ML20350B704

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Discharge Monitoring Report (Dmr), October 2020, Part 1 of 3
ML20350B704
Person / Time
Site: Sequoyah  Tennessee Valley Authority icon.png
Issue date: 11/12/2020
From: Markum T
Tennessee Valley Authority
To:
Document Control Desk, Office of Nuclear Reactor Regulation
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Download: ML20350B704 (32)


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{{#Wiki_filter:[I!] Tennessee Valley Authority, Sequoyah Nuclear Plant, P.O. Box 2000 , Soddy Daisy, TN 37384 November 12, 2020 ATTN : Document Control Desk U.S. Nuclear Regulatory Commission Washington , D.C. 20555-0001

Subject:

Sequoyah Nuclear Plant, Discharge Monitoring Report (DMR), October 2020 Attached is the October 2020 DMR for Sequoyah Nuclear Plant. Respectfully, Travis R. Markum Environmental Scientist

DMR Copy of Record Permit Permit #: TN0026450 Permittee: Tennessee Valley Authority (TVA) Facility: TVA SEQUOYAH NUCLEAR PLANT (SQN) Major: Yes Permittee Address: Sequoyah Access Road, PO Box 2000 Facility Location: SEQUOYAH ACCESS ROAD Soddy Daisy, TN 37379 SODDY DAISY, TN 37379 Permitted Feature: 101 Discharge: 101-G External Outfall (no description) Report Dates & Status Monitoring Period: From 10/01/20 to 10/31/20 DMR Due Date: 11/15/20 Status: NetDMR Validated Considerations for Form Completion Primary discharge from Diffuser Pond. Principal Executive Officer First Name: Scott

Title:

Interim Site Vice President Telephone: 423-843-7001 Last Name: Hunnewell No Data Indicator (NODI) Form NODI: -- I Code Parameter Name Monitoring Location Season Param. NODI Sample Qualifier 1 Value 1 Quantity or Loading Qualifier 2 Value 2 Units Qualifier 1 Value 1 Quality or Concentration Qualifier Value Qualifier 2 2

                                                                                                                                                                                                                                   =

3 36.8 Value 3 Units 04 - deg C

                                                                                                                                                                                                                                                                                    # of Ex.

Frequency of Analysis 99/99 - Continuous Sample Type RC - Recorder (auto) I Permit 04 - deg RC - Recorder 00010 Temperature, water deg. centigrade 1 - Effluent Gross 0 -- Req. Req Mon DAILY MX C 0 99/99 - Continuous (auto) Value NODI 04 - deg Sample = 23.3 99/99 - Continuous CA - CALCTD C Z - Instream Permit 04 - deg 00010 Temperature, water deg. centigrade 0 -- Req.

                                                                                                                                                                                                                                   <=        30.5 DAILY MX C

0 99/99 - Continuous CA - CALCTD Monitoring Value NODI 04 - deg Sample = 2.3 99/99 - Continuous CA - CALCTD C Permit 04 - deg 00016 Temp. diff. between samp. & upstrm deg. C 1 - Effluent Gross 1 -- Req.

                                                                                                                                                                                                                                   <=        3.0 DAILY MX C

0 99/99 - Continuous CA - CALCTD Value NODI RC - Recorder Sample = 1786.6 = 1915.5 03 - MGD 99/99 - Continuous (auto) Permit Req Mon MO Req Mon DAILY RC - Recorder 50050 Flow, in conduit or thru treatment plant 1 - Effluent Gross 0 -- Req. AVG MX 03 - MGD 0 99/99 - Continuous (auto) Value NODI Sample Permit 19 -

                                                                                                                                                                    <=        0.027 MO AVG                                         <=        0.047 DAILY MX                              01/07 - Weekly     GR - GRAB Req.                                                                                                                                                                      mg/L 50060    Chlorine, total residual                       1 - Effluent Gross    0      --

Value B - Below Detection Limit/No B - Below Detection Limit/No NODI Detection Detection 62 - deg Sample = 0.9 99/99 - Continuous CA - CALCTD C/hr Z - Instream Permit 62 - deg 82234 Temperature rate of change deg. C/hr 0 -- Req.

                                                                                                                                   <=        2.0 DAILY MX C/hr 0    99/99 - Continuous CA - CALCTD Monitoring Value NODI Sample                                                               >         100.0                                                                                        23 - %        01/30 - Monthly    CP - COMPOS Permit IC25 Static Renewal 7 Day Chronic                                                                                                                          >         69.0 MINIMUM                                                                                 23 - %        01/30 - Monthly    CP - COMPOS TRP3B                                                   1 - Effluent Gross    0      --          Req.                                                                                                                                                                               0 Chrceriodaphnia Value NODI Sample                                                               >         100.0                                                                                        23 - %        01/30 - Monthly    CP - COMPOS Permit IC25 Static Renewal 7 Day Chronic                                                                                                                          >         69.0 MINIMUM                                                                                 23 - %        01/30 - Monthly    CP - COMPOS TRP6C                                                   1 - Effluent Gross    0      --          Req.                                                                                                                                                                               0 Chrpimephales Value NODI Submission Note If a parameter row does not contain any values for the Sample nor Effluent Trading, then none of the following fields will be submitted for that row: Units, Number of Excursions, Frequency of Analysis, and Sample Type.

Edit Check Errors No errors.

Comments Method Detection Limit for Total Residual Chlorine is 0.05 mg/L. Attachments Name Type Size SQN_2020_October_WET_Report_for_DSN101.pdf pdf 8415687.0 Report Last Saved By Tennessee Valley Authority (TVA) User: TRMARKUM Name: Travis Markum E-Mail: trmarkum@tva.gov Date/Time: 2020-11-10 12:48 (Time Zone: -06:00) Report Last Signed By User: SWHUNNEW Name: Scott Hunnewell E-Mail: swhunnewell@tva.gov Date/Time: 2020-11-12 11:08 (Time Zone: -06:00)

DMR Copy of Record Permit Permit #: TN0026450 Permittee: Tennessee Valley Authority (TVA) Facility: TVA SEQUOYAH NUCLEAR PLANT (SQN) Major: Yes Permittee Address: Sequoyah Access Road, PO Box 2000 Facility Location: SEQUOYAH ACCESS ROAD Soddy Daisy, TN 37379 SODDY DAISY, TN 37379 Permitted Feature: 103 Discharge: 103-G Internal Outfall (no description) Report Dates & Status Monitoring Period: From 10/01/20 to 10/31/20 DMR Due Date: 11/15/20 Status: NetDMR Validated Considerations for Form Completion Internal Monitoring Point for various flows from Low Volume Waste Treatment Pond to Diffuser Pond, which eventually discharges through Outfall 101. Principal Executive Officer First Name: Scott

Title:

Interim Site Vice President Telephone: 423-843-7001 Last Name: Hunnewell No Data Indicator (NODI) Form NODI: -- I Code Parameter Name Monitoring Location Season # Param. NODI Sample Qualifier 1 Value 1 Quantity or Loading Qualifier 2 Value 2 Units Qualifier 1

                                                                                                                                                                                         =           7.4 Value 1 Quality or Concentration Qualifier 2     Value 2  Qualifier 3
                                                                                                                                                                                                                                          =           7.4 Value 3     Units 12 - SU
                                                                                                                                                                                                                                                                               # of Ex. Frequency of Analysis Sample Type 01/30 - Monthly       GR - GRAB I

Permit Req. >= 6.0 MINIMUM <= 9.0 MAXIMUM 12 - SU 01/30 - Monthly GR - GRAB 00400 pH IM - Internal Monitoring Point 0 -- 0 Value NODI Sample = 13.8 = 13.8 19 - mg/L 01/30 - Monthly GR - GRAB Permit Req. <= 30.0 MO AVG <= 100.0 DAILY MX 19 - mg/L 01/30 - Monthly GR - GRAB 00530 Solids, total suspended IM - Internal Monitoring Point 0 -- 0 Value NODI Sample < 5.0 < 5.0 19 - mg/L 01/30 - Monthly GR - GRAB Permit Req. <= 15.0 MO AVG <= 20.0 DAILY MX 19 - mg/L 01/30 - Monthly GR - GRAB 00556 Oil & Grease IM - Internal Monitoring Point 0 -- 0 Value NODI Sample = 1.338 = 1.381 03 - MGD 01/30 - Monthly IN - INSTAN Permit Req. Req Mon MO AVG Req Mon DAILY MX 03 - MGD 01/30 - Monthly IN - INSTAN 50050 Flow, in conduit or thru treatment plant IM - Internal Monitoring Point 0 -- 0 Value NODI Submission Note If a parameter row does not contain any values for the Sample nor Effluent Trading, then none of the following fields will be submitted for that row: Units, Number of Excursions, Frequency of Analysis, and Sample Type. Edit Check Errors No errors. Comments Attachments No attachments. Report Last Saved By Tennessee Valley Authority (TVA) User: TRMARKUM Name: Travis Markum E-Mail: trmarkum@tva.gov Date/Time: 2020-11-10 12:21 (Time Zone: -06:00) Report Last Signed By User: SWHUNNEW Name: Scott Hunnewell E-Mail: swhunnewell@tva.gov Date/Time: 2020-11-12 11:08 (Time Zone: -06:00)

DMR Copy of Record Permit Permit #: TN0026450 Permittee: Tennessee Valley Authority (TVA) Facility: TVA SEQUOYAH NUCLEAR PLANT (SQN) Major: Yes Permittee Address: Sequoyah Access Road, PO Box 2000 Facility Location: SEQUOYAH ACCESS ROAD Soddy Daisy, TN 37379 SODDY DAISY, TN 37379 Permitted Feature: 107 Discharge: 107-G Internal Outfall (no description) Report Dates & Status Monitoring Period: From 10/01/20 to 10/31/20 DMR Due Date: 11/15/20 Status: NetDMR Validated Considerations for Form Completion Metal Cleaning Waste Pond discharge. No monitoring required for stormwater decanting. Daily monitoring required only during dewatering events. Principal Executive Officer First Name: Scott

Title:

Interim Site Vice President Telephone: 423-843-7001 Last Name: Hunnewell No Data Indicator (NODI) Form NODI: -- I Code Parameter Name Monitoring Location Season # Param. NODI Sample Quantity or Loading Qualifier 1 Value 1 Qualifier 2 Value 2 Units Qualifier 1 Value 1 Quality or Concentration Qualifier 2 Value 2 Qualifier 3 Value 3 Units

                                                                                                                                                                                                                                            # of Ex. Frequency of Analysis Sample Type I

Permit Req. >= 6.0 MINIMUM <= 9.0 MAXIMUM 12 - SU 01/01 - Daily GR - GRAB 00400 pH IM - Internal Monitoring Point 0 -- Value NODI C - No Discharge C - No Discharge Sample Permit Req. <= 30.0 MO AVG <= 100.0 DAILY MX 19 - mg/L 01/01 - Daily CP - COMPOS 00530 Solids, total suspended IM - Internal Monitoring Point 0 -- Value NODI C - No Discharge C - No Discharge Sample Permit Req. <= 15.0 MO AVG <= 20.0 DAILY MX 19 - mg/L 01/01 - Daily GR - GRAB 00556 Oil & Grease IM - Internal Monitoring Point 0 -- Value NODI C - No Discharge C - No Discharge Sample Permit Req. <= 1.0 MO AVG <= 1.0 DAILY MX 19 - mg/L 01/01 - Daily CP - COMPOS 01042 Copper, total [as Cu] IM - Internal Monitoring Point 0 -- Value NODI C - No Discharge C - No Discharge Sample Permit Req. <= 1.0 MO AVG <= 1.0 DAILY MX 19 - mg/L 01/01 - Daily CP - COMPOS 01045 Iron, total [as Fe] IM - Internal Monitoring Point 0 -- Value NODI C - No Discharge C - No Discharge Submission Note If a parameter row does not contain any values for the Sample nor Effluent Trading, then none of the following fields will be submitted for that row: Units, Number of Excursions, Frequency of Analysis, and Sample Type. Edit Check Errors No errors. Comments Attachments No attachments. Report Last Saved By Tennessee Valley Authority (TVA) User: TRMARKUM Name: Travis Markum E-Mail: trmarkum@tva.gov Date/Time: 2020-11-10 12:21 (Time Zone: -06:00) Report Last Signed By User: SWHUNNEW Name: Scott Hunnewell E-Mail: swhunnewell@tva.gov Date/Time: 2020-11-12 11:08 (Time Zone: -06:00)

DMR Copy of Record Permit Permit #: TN0026450 Permittee: Tennessee Valley Authority (TVA) Facility: TVA SEQUOYAH NUCLEAR PLANT (SQN) Major: Yes Permittee Address: Sequoyah Access Road, PO Box 2000 Facility Location: SEQUOYAH ACCESS ROAD Soddy Daisy, TN 37379 SODDY DAISY, TN 37379 Permitted Feature: 110 Discharge: 110-G External Outfall (no description) Report Dates & Status Monitoring Period: From 10/01/20 to 10/31/20 DMR Due Date: 11/15/20 Status: NetDMR Validated Considerations for Form Completion Outfall 110 is closed. Only active in the event the plant goes into closed mode. Principal Executive Officer First Name: Scott

Title:

Interim Site Vice President Telephone: 423-843-7001 Last Name: Hunnewell No Data Indicator (NODI) Form NODI: -- I Code Parameter Name Monitoring Location Season # Param. NODI Sample Qualifier 1 Value 1 Quantity or Loading Qualifier 2 Value 2 Units Qualifier 1 Value 1 Quality or Concentration Qualifier 2 Value 2 Qualifier 3 Value 3 Units

                                                                                                                                                                                                                                                                                               # of Ex. Frequency of Analysis    Sample Type I

Permit Req. Req Mon DAILY MX 04 - deg C 99/99 - Continuous CA - CALCTD 00010 Temperature, water deg. centigrade 1 - Effluent Gross 0 -- Value NODI C - No Discharge Sample Permit Req. <= 30.5 DAILY MX 04 - deg C 99/99 - Continuous CA - CALCTD 00010 Temperature, water deg. centigrade Z - Instream Monitoring 0 -- Value NODI C - No Discharge Sample Permit Req. <= 3.0 DAILY MX 04 - deg C 99/99 - Continuous CA - CALCTD 00016 Temp. diff. between samp. & upstrm deg. C 1 - Effluent Gross 1 -- Value NODI C - No Discharge Sample Permit Req. Req Mon MO AVG Req Mon DAILY MX 03 - MGD 99/99 - Continuous RC - Recorder (auto) 50050 Flow, in conduit or thru treatment plant 1 - Effluent Gross 0 -- Value NODI C - No Discharge C - No Discharge Sample Permit Req. <= 0.027 MO AVG <= 0.047 DAILY MX 19 - mg/L 05/WK - Five Per Week GR - GRAB 50060 Chlorine, total residual 1 - Effluent Gross 0 -- Value NODI C - No Discharge C - No Discharge Sample Permit Req. <= 30.5 DAILY MX 62 - deg C/hr 99/99 - Continuous CA - CALCTD 82234 Temperature rate of change deg. C/hr Z - Instream Monitoring 0 -- Value NODI C - No Discharge Sample Permit Req. >= 69.0 MINIMUM 23 - % 01/30 - Monthly GR - GRAB TRP3B IC25 Static Renewal 7 Day Chronic Chrceriodaphnia 1 - Effluent Gross 0 -- Value NODI C - No Discharge Sample Permit Req. >= 69.0 MINIMUM 23 - % 01/30 - Monthly GR - GRAB TRP6C IC25 Static Renewal 7 Day Chronic Chrpimephales 1 - Effluent Gross 0 -- Value NODI C - No Discharge Submission Note If a parameter row does not contain any values for the Sample nor Effluent Trading, then none of the following fields will be submitted for that row: Units, Number of Excursions, Frequency of Analysis, and Sample Type. Edit Check Errors No errors. Comments Attachments No attachments. Report Last Saved By Tennessee Valley Authority (TVA) User: TRMARKUM Name: Travis Markum E-Mail: trmarkum@tva.gov Date/Time: 2020-11-10 12:21 (Time Zone: -06:00)

Report Last Signed By User: SWHUNNEW Name: Scott Hunnewell E-Mail: swhunnewell@tva.gov Date/Time: 2020-11-12 11:08 (Time Zone: -06:00)

DMR Copy of Record Permit Permit #: TN0026450 Permittee: Tennessee Valley Authority (TVA) Facility: TVA SEQUOYAH NUCLEAR PLANT (SQN) Major: Yes Permittee Address: Sequoyah Access Road, PO Box 2000 Facility Location: SEQUOYAH ACCESS ROAD Soddy Daisy, TN 37379 SODDY DAISY, TN 37379 Permitted Feature: 118 Discharge: 118-G External Outfall (no description) Report Dates & Status Monitoring Period: From 10/01/20 to 10/31/20 DMR Due Date: 11/15/20 Status: NetDMR Validated Considerations for Form Completion Principal Executive Officer First Name: Scott

Title:

Interim Site Vice President Telephone: 423-843-7001 Last Name: Hunnewell No Data Indicator (NODI) Form NODI: -- I Code Parameter Name Monitoring Location Season # Param. NODI Sample Qualifier 1 Value 1 Quantity or Loading Qualifier 2 Value 2 Units Qualifier 1 Value 1 Quality or Concentration Qualifier 2 Value 2 Qualifier 3 Value 3 Units

                                                                                                                                                                                                                                                                             # of Ex. Frequency of Analysis Sample Type I

Permit Req. >= 2.0 MINIMUM 19 - mg/L 02/07 - Twice Every Week GR - GRAB 00300 Oxygen, dissolved [DO] 1 - Effluent Gross 0 -- Value NODI C - No Discharge Sample Permit Req. <= 100.0 DAILY MX 19 - mg/L 02/07 - Twice Every Week GR - GRAB 00530 Solids, total suspended 1 - Effluent Gross 0 -- Value NODI C - No Discharge Sample Permit Req. <= 1.0 DAILY MX 25 - mL/L 01/30 - Monthly GR - GRAB 00545 Solids, settleable 1 - Effluent Gross 0 -- Value NODI C - No Discharge Sample Permit Req. Req Mon MO AVG Req Mon DAILY MX 03 - MGD 01/BA - Once Per Batch ES - ESTIMA 50050 Flow, in conduit or thru treatment plant 1 - Effluent Gross 0 -- Value NODI C - No Discharge C - No Discharge Submission Note If a parameter row does not contain any values for the Sample nor Effluent Trading, then none of the following fields will be submitted for that row: Units, Number of Excursions, Frequency of Analysis, and Sample Type. Edit Check Errors No errors. Comments Attachments No attachments. Report Last Saved By Tennessee Valley Authority (TVA) User: TRMARKUM Name: Travis Markum E-Mail: trmarkum@tva.gov Date/Time: 2020-11-10 12:15 (Time Zone: -06:00) Report Last Signed By User: SWHUNNEW Name: Scott Hunnewell E-Mail: swhunnewell@tva.gov Date/Time: 2020-11-12 11:08 (Time Zone: -06:00)

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT lNTROD UCTlON / EXECUTIVE

SUMMARY

Report Date: November 06. 2020 I. acility / Discharger: Sequoyah Nuclear Plant / TY A 2 County / State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450
4. Type of Facility: Nuclear-Fueled Electric Generating Plant
5. Design Flow (MGD) : 1.579
6. Receiving Stream: Tennessee River (TRM 483.6)
7. IQl0: 2-456
8. Outfall Tested: 101
9. Dates Sampled: October 04 -09, 2020 l 0. Average Flow on Days Samp led (MOD) : l 791 .1 3. 1770.6 l 8, 1783.065 l l . Pertinent Site Conditions: Production/ operation data will be provided upon request.
12. Test Dates : October 06 - 13, 2020 13 . Test Type : Sho1t-term Chronic Definitive
14. esl Spec ies: Fathead Mitrnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested(%) :

Pimephales prornelas: UV treated Outfall l 01: l 7.25. 34.5. 69. 84 .5. I00 UV treated Intake: I 00 Cenodaphnia dubia: Non-treated Outfall 10 l: 17.25. 34 .5. 69. 84.5. l 00 Non-treated Intake: 100

16. Permit Limit Endpoint (o/t ): Outfall l Ol: lC2s = 69%
17. Test Results : Outfall 101 : Pimephalespromelas: IC2s> 100%

eriodaphnia dubia: IC2s > 100% 1 of90

18. Facility

Contact:

Travis Markum Phone#: (865) 748-3294

19. Consulting/ Testing Lab: Environmental Testing Solutions, Inc .
20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TV A

Contact:

Rick Sherrard Phone #: (423) 876-6 743

22. Notes: Exposures to samples collected October 04 - 09, 2020 from Outfall I 01 re ulted in no toxic effects to fathead minnows or daplmids. The re ulting IC2s values, for both species were> l 00 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Minnow growth in the intake samples wa significantly lower than the control.

2of90

METHODS

SUMMARY

Samples : I. Sampling Point: Outfall l 0], Intake

2. Sample Type : Composite
3. Sample lnfonnation:

Date Date Date (MM-DD-YY) (MM- DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (OC) (mg/L) Last Used By l 0-04-20 / 0700 lo I0-06-20 / 0925 IOI 10-05-20 I 0600 10-05-20 I 1420 1.0 <0.10 I0-07-20 / 0853 I0-04-20 / 0700 to I0-06-20 / 0925 Intake I 0-05-20 / 1420 2.0 <0.10 I0-05-20 / 0600 I0-07-20 I 0853 I0-06-20 I 0700 to I 0-08-20 I 0858 10 l I0-07-20 / 1337 I. I <0.10 I0-07-20 I 0600 I0-09-20 I 0857 I0-06-20 / 0700 to I0-08-20 / 0858 Intake 10-07-20 / 133 7 I. 7 <0. 10 I0-07-20 / 0600 I0-09-20 / 085 7 10-10-20 / 0907 I0-08-20 I 0700 to 101 10-09-20 / 1305 1.7, 2.l t <0. 10 10-11-20 / 0856 I0-09-20 / 0600 I 0- I 2-20 / 0828 I 0- 10-20 I 0907 I0-08-20 / 0700 to rntake I 0-09-20 I 0600 l 0-09-20 / 1305 2.0 <0.10 l 0-11-20 / 0856 I0- 12-20 / 0828

  *TRC = Total Residual Chlorine tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25. O+/- l. 0°C) in a warm water bath.

Alig uots of Outfall l 0 1 and Intake samples were UV -treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. 3of 90

Pimephales promelas Ceriodaphnia dubia Test Organisms: I. Source: In-house Cultures In-house Culture

2. Age : < 24-hours old < 24-hours old Test Method Summary :
1. Test Conditions: Static. Renewal Static. Renewal
2. Test Duration: 7 days Until at least 60% of control females have 3 broods
3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
4. Number of Replicates : 4 lQ
5. Organisms per Replicate: 1..Q l
6. Test lnitiation: (Date/Time): 10-06-20 0823 ET 10-06-20 0925 ET
7. Test Tennination : (Date/Time): 10-13-20 0732 ET 10-13-20 0837 ET
8. Test Temperature: Outfall 101: Mean = 24.7°C Mean = 25.0°C (24 .2 - 25.2°C) (24 .8 - 25.4°C)
9. Physical / Chemical Measurements: Alkalinity. hardness. total residual chlorine. and conductivity were measured at the laboratory in each I 00% sample. Daily temperatw-es were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

I0. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville, CA). 4 of90

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) I. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type/ Duration) Conducted October 06 - 13. 2020 using effluent from Outfall IO 1. Test Percent Surviving Solutions (time interval used - days) (% Effluent) I 2 3 4 5 6 7 Control, 100 100 100 100 100 100 100 UV-treated 17.25% 100 100 100 100 100 100 100 34.5% 100 lOO 100 100 100 100 100 69% 100 100 100 100 100 100 100 84.5% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 97 .5 Control, 100 100 100 JOO 100 100 100 Non-treated Mean Dry Weight (mg) Test olutions (replicate number) (% Effluent 1 2 ".) 4 Mean Control , 0.775 0.725 0.816 0.879 0.799 UY-treated 17.25% 0.737 0.729 0.745 0.803 0.754 34.5% 0.715 0.700 0.822 0.839 0.769 69% 0.810 0.769 0.761 0.787 0.782 84.5% 0.741 0.745 0.712 0.717 0.729 100.0% 0.765 0.751 0.722 0.757 0.749 Intake 0.634 0.687 0.670 0.701 0.673 Control 0.845 0.790 0.853 0.745 0.808 Non-treated JC2s Value : > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 69% Permit Limit: 1.4 TUc 95% Confidence Limits: Upper Limit : NA Lower Limit: NA

    *TUa :;: l 00/LCso: TUc = 100/ lC2s 5of 90

TOXLCITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Resu lts of a Ceriodaphnia dubia Clu*onic/ 7-dav Toxicity Test.

(Genus species) (Type/ Duration) Conducted October 06 - I 3. 2020 using effluent from OutfaJl 101. Percent Surviving Test (lime interval used - days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control JOO 100 100 100 100 100 100 17.25% 100 100 100 100 100 100 100 34.5% 100 100 100 JOO 100 100 JOO 69% 100 100 100 100 100 100 100 84.5% 100 100 100 100 100 100 JOO 100.0% 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% Effluent) l 2 3 4 5 6 7 8 9 IO Mean Control 29 30 31 28 30 32 32 28 30 33 30.3 17.25% 32 33 35 31 34 30 34 31 32 33 32.5 34.5% 34 33 34 33 35 37 30 32 32 33 33.3 69% 32 38 34 39 35 32 37 33 34 37 35.1 84.5% 40 36 35 35 35 36 34 34 39 35 35.9 100.0% 39 39 37 35 36 41 34 39 36 38 37.4 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Pennit Limit: 69% Permit Limit : I .4 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

      *TU a = 100/LCso: TUc = 100/ IC2s 6 of90

TOXICITY TEST RESULTS (see Appendix for Bench Sheets)

2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration) Conducted October 06 - 13.2020 using water from Intake Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (% Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Test Solutions Data (replicate number) (% E ffluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 26 29 31 32 32 32 29 29 32 30.2 Intake 35 36 32 36 36 35 36 35 35 33 34.9 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A Permit Limit: NIA 95% Confidence Limits : Upper Limit: NA Lower Limit: NA

       *TU a = 100/LCso: TUc = l 00/ IC2s REFERENCE TO XI CANT TEST RES UL TS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC2s) Pimephales promelas October 06 - 13, 2020 0811 7 days KCI 0.65 g/L Ceriodaphnia dubia October 06 - 13, 2020 0913 7 days NaCl l.09 g/L 7of90

PHYSI CA UCHEMI CAL

SUMMARY

Wa1er Chemistry Mean Values nnd Rnnges !or UV- lreatcd Plmeplmles promelos and No11-1rcated Cenodaphma dubm. Sequoyah Nuclear Plant (SQN), Eflluent Outfall 101 ant! lnlnkc pcrlomicd October 06-13.2020 Tesi Sample IO T emperature (°C) Dissolved Oxygen (mi:/L) 11ll (S.U.) Conductance /\llrnlinily M:mlnc ss

  • Total Residual Initial Final Initial Final lnitinl final (µmhos/em) (mg/L CaCOJ) (mg/L CaCOJ) Chlorine (mg/L)

C on 1rnl , 24.8 24 .6 7.8 74 8. 10 7. 80 314 58 84 -

                     ,'10 11 -tr rwfC'd 24.7 - 25.0 24.5 - 25.0 7.8 - 7.9 6.5 - 8.0              7 99 - 8.20 7 57 . 7.9.J 305
  • 327 57 . 59 82 86 Con 1ro l, 24.8 24.6 7.9 7.3 8.09 7.79 306 60 82 -

lJV*hU Ctd 24.7 - 24 9 24,3

  • 24.7 7.8 - 8.0 6.6
  • 79 7.99 - 8. 18 7.59 - 7 94 293 - 314 59 - 60 80 84 .
       ..,              17.25%

24 .8 24.5 8.0 7.3 8. 11 7.79 285 - .

     -S:                                24.7 - 25.0 24.3
  • 24.8 8.0 81 6.7 - 79 8.00 - 8.22 7 57 - 7.94 277 - 293 . .
       ~
       ~

24 .9 24.S 8.1 7.3 8. 11 7.79 256 . - 3-4.5%

       ...,"'-                          247 - 250 24.3 - 24.6 8.0
  • 8.1 6.6
  • 79 8.01 - 8.19 7.58
  • 7.94 241
  • 262 . .
     ~                                      24 .9         24.5           8. 1          7.3            8.10              7.78      223                         .                      .
     ~ "'                  69%

248 - 250 24.3

  • 24.6 81 . 8.2 6 6
  • 80 8.00 - 8 17 7 56
  • 7.93 203 - 248 . . .
       ~

t: 24.9 24.5 8. 1 7.3 8. 10 7.81 179 . i:': 84.5% 24.9 - 25.0 24.3 - 24 7 8. 1 82 6 6 - 80 8.00

  • 8 17 7.64 - 7.97 173 . 184 . .

25.0 24.5 8. 1 7.4 8. 10 7.80 153 60 60 < 0. 10 100% 25.0 . 25.0 24.2 - 24 .6 8 0

  • 8.3 6 9 - 8.0 8.0 1 - 8.16 7.60 - 7.% 148 - 156 59 - 61 58 . 62 <0. 10 - <0. 10 25.0 24.5 8.2 7.4 8 06 7.77 15 1 60 59 < 0. 10 Intake 24.9 - 25.2 24 4 - 24 6 8 1 8.3 6.4
  • 8.0 7.88 - 8. 14 7 53 - 7.93 146
  • 156 59 63 58 62 < 0. 10 - < 0. 10 C oflt,.,ol. 24.8 25. 1 7.8 8.0 8.10 8.05 3 14 58 84 .

Nfl n~1rt~tcd 24 8

  • 24.9 24 9
  • 25.2 7 8
  • 79 7.8
  • 8.2 7.99
  • 820 7.9&
  • 8. 10 305 - 327 57 59 82 86 .

24 9 25.0 8.0 7.9 8. 15 8.05 285 - . 17.25% 24.8 - 25.0 24.8 - 25.2 7 9 - 8.0 7.8

  • 8.2 8.03
  • 8.25 7.99 - 8. 12 274 - 293 . - . .

C

.; 24.9 24.9 8.0 8.0 8. 15 8 OS 260 - . .

3-t53/4

     "'-~                  69%

24.8 - 25.0 24.8 - 25.3 7.9 . 81 24.9 211.9 8.0 7.8 - 82 8.0 8.04 - 8.23 7.99 - 8 12 252 - 267 8.13 8.05 223 .

      ~
        !::-                            24.8 - 25.0 24.9 - 25.2 7 9 - 8. 1        7.8 - 8.2      8.02
  • 8.2 1 7.99 - 8.14 199 - 248 . .

25.0 8.0 8.1 8.12 8.06 179 - .

     -""~
        ...              84.5%

25.0 v 24 9 - 25.0 24.8 - 25 2 7.9 - 8. l 7.9 - 8.3 8 02

  • 8.22 7.9'1 - 8. 15 173 - 184 - .

25.0 25.0 8.0 8. 1 8. 11 8 07 15.2 61 60 <0. 10 100% 25.0 - 25.0 24.8 - 25.4 7. 9 . 8. J 7.9

  • 8.3 802 - 8.18 7.99 - 8. 16 145
  • 157 61 . 6 1 58 64 <0, 10 - <0. 10 25 0 25.0 8. 1 8. 1 8.10 8.06 152 61 60 < 0, 10 Intake 24.8 - 25 I 25 0 - 25 2 SO - 81 8.0 - 8.3 8.02 - 8. 17 7.99 - 8. 16 141 - 157 59 . 6J 58 - 62 < 0 10 - <0. 10
  • Note Total residual chlor/llC was pc rfonned on non-1rca1ed Outfall IOI and Inta ke samples Oven1ll 1cmpcmturc (°C) A,,cn1gc Minimum Maximum P1mephales promelas 24 7 24.2 25.2 Cenodaphnta dub,a 25.0 24.8 25.4 Bof90

SUMMARY

I CO CLUSIONS Exposures to samples collected October 04 - 09, 2020 from Outfall I 01 resulted in no toxic effects to fathead minnows or daplu1ids. The resulting IC2s values for both species. were > I 00 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Minnow grovJth in the intake samples was significantly lower than the control. 9 of90

Appendix A ADDITIONAL TOXICITY TEST lNFORMATION

SUMMARY

OF METHODS I . Pimephales promeia Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic djspo able cups, each containing 250-mL of test sol ution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-82 1-R-02-013 (October 2002) usjng ten replicates each containing one test organism, per treatment. Test vessels consisted of 30-mL po lypropylene cups each containing 15-mL of test solution.

DEVIATION I MODIFICATIONS TO TEST PROTOCOL I . Pimephales promelas Samples used in the fathead minnow test were exposed to UV light for two minutes prior to introduction of test organisms. UV treatment is u ed to control interference of fi h pathogens. This treatment method was approved on November 23 20 l 5 by the State of Te1messee in a letter from Jessica Murphy to Terry Cheek, Senior Manager of TV A Water Permits, Compliance, and Monitoring,

2. Ceriodaphnia dubia None DEVIATIONS I MODIFICATIO S TO PRETEST CULTURE OR HOLDING OF TEST ORGANlSMS l . Pimephales promelas None
2. Ceriodaphnia dubia None 10 of 90

PHYSICAL AND CHEMICAL METHODS I. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where app licable .

2. Instruments: All identification service and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
3. Temperatw-e was measured by SM 2550 B-2010.
4. Dissolved oxygen was measured by SM 4500-0 G-2011.
5. The pH was measured by SM 4500-H+ B-2011 .
6. Conductance was measured by SM 2510 B-2011.
7. Alkalinity was measured by M 2320 B-201 l.
8. Total hardness was measw*ed by SM 2340 C-201 l.
9. Total residual chlorine was measured by ORION 97-70-1977.

QUALITY ASSURANC 1 Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation test organism handling during test, and maintaining appropriate test condition were conducted according to the protocol as described in this report and EPA-821-R-02-013 . Any known deviations were noted during the study and are reported herein. R.EFERE CE TOXlCANT TESTS (See Appendix D for control chart infomiation)

1. Test Type: 7~day chronic tests with results expressed as IC2s values in g/L KC] or NaCl.
2. Standard Toxicant: Potassium Chloride (KC! crystalline) for Pimephales promelas.

Sodium Chloride (NaCl crystalline) fo r Ceriodaphnia dub;a.

3. Dilution Water Used : Moderately hard synthetic water.
4. Statistics: Tox ale software Version 5.0 was used for statistical analyses.

11 of 90

REFEREN ES I . NPOES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-82l-R-02-013 (October 2002).
3. Standard Methods for the Examination of Water and Wastewater, 22 nd Edition, 2012.
4. Quality Assurance Program: Standard Operating Procedures, Environ.mental Testing Solutions, Inc (most current version).

12 of90

Sequoyah Nuclear Plant Biomonitoring October 06 - 13, 2020 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling 13 of 90

Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cu pros tat-PF H-130M Nalco H-150M Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L mg/L TRC Phosphate Copolymer DMAD Awle Quat mg/L Quat TRC EO/PO 02/06/2005 - <0.0042 0.028 0.010 - - - - - 02/07/2005 - <0.0116 0.028 0.010 - - - 0.007 - 02/08/2005 - <0.0080 0.028 0.010 - - - - - 02/09/2005 - 0.0199 0.028 0.010 - - - - - 02/ 10/2005 - <0.0042 0.028 0.010 - - - - - 02/11/2005 - 0.0155 0.028 0.010 - - - 0.007 - 06/05/2005 - 0.0063 - - - - - - - 06/06/2005 - 0.0043 - - - - - - 0.037 06/07/2005 - 0.0103 - - - - - - 0.037 06/08/2005 - 0.0295 - - - - - - 0.037 06/09/2005 - 0.0129 - - - - - - - 06/ 10/2005 - 0.0184 - - - - - - - 07/ 17/2005 - 0.0109 0.026 0.009 - - - - - 07/18/2005 - 0.0150 0.026 0.009 - - - - 0.036 07/ 19/2005 - 0.0163 0.026 0.009 - - - - 0.036 07/20/2005 - 0.0209 0.026 0.009 - - - 0.014 0.036 07/21 /2005 - 0.0242 0.026 0.009 - - - - - 07/22/2005 - 0.0238 0.054 0.Dl8 - - - 0.014 - 10/ 30/2005 - 0.0068 - - - - - - - 10/31 /2005 - 0.0112 - - - - - - - 11/01 /2005 - 0.0104 - - - - - - 0.035 11 /02/2005 - 0.0104 - - - - - - 0.036 11 /03/2005 - 0.0117 - - - - - - 0.036 11/04/2005 - 0.0165 - - - - - - 0.035 11/14/2005 - 0.0274 - - - - - - - 11/15/2005 - 0.0256 - - - - - - - 11/16/2005 - 0.0234 - - - - - - - 11/17/2005 - 0.0231 - - - - - - - 11118/2005 - 0.0200 - - - - - - - 11/19/2005 - 0.0116 - - - - - - - 14 of 90

Date Sodium Towerbron PCI.r222 PCL-401 CI.r363 Cu pros tat-PF H-130M Nalco H-150M MSW Hypochlorite mg/L rng/L rng/L rng/L rng/L rng/L 73551 rng/L 101 rng/L TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L TRC EO/PO Phosphate 11/ 12/ LUUo - o.w::,::, - - - - - - - - 11/13/2006 - 0.0068 - - - - - - 0.037 - 11/14/2006 - 0.0143 - - - - - - 0.037 - 11/15/2006 - 0.0068 - - - - - - 0.037 - 11/16/2006 - 0.0267 - - - - - - 0.037 - 11/17/2006 - 0.0222 - - - - - - - - 11 /26/2006 - 0.0188 - - - - - - - - 11/27/2006 - 0.0138 - - - - - - - - 11/28/2006 - 0.0120 - - - - - - - - 11 /29/2006 - 0.0288 - - - - - - - - 11/30/2006 - 0.0376 - - - - - - - - 12/01/2006 - 0.0187 - - - - - - - - 05/28/07 - - - - - - - - - 0.015 05/29/07 - - - - - - - - 0.036 0.015 05/30/07 - 0.0084 - - - - - 0.017 0.036 0.015 05/ 31 /07 - 0.0103 - - - - - - 0.036 0.015 06/01/07 - 0.0164 - - - - - 0.017 0.036 0.015 06/02/07 - 0.0305 - - - - - - - 0.D15 12/02/07 - 0.0241 - - - - - - - - 12/03/07 - 0.0128 - - - - - - - - 12/04/07 - 0.0238 - - - - - - - - 12/05/07 - 0.0158 - - - - - - - - 12/06/07 - 0.0162 - - - - - - - - 12/07/07 - 0.0175 - - - - - - - 04/13/08 - 0.0039 - - - - - - - - 04/14/08 - 0.0124 - - - - - - - - 04/15/08 - 0.0229 - - - - - - - - 04/16/08 - 0.0143 - - - - - - - - 04/17/08 - 0.0120 - - - - - - - - 04/18/08 - 0.0149 - - - - - - - - 10/26/08 - 0.0260 - - - - - - - - 10/27/08 - 0.0151 - - - - - 0.017 - - 10/28/08 - 0.0172 - - - - - - 0.041 - 10/29/08 - 0.0154 - - - - - 0.018 0.041 0.030 10/30/08 - - - - - - - - 0.041 0.030 10/31 /08 - 0.0086 - - - - - - 0.041 0.030 15 of 90

Date Sodium Towerbron PCI.r PCI.r401 Cl.r363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L -PFmg/L mg/L 73551 CT1300 mg/L 101 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg/L Quat mg/L TRC Phosph EO/PO Quat Phosphat<: ate 02/08/09 - 0.0197 - - - - - 0.017 - - - 02/09/09 - 0.0237 - - - - - 0.017 - - - 02/1 0/09 - 0.0104 - - - - - 0.021 - - - 02/1 1/09 - 0.0155 - - - - - 0.oI7 - - - 02/1 2/09 - 0.0106 - - - - - 0.017 - - - 02/1 3/09 - - - - - - - - - - - 05/1 0/09 - 0.0129 - - - - - - - - - 05/1 1/09 - 0.0415 - - - - - - - 0.0446 - 05/1 2/09 - 0.0053 - - - - - - - 0.0396 - 05/13/09 - 0.0049 - - - - - - - 0.0396 - 05/1 4/09 - <0.0141 - - - - - - - 0.0397 - 05/1 5/09 - <0.0160 - - - - - - - - - 11/ D / U'J - u.u2:, - - - - - - - - - 11 /1 6/09 - 0.0152 - - - - - - - - - 11 /1 7/09 - 0.0255 - - - - - - - - - 11 /1 8/09 - 0.0306 - - - - - - - - - 11 /1 9/09 - 0.0204 - - - - - - - - 11 /20/09 - 0.0093 - - - - - - - - - 05/09/1 0 - 0.0192 - - - - - - - - - 05/1 0/1 0 - 0.0055 - - - - - - - - - 05/1 1/1 0 - 0.0100 - - - - - - 0.039 - - 05/1 2/1 0 - 0.0171 - - - - - - 0.039 - - 05/1 3/1 0 - 0.0041 - - - - - 0.039 - - 05/1 4/1 0 - 0.0099 - - - - - - 0.039 - - 16 of90

Date Sodium Towerbron PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Floguard Hypo- mg/L 222 mg/L mg/L -PFmg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly- DMAD Azole Quat mg/L mg/L Quat mg/L mg/L mg/L Phos- mer EO/PO Quat Phos- Phosphat~ TRC phate phate 10/ 31/10 - - - - - - - - - - - - 11 /01/10 - 0.0122 - - - - - - - - - - 11 /02/10 - 0.0112 - - - - - - - - - - 11/03/ 10 - 0.0163 - - - - - - - - - - 11/04/ 10 - 0.0107 - - - - - - - - - - 11/05/ 10 - 0.0132 - - - - - - - - - - 05/01 /2011 - - - - - - - - - - - - 05/02/2011 - - - - - - - - 0.04 - - - 05/03/2011 - - - - - - - - 0.04 - - - 05/04/2011 - 0.0155 - - - - - - 0.04 - - - 05/05/2011 - 0.0179 - - - - - - 0.04 - - - 05/06/2011 - 0.0089 - - - - - - - - - - 11/VO/ LlJI l - U.Ulo~ - - - - - - - - - - 11 /07/2011 - 0.0225 - - - - - - - - - - 11 /08/2011 - 0.0141 - - - - - - - - - - 11/09/2011 - 0.0239 - - - - - - - - - - 11/10/2011 - 0.0242 - - - - - - - - - - 11/11/2011 - 0.0231 - - - - - - - - - - 05/06/2012 - - - - - - - - - - - - 05/07/2012 - - - - - - - - - - - - 05/08/2012 - - - - - - - - 0.041 - - - 05/09/2012 - 0.0145 - - - - - - 0.041 - - - 05/10/2012 - 0.0298 - - - - - - 0.041 - - - 05/11/2012 - 0.0174 - - - - - - - - - - 08/12/2012 - - - - - - - - - - - 0.029 08/ 13/2012 - 0.0256 - - - - - 0.028 0.037 - - 0.029 08/14/2012 - 0.0209 - - - - - - 0.037 - - 0.029 08/15/2012 - 0.0279 - - - - - 0.028 - - - 0.029 08/16/2012 - 0.0076 - - - - - - - - - 0.029 08/17/2012 - 0.0446 - - - - - - - - - 0.032 05/12/2013 - 0.0099 - - - - - - - - - - 05/13/2013 - - - - - - - - - - 0.064 05/14/2013 - 0.0091 - - - - - 0.039 - - - 0.064 05/ 15/2013 - 0.0096 - - - - - 0.039 - - - 0.064 05/ 16/2013 - 0.0229 - - - - - - - - - 0.032 05/17/2013 - 0.0063 - - - - - - - - - 0.032 09/ 15/2013 - - - - - - - - - - - 0.03 09/16/2013 - 0.0072 - - - - - - 0.0379 - - 0.03 09/17/2013 - 0.0107 - - - - - 0.036 0.0379 - - 0.03 09/18/2013 - 0.0217 - - - - - 0.036 0.0379 - - 0.03 09/19/2013 - 0.0172 - - - - - - - - - 0.03 09/20/2013 - 0.0173 - - - - - - - - - 0.03 17of90

Date Sodium Towerbror PCT.r PCL-401 CT.r363 Cu pros tat H-130M Nalco Spectrus H-150M MSW Floguard Hypo- mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly- DMAD Azole Quat mg/L mg/L Quat mg/L mg/L mg/L Phos- mer EO/PO Quat Phos- Phosphate TRC ohate ohate 05/04/2014 - 0.0118 - - - - - - - - - - 05/05/2014 - 0.0112 - - - - - - - - - - 05/06/2014 - 0.0096 - - - - - - - - - - 05/07/2014 - 0.0164 - - - - - - - - - - 05/08/2014 - 0.0235 - - - - - - - - - - 05/09/2014 - 0.0110 - - - - - - - - - - 09/07/2014 - - - - - - - - - - - - 09/08/2014 - - - - - - - - 0.04 - - - 09/09/2014 - - - - - - - - 0.04 - - - 09/ 10/2014 - - - - - - - - 0.04 - - - 09/11 /2014 - 0.0070 - - - - - - - - - - 09/12/2014 - 0.0074 - - - - - - - - - - UlS/ U~/ LUJ5 - - - 08/ 10/2015 0.0195 0.03 - 08/11 /2015 0.0275 0.03 - 08/12/2015 0.0213 - 0.03 08/13/2015 0.0192 - 0.03 08/ 14/2015 0.0182 - 0.03 10/18/2015 0.0162 10/19/2015 0.0125 10/20/2015 0.0120 10/21/2015 0.0130 10/22/2015 0.0174 10/23/2015 0.0156 05/ 15/2016 - 05/16/2016 0.0209 05/17/2016 0.0210 05/ 18/2016 0.0361 05/19/2016 0.0254 05/20/2016 0.0261 07/31/2016 - - 08/01/2016 0.0091 0.03 08/02/2016 0.0093 0.03 08/03/2016 0.0209 0.03 08/04/2016 - - 08/05/2016 - - 04/30/2017 - 05/01/2017 0.0298 05/02/2017 0.0218 05/03/2017 0.0260 05/04/2017 - 05/05/2017 - 18 of 90

Date Towerbrom Floguard Spectrus BDl 500 Nalco Spectrus Floguard mg/L MS6237 mg/L 73551 CT1300 MS6236 TRC mg/L Quat mg/L mg/L mg/L Phosphate/Zinc EO/PO Quat Phosphate 07/23/2017 - - - 07/24/2017 0.0124 0.04/0.01 - 07/25/2017 0.0081 0.04/0.01 - 07/26/2017 0.0232 0.04/0.01 0.03 07/27/2017 0.0179 - - 07/28/2017 0.0296 - 0.03 5/13/2018 5/14/2018 5/15/2018 0.0229 0.030 0.0275 5/16/2018 0.0159 0.030 0.0275 5/17/2018 0.0133 0.030 0.0275 5/18/2018 10/07/2018 - - - - 10/08/2018 0.0221 - - 0.02852 10/09/2018 0.0098 - 0.0332 0.02852 10/ 10/2018 0.0187 0.031 0.0332 0.02852 10/11/2018 0.0200 0.031 - 0.02852 10/12/2018 0.0187 0.031 - 0.02852 04/28/2019 - - - -- 04/29/2019 0.0069 0.047 - - 04/30/2019 0.0109 0.047 - - 05/01/2019 0.0196 - - - 05/02/2019 0.0341 - - - 05/03/2019 0.0281 0.047 - 08/04/2016 - - - - 08/05/2019 0.0227 0.047 - 0.02852 08/06/2019 0.0071 - 0.0332 0.02852 08/07/2019 0.0117 0.047 0.0332 0.02852 08/08/2019 0.0142 - 0.0332 0.02852 08/09/2019 0.0078 0.047 - 0.02852 5/3/2020 - - - 5/4/2020 - - - 5/5/2020 - - - 5/6/2020 0.0045 0.049 0.0332 5/7/2020 0.0055 - 0.0332 5/8/2020 0.0183 0.049 0.0332 10 '4/2020 - - - 101512020 <0.05 0.046 0.02806 l Q/6/2020 <0.05 - 0.02814 10, 7/2020 <O.O: 0.046 0.02795 10/8 '2020 <0.05 - 0.02797 10 9/1020 <0.05 0.046 0.02795 19 of 90

Sequoyah Nuclear Plant Biomonitoring October 06 - 13, 2020 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets 20 of 90

BIOMONTTORING CHAIN OF CUSTODY RECORD Page _1_ of _I_ Clicm: TVA Environmental Testing Solution, lnc. Delivered By (Circle One): Project Nmne: Sequoyah NP Toxicity 3S1 Depot Street. Fed£:-. UPS Bus **c1 icnt_ _ P.O. Number: NIA Asheville, NC

  • 1Ot~specify): .5; *c. 11 28801 General Comments:

Facility Sampled: Sequoyah NP

                                                                                                                                                                                        , *.         ,, ---1 .,..... *.J Phone:               828-350-9364                                                 1;)*I I*.
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NPDES Number: TN0026450 .. . .r:lr--; : ,: I~') .l I *.. ,+I.:.. **:i:- \./\ '?.>~\~\(.. c;r"r *. :i "**. :..J __. Collected By; j*.'.-2::.--P- .- ., *~'IC gk-~

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Whole Effluent Sample Receipt log Page .JlJ;,

                                                                 *sample 1emperature performed using verlled Genera l Use Thermometer SN: _ _ _1_7_ (f1
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_____1..=oo(;).,__ Date Time Received Received *sample Project Sample Sample name and description State Comments Received Received by from Temp. (°C) number number 10-05-20 1327 J. Sumner TVA Courier 2.6 15419 201005 .01 TVA* Watts Bar Nuclear, Outfall 101 TN 10-05-20 1327 J. Sumner TVA Courier 2.5 15419 201005 .02 TVA

  • Watts Bar Nuclear, Outfall 101 - Intake TN 10-05-20 1327 J. Sumner TVA Courier 2.2 15419 201005 .03 TVA - Watts Bar Nuclear, Outfall 113 TN 10-05-20 1327 J. Sumner TVA Courier 3.0 15419 201005 .04 TVA
  • Watts Bar Nuclear, Outfall 113 - Intake TN 10-05-20 1420 J. Sumner TVA Courier 1.0 15420 201005 .05 TVA - Sequoyah Nuclear, Outfall 101 TN 10-05-20 1420 J. Sumner TVA Courier 2.0 15420 201005 .06 TVA- Sequoyah Nuclear, Outfall 101- Intake TN SOP G4 - Exhibit 64 .2, revision 01-03-12 22 of 90

BlOi\*IONITORlNG CHAIN OF CUSTODY RECORD t>aac I of t t:, - - - - Client: TV 1\ Environmental Testing Solution, Inc. Di!livcred 0y (Circle One): Project Name: Sc11uoy;ih NP Toxicity 351 Depot Street. FedEx Ul'S Bus Client P.O. Number: N/1\ Asheville, NC § (spec if}*) : Svn *c* General Commcn1s: JrilU.. 7li\~ES 1;;,q.s,1:./<J.1 Facilily Smupletl: Sequoyah NP 2880t I <l 1 ~ .,.\a\k (v~, ,':l' ,;n .J.$' {)t-1.. 1*)-*< - J->) NPDES Number: TN0026450 Phone: 828-3 50-9364 :r ,.r.-~1-<- . " " '? r§' (): ,;;:;;>

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23 of 90

Whole Effluent Sample Receipt Log Page ___uj

                                                                   *sam ple temp eratur e perform ed us,ng verflcd General Use Thermometer SN: _ _ _ _\_i~ a"\

_ 'S_'-\_l\_1.---"0_ Date Time Received Received "Sample Project Sample Sample name and description State Comments Received Received by from Temp. (0 C) number number 10-07-20 1010 K. Keenan UPS 2.6 15443 201007 .01 Camden County WTP NC 10-07-20 1014 K. Keenan Fed - Ex 3.6 15444 201007 .02 Belews Creek S5 - 006A NC 10-07-20 1014 K. Keenan Fed - Ex 1.1 15445 201007 .03 Franklinton WTP NC 10 20 1014 K, Keenan Fed - Ex 2.5 15446 201007 .04 Goldsboro WRF NC 10 20 1014 K. Keenan Fed - Ex 1.1 15447 201007 .05 HamletWWTP NC 10-07-20 1014 K. Keenan Fed- Ex 1.0 15448 201007 .06 IP - Rieglewood NC 10-07-20 1014 K. Keenan Fed - Ex 4.4 15449 201007 .07 MonroeWTP NC 10-07-20 1014 K. Keenan Fed - Ex 3.1 15450 201007 .08 Perdue, Inc. NC 10-07-20 1014 K. Keenan Fed - Ex 3.0 15451 201007 .09 PonzerWTP NC 10 20 1014 K. Keenan . Fed - Ex 1.4 15452 201007 . 10 Buck CTCC - 007 NC 10-07-20 1014 K. Keenan Fed- Ex 2.3 15453 201007 . 11 ALCOA - 005 NC 10 20 1014 K. Keenan Fed - Ex 2.3 15454 201007 . 12 ALCOA-013 NC 10-07-20 1014 K. Keenan Fed - Ex 2.1 15455 201007 . 13 PCS Phosphate, Inc. NC 10-07-20 1014 K. Keenan Fed* Ex 2.7 15456 201007 .14 Atlantic Beach WTP NC 10-07-20 1014 K. Keenan Fed - Ex 2.7 15457 201007 .15 DixanWTP NC 10-07-20 1014 K. Keenan Fed

  • Ex 1.7 15458 201007 .16 New Hanover County WTP NC 10-07-20 1014 K. Keenan Fed - Ex 2.3 15459 201007 .17 Washington WTP NC 10-07*20 1014 K.Keenan Fed* Ex 3.3 15450 201007 .1 8 Richland Township WTP NC 10-07-20 1014 K. Keenan Fed. Ex 2.2 15451 201007 . 19 RWS NC 10-07-20 1014 K. Keenan fed - Ex 2.2 15462 201007 .20 Skyco WTP NC 10-07-20 1014 K. Keenan Fed* Ex 2.2 15463 201007 .21 Stumpy Point WTP NC 10-07-20 1337 J. Sumner TVA Courier 1.1 15420 201007 .22 TVA- Sequoyah Nuclear, Outfall 101 TN 10-07-20 1337 J. Sumner TVA Courier 1.7 15420 201007 .23 TVA - Sequoyah Nuclear, Outfall 101 - Intake TN 10-07-20 1459 J. Sumner TVA Courier 1.8 15419 201007 .24 TVA - Watts Bar Nuclear, Outfall 101 TN 10-07-20 1459 J. Sumner TVA Courier 1.5 15419 201007 .25 TVA - Watts Bar Nuclear, Outfall 101 - In take TN 10-07-20 1459 J. Sumner TVA Courier 2.2 15419 201007 .26 TVA - Watts Bar Nuclear, Outfall 113 TN 10-07-20 1459 J. Sumner TVA Courier 2.0 15419 201007 .27 TVA - Watts Bar Nuclear, Outfall 113
  • Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12 24 of 90}}