Text

Discharge Monitoring Report for June 2003
	ML032040289
Person / Time
Site:	Sequoyah
Issue date:	07/09/2003
From:	Howard S; Tennessee Valley Authority
To:	Hannah C; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation
References
	Download: ML032040289 (107)
	v • d • e

Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy. Tennessee 37384-2000 July 9, 2003 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6"' Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah

Dear Mr. Hannah:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JUNE 2003 Enclosed is the June 2003 Discharge Monitoring Report for Sequoyah Nuclear Plant Please contact me at (423) 843-6700 if you have any questions or comments.

Sincerely, jS#Aa4_ 0J.J Stephanie A. Howard Acting Environmental Supervisor Signatory Authority for Richard T. Purcell Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):

Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 I '

U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555

PERMITTEE NAMEIADDRESS (Inciude FaltVNmeocatln Dffent)

Name TVA - SEQUOYAH NUCLEAR PLANT dreQ OX o200

_S._I..Y S ODDY AISY_ TN 338..

fed_!._ VA-SEQUOYAH UCLEARA PLANT Locaton HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIINATION SYSTEM NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR01)

I TN0026450 101 F-FINAL PERMIT NUMBER L

l DISCHARGE NMBE DIFFUSER DISCHARGE IMOdQNITORING zERIOD )EFFLUENT IYEAR MO DAY IlYEARTIDAYO WfN nlof~uaolc Form Approved.

OMB No. 2040-0004 I-I Froml 03 l 06 l 01 I Tol 03 I 06 l 30 l NOTE: Read Instructions before con NAMEIITLE PRINCIPAL EXECUTIVE OFFICER I C wr pmy of law Mt s docwt d al tdnt wee prepred uider my A

l TELEPHONE DATE decIon orsiper lsan in aondmnce vIth a ytmdndsdt atars at querdled penre U_

Richard T. Purcell r

elamer and ealuate go lormtion sibmitted. Based on my nquky of Mu Person ot ps

~~~~who mn Ow system,. or thoe pee dcl Mrnpoi h gsthwft ft Acting Environmental Supervisor Wcrittalm, the knnatlon ubmited is. to Mu best d my knowledge and beflef, tm, 9P 423 843-6700 03 07 14 Site Vice President aomxsts. and captee. I Wu aware tiew e mniflcart paties for KWting tale SIGNATURE OF PRINCIPAL EXECUTIVE

Waonl, ciduing t posebtity d lin ad Iirsorent r knowirng vhlIks.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference al alhtchtents here)

No dosed mode operation. The following injections occured: 1. PCL-222 (max. calc. conc. was 0.067mg/I-limit 0.1 00mg/L) 2. CL-363 (max. calc. conc. was 0.01 1mg/L-limit 0.100mg/L) 3. PCL-222/PCL-401 (max. cac. conc. was 0.023mg/L-limit 0.100mg/L) 4. H-130M (max. caic. conc. was 0.030mg/L-limit 0.050mg/L) 5. H-130M (low detection level analytical method was 0.040mg/L-limit 0.050mg/L)

EPA Form 3320.1 (REV 3199) Prev~~~~~ous editions may be used Page 1 of 2~~~~

EPA Form 3320-1 (REV 399)

Previous edftbns may be used Page 1 of 2

PERMITTEENAME/ADDRESS Indudd Fadlt Nam4ocaton fDiffemnt Name TVA - SEQUOYAH NUCLEAR PLANT Adres ss-P0. BOX 2000

___{NIE OEE---=-------

_SO D

-DASY_ TN 3738_4 __

.Ld&_ VA - SEQUOYAH NUCLEAR PLANT_

.c_

KonAILLOXW.UNT_

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR (SUBR 01)

I TNO 2G450 1

F - FINAL I

PERMIT NUMBERI lDISCHARGE NUMBER]

DIFFUSER DISCHARGE Form Approved.

OMB No. 2040-0004 1 EFFLUENT I

MONITORINrGr PERIODf l YEAR MO DAY IYEAR MO I DAY ATTN: Stephanie A. Howard i

From 03 06 I 0 I To 03 06 30 NO DISCHARGE By NOTE: Read Inshructons before compleing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE

~~~EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE - C. RATE OF SAMPLE 0.4 62 0

30/30 CALCTD CHANGE MEASUREMENT 82234 1

0 0

DEG C

TI A W ITP)

EFFLUENT GROSS VALUE 1

HR

~

j TEMP. DIFF. BETWEEN SAMP. &

SAMPLE 2.1 04 30/30 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1

5 0

W jg DEG. C.

EFFLUENT GROSS VALUE DEG.C.

SAMPLE MEASUREMENT SAMPLE MEASUREMENT.

-2.~~~~~~~~~~~~~~~~~~~~~'.

SAMPLE BORON, TOTAL SAMPLE

<0.2

<0.2 1

1/30 GRAB MEASUREMENT 19 1/0 GA 01022 1

0 0

IPMV 7

. A 'IG___

_IL

_ ~

EFFLUENT GROSS VALUE

~

-E N

SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify urpeaty of low t hi dowsnsrt and OM atsdwneris were prepad uder my I

TELEPHONE DATE

-rctionorsprvsioninam with a system desged to "mm ti qualiIdr 0.ea l

J o

I Richard T. Purcell Prqperty gatherdwid evaue to hmation xbittedo Based orn my iquiry of te Person of liWormafbn lhe tation submitted Is. to the best of my nowleea wi tre.

Ating Environmental Supervisor 423 8434700 03 07 14 SIte Vice President stcmsie ad ooy~wl. I am aware mm t ree sgficar penalies for submiN false SIGNATURE OF PRINCIPAL EXECUTIVE I

TYPED______________

OR PRNTEDhlIortion cling toi possbity of f'mUsd bipnmnot for kig vlt OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED

___I_

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference si attachments here)

CCW data for June 2003 is attached. Quarterly veliger monitoring Information Is attached.

EPA Form 3320-1 (REV 3199)

Previous editions may be used Pag 2 of 2

CCW CHANNEL Date/Time Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst 06/1612003 0900

<0.5 mg/L 06/19/2003 1942 JBR CCW TRENCH Date/Time Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst 06/16/2003 0905 0.5 mg/L 06/1912003 1905 JBR

SQN Forbay Clam Graph Sequoyah Nuclear Plant Forebay Clam Densities 6000

d.

26.0 24.0 50004-l j

+22.0 20.0 Mean# of Corbicula/m3 18.0 4000

-4Water Temp. (C) 16.01 14.0 7in3000

.1 2

.~~00 12.0 EO a..Il0 E

E

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

d, z

2000 80 6.0 1000 4.0 2.0 0

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~0.0 Sample Date Page 1

SQN Forbay ZM Graph Sequoyah Nuclear Forbay Zebra Mussel Densities 200 180 -

30.0 4an I S.

IUL 140-

.0 U

X.

120 t0 c 100-

1 e D CLs 80

.0 80-N 46 i,

60 D

E z

40-20 -

r-I-

25.0

- 20.0 2

cmi l_

Mean # of ZM/m3

-15.0 E

1

% Settlers

-Water Temp. (C)

-10.0

5.0 0.0 I

.1.

I I

I n

I I

V I

I I

.I Ma~~~~~~~~~~~~

A.$

I 11X1100~0 1

Sample Date Page 1

Sample Mean# of Water Sample Mean# of Water LOCATION SU LOCATION SAMPLE Date Z

% S Temp. (C)

Date Corblculalm' Temp. (C)

TYPE COLLECTED BY 4/8/03 4/8/03 4/10/03 4/14/03 4/17/03 4/21/03 4/24103 4/28/03 5/1/03 5/5/03 5/9/03 5/12/03 5/16/03 5/19/03 5/22/03 5/28/03 5/29/03 6/5/03 6/6/03 6/9/03 6/11/03 6/16/03 6/19/03 6/23/03 6/25/03 6/30/03 0

0 0

59 0

0 126 0

10 0

0 13.0 4/8/03 0

13.0 4/8/03 0

12.0 4/10/03 0

15.0 4/14/03 0

15.0 4/17/03 0

16.0 4/21/03 0

15.0 4/24/03 0

19.7 4/28/03 0

18.0 5/1/03 0

19.0 5/5/03 0

19.0 5/9/03 0

18.0 5/12/03 0

19.0 5/16/03 0

19.0 5/19/03 0

20.0 5/22/03 0

23.0 5/28/03 0

21.0 5/29/03 0

23.0 6/5/03 0

22.0 6/6/03 0

24.0 6/9103 0

23.0 6/11/03 0

24.0 6/16/03 50 23.0 6/19/03 0

23.0 6/23/03 0

24.0 6/25/03 0

24.0 6/30/03 31 0

68 0

0 0

0 379 213 144 720 5,248 2,074 129 83 220 1,976 132 1,792 333 1833 2,991 955 1832 13.0 13.0 12.0 15.0 15.0 16.0 15.0 19.7 18.0 19.0 19.0 18.0 19.0 19.0 20.0 23.0 21.0 23.0 22.0 24.0 23.0 24.0 23.0 23.0 24.0 24.0 Intake Intake Intake Intake Intake

.Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Intake Forebay QUANT Jones-Brier/Lehman Intake Forebay QUANT Wanda Allen/Ron Lowery/Jones-Brier Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Intake Forebay QUANT Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Ron Lowery/M.G. Beavers Ron Lowery/R.D. Nunley Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Ron Lowery/M.G. Beavers Ron Lowery/D. Angel Ron Lowery/D. Adcock Ron Lowery/E. Childers Ron Lowery/J.I. Childers Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery Wanda Allen/Ron Lowery

PERMITTEE NAME/ADDRESS jnc& FeclivN&me4oclltn ifDffent

-iM TVA - SEQUOYAH NUCLEAR PLANT Addmss P.O. BOX 2000 JNTEROFFICE SB adly TVA-.SE2UQAH NUCLEAR P LT_

Loca ton _AMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR Foi DISCHARGE MONITORING REPORT WDMR)

(SUBR 01)

Ch t

TN0264550 J

101 T I F - FINAL I

PERMIT NUMBER

[ DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 m Approved.

MB No. 2040-0004 I

MONITtRING PERIOP IYEARI MO I DAY I I YEAR I MO I DAY EFFLUEN,T NO DISCHARGE LI '-

NOTE: Read Instructions before completng this form.

From I, 03 0

01 lTo 031061 30_I COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference e a achments hem)

An unscheduled whole effluent toxicity (WET) test was performed June 15-20, 2003 due to H-1 30M Injection. Report Is attached.

EPA Form 3320-1 (REV 3/99)

Pie Wous editions may be uSed rage oj 1 EPA Form 3320-1 (REV 3199)

PTSVOUS ed/tl=n may be sed Page

July 9, 2003 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, H130M SPECIAL STUDY, JUNE, 2003 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected June 15-20 showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were >

100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples and UV treated samples was not sufficient to jeopardize statistical validity.

Call me at (256) 386-2755 if you have any questions or comments following your review of the report.

Cynthia L. Russell Biol6gist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment):

Files, ER&TA, CEB B-M SQN Hl3OM-June 2003m

ZA, TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: July 9. 2003

1.

Facility / Discharger: Seauoyah Nuclear Plant / TVA

2.

County/ State:

3.

NPDES Permit #:

4.

Type of Facility:

Hamilton / Tennessee TN 0026450 Nuclear-Fueled Electric Generating Plant

5.

Design Flow (MGD): 3266

6.

Receiving Stream:

Tennessee River (TRM 483.6)

7.

1Q10:

2.992.4

8.

Outfall Tested:

101

9.

Dates Sampled:

June 15 - 20. 2003

10.

Average Flow on Days Sampled (MGD):

1585.5. 1592.5. 1557.0

11.

Pertinent Site Conditions:

H130M was being injected from June 16-22, 2003. The dates and times for the injection of H130M are provided below. The resulting H130M concentrations are summarized in Appendix B, along with other chemicals in use during sampling.

Injector Location Date/Start Time (EDT)

Date/ Ending Time (EDT)

Essential Raw Cooling Water J

(ERCW)-Bravo Train

_June 16,2003/1010*

June 17,2003/0840*

June 17, 2003/0950*

June 18, 2003/0830*

June 1, 2003/0940*

June 19,'2003/0910*

Raw Cooling Water (RCW)

June 19, 2003/1605 June 22, 2003/1425

The break between starting and ending times allowed for the back washing of ERCW traveling screens and strainers.

12.

Test Dates:

June 17 - 24. 2003

13.

Test Type:

Short-term Chronic Definitive

14.

Test Species:

Fathead Minnows (Pimephales romelas)

Dayhnids (Ceriodaphnia dubia)

15. Concentrations Tested (%/):

Pimephales promelas:

Outfall 101: 10.98. 22.43.9.72. 100 Intake: 100 UVtreatedOutfall 101: 10.98.22.43.9.72. 100 UY treated Intake: 100

16.

Permit Limit Endpoint (%): Outfall 101: IC25 = 43.9%

17.

Test Results:

Outfall 101: Pimephales promelas: ICZ > 100 Ceriodaphnia dubia: IC, > 100 UV treated Outfall 101: Pimephalespromelas: IC,5 > 100

18.

Facility

Contact:

Phone #:

Ann Hurt (423) 843-6714

19.

Consulting / Testing Lab:

Environmental Testing Solutions. LLC

20.

Lab

Contact:

Phone #:

21.

TVA

Contact:

Phone #:

Jim Sumner (828) 350-9364 Cynthia L. Russell (256) 386-2755

22. Notes: Outfall 101 samples collected June 15 - 20, 2003, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were> 100 percent.

Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at..

Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-tieated and UV treated samples, suggesting that pathogenic bacteria were not present.

2

METHODS

SUMMARY

Samples: -

1.

Sampling Point: Outfall 101. Intake

2.

Sample Type: Composite

3.

Sample Information:

Date Date Date (MM/DD/YY)/

(MMDDIYY)/

Arrival Initial (MMIDD/YY)/

Sample Time (EDT)

Time (EDT)

Temp.

TRC*

Time (EDT)

ID Collected Received (C)

(mg/L)

Used 06/15/03 1257 to 06/17/03 1422 101 06/17/03 0946 0.4t

<0.10 06/18/03 1330 06/1/03 115 06/17/03 122 Intake 06/16/03 1235 06/17/03 0946 0.4

<0.10 06/18/03 1330 101 06/1/03 102 06/19/03 0939 0.7t

<0.1 06/1/03 1336 06/18/03 1302

_____06/20/03 1400 Intake 06/1/03 1248 06/19/03 0939 0.4

<0.10 06/19/03 1336 06/1/03 1268t 06/21/03 1400 101 06/20/03 1156 06/21/03 1004 O.9t

<0.10 06/22/03 1342 0623/03 1338 06/21/03 1340 Intake 06/19/03 1233 to 06/21/03 1004 2.1

<0.10 06/22/03 1342 06/20/03 1133 06/23/03 1338

TRC = Total Residual Chlorine tCollected in two 2.5-gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation:

Samples from Outfall 101 and the Intake were warmed to test temperature (25.0 1.0C) in a warm water bath.

Aliguots of Outfall 101 and Intake samples were UV-treated through a 40-watt Rainbow Lifeguard UV Sterilizer for 2 minutes.

3

Pimephales vromelas Ceriodaphni dubia Test Organisms:

1. Source:

Aquatic BioSystems. Inc.

In-house Cultures

2. Age:

23.5-25.5-hours old

< 24-hours old Test Method Summary:

1. Test Conditions:

Static. Renewal Static. Renewal

2. Test Duration:

7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic

3. Control / Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

4

5. Organisms per Replicate: 10 10 1

6) Test Initiation: (Date/Time)

Outfall 101 UV Treated Outfall 101 06/17/03-1422 EDT 06/17/03-1440 EDT 06/17/03-1314 EDT

7) Test Termination: (Date/Time)

Outfall 101 06/24/03-1440 EDT UV Treated Outfall 101 06/24/03-1505 EDT 06/24/03-1300 EDT

8) Test Temperature: Outfall 101: Mean =24.90C (24.3-25.50C)

Mean = 24.70C (24.3-25.60C)

Test Temperature: UV-Treated Outfall 101: Mean = 24.90C (24.3-25.70C)

9. Physical / Chemical Measurements:

'Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and Rost-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software.

McKinnevville. CA).

4

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted June 17-24. 2003 using effluent from Outfall 101.

Test Percent Surviving Solutions (time interval used - da

_s),

(% Effluent) 1 2

3 4

5 6

7 Control 100 100 100 100 100 100 100 10.98%

100 98 98 98 98 98 98 22%

100 100 100 100 100 100 100 43.9%

100 100 100 100 100 100 100 72%

100 100 100 100 100 100 100 100%

100 100 100 100 100 100 100 Intake 100 100 100 98 98 98 98 Mean Dry Weight (mg)

Test Solutions (replicate number

(% Effluent) 1 2

3 4

Mean Control 0.886 0.778 0.763 0.915 0.835 10.98%

0.799 0.739 0.711 0.833 0.771 22%

0.970 0.806 0.863 0.960 0.900 43.9%

0.941 0.964 0.964 0.968 0.959 72%

0.890 0.925 0.942 1.027 0.946 100%

0.944 0.915 0.952 0.816 0.907 Intake 0.843 0.923 0.798 0.919 0.871 IC25 Value: > 100%

Permit Limit: 43.9 Calculated TU Estimates: < 1.0 TUc*

95% Confidence Limits:

Upper Limit: NA Lower Limit: NA Permit Limit: 2.3 TUc

TUa = 100/LC50: TUc = 100/ IC25 5

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted June 17-24. 2003 using effluent from Outfall 101.

Test Percent Surviving Solutions (tirne interval used - days)

(% Effluent) 1 2

3 4

5 6

7 Control 100 100 100 100 100 100 100 10.98%

100 100 100 100 100 100 100 22%

100 100 100 100 100 100 100 43.9%

100 100 100 100 100 100 100 72%

100 100 100 100 100 100 100 100%

100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

'°/ Effluent)

-Data (replicate number)

(uent 1

2 3

4 5

6 7

8 9

10 Mean Control 30 30 23 27 28 30 26 29 24 29

27.6 10.98%

27 29 24 30 28 30 33 28 31 31 29.1 22%

31 30 34 29 37 28 28 30 32 28 30.7 43.9%

35 31 34 31 32 35 32 29 33 36 32.8 72%

34 29 35 30 36 31 33 33 30 36 32.7 100%

37 34 32 33 35 36 35 37 31 35 34.5 Intake 28 29 33 31 29 30 29 27 29 33 29.8 IC25 Value: > 100%

Permit Limit: 43.9 Calculated TU Estimates: < 1.0 TUc*

95% Confidence Limits:

Upper Limit: NA Permit Limit: 2.3 TUc Lower Limit: NA

TUa = 100/LCso: TUc = 100/ IC25 6

TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)

3.

Results of a Pimephales promelas Chronic 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted June 17 - 24. 2003 using effluent from UV Treated Outfall 101.

Test Percent Surviving Solutions (time interval used - days

(% Effluent) 1 2

3 4

5 6

l 7

Control 100 100 100 100 100 100 100 10.98%

100 98 98 98 98 98 98 22%

100T 98 98 98 98 98 98 43.9%

100 100 100 100 100 100 100 72%

100 98 98 98 98 98 98 100%

10 100 100 100 100 100 100 Intake 100 100 l100 10 100 100 100 Test Solutions Mean Dry Weight (mg)

(% Effluent) 12i(replicate number)

(_

1 3

4 Mean Control 0.722 0.769 0.830 0.923 0.811 10.98%

0.840 0.831 0.912 0.944 0.881 22%

0.974 0.880 0.837 0.942 0.908 43.9%

0.822 0.853 0.945 0.898 0.880 72%

0.909 1.045 1.009 0.971 0.983 100%

1.002 0.938 0.886 0.745 0.893 Intake 0.860 0.916 0.924 1.048 0.937 IC25 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

95% Confidence Limits: NA Upper Limit: NA Permit Limit: NA Lower Limit: NA

TUa= 100/LC 5o: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see A Dendix A and D)

Species Date -

Time Duration Toxicant Results (IC25)

Pimephales promelas June 17,2003 13461 7-days KCI 1602.2 mglL Ceriodaphnia dubia June 04, 2003 1310 7-days INaCI 1068.7 mg/L 7

P}IYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pimephalespromelas and Cerlodaphnia dubia Tests, Sequoyah Nuclear Plant EMuent (SQN), Outfall 101, June 17-24, 2003.

Test Sample ID Temper tre C)

Dissolved O ygen (mglL) pH U.)

Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pmhos/cm) (mg/L CaCOs) (m%/L CaCO3) Chlorine (mglL) 25.3 24.6 7.6 7.3 7.72 7.56 301 60.9 84.1 Control_

25.1 - 25.5 24.3 24.7.

7.6 7

1.7 6.5 - 7.7 7.63 - 7.30 7.36 - 7.73 290 -

317 60.2 -

61.2 82.8 -

S4.8 10.95%

25.3 24.6 7.7 7.3.

7.71 7.56 -

287 25.0 - 25.5 24.3 - 24.7 7.5 -

7.9 6.5 -

7.6 7.67 - 7.76 7.44 - 7.69 276 -

301 22%

25.3 24.6 7.7 7.3 7.71 7.56 276 22%

25.0 25.5 24.3 - 24.7 7.5 -

7.9 6.6 -

7.7 7.67 - 7.75 7.46 - 7.69 265 - 24.

25.3 24.6 7.8 7.4 7.67 7.55 243 43.9%

25.0 - 25.5 24.3 - 24.7 7.6 -

7.9 6.7 -

7.3 7.64 - 7.71 7.43 - 7.67 233 -

243 25.2 24.6 7.8 7.4 7.62 7.55 206-

_72%

24.8 - 25.5 24.3 - 24.7 7.6 -

7.9 6.7 -

7.3 7.56 - 7.69 7.48 7.67 199 -

215

,,j 100%

25.3 24.6 7.8 7.4.

7.56 7.51 166 57.4 64.0

<0.10 24.7 - 25.5 24.3 - 24.7 7.6 -

8.0 6.9 -

7.6 7.43 - 7.66 7.42 - 7.64 164 -

169 57.1 -

53.1 62.6 - 66.7

<0.10 -

<0.10 Intake 2.2 24.6 7.9 7.4 7.55 7.50 ^

164 57.8 63.3

<0.10 nh

24.9 - 25.5 24.3 - 24.7 7.8 -

8.0 6.8 -

7.8 7.47 - 7.67 7.41 - 7.64 161 -

168 57.1 58.1 62.6 -

64.6

<0.10.

<0.10 Control 25.0 24.4 7.6 7.9 7.72 7.83 301 60.9 84.1 24.5 - 25.6 24.3 - 24.5 7.6 -

7.7 7.7 -

8.2 7.63 - 7.30 7.72 - 7.92 290 -

317 60.2 61.2 82.8 -

34.8 0.98%

25.0 24.4 7.7 7.9 7.71 7.86 287 10 24.5 25.6 24.3 - 24.5 7.5 -

7.3 7.6 -

8.3 7.67 - 7.76 7.73 7.97 276 -

301 22%

25.0 24.4 7.7 7.9 7.71 7.85 276 24.S - 25.6 24.3 - 24.5 7.5 - 7.9 7.6 -

8.4 7.67 - 7.75 7.73 - 7.96 265 -

234 25.0 24.4 7.8 7.9 7.67 7.85 243 I

43.9

24.

25.6 24.3 - 24.5 7.6 - 7.9 7.6 - 3.4 7.64 - 7.71 7.70 - 7.97 233 -

243 72%

25.0 24.4 7.8 7.9 7.62 7.81 206 24.5 - 25.6 24.3 - 24.5 7.6 - 7.9 7.6 -

8.5 7.56 - 7.69 7.67 - 7.93 199 -

215 100%

25.0 24.4 7.8i 7.9 7.56 7.79 166 57.0 63.3

<0.10 24.5 - 25.6 24.3 - 24.5 7.6 -

8.0 7.6 -

8.4 7.43 - 7.66 7.65 - 7.91 164 -

169 57.1 -

58.1 62.6 -

67.0 co.10 -

<0.10 Intake 25.0 24.4 7.9 7.9 7.55 7.77 164 S7;8 63.3

<0.10

________ 24.5 - 25.6 24.3 - 24.5 7.8 -

8.0 7.6 -

8.3 7.47 - 7.67 7.66 - 7.34 161 -

168 57.1 58.1 62.6 -

64.6 0.10 -

<0.10 8

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for the Pimepialespromelas Test, Sequoyah Nuclear Plant Efuent (SQN), UV Treated Outfall 101, June 17-24, 2003.

Test Sample ID Temper tre (C)

Dissolved O ygen (mg/l) pH U

SAJ.)

Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pmhos/cm) (mg/L CaCO) (mg/L CaCO3) Chlorine (mi )

Control 25.3 24.6 7.8 7.5 7.72 7.61 296

24.6 25.7 24.3 - 24.8 7.6 8.0 7.1-7.8 7.61 - 7.77 7.53-7.72 '285 306 10.98%

25.3 24.6 7.8 7.4 7.73 7.56 289 24.6 - 25.7 24.3 - 24.8 7.5 -

8.0 7.0 -

7.6 7.63 - 7.77 7.47 - 7.68 279 -

300 22%

25.3 24.6 7.8 7.4

'7.73 7.55 276 24.6 - 25.7 24.3 - 24.8 7.6 -

8.0 6.9 -

7.7 7.61 - 7.77 7.44 - 7.68 266 - 284 25.2 24.6 7.8 7.3 7.71 7.53 242 24.6 - 25.7 24.3 - 24.8 7.6 -

8.0 6.9 -

7.6 7.S9 - 7.77 7.43 - 7.68 234 - 249 72%

25.2 24.6 7.8 7.3 7.68 7.50 206

.E_______ *24.6 - 25.7 24.3 - 24.8 7.6 -

7.9 6.7 -

7.6 7.57 - 7.75 7.42 - 7.65 200 -

216 100%

25.2 24.6 7.8 7.4 7.64 7.51 167 24.6 - 25.7 24.3 - 24.8 7.6 -

7.9 6.8 -

7.8 7.52 - 7.74 7.42 - 7.64 163 -

170 Intake 25.3 24.6 7.8 7.3 7.61 7.49 163 e_

24.6 - 25.7 24.3 - 24.8 7.6 7.9 6.9 7.7 7.52 - 7.69 7.36 - 7.63 160 -

166 9

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected June 17 - 24, 2003, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present.

10

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1.

Pimephales prom'elas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.

2.

Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1.

Pimephales promelas None

2.

Ceriodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1.

Pimephales promelas None

2.

Ceriodaphnia dubia None 11

PHYSICAL AND CHEMICAL METHODS

1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured using EPA Method 170.1.

4. Dissolved oxygen was measured using EPA Method 360.1.

S. The pH was measured EPA Method 150.1.

6. Conductance was measured EPA Method 120.1.

7. Alkalinity was measured using EPA Method 310.1.

8. Total Hardness was measured EPA Method 130.2.

9. Total residual chlorine was measured using EPA Method 330.5.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g KCI or NaCL.

2. Standard Toxicant:

Potassium Chloride (KCl crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

12

REFERENCES

1. NPDES Permit No. TN 0026450

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).

13

Sequoyah Nuclear Plant Biomonitoring June 17 - 24, 2003 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling,

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-June 20, 2003 15

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-June 20, 2003 t~&a~t~#%iTowerbrom>

GL22 PCL-401 Cuposat Date mgL rcLog TRC Sopat Copolymer tDAD Qta Azole 4r~~

01/31/2000 4..021,

.0 t

DS W

O D

<000 B$ O s° 0.009 02101/2000 0.110.028 02I02/2000 \\y g>b 0.028 02.4 0.009 oo&

02103/2000 08 0.009 t

S 02/04/2000 0.006 0.009 g

0.109 t

02105/2000

~

< 0.002 0.009-02106/2000.Sz 4

s-

< 0002

t0!2W 0.009

~

t tt 07/26/2000 07

.1 07/27/2000 0.0190.01 07/28/2000 >0 t..

.F.'8 003' 0.018 000>

0.108 07/29/2000

< 0S0088 0.019 07/30/2000

< 0.0076 0.019 07/31/2000

_<0.0152 0.1 08/01/2000

<00141 0

0.019 G00&

12111/2000 0

0.020 ___,O.{.

1211212000 0.0092 G~V 0.020 9S 12113/2000

< 00120 vt&sk 0.020 12114/2000 7

2,~*r

<00087 0.020-12/115200OOO fAi 0.0120 0.020 GGi 12/16120~<

0.0036 Vozr 0.020 12117/200

<00036 000-<

08/26/2001 t*

~~

0.017 9Pff0 OV%:

0.021 iGtQC" 08/27/2001 A

<0.0096 0.021 04W$

08/28/2001

_ 4 jtt<0.0085 2tb6TC 0.02 1 08/29/200 1 ~-~

'*S<0.0094

$5?"

0.020

'00QE 08/30/2001

<0.0123

~tMZ 0.021-08/31/2001

<0.005 0.020-11/26512001 4 2'<0.0044 11/6100

<.01 9-.--2 240.02 11/27/2001

'4~X b0037 Xop.

0.0 19-11/28/2001 ~

~

<0.0089 ~'GQ2~

0.019 G9Y 11/29/2001 0.0132

'0.02 e 0t 11530/2001

<0 00043

£t4 0.02 12109/2001

<0.0042 12110/2001

<0.0042 vkt 12111/2001

<0004 12iv2oi01L~

0.0128 tZ 0.02 00$

12113/2001

~

<0.0088

.04<

0.02-12/14/2001 1¶ 0.0134 CrWQ~Z 0.02 J~07

-'W 16

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-June 20, 2003 17

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-June 20, 2003 18

Sequoyah Nuclear Plant Biomonitoring June 17 - 24, 2003 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

~ ~ ~~~~~.

l

-X I

BIOMONITORING CHAIN OF CUSTODY RECORD Page

_________________________________________________________ I.

1 I

-. ---.

Client: TENNESSEE VALLEY AUTHORITY Project Name: SEQUOYAH NUCLEAR PLANT i

P.O. Number: PO BOX 2000 Facility Sampled: SEQUOYAH NUCLEAR PLANT_

NPDES Number. TN 0026450 ENVIRONMENTAL TESTING SOLUTIONS 351 DEPOT STREET ASHVILLE, NC 28801 PHONE:

828-350-9364 FAX:

256-386-2963 EMAIL: CLRUSSEL2@TVA.GOV

(

pef)

UPS Other (specify),,

BUS a

__UPS_.

e Cllent TI i General Comments:

CLLicdJI AAA16 I

tL1 F

1 9~~~~~~~~~

teuM A.

runny D.

WAKII'A K Al I FN Collection Date/Time Container Rain Event?

Field Identification I Grab/

Ship.

(it grab, list date/time under Number &

Flow (Mark as Appropriate)

Sample Description Comp.

Temp.

'End')

Volume (MGDo (IC)

Collected

)

Start End Yes If Yes, No Trace

~~~~~~~~~~~~~~~~~~~~~~~~~~~Iches SQN-DSN-101-TOX

-GR 4

6/15/03 It1 6/16/03)

(2)25 GAL SQN-INT-TOX 3

4 6/15/03 6/16/03 z

GAL

-~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Sarple Custody - Fill In From Top Down Relinquished By (Signature):

Date/Time Received By (Signature):.

DateMme 6/16/03 elb 4ZXq 0bi'lo Febes nvOq(t-a O

1a Fabc. *

.-rl-o?

q~

, I I

Instructions: Clents should fill in all areas except those In the 'Laboratory Use* block. Biomonitoring samples are preserved by storing them at 4eC and shipping them In Ice. The hold time for each sample Is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship In such a way that the laboratory will receive the samples with ample time to Initiate testing within that time frame.

Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

.~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~nuhnnV'1i~n1nIhI YRLIA PbP0_rfz~~~~

VA &c

BIOMONITORING CHAIN OF CUSTODY RECORD Page l of /

U I

Client: TENNESSEE VALLEY AUTHORITY Project Name: SEQUOYAH NUCLEAR PLANT P.O. Number: PO BOX 2000 Facility Sampled: SEQUOYAH NUCLEAR PLANT_

NPDES Number TN 0026450 Collected By: WANDA K ALLEN ENVIRONMENTAL TESTING SOLUTIONS 351 DEPOT STREET ASHVILLE, NC 28801 PHONE:

828-350-9364 FAX:

256-386-2963 EMAIL: CLRUSSEL2@TVA.GOV UPS Other (specify)-.

Bus Client CTI $..

General Comments:

.tj+ 1ej i

"A'D, he fl

,..~_

Collection Date/Time Container Rain Event?

Field Identification I Grab/

Ship.

(If grab, list date/time under Number &

Flow Sample Description Comp.

Temp.

'End')

Volume (MGD (Mark as Appropriate)

(C)

Collected I

Start End I

Inchs Trace SON-DSN-101-TOX G3R" 4

6/17/03 6/18/03 (2) 2.5 Imp I@

H. V, GAL SON-INT-TOX 4

6/17/03 aq 6/18/03 Sample Custody - Fill In From Top Down I

I.

i,4 I

Relinquished By (Signature):

Daterime Received By (Signature):

DateMme l~~~~~~tL ~~~6/18/03 arAil-3\\a aG.& amQTo V-red

_0__

Ob-l-o3 O59 fI'/{z 1

ay E7T5 Instructions: Clents should fill In all areas except those in the Laboratory Use' block. Blomonitorlng samples are preserved by storing them at 40C and shipping them In Ice. The hold time for each sample Is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to Initiate testing within that time frame.

Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page l of l ENIOMNA TESIN Client: TENNESSEE VALLEY AUTHORITY E NIOM TAO S

<1Ž) UPS Bus Client CTI $

SOLUTIONS t

Project Name: SEQUOYAH NUCLEAR PLANT 351 DEPOT STREET Other (specify):-

ASHVILLE, NC 28801 P.O. Number PO BOX 2000 PHONE:

828-350-9364 Facility Sampled: SEQUOYAH NUCLEAR PLANT FAX:

256-386-2963 EMAIL: CLRUSSEL2@TVA.GOV General Comments:

NPDES Number: TN 0026450 4 Cod2u 5

Collected By: WANDA K ALLEN Collection Date/ime Container Rain Event?

O i

Field Identification Grab/

Ship.

(If grab, list date/time under Number &

Flow l.

Sample Description Cm.Tp.'End')

Volume (MGD (Mark as Appropriate)

~

___________________(OC)

.Collected J

-II~

Start End l

i Yes If Yes, No Trace i MO.

_________Inches N~ie SQN-DSN-101-TOX 8RA 4

6/19103 6/20/03 (2)2.5 MAl=

E GA X=.

.j tX SQN-INT-TOX 4

/1036/0/103

/3.

c~4 Sample Custody - Fill In From Top Down Relinquished By (Signature):

Date/Time Received By (Signature):

Date/Time 4f4!4S-

~6/20/03 0O6-2-0r!

VtS Fete_

loo4 l _

6-z-o Ick sj Instructions: Cents should fill in all areas except those in the Laboratory Use block. Qmhitoring samples are preserved by storing them at 41C and shipping them In Ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please cllect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

I' ~ ~

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-~ Environmental Testing Solutions. LLC Paze I of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephales promelas Client: TvYh Facility:

J P1 pM A

iAIJT NPDES #: "M'-

Project #: _

4J Dilution preparatwn nformation:

Comments:

Dilutio pr()

Nl.9b-it 1Z 1.Z 100 Effluent volume (mL)

Z A.

&cio all o

Diluent volume (mL) In(O.4 D I O

12Z 5bO 0_

Total volume (mL) 2 ZC ZO O

C Test organism in Test information:

Organism age:

a

- hopes Old Randomizing template:

6ue_

Date and times organisms C-13 1m ico(cj Incubator number:

were born between:

aU Organism source:

Pte$ EPRMA Oi-ts Artemia lot number.

6W Transfer bowl information:

pH =

Temperature

'C Total drving time:

_q__e_

__ 00 _

1.1 Date/ Time in: cL,.

%oG 60 Average transfer volume:

DateITime out:OL 10 Oven temperature:

IC

' C.

Daily feeding and reneval information:

Day Date Morning Afternoon Test nitiation, Control water Sample numbers Analyst feeding feeding renewal, or batch used used time time termination time M

tt______

X o oW-n u

~

~q 2-030(.

S1 UF

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A="nce aitr Summarv of test ndpoints:

% Mortality:

0').

SI20%

1-day LCso

> I l K....

Average weight per initial larvae:

5 NOEC lOEC Avcrage weight per surviving larvae:

0.k 0.25 mgam LOEC 1 I00%

IChV I WISi 10'

Environmental Testing Solutions, LLC Page 2 of 6 Species: Pimephalespromdos Client: -2VA-eO&I Date:

6L7-lta tWNreATeb Survival and Growth Data Day CONTROL lo.'87 ZZ7^

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I~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

rironmental Testing Solutions, LLC Page 3 of 6

. 8.

Species: Pimephalespromelas Client: IVA - i'-Qd i-~~~~~~A 11 oWs4 Date:

,al11 eb.

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-aM7 M

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nmcntal Testing Solutions, LLC Page 4 of 6 Species: Pimephalespromelas Client: T Se(NY NA Date:

Q6-1 1 -CE23 Survival and Growth Data Day a MTAe Y

Z AA BB

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' I Larvae weight (mg) - A-B Q8 5

g.%%64 4,.t h-qla CCalculations and data reviewed:

'-A Comments:

I Environmental Testing Solutions, LLC Chronic Whole Emuent Toxicity Test (EPA-82 1-1-02-013, Method 1000.0)

Species: Pephalesprontlas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sque"Is Nucie. Plat Nentrasted Client:

Test data June 17-24.2003 Prdc umber 744 Revelwarl by.

CacpolrUe (

t Reqk1k toIdthi soler of Plan smber t bam AJ' tas weighl (mg]

H

Pa.

Larvae ter.

wtight (m0 We4g1 talent ember lMa srstue" bt-s weibi (mg)

C fest oft Pa raust eduion bram barre w.ght (m,

-Ae (lbm) wr ahlss 5) mn" 11%)

A 10 10 14.74S 23.600 1.55 O.lt55 Cantrl B

10 10 14.653 22.440 7.782 0.7712 100.0 0.352 9.1 No applicable C

10 30 14.933 22.560 7.627 0.7627-D 10 10 IS.04S 24.190 9.145 0.9145.

E 10 10 15.069 23.060 7.991 0.7991 10.96%

F 10 10 14.56S 22.9I0 7.315 0.7385 97.S 0.7705 7.2 7J 0

30 10 55.039 22.150 7.111 0.7111 10 9

15.049 23.3t0 1.331 0.8331 I

10 t0 15.249 24.950 9.701 0.9701 22%

.1~

~

10 10 14.861 22.920 3.059 038059 100039 11%

10 10 14.945 23.570 3.62S 0.8625 L

10 10 14.975 24.570 9.595 0.9595 M

10 10 15.019 24.430 9.411 0.9411 43.9% ~

N I10 10 14.760 24.400 9.640 0MO4 100.0 0.9S93 1.i

-14.9 0

10 10 14.697 24.340 9.643 0.9643 P

10 10 14 3

-24.510 9.677 0.9677 10 10 15.266, 24.170 5.904 0S904 72%

R 10 10 15.053 24.300 9.247 0.9247 72%

S 10 10 14.842 24.260 9.411 0947 100.0 0.9461 62

.13.3 T

10 IS.396 25.670 10.274 1.0274 U

0 10 14646 24.090 9.444 0.9444 100% ~

V10 10 14.735 23.110 9.14S 0.9145 100897019-.

100%

W 10 10 14.697 24.220 9.523 0.9523 100.0 e:9067 ii X

10 S0 15.1 13 23.270 1.157 0.1157 U

10 10 14,636 23.070 1.434 0.3434 100%

ntake V

10 10 1.693 23.920

.227 0.27 100% Intake V

10 90 14.1

.22 60 7.979 0.7979

.0 3U 7

X 10 10 15.037 24.230 9.193 0.9193 Outbil 101:

I)unnetts MSO value:

PMSM:

Inisket Dunnctta MSD value:

PMSD:

0.1046 12.5 0.0946 11.3 MSD -

Mnion nSinluficant Diffience PMSD Prcat MiniurnSigniicant Diffraence PMSD ise rasurr of et precision. The PMSDis ihe niinaanprceu diffnce beaw Len he ol a

dbnwht mC be decled atalisticlly signalicant in a whole effluent toxicity tat. On inge. a significant difference ccus fr wAr1restal Testing Solutions LlC droic toxicity tests when l toxicaot reduces Pinuphsles growth by 16. % fiwn he cnnl(daltennined rth gdienc locia teuingJ.

Lower PMSD bound dewiiaend by UsEPA (lOdt paccrtile) - 9.4%

Lpper PMSD otund ddertnined by USEPA (Sh percentile) - 35 Thc lower and upper bounds were calculated by thc LEPA using 205 tests conducted from 19 labnaties ftr Pirrphics Vwth in dlvalo reabenc toxicant tests.

USEPA 2000 Ehdet b ing nd Accounting fr Method Variability in Whiole Emuent Toxicity Applications Uider the National Pollutat Dischge Elinintion Paogrmt EPA-33-R-0403. W &nt4'w'mtsPmt" Aaency.

Cincinnati. Ol.

Environmental Testing Solutions, LLC Statistical Analyses La1 Fat cg 5

d r

DSurvirs tag-i "Giwtk 21Duac

&17103 Tat ID PpFnICL saa ID Sequayh Nudcer PLa OUCU 101 D DUc 24103 Lab k rISamuT m sob5 m supa 91we DJAI.Lach 4izn Rap-II=p Da POceot CIM=41COA.821l

3)

Tt Spda:

Wp.Mmqual m

Proad Coma%

I 2

3 4

DCmn 0"

0.772 17627

.3145

l.

0.7991 1.7385 0,711 t1 22 031701 o.059 eJ6D3 0.9595 as 0341!

.l40N 1.964 77 72 Q.94 OS247 41S 10274 10 09444 6314 G3M 157 Trans&-ml Uubwa med 1.TaIZd Istank Cc%

Mn N-Mom MGM Min Max CV%

N

,Jtat CrHI" M5D Mm.

.Mm.-

DCamel 0.1352 1.0000 0152 0.7627 03145 9.092 4

0162 10000 10m

.7705 0.924 0.7705 0.7111 o31 7222 4

1.43 2410 I046 0.362 1.0000 22 1.99 1.0770 199S 0.8059 0.1101

.791 4

1.4E1 2.410 C046 01862 1.0000 43S 63593 1.1425 0.9593 0S411 0.9677 1.275 4

.22 2.410 0.1046 0.1862 1.0000 72 09461 1.1327 0.9461 01904 1.0274

.160 4

2JS4 2L410 1.046 0.0862 1.0000 100 0S067 1.0056 09067 01157 0.9113 6.929 4

-.647 2.410 C1046 0.362 1.0000 Aiiary r.t Statnfic CrU13 SkeW Ket mpiboWil Tat indicates mal disibun 0.01) 0.95224813 0184 4139U4902 *.10445676 Sudews Tet indicates qua mances (p 0.241

_.764160153 15.07631706 Hypasis Tdat l-1aL 10.51 1NOEC LOEC ChV TU MSIU MSDp MIS MSE Ftrab dr Owams Teg to I 100 1

0.104593279 0.t1227669 0.020415TI 0.00376708H 0.003256632 5.15 Tresabrus vs DCawl Ll lpala.

201 Rauuiples)

Few so

95. CLIZxpl Skrw COS

'100 ICI 300 ICIS

'300 1.0 K20

'00 0'

lCU

'100 0.8 950

'o100 0.

"0.

0.5 1

0.2 0.1 0.0.

"'I

.01

.. e*.---

.0.2

.0.3 0

20 40 s0 so 100 120 Dos %

DeseaRapoane Plot 1.2 1~

0.8 C.2 1.bi. 1.05 Love1 of mgftlcfll O4 I

9 A

i 0

a qnOV6t7-Wdaazfr

Environmental Testing Solutions, LLC Statistical Analyses La 1 lish Growth and Survival Test.7 Day Growth Stat Datc 6l7103 Tat D.

PpFRCP.

Sangle lD.

equayah Nucdes Plant. Wake End DstAe 6/4203 Lab D.

T-Enr. T in& Soltions SACleWpc D R-Discbuze Mokeing Rept Snvi Dta IuoCOL CHRONC(PA421-R013)

Tet Spcies PFzpes`

Cane-%

1

2.

3 4

DwCtvol 0.355 77n 0.1627 0.9145 100 L0.434 0.9227 0.7979 0.9193 Traasona: Untransfarmed I-Tailed Conc-%

Mean N-Mean Mean Mi Mal CV%

N t-Stat CriUcal MSD OCowxol

(.3352 1.0000 0.8352 0.7627 0.9145 9.09t 4

O0 0.370t 1.0426 0.8703 Q7979 0.9227 6.99 4

.0.731 1.943 0.0946 Auxiliary Tests Statistic Critical Skew Kurt Shapi-Wil Test iicats noamsl dislnbucion(p>0.01) 0.373248 0.749 4.07104958.2.23744906 F-Tet indicates equI %uinces (p - 072) 1.5591561t 474633456 Hypothesis Test (1-taiL 0.05)

MSDu MSDp MSB MSE 1-Prab dt HowiscedastietTadicatesnosificantdiffer cs 0.09459063 a1 1325163 0.00253472 0.00473914 0.49213776 1.6 Tcetrrntsw OCeatol Dose-Rasponse Plot I,

0.9 0.3 0.7

_10.6.

O 0.5 C3 0.4 I-0.5 0.2 0.1 0

14ail. 0.05 level of significance I

zqnfi6-1-03data-z

....(

Environmental Testing Solutions, LC Page of 7 Environmental Testinf SOlutionS. LLC Pate I of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphnia dubia Client: -T*

Facility:

=4 5

NPDES #: TM) OMZ(o4S Project #:

Dilution preparotion nrmation:

Comments:

Dilutionprep(%)

lo.54 ZZ 4lZ I

Effluent volume (L) 440 1 Rg IlO 4C CC Diluent volume (mL) 1^190.

S 2

SaoD O

Total volume (mL)

Z7 i ZCI>

Zewoo Test organism information:

Test information:

Organism age:

29Aoues pt-b O

Randomizing template:

LU Date and times organisms c-n-c

) c52 To llZ6c Incubator number and were born between:

shelf ocation:

Z Organism source:

IC 10-P-

j YCT batch:

t Z

Transfer bowl information:

pH CO Temperature v It I Selenastrum batch:iOS OS. Z Daily renewal information:

Day Date l

Test initiation, Control water Sample numbers Analyst runewal, or batch used j

used terrnination time H AS I

o O. ni -3 1E\\:b aN-to o O?

'7; a 0 1 o

'J.07

' O{i-1s-lZz 0(011-O 3

L.6 lL Z 2

2 Z195 3 O0n-11 0

/3 0o0

/. CZ 3

10 -

0 1 ZD O Z-636NO I. Il AZ 4

5 az IZ3 L

O-2 O1 6v a62l

.Ob l. 01 A

6 a 2Cc3 1220 Q1 psa2I.dLI. 01 L

7 dbV0 y C3 b~oo Control information:

Accptance criteria Summary of St endponts:.

% of Male Adults:

!5 20%

7-day LC5O

> IC)

% Adults having 3 Broods:

IMS6 z 0%

NOEC

% Mortality:

r oods:

s20%

LOEC Mean OffspringlFemale:

27.

I 25.0 afspn;rml ChV

% CV:

9. 2__

c 40.0 *,s IC25

_1_07

Environmental Testing Solutions. LLC Page 2 of 7 Species: Ceriodaphnsia dubia Client: XrJP A-Se0.Qa AA Date: CL-flO3 CONTROL Survival and Rcproduction Data Replicate number Day

[1 2

I 4

5 6

9 10 I

Young produced

)

L

=

Q l

2 Young produced 0

oN I

Q o I

C)

I c, I c I c,

Adultmortality L

l l

i I l I 1

[

3 Young produced TQ C

C=

Adult____

L j

L.. I t

l

Jl 4

Young produced 3

T 7l

____~~~~

L-5

[

Si~I~

Adult mortality<>

t- - -

Adultmortallty L

__L L

t C -

6 Young produced O

I O

/0 12..

0.

IQ 0l AdultorU.

Ll1 L

l1 7J Young produced JI)(o, 1z 1

13-1 I0o i i2. JT

52.

Totalyoungproduced j

3 Z3 21 E

2 50 2 Z

2q Final Adult Mort.lity W

X for 3" Broods K

I X-l >C l X Concentration:

% Mortality:

I (15 Mean OffspnngFemale:

21 (0 CONCENTRATION:

Survival and Reproduction Data I

Rcplicate number Day 2

3 4

l 5

6 l

7 l

8 I

9 l

1 I"

oungproduced C)

(;C) 1C)@

)l6

[0 1° [

Young rdcAdult mortality L-l 2

Young produced n

o

=

IO.'

Adult mortatlty L

rTC 5

3 Young produced [l)

I l

Q

°L L

d

.Q I

o1 I l Adult mortality [.

l-L

i.

I u

l l

4 Young produced 2LL j1 q

j j3 i S i 1

3 i Adult mortality l I__Il Ll l

I lI

-1 L

ll S

Young produced l ?

-C)

J III I

/I l L

l A3110 Adult mortality L

L L

i L

_ l 1.-.

L,

6 Young produced 1tl 1

j I

C I D 6

I I A

dAdult mortality lLIL l

1

(

i l (

I

+/-

L+/-

7 1Yong produced 1

1Z q

t <0lIn Totalyoung prduced 2

3l l

l 0

I I I

l Funal Adult Mortality IJ.

4,*~ %

I I

I Concentration:

% Mortality:

l 7;

MeanOffsprin enale: I Vzq.I I %Reduction from Control: I-S.t 7 l

Environmental Testing Solutions. LLC Page 3 of 7 Species: Ceriodaphnia dubia Client: -TVA -SPs cQH PAA Date:

6i r-Survival and Renroduction Data CONCENTRATION: -

r-Replcate anmber Day 1

2 3

4 5J6 7

a 10 I

Young produced o

n C) O O Adult mortity.

a I

2 Young produced O

Q O

3 upc IOIcQ I Young I

rd Tce Adult mortallty l

i U

L U j U j t

4 Young produced 4

I L I 3

J Adult mortnity L

L I

lL U l s

Young produced l

310 l1141

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(

A dultmortality L

L-LI T

L C

Young produced l I

L Adult mortality J J ".

I -

__1_

L L L I

j 7

Young produced l

l L

IS J j J J lI~~~~~~~

1 j0 Is ~

1T 1 1 VA Total young produced 5

- l i l Final Adult Mortality l

i l

Concentration:

%Mortality:

Mean Offspring/Female:

I l

% Reduction from Control:

1 '..2T CONCENTRATtON: 50--1 {oSurvival andReproduction Data Replicate number Day 1

2 1

3 1

4 f

S 6

171819 10 Il Young produced Q n L I C

.)

l0 ° lO l

Adultm ortality l

L-I U I U

I l -

Ll U

U....

2 Young produced 1(I1 n

I l __Adult mortality l

lLI 1

l3 lYoung produced lOl^lOlnlClOl/lC l

l Ault mortality t

{

t 1

L 4

jYoung produced L

j l

i4 la j

L

-j l

i4.

l Adult mortaty l

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n 13 l J Adult mortality L._

I-I l

l 6 jYoung produced l.

{~i _ 2 12 l 2 L

§IJi i

.8I__I_

I 6 I S

Young produced L

~

j

~

/

I Adult mortality L

I a

7 Young produced 1.

l TolYyoung produced l 3 1 l l1 l3 Z lS 3 Zl3 j3 Fial AduLt Mortality l

T k C I lT I c7 l

C"i 1

u l

T C

lll Concentration:

% Mortaliy :

I Mean Offspring/Female:

I 32.

% Reduction from Contxol: --1e t71 (A

Environmental Tcstine Solutions. LLC Pale 4 of 7 Species: Ceriodaphnia dubia Client: TVA -Se&%AA Date:

011-05 CONCENTRATION: 1Z%

Survival andReproducion Data

~~~~~~~elie nmer--

Day 2

3 4

5 6

I 1910 I

Young produced C

O O

0 -

I O Adult mortality L

L 2

Young produced C)

I l

hl

-I 1

I k>

Adult mortality l

J W

l

_-l_

3 Young produce l

,. L 3

Adult mortality S

Young produced l4 1

i

'4 l

O t6 tl8l

°l-Adult mortalit I

t

_ Young-poduced 12-.-

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Adult mortality U

L I

L L

7r z~~~z I~~I L 7 __I U.U 7

Young produced IoIS I

I'S I Fmdizit. mortalty j

Ll I U c~

I lIl_

Lci 6oa young produced

~

j 2.IL C L Ii 0

iZ Final Adult Mortality L___L l Concentration:

% Mortaity:

l Mean OffspringfFemale:

l3Z.1

% Reduction from Control:

t.

a CONCENTRATION: icd7Z Survival and Reproduction Data I

Rcplicat number I

_Dav l

It_

2 l

3 l

4 5

6 l

7 a

9 to rX lYun produd 7l1 I

CB T e7 I

e) l l

~Adult morlt

-lL_

Ll L

L L-lL 2

.lYoung produced In f1eyFO IO IOl<)1 0

Adult mortality j U- _

3 lYoun produced l_

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(

l O

L l

l

~Adult mortalitq l

-_l l

t 6

l e

}

§ l

4 Youngproduced S_

Li L

L Adultmortality l

t_

l Young produced 1 Q j

j3 j ijO 13 lJI I I Adult mortality l

LI_

L L

l l

c=

6 6

Young produced l

'Ii A

Adult mortality L'JIL I L.I LI1tW L

I L-II I

7 Young produced 1-7L UrL~CO Z3 I

-i1 TotalAyoung produced 37rtt yZ l

5 1

UI.. 35 11 l 3. lU.

Finl Adult Mortaty LG lQ l

(4 L

l l

l Concentration:

I%Monality:

I I Mean Offspring/Female:

13.S

% Reduction from Control:

- 25. a7.

EvrnetlTsigSltos LLC Environmental Testing Solutions, LLC Page 5 of 7

, t-r Pag 5 of 7

r.

-1 r

M.,

Species: Ceriodaphnia dubia Client: TrVMkSeGSr'A Date: tb-11-61S CONCENTRATION: I%

l e

Survival and Reoroduction Data Repiicate number Day 2

3 4

516 7

aT 9

10 I

l Young produced O

C Q

O o

Adult mortality C

. r 2

l Young produced o -

Adult mortality l

W L4 U

L...

L L

3 Young produced 3

C Adult mortality l

L-L l

l 4

Young produced l

4 j

1 L

-L 3-Adult mortality t

L l

l V

l.l 6

Young produced l

6 l

O 0

12-Adult mortality ILI_

l l

l 6

Young produced 0

0 l

12 1

I 1

=

IAutmortality

_1-_

7L Young produced t

I2 15OQ-Total young produced Z

zf l 33 l z"

FInal Adult Molrtality ILJ LI LCI Lj L

L T7 1 T

T

/1 lConcentralon::

l

%Mortality:

l Mean Offspring/Female:

Z-I

% Reduction from Control:

Environmental Testing Solutions, LLC Verification o Ceriodaphnia Reproduction Totals Control Day Re llcate number Total 2

3 l 4 l 6 7

3 9

30 O

O O

O0 O

O O

2 0

0 0

3 0

0 0

.4 3

4 3

3 3

4 4

4 3

4 35 5 9 0

0 0

0 10 0

33 9

9 48 0

30 1 32 0

10 0

0 0

50 7

18 6

32 34 33 36 12 34 12 16 143 Total 30 30 23 27 21 30 26 29 24 29 276 Day Replica't ou ber Total Day _

2

~3 4 S

7 tta 9

to0 To~

0 O

0 0

0 2

0 0

0 3

0 0

0 4

4 3

3 5

4 3

3 36 5

0 0

-0

-0 1

-0 0

1 1 30 13 10 45 9

I 9

10 0

113 0 0

60 14 14 12 16 14 17 17 13 15 I 18 ISO Total 27 29 24 30 28 30 33 23 33 31 293 22%

Day 3

Re Plate number I

3 4

5 6l 7 l t Tot l I

0 0

0 2

0 0

0 3

0 0

0 4

S 4

4 3

4 4

4 3

5 3

39 S

0 0

13 0 1 14_ G° t 0 0

47 I

33 12 0

0 0

3 3

63 1~~~~~~~

I 7

35 34 37 6

3 34 14 37 4

Total 31 3

34 29 37 283 283 30 32 23 0

43.9%

Day Repicat number Total

.2 3

4 S

61 7 3

9 10 1

l 0

a 0

0 0

01 0 0

0 0

0 2

0 0

0 01 0 0

00 0

3 0

0 0

4 4

3 4

6 4

4 3

4 40 5

I0 0

0 0a 32 0

0 32 301 47 6

3223312 10 3

3 00 0

72 7

1 915 1 17 17 16 713 2019 169 Total 3S-311 34 31 32 3S 2

2 33 3

72%

Day Re Icate number Total D

2 3 l 5

6 7

a 9

10 2

0 0

0 3

0 0

0 4

4 4

5 4

4 3

4 4

3 5

40 S

0 I0 0

0 134 31 12 0

30 47 6

14 110 12 1

0 0

0 30 0

11 63 7

1635 18115 13 37 11719 17 201 172 Total 34_l 29l 35 l 30 36 31 33 33 30 36 327.

100%

Day D

I pilet number T

I l

y l I l 2 3 l4 Sl5 6

7 l to 0 t

I 0

0 0

2 0

0 0

0

.0 0 0

0 0

3 0

0 0

4 5

4 4

4 13

.5 4

4 5

l4 42 5

1 0 _O_

0 0

13 1_

__0 13 itl 14 1 63 6

114 13 11 I 15 0

0 1 13 O.0 0 10 7

III

17. 17 14 191 19 18 20 15 17 174 Total 37 34 32 33 351 36_ 35 37 31 351 345 100% Intake Da

-tRepicate number Total 3

2 3

4 5

6 7

3 3

o0 I

0 o 0

o I 0

2 0

0 0

3 0

0 0

4 3 -..

4 3

4 4

3 4

4 33 5

10 11 0

0 0

1I 0

0 0

12 44 6

1 0 0

12 3

12 11 0

9 2

l0 66 7 3 14 4 18534 5

6 2

517 150 Total 23 29 3331_ 29 30 29 1 33 293

..I.

Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)

Species: Ceriodaphnia dubia Quality Control Verification of Data Entty, Calculations, and Statistical Analyses TVA Sequoyah Nuclear Plant, Nontreated alent:

Test dates:

June 17-24. 2003 Project number: 744 Reveiwed hr. _r-Concentration Replicate number Survival Average reproducton Coufiduet of Paral rducita r,_

(%)

1 2

3 4

5 6

7 8

9 10

(%)

(offspring/female)

Control 30 30 23 27 28 30 26 29 24 29 100 27.6 9.2 Not applicable 10.98%

27 29 24 30 28 30 33 28 31 31 100 29.1 8.6

.54 22%

31 30 34 29 37 2S 28 30 32 28 100 30.7 9.6

-11.2 43.9%

35 31 34 31 32 35 32 29 33 36 100 32.8 6.7

.188 72%

34 29 35 30 36 31 33 33 30 36 100 32.7 7.9

-18.5 100%

37 34 32 33 35 36 35 37 31 35 100 34.5 53

.250 100% Intake 28 29 33 31 29 30 29 27 29 33 100 29.8 6.7

-2.4 Outfl l 112:

Dunnett'i MSD value; PMSD:

Intake:

Dunnett'a MSD value:

PMSD:

,5aS 2.SIZ op-

'.1 1.772 6.4 MSD -

Minimum Significant Difference PMSD -

Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole efluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.9% from the control.

Lower PMSD bound determined by USEPA (10* percentile) 11 %.

Uppcr PMSD bound determined by USEPA (90 percentile) - 37%.

The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cerfodaphntlareproduction in chronic reference toxicant tests.

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Emuent Toxicity Applications Under The National Pollutant Discharge Elimination Program. EPA-33-RO0-003. US Environmental Protection Agency. Cincinnati, 01 I.

Environmental Testing Solutions, LLC Statistical Analyse Carlodaphala 3arrval ad R.prsdsetiou Tet-Repreductlea ut Dat k l17.2003 Test D CdFRCI.

sample 3wpuyshNuktpla&,4 Cht611 101 11Dac 24.200 Lab Mt T&En,. TatmgSAww Sm pTe TO C&DAch-p oMadi;xRpOt Sompla Data hoeL aDDNI

-A421.L0Z013)

Tea Speciac Cuiodapbnia dhbia Csc-f%

1 2

3 4

S a

7 i to D.Canul 30.000 30.000 23.000 27.000 211.000 30.000 26.00 29000 24000 29.000 10.9S 27.000 29.000 2000 30.0O 2S00 30.000 3D.00 2.O 31000 31.000 22 31.000 30.000 34.000 29.000 37e00 2.00o 2LOOO 30.000 32.000 2s.000 43 35.000 31.100 34.000 31.ooo 32000 35.000 3000 29.000 32.000 36000 72 34.000 29.000 3.o000 30.000 3000 31.000 33.000 33.000 30.000 36OO 100 37.000 34.000 32.000 33.000 35.000 366000 35.000 37.000 31.000 35.000 Trasater: Uatrasformed I-Tailed be6tewl-Coec-%

Mean I4.Mens Mum bi Mas CV%

N

"-Stat Critical MSD ame Na D-Contral 2.600 1.0000 27.600 23.000 30.000 9.229 to 31.33 1.0000 10.9 29.100 1.0543 29.100 24.000 33.000 1.641 10

-1.349 2.237 2.542 31.233 1.0000 22 30.700 1.1123 30.700 23.000 37.000 9.595 1o

-2.79 2.227 2.542 31.233 1.0000 43.9 32.300 1.1384 32.800 29.000 36.000 6710 10

.4.678 2.237

.2.542 31.233 1.0000 72 32.700 1.184 32.700 29.000 36.000 7.903 10

4.53 2.237 2.542 31.233 1.0000 too 34.500 1.2500 34.500 31.000 37.000 5.137 to

.6.20 2.287 2.542 31.233 1.0000 Ausiliary Teats Statistic Critical Skiw Krt KYkrngoro D Tat isdicates nimal disnitio (p > 0.01) 0.524606s2 1.035 0.00625967 0.350923 etes Test ndicates ual warunes (p 0.91) 1.502159i3 15.0863171 Hyyetk-l.

Test (1-tail. 0.05)

NOEC LOEC CtV TU MSDa MSDP "V5B MSE F-Preb df Dutett3Test 100 100 I

254175679 o09209264 66.6266667 6.17777778 3.2E.07

5. 54 Treatmes n D-Contrl Linear latepolatiew (200 Rlamples)

P at SD 9% CL Skew 1005

'2100 0Clo co SOIS

',100 1.0 ic~~~s X2-loo CAi IC40

'10D 0.7 10SO

'loo 0.3 0.5

a. 0.4 CO 0.3 w 0.2 E0.1 0.0.

1

.0.3

.0.4 0.0 20 40 60 so 100 120 Dse-Resposae Plot 35 30 25 U

i 20 G.

a t 5

I I ---.

i I

I-taM. 05 level of sknifiance I

a rM

.a iv zqp!_G6-17-OdafV.z

Environmental Testing Solutions, LLC Statistical Analyses Celodphn Surivral and RpMraducton TestReprisduction StartD

h.

I17.W2003 Tat 0 ClFCR Sauple 1D.

Duy:h Ncdlr Plant talm Ind Daim

24. 2003 Lab Et E

v.

Togsobaim SpleTypc DischonitCgRaxpt 5k"ple Dot:

Protocol CRON PA2102013)

Tat Species:

C~.ciodp is i dbia Cenc.%

1 2

3 4

5 7

t 9

U D.Control 30.000 30.000 23.000 r7.i00 23.000 30.000 2&000 29.000 24.000 29.000 100 21.00 29.000 33.000 31.00 29.000 30.000 29.000 27.000 29.000 33.000 Transform: Untransronemd 1-Tailed Conc-%

Mean N-Mean Mesn Min Max CV%

N t-Stat Critical MSD D.Controi 27.600 1.0000 27.600 23.000 30.000 9.229 10 tOo 29.800 1.0797 29.800 27.000 33.000

&674 10

-2.153 1.734 L.m Auxiliar Tests Statistic Critical Skew Kurt Shapio.-WiLlks Test indicates normal dalimbuton (p > 0.01) 0.95612454

.0.361

-0.33926213 4.54343952 F.Test indicates equal rianecs (p

0.47) 1.6404494 6.541572 Hypothesis Test (1-tail. 0.0S)'

MSDu MSDp MSB MSE F.Prnb dr lbicueduc t Tat icasnosignifwxndiffertnces i.7721716 0.06420938 24.2 5.22222222 0.04515683 1.33 Trrntsw a D-Control Dose-Response Plot i20

i. Is SL K:4 xqn 06-1 7-0.3daxfr

Environmental Testinz Solutions, LLC Page I of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas Client:

Facility:

NPDES #:

Project #:

6ThX'IA 04 kuue, kAhrr T K-Q0Z(VASO

- -- Iggb I

zpe Dilurion reparation information:

IComments:

Dilution PM (b$)

I lo.& Z2.k

" Z I

fI1 I

CA

) LOAS Effluentvolum (mL) 12FL16 L'+IO I,-,r I iqq1 IZOCn uV-1tnjreb r-QP z7 141-I Oiluentvolunc(mL)

I

IT

Iso I

twnI.

A t.lA Nrr Diluent vlume (mL) 11&0 o.t I tS6 177 I STQ I A t.vn A

tt AhfaT I vlmcL)(

20N Z C)O ZCC 2C:l

)j e

z I~S L

ce Test organism information:

Test information:

Organism age:

2 7.

Q.5 - hopes 0j0 Randomizing template:

'4le; Date and times organisms Ca._-l-6 i\\0 c0 lWcC) lncubator number:

3 were born between:

MbV Icbtrnme Organism source:

AC 6A1Q-Nb-Ito- 03 Artemia lot number o

Transfer bowl information:

pH Temperature -

C Total drying time:

Rho__

SO OZ4.

'C.

Date Time in: ti,-.T 153 Average transfer volume:

Date/ Time out: 06ob-I O B

Oven temperature:

Io3C Daily feeding and renewal information:

Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding renewal, or batch used used time time termination time

MAC, o

o W

s 1 o

_ _ _Ho^>ios1 o

2 ab o'0o Ho oI0 3o

-2 0____

rfl6-1-103 05o.1 i.OtIU 3 CJ0 7D 83 (Ds, 0 1'55 ItA?.

C I

b-

-00

.0 1 I. 07 4

6Z CA A

C, b h WO c-11-6s 05C(2 I. O(.o 0 s

N-n-o3 Ml3-zO 15 is IIO-ZI aC3a2I.0 0 0-'7 6

aM o3 o

2.

O OCL,21 03 ol0Lo.I.o.67 7

16

_e I

_i Control! inormation:

A ipta critena Summarv of test endpoints:

l

% Mortality:

O.

S20%

7-day LCs5

>100 I

Average weight per initial larvae:

I O.&I1 so_

NOEC I

I Average weight per surviving larvae:

0.8110 2 0.25 mgflarve lLOEC

> oo 2.

ChV "A

11q i l 1 C -

i

) / 0 0 2-.

C.

vironmental Testing Solutions, LLC Page 2 of 6 vironmental Testin! Solutions, LLC Page2of6 Species: Pmephalespromdas Client: 'TIA - Se6k51 AA 0- -TR*Wr

Date: 0'6-r7-0A Survival and Growth Data Day CONTROL JO 22%

A B

C D

E F

G H

I J

K L

0b

/0

/

/0/0

/

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6 Ir ErA Comments:

I~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

vironmental Testing Solutions, LLC Pag 3 of 6

,ironmental Testing Solutions, LLC Paae3or6 i .:

o' t-..

1."

t:

.iE t '

a.'9, Species: Pimephales promelas Client: T7A - SezQc4AA Date: -Al17-43

-A Survival and Growth Data -

Day M

N 0

P Q

R S

T U

V W

X

/0

/

l

/ 0

/o) J /01 1

1) 1 I/O

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N 4 6,

4' 5\\° 6

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ronmental Testing Solutions, LLC P..

0-A tv:

6G '

A Species: Pimephiespromelas Client: fT-A i5rIAA4 Date: _L-I1-OQ3 j %)-rWA-%V Survival and Growth Data Day I0%

I WrKE Y

Z AA BB o

IC) l 0 O 10

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o 16 A-PanL weight (mg) f -s

- Pan + Larrae weight (mg) a Larvaeweight(mg)-A-B aNX OA Otx.

do' i

-alculauions and data reviewed.

Conments:

(

Environmental Testing Solutions, LLC Chronic Whole Emuent Toxicity Test (EPA-821-R-02-013, Method I000.0)

Species: Pinephalespronelas i

Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Soquoych Nuclear Plant. UV Treated Client:

Test dales:

Project number.

June 17-24. 2003 744 Revelawed by IJ Caaeeairalhn (%)

RepUcak Inal a*dumwer Final numshr u

A -Pawei B*Pan+Larvae Lam. weigh (sa)

Iglt I

la abe Measuvival bea u1eh (of Ped rd_

bryan (0ag)

Tight (MO A.1 4.iarVas(Mg)

(S vsf (CA) n A

10 10 15.157 22.380 7.223 0.7223 coasro~

B 10 10 15.09S 22.780 7.635 0.7685 100011 07Ntap~al Coltrol C

10 10 15.079 23.3B0 8302 0.3302 10.0 0.ti l IQ7 Not plikabte D

IQ 10 15.031 24.260 9.229 0.9229 E

10 10 14.685 23.080 8.395 08t395 30.98%

F 10 10 1 5.005 23-310 3.305 0.1305 97.5 0.8813 63

-8.7 0

1 0 10 14.842 23.960 9.118 0.9113

.______________ II I 0 9

14.655 24.090 9.435 0O.9435 I

10 10 14.788 24.530 9.742 0.9742 J

10 10 14.920 23.120 8.300 0.800 22%

J 10 9

14.550 22.920 3.370 0.8370 97.S Q9084 6.5

.12.0 I.

10 0

15.00 24.430 9.422 0.9422 M

I0 10 14.t46 23.070 t.224.

0.3224 43.9% ~

N I10 10 14.690 13.220 3.530 0.8530 43.9%

0 10 10 15.013 24.460 9.447 0.9447 100.0 0.8796 61

-8.5 P

30 10 14.959 23.940 t.9t1 0.91 Q

10 10 15.171 24.260 9.089 0.9039 72%

R 1 0 10 15.263 25.710 10.447 1.044797S0935.

2.

9 I 0 10 14.975 25.060 10.05 1.0035 9t5 Q9S32 59

-21.2 T

0 9

15.055 24.760 9.705 0.9705 U

10 10 14.973 24.990 10.017 1.0017 100%

V 10 10 15.107 24.490 9.383 0.9333 100.

0.8928 12.2

-1Q.

W 10 10 15.004 23.360 3.356 0.3356 X

10 10 15.096 22.550 7.454 0.7454 V

10 10 14.t36 23.440 8.604 0.8604 100% nel.

Z 10 10 15.060 24.220 9.160 0.9244 10.0 0.9373 0.5

-156 AA 10 10 14.766 24.010 9.244 0.9244 B

10 t0 14.936 25.470 10.434 1-.0434 Outran lol:

Dunnetlr MSD value:

PMSD:

Intel.e:

Dunnatls MSD value:

PMSD:

0.1254 15.5 0.1142 14.1 MSD -

Minimum Significant Difference PMSD Percent Minimum Significant Difference PMSD is a measure of teat precision. The PMSD is dhe minimum percent differcme between the COntrol end tetment that can be declared statistically sigmficmnt in a whole eluent loxicity esL On average. a sniicaut difference ocusis hArEnvirenmental Testing Solutiona. LLC chronic loxicity teats when a toxicant reduces Pimephales growth by 16.t% fwm the control (determined through Lower PMSD bound determined by USEPA (I Oth percentile) 9.4n Upper PMSD bound determined by USEPA (90th percentile) - 35%

The lower and upper bounds were calculated by the USEPA using 205 tats conducted fiem 19 laboratories for Pinusehles growth In chronic reference toxicant teat.

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Efluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-133-R410-003. US Environmental Protection Agency. Cincinnati, Oll.

Environmental Testing Solutions, LLC Statistical Analyses I ik Ge-t sSwvu Toat-7 Day Gwtr Dwte 617M3 Teat I PpFRZ Sample D:

Sequah Nucear Plat 0O4 l01 ld Data:

1141W0 Lob Mt 134w. Tooft Soludwo San& Type:

D U

S Z-01lbarr Idrling RPal Sample Deta ftncot CHRO IC.(EPA4214-ZI2 0

Ta Spede PP-Pkwptab ptada Csa-%

I 2

3 4

Clnma 0.73 0.71615 (Lc 0.929 10.93 0.395

.3203 0.91s8 0.9403 22 03742 0.33 1.370 0.942 43.9 GWS=A 0t3o 0.441 0.I3 72 0903 L0447

.03 00 loo o 17 0

0.6 0.74S4 Teandws UItmns rm

-Tan" CeaC-%

moan N-es man MIR Max CV%

N 11-1tat Cutical MOID M~ean N-Mea D-.Cont 0.110 I.OO0 0.8150 0.9229 10.694 4

0.927 I.OXO 10.9 oLW1 L.OW 0.3513 0.W0 0.943$

4.253 4

-1.232

-.410 0.1254 0.392 1.0000 22 0.9084 1.1201 0.9034 0.3370 0.9742 4.779 4

-1.J72 2.410 0.124 0.8927 1.0000 43.9 0.3796 1.044 0.36 0.224 0.9447 4077 4

-1.318 2*10 0.1254 0.3927 1.0000 72 0.92 1.2123 0.92 0.9039 1.0447 5.903 4

.309 2.410 0.1254 0.8927 I.OXO 100 0.39X 1.1003 0.3923 0.7454 1.0017 12.221 4

-1.372 2.410 0.1254 0.927 1.0000 Amailary Tests Stattic Critical Skew Kurt

Shsp, sWihsTea sumates onsLdistrbuum(P>0.0 1 0.97625077 0.884

.0.2164391 43347123 Bardstr Test ndiles qual r

s (p 0.7) 2.47064233 153.063171 Hypock-if Teat (t-talL.35)

NOCC LOIC CItV TU MSD M

MSDp "33 MSE FrProb 4r DunaswTest 100

O1 I

0.1233815 0.154605S7 0.01232393 0.00341331 0.09033341

3. 13 Trments a

-CtIut Uses Iterpelatio (00 Ramplse)

Peint SD n

Cp) skew IC503

>100 ICIO

.'500 IC13 300 1.0 IC20 100 0.9 IC25

'(00 0.8 IC40 100 0.7 I50

'100 0.3 a 0.4 0.3 K 02 0.0

.t.

0 20 40 s0 s0 tOO 120 Doss %

-ese-Respease Pt 1.2 1Wail. 0.0 Wl d significance I

sqn64717 03dawalr

Environmental Testing Solutions, LLC Statistical Analyses Larval Cis Grwth and Surval Tut-7 Day GCwth SMt"ic 4117/03 Tst lD:

PPFR=

SAWLCOD Seuoyah NudarPlan; ktiakc End D=

624/03 LaD g M.

Testin Solutions Samitrpk c

CM oksch tuiReort Saa DcMC Psotocol:

IRWN=-PiAl-R24.13)

Tat Specis:

PP-Phphdc mp zs Co mits:

UV4rested COac.%

1 2

3 4

D.Coi=

07223 0.76S5 O.S30t Q9229 100 0.604 0.9160 O.944

.0434 Transtoem: Untransformed t.Tailed Canc.%

Mean N.Mea Mean Min Mal CV%

N t-Stat Critical MSD D.Conual OSIIO 1.0000 0.110 0.7223 O9229 10.694 4

to 0.9373 1.1558 0.9373 03604 1.04t4

-.463 4

-.150 1.943 0.1142 AulHary Tests Statistic Critical Skew Kurt SMpim-WilWs Test indicates nonmi distnbuion (p >0.01)

O3905384 0.749 0.64399405.0.39201934 F-Test indicates equal winces (p 0389) 1.19533205 47.4683456 Hypothesis Test (1-tail, 0.05)

MSDu MSDp MSB MSE F-Prob dr Hocoscedastic Test indicaes no sinifi differnces 0L11419269 0.1400913 0.03191601 0.00690685 007514437 1.6 Treatrrns w D-Control Dose-Response Plot 1.2 1

= 0.3

_9 0.6 a

Q2 0.4.

0.2 1t4al. 0.05 level of signifkance 1 i I

&qn_06-7-03dax.xI

Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)

Species: Pimephalespromelas i

Dally Chemical Analyses Client:

Sequoyah Nuclear Test dates:

June 17 -24. 2003 Plant Non-Treated Project number:

744 Reviewed br Day S l

Da 6 I

I Fsl I nltIl I Flnel I Y

I Concentration Parameter DayO I

Initial I Final I Initial I

4.

I

.T1 LaTin

.TXlr ft~

RI

-7 IV)I 771 tNU)I I OV-.

I I

Ul I I

)O tinLft-I-A.

Control wnuwivsnv t>§wutuv}

klkallnity (mgtL CaCQ)

I fardoess (mg/L CaCq) remernture CC)

H (SI) 7.69 10 (mfA/L1 10s%

onductivity ftmh sm) i 301

~ 29_O~

2_ RJM 2S.'

?A 71 t

1,_

__A

_e

_Z ILt -yl A,

I r Si 7.71 7.54 7.v7

+

I

-l---I---+

I (multi 7.

7.3 v

22%

. _L

-- -q q 0 t

'^ndurHvliv tmhae/rml

!~~~~~~~~~......

&mneraure tl I

25.5 24.71 25.31 24.61 25.

24.

25.21 24.51 25.21 24.71 25.31 24.31

.25.(

24.7

-.-- - -1 I (SU) 7.6 7.541 7.661 7.671 7.7 7.6 7.641 7.531 7.6S1 7.431 7.711 7.501 7.6 7.51 (mg/)

1 7.91

7. 21 7.8l 7.41 7.ST

7.

9 9

7.41 7.6 671 7.61

?.4 7.71 7.

1

.- b

^n i

A--

A 43.9%

-o

-1dtlv fumhoatteml 24 243 24 242 746

.j q

f1 N1-I Temnerature CC)

I 25.5 24.71 25.31 24.61 25.3 24..

25.11 24.51 25.2 71 I

I--I-C-I-nH~ (SMf 7.6:

7.541 7.601 7.631 7.6 7.6, 7.561 7.541 7.61 SI 72%

DO (mg/L)

7.

7.4]

?

7.81 7 S 7.5k 7.7 6.71 w

_t

_s

^

An!

Conductivity (mhoscm) 215 Temnerture C) pH (SU)

Conductivity (;mhos/cm) 100%

Alkalinity (mg/L C*CQ)

Hardness (ml/L CCO)

I Total Residual Chlorine (m

. l

____I.

Temperature CC)

S ~~~~~204 204

~

20? ~

I" 204 3.246 _

25.3

24.

25.21 24.5 25.3 24.7

25.

243 24.7 24.

7 1.62 7.62 7.6 7.47 7.49 7.55 7.421 7.66

.43 7.60 7.45 7.4 7.9 7.7 3.0 7.6 7.9 6.9 7.6 3

7.

166 1

167 161 6

164 53.1

~~~~~~~~~~5.1 626._

62.6 _

24.6 25.3 24.5 25.2 24.6 25.4 24.7 25.4 24.3 24.9 24.7 S 7.62

~7.671 7.641 7.471 1.9 7.541 7.411 7.531 7.431 7.51 7.45 i

?.4

?~.91 7.71 S.O 7..91 6,S1 7t8 s.7.3 7.7S s

~~~~~166 1613 11.1#_

5S.I~~~~~~~~~~~5.

62.6

~~~~~~~~~~62.6^

Bl 24.61 25.31 24.51 2S.2 46 2.1[

24.?l 25.4 23 24.1 247 ntH (SUfl 4-DO (ma/L)

0.

Conductivity (tmhos/cm) 100% Intake Alkalinity (mg/L CsCQ)

Hardness (mglL CaCO)

Total Residual Chlorine (m/L1 Temperature eC)

Environmental Testins Solutions. LLC Pagte of 6`

Environmental Testing Solutions. LLC Paee S of

.. ~,,

,-., j

,_I... 1.....

Species: Pimephalespromelas Client:

JA-SaeNS4^

Date:

06-M-AV, rJotrnAT Daily Chemisty:

Y I.

Dav I

I

-. 4 Concentration Parameter 5

I 1

I 2

1

CONTROL NAS DH (S.U.)

L.W.O I -I ki, I

r-z_-_-w L

l l

e X

F DO (mglL)

Conductivity

&Oo (pndlos/cm).

1 Alkalinity (mg CaCO31L)

C0 Hardness (mg CaCOL3)

I Sz.

Temperature (C)

Z55

4.

.9 pH (S.U.)

10.q9%

DO (mgL) r_

P~~~

Lonaucliviy

.Zqo IMM I

. Temperature (C)

Z5.5 1

ZS.3 l_ Z-1

'Z 'S*3 pH (S.U.)

C

.5 Z.

i 2fo 3 2

.o 2

Z'i.0 DO(mgL)16 Conductivity a

(gmhoskm)

__2_4___

Temperature (C)

ZS.5 2&.

Z53 Z I. O 2S. 3 Z 4 pH (S.U.)

-Lbl4.. o.G '1

.O ALh a DO (mg/L) 2 4Z. e h

J Conductivity (unhos/em)

Z 4

pH (S.U.)

.UZ

(

t)

[s03

.0 DO (mg/L) 2a 7to

~Conductivity 5ZO 2Q3 Temperature (C)

ZS.

l 24.

233 2-.,.

2Z pH (S.U.)

4.551 l.tX

}(1 640

.(oZ to104

.00'6 Conductivity (vmhos/cm)

Alkalinity (mg CaCO 3/L)

Hardness el (mg CaCO_/L)

TR chlorine (mglL).lO.2 Temperaturc (C)

Z25 2q 1, b S3 pH (S.U.)

.55

),LIIO SLfflo

,(0 2

3.IX Fil DO (mg/)

Iq C

5tl>

16r Conhductivit l9 Ei5 y

/81774Z (Alkaliity 53 l\\dB

.Hardness U 0l\\_kE (my" CaC03/L)(Sb'_ Si TR chlorinc d.ID>bO.1De

.Temperature (°C3 Zm.

Zo I Tnitial I

Final I Tnitial I

Final I

Initial II Final I

IL

1

.1

Environmental Testing Solutions, LLC Page 6 of 6 Environmental Testing Solutions, LLC Page 6 of 6 Species: Pimephalespromdas Client: -Ar

-Q.

' JairrfQWn Date:

M&a.11-03 a1 a

Day

-e*,t#!.

Iy Concen-tration Parameter I

CONTROL pH (S.U.)

DO (mg/L)

Conductivity (inmhos/cm)

Alkalinity (mg CaCO3/L)

Hardness (mg CaCO,/L)

Temperature (C) pH (S.U.)

DO (mg/L)

I0.&9.6 Conductivity (gmhos/cm)

Temperature ('C) 3 Z-4

'1Q 30Z 1

Z?3~~~V1

_351

.30 _2

_. _z 17 7 8cr_

29(

1.1Z1 48 Z_1_

2q3 1

l

-7.5.Z K

Z.

I s

F off (S.U.)

, \\ _

DO (mg/L) 2V2.

Conductivity (Imhoscm)

Temperature (C) r

\\

=

pH (S.U.)

I 4.S3 1 -7io q3.qi DO (mg/L)

.q -

q 11 :1 I

1:L.(O Conductivity IZq Z

,(imhoslcm)

Temperature(C)

OH (S.U.)

-. A 2 7.

DO (mglL)

-. e Conductivity I

4

(;imhos/cm) 2

.M zO0 Temperature (°C) 25,

5 I

zg'.5 9

y ----.-

q

pH (S.U.)

-. q3 I

Iti iI I.rO I

I w-DO (nig/L) to.9L II Conductivity I (o S (gmhoslcm)

Alkalinity (mg CaCOi/L)

Hariness

\\

TR~~~~

hlrn (gLY 1S 4.1

-1 0 g 1

.1

.Io1

. I Temperature(C)

I I

I 25.

I zti. A I

I 74 I

r I

=

=

pH (S.U.)

I I.

41 I

.d.

4

I I

n l *--

DO (ma/L)

I I

-6a I

/0y2 l4A0k.

Ie Conductivity I q (pamhos/cm)

I J

Alkalinity tma r'rCi.n i I/(0 v

Hardncss (mg CaCO3/L)

I 3

TR chlorine (mg/L)

Temperature (MC) 25.z I

Ill.1 I

I

9.

Initial I

Fnal I

A.._.

Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)

Species: Ceriodaphnia dubia Daily Chemical Analyses Sequoyah Nuclear Plant Non-Treated Client:

Test dates:

June 17 - 24, 2003 Project number: 744 Reviewed by:

, p U

I Concentration I1arameter Day 0 Intiall I Final I

.5 1

I

-t I

I%

7 fl 7 '71 pn4 tans i

eu I

I.

DO (mYJL)

Conductivity (umhostcm)

I Control Alkalinity (mc/L CaCQ)

I-Hardness (mgIL CaCQ%)

Temperature CC 24.5 24.4 25.2 24.4 25.4 24.5

_25.

24.4 25.1 24.3 24.6 24.5 24.7 245 nH (SU) 7.69 7.73 7.69 7.73 7.75 7.88 7.72 7.95 7.72 7.93 7.761 7.81 7.67 797 DO (mWL) 7.6 7.6 7.8 7.6 7.8 8.0 7.8 8.0 7.5 7.8 7.61 7.9 7.7 83 10.98%

Conductivity (umhos/cm) 3(01 i

29C i 287

_2SC 29t 276 TemperatureCC) 24.5 24.4 25.3 24.4 25.4 24.5 25.6 24.4 25.1 24.3 24.6 245 24.7 245 pH (SU) 7.70 7.73 7.67 7.73 7.75 7.88 7.71 7.94 7.72 7.90 7.74 781 767 796 DO (mg/L) 7.81 7.6 7.8 7.6 7.8 80 7.9 8.0

7.

9 7

7.9 7.7 8

22%

Conductivity (jmhos/cm 284 279 27 273 282 265 273 Temperature CC) 24.5 24.4 25.2 24.4 25.4 245 25.6 24.4 25.1 24.3 246 245 24.7 245 pH (SU) 7.66 7.70 7.66 7.72 7.70 789 7.64 7.93 7.68 7.89 7.71 782 7.6 797 DO (m/L) 7.9 7,7 7.8 7.6 7.8 80 7.9 7.9 7.6 7.9 76 80 77 Conductivity (mhos/cm) 24 243 247 24 246 243 20 Temperature CC) 24.5 24.4 25.2 24.4 25.4 245

25.

24.4 25.1 24.3 246 245 24.7 245 pi (SU) 7.62 7.67 7.60 7.70 7.69 74 7.56 7.87 7.61 7.87 7.69 781 760 793 DO (mg/I.)

7.81 7.7 7.8 7.6 79 80 7.8 7.9 7.7 7.9 7.6 80 7

8.5 Conductivity (Itmhoslem) 21 206 204 204 207 199 204 Temperature CC) 24.5 4.4 25.2 24.4 25.4 24.5 256 24.4 25.1 24.31 24.6 24.5 24.7 24.5 pH (SU) 7.57 7.65 7.52 7.68 7.62 7.81 7.43 7.86 7.55 7.80 7.66 7.79 7.60 7.91 DO (m/L7j 7.9 7.9 8.0 8.0 78 7.9 7 8 7.6 8.0 7.8 8.4 Conductivity (umhos/cm) 16 167 167 165 16741 166 100%

Alkalinity (mg1L CaCQ) 57.1 58.1 57.10 Hardness (mg/L CaCQ) 62.6

66.

_62.6 Total Residual Chlorine (m/L) 4H.10i

<0.

O 10_

Temperature CC) 24.5 24.4 25.2 24.4 25.4 24.5 25.6 244 25.1 24.3 24.6 24.5 24.7 24.5 pH (SU) 7.59 7.66 7.56 7.68 7.67 7.84 7.47 7.84 7.54 7.82 7.53 7.741 7.50 7.78 DO (mg/L) 8.01 7.8 7.9 7.6 7.9 8.0 8.0 7.9 7.9 7.8 7.8 8.0 7.8 83 Conductivit (limhos/cm) 168 6

163 100% Intake Alkalinity (mg/L CaCq) 58.1 5S 51_

Hardness (mg/L CaCq) 64.6 62.

TotalResidual Chlorine (mAL) 0.10

<0.101 c.10 Temperature CC) 24.51 24.41 25.21 24.41 25.41 24.51 25.61 24.4 25.11 24.31 24.61

24.5 24.7 24.5

]Environmental Testing Solutions, LLC Page 6 of 7 Enirnena

.etn Souios LLae6o Species: Criodapnia dabia Client: -NA-S iAi Date: wI-t1 -

r. Daily Chemsy Concentration Parameter 2:lCONTROL pl (.U)

O 0 ~DO (gSL)

>7 Conductivit_

^

~~~Alkalinity f

~Hardness (mg CMCzL L.

Temperature (C) 2;t)

Z pH (S.U.)

Slq DO (gL I. Ao 21*Conductivity 2l (gmhos/cm) 1 Temperatu re (C) 2 l5 2

pH (S.U.)

-4Q_

D(gL)

Z2I/a Conductivity (pinhos/cm)

ZL

~~~Temperature (C) 2+.:5 2-

~~pH (S.U.)

.07 xl3

.§ <:

Conductivity 4

(umhos/cm)

Z a

Temperature(°C)

Zq 5 tS pH (S.U.--_

2 12° Conductivity ZtI (gmhos/cm)

Temperature C) 24.5 pR (S.U.)

+

i.iX DO (mglL)

>.4 Conductivity I

q (lmhoslc=r) t A00g (Amg CaCQ31t) 5 Hardness f.e (mg C 03/L) iE TR chlorine (mw1)

L.D.

i Tcmiperature (°)

2t..5 rt

~~~pH (S.U.)

.5 E.

~DO (n

tO

~~~~Conductivity

}

/X);

(pimho m)I 1

F

~~~~~Alkalinity

/^J~~te (mg CCO3) 58I K

~~~~Hardness w

S

~~~(mg CnCO,/L.)

X.

1*~~~T chlorine X.

~~~~(mgtL) ix _

~~~Temperatur (C) 7,4.5 2

Day Day 2'J 1 21.

i'4WA.

4 I. 1%

,.::6 AO M

M 14-3

.10 L22-1.1

=1 ME q-q I

n

{

R da'?

F M M

4-r A&-

1 (a W

IJA 1-496

-1:

.6

-J-6 Z814 14 2 1q, zero 2S-7 Z,;.(A

-:Mew

-1710 7MO

1. (gQ

-+.U9

-4. e 1a, a3 7c" 2oq 2S. 7

-7.-3z

-4.(07 b-D rO V4 4-.q L L UP FM C41t:1 24.5 I

I 25.'L-1 I If 1 C4 Initial I

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IEnvironmental Testine Solutions. LLC Paxe 7 f 7 Environmental Testinf SoIution. LLC PaRe 7 of 7 Species: Ceriodaphnia dabia Client: IrVA -

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..Concea-lLntio-Day

-a Parameter 3

I 4

I S

I Lr.^s

_e I

S Iv~

P6ROL 4:'

PH (SM.)

DO (mg/L)

G.o L

Conductivity' (gmhos/cm) 311 1

Alkalinity (mg CaCO3/L)

Hardness (mg CaCO3/L)

Temperature (C) 2S. So Z4.1Y

r pH (S.U.)

=Wiz E DO mA) 8 0

.5 lo,5%

Ccnductivity Temperature (C) z w lz pH (S.U.)

-.51lIo DO (mL) 22.

Conductivity I 2q3 1C

(,imhoslcm) 2e2 Temperature (C)

ZS. to Zt.t[

9 t~~~

~~

I*

OH (S.U.)

1.014 9

II. -*------

--=------ DO (mg/L)

._t

~,,

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(,umhos/cm) 24iZ vr

~

Temperature (C)

.C

.1~~~~~~~~;

OL-.

l e

10 mln M5

-Z27 LM 22v1

_ 7.;

Ie nq-q 7.1 M

Zo O

5

44.

z1.

5 4.1 t.

I W 0

\\

M m

V t,1 a 4

OH (S.U.)

4

I

4 11LJL1..

DO (mg/L) 121 Conductivity Conductivity 2'

5l (pathos/cm)

C 1 7-0 ZC r Temperature (°C)

ZS.1 pH (S.U.)

DO (mg/L)

Conductivity

.~~1(mhoslcr)I o 1 o Alkalinity (mg CaCO3IL)

Hardness (mg CaCO31L)

TR Chlorine (mgQ) e.

Temperature C)

Zq M 1

I pH (S.U.)

4.'4 -t I I

I

_1 -+.-l I

DO (mg/L)

I t5.c I

14T7ice-Conductivity

'LI1o

( imhos/cm)

I (A to 3

Alkalinity I

(mg CaCO3lL)

S Hardness (mg CaCO3IL)

TFR chlorine (mglL)

_.6.

to

\\

I=

\\

iNUUUM 1(4 M

16

\\

of

\\

o

=E s

A.

\\

MM-It w=M%1 L

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Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)

Species: Pmephalespromelas D

Daily Chemical Analyses Client:

Sequoyah Nuclear Test dates:

June 17 - 24, 2003 Project number. 744 Plant UV-Treated Reviewed by: CAI-I.

I Concentration IParameter Day 0 1

Initial I Final I 4-I H (SU) 7.691 7.531 DO (mff/Ll Control Conductivity (pmhos/cm) 3 Alkalinity (mgtL CaCCJ)

Hardness (mg/L CaCQ)

Temperature CC)

.9..

I

H (SU) 3:_

:1-

=

4-_

10 (me/L) 10.98%

2onductlivty (Rmhoslcml t

...1 -

remperature C) 25.41 24.8 24.

24.31 25.11 24.6

.4-

-I

-9

-- -4

-4

- H (SU) 7.7 7.4, I. I

-'.4 1 7I.

'-4 t!i

.4. --

4---

=4-

.L -4-..-

D0 (me/L) 7.

7.;

7.

7AA'I 81 7

22%

'onductivity (uamhos/cm) i 28 26

_Z6 i

2t2O.

-- 273i*

274 Temperature eCl 25.4 24.81 25.3 24.6 25.3 24.7 25.

24.81 25.j 24.~

24.i 24.31 25SZ 24.

25.'

24.81 25.4

24.

24.4 24.:.3 Er l

-X rh1 7

Acl 7

Cs 7

AS 7

7>

7 sC) 7 s11 7

sol 7

7t1

;1

-'-l

§ Is taui

1. 1 1.A 1.1!

1.0 I..

1.43 1.1

,.3 s t I

'.4.

I.,

-'.4 xi 7-fil

'-4 0 (me/Li 7.9 7.64~~~~~~~~~~~~~~~

7.7 7~~~.5 1

-fz j7 4

7.7 7.8 7.41 6.9,

7.

~~~~~~~~~~~~~7.4 8.01 7.41 BO~~~ ~

-mL 74

.E

9 1

-i

,.i.

43.9%

1 i

U Temnerature C) 25.4 24.81 25.3 24.

25.:

24.

25.71 24.1 1 nH 1t;11 I

I.

I I I

I 11

' 1.

-1...

I I 72%

_I I

I.

... I

.I I

I I J 1.LI I

DO (mdLl I

7 7.1 7.61 7.!

7.

7.'

7.7 7 71 6.

.onductivity (mhos/cm) 1 216 200j..

2001 2031____

Temperature MCi

=.,

nl IR lh In

{ouz

_~~~~~~~~~~~

In (me/L) t BM B

l

'anductivitv (Simhae/cmi 100%

Ikalinity (mg/L CaCQ)

I ardness (mg/L CaCQ) otal Residual Chlorine (mg/L)-

emnperature CI I

7.64 7.6

.51 77 7.58 7

7.5 7.661 7.46 7.741 7.47 7.64 7.49 7.6 7.7 7.4 7.9 6.8 7.6 7.5 7.9

¢ 7.8 24 25.3 24.7 25.7 24.8 25.4 24.7 24.6 24.3 24.8 7.63 7.67 7.57 7.59 7.54 7.61 7.4 7.69 7.36 7.54 7.3 7.6 7.5

-7.!

7.4 7.9 6.9 7.7 7.1 7.8 7.7 162 165 66 164 161 24.6 25.3

-24.7 25.7 24.8 25.4 24.7 24.6 24.3 25.1 24.61 I (SUl _

_~I D (me/Ll I-onductivltv (umholemi 100% Intake Alkalinity (mg/L CaCQ)

Hardness (mg/L CaCQ)

_ #. _.~~~~~--

r-&.1 Reies hi.rlne m.

I.-..

a.

..~-

1'emnerature tC) -~ or-.-._.

_ ^ _

__ _ x

z I.

ai Ite-I I

I I

i I

is Environmental Testing Solutions. LLC Faize 5 of 6; Environmental Testine Solutions. LLC Pace S of i Species: Pimephakespromelas Client:

TV A - Set'aQ0A A Date: OG-mo-3 Daiy Chemistry:

r Concentration Dav I

Parameter a

I I

I 2

I.

CONTROL pH (S.U)

DO (mg/L)

_1.

Conductivity S A (mhosrn)

A2laliniq X

(mg CaCO3/L) 4 C6 Hardness (mg CaC0311.)

4e

-- 1 -4.(Cl 1 -11 4-z 1

4.(o

-4. (1-9 Zee 26T Temperature (0C) 7Z.'5 I z9.r I

.9 9* --

pH (S.U.)

-DO (mglL)

Conductivity (limhos/cm) 3M

_1-5Sh.

I

-_ 25-3 Temoerature (C) e.1%

L I

pH (S.U.)

S.

9

9 1 --

I

*1*

9

-II-22'Z DO (mg/L)

'.-*wI Conductivity.

I_ z 4 z,

N Y1 (I (gmhos/cm)

_9 MM II 1AM=

Temperature (°C) zS.t4 Z4. 6 peS.U.)

Z3 o

4 Conductivity 2AS (gmrhos/cm)

Ztt Temperatur (C)

ZSW Z

3 V9.L Z 4.

pH (S.U.)

tt 2

-455 j J 10.tt DO (mgfL)

.1,

,5_

Conductivity 2 00 (pmhos/cm)

Z Temperature (C)

ZS.t 9*

Z5 3 2.3 2.

pH (S.U.)

-4.5-74 wZ

.5 DO (mg/L) 5' Conductwvity.C

- lbO7 (jimhos/m)

-+

RO B 31 Alkalinity (m CaCO3/L)

Hardness (mg CaCO9/L7 TR chlorine Temperaturc (C) z 2t

-3 ZM.

__Z4._

pH (S.U.)

110

-St1

-45 l

,'1 5

/,Bob.hdmucLi (n 00 15 Alkalinity IJ?1'iK&

(mg CaCO3/L.)

Hardness C_

(mgCCO 1)

TR chloAtne (

2 Telp rature (C)

Z6 Z.

I 3

l(

23 Z*

i I

I I

lnitial I

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Initinl I

Final I

Initial I

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I U...

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Environmental Testing Solutions, LLC Page 6d6f 6.

Species: Pimephaes promelas Client VA-ecNAA 4~- T~tAMM Date:

f-l-

- I T~~~~

ConcenZ-

.tration Day Parameter 3

I 4

CONTROL pH1 (S.U.)

.e Do ImgL Conductivity

Soo HS (wnhos/cm)-

Hardness-(mg CaCO3IL)

Temperature ("C) pH(S.U.)DO (mg/L)~-

Conductivity zq, (gmhos/cm)

_________Temperature

("C)

I 5

I.- i

-1 1111116,-A 5~ 2S.

z2

-=- 9-1 1 Iz I

I I

i oH (S.U.)

I.-

DO (mn/U 22%

- lb F?

Conductivity 281 4

Pu" TeMoerature M")

V.4.Io p(SU.

9 3.to tt

-4.4

,43.Vq"Conductivityzq (pmrhos/cm) q fz2c

______Temperature

(")

2 Z'i.1 zJ pH (S.U.)

-7 Conductivity I-I (jimhos/cm)

___it)

Zoq

______Temperature

("C) 32

.L

%.V

4. fo MI2qq R-0 3

IS..

z4.1' F

3 Zs.0 z~.c

-q I

14~~~~~~~~~~~

3l vze 2

OKH(S.U.)

1IL5o

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1 I.

I I

DO (mgIL)

Conductivity Alkalinity 1

V (mg CaC~~~~~i~~l5

)

I

- (" -1 in 19 IW-4 0M -

N I

Hardness-fma CnCO-IL)

\\

MEMO I (P03 I

I 1

I TR Chlorinc (mg/L) I J Temperature (")

I I

I

-- V-I 10 %

Conductivity (lnnhos/cm)

/AI~f K-C Alkalinity (mg CaCOA/)

Hardness (mg CaCO3/L)

TR chlorine (mgIL)

_________Temperature (C)

I LV-

-4. 09

-qsq I

I I to4 an

\\

gm M

14 24

-es I J'N-ka Tniti~I II finni I

Tnitiat I

Final I

Initial I

Final I Initial I

Final

__________-----____________________a-I

Page I L-of -

L Environmental Testing Solutions. LLC Environmental Testin2 Solutions, LLC Page

of Z.

q.

Alkalinity (EPA Method 310.1)

Matrix Water, MDL - 1.0 mg CaCO3L Titrate samples to pH = 4.50 S.U.

Anal st 7Et Date analyzed

o.

,n S r>lan aor ntrd dut~etermination:

pH of Normality Normality (N) of EzSO4 pH Factor or Multiplier Deliolzed Titrant check Begin End Total

-(5 ml NazCO s 0.I0S)E

= (Ns 50000)1 100 ml sample water reference standard Rd ml ml

- 0.25E

-Nx 500 U4.5 S.

number number (E)

(acceptable rane - 0.01 - 022) i-

-5

\\s44 I N90 M 0

l2 IZA O.C)25 0.2-Laboratory con rol standard:

Reference True value Sample Alkalinity

)

% RS = MV I TV 100 standard number (TV) volume Begin l End Total Multiplier (mg CaC03/L)

(acceptable range (mg CaCOAL)

(ml) ml 21 m

ml

-90to 110%.

1 MS t 2(

100 Loo

7;0t-l 8

8l Duplicate s e precision:

Sample Alkalinity

%RPD -

Sample Sample 11 volume Begin End Total Multiplier (mg CaCOQAL)

((S - D) tI(S+DYZII 100 number (ml) ml ml ml

( cceptableranp 410%)

Duplicate I

7 33 8 D e.

Matrix spike recove:

Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplierl (me CaCO,/L) l (mgCaCOlL)

(m ml ml m

I Sample alkalinity (B)

Measured spike value (MV)

% R MV SV s 100 (mCaCOL)

MV=A-B (acceptable range LmC CaCO3,L)

-75 to 125%)

Sample meas-re-ts.,

Sample volume Begin End Total Alkalinity Sample number Sample (m) ml lml ml Multiplier

( [ms CaCOL) l&,.1}03 L

4 AM l°° TL4 2A.

I

_I I

tot I

loU -a-C lkkbS rio 3

t1 a, ah 16.l Z.

t I{o'2-k~3A D

&S

72.

a-A CA.&

1

1.

too i

It io ls-9_

icl lo w

.o Lz.5 5 L.16t

PAC, tot=

,dZo It.!6.

l

\\

5

_~~~~~~~

k, 0

0~

l

°

23. P 15*1.

1 68

~~~~PeU oo jo

~

6b1,7 ri62 I'ir

-k~.iŽ Reviewed by:

ID re

[

Date reviewed:

7{g2

-35 1

Environmental Testing Solutions, LLC Page 1, of Alkalinity (EPA Method 310.1)

Mtrix Water, AWL - 1.0 mg CaCO3/L Analyst r Date anabzed [I4Iov3 Titrate samples to pH = 4.50 SU.

rant anma dmudfil.

terminadon:

PIL~rmallty Normality (N) of KzS0 4 pH Factor or Multiplier Deionized Tltrant che Total-i(5 ml Na2COI x 0.05)/E C

(NY SOOY 100 ml sample water reference standard ml n-mlI 2

5/

E NS 500

- 4.5 S.1.

number nunsber (E)

(aceptable

)

Laboratory control standard:

Reference True value Sample Alkalinity (MV)

%RS-MV/TVx 0

standard number (V) volume Begin End Total Multiplier (mg CaCOVI)

(acceptable range (mg CaCOAL)

(mIl) ml ml nil

- 0to 110%)

81\\\\\\56\\%Co 100 100 too lo.

?-

° 94 995 Duplicate sample precision:

Sample Allinity

/}PD Sample Sample ID volume Begin End Total Multiplier (mg CaCOi/L)

{(S - D) /l(S+D)f211 x 100 number (ml) ml ml ml (acceptable range-10%)

o~~t~~olo2.

tjJ tO.n 2..

XK L

\\8 Dup hi c at.e1*;

l/

D l

_ ~~

~~ ~

.j

2.

Matrix spike recoverv:

Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End j Total Multiplier (mg CaCO/L)

(me CaCOiIL)

(ml) ml l ml l ml l

^e&

~~5b

- 100 21.4 15VO1 Iks o.T Ze Sample akalinity (B)

Measured spike value (MV)

% R MV ISV% 100 (mg CaClL) (

MV - A - B (acceptable range I

(zg

.)

{m CaCO-L) 75 to 125%)

Sample measurements:

Sample volumel Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mu CaCO3/L)

QL;Z 1

T 5

l 59Vo l6. il ot

l.

6-lo,,,ii.c 1VPR 5b;ilo I} IbO 16.*

1Z.A IS4:

tsl4.o1 Z

1 L 1 aoz so. I 3 0t(.-dL I to 3O l 1.O3 5.(o l l

5l+.

I 1

-1

-I I

~.

I I

Reiewed by:

D re i w d Datereviewed:

a-Z4-03

Page j

of

?_

Environmental Testing Solutions, LLC I.

I

.1 I

I Total Hardness (EPA Method 130.2)

Matrix: Water, IDL = 1.0 mg CaCO3IL Date analyed [v2IE L]

rrate ita and mu 4.

determina tion:

Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml gal 0.2./E

( I 50000y 50 Cl sample number number (E)

(acceptable range - 0.013 -

022) 1000 14i0*D t)455 g

°gO.%

lo a

°O

. b102?

?-

Laboratory control standard_

Reference True value Sample Hardness (MV)

% RSS-MV I TV I 100 standard number (TV) volume Begin End Total Multiplier (mg CaCoi/L)

(acceptable range (ny CJCO.)

(mI) ml ml ml

- g0 to 110%)

cl5 k 4 0

50.

1c,- o ID*

0 C

DupIate precision:

Sample

.%Hardness

%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOAL)

((S - D) 1((S4DY21l x 100 number (ml) ml ml ml (acceptable range -

10%)

TZAo M NS5j th 0 S1-7 IZ O_

?,1O -fi lbg 2

_g mg Mg S

j i

.u~o.

I 7C~~

p l

0.

4 picate 4

D2

.3 Matrix spike recov Reference Spike value Sample 1

Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOs/L)

(mL CaCOL)

(ml) ml ml ml Sample hardness (B)

Measured spike value (MV) l

% R = MV / SV I 100

(-zC CoAL)

I MV-A-B I

(acceptable rne I

(me CaCOVL)

= 75 to 125%)

Sample measurements:

Sample volume Begin End Total-Hardness Sample number Sample ID (ml) ml ml nml Multiplier (mg C2COi/L)

Blank l

_ l(should be -0 mg CaCOIL) l 0.0 j

O-T 2-0-2L

.1 l~O 1qO6(tlI{S I-zO 5D 2NL {-S-1

__l Ole 1-O Cl No, V.-

lit --

os l q-L I l

q§s

.1 o (

i -

5

.i l

P IZ.

1610Z~~~~~~bh151

~

5

.;4 Ift S 1

t'.

I a&

_D

_A A

5 L{

{

c 'k.'

1-coctl 5k U

5`

t 3.l 8

_ ~ ~ ~ ~ ~ ~ ~ -

A C-5 ~ :

'elou i u em e ee by D t rt 12Aw 3d 1_ hSQ+S { s ltt~~~~~~~~2..o 12.l.

A 3 b a

.1 Note: If >5ml f titmnt is used. sample must be iluted.

Revieweed by: I C

7 Dat revievved-t^Eo73

I-Environmental Testiogy Solutions. LLC Pagc 2,

of -

t

Environmental Testiof Solutions, LLC Page Z. of

'2..-

Total Hardness (EPA Method 130.2)

Matrix: Water, MDL - 1.0 mg CaCO3/L Analyst CA Z

Date analyzed 6n* 3 j l liar dermination:

Titrnt Normality check *_

Total Normality (N) of EDTA pH Factoror Multiplier reference standard ml mS W02JE 5000 50 1ml sample number cumber (E)Neptc an

- N x 1000 Laboratory control standard.

Reference True value Sample Hardness (MV)

% S - MV I TV x 10l standard number (T) volume Begin End Total Multiplier (mg CaCO2IL)

(acceptable range (C CaCOL)

(MD WI ml ml o

I o%.

1\\3S 10 Q

40 0

22A Uq Z 4.0 2,0-7

%a's Oz.

Duplicate s precision:

san w

w Sample Hardness

%*RPD Sample Sample D volume Begin End Total Multiplier (mg CaCO)IL)

(S - D) II(S4DY21 stoo number (ml) ml ml ml (accepable rane -

10%)

Tb r___

L 2..

1 S

4f Durcat et91

2. o D 4bV l

Matrix spike recovery:

tv 2

Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mgCACOA)

(mg CaCO/L)

(ml) ml ml ml ulple 19s5 lot Zip Sh 13t-l321-Z

-B 0S Sample hardness (B)

Measured spike value (MV)

% R MV/ SV I 100 (mg C2CO3iL)

I MV A - B (acceptable range l

I I

(mt CaCO/L) to 125%)

Sample measurements:

Sample volume Begin End Total.

Hardness Sample number Sample ID (ml) ml

. ml ml Multiplier (me CaCOj/L)

Blank l

___l____

oul

-0 m

CaCOIL l___l____l____

Oo f-j.s Te41r-7-

! s Zq. k 60.5l.

c, I\\

31.

0eo0(7. of 640 1t O

I

\\

3.9 3A 02oC, M. 01 Z

15b94 4 t33 4L Z

{>

fno 3m#

av 11

-b

-A

___r1 Xt

{

-l gS J-r1VA t6liO Im Sb

~

5.

t AJ aoai q -

z Z S3b

~~~1 di.

0L_1q-+

I

~

.t Note: If >I ml of titrnt is used, sample must be diluted.

Reviewed by:

I Dt Datc reviewedi l L4 Z60

Environmental Testing Solutions, LLC

-Sl S

Page -Jqa Page.... of Total Residual Chlorine (EPA Method 330.5)

Matrix: Water, MDL - 0.10 mg/L Meter: Accumet Model AR25 pHlonI Meter Analyst CWt Date analyzed Olo * + 03 Iodide reagent:

g CB t Acid reagent: 4&I Le 6 I3 Calibration:

Sq 0.10 mgfL I

1.00 me/L I

I Reference standard number I %V55 I Sq I

l Notc: For samples with a residual Chlorine of 1l.O mglL. the calibration range must be adjusted to brvdet the chlorine levels ofthe samples.

Laboratory control standard:

Reference standard True value (TV)

Measured value (MV)

% RS - MlV/ TV x 100 number (milL)

(mgIL)

(acceptable rnge -90 to 110%)

1 3 A-0.50

.1 S* 9 tqB Dt plicate sample precision:

Sample Sample ID Sample characteristics Residual chlorine

%RPD = ((S - D) II(S+D)/1]}

100 number (ma9L)

(acceptable range -

10%)

_Duplicate D (p o Samplc measurements:

Sample Sample ED Sample characteristics Residual chlorine number (m/L)

MEM Blank (should be = <0.10 mg1L)

<.0.O j5 bv1 ot ;74r-S 1J IQ I 4V c6t

&io~.

t riS

.IW t.

KfMY.

t\\(s:aL 4t b I cAr

{~a.

9 cs (0t. O ob t zIO~ao 1- - 0^

L aiD O-O S9 kObt 6 - 2 0.

-P c6t-v I

JA; J..

(,a.0o 4:

J.,o.

.a CI.62-<

Note: All samples were analyzed in excess or EPA rcormnendcd holding timc (I 5 minutes) unlcs otherwise noted.

Laboratory control standard:

Reference standard True value (TV)

Mcsasured value (Vm

% RS -hV /TV 100 number (mgIL)

(m lL)

(acceptable range - 90 to 110%)

M5$ib 134 0.50 O.'t 8

I Reviewed by L Date reviewed _

A I

6-Zi(

i n

Environmental Testing Solutions. LLC

- 4q 2 1" -

Page Ci 12 Page I of I

r' W.-

Total Residual Chlorine (EPA Method 330.5)

Matrix: Water, MDL - 0.10 mglL Meter: Accumet Model AR25 pWon Meter

. Ww;-::

. t' t:,

S s.^1 Analyst

(

k Date analyzed I j6 j.

3 Calibration:

Iodide reagent:

08q 1

Acid reagent: I f OR _ a W

g7-W 0.10 mg L I

1.00 m/L IReference. standard number IbcsI55t4 I I-55134 I

note: For samples with a residual chlorine of > 1.0 mgfL. the calibration range must bc adjusted to bracket the chlonne levels of the samples.

Laboratory control standard:

F

,s, r

P.,ct aM.'

^:

AT,.

r;'

'.^:,,-.

S:

So, S.:b t:

ibi-m w:

.k

o s

Z Reference standhrd True value (TV)

Measured value (MV)

. RS htV / TVx 100 number I

(mi/L)

(m/L)

(acceptable range - 90 to 110%)

o55 j3.s 0.50 O.A 7.2 Duplicate sampIe precision:

Sample Sample ID Sample characteristics Reidual chlorine

%RPD (S - D) 1(S+D)12J) x 100 number (mj/L)

(acceptable range 1

10%)

t3o0I1&.Lg

-P.

¢4 S-174 uplicate

-f D

Samle measurements:

Sample Samplc ID Sample characteristics Residual chlorine number (mRlL) 3;_t Blank (should bc = < 0.10 mgL)

._C O

s 030o'5.l 9 -O

\\)/

4Lt adrk {C-&,ho

-aA0L p-Ob

]

Note: All samples vre analyzcd in excess of EPA recommended holding time (15 minutes) unless othernise noted.

Laboratory control stamndard:

Refercnce standard True value (TV)

Measured value (MV)

% RS =MV TV s 100 number (mgIL)

(mn)

(acceptable range - 90 to 110/6) 5D V54 0.50 0).,A(O?>

43.

741 Reviewed by Date reviewed

0.

1 A(.~- I

Environmental Testine Solutions. LLC

- -4.

Page 33I -A Page -..L.... of j r_

Tk

4-...

if,.

C.,

E..

Total Residual Chlorine (EPA Method 330.5)

Matrix: Water, MDL - 0.10 mg/L Meter. Accumet Model AR25 pM/Ion Meter Analyst Dstc analyzed Oto. Zl 03 Iodide reagent L

L Acid reagent 9 oiN0I5 Calibration:

I I

O.tO mg/L I

1.00 mg/L Reference standard number I

1 a31 I

SSl I

Note: For samples with a residual chlorine of > 1.0 mg/L the calibration range must be adjusted to bracket the chlorine levels ofthe samples.

r r

S.

e r

.so..

Laboratory control standard:

Reference standard True value (TV)

Measured value (MV) l

%RS-MVITVxIOO number (miL)

(m:/L)

(acceptable range - 90 tot O%)

I N 6604 0.50 O. 60Z 1O1 LII Duplicate sample precision:

Sample Sample ED Sample characteristics Residual chlorine

%RPD = ((S - Di I((S+D)/2j) I 100 number (mg/L)

(acceptable range -

t to%.)

O2bZl. it raj on WuAF s

_C____o__e_

4, Duplicate D.O.O50 S Sanple measurements:

Sample Sample ID Sample characteristics Residual chlorine number (mgtIL)

Blank (shouldbc=C0.l0mg/L) i

, 00CIs4$

mol.

sA-m*h d

d 413-0azo 0MCC7.. OS

5.

n 11ku Nt 000 Z0.0OZ 2(a ob2-z d

do O-2,M.7.

02..tk#

v 0

4.

t).

OQe4 L tJ QM6I2.03 Ot7AQj-',- - w Nh no C.0c40d, CkaWiZ' L0.00WZO A

IWO z, CA RP!(ess -

LCe n6 (o

1 (o

.O.OO4s

.O21 p P

ri P

P1t, Note: All samnples werc analyzed in excess of EPA rccommendcd holding dine (15 minutes) unless otherwise noted.

Laboratory control standard:

Reference standard True value (TV) r Mcasured value (bV)

% RS - bV I TV x 100 number (rng/L)

(mngIL)

(acceptable range - 90 to 110%)

hNSt ;

0.50

0. 4C*?

.6

'I Reviewed by A

Date reviewed I

Sequoyah Nuclear Plant Biomonitoring June 17 - 24, 2003 Appendix D Reference Toxicant Test and Control Chart

Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 1.0 0,9 0.8

. 0.7 0.6 T-- I 7 I I

I I

I State and USEPA Mandated Control Limits Limits Set According to; 2 Standard Deviations

~~~ '

, ~~~~~.....................................................

Q Insq Q

0.5 0.4 1.0 0.9 0.8 I 0.7 0.6 0.5 I

I I

USEPA Warning and Control Limits Limits Set According to 10h and 25'k Percentile CVs

~

0.4

~~s 4 4

~~~~

a-a 6

a a',

Test date

-i-7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

Central Tendency (mean IC25)

Warning Limits (mean ICs SA.10)

Control Limits (mean IC2, t SA25 or 2 Standard Deviations)

Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephates promelas using Moderately Hard Synthetic Water Test number Test date 7.dy ICu CT (t 3CCI/L)

(giL Ka)

I 09-10-02 0.61 2

09-17-02 0.64 0.63 3

10401-02 0.60 0.62 4

10-01-02 0.47 0.53 5

10-03-02 0.53 0.57 6

10-15-02 0.66 0.59 7

10-22-02 0.64 0.59 t

11-05-02 0.59 0.59 9

12-03-02 0.61 0.59 10 12-03-02 0.52 0.59 I I 01-07-03 0.64 0.59 12 01-14-03 0.61 0.59 13 02-04-03 0.64 0.60 14 03-1t-03 0.65 0.60 IS 03-11-03 0.64

0.60 16 04-04-03 0.50 0.60 17 04-15-03 0.56 0.59 1

04-29-03 0.59 0.59 19 0S-29-03 0.64 0.60 20 06-17-03 0.60 0.60 State and USEPA Laboratory Laboratory S

Control Limits S. 11 Warning Limits SA3 Control Limits SAM CT-2S CT-2S CT S.1 CT S.

CT -SA, CT + SA.3a 0.02 0.59 0.67 0.03 0.55 0.70 0.13 0.50 0.76 0.24 0.02 0.53 0.66 0.07 0.55 0.70 0.13 0.49 0.75 0.24 0.03 0.43 0.74 0.07 0.51 0.65 0.12 0.46 0.71 0.22 0.07 0.43 0.71 0.07 0.50 0.64 0.12 0.45 0.69 0.22 0.07 0.44 0.73 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.07 0.45 0.73 0.07 0.52 0.66 0.12 0.47 0.72 0.23 0.06 0.46 0.72 0.07 0.52.

0.66 0.12 0.47 0.72 0.23 0.06 0.47 0.71 0.07 0.52 0.67 0.12 0.47 0.72 0.23 0.06 0.46 0.71 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.06 0.47 0.71 0.07 0.52 0.66 0.12 0.47 0.72 0.22 0.06 0.48 0.71 0.07 0.52 0.66 0.12 0.47 72 0.23 0.06 0.43 0.71 0.07 0.52 0.67 0.13 0.47 0.72 0.23 0.06 0.49 0.71 0.07 0.53 0.67 0.13 0.47 0.73 0.23 0.06 0.49.

0.71 0.07 0.53 0.63 0.13 0.41 0.73 0.23 0.06 0.41 0.72 0.07 0.52 0.67 0.13 0.47 0.72 0.23 0.06 0.43 0.71 0.07 0.52 0.67 0.12 0.47 0.72 0.23 0.06 0.41 0.71 0.07 0.52 0.67 0.12 0.47 0.72 0.23 0.06 0.43 0.71 0.07 0.53 0.67 0.13 0.47 0.72 0.23 0.05 0.49 0.71 0.07 0.53 0.67 0.13 0.47 0.72 0.23 USEPA Warning Limits S.t CT S.'

CT + S^

USEPA Control iUmits CT-. S CT 8,0 Cv 0.39 0.33 0.36 0.35 0.36 0.37 0.37 0.37 0.36 0.37 0.37 0.37 0.37 0.37 0.37 0.37 0.37 0.37 0.37 0.37 0.23 0.36 0.21 0.t0 0.26 0.79 0.26 0.3 1

0.26 0.32 027 0.32 0.27 0.12 0.27 0.33 0.26 0.32 0.27 0.2.

0.27 0.32 0.27 0.33 0.27 0.33 0.27 0n 0.27 0.32 0.27 0.32 0.27 0.32 0.27 0.32 0.27 0.35 0.34 0.32 0.33 032 0.33 0.33 0.33 Q32 033 Q33 0.33 0.33 Q33 0.33 0.33 0.33 0.33 0.33 0.91 0.03 0.90 0.03 0.35 0.13 0.33 0.3 3

0.32 0.36 0.12 0.36 0.13 0.36 0.10 1Us 0.31 0.36 0.10 0.36 0.10 0.37

. 0.30 0.7 0 09 0.37 0.09 0.36 0.30 0.26 0.10 0196 0.10 0.37 0.09 0.37 0.09 E:

7-d IC

7-day 25% inhbition concenntraio An esimation t the concentratio of potassium chloride that wold cause a 25%rduct in Pisnephas owth for Ithe lst popuat CT I Central tendency (mean ICa SO Standard deviation of the IC,, values.

Laboratory Control and Warning Umits Laboratrycontrol andw ningmits wer sabished usingthestandard devtion othe ICs values correspondingto die lOth and 25th percentilc CVt.

Thes rangesarcmore astinent thanthceomtrlnd wantigmits recommended by USEPA for Ihe test method and endpoinL S

- Standard deviation corresponding to the 10 prceniile CV. (SA.

0.12)

SAn - Standard deviation eormponding toth e25' percentile CV. (St O 0.231)

USEPA Control and Warnin LiUmits S-.n Standard deviation corresponding lo the 75' perentile CV. ( S

-0.33)

S.N O Standard deviation cormsponding to the 90' pereentile CV. (S. -0.45)

CV - CoefTtcinl of ariation of the IC., vales USEPA. 20o0.

WutandkgnAd Aomu arot M

Vambityin Whoe EfucalTa*

Appstiont ldas.

int POut Dise Eu ni ags... Ent.FPA433-R0.0 US Envionunea PmtsdeaAaeCy.

miC*.OtL a'gansjuu obandfiva Aquaetic slialyesins. fle.

0614iOlsl

.4.~~~~~~~

g -

e~~~~~~~

Environimental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 1.0 0.9 0.8 0%

,..W U4 t

tn.

N U

.0--4 Po r-

0.7 0.6 0.5 0.4

-~

~

~~-

I l

I I

......1 0.3 0.2 7-day IC25= 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pirnephales growth for the test population.

Central Tendency (mean IC2 )

..- Laboratory Warning Limits (mean IC25 + SA10, SA

= 0.12)

Laboratory Control Limits (mean IC2 SA2S, SA 2S= 0.21)

USEPA Warning Limits (mean iC25 i SA?7, SA75 = 0.38)

USEPA Control Limits (mean 1C25+ SA90 SA 90 = 0.45) 25' A.901 A.90~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~'

Av.-

Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephales promelas using Moderately Hard Synthetic Water Test number Test date c"

X Control Mean Growth cr Cv cr MSD PMSD cr

(%)

(mgflarvae) for Control Growth (Mg/larma) for Control Co/)

Growth CV (-A) for PMSD (%)*

I.

09-10-02 2

09-17-02 3

10-01-02 4

10-01-02 5

10-08-02 6

10-15-02 7

10-22-02 8

11-05-02 9

12-03-02 10 12-03-02 I I 01-07-03 12 01-14-03 13 02-04-03 14 03-18-03 15 03-18-03 16 04-08-03 17 04-15-03 18 04-29-03 19 05-29-03 20 06-17-03 Arote:

CV 100 100 91.5 100 97.5 100 100 100 100 100 100 100 97.5 100 100 100 100 97.5 100 97.5 0.854 0.824 0.839 0.750 0.809 0.975 0.851 0.929 0.866 1.037 0.895 0.822 0.884 0.874 0.883 0.852 0.880 0.668 0.858 0.886 0.861 0.677 0.846 0.933 0.852 0.838 0.85 I 0.803 0.848 1.083 0.863 0.892 0.864 1.02 1 0.873 1.005 0.880 0.888 0.880 1.2 13.4 18.4 12.7 8.0 16.9 10.6 2.8 9.1 10.4 4.1 3.0 14.1 8.0 21.3 6.1 17.0 6.7 7.9 4.2 7.3 11.0 11.4 10.7 11.8 11.6 10.5 10.3 10.4 9.8 9.2 9.6 9.5 10.3 10.0 10.4 10.2 10.1 9.8 0.11 0.12 0.19 0.13 0.18 0.23 0.13 0.12 0.12 0.15 0.14 0.07 0.15 0.15 0.21 0.09 0.17 0.18 0.11 0.18 12.5 14.6 25.4 12.8 19.5 21.9 15.6 13.8 13.7 22.4 15.7 11.0 16.5 18.5 26.5 8.0 18.7 17.5 11.1 20.7 13.6 17.5 16.3 17.0 17.8 17.5 17.0 16.6 17.2 17.1 16.6 16.6 16.7 17.4 16.8 16.9 16.9 16.6 16.8

- Coefficient of variation for control growth.

On average, the CV for control growth is 9.S% in nvinmental Testing Solutions LLC Pimephales chronic toxicity tests.

Lower CV bound determined by USEPA (10 percentile) - 3.5Y.

Upper CV bound determined by USEPA (90"' percentile) - 20%

MSD - Minimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision. TMe PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole eftluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions. LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 16.8% from the control.

Lower PN1SD bound determined by USEPA t10 percentile) - 9.4%.

Upper PMSD bound determined by USEPA (90" percentile) - 35%.

CT - Central Tendancy (mean Control Growth. CV. or PNMSD)

The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.

USEPA. 2000. Understanding and Accounting for Mvethod Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA433-R-00-003. US Environmental Protection Agency. Cincinnati. OH.

Organisns obtainedfirom Aquatic BioSystems. Inc.

06-17-03ids

E Tsi n-S Environmental Testing Solutions,. LLC

- I f Pimephalespromelas Control Growth and Coefficient of Variation in Potassium Chloride Chronic Reference Toxicant Tests 1.50 1.25 o

0 o._

1.00 0.75 0.50

~~~~~~~~~.............................................................

Minimum Acceptance Criteria (0.25 mg per surviving larvae)

I I

I 0.25 C

0

,=

I-q-

o C

U U

U C

C-)

40 30 20 10 0

North Carolina Acceptance Limit (< 40.0°/a)

Kentucky Acceptance Limit (< 30.0%)71 i ~...........................................

' "' - ~~~~. -'.

.,,1 8,,

~.......

40.,

-O W4 Test date Control Growth or Coefficient of Variation (CV)

Central Tendency (mean Control Growth or CV)

Control Limits (mean Control Growth or CV + 2 Standard Deviations)

Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant-Control Chart for Pimeplalespromelas using Moderately Hard Synthetic Water 40 30 1.11%

0 N<

01 1 PCn 04 J'I 20 10 0

t-o

-eet 49sh

/tvl7 11 E B,

Pe Test date

+o-- PIMSD percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

Central Tcndency (mean PMSD)

Lower and Upper PMSD Boundj Lower PMSD 3ound (10h percentile) = 9.4%, Upper PMSD Bound (90" percentile) - 35%

(Lower and upper PMSD bounds were determined by USEIA for the method and endpoint.)

I

..... I

Lrnvironmnental I esting Solutions, LLC aelr Page of 5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas PpKCICR Test Number Dilation preparation information:

Comments:

KCI CHM number:

CAK\\

C6-1_

Stock preparation:

50 g KCUL: Dissolve 50g KCI in 1.L Deionized water Dilution prep (mgL) 300 450 600 750 900 Stock volume (mt.)

6 9

12 Is I

Diluent volume (mL) 994 991 958 985 982 Total volume (mL) 1000 1000 1000 1000 1000 Test organism. information:

Test information:

Organism age:

2.

- ZS.?3 o.J a3 Randomizing template:

t Date and times organisms CEc-l-Oc I11 rQ 10 Incubator number:

were born between:

M V 3

Organism source:

AE:SePrcA D-vi-c3 Arfemia lot number:

6a043 s Transfer bowl information:

pH =

Tcmperature =

TC Total drying time:

q W cc)

ZIA.1 Date / Time in: toWL403V.

-3 Average transfer volume:

lb q Q Date J Time out: 010253 I O

___O ven tem perature:

Dailyfeeding and renewal information:

Day Date Morning Afternoon Test initiation, Control water Analyst feeding feeding renewal, or batch used time time termination time A>

0 k -cc6 r.-wv-O_

I'ii 1

C-t-o05 Oq Ob

%Soo 0

- 1 2

o~b 1t-A3 O 30 vsOo 13o o(5-n-3o 3

06-03 1oia 1Ib35 1347o

~

1 4

~

'i ioj

____v 4

0 621 Oi~

D (En, 1

ip 3_ 7 II i_

5 Z06-24-03 Qq 13 I'S 14

_-zl o

7 6

'2A -0 134 O&Z Kor I Control information:

Acccptancc critcria I Summary of test endpoints:

I/

Mortality:

1.51 l

20%

7-day LCs 0

I i

Average weight pcr initial larvae:

I 0.00%3 N

IOEC I

3W Avcragc weight per surviving larvae:

O.

I _

0.25 im&l;arve LOEC

_ 4..

IChV 3 tVq.q

. RC25 r

2. !

-c v

vJ~mULIU~aJ9 h~LA1...

elotSz--

Paze 2 of 5 r.v r

^

e.

.-yv t

PpKCICR Test Number

!L

.t*.

Survival and Growth Data Day CONTROL 300 mg KCU'L 0 mg KCIIL

______A B

D E

F G

I J

K L

0.

° Jo 10 10

/0 10 10 10 1

/0 10 10 10 10

/0 (0

10 t(

10

/0 10 2

t 1 1I C

)

0/0

/0 c"' /0 1o

/0 3

/0Oq

/0

/() /61 (J/

G 4__o_

_o i

//

/O /0

/0

/o0 /

5 to '._

o o9 l

to 1 0 c

0to (bt0/0 /09 6

/o 9

10

/1 O0 /o /0

/0 '

lol 7

.___ 10 9

/0(010 o

/69q10 9e A Pan weiglat

-mg 1

IX-5000

- Pan Larvae wig~lt (mg) 4B f

i ^.

S Et A

7 Larvac weight (mg) - A-B l

AI L 1 I <

OA V

I A I A I,

IIt_

C.

6 OE

\\ 3A N

I

.~

C9 oF'e' o

0'3.0 W-l" ID

'IC

-&sac1at viewcd LA t-F wqv qLoW

-C,,b I.

g*14W,cE.j3 OxW jpo-*%

451-O.U Comments:

jnvirolmental Testing Solutions, LLC Page 3 of 5 PpKCICR Test Number:

L Survival and Growth Data Day 600 me KCIIL 750 mg KCtlL 900 mg KCUL 1M1 NJ lO Q

R S

T U

V W

X r, ~~

(z)

,t

/

j

/C

(

/0 (0

/0 fO 10 /0

/d

,_ _ _ _ _ _ _ _ (0 10 r0

/

,O to

&(

to 2

.C

-Lg 3

Z-$A t 4 84 &

l 4

6

&4 104 S

7 t

1~~~~~~~o

/O Lit6 6

9 7

A-Pan cight(ng)

B - Pan Larvae weight (mg)

H Larvae wcight (mg)

A B S\\%A 0.,

'IA.

1e 0 o-i4If1 OO(s3 mvS i

K.

O' Calculations and ta reviewed:

~~~~~~~~~~S-

r.

_Z ae WAA

.J4 rzoC rALee t\\)9%AX

.v

.i.. i:

L-

Comments:

Environmental Testing Solutions, LLC Chronic Wholc Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)

Species: Pimephatespromelas Quality Control

Verifieation of Data Entry, Calculations, and Statistical Analyses Test number.

PpKCICR H St Test date:

June 17-24,2003 Revelwed I

C"esuuad (mg/l.

Rtapksc 1.1. sambor of FlaaI numberot A

an wdght U - P LhrVea La9" wigi (MC Wdtight/iN1,5 a.a 0,16.48n^

bmes wiht(g) of Perest gadacdo. from kCI) lara larvae (mg) wdfight (mg)

-A-of l.rlat (MR)

Ml)

Wattaga 0) ctrnl 4%)

A 10 10 15.24 23.980 1.696 0.t696 Control 10 9

15.160 24.560 9.400 0.9400 97.5 0.8873 4.2 Not applicable C

10 10 14.309 23.690 3.331 0331 D

10 10 14.647 23.10 8.533 0533 B

10 10 15.106 22.630 7.524 0.7524

~~oo P~~~

I0 1 0 14.874 22.360 7.486 0.7486970742.26.

300 C

10 10 14.66 220 3.054 0.1054 97.5

.745 7

16.

if 10 9

15.047 21.790 6.743 0.6743 I

I10 10 14.791 22.90 3.099 0.3099 45eOI iJ 10 9

14.659 20.930 6.271 0.6271 93e 0.709 11 1.0 450 1~~~

I0 10 I15.303 22.760 7.457 0.7457900001.22.

L 10 9

14 01 21.010 6.209 0.6209 M

I.0 15.151 21.030 5.179 0.5879 600 N

10 ID 14 35 223107.975 0.7975 31.5 0.6710 21.4 24.4 o

to to 14.766 23.410 8.644 0.3644 P

Io 0 14.798 19.140 4.342 0.4342 Q

_ 10 4

15.110 17.700 2.590 0.2590 7se R

10 6

15.094 1t.370 3.272 0 3272 50.0 0.3223 36.4 63.7 ICl 6

15.042 Ill it0 4143 0.4343 T

10 4

14.910 17.090 2.10 0.210 U

10 2

15.053 16.520 1.467 0.1467 900

-V 0

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0 0

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Percent Minimun Significant Difference PMSD is a asureoftest precision.

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The lower and upper bounds were calculated by the USEPA using 205 teats conducted fron 19 i eboratoriin for Pineplee powth in chronic reference toxicant tests.

USEPA 2000. WJhdestanding and Accounting for Method Variability in Whole E fent Toxicity Applications Under the Nuational Pollutant Dischige Elimination Program PA-33-RO003. US EvmimamIwal Protection Agency. Cincinnati, Oi Organirss obtainedfram Aquatic BiaSystent, /inc 06-17M.3.

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Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.

Central Tendency (mean IC25)

Warning Limits (mean IC25 + Ss 0 )

.Control Limits (mean IC25 S.

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i Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Cerioalaphnia itubia using Moderately Hard Synthetic Water I

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CT-2S CT+2S Laboratory Warning Limits CT - S,,

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7-d ICas

7day 25% inhibition concentration An estimation of the concentration or sodium chloride that would cause s 25% reduction in Ceriodaphnia reproduction for the let population, CT - Central tendency (mean ICI,4 S - Standard deviation of the ICu values.

Laboratory Control and Warning Limits Laboratory control and waming limits were estblished using the fstndard deviation of the IC25values correspooding to the 10th and 25th percentile CVs.

These ranla are tr stringent thn th ecotol ian waming limita recommended by USEPA for the test method and endpoint.

SAa - Standard deviation corresponding to the 10 percentile CV. (SA,1. -0.03)

S

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Standard deviation coreuponding to the 25 ' percentile CV. (S s -0. 17)

USEPA, Control and Yarning Limits SA75

Standard deviation correaponding !o the 75* percenile CV. (S.3 0.45)

S- - Standard deviation corresponding to the 90' percentile CV. (S r, 0.62)

CV - Coefficient of variation of the IC%$ values USEPA. 2000. Underntanding and Accounting for Method Variability in Whole Efiluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-133-R00-003. US EnvrrOnmetl Protection Agency. Cinclinati. OIL

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Central Tendency (mean C25)

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c SD PMSD CT number Strvival ReproductionM or Control Mean for Control (16)

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1 04-09-02 100 26.2 8.6 2.8 10.7 2

05-07-02 100 27.3 26.8 12.3 10.5 2.3 8.4 9.5 3

064-02 100 26.0 26.5 8.1 9.7 3.8 14.7 11.2 4

07-09-02 100 29.5 27.3 9.9 9.7 3.5 11.7 11.4 5

08-06-02 100 28.4 27.5 8.0 9.4 2.7 9.5 1 1.0 6

09-04-02 100 31.4 28.1 10.4 9.6 3.0 9.5 10.7 7

10-08-2 100 31.1 28.6 6.7 9.2 2.9 9.4 10.5 8

11-05-02 100 29.5 28.7 9.2 9.2 2.5 8.4 10.3 9

1203-02 90 34.0 29.3 8.0 9.0 2.7 8.0 10.0 10 12-03-02 100 33.2 29.7 6.2 8.7 3.3 9.9 10.0 I1 12-04-02 100 32.5 29.9 6.0 8.5 3.2 9.8 10.0 12 12-06-02 100 29.7 29.9 11.0 8.7 3.0.

10.0 10.0 L3 12-11-02 100 33.8 30.2 13.7 9.1 2.9 8.5 9.9 14 12-18-02 100 30.5 30.2 7.4 9.0 2.9 9.4 9.8 15 01-07-03 100 33.2 30.4 7.0 8.8 2.9 8.6 9.8 16 02-04-03 100 32.3 30.5 8.1 8.8 2.7 8.4 9.7 17 03-05-03 100 28.7 30.4 5.1 8.6 3.5 12.1 9.8 18 04-11-03 100 26.3 30.2 6.2 8.4 2.5 9.6 9.8 19 05-06-03 100 27.6 30.1 10.8 8.6 3.2 11.5 9.9 20 06-04-03 100 25.9 29.9 5.9 8.4 2.6 10.1 9.9 Note:

CV - Coefficient ofvariation for control reproduction.

On average, the CV for control reproduction is 8.4% in Environmental Testing Solutions. LLC Canodophnia chronic Lower CV bound determined by USEPA (10 percentile) - 8.9%

Upper CV bound determined by USEPA (90 percentile) -42%

MSD Minimum Significant Difference P&MSD - Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On avenge, a significant differcnce occurs for Environmental Testing Solutions. LLC chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.9%

from the control.

Lower PMSD bound determined by USEPA(10* percentile) - 11I%

Upper PMSD bound determined by USEPA (90' percentile) - 37%.

CT-Central Tendncy (Mean Control Reproduction, CV, or PMSD)

The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 aboratories for Ceriodaphnia reproduction in chronie reFerence toxicant tests.

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program EPA-33-R40403. US Environmental Protection Agency. Cincinnati. OH.

06-04-03ixf

Environmental Testing Solutions, LLC Ceriodaphnia dubia Control Reproduction and Coefficient of Variation in Sodium Chloride Chronic Reference Toxicant Tests 40 35 C__

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- Central Tendency (mean Control Reproduction or CV)

.Control Limits (mean Control Reproduction or CV -2 Standard Deviations)

Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium4 Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water 50 45 40 35 f 30

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Test date PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

Central Tendency (mean PMSD)

Lower and Upper PMSD Bounds Lower PIMSD Bound (I0' percentile) = I%. Upper PMSD Bound (90' percentile) - 37%

(Lower and upper l'MSD bounds were determined by USEPA for the method and endpoint.),

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Environmental Testing Solutions, LLC 1.4 Page of 6 Environmentzl Testing Solutions, LLC Page Iof6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphnia dubia CdNaCLCR :

Dilution preparation information:

Comments:

NaCI CHM number.

(aO Stock preparation:

OO g NaCI/l (dssolve 50 g NaCI in 500 ml dcionized *ater)

Dilution prep (mJ L) 600 3oo 1000 1200 1400 Stock volume (mL) 9 12 15 I

.1 21 Diluent volume (mL) 1491 14S8 14S5 1482 1479 Total volume(mL) 1500 1500 0

1500 1500 1500 Test organism information:

Test information:

Organism age:

4 zq -loteL Randomizing template:

YAie-Date and times organisms S

Incubator number and were born betveen:

M-04. -6 0t1 10Z shelf location:

Organism source:

6S-Z1 CaPS A-b YCT batch:

f

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pH =E.Q3 Temperature =2 q,0 Selenastrum batch: f5 OS-j23C Daily renewal information:

Day Date Test initiation, Control water batch Analyst reniewal, or used termination time Ob-O5-03 \\3c~~t 66 OS-O

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Efnvironmental Testing Solutions, LLC Page 2 of 6 Species: Ceriodaphnia dubia CdNaCLCR #:

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I-Environmental Testing Solutions, LLC Page 3 of 6 Species: Ceriodaplnia dubia CdNaCLCR #: 1f 800 mg NaCl/L Survival and Reproduction Data

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Z3,q

% Reduction from Control:

4.1 -'

Environmental Testing Solutions, LLC Page 4 of 6 Species: Ceriodaphnia dubia CdNaCLCR#:

j 1 1200 mgNaCf/L Suryival and Reproduction Data Replicate numher DaI1 2

3 4

S 6

7 a

9 2

Young produced I

0 Adult mortality Z

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-l _______

Replicate number I

Day Y

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6 T 7

8 I

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Environmental Testing Solutions, LLC Chronic Whole ElMuent Toxicity Test (EPA-821-R-02-013, Method 1002.0)

Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number.

CdNaCICR 40 Test dates:

June 4-11 2003 Revewed by: K

&/

Concentration Replicate number Survival Average reproduction Ceeffkde f I-e-rdti-rr (mgIL NaCI) 2 3

4 5

6 7

8 9

()

(offspring/female)

T OW60 19r1t

(%)

Control 23 26 26 27 25 27 23 27 24 26 100 25.9 59 Not applicable 600 32 27 24 24 25 27 25 25 27 27 1300 26.3 9.0

.1.5 800 25 24 27 21 30 25 27 27 27 23 100 25.6 100 1.2 1000 24 22 26 24 24 22 22 26 24 25 300 23.9 6.4 7.7 1200 6

5 14 14 S

13 12 14 12 15 100 11.3 321 564 1400 5

5 10

.10 S

12 3

12 9

10 300 8.4 36.9 67.6 Dunnet's MSD value:

PMSD:

2.621 10.1 MSD Minimum Significant Difference PMSD

Percent Minimum Significant Difference PMSD is a measure of teat precision. The PMSD is the minimum percent difference between the control and tretmenl thlat can be declared utAditieally significant in a whole emuent toxicity tLest. On average, a significant difference occurs for Environmental Testing Solutiona LLC ehronie txicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.9% from the control.

Lower PMSD bound determined by USEPA (10' percentile) -

1%

tipper PMSD bound detrnined by USEIPA (90s percentile) - 37%

lihc lower and upper bolnds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cerodopahn reproduction in chronic reference toxicant testa USEPA. 2000. Understanding and Accounting for Method Variability in hole Euent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Enviromnentl Proection Agency. Cincinnati. OIL

Environmental Testing Solutions, LLC Verification of Ceriodlaphnia Reproduction Totals Control

~~~~~~R pacute number-Day l 1 2

3 4

5 6

7 8

9 10 0

0 0

0 2

0 0

0 3

0 0

0 4

3 3

4 4

4 3

4 5

4 4

3S 5

9

11 12 10 10 II X

10 t0 102 6

0 00 0

0 0

.0 7

II 12 3I 11 II 34 33 14 10 12 119 Total 23 26 26 27 25 27 28 27 24 26 259 600 mg NaCIL Rceplicate number Day l

-j--

n m 7r-Total 0

0 0

O O IO 0

0 0

0 2

0 0 0 0 0 0

0 0

0 0 0

3 0

0 0

00 0

0 0

0 4

4 5

4 4

4 3

-4 4

4 4

40 5

13 12 10 8

10 10 I I 10 12 10 106 6

0 0

0 7

15 10I !° 12 1 I 14 10 13 117 Total 32 27 24 24 25 27 25 25 27 27 263 800 mg NaC/L Replicate number Day

-j 2j-3 1 4 5

6 7f 8

9~

10 Total r

1' 0

0 O

O o

o o

0 o o

0 2

0 0

.0 0

0 0

0 3

0 0

0 4

5 4

4 3

3 5

4 4

40 5

10 300 7

12 10 11 1I 13 10 04 6

0 0

0 7

10 10 13 33 1

10 1212 09 32 Total 25 24 27 21 30 25 27 271 271 23 256 1000 mg NaCI/L Re licate number Day 1

2 3

4 5

6 1 7 a

9 1

t9 Total 1

0 0

0 2

0 0 0

0 0

0 3

0 0

0 4

1 4 3

3 4

4 2

3 4

4 4

35 1

5 10 i I 10 10 a

10 9

12 9

7 96 6

0-0 0

0 0

0 0 0 0

7 108 3

30 32 30 3 0 30331410 Total 24

_22 26 24 24 22 22 26 24 25 239 1200 mg NaCI/L Replicae number Day

-j--- ~

~ ~

6 7

B-

~

Total

-aS I

I I

2 3 14 1

6 17 9

1 0 I 1 0

0 0

0 01 0 0

0 0

2 0

0 0

0 0 0 0

3 0

0 0

4 2

0 4

4 0

3 3

4 2

4 26 S

0 4

7 0 3 S

0L 10 30 6 6

4 1

0 0

0 0I 0

0 0

13 7

0 0

3 0

5 2

1 0

0 6

Total 6

5 14 14 13 12 14 12 15 113 1400 mg NaC/L Dy lRe licate number Ttl Day T3T4557 61 7 a

9 1i Tta I

0 O

0 0

0 O

0 0

2 0

0.

0 0

0 3

0 0

0 4

1 3

2 3

3 1 2

4 3

3 26 5

40605 1

507 34 6

0 2

2 7 1 0

0 3

0 21 7

0 0

0 3

0 0

0 3

Total S

S 10 10 12 3

10 1.

'4 4

Reprodudtion.2 I

EA ~ ~

~

I ax

~

~ ~ ~ ~ ~

Il*1;g l

Y 888l 600 t-Y l

boo

^°8"

_n A880 00880

co n

0!!

0 x V v n

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'I 400 I*w~

~

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Environmental Testing Solutions, LLC Statistical Analyses Used for PMSD calculation only.

Cerlodaphniz Survival and Reproduction Test-Reproduction Sta Date:

614/03 Test D:

CdNaCICR Sample ID:

REF-Refoxicant End Daze:

l 1103 Lab ID:

ETS-Env. Testing Solutions Sample Type:

NACL-Sodiwn clLoride Sample Date Protocol:

CHRON1C-(EPA-82l.R42013)

Test Species:

CD-Ceriodaphnia dubia Conc.%

I 2

3 4

S

.6 7

S 9

t0 D-Contl 23.000 26.000 26.000 27.000 25.000 27.000

, 21.000 27.000 24.000 26.000 600 32.000 27.0 24.000 24.000 25.000 27.000 25.000 25.000 27.000 27.000 800 25.000 24.000 27.000 21.000 30.000 25.000 27.000 27.000 27.000 23.000 1000 24.000 22.000 26.000 24.000 24.000 22.000 22.000 26.0W 24.000 25.000 1200 6000

.000 14.000 14.000

.000 13.000 IL000 14.000 12.000 IS.000 1400 5.000 5.000 10.000 10.000

.000 12.000 3.000 12.000 9.000 10.000 Transform: Untransormned I-Tailed Conc.%

Mean N-Mean.

tean MIn MaE CV%

N t-Stat Critica M SD D0Control 25.900 1.0000 25.900 23.000 2t.000 5.384 10 600 26.300 1.0154 26.300 24.000 32.000 S.971 10

.0.349 227 2.621 100 25.600 0.934 25.600 21.000 30.000 9.951 10 0.262 227 2.621 000 23.900 0.9223 23.900 22.000 26.000 6.376 10 1.745 2.287 2.621

1200 11.300 0.4363 11.300 5.000 15.000 31057 10 12.736 2.287 2.621

1400 8.400 0.3243 8.400 3.000 12.000 36.36 10 15.266 2.237 2.621 Auxiliary Tests Statistic Critical Skew Kurt KolmogorovD Tet indicates non-nomais dstribution p <0.01) 1.04293382 1.035 440063305 0.2196436 BarletCs Test indicates equal variances (p 0.07) 10.2440315 15.0S63171 Hypothesis Test (-tail,9.05)

NOEC LOEC ChV TU MSDu

&ISDp MSO MSE F-Prob df DunnetrsTest 1000 1200 1095.44512 0.1 2.62127616 0.10120757 661.916667 6.57037037 4.1E-26 5.54 Treatments vs D-Control 06-04-03Xix

Environmental Testing Solutions, LLC t-Page 5 of 6 4-Species: Ceriodaphnia dubia CdNaCLCR #:

ll I

Daily Chemistry:

Y Day I

Concentration Parameter 0

I 1

I 2

.1 4

I 1H (S.U.)

I +-Si I I.R.L I 1R'

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DO (mgzL) 1 I

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T8

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I

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4

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IA (o3 tr l

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Environmental Testing Solutions, LLC Species: Ceriodaphnia dubid n

I Parameter 3

Page 6 of 6 CdNaCLCR #:

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a I

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_ (OC) l_

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( t )

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zY

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PERMIlTEE NAME/ADDRESS ncde Faclt Nam4ocabn # Diffefent)

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423__843__6700_03__07___4 Site Vice President acate. wnd campe I un aw tat te are significant penaltis tar submitting alse SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED information, Includi th possibility of fri and kipsonmnt fhr knowing vioations.

OFFICER OR AUTHORIZED AGENT A

NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all atachments here)

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Previous editions may be used Page of

PERMITTEE NAMEJADDRESS fdcude Fadlit NameLocatn if Diffeent Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. Bx 2 000 ________

SODDY - DAISY TN 37384 fadWj TVA SEQUOYAH NUCLEAR PLANT 1ioLrL iAMILTONCOUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT DMR)

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L TN026450 107 G I F-FINAL I

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OFFICER OR AUTHORIZED AGENT AR NUMBER YEAR MO DAY TYPED OR PRINTED I

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COMMENTS AND EXPLANATION OF ANY VIOLATIONS Reference al aftachments herc)

No phosphorous bearing cleaning solutions were used. No Discharge this Period EPA Forin 3320-1 (REV 3199)

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PERMITTEE NAME/ADDRESS (incde FeilltyNamraoocaton f Different)

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Persons who maage ym or tise persowsdily r "bleu for Acting Environmental Supervisor 423 843-6700 03 07 14 Site Vice PresidentInformalion.

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423__843__6700_03__07___4 Site Vice President acbate, and complts. I sm we tt *there signiiw penlties for Wyftfing fais SIGNATURE OF PRINCIPAL EXECUTIVE TYPED_____________________

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Ptevious editions may be used Page I of 1

PERMITTEE NAMEIADDRESS (indude Fec Namtocston if ifernt Ns-

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COMMENTS AND EXPLANATION OF ANY VOLATIONS (Rebecea8ftaftadhrm~nishens)

Operations performs visual inspetions for floating debris and oil and grease during all backwashes.

.~~~~~~~~~~~~~~~~~~

____4 EPA Form 3320-1 REV 3199)

Pievious editions may be ed Page I o 1

PERMITTEE NAME/ADDRESS gInch/d. Fcfl/f Nameocaton ffDifrenO Name

.TVA - SEQUOYAH NUCLEAR PLANT bgdass P0. BOX 2000 SODOY -DAISY _JN 37384..

fi&jj-y JYA. SEQUOYAH NUCLEAR PLANT 120159n _AMILL.OC2Uff Y NAllONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM NPDES)

MAJOR DISCHARGEMONITORING REPORT DMRI (SUBR t)

I TN0026450

! l 117 G I F-FINAL I

PERMiT NUMBER I DISCHARGE NUMBER BACIWASH I--

l MQNIT;RI~n 'ERICIP iEFFLUENT IYEAR MO I AY IYEAR MO DAY mr_

.c, Form Approved.

OMB No. 2040-0004 r^rAn.Cr i ATTN: Stephanie A. Howard Froml 03 1 06 I 01 I Tol 03 06 l 30 1

PJW 1 n l

NOTE: Read Instructions before completinm this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY)

SAMPLE 0

9A 0

1/30 VISUAL MEASUREMENT 01345 1

0 0

PPI.

f"f*4A~~*~

f~

7 PASS=O EFFLUENT GROSS VALUE

'M O__

1 M

OIL AND GREASE VISUAL SAMPLE 0

94

(

1/30 VISUAL MEASUREMENT 84066 1

0 0

~PR YESuI=1 EFFLUENT GROSS VALUE

_N__

SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE~ ~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~-,~

MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law tha this doaiment and al attasdments were prepared under y l I TELEPHONE DATE itirdion Or suISIon In Wordane Wth a syStel desined to an tVa qualified personne fl\\,7_.

Richard T. Purcell propety gather and evalusae the Iformation submitted. Based on my khqukry of Psonor W

I pe*ons who mangtho system or those persons directly responsible fo gatherng Acting Environmental Supervisor 423 843-8700 03 07 14 Site Vice Presidentinfonrmation. the kfnnallon submitted Is. to the best of my kno~sdge end belief. Wue, 423___843___700__03__07__14 Site Vice President accuate, and complete. I sm swn th thee a significant penalties for submn false SIGNATURE OF PRINCIPAL EXECUTIVEI TYPED__________________

OR PRormsmaton.

Including the possibility of fine ndsonm" for knowi g violations.

OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED I

________________________________________CODE j

j j COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Operations performs visual inspections for floating debris and oil and grease during aN backwashes.

EPA Formn 3320-1 (REV 3199)

Previous editions may be used Page of

PERMITTEE NAME/ADDRESS tlchude FaciltvNanmocation ifDlffernt)

Namrte TVA SEQUOYAH NUCLEAR PLANT Addres P._O.

BOX 2000 ____________

(I"HE3OFF CE 58

-2 SODDY* AIS_ _jt37L8..

fqd!'._ _aA jEQUOYAH NUCLEAR PANT_...

Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR Form Approved.

DISCHARGE MONITORING REPORT DMR)

(SUBR 01)

OMB No. 2040-0004 l

TNOO26450

. l I 118 G l F-FINAL I

PERMIT NUMBER I [ DISCHRGENM WASTEWATER & STORM WATER AONKITOR*IMG 0FIODn 1 EFFLUENT YEAR YEAR oMO From 03 06 1 01 To 03 06 30 a

NO DISCHARGE [

i NOTF-Roas Instnidlnna hafm enmntatina this fnrm-ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FRE0UENC SAMPLE

_ _ _ _ _EX OF T Y PE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)

SAMPLE MEASUREMENT

~~~~~~~~~Evn 9117FFLUENT GROSS inW

_ MGL E

m Gm

00300, 1OA 0UPNE 0ESRMN 0

19 EFFLUENT GROSS VALUE M

m m

SOLIDS. TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 19 00530 1

0 0

MGIL 7

m 7

m EFFLUENT GROSS VALUE M

T(AI SOLIDS. SETTLEABLE SAMPLE 25 MEASUREMENT 00545 1

0 0

WMIT MEASUREMEN

c~

EFFLUENT GROSS VALUE

,.~~AL:X FLOW, IN CONDUIT OR THRU SAMPLE

e**

TREATMENT PLANT MEASUREMENT 03 50050 1

0 0

.MGD g

VCqM EFFLUENT GROSS VALUE

'4 O P&

3/4 iLiM I !

Ž f SAMPLE MEASUREMENT SAMPLE SAMPLE MEASUREMENT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Caerify -W pdally of lw that ts docet mid l attents we prepared w I

TELEPHONE DATE dlricton or superision In cordonce with a system desgnd to sse that quaified pTELEPH NEDAATE Richard T. Purcell pptgatr aid evate the inormation rihmitted. Based n my nqkiry of the person or persons wh~o mansg the syste or those personadiredly esponsible for galeing the Acting Environmental Supervisor 423 843-8700 03 07 14 Site Vice Presidentinformation, te information atibmitled is, to Dve best of my knowledge and belief,. e,_____________

Site Vice President u

&W comlte., am awbe that thn ea sigficant penafties for stibmitting false SIGNATURE OF PRINCIPAL EXECUTIE TYPED OR PRINTED Wa_________

information, Including the possibility of fine and imprisonment for kowtng violations.

OFFICER OR AUTHORIZED AGENTI AE NUMBER YEAR MO DA TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference a atachments hers)

During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

EPA Fonn 3320-1 (REV 399)

I:Vevious editions may be used Page of

ML032040289

Contents

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ML032040289

Text

Dear Mr. Hannah:

SUMMARY

Contact:

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