L-2014-150, Florida Power & Light Company Biological Plan of Study Implementation for St. Lucie Plant EPU Report of Preliminary Findings, August 2011 - January 2014

Florida Power & Light Company Biological Plan of Study Implementation for St. Lucie Plant EPU Report of Preliminary Findings, August 2011 - January 2014

ML14163A505
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Site:	Saint Lucie
Issue date:	05/31/2014
From:	Ecological Associates
To:	Florida Power & Light Co, Office of Nuclear Reactor Regulation
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ML14163A503	List: L-2014-150, Florida Power & Light Company Biological Plan of Study Implementation for St. Lucie Plant EPU Report of Preliminary Findings, August 2011 - January 2014 L-2014-150, St. Lucie, Units 1 and 2 - Environmental Protection Plan Report, Biological Plan of Study Implementation for St. Lucie Plant EPU, Report of Preliminary Findings ML14163A504
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L-2014-150
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Florida Power & Light Company Biological Plan of Study Implementation for St. Lucie Plant EPU Report of Preliminary Findings August 2011 - January 2014 Submitted to Florida Power & Light Company 700 Universe Blvd.

Juno Beach, FL 33408 May 2014 Preparedby Ecological Associates, Inc.

Post Office Box 405 Jensen Beach, Florida i CAL AS, i.

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY TABLE OF CONTENTS EXECUTIVE SUM M ARY .................................................................................................. ii INTRODUCTION ..................................................................................................................... I M ETHODS ................................................................................................................................ 2 Sampling Period..................................................................................................................... 2 GeneralSampling Areas ................................................................................................ 2 Specific Sampling Locations ............................................................................................. 3 Water Quality ......................................................................................................................... 3 Trawling ................................................................................................................................. 3 Gill Netting ............................................................................................................................. 4 Beach Seining ........................................................................................................................ 5 PlanktonNetting .................................................................................................................... 5 NearshoreH ardbottom Utilization by Sea Turtles............................................................ 5 Representative ImportantSpecies..................................................................................... 6 StatisticalAnalysis .................................................................................................................. 8 RESULTS .................................................................................................................................. 9 Water Quality ......................................................................................................................... 9 Dissolved Oxygen ............................................................................................................... 9 p H ..................................................................................................................................... 10 S a lin ity .............................................................................................................................. 11 Specific Conductivity ........................................................................................................ 11 Temperature ...................................................................................................................... 12 Trawling ............................................................................................................................... 13 Gill Netting ........................................................................................................................... 16 Beach Seining ...................................................................................................................... 18 Plankton Netting .................................................................................................................. 19 NearshoreHardbottom Utilization by Sea Turtles.......................................................... 21 DISCUSSION .......................................................................................................................... 21 REFERENCES ........................................................................................................................ 23 ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA i

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY EXECUTIVE

SUMMARY

An Extended Power Uprate (EPU) was undertaken to increase the generating capacity and improve operating efficiency of both units at Florida Power & Light Company's (FPL's) St.

Lucie Plant on Hutchinson Island, St. Lucie County, Florida. The EPU is expected to result in a slight increase (2.0°F) in the temperature of cooling water discharges into the Atlantic Ocean. A Biological Plan of Study (POS) was developed to assess the extent, if any, to which these elevated temperatures may affect the protection and propagation of a balanced, indigenous population of shellfish, fish and wildlife in the receiving water body. The Biological POS was approved by the Florida Department of Environmental Protection (FDEP) on August 18, 2011, and study implementation began soon thereafter. This report summarizes results of eight baseline monitoring events and six post uprate monitoring events conducted through January 2014.

Sampling consisted of water quality monitoring, collection of fish and shellfish by trawl, gill net, and beach seine, and monitoring of sea turtle utilization of nearshore hardbottom. Monitoring was performed approximately every other month between August 2011 and January 2014. Three discrete areas, one centered around the plant within the zone of thermal influence (Area SL2) and two reference sites located north (Area SLI) and south (Area SL3) of the plant, were used to establish baseline conditions.

Though there were a number of differences among areas with respect to monitored water quality variables, only rarely did Area SL2 differ significantly from both reference sites. There were no persistent trends indicating major differences in bottom or surface water column dissolved oxygen, pH, salinity, specific conductivity, or temperature among areas prior to or after the uprate.

Results of trawl sampling indicate a diverse invertebrate and fish fauna in all three areas with considerable spatial and temporal variability in faunal densities. Clupeiform fish (anchovies, herrings, and sardines), a Representative Important Species (RIS), accounted for over 40 percent of the total catch. The species comprising this group provide forage food for a large variety of predatory fish, many of which are commercially and recreationally important. Twelve Commercially/Recreationally Important (CRI) decapod crustaceans were collected in trawls during baseline sampling. There were no statistically significant differences in the mean number fish, RIS, or CRI decapod crustacean taxa among areas before or after the uprate. Furthermore, there were no statistically significant differences among areas pre- or post-uprate with respect to the mean Catch per Unit Effort (CPUE) for these groups or for individual RIS taxa. Finally, no statistically significant differences were detected between Area SL2 and the other two areas in the mean biomass of RIS captured before or after the uprate.

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PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY Gill-net sampling targeted fish including RIS. Atlantic bumper, Atlantic sharpnose shark, and bonnethead shark collectively accounted for over half of all captures. The most abundant RIS included Atlantic croaker and Atlantic Spanish mackerel. As with trawl sampling, there was considerable variability in the number of taxa and CPUE per sampling event. There were no statistically significant differences in the number of fish taxa among areas prior to the uprate.

After the uprate, statistical analysis indicated that the number of fish taxa in Area SL2 was significantly less than the number in SLI, but not significantly different from the number in Area SL3. There were no statistically significant differences in the number of RIS fish taxa among areas during either pre- or post-uprate sampling periods. For fish and RIS CPUE, there were no statistically significant differences among areas prior to or after the uprate. With regard to mean CPUE of individual taxa of RIS, only one of eleven taxa exhibited any statistically significant differences among areas. During post-uprate monitoring, the mean CPUE of clupeiformes was statistically significantly higher in the north reference site than the south reference site, however, the mean CPUE at the power plant site was not statistically significantly different from means in either other area. There were no statistically significant differences in mean RIS biomass among areas either before or after the uprate.

Beach-seine sampling provided information on fish (including RIS) inhabiting the surf zone.

Again, there was considerable variability among sampling events with respect to the number of taxa and, in particular, the number of individuals captured by seine. This was mainly due to a large catch of scaled sardines in Area SL3 during one sampling event. Aside from the large catch of scaled sardines, which accounted for 74 percent of all specimens collected, sand drum, Atlantic bumper, and Spanish sardine accounted for the largest number of individuals captured by seine. There were no statistically significant differences in numbers of fish or RIS taxa collected by beach seine among areas before or after the uprate. There were no statistically significant differences in the mean numbers of RIS among areas during pre- or post-uprate events when scaled sardines were included. When scaled sardines were excluded, there were no significant differences among areas during pre-uprate events, but during post-uprate events Area SL3 had a statistically significantly higher mean number of RIS fish than the Areas SLI and SL2. However, the post-uprate means for Areas SLI and SL2 were not statistically significantly different. When numbers of individual taxa of RIS were compared among areas, no statistically significant differences were found among areas during either pre- or post-uprate events. There were no statistically significant differences in RIS biomass among areas during either pre- or post-uprate events, with or without the inclusion of scaled sardines.

Numerous taxa of fish and CR1 crustacean larvae and large quantities of fish eggs were collected during plankton sampling. Clupeiformes dominated the larval fish, accounting for 47 percent of all individuals captured. As with other biological sampling, there was considerable variability among sampling events. However, differences in the mean number of fish larvae taxa, fish egg taxa, CRI crustacean taxa, fish larvae CPUE, fish egg CPUE, and CRI crustacean larvae CPUE ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 221

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY from plankton samples were not statistically significant among areas either before or after the uprate.

The mean number of green turtle sightings in Area SL2 was statistically significantly greater than the means in the other two areas during pre-uprate events, but not significantly different from those areas during post-uprate events. This change in the relationship among areas appeared to be due to slight increases in the reference sites and a small decrease at the discharge site between pre- and post-uprate events. None of these changes in mean numbers of green turtle sightings between pre- and post-uprate events within areas were statistically significant.

Whether the change in the relationship among areas reflects an uprate effect or simply natural variability in the distribution of green turtles should be revealed by subsequent monitoring.

Collectively, data collected to date indicate a diverse fish and shellfish community in nearshore waters of the Atlantic Ocean offshore the St. Lucie Plant. These faunal communities exhibit considerable spatial and temporal variability. Typically, faunal conditions in Area SL2 (discharge site) were not statistically significantly different from conditions found in the two reference sites during pre- or post-uprate monitoring. Additional post-uprate monitoring will allow for better comparisons of faunal conditions before and after the uprate.

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PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY INTRODUCTION Florida Power & Light Company (FPL) applied to the Florida Department of Environmental Protection (FDEP) for a revision to its National Pollution Discharge Elimination System (NPDES) Permit (FL0002208), which authorizes thermal discharges into the Atlantic Ocean from the St. Lucie Nuclear Plant on Hutchinson Island, St. Lucie County, Florida. The revision, which became effective on December 23, 2010, allows for a minor increase in effluent temperatures, approximately 2.0°F (1.1°C) under normal operating conditions, resulting firom an extended power uprate (EPU) for both Units I and 2 at the plant.

Administrative Order (AO) AO022TL authorizes the above-referenced St. Lucie Plant EPU with conditions. Paragraph 20 of the AO stipulates that no later than 90 days after the effective date of the AO, FPL shall prepare and submit for the Department's review and approval a Biological Plan of Study (POS). The AO further identifies specific elements to be incorporated into the design of the POS. These elements were derived from historical Environmental Protection Agency (EPA) guidance addressing the regulation of thermal effects associated with nuclear plants.

A draft Biological POS was prepared in early 2011 and discussions with FDEP ensued.

Following receipt of review comments in May 2011, a final Plan was submitted to the Department on June 1,2011 and approval was granted on August 18, 2011.

The Biological POS for the St. Lucie Plant EPU is intended to characterize the fish and shellfish fauna in the vicinity of the plant, and assess the extent, if any, to which the EPU has affected those communities. Based on the nature of the receiving water body (Atlantic Ocean - open waters), the small change in discharge temperature resulting from the EPU, the effectiveness of the offshore discharge pipes in diffusing heated cooling water, and the limited spatial area historically affected by discharges, effects of the EPU are anticipated to be limited. Results of the Biological POS coupled with extensive prior 316(a) studies at the St. Lucie Plant will enable FPL to effectively demonstrate whether or not the St. Lucie Plant EPU is jeopardizing the protection and propagation of a balanced, indigenous population of shellfish, fish and wildlife in the receiving water body.

As described below, the Biological POS consists of trawling, gill netting, beach seining, plankton netting, and monitoring of sea turtle utilization of nearshore hardbottom. Sampling techniques largely replicate those used in prior 316(a) demonstration studies at the St. Lucie Plant to provide comparable contemporary data. The POS called for baseline monitoring prior to completion of the St. Lucie Plant EPU, and two years of post-EPU monitoring. Baseline ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY sampling was conducted from August 2011 through October 2012, and the first year of post-uprate sampling from January 2013 through January 2014. The results of individual sampling events were previously reported to FPL. This report summarizes those results and compares pre-and post-uprate data between seasons and among study areas.

METHODS Sampling Period Baseline monitoring commenced in August 2011 following FDEP approval of the Biological POS and continued through October 2012. The EPU was completed in December 2012 and the first post-uprate sampling event was conducted in January 2013. Sampling was performed every other month for a total of eight baseline sampling events and six post-uprate events included in this report. For purposes of this report, the summer season encompasses sampling events conducted from May through October, and winter season from November through April.

General Sampling Areas Three separate sampling sites were established within the study area, each measuring approximately 2.0 mi (3.2 kin) on a side (Figure 1). Within the near and far field area surrounding St. Lucie Plant's discharge structures, the first site (SL2) extends from shore along the axis of the Unit 2 multi-port diffuser. The north and south boundaries of the discharge site are located 1 mi (1.6 kin) from the discharge structure and parallel the center line. Within this bounded area, three unique habitat types are present, each with a unique benthic and fish fauna:

the beach terrace (shallow sandy areas near shore in depths less than about 20 ft (6.1 m), an offshore trough (a relatively homogenous shell hash substrate in 35-40 ft (11-12 m), and an offshore shoal (sandy substrate that rises to a depth of approximately 20 ft (6.1 m; EAI, 2001).

Two additional sites of equivalent size to the discharge site and positioned similarly served as reference sites to document background conditions in areas unaffected by thermal discharges.

One of these was located approximately midway between the discharge site and the Ft. Pierce Inlet (SLI) and the other midway between the discharge site and the St. Lucie Inlet (SL3; Figure 1).

Precise sampling locations within each area were established during the initial baseline sampling event. A Global Positioning System (GPS) was used to capture sampling points and transect end points, as applicable. Following the initial sampling event, all sampling locations remained fixed for the duration of the study period.

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PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY Specific Sampling Locations Within each of the three areas, three transects progressively distant from shore were established for gill net and trawl sampling. The transects are located approximately 600 ft (183 m) from shore on the beach terrace in water depths of 8-32 ft (2.4-9.8 m; Transect A), approximately 4,000 ft. (1.2 kin) from shore in water depths of 34-45 ft (10.4-13.7 m; Transect B), and approximately 8,000 ft. (2.4 kin) from shore in water depths of 31-47 ft (9.4-14.3 m: Transect C).

A schematic of transect locations within the discharge area is shown in Figure 2. Plankton samples were collected concurrently with trawl samples in all three areas but only on Transects A and C.

Beach seining was conducted at three stations within each of the three sampling areas. Station A is located approximately 0.5 mi. (0.8 kin) north of the center of each area, Station B is located at the center of the area, and Station C is located approximately 0.5 mi. south of the center of the area (Figure 2). Final station locations were adjusted, as necessary, to avoid potential bottom obstructions.

For the purpose of boat-based sea turtle surveys, a 0.6 mi- (1 kin-) long transect was established in the vicinity of nearshore hardbottom in each of the three sampling areas. The approximate location of these transects was based on recent aerial photography and then adjusted in the field using towed underwater video cameras.

Water Quality Water quality data were recorded at three locations (middle and both end points) and three depths (approximately 30 cm below the.surface, mid-depth and 30 cm above the bottom) along each of the nine transects established for trawl and gill net sampling. A Hach Quanta water quality meter was used to document water quality at each sampling point. Monitored variables included: specific conductivity (mS/cm), temperature (°C), salinity as a function of conductance (practical salinity units [PSU]), dissolved oxygen (mg/L), and pH. The meter was calibrated prior to the start of daily sampling activities and again at the completion of sampling.

Trawling Trawling was conducted along each of the three shore-parallel transects established within each area, yielding a total of 72 baseline and 54 post uprate samples. Trawl sampling utilized a 16-ft (4.9-m) by 3-ft (0.9 in) semi-balloon bottom trawl, having a 0.5-in (12.7 mm) stretch mesh in the bag and 0.25-in (6.4-num) stretch mesh in the cod end. The net was towed on the bottom at speeds of 2-3 knots for 15 minutes along each transect. The tows typically intersected the ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 3

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY approximate midpoint of each of the three gill net transects (Figure 2). Sampling at all sites was performed at night.

All fish captured by trawl were identified to species and counted. Additionally, a maximum of 25 representative specimens of each Representative Important Species (RIS; see below) captured were measured (standard length and total length) and a batch weight for those specimens obtained. Trawl data are presented as total number of individuals captured, number of individuals captured per kilometer of bottom trawled (Catch per Unit Effort; CPUE), and average length and weight of RIS. CPUE is used to account for differences in tow distances resulting from varying boat speeds and currents. Biomass for RIS was determined by dividing the batch weight of each taxon by the number of specimens weighed and then multiplying the average weight by the total number of specimens in the sample.

All invertebrates captured by trawl were counted and identified to major taxonomic group (e.g.,

spider crab, swimming crab, squid, etc.). Additionally, any Commercially/Recreationally Important Species (CRI; e.g., blue crabs, penaeid shrimp, lobster, etc.) in the catch were identified to the lowest practicable taxon.

Gill Netting Gill net sampling was conducted along three shore-perpendicular transects established within each area, yielding a total of 72 baseline and 54 post uprate samples. Within the discharge study site, the transects were sited either north or south of the multi-port diffuser, depending on predominant current flow at the time of sampling, to ensure the net fished within the thermal plume, if present (Figure 2). The gill net was 600 ft (183 in) in length and 12 ft (3.7 m) in depth, and consisted of 5 monofilament mesh panels, each 120 ft (66.6 m) long. Mesh sizes (stretch length) of the five panels are as follows: 2.5 in (64 mm), 2.9 in (74 mm), 3.3 in (84 mm), 3.8 in (97 mm), and 4.6 in (117 mm). The variable mesh allows the capture of numerous fish species of different size classes. The bottom line of the gill net was weighted such that it rested on the bottom, and floats on the top line kept the net suspended in the water column. Sampling at all sites was conducted during daylight hours.

Gill nets remained in the water a minimum of 30 minutes, with total soak time calculated from the time the lead end of the net went into the water until the time the terminal end was back onboard the vessel. All fish captured by gill net were identified to species and counted.

Additionally, a maximum of 25 representative specimens of each RIS captured were measured (total length) and a batch weight for those specimens obtained. Gill net data is presented as described above for trawling with the exception that CPUE represents the number of individuals captured per hour of soak time. Soak times often varied considerably in relation to sea conditions and/or the number of fish being captured.

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PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY Beach Seining Beach seining was conducted at three locations within each of the three study areas, yielding a total of 72 baseline and 54 post uprate samples. The seine consisted of a 100-ft (30.5-m) long by 6.0 ft (1.8-m) deep net having a stretch mesh of 1 inch (25 mm). The net is heavily weighted along the bottom and has extra floatation on the top so it maintains a vertical hanging position in the water column under surf conditions. The rolled net was carried out to a depth of approximately 4 ft (1.2 in), deployed parallel to shore and then pulled onto the beach with the ends perpendicular to shore. This created a U-shaped barrier, with fish funneled to the central part of the net as it was brought on shore. Sampling was performed during daylight hours.

Fish and invertebrates captured by seine were processed, as described above for gill netting and trawling. Data are presented as total number of individuals captured, with average length and weight also reported for RIS. Insofar as the exact area of bottom sampled by the seine could not be accurately determined, no CPUE data are presented.

Plankton Netting Nighttime sub-surface plankton tows were made concurrently with trawling along Transects A and C within each study area (Figure 2), yielding a total of 48 baseline and 36 post uprate samples. Sampling was conducted using paired bongo nets (20-cm diameter) having a 500 micron mesh. Each net was equipped with flow meters to allow determination of volume of water filtered. The nets were towed for 15 minutes, unless clogging required shorter tow times.

Once the nets were retrieved aboard the vessel, the contents from both cod ends were combined into a single sample and preserved in 10% buffered formalin for laboratory identification. In addition to quantifying ichthyoplankton in the bongo net collections, any captured CRI decapod crustacean larvae were identified and enumerated. CRI target species included, but were not limited to, penaeid shrimp, blue crabs, stone crabs, lobster, and mole crabs. Plankton data are presented as total number of individuals captured and number of individuals captured per 100 cubic meter of water filtered (CPUE).

NearshoreHardbottoni Utilization by Sea Turtles A single l-kin shore-parallel transect was monitored in each of the three study areas. Each transect was traversed two times during each sampling event with at least a 30 minute separation between the two passes. A total of 48 baseline event observations and 36 post-uprate observations were made. Sea turtle surveys were performed from a boat equipped with an elevated platform capable of holding two observers. As the boat traversed each transect at a slow and constant speed (4.0 knots or less), one observer would look to port side and the other to starboard side. Observers would record and identify to species, when possible, any turtle ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 5

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY observed surfacing within approximately 100 ft (30 m) of the transect centerline. Monitoring days were selected for optimal viewing capabilities (e.g., sunny with calm seas).

RepresentativeImportantSpecies Section 4.0 of the Interagency 316(a) Guidance Manual (EPA, 1977) defines Representative Important Species as having one or more of the following traits: commercially or recreationally valuable; threatened or endangered; critical to the structure and function of the ecosystem; and/or a necessary component of the food chain for the preceding species. RIS for the Biological POS were selected based on results of prior 316(a) demonstration studies at the St. Lucie Plant and included 11 taxa of fish and one species of sea turtle, as follows:

1. Atlantic croaker (Micropogonias undulatus) - This bottom associated species, which is part of the recreational fishery, was one of the most abundant species captured in both gill nets and trawls during prior studies at the plant. It spawns offshore and gravid females have been found throughout the year.

2. Spot (Leiostomus xanthurus) - This bottom associated species is a common species along the Atlantic coast and was one of the most abundant species captured in both gill nets and trawls during prior studies at the plant. Spawning occurs offshore during the late fall, winter, and early spring and peaks in December and January.

3. Sand Drum (Umbrina coroides) - This bottom associated species is a common species along the Atlantic coast and was one of the most abundant species captured in both gill nets and trawls during prior studies at the plant. Spawning occurs offshore during the late fall, winter, and early spring and peaks in December and January.

4. Pigfish (Orthopristis chiysoptera) - This bottom associated species is a common recreational species along the Atlantic coast and accounted for a large percentage of biomass for all species captured by trawl during prior studies at the plant. Spawning occurs offshore during the fall, winter, and spring.

5. Bluefish (Pomatomius saltatrix) - This conmm-ercially and recreationally important schooling predator regularly occurs in coastal waters of east Florida, although it is present in greatest numbers during the fall and winter. It was captured in large numbers by gill net during prior studies at the plant. Gravid females were present throughout the year, although spawning within the south Atlantic fisheries stock occurs primarily in early summer along the continental shelf between northern Florida and Cape Hatteras.

6. Silver Seatrout (Cynoscion nothus) - This important recreational species, occurs in the vicinity of the plant and was relatively common in gill net and trawl collections during ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 6

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY prior studies at the plant. Seatrout are bottom associated species that spawn offshore, and gravid females have been found throughout the year.

7. Kingfish/Whiting (Menticirrhus spp.) - Both the Gulf (M littoralis) and southern (M americanus) kingfish occur in coastal waters over sandy bottom and were routinely captured in ocean beach seines during previous studies at the plant. Both species are taken in the recreational fishery. They typically spawn in the ocean during spring, summer and fall.

8. Florida pompano (Thachinotus carolinus)- This species is a commercially important and recreationally popular coastal species often found in small to large schools along sandy beaches. Florida pompano were often captured in beach seines during previous studies at the plant. This species primarily spawns in the spring and summer.

9. Spanish mackerel (Scomberomorus inaculatus)- This schooling coastal species is both commercially and recreationally important in eastern Florida waters and was one of the most frequently caught species in gill netting operations conducted during previous studies at the plant. Although it occurs throughout much of the year, largest concentrations occur during the fall and winter, with gravid females being present primarily during the spring, summer, and fall. Spanish mackerel feed on a variety of Clupeiform fish (herrings and sardines) which are extremely abundant in the nearshore waters off Hutchinson Island.

10. Clupeiformes - This group includes a variety of anchovies, herrings and sardines, the primary source of food for most of the commercially important piscivorous fish species that migrate through the area. Clupeiformes were numerically prevalent in both trawl and gill net sampling previously conducted at the plant.

11. Leopard Searobin (Prionotus scitulus) - The leopard searobin is a benthic oriented fish that is common over sandy substrates. It was one of the most frequently captured species in trawl sampling during previous studies at the plant. Individuals in spawning condition were captured in winter and spring.

12. Green sea turtle (Chelonia mnydas) - The green sea turtle is federally listed as endangered.

Juveniles are found in inland estuaries where they occupy and feed on submerged aquatic vegetation. They are also regularly found in the ocean where they feed on algae that colonize exposed hardbottom and worm reef. Large numbers of juvenile green turtles occur in the ocean in the vicinity of the St. Lucie Plant, as evidenced by their routine entrapment in the plant's cooling water intake system.

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PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY StatisticalAnalysis All variables were statistically analyzed using STATISTICA 64, Version 11 software (Statsoft, Inc.). Data were first tested to determine if they met requisite requirements for parametric testing, including normality (Shapiro-Wilks Test) and homogeneity of variance (Levene's Test).

These tests revealed that the water quality data met both assumptions; however, the faunal data did not. Based on these results, non-parametric tests were used for all analyses of faunal data.

To detect the potential impact of the St. Lucie EPU during faunal sampling events, water quality variables were analyzed using Repeated Measures Analysis of Variance (ANOVA) to determine if statistically significant differences (p<0.05) existed among sampling events. If significant differences were detected, the Tukey Honest Significant Difference (HSD) Test was used to determine which mean values were significantly different.

Separate analyses ,Vere done for each type of faunal sampling: trawls, gill nets, beach seines, plankton tows, and sea turtle surveys. Data were analyzed separately for: all fish, RIS, fish larvae, fish eggs, CRI decapod crustaceans and CR1 decapod crustacean larvae. Each group was analyzed for differences in the number of taxa collected, total number of individuals captured, and/or CPUE. Biomass was also analyzed for RIS fish. All data were analyzed using a Kruskal-Wallis Analysis of Variance by Ranks Test (ANOVA) to determine if statistically significant differences (p<0.05) existed among transects, areas, season, and pre- and post-uprate events. If significant differences were detected, the Multiple Comparisons of Mean Ranks Post-hoc Test was used to determine which values were significantly different within the ANOVA. In certain instances, the ANOVA and the Multiple Comparisons Tests reported conflicting results. In these cases, Mann-Whitney U Tests were performed among areas (including the following comparisons SLI vs SL2, SL2 vs SL3 and SLI vs SL3) to determine which were significantly different from each other.

Differences among transects may be indicative of nearshore to offshore gradients, while differences among study areas may be indicative of shore-parallel gradients. Seasonal changes and/or long-term temporal patterns are assessed by analyzing for differences among sampling events. For the purpose or these analyses the sampling events were grouped into either summer or winter seasons. The summer season included events from May through October while winter events were from November through April.

During the study period, an abnormally large number of individuals of a single taxon were captured during a few sampling events at one or more stations. For these events, the data was analyzed with and without the large catch to improve the resolution of the statistical tests. Due to the high variability of faunal data, additional statistical tests were performed in a nested design that reduced variability in the data to enhance the detection of significant differences among ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 8

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY areas. For example, the test comparing areas among all sampling events can be improved by removing the seasonal and nearshore/offshore variability by structuring the test to only analyze the data among areas, within a transect, within a season, within pre- or post-uprate event period.

Despite the attempts to improve the resolution of the statistical test, no additional significant differences were found among study areas and hence the data is not presented in this report.

RESULTS Water Quality Differences in time of day, tidal stage, and weather conditions both between and during each sampling event create considerable spatial and temporal variability in water quality parameters.

Nevertheless, general characterizations can be made about similarities and differences in measured variables among study areas comparing summer and winter pre- and post-uprate values. Data is presented in relation to the associated sampling method: daytime bottom and surface water quality variables with gill netting and nighttime bottom and surface water quality variables with trawling/plankton tows.

Dissolved Oxygen Gill Netting - Mean bottom dissolved oxygen (DO) during daytime sampling ranged from 5.54 to 6.55 mg/L and averaged 6.26 mg/L for all areas and seasons combined (Table 1).

There were no statistically significant differences among areas during either pre- or post-uprate events, though mean bottom DO did show a statistically significant decrease within SL 2 between pre- and post-uprate events (Figure 4). Mean surface DO ranged from 5.70 to 6.74 mg/L and averaged 6.39 mg/L for all areas and seasons (Table 2). As with bottom DO there were no statistically significant differences in surface DO means among areas during pre- or post-uprate events (Figure 3). However, statistically significant decreases in mean surface DO values between pre- and post-uprate events were documented within Areas SL2 and SL3. Appendix Figures 1 and 2 show the relationship of the three areas with respect to mean surface and bottom DO values during each of the 14 sampling events. There were no consistent differences among areas before or after the uprate.

Trawling - Mean bottom DO during nighttime sampling ranged from 5.99 to 6.76 mg/L and averaged 6.35 mg/L for all areas and seasons combined (Table 3). There were no statistically significant differences in mean bottom DO values among areas during either pre- or post-uprate events or between pre- and post-uprate events within any area (Figure 6). Mean surface DO ranged from 5.93 to 6.73 mg/L and averaged 6.39 mg/L for all areas and seasons (Table 4). During pre-uprate events the mean surface DO in SLI was statistically significantly higher than in SL3, though there was no statistically significant difference between the mean in ECOLOGICAL AssoCIATES, INC., JENSEN BEACH, FLORIDA 9

PRELIMINARY FINDINGS, ST. LucIE PLANT EPU BIOLOGICAL PLAN OF STUDY SL2 and the means in the other two areas (Figure 5). There were no statistically significant differences among areas during post-uprate events. Though there was a statistically significant decrease in mean surface DO in SLI between pre- and post-uprate events, there no statistically significant differences between pre- and post-uprate events in SL2 or SL3. There were no consistent differences among areas before or after the uprate with respect to mean surface (Appendix Figure 3) or mean bottom (Appendix Figure 4) DO.

pH Gill Netting - During daytime sampling, mean bottom pH ranged from 7.87 to 8.25, with a mean value of 8.04 for all areas and seasons combined (Table 5). There were no statistically significant differences among areas during pre-uprate events (Figure 8). During post-uprate events, the mean in SL2 was statistically significantly lower than in SLI but not statistically significantly different than in SL3. Within areas, means in SLI and SL3 exhibited a statistically significant increase between pre- and post-uprate events, but there was no statistically significant difference between pre- and post-uprate means in SL2. Mean surface pH ranged from 7.87 to 8.22 and averaged 8.02 for all areas and seasons combined (Table 6). As with bottom pH, there were no statistically significant differences in mean surface pH values among areas during pre-uprate events and during post-uprate events the mean in SL2 was statistically significantly lower than the mean in SLI but not statistically significantly different than the mean in SL3 (Figure 7).

Results of comparisons of mean surface pH values between pre- and post-uprate events within each area also followed the same pattern as with mean bottom pH. There were statistically significant increases in means in SLI and SL3 between pre- and post-uprate events, but no statistically significant change in mean surface pH in SL2. The relationship of the three areas with respect to mean surface and bottom pH during each sampling event are shown in Appendix Figures 5 and 6. There were no consistent differences before or after the uprate.

Tr-awling- During nighttime sampling, mean bottom pH ranged from 8.00 to 8.12, with a mean value of 8.06 for all areas and seasons combined (Table 7). There were no statistically significant differences in mean bottom pH among areas before or after the uprate nor were there any statistically significant changes in the means between pre- and post-uprate events within any area (Figure 10). Mean surface pH for trawls ranged from 7.99 to 8.10 and averaged 8.05 for all areas and seasons combined (Table 8). As with bottom pH, there were no statistically significant differences in mean surface pH values among areas pre- or post-uprate and no statistically significant changes after the uprate with any area (Figure 9). Appendix Figures 7 and 8 show the relationship of the three areas with respect to mean surface and bottom pH values during each of the 14 sampling events. There were no consistent differences among areas before or after the uprate.

ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 10

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY Salinity Gill Netting- Mean bottom salinity during daytime sampling ranged from 35.87 to 36.37 PSU, with a mean value of 36.21 PSU for all areas and seasons combined (Table 9). There were no statistically significant differences in mean bottom salinity values among areas before or after the uprate or within areas between pre- and post-uprate events (Figure 12). Mean surface salinity ranged firom 35.89 to 36.31 PSU and averaged 36.16 PSU for all areas and seasons combined (Table 10). As with mean bottom salinity, there were no statistically significant differences in mean surface salinity values among areas prior to or after the uprate or within any area between pre- and post-uprate events (Figure 11). There were no consistent differences among areas with respect to mean surface (Appendix Figure 9) or mean bottom (Appendix Figure 10) salinity before or after the uprate.

Trawling - Mean bottom salinity during nighttime sampling ranged from 36.17 to 36.51 PSU, with a mean value of 36.33 PSU for all areas and seasons combined (Table 11). There were no statistically significant differences in mean bottom salinity values among areas before or after the uprate or within areas between pre- and post-uprate events (Figure 14). Mean surface salinity ranged from 36.15 to 36.55 PSU with a mean of 36.31 PSU for all areas and seasons (Table 12). As with mean bottom salinity, there were no statistically significant differences in mean surface salinity values among areas prior to or after the uprate or within any area between pre- and post-uprate events (Figure 13). The relationship of the three areas with respect to mean surface and bottom salinity during each sampling event are shown in Appendix Figures 11 and

12. There were no consistent differences before or after the uprate.

Specific Conductivity Gill Netting - Mean bottom specific conductivity during daytime sampling ranged from 54.23 to 55.03 mS/cm, with a mean value of 54.81 mS/cm for all areas and seasons combined (Table 13). There were no statistically significant differences in the means among areas before or after the uprate or within any area between pre- and post-uprate events (Figure 16). Mean surface specific conductivity ranged from 54.16 to 54.99 rnS/cm and averaged 54.67 mS/cm for all areas and seasons (Table 14). As with bottom conductivity, differences in mean surface conductivity values among areas were not statistically significant before or after the uprate (Figure 15). And there were no statistically significant differences between pre- and post-uprate means within any area. There were no consistent differences among areas before or after the uprate with respect to mean surface (Appendix Figure 13) or mean bottom (Appendix Figure 14) specific conductivity.

ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA I1I

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY Trawling - Mean bottom specific conductivity during nighttime sampling ranged from 54.69 to 55.29 mS/cm and averaged 54.94 mS/cm for all areas and seasons combined (Table 15).

There were no statistically significant differences in mean values among areas before or after the uprate and there were no statistically significant differences in the means between pre- and post-uprate events in any area (Figure 18). Mean surface specific conductivity ranged from 54.51 to 55.30 mS/cm and averaged 54.86 mS/cm for all areas and seasons (Table 16). As with mean bottom specific conductivity, there were no statistically significant differences among mean surface specific conductivity values among areas before or after the uprate (Figure 17).

Furthermore, there were no statistically significant differences in surface means between pre-and post-uprate events in any area. Appendix Figures 15 and 16 show the relationship of the three areas with respect to mean surface and bottom conductivity values during each of the 14 sampling events. There were no consistent differences among areas before or after the uprate.

Temperature Gill Netting - Mean bottom water temperatures during daytime sampling ranged from 21.50 to 25.46'C with a mean of 23.91°C for all areas and seasons combined (Table 17).

Though there were slight increases in mean bottom water temperatures between pre- and post-uprate events in all three areas, these increases were not statistically significant and there were no statistically significant differences in the means among areas before or after the uprate (Figure 20). Mean surface water temperatures for daytime sampling ranged from 21.84 to 27.14'C with a mean temperature of 24.84°C for all areas and seasons combined (Table 18). As with bottom water temperatures, slight increases in surface water temperatures occurred between pre- and post uprate events in all areas. However, as was found for bottom water temperatures, there were no statistically significant differences among areas before or after the uprate and the differences between pre- and post-uprate events within areas were not statistically significant (Figure 19).

There were no consistent differences among areas before or after the uprate with respect to mean surface (Appendix Figure 17) or mean bottom (Appendix Figure 18) water temperatures.

Trawling - Mean bottom water temperature during nighttime sampling ranged from 21.94 to 25.72°C and averaged 24.14'C for all areas and seasons combined (Table 19). There were no statistically significant differences in mean bottom water temperatures among areas before or after the uprate and there were no statistically significant differences in the means between pre- and post-uprate events within any area (Figure 22). Mean surface water temperature during nighttime sampling ranged from 22.21 to 26.55'C and averaged 24.78°C for all areas and seasons combined (Table 20). As with bottom water temperatures, there were no statistically significant differences in mean surface water temperatures among areas prior to or after the uprate and there were no statistically significant differences in the means between pre-and post-uprate events within any area (Figure 21). The relationship of the three areas with ECOLOGICAL AssocIATES, INC., JENsEN BEACH, FLORIDA 12

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY respect to mean surface and bottom water temperature during each sampling event are shown in Appendix Figures 19 and 20. There were no consistent differences before or after the uprate.

Trawling Table 21 provides the common and scientific names of all organisms captured for all sampling types combined during eight pre-uprate and six post-uprate biological sampling events. One hundred fifty three (153) taxa, representing 79 fish, 62 crustaceans, and 12 miscellaneous taxa, were captured by trawl (Table 22). A total of 4,854 organisms were identified and enumerated from the collection (1,655 fish, 2,561 crustaceans, and 638 other organisms, primarily echinoderms). Nine (9) of the 11 RIS were represented in the catch, and collectively they accounted for 63% of all fish specimens collected during baseline trawling. Clupeiformes (anchovies, herrings, and sardines), represented primarily by unidentified Engraulids and the Cuban anchovy (Anchoa cubana), accounted for over 40% of the total fish caught. The species comprising this group provide forage food for a large variety of predatory fish, many of which are commercially and recreationally important. The star drum (Stellifer lanceolatus), Atlantic bumper (Chloroscombrus chrysurus), tomtate (Haemuion aurolineatum), and sand drum (Umnbrina coroides) were also caught in relatively high numbers. Collectively, these six taxa accounted for approximately 70% of the total catch. There were no statistically significant differences among areas with respect to number of fish taxa, RIS taxa, or CRI invertebrate taxa prior to or after the uprate (Table 23). However, the number of fish taxa was statistically significantly lower during post-uprate monitoring in comparison with pre-uprate monitoring at Areas SL2 and SL3 (Table 24). The number of RIS taxa was also significantly lower during post-uprate trawling at Area SL3.

Of the fish taxa, Engraulidae and Anchoa cubana had the greatest CPUE (2.85/km and 2.84/km respectively), while over 75% of the other fish captured had a CPUE of 0.10 or less (Table 25).

The mean CPUE for all fish taxa combined ranged from 1.53/km in Area SL2 for post-uprate summer events to 74.43/km in Area SLI for pre-uprate summer events (Table 26). The much higher mean CPUE for all areas in the pre-uprate summer season is due to single event catches of relatively high numbers of clupeiforme fishes. Comparing the winter season pre- and post-uprate mean CPUE by area shows smaller differences. When the mean CPUE values for all fish taxa were compared among areas, no significant differences were found (Table 23). Large catches of anchovies, Engraulidae, were responsible for a substantial amount of variation in the data, therefore, it was removed from analysis to improve the resolution of statistical tests. Even after excluding anchovies, no significant differences were found among areas before or after the uprate (Table 23). Though there was a statistically significant decrease in fish CPUE and fish CPUE excluding Engraulidae after the uprate in SL2, this also occurred in SL3 (Table 24).

BEACH, FLORIDA 13 ECOLOGICAL ASSOCIATES, ECOLOGICAL INC., JENSEN AssocIATES, INC., JENsEN BEACH, FLORIDA 13

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY Insofar as Clupeiformes (an RIS) accounted for a large percentage of the total fish catch, seasonal patterns for RIS CPUE were similar to those for all fish species when RIS were analyzed separately (Table 27).

The anchovies, herrings and sardines comprising the Clupeiformes occur in large schools that are common throughout the study area. However, their chance occurrence at the time and location of sampling can skew catch numbers. For example, Clupeiform fish accounted for 82% of all RIS captured during baseline monitoring, and all but one of those was captured during a single sampling event (October 2012; Table 28). Seventy-three (73) percent and 25 percent, respectively, of all Clupeiformes were caught in Areas SLI and SL2, with only 2 percent caught in Area SL3 for all analyzed sampling events. Thus, it is not surprising that Area SLI had the greatest number of all RIS specimens captured (725), and SL3 had the fewest (105). The mean CPUE for each RIS species were compared among areas before and after the uprate, however, no statistically significant differences were found (Table 29). However, comparisons within each area revealed that Prionotus scitulus was significantly more abundant during the pre-uprate period than the post-uprate period in Areas SL2 and SL3 (Table 30).

When captures were adjusted to account for variable tow distances, CPUE for RIS ranged from 54.24/km in Area SLI for summer pre-uprate events to 0.87/km in Area SL3 for winter pre-uprate events (Table 31). Prior to the uprate regardless of season, mean RIS CPUE at SL2 was intermediate between means at SLI and SL3. Comparing post-uprate summer events, means decreased in all areas with the lowest mean occurring in SL2. For winter events, means increased slightly after the uprate and there was little difference among areas. There were no statistically significant differences in mean RIS CPUE among areas before or after the uprate regardless of whether Engraulidae was included or excluded (Table 23). Within areas, no statistically significant differences were found when RIS CPUE was compared between pre- and post-uprate periods (Tables 24).

The mean total length (TL) for each taxon of RIS collected by trawl during pre- and post-uprate sampling is presented in Table 32. The species with the largest mean TL included Atlantic croaker (Micropogonias undulates; 237.36 mm), pigfish (Orthopristischrysoptera; 230.0 mam),

and silver seatrout (Cynoscion nothus; 225.44 mm).

Despite representing the greatest number of specimens captured by trawl during baseline sampling, Clupeiformes represented only 18% of the RIS biomass. Mean weights and total biomass for RIS collected within each area during baseline trawl sampling, all events combined, are presented in Tables 33 and 34, respectively. For many of the RIS there is not paired data for pre- and post- or summer and winter events for the areas. For example, during the summer pre-and post- events in SL1, nine of the 12 taxa were only captured in either pre- or post-uprate events but not both. Similarly eight of the nine RIS collected during winter sampling in SLI ECOLOGICAL AssocIATES, INC., JENSEN BEACH, FLORIDA 14

PRELIMINARY FINDINGS, ST. LucIE PLANT EPU BIOLOGICAL PLAN OF STUDY were only represented in pre- or post-uprate events but not both. The mean weight was lower in all areas during post-uprate versus pre-uprate sampling for both summer and winter. Total biomass is tied to number of fish collected and mean biomass. Since there were eight pre-uprate sampling events and six post-uprate events, the total biomass will be biased for pre-uprate sampling. Mean biomass removes the bias for the difference in number of sampling events. Area SL2 had a lower mean biomass than SLI or SL3 during summer pre-uprate sampling but was higher than SLI during winter sampling. During post-uprate sampling, SL2 was again lower than both reference sites during the summer but higher than SL3 in the winter. When Engraulidae was included, there were no statistically significant differences in mean RIS fish biomass among areas prior to the uprate (Table 23). After the uprate, statistical tests indicated that mean RIS fish biomass was significantly higher in SLI than SL3, but SL2 was not significantly different from either reference site. When Engaulidae was excluded, there were no statistically significant differences among areas before or after the uprate. Within SLI, the difference in mean RIS Fish biomass between pre- and post-uprate periods was not statistically significant (Table 24). However, statistical analysis indicated that the pre-uprate means in SL2 and SL3 were significantly greater than the post-uprate means (Table 24). The results of the statistical tests were the same whether or not Engaulidae was included.

A total of 3,199 invertebrates represented by 74 taxa were collected by trawl during pre- and post-uprate sampling (Table 22). The largest total number of invertebrates were captured in Area SL2 (1770) and the fewest in Area SL3 (444; Table 35). Mean CPUE for all invertebrates combined ranged from 11.92/km in Area SL3 to 45.55/km in Area SL2. Means in all areas were higher during the pre-uprate period than during the post-uprate period. Area SL2 had a higher mean CPUE than the other two areas during the summer pre-uprate period, but was intermediate between the other two areas during the summer post-uprate period. For winter sampling events, the mean CPUE in SL2 was intermediate between the means in the other two areas during both the pre- and post-uprate periods.

Twelve CRI decapods crustaceans were collected in trawls. Four taxa, roughneck shrimp (Rirnapenaeus sp.), speckled swimming crabs (Arenaeus cribrarius), pink shrimp (Farfantepenaeusduorarun), and roughneck shrimp (Rimapenaeus constrictus) made up over 82% of all CRI crustaceans captured (Table 22). Mean CPUE for CRI invertebrates ranged from 0.80/km in Area SL3 (post-uprate winter events) to 6.84/km in Area SL3 (pre-uprate summer events; Table 36). Mean CPUE for CRI decapod crustaceans for Area SL2 was slightly lower than the means for the other two areas during the summer pre-uprate sampling period, but intermediate to the other two areas during the other three sampling periods. There were no statistically significant differences in mean CPUE among areas prior to or after the uprate or within any area comparing pre- and post-uprate monitoring periods (Table 23 and Table 24).

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PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY Gill Netting Eighty-nine (89) taxa, representing 76 fish, 6 crustaceans, and 7 miscellaneous taxa, were collected by gill net sampling (Table 37). A total of 4,580 organisms were identified and enumerated from the collection (4,461 fish, 34 crustaceans, 84 echinoderms, and 1 mollusc).

The 10 most abundant species accounted for 78% of the catch, with three species, Atlantic sharpnose shark (Rhizoprionodon terraeno'ae; 17.1%), Atlantic bumper (17.0%), and bonnethead shark (Sphyrna tiburo; 16.5.0%), accounting for over half of all captures. All II RIS of fish were captured by gill net, and collectively they accounted for 22% of all fish collected during the first 14 sampling events. Atlantic Spanish mackerel (Scomberomorus maculants) and Atlantic croaker (Micropogoniasundulatus) were the most abundant RIS.

There were no statistically significant differences in the number of fish taxa among areas prior to the uprate (Table 38). After the uprate, statistical analysis indicated that the number of fish taxa in Area SL2 was significantly less than the number in Area SLI, but not significantly different from the number in Area SL3. There were no statistically significant differences in the number of RIS fish taxa among areas during either pre- or post-uprate sampling periods. Within areas, there were no statistically significant differences in the number of fish taxa between pre- and post uprate sampling events in Areas SLI or SL2, though the number of pre-uprate fish taxa in SL3 was found to be significantly higher than the post-uprate number (Table 39). There were no statistically significant differences in the number of RIS fish taxa between pre- and post-uprate events in any area.

Atlantic sharpnose sharks had the highest CPUE of all fishes (6.72/hr) followed by Atlantic bumper (6.68/hr), bonnethead sharks (6.49/hr); Table 40). The mean CPUEs for all fishes caught by gill net in pre-uprate events ranged from 36.10/hour in SLI winter events to 63.73/hour in SL3 winter events (Table 41). For all areas, mean CPUE for post-uprate events were lower than for pre-uprate events. Though SL2 had lower mean CPUE values than either of the reference sites during both pre- and post-uprate summer events, SL2 had a greater mean CPUE than SLI during pre-uprate winter events and a higher mean than both reference sites during post-uprate winter events. There were no statistically significant differences in mean fish CPUE among areas during pre- or post-uprate events (Table 38). Within each area, there were no statistically significant differences in fish CPUE between pre- and post-uprate events (Table 39).

There was considerable fluctuation in the number of RIS specimens captured between pre- and post-uprate events in all areas, particularly during the summer (Table 42). Area SL2 was intermediate between the reference sites in pre-uprate summer seasons. During post-uprate summer events only two RIS specimens were collected in each of the reference sites and none were collected in SL2. For winter events, the number of RMS specimens in SL2 was lower than in either reference site during pre-uprate period but higher than either reference site during post-ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 16

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY uprate period. The relationships among areas described for the number of RIS specimens is the same as the relationship based on mean CPUE of RIS (Table 43). There were no statistically significant differences in RIS CPUE among areas before or after the uprate (Table 38).

Furthermore, there were no statistically significant differences in RIS CPUE between pre- and post-uprate events within any area (Table 39).

With regard to mean CPUE of individual taxa of RIS, only one of eleven taxa exhibited any statistically significant differences among areas. During post-uprate monitoring, the mean CPUE of clupeiformes was statistically significantly higher in Area SLI than Area SL3, however, the SL2 mean was not statistically significantly different from means in either other area (Table 44).

Though there were statistically significant decreases in mean CPUEs for two RIS taxa, Atlantic croaker (Micropogoniasundulatus) and bluefish (Pomatomus saltatrix), between pre-uprate and post-uprate events, those decreases were only observed in Areas SLI and SL3 (Table 45). There were no statistically significant differences in mean CPUEs of any RIS taxa between pre- and post-uprate events in SL2.

The mean total lengths (TL) for each taxon of RIS collected in gill net sampling are presented in Table 46. The species with the greatest mean TL included Atlantic Spanish mackerel (476.4 mm), bluefish (Pomatornus saltatrix; 409.4 mm), and Gulf kingfish (Menticirrhus littoralis; 377.7 mm).

Mean weights and total biomass for RIS collected within each area during gill netting are presented in Tables 47 and 48, respectively. Atlantic Spanish mackerel (Scomberomorus maculatus) accounted for 41% (173.0 kg) of the total biomass for all RIS collected in gill net sampling, with blue fish (Pomatoinus saltatrix; 67.3 kg), Atlantic croaker (Micropogonias undulates; 50.0 kg), and yellowfin menhaden (Brevoortia smithi; 41.3 kg) accounting for another 38%. Area SL2 total RIS biomass was intermediate to values in the two reference sites during summer pre-uprate events (Table 49). Post-uprate summer samples had very few RIS taxa captured in any area so biomass was very low in all areas. During winter events, total RIS biomass in SL2 was lower than in either reference site during the pre-uprate period, but higher than either reference site during the post-uprate period. There were no statistically significant differences in mean RIS biomass among areas before or after the uprate (Table 38) or between pre- and post-uprate events in any area (Table 39).

The only CRI invertebrate captured in gill net sampling was the speckled swimming crab, which was represented by only 15 specimens (Table 37). Too few were captured to allow for meaningful statistical analyses.

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PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY Beach Seining Fifty-five (55) taxa, representing 49 fish and 6 crustaceans, were collected during beach seine sampling (Table 50). For all events combined, a total of 26,722 organisms were identified and enumerated firom the collection (26,676 fish and 46 crustaceans). The scaled sardine (Harengula jaguana), was by far the most abundant species (19,726 individuals), accounting for 74% of all specimens collected. Three additional taxa, sand drum (Umbrina coroides), Atlantic bumper (Chloroscombrus chiysurus) and Spanish sardine (Sardinellaaurita), were each represented by more than 1,000 individuals, and collectively these four taxa accounted for 87% of all fish caught. Since beach seining does not have a method of normalizing the data using CPUE and there are unequal numbers of pre- and post-uprate events, numbers of individuals captured per event are used for comparisons (Table 51). Based on the mean number of fish captured per event, the value in SL2 was lower than the values in the other two areas during the summer pre-uprate events, but intermediate to the values in the other two areas during the summer post-uprate events (Table 52). During winter events, the mean number of fish per event was higher in SL2 than the other areas pre-uprate and lower than both those areas post-uprate. There were no statistically significant differences in mean numbers of fish or fish taxa among areas either prior to or after the uprate regardless of whether or not Harengulajaguanawas included (Table 53).

Furthermore, there was no statistically significant difference between pre- and post uprate means within any area (Table 53).

Eighteen taxa of RIS were among the fish captured by beach seine. Of these, Clupeiformes (represented by 11 species of sardines, herrings, and anchovies) was the most abundant, accounting for 90% of the total catch (Table 54). There were no statistically significant differences in the number of RIS taxa among areas or between pre- and post-uprate events within any area (Tables 53 and 55).

A large proportion of total RIS captures (78%) occurred during a single event (August 2012) in a single area (SL3), due primarily to large numbers of scaled sardines (Harengulajaguana).

Despite this large catch of scaled sardines, there were no statistically significant differences in the mean numbers of RIS among areas during pre- or post-uprate events (Table 53). When scaled sardines were excluded from statistical analyses, there were no significant differences among areas during pre-uprate events, but during post-uprate events SL3 had a significantly higher mean number of RIS fish than SLI and SL2. There was no statistically significant difference in the post-uprate means for SLI and SL2. There were no statistically significant differences within areas between pre- and post-uprate number of RIS fish (Table 55).

When numbers of individual taxa of RIS were compared among areas, no statistically significant differences were found among areas during either pre- or post-uprate events (Table 56). Also, ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 18

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY there were no statistically significant differences in mean numbers of any RIS taxa between pre-and post-uprate events within any area (Table 57).

The mean total lengths (TL) for each taxon of RIS collected by beach seine are presented in Table 58. The species with the greatest mean TL included Spanish mackerel (201.0 mm)

Atlantic croaker (199.3 mm), and Gulf kingfish (103.9 mm).

Total biomass for RIS collected within each area during baseline beach seining, are presented by area in Table 59. Scaled sardines accounted for 74% (160.1 kg) of the total RIS biomass. The addition of sand drum (25.3 kg), Gulf kingfish (15.1 kg), Spanish sardine (5.5 kg), and Florida pompano (Trachinotus carolinus;4.7 kg) elevate the combined percentage of total RIS biomnass to 98%. Among study areas, mean biomass ranged from 5.2 kg in Area SL2 to 382.0 kg in Area SL3 (Table 60). There were no statistically significant differences in RIS biomass among areas during either pre- or post-uprate events, with or without the inclusion of the single episodic event of scaled sardine (Table 53).

Five taxa of CR1 decapod crustaceans were captured during baseline beach seine sampling, the speckled swimming crab, three taxa of mole crabs (Albuneidae, Emerita sp., and Ernerita talpoida), and the spiny lobster (Panulirusargus) which collectively accounted for 46 specimens (Table 50). The mole crabs were only captured in Area SL3 during the pre-uprate period, but in all areas during the post-uprate period. Arenaeus cribrariuswas collected in all areas. There were too few specimens of CR! decapod crustaceans captured to allow for meaningful statistical analyses.

PlanktonNetting One hundred seventy-five (175) taxa of fish (140), crustaceans (25), and fish eggs (10), were collected by plankton nets (Table 61). A total of 58,083 organisms were identified and enumerated from the collection (5,138 fish, 8,176 crustaceans, and 44,769 fish eggs). Not all fish and fish eggs could be identified to species level, so RIS were not included in the statistical analysis. However, 2,410 fish larvae could be identified as Clupeiformes, which is a RIS, and it accounted for 47% of all fish larvae collected. Collectively, 8 taxa comprised over 75% of the ichthyoplankton captured during plankton tows, including members of the families Blennidae, Clupeidae, Engraulidae, Gobiidae, and Sciaenidae. All species of Clupeidae, as well as some taxa within the Sciaenidae are RIS.

All events combined, Area SL2 had an intermediate total number of larval fish captured (2079),

with SLI having the highest (2,168) and SL3 having the lowest (891, Table 62). The numbers of larval fish in SL2 were higher than in the other two areas during all but the pre-uprate winter events when the number was intermediate to the other two areas. Area SLI during pre-uprate ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 19

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY winter events had the largest number of larval fish collected by plankton netting, all taxa combined (1,260). This number is primarily due to a large number of Clupeidae larvae (Table 61).

The mean CPUE (number collected per 100 m3 of water filtered) for each taxon collected by plankton nets is presented in Table 63. For summer events, mean CPUE of all taxa (fish and CRI crustacean larvae and fish eggs) in SL2 was intermediate to the means for the other two areas prior to the uprate and greater than the means for the other two areas after the uprate (Table 64).

For winter events, mean CPUEs were similar among areas before and after the uprate.

For summer events, fish larvae CPUEs were higher in SL2 than the other two areas both before and after the uprate (Table 67). For winter events, fish larvae CPUE in SL2 was intermediate to the values in the other two areas prior to the uprate, but higher than the other two areas after the uprate. Differences in the mrean number of fish larvae taxa and mean fish larvae CPUE were not statistically significant among areas either before or after the uprate (Table 65). Furthermore, there were no statistically significant differences in the mean number of fish larvae taxa or mean fish larvae CPUE between pre- and post-uprate events within any area (Tables 66).

There was some variation in the relationship among areas with respect to mean CPUE for fish eggs, however, when all events were combined the mean for SL2 was greater than the means for the other two areas (Table 68). Differences in the mean number of fish egg taxa and CPUE among areas were not statistically significant before or after the uprate (Table 65). There were no statistically significant differences in fish egg CPUE between pre- and post-uprate events within any area (Table 66). Though there were no statistically significant differences in mean number of fish egg taxa between pre- and post-uprate events within either SLI or SL2, the mean within SL3 was statistically significantly higher during pre-uprate events (Table 66).

A relatively large number of CRI decapod crustacean larvae were captured during plankton collections, with five taxa comprising over 75% of the crustaceans captured, including the swimming crabs (Callinectes sp.), mole crabs (Albunea sp. and Emerita talpoida), blue crabs (Callinectes sapidus), and stone crabs (Menippe mercenaria) (Table 61). The mean CPUE for each CRI crustacean taxa captured by plankton nets is presented in Table 63. The mean CPUE for CRI crustacean larvae over all sampling events combined ranged from 11.00 in Area SLI to 15.26 in SL2 (Table 69). Differences in CRI crustacean larval taxa and CPUE among areas were not statistically significant among areas for any time period (Table 65). Furthermore, there were no statistically significant differences in CRI crustacean taxa or CPUE between pre- and post-uprate events within any area (Table 66).

ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 20

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY NearshoreHardbottom Utilization by Sea Turtles The mean number of green turtle sightings was consistently higher in Area SL2 than the other two areas during both pre-uprate and post-uprate sampling periods (Table 70). Combining data from all events, the average green turtle sightings per one-kilometer-long transect ranged fi'om 0.04 at SL3 to 2.54 at SL2. Statistical comparisons among areas indicated that the mean number of green turtle sightings at Area SL2 was significantly greater than the means at the other two areas during pre-uprate events, but not significantly different from those areas during post-uprate events (Table 71). This change in the relationship among areas appears to be due to slight increases in SLI and SL3 and a slight decrease in SL 2 between pre- and post-uprate events.

These changes in mean numbers of green turtle sightings between pre- and post-uprate events within areas were not statistically significant (Table 72).

DISCUSSION The purpose of baseline monitoring during implementation of the Biological POS was to establish background conditions against which to assess the effects of the St. Lucie Plant EPU.

Monitoring was not intended to document effects of prior plant operations, as results of previous 316(a) studies concluded that operation of the St. Lucie Plant was not jeopardizing the protection and propagation of a balanced, indigenous population of shellfish, fish and wildlife in receiving waters of the Atlantic Ocean. Rather, the Biological POS is designed solely to assess the effects of a minor, elevation in discharge water temperatures on faunal communities following completion of the EPU. This report summarizes the results through the first year of post-uprate monitoring and provides preliminary comparisons of water quality and biological variables before and after the uprate. An additional year of post-uprate monitoring will be conducted and a final report will summarize the results of the entire study period.

Collectively, data collected to date indicate a diverse fish and shellfish community in nearshore waters of the Atlantic Ocean offshore the St. Lucie Plant. These faunal communities as well as the monitored water quality parameters exhibit considerable spatial and temporal variability.

Results of analyses of water quality data indicated that there were no statistically significant differences among areas before or after the uprate with respect to temperature, salinity or specific gravity. In the case of pre-uprate nighttime surface DO, the mean for the northern reference site was found to be statistically significantly higher than the mean for the southern reference site but there were no statistically significant differences in means between the power plant area and either reference site. Furthermore, no statistically significant differences were found among areas for pre-uprate daytime surface DO or for either daytime or nighttime post-uprate DO values. Though post-uprate surface and bottom pH values were statistically significantly higher in the north reference site than the power plant site for daytime events, neither surface or bottom ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 21

PRELIMINARY FINDINGS, ST. LucIE PLANT EPU BIOLOGICAL PLAN OF STUDY means at the power plant site statistically significantly differed from corresponding means in the south reference site. Furthermore, during nighttime sampling there were no statistically significant differences in surface or bottom pH values among areas. Taking all results into account, there was no indication that water quality was affected by the uprate.

For trawl samples, there were no statistically significant differences in the number of fish taxa, RIS taxa, or CRI invertebrate taxa among areas before or after the uprate. Furthermore, there were no statistically significant differences among areas before or after the uprate with respect to fish, RIS, or CRI invertebrate CPUE or the CPUE of individual RIS taxa. When Engraulidae was included there were no statistically significant differences in mean RIS fish biomass among areas prior to the uprate. After the uprate, statistical tests indicated that mean RIS fish biomass was significantly higher in the north reference site than the south reference site, but the power plant site was not significantly different from either reference site. When Engaulidae was excluded, there were no statistically significant differences among areas before or after the uprate. Based on these results there is no indication that the uprate affected the fish, RIS, or CRI invertebrates collected by trawl.

With respect to gill net sampling, there were no statistically significant differences in the number of fish taxa among areas prior to the uprate. After the uprate, statistical analysis indicated that the number of fish taxa at the power plant site was significantly less than the number in the north reference site, but not significantly different from the number in the south reference site. There were no statistically significant differences in the number of R1S fish taxa among areas during either pre- or post-uprate sampling periods. For fish and RIS CPUE, there were no statistically significant differences among areas prior to or after the uprate. With regard to mean CPUE of individual taxa of RIS, only one of eleven taxa exhibited any' statistically significant differences among areas. During post-uprate monitoring, the mean CPUE of clupeiformes was statistically significantly higher in the north reference site than the south reference site, however, the mean CPUE at the power plant site was not statistically significantly different from means in either other area. There were no statistically significant differences in mean RIS biomass among areas either before or after the uprate. As with the results of trawl collection analyses, there is no indication of an uprate affect on fish or RIS collected by gill nets. Too few CR1 invertebrates were collected by gill net for any meaningful comparisons.

There were no statistically significant differences in numbers of fish or RJS taxa collected by beach seine among areas before or after the uprate. Furthmore, the differences in the mean numbers of fish collected was not statistically significant among areas prior to or after the uprate, regardless of whether scaled sardines (Harengulajaguana) were included or excluded. There were no statistically significant differences in the mean numbers of RIS among areas during pre-or post-uprate events when scaled sardines were included. When scaled sardines were excluded, there were no significant differences among areas during pre-uprate events, but during post-uprate events the south reference site had a statistically significantly higher mean number of RIS fish than the north reference site and the discharge site. The post-uprate means for the north ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 22

PRELIMINARY FINDINGS, ST. LUCIE PLANT EPU BIOLOGICAL PLAN OF STUDY reference site and the power plant site were not statistically significantly different. When numbers of individual taxa of RIS were compared among areas, no statistically significant differences were found among areas during either pre- or post-uprate events. There were no statistically significant differences in RIS biomass among areas during either pre- or post-uprate events, with or without the inclusion of scaled sardines. Collectively these results give no indication of an uprate affect on fish or RIS. Too few specimens of CRI invertebrates were collected by beach seine for any meaningful comparisons.

Differences in the mean number of fish larvae taxa, fish egg taxa, CRI invertebrate taxa, fish larvae CPUE, fish egg CPUE, and CRI invertebrate CPUE from plankton samples were not statistically significant among areas either before or after the uprate. These results provide no indication of an uprate effect on fish larvae, fish eggs, or CRI invertebrate larvae.

Statistical comparisons among areas indicated that the mean number of green turtle sightings at the power plant site was significantly greater than the means at the two reference sites during pre-uprate events, but not significantly different from those areas during post-uprate events. This change in the relationship among areas appeared to be due to slight increases in the reference sites and a small decrease at the power plant site between pre- and post-uprate events. None of these changes in mean numbers of green turtle sightings between pre- and post-uprate events within areas were statistically significant. Whether the change in the relationship among areas reflects an uprate effect or simply natural variability in the distribution of green turtles should be revealed by subsequent monitoring.

REFERENCES EA] (Ecological Associates, Inc.). 2001. Survey of Aquatic Environments Potentially Affected by the Operation of the St. Lucie Power Plant, Hutchinson Island, Florida. Prepared by Ecological Associates, Inc., Jensen Beach, Florida, for Florida Power & Light Company.

41 pp.

EPA (U.S. Environmental Protection Agency). 1977. Interagency 316(a) Technical Guidance Manual and Guide for Thermal Effects Sections of Nuclear Facilities Environmental Impact Statements. U.S. Environmental Protection Agency, Office of Water Enforcement, Permits Division, Industrial Permits Branch, Washington, D.C. 79 pp.

BEACH, FLORIDA 23 ECOLOGICAL INC., JENSEN ASSOCIATES, INC.,

ECOLOGiCAL ASSOCIATES, JENSEN BEACH, FLORIDA 23

0 2.5 5 10 I i t a I a a a I Kilometers 0

..,OWIW J

Atlantic Ocean a SL3 Hutchinson Island St. Lucie Inlet I=

Figure 1. Location of Three Study Sites for the Biological Plan of Study, FPL St. Lucie Plant EPU.

I

LEGEND 0 0.5 1 2 Kilometers

---

Discharge Pipes

,- Gill Net Transects Trawl &Bongo Net Transects Trawl Transects 0 Beach Seine Locations

'0 01 Atlantic Ocean Indian River Lagoon

-Hutchinson Island Figure 2. Location of the Cooling Water Discharge Pipes and Biological Sampling Locations at the Discharge Study Site, FPL St. Lucie Plant EPU.

Figure 3. Mean dissolved oxygen in surface water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for daytime gill netting events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means.

Data were compared using MANOVA (df = 2, n = 366, F = 1.8066, p = 0.1657).

6.8 6.7 L 6.6 L 6.5 [

6.4[

E 6.3 8

6,2 [

6.1 [

6.0

=- Area SLO 5.9 FArea SL2

=;;Area SL3 5.8 Pre Post U prate Figure 4. Mean dissolved oxygen in bottom water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for gill netting over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 3.8532, p = 0.0221).

6.7 6.6[

6.5 .

6.4 I 6.3 I 96.2 6.1 I 6.0 5.9

• Area SL1 5.8  : Area SL2 4

'EArea SL3 5.7 Pre Post Uprate

Figure 5. Mean dissolved oxygen in surface water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for night time trawling over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confitlence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 3.2009, p =

0.0419).

6.7, 6.6 -

6.5 L C) 6.4 .

E S 6.3 I 6.2 6.1 Area SLI

Y-Area SL2 E Area SL3 6.0 Pre Post Uprate Figure 6. Mean dissolved oxygen in bottom water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for night time trawling over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df = 2, n = 366, F = 0.7968, p

0.4516).

6.7 r 6.6 1 6.5 F 6.4 I E

0 6.3 I a

6.2 F 6.1 -:AreaSUi MAj'ea SL2 DEArea SU3 nilu Pre Post Uprate

Figure 7. Mean pH in surface water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means.

Data were compared using MANOVA (df= 2, n = 366, F = 3.3190, p = 0.0373).

8.25 r 8.20 L 8.15 [

8.10 I

-. 8.05 8.00 F 7.95 F 7.90 L I.-  :;5Area SUi

aArea SL2 7.85 HEArea SO Pre Post Uprate Figure 8. Mean pH in bottom water among sampling areas (all 3 transects combined) during pre-and post-uprate periods for daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013),

St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means.Data were compared using MANOVA (df= 2, n = 366, F = 4.97 10, p = 0.0074).

8.25 r 8.20 8.15 F 8.10 [

= 8.05 8.00 [

7.95 F

• Area SLU 7 .90[

5:Area SL2

ý-Area SL3 7.85 Pre Post Uprate I

Figure 9. Mean pH in surface water among sampling areas (all 3 transects combined) during pre-and post-uprate periods for night time trawling over 14 sampling events (Aug 2011 - Dec 2013),

St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 0.1360, p = 0.8729).

8.14 .

8.12 [

8.10 .

8.081 8.06 [

= 8.04

-L j

8.02 1 8.00 L 7.98 I 5Area SLU 7.96 =5-Area SL2

=ý;- Area SL3 7.94 Pre Post Uprate Figure 10. Mean pH in bottom water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for night time trawling over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means.

Data were compared using MANOVA (df= 2, n = 366, F = 0.2895, p = 0.7488).

8.18,.

8.16 I 8.14 [

8.12 F 8.10 [

8.08 L "I-0I.

8.06 1 8.04 F 8.02 F 8.00 :TArea SLU 7.98 :FArea SL2

ý--Area SL3 7.96 Pre Post Uprate

Figure 11. Mean salinity in surface water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for daytime gill netting over 14 sampling events (Aug 2011 -

Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 0.0428, p = 0.9581).

36.5, 36.4 36.3 .

W 36.2

=36.1 09 36.0 I

• Area SL1 35.9 1

B:Area SL2

.:EArea SL3 35.8 Pre Post Uprate Figure 12. Mean salinity in bottom water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for daytime gill netting over 14 sampling events (Aug 2011

- Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 0.9072, p = 0.4046).

36.5 r 36.4 .

36.31.

50 36.2[

U) 0.

36.1 .

36.0 EJ:Area SUi 35.91.

3EArea sL2 ajNea SL3 35.8 Pre Post Uprate

Figure 13. Mean salinity in surface water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for night time trawling over 14 sampling events (Aug 2011 -

Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 0.2681, p = 0.7650).

36.6 r 36.5 I 36.4 5.

C,)

0- 36.3 Co 36.2 .

36.1 . 3EArea SUi ETI:Aea SL2 EýArea SL3 36.0 Pre Post Uprate Figure 14. Mean salinity in bottom water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for night time trawling over 14 sampling events (Aug 2011 -

Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 0.4917, p = 0.6120).

36.6 r 36.5 36.4 [

Co 0- 36.3 .

CO 70 36.21[

36.1 ý EArea SLI

]FNea SL2 EENea SU3 36.0 Pre Post Uprate

Figure 15. Mean specific conductivity in surface water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 0.0148, p -

0.9854).

55.1 ,

55.0L 54.9 F 54.8 zE.,54.7 54.6 L 0

0 54.5 F I.

CO 54.3 I 6Area SL1 54.2 EArea SL2 5Area SL3 54.1 Pre Post Uprate Figure 16. Mean specific conductivity in bottom water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 0.1575, p =

0.8544).

55.3 r 55.2 I 55.1 CO, E

555.0 I.

C:

0 54.9 I at 54.8 I.

CO 54.7 I EEArea SL1

T-Area SL2

Z;*Area SL3 54.6 Pre Post U prate

Figure 17. Mean specific conductivity in surface water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for night time trawling over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df = 2, n = 366, F = 0.1801, p =

0.8353).

55.3 r 55.2 1 F.

E 55.0 "0 54.9 I.

S54.8 I.

C5 C/) 54.7 I.

=ý-Area SLO 54.6 .

5=Area SL2 Area SL3 54.5 Pre Post Uprate Figure 18. Mean specific conductivity in bottom water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for night time trawling over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df = 2, n = 366, F = 0.1575, p

0.8544).

55.3 55.2 I 55.1 CO E

55.0 I.

C.5 0o 54.9 0

I.

C. 54.8 L W,

54.7 . :6*Area SLU

Area SL2

-Area SL3 54.6 Pre Post Uprate

Figure 19. Mean temperature in surface water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for daytime gill netting over 14 sampling events (Aug 2011 -

Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 0.1194, p = 0.8875).

26.5 26.0 25.5 .

25.0 I E 24.5 I 4) 24.0 L 23.5 [  :;: Area SLU R Area SL2

=ý-Area SL3 23.0 Pre Post Uprate Figure 20. Mean temperature in bottom water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for daytime gill netting over 14 sampling events (Aug 2011 -

Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 0.6737, p = 0.5105).

25.0, 24.5 [

24.0 I 23.5 .

E i-O 23.0 I 22.5 [  ::*:Area SLU EArea SL2

-+/-F-Area SL3 22.0 Pre Post Uprate

Figure 21. Mean temperature in surface water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for night time trawling over 14 sampling events (Aug 2011 -

Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 1.7792, p = 0.1702).

25.4 25.21 25.0 I op 24.8 24.6 I 01.

E gi 24.4 IJ 24.2

T>-Area SLU 24.0 5::Area SL2

ý--Area SL3 23.8 Pre Post Uprate Figure 22. Mean temperature in bottom water among sampling areas (all 3 transects combined) during pre- and post-uprate periods for night time trawling over 14 sampling events (Aug 2011 -

Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Data were compared using MANOVA (df= 2, n = 366, F = 3.3921, p = 0.0347).

25.2 r 25.0 24.8 24.6 24.4

. 24.2

. 24.0 C.

E 23.8 IT 23.6 23.4 [

23.2 :5*Area SLU 23.0  ::UArea SL2

4--Area SL3 22.8 Pre Post Uprate

Table 1. Mean Bottom Dissolved Oxygen (mg/L) for Gill Net Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter Area Pre- Post- Pre- Post- Mean SLI 6.07 5.86 6.51 6.55 6.22 SL2 6.37 5.54 6.46 6.35 6.21 SL3 6.44 6.24 6.37 6.26 6.34 Mean 6.29 5.88 6.45 6.39 6.26

Table 2. Mean Surface Dissolved Oxygen (mg/L) for Gill Net Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter rea Pre- Post- Pre- Post- Mean SL1 6.27 5.86 6.64 6.61 6.33 SL2 6.56 5.70 6.50 6.47 6.34 SL3 6.74 6.05 6.43 6.53 6.48 Mean 6.52 5.87 T 6.52 T -6.54 6.39

Table 3. Mean Bottom Dissolved Oxygen (mg/L) for Trawl Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter Area Pre- Post- Pre- [ Post- Mean SLI 6.34 6.04 6.54 6.51 6.36 SL2 6.30 5.99 6.51 6.76 6.38 SL3 6.20 6.04 6.50 6.59 6.31 Mean 6.28 6.02 6.52 6.62 6.35

Table 4. Mean Surface Dissolved Oxygen (mg/L) for Trawl Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Area Summer Winter Mean Pre- Post- Pre- Post-SLU 6.53 5.96 6.56 6.50 6.41 SL2 6.47 5.93 6.48 6.73 6.41 SL3 6.34 6.04 6.45 6.51 6.33 Mean 6.45 5.98 6.49 6.58 6.39

Table 5. Mean Bottom pH for Gill Net Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter Area Mean Pre- Post- Pre- Post-SLU 8.00 8.08 8.02 8.25 8.07 SL2 7.99 8.01 8.00 8.10 8.02 SL3 8.05 8.12 7.87 8.11 8.04 Mean 8.01 8.07 7.96 8.15 8.04

Table 6. Mean Surface pH for Gill Net Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter Area Pre- Post- Pre- Post- Mean SL1 7.96 8.05 8.01 8.22 8.05 SL2 7.96 8.00 7.99 8.09 8.00 SL3 8.01 8.10 7.87 8.13 8.02 Mean 7.98 8.05 7.95 8.15 8.02

Table 7. Mean Bottom pH for Trawl Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter Area Pre- Post- Pre- Post- Mean SL1 8.03 8.10 8.00 8.06 8.04 SL2 8.05 8.10 8.01 8.12 8.07 SL3 8.09 8.11 8.00 8.07 8.07 Mean 8.06 8.10 8.00 8.08 8.06

Table 8. Mean Surface pH for Trawl Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Area Summer Winter Mean Pre- Post- Pre- Post-SLU 8.01 8.08 7.99 8.04 8.03 SL2 8.05 8.08 8.01 8.09 8.06 SL3 8.09 8.10 8.00 8.06 8.07 Mean 8.05 8.09 8.00 8.06 8.05

Table 9. Mean Bottom Salinity (PSU) for Gill Net Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Area Summer Winter Mean Pre- Post- Pre- Post-SLI 36.37 35.87 36.19 36.26 36.20 SL2 36.37 36.06 36.12 36.30 36.24 SL3 36.32 35.95 36.09 36.33 36.19 Mean 36.36 35.96 36.14 ] 36.30 36.21

Table 10. Mean Surface Salinity (PSU) for Gill Net Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter AreaPre- Post- Pre- Post- Mean SLU 36.31 35.90 36.21 36.29 36.20 SL2 36.30 35.94 36.17 36.27 36.19 SL3 36.20 35.89 36.11 36.11 36.09 Mean 36.27 35.91 36.16 36.22 36.16

Table 11. Mean Bottom Salinity (PSU) for Trawl Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter rea Pre- Post- Pre- Post- Mean SLU 36.40 36.26 36.41 36.27 36.34 SL2 36.44 36.22 36.45 36.23 36.35 SL3 36.29 36.24 36.51 36.17 36.30 Mean 36.38 36.24 36.45 36.22 36.33

Table 12. Mean Surface Salinity (PSU) for Trawl Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Area Summer Winter Mean Pre- Post- Pre- Post-SLU 36.38 36.25 36.45 36.27 36.34 SL2 36.36 36.17 36.50 36.26 36.33 SL3 36.18 36.15 36.55 36.22 36.26 Mean 36.30 36.19 36.50 36.25 36.31

Table 13. Mean Bottom Specific Conductivity (mS/cm) for Gill Netting Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter Pre- Post- Pre- Post- Mean SLU 54.96 54.23 54.99 54.96 54.81 SL2 54.93 54.48 54.84 54.99 54.83 SL3 54.88 54.39 54.77 55.03 54.78 Mean 54.92 54.37 54.87 54.99 54.81

Table 14. Mean Surface Specific Conductivity (mS/cm) for Gill Net Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Area Pre- J Post- Pre-Winter Post- Mean SLU 54.80 54.24 54.95 54.99 54.75 SL2 54.76 54.21 54.83 54.91 54.69 SL3 54.60 54.16 54.76 54.70 54.56 Mean 54.72 54.21 54.85 T 54.87 54.67

Table 15. Mean Bottom Specific Conductivity (mS/cm) for Trawl Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter Area Pre- S Post- Pre- Post- Mean SLI 54.98 54.69 55.14 54.93 54.94 SL2 55.03 54.70 55.20 54.97 54.98 SL3 54.78 54.78 55.29 54.85 54.90 Mean 54.93 54.72 [ 55.21 54.92 54.94

Table 16. Mean Surface Specific Conductivity (mS/cm) for Trawl Sampling by Area and Season for Pre- and Post- Uprate Sampling Events, St. Lucie Plant EPU.

Area Summer Winter Mean Pre- Post- Pre- Post-SLI 54.87 54.67 55.19 54.93 54.91 SL2 54.87 54.53 55.23 54.99 54.90 SL3 54.54 54.51 55.30 54.86 54.77 Mean 54.76 54.57 55.24 54.93 54.86

Table 17. Mean Bottom Temperature ('C) for Gill Net Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter Area Pre- Post- Pre- Post- Mean SLU 24.60 25.34 21.50 22.82 23.71 SL2 25.08 25.46 22.00 22.86 24.03 SL3 24.88 24.96 22.30 23.27 24.01 Mean 24.85 25.26 21.93 22.98 23.91

Tablel8. Mean Surface Temperature (IC) for Gill Net Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter Area Pre- Post- Pre- Post- Mean SLI 25.53 26.05 21.84 22.98 24.31 SL2 26.05 27.14 22.70 23.62 25.04 SL3 26.28 27.14 22.74 23.84 25.18 Mean 25.95 26.78 22.43 23.48 24.84

Table 19. Mean Bottom Temperature (IC) for Trawl Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Area Summer IMeanPre- Winter Post-Pre- Post-SL1 24.90 25.72 22.82 23.09 24.24 SL2 24.94 25.22 23.04 21.94 23.95 SL3 25.59 24.96 22.90 22.49 24.22 Mean 25.15 25.30 J 22.92 [ 22.51 24.14

Table 20. Mean Surface Temperature (IC) for Trawl Sampling by Area and Season for Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Summer Winter rea Pre- Post- Pre- Post- Mean SLI 25.66 26.36 22.91 23.12 24.67 SL2 25.72 26.41 23.21 22.21 24.58 SL3 26.52 26.55 23.27 23.10 25.09 Mean 25.96 26.44 23.13 22.81 24.78

Table 21. Common and Scientific Names of Fish, Invertebrates, and Reptiles Identified During 14 Sampling Events (August 2011 - January 2014), St. Lucie Plant EPU. Taxa in Bold are Representative Important Species (RIS).

Taxa lCommon Name Cephalopoda squid Gastropoda Gastropod Idiosepiidae pygmy cuttlefish Loliginidae squid Loligo pealeli Iongfin inshore squid Myopsida myopsid squids Opisthobranchia seahare Crustacean, Acetes americanus aviu shrimp Albunea sp.* mole crabs Albuneidae* mole crabs Arenaeus cribrarius* speckled swimming crab Automate sp. snapping shrimp Calappaflammea flame box crab Calappoidea box crabs Callinectes ornatuis shellig Callinectes sapidus* blue crab Callinectessimilis lesser blue crab Callinectes sp. swimming crabs Caridea caridean shrimp Chlamydopleon dissimile opossum shrimp Decapoda decapod crustaceans Emerita sp.* mole crab Emerita talpoida* Atlantic sand crab Farfantepenaeusaztecus* brown shrimp Farfantepenaeusduorarum

• pink shrimp Farfantepenaeus sp.* penaeid shrimp Hepatus epheliticus calico crab Hexapanopeus angustifrons smooth mud crab Hippidae sand crabs

Taxa Common Name Hippoidea mole crab Latreutesfiicorum slender sargassum shrimp Latreutesparvulus sargassum shrimp Latreutes sp. sargassum shrimp Leander cf. tenuicornis brown grass shrimp Lepidopa sp. mole crab Lepidopa websteri Webster's mole crab Leptochela carinata carinate glass shrimp Leptochela serratorbita combclaw shrimp Leucosioidea purse crabs Libinia dubia longnose spider crab Lysmata rathbunae pathbun cleaner shrimp Lysmata sp. cleaner shrimp Majidae spider crab Majoidea spider crabs Menippe mercenaria* Florida stone crab Menippe sp.* stone crab Ogyrides hayi sand longeye shrimp Ovalipes ocellatus lady crab Ovalipes sp. lady crab Paguridae right-handed hermit crabs Paguroidea hermit crab Palaemonidae grass shrimps Pandalidae pandalid shrimp Panulirusargus* Caribbean spiny lobster Penaeidae* penaeid shrimp Periclimeneslongicaudatus longtail grass shrimp Periclimenes sp. anemone shrimp Persephona mediterranea mottled purse crab Petrochirusdiogenes giant hermit crab Phycomenes siankaanensis iridescent shrimp Piluhnus sp. hairy crab Pitho sp. urn crabs Podochela sp. spider crab

Taxa Common Name Portunidae swimming crabs Portunusanceps delicate swimming crab Portunus gibbesii iridescent swimming crab Portunussayi sargassum swimming crab Portunus sp. portunid crab Portunus spinimanus blotched swimming crab Processahemphilli night shrimp Rinapenaeus constrictus* roughneck shrimp Rimapenaeus sp. roughneck shrimp Scyllaridae* slipper lobsters Scyllarus americanus* American slipper lobster Sicyonia brevirostris* brown rock shrimp Sicyonia sp.* rock shrimp Sicyonia typica* kinglet rock shrimp Sicyoniidae* rock shrimps Squillidae mantis shrimp Stomatopoda mantis shrimp Synalpheus sp. snapping shrimp Trachypenaeopsisrichtersii Richter sand shrimp Unidentified shrimp unidentified shrimp Upogebiidae mud shrimps Xanthidae mud crabs Xiphopenaeus kroyeri seabob Echinodermata Arbaciapunctulata purple-spined sea urchin Arbacioida sea urchin Asteroidea sea stars Astropecten sp. sea star Clypeasteroida sand dollars Echinodermata Echinoderm Echinoidea sea urchins and sand dollars Encope sp. sand dollar Lytechinus variegatus green sea urchin Mellita quinquiesperforata sand dollar

Taxa Common Name Mellitidae sand dollars Ophiuroidea brittle stars Temnopleuroida sea urchins

• fish, Acanthostracionquadricornis scrawled cowfish Acanthurus sp. surgeonfish Achiridae American/scrawled soles Achirus lineatus lined sole Acropomatidae temperate ocean-basses Aetobatus narinari spotted eagle ray Albula sp. bonefishes Albula vulpes bonefish Alosa sp. herring Aluterus mnonoceros Unicorn Filefish Aluterus schoepfii orange filefish Anchoa cubana Cuban anchovy Anchoa hepsetus striped anchovy Anchoa lyolepis dusky anchovy Anchoa mitchilli bay anchovy Anchoa sp. common anchovy Ancylopsetta omninata oscellated flounder Anguilloidei eels Anisotremus surinamensis black margate Anisotremus virginicus porkfish Apogon binotatus barred cardinalfish Apogonidae cardinalfish Archosargusprobatocephalus sheepshead Archosargus rhomboidalis sea bream Ariopsisfelis hardhead catfish Astrapogonpuncticu/atus blackfin cardinalfish Atheriniformes silversides Atherinopsidae New World silversides Bagre marinus gafftopsail catfish Bairdiellachrysoura silver perch

Taxa Common Name Balistidae triggerfishes Bathygobius soporator frillfin goby Blenniidae combtooth blennies Bothidae lefteyed flounders Bothus robinsi twospot flounder Bramidae pomfrets Bregmacerotidae codlets Brevoortiasmithi yellowfin menhaden Brevoortia sp. menhadens Brevoortiatyrannus Atlantic menhaden Calamus arctifrons grass porgy Carangidae jacks Carangidae/ Labridae/ Sciaenidae egg complex egg complex: jacks/ wrasses/ drum:

Carangoidesbartholonaei yellow jack Caranx crysos blue runner Caranx hippos crevalle jack Caranx latus horse-eye jack Caranx sp. jack Carcharhinusacronotus blacknose shark Carcharhinusbrevipinna spinner shark Carcharhinuslimbatus blacktip shark Centropomus undecimalis common snook Centropristisphiladelphica rock sea bass Centropristisstriata black sea bass Cerdalefloridana Pugjaw wormfish Chaetodipterusfaber Atlantic spadefish Chasmodes saburrae Florida blenny Chloroscombrus chnvsurus Atlantic bumper Citharichthysarctifrons Gulf Stream flounder Citharichthysmacrops spotted whiff Citharichthys sp. whiff Citharichthysspilopterus bay whiff Clupeidae herrings and sardines Clupeiformes herring-like fishes

Taxa Common Name Congridae conger eels Corvula sanctaeluciae striped croaker Cotyphaena hippurus dolphin, mahi mahi, dorado Ctenogobius boleosomna darter goby Ctenogobius sp. goby Cyclothone sp. bristlefish Cynoscion nebulosus spotted sea trout Cynoscion nothus silver seatrout Cynoscion regalis gray trout Cynoscion sp. seatrouts Dactyloscopidae sand stargazers Dactyloscopus crossotus bigeye stargazer Decapterusmacarellus mackerel scad Decapteruspunctatus round scad Diapterusauratus Irish pompano Diodon sp. porcupinefish Diodontidae burrfishes Diogenichthys atlanticus Atlantic lanternfish Diplectrumformosumn sand perch Diplectrum sp. sand perch Diplodus holbrookii spottail pinfish Diplospinusmultistriatus striped escolar Dormnitator inaculatus fat sleeper Dorosomapetenense threadfin shad Echeneis naucrates sharksucker Eleotridae sleepers Eleotrispisonis Spinycheek sleeper Elops saurus ladyfish Engraulidae anchovies Engrauliseurystole silver anchovy Epinephelus sp. groupers Etrumeus teres round herring Eucinostomus argenteus spotfin mojarra Eucinostomus gula silver jenny

Taxa Common Name Eucinostomus harengulus tidewater mojarra Eucinostomusjonesii slender mojarra Eucinostoinus sp. mojarra Euthynnus alletteratus little tunny Gempylidae snake mackerels Gerreidae mojarra Gobiesox strumosus skilletfish Gobiidae gobies Gobionellus oceanicus highfin goby Gobiosoma bose naked goby Gobiosomaparri naked gobie Gobiosoma robustum code goby Gobiosoma sp. goby Gonostomatidae lightfishes Gymnachirus melas naked sole Haemulidae grunts Haemulon aurolineatum tomtate Haemuilon macrostomum spanish grunt Haemulon sp. grunt Halichoerescaudalis painted wrasse Harengulahumeralis redear sardine, redear herring Harengulajaguana scaled sardine Hemiramphus brasiliensis ballyhoo Hippocampus erectus spotted seahorse Hygophum reinhardtii lanternfishes Hyporhamphus unifasciatus Atlantic silverstripe halfbeak Labridae wrasses Labrisomidae labrisomid blennies Labrisomusnuchipinnis hairy blenny Lagodon rhomboides pinfish Larimusfasciatus banded drum Leiostomus xanthurus spot Lupinoblennius nicholsi highfin blenny Lutjanidae snappers

Taxa Common Name Lutjanus analis mutton snapper Lutjanus griseus gray snapper Lutjanus sp. snapper Lutjanus synagris lane. snapper Melarnphaidae bigscale fishes Melanocetidae deepsea anglerfishes Membras martinica rough silverside Menticirrhusamericanus southern kingfish Menticirrhus littoralis Gulf kingfish Menticirrhus saxatilis northern kingfish Menticirrhus sp. kingfishes/ Weakfishes Merlucciidae hakes Microdesmidae wormfishes Microdesmus sp. wormfishes Microgobiusgulosus clown goby Microgobius thalassinus green goby Micropogoniasfitrnieri whitemouth croaker Micropogonias undulatus Atlantic croaker Monacanthidae filefishes Monacanthusciliatus fringed filefish Mugil cephalus striped mullet Mugil curema silver mullet Mullidae goatfishes Mustelus canis smooth dogfish Myctophidae lantemfishes Mliiobatisgoodei southern eagle ray Narcine bancroftii lesser electric ray Nes longus orangespotted goby Ogcocephalus sp. batfish Oligoplites saurus leatherjacket Ophidiidae cusk eels Ophidiiformes pearlfishes and cusk-eels Ophidion grayi blotched cusk-eel Ophidion holbrookii bank cusk eel

Taxa Common Name Ophidion sp. cusk-eel Opisthonema oglinum Atlantic thread herring Opistognathidae jawfishes Opistognathusrobinsi spotfin jawfish Opsanus beta Gulf toadfish Orthopristischrysoptera pigfish Ostraciidae boxfishes/truckfishes Parablenniusmarmoreus seaweed blenny Paralepididae barracudinas Paralichthyidae sand flounders Paralichthysalbigutta Gulf flounder Pareques acuminatus high-hat Pareques sp. drum Peprilusburti gulf butterfish Peprilusparu harvestfish Perciformes perch-like fishes Percophidae duckbills Phosichthyidae lightfishes Pleuronectiformes flounders Polydactylus oligodon littlescale threadfin Polydactylus virginicus barbu Pomacanthidae angelfishes Pomacentridae damselfishes Pomatomus saltatrix bluefish Prionotusrubio blackwing searobin Prionotusscitulus leopard searobin Prionotus sp. North American searobins Prionotustribulus bighead searobin Pseudocaranxdentex white trevally Rachycentron canadum cobia Raja eglanteria clearnose skate Rhinobatos lentiginosus Atlantic guitarfish Rhizoprionodon terraenovae Atlantic sharpnose shark Sardinella aurita Spanish sardine

Taxa Common Name Saurida brasiliensis largescale lizardfish Scaridae parrotfishes Scarus sp. parrotfish Sciaenidae drums and croakers Sciaenops ocellatus red drum Scomberomorus maculatus Atlantic spanish mackerel Scombridae mackerels Scorpaena brasiliensis barbfish Scorpaena grandicornis plumed scorpionfish Scorpaenaplumieri spotted scorpionfish Scorpaenidae scorpionfishes Scorpaeniformes scorpionfishes Selar crumenophthalinus bigeyed scads Selene setapinnis Atlantic moonfish Selene vomer lookdown Serranidae sea basses and groupers Sparidae porgies Sparisoma sp. parrotfishes Sphoeroides sp. puffer Sphyraenidae barracuda Sphyrna tiburo bonnethead shark Stellifer lanceolatus star drum Stephanobetyciformes stephanoberyciforms Stephanolepis hispida planehead filefish Stomiiformes stomiiforms Symphurus diomedeanus spottedfin tonguefish Symphurus sp. straightmouth tonguefishes Syngnathidae pipefishes Syngnathusfiiscus northern pipefish Syngnathus louisianae chain pipefish Svngnathus sp. pipefishes Synodontidae lizard fish Synodusfoetens inshore lizardfish Tetraodontidae puffers

Taxa Common Name Tetraodontiformes puffers Trachinocephalusmyops bluntnose lizardfish Trachinotuscarolinus Florida pompano Trachinotusfalcatus permit Trachinotusgoodei palometa Trichiurus lepturus cutlassfish Triglidae searobins Trinectes maculatus hogchoker Umbrina coroides sand drum Unidentified eggs unidentified eggs Unidentified fish unidentified fish Unidentified fragment unidentified fragment Uranoscopidae stargazers RRepfile Chelonia n'das green sea turtle

• Commercially and recreationally important (CR1) decapod crustaceans

Table 22. Number of Molluscs, Crustaceans, Echinoderms, and Fish Captured by Trawl for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SLI SL2 SL3 Trawl Taxa Summer Winter Summer =Winter Summer Winter Total Pre Post Pre Post Pre Post Pre -Post Pre Post Pre Post Mollusca _____ ... ... . . ..... __.... ____."

Gastropoda 27 27 Opisthobranchia 18 1 19 Loliginidae 1 21 Idiosepiidae 1 1 Myopsida 1 1 Total [1 11 18 127121 2_ ___ __0 5_

Crustacean Acetes americanus 92 16 1304 3 1 15 2 1433 Portunusspinimanus 14 5 51 94 3 3 170 Rimapenaeus sp. 22 3 5 13 12 24 10 12 38 7 12 2 160 Paguroidea 101 6 3 7 1 2 1 12 15 4 1 153 Portunusgibbesii 23 7 11 17 1 2 23 23 2 12 4 125 Arenaeus cribrarius* 15 7 8 1 7 7 8 53 Leander cf. tenuicornis 50 50 Farfantepenaeusduorarum* 10 4 2 1 1 13 6 37 Latreutesfucorum 36 36 Portunits sp. 9 6 4 2 6 2 4 1 34 Rimapenaeus constrictus* 1 14 7 7 3 32 Hepatus epheliticus 3 2 2 1 1 2 13 4 1 29 Farfantepenaeussp.* 6 17 2 25

S__ SL2 S-Trawl Taxa Summer Winter Summer Winter Summer Winter Total Pre LPost Pre Post Prel Post Pre Post Pre Post Pre Post Penaeidae* 8 1 3 4 1 2 3 3 25 Paguridae 22 22 Calappoidea 14 1 3 18 Portunidae 15 2 17 Portunussavi 13 1 14 Portunus anceps 1 8 1 1 1 12 Sicyonia brevirostris* 6 2 2 10 Callinectes sp. 3 1 4 8 Majoidea 5 1 1 1 8 Libinia dubia 4 3 7 Podochela sp. 3 4 7 Decapoda 5 1 6 Persephonamediterranea 2 1 1 2 6 Processahemnphilli 5 1 6 Falfantepenaeusaztecus* 2 3 5 Calappaflanimea 1 1 2 4 Ovalipes ocellattus 2 1 1 4 Periclimenes longicaudatus 3 1 4 Caridea 3 3 Automate sp. 1 2 Callinectes ornatus 2 2 Callinectessapidus* 2 2 Chlamvdopleon dissimile 1 1 2

SL1 SL2 SL3 Trawl Taxa Summer Winter Summer Winter Summer Winter Total LPre I Post I Pre I Post Pre IPost Pre Post Pre Post Pre Post Latreutesparvulus 2 2 Panulirusargus* 2 2 Periclimenes sp. 1 1 2 Stomatopoda 1 1 2 Hexapanopeusangostifirons 1 1 Latreutes sp. 1 1 Leptochela carinata 1 1 Leptochela serratorbita 1 1 Leucosioidea 1 1 Lysmata rathbunae 1 1 Lysmata sp. 1 1 Majidae 1 1 Ogyrides hayi 1 1 Ovalipes sp. 1 1 Palaemonidae 1 1 Pandalidae 1 1 Petrochirusdiogenes 1 1 Phycomenes siankaanensis 1 1 Pilumnus sp. 1 1 Pitho sp. 1 1 Sicyonia tipica* 1 1 Sicyoniidae* 1 1 Synalpheus sp. 1 1

SL1 SL2 SL3 Trawl Taxa Summer Winter Summer Winter Summer Winter Total I Pre Post Pre [Post I Pre I Post Pre ] Post Pre I Post Pre [Post Trachypenaeopsisrichtersii 1 1 Upogebiidae 1 1 Xanthidae 1 1 Total .. 359 162 19 70 11443 81 20 s 52 240 55 __42 __18 _ 2561 Echinodermata.

Clypeasteroida 124 19 153 6 23 15 76 3 10 3 432 Temnopleuroida 19 11 13 11 1 32 11 98 Arbaciapunctzdata 16 3 19 Asteroidea 17 2 19 Ophiuroidea 1 5 6 12 Echinoidea 1 1 2 3 7 Arbacioida 1 1 Total 11-44136 170 6j 37 _1.51891 4 49] , _35 3~ 588 Fish ""_ __ __._",

Engraulidae** 181 103 4 288 Anchoa cubana** 229 48 10 287 Umbrina coroides** 46 149 4 21 3 4 4 18 15 8 272 Stellifer lanceolatus 31 72 30 133 Chloroscombruschrvsurus 23 11 1 1 31 1 34 3 1 106 Haernulonaurolineatum 53 1 20 74 Harengulajaguana** 62 6 68 Eucinostomus gula 34 3 10 47 Citharichthysmacrops 7 10 1 1 21 2 42

SL1 SL2 SL3 Trawl Taxa Summer Winter Summer Winter Summer Winter Total Pre IPost Pre! Post --Pre IPost- 1-- Postot Pr Pre Post]

Prionotusscitulus** 2 1 2 9 1 3 14 8 40 Anchoa sp.** 4 4 12 1 21 Ophidion sp. 5 12 1 18 Menticirrhuslittoralis** 7 1 1 2 2 1 1 15 Corvula sanctaeluciae 2 2 6 4 14 Lutianus synagris 5 1 1 7 14 Micropogoniasundulatus** 1 1 4 8 14 Bothus robinsi 10 2 1 13 Selene setapinnis 2 1 1 4 5 13 Ophidion holbrookii 1 1 2 8 12 Ophidion grayi 10 10 Cynoscion nothus** 1 4 4 9 Eucinostomusjonesii 9 9 Larimusfasciatus 8 1 9 Leiostomnus xanthurus** 6 3 9 Synodusfoetens 4 1 4 9 Sardinellaaurita** 1 7 8 Selene vomer 4 2 1 1 8 Stephanolepishispida 4 3 1 8 Lagodon rhomboides 2 2 1 5 Caranxcrysos 2 1 1 4 Centropristisstriata 2 2 4 Opisthonema oglinum ** 1 3 4

St 1 SL2 SL3 Trawl Taxa Summer Winter Summer Winter Summer Winter Total

_Pre IPost Pre Post Pre [ Post Pre [Post Pre [Post-Post Pre Eucinostomus harengulus 1 2 3 Labrisomus nuchipinnis 3 3 Prionotusrubio 12 3 Sciaenidae 1 2 3 Scorpaenabrasiliensis 1 2 3 Achirus lineatus 1 1 2 Anchoa hepsetus** 1 1 2 Anchoa mitchilli** 1 1 2 Caranx hippos 2 2 Cynoscion regalis 1 1 2 Decapteruspunctatus 2 2 Diplectrumnformosum 1 1 2 Monacanthus ciliatus 2 2 Peprilusparu 1 1 2 Symphurus diomedeanus 1 1 2 Trichiurus lepturus 1 1 2 Anchoa lyolepis** 1 1 Anisotremus surinamensis 1 1 Anisotremus virginicus 1 1 Apogon binotatus 1 1 Ariopsisfelis 1 1 Caranx latus 1 1 Centropristisphiladelphica 1 1

SLi SL2 SL3 Trawl Taxa Summer Winter Summer__ Winter Summer Winter Total

_ Pe

_Pre IostPPost Pre Post Pre [Post Pre IPost I Pre IPost Citharichthysspilopterus 1 1 Cynoscion nebulosus 1 1 Dactyloscopus crossotus 1 1 Diapterusauratus 1 1 Elops saurus 1 1 Gymnachirus melas 1 1 Haemulon macrostomuin 1 1 Haemulon sp. 1 1 Halichoeres caudalis 1 1 Hippocampus erectus 1 1 Labridae 1 1 Narcine bancrofiii 1 1 Oligoplites saurus 1 1 Opistognathusrobinsi 1 1 Orthopristischrysoptera** 1 1 Polvdactylus virginicus 1 1 Prionotus sp. 1 1 Raja eglanteria I 1 Scorpaenagrandicornis 1 1 Serranidae 1 1 Syngnathusfuscus 1 1 Trachinocephalusmyops 1 1 Trachinotus carolinus** I 1

SL1 SL2 SL3 Trawl Taxa Summer Winter Summer Winter Summer Winter Total

[ Pre ]Post Pre [Post Pre [Post Pre [Post Pre [Post- re I Post Trinectes maculatus ___I___I___ ___I___I___ ___I___I J__ _______ 1 Total 719 187 32 32 337 11 17 15 241 32 15 17 1655 Trawl Total 1223 403 221 108 1844 109 126 71 532 87 92 38 4854

• Commercially or Recreationally Important Decapod Crustaceans

• • Representative Important Species

Table 23. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Number of Taxa, Catch Per Unit Effort (CPUE), and Biomass Among Areas for Fish, RIS Fish, and CRI Invertebrates Captured by Trawl During All Events, Seasons, Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Fish Taxa All Events 126 0.7074 0.7021

+ 3.12 +2.99 +/-3.68 2.96 2.83 3.08 Pre-uprate 2 72 0.0332 0.9835

+ 3.65 +_3.61 +/-_4.36 1.39 0.78 0.78 Post-uprate 2 54 4.1186 0.1275

• 1.97 +/- 1.17 +/- 1.93 2.79 2.63 2.92 Summer 2 72 0.2152 0.8980

• 3.37 + 3.60 +/- 4.43 Winter 1.61 1.06 1.00 2 54 0.5829 0.7472

• 2.68 +/- 1.59 + 1.97 Fish CPUE 33.37 13.50 10.58 All Events 2 126 0.5935 0.7432

+/-_90.03 +_34.56 +/- 20.48 47.22 22.17 14.64 Pre-uprate 2 72 0.1565 0.9247

+/- 111.88 +/-44.03 +/- 23.40 14.91 1.93 5.17 Post-uprate 2 54 4.0852 0.1297

+/- 44.49 +_3.09 +/- 14.72 55.25 22.13 16.77 Summer 2 72 0.3075 0.8575

+/- 115.03 +/-44.04 +/- 25.13 4.20 1L98 2.33 Winter 2 54 0.5710 0.7517

+/- 8.28 +/-_3.20 +/- 5.57 1 1

Period 8.78 9.38S All Events 18.82 8.78 9.38 2 126 0.5003 0.7787

+46.31 +/- 19.68 +/- 19.22 22.09 14.15 13.82 Pre-uprate 2 72 0.0978 0.9523

+ 49.17 +/- 24.80 +/- 23.56 14.45 1.62 3.47 Post-uprate 2 54 2.4193 0.2983

+/- 43.19 +/-2.49 +8.69 29.84 14.11 14.80 Summer 2 72 0.3159 0.8539

+/-_58.96 +_24.81 +/-_23.86 4.13 1.67 2.17 Winter 2 54 0.2930 0.8637

+ 8.31 +/-_2.63 +/-_4.92 RIS Fish Taxa 0.88 0.74 0.67 All Events 0.807 .72 126 0.4308 0.8062 A e1.35 +/-+1.27 +1.05_ _ _ _______' _

0.96 1.04 0.88 Pre-uprate 2 72 0.5585 0.7563

+/- 1.49 +/- 1.52 +/- 1.03 0.78 0.33 0.39 Post-uprate 2 54 5.3856 0.0677

+/-1.17 +/-0.69 +/-1.04 0.96 0.96 0.83 Summer 2 72 0.0158 0.9921

+/- 1.43 +/- 1.49 +/- 1.09 0.78 0.44 0.44 Winter 2 54 1.4274 0.4898

+/- 1.26 +/- 0.86 +/- 0.98

RIS CPUE All Events 24.18 6.64 3.94 2 126 0.6950 0.7064

+/- 76.85 -24.06 +/-10.12 O_.

32.97 10.71 3.68 Pre-uprate 2 72 0.4968 0.7800

• 95.92 +/-31.41 +5.49 12.46 1.21 4.28 Post-uprate 1.21 4.28 246 54 1.8509 0.0641

_______ +/-39.27

• +/-2.66 +/-14.35 _____ ____ ____

40.37 10.72 5.88 Summer 2 72 0.1195 0.9420

_- 99.35 +/-31.42 +/-_12.75 2.59 1.20 1.35 Winter 2 54 1.6653 0.4349 1-5.06 +/- 2.55 +/- 3.81 RIS Fish CPUE Excluding Engraulidae All Events 9.63 1.93 2.74 2 2 .26 088

_- 29.31 +/-3.59 +/-6.20 7.84 2.69 2.85 Pre-uprate 2 72 0.3293 0.8482 4-21.43 4.29 4.59 12.00 0.90 2.59 Post-uprate 2 54 3.6086 0.1646

• 37.95 +/- 2.04 +/- 8.00 14.96 2.70 3.91 Summer 2 72 0.0324 0.9839

• - 37.99 4.34 +/- 7.60 2.52 0.89 1.18 Winter 2 54 0.9634 0.6177 4- 5.09 +/-- 1.89 +/- 3.15 _ 1_

RIS Fish Biomass 128.95 59.98 75.36 All Events 2 126 0.6534 0.7213

+ 391.26 - 181.04 +/-246.04 200.90 102.78 130.23 Pre-uprate 2 72 0.6494 0.7227

+ 502.31 +/-232.24 +/-317.09 33.01 Post-uprate +/- 103.89 2.92 2.21 2 54 6.4765 0.0392

+ 6.63 +/- 7.09 168.96 59.78 113.05 Summer 2 72 0.2810 0.8689

+ 484.79 +/- 141.03 +319.40 75.60 60.25 25.11 Winter 2 54 0.7656 0.6819 38

• 214.40 228.34 +/- 57.40 RIS Fish Biomass Excluding Engraulidae 5.581 59.772 5.03 All Events 2 126 0.3174 0.8533

• 237.95 +/- 164.30 +/- 246.08 Pre-uprate 128 86913.6272 0.3238 0.8505

+/- 295.74 +/- 211.91 +/- 317.16 Post-uprate 3.8 24210?54 3.9326 0.1400

+/- 111.38 4-5.85 +/-6.88 Summer 122 43911.6272 0.0947 0.9538

+/- 258.14 +/- 97.14 +/-319.47 Witr 75.58 59.77 25.03254033 1 +/- 214.41 +/- 228.45 +/- 57.42 E

CRI Invertebrate Taxa 0.74 0.69 0.79 All Events 2 126 0.1430 0.9310

+/-1.13 +/-0.84 -1.14 0.88 0.75 0.92 Pre-uprate 2 72 0.0287 0.9858

+/- 1.19 +/- 0.79 +/- 1.28 0.56 0.61 0.61 Post-uprate 2 54 0.1414 0.9318

_ 1.04 +/-_0.92 +/- 0.92 0.75 0.67 1.17 Summer 2 72 2.1392 0.3431 1.11

+/- +/- 0.76 +/- 1.31 7:3] 0.2912 0.72 0.72 0.28 Winter 2 54 2.4673 0.2912

-_-1.18 +/-_0.96 +/-_0.57 ___

CRI Invertebrate CPUE All Events 126 0.4620 0.4937

+/- 10.40 +/-_6.73 +/-_6.84 5.25 3.97 4.95 Pre-uprate 2 72 0.4757 0.7883

+ 10.11 +/- 6.57 +/- 7.98 4.11 3.42 2.65 Post-uprate 2 54 0.0746 0.9634

+/- 11.03 +/-7.12 +/-4.83 5.28 4.85 5.77 Summer 2 72 1.8307 0.4004

+/- 10.16 +/-8.30 +/-8.13 Winter 4.06 2.25 1.55 2 54 1.5716 0.4558

+/- 10.96 +/- 3.46 +/-3.54

Table 24. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Number of Taxa, Catch Per Unit Effort (CPUE), and Biomass Among Pre- and Post-Uprate Events Within Each Area for Fish, RIS Fish, and Invertebrates Captured by Trawl, St. Lucie Plant EPU.

Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Fish Taxa 2.96 1.39 SLI 1 42 0.5087 0.4757

+3.65 +1.97 2.83 SL2 +/- 3.61 0.78 1 42 4.6488 0.0311

+ 1.17 3.08 SL3 +/- 4.36 0.78 1 42 6.5632 0.0104

+_1.93 Fish CPUE

• . Are Pr-prt" Potur l47.22 14.91 SL 1 1 42 0.0438 0.8343

+/- 111.88 +44.49 22.17 SL2 +/- 44.03 1.93 1 42 4.0035 0.0454

+/- 3.09 14.64 SL3 +/- 23.40 5.17 1 42 5.7158 0.0168

+/- 14.72 1 1 Fish CPUE Excluding Engraulidae 22.09 14.45 SLI 1 42 0.2026 0.6526

+/- 49.17 +43.19 14.15 SL2 +/- 24.80 1.62 1 42 4.4420 0.0351

+_2.49 13.82 SL3 +/- 23.56 3.47 1 42 5.0098 0.0252

+/- 8.69 _ _ _

RIS Fish Taxa SL1 .607 2026 0.6417

• 1.49 +/- 1.17 1.04 0.33 SL2 42 3.2987 0.0693 S1.52 +/-0.69 0.88 SL3 +/- 1.03 0.39 1 42 5.5647 0.0183

+1.04 1 RIS CPUE Aa r . 3. 1)H 32.97 12.46 SL1 1 42 0.3740 0.5408

+/- 95.92 +/- 39.27 10.71 1.21 SL2 1 42 2.9473 0.0860

+/- 31.41 +2.66 3.68 4.28 SL3 3.4

+5.49 4.35

+14.35 1 42 0.8206 0.4119 RIS Fish CPUE Excluding Engraulidae SL1 7.84 12.00 1 42 0.1604 0.6888

+21.43 +/-37.95__________

2.69 0.90 SL2 1 42 3.1505 0.0759

+/- 4.29 +/- 2.04 2.85 25 SU +/-4.59 +8.Q00 1 42 1.6648 0.0960 RIS Fish Biomass 200.90 33.01 SLI 1 42 0.0124 0.9115

+/- 502.31 +/-103.89 102.78 SL2 +/- 232.24 2.92 1 42 4.0666 0.0437

+/- 6.63 130.23 SL3 +/- 317.09 2.21 1 42 8.3487 0.0039

+/- 7.09

RIS Fish Biomass Excluding Engraulidae 132.84 34.78 SLI 1 42 0.0818 0.7748

+ 295.74 -111.38 86.94 SL2 +/- 211.91 2.43 1 42 4.1871 0.0407

+/- 5.85 130.06 SL3 317.16 2.10 1 42 6.3540 0.0117

+_6.88 CRI Invertebrate Taxa SLI 1 42 0.9035 0.3418 SL2 1 42 0.7251 0.3945

+/- 0.79 +/- 0.92 0.92 0.61 SL3 1 42 0.4260 0.5140

+-1.28 +/- 0.92 CRI Invertebrate CPUE SLI 5.25 4.11 1 42 0.3734 0.5412

+10.11 -11.03 _

3.97 3.42 SL2 1 42 1.0891 0.2967

+/--6.57 +/--7.12 4.95 2.65 SL3 1 42 0.7913 0.3737

+/--7.98 +/- 4.83

Table 25. CPUE (Number Collected per Kilometer Trawled) for All Taxa Captured by Trawl for All Events, by Area, Season, and Pre- and Post-Uprate, St. Lucie Plant EPU.

SL1 SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total' Pre Post Pre [ Post Pre J Post Pre Post Pre IPost Pre Post Mollusc . . . .. . ". . _"_

Gastropoda 2.68 0.27 Opisthobranchia 2.45 0.14 0.19 Loliginidae 0.14 0.09 0.02 Idiosepiidae 0.09 0.01 Myopsida 0.10 0.01 Crustaceans Acetes americanus 9.52 2.17 129.36 0.42 0.15 1.42 0.32 14.20 Portunus spinimanus 1.45 0.68 5.06 8.93 0.46 0.33 1.68 Rimapenaeus sp. 2.28 0.41 0.49 1.87 1.19 3.35 0.97 1.82 3.61 1.06 1.30 0.32 1.59 Paguroidea 10.46 0.82 0.30 1.01 0.14 0.19 0.15 1.14 2.28 0.43 0.16 1.52 Portunus gibbesii 2.38 0.95 1.58 1.69 0.14 0.19 3.48 2.18 0.30 1.30 0.64 1.24 Arenaeus cribrarius* 1.55 1.01 0.79 0.14 1.06 0.66 1.21 0.53 Leander cf. tenuicornis 6.80 0.50 Farfantepenaeusduorarum* 1.04 0.54 0.20 0.10 0.10 1.23 0.91 0.37 Latreutesfucorum 4.89 0.36 Portunus sp. 0.93 0.82 0.57 0.20 0.84 0.30 0.38 0.15 0.34 Rimapenaeus constrictus* 0.14 2.01 7.00 7.00 3.00 0.32 Hepatus epheliticus 0.31 0.20 0.20 0.14 0.10 0.30 1.23 0.43 0.16 0.29 Faifantepenaeus sp.* 0.62 1.69 0.19 0.25 Penaeidae* 0.83 0.14 0.30 0.56 0.15 0.19 0.46 0.33 0.25

SL1 SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total' Pre I Post Pre I Post _ Pre _Post Pre I Post Pre I Post Pre I Post Paguridae 33.07 0.22 Calappoidea 1.45 0.10 0.28 0.18 Portunidae 1.55 0.20 0.17 Portunussayi 1.77 0.09 0.14 Portunus anceps 0.14 0.79 0.10 0.15 0.11 0.12 Sicyonia brevirostris* 0.60 0.19 0.22 0.10 Callinectes sp. 0.31 0.10 0.57 0.08 Majoidea 0.52 0.14 0.15 0.11 0.08 Libinia dubia 0.57 0.42 0.07 Podochela sp. 0.41 0.56 0.07 Decapoda 0.52 0.09 0.06 Persephonamediterranea 0.21 0.14 0.09 0.32 0.06 Processahemphilli 0.50 0.14 0.06 Farfantepenaeusaztecus* 0.29 0.29 0.05 Calappaflammea 0.14 0.15 0.32 0.04 Ovalipes ocellatus 0.21 0.10 0.09 0.04 Periclimeneslongicaudatus 0.31 0.10 0.04 Caridea 0.41 0.03 Automate sp. 0.10 0.10 0.02 Callinectes ornatus 0.20 0.02 Callinectes sapidus* 0.28 0.02 Chlamydopleon dissimile 0.14 0.15 0.02

SLU SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total1 Pre Post Pre I Post Pre Post Pre Post Pre Post Pre _Post Latreutesparvulus 0.28 0.02 Panulirusargus* 0.27 0.02 Periclinienes sp. 0.14 0.15 0.02 Stomatopoda 0.10 0.10 0.02 Hexapanopeusangustifirons 0.10 0.01 Latreutes sp. 0.10 0.01 Leptochela carinata 0.16 0.01 Leptochela serratorbita 0.10 0.01 Leucosioidea 0.10 0.01 Lysmata rathbunae 0.10 0.01 Lysinata sp. 0.10 0.01 Majidae 0.14 0.01 Ogyrides hayi 0.10 0.01 Ovalipes sp. 0.14 0.01 Palaemonidae 0.14 0.01 Pandalidae 0.14 0.01 Petrochirusdiogenes 0.15 0.01 Phycomenes siankaanensis 0.14 0.01 Pilumnus sp. 0.14 0.01 Pitho sp. 0.14 0.01 Sicyonia typica* 0.10 0.01 Sicyoniidae* 1 0.09 0.01

SL1 SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total1 Pre J Post Pre Post Pre Post Pre [Post Pre [ Post Pre IPost Synalpheus sp. 0.14 0.01 Trachypenaeopsisrichtersii 0.14 0.01 Upogebiidae 0.10 0.01 Xanthidae 0.14 0.01 Ecine".de.rms .. .

Clypeasteroida 12.84 2.58 15.07 1.15 2.28 2.09 7.37 0.61 1.08 0.48 4.31.

Temnopleuroida 1.97 1.08 1.29 1.07 0.15 3.04 1.19 0.97 Arbaciapunctulata 2.17 0.33 0.19 Asteroidea 1.61 0.22 0.19 Ophiuroidea 0.14 0.49 0.65 0.12 Echinoidea 0.10 0.10 0.19 0.33 0.07 Arbacioida 0.10 0.01 Fish ..  :-_

Engraulidae** 18.74 10.22 0.38 2.85 A nchoa cubana** 23.71 4.76 0.95 2.84 Umbrina coroides** 4.76 20.25 0.39 3.02 0.30 0.56 0.61 1.71 2.28 1.28 2.70 Stellifer lanceolatus 3.21 7.14 2.85 1.32 Chloroscombruschrysurus 2.38 1.50 0.10 0.14 3.08 0.15 3.23 0.46 0.16 1.05 Haemulon aurolineatumi 5.49 0.10 1.90 0.73 Harengulajaguana*

• 6.42 0.60 0.67 Eucinostomus gula 3.52 0.30 0.95 0.47 Citharichthysmacrops 0.72 0.99 0.10 0.15 1.99 0.32 0.42

SLI SL2 SL3 Taxa Summer Winter Summer Winter IPeJost Summer Winter Total' Pre Post Pre IPost Pre Post Pre IPost Pre Ps Prionotusscitulus** 0.21 0.14 0.20 0.89 0.14 0.29 1.33 0.87 0.40 Anchoa sp.** 0.54 0.61 1.82 0.16 0.21 Ophidion sp. 0.52 1.14 0.11 0.18 Menticirrhuslittoralis** 0.95 0.10 0.10 0.28 0.30 0.09 0.16 0.15 Corvula sanctaeluciae 0.21 0.20 0.86 0.38 0.14 Lutjanus synagris 0.52 0.14 0.10 0.66 0.14 Micropogoniasundulatus 0.10 0.10 0.39 0.76 0.14 Bothuts robinsi 0.99 0.19 0.11 0.13 Selene setapinnis 0.21 0.14 4 0.40 0.47 0.13 Ophi__ion holbrookii 0.10 0.10 0.30 0.76 0.12 Ophidion grayi 0.95 0.10 Cvnoscion nothus** 0.10 0.40 0.38 0.09 Eucinostomusjonesii 0.93 0.09 Larinnisfasciatus 1.09 0.14 0.09 Leiostomus xanthurus** 0.59 0.29 0.09 Synodusfoetens 0.39 0.10 0.43 0.09 Sardinellaaurita** 0.10 0.69 0.08 Selene vomer 0.41 0.20 0.09 0.16 0.08 Stephanolepis hispida 0.41 0.41 0.10 0.08 Lagodon rhoinboides 0.20 0.19 0.09 0.05 Caranx crysos 0.20 0.15 0.09 0.04 Centropristisstriata 0.20 0.19 0.04

SL1 SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total' Pre I Post P o r _os Pre J Post Pre Post Posost Pre Opisthonema oglinum** 0.10 0.30 0.04 Eucinostomus harengulus 0.10 0.30 0.03 Labrisomus nuchipinnis 0.30 0.03 Prionotusrubio 0.10 0.19 0.03 Sciaenidae 0.10 0.19 0.03 Scorpaena brasiliensis 0.10 0.19 0.03 Achirus lineatus 0.10 0.09 0.02 Anchoa hepsetus** 0.14 0.14 0.02 Anchoa nmitchilli** 0.10 0.14 0.02 Caranxhippos 0.20 0.02 Cynoscion regalis 0.10 0.10 0.02 Decapteruspunctatus 0.20 0.02 Diplectrumformosum 0.10 0.09 0.02 Monacanthus ciliatus 0.28 0.02 Peprilusparu 0.10 0.10 0.02 Symphurus diomedeanus 0.10 0.09 0.02 Trichiurus lepturus 0.10 0.10 0.02 Anchoa lyolepis** 1 0.16 0.01 Anisotremus surinamensis 0.10 0.01 Anisotremus virginicus 0.10 0.01 Apogon binotatus 0.09 0.01 Ariopsisfelis 1 0.14 [7 0.01

SL SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total' Pre Post Pre [Post Pre [Post Pre IPost Pre [ Post Pre ] Post Caranxlatus 0.16 0.01 Centropristisphiladelphica 0.10 0.01 Citharichthysspilopterus 0.10 0.01 Cynoscion nebulosus 0.10 0.01 Dactyloscopus crossotus 0.14 0.01 Diapterusauratus 0.10 0.01 Elops saurus 0.10 0.01 Gymnachirus melas 0.10 0.01 Haemulon inacrostomum 0.16 0.01 Haemulon sp. 0.10 0.01 Halichoeres caudalis 0.09 0.01 Hippocampus erectus 0.14 0.01 Labridae 0.10 0.01 Narcine bancroftii 1.00 0.01 Oligoplites saurus 0.10 0.01 Opistognathusrobinsi 0.10 0.01 Orthopristischrysoptera 0.10 0.01 Polydactylus virginicus 0.10 0.01 Prionotus sp. 0.11 0.01 Raja eglanteria 0.10 0.01 Scorpaena grandicornis 0.10 0.01 Serranidae I 1 0.09 0.01

SLI SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total' Pre [Post Prs ummr Pre [Post Pre Post Pre f Post Syngnathusffuscus 0.10 0.01 Trachinocephalusmyops 0.10 0.01 Trachinotus carolinus 0.10 0.01 Trinectes maculatus I I I _ 1 0.09 0.01 Total [126.601 54.77 121.76 115.80 1182.931 15.20 112.21[10.90 150.52 113.20 1 9.97 1 6.08 48.13 Distance towed all events and areas = 107.2 km

• Comimercially or Recreationally Important Decapod Crustaceans

• • Representative Important Species

Table 26. CPUE (Number Collected per Kilometer Trawled) for All Fish Captured by Trawl for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

Season Pre- or Post- Total SLII Distance 1 CPUE Total 1 SL2 T1 Distance CPUE Total S1L3 Distance 1Mean CPUE 1

CPUE Uprate Fish Trawled Fish Trawled Fish Trawled C Pre- 719 9.7 74.43 337 10.1 33.43 241 10.5 22.89 43.58 Summer Post- 187 7.4 25.42 11 7.2 1.53 32 6.6 4.85 10.60 Pre- 32 10.2 3.15 17 10.3 1.65 15 9.2 1.63 2.14 Winter Post- 32 7.0 4.60 15 6.6 2.27 17 6.3 2.72 3.20 Total 970 34.1 28.42 380 34.2 11.12 305 32.6 9.35 Mean 243 8.5 26.90 n 2395 85 2 8.5 9.72 j 76 8.2 J8.021 21.31

Table 27. CPUE (Number Collected per Kilometer Trawled) for RIS Captured by Trawl Within Each Area for All Events, by Pre-and Post-Uprate, and Season, St. Lucie Plant EPU. Clupeiformes Presented Separate from Other RIS.

Taxa Uiupelnormes _ _ _ _ "*" ...

Engraulidae 18.74 10.22 0.38 2.85 Anchoa cubana 23.71 4.76 0.95 2.84 Harengulajaguana 6.42 0.60 0.67 Anchoa sp. 0.54 0.61 1.82 0.16 0.21 Sardinellaaurita 0.10 0.69 0.08 Opisthoneinaoglinum 0.10 0.30 0.04 Anchoa hepsetus 0.14 0.14 0.02 Anchoa mitchilli 0.10 0.14 0.02 Anchoa lyolepis 0.16 0.01 CPUE Clupeiformes 149.17 10.82 0.00 0.14 116.57 0.00 I0.00 0.61 1.33 11.82 0.00I 0.32 J 6.75 Other RIS. "... . , . . _. ..

Umbrina coroides 4.76 20.25 0.39 3.02 0.30 0.56 0.61 1.71 2.28 1.28 2.70 Prionotusscitulus 0.21 0.14 0.20 0.89 0.14 0.29 1.33 0.87 0.40 Menticirrhuslittoralis 0.95 0.10 0.10 0.28 0.30 0.09 0.16 0.15 Micropogoniasundulatus 0.10 0.10 0.39 0.76 0.14 Cynoscion nothus 0.10 0.40 0.38 0.09 Leiostom us xanthurus 0.59 0.29 0.09 Orthopristischrvsoptera 0.10 0.01 Trachinotus carolinus 0.10 0.01 CPUE Other RIS 5.07 ]21.34 [1.58 J3.02 1.79 [ 0.98 10.97 [0.91 4.27 2.28 J0.87 1.44 3.58 CPUE All RIS j54.24 22.15 1.58 13.16 18.35 10.98 10.97 1.51 15.60 14.10 10.87 1.76 10.33 Distance towed all events and areas = 100.9 km

Table 28. Number of RIS Specimens Captured by Trawl for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SL1 SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total Pre JPost Pre IPost Pre J Post Pre Post Pre ]Post Pre JPost Engraulidae 181 103 4 288 Anchoa cubana 229 48 10 287 Unmbrina coroides 46 149 4 21 3 4 4 18 15 8 272 Harengulajaguana 62 6 68 Prionotusscitulus 2 1 2 9 1 3 14 8 40 Anchoa sp. 4 4 12 1 21 Menticirrhuslittoralis 7 1 1 2 2 1 1 15 Micropogoniasundulatus I 1 4 8 14 Cynoscion nothus 1 4 4 9 Leiostomus xanthurus 6 3 9 Sardinellaaurita 1 7 8 Opisthonema oglinum 1 3 4 Anchoa hepsetus 1 2 Anchoa mitchilli 1 1 2 Anchoa lyolepis 1 1 Orthopristischrysoptera 1 1 Trachinotuscarolinus 1 1 Total [524 1163 J16 122[18517[10I 10 59 2 1 14

Table 29. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Catch Per Unit Effort (CPUE) Among Areas for RIS Fish Captured by Trawl During All Events, Seasons, Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Clupeiformes 8.71 3.79 0.86 All Events 2 126 1.2813 0.5269

+42.08 +/- 16.42 +/-4.36 14.92 6.38 0.21 Pre-uprate 2 72 0.9205 0.6284

+/-_55.33 +/- 21.52 +/- 0.83 0.44 0.33 1.72 Post-uprate 2 54 0.9472 0.6228

+/- 1.42 +1.41 +/- 6.60 15.21 6.38 1.38 Summer 2 72 1.7039 0.4266

+/-55.26 +21.52 +/- 5.73 0.06 0.33 0.17 Winter 2 54 0.0028 0.9986

+/-0.24 -- 1.41 +/- 0.71 Cynoscion nothus All Events 2 126 0.4231 0.8093 0.08 0.25 0.21 Pre-uprate 2 72 0.4416 0.8019

+/- 0.41 +/-_0.90 +/-_0.83 0.00 0.00 0.00 Post-uprate 2 54 0.0000 1.0000

+/- 0.00 +/- 0.00 +/- 0.00 0.08 0.25 0.21 Summer 2 72 0.4416 0.8019

+/- 0.41 +/- 0.90 - 0.83 0.00 0.00 0.00 Winter 2 54 0.0000 1.0000

+/- 0.00 +/- 0.00 + 0.00

Leiostomus xanthurus 0.12 0.05 0.00 All Events 2 126 2.0322 0.3620

+ 0.63 +/- 0.31 + 0.00 0.21 0.08 0.00 Pre-uprate 2 72 2.0569 0.3576

+/- 0.83 + 0.41 +/- 0.00 0.00 0.00 0.00 Post-uprate 2 54 0.0000 1.0000

+/- 0.00 +_0.00 1:0.00 0.00 0.00 0.00 Summer 2 72 0.0000 1.0000

+/- 0.00 +_0.00 -- 0.00 Winter 0.28 0.11 0.00 2 54 2.0764 0.3541

+ 0.96 +/- 0.47 +/- 0.00 Menticirrhuslittoralis 0.21 0.19 0.05 All Events 2 126 0.3456 0.8413

+/-_1.24 -- 0.71 +/- 0.22 _

0.04 0.13 0.04 Pre-uprate 2 72 0.0012 0.9994

+/- 0.20 +_0.61 +_0.20 0.44 0.28 0.06 Post-uprate 2 54 0.5339 0.7657 1 1.89 +/- 0.83 +/- 0.24 0.33 0.21 0.04 Summer 2 72 0.5242 0.7694

+/- 1.63 +/-_0.72 +/-_0.20 0.06 0. 17 0.06 Winter 2 54 0.0021 0.9989

+ 0.24 +/-_0.71 +/-_0.24

Micropogonias undulatus 0.02 0.29 0.24 All Events 2 126 0.4432 0.8012

+ 0.15 +/- 1.45 +/- 1.39 0.04 0.50 0.42 Pre-uprate 2 72 0.4784 0.7873

+/- 0.20 +/- 1.91 +/- 1.84 0.00 0.00 0.00 Post-uprate 2 54 0.0000 1.0000

+/- 0.00 +/- 0.00 +/- 0.00 0.00 0.38 0.42 Summer 2 72 2.0290 0.3626

+/-_0.00 +/-_1.84 +/-_1.84 0.06 0.17 0.00 Winter 2 54 1.0199 0.6005

+ 0.24 +_0.71 +/- 0.00 i Orthopristischrysoptera 0.02 0.00 0.00 All Events 2 126 2.0000 0.3679

+/- 0.15 +/- 0.00 +/- 0.00 0.04 0.00 0.00 Pre-uprate 2 72 2.0000 0.3679

+/- 0.20 +/- 0.00 +/- 0.00 0.00 0.00 0.00 Post-uprate 2 54 0.0000 1.0000

+/- 0.00 - 0.00 + 0.00 0.00 0.00 0.00 Summer 2 72 0.0000 1.0000

+/-_0.00 +/-_0.00 +/-_0.00 _____

Winter 0.6 00 .02 54 2.0000 0.3679

________ +/-0.24

+ +/-_0.00 +/-_0.00 _______________ _____

Prionotus scitulus All Events 2 126 3.8297 0.1474

+/- 1.01 +/- 1.06 +/- 1.78 0.33 0.75 1.17 Pre-uprate 2 72 5.5083 0.0637

+/- 1.27 +/- 1.33 +/- 2.24 0.11 0.06 0.00 Post-uprate 2 54 1.0199 0.6005

+/- 0.47 +/- 0.24 +/- 0.00 0.33 0.67 0.79 Summer 2 72 2.2054 0.3320

+/- 1.63 +/- 1.34 +/- 2.19 Winter 0.11 0.17 0.50 2 54 2.1336 0.3441

+/- 0.47 +/- 0.71 +/- 1.04 0.3441 Trachinotuscarolinus All Events 0.02 0.00 0.00 2 126 2.0000 0.3679 0.04 0.00 0.00 Pre-uprate 0.04 0.00 0.00 2 72 2.0000 0.3679

+/- 0.20 +/- 0.00 +/- 0.00 ____

Post-uprate 2 54 0.0000 1.0000

+/- 0.00 +/- 0.00 +/- 0.00 Summer 0.00 0.00 0.00 2 72 0.0000 1.0000

+/- 0.00 +/- 0.00 +/- 0.00 0.06 0.00 0.00 Winter 2 54 2.0000 0.3679

+/- 0.24 +/- 0.00 +/- 0.00 Umbrina coroides All Events 6.57 0.36 1.71 2 126 3.2054 0.2014

+/- 25.65 +/- 1.19 -5.50 2.92 0.21 1.13 Pre-uprate +/- 13.24 +/- 0.72 +/- 2.66 2 72 1.8080 0.4049 11.44 0.56 2.50 Post-uprate 11.44 0.6 2.50 2 54 4.4220 0.1096

+/- 36.13 +/- 1.62 +/- 7.88 _______________

10.08 0.42 2.42 Summer +/-3.54

+/-: 33.54 +/-.2

+/- 1.21 +/- 6.83 6.83 2 72 1.3863 0.5000 1.89 0.28 0.78 Winter +/- 4.9 4.90 +/-.18

+/- 1.18 +/- 2.8 2.84 2 54 3.5569 0.1689

Table 30. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Catch Per Unit Effort (CPUE) Among Pre- and Post-Uprate Events Within Each Area for RIS Fish Captured by Trawl, St. Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Clupeiformes 14.92 0.44 SLI 1 42 0.0383 0.8448

• 55.33 +/- 1.42 6.38 0.33 SL2 1 42 0.6778 0.4103

• - 21.52 +/- 1.41 0.21 1.72 SL3 1 42 0.1220 0.7269 Cynoscion nothus S~~i -0.083 0.600140750 036 SLI 1 42 0.7500 0.3865 0.25

-+/-0.41 +/-0.00 0.00 0.21 0.00 SL3 1 42 1.5366 0.2151 0.83 0.00 Leiostomus xanthurus SLI 1 42 1.5366 0.2151

+/- 0.83 +/- 0.00

.0.08 0.00 SL2 1 42 0.7500 0.3865

+/- 0.41 +/- 0.00 0.00 0.00 SL3 1 42 0.0000 1.0000

_+/- 0.00 +/- 0.00

Menticirrhus littoralis SLI *.04 0.4411 42 0.0581 0.8095

+/-+0.20 +/- 1.89 ________ ___ ____

0.13 0.28 SL2 1 42 0.6814 0.4091

• 0.61 +/- 0.83 0.04 0.06 SL3 1 42 0.0427 0.8363 Micropogonias undulatuis 0.04 0.00 SL1 1 42 0.7500 0.3865

+/- 0.20 +/- 0.00 0.50 0.00 SL2 1 42 1.5366 0.2151

+/- 1.91 +/- 0.00 0.42 0.00 SL3 1 42 1.5366 0.2151 0.20 14+ - 0.00 Orthopristischrysoptera SL2 1 42 0.7500 0.3865

+/-0.00 0.20 +/-0.00 0.00 SL2 000.0142 0.0000 1.0000

+/-0.00 0.00 +/-0.00 0.00 0.00 0.00 SL3 42 0.0000 1.0000

+ 0.00 +/-_0.00

Prionotusscitulus 0.33 0. 11 SLI 1 42 0.1369 0.7114

+/-1.27 +/- 0.47 0.75 SL2 + 1.33 0.06 1 42 4.9080 0.0267

+/- 0.24 1.17 SL3 + 2.24 0.00 1 42 8.2358 0.0041 L +0.00 Trachinotus carolinus SLI 0.00 1 42 0.7500 0.3865 0.00

+/- 0.20 + 0.00 SL2 1 42 0.0000 1.0000

+0.00 0.00 0.00 +/- 0.00 SUL.03.0 42 0.0000 1.0000

+0.00 -- 0.00

/nnn~

Umbrina coroides 2.92 11.44 SLI 1 42 3.6328 0.0567

+13.24 +36.13 ____ ________

SL2 0.21 0.56  ! 42 0.1594 0.6897

+/- 0.72 + 1.62 S1.13 2.50 1 42 0.0581 0.8095 SL3 *+/-_2.66 +/- 7.88

Table 31. CPUE (Number Collected per Kilometer Trawled) for RIS Captured by Trawl for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

Pre- or SL[ SL2 SL3 Mean Post- Number Distance CNumber Distance CNumber Distance CPUE CPUE Uprate Capture Trawled I I J I CPUldCapture Trawled _________II Pre- 524 9.7 54.24 185 10.1 18.35 59 10.5 5.60 26.07 Post- 163 7.4 22.15 7 7.2 0.98 27 6.6 4.10 9.07 Pre- 16 10.2 1.58 10 10.3 0.97 8 9.2 0.87 1.14 Post- 22 7.0 3.16 10 6.6 1.52 11 6.3 1.76 2.15 I Total Mean

.4 725 181 4

34.1 8.5 4

21.24 20.28 I-212 53 1

34.2 8.5 4

6.20 5.45 I-105 26 4

32.6 8.2

+

3.22 3.08 10.33

Table 32. Mean Total Length (mm) of RIS Captured by Trawl, for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SLU SL2 SL3 Mean Total Taxa Summer Winter Summer Winter Summer Winter Length Pre-] Post- Pre-I Post- Pr ost-Pre- Post- Pre- J tPost-Pr Post- (mm)

Micropogonias undulatus 248.00 234.00 241.50 234.38 237.36 Orthopristischrysoptera 230.00 230.00 Cynoscion nothus 226.00 225.00 225.75 225.44 Leiostomus xanthurus 190.17 191.50 190.50 Trachinotus carolinus 149.00 149.00 Prionotusscitulus 19.75 193.00 193.50 83.44 112.00 190.00 155.50 184.25 142.59 Harengulajaguana 126.97 120.33 125.92 Opisthonema oglinumn 115.00 98.00 102.25 Sardinellaaurita 160.00 82.14 91.88 Menticirrhuslittoralis 68.00 227.00 52.45 77.20 171.00 102.70 88.29 Umbrinacoroides 79.04 59.66 147.00 54.90 55.00 72.10 34.75 90.10 54.95 42.45 66.20 Anchoa lyolepis 1 56.40 56.40 Anchoa cubana 54.69 57.33 42.56 54.47 Anchoa hepsetus 59.20 45.80 52.50 Anchoa mitchilli 50.00 53.10 51.55 Engraulidae 38.68 34.80 34.00 36.07 Anchoa sp. 20.55 43.80 _ j 23.24 38.90 24.66 Mean TL (mm) 173.79 60.12 185.63 54.49 62.70 172.19 1213.221 48.17 [131.791 40.86 184.251 48.87 [ 80.20

Table 33. Mean Weight (g) of RIS Captured by Trawl Within Each Area for All Events, by Pre- and Post- Uprate, and Season, St.

Lucie Plant EPU.

SLU SL2 SL3 Total Taxa Summer Winter Summer Winter Summer Winter Mean Pre- JPost- Pre- Post- Pre- Post- Pre-f Post- Pre- J Post- Pre- [Post- Weight (g)

Orthopristischrysoptera 160.0 160.0 Micropogonias undulatus 167.0 150.0 168.5 139.9 150.7 Cynoscion nothus 137.9 132.5 151.0 141.3 Leiostornus xanthurus 78.7 79.6 79.0 Trachinotus carolimnus 45.3 45.3 Prionotusscitulus 38.0 55.6 53.0 15.2 11.5 50.9 50.4 55.3 42.2 Harengulajaguana 18.8 18.4 18.7 Menticirrhuslittoralis 3.2 113.0 1.5 4.0 54.4 15.4 Sardinellaaurita 31.6 7.9 10.9 Opisthonernaoglinum 14.9 9.4 10.8 Umbrina coroides 10.2 2.8 57.8 2.9 3.6 4.8 2.6 10.9 2.0 0.9 6.3 Anchoa lyolepis 1.1 1.1 Anchoa cubana 1.1 1.2 0.4 1.1 Anchoa hepsetus 1.5 0.4 1.0 Anchoa mitchilli 0.01 1.1 0.6 Engraulidae 0.3 0.2 0.1 0.3 Anchoa sp. 0.1 _ 0.2 0.4 0.1 0.3 0.1 Mean Weight (g) 18.2 13.5 80.9 [1.4 19.3 4.8 1106.6 1 2.7 148.8 1.1 55.3 J 0.9 [ 15.6

Table 34. Calculated and Mean Biomass (g) for RIS Captured by Trawl Within Each Area for All Events, by Pre- and Post-Uprate, and Season, St. Lucie Plant EPU. Clupeiformes Presented Separate from Other RIS.

SLU SL2 SL3 Summer Winter Summer Winter Summer Winter Biomass Taxa P(g)

P -- F -[-Pos P Pe Pot Pre [Post Pre ost Pre J Post Pre [Post Pre J Post Pre ] Post Micropogoniasundulatus 167.0 150.0 674.1 1119.0 2110.1 Umbrinacoroides 471.1 423.2 231.1 60.0 10.9 19.1 51.6 196.1 29.7 7.2 1716.6 Prionotusscitulis 76.0 55.6 106.0 136.5 11.5 152.7 705.6 442.5 1686.4 Harengulajaguana 1165.2 110.1 1273.2 Cvnoscion nothus 137.9 530.1 603.8 1271.8 Leiostonus xanthurus 472.1 238.8 710.9 Anchoa cubana 252.7 48.0 4.0 310.2 Menticirrhus littoralis 22.5 113.0 3.0 7.9 54.4 231.7 Orthopristischrysoptera 160.0 160.0 Sardinellaaurita 31.6 55.2 86.8 Engraulidae 60.1 25.1 0.1 77.3 Trachinotus carolinus 45.3 45.3 Opisthonemaoglinum 14.9 28.1 43.0 Anchoa sp. 0.2 1.6 0.6 0.3 2.6 Anchoa hepsetus 1.5 0.4 1.9 Anchoa lyolepis 1.1 1.1 Anchoa mitchilli 0.01 1.1 1 1 1.1 Mean Biomass (g) 1245.51 84.0 1 184.9 [ 30.2 121.6 11.2 I 355.2 20.4 I383.3 ' 15.2 442.5 2.2 572.4

Table 35. CPUE (Number Collected per Kilometer Trawled) for All Invertebrates Captured by Trawl for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

Pre- or SLU SL2 SL3 1 Season Post- Number Distance Number Distance Number Distance Uprate Capture Trawled CPUE Capture Trawled CPUE Capture Trawled CPUE CPUE Pre- 504 9.7 52.17 1507 10.1 149.50 291 10.5 27.64 76.44 Summer Post- 216 7.4 29.35 98 7.2 13.67 55 6.6 8.35 17.12 Winter Pre- 189 10.2 18.62 109 10.3 10.56 77 9.2 8.34 12.51 Post- 76 7.0 10.92 56 6.6 8.48 21 6.3 3.36 7.59 Total 985 34.1 28.86 1770 34.2 51.80 444 32.6 13.62 Mean Mean _

246 246_ 8.5 27.77 2 443 j 8.5 45.55 111 I 8.2 8.26 1.9 1.92J 31.70

Table 36. CPUE (Number Collected per Kilometer Trawled) for CRI Decapod Crustaceans Captured by Trawl for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SLU SL2 SL3 Season Pre- Post. Distance Number Number Distance Mean Uprate

_____ _Capture [Trawled CPUDstnc rawled ICPUE Capture ICPUE CprI____ Trawled ____

CPUE Summer 61 9.7 6.31 45 10.1 4.46 72 10.5 6.84 5.87 Pre Winter 10 7.4 1.36 27 7.2 3.77 28 6.6 4.25 3.12 Summer 10 10.2 0.99 14 10.3 1.36 14 9.2 1.52 1.29 Post Winter 1 36 7.0 5.17 20 6.6 3.03 5 6.3 0.80 3.00 Total 117 34.1 3.43 106 34.2 3.10 119 32.6 3.65 Mean J29 8.5 3.461271 n 8.5

1. 3.15130 8.2 3.65 3.35

Table 37. Total Number of Fish and Invertebrates Captured by Gill Netting for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SLI SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Grand

~ Total Pre Post Pre Post Pre Pos Post Pe Pre Pr Post T M,o. lusc Cephalopoda JJ] II I IT 1[

Tollusc Total I I I 1 _ 1

__1I]

_ _i L 1 Crustacean Arenaeus cribrarius* 4 2 1 6 1 1 15 Calappaflarnmea _ __ 1 3 2 6 Portunusspinirmanus 4 2 6 Calappoidea 1 2 1 4 Paguroidea ____ ___ ___ ____ ___ ___ ___ I___ 1 2 Portunus sp. j 1 1 Crustacean Total ___ __ 159 . 1 J9 5I 3 34[

E.chi~ncodprma-ta ___ ______

Temnopleuroida 16 1 15 5 4 7 48 Arbacia punctulata 20 1 2 23 Clypeasteroida 2 1 1 2 6 Asteroidea 1 1 3 Echinodermata 2 1 3 Astropecten sp. 1 1 chinooermata 20

[otal 20 I2 0 116 2 6 18 0 I 2t 8 0 84 Fishov 472536 Rhizoprionodonterraenovae 46 19 74 53 22 3 51 50 28 43 272 103 764

SL1 SL2 SL3 Summer Winter Summer Winter Summer Winter Toal Taxa FTotal

---

r ---

Pre Post Pre J Post Pre j Post Pre ] Post Pre J Post Pre J Post Chloroscombruschrvsurus 115 12 21 15 77 327 12 91 5 84 759 Sphyrna tiburo 64 246 70 14 49 13 97 102 83 738 Scomberoinorus maculatus** 37 34 17 41 1 11 100 2 15 258 Micropogoniasundulatus** 45 2 2 72 2 83 14 220 Bagre marinus 36 2 29 25 119 1 212 Caranx crysos 14 6 10 6 17 12 20 2 26 38 13 8 172 Larimusfasciatus 32 46 67 145 Caranx latus 35 1 61 5 30 132 Pomatomus saltatrix** 31 25 2 4 7 2 27 3 101 Leiostomnus xanthurus** 10 74 2 11 1 98 Brevoortiasmithi** 19 5 6 5 2 17 43 97 Cynoscion nothus** 61 33 2 96 Carcharhinusbrevipinna 25 3 2 18 10 22 1 4 85 Caranx hippos 1 12 27 23 9 4 76 Menticirrhusamnericanus** 11 2 3 4 26 2 13 61 Lutyanus synagris 1 I1 1 35 2 50 Synodusfoetens 2 2 7 1 1 2 26 3 44 Trichiuruslepturus 17 1 3 10 1 6 4 1 43 Elops saurus 24 5 6 35 Orthopristischtysoptera** 8 3 1 1 1 3 11 28 Trachinotus carolinus** 4 15 3 1 23 Selene setapinnis 17 1 2 1 1 22

SLI SL2 SL3 Taxa Summer Winter

___ __ __TSummer

[Pot __J Pst 1______

Winter Summer Winter__i rand Toal

--FPost Total Pre__ Post Pre Post Pre __Post Pre J Post Pre J Post Anisotremus virginicus 13 5 1 19 Centropristisstriata 4 1 6 3 1 1 16 Archosargusprobatocephalus 11 1 1 1 1 15 Carcharhinusacronotus 1 1 1 6 2 1 2 1 15 Prionotusscitulus** 2 1 1 1 7 1 13 Cynoscion regalis 7 1 1 1 10 Menticirrhussaxatilis** 4 3 2 9 Citharichthysmacrops 2 1 1 4 8 Rachycentron canadurn 2 2 1 3 8 Umbrina coroides** 7 7 Diplodus holbrookii 5 1 6 Carcharhinuslimbatus 1 1 1 1 1 5 Calaniusarctifirons 2 2 4 Rhinobatos lentiginosus 1 1 1 1 4 Brevoortiatyrannus ** 3 3 Echeneis naucrates  ? 1 3 Menticirrhuslittoralis** 2 1 3 Mustelus canis 3 3 Paralichthysalbigutta 1 2 3 Peprilusparu 2 1 3 Prionotusrubio 3 3 Prionotus tribulus 3 3

SLP SL2 SL3 Winter Summer Winter Summer Winter Toal Taxa Summer Post Pre

_ _ Post _Pre Post Pre Post T t Pre [ Post _Pre _ Post Pre Scombridae 3 3 Centropornus undecimalis 2 2 Chaetodipterusfaber 1 1 2 Corvula sanctaeluciae 1 1 2 Myliobatis goodei 2 2 Stephanolepis hispida 2 2 Trachinotusfalcatus 1 1 2 Acanthostracionquadricornis 1 1 Aetobatus narinari 1 1 Albula vulpes 1 1 Aluterus mnonoceros 1 1 Ancylopsetta ommata 1 1 Archosargus rhomboidalis 1 1 Citharichthysspilopterus 1 1 Diapterus auratus 1 1 Diplectrumformosum 1 1 Euthvnnus alletteratus 1 1 Haemulon aurolineaturn 1 1 Hippocampus erectus 1 1 Lagodon rhomboides 1 1 Lutyanus analis 1 1 Luqanus griseus 1 1

SL1 SL2 SL3 Summer Winter Summer Winter Summer Winter Toal Taxa Total Pre ] Post Pre Post Pre7 Post Pre J Post Pre [Post Pre Post Membras martinica 1 1 Opisthonema oglinum** 1 1 Opsanus beta 1 1 Peprilus burti 1 Pseudocaranxdentex 1 Raja eglanteria 1 1 Sciaenops ocellatus 1 Scorpaenaplum ieri 1 Selene vomer 1 1 Fish Total 692 300 287 155 602 16 451 287 809 108 615 139 4461 Total All Organisms 712 321 289 156 623 27 458 288 826 115 626 139 14580

• Commercially or Recreationally Important Decapod Crustaceans

• • Representative Important Species

Table 38. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Number of Taxa, Catch Per Unit Effort (CPUE), and Biomass Among Areas by Sampling Period for Fish Captured by Gill Net During All Events, Seasons, Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Fish Taxa All Events 45.14 3.91 -4.71 2 126 10.3531 0.0056

- 4.99 7.00 4.79 5.75 Pre-uprate 2 72 3.5435 0.1700

+/- 6.21 +5.32 +/-5.58 4.28 2.50 Post-uprate + 2.68 2.72 + 2.18 2 54 9.5574 0.0084

+/- 4.38 6.54 5.08 Summer +/- 6.27 4.00 +/- 5.23 2 72 7.0597 0.0293

+/-_5.51 4.89 3.78 3.39 Winter 2 54 4.7619 0.0925

_-2.99 +/-4.36 +/-3.81 Fish CPUE All Events 2 126 0.5731 0.0570

E3.28 +/-44.53 +/- 3.98 Pre-uprate 3.432 .8272 1.8568 0.3952

+/- 3.01 +/- 5.36 +/- 4.71 2.41 1.25 1.60 Post-uprate 2 54 4.5163 0.1045

-+/-3.60 +/- 2.84 +/- 2.05 3.36 3.20 Summer +/- 3.87 1.70 +/- 4.01 2 72 6.2598 0.0437

+/- 2.64 2.65 3.36 2.91 Winter 2 54 1.1617 0.5594

+ 2.34 +/-6.19 +/-4.05

RIS Fish Taxa 1.57 1.12 1.10 All Events 2 126 2.1303 0.3447

+/- 2.15 +/-2.07 +/- 2.06 2.08 1.46 1.71 Pre-uprate 2 72 0.6388 0.7266

+ 2.54 +/- 2.30 +/- 2.54 0.89 0.67 0.28 Post-uprate 2 54 2.9067 0.2338

________ + 1.28 + 1.68 +/- 0.46 _ _ _ _ _ _ _ _ _ _ _ _

1.50 1.21 1.33 Summer 2 72 1.0456 0.5929

• 2.38 +/- 2.34 +/- 2.20 1.67 1.00 0.78 Winter 2 54 3.4161 0.1812

• 1.88 +/- 1.72 +/-1.87 RIS CPUE 0.70 0.50 0.64 All Events 2 126 1.2874 0.5253

+/-_1.10 +/- 1.15 +/-_1.31 1.05 0.68 1.04 Pre-uprate 2 72 0.9704 0.6156

+1.31 +/- 1.33 +/-1.61 0.23 0.27 0.12 Post-uprate 2 54 1.1589 0.5602

+/- 0.41 +/- 0.84 +/- 0.32 0.65 0.59 0.90 Summer 2 72 1.4533 0.4835

+/- 1.13 +/- 1.34 +/- 1.58 0.75 0.38 0.31 Winter 2 54 1.6824 0.4312

________ +/-_1.06 +/- 0.86 + 0.71 __ _ _ _ _ _ _ _ _ _ _ __ _ _ _ _

RIS Fish Biomass 3318.58 2573.51 4025.580.4620 5129.48 3662.32 6574.98 Pre-uprate 2 72 0.6932 0.7071

+7555.34 +/-7540.33 - 11713.08 904.05 1121.78 626.39 Post-uprate 2 54 2.0986 0.3502

• 1952.92 +/- 3468.57 +2052.17 Summer 3259.58 3291.07 5373.58 272 1.2857 0.5258

+/- 6955.74 +/- 7634.70 +/- 10908.06 3397.24 1616.78 2228.25 Winter 2554 2.9323 0.2308

_______+/- 5135.83 +/-3507.50 +/-6653.81 ___________________

Table 39. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Number of Taxa, Catch Per Unit Effort (CPUE), and Biomass Among Pre- and Post-Uprate Events Within Each Area for Fish Captured by Gill Net. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Fish Taxa 7.00 4.28 SLI 1 42 0.7346 0.3914

+6.21 S +/- 2.68 4.79 2.72 SL2 1 42 2.4941 0.1143

+/--5.32 4.38 5.75 SL3 5.58 2.50 1 42 3.8265 0.0504

-2.18 Fish CPUE 3.54 2.41 SLI 1 42 2.9006 0.0885

+/--3.01 +/- 3.60 3.29 1.25 SL2 1 42 2.7424 0.0977

+_5.36 +/- 2.84 4.18 1.60 SL3 1 42 2.4435 0.1180 4-4.71 +/- 2.05 RIS Fish Taxa Area a Pr-pae Potutt 2.08 0.89 SLI 1 42 0.9911 0.3195

+/- 2.54 +/- 1.28 1.46 0.67 SL2 1 42 1.1158 0.2908

- 2.30 - 1.68 1.71 0.28 SL3 1 42 3.5728 0.0587

+/- 2.54 +/--0.46 _

1.35 +/-0.41 SL2 0.68 0.27 1 42 1.2699 0.2598

_- 1.33 +/- 0.84 1.04 0.12 SL3 1 42 3.0457 0.0810 1.61 4600.32 RIS Fish Biomass

+/-3755.34 19521.92 SL23662.32 1121.78 1 42 1.4071 0.2355 4+/- 7540.33 +/- 3468.57 6574.98 626.39 SL3 1 42 3.3522 0.0671 4- 11713.08 +/- 2052.17

Table 40. CPUE (Number Collected Per Hour of Soak Time) for All Taxa Captured by Gill Netting for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SL1 SL2 SUL3 Winter Summer Winter Summer Winter Toal Taxa Summer Pre Post jP¶j st- Pr Pos Pre Post Prie Post ToGrand Mollusc ___

Cephalopoda I IiI X I Z IZiZ 0.12I IZII I 0.01 Crustacean Arenaeus cribrarius 0.25 0.32 0.13 0.39 0.16 0.10 0.13 Calappaflammea 0.14 0.48 0.31 0.05 Portunusspinimanus 0.65 0.31 0.05 Calappoidea 0.06 0.13 0.10 0.04 Paguroidea 0.06 0.10 0.02 Portunus sp. 0.14 0.01 Total 0.14 J 0.14 0.32 1.45 0.13_0.58 0.78 0.31 [_0.30 Echinodermata Temnopleuroida 1.01 0.13 0.95 0.67 0.26 0.73 0.42 Arbaciapunctulata 2.73 0.16 0.31 0.20 Clypeasteroida 0.13 0.06 0.13 0.13 0.05 Asteroidea 0.13 0.06 0.10 0.03 Echinodermata 0.13 0.06 0.03 Astropecten sp. 1 0.16 0.01 Total 1.26 2.73 0.25 11.01 0.32_ 0.80 [0.52 0.31 0.83 0.74 Rhizoprionodon terraenovae 2.90 2.60 9.31 7.55 1.39 0.48 6.78 6.24 1.81 6.67 28.19 16.22 6.72 Chloroscombruschrysurus 7.24 1.64 2.64 2.14 4.86 1 43.50 1.50 5.88 0.78 8.70 6.68

SLI SL2 SL3 Grand Toal Summer Winter Summer Winter Summer Winter Taxa I Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Sphyrna tiburo 4.03 33.62 8.81 2.00 3.09 1.73 12.10 6.59 8.60 6.49 Scomberomorus maculatus* 2.33 4.28 2.42 2.59 0.13 1.37 6.46 0.31 2.36 2.27 Micropogoniasundulatus* 2.83 0.25 0.29 4.54 0.25 5.36 1.45 1.94 Bagre marinus 2.27 0.29 1.83 3.12 7.69 0.16 1.86 Caranx crysos 0.88 0.82 1.26 0.86 1.07 1.94 2.66 0.25 1.68 5.89 1.35 1.26 1.51 Larimusfasciatus 2.01 2.90 4.33 1.28 Caranx latus 2.20 0.13 3.85 0.62 1.94 1.16 Poinatoniussaltatrix* 1.95 3.14 0.29 0.25 0.93 0.25 1.74 0.31 0.89 Leiostomus xanthurus* 0.63 4.67 0.25 0.71 0.10 0.86 Brevoortia smithi* 2.39 0.71 0.38 0.67 0.25 1.10 4.46 0.85 Cynoscion nothus* 3.84 2.08 0.13 0.84 Carcharhinusbrevipinna 1.57 0.38 0.29 1.14 1.33 1.42 0.16 0.41 0.75 Caranx hippos 0.06 0.76 3.37 1.49 1.40 0.41 0.67 Menticirrhus americanus* 1.38 0.29 0.19 0.53 3.24 0.13 1.35 0.54 Lutyanus synagris 0.06 1.57 0.06 3.63 0.31 0.44 Synodusfoetens 0.13 0.27 0.88 0.14 0.06 0.27 2.69 0.47 0.39 Trichiurus lepturus 1.07 0.14 0.43 0.63 0.12 0.39 0.62 0.16 0.38 Elops saurus 1.51 0.32 0.39 0.31 Orthopristischrvsoptera* 0.50 0.38 0.14 0.06 0.12 0.19 1.14 0.25 Trachinotus carolinus 0.25 1.87 0.19 0.10 0.20 Selene setapinnis 1.07 0.06 0.13 0.16 0.10 0.19 Anisotremus virginicus 0.82 0.68 0.14 0.17

SL1 SL2 SL3 Summer Winter Summer Winter Summer Winter Grand Taxa Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Centropristisstriata 0.25 0.14 0.75 0.43 0. 13 0.06 0.14 Archosargusprobatocephalus 0.69 0.14 0.06 0.06 0.16 0.13 Carcharhinusacronotus 0.06 0.14 0.14 0.38 0.27 0.12 0.13 0.16 0.13 Prionotusscitulus* 0.13 0.14 0.06 0.12 0.45 0.16 0.11 Cynoscion regalis 0.44 0.14 0.06 0.06 0.09 Menticirrhussaxatilis* 0.25 0.19 0.13 0.08 Citharichthysnmacrops 0.13 0.14 0.06 0.26 0.07 Rachycentron canadum 0.29 0.25 0.06 0.31 0.07 Umbrina coroides* 0.44 0.06 Diplodus holbrookii 0.31 0.16 0.05 Carcharhinuslimbatus 0.06 0.14 0.12 0.06 0.16 0.04 Calamus arctifrons 0.13 0.29 0.04 Rhinobatos lentiginosus 0.14 0.14 0.10 0.16 0.04 Brevoortia tyrannus* 0.43 0.03 Echeneis naucrates 0.13 0.10 0.03 Menticirrhuslittoralis* 0.13 0.10 0.03 Mustelus canis 0.40 0.03 Paralichthysalbigutta 0.14 0.27 0.03 Peprilusparu 0.13 0.14 0.03 Prionotusrubio 0.19 0.03 Prionotustribulus 0.47 0.03 Scombridae 0.19 0.03

SL1 SL2 SL3 Summer Winter Summer Winter Summer Winter rand Taxa Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Centropomus undecimalis 0.13 0.02 Chaetodipterusfaber 0.06 0.12 0.02 Corvula sanctaeluciae 0.06 0.12 0.02 Myliobatis goodei 0.13 0.02 Stephanolepishispida 0. 13 0.02 Trachinotusfalcatus 0.06 0.06 0.02 Acanthostracionquadricornis 0.06 0.01 Aetobatuts narinari 0.06 0.01 Albula vulpes 0.16 0.01 Aluterus monoceros 0.16 0.01 Ancylopsetta ommata 0.10 0.01 Archosargus rhomboidalis 0.06 0.01 Citharichthysspilopterus 0.14 0.01 Diapterusauratus 0.06 0.01 Diplectrumformosum 0.13 0.01 Euthynnus alletteratus 0.06 0.01 Haeinulon aurolineatum 0.06 0.01 Hippocampus erectus 0.16 0.01 Lagodon rhomboides 0.14 0.01 Lut'anus analis 0.06 0.01 Lutjanus griseus 0.14 0.01 Membras martinica O.13 0.01

SL1 SL2 SL3 Summer Winter Summer Winter Summer Winter Grand Taxa Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Opisthonema oglinum* 0.14 0.01 Opsanus beta 0.14 0.01 Peprihesburti 0.13 0.01 Pseudocaranxdentex 0.06 0.01 Raja eglanteria 0.06 0.01 Sciaenops ocellatus 0.06 0.01 Scorpaenaplum ieri 0.13 0.01 Selene vomer 0.06 0.01 Total 43.57 41.00 36.10 22.09 37.98 2.58 60.00 35.80 52.25 16.74 63.73 21.89 39.24 Total 0.12 0.01 Gill Net Total 44.83 43.87 36.35 22.23 39.31 4.35 60.93 35.93 53.35 17.83 64.87 21.89 40.29

Table 41. Mean CPUE (Number Collected Per Hour of Soak Time) for All Fish Captured by Gill Netting for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

Season Pre- Post-Uprate I SLI I SL2 SL3 I Total Pre 43.57 37.98 52.25 44.54 Summer Post 41.00 2.58 16.74 21.24 Pre 36.10 60.00 63.73 53.87 Winter Post 22.09 35.80 21.89 27.17 Total 1 37.57 36.08 44.05 1 39.24

Table 42. Total Number of RIS Captured by Gill Netting for All Events, by Pre-and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

Season Uprate S SL1 L SL2 SL3 Total Pre 207 242 257 706 Summer Post 2 0 2 4 Pre 94 17 87 198 Winter Post 32 62 16 110 Total 335 321 362 1018

Table 43. Mean CPUE (Number Collected Per Hour of Soak Time) for RIS Captured by Gill Netting for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

Season Post-Uprate LS2 J UL2 L3 Total Pre 13.03 15.27 16.60 14.95 Summer Post 0.27 0.00 0.31 0.20 Pre 11.82 2.26 9.02 7.88 Winter Post 4.56 7.73 2.52 5.14 Total 8.78 8.54 9.54 8.95

Table 44. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Catch Per Unit Effort (CPUE) Among Areas for RIS of Fish Captured by Gill Net During All Events, Seasons, Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Clupeiformes e

Pi d0 L.0 0 .03 0 .10fn ,H All Events 0.8 00 .02 126 1.3985 0.4970

+/- 0.22 +/-0.11 +/- 0.37 0.08 0.04 0.17 Pre-uprate 2 72 0.3159 0.8539

+/- 0.25 + 0.14 +/-0.48 0.08 Post-uprate + 0.18 0.01 0.00 2 54 5.9022 0.0523

+/- 0.03 +/- 0.00 0.01 0.03 0.08 Summer 2 72 0.0016 0.9992

+/- 0.03 +/- 0.13 +/- 0.40 Winter 2 54 2.3144 0.3144

+/- 0.31 +/- 0.08 +/- 0.34 Cynoscion nothus 0.11 0.05 <0.012 All Events 0.1 00 .1 2126 2.0063 0.3667 0.19

+/-40.35 +/-0.09 0.25 <

+/- 0.01 0.02 0.19 0.09 < 0.01 Pre-uprate 2 72 2.2213 0.3293

+/- 0.46 +/- 0.33 +/- 0.02 0.00 0.00 0.00 Post-uprate 2 54 0.0000 1.0000

+/- 0.00 +/- 0.00 +/- 0.00 0.19 0.09 < 0.01 Summer 2 72 2.2213 0.3293

+/- 0.46 +/- 0.33 +/- 0.02 0.00 0.00 0.00 Winter 2 54 0.0000 1.0000

+/- 0.00 +/- 0.00 +/- 0.00

Leiostomus xanth urus 0.02 0.10 0.02 All Events 2 126 0.5639 0.7543

+/- 0.08 +/- 0.57 +/- 0.09 0.03 0.17 0.03 Pre-uprate 2 72 0.1861 0.9112

+/- 0.10 +/- 0.76 +/- 0.11 0.00 0.01 0.00 Post-uprate 2 54 2.0000 0.3679

+/- 0.00 +/- 0.03 +/- 0.00 0.03 0.17 0.03 Summer 2 72 0.2575 0.8792 0.10 +/-0.76 +/- 0.11 0.00 0.01 < 0.01 Winter 2 54 1.0199 0.6005 0.00 -- 0.03 +/- 0.01 Menticirrhus spp.

0.04 0.05 0.02 All Events 2 126 1.0223 0.5998

+/- 0.16 +/- 0.28 +/- 0.08 0.07 0.02 0.03 Pre-uprate 2 72 0.7491 0.6876

+/- 0.20 +/- 0.05 +/- 0.10 0.02 0.10 0.00 Post-uprate 2 54 1.0199 0.6005

+/- 0.07 +/- 0.42 +/- 0.00 0.02 0.01 0.01 Summer 2 72 0.2372 0.8881

+/- 0.05 +/- 0.04 +/- 0.04 0.08 0. 11 0.03 Winter 2 54 1.0204 0.6004

+/-_0.24 +/- 0.42 +/- 0.11

Micropogoniasundulatus All Events 2 126 1.7907 0.4085

+/- 0.25 +/- 0.33 +/- 0.44 0.15 0.14 0.25 Pre-uprate 2 72 0.7939 0.6724

+/- 0.32 +_0.43 +_0.56 l 0.02 0.01 0.00 Post-uprate 2 54 2.1562 0.3402

_______ +/- _0.05 +/-_0.03 +/-:_0.00 ___ _________

0.14 0.14 0.23 Summer 2 72 0.1982 0.9056

+/- 0.32 + 0.43 L 0.56 0.03 0.01 0.03 Winter 2 54 3.0613 0.2164

+0.06 +/-0.03 +/- 0.11 Orthopristischrysoptera 0.03 0.01 0.01 All Events 2 126 2.7048 0.2586

+/- 0.07 +/- 0.03 +/- 0.06 0.04 < 0.01 0.02 Pre-uprate 2 72 3.3289 0.1893

+ 0.09 +/- 0.01 +/-_0.08 0.01 0.01 0.00 Post-uprate 2 54 1.0199 0.6005

+/- 0.04 +/- 0.04 +/-_0.00 0.03 < 0.01 0.01 Summer 2 72 2.5278 0.2826

+/- 0.07 - 0.01 +/-_0.03 0.03 0.01 0.02 Winter 2 54 0.4741 0.7890 1+/-0.08 +/- 0.04 +/- 0.09 1

Pomatomus saltatrix 0.12 0.03 0.06 All Events 2 126 4.0038 0.1351

+/- 0.29 +/- 0.15 +/- 0.21 0.20 0.06 0.10 Pre-uprate 2 72 4.3014 0.1164

+ 0.36 +/- 0.20 +/- 0.28 0.02 0.01 0.00 Post-uprate 2 54 1.0199 0.6005

+/- 0.08 +/- 0.03 +/- 0.00 0.09 0.02 0.10 Summer 2 72 1.6403 0.4404

+ 0.23 +/- 0.06 +/- 0.28 0.16 0.06 0.01 Winter 2 54 4.1719 0.1242 0.1242

+/-_0.36 +/- 0.23 +/- 0.02 Prionotusscitulus 0.01 0.01 0.02 All Events 2 126 1.5055 0.4711

+/- 0.02 +/- 0.02 +/- 0.07 0.01 < 0.01 0.03 Pre-uprate 2 72 2.2038 0.3322

+/- 0.02 + 0.01  : 0.09 0.01 0.01 0.01 Post-uprate 0 2 54 0.0028 0.9986

+/- 0.03 +/- 0.04 +/- 0.03 0.01 < 0.01 0.03 Summer 2 72 2.0031 0.3673

+/- 0.03 +/- 0.01 +/- 0.09 Winter 0.00 0.01 0.01 2 54 1.0199 0.6005

+/- 0.00 + 0.04 +/- 0.03 1 1 1

Scomberomorus macudatus 0.20 0.10 0.26 All Events 2 126 0.9733 0.6147

+/- 0.40 +/- 0.23 +/- 0.76 0.26 0.12 0.38 Pre-uprate 2 72 0.7852 0.6753

+/- 0.48 -- 0.27 +_0.96 0.11 0.06 0.11 Post-uprate 2 54 0.1748 0.9163

+/- 0.25 +/- 0.16 +/- 0.32 0.12 0.12 0.39 Summer 2 72 0.7331 0.6931

+/- 0.35 +/- 0.27 +/- 0.96 0.30 0.07 0.09 Winter 2 54 5.4119 0.0668

+ 0.45 + 0.16 + 0.32 Trachinotuscarolinus 0.00 0.03 0.01 All Events 2 126 4.2321 0.1205

+/- 0.00 +/- 0.16 +/- 0.02 0.00 0.01 0.01 Pre-uprate 2 72 4.0433 0.1324

+/- 0.00 +/- 0.03 +/- 0.03 0.00 0.06 0.00 Post-uprate 0 2 54 2.0000 0.3679

+ 0.00 +/- 0.24 +/- 0.00 0.00 0.01 0.01 Summer 2 72 3.2212 0.1998

+/- 0.00 + 0.03 +/- 0.03 0.00 0.06 < 0.01 Winter 2 54 1.0199 0.6005

+/- 0.00 +/- 0.24 +/-+0.01

Umbrina coroides All Events q-0.01 0.1 0.00 00 +0.00

.02 126 2.0000 0.3679

+/-_0.08 +/-_0.00 +_0.00 ____ ____ ____

0.02 0.00 0.00 Pre-uprate 2 72 2.0000 0.3679 S0.011 0.00 0.00 Post-uprate 0.00 0.00 0.00 54 0.0000 1.0000

+/- 0.00 +/- 0.00 +/- 0.00 1 0.02 0.00 0.00 Summer 2 72 2.0000 0.3679

+/- 0.11 +/- 0.00 +/- 0.00 0.00 0.00 0.00 Winter 2 54 0.0000 1.0000

= 0.00 - 0.00 + 0.00

Table 45. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Catch Per Unit Effort (CPUE) Among Pre- and Post-Uprate Events Within Each Area for RIS of Fish Captured by Gill Net, St. Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

~Cueiformes~l SL1 + 0.33 -0.08 1 42 0.4430 0.5057

<0.01 0.01 SL2 +/--0.02 0.14 +/--0.00 0.03 1 42 0.5982 0.4393 SL3 0.19 010.0142 0.00 3.2267 0.072 4-0.48 4-0.00 1111 C, noscion nothus SLI ~0.19 0.00 14 .27 002 SL2 000.0142 2.3615 0.1244

• -0.33 +/- 0.00 SL3 <000.0142 0.7500 0.3865

• -0.02 -00 Leiostonms xanth urus pf n SL1 +/--0.13 0.10 +/--0.00 0.00 1 42 2.3615 0.1244 0.03 0.00 SL2 010.1142 1.1759 0.2782

+/- 0.76 +/-40.03 SL3 000.0142 3.2267 002

+/--0.11 + 0.00 I E JO O I Men tAcirrius s 0.07 0.02 SL1 +/--0.07 0.20 +/--0.07 0.07 1 42 2.4530 0.1173 L20.02 0.10 SL2 +/-0.05 0.05 +/--0.42 0.42 1 42 0.9881 0.3202 0.03 0.00 SL3 0.03

+/--0.10 +/--0.00 0.00 1 42 3.2267 0.0724

Micropogonias undulatus SL1 M5 0.01 42 2.6678 0.1024 0.04 0.01 SL2 0.09 0.04 1 42 1.9573 0.1618

+/- 0.43 0.03 0.25 SLU +/- 0.56 0.00 1 42 4.1341 0.0420

+/- 0.00 Orthopristischrysoptera SL3 1 42 1.9573 0.1618

+/- 0.09 - 0.04 SL2 <000.1142 0.0581 0.8095

+/- 0.01 +/- 0.04 SU 0.02 0.00 14 .65 OE

+/-0.08 +/-0.00 111 Pomatomrussaltatrix Ara Pr-prt Pos Upa 0.20 SL1 0.36 0.02 142 5.4332 0.0198

ý--0.08 SL2 000.1142 0.5982 0.4393

+/- 0.20 +0.03 0.10 SL3 +/- 0.28 0.00 1 42 4.1341 6.0420

+/- 0.00 Prionotusscitulus Ara P*ý u *irt Potur

< 0.01 0.01 SL2 < 0.02 0.03 1 42 0.0581 0.8095

+/- 0.01 +/- 0.04 SL+/- 0.01 0.0 1 42 1.0800 0.2987

+/- 0.09 3 0.03

SL +/- 0.06 0.48 +/-0.00 0.25 1 42 0.6445 0.4221 SL2 +/-40.02 0.27 1 0.24 0.16 1 42 0.3121 0.5764 0.00 0.001 S3 0.38 0.96 +/- 0.00 0.32 1 42 0.1667 0.6830 Trachinotuscarolinus 0.012 0.06 0.01 0.00 SLI ~0.00 0.006 2 0000 100 SL2 000.6142 0.4538 0.5006

--0.03 :E 0.24 SU0.01 0.00 14 3.2267 0.0724 SL I 4-0.03 0.0

+/-1.0 Umbrina coroides SL3 42 0.7500 0.3865

+/- 0.11 +/- 0.00 0.00 0.00 SL2 000.0142 0.0000 1.0000

+/-0.00 +/-0.00 SUL.03.0 42 0.0000 1.0000

+ 0.00 +/- 0.0011

Table 46. Mean Total Length (mm) of RIS Captured by Gill Netting, for All Events, by Pre- and Post-Uprate, Season, and Area, St.

Lucie Plant EPU.

SLU SL2 SL3 Winter Summer Winter Summer Winter Mm)

Taxa Summer JPost Pre Post Pre Post m Post Pre [ Post Pre I Post Pre Pre Scomberomorusmaculatus 480.0 446.5 510.9 493.3 415.0 483.4 475.6 502.0 485.7 476.4 Pomatomnussaltatrix 429.2 420.1 393.5 363.8 387.9 372.0 396.3 379.3 409.4 Menticirrhuslittoralis 378.0 377.0 377.7 Brevoortia smithi 337.6 340.6 334.2 343.6 367.5 359.6 333.3 341.6 Menticirrhusanericanus 361.7 352.0 332.3 376.8 314.3 239.5 344.0 332.3 Menticirrhussaxatilis 326.3 306.7 320.5 318.4 Micropogoniasundulatus 266.9 293.5 248.0 261.2 247.0 271.8 247.2 265.4 Cynoscion nothus 257.8 259.8 305.0 259.5 Trachinotus carolinus 270.0 242.9 250.7 277.0 250.1 Orthopristischrysoptera 233.4 240.7 237.0 201.0 256.0 250.0 247.7 241.1 Opisthonema oglinum 237.0 237.0 Leiostomnusxanthurus 236.1 208.8 207.0 233.8 258.0 219.4 Brevoortiatyrannus 218.3 218.3 Umbrina coroides 216.4 216.4 Prionotutsscitulus 184.0 174.0 156.0 190.0 223.6 190.0 203.3 Mean TL (mm) [323.8 205.5 1408.8 141.1.5 1310.0 1 NA 373.8 322.0 364.6 j502.0 309.1 467.2 344.0

Table 47. Mean Weight (g) of RIS Captured by Gill Netting for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SLU SL2 SL3 Mean Taxa Summer Winter Summer Winter Summer Winter Weight Pre ]Post Pre JPost Pre JPost Pre J Post Pre IPost Pre J Post (g)

Scomberomorus maculatus 677.8 471.8 682.2 787.4 380.0 572.7 709.2 775.0 645.0 670.7 Pomatomussaltatrix 776.5 702.4 480.0 400.0 555.7 490.0 618.1 515.0 666.3 Menticirrhus littoralis 435.0 545.0 471.7 Brevoortiassmithi 455.6 362.0 545.8 460.0 476.0 570.0 415.2 463.8 Menticirrhusaamericanus 505.0 445.0 356.7 585.0 322.1 315.0 441.3 400.9 Menticirrhussaxatilis 355.0 310.0 360.0 341.1 Micropogoniasundulatus 241.6 315.0 160.0 207.8 152.5 248.8 162.2 227.4 Trachinotuscarolinus 277.5 184.7 210.0 266.0 207.7 Orthopristischrvsoptera 201.9 216.7 120.0 120.0 220.0 246.7 213.7 207.5 Cynoscion nothus 181.5 162.6 200.0 175.4 Leiostomnus xanthurus 195.0 124.2 110.0 184.5 192.0 149.4 Umbrina coroides 147.1 147.1 Opisthonema oglinum 125.0 125.0 Prionotusscitulus 45.0 37.0 10.0 70.0 151.4 50.0 101.3 Brevoortia tyrannus 97.3 1 97.3 jMean Weight (g) 377.1 [81.0 534.3 1 493.8 33. 0.0 524.1 1325.7 1 485.8 1775.0 f348.7 ]607.8 1414.8

Table 48. Total Calculated Biomass (kg) of RIS Captured by Gill Netting for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SLI SL2 SL3 Total Taxa Summer Winter Summer Winter Summer Winter Biomass Pre ]Post Pre [Post Pre JPost Pre Post Pre J Post Pre Post (kg)

Scomberomorus inaculatus 25.1 16.0 11.6 32.3 0.4 6.3 70.9 1.6 9.7 173.0 Pomatomus saltatrix 24.1 17.6 1.0 1.6 3.9 1.0 16.7 1.5 67.3 Micropogonias undulatus 10.9 0.6 0.3 15.0 0.3 20.6 2.3 50.0 Brevoortiasmithi 5.0 1.8 3.3 2.3 1.0 9.7 17.9 41.3 Menticirrhusamericanus 5.1 0.9 1.1 2.3 8.4 0.6 5.7 24.1 Cynoscion nothus 11.1 5.4 0.4 16.8 Leiostomus xanthurus 2.0 9.2 0.2 2.0 0.2 14.6 Orthopristischrysoptera 1.6 0.7 0.1 0.1 0.2 0.7 2.4 5.8 Trachinotus carolinus 1.1 2.8 0.6 0.3 4.8 Menticirrhussaxatilis 1.4 0.9 0.7 3.1 Menticirrhuslittoralis 0.9 0.5 1.4 Prionotusscituius 0.1 0.04 0.01 0 1 1.1 0.1 1.3 Umbrina coroides 1.0 1.0 Brevoortia tyrannus 0.3 0.3 Opisthonenma oglinum 10.1 O._ 0.1 Total Biomass (kg) 78.1 0.2* 45.4 15.8' 8.1 ( 0.0 88.9 ]20.2 124.9 1 1.6 130.3 J 9.7 [ 418.6

Table 49. Total Calculated Biomass (g) From the Mean Weight of RIS Captured by Gill Netting for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

Area Total Season Pre- Post-Uprate SLU SL2 SL3 Biomass k(g)

Pre 78.1 81.1 124.9 285.6 Summer Post 0.2 0.0 1.6 1.7 Winter Pre 45.4 8.9 30.3 85.1 Winter Post 15.8 20.2 9.7 45.6 Total Biomass (kg) 138.7 110.9 T 166.5 418.6

Table 50. Total Number of Fish and Invertebrates Captured by Beach Seining for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SL1 SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total Pre [Post Pre [Post PreT[Post Pre [Post Pre [Post Pre [Post Crustacean Arenaeus cribrarius* 12 1 2 1 1 2 19 Albuneidae* 9 9 Emerita sp.* 6 1 8 Emerita talpoida* 5 2 7 Panulirusargus* 1 2 Libinia dubia 1 1 Crustacean Total [12 7J___ 11121 4 1 ___ 1 121 [ 1 46 Fish Harengulajaguana"* 5 27 6 1 116 1 18382 1188 19726 Umbrina coroides** 195 162 5 14 133 277 2 6 336 36 11 65 1242 Chloroscombrus chrysurus 960 96 1 9 18 118 7 1 1210 Sardinellaaurita** 155 185 701 4 1045 Eucinostomus gula 23 367 11 185 2 588 Anchoa lyolepis** 1 2 570 573 Menticirrhus littoralis** 68 81 2 61 65 60 5 5 142 39 31 2 561 Engraulis eurystole*

• 498 498 Caranx crvsos 199 1 2 68 270 Trachinotus carolinus** 59 8 1 42 15 3 78 17 1 2 226

SLI SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Selar crumenophthalmus 74 4 1 31 48 158 Harengulahumeralis** 1 138 15 154 Trachinotusfalcatus 6 11 5 15 3 5 13 4 2 1 10 75 Anchoa hepsetus*

• 14 59 73 Eucinostomus harengulus 28 15 1 44 Selene voiner 1 6 4 2 4 6 1 2 6 4 36 Eucinostomus argenteus 21 5 2 7 35 Elops saurus 1 I1 2 4 18 Opisthoneina oglinurn** 3 2 10 15 Centropornus undecimalis 1 2 4 2 1 4 14 Anchoa sp.** 1 1 11 13 Pomnatomnus saltatrix** 1 11 13 Caranx latus 1 1 2 7 11 Polydactylus virginicus 1 1 1 1 2 2 2 10 Eucinostomnusjonesii 5 2 7 Menticirrhusamericanus** 4 3 7 Bairdiellachrysoura 6 6 Menticirrhussaxatilis** 6 6 Mugil curema 1 6 Archosargusprobatocephalus 4 4 Micropogonias undulatus** 1 2 4 Carangoidesbartholomaei 3 3

SLI SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Caranxhippos 1 1 1 3 Polydactylus oligodon 2 1 3 Pseudocaranxdentex 3 3 Scomberomorus maculatus** 2 Selene setapinnis 1 1 2 Alosa sp.** I Bagre marinus 1 1 Caranx sp. 1 1 Citharichthysmacrops 1 Decapterusmacarellus 1 Diapterusauratus 1 1 Dorosoinapetenense*

• 1 Etrumeus teres*

• 1 Hemiramphus brasiliensis 1 Hyporhamphus unifasciatus 1 I Sphyrna tiburo 1 Trachinotus goodei 1 1 Fish Total 1812 407 45 95 1004 515 83 31 120575 1936 63 110 26676 Grand Total 1824 414 45 96 110061 519 84 31 20586 1937 69 111 26722

• Commercially or Recreationally Important Decapod Crustaceans

"*Representative Important Species

Table 51. Number of Fish and Invertebrates Captured Per Event by Beach Seining by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SLI SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total Pre Post Crustacean Arenaeus cribrarius* 2.40 0.33 0.40 0.33 0.33 0.40 1.36 Albuneidae* 1.80 0.64 Emerita sp.* 0.33 2.00 0.33 0.57 Emerita talpoida* 1.67 0.67 0.50 Panulirusargus* 0.33 0.33 0.14 Libiniadubia 0.33 0.07 Crustacean Total 2.40 2.33 0.00 0.33 0.40 1.33 0.33 0.00I 2.20 0.33 _2.00 [0.331 3.29 Fish Harengulajaguana** 1.00 9.00 2.00 0.20 38.67 0.33 3676.40 396.00 1409.00 Umbrina coroides** 39.00 54.00 1.67 4.67 26.60 92.33 0.67 2.00 67.20 12.00 3.67 21.67 88.71 Chloroscombruschrvsurus 192.00 32.00 0.33 3.00 3.60 23.60 2.33 0.33 86.43 Sardinellaaurita** 31.00 37.00 140.20 1.33 74.64 Eucinostomus gula 4.60 73.40 3.67 37.00 0.00 0.67 42.00 Anchoa iyolepis** 0.20 0.40 190.00 40.93 Menticirrhuslittoralis** 13.60 27.00 0.67 20.33 13.00 20.00 1.67 1.67 28.40 13.00 10.33 0.67 40.07 Engrauliseur stole *

• 99.60 35.57 Caranx crysos 39.80 0.33 0.40 13.60 19.29 Trachinotuscarolinus** 11.80 2.67 0.33 8.40 5.00 1.00 15.60 5.67 0.33 0.67 16.14 Selar crumenophthalmus 14.80 1.33 0.20 6.20 16.00 11.29

SL1 SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Harengulahumeralis** 0.20 27.60 3.00 11.00 Trachinotusfalcatus 1.20 3.67 1.67 3.00 1.00 1.67 4.33 0.80 0.67 0.33 3.33 5.36 Anchoa hepsetus* 4.67 19.67 5.21 Eucinostomus harengulus 5.60 3.00 0.20 3.14 Selene vomer 0.20 2.00 1.33 0.67 1.33 2.00 0.33 0.40 2.00 1.33 2.57 Eucinostomus argenteus 4.20 1.00 0.40 2.33 2.50 Elops saurus 0.33 3.67 0.67 1.33 1.29 Opisthonema oglinum** 1.00 0.40 3.33 1.07 Centropomus undecimalis 0.20 0.67 1.33 0.40 0.33 1.33 1.00 Anchoa sp.** 0.33 0.33 3.67 0.93 Pomatomus saltatrix** 0.20 0.20 3.67 0.93 Caranx latus 0.33 0.33 0.40 2.33 0.79 Polydactylus virginicus 0.20 0.33 0.33 0.33 0.40 0.67 0.67 0.71 Eucinostomusjonesii 1.00 0.40 0.50 Menticirrhus americanus** 1.33 1.00 0.50 Bairdiellachrysoura 2.00 0.43 Menticirrhussaxatilis*

• 1.20 0.43 Mugil curema 0.20 0.33 0.33 0.60 0.43 Archosargusprobatocephalus 0.80 0.29 Micropogoniasundulatus** 0.20 0.20 0.67 0.29 Carangoidesbartholomaei _ 1.00 0.21 Caranx hippos 0.33 0.33 0.20 0.21

SLi SL2 SL3 Taxa Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Polydactylus oligodon 0.67 0.33 0.21 Pseudocaranxdentex 1.00 0.21 Scomberomorus maculatus** 0.33 0.33 0.14 Selene setapinnis 0.20 0.33 0.14 Alosa sp. *_* 0.33 0.07 Bagre marinus 0.20 0.07 Caranx sp. 0.20 0.07 Citharichthysmacrops 0.20 0.07 Decapterusmacarellus 0.33 0.07 Diapterusauratus 0.33 0.07 Dorosomapetenense** 0.33 0.07 Etrumeus teres*

• 0.20 0.07 Heemiramphus brasiliensis 0.20 0.07 Iyvporhamphus unifasciatus 0.20 0.07 Sphyrna tiburo 0.33 0.07 Trachinotusgoodei I 1 1 0.33 1 1 1 0.07 Fish Total 362.40 135.67 15.00 31.67 200.80 171.67 27.67 10.33 4115.00 645.33 21.00 36.67 1905.43 Grand Total 364.80 138.00 15.00 32.00 201.20 173.00 28.00 10.33 14117.20 645.67 23.00 37.00 1908.71

• Commercially or Recreationally Important Decapod Cru

• Representative Important Species

Table 52. Mean Number of Fish Captured by Area by Beach Seining by Pre-and Post-Uprate, and Season, St. Lucie Plant EPU.

Pre- Post-r I Season P UprateT Pt SLI SL2 SL3 Total Pre 362.40 200.80 4115.00 4678.20 Summer Post 135.67 171.67 645.33 952.67 Pre 15.00 27.67 21.00 63.67 Winter Post 31.67 10.33 36.67 78.67 Total 168.50 116.64 1620.29 1905.43

Table 53. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Number of Taxa, Total Number of Individuals, and Biomass Among Areas for Fish Captured by Beach Seine During All Events, Seasons, Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Fish Taxa 3.10 2.95 3.60 All Events 2 126 0.5671 0.7531

-+/--

2.90 +/- 2.53 +/- 3.11 2.88 3.17 3.42 Pre-uprate 2 72 0.5511 0.7592

+/- 3.14 +/-2.87 +/-3.36 3.39 2.67 3.83 Post-uprate 2 54 1.0638 0.5875

+/- 2.59 +/- 8.61 +/- 2.81 Summerate 2 72 1.0865 0.5809

+/- 3.27

. 2.71 +/- 3.48 Winter 2 54 0.8152 0.6652 92.07 1.46 +/- 1.50 Total Fish Caught 56.17 38.88 540.10 All Events 2126 3.1357 0.2085

+/- 227.07 +/-_85.61 +/- 1689.75 Pre-uprate 773 52 5.2272 0.9622 0.6181 Sumerio +/- 297.99 +/--99.66 +/- 2185.81 2I 722 2.25U32 Post-uprate 278 03 1.7254 4.6935 0.0957

+/- 52.29 +/- 64.07 +291.97 27.789 92.46 60.33 63.29 91.61 937.96 Summer 272 2.3256 0.3126

+/- 297.47 +/-107.27  :*2168.95 Winter 2 54 3.3233 0.1898

+/- 17.20 +/-_12.10 +/- 10.52 _

Total Fish Caught Excluding Harengulajaguatia 55.26 36.07 74.14 All Events 2 126 3.0760 0.2148

+ 227.16 +/-84.03 +175.93 76.92 45.21 94.00 Pre-uprate 2 72 0.7228 0.6967

+/- 298.07 +/- 99.51 +219.36 26.39 23.89 47.67 Post-uprate 2 54 5.1733 0.0753

+/-_52.02 +/- 57.96 + 90.79 91.13 58.42 122.54 Summer 2 72 1.6855 0.4305

+297.72 +_106.13 +/-222.23 7.44 6.28 9.61 Winter 2 54 3.7373 0.1543

-_-17.23 +12.13 +/-10.52 RIS Fish Taxa All Events 2 126 2.5451 0.2801

+/- 1.38 + 1.53 +/- 1.52 _

1.33 2.00 2.13 Pre-uprate 2 72 2.9826 0.2251

+ 1.34 + 1.53 +/- 1.85 1.94 1.78 2.11 Post-uprate 2 54 0.7490 0.6876

+/- 1.39 + 1.56 +/- 0.96 1.96 2.67 2.71 Summer 2 72 3.3060 0.1915

+ 1.57 +/- 1.37 +/- 1.60 1.11 0.89 1.33 Winter 2 54 2.5514 0.2792

+/- 0.90 + 1.08 +/- 0.97

Total RIS Fish Caught All Events 207 71 2.82 126 5.1384 0.0766

+/- 49.69 + 56.93 +/- 1671.96 21.33 26.92 842.17 Pre-uprate 2 72 2.4518 0.2935

-52.27 + 54.09 + 2164.24 19.89 27.44 107.67 Post-uprate 2 54 5.4837 0.0645

+47.50 + 62.11 +/- 280.01 31.96 44.04 917.08 Summer 2 72 4.6639 0.0971

+/- 62.84 + 70.59 +2147.87 5.72 4.61 7.78 Winter 2 54 4.3465 0.1138

+/- 13.97 +/- 11.58 +/- 9.56 Total RIS Fish Caught Excluding Harengulajaguana 19.81 24.33 61.43 All Events 2 126 5.3871 0.0676

_ 49.54 +54.12 +/- 153.00 20.88 26.83 76.25, Pre-uprate 2 72 2.3181 0.3138

• 52.26 +_54.08 +/- 190.98 41.67 Post-uprate 18.39 21.00 + 79.91 2 54 5.9027 0.0523

• 47.11 +55.55 30.63 39.17 101.67 Summer 2 72 3.9845 0.1364

+/- 62.81 +/- 67.72 +_194.22 5.39 4.56 7.78 Winter 2 54 4.8876 0.0868

+/- 14.03 +/- 11.60 +/- 9.56

RIS Fish Biomass 407.47 180.43 6500.40 All Events 2 126 5.3838 0.0678

+/- 1260.99 + 328.02 + 19847.97 590.18 192.05 10727.15 Pre-uprate 2 72 1.9535 0.3765

+/- 1629.70 +/- 340.73 25579.24 163.85 164.93 864.72 Post-uprate 2 54 4.4273 0.1093

+/- 362.88 +/- 319.35 +/-2453.22 638.92 302.34 11233.18 Summer 2 72 3.7701 0.1518

+/- 1634.27 + 392.89 + 25448.81 98.87 190.01 Winter +212.16 17.87 + 296.52 2 54 7.1885 0.0275

+/-41.12 RIS Fish Biomass Excluding Harengulajaguana 402.07 170.92 1324.91 All Events 2 126 5.7278 0.0570

+/- 1287.67 - 325.84 - 5038.14 584.31 190.79 2119.81 Pre-uprate 2 2 2.0045 0.3671

• 1665.70 +/- 340.06 +/- 6604.32 Post-uprate 54 4.9547 0.0840

+/-368.38 +/-313.53 +/-353.09 643.09 286.30 2176.09 Summer 72 3.0924 0.2131

+/-1668.26 +/-394.45 6589.28 80.71 190.01 Winter +/-205.3 7 17.09 +/- 296.52 2 54 8.5446 0.0140

+/-41.33 _ 1- I _I

Table 54. Total Number of RIS Specimens Captured by Beach Seining for All Events, by Pre- and Post-Uprate, Season, and Area, St.

Lucie Plant EPU.

SL1 SL 2 SL 3 Taxa Harengulajaguana Summer 5

PostPre Po 27 Pre 6

Winter IPost Summer Pre I Post 1 116 Pre 1

J Winter Post Summer Pre 18382

__Post 1188 Pre Winter Post Total 19726 Umbrina coroides 195 162 5 14 133 277 2 6 336 36 11 65 1242 Sardinella aurita 155 185 701 4 1045 Anchoa lyolepiss 2 570 573 Menticirrhuslittoralis 68 81 2 61 65 60 5 5 142 39 31 2 561 Engraulis ewystole 498 498 Trachinotus carolinus 59 8 1 42 15 3 78 17 1 2 226 Harengulahumeralis 1 138 15 154 Anchoa hepsetus 14 59 73 Opisthoneniaoglinum 3 2 10 15 Anchoa sp. 1 1 11 13 Pomatomus saltatrix 1 1 11 13 Menticirrhusarnericanus 4 3 7 Menticirrhussaxatilis 6 6 Micropogoniasundulatus 1 1 2 4 Scomberomorus maculatus 1 1 2 Alosa sp. 1 1 Dorosomapetenense 1 1 Etrumneus teres 1 1 1Total 48 8 8 7 7 8 1 12 216 15 6 8 46

Table 55. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Number of Taxa, Total Number of Individuals, and Biomass Among Pre- and Post-Uprate Events Within Each Area for Fish Captured by Beach Seine, St. Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Fish Taxa SLI 2.88 3.39 1 42 0.9798 0.3223

+/- 3.14 +/- 2.59 3.17 2.67 SL2 1 42 0.0280 0.8670

+2.87 S +/- 2.03 3.42 3.83 SL3 I 42 1.0288 0.3104

+/- 3.36 +/- 2.81 Total Fish Caught 77.38 27.89 SLI 1 42 0.5886 0.4429

+/- 297.99 +/- 52.29 _

45.29 30.33 SL2 1 42 0.3302 0.5655

+/- 99.66 +/- 64.07 859.92 113.67 SL3 1 42 1.1174 0.2905

+/-2185.81 +/-291.97 Total Fish Caught Excluding Harengulajaguana

'Ma Vau PrAea+/-S

RIS Fish Taxa SL1.94 1 42 2.0436 0.1528

+/- 1.34 + 1.39 2.00 1.78 SL2 1 42 0.2309 0.6308

+ 1.53 +/- 1.56 2.13 2.11 SL3 1 42 0.0969 0.7556

+1.85 +0.96 Total RIS Fish Caught 21.33 19.89 SLI 1 42 1.4979 0.2210 L+52.27 +/- 47.50 26.92 27.44 SL2 1 42 0.3646 0.5459

+/- 54.09 +/- 62.11 842.17 107.67 SL3 1 42 0.6456 0.4217

+/- 2164.24 +/- 280.01 Total RIS Fish Caught Excluding Harengulajaguana 20.88 18.38 SLI 1 42 1.1879 0.2758

+/- 52.26 +/-47.11 26.83 21.00 SL2 1 42 0.4658 0.4949

+/- 54.08 +/- 55.55 76.25 41.67 SL3 1 42 1.4681 0.2256

+/- 190.98 +/- 79.91 _ I

d nIHIIII RIS Fish Biomass SL590.18 163.85 1 42 0.8334 0.3613

-- 1629.70 +/- 362.88 192.05 164.93 SL2 1 42 0.0533 0.8175

-340.73 +/- 319.35 10727.15 864.72 SL3 1 42 1.0909 0.2963

+/-_25579.24 +/- 2453.22 ,

RIS Fish Biomass Excluding Harengulajaguana 584.31 159.08 SLI 142 0.8693 0.3512

.4-1665.70 +_368.38 _________

190.79 144.44 SL2 142 0.0952 0.7576

+/--340.06 ++/-313.53 ____ ________

2119.81 265.05 SL3 142 1.5581 0.2119

________ +/-6604.32

- +/-- 353.09 _____ _________ ____

Table 56. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Total Individuals Among Areas for RIS Fish Captured by Beach Seine During All Events, Seasons, Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Clupeiformes 4.17 9.43 42.90 All Events 2 126 1.8890 0.3889

+23.94 +/-32.09 + 130.19 7.08 16.04 50.71 Pre-uprate 2 72 2.5748 0.2760

+ 31.64 +/-41.54 + 158.80 0.28 0.61 32.50 Post-uprate 2 54 1.2010 0.5485

+/- 0.57 +/- 2.35 +/- 81.01 6.71 14.00 74.63 Summer 2 72 3.5276 0.1714

+/-31.59 +/-41.26 +/-_166.61 Winter 0.78 3.33 0.61 2 54 1.8230 0.4019

+/- 3.30 -- 10.59 +/- 2.59 0.4019 Menticirrhus Spp.

+/-11.14 +/-6.17 +/-13.29 2.92 3.17 7.21 Pre-uprate 2 72 2.8486 0.2407

+/- 6.51 +/- 6.08 + 17.14 7.89 3.83 2.44 Post-uprate 2 54 1.2089 0.5464

• 15.06 +/- 6.45 +/- 3.87 6.21 5.63 7.54 Summer 2 72 0.2931 0.8637

+/- 10.22 +/-7.44 +/-16.73 3.50 0.56 2.00 Winter 2 54 0.7930 0.6727

______- +/-12.39 +/--1.15 +/- 5.39 ____ ____________

Micropogoniasundulatus All Events 2 126 0.5123 0.7740

+/- 0.15 +/- 0.15 +/- 0.22 0.04 0.04 0.08 Pre-uprate 2 72 0.5221 0.7703 1+/-0.20 +/- 0.20 +/- 0.28 0.00 0.00 0.00 Post-uprate 2 54 0.0000 1.0000

+ 0.00 +/- 0.00 +/- 0.00 0.04 0.04 0.00 Summer 2 72 1.0143 0.6022

+/- 0.20 +_0.20 +_0.00 0.00 0.00 0.11 Winter 2 54 4.0769 0.1302

+ 0.00 +/- 0.00 +/- 0.32 Pomatomus saltatrix 0.00 0.02 0.29 All Events 2 126 2.0485 0.3591

+/- 0.00 +/- 0.15 +/- 1.70 0.00 0.04 0.50 Pre-uprate 2 72 2.0861 0.3524

+/- 0.00 +/- 0.20 +/- 2.25 0.00 0.00 0.00 Post-uprate 2 54 0.0000 1.0000

________ +/-_0.00 +/-_0.00 +/-_0.00 ____

0.00 0.04 0.04 Summer 2 72 1.0143 0.6022

+/- 0.00 +/-_0.20 +/-_0.20 0.00 0.00 0.61 Winter 2 54 2.0000 0.3679

________ +/-_0.00 +/-_0.00 +/-_2.59 _________

Scomberomorus maculatus All Events 0.00 0.02 0.02 2 126 1.0081 0.6041

+/- 0.00 + 0.15 +/- 0.15 _

0.00 0.04 0.00 Pre-uprate 2 72 2.0000 0.3679

+/- 0.00 + 0.20 +/- 0.00 0.00 0.00 0.06 Post-uprate 2 54 2.0000 0.3679

+/- 0.00 +/- 0.00 +/- 0.24 0.00 0.00 0.00 Summer 2 72 0.0000 1.0000

+/- 0.00 +/-_0.00 +/- 0.00 0.00 0.06 0.06 Winter 2 54 1.0192 0.6007

+/-_0.00 +_0.24 +/- 0.24 i Trachinotus carolinus 1.62 1.43 2.33 All Events 2 126u 3.6593, 0.1605.

________ +/-_6.40 +/--_2.60 +/-_5.61 ____________________

2.50 1.88 3.29 Pre-uprate 2 72 1.9860 0.3705

+/- 8.39 +/- 3.18 + 7.18 ________ ___ ____

+ 0.44 +/- 0.83 +- 1.06 Post-uprate 2 54 1.9379 0.3795

+/- 1.10 +-1.38 +/- 1.80 ___ ___ ________

2.79 2.38 3.96 Summer 2 72 3.2481 0.1971

+-_8.35 +/--_3.10 +/-_7.04 ________ ____ ____

0.06 0.17 0.17 Winter 2 54 0.48 10 0.7862

_______ +0.24

- +/-_0.51 +/-_0.51 ____ ________ ____

Umbrina coroides 8.95 9.95 10.67 All Events 2 126 2.6605 0.2644

+ 28.07 +/- 34.34 +/- 34.63 8.33 5.63 14.46 Pre-uprate 2 72 0.6595 0.7191

+/- 20.74 +/- 13.77 +/- 45.49 9.78 15.72 5.61 Post-uprate 2 54 2.9919 0.2240

_______ +36.28

+/- +/-_50.26 +/-_6.77 ____

14.88 17.08 15.50 Summer 2 72 1.1415 0.5651

• 36.29 +_44.47 +_42.25 4.22 Winter 1.06 0.44 + 6.74 2 54 6.6897 0.0353

+ 1.66 +/-1.20 1 1 1

Table 57. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Total Individuals Among Pre- and Post-Uprate Events Within Each Area for RIS Fish Captured by Beach Seine, St. Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Clupeiformes 7.08 0.28 SLI 1 42 0.4703 0.4928

• 31.64 +/- 0.57 16.04 0.61 SL2 1 42 2.3734 0.1234

+ 41.54 + 2.35 50.71 32.50 SL3 1 42 0.1307 0.7177

+158.79 +81.01 Mentieirrhus Spp.

2.92 7.89 SLI 1 42 3.5183 0.0607

+/- 6.51 +15.06 SL2 1 42 0.2267 0.6340

+6.08 -+6.45 SL3 1 42 0.8137 0.3670

+ 17.14 +/- 3.87 Micropogoniasundulatus 0.04 0.00 SLI 1 42 0.7500 0.3865

+ 0.20 + 0.00 0.04 0.00 SL2 1 42 0.7500 0.3865

+ 0.20 +/- 0.00 0.08 0.00 SL3 1 42 1.5375 0.2150

+/- 0.28 +/- 0.00

Pornatomussaltatrix

[ 0.00 0.00 SLI 01 42 0.0000 1.0000

+/-10.00 +/- 0.00 0.04 0.00 SL2 1 42 0.7500 0.3865

+/- 0.20 +/- 0.00 0.50 0.00 SL3 1 42 1.5366 0.2151

+2.25 4-0.00 Scomberomortis maeulatus 0.00 0.00 SLI 1 42 0.0000 1.0000

+/-: 0.00 +/- 0.00 0.04 0.00 SL2 1 42 0.7500 0.3865

+/- 0.20 +/--0.00 0.00 0.06 SL3 42 1.3333 0.2482 A-0.00 A-0.24 Trachinotus carolinus 2.50 0.44 SLI 1 42 0.9413 0.3319

+8.39 +/- 1.10 1.88 0.83 SL2 42 1.2835 0.2572

+/-3.18 +/- 1.38 3.29 1.06 SL3 42 0.2254 0.6350

+/- 7.18 +/- 1.80

Umbrina coroides 8.33 9.78 SLI 1 42 1.0138 0.3140

• 20.74 +/- 36.28 5.63 15.72 SL2 1 42 0.6022 0.4377

• 13.77 + 50.26 14.46 5.61 SL3 1 42 1.9797 0.1594

• 45.49 + 6.77

Table 58. Mean Total Length (mm) of RIS Captured by Beach Seining, for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SLI SL2 SL3 Summer Winter Summer Winter Summer Winter Mean TL Taxa Pre [ Post Pre I Post Pre I Post Pre__ Post Pre J Post Pre Post (mm)

Scomberomorus maculatus 28.00 374.00 201.00 Micropogoniasundulatus 240.00 220.00 168.50 199.25 Menticirrhuslittoralis 82.70 96.91 241.50 96.89 90.97 79.73 85.84 156.02 165.85 88.52 78.32 137.50 103.91 Dorosomapetenense 102.30 102.30 Trachinotuscarolinus 101.57 97.06 69.00 91.72 82.68 50.67 94.69 69.72 390.00 180.50 94.19 Umnbrinacoroides 125.15 75.54 74.48 65.29 82.70 74.28 54.10 56.65 101.73 70.14 86.22 106.38 93.86 Harengulajaguana 68.00 80.90 167.67 107.00 70.24 117.00 86.08 85.53 85.01 Sardinellaaurita 104.08 68.24 79.75 122.20 83.43 Engrauliseurystole 73.80 73.80 Harengulahumeralis 69.90 73.13 69.48 72.33 Opisthonernaoglinum 68.57 92.50 66.60 70.45 Anchoa lyolepis 60.30 40.85 69.92 69.19 Menticirrhusamericanus 63.70 71.00 66.83 Pomatomus saltatrix 49.20 132.00 43.90 51.68 Menticirrhussaxatilis 51.17 51.17 Etrurneus teres 38.00 38.00 Anchoa hepsetus 29.20 32.17 31.70 Anchoa sp. 18.10 28.00 1 26.01 25.55 Mean TL (mm) 1106.46187.71 190.06 1 79.20 1 82.54 J 75.31 138.48 [95.67 1102.861 78.46 182.45 1100.29[ 88.92

Table 59. Mean Weight (g) and Total Biomass (g) for RIS Captured by Beach Seine, for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SLU SL2 SL3 Summer Winter Summer Winter Summer Winter

-- Total Taxa Pre- Post- Pre- Post- Pre- Post- Pre- Post- Pre- Post- Pre- Post- Biomass' Uprate Uprate Uprate Uprate Uprate Uprate Uprate Uprate Uprate Uprate Uprate Uprate (g)

Mean Mean Mean Mean Mean Mean Mean Mean Mean Mean Mean Mean Wt (g) Wt (g) Wt (g) Wt (g) Wt (g) Wt (g) Wt (g) Wt (g) Wt (g) Wt (g) Wt (g) Wt (g)

Harengulajaguana 15.2 113.2 327.0 15.0 407.4 14.0 134418.4 10375.6 160126.8 Umbrina coroides 11204.6 898.4 44.8 48.9 1083.1 2163.3 3.1 14.0 4169.8 159.7 223.3 1676.7 25291.0 Menticirrhuslittoralis 729.7 937.4 796.0 819.6 864.3 399.8 31.8 245.1 12352.3 312.1 212.1 53.7 15133.4 Sardinella aurita 1891.0 666.0 2124.3 60.0 5456.0 Trachinotuscarolinus 1080.4 154.6 5.1 724.4 193.7 5.7 1485.0 108.9 708.0 160.4 4663.3 Eng-raulis eurstole 1593.6 1593.6 Anchoa lyolepis 1.4 0.8 1235.6 1215.6 Micropogonias undulatus 380.0 119.4 117.0 616.4 Harengulahumeralis 3.5 563.6 48.6 600.6 Scomberomorus maculatus 0.1 244.0 244.1 Opisthoneinaoglinumn 9.5 13.3 26.3 49.1 Pomnatomnus saltatrix 1.0 27.0 8.4 38.6 Menticirrhusamnericanus 10.3 15.7 26.0 Menticirrhussaxatilis 16.2 16.2 Dorosomapetenense 9.2 9.2 Anchoa hepsetus 1.1 7.8 9.1 Anchoa sp. 0.1 0.1 0.9 1.1 Alosa sp. 0.5 0.5 Etruneus teres 0.3 0.3 Total Biomass (g) 15304.4 2122.4 [ 1174.0 J 868.5 4067.6 3200.8 1 62.5 1 259.2 1156220.11 12252.5 1268.8 1 2151.4 1215090.9 Total Biomass calculated by multiplying the number collected by the mean weight for specimens weighed.

Table 60. Total Calculated Biomass (kg) of RIS Captured by Beach Seining for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

Area Pre- Post- I T Total Uprate Bi ios SL3 Biomass Biomass (kg)

Uprte Biomass__ Biomass Pre 20.0 3.6 505.4 516.1 Summer Post 2.2 2.2 10.2 14.9 Pre 0.5 0.0 0.3 0.4 Winter Post 0.5 0.2 1.8 3.5 Total Biomass (kg) 25.4 5.2 382.0 388.3

Table 61. Ichthyoplankton and Commercially or Recreationaly Important Decapod Crustaceans Captured by Plankton Netting for All Events, by Pre-and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

SLI SL2 SL3 Taxa Life Stage Summer Winter Summer Winter Summer Winter Total Pre Post Poe Pre Post Post Pre Post jPre Post Crustacean Callinectes sp. Megalops 173 9 507 3 95 6 196 1 25 20 197 32 1264 Albunea sp. Zoea 36 103 9 20 152 330 206 9 89 160 11 82 1207 Callinectes sapidus Megalops 156 325 132 134 190 116 1053 Menippe mercenaria Zoea 15 60 120 6 240 27 262 3 83 56 11 139 1022 Callinectes sp. Zoea 70 13 112 63 54 6 358 30 47 3 88 108 952 Emerita talpoida Zoea 27 124 12 30 91 83 40 17 81 43 34 193 775 Callinectes simnilis Megalops 11 16 112 246 385 Penaeidae Post Larvae 24 13 2 180 219 Farfantepenaeussp. Post Larvae 2 3 26 6 3 1 26 29 48 1 34 179 Penaeidae Mysis 1 1 5 10 1 28 17 9 11 66 20 169 Penaeidae Protozoea 4 14 12 61 15 37 3 5 7 8 166 Fatfantepenaeus sp. Mysis 9 151 1 161 Faifantepenaeusaztecus Post Larvae 27 28 4 30 1 4 10 15 9 128 Faifantepenaeusduoraruin Post Larvae 22 1 14 4 15 33 8 14 111 Penaeidae Other 14 11 33 58 Portunidae Zoea 7 13 7 1 4 13 45 Lepidopa websteri Zoea 13 26 39 Portunidae Megalops 6 20 8 1 35 Scyllarus americanus Phyllosoma 2 30 2 1 35 Menippe mercenaria Megalops 16 1 1 1 2 7 3 31 Lepidopa sp. Zoea 3 2 8 4 7 3 27 Rimapenaeus constrictus Mysis 1 24 1 26 Rirnapenaeusconstrictus Post Larvae 8 2 1 6 1 18

SLI SL2 SL3 Taxa Life Stage Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Farfantepenaeussp. Other 14 14 Emerila talpoida Juvenile 3 4 12 Emerita talpoida Megalops 8 1 9 Callinectessimnilis Juvenile 2 6 8 Callinectes sp. Juvenile 1 2 3 Sicyoniidae Mysis 1 2 3 Sicyoniidae Post Larvae 1 3 Callinectes sapidus Juvenile 2 2 Hippidae Zoea 2 2 Menippe sp. Zoea 2 2 Sicvonia sp. Mysis 2 2 Sicyonia sp. Post Larvae 1 1 2 Callinectes sp. Post Larvae 1 Hippoidea Zoea 1 Menippe sp. Megalops 1 1 Scyllaridae Phyllosoma I 1 Sicyonia brevirostris Mysis I Sicyonia sp. Juvenile 1 Squillidae Pseudozoea 1 Unidentified shrimp Post Larvae 1 Xiphopenaeus kroveri Mysis I1 Crustacean Total 603 361 .822 527 11234 f508 11431 308 I901 577 414 778 8176 Fish Unidentified eggs Egg 527 5588 2895 740 666 7490 2329 466 6245 2454 2404 1473 33277 Clupeidae Egg- __ 120 386 61 6764 724 136 1 27 1327 26 9572 Clupeidae Post Yolk-Sac Larvae 65 24 925 245 183 202 80 219 32 110 16 10 2111

SLI SL2 SL3 Taxa Life Stage Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Clupeiformes Egg 1012 1012 Microgobius thalassints Post Yolk-Sac Larvae 126 1 1 417 2 17 5 1 1 571 Synodontidae Egg 3 143 23 5 114 86 374 Gobiidae Post Yolk-Sac Larvae 35 4 107 8 23 29 88 15 6 12 15 8 350 Sciaenidae Post Yolk-Sac Larvae 5 43 2 31 19 25 22 27 9 15 93 23 314 Engraulidae Egg 88 17 87 1 193 Unidentified fragment Post Yolk-Sac Larvae 5 1 27 7 19 20 47 3 9 28 166 Carangidae/ Labridae/ Egg 163 163 Sciaenidae egi complex Sciaenidae Egg 2 27 6 7 31 28 43 144 Engraulidae Post Yolk-Sac Larvae 10 44 47 12 2 15 1 10 141 Microgobiusgulosus Post Yolk-Sac Larvae 3 3 44 32 6 5 28 121 Blenniidae Post Yolk-Sac Larvae 1 9 1 4 5 5 11 5 27 18 17 103 Gobiosoinarobustum Post Yolk-Sac Larvae 3 7 1 30 8 34 6 3 4 3 99 Etrumeus leres Post Yolk-Sac Larvae 41 45 86 Anchoa sp. Post Yolk-Sac Larvae 10 2 18 26 56 Haemulidae Post Yolk-Sac Larvae 1 5 4 2 1 37 2 1 1 54 Clupeidae Yolk-Sac Larvae 25 18 43 Brevoortiasmithi Post Yolk-Sac Larvae 11 2 28 41 Cynoscion sp. Post Yolk-Sac Larvae 3 3 4 11 4 8 4 37 Microdesmidae Post Yolk-Sac Larvae 29 1 5 35 Perciformes Post Yolk-Sac Larvae 21 2 1 2 1 1 3 2 33 Sparidae Egg 32 32 Anchoa mitchilli Post Yolk-Sac Larvae 24 1 5 30 Eucinostomus sp. Post Yolk-Sac Larvae 8 3 3 1 1 1 12 29 Carangidae Post Yolk-Sac Larvae 9 1 7 1 3 1 1 23 Diodontidae Post Yolk-Sac Larvae 1 16 2 2 1 23

SL1 SL2 SL3 Taxa Life Stage Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Unidentified fish - damaged Post Yolk-Sac Larvae 1 1 3 8 4 6 23 Larimusfascialus Post Yolk-Sac Larvae 1 2 19 22 Clenogobius boleosoma Post Yolk-Sac Larvae 1 5 1 4 10 21 Gobiosoma sp. Post Yolk-Sac Larvae 5 9 6 1 21 Sparidae Post Yolk-Sac Larvae 1 4 6 1 4 3 1 20 Clupeiformes Post Yolk-Sac Larvae 2 4 10 16 Dactyloscopidae Post Yolk-Sac Larvae 8 4 1 3 16 Achirus linealtus Post Yolk-Sac Larvae 2 1 7 2 2 1 15 Sciaenidae Yolk-Sac Larvae 6 1 8 15 Bathygobius soporator Post Yolk-Sac Larvae 2 2 3 1 1 1 4 14 Bregmacerotidae Post Yolk-Sac Larvae 2 3 1 1 6 13 Labridae Post Yolk-Sac Larvae 1 2 1 3 2 2 2 13 Tetraodontidae Post Yolk-Sac Larvae 4 1 2 1 5 13 Chloroscombruschtysurus Post Yolk-Sac Larvae 1 11 12 Lutjanidae Post Yolk-Sac Larvae 2 1 1 6 2 12 Lutjanus sp. Post Yolk-Sac Larvae 11 1 12 Monacanthidae Post Yolk-Sac Larvae 2 5 4 1 12 Anchoa lyolepis Post Yolk-Sac Larvae 11 11 Cynoscion nebulosus Post Yolk-Sac Larvae 3 3 2 1 2 11 Gerreidae Post Yolk-Sac Larvae 8 1 1 1 11 Myctophidae Post Yolk-Sac Larvae 5 2 1 1 2 11 Paralichthyidae Post Yolk-Sac Larvae 1 2 2 4 2 11 Cerdalefloridana Post Yolk-Sac Larvae 10 10 Citharichthysspilopterus Post Yolk-Sac Larvae 1 6 3 10 Micropogoniasundulatus Post Yolk-Sac Larvae 2 7 1 10 Pareques sp. Post Yolk-Sac Larvae 1 2 4 3 10

SLI SL2 SL3 Taxa Life Stage Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Cynoscion regalis Post Yolk-Sac Larvae 5 2 2 9 Unidentified fish Post Yolk-Sac Larvae 7 1 9 Bairdiellachlysoura Post Yolk-Sac Larvae 1 3 1 8 Sciaenops ocellatus Post Yolk-Sac Larvae 4 1 2 1 8 Serranidae Post Yolk-Sac Larvae 3 1 31 8 Sparisomia sp. Post Yolk-Sac Larvae 2 1 2 2 8 Stomiiformes Post Yolk-Sac Larvae 4 2 2 8 Eleotridae Post Yolk-Sac Larvae 1 6 7 Gobiesox strunosus Post Yolk-Sac Larvae 2 2 2 1 7 Gonostomatidae Post Yolk-Sac Larvae 1 1 41 7 Harengulajaguana Post Yolk-Sac Larvae 7 7 Ophidiiformes Post Yolk-Sac Larvae 5 1 7 Ostraciidae Post Yolk-Sac Larvae 4 2 1 7 Anguilloidei Post Yolk-Sac Larvae 6 6 Apogonidae Post Yolk-Sac Larvae 2 4 6 Prionotus sp. Post Yolk-Sac Larvae 1 4 6 Brevoortia sp. Post Yolk-Sac Larvae 2 3 5 Menticirrhus amnericanus Post Yolk-Sac Larvae 4 1 5 Micropogoniasfiirnieri Post Yolk-Sac Larvae 5 5 Monacanthus ciliatus Post Yolk-Sac Larvae 2 2 5 Ophidion sp. Post Yolk-Sac Larvae 3 2 5 Parablenniusmarmoreus Post Yolk-Sac Larvae 4 15 Parequesacuminatus Post Yolk-Sac Larvae 1 4 5 Scaridae Post Yolk-Sac Larvae 2 1 5 Selene selapinnis Post Yolk-Sac Larvae 5 5 Stellifer lanceolatus Post Yolk-Sac Larvae 5 5

SLI SL2 SL3 Taxa Life Stage Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Syinphurus sp. Post Yolk-Sac Larvae 2 2 1 5 Syngnathus louisianae Juvenile 1 4 5 Diplospinusmullistriatus Post Yolk-Sac Larvae 4 4 Pomacentridae Post Yolk-Sac Larvae 1 2 1 4 Sauridabrasiliensis Post Yolk-Sac Larvae 1 3 4 Scarms sp. Post Yolk-Sac Larvae 4 4 Selar crumenophthalmus Post Yolk-Sac Larvae 4 4 Sphyraenidae Post Yolk-Sac Larvae 4 4 Tetraodontidae Yolk-Sac Larvae 4 4 Balistidae Post Yolk-Sac Larvae 3 3 Chaelodipterusfaber Post Yolk-Sac Larvae 2 1 3 Labrisomidae Post Yolk-Sac Larvae 1 1 3 Menticirrhus sp. Post Yolk-Sac Larvae 21 3 Ophidion sp. Yolk-Sac Larvae 3 3 Pleuronectiformes Post Yolk-Sac Larvae 3 3 Scorpaenidae Post Yolk-Sac Larvae 2 1 3 Sphoeroides sp. Post Yolk-Sac Larvae 2 1 3 A/uterus schoepfii Post Yolk-Sac Larvae 1 1 2 Atheriniformes Post Larvae 2 2 Brevoortia tvrannus Post Yolk-Sac Larvae 2 2 Chasmodes saburrae Post Yolk-Sac Larvae 1 1 2 Clupeiformes Yolk-Sac Larvae 2 2 Diplectruin sp. Post Yolk-Sac Larvae 2 2 Elops saurus Post Yolk-Sac Larvae 1 2 Epinephelus sp. Post Yolk-Sac Larvae 2 2 Gobiosoma bosc Post Yolk-Sac Larvae 2 2

SLU SL2 SL3 Taxa Life Stage Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Hygophum reinhardtii Post Yolk-Sac Larvae 2 2 Leiostomnus xanthurus Post Yolk-Sac Larvae 1 1 2 Microdesmus sp. Post Yolk-Sac Larvae 2 2 Mullidae Post Yolk-Sac Larvae 2 2 Opistognathidae Post Yolk-Sac Larvae 2 2 Percophidae Post Yolk-Sac Larvae 2 2 Triglidae Post Yolk-Sac Larvae 2 Acanthurus sp. Post Yolk-Sac Larvae 1 Achiridae Egg 1 1 Acropomatidae Post Yolk-Sac Larvae 1 Astrapogon puncticulatus Post Yolk-Sac Larvae 1 Atheriniformes Post Yolk-Sac Larvae 1 Atherinopsidae Post Yolk-Sac Larvae 1 1 Bothidae Post Yolk-Sac Larvae 1 1 Bramidae Post Yolk-Sac Larvae 1 Citharichthysarctifrons Post Yolk-Sac Larvae 1 Citharichthys sp. Juvenile 1 Congridae Post Yolk-Sac Larvae 1 1 Coryphaena hippurus Post Yolk-Sac Larvae I Clenogobius sp. Post Yolk-Sac Larvae 1 Cyclothone sp. Post Yolk-Sac Larvae 1 1 Diodon sp. Post Yolk-Sac Larvae 1 1 Diogenichthys atlanticus Post Yolk-Sac Larvae 1 Dormitatormaculatus Post Yolk-Sac Larvae 1 Eleotrispisonis Post Yolk-Sac Larvae 1 Engraulidae Yolk-Sac Larvae 1

SLI SL2 SL3 Taxa Life Stage Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Euthynnus alletteratus Post Yolk-Sac Larvae 1 1 Gempylidae Post Yolk-Sac Larvae 1 1 Gobionelhls oceanicus Post Yolk-Sac Larvae 1 Gobiosoniaparri Post Yolk-Sac Larvae 1 1 Haemiulon sp. Post Yolk-Sac Larvae 1 1 Hippocampus erectus Post Yolk-Sac Larvae 1 Lupinoblennius nicholsi Post Yolk-Sac Larvae 1 Melarnphaidae Post Yolk-Sac Larvae 1 Melanocetidae Post Yolk-Sac Larvae 1 Merlucciidae Post Yolk-Sac Larvae 1 AMugil cephalus Post Yolk-Sac Larvae I Nes longus Post Yolk-Sac Larvae 1 Ogcocephalus sp. Post Yolk-Sac Larvae 1 Ophidiidae Post Yolk-Sac Larvae 1 Paralepididae Post Yolk-Sac Larvae 1 Phosichthyidae Post Yolk-Sac Larvae 1 Pomacanthidae Post Yolk-Sac Larvae 1 Scomberomorus maculatus Post Yolk-Sac Larvae 1 Scorpaeniformes Post Yolk-Sac Larvae 1 1 Stephanoberyciformes Post Yolk-Sac Larvae 1 1 Syngnathidae Post Yolk-Sac Larvae 1

.Syngnathus louisianae Post Yolk-Sac Larvae 1 Syngnathus sp. Post Yolk-Sac Larvae 1 Tetraodontiformes Egg 1 1 Trichiuruslepturus Post Yolk-Sac Larvae 1 Umbrina coroides Post Yolk-Sac Larvae 1

SLI SL2 SL3 Taxa Life Stage Summer Winter Summer Winter Summer Winter Total Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Unidentified fragment Yolk-Sac Larvae 1 1 Uranoscopidae Post Yolk-Sac Larvae I I I 1 Fish Total 1132 15919 5615 1170 1807 14654 [3427 J1062 J6516 12750 14121 [ 1734 [ 49907 Total 1735 ]6280 6437 J1697 3041 115162 (4570 1 1370 J7417 J3327 ]4535 1 2512 [58083

Table 62. Total Number of Larval Fish Captured in Plankton Tows for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

Season Pre- Post- SL3 Total Uprate Pre 351 904 127 1382 Summer Post 211 400 269 880 Winter Pre 1260 320 261 2176 Post 346 455 234 1035 Total 2168 2079 891 5473

Table 63. CPUE (Number Collected Per 100m3 of Water Filtered) for Fish and CRI Decapod Crustacean Larvae Captured in Plankton Tows, for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

Taxa 1ý

" U I..,~~1 S)La%.eanIs .

Callinectes sp. Megalops 3.1784 0.1878 7.4015 0.0760 1.5055 0.1041 3.9365 0.0258 0.3470 0.3686 4.4864 0.5302 1.9443 Albunea sp. Zoea 0.6614 2.1490 0.1314 0.5068 2.4089 5.7272 4.1374 0.2326 1.2354 2.9488 0.2505 1.3585 1.8567 Callinectessapidus Megalops 2.8661 8.2362 2.0919 3.4634 2.6374 1.9218 1.6198 AIfenippemercenaria Zoea 0.2756 1.2518 1.7518 0.1521 3.8035 0.4686 5.2621 0.0775 1.1521 1.0321 0.2505 2.3028 1.5721 Callinectessp. Zoea 1.2861 0.2712 1.6350 1.5966 0.8558 0.1041 7.1902 0.7754 0.6524 0.0553 2.0041 1.7893 1.4644 Emnerita talpoida Zoea 0.4960 2.5871 0.1752 0.7603 1.4422 1.4405 0.8034 0.4394 1.1244 0.7925 0.7743 3.1975 1.1921 Callinectessimilis Megalops 0.2021 0.4055 1.7750 3.4148 0.5922 Penaeidae Post Larvae 0.5007 0.2256 0.0402 3.3174 0.3369 Farfantepenaeus sp. Post Larvae 0.0367 0.0438 0.6589 0.0951 0.0521 0.0201 0.6720 0.4026 0.8846 0.0228 0.5633 0.2753 Penaeidae Mysis 0.0184 0.0209 0.1267 0.1585 0.0174 0.5624 0.4394 0.1249 0.2027 1.5031 0.3313 0.2600 Penaeidae Protozoea 0.0735 0.2921 0.1752 0.9667 0.2603 0.7431 0.0775 0.0694 0.1290 0.1325 0.2553 Farfantepenaeus sp. Mysis 0.1653 2.3930 0.0258 0.2477 Farfantepenaeusaztecus Post Larvae 0.4960 0.4088 0.1014 0.4754 0.0174 0.0803 0.2585 0.2082 0.1491 0.1969 Farfantepenaeusduorarum Post Larvae 0.4042 0.0209 0.2044 0.1014 0.2377 0.8529 0.1110 0.2319 0.1707 Penaeidae Other 0.2572 0.1743 0.4581 0.0892 Portunidae Zoea 0.1460 0.3294 0.1215 0.0258 0.0737 0.2154 0.0692 Lepidopa websteri Zoea 0.2256 0.4792 0.0600 Portunidae Megalops 0.1521 0.5169 0.1474 0.0166 0.0538 Scyllarus americanus Phyllosoma 0.0292 0.4754 0.0278 0.0228 0.0538 Menippe mercenaria Megalops 0.2940 0.0209 0.0253 0.0158 0.0347 0.0972 0.0553 0.0477 Lepidopasp. Zoea 0.0551 0.0507 0.1607 0.0555 0.1290 0.0497 0.0415 Rimapenaeus constrictus Mysis 0.0253 0.3803 0.0166 0.0400 Rinzapenaeus constrictus Post Larvae 0.1470 0.0507 0.0158 0.0833 0.0166 0.0277 Farfantepenaeussp. Other 0.1943 0.0215 Eineritatalpoida Juvenile 0.0919 0.0475 0.0555 0.0185 Emerita talpoida Megalops 0.1268 0.0139 0.0138

SLI SL2 SL3 Taxa LifeStage Summer Winter Summer Winter Summer Winter Mean

____ ____ ____ ____CPUE Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Callinectessirnilis Juvenile 0.0367 0.0951 0.0123 Callinectessp. Juvenile 0.0139 0.0455 0.0046 Sicyoniidae Mysis 0.0201 0.0455 0.0046 Sicyoniidae Post Larvae 0.0278 0.0228 0.0046 Callinectes sapidus Juvenile 0.0367 0.0031 Hippidae Zoea 0.0292 0.0031 Menippe sp. Zoea 0.0417 0.0031 Sicyonia sp. Mysis 0.0517 0.0031 Sicvonia sp. Post Larvae 0.0174 0.0166 0.0031 Callinectes sp. Post Larvae 0.0146 0.0015 Hippoidea Zoea 0.0258 0.0015 Menippe sp. Megalops 0.0209 0.0015 Scyllaridae Phyllosoma 0.0209 0.0015 Sievonia brevirostris Mysis 0.0184 0.0015 Sicyonia sp. Juvenile 0.0166 0.0015 Squillidae Pseudozoea 0.0166 0.0015 Unidentified shrimp Post Larvae 0.0158 0.0015 Xiphopenaeus kroyeri Mysis I 1 0.0166 0.0015 Crustacean Total 111.0784 7.5318 1 12.0000 113.35531 19.5563 1 8.8164 [ 22.9564 1 7.9607 [ 12.5069 I 10.63401 9.4284 112.88931 12.5767 Fish, Unidentifiedeggs Egg 9.6822 116.5867 42.2628 18.7532 10.5547 129.9896 46.7765 12.0445 86.6880 45.2267 54.7483 24.4036 51.1883 Clupeidae Egg 2.5037 5.6350 1.5459 117.3898 14.5411 3.5151 0.0139 0.4976 30.2209 0.4307 14.7241 Clupeidae Post Yolk-Sac Larvae 1.1942 0.5007 13.5036 6.2088 2.9002 3.5057 1.6067 5.6604 0.4442 2.0273 0.3644 0.1657 3.2472 Clupeiformes Egg 14.7737 1.5567 Microgobius thalassinus Post Yolk-Sac Larvae 2.3149 0.0209 0.0253 6.6086 0.0347 0.2360 0.0921 0.0228 0.0166 0.8783 Synodontidae Egg 0.0438 2.2662 0.4619 0.1292 1.5825 1.9586 0.5753 Gobiidae Post Yolk-Sac Larvae 0.6430 0.0835 1.5620 0.2027 0.3645 0.5033 1.7674 0.3877 0.0833 0.2212 0.3416 0.1325 0.5384 Sciaenidae Post Yolk-Sac Larvae 0.0919 0.8971 0.0292 0.7856 0.3011 0.4339 0.4419 0.6979 0.1249 0.2764 2.1180 0.3810 0.4830 Engraulidae Egg 1.6168 0.4308 1.3788 0.0139 0.2969 Unidentified fragment Post Yolk-Sac Larvae 0.0919 0.0209 0.3942 0.1774 0.3011 0.4017 1.2148 0.0416 0.2050 0.4639 0.2553

SLI SL2 SL3 Winter Summer Winter Summer Winter Mean Summer CPUE Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post 2.9947 0.2507 0.0367 0.3942 0.1521 0.1109 0.6226 0.3887 0.9793 0.2215 0.1837 0.9180 0.7448 0.2083 0.0278 0.2764 0.0228 0.1657 0.2169 0.0551 0.0626 0.6423 0.5554 0.1205 1 0.1292 0.6377 0.1861 4 + 4 4 4 4 a .4~ 4.

Blenniidae Post Yolk-Sac Larvae 0.0209 0.1314 0.0253 0.0634 0.0868 0.1004 1 0.2843 0.0694 0.4976 0.4099 0.2816 0.1584 Gobiosomarobustunz Post Yolk-Sac Larvae 0.0551 0.1460 0.0146 0.4754 0.1388 0.6829 0.1551 0.0553 0.0911 0.0497 0.1523 Etruneus teres Post Yolk-Sac Larvae 1.0597 0.7455 0.1323 Anchoa sp. Post Yolk-Sac Larvae 0.2086 0.0507 0.3124 0.4792 0.0861 Haemulidae Post Yolk-Sac Larvae 0.0184 0.1043 0.0634 0.0347 0.0201 0.9563 0.0369 0.0228 0.0166 0.0831 Clupeidae Yolk-Sac Larvae 0.5216 0.2628 0.0661 Brevoortiasinithi Post Yolk-Sac Larvae 0.1606 0.0402 0.6377 0.0631 Cynoscion sp. Post Yolk-Sac Larvae 0.0551 0.0626 0.1014 0.1743 0.0803 0.1474 0.0911 0.0569 Microdesmidae Post Yolk-Sac Larvae 0.4596 0.0258 0.0694 0.0538 Perciformes Post Yolk-Sac Larvae 0.3066 0.0507 0.0174 0.0402 0.0139 0.0184 0.0683 0.0331 0.0508 Sparidae Egg 0.4672 0.0492 Anchoa mitchilli Post Yolk-Sac Larvae 0.4409 0.0253 0.0792 0.0461 Eucinostomnus sp. Post Yolk-Sac Larvae 0.1470 0.0438 0.0760 0.0158 0.0139 0.0184 0.1988 0.0446 Carangidae Post Yolk-Sac Larvae 0.1878 0.0158 0.1215 0.0201 0.0416 0.0184 0.0228 0.0354 Diodontidae Post Yolk-Sac Larvae 0.0209 0.2536 0.0347 0.0258 0.0369 0.0166 0.0354 Unidentified fish - damaged Post Yolk-Sac Larvae 0.0209 0.0253 0.0521 0.2068 0.0737 0.0994 0.0354 Larimusfasciatus Post Yolk-Sac Larvae 0.0184 0.0507 0.3011 0.0338 Ctenogobiusboleosonia Post Yolk-Sac Larvae 0.0184 0.1267 0.0174 0.1034 0.1657 0.0323 Gobiosoina sp. Post Yolk-Sac Larvae 0.0919 0.1426 0.0833 0.0228 0.0323 Sparidae Post Yolk-Sac Larvae 0.0184 0.0584 0.1521 0.0158 0.0694 0.0553 0.0166 0.0308 Clupeiformes Post Yolk-Sac Larvae 0.0367 0.0803 0.2277 0.0246 Dactyloscopidae Post Yolk-Sac Larvae 0.1268 0.0803 0.0184 0.0683 0.0246 Achirus lineatus Post Yolk-Sac Larvae 0.0367 0.0209 0.1109 0.0347 0.0402 0.0184 0.0231 Sciaenidae Yolk-Sac Larvae 0.0876 0.0158 0.1822 0.0231 Bathygobius soporator Post Yolk-Sac Larvae 0.0292 0.0507 0.0475 0.0201 0.0139 0.0228 0.0663 0.0215

SLI SL2 SL3 Taxa LifeStage Summer Winter Summer Winter Summer Winter Mean CPUE Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Bregmacerotidae Post Yolk-Sac Larvae 0.0292 0.0521 0.0184 0.0228 0.0994 0.0200 Labridae Post Yolk-Sac Larvae 0.0209 0.0292 0.0253 0.0775 0.0278 0.0369 0.0331 0.0200 Tetraodontidae Post Yolk-Sac Larvae 0.0735 0.0209 0.0507 0.0228 0.0828 0.0200 Chloroscombruschr.'surus Post Yolk-Sac Larvae 0.0174 0.1527 0.0185 Lutjanidae Post Yolk-Sac Larvae 0.0507 0.0174 0.0201 0.1551 0.0369 0.0185 Luq'anus sp. Post Yolk-Sac Larvae 0.1909 0.0184 0.0185 Monacanthidae Post Yolk-Sac Larvae 0.0292 0.0792 0.0803 0.0184 0.0185 Anchoa Ivolepis Post Yolk-Sac Larvae 0.2021 0.0169 Cvnoscion nebulosus Post Yolk-Sac Larvae 0.0626 0.0475 0.0402 0.0184 0.0455 0.0169 Gerreidae Post Yolk-Sac Larvae 0.1168 0.0158 0.0258 0.0139 0.0169 Myctophidae Post Yolk-Sac Larvae 0.1267 0.0347 0.0139 0.0184 0.0331 0.0169 Paralichthyidae Post Yolk-Sac Larvae 0.0184 0.0507 0.0347 0.0803 0.0331 0.0169 Cerdalefloridana Post Yolk-Sac Larvae 0.1736 0.0154 Citharichthysspilopterus Post Yolk-Sac Larvae 0.0184 0.1551 0.0497 0.0154 MIicropogonias undulatus Post Yolk-Sac Larvae 0.0367 0.1022 0.0158 0.0154 Pareques sp. Post Yolk-Sac Larvae 0.0184 0.0317 0.0555 0.0553 0.0154 Cynoscion regalis Post Yolk-Sac Larvae 0.0919 0.0292 0.0517 0.0138 Unidentified fish Post Yolk-Sac Larvae 0.1460 0.0258 0.0184 0.0138 Bairdiella chrysoura Post Yolk-Sac Larvae 0.0209 0.0521 0.0258 0.0497 0.0123 Sciaenops ocellatus Post Yolk-Sac Larvae 0.0735 0.0146 0.0317 0.0139 0.0123 Serranidae Post Yolk-Sac Larvae 0.0438 0.0253 0.0775 0.0184 0.0123 Sparisomna sp. Post Yolk-Sac Larvae 0.0209 0.0292 0.0184 0.0455 0.0331 0.0123 Stomiiformes Post Yolk-Sac Larvae 0.0584 0.0369 0.0455 0.0123 Eleotridae Post Yolk-Sac Larvae 0.0209 0.0994 0.0108 Gobiesoxstrumosus Post Yolk-Sac Larvae 0.0367 0.0317 0.0402 0.0184 0.0108 Gonostomatidae Post Yolk-Sac Larvae 0.0184 0.0209 0.0584 0.0184 0.0108 Harengulajaguana Post Yolk-Sac Larvae 1 0.0972 0.0108 Ophidiiformes Post Yolk-Sac Larvae 0.0209 0.0792 0.0174 0.0108 Ostraciidae Post Yolk-Sac Larvae 0.0584 0.0317 0.0166 0.0108 Anguilloidei Post Yolk-Sac Larvae 0.0951 0.0092

SLI SL2 SL3 Taxa LifeStage Summer Winter Summer Winter Summer Winter Mean CPUE Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Apogonidae Post Yolk-Sac Larvae 0.0292 0.0803 0.0092 Prionotus sp. Post Yolk-Sac Larvae 0.0139 0.0228 0.0663 0.0092 Brevoortia sp. Post Yolk-Sac Larvae 0.0317 0.0683 0.0077 Menticirrhus amnericanus Post Yolk-Sac Larvae 0.0584 0.0201 0.0077 Mlicropogoniasfiirnieri Post Yolk-Sac Larvae 0.0730 0.0077 Monacanthus ciliatus Post Yolk-Sac Larvae 0.0417 0.0174 0.0369 0.0077 Ophidion sp. Post Yolk-Sac Larvae 0.0475 0.05 17 0.0077 Parablenniusmarmoreus Post Yolk-Sac Larvae 0.0584 0.0184 0.0077 Parequesacuminatus Post Yolk-Sac Larvae 0.0174 0.0803 0.0077 Scaridae Post Yolk-Sac Larvae 0.0367 0.0317 1.0000 0.0077 Selene setapinnis Post Yolk-Sac Larvae 0.1004 0.0077 Stellifer lanceolatus Post Yolk-Sac Larvae 0.1004 0.0077 Svmphurus sp. Post Yolk-Sac Larvae 0.0367 0.0292 0.0158 0.0077 Syngnathus louisianae Juvenile 0.0184 0.0584 0.0077 Diplospinus multistriatus Post Yolk-Sac Larvae 0.0663 0.0062 Pomacentridae Post Yolk-Sac Larvae 0.0209 0.0292 0.0201 0.0062 Sauridabrasiliensis Post Yolk-Sac Larvae 0.0184 0.0475 0.0062 Scarus sp. Post Yolk-Sac Larvae 4.0000 0.0062 Selar crumenophthalmnus Post Yolk-Sac Larvae 0.0803 0.0062 Sphyraenidae Post Yolk-Sac Larvae 0.0584 0.0062 Tetraodontidae Yolk-Sac Larvae 0.0584 0.0062 Balistidae Post Yolk-Sac Larvae 0.0497 0.0046 Chaetodipterusfaber Post Yolk-Sac Larvae 0.0347 0.0228 0.0046 Labrisomidae Post Yolk-Sac Larvae 0.0253 0.0174 0.0139 0.0046 Menticirrhus sp. Post Yolk-Sac Larvae 0.0367 0.0184 0.0046 Ophidion sp. Yolk-Sac Larvae 0.0475 0.0046 Pleuronectiformes Post Yolk-Sac Larvae 0.0603 0.0046 Scorpaenidae Post Yolk-Sac Larvae 0.0417 0.0184 0.0046 Sphoeroides sp. Post Yolk-Sac Larvae 0.0292 0.0253 0.0046 A/uterus schoepfii Post Yolk-Sac Larvae 0.0174 0.0184 0.0031

SLI SL2 SL3 Mean LifeStage Summer Winter Summer Winter Summer Winter Ce Taxa Post Pre Post Pre Post Pre Post Pre Post Pre Post Pre Atheriniformes Post Larvae 0.0367 0.0031 Brevoortia Ivrannus Post Yolk-Sac Larvae 0.0517 0.0031 Chasmnodes saburrae Post Yolk-Sac Larvae 0.0158 0.0201 0.0031 Clupeiformes Yolk-Sac Larvae 0.0455 0.0031 Diplectrunz sp. Post Yolk-Sac Larvae 0.0367 0.0031 Elops saurus Post Yolk-Sac Larvae 0.0258 0.0166 0.0031 Epinephzehis sp. Post Yolk-Sac Larvae 0.0367 0.0031 Gobiosomna bosc Post Yolk-Sac Larvae 0.0517 0.0031 Hygophurn reinhardtii Post Yolk-Sac Larvae 0.0292 0.0031 Leiostonus xanthurus Post Yolk-Sac Larvae 0.0209 0.0146 0.0031 Microdesmus sp. Post Yolk-Sac Larvae 0.0367 0.0031 Mullidae Post Yolk-Sac Larvae 0.0331 0.0031 Opistognathidae Post Yolk-Sac Larvae 0.0292 0.0031 Percophidae Post Yolk-Sac Larvae 0.0507 0.0031 Triglidae Post Yolk-Sac Larvae 0.0158 0.0201 0.0031 Acanthurus sp. Post Yolk-Sac Larvae 0.0139 0.0015 Achiridae Egg 0.0184 0.0015 Acropomatidae Post Yolk-Sac Larvae 0.0139 0.0015 Astrapogonpuncticidatus Post Yolk-Sac Larvae 0.0253 0.0015 Atheriniformes Post Yolk-Sac Larvae 0.0139 0.0015 Atherinopsidae Post Yolk-Sac Larvae 0.0201 0.0015 Bothidae Post Yolk-Sac Larvae 0.0258 0.0015 Bramidae Post Yolk-Sac Larvae 0.0253 0.0015 Citharichtthysarctifrons Post Yolk-Sac Larvae 0.0228 0.0015 Citharichthvs sp. Juvenile 0.0158 0.0015 Congridae Post Yolk-Sac Larvae 0.0184 0.0015 Cornphaenahippurus Post Yolk-Sac Larvae 0.0258 0.0015 Ctenogobius sp. Post Yolk-Sac Larvae 0.0253 0.0015 Cyclothone sp. Post Yolk-Sac Larvae 0.0139 0.0015 Diodon sp, Post Yolk-Sac Larvae 1 0.0184 0.0015

SLU SL2 SL3 Taxa LifeStage Summer Winter Summer Winter Summer Winter Mean CPUE Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Diogenichthysatlanticus Post Yolk-Sac Larvae 0.0253 0.0015 Dormitator maculatus Post Yolk-Sac Larvae 0.0174 0.0015 Eleotrispisonis Post Yolk-Sac Larvae 0.0166 0.0015 Engraulidae Yolk-Sac Larvae 0.0184 0.0015 Euthvnnus alletteratus Post Yolk-Sac Larvae 0.0174 0.0015 Gempylidae Post Yolk-Sac Larvae 0.0174 0.0015 Gobionellusoceanicus Post Yolk-Sac Larvae 0.0184 0.0015 Gobiosomaparri Post Yolk-Sac Larvae 0.0253 0.0015 Haemulon sp. Post Yolk-Sac Larvae 0.0146 0.0015 lHlippocampus erectus Post Yolk-Sac Larvae 0.0209 0.0015 Lupinoblennius nicholsi Post Yolk-Sac Larvae 0.0184 0.0015 Melamphaidae Post Yolk-Sac Larvae 0.0253 0.0015 Melanocetidae Post Yolk-Sac Larvae 0.0184 0.0015 Merlucciidae Post Yolk-Sac Larvae 0.0174 0.0015 AIugil cephalus Post Yolk-Sac Larvae 0.0209 0.0015 Nes longus Post Yolk-Sac Larvae 0.0184 0.0015 Ogcocephalhs sp. Post Yolk-Sac Larvae 0.0174 0.0015 Ophidiidae Post Yolk-Sac Larvae 0.0184 0.0015 Paralepididae Post Yolk-Sac Larvae 0.0209 0.0015 Phosichthyidae Post Yolk-Sac Larvae 0.0201 0.0015 Pomacanthidae Post Yolk-Sac Larvae 0.0184 0.0015 Scomberonmorus maculatus Post Yolk-Sac Larvae 0.0158 0.0015 ScorpaenifoTrmes Post Yolk-Sac Larvae 0.0209 0.0015 Stephanoberyciformes Post Yolk-Sac Larvae 0.0209 0.0015 Syngnathidae Post Yolk-Sac Larvae 0.0184 0.0015 Syngnathus louisianae Post Yolk-Sac Larvae 0.0166 0.0015 Syngnathus sp. Post Yolk-Sac Larvae 0.0139 0.0015 Tetraodontiformes Egg 0.0166 0.0015 Trichiuruslepturus Post Yolk-Sac Larvae 0.0258 0.0015 Umbrina coroides Post Yolk-Sac Larvae 0.0253 0.0015

SLU SL2 SL3 Taxa LifeStage Summer Winter Summer Winter Summer Winter Mean CPUE Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Unidentified fragment Yolk-Sac Larvae 0.0139 0.0015 Uranoscopidae Post Yolk-Sac Larvae 0.0253 0.0015 Fish Total 120.7974 1123.4926 81.9708 129.6503 1 28.6371 1254.32141 68.8291 127.4490o 90.4498 150.68191 93.8511 128.7276 76.7694 Total 131.8758 1131.02441 93.9708 143.00561 48.1933 1263.13781 91.7855 135.40971102.95671 61.31591103.27941 41.6170189.3461

Table 64. Mean CPUE (Number Collected Per 100m 3 of Water Filtered) for Fish and CRI Decapod Crustacean Larvae and Fish Eggs Captured in Plankton Tows, for All Events, by Pre- and Post-Uprate, Season, and Area, St. Lucie Plant EPU.

Season Pre-Post-_

Uprate IIII SLU SL2 SL3 Total Pre 31.88 48.19 102.96 64.32 Summer Post 131.02 263.14 61.32 154.99 Pre 93.97 91.79 103.28 95.82 Winter Post 43.01 35.41 41.62 40.28 Total 1 76.78 115.41 J 77.16 89.35

Table 65. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Number of Taxa and Catch Per Unit Effort (CPUE) Among Areas for Larval Fish, Fish Eggs and Commercially/

Recreationally Important (CRI) Invertebrates Among Areas by For Fauna Captured by Plankton Tows During All Events, Seasons, Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU.

Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Fish Larvae Taxa 7.50 7.43 7.61 All Events 2 84 0.0914 0.9553

+/- 4.32 +/- 4.37 +/- 4.66 7.19 6.18 6.00 Pre-uprate + 5.21 + 4.64 +/- 2.88 2 48 0.4839 0.7851 7.92 8.25 9.75 Post-uprate +/- 2.94 + 4.03 + 5.77 2 36 0.5289 0.7676 6.81 7.63 6.56 Summer 2 48 0.6349 0.7280

+/- 4.53 +/-5.14 +4.10 8.42 7.17 9.00 Winter 2 36 0.6889 0.7086

+/-4.03 +3.27 +5.17 Fish Larvae CPUE 0.10 0.08 0.04 All Events 2 84 2.3530 0.3084

+/- 0.23 +/- 0.10 + 0.03 0.12 0.08 0.03 Pre-uprate 2 48 2.4731 0.2904

+ 0.29 +/- 0.11 + 0.03 0.06 0.79 0.04 Post-uprate + 0.10 + 0.08 + 0.02 2 36 1.3393 0.5119 0.05 0.09 0.03 Summer 2 48 3.5142 0.1725

+/- 0.40 +0.11 +0.02 0.17 0.07 0.05 Winter 2 36 0.0060 0.9970

+/- 0.34 + 0.08 + 0.02

Fish Egg Taxa 1.57 1.57 1.93 All Events 2 84 2.5624 0.2777

+/- 0.92 +/- 0.84 + 0.98 1.63 1.63 2.25 Pre-uprate + 1.02 +/- 0.96 _+1.06 2 48 4.1373 0.1264 1.50 1.50 1.50 Post-uprate 2 36 0.0224 0.9889

+ 0.80 +/- 0.67 + 0.67 1.13 1.25 1.75 Summer 2 48 5.4968 0.0640

+ 0.62 +/- 0.77 + 0.86 2.17 2.00 2.17 Winter 2 36 0.1789 0.9144

+/- 0.94 +/- 0.74 +/-1.11 Fish Egg CPUE All Events 0.54 0.77 0.68 2 84 1.3069 0.5203

+/- 0.91 +/-2.44 +/- 1.17 Pre-uprate 0.39 0.34 0.89 2 48 1.6666 0.4346

+/- 0.49 +/- 0.56 +/- 1.47 0.74 1.35 0.41 Post-uprate +/- 1.27 + 3.67 +/- 0.48 2 36 0.1547 0.9256 0.59 0.99 0.78 Summer 2 48 0.6327 0.7288

+/- 1.15 +/-3.21 +/-1.49 0.48 0.48 0.56 Winter 2 36 1.0150 0.6020

+/- 0.46 +/- 0.61 +/- 0.54

CRI Crustacean Taxa 4.32 4.68 5.46 All Events 2 84 2.9238 0.2318

+/- 1.89 +/- 1.99 +/- 2.36 4.31 4.63 5.63 Pre-uprate 2 48 3.2695 0.1950

+/- 1.85 +/- 2.22 + 2.53 4.33 4.75 5.25 Post-uprate 2 36 0.6603 0.7188

+ 2.02 +/- 1.71 + 2.22 4.31 4.81 5.94 Summer 2 48 3.3973 0.1829

+ 1.92 +/-2.14 +/-2.52 4.33 4.50 4.83 Winter 2 36 0.1840 0.9121

+/- 1.92 + 1.83 +/- 2.08 CRI Crustacean CPUE 0.11 0.16 0.12 All Events 2 84 0.8931 0.6398

+/-0.11 +/-0.19 +/-0.11 0.12 0.21 0.12 Pre-uprate 2 48 1.0554 0.5900

+/- 0.12 +/- 0.23 +/- 0.13 0.09 0.08 0.12 Post-uprate 2 36 2.7613 0.2514

- 0.10 +/- 0.09 +/- 0.09 0.09 0.16 0.11 Summer 2 48 0.8284 0.6609

+/- 0.06 +/- 0.18 +/- 0.09 0.13 0.16 0.15 Winter 2 36 1.3108 0.5192

+/- 0.15 + 0.22 - 0.14

Table 66. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Number of Taxa and Catch Per Unit Effort (CPUE) Among Pre- and Post-Uprate Events Within Each Area for Larval Fish, Fish Eggs and Commercially/Recreationally Important (CRI) Invertebrates Captured by Plankton Tows, St. Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Fish Larvae Taxa 7.19 7.92 SLI 7 7.92 1 28 0.7868 0.3751

+/- 5.21 + 2.94 6.81 8.25 SL2 1 28 1.3094 0.2525

+ 4.64 +/- 4.03 6.00 9.75 SL3 28 2.8334 0.0923

• 2.88 +/- 5.77 Fish Larvae CPUE 0.12 0.06 SLI 1 28 0.8621 0.3532

+/- 0.29 +/- 0.10 0.08 0.08 SL2 1 28 0.3104 0.5774

+/-0.11 +/-0.08 0.03 0.04 SL3 1 28 0.9504 0.3296

+/- 0.03 + 0.02 Fish Egg Taxa Sma p l i n g ! M e .D n V l e P r A e .

Fish Egg CPUE SLI +/--0.49 +/- 1.27 1 28 0.9060 0.3412 0.34 1.35 SL2 1 28 1.4573 0.2274 40.56 4 3.67 0.89 0.41 SL3 1 28 0.1379 0.7103

• 1.47 +/- 0.48 CRI Crustacean Taxa 4.31 4.33 SLI 28 0.0050 0.9436 0 1.85 +/-2.02 4.63 4.75 SL2 1 28 0.0810 0.7759 2.22 01.71 5.63 5.25 SL2 28 0.2220 0.6375

+/- 2.53 + 2.22 CRI Crustacean CPUE 0.12 0.09 SL1 1 28 0.3642 0.5462

+/- 0.12 +-0.10 0.21 0.08 SL2 128 1.9397 0.1637

+/- 0.23 +0.09 0.12 0.12 SL3 128 0.1056 0.7452 4-0.13 +0.09

Table 67. CPUE (Number Collected Per 100m 3 of Water Filtered) By Area for All Fish Larvae Captured in Plankton Tows, for All Events, by Season, and Pre- and Post-Uprate, St. Lucie Plant EPU.

Season Post-Uprate SLU SL2 SL3 Mean CPUE Pre 6.45 14.33 1.76 7.29 Summer Post 4.40 6.94 4.96 5.51 Winter Pre 23.28 6.43 5.94 13.42 Winter Post 8.77 11.76 3.88 7.47 Mean CPUE 10.31 9.94 3.86 8.42

Table 68. CPUE (Number Collected Per 100m 3 of Water Filtered) By Area for All Fish Eggs Captured in Plankton Tows, for All Events, by Season, and Pre-and Post-Uprate, St. Lucie Plant EPU.

Season T Post-Uprate IIII SL2 S Mean CPUE Pre 14.35 14.31 88.69 42.59 Summer Post 119.09 247.38 45.72 140.44 Pre 63.58 62.40 87.91 69.80 Winter Post 20.88 15.69 24.85 21.16 Mean CPUE 55.48 90.21 61.72 68.87

Table 69. CPUE (Number Collected Per 100m 3 of Water Filtered) By Area for All Commercially or Recreationally Important (CRT) Decapod Crustaceans Captured in Plankton Tows, for All Events, by Season, and Pre- and Post-Uprate, St. Lucie Plant EPU.

Season Summer Pre- Post-Uprate Pre 1_

SL 11.08 t SL2 19.56 SL3 12.51 IMean CPUE 14.44 Post 7.53 8.82 10.63 9.05 Pre 12.00 22.96 9.43 14.67 Winter Post 13.36 7.96 12.89 11.65 Mean CPUE 11.00 15.26 11.58 12.58

Table 70. Number of Green Turtles (Chelonia mydas) Sighted per Kilometer of Transect Surveyed, for All Events, by Pre- and Post- Uprate, Season, and Area, St. Lucie Plant EPU.

Season Post-Uprate SLI SL2 SL3 Mean Pre 0.40 2.40 0.00 0.93 Summer Post 1.00 1.50 0.00 0.83 Pre 0.33 3.50 0.00 1.28 Winter Post 1.33 2.83 0.17 1.44 Mean 0.71 2.54 0.04 1.10

Table 71. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Total Number of Green Turtles Observed Among Areas During All Events, Seasons, Pre- and Post-Uprate Sampling Events, St. Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Total Green Turtles Observed Per Area 19.64 All Events 2.79 + 20.76 0.71 2 42 21.8728 < 0.0001

+4.37 +2.67 21.38 Pre-uprate 0.88 +/- 22.06 0.00 2 24 17.9747 0.0001

+ 0.99 + 0.00 5.33 17.33 1.67 Post-uprate 2 18 5.7321 0.0569

+ 5.89 + 20.69 +/- 4.08 13.75 Summer 2.13 +/- 17.87 0.00 2 24 14.2193 0.0008

+3.31 -0.00 27.50 Winter 3.67 +/- 23.32 1.67 2 18 8.2004 0.0166

+ 5.72 +/- 4.08

Table 72. Results of Statistical Comparisons (Kruskal-Wallis ANOVA) of Total Number of Green Turtles Observed Among Pre- and Post-Uprate Events Within Each Area, St.

Lucie Plant EPU. Means Are Presented For Each Area Plus or Minus One Standard Deviation (SD).

Total Green Turtles Observed Per Area 0.88 5.33 SLI 1 14 3.2828 0.0700

-0.99 +5.89 21.38 17.33 SL2 1 14 0.1510 0.6976

- 22.06 +/- 20.69 0.00 1.67 SL3 1 14 1.3333 0.2482

+/- 0.00 4 4.08

Appendix Figure 1. Mean dissolved oxygen in surface water among sampling areas (all 3 transects combined), during daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means.

Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

8.0 *Area SLU

-Area SL2 7.5 :Area SL3 7.0 a aa S6.5 a O

Caa F 6.0aa aa 0a 5.5 5.0 C 4.5 4.0 Aug-11 Dec-11 Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-ll Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13 Appendix Figure 2. Mean dissolved oxygen in bottom water among sampling areas (all 3 transects combined), during daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means.

Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

8.0 -*SArea SLU aa  :ýi::Area SL2 7.5 a a :4*Area SL3 7.0 a ab aaa 6.5 a a a a aa aa b

-o F 6.0 0a 5.5 a 5.0 4.5 4.01 Aug-1i Dec-11 Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-ll Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13

Appendix Figure 3. Mean dissolved oxygen in surface water among sampling areas (all 3 transects combined), during night time trawling over 14 sampling events (Aug 2011 - Dec 2013),

St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

7.4 5Area SLI aArea 72 SL2 7.2 a*Area SL3 7.0 a aa aa a 6.8

• ,a a

a. ab E6.6 aa a a C ab T o 6.0 0 6.2

'a (D

b

-5 6.0 o 5.8 5.6 aa 5.4 a

5.2 Aug-i1 Dec-1I Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-li Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13 Appendix Figure 4. Mean dissolved oxygen in bottom water among sampling areas (all 3 transects combined), during night time trawling over 14 sampling events (Aug 2011 - Dec 2013),

St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

8.0 EEArea SL1 EArea SL2 aEArea SL3 7.5 aal a* a

-7.0 a E7 aa0 a a*__

6.5 b 'a 0 a a

" 6 .0 a 5.5 5.0 a a a

4.5 Aug-11 Dec-11 Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-11 Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13

Appendix Figure 5. Mean pH in surface water among sampling areas (all 3 transects combined),

during daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

8.4 - EEArea SLI a  :ýi*Area SL2 8.3 a a --ý-Area SL3 8.2 a at: aa a a a aa

• aaa 8.0 8.1a 8.0 7.88 fl 7.9 7.8 ' , a b a 7.7 a a

7.6 b

7.5 Aug-I1 Dec-11 Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-l1 Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13 Appendix Figure 6. Mean pH in bottom water among sampling areas (all 3 transects combined),

during daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

8.5  :: -Area SLI EArea SL2 8.4  ::*Area SL3 a

8.3 8.2 aaa aa b 8.1 a

- 8.0 7.9 abb 7.8 7.7 b

7.6 b

7.5 Aug-I1 Dec-11 Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-ll Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13

Appendix Figure 7. Mean pH in surface water among sampling areas (all 3 transects combined),

during night time trawling over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU.

Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

8.4 ]Area SLU PArea SL2 8.3 :Area SL3 a

a aa aa a aa 8.1 a a atb a a aba ab b ba 7.8 b 7.7 a

a a 7.6 Aug-lI Dec-l1 Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-l1 Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13 Appendix Figure 8. Mean pH in bottom water among sampling areas (all 3 transects combined),

during night time trawling over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU.

Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

8.4 :Area SL1

=Area SL2 8.3 =Area SL3 a

82aa. a aa a a aa 8.2a 8.1 a a a b a S8.0 b aa a 7.9 b b

7.8 b

7.7 aaa 7.6, Aug-11 Dec-lI Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-l1 Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13

Appendix Figure 9. Mean salinity in surface water among sampling areas (all 3 transects combined), during daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St.

Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

37.5  :-Area SLU

- -Area SL2 a*Area SL3 37.0 a,

36. a ab a aa a aa

(: (363.5 36.0 aa a b ~a b 35.0*f 3455 34.5 34.0 Aug-11 Dec-11 Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-li Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13 Appendix Figure 10. Mean salinity in bottom water among sampling areas (all 3 transects combined), during daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St.

Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

37.5 =5Area SLO

=Area SL2 a aaArea SL3 37.0 365 aa a a a.. a a aaa 0 a 36.0 Z_ a aa CO a ab 35.0 34.5 34.0 Aug-11 Dec-1I Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-li Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13

Appendix Figure 11. Mean salinity in surface water among sampling areas (all 3 transects combined), during night time trawling over 14 sampling events (Aug 2011 - Dec 2013), St.

Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

37.2 ýEArea SLU a ;Area SL2 37.0 aa :T--Area SL3 a aa 36.6 aa a 36.6'a 36.4 b a a

._ 36.2 bo a aa a 36.0 a ab a a .

35.8 a a a 35.6 a b 35.41 Aug-1I Dec-I1 Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-ll Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13 Appendix Figure 12. Mean salinity in bottom water among sampling areas (all 3 transects combined), during night time trawling over 14 sampling events (Aug 2011 - Dec 2013), St.

Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

37.2  :ýýArea SLU RPArea SL2 37.0 Z*Area SL3 Ba aa a S a aa 36.8 aBaa a a a a 36.6 S a o9 36.4 b b a a a

- 36.2 cca a a' a 36.0 *a a a a 35.8 a B b

35.6 b 35.4 ,

Aug-1I Dec-1I Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-ll Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13

Appendix Figure 13. Mean specific conductivity in surface water among sampling areas (all 3 transects combined), during daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means.

Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

56.5 TArea SLO 5: Area SL2 56.0 a *Area SL3 a a 55.5 aa a

-55.0 E

54.5

' 54.0 I. b a C b

( 53.5 5

I.

b

• . 53.0 C,, I.

52.5 I 52.0 [ C 51.5 Aug-11 Dec-1I Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-ll Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13 Appendix Figure 14. Mean specific conductivity in bottom water among sampling areas (all 3 transects combined), during daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means.

Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

56.5 :r -Area SLU ZArea SL2 56.0 I :FArea SL3 a

a a 55.5 I

-55.0 I. a CO)

E v 54.5 I.

b

, 54.0 I. a b b 3 53.5 I.

COL53.0 CO 52.5 52.0 C 51.5 Aug-11 Dec-11 Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-11 Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13

Appendix Figure 15. Mean specific conductivity in surface water among sampling areas (all 3 transects combined), during night time trawling over 14 sampling events (Aug 2011 - Dec 2013),

St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

56.5 *Area SLO 3Area SL2

• 1; Area SL3 56.0 a aa a a 55.5 I E

55.0 I a a

0 a

C., ;a 54.5 [

U) a a b

54.0 F b b

a 53.5 Aug-iI Dec-il Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-l1 Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13 Appendix Figure 16. Mean specific conductivity in bottom water among sampling areas (all 3 transects combined), during night time trawling over 14 sampling events (Aug 2011 - Dec 2013),

St. Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

56.0 r  :;ý:Area SLU

=Area SL2 55.8 L a Area SL3 T a a ad a

55.6 I a aa T TT1a aa S55.4 E 55.2 a

5 55.0 F b 54.8 a 0 I. a

• 54.6 F a

05. 54.4 a

C,, a I. a 54.2 F aa a b 54.0 b

53.8 Aug-11 Dec-1I Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-lI Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13

Appendix Figure 17. Mean temperature in surface water among sampling areas (all 3 transects combined), during daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St.

Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

30 -Area SLU

=::Area SL2 29 -a Area SL3 a a a 28 a a a 27 a a

0 26 b "25 b aa a a C&24 a E a a b b S23 b aaa 22 a

21 20 b c aa 19, Aug-Il Dec-1I Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-lI Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13 Appendix Figure 18. Mean temperature in bottom water among sampling areas (all 3 transects combined), during daytime gill netting over 14 sampling events (Aug 2011 - Dec 2013), St.

Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

30 6Area SL1

• Area SL2 29 a Area SL3 28 a a aa 27 a 26 abb o 25 a Saaaa S24a 0..

E 23 ab 22 21 20 19 18 Aug-11 Dec-11 Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-li Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13

Appendix Figure 19. Mean temperature in surface water among sampling areas (all 3 transects combined), during night time trawling over 14 sampling events (Aug 2011 - Dec 2013), St.

Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

30 --*-Area

• Area SL1 SL2 29 :C*Area SL3 a aa aa 28 27 a 2a ab aa a25 a b a

a 4K24 a b E~21 S23 a a

22 b a aa 21 C a

a 20 a 19 Aug-1Il Dec-li1 Apr-12 Aug-12 Jan-13 Jun-13 Oct-1 3 Oct-il Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13 Appendix Figure 20. Mean temperature in bottom water among sampling areas (all 3 transects combined), during night time trawling over 14 sampling events (Aug 2011 - Dec 2013), St.

Lucie Plant EPU. Vertical bars represent 95% confidence intervals about the means. Letters above each area are used to denote differences among means. The same letter indicates no statistically significant difference (p<0.05), while different letters indicate a significant difference among areas within each sampling event.

30 ý]EArea SLO

§Area SL2 29 FArea SL3 aa aa 28 a 2a a oO27 aa a

'OL26 a a E a2 aa a a a I--23 a

22 a 21 aaaa b 20 a b

19 Aug-il Dec-1i Apr-12 Aug-12 Jan-13 Jun-13 Oct-13 Oct-l1 Feb-12 Jun-12 Oct-12 Mar-13 Aug-13 Dec-13 A