Text

Discharge Monitoring Report, October 2020, Part 2 of 3
	ML20350B706
Person / Time
Site:	Sequoyah
Issue date:	11/12/2020
From:	; Tennessee Valley Authority
To:	; Office of Nuclear Reactor Regulation
Shared Package
ML20350B703	List: ML20350B704; ML20350B706; ML20350B707;
References
	Download: ML20350B706 (28)
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{{#Wiki_filter:BIOMONJTORING CHAIN OF CUSTODY RECORD P:.igc _I_ of _ I_ Client: TVA Environmental Testing Solulion, Inc. Delivered Oy (Circle One): Proj.:cl Name: Sequoyah NP Toxicity 351 Depot Street: FedGx UPS llus Client - - P.O. Number: NIA Asheville, NC

'O~r (specify): S~.. :c.

Facility Sampled: Sequoyah NP 28801 General Comments: 101 Boule Comp@ '-' 7<;'-ro NPDES Number: TN0026450 Phone: 828-350-9364 i/. 11 /-.,*.

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Whole Effluent Sample Receipt Log page _!],:o 'Sample temperature performed using 11erfied General Use Thermometer SN: ------'\\_""\\..:....;:0_'1_<.:._~~'\\_1..;..;:0 _ _ Date Time Received Received

sample Project Sample Sample name and description State Comments Received Received by from Temp. (°C}

number number 10-09-20 0950 K. Keenan Fed - Ex 3.6 15444 201009.01 Belews Creek SS - 006A NC 10-09-20 0950 K. Keenan Fed - Ex 2.6 15445 201009.02 franklinton WTP NC 10-09-20 0950 K. Keenan Fed - Ex 2.6 15446 201009.03 Goldsboro WRF NC 10-09-20 0950 K. Keenan Fed - Ex 3.0 15447 201009.04 HamletWWTP NC 10-09-20 0950 K. Keenan Fed - Ex 3.1 15448 201009.OS IP - Rieglewood NC 10-09-20 0950 K. Keenan Fed - Ex 4.5 15449 201009.06 MonroeWTP NC 10-09-20 0950 K.Keenan Fed - Ex 3.9 15450 201009.07 Perdue, Inc. NC 10-09-20 0950 K. Keenan Fed. Ex 4.1 15451 201009.08 Ponzer WTP NC 10-09-20 0950 K.Keenan Fed-Ex 4.2 15452 201009.09 Buck CTCC - 007 NC 10-09-20 0950 K. Keenan Fed* Ex 3.7 15468 201009. 10 Oriental WTP NC 10-09-20 1305 J. Sumner TVA Courier 1.7,2.. 1 15420 201009.11 TVA - Sequoyah Nuclear, Outfall 101 TN 10-09-20 1305 J. Sumner TVA Courier 2.0 15420 201009. 12 TVA - Sequoyah Nuclear, Outfall 101-Intake TN 10-09-20 1414 J. Sumner TVA Courier 2.2, 2.0 15419 201009. 13 TVA - Watts Bar Nuclear, Outfall 101 TN 10-09-20 1414 J. Sumner TVA Courier 1.6 1S419 201009.14 TVA - Watts Bar Nuclear, Outfall 101 - Intake TN 10-09-20 1414 J. Sumner TVA Courier 1.9,1.9 15419 201009.15 TVA - Watts Bar Nuclear, Outfall 113 TN 10-09-20 1414 J. Sumner TVA Courier 1.5 15419 201009.16 TVA - Watts Bar Nuclear, Outfall 113

Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12 26 of 90

Page 1 of 7 Envlronme-nt.:a1Te1tJng Solu'tlgris. Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales prome/as Client: TVA, Sequoyah Nuclear Plant NPDES #: TN 0026450 Project#: Dilution preparation: Dilution prep (%) 17.25 34.S 69 Effluent volume (mL) 345 690 13&0 Diluent volume (ml) 1655 1310 620 Total volume (ml ) 2000 2000 2000 Test organism information: Organism source; In-house culture Age: < 24-hours old Spawn date: O'Vl.'\\-'l.O 1~-0~*'1..o 1'4'\\ 'O Hatch dates and times: lb* QI..* 'Lt) Transfer vessel information: pH {S.U.) = \\* YL Temperature (*CJ = Average tran~fer volume (ml): .; 0,25 ml 84.5 100 1690 2000 .HO 0 2000 2000 "Tb oS-\\Q 1.-\\-S County: Hamilton Outfall #: 101 Treatment: UV {Each concentration was UV-treated for 2-minutes to remove pathogenic interferences.) Sample was not aerated or treated unless otherwise noted on this form. Sample was warmed to 25.0 +/- 1.o*c in a warm water bath and then diluted to the test concentrations with moderately hard synthetic water (MHSW), Test information: Randomizing template: -R, Je_ Incubator number , L and shelf location: Artemio CHM number: CHM1149 Drying Information for weight determination; Date/ Time in oven: 10..l'l,-..- odo.S

Initial oven temperature:

'-" *[ Date/ Time out of oven: 11\\ *t\\l*l.n oPhc;:

Final oven temperoture:

,_,,. (_ Total drying time: 7A-l-\\oJ"1.S

60°( Oven, Thermometer SN: 14-98585 Daily feeding and renewal information:

Day Date Morning feeding Afternoon feeding Test initiation, renewal, Sample MHSW or termination J'IUmbers used batch used Tlme Analyst Time Analyst Time Analyst Outfall 101 Intake 0 10-06-20 oS'-.> \\A \\\\i.\\S o.e1.~ \\h "1..01005. OS. "l.01oos.ot.. cr>i*u-*"lo~ IA 1 10-07-20 ~A~ I'- ok-1:6 I'- J., l, l C\\S "l;,IJ ll!>Cl LI 2 10-0S-20 o-l ID ID I() lO ID ,o 10 IV ID IO ID 2 /\\) l () ' ti I CJ

10 IQ lD
/ \\) ID I\\) ID I D I 3 ,o 10 10 /0,o 10 I () ID ( () /0 JO 10 4 I() / () 10 IC) 10 I() IQ /() I() IO to 'D 5 {f::J I c:, ID ' {) 10 I {) IO 10 I\\) 10 ro 10 6 ,o 10 10 10 / l) 10 / () IO ID IC) IC) 10 7 ,o 10 IQ ID /{) ID 'D ID,a (Q 10 D
A = Pan weight (mg)
~ 11-\\ v..° b°'~ q,I\\. ~ (\\ /\\ Tray color code: Mq~e,I')-\\-~ (9 \\ ~~ 00 ~ 'b ,__"? ~ ~ C(:, b"'J ~ Analyst: ()° ~) 0 0 rs c; ()' 0 o* 0 () o* Average weight per initial Percent number of larvae (mg) reduction o.1 °I~ o.1s4 s.-i,_ O.71o<\\ 3,. 1 1. from control{%)
Weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41S20.
Comment codes: c = clear, d = dead, fg = fungus, k = kilfed, m ::: missing, sk = sick, s111 = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. I Comment,: I 28 of 90 SOP AT20-Revision 6-Exhibit AT20.3
Page 3 of 7 Er1vl,onment,1JTestlng Solu1lon.s. Ir,(. Species: Pimephales prome/os Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 10-06-20 Survival and Growth Data Day 69% 84.5% 100% M N 0 p Q R s T u V w X 0 10 10 10 10 10 10 10 10 10 10 10 10 1 /'\\) 1() I~ /\\) {Q I{) ID 10 (Q 10 10 10 2 I C) IQ I () ,o I l) / t> I {) It) 10 10 10 / \\) 3 l o I Q 10 I D JO,o ID '0 10 lO,o tD 4 lo 10 10 I D I() IC) 1 '() I O I Q 10 to 10 s I '() 10 10 I D
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,o 'O (0 I Q IL) 6 IO 10 IC) 10 10 ID (0 ID IO ( \\) 10 10 7 I() ,~ '() , 0 'D
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IO 10 I() 10 I O I [)
A :: Pan weight (mg) 6~
'>)'").. "°' '-9':) °'i Tray color code: MGl~ V\\~Q,, "-°' ~ \\'J..?v.. \\,\\\\ ~ui ~Cl,, 'l:>9, Analyst: :cs \\W* ,\\,\\. \\0* \\~o \\V.* ,v... ,v._.,~. ,v;.. \\lP* Date: Oi, 30 - ~
e.. Pan + larvae weight (mg)
,.,,qs 1.>:*-l'-\\ 1.1. 11:, 1.1..H Analy~t: ~ 1"\\-'!.'\\ 1,.1..1/ L n.~ 1.1.'ilo 1.\\-~ -u.(\\a i1.'o6 l.'!.AS Date: I 0-\\"\\*'"\\.0 C = Larvae weight (mgJ = B - A J.10,.... ~,. le, 1,. ~' v\\\\ -,AS '"\\.. \\1..,.n,.1oS 7.5 \\ 7.1.1.. 1.s1 Analyst: 'i Weight per initial number of larvae (mg) = C / Initial num ber of larvae 0 ~
,..S 0--
1{' ,'v s' '\\,'\\, ,....s" ~
P
~ /\\."' I\\ A I\\ I\\ I'\\ Analyst: ,i 0 C)* t",
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()" 0 o* o* ti* I.Y C)* Average weight per initial Percent number of larvae (mg) reductio n ('.). "1~ 1.. (.. \\?. from co ntrol 0.11.'\\ ~-~7. 0. -,'-\\'\\ b, ~ 7. (%)
Weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520.
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg= unusually large, d&r = decanted and returned, w = wounded. I Commentso 29 of 90 SOP AT20-Revision 6-Exhibit AT20.3
Page 4 of 7 EnvhonrnC'nt;,ITullng Solutioo,,, Jn" Species: Pimephales promelas Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 10-06-20 Survival and Growth Data Day 100% Intake Control, Non-treated y 2 AA BB cc DD EE FF 0 10 10 10 10 10 10 10 10 1 la ID I\\) IQ ID I 0 I () lV 2 I{) 1 0 I () I() \\ C) 10 ID ID 3 lo IQ I o IO ID ( 0 10 10 4 lo 11.) IO 10 IQ 10 IO IO 5 10 lo 10 10 lo IQ 10 10 6 ID ID IO IO ID / C) , C) 10 7 q',t {(:) \\ () ,o 10 ID 10 10
A = Pan weight (mg) 4.'a-
°' ,{\\ Tray color code: ('(\\%er, i'Q, w\\ \\\\9?-0 0 \\6!' ,p I\\'\\ Analyst: '~ \\\\,\\* ,v-..,~. \\\\\\ *,"'* \\Y-.* Date: O:l,30 -~
a= Pan+ Larvae weight ~g)
Analyst: L(l.o.s 1.'l.-~ 1,1.S'l.. 1.1.IS u :1-t 1.1.- I 0 1.~.--10 i1.1.-\\ Date: j()*\\'\\*'U) C" Larvae weight (mg) = B - A lo.~-\\ 1,,.i1 1,.10 1.0 \\ ,{,,.'\\O.e-S~,.'-lS Analyst: ~ ~- Weight per Initial number of larvae (mg) = C / Initial number of larvae >..c.., ~o 4'? ~<-, ~ ,.._'i::> q; A q; I\\. ,...__o ~ \\$) -..Q ...g o* I) Analyst: ~. 0 C) 0 0 () Average weight per initial Percent ~,, number of larvae (mg) reduction Q,lc,i~ \\'S-17. o.fo<! from control tJP.. [%)
Weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520.
Comment codes : c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg= unusually large, d&r = decanted and returned, w = wounded. I Commentsc ~ I 30 of 90 SOP AT20-Revision 6-Exhibit AT20.3
Environmental Testing Sofutlom, Inc. C.tuu:znlr.allll('I 19/t) Coni, ol. Nc.n*trtl'llt>t.l
Conrrol, UV-trr>>lt'd 11.zs*1.
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IH9 10.20 14.17 14.79 16 ll 14 7.: 1-191 159) ls.37 IS.97 i, 47 IHI IHO II 6&
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TV / Sequoyah Nuclear* Plant, Outfall IOI October 06-13, 2020 Pimeplrnles pro1111!1t1s Chronic Whole Ernucut Toxicity Test EPA-821-R-02-0IJ, 'lctho\\l !000.0 Quolity Co111ro.l Verilicatiou of O:,ta Entry, Cnkulations, 11nd Stntislical Analyses Not for CompU."ll~,'U~~rnt-rU, lnn:nul LOl'.atocy QC A s i1<1t11'L,r'l*:1r ~"l,it \\1-tl(!"1 ( Jl,X) Wri<,!~1 1 Sl.lnhl.*t l\\1n1..,tJcfll f C~ffifK'81.,f \\"lirblfl!II Wr\\C'hL f ln"liil t10,IJ1n
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~I 0.769 l'? 9) 7.6 1 0.761 076 1 l l 96 787 0 787 0.787 2 1 95 7.-1 0 741 0.741 21 S6 HS 0 ?JS om ?l 07Jl ll.91 712 071? 071 2 2 1 &6 7.17 0 717 0717 2344 1.65 0.76S 0765 21 16 7 ll 0 751 0.7J'l L) 0.751 l ~ 14 , n on2 0.722 1l.9l 7 57 0.7S7 0757 !O ?l 6J4 06)4 0 bl* 2)07 6.87 0 763 0 692 79 O.~S7 21.S:! 6.70 0 670 0 670 22 IS 701 0701 0701 11n.1rr,un1 1gniftam1 Difl'ercm;c P:l"f'c.en1 M1niulvn1 Signiricaru Dl0-eJcnce .\\lr;tt1*11.r.l* al "1t'Wlu1;1t:h1I C*ffik-111.. 1 f'urcm rcJ1;('1ktfl hum f~<.} l1tltl~ ~o,mb<-r *t , ori.ollM!l l"*-**<el>, ('ntlh'lll f"l,o) h o-.c l~J ,..,i-1 __ _. __, 1-00.0 °'808 6.J
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Low1:r Qnd upptt PM.SD bound;;:,vc-re ():1um,ned from lhr tOlh :md '90111 pm:t:nt1k 1espcc.11vety, cifPMS.O dn:a f,om EPA's WET ln1erl bor.uory V:ansbtluy SU.1dy (USEPA. 2001:1. tJSEPA, 2001h) USE.PA 200111. 1001b fln;i l R,:,pon J111r1laboratory V:m:abd1ry SM1y of E.J'A Shon-le-rm (1\\ron,t Md A(uh! Wbolt £ffiutruT0.1i!1utyT~11 1ethctts. Volcamt! I :.,ti 2.-Appcnd1'.'l. *rA~S21*8-0r-OO-I 4nd EP,\\ *8ll*D*Ol*OOS US E.,,vrronm~11;! r*ro1tc11on Agtncy, C1ncmn;,11, Otl 31 of90
Stan Date: 10/6/2020 T A I Sequoyah uclcar Plant, Outfall 101 October 06-13, 2020 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Test 10: PpFRCR S.imple ID: TVA / SON 101 End Date. 10/1312020 Lab ID ETS-Envlr Testing Sol Sample Type: DMR-Discharge Monitorlng Repon Sample Date: October 2020 Comments: UV-treated Cone-3/4 1 2 UV-Control 0.7750 0.7250 Non-Control 0.8450 0 7900 0.7290 0.7000 0 7690 0 7A50
0. 7510 0.6870 17 25 0.7370 34.5 0 7150 69 0 8100 84.5 0 7410 100 0 7650 Intake 0,6340 Protocol: FWCHR-EPA-B21 -R-02-013 Test Species:
PP-Pimephales promelas 3 4 0.8160 0 8790 0 8530 0 7450 07450 0,8030 0 8220 0.8390 0 7610 0.7870 07120 0 7170 0 7220 0.7570 0.6700 0, 7010 Transform: Untranslormod 1-Talled Isotonic Cone.,% Mean N-Mean Mean Min Max CV% N t-Stat Crltlcal MSD Mean UV-Control 0.7988 0.9882 0 7988 0.7250 0.8790 8.159 4 0.7988 Non-Control 0.8083 1 0000 0 8083 0.7450 0.B530 6.263 4 17 25 0.7535 0,9323 0.7535 0.7290 0.8030 4,465 4 1.455 2.410 0 0750 0 768 1 34,5 0.7690 0.9514 0 7690 0.7000 0.8390 9.313 4 0,957 2.410 0.0750 0.7681 69 0 7818 0.9672 0 7818 0.7610 0.B100 2,782 4 0.547 2 410 0.0750 0.7681 84.5 0 7288 0 9016 0.7288 0,7120 0.7450 2.286 4 2.251 2.410 0.0750 0.7388 100 0 748B 0.92B4 0.7488 0.7220 0,7650 2.502 4 1.608 2 410 0 0750 0 73B8 Intake 06730 0,8327 0 6730 0.6340 0.7010 4,296 4 Auxllla!}'. Tcists Statistic Critical Skow Sl>apiro-Wilk's Test Indicates norrnat dlstributiofl (P > o 01) 0.96745 0.884 0.17067 Bartlett's Test indicates equal v;,ri.inces (P = 0.07) 10.1792 15.0863 Toe con1ro1 means are not slgnificanll~ atlferenl !e = 0.83) 0.23025 2.44691 Hyeothesls Test (1-11111. 0.05! NOEC LOEC ChV TU MSDu MSOe MSB MSE F-Prob Dunnell's Test 100 >1 00 0 07496 b 09384 0.0025 0 00193 0.30966 Treatments,s UV-Control Linear lnterpol:ition (200 Resamplos) Point IC0S IC1 0 IC 15 IC20 IC25 IC40 ICS0 3/4 SD 73,899 >100 >100 >100 >100 >100 >100 09 oe 0 7 .c. i O 5 e C> 0.5 E o~ ,- 0.3 02 95% CL(Exp) Skow 10 -,--------------, 0.9 08 07 5: 0 6 C: g_ 0.5
OA 0.3 0,2

0. 1 00~~~::;:::~=~~~-.-l 0
50 10D 150 Dose-Response Plot Hali, 0.05 level of s111nificance N,Mean 1.0000 0.9616 0,9616 0,9616 0.9249 0.9249 Kurt 0.077 df 5, 18 0 1 0 e

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£nvlronm~nti.llTc1tlo9Solutlon,, Inc Start Date: 10/6/2020 TVA I Sequoyah Nuclear Plant, Outfall 101 - Intake October 06-13, 2020 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Test ID* PpFRCR Sample ID: TVA/ SQN 101, Intake End Date: 10/13/2020 Lab ID: ETS-Envir. Testing Sol Sample Type: DMR-Discharge Monitoring Report Sample Date: October 2020 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Cone-% 1 UV-Control 07750 Non-Control 0.8450 17.25 0.7370 34.5 0.7150 69 0.8100 84.5 0.7410 100 0 7650 Intake 0.6340 2 0.7250 0.7900 0 7290 0.7000 0.7690 0 7450 0.7510 0.6870 3 0 8160 0.8530 0.7450 0.8220 0.7610 0.7120 0.7220 0.6700 4 0.8790 0.7450 0.8030 0 8390 0.7870 0.7170 0 7570 0.7010 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max UV-Control 0.7988 0.9882 0.7988 0,7250 0.8790 Non-Control 0.8083 1.0000 0.8083 0.7450 0.8530 17.25 0.7535 0.9323 0.7535 0.7290 0.8030 34.5 0.7690 0.9514 0,7690 0.7000 0.8390 69 0.7818 0.9672 0.7818 0.7610 0 8100 84.5 0.7288 0.9016 0.7288 0.7120 0.7450 100 0.7488 0.9264 0.7488 0.7220 0 7650
intake 0.6730 0.8327 0.6730 0.6340 0.7010 Auxiliary Tests Shaplro-Wilk's Test indicates normal distribution (p > 0.01 )
F-Test indicates equal variances (p = 0.22) The control means are not significantly different (p = 0.83) Hypothesis Test (1 -tail, 0.05) Homoscedastic t Test indicates significant differences Treatments vs UV-Control CV3/4 8.159 6.263 4.465 9.313 2,782 2.286 2.502 4.298 1-Talled N t-Stat Critlcal MSD 4 4 4 4 4 4 4 4 3.527 1.943 0,0693 Statistic Critical Skew Kurt 0.98396
0. 7 49 0.1363 0.47627 5.076 47.4683 0.23025 2.44691 MSDu MSDp MSB MSE F-Prob df 0.06927 0.08673 0.03163 0.00254 0.01241 1, 6 Dose-Response Plot 0.9 0,8 0.7 s:
j 0.6 e Cl 0.5 ~ C 0.4 0.3 0.2 0.1 0 e e C: 0 <..l 8 (.) C: 0 z 33 of 90 Vl 8
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Page 5 of 7 tnvlronm,mca1Te.nlng Sol\\nk>ns. Inc. Species: Pimephales promelas Date: 10-06-20 Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Daily Chemistry: Temperatures performed at the time of test Initiation, renewal or termination by the analyst Identified In the Daily Renewa l lnrormation table located on Page 1. Alkalinity, hardness and chlorine (total residual) performed by the analyst Identified on the bench sheet specific for each analysis and transcribed to this bench sheet. Day (Analyst identified for each day, performed pH, 0.0. and conductivity measurements only,) 0 l 2 Analyst t--lr TS N'. TI rf'S TS --rs 15 Concentration Parameter pH (S.U.)
6. 18 787 7.99 7.'1~
'i'.ltJ 7.70 Dissolved oxygen (mg/LI 8-o -=1-6' .:q.q 7.9 7.'? 7.3 UV-Treated Con~uctlvity (µmhos/cm) 2"13 310 30g
CONTROL, Alkalinity (mg caco,/l) loO lgQ MHSW Hardness (mg CaCO,/L)
R-0 ~ 11 gq Tempe,ature (*C) '"\\.-.\\,.'\\ '-'-\\,S
ut 1
"\\.,~. I.,, "\\.'-\\.. f "\\.-.\\.\\, pH{S.U.)
6. t"'I 7,~%
l?.00 7.9;;. ~. 10 7&CJ 17.25% Dissolved oxygen (mg/L) fl. o -=1-9 e, 7.9 S?.6 7, :J.. Conductivity {µmhos/cm) <.°19 J;lg<j iaq3 Temperatu*e ('Cl "\\,'":,. O 'U\\. 6 '1.~-1 1-.\\. S "\\.'-\\. '\\ "\\..--l-'\\ pH(S,U.) 6'. 19 i7.27 ~ () I 7.'1 1 Y./1 7Jo9 Dissolvod oxygen (rng/L) 8, I i *"'l 61 ?.Cf 8.6
7. '
34.5% Conductivity (µmhos/cm) 2.'-H ,;JfnQ Pl~~ Temperature j*C) "\\.S. O "\\.'-\\, \\, ')_'{. J '1.'-\\.5 -u-\\. '\\ L-'-\\.\\q pH (S.U.J &,-=,. 7.~ r.oo
7.S?tn
~.II
7. 70 69%
Dissolved oxygen (mg/l) 8 \\ "1*°1 8, I 'i/'.O 8",J 7_ ;:;J_ Conductivity (µmhos/cm) Z.o:3 1-1()~ l.!11.t~ Temperature (0 C) '\\S,. 0 1..'\\.b "-~* e 1..'\\.1 ""\\_<;. 0 '1.-.\\- 6 pH (S.U.) 8./+ 7.~7 i,no 7.S?8 ~.,,
7. 7 ;)
84.5% Dissolved oxygen (mg/l) 6-1 19
6. \\
i.o ¥, J 7.';1 Conductivity {µmhos/cm) (16 /14 Hr'i Temperature ('C) '\\S.. 'I) 7.-\\-~ "'l.*V:\\ '),;~., '\\S, D 1..'\\-5 pH(S.U.) e.,<a
7. 'iJ7 ROI 7_q 1 fr. JO 7.73 Dissolved oxygen (mg/L) g,
-:f."t ~ ~ ft.lJ 'if.O 7..;'.l. Conductivity (µmhos/cm) l'--16 156:J ,.,,.~ 100% Alkalinity 1mg CaCO,/LI lo\\ '5'\\ Hardness (mg CaCO,/L) IDQ l.o 1-Chlorine {mg/l) "-0-\\0 ~ 0.10 Temperature ('C) l..S, O 1.-l-~ lS -0 "\\.~-'1 "1/4, t) 1..'\\.S pH {S.U.)
6. 1'1.
7.~-r;;; 7q<:;
7. (?',

f. /0 7.71..f Dissolved o~vcen (mg/LI 6-1
"::f.q tJ. \\ ~.o 'if, I 7.Co Conductivity (µmhos/cm) 150 /5(p 153 100% Alkalinity (mg CaC01/l) I.,!) sq Intake Hardness (mg CaCOJL) sg lo1-Chlorine (mg/LI ,( 0. l() <.O. tO Temperature ('C) "\\_':,. \\ '\\.~- '5 vv, l..*t~ "\\.S. I '1..'-l-1c Initial Final Initial Final Initial Final I I\\ 34 of 90 SOP AT20-Revision 6-Exhibit AT20.3
Page 6 of 7 Envl,onm*nt.tl Testing Solution 5. Inc. Species: Pimephales prome/as Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 10-06-20 Day (Analyst identified for each day, performed pH, 0.0. and conductivity measurements only.) 3 4 s 6 Analyst 'TS u, LA, IA vL
-s
' l:S rl~ Concentration Parameter pH IS,U.) 8.0;J. 11.uo ~.,..; '.J..~ f>,0-}. 7.'JO ~II 1.'fu Plssoloed ** vern (mc/Ll {s'.O (J.t3 ~-ti II.~ 13.o 7 L.j RO ~-.3 UV-Treated Conduttivlty (µmhoi/cm) 307 ~Oll, ?,01 314
CONTflOL, Alkalinl1v lmc CaCO1/Ll 5°'
.J*'\\' MHSW ' -~ H>rdncss 1mg C*CO,/L) ~I I\\ ,!$1... ~- (\\ T,mpc1ature ('q "'l.'\\. ~ ,A.. ""\\ "\\.V..*\\ "\\..~\\. l,o "'\\..'-\\- ~ "\\..-\\. lo ,.:-1.* 1 "\\...\\.."" pH (5.U.) X'.01-f ~-Si* 8,'7,? '1.'Sol 6.lO 7.94 ?./.3 -q.q 3 17.25% Olssol,ed o,vcen lma/L) f<'. I ~e Ao lo.+ 0,D
7. 3 8'. I
=J.3 Cof1duct111ity (µmho,/cm) oW'I a~ 11.~
lrq T1mp1ro1t1,1rc (vq
'"\\..'-\\.'\\ "\\.'-\\, ~ "'\\..'-\\,6 "'\\..'-\\..e ""l-'-'.,.s '"b,\\.7 "\\.-\\* ~ pf! (5.U.) ll?.o5 i'.\\...;e Q.,5 ~ ;5°1 e.i\\ 7.94 ~/.3 -'1"*4 34.5% Dissolved o,yecn (m1/l) I?: I u."' 0.o Ill* ".l ~.o 7?> r'.. I -::J-i CQnducUvHv l..,imhos/cf'l'll J~I ~.::\\J 1.ru Rwl Temperature (*C) \\.-\\. '\\ .. ~A -..~l-f< "\\. '-\\. '-\\ \\.. '-\\..'\\ "1.'-\\, 5 i.-\\.1 ,_~-~ pH (S.U.) f>. O(p ,;)..58 A.1'-l ~.c:;'l 6.ttl 7,93 8'. 1,J
-=,. '12.
69% D!ssofved oxvg<m tm1/l} 8'. I &.ea 'O l ~R e.,
7. 3
~ o? ":1- 't Conductivity (pmhos/cm) ,14~ ? 1,S a,, A I,] 19' TemptriJtUIC ("C) '1)-\\., "'L"\\, I., "\\..',(J "1.--\\, I.\\ ... --i,." 1.-\\.S "'\\..'-\\. °' "'I.'\\. *..s '+.~ '+,\\A- >.l I 7.91
8. /'71
':l."i'I pH(S.U.) tr.05 0,s Olnolvt:d OJ!.Vl:tn fmi:/l) l?'.I ,n,~ 9.2- 'ln Al 73 ro1 7-. '1 84.5% Conductivity {µmhos/cm} I 'l'll fl?)
~5 11i Tempe.r:atur, (0C)
"\\.~-'1 "\\.-.\\-s 1..'-'- '1 '\\A-'-\\ '-'V'\\ '"1.'-\\-1 "1.'-\\. '1 1.A-1 pH(S.U.) S?.00 'l.llO t'.;.12.. ~,.,,, A.ll ~iC/0 f', IJ ':J,qA orssolved 0Jtv,een (rne/U '6'. I t,!1 H.2 '+.'1 9.2. 7.1-{ i?.3 +.<-f Conduttlvlcy (JJmhoi/~m) ISS l-sn 15; /,t:;.J 100% Alkollr*lty (rnB CaCO,/l) lo\\ Hardness (mg c.aCOJ/L) <:...R Chlorine (ma/L) ~0.1.1."1 Te:'1\\p,r.1tur~ (*q '"tS-o 7.'-\\,5 ,s.o "'1. '-l, I.:. '"\\.S.D 1.. "I
lo l..S.. 0 1~-'1-pH IS.U,)
g".(i~ '.).,l'il.t' "J__e,0 ~;;2, e.\\\\ 7.93 <t.,~ "::J,('j'q 01.uolvtd oMygen (mg/L) ~.l (t:J *"' ~.2- 'l.1 0,L 7"'> 8:3 +.$ Concfu<tl,lty l~mho1/om) 155 14-:\\. L1.H1 151 100% Alkallnlly (mg CaCO,/L) St\\ Intake Hardness lmr CaCO,/L) <..Q Chlodnt (n,£/L) LO.lD T en-,pcrillurc (*C) le;.\\ "1..'-\\-S "\\S...,_ "\\!.l-s LS. e, 7.'-\\.l.:, 'l'-l.'1 11./.l:, Initial Final Initial final Initial Final Initial Final 35 of90 SOP AT20-Revision 6-Exhibit AT20.3
Page 7 of 7 Envlron~nt:alTe~tlng Solutions. Inc. Species: Pimepha/es promelas Client; TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 10-06-20 Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 0 1 z Analyst 1-f 15 f-A) TS r-<J -is. ~ --rs Concentration Parameter pH (S.U.) ~-1'\\ 7.'87 7.99 7. !j/5" io0 7!80 Dissolved oxycen (mg/L) ,.'( =t.9 7.0, ~.O 17 l? 7.7 Non-treated Conductivity (µmhos/cm) ~\\O 3;;_7 ~17
CONTROL, Alkallnity (mg CaCO,/l)
S'1 c:q MHSW Hardness (mg CaCO,/l) ~t.. ~ .¢L) Temperature (*C) 7.4-'\\ '-~l.1., "\\.'\\- i ""'I..'\\, s ""\\..'\\., '"\\.:*\\. s Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 3 4 5 6 Analyst 1.:> IA - vL ~ ~ TS TS ,-1.P Concentration Parameter pH (S.U.) ~.0:2 r:\\..Sl {!), l.D 'lu, e>,,., 7-94 ~.O'a' i-'1'0 Non-treated Dlnolvcd oxyCM jrnQ/ll 7.9 l4.S '-t-8 'l..'-" .1/4-e') 7.:J.. 7.~ 1-. '-I Conove1M1y (f.lmhos/oml 317 ~1'16 1r11.., .1/l'A
CONTROL, Alkalinity !mg CaCO,/L) -
i,..,,..u )\\ SC\\ MHSW c!i;'L . w Hordnus (m& C.CO,/ll Tempera1ure t*c) '\\..4.""'\\ "\\.'\\.S "\\.I..\\.~ \\._'-\\.7 '"'\\.S. 0 1.'-\\.7 "\\:*l. g "\\.5.0 36 of 90 SOP AT20-Revision 6-Exhibit AT20.3
Envlronment.alTecsllng Solutlons, Inc. Conccnrration Parameter lnll (SUI 00 lme/L) Control. Condnctivit-v (lunhosltml Non-rrcn1ed Alknlinity (mg/L CaCO,) 1-J:irdncss (mi/L CaCO,) Tcm r>tr-jl\\lrc ('C) nll (SU) 00 (m~IL)
Control, ConductMtv htmhos/cm)
UV-tre;ited Allrnllniry (mg/L CuCO,J 11:irdncss (mgiL CnCO,) Tcmperolllte (°C) Ill-I (SU) DO(m~IL\\ 17.253/4 Conlluc1ivi1v (umhos/cml Ttmner>1ure ("C) 1111 tSUl DO(mj!/L) H.5% Comlucti\\*itv (11n1hostc111) Tcmocrarure /"C) nll ISU) DO (ml!/1.,I 693/4 Conliuclivil)' (µ mhos/cm> Tcm11er:11ure ("C) 1111-l (Sl.Jl DO (me/L) 8~.5% Conductivi"' h1mhos/cml Tcmocrmurc ("C) lnH /SU) DO(ml!/Ll Co11duc1i,*i1,* lumhos/cm) 100% Alkalinity (mg/L CoCO,) Hardness (mg/L CnCO, )
Totol Residual Clllnrint /m!!/L Tcmncrnturc("C) loll CSU\\
DO (me/L) Contlucli\\*itv lumhos/cm ) 100%, lnla~, AU.nllnily (mi:IL CaCO,) 11Mtlness (m~/L CnC01)
Tomi Rcsltluul Ch lorine (m!!/L Tcnwcrnturc ("C)
TVA/ Sequoyah Nuclear Plant, Outfall 101, UV-treated October 06-13, 20.20 Pimeplwles promell1s C hronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily C hemica l Analyses Day 0 O:wl Dar 2 Dav 3 u.,. 4 In itial Fin:il l11itinl Final lniti:11 final Initial Final Initial
8. 14 7 87 7.99 7.85 8.06 7.80 II.OZ 7.57 8.20 7.8 7.9 7.9 8.0 7.8 7.7 7.9 6.5 7.8 3 10 327 31 7 317 305 57 59 59 86 84 82 24.9 24.6 24.8 24.5 24.7 24.S 24.7 24.5 24,8

8. 18 7.87 7.99 7.94 8.10 7.70 8.02 7.60

8. 15 8.0 7 8 7.9 1:9 7.8 7.3 8.0 6.8 79 293 310 308 307 306 60 60 59 80 84 82 24.9 24.S
'.N.7 '.!4.6 24.8 24,6 24.8 24.1 24,7 819 7 88 8.00 7 'J2 810 169 8.04 7.57 8.22 80 7.9 8 I 7.9 8.D 7,2 S. I 6.8 8.0 279 289 19J 289 279 25 0 24.8 24.7 24.~ 24.9 24.11 24.9 24.3 2,1.1 S. 19 7.87 8.01 7,91
8. 11 7.69 8.0S 7.58

8. 15

8. 1 7.9 8 I 7.9 8.0 71 8.1 6.6 8.0 24 1 260 262 26 1 254 25.0 24.6 24.8 H.5 24.9 24.6 24.9 24.4 24.8

8. 17 7.88 8 00 7.86

8. 11 7.70 8.06 7.58 8 14 8.l 7.9 8.1 8.0

8. 1 72

8. 1 6.Ci

8. 1 203 209 248 2'18 215 15.0 24.6 24.9 20 25.0 24,6 2,1.9 24.6 l4.8 8 17 7.87 8.00 7.88

8. 11 7.72 8.05 7.6<1 S. 15 8 I 7.9 8.1 8.0 8.1 7.2 8.1 6.6 8.2 178 184 184 180 173 25.0 20 24 9 24.7 25.0 24 5 2,19 24.5 24.9

8. 16
-, 87 8.01 7.91 8.!0 7 73 8.06 7.60
8. 12

8. 1 7,9
&.I 8.0 8.0 7.2 BI 6.9 8.2 148 156 155 155 150 61 59 61 60 62 58 <0.10 <0. 10 <0. 10 25.0 24.6 15.0 24.4 25.0 24 5 25:0 24.5 25.0
8. 14 7.85 7,99 7.89

8. 10 7.74 8.06 7.56 7.88

8. 1 7.9 8.1 8.0

8. 1 7.6

8. 1 6.4 82 ISO 156 153 155 147 63 59 59 58 62 58
<0. 10 <0. 10 <0.lO 25.l 24.5 24.9 24.<I
25. 1 24 6

25. l 24,5 25 '.!
Note: Totnl rcs1dunl chlorine was pcrfom1ed on non-1reated Outfall 101 nnd lninke s~mples.
37 of 90 l'inol 7.65 7.<I 24.7 7.59 6.6 24.6 7.59 _ 6.7 24 6 7.59 6.7 24.4 7.59 6.9 24 4 767 70 24 4 7.60 13 24,6 7.53 7 I J*l.5 Project numt,cr: 15420 Day S Dav6 Initial
8. 19 7,8 306 25.0 8.07 s.o 301 24.8

8. 10 8.0 277 24.S 8.11 8.0 254 24 9

8. 10 8 I 216 24.9 8.11 81 175 24.9 8.11 82 153 25.0 8 II 8.2 146 25 0 Final Initial Final 7.94 8.08 7 90 7.2 7.8 74 3 16 24.7 24.8 25.0 7 90 8 II 790 7.4 8.0 73 3 14 2,1.6 20 24.3 7,94

8. 13 7.93 7.3

8. 1 7.3 289 24.5 24.7 2<1.3 7.94 S 13 1.\\1'1 73 8 1 7.4 262 24.S 24.7 10 7 93 8 12 7 92 7.3 8.2 7,4 219
'.!4.5 24 9 2,1.5 7.97
8. 13 7.91 7,3 8.2 7.4 178 24.7 24.9 24.7 7.96

8. 12 7 90 74 8,3 7.4 152 211.6 25.0 24.2 7.93 8.12 7.89 7.3 8.3 7.5 IS i 24.6 24.9 24 6 File: sqn101_ 100620chem-corrected Entered by* T. Sleeper Reviewed by*+-
Page 1 of 7 Chronic Whole Effluent To><icity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA, Sequoyah Nuclear Plant NPDES #: TN 0026450 Project #: _.;:..l S;;_"\\-'-'1.0-=,__ ____ _ Dilution preparation: Dilution prep{%) 17.25 34.S 69 Effluent volume (ml) 345 690 1380 Diluent volume (ml) 1655 1310 620 Total volume (ml) 2000 2000 2000 Test organism source: Organism age: < 24-hours old 84.S 100 1690 2000 310 0 2000 2000 Cate and times lO*Olo*'\\..t> QloOO -TO o'\\oo organisms were born between: Culture board: o"i.* "'\\.."*"\\..o A oa... 'l..""* 1...o e Replicate number: l I l I 3 I 4 s I 6 I 7 I a I 9 I 10 Culture board cup number; ?.'\\ l~\\l I>'\\ I 'fo ~I i I & I '\\ I I~ I I~ Transfer vessel information; pH (S.U.): 1 * '\\'\\. Temperature (*C): '\\S. \\ Average transfer volume (ml): <0.25 ml County: Hamilton Outfall #: 101 Treatment: None Sample was not aerated or treated unless otherwise noted on this form. Sample was warmed to 25.0 +/- 1.o*c in a warm water bath and then diluted to the test concentrations with moderately hard synthetic water (MHSW). Test randomiiotion and location: Randomizing template color: ~ Incubator number 1...&?..- and shelf location: Daily renewal: Day Date Te5t Initiation and feedine, ' Feeding 13atches MHSW Sample numbers used Analyst renewal and feeding, or Selenastrum YWT batch used Outfall 101 Intake termination lime 0 10-0&-20 c:Ai.S o'\\*"'!it)*l..o o'\\*-U* \\.C) o'\\* 1.~
1.o~ 1.0IM,. !\\C:..
111 ICC\\S.()1.:. M 1 10-07-20 D.\\S~ .\\ ~ 1 ~ 2 10-08-20 oQc:.Q d\\*1.\\~1.cL "\\J)IC)\\n, U- ""t.0 H'.)11\\. ~ \\1 3 l0-09-20 (")i S"\\ 1 \\ t J,{ 4 10-10-20 o" o, 10- \\:f\\ *1..oA "'LOI Ocft, \\\\ 1o,ocR.,,._ iR s \\ \\ l \\~ 10-J0-20 b~Sb 6 10-J.~ \\,, -!,r J,, Jt -20 og 1.8 w ~ 7 \\:J .. t-11 10*1 -20 o&"!s,
organisms fel oa~ily 100 L Selenostrum and 100 µL YWT per replicate using HandyStep repeat
µ Chemical analyses: p ipettor SN 17E59354 Parameter Reporting Limit Method number Meter Serial number pH 0.1 s.u. SM 4500-H+ B-2011 Accumet AR20 93312452 Dissolved Oxvgen (D.o., 1.0 rng/l SM 4500-0 G-2011 YSI Model 52CE 180104324 Conductivity 14.9 µmhos/cm SM 2510 B-2011 Accumet AR20 93312452 Alkalinity 5.0 mg CaCO,/L SM 2320 B-2011 Accumet AR20 93312452 Hardness S.O mg CaCOi/l SM 2340 C-2011 Not applicable Not applicable Chlorine, Total Residual 0.1 mg/l ORION 97-70-1977 Accumet AB250 92349123 Temperature o.1 *c SM 25508-2010 Digital Thermometer '>Olo'o'-11..fS Control information: Control-1 ControI*2 Acceptance criteria Summary of test endpoints: % of Male Adults:
07.
D?. .S:203/4 7-day LCso (%} ) 100?, % Adults having 3rd Broods: 1007 100 7
i

603/4 surviving adult5 NOEC (%)

1007,
% Mortality: C)1.
01.

S 203/4 LOEC (%)
> \\00 1. Mean Offspring/Female: 3o. '2, ~o - 'L-
i

15.0 offspring/female ChV (%)
") 1()()7. °lo L'J s.1o l l:,. \\,1.
.,.-~
<'\\t7. IC25 (%) ~I007. t-... \\ 1ndtfHftlil*m ,...,1,wi,,, IC.IOt'tLKltf\\H1" ~ 38 of 90 SOP ATll-Revision 6-Exhibit AT11.2
Page 2 of 7 Species: Ceriodophnia dubia Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 10-06-20 CONTROL-I Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 D 0 0 0 0 0 0 0 0 Adult mortality \\._ \\.._ \\._ L-L L-L. '- 2 You nc produced 0 a 0 0 () 0 0 () 0 a Adult mortality \\.._ L L 3 Yo.ung produced c) 0 0 0 0 0 0 0 0 D Adult mortality L L- \\._. \\.._ L__ L-L \\,_ 4 You ng produced '-\\ ~ '-\\ '-\\ '-\\ '-\\ ~ ~ s s Adult mortality \\.._ L ~ '-- L s Young prod1Jced I\\J l\\ ' \\ '\\ l \\ ' "l_ 'c::i l() I\\ ! ?... Adult mortality \\._ \\_ \\..... \\._ L \\..._... \\,_ 6 Your11: produted () 0 () 0 0 0 0 0 0 0 Adult mortality L.. \\_ L \\.._ \\__ \\...... '- L L 7 Young produced IS, ,~ \\\\o \\ s I'!) \\ \\.o l'"\\ \\ '"!) \\ '-\\ \\~ Total young produced "'l,.."'\\ bo ~\\ l..~ ~o ~'2.. ~'L- '2.8 ~o ~ Final Adult Mortallty I..,..... X for 3rd Broods X. )<.. )".- 7- 'j- ~ ')<-.. )(- '>C_ Note: AduJI mortalltv (L
tr.-e, O is dead}, SB : split brood j5Jr,1rle brood sollt betwtt-n two days}. CO a:. cany 0\\/er (of()prlna: urried over with aduh durJnc tr.1.ndtrt Concentration:
% Mortality: I
07.
Mean Offspring/Female: I '" -~ CONC: 17.25% Survival and Reproduction Data Replicate number Day l 2 3 4 5 6 7 8 9 10 1 Young prod11ced (") 0 0 0 0 0 0 0 n 0 Adult mortality \\.._ L L-L- L l-L- L-L L 2 Young produced n D 0 0 0 ('\\ ri {) 0 0 Adult mortality \\_ \\_ L-L_ L L. L L L L 3 Young produced 0 0 0 D 0 0 0 0 0 0 Adult mortality L L L L \\..- L L L 4 Young produced s '-\\ s '-t b '-\\ L\\ s 'S 'i Adult mortality \\......- \\__ \\.._ \\_. L L L L L \\.._ s Young produced \\ 1.. 10 IL-ID I~ I \\ 11.. IL / () IL.. Adult mortality \\.... \\..__ \\,_ \\.._ L L-L 6 Young produced 0 0 0 0 D 0 0 0 0 0 Adult mortality L L. L L L L-L 7 Young produced 1S \\ '\\ I~ \\\\ \\~ \\CS "~ I 'i 11 11 Total young produced ~7_ :>~
!>S 3-i \\
3)---\\ 3() ~'-\\ .:!) \\ ~1- ~'.:) Fini!I Adult Mortality L L L. '- I..__ L... L L L. Not~* Adult rnonamv (L = 1lvt>, D:. deid), SB== 1pllt brood {Jingle brood spf,l bet we.en lWO days), CO ~ c-11ry over (offspnnr carried over whh idult durln,c transft!r). Concentration: % Mortality: ()7, Mean Offspring/Female: -.:,2.. 5 % Reduction from Control-1: '~7. 39 of90 SOP AT11-Revision 6-Exhibit ATll.2
Page 3 of 7 Species: Ceriodaphnia dubia Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 10-06-20 CONC: 34.5% Survival and Reproduction Data Replicate number Oay 1 2 3 4 5 6 7 8 9 10 l Young produced 0 0 0 0 C) 0 0 0 0 0 Adult mortality \\.._. '- \\_ '- L 2 Young produced 0 0 0 0 0 C) D n C) () Adult mortality \\_ '--- L L L \\.._ \\,_ 3 Young produced Ii (\\ C) 0 0 0 0 0 0 0 Adult mortality \\.._ L.. 'l__ L L__ L L 4 Young produced s 4 s '-\\ '--\\ ~ ~ ~ ~ s Adult mortality \\.._ \\,_ L. 5 Young produced \\ 1- \\ 'C) \\ 1... .'\\ ,1.... \\~ 10 '~,,_ 10 Adult mortality \\._. L \\._ I..._ 6 Young produced D D 0 ri 0 0 0 0 0 (1 Ad1-1lt mortality L \\_ \\_ \\._ L L.... L 7 Yo1-1ng produced \\'""\\ \\ "\\ \\\\ \\ lo ,'\\ \\ C\\ I'S I\\. \\~ ,g Total young produced ~--\\ ~::>
>'-\\
~";)
>'5
~\\ ~I.:)
>1,.
~1... ~~ Fin.ii Adult Mortality Not~* Adult mo,tiltty (L
llv.e, O s dud), sa ~ spilt brood (sfr,alr brood ipllt betwel!n two davJ:t. CO

c;1r,v over fofbprlne carried over with ~duh durlr,,IIJ transfer)
Concentration: % Mortallty: D7. Mean Offspring/Female:
'.)";.~
% Reduction from Control-1: -"\\.'\\ 1. CONC: 69% Survival and Reproduction Data Replicate riumber Day 1 2 3 4 s 6 7 8 9 10 1 Young produced n () () () n 0 () 0 0 () Adult mortality L L.. L-L- L L L L.. L L 2 Young produced 0 0 0 D ('I a ('\\ () 0 0 Adult mortality L L \\._.. \\._.. L L L L L 3 Young produced 0 0 0 0 0 0 (\\ C) 0 0 Adult mortality L \\._ \\._ \\._.. \\.._ '-- \\._ L-L. L 4 Young produced ~ \\o ---\\ l.o '4 to "S s lo Adult mortality L \\_ \\........ \\._ L. L L \\._ \\_ '- 5 Young produ~ed \\ 1- \\:::, \\ \\ JO 11._,,_ l"L \\1-I\\ Adult mortality L.. \\.... L.. \\.... \\,_ 6 Young produced n 0 0 6 0 0 0 0 0 0 Adult mortality L L L L.. L L L L L-7 Young produced l~ \\ '\\ l "\\ 1.1:l \\ °' \\ \\.o \\" \\ <.. 1""\\ 1..:D Total young produced ~l..
)~
~'--\\ !>'\\ °!:,'5 3'1..
n
~~
!:.'-t ~,
Final Adult Mortality L \\._ \\..._ \\._ \\.._.. Nore: Adu!\\ mortalily (l
live, 0 ~ dud), SB

split brood (sln&l6 brood spJit be ween two days}, CO e earry ovu (0ff,pring tarried overwltl't adult du,*io1 tf".)nifer).
Concentration: % Mortality:
01.
Mean Offspring/Female: 3S.I 1/4 Reduction from Controi-1: - IS. 8 7. 40 of90 SOP AT11-Hevision 6-Exhibit ATll.2
Page 4 of 7 Species: Ceriodophnia dubia Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date: 10-06-20 CONC: 84.5% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 B 9 10 1 Young produced 0 0 0 0 0 0 0 0 D 0 Adult mortality L. L. '- L.. \\__. \\.._ L L.. L L 2 Young produced n 0 0 0 C) a 0 0 0 0 Adult mortality L-L- \\.._ \\_ L.. 3 Y,oung produced 0 0 0 0 0 0 0 0 () 0 Adult mortality L.. L. '- \\__ \\_ L L 4 Young produced \\o 'S le I.., '-\\ 'S s ~ \\ .s Adult mortality \\..... \\_ \\...... 5 Young prod1,1ced \\~ l~ ,1-IC \\:::, \\1.,. \\. 't) \\ \\ \\1- \\1-. Adult mortality \\._ '- \\_ '- I._ 6 Young produced 0 n 0 0 n 0 0 a 0 0 Adult mortillity L-L. \\_ L-L_ L L L L 7 Young produced u \\R I '"\\ ,~ 18 \\~ \\-<\\ '~ '1...C \\& Total young produced '-!o ~I. ~s ~s ~s ~\\. ~'\\ ~'-\\ ~~ ~s flnal Adult Mortality \\.... Not~: Adult mono1h1f (l: """* D :e de.d), SS = 1pJit brood lsln,1le brood,pfll bttwel!n 1wo d1yJ). CO 11 carry ove.r(ofhprlnc c:iarried ov'°r with.adult duri111 tr.msfer) Concentration: % Mortality: 07, Mean Offspring/Female: ~-" % Reduction from Control-1: -,8,S 1. CONC: 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 l Young produced C) 6 0 0 0 0 0 () 0 0 Adult mortality L-L l_... L L-L- L l...- L \\ 2 Young produced 0 0 l\\ 0 {') (\\ C) 0 0 0 Adult mortality L L L L_ L._ L._ L L 3 Young produced 0 0 D D () () () a 0 0 Adult mortality L \\._ \\._ \\_ L. \\_ \\._ L \\._ 4 Yo1.1ng produced "'"\\
'S lo
~
s.
L, s l.o '5 s Adult mortality L \\.._ L L \\,._ L \\_ L 5 Yourig produced i~ \\ '-\\ I "'L... \\ "L 1'-\\ I\\ I'\\ \\L I~ Ad1.1lt mortality \\._ '- \\___ '\\.._ '-- \\._ \\._ 6 Yo1.1ng produced 0 0 0 0 0 0 0 0 0 0 Adult mortality \\._ L.. \\.._ \\.._ L L 7 Young produced \\ 1-C l°' \\ 1- \\'\\
1. \\
\\ ~ \\ '\\ \\ '\\ l.D Total young produce d 3°1
>°'
~7 ~s ~I.:, "-\\ \\ ~'-\\ ",)'i ~b ~s-Final Adult Mortality L- \\,_ '- L \\._ L \\_ L Notf* Aduh mort1Hty (L.: lln, O 11:: dead), ~B:;: ~pU1 brood {slnale brood split between tw~ davsl, CO
carry ovtr (off.spring tirried over with.adult d11rln1 tr1ruferl, Concentration:
% Mortality:
01.
Mean Offspring/Female: Jl,-.\\ % Reduction from Control-1: ~1.~-~ 1. 41 of 90 SOP ATll-Revision 6-Exhibit ATll.2
(..,..,.antot111, l...,ll"'9s.hlt>>f!LIIIC. Page 5 of 7 Species: Ceriodaphnia dubia Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Date : !Q.:Q&.:.?.Q CONTROL-2 Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced a ~ a a D 0 0 0 a 0 Adult mortality L \\._ \\._ \\.....- L. L.- L- \\,_ 2 Young produced 0 a C) 0 0 0 0 C) C) L) Adult mortality L L \\..._ 3 Young produced a a (') 0 0 0 0 0 0 0 Adult mortality L-L- '- L \\,_.. 4 Young produced ~ "-\\ "S '-\\ 'S ~ '-\\ '{ '--l Ad ult mortality \\_. \\.__ \\.._.. \\..- s Young produced l l ,c /Q '\\ I "L, lb \\b \\0 ID \\~ Adult mortality L \\_. \\....- \\..,.... L 6 Young produced D Q 0 0 D 0 a 0 0 6 Adult mortality L \\.._ \\,__ L. I.,__... 7 Young produced I'-\\ I':.:> '~,, \\ I,,, \\ "'"\\ \\ '-\\ \\ <:::. \\~ \\S Total young produced !>b ,..1.. -,_c:; ~\\ ~,_
, 1..

.'"l..
"\\... '\\ ?..'\\
,'L.
Final Adult Mortality \\._ ~ \\,_ \\._... \\......- X for 3** Broods )(_ y.... ';;L 7'-- -y_ -y.. )<... ~ ~ -y:... Nolt!' Adult rnornJUy {! = hve, 0 ~ dnd), SB c ss,Ut brood (smclc broad s.plh belwe~fl two d;,vst, CO* c,,ury.oyer (cff, pun1 curled over-with ilduh during tr.ir,,s,ficr). Concentration: 3/4 Mortality: I () 1. Mean Offspring/Female: I..30.1.... coNc: 100% Intake Survival and Reproduction Data Replicate number Oay 1 2 3 4 5 6 7 8 9 10 1 Young produced () C'i D 0 0 0 0 0 0 n Adult mortality \\.._ L L L. L. L "L L... L 2 Young produced t\\ a 0 0 0 0 (') C) () 0 Adult mortality L L \\._ L. L L \\._ \\_ l_ 3 Young produced 0 0 0 0 0 0 u 0 0 0 Adult mortality L L L... \\.._ \\.._ \\_ '- L L 4 Young produced \\:, ~ ~ c:;. '"\\ '5 ~ \\ \\o ~ Adult mortality L L L L \\,_ \\,___ L 5 You11g produced I'--\\ \\ l.- , 1 \\ I.... \\Q I '"L I~ \\.'::::, II I '1.... Adult mortality \\_ \\._ L \\.._ L l-L \\._ 6 Young produced ('\\ 0 0 0 C 0 0 0 0 (") Adult mortality L-L \\.. L L L L \\,_ 7 Young produced I 'S, ,g ,s 1q IL\\ 'e l ') *~ I~ ,1 Total young produced ?>S ~I. !>1.. 31,.:,
>lo
~s ~'-- ~s ~$ 3~ Final Adult Mortality L L L... No!t; Adult moruhtv (l : l1\\/e, 0., de1d}, SB s split brood (slngle brood 5p\\1t between two dayi), CO= catrv over (ofhprlr1r c*rried over with adult dut1ne tr.1n.d~r). Concentration: % Mortality:
01.
Mean Offspring/Female:
,'\\. '\\
% Reduction from Control-2: -,S.b7, 42 of 90 SOP ATll-Revision 6-Exhibit ATll.2
E~'m'CJ nfflen1-1ITt.1Uo9 SofLH ktnt, Inc Control-I 0:1y Reolic:.re-.number 2 3 .j 5 6 7 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 l 0 0 0 0 0 0 0 .j
s *

4 4
5 s 10 I I 11 9 I I 12 10 6 0 0 0 0 0 0 0 7 15 14 16 15 IS 16 17 Taul 29 30 JI 28 30 l? 32 17.25% Doy Reollc:t.tt' number I 2 3 4 s 6 7 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 J 0 0 0 0 0 0 0 .j s ,1 .) 6 -I .j s 12 IO I~ 10 IJ I I 12 6 0 0 0 0 0 0 0 7 15 19 18 17 15 15 18 Tomi 32 33 35 3 1 34 J O 3* 34.5'!ro Day flcolic t1 tt' number I 2 3 4 5 6 7 I 0 0 0 0 0 0 0 l 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 4 5 5 4 4 5 5 5 12 10 13 l l 12 ll 10 6 0 0 0 0 0 0 0 7 11 19 17 16 19 19 15 T ot.a l 34 33 3,. 33 JS 37 JO 69% Doy Rcnl.icale-numbtr I l J 4 5 6 7 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 s b 4 6 6 4 6 s 11 l l I I 13 10 12 6 0 0 0 0 0 0 0 7 IS 19 I? 20 19 lb 19 T ot.1 1 32 38 3~ )9 35 32 37 43 of 90 TVA/ Scq uo)*ah Nuclear Plant, Outfall 101 October 06-13, 2020 Verification of Ceriodaf)l111ia Reprod uction Totals 84.5% 8 9 10 Tot::i1 D*y I 2 3 0 0 0 0 l 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 J 0 0 0 s 5 5
s 6
j 6 10 11 12 107 s IJ IJ 11 0 0 0 0 6 0 0 0 IJ 14 16 151 7 2 1 I S 17 28 JO 33, 303 Totol .. o 36 35 100% 8 9 10 To1a l DD)' I 2 3 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 0 5 5 4 .J6 7 5 6 12 10 12 114 5 13 14 0 0 0 D 6 0 0 0 14 17 17 165 7 19 20 l'l 31 32 33 32S Total 39 39 37 Conlrol-2 8 9 JU Tocal 'Dny 1 2 J 0 0 0 0 J 0 0 0 0 0 0 0 1 0 0 0 a 0 0 0 3 0 0 0 5 s 5 47 5 J ~ 10 12 10 114 s 11 10 10 0 0 0 0 6 0 0 0 17 15 18 172 7 14 13 l5 32 32 33 333 To10I JO 26 29 JOO3/4 Intake 8 9 10 Tornl Day I 2 3 0 0 0 0 I 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 J 0 0 0 5 s 6 SJ ~ 6 6 6 n 12 II 118 s 14 12 11 0 0 0 0 G 0 0 0 16 17 20 180 7 15 18 15 33 3* 37 351 To.1.al 35 36 32 Rcnlic:11c-numb~r Tot:.I ,I s 6 7 s 9 10 0 u 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 u 0 0 0 6 4 5 5 5 7 s 5,1 10 JJ 11 10 11 12 12 II S 0 0 0 0 () 0 0 0 19 I R 19 19 18 ?Q 18 187 J S JS 36 J,1 J,1 39 35 359 Re,lic:ue nurnbc-r Toral 4 5 6 7 6 9 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 a 0 6 s 6 s 6 5 5 56 12 12 I,I II 1.t 12 IJ 127 0 0 0 0 0 0 0 0 17 19 21 18 19 19 20 191 JS JG 41 J,1 39 36 38 374 lhl')litatc number Tomi -I 5 6 7 8 9 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s 4 s s 4 .t 4 ] q l2 10 IJ 10 10 IJ tOS 0 0 0 0 0 0 0 0 17 16 17 1q 15 15 15 151 _j j 32 3l 31 19 I 29 32 302 Re tlic.:,tt nu.mb~r Tor:11 s 6 7 s 9 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 7 5 5 7 6 4 51 n 10 12 12 IJ I I 12 119 0 0 0 0 0 0 0 0 19 19 IS 19 IS 13 17 173 36 36 35 36 JS JS JJ 3-l!J ~-:---r
Environmental Testing Solutions, Inc. ConccnlTntion (%) I 2 J Con1rol - I 29 30 31 17.25% 32 33 35 34.5% 34 33 34 69% 32 38 34 84.5% 40 36 35 100% 39 39 37 Control-2 30 26 29 100% Intake 35 36 32 Outfoll 101: DunncU's MSD value: 2.050 PMSD: 6.8 In lake: Dunnelt's MSO value: 1.324 PMSD: 4.4 TVA/ Sequoyah Nuclear Plant, Outfall 101 October 06-13, 2020 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Ceriodaplmin dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical nalyscs 4 28 31 33 39 35 35 31 36 MSD= PMSD = Projccl number: ___________ 1_5_42_0_ Rcplic:Hc number S urvival Average reprod uction Co<'fficient of Pf:rccnt rctluccion from (%) (oITspring/fcmnle) ,*nri.ation (o/* ) pooled ton1rols {"'/.,) 5 6 7 8 9 10 30 32 32 28 30
3) 100 30.)
S.6 Nol npplicnble 34 30 34 31 32 33 100 32.5 <l.9 -7.3 35 37 30 32 32 33 100 33.3 5.7 -9.9 35 )2 37 33 34 37 100 35,1 7.2
1S.8 3.5 36 34 34 39 35 100 35.9 5.6
-18.5 36 41 34 39 36 JS JOO 37.4 5.8 -23.4 32 2 32 29 29 32 100 30.2 6.6 Not npplieable 36 35 36 35 35 33 100 J:1.9 l.9 -15.6 Minimum Significant Difference Percenl Mintmum SignHica111 Differe11cc PMSD is a measure oftes1 precision. The PMSD i~ lhc minilJlum pcrcen1 difference between lhc control and 1rcalme1111ha1 can be declared Stalistically significant in n whole effiuent toKicity test Lower PMSD bound detcm1ined by USEPA ( 10th percemi.le) "' I}%. Upper PMSD bound dc1em1ined by USEP!\\ (901~ percentile)= 47%. Lower and upper PMSD bounds were de1cnni11ctl from the 10th and 90lh percentile. respeclivcly. of PMSD data from EPA's WET ln1crlabora1ory Variability Study (USEPA, 2001J: USEPA. 2001 b) USEPA. 200 la. 2001 b. Final Rcpor1* lntcrlaborat0ry Vanability Smdy ol' EPA Short-term Chronic and Acule Whole Effi11cnl Toxicity Tesl Methods, Volumes I and 2-Appcndi.~. EPA-821-13-01-004 and EPA-821-8-01-005. US Environmental Protection Agency. Cincinnati, OH. 44 of 90
E.""vlro"'""'"*' l1>Jdr,g 5*Nlloru. tnt. Slart Date 101612020 TVA/ Sequoyah uclear Plant, Outfall 101 October 06-13, 2020 Statisrical Analyses Corlodaehnia Survival and Roeroduclion Tost-.Roeroduction Test ID* CdFRCR Sample ID* TVAISQN101 End Dato. 10113/2020 Lab ID. ETS-Envir. Testing Sol. Sample fype* DMR-Olscnarge Monllonng Report Sample Date* October 202 0 Protocol FWCHR-EPA-821-R-02-013 Test Species: CD-Cerlodaphn1a dubia Comments Conc*o/* 1 2 3 4 6 6 7 8 Control-1 29000 30.000 31 000 28.000 30 000 32.000 32 000 28.000 Control-2 30.000 26.000 29 000 31 000 32.000 32 000 32 000 29.000 17.25 32 000 33.000 35.000 31 000 34 000 30 000 34.000 31 000 34.5 34.000 33.000 34 000 33.000 35.000 37 000 30.000 32.000 69 32.000 38.000 34 000 39.000 35.000 32 000 3i.000 33 000 84.5 40.000 36 000 35.000 35 000 35.000 36.000 34 ODO 34 000 100 39.000 39.000 37 000 35 000 36.000 41.000 3~ 000 39 000 lr,lake 35 000 36 000 32 000 36 000 36 000 35 000 36.000 35.000 Transform: Untransformod Con0-*1o Moan N-Moan Moan Min Max CV0/. N t*Stat Conlrol-1 30.300 1.0033 30.300 28.000 33.000 5 620 10 Canlrol-2 30 200 1 0000 30.200 26 000 32.000 6.566 10 17 25 3:2 500 1.0762 32 500 30.000 35 000 A 865 10 -2.455 34.5 33_300 1.1026 33 300 30.000 37.000 5.671 10 -3 347 69
35. 100 1.1623 35 100 32 000 39 000 7164 10
5.355 84 5 SS.900 1-1887 35.900 34 000 40 000 5640 10
-6 248 100 37 400 1.2384 37.400 34 000 41 000 5 804 10 .7 !'122 Intake 34.!l00 1 1556 34,900 32000 36 000 3 926 10 Auxlll*~ Tests St~tlstle Kolmogorov D Test indicate~ normal distrlbulion (p > 0 0 1) 0 88003 Bar!lell'* Tesl md,ca1es equal v;:,rianccs (p = 0.78) 2 45982 The control means are 1101 slgnificantl~ dlfferont (e ; O 91 l 0.1 2078 H~!!olhosls Tosi j1-tail, o.osi NOEC LOEC ChV TU MSPu MSDe Ouonell's TMl 100 >100 2.04951 0.06764 frea1men1s vs Con1ro1.1 Lin oar lntorpo!atJim {200 Roumplos) Point !COS IClO IC15 IC20 1C25 IC40 IC50 3/4 >100 >100 >100 >100 >100 >100 >100 SD 95'/, CL S~ow ~ 0 ~ O! 1 0 09 08 0 7 06 OS 04 0.3 02
0. 1 0.0
.o 1 .Q,2 -0 3 0 9 10 30000 33.000 29.000 32 000 32 000 33000 32 000 33.000 34 000 37 000 39000 35.000 36 000 38 D00 35 000 33000 1-Tailod Critical MSO 2.287 2.050 2 287 2 050 2.287 2 050 2 287 2.050 2.287 2.050 Critical 1 035 15.0663 2.10092 MSEf. MSE 65.5367 4 01687 50 100 Doso o/.. 45 of 90 Ociso-Rospor,sv Plot 45,---------------------, 40 35 " 30 0-- --- 0 "' g 25 'O e 20 e-rr 15 10 a i N ~ ~ ~ u u ~
i:
~ ~ i i ~ Hail, 0.05 level of significance Isotonic Moan N*Moan 34.083 1 0000 34 0B3 1 0000 34.063 1 0000 3~ 083 1 0000 34 083 1 0000 34 083 1 0000 Skow Kurt 0.3173 -0 5913 F-Prob df 8.8E-10 5, 54 150
TVA I Sequoyah Nudear Plant, OutfaJJ 101 - Intake October 06-13, 2020 Statistical Analyses Envlronme.nt:111 Tes-Ung Solutions. Inc Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 10/6/2020 Test 10* CdFRCR Sample ID: TVA/ SQN101. Intake End Date: 10/13/2020 Lab ID: ETS-Envir. Testing Sot. Sample Type: DMR-Discharge Monitoring Report Sample Date: October 2020 Protocol: FWCHR-EPA-621-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Cone-% 1 2 3 4 6 6 Controt-1 29.000 30.000 31.000 28.000 30.000 32.000 Control-2 30.000 26.000 29.000 31 000 32.000 32.000 17.25 32.000 33.000 35 000 31,000 34.000 30.000 34.5 34.000 33,000 34000 33 000 35.000 37.000 69 32.000 38.000 34.000 39.000 35.000 32.000 64.5 40.000 36.000 35.000 35.000 35,000 36.000 100 39.000 39.000 37,000 35.000 36.000 41.000 Intake 35.000 36.000 32.000 36.000 36 000 35.000 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max Control-1 30.300 1,0033 30.300 2B.000 33.000 Control-2 30.200 1.0000 30.200 26.000 32.000 17,25 32,500 1.0762 32.500 30.000 35 000 34.5 33.300 1.1026 33.300 30.000 37.000 69 35.100 1 1623 35,100 32.000 39.000 84 S 35.900 1.1 B87 35900 34.000 40.000 100 37.400 1.2384 37.400 34.000 41.000 Intake 34,900 1.1556 34.900 32.000 36.000 Au)(i11;1ry Tests Shaplro-Wilk's Test Indicates normal distribution (p > 0.01) F-Test Indicates equal variances (p = 0.28) The control means are not significantly different (p = 0.91) Hypothesis Test (1-tail, 0.05) Homoscedasllc I Test indicates no significant differences Treatments vs Controt-2 CV3/4 5.620 6,586 4.865 5.671 7.164 5.640 5.604 3.926 7 32 000 32.000 34.000 30.000 37.000 34.000 34.000 36,000 N 10 10 10 10 10 10 10 10 Statistic 0 89404 2 10651 0.12078 MSDu 1.32441 Dose-Response Plot 45 40 35 C 30 0 ti 25 "C e 20
a.
QI a: 15 10 5 0 ~ Ill Ill e N ~ ~ c 0 u u 46 of 90 8 9 10 28.000 30.000 33.000 29.000 29.000 32.000 31 000 32.000 33.000 32 000 32.000 33.000 33.000 34.000 37,000 34.000 39,000 35.000 39.000 36.000 38.000 35.000 35,000 33.000 1-Tailed t-Stat Critical MSD -6.154 1.734 1,324 Critical Skew Kurt 0.866 -1 0359 0.7129 6.54109 2.10092 MSOp MSB MSE F-Prob df 0.04385 110.45 2.91667 B.2E-06 1, 18 1-tail, 0.05 level of significance ....... ti.,. i-
\\--- ffl<,t,_,..u.u, k:1Ur19Sowtlo*n. inc. Page 6 of 7 Species: Ceriodophnia dubio Date: 10-06-20 Client: TVA/ Sequoyah Nuclear Plant, Outfall 101 Daily Chemistry: Temperatures performed at the time of test inihation, renewal or termination by the ana lyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and chlorine (total residual) performed by the analyst ide ntified on the bench sheet specific for each analysis and transcribed to this bench sheet. Analy5t Concentration Parameter pH (S.U.) ,..,,J 10i'.U'7,3 Dissolved oxygen jmg/L)
CONTROL, Cond~ctivity (µmhos/cm)
MHSW Alkalinity (mg CaCOJ/l) Hardness {mg CaC01/l) Temperature ('CJ pH (S.U.) Dissolved oxygen (mg/L) 17.2S% Conductivity (µmhos/crnJ Temperature (*C) pH (5.U.) 34.S% Dissolv*d oxygen (mg/L) Conductivity (µmhos/cm) Temperature ('C) pH (5,U.) Dissolved oxygen (mg/l) 69% Condu<tMty (µmhos/cm) Temperature ('CJ pH (S.U.) 84.5% Dissolved oxygen (mg/L) Conductlvlly (µrnhos/crnl Temperature ('C) pH (S.U.) Dissolved o*ygen (rng/l) Conductivity (µmhos/cm) 100% Alkalinity (mg CaCOJ l) 1-!ardnes.s (mg CaCO,/L! Chlorine (mg/l) Temperature ('CJ pH (S.U.) Dissolved o*ygen (mg/L) Conductivity (µmhos/cm) 100% Alkalinity (mg CaCOJ/ll lr,take Hardness (mg CaCO,/l) Chlorine (mg/L) Temperature ('C) 47 of 90 Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) Hr 1-Z G'. 1'-I 1-~ "3 lo S'1 i<.. 1...-\\. "I fl,'2S 8.a 21"!3 '1.<;.o 8.'Z.3 6?-o 2.S"i '-s.o t/,?n R..n /'Ft \\.<;. 0 {i', r=f B.o Pi~ ""\\S. 0 6.tb /t..r-, /'-t"f l.o \\ 58 < 0,1-0 '15,-0 1:--/(,, fl-a /$~- l.,, \\ c:;.p <o.1u '\\.S.O Initial 0 1 T'S Ml --rs,,,r;- T'S '/.OC C-'l ,s. \\ r.oo {3.? '\\.\\\\-'\\ 7.19 6.1 1..'-'\\." 7/19 (!...? 7...'-\\.'\\
7. 9CJ e.~
""\\S *.,_ 7.99 8.~ "\\.4,. ~ 79'1 e,3 "'l~.O final 799 ~.O'"I "::fq ~o 3:n .. ~-t.o ~..

"1.~ *.J "15. 0 l..::r.~ ..;,._\\..vec;. ~ 2 ,:3 TC:, S?,O(p 7.q,g 7 r;- 7.9 317 $'{ 6'~ '"'--\\.* t ,c:_1 it~ 7.9'7 7.9 7_9 ~'13 '-'1-~ lS.D 1i13 ~.00 Sr.O 7..9 ~ln"f '1.<\\."'\\ ls. t)

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'?.O~ g:o 79 ~, '1.'\\.'\\ 1.'-\\-~ ?.O'l' <?.o.;;2 ~-' 7.9 ,ro '"IS -D 1:\\-'\\ r.or ~.03 ~- I 7.9 }5Y \\o\\ I to'1 CQ,10 "\\£. -0 l.'\\-~ '6', /I li?.O 3 ~- ~ ':;-,0 155 .... ~ b, ~0-,0 'lS.o 1s.o Initial Final SOP ATll-Revision 6-Exhibit AT11.2 Page 7 of 7 Species: Ceriodophnia dubia Client: TVA/ Sequoyah Nuclear Plant. Outfall 101 Date: 10-06-20 Dav (Analyst identified for each day, performed pH, 0.0. and conductivity measurements only.) 3 4 s 6 An.ilyst TS vL-vt.-- IA IA- -,-5 -rs rlJ' Concentration Parameter pH (S.U,I 'lo:2 0.10 '3.2,t) Cb.Ov 0.lq '?.07 ~-0~ '8-o-=f Olssoh,rd o*nen (m&/l) 7.9 -1,.e, '+-~ '+;1 'lA 7,'1 7~ (;..Q

CONTROL, Condueth1lty (µ rJthos/cmt

.117 JO'S 30b 3/fo MHSW Alkallnlly (m1 CaC01/L) ,l* 'UI sq Hardneu (m1 CaCO,/L) ~ .. 1-1 &1.- ~ 11v f,.mp~iUUrt rcJ -..--1.~ i..(,. "'L ""\\.'\\. &- "'\\.'-\\. '\\ "'\\.-.\\.& "'\\S. \\ "1'-l-<£ 7..<..0 pH (S.U.) 8'.07 tl>.\\2. '2>.l':, e>.J:\\ 0.1~ S? 1)5?° 8./~ 8-.o--t 17.25% OJnolvc:d oxygC!n (mt/I.) s.o ')-.~ >.O

~.e

~~ 7.<J 79 -=l-"f. CondutlMty {1,1mho1/cm) "193 a"'l-3 ~+~ J..27 Ttmpt:r\\llurr i*c) \\.S.. 0 "\\.'-\\. '\\ "'\\..'-\\. ~ ~<;.. '"\\.., "'\\.--\\. I'\\ 7.-t-8 ""\\.'{. ~ '\\$. 'L pH [S.U.l 8'.08 Ml. e>.2."Z-G.lf+ ~-l1 8'.08' 1?.15 ~3. 34.5% Oluolved o*ygen [m1/l) S'. 0 '\\-A e.o "..\\.E3 't'l

7. '1 7.9

/j..o Conductivity (µmhos/e.m) Jr,7 ~2. "~ .263 Te:mptr-atutt (-Cl "\\S- 0 '\\.'-\\.'\\ \\.'\\..f '\\.--\\. s- "\\.'-\\.'\\ ""\\..>\\. G l.L{, 'i \\.<; -~ pH(~.U.J fol? '0,\\4 0.'l.l e~ l3.l5 ~.07 ~IS 6'-o\\ Otnolved 0111:vi::en (mc/lJ fl,() .\\-~ ri\\i')t,,0 'tl3 A.o ?-.o 7.'1 tJ..o 69% J(J'l '-'-'s ~'i'6' Condu<tl*IIY (~mho,/cm) ~,o, ~ Temperature 1°C) 1$. Q ,4A "\\.'\\. %' "\\..~-'\\ "\\.'-\\.°' \\.'i* ~ 1.'-!., "\\.<;."'\\_ pHIS,U.I f/.09 8.\\-c; e>-2'2., 0,\\0 ~~tu 8.08' '?. /3 &'-ol Olnolved **vcen (m'11-l <i?,O B.o 0.o . t.'} e,.o %.O 7.(j 81 84.5% lf.3 ne, 190 ConducU*lly (µmhos/cm) 1--:\\~ Temper.;,wr, (cc) '\\.S. {) \\_<;. \\ °'\\..{."\\ '\\.'\\.c::. i..'-\\.'\\ L"l-; 1.5. () ""\\£. 'L. p~(S.U.) toCJ <o.,"' 6,,@, ~ -~ e.,, '/.09

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011101,.d **vacn (mil/LI

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(!Jo e.1 8".0 75 e.1 Coodut1Jvlty (µmho,/cml 157 MC:: l'5\\{ 15:J.. 100% Alkallnlty (mi; C.CO,/LI lt, \\ Hu.dneu: {mr c,co,/LJ SJ' Chlotln* in,c/l) <- 0. ro Tt!mpcnturc ('C) U--0 '--..\\.. r \\,.<,.o '\\<;.o "\\.<;. 0 "\\.S, D 1s.o 7..S-'-\\ pH (S.U,) fl.O(o 0,l ~ 6J~ 8,o6 e.w 5?. Olf i.11 6,co Obtolvtd OJCytcn lm.c/lt 8'. 0 ().\\ (j,C 0.t 9.f '(?. I ~o ~\\ Conductivity (µmhos/<ml 157 IL\\' l'-l'=\\ /S4 100% 5'\\ Intake Alk,llnlJy Ima c,COJLI Hardneu lm1 c,co,/LI loO Chlo,inc (ms/l) zo.,o f emptri.ltUrt {0C) N. L~. t> 1.S- \\ '1,_<;.Q "l.'--\\..g 15 *1.. 1~., '1.$. \\ Initial Final Initial Final Initial Final Initial Final SOP ATll-Revision 6-Exhibit ATl 1.2 48 of 90 \\ Environmental Testing Solutions, Inc. Conccntr:nion P;.1ramctcr loll /SU) DO rmi,JL) Conducth*ity (1,1mhos/cm) Control Alklllinity (mg/L Ci,CO3) Hardness (mg/L CaCO,) Tcmnerature (°C) lnll (SU) 17.zs*;., DO rm!!IL\\ Conlluctivitv (µmhos/cm\\ . Ttmoeraturc ("C) lrlll (SU) DO(m!!/L) 34.5% ConducriVitv (umhos/cm) Temperature ("C) oil (SU) OO!m!!/L) 69% Conductivitv (umhos/cm l Tempcramre r"C) oH ISUl DO/mi,JU 84.5% Conduc1ivi1,* (µmhos/cml Tcmpeniture (' Cl nH (SU) OOrmt>/ U Conductivity (1,1mhos/cm) 100% Alk;tlinity (mg/L CaCO>) H~rdness (mg/L CllCO,) Total Residual Cltlorinc lm!!/Ll Tcmpcramrc ("C) lllH <SU\\ DO(me/Ll Conductivity (µmhos/cm) 1003/4 lntnke Alk;tlinity (mg/L CaCO,) llarclness (mWL CnCO,) ToL1I Rcsidunl Chlorine rm""' Tcmpernrure (0C) 49 of 90 TVA/ Sequoyah Nuclear Plant, OutfaU IOI, Non-treated October 06-13, 2020 Dav 0 Initial 8 14 7.8 310 57 86 24 9 8.25 8.0 283 25.0 8.23 8.0 254 25 0 8.20 8.0 199 25.0 8.17 8.0 174 25.0 8.16 8.0 149 61 58 <0.10 25.0 816 8.0 155 61 58 <O.l O 25.0 Ceriotlnphnin rlubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Dav I Dav2 Day 3 final lniti.1I final Initial final lni1ial Finni 8.00 7 99 8.04 8.06 7,98 8.02

8. 10 8.2 7.9 8.0 7.S 7.9 7,9 7.8 327 317 3 17 59 84 25.1 24.8 25.0 24.8 25.2 24.9 25.2 8.00 8.03 8.06 8.12 799 8.07 8.12 S.2 8.0 8.1 7.9 79 8.0 7.8 292 293 293 24.9 14.9 24 8 24.9 25.0 25.0 24.9 7.99 8.04 8.06 8.13 8.00 8.08

8. 12 8.2

8. 1

8. 1 8.0 7,9 8.0 7.9 262 165 267 24.9 24.8

.N.8 24.9 25.0 15.0 24.9 7.99 8.02 8.07 8.12 8.02 8.08 814 8.2 l!.1 8.2 8.0 79 8.0 7.9 2 14 201 209 24.9 24,9 24.9 24.9 2 4.9 25.0 24 9 7.99 802 8.08 8.08 8.02 809

8. 15 8.3 81 8.2 8.1 79 8.0 8.0 184 180 183 25.2 25.0

25. 1 25.0 24.9 25.0 25.1 7.99 8.02 8.09 8.08 8.03 8.09 8 16 8.3 S.J 8.2

8. 1 7 9 7.9 8.0 155 154 157 61 64

<0. 10 24 8 25.0 25.1 25.0 24.9 25.0 24 8 7.99 8.02 8.09 8.11 8.03 8 06 8 16 8.3 8.1 8.2 8.2 8.0 80 8 I I 55 155 157 63 62 <0. 10 25.0

25. 1 25.0 25.0 25.0 24.8 25.0 Day 4 Initial 8.20 7.8 105 59 82 24 8 8 23 8.0 275 24.8 8 22 8.0 252 24.8 8.:?I 8.0 242 24.8 8.22 S.O 173 24.9

8. 18 8.0 1q5 61 58

<O IO 25 0 8.17 80 141 59 60 <0,10 25 I final 8.06 79 24.9 8.07 7.8 25.2 8.07 7.8 24.8 8.08 7 8 24.9 8 10 7.9 24 9 8.08 8.0 25.0 8.08 8.1 25.0 Project number: 15420 Dav 5 Day 6 Initial final Initial Final 8.19 8.07 8.08 8.07 7.8 7.9 7.8 8.0 JOG 3 16 24.8 25.1 24.8 25.0 8.19 8.08

8. 14 8 04 7.9 7.9 7,9 7.9 274 287 24.9 24.8 24.9 25.2 8.17 8.08 8 15 8.03 7.9 79 79 8 0 254 263 24.9 24.8 24.9 25.3 8.15 8.07 8.15 8.01 8 0 8.0 7.9 8.0 245 24S 24.9 24.9 24.9 25.2

8. 16 8 08 8.13 8 01 8.0 8.0 7.9 8 I 178 ISO 24.9 24 8 25 0 25 2

8. 11 8.09

8. 12 8.02 8 I 8.0 7.9 S I 154 151 25.0 25.0 25 0 25.4 8.10 8 08 8 I I 8.00

8. 1 8 I 8.0

8. 1 149 154 24.8
-2S.2

~4.9 25 I File: sqn101_ 100620chem-correclea Enlered by: T.Sleeper Reviewed by.+ Page IDf> Page _l_ of _I_ Total Residual Chlorine (4500-CI G-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L Analyst I llv ~> Date analyzed l*o, QlP. W DPO: j INR '1Bi;' Positive and Negative Control: Control Type Sample ID Result( ) Positive Negative Negative control Deionized water Positive control Tap water ~ Sample screening: Sample Sample ID Sample characteristics Result( ) number (color - clarity - particles - odor) Positive Negative

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,~/>\\ I $$.N 10 \\ ~o,010,,.. (,'a_r+.;c.1.-,. r.**~. r '1..0 I OO"S.Q\\.:, 1 '~"'\$,,e 'Po.\\l. v, 1\\0"-) Lll't>.r P~<-\\-: <, ll.<:. IO IO 05. 0 \\ '"" I w~tJ 10\\ I i<K\\,i+-Vo ltoaj C..lto.r Pa.-r+;,-1,-,c 1 I ~ 1 1..o 100C:::. ~'- ll*JTP\\V',e_ \\ ~"I ri+ \\/ t!'...110 V0 c.1 eo. ~ ?o.,r+*,*\\e<:. '"l.0 l\$l"\\~, IC> -r~"'lw&..,) \\\\~ l io,h~ \\JP \\\\nl,.A.) c.;l(Ar, P~r-k,: te c;. V -i.o, oos.. o--\\ lr ,~w..e. '04 \\(. "' \\\\m.l C\\(t>.r Po,,r-tt r If"'>

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o,.,\\11- \\In lln.-. , \\ec;,..r I 10,001.- ot r,;;Ave.tJ Co. l;,.A,..\\-,1, 1 tn,., t ' In....lo.A.. LO I UOI.- OC\\ ,hA.(u) C {\\. i::-~ IP V/1.lln,?, t: \\oll'()\\."Y' v"""' 1..0100C.. -\\\\ iP--vr li(e.-.t.e \\ ;4,n.\\- Ve.l\\ow e, llo.r r'o.r+-:~ \\le.... v-7.J:llt> O \\a. \\1-(.\\.[_ 1 O,}..Ot' )(QM.~ 1o roi.-,1,...., r \\01.1*ot"'- ?o.A..:.'-\\e5 ft.\\-~\\c!"'"' 1.01001..,o NC.., lxrr 12.row" , C..lfo.r f'o, r.\\:-,,. te..\\ / J0*0<- *1,c,) ~ Note: All samplC?s were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. A positive result Indicates the presence of total residual chlorine above detection (> 0.10 mg/L), which results In a pink to red color change with the addition of the DP0 indicator. A negative result indicates the absence of total residual chlorine, which results in no color change with the addition of the 0PD indicator. Reviewed by I f Date reviewed===1:0:-=Q=\\.=* :i:o==== 50 of 90 SOP CB-Revision 5-Exhibit CS 1 0 I I I I Page 3 ~ Page _ l_ of _ J _ Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL = 0.05 mg/L, Meter: Accumet Model AB250, SN 92349123 Analyst I~$ Date analyzed.1-,---'o'-. O-=--<e---J-0---t Iodide reagent: INR '?i'I? Acid reagent: INR JOO i Calibration: 0.05 mg/l 0.50 mg/l 5.00 mg/L Slope Values (mV) (s1Jggested range= 26 to 30 mV) Reference standard II INSS f ~'69 INSS I~~ INSS liiq _,, <l' I

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Note: For samples with a residual chlorine of > 1.0 mg/L, the samples must be diluted to be within the calibration range. Laboratory control sample: Refer,enc-e standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/l) (mg/L) (acceptable range = 90 to 110%) INSS fSSl:l 0.25 o.aso /00. 0 °/o Duplicate sample precision: Sample n umber Sample ID Total residual chlorine %RPO= {(S - D) /[(S+D)/2]} x 100 (mg/L) {acceptable range =+/- 10%) L-C.':, \\ oo ~- ti C::.. if~",<5.~N H} \\ s,o.oo'-\\ Duplicate D 1..0, co3 _::t~ lQ, 0 ti

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Sample measurements: Sample Sample ID Total residual chlorine number (mg/l) TV < 0.025 mg/L Method Blank (MB) Lo.oar/> .., l\\\\\\)0~-C\\;. T-J ~ \\ <;. £h,.j 11,.),.. I\\. \\<.e_ '-0.00~ ,_o ICC)S,O \\ ,..J p.' l \\UP-.N It> l .t.Q.00~ 1...Q \\ 00 $, (fl.. t I I..C.00 l IN'T1ll.K~ '1..C \\ OClS

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,~fl,.\\u:)~~ I\\°:) '-0,Co ~ ..,. r. lOO~.o...\\. ..\\,' ,...:i-r-.P>.~ c!. 0.00 l L ()IDOi., O'-( \\'>-t (A'J.,Je.f\\ t:o,oo 1 V ~ ~ I'\\/"\\ ,.., n ~ *;;:::---- .....-"h ~ V" Note: All samples were analvzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS = MV / TV J< 100 number (mg/L) (mg/L) (acceptable range= 90 to ll0%) INSS J8'8<} 0.25 f"'l. ~a eo 94.4 °/o Reviewed by l._ __ ~_1 __ __, Date reviewed I /fr Oli* 1. o 51 of 90 SOP CS-Revision 5-Exhibit (8_2 £ntfflMl!NnnlT*nlnos.lvdon1,ln<. Page II I Page _/_ of_/_ Total Residual Chlorine (4500-CI G-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 rng/L Analyst,~S Date analy,ed '=-Oli'-.:1Q DPD: j 1NR '1~5 Positive and Negative Control: Control Type Sample ID Result{ ) Positive Negative Negative control Deionized water Positive control Tap water Sample screening: Sample Sample ID Sample characteristics Flesult ( ) number (color - clarity - particles - odo1) Positive Negative

7. O 1001,,. "'I....

~ ,..l *U lf l°\\-JI\\ \\<;Q.~ IQ\\ nc co lot\\ c \\t.ar \\/" "2.0l~(f\\,1.~ l, \\~\\<.e.. J,~h-1 -t GI n, dc.e;. r v--- '7...tllO<f\\, i.-\\ ""\\'VI\\ \\..Jl!>t..i 10\\ h5ht tc'.4n I c.l c.ar \\/'" 1..0ll:l\\f\\,"\\.S l I \\~f',~ (\\0 co /or, c. \\eeic-v '"'t()\\ ~1.)\\.1.l,. ""\\\\J/1.. \\ l)J~ \\\\~ /18ht -rein, c lc.~r ....,/ J r..lC'llr\\. "\\....\\ l ' ,~~\\t..L no co /or, c. \\e.c.r v' "'\\...010~~. 1)1..- fl~P--'TT"f'lt-l OC:61\\ oro.r,~e.. 1 Clc:CI< 1.Cltlb i

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~ f),. i-tell f\\~ ()O color, c ie?.41 , (),ooJ'. o~ ( A.*-'*.P \\..eTev tJ e.. f')o color, c:lc.ctr V "1..oro o.f. oS \\ 'i, (flj\\.JW I 1 ~ h + '1 d l o v-l, <- \\ CLO..c -i.o 1oor. 01o K" I ~\\..A t-.Jt:::> , lflh+ ')tllow 1 cko.r LO,ooe. n ~\\j._~ no c.o /or I c. \\co,_ ""2.b,oa..t. t1~ cu& 1151-)-t-ye.l\\o~, cko.r -, ntC>0...E,OC\\ UU\\~ C.o. /15t-it 'fc.110\\..J, cle..o.r, parhc. \\~s 1n1 ()n.t-. JO 'bt,.,<t..e_ Co. /1511+- ye..llow 1 cko.r* 1..o I 00 {!-. I\\ wov'l,.S\\.e.'i no color 1 clc.0ir, f~r--nc.k~ / "'LO I oof. l~ Mb ~eA.1::) ye../fol.,J, cl~r I po.r-hc. lc..S 1 n 1M~. I~ ( Af1~ °PouJefl.. f\\O color, c/c.ar ) ~Clf11(. \\ C: ~ ,1>1 ooJ>,,1..,. CJ-\\e~~ ~~e... no color I clc.c.r

-tS IC). nR. ;}0 Nole: All samples were analyzed in excess ol EPA recommended holding time (15 minutes) unless otherwise noted. A positive result indicates the presence of total residua! chlorine above detection (> 0.10 mg/L), which results in a pink to red color change with the addition of the DPD indicator.

A negative result indicates the absence of total residual chlorine. which results in no color change with the addition of the DPO indicator. Reviewed by I J{ Date reviewed ~===1:0:* o:e:*:2:<J==== 52 of90 SOP CB-Revision 5-Exhibit C8.l}}

ML20350B706

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