ML021070745
| ML021070745 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 04/11/2002 |
| From: | Nida D Tennessee Valley Authority |
| To: | Haskin E Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML021070745 (107) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 April 11, 2002 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control 401 Church Street, 6th Floor Annex Nashville, Tennessee 37243-1534 Attention: Ms. Evelyn Haskin, Enforcement & Compliance
Dear Ms. Haskin:
TENNESSEE VALLEY AUTHORITY - DISCHARGE MONITORING REPORT FOR SEQUOYAH NUCLEAR.PLANT Please find enclosed the Discharge Monitoring Report (DMR) of March 2002 for Sequoyah. Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Diedre B. Nida Environmental Supervisor Signatory Authority for Richard T. Purcell Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
>1 Pnnled on recycled paper
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved.
MAJOR ae.._ TV__A - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No. 2040-0004 ALdress_.L X200 TN0026450 1 1016 F - FINAL
.SQ_ Y.D i
. -__AISY - _T-LN 7314- PERMIT NUMBER DISCHARGE NUMB DIFFUSER DISCHARGE F. czilit__ . A -SEQU_.YAH NU__LEAPt ANT .
Location HAMILTON COUNTY EFFLUENT YFAr* I MQ I DAY ORIN G PT RIO DMO YEARAI* I nAY F oNmT -]
- From 02103101 1 To[02 103 131 NO DISCHARGE ATTN: Diedre B. Nida NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS FEMPERATURE, WATER DEG. SAMPLE ******** ** ******** ******** 15.6 m041 0 31/31 MODELD CENTIGRADE MESUEEouivINTI I I I I 00010 Z 0 0 DEG. C.
INSTREAM MONITORING TEMPERATURE, WATER DEG. SAMPLE 28.5 0 31 / 31 RCORDR CENTIGRADE MEASUREMENT I ***** (04)
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EFFLUENT GROSS VALUE
- 1 **4.-. .......... 4 __________
PH MEASUREMENT 7.6 7.8 (12) 0 I 8/31 00400 1 0 0 SU EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED *et 0
MEASUREMENT - IZ (19) 1 U I "1i " o 00530 1 0 0 MGIL EFFLUENT GROSS VALUE OIL AND GREASE I ******** <5 I <5 (19) 4/0 4/31 GRAB 00556 1 0 0 MG/L EFFLUENT GROSS VALUE FLOW, INCONDUIT OR THRU TREATMENT PLANT (03)
I ---- I ---^I .... 1/0 161/61 lmuuur¶L 50050 1 0 0 MGD EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL SAMPLt 50060 1 0 0 MEASUREMENT I - ******** <0.006 0.014 (19)
MGIL EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified Richard T. Purcell personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference a// attachments here No closed mode operation. The following injections occured: 1. PCL-222 (max. calc. conc. was 0.024mg/L--limit 0.100rmg/L) 2. CL-363 (max. calc. conc. was 0.009mg/L--limit 0.100mg/L) 3. PCL-222/PCL-401 (max. calc. conc. was 0.025mg/L--limit 0.100mg/L) may bebe used Page 1 of 2 EPA Iorm EPA su-1 INEV Form 3320-1 3I)
(REV 3/99) Previous edirions Previous editions may used Page 1 of 2
PERMITTEE NAME/ADDRESS (Include FacilitvName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 AA..__des __.-OX.200 (SUBR 01)
- _ _INTROC2FFIC. _.-_L) _
__-aO___YD -AIS)y_ -__T.738.__ PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Faqljrt__/ iA -SEQUOYLArUCLEPLAT Location HAMILTON COUNTY MONITORING PERIO __ EFFLUENT A1rN: Diedre B. Nida From YFAR 02 103 Mo 101 1 I To 102 103I 131AY 1 YEARI MO NO DISCHARGE = ***
NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIM TEMPERATURE - C, RATE OF SAMPLE ******** ******** ******** **** 0.3 CHANGE MEASUREMENT 32234 1 0 0 EFFLUENT GROSS VALUE TEMP. DIFF. BETWEEN SAMP. & SAMPLE t*
LJPSTRM DEG.C MEASUREMENT I ** I (0 4) 1 U 1 I0/ 0 1.f L
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EFFLUENT GROSS VALUE MEASUREMENT I I MEASUREMENT I I I I I I I I I I II BORON, TOTAL
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<U.Z I <U.2 (19) 10 1 /3 01022 1 0 0 MG/L EFFLUENT GROSS VALUE SAMPLE MEASUREMENT I I I II "NAME/TITLEPRINCIPAL EXECUTIVE OFFICER Idirection Certify under penalty ofinlaw or supervision thatthis document accordance and all with a system attachments designed werethat to assure prepared under my qualified TELEPHONE DATE Richard T. Purcell personnel properly gather and evaluate the information submitted. Based on my inquiry of the L) person or persons who manage the system, or those persons directly responsible for gathering Environmental Supervisor Site Vice President the information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 02 04 11 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO OFCRO UHRZDAET AEj NME ER M DAY A
-CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here CCW data for March 2002 is attached.
EPA porm 3320-1 (REV 3/69) Previous editions may be used Page 2 of 2
CCW Data for March 2002 CCW CHANNEL DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mg/L)
March 13, 2002 < 0.5 CCW TRENCH DISCHARGE DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mq/L)
March 13, 2002 1.0 Page 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Narae TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No. 2040-0004 Ad1dreos ... 0 .B.O_. ..
.(INTER._QF._FI CC..E.F.3 ]_
_DD-_DAISy. - - -.N.37384- TN645 101T F-FINAL PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE FaAcIjty_i_VNA -_EUQYAl-L_ L___PLANT Location HAMILTON COUNTY *MONITORING PFRIQQ EFFLUENT ATfN: Dledre B. Nida From 102101101 Fo YFARmMO 1 To1 02 MO AY I oYAR 03 I1311Q~y NO DISCHARGE Liii NOTE: Read instructions before completing this form.
NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS f
0 1/90 COMPOS i!!"
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I I I I I I I I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law thatthis document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified
(* I / ) TELEPHONE DATE Richard T. Purcell personnel properly gather and evaluate the information submitted. Based on my inquiry of the / -.. '"-.
person or persons who manage the system, or those persons directly responsible for gathering Environmental Supervisor Site Vice President the information, the information submitted is, to the best of my knowledge and belief, true, EnvironmentalSupervisor _ 423 843-6700 02 04 11 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED_______________
OR PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER TYPED OR PRINTEDOFIEORATOIEAEN ARA NME YAR M DY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here Toxicity was sampled from February 24, 2002 through March 1, 2002. Report is attached.
EPA Form 3310-1 'REV 3199 I e u sIUUO mliay L-SIlIUIa UseU Page I of 1
April 3, 2002 Diedre B. Nida, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, FEBRUARY-MARCH, 2002 Attached are two copies of the subject report for submission to the state of Tennessee.
The report provides results of compliance testing using fathead minnows. A copy of the report is included for your records.
Outfall 101 samples collected February 24-March 1, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent.
Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results indicate that fish pathogen interference is likely the cause of historic problems with WET tests for this effluent.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB 1B-M sqn february 2002m-jm
.1**L TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION/EXECUTIVE
SUMMARY
- 1) Facility/Discharger: Sequoyah Nuclear Plant/TVA Report Date: April 3, 2002
- 4) Type of Facility: Nuclear-fueled electric generating plant
- 5) Design Flow (MGD): 3,266
- 7) IQ10:2,992.4
- 8) Outfall Tested: 101 9) Dates Sampled: February 24- March 1, 2002
- 10) Flow on day(s) sampled (MGD): 1624, 1614, 1569
- 11) Pertinent site conditions: No unusual conditions reported. *
- 12) Test Dates: February 26-March 5, 2002
- 13) Test Type: Short-term Chronic-definitive
- 14) Test Species: Fathead Minnows (Pimephalespromelas)
Daphnids (Ceriodaphniadubia)
- 15) Concentrations Tested (%): Outfall 101: 10.98, 22, 43.9, 72, 100 Intake: 100 Pimephalespromelas:UV treated Outfall 101: 10.98, 22, 43.9, 72, 100 UV treated Intake: 100
- 16) Permit Limit Endpoint (%): Outfall 101 IC,>5 = 43.9
- 17) Test Results (%): Outfall 101: Pimephalespromelas:IC 2_Q 5 > 100 Ceriodaphniadubia: IC25 > 100 UV treated Outfall 101: Pimephalespromelas: ICQ 2 5 > 100
- 18) Facility
Contact:
Diedre B. Nida 19) Phone #: (423) 843-6700
- 20) Consultant/Testing Lab Name: Environmental Testing Solutions, LLC
- 21) Lab
Contact:
Jim Sumner 22) Phone #: (828) 862-8193 TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- Production/operation data will be provided upon request.
Notes: Outfall 101 samples collected February 24-March 1, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms. These results indicate that fish pathogen interference is likely the cause of historic problems with WET tests for this effluent.
METHODS
SUMMARY
Samples
- 1) Sampling Point: Outfall 101
- 2) Sample Type: Composite
- 3) Sample Information:
Sample Date/Time Date/Time Arrival Initial ID Date/Time Collected Received Temp. TRC* Used (MM-DD-YY/Time) (MM-DD-YY/Time) (0C) (mg/L) 11 (MM-DD-YY/Time) 02-24-02/1454 & 1518 02-26-02/1 105 0.9 <0.10 02-26-02/1522 02-25-02/0354 & 1418 02-27-02/1514 Intake 02-24-02/1539 02-26-02/1105 0.6 <0.10 02-26-02/1522 02-25-02/1439 02-27-02/1514 101 02-26-02/1026 02-28-02/1045 0.2 <0.10 02-28-02/1516 Intake 02-27-02/0926 02-26-02/1038 03-01-02/1500 02-28-02/1045 0.7 <0.!10 02-28-02/1516 02-27-02/0938 03-01-02/1500 101 02-28-02/1335 03-02-02/1058 0.6 <0.10 03-02-02/1506 03-01-02/1235 03-03-02/1453 Intake 1 02-28-02/1405 03-02-02/1058 03-04-02/1456 1.1 <0. 10 03-02-02/1506 03-01-02/1305 03-03-02/1453 03-04-02/1456
- Total residual chlorine.
"Collected in two 2.5-gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4) Sample manipulation:
Routine samples from Outfall 101 and intake were warmed to test temperature (25.0 +/-1 .00 C) in a warm water bath.
Aliquots of Outfall 101 and intake samples used for the side by side UV treated test were warmed to 24°C in a warm water bath and held in a 40Watt Rainbow Lifeguard UV Sterilizer for 2 minutes, prior to exposure of test organisms.
2
Test Organisms Pimephalespromelas Ceriodaphniadubia
- 1) Source: Aquatic BioSystems, Inc. In-house culture
- 2) Age: 23 hours2.662037e-4 days <br />0.00639 hours <br />3.80291e-5 weeks <br />8.7515e-6 months <br /> < 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> Test Method Summary (See Appendix A for additional information)
Pimephalespromelas Ceriodaphniadubia
- 1) Test Conditions Static, renewal Static, renewal
- 2) Test Duration 7 days Until at least 60% of control females have 3 broods
- 3) Dilution/Control Moderately Hard Moderately Hard Water Synthetic Water Synthetic Water
- 4) Number Replicates 4 10
- 5) Animals per Replicate 10 1
- 6) Test Initiation: (Date/Time)
Outfall 101 02/26/02-1438 EST 02/26/02-1522 EST UV Treated Outfall 101 02/26/02-1438 EST
- 7) Test Termination: (Date/Time)
Outfall 101 03/05/02-1430 EST UV Treated Outfall 101 03/05/02-1430 EST 03/05/02-1520 EST
- 8) Test Temperature: Outfall 101 Mean =24.6 'C Mean = 24.6°C (24.1-25.2°C) (24.2-25.3-C)
Test Temperature: UV-Treated Outfall 101 Mean = 24.70 C (24.2-25.5 0 C)
- 9) Physical/Chemical Measurements: Hardness, alkalinity, total residual chlorine, and conductivity were measured at the laboratory in each 100 percent sample.
Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10) Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
3
TOXICITY TEST RESULTS (See Appendix C for Bench Sheets)
- 1) Results of a Pimephales _promelas Chronic7-d Tox icity Test (Genus) (Species) (Type/Duration)
Conducted 02/26/02 03/05/02 Using Effluent From Outfall 101 (mm/dd/yy) (mm/dd/yy) (number)
Percent Surviving Test Solutions (time intervals used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% Effluent 100 100 100 100 95 92 87 22% Effluent 100 100 100 100 100 100 100 43.9% Effluent 100 100 100 100 100 100 100 72% Effluent 100 100 100 100 100 100 98 100% Effluent 100 100 100 100 95 95 93 Intake 100 100 100 100 100 100 100 IC 25 Mean Dry Weight (mg)
Test Solutions (Replication) 1 2 3 4 j Mean Control 0.702 0.646 0.618 0.494 0.615 10.98% Effluent 0.555 0.597 0.611 0.330 0.523 22% Effluent 0.580 0.551 0.522 0.535 0.547 43.9% Effluent 0.571 0.530 0.626 0.543 0.568 72% Effluent 0.579 0.619 0.693 0.560 0.613 100% Effluent 0.685 0.626 0.652 0.387 0.587 Intake 0.537 0.682 0.525 0.591 0.583 IC25 Value: > 100% Calculated TU Estimates: t < 1.OTUc 95% Confidence Limits: Permit Limit: 1.OTUc Upper Limit = N/A Lower Limit = N/A tTUc = 100/IC25 4
TOXICITY TEST RESULTS (continued)
- 2) Results of a Ceriodaphnia dubia Chronic 3-brood Toxicity Test (Genus) (Species) (Type/Duration)
Conducted 02/26/02 03/05/02 Using Effluent From Outfall 101.
(mm/dd/yy) (mm/dd/yy)
Percent Surviving Test Solutions (time intervals used - days) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 6.25% Effluent 100 100 100 100 100 100 100 12.5% Effluent 100 100 100 100 100 100 100 25% Effluent 100 100 100 100 100 100 100 50% Effluent 100 100 100 100 100 100 100 100% Effluent 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Data Test Solutions Replicates 1 2 3 4 5 6 7 8 9 10 Mean Control 22 25 26 23 24 22 26 21 20 25 23.4 10.98% Effluent 21 22 23 23 23 24 22 23 28 24 23.3 22% Effluent 26 24 24 23 24 21 25 25 23 27 24.2 43.9% Effluent 28 23 26 26 29 22 24 22 28 27 25.5 72% Effluent 26 30 26 30 26 28 27 30 31 28 28.2 100% Effluent 31 32 27 29 28 31 30 29 27 28 29.2 Intake 26 27 28 27 30 29 31 23 31 30 28.2 IC25 Value: > 100% Calculated TU Estimates: t < 1.0TUcc 95% Confidence Limits: Permit Limit: I.0TUc Upper Limit = N/A Lower Limit = N/A t TUc = 100/IC 25 REFERENCE TOXICANT TEST RESULTS (See Appendixes A and D)
Species Date Time I Duration I Toxicant Results (IC 25)
Pimephalespromelas 02/26/02 143 8 7-days KCI 561.38 mg/L(IC 25)
Ceriodaphniadubia 02/05/02 1337 7-days NaCI 1072.5 mg/L (IC 25 )
5
TOXICITY TEST RESULTS UV-TREATED (See Appendix C for Bench Sheets) 1I) Results of a Pimephales promelas Chronic7-d Toxicity Test (Genus) (Species) (Type/Duration)
Conducted 02/26/02 03/05/02 Using Effluent From Outfall 101, UV treated.
(mm/dd/yy) (mm/dd/yy) (number)
Percent Surviving Test Solutions (time intervals used - days)
_ 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% Effluent 100 100 100 100 100 100 100 22% Effluent 100 100 100 100 98 98 98 43.9% Effluent 100 100 100 100 100 100 100 72% Effluent 100 100 100 100 100 100 1o0 100% Effluent 100 100 100 100 o00 100 100 Intake 100 100 100 100 100 100 100 IC2 5 Mean Dry Weight (mg)
Test Solutions (Replication) 1 2 3 4 Mean Control 0.525 0.476 0.472 0.433 0.476 10.98% Effluent 0.493 0.520 0.559 0.584 0.539 22% Effluent 0.487 0.455 0.486 0.621 0.512 43.9% Effluent 0.716 0.493 0.510 0.542 0.565 72% Effluent 0.611 0.579 0.465 0.503 0.540 100% Effluent 0.501 0.511 0.516 0.595 0.531 Intake 0.558 0.587 0.541 0.522 0.552 IC2 5 Value: > 100% Calculated TU Estimates: < l.OTUc 95% Confidence Limits: Permit Limit: 1.OTUc Upper Limit = N/A Lower Limit = N/A TUc = 100/IC 25 6
SUMMARY
/CONCLUSIONS Outfall 101 samples collected February 24-March 1, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results indicate that fish pathogen interference is likely the cause of historic problems with WET tests for this effluent.
7
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1) Pimephalespromelas Tests were conducted according to EPA/600/4-91/002 (July 1994) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.
- 2) Ceriodaphniadubia Tests were conducted according to EPA/600/4-91/002 (July 1994) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS/MODIFICATIONS TO TEST PROTOCOL
- 1) Pimephalespromelas None
- 2) Ceriodaphniadubia None DEVIATIONS/MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1) Pimephalespromelas None
- 2) Ceriodaphniadubia None 8
PHYSICAL AND CHEMICAL METHODS
- 1) Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2) Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3) Temperature was measured using EPA Method 170.1.
- 5) The pH was measured EPA Method 150.1.
- 6) Conductance was measured EPA 120.1.
- 7) Alkalinity was measured using EPA 310.1.
- 8) Hardness was measured EPA Method 130.2.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA/600/4-91/002. Any known deviations were noted during the study and are reported herein.
9
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1) Test Type: 7-day chronic tests with results expressed as IC 25 values in g KCI.
- 3) Dilution Water Used: Moderately hard synthetic water.
- 4) Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
REFERENCES
- 1) NPDES Permit No. TN0026450.
- 2) Lewis, P. A., D. J. Klemm, J. M. Lazorchak, T. J. Norberg-King, W. H. Peltier, M. A. Heber.
Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA/600/4-91/002 (July 1994).
- 3) Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
10
Sequoyah Nuclear Plant Biomonitoring February 26- March 5, 2002 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, And Initial and Final Chemistry for Fathead Minnow 7-day and Daphnid 3-brood Chronic Tests
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Used to Control Growth of Microbiologically Concentrations of Chemicals Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-March 1, 2002 Dat;~Sohui<~. ATowerbrom ~PFL-222 PCL-401 Cuprostat- ýý, *0M mg/L TRC ngL CL-363 mg/L PF mg/L Azole rh
________ Ph2s hate 03/12/1998 0.016 Opolymer DMAD-03/13/1998 0,015 -
0ua1 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.0138 03/17/1998 0.020 03/18/1998 0.018 09/08/1998 0,015 - 0,014 0.005 09/09/1998 0.003' - 0.031 0.011 ý-0.021 09/10/1998 0.014 - 0.060 0.021 09/11/1998 0.013 - 0.055 0.019 09/12/1998 < 0.001 - 0.044 0.015 09/13/1998 < 0.001 - 0.044 0.015 09/14/1998 0.008 - 0.044 0.015 02/22/1999 <0.001 02/23/1999 0.005 02/24/1999 0.009 "
02/25/1999 0.012 02/26/1999 0.008 _ H 02/27/1999 < 0.001 i:
02/28/1999 <0.001 08/18/1999 - 0.015 i 0.069 0.024 08/19/1999 - 0.012 0.068 0.024 08/20/1999 08/21/1999 0.023
- 0.022 0.070 0.068 0.024 0.120 08/22/1999 - 0.022 0.068 08/23/1999 0.025 0.068 0.024 0.024 0.006 08/24/1999 0.024 0.016 0.067 0.023 0.020
Table B- I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Chemicals Used to Control Growth of Microbiologically Concentrations of Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-March 1, 2002 Date Towerbrom PýCL;-222 PCL-401 *CL-363 Cuprostat nypqchmonte PF H-I OM m~EC mg/L TRC mg/LgL: i mg/L Azole 01/31/2000 Co..polymrer j3Auivu fQuat~
< 0.002 0.009 02/01/2000 .
0.011 0O.026 0.028 02/02/2000 ,
0.028 0.026 0.009 0.006 02/03/2000 - 0.008 0.027 0.009 02/04/2000 0.006 0.027 0.009 0,005 0.109 02/05/2000 < 0.002 0.027 0.009 - ..
02/06/2000 < 0.002 0.027 0.009 07/26/2000 . <0.0057 0.055 0.019 0.021 07/27/2000 - 0.019 0.055 0.019 -
07/28/2000 0.0088 0.053 0.018 0.004 0.108 07/29/2000 < 0.0088 0.055 0.019 07/30/2000 < 0.0076 0.055 0.019 07/31/2000 < 0.0152 0.055 0.019 0.006 08/01/2000 . < 0.0141 0.055 0.019 0.005 -,
12/11/2000 0.0143 0.025 0.020 o0.005 -
12/12/2000 - 0.0092 0.025 0.020 0.005 -
12/13/2000 < 0.0120 U25 0.020 12/14/2000 <0.0087 0.025 0.020 - - "
12/15/2000 - 0.0120 0.025 0.020 0.005 12/16/2000 . < 0.0036 20.020 12/17/2000 - -
<0.036
<0.0036 0.025:
0.025 0.020 08/26/2001 0.017 0.06 0.021 0.006 _
08/27/2001 <0.0096 0.06 0.021 0.005 0.021 08/28/2001 <0.0085 0.06 08/29/2001 0.021
<0.0094 0.059 0.020 0.005 08/30/2001 0.021
- <0.0123 0.06 0.021 0.005 .
08/3 1/2001 - <0.005 0.059 0.020 ,
11/25/2001 -<0.0044 11/26/2001 - <0.0119 0.024 0.02 005 11/27/2001 . 0.0137 0.023 0.019 0.007 11/28/2001 - <0.0089 0.022 0.019 0.006 11/29/2001 - 0.0132 0.024 0.02 0.007 11/30/2001 < 0.0043 0.024 0.02 12/09/2001[ - T -nnA'n 1 12/10/2001 <0.0042 12/11/2001 <0.0 104 12/12/2001 0.0128 0.024 0.02 0.008 12/13/2001 <0.0088 0.024 0.02 12/14/2001 0.0134 0.024 0.02 0.007
Table B- 1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-March 1, 2002 Date ':"Sodiuiium
- Towerbrom "CL-22> PCL-40.1 'C-,3.63. Guprostat- - .13.ý,
hypochlorte~ mg/ R mg/L-fýL ni,5* C Psa Copolymer PF mg/L m DMAD 1/02/2002 - < 0.0079 > -0.023 0.02 06 .
1/03/2002 <0.0042 0.023 0.014 -,
1/04/2002 0.0124 0.024 0.014 0.009 1/05/2002 - <0.0042 - " .
1/06/2002 " <0.0042 0- .
1/07/2002 - < 0.0089 0.024 0.014 0.006 _
2/24/2002 - < 0.004 2/25/2002 < 0.004 0.023 0.023
- 2/26/2002 - 0.0143 0.023 0.023 0.007.
2/27/2002 : <0.0041 0.023 0.023 2/28/2002 0.0041 0.024. 0.008 3/01/2002 <0.0041 0.024 0.008 - - .
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pinmephalespromelas and Ceriodaphnia dubia Tests, Sequoyah Nuclear Plant Effluent (SQN),
Outfall 101, February 26- March 05, 2002 Temperature Dissolved Oxygen pHu Total Conductance Alkalinity Hardness Residual Test/ Initial Final Initial Final Initial Final Initial Chlorine Sample ID (OC) (OC) (mg/L) (rag/L) S.U. S.U. ([tmhos) mg/L CaCO 3 mg/L CaCO 3 (mg/L)
Fathead/ 24.6 24.4 8.0 7.4 8.10 7.86 315 67 86 Control (24.3-25.0) (24.2-24.7) (7.6-8.2) (7.0-7.8) (8.08-8.11) (7.65-8.02) (309-323) (65-69) (86-86)
Fathead/ 24.7 24.4 8.0 7.4 8.10 7.8.1 308 10.98 % (24.4-25.0) (24.2-24.7) (7.8-8.1) (6.5-7.9) (8.08-8.12) (7.52-8.00) (302-322)
Fathead/ 24.7 24.4 8.0 7.4 8.09 7.80 295 22 % (24.4-24.9) (24.2-24.7) (7.9-8.2) (6.4-7.9) (8.06-8.12) (7.50-8.00) (289-307)
Fathead/ 24.8 24.4 8.1 7.4 8.04 7.78 264 43.9 % (24.5-25.0) (24.2-24.7) (8.0-8.3) (6.5-7.9) (7.98-8.08) (7.49-7.95) (260-272)
Fathead/ 24.8 24.4 8.2 7.4 7.98 7.76 223 - .
72% (24.4-25.1) (24.1-24.7) (8.0-8.5) (6.4-7.9) (7.88-8.03) (7.46-7.95) (220-228)
Fathead/ 24.9 24.4 8.3 7.4 7.90 7.74 180 55 69 <0.10 100% (24.5-25.2) (24.2-24.7) (8.1-8.6) (6.9-7.7) (7.75-7.99) (7.48-7.94) (177-183) (54-56) (66-70) (<0.10-<0.10)
Fathead/ 24.8 24.4 8.3 7.5 7.88 7.77 177 56 70 <0.10 Intake (24.3-25.2) (24.2-24.7) (8.1-8.6) (7.2-7.8) (7.73-7.99) (7.47-8.01) (175-182) (54-59) (66-76) (<0.10-<0.10)
Daphnid/ 24.5 24.4 8.0 8.1 8.10 8.12 315 67 86 Control (24.3-24.7) (24.2-24.6) (7.6-8.2) (8.0-8.2) (8.08-8.1 1) (8.07-8.17) (309-323) (65-69) (86-86)
Daphnid/ 24.6 24.4 8.0 8.1 8.10 8.13 308 10.98 % (24.5-24.9) (24.2-24.6) (7.8-8.1) (8.0-8.2) (8.08-8.12) (8.07-8.18) (302-322)
Daphnid/ 24.6 24.4 8.0 8.1 8.09 8.13 295 22 % (24.4-24.9) (24.2-24.6) (7.9-8.2) (8.0-8.2) (8.06-8.12) (8.07-8.14) (289-307)
Daphnid/ 24.7 24.4 8.1 8.2 8.04 8.13 264 43.9 (24.4-25.0) (24.2-24.6) (8.0-8.3) (8.1-8.2) (7.98-8.08) (8.06-8.20) (260-272)
Daphnid/ 24.8 24.4 8.2 8.2 7.98 8.08 223 72 (24.5-25.0) (24.2-24.6) (8.0-8.5) (8.0-8.3) (7.88-8.03) (7.99-8.14) (220-228)
Daphnid/ 24.8 24.4 8.3 8.2 7.90 8.02 180 55 69 <0.10 100% (24.6-25.0) (24.2-24.6) (8.1-8.6) (8.0-8.4) (7.75-7.99) (7.90-8.10) (177-183) (54-56) (66-70) (<0.10-<0.10 Daphnid/ 24.8 24.4 8.3 8.2 7.88 8.11 177 56 70 <0.10 Intake (24.4-25.3) (24.2-24.6) (8.1-8.6) (8.0-8.3) (7.73-7.99) (7.99-8.22) (175-182) (54-59) (66-76) <0 1.0-<0. 10)
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for PimephalespromelasTest, Sequoyah Nuclear Plant (SQN),
UV Treated Outfall 101 and Intake Samples, February 26- March 5, 2002 Total Temperature Dissolved Oxygen pH Conductance Alkalinity Hardness Residual Test/ Initial Final Initial Final Initial Final Initial Chlorine Sample ID (oC) (OC) (mg/L) (mig/L) S.U. S.U. (jtmhos) mg/L CaCO 3 mg/L CaCO 3 (mg/L)
Fathead/ 24.6 24.4 7.9 7.4 8.01 7.86 319 67 86 Control (24.3-24.9) (24.2-24.7) (7.6-8.1) (6.8-8.0) (7.93-8.11) (7.60-7.99) (308-340) (65-69) (86-86)
Fathead/ 24.8 24.4 7.9 7.4 8.04 7.85 310 10.98% (24.4-25.0) (24.2-24.7) (7.6-8.1) (6.7-7.9) (7.95-8.11) (7.59-7.98) (304-324)
Fathead/ 24.9 24.4 7.9 7.4 8.05 7.82 295 22 % (24.3-25.2) (24.2-24.6) (7.6-8.2) (6.6-7.9) (7.96-8.11) (7.58-7.96) (289-306)
Fathead/ 24.9 24.4 8.0 7.5 8.03 7.81 264 43.9% (24.5-25.2) (24.2-24.6) (7.6-8.2) (6.6-7.9) (7.95-8.08) (7.58-7.94) (260-271)
Fathead/ 25.0 24.6 8.0 7.4 7.99 7.77 222 -
72% (24.4-25.4) (24.2-25.5) (7.6-8.4) (6.7-7.9) (7.91-8.04) (7.55-7.91) (219-225)
Fathead/ 25.1 24.4 8.1 7.5 7.93 7.76 180 55 69 <0.10 100% (24.8-25.4) (24.2-24.6) (7.6-8.4) (7.1-7.8) (7.85-7.99) (7.54-7.88) (177-185) (54-56) (66-70) (<0.10-<0.10)
Fathead/ 25.0 24.4 8.1 7.6 7.92 7.75 177 56 70 <0.10 Intake (24.7-25.3) (24.2-24.6) (7.8-8.5) (7.2-7.9) (7.82-7.98) (7.52-7.90) (174-182) (54-59) (66-76) (<0.1 0-<0. 10)
Sequoyah Nuclear Plant Biomonitoring February 26 - March 05, 2002 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD Page I of 1 Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):
Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN C o nta iner Field Identification o.Grab/ Ship. Collection Date/Time Number & Flow Rain Event?
Sample Description Comp. Temp. (Volume (MGD) (Mark as Appropriate) 0 *~...
C) Collected
.... Si~i End it En ....
~~ ~ ~
y.i Y If Yes, Inch;;.
No TEr ii itT.a rw ~ m ii SQN-101-TOX comp 4
/,p0202 ~ (2.5g)
N."~ .
SQNNT-TOX comp 4 42 / 1 (2.5g) ".. . ...
Sample Custody - Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time t-ed e* oz-z-,o 116s- /,ffi otW Je.-4S Oz,-z, Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 40C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with amnple time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of I Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):
Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN Containr *; * ...
- ..*:*`..*::*.*
Container X Field Identification / Grab/ Ship. ColcinDt/ie Number & Flow Rain Event? -X ...
Sample Description Comp. Temp. ( Volume (MGD) (Mark as Appropriate)
°C_ Collected ... .... . . .
Str - if Yes, No Trace ST.g Av1np(1y ue Apaz
/q ..... .2/27/Qk .......
SQN-101-TOX comp 4 2/26/02 Oft& '!,,(2.5g) ... io'4 i SQN-INT-TOX comp 4 2-/ 1 (2.5g) ~
Sample Custody - Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time
________________ el,2-28 ()O5 _ O29-02 o____________ lO0tS Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 40C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page I of 1 Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):
Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN Container '.
Field Identification / Grab/ Ship. Collection Date/Time Number & Flow Rain Event?
Sample Description Comp. Temp. (Volume (MGD) (Mark as Appropriate) .tt 0_____)_ Collected____
.trtI .. .-.......-....
Y.,es N TramcAwTmp Inch v...-...-......-..-............. ...---.-. ..-..... pe
/5* 3/1/02 ............
SQN-101-TOX comp 4 2m- z5 2 (2.5g) ~Q~ :~
SQN-INT-TOX comp 4 /2970 3/(/02g)
_ __ __ _ _ _ _ _ __ _ O::
- ::::::: ::: :: :: ::: :: :: ::: ::::::: :: :: ::::: :::::::::::::::::D::::
Sample Custody - Fill In -FromTop Down Relinquished By (Signature): Date/Time Received By (Signature): DateTime Saml D C T(Vu)a p t_ 67.... ....
instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 4"C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
.01 Environmental Testing Solutions, LLC 0
p.qpi. 211 Sample Receipt Log Date Time Received Received Sample Project Sample onn Received Received By From Temperature Nme Nubr SplNaendDsitonState Cmet Numbe Number SampleNameanDecito
.o aO'i4, AL*',-, '-.QCAh1Me 2.1*C. Z oZof02 0, 1, E7,AXW EtjV. - IMAIS4AU. LLf lwro C -tic__
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a Page 2-Zfi Environmental Testing Solutions, LLC Sample Receipt Log Sample Date. Received Project Sample SapeNmanDecito State Comments Received Temperature SapeNmanDscito From Number Number Received Received By 0.1Ic "&9 Zi2Z&. ~TVA SekJcjIAW e Febe~x LIV2M -2 ics & Is1.APIT TO~
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L %%o j. v.L u Chronic Whole Effluent Toxicity Test (EPA/600/4-911002 Method 1000.0)
Species: Pimephalespromelas Client: -WT , AA AXW ?*AM-.
Facility: No/e 'RiaATeb NPDES #: -M 002OW4SQ, Mh batch: O T-I-oY* -
U2J-.,-I_7-,
IDilutionpccp(%)
eI.q*I Dilution preparationinformation:
EfenvW(mL) IS1.1 2Z
-36 17_
145z.2___________
t Comments:
Diluet Volume (mIQ ,4 q2E 1I.
Test organism information: Cv 64t Test information:
Organism age: 2321f4piS Ot Randomizing template: G Date and times organisms 25Z- 1-Ot 1530 7;6 4¢* " Incubator number: z were born between: I oO r-Sr
" oo "S3 Organism source: ASS L*.tC *?-is:6Z. Artemia lot number: iP du-??
Transfer bowl information: pH = Temperature = Oven temperature: 60.
7M21.V C- Dryig time:
Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time i GZ-Z."-0o. 0*i1qO 1" 1 I 2 a cjaq-t-29Q W41 q5D /4 I, 3 Oaz" e -~o) IWO I* A AvrA r.0O 0w 6' 654;c. PA 1(
%_Morality ito o <1~c _0__7day_ C50____'
IControl infoirmation:
5>0.25 Acetac criteria Summar of test endpoints NOEC 0*f Average weight pr larvae: 0. moarvae LOEr -7/00 -,Z, ChV > /0o IC25 > /60%
Environmental Testing Solutions. LILC I S........
Envronentl S s , -rage tng 2 of 8 Species: Pimephalespromelas Client: "T-VA- Seja-ANtA Date: OZ-zg0.7 Survival and Growth Data Day CONTROL __.4o % 2z A B C D E F G *H J K L 0 tO *l0 0 10 /0(010 /Q)" /0 I0 'ow/
1 /0 o -0/0 0 to 10 to /0 10/0 0 /0 2 0!
0o /0/0 / 00 /0 16 10
/0 /0 /0 10 /0/0 1
/6 o ,0 /0 /0 10 10 10 to qI0 IL)10 (5i IN 10 I1 10 !0 10 lo Io 10 10 10 "14 IQ !0 /0 6
7b 10 1i 1 10t 10 lb 1010 10 tO/
B = Pan + Larvae weight (mg) 6 A(
Larvaeweigjhit(mg)=A-B .E&L j06 20 . 7.'4 - *. , * .
Calculations and data reviewed:
Comments:
- Vi.
Environmental Testing Solutions. LLC rage 3 Of 8 Species: Pimephalespromelas Client: "r"VA- _nUa 4AA Date: 0?-- ZIP-Oz Survival and Growth Data Day ,77- 76 106 M N 0 P Q* R S T U V rW eX 1 // A) IJ 1 0/ 01 0 /0 /1 I0 / 0 1c/
/0 /0 /0 10 I0' /0 10 10 I0D /0 /0
_0 /I0 /o (0 10 0 10 /O /0 (01o'0
/_o 10 0
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/010 O 1O0 to/0 /0 7Calcula0 /0 10 tin /0da/t r/v"e" Baclain =n +e Lavewigt(g dPta wd Comments: -)t da.
- 0 -- -
,,nvhvnmeIILaU bnvtronmemm eSLIUr.. Q 10 IUL . U113, I I I
- 1, Ims Species: Pimephalespromelas Client: -TVP S_*nuiNAA Date: OZZ,*,O*
Calculations and data reviewed: 4 Comments:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pinephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, Nontreated Test dates: Febr uary 26- March 5, 2002 Project number: 269 Revelwed br. /' I Concentratton (%) Replicale tttialtnumberof Flaal nwberoftarvae A - Pau ightt B - Pan Larae Larvae wesht n() Weight /liiala number Mea urvtval Mas weltkl (n) CndmtO Percet rndedain A*=
larvae (ag) .eighl (.&) -A-B oftava¢(nmg) (%) nrall (%) mI (%)
A 10 10 15.038 22.060 7.022 0.7022 Control B 10 10 14.940 21.400 6.460 0.6460 100.0 0.6151 14.3 Not applale C 10 10 14.970 21.150 6.180 0.6180 D 10 10 15.070 20.010 4.940 0.4940 E 10 9 14.708 20.260 5.552 0.5552 10.98% F 10 10 14.848 20.820 5.972 0.5972 86.4 0.5232 25.0 14.9 G 10 10 14.715 20.820 6.105 0.6105 H 9 5 14.681 17.650 2.969 0.3299 1 10 10 15.085 20.880 5.795 0.5795 22% J 10 10 14.646 20.160 5.514 0.5514 100.0 0.5471 4.5 11.0 K 10 10 14.657 19.880 5.223 0.5223 L 10 10 14.627 19.980 5.353 0.5353 M 10 10 14.727 20.440 5.713 0.5713 43.9% N 10 10 14.621 19.920 5.299 0.5299 100.0 0.5675 7.5 7.7 0 10 10 14.847 21.110 6.263 0.6263 P 10 10 14.834 20.260 5.426 0.5426 Q 10 9 14.936 20.730 5.794 0.5794 72% R 10 10 14.814 21.000 6.186 0.6186 97.5 0.6128 9.6 OA S 10 10 14.800 21.730 6.930 0.6930 T 10 10 15.039 20.640 5.601 0.5601 1 U 10 10 14.851 21.700 6.849 0.6849 100% V 10 10 14.981 21.240 6.259 0.6259 92.5 0.5874 23.1 4.5 W 10 10 15.051 21.570 6.519 0.6519 X 10 7 14.642 18.510 3.868 0.3868 Y 10 10 15.095 20.460 5.365 0.5365 100% Intake Z 10 10 14.983 21.800 6.817 0.6817 100.0 0.5834 12.3 5.1 AA 10 10 14.945 20.190 5.245 0.5245 BB 10 10 14.910 20.820 5.910 0.5910 1 1 Outfall loll MSD = Minimum Significant Difference Dunnett's MSD value: 0.1544 PMSD: Percent Minimum Significant Difference PMSD: 25.1 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and beatracnt that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occus for Environmeaal Jntake. Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.5% from the control (determined Dunnctl MSD value: 0.1102 Lower PMSD bound determined by USEPA (10th percentile) = 9.4%
PMSD: 17.9 Upper PMSD bound determined by USEPA (90th percentile) = 35%
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laborato:ies for'Pimqhalea growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Proteclion Agency, Cincinnati, OIf.
Environmental Testing Solutions, LLC Statistical Analyses Str Dar. 2/2a02 lanrva F=J Growth ad Sa-wvval Ta-7 Day Surviva Teat M: PpFRCR End Datt Samn* 1D" IVA SQN, Oufal 101 - NouIa-emd 3/St02 Lab Mt MTS-Env. Testing Solutions Sample TyDpe:M,-Discharge Monitoring Report Sample DaW. Protoco: EPAP 9 -EPA Freahwatm Tea Specim: PP-Pimephales premects Commenm:
Come-% 1 2 3 4 D.Conutol 1.0000 1.0000 3.00)0 1.0000 10.98 0.9000 1.0000 1.0000 0.5556 22 1.0000 1.0000 1.0000 1.0000 43.9 1.0000 1.0000 1.0000 1.0000 72 0.9000 1.0000 1.0000 1.0000 100 1.0000 1.0000 1.0000 0.7000 Transform: Arculn Square Root Conc-% Mean N-Mean Mean Rank 1-Talled Mi Max CV% N Sam Critical D-Control .0000 1.0000 1.4120 1.4120 1.4120 0.000 4 10.98 0.8639 0.8639 1.2285 0.8411 1.4120 21.936 4 22 1.0000 1.0000 14.00 10.00 1.4120 1.4120 1.4120 0.000 4 18.00 30.00 43.9 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 72 0.9750 18.00 10.00 0.9750 1.3713 1.2490 1.4120 5.942 4 16.00 10.00 100 0.9250 0.9250 1.3068 0.9912 1.4120 16.103 4 16.00 10.00 Auxiliary Tests Shapiro-Wilk's Test indicates non-normal distribution (p statistic Critical
< 0.01) Skew Kurt 0.75127089 0.884 -1.747871287 4.247234099 Equality of variance cannot be confurmed Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test to0 >100 I Dose-Response Plot 0.9 0.8 0.7
>0.6 S0.5 I*0.4.
0.3 0.2 0.1 0
r*N sqnO2-26-02.xls
Environmental Testing Solutions, LLC Statistical Analyses SWDm 22&W2 LSaVYlFh Grnwlhlad Sunfrui Tes8-7Day Grmh TomID.
8mh 1D TVA SW. Ow 101. N- atod EWdDUE 3/=~0 LabIDk ITS-W. Tuft So1WIOII SAM*piTwo S=Wk Dow PtImOOL. DMR.Dkdmar M-WzingoR" EPAT91-EPAPRubhw Ted Specdt: PP-Ptdwm h prowd" Cmo.% 1 2 4 13CO1 0.7022 0.6460 0.6180 0.4940 10.9 0.s52 0.5972 0.6105 03299 22 0L75 0.5514 O023 0.5"353 43.9 O.513 03.5299 O.6263 05426 72 0.79 0.6186 0.6930 0,5601 100 0.6849 0.6259 0.519 03868 TranfoM UnraISMlmed Cono.% Me* NMM Mean Rank 1-Talled 11o0o41C 4 Max CV% N D..Czarol 0.6151 Sum Critkcl Man 1.0000 0.6151 0.4940 0.7022 N-M..
14.302 4 10.98 0.5232 0.8507 0.5232 0.6151 1.0000 03299 0.6105 25.040 4 22 0.5471 0.8896 13.00 10.00 0.5676 0.5471 0.5223 0.57O5 0.9229 4.505 4 14.00 10.00 43.9 0.5675 0.9227 0.675 0.5676 0.9229 0.5299 0.6263 7.548 4 72 0.6128 15.00 10.00 0.5676 0.9963 0.6128 0..601 0.6930 0.9229 9.589 4 17.00 10.00 1OD 0.5874 0.9550 0.5874 03868 0.5676 0.9229 0.6849 23.133 4 10.00 10.0) 0.5676 0.9229 Auxiliary Tet Shapi,-Wdks Tet imdicaa nonnal di'nibo' Statiutic Critical (p ' -0,01) Skew Kur 0.87742573 0.384 246332l7 BauldensToa u equal vjnraow (p - 0.10) 1.425134333 9.240524292 . 15.08631706 Hypodwis Tat (1-44 0.09) NOEC LOEC ChV TU Stad's Mwy-Oo Rank Test 100 '100 1 Point im-a Instpolaon (9) RIsnpift)
% SD 9"% C*(Exp) Skew 1C05" 7.1161 ICIO >100
[Cis '100 1.0 1C20 >t00 1C25 >100 0.9 IC40 >100 IC50 0.8
>100 0.7 ndCates IC csbtazc l=ss han otelowenconom~on0.
a0.6.
0.5 0.3 0.2 0.1 .
0.0 . .
0 20 40 60 80 100 120 Dose %
Dow-Rlsponoo Plot 0.8 0.7 0.6 S0.5 0 0.4 o 0.3 0.2 0.1 0
sqnO2-26-02.xis
Environmental Testing Solutions, LLC Statistical Analyses Used fhr PMSD calktisn oaly.
Larval Fbh Growth and Survival Ted-7 Day Growth Start Da* 2=2&02 Tet ID, PpFRCR Sanple aD TVA SQN, Outfall 101 - Nontreated End Doc 3/%i02 Lab D: ETS-Env. Tesing Solutions SapT Tpe: DMP-Disch-ge Monitoring Report Sample Date Protocol: EPAF 91-EPA Fr*dbwur Test Speciea: PP-Pimephaltz 1110e11 Comments:
Cone-% 1 2 3 4 D-Cotrol 0.7022 0.6460 0.6180 0.4940 10.98 0.5552 0.5972 0.6105 0.3299 22 05795 05514 0.5223 0.5353 43.9 05713 0.5299 0.6263 0.5426 72 05794 0.6186 0.6930 0.5601 100 0.6849 0.6259 0.6519 0.3868 Transform: Unbansformed Conc-% Mean N-Mean Mean 1-Tailed MiR Max CV% N t-Sat Critical MSD D-Conttol 0.6151 1.0000 0.6151 0.4940 0.7022 14.302 4 10.98 0.5232 0.8507 0a5232 0.3299 0.6105 25.040 4 [.434 22 0-5471 2.410 0.1544 0.8896 0-5471 0.5223 0.5795 4505 4 1.060 2.410 0.1544 43.9 0.5675 0.9227 0.5675 0-5299 0.6263 7.548 4 0.742 72 0.6128 2.410 0.1544 0.9963 0.6128 0.5601 0.6930 9.589 4 0.036 2.410 0.1544 o00 05874 0.9550 0.5874 0.3868 0.6849 23.133 4 0.432 2.410 0. [544 Auxiliary Tests Statistic Critical Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) Skew Kurt 0.87742573 0.884 Bartletfs Test indicates equal variances (p = 0. 10) -1.24633217 1.425134333 Hypothesis Test (1-tail. 0.05) 9.240524292 15.08631706 NOEC LOEC ChV TU MSDu Dunnetfts Test MSDp MSB MSE F-Prob 10t >100 df I 0.154409948 0.251052676 0.005359172 0.008210062 0.663285613 5, 18 Dose-Response Plot 0.8 0.7 0.6 0.5 1-tail, 0.05 level
- 0. 0.4 of significance o 0.3 0.2 0.1 0
sqnO2-26-02.xls
Environmental Testing Solutions, LLC Statistical Analyses Larval Mld GrowtM and Swvtval Tat-7 Day Growth St= DaW 2/26&02 Ted ID PpFPRCR Sample ID: TVA SQN. Inake -Nonneated nd Dar 3/5o Lab ID. ES-Emv. Teting Soludong SaMp Tý:p DR.-Diachage Monhtoing Repo Sample Dat Pwtocol: IAF 91-EPA Freshwa Coinn mi- : Test Species: PP-l'mePlas P-3-dM p ed Cow-% ! 2 3 4 40.4940 jCOMa1 0.7022 0.6460 0.6180 0.4940 0.5910 100 0.5365 0.6817 0.5245 0.5910 Transform: Untranwformed Conc-% Mean N-Mean Mean 1-Tailed Mi Max CV% N t-aSlt Critical MSD D-Contol 0.6151 1.0000 0.6151 0.4940 0.7022 14.302 4 100 0.5834 0.9486 0.5834 0.5245 0.6817 12.276 4 0.558 1.943 0.1102 Auxiliary Tests Statistic Critical Skew Shapiro-Wiks Test indicates normal dist.ibution (p> 0.01) Xurt 0.962760925 0.749 -204880953 F-Test indicates equal variances (p = 0.74) -0.576806113 1.508488178 47.46834564 Hypothesis Test (I-tail 0.05)
Homoscedastic t Test indicates so sigoifcan differesces Dose-Response Plot 0.8, 0.7, 0.6.
Oz, 1-tail, 0.05 level 0 0.4. of significance a 0.3 0.2.
0.1 01 S
9 0
sqnO2-26-02.xls
Environmental Testing Solutions. LLC Pso* I rW*
Speai CI o h V Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1002.0)
Species: Ceriodaphniadubia T a Date Time Analyst Client:rVW\ 5eja%)0,Mk &r""je~ P~r Test start tet end C'k2--26.0.
0 O- -oz0t02 IS Is .. I 1Z Facility:
NPDE S #: "'
AMdAJx-eA-et
- o _~
- Dilution prpartioninformation: Comments:
MHS batch: /-a.OZ -zj.._
!o i.-. prep Ib~ 2t q.,_ 17. Io _
Ef,,
uvo*hme (mL)
Diluast vohmme~L 1L.71S c'l*46 2 3~ 0 ________
Test organism information: Test informatown:
Organism age: '-7(A.- AooUr aRandomizing template: &Ue Date and times organisms C .2-z-O Oh1o 1 `o 1Z3G Incubator number:
were born between: 2 Organism source: Oz- "q-zA**6 YCT batch: A19" tIQ-2-Z Transfer bowl information: pH = *i Temperature = ?sq.q Selenastrum batch: AS6 zZ-7-"
CONTROL Survival andReproduction Data Replicate number Day 11 2 3 4 5 6 7 8 9 10 1 Young produced 10 Q 0
___ 0 G (D_.
Adult mortality L_. L_ L. _
2 Young produced p n G 3 Young P ouced- 0.
C) 1&jj A/ 1z4 Adult mortality L ILL L._ L L 4 Young produced -7
[ [1 J2L- _L. I_
6 Young produced Adult mortality Adult mortality I
~
L -
~ u_ _ _ U _ -- L'- I L L - K
____ LLU 6 Young Produced 1211 4 2- iIL Fid Adult Mortality -_ =. __ _ __
X for Broods Calculations and data reviewed:
Test Renewal, Feeding, and Incubator Location Infornation Mon Tue Wed Thu Fri Sat Sun Time .* szz '-A Ism Shelf ILocation L b A-I Control information: Acpe criteria Summary of test endpoints:
%of Male Adults. 20% 7-day LC50 "It Oo0 7
%Adults having 3" Broods: / ' .80% NOEC 1OK
%Mortality: *520% LOEC > 100 Mean Offspring/Female: 5.
5.' Ž15.0 offspring/fetmae ChV > 1_007.
% CV: 91% < 4o.0 % IC25 > t_____
Environmental Testing Solutions. LLC Page 2 of 8 Species: Ceriodaphniadubia Client: -&SA S_& tee$ Date: 0Z-2(o-oZ.
CONCENTRATION: WAS% Survival andReproduction Data
__ ~ number_
Day 1 2 3 4 5 6 7 3 9 10 Alartak .- .- - -L- L- L- I-- L 1 Young produced n C C) n, p Adult mortality __ . _
2 - Young produced - - X-- - -
o tr0ti - -
AdulMmortaty Iiy L 3 oudy Aduhmrbtat gproduced n 20 L 737 L- z0 C>U U UZ25 U 0*Z 2 3 Young produced /0 1 0 1 0 n 0 4 You*ogproduced O 0 2) 1 I Q 1___ _
Total young produced 21 Youngrproducedl":
22 23 G =.'9 2z3 "2-Mea V- 2Z2 a 2S, zq FinalAdult Mo ty =
Concentration:
35 Adlult mortality Youg produced C TJ U' U U/*-
I% U Mortality:
% Reduction from Control:
J L!,-- U
- 1--
oT.4/
I 1
Calculations and data reviewed:
CONCENTRATION: 27?_
iz* 0 (
Survival 0and 0 Reproduction I3 DataIZt a 6 Young produced /0
__________Replicate number Day___ 1 2 13 4 15 16 17 18 19 1 10 I Young produced 61 __ __0C fl Adult mortality YoungproJduced Adultmortalty L4 L.. _
o I 4U-(
2n 1U I'
_ 14.1...1
_- L 3 Young produced iLL 11.3 Adl otaiy I L- I - '- I =-L o1014 1 ,-3 1 A IX1 4
1 Young produced Adult mortality I U L 6
~mortaity Yugpoue13 L BI~ L 12- 1 1111-IKI~ 1 IZ_____ 6_____
H~
0_____ 1-5____
Adult mortality U _ LLL 67 Young produced1_1kIi dlmraiy~ V 4 1~I~I~I~ TF I Z1 1_ 0
- Total young produced I ý 24 -..- 2 - -2 Zt 2 5 S i l FbWa Adult Mortality _____t -__ I I C*
Concentration:
%Mortality: -0CA Mean OffspringFemale: zq.z
%Reduction from Control:-3.q '
Calculations and data reviewed:
Environmental Testing Solutions. LLC F.aze 3 of 8 Species: Ceriodaphniadubia Client: -t-r(A Pe-CUoAi go Date: 02,. z,-o-..
CONCENTRATION: Survival andReproducin Data Day !1 , 3 4 5 6 7 8 9 10 1 Young produced 0 0 Adult mortal.ty 2 Yo~ungproduced 0C)~6 r~ c .
Aduhtmortality L -L C W T L L 3 Young produced 0 0 0 0 1 '10A. 0 M' m -L --.-- L
- _ - ..
4 Young produced .QdL.
Adlmortadly C
-5
. 0 02 5 Young produced 2. ,, " _ 2 _
Adult mortality L .. _ .. U
- L _. L 6 Young produced L5 J2- 1j 0 A, o 17 1,3 __
Autmortality L. L- L L \.- %--_ L- t.
7 Young produced 014 e) - O Total young produced Z 8' 3 2L zc. -z' 27 Z 24 22 Z& 21 Final Adult Mortality L- C? t U7 ~ W-? C Concentration:
% Mortality: I Mean Offspring/Female: 2S;.5
% Reduction from Control: - '6.
Calculations and data reviewed:
CONCENTRATION: and Reproducon Data Replicate number Day 21, 3 4 6 77'Q 88 4 i 5 6 990 10
()10.
I Young produced 3 2 '
Adult mortality Young produced II ~t 5e-t~l (_ U . T t.
3 Young produced __AlI5 =j-T3_
= Adultmortality L- L L Adult mortality t.. .
Adult mortality LI I L- _
45 Young prouce /0 'i//' 0 16 ? I)1_/0 i6 To4a Young producd tITA1JZ T '11II-Young produced- 1AdultMortalt c L.iL 2 I0 3'(, L (p L I A4 0 31 45 e-)
Adult mortality TZ
=7 Young produced C.10 .- O 4 J7I ~
Total young produced j So 26 Jo 26 ' 260Z7 -3) ~31 2-P Fina Adult Mortality TC C ~~ t Concentration:
% MortalJtT': 0%
Mean Offspring/Female: 2f. 2
%Reduction from Control: - 20.V, Calculations and data reviewed: 14ý
SnIntinnL LLC Page 4 of 8 E'rnlw.nmontul T'qting Species: Ceiodaphniadubia Client: -A P A- h Date: )2- i,-O 2 CON CN ON: 4off Survival and Reproduction Data
_______number Da 1 2 3 4 5 6 7 8 9 110
.____ ,,,,,_____. t .-
,LC_...
.. 5 0.. .
._L ..
C.-._-. .. -.,
1 You*gaproduced _
Ad.ft morta,,ty I_ _ %-- L. U. L L. .. U
- u mom. -.- L- _- - -L-3 Youproduced 0 4 00 0 2 Yo, producd tO C) d " 1 1-I 0 '0 L L- L. L-Mortalty Adult 4
Adltrb-tYoung produced - I - - 5 L- - t 4 Young produced 7T-0 /0. /-Ia /C 0 /0f 1(.
7 Young produced C) IC) ~ Q..L./~.L12.
Adult mortality
-% - -utonfo Cnrl - q.t*
Totalyoungproduced -z 2-1 Z1 z9 31 30 29 21 7-P TlationsL Adulo and eewd Uaa Final Adult Mortality ...
Concentration:
%Mortality: z')I
%Reduction from Control: - -24.717 Calculations and data reviewed:
CONCENTRATION: -=*7%jAJT7I ke- Survival and Reproduction Data I Young produced () 1 C.)10 1nn 1 2 Young produced 01 '0 n 3 Young produced 4 51910113 Adult mortlt Mean Offspring/Female: .21 4 Young produced 0D 0 D 0 4 5 Young produced q /(1 12-. 12z A- /0 1 6 ~Young produced /./ / I i~/1 1 1 Yugpouced fil0 0 0 0 Total young produced 2(1 2-7 2, 2-1 3o 2 31 1231 31 Flinal Adult Mortlt L- L L c Concentration:
I% Mortality:
%Reduction from Control: - XS7.
Calculations and data reviewed:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses lient: TVA Sequoyah Nuclear Plant, Nontreated est dates: February 26 - March 5, 2002
- oject number: 269 Reveiwed by.
concentration Replicate number Survival Average reproduction Coefficd of Peremt recduIon from
(%) 1 2 3 4 5 6 7 8 9 10 (%) (offsprIngfemale) ()
Control 22 25 26 23 24 22 26 21 20 25 100 23.4 9.1 Not applicable 10.98% 21 22 23 23 23 24 22 23 28 24 100 23.3 &1 0.4 22% 26 24 24 23 24 21 25 25 23 27 100 24.2 7.0 -3.4 43.9% 28 23 26 26 29 22 24 22 28 27 100 25.5 10.2 -9.0 72% 26 30 26 30 26 28 27 30 31 28 100 2&2 6.9 -20.5 100% 31 32 27 29 28 31 30 29 27 28 100 29.2 6.0 -24.8 100% Intake 26 27 28 27 30 29 31 23 31 30 100 28.2 9.0 -20.5 ItfalL 101: MSD = Minimum Significant Difference innett's MSD value: 2.063 PMSD = Percent Minimum Significant Difference ISD: 8.8 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treaftent that can bedeclared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests nAct when a toxicant reduces Ceriodaphnia reproduction by 13.0% ftom the control.
nnett's MSD value: 1.809 Lower PMSD bound deternined by USEPA ( 10 thpercentile) = 1I%.
7.7 Upper PMSD bound determined by USEPA (90d percentile) = 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted fiom 33 laboratories for Cerlodaphnia reproduction in chronic reference toxicant tests.
EPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental
,tection Agency, Cincinnati, OH.
Environmental Testing Solutions, LLC Statistical Analyses SestD 22 TauID:
1 CdPqo Sasg4.D TVASQKOOfail 101- Noataed
&A DMa 3/=2 La M. Es-Ev. TMtiM SOhitla Saqe0t0ps Ma-Di ge Matzitm-i Rq Smuh d PDOa*: EPAP 9l-OlA P mdwa. Tat Spede: OCeD q da& a Cme-% 1 2 3 4 S 6 7 8 9 D.CO~l 22.000 25.0D0 10 26.000 23.000 24.W0 22.000 26.000 21.000 10.98 21.000 20.000 25.000 220 23.000 23.O00 23.000 24.000 22 26.000 22.000 23.000 281.000 24.".0 24.000 24.000 23.000 24.000 21.000 2.000 43.9 2&000 25.000 23.000 27.000 23.000 26.000 26.000 29.000 22.000 24.0W 72 22.0 21.000 27.000 26.000 30.00 26.000 30.000 26.000 20.000 27.A 30.000 31.000 2&000 t00 31.000 32.000 27.000 29.000 21.000 31.000 30.000 29.000 27.000 28.000 Transfrorm UnOUV-fOrmed--
Come-% Mme N-Mm Meam Mu Tailed 150te Max CV/. N t-Slut Czil, D-Conn 23.400 MSD Mmw N-Mm 1.0000 23.400 20.000 26.(00 9.054 10 25.633 10.98 23.300 0.9957 23.300 21.000 1.0000 20.03) 8.105 10 0.111 2.287 22 24200 1.0342 2.063 25.633 1.0000 24.20 21.000 27.000 6.969 10 43.9 -0.877 2 2.063 25.633 1.0000 25.500 1.0897 25.500 22.000 29.000 10.168 10 -2.327 2.28M 2.063 72 28.200 1.2051 25.633 1.0000 28.20 26.000 31.000 6.852 10 -5320 2287 2.063 25.633 1.0000 100 29200 1.2479 29200 27.000 32.000 5.997 10 -6.428 2.287 2.063 25.633 1.0000 Auxilzary Tests Kolhogomv D Tea indicatm noma disnmbion (p >0.01) Sutatit Crtieaw Skew Kurt 0.84679538 1.035 0.130211394 Bar-dedtsTet indicats equal vm*-wem(p - 0.801) -0.644187356 Hypotihesh Test (1-tb"l.0.05) 2.247287512 15.08631706 NOEC LOEC ChV TU MSIU Dulnezs Teat MSDp MSB MSE F-Prob df 100 >100 1 2.06316906 0.088169618 63.62666667 4.07037037 1.7E-09 5,54 Unear
-- tepsatlido(00 Resmnsps)
Point % S 95% CL Skew IC05 >100 ICIO >100 IC15 >100 IC20 1.0
>100 0.0 IC25 >100 0.8 IC40 >100 0.7 IC50 >100 0.6 0.5.
w 0.4 a-0.3 0.2 e 0.1 0.0
-0.1
-0.2
-0.3
-0.4 0 20 40 60 80 100 120 Dose %
Dose-Respose Plot 35 30 25 C
1-tail, 0.05 level 20 of significance 10 10 5
sqnO2-26-02.xfs
Environmental Testing Solutions, LLC Statistical Analyses Sat Daer 2(2 ce ilodagiaiSun'hviluad Rirodwedgo Te ft-Rq-od Z s Teat 1D CdFRCR End Dauz SipM IVl WA SQN, hnaka - Nonreueed 3/5102 Lab ID: MawS-Fa.Testing Solutions Sample Type SAp Dam DMR-Dischwge M*ntoring Rcp Protocol EPAF 9 I-EPA Freshwatw Test Species: CD-Ci dubia Comments:
Come-% 1 2 3 4 5 6 7 0-Comnn 22.000 $ 9 10 25.000 26.000 23.000 24.000 22.000 26.000 21.000 20.000 100 26.000 27.000 28.000 2.00 27.000 30.000 29.000 31.000 23.000 31.000 30.000 Cone-% Transform: Untramernned Mean N-Mean Mesa MIM 1-Taied Max CV% N t-stat D-Control Critical MSD 23.400 1.0000 23.400 20.000 26.000 9.054 10 I00 28.200 1.2051 28.200 23.000 31.000 8.971 10 -4.600 1.734 1.809 Auxitiary Tests Shapiro-Wilk's Test indicates notmaj dest'iburion (p > 0.01) Statistic Crtical Skew Kurt 0.934985161 0168 F-Test indicates equal variances (p = 0.61) -0.567916859 -0.329037901 1.425742626 6.54108572 Hypothesis Test (t-Utl, 0.05)
Homoscedastc t Test indicates no signifcant differences Dose-Response Plot 35 302 25 0
20 1-tail, 0.05 level of significance 15.
10 5-01 sqnO2-26-02.xls
Environmental Testinit Solutions. LLC Page 1 or"8 Chronic Whole Effluent Toxicity Test (EPAI600/4-91/002 Method 1000.0)
Species: Pimephalespromelas Client: 7rA Sao AA e (?A,%rr Facility: V-- ri -eh -...
NPDES #: .I*4- O(2 - - :"
Dilution preparationin ornation: Comments:
M batch: 0-2-t.1OZ 102-2-'LZ. Ep4L ,d, .
JQka, V VIuuVLvuwzI- L ¶tJ-I A23.6If MAAA. Zt--lj.. tm 0iJ w - m k-)
"' 135,"I. "*L K.*-*" Z pf-d #'*tv,, *3,._ ,,,.,,-
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Test organism information: C,,- 6U-o3-6-_ Test information:
Organism age: , "ZU OLYO Randomizing template: GLUe Date and times organisms Gz.ZS'OZ i1O TO tSco t(£* Incubator number:
were born between: 153o - 1-7 G:Sr _
Organism source: A6S 8ATCM 02- zi-O2. Artemia lot number: 6&tz Transfer bowl information: pH = Temperature = Oven temperature: t.o'0
.q 7-3 4t 2 Drying time: - 5 Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 0~a IZ2~O I'7 ASO 2 02-zf-o2. 1q56
________I IO66 3 ___-_1_ Otso 10o0 ,0__
4 5-0 -07* 10 oo100 5 *.O-.o-2. 0 t;0 1514 IqII*. K400 7 S.N.O.?-O 0,, 415) I "0h Controlinformation: Aceptance criteria Summary of test endpoints:
%Mortality: I o *20/% j7-day LC5O > 100 Average weight per larvae: - q Ž0 0.25 miarvae NOEC /O0%'.
LOEC ChV j>
IC25 I> I (&I.*,
Environmental Testing Solntinnt I .Tr
.. . ....- 1 Page 2 ots Species: Pimephalesproma/as Client: TVA-*p(Qut.AH Date: o 2- -..
t.JUV-roePqTer*
Surtival and Growth Data Day CONTROL - O,_ 07 A B C D E F G H I J K L
/0 /o /0 /0 /0 /0 /0 /0 /0 /0 /0 t0 1 /0 /2 /0 /0 /0 /n /0 /0 /0 /0 /0 /0
/0 /0 /0
/I /0 /0 /0 /0 /0 /0 /0 /0 3I/O 6 o0 I0 t0 /0 /0 I0 /0 /0 /C) to to to I0 D i0 to c) IU 10 1O10 1O 1C 5 0 1o to 0 i 0 I /0 /0 q 14 6 lo o0 /0 /0 /0 16 '0 tC/6 /
IL0 /0 0
7 /0 JO /0 / 1/0 /0 /0 /0 /0 /0 /0 A -tPan weight (mg)
-wed: r4'-
B- Pan+ Larv~ae weight (mg) CA 11\ "I? ___j, Larvae weight (mg) -A -.B jiic - -__
Calculations and data reviewed:
Comments:
Environmental Testing Solutions, LLC Page 3 of 8 Species: Pimephalespromeias Client: -WA --Se DuoVa4 Date: 0)22-.*p-oi --rleATeb Survival and Growth Data Day aq 727.
M N 0 P Q R S 0
T U V W X
/6 /0 /0 /0 /0 /0 /0 /0 A0/0 /0/0 1
/0 /0 0 /0 /0 /0 /0 /0 2 /0 /) /0 /1 0
/0 /0 16 /0 3 /0 /0 /0 /0 /6 /0 /0 /0
/0 /0 IC C 4 '0 '0 I0 ,o )
1(0 0O It 5 I0 l"o 10 10 /0 ito I 6 t. 65 to 10 '0 '0 /0 t0
/0 /0 I0 /0 7 IA 0 IC) /0 /0 1 01 0 10 /0 10 /O.
A - Pan weight (mng)
A Or
/0 6 /6 16 B = Pan + Larvae weight (.ag)
Larvae weight (ag) A-A B alculations and data reviewed: 6k Comments:
Environmental Testin2 Solutions, LLC 5
skUi -t VL 0.
Species: Pimephalespromels Client: -frVA - S*un.wq,4 Date: O2-goz.
VV- rr'e*ere-b Surival and Growth Data Day 1o06% mrA Ke.-
Y Z AA BB C DD EE FF GG 11 II JJ 0 A) 1/0 /I0 1 ,0 z 10 /0 /0 /0 2
/
3
/0 /6 /0 /0 4 /0 lo1oJo I 5
10 10 o 6
7 A -Pan weight (mg) -
B - Pan +Larvae weight (mg 4,~
Larvaeweight(mg)-A-BB . .0 I[o Calculations and data review Comments:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pinmephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, UV Treated Teat dates: February 26 - March 5, 2002 Project number: 269 Rcvdweod by:__________
Coe.t,-raton (%) lumberof dat FInalnumbroflavae A - Pan weight B = Pan + Larvae Laae wlht (rag) Weight/ IIital aumb- Mean sarival (m& Ce I larvae (nwj weight (nag) A -B of Iarva (Mg) (%) () e"" (%)
A 10 10 14.787 20.040 5.253 0.5253 Control B 10 10 14.820 19.580 4.760 0.4760 C 10 10 14.645 19.360 4.715 0.4715 D 10 10 14.993 19.320 4.327 0.4327 E I0 10 14.696 19.630 4.934 0.4934 10.98% F 10 10 15.007 20.210 5.203 0.5203 G 10 10 15.048 20.640 5.592 0.5592 II 10 10 14.841 20.680 5.839 0.5839 1 10 10 14.657 19.530 4.873 0.4873 22% J 10 10 14.687 19.240 4.553 0.4553 K 10 10 14.682 19.540 4.858 0.4858 L 10 9 14.917 21.130 6.213 0.6213 M 10 10 15.057 22.220 7.163 0.7163 43.9% N 10 10 14.754 19.680 4.926 0.4926 100.0 0.5654 1812 -187 O 10 10 14.886 19.990 5.104 0.5104 P 10 10 14.977 20.400 5.423 0.5423 O 10 10 14.714 20.820 6.106 0.6106 72% R 10 10 14.662 20,450 5.788 0.5788 100.0 0.5395 12.4 -13.3 S 10 10 14.866 19.520 4.654 0.4654 T 10 10 14.808 19.840 5.032 0.5032 U 10 10 15.016 20.030 5.014 0.5014 100% V 10 10 15.031 20.140 5.109 0.5109 W 10 10 15.010 20.170 5.160 0.5160 X 10 10 14.949 20.900 5.951 0.5951 Y 10 10 15.271 20.850 5.579 0.5579 100% Intake Z 10 10 14.895 20.760 5.865 0.5865 100.0 0.5516 5.0 AA 10 10 15.025 20.430 5.405 0.5405 BB 10 10 14.654 19.870 5.216 0.5216 MSD Minimum Significant Difference Dunnett's MSD value: 0.1109 PMSD - Percent Minimum Significant Difference PMSD: 23.3 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and traetmnt that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for EFnionmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.5% from the control (detennined through Dunnett's MSD value: 0.0456 Lower PMSD bound determined by USEPA (10th percentile) = 9.40/.
PMSD: 9.6 Upper PMSD bound determined by USEPA (90th percentile) = 350/.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimcphales growth in chionic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R4-0-003. US Enavironincitaj Protection Agency, Cincinnati, OIl.
Environmental Testing Solutions, LLC Statistical Analyses Stat*D Latyl PS& rmwIa" SarYWuTal-? DayGfOw~k
= 2 PPFRC
&ed Dow Y=.e Sum* , TVASWK I
Lab In: OuCM 101.- UV Treas
&Wq1eu M&S-v. TutalnSotueorw PtOcOL. EPAF 91-EPA Fruswatw SasIl"Type: M~~a4XwuepM-Uimiqg Ppo Tai Sped= PP-"ieaep Mogid t D,.Couwze 0.5253 0.4760
)55 0.4715 0432*7 0.43Z7 M0.98 0.4934 05203 0,5592 0.5839 22 0.4873 0.4553 0.4858 0.6213 43.9 0.7163 0.4926 0.5104 0.5423 72 0.6106 0.5 0.4654 0.5032 t0o 0.5014 0.53109 0.5160 0.5953 COM-% Mma N-Mem D- rol I Man Mb ThuifwmUasmj9,~
.~
.r;73;;
Z;: ;.Z 40z4453Ma-- NT*41-' a -Tailed Iooi "em 7.971 4 CziIwal MSD Mean 10.98 0.5392 1.1319 N-M a 0392 0.4934 0.5039 0-5273 22 0.5124 7.459 4 1.60-0 1.0757 0.5124 -1.365 2.410 0.1109 43.9 0.4553 0.6213 14.454 0.5273 1.0000 0.5654 1.1869 0.5654 4 0.783 0.4926 0.7163 2.410 0.1109 0.5273 72 0.5395 1.1325 18.161 4 -1.934 1.0000 0.5395 0.4654 0.6106 2.410 0.1109 0.5273 100 0.5309 12.392 4 -1.371 1.0000
.1144 0.5309 0.5034 2.410 0.1109 0.59531 8.149 4 0.5273 1.0000
-1.183 2.4t0 0.1109 0.5273 3.0000 AaSItlatyrTeats A-dfa Tau Shs Teat indi -e1a vf'ists] o(p 0.50) "h----i----'t-ca Skew Knr 0.9129 209"0.884 1.033702897
- 0 Dyoleu el(-al.00)NE 4.345994949 OC CV TU MSI~u MSDp 15.08631706 Dunne~sTest MSB MSE F-Prob
-00 f
>100 l 0.1t094044 02.32884626
& 0.003655427 0.004230141 0.5242ý3785 5.15 Point SD 95% CLExp)
IC05 ~>100 SkLte-Se be-w)
ICIO >100 IC15
>100 IC20 >100 IC25 1.0
>1300 IC40 089.
>1300 0.8 ICSO >1>00 0.7 0.6 S0.5 S0.4 o 0.3 w 0.2 0.1 0.0,
-0.2
-0.1
-0.3 L. - .
0 20 40 60 80 100 120 Dose %
0.8 0.7 0.6 0.5 0 0.4 0.8 . . .. . . . . . .
,N . . . . 1-tail, 0.05 level o0.3 of significance 0.2 0.1 0
sqnO2-26-02.xls
Environmental Testing Solutions, LLC Statistical Analyses
.d Dam et 2w /2M~
3/5M, a D 9RJ IhGownh Lra amd Sorvraj Tal-7 Day Growth a Esn.
M aw Torm, Sohwow Satipte m.
Sml Datm Sa.IPl TPK LVA bSQN. I w a UV T-rused Protocol EPA 91-EPA Preahwater D:- aS oW M-iin Pp.
Ten Speies: PP-lephl-Comm-% 1 2 3 4 ies poo*asa.
D-Cosmu 0.5253 0.4760 0.4715 0.4327 100 0.5579 0.5865 0.5405 0.5216 Cone-% Mean N-Mean* Tranform: Utransonred Max i-Tailed D-Cos'ol % N t-Stat 0.4764 1.0000 0.4764 Critical MSD 100 0-5516 0.4327 0.5253 7.971 1.1580 0_5516 0.5216 4 0.5865 4.999 4 -3207 1.943 0.0456 Auxiliary Tests ShapioF-Wi 'sTest e indicates uoa r eks al 0 .61 Statistic
.O1) Critical 0.965459824 Skew Kart FyTestindicatesequTevaraues (p( 0.749 0.61 0.314736868 -0.404384508 1.89620471 47.46834564 Hypothesis Test (Iti.0.85)
Hornoscedassc t Test indicates so signficast differeces Dose-Respoose Plot 0.7 1-tail, 0.05 level o.0.4 of significance 0
S0.3 sqn_02-26-02.xrls
Species: Ceriodaphniadubia and Pimephalespromelas Environmental Testing Solutions, LLC Summary of Chemical Analyses Client: Seqa oyah Nuclear Plant Non-Treated Test dates: Febr uary 26-March 05, 2002 Project number: 269 Pimephalespromelas C oldahnida b"k V.J1 0.2 Z*.31 24.2 2 "IU 4
'I, 'V 0.I 7.907 8.101 8.021 0.08
.17T 24.3 25.j 2.8! 0.3 244J 5.3 24.81
- 24. 2471 4.! 0 2421 4'1" 2441 Overall Temperature (*C) - . 25.2 24.6 0.2 24.2 25.3 26Q0 4+6 41.2 (including all concentrations for initial and final tempcraturcs) C4,?- o*,03-d2-
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPAI600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Daily Chemical Analyses Client: Sequoni ah Nuclear Plant Non-Treated Test dates: Februa ry 26-March 05, 2002 Project number: 269 Concenrat i on 1
1 arameter Initial uay u I Final I
Day I1 I iaul I Initial Day 2 I I Iybu I 1.,ial N I
. .. &I I
- 081 7.861 8.09 *111 7.93 1+S011m ,
IPH (bU)(mg/L) 8.0 7.5 7.6 L21 7.51 8.0 7.6 Control DO Codutivity Ot mhol/em)
Alkalinity (mg/L CaCO.O I
Hardness (mg/L CaCO%)
86
)!! (S)48.-
Temperature (C) 24.41 14.41 24.3 24 7 9d
- I 94 +* 94 9 Of1(SUi) Z. t.247 20 23 27 246I 243 24 2 2432 8.111 7.98 8.08 7.9 8.12 7.90 8.10 7.56 8.091 7.52 8.11 7.931 8.10 8.00 DO (mg1L) 8.0 7.9 80 74 7.8 7.7 7.9 7.1 8.1 6.5 8.1 10.98% Cnnduclivltz (Jsmboalcns) 7.5 8.0 7.4, 309. 32.2 304 308 302 304 310~
Temperature CC) 24.6 24.4 24.8 247 25.0 24.3 24.9 24.6 24.5 24.2 24.4 24.4 24.7 24.2 PH (SUJ) 8.11 7.95 77 8.07 12 7.90 81 7.57 8.06 70u 8.07 7.91 8.09 8.00 22% DOO(mgIL) 8.0 7.9 8.0 7.491 7.71 8.0 7.2 8.2 6.4 8.2 7.5 8.0 7A4 Conductivity (jsmhos/cm) 297 307 290 294 289 292 29z
________Temperature CC) 24.7 24.4 24.81 24.7 24.9 243.29 24.61 24.5 24.3 24.4 24.4 24.7 24.2 PH (SUI) 8.08 7.92 8.031 7.77 8.07 78 8.07 7.561 7.98 7.49 8.00 7.901 8.07 7.95__
43.% O (nsgL) 8.0 7.9 8.0 7.3 8.0 77 8.0 72 8.2 6.5 8.3 7.51 8.0 7.5 Conductivity (jsmbos/crn) 26.4 272 6 263 260 261 270
_________Temperature (C) 24.7 24.4 25.01 24.7 25.0 2 24.9 24.6 24.5242 24.5 24.41 24.8 24.2 PH (SU) 8m ~ .c n 1.70
~ ~ o, o 1,*0 .
fi.UL I "J'* I
...IKR 7Q(I T46 1 3.031 7.95 i
.,
- 0.*1.) I *r FIp D [%'*I DO (MgtL) 03 8.11 786 7991 7 803 802 788 790 72% 7.9 8.0 8.1 8.2 8.41 6.41 8.51 7.61 .9.0 7T Conductivity (Umhol/cm) 222 225 220 223 224 222 228ý
._+5.__ I I A J l Temperatre CC) 24.81 24.4 25.0 25.1 25.0 24.6 24.5
.4 PH (SU) 7.971 7.80 7.92 7.95 7.951 7.48 7.77 DO (mg/L) 8.3 7.7 8.2 8.2 8.3 7.4 8.5 Conductivity (,imbos/cm) 177 178 183 100% Alkalinity (mg/L CaCO%) 55 54 56 Hardness (mg/L CaCO,) 66 70 rotal Residual Chlorine (mg/L) <0.10 Temperature (CC) 25.01 24.41 25.21 24.71 25.21 24.3 25.01 24.61 I. 24.5 pH (SU) 7.961 8.01 7.881 7.711 7.921 7.771 7.911 7. 7.76 DO (mg/L) 8.31 7.81 8.2 7.51 9.21 7.7 8.4 8.5 Conductivity (Jsmhodcm) 177 176 I 175 100% intake Alkalinity (mg/L CaCOJ) 5
- - " I+- 59 Hardness (mg/L CaCO3 ) 6 76 Total Residual Chlorine (mg/L) <0 10 Temperature C'C) <0.10 25.21 24.4 24.9 24. 24.91 24.3 25.1 24.3 ýý 24.2
(*C) I Jemperature
ronmental Testing Solutions, LLC Species: Pimephalespromelas
&Od~oru- - CxApti. t-L 0 Date: nz-Zzf,-0.7 Client: "*JA S.
Full-strengthChemishry:
Control Batch:
Parameter S le Number:
3 0?-If-Oz 0,2,7 I PH 1
- 7.1 T2 T'.09 (s.u.)
DO 364 Conductivity (11 10 JM (r hos/cm) 641 Alkalinity S.5
- i5ka (ma CaCO3 IL)
Hardness (, -10 (mg CaCOJ/L)
Chlorine <0.0 -O L, <.0.10
(
(mfL*)
Collection start date Collection end time lqiI* Oct7G 1.
Grab or Composite -Zs-fJL_ 73-.+4o .o.
(durain KWAMO
((P*lr UAMM O4*r Temperature C°C) ciC 0.Zc 0.(
upon receipt 0qC 02C .b'.
Physical eAL. f characteristics 'euua W 4eu, CUeV- c+/-.$j Dates sample used - Z Z.& o'o3 -1 ESProject and 210 07.02%t,0I 42oOz, Sapenumbers
- 10 05 10 t.J N
a
- S - --
'3 1** -9 Iva; 0
1W 0 t~j e3 r~rjJ C I
vt 40 VI 1 V) 0 0
I, Nj 1 Z 0 ~ -9 -9 Zip-~J
'I
- r: -y
~ ~ zoti I
- - - - - 4 U 1 0 '9J
- a- - - - - - -
I.. o* G 0
A~ i '
0V I
0.-Q u uSu rA 1 - - - - - -O - - -
C U
vironmental Testing Solutions, LLC Species: Pimephalespromelas
- e AJ Date: OZ-Z(.O27_.
Client: "'A
Environmental Testing Solutionsa TjrC Page 8so ~s Species: Pimephalespromelas Client: -WA SgMWAAP POtjowf-eA-reb Date: O-.-z.
Concentrton Parameter3456 CONTROL pH(S.U.) 74-10 tWo f-1 to1 .41 11-1 6. 1 7I.V .i ý 7 DO' Cond8uct1ivit1y75
-t.0 "T111 77A Conductivity Temperature ~ ~ ~ ~ Z. qq 2~ q 4.
pH (S.U.) 9.10 7IS~
.80 *-z .o 7I ~ .o DO mgL 71 7.1. k. i. .5I i/ r Conductivity 3 33 (mos/cm) Zg 1
Temperature 24A~ INJ. Zq5 4,2
(-C) 24. 2, DO ýmg/) 8.0 t. 7. 8.(oAP R 7Ur. Conductivity Temperature Mq
__0c)__24.1C Zq. Zq~S ~qq DO (mg/L) 7,4 R-0. 1
- S.% Conductivity V4 Temperature 7sqc) q.co -,4SV. Z- 2t4.4 pH (S.U.) 9,0 'I.qS -J.-ef P4,, I-- a7.0 DO (mg/) z -
-. V Temperature 2-- 24.1 Z1 /( C. Z,~
V.
______p (SU) 1 l 79 intilI Final j nta IFinal IInitial IFinal I Tný*4 S4 11II Vlnq.I
=** I U
Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphniadubia Daily Chemical Analyses Client: Sequoyah Nuclear Plant Non-Treated Test dates: February 26-March 05, 2002 Project number: 269 Rev1ewed by; Concentration Parameter I Day 0 D ay I Day 2 Day 3 Day 4 Day S Day 6 pH (SU) 8.09 8.11 8.08 8.17 8.09 8.08 8.10 8.07 8.10 8.08 8.11 8.16 8.11 8.15 DO (ag/L) 8.0 8.1 8.0 8.2 7.6 8.0 7.9 8.1 8.0 8.1 8 8.0 8.0 8.1 Coondlt0 lvi- (IA m 2
tit 1 2m
- vu*N* v Jij, lima Control Alkalinity (mg/L CaCO 3) 65 I.
Hardness (mg/L CaCO3 ) 86 emperature (*C)
T_______ 24.3 24.5 24.6 24. 24.7 24.2 1 24.61 24.51 24.31 24.61 24.3 24524.71 24.3 PH (So) 8.11 8.14 8.08 8.8 8.12 8._0 8.10 8.09 8.091 8.07 8.11 8.78.10 8.17 109% DO (mig/L) 8.0 8.1 8.0 82 7.8 8.0 7.9 8.1 8.1 8.2 8.1 808.0 8.0 109% Conductivity (pmlsos/cm) 309 322 304 308 302 304 310 Temperature (*C) 24.5 24.5 24.7 244 24.9 ;24.2 24.7 24.5 24.5 24.6 24. 45 24.7 24.3 PH (SU) 8.11. 8.13 8.07 .9 8.12 8.9 8.10 8.09 8.06 8.07 8.07 8.48.09 8.14 DO (mngL) 8.0 8.1 8.0 8.2 7.9 8.0 8.0, 8.1. 8.2, 8.2, 8.2 .08.0 8.0 22% Conductivity (jsmhos/cm) 297 307 290 294 289M 292 298 Temperature (*C) 24.5 24.5 24.7 24.4 24.9 24.2 24.7 24.512. 24.61 24.4 24.5 24.7 24.3 PH (SU) 8.08 8.12 8.03 8.20 8.07 8.12 8.07 8.0 7.981 8.06 8.00 8.14 8.07 8.14 4.% DO (mgfL) 8.0. 8.1, 8.0 8.2 8.0 8.2 8.0 828.2 8.2. 8.3 8.1 8.0 8.1 Conductivity (pmhos/cm) 264 272 261 263 260 21270
_______Temperature (C) 24.8 24.5 24.9 24.4 25.0 24.2 24.7 24.5 24.6 24.6 24.4 24.5 24.6 24.3 pH (SU 8.03 8.10 7.98 8.14 8.03 8.08 8.02 8.04 7.88 7.99 7.90 8.07 8.03 8.12 DO (mg/L) 8.1 8.2 8.0 8.3 8.1 8.3 8.2 8.3 8.4 8.0 8.5 8.2 8.0 8.1 72% Conductivity (jsmhos/cm) 222 _____ 225___ 22022 ___24_2222
________Temperature (C) 24.9 24.5 24.9 24.4 25.0 24.2 24.8 24.5 24.61 24.61 24.ý5 24.5 24.6 24.3 PH1(SU) 7.97 7.97 7.92 8.10 7.951 8.03 7.95 7.90 7.771 7.96 7.751 8.061 7.99 8.10 DO (mg/L) 8.31 8.2, 8.2 8.4 8.21 8.31 8.31 8.31 8.51 8.01 8.61.... 8.2 8.1 82 Conductivity (pmihos/cm) 177 100% Alkalinity (mg/L CaCO3 )56 Hardness (sng/L CaCO3 ) 66=
Total Residual Chlorine (mg/L) <0.1010 T_______'emperature(C) 24.91 24.51 25.01 24.41 25.0 24.21 24.7 24. 24.61 24.61 24.71 24.51 24.71 24.31 PH1(SU) 7.96 8.03 7.88 8.121 7.921 8.061 7.911 8.2 7.761 7.991 7.731 8.221 7.991 8.1 DO (mg/L) 8.31 8.21 8.21 8. 8.2 8.1 8.41 8. 8.51 8.01 . 8.6 4 -. .
Conductivity (pinkos/cm) 177 17517 100% Intake Alkalinity (mg/L C*CO3 ) 545 Hardness (mg/L CaCO.3) 68M7 Total Residual Chlorine (mg/L) <0.109
_________ emperature (C) 25.31 24.51 24.81 24.41 24.9 24.2 24.612.44 2. 24.91 - 24.51 24.61 24.3
Environmental Testinz Solutions. LLC Page 5 of 8 Environmental Testin! Solutions. LLC Pages Ct Species: Ceriodaphniadubia Client: -TiJA Q~ iJ a%4 Ai& F:PLL. 10 Date: OZ-ZG-O2.
tAb3rj1-eAreb Full-strengthChemistry:
Parameter Sample Number: Control Batch:___
__ __ _ _ __ _ 1 2 3 dZ-If-oz. 02.-AdtZ pH .O DO (mng/L)_ _ _
Conductivity .i-1e I (pimhos/cm) _ _ _ __ _
(mg CaCO3LQ__ __ c!.L Hardness (mg CaCO 3/L) 4.'., xrelb,10 -64 I?,.ýo
ý Chlorine 0 .0 -C.1 Collection start date Collection end time Grab or Composite 7S-tW'J'- ThI: 4Ct?.. Mra (duration) "evseorp- Coi4VSrr4 C0ib Temperature C0C) 0.91 "C 62 I: 0-(.%C.
upon receipt I________ ____
Physical eALe em (
characteristics '4E.LLO) -k % ea%
C~ejV. C-LeA4g C-LeA4.
Dates sample used 62x oz-'zft,0oi. s- o2-o Q.zZ1O0z qf-6k0L 03-13a2.
ETS Project and 261 2 Sample numbers 02M6JO.61I 020271.0all?103OZ.Ol
Environmental Testing Solutions, UC Envionmnta Soutins.LLCPage Tetiu 6of 8 Species: Ceriodaphniadiibia Date: (.Z*2 KomkeATe1b FullstrengthChemiviry:
Parameter Sample Number ___Control Batch:___
___________ 12 3 6ZiR.f-Q7 02.zG-OZ pH .A- 71 DO Conductivity 1I73l0 3114 (gmnhos/cm) _ _ _ _ _ _ _ _ _ _
Ailcalinity 5A19(96 (mg CaCO3VL) _ __ N_
_ _ _ __ 1__ _ _
H-ardiness
~(mg CaCO3/L) -760 Chlorine 0J Collection start date 07407 z(at 2ý2-?
Collection end time NM 0 q3-s i BUS Grab or Composite MR6 Z3.&o0J' t3-Si000 (duration) cowifrre. cojwre Co~ros Mre.
Temperature (TC) 0.1w C.
upon receipt_____
Physical eAc. eAC k~
characteristics '4eu..ow J04Wa~ 4iPLLUw
%:uArts*
Dates sample used OZ2.-- ovz..fo? O302O6Z ETS Project and Vol9 2G?/6 Sample numbers lmz&d OOwI.l 0 2.
Environmental Testing Solutions, LLC Page 7 ofr EnirnenalTstn Souin.LCPae7o Species: Ceriodaphniadubia Client: "TVA SedQcHoA Date: 7.,zi6,o2 Daily Chemistry:
-Da Concentration Parameter 0 CONTROL pH(S.U.) itCA.
Conductivity IMAS (gmhosknm) iSZ Temperature
¢Oc) ?q.3 -q* z b -q3 24.4 7-4.
z 24, 7 pH (S.U.) r. Is. 1 8.10 DO (rag/L) * ,L . ' . ".*;
Conductivity B j37 (jimhos/km)I Temperature CC) z2.1 q, v. A
- z p) (S.U.) 417. -.f 3 .0 DO (mg/L) **. O '*,1 .. *,
2Z* Conductivity (pmhos/cm) Z Temperature
¢c) zq ,5 *.s" ~~z4.1 z,' q= a.*
pH (S.U.) (*0° *.IZ . 8.03 g.2 d0o" 6 ,tT.
4:.q ' Conductivity Temperature ~i(
pH (S.U.) 8.f &0. 1 .0-3 o9. A &. O7 8.1z DO(plos/cm)
(mg/L) 9- 9 2 "1z'* Conductivity ( 1 PI,Temperature (0C)(oc U) 9 .0- 9q.10 '7-.11 S.q z(f.o 05.e DO (sgu.) S..7-q l'* .t 7.q o (Vixhos/cm) 21 2 Z
-1 Conductivity L1..1 (OC)
Temperature 2 7s. Z
/NT(ýunzhoskm) .. .::::: /(ýo DO (mg/L) z.2. ,. 8.
Conductivity Temperature
_c)_*._
_z__.(C) 2q 454q -Nq.z Initial H Final Initial Final Initial Final
Environmental Testine Solutions. LLC ftge 8 of 8 Environmental Testin! Solutions, LLC Page of 5 Species: Ceriodaphniadubia Client: 'TVA cSp&a'lf Date: 02- 26-0Z.
Concentration Parameter34 ;ý.t4 CONTROL pH (S.U.) 6.10 T.010I 6.06 13I4
.11 '81 -1 1.
DO 1.9~13; 8.o S-1 Conductivity HAS p (phS.U.) .0 ll Temperature 74 0 -1A. 4. 3 24.~ 6zu -2 . T4~5 741 '2q.3 pH (S.U.) 6-o R.04 0.0~-4 8.O11 .r .1 DO (mL 21.9 A - a -I2 1.2&.0 J).0 7 2 Conductivity t (jirhos/cm)
Temperature 7.4415 2q. 6~ 2q. 5 4 DO (mg/L) A'O97.,
27 Conductivity zl7 Temperature -zq.- zc4.5" 24A. 21.q _2q4, -z .Lf3 PH (S.U.) . . . 3 R.O 1.4 DO (mg/L) h.Z 0 . 9.70 6.3 e&0 A13.
q Conductivity ZO2(lZ1 (RP*uhS/CM) rZeb Temperature ~ ~ q~?.S 7qqS 2. 4 PH (S.U.) 6.10.. -1.1 00.6-1 &2.0 Ile.
DO (mg/L) ýP. .Z 8k5gZs M~c a&..-
0 Conductivity z Z z 2
/Ah~ (Wnhos/CM) 3 Temperature zq.Qo 2 q~ zzq.5 .2qz.5 'I 2'/.(~z
_____ ____ (0c)
Ibnfitfia IFal l IInitial I Final I Initial g Final I Initial 11 b"'u'i.
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Methods 1000.0)
Species: Pimephalespromelas Summary of Chemical Analyses Ckm: Slqaaya N.dta. PMlWUV-Truad Tm dlun: Fclooaq 26-iarch 05, 2002 Frojý -- 1-;b, 269 R.,,k-d b .. IA- .*i-PimephaLkspronw1a 0 24.21 24.7 24.41 0.2
.051 7.51 7.961 7.821 0.16 D.2 6.6 7.9 7.41 04 24.3 25.21 24.91 0.3 0 24.21 24.61 24.4 0.1 05 .50 7.94 7.81 0.16
)72 6.6 7.9 7.5 0.4 24.
"245 252 24.9 0.3 24.21 24.61 24.4 0.1 25755 291 7.77 015 Orall T-qaraat- CC) 24.2 25.5 24.7 0.2 (induding al c
- waoa iwtiaraol. ail ara*a) andp&W
Alida vaz uuiaxI.iital I eUstilg 3oWuElons, LLU Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Daily Chemical Analyses Client: Sequoyah Nuclear Plant UV-Treated Test dates: February 26-March 05, 2002 Project number: 269 Revieed by: _-*J _
Concentration Parameter D D D D 4 Conal Alktiat Cnitia 9i Iniia DO (ra./L) 8.5 8.0 8.0 77 82 6 Control Conductivity 7.6 7.9 7-5 Ofmpraure (phos/cm) 1 5843 249 8.C0 2.40 Alkalinity (mg/LCACO3 ) 47649 244 2 8.1 2496745 2494. 24.524. 24.3 Hardness (mg/L CaCO 3) 6 Temperature (1C)245 243 2442 .1 2 .1 2 .241 2 .1 PH (SU)8.280248 2 -1 2.3 2% Conductivity (jzmhos/cm)29 10.98% DO (rag!!, 8.0) .7781 6.1 - . .9 - .
Conductivity (pmhos/cm) 010
<0.10 Temperature (C) 3010 24.9 24.7 PH_*(su) -- 24.5 2.
25.0 244 2544 24449 22% DO (rag/L) 801 781 80 .1-f- .80 Conductivity (jumhos/em) 80 .1 Ro 7. . .
T'emperature (10 9029 pH (SU) 80 .4 .3 .8 80 .1 .8232.
4.% DO (mg/l,) 82 7 . . .579 43.% 8.0 7,a/L Conductivity ()Amhos/cm) 7 .
T empe rature (°C) 2 .1 2 .1 2 . 46 2 . 44 2 . 45 2 , 4 52 .
7 % DO (rag/L)8.8. 7. 8.T7 Conductivity 7873 - 83.680 76
-(pmihos/cm)2121222 Temperature (,C)2504A pll (SU) 79 DO (m g/ )8 .7 . 18 17 .. 47 88 078 . 17 17 67 Conductivity 0tmhos/cm)1701812 6. .
100%Itk Alkalinity (mig/L CaCO3) 5 Hardness (mg/L CaCO3) 6 Total Residual Chlorine (rag/L) <.1 Te mpe r a tu r e ( *C
) 2 .1 2 .1 2 . 5 42 .1 2 . 48 4 52 . 4 52 . 4
Environmental Testing Solutions, LLC Page 5 of 8 Environmental Testing Solutions, LLC Page 5 018 Species: Pitephalespromelas client: -TIA- SeQ uo-YA 14 - O,.n-pAw 101 Date: 6i2- 2~-(z (1' - TreATeb Full-strengthChtemistiy:
Parameter Sale Nuber: Control Batch:_ __
__________ 1 2 3 O-~O. 2&3 pH -. fs (S.U.) 1A5 I_ _ __.
DO -[A (In'g/L)__ _ _ _ _ _ __ _ _ _ _ _
Conductivity 11 C ? -0 (Vimhos/cm)__ _ _ _ _ _ Z5O AkAlinity S , 1 16 (mg CaCO 3 /L) _ I_ _ _ _ _ _ _ _ _
Hardness 170 10 (mR CaCOJL)
Chlorine0.1 Collection start date Z202 2-. z 2L207 Collection end time 14S O~e 15 Grab or Composite 25-810WC 2J$.$OiVe Za3kotz (duration) catousre. ComjPOrrd ca$PsMl Temperature CC) 0..'(1 le 010 06C.
upon receipt ____ ________
characteristics 'jCLLuý *L" cLLeA e- CiAA CLeA. ______
Dates sample used 07,;.&Oz. 0oi-*al 03-2-Zo ETS Project and 1Sample numbers LOZCoZ .61 lozOZt. 0 (302050Z.OI
Environmenta Testing Solutions, LLC Page 6 ot 8 Species: Pimepialespromelas Client: 7 -ed
" aq A - INTA*e Date: Z-oz.
UV/- r9e.'reb Full-strengthChemistry:
Parameter S De Number:
S1 Control Batch:
2 3 pH s.u.) -7.q , 1,9 .*.co 0ot~,
DO Batch*.:....
(m9) 9. ,.5 o6:5o*
Conductivity (gmhos/cn) 1-7 1-78 Alkalinity (mg CaCOL/L)
Hardness .* 59
-t Chlorine I0I .( .~
Collection start date 10'- zq -o7 o2 -z* .*Z zo-d -o2 O _.9 Collection end time Gr-ab or C ýomposite 25-14bat 23 AW*L "as.*.ovo?.
( du r a ti on )
- O* T . .. ' ; "
Temperature C°C) upon receipt O.606C (6.. I.C, Physical eAL- AIO, ALe characteristics leaa Aeu-o *t*
"u Dates sample used - o.Z.-oZ 3.O1-oz-V.o-oz 6o6.,-Z 02-ZI2 4;-"Z.
. OMq-OZ I
ETS Project and Sample numbers Z
a20zoZ.
U01 damrzzf.07. MOO2W
Environmental Testing Solutions, LLC Page 7 of 8 Species: Piniephalespromelas Client: TA - fqi~e.q0AA4 LNV - eAeb Date: ~-2ad Concentration IParameter CONTROL -PH (S.U.)
ConductiVit
.(limos/cm)
Temperaturme
("C) pH (S.U)
-DO (mFOL)
Conductivity
_ýýOs/cxn)
Temperature C)
PH (S.U.) ?4.3 ci
.c--Zq
,DO (mg/L)
Conductivity
("3hos/crn)
Temperature C
pH (S DO ( rig/L)
Conductivity os/cm)
Temperature (0c) 4'3 2q.2 W~.0
_pH (S DO (mg/L) 72 %, Conductivity J 0S/CM)
Temperature CC) pH (S.U.)
DO (mg/L Conductivity
/a)Tal DS/CM)
Temperature 0C
-PH (S.U.)
DO (mg/L) R.. z Conductivity 16001"K ( )S/cm) J'X Temperature (0c) 2r. 0 Initial I Final Initial 11 M_ I Inital FnalI I Th~i1It .5~A~ III la
.i7ulu Inam - I
Enviroamental Testing Solutions, LLC EnvionmetalTestn!
Slutons.LLCPa~e s atf s Species: Pimtephales promeloi Client: -NJA-GeagcH-At4 Date: 02-Z6-07.
Da Concentration Parameter 3...
CONTROL pf S.U) 0.1 1 4G. . eoJ7 DO (mg(L) -7, 1. .1 f31 to 7.4 Conductivity _ 13 Temperature ~ ~ , 2 1 ~
DO (mg/L) 1.71.
Z20. Conductivity Temperature 71.
CC)O ZLS 21.1R~ -q 4'7 zq. zq457 14.
pH (S.U.) i.1o
-7Os AS.1 8.
2 lConductivity~ghsc)Z9L Temperature Zsl N ~. 2.O U 8 4
_ _ _ _ CC) z
( mgC DO____ )-79' (4 Conductivity (Ipmhos/m 7)0 0(L 2-c Temperature ~2. ~C
_ (S.C) pH__ 20.0- -7.(.t 12ý,F Conductivity I Mr-~. (jnuhos/cm) 2 4z Temperature 2. . 4 ~ . ~ q VZ
(
DO________ 3 Inta I Finl I Initil Final I nta I I'ni nta I Final
Enrvironmental Testing Solutions, LLC Page to5 PageIof Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accunmet Model 25 pH/Ion Meter Iodide reagent Date analyzed -Ztp4t Acid reagent:
Slope:
Calibration:
0.10 r/L 1.00msh Reference standard number IAS 0,IA Note:~For samples with a residual chlorine of > 1.0 ing/l, the calibration range must be adjusted to bracket the chlorine levels of the sample..
Laboratorycontrolstandard:
Reference standard True value (TV) Measured value (MV) %RS=MV/TVx 100 number (mng(L) (mgiL) (acceptable range - 90 to 110%)
I 0.50 o0 IZ Duplicatesample precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) /[(S+D)/2]} x 100 number m (acceptable range - 10%)
4/ Duplicate 11111 M D4~o L Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine inumnber (1119P number Blank (should be=<0. 10mg/ýL) _____
Note: All samples were analyzed in excess of EPA recommended holding time (l5 minutes) unless otherwise noted.
Laboratorycontrol standard:
Reference standard True value (TV) Measured value (MV) %/RSP=MV/TVx100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)
IN os 0 0.50 0,eu 10Z.19%
Reviewed by I Date reviewed I LN4;1.-OZ
Environmtental Testing Solutions, LLC Page (^o Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter. Accumet Model 25 pH/Ion Meter Iodide reagent:
Date analyzed 0-1.29.0Z Acid reagent:
Slope:
Calibration:
- .- [ - .- . 0.10 MR/L 1.00 mg/L Reference standard number > . 0 h6ms tOo b Note: For samples with a residual chlorine of > 1.0 mg/I-. the calibration range must be adjusted to bracke the chlorine levels ofthe samples.
Laboratorycontrol standard:
Reference standard True value (TV) Measured value (MV) %RS=MV/TVx 100 number ((mV/L) (mgL) (acceptable range - 90 to 110%)
0.50 IO'I. q Duplicatesample precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) /[(S+D)/2]} x 100 number (m1/L) (acceptable rage 10%)
.OW222t.O1 SQ, OiTFALL IC I OI O jlrUej*ft_, S ,0.0 7l DuplicateDID. 20 Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number mmbeL_
s1 Blank (shouldbe=<O.lOmg/L)F OOO-..
4D.4 o~o2Zf. O2Z S- J iNr-AKe 9 oJL.C es *.U LO cWLO Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otheswise noted.
Laboratory control standard:
Reference standard True value (TV) Measured value (hMV % RS =MV/TVi 100 number (sng(L) (sng/L) (acceptable range -90 to 110%/)
)r OU60.50 c.0-54q Reviewed by Date reviewed
Environmental Testing Solutions, LLC Page ("3 Page tL of LI Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter- Accumet Model 25 pH/Ion Meter Analyst L .- Z. Iodide reagent:
Date analyzed [p)5-Oj3J Acid reagent:
Slope:
Calibration:
R EM M M o.1o ;= 1.oo mR/
Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusto bracket the chlorine levels of the samples.
Laboratorycontrolstandard:
Reference standard True value (TV) Measured value (MV) % RS = MV /ITV 100 number (mg(L) (me/L) (acceptable range -90 to 110%)
I MAx.SooS 0.50 o. 0 q3. rib Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine numb er (m*/L)
Blank (shouldbe=<0.10mg/L) 7-.0,0096e5 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory con trot standard:
Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range - 90 to 110%)
IA 6o00o5 0.50 o .'1eI q( .7,v77t Reviewed by 4hI II Date reviewed IQ-oaL2
Environmental Testing Sohlutions_ .r.t Page L4I
.... . .. . ' -- Page I _of _
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Date analyzed [),5iU5_ Titrate samples to pH = 4.50 S.U.
ýraxt nonnality and mudltipie deter, pH of Delonized water
-4.5 S.U.
(.-4
, - eotteut 0.1 - o.2 -o.*f*
oratorycontrolstandard.
I Reference True value Sample standard number (TV) volume Begin (Mcg CO (nil) m I 60.-o4,. 100 100 17.5 Duplicate sanwile nrecisi,..
Sample Alkalinity.
Sample Sample ID volume %RPD Begin End Total Multiplier (mg CaCO,/L) {(S - D) /[(S+D)2]} 100 number ml ml ml ml (acceptable range = 1o%)
J Oz-z.0o. 0Ht5-b8o H7,0 I, .. .** D(
LZ-zL,-oZ pct li *
- Z9.C 3Dp Oe D LaO.o Matrix spike recoverv:
Reference I Spike value I Sample i I I I i standard number (SV) volume Begin End Total Multiplier (mg CaCOaiL)
CaCO 3/L mL Zq,ml ml ml
- 61) - ~ . ~~4 10029q .1 1 'Tq [1 Sample alkalinity (B) Measured spike value (MV) % R = MVI/ SV x 100 (mng CaCO3/L) MV = A - B (acceptable range
(-g CaCO3fT..- 75 to 125%)
41 _0__ ___4_ _
Sample measuremen ts:,m I I Sample volume Begin Total Sample number End Multiplier Alkalinity Sample ID (ml) ml ml ml q.fl Multiplier (me CaCO3JL)
-,s ,l* 2o 140.1 to -z*.1-0 Z 0.0 03 -o i -O. 6SU. HIo (o OZO 224a. 0 1 3.0 So 0zo ZZ(5, 0) ZI.D 55 OZ0302.o Z(.W 3*.ze 5-S 0 z 0 zzg. U-. 6.0 l
- 1. 3*,..
0200,,04,01- 5Lq I ],m- I 7' Reviewed I I I~~w U6-04-0t- ryl tt.5 Review ed I Date rete ed Date reviewed: -cx5--a?.
Page LIZ Environmental Testing Solutions, LLC Page Z~ of Z Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO 3/L Anls Date analyzed Titrate samples to pH = 4.50 S.U.
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)
TV~3..uz* Iq6~cq~oZ 0 .5 _ ._
3 _ .q
"_3 few..e Dtre: 3.9d0 31, s** 0 -,.
o 3 -7,. -+
":t 1, t.3,)I .3 zq-C)
Reviewed by: Date reviewed: I ,;ý" 05" 07
Page Lof t Eknviron'mental Testing Solutions, LLC Total Hardness (EPA Method 130.2)
Matrix: Water, MIDL =1.0 mg CaCO 3/L Analyst p Date analyzed Titrant normalitV and multiplier detemnatin:
pH Factor or Mu~ltipliler Normality (N) of XDTA
=0.21E .(N x 5M00)I0ml sample rerence standard mil ml End ml Total cheeck LBeegin (acIal age01-OOl - NI 000 Titrant number N~ormality number 0- OW zo
_C)~IT 0 .0 10-0 lo.0 Laboratory control satadarik -
I1arwdnems (MWV) I %RS =MV /TV x 100 Reference True value Sample standard number (TV) volume Begin End Total m Multipler (I g CaCO/L) (acceptable range
- 90 to 110%)
(in_ Ca)OmL ) ml ml 40 50 1o.0 1,.0 .
Du icafe sam levrecision: =
Hardness "%PD Sample End Total Multiplier (mg CaCO/) {(S - D) It(S+D)r211 x 100 Sample Sample ID volume Begin (eptable
- range-< : 10%) ,
mber number (m) Int ml m
[.o L D*
S IV 02.2o.OZ? Dpicate1-1?O Duplicate ~
Matrix spike recoveryV: SiehrnsA A Siehrns Reference I Spike value ISample mCCaL number (SV) volume Begin Ed TtlMlile standard ml ml mCaCO3/L ml ml
=MV / I S x10 M ) % R cceptable ra:nge value A-_B (a)
(gCi/)MV= Measured spikeCaJuL to 125%)
Sample hardness (B) =75 ccm
Page
- of "
Environmental Testing Solutions, LLC Total Hardness t'e (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst A Date analyzed o.s Tifantand mfi~erd&ermh~atlof: pH Factor or Multiplier Begin End Total Normality (N) of EDTA
........ (N I SM 000Y50m l sam....ple number (wmeptab" range-1 - 0.022)
-0.2/ 0.018 number ml nil ml fe m bere1n uebta st-aand aer
[Lnr rence Laborato-- control s-anda Hardness (MV) %.RS=MVTVx100 Reference True value Sample (mg CaCO-) (aptable r Begin nd Total Multiplier standard number (Tvolume 90"to.110/6)
(mg CaCO/)
40 50 Date reviewed Note: If > 15m of titrant is used, sample must be diluted. Reviewed. by:"
Sequoyah Nuclear Plant Biomonitoring February 26 - March 05, 2002 Appendix D Reference Toxicant Test and Control Chart Information
MAR,08.2002 10:48 8288628195 ENVIRONMENTAL TESTING SOLUTIONS #1274 P.033/037 Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water Test number 'r'est date 7-day TC25 CT S '13&7 CV (g KCI/I.) (g*L KCI) 1 03-17-00 0,68 2 05-23-00 0.69 0.68 0.01 0.26 0.31 0.01 3 06-13-00 0.72 0.70 0.03 0.26 0.31 0.04 4 06-13-00 0.70 0.70 0.02 0.26 0.31 0.03 5 09-19-00 0.66 0.69 0.03 0.26 0.31 0.04 6 10-24-00 0.77 0.70 0.04 0.27 0.32 0.06 7 11-07-00 0.65 0.70 0.04 0.26 0.31 0.06 8 03-13-01 0,69 0.69 0.04 0.26 0.31 0.05 9 06-26-01 0.53 0.68 0.06 0.26 10 0.30 0.09 07-17-01 0.74 0.68 0.06 0.26 0.31 0.09 11 08-21-01 0.62 0.68 0.06 0.26 0.30 0.09 12 09-25-01 0.60 0.67 0.06 0,25 0.30 0.10 13 11-01-0] 0.71 0.67 0.06 0.26 0.30 0.09 14 11-06-01 0.66 0.67 0.06 0.26 0.30 0.09 15 11-27-01 0.57 0.67 0.06 0.25 0.30 0.10 16 12-12-01 0.63 0.66 0.06 0.25 0.30 0.09 17 01-04-02 0.49 0.65 0.07 0.25 0.29 0.11 18 02-05-02 0.61 0.65 0.07 0,25 0.29 0.11 19 02-13-02 0.65 0.65 0.07 0.25 0.29 0.11 20 02.26-02 0,56 0.65 0.07 0.25 0.29 0.11 Note: 7-d IC25 day 25% inhibition concentration. An estimation of the concentration ol potassium chloride that would cause a 25% reduction in Pirnephales growth for the test population.
CT = Central tendency (mean IC25).
S = Standard deviation of the 1C25 values.
S.1 - Standard deviation corresponding to the the 7 5 th percentile CV.
SA.75 - 0.38, as dIcturmincd by USEPA for the mcthoxd znd e.ndpoint.
S.,_0- Standard deviation corresponding to the the 90"' percentile CV.
SA.90-- 0.45, as determined by the USEPA for the method and endpoint, CV = Coefficient of variation of the TC25 values.
USEPA. 2000. Undet-randing and Ao;Lumnting for Method Variability inWhole Effluent Toxicity Applications Under the National PoluLantL Di,4chury Elimination Program. EI'A-833-R.-b-0(03. US Envinronmental Protection Agency, Cincinntati, OH.
Organisms obhuinedfrom Aquatic BioSysiems, Inc.
02-26-02.--k MAR-08-2002 10:00 8288628195 P.033
Environmental Testing Solutions, LLC 0
I I0 f-,
Potassium Chloride Chronic Reference Toxicant Control Chart to 0
for Pimephalespromelas CD
- 0. using Moderately Hard Synthetic Water 0
1.2 1.0 cO ro U
03 0.8 NJ b1 01 In U 0.6 V
N 0.4 0.2 41=
Test date to
-J I.
'a 7-&y IC25 = 25% inhibition concentration. An estimation ofthe concentration of potassium chloride fltat would cause a 25% reduction in Phnephalesgrowth for L'J 01 the test population.
S-- - Central Tendency (mean IC25)
Control Limits (mean I125 + 2 Standard Deviations)
MAR.UaO.002 Ia:49 U288628195 ENVIRONMENTAL TESTING SOLUTIONS #1274 P.036/037 Environmental Testing Solutions, LLC Precision of Endpotnt Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromelas using Moderately Hard Synthetic Water Test number Test date Control Control Mean Survival Growth C:V CT MSD PMSD Cr C/o) (rng/Awva.) C/c) wturCnu(%i (0/) for PkMSV (/)
Growal CV 0~/.)
I 03-17-00 97.5 0.40 7.5 2 0.06 15.7 05-23-00 100 0.31 3.6 5.5 0.07 21.2 1X.4 3 06-13-00 100 0.45 10.1 7.1 0.10 22.2 19.7 4 06-13-00 100 0,58 13.2 8.6 0.12 20.0 19.7 5 09-19-00 t00 0.67 5.3 7.9 0.07 11.2 18.0 6 10-24-00 97.5 0.83 13.3 8.8 0,22 26,9 19.5 7 11-07-41 97,5 0.67 10.5 0.13 19.3 19.5 8 03-13-01 92.5 0.34 9.1 6.5 0.08 22.8 9 06-26-01 19.9 100 0.63 9.8 8.8 8.9 0,19 10 07-17-01 30.6 21.1 100 0.52 9.1 8.9 11 0.07 13.8 20.4 08-21-01 100 0.89 8.3 8.8 0.13 15.1 19.9 12 09-25-01 100 0.85 4,9 8.5 0.09 10.4 13 11-01-01 19.1 100 0.54 2.5 8.0 0.10 18,6 19.1 14 11-06-01 100 1.00 7A4 8.0 0.17 16.9 18.9 15 11-27-01 97.5 0.70 22.8 9,0 26.1 16 12-12-01 95 19.4 0.82 16.6 9.5 0.19 23.5 17 01-04-02 19.6 95 0.80 10.t 0.18 18 9,5 22.0 19.8 02-05-02 97.5 0.95 6.9 0.21 9.4 22.2 19.9 19 02-13-02 I00 0.65 R.o 0.10 9.3 15.5 19.7 20 02-26-02 100 0.64 3.3 9.0 0.11 16.5 19.5 Now: CV = Coefficient on.rvjrtion for contfrl survival.
On average, the CV for control growth i 9.0. in Envimv~uncal "lnting Solutions, 1.112Pinwpholcc chronic toxicity tet;.
tovei CV boutmd determined by JSEPA (IC"' .lerentile))
-3..*%
Upper CV bound dotennired by USIIPA ( n pereendtile)=
90 20%
MSD = Minimum Sigdaticat Ditfereoea P.MSD - Percent Minnmnm Significaat Difference PNSI) is a Incasure Of test precision. The PMSD in the mininiuns percent difflcnce b*tweea the contwl and treament that caa be declared statistically significamt in a wbol ct-llueat toxicity test. Ot average. a significant difference occurs for Envir*nmentl t'esfing Solutions, 1.14'Chronic toxicity tests when a toxicant reduces Ptmephaltngrtwit by 1945% Brom the cunroL owerlPMSD bound determined by UJSýPA (I0' pcrceintila)
- 9,4%.,
Upper PMSn bound dtcetmined hy (ISF.PA (90QW pm.-tntile) - 35%.
CT' = Central 1'ndaney (mewn Control Growhfl CV Of le-m PMSD) lhe luoer and upper bo*nids were calcuLhted by the USEPA
.,hng 205 tew.sscondocted fromn 19 labonturie fur Pinwphaloes grwth in chrunic reference lOxiuitrt lesls.
IISEPA. 2000. Understanding and Acoantling for Mehod Variability in Whole F.Muettl Tox icity Applications Under Dischar .*. uutihimtun tl rogp-A-a.. the National Pollurnt HPA-i13-R.00-003. US lrnvironrnenrl Prnoetiot Ageny,'
Cinuinoiti, 0i.
Organcisns ori.'tauii firm Aquade llioVStreot, m:.
MRR-08-2302 10:01 8288628195 P. 036
Environmental Testing Solutions, LLC uI 0) to Precision of Endpoint Measurements C Potas;iu nm Chlorlde Chronic Referzace Toxicant Control Chart Ri N for Pimephalespromelas using Moderately Hard Synthetic Water 40 30 CO
'xi 00 PLJ CO k0) H (A.
S20 0CEn
'C H
r 10 C" o
CR 0
'0. 06 4 4? " k%% ý 0 ttt,
'O%-
01V Test d2te PMSD = percent minimum significant difference. PMISD is the minimumn significant difference between the control and treatment that can be declared statistically significant.
Y3 S- Central rendency (mean PMSD)
....... Lower and Upper PMSID Bounds Lower PMSD Bound (10i' percentile) = 9.4%. Upper PIVMSD Bound (90" percentile)
= 35%
(Lower and upper PMSD bounds were. determined by USEPA for the method and endpoint.)
-r 33 Environmental Testing Solutions, LLC 0l CD II 0 03 rII LA' ci Potassium Chloride Chronic Reference Toxicant Control Chart (U for Pinephalespromelas ciN using Moderately Hard Synthetic Water o° 2.0 I t I I I I I I I I I I I I i I I I Warningand ControlLimits Set According to 75IA and 90'4 PercentileCVs.
1.5 U ................ ...........- - ................ . . . ...... ................................................................................
co z 1.0 N
(A1 to U 0.5 N S.................................................. .. ................................................................
0.0 Since the coefficient ofvariation for the set of 7-d IC25 values (0.11) is less than the 75?I and 901 percentiles determircd by the USEPA, these percentiles were used in setting the warning and control limits.
I I I I l I I I I l I I I I I IOR OO Wt CT 4 <V Test date 0 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
- U
- - Central Tendency (mean IC25)
.ix 0t:
-... Warning Limits (mean IC25+/- SA75 SA."7 = 0.38, as determined by USEPA for the method and endpoint)
......... Control Limits (mean IC25 + SA.90, SA.% A = 0.45, as determined by USEPA for the method and endpoint)
miLif"I'U"U1 I'L,140*14148,1, JL -I.I.-
ENVIRONMENTAL TESTING SOLUTIONS #1274 P.024/037 Potassium Chloride Chronic Reference Toxicant Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephalespromdas PpKCICR Tcst Number: L3 Dilution prEpatdon inorm,.on: Comments:
KCI CM1M number C_., 0__,_
Stock preparation: 50 g KCl/L: Dissolve 50 &KC1 in 1-L Deionized watar Dibron prep (rag/L) 300 450 600 750 900 Stc9oii m 12 15 7 DilucMxvolmw (mL) 994 991 98t 985 982 Te-vt organi.m Information:.Tet infomta,,: _
Organism ag'e: 2'4- k0.9 0 &.t Randomizing template: __tt__
Date and times organisms 0-'2S.Qz I%'oo Tb K Incubator number:
were born between: _ _ __-_ -_ _.
Organism source: A S 6prTCI" o.-t., Artemia lot number: olp Transfer bowl information: pH = Tempcratae = Oven temperature- 1__000C
"-"2q,2C Drying time:
i.'...
Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Test Initiation, renewal. Analyst time time or ermination time o0 2Q. - A it19o _jqz? A
.02-71.0z JJ3f I . Iq 4 .q*..e doz 1'50 Si5 - . . M31 ..... 1 ..
tSqo l ' lI Control information,. "O°* Summary of te-t endpoints:.]
% Moitality: 7-day LC50i 7 '..
Average weight per larvae: 0,4 . 0,2.) mplarvac NOEC ....
LOEC
- TC25... %f q MAR-08-21302 09:58 8288628195 P. 024
ENVIRONMENTAL TESTING SOLUTIONS #1274 P.0251037 Environmental 'festi¶g olutions, LLLk I PpKCICR Test Numbcr: 13
______________ ________________Survival and Growth Data __________
DyCONTROL 300 m KCI/L 450 mg KCV/L 0_______ 10 to 0 /() /0 /0 /'0 /0'o /6 1 /6 ,a' I________ /A /D /0 /b12k Id 6 10 /0 /0 /0 / c 2 /t0/ to0to/0/16 /6 /6 c/X 10 14D ,c 10 (o 0 10A') to to t10 J 10 7 /c 10 10 10 /0 / 0 q / 0 /
A-Panweiiht(ftlg)
B-Pan +LtuFVUC weight ~zg) 4.li t- Lo~ tp Calculations wid data reviewed,:A Comnments:
MflR-08-2i302 09:58 8288628K95 P.025
MAR.O0J.2002 10:4b U288628195 ENVIRONMENTAL TESTING SOLUTIONS #1274 P.026/037 Environmental Testing bolutions, LLA-A PpKCICR Test Number: 13 Calculations and dau reviewe&
Comments:
MAR-08-2202 09:58 8288628195 P. 026
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.3
MAR.08.2002 10:46 8288628195 ENVIRONMENTAL TESTING SOLUTIONS #1274 P.027/037 E-nvironmental Testing Solutions, LLC Piage 4 o0 1
PpKCICR Tt Number: 13 MIS Control Chemistry:
Paramcter Control Batch:
_Z-__,-_ __-__ 07. \
pH (s.u.) at *o.O DO (mg/L) ,,,_ __ _
Conductivity Grnihos/cmi) -\____
Alkalinity (Mg CaC0WL) ____
Hardness (mgCaC0 ,L)
Ca_0 33_(_)
Acceptance Criteriafor Daily Chenistry:
Acceptance Criteria Concentration (diluti-m m..t be remade ifthe conductivity fals*otsido 1ho
_ _ ta*birangic)
CONTROL 280 - 360 300 mg KClIL 840 - 890 450 mg KCI/L 1100 - 1180 600 mg KCIIL 1390- 1460 750 mg KCI/L 1660-1710 900 mg KCI/L 1890 - 1950 KCI Stock 67000 - 76000 (25 g KCVL)
MAR-08-23082 09:58 82e8628195 P. 02?
4AK. Uts ;&Uv;ý LV : '110 UZU 8bz 8 19!) ENVIRONMENTAL TESTING SOLUTIONS #1274 P.028/037 F rj~vUV: uUZlZL~JIj 4.1.* lr.*.,*in
'w 1.9nivitinnt LL-PpKC1CR Test Number M__
Concentration Parameter...
CONTROL WpLý.U 8.C- 1.
DO (mg1L) '1.
300mgKC/LConductivity al
(~hos/cm)
Temperature 2q. -EA tv Tcmperur 4 Ll LI kp10(SAJ) ~ pzTh 18.' 7( ,
,g~ig/L) 600 mng KCI!L Conductivity (jnnbos/crn) 3 Temperature ~f3 w41.6J.
PH (S.U.) 4r Zc, 8.- e, 15 4.9e DO OWL) R.. I.(,
7,50 mug K-CIIL Conductivity 10 14 1 .
Temperature zq~ 2q5 T 2134.O S .5 ~O p(0CL)
DO (mgfL) ,
900 rug KCIIL Conductivity ,z' 4(j8.imhoscI Temperature 2qq .43 M4 -(.
CC)
IInitial Il Final _j (ntin]IIJt~a Inlitial 1 jinfai -
- 4 a0 107-0
-7200' MAR-o8-2Z302 09:59 88689 82eB628195 .2 P. 028
MAR.08.2002 10:47 82886281.95 ENVIRONMENTAL TESTING SOLUTIONS #1.274 P.0291037 P, Environmental Testing Solutions,LC Page 6 ot .5 PpKCICR Test Numib=r 15 1 T uja U Concentration Parameter p - I-., - I r'n 'ronr I.U P~.
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Conductivity (pimhos/crn) IZoe) '3)' 4313 Temperature (CC) 2r4,3:3 300 Mg KClIL Conductivity bo,6!
(ihs/cm)
Temperature *4 ~. Aa z, 4~215 2
- q. DO Lm.g/) 9-, 1. Wii. &i 450 mug KCIJL Conauctvity 1-
_0mos/cm) 1 1 Temperature ~~2. z. '.~7Q pH (S.U.)0 . r &.
6050 mg KCI/L COnutvy(mg/4 900 mgKCI/LConductivity (Mfos/cm) I~7I~
Temperature q 2-7
__ __ __ (0c) Z~S-
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Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphniadubia using Moderately Hard Synthetic Water Test Tesdat Control Control Mean nmber Survival Reproduction CV Cr MSD PMSD CTI for Control
(.) (oprine/femae) (%) Reprodton (%) for PMSD (%)
CV (%)
1 12-13-00 100 21.8 11.6 2.6 11.8 2 12-17-00 100 24.0 16.0 13.8 3.5 14.6 3 12-17-00 100 13.2 25.6 4.6 10.7 3.3 4 12-19-00 12.9 13.1 100 24.8 9.5 10.4 5 4.6 18.4 14.4 12-19-00 1oo 25.1 19.2 12.2 5.0 19.8 6 01-09-01 100 15.5 28.0 8.4 11.6 3.5 7 02-06-01 12.4 15.0 100 22.1 10.1 11.3 8 3.1 14.2 14.9 03-07-01 100 24.0 8.3 11.0 2.4 10.1 14.3 9 04-04-01 100 25.0 10.0 10.9 2.9 11.8 10 05-09-01 100 14.0 27.9 8.9 10.7 3.1 11 06-06-01 11.0 13.7 100 27.8 5.8 10.2 3.5 12.5 13.6 12 07-10-01 100 25.0 9.4 10.1 2.8 11.2 13 08-08-01 100 13.4 30.9 2.4 9.6 2.7 14 09-12-01 8.7 13.0 100 28.6 6.6 9.3 15 2.7 9.4 12.8 10-10-01 100 24.9 8.8 9.3 2.5 9.9 12.6 16 11-01-01 100 27.0 6.5 9.1 3.1 11.6 17 12-05-01 100 12.5 24.8 9.1 9.1 4.8 18 19.5 12.9 01-09-02 100 27.5 7.5 9.0 4.5 16.3 13.1 19 02-05-02 100 23.1 9.1 2.6 10.1 11.1 13.0 Note: CV = Coefficient of variation for control reproduction.
On average, the CV for control reproduction is 9.1% in Environmental Testing Solutions, LLC Lower CV bound determined by USEPA
( 10 " percentile) = 8.90.
Upper CV bound determined by USEPA
( 90 t percentile) = 42%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference control and treatment that can be declared between the statistically significant in a whole effluent average, a significant difference occurs for toxicity test. On Environmental Testing Solutions, LLC chronic tests when a toxicant reduces Ceriodaphnia toxicity reproduction by 13.0% from the control.
Lower PMSD bound determined by USEPA (10t percentile) = 11/.
Upper PMSD bound determined by USEPA (90+/- percentile) = 37/.
CT = Central Tendancy (mean Control Reproduction CV or mean PMSD)
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphniareproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent National Pollutant Discharge Elimination Toxicity Applications Under the Program. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH.
02-05-02.xls
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 50 45 40 35 30 25 20 15 10 5
0 Test date
- PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- - Central Tendency (mean PMSD)
........ Lower and Upper PMSD Bounds Lower PMSD Bound (1011 percentile) = 11%, Upper PMSD Bound (909h percentile) = 37%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
Sodfam Chloride Chronic Reference Toxicant Test (EPAJ600'4-91100 2 Method 1002.0)
Speciez. Ceiiodaphniadubla Teat shta Time nalMCdNAC ICR Test NumbJer. 1 Tesa end Diluidon In oration NAQi CHM mnuber- - ----- Conunb.:
Stodc prearation: 100 gN"c~ (&uahs5"S N~al in'-toom!
800tmpe 1000 1200 1400ý St&vbm(L 12 15 18 21 Dilualvolum
- i1491 (;) 142 4.S uR Af
,41994 1-493 Tmf Organism inLormation: Ob- Tat Iniformadon.
Organism aage- q - Rando mizin Date andd timm orgauLms, 02-CfA Te a O:qee33 a2 rsst Incubator nuniber.
were ffbom between: 'ra tto co Organism nbm source:L a Transfer bowl information:
i f'0 YCT batch: A I PH= et Of jempcratmeLC)fz Jp Selenaw-um batch:
r4I%,wTmrr"rIr
..o-z.
Survival and Reproduction Data
CdNaCICR Test Number:. 4_
600 me NaCIIL Survival andReroductlon Data Day 1 2 3 4 5 6 7 1 9 10 I Young produced .0 0 C 0 produced -n eYorg La-AMritmortalfty
,, mortlt
_ _~ L. . L.. L.- L --.- --c... L..
-~Auk Q c ._ , .
6 Yougproduced 17 YIN
ýI oýC C)C 0 0o r-d. M__nee -,*o Aduftortality T1-_ L.T L
%-eutinfooCnrl 7 Young produced ___LA Ilul Adult Mortality f L L.
Adult~ mooratayity:
7 ~ ~ ~ ~ ~ j Ma Offsprodueding/Female:~ j Conedcnrtion:fo onrl Calculations and data reviewed:
800 me NaCI/L Survival andRevroduction Data Day, 1 Replicate number 2 3 4 5 6 7 8 9 10 1 Young produced ~ Q __ _ _
Adk ortaity L L L _
2 Youngproduced m
0 0 c0 0 [o C 0T_
Adult mortality LL . = _- -__-It =,- L =I _ __L =
3 Youn produced 04 0 _
Adult mortality U.. I -- - e -_(_-..
uI. -
E+/- L_.
4 Young produced 2... 2C) l-q 30 [_
Adult mortality T J t T Young produced -7 1 __
Adultmortality g .. . C
= Adoiingproduced ot00 C- = = 'AN C O
"=71~---
Yon Ault mortality Trdcel 10.. It I L V 1 2
=-
t 1 (4 12 Total young protduced 125 M 2 Z. Z5 23 'Iq 7S Iz~Zq Final AdultMortaliyUt ~ 7 ~ 7 1 Concentration:
%Mortality: 07 Mean Offspring/Female: Z2-'7 7%Reduction from Control: 1-18 Calculations and data reviewed: -ý
-I-- - .
CdNaClCR Test Number. I1 1000 m NaCImL I P',I5£15 a2=tI rfu5 Dl.aza D*
- - -- ,-,,., v.vm un*
Yomun produced 4 Dayu mo ta it U _ _ LL FialAdult Mortlt ______
At*Mortity 4 Youngproduced 2-3 .3I ~ % Reduction- 2 from Control: 13.9 I Calculations and data re'viewed: _
1200 mgNaCI/L -~7 7 6 Young produced 0. Survival and Reproduction Data M
Adultmortaity L _36 7 Young 9 10 1 produced Young produced Total youngproduced g'C) 6 ~ ,
p Z0 i.7, 'zo I )4 P FinalAdult S--- Mortality [0) - - - - - 0
,Adult mortlit __L . LU
__L_
- t. UL_
AtMorftafty _ = -F-
_QL.
--Q: L ._.
Adulmortalty L- L \ U 3 Young produced o ( - . * ._ (..
Concentration:
%Mortality: I 0 Mean Oftring/Female: 12.
% Reduction from Control: W.4.
Calculations and data revi ;7.=
0 CdNaCICR Te Number. _.
1400mmNaCT/L Survival andReproductionData T numaber 1 Young produced
- Adlt mo~rtalt 2 oung produced Adn* mortalit 3 Young produced L 4 Young produced Adult mortality 5
- 7. C) nC SL Young produced Adult mor~l 6 Young produced Total young produced Mna Adulth Mo Concentration:
%%Moggaity I Mean Offsp run/eale: A I% Reduction from Control:o7&
Calculations and data reviewed: -
Reproduction ~I~f FY D-c og~_
HI Responset HI UIi 0<
0 c0 8yPa
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubla Quality Control Verification of Data Entry, Calculations, and Statistical Analyses 1'est number: CdNaCICR #19 Dunnett's MSD value: 2.571 MSD = Minimum Significant Difference PMSD: 11.1 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and Ireatmmt that can be decamd statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutioms, LLC cbronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 13.0% from the control.
Lower IIMSD bound determined by USEPA (10b percentile) = 11%.
Upper PMSD bound determined by USEPA (90w percentile) = 37%.
"The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories fir Caelodaphaa trqmodueton in cronic ret~rence toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R.-00R-3. US Favironiaial Protection Agency, Cincinnati, 011.
CdNaCICR Test Number A MO Control Ckemistvy:
[Parameter Control Batch:
o2-p7-+ 2 82-o 1.2..
DO Conductivity (ghscm)
Alkalinity (mg CaCOJL)
Hardness (mg CaCO 3/L) q0 Acceptance Criteriafor Daily Chemistry:
I Acceptance Criteria Concentration (dilutio mus be remade if the conductivity falls outside the acceptable range)
CONTROL 280 - 360 600 mg NaCI/L 1390 - 1590 800 mg NaC1/L 1780-1970 1000 mg NaCI/L 2190 - 2370 1200 mng NaCI/L 2550 - 2740 1400 mg NaCI/L 2910-3160 NaCi Stock 110500 - 116300
CdNaaCcR Test Number _IA CONTROL 600 mg NaCI/L 800 mg NaCI/L
,SrocK cpbsn~fv~r/1 II(4&0 ilcyqoc
CdNaCICP. Test Numbe, 11 17,4co WOW
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
,AddrLes _.B-OX.2Q - - (SUBR 01) OMB No. 2040-0004 If__.INE__RO._EICES__B_..2A_ TN0026450 I1 =G F - FINAL PERMIT NUMBER DISCHARGE NU LOW VOL. WASTE TREATMENT POND
_F_.tjl*_ -J\L SEUQYAUH *J.LER. P.LANT Location HAMILTON COUNTY KArI N IT")fllM(Z PFDhri)r EFFLUENT ATTN: Diedre B. Nida From 02103101 1 T0102103131 YFAF MO [-.YEAR mo I DAY
MEASUREMENT (19) i,."* ..
00530 1 0 0 PiiT 1250 LBS/DY MG/L EFFLUENT GROSS VALUE QUIRMN &A5*S AAI'-4 J'u A 4J'S1 kU*.
-.. ;.f.:.:.:..:.:.:.:.:.:.:.....,.:.:.:.::.:.:..:.:.:.*.*.,.:.:.:... .:;;;............_______::;
UIL ANDU G*.ilASl SAMPLE <52 MEASUREMENT
<49 (26) < 0 <5 (19) (3NAI:3 00556 1 0 0 LBS/DY 307M- MG/L EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU , a;...:*J*:2:,,.;.:****,*....1*:
0 31/31 TOTAL TREATMENT PLANT 50050 1 0 0 (03)
MGD I ******** I EFFLUENT GROSS VALUE SAMPLE MEASUREMENT I I 1M'VII"L.
MEASUREMENT I I I I I SAMPLE MEASUREMENT I I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my 1 direction or supervision in accordance with a system designed to assure that qualified ,6 4 TELEPHONE DATE Richard T. Purcell personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering Site Vice President the information, the information submitted is, to the best of my knowledge and belief, true, Environmental Supervisor 423 843-6700 02 04 11 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshere EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location it Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved.
Name__ TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
- Addess _OX_2_0D (SUBR 01) OMB No. 2040-0004
- - - INTEROFFIC__Bý2). . TN0026450 107 G F F-INAL
_ S - yO _ _TN 373_84
-yi.I PERMIT NUMBER DISCHARGE NUMBER METAL CLEANING WASTE POND Lfocatio JAA -_UY&*LC U _CI--P.-A.NT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT A
ATTN: Diedre 6. Nida From[ 02 03 01 1 Tool02 Y AR 103 131 M(-
NO DISCHARGE E ...
NOTE: Read instructions before coi PARAMETER PH 00400 1 0 0 EFFLUENT GROSS VALUE PHOSPHORUS, TOTAL (AS P)
- I SAMPLE (19) 00665 1 0 0 MEASUREMENT I
- i:MGIL I II EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1 0 0 MEASUREMENT (19)
!i MG/L I I I EFFLUENT GROSS VALUE OIL AND GREASE (19)
I ******** I
- l.ilt 00556 1 0 0 MEASUREMENT I ... -- I 1 (19) 1 1 I EFFLUENT GROSS VALUE COPPER, TOTAL (AS CU) 01042 1 0 0 EFFLUENT GROSS VALUE IRON, TOTAL (AS FE) 01045 1 0 0 EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU 5AMPLE TREATMENT PLANT MEASUREMENT (03) I ...
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50050 1 0 0 ii MGD EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under direction penalty ofinlaw or supervision thatthis document accordance and designed with a system all attachments werethat to assure prepared under my qualified '/ fl i'/'I,7_., TELEPHONE DATE Richard T. Purcell personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, Environmental Supervisor 423 843-6700 02 04 11 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I O U R A NE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshere No phosphate bearing chemicals were employed. No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions mav be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FacilityNamelLocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Nrame TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
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TREATMENT PLANT MEASUREMENT (03) 50050 1 0 0 p*J*ti r : EP:f*T REPORT MGD ****:iI** A.LT EFFLUENT GROSS VALUE A MX !!UIRiMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified
- f. /_ / r TELEPHONE DATE Richard T. Purcell personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering Environmental Supervisor the information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false 02 04 11 SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. S I CERO PF RINCIP A GENT A TYPED OR PRINTED TYEDORPRNTDOFFICER OR AUTHORIZED AGENT AREA{ NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attochments here No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous pditionn.s mav bi us,0 Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FacilityNamelLocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
OMB No. 2040-0004 ALddess -. OX_2_0.. (SUBR 01)
F - FINAL DDY -_SO_.._DL-_D184y__-
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NOTE: Read instructions before completing this form.
PARAMETER DEBRIS, FLOATING (SEVERITY) 31345 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT I I II I II MEASUREMENT S...
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OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.
TYE RPITDOFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshere Operations is required once per shift to perform visual inspections.
EPA Form 3209-1 'REV\ 3/9l1 =4f.^ o,*, 1r,*iH C nWtsJ may) Inuse Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
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NOTE: Read instructions before completing this form.
PARAMETER DEBRIS, FLOATING (SEVERITY) SAMPLE MEASUREMENT
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TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference alt attachments here I ____________________________________________
Operations is required once per shift to perform visual inspections.
EPA Form 3320-1 (REV 3199) Previous editions ma,j be S..................... .
usead Page 1 of 1
PERMITTEE NAME/ADDRESS (include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
hlme_- TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
Add.resýs _BOQX.2, 0 ----
(SUBR 01) 0MB No. 2040-0004
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Location HAMILTON COUNTY EFFLUENT ATfN: Diedre B. Nida From F102ar I103 M0 I1 101 1TO YTARo 02 003131 02 NO DISCHARGE A A R Red'- instrucions lore completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE EX OF TYPE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT (19) 00300 1 0 0 PRI: :: i i. MG/L T:: F EFFLUENT: V E .AI LYGROS 0 SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT ** (19) 00530 .................................
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EFFLUENN wi MOOSTHALLIE FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT (03) 50050 1 0 0 soII REORoEPR MGD r**QN/ BWSM ETM EFFLUENT G RO SS VALUE !))R:Q:: MR: SxMENT M:,AV Al SAMPLE am MEASUREMENT
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER IlCertify under penalty of law thatthis document and all attachments were prepared under m direction
\ ..,4.i/')TELEPHONE DATE Richard T. Purcell personnelorproperly supervision gather in accordance and evaluatewith the ainformation system designed to assure submitted. Based that on my qualified ng inquiry :f tlhe7(4 person or persons who manage the system, or those persons directly responsible for gathering Site Vice President Environmental Supervisor the information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 02 04 11 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICERO TYE O RITDOFFICER R (DRAUTHORIZEDDEAGENIT NAA AREA] NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
EPA Form 3320-1 (REV 3/99) Previouseditions may be used S....
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Page 1 of 1