ML24337A196

Discharge Monitoring Report (Dmr), September 2024

ML24337A196
Person / Time
Site:	Sequoyah
Issue date:	12/02/2024
From:	Markum T Tennessee Valley Authority
To:	Office of Nuclear Reactor Regulation
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{{#Wiki_filter:Tennessee Valley Authority, Sequoyah Nuclear Plant, P.O. Box 2000, Soddy Daisy, TN 37384 December 2, 2024 ATTN: Document Control Desk U.S. Nuclear Regulatory Commission Washington, D.C. 20555-0001

Subject:

Sequoyah Nuclear Plant, Discharge Monitoring Report (DMR), September 2024 Attached is the September 2024 DMR for Sequoyah Nuclear Plant. Respectfully, Travis R. Markum Environmental Scientist

Environmental Records Processing Form TVA 20946 [02-20-2019] Page 1 of 1 Title of File SQN 101 WET Report - Sep 2024 Site/Plant/Project Name SQN Accession Number (optional) Work Order Number (optional) Your Name Rick Sherrard Date Submitted (YYYYMMDD) 20241008 Document Date (YYYYMMDD) 20241008 Show Instructions For assistance, please contact the Facility or Site Environmental Contact for your site/project, the Environmental Media Specialists (See Contacts on Environment InsideNet Page), or your Administrative Support Person. Document Type WATER/WASTEWATER Record Type NPDES Permit Compliance Records

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: September 24, 2024

1. Facility / Discharger: Sequoyah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1,579

6. Receiving Stream: Tennessee River (TRM 483.6)

7. 1Q10: 2,456

8. Outfall Tested: 101

9. Dates Sampled: September 08 - 13, 2024

10. Average Flow on Days Sampled (MGD): 968.817, 984.489, 982.473

11. Pertinent Site Conditions: Production / operation data will be provided upon request.

12. Test Dates: September 10 - 17, 2024

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested (%):

Pimephales promelas: UV treated Outfall 101: 17.25, 34.5, 69, 84.5, 100 UV treated Intake: 100 Ceriodaphnia dubia: Non-treated Outfall 101: 17.25, 34.5, 69, 84.5, 100 Non-treated Intake: 100

16. Permit Limit Endpoint (%): Outfall 101: IC25 = 69%

Entered by: J. Perez Reviewed by: J. Sumner Page 1 of 93

17. Test Results: Outfall 101: Pimephales promelas: IC25 > 100%

Ceriodaphnia dubia: IC25 > 100%

18. Facility

Contact:

Travis Markum Phone #: (865) 748-3294

19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Rick Sherrard Phone #: (423) 987-2250

22. Notes: Exposures to samples collected September 08 - 13, 2024 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant differences from the controls during this study period.

Entered by: J. Perez Reviewed by: J. Sumner Page 2 of 93

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101, Intake

2. Sample Type: Composite

3. Sample Information:

Sample ID Date (MM-DD-YY) Time (ET) Collected Date (MM-DD-YY) Time (ET) Received Arrival Temp. (°C) Initial TRC* (mg/L) Date (MM-DD-YY) Time (ET) Last Used By 101 09-08-24 / 0700 to 09-09-24 / 0600 09-09-24 / 1310 1.1 <0.10 09-10-24 / 0838 09-11-24 / 0812 Intake 09-08-24 / 0700 to 09-09-24 / 0600 09-09-24 / 1310 1.1 <0.10 09-10-24 / 0838 09-11-24 / 0812 101 09-10-24 / 0700 to 09-11-24 / 0600 09-11-24 / 1250 1.1 <0.10 09-12-24 / 0810 09-13-24 / 0810 Intake 09-10-24 / 0700 to 09-11-24 / 0600 09-11-24 / 1250 1.4 <0.10 09-12-24 / 0810 09-13-24 / 0810 101 09-12-24 / 0700 to 09-13-24 / 0600 09-13-24 / 1211 1.1, 1.3 <0.10 09-14-24 / 0903 09-15-24 / 0900 09-16-24 / 0804 Intake 09-12-24 / 0700 to 09-13-24 / 0600 09-13-24 / 1211 1.6 <0.10 09-14-24 / 0903 09-15-24 / 0900 09-16-24 / 0804

• TRC = Total Residual Chlorine Samples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0oC) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Entered by: J. Perez Reviewed by: J. Sumner I I I I I I I Page 3 of 93

Pimephales promelas Ceriodaphnia dubia Test Organisms:

1. Source:

In-house Cultures In-house Cultures

2. Age:

< 24-hours old < 24-hours old Test Method Summary:

1. Test Conditions:

Static, Renewal Static, Renewal

2. Test Duration:

7 days Until at least 60% of control females have 3 broods

3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic

4. Number of Replicates:

4 10

5. Organisms per Replicate: 10 1

6. Test Initiation: (Date/Time): 09-10-24 0743 ET 09-10-24 0838 ET

7. Test Termination: (Date/Time): 09-17-24 0602 ET 09-17-24 0647 ET

8. Test Temperature: Outfall 101: Mean = 25.0oC Mean = 25.1oC (24.7 - 25.3oC)

(24.8 - 25.4oC)

9. Physical / Chemical Measurements:

Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA). Entered by: J. Perez Reviewed by: J. Sumner Page 4 of 93

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted September 10 - 17, 2024 using effluent from Outfall 101. Test Solutions (% Effluent) Percent Surviving (time interval used - days) 1 2 3 4 5 6 7 Control, UV-treated 100 100 100 100 100 100 100 17.25% 100 100 100 100 100 100 100 34.5% 100 100 100 100 100 100 100 69% 100 100 100 100 100 100 100 84.5% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100

Control, Non-treated 100 100 100 100 100 100 100 Test Solutions

(% Effluent) Mean Dry Weight (mg) (replicate number) 1 2 3 4 Mean

Control, UV-treated 0.833 0.722 0.858 0.916 0.832 17.25%

0.960 0.907 0.844 0.865 0.894 34.5% 0.870 0.925 0.924 0.853 0.893 69% 0.869 0.838 0.809 0.752 0.817 84.5% 0.902 0.839 0.834 0.818 0.848 100.0% 0.900 0.874 0.680 0.812 0.817 Intake 0.823 0.701 0.826 0.766 0.779 Control, Non-treated 0.842 0.859 0.877 0.906 0.871 IC25 Value: > 100% Permit Limit: 69% 95% Confidence Limits: Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc* Permit Limit: 1.4 TUc

• TUa = 100/LC50: TUc = 100/ IC25 Entered by: J. Perez Reviewed by: J. Sumner I

I I I I Page 5 of 93

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted September 10 - 17, 2024 using effluent from Outfall 101. Test Solutions (% Effluent) Percent Surviving (time interval used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 17.25% 100 100 100 100 100 100 100 34.5% 100 100 100 100 100 100 100 69% 100 100 100 100 100 100 100 84.5% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions (% Effluent) Reproduction (#young/female/7 days) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 31 30 31 32 29 29 33 28 30 30.2 17.25% 30 37 32 31 37 31 34 29 31 32 32.4 34.5% 30 31 35 39 31 35 33 33 37 38 34.2 69% 35 38 35 37 38 38 34 31 33 38 35.7 84.5% 37 33 40 36 37 32 35 36 34 33 35.3 100.0% 36 37 36 36 38 32 39 33 38 37 36.2 IC25 Value: > 100% Permit Limit: 69% 95% Confidence Limits: Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc* Permit Limit: 1.4 TUc

• TUa = 100/LC50: TUc = 100/ IC25 Entered by: J. Perez Reviewed by: J. Sumner Page 6 of 93

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted September 10 - 17, 2024 using water from Intake Test Solutions (% Effluent) Percent Surviving (time interval used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions (% Effluent) Reproduction (#young/female/7 days) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 34 30 28 31 32 30 29 31 31 30 30.6 Intake 35 31 37 38 33 36 33 35 36 32 34.6 IC25 Value: > 100% Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A

• TUa = 100/LC50: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC25) Pimephales promelas September 10 - 17, 2024 0711 7 days KCl 0.71 g/L Ceriodaphnia dubia September 10 - 17, 2024 0813 7 days NaCl 1.11 g/L Entered by: J. Perez Reviewed by: J. Sumner Page 7 of 93

24.7 - 24.9 24.9 - 25.2 7.6 7.8 6.2 7.8 7.31 - 7.75 6.83 - 7.55 298 330 64 64 87 89 24.7 - 24.9 24.9 - 25.3 7.6 7.9 6.3 7.7 7.28 - 7.67 6.85 - 7.57 300 322 64 64 87 95 24.7 - 25.0 24.8 - 25.3 7.6 7.9 6.5 7.7 7.27 - 7.66 6.84 - 7.55 286 305 24.8 - 25.0 24.8 - 25.3 7.7 8.0 6.5 7.7 7.27 - 7.64 6.85 - 7.54 263 283 24.9 - 25.0 25.0 - 25.3 7.7 8.0 6.5 7.7 7.24 - 7.64 6.89 - 7.54 230 242 24.9 - 25.0 24.8 - 25.2 7.7 8.0 6.4 7.7 7.23 - 7.62 6.92 - 7.51 212 222 25.0 - 25.0 24.8 - 25.2 7.8 8.0 6.3 7.7 7.23 - 7.65 6.89 - 7.52 194 204 74 78 83 83 < 0.10 < 0.10 25.0 - 25.0 24.9 - 25.3 7.8 8.1 6.2 7.7 7.20 - 7.66 6.92 - 7.51 189 201 74 78 81 83 < 0.10 < 0.10 24.8 - 24.9 25.1 - 25.3 7.6 7.8 7.6 7.9 7.31 - 7.75 7.23 - 7.61 298 330 64 64 87 89 24.9 - 25.0 25.0 - 25.4 7.7 7.8 7.6 7.9 7.27 - 7.63 7.21 - 7.59 287 309 24.9 - 25.0 25.0 - 25.3 7.7 7.8 7.6 8.0 7.28 - 7.62 7.20 - 7.59 265 290 25.0 - 25.0 25.0 - 25.4 7.7 7.9 7.6 7.9 7.26 - 7.62 7.19 - 7.60 227 248 25.0 - 25.0 25.0 - 25.3 7.7 7.9 7.6 7.9 7.25 - 7.61 7.19 - 7.60 210 229 25.0 - 25.0 25.0 - 25.4 7.7 8.0 7.7 8.0 7.22 - 7.61 7.19 - 7.62 188 210 74 78 83 87 < 0.10 < 0.10 25.0 - 25.0 25.0 - 25.3 7.7 8.1 7.7 8.0 7.24 - 7.62 7.17 - 7.61 189 207 74 78 79 85 < 0.10 < 0.10

• Note: Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.

Overall temperature (oC) Pimephales promelas Ceriodaphnia dubia 25.0 84.5% 25.1 25.0 69% 25.1 24.9 34.5% Control, UV-treated 24.8 25.0 100% 297 7.36 7.40 7.8 7.7 25.1 24.9 17.25% 312 25.2 24.9 Control, Non-treated 69% 278 7.37 7.40 7.8 7.8 34.5% 25.0 25.0 Intake Intake 25.0 25.0 25.2 75 7.9 7.38 75 200 25.0 25.2 7.9 7.39 100% < 0.10 82 Ceriodaphnia dubia 25.0 25.2 199 7.9 7.39 7.37 7.39 7.37 25.1 7.9 7.37 25.2 7.8 7.8 25.0 84.5% 220 7.8 7.8 7.39 < 0.10 277 7.9 7.3 7.39 7.8 193 8.0 7.3 7.39 7.9 7.4 7.40 7.9 7.3 7.40 199 7.21 7.21 219 75 17.25% 24.9 25.0 7.8 7.3 7.42 7.21 296 7.3 7.50 7.7 7.21 312 64 88 25.1 25.1 24.8 Control, Non-treated Pimephales promelas PHYSICAL/CHEMICAL

SUMMARY

performed September 10-17, 2024. Test Sample ID Temperature (oC) Dissolved Oxygen (mg/L) Alkalinity Hardness

• Total Residual Chlorine (mg/L) pH (S.U.)

Conductance Initial Final Initial Final Initial Water Chemistry Mean Values and Ranges for UV-treated Pimephales promelas and Non-treated Ceriodaphnia dubia, Sequoyah Nuclear Plant (SQN), Effluent Outfall 101 and Intake (mg/L CaCO3) (mg/L CaCO3) 83 < 0.10 Final (µmhos/cm) 25.1 24.8 25.4 Average Minimum Maximum 25.0 24.7 25.3 7.22 7.3 7.43 238 7.7 7.8 315 25.0 25.1 84 7.3 7.41 7.21 90 7.20 7.22 7.8 7.50 7.37 237 75 82 64 < 0.10 88 64 Entered by: J. Perez Reviewed by: J. Sumner Page 8 of 93

SUMMARY

/ CONCLUSIONS Exposures to samples collected September 08 - 13, 2024 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant differences from the controls during this study period.

Entered by: J. Perez Reviewed by: J. Sumner Page 9 of 93

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas Samples used in the fathead minnow test were exposed to UV light for two minutes prior to introduction of test organisms. UV treatment is used to control interference of fish pathogens. This treatment method was approved on November 23, 2015 by the State of Tennessee in a letter from Jessica Murphy to Terry Cheek, Senior Manager of TVA Water Permits, Compliance, and Monitoring.

2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

2. Ceriodaphnia dubia None Entered by: J. Perez Reviewed by: J. Sumner Page 10 of 93

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B-2010.

4. Dissolved oxygen was measured by SM 4500-O G-2021.

5. The pH was measured by SM 4500-H+ B-2021.

6. Conductance was measured by SM 2510 B-2021.

7. Alkalinity was measured by SM 2320 B-2021.

8. Total hardness was measured by SM 2340 C-2021.

9. Total residual chlorine was measured by ORION 97-70-1977.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCl.

2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses. Entered by: J. Perez Reviewed by: J. Sumner Page 11 of 93

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Standard Methods for the Examination of Water and Wastewater, 24th Edition, 2023.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Entered by: J. Perez Reviewed by: J. Sumner Page 12 of 93

Sequoyah Nuclear Plant Biomonitoring September 10 - 17, 2024 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Entered by: J. Perez Reviewed by: J. Sumner Page 13 of 93

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, Date Sodium Hypochlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130M mg/L Quat Nalco 73551 mg/L EO/PO H-150M mg/L Quat 02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/11/2005 <0.0042 <0.0116 <0.0080 0.0199 <0.0042 0.0155 0.028 0.028 0.028 0.028 0.028 0.028 0.010 0.010 0.010 0.010 0.010 0.010 0.007 0.007 06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10/2005 0.0063 0.0043 0.0103 0.0295 0.0129 0.0184 0.037 0.037 0.037 07/17/2005 07/18/2005 07/19/2005 07/20/2005 07/21/2005 07/22/2005 0.0109 0.0150 0.0163 0.0209 0.0242 0.0238 0.026 0.026 0.026 0.026 0.026 0.054 0.009 0.009 0.009 0.009 0.009 0.018 0.014 0.014 0.036 0.036 0.036 10/30/2005 10/31/2005 11/01/2005 11/02/2005 11/03/2005 11/04/2005 0.0068 0.0112 0.0104 0.0104 0.0117 0.0165 0.035 0.036 0.036 0.035 11/14/2005 11/15/2005 11/16/2005 11/17/2005 11/18/2005 11/19/2005 0.0274 0.0256 0.0234 0.0231 0.0200 0.0116 Page 14 of 93

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, Date Sodium Hypochlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosphate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat-PF mg/L Azole H-130M mg/L Quat Nalco 73551 mg/L EO/PO H-150M mg/L Quat MSW 101 mg/L Phosphate 11/12/2006 11/13/2006 11/14/2006 11/15/2006 11/16/2006 11/17/2006 0.0055 0.0068 0.0143 0.0068 0.0267 0.0222 0.037 0.037 0.037 0.037 11/26/2006 11/27/2006 11/28/2006 11/29/2006 11/30/2006 12/01/2006 0.0188 0.0138 0.0120 0.0288 0.0376 0.0187 05/28/07 05/29/07 05/30/07 05/31/07 06/01/07 06/02/07 0.0084 0.0103 0.0164 0.0305 0.017 0.017 0.036 0.036 0.036 0.036 0.015 0.015 0.015 0.015 0.015 0.015 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 0.0241 0.0128 0.0238 0.0158 0.0162 0.0175 04/13/08 04/14/08 04/15/08 04/16/08 04/17/08 04/18/08 0.0039 0.0124 0.0229 0.0143 0.0120 0.0149 10/26/08 10/27/08 10/28/08 10/29/08 10/30/08 10/31/08 0.0260 0.0151 0.0172 0.0154 0.0086 0.017 0.018 0.041 0.041 0.041 0.041 0.030 0.030 0.030 Page 15 of 93

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, Date Sodium Hypochlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phosph ate PCL-401 mg/L Copolymer CL-363 mg/L DMAD Cuprostat -PF mg/L Azole H-130M mg/L Quat Nalco 73551 mg/L EO/PO Spectrus CT1300 mg/L Quat H-150M mg/L Quat MSW 101 mg/L Phosphate 02/08/09 02/09/09 02/10/09 02/11/09 02/12/09 02/13/09 0.0197 0.0237 0.0104 0.0155 0.0106 0.017 0.017 0.021 0.017 0.017 05/10/09 05/11/09 05/12/09 05/13/09 05/14/09 05/15/09 0.0129 0.0415 0.0053 0.0049 <0.0141 <0.0160 0.0446 0.0396 0.0396 0.0397 11/15/09 11/16/09 11/17/09 11/18/09 11/19/09 11/20/09 0.025 0.0152 0.0255 0.0306 0.0204 0.0093 05/09/10 05/10/10 05/11/10 05/12/10 05/13/10 05/14/10 0.0192 0.0055 0.0100 0.0171 0.0041 0.0099 0.039 0.039 0.039 0.039 Page 16 of 93

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, Date Sodium Hypo-chlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phos-phate PCL-401 mg/L Copoly-mer CL-363 mg/L DMAD Cuprostat -PF mg/L Azole H-130M mg/L Quat Nalco 73551 mg/L EO/PO Spectrus CT1300 mg/L Quat H-150M mg/L Quat MSW 101 mg/L Phos-phate Floguard MS6236 mg/L Phosphate 10/31/10 11/01/10 11/02/10 11/03/10 11/04/10 11/05/10 0.0122 0.0112 0.0163 0.0107 0.0132 05/01/2011 05/02/2011 05/03/2011 05/04/2011 05/05/2011 05/06/2011 0.0155 0.0179 0.0089 0.04 0.04 0.04 0.04 11/06/2011 11/07/2011 11/08/2011 11/09/2011 11/10/2011 11/11/2011 0.0168 0.0225 0.0141 0.0239 0.0242 0.0231 05/06/2012 05/07/2012 05/08/2012 05/09/2012 05/10/2012 05/11/2012 0.0145 0.0298 0.0174 0.041 0.041 0.041 08/12/2012 08/13/2012 08/14/2012 08/15/2012 08/16/2012 08/17/2012 0.0256 0.0209 0.0279 0.0076 0.0446 0.028 0.028 0.037 0.037 0.029 0.029 0.029 0.029 0.029 0.032 05/12/2013 05/13/2013 05/14/2013 05/15/2013 05/16/2013 05/17/2013 0.0099 0.0091 0.0096 0.0229 0.0063 0.039 0.039 0.064 0.064 0.064 0.032 0.032 09/15/2013 09/16/2013 09/17/2013 09/18/2013 09/19/2013 09/20/2013 0.0072 0.0107 0.0217 0.0172 0.0173 0.036 0.036 0.0379 0.0379 0.0379 0.03 0.03 0.03 0.03 0.03 0.03 Page 17 of 93

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, Date Sodium Hypo-chlorite mg/L TRC Towerbrom mg/L TRC PCL-222 mg/L Phos-phate PCL-401 mg/L Copoly-mer CL-363 mg/L DMAD Cuprostat -PF mg/L Azole H-130M mg/L Quat Nalco 73551 mg/L EO/PO Spectrus CT1300 mg/L Quat H-150M mg/L Quat MSW 101 mg/L Phos-phate Floguard MS6236 mg/L Phosphate 05/04/2014 05/05/2014 05/06/2014 05/07/2014 05/08/2014 05/09/2014 0.0118 0.0112 0.0096 0.0164 0.0235 0.0110 09/07/2014 09/08/2014 09/09/2014 09/10/2014 09/11/2014 09/12/2014 0.0070 0.0074 0.04 0.04 0.04 08/09/2015 08/10/2015 08/11/2015 08/12/2015 08/13/2015 08/14/2015 0.0195 0.0275 0.0213 0.0192 0.0182 0.03 0.03 0.03 0.03 0.03 10/18/2015 10/19/2015 10/20/2015 10/21/2015 10/22/2015 10/23/2015 0.0162 0.0125 0.0120 0.0130 0.0174 0.0156 05/15/2016 05/16/2016 05/17/2016 05/18/2016 05/19/2016 05/20/2016 0.0209 0.0210 0.0361 0.0254 0.0261 07/31/2016 08/01/2016 08/02/2016 08/03/2016 08/04/2016 08/05/2016 0.0091 0.0093 0.0209 0.03 0.03 0.03 04/30/2017 05/01/2017 05/02/2017 05/03/2017 05/04/2017 05/05/2017 0.0298 0.0218 0.0260 Page 18 of 93

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, Date Towerbrom mg/L TRC Floguard MS 6237 mg/L Phosphate/Zin c Spectrus BD1500 mg/L Quat Nalco 73551 mg/L EO/PO Spectrus CT1300 mg/L Quat Floguard MS6236 mg/L Phosphate 07/23/2017 07/24/2017 07/25/2017 07/26/2017 07/27/2017 07/28/2017 0.0124 0.0081 0.0232 0.0179 0.0296 0.04/0.01 0.04/0.01 0.04/0.01 0.03 0.03 5/13/2018 5/14/2018 5/15/2018 5/16/2018 5/17/2018 5/18/2018 0.0229 0.0159 0.0133 0.030 0.030 0.030 0.0275 0.0275 0.0275 10/07/2018 10/08/2018 10/09/2018 10/10/2018 10/11/2018 10/12/2018 0.0221 0.0098 0.0187 0.0200 0.0187 0.031 0.031 0.031 0.0332 0.0332 0.02852 0.02852 0.02852 0.02852 0.02852 04/28/2019 04/29/2019 04/30/2019 05/01/2019 05/02/2019 05/03/2019 0.0069 0.0109 0.0196 0.0341 0.0281 0.047 0.047 0.047 08/04/2016 08/05/2019 08/06/2019 08/07/2019 08/08/2019 08/09/2019 0.0227 0.0071 0.0117 0.0142 0.0078 0.047 0.047 0.047 0.0332 0.0332 0.0332 0.02852 0.02852 0.02852 0.02852 0.02852 5/3/2020 5/4/2020 5/5/2020 5/6/2020 5/7/2020 5/8/2020 0.0045 0.0055 0.0183 0.049 0.049 0.0332 0.0332 0.0332 10/4/2020 10/5/2020 10/6/2020 10/7/2020 10/8/2020 10/9/2020 <0.05

• 0.046 0.046 0.046 Page 19 of 93

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, Date Towerbrom 960 mg/L TRC Spectrus BD1500 mg/L Quat Spectrus CT1300 mg/L Quat Floguard MS6236 mg/L Phosphate 6/6/2021 6/7/2021 6/8/2021 6/9/2021 6/10/2021 6/11/2021 <0.05 0.047 0.047 0.02851 0.02841 0.02854 0.02831 0.02831 9/12/2021 9/13/2021 9/14/2021 9/15/2021 9/16/2021 9/17/2021 <0.05* 0.047 0.047 0.047 0.0329 0.0334 0.0329 0.02840 0.02836 0.02829 0.02872 0.02829 5/1/2022 5/2/2022 5/3/2022 5/4/2022 5/5/2022 5/6/2022 <0.05* 0.047 0.047 0.047 0.02846 0.02837 0.02842 0.02847 0.02841 9/11/2022 9/12/2022 9/13/2022 9/14/2022 9/15/2022 9/16/2022 <0.05* 0.046 0.046 0.046 0.0343 0.0343 0.0342 7/9/2023 7/10/2023 7/11/2023 7/12/2023 7/13/2023 7/14/2023 <0.05* 0.046 0.046 0.047 0.02821 0.02834 0.02821 9/10/2023 9/11/2023 9/12/2023 9/13/2023 9/14/2023 9/15/2023 <0.05* 0.046 0.046 0.046 0.0325 0.0326 0.0328 0.02805 0.02795 0.02801 0.02815 0.02808 6/2/2024 6/3/2024 6/4/2024 6/5/2024 6/6/2024 6/7/2024 <0.05* 0.047 0.049 0.049 0.049 0.047 0.02963 0.02850 0.02957 0.02960 0.02865 Page 20 of 93

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, Date Towerbrom 960 mg/L TRC Spectrus BD1500 mg/L Quat Spectrus CT1300 mg/L Quat Floguard MS6236 mg/L Phosphate 9/8/2024 9/9/2024 9/10/2024 9/11/2024 9/12/2024 9/13/2024 <0.05* 0.084 0.083 0.0467 0.0503 0.05049 0.05130

• = Towerbrom 960 treatments occurred on this day. TRC monitored weekly per NPDES permit requirement.

Page 21 of 93

Sequoyah Nuclear Plant Biomonitoring September 10 - 17, 2024 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Entered by: J. Perez Reviewed by: J. Sumner Page 22 of 93

Entered by: J. Perez Reviewed by: J. Sumner BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of _1_ Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client Sonit. P.O. Number: N/A Asheville, NC Other (specify): Facility Sampled: Sequoyah NP 28801 General Comments: - f'O-!.k-ed.,-1\\ \\.IJl...+ ice,. IO I Bottle Comp@ 0711 NPDES Number: TN0026450 Phone: 828-350-9364 CJr°'ol-GOD)e,,- Collected By: LJ)lfo{\\,;t,1°,,....1-er. 1 ob;c. /0oor Fax: 828-350-9368 INT Bottle Comp@ 0740 t}irl;-- ~ J '~ / ~ field Identification / Grab/Comp Collection Daterrime Container Flow Sample-Description Number & (MOD) Rain Event? Laboratory Use Volume (Mark as Appropriate) Collected ()n o~c~ 1'\\'\\lo '1-Date Time Yes If Yes, No Trace ETS Log Arrival Temp. By Time Appear-(mm/dd/yy) (ET) Inches Number (*Cl ance SQN-l 01-TOX-09082024 Comp Start ~/06/v-1 07CD End Olooo I (2.5gal) q~-ii7 ./ I -J C i)\\ ,~,() ~ 09/01/V/ "l.--/ O'\\ (A. I?., SQN-INT-TOX-09082024 Comp Start 0-1 / 01 {7.-<f 070D End 0000 I (I gal) qr..,k,!?fl / .:-I D'id\\-0'1 I~ I~ C ~ \\ ~'\\C)

• OC(/09 /l'-{

Sample Custody - Fill In From Top Down + C...~--\\ '::ll'-,'-.C. \\.,J~ "-l...'. 5 p. "'-~ ~ <le..lf.,~ Relinquished By (Signature): Date/Time Received By (Signature): ,...., ln)O~ U>__.~,,,ot-1. o\\ Date/Time ~~,;g;:-- -rJA q/7 fi1 I OC/{0 (ET) /3/( /~ "$Ot-HL ~ t LI -J Vt'( '1-7~2J/ 1 69_'/cJ (ET) 8/Z_~ Sot-l\\C.. !><f-o~ i.c; 13:iP r \\ l-- E \\°S I (ET} be. u\\le.t-~ oA.- o'\\* 'L-\\ l~\\C) ~ Page 23 of 93

Entered by: J. Perez Reviewed by: J. Sumner Whole Effluent Sample Receipt Log Page~\\

• sample temperature performed using verfied General Use Thermometer SN:

' ~ I C. 'iC.4\\SS Date Time Received Received

• sample Project Sample Sample name and description State Comments Received Received by from Temp. (

0C) number number 09-09-24 1150 J. Perez Eastman 1.9, 1.9 19961 240909.01 Eastman, River Water TN 09-09-24 1150 J. Perez Eastman 1.2, 1.2, 2.8 19961 240909.02 Eastman, Blended Effluent TN 09-09-24 1310 J. Sumner TVA Courier 1.1 19962 240909.03 TV A/ SQN Outfall 101 TN 09-09-24 1310 J. Sumner TVA Courier 1.1 19962 240909.04 TVA/ SQN Outfall 101-Intake TN Page 24 of 93

Entered by: J. Perez Reviewed by: J. Sumner BIOMONITORING CHAIN OF CUSTODY RECORD Page_l_ of 1 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 3 51 Depot Street. FedEx UPS Bus Client ~n - P.O. Number: N/A Asheville, NC Other (specify): Facility Sampled: Sequoyah NP ,* ***28801 General Comments: -F-ch,l, 0(\\ I.I.ft.-/- : (..P-IOI Bottle Comp@ 072.S NPDES Number: TN0026450 Phone:

828-35.'.0-9364

- c,--a.. ce-,cler Collected By: (pl-kl.,.._ ;t{u,...+t./\\. 1 tJLDb2 ',c. ;t,'cn.:-e-Fax: 828!.3.50-9368 INT Bottle Comp@ 07 t;(£ ~~( ' ~ Field ldenti ti cation / Grab/Comp Collection Dale/fime Container Flow pl\\>Siu:t~ f)7102-Sample Description Number& (MGD) Rain Event? Volume (Mark as Appropriate) Laboratory Use Collected Date,

• Time Yes If Yes, No Trace ETS Log Arrival Temp.

By Time Appear-

• (mm/dd/yy)

(ET) Inches Number r*c) ance SQN-101-TOX-09l02024 Comp Start .OtOD 01 /10/Zi./ End / / '-/. {f/ II 1, DIPOD I (2.5gal) 1~1./.489 ./ a'-lt,--lt11-31ti \\. \\*L i ,1..c;o 1c SQN-INT-TOX-09102024,.... Comp Statt 0100 09/ 10/v/ ~ End ov11 /~~> (JfoOD I (I gal) 1kt/-'-/K~ ./ ~~oq)l.3:} \\:-\\*L r-- \\,g::i ' \\ Sample Custody - Fill In From Top Down _. (\\l',*nl'!)'\\ S!Al-S,i..n-"<..~. :,.._KP\\.f'S.tec..e,vt<: Relinquished By (Signature): - Date/Time Received By (Signature): IN ~Ob lJ)t-)CnTll)t->, 3'\\ Date/Time ~~ ,~ q/11 /11 ID8'3g (ET) SI(~ s~t-l<-C. ~//--<1_fl I,t,*gr 1:t \\.'\\" e..c-I (ET) /4 It ~ $1t-M- ) /,i, ~3.'I 1/,2.~ (ET) r \\ h.---~ E:'5 / le.SQ (ET) ""e.u. v,W ' QC\\-l\\ - '-~ I I '-.I ~ Page 25 of 93

Entered by: J. Perez Reviewed by: J. Sumner

r':

Whole Effluent Sample Receipt Log \\, ___ -:. __ ~- --

• sample temperature performed using verfled General Use Thermometer SN:

Page fa~ I 7 2 I 6 L-( 0 5-I Date Time Received Received

• sample Project Sample Received Received by from Temo. (

0 C) number number Sample name and description State Comments 09*11*24 0950 K.Keenan Fed - Ex 1.8 19979 240911.Ol Fortron NC 09-11-24 0950 IC Keenan Fed* Ex Ll 19980 240911.02 PCS Phosphate, Inc NC 09-11-24 0950 K.Keenan Echem 1.9 19981 240911.03 Cherrv Point NC 09-11-24 0950 K. Keenan Echem 1.9 19982 240911.04 Clinton Larkins NC 09-11-24 0950 K. Keenan Echem 1.9 19983 240911.05 House of Raeford NC 09-11-24 0950 K. Keenan Echem 1.9 19984 240911.06 Lake Wacccmaw NC 09-11-24 0950 K.Keenan Echem 1.9 19985 240911.07 Mt. Olive WWTP NC 09-11-24 0950 K.Keenan Echem 1.9 19986 240911.08 Princeton WWTP NC 09-11-24 0950 K.Keenan Echem 1.9 19987 240911.09 Tabor Citv WWTP NC 09-11-24 0950 K.Keenan Echem 1.9 19988 240911. 10 Pender Countv NC 09-11-24 0950 K.Keenan Echem 1.9 19989 240911. 11 Grantsboro WTP NC 09-11-24 0950 K. Keenan Echem 1.9 19990 240911.12 KershawWTP NC 09-11-24 0950 K.Keenan Echem 1.9 19991 240911. 13 MillpondWTP NC 09-11-24 0950 K.Keenan Echem 1.9 19992 240911. 14 Vandemere WTP NC 09-11-24 0950 K. Keenan Echem 1.9 19993 240911.15 HubertWTP NC 09-11-24 0950 K.Keenan Echem 1.9 19994 240911. 16 Riverbend

• 001 NC 09-11-24 0950 K.Keenan Echem 1.9 19995 2_40911.17 Riverbend - 002 NC 09-11-24 1153 J. Sumner Eastman 1.8, 1.4 19961 240911. 18 Eastman, River Water TN 09-11-24 1153 J. Sumner Eastman 2.1, l.6, 1.4 19961 240911.19 Eastman, Blended Effluent TN 09-11-24 1158 K. Keenan Fed* Ex 1.5 19995 240911.20 Ahoskie WWTP NC 09-11-.24 1158 K.Keenan Fed* Ex 1.0 19996 240911.21 Carillon NC 09-11-24 1158 K.Keenan Fed* Ex 1.0 19997 240911.22 Farmville WWTP NC 09-11-24 1158 K. Keenan Fed* Ex 1.1 19998 240911.23 M'Kean NC 09-11-24 1158 K. Keenan Fed* Ex 4.6 19999 240911.24 Monroe WWTP NC 09-11-24 1158 K.Keenan Fed - Ex 1.4 20000 240911.25 Newton WWTP NC 09-11-24 1158 K.Keenan Fed - Ex 5.1 20001 240911.26 Yadkin Vallev WWTP NC 09-11-24 1158 K.Keenan Fed - Ex 0.9 20002 240911.27 Lincolnton WWTP NC 09-11-24 1158 K.Keenan Fed - Ex 1.6 20003 240911.28 Mocksville WTP NC 09*11-24 1158 K. Keenan Fed - Ex 1.6 20004 240911.29 Norwood WTP NC 09-11-24 1158 K.Keenan Fed - Ex 0.7 20005 240911.30 Greenville WWTP NC 09-11-24 1158 K.Keenan Fed - Ex 1.0 20006 240911.31 Rockv River WWTP NC 09-11-24 1158 K.Keenan Fed - Ex 1.6 20007 240911.32 South Fork NC 09 24 1158 K.Keenan Fed - Ex 2.6 20008 240911.33 Fairfield WTP NC 09-11-24 1158 K.Keenan Fed* Ex 0.9 20009 240911.34 Morehead City WWTP NC 09-11-24 1158 K.Keenan Fed - Ex 1.7 20010 240911.35 New Bern WWTP NC 09 24 1250 J. Sumner TVA Courier 1.1 19962 240911.36 TVA/ SQN Outfall 101 TN 09-11-24 1250 J. Sumner TVA Courier 1.4 19962 240911.37 TVA/ SQN Outfall 101 - Intake TN Page 26 of 93

Entered by: J. Perez Reviewed by: J. Sumner BIOMONITORING CHAIN OF CUSTODY RECORD Page _l_ of _1_ Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client ~;L P.O. Number: NIA Asheville, NC Other (specify): Facility Sampled: Sequoyah NP 28801 General Comments: - faded O'\\ v.,d-jl-2,, IOI Bottle Comp @ 61 /5 NPDES Number: TN0026450 Phone: 828-350-9364 _ -/wo Cro/ef"! Collected By: U5/to'r\\ Atfr..r f-o,..,, £Zeh;~ z Fax: 828-350-9368 INT Bottle Comp @ Q7 32... ~ /~ps-Fk ld Identification/ Grab/Comp Collection Datcffime Container Flow " ~ 1~11112...-- Sample Description Number & (MGD) Rain Event? ?~ Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Tr.ice ETS Log Arrival Temp. By Time Appear-(mm/dd/yy) (ET) Inches Number (OC) ancc Comp Start 0100 SQN* 10 I-TOX-09122024 OC?/tl)VI End 09/11 Iv./ r:k,00 2 (2.5 gal) 1g2., 413 o,oY .Mo~H2~ \\.\\ '\\.~ <... )\\ \\L\\\\ -l Comp Start 01 00 SQN-INT-TOX-09 I22024 C!j/11-Jitj End O'I It J /2,t.f {}oOb I (2.5 gal) qgt,l.{13 O*d-f ?il/l)"Jl3 2-7-- \\.1.o* L )\\ ,u\\ ~ Sample Custody - Fill In From Top Down

• tvs~ob~ St,A\\5 1tlTa\\~~-

$ *M-4#\\..tS (l£:{.£,'16 Relinquished By (Signatw*e): Date/Time Received By (Signature): Jt-l ~~ l.O~b,~Ot-J. ~ Date/Time ~ 1/b~ \\'\\)fl, q/rs/z1 I oz14 (ET) /jR_~ $o-.J\\L '9-1.J -2 41 le,~/ ¥ (ET) 't>t.L\\vUt.4 /3~ 2~~ 5ot-nt. 1:>e ~ v<i'd q... jJ ~ 'j ! /6',_:// (ET) (\\V-- E\\S o~- \\~*°'--\\ I \\""\\...\\ \\ (ET) Page 27 of 93

Entered by: J. Perez Reviewed by: J. Sumner Whole Effluent Sample Receipt Log Page.J.QS

• sample temperature performed using verfied General Use Thermometer SN: I ( 2 \\ G **L..\\ b :3 I Date Time Received Received
• sample Project Sample Received Received by from Temp. (°C) number number Sample name and description State Comments 09-13-24 1115 K. Keenan Fed - Ex 0.6 19979 240913.01 Fortron NC 09-13-24 1115 K. Keenan Fed-Ex 2.7 19981 240913.02 Cherrv Point NC 09-13-24 1115 K. Keenan Fed* Ex 2.7 19982 240913.03 Clinton Larkins NC 09-13-24 1115 K. Keenan Fed - Ex 2.7 19983 240913.04 House of Raeford NC 09-13-24 1115 K. Keenan Fed - Ex 2.7 19984 240913.OS lake Wacccmaw NC 09-13-24 1115 K. Keenan Fed
• Ex 2.7 19985 240913.06 Mt. Olive WWTP NC 09-13-24 lll5 K.Keenan Fed - Ex 2.7 19986 240913.07 Princeton WWTP NC 09-13-24 1115 K. Keenan Fed
• Ex 2.7 19987 240913.08 Tabor Oty WWTP NC 09-13-24 1115 K. Keenan Fed - Ex 1.1 19995 240913.09 Ahoskie WWTP NC 09-13-24 1115 K. Keenan Fed* Ex 1.2 19996 240913.IO Carillon NC 09-13-24 1115 K. Keenan Fed
• Ex 23 19997 240913. I I Farmville WWTP NC 09-13-24 1115 K. Keenan Fed-Ex 2.6 19998 240913.l2 M'Kean NC 09-13-24 1115 K.Keenan Fed* Ex 1.6 19999 240913.13 MonroeWWTP
• Ne 09-1,3-24 1115 K. Keenan Fed - Ex 3.3 20000 240913.14 NewtonWWTP NC 09-13-24 1115 K. Keenan Fed - Ex 5.6 20001 240913.15 Yadkin Valley WWTP NC 09-13-24 1115 K. Keenan Fed - Ex 1.0 20002 240913.16 Lincolnton WWTP NC 09-13-24 1115 K.Keenan Fed - Ex 2.4 20003 240913.17 Mocksville WTP NC 09-13-24 1115 K. Keenan Fed
• Ex 2.4 20004 240913.18 NorwoodWTP NC 09-13-24 1115 K. Keenan Fed - Ex 1.7 20005 240913.19 Greenville WWTP NC 09-13-24 1115 K. Keenan Fed - Ex 2.3 20006 240913.20 Rockv River WWTP NC 09-13-24 1115 K. Keenan Fed - Ex 2.4 20007 240913.21 South Fork NC 09-13-24 1125 K. Keenan Eastman 2.0, 2.6 19961 240913.22 Eastman, River Water TN 09-13-24 1125 K. Keenan Eastman 3.2. 2.0, 2.2 19961 240913.23 Eastman, Blended Effluent TN 09-13-24 0930 J. Sumner S. McNeil 0.6 19966 240913.24 North Wilksboro - Pp NC 09-13-24 0956 J. Sumner S. Smith 0.7 19963 240913.25 Graham WWTP-Pp NC 09-1:3-24 1211 J. Sumner TVA Courier 1.1, 1.3 19962 240913.26 TVA/ SQN OutfaU 101 TN 09-13-24 1211 J. Sumner TVA Courier 1.6 19962 240913.27 TVA/ SQN Outfall 101 - Intake TN Page 28 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 1 of 7 &ivlronmeoti1 TesUng Sotutlons. In<:, Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promelas Client: TVA, Sequoyah Nuclear Plant NPDES #: TN 0026450 Project#: Dilution preparation: DIiution prep(%) 17.25 34.S 69 Effluent volume (ml) 345 690 1380 Diluent volume (ml) 1655 1310 620 Total volume (ml) 2000 2000 2000 Test organism information: Organism source: In-house culture Age: < 24-hours old Spawn date: 11~-o*\\:,A 84.5 100 1690 2000 310 0 2000 2000 County: Hamilton Outfall #: 101 Treatment: UV (Each concentration was UV-treated for 2-minutes to remove pathogenic interferences.) Sample was not aerated or treated unless otherwise noted on this form. Sample was warmed to 25.0 +/- 1.o*c In a warm water bath and then diluted to the test concentrations with moderately hard synthetic water (MHSW). Test information: Randomizing template: Gue,.j Incubator number 7 i;:_ and shelf location: Artemio CHM number: CHM1294 ~-()"I* '\\."\\ ~~S TO Drying information for weight determinatlan: Hatch dates and times: oo.,,o* "" o~ oS Date/ Time in oven: ()C\\*11*'\\...\\ N J\\C

• initial oven temperature:

, _11

• L Transfer vessel information:

pH (S.U.)= 8 -l!) Date/ Time out of oven: O"\\* I( *~..\\ t>W>C. Temperature (*C) = '\\.'\\. 0 * <-

• Final oven temperature:

lat\\" (.. Average transfer volume (ml): <0.25 ml Total drying time: '"\\.."h~o-.>.A.~

• Go*c Oven, Thermometer SN: 14-98585 Daily feeding and renewal information:

Day Date Morning feeding Afternoon feeding Test initiation, renewal, Sample M HSW or termination numbers used batch used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 0 09-10-24 ~00 \\{ l~~C) \\A 0"1-t°'l \\.{ 1.'1~~- 0~ \\.'-l O'\\o'i.o..f o~<<*'\\-\\ -/4. 1 09-11-24 Iv - }( ..\\, L j C<::,,"lb \\lOb I,,{ o*w1-2 09-1.2-24 t,~QI:) \\I\\ l.1.0Q IA I, (7'\\'!)' I~ "'l.-1~\\\\_ ?,I.. "t~O'\\\\\\. ~""'\\ O'H>~*\\,( I) 3 09-13-24 ~~\\)() ~ \\\\~b '\\i <n~ \\A j_ l 4 09-14-24 11 ' \\'\\(Al~ '\\lo o O\\* O't*1.-l& O"-~b \\I \\"\\.00 IA Od~o IA "'\\.~oc;,~.,.., s 09-15-24 01ooo "X1 , "1.soo h Clef"'-" 0 ' \\ \\ J 11 6 \\t\\. V ~ 4 J, J, 09-16-24 C)~OO \\\\ \\:> l\\ O"\\~I;) 7 09-17-24 I..._ 01o c:. 1- "K Chemical analyses: Parameter Reporting Limit M ethod number M eter Serial number pH 0.1S.U. SM 4500-H+ 8-2021 Accumet AR20 93312452 Dissolved Oxygen (D.O.) 1.0mg/L SM 4500-d G-2021 YSI Model 52CE 08A100271 Conductivity 14.9 µmhos/cm SM 2510 B-2021 Accumet AR20 93312452 Alkalinity 5.0 mg CaCOJ/l SM 2320 B-2021 Accumet AR20 93312452 Hardness S.O mg CaCOi/l SM 2340 C-2021 Not applicable Not applicable Chlorine, Total Residual 0.1 mg/l ORION 97-70-1977 Accumet A82SO 92349123 Temperature 0.1 *c SM 25508-2010 Digital Thermometer t~obb'-\\lotS Control information: \\.l \\)- "'"~"~ e 't> Acceptance criteria Summary of test endpoints: % Mortality:

01.

~20% 7-day LCso (%) '") 100 7. Average weight per initial larvae: 0-<i~Z... NOEC (%)

4007, Average weight per surviving larvae:

0 -4~,._ > 0.25mg/larvae LOEC (%) )tM7. ChV (%) ..., /(X) 1, ICzs (%) ). JO() 1. SOP AT20-Revision 6-Exhibit AT20.3 Page 29 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 2 of 7 Envlronmenc1I Teuln9 Solutions, Inc.. Species: Pimephales promelas Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 09-10-24 Survival and Growth Data Day CONTROL 17.25% 34.5% A B C D E F G H I J 0 10 10 10 10 10 10 10 10 10 10 1 ,~ l \\) ' \\) /0 JO H) , 0 ll) IC:, 10 2 ,~ IC l \\) I C) 10 l~ 10 I () IO , 0 3 /() 10 10 I~ 10 I t) I 0 ( () IQ I () 4 lb It) 10 lo ID 10 It) It> ll) 10 5 '() IO 10 10 It) ID lb 10 Lb ID 6 l~ I t, IQ l~ l t) I\\) ID ID 10 ID 7 lO l~ IQ,o f \\') 10 10 (Q (Q 10

• A= Pan weight (mg)

Tray color code: fwfl !YJ;~e. '~~ .;x_o.. 'o°' Analyst: f..1 4lo ,~~? (>.,'\\. 'o~ 1\\1\\

x\\

0 Date: f:l?. 7 ~ 1,.1,( .._rv* v\\. iX \\ ~ - . \\; 1,'1/2 *

• 0' ~ -

{") : "\\ \\

• B = Pan+ Larvae weight (mg}

Analyst: 1-1.ul.. '"*~' -n,,<T\\ Date: h:~. l'\\.*"L-\\ 1.1..&<., '\\1,.l"l "\\1...c;& 't"!t.lS"\\ n,"'1. ~1,.l\\ 'l,-... ~s C = Larvae weight (mg)= B -A i-~,.1.'l. I.So& fl\\.\\l. '\\,loo o\\.Cf1 i,-t\\{ i,lo4:. ~-10 l\\,l.S Analyst: ~ Weight per initial number of larvae (mg) = C / Initial number of larvae N cif ~ ~ 0 ~ \\It., f\\,Q q, °' ~ ~ C\\; 'Iii ' Analyst: ~ ()' 0 'O' o* o* o* o* ()' ,;,::i o* Average weight per Initial Percent number of larvae (mg) reduction o, &?>1.. O.&ct~ -,.... 1. o.art~ from control(%)

• Weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520.

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk= sick, sm = unusually small, lg= unusually large, d&r = decanted and returned, w = wounded. I

Comments, K

L 10 10 I 0 ID ' 0 IO , 0 <O 10 JO ' () io I 0 lb 10 10 ~o I).,\\. 0* v..* '\\; l.'!i.o'-l n.8'-1 ° "l.'1 8.!.~ ,¢, ti,. ti o* -,.~ 7. SOP AT20-Revision 6-Exhibit AT20.3 Page 30 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 3 of 7 Envl,onmtnt-.lTHtlng Sclutlons. Inc, Species: Pimephales promelas Client: TVA/ Sequoyah Nuclear Plant. Outfall 101 Date: 09-10-24 Survival and Growth Data Day 69% 84.5% 100% M N 0 p Q R s T u V w X 0 10 10 10 10 10 10 10 10 10 10 10 10 1 (\\) /\\) I 0 IO /O to '0 '() I l) ' l) /0 {0 2 I D I{) (~ t () c D 10 IQ 10 lQ ID / 0,o 3 I C I 't) It) I r;::, ( C) , D ( \\) 'C I\\) IQ ( ti I(,) 4 ID l t) lb I D I'D IQ IQ ID ID I D IQ to 5 10 I \\I I\\) I \\) It) JD 10 10 IO 10 10 '0 6 \\~ l () 10 \\ C \\ ~ H) I() IC '° /Q IO,o 7 ID ro I \\\\ IC) JO /0 10 I 0 10 I 0 I 0 10 'A= Pan weight (mg) Tray color code: 11<\\Ji!!,a','le I\\\\. 'h"' 1£i Analyst: lfvi \\,t>-, o'v 0\\, C>. - '\\,Qi o._'V "1/2a -:x,"1 '-a'1/2 C2~"2.1-'2,U: I\\,,. ~* v Date: 0* "). ~- ~ - ~ * ,I\\,* '1,- ~. {11' \\ \\ '\\, \\\\)(.

• e = Pan + Larvae weight (mg) lC>,°tb Analyst:

~ 2.,.1.0 11-1>* 'L"\\ '11.18 1:l-~" "lt\\.C>i,1.c.f ~-lo'L ,. '1.,'i,. u.o\\\\ l'i*l-, 1.1.,,S Date: Qt-1~-.~ C = Larvae weight (mg)= B--A s.1.16t,.~i &,o'\\,.~'l. '\\.ot. &'-'!I°' &.~i.\\ &, 1.§ '\\,00 8,1~ i..&o 8, '2-- Analyst: It\\ I > Weight per initial number of larvae (mg) = C / Initial number of larvae °' ~ 8- "~ ~ ~ ~ 'O'V,,~ ,~ (lo ~o ,v I_; ~ ~ ~ i\\l ~* 0,.. 6',; Analyst: Q ~- o* o' 0 b" ~- t;;,' o* 0 I) Average weight per initial Percent number of larvae (mg) reduction 0.111 ,. a 1. o.i~I .,.cii7. o.a,1 t.q 7. from control (%)

• Weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520.

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg= unusually large, d&r = decanted and returned, w = wounded. I Comments: SOP AT20-Revision 6-Exhibit AT20.3 Page 31 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 4 of7 Envlronment1ilTestrng Solu1fon'- Inc. Species: Pimephales promelas Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 09-10-24 Survival and Growth Data Day 100% Intake Control, Non-treated y z AA BB cc DD EE FF 0 10 10 10 10 10 10 10 10 1 to I 0 It) '{) 't) IQ IC 10 2 ({) , 0 I {) I () I 0 I b I b

11) 3

/0 IQ I 0 , 0 to I D ID ID 4 / t) 10 10 ID I\\) 10 10 10 5 lC) 10 ID IQ 10 I{) lQ ID 6 \\ C) IC) 10 jC) 10 It) 10 ID 7 10 10 I 0 10 I~ 10 I 0 I()

• A = Pan weight (mg)

Tray color co,~f ::G,cq,142~ S ~ '1/2~ \\..:,._ "ofb ~ Analyst: ~ cx,O r,P 0o'l-i D11te: Q.1J.~1..:1~Vf' \\. ~- 0* 1v'

j._,

~- M'

• o*

I\\. ,o*

• B =Pan+ larvae weight (mg)

Analyst: )1 ).\\.-\\\\ 1.\\-~C.. ~,.l.\\o Date: 0, 01"--"' 1,b.~--\\ ll-~"'- ~-~I\\ *n,.t.() 1,l.1~ C = Larvae weight (mg) = e -A

a. 'l~ 1.0 l

,§. lie, ,. I.lo s.~,. &,~C\\ &.11 q.olo Analyst: ~ Weight per initial number of larvae (mg) = C / Initial number of larvae I\\'\\ "'"" "~ ~ ~,y ~ ~~ ~ q, ~ ' ~ Analyst: d4 0 'u o* o* rs rJ o* t)' Average weight per initial Percent number of larvae (mg) reduction a,,1'i from control (..\\( 1. 0.111 NA (%)

• weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520.

Comment codes: c = clear, d = dead, fg = fungus, k-= killed, m = missing, sk = sick, sm = unusually small, lg= unusually large, d&r = decanted and returned, w = wounded. I Comments: SOP AT20*Revision 6-Exhibit AT20.3 Page 32 of 93

Entered by: J. Perez Reviewed by: J. Sumner I

• Environmental Testing Solutions, Inc.

toAnA,rMlan I") A* plim* ln.ltl-'-""'"'b.rof Fm.,"~... or lN'o'M cc JO JO

Control, DD 10 JO Non-tr rilled EE JO 10 FF 10 IO A

10 10 Control1 JO 10 UV*treate.d C JO 10 D 10 10 E 10 10 17.;!57' F JO 10 G 10 10 H 10 10 I 10 10 34.5% j JO 10 K 10 10 L 10 10 M 10 10 69'1 N 10 10 0 10 JO p 10 IO Q 10 10 84.51' R 10 10 s 10 10 T 10 10 u w 10 100% V 10 10 w 10 10 X 10 10 y 10 10 100% Intake 2 10 10 AA JO 10 10 10 M!!!1.l2l; Du.nnett'1 MSO \\IAlue: 0,1080 PMSD: 13.0 1nt1lle* ounned '1 MSO \\l~lue: 0.097_5 PMSD: lU ~ ;F,-,. wet,cht l"'1l

14.90 10.90 H.D 1.4.17 12.73

• 12.16 1.4A9 l4.69 13.73 13.91 14,63 13.77 13~41 15.10 13.80 14.31 12.91 14.86 13.69 15.02 11.06 13.2.9 15.28 12.78 13.92 14.JO 12,47

-i4.63 13.18 14.34 13.14 12.68 MSO

• PMSO
• TVA/ Sequoyah Nuclear Plant, Outfall 101 September 10-17, 2024 Plmephales pramelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyse.s Not for Ct>mpliance AsH.Mtne.nt; ln1ern,1l Li1boratary QC I s,-,,*~

!MvNwa,l[l:!1(md W*fcf'lt / Swrvi~r ..... " w.i,.,.. 1 Coeffidwit of ~-,I.UC.ft w.lrht IFT'ld ~um IN, of '-tftt (ml) ~~n,'IVffl.'o.rot ,....... - 1p,1,.., _..,... 23.H 8.42 0.842 .l.9.49 8.59 0.8S9 0.871 3.1 22.60 8.77 0.877 23.23 9.06 0.906 21.06 833 0.833 19.98 1.n 0.722 0832 9.8 23.07 858 0.858 23.85 9.16 0,916 23".33 9,60 0.960 22.98 9,07 0.907 0.89'1 5,7 23.07 KA4 0,844 22A2 8.65 0.865 22.11 8.70 0,870 2A.35 9.25 0.925 0.893 4.l 23.04 9,24 0,924 22.84 8.S3' 0.853 21.60 8.69 0.269 23.24 8,38 0,838 0.817 6.1 2L78 8.09 0.809 2254 752 0,752 20.08 9.02 0.902 21.68 8.39 0.839 0.848 4.4 23.62 8.34 0.834 20.96 8,18 0.818 22:92 9.00 0,900 23.04 8.74 0.874 0.817 12.0 19.27 6,80 0.680 22.75 8.12 0.812 21.41 8,23 0.823 21.lS 7.01 0,701 0.779 1.6 21.40 8.26 0.1126 20.34 7.66 0.766 Mlrlfrn\\4m Slgoft'ion\\ Otfference Percent Minimum Sla;nifrcant Diff~rence PtoJMt nombert 19962 W.irht / tnltt.r A1o1mlN,r Mtwi, *wM.,.i MMA-1,tll/ CO*ffkhnt of Pe,~, fffo.1'1Son from oflMYH(,n.r) (oi) lnhl*l l'liH?llMtt(lf !Mrlatloa...,.. w.1111t Ulf\\1'01 It.) ,.,...,..(n,s] .,..l,,ltW __ ,..,,,,,u,.) ,w, 0.842 0.859 100.0 0.171 l-1 Not appllable 0.877 0.906 D.833 0.122 100.0 0.u2 9.1 Not applicable 0.858 0.916 0.960 0.907 lllO.O 0,8~ S.7 -7.4 0.844 0.865 0.870 0.925 100.0 0.893 4.1 -7.3 0.924 0.853 0.869 0,838 0.809 100.0 0.817 6.1 1.8 0.752 0.902 o.n9 100.0 0."8 4.4

• 1.9 0.834 0,818 0.900 0.874 0.680 100.0 0.817 12.0 1,9 0.812 0.823 0.701 100,0 0.779 7.6 6.4 0.826 0,766 PMSO fs II Oleilsure ot testprecfsK1n~ The fMSO rs the mfrilmum pcorcent dlfferenoe between the conl'tol and rre.~tmehl th*t '-'n be dedaced 5~lliltcaUy slgnlUc~nt Ina whole ~fOUent to)(ldty test, Lower PMSO bouod determined by USEJJA (10th pem.;nl/Je) "' 12%.

U,:,per PMSO bound determfned bV USEPA (90th puct.ntile) ~ 30%. Low*, ~nd upp~rPMSO bounds*weni determined from the 10th ~nd 90th pemmli1", r"s.pecOve/v, o, PM50 dat.a from EPA's w*er ln~labota\\oryVarfllblllty Study(USEPA, lOOta; USEPA, 200\\b~, Lls(PA.. 2001a, 200lbc final Reporc lnte,l.1boracory VirfabilityStudyof EPA Shor1-t1:,mChronic o1nd Acute Whole Etlluent Toxicity Ten Me,thods, Votuml!!s l aM 2*Appendlx. EPA*82l*S.OU)U4 ind EPA-82.l*B*Ol*OOS. US Envrronm,rn~I Protec1ion Aaency, Cfnctnnati, OH. .r.:::... - "I '="°~ -i" Page 33 of 93

Entered by: J. Perez Reviewed by: J. Sumner Stan Date* 9/10/2024 TVA/ Sequoyah Nuclear Plant, Outfall 101 September 10-17, 2024 Statistical Analyses Larval Ash Growth and Sur,ival Tett-7 °'IY Gro'WIII TesllD! PpFRCR Sample ID* TVA/SON Ou~all 101 End Dale: 9/17/2024 Lab 10: ETS-Envk_ Testing Sol. Sample Type, DMR-Dlscharge Moni\\onng Report Sample Date: Seplem ber 2024 Pro1ocol; FVVCHR-EPA-821-R*02-013 Test Species: PP-Pimephales promela* Comments: Cone-% t 2 Non.Control 0.8420 0,8590 0,8770 0,9060 UV-Conirol 0,8330 0.7220 0.8580 0.9160 17.25 0.9600 0.9070 0.8440 0.8650 34.5 0.8700 0.9250 0.9240 0.8530 69 0.8690 0.8380 O.S090 0.7520 84.5 0.9020 0.8390 0.8340 0.8180 100 0.9000 0.8740 0,6800 0.8120 Intake 0.8230 0.7010 0.8260 0.7660 Trana form, untransformed Cone-% MHn II-Mean 1111111.n Min Max CV% N I-Stat Non.Control 0.8710 1.0466 0.8710 0,8420 09060 3,141 UV.Control 0.8323 1.0000 0,8323 0,7220 0.9180 9.770 4 17.25 0,8940 1.0742 0,8940 0,8440 0.9600 5.726 4 -1,378 34,5 0.8930 1.0730 0.8930 0.8530 0,9250 4,147 4 -1.356 69 0.8170 0.9817 0.8170 0.7520 0,8690 6.093 4 0.340 84.5 0.84B3 1.0192_ 0.8483 0.8180 0.9020 4,354 4 -0.357 100 0,8165 0.9811 0.8165 0 6800 0.9000 12.027 4 0.352 lnlake 0.7790 0.9360 0.7790 07010 0.8260 7.557 4 AUXIiiary Tuts Stattstle Shapiro-VI/ilk's Test Indicates normal dlslrlbuUon (p > 0.01) 0.95905 Bartlelfs Testlndlcates equal variances (p = 0,48) 4-48146 The control means are not signlflcanlly dllleront (p = 0.40) 0.90339 HypothHl9 THt (1-blll, 0.06) NOEC LOEC ChV TU IIIISOU MSDp Dunnell'& Test 100 >100 1 0,10798 0.12975 Treatments vs UV.Control LlntlT lntwrpolatlbn 1200 RHaml)leS} l>otnl tC05 IC10 IC15 IC20 IC25 IC40 IC50 ~ 87,572 >100 >100 >100 >100 >100 >100 SD 15%CUExpl Skew 1.0 0.9 0.8 0.7 0.6 C, Oli i o_* .. 0.3 CZ: 0,2 0.1 0.0 -0.1 -0.2 0 t'l'*lled Crlllcal 1111S0 2,410 0,1080 2.410 0.1080 2.410 01080 2,410 0.1080 2.410 0.1080 Ctlllcal 0,884 15,0863 2.44691 1111S8 MSE 0.00505 0.00402 50 100 Dose% Dose-Response Plot 1.2 T""--------------------, ~ ..... 0,4 0.2 0 'l! ls 'i II z ~ ls ~ ~ "l "' ;t ~ j/ ~ 1-tail, 0.05 lel1!1 or significance laolOnle MHn II-Mean 0.8731 1,0000 0 8731 1,0000 0.8731 LOOOO 0.8326 0.9537 08326 0,9537 0.8165 0.9352 Skew Kurt -0,6413 0.39349 14'rob df 0.32415 5, 18 150 Page 34 of 93

Entered by: J. Perez Reviewed by: J. Sumner Environmental Teulng Soluctont, Inc.. Start Date: TV A / Sequoyah Nuclear Plant, Outfall 101 - Intake September 10-17, 2024 Statistical Analyses Larval Ash G-owth and Slrnflval Test-7 Day G-owth Test ID: PpFRCR Sample ID: TVA/ SQN Intake End Date: 9/10/2024 9/17/2024 Lab ID: ETS-Envir. Testing Sol. Sample Type: DIIIR-Discharge llllonitoring Report Sample Date: September 2024 Comments: Protocol: FWCHR-EPA-821 -R-02-013 Test Species: PP-Pim ephales promelas Cone-% Non-Control UV-Control 17.25 34.5 69 84.5 100 Intake 1 0,8420 0.8330 0.9600 0,8700 0.8690 0,9020 0.9000 0.8230 2 0.8590 0.7220 0.9070 0.9250 0.8380 0.8390 0.8740 0.7010 3 4 0.8770 0.9060 0.8580 0.9160 0.8440 0.8650 0.9240 0.8530 0.8090 0,7520 0.8340 0.8180 0.6800 0,8120 0.8260 0.7660 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max Non-Control 0.8710 1.0466 0.8710 0.8420 0.9060 UV-Control 0.8323 1.0000 0.8323 0.7220 0.9160 17.25 0.8940 1.0742 0.8940 0.8440 0.9600 34.5 0.8930 1.0730 0.8930 0.8530 0.9250 69 0.8170 0.9817 0.8170 0.7520 0.8690 84.5 0.8483 1.0192 0.8483 0.8180 0.9020 100 0.8165 0.9811 0.8165 0.6800 0.9000 Intake 0,7790 0.9360 0.7790 0.7010 0.8260 Auxiliary Tests Shapiro-VI/ilk's Test indicates normal distribution (p > 0.01) F-Testindicates equal variances (p = 0.61) The control means are not significantly different (p = 0.40) -sis Test (1-tall, 0,05) Homoscedastic tTest indicates no significant differences Treatments vs UV-Control CV% 3.141 9,770 5.728 4.147 6,093 4.354 12.027 7,557 N 4 4 4 4 4 4 4 4 Statistic 0.93692 1.90736 0.90339 MSDu 0.09753 Dose-Response Plot 1.2..--- --- T r -_t T I I ~ 0.8 _____ 1 ___________________ r ______ ~ ----*P ~ 0.6 ~ r-- OA 0.2 0 g g I() I() ffi I() §! .2l N ~

i a

C: ~ 0 ~ q ~ ~ 1-Talled t-Stat Critical MSD 1.061 1.943 0.0975 Critical 0.749 47.4683 2.44691 MSDp MSB o_ 11719 0.00567 1-tail, 0.05 level of significance MSE 0.00504 Skew K...-t -0.6868 -0.432 f4)rob df 0.32955

1. 6 Page 35 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 5 of 7 Envlranmenul ifftlng Solutions, Inc.. Species: Pimephales promelas Date: 09-10-24 Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Daily Chemistry: Temperatures performed at the time oftest initiation, renewal or termination by the analyst identified in the Dally Renewal Information table located on Page 1. Alkalin\\ty, hardness and chlorine (total residual) performed by the analyst identified on the bench sheet speclfic for each analysis and transc.ribed to this bench sheet. Day (Analyst Identified for each day, performed pH, 0.0. and conductivity measurements only.) 0 1 2 Analyst r1-

1. v,tl.,

g.,, t1./ /l.A-7 "-- /J:,"7 J/1.. IA, Concentration Parameter pH(S.U.) ,.J.'i~ --; 2. "1

7. 3'2-7,~

?, 1/2-l.o.8"'::) Dis.solved oxygen (mg/l) IJR> '.'l 't-9 ~-4 ,q~ "15 UV-Treated Conductivity (µmhos/cm) jnn 3-Z-/ J;J

CONTROL, Alkalinity (mg CaC0JL) l.-\\

- r---. ~ MHSW ~ Hardness (mg CaCOJL) 8'1 --- ' 1111-ftWI 11. "6 Temperature ('C) "l.*V\\ '"1,fi. 'L "\\~-i ., <-. l '-1\\. e '5 '!:. pH (S.U.) '..J-.Li5 1.1--6 ?. ~> ?.2-9 7.<f/ IP. th\\f 17.2S% Dlssolved oxygen (mg/l) ':\\Q> ' ~.) -~.'\\- 'l"' 9 ~ CiJ." Conductivity (µmhos/cm) "2.-81~ 3o5 "$'0'-1 Temperature (*C) ~ . 1) -i.\\. 0 ""* '\\ "\\\\. l '\\.'\\." '\\.Ci. 1) pH (S.U.) 4.lr? ?, 2,.6

7. 3</-

7, J.~ 7, 11-0 1o.e,s 34.5% Dluolved oxygen (mg/L) ':\\~ -1: '), .1- ~-~ /4..8 CJ.~ Conductivity (µmhos/cm) 2v

• 2,a2.

-~~ Temperature (*C) ~-0 "\\S.1) ~ .f) "l...\\." "'\\."\\..j ~-; pH(S.U.) I.I I 7, 7 c; 7,.33* 7.2~ 7, ~*7 !d.,8(} 69% Dissolved oxygen (mg/L) ~-'i ~-~ ' -f.d ~ -lo '1/2', C: ~ _111.S Conductivity (µmhos/cm) ,._'JO 21./-2. -,~~ w. - 1 Temperature (*C) '\\S -0 "\\.~-> '"\\.S

• t)

~ -'\\.... "\\S,O ~- <> pH (S.U.) ' +.4 7.L7

7. 13 7.12...

?.39 loft2 84.5% Dissolved oxygen (mg/L)

• l'.J--:i

~-1 '.J,8 '+,la ,...J ( l.i.'-\\ Conductivity (µmhos/cm) '217 27-~ ~

• --r Temperature (*C)

'lS-0 ~ -0 '"\\.~. 0 "\\.~- f' ~ '\\S.b pH(S.U.) ~,1/42.

7. J.,.~

7.14

7. 7.--7

7. ?~

{b,09 Dissolved oxygen (mg/L) \\(), 0 't,1-- '}.'I '.:\\.b ~o eo:o Conductivity (µmhos/cm) l 'l c. l ?n'2-L 'T'X ~ O"'I \\~I,\\{ 100% Alkalinity (mg CaCOJL) -,g ,~ Hardness (mg CaC0,/L) g?_) a~ Chlorine (mg/L) <.o. >0 <. 0.,o Temperature (*C) 15,0 "\\.'l. 'i 1.S-D "'\\.'1. f \\ S,o "\\.~C\\ pH(S.U.) /l-4~ ?,1-'?,

7. 3r:;

'7 2.6 7.3~ V.~2-Dissolved oxygen (mg/L) ~,

• • n

'lR 'l. '6 ~..o I /l, 2... 100% Conductivity (µmhos/cm) !Ci '2. 'lo { 19-'f-Intake Alkallnity (mg CaC0Jl) , k' ,--1 Hardness (mg CaC0JL) R~ 8\\ Chlorine (mg/L) ,<. 0.1 D <o.,o Temperature ('C) ~.O '\\.'(. '\\ '\\S.l) '\\.'{.Cl\\ 1.~.o "'~'-t-°' Initial Final Initial Final Initial Final SOP AT20-Revision 6-Exhibit AT20.3 Page 36 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 6 of7 Erw1tonml!nt1ITettlng Solutions. Inc. Species: Pimephales promelas Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 09-10-24 Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 3 4 5 6 Analyst "(V ~/;~ Q6(... 6'5v 0$v &-1 JJ,,-,, /y? Concentration Parameter L... ~...... -~ pH(S.U.) 't.2Pl 1,~o -:J.* ~.~* 1,d.0

J.-4(#

?, _c;*7 7,6*? 7./7 Dissolved oxyien (ms/L) \\.in l."'.1- -:V~,.'I 1-.v 1 Pi ,.le, UV-Treated ConductMty (µmhos/cm) ~09 !n, I., ~,. >Z I

CONTROL, MHSW Alkalinity (m1 C.CO,/L)

--~ A Hudneu (mg CaCO,/L) ~ ~ .. ~-- Temp.,ature ('C) ""\\ "1. fr "'\\.<;,.\\ -ul* 1 ~I\\ ~.A ti. I "\\.'\\, 1 ""'LS.-,.. pH(S.U.) /l.i+ 1,~0 1,3l 1.,.,. >3/4, 7,.s.:s "?,66 7.17 17.25% Dluolved O"'flOO (ms/L) "1--.,1,, l,°t is -:J.,/:\\ '"'\\.,_ ,.7 '. L, Conductivity (µmhos/cm) -~~o "'I~ ~'M 291 Temperature re) "\\.-\\.~ '\\.~. f '-"* '\\ "'-$. l ""\\..'\\,i "\\.'\\. '\\ '"\\),\\.., ~~-l. pH(S.U.) 't.1.A- -=I,'"' -:J,31, 1-.\\i +.~ 7,54-7, d ti 7,/6 DlsJolved o*vcen (ms/L) I +.i ,t' 'i.o ~3 'l _., -v\\ 7,\\ . """\\... 34.5% "L~l ,~~1 ,;\\ g.:> 2 7'7 I Conductivity (µmhos/cm) Temperature re) "\\.'\\. 'l "'\\.'\\. ' \\.-l-'\\ "\\.t;,.,_ '\\.'\\. q '\\.~.l.,. ... "'.E '\\.S. 'L pH (S.U.) '\\-.2.4 ":l,,q 1-,31#- -:J-.;;)3 +-'-+~ 7,5'/ 7J,<vcen (ms/L) ' a 1-.. f" C:,.o -:i.a ~-0 7.--\\ 7-\\ ,.~ Conductivity (µmho,/cm) 2l4 ld2-Ct ~\\ 2?& Te.mpuature (*C) ~ -'t> '\\St. 0 '\\..-l.~ "\\.S-'L ..,_.v, \\.").'L '\\>\\,'I '\\.~.o pH (S.U.) , 1.2..?i

f,,q

'"l,~~ I l-. 2-+ J.:43 7.S( 7.6 z.. 7. / 2-Dlnofved O"'flen (ms/L) l)Q +,1- ~-~"' 'l, <j.o 7-""'\\ -,. ~ 84.5% Conductivity (µmho,/cm) 21~ ~11- ~.:i,

• n.l Temperature ("C)

"\\.\\. 0 'U,O ""\\..-t,'\\ ,..~_,_ '"'\\."\\.'\\ "\\.~.,_ 1$,.Q "\\.S.1-pH(S.U.) r.l~ ~.ao

J,~;

=l.15

J.'1/43

?sz.. 7,65 7,13 Dlnolved O"'flen (ms/L) , ~

J-~

<a.o

J.,(

i..o \\ * > \\..9 ""'\\,1-Conductivity (µmhos/cm) ... ~ l'iS At>'+ J'i~ 100% Alkalinity (me C.CO,/L) ,'-l Hardness (m1 CocO,/L) Ir"" Chlorine (ms/L) <o,LO Temper*turc ('C) -u.c "\\l;.,Q ,~.o I.S* 1... ~S, o "\\.<;. 0 '"'\\.S-0 1.c;,'\\- pH(S.U.) r1/4.2.0

J. Ii

'l.a....

J.,;

1.3'l 7.St ?, 1,/. 7.{?_ Dissolved O"'fl<n (ms/L) '¥.i 4* 1°" 'f,O 'f.4 '1,.:> ,.'\\ 7.!

• . 'L Conductivity (µmhos/cm)

IA'1 10/ I ,qs / 92.- 100% Alkalinity (rn1 CICO,/L) '"\\~ Intake Hardneu (me C, CO,/L) 8\\ Chlorine (m,/L) <0.1() Temperature (*c) '\\S.O "\\.'i. 0 :i *O -U:., (') "\\.$... t:) "Lt,~ t~-0 v... 1-Initial Final Initial Final Initial Final Initial Final SOP AT20-Revision 6-Exhibit AT20.3 Page 37 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 7 of 7 Envtronment*I Ttstlng Solutlon1, Inc. Species: Pimephales promelas Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 09-10-24 Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 0 1 2 Analyst V\\ I{,.,,. .A /i;<,? V" ~ vl, ~ ~- Concentration Parameter pH (S.U.) ,.,~ 7,"?0 7.4-9 7.Z-7 '-l-~ IO.~'? Dissolved oxygen (mg/L) 7, ' ~.10 4.?J 4:1-q _~ ld.z.. Non-treated Conductivity (µmhos/cm) "\\.'\\ ~ ~>0 ~lt'J

CONTROL, Alkalinity (mg CaCO,/L) l... '-l

~'-\\ MHSW r-- Hardness (mg CaCOJL) 8"\\ t'\\ Temperature (0C) '\\.'-\\.,'\\ '"'*" ,.'\\, 1 "\\..~. \\ 1."\\. '\\ ~ Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 3 4 5 6 Analyst i,A-6St.., ~\\,. e6v P-61.- ~ 6..-, ~~ Concentration Parameter I pH (S.U,) Ir.} tP

J ;;i I

J..43 1,11

+.\\(, l 7,55

7. ~ (

7./~ Dlssolv*d OJl'/l*n (mr/L) r l-.'l 1-~ l,t

i.~

18 ,-~ ,.lo \\,\\..... Non-treated Conductlvlly (µmhos/cm) lnt 31\\D ~1 3to

CONTROL, Alkolinlly (me C.CO,/L) 1

-.st< \\.A .odJI MHSW Hardness (me C.CO,/L) ~* .e1 ~c Temprr1tur* ('C) ,'-\\. \\ "L<; -0 'l.'-\\., '\\.I;.."\\_ \\.-.\\. 7 "\\.~ -\\ "\\."- f \\.S,1-SOP AT20-Revision 6-Exhibit AT20.3 Page 38 of 93

Entered by: J. Perez Reviewed by: J. Sumner Environmental Testing Solutions, Inc. Concentration Parameter DH {SU) 00 (m2/Ll

Control, Conductlvitv /umhos/cml Non-treated Alkalinity (mg/L caco,)

Hardness (mg/L Caco,) Temperature (°C) pH (SU) DO (mKIL)

Control, Conductivity (11mhos/cm)

UV-treated Alkalinity (mg/L caco,J Hardness (mg/L Caco,) Temperature ("Cl pH (SU) 00 (mKILl 17.25% Conductivity (umhos/cm) Temoerature {"C) pH (SU) 34.5% DO (mg/L) Conductivity (umhos/cm) Temoerature (°C) oH (SU) 69" DO (m2/l) Conductivity (l!mhos/cm) Temperature (°C) pH (SU) DO (mg/L) 84.5% Conductivity (11mhos/cm) Temperature r"CI pH (SU) DO (mE/L) Conductivity (11mhos/cml 100% Alkalinity (mg/L.CaCO,) Hardness (mg/L caco,)

• Total Residual Chlorine (m2/l}

Temoerature { 00 pH(SU) DO (mg/L) Conductivitv (umhos/cm) 100% Intake Alkalinity (mg/l CaCO.J Hardness (mg/L eaco,)

• Total Residua! Chlorine (mE/L)

Temperature {°CI TVA/ Sequoyah Nuclear Plant, Outfall 101, UV-treated September 10-17, 2024 Dayo Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Dav 1 Dav 2 Day3 Oay4 Initial Final Initial Final Initial Final Initial Final Initial 7.75 7.30 7.49 7.27 7.32 6.83 7.62 7.21 7.43 7.7 7.6 7.8 7.7 7.8 6.2 7.7 7.8 7.7 298 330 318 304 316 64 64 64 87 89 87 24.9 24.9 2.4.7 25.l 24.9 25.2 24.7 25.0 24.7 7.45 7.27 7.32 7.28 7.42 6.85 7.28 7.20 7.39 7.8 7.7 7.8 7.4 7.8 6.3 7.6 7.7 7.9 300 321 321 309 314 64 64 64 87 95 89 24.9 2S.2 24.8 25.l 24.8 25.3 24.8 25.l 24.7 7.45 7.. 26 7.33 7.29 7.41 6.84 7.27 7.1.0 7.37 7.8 7.7 7.7 7.4 7.8 6.5 7.6 7.7 7.9 286 305 304 290 292 25.0 25.0 24.9 1.5.l 24,9 25.0 24.9 24.8 24.9 7.42 7.26 7.34 7.28 7.40 6.85 7.27 7.19 7.36 7.8 7.7 7.7 7.5 7.8 6.5 7.7 7.7 8.Q 263 282 283 271 279 25.0 25.0 2.5.0 24.9 24.9 25.3 24.9 24.8 24.9 7.41 7.25 7.33 7.28 7.39 6.89 7.24 7.19 7.34 7.9 7.7 7.8 7.6 7.8 65 7.8 7.7 8.0 230 242 242 234 239 25.0 253 25.0 25.2 25.0 25.0 2.5.0 25.0 24.9 7.41 7.27 7.33 7.22 7.39 6.92 7.23 7.19 7.33 7.9 7.7 7.8 7.6 7.9 6.4 7.9 7.7 8.0 212 222 221 217 217 25.0 25.0 25.0 24.8 25.0 25.0 25.0 25.0 24.9 7.42 7.28 7.34 7.27 7.38 6.89 7.23 7.20 7.33 8.0 7.7 7.9 7.5 8.0 6.3 7.9 7.7 8.0 194 202 198 196 198 78 74 74 83 83 83 <0.10 <0.10 <0.10 25.0 24.9 25.0 24.8 25.0 24.9 25.0 25.0 25.0 7.43 7.28 7.35 7.26 7.38 6.92 7.20 7.18 7.34 8.1 7.7 7.9 7.6 8.0 6.2 7.9 7.7 8.0 192 201 194 189 191 78 74 74 83 81 81 <0.10 <0.10 <0,10 25.0 24.9 25.0 24.9 25.0 24.9 25.0 25.0 25.0

• Note: Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.

19962 Project number: ________ _ Day S Day6 Final Initial Final Initial Final 7.11 7.61 7.SS 7.31 7.18 7.3 7.8 7.3 7.6 7.2 309 310 25.2 24.7 25.l 24.8 25,2 7.20 7.46 7.57 7.67 7,17 7.6 7.9 7.3 7.6 7.2 322 321 24.9 24.8 25.l 24.7 25.3 7..14 7.46 7.55 7.66 7.1.7 7.5 7.9 7.2 7.7 7.2 299 299 25.3 24.8 24.9 24.7 25.2 7.18 7.45 7.54 7.64 7.16 7.3 7.9 7.4 7.7 7.2 280 279 25.2 24.9 25.2 24.8 25.2 7.23 7.42 7.54 7.64 7.17 7.3 8.0 7.4 7.7 7.3 241 238 25.2 24.9 25.2 24.9 25.0 7.24 7.43 7.51 7.62 7.12 7.4 8.0 7.3 7.7 7.3 221 221 25.2 24.9 25.2 25.0 25.2 7.15 7.43 7.52 7.65 7.13 7.5 8.0 7.3 7.8 7.3 204 198 25.2 25.0 25.0 25.0 25.2 7.13 7.38 7.51 7.66 7.12 7.4 8.0 7.3 7.8 7.2 195 192 25.0 25.0 25.3 25.0 25.2 Entered bv: J. Sumner Page 39 of 93

Entered by: J. Perez Reviewed by: J. Sumner Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA, Sequoyah Nuclear Plant County: Hamilton NPDES #: TN 0026450 Outfall #: 101 Project #: \\ '\\'\\ {o "\\..- Treatment: None Dilution preparation: Page 1 of 7 Dilution prep (%) 17.25 34.5 69 84.S 100 Sample was not aerated or treated.unless otherwise noted Effluent volume (ml) 345 690 1380 1690 2000 on this form. Sample was warmed to 25.0 i 1.o*c in a Diluent volume (ml) 1655 1310 620 310 0 warm water bath and then diluted to the test Total volume (ml) 2000 2000 2000 2000 2000 concentrations with moderately hard synthetic water (MHSW). Test organism source: Test randomization and location: Organism age: < 24*hours old Randomizfng template color: &.L\\Je_ Date and times 011\\*ICI*'\\."' o~;o,() Incubator number organisms were born between: and shelf location: L. (., \\ Culture board: M* o**:r '--\\ A n'I-~*'-'\\ b Replicate number: l I 2 I 3 I 4 I s I 6 7 I a I 9 I 10 Culture board cup number: 1.11 "le l"l\\ I~"\\. I~ I '10,, \\ I "l-1 <; I 10 Transfer vessel information: pH (S.U.): "\\. ""'L Temperature (°C): "\\$. 0 ' ( Average transfer volume {ml): <0.25 ml Daily renewal: Day Date Test initiation and feeding,

• Feeding Batches MHSW Sample numbers used Analyst renewal and feeding, Or Selenastrum YWT batch used Outfall 101 Intake termination time 0

09-10.24 l)&~R' 0"\\-()1,*'\\.'\\ (A-olo* \\.~ O\\*o\\\\* '\\.'\\ A. "'\\.t.\\o<\\OC\\.o~ ~ o'\\o'i. t7'-\\ \\,t 1 09-11-24 OJ('t L

l.

1 i 2 ~ 09-12-24 0&10 o'\\-~*'-'-']:) 1.-1 O'i i\\. !,b 1.-\\ot,\\\\~1 IA 3 ~ \\.. l 09-13-24 Or 10 4 09-14-24 d 0. C\\,._ 04-*o\\*1.'-\\ ~ l l.l Nl,._. 1.J.. 'l.-\\0'\\ I'\\. 1.i k( s 09-15-24 o°'~ \\ \\ \\ 4t 6 09-16-24 0104

l.

l j, .J,{ 'u ~ 7 09-17-24 61.'-li Ji L 1, ---, l

• Organisms fed dally 100 µLSe/enastrum and 100 µL YWT per replicate using HandyStep repeat pipettor SN 17ES93S4 Chemical analyses:

Parameter Reporting limit Me.thod number Meter Serial number pH 0.15.U. SM 4500-H+ B-2021 Accumet AR20 93312452 Dissolved Oxygen {D.O.) 1.0 mg/L SM 4500-0 G-2021 YSI Model 52CE 08A100271 Conductivity 14.9 µmhos/cm SM 2510 8-2021 Accumet AR20 93312452 Alkalinity 5.0 mg CaCO,/l SM 2320 B-2021 Accumet AR20 93312452 Hardness 5.0 mg CaCO,/l SM 2340 C-2021 Not applicable Not applicable Chlorine, Total Residual 0.1 mg/l ORION 97-70.1977 Accumet AB250 92349123 Temperature 0.1 *c SM 25508-2010 Digital Thermometer '~O'-i~l..f.!; Control information: Control-1 Control-2 Acceptance criteria Summary of test endpoints: % of Male Adults:

01.

01.

S20% 7-day LCso (%) '>,er/I. % Adults having 3rd Broods: /007, ,oo 7. ~ 60'1/4 surviving adults NOEC (%) 1001. % Mortality:

01.

01.

~20% LOEC (%) ') l{)C) 1. Mean Offspring/Female: ~ . 1. ~o.1o

?c 15.0 offspring/female ChV(o/o)

) IM f. IC.25 {%) '> 1001. SOP ATll-Revision 6-Exhibit AT11.2 Page 40 of 93

Entered by: J. Perez Reviewed by: J. Sumner _\\ _ _ _ "'"""~t.J lfl'Uflt SaM_,r. lM-Page 2 of7 Species: Ceriodaphnia dubia Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 09-10-24 CONTROL-1 Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 l Young produced 0 0 0 0 0 0 D 0 () 0 Adult mortality L- \\._ L \\..... L L L L 2 Young produced 0 C ("--., 0 0 0 D 6 0 () Adult mortality L \\._ '-- 3 Young produced 0 () 0 C C> C> (') D 0 D Adult mortality L \\..... \\....... \\..... \\._ L. L 4 Young produced '-\\ ~ ~ (o -=-:, '4 ~ ~ '-{ '--l Adult mortality L \\...... L.. \\....... L L \\.._ 5 Young produced JO \\L,. 11.. ~ lD ID '\\ \\ "\\... IC) r D Adult mortality L L L \\..._ 6 Young produced 0 0 a 0 0 0 0 0 0 D Adult mortality \\..... '-- \\........ '-- \\.._ 7 Young produced \\S. 1 ':) I:> l\\o \\1,s I'-\\ l &, ' '--\\ \\ \\o Total young produced 1.c; ~, ~b ~\\ '?),_,_~ '1..1\\ ~~,_t ~t:) Final Adult Mortality )( for 3"' Broods 't<- "')(:'._ "'),(__ )C'.:.. ~ ~ ~ ~ ;;><- No<<: Adult mortality (L; IIY*, 0 = dHdl, SB= 1pllt brood l*lnsl* brood split between two d1y,), CO* corry over loffspnn1 a,rrled ovu with 1dult durln1 tr.insfor), Concentration: % Mortality: I

07.

Mean Offspring/Female: I ~-"'L CONC: 17.25% Survival and Reproduction Data Replicate number Day l 2 3 4 5 6 7 8 9 10 1 Young produced 0 0 t) (\\ n 0 0 0 n 0 Adult mortality L. '-- \\..- \\_ L L L L \\...__ 2 Young produced {'I (} {) () l"i 0 0 b 0 C) Adult mortality \\,_ 3 Young produced (\\ {'j 0 C> (', 0 0 0 C> 0 Adult mortality L.. L L L L L 4 Young produced ~ ~ '4 s

u.

y '-\\ y 4 Adult mortality L-L L L L L L s Young produced ,o \\~ I C\\ I\\ \\:) 10 I ~ I \\ l \\ \\\\ Adult mortality L \\_... '-- L \\..._ 6 Young produced 0 0

r.

('I 0 n 0 0 0 0 Adult mortality L \\_ '- '- L L \\..._ L 7 Young produced IS,, \\&, t 4 \\ °' \\\\ 11 1 '-l I I,. Total young produced ~'t) ~, ~'"L ~\\ ~., ~, ~'\\ -i_, ~, YL. Final Adult Mortality l \\ l L \\,_ Note: Adultmort1ifity (l; live, 0.::1 dead), SB :..spHt brood (s1n1le brood ~pUt between two diitVS), CO i:: c*rrv over (offspnnc ntried ovuwfth ~dult durinc tri:ruJer} Concentration: % Mortality: 07, Mean Offspring/Female: ,'2,.1.. '-I % Reduction from Control-!: 311. SOP ATll-Revision 6-Exhibit ATll.2 Page 41 of 93

Entered by: J. Perez Reviewed by: J. Sumner [Mlrllltfflllll'bl 1Htll'lf Sahna.11111, lfll'. Page 3 of 7 Species: Ceriodaphnia dubia Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 09-10-24 coNc: 34.5% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 C f) 0 D () () a 0 0 Adult mortality ~ \\_ \\_ \\....- '- L. L. '- L L 2 Young produced n D 0 C) 0 6 D t'i (:\\ C) Adult mortality \\.._ '- \\......- \\....- L 3 Young produced 0 0 0 n 0 0 0 0 0 0 Adult mortality L- \\.._ L.. \\_ L. L \\._ L. '- 4 Young produced '--\\ '-\\ ~ "° ~ ~ ~ s ~ ~ Adult mortality \\_. \\...... \\..... \\..... s Young produced 1\\ \\.,_ I"\\. I'-\\ IQ I t-i \\\\ \\~ I~ \\"l-Adult mortality ~ \\_ '- \\..__ 6 Young produced CJ () r-i 0 0 L) a 0 0 0 Adult mortality L '- L. \\.._ L- '- \\.. 7 Young produced \\ <::, l ':, I°\\ l <\\ I f-1-0 'k,s IC\\ 1...\\ Total young produced ~t) ~\\ .}S ~Ii. ~, ~s b~ ~~ ?)"'\\ ~~ Final Adult Mortality \\. \\_ Not~: Adult mort,11/tv IL= l1ve1 0 ~ dead), SB:;. spilt brood I.Sin&!* brood ss;iUt between two days I, CO.:. uny over (offspnnc carried over with 1dult durln1 tr.antfer) Concentration: % Mortality:

01.

Mean Offspring/Female: ~4."1-o/o Reduction from Control-1: -,~. i.7. CONC: 69% Survival and Reproduction Data Replicate number Day 1 2 3 4 s 6 7 8 9 10 1 Young produced () (1 () rt-0 6 0 0 0 0 Adult mortality l-L. L L L.. L L 2 Young produced 0 0 0 C f) f) Q 0 ("\\ 0 Adult mortality \\ \\,_ \\..- \\.._ L- \\..... 3 Young produced () 0 () () () 0 /) (j 0 0 Adult mortality \\._ L L L L L L.. L 4 Young produced I.:, ':) I.:, '-\\ ~ '-\\ '-\\ ~ s Adult mortality \\.._ '- 5 Young produced 11.. '~ \\\\ ( '-I \\~ *~ I \\ It) I-,_,, Adult mortality L L.. L \\. L L L L \\_ 6 Young produced a 0 0 0 0 0 u {) 0 0 Adult mortality L \\.._ '- '- \\._ L. ~ '- L \\,._ 7 Young produced 1..C) ,a ,~ \\ '\\ -Z.\\ l 't,,,., 1.o Total young produced ~s ~4 ~s 31 ~& 3~ ~~ 31 ~~ ~8 Final Adult Mortality \\_ '-- \\. \\._ \\.._ L I....._ Not@: Adult mortalltv IL: live, D :::: dead)i 58: spllt brood h*lncle brood spht be.tween rwo diys:)1 CO = c~rry o<,oer (ofb prin1 cu ri,d over with ~dult dur1n1 tn rufer) Concentration: % Mortality: Ol. Mean Offspring/Female: ~-1 % Reduction from Control-1: -,&. 1. 7. SOP ATll-Revision 6-Exhibit AT11.2 Page 42 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 4 of7 Species: Ceriodaphnia dubia Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 09-10-24 coNc: 84.5% Survival and Reproduction Data Replicate number Day 1 2 3 4 s 6 7 8 9 10 1 Young produced () a () 0 0 0 C) n 0 0 Adult mortality L.,.. L-L. L..- L.- \\_.. L. L L L 2 Young produced (',.. (') D C) C) (\\ a f\\ {) t\\ Adult mortality L L- \\,_ L L 3 Young produced r, () 0 0 r-i C) 0 0 0 0 Adult mortality L L-L. L \\... L L L L.. 4 Young produced ~ b ~ ~ ~ ~

u.

'-\\ '-\\ ~ Adult mortality \\_ \\.-..:. L s Young produced \\~ \\ \\ -\\ '1.. l' I\\ 11.... '1. I\\ Adult mortality L- '- L-6 Young produced ("\\ () (\\, () (') 0 0 a C) C Adult mortality \\_. L \\_ L \\,_ 7 Young produced \\D\\ I 4\\ -L\\ \\ '\\ l '° I '- \\ '\\ ""Lb \\ fr Total-young produced ~, "!,~ '4~

,1,.

~'\\ ~,. ~'S ~ \\., b'\\ ~~ Final Adult Mortality L. l.._ Note: Adult mortal*tv (L = live, D :E cfud)1 SS.= spJlt brood (smile brood spllt between two d\\lys), CO ~ arry over (offsprin1 a rried over with *dult durlnc t~nsfer} CQncentration: % Mortality: ()1. Mean Offspring/Female: "!5.11, % Reduction from Control-1: - l lo.°I 1. CONC: 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 s 6 7 8 9 10 1 Young produced n () a '() {) 0 /) 0 0 0 Adult mortality L. L... L- \\_ L.. \\.._ 2 Young produced 0 0 C) 0 D a C 0 C 0 Adult mortality L- '- \\._ L 3 Young produced 0 0 0 0 0 () 0 0 0 0 Adult mortality \\.... '- L. '- L L \\_ L L 4 Young produced

s.

~ ~ '-\\ \\, ~ ~ ~ ~ <. Adult mortality L L 5 Young produced 10

• '-l 1.'.'\\

11.

I:!) '~ 1'-t \\\\ '"' Adult mortality \\. \\_. \\.... L L \\.... 6 Young produced f) 0 ('"') 0 0 0 n 0 n 0 Adult mortality L. \\.._ \\_ '- L 7 Young produced '--l *~ ' tr 'lb '°' lS \\ '\\ \\1 IC\\ l..O Total young produced ~lo

!,1

~lo ~\\o 3,~ ~'\\.. ~~ 3~ ~& ~1 Final Adult Mortality \\....... \\.. Nor~; Adull mort11itv (le l1ve1 O =-du d), SB =.split brood (r1n1l1t brood split be-twu n two d1y1)1 CO= carry ovu toff1prln1 urned over Wlth ~dull dunn1 tRn.der) Concentration: % Mortality:

01.

Mean Offspring/Female: .Jlo, 2 % Reduction from Control-1: - I'\\., 7. SOP ATH -Revision 6-Exhibit AT11.2 Page 43 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 5 of 7 Species: Ceriodaphnia dubia Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date:,09-10-24 CONTROL-2 Survival and Reproduction Data Replicate number Day 1 2 3 4 s 6 7 8 9 10 1 Young produced 0 0 (') D 0 0 D Q 0 () Adult mortality L \\..- L. L. l-L... 2 Young produced r> 0 D a 0 (') b 0 0 0 Adult mortality ~ \\..... '- \\_ \\......._ 3 Young produced 0 {) {) 0 (') 0 0 0 0 /) Adult mortality \\_. \\..- L L L 4 Young produced c; "-'\\ ~ 4 '--\\ '--\\ \\.,

u.

~ '-t Adult mortality L \\ 5 Young produced /0 \\ 1- \\ \\ \\1:) I-,_

• o t 0 I l I()

lO Adult mortality L \\..... \\..... '- 6 Young produced 0 n r--. 0 0 0 0 () C 0 Adult mortality \\. \\. L. '- L I.._ 7 Young produced 11\\. I '-l -\\ l l, IL, \\ ~ 1l.,, I(., Total young produced "b'-\\ !I,,..;/',.: t L.f ~\\. ~'l..

!)b l.C, ~,

~\\ !:, D '.7 _;;- Final Adult Mortality L ~ '- X for 3"' Broods '>(_ ""I'- 7'- -r-- ')C-- )<.. ')C-Y-- )C._ Nott: Adult mort1Uty IL a: live, 0 = de,d), SB x split brood (sfngle brood split btitween two di vs). CO= cury over (off1prin1 c1rri1d o-.er with 1dult durfnc t~n.sf*r). Concentration: % Mortality: I

01.

Mean Offspring/Female: I '30,\\o coNc: 100% Intake Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 a 0 A 0 c) 0 0 0 0 Adult mortality L L L L. L L L 2 Young produced ('\\ 0 0 (""\\ (\\ ('"\\ ('\\ {"\\ 0 0 Adult mortality I L \\_ \\_ '- '- 3 Young produced () C) 0 0 0 0 0 0 0 0 Adult mortality \\_ \\..... '-- L L. '--- 4 Young produced '° s I... \\., \\., s ~, 5 Adult mortality \\._ \\_, '- L L s Young produced l'-{ L\\ \\.,_ I~ It) \\ "'- I l q

14.

10 Adult mortality L \\..... \\..... \\..... L l__ 6 Young produced C) 0 0 0 0 0 0 0 0 0 Adult mortality \\._ \\,_ '\\_ '- \\.._ L 7 Young produced I':) ,s ' '\\ I~ "'Lb \\ t ' ""\\ 11 '1 17 Total young produced 1>':. ~, ~"\\ ~& ~~ ~ \\. ~~ ~s ~\\., ~"'l..... Final Adult Mortality \\_. L \\..... L \\..... \\____ Nr,te: Adult mortality (l ~ live, 0 :-dud), SB =-.1pUt brood (slnale brood split bt twrrn two days), CO: ~rry over (offsprinE Cirrted ovtrwith 1.dult durlna l~nsfer). Concentration: % Mortality:

01.

Mean Offspring/Female: 3"/, (, % Reduction from Control-2: -1!1,11. SOP ATll-Revision 6-Exh ibit: A Tl 1'. 2 Page 44 of 93

Entered by: J. Perez Reviewed by: J. Sumner l E.nllhonm;ml,11 T*ltfl\\9 SoklOoh,, ~C. Control-1 ~ Day Replicate number 1 2 3 4 5 6 7 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 4 4 5 6 5 4 4 5 10 12 12 9 10 10 11 6 0 0 0 0 0 0 0 7 15 15 13 16 17 15 14 Total 29 31 30 31 32 29 29 17.25% Day Replicate number 1 2 3 4 5 6 7 l 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 s 5 4 6 5 4 4 5 10 13 10 11 -13 10 13 6 0 0 0 0 0 0 0 7 15 19 18 14 19 17 17 Total 30 37 32 31 37 31 34 34.5% Oay Repllcate number l 2 3 4 5 6 7 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 4 4 4 6 3 5 4 5 11 12 1Z 14 10 10 u 6 0 0 0 0 0 0 0 7 15 15 19 19 18 20 18 Total 30 31 35 39 31 35 33 69% r* oav ReollCiltC. number 1 2 3 4 5 6 7 l 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 6 s 6 4 6 4 4 s 12 13 11 14 1.3 13 11 6 0 0 0 0 0 0 0 7 17 20 18 19 19 2l 19 Total 35 38 35 37 38 38 34 TVA/ Sequoyah Nuclear Plant, Outfall 101 September 10-17, 2024 Verification of Ceriodaphnia Reproduction Totals 84.5% 8 9 10 Total Day l 2 3 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 3 4 4 43 4 5 3 5 12 10 10 106 s 13 11 14 0 0 0 0 6 0 0 0 18 14 16 153 7 19 19

21.

33 28 30 302 Total 37 33 40 100% 8 9 10 Total Oay

l.

2 3 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 4 4 4 45 4 5 5 5 11 11 11 113 s 10 l4 13, 0 0 0 0 6 0 0 0 14 16 17 166 7 2l 18 18 29 31 32 324 Total 36 37 36 Control-2 ~ ~ 8 9 10 Total Oay 1 2 3 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 5 5 5 45 4 5 4 4 13 13 12 118 s 10 12 11 0 0 0 0 6 0 0 0 15 19 21 179 7 19 14 13 33 37 38 342 Total 34 30 28 100% Intake ---~- *--~---- 8 9 10 Total Day l 2 3 0 0 0 0 l 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 4 4 5 48 4 6 5 6 10 12 13 122 s 14 11 12 0 0 0 0 6 0 0 0 17 17 20 187 7 15 15 19 31 33 38 357 Total 35 31 37 Replicate number 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 6 5 4 12 13 u 12 0 0 0 0 19 18 16 19 36 37 32 35 Repllcate number 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 4 6 4 6 12 13 l3 14 0 0 0 0 20 19 15 19 36 38 32 39 Reollcate number 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 4 4 4 6 13 -12 10 10 0 0 0 0 14 16 16 13 31 32 30 29 Replicate number 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 6 3 6 s 13 10 12 11 0 0 0 0 19 20 18 17 38 33 36 33 8 9 0 0 0 0 0 0 4 4 12 12 0 0 20 18 36 34 8 9 0 0 0 0 0 0 5 5 11 i4 0 0 17 19 33 38 8 9 0 o, 0 0 0 0 4 4 11 10 0 0 16 17 31 31 8 9 0 0 0 0 0 0 5 7 11 12 0 0 19 17 35 36 10 0 0 0 4 11 0 18 33 10 0 0 0 5 12 0 20 37 10 0 0 0 4 10 0 16 30 10 0 0 0 5 10 0 17 32 Tota) 0 0 0 45 121 0 187 353 Total 0 0 0 50 126 0 186 362 Total 0 0 0 43 109 0 154 306 Total 0 0 0 54 116 0 176 346 ,(=.-= ~T Page 45 of 93

Entered by: J. Perez Reviewed by: J. Sumner Environmental Testing Solutio ns, Inc. Concentration (%) 1 2 3 Control - 1 29 31 30 17.25% 30 37 32 34.5% 30 31 35 69% 35 38 35 84.5% 37 33 40 100% 36 37 36 Control* 2 34 30 28 100% Intake 35 31 37 Outfall 101: Ounnett's MSD value: 2.526 PMSO:

8.4 Intake

Ounnett's MSO value: 1.538 PMSO: 5.0 TVA/ Sequoyah Nuclear Plant, Outfall 101 September 10-17, 2024 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 4 31 31 39 37 36 36 31 38 MSD = PMSD= Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 19962 Replicate number Survival Average reproduction Coefficient of Percent reduction from s 6 7 8 9 10 (%) (offspring/female) varlatlon (1'1 pooled controls {%1 32 29 29 33 28 30 100 30.2 5.1 Not applicable 37 31 34 29 31 32 100 32.4 8.S -7.3 31 35 33 33 37 38 100 34.2 9.1

• 13.2 38 38 34 31 33 38 100 35.7 7.0

-18.2 37 32 35 36 34 33 100 35.3 6.8 -16.9 38 32 39 33 38 37 100 36.2 6.1 -19.9 32 30 29 31 31 30 100 30.6 5.4 Not applicable 33 36 33 35 36 32 100 34.6 6.6 -13.1 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant In a whole effluent to)(icity test. Lower PMSD bound determined by USEPA (lO'h percentile) = 13%. Upper PMSD bound determined by USEPA (901h percentlle) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WfT lnterlaboratory Variability Study (USEPA, 2001a.; USEPA, 2001b). US EPA. 2001a, 2001b. Final Report: lnterlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxiclty Test Methods, Volumes 1 and 2-Appendix. EPA-821-8-01-004 and EPA-821-B-01-005. lJS Environmental Protection Agency, Cfncihnati, OH. ~*-... .,,~.. -r Page 46 of 93

.) Environ mental Testing Solu:lo~~, Inc. Start Date: 9/1012024 TVA/ Sequoyah Nuclear Plant, Outfall 101 September 10-17, 2024 Statistical Analyses Cerlodaphnla Survival and Reproduction Test-Reproduction Test ID: CdFRCR Sample ID: TVA/SQN Outfall 101 End Date: 9/1712024 Lab ID: ETS-Envir. Testing Sol. Sample T~e: ~- Discharge Monitoring Report Sample Date: September 2024 Protocol: FWCHR-EPA-821-R-02-013 Test Species : CD-Ceriodaphnia dubia Comments: Cone-% 1 2 3 4 6 6 7 8 9 10 Control-1 29.000 31.000 30.000 31.000 32.000 29.000 29.000 33.000 28.000 30.000 Control-2 34.000 30.000 28.000 31.000 32.000 30.000 29.000 31.000 31.000 30.000 17.25 30.000 37.000 32.000 31.000 37.000 31.000 34.000 29.000 31.000 32.000 34.5 30.000 31.000 35.000 39.000 31.000 35.000 33.000 33.000 37.000 38.000 69 35.000 38.000 35.000 37.000 38.000 38.000 34.000 31.000 33.000 38.000 84.5 37.000 33.000 40.000 36.000 37.000 32.000 35.000 36.000 34.000 33.000 100 36.000 37.000 36.000 36.000 38.000 32.000 39.000 33.000 38.000 37.000 Intake 35.000 31.000 37.000 38.000 33.000 36.000 33.000 35.000 36.000 32.000 Transform: Untransformed 1-Talled Isotonic Cone-% Mean N-Mean Mean Min Malt CV% N t-Stat Crttlcal MSD Mean ~*an Control-1 30.200 0.9869 30.200 28.000 33.000 5.130 10 34.000 1.0000 Control-2 30.600 1.0000 30.600 28.000 34.000 5.381 10 17.25 32.400 1.0588 32.400 29.000 37.000 8.509 10 -1.992 2.287 2.526 34.000 1.0000 34.5 34.200 1.1176 34.200 30.000 39.000 9.122 10 -3.621 2.287 2.526 34.000 1.0000 69 35.700 1.1667 35.700 31.000 38.000 6.993 10 -4.979 2.287 2.526 34.000 1.0000 84.5 35.300 1.1536 35.300 32.000 40.000 6.816 10 -4.617 2.287 2.526 34.000 1.0000 100 36.200 1.1830 36.200 32.000 39.000 6.080 10 -5.432 2.287 2.526 34.000 1.0000 Intake 34.600 1.1307 34.600 31.000 38.000 6.562 10 Awdllary Testa Statistic Critical Skew Kurt KolmogorovD Testindicates normal distribution (p > 0.01) 0.6569 1.035 0.12887 -0.5365 Bartlett's Test indicates equal variances (p = 0.50) 4.37292 15.0863 The control means are not significantly different (p = 0.58) 0.5595 2.10092 Hypothesis Test(1 -tall, 0,06) NOec LOEC ChV l\\J MSDu MSDp MSB MSE F-Prob df Ounnett's Test 100 >100 1 2.52571 0.08363 52.92 6.1 4.4E-06 5, 54 Treatments vs Control-1 Linear lnterpolatlon (200 Resamplea) Potnt SD 96%CL Skew IC0S >100 IC10 >100 IC15 >100 1.0 IC20 >100 0.9 IC25 >100 0.8 IC40 >100 0.7 IC50 >100 0.6 0.5 .. 0.4 §_.. 0.3 0.2 0.1 0.0 -0.1 -0.2 -0.3 0 so 100 150 Dose% Dose-Response Plot 45 40 I I I 35 T l I l -- 30 _______ j _____________________________________________________ 1-tail, a.as level !i ~ 25 of significance {20 &! 15 10 5 0 ~ N m § I ] N ~ <D

t s

s ~ 0 0 Page 47 of 93

Entered by: J. Perez Reviewed by: J. Sumner Ill Envrronm4!nt31 Te-sting Solution§, Inc. Start Date: 9/10/2024 TVA/ Sequoyah Nuclear Plant, Outfall 101-Intake September 10-17, 2024 Statistical Analyses Ceriodaphnla Survival and Reproduction Test-Reproduction Test ID: CdFRCR Sample ID: TVA I SQN Intake End Date: 9/17/2024 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: September 2024 Protocol: FWCHR-EPA,821 -R-02-013 Test Species: CD-Cerlodaphnia dubia Comments: Col'IC'-% 1 2 3 4 5 6 7 8 9 10 Control-1 29.000 31.000 30.000 31.000 32.000 29.000 29.000 33.000 28.000 30.000 Control-2 34.000 30.000 28.000 31.000 32.000 30,000 29.000 31.000 31.000 30.000 17.25 30.000 37.000 32.000 31.000 37.000 31.000 34.000 29.000 31.000 32.000 34.5 30.000 31.000 35.000 39,000 31.000 35.000 33.000 33.000 37.000 38,000 69 35000 38.000 35.000 37.000 38.000 38.000 34.000 31.000 33.000 38.000 84.5 37.000 33,000 40,000 36.000 37.000 32.000 35.000 36,000 34.000 33,000 100 36.000 37.000 36.000 36.000 38,000 32,000 39,000 33.000 38.000 37.000 Intake 35.000 31.000 37.000 38.000 33.000 36.000 33.000 35.000 36.000 32.000 Transform: Untransformed 1-Talled Cone-% Mean N-Mean Mean Min Max CV% N t--Stat Crltlcal MSD Control-1 30,200 0.9869 30.200 28.000 33.000 5.130 10 Control-2 30.600 1.0000 30.600 28.000 34.000 5.381 10 17,25 32.400 1.0588 32.400 29.000 37.000 8.509 10 34.5 34.200 1.1176 34.200 30.000 39.000 9.122 10 69 35.700 1.1667 35.700 31,000 38.000 6.993 10 84.5 35.300 1-1536 35.300 32.000 40.000 6.816 10 100 36.200 1.1830 36.200 32.000 39.000 6.080 10 Intake 34.600 1.1307 34.600 31.000 38.000 6.562 10 -4.510 1.734 1.538 Auxiliary Tests Statistic Crltlcal Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96577 0.868 0.05073 -0.4619 F-Test indicates equal variances (p = 0.35) 1.90164 6.54109 The control means are not significanUy different (p"' 0.58) 0.5595 2.10092 Hypothesis Teat (1-tall, 0.05) MSOu MSDp MSB MSE ~Prob df Homoscedastic t Test Indicates no significant differences 1.53801 0.05026 80 3.93333 2-7E-04 1, 18 Treatments vs Control-2 Dose-Response Plot 45 40 I T I 35 rd===-- l I l---. 30 ***-*-1 ---- -------------------------------------- 1-tail, 0.05 level C: of significance .2 g 25 I 20 ~ 15 10 s 0 ~ ~ 0, ~ j J. N ~ tO _g g 8 8 ~ ..5. Page 48 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 6 of7 Species: Ceriodaphnia dubia Date: 09*10-24 Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Daily Chemistry: Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information t able located on Page 1. Alkalinity, hardness and chlorine (total residual) performed by the analyst Identified on the bench sheet specific for each analysis and transcribed to this bench sheet. Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 0 1 2 Analyst I/\\- J%_,.,,,,J\\., {fo<-1 /lJ G/J Vv A-Y'-- ii--' Concentration Parameter pH(S.U.) ,~_':)-, 7, 2-"$ 7,LI-9 ?, i. 'b ?,?'2.. /l..21 Dissolved o,cygen (mg.IL) ,:: :i

• u,

~~ /}8 '+-6 f.},u,

CONTROL, Conductivity (µmhos/cm)

Z'tt ~~o <I~ MHSW Alkalinity (mg CaCOJL) lo'

• .. ;.i~;....

\\,'-\\ Hardness (mg CaCOJL) ~ al\\ Temperature (*C) '"\\.~'\\ "'\\.C.* L '"\\.'\\." "\\.'i.1., '"\\,.-\\, i "\\.S. \\ pH(S.U.) /'.l-. '50 7 -z...3

7. 7,.7

?,2g> ?,$C) 't,2.l 17.25% Dissolved o,cygen (mg.IL)

• -t"\\--

'~.,(, ~~ 'l-01 ~+.1 -, }.(I Conductivity (µmhos/cm) 26\\- so<=1 3o7-Temperature (°C) ""\\S. t) ~-'L.. "\\.C':., t) "'\\S,.O 1A.. 'i '\\.<,. \\ pH(S.U.) ..J, "1 (,, 7, 2-1,

7. 2.S?

7, fl "7.~-Z.... ".)..20 Dissolved oxygen (mg/L) ~-"'

• ~M "lA

'+ 'i --t) 1\\..11\\ 34.5% .,.,c '29D Z<;JLI-Conductivity (µmhos/cm) Temperature (*C) ""\\S. '{) ~ - \\.. "\\S.

• Tl

"\\S. 0 "'\\.S. t) '\\.C,. ~ pH(S.U.) 'l'-lu 7.ztP

• 7 70 7, ?'2...

7,?2- '4-11'.\\ 69% Dlssolved oxygen (mg.IL) ~8 /lR '+~ ...... -1/4".:l ~ /11 Conductivity (µmhos/cm) 21'l 2#9< 'VI-Z.. Temperature (*C) ~ . t) "U-"1- ,<.. 0 i..~-~ '1..S,0

• v *. o pH (S.U.)

'.4~ 7, 2-~ ?. 2 9 7.~'2 7.Jz 4.A 84.5% Dissolved o,cygen (mg.IL) '1.t:\\

• 4,1, l'}_d 1\\-.lt

--t-8 ~(p Conductivity (µmhos/cm) '2 If\\ -Z..7-CJ 2-v-f Temperature ('C) "\\..',.'() "\\.~- ~ "\\.':>>

• t>

"'\\.~- ~ "\\5., D ""'. a. pH(S.U.) Y-l,\\.f C.., 7.2. 9 ? 733

7. 12..

I+. A Dissolved o,cygen (mg.IL) 8/J /l,,ts ,..,,,__., ~,o q.c.; -:\\,,')- Conductivity (µmhos/cm) IQ:.B L..L () 1-f:JO 100% Al~aflnlty (mg CaC01/L) -"\\t ,c ,'-\\ Hardness (mg CaCO,/L) 81 f l -:.~ i, Chlorine (mg.IL) <: C)-1'0 = ~ -4 0.10 Temperature ('C) ~ -0 .., !.. \\ u.o i.{, "L. "l.<.-0 "\\.S-!, pH(S.U.) ~.4} 7, 2-fd

7. so 7 '3 'f

7. 5'3

~ I\\ Dissolwed oxygen (mg/L) Pu fl..~ /l.1 e, 0 ~o "+,".} 100% Conductivity (µmhos/cm) IAO. 2.&7 I')$=! Intake Alkalinity (mg CaCO,/L) ii ,-t Hardness (mg CaCO,/L) I~ 11 i\\ Chlorine (mg.IL) <. 0.,o <.. 0. l() Temperature ('C) "\\.S,O "\\.<.. 0 lS,0 "\\_~. '\\. ,.~.o "\\ <:..,.,.. Initial Final Initial Final Initial Final SOP ATll-Revision 6-Exhibit ATll.2 Page 49 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 7 of 7 Species: Ceriodaphnia dubia Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 09-10-24 Day (Analyst identified for each day, performed pH, 0.0. and conductivity measurements only.) 3 4 5 6 Analyst I.A-O.c.1..., P.st..- e,~c, &:,~ ~,,,/4--? g,,,.,,,, l'/f.-1 Concentration Parameter pH (S.U.) ~YZ. 1,ig J..43 ~-; J,ul 7,67 7.1/ 7,'f-o Oluolvtd OIIV,tn {mr/1.1 1-,°I -:i. 'l ,.9 l~

7. l\\

1-b

CONTROL, Conductivity (µmhos/cm) 3011 3lv 3,,,

3'/D a I* MHSW Allullnlty {me C.CO,/l) r---..... 1: .*k ' ~" 1, ~ HO!dntss (mc C.CO,/l) ~ ...---i 81 ........... - --~ T t mptt"ltUft 1°C) "--~. °' "\\.',- ~ '\\.--\\.t "-C-. - ~ i.'\\. C, "\\S. 'L '\\.'-t-cf

• v.. \\

pH{S.U,1 r.J.1+ 1-.~~ 1-,BIP ~-43

J."'-t 7.59

'7 J., ~ 7,39 Dluol**d oxv,tn lmc/ll '+1 1-,C\\ '1*1 1,9 t,1' ,.'\\ ,.7 ""\\. ~ 17.25% ~~ -<. "I :I, .. H1-300 Conduclivity {µmhos/cm) Ttmptratur* re) '\\."I,\\ '\\.S,.o '\\.."\\,'\\ ~-'\\... "'\\."\\. '\\ \\.S.. 0 "\\).l. ~ "'\\.'>. ~ pH (S.U,) '1.~ 1-,;i 'l-, 38

J.~o 1.4'6

?. S'J 7, 6'2. 7 -19 Ol1solved O"YCtn {me/LI

• ii

~~ct -:i '& +,'\\ +t B, C) 7.7. -~ 34.5% ii<1o ~14 ~':?1

i. 9 t.f Conductivity lµmhos/cml Te..mper*nue (°C)

~ -0 "--'-. '\\.. "\\.C.. 0 -u.. "\\., "\\.'i.. 0 \\S."!, 1.'-(.'\\ "\\.S.~ pH(S.U.) '\\,t.\\&> l-,'3i "l,3 lo 1-~

}.~

7,6o 7,6-Z... ?-38 DlssolYtd oxv,*n (mc/l)

• ~.e

-:}A t,i ""* "I -i,q "1.°\\ ,.7 ,.i 69% ~13 ~3~ .:23'1 1....39 Conductivity (µmhos/cml Tomp,ratutt l"CI '"\\.S. 0 'U*"'- 1.S-O \\.~. '-\\ "\\.~-o '\\S* '\\.. ,s, 0 ~-~ pHIS,U.) '-}.2.5 °l,'3"l- -:J, 35 , 1, 5 1.'+:5 7.6o 7,6/ 7.3':J Dhsolved O"YJ*n (me/LI '-\\-9 ':l-,°1

i,9 1.<i,

'l.'l 1.', i,7 ~-, 84.5% 2,Pi .;I 15

• -z, 7.. I Conduclivlty (µmho1/cm)

~' Ttmptraturo rq '\\.S-0 '\\.!..0 "\\.S-O "'\\.S,\\ 1.~-0 "\\S* 'l.. "\\S.o \\.~.~ pHIS.U.) 4,22 l-,-a1- ~-a~ -:}-A5' '.l.45 7,62. 7,b/ 7,58 Dlssol**d oxvs*n lmc/l) '1-.9 l."l

J.1 1~

~-q 7.4\\ "l. '1 ,.'\\ ConductMty (µmho,/cm) l':11"" .;}ob ~~~ ... ~-- 20~ ,'-\\

-_::. -* -~, F

~ -~.. 100% Alkalinity ( mg c,CO,/l) Hudntss lm1 c.co,/1.) 'L - - --

. I "'

<- o, *O Chlor!nt (mc/l)

• -1' T*mptnturo ("C)

"\\.S. 0 ~~-0 "\\.<.. D \\.SA "\\~. 0 "'\\!;.. "L "\\.S, 0 "\\.t;. 0 pH (S.U.) 1.2v/ 1-1-.34

J.,5

+A"1 ?.ti '7, t 1._ 7.t.&o Oluolv*d o,yg,n (me/LI 'i9 +>1

i.q

':#,'i 1'"-'l ,.~ ,. g-- .r. D Conductivity [µmhos/cm) \\(.\\1 I 'i l4> cl~ ") rlJ. 100% ,'-\\ Intake Alkallnlty (mg CaCO,/ll .II Hudnts> (m1 CaCO,/L) '°' Chlorlnt (mc/l) ,(, o.,o T*mp,roturt {"C) "\\4.b "\\.<.. t) \\.S.o "\\.':.. 'L "l.S. O "-f-- 0 \\S. 0 "\\.<,,."L Initial Final Initial Final Initial Final Initial Final SOP ATll-Revision 6-Exhibit ATll.2 Page 50 of 93

Entered by: J. Perez Reviewed by: J. Sumner Environmental Testing Solutions, Inc. Concentration Parameter DH (SUI DO (me/L) Control Conductivitv (umhos/cm) Alkalinity (mg/L caco,) Hardness (mg/L caco,) Tem0erature (°C) pH(SU) 17.25'1/4 DO (ml!.fLI Conductivitv fumhos/cm) Temperature (°C) DH (SU) 34.5% DO (mg/LI Conductivity h1mhos/cm) Temperature (°C) pH(SU) 00 (me/LI 69% Conductivitv lumhos/cm) Temperature (°C) IPH(SU) 00 (ml!/Ll 84.5% Conductivity lumhos/cml Temperature (°C) pH(SUI DO(ml!.fL) Conductivity (11mhos/cml 100"/4 Alkalinity (mg/L CaCO,) Hardness (mg/L CaC0,1 Total Residual Chlorine (m2/Ll Temperature (°C) oH(SU) DO (m2/Ll Conductivitv (µmhos/cm) 100% Intake Alkalinity (mg/L CaCO,) Hardness (mg/L CaCO,) Total Residual Chlorine (ml!.fl ) Temperature (°C) TVA /Sequoyah Nuclear Plant, Outfall 101, Non-treated September 10-17, 2024 Dav0 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Dav 1 Day2 Dav3 Day4 Initial Final Initial Final Initial Final Initial Final Initial 7.75 7.23 7.49 7.28 7.32 7.23 7.62 7.38 7.43 7.7 7.6 7.8 7.8 7.8 7.6 7.7 7.9 7.7 298 330 318 304 316 64 64 64 87 89 87 24.9 25.2 24.9 25.2 24.8 25.1 24.9 25.3 24.8 7.50 7.23 7.27 7.28 7.30 7.21 7.27 7.38 7.36 7.7 7.6 7.8 7.8 7.7 7.6 7.7 7.9 7.7 287 309 302 289 292 25.0 25.2 25.0 25.0 24.9 25.1 24.9 25.0 24.9 7.46 7.26 7.28 7.31 7.32 7.20 7.28 7.38 7.38 7.7 7.6 7.8 7.9 7.7 7.6 7.8 7.9 7.8 265 290 284 270 274 25.0 25.2 25.0 25.0 25.0 25.3 25.0 25.2 25.0 7.46 7.28 7.28 7.32 7.32 7.19 7.26 7.38 7.36 7.8 7.6 7.8 7.9 7.7 7.6 7.8 7.9 7.8 227 248 242 233 233 25.0 25.2 25.0 25.2 25.0 25.0 25.0 25.2 25.0 7.46 7.28 7.29 7.32 7.32 7.19 7.25 7.37 7.35 7.9 7.7 7.8 7.9 7.8 7.6 7.9 7.9 7.9 210 229 224 218 215 25.0 25.0 25,0 25.2 25.0 25.3 25.0 25.0 25.0 7.46 7.29 7.30 7.33 7.32 7.19 7.22 7.37 7.34 8.0 7.8 7.7 8.0 7.9 7.7 7.9 7.. 9 7.9 188 210 200 197 200 78 74 74 87 83 83 <0.10 <0.10 <0.10 25.0 25.1 25.0 25.2 25.0 25.3 25.0 25,0 25:0 7.47 7.28 7.30 7.34 7.33 7.17 7.24 7.37 7.34 8.1 7.8 7.7 8.0 8.0 7.7 7.9 7.9 7.9 189 207 198 197 196 78 74 74 85 81 79 <0.10 <0.10 <0.10 25.0 25.0 25.0 25.2 25.0 25.3 25.0 25.0 25.0 Project number: 19962 Dav5 Day6 Final Initial Final Initial Final 7.43 7.61 7.61 7.31 7.40 7.9 7.8 7.9 7.6 7.9 309 310 25.2 24.9 25.2 24.8 25.1 7.43 7.47 7.59 7.63 7.39 7.9 7.7 7.9 7.7 7.8 297 300 25.2 24.9 25.0 24.9 25.4 7.43 7.48 7.59 7.62 7.39 7.9 7.8 8.0 7.7 7.8 277 284 25.2 25.0 25.3 24.9 25.2 7.44 7.46 7.60 7.62 7.38 7.9 7.9 7.9 7.7 7.8 238 239 25.4 25.0 25.2 25.0 25.3 7.45 7.45 7.60 7.61 7.39 7.8 7.9 7.9 7.7 7.9 221 221 25.1 25.0 25.2 25.0 25.3 7.45 7.45 7.62 7.61 7.38 7.8 7.9 7.9 7.8 7.9 203 203 25.4 25.0 25.2 25.0 25.0 7.45 7.46 7.61 7.62 7_40 7.8 7.9 7.9 7.8 8.0 202 204 25.2 25.0 25.0 25.0 25.2 Entered by: J. Sumner Page 51 of 93

Entered by: J. Perez Reviewed by: J. Sumner I I I I I ("'"'* """'""uttc:llltvfs.1~.. k Page S 1.- Page_\\ _ of_\\ _ Total Residual Chlorine (4500-CI G-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L Analyst I 't-1/-K-Test;nltiation date cP,..., *.,_ -I I DPD: 11NR n.. -\\~ Samplos are anal'fl:ed as they arc received In the laboratory. The tut Initiation date Indicates the date the sample ls analyted for to(icltv. Positive and Negative Control: Control Type Sample ID Result() Positive Negative Negative control Deionized water Positive control Tap water .._/ Sample screening: Sample Sample ID Sample characteristics Result() number (color - clarity - part1cles - odor) Positive Negative ~,.. " -~ \\ E~~AN-Q.\\J fA\\L '"°'N, {..\\.f.A<t._ ~MLT\\t.~~ .._/ \\, "1..-\\a~cA-II"\\- C.T~t~.P~e. ~P.w c.. \\.eA.t () l\\(l.,l (_\\£ ~ .,,..~1)0..c,'\\. ~,\\JA \\ C;:,~ 10 \\ PA\\.t. Tj:).tJ C... ~.i). /( {1,..R..,,c..\\£." 7>W\\6~-o--\\ i lt->'1'-'~e PP.\\.~,,,._..., f, n~,il fPl-lt...t\\t L.e..~ -,.Jo0\\11:>. l\\o ..,-vi4.\\ w!>t.i,o \\ PA \\..e_,~I,:) t * <>A.Ill t ' ,..'-\\Dl'\\~S1 1N1"~~(. f>~, C>,A'-l f, P.A~ ~ Pl'lt**n t.\\.e; 61'l.Mt-.~ WW"rf' \\ ..__,,./ 1-.'-l o'\\ 10. b \\ t !,;;, r f)ed, k,.A- //,.:,,:,./" ,Oc,,/t-,' c/e--<:: r..'-\\0~1t>, 0-\\ N. \\.u\\U(~t,oro p6 /-d 7?<A-- c?:-o./ ..Ao/:r,~*S / / "'\\~Dl\\.1'1:1.c11 Mo~ /\\ft!:J (o(or cre().r ()(}..r+ :cres .. -to~10. o(. ))~'"'"J P'o,le_ f-41'7~ c l~r, 1 p11Vfrclef' v -.J oil\\ rt>. of r..\\liotow1w,'T'\\ fq fe 1'<1, cle'tr, Pcirf,c/~5 V 1,'-\\.n'\\IO. o'\\ -S A.l~~'1 'u..e. r)O Co(or., cieqr 1-'10~10.10 <w~~-4 V1o color, cfeo.r, pqr--ficleS ~ l Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. A positive result indicates the presence of total residual chlorine above detection (> 0.10 mg/L), which results in a pink to red color change with the addition of the OPD Indicator. A negative result Indicates the absence of total residual chlorine, which results In no color change with the addition of tt,e DPD indicator. If a color change cannot be determined due to the Initial sample color, then the probe method must be performed. tA Reviewed by I ~ Date reviewed ~===0~"1~10~*=U=?\\====~ SOP CB-Revision 8 - Exhibit C8,1 Page 52 of 93

Entered by: J. Perez Reviewed by: J. Sumner I I I I I I I I I I I I I Page l""'\\ Page_\\_ of_\\_ Total Residual Chlorine {ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL = 0.05 mg/L, Meter: Accumet Model AB250, SN 92349123 Analyst IJ11C, fJ: Date analyzed o'\\*,~- '\\.-1. Iodide reagent: INR IZ6'1 Acid reagent: INR 12.'i 3 Calibration: 0.05 mg/L 0.50 mg/L Slope Values (mV} (suggested range = 26 to 30 mV) Reference standard-# INSS~O INSS zz.,o 7-.'1,0 Note: For samples with a residual chlorine of> 0.50 mg/L. the samples must be diluted to be within the calibration range. Laboratory control sample: Reference standard True value (lV) Measured value (MV) % RS :: MV / TV x100 number (mg/l) (mg/l) (acceptable range = 90 to 110%) INSS 22.60 0.25 0.268 (O '1. 2. o/c, Duplicate sample precision: Sample number Sample ID Total residual chlorine %RPO:: {(S

• D) /[(S+0)/21} x 100 (mg/L)

{acceptable range=+/- 10%) ,'\\o'\\. ~ ,-1"' \\ ~~t-l \\C) \\ s 0,00 I .\\. Duplicate D 0.001 -711c O'f-ltJ *Z-'1 Sample measurements: Sample Sample ID Total residual chlorine number (mg/L) TV< 0.025 mg/L Method Blank (MB) o.Ot71 ""~ 00\\.b'\\.o'-\\ ,-.I~\\ St._~ \\~S:O.'£.CL 0- 001 "l.-\\.o°' ~o, *~ ,-l ~ I u.::iti.t-l \\o \\ u,oo*-s ""L-ll!l'\\10. \\\\ l,' lw~l

0. 00 6 -

Note: All samples were analyzed* In e~cess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control sample: Reference standard True value (TV) Measured value (MV) 'Yo RS:: MV / TV x 100 number (mg/l) (mg/L) (acceptable range= 90 to 110%) INSS t.z.,o 0.25 0.'2.'13 to-..1 Reviewed by I ,n -,n----ut Date reviewed I O'\\! 10* 1.<( SOP CS-Revision 8-Exhibit C8.2 I ~ ' o~**** Page 53 of 93

Entered by: J. Perez Reviewed by: J. Sumner I I I I f l~mtnulfcuii,vWilliotl&.lnt. Page Sl, Page_\\_of \\ Total Residual Chlorine (4500-CI G-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL::: 0.10 mg/L Test initiati:~:l::: I ~{~~\\".,:I I samples are analyzed** they arereteived In the laboratory. DPD: [ INR j 2.lj l,/ '-* _.:;..I.I"'_* "_'1__, The rut initiation date inditatu th* date the nm pie is analyzed for toKkltV, Positive and Negative Control: Control Type Sample ID Result() Positive Negative Negative control Deionized water Positive control Tap water v Sample screening: Sample Sample ID Sample characteristics Result() number (color - clarity - particles - odor) Positive Negative 2.iAcYI I l ' I I, EA~t "'~ - <<.w PrJ.lf -(0..T\\,cr~<J...' {h.r-1: cre( '2-Ll~ \\).* \\ 9 L I / tff~tS {i)t,(f -/-nn <.JP.r1 r, ffiO ~ere<; 1,);\\';Q I I,,*1 h ""'(r., \\ ~Q...N\\O\\ (' 0.. le,.. -to.'ri I c le,~ ( ()01+: (/f ( ,./ iuo~ 1 ).:J1 ~ 1-.ni\\\\((.. fO?...(p l / A, ..,/ {Qh I r.reo.. ' '(), l'frC(tf 2-1.{.:JUz,.o7 T~Al~-.l \\0 I P"le /q,1/ cf el.fl" I pqrf ~te.s -iito9 tJ. _09::, .1,* l~n\\~ P~lt. fq,t J C/9:tr i pQrl-icles 2-ll.Dqf2.0J Ul..A~ ~'"' vJ~ P q te ~ , c feq.r llwQ1~. oL( N. \\u\\ ~t,04l() Pale. fcl11, cle~r".. (lctrflc le5 ). u ;:;~/ 2. -I 0 ...t,),.,)fl nP pq/e. +c,n, cl6vdy, r;-rrf:ctes 1LfoQ /l.a9 ~ \\ \\( \\..., pq/e 'fqAI clear~' fq,f-;c/es J-li vq I 2. I I Ct.l Fl ( 11 ~> t,,)\\ r\\ Pale-fc111 J c.(ear '2,LJ.oO(z.11._ :::r ~~~tJ..J\\ue. Pct le -fq", cf ea.r 2, L! o q l 2, I S C..we,~ Wo C'~/or--,; c.louJy J P"r+ideS ./ 2 40912.tl} C..1~e.M,~~~ Pct le -fq,,, clear pqrfr c f~s 2 q oq/2. 15 ,J~\\\\CCO()\\ T41' 1 C lovdy I p~r+icl~s 20.oq t 2,, l l, l,.)~\\.L /Jo color I c(ea.r, pqrHcl~s / 'Pd Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. A positive result Indicates the presence of total residual chlorine above detection(> 0.10 mg/l), which results In a pink to red color change with the addition of the DPD indicator. A negative result indicates the absence of total residual chlorine, which results in no color change with the addition of the DPD indicator. If a color change cannot be determined due to the Initial sample color, then the probe (Tiethod (TIU St be performed. Reviewed by I 1{ Date reviewed 1-l~===a~(:..:,'.'\\c=-*=--~.=;..:\\=~ SOP CB-Revision 8 - Exhibit C8.l Page 54 of 93

Entered by: J. Perez Reviewed by: J. Sumner I ' -*----~ Page \\ & Page_\\_of_\\_ Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent To><icity Samples Matrix; Water, RL = 0.05 mg/L, Meter: Accumet Model ABZSO, SN 92349123 Analyst I ",v1 ?f Date analyzed !9-( / -& Calibration: Iodide reagent: I INR / 21, 'I Acid reagent:. IN R I 1, if 3 0,05 mg/L O.SOmg/l Slope Values (mV) (suggested range = 26 10 30 mV) Reference standard # INSS 'Z 1-6 o INSS 2 z_f, O 7-9, 'L Note: For samples w,th-a residual chlorine of> 050 mg/L, the samples must be diluted to be within the calibration range. Laboratory control sample: Reference standard True value (TV) Measured value (MV) 3/4 RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) INSS 1,,-'2-6b 0.25 f ). 2-£"~ ll'Js. 2% Duplicate sample precision: Sample number Sample ID Total residual chlorine %RPO= {(S

• D) /[(S+0)/21} x 100 (mg/L)

(acceptable range= t 103/4) 1--~\\ <7 q JI o i I,M~t:,ou, 5 f!J,f~/ -~ Duplicate D @,L79 r, ~~- ll. /~. Sample measurements: Sample Sample ID Total residual chlorine number (mg/l) TV<O.OZS mg/L Method Blank (MB) ~.&o5

• 1-~ t?q \\ \\ J3h T..Jf-.. \\ ~fi:):.N \\0 \\

0.009 'I t,\\l)CHJ,}1 \\., ' l~A.~t. g nntf i ~-.I? } JJ Note: All samples were analyzed In excess of EPA recommended holding time (15 minutes) unless otherwise noted. r Laboratory control sample: Reference standard True value {TV) Measured value (MV) 3/4 RS : MV / TV x 100 number (mg/L} (mg/L} (a~ceptable range = 90 to 110%) INSS

• LZ-6rJ 0.25

(!). 7.~5 -.I}-,.., /!}'...

• r,qo_,.,,:

Reviewed by I I Date reviewed I a'\\*11-"'L-' SOP CB-Revision 8-Exhibit CB.2 Page 55 of 93

Entered by: J. Perez Reviewed by: J. Sumner I I I I I I I I I I I I Page S°'1 Page _\\_ of __ \\ _ Total Residual Chlorine (4500-CI G-2011), Screening Whole Effluent Toxicity Samples Matri)(: Water, RL = 0.10 mg/L Analyst !l<"l ~ -~-----, Test initiation date Sampl'es are *n*IVl*d as lh*v are reuived ln the laboratory. .__~_#\\_, _1~_*_'\\:_~_~ The te.st initiation date Indicate, the date the sample is analyted for to~icity. DPD: I INR 17-l/. 4 Positive and Negative Control: Control Type Sample ID Result () Positive Negative Negative control Deionized water Positive control Tap water Sample screening: Sample Sample ID Sample characteristics Result () "umber (color - clarity-particles - odor) Positive Negative Z~t:, *7.:1 E:1'.!:i.~"'"~ (lu.; lf&J.,-r,rv,f~./-a,,,,.. //,pr, D,.-,/',1--' ~*fe? ./ t / / / v &l{O~I ~. L,j E=~\\.v~ / f.eth;- J)r_d,,.. k./L _ /' /'&>,,.J' }?; t-t;~,,t:> <,. 2.411Q 11 26,.J"',~ \\I)\\ ~q,~

• oi11, c,r(J~r, ~ r+L'c~s

'l.,0\\o9 11-21 .\\ ' I ~1"1\\°(.(. lode +n 11 1 C{po, r, fJa..f+CC 1ef V"" -z.~l aow\\. oS '"" \\ ~~~,o, Pct le ~ 'c le'\\,- -,..1 n'\\,'-t.tib "~. \\.,;), ~\\(.(_ P C)(,1-e ~ / cfl?q,r-1 pc,,rftc/eS'" ';,;t.~* ~G <t.A.~M,\\ I.U~ ~- ,_4 04'. ~"'- /,,/5,h/- ['Ju(P,.!z:,,,,L, t! f~ "'L,-\\bO\\~~ i,,A-"'"- t...\\, w,~~oto ~~te wii*,,crer:Ar,fur..i-tcter / -,.J nA1'-\\. (fl.. t--\\01-d.De.. P*te 1lfr/. c /.p,a,,' (Jq.tl-! c-1es I -z__--tnc.,,.. n\\ ~\\~\\~ pqf.e,I.e.,,,I e,(~ 'pt:tr--ll~~ V /" "2.,~ n, 1\\l 11' u -\\Q t o U,) )t-l 11'i 1 fJ<f !e..few,, c 1 (p/. ~

JC(ff

1. 0 4, II.\\. t.4

T AU($t\\Jv1u.e

'r"'k r,m, c~' J ~~~""'t ~ ~ ~ Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. A positive result indicates the presence of total residual chlorjne above detection (> 0.10 mg/L), which results in a pink to red color change with the addition of the DPD indicator. A negative result Indicates the absence of total residual chlorine, which results in no color change with the addition of the OPO indicator. If a color change cannot be determined due to the Initial sample color, then the probe method rnust be performed. t,O..* 0,. 1 Reviewed by I 8 Date reviewed ~===;;ii;:::.~,'\\=*=b\\====~ SOP C8-.Revision 8 - E)(hibit C8.1 Page 56 of 93

Entered by: J. Perez Reviewed by: J. Sumner I I I I I I I I I I I I I I I I <0 Page __ _ Page_i_of_*1_ Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL = 0.05 mg/L, Meter: Accumet Model AB250, SN 92349123 Analyst I 1q Date analyzed --~ -_..... ,_o:=9*-=13=-=--Z=l/~==: Iodide reagent: INR 1--------L---l Acid reagent; INR Calibration: 0,05 mg/L 0.50mg/L Slope Values (mV) (suggested range= 26 to 30 mV) Reference standard # INSS 2 100 INSS 2 '2 t,O A9,"1 Note: For samples with a residual chlorfne of> 0.50 mg/L, the samples must be diluted to be within the calibration range, Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/l) (acceptable range = 90 to 110%) INSS 2 '200 0.25 0. 'Lt>v\\ t?S. c,~,. Duplicate sample precision: Sample number Sample ID Total residual chlorine %RPO = {(S

• O) /[(S+0)/21} x 100 (mg/L)

(acceptable range= :t 10%) '2..,U-;79 l~ * 'l 6 -rvA \\ $~,o \\ s o.oo 6 -V I Duplicate D

0. UD £...j fi(}&q. /°]-~/

Sample measurements: Sample Sample ID Total residual chlorine number (mg/l)

• TV< 0.025 mg/L Method Blank (MB) 0

, i? O 3 'A L\\o 913 , ?., \\\\J /1-. I <_Q ~ I loJTl\\\\(e. 0 Do '-I ./ ~ _,,,/' ~ 0</ 2<-/ Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number_ 11 o4~1-1L (mg/L) (mg/l ) (ateeptllble range = 90 to 110%) INSS -~ '-"'l. --5-1., *2'2 60 0.25 o.~60

Ol)..o'/,

Reviewed by l ___..td+\\-_. Date reviewed I cA*\\'\\*"\\.-\\ SOP CS-Revision 8-Exhibit C8.2 Page 57 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page _o?Cf.:......,___ Page --1..___of 4 Alkalinity {SM 2320 B-2021) Analyst l &c.. Date analyzed oq. \\'5.'2.'-4 Matrix: Water, RL = 5.0 mg CaCO1/L, Samples are titrated to pH= 4.5 S.U. pH Meter: AtcumetModel AR20, SN 93312452 Titrant normality and multiplier determination: Tltrant reference Normality check Begin End Total Normality (N} of H,so. pH Factor or Multiplier number standard number ml ml ml = (5 ml Na,CO, x 0.05)/E = 0.25/E = (Nx 50000)/ 100 ml sample (E) (acceptable range = 0.0180 - 0.0220) =NxS0O INR,~~1-INSS ~~&JC\\ D.C> 11.i 11.i Oo.;l1~ le. 1,p Laboratory control standard (LCS): Reference standard True value (TV) Sample Begin End Total Multiplier Alkalinity (MV) % RS =MV /TVx 100 number (mg CaCO,/l) volume ml (ml) ml ml (mg CaCO,/l) (acceptable range = 90 to 110%} INSS ;);i. \\:> 100 100 I I.cg, ,h'S '\\.1 10.\\o )~~.elo:3 ID3~/o Duplicate sample precision: Sample Alkalinity %RPO Sample Sample ID volume Begin End Total Multiplier (mg CaCO,/l) = (IS

• D) /((S+D)/21) x 100 number

{ml) ml ml ml {acceptable range=+/- 10%) 01.12..2.~ C rnHsi,J IOI) .;;>\\.5 .71.4

5. '}

10. \\.,

s \\,.J ~ Duplicate (D) j_ ,;n.~ 33.~ 5.q ..L D ~.:2 Bs\\. - Cfl,t,,1.~ Sample measurements: Sample Begin End Total Multiplier Alkalinity Sample number Sample ID volume (ml) ml ml ml (mg CaCO,/l) MB (TV< 2.5 mg/L) Deionized water, pH =4.'-¥1/2 s.u. 100 0.'0 C), 1:> C,v 10,IP i-.) 0 01.12-2J1 H mHsw 100 .2;z,, l. 31.~ (q.o "'-i.f Oj. \\2-2.'-'t 6 2-Ct..li ~S.3 (.,.O Le+ IAQfo.:2 y o.o ll. o \\.9.o u+ t) 0 -~.~~ ~.o ~ l.P.o le. 14-o B-a1.o"1Y fl "~ l~.o ~-0 ~..... (Jj,;). '1-~ 8 l~.o ~'+ 0 ~:o lo4-ot:;.04,i 4 ff ol4.o 00.. 0 ~.o lt4 01.u~*~'-4 b ao.o -~.o ~.o le.4 !M.4-4_,u c.. ~ .o ~.o l.,,.o ~4 ~-D~-1'1 l) £4~.o 4~0 l,.-o lo4 ~.tA.,-i./ A O.o t,.o l..o L.4 rJ6ibq -~~B la.-=> IJ.o l,.~ (Q4 04.~-!l.LI /} 5A-t%' 5w l~.o oho 'to q5 01.o-1,i., B ~l.o ,3,:,,'5 '\\ '5 loo O"J. *O-~~ ~,':> ~lf.'l ~-4 iq (/il.t:>,*i\\./ 39."I '+ti.& 'L'l ioo o'\\-o-4*'\\.-\\ A rnlh.iu ou l 0.0 l,. o lg_,=> Li'+ ,..,.e;A-'\\,~ \\'.:) ( 2-u.o 1.;i.c ~-... 44 () 6\\*01\\* 1.'-t ~ j .J _i.....- l~-o l'&.O l.i.o LD4-Laboratory control standard (LCS): ~standard True value (TV) Sample Begin End Total Multiplier Alkalinity (MV) % RS = MV / TV M 100 number ml ml (mg CaCO,/L) (acceptable range = 90 to 110%) l'**o (ml) INSS 100 100 12.-.,L 1~'1,C1 Reviewed by: Date reviewed: I 07(-t~-l'-( Page 58 of 93

Entered by: J. Perez Reviewed by: J. Sumner l I j I I I

• =

Analyst ~-..£6 ---t Date analyzed c;15.24 Page :3a Page -=-{ of '-+ ~-- Alkalinity (SM 2320 B-2021} Matrix: Water, RL = 5.0 mg CaCO3/L, Samples are titrated to pH= 4.5.S.U. pH Meter: Accumet Model AR20, SN 93312452 Titrant normality and multiplier determination: ll1.,a11 re *._, .L.L Begin number standard number ml INR INSS Laboratory control standard (LCS): ReJerence standard True value (TV) Sample number (mg CaCO,/l) volume (ml) INSS ~~\\~ 100 100 Duplicate sample precision; Sample Sample f1' JA Sample ID volume number (ml) -,..ino._°".~ 5a),-J f 01 I 5o 1 Duplicate (D) l Sample measurements: Sample number Sample ID j ~ l j .3 111 ff.Sw Anh* I 'l. J 3 ' I J t.. Laboratorv control *

• ~ ILCSJ:

End Total ml (E) Begin End ml ml l~.o .n.u Begin End ml ml ~;}I, 31.~ 31:3 35.:> Sample volume (ml) s.u. l.VV I .L S'o Reference standard number True value (TV) Sample 1>e.,. (mg CaCO,/l) volume ml ml {ml) INSS 100 100 Normality (N) of H,SO, pH Factor or Multiplier - I~ ml Na,CO, x 0.05)/E : 0.25/E - {N x 50000)/ 100 ml sample (acceptable ran11e: - v, AA n "220) = Nx 500 - I?,~ oit IS-2.4 Total Multiplier Alkalinity {MV) % RS = MV / TV x 100 ml (mg CaCO,/l) (acceptable range= 90 to 110% '\\.4 )o.w 10,< IC~.o0 fo Alkalinity %RPO Total Multiplier (mg CaCO,/l) = {(S

• D) /[(S+D)/21) 11 100 ml (acceptable range= ;t 10%)

10.lp s ~ 3.1- ( ) ..l D

i&

e6L-o,

• IS°*-1-,

3.1-t :,) Begin ml End ml Total Multiplier Alkalinity (mg CaCO,/L) 35.0 o.o 3.'5 Tntal ml ml 10.-,.. 3.1- (>\\ 3S c :n lo4 91 Multiplier Alkalinity (MV) % RS= MV /TV>< 100 ~ (acceptable range= 90 to 110o/. Reviewed by: vL Date reviewed: I O'j.1 lo*~ 1.-l 'k Q,11. oq. \\'5. 2.4. rt~,). Ji!{: ~ }-k, w n"j

• Page 59 of 93

Entered by: J. Perez Reviewed by: J. Sumner Analyst 18$(... Date analyzed O'\\. 15,2. '-t Page.31 Page,l of_'-+-'--_ Alkalinity (SM 2320 8-2021) Matrix: Water, Rl: 5.0 mg CaCQ3/L, Samples are titrated to pH= 4.5 S.U. pH Meter: Accumet Model AR20, SN 93312452 Titrant normality and multiplier determination: check Begin End Total Normality (N) of H1SO

• pH Factor or Multlpller number standard number ml

m.

tr; t Na1CO, x 0.05)/E = 0.25/E = (N x 50000)/ 100 mt sample (E) (acceptable r ft n, 80

• 0.0220)

=Nxsoo INR INSS ---~ Sx.. 0Q.1c;.2--1 Laboratory control standard (LCS): Reference standard True value (TV) Sample Begin End Total Multlpller Alkalinity (MV) % RS = MV / TV x 100 number (mg CaCO,/t) volume mt mt ml (mg CaCO,/l) (acceptable range = 90 to 110%) (ml) INSS ol=i 13 100 100 ~\\.'5 a1.o 't_L:, 10,1, I DI I cl.o'IP Duplicate sample precision: Sample Alkalinity %RPO Sample Ci'Jt~~~IO volume Begin End Total M ultip1ler (mg CaCO,/L) = {(S

• 0) /((S+D)/ZI} x 100 number (ml) mt ml ml (acceptable range = t 10%)

-,-_~ n41\\.\\~ '.l..'- t+nJ.: 3 60 31.0 35.4 -4.4 l l) IO I,,, s q3 l, Duplicate (D) ..L 36.4 lti .-+.4 ( .l D 8Sl. - O'f./~-2"- ~) q~ Sample measurements: Sample Begin End Total Multlpller Alkalinity Sample number Sample ID volume (ml) ml ml ml (mg CaC01/l) MA.ln/,c_-, C --" I nolnr,jzed water pH = ,~~ '°5L oq,15.2_-.. "IJ11~. C \\ ~ A d,*c14n R W } 5c o-'ii i-+3.4 3 la { :>\\ IO.,b -::i I., 1..-.\\ 0"'1 I. 1 1-I '2. 4~.'+ "+1.~ 4,0j ~\\ i5 '1. '\\ rA ~ ~,. ""\\_ I J 0,0 '"i.2 4.2,. {.) } ~q '"1.-'rAOO\\.O\\ lruJ..rn.t;.ri P-1 JA-n 1,* I 1-i. l- 'i 0 ~.i ' )} i\\ ,.'\\~}_\\. \\ "'

z_

i.O l~.o -+.-=>> ( ) ~u-->l ~(0 ~ r:A \\""i,. '\\, "\\.-- .2J 1a.... ll,. 2. 4-, -Z. I \\ ~cc .,__~,__,\\..( (1 lf"l ~-"'ha cLt. )o~ ,~.2, 1'-=l*\\,<> 1.-+ 15 "l.4o'\\ll., 0 -Ptnll.v j_ \\-=l,v ~l.1.P ~.o ~ "l..40'\\l '-* I<; -fA ~le.. nm ~'5 ~I.I,.;n.s s., ("-l) ~o ,... l0'\\11..1\\. l ulJ//L So 9'1-6 ~9.lP ~. l l ) 'il-t "l 4Dl\\lt>, 0 \\ t:->iuh t:vr> l \\o-o ~<'f,1, 3;,.i

5. 2.

'5"- ,~d\\n.a\\ 1.- \\ ~4.q 'ii.'l-lP.4-1ai 'l._'-4 o'\\ l ~, 1.;; \\ .3J J.- 4\\.1.. ~., 5.1-L,o 1.'4 rl\\J I). 0 ~ t-l lua I k~ orw \\ 100 oo \\.'fl }. i '°' \\.... d\\l"l..o...\\ 2-I I. r< .3.'} J.t

J~

.,_4 o4\\,~ * ~-\\ l J J_ ,3.q S.1., 1.-=t \\'3 -i'-'\\ O'\\ 10. 0~ D/\\,'k,A.\\ Q-i I J5

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~-" 3.;,... ) )40 '"'\\."\\ b"\\,,. *"" I.S r lot+ ( 'l-. I i.C\\ 10,4 .) -,_ "'-..ct\\ 1-.\\.

• b \\

J .3 I \\0,'+ 11.s l.'4 l ~) sq Laboratory control standard (LCS}: JIVI Sample Begin End Total Multiplier Alkalinity (MV) % RS :: MV / TV x 100 number (mg CaCO,/l) volume ml ml (mg CaCO,/l) (acceptable range = 90 to UO") (ml) INS5 100 100 - BS1. otf

• l"i,H Reviewed by:

Date reviewed: I J 1 *~tp.1. y Page 60 of 93

Entered by: J. Perez Reviewed by: J. Sumner I I I I Page 32-Page 14 of_-4...,__ Alkalinity {SM 2320 B-2021) Analyst I es~ Date analyzed 1--0"""'i-, -,s-.-2-4-----1 Matrix: Water, RL = 5.0 mg CaCQ3/L, Samples are titrated to pH= 4.5 S.U. pH Meter: Accumet Model AR20, SN 93312452 Titrant normality and multiplier determination: Titrant reference End Total Normality (N) of H2SO. pH Factor or Multiplier number standard number ml ml I<. ml Na2CO3 X 0.0S)/E = 0.2S/E = (N x 50000)/ 100 ml sample (E) (acceptable range =.... n 0120) =Nxsoo INR INSS ea cq.~*2.J Laboratory control standard (LCS}: Reference standard True value (TV) Sample Begin End Total Multiplier Alkalinity (MV) % RS= MV / TV x 100 number (mg CaCO3/l) volume ml ml ml (mg CaCO,/L) (acceptable range = 90 to 110, (ml) INSS ~\\:l;, 100 100 I 1.'b ~\\.I 9,3 It>. Ip '1 'l qG\\,o'l-> Duplicate sample precision: Sample Alkalinity %RPD Sample Sample 10 volume Begin End Total Multiplier (mg CaCO,/L) = ((5

• 0) / ((S+D)/21) x 100 number IY) h"'I ' l}l,.,

(ml} ml ml ml (acceptable range=+/- 10%) 1-'-I 0"11 o,<f'\\ I loo ~).\\ .J~.'t 1.3 l c:,, 'iO s 14 l Duplicate (D) j_ ~~ -1+ ~3-t I. 3 j... 0 14 l?,'Sc..,. -()').11~ Sample measurements: Sample Begin End Total Multiplier Alkalinity Sample number Sample ID volume (ml) ml ml ml (mg CaCO,/l ) ,...-,o 1.v < L.S mtstLJ - *-***---.... a11.111;,,.,, = __ s,u. J.VV e61., oq,JSN '1.'°4'0\\11..10 fr\\ /tr\\/)\\_"'

l.

loo .:?3.~ ~4.i l.1 \\o.v I~ "l.-\\d\\1'-'.01- \\ J J ~4.'! ~1#.o,.~ ,~ 1.J ""In. I\\,, rf JlA tnRJ\\J I O I i 5'o .;; '-,,,0 31.'.l 5,2, j ~) 110 ,_ 4 00\\ rt.. U'\\ I '}.. .31."- 6!J5 c,_~ r :n 110 -ltz.. "1.--\\ b~ I~.O'.::, J .:) 3~!5 '+\\.'\\ 5.4 t l'I. \\ \\ \\() -11'-'L "\\oJ... a.,..... \\, 1/\\JA W~N lui,~-r 1 >-\\-1. t\\ 4/t.O Lf.1 \\.p,) i'1- "\\.\\l rA \\,

• r-."

J "1.. O,o 4.1 (. ~) ~'1- "l.,°'4. "'" '""'

• ft(,.

~ j ~. I 1.1-4., I,\\ '6'1- -...I ti'\\ In. 1)1' Cflo Wu) nik I ~<:> 'B, 1,., \\j. \\ 4-,q l~ \\ dl-o "l.-1/2D\\ I,_,\\\\ I 7,,, I l';, I lg."'2. S. 1 (,,) ~1-o "}\\.I.... 14'- ~ ,.J .J.. \\'6,1, c23.; 5,1 {!'f) c<~ ..,, "'",,.,,o"\\ Jl\\u..~ \\J' \\ u l s ..?3.. ~ as,; S.o L~,\\ I IC::O .,. -~ AA 1,. 11-I 1... I ~'6.2> 33.°I 5.v 1.: ~\\ l~~o 'hi A4' I \\of

• 0 L.{

J _ji .J_ a.3.'1 ger.o 5.1 l 10) I lar-.. "l. "\\1)1\\ \\ \\, '!)'-\\ 1Y1 inu. hn, tJl ~5 3-,.0 Lf.,+, \\, 5,Cn I 14) _l- ~L-0

,,. 1 IU__fV.U j

I z J __j---- ~ -L-----":= SSL. O'H5*l'+ Laboratory control standard (LCS}: Reference standard True value (TV) Sample Begin End Total Multiplier Alkalinity (MV) % RS: MV / TV x 100 number (mg CaCO,/l) volume ml ml ml (mg CaCO,/L} (acceptable range= 90 to 1101 (ml) INSS ~a,~ 100 100 ol4.~ ~?-1 9,4 \\(),\\,D 100 I oo.o't>I-> Reviewed by: Date reviewed: I O'i*1la-J'-I r

• I Page 61 of 93

Entered by: J. Perez Reviewed by: J. Sumner I I l l I I A Analyst I ~k Date analyzed (L. I-!: :>,l Titrant normality and multiplier determination: Titrant reference Normality check Begin End Total number standard number ml ml ml (E) INR...,.-:_,_~d../.... " INSS.;:l~Si 0.0 q,, 9.~ Laboratory control st't:,"r1i1ard (LCS): Reference standard True value (lV) Sample Begin End number (mg CaCO,/l ) volume ml ml (ml) INSS ~.:J.:;i 5 40 so ct," H.\\ Duplfcate sample precision: Sample Sample Sample ID volume Begin End number {ml) ml ml 'J_..,_,.)"J.7 UNw\\n t.~ 5o ,,.i,~." ) Duplicate (D} J_ ~.c, 1'-4-. 0 Sample measurements: Sample Sample number Sample ID volume (ml) MB (TV < 2.5 mg/l) Deionized water so -Z.S"302-3 I }t,J W\\/"'\\. {J l--:;?ict20 (}.)Cu-( ()Jf P P JI "- t,~ l<i vi I Jn '2-s~v, (J)~\\c.l,a QJj z.;30<.or j I U.o ~ {'f..AV\\ .f'th I 6o 12~ R.~ e.Jh Jn ~ t,(') 50 FB f-,1y.,,.m, ' lh.J () I 0\\:1-4 rn 11 /4 11\\lJ I O<\\~t-1 ~ tR l1I a"ioq ~JP D1"" JO,=: ~P f-,t; 'Ju LI f Wd) b e~£r1i\\f t'hlt61.-0 <.t> *J.e..\\~ O~-.,\\t:J*l4 R c,eJ$J-a4 B I Laboratory control standard (LCS): Reference

• • -**--*---.... True value {lV)

Sample Begin End number {mg

• • -e ml ml (ml)

INSS 40 so Reviewed by: Page Hardness (SM 2340 C-2021) Matrix: Water, RL = 5.0 mg CaCO3/L Normality (NJ of EOTA pH Factor or Multiplier -= 0.2/E = (N x 50000)/ 50 ml sample (acceptable range = 0.0180 - 0.0220) =Nx 1000 0, 0.;l.o_'.2 .;Jc, ;;;i Total Multiplier Hardness {MV} % RS = MV / lV x 100 ml (mg CaCO,/l ) (acceptable range= 90 to 110%) I. q ~~.:.:l 3'a Cf 5. \\),. h-Hardness %RPO Total Multiplier (mg CaC01/l) = {(S

• 0) /l(S-+D)/2J> x 100 ml (acceptable range = t 10%)

I. I ~;,.~ s .Ja. I.I J. D olJ. 6SL -~* N-2-4 Begin End Total Multiplier Hardness ml ml ml (mg CaCO,/l) 0,0 O.o 0,0 ~ -:I.. t-JD l"t.b ~-" l'). 'I IS? I-+.'\\ ,~.3 b4 (i', I 15.~ 16\\.11 o.~ <D. t \\S"

Jo.v 5.o ICO

~~ - "1 ..q1,4 o.i 11.... .J1.-l. ~.J. "1/4-I.~ c,<(s, ~J."l l.'.>.:i..LI o.q ,~ .:/3." ~5.'i 1.'l '*~\\ I <<=JD ..?5.'5 .:lt, 4 0.9 I~ Ji.,"+ -31. l 1.1- ~&.I.. c2f. I << 'L'- J. a. a?--+ o<'l.~ di.~ .:/.o 4C> -~ I *'- 33.o 0.1 I~ .2..:>.o ~5 C,5 10 I.a.,.S 3:l ~ o_q 1g ~:z. 4-3 :1.'l .LI,. ~ 5i"l-Ji1 '1,. 11,il. I 4 -4 'ifq ~ -1 ~.ii 1-4.'5 91 D.O '-+. 3 '{; .. i.-- n Total Multiplier Hardness % RS =MV /lVx 100 ml (mg CaCO,/l) {acceptable range - 90 to 110%) -12.-........ 0'!.f-l* Date reviewed: I 01.\\-;.2,~ Page 62 of 93

Entered by: J. Perez Reviewed by: J. Sumner ,..,,.__..,.f>,......,...._.. 1<c. Analyst 11--B.c.:~=-1.,=--------1 Date analyzed O't. I'+* -<-4 \\ ntrant normality and multiplier determination: "'ormatitv check Begin End Total number standard number ml ,.. ~ (E) INR INSS Laboratory control standard {LCS}: Reference standard True value (TV) Sample Begin End number (mg CaCO,/l) volume ml ml {ml ) INSS 40 so 4.~ l4-.~ Duplicate sample precision: Sample Sample Sample ID volume Begin End number (ml) ml ml D 1,,~-~\\{ C tlh~w 5o u.J. \\o.~ I Duplicate (DI

l.

Io.':>,.... ~ Sample measurements: Sample Sample number Sample ID llolume (ml) MBl'TV~' ~ --.:'" -.

• "'ater so M. \\).~Lb~

()) IH, kJ 5o ffi.,-a -i'-{ & 0 q,,i.Li)i4 C) 01.~.a"< f3 ~ 064 ~'1:s,&--142 o,.~~4 n nq.,~.~ A ,1 'i. ~ '"i"i 6 Ill\\* "'4 *'--l A mH.sw UV I OC\\*~*'\\"'- C) I '2 00\\*CA* vl. e:, ...J 3 2 *I 6°'dD\\.t"l HOA~,J 11)1 l 50 "l.'-1 0 °'I\\. "\\,, I 2-1-.i 1\\0\\, ~. l.b J ,. -.I..... rA, O"'-\\ - 'vA ~d fol IJIJ I "Vt Ml\\ _ ~la I z ,... \\ d\\ \\~ 1t.. _J .,_.\\....... All.Al -1'1A~.t-.J1 ~'\\f I ,.,, I)~\\\\.~, _I z_ Laboratory control standard {LCS): Reference stanaara IM~ r - -** Beirin End number (mg CaC01/l) volume ml ml (ml) INSS 40 50 Reviewed by: Page _4_5_ Page _,,,.c2 __ of ~ / ( ~,~t,"'

• .;;,°

Hardness (SM 2340 C-2021) Matrix: Water, RL = 5.0 mg Ca(Q3/L Normality (N) of EDTA pH Factor or Multiplier = 0.2/E = (N K S0000)/ SO ml sample (acceptable r - A*an

• n 0220)

=Nx 1000 -~l 0-=t.1..l.~ Total Multiplier Hardness (MV) % RS =MV /TV x 100 ml (q,g CaCO,/l) {acceptable range= 90 to 110%) I."'\\ ~0.i -~c;c 'l"5.o,,.. Hardness %RPO Total Multiplier (mg CaCO,/l) = ((S

• D) /[(S+D)/2)} x 100 ml (acceptable range = +/- 10%)

4.~ o?o,do s ~1-J._ D +.; '3"+- eo'-' 0'\\. lil.'Z.~ Begin End Total Multiplier Hardness ml ml ml (mg CaCO,/L) a_..,,_ f)el,\\J. '.)J 14.~

19. I 4.~

o?o.~ ~1-l't I ~.3.'t H-.::a. i1- ~3.~ .:n,.,'J. .u.,:i. '&1- ~~:l- ~.o 4,-; ~'t -~.o 31.1.4 4.4 ~ ~... ~ >-lo~ '-+.~ icy .a.fo.'i ~-? 4-r.i. ~q O.c '-\\,~ 4.~ i'":l- "" ~ 8". t,, 4.:l. ~"} i,\\, Io. 2i "+.'1-9C.. I~.-; ll l l.4J. ~q I ':l.,._ I.J~.o 'S1- ~at.o o?1,. 1 'i-1 'a~ "".I ~"\\.~ ~ - I io .;-.,.~ :.l-"-"" "+. I <i;"l, .2-4,j, 3CZA* '4-. I j~ ~&A i'.l'i°-,-~ ~.'+ 4.0 ~\\ ~2..4 ~1,.1.., >+..:i. ~ o,.:, 4.o 4-.Q i1 Total Multiplier Hardness % RS = MV / TV K 100 mL ~CO,/L) (acceptable range = 90 to 110% - ~CYl-1J.* Date reviewed: I 0°>-1'5 2,--t Page 63 of 93

Entered by: J. Perez Reviewed by: J. Sumner \\ -*-- t.-.1, ...... W..wM..l,,1.. Analyst I 8-s<- Date analyzed O"I, 14 ly Titrant normality and multiplier determination: Begin End Total number standard number ml (E) INR INSS Laboratory control standard (LCS}: Reference standard True value {TV) Sample Begin End number (mg caco,/l) volume ml ml (ml) INSS c:.1..2.t~ 40 50 >-to 5q Duplicate sample precision: Sample Sample --r..J.A Sample ID volume Begin End number , (ml) ml ml ..,. J "°',-,,. 'l.. 1 l.36\\,JI kli' ~ 5:o 5, 'l 'i,'b l Duplicate (D) J_ 'L '5 )3,.1 Sample measurements: Sample Sample number Sample ID volume (ml)

.,_ \\Iv ' -'*

, mll{l/

fe1omze<1 water o-- 1....\\01\\01\\.IP!~ ~ vA-SG.,J I NI l)J } 5o -,...irA,\\~,! It'.. r

2.

,;1 ~"'" J _j 1-..inQ.1~ :a.ft,; l'0.*-1.*l.,~ 4 In ~v. 1 J'.:\\11 +: ' o~-~blt> I z O"\\* a-.-1.--\\ e:. I 3 -1.-- "\\..-.lnAM, o-, l/hhn£/,.-, l ~5 '1.--10~ \\). I~ I 1_ "l.~ o'\\~-,.~ I 'J .....i 11AIIA, trl -, !0-( hY7 tl"'1 ( -fu }..; l ,Joot1l.14' l '2. "1..-.l l'IA *'-,,_, J

7)

,.~""'~-0\\ I~ t'l-<hntV) ~1... 1 \\ -i. '-\\ o~, \\.\\ k I 1.. "1.~.. ~ \\"\\. "l :l... - l 3 -.Ji14'c,c:ii0\\ V3/4J-n~ Kw Anh. \\ 'l..'ib'i. \\\\ \\ '° I z.. ..,_'"' 0~,~.,. "\\.., J _} - "\\.'ici'*h'l.,t"\\ r.ru1n ho rJ.J 5o Laboratory control standard {LCS}: Ref-erence stanClara r---*- ft._,_ ~.. d number (mg CaCO,/l) volume ml ml (ml) INSS 40 so Reviewed by: Page J.+k, Page :3 of _4.,____ Hardness (SM 2340 C-2021) Matrix: Water, RL = 5.0 mg CaCQ3/L Normality (N) of EOTA pH Factor or Multiplier - 0.2/E - (N x S0000)/ SO ml sample (acceptable ran,.,. A A 1 an - 0.0220) -Nx1000 ~ O'l, lc.l"l.4 Total r,ilultlplier Hardness (MV) 1/4 RS = MV / TV x 100 ml (mg CaCOJ/l) (acceptable range= 90 to 110%) I. 9 -~~'l. 3i '\\'5. QO\\o Hardness %RPO Total Multiplier (mg CaCO,/l) = ((S

• D) /[(S+0)/2)) x 100 ml

{acceptable range=+/- 10%) 3.~ t:10.Q s +~ _l D -=l-C\\ ?,,<\\ e,sl, - cq. 14 z.4 Begin End Total Multiplier Hardness ml ml ml (mg CaCO,/l) &st., o.:,.i-lil~

• ~.1--

I ".t.<l -4, I ,<o.~ i°l:> 11-<l ~,.'ii ...... 'O I ~I ..? Li ~6.i 4.o f\\ ~5.& 3o.; 4.5 en -~0-~ ~\\ tf C, 'f I .1/4i 3"t,o 4,,. ~5 3(f.o 41.4 ~...., 'n --\\-\\ 4 'i~.i.?.4 ' I \\ '1'} 4~.!I l.-...11 *. 1 4.~ ( ~, 9.; D.o ~.'+ ~~L ~, q1,- .:J l.j. ,J.,'?> ., tJ. ( I!\\\\ q1- '4S -:J.,.:2 .:J..i.l ( h\\ C\\~ ":J; a 'L"1/2-ol.~ r h\\ 'i'l <=t.4 l\\. v

J 2 r,,

~q 11.~ l'-1-.o _;), ~ ( 1) i':}- J4t> \\ \\i ~ ~.~t 1\\ '?q \\v..1,~.-~ ~. I f,, gt:j \\1.~ ~.::,.\\, :i ~ u., q~ lJc,v oV+.'° ~.o ~, Total Multiplier Hardness % RS = MV / TV x 100 ml -- (mg CaCO,/l) (acceptable range = 90 to 110%) &1., O'l*1i14 (/C Date reviewed: I O'i lSfH Page 64 of 93

Entered by: J. Perez Reviewed by: J. Sumner I I I I I I I I I I l I / ,,...._o11o,.....,...._,.... Analyst I iisl. Date analyzed===O=C\\==*,=4-==*2==4==== Titrant normality and multiplier determination: Normality check Begin End Total number standard nu ml ml lCI INR INSS Laboratory control standard (LCS): Reference standard True value {TV) Sample Begin End number (mg CaCO,/l) volume ml ml (ml) INSS ol.~.15 40 so ..?4 '- du*IP Duplicate sample precision: Sample Sample Sample ID volume Begin End number (ml) ml ml 1..'1\\l'\\\\n.o\\ f!-.,ttJJhwn l 5\\) .:;,\\,," ~"l.v l Duplicate (D) j_ .2 '\\. \\I -37..\\. Sample measurements: Sample Sample number Sample ID volume (ml) A.AD,_ .,,,. --, C ... ~*-* c;n U.l l'\\~\\~-0\\ 6;, an a /l"'"\\

1.

5o , J,~o.,,._,c.. j

J J.-

,J,...,,., b-..\\ "\\-l l1 \\,\\ k~)~ \\ So 1.4 o0.1"\\.. 0...\\ I 'Z. I '1.-.t o"'\\ r,. \\.~ J .3 1..1.\\041\\ \\\\. 10 Vll\\dll ~-\\c)'\\rl.. t; hVA U't 7l'.> I 10 ,. '-( a\\ \\,.., 1o { ())Q.D.J, 5o -.-.trAJn. tr'\\ mo"' rue I ,...i~n.. 10 l i.. -i.-t °°' 1'1, O'\\.- ~ 3 .., -t na. '"* oS D A--t k,.J At.. I .;?5 "\\\\l n0..11...b'\\ I '2. I "l.'4 "'4 '"". o\\ J 3 J-l.~~G\\ 10. I l.. -rJA U)flN JD\\ l 5-o "l.4nc-.,-,.r\\ I

2.

1.--1 O'\\ 1'1.oS J J "l."i U'°I ID I I "1.../A ~ JO I I.sf \\ "l. '4 U"\\ \\.,_. () f l 2-Laboratory control standard (LCS): J ~ Reference standard True value {TV) ~ mple Begin End number (mg CaCO,/l) ume ml ml (ml) INSS.:2:l~S 40 J so o(\\c,.O ..:78:o I Reviewed by: Page~ Page '+ of---1_ Hardness (SM 2340 C-2021) Matrix: Water, RL = 5.0 mg Ca(Q3/L Normality (N) of EOTA pH Factor or Multiplier = 0.2/E = (N,c 50000)/ SO ml sample range = 0.0180

• 0.0220)

N,c 1000 Pi:::.L, Total Multiplier Hardness {MV)

Y. RS = MV / TV,c 100 ml (me c.acO,/l) (acceptable ranee = 90 to 110' J.o ~'0.2 J4o 1cc.o'°l,:o Hardness %RPO Total Multiplier (mg CaCO,/l) = {(5

• 0) /[(5+0)/21} ll 100 ml (acceptable range= t 10%)

30 ~~ s LI I 3.o D e,5L, - 0'i.14.N ) ~) Begin End Total Multiplier Hardness ml ml ml (me CaCO,/l ) 65'... D'l,' -4,') J 32.lP 35.5 ~.,

Jo~

sci b'5.$'.36.to a.1 I ~~ l i.v,=\\c=, _q I.~ I ~ \\, 3q.C\\ '-+I 'O l.l c,(~ 41,o '-l~A I J... ,Jv, 0,0 ,J,:, ~.o 4'0 ~-0 3.1& ). g I' i) I 10 _l& 3,g s: 'l-1.'i ~'6 5.i' '~-~ ~ -ll ,~ ,~.~,.,.~ l-.o ~ I'\\.~ ~v.'5 1..l 14c ~~-'5 3\\.?. 4.1 /. ) \\qo 31.dl 33.v J.4 ( ) q-=r 3~.i.- ~1- ~., l 2> ~&;

• 1-4<>,4

'4{,,l '5'. g l..:>o o.~ 5'." SA IJo 5.~ l~..o l., \\ I.Jr. l~.o ll * ?, '4.; 'i'"l \\\\,.; -21.; 5.o 100 o? 1.; clli..o

~

-,5 Total Multiplier Hardness % RS =MV /TV,c 100 ml (me CaCO,/l) {acceptable range = 90 to 110% <<-O ~o.~ ---+o I oo. n ()jQ v\\._ Date reviewed: loci.1-s.2..u Page 65 of 93

Sequoyah Nuclear Plant Biomonitoring September 10 - 17, 2024 Appendix D Reference Toxicant Test and Control Chart Entered by: J. Perez Reviewed by: J. Sumner Page 66 of 93

Entered by: J. Perez Reviewed by: J. Sumner u ~ ...J ~ -U'I N u n:I ""C I " Pimephales promelas En vironmental T~stlng Solutions. Inc. Chronic Reference Toxicant Control Chart Source: In-house Culture 0.9 0.8 0.7 0.6 0.5 1.0 0.8 0.6 0.4 Control Limits(+/- 2 Standard Deviations) ..._. ___________. ----~-----....... - ~ --------..._...--.* Warning Limits

• • • • t**~***********************

- *~-.. -* ...............................................................,.. t............, **,.................................................... Test date 7-day IC25== 25% inhibition concentration. An estimation of the potassium chloride concentration which would cause a 25% reduction in Pimepha/es growth (calculated using ToxCalc). Central Tendency (mean logarithmic IC25 converted to anti-logarithmic values) Control Limits (mean logarithmic 1~5 +/- 2 standard deviations converted to anti-logarithmic values) . *-. Laboratory Warning Limits (mean logarithmic IC25 +/- 2 coefficent of variations converted to anti-logarithmic values)

• * ** * * *** USEPA Warning Limits (mean logarithmic IC25 +/- SA_75 converted to anti-logarithmic values, SA.75 = 75 th percentile of CVs reported nationally by USEPA)

Page 67 of 93

Entered by: J. Perez Reviewed by: J. Sumner £r1v1,oon,11111.1J TeM.i.1'19 Sohnlon,4, 11\\c Pimephales promelas Chronic Reference Toxicant Control Chart Source: In-house Culture Log10 Conversion Anti-logarithmic Values (g/L KCI) Test number Test date 7-day IC25 Laboratory Calculated CV Toxcal Determination 7-day IC25 CT s CT Control Limits Warning Limits (g/L KCI) CT-2S CT+ 25 CT-2CV CT+ 2CV 1 03-07-23 0.7096 -0.1490 -0.1706 0.0234 0.6752 0.6063 0.7520 0.5732 0.7889 2 04-04-Z3 0.7592 -0.1196 -0.1680 0.0260 0.6792 0.6025 0.7656 0.5663 0.8064 3 05-02-23 0.7295 -0.1370 -0.1656 0.0266 0,6829 0.6042 0.7718 0.5677 0.8131 4 06-06-23 0.6800 -0.1675 -0.1657 0.0266 0.6828 0.6041 0.7717 0.5676 0.8130 5 07-11-23 0.7387 -0.1315 -0.1632 0.0273 0.6868 0.6056 0.7789 0.5686 0.8208 6 08-08-23 0.6677 -0.1754 -0.1637 0.0274 0.6860 0.6045 0.7784 0.5673 0.8207 7 09-12-23 0.6098 -0.2148 -0.1677 0.0287 0.6796 0.5954 0.7757 0.5557 0.8211 8 10-03-23 0,7447 -0.1280 -0.1651 0,0299 0,6837 0.5959 0.7846 0.5552 0.8312 9 11-07-23 0.6718 -0.1728 -0.1631 0.0278 0.6869 0.6045 0.7806 0.5669 0.8232 10 11-07-23 0.6562 -0.1830 -0.1622 0.0268 0.6883 0.6085 0.7786 0.5724 0.8194 11 12-0S-23 0.7102 -0.1486 -0.1617 0.0269 0.6891 0.6088 0.7801 0.5725 0.8211 12 01-09-24 0.6671 -0,1758 -0.1624 0.0271 0.6880 0.6073 0.7795 0.5707 0.8209 13 02-06-24 0.6628 -0.1786 -0.1640 0.0270 0,6854 0.6052 0.7762 0.5684 0,8179 14 03-05-24 0.6019 -0.2205 -0.1666 0.0298 0.6814 0.5940 0.7817 0.5531 0.8286 15 04-09-24 0.685S -0.1640 -0.1660 0.0298 0.6823 0.5949 0.7825 0.5542 0.8292 16 05-07-24 0.7569 ~0.1210 -0.1627 0.0309 0.6876 0.5965 0.7927 0.5551 0.8404 17 06-04-24 0.6610 -0.1798 -0,1656 0.0295 0.6830 0.5963 0.7823 0.5561 0.8283 18 07-09-24 0.6789 -0.1682 -0.1650 0.0293 0,6839 0.5976 0.7826 0.5577 0.8283 19 08-06-24 0.6287 -0.2015 -0.1667 0.0304 0.6813 0.,5922 0.7837 0.5506 0.8316 20 09-10-24 0.7128 -0.1470 -0.1640 0.0295 0.6856 0.5983 0.7855 0.5583 0.8313 Note: 7-day 1c,. = 25% Inhibition* concentration. An estimation of the. potassium chloride concentration that would cause a 25% reduction In Pimephales growth (calculated using ToxCalc). CT= Central tende_ncy of the 1.C,. values. s = St;indard deviation of the IC1s values. Control Limits= Mean logarithmic 1C25 't 2 5tandard deviations converted to anti-logarithmic 11alues. Warning Limits = Mean logarithmic IC1s +/- 2CV or S.,15 converted to anti-logarithmic values. sA.75 = Standard deviation corresponding to the 751 h percentile of CVs reported nationally by USEPA iSA,,, = 0.38). CV" Coefficient of variation. 75th Percentile CV Warning Limits CT-SA.75 CT+ SA,75 0.4186 0.9318 0.4211 0,9373 0.4234 0.9424 0.4233 0.9422 0.4258 0.9478 0.4253 0.9467 0.4214 0.9378 0.4239 0.9436 0.4259 0.9479 0.4268 0.9499 0.4273 0.9510 0.4266 0.9495 0.4250 0.9459 0.4225 0.9404 0.4230 0.9416 0.4263 0.9489 0.4234 0.9425 0.4240 0.9438 0.4224 0.9402 0.4250 0.9461 ~ -Ml.y -;r* Page 68 of 93

Entered by: J. Perez Reviewed by: J. Sumner '\\----- Pimepha/es promelas t 1Wtl1Hl-nt1t1T.<<1n9 $1tlu1IOfH, lll'll, .c: ~ 0... C, 0... r:: 0 u -e C: 0 ~ RI RI > 0.... C: Cl.I *u i Cl.I 0 u 1.25 1.00 Qj n, c= ~ 0.75 n,

5 a.so ao E - 0.25 20

.r. .... 3 0... C, 0 10 C: 0 u... 0.... 0 30 20 10 Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture USEPA Minimum Acceptance Criteria(> 0.25 mg per surviving larvae) USEPA Maximum Coefficient of Variation Guidance Criteria(< 20%) USEPA Lower PMSD Bound (>12%) USE PA Upper PMSD Bound Acceptance Criteria(< 30%) ~~- ---....-...,..,,.~ ~~*-------------

---

~~-----~~-----~---------

Control Growth, Coefficient of Variation (CV) or Percent Minimum Significant Difference (PMSO) PMSD is the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is not a minimum acceptance criteria. Central Tendency (mean Control Growth, CV or PMSD) 95% Confidence Interval (mean Control Growth, CV or PMSD +/- 2 Standard Deviations) n~bV m Sur,,ne, ~ Page 69 of 93

Entered by: J. Perez Reviewed by: J. Sumner r-.,-,w~ Pimephales promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture ToxCal Determination Control Growth Control Growth CV Control Control Growth Test number Test date Survival Mean CV MSD (%) (mg/Initial lant* e) (%) 1 03-07*23 100 0.719 11.0 0.0858 2 04-04-23 100 0.651 4.1 0.0900 3 0S-02-23 100 0.737 6.S 0.089S 4 06-06-23 100 0.734 11.1 0.0988 s 07-11-23 100 0.630 9.5 0.0976 6 08-08-23 100 0.809 11.9 0.1504 7 09-12-23 100 0.707 8.5 0.0796 8 10-03-23 100 0.678 7.7 0.0772 9 11-07-23 100 0.797 4.0 0.0880 10 11-07-23 100 0.833 6.8 0.09.13 11 12-0S-23 100 0.635 8.6 0.0805 12 01-09-24 100 0.776 5.8 0.0923 13 02-06-24 100 0.753 12.8 0.1364 14 03-05-24 100 0.873 10.S 0.1016 15 04-09-24 100 0.653 7.8 0.0834 16 OS-07-24 100 0.693 11.9 0.0896 17 06-04-24 100 0.693 8.0 0.0862 18 07-09-24 100 0.683 9.0 0.0883 19 08-06-24 100 0.796 3.9 0.0766 20 09-10-24 100 0.701 7.0 0.0964 Nottt: Control SUf'/IV~I: USEPA minimum test acceptability cnierlo ~ 80"-' survival. Control Mean Growth: USEPA minimum test acceptability criteria~ 0.25 mg/surviving larvae, OJ: co~ffic1ent of variation for control growth. USEPA maximum CV guidance criterfa (90° 1 percentlle) < 20% MSO

• Minimum slgnfflC'ant difference.

PMSD = Perc*ent minimum s1gnlflcant difference. Test lmg/lnltl*I lan,ae) (%) PMSD CT 9S% Confidence Interval CT 9S% Confidence tnten,al f"I CT *25 CT+ ZS CT*2S CT+ 2S 11.9 0.745 0.609 0.881 8.5 1.4 15.6 13.8 0.742 0.601 0.884 8.3 0.9 15.6 12.1 0.743 0.602 0.884 8.3 1.0 15.6 13.S 0.749 0.619 0.878 8.4 1.0 15.8 15.5 0.742 0.603 0.882 8.6 1.3 15.9 18.6 0.741 0.603 0.879 8,6 l.3 15.9 11.3 0.740 0.602 0.879 8,6 1.3 15.9 11.4 0.739 0-.599 0.880 8.7 1.5 15.9 11.0 0.748 0.616 0.880 8.7 1.7 15.8 11.0 0.750 0.614 0.886 8.9 2.5 15.4 12.7 0.746 0.602 0.890 9.0 2.5 15.4 11.9 0.740 0.614 0.866 8,9 2.4 15.4 18.1 0.736 0.618 0.855 9.2 2,5 15.9 l-1.6 0.742 0.609 0.876 9.1 2.6 15.S 12.8 0,736 0.598 0.874 8,7 2.8 14.6 12.9 0.734 0.595 0.874 9.0 3.0 15.0 12.4 0.732 0.591 0.872 9.1 3.2 14.9 12.9 0,724 0.591 0.857 9.0 3.2 14.7 9.6 0.728 0.591 0.865

• 8.4 3.0 13.7 13.8 0,728 0.591 0.865 8.3 2.9 13.7 PMSD ts a measure of ten pr~dsion. Thi! PMSD h the mlnlmum percent dlff~rence be,twe~n the control *.ind treatment thilt can be declared statittlc.ally sl1nrf1cant tn a Whore. effluent to)(lclt'{ tt s-t, LowerPMSD bound determined by USEPA (10" porcentlle.) > 12%.

The. lower PMSD bound repre.sents: a practk.al 11m~t to the 1en.slt1vlty of (h~ ten method and Is not o minimum accept1nce cr1terta. Upper PMSD bound.cceprance criteria d*tormined by USEPA 190"' percentile) < 30%. CT= Central tendt!ncy of the growth, CV or PMSO Vilue.s. S : Stondard deviation ol the growth, CV or PMSD values, CT 13.0 13.0 12.8 12.8 13.1 13.4 133 13:2 13.1 13.2 13.2 13.1 13.7 13.3 13,1. 13,1 13.1 13.1 13.0 13.2 Test PMSD 1%) 95% Corifidence Interval CT-2S 8.1 8.1 8.0 8.0 8.4 8.0 7,9 7.7 7.6 7.8 7.8 7.7 8.4 8.7 8.7 8.7 8.7 8.7 8.3 8.7 CT+ 2S 17.8 17.9 17.6 17.6 17.8 18.7 18.7 18.6 J8.6 18.6 18,6 18.6 18.9 17,8 17,6 17.6 17,6 17.6 17.7 17.6 r:.-- ... ~f Page 70 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page l of 6 Potassium Chloride Chronic Reference Toxicant Test {EPA-821-R-02-013, Method 1000.0) Species: Pimephales prome/as PpKCICR Test Number: 119 Dilution preparation information: Comments: KCI Stock INSS number: INSS '"l.~V\\ Stock preparation: 50 g KCl/l: Dissolve SO g KCI in 1-L deionized water. Dilution prep (mg/L) 300 450 600 750 900 1050 Stock volume (ml) 12 18 24 30 36 42 Diluent volume (ml) 1988 1982 1976 1970 1964 1958 Total volume (ml) 2000 2000 2000 2000 2000 2000 Test organism information: Test information: Organism source: In-house culture Randomizing t emplate: '( Ill nvJ Age: < 24-hours old Incubator number and shelf ,f) location: Spawn date: 01\\*0'1*"-.\\ Artemia CHM number: CHM1294 Hatch dates and times: O~* CJ'\\*\\.-\\ \\'\\S~ " ~ -111-~ 1-\\0S Drying information f or weight determination: Transfer vessel informatfon: pH:; 6-'~ s.u. Date/ Time in oven: I il'\\*I'\\* \\.i nl.AC.. Temperature= °L-\\,0 oc

• Initial oven temperature:

uo* L Average transfer volume: < 0.25 ml Date/ Time out of oven: ,Ol\\-lf *i.--\\ flll'k

• Final oven temperature:

l.o*'- Total drying time: "'\\.-\\*~\\)Jl~

• 60°C Oven, Thermometer SN: 14-98565 Daily feeding and renewal information:

Day Date M orning feeding Afternoon feeding Test initiation, renewal, M HSW or termination batch used Time Analyst Time Analyst Time Analyst 0 09-10-24

osu, IA

,~~(> o, \\\\ i cf\\-()\\l,.* l.-\\ ~ 1 09-11-24 I c<;co IA \\\\00 \\ { 0,0C) u L 2 09-12-24 I.A r\\.... Ot:\\* o'\\* "L~ ~ r,<.,..,-. I-, n l'\\ cnoe:> .h 3 09-1.3-24 \\A u. (l"'\\ t) t) " l'f ~ l:)r, na \\\\~() V 4 09-14-24 \\4 V U' V C>'\\- QC!t.* \\."\\ 1)1. N\\ \\"'I r,r-.. ol 011 l"I t> s 09-15-24 0\\:.0 l) ~ -CO ~ o,oo \\ 6 09-16-24 0$0[). \\It \\\\t>O vt CJ'\\()0 v\\ .1, 7 09-17-24 I

• u -

O':::,\\ X. \\\\. Chemical analyses: Parameter Reporting Limit M ethod number M eter Serial number pH O.lS.U. SM 4500-H+ B-2021 Accumet AR20 93312452 Dissolved Oxygen {D.O.) 1.0 mg/L SM 4500-0 G-2021 YSI Model S2CE 08A100271 Conductivity 14.9 µmhos/cm SM 2510 B-202.1 Accumet AR20 93312452 Alkalinity 5.0 mg C3C01/L SM 2320 B-2021 Accumet AR20 93312452 Hardness 5.0 mg CaC01/L SM 2340 C*2021 Not applicable Not applicable Temperature o.1*c SM 25S08-2010 Digital Thermometer 1\\Q~,'\\~&,<; Control information: Acceptance criteria Summary of test endpoints: o/o Mortality: en. $20% 7-day LCso ( rng/l KCI) 'l'\\1.t\\ Average Weight per initial larvae: l").10\\ NOEC (mg/l KCI) 1,1\\f\\ Average weight per surviving larvae: 0-10\\ ~ 0.25 mg/larvae LOEC (mg/L KCI) ~I\\ ChV (rng/L KCI) 1,10. a ICzs (mg/l KCI) ,,1..1 SOP AT21-Revision 5-Exhibit AT21.l Page 71 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 2 of6 Species: Pimepha/es promelas PpKCICR Test Number: 119 Survival and Growth Data Day Control 300 mg KCI/L 450 mg KCI/L A B C D E F G H I J 0 10 10 10 10 10 10 10 10 10 10 1 I l) I () ' () It> I l) 10 10 I 0 10 10 2 I l) I {) IQ I D

11)

IQ I 0 I(} I{) I () 3 '0 lO to I 0 l 0 l D 10 /() I 0 lb 4 10 10 10 I \\) l I..) l 0 {0 10 ,o I 0 5 ID ,~ It> ID 10 ID 10 10 ID /0 6 I 0,~ , C) '0 (0 / 0 I 0 10,~ I~ 7 10 I \\'i IU 10 /iJ IQ 'D ( (). I 0 'O

• A = Pan weight (mg)

Tray color code: mr,of-l e,Jt>f..,,..... V(fo {bib Analyst: f'.A/7 '?A. o' 'O" 10 s"" °'I

• J(

~:~ .,),'.\\, <o Date: a.f/.-ztz-z-+ ~- v<.. \\: ~- vt' v<_* 0 * ~ - I'

• a =-Pan+ Larvae weight {mg) 11..,-t Analyst:

I { 1\\,4,\\, l,\\* ~ \\ II\\. 1.\\ 1.1.01 lM,i '20,l C..,.,. a, "1.\\-1.'l 1.1, <i1 Date: V ~

• I'\\-~

C = Larvae weight {mg) = B - A !..st\\,.~ rv:.'-' '-* <:al\\...,o. &.O\\t i, lo,.,.~t .... !IA l.,'\\-t Analyst: ~ u Weight per initial number of larvae (mg) = C / Initial number of larvae ~ ~ ~..:;,"' ~ ~~ N t} ~ ~ i \\I ~ ~,.. \\!) Analyst: o* ~* o* o* ()° o* i;)' o* o* Average weight per initial Percent number of larvae (mg) reduction 0.10\\ o.-i'"\\I., - 10.,"l. o.1ol:\\~ from control (%) "'Weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520. Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg= unusually large, d&r = decanted and returned, w = wounded. Comments: K L 10 10 ID ID I Cl IV 10 It) I 0 JO I t, tO 10 IV 10 10 ~ .;/.;J.._ ~- 0* 11.1.0 1a.i'~ c.,'l'-(,.*fo ~ ~ \\D A o* ()* 0.1.7. SOP AT21-Revision 5-Exhibit AT21.1 Page 72 of 93

Entered by: J. Perez Reviewed by: J. Sumner Page 3 of 6 Eswironmt:nt*I Tei Ung SoludonL IIK-Species: Pimephales promelas PpKCICR Test Number: 119 Survival and Growth Dato Day 600 mg KCI/L 750 mg KCI/L 900 mg KCI/L M N 0 p Q R s T u V 0 10 10 10 10 10 10 10 10 10 10 1 '\\) I 0 / 0 /() '\\ '<( 't I<( i,<(. 1*~ s s.r.( ss" 2 10 10 I \\l IU I\\ °' ~ 4 '" c; 3 I \\) 10 I l) t\\ '\\ ,ol °' ~ ~ I 0 1 4 10 I t> IC 10 '\\ s'..<.., l\\ '-\\ s 5 ct"- 1/2: ~,.(' 10 ~ It) IU it 7 °' 1.. 6 ., 2.~ 10 C\\ IQ I 0 E t 7 I--' !) 7 10 G\\ LO I\\) 8 i 'l,o\\.

• A= Pan weight (7=!)

Tray color code: Jff7.r Ml Pe/y 01>,, "}'a o"'> Analyst: ff' -i,"v ~ \\. c,:'\\ /\\ \\ ~\\'.) <--,~ c._O Date: Cl~.-1.b~'Vt- ~ - -~

• o* :y-0 **

(\\,. ~* ~ l'\\1 '

• B = Pan+ Larvae weight {mf)

Analyst: J "26,i&,.a.ss 1(,. r,t' ti.Sb 1t.&'t 11.(..S 2,(:).t\\ 1&,4'?a *S-o\\ *~-lo\\ Date: t!'\\*IA*l.~ C = Larvae weight {mg) = 8 -A ,.':>>b t..1-llD i..,s -,A'\\ "'* i':i ~.C\\'\\ ~.oa s...., ,.-41 1,-, l Analyst: ~ Weight per initial number of larvae (mg) = C / Initial number of larvae 114.t.,

I

~ ~

).

),..

0\\ ~ ~ ~ ~ ~ I.; ~ Analyst: A'.\\ t:;J' o* ()' -o* 0 c o* 0 o* o* Average weight per initial Percent number of larvae (mg) reduction o.i.o'\\o from control ,.~ 7.

0. ':,cf\\

1.1.'3/4 7. 0.1 s'-\\ (%)

• weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520.

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg= unusually large, d&r = decanted and returned, w = wounded. Comments: w X 10 10 s~ s 5.(, ) s s -.l '~ '-\\ '" 1-l "::,~ !>,...\\ .ii\\ L- '2. L. \\ *~ '>,11 \\)'1 ,"J* ,s.1~,..,, &,C, 11...0b o,4! ~"' ~ ~ a* o* .,,.01. SOP A T21-Revision 5-Exhibit AT21. l Page 73 of 93

Entered by: J. Perez Reviewed by: J. Sumner (nYlrontMnl al luting SotuUoru. Inc. Species: Pimephales promelas PpKCICR Test Number: 119 Survival and Growth Data Day 1050 mg KCI/L V z AA BB 0 10 10 10 10 1 -f II~ 41 t.d. ~4,c( ~ i..,( 2 '-\\ '-\\ ~ '-\\ 3 ~ ~ y y 4 15"',_v'-

• ~

~ ~ 5 V. \\ ii "\\.~ \\~ I I \\ 6 l 0 () \\ 7 0

• .i_

0 0 0.~

• A= Pan weight (m~)

Tray color code: nreS} (bre('/\\., \\o/\\ ~ Analyst: ~ ,.I\\. 0 Date: al;,-2:'1-2,':f ..x* ~- <:V '( '

• e =Pan+ Larvae weight (mg)

Analyst: Date: l.---.-- C = Larvae weight (mg) = B - A

---

=========

Analyst: 0"-**1'1: Weight per initial number of larvae (mg) =CI Initial number of larvae 0 0 0 0 Analyst: i Average weight per Initial Percent number of larvae (mg) reduction 0 ,oo 1, from control (%)

• Weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520.

Comment codes: c= clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg= unusually large, d&r = decanted and returned, w = wounded. Comments: Page 4 of 6 SOP AT21-Revision 5-Exhibit AT21.1 Page 74 of 93

Entered by: J. Perez Reviewed by: J. Sumner ~Yironrn~11to1f TdUnv SolUOO(IS, Inc.

Cont~h-.Uo" (m&/l l

tldty te,st. Lowtr PMSO bound deh!rmlnHt by USEPA (loth petc,~ntit11) ; 12%. Upper PMSO bound de1ermlned by US EPA (!10th percen~I*) = 30,,:. Lowar li!"ld upper PMSD bounds were determined from thtt 10th.and 90th perc:cntile1 respectivt!IV, of PMSO diltoll from E'PA'1 Wf:T' 1nterlaboratory V'u1abltlt-,i Studv (US EPA, 2001.a; USEP~ 1001b). The lower PMSD bound rll!!presentl 41 practk *1 lfmit to.the ff'nsitivit,; of the t~st method and h not a minimum.tcce.ptanc:o Griterl*. USE.PA. 200h, 2oou,. Final Report: lntertaboratorv Vt1rl1bility Study of £PA Short**tll!!rm Chronk: *and Acute Wf\\ota £ffluerit Toxkrr; T~t Methods, Volumu l ind PAppendf11:. £PA..S21.-B*C>1*004-and *EPA..S21-8.01,,00S_ U5 Environmental Protec.uon Ar*ncy. C1ndnn1tl, OH, ...,, :.~. Page 75 of 93 Entered by: J. Perez Reviewed by: J. Sumner Statistical Analyses Larval Ran QrO,Ntn and SUNtvalTHl-7 Day Surwnl s1ar1oa1e* 9/10/2024 Tesl lD: PpKCICR Sample ID: REF-RelToxlcant End Date. 9117/2024 Lab ID' ETS-En'1r, Testing Sol Sam pie Type: KCL-Potass1um clllor,de Sam pla Dale: Proiocol: FWCHR*EPA-821 *R-02*013 Test Specie.. PP-P,mephales promelas Commenls: conc,ma11. 1 2 J 4 D-Control 1.0000 1.0000 1.0000 1,0000 300 1,0000 1.0000 1.0000 1.0000 450 1,0000 1 0000 1,0000 1,0000 600 1.0000 0.9000 1.0000 1,0000 750 0.8000 0.7000 0.7000 0,7000 900 0.2000 0.2000 0,2000 0,1000 1050 0.0000 0.0000 0.0000 0.0000 Transform: Arcsln S!!!!!** R-Rank t -TaU.d Number Total COIIC;-fl!a/l M**" N'M*an Mun Min Max CV% N Sum Critical Reap Numl>ar D*Contro\\ 1.0000 1.0000 1.4120 1,4120 1.4120 0,000 4 0 40 300 1.0000 1.0000 1 4120 1,4120 1.4120 0.000 4 18,00 10.00 0 40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0,000 4 18.00 10.00 0 40 600 0.97QO 0.9750 1.3713 1,2490 1,4120 5,942 4 16.00 10,00 1 40
• 750 0.7250 07260 1.0202 0.9912 11071 5.685 4
10,00 10.00 11 40 '-900 0.1750 01750 0.4282 0,321 a 0.46l6 16,570 4 \\0.00 10.00 33 40 1050 0.0000 00000 0,1588 0,1588 0,1588 0,000 4 40 40 AuXlltarv Testa Statistic Critical Skew Kurt I Shaplro-lMlk's Test Indicates non-nonnal dlstflbuUon IP= 0.01) 0,83255 0.884
• 1.1518 2.78665 Equalitv or valiance cannot be confirmed
-11es1s Tut n.cau, o.os1 NOec LOEC ChV ru I Steel's Many-One. Rani< Test 600 750 67082 Treatments vs D-Control Maximum U~llhc>ocl-Probll Parameter Vallie SE 15% Rduclal Umb Control Ct.Sq CritlGal p.ya.... Mu Sigma llar Slope 18.5884 2.49982 13.6888 23.488 0 1.5524 9.48773 0.81732 2.90193 0.0538 4 lntorcept -48.942. 7.2.747'2 ~3.201 -34,684 TSCR 1,0 f'olnt Problta mall. 95% Rducial Llrnlla EC01 2.674 598,113 532,204 64_2,194 0.9 EC05 3.355 850,797 595.378 688.26 0.8 EC10 3 718 680.754 631,593 714.684 0 7 EC15 3,964 701.741 656.938 733.449 EC20 4 158 718.881 677,525 749,022

0) 0,6 EC25 4.326 733.919 695.43 762,941 i 0.5 EC40 4.747 773223 740.962 801,022 EC50 5000 797,873 768.07 826,672

~ 0.4 EC60 5.253 823 31 794,513 854,923 0.3 EC75 5.674 867.4 836.711 908.13 l EC80 5.842 885,545 852.98 931,343 0,2 EC85 6,036 907.175 871,758 959,796 0.1 EC90 6.282 935.142 895.276 997.608 EC95 6.645 978.187 930,285 1057,57 0.0 1 10 100 1000 10000 EC99 7.326 1064.35 997,687 1182 3 Dose mg/L Oose-ReIp0nse Plot 0.9 0.8 07 i o.e ~ o.s >, a OA 0.3 0.2 0.1 0 g ~ ~ ~ 5l ~ ~ l5 r.- g Page 76 of 93 Entered by: J. Perez Reviewed by: J. Sumner Statistical Analyses Lamal Atth Growth and Survl""I THt-7 Day Growth jStan Date: 9/10/2024 Test ID: PpKCICR Sample ID* REF-RerToxlcant End Dale; 9/17/2024 lab ID: ETS-Envlr. Testing $01. Sam pie Type* KCl-Potasslum chloride Sam pie Date: Protocol: FWCHR-EPA-821 *R-02-013 Test Speci es. PP-Pimephales prometas Comments: Conc-mgll. 1 2 3 D-Control 0.6590 0.7300 0.7540 0.6590 300 0.6790 0.8040 0.8620 0.7580 450 0.6690 0.6940 0.6940 0.7400 600 0.7560 0,6460 0.6150 0.7440 750 0,4850 OA940 0.5080 0.5470 900 0.1470 0.1710 0.2060 0.0930 1050 0.0000 00000 0.0000 0,0000 Traneform: Untransrormad 1,TNad lsoConlo Conc-111gll. Mean N-Mean Mean Min Max CV% N ..Stat Crltlcal MSO IIHn N-Maan I D-Control 0.7005 1.0000 0.7005 0.6590 0.7540 6.982 4 0.7381 1.0000 300 0.7758 1.1074 0.7758 0,6790 0,8620 9,961 4 -1.787 2,290 0.0964 0.7381 1.0000 450 0.6993 0.9982 0.6993 0.6690 0.7400 4 235 4 0.030 2.290 0.0964 0.6993 0.9473 600 0.6903 0,9854 0.6903 0.6150 0.7560 10.187 4 0.243 2.290 0.0964 0.6903 0.9351 7.50 0.5085 0.7259 0.5085 OA850 0.5470 5.380 4 0.5085 0.6889 900 0.1543 0.2202 0.1543 0.0930 0.2060 30.781 4 0.1543 0.2090 1050 0.0000 0.0000 0.0000 0.0000 0.0000 0.000 4 0.0000 0.0000 , AIIXlllrv Teats StaQstlc CritJcat Skew Kurt
• Shaplro-Wilk's Test,ndleates normal dlstribullon (p > 0.01) 0.96531 0.844
-0.1234 -0.9219 I Barllett's Test indicates equal variances (p = 0.48) 2.46911 11.3449 I HypolhHls TH I (1 slall, 0.05) NOEC LOEC ChV T U MSOU MSDD MSB MSE F-Proo ,;if Ciunned's Test 600 >600 0.09643 0.13765 0.00634 0.00355 0,2031

3. 12 Treatments vs -Control un.. r 1nterpo1allofl,aoo Re**mP'HI Pofflt mg/L SD H%Cl.(Exp)

Skew IC05 442.40 94,81 335.36 734,02 0.4719 IC10 621.41 52.67 360.82 669.84 -1.7608 IC15 651.87 24.21 569.22 695.40 -1.4655 1.0 IC20 682,32 19.02 609,57 721.65 -0.8225 0.9 IC25 712.78 16.45 650.55 749.57 -0.4818 IC40 777.79 6.25 756.63 795.37 -0.0611 0.8 IC50 809.04 5,75 789.39 825.47 -0,0310 0.7 08 .. 0.5 l 0.4 I 0.3 0.2 / 0.1 / 00.......... -- ~;------' -0,1 -0.2 0 500 1000 1500 Dose mg/L Dose-RHponso Plot 1 0.9 0.8 ~T 0.7 T 1 o.5 -------- -------------------------- ------ 1-tail, 0.05 leval of s1gnificance 0 0.5 >, a o.4 0.3 0.2 0.1 0 I ~ ~ ~ ~ § ~ Page 77 of 93 Entered by: J. Perez Reviewed by: J. Sumner Page 5 of 6 En.vlronmcnt.1ITe11ln9 So\\udoM. In(, Species: Pimephales promelas PpKCICR Test Number: 119 Daily Chemistry: Temperatures performed at the time of test initiation, renewal or termination by the analyst identified In the Daily Renewal Information table located on Page 1. Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet. Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 0 1 2 Analyst \\.A- ~ t'\\, /l.M,I\\J ~ &A- /f,v1 ~ \\.A,t Concentration Parameter pH (S.U.) -q::}5 7,.e-0 7. 1.{-9

7. I 2, 7, 3"2-4.l<o Dissolved oxygen

~.,. I}~ ~~ 'l~ 1.\\,(1 <3f\\ (mg/L)

CONTROL, Conductivity 2'1~

~18 MHSW (µmhos/cm) 130 Alkalinity (mg CaCOi/l) l.-.l ...aal \\...\\ Hardness (mg CaCO,/L) .e1 R'\\ Temperature (0C) '"l..--\\." "\\S*,_ "\\"-. l 7.4. \\ ~'-l. ~ "\\.-\\.. f pH (S.U.) ~e:P 7 29.

7. ~~

7 7,.,t, --; '-I:~ 1-.,~ Dissolved oxygen 4~ '10 A,.a, 1A "t.l 5~ 300 mg KCI/L (mg/L) Conductivity M9J a .:>11ui-1 (µmhos/cm) 8 ',O ~ I.Jl,l 889 f"5?f Temperature {0C) -u.o "-'*0 "\\.~-cf '\\.-l,<\\ ~ -'\\ "\\.c;.. \\ pH (S.U.) /.},':},~ 7, 3'1 7.ti 3 7 -z,c; 7, '-f P. ~ -21 Dissolved oxygen l'Jr.Q; 1)-~ 1R ~-'< /j,f '1.'-l (mg/L) 450 mg KCI/L Conductivity (µmhos/cm) 101*0 t L5'o l!lfo Temperature (*C) v,., -u. "L... "\\.'\\,<\\ '\\i. \\ "\\.-\\.'\\ i..S*1-pH (S.U.) '.\\.~ 7./.1-o 7.'{ f< 7, 3'2.. 7,Sl I)., 1-'!, Dissolved oxygen t,}{1 'le> ~A '}.6 r'.}8 b,'2.. (mg/L) 600 mg KCI/L ConductMty (µmhos/cm) l3~0 l 4 3o ltf/o Temperature (0C} '\\.~. \\ ~."L '\\.~. '1 '\\S, \\ "\\S,O '\\S. t) pH (S.U.) '-tH1 7,'-f-3 7,<;;2..

7. 31-7, 5"3

"}.,o Dissolved oxygen 'if1 /"}.~ ~.9 'ts ,tg ~-1 (mg/L) 750 mg KCI/ L Conductivity (µmhos/cm) Iv\\ o L 6qo L blo Temperature (*C) ~*Vi ~'\\-'\\ 'U\\. t "\\.S;. l.. 1.S-o ""l<.. 0 pH (S.U.) "+.} 3 7, tt5 7,bL(- 7, {!?_ 7,56 '12Y. Dissolved oxygen 8.o l},q ~q /},t, q~ 0,l (mg/L) 900 mg KCI/L Conductivity (µmhos/cm) 18q() I 9.?o 1930 Temperature (*C) l.'-\\. c; v,\\." "\\.~- f ~ - 0 1.4, '\\ "\\S*,_ pH (S.U.) (\\._).q 7J .. /-e2. 7,57 7, 4-2... 7.(~ /,'.\\.z.;- Dissolved oxygen B.u t9 0ti /},o l)q ~.. ~ (mg/l) 1050 mg KCI/ L Conductivity (µmhos/cm) ar2,o 1./"lcJ <"]_ISo Temperature {°C) '\\.'4." "'\\. "\\, '\\ 1.'i. y ,s. 1.. 1.~.'l U - 6 Initial Final Initial Final Initial Final SOP AT21-Revision 5-Exhibit AT21.l Page 78 of 93 Entered by: J. Perez Reviewed by: J. Sumner Page 6 of 6 Species: Pimephales promelas PpKCICR Test Number: 119 Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 3 4 s 6 Analyst vL

o.....,

a..~ (,St. 8~l ~ I~ ~ Concent ration Param eter pH (S.U.) 1'\\.Aii'l-

1.10

+.43 1.0-?, i-.l, ' 7.!7
• 7, 3 I 7 /0 (/,
Dissolved oxygen t.+ 1, 1- -:/. 'l- 'l3 1-~ ,.~ 7. \\. 7,=, (mg/L) Conductivity jl)'-\\ ~1 S{O

CONTROL,

(µmhos/cm) ~ 1.... I MHSW Alkalinity \\o'\\ I (mg CaC03/L) Hardness ~-*
• ~
(mg CaC03/L) .g-'\\ ~ Temperature (*c) "\\.~- l- ,~.o "-"* t "\\.Ci*?, "\\."\\,,i "'\\.-l. 4\\ ,.'\\. I '\\..Ci. \\ pH (S.U.) '}J,A l,;l
1. \\.j "\\.'-\\.'\\ pH (S.U.) t,V5" 1.3~ 'l.55

   ,..* n*

   J-""'+

   7.59 7, 75 I 7.36 Dissolved oxygen 'f,1 + -I., 1-.°I 'l,3 'g,.:) 7 - ~ 7,7 ,.'\\_ (mg/L) 600 mg KCI/L Conductivity txo i (µmhos/cm) 13'+0 1?2.0 {Jtt-o Temperature (0C) "\\."\\. t '\\.~. I )..'(. f- '\\r.. o ""\\.S,l) '\\~-~ 1.-t-t \\.-t.'\\ pH (S.U.)
   • t.i.,9

   J.35 1-'6, i,34

   , ~'5 7.5;;

   7~'! ?.tf-Z-Dissolved oxygen 1.i

   J, v

   -=l-~'i ~ -~ ~o -,_ s,. ~ 7...,_ (mg/L) 750 mg KCI/L Conductivity l (µmhos/cm) \\9-lo l5a+C> 1'5q D {S9o Temperature (0C) \\."\\. f '\\.S
   • I
   "\\..~., "'U,0 "\\.~ -0 ~-,_ 1-'-l*l '\\'lo. 'L pH (S.U.) 't.)2- ':l,Ao -l,O

   J.;~

   ,,lt C, 7,61 7-9?/4 7.t-fb Dissolved oxygen

   ~-~ ls i.o 1-.;,. 1-.? .,. \\. ,.~ 7.\\ (mg/L) 900 mg KCl/l Conductivity I '¥1 o I (µmhos/cm) IS3o 1~,~ fSJo Temperature (*C) 1.1.{,1 '\\.~- \\ "'\\5 *O "\\S-' ""\\S,O "l.S-'"L 1.'\\-7 '\\.~.o pH (S.U.) ~.:\\i.l 1.4\\, 1.Lr5 '+r+;).

   J.10 7.6~

   7, 89. 7. if-t.f Dissolved oxygen '16

   J.4 i.o 1--:l

   'l).D \\
   • s 7.1
   ..,_ \\ (mg/L) 1050 mg KCl/l Conductivity I (µmhos/cm)

   i.oi.o lc? t>SD Jo-:}{)

   2.lno Temperature (*C) ~ -'i 1.\\\\. f \\~-&' "l.(.. \\ '1_). 0 1.\\. '"L 1.'-1, 1 ,s. 2.,.. Initial Final Initial Final Initial Final Initial Final SOP AT21-Revision 5-Exhibit AT21.1 Page 79 of 93 Entered by: J. Perez Reviewed by: J. Sumner Ceriodaphnia dubia Envlronme:ntal Te51lng Solutions, Inc. Chronic Reference Toxicant Control Chart Source: In-house Culture 1.12 1.10 1.08 u n, z ~ 1.06 0.0 - Ill N u n, "'C I 1.25 1.20 Control Limits (+/- 2 Standard Deviations} Warning Limits
   • • • • • • • • ~**r********
   • 1.15 1.10
   -**-**-,,,*-**-**---.. *..It.,_,._,,_..,.._~,-**--*-, -... _,,_.. _.* _.,,..-.... 1.05 1.00 Test date 7-day IC25 = 25% inhibition concentration. An estimation of the sodium chloride concentration which would cause a 25% reduction in Ceriodaphnia reproduction {calculated using ToxCalc). Central Tendency (mean logarithmic IC25 converted to anti-logarithmic values) Control Limits (mean logarithmic 1Cz5 +/- 2 standard deviations converted to anti-logarithmic values) Laboratory Warning Limits (mean logarithmic IC25 +/- 2 coefficent of variations converted to anti-logarithmic values) USEPA Warning Limits (mean logarithmic IC25 +/- sA.io converted to anti-logarithmic values, Suo = 10th percentile of CVs reported nationally by USEPA) Page 80 of 93 Entered by: J. Perez Reviewed by: J. Sumner Ert1i1hortmotnlo111I re)-IIPQ SohJlli>n11 lnC", Test number Test date 7-day 11:zs ToxCal Determination 7-day IC25 (g/L NaCl) 1 04-04-23 1.1090 0.0449 2 05-02-23 1.0948 0.0393 3 06-06-23 1.0931 0.0387 4 06-06-23 1.0967 0.0401 5 07-11-23 1.1009 0.0417 6 08-08-23 1.0952 0.0395 7 09-12.-23 1,0815 0.0340 8 10-03-23 1.0922 0.0383 9 11-07-23 1.1010 0.0418 10 12-05-23 1.1000 0.0414 11 01-09-24 1.1048 0.0433 12 02-06-24 1.0884 0.0368 13 03-05-24 1.1004 0.0416 14 03-05-24 .1.0839 0.0350 15 04-09-24 1.0748 0.0313 16 05-07-24 1.1127 0.0464 17 06-04--24 1.0920 0.0382 18 07-09-24 1.0956 0.0396 19 08-06-24 1.0972 0.0403 20 09-10-24 1.1057 0.0436 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Source: In-house Culture Log10 Conversion Anti-logarithmic Values (g/l NaCl} Laboratory Calculated CV CT s CT Control limits Warning limits CT* 2S CT+2S CT-2CV CT+ 2CV 0.0386 0.0038 1.0930 1.0739 1.1125 1.0756 1.1105 0.0386 0.0038 1.0929 1.0739 1.1123 1.0755 1.1104 0.0386 0.0038 1.0930 1.0740 1.1124 1.0756 1.1105 0.0385 0,0037 1.0928 1.0741 1.1118 1.0757 1.1099 0.0388 0,0038 1.0935 1.0747 1.1126 1.0763 1.1107 0.0392 0.0035 1.0943 1.0769 1.1121 1,0784 1.1103 0.0388 0.0037 1.0935 1.0753 1.1121 1,0768 1.1103 0.0386 0.0035 1.0929 1.0754 1.1108 1.0769 1.1090 0.0387 0.0036 1.0933 1.0753 1.1115 1.0769 1.1097 0.0389 0,0036 1.0937 1.0756 1.1122 1.0772 1.1103 0.0389 0.0037 l.0938 1.0755 1.1125 1.0771 1.1106 0.0390 0.0036 1.0940 1.0760 1.1123 1.0775 1.1105 0.0392 0.0036 1.0944 1.0761 1.1129 1.0777 1.1110 0.0387 0.0036 1.0932 1.0754 1.1113 1.0769 1.1095 0.0383 0.0039 1.0922 1.0726 1.1121 1.0743 1.1101 0.0388 0.0043 1.0934 1.0720 1.1152 1,0738 1.1130 0.0390 0.0041 1.0940 1.0734 1.1150 1,0752 1.1128 0.0394 0.0038 1.0949 1.0760 1.1142 1.0777 1.1122 0.0393 0.0037 1.0947 1.0760 1.1138 1.0776 1.1119 0.0398 0.0037 1.0960 1.0777 1.1146 1.0793 1.1126 10th Percentile CV Warning limits CT-s.... 10 CT+ SA.10 1.0056 1.1805 1.0055 1.1804 1.0056 1.1805 1.0054 1.1802 1.0060 1.1810 1.0068 1.1819 1.0061 1.1810 1.0055 1.1804 1.0058 1.1807 1.0062 1.1812 1.0063 1.1813 1.0065 1.1815 1.0068 1.1819 1.0058 1.1807 1.0048 1.1796 1.0059 1.1809 1.0065 1.1815 1.0073 1.1825 1.0072 1.1823 1.0083 1.1836 Note: 7-day IC,s ~ 25% lntiibitloo *concentration. An estimation of the sodium chloride concentration that would cause a 25% reduction in Ceriodophnio reproduction (calculated using ToxCalc). CT =- Ce"ntral tendency of the IC,. values. S = Standard deviation of the 1Ci5 values. Control limits= Mean logarlthmic IC,.+/- 2 standard deviations converted to anti*logarithmic values. Warning limits= Mean logar1thmlc IC25 +/- 2CV or SA.,o converted to anti-logarithmic values. s *.,0 = Standard deviation corresponding to the 10'h percentlle of CVs reported nationally by USEPA (SA.,o =- 0.08). CV= Coefficient of 1/ariatlon. "'"~... "-"""...._o1111v ,~j"" Page 81 of 93 Entered by: J. Perez Reviewed by: J. Sumner C~IMI Jof\\11'10 Sokn~.. ** 40 C - 35 0 cu
   • ~ ni u

   , E 30

   "'O Cll o~ g-tlO 25 a:::.E 0... Q. 20 II) ... :i: C 0 o_ 15 u 10 ~ C 40 0 C 0 V ',p

   , 30 CV "C

   'i: 0... CV CL cu 20 a: 0 0 C...... QI C 10
   • v 0
   ~ u C1I 0.E 0 u 50 40 ~ 30 C V,

   ?

   20 ~ 10 0 Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture

   ---

   ~--.....:.--"----*------ *------'*---~*~~-----*

   USEPA Minimum Acceptance Criteria (> 15 offspring per surviving female) USEPA Maximum Coeffici.ent of Variation Guidance Criteria(< 42%}, NCDEQ Maximum (< 40%) USEPA Lower PMSD Bound (>13%) USEPA Upper PMSD Bound Acceptance Criteria(< 47%} Control Reproduction, Coefficient of Variation (CV} or Percent Minimum Significant Difference (PMSD) PMSD is the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and is not a minimum acceptance criteria. Central Tendency (mean Control Reproduction, CV or PMSD)

   • 95% Confidence Interval (mean Control Reproduction, CV or PMSD +/- 2 Standard Deviations) ffltWOd bt 5m Sumnt.t t Page 82 of 93

   Entered by: J. Perez Reviewed by: J. Sumner Ceriodaphnia dubia (M_;;;~;;~,..i.i~~ Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture ToKCal Determination Control Reproduction Control Control Reproduction Test (offspring/female) Test number Test date Survival Mean CV MSO PMSO CT 95% Confidence Interval 1%) I offspring/female) (%) 1%) Cl - 2S CT+2S 1 04-04-23 100 29.8 5.9 2.241 7.5 32.0 29.3 34.6 2 OS-02-23 100 31.8 7.2 2.054 6.5 31.9 293 34.5 3 06-06-23 100 31.5 4.8 1.965 6.2 31.9 29,2 34.5 4 06-06-23 100 31.0 4.6 1.573 5.1 31.9 29.2 34.5 5 07-11-23 100 30.0 6.3 l.710 5.7 31.8 29,0 34.5 6 08-08-23 100 32.8 5.9 1..990 6.1 31.7 29,3 34.0 7 09-12-23 100 32.2 5.6 1,717 5.3 31.7 29.3 34.0 8 10*03-23 100 33.l 5.2 1.970 6.0 31.8 29.3 34.2 9 11-07*23 100 31.8 6.6 1.867 5.9 31.8 29.3 34.2 10 12-05-23 100 33.0 6.1 1.849 5.6 31.9 29.4 34.4 11 01-09-24 100 31.S 4.3 1.810 5.7 31.9 29.4 34.4 12 02-06-24 100 32.5 5.3 1.526 4.7 31.9 29.5 34.4 13 03-05-24 100 30.4 8.1 1.973 6.5 31.9 29.3 34.4 14 03-05-24 100 32.6 5.1 1.629 5.0 32.0 29.7 34.4 15 04-09-24 100 32.4 6.2 2.199 6,8 32.0 29.7 34.3 16 05*07-24 100 29.5 6.2 1.707 5.8 31.8 29.3 34.3 17 06-04-24 100 32.5 4.9 1.587 4.9 31.8 29..3 34.2 18 07-09-24 100 32.1 7.6 1.846 5.8 31.7 29.4 34.1 19 08-06-24 100 31.8 6.8 1.827 5,7 31,8 29.5 34.1 20 09-10-24 100 32.4 7.3 1.947 6.0 31.7 29.6 33.9 Note: Control Suniival: USEPA mi'nimum test acceptability criteria~ 80% survival. Control Mean Reproduction= USEPA mTnimum test acceptability criteria~ 15 offsprlng/surviVing female. CV= Coefficient of variation for control* reproduction. usEPA maximum. CV guidance criteria (90"' percentile)< 42%. NCDEQ maximum CV acceptance criteria < 40%, MSD = Minimum significant difference. PMSD = Percent minlmum significant difference, Control Reproduction CV (%, CT 95% Confidence Interval CT - 2S CT +2S 5.4 2.9 7,9 5,5 2.9 8,2 5.4 2.9 8.0 5.5 2.9 8.0 5.6 3.3 7.9 5.5 3.4 7.6 5.5 3.4 7.6 5.5 3.4 7.5 5.6 3.6 7.6 5.5 3.6 7.4 5.5 3.6 7.4 5.6 3.7 7.4 5.8 3.8 7.8 5.7 3.7 7,8 5.7 3.7 7.8 5.8 3.8 7.8 5.7 3.8 7,7 5.9 3.8 7.9 5.9 3.8 7.9 6,0 3.9 8.1 CT 5.6 5.7 5.7 5.7 5.7 5.7 5.7 5.7 5.8 5.7 5.7 5.7 5.8 5.8 5.8 5.8 5.8 5.8 5.8 5.8 PMSD l s -a measure of te~t precision. The PMSD Is the minimum percent difference between the control and treatme.nt that can be declared st-atislically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPI\\ (lo'" percentile) > 13'1,, The lower PMSD bound represents a practical limit to the sensitivity of the test method and is not a minimum acceptance criteria. Upper PMSD bound acceptance crlterfa determined by USEPA (90" percentile)< 47%. CT = Central tendency of the reproduction, CV or PMSD values. S = Standard deviation of the reproduction. CV or PMSD values. Test PMSO (%) 95% Confidence Interval CT-2S CT+2S 4.3 6.9 4.3 7.0 4.5 7.0 4.4 7.0 4.4 7.0 4.4 7.0 4.4 7.0 4.5 7.0 4.5 7,0 4.5 7.0 4.5 7,0 4.4 7.0 4.5 7.1 4.5 7.1 4.4 7.2 4.4 7.2 4.3 7.2 4.5 7.1 4.5 7.1 4.5 7.2 /.."'~**~* ~~-... Page 83 of 93

   Entered by: J. Perez Reviewed by: J. Sumner Page 1 of 6 Sodium Chloride Chronic Reference Toxicant Test {EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia CdNaCICR #: 302 Dilution preparation information: Comments: NaCl Stock INSS number: INSS '"l,."?:)"'l.O Stock preparation: 100 g NaCl/l: Dissolve 50 g NaCl in 500 ml deionized water. Dilution prep (mg/l) 600 800 1000 1200 1400 Stock volume (ml ) 9 12 15 18 21 Diluent volume (ml) 1491 1488 1485 1482 1479 Total volume (ml) 1500 1500 1500 1500 1500 Test organism source: Test randomization and location: Organism age: < 24-hours old Randomizing template color: ~ Ol...b. Date and times 0, 1.--\\ U\\\\'!iD~ Ot'OU Incubator number and shelf L..~ \\ organisms were born between: 00.* 0!>*'\\..-\\ A location: Culture board: Replicate number: i I 2 I 3 I 4 I s I 6 I, 1 s I 9 I 10 Culture board cup number: , I '-\\ I ~ I lo I 10 I II I I~ I I &- "" h.~ Transfer vessel information: pH (S.U.): i. t1.. Temperature (*C): - -- "t'i.o*C. Average transfer volume (ml): <0.25 ml t 'l*ff*'\\.i" Daily renewal: Day Date Test Initiation and feeding,

   • Feeding Batches MHSW Analyst renewal and feeding, or Sele nostrum YWT batch used termination time 0

   09-10-24 011'-i. M-o.-"\\.-\\ o'\\*Ol,, *"l.--\\ Ol\\*04 1.'-lA 1 1 09*11*24 C) "l"'. <":> J l, x{ 2 09-12-24 en~\\ OC\\*O'\\*"-"i)

   it 3

   ~ 09-13-24 o,'-\\,_ }1 4 V 09-14-24 6~'-'."1-nC\\-bl\\*'--\\ B 1,,(

   5.

   09-15*24 Oi~C> \\ ~,\\{ 6 09-16-24 cn~o J, ~ ,u ii 7 09-17-24 01.o\\\\ i1 ~

   • organisms fed daily 100 µL Se/enostrum and 100 µL YWT per replicate using HandyStep repeat pipettor SN 17ES9354.

   Chemical analyses: Parameter Reporting limit Method number Meter Serial number pH O.lS.U. SM 4500-H+ B-2021 Accumet AR20 93312452 Dissolved Oxygen (D.O.) 1.0 mg/l SM 4500-0 G-2021 YSI Model 52CE 08A100271 Conductivity 14.9 µmhos/cm SM 2510 B-2021 Accumet AR20 93312452 Alkalinity 5.0 mg CaC03/L SM 2320 B-2021 Accumet AR20 93312452 Hardness 5.0 mg CaCO,/L SM 2340 C-2021 Not applicable Not applicable Temperature o.1*c SM 25608*2010 Digital Thermometer l°\\Ql,,1,,~\\:i fS Control information: Acceptance criteria Summary of test endpoints: % of Male Adults: i'l7. $20% 7-day LCso (mg/l NaCl) ~ *'-loo % Adults having 3rd Broods: ti'ri7. ~803/4 NOEC (mg/l NaCl) 1000 % Mortality: r,. 7. S20% LOEC (mg/l NaCl) ,,oo Mean Offspring/Female: ~2.'-\\ ~ 15.0 offspring/female ChV (mg/l NaCl} 10'\\S.s %CV: ""\\. ~ 1. <40.03/4 IC2s (mg/L NaCl) 1\\0$.l SOP AT14-Revision 6-Exhibit AT14.1 Page 84 of 93

   Entered by: J. Perez Reviewed by: J. Sumner Species: Ceriodaphnia dubia CONTROL Day 1 1 Young produced t"i Adult mortality \\ 2 Young produced (') Adult mortality 3 Young produced ("\\ Adult mortality L-4 Young produced c; Adult mortality 5 Young produced \\ t'\\ Adult mortality L 6 Young produced 0 Adult mortality L 7 Young produced '~ Total young produced ~ Final Adult Mortality l_ X for 3n1 Broods ~ 2 0 \\..... 0 (') ~ \\~ b I'-\\ ~b ',.(_ Page 2 of 6 CdNaCICR #: 302 Survival and Reproduction Data Replicate number 3 4 5 6 7 8 9 10 (') D 0 (') 0 0 0 0 L \\_ '- L \\.._ (') D 0 {) D ('.) 0 0 ~ '- ('\\ [) {") {) 6 0 C 0 L.. '-- \\_ '-- L L L L ~ '--\\ ~ '--\\ '.\\ c.. '-\\ '-l \\._ \\\\ \\\\,, *~ \\\\ \\1_ \\ (J \\~ L. \\..... \\..... '- \\._ '- {) ('I C b ('\\ C ('\\ C) \\...... \\..... '- \\..._ L I\\ IC\\ l f \\ ':>,, \\ t:\\ \\~,~ ~'!) ~--\\ ~'-\\ 1., l)'l,.. ~, ~~ ~""L. \\...... L L ~ )( '-.C. V )<.. '.>C- >,c"' ""')c-Note: Adult mortallty (La: 1!1J*, 0 ;. dHd), SS ; spUt brood (slnglt1 brood ~plir between two days). CO,; any o\\11:r(0ffsprfn1 carried over wfth adult durtnc tninsfer). Concentration: % Mortality: I 01. Mean Offspring/Female: I ~'L<{ 600 mg NaCI/L Survival and Reproduction Data Replicate number Day 1 2 3 4 s 6 7 8 9 10 1 Young produced t) 0 0 C) (\\ D 0 0 0 n Adult mortality \\...- L L L \\..._ L L \\.._ 2 Young produced ('-\\ l'\\ () ('} t) 0 D D a Adult mortality \\. L L- '- L \\._. L 3 Young produced () 0 f) 0 (") 0 0 D Q 0 Adult mortality L L. L L L L L \\,_ '- 4 Young produced 'i 4 '-\\ ~ ~ ~ ~ '-\\ '-\\ Adult mortality \\..- '- \\.... L. '- L L \\._ 5 Young produced I \\ *~ l() l\\ \\\\ It),,_ I "L I C)

   1)

   Adult mortality \\._ \\_ \\_ \\,_ '- \\._ \\._ '-- 6 Young produced 0 t\\ 0 0 0 () 0 0 n 0 Adult mortality \\.._ L \\_ '- '- L L 7 Young produced \\ <<:, '6 ly Is Ho ,s If' lk ,1 Total young produced ~\\ ~, ~,_ '!)'\\ '2~ ~\\ ~""2. !)$ ~1. ~\\,, Final Adult Mortality l- ~ '- L \\.,_ l Not,: Adult mort*lltv fl= !Iv*. 0

   • du d), SB= split brood (slncl* brood,pht betw.. n two doys), CO= urry ovor (cffspnns corned over with *dult durlns tnnsfu).

   Concentration: % Mortality:

   01.

   Mean Offspring/Female: 32.7 % Reduction from Control: -o,q 7. SOP AT14-Revision 6-Exhibit AT14.1 Page 85 of 93

   Entered by: J. Perez Reviewed by: J. Sumner Species: Ceriodaphnia dubia 800 mg NaCI/L Day 1 1 Young produced n Adult mortality L 2 Young produced t\\ Adult mortality L-3 Young produced (') Adult mortality L-4 Young produced ~ Adult mortality L. s Young produced \\ "- Adult mortality 6 Young produced 0 Adult mortality L 7 Young produced I 5> Total young produced

   ."'2..

   Final Adult Mortality L.. z 0 \\. () r--.. L ~ \\ 0 0 L 1'- ~\\. Survival and Reproduction Data Replicate number 3 4 s 6 7 0 0 0 D 0 l- \\..- \\__. L. L. D 0 D (', D \\...... (') t) C) {) () L. '- L. '- ~ ~ ~ '-\\ '-\\ L. '~ \\~ \\"\\.. \\\\ \\\\ '\\._ '- '- '\\,_ n 0 0 0 0 L L L- \\...... L ' i I\\. I°' \\ '5,1 r ~;*.,... ~ ~,_ ~ I..\\ ~I) ~,_ c=... L.. Page 3 of 6 CdNaCICR #: 302 8 9 10 0 0 0 L L. '- 0 (\\ () \\._ \\._ '- (') t'I 0 L L '-\\ ~ ~ \\..... '- \\ \\ 11-~- I{) L L 0 0 0 L \\.. L lb \\\\c \\'\\ '2&

   ,'\\, ~:,

   L.. Not,: Adu" mort*lily (l = live, 0 : dndl, SB= 1plll brood (slnil* brood split botwun two d1y1), CO* carry over (offsprfoc cornod over with,dult durlnc tn,nsftrl, Concentration: % Mortality: () 7. Mean Offspring/Female: ,3\\ ~ % Reduction from Control: ,~ 7. 1000 mg NaCI/L Survival and Reproduction Data Replicate number Day 1 2 3 4 s 6 7 8 9 10 1 Young produced 0 {) {) f\\ 6 /) 0 0 0 0 Adult mortality \\_ L-L L L L L. L L L 2 Young produced /) () 0 (\\ 0 () 0 0 0 D Adult mortality L \\..._ \\._ \\...... 3 Young produced 0 () {) n () () 0 0 D 0 Adult mortality L L L. '- L L. '- '- 4 Young produced '-l '4. \\. '-\\ '-\\. ~ '-l '-\\ y Adult mortality \\.._ '- L. s Young produced IQ 10 IL. IU \\ "\\...

   • O 10

   \\,.__ ,L

   11...

   Adult mortality L L L L... \\._ L 6 Young produced {) (\\ ri /) 0 0 0 0 0 0 Adult mortality L L L L L \\._ L L.. L L 7 Young produced ~~ \\\\ ' & l \\ I '-t 17 I~ I 1.\\ 11 Total young produced "\\.~ ~~ 1,\\:, ~~ ~~ 1.., ~\\ "'\\.." ~b '?,~ Final Adult Mortality \\.._... '- L \\.._... \\.._... Nott: Ad1.1\\l mon11lty {L =- lrve, O; dud), S8 c: Jp\\ft brood jslncle brood split between two d1ys)1 CO~ carry ovu {off1prin1 c.arri*d over with 1dult durinc transf1r). Concentration: % Mortality:

   07.

   Mean Offspring/Female: 3 1. lc, % Reduction from Control: 2.. s 1. SOP AT14-Revision 6-Exhibit AT14_l Page 86 of 93

   Entered by: J. Perez Reviewed by: J. Sumner Species: Ceriodaphnia dubia 1200 mg NaCI/L Day 1 1 Young produced ("') Adult mortality L-2 Young produced (J Adult mortality t 3 Young produced ('\\ Adult mortality 4 Young produced ~ Adult mortality s Young produced '\\ Adult mortality 6 Young produced D Adult mortality 7 Young produced l.. Total young produced lg-Final Adult Mortality \\._ 2 C) \\.._ n 0 \\.._ '-\\ ,o (') L. 'i 1 t \\.._ Survival and Reproduction Data Replicate number 3 4 5 6 7 {"") 0 0 0 () L- \\__ L. \\_ () ('\\ f) ('\\ (") \\.._. '- 0 0 0 0 0 L- \\...- L- ~ "' ~ '-\\. 10 i: I \\ s I 0 L \\..... '\\..._ {) n n C) 0 4 c; 1 ~ s li

   1. \\

   i, Ii \\..__ \\_ L.. Page 4 of 6 CdNaCICR #: 302 8 9 10 ('\\ 0 0 L L L t\\ () 0 L- '- '-- 0 0 6 L \\._ '-\\ Y. ~ \\.- \\..... 10 \\o ~ L.. L 0 0 a L... '- \\._ 1,_ y e-tf-If If \\..... Nore: Adoll rnortallty (l = llve, 0 s dud), SB: spilt brood (.sf,,sl1 brood split between two d1ys), CO: urry onr (offspril'II carrf1d ewer with adult durin1 u11nsf~r). Concentration: % Mortality:

   01.

   Mean Offspring/Female: \\i, C) % Reduction from Control: '-1'\\.'-\\ 1. 1400 mg NaCI/L Survival and Reproduction Data Replicate number Day 1 2 3 4 s 6 7 8 9 10 1 Young produced n () 0 0 0 0 {) () n 0 Adult mortality L L \\.._.. L L.. L__ L L L 2 Young produced 0 b 0 [) 0 D r-.... {) () r, Adult mortality L L.. \\.._ L.. '- \\.... l 3 Young produced (') ('\\ /"i 0 {') 6 D 0 C D Adult mortality L L.. L.. L L L L L... 4 Young produced "'L \\ ~ ~ \\ \\ \\ Adult mortality \\ l..- '\\_. \\_ \\_ \\..... '- ~ s Young produced 0 0 0 0 Q 0 0 0 0 0 Adult mortality L \\.._. \\_. ~ L \\.... \\_ \\_ 6 Young produced 0 \\ 0 0 0 0 0 (, I n Adult mortality L L L \\.._ L \\...... \\_ L \\.... \\....... 7 Young produced {'\\ 0 0 0 0 0 () 0 CJ 0 Total young produced 'L 1-1.. ~ I l.. J Final Adult Mortality L. '- L. \\...... '- L \\...... '- L-Not*: Ad Ult mort11ity (L:. live, 0: dHd), SS:;. sp!ft b,ood (1in,1le brood spilt between two days), CO= car,v over {offsprin1 curied over with adult durln& tRnsfer), Concentration: % Mortality:

   07.

   Mean Offspring/Female: 2.0 % Reduction from Control: -=t~ t 1. SOP AT14-Revision 6-Exhib[t AT14.1 Page 87 of 93

   Entered by: J. Perez Reviewed by: J. Sumner Verification of Ceriodaphnia Reproduction Totals Environmental Testing Solutlons, Inc. Control 1000 mg NaCI/ L Day Replicate number Total 1 2 3 4 s 6 7 8 9 10 Day Replicate number Total 1 2 3 4 s 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 s 3 s 4 s 4 4 6 4 4 44 4 4 4 6 4 4 s 4 4 4 4 43 s 10 13 1.1 1.1 11 10 11 12 10 13 112 5 10 10 12 10 12 10 10 12 12 12 110 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 15 14 17 19 18 15 17 19 19 15 168 7 15 19 18 19 17 14 17 13 14 17 163 Total 30 30 33 34 34 29 32 37 33 32 32.4 Total 29 33 36 33 33 29 31 29 30 33 316 600 mg NaCI/ L 1200 mg NaCI/L Day Replicate number Total 1

   2.

   3 4 s 6 7 8 9 10 Day Replicate number Total 1 2 3 4 s 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 s 6 4 4 3 5 s 5 4 4 45 4 3 4 4 4 3 4 2 4 4 4 36 s 11 13 10 1.1 1.1 10 12 12 10 13 113 5 9 10 10 8 11 5 10 12 10 6 91 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 15 18 18 19 15 16 15 18 18 17 169 7 6 4 4 s 7 8 5 2 4 8 53 Total 31 37 32 34 29 31

   32.

   35 32 34 32.7 Total 18 18 18 17 21 17 17 18 18 18 180 800 mg NaCI/L 1400 mg NaCI/L Day Replicate number Total 1

   2.

   3 4 s 6 7 8 9 10 Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 5 4 3 4 4 4 6 4 44 4 2 1 2 2 3 2 1 1 1 3 18 s 12 10 10 12 12 11 11 11 12 10 111 5 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 6 0 l 0 0 0 0 0 0 l 0 2 7 15 16 18 16 19 15 17 13 16 19 164 7 0 0 0 0 0 0 0 0 0 0 0 Total 32 31 33 32 34 30 32 28 34 33 319 Total 2 2 2 2 3 2 1 1 2 3 20 ~ ~l" Page 88 of 93

   Entered by: J. Perez Reviewed by: J. Sumner Environmental Testing Solutions, Inc. Concentration (mg/LNaCI} 1 2 Control 30 30 600 31 37 800 32 31 1000 29 33 1200 18 18 1400 2 2 Ounnett's MSO value: 1.947 PMSO: 6.0 3 33 32 33 36 18 2 4 34 34 32 33 17 2 MSD= PMSD= Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Stat istical Analyses Replicate number Survival s 6 7 8 9 10 (%) 34 29 32 37 33 32 100 29 31 32 35 32 34 100 .34 30 32 28 34 33 100 33 29 31 29 30 33 100 21 17 17 18 18 18 100 3 2 1 1 2 3 100 Min1mum Significant Difference Percent Minlmum Significant Difference Test number: CdNaCICR #302 Test dates: September 10-17, 2024 Average reproduction Coefficient of Percent-reduction (offspring/female) variation (%) from control (%) 32.4 7.3 Not applitable 32,7 7.1 -0.9 31.9 5.8 1.5 3L6 7.5 2.5 18.0 6.4 44.4 2.0 33.3 93.8 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant In a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10 11' percentile) = 13%. Upper PMSD bound determined by USE PA (901~ percentile)= 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WIT lnterlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). The lower PMSD bound represents a practical limit to the sensitivity of the test method and is not a minimum acceptance criteria. USE PA. 2001a, 2001b, Final Report: lnterlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendlx. EPA-821-8-01-004 and EPA-821-8-01-005. US Environmental Protection Agency, Clncinnati, OH. ii!""'"'~* ,,:a-.. -;r Page 89 of 93

   Entered by: J. Perez Reviewed by: J. Sumner Statistical Analyses \\ --------- £nvl,onm*nu11 Testing.Sol~Uons. In<~ Cerlbdal)hnla Survtval11nd Reproduc:tlOn Teat-Reproduction 1siart Date: 9/10/2024 Test ID: CdNaCICR Sample ID: REF-Ref Toldcant End Date: 9117/2024 lab ID: ETS-En-.ir. Testing Sol. Sample Type. NACL-Sodium chloride I sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc:-mg/L 1 2 3 4 5 6 1 8 9 10 D-Contrcl 30.000 30.000 33.000 34,000 34,000 29.000 32.000 37.000 33,000 32.000 600 31.000 37.000 32.000 34,000 29.000 31.000 32.000 35.000 32.000 34.000 800 32.000 31,000 33.000 32.000 34.000 30,000 32.000 28.000 34.000 33.000 1000 29.000 33.000 36.000 33.000 33.000 29.000 31.000 29.000 30.000 33.000 I 1200 18.000 18,000 18.000 17.000 21.000 17.000 17.000 18.000 18.000 18.000 1400 2.000 2.000 2.000 2.000 3.000 2.000 1.000 1.000 2.000 3.000 I Transform: UntransfOt"med Rank 1-Tlllfed Isotonic Cone-ma/I. Mean N-Mean Mean Min Mall CV% N Sum Critical Mean N-Mean D-Control 32.400 1.0000 32.400 29.000 37.000 7.304 10 32.550 1.0000 600 32.700 1.0093 32,700 29.000 37,000 7.070 10 108.00 75.00 32.550 1.0000 800 31.900 0,9846 31.900 28.000 34.000 5.809 10 100.00 75.00 31,900 0.9800 1000 3 1.600 0.9753 31.600 29.000 36.000 7.489 10 94.50 75.00 31.600 0.9708

   • 1200 18.000 0.5556 18.000 i7.000 21.000 6.415 10 55.00 75.00 18.000 0.5530
   • 1400 2.000 0.0617 2.000 L000 3.000 33.333 10 55.00 75.00 2.000 0.0614 Auxiliary Tests Statistic Crltlcal Skew Kurt I

   Kolmogorov D Test Indicates normal distribution (p > 0.01) 1.00513 1,035 0.26346 0.5049 Bartlett's Test indicates unequal variances (p = 5.88E-03) 16.364 15.0863 I HYl>OthHls Test (1-tall, 0.06) NOEC LOEC ChV TU Steel's Many.One Rank Test 1000 1200 1095.45 Treatments vs D-Control Unear loterpolallon (200 Resamplesl ,Point mgil so 95%CL Skew IC05 1009.96 93.311 719.766 1022.95 -2.5346 IC10 1033.9 11.2597 1005,33 1045.91 -1 0407 IC15 1057.83 9.73901 1032.19 1069.24

   • 0.5890 1,0 IC20 1081.76 8.79896 1057.96 1092.75

   -0.5443 0,9 IC25 1105.7 7.96272 1083.74 1116.55 -0.4675 IC40 1177.5 6.46636 1163.17 1188.98 0.0074 0,8 ICS0 1221.56 4.81147 1211.46 1229.95 -0.01 43 0.7 0.6 .. i 0.5 .,, OA &! 0.3 0.2 0.1 0,0 -0, 1 0 600 1000 1500 Dose mg/l Dose-Response Plot 0 35 I T T 30 1 I C 25 ,g l 20

   a.

   &! 15 10 5 - 0 ~ ~ ~ ~ ~ C ~ Page 90 of 93

   Entered by: J. Perez Reviewed by: J. Sumner Statistical Analyses E'livfronmen-tal Testing SoluUoos, Inc. Used for PMSD calculation only. Cerlodaphnla Survival and Reproduction Test-Reproduction Start Date: 9/10/2024 Test ID: CdNaCICR Sample ID: REF-RefToxicant End Date: 9/17/2024 Lab ID: ETS-Env!r. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date~ Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-rng/L 1 2 3 4 5 6 7 8 9 10 D-Control 30.000 30.000 33.000 34.000 34.000 29.000 32.000 37.000 33.000 32.000 600 31.000 37.000 32.000 34.000 29.000 31.000 32.000 35.000 32.000 34.000 800 32.000 31.000 33.000 32.000 34.000 30.000 32.000 28.000 34.000 33.000 1000 29.000 33.000 36.000 33.000 33.000 29.000 31.000 29.000 30.000 33.000 1200 18.000 18.000 18.000 17.000 21.000 17.000 17.000 18.000 18.000 18.000 1400 2.000 2.000 2.000 2.000 3.000 2.000 1.000 1.000 2.000 3.000 Transform: Untransformed 1-Talled Conc,mgll. Mean N-Mean Mean Min Malt CV% N t-Stat Crltlcal MSD D-Control 32.400 1.0000 32.400 29.000 37.000 7.304 10 600 32.700 1.0093 32.700 29.000 37.000 7.070 10 -0.352 2.287 '1.947 800 31.900 0.9846 31.900 28.000 34.000 5.809 10 0.587 2.287 1.947 1000 31.600 0.9753 31.600 29.000 36.000 7.489 10 0.939 2.287 1.947

   • 1200 18.000 0.5556 18.000 17.000 21.000 6.415 10 16,910 2.287 1.947 "1400 2.000 0.0617 2.000 1.000 3.000 33.333 10 35.698 2.287 1.947 Auxlllary Tests Statistic Crttlcal Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 1.00513 1.035 0.26346 0.5049 Bartlett's Test indicates unequal variances (p = 5.88E-03) 16.364 15.0863 Hypothesis Test (1-taU, 0.05)

   NOEC LOEC ChV TU MSDo MSDp MSB MSE F-Prob df Dunnett's Test 1000 12.00 1095.45 1.94728 0.0601 1565.79 3.62593 3.1E42 5, 54 Treatments vs D-Control Dose-Response Plot 40 35 T I

   ---

   **---------r-------*--* -----------------------

   1-tail, 0,05 level 30 of significance 5 25 u ~ 20

   a.

   &! 15 10

   • 5 0

   g 8

   q 0 § g ~ 8 ? 8 ~ Page 91 of 93

   Entered by: J. Perez Reviewed by: J. Sumner Page 5 of 6 Species: Ceriodaphnia dubia CdNaCICR #: 302 Daily Chemistry: Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet. Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 0 1 2 Analyst v( $,ufv ,,.,, vt, 1£.,,,,,A, ~,,.,,, v\\., ~ Concentration Parameter pH (S.U.) '..J,)5 7.35 7."1-9

   7. 2'2 7-32.

   '1.S?, Dissolved oxygen (},) '1e 16 l)t? ~I) ~B (mg/L) Conductivity 2trg ~3o 3/8 CONTROL, MHSW (µmhos/cm) Alkalinity ~"\\ I"- 1,,J.. - -~ (mg CaCO,/l) ~~* L - Hardness 8'1 ~ 4'<<~ ~ (mg CaCO,/L) I Temperature (*C) "\\S-D "\\.',. 'L-

   i. '-\\. \\

   ~\\-~ "\\,"(,'\\ "'\\.~.'\\... pH (S.U.) 'f,B 7,q.q. ~4-<J 7.tf / 7, 'I I '1.51 Dissolved oxygen 11 /}£> ~a '113 '12' f,) 600 mg NaCI/L (mg/L) Conductivity l~oo (µmhos/cm) t '-t-'lo /tf7o Temperature (*C) ~-\\ '\\.'i,. Q -u.. 0 7.'i-'1- "\\S. 0 "\\.~. o pH (S.U.) /-+.+-1

   7. i./-6 7,50

   7. 4-5 7, 'f Cj

   '1-.~ Dissolved oxygen '4. ~ ~-1 98 ~.e 1),,6 --:},(o (mg/L) 800 mg NaCI/L Conductivity 11:l) 1871'; l8-so (µmhos/cm) Temperature (*c) "'\\S.o "'\\S-~ ~.o "\\~. C '\\S, 0 '\\.S. '\\- pH (S.U.) '+-tv 7 4-C, 7.51 7,t/7 7,lfl/- r t,S'lo Dissolved oxygen -':};(3 ~') I:)~ /)-2 118 1-C... 1000 mg NaCI/L (mg/L) Conductivity 2lto (µmhos/cm) "Z. 1-<fo 2,2-2-0 Temperature (*CJ '-'. l) i.~.\\ '\\.S.l) '\\S*Zl ,S,0 "\\S. \\ pH (S.U.} 't.1-ll 7 -"c- ~ 7.f;) 7, 'I 7 7.50 '1-,Slo Dissolved oxygen l~~ '1A l:)g I'.).e ~B 1.~ (mg/l) 1200 mg NaCI/L Conductivity (µrnhos/cm) ~"n-o 2£10 2.S?o Temperature ('C) "'1/4 -0 '-S*~ ~-D

   l. 'l* ~

   1.'i* 0 "\\."'-°\\ pH (S.U.) /~,:}1 7,?17 7.St../- 750

   7. sz.

   4,'S1-- Dissolved oxygen ~.D ().0 1.9 ~~ ',.{j -:Y,0 1400 mg NaCI/L (mg/L) Conductivity (µmhos/cm) 9-0~0

   1. qp, (]

   2-CJ'?o Temperature (*C) 'l<;.D '\\..<;. L ls, n 1.S-\\ ,s.o 1A,"\\ Initial Final Initial Final Initial Final SOP AT14-Revision 6-Exhibit AT14.l Page 92 of 93

   Entered by: J. Perez Reviewed by: J. Sumner Page 6 of 6 Species: Ceriodaphnia dubia CdNaCtCR #: 302 Day (Analyst identified for each day, performed pH, 0.0. and conductivity measurements only.) 3 4 5 6 Analyst ~ k.L CJ..~ (!.,c, I' ().,c.,1 1f;4-1 G/"? ~- I Concentration Parameter I pH (S.U.) , ~.ti 1.. 'J.. a-:i.

   J4~

   ~.41 +.Lll 7;j/ 7 s /

   7. ~t:)

   Dissolved oxygen 1,,. 157

   }/}

   1.,g

   ':l. 'iS , <...,.~ ,. I.a 7. '\\ {mg/L) ~ ,:~,t!,, -~ Conductivity CONTROL, MHSW {µmhos/cm) .3()...f . ~. 311.., '. 3~ 3io ..J Alkalinity

   • =--
   • V-\\

   (mg CaC0 1/L) ~-...... ~ "f Hardness ,...~. ~ ~o f'lri' 81 (mg CaCOJ/L) ~- Temperature ( 0C) "'\\.-l'\\ "'-". "'\\... '-'\\ -'\\ ""\\.,~. ; -"\\.'-\\. '\\ "\\5.1.- -i.'\\. I pH (S.U.) 't~ 1,,51#

   1.41

   J.~~

   J.1.1-3 7,60

   ?. t, C,

   • 7.6n Dissolved oxygen 1-."t-1,'I 1-i

   J.'\\.

   +~ \\* J,., &. C> {mg/L) 600 mg NaCI/L Conductivity (µmhos/cm) (3m \\'430 [I 1430 L~-z_o Temperature {0CJ "\\{.. 0 '"'--'--A "\\..S. 0 " ~--.\\ '\\.s.o "\\..S.O ,.i, '\\ ~~- ~ pH (S.U.) r.i.o l.55

   J.,54

   =l-,u5 ':l,.1.t 1' 7./4~ 7,?I 7-l~I Dissolved oxygen ~-1

   1-. °I

   +-~ ~,'l +.'1 "'\\.~ ,.1 ~-0 (mg/l) 800 mg NaCI/ L Conductivity ,11.o (µmhos/cm) lioo r1-qo lf350 Temperature (*CJ ~""*" 1.C..-\\ "\\.S,0 ""\\..S.,_ '\\S,'D "\\5. ~ 1.~- 0 '\\S* '\\... pH(S.U.) "*t-=tO

   t.5c.,

   1..55 ':J,\\.(l, l,\\, f' 177~ .., -?~ ~,7 ..... '7.6 ~ Dissolved oxygen f ,(,o ,.'\\ (mg/L) t-1'1 -:J. :,

   ui

   -::f }\\ 1000 mg NaCI/L Conductivity 2aio I (µmhos/cm) ~ll.,O ~\\=\\0 -z_L 9o Temperature ( 0CJ "\\.."\\. ~ '-~-"\\.- "\\.'.. 0 '\\S."l.. u,. D "LS- "\\... ~ - 0 \\.4i. '- pH (S.U.) l},10 1-.511

   i,vv

   =l." ~ 77q 7.Bo 7.6'f Dissolved oxygen i,<a '\\..O ~.t) 1- !'I i-o

   7. &-

   ,.~,., (mg/L) 1200 mg NaCI/L Conductivity ~~?ln (µmhos/cm) ~'1-:1.n JS10 2.'5So Temperature (0C) "L'\\.1 "'\\..C.. '\\... "\\..~.o \\. '>* ""L '1.*\\. '\\

   u. J.

   "\\.~. D '\\.\\.) pH (S.U.) /'\\.~I

   J.5S

   J:5(,,

   '.lk'5

   -10 7,71 7.g I

   7. /24-Dissolved oxygen

   -::};~ i.o ~ -0 -=1}'1 j.D ,."1 7. ~ ,.~ (mg/l ) 1400 mg NaCI/L Conductivity I (µmhos/cm) ZBrJ> ~ftx:> J'i 1() 2.t}3r> Temperature (°C) ,.~.o "'-~ - ~ ~'\\.-"\\ "\\c;.. "L 1....'-\\." "lS. "l 1..1(. " '\\S.,l Initial Final Initial Final Initial Final Initial Final SOP AT14-Revision 6-Exhibit AT14.1 Page 93 of 93}}