ML030130181
| ML030130181 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 11/13/2002 |
| From: | Osborne J Tennessee Valley Authority |
| To: | Haskin E Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML030130181 (108) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 November 13, 2002 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6t" Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Ms. Evelyn Haskin
Dear Ms. Haskin:
TENNESSEE VALLEY AUTHORITY - DISCHARGE MONITORING REPORT FOR SEQUOYAH NUCLEAR PLANT Please find enclosed the Discharge Monitoring Report (DMR) of October 2002 for Sequoyah. Please contact me at (423) 843-7630 if you have any questions or comments.
Incerely, D. Osborne Acting Environmental Supervisor Signatory Authority for Richard T. Purcell Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Pmted on rcW pww 2
PERMITTEE NAME/ADDRESS (include FacilityNameloca don YfDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved DISCHARGE MONITORING REPORT (DMRJ OMB No 2040-0004 Name._-TV_.A - SEQUOYAH NUCLEAR PLANT (SUBR 01)
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Read instructior Is before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE MEASUREMENT 26.6 (04) 0 31/31 MODELD CENTIGRADE 00010 Z 0 0 :..DrEQi.
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NAME/TITLE PRINCIPAL EXECU]iVE OFFICER ,ICeflify under penally of law thatthis document and all.................predunderm I
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TELEPHONE DATE u.._ uo
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........... ... .I . ....... .... U1l.,^ U L. Cl,. VL M,,II.V Richard T. Purcell personnel propedy gather and evaluate the Information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng virnmental Supervisor -423 843-7630-- -02 11 1 the Information, the Information submitted Is- to the best of my knowledge and belief, true,-- Site Vice President accurate, and complete I am aware that there are significant penaties for submitting false NATURE OF PRINCIPAL EXECUTIVE YEAR MO DAY OFFICER OR AUTHORIZED AGENT AREA NUMBER information, Including the possibirtty of fine and imprisonment for knowing violations "TYPEDOR PRINTED , CODE I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshere No closed mode operation. The following injections occured" 1. PCL-222 (max. calc. cone. was 0 054mg/L-himit 0 100mg/L) 2 CL-363 (max. calc. conc. was 0 009mg/L-hlmit 0 100mg/L) 3 PCL-222/PCL-401 (max. calc cone. was 0 026mg/L-himit 0.1 00mg/L)
EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 2
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
PERMITTEE NAME/ADDRESS (Include Facilhty NameaLocallon ifDifferent) (DMR) (SUBR 01)
DISCHARGE MONITORING REPORT OMB No 2040-0004 V911&-TVA - SEQUOYAH NUCLEAR PLANT TN0 450 101 G - FINAL PERMIT NUMBEP IIDISCHARGE NUMBE IDIFFUSER DISCHARGE
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AIrN: Jerry D. Osborne NOTE: Read Instructions before completing this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS AVERAGE TEMPERATURE - C, RATE OF SAMPLE * ** 0.1 (62) 0 31 / 31 CALCTD CHANGE MEASUREMENT UPSTRM DEG.C MEASUREMENT 8S2.;i234 1:RIT0 0 DEG C....
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SAMPLE MEASUREMENT Xe~t NAME/TITLE PRINCIPAL EXECU1]VE OFFICER il Certify under penalty of law thatthis document and all attachments were prepared under my - (% i~0ELEPHONE T DATE
_________________________ idrectien or supemsion in accordance with a system designed to assure that qualified * ' /'.*./
Richard T. Purcell personnel property gather and evaluate the information submitted Based on my inquIry of the /
________________________ erson or persons who manage the system, or those persons directly responsible for gathenng- EninmnaSpevo.42 83760 2 11 4 the.informationntheminformation submitteoissub totthe besttoftmy knowledge
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Site Vice President accurate, and complete I am aware that there are significant penalties for submitting false
- ATURE OFPRINCIPAL EXECUTIVE
________________________ nformation, including the possibi~ty oi fine and Imripsonment for knowing violations OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED OR PRINTED __________________________CODE~
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference oil attachments here CCW data for October 2002 is attached.
EPA Form 3320-1 (REV 3199) Previous editions may be used P'age 2 of 2
CCW Data for October 2002 CCW CHANNEL DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mgq/L)
October 11, 2002 < 0.5 CCW TRENCH DISCHARGE DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mg/L)
October 11, 2002 0.8 Page 1
PERMITTEE NAME/ADDRESS (Include FacilityNameaocatfon I'Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved npameý- TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
OMB No 2040-00 04 (SUBR 01)
TN°°26450 [ 101T F - FINAL
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PERMITNUMBER DISCHARGE NUMER BIOMONITORING FOR OUTFALL 101 f~gJLty_ _A =QLEAR*- O EtR_P ,NT Loi(ZotIij-IAMILTON COUNTY MONIORINý'* PF 10 EFFLUENT I AY I , mn ATrN. Jerry D. Osborne From 0To*1o WFARlU MO o01 1To [-oE2a1"10l31 F* MO AY NO DISCHARGE ED NOTE: Read Instructlions before completing this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE * **>100 (23)*0 1/90 COMPOS CERIODAPHNIA MEASUREMENT TiPiT.P 149 0.T ::;i:
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Richard T. Purcell personnel property gather and evaluate the information submited Based on my inquIry of the
__________________________________person or persons who manage the system, or those persons directly responsible for gathering EvrnetlSpr~o 2 4 60 0 1 1 the Informat~on= the Information Submitted Is' to the best of my knowledge and belief, true.-* -- 4 3 - 4 -6 0- 2 -1 -- 1 Site Vice President accurate, and complete I am aware that there are slgnificant penalties for submitting false AT*UREOF PRINCIPAL EXECUTIVE TYPED OR PRINTEDOFIEORATOIEAEN AEA NME YAR O DY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference oit attachments here Toxicity was sampled October 6-11i. 2002. Report is attached.
EPA Form 3320-1 (REV 3/99) Previouseditions may be used Page 1 of 1
November 7, 2002 Richard D. Nunley, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, OCTOBER, 2002 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected October 6-11, showed no I toxic effects to fathead minnows or daphnids. The resulting IC 25 values for both species were ->100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples to remove pathogenic interferences. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to routine compliance samples and UV treated samples.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
CL'-JKý '->- LKý'Qx Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB 1B-M SQN October 2002m K
'-- )
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: November 7, 2002
- 1. Facility / Discharger: Seguoyah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN 0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD):- 3,266
- 7. 1Q10: 2,992.4
- 8. Outfall Tested: 101
- 9. Dates Sampled: October 6-11, 2002 @ \
- 10. Average Flow on Days Sampled (MGD): 1654Z 165V, 165)
Pertinent Site Conditions: No unusual conditions reported. (Production/
11.
operation data will be provided upon request).
- 12. Test Dates: October 8-15, 2002
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephalespromelas)
Daphnids (Ceriodaphniadubia)
- 15. Coincentrations Tested (%): Outfall 101: 10.98, 22, 43.9, 72, 100 Intake: 100 Pimephalespromelas: UV treated Outfall 101: 10.98, 22, 43.9, 72, 100 UV treated Intake: 100_0
- 16. Permit Limit Endpoint (%):
m
- f Outfall 101: IC.) = 43.9%
- 17. Test Resutits: Outfall 101: Pimephalespromelas:IC25 > 100 Ceriodaphniadubia: Ic 5 _> 100 UV treated Outfall 101: Pimephalespromelas:IC2 > 100
- 18. Facility Cc)ntact: Richard D. Nunley Phone #: (423) 843-7748
- 19. Consulting / Testing Lab: Environmental Testing Solutions, LLC
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 862-8193
- 21. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 22) Notes: Outfall 101 samples collected October 6-11, 2002, showed no toxic effects to fathead minnows or daphnids-. The resulting IC2 5 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples to remove pathogenic interferences. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to routine compliance samples and UV treated samples.
2
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101. Intake
- 2. Sample Type: Composite
- 3. Sample Information:
I Date Date Date (MM/DD/YY)/ (MM/DD/YY)/ Arrival Initial Sample Time (EST) Time (EST) Temp. TRC* Time (EST)
ID foo*
Colete
....... -Used 101 10/06/02 0740 to 10/08/02 1059 0.3t <0.10 10/08/02 1559 Intake- 10/07/020756 10/06/02 0641to 10/09/02 10/08/02 1059 0.3 <0.10 1555 10/08/02 1559 101 10/07/02 0656 10//02 10/08/02 1049 to 10/10/02 1052 0.8t <0. 10 1555 10/10/0211552 10/09/02 0949 10/11/0211520 Intake 10/08/02 1109 to 10/10/02 1052 0.8 <0.O1-0 10/10/0211522 .
10/09/02 1009 10/11/0211520 101 10/10/02 1329 to 10/12/02 1105 2.41 <0.10 10/12/02 1531 10/11/02 1230 10/13/02 1526 Intake 10/10/02 1342 to 10/12/02 1105 2.4 <0. 10 10/14/02 10/12/02 1524 11531 10/11/02 1242 10/13/02 11526
- TD I-t- = ^*r I1-,,, . . _
- 10/14/02 11524 tCollected o~**-
intat two Rkesidual U.,monne 2.5-gallon cubitainers. Temperature was measured in each cubitainer, upon arrival
- 4. Sample Manipulation: Samples from Outfall 101 and the Intake were warmed to test temperature (25.0 +/-l.00 C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples wereUV-treated through a 40-watt Rainbow Lifeguard UV Sterilizer for 2 minutes.
3
Test Organisms: Pimephalespromelas Ceriodaphniadubia
- 1. Source: Aquatic BioSvstems, Inc. In-house Cultures
- 2. Age: 24.5-27.5 hours5.787037e-5 days <br />0.00139 hours <br />8.267196e-6 weeks <br />1.9025e-6 months <br /> old < 24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of controol females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10 1O
- 5. Organisms per Replicate: 10
- 6) Test Initiation: (Date/Time)
Outfall 101 10/08/02-1559 EST 10/08/02-1219 EST UV Treated Outfall 101 10/08/02-1559 EST
- 7) Test Termination: (Date/Time)
Outfall 101 10/15/02-1544 EST 10/14/02-1159 EST UV Treated Outfall 101 10/15/02-1609 EST
- 8) Test Temperature: Outfall 101: Mean = 24.80 C Mean = 24.90 C (24.2-25.30 C) (24.3-25.3 0 C)
Test Temperature: U-V-Treated Outfall 101: Mean = 24.6°C (24.4-25.4 0 C)
- 9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures w( re measured in one replicate for each test concentration. Pre- and post exposure test solutions were analyzed daily for pH and dissolved oxygen
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
4
TOXICITY TEST RESULTS (see Appendix B for Bench Sheets)
- 1. Results of a Pimephalespromelas Chronic 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted October 8-15. 2002 using effluent from Outfall 101.
Test Percent Surviving Solutions time interval used - daa s)
(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 98 98 98 98 10.98% 100 100 100 100 100 100 100 100 100 100 100 100 100 100 22% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72% 100 100 100 100 100 100 100 100%
Intake 100 100 100 100 100 100 100 96-hour LC5 0 Value: > 100% Calculated TU Estimates: < 1.0 TUa*
95% Confidence Limits: NA Upper Limit: NA Permit Limit: 1.0 TUa Lower Limit: NA Mean Dry Weight (mg)
Test Solutions (re licate number)
(% Effluent) 1 2 3 4 Mean Control 0.982 0.988 0.862 0.929 0.940 10.98% 0.927 1.019 0.845 0.969 0.940 22% 0.958 0.904 0.900 0.920 0.920 43.9% 1.008 1.000 1.037 0.936 0.995 72% 0.903 1.017 0.827 0.930 0.919 100% 1.024 0.979 1.004 0.975 0.995 Intake 1.053 1.090 1.041 0.928 1.028 IC5 0 Value: > 100% Calculated TU Estimates: <1.0 TUc*
95% Confidence Limits: NA Permit Limit: 1.0 TUc Upper Limit: NA
- TUa = Lower Limit:
100/LCso: TUcNA= 100/ IC 25 5
TOXICITY TEST RESULTS (see Appendix B for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted October 8-14, 2002 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions 1 2 3 4 5 6 7
(% Effluent)
Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72%- 100 100 100 100 100 100 100 100% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 96-hour LC50 Value: > 100% Calculated TU Estimates: < 1.0 TUa*
95% Confidence Limits: NA Upper Limit: NA Permit Limit: 1.0 TUa Lower Limit: NA Reproduction (#young/female/7 days)
Test Solutions Data (replicate number)
(% Effluent) 1 2 3 4, 5 6 7 8 9 10 Mean Control 32 25 30 13 30 29 30 29 28 28 27.4 10.98% 31 32 31 25 30 15 29 30 31 32 28.6 22% 36 31 30 29 27 37 33 33 29 31 31.6 43.9% 32 29 30 34 30 39 34 35 30 30 32.3 72% 33 32 36 38 31 37 34 33 30 37 34.1 100% 34 36 36 33 39 35 20 37 37 41 34.8 Intake 32 30 30 32 27 20 33 14 28 28 27.4 IC 50 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits: NA Upper Limit: NA Permit Limit: 1.0 TUc Lower Limit: NA
- TUa = 100/LC5 0 : TUc = 100/IC25 6
TOXICITY TEST RESULTS, UV-TREATED (see Appendix B for Bench Sheets)
- 3. Results of a Pimephalespromelas Chronic 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted October 8-15, 2002 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2 3 4 5 6
, -7 Control 100 100 100 1 4 S100 15100 I 6 100 7
100 10.98% 100 98 98 98 98 98 98 22% 100 100 100 98 98 98 98 43.9% 100 100 100 100 100 100 100 72%- 100 100 100 100 100 100 100 100% 100 100 100 100 100 100 100 Intake 100 100 100 100 1A/'*
100 fi 100(1 100 1A 96-hour LC5 0 Value: > 100% Calculated TU Estimates: < 1.0 TUa*
95% Confidence Limits: NA Upper Limit: NA Permit Limit: 1.0 TUa Lower Limit: NA Mean Dry Weight (mg)
Test Solutions (replicate number)
(% Effluent) 1 2 3 J 4 91 Mean I I I --
Control 1.200 1.195 1.154 0.990 I I*ý 0.990 11 10.98% 0.982 1.000 0.995 0.959 0.984 22% 1.149 1.156 1.035 0.843 1.045 43.9% 1.191 1.090 1.025 1.102 1.102 72% 1.173 1.034 0.940 1.193 1.085 100% 1.150 1.081 1.270 1.122 1.156 Intake 1.185 1.117 1.052 1.30 1.096 1.030 1.096 IC 50 Value: > 100% Calculated TU Estimates: <1.0 TUc*
95% Confidence Limits: NA Permit Limit: 1.0 TUc Upper Limit: NA Lower Limit: NA
- TUa = 100/LC 50 : TUc = 100/ IC 25 -J 7
REFERENCE TOXICANT TEST RESULTS (see Appendix A and C)
Species Date Time Duration Toxicant Results (IC 25 )
Ceriodaphniadubia October 8-15, 2002 1116 7-days NaC1 1026.9 mW,/L Pimephalespromelas October 8-15, 2002 1500 7-days KC1 525.7 m
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected October 6-11, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples to remove pathogenic interferences. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to routine compliance samples and UV treated samples.
8
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephalespromelas Tests were conducted according to EPA/600/4-91/002 (July 1994) using four replicatesý each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia Tests were conducted according to EPA/600/4-91/002 (July 1994) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropjllene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas None
- 2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TES']
ORGANISMS
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None 9
PHYSICAL AND) (CHEMICAl. MV"TT-TOThV
- 1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured using EPA Method 170.1.
- 5. The pH was measured EPA Method 150.1.
- 6. Conductance was measured EPA Method 120.1.
- 7. Alkalinity Was measured using EPA Method 310.1.
- 8. Hardness was measured EPA Method 130.2.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPAI600/4-91/002. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix C for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC values in g KCI or NaCl.
25
Sodium Chloride (NaCI crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc softvare Version 5.0 was used for statistical analyses.
10
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. Lewis, P. A., D. J. Klemm, J. M. Lazorchak, T. J.
Norberg-King, W. H. Peltier, M. A. Heber.
Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPAI600/4-91/002 (July 1994).
- 3. Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
11
Sequoyah Nuclear Plant Biomonitoring October 08-15, 2002 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, And Initial and Final Chemistry for Fathead Minnow 7-day and Daphnid 3-brood Chronic Tests 0
Table B-1'. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations Chemicals Used to Control Growth of Microbiologically of Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-October/,2002 D~ate 2oddf~im Towerbrom :,*Lj2,- PCL-401 CEi363 Cw**- '* igL 777hl i Cuprostat- F- 4JOR
' PF mg/LTRC img/L mg/L
-Plios"hate Copolymer Azole .O0 t.
0.120
Table B-i (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101)
Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sam ling, March 12, 1998-October ,21002
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) of Chemicals Used to Control Growth of Microbiologically Discharge Concen Induced Bacteria and Asiatic During Toxicity Test Sampling, March 12,1998-Octoberri, 2002 Date 2,0 Towerbrom trCt221: PCL-401 ~G~6311 flnn TT1 Ii~i jmPF Smg/L Azole opolymer 0.02 0.014 0.014
-c oy.?
PHYSICAUCIIEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephalesproinelas and CeriodaphniadubiaTests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101, October 8-15, 2002.
Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pH(S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (limhos/em) (mg/L CaCO,) (mg/L CoCO3 ) Chlorine (mg/L)
Pimephales Control 24.5 25.0 80 7.2 7 87 7.51 306 61 83 pronmelas 24.2 - 247 248 - 253 78 - 84 69 - 7.7 7.71 - 797 725 - 779 294 - 314 60 62 82
- - 86 10.98% 246 250 80 7.2 7.87 745 1 293 244 - 24.7 248 - 253 78 - 82 69 - 7.7 782 - 7.94 7.20 - 778 283 - 302 22% 246 250 80 7.0 7.86 7.45 280 244 - 248 248 - 253 78 - 82 66 - 77 7.82 - 793 724 - 7.74 270 - 288 24.7 250 8.1 70 7.83 744 255 245 - 248 248 - 25.3 79 - 83 65 - 7.7 780 - 790 717 - 7.76 247 - 263 72% 24 7 25.0 8I 7.0 7.76 7.43 220 245 - 249 248 - 25.3 7.9 - 83 65 - 7.7 762 - 785 721 - 7.73 214 - 226 100% 248 250 8.1 70 7.74 742 188 62 82 <0 10 24.5 - 250 248 - 253 79 - 8.3 64 - 78 766 - 7.90 7.19 - 7.74 182 - 192 61 63 72 - 100 <0.10 - <010 Intake 24.7 250 8.2 7.2 7.67 741 186 60 83 < 0.10 24.4 - 252 248 - 253 80 - 84 64 - 80 742 - 7.84 721 - 781 181 - 195 57 - 62 80 - 86 <0.10 - <010 Ceriodaphnia Control 24.5 25.1 80 81 7.86 7.95 305 62 84 dubia 243 - 246 249 - 253 78 - 84 7.7 - 84 7.71 - 7.97 782 - 805 294 - 314 61 - 62 82 - 86 10.98% 24.6 25.1 80 81 7.88 7.96 292 244 - 247 249 - 25.3 78 - 82 7.7 - 8.4 783 - 7.94 7.75 - 810 283 - 301 22% 246 25.1 80 8.1 786 7.96 279 244 - 24.7 249 - 253 78 - 82 7.8 - 84 782 - 7.93 7.76 - 811 270 - 286 24 6 25.1 8.1 82 7.83 7.96 254 1 245 - 247 24.9 - 25.3 79 - 83 7.9 - 84 780 - 7.90 7.74 - 815 247 - 261 72% 24.7 25.1 81 8.2 7.76 7,96 220 246 - 249 24.9 - 253 79 - 83 7.9 - 84 762 - 7.85 7.71 - 813 214 - 224 100% 24.8 25.1 8.1 8.3 7.73 7.94 187 62 82 < 0.10 246 - 253 249 - 253 7.9 - 83 80 - 85 7.66 - 7.90 776 - 8.10 182 - 192 61 - 63 72 - 100 <0.10 - <010 Intake 248 25.1 82 8.2 766 7.90 186 60 83 <0 10 Intake 246 - 252 24.9 - 253 80 - 84 80 - 84 742 - 784 7.75 - 807 181 - 195 57 62 80 - 86
- <0.10 - <010
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the Pimephalespromelas Test, Sequoyah Nuclear Plant Effluent (SQN), U.V Treated Outfall 101, October 8-15, 2002.
Test Sample ID Temperature (0 C) Dissolved Oxygen (mg/L) PlI (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pmhos/cm) (mg/L CaCO3) (mg/L CRCO3 ) Chlorine (mg/L)
Pinsephales Control 245 250 80 7 1 786 7.42 299 61 83 promelas 244 - 247 24.7 - 25.2 7.7 - 85 64 - 7.8 7.77 ý 792 726 - 7.66 291 - 313 60 - 62 82 - 86 10.98% 24.6 250 80 7.1 784 7.42 293 245 - 249 24.7 - 252 77 - 85 64 -78 7.73 - 790 7.22 - 768 284 - 306 2% 24.7 25 0 8.0 7.1 7 82 7.40 280 22% 245 - 249 247 - 25.2 77 - 85 66 - 79 7.72 - 7.89 722 - 765 270 - 297 24.7 25 0 80 70 7.81 7.38 257 245 - 248 247 - 25.2 77 - 85 66 - 78 767 - 788 723 - 7.63 246 - 277 72% 24.7 25.0 80 7.0 7.79 7.38 222 245 - 24.9 247 - 252 76 - 85 66 - 7.7 765 - 7.86 724 - 7.62 214 - 237 100% 24.8 250 80 7.0 7.72 7.36 189 62 82 < 0.10 245 - 251 24.7 - 252 76 - 84 65 - 7.7 7.59 - 782 7.20 - 763 180 - 200 61 - 63 72 - 100 <010 -<0.10 Intake 24 8 25 0 8.0 7.0 7.70 7.39 185 60 83 < 0.10 245 - 254 247 - 252 7.7 - 84 66 -79 755 - 7.78 720 - 7.64 180 - 191 57 - 62 80 - 86 <0.10 - <010,
Sequoyah Nuclear Plant Biomonitoring October 08-15, 2002 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
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BIOMONITORING CHAIN OF CUSTODY RECORD Client: TVA Environmental Testing Solutions, LLC Project Name: TVA SQN Research Building UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Facility Sampled: SEQUOYAH NUCLEAR PLANT I Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 i
Collected By: RONALD LOWERY Field Identification /
Sample Description Instructions- Clients should fill in all areas except those in the "Laboratory Use" block. liomonitoring samples are preserved by storing them at 4C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefbre, please collect and ship in such a way that the laboratory-will receive the samples with ample time Samples shipped overnight on Friday via FedEx-or to initiate testing within that time-f-amfi?
UPS-ffiist be-ma-kdfor-Saturday delivery or they will not arrive until the following Monday.-.
.BIOMONITORING CHAIN OF CUSTODY RECORD Client: TVA rage I of I Project Name' TVA SQN Environmental Testing Solutions, LLC I De1 7"r i ",,,
Research Building P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Facility Sampled: SEQUOyAH NUCLEAR PLANT Phone: 828-862-8193 NPDES Number: 0026450 General Comments:
Fax: 828-862-8195 Collected By: RONALD LOWERY Field Identilication /
Sample Description n.1tructions.
- ample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> Clientsfrom should the fill timeinofallcollection areas except those in please the "Laboratory
,urbiples shipped overnight on Friday Therefore, collect andUse" shipblock.
in suchBiomonitoring a way that thesamples are preserved by storing them at 4*C and shipping them in ice. The via FedEx-or JPSiiiiite marked laboratory Will t-he- same hold-timefr each for Saturday delivery or they will not g within that time frame.
arrive until the following Monday.
I .
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):_
Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: RONALD LOWERY Field Identification/I Grab/ Ship. Collection Date/Time Number & Flow Rain Event?
Sample Description 5, AN Comp Temp CVolume (MGD) (Mark as Appropriate) 0 ........
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SQN-INT-TOX comp 4 10/10/02 10110 (2.5g)
-'i6~ I b Sample Custody - Fill In From Top Down-.,
Relinquished By (Signature): Date/Tinie Received By (Signature): Date/Time I°C)~UULLIUI1~
excptChos Io-16-o.
in snouiuiiiioraory inthe"La i~ziczus lO5 -
areasbock. d.Bio.onitoring.samples:are aiiUse
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,-, ,1CL o u36 hours is3 r from C.nsents ho fom the hettimei.eof snouiatin nofallcollection.n.TTherefore,please areasi except ole those rfrepin theeae collect olet andndUse" "Laboratory ship hpinblock. uhBiomonitoring awa that tht the hesamples ab aoy are preserved wll receive eci by ethe hestoring a pes themwith at ih 4"Capand etmetshipping in such awvay laboratory'will samples ample time to iitthem tettng initiate in ice. Ile testing ih holdnt att time for within that time frame.
efr eachsm e_
-Samples shi pped overnight on-Fridayrviaf FedEk-o-rJP muist be-markCed for Saturday delivery or they will not arrive until the following Monday.
b.
Chronic Whole Effluent Toxicity Test (EPA/600I4-91/002 Method 1000.0)
Species: Pimephalespromelas Client: "NI k Facility: ,CaQtOMAA MguNfA L(,J0 NPDES #: TN - m)C aL,45 No NTUAreib Dilution preparationinformation: Comments:
MHS batch: 1 10.!z Didutoprjep%) 1o.q9 2Z q3,q -z _00 Effluet volume (mL) j 'q.-7 .30 km. 5 1060 1 -0 _
Ddulunvolume (mL) 113531 Ip 12DI.I Test organisminformation: .. - 2,7.S Test information:
Organism age: I-qho or2 A p id Randomizing template: 15UA..
Date and times organisrmff IO*-31-O-2 ;0-10 -M i3o )AISC Incubator number:
were bor between: -030 ýo IS30 )D _ .3 Organism source: A6.ý &tA\ IO-0"1-O2, Artemia lot number. ik'&V l6 Transfer bowl information: pH = Temperature Oven temperature: 0 7C-,
-7 2.. I C Dryingtime: -/4 V4:
Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 2__ bO,552o2 101I3 2 lo-l.z O5£3 I6oo _
____ __--0 6 ,'-IVOZ, j00{ ItoS\ lIZc 2 6 01-s*O"1- iO0" Controlinformation: Aelta ~en' Summary of test endpoints:
% Mortality: 2 .5 . S20% 7-day LC5O Average weight per larvae: _a 9T/03 >_ 0.25 mg/lavae NOEC /0 o.
LOEC ChV 710,0'17, IC25 __1007.7_
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Survival and Growth Data Day ,7 -. I77tlX109 M N 0 P R S T U V W X 0 10 /o ) I10/0 10 )c /0 ) /0 I /1
/- 1 10 I0 10 I10 10( /0 0 10 I() /0 6 2 10 JO /0 1io 0 1I 10 It /0 IC) Io Ia 3 bI0 1 0 /0 /o /0 /0 /0 1 /0 /0 /0
/0 )0 I //0 I0 /0 /0 /0 /0 /0 /0
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zq Larvae weight (mg) -A-B j . /0. 312 1 15 1-1 ,j0.11 2 11 1.6. 55 q.1 '1 10.6 1 1H Calculations and data reviewed:
Comments:
pnvu-nmeunea "esnng solutions, LLC Page 4 ot 8 Species: Pimephalespromelas Client: -1A- 35e.
' ýao Date: 16.*6.62 Ab rteqq4Teb 4!
Survival and Growth Data Day Y Z AA B 0
(0 1) 0 1 1
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Z.4 Z,5.% Z,5. 17_- 24.31 Larvae weight (mg) - A - B tO '1eie C.alcucla.tio d.qa t1.
Calculations and data reviewed 4
be I0
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Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant; Nontreated Test dates: October 8-15, 2002 Project number: 483 Revelwtd by: e L*-
CaoneatrUoa(%) Repi.te Itiiiainumberof FI.%nnber of A-?anweight B-Pa*+L"rvA Lavuewrigbit.h 0 Weight I aid"nii ba" C&rv Mean ivi Nd skttah)(ead.. of P tnd.a.i larvae 1a. (m1) LINht (,.n) - A-B of Iamt (mI) (%) "614 (Mt) Maam
)
A 10 20 14973 24790 9817 09817 Control 11 10 10 15 143 25020 9877 09877 C 10 10 15056 23680 8624 08624 D 10 9 14 817 24 110 9293 09293 E 20 10 14 940 24210 9270 09270 1098% F 10 10 15000 25 190 10190 10190 1000 09400 7.8 0.0 G 10 10 15 170 23620 8450 08450 II 10 10 14741 24,430 9689 09689 I l0 10 15 177 24 760 9583 09583 22% J 10 10 14710 23,750 9040 0 9040 2000 0.9204 2.9 2.1 K 20 10 15054 24050 8996 0 8996 L 10 10 14732 23930 9.198 09198 M 10 10 14908 24990 10082 10082 43.9% N 10 20 14 866 24870 10004 10004 0 10 10 14648 25020 10.372 20372 P 10 10 14855 24210 9355 09355 Q 10 10 14942 23970 9028 09028 72% R 20 10 14 886 25060 10174 20174 1000 0.9192 86 2.2 S 20 10 14665 22.930 8265 08265 T 10 10 15071 24370 9299 09299 U 10 t0 14925 25.160 10235 10235 100% V 10 10 14819 24610 9791 09791 1000 09952 2.3 .2 W 10 10 15 193 25230 10037 10037 X 10 20 14665 24410 9745 09745 Y 20 10 14950 235480 10530 10530 100% Intake Z 20 10 14 663 25560 10897 20897 AA 20 10 14714 1 25.120 10 406 1.0406 10279 .93 B
BB 10 1 10 El 15089 24370 9281 09281 1000 1 1 1 1 MSD - Minimum Significant Difference Dunnett's MSD value; 00936 PMSD = Percent Minunum Significant Difference PNISD: 100 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control ad beatament that can be declared sialistically significant in a whole effluent toxicity test On average, a significant ddiferce occurs for Environmental Testing Solutions. LC chronic toxicity tests when a toxicant reduces Pimephalcs growth by 19 6% form tie control (determined through reference toxicant testing).
-Dunnett's MSD value:-- 00882 Lower PMSD bo--und decliI5 b-yUSEPK(I Oth perentile)-- 9-4%
PAIStD: 94 Upper PMSD bound determined by USEPA (90th pereentile) - 35%.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboroetift for Pimephalea growth In chronic rcrerence toxicant tests USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Prograrn. EPA-833-R-00-003. US Envn, o a Protecton Agency. Cincinnati, OIl
Environmental Testing Solutions, LLC Statistical Analyses I 2LAmiu Flab G.cwih SawSWIV9,uTM*-7Dpa Gcuwg*
End Dow tols E03!v To" SamoubNiw 1.a swq u MVSTP DM~lt,1horp M.ocaaazig Repeat Cý-% 2 2 3 -4 D-Comntr 039127 0.91n 0.1624 0&9M9 10.91 0.92 1.0190 0.145 0.96" 22 0.9513 0-040 MM99 0.9191 439 2.006 2.M4 I.72 0-9355 72 OS902 1.0174 0.126 01929 100 1.=25 0.97 I 0037 0.3745 Mm M2 Ne" id Mal CVY/. N S.Sz't Crtdea 1630 Meansi &2 O.CzoIo 09403 UM0 0.9403 0.8624 01.917T7 6.216 4 0.9517 t.=00 1091 0.9400 0.9991 0.9400 0.1450 1.0290 7.15 4 0.001 2.410 00936 0.9517 2.000 22 09204 01.9789 0.9204 0.1996 0.9583 2.90t 4 0.521 2.410 0.0936 0.9517 10000 439 09953 10505 0."953 0.9355 1.0372 4.311 4 .1412 2.410 00936 09517 72 09292 1000 (1.9775 0.9t92 0.8265 1.0174 1.571 4 0.344 2.410 0.0936- 09517 10000 too 09952 10504 0.9952 0.9745 1.0235 2.292 4 -1415 2.410 00936 09517 t20000 A.x~lk.7 Tean smutdel Critdwa Ske.e Karl ShaproWilles Tcrzuitaza nmmal imibunoe (pO 101) 0.959660053 0.184 -0.2J77249.Z9 Badal, Teatnd.icae equal vwawý (p 0.30) 0.1354019431 6020119282 15 00631706I Hypaeea~l. Tat CI-ba O.DS) NOEC LOEC ChY TVJ MSDU MSDIJ MSBI MSE F.Pto6 Dumefs Test df1 20 '00 1 0.093566423 0099509636 0 004080117 0 003014643 0.205624431L 5.21&
Linear er3OLnao (90 Resnnpks) retial % SD 95% CL.4tip) k IC05 >100 ICIO >200 ICIS >2001.
ICO1 >200 09.
2C25 >203 IC40 '200 B 2CS0 '200 07ý 01:
04
- 03 M0.2 01
-01
.021 0 20 40 60 80 100 120 Dose %
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- I -
i
.~08.
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Environmental Testing Solutions, LLC Statistical Analyses Larval Fish GC*,oi sad SurvwaJ Test-7 Day Growth Start Date- 10AW2 Test [D PpFRCR Sample ID TVA. Sequoyah Nuclea Plant - Ixtake End Date 10/11502 Lab MD ETS-Eav Testing Solutions Sample Type: DMX-D*ssscge Monitoring Report Sample Date Proocol EPAF 9 1-EPA Freshwaer- Test Species PP-Punephala promelm Coin*cmnts Cono-'% I 2 3 4 D-Conurol 0.9817 09877 08624 09293 100 1.0530 10897 1.0406 09281 Tra*rform: Unt-ansformned I-Tailed Coac-%" Mean N-Mean Man Mum Max CV% N t-Stat Critical MSD D-Control 09403 I.000 09403 0 8624 0 9877 6 186 4 100 10279 1.0931 10279 09231 10897 6780 4 -1929 1943 00882 ALtxthary Tests Statutic Crtical Skew Kart Shapuro-Wilk's Test indicates normal distrbuton (p >0 01) 0877389908 0749 -0938263685 -0532798057 F-Test micateus equal variances (p - 0 77) 1435506105 4746834564 Hypothesi Test (-tad. 005)
Homoscedastc t Test indicates no signifcanc differences sqnlO-08-02data.xLx
Environmental Testing Solutions, LLC Page I otIS Chronic Whole Effluent Toxicity Test (EPAI600/4-91/002 Method 1002.0)
Species:' Ceriodaphniadubia Date Time Analyst Cient: -7V.g - 4 nayy-t ieb.r Test start /0,.O8V o z- 1q Facility: __
Test end 16-q.. Ilsciq NPDES #: -Tj- DOZct10 Dilution rearationinformation: Comments:
Diu Prep re(%) I. Zz 1 4.3.'c 12.ioo Effluclvoh= c(mL) I o4q.*-4.Bo *1-60 , 0 - 15i o Ddudvolum(mL) 54, Uo *
-1EA.6 zo 0 Test organism information: Test information:
Organism age: Z 2_
_ _ _ __* Randomizin template: %ICLL.'
Date and times organisms tO-'l* lrlcý -ro If'0 Incubator number:
"wereborn. between: 2.
Organism source: Io-Ot-o-,z Ae,4 YCT batch: i -Co4.Oz. -.
Transfer bowl information: pH = dqj. Temperature = ;R I Selenastrum batch: (465 10. o- .oz CONTROL Survival andReproduction Data Replicate number Day 1 21 3 4 5 6 7 8 9 10 1I 2
Young produced S Adult mortallty Young produced
__,____o
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- Adult mortality I I 1C
- '-1 4~ Young produced ~ ic c*_L Lh,: 1-- )4~
Adult mortalty L ___ t.-T jC1 IaLI j L1
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6 Toa
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pro produced uced mortalityl Young produced 1jJI-31j
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4i~ ¶ ILI XI r) i0 c.)2 TotL young produced a 7_ W *3C 2-9 2e Final Adult Mfortality ___uU - -* __
Xfor3" Broods , p- '-
Calculations and data reviewed:
Test Renewal, Feeding. and Incubator Locaton Informa=on Mon Tue Wed Thu Fri Sat Sun Date a-- low0 A1 1020- tljd 16:- 1 Time I~
'I* 121q Lz2&
sVa L _._ La( 13Z* .Z._
Shelf ILocation
..-.. 7. cL *L 1U7I Control information: Acptance crrt*na Summary of test endpoints:
% of Male Adults: , 20% 7-day LC50 I 1-7.
% Adults having 3' Broods: a_____
80% NOEC 10"
% Mortality: _ S20% LOEC ,_Io001 Mean Offspring/Female: > 15.0 offpringtf=fae k2.-. ChV >l*
%CV: 19. V _ _< 40.0 %_ _ IC25 >I______
-- - wtSA flfl4fl3, tiLt.
Page 2 o08 Species: Ceriodaphniadubia Client: -rVA . Date: ./A8.oe2 CONCENTRATION: 0M-/.
1 Survival andReproductionData I
Day Re nfmnber
______________ I I -. 1__'
1 Young produced 4 -9 0 (D 7 C
(J 2 Youn prouce - I. I_ IL_L- . 10 C",
17.
4 Young produced L1 ToA ult d mortality,
,3z Final Adult 3f 3 2S Fluxl Adult MoraityH so .3L
- 4~ ~~ -1%.-I
-C-Concentration:
%Mortality: 07 Mean Offspring/Female: . ,. (,,
% Reduction from Control: .
Calculations and data reviewed: 4L CONCENTRATION: Z Z.
I aJ4 aire
'WLund~ oducton D~ata 1 Re fica numbe Day I 2 h*1 I2 11s 1 6 7 8 9 I
Young produced I 0 ý n0- 0S 0 0 0 (D 10 2 *"o'*°* J-=A=J j.. s..&..n..produced.
Young -Ib U b*0 1.-0Co ) ,
L _ . l L__
o A_
Adult mortality Adult mortality
\ L.LI L_ *- . u k -I*
4 Young Adult mortality produced \~
4 Young produced IL 1 G) 4 C'-u.j a'$-..
Total young produced Adlmralt L- C FinalAdultmortaty Concentration:
% Mortality: IC/.
Mean Offspring=Female: 73179 I
%Reduction from Control: iS.3.
Calculations and data reviewed:
.,,BugE A.. 00futnons, LLC Page 31o~t 8 I. Species: Ceriodaphniadubia Client: -r*A.- aaQ* .. Date: /10.,0662.
CONCENTRATION: 'Survival andReproductionData
_Y_ 1 2 3 . - -0 1 Young produced t .5 7-3 0 cQ to 0~c Adultmorta* L.. L-. L.-- L- U L- L.- - L 2 Young produced M" 0 C2) o C Adltiorailty $_ T 3 Young produced Z L44 * . LL4 . .3 4 Young produced Cl --C)- C- I..~Z~..~
s A~dult Youngproduced ortalityL-i 1 (C) 1i t
1- L4 J J 1 C-
"I Adult mortal.ty _ _ CU 6 Young produced 5ii i, go Adu**mortality _ L. L. '- ' . t. u L.-.
7 Young produced Totalyoungproduced - 32 'C, 30
__3 .__ q 34 3S 30 Flna Adult Mortality L. L.-- L -- - L..- (. - = I!
Concentration:
%Mortality: 07.
Mean Offspring/Female: 32. !F
% Reduction from Control: - V-. 7o Calculations and data reviewed:
CONCENTRATION: .Survival and Reproducon Data
,_ 1 2 'Replicate number Day 1 2 1 3 4 5 7 s 9 10 I Youngproduced 0 C 0 00 0 "D 0 0 C 0 A _dultmortl _, , L '- Ui L-(--- I_ L(
2 [ Young produced 0 " C. r) r "0 Adult mortauity L, LK 3
4 IAdult Young produced mortality Young producedl qI
- Cc:I 0l'=~====V
~ ~ 1 ~ I Iti Adult mortaltLy L
= L T II, T F Adult mortaity - L.. 2_L I L L-L- rt 6 Young produced itl zc) I qG js{
__Adult mortaity VL LL '- lu __M_
7 1_Young produced - . -
Total young produced j3 i3Z 36 c3, 31 3-f13~34 33 30 (31 Final Adult Mortality~ J~
Concentration:
%Mortality:
Mean Offspring/Female: 3qV. I
% Reduction from Control: "2q.3 Calculations and data reviewed:
Environmental Testing Solutions, LLC Parry 4 ct X Page 4 at H Species: Ceriodaphniadubia Client: 7V9 -N /-'fO*van Date: /t 3A.4 P.
De*A, CONCENTRATION: Survival andReproduction Data Day 1 2 3 4 5 6 7 U 9 10 1 o mig p r o uc*e YAdultma 0)
L-0.- r) (7)'. * (3 0
- - L_- - L 3 Young produced (J s a -i:
4 Young produced A dol mortality Adult mortality L
C 0
k-0
ý.
1 N----
/*
I L 0 O1.
k-- LL-
" o --
L
- CD.J%&.ho'*O-7 Young6 producedj produced Young C j __
Adult mortalityC u i a d r e 5 Yong1on podued 1cntrtion Adult~~ItaCLy moralty Total young produced ?. *& 3 a 20 Z31 6-1 141 Final Adult Mortality .- .. --.. 7-L wa 6 7L 8 9 10 Concentration:
% Mortality:
- Mean Offspring/Female: .34.a
%Reduction from Control: *'21.0%
Calculations and data reviewed:
CONCENTRATION: j#io1.Survival Aduit mortailty IL- U. U U andReproduction L-*! Data !U Da 6 IYoung produced Adltmrtly K -
2 3 4T1
~~~~~~~Replicate 5
number_____
6 17 8 19 10 L - -- C- L, I- L-2 Young produced f) 0 3 3 p n C I
__ Adult mortality 1J~ LL~.- t L. Lju u 2 Young produced 0 S(1)L -S Adul mortality lL __ Z1U1 I-i V I {%J)1 4 Young produced 2 CI n~ C_-
5 Young produced N ( 7 2 5 015 Adult mortality U _ I ALjL __ U_
61 Youngproduced I9 rs.. /.L 1 1 12. jo 5 r).% 11I/
Adult mortality ~j j L--- - t, N'. - 1~t 7]_Youngproduced PP _____
Total young produced r 3o 2. 1 20 3 /(z'2 Final Adult Mortality L- L. L. *" VL U Concentration:
%Mortality:
Mean Offspring/Female: 27. "
% Reduction from Control:
Calculations and data reviewed:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalesprom elas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, UV Treated Test dates: October 8-14, 2002 Project number. 483 Reveiwed by: /-
CooeCetoution (%) Replicate Inaiial umber of Flatl oiuber of lara. A - Pla weight B - Nl + Larvae Larvwaweighl(rag) Weight I initial .omber Metseu sorv*l Mesto wevkl (mg) *t lla N. radioed" ki f
Larvae lMV) wel~bI (iag) - A- oaf larva* (mg) (.1 votaluaa(1 c (o%)
A 10 10 15 187 27190 12003 1 2003 Control H 10 10 14964 26910 11946 1.1946 1000 1.1346 8.7 Not agpileal" C 20 10 14945 26480 11535 1.1535
) 10 10 14 871 24770 9899 09899 E 10 20 14 873 24690 9817 09817 1098% F 10 9 15 106 25 220 10004 20004 97.5 0.9840 1.9 13.3 G 10 20 14922 24870 9948 09948 U2 10 10 15008 24600 9592 09592 1 10 10 15 145 26630 21485 2 1485 22% J 10 10 15063 26620 22557 2 1557 97.5 1.0454 14.0 7.9 K 20 10 15 162 25510 10348 20348 L 10 9 15 175 23600 8425 08425 Al1 10 10 15051 26960 22909 2 1909 439% N 10 10 14976 25880 10904 20904 1000 1.1023 6.2 21 O 10 10 15 156 25410 10254 20254 P 10 10 15076 26100 22024 21024 Q 10 '20 15282 26910 11.728 2 1728 72% R 10 10 14992 25330 10338 20338 1000 20848 11.0 4.4 S 20 20 15073 24470 9397 09397 T 10 10 14980 26910 22930 1.1930 1 U 20 20 14647 26 150 11503 1.1503 100% V 20 20 25185 25990 20805 20805 1000 1.1555 7.0 -I1 NY 10 10 15 152 27850 12698 22698 X 10 10 14645 25860 11215 1.1215 Y 10 10 14760 26610 I1 850 2 1850 100% Intake Z 10 10 15019 26190 22171 1.1171 100.0 1.0960 6.4 3.4 AA 10 10 15 193 25.710 10517 20517 212 10 10 15 120 25420 10 300 20300 MSD - Minimum Significant Difference Dunnetl's hISD value: 0 1662 PMSD" Percent Minimum Significant Diffiercnce PMSD: 146 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control Andtreanren that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occsr for Environmental Teating Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19 6% from the control (determined through l)unn-it's hISD value: 01175 Lower PMSD bound determined by USEPA (10th percentile) - 9.46./
PMSD: 104 Upper PMSD bound determined by USEPA (90th percentile) - 35%
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pisnephales growth In chronic reference toxicant tests USEPA 2000 Undertanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmettlal Prolaction Agency, Cincinnati, 022
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses lient: TVA Sequoyah Nuclear Plant, Nontreated
- st dates: October 8-14, 2002
- oject number: 483 Reveiwed by: A v
ýonccntrallon Replicate number Survival Average reproduction Coemded df Persmt reducdoa from 4 5 6 7 8 9 10 M) (offspring/female) vard (%) coa (%)
(%) 1 2 3 Control 32 25 30 13 30 29 30 29 28 28 100 27.4 19.6 Not applkable 10.98% 31 32 31 25 30 15 29 30 31 32 100 28.6 18.1 -4.4 22% 36 31 30 29 27 37 33 33 29 31 100 31.6 10.0 -15.3 43.9% 32 29 30 34 30 39 34 35 30 30 100 32.3 9.8 -17.9 72% 33 32 36 38 31 37 34 33 30 37 100 34.1 &1 -24.5 100% 34 36 36 33 39 35 20 37 37 41 100 34.8 16.4 -27.0 100% Intake 32 30 30 32 27 20 33 14 28 28 100 27.4 21.8 0.0 dItfll 101: MSD = Minimum Significant Difference nnelt's MSD value: 4.496 PMSD = Percent Minimum Significant Difference ISD: 16.4 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests ake. when a toxicant reduces Ceriodaphnia reproduction by 11.8% from the control.
nnett's MSD value: 4.413 Lower PMSD bound detennined by USEPA ( 10 h percentile) = 11%
16.1 Upper PMSD bound detennined by USEPA (90' percentile) = 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cer6odaphnla reproduction in chronic reference toxicant tests.
EPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-0)-003. US Environmental itection Agency, Cincinnati, Oil.
- 4. 0 Reproduction D-Con'ol ........ ........
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Environmental Testing Solutions, LLC Statistical Analyses Used for PMSD calc-ulatlon only .. Cerodaphala Survlvai and Reprodaction Test-Reprodtct-so Start Date.m l0/8102 Test aD) CdFRCR Sample UID TVA, Sequoyah Nuclear Plant End Duze 10/14102 Lab ID3 ETS-Env Testing Solutons Sample Type- DI)t.-Dwi rge MWauonrg Re-ort Sample Date Protocol EPAF 9 I-EPA Freshwicx- Test Species CCodap.inia dibta Commens Cone-% 1 2 3 4 5 6 7 a 9 1o D-Contml 32.000 25.000 30 000 13 000 30000 29 000 30000 29000 200 28000 109! 31000 32.000 31000 25000 30000 15000 29.000 30000 31.300 32.000 22 36000 31 000 30000 29000 27000 37000 33 000 33-000 29000 31.000 43 9 37.000 29 000 30 000 34 000 30 000 39 000 34000 35.000 30.000 30000 72 33000 32 000 36.000 39 000 31 000 37000 34000 33.000 30.000 37.000 100 34000 36000 36.000 33000 39000 35 000 20000 37.000 37.000 41.000 Transform: Untransformed 1-Tailed Cone-% Mean N-Mesa Mean Nfim Max CV% N t-Stat Critical MSD D-Comrol 27400 10000 27400 13.000 32000 19631 10 1098 20600 10438 21600 15.000 32000 13 146 t0 .0610 2287 4496 22 3 .600 1 1533 31600 27.000 37000 10.029 10 -2.136 2 287 4496 43 9 32.200 1.1789 32.300 29 000 39000 9 796 10 -2492 2.287 4496 72 34100 1.2445 34100 30000 38000 8114 10 -3408 2.2a7 4496 100 34000 L2701 34800 20000 41000 16.357 t0 -3764 2.287 4496 Awxdwaoy Tests statistic Critical Skew Kurt Kolmogorov D Test indicates non.normeal ditributon (p c- 0 0 1) 1.05*434725 1.03S -18 44358283 4 319992863 Bardees Test indicates equal vanaices (p -0 13) 3316535759 15.08631706 1 Hypothesis Test (1-taL. 0 05) NOEC LOEC dhV TU MSDU ,MSDp MSB MSE F-Prab df DuanetfTest 100 >100 I 4496034323 0164088344 8702666667 19.32962963 0001676433 3,54 sqnjO1-O8-O2data i
Environmental Testing Solutions, LLC Statistical Analyses Used for PNMSDackulatioa only Cerlodaplm Suval ad Reprodutcon Test-Reproductlon Sun Daue 10/302 - Test ID CdFRCa. Sample ED End Date 10/14/02 TVA. Sequoyah Nuclew Plant. Intake Lab ID: ETS-Env Tesng Solutions Sample Type- DMR-Dtscharme Monitring Report Sample Daze- Potneol EPAF 91 -EPA Freshwaim Tat Species CD-Ccnodaphn" dubi Comments Cozic-*/, I 2 3 4 6 7 a D-Con.rtL 32000 9 1o 25000 30000 13 000 30000 29000 30000 29000 2 000 100 32000 30000 28 000 30000 31.000 27 000 20000 33000 14000 23000 28000 Transform Unts-ansformed Cone-%. Meau N-Mean Mbean 3-Taded min Max CV% N t-Stat Critical WSD D-Coatol 27400 1 0000 27400 13000 32.000 19631 10 100 27400 10000 27400 14000 33000 21.344 10 0 000 1.734 4413 AuL*ixary Tests statistic Cntical Shapio-Wilk's Test midicaits non-normal distributon (p <- 0 01) Skew Kurt 0 755762453 0386 F-Test ndcate equal vartices (p - 0.76) -1 306076918 Z.667645544 1 28095234 6.54108572 Hypothesis Test (1-til. 0 05)
Homoscadasoc t Test indicates no significant Ciffe*encCs I
sqnlO-O8-O2data.xts
Environmental Testin, Solutions. LLC Page Ioatb Environmental Testin2 Solutions. LLC Faze I ox l Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephalespromelas Client: 7*VA Facility: .
NPDES#: r 17n,,6ur/I
- M26 S*6
-0 O
V-"r.ta C, Dilution preparationinformation: Comments:
MHS batch: /1).6_0_
Dfluatvolume(mL) "780
- 79. 64o1 2801 0 Test organisn;information: 2'1.* b)-. - Test information:
Organism age: "- 24-,phowr sa Randomizing template: _ _ED Date and times organisms 10 0 - 3 t Incubator number:
were born between: 12-30 o;o 1530 t .3 Organism source: AZ&* IV io-a1- -z- Artemia lot number 61*] o Transfer bowl information: pH = Temperature = Oven temperature:'0 Z C I _I___7.qt _2q.I 'C Drying time: d - o4 Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 0 1o-o&-o02. - )O _ _____
1 10t t- c09-- O IP2,*qSL I-ST,,
2 1 rI) z- l-2 5(
3 1o-1-oS. lolq 150,7 4 to- -o . lo n t -7o I G 5 10-13-.z1 o9*i io Ul0 6 I*0;.oz-. 00 Z_ 166 Control information: Acc e ent. Summary of test endpoints:
% Mortality: n -,%
- 20% 7-day LC50 >/cOo'd Average weight per larvae: I!. /S ( > 0.25 mg/larvae NOEC /_0_127 LOEC ChV I >Y/50*r, IC25 I___ /607_
jr~uv~fjiruidfl[3 1i es fll flhwihnine It (i I 2 Page 2 of a Species: Pimepha.(espromelas Client: T'i Date: /10. Ol'd I L)v --'ea~de
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A -Pan weight (mg) &46<~ -6 - - 1 1 Larv- (mg - wegh - - -
Calculations and data reviewed:~{
Comments:
Environmental Testingy Solutions~ VT C I Fag 3 Ot 8
Iage 3 Species: Pimephalespromelas Client: 7/ 5
~wo Date: i0.bd)6-~Z Day 4397~-, ...La IL.
- -q2- -/- -6 11 1
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Calculations and data reviewed:~
Comments:
Environmental Testing Solutions. LLC I r-Ige t~ at 8 Species: Pimephalespromelas Client: gpo-I,',j, h Date: /D.O.6'2z U V - 77Re 4,&k-d Survival and Growth Data Day j-p7"/.
Y Z AA BB 0
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2 1 I0 t O Io 4 / 1 10 -/0 5 /o to (0 10 6 /0 0 /Co /0 7 i' 10 /0 Id A -Pan weight (mg) It ON,14:f5 %-0 B Pan+Larvaeweight mg) 4.-w 0 l.I Z.1t Z%.qZ Larvae weight (mg) -A--B
-.B iI.* iAi11\ $1 I -
Calculations and data reviewed:
4-Comments:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pinephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, UV Treated Test dates: October 8-14, 2002 Project number: 483 Revelwed by: /" rUAV Coaltain.ioa (%) RqaUra, lariat number of Final number aflase A - Pa. weig ht B- Pa. + Larvae Lanae weight (mg) Weigh! /Iit.ia stmb.muir ,le a M weit (w1t) k /1 o edihs s [rtm Jarvia (Mag) weight (mg) A-0 of larvae (ag) (%) vat (%) to" )
A 10 10 15187 27.190 12003 12003 Control B 10 10 14964 26910 11946 1 1946 1000 1.346 37 Notapplia" C 10 10 14945 26480 11535 I 1535 D 10 10 14871 24770 9899 09899 E 10 10 14873 24690 9817 09817 10.98% F 10 9 15 106 25 110 10004 10004 91.5 0.9840 1.9 13.3 G 10 10 14922 24870 9948 09948 II 10 10 15008 24600 9592 09592 I 10 10 15 145 26630 11485 1.1485 22% J 10 10 15063 26620 11557 1.1557 97.5 1.0454 14.0 7.9 K 10 10 15 162 25510 10348 10348 L 10 9 15 175 23600 8425 08425 M 10 10 15051 26960 11909 I 1909 N 10 10 14976 25880 10904 10904 1000 1.1023 6.2 2,J 0 10 10 15 156 25410 10254 10254 P 10 10 15076 26100 11024 1.1024 Q 10 ,10 15 182 26910 11.728 1.1728 72% R 10 10 14992 25330 10338 10338 1000 1.0848 11.0 4.4 S 10 10 15073 24470 9397 09397 T 10 10 14980 26910 11.930 1 1930 U 10 10 14647 26150 11503 1 1503 100% V 10 10 15185 25990 10805 10805 1000 1.1555 70 W 10 10 15 152 27850 12698 12698 X 10 10 14645 25860 11215 1.1215 Y 10 10 14760 26610, 11850 1.1850 100% Intake Z 10 10 15019 26190 11.171 1 1171 100 1.0960 6.4 3.4 AA 10 10 15193 25710 10517 10517 BB 10 10 15 120 25420 10300 1 0300 OUtfil1 101" MSD - Minimum Signficant Difference Dunnett's MSD value: 0.1662 PMSD - Percent Minimum Significant Difference PMSD: 14.6 PMSD is a measure of test precision. The PMSD Is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole emuent toxicity lest. On average, a significant difference occuse for Environmental Testing Inake- Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19 6% from the control (determined through
.Dunncitt's hist value: .... 0.1175 Lower PMSD bound detennined by USEPA (10th percentile) - 9 4%
PMSD.: 10.4 Upper PMSD bound determined by USEPA (90th percentile) - 350/.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratorles for Pimephsalea growth In chronic reference toxicant tests I.
USEPA 2000 Undentanding and Accounting for Method Vanability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833.R-00-003. US Envimnmea Protection Agency, Cincinnati, OIl
Environmental Testing Solutions, LLC Statistical Analyses Lurva FUIRG"uw*t ad Swv*a1 TeM.7 Day Growtl Star Does 1042 Test G> 4F9FR SManla. 1D . A. SaquoyahMk Nu w I'laus FMdDSM 10/11M3 Lab 1D ETs-Ev, T~ts Soksooa Sinxp*TYpe- D1,lLD-hxw Ykenort R~epo Kam Dow I'usOcL, EPAF 914EPA Fmbswawa Tes Species: P?F.1kqsha promd CoasMaMI UV.Tresand
-Coo*.% 1 2 3 4 D-Ccautil 1.2003 1.1946 1.153S 0.9199 1098 0.9317 1 O4 0994S 0.9592 22 1.1485 1.1557 tin"4 0.3425 439 1.1909 I.0904 1.02S4 1.1024 72 1.1721 M.39* 0.9397 1.1930 100 1.1503 ]ADS0 1.2698 1.1213 Trasfnslo~n. Ustrukaforuxd1Ted Isotoni COmeA.% Mes N-M&R Mme M. MAUX CV%/ N tl.Saa+/- Ceilica YAW Mmtl 1-MM D.Cowoxl 1 1346 1.=00 1.1346 09199 1.2W03 1697 4 11346 LIM00 1091 0 99Q 08673 0984Q 09192 1.000 1.161 4 2.113 L.41 01t662 t.0744 09470 22 10454 09214 104S4 09425 1.1557 13990 4 1-294 2.410 0.1662 1.044 09470 43.9 1.107.1 0.9715 LI1M2 1.025 1.1909 6 17 4 0461 2410 0 1662 10744 0.9470 72 1.0941 09562 10941 09397 1.1930 11.050 4 0721 2.410 01662 10744 09470 100 11555 10115 1 1553 10805 12698 7043 4 .0.304 2.410 01662 1.0744 09470 A'UUr-YTests .- Statisti Crulc,.1 Ske. Kaon Shbtzui-Wt1ks Test jodisis cormai dsumberuton(p > 0 01) 0 937664621 0 984 .0 62160029 00033116-31 Bardletts Tat: tudicae eq"a vanances IpV 0 13) 1444132S02 1508631706 Hyotbeso Test (1-rail. 905) [4OEC LoEC CbV 721 MSIDU MSDp MSB N4SF W-Prob di Dunnett'i Test 100 N100 1 0 16618429 0 146472723 0 015595262 0 009509197 0.200275555 5.11 LUnesr Interpoladon (30 Ile~sampls)
POW % SD 95% CL(Elp) Skew 1Cos5 10352 ICIO >100
[IIt >100 10.
1C20 >100 09' IC23 >100 MC40 '100o 0.0 1C50 >100 07T ImdseatesIC cswnzte ICUsthan the lowest concawnnon 06 0
IX 0.3 0.2 at 00
.01 0 20 40 60 80 100 120 Dose %
Dose-Response Plot 1-tail, 0.05 level of signtircance
~08 2
to06 0
0 0
sqn_10-08-02dat-UI
Environmental Testing Solutions, LLC Statistical Analyses LArval F~sbGrowth wadSurviva Test-1 Day Growt Start Data 10/112 TeanI PpFRCP. SampleMn TVA, Seuoa Nuclear Plant - Intake End Dawe loll=10 LAba), ETS-Env Testing Solutions Sample Type: I)MR-Ducharge Mounitrng Report Sample Dame Ptntcol. EPA? 9 [-EPA Freshwater TentSpeciesi PP.Piriephales pituneWa Cowmram IIV.Treated CO&C-% 1 2 3 4 D-Contral 1.2003 1 1946 1 1535 09899 too t.1150 1 tl71 U.517 t10300 Tratisforin. Ustransformed I-Tailed CO.C-%. Mean K-Meza Mean MmS~ M"s CV% N t-Stat Czitkal MSD D-Control 1.1346 1.0000 1.1346 09899 1.2003 3.697 4 100 10960 09660 1"060 10300 1 1850 6384 4 0639 1.943 01175 Ansdl.ry Tests Statistic Critcal Skew Karn Shaputi-Widk'sTesttrdicaes normal dzsmbutiou (p >00 1) 0924149632 0749 -0 83582504 .0.054987614 F-Teatindicates equal variances (p - 0.591 1989268303 4746834564 Hlypoethesis Test (t-tal 0 (15)
Hnmoseedasnic t Test windicateno significant dififerences sqno-08-02datOx19
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Phnephlalespromnelas Daily Chemical Analyses Client: Sequoyah Nuclear Plant Non-Treated Test dates: October 08-15, 2002 Project number: 483 Reviewed by: C,-
Concentration Parameter Da 0 Day 1 Da 2 Day 3 Da 4 Day 5 Day 6 Initial Final Initial Initial Final Initial Final Initial Final Initial I Final Initial Final pH1 (SU) 7 82 7.32 7 71 7.25 7 92 7.58 7 97 742 7 87 7.50 7.841 7.79 7 96 7.74 DO (mg/L) 7.8 7.1 81 6.9 84 7.2 81 69 , 7.8 6.9 78 76 7.9 7.7 Conductivity_ (mhos/cm) 294 302 314 312 301 304 314 Control Alkalinity (mg/L CaCQ) 61 62 60 Hardness (mg/L CaCQ) 82 86 82 Temperature CC) 244 24.8 242 25.0 246 24 9 246
- 253 243 250 24.7 248 246 25.3 PH (SU) 784 7.31 7.83 7.20 7.86 7.41 794 7.36 7.90 7.40 7.89 7.78 7.82 7.70 10.98% DO (mg/L) 7.8 7.1 82 69 8.2 7. 80 69 80 Conductivity (pmhos/cm) 283 288 301 20 6.9 7.8 7.7 80 7.7 Temperature CC) 244 24 8 245 250 245 24.9 247 253 244 25.0 24.7 24 8 24.7 25.3 PiH (SU) 7 83 7.33 7 82 7.24 7 84 7.40 7.93 7 33 7 87 7.43 7.87 7.74 7 83 7 69 22% DO (mg/L) 78 70 82 8 6 68 8.0 66 7.9 7.7 80 7.7 2% Conductivity (Iimhos/cm) 270 - 271 286 281 6,828428 Temperature CC) 24.6 24 8 24.5 25 0 24.5 24 9 247 25.3 244 25.0 247 24 8 2481 25 3 p11 (SU) 7 80 7.33 7 80 7 17 7 81 7.36 7.90 7.32 7 83 7.401 7.82 7.76 7.83 7.72 "43.9% DO(mg/L) 79 7.0 82 683 65 8.1 6.9 80 65 79 7.7 80 7.7 Conductivity (itmhos/cm) 24 261 259 254 256 263 Temperature CC) 24 6 4.7 24.7 24.7 2.3 24.5 250 24.7 24.8 2487 25.3 pH (SU) 7.62 7.32 7.76 7 21 7.76 7.38 7 85 7 30 7.78 7.35 7.77 7.73 7.80 7.70 72% DO(mg/L) 7.9 7.1 82 6.8 83 6.5 81 6.7 80 65 7.9 7.6 8.1 7.7 Conductivity (irnihos/cm) 214 215 224 222 220 222 226 Temperature CC) 247 24 8 249 25 0 247 24.7 253 245 25 0 24.7 24.8 248 25.3 pH (SU) 7.701 7281 7.74 7.19 7.72 734 790 7.30 768 7.36 , 7.66 7.741 7.781 7.71 DO (mg/L) 80 7.2 82 69 83 64 811 69 8164 7.9 7.6 8.1 7.8 Conductivity (lirnhios/cm) 182 8 192 11187 _188 191 100% Alkalinity (mg/L CaCQ) 61 63 62 Hardness (mg/L CaCO3 ) 72 74 100 Total Residual Chlorine (mgfL <0.10 - <D.100 U.10 Temperature CC) 25 0 24 8 25 0 24.7 24.9 24.7 25.3 24.5 25.0 24 8 24 8 25.3 pH (SU) 7,74 7.37 773 7.21 742 7.38 7.84 7.34 7.60 7.36 7.65 7.72 7.81 DO (mg/L) 8.2 721 8.31- 6.7 8.4 - 7.2 0 6.9 8.1 6.4 8 0 8.1 Conductivity (ttmhos/cm) 18118 195 190 183 185 100% Intake Alkalinity (mg/L CaCtQ) 62 62 57 Hardness (mg/L CaCO) 86 808 Total Residual Chlorine (mg/L 0.10 Temperature CC) 252 24 25425.3
e.age 5 t Species: Ceriodaphniadubia Client: "7"Vt - _6uv4*
Date: /09.6;7.
Full-strengthChemistry: Ou.t-'dJt / D Parameter Sample Number: Control Batch:
1 121T 3 pH
-(S.U.)" Gq.--o "-1.1z.. -I.a 1.9- 7.2 DO 6.0 (mg/)
Conductivity '1-7Z (ghos/cm) 167 Alkalinity (mg CaCO3 /L)
Hardness
-7q (,96 1- o (mg CaCO/L) "100 *'z.
- Z.
-le,I0.
(mg/L) 4 L0. 10 L I Collection start date
- 10. 0(0,.o 10-0*0-2 Collection end time IZo Grab or Composite (duration)
Temperature (°C) upon receipt 0.3paL 'C. z.q "C Physical characteristics -tan, P.ALe C4Car.. C.LeA vZ.
Dates sample used I/036oz / 0.lo.oz /.10.I Z.
f0.I1z/. ,z ETS Project and Sample numbers O02 Oo S3. o4t C.ZIOIO. 0* 0o'lo01z, 0"(f I
- ____________ L __________
-- :: rage f)ot z Species: Ceriodaphniadubia Client: -TVA Date: /o.o6.* z Full-strength Chemistry: "*-.k.
Parameter Sample Number: Control Batch:
'I 112
- I 3
pH (s.u.) q. Wi ý6 0 DO (mg/L) .04, Conductivity !gi (pnhos/cm) "Iq 'z.
Alkalinity (a2_
(mg CaCOG 3 L) 5,1 Hardness -Z. io g-2..
(mg CaCOG 3 L)
Chlorine 8-oa
- 0. .to (mg/L)
Collection start date o 62.
Collection end time Izq 2__
ola650 Grab or Composite
'ý- ~AUuL (duration)
Temperature (°C)
CýLR&POS Mte
.3"C upon receipt 0.00T z-q '"'
Physical PAi LC characteristics ce.ee TAMN CLe.' iL
- .oa.0 CLeJ'&..
Dates sample used lvo.'.o 2 /0.o-o.z Io. /z-0z
/0.0 9, .o /01.0
-I- z /0.1q.a .
ETS Project and q83 Samole numbers 040o0.06*" 011J~o. ioS Irr7 rini Sample numbers o2tcoe4) IC? IcLri,Ifr7 9tu7.
-..- *A* A JLXauug UtUioU, aLul "UL
.Page 7 ot 8 Species: Ceriodaphniadubia Client: _774A- 5#&9b Date: 0AM
cugauinLJLU Page zsot s Species: Ceriodaphniadubia Client: 7%'7.5f) 4)
Date: 6
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphniadubia Daily Chemical Analyses Client: Sequoyah Nuclear P1ant Non-Treated Test dates: October 08-15, 2002 Project number: 483 Reviewed by: v
. -. Reviewed by: Ck Loncentration irarameter Da 0 Da Da 2 )a3 Da4 Da I nFinal l IInitial Final IInitial Final Initial Fia Initial Final Intti 1i Day 6 Iptl (SU)1 7 82F Intial Final fl bt 7821 7.71 7891 792 8001 797 80 787 __7911 _ 7.841 I MI-u fIII!*LJ 78 821 81 84 8.4 8.11 8 8 7ý.8 Conductivity (pmhos/cm) 7.71 7. 0 Control Alkalinity (mgIL CaCqA 294 30314 61 312 30130 62 F!D5 Hardness (mgIL CaCq) I1 82 86 Temperature PH (SU) (C) 244 249 24.3 24.5 25.0 24.5 24.5 24.9 246 25.0 W 7841 7.75 783 7.7 786 802 7.94 I 10.98% DO7(mWL) 807 7.90 7.921 7.89 8.10 781 80 82 82 81 3 19 Conductivityp------i82783 80 288 301 294 290 293
_______Temperature rQ 245 249 244 253 246 20 24.7 252 245 249 246 250 PH (SU) 783 776 789 7842- 0 7.931 806 7 791 787 22% DO(mg/L) 8 11 78 80 82 84 8 81 80 Conductivity (pmhoslcm) 7.9IT 8 270 271 286 284 277 284 Temperature CC) 247 249 , 244 25.3 246 250 24.7 252 245 249 PH(SU) 246 250 7.80 7.74 780 7901 781 802 790 805 783 791 782 8"13 DO (mg/L) 79 801 8.2 8.4 83 Conductivit 8 . . 0 79 79 8 o 4
- 247 261 ___
Temperature CC) 24.7 24.9 214 259 254 - 256 253 24.7 247 25,2 245 24.9 24.6 D SU 25 0 762 77 - 7.7 7 91 7.76( 800 785 805 7.78 7.94 7.77 8 13 72% DOmI)7.9 80 82 84 83 821 81 841 801 7.9 7.9 Conductivity (pmhoslcm) 214 215 224 8.4 222 220 222
________Temperature CC) 24.91 24.9[ 24. 25 3 248 250 PH(SU) 7.70 7761 7.74 787 772 797 24 7 252 24.61 24.9 '24.7 2 7.90 768 766 8.112 DO (mgIL 80 8.01 82~ 851 83 82 81 8481 78.1 7669 8.40 Conductivit mhos/cm 182 183 192 19 187 188 100% Alkalinity (mg/L CaCQ) 61 63 62 Hardness (mg/L CaCQ) 72 74 100 Total Residual Chlorine (mg/L) <0 100 10 <0.10
_Temperture CC) 25.3 24.9 249 253 24 8 25 0 247 2246 24 24.71 250 pH (SU) 7.74 7.751 7.731 786 742 7.91 7 84 7 7.60 DO (mg/L) 8.2 8.3 8 8 07
-- 88.1 8.0 80 8 Conductlvity (mhoslcm) 1 181 195 190 183 185.
100% Intake Alkalinity (mgJL CaCq) 62 62 57 -
Hardness (mg/L CaCq) 86-- 80 82 Total Residual Chlorine (mg/L) <0 10<0.10--'-'0 Temperature eC) 25. 249 24.81 25 3 24.7 25 01 247 252 246 24 91 24.7 25 0
Species: Pimephalespromelas Client: -I' _ptry: tt-n ms- Date: /,0. O6Oz Full-strength Chemistry: (~q-fotL 101 Parameter Saple Number: Control Batch:
__________ 1 2 3 *j6.q.*2 Io-cfVOz-. t10V-Q2 pH (S.U.) I.q ,/- 7G .X ".7 -,t*
DO .. R.1.3 ... 1.A.0 1, (mg!L)
C onductivity je l - '12 - 18 -L Alkalinity (.2- GO (mg CaCO3fL) ( (.3 1_ _OZI Hardness -7q &C?( -F2-(ma CaCO 3/L) /00 Chlorine L6.IO ":6,10 0..1I (mg/L)
Collection start date .
Collection end time q Grab or Composite ..
I1ho2*
(duration) C,-*Co SP_ Cjaft't*
Temperature (0C) O.3*C upon receipt 0._"_ __.___._"
Physical PaLO PAAte_ PAALe.
characteristics 'ta"r"-. "r* "I CkeUart cLe4o, c? v 12 Dates sample used /4.06,0 /-5 .10.02 iO0.Z .02.
ETS Project and qIa3 o7 Sample numbers 021Vo6.Oq 021010.03 o210120-
Species: Pimephalespromelas Client: "T-A.- , L.a.. h Date: / ,d. 6z f %/
NOK"-rc..eATe-b Full-strengthChemistry: ,..
Parameter Sapie Number: Control Batch:
1 2 3 /0*.j2 1 10.o0.o7- 1o'11-67 pH pHu . -3
. -7 q -I,qz . G)
-J J .8 7 -1 q,
- 7. 6 (S.U.) I.h 1.o T DO -~
(mgL) "____
Conductivity Iiso (Wrxhos/cm) / ____ ___
Alkalinity (mg CaCO3IL) M L I_ (40 Hardness (mg CaCO 3/L) 002-Chlorine (mg/L) ___.__ ____. _ ______
Collection start date o- I* I- z Collection end time O5s 7 Grab or Composite o *-*.*yz. ,
(duration) *6 Co Rprv. *CPn'SIre Temperature (C) C upon receipt Physical FA.
f#Le- Ae characteristics -Mf,-3 Thp ctu, CLEAZ.. C"ePl Dates sample used o.00.0t /0!O.oO0Z *.12.0Z ETS Project and Sample numbers 02A60 a.o05 OZICo.O03 OZioI.0
P'age 7 o t 8 I
Species: Pimephalespromelas Client: 7 -5 19-LhOMrg~eTeb Date: -1666-62Z
Species: Pimephalespromelas Client: TiA- .e~aoguah Date: /-*6.d2 Da 34 5 6 Concentration Parameter
- 1. 34.1164 -;1-?~~~ *:Vq CONTROL PH (S.U.) 1 DO (m/n 1 0.i e 1--5 .g -A 1.
Conductivityq zOL3, (Imoslcrn 312301 1 Temperature
____ __ (CC) 2S. -4 0z. zqj--1 Zq4-9. 0 s
-. n--9 ý4 PH S.U.)
e.) . 9.6 3.
DO (m L. 610
/0.96 .4 Conductivity Z Zat -3 (pmhos/cm) -ZqL 2C1o Temperature .7 7 1LI *- 41 23 PH(S.U.) a Iý- ~ -483 6.0 .Ci (9-6~
DOm
- 1. Coductivity hos/cm)
Temperatuure zL4.1 F2s- Z. 11 2q.L(
DO (mgL 6,1 IF 9 .q0 q 97 L,13 Conductivity 222 S Ignos/cm)
Temperature -zq,4 2'. 2q.5 zq.~ I zq DO (mW)
/06/. Conductivity Temperature ZZ.7 Z* 21. z. -zV4f 2.
IOCJA C hos/cm' L)
- 5. t. Z(.~ -ze.00 ZY.~
Temperature r..i Tt1 Fnl zq- 2 J,q.I (cC) V- e )n 11
' .:;(,l a .111 I I 4 PH (SU. j~kiII4 B-ý Viq in'-
T-I+Inl rinal in L U
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pinephalespromelas Daily Chemical Analyses Client: Sequoyah Nuclear Plant UV-Treated Test dates: October 08-15,2002 Project number: 483 Reviewed by: C-'
Concentration Parameter Da 0 Da 1 Day 2 Da 3 Day 4 Day 5 Da 6 Initial Final Initial Final InitialI Final Initial Final Initial Final Initial I Final Initial Final pH (SU) 7.85 744 7 83 726 7841 753 789 741 789 7730 7.771 7.31 7.92 766 DO (niWL) 80 73 80 7851 7.11 8.1 7.1 7.71 64 7.71 7.21 80 7 Cnrl Conductivity (1imlios/cm) 291 291 32302 296 301 313 Control Alkalinity (mgL CaC q) 61 62 60 Hardness (mgIL CaCq) 82 86 82 Temperature eC) 245 25.1 246 25.1 244 24.7 244 252 244 2491 24.7 24.7 246 25.2 Pil (SU) 785 7.40 780 722 782 7 57 7 89 742 7.88 7.341 7.73 7.29 790 768 10.98% DO (mg/L) 80 7.3 8.1 7.0 85 7.1 82 70 7.7 641 7.7 7.1 78 78 Conductivity (itmhos/cm) 284 -285 ý 297 295 290 2-95 Temperature eC) 246 25.1 247 25.1. 245 24.7 2451 252 24.5 249 248 3069 24.7 249 2 Oil (SU) 7841 742 7.76 722 783 752 7891 7.38 786 734 7.72 7.28 783 765 DO (rg/L) 801 74 8.1 69 8.5 7.1 82 68 7.7 66 78 69 7.8 7.9 22% C o nd u ctivity (p m hos/cm ) 270 27 0- 284 2 81 I 275 2 82 -- [
. 297 Temperature CC) 246 25 1 24.8 25 1 24.5 247 245 25.2 245 24.91 248 24.7 249 252 pH1 (SU) 782 7.37 7.79 723 7.81 752 788 7.36 784 7241 767 7.28 7.87 SDO(mgL) 763 80 7.3 82 68 8.5 70, 82 6.8 7.7 661 77 6.91 78 78 Conductivity (pmhos/cm) 248 246 260 26 252 257 277 Temperature CC) 247 25.1 248 25.1 246 .5 252 245 24.9 24.7 247 248 252 pH (SU) 780 736 7.76 7.29 7.79 - 7.51 7.86 7.35 781 7.241 7.65 726 784 762 7DO (mg/L) 80 72 8.2 . 69 8.5 70 8.1 69 76 661 7.7 6.7 7.9 7.7 Conductivity (pmhos/cm) 214 215 223 1 220 224 237 Temperature eC) 24.7 25 25.1 246 24.7 24.8 25 2 24.5 24.9 24.7 24.71 248 25 2 PlH (SU) 7.59 7 34 7.72 7.26 7.73 7.47 7.82 7 37 7.77 7.20 7.59 7.251 7.79 76 DO (mg/L) 80 74 82 68 84 70 80 66 76 6.5 7.7 681 80 7.7 Conductivity 180
-Hmhoslcm) 182 19 187 190 20 100% Alkalinity (mgIL CaCOq) 61 63 62"11111101311111 H ardness (m g/L C aC Q ) 72 '100- 74 Total Residual Chlorine (mg/L <0 10 0 10<0 10 Temperature eC) 249 25,1 251 25.1 246 24.7 248 252 24.5 249 248 2471 249 25.2 pH (SU) 771 7.41 7.72 7.20 7.72 7.53 7.78 735 7.74 7.24 7.55 7.331 57m 7.66 7.64 DO (m/L) 8.1 7-3 81 __-8.4 0 791 67 -7.8 66 7.7 66- 8 7.98 Conductivity (limhos/cm) 180 180 191 186 183 ~ .187 186 100% Intake Alkalinity (mg/L CaCq) 62 62 57 Hardness (mg/L CaCq) 86 80 82 Total Residual Chlorine (mg/L <0 10 <0 10 <0.10
[Te perature (C) 2541 251 252 2511 246 247 246 252 245 2491 252
Page 5 ot8 Environmental Testins! Solutions. LLC Species: Pimephalespromelas client: hrV/ Date: //1.-6-6Z Full-strengthChemistry: 0,4w-L 16 1 Parameter Saple Number: Control Batch:
1 2 3 0. l 00Iq.Z0o. tf-f-7O pH 1I (S.U.) I,_______
DO O -7.(o (mg/L) .
Conductivity 117I (ginhos/cm) ____ .___ N____ ___
Alkalinity (mg CaCO3/L) _ ___,, _____ _____ _ , _
Hardness -7q 7z2 100 (ms CaCO3 L)
Chlorine I- .]R .1-0 .10 -<o,10 Collection start date o.'o-02 . c0Z-O-L 1O-lO-Oz_
Collection end time o00l] aOVti Grab or Composite _.-ovz . -_.
(duration) CA)Q!e COircITE MDttdSirM 0.3,c O.C -ZC,"
Temperature CC) upon receipt Physical -lt IPAt e tALe characteristics .fl~n, -Mm 7"r Dates sample used fo.d.toz /o.io.oz iO.12.oz f0.VOZ~ , O.1(.07- /o.13-. z ETS Project and 430 Sample numbers OzOD0.1O04 OtIOiO.05 0Z/012.C7
JU" vim I -
Species: Pimephalespromelas Client: " Date: / .
Ft C Fufl-strength Chemistr:3'* -621 Parameter' Sam ple Number: Control Batch:
1 2 3 It.0)* .oZ 1I.o 01 -Il-6Z*
pH . 77.- -- -7.'7q 1 _____
(S.U.) I___
DO .I g.q . E3.0 - ..
(ng/L) "
Conductivity e ) 1 I19 (mnChos/cm)
Allcalinity &
(mg CaC0 3fLQ to2zt a 2 Hardness (P7 I (m CaCO3 IL)
Chlorine LO. 10 46,10 1 -O, I0 Collection start date I0.OLuoZ 10-08.0Z fo40-"7 Collection end time 0 & J0o* Izq -L Grab or Composite )A7jj ',nJ#L (duration) caqpe caO-mSI re.
g, Temperature (CC) o(.3'C O. 9 "C 2A. C_
upon receipt Physical PAo LE AA*-e characteristics Th.A,0 -3 T
Cb*art. r-LgAP- c.LeAP_
Dates sample used /1.06.02 /O./D.OZ /.D12.62
/,0 .oz 0 /6.I o 1/O.-I'/"02 ETS Project and q83g .3 Sample numbers OZ100C.0dO 01//l°0.0 O7?Jt2"OI
, ... ,* .rag, g7 01o Species: Pimephalespromelas Client: "%- o Date: /M,- h-62 Daily Chemistry: V V '7-r~eLCaG Concentration Parameter 0 D1 2 CONTROL PH (S.U.) 5q.8"5 1.83 'aq 1 -'5 DO(mog/) dr. l 6.0, V4.0 (ghsm e Li dt q 221 Conductivity Temperature Tmc) pera, .r A 2 IA Z -2q., ' jq-7 (gmhoski) */bW3
- DO (mglL) F- 0 4.1I .o 2 '1 (Q Conductivity zqc) f zI ZL.5 zq, 7 Temperature 2q.I Ze. 2. \ i pH (S.U.) "c. *- 3- 2 5.-2
-DO (rag/) 2 Z U.q (0A"* *b "U2- '.
(0uhscm)
Conductivity 0' 18Z I V[ . q.
,(Wnhos/cm) 100 -4 pH (S.U.) -' ql-, z .. o," q.55 Temperature z, q.
zq7 ( *-1 2q.z, z5."7 Conductivity
/6L" ( ohos/cm) .. t Temperature ".2 T e mp _-_1 e rature 2 4_ z. . 9 2 J .1 Initial I Final I Initial II Final I TnitFl II II 1';iqI I!
I Fin Initiaol -pin
LALIUUX0UIUnons, LLC Pg 50 Species: Pimephalespromelas Client: 477A~LO/
Date: pjv~
Concetai osc 0 '1::
334o1 16 Z1 Codc ti2vity.Z (I..
tq. z .217 2,71.
(Imos/cm)
Ic30 31 Temperature (4 '*2 COC) 7-.2 L. ~ 2z g'z Pniia
.- g -. ( Z .Z Fia Iiil ia iil ia Iiil ia
'92 /.
//65" */
tool7.
Page (5 Environmental Testing Solutions. LLC Alkalinity (EPA Method 310.1)
Matrix Water, MDL = 1.0 mg CaCO3/L Analyst L Z Date analyzed L )b-!J5-O Titrate samples to pH = 4.50 S.U.
Tfrant normalt nd mu*l*eermina on:
pH of Normality Normality (N) of H2SO4 . pH Factor or Multiplier Delonized Titrant check Begin End Total (5 ml NazCO3 x 0.05YE -(Nx 50000)/100 ml sample water reference standard ml ml ml - 0.25/E -Nx 500
- 4.5 S.U. number number - - (E) (acceptable rme - 0.019 - &.022) n1' .,- o.a - 0.3 *-n1 Laboratorycontrol standard:
Reference True value Sample Alkalinity (MV) % RS = MV I TV 100I standard number (TV) volume Begin End Total Multiplier , (mg CaCOi/L) (acceptable ra-ze (m9 CaCO/) (mV) ml ml ml - 90 to 110%)
10k3 PO 11" 100 100 ' to AA CM
- 12.q Duplicatesamle precision:
Sample Alkalinity %RPD =
Sample Sample ID volume Begin End Total Multiplier (mr CaCO1 /L) {(S- D)/[(S+D)i2]) x 100 number (ml) ml ml ml I 1IOoozax MrtH5 O z 2.,1 ZI.;z ki. 3 q" ,7.*;
(acceptable range -110%)
Duphcate t 2.2. . 4. D L.co'7'
)D.07-02~.
1 0 --. 2- 35 I.
Matrix spike recovery:
Reference Spike value Sample Spike alkalinity (A).
standard number (SV) volume Begin End Total Multiplier (mg CaCO,/L)
(mg CaiCO31L) (ml) ml ml ml I* 100 '1040. 11.3 9.9 q1o Sample alkalinity (B) Measured spike value (MV) % R =MV /SVx 100 (mg CaCO3 IL) MV = A - B (acceptable range (mg CaCO3/L) -75 to 125%)
Sape mealurenents:-__
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO/L) 10- 05-Ov z.z , ,.7- (11 l o7.oZA 0 _.z Iaq (...?
________ 13.A tq5 G.) _ _ _ _ _
iJ.o2, Z IO. _6.o ,. (10 io>. L3.07-. *38,, .' Uz* -,,3 I D.-0 OZ. ,_,_a 3 6.4 qJ.9 3." 35
)0.0.o71- 1 6"mt-_ W_ 0_0.. . Z-. - _ Z Ot Reviewed by: I Date reviewed: I 1o-Ze-62-7 I
Page e_
Environmental Testing Solutions, LLC Page 3 of: Ii i1I Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO 3/L Date analyzed to.v01- Titrate samples to pH = 4.50 S.U.
PH of I Normality -- 0 o'll 0%04 pH Factor or Multiplier L Delonized Tltrant check Begin End Total (5 M - 10.25/E--
Il Na .2 Nx 50000)1100 nl sample standard ml ml ml water reference
- 4.5 S.U. number number (__) (acceptable range - 0.018 - 0.022)
Laboratorycontrol standard:
Reference True value Sample Alkalinity (MV) % RS MV I TV x'100 standard number (rag (TV)
CACOAL) volume (ml)
Begin nt End ml Total nil Multiplier (Ing CaCO/L) (acceptable range
=9OtollO%)
100 100 -ZA. oi.c cljq Pi9 Duplicate sample precision:
Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO31L) [(S- D)/[(S+D)/2I x 100 number (ml) I i ml ml (acceptable range - 4-10%)
s
- ozlbo.o_3 l ZaL.3 43 to. q
-00 Duphicalc b D -g7.
Matrix spike recovery:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO31L)
(Ma CaCO1 /L) (ml) ml ml mi 50 Ioo .,z, t5 " 0#6.
Sample alkalinity (B) Measured spike value (MV) % R =MV ISVx 100 (mg CaCO3IL) MV[V = A - B (acceptable range (mg CaCO,/L) 75 to 125%)
Sample measurements:
Sample volume Begin End Total Alkalinity "
Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOVL) ovto,.o4 6Qt ,., -.. 10i III L, -. 1 z .q a CSV _ 0o4 3 -;__.3 134,, 6.3 (o_
tr s -Jt tIr.4I '1 _ U_z3~
ozlitl.0' OR,,,_ .3 sq z.z- S.1 __ ..
PPAC- (P-19 oz.g ,i- a ____
___AC__ Lt - - 4 qq L 0I
_______15 .0 q. q
__________q. .1q~ 0 LJrei Reviewed by: 41Z] Date Dt reviewed:
rvee:[lii~i I I0.Z'.OL_.
Environmental Testing Solutions. LLC "Page 82.
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO 3/L Date analyze ii I T71*ran noa andmuplie determination:
Titrant Normality check Begin End Total Normality (A) of EDTA pH Factor or Multiplier reference standard ml mnl l = 0.2/E (Nix 50000)/50 ml sample number number (E) (acceptable rang, - 0.018- 0.022) Nx 1000 Ip.osb IN650cjz.' 0.00 10.0 10.0 0.010 ZO Laboratorycontrol standard:
Reference True value Sample Hardness (MV) % RS = MV/TV x 100 standard number (TV) volume Begin End Total Multiplier (MC CaCO,/L) (acceptable range (rg CaCOaL) (ml) ml ml ml - 9otoll0%)
ir*.oJ 40 50 1.0 t2.1 20 qzI Duplicaatesam le precision:
Sample Hardness %RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCO,/L) I(S - D) I[(S+Dy211 x 100 number (ml) ml ml ml (acceptable range - 10%)
1O.01-02 l-7 Ar 2.l L I4.9 Zo s '908 Q.
l00 - Duplicate 4 l, , o 4 D go0 .520 Matrix spike recovery:
Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOiIL)
(mg CaCO3 L) ml ml ml ml l 4.0
- ~J Z33 U5 to, l z C) 130 Sample hardness (B) Measured spike value (MV) % R = MVI SVx 100 (mg CaCO3/L) V= A- B (acceptable range (mg CaCO3/L) -75 to 125%)
qo -40 .0o -.
Sample measurements:
Sample volume Begin End Total Hardness Sample number SampleID (ml) ml ml ml Multiplier (mg CaCO3(L)
(shoulbe-o mg;CCOa) 60 0.0 0,0 O.O ZO D 10-oz-O IiS zg,!neq3 I __ ___
10-0g[-b2 12-4.6 .37,o q.), eq 1ot.I-o2 tA 37,0 Ubjll 44.1 69 I0.1l.oz _4.5" 4S.4.L_.1 _ _,
in. 13 -*) Z. o0o ,4.0 q 60 to. 0*-0-2_ SSU-) 140 q* ** lglqq OZ.OS.ol0 S .- J q.o L-3 tt. 7.ZS Note: If > 15 ml of titrant is used, sample must be diluted. Reviewed by: Date reviewed [ 10O-.-g- 0 -- ]
".Environmental Testing Solutions T.T.1' 1 k,*
age 6q S... .... . . .Page j
-of -=
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCOC/L Analyst IO-IZI.
Date analyzed L L Laboratorycontrol standard..
Reference True value Sample standard number Hardness (MV) RS =MVI TVx 100 (TV) volume Begin End Total Multiplier (mg CaCOV/L) (acceptable range (Mg CaCO3fL) mL ml nml In -90 to 110%)
1 40 50 1 0.1 , a-v 20 10 DuDlicatesamnle nr~renn -o-Sample Hardness Sample Sample ID volume %RPD =
Begin End Total Multiplier (mg CaCO)/L) number {(S - D) /[(S+D)/2]) x 100 (ml) ml ml ml (acceptable range - 4 10%)
02i .0& 5GPt I1 I 6.lb,, ZO s Z.
,t Duplicate 60 5. 5 . ZO D
-L Note: If >l1m] of tarmt is used, saple must be diluted. Reviewed by: II,,"I]
= 9 Date reviewed
-L..-C-Z--
[ 02- I]
Environmental Testing Solutions, LLC Page qg Page .ofI /
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter Accumet Model 25 pH/Ilon Meter Analyst [Z'0 III] Iodide reagent:
Date analyzed /Qj). Acid reagent:
Slope:
Calibration:
4 rt ~ A 0.10 mgIL I 1.00 MgfL Reference standard number Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory controlstandard:
Reference standard True value (TV) Measured value (MV) % RS =MVI TVx 100 number (re/L) (mgfL) (acceptable range - 90 to 110%)
0.50 (
Duplicatesample precision:
Sample Sample U) -Sample characteristics Residual chlorixie %RPD = [(S - D) /[(S+D)/2J} x 100 number i(ag/L) (acceptable range -1 10%)
02! tt~ h I S ~ dc-------------------- -
Duplicate * ,D z0D.0 Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mg/L)
- ..~~~~~~~~~~~,*,/,,,,
,z ,:,,;;.szc>.,,,,,, ,,,.,,,, * *--
spsre-; Blank (should be = <n0.e10 mgusL)
Note u'"o r..
02 abo06-o03 contr ozolFP stand ard:52d O2DbebS.0Z- I~ncLuc~nC(O.S6A poitcps ~ c~o1-' 6dn ai ooe.oD4 7T £01r [lzj& 1 Ai Nate- All samples were analyzed in excess of'EPA recoimmended holding tume (15 minutes) unless otherwue noted.
Laboratory con trot standard:
Reference standaid True value (TV) Measured value (MV) %RS=MV/TVs 100 number (rag/L) (regL) (acceptable range - 90 to 110%)
66 o.lo0.50 a. "bq-/.
Reviewed by Date reviewed i)-K~ ~
Environmmental Testing Solutions, LLC Page q-:
Page _ of L.
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water,-MDL =0.10 mgL Meter: Accumet Model 25 pH/Ion Meter Iodide reagent:
Date analyzed to. 0.I Acid reagent:
Slope:
Calibration:
=e-... -Im/L Reference standard number I 0
LN55.* + I 1.00 MWL I &1 I i
Note: For samples with a residual chlorine of > 1.0 mngfJL the calibration range must be adjusted to bracket the chlorine levels ofthe samples.
Laboratory conirol standard:
Reference standard True value (TV) Measured value (MV) % RS = MV*I TV x 100 number (mgIL) (1ag/L) (acceptable range - 90 to 110%)
/ Dlc sm le o 0.50r Duplicatesample precision: uup cacernc Reiulhlrn %RD=(S-)I[+)1Jx10 ISamp~leA Q. 1. Mf ,..
number -- * .... ',*
- ample cnaracteristcs Residual chlorine (mg/L) % RPD = {(S -D)/[(S+D)/2]}
- 100 (acceptable range - L10%)
a0z40 i o o- tqcvu--.& Co-- OO _
Duplicate D 1-0.0 0 5uto2 Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number N- Blank (should be = < 0.10 mg/L) *i*/,, i 62 10 10.0-Z t..LJ0PE(
c) At; Note: All samples were analyzed m exces of EPA rýA, .l.... 1 -..... * ._,.
0S" , ! " -...- ,' - - 5 -- lU
- unl' uess otherwisC noted.
-Laboratorycontrol standard: C"1.WC~
Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mZfL) (mg/L) (acceptable range - 90 to 110%)
V~))10.50 11. 67 Reviewed by Date reviewed L Y .
Environmental Testing Solutions, LLC Page Page _of Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pH/Ion Meter Iodide reagent:
I-Date analyzed "- Acid reagent Slope:
Calibration:
Reference standard number I 1k)6 i0q I &=C ]
Nofe- For samples with a re3idual chlorine of > 1.0 rag/L, the calibration range ms be adjust to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV) Measured value (NM % RS = MV / TV x 100 number (mg/L) r(mg/L) (acceptable range - 90 to 110%)
) !!N0.50 b144 q.6&2 Duplicatesample precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) /[(S+D)12]} x 100 number (mg/L) (acceptable range- 4-10%)
Duplicate ,,D
- 00 t o2.
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mfL) 7....Blank (shouldbe=<010mgfL) ----"' - '-"-*---------'-.. '-.'-'-'
0201.0 an.
OZ I~or ()u. ___ ___ ___ ___ 0.2
______H_
Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
% RS=MVITV 100 cceptable range- 90 to 110%)
Zk,!. 1 Reviewed by Date reviewed
Sequoyah Nuclear Plant Biomonitoring October 08-15, 2002 Appendix D Reference Toxicant Test and Control Chart
hrnvironmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately. hard Synthetic Water Test number Test date 7-day IC25 CT S Control Limits SA.le Warning Limits SA.2s Control Limits Cv (g KCVL) (g/L KCI) CT-2S CT+2S CT-Sma CT+SAJI.
CT - S. Cr +SAM 12-12-01 057 2 01-04-02 063 0 60 004 052 069 0.07 048 072 0.13 0.39 081 3 02-05-02 049 056 007 007 042 0.71 0 07 044 068 0.12 4 02-13-02 061 0.35 0.77 0.13 058 0 06 045 0.70 007 046 0.70 0.12 0.37 0.79 0.11 5 02-26-02 0 65 059 006 046 0.72 0 07 047 071 0.12 0.38 080 6 03-28-02 056 0.11 0.59 0.06 0.47 0.70 007 047 0.71 0.12 7 04-23-02 0.38 0.80 0.10 0.70 0 60 007 046 0.74 007 048 8 0.72 0.13 0.39 0.81 0.12 05-07-02 061 060 0 06 047 0.73 0 07 0.48 0.72 0.13 0.39 081 9 05-23-02 0.76 0 62 0.11 008 046 0.78 0 07 050 0.74 10 05-24-02 0.13 0.41 0.83 0.13 0 66 0 63 008 047 0.78 0.08 051 0.75 0.13 D.42 0.84 0.12 II 06-04-02 0.67 0.63 007 0.48 0.78 008 0.51 0.75 0.13 0.42 084 12 06-11-02 0.70 064 007 0.12 049 0.78 008 052 0.76 0.13 0.43 13 08-06-02 055 063 0.85 0.12 0.07 048 078 008 051 0.75 0.13 14 08-13-02 0.58 0.42 0.84 0.12 0 63 007 0.48 0.77 008 051 Is 0.75 0.13 0.42 0.84 0.12 08-20-02 0 63 0 63 007 049 0.76 008 0.51 0.75 0.13 0.42 0.84 16 09-10-02 061 0 62 0 07 0.11 049 0.76 007 0.50 0.74 0.13 17 09-17-02 064 0.41 0.83 0.11 063 0 07 0.50 0.76 008 0.51 18 0.75 0.13 0.42 0.84 0.10 10-01-02 060 0 62 0.06 050 0.75 007 0.50 0.74 0.13 0.41 0.83 19 10-01-02 0.47 062 0 07 0.10 047 0.76 0.07 050 0.74 0.13 0.41 20 10-08-02 053 0.83 0.12 061 0 07 047 0.76 007 0.49 0.73 0.13 0.40 0.82 0.12 Note: 7-d ICzs = 7-day 25% inhibition concentration. An estimation of the concentration ofpotassium chloride that would cause a 25% reduction in Pimephales growth for ie test population.
CT = Central tendency (mean IC25).
S = Standard deviation of the IC25 values, SA&a = Standard deviation corresponding to the 11percentile CV. SAjo = 0.12, 10 as determined by USEPA for the method and endpoint.
SA.2s = Standard deviation corresponding to the 254i percentile CV. SA.S = 0 21, as determined by the USEPA for the method and endpoint.
CV = Coefficient of variation of the IC25 values.
Control and warning limits were established using the standard deviation of the IC2s values conreponding to the 10 'hand 25? percentile CVs These ranges are more stringent than the control and wusOS limits recomunended by USEPA for die test method and endpoint.
USrPA Recommended Control andWanming 1-nina Warning Limit - Standard deviation corr*spondsng to the 75* percentile CV SAM - 038 Control Limnit - Standard dcviaiion corrfspondiiig o the e percenlile 90 CV. S,. -0 45 USEPA. 2000 Understanding and Accounting for Method Vanability in Whole Effluent Toxicity Apptications Under the National Pollutant Discharge Elimination Progrna. EPA-833-R-00-003. US Environmental Protectlon Agency. Cincinnati, OIl Organismsobtainedfrom Aquatic BioSystems. Inc 0.-08-02.xh
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Piniephalespromelas using Moderately Hard Synthetic Water 1.2 1.1 1.0 S0.9 Q
0.8
' " 0.7
= 0.6
',,- 0.5 0.4 0.3
.<? . ~ . , . 4. .i Test date 7-day IC 2s = 25% inhibition concentration. An estimation ofthe concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
-- - Central Tendency (mean IC 25)
....... Control Limits (mean IC25 +/- 2 Standard Deviations)
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 1.2 W C i S i t 1 a 2 P il C Vs.
1.1 --
Warning and ControlLinmits Set According to 1 0 *gand 2 5 dI Percentile C Vs.
1.0 S0.9 0.8 * *"......... ........... .............
S0.7
- 0.6 i-- 0.5 0.4 1- . ...........
° ....... ........... ............ .............. , .. .o . . . . . . .. . . . . . . .
" °o'*.°...°... .. . . . . . . . . . . .
0.3 [-
allI a,. a a i i Test date 7-day IC2s= 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Phnephalesgrowth for the test population.
Central Tendency (mean IC25 )
Warning Limits (mean IC25 A: SA. 1o SA.0 = 0.12, as determined by USEPA for the method and endpoint)
......... Control Limits (mean IC25 L S A25s,S-25 = 0.21,-as determined by-USEPA-for the method and endpoint)
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromelas using Moderately Hard Synthetic Water Test number Test date Control Control Mean Survivwl Cv Cr MSD PMSD Cr Growth 0%) (mgnlarvae) for Control
(%) for PMSD (D)
Growth CV (o)
I 12-12-01 95 0.824 16.6 0.19 23.5 2 01-04-02 95 0.799 10.1 13.3 0.18 22.0 22.8 3 02-05-02 97.5 0.948 6.9 11.2 0.21 22.2 22.6 4 02-13-02 100 0.648 8.0 10.4 0.10 15.5 20.8 5 02-26-02 100 0.642 3.3 9.0 0.11 16.5 20.0 6 03-28&02 100 0.629 11.2 9.3 0.13 21.4 20.2 7 04-23-02 100 0.712 12.8 9.8 0.19 26.2 21.1 8 05-07-02 97.5 0.458 12.3 10.1 0.07 15.4 20.4 9 05-23-02 100 0.378 9.9 10.1 0.09 22.5 20.6 10 05-24-02 97.5 0.444 21.3 11.2 0.11 25.0 21.0 11 06-04-02 100 0.682 14.0 11.5 0.15 22.1 21.1 12 06-11-02 100 "0.650 3.7 10.8 0.11 16.6 20.8 13 08-06-02 97.5 0 694 22.2 11.7 0.16 23.5 21.0 14 08-13-02 100 0.659 13.6 11.8 0.12 17.5 20.7 15 08-20-02 100 0.765 8.5 11.6 0.12 16.3 20.4 16 09-10-02 100 0.854 1.2 11.0 0.11 12.5 19.9 17 09-17-02 100 0 824 13.4 11.1 0.12 14.6 19.6 18 10-01-02 97.5 0.750 18.4 11.5 0.19 25.4 19.9 19 10-01-02 100 0.975 12.7 11.6 0.13 12.8 19.6 20 10-08-02 97.5 0.929 8.0 11.4 0.18 19.5 19.6 Note. Average control growth (mg/larvae) - 0.713 CV = Coefficient of variation for control survival.
"Onaverage, the CV for control growth is 11.4% in Environmental Testing Solutions, LLC Punephales chrarnc toxicity tests Lower CV bound determined by USEPA (10' percentile) = 3.5%.
Upper CV bound determined by USEPA (90"' percentile) = 20%
MSD= Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.6% from the control Lower PMSD bound determined by USEPA (10* percentile) = 9 4%
Upper PMSD bound determined by USEPA (90'A percentile) - 35%/,.
CT = Central Tendancy (mean Control Growth CV or mean PMSD)
The lower and upper bounds were calculated by te USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests USEPA. 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Organums obtainedfrom Aquanc BioSysrems. Inc L0-08-02.xis
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Piniephalespromelas using Moderately Hard Synthetic Water I I I I I I I I I - I I Ii I I I I I 40 F 30 k S20 10 [
fi i i I i I, ,I I I I I t I Ii I I 0
'9Z' '9Z '9' - R
- 4 '&011."I Test date PPMSD = percent minimum sigmficant difference. PMSD is the minimum significant difference bctween the control and treatment that can be declared statistically significant.
-Central S-Tendency (mean PMSD)
... Lower and Upper PMSD Boumds
- 35%
Lov%,r PMSD B3oaud (1O' percentile) = 9.4%, Upper PMSD Bound (90'h percentile)
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint)
,Environmental Testing Solutions. v,."
Iarp A Uk .J Potassium Chloride Chronic Reference Toxicant Test (kPA/600/4-91/002 Method 1000.0)
Species: Pimephalespromelas PpKCICR Teat Number.
Dilution preparat KCI CHM numberi.
Stock preparation: 50 g KClIL: Duo Deimiz water Diluton prep (mgiL) 300 450 600 750 900 Stock volume (mL) 6 9 12 15 18 Dilucnt volume (mL).I Test oranis inormation: X. . Test information:
Organism age: L-7Z g5 o 0 L r) Randomizin template: EL)
Date and times organisms I ' H-
-M j1 Incubator number:
wereborn between: N-o*i-' s h "t Organism source: AT -O"-02.0 Artemia lot number:
Transfer bowl information: Cf pH Temperature = Oven temperature: o z'C.
-__ __ __2",i'L Dryingtime: tc-i1d,*j.
Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 0 ,o-o-oo /o .. Oa I 10--02o /0 2'oO 7 16 IControl
% Mortality 7 to - IS.
infornmaton:
bI I2", 72o%
- ta Summaryofltestendpoints:
7-day6LC0O ______9
- exage wCz19tL per arvae: 0.9Z9
, >-. 0.25 m*arv-,m NOEC 4../c S*0.25 Aarvae NOEC LOEC -- 4,00 ChV Sig. (0 IC25 925.1 1C25
PPKCIC~R Test Number~ 3
- 1 Survival and Growth Date L Day CONTROL I____ 300 ing KCI/L 450 Me KCIIL A
A F G 0 IE H
/0 10 10 10 IC)
.1c /0 /0 2 /0 A)~ /J3 /0/ /o l0 10 3 10 IL) L) / ~0 ito 10 IL) 10 10 /o 10 10 10 10
/0 10 /o /
6 6 /0, '"
f0 10 L9 1(3lc 10
-7 1o II A
aneigt(mg) 9 - .11p-I IP I
b I /0
- .1 I I) IC 1610 B - Pan +Larvae weigM (rmg)
ILL A,!
1')
Larvae wei2Jit (mg) -A - B IL , - I#jk . 1b - 1-Calculations and data reviewedL Comnments:
X-age j 01
'PpKaCPcRTst Number. 5d' 12 Calculations and data reviewed.
Commnents:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/60014-91/002, Method 1000.0)
Species: Pirnephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: PpKCIL'R B 36 Rte vd w t~d b Test dates, October 8-15, 2002 ketikais IsieW a eberof thai aSeobo,08 A - ri. xng.sts'fJ L.nu wdgIE tJ
-A-5 JI Wdei.tl',SI l.kIMem{)- ties. s.n$..l
(%)
1 Mess wdg*dt.aJ 1 ssst tl 2-1 CresirsiM. (mi. RgpUcst¢ i.".d.u-drro F0 .. r. A - Ila. dithi OWg Wrigtt (m V ¢mnl (%l SC*t) WrIvii¢ 9 14867 24230 9363 09363 A 10 25050 10038 10038 97.5 0.92111 Le Not appliable B 10 10 15012 Contrul 23220 84964 8256 08256 1
10 10 15086 24 580 9494 09494 10 149933 23960 9027 09027 E 10 1000 0.024 13.A F 10 10 14984 23750 8766 08766 1 10 10 15161 20430 5269 05269 N G 15005 24040 9035 09035 U1 10 10 10 10 14807 23 100 8293 08293 1
01913 97.5 0.295 5.3 11.7 10 10 15257 23 170 7913 450J 23620 8931 08931 K 10 10 14689 08043 ,,,
9 14777 22820 8043 L 10 7 14739 20700 5961 05961 10o 10 6 15 131 21300 6169 06169 700 0.5793 6.0 37A N
600 10 7 14991 20370 5379 05379 14646 20,310 5664 05664 P 10 8 2 IS019 17070 2031 02031 Q 10 03523 25.0 0117" 44.5 7" R 10 4 14,797 18 320 3.523 750 S 10 3 15185 17,130 1945 0 1945 15 149 16360 1211 01211 T 10 3 14"51 37280 2529 02529 U 10 01680 SO 0.1134 104.2 87.1 V 10 2 15 160 16840 1680 900 15054 J5,380 0326 00326 IVW 10 1 0000 0000 00000 X 10 0 0000 MSD " Mmnnumn Significant Difference Dunnelt's huSD value: 01814 195 PMSD - Percent Minimum Significant Difference PhISD: and liatitint dta c be control PMSD is a measure of test precision The PMSD Is the mntimumIperesil difference berwem the ocns fr Envirwsinrutalr T"ing de.lared statistically significant in a whole effluent toxicity test On average. &significant differtace the contol (determined through Solutions, LLC chronic toxicity tests when a toxicant reduces Putiephales growth by 19 6% from reference toxicant testing)
Lower PMSD bound determined by USEPA (10th perceistile) - 9 4/..
Upper PMSD bound detennmined by USEPA (90th percentile) - 35%.
Labot'toie for Pinim o id piwth ral The lower and upper bounds were calculated by the USrPA using 205 tests conducted from 19 chronic reference toxicant tests Under the National Pollutant Discharge Ehlmmatton Program PPA-833-R-00-003. US EAvlrbeematal Pretectica USEPA 2000 ulndcrmtanding and Accounting for Method Vancibilty ntWhole Effluent Toxicity Applications Asentmy, Cincinnati, Oil I0-08-02 Ia Organistm1 obtainedfrom Aquatic BwS)Irtems. hic
Environmental Testing Solutions, LLC Statistical Analyses LAAV!rub cnwa taw Sntva j;;77 DSyGiWý lad Dm~ IC2SI= LkbUk Ur3.. TO" Sdjub" &t~.9"n XCLPctuama dilmid D.Coaeel I'9m6 LOS13 U.1 0.9494 3009 01027 0766 0.526 0.9I 430 a.im9 0.7913 0.195I 1042 600 0.596 0.6169 0.579 0-5664 730 0.202 0.3M 0.19"s 0.1211 900 0.252 0.1690 0.026 (LOO TM Tmaw.*ý Uwa-fiarwd Cmo.-V. .m N-Mmoa mana I-Talld Iiatkal P.4 MaE CV%/ N "Wta OjCoon. 0.9281 WCO Cr6A&W MID0 hit NA O.921 0.1256 L0039 &.047 Coo 4 0.104 0.3640 0.104 0.5269 0.92n0 2.051 0.9035 22.9644 4 U.19 450 0.129 0.2931 1.100 01114 a81260 0.171 0.929 0.7913 0.W191 3454 600 4 1.193 2.110 0.1914 0.5793 0.6Z32 0.5793 0.5379 0.11L60 0.M1 0.6169 5960 4 730 0.2172 0.2544 0.2171 0.1221 0.379 0.6238 0.3523 44-525 4 900 0.1134 0.1221 0.1134 000 0.2178 0.234 0.2529 104151 4 0.1234 0.1221 AaijMayTemt Shp WW Stdakc Crltm tamdaa aunn&L dumbwen (p 0 01) Sk- Kurt Bmet1ts Tamwa0acato 003457917 0.805 equ2eaýa (p -0002 -17150174815-5373924115 Hypohlu.ITrt 11-ftaL. 3025M&0171 921035099 GL09! 190C LOEC Cv TU PASN PMSIp DauranowsTa MEB ME F-Prob df 430 >420 0.1213629 0 195271m0 002 7701936 0013942479 0324476004 2.1 Polul Xa/L So 5' CL4Exp) Skm~
1C02* 122549 2113. 3303 30393 10147 2C10* 24699 127.52 6606 60193 0.1911 ICIS 46610 10605 41.29 31620 .10953 IC0 496.24 7242 22076 SU.447 .1M20 IC25 32567 4002 43331 57347 -420160.
IC40 6091 211! 5"616 631.24 412200 02 92064760 20 33 62990 60922 0652907 wdruzut IC=twmae lind=o a*.Ioweoiaox nnoo a 06.
c 05 04.
0.3 0.2.
01 0.0 .. . . .. .
0 200 400 B00O 800 1000 Dose mg/L 1.2 S08 0 05 leve!
1.....................................-tail.
of sigrullcance o06
'~04 0.2 Org~anunu obtained from .4oyatc BtSitoyewm. Ina~
xIs 10-409-02
b' or° PP:KC1CP. Test Number.. 3 MIIS Control Chemistry:
Acceptance Criteriafor Daily Chemistry:
Acceptance Criteria Concentration (dilution must be rmade if the conducvty fals outside the CONTROL 280-360 300 mg KCI/L 840 -890 450 mg KCI/L 1100- 1180 600 mg KCUfL 1390-1460 750 mg KCIJL 1660 -1710 900 mg KCI/L 1890-1950 KCI Stock 67000 - 76000 (25 g KCI/L)
ot PpKCICRTest Number.
PpKClcR. Test Number: -340 (00-100 (o~OO(ý:73cc
( -790
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water Test number Test date 7-d IC2, er S Control Limits S.JO Warning Limits sAuS Control Limits (g/L NaCi) (g/L NaCI) CT-2S CT+2S Cv CT - SA*I CI + SjL, CT - SAm CT + Sx.
1 03-07-01 1 04 2 04-04-01 1.02 1 03 001 101 1 06 008 3 05-09-01 0 95 1.11 018 0.86 1.10 105 004 0.97 1.20 001 1.14 008 097 1.13 4 06-06-01 1 07 106 0.18 0.88 1.22 004 003 0 99 1.13 008 098 5 07-10-01 I 05 114 0.18 089 1.23 106 003 I 00 1.12 003 6 08-08-01 008 098
- 1.14 0.18 0 89 105 003 0 99 1.23 003 I 02 1.11 008 097 1.13 7 09-12-01 I 02 1 05 0.18 088 1.22 003 003 0 98 1.11 008 8 10-10-01 105 0 97 1.13 0.18 088 I 03 0 03 0 99 1.22 003 1.10 008 097 1.13 9 11-01-01 I 00 1 04 0.18 088 1.22 003 003 0 98 1.10 008 10 12-05-01 0 96 1.12 018 087 1 05 104 003 0 98 1.21 003 1.10 0 08 096 1.12 11 01-09-02 091 1 03 018 087 1.21 003 005 0 93 113 008 018 12 02-05-02 0 95 1.11 086 I 07 1 03 0 05 0 94 1.20 005 1.13 008 0.95 1.11 13 03-19-02 I 03 103 005 0.18 086 1.20 005
-094 1 12 008 095 14 04-09-02 I 03 1.11 018 086 1.20 1.03 004 0.94 1.12 008 004 Is 05-07-02 0 95 1.11 0.18 086 I 05 103 0 04 0 95 1.20 0.04 16 1.12 008 0 95 1.11 06-04-02 I 06 I 04 0.18 086 1.20 004 004 0 95 112 008 0 96 17 07-09-02 1.03 1.12 0.18 087 1.21 I 04 004 0 95 1.12 004 18 08-06-02 008 096 1.12 0.18 1.05 1 04 004 096 087 1.21 004 1.12 008 0 96 1.12 19 09-04-02 1 05 1 04 0.18 087 1.21 004 004 096 Ili 1.II 008 0 96 20 10-08-02 1 03 1.12 0.18 087 1.21 I 04 004 096 0 08 0 96 1.12 0.18 004 0 87 1.21 004 Note: 7-d ICIS = 7-day 25% inhibition concentration An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population CT = Central tendency (mean IC2,)
S = Standard deviation of the IC,, values.
SA.Lo= Standard deviation corresponding to the I 0 "hpercentile CV. SA t 0 = 0 08, as determined by USEPA SkAs = Standard deviation corresponding to the 25d' percentile for the method and endpoinL CV SA 21 0 17, as determined by the USEPA for the method and endpoint.
CV = Coetlicient of variation of the IC2s values.
Control and warning limtis were established using tie standard deviaion orthe IC2s values corresponding to the I0" and 25 "percentile CVs These ranges are more stringent than hconrol ad warning limits by USEPA for die test miethiod and endpoint recommended tJSt PA Recominended Control and WMlini I n-ifis_
Warning I.imil - Standard deviation corresponding to the 75"'
percentile CV. SA7,= 0 45 Control Liini - Standard deviation cormnponding to tie 90'h pen.htite CV. SA4o - 0 62 USEiPA. 2000 Undertandiig and Aucouniing for Method Variability inWhole Effluent foxicity Applications Under the Natiot;J Pollutant Discharge Eliminanon Program. EPA-833.R.00-0C3. US Eavironmoital Agency, Cincinnati, Oil Protection 1-0--02.xfr
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1 .5 1.4 Control Limits Set According to +2 Standard Deviations.
1.3
- .2
,,~... .. ° ....... ,.°..,.... ,
. . . ................... . .............. .,. . o .... oo ..... ,.
- o. -. ~ . .......
0.9 0.8 I I II , , . t i .I ! I I f_ I I I .
Test date 7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for die test population.
S- -Central Tendency (mean IC 2 )
. ....... Control Limits (mean IC25 + 2 Standard Deviations)
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1.5 i 1.4 Warning and ControlLimits Set According to'1 O"ý and 2 5ih PercentileCVs.
1.3 US.
M .2 ....................... .......... ................. :.................................... ....................
S..
- 1.0 0.9 .. . .. ............. ....... .. .
0.............................o...............................
0.8 Test date 7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Cerioclaphniareproduction for the test population.
Central Tendency (mean IC.2 )
Warning Limits (metan IC 25 - SA 10' SA 1o= 0.08, as determined by USEPA for the method and endpoint)
....... Control Limits . (mean IC25 +/- SA 25, SA.25 = 0.17, as determined by USEPA for the method and endpoint)
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphniadubia using Moderately Hard Synthetic Water Test Test date Control Control Mean number Survival Reproduction CV CT MSD PMSD C" for Control
(*/-) (offspring/female) (N) Reproduction (0h) for PMSD (%)
CV (%)
1 03-07-01 100 24.0 8.3 2.4 10.1 2 04-04-01 100 25.0 10.0 9.2 2.9 11.8 10.9 3 05-09-01 100 27.9 8.9 9.1 3.1 11.0 11.0 4 06-06-01 100 27.8 5.8 8.2 3.5 12.5 11.3 5 07-10-01 100 25.0 9.4 8.5 2.8 11.2 11.3 6 o8-68-01 I00 30.9 2.4 7.5 2.7 8.7 10.9 7 09-12-01 100 28.6 6.6 7.3 2.7 9.4 10.7 8 10-10-01 100 24.9 8.8 7.5 2.5 9.9 10.6 9 11-01-01 100 27.0 6.5 7.4 3.1 11.6 10.7 10 12-05-01 100 24 8 9.1 7.6 4.8 19.5 11.6 11 01-09-02 100 27.5 7.5 7.6 4.5 163 12.0 12 02-05-02 100 23.1 10.1 7.8 2.6 11.1 11.9 13 03-19-02 100 26.3 8.6 7.8 3.0 11.2 11.9 14 04-09-02 100 26.2 12.3 8.2 2.8 10.7 11.8 15 05-07-02 100 27.3 8.1 8.2 2.3 8.4 11.6 16 06-04-02 100 26.0 9.9 8.3 3.8 14.7 11.8 17 07-09-02 100 29.5 8.0 8.3 3.5 11.7 11.8 18 08-06-02 100 28.4 10.4 8.4 2.7 9.5 11.6 19 09-04-02 100 31.4 6.7 8.3 3.0 9.5 11.5 20 10-08-02 100 31.1 9.2 9.4 11.4 8.3 2.9 Note Average control reproduction (offsprng per female) = 27.1 CV = Coefficient of variation for control reproduction.
On average, the CV for control reproduction is 8.3% in Environmental Testing Solutions, LLC Lower CV bound determined by US EPA (10b percentile)
= 8.9%.
Upper CV bound determuned by US EPA (90'b percentile)
= 42%
MSD = Minimum Significant Difference PMSD = Percent Mmunum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 11.4% from the control.
Lower PMSD bound determined by USEPA (10* percentlle)
= 111%.
Upper PMSD bound determined by USEPA (90h percentile)
= 37%.
CT = Central Tendancy (mean Control Reproduction CV or mean PMSD)
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under National Pollutant Discharge Elimination Program. EPA-833-R-00-003. the US Environmental Protection Agency, Cincinnati, OH.
IO-08-02.rls
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 50 I I I I I I I I I q I I I I I 45 [-
40 F 35 F-S30 S25 1 20 15 10 5
I I I I I I I I I I I I I I I I U
, Ot 0ý 0. 0% S, "q 1<1 OzT date Test date PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- - Central Tendency (0mean PMSD)
Lower and Upper PMSD Bounds Lower PMSD Bound (10"' percentile) 11%, Upper PMSD Bound (901h percentile) = 37%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint)
Environmental Testing Solutions, i.L, Sodium Chloride thronic Reference Tozicant Test (EPA/60014-911002 Method 1002.0)
Species: Ceriodaphniadubia Date Time Analyst CdNaC1CR Test Number. 2 Tests=art J.08.-
0 I 1l, I it I Testend -IS -0,& ta7 I Li Dilution preparationinformation: Comments:
NaCl CHM number. o _ _ __ao_
Stock prepa-ation: 100 g Ns- (diuole 50 gNai i- 500 ml Ddution.prep (rag/L) 600 800 1000 1200 1400 Stockvolume (mL) 9 12 15 18 21 DhIut volumc (mL) 1491 1488 1445 1482 1479 Test organism information: Test information:
Organism age: IL Z4 11o L s Ale Randomizing te late: ELOP-Date and times organisms 0o-ol-o0. IsLl -To jkoq Incubator number:
were born between: 1 _ _ _ _ _
Organismsource: 10-01-6-Z A.& YCTbatch: 46*t5 /O.o).02 Transfer bowl information: pH .CfI Temperature (C)= 25.O Selenastim batch: 6.5 .jLo.OZ CONTROL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 I
!_Adult Young produced mortality 0 C.*) C-
- LL r - C : .
2 Young produced C) 0( 0C Adult mortality I-4 1 Young produced C C) 0_O _
Ad ult mortality \- ___t T5t Young produced I I 1_ L 1_ 1 1 1-
__ _ 12- 17 Adult mortality -- I -- ý- I_-__II_
6 Young producedI Adult mortalit Ii- t- - I L- I L I 7 Young produced 0 ) 0) l' 0 1 1Q 10 Total young produced Z7 7 ' 30 3C/ 31 50-3 .3 -L3-37 L- s I I L.- I .-- I L..- L.
Final Adult Mortality X for 3* Broods '><) I I..5 -- I><
Calculations and data reviewed: .4f
%Jr Test Renewal, Feeding, and Randomizaton Record Label Mon Tue Wed Thu Fri Sat Sun Date kAL Of lQC
"(~ l -0J IGLL ( J2.
Time _LL& A20 Inco iV3 Shelf Location Control information: A.pta= ctera Summary of test endpoints (mg N**aCL):
% of Male Adults: M67. 20% 7-day LC50 >Moo
% Adults having 3' Broods- 1" zs80% NOEC_ _"_
%Mortality: :520% LOEC /00 Mean Offspring/Female: 1 _15.0 ofpring/female ChV ,gq. q
% CV: <40.0% IC25 I f02(p.9
Environmental Testing Solutions. LLC Paee 2 o! "I CdNaCICRL Test Number~ ,
600 mg NaCI/L Survival andReproduction Data
- - - tteumber Day_ 1 2 3 4 5 6 7 a 9 10 1 Young produced 00C) r 0 0 Adultmortalfty L L_ L- L _ _ L L.
2 2 Young produced Adl moIrtality 0 C. o~~F
- C3 -_ ... .
3 Young produced I C. n L 0 Adul mortaty L L L Lt t_ L L L t_-
5 Young produced 17-- !" i q IqI--
-1?- -)
Adultmortality o_ L %. L- I . LI L. Lu 6oa Young produced -7E 1-"q 11 Adult mortality L... L- L-L..- t JT.
- .I -- a3 = 12 -
Final Adult Mortality onpone '~ - ~.-3 3 - ,oalogroue
___ t.. 3 -
-L-I-2 :I.IIIII3 I L -'~
i0 3 3.III Concentration:
% Mortality: 0.,
Mean Offspring/Female: 30.9
%Reduction from Control: 0.67.
Calculations and data reviewed:
800 mg NaCI/L Survival andReproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Youngproduce 0 0D C: C) c) c) 0 0 0
!Adult mortality LL - I (._
2 Young produced __ 0 0
___ mortalityL
- p**d'Adult L L _ L L _
3 4 Young produced ~f I's 'IT 5 7Y o )l I I c L_.grdcdFJ mortali iAdult 6 Youmg produced T C C 1j0 Adult motlt
___ Youngprodued 6t Youngproced ,16 - 0 J L gO
,0 9 L-izI** _
L 0.
L -. I z
10,Y 2-1 1.
L. -
92 0~5 Final Adult Mortality j - L. --- - U I L - T Ito.zt.-o2.
Concentration:
%Mortality: 0%
Mean Offspring/Female: ??.(o
% Reduction from Control: "o" Calculations and data reviewed:
Environmental Testing Solutions, LLC Page 3 ot 7
-- Page 3 CdNaC1CR Test Number. A:
1000 m NaCl/L V 1 2 1 3 4
- ~ct 5
ummber__
6 7 9 9 10
, Young produced 0 Q 1 0 0 0 o
- .,,,t,,
-Adul mortality r L,*,
t_. t... _. L_ _. L .. L L L 2 ,
Young Ye.ag peoduced podnced- g!_ 0L- L.. ._ . ._. .-..3 \
CL.... -(
I Adult mortality *- -- L- L- L. L L A~h= tt-
.- L- .
- -I -
4 Youg produced c0 1.0c00 0 0 Adult mortaitMy 5 Young produced - - - -
z 6 3 Youngaproduced Young 1 1ri-2-1 poducedeqtr3ationL an1 od o Da Adult mortality 7 Young produced 0- o 1 0 0) 0 0 0 Total young produced Z-7 Ij 2(. Z4 27 30 FtnalAdult Mortalty mortaalitywed . T.
C T. - - . .. L_
Calduaion .
Concentration:
%~~0 Motliy IMean Offspring[ffemate: IZ,
%Reductionfrom Control#:,(ý qV Calculations and data reviewed:
1200 mg NaCIJ Survival and Reproduction Data
____Replicate number____ ___ ____ ___
Young produced 1 021 c17 4 L 9110 Adl motait L.. ffi 2 Young produced ~ ~1C) # S9----TQ1-Q
_Adul tmor talty1R i1 ~ 7 ILI__
4 IAdult Young produced 1LEEE mortality j T - j
,3 1:3101 U _ _ _
o 01_
o
_ U __
61 Young produced __17 1-5i-HLq 1_
0_-7 1
6 prdue pz LZ (f I C_,
L/tm I FnlAdult mortality J-t - L 1L7 =LNIH L,
(
Environmental Testing Sointinnc T .T.
Page 4 ot 7 CdNaCICR Test Number. A 1400 mg NaCI/L Survival andReproductionData 1 Replct number 2 3 4 5 7 _T 8 9 10 1 Young produced 1 6 __m 0
I i 0j 0 0(5 0-ý 0 2 Young producedL Adult mort lt . - I L2 0 . _
3 4
Young produced
- Adult mortality Young produced
'*
- C) I*
- tI)]
t l Adult mortality U. . .
young Y produced 0 C0 61 Young produced1 Adult mortality I yugpouced tl Mortality Final Adult 0 Final Adult Mortality 0 0 i - -S. .:. j-
- b-Concentration:
%Mortality: -r I26 Mean Offspring/Female: '*.
% Reduction from Control: '12.'j Calculations and data reviewed:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Auialyses Test number: tdNaCICR #27 Test dates: October 9- IS, 2002 Revelwed by: 1&.. f HI Concentration Replicate number Survival Average reproduct~on of P ot redus film (mg/L NaCI) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) varadona CA) c-"e (CA)
Control 27 28 30 34 31 30 31 31 32 37 100 31.1 9.2 Not applicable 600 28 33 29 31 30 31 32 32 30 33 100 30.9 5.4 0.6 800 32 28 30 35 30 31 30 27 31 25 100 29.9 9.3 3.9 1000 25 26 27 30 19 26 26 27 24 30 100 26.0 12.0 16.4 1200 3 4 7 12 6 9 12 1 3 4 100 6.1 63.0 80.4 1400 6 0 0 0 4 5 4 0 0 3 80 2.2 110.9 92.9 Dunnett's MSD value: 2.923 MSD - Minimum Significant Difference PMSD: 9.4 PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 11.4% from the control Lower PMSD bound determined by USEPA (10'h percentile) = 11%.
Upper PMSD bound determined by USEPA (90"b percentile) - 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cernodaph/ia reproduction in chronic reference toxicant tests.
USEPA 2000. Understanding and Accounting for Method Variability in Whole EfIluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, Oil t.
Environmental Testing Solutions, LLC Statistical Analyses Start Dam IIAM~0 EdD.ta Tat1D Cd4.CCR M2IS= Lab M - Sample ID-S-EM. Testing Soheo RE-ae otisxr Sazmple DU Sampl. Type- NACL-Soms P rotocol EI AF 91-EPA Fr b .w ' -lor" Tet Spcie a du commeu C4o..,L 2 3 4 0.!munl S 7 27 000 23000 300 £ 0O 34 000 31.000 30.000 600 23.000 33000 3l.0 31000 32.000 29000 31000 30.000 37.=0 2OO 32.000 31.00 32.=0 32.000 21.00 30.000 35.000 30000 33.M0O 1000 30 COO 31 000 30.000 25D00 26 000 27.000 27.000 31.000 23.000 30.000 9 000 "26000 1200 3.000 26000 27.000 24.000 4400 7000 12.000 30.000 1400 6000 9.000 12.000 6 o00 0000 0000 1 000 3.000 4.000 0.000 4.000 5.000 4.000 0000 0000 3.000 TrausI'ee: Umu'trufornmi 0d-Tailed lset~tk Conc-g/1. Mean N-Mea Mean Ml Ma2 D-Commol 31100 CV% N l-Stat 1.0000 31.100 Crikl MSD Mean 600 37000 911 0 N-Metn 30.900 09936 30900 3100 21000 33000 5.383 10 0000 8g0 29900 09614 29900 0.56 2.217 2.923 25000 30.900 "1000 26 00 08360 26000 19000 35.000 30000 91254 10 0939 2.217 2.923 29900 0 9936 09614
- 1200 6100 12.027 10 3990 0 1961 6100 I000 12.000 2.287 2.923 26000 03360
- 1400 2.200 62.996 t0 19.557 0.0707 2.200 0000 2.287 2923 6100 6000 110927 10 0 1961 22.608 2.287 2.923 2.200 0.0707 Auxiliary Test, Kolnogorov D Test cates omal dsbo (p >001)
Bsneus Test i &niate 1 variances
..- tt Critical By~rtlett TestindicteseuWvwc. 030)
(p Skew Ka 0 ( 30 NIC OC 0735521972 E t!TU 6 050591469 2035 0.130465747 MSDu 15 08631706 0 157235946 Dunners Test MSDp MSI 800 MSE F-Prob 000 894427191 df 2.92306669 00939-923 9.346667 37037037 JE34 5.54 Point
- L SD Linear Interpolarou (80 Resample) 95% CL Skew Co , I8232052212 92
.. .7273535 33 5 373336269 1 -14549 ICI0 8979487179 5419916836 767.2633889 ICIS 9617269737 .08484 9776923077 3931064085 8757670773 100983257 -09661 1C20 1011.*56231 1576437826 953 7145833 10 ,
[C25 1024769496 -22154 1026834422 9011L51569 1006663923 1039 706422 -02877 IC40 0.9 1073763844 768929744 t0583848531 ICs0 2033.53333 -00D946 110502 5126 7757888513 1091412451 a08 1222229167 00342 07o 06.
04 03 0.2 01 00 0 500 1000 1500 Dose mojL.
Dose-Response Plot 40 35 30.......... ...............................................
o25 N-all, 0.05 level
" of significance
~20
- 15 10 T lO-.8-02.x-r
Lnvironmental T*estiug boiunons, LLU CdNaC1CR Test Number .
MHS Control Chemistry:
Parameter Control Batch:
(s.u.),
pH
- 1 n* 7*,
DO Conductivity A Alkalinity (mg CaCO3IL) ,. o Hardness (mg CaCO3 I.) F__. I_ _ I__-
Acceptance Criteriafor Daily Chemistry:
Acceptance Criteria Concentration (dilutioas must be remade if the conductivity falls outside the acptable range)
CONTROL 280 - 360 600 mg NaClIL 1390 - 1590 800 mg NaCI/L 1780 - 1970 1000 mg NaCIJL 2190 - 2370 1200 mg NaCI/L 2550 - 2740 1400 mg NaCIIL 2910 -3160 NaCI Stock 110500 - 116300
Environmental Testine Solutions. LLC P.~ A +, '7 CdNaCICR Test Number L.
Da1.o L3 3c~
zS. 2 GZo I00 qoO0 108;0
Environmental Testing Solutions, LLC Page 7 cd 7 Environmental Testing Solutions, LLC Page 7 at 7 CdNaClCR Test Number: -2:7 centration Parameter3456 PH(S.U.) .~ . 3.~.9 (a 4.
DO (gIL) 8.1. .- 4..9 NTROL Conductivity Temperature 2S-1 1 -ZL 7A 7A
-- iq.?~
PH (S.U.) e@.OL) 8.0 0.0osz c DO (mg,'L) .G.1 mg NaCU/L Conductivity Temperature
- Z.Z qq 7 Z- 2A., 24 DO (mg/L) 8. 6.0 8-0 9.0 .9 8.1 -. 6 4.
I nmg NaCL/L Conductivity I(4-1S 3, 93 (prnhos/m Z Temperature 2-4J q.9 .. Z4c.L -.
(0C (0c_____
pH (S.U.)0.
DO (mg/L) (3-1 130 6. o 6.0 L. 84.(
)Omg NaCIIL Conductivity 2q22Q (gmhos/cm) 22223421Z Temperature IZ4, f q 2q,
_____C(C) Z-.I -q 7 Mq.2q. 2qx.( z pH (S.U.) 6,4 6.(3q 8.0r e-03 e ZA 6.1 9.0-4 g..c DO (mg/L) e.2 8.0 (9. 8.k .~l e.
DOmig NaCIIL Conductivity (4
(Wnnhos/cm) z5t Temperature 24zq v.,2~ji~ Z~
pH (S.U.) e .o S, ,q e)o 8.o4 -qOwlA.3.i DO(m/L)3. Z 8.18.2 8e.c -4q If1 00 mg NaClfL Conductivity -q92 lz-T-1 (pinhos/cin)
Temperature 25 qz. zz?2.7 z.s 2,1. .- 0
( 0 c)I I_____ q z Z IL21 Z/.7E I Initial - 1 Final I Initial 11 Final I Initial 11~,Final I Initial I Final I 1luc (18(000 1036y)
PERMITTEE NAMEIADDRESS (Include FacilityNama/Locaton If Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 NNNNNN . TVA - SEQUOYAH NUCLEAR PLANT PTN026450 103HG NF - FINAL
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SOLIDS, TOTAL SUSPENDED SAMPLE 115 149 (26) *****13 17 (19) 0 5 /31 GRAB MEASUREMENT 0 iiiii!i.:::..* LBS/DY * ;**::::*:: 30 iiiiiiii**iiiiiiii! 100iii: :::i~ii~iiiiii~i MG/L :ii!:i:i::i:iW *ERKLY ii i::ii:
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SAMPLE MEASUREMENT SAMPLE MEASUREMENT EPAFor 320-(EV i99 PeviuMEdAioSURMabENuT Pg 1o SAMPLE MEASUREMENT M ..
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NAME/tITLE PRINCIPAL EXECUTIVE OFFICER ,Certify under penalty of law that this document and all attachments were prepared under my (,* -N .. TELEPHONE DATE dilrection or supervsion In accordance with a system designed to assure that qualified Richard T. Purcell personnel properly gather and evaluate the information submited Eased on my inquiry of the person or persons who manage the system, or those persons directly responsible for gatheringnvSprio42 83760 2 11 4 the information, the information submitted is, to the best of my knowledge and belief, true,42 83-60 2 11 4 Site Vice President accurate, and complete lam aware that there are significant penalties for submitting false A EO RN IA XCTV ......... L...................
__________________________ formation, Including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA' NUMBER YEAR MO DAY TYPED OR PRINTED ____D_,
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (reference all attachment( hRre EPA Form 3320-1 (REV 3199) Previous editions may be used Pagel1 of 1
PERMITTEE NAMEJADDRESS (Include FacillityNameAoccatfon IffDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)- MAJOR Form Approved DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No 2040-0004
.Name TVA - SEQUOYAH NUCLEAR PLANT
.&.ddPALI 7_X.............. 4TN0026450 1I 107 F-FINAL PERMIT umER D HMETAL CLEANING WASTE POND S. .
Loc~ation
_ A -_DQYAISYI_ ýA
.. -M AMILTOCOUNTY
- - -_- WONITQRING PFRIQD EFFLUENT From 1o2 IFrm 1!EAi10ololl To 1o2tlol31 Mo I DAY Y, I MCo I r)AV
...NO DISCHARGE EI .I.
ATTN Jerry D. Osborne NOTE' Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE **
MEASUREMENT (12) 00400 1 0 0 SU EFFLUENT GROSS VALUE . .. ... _______ I.....
____________ rMxUr.:...:........ . ..
PHOSPHORUS, TOTAL (AS P) SAMPLE **
MEASUREMENT (19) 00665 I 0P6RM I . . ... MGIL .lY C.MOS EFFLUENT GROSS VALUE *..._MR ...... . -.
SOLIDS, TOTAL SUSPENDED SAMPLE (19)
MEASUREMENT 00530 1 0 0:: EQ R
- N ii:i!i:PERM{'ITiiilii ///!iii:i*i* :iiil
- !:iii::iiii~*i~ii~!iii!~~~!!!!::~!:::::::.. * :ii~i*::i .. i~i~
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t:iiiii~A)LY*:iii EFFLUEN TGROSS VALUE R;.::***:,,,,,:,:,,,::,** **::OUIR::::***'::::::: '**:iiii~ ii~iiiiiiii~iiii! i!Iii~liiiiiiiii*iiMENT "*ii!*.. ii~ liii*ii~i"iii iiiii!!. ....... iii~'!iii~i'!!iii:' i***~~i'ii'iii~i':
OIL 00556AND GREASE 1 0 0 SAMPLE****************** . .......... MGIL tAtY G
~~~~~~
01042 0........ 1. MEASUREMENT { ::.ii:i ~~~~
iii:!~i:, .EM... .*** .::iii:::iil
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....... :iii:!*::i M(19)/ iii!iiiii! ::i:iD ' Y iiii"( I EFS V LU LUNTGR M i ..... ...... :............:.... ... .
oF LUIo GOS VA U EFFLUENT GROSS VALUE
- .:REQIREMENT!**:*i;;*:::***;;
M:::::::: " .*..... : : : .... :. *
- *..... : *. ......... iitiiii !* ii~
- CROPPE, TOTAL (AS CU) SAMPLE **** **** **** (19)*
MEASUREMENT .......
PATMAUREM TRAMN ENT"' .......
IRONATOETA (AS FMEASUREMENT (19) 0 0545 1 0 0:: .* M*, , .. . .EOR.::.::;
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EFFLU E N TUGIRO S SEVA LU E..... ... ..................
. ....... .I.Y .......M. .
EPA~~~~~~ ~~ ~ ~ ~
For ~ . .30-.(E.39...e..sed..n.my.eusd.ae. o "ilrectlon or supervsion in accordance with a system designed to assure that qualified Richard T. Purcell personnel properly gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for galtheringEnrme 42 83760 2 02 11 11 1 4
the informatIon, the information submitted is , to the best of my knowledge and belief, true. 423 8 3-76 0 Site Vice President accurate, and complete I am aware that there are sIgnificant penalties for submitting false "TNATURE OF PRINCIPAL EXECUTIVE OR PRINTED MYPED (19co
)
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No phosphate bearing chemIcals were employed No Discharge this Period EPA Form 3320-1 (REV 3/99) Previouseditions may be used Page I of 1
PERMITTEE NAME/ADDRESS (Include Facility NameLocacon If Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved
.igm__ "TVA- SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01)
Address. 3QŽ..PO
- -_ JIOIY TN0026450 t110 G tF- FINAL
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_- -* - APi T. -. -L . PERMIT NUMBERý DIICHARGE NUMEg RECYCLED COOLING WATER Location HAMILTON COUNTY MONflOpINC PFPioD EFFLUENT ATrN' Jerry D Osborne yFro M) nAY I I FAR mo IDAY ...NOTE: Read Instructions['*before ...
NO DISCHARGE compleling this form.
PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE
- MEASUREMENT (12)
.......... . ........ L ...............
EFFLUENT VALUE::.::.OU~i ROSGROSS E ~. ........*;:::::::*!::::::::::i:*::*
REM;i-N::i :::;:::::::::::k:.r:'
............ 2.
\.:.:.:.;..2 2 :.$.X ................
j j~~ _ __
OLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT
- i .! .! i ::::..
...... * : ::...a M IL :O I ... C OIL AND GREASE SAMPLE ************** ****
MEASUREMENT *19 010420 1 0 EFFLUENT GROSS VALUE 0 PRT
- Qx:lRxM.::
MEASUREMENT (19)
VLE.!.
EFFLUENT~~~~~~ GROS .:....... .....
EFFLUENT GROSS VALUE :: EGU'! IREMENTi;ii::!~!!::i!i !!!:!!_::_: ........ DAILY M.X ___ __::: _::_:__""::::'":":".........
IRON, TOTAL (AS FE) SAMPLE **** **** **** (19)*
MEASUREMENT"*(9 01045 1 0 0 i::ii:iPERM IT :A:ei:ii~:.:* ***i:iiii:i:'e*Aee: *: ... f 0: M:GIL MGL iiiiiil~~::iii:~ DAILY
- i~* C...OMPO......
OM=~ Siii**:W**#i"*:iiii; EFFLUEN GRS VALUE. ..........
___________.. .. ~ DAI.M ~ :~......................
PHOSPHORUS, TOTAL (AS P) SAMPLE ******************
MEASUREMENT "(19) 00665 1 0 0* ::.. . . .. 1 .............. ** .. 0. MGIL . I; YM.O .
GROSS.VALUE _ __ _ __ _ .i_ _ _ _ .i_ _
.EFFLUENT _ _ _ _
- R"Mt_
X . ....
EPA5 For IN3301 FLOW, CONDUIT(RE OR39 THRU Prviu edtin SAMPLE maybe sdPge1o tREATMENT PLANT MEASUREMENT (03) 50050 1 0 0 ::::i:i::ii:lE~ Tiii::!~:ii; JiiiREg..ORT.ir fi~i*:i:
EI:.O .RT'ii* ?vGD iiii *,;;* *:i~i.. lii iiii: ..****,;,iii~~iriii*:ii:i::ii'~!~
- i "CAL*C*DitJ I:hrectlon or supewsilOn in accotdance with a system designed to assure that qualified Richard T. Purcell ;personnel properly gather and evaluate the rnformation submItted Based on my Inquiry of the Sit VieePesientor persons who manage the system, or those persons directly responsible for gathering VInmental Supervisor 42 83760 2 11 4 SieViePrsdetaccurate, and complete I am aware that there are slgnificant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE RNTD'formation, O inE including the possibility of fine and impnsonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA I UBR YEAR MO DAY COMM ENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page I of 1
PERMITTEE NAMEIADDRESS (Include FacilityName/ocafon If Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR) OMB No 2040-0004 hme.._ TV._A--SEQUOYAH NUCLEAR PLANT (SUBR 01)
_AddLeiL ._QE.2. _ - --
__ (I EPFLq 1U -A . ._N .E.. .S.-.?. . F - FINAL PERMIT NUMBER DISCHARGE NUME BACKWASH
-= 12Y- -DAISY. - _N_214 -_.
.QJVt _ -L_..Q*H.6"_1LEARPJ*NT _ EFFLUENT Loca rmn.J-IALTOQ__N__CUa-Y MONITORING PERIOD WFAR# MO DAY I VFEAR MO' DAY From 02 10o 01 1To 102 10 131 *** NO DISCHARGE -- ,
ATrN" Jerry D. Osborne NOTE. Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
__________________EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE 0 (00) 0 1 / 31 VISUAL MEASUREMENT 01345 1 0 0 PERMiiii:i.I Ti~iii~i: i!!!::**
- ii i~ii!!i!i: * :ii:!~.* ;!i:ii.:ii* *!!iiii~i!iiiiii * **::::*i:ii*i: :iilRE 'p.'OJ !*iiTPASS =0 i;::iiii~iii:iiii:iE :ii::i Ii*StUAL ::::.
EFFLUENT GROSS VALUE ......... .... . .... . . __
SAMPLE PERMIT MEASUREMENT GROSS EFFLUEN MEASUREMENT..
R.E.........
SAMPLE MEASUREMENT SAMPLE MEASUREMENT
- i! i *! i* i *' :: i *! i:. .......... ........
SAMPLE 0~ ...... ...
MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT EPA~~~~~........... Form (REV 3----eiu-diin-myteusdPge1o lirection or supervision in accordance with a system designed to assure that qualified Richard T. Purcell personnel properly gather and evaluate the Information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gat~henng ( ,En Irornmerntal Supervisor42 63-30 2 11 4 the information, the information submitted is, to the best of my knowledge and belief, true, . 2 -60 0 1 1 Site Vice President accurate, and complete l am aware that there are significant penalties for submitting false SIGNATURE")-- OF PRINCIPAL EXECUTIVE TPDOPRDinformation, including the poSsibility of fine and Imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA INUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here Operations Is required once per shift to perform visual inspectbons.
EPA Form 3320-1 (REV 3199) Previouseditions may be used PagelI of 1
PERMITTEE NAME/ADDRESS (Include Facility Namea1ocaffon If Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved hLclie-_ TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
OMB No. 2040-0004 (SUBR 01)
.. dreL .__*X.2PQ
_ _ -_IS _
M------D- _ TN0026450 I1117 G F-FINAL PERMIT NUMBER DISCHARGE NUMaER BACKWASH Location _jDAMILTON COUNTy. MONITORING PFRIOD EFFLUENT From Fo 1.YFARImMO 02 , 0 AY 0 I 102 To YFA 1j Mj 0 I1 )AV131 1 NO DISCHARGE E l ***
ATrN. Jerry D. Osborne NOTE: Read Instructions before completing this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE * * * ** * * * * *** * * *
- MEASUREMENT 0 (00) 0 1 / 31 VISUAL 01345 1 0 0 S, ***A.:::****. 4 .... * . . ..
- ..::.I REPORT PASS-O EFFLUENT GROSS VALUE S. .I..MO FAILs1 r
... I ........ . . . .
I I M TOTAL SAMPLE MEASUREMENT V 1 I 'I I f I I MEASUREMENT I
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify undJer penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified Richard T. Purcell personnel p roperly gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Informaltion, the Information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, aind complete I am aware that there are significant penalties for submitting false information. including the possibility of fine and Imprisonment for knowing violations TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here Operations Is required once per shift to perform visual inspections.
Lieused Page 1 of 1 EPA Form EPA Form (REV 3199) 3320-1 (REV 3320-1 3/99) Previous editions may Previous editions may be used Pagel1 of 1
PERMITTEE NAME/ADDRESS (Include FacilityNane'LocationYfDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004
.4;rne_ 1VA - SEQUOYAH NUCLEAR PLANT
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Location .HAMILTQN COUNIy I I NA*tN(iT*IIN(' PFPD1C'f1 EFFLUENT AIrN Jerry D Osborne From 02 YFro 10 MO 01 DAY m ,
ITolo 211o 31 1 !3!1]
- NO DISCHARGE F'* ...
NOTE: Read Instructions before completing this form.
PARAMETER QUANTITYOR LOADING QUALITYOR CONCENTRATION NO. FREQUENCY SAMPLE
__EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE ******* (19) 9**
MEASUREMENT 00300 1 0 EFFLUENT GROSS VALUE 0 ::.:X.R1: :
- .*E i........::.
-... ... iii... iiiii i ........ X i::;>:..
- i i lGII .. ...
SOLIDS, TOTAL SUSPENDED SAMPLE *(***)**
MEASUREMENT 00530 1 0 0 RASI1I
~ ~ ~
EFFLUENT U ~::...***:'*!::::::::::.
~ ~ ~ ... ~',,,,.~
EFFLUENT GROSS VALUE XUM~ _____X_
SOLIDS, SETTLEABLE SAMPLE (25*****
MEASUREMENT 00545 1 0 0 P...I ..... M E F F LU E N U T~.... ...... .A.:ii:i:!i:i:i:ii*~:)i:i)ii*i!i!'
EFFLUENT GROSS VALUE ... UR~~T FLOW, IN CONDUIT OR THRU SAMPLE (03)**** , *******
TREATMENT PLANT MEASUREMENT (03) 50050 1 0 0 iiiiii!!iPEPJ*! : i:REPOR~ii' )RErj omR* I'l MGD ....... :..ii.i...:.. ; NI:*I. E$T*::/
EFFLUENT GROSS VALUE '-': ...'": ... ' ......... M"AV* o"*:":.".O: M................. ..... ." .. ..... _..
SAMPLE
!i:i:i:ii~**:i* ~ t i:::):i:iii.i MEASUREMENT SAMPLE MEASUREMENT
.UIR
. E:M.EN
. . .. ............... ~............ . .........
.* 1.
. FL!L BRM!*I.T!
EFUN SAMPLE .. VALUE NUMOSR MO' DA...
MEASUREMENT
'AV.. P*...:::i:.......
EPA~~~~....... Fom32.1(E....Prvosedtos.a.eusdPge1o NAME/TITLE PRINCIPAL EXECUTIVE OFFICER IlCertify under penalty of law that this document and all attachments were prepared under my ( (- N F TELEPHONE DATE Rihr . ucl direction or supervision in accordance with a system designed to assure that qualified Richrd T Purellpersonnel properly gather and evaluate the Information submitted Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng Ek
( Er*Iron~mental Supervisor 43 8373 2 1 the Information, the Information submitted is
- to the best of my knowledge and belief, true. ** .. j43 8373 2 t1 1 Site Vice President accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE
_______________________________iformation, including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA NUBR YARMJA TYPED OR PRINTEDM COMMENTS AND EXPL.ANATION OF ANY VIOLATIONS (Reference oil attachments here Durtng this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
EPA Form 3320-1 (REV 3/99) Previous editions may be used Pagel1 of 1