ML20072U403
| ML20072U403 | |
| Person / Time | |
|---|---|
| Site: | McGuire, Mcguire  |
| Issue date: | 02/28/1991 |
| From: | Haller W DUKE POWER CO. |
| To: | NORTH CAROLINA, STATE OF |
| References | |
| NUDOCS 9104190128 | |
| Download: ML20072U403 (22) | |
Text
l Duke Iburr Company (70n373 40ll Nuclear Production Department
~=
- *f o Box 1007 Charlotte, NC 282011007 r,
iC j.g,
DUKEPOWER 1
AR' La <
March 27, 1991 Division of Environmental Management Water Quality Section Attention:
Central Files P.O.
Box 27687 Raleigh, NC 27611
Subject:
Duke Power Company NPDES Monitoring Report for McGuire Nuclear Station
- NC0024392 File:
MC-702.25
Dear Sir:
In accordance with Part II, C(2) of the above referenced NPDES permit, duplicate copies of the monthly monitoring report for February, 1991 are enclosed.
As reported to the Mooresville Regional Of fice of NCDEHNR on February 26, 1991, a visible foam was seen at the discharge of outfall 005. Since this occurence, efforts to identify the exact source have only identified a probable source of the foam. There was an increase in the amount of cleaning done at the vehicle maintenance f acility which may have lead to the increased discharge of foaming agents.
All individuals involved with cleaning activities at the McGuire site have since been advised of the importance of the proper use of cleaning agents and the impact improper usage has on the operation of the wastewater treatment stems.
-Piease direct any correspondence or questions concerning the McGuire Nuclear Station NPDES Program to M.
C.
Griggs (704) 373-7080, Nuclear Environmental Compliance.
Very truly yours, k,
W.'A.
Haller, Manager Nuclear Technical Services MTK/1478 Attachments 4
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E Fl.ljENT NPDES PERMIT NO:
NC0024392 DISCHARGE NO: 001 MONTH: February YE AR: 1991 FACILITY NAME: Duke Power ComDany + McGuire Nuclear Station CLASS: LLCOUNTY: *cklenbure OPERATOR IN RESPONSIBLE CHARGE (ORC): Mark E. Bridges GRADE' L CERTIFIED LABORATORY: Station tumet/Centcal tab to 249 c.A. Bynum cxtex stoex w one HAs cwANeto I-- PERSON (s) COLLECTING SAMPLES :
I GIM Mit Hl!IlW Mail engans and one copy to.
p
,,trei Files 11 MCuttit in0 (05Ptl11 TO ATT Cen gn yy ful N!! 0F 57 inoett0st x
(2
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1 2400-2.5 68.5 2
2400 2.5 65.7 3
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4 2400
?.5 69.2 0
5 2400 2.5 68,4 6
2400 2.5 68.4 7
2400 2.6 - 68.0 7
+.,
8 pann p,c 73.4 9 2400 2.5 71.2 10 2400 2.5 70.5 11 aann aa 3n n
+
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4 to P400 2,5 67.1 17 2400 2.5-66.0
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18 2400-2.5 66.4 8 2400 28; 67.6 a2 y
- j.
20 2400 2.5 57.7 21 2400 2.5 59.9
, ma 22 2400 2.5 6J.7 23 2400 2.5 65 M
~
24 2400 2.5 68.5 2d 2400 2.5 68.0 w
26 nnn
,e U ?dno pq rg 6 28 2400 2.5 67.1 at 30 l
31 A"'*9' 2.5 66.7 Max.
2.5 73.4 p
Min.
2.4 57.7 Como.tCl/ OmbiGl C
C M_onthly Limit g
'a s
tt R. l.1 t i l, A4 )
Facility Status: ( Please check one of the following)
All monthly averages and / or other limitation do meet permit monitoring requirements @
( Compliant)
All monthly averages and / or other limitation do not meet permit monitoring requirements l I
( Noncompliant)
If the facility is noncompilant, please comment on corrective actions being taken in respect to equipment, operation, maintenance, etc. and a time table for improvements to be made.
( Attach additional sheets if necessary) j I certify that this Report is accurate and complete to e t of my knowledge:
D hb Signature of Permittee PARAMETER CODES 00010 Temoeroture 00356 011 and Crease 00950 Dissolved fluottda 01077 tilver 39516 PCSS 00065 Stream Stage 00600 ' Total nitrogen 01002 Total Arsentt 01047 Total vanadium 39941 ILoundus 00076 Turtidity 00610 assonia Wittogen 01027 Cadatum 01092 ttac 50047 Mas. rtow dvetas 24-hr. pertod 00300 Dissolved 0061$ Total tjeldahl 01032 Nezavalent 0110$ Total Alumisus
$0044 Mia, flow duttat 24.hr, perte4 Orygen n1trosee Chtomium 00310 300 0(665 Total thosonotous 01034 Chroalum 01147 Total telasium 500$0 riow 5
00340 CCD 00720 Cyanide 01037 Total Cobalt 31504 Total Collf orn
$0060 Total testdual chlettae 00a00 pt 0074S Total su111de 01042 Copper 31614 Tecal Celtfets, 71440 Formaldehyde MP3, Tube 00500 Totsi solids 00127 Total Magnesium 01043 Total troo 31616 Tecal Celtfore 71900 Mercury 00130 TSS 00929 Total Sodtun 01051 Lead 3t730 Total thenolics 41314 Ferrocyaaldes 00545 settiaatte 00940 Total Chloride 01067 Nickel 38260 MBA3 43451 Time s tid.
The monthly average for fecal collform is to be reported as a geometric MEAN, if using alternate units for reporting data, please designate.
E
.{ {
NPDES PERMIT NO:
hc0024392 DISCHARGE NO:
002 MONTH:
bru"Y YE AR: 1991 Duk' P0"" co@any. McGuire Nuclear Station CLASS: 1!! COUNTY : "'ck1" dun FACILITY NAME:
M8'k E B"*$
GRADE: til OPERATOR IN RESPONSIBLE CHARGE (ORC):
Station Exemot/ central Lab 10 240 CERTIFIED LABORATORY:
- c. A. Bynum curex stock
- cac as emcro (- PERSON (s) COLLECTING SAMPLES :
I Mim lhil W$ f(NiI Ma.4 ongew and one copy to
!$ Attttill ARD touPttf( 10 Div e o Envir sa Managemn
[. /.
w p $ 74g7 fut 8131 Of at nWatttt[
jg
$lgneture of operator iri responsible therge Raee.gh North Cwonna 17att so ao 00400 150060100610 00530 006 ali!130665 s0630 39260' 00945 11051 WD M
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- GO Unit MG/L MG / t.
MG/L dG/L u G! t.
MG/L M c / t_
2
'3 4
0920 0.013 7.9
<0 f 05 5 0800 0.568 7.0 5.3 3.1 (0.1 0.12 8.29 0.1 '
3689
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9 10 1120 8
4 11 0010 0 (16 7.0 en nm 12 0810 0,147 f
ta 1200 7.3 7
a no,n n.,,
IS 0935 0.599 7.6 1e 0915 0.601 7.8 17 1030 0.124 18 18 0900 0.038 7.0 0.148 20 0820 0.579 7.1 I
21 0840 0.602 7.0 22 no,n n_nio 23 24 2a0910 0.018 7.2 0.060 26 0900 0.544 4.9 27 0835 0.595 7.6 to0845 0.591
~7.0 2d 30 31 Avera9*
0.406
<0.055 Max.
0.622 8.4 5.3 3.1 (0.1 0.148 0.12 8.29 0.1 3689-
<300 P
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Montney Limit D F \\1 Fo m Ni R 1 1 it! W i
4 Facility Status: ( Please check one of the following)
All monthly averages and / or other limitation do meet permit monitoring requirements @
( Compliant)
' All monthly averages and / or other limitation donot meet permit monitoring requirements l I
( Noncompliant)
If the facility is noncompilant, please comment on corrective actions being taken in respect to equipment, operation, maintenance, etc. and 1
a time table for improvements to be made.
( Attach additional sheets if necessary) l certify that this Report is accurate and com ete to t e bes of my knowledge:
1
.A r __
Signature of Permittee
. PARAMETER CODES
-00010..teocrature 00$$4. 011 and Crease 00950 01soolved Fluoride 01077 Staver 39516.Pcts 00061 Streas State 00600 Total uttrogea 01n02 total Arseate 01087 total venadium 39941 toundup 00076-Tutttdity 00610 Am onta Nitrogen
_01027 Cadstum 01092 Itac
$0047 '
Mas, flow durto:
24-ht. perncu 00100 Otseelred 00613 tota 1 K3eldahl 01032 Mezavalent 01105 total Alusieum 30044 Mia. flow.eurtas Ozygen -
N1trogee Chroatum 24*hr. Perto4 00310 800 00665 total rhostnorous 01034 Chroeim 01147 total saloale 50050 flow 5
00340 C00 00720 Cyanide 01037 total Cobalt 31504 total Califore 50040 total nacidual chlertae 00400 pg 0074$ total sulfide 01042 Cooper 31614 focal Caliform.
71440 ' Teresidehyde MPW, Tube.
00100 - Total Solids 00917 total Magnosta 01045 total trea 31616 fesel Calif ets 71900 hercury 00530 T33 00929 totsi sodte 01051 Lead 3t730 total Phenotice 61318 Terrocyanides 00$45 ' Settlaabte
~ 00940 total chlottde 01067. Nickel 38260 MBAS 83652 time solida The monthly average for fecal coliform is to be reported as a geometric MEAN.
If using alternate units for reporting data, please designate.
5 m
m
..- m.
m minummi iei us i ill lund u
E::LUENT NPD'ES PERMIT NO : Nc0024392 DISCHARGE NO: 003 MONTH:
' ' D "' "Y 1"1 YEAR: _
FACILITY NAME:
Duk' 'ow'r Company. M:Guire Nuclear Station CLASS: I COUNTY: "' klenbur9 OPERATOR IN RESPONSIBLE CHARGE (ORC): ""
- E ? "
1 GRADE:. !!!..
CERTIFIED LABORATORY: station tiempt/centesi ud to 248
' ^ B'""*
execx stoex
- one was cwascro p PERSON (s) COLLECTING SAMPLES:
IE II UII N Mait or9nal and one copy to 116CMM 440 (0WH M Divi o Envir ai Management fut N!! Of W1 teottt041 X
^-
>As p go 74gp Ra.e.gn North Cwt >.no 174i1 Signoture of operator in responsi' ole charge
$4450 00400 1 500601 003101 00530 31616 on m '
9e 1
5 I
b (([n'nftfie$ '
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0925 0.012 3
0915 0.007 4
0925 1 022 a.5 5
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0.22
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0845 0.029 7
0915 0.029 e
noen yn77 e
0915 0, cog 10 0935 0.007 tt 0530 0.007 b.4 I2 09?^
0.0?6 7
4 13 0820 0.036-14 0920 0.040 15 nonn nny IO 1006 0.007 17 1110 0.004 18 0900 0.007 1.4 8.no9c n n1a 7-7 9.17 79 n re n,a N OR40 0.029 21 0930 0.017 S w.
22 0845 0.029 M 1000 0.010
+
24 lits q,no7 as 0850 0.006 3.0 2e 0805 0.022 7.5 27 noen nnw 25 0900 0.029 2d 30 31 A*9' O.20 3.6 24.7 62.4
<?*
0.28 Max.
0.40 7.7 5.4 34.7 73.8
<2 0.34
<0.1 Min.
0.004 7.4 1.4 14.7 50.9
<2 0,22 Como (C)/ Orob!G1 n
n a
n r-n n
Monthly Limit Di'1 Fo*r st R. l,;l-54i
i Faciilty Status: ( Please check one of the following)
All monthly averages and / or other limitation do meet permit monitoring requirements @
( Compliant)
All monthly averages and / or other limitation donot meet permit monitoring requirements l l
( Noncompliant)
If the facility is noncompliant, please comment on corrective actions being taken 1.. respect to equipment, operation, maintenance, etc. and a time table for improvements to be made.
( Attach additional sheets if necessary) l certify that this Report is accurate and complete to the best 6my knowledge:
hoYJ Signature of Permittee PARAMETER CODES 00010 Temperature 00$36 011 and Crease 00910 Diseelved riuaride 01077 $119er 39316 PCSS 00043 Stream Stage 00600 total nitroges 01002 Total Arsente 01087 Total Vanadtun 39941 Rouadup 00074 Tvrtidity 00410 amnesia Nitrosen 01027 Cadatum 01092 Itaa 50047 Man. flow durtos 24-hr. pertoe 00300 Dissolved 00613 total tjeldahl 01032 heuvaient 01105 total Aluminun
$0044 Mia. flow duria Oryges Nitroges Chromium 24*hr. Perted 00310 500 00643 Total Fhesphoroue 01034 Chromiwa 01147 Total seleatum 50050 Flav 3
C0340 CCD 00720 Cyaalde 01037 total cetalt 31504 Total Colf fore 50050 total teatdual Chlertae 00400 pe 00745 Total sulfide 01042 Co,per 31614 fecal Califers.
21880 Fernaldahrde MPW, Tube 00$00 total Solida 00927 Total Magnesium 01045 Total tron 31616 Fecal Colliers 71900 hercury 00330 TSS 00929 total Sodium 01051 Lead 3F730 total thenoltas 81314 Ferrocyanidas 00545 Settlaanle 00960 Total Chler14e 01067 NLakal 34260 MRAS 65652 Time se11de The monthly average for fecal coliform is to be reported as a geometric MEAN.
If using alternate units for reporting data, please designate.
E:F.UENT NPDES PERMIT NO:
Nex24392 DISCHARGE NO: 004 MONTH:
'abruarv YE AR: 1991 Ouke Power Company. McGuire Nucle v Stettee CLASS: i t COUNTY: Mecklerburn FACILITY NAME: _
"* '" !
- 8 ' " " 5 GRADE:- !!!
OPERATOR IN RESPONSIBLE CHARGE (ORC):
CERTIFIED LABORATORY:
statica t umot/centrai tad to 248
'ichard "eker curex stocx
- one was cumcro f-PERSON (s) COLLECTING SAMPLES:
I EIIN I"II IUIIISII Mail engnad and one copy to.
II IIII I"O II I I II O mon o er e Management IW! Sill Of 51 tt0WL(DGI X
C/ "
p 74g7 RWm North Carosene 17611 Signcture of operator in responsible charge 50 lit
??530 1005561 61313 I
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>F-7 2400 0.006 8
e 10 11 12 la u
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16 17
'r 18 in 20 21 2400 0.011 p~.
22 2J 24 N
26 v,
2e 2400 0.006 M
30 m
Average 0.007 i
Max.
0.011 Min-0.00s i
Como.tCli OrobiO)
Monthly Limit
'~~
L Facility Status: ( Please check one of the following)
.All monthly averages and / or other limitation do meet permit monitoring requirements -
( Compilant)
. All monthly averages and / or other limitation donot meet permit monitoring requirements l l:
( Noncompliant) if the facility is noncompilant, please comment on corrective actions being taken in respect to equipment, operation, maintenance, etc. and a titre table for improvements to be made.
( Attach additional sheets if necessary).
e
'l certify that this Report is accurate and complete to th best my knowledge:.
h/
l Signature of Permittee ~
~
- 1
, j PARAMETER CODES
- 00010 --- Temperature J
.00$$6=~011 and Grease 00950 Dissolved fluoride
- 01077 $11Mr 39$16 - PCas 00065 8tream 8tese
- 00600.' Total utgregen 01002 Total Ateests -
01047 total Venedt e 39941= :3eundup,
cf 00076: Turtidtry.-
-00610 i meseta uttregen -
01027 : Cadaim.
01092 11ee
$0047 Man.~ flew dutiesJ 24 hr. perted '-
00300 Dissolved ~
00623 Total Ejeldahl.
01032. nemoveleet -
~ 01103 Total Almanum._
$0044 Iltia. flow duringj i
l
~
- Ouygen uttregen Chromia 24.hr. per1 4
,00310 -: 4c0
- 0064$ " Total theephereue.
-01034 Cheeste -
01147-Tesal Selante 500$4 Flow 3
l:
' 00340. < C00
= 00720. Crealde 01037 Total Cotelt 31304 Total Celtietu -
30060 Total teendual p
Chlevies C
l00400 'p3 00745 Total Sulfide 0104% copper 31614 Feasi Celtfeen.;
71000 femeldehyde =
. IWW. Tube-100$00 -? Total Solide :
. 00927 E Tesal Magnost m =
01045 ' Total tree 31616 Feasl Califers 71900 ~ Itateury g
00530=;TSS 00929 total sedte -
01031, t.ead -
if730 Total Phomeitte 01318.Ferrecyeegdeo g-
- 00$45 ' Settlechte 00940 ' Tete! Chloride
'01067 Nickel 3:260 leas 4$432 ' Time p
- S.lide-Ll The monthly average for fecal coliform is to be reported as a geometric MEAN.
<lf using alternate units for reporting data, please designate.
M
=
f w
n-r--
v w
r-
~+
.m.
NC0024392 DISCHARGE NO: 005 MONTH:
February YEAR: 1991 FACILITY NAME: Duke power compan<. Mccuire Nuclear statica CLASS: ?! COUNTY:
- cklendure OPERATOR !N RESPONSIBLE CHARGE (ORC):
M8'" E Br'de'5 GRADE: - !!!
CERTIFIED LABORATORY:
Station Enemot/ Central Lab 10 248 PERSON (s) COLLECTING SAMPLES:
C A BY"'m ewecx uock
- oac Mas cwAscro p
' EIE I"AI Id #II Mel ongsnal and w copy se II OIIII III UU N
oh as Management b
p$ y[gy fWI N51 of et Enonttitt x
e Raieign North Caroww 176t)
Signeture of Operator in fesponsible charge 400st 00400 I 00310 00610 ?0c30 1!'16 00EE6' 0^4!0' '06 ' On'?'
o^' M
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3m 3 2E EEE 36 at 63 m
m mes uso unit MGet MGet MGet flooMt MG/L MG/t MG/t MG/t MG/t VG/L UG'l P-8 i
e 3,34 w:
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0845 0.687
'J 0905--
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4 0920 0.570 0
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- n -9 41M 9an 6
091n 0.976 7
0900 1.014 e
0615 0.696 9
sqn n _97 10 0925 0.572 n
0810 0.724 12 0610 0.572 4
0815 0.?95 54 0900 0.690 is 0835 0.410 to 0915 0.787 iT inin 0 oo r, 18 0850 0.510' le 0900 0.409 10.0 5.1 11.0
<2
<0.1 20 0620 0.409 21 onan
-o,aps c m..
22 0830 0.232 21 1100 0.414f 24 110b 0.w M 0910 0.?00 e*
26 0800 0.200 as 0836 0,117 28 no3e n,,,
5
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30 31 g-Average g gg,
,n Max.
1.014 10.0 S.1 0.05 11.0, <?
<0.1 14.18 0.09 0.8 0.'c
<100 200 Min.
0.117 8.1
<0.1 Como iCli ornbiG)
G G
G G
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-G G
Monthly Limit DENi Form NiR 1 1 i ll da i
N Facility Status: ( Please check one of the following)
All monthly averages and / or other limitation do meet permit monitoring requirements M
( Compliant)
All monthly averages and / or other limitation do not meet permit monitoring requirements l l
( Noncompliant)
If the facility is noncompilant, please comment on corrective actions being taken in respect to equipment, operation, maintenance, etc, and a time table for improvements to be made.
( Attach additional sheets if necessary)
I certify that this Report is accurate and com lete to th best f my knowledge:
Signature of Permittee PARAMETER CODES 00010 femperature 00556 ott med Creese 00950 Dissolved T1uoride 01077 stiver 19$16 PCas 00065 Stream stage 00600 total uttroges 01001 Total Areesta 01087 total vesadium 39941
- soundu, 00076 tutsidity 00610 ammmonia uttregen 01027 Cadatum 01092 Itac 30047 Man. flow duttag 1
24.ht. period 00300 Otseelved 00613 total Kjeleatl 01032 Henavaient 01103 fetal Alusieue S0044 nta, flow duttas i
oryges attrogen Chroalus 24.ht. pettod 00310 400 00665 fetal rhosphoroue 01034 Chromiun 01147 total Seleatus
$0050 - Flow 3
00340 CCD 00720 Cyaalde 01037 total cobalt 31304 tetel ce11fors
$0060 Total testdual Chlottne 00400 pt 0074$ total sulfide 01042 tw 31414. fecal Celtterg.
71480 Formaldehyde Mrs, tube 00$00 total seitda 00127 total Magneatus 0104)
Total Ira 31616 Focal Celtiera 71900 Mercury 00$30 ~ ts:
00129 total sedtwa 01031 tsad 3t730 total themaltas 81318 Ferree7entdes 00$45 settloable 00940 Total Chlottie 01067 Michet 38260 MRA.1 05612 ftse settde The monthly average for fecal collform is to be reported as a geometric MEAN.
If using alternate units for reporting data, please designate.
E Fl.llENT NPDES P5RMIT NO:
NC0024392 DISCHARGE NO:
006 MONTH:
'
- h m "v
. YEAR: ult Duke Power Company. McGuire Nuclear Station CLASS: l.L. COUNTY: "eek1rsere FACILITY NAME: _
OPERATOR IN RESPONSIBLE CHARGE (ORC):
M8'k E 8'He'5 GRADE: m CERTIFIED LABORATORY: station Exemot/ Central tab to 248 CHECK BLOCK IF OAC MAS CHANCED 1 Cittin in1T full 11798f Mad or9na and one copy to-h
',[g*n y ymp 13 4CCalift He t0gMf! 70 pf[7,gy fut 0131 Cf et 008ttH!
X
/
/
Raee.gh North Crohna 17411 II9RO'Vr# Of Operetof iff resp 068ible therge Sotst 004 00 1 010421 01045 I
k I Hn
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att att unit UG/L UG/L i
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6 7
- q. ;
a 9
-s.
10 100 $W1.IIH DONE ' HIS PE L100 11 12 13 r
14
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=
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20 21 y-L.
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26 d
2e in ix.
3C 31
+
Average Max.
Min.
Como.iC)/ OrnblG)
Monthly Limit DEM Form MR.I.! ill Sa n
Facility Status: ( Please check one of the following)
All monthly averages and / or other limitation do meet permit monitoring requirements @
( Compilant)
All monthly averages and / or other limitation donot meet permit monitoring requirements l l
( Noncompilant) if the facility is noncompliant, please comment on corrective actions being taken in respect to equipment, operation, maintenance, etc. and a time table for improvements to be made.
( Attach additional sheets if necessary)
~
l certify that this Report is accurate and complete to t bey of my knowledge:
NY.==y=.
Signature of Permittee w.
PARAMETER CODES 00010 Temperature 00$$4 011 and Crease 00950 Dissolved Fluoride 01071 5119er 39514 Pcts 00065 Strome Stege 00600 Total uitrosee 01002 Total Arsenic 01047 Total Yamadium 39941 Roundue 00076 Tuttidity 00610 ammonia Ritresee 01027 Cadatum 01012 Zine
$0041 Man. fisw duttas 24 hr. perted
[
00300 Dissolved 00623 Total Kjeldahl 01032 kazavalent 01103 Total A1uateus 50044 Ria. flow outtas !
Orfgoe uttrogee Chromium 26.ht. pertoo 00310 800 00643 Total thee,nereus 01034 Chromium 01147 Total selsatum 50050 Flow 5
00340 COD 00720 Cyanide 01031 Total cobalt 31504 Total cellform
$0060 Total tesidust Chieriae 00 00 95 0074$ total sulfide 01042 Cooper 31614 Tecal Caliform.
71890 formaldehrde nru, ta.
00500 Total Se11de 00927 Total Magnesium 0104$ Total tree 31616 Tesal C411fers 71900 hertury 00$30 T18 00929 Total Sodium 01031 Lead 3t130 Total these114e 31318 Ferroeveeldes 00$45 Settleable 00940 Total Chloride 01047 Nicksi 38260 MBAS 45452 Time solide The monthly average for fecal collform is to be reported as a geometric MEAN.
If using alternate units for reporting data, please designate.
MN0291D1 EfIluent Aquatic Toxicity Report Forrn Acute Pass / Fall Date 2/6/91 Factity McGuire Nuc1 ear 5tation NPDES 4Nc0024392 Ptpe MCounty Meck1enburo laboratory Performing TesQuke Power Prod Env. Serv.
Commentssacole TemDerature uoon receiDt was 2.6'C.
Two aninals drie:
0"
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S(pture of Operator in Responsible Charge Environmental Sciences Branch V A _07 G \\ A_ -O:
0- c, en-nmen,-no0emem, N.C. Dept. of EHNR P O, Box 27687 North Carolina Acute Pass / Fall Bloassay Rotegn. Nonn Carohno 27611 Collection Date: 2/5/91 Organism Tested Collection Time: 0915 Daphnia culex Test Start Date: 2/5/91 Control
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samnteTvre / Duraum 7.9 7.8 pH Grnb comn Durnttor Treatment 7.3 7.5 X
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Hardness (mg/l) 42 5 Control R.'
O.0 Spec.Cond.(gmhos) jag ga o,o, Wemem g-9,9 Chlorine (mg/l) W y,y, Mortality Replicate Mean Mortality Treatment 1 (Control)
A B
C D
0 0
0 0
0 Treatment 2 (Exposurel A
B C
D 0 'o Concentration 4
/o
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Tested 90 0
20 0
0 5
(NOTE: If mean control mortality e.xceeds 10%. the test is considered invaltd)
Calculate using Calculated Student's t Arc Sine
-1.00 PASS
.x Square Root Tabular Student's t transformed data (ONE TAILED)
-3.14 pg If the absolute value of the calculated t is less than or equal to the absolute value of the tabular t, check PASS.
If the absolute value of the calculated t is greater than the absolute value of the tabular t, check FAIL.
If all vessels within each treatment have the same response but the treatment two response is greater than the control.
check FAIL.
DEM form AT 2 (10/90)
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bNO291D2 Effluent Tcxicity Report Form Chronic Pass / Fall and Acute LC50 Date 2/8/91 M McGuire Nuclear Station NPDESNc oo?4W Bpe QRCounty wi ec Duke Power Prod.Env. Serv.
Comments Samole locoed in and re-Laboratory Performing Test fricerated within 28 minutes of col-d 2. /M lection.(Sample iced at time of col-X 1eetion ) nocomant =tinn att.%
Sl[rydtture of Operator in Responsible Charge Environmental Sciences Branen V A _ O,T G \\ A
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Div of EnvironmentalManagement N.C. Dept. of EHNR P. O. Box 27687 North Casclina Ceriodachnla Chrortie Pass /Fa!! Reoroduction Bloassar Roleigh. North Coroino 27611 CONTROL ORGANISMS 1
2 3
4 5
6 7
8 9
10 11 12 Chronic Test Rttuhl
- Young Produced Calculated t
% Mortality Avg.Reprod.
Adult (L)tve (D )ead Control Control Ef!!uent%
1Yeatment 2 Treatment 2 TREATMEt(T 2 ORGANISMS 1 2
3 4
5 6
7 8
9 10 11 12
% control rganisms PASS Fall
- Young Produced producing 3rti brood Adult (L)1ve (D lead Check One i
Comotete This For Elther Test lest Start Uste 1st sample.
1st sample 2nd cample 2 / 6 /01 Control Collection (Start) Date pH Sample 1 2 / 5 /91 Sample 2 NA /
/
Treatment 2 Samole Tvne/ Duration N
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y Sample 1 X
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t t
t 3
Sample 2 gg 1st sample 1st sample 2nd sample Control Hardness (mg/l) 43,4 g 4,Qg Spec.Cond.( mhost 149 512 h
t2 Chlorinelmg/l)
NM W/
e MhTest Sample temp. at receipt 10'9C (Mortality expressed as %, combining replicates)
A Concentration 0
6.25 12.5 25 50 100 w
Note: Please Mortality 0
0 0
0 5
0 Complete lhls Section Also Method of Determination,z
, L C 5 0 = _N). _ _ _ _ _ _ %
Moving Average O Probit O sta,tjene
,tortfcnu 9g Confidence Limits Other ________.,
Speannan Karber Control 8.5 9.1 7.7 7.8 High Organism Tested Daohnia Dulex 7.1 7.9 Conc.
9.6 0.5 I
DEM form AT 1 (3/87) rev.10/90
STATISTICAL ANALYSES The Ceriodachnia chronic toncity test measures the chronic toxicity of whole efnuents through both mortality and reproduction. Staustically significant toxic
. responses are to be de:ected using a t test (EPA /600/4 89/001, pg. 240) to compare mean reproduction in the efnuent concentration and the control. As described in EPA chronic toxicity testing protocol (EPA /600/4 89/001) mean reproduction is calculated by summing the total number of young produced per female until either the ume of death or the end of the experiment and dividing by the initial number of females exposed. An analysis of variance (ANOVA) provides an estimate of the pooled variance which is incorporated in the calculation of a t statistic. Based on a comparison of the calculated t value with the tabled critical value for a one sided comparison at a 0.01 confidence level, effluent chronic toxicity is determined to be either a PASS or a FAIL. In the case where there is only one treatment to be compared with the control, this t statistic is comparable to the Student t staustic for comparison of means from independent random samples. The t value is to be reported with test results.
The LC50 (acute toxicity section) represents the expected concentration of effluent that is lethal to 50% of the test organisms within the test period. A statistical estimation method must be used to obtain an estimate of the LC50 from concentration / mortality data. Uncertainty is quantifled through confidence intervals expressing the range of values within which the "true" LC50 could occur.
EPA acute toxicity testing protocols (EPA /600/4-85/013) detail several methods for estimating the LC50 and confidence intervals including: probit
~
analysis, logit analysis, the Litchfielo-Wilcoxon method, the moving average angle method, and the trimmed Spearman-Karber method. The recommended method is the trimmed Spearman Karber method because it is both model free,
and robust (i.e., not sensitive to anomalous responses), however, any of the above methods is acceptable. Confidence limits are an essential part of LC50 estimation and are to be included in reported toxicity test data.
310 ASSAY S W LOG T1g LOS te.
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