 (Table 2.10).

Fish taxa collected included emerald shiner (44.8 percent), mimic shiner (19.5 percent), gizzard shad (13.6 percent), and spottall shiner (4.6 percent).

  ' A total of 115 fish representing 18 species was captured during the May 1998 sample event (Table 2.11) Fish were collected at all sample stations. A total of 86 fish was -

collected during electrofishing and 29 during selning. Gizzard shad was the most common species during electrofishing efforts, and mimic shinerwas the most common species captured by seining. Gizzard shad was the most common observed fish species in May (Table 2.15). A total of 69 fish representing 18 species was captured during the July 1998 sample event (Table 2.12). Fish were collected at all sample stations in July. A total of 56 fish J was collected during electrofishing and 13 during seining. Smallmouth bass the most ' common _ species during electrofishing efforts, and emerald shiner was the most common species captured by seining. During the September sample event,78 fish were collected (Table 2.13). Fish were collected at all sample stations. Freshwater drum was the most common species captured by electrofishing. The most common observed fish species was emerald shiner (Table 2.15). Gizzard shad was the most common species captured by seining. During the November sample event,66 fish were captured (Table 2.14). No fish were captured by seining at sample Station S-1 in November. Freshwater drum, gizzard shad, and golden redhorse were the most abundant species captured by electrofishing. Emerald shiner was the most abundant species captured by seining. At the request of the Pennsylvania Fish and Boat Commission, electrofishing catch rates were calculated as fish per minute (i.e., power on time) of sampling for 1996 through 1998. Electrofishing catch rates are presented in Tables 2.16,2.17, and 2.18 for fish that were boated and handled during the 19" through 1998 surveys by season. In 1998, the annual catch rate was 1.51 fish per electrofishing minute. The greatest i electrofiching catch rate was in May (2.18 fish / electrofishing minute). The lowest catch l rate was thserved in November (1.10 fish / electrofishing minute). In 1997, the annual catch rate was 3.52 fish per electrofishing minute. The greatest electrofishing catch rate was in May (6.23 fish /electrofishing minute). The lowest catch rate was observed in November (2.00 fish /electrofishing minute), followed by the fall survey (2.62 fish /electrofishing minute) and the summer survey (3.11 fish /electrofishing minute). i

2-8 in 1996, the annual catch rate was 0.95 fish perelectrofishing minute. High, turbid water during the spring effort which reduces electrofishing efficiency probably contributed to this comparably low catch rate. The greatest electrofishing catch rate occurred during Novemberwhen 1.24 fish per electrofishing minute were caught. Seasonal catch rates tended to increase from May(0.38 fish /electrofishing minute) through November (1.24 fish /electrofishing minute). 2.3.4 Comparison of Control and Non-Control Stations The electrofishing data (Table 2.9) did not indicate major differences in species composition between the control station (1) and the non-control Stations 2A,2B, and 3. 5 A greater number of fish representing more species were captured at non-control stations than control stations. This was most likely due to the extra effort expended at non-control stations versus control stations (i.e., there are three non-control stations and only one control station). The seine data for 1998 (Table 2.10) indicated no major differences in species composition between control and non-control stations. The totalnumber of fish captured at non-control stations wan larger than at the control station. 2.3.5 Discussion The results of the 1998 fish surveys indicate that there is a normal community structure in the Ohio River in the vicinity of BVPS based on species composition and relative abundance of fish observed during the surveys. Forage species were collected in the highest numbers. Variations in annual catch are attributable to normal fluctuations in the population size of the forage species and the predator populations that rely on them. Forage species, such as gizzard shad and emerald shiner with high reproductive potentials, frequently respond to changes in natural environmental factors (competition, food availability, cover, and water quality) with large fluctuations in population size. ' This, in tum, influences their appearance in the sample populations during annual

     . surveys. Spawning / rearing success due to abiotic factors is usually the determining factor of the size and composition of a fish community.

Also, differences in electrofishing catch rate can be attributed to environmental conditions that prevail during sampling efforts. High water, increased turbidity, and swift currents that occur during electrofishing efforts in some years can decrease the collection efficiency of this gear.

                                                                                                       .w

2-9 in 1998, species composition remained comparable among stations. Common taxa collected in the 1998 surveys by all methods included gizzard shad, freshwater drum, emerald shiner, mimic shiner, redhorse species, channel catfish, buffalo species, common carp, sauger, quillback, and smallmouth bass. Little difference in the species composition of the catch was observed between the control (1) and non-control stations (2A,2B and 3). Habitat preference and availability are probably the most important factors affecting where and when different species of fish are collected. 2.4 Corbicula Monitoring Program 2.4.1 Introductiori The introduced Asiatic clam (Corbicula fluminea) was first detected in the United States . In 1938 in the Columbia River near Knappton, Washington (Burch 1944). It has since spread throughout most of the country, inhabiting any suitable freshwater habitat. Information from prior aquatic surveys has demonstrated the presence of Corbicula in the Ohio River in the vicinity of the BVPS, and the plant is listed in NUREG/CR-4233 (Counts 1985). One adult clam is capable of producing many thousands of young called earlyjuveniles. These early juveniles are very small (approximately 0.2 mm) and will easily pass through the water passages of a powerplant. Once thejuveniles settle on the substrate, rapid growth occurs. If Corbicula develop with!n a power plant's water passages, they can impede the flow of water through the plant, especially through blockage of condenser tubes and small service water piping. Reduction of flow may be so severe that a plant shutdown is necessary. Corbicula are of particular concem when they develop undetected in emergency systems where the flow of water is not constant (NPC, IE Bulletin 81-03). The Corbicula Monitoring Program at BVPS includes sampling the Ohio River, and the circulating river water and service water systems of the BVPS (intake structure and cooling towers). This report describes this Monitoring Program and the results of the field and plant surveys conducted through 1998. 2.4.2 Monitoring (a) Objectives The objective of the ongoing Monitoring Program is to evaluate the presence of Corbicula at BVPS and the Ohio River in the vicinity of the intake structure, to

2-10 evaluate the potential for and timing ofinfestation of the BVPS. This program is also used to monitor for the presence of zebra mussels (see Section 2.5). (b) Methods (1) Cooling Towers - Monthly Reservoir Sampling Corbicula enter the BVPS from the Ohio River by passing through the water intakes, and eventually settle in low flow areas including the lower reservoirs of Units 1 and 2 cooling towers. The density and growth of these Corbicula are monitored by collecting monthly samples from the lower reservoir sidewalls and sediments by using a sampler. The sampler used on the sidewalls consists of a D-frame not attached behind a 24-inch long metal scraping edge. This device is connected to a pole long enough to allow the sampler to extend down into the reservoir area from the outside wall of the cooling tower. Each month, a single petite ponar grab sample was taken in each reservoir to obtain density and growth information from the bottom sediment. The i samples collected from each tower were retumed to the laboratory and processed. Samples were individually washed, and any Corbicula removed and rinsed through a series of stacked U.S. Standard sieves that ranged in mesh size from 16.0 mm to 0.6 mm. Uve and dead clams on each sieve were counted and the numbers were recorded. The size distribution data obtained using the sieves reflects clam width, rather than length. Samples containing a small number of Corbicula were not sieved; individuals were measured and placed in their respective length categories. After discussions with DLC, it was decided to eliminate Corbicula monitoring during the winter months of December and January. The life he, tories of both macrofouling bivalves present at BVPS (i.e., Corbicula and zebra mussels) minimizes the need to monitor for them during these winter months. Settlement of spat (i.e., juvenile bivalves) does not successfully take place, and growth of any settled individuals is minimal. This schedule was first emplaced for December 1997. l I (2) Cooling Towers - Corbicula Density Determination Population surveys of both BVPS cooling tower reservoirs have been l conducted during scheduled outages (1986 through 1997) to estimate the number of Corbicula present in these structures. In 1998, neither Unit 1 or

I

                                                                                          \

o 2-11 i

         ' Unit 2 was sampled during a scheduled outage to estimate the Corbicula population.

(c)- Results (1) Unit 1 Cooling Tower - Monthly Reservoir Sampling in 1998, a total of 46 Corbicula (41 percent alive) were collected from the Unit 1 cooling tower basin during monthly reservoir sampling. The largest live Corbicula collected measured 16 mm in length (Table 2.19). The greatest number of Corbicula were collected in September (25 individuals) and October (17 individuals). Corbicula were collected in lower numbers in April, May, and November. 1 in 1998, DLC continued its Corbicula control program (ninth year) which l Included the use of a molluscicide (CT-1) to prevent the proliferation of Corbicula within BVPS. BVPS was granted permission by the Pennsylvania Department of Environmental Protection to use CT-1 to target the Unit i river water system and the Unit 2 service water system. In 1990 through 1993, the molluscicide applications (CT-1) focused on reducing the Corbicula population throughout the entire riverwater system of each BVPS plant (Units 1 and 2). In 1994 and 1995, the CT-1 applications-targeted the internal water systems, therefore the CT-1 concentrations in the cooling towers were reduced during CT-1 applications. Consequently, adult and juvenile Corbicula in the cooling towers often survived the CT-1 applications. Reservoir sediment samples taken after CT-1 applictHons represent mortality of Corbicula in the cooling tower only and do not reflect mortality in BVPS intemal water systems. CT-1 applications occurred on September 30, 1998 for Unit 1 and on October 13,1998 for Unit 2. (2) Unit 2 Cooling Tower - Monthly Reservoir Sampling in 1998, three Corbicula (33 percent alive) were collected from the Unit 2 ) cooling tower reservoir during monthly sampling. The largest Corticula collected was dead and measured 1.5 mm in length (Table 2.20). Two individuals were collected in April and one in November. A long-term unit outage may have contributed to these low numbers.

l 2-12 j (3) Cooling Towers -Corbicula Density Determination

                  . Unit 1 Cooling Tower No Corbicula density determination collections were made during 1998 for Unit 1 cooling tower.
                                                                                                )

Unit 2 Cooling Tower No .Qp_rtylgg!g density determination sampling was conducted in the Unit 2 cooling tower reservoir in 1998. (d) Discussion The monthly reservoir sediment samples collected in Units 1 and 2 cooling 1 towers during 1998 indicated that Corbicula were entering and colonizing the { reservoirs. The monthly clam density estimates for Unit 1 (Figure 2.1) indicated { a small population in May and then fell off to zero from June through August. l The largest numbers of Corbicula were observed in September and October. in November, the population decreased. No Corbicula were collected from Unit f l 2 until November (Figure 2.2). 2.4.3 Corbicula Larvae Study ' (a) Objective The Corbicula larvae study was designed to collect data on Corbicula spawning activities and growth of individuals entering the intake from the Ohio River. Methods I (b) Specially constructed clam cages were initially utilized for this study. Each cage 2 was constructed of a 1 ft durable plastic frame with fiberglass screening (1 mm mesh) secured to cover all open areas. Each cage contained approximately 10 lbs of industrial glass beads (3/8-inch diameter) to provide ballast and a uniform substrate for the clams. The clam cage mesh size permits only very small clams or pediveliger larvae to enter and colonize the cage. in 1988 through 1994, the cages were left in place for five months following initial placement. Changes in procedure were made to better define the time

1 2-13 period when Corbicula were spawning in the Ohio River and releasing larvae that could enter BVPS through the intake structure. Larval cages were maintained in the BVPS intake structure in 1995 according to the following procedure. Each month, two empty clam cages were placed in the intake structure bays. Each cage was left in place for two months, after which time it was removed and examined for clams. Four clam cages were maintained in the intake structure bays each month throughout 1995-1996. I l In February 1996, it was decided to modify the sampling regime so that two of  ! the four cages in the forebay were long-term samplers and the other two were monthly short-term samplers. Each month, the two long-term samplers were pulled; the fine sediment was carefullywashed from the cage and any Corticula present were measured. The cages were immediately redeployed along with ) any identified Corbicula. The two short-term cages were pulled monthly and the contents removed for laboratory analyses. New short-term cages were then deployed. Each short-term clam cage removed after the one or two month colonization period was retumed to the laboratory where it was processed to obtain the clams which had colonized inside the cage. Corbicula obtained from each cage were rinsed through a series of stacked U.S. Standard sieves ranging in mesh size from 9.5 mm to 0.6 mm. Live and dead clams on each sieve were counted and the numbers were recorded. The largest and smallest clams were measured using Vemier calipers to establish a length range for the sample. The size distribution data obtained using the sieves reflects clam width, rather than length. Observational-based concems that the clam cages could quickly clog with sediment during high sediment periods and, as a result, not sample effectively, led Acres to conduct an evaluation of an attemate sampling technique. From April through June 1997, Acres conducted a study to compare the results of the clam cage samplers to a petite ponar dredge technique to determine Corbicula presence and density in the BVPS intake bays, it was hypothesized that using a ponar sampler to collect bottom sediments and analysis of those sediments would provide a more representative sample of Corbicula settlement and growth rates, and had the added benefit of not requiring confined space entry to conduct the sampling. l 1 l l

y 2-14 During the 1998 sampling season, at the request of BVPS personnel, all clam cages were removed after the May 18, igg 8 collection. Monthly petite ponar grabs continued March through November. (c) Results Figure 2.3 illustrates size distribution data that represents the average for the petite ponar grabs for March through November. Corbicula first appeared in March and then again in July. The largest number of Corbicula in the ponar samples occurred in August and October. Discussion l (d) { 1 A late-spring /early-summer spawning period typically occurs in the Ohio River near BVPS each year when optimal spawning temperatures are reached (Figure 2.4). The offspring from this spawning event generally begin appearing

                                                                                                         )

in the sample collections in August and September (Figure 2.3). The offspring l will generally be observed to increase in size during subsequent sampling events under normal conditions. 2.5 Zebra Mussel Monitoring Program 2.6.1 Introduction Zebra mussels (Dreissena polymorpha) are exotic freshwater mollusks that have ventrally flattened shells generally marked with attemating zig-zag dark bands. They are believed to have been introduced into North America through the ballast waterof ocean-going cargo vessels probably from Eastem Europe. They were first identified in Lake St. Clair in 1987 and spread rapidly to other Great Lakes and the Mississippi River drainage system, becoming increasingly abundant in the lower, middle, and upper Ohio , Riverin recent years. 1

         - Adult zebra mussels can live up to five years and grow to 2 inches in length. North           l American research suggests that each female may be capable of producing over one million microscopic (veliger larvae) offspring per year, that can easily pass through water intake screens. They use strong adh a sive byssal threads, collectively referred to      '

as the byssus, to attach themselves to any hard surfaces (e.g., boat hulls, intake pipes and other mussels). Transport of these organisms between waterbodies is accomplished in part by boats that have adult mussels attached to their hulls or larvae in their live wells and/or bilges, in anticipation of zebra mussel infestation and

a 1 2-15 responding to NRC Notice No. 89 76 (Biofouling Agent-Zebra Mussel, November 21, 1989), BVPS instituted a Zebra Mussel Monitoring Program in January 1990. , The Zebra Mussel Monitoring Program included the Ohio River and the circulating river water system of the BVPS (intake structure and cooling towers). This section describes this Monitoring Program and the results obtained during Ohio River and BVPS surveys conducted through 1998. 2.5.2 Monitoring (a) _ Objectives The objectives of the Monitoring Program were: (1) To identify if zebra mussels are in the Ohio River adjacent to BVPS and provide early warning to operations personnel as to their possible infestation; (2) To provide data as to when the larvae are mobile in the Ohio River and insights as to their vulnerability to potential treatments; and (3) To provide data as to their overall density and growth rates under different water temperatures and provide estimates as to the time it requires for these mussels to reach clogging size and density. l l (b) Methods l (1) Intake Structure and Barge Slip i The surveillance techniques used in the intake structure and open water were: I Wall scraper sample collections on a monthly basis from the barge slip and the riprap near the intake structure to detect attached adults; Pump samples from the barge slip for detection of the planktonic early life forms (April through November); and . 1

  • Substrate plates were used for detection of settled stages in the impact basin below the Emergency outfall.

i

(,. 2-16 l (2) Cooling Towers I The techninues used in the Unit 1 and Unit 2 cooling towerlocations were:

          . Monthly reservoir scraper samples in each cooling tower; and
          . Pump samples in October and November to detect planktonic life forms.

(3) _ Emergency Outfall The emergency outfall was sampled from June through November to monitor for planktonic life forms of zebra mussels. (4) Impact Basin The impact basin was sampled from June through November to monitor for planktoniclife forms. (5) Results Zebra mussels were detected in both pump samples (Table 2.21) and substrate samples (Table 2.22). Zebra mussel veligers were present in pump samples collected from August through November. The greatest density of veligers was present in the splash pool sample collected in August (2,800/m'). Beginning in October, samples were not preserved, so live / dead determination could be made, in October, live veligers were present at all locations except in the cooling towers. In November, live veligers were also preset in the cooling towers. It is questionable whether the mussels in the cooling towers will survive the high summertime temperatures that exceed the mussels' thermal maximum. Juvenile mussels were observed in clam cage and ponar dredge samples from Intake Structures A and D at BVPS (Table 2.22). Juvenile zebra i mussels first appeared in the March samples and later appeared from September through November. No settled zebra mussels were observed on the substrate plates from the emergency outfall.

n , e 2-17 l (d) Discussion ' From 1991 through 1993, based on reports, zebra mussels moved progressively upstream from the lower to upper Ohio River. In 1994, there were confirmed i zebra mussel sightings atlocations both upstream and downstream from BVPS, including the Allegheny River. The July 1995 sighting of zebra mussels at Maxwell Locks and Dam on the Monongahela River established the presence of these organisms within the Allegheny, Monongahela and Ohio Rivers in Westem Pennsylvania. In 1995, live zebra mussels were found by divers in the BVPS main intake structure and auxiliary intake structure during scheduled cleaning operations. The 1996 Zebra Mussel Monitoring Program at BVPS did not collect any live zebra mussels at BVPS. During the first quarter 1996 (January and February) intake bay cleaning, divers observed an undetermined number of zebra mussels in the intake bays. During the second quarter 1996 cleaning, no mussels were reported. During the third and fourth quarter 1996 intake bay cleanings, about one dozen mussels were observed each time in Bay C only. None were collected by the divers for confirmation. During 1997, zebra mussel veligers were observed in June. Juvenile zebra mussels appeared in the clam cage and ponar dredge samples. In November 1997, adult zebra mussels were found in the intake ponar dredge samples. During the 1998 Zebra Mussel Monitoring Program at BVPS, zebra mussel veligers, juveniles, and an adult were observed in sample collections. A moderate density of zebra mussel veligers was observed during the August through November 1998 samples, indicating that spawning occurred sometime during the late summer. Juvenile zebra mussels appeared during March . I sampling. These mussels were 3.5,3.5, and 4.5 mm in length, which indicates that they were probably young-of-the-year in 1997. Young-of the-year zebra j mussels appeared in September through November. This observation confirms successful zebra mussel spawning in the area around BVPS. During 1998, zebra mussels were also found on the walls of the main intake structure during each of the quarterly inspections that took place. During the j first quarter, greater than 100 zebra mussels /ft2 were present in Bay B, although fewerwere presentin the other bays. Less than 5 mussels /ft2 were observed during the second quarter inspection that took place in April. Only Bays A and B were inspected, however. A few smaS .abra mussels were observed during i

                                                                                           )

2-18 the third quarter inspection; however, any recently-settled mussels would be easily missed during a visual inspection. Few (>10/ft)2 mussels were also observed during the fourth quarterinspection. Corbicula were also present in the main intake structure during each quarterly inspection. Zebra mussels were also observed in the altemate intake structure during the last three quarters of 1998, however, densities were low. '

w G c e D v 5 5 5 o N t 0 0 c 2 2 O 7 7 7 8 p 1 1 1 1 e & S 7 1 g 7 7 2 2 u A S 9 8 8 P l u 2 2 2 V & - 8 J 8 - B 9 2 Y91 N n 2 2 AR u 1 1 PO J 1 MF 2 OS y 8 1 8 1 1 9 8 1 E CE a & 8 T T M 8 9 H A L 1 9 B G D 1 g A n T L G I r 2 2 i r 2 2 N p d u EI L A - N P d e S t c EM r 5 2 e UA a l l M & o c QS 5 e U r e D b w e s F e lp m n a s a _ J y it s n e l e d e s r - t s e a r u w b M

  • t o _

e y r _ t ar it e g _ y r e b s ig n v e n le i l o d n e t u i o Z D V o c d l S r c n T e a a h a C s s s lu M i F a a u ic c lu lu M br ih ic i c a o r t n br br b C e o o e o B C C Z N

b TABLE 2.2 SYSTEMATIC LIST OF MACROINVERTEBRATES COLLECTED FROM 1973 THROUGH 1998 IN THE OHIO RIVER NEAR BVPS Collected in Collected in Nowin Taxa Ernyjous Years 1998 1998 Porifera Soonailla fraaths X Cnidaria Hydrozoa Clavidae Cordvloohora lacustris X Hydridae crasoedacusta sowerbli X liydtg sp. X Platyhelminthes Tricladida X Rhabdocoela X Nemertea X Nematoda X X Entoprocta Urnatella gracilis X Ectoprocta Fredericella sp. X Paludicella articulata X Pectinatella sp. X Plumatella sp. X { Annelida  ! Oligochaeta X X Aeotosomatidae X Tubificida X ) Enchytraeidae X X t Naididae X

  • Allonais pectinata X Amohichaeta leydtal X ,

Amohichaeta sp. X Arcteonais lomondi X Aulochorus sp. X l Chaetoaaster diaohanus X 1 Q. diastrochus X Qata digitata X QAIQ flabelliaer X Q.DIEta X l QRIEsp. X Nais barbata X N. behninal X

           - N. bretscherl                         X N. commuDit                            X TABLE 2.2 (Cont'd)

Collected in Collected in New in Taxa Previous Years 1998 1998 N. elinauts X N.p.atdaf!s X X N. oseudobtusa X N. simplex X N. variabihs X X Nais sp. X Oohidonais seroentina X Paranais fdd X Paranais sp. X Pouetiella michiaanensis X Pristina idrensis X Pristina tonaisoma X Pristina lonaiseta X E. s21ko.ml X E.nima X Pristina sp. X Pristinella osborni Ripistes parasrta X Slavina aooendiculata X Steohensoniang trivandrana X Stylaria fossularis X S. lacustris X Uncinais uncinata X Veldovskvella romata X Veldovskvella intermedia X Veldovskvella sp. X Tubificidae X Aulodritus limnobius X

6. pgueti X
6. oluriseta X Autodrilus sp. X Bothrioneurum veldovskvanum X Branchiura sowerbvi X livodritus templetoni X Limnodritus cennx X L. cervix (variant) X L. claoaredianus X L. hoffmeisterl X X L maumeensis X X

_ L orofundicla L. soiralis X l L. udekemianus X Limnodrilus sp. X Peloscolex multisetosus lonaidentua X E. m. multisetosus X Potamothrix moldaviensis X E. veidovskvi X X Psammorvctidgg curvisetosus X Tubifex tubifex X i Unidentified immature forms: l with hair chaetae X X without hair chaetae X X  ; Lumbriculidae X j J

r-TABLE 2.2 (C:nt'd) l Collected in Collected in Newin Taxa Previous Years 1998 1916. Hirudines X Glossiphoniidae X Helobdella elonaata X

11. stannahs X Helobdella sp. X Erpobdellidae Erpobdella sp. X Mooreobdella microstoma X Arthropoda Acarina X Ostracoda X lsopoda Asellus sp. X Amphipoda Talitridae Hvalella AZLtaa X Gammaridae Cranaonvr pseudogracihn X Cranaonyx sp. X-Gammarus fasciatus X Gammarus sp. X- X Decapoda X Collembola X I
                                                                                  -l Ephemeroptera                                             X                     l Heptagoniidae                       X                                        {

Stenacron sp. X I Stenonema sp. X Ephemeridae Eohemera sp. X Hexaoenia sp. X Eohron sp.- X-Baetidae X Baetis sp.- X Caenidae . Caenis sp. X

  • Serattella sp. X Potamanthidae -  !

Potamanthus sp. X l Tricorythidae l Tricorvthodes sp. X X Megaloptern SlaHg sp. X Odonata i Gomphidae . Ar.gla sp. X j Dromooomohus199]ialut X l Dromoaomohus sp. X TABLE 2.2 (C:nt'd) Collected in Collected in Newin Taxa Previous Years 1998 .1998 Gomohus sp. X Libellulidae -l i Libellula sp. X Trichoptera Hydropsychidae X Cheumatoosvche sp. X Hvdroosvche sp. X Psychomyiidae Psychomvia sp. X

  • Hydroptilidae Hydrootila sp. X Orthotrichia sp. X
  • Oxvethira sp. X Leptoceridae Ceraclea sp. X Oecetis sp. X X 1 Polycentropodidae Cvrnelius sp. X X Polvcentronus sp. X Coleoptera X Hydrophilidae X Elmidae X
  • Ancvronyx varieaatus X Dubirachia sp. X Helichus sp. X Stenelmis sp. X PsephenMae X Diptera Unidentified Diptera X Psychodidae X Pericoma sp. X Psychoda sp. X Telmatosconus sp. X i

Unidentified Psychodidae pupae X Chaoboridae Cnaoborus sp. X Simuliidae Similium sp. X Chironomidae X X Chironominae X X Tanytarsini pupa X Chironominae pupa X X Axarus sp. X Chironomus sp. X X Cladocelma sp. X Crvotochironomus sp. X X Dicrotendices nervosus X ' Dicrotendices sp. X X Givototendices sp. X X Harnischia sp. X 4

E TA'lLE 2.2 (C:nt'd) Collected in Collected in Newin Taxa Previous Years 1998 1998 Micrcchironomus sp. X Mcroosectra sp. X Merotendines sp. X Parachironomus sp. X X Paraciadopelma sp. X X Paratanvtarsus sp. X

  • Ear.atendipes alhimanus X Phaenoosectra sp. X X Polypedilum (s.s.) convictum type X E. (s.s.) simulans type . X Polvoedilum sp. X X Rting.tapvtarsus sp. X X Stenochironomus sp. X Stictochironomys sp. X Tanvtarsus coffmani X
  • Tanvtarsus sp. X X Tribelos sp. X Xenochironomus sp. X Tanypodinae X
  • Tanypodinae pupae - X Ablabesmvia sp. X X Clinotanvous sp. X
  • Coelotanvous scaoularis X Coelotanvous sp. X X Dialmababsta pulcher X Dialmabatista sp. X Prociadius (Prociadius) X Procladius sp. X X Tanvous sp. X Thienemannimvia group X X Zavrelimvia sp. X Orthociadiinae X Orthocladiinae pupae X Cricotoous hicinctus X Q. (s.s.) trifascia X Cricotoous (Isociadiusi-
             -svivestris Group                    X Q. (isociadius) sp.                         X Cricotoous (s.s.) sp.                       X                   X           ,

Eukiefferiella sp. X Hydrobaenus sp. X Limnochves sp. X Manocladius (s.s.) dratinctus X Nanocladius sp. X Orthocladius sp. X X Parametriocnemus sp. X Paraphaenocladius sp. X Psectrocladius sp. X X Pseudorthocladius sp. X Pseudosmittaa sp. X Smittia sp. X TABLE 2.2 (Cont'd) i Collected in Collected in Newin Taxa Previous Years 1998 1998 Diamesinae Diamesa sp. X Entthactia sp. X Ceratopogonidae X X Bezzia sp. X

  • Dolichopodidae X Empididae X X Wiedemannia sp. X Ephydridae X Muscidae X Rhagionidae X Tipulidae X Stratiomyidae X Syrphidae X Lepidoptera X Hydrachnidia X X Mollusca Gastropoda X X Physacea X  !

Physidae X Ehysa sp. X Ancylidae X Eenissia sp. X Planorbidae X X Valvatidae X X Valvata oerdeoressa X Pelecypoda X X Sphaerlacea X

  • Corbiculidae Corbicula fluminea X Corbicula sp. .. X X Sphaeriidae X Pisidium ventricosum X Pisidium sp. X Schaerium sp. X Unidentified immature Sphaerlidae X 1 Dreissenidae I Dretssgna golvmoroha X X i Unionidae X Anodonta QLandis X X Anodonta (immature) X Elhotso sp. X Unidentified irnmature Unionidae X 5-6-

TABLE 2.3 BENTHIC MACROINVERTEBRATE COUNTS FOR TRIPLICATE SAMPLES TAKEN AT EACH SAMPLE STATION BY SAMPLE DATE FOR 1998 Page 1 of 4 Collection Date: May 18,1998 Location Scientific Name 1 2A 281 2B2 2B3 3 Total Nematoda 2 5 14 21 Chironomidae 1 1 2 Coelotanvous sp. 1 1 Chironomus sp. 6 6 Phaenoosectra sp. 1 2 3 Cryptochironomus sp. 1 1 Polvoedilum sp. 29 40 8 3 80 Rheotanytarsus sp. 1 1 Tanvtarsus coffmani 1 1 Orthocladius sp. 14 2 1 17 CricotoDus (s s.) sp. 2 2 Ceratopogonidae 1 1

   .Qf1Zi a sp.                                                         1       1 Empididae                                                        1           1       -

l Gammarus sp. 1 2 1 1 5 Ephemeroptera 1 1 Potamanthus sp. 1 1 , l Tricorythodes sp. 2 2 Orthotrichla sp. 1 1 Oecetis sp. 1 1 Sphaeriacea 1 2 3 Corbicula sp. 1 1 12 18 2 34 Corbicula sp. half shell 1 1 Dreissena polymorcha 3 3

TABLE 2.3 BENTHIC MACROINVERTEBRATE COUNTS FOR TRIPLICATE SAMPLES TAKEN AT EACH SAMPLE STATION BY SAMPLE DATE FOR 1998 Page 2 of 4 Collection Date: May 18,1998 Location Scientific Name 1 2A 2B1 2B2 283 3 Total Hydrachnidia 2 2 Oligochaeta 2 2 Enchytraeidae 1 1 l Naldidae 1 1 f Nais cardalis 6 3 9 Nais variabilis 1 73 1 75 Tubificidae immature w/o hair 70 1 37 23 137 268 Tubificidae immature with hair 3 3 Limnodritus hoffmeistert 7 6 3 7 23  ! Limnodritus maumeensis  ! 1 1 l Potamothrix yeidovskyi 3 1 3 7 May 1998 Total: 118 98 90 27 68 181 582  ; l 1 l l l l l

TABLE 2.3 l BENTHIC MACROINVERTEBRATE COUNTS FOR TRIPLICATE SAMPLES l TAKEN AT EACH SAMPLE STATION BY SAMPLE DATE FOR 1998 Page 3 of 4 , l i Collection Date: September 17,1998 Location Scientific Name 1 2A 2B1 2B2 283 3 Total Nematoda 10 3 20 33 Chironomid pupae 3 1 4 Tanypodinae 1 1 Chironominae 1 1 Procladius sp. 6 21 1 58 86 l Thienemannimvia group 3 3 Coelotanvous sp. 3 11 14 Abfabesmvia sp. 3 1 4 Dicrotendices sp. 14 2 16 Givototendices sp. 3 1 4 Parachironomus sp. 11 1 3 1 1 36 53 Paratanvtarsus sp. 3 3 Crvotochironomus sp. 1 2 2 71 76 Polvoedilum sp. 10 3 21 1 35 70 Paraciadocelma sp. 1 1 Rheotanvtarsus sp. 2 2

  • Ignvtarsus sp. 8 2 4 14 Psectrocladius sp. 1 1 Clinotanvous sp. 1 1 Gammarug sp. 1 1 Baetis sp. 1 1 Oecetis sp. 3 3 Cvrnelius sp. 6 6

l

                                                                                        }

TABLE 2.3 i { BENTHIC MACROINVERTEBRATE COUNTS FOR TRIPLICATE SAMPLES TAKEN AT EACH SAMPLE STATION BY SAMPLE DATE FOR 1998 Page 4 of 4 Collection Date: September 17,1998 Location Scientific Name 1 2A 2B1 2B2 283 3 Total Psychomvia sp. 1 1 2 Elmidae 1 1 Gastropoda 4 1 5 Planorbidae 3 1 1 5 Valvatidae 4 1 5 Pelecypoda 30 2 3 14 49 Corbicula sp. 21 1 6 1 2 44 75 Corbicula sp. half shell 8 13 21 Anodonta grandis 8 8 Hydrachnidia 1 1 2 Maja variabilis 8 8 Tubificidae immature w/o hair 6 23 421 450 Limnodrilus hoffmeisteri 3 29 32 Limnodritus maumeensis 15 15 Potamothrix veldovskvl 20 4 8 7 39 September Totals: 176 27 105 4 7 796 1,115 GRAND TOTAL: 294 125 195 31 75 977 1,697 l 1 l

                                                                                        \

l l

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                          %                    1                 1 l                                                  .

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l TABLE 2.5 2 MEAN NUMBER OF MACROINVERTEBRATES (NUMBER /M ) AND PERCENT COMPOSITION OF OLlGOCHAETA, CHIRONOMIDAE, MOLLUSCA, AND OTHER ORGANISMS FOR THE CONTROL STATION (1) AND THE AVERAGE FOR NON-CONTROL STATIONS (2B1,2B2, AND 2B3),1998 BVPS I MAY 18 Control Station (Mean) Non-Control Station 2B (Mean)

                    #lm*                   %          #/m2                   %

Oligochaeta 1,210 71 374 42 , Chironomidae 446 26 288 32 Mollusca 14 1 168 19 Cthers 29 2 58 7 TOTAL 1,699 100 888 100 l SEPTEMBER 17 Control Station (Mean) Non-Control Station 2B (Mean)

                    #lm2                  %           #/m'                  %

Oligochaeta 374 15 163 29 q 1 Chironomidae 994 39 283 51 j Mollusca 965 38 67 12

                                             -                                           l l

Others 201 8 44 8 l TOTAL 2,534 100 557 100 l i I l l 1 l I

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TABLE 2.8 q SCIENTIFIC AND COMMON NAME' OF FISH COLLECTED IN THE NEW CUMBERLAND POOL OF THE OHIO RIVER,1970 THROUGH 1998 -{ BVPS  ! Page 1 of 3 Family and Scientific Name Common Name Lepisosteidae (gars) Leoisosteus osseus Longnose gar q I Hiodontidae (mooneyes) , Hiodon alosoides Goldeye I H. tergisus Mooneye Clupeidae (herrings) j Alosa chrysochloris Skipjack herring ) l

6. Dseudoherenaus Alewife Dorosoma ceoedianum Gizzard shad Cyprinidae (carps and minnows)

Camoostoma anomaluin Central stoneroller Carassius auratus Goldfish Ctenocharvnoodon idgjlg Grass carp Cvorinella soilootera Spotfin shiner Gyonnus caroto Common carp Q. carpio x Q. auratus Carp-goldfish hybrid Luxilus chrysocechalus Striped shiner Macrhvboosis storeriana Silver chub Nocomis micrococon River chub Notemiaonus crvsoleuca.s Golden shiner Notronis atherincides Emerald shiner N. buccatus Silverjaw minnow N. hudsonius Spottail shiner N. n!bellus Rosyface shiner H. stramineus Sand shiner H. volucellus Mimic shiner Pimechales notatus Bluntnose minnow E. oromelas Fathead minnow Rhinichthys atratulus Blacknose dace Semotilus atromaculatus Creek chub Catostomidae (suckers) Carniodes carpio River carpsucker Q. cvorinus Quillback G. Vetifer Highfin carpsucker Catostomus commersont White sucker Hvoentelium niaricans Northern hogsucker Ictiobus bubalus Smallmouth buffalo

1. DI Q21 Black buffalo Minvtrema melancos Spotted sucker C____________.__________..._.__.____.__._._.__.._ _ _ . _ _ _ _ _ _ _ _ _ _ ___ _ _ . _ _ _ _ _ . _ _ _ _ _ _ _ . _ . _ . _ _ _ _ _ _ _ _ _ _ _ _ _ . _ _ _ _ _ _ _ _ _ _ _ _ _

l .,. l l TABLE 2.8 (Continued) Page 2 of 3 Family and Scientific Name - Common Name l l Moxostoma anisurum Silver redhorse l M. carinatum - River redhorse ,

        . M. duauesnel                       Black redhorse                                 }
       - M, erythrurum                       Golden redhorse                                i M. macroteoidotum                   Shorthead redhorse letaluridae (bullhead catfishes)

Alneiurus catus White catfish A meias Black bullhead

6. patalis Yellow bullhead
6. nebulosus Brown bullhead letalurus ounctatus Channel catfish Noturus flavus Stonecat Pvlodictis olivaris Flathead catfish Esocidae (pikes) -
       . Esp 2 lucius                       Northern pike E. masauinongy                     Muskellunge E. luctus x E. masauinonav         Tiger muskellunge Salmonidae (trouts)

Oncorhynchus mykiss Rainbow trout Percopsidae (trout-perches) Perconsis omiscomavcus Trout-perch Cyprinodontidae (killifishes) Fundulug diaohanus Banded killifish

     ' Atherinidae (silversides)                                                            {

Labidesthes sicculus Brook silverside  ! l Percichthyldae (temperate basses) Morone chrvsoos White bass i

       . M. saxatilis                       Striped bass M. saxatilis x M. chrysoos         Striped bass hybrid Centrarchidae (sunfishes)

Ambloolites runestris Rock bass Lecomis cyanellus Green sunfish L. aibbosus Pumpkinseed L. macrochirus . Bluegill L. microlochus Redear sunfish L. oibbosus x L. nacroloohus Pumpkinseed-redear sunfish hybrid N!icrooterus dolomieu Smallmouth bass ,

       . E r%3qs .                           Spotted bass M. salmoides                       Largemouth bass Pomoxis annularis                  White crappie                                   j
       . E. niaromaculatus -                 Black crappie                                  l l

l

TABLE 2.8 (Continued) Page 3 of3 Family and Scientific Name Common Name Percidae (perches) Etheostoma biennioides Greenside darter E. nlarum Johnny darter  ; E. zonale Banded darter E21Qg flavescens Yellow perch Percina caorodes Logperch E. cocelandi Channel darter Stizostedion canadense Sauger S. vitreum Walleye S. canadense x S. M1reum Saugeye Sciaenidae (drums) Aolodinotus arunniens Freshwater drum

 ' Nomenclature follows Robins,3131. (1991)

1 , l TABLE 2.9 COMPARISON OF CONTROL VS. NON-CONTROL ELECTROFISHING CATCHES DURING THE BVPS 1998 FISHERIES SURVEY Non- Total l Common Name Scientific Name Control  % Control  % Fish  % l Mroneye ( Hiodon teraisus 3 1.6 3 1.2 I Gizzard shad Dorosoma caoedianum 10 18 34 18.5 44 18.3 Crmmon corp Cvorinus earoio 1 2 5 2.7 6 2.5 Golden shiner Notemloonus crvsoleucas 1 0.5 1 0.4 Emerald shiner Notroois atherinoides 3 1.6 3 1.2 Spottall shiner R hudsonius 3 1.6 3 1.2 Sand shiner R stramineus 1 0.5 1 0.4 Highfin carpsucker Catostomidae velifer 0.5 1 1 0.4 Quillback Caroiodes ervorinus 5 9 9 4.9 14 5.8 Smallmouth bunalo letiobus bubalus 3 1.6 3 1.2 B!ack bu#alo L nicer 3 5 5 2.7 8 3.3 Silver redhorse Moxostoma anisurum 5 9 16 8.7 21 8.8 Golden redhorse M erythrurum 3 5 22 12 25 10.5 Muskellunge Esox masouinonov 0.5 1 1 0.4 WhKe bass Morone chrysoos 5 9 9 4.9 14 5.8 Bluegill Lecomis macrochirus 1 0.5 1 0.4 Smallmouth bass Microoterus dolomieu 10 18 7 3.8 17 7.1 Spotted bass R ounctulatus 1 0.5 1 0.4 Lergemouth bass R salmoides 1 0.5 1 0.4 Black crapple Pomoxis niaromaculatus 1 0.5 1 0.4 Logperch Percina enorodes 1 1 5 2.7 6 2.5 Sruger Stizostedion canadense 4 7 23 12.5 27 11.2 Freshwater drum Aolodinotus crunniens 10 17 29 15.7 39 16.2 El;ctrofishing Gear Total: 57 100.0 184 100.0 241 100.0 l

TABLE 2.10 1 COMPARISON OF CONTROL VS. NON-CONTROL SEINE CATCHES DURING THE BVPS 1998 FISHERIES SURVEY - Non- Total Common Name Scientific Name Control  % Control  % Fish  % Gizzard shad Dorosoma ceoedianum 12 20.6 12 13.6 Common carp Cvorinus carolo 1 1.8 1 1.2 Emerald shiner Notroois atherinoides 17 58.5 22 37.7 39 44.8 SpottaR shiner Notroois atherinoides 4 13.8 4 4.6 Sand shiner R stramineus 1 3.4 2 34 3 3.4 Mimic shiner R volueellus 3 10.2 14 24.1 17 19.5 l Quillback Carniodes ervorinus 1 3.4 1 1.2 Golden redhorse Moxostoma erythrurum 1 3.4 1 1.2 Channel catHsh letalurus Dunctatus 1 1.8 1 1.2 Brook silverside Labideathes sleculus 1 1.8 1 1.2 White bass Morone chrysons 1 1.8 1 1.2 BluegiR Leoomis macrochirus 1 3.4 1 1.8 2 2.3 Smallmouth bass Microoterus dolomieu 1 3.4 1 1.8 2 2.3 Freshwater drum Aolodinotus arunniens 2 3.4 2 2.3  ; I Seine Gear Total: 29 100.0 58 100.0 87 100.0 l Sene and Year Tobl 86 - 242 - 328 - Electrofishing I l l l

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TABLE 2.16 ESTIMATED NUMBER OF FISH OBSERVED' j DURING ELECTROFlSHING OPERATIONS l l l Common Name Scientific Name May July September November Total Lengnose gar Leoisosteus osseus 1 1 j Gizzard shed Dorosoma cenedianum 280 36 594 117 1,027 Common carp Cynrinus carolo 3 6 6 16 31 8 Emerald shiner Notropis atherinoides 3,300 3,300 Mimic shiner Notrools volucellus 2 2 Buffalo sp. lctiobus an. 1 1 Black buffalo Ictiobus DigE 9 1 10 Redhorse sp. Moxostoma n. 3 3 Silver redhorse Morostoma anisurum - 2 2 Golden redhorse M. erythrurum 9 1 10 Catfish sp. letalurus E. 5 5 Channel catfish letalurus nunctatus 7 6 13 26 I Bluegill Lecomis ERGipchirus 1 i Logperch Percina caorodes 1 Freshwater drum Aolodinotus orunnlens 1 13 14 TOTAL 304 57 3,925 148 4,434 1

 '     Not bosted and handled.

i

r. 1 TABLE 2.16 CATCH PER UNIT OF EFFORT (CPUE AS FISH /ELECTROFlSHING MINUTE) BY SEASON DURING THE BVPS 1996 FISHERIES SURVEY Count of CPUE Season Effort (min) Common Name Species (fish / min) Spring 44.30 Spottail shiner 4 0.0903 Quillback 5 0.1129 Northem hogsucker 1 0.0226 Silver redhorse 1 0.0226 Channel catfish 1 0.0226 Striped bass 1 0.0226 White crappie 1 0.0226 Walleye 1 0.0226 Freshwater drum 2 0.0451 Season Total 17 0.3837 Summer 41.10 Longnose gar 1 0.0243 Gizzard shad 3 0.0730 Common carp 4 0.0973 Quillback 1 0.0243 Black buffalo 4 0.0973 Silver redhorse 15 0.3650 Golden redhorse 7 0.1703 Channel catfish 2 0.0487 Smallmouth bass 4 0.0973 l Log perch 1 0.0243 Sauger 2 0.0487 Freshwater drum 1 0.0243 Season Total 45 1.0949

TABLE 2.16 (Cent'd) CATCH PER UNIT OF EFFORT (CPUE AS FISH /ELECTROFlSHING MINUTE) BY SEASON DURING THE BVPS 1996 FISHERIES SURVEY Count of CPUE Season Effort (min) Common Name Species (fish / min) Fall 40.50 Gizzard shad 7 0.1728 Emerald shiner 21 0.5185 Silver redhorse 6 0.1481 Golden redhorse 1 0.0247 Channel catfish 1 0.0247 Striped bass 2 0.0494 Smallmouth bass 4 0.0988 Sauger 3 0.0741 Season Total 45 1.1111 Winter 45.10 Gizzard shad 8 0.1774 Alewife 1 0.0222 Spottall shiner 16 0.3548 Spotfin shiner 12 0.2661 Creek chub 1 0.0222 Qutilbaok 1 0.0222 Smallmouth buffalo 1 0.0222 Striped bass 1 0.0222 Smallmouth bass 3 0.0665 Black crappie 1 0.0222 Sauger 10 0.2217 - Walleye 1 0.0222 Season Total 56 1.2417 Year 171.00 183 0.9532

TACLE 2.17 CATCH PER UNIT OF EFFORT (CPUE AS FISHIELECTROFlSHING MINUTE) BY SEASON DURING THE BVPS 1997 FISHERIES SURVEY Page 1 of 3 Count of CPUE Season Effort (min) - Common Name Species (fishhnin) Spring 39.00 Gizzard shed 3 0.0769 Mooneye 2 0.0513 White sucker 1- 0.0256 Smallmouth buffalo 4 0.1026 Silver redhorse 27 0.6923 Golden redhorse 8 0.2051 Channel catfish 2 0.0513 Flathead catfish 1 0.0256 White bass 1 0.0256 Smallmouth bass 7 0.1795 White crappie 3 0.0769 Sauger 18 0.4615 Walleye 1 0.0256 Season Total ' 78 2.0000 Summer 45.40 Longnose gar 1 0.0220 Gizzard shad 49 1.0793 Silver chub 3 0.0661 Emerald shiner 40 0.8810 Spottall shiner 6 0.1322 Northern hogsucker 2 0.0441 Black buffalo 5 0.1101 Silver redhorse 17 0.3744 White bass 3 0.0661 Bluegill 1 0.0220 Smallmouth bass 3 0.0661 Logperch 1 0.0220 Sauger 4 0.0881 Walleye 3 0.0661 s

TABLE 2.17 (Cent'd) CATCH PER UNIT OF EFFORT (CPUE AS FISH /ELECTROFiSHING MINUTE)

                   ' BY SEASON DURING THE BVPS 1997 FISHERIES SURVEY Page 2 of 3 '

Count of CPUE Season Effort (min) Common Name Species (fish / min) Summer (cont'd) Freshwater drum 3 0.0661 Season Total 141 3.1087 Fall 40.10 Glzzard shed 34 0.8479 Skipjack herring 3 0.0748 Mooneye 3 0.0748 Goldfish 1 0.0249 Emerald shiner 2 0.0499 Spottail shiner 4 0.0998 Quillback 5 0.1247 Smallmouth buffalo 1 0.0249 Sihrer redhorse 11 0.2743 Golden redhorse 2 0.0499 Channel catfish 5 0.1247 White bass 7 0.1746 Striped bass 1 0.0249 Smallmouth bass 4 0.0998 Sau9er 2 0.0499 Freshwater drum 20 0.4988 Season Total 105 2.8184 , Winter 42.40 Gizzard shed 80 1.8868 Skipjack herring 1 0.0236 Mooneye 36 0.0708 Emerald shiner 80 1.8868 Spottail shiner 2 0.0472 Quillback 6 0.1415 White sucker 3 0.0708 Black buffalo 5 0.1179 Silver redhorse 19 0.4481

TABLE 2.17 (Cant'd) CATCH PER UNIT OF EFFORT (CPUE AS FISHIELECTROFiSHING MINUTE) 1 BY SEASON DURING THE BVPS 1997 FISHERIES SURVEY l Page 3 of 3 '{

                                                                                                                                                                                          '1 Count of    CPUE Season                                                                                                Effort (min)               Common Name              Species  (fish / min)

Winter (cont'd) Channel catfish 3 0.0708 White bass 13 0.3066 Bluegill 1 0.0236 Smallmouth bass 5 0.1179 Yellow perch 1 0.0236 Walleye 1 0.0236 Freshwater drum 41 0.9670 Season Total 264 6.2264 Year 166.90 588 3.5231 I i l

l TABLE 2.18 CATCH PER UNIT OF EFFORT (CPUE AS FISHIELECTROFISHING MIN BY SEASON DURING THE BVPS 1998 FISHERIES SURVEY Season Count of CPUE Effort (min) Common Name Species (fish / min) Spring 39.5 Mooneye 1 0.0253 Gizzard shad 15 0.3797 Emerald shiner 2 0.0506 Quillback 8 0.2025 Smallmouth buffalo 3 0.0759 Black buffalo 7 0.1772 Silver redhorse 4 0.1013 Golden redhorse 11 0.2785 Muskellunge 1 0.0253 White bass 9 02278 Smallmouth bass 3 0.0759 Black crapple 1 0.0253 Logperch 2 0.0506 Sauger 12 0.3038 Freshwater drum 7 0.1772 Season Total 86 2.1769 ) Summer 40.0 Mooneye l 1 0.0250 Gizzard shad 5 0.1250 Common carp 6 0.1500 Golden shiner 1 0.0250 . Highfin carpsucker 1 0.0250 Quillback 6 0.1500 Silver redhorse 7 0.1750 Golden redhorse 2 0.0500 White bass 2 0.0500 Bluegill 1 0.0250 Smallmouth bass 8 0.2000 Largemouth bass 1 0.0250

TABLE 2.18 (Cent'd) CATCH PER UNIT OF EFFORT (CPUE AS FISH /ELECTROFISHING MINUTE) BY SEASON DURING THE BVPS 1998 FISHERIES SURVEY

                                                                                                    'l Count of    CPUE Season     Effort (min)           Common Name Species  (fish / min)

Summer (cont'd) Logperch 3 ' O.0750 Sauger 6 0.1500 Freshwater drum 6 0.1500 i Season Total 56 1.4000 l Fall 40.0 Gizzard shad 14 0.3500 Spottall shiner 3 0.0750 Black buffalo 1 0.0250 j Silver redhorse 8 0.2000 I Golden redhorse 3 0.0750 1 Smallmouth bass 4 0.1000 Logperch 1 0.0250 l Sauger 6 0.1500 Freshwater drum 15 0.3750 Season Total 55 1.3750 i Winter 40.0 Mooneye 1 0.0250 Gizzard shad 10 0.2500 Emerald shiner 1 0.0250 Sand shiner 1 0.0250 Silver redhorse 2 0.0500 Golden redhorse 9 0.2250 White bass 3 0.0750 l Spotted bass 1 0.0250 Smallmouth bass 2 0.0500 Sauger 3 0.0750 Freshwater drum 11 0.2750 Season Total 44 1.1000 Year 159.5 241 1.5110

TABLE 2.19 UNIT 1 COOLING RESERVOIR MONTHLY SAMPLING CORBICULA DENSITY DATA FOR 1998 FROM BVPS Area Mean Maximum Minimum Estimated Sampled Live or Length - Length Length CoNection Date Number (sq ft) Dead Count (mm) (mm) (per sq m) (mm) 01/98' - Live - - - - - Dead - - - - - 03/05/982'8 - Live - - - - - t Dead - - - - - 03/25/982 - Live - - - - - Dead - - - - - 04/22/98 0.25 Live 0 - - - 0 Dead 2 1.4 1.5 1.3 87 05/19/98 0.25 Live 1 16.0 16.0 16.0 43 Dead 0 - - - 0 06/12/98 0.25 Live 0 - - - 0 Dead 0 - - - 0 07/28/98 0.25 Live 0 - - - 0 Dead 0 - - - 0 08/27/98 0.25 Live 0 - - - 0 Dead 0 - - - 0 09/17/98 0.25 Live 14 4.3 10.0 1.0 609 Dead 11 3.1 10.0 1.0 479 10/20/98 0.25 Live 3 3.4 7.5 1.0 131 Dead 14 6.0 10.0 1.2 609 I 11/05/98 0.25 Live l 1 1.2 1.2 1.2 43 I Dead 0 -

                                                                                            -                0
                                                                            -,-                                  I I

12/98' - Live - - - - - Des', - -

                                                                                                                 )

Unit Summary Lis- 19 4.8 16.0 1.0 102' 1

                                            ~

i .4 ad 27 4.7 10.0 1.0 116'

   '    No sampling scheduled for January and December, 2

No collection due to unit outage. Staff could not gain access to the plant during February. February samples taken on March 5,1998. Average of monthly estimates.

TABLE 2.20 l i UNIT 2 COOLING RESERVOIR MONTHLY SAMPLING CORBICULA DENSITY DATA FOR 1998 FROM BVPS Area Mean Maximum Minimum Estimated Sampled Live or Length Length Length Nurnber Collectic'.e Date (sq ft) Dead Count (mm) ' (mm) (mm) - (per sq m) 01/98' - Live - - - - - Dead - - - - - 03/05/982'8 - Live - - - - - Dead - - - - - 03/25/988 - Live - - - - - Dead - - - - - 1 04/22/98 0.25 Live 0 - - - 0 Dead 2 1.4 1.5 1.3 87 05/19/98 0.25 Live 0 - - - 0 Dead 0 - - - 0 06/12/98 0.25 Live 0 - - - 0 Dead 0 - - - 0 07/28/98 0.25 Live 0 - - - 0 i Dead 0 - - - 0 08/27/98 0.25 Uve 0 - - - 0 Dead 0 - - - 0 09/17/98 0.25 Live 0 - - - 0 Dead- 0 - - 0 10/20/98 0.25 Live 0 - - - 0 Dead 0 - - - 0 11/05/98 0.25 Live 1 1.2 1.2 1.2 43 Dead 0 - - - 0 12/97' - Uve - - - - - I Dead - - - - - i Unit Summary Live 1 1.2 1.2 1.2 3' Dead 2 1.4 1.5 1.3 54 l ' No sampling scheduled for January and December. 8 No collection due to unit outage. Staff could not gain access to the plant during February. February samples were taken on March 5,1998. Average of monthly estimates.

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t. l I i 3-1 I 3 REFERENCES f Burch, J. Q.,1944. Checidist of West American Mollusks. Minutes,- Conchology Club of Southem Califomia 38:18. Commonwealth of Pennsylvania,1994. Pennsylvania's Endangered Fishes, Reptiles and  : Amphibians. Published by the Pennsylvania Fish Commission. Counts, C. C. 111, 1985. Distribution of Corbicula fluminea at Nuclear Facilities. Division of Engineering, U.S. Nuclear Regulatory Commission. NUREGLCR. 4233. 79 pp. Dahlberg, M. D. and E. P. Odum,1970. Anreaal cycles of species occurrence, abundance and diversity in Georgia estuarine fish populations. Am. Midl. Nat. 83:382-392. DLC,1976. Annual Environmental Report, Non-radiological Volume #1. Duquesne Ught Company, Beaver Valley Power Station.132 pp. DLC,1977. Annual Environmental Report, Non-radiological Volume #1. Duquesne Ught Company, Beaver Valley Power Station.123 pp. DLC,1979. Annual Environmental Report, Non-radiological Volume #1. Duquesne Ught Company, Beaver Valley Power Station.149 pp. 1 DLC,1980. Annual Environmental Report, Non-radiological. Duquesne Light Company, Beaver Valley Power Station, Unit No.1.160 pp. DLC,1981. Annual Environmental Report, Non-radiological. Duquesne Light Company, Beaver Valley Power Station, Unit No.1.105 pp. + Appendices. l DLC,1982. Annual Environmental Report, Non-radiological. Duquesne Ught Company, { Beaver Valley Power Station, Unit No.1.126 pp. DLC,1983. Annual Environmental Repor1, Non-radiological. Duquesne Light Company, Beaver Valley Power Station, Unit No.1.124 pp. + Appendix. DLC,1984. Annual Environmental Report, Non-radiological. Duquesne Ught Company, Beaver Valley Power Station, Unit No.1.139 pp. DLC,1985. Annual Environmental Report, Non-radiological. Duquesne Ught Company, Beaver Valley Power Station, Unit No.1 & 2.106 pp.. l i l l 1

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