ML021070745
| ML021070745 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 04/11/2002 |
| From: | Nida D Tennessee Valley Authority |
| To: | Haskin E Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML021070745 (107) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 April 11, 2002 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control 401 Church Street, 6th Floor Annex Nashville, Tennessee 37243-1534 Attention: Ms. Evelyn Haskin, Enforcement & Compliance
Dear Ms. Haskin:
TENNESSEE VALLEY AUTHORITY - DISCHARGE MONITORING REPORT FOR SEQUOYAH NUCLEAR.PLANT Please find enclosed the Discharge Monitoring Report (DMR) of March 2002 for Sequoyah. Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Diedre B. Nida Environmental Supervisor Signatory Authority for Richard T. Purcell Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
>1 Pnnled on recycled paper
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) ae.._
TV__A
- SEQUOYAH NUCLEAR PLANT ALdress_.L X 200
.SQ_ Y.D
. -__AISY - _T-LN i
7314-F. czilit__
A -SEQU_.YAH NU__LEAPt ANT Location HAMILTON COUNTY ATTN: Diedre B. Nida NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
TN0026450 1
1016 PERMIT NUMBER DISCHARGE NUMB F oNmT ORIN G PT RIO D YFAr* I MQ I DAY YEARAI* MO I nAY From 02103101 1 To[02 103 131 MAJOR Form Approved.
(SUBR 01)
OMB No. 2040-0004 F - FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE
-]
NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS FEMPERATURE, WATER DEG.
SAMPLE 15.6 m041 0
31/31 MODELD CENTIGRADE 00010 Z
0 0
INSTREAM MONITORING TEMPERATURE, WATER DEG.
CENTIGRADE 00010 1
0 0
EFFLUENT GROSS VALUE PH 00400 1
0 0
EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1
0 0
EFFLUENT GROSS VALUE OIL AND GREASE 00556 1
0 0
EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU TREATMENT PLANT 50050 1
0 0
EFFLUENT GROSS VALUE CHLORINE, TOTAL RESIDUAL 50060 1
0 0
EFFLUENT GROSS VALUE MESUEEouivINTI I
I I
I SAMPLE MEASUREMENT I
MEASUREMENT 28.5
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" o MEASUREMENT MGIL
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(03)
MGD SAMPLt MEASUREMENT
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MG/L I ---- I ---^ I....
<0.006 0.014 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified Richard T. Purcell personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
TYPED OR PRINTED EPA Iorm su-1 INEV 3I)
Previous edirions may be used Page 1 of 2 4/0 4/31 GRAB 1/0 1
61/61 lmuuur¶L (19)
MGIL COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference a// attachments here No closed mode operation. The following injections occured: 1. PCL-222 (max. calc. conc. was 0.024mg/L--limit 0.100rmg/L) 2. CL-363 (max. calc. conc. was 0.009mg/L--limit 0.100mg/L) 3. PCL-222/PCL-401 (max. calc. conc. was 0.025mg/L--limit 0.100mg/L)
I -********
- et 0
EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 2 I
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different)
TVA - SEQUOYAH NUCLEAR PLANT AA..__des
__.-OX.200
_I NTROC2FFIC. _.-_L) _
__-aO___YD
-AIS)y_
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Faqljrt__/ iA -SEQUOYLArUCLEPLAT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE MONITORING PERIO EFFLUENT Form Approved.
OMB No. 2040-0004 A1rN: Diedre B. Nida YFAR Mo I
YEARI MO I
AY From 02 103 101 1 To 102 103 131 1 NO DISCHARGE
=
NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIM TEMPERATURE - C, RATE OF SAMPLE 0.3 CHANGE 32234 1
0 0
EFFLUENT GROSS VALUE TEMP. DIFF. BETWEEN SAMP. &
LJPSTRM DEG.C
)0016 1
W 0
EFFLUENT GROSS VALUE SAMPLE MEASUREMENT I
- I (0 4 )
1 U 1 I0 / 0 1.f L
DEG. C.
MEASUREMENT MEASUREMENT I
I I
I BORON, TOTAL 01022 1
0 0
EFFLUENT GROSS VALUE
<U.Z I
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(
- 19) 4-1 1/
.1
<0.2 (19) 10 1 /3 MG/L SAMPLE MEASUREMENT I
I I II "NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law thatthis document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified Richard T. Purcell personnel properly gather and evaluate the information submitted. Based on my inquiry of the L) person or persons who manage the system, or those persons directly responsible for gathering Environmental Supervisor Site Vice President the information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 02 04 11 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I
information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED OFCRO UHRZDAET AEj NME ER M
A
-CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here CCW data for March 2002 is attached.
Previous editions may be used EPA porm 3320-1 (REV 3/69)
Page 2 of 2 I
II t*
I I
I I
I I
I MEASUREMENT
CCW Data for March 2002 CCW CHANNEL DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mg/L)
March 13, 2002
< 0.5 CCW TRENCH DISCHARGE DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mq/L)
March 13, 2002 1.0 Page 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Narae TVA - SEQUOYAH NUCLEAR PLANT Ad1dreos
.B.O_.
0
.(I NTE R._QF._FI CC..E.F.3 ]_
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-.N.37384-FaAcIjty_i _VNA -_EUQYAl-L_ L___PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN645 101T F-FINAL PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE
- MONITORING PFRIQQ EFFLUENT Form Approved.
OMB No. 2040-0004 ATfN: Dledre B. Nida Fo YFARm MO AY I oYAR MO I Q~y From 102101101 1 To1 02 03 1311 NO DISCHARGE Liii NOTE: Read instructions before completing this form.
NO.
EX 0
FREQUENCY OF ANALYSIS f
1/90 SAMPLE TYPE COMPOS 0
ýiii i!!"
OM -00 f...
1/90 COMPOS I I I I I
I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law thatthis document and all attachments were prepared under my
(*
I
/
)
TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified Richard T. Purcell personnel properly gather and evaluate the information submitted. Based on my inquiry of the
/
person or persons who manage the system, or those persons directly responsible for gathering Environmental Supervisor Site Vice President the information, the information submitted is, to the best of my knowledge and belief, true, EnvironmentalSupervisor 423 843-6700 02 04 11 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED_______________
OR PRINTED information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER TYPED OR PRINTEDOFIEORATOIEAEN ARA NME YAR M
DY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here Toxicity was sampled from February 24, 2002 through March 1, 2002. Report is attached.
u sIUUO e
SIlIUIa mliay L-UseU I
I EPA Form 3310-1 'REV 3199 I Page I of 1
April 3, 2002 Diedre B. Nida, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, FEBRUARY-MARCH, 2002 Attached are two copies of the subject report for submission to the state of Tennessee. The report provides results of compliance testing using fathead minnows. A copy of the report is included for your records.
Outfall 101 samples collected February 24-March 1, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results indicate that fish pathogen interference is likely the cause of historic problems with WET tests for this effluent.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB 1B-M sqn february 2002m-jm
.1**L TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION/EXECUTIVE
SUMMARY
- 1) Facility/Discharger: Sequoyah Nuclear Plant/TVA Report Date: April 3, 2002
- 2)
County/State: Hamilton / Tennessee
- 3) NPDES Permit #: TN0026450
- 4)
Type of Facility: Nuclear-fueled electric generating plant
- 5)
Design Flow (MGD): 3,266
- 6)
Receiving Stream: Tennessee River (TRM 483.6)
- 7)
IQ10:2,992.4
- 8)
Outfall Tested: 101
- 9) Dates Sampled: February 24-March 1, 2002
- 10) Flow on day(s) sampled (MGD): 1624, 1614, 1569
- 11) Pertinent site conditions: No unusual conditions reported. *
- 12) Test Dates: February 26-March 5, 2002
- 13) Test Type: Short-term Chronic-definitive
- 14) Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia)
- 15) Concentrations Tested (%): Outfall 101: 10.98, 22, 43.9, 72, 100 Intake: 100 Pimephalespromelas: UV treated Outfall 101: 10.98, 22, 43.9, 72, 100 UV treated Intake: 100
- 16) Permit Limit Endpoint (%): Outfall 101 IC,>5 = 43.9
- 17) Test Results (%): Outfall 101: Pimephalespromelas: IC2_Q 5 > 100 Ceriodaphnia dubia: IC25 > 100 UV treated Outfall 101: Pimephales promelas: ICQ 25> 100
- 18) Facility
Contact:
Diedre B. Nida
- 19) Phone #: (423) 843-6700
- 20) Consultant/Testing Lab Name: Environmental Testing Solutions, LLC
- 21) Lab
Contact:
Jim Sumner
- 22) Phone #: (828) 862-8193 TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- Production/operation data will be provided upon request.
Notes: Outfall 101 samples collected February 24-March 1, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms. These results indicate that fish pathogen interference is likely the cause of historic problems with WET tests for this effluent.
METHODS
SUMMARY
Samples
- 1) Sampling Point: Outfall 101
- 2) Sample Type: Composite
- 3) Sample Information:
Sample Date/Time Date/Time Arrival Initial Date/Time ID Collected Received Temp.
TRC*
Used (MM-DD-YY/Time)
(MM-DD-YY/Time)
(0C)
(mg/L)
(MM-DD-YY/Time) 11 02-24-02/1454 & 1518 02-26-02/1 105 0.9
<0.10 02-26-02/1522 02-25-02/0354 & 1418 02-27-02/1514 Intake 02-24-02/1539 02-26-02/1105 0.6
<0.10 02-26-02/1522 02-25-02/1439 02-27-02/1514 101 02-26-02/1026 02-28-02/1045 0.2
<0.10 02-28-02/1516 02-27-02/0926 03-01-02/1500 Intake 02-26-02/1038 02-28-02/1045 0.7
<0.!10 02-28-02/1516 02-27-02/0938 03-01-02/1500 101 02-28-02/1335 03-02-02/1058 0.6
<0.10 03-02-02/1506 03-01-02/1235 03-03-02/1453 1
03-04-02/1456 Intake 02-28-02/1405 03-02-02/1058 1.1
<0. 10 03-02-02/1506 03-01-02/1305 03-03-02/1453 03-04-02/1456
- Total residual chlorine.
"Collected in two 2.5-gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4) Sample manipulation:
Routine samples from Outfall 101 and intake were warmed to test temperature (25.0 +/-1.00C) in a warm water bath.
Aliquots of Outfall 101 and intake samples used for the side by side UV treated test were warmed to 24°C in a warm water bath and held in a 40Watt Rainbow Lifeguard UV Sterilizer for 2 minutes, prior to exposure of test organisms.
2
Test Organisms
- 1) Source:
- 2) Age:
Pimephales promelas Aquatic BioSystems, Inc.
23 hours2.662037e-4 days <br />0.00639 hours <br />3.80291e-5 weeks <br />8.7515e-6 months <br /> Ceriodaphnia dubia In-house culture
< 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> Test Method Summary (See Appendix A for additional information)
- 1) Test Conditions
- 2) Test Duration
- 3) Dilution/Control Water Pimephales promelas Static, renewal Ceriodaphnia dubia Static, renewal 7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic Water Moderately Hard Synthetic Water
- 4) Number Replicates
- 5) Animals per Replicate
- 6) Test Initiation: (Date/Time)
Outfall 101 02/26/02-1438 EST UV Treated Outfall 101 02/26/02-1438 EST
- 7) Test Termination: (Date/Time)
Outfall 101 03/05/02-1430 EST UV Treated Outfall 101 03/05/02-1430 EST
- 8) Test Temperature: Outfall 101 Mean =24.6 'C (24.1-25.2°C) 02/26/02-1522 EST 03/05/02-1520 EST Mean = 24.6°C (24.2-25.3-C)
Test Temperature: UV-Treated Outfall 101 Mean = 24.70 C (24.2-25.5 0C)
- 9) Physical/Chemical Measurements: Hardness, alkalinity, total residual chlorine, and conductivity were measured at the laboratory in each 100 percent sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10) Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
3 4
10 10 1
TOXICITY TEST RESULTS (See Appendix C for Bench Sheets)
- 1) Results of a Pimephales _promelas (Genus)
(Species)
Conducted 02/26/02 03/05/02 (mm/dd/yy) (mm/dd/yy)
Chronic7-d Tox (Type/Duration)
Using Effluent From Outfall icity Test 101 (number)
Percent Surviving Test Solutions (time intervals used - days) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98% Effluent 100 100 100 100 95 92 87 22% Effluent 100 100 100 100 100 100 100 43.9% Effluent 100 100 100 100 100 100 100 72% Effluent 100 100 100 100 100 100 98 100% Effluent 100 100 100 100 95 95 93 Intake 100 100 100 100 100 100 100 IC 25 Mean Dry Weight (mg)
Test Solutions (Replication) 1 2
3 4
j Mean Control 0.702 0.646 0.618 0.494 0.615 10.98% Effluent 0.555 0.597 0.611 0.330 0.523 22% Effluent 0.580 0.551 0.522 0.535 0.547 43.9% Effluent 0.571 0.530 0.626 0.543 0.568 72% Effluent 0.579 0.619 0.693 0.560 0.613 100% Effluent 0.685 0.626 0.652 0.387 0.587 Intake 0.537 0.682 0.525 0.591 0.583 IC25 Value: > 100%
Calculated TU Estimates: t < 1.OTUc 95% Confidence Limits:
Permit Limit: 1.OTUc Upper Limit = N/A Lower Limit = N/A tTUc = 100/IC25 4
TOXICITY TEST RESULTS (continued)
- 2) Results of a Ceriodaphnia (Genus) dubia Chronic 3-brood (Species)
(Type/Duration)
Toxicity Test Conducted 02/26/02 03/05/02 (mm/dd/yy) (mm/dd/yy)
Using Effluent From Outfall Percent Surviving Test Solutions (time intervals used - days) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 6.25% Effluent 100 100 100 100 100 100 100 12.5% Effluent 100 100 100 100 100 100 100 25% Effluent 100 100 100 100 100 100 100 50% Effluent 100 100 100 100 100 100 100 100% Effluent 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) Data Test Solutions Replicates 1
2 3
4 5
6 7
8 9
10 Mean Control 22 25 26 23 24 22 26 21 20 25 23.4 10.98% Effluent 21 22 23 23 23 24 22 23 28 24 23.3 22% Effluent 26 24 24 23 24 21 25 25 23 27 24.2 43.9% Effluent 28 23 26 26 29 22 24 22 28 27 25.5 72% Effluent 26 30 26 30 26 28 27 30 31 28 28.2 100% Effluent 31 32 27 29 28 31 30 29 27 28 29.2 Intake 26 27 28 27 30 29 31 23 31 30 28.2 IC25 Value: > 100%
Calculated TU Estimates: t < 1.0TUc c 95% Confidence Limits:
Permit Limit: I.0TUc Upper Limit = N/A Lower Limit = N/A t TUc = 100/IC 25 REFERENCE TOXICANT TEST RESULTS (See Appendixes A and D)
Species Date Time I Duration I Toxicant Results (IC25)
Pimephales promelas 02/26/02 143 8 7-days KCI 561.38 mg/L (IC
- 25)
Ceriodaphnia dubia 02/05/02 1337 7-days NaCI 1072.5 mg/L (IC 25) 5 101.
TOXICITY TEST RESULTS UV-TREATED (See Appendix C for Bench Sheets) 1I)
Results of a Pimephales promelas Chronic7-d Toxicity Test (Genus)
(Species)
(Type/Duration)
Conducted 02/26/02 03/05/02 Using Effluent From Outfall 101, UV treated.
(mm/dd/yy) (mm/dd/yy)
(number)
Percent Surviving Test Solutions (time intervals used - days) 2 3
4 5
6 7
Control 100 100 100 100 100 100 100 10.98% Effluent 100 100 100 100 100 100 100 22% Effluent 100 100 100 100 98 98 98 43.9% Effluent 100 100 100 100 100 100 100 72% Effluent 100 100 100 100 100 100 1o0 100% Effluent 100 100 100 100 o00 100 100 Intake 100 100 100 100 100 100 100 IC2 5 Mean Dry Weight (mg)
Test Solutions (Replication) 1 2
3 4
Mean Control 0.525 0.476 0.472 0.433 0.476 10.98% Effluent 0.493 0.520 0.559 0.584 0.539 22% Effluent 0.487 0.455 0.486 0.621 0.512 43.9% Effluent 0.716 0.493 0.510 0.542 0.565 72% Effluent 0.611 0.579 0.465 0.503 0.540 100% Effluent 0.501 0.511 0.516 0.595 0.531 Intake 0.558 0.587 0.541 0.522 0.552 IC2 5 Value: > 100%
Calculated TU Estimates:
< l.OTUc 95% Confidence Limits:
Permit Limit: 1.OTUc Upper Limit = N/A Lower Limit = N/A TUc = 100/IC25 6
SUMMARY
/CONCLUSIONS Outfall 101 samples collected February 24-March 1, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results indicate that fish pathogen interference is likely the cause of historic problems with WET tests for this effluent.
7
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1) Pimephales promelas Tests were conducted according to EPA/600/4-91/002 (July 1994) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.
- 2) Ceriodaphnia dubia Tests were conducted according to EPA/600/4-91/002 (July 1994) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 3 0-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS/MODIFICATIONS TO TEST PROTOCOL
- 1) Pimephales promelas None
- 2) Ceriodaphnia dubia None DEVIATIONS/MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1) Pimephales promelas None
- 2) Ceriodaphnia dubia None 8
PHYSICAL AND CHEMICAL METHODS
- 1) Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2) Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3) Temperature was measured using EPA Method 170.1.
- 5) The pH was measured EPA Method 150.1.
- 6) Conductance was measured EPA 120.1.
- 7) Alkalinity was measured using EPA 310.1.
- 8) Hardness was measured EPA Method 130.2.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA/600/4-91/002. Any known deviations were noted during the study and are reported herein.
9
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1) Test Type: 7-day chronic tests with results expressed as IC25 values in g KCI.
- 3) Dilution Water Used: Moderately hard synthetic water.
- 4) Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
REFERENCES
- 1) NPDES Permit No. TN0026450.
- 2) Lewis, P. A., D. J. Klemm, J. M. Lazorchak, T. J. Norberg-King, W. H. Peltier, M. A. Heber.
Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA/600/4-91/002 (July 1994).
- 3) Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
10
Sequoyah Nuclear Plant Biomonitoring February 26-March 5, 2002 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, And Initial and Final Chemistry for Fathead Minnow 7-day and Daphnid 3-brood Chronic Tests
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-March 1, 2002 Dat ;~Sohui<~. ATowerbrom ~PFL-222 mg/L TRC ngL Ph2s hate 03/12/1998 0.016 03/13/1998 0,015 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.013 8 03/17/1998 0.020 03/18/1998 0.018 09/08/1998 0,015 0,014 09/09/1998 0.003' 0.031 09/10/1998 0.014 0.060 09/11/1998 0.013 0.055 09/12/1998
< 0.001 0.044 09/13/1998
< 0.001 0.044 09/14/1998 0.008 0.044 02/22/1999
<0.001 02/23/1999 0.005 02/24/1999 0.009 02/25/1999 0.012 02/26/1999 0.008 02/27/1999
< 0.001 02/28/1999
<0.001 08/18/1999 0.015 0.069 08/19/1999 0.012 0.068 08/20/1999 0.023 0.070 08/21/1999 0.022 0.068 08/22/1999 0.022 0.068 08/23/1999 0.025 0.068 08/24/1999 0.016 0.067 PCL-401 mg/L Opolymer 0.005 0.011 0.021 0.019 0.015 0.015 0.015 0.024 0.024 0.024 0.024 0.024 0.024 0.023 CL-363 DMAD-0.006 0.020 Cuprostat- ýý, *0M PF mg/L Azole rh 0ua1
ý-0.021 0.120 H
i:
i
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-March 1, 2002 nypqchmonte m~EC Towerbrom PýCL;-222 mg/L TRC PCL-401
- CL-363 mg/LgL:
i Co..polymrer j3A uivu fQuat~
01/31/2000
< 0.002 0.009 02/01/2000 0.011 0O.026 0.028 02/02/2000 0.028 0.026 0.009 0.006 02/03/2000 0.008 0.027 0.009 02/04/2000 0.006 0.027 0.009 0,005 0.109 02/05/2000
< 0.002 0.027 0.009 02/06/2000
< 0.002 0.027 0.009 07/26/2000
<0.0057 0.055 0.019 0.021 07/27/2000 0.019 0.055 0.019 07/28/2000 0.0088 0.053 0.018 0.004 0.108 07/29/2000
< 0.0088 0.055 0.019 07/30/2000
< 0.0076 0.055 0.019 07/31/2000
< 0.0152 0.055 0.019 0.006 08/01/2000
< 0.0141 0.055 0.019 0.005 12/11/2000 0.0143 0.025 0.020 0.005 o
12/12/2000 0.0092 0.025 0.020 0.005 12/13/2000
< 0.0120 U25 0.020 12/14/2000
<0.0087 0.025 0.020 12/15/2000 0.0120 0.025 0.020 0.005 12/16/2000 0.0036 20.020 12/17/2000
<0.0036 0.025 0.020
<0.036 0.025:
0.020 08/26/2001 0.017 0.06 0.021 0.006 08/27/2001
<0.0096 0.06 0.021 0.005 0.021 08/28/2001
<0.0085 0.06 0.021 08/29/2001
<0.0094 0.059 0.020 0.005 0.021 08/30/2001
<0.0123 0.06 0.021 0.005.
08/3 1/2001
<0.005 0.059 0.020 11/25/2001
-<0.0044 11/26/2001
<0.0119 0.024 0.02 005 11/27/2001 0.0137 0.023 0.019 0.007 11/28/2001
<0.0089 0.022 0.019 0.006 11/29/2001 0.0132 0.024 0.02 0.007 11/30/2001
< 0.0043 0.024 0.02 12/09/2001[
T -nnA' n 1
<0.0042
<0.0 104 0.0128
<0.0088 0.0134 0.024 0.024 0.024 0.02 0.02 0.02 0.008 0.007 Date Cuprostat PF mg/L Azole H-I OM 12/10/2001 12/11/2001 12/12/2001 12/13/2001 12/14/2001
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-March 1, 2002 Date
':"Sodiuiium
- Towerbrom "CL-22> PCL-40.1
'C-,3.63.
Guprostat-
-.13. ý,
hypochlorte~ mg/
R mg/L-fýL PF mg/L m
ni,5*
C Psa Copolymer DMAD 1/02/2002
< 0.0079
> -0.023 0.02 06 1/03/2002
<0.0042 0.023 0.014 1/04/2002 0.0124 0.024 0.014 0.009 1/05/2002
<0.0042 1/06/2002
<0.0042
-0 1/07/2002
< 0.0089 0.024 0.014 0.006 2/24/2002
< 0.004 2/25/2002
< 0.004 0.023 0.023 2/26/2002 0.0143 0.023 0.023 0.007.
2/27/2002
<0.0041 0.023 0.023 2/28/2002 0.0041 0.024.
0.008 3/01/2002
<0.0041 0.024 0.008
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pinmephales promelas and Ceriodaphnia dubia Tests, Sequoyah Nuclear Plant Effluent (SQN),
Outfall 101, February 26-March 05, 2002 Total Temperature Dissolved Oxygen pHu Conductance Alkalinity Hardness Residual Test/
Initial Final Initial Final Initial Final Initial Chlorine Sample ID (OC)
(OC)
(mg/L)
(rag/L)
S.U.
S.U.
([tmhos) mg/L CaCO 3 mg/L CaCO3 (mg/L)
Fathead/
24.6 24.4 8.0 7.4 8.10 7.86 315 67 86 Control (24.3-25.0)
(24.2-24.7)
(7.6-8.2)
(7.0-7.8)
(8.08-8.11)
(7.65-8.02)
(309-323)
(65-69)
(86-86)
Fathead/
24.7 24.4 8.0 7.4 8.10 7.8.1 308 10.98 %
(24.4-25.0)
(24.2-24.7)
(7.8-8.1)
(6.5-7.9)
(8.08-8.12)
(7.52-8.00)
(302-322)
Fathead/
24.7 24.4 8.0 7.4 8.09 7.80 295 22 %
(24.4-24.9)
(24.2-24.7)
(7.9-8.2)
(6.4-7.9)
(8.06-8.12)
(7.50-8.00)
(289-307)
Fathead/
24.8 24.4 8.1 7.4 8.04 7.78 264 43.9 %
(24.5-25.0)
(24.2-24.7)
(8.0-8.3)
(6.5-7.9)
(7.98-8.08)
(7.49-7.95)
(260-272)
Fathead/
24.8 24.4 8.2 7.4 7.98 7.76 223 72%
(24.4-25.1)
(24.1-24.7)
(8.0-8.5)
(6.4-7.9)
(7.88-8.03)
(7.46-7.95)
(220-228)
Fathead/
24.9 24.4 8.3 7.4 7.90 7.74 180 55 69
<0.10 100%
(24.5-25.2)
(24.2-24.7)
(8.1-8.6)
(6.9-7.7)
(7.75-7.99)
(7.48-7.94)
(177-183)
(54-56)
(66-70)
(<0.10-<0.10)
Fathead/
24.8 24.4 8.3 7.5 7.88 7.77 177 56 70
<0.10 Intake (24.3-25.2)
(24.2-24.7)
(8.1-8.6)
(7.2-7.8)
(7.73-7.99)
(7.47-8.01)
(175-182)
(54-59)
(66-76)
(<0.10-<0.10)
Daphnid/
24.5 24.4 8.0 8.1 8.10 8.12 315 67 86 Control (24.3-24.7)
(24.2-24.6)
(7.6-8.2)
(8.0-8.2)
(8.08-8.1 1) (8.07-8.17)
(309-323)
(65-69)
(86-86)
Daphnid/
24.6 24.4 8.0 8.1 8.10 8.13 308 10.98 %
(24.5-24.9)
(24.2-24.6)
(7.8-8.1)
(8.0-8.2)
(8.08-8.12)
(8.07-8.18)
(302-322)
Daphnid/
24.6 24.4 8.0 8.1 8.09 8.13 295 22 %
(24.4-24.9)
(24.2-24.6)
(7.9-8.2)
(8.0-8.2)
(8.06-8.12)
(8.07-8.14)
(289-307)
Daphnid/
24.7 24.4 8.1 8.2 8.04 8.13 264 43.9 (24.4-25.0)
(24.2-24.6)
(8.0-8.3)
(8.1-8.2)
(7.98-8.08)
(8.06-8.20)
(260-272)
Daphnid/
24.8 24.4 8.2 8.2 7.98 8.08 223 72 (24.5-25.0)
(24.2-24.6)
(8.0-8.5)
(8.0-8.3)
(7.88-8.03)
(7.99-8.14)
(220-228)
Daphnid/
24.8 24.4 8.3 8.2 7.90 8.02 180 55 69
<0.10 100%
(24.6-25.0)
(24.2-24.6)
(8.1-8.6)
(8.0-8.4)
(7.75-7.99)
(7.90-8.10)
(177-183)
(54-56)
(66-70)
(<0.10-<0.10 Daphnid/
24.8 24.4 8.3 8.2 7.88 8.11 177 56 70
<0.10 Intake (24.4-25.3)
(24.2-24.6)
(8.1-8.6)
(8.0-8.3)
(7.73-7.99)
(7.99-8.22)
(175-182)
(54-59)
(66-76)
<0 1.0-<0. 10)
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephalespromelas Test, Sequoyah Nuclear Plant (SQN),
UV Treated Outfall 101 and Intake Samples, February 26-March 5, 2002 Total Temperature Dissolved Oxygen pH Conductance Alkalinity Hardness Residual Test/
Initial Final Initial Final Initial Final Initial Chlorine Sample ID (oC)
(OC)
(mg/L)
(mig/L)
S.U.
S.U.
(jtmhos) mg/L CaCO3 mg/L CaCO3 (mg/L)
Fathead/
24.6 24.4 7.9 7.4 8.01 7.86 319 67 86 Control (24.3-24.9)
(24.2-24.7)
(7.6-8.1)
(6.8-8.0)
(7.93-8.11)
(7.60-7.99)
(308-340)
(65-69)
(86-86)
Fathead/
24.8 24.4 7.9 7.4 8.04 7.85 310 10.98%
(24.4-25.0)
(24.2-24.7)
(7.6-8.1)
(6.7-7.9)
(7.95-8.11)
(7.59-7.98)
(304-324)
Fathead/
24.9 24.4 7.9 7.4 8.05 7.82 295 22 %
(24.3-25.2)
(24.2-24.6)
(7.6-8.2)
(6.6-7.9)
(7.96-8.11)
(7.58-7.96)
(289-306)
Fathead/
24.9 24.4 8.0 7.5 8.03 7.81 264 43.9%
(24.5-25.2)
(24.2-24.6)
(7.6-8.2)
(6.6-7.9)
(7.95-8.08)
(7.58-7.94)
(260-271)
Fathead/
25.0 24.6 8.0 7.4 7.99 7.77 222 72%
(24.4-25.4)
(24.2-25.5)
(7.6-8.4)
(6.7-7.9)
(7.91-8.04)
(7.55-7.91)
(219-225)
Fathead/
25.1 24.4 8.1 7.5 7.93 7.76 180 55 69
<0.10 100%
(24.8-25.4)
(24.2-24.6)
(7.6-8.4)
(7.1-7.8)
(7.85-7.99)
(7.54-7.88)
(177-185)
(54-56)
(66-70)
(<0.10-<0.10)
Fathead/
25.0 24.4 8.1 7.6 7.92 7.75 177 56 70
<0.10 Intake (24.7-25.3)
(24.2-24.6)
(7.8-8.5)
(7.2-7.9)
(7.82-7.98)
(7.52-7.90)
(174-182)
(54-59)
(66-76)
(<0.1 0-<0. 10)
Sequoyah Nuclear Plant Biomonitoring February 26 - March 05, 2002 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD Page I
of 1
Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):
Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN C o n ta in er Field Identification o.Grab/ Ship.
Collection Date/Time Number &
Flow Rain Event?
Sample Description Comp.
Temp. (Volume (MGD)
(Mark as Appropriate)
- ~...
0C)
Collected Si~i it End ii ii y.i En Y
If Yes, No TEr itT.a rw
~
m
~~ ~ ~
Inch;;.
SQN-101-TOX comp 4
02
/,p 02
~
(2.5g)
N."~
SQNNT-TOX comp 4
42
/
1 (2.5g)
Sample Custody - Fill In From Top Down Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time t-ed e*
oz-z-,o 116s-
/,ffi otW Je.-4S Oz,-z, Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 40C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with amnple time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1
of I
Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):
Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN C ontain r
...*..*:*`..*::*.*
Container X
Field Identification /
Grab/
Ship.
ColcinDt/ie Number &
Flow Rain Event?
-X...
Sample Description Comp.
Temp. (
Volume (MGD)
(Mark as Appropriate)
°C_
Collected Str if Yes, No Trace ST.g Av1np(1y ue Apaz
/q.....
.2/27/Qk SQN-101-TOX comp 4
2/26/02 Oft&
'!,,(2.5g) io'4 i
SQN-INT-TOX comp 4
2-/
1 (2.5g)
~
Sample Custody - Fill In From Top Down Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time el,2-28
()O5 o____________
O29-02 lO0tS Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 40C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page I
of 1
Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):
Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN Container Field Identification /
Grab/
Ship.
Collection Date/Time Number &
Flow Rain Event?
Sample Description Comp.
Temp. (Volume (MGD)
(Mark as Appropriate)
.tt 0_____)_
Collected____
.trtI Y.,
N TramcAwTmp pe v...-...-......-..-...
Inch es
/5
- 3/1/02 SQN-101-TOX comp 4
2m-z5 2 (2.5g)
~Q~
- ~
SQN-INT-TOX comp 4
/2970 3/(/02g)
Sample Custody - Fill In -From Top Down Relinquished By (Signature):
Date/Time Received By (Signature):
DateTime Saml D C
T(Vu)a p
t_
67....
O::
- ::::::: ::: :: :: ::: :: :: ::: ::::::: :: :: ::::: :::::::::::::::::D::::
instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 4"C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Sample Receipt Log Date Time Received Received Sample Project Sample onn Received Received By From Temperature Nme Nubr SplNaendDsitonState Cmet Numbe Number SampleNameanDecito.o aO'i4, AL*',-,
'-.QCAh1Me 2.1*C.
Z 0,
oZof02 1, E7,AXW EtjV. -
IMAIS4AU. LLf lwro C
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02.14-0o 113 KEKe~jUAf31 ebe-X I*O'C.
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O2I~-o2 112-0 1
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a Environmental Testing Solutions, LLC Received By a*L*QJM
-KE iNAO KEKoet'M Received From Febe~x
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Febe/
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Sample Receipt Log Project Sample SapeNmanDecito Number Number SapeNmanDscito
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~TVA SekJcjIAW e
TVAJ<P-f4 I Al '4 cjrft^A Is1.APIT 20~ 020SO2.O2.
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210 0 O3OS.O1 ALJIuA Co.be. S0LA.
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Received LIV2M -2
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I Dilution preparation information:
Comments:
Mh batch:
O T-I-oY* -
U2J-.,-I_7-,
Dilutionpccp(%)
eI.q*I 2Z 17_
t EfenvW(mL) IS1.1
-36 145z.2___________
Diluet Volume (mIQ
,4 q2E 1I.
Test organism information: Cv 64t Test information:
Organism age:
2321f4piS Ot Randomizing template:
G Date and times organisms 5Z-2 1-Ot 1530 7;6 4¢*
Incubator number:
z were born between:
I "S3 oo oO r-S r
Organism source:
ASS L*.tC
- ?-is:6Z.
Artemia lot number:
iP du-??
Transfer bowl information:
pH =
Temperature =
Oven temperature:
- 60.
7M21.V C-Dryig time:
Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time i
GZ-Z."-0o.
0*i 1qO 1"
I 1
2 a
cjaq-t-29Q W41 q5D
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_0__7day_
C50____'
AvrA 0w r.0O 6'
654;c.
PA 1(
IControl infoirmation:
Acetac criteria Summar of test endpoints Average weight pr larvae:
- 0.
5>0.25 moarvae NOEC 0*f ChV
> /0o IC25
> /60%
LOEr Chronic Whole Effluent Toxicity Test (EPA/600/4-911002 Method 1000.0)
Species: Pimephales promelas Client: -WT,
AA AXW ?*AM-.
Facility:
No/e
'RiaATeb NPDES #: -M
- 002OW4SQ, L %%o j. v.L u
-7/00 -,Z,
Environmental Testing Solutions. LILC Envronentl tng S
s, -rage 2 of 8 Species: Pimephalespromelas Client: "T-VA-Seja-ANtA Date:
OZ-zg0.7 Survival and Growth Data Day CONTROL
__.4o 2 z A
B C
D E
F G
- H J
K L
0 tO
- l0 0 10
/0(010
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'ow/
1
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-0 0 /0 to 10 to
/0 10 /0 0
/0 2
0o 0!
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/
00
/0 16 10
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/0
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/0 10 10 10 to qI0 IL)10 (5i IN 10 I1 10
!0 10 lo Io 10 10 10 "14 IQ
!0
/0 6
7b 10 1i 10 1
t 10 lb 1010 10 tO/
B = Pan + Larvae weight (mg) 6 A(
Larvaeweigjhit(mg)=A-B
.E&L j06 20.
7.'4 Calculations and data reviewed:
Comments:
Vi.
I S........
Environmental Testing Solutions. LLC rage 3 Of 8 Species: Pimephales promelas Client: "r"VA- _nUa 4AA Date:
0?-- ZIP-Oz Survival and Growth Data Day
,77-76 106 M
N 0
P Q*
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0 1 0 /0
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0 1c/
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+e wd Lavewigt(g Comments:
-)t da.
Species: Pimephales promelas Client: -TVP S_*nuiNAA Date:
OZZ,*,O*
Calculations and data reviewed: 4 Comments:
0 Q I I I I
,,nvhvnmeIILaU eSLIUr.. 10 UL U113, bnvtronmemm x un mH,.auu,....,.
- 1, Ims
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pinephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client:
TVA Test dates:
Febr Project number:
269 Outfall loll Dunnett's MSD value:
PMSD:
Jntake.
Dunnctl MSD value:
PMSD:
Sequoyah Nuclear Plant, Nontreated uary 26-March 5, 2002 MSD =
PMSD:
0.1544 25.1 0.1102 17.9 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and beatracnt that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occus for Environmeaal Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.5% from the control (determined Lower PMSD bound determined by USEPA (10th percentile) = 9.4%
Upper PMSD bound determined by USEPA (90th percentile) = 35%
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laborato:ies for'Pimqhalea growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Proteclion Agency, Cincinnati, OIf.
Concentratton (%)
Replicale tttialtnumberof Flaal nwberoftarvae A - Pau ightt B - Pan Larae Larvae wesht n()
Weight /liiala number Mea urvtval Mas weltkl (n)
CndmtO Percet rndedain A*=
larvae (ag)
.eighl (.&)
-A-B oftava¢(nmg)
(%)
nrall
(%)
mI
(%)
A 10 10 15.038 22.060 7.022 0.7022 Control B
10 10 14.940 21.400 6.460 0.6460 100.0 0.6151 14.3 Not applale C
10 10 14.970 21.150 6.180 0.6180 D
10 10 15.070 20.010 4.940 0.4940 E
10 9
14.708 20.260 5.552 0.5552 10.98%
F 10 10 14.848 20.820 5.972 0.5972 86.4 0.5232 25.0 14.9 G
10 10 14.715 20.820 6.105 0.6105 H
9 5
14.681 17.650 2.969 0.3299 1
10 10 15.085 20.880 5.795 0.5795 22%
J 10 10 14.646 20.160 5.514 0.5514 100.0 0.5471 4.5 11.0 K
10 10 14.657 19.880 5.223 0.5223 L
10 10 14.627 19.980 5.353 0.5353 M
10 10 14.727 20.440 5.713 0.5713 43.9%
N 10 10 14.621 19.920 5.299 0.5299 100.0 0.5675 7.5 7.7 0
10 10 14.847 21.110 6.263 0.6263 P
10 10 14.834 20.260 5.426 0.5426 Q
10 9
14.936 20.730 5.794 0.5794 72%
R 10 10 14.814 21.000 6.186 0.6186 97.5 0.6128 9.6 OA S
10 10 14.800 21.730 6.930 0.6930 T
10 10 15.039 20.640 5.601 0.5601 1
U 10 10 14.851 21.700 6.849 0.6849 100%
V 10 10 14.981 21.240 6.259 0.6259 92.5 0.5874 23.1 4.5 W
10 10 15.051 21.570 6.519 0.6519 X
10 7
14.642 18.510 3.868 0.3868 Y
10 10 15.095 20.460 5.365 0.5365 100% Intake Z
10 10 14.983 21.800 6.817 0.6817 100.0 0.5834 12.3 5.1 AA 10 10 14.945 20.190 5.245 0.5245 BB 10 10 14.910 20.820 5.910 0.5910 1
1 Revelwed br. /' I
Environmental Testing Solutions, LLC Statistical Analyses lanrva F=J Growth ad Sa-wvval Ta-7 Day Surviva Str Dar.
2/2a02 Teat M:
PpFRCR Samn* 1D" IVA SQN, Oufal 101 - NouIa-emd End Datt 3/St02 Lab Mt MTS-Env. Testing Solutions Sample TyDpe:M,-Discharge Monitoring Report Sample DaW.
Protoco:
EPAP 9 -EPA Freahwatm Tea Specim:
PP-Pimephales premects Commenm:
Come-%
1 2
3 4
D.Conutol 1.0000 1.0000 3.00)0 1.0000 10.98 0.9000 1.0000 1.0000 0.5556 22 1.0000 1.0000 1.0000 1.0000 43.9 1.0000 1.0000 1.0000 1.0000 72 0.9000 1.0000 1.0000 1.0000 100 1.0000 1.0000 1.0000 0.7000 Transform: Arculn Square Root Rank 1-Talled Conc-%
Mean N-Mean Mean Mi Max CV%
N Sam Critical D-Control
.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
10.98 0.8639 0.8639 1.2285 0.8411 1.4120 21.936 4
14.00 10.00 22 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
18.00 30.00 43.9 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
18.00 10.00 72 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4
16.00 10.00 100 0.9250 0.9250 1.3068 0.9912 1.4120 16.103 4
16.00 10.00 Auxiliary Tests statistic Critical Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p < 0.01) 0.75127089 0.884
-1.747871287 4.247234099 Equality of variance cannot be confurmed Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU Steel's Many-One Rank Test to0
>100 I
Dose-Response Plot 0.9 0.8 0.7
>0.6 S0.5 I*0.4.
0.3 0.2 0.1 0
r*N sqnO2-26-02.xls
Environmental Testing Solutions, LLC Statistical Analyses LSaVYl Fh Grnwlh lad Sunfrui Tes8-7 Day Grmh SWDm 22&W2 Tom ID.
8mh 1D TVA SW. Ow 101. N-atod EWdDUE 3/=~0 Lab IDk ITS-W. Tuft So1WIOII SAM*pi Two DMR.Dkdmar M-Wzingo R" S=Wk Dow PtImOOL.
EPAT91-EPAPRubhw Ted Specdt:
PP-Ptdwm h
prowd" Cmo.%
1 2
4 13CO1 0.7022 0.6460 0.6180 0.4940 10.9 0.s52 0.5972 0.6105 03299 22 0L75 0.5514 O023 0.5"353 43.9 O.513 03.5299 O.6263 05426 72 0.79 0.6186 0.6930 0,5601 100 0.6849 0.6259 0.519 03868 TranfoM UnraISMlmed Rank 1-Talled 11o0o41C Cono.%
Me*
NMM Mean 4
Max CV%
N Sum Critkcl Man N-M..
D..Czarol 0.6151 1.0000 0.6151 0.4940 0.7022 14.302 4
0.6151 1.0000 10.98 0.5232 0.8507 0.5232 03299 0.6105 25.040 4
13.00 10.00 0.5676 0.9229 22 0.5471 0.8896 0.5471 0.5223 0.57O 5 4.505 4
14.00 10.00 0.5676 0.9229 43.9 0.5675 0.9227 0.675 0.5299 0.6263 7.548 4
15.00 10.00 0.5676 0.9229 72 0.6128 0.9963 0.6128 0..601 0.6930 9.589 4
17.00 10.00 0.5676 0.9229 1OD 0.5874 0.9550 0.5874 03868 0.6849 23.133 4
10.00 10.0) 0.5676 0.9229 Auxiliary Tet Statiutic Critical Skew Kur Shapi,-Wdks Tet imdicaa nonnal di'nibo' (p ' -0,01) 0.87742573 0.384 246332l7 1.425134333 Bauldens Toa u equal vjnraow (p - 0.10) 9.240524292 15.08631706 Hypodwis Tat (1-44 0.09)
NOEC LOEC ChV TU Stad's Mwy-Oo Rank Test 100
'100 1
im-a Instpolaon (9) RIsnpift)
Point SD 9"% C*(Exp)
Skew 1C05" 7.1161 ICIO
>100
[Cis
'100 1.0 1C20
>t00 1C25
>100 0.9 IC40
>100 0.8 IC50
>100 0.7 ndCates IC csbtazc l=ss han ote lowen conom~on0.
a0.6.
0.5 0.3 0.2 0.1 0.0 0
20 40 60 80 100 120 Dose %
Dow-Rlsponoo Plot 0.8 0.7 0.6 S0.5 0 0.4 o 0.3 0.2 0.1 0
sqnO2-26-02.xis
Environmental Testing Solutions, LLC Statistical Analyses Used fhr PMSD calktisn oaly.
Larval Fbh Growth and Survival Ted-7 Day Growth Start Da*
2=2&02 Tet ID, PpFRCR Sanple aD TVA SQN, Outfall 101 - Nontreated End Doc 3/%i02 Lab D:
ETS-Env. Tesing Solutions SapT Tpe:
DMP-Disch-ge Monitoring Report Sample Date Protocol:
EPAF 91-EPA Fr*dbwur Test Speciea:
PP-Pimephaltz 1110e11 Comments:
Cone-%
1 2
3 4
D-Cotrol 0.7022 0.6460 0.6180 0.4940 10.98 0.5552 0.5972 0.6105 0.3299 22 05795 05514 0.5223 0.5353 43.9 05713 0.5299 0.6263 0.5426 72 05794 0.6186 0.6930 0.5601 100 0.6849 0.6259 0.6519 0.3868 Transform: Unbansformed 1-Tailed Conc-%
Mean N-Mean Mean MiR Max CV%
N t-Sat Critical MSD D-Conttol 0.6151 1.0000 0.6151 0.4940 0.7022 14.302 4
10.98 0.5232 0.8507 0a5232 0.3299 0.6105 25.040 4
[.434 2.410 0.1544 22 0-5471 0.8896 0-5471 0.5223 0.5795 4505 4
1.060 2.410 0.1544 43.9 0.5675 0.9227 0.5675 0-5299 0.6263 7.548 4
0.742 2.410 0.1544 72 0.6128 0.9963 0.6128 0.5601 0.6930 9.589 4
0.036 2.410 0.1544 o00 05874 0.9550 0.5874 0.3868 0.6849 23.133 4
0.432 2.410
- 0. [544 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) 0.87742573 0.884
-1.24633217 1.425134333 Bartletfs Test indicates equal variances (p = 0. 10) 9.240524292 15.08631706 Hypothesis Test (1-tail. 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetfts Test 10t
>100 I
0.154409948 0.251052676 0.005359172 0.008210062 0.663285613 5, 18 Dose-Response Plot 0.8 0.7
- 0. 6 0.5 1-tail, 0.05 level
- 0. 0.4 of significance o
0.3 0.2 0.1 0
sqnO2-26-02.xls
Environmental Testing Solutions, LLC Statistical Analyses Larval Mld GrowtM and Swvtval Tat-7 Day Growth St= DaW 2/26&02 Ted ID PpFPRCR Sample ID:
TVA SQN. Inake -Nonneated nd Dar 3/5o Lab ID.
ES-Emv. Teting Soludong SaMp Tý:p DR.-Diachage Monhtoing Repo Sample Dat Pwtocol:
IAF 91-EPA Freshwa Test Species:
PP-l'mePlas P-3-dM C o i n n m :
i-p ed Cow-%
jCOMa1 100 2
3 0.7022 0.6460 0.6180 0.5365 0.6817 0.5245 4
0.4940 0.5910 Transform: Untranwformed 1-Tailed Conc-%
Mean N-Mean Mean Mi Max CV%
N t-aSlt Critical MSD D-Contol 0.6151 1.0000 0.6151 0.4940 0.7022 14.302 4
100 0.5834 0.9486 0.5834 0.5245 0.6817 12.276 4
0.558 1.943 0.1102 Auxiliary Tests Statistic Critical Skew Xurt Shapiro-Wiks Test indicates normal dist.ibution (p> 0.01) 0.962760925 0.749
-204880953
-0.576806113 F-Test indicates equal variances (p = 0.74) 1.508488178 47.46834564 Hypothesis Test (I-tail 0.05)
Homoscedastic t Test indicates so sigoifcan differesces Dose-Response Plot 0.8, 0.7, 0.6.
Oz, 0 0.4.
a 0.3 0.2.
0.1 0 1 1-tail, 0.05 level of significance S
9 0
sqnO2-26-02.xls 4
0.4940 0.5910
Environmental Testing Solutions. LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1002.0)
Speai I
C o h V Species: Ceriodaphnia dubia T a Date Time Analyst Test start C'k2--26.0.
Is 1Z
.. I tet end O-0
-oz0t02 IS Client:rVW\\
5eja%)0,Mk
&r""je~ P~r Facility:
AMdAJx-eA-et N PD E S #:
" ' - o _~
- Dilution prpartion information:
Comments:
MHS batch:
/-a.OZ
!o
-zj.._
i.-.
prep Ib~
2t q.,_
- 17.
Io Ef,,
uvo*hme (mL)
Diluast vohmme~L 1L.71S c'l*46 2
3~
0 Test organism information:
Test informatown:
Organism age:
'-7(A.- Aoo U r aRandomizing template:
&Ue Date and times organisms C.2-z-O 1
Oh1o `o 1 Z3G Incubator number:
were born between:
2 Organism source:
Oz- "q-z A**6 YCT batch:
A19" tIQ-2-Z Transfer bowl information:
pH = *i Temperature = ?sq.q Selenastrum batch: AS6 zZ-7-"
CONTROL Survival and Reproduction Data Replicate number Day 1 1 2
3 4
5 6
7 8
9 10 1
Young produced 10 Q
___ 0 0
G (D_.
Adult mortality L_.
L_
L.
2 Young produced p
n G
3 Young P ouced-
- 0.
C) 1&jj A/
1 4 z
Adult mortality L ILL L._
L L
4 Young produced
-7 Adult mortality I
L L-L.
U I_
L'- I L
6 Young produced [1 J2 L
K Adult mortality [
~
~ u_ _
LLU 6
Young Produced 1211 4 2-iIL Fid Adult Mortality
=.
X for Broods Calculations and data reviewed:
Test Renewal, Feeding, and Incubator Location Infornation Mon Tue Wed Thu Fri Sat Sun Time szz
'-A Ism Shelf ILocation L
b A-I Control information:
Acpe criteria Summary of test endpoints:
% of Male Adults.
20%
7-day LC50 "It O0o 7
%Adults having 3" Broods:
/
.80%
NOEC 1OK
% Mortality:
- 520%
LOEC
> 100 Mean Offspring/Female:
5.'
- 5.
Ž15.0 offspring/fetmae ChV
> 1_007.
% CV:
91%
< 4o.0 %
IC25
> t_____
Pso* I rW*
Environmental Testing Solutions. LLC Page 2 of 8 Species: Ceriodaphnia dubia Client: -&SA S_& tee$
Date:
0Z-2(o-oZ.
CONCENTRATION:
WAS%
Survival and Reproduction Data
~
number_
Day 1
2 3
4 5
6 7
3 9
10 Alartak
-L-L-
L-I--
L 1
Young produced n
C C) p n,
3 oudy n gproduced 20 737 z0 C>
0*Z Z25 2
Adult mortality 2
Young produced o
tr0ti AdulMmortaty Iiy L
Mea Youngrproducedl": G
=.'9 X-3 Young produced
/0 1
0 0
n 1
0 Aduhmrbtat L
L-U U
U U
4 You*ogproduced O
0
- 2) 1 I
Q 1___
Adlult mortality C
TJ U'
U U/*-
U L!,--
U
- 1--
I 35 Youg produced J
1 6
Young produced
/0 iz* 0
(
0 0 I3 IZt a
Total young produced 21 22 23 2z3 "2-V-
2Z2 a
2S, zq Final Adult Mo ty
=
Concentration:
I% Mortality:
% Reduction from Control:
oT.4/
Calculations and data reviewed:
CONCENTRATION:
27?_
Survival and Reproduction Data
__________Replicate number Day___
1 2
13 4
15 16 17 18 19 1 10 I
Young produced 61
__0C Adult mortality L4 L.. _
(
L fl YoungproJduced o I 2n Adultmortalty 4U-1U I' 3 Young produced iLL 11.3 14.1...1 Adl otaiy I
L-I I
=-L 4
Young produced o1014 1,-3 1
A IX1 1
Adult mortality I
U L
~mortaity L
L BI~ IKI~
H~
6 Yugpoue13 12-1 1111-1 IZ_____
6_____
0_____
1-5____
Adult mortality LLL U
67 dlmraiy~
V 4 1~I~I~I~
Young produced1_1kIi TF I
Z1 1_
0 Total young produced I ý 24 2 -
-2 Zt 2 5 S
i l
FbWa Adult Mortality
_____t I
C*
I Concentration:
% Mortality:
-0CA Mean OffspringFemale:
zq.z
% Reduction from Control:-3.q '
Calculations and data reviewed:
Environmental Testing Solutions. LLC Species: Ceriodaphnia dubia Client: -t-r(A Pe-CUoAi go Date:
02,. z,-o-..
CONCENTRATION:
Survival and Reproducin Data Day
! 1 3
4 5
6 7
8 9
10 1
Young produced 0
0 Adult mortal.ty 2
Yo~ungproduced 0 C)~6 r~
c Aduhtmortality L
-L C
W T L
L 3
Young produced 0
0 0
0
'10 0 1
A.
M' m
-L L
Adlmortadly C
4 Young produced 2
-5
.Q dL.
0 0
5 Young produced
- 2.,,
2 Adult mortality L
U L
L 6
Young produced L5 J2-1j 0
A, o
17 1,3 Autmortality L.
L-L L
\\.-
L-
- t.
7 Young produced 014 e)
O Total young produced Z 8' 3
2L zc.
-z' 27 Z 24 22 Z&
21 Final Adult Mortality L-C?
U7 t
~
W-?
C Concentration:
% Mortality:
I Mean Offspring/Female:
2S;.5
% Reduction from Control:
- '6.
Calculations and data reviewed:
CONCENTRATION:
and Reproducon Data Replicate number Day 21 3
4 6
7 8
9 10 I
Young produced 3
4 5
6 7'Q i
8 9
- 10.
0
()
Adult mortality
(_
U T
- t.
II
~t 5e-t~l 2
Young produced Adult mortality t..
3 Young produced
__AlI
=j-T3_
5
=
Adultmortality L-L L
To4a Young producd 3'
A4 2
I0
(,
0 31 e-)
Adult mortality I L-LI 45 Young prouce
/0
-7 16
?
-'i//'
0 I)1_/0 1AdultMortalt c
L.iL L
L I
i6 Young produced tITA1JZ T '11 I I-(p 45 Adult mortality TZ
=7 Young produced C.10 O
4 J7I
~
Total young produced j
So 26 Jo 26 260 Z7
-3)
~31 2-P Fina Adult Mortality C
C T
~~
t Concentration:
% MortalJtT':
0%
Mean Offspring/Female:
2f. 2
% Reduction from Control:
- 20.V, Calculations and data reviewed: 14ý F.aze 3 of 8
Page 4 of 8 E'rnlw.nmontul T'qting SnIntinnL LLC Species: Ceiodaphnia dubia Client: -A P
A-h Date:
)2-i,-O 2 CON CN ON: 4off Survival and Reproduction Data
_______number Da 1
2 3
4 5
6 7
8 9
110 t
5 1
You*gaproduced
,L C_...
._L C.-
0..
Ad.ft morta,,ty I_
L.
U.
L L.
U 2
Yo, producd C) tO d
1 1-I 0
'0 u mom.
L-L-
3 Youproduced 0
4 0 0 0
Adult Mortalty L-L.
L-L 4
Young produced 5
t Adltrb-t I
L-4 Young produced 7T-0
/0.
/-Ia
/C 0
/0f 1(.
Adult mortality
-utonfo Cnrl q.t*
7 Young produced C)
IC)
~
Q..L./~.L12.
Adulo TlationsL and Uaa eewd Totalyoungproduced
-z 2-1 Z1 z9 31 30 29 21 7-P Final Adult Mortality Concentration:
Mortality:
z')I Mean Offspring/Female:
.21
%Reduction from Control: - -24.717 Calculations and data reviewed:
CONCENTRATION: -=*7% jAJT7I ke-Survival and Reproduction Data I
Young produced
() 1 C.)10 1nn 1
2 Young produced 01 0
n 3
Young produced 4
51910113 Adult mortlt 4 Young produced 0D 0
D 0
4 5
Young produced q
/(1 12-.
12 z A-
/0 1
6
~Young produced
/./
/
I i~/1 1
1 Yugpouced fil0 0 0 0 Total young produced 2(1 2-7 2,
2-1 3o 2
31 1231 31 Flinal Adult Mortlt L-L L
c Concentration:
I% Mortality:
% Reduction from Control:
- XS7.
Calculations and data reviewed:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sequoyah Nuclear Plant, Nontreated February 26 - March 5, 2002
- oject number: 269 Reveiwed by.
concentration Replicate number Survival Average reproduction Coefficd of Peremt recduIon from
(%)
1 2
3 4
5 6
7 8
9 10
(%)
(offsprIngfemale)
()
Control 22 25 26 23 24 22 26 21 20 25 100 23.4 9.1 Not applicable 10.98%
21 22 23 23 23 24 22 23 28 24 100 23.3
&1 0.4 22%
26 24 24 23 24 21 25 25 23 27 100 24.2 7.0
-3.4 43.9%
28 23 26 26 29 22 24 22 28 27 100 25.5 10.2
-9.0 72%
26 30 26 30 26 28 27 30 31 28 100 2&2 6.9
-20.5 100%
31 32 27 29 28 31 30 29 27 28 100 29.2 6.0
-24.8 100% Intake 26 27 28 27 30 29 31 23 31 30 100 28.2 9.0
-20.5 ItfalL 101:
innett's MSD value:
ISD:
nAct nnett's MSD value:
2.063 8.8 1.809 7.7 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treaftent that can bedeclared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 13.0% ftom the control.
Lower PMSD bound deternined by USEPA ( 10 th percentile) = 1I%.
Upper PMSD bound determined by USEPA (90d percentile) = 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted fiom 33 laboratories for Cerlodaphnia reproduction in chronic reference toxicant tests.
EPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental
,tection Agency, Cincinnati, OH.
lient:
est dates:
Environmental Testing Solutions, LLC Statistical Analyses Sest D 2
2 TauID:
1 CdPqo Sasg4.D TVASQKOOfail 101-Noataed
&A DMa 3/=2 La M.
Es-Ev. TMtiM SOhitla Saqe0t0ps Ma-Di ge Matzitm-i Rq Smuh d
PDOa*:
EPAP 9l-OlA P mdwa.
Tat Spede:
OCeD q
da& a Cme-%
1 2
3 4
S 6
7 8
9 10 D.CO~l 22.000 25.0D0 26.000 23.000 24.W0 22.000 26.000 21.000 20.000 25.000 10.98 21.000 220 23.000 23.O00 23.000 24.000 22.000 23.000 281.000 24.".0 22 26.000 24.000 24.000 23.000 24.000 21.000 2.000 25.000 23.000 27.000 43.9 2&000 23.000 26.000 26.000 29.000 22.000 24.0W 22.0 21.000 27.000 72 26.000 30.00 26.000 30.000 26.000 20.000 27.A 30.000 31.000 2&000 t00 31.000 32.000 27.000 29.000 21.000 31.000 30.000 29.000 27.000 28.000 Transfrorm UnOUV -fOrmed Tailed 150te Come-%
Mme N-Mm Meam Mu Max CV/.
N t-Slut
- Czil, MSD Mmw N-Mm D-Conn 23.400 1.0000 23.400 20.000 26.(00 9.054 10 25.633 1.0000 10.98 23.300 0.9957 23.300 21.000 20.03) 8.105 10 0.111 2.287 2.063 25.633 1.0000 22 24200 1.0342 24.20 21.000 27.000 6.969 10
-0.877 2
2.063 25.633 1.0000 43.9 25.500 1.0897 25.500 22.000 29.000 10.168 10
-2.327 2.28M 2.063 25.633 1.0000 72 28.200 1.2051 28.20 26.000 31.000 6.852 10
-5320 2287 2.063 25.633 1.0000 100 29200 1.2479 29200 27.000 32.000 5.997 10
-6.428 2.287 2.063 25.633 1.0000 Auxilzary Tests Sutatit Crtieaw Skew Kurt Kolhogomv D Tea indicatm noma disnmbion (p >0.01) 0.84679538 1.035 0.130211394
-0.644187356 Bar-dedts Tet indicats equal vm*-wem (p - 0.801) 2.247287512 15.08631706 Hypotihesh Test (1-tb"l. 0.05)
NOEC LOEC ChV TU MSIU MSDp MSB MSE F-Prob df Dulnezs Teat 100
>100 1
2.06316906 0.088169618 63.62666667 4.07037037 1.7E-09 5,54 Unear tepsatlido (00 Resmnsps)
Point S
95% CL Skew IC05
>100 ICIO
>100 IC15
>100 1.0 IC20
>100 0.0 IC25
>100 0.8 IC40
>100 0.7 IC50
>100 0.6 0.5.
w 0.4 a-0.3 0.2 e 0.1 0.0
-0.1
-0.2
-0.3
-0.4 0
20 40 60 80 100 120 Dose %
Dose-Respose Plot 35 30 25 C
1-tail, 0.05 level 20 of significance 10 5
10 sqnO2-26-02.xfs
Environmental Testing Solutions, LLC Statistical Analyses ce ilodagiai Sun'hviluad Rirodwedgo Te ft-Rq-od Z
s Sat Daer 2(2 Teat 1D CdFRCR SipM IVl WA SQN, hnaka - Nonreueed End Dauz 3/5102 Lab ID:
MawS-Fa.
Testing Solutions Sample Type DMR-Dischwge M*ntoring Rcp SAp Dam Protocol EPAF 9 I-EPA Freshwatw Test Species:
CD-Ci dubia Comments:
Come-%
1 2
3 4
5 6
7 9
10 0-Comnn 22.000 25.000 26.000 23.000 24.000 22.000 26.000 21.000 20.000 2.00 100 26.000 27.000 28.000 27.000 30.000 29.000 31.000 23.000 31.000 30.000 Transform: Untramernned 1-Taied Cone-%
Mean N-Mean Mesa MIM Max CV%
N t-stat Critical MSD D-Control 23.400 1.0000 23.400 20.000 26.000 9.054 10 I00 28.200 1.2051 28.200 23.000 31.000 8.971 10
-4.600 1.734 1.809 Auxitiary Tests Statistic Crtical Skew Kurt Shapiro-Wilk's Test indicates notmaj dest'iburion (p > 0.01) 0.934985161 0168
-0.567916859
-0.329037901 F-Test indicates equal variances (p = 0.61) 1.425742626 6.54108572 Hypothesis Test (t-Utl, 0.05)
Homoscedastc t Test indicates no signifcant differences Dose-Response Plot 35 302 25 0 20
- 15.
10 5-0 1 1-tail, 0.05 level of significance sqnO2-26-02.xls
Chronic Whole Effluent Toxicity Test (EPAI600/4-91/002 Method 1000.0)
Species: Pimephales promelas Client: 7rA Sao AA e
(?A,%rr Facility:
V-- ri
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Comments:
M batch:
0-2-t.1OZ 102-2-'LZ.
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GLUe Date and times organisms Gz.ZS'OZ i1O TO tSco t(£*
Incubator number:
were born between:
153o 1-7 G:Sr Organism source:
A 6S 8ATCM 02-zi-O2.
Artemia lot number:
6&tz Transfer bowl information:
pH =
Temperature =
Oven temperature:
t.o'0 7-3
.q t42 Drying time:
- 5 Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 0~a IZ2~O ASO I'7 2
02-zf-o2.
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Aceptance criteria Summary of test endpoints:
% Mortality:
I o
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Date:
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Surtival and Growth Data Day CONTROL O,_
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Comments:
Environmental Testing Solutions, LLC Species: Pimephales promeias Client: -WA -- Se DuoVa4 oi --rleATeb Day 0
1 2
3 4
5 6
7 A - Pan weight (mng)
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Larvae weight (ag) A A-B aq M
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0)22-.*p-Comments:
Environmental Testin2 Solutions, LLC Species: Pimephales promels Client: -frVA - S*un.wq,4 VV-rr'e*ere-b Date:
O2-goz.
Surival and Growth Data Day 1o06%
mrA Ke.-
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B - Pan +Larvae weight (mg 4,~
Larvaeweight(mg)-A-B B
.0 I[o Calculations and data review Comments:
skU 5
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Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pinmephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sequoyah Nuclear Plant, UV Treated February 26 - March 5, 2002 269 Rcvdweod by:__________
Dunnett's MSD value:
PMSD:
Dunnett's MSD value:
PMSD:
0.1109 23.3 0.0456 9.6 MSD Minimum Significant Difference PMSD -
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and traetmnt that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for EFnionmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.5% from the control (detennined through Lower PMSD bound determined by USEPA (10th percentile) = 9.40/.
Upper PMSD bound determined by USEPA (90th percentile) = 350/.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimcphales growth in chionic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R4-0-003. US Enavironincitaj Protection Agency, Cincinnati, OIl.
Client:
Teat dates:
Project number:
Coe.t,-raton (%)
dat lumberof FInalnumbroflavae A - Pan weight B = Pan + Larvae Laae wlht (rag)
Weight/ IIital aumb-Mean sarival (m&
Ce I larvae (nwj weight (nag)
A - B of Iarva (Mg)
(%)
()
e
(%)
A 10 10 14.787 20.040 5.253 0.5253 Control B
10 10 14.820 19.580 4.760 0.4760 C
10 10 14.645 19.360 4.715 0.4715 D
10 10 14.993 19.320 4.327 0.4327 E
I0 10 14.696 19.630 4.934 0.4934 10.98%
F 10 10 15.007 20.210 5.203 0.5203 G
10 10 15.048 20.640 5.592 0.5592 II 10 10 14.841 20.680 5.839 0.5839 1
10 1 0 14.657 19.530 4.873 0.4873 22%
J 10 10 14.687 19.240 4.553 0.4553 K
10 10 14.682 19.540 4.858 0.4858 L
10 9
14.917 21.130 6.213 0.6213 M
10 10 15.057 22.220 7.163 0.7163 43.9%
N 10 10 14.754 19.680 4.926 0.4926 100.0 0.5654 1812
-187 O
10 10 14.886 19.990 5.104 0.5104 P
10 10 14.977 20.400 5.423 0.5423 O
10 10 14.714 20.820 6.106 0.6106 72%
R 10 10 14.662 20,450 5.788 0.5788 100.0 0.5395 12.4
-13.3 S
10 10 14.866 19.520 4.654 0.4654 T
10 10 14.808 19.840 5.032 0.5032 U
10 10 15.016 20.030 5.014 0.5014 100%
V 10 10 15.031 20.140 5.109 0.5109 W
10 10 15.010 20.170 5.160 0.5160 X
10 10 14.949 20.900 5.951 0.5951 Y
10 10 15.271 20.850 5.579 0.5579 100% Intake Z
10 10 14.895 20.760 5.865 0.5865 100.0 0.5516 5.0 AA 10 10 15.025 20.430 5.405 0.5405 BB 10 10 14.654 19.870 5.216 0.5216
Environmental Testing Solutions, LLC Statistical Analyses Latyl PS& rmwIa" SarYWu Tal-? Day GfOw~k Stat* D
=
2 PPFRC Sum*
I TVASWK OuCM 101.- UV Treas
&ed Dow Y=.e Lab In:
M&S-v. TutalnSotueorw SasIl"Type:
M~~a4XwuepM-Uimiqg Ppo
&Wq1eu PtOcOL.
EPAF 91-EPA Fruswatw Tai Sped=
PP-"ieaep Mogid t
D,.Couwze 0.5253 0.4760 0.4715 0432*7
)55 0.43Z7 M0.98 0.4934 05203 0,5592 0.5839 22 0.4873 0.4553 0.4858 0.6213 43.9 0.7163 0.4926 0.5104 0.5423 72 0.6106 0.5 0.4654 0.5032 t0o 0.5014 0.53109 0.5160 0.5953 Thuifwm Uasmj9,~
-Tailed Iooi COM-%
Mma N-Mem Man Mb Ma--
NT*41-'
CziIwal MSD Mean N-M a D-rol I
.~
a
.r;73;;
"em Z;:
- .Z 40z4453 7.971 4
0-5273 1.60-0 10.98 0.5392 1.1319 0392 0.4934 0.5039 7.459 4
-1.365 2.410 0.1109 0.5273 1.0000 22 0.5124 1.0757 0.5124 0.4553 0.6213 14.454 4
0.783 2.410 0.1109 0.5273 1.0000 43.9 0.5654 1.1869 0.5654 0.4926 0.7163 18.161 4
-1.934 2.410 0.1109 0.5273 1.0000 72 0.5395 1.1325 0.5395 0.4654 0.6106 12.392 4
-1.371 2.410 0.1109 0.5273 1.0000 100 0.5309
.1144 0.5309 0.5034 0.59531 8.149 4
-1.183 2.4t0 0.1109 0.5273 3.0000 AaSItlatyrTeats "h----i----'t-ca Skew Knr A-dfa Tau Shs Teat indi
-e1a vf'ists]
o(p 0.50) 0.9129 209"0.884 1.033702897 0
4.345994949 15.08631706 Dyoleu el(-al.00)NE OC CV TU MSI~u MSDp MSB MSE F-Prob f
Dunne~sTest
-00
>100 l
0.1t094044 02.32884626 0.003655427 0.004230141 0.5242ý3785 5.15 Point SD 95% C LExp)
SkLte-be-w)
IC05
~>100 Se ICIO
>100 IC15
>100 IC20
>100 1.0 IC25
>1300 089.
IC40
>1300 0.8 ICSO
>1>00 0.7 0.6 S0.5 S0.4 o 0.3 w 0.2 0.1 0.0,
-0.1 L.
-0.2
-0.3 0
20 40 60 80 100 120 Dose %
0.8 0.7 0.6 0.5 0 0.4 o0.3 0.2 0.1 0
0.8
,N 1-tail, 0.05 level of significance sqnO2-26-02.xls
Environmental Testing Solutions, LLC Statistical Analyses Lra IhGownh amd Sorvraj Tal-7 Day Growth 2w et
/2M~
a D
9RJ Satipte m.
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Sml Datm Protocol EPA 91-EPA Preahwater Ten Speies:
PP-l ephl-ies poo*asa.
Comm-%
1 2
3 4
D-Cosmu 0.5253 0.4760 0.4715 0.4327 100 0.5579 0.5865 0.5405 0.5216 Tranform: Utransonred i-Tailed Cone-%
Mean N-Mean*
Max N
t-Stat Critical MSD D-Cos'ol 0.4764 1.0000 0.4764 0.4327 0.5253 7.971 4
100 0-5516 1.1580 0_5516 0.5216 0.5865 4.999 4
-3207 1.943 0.0456 Auxiliary Tests Statistic Critical Skew Kart ShapioF-Wi 'sTest indicates e
uoa al r
eks 0.61
.O1) 0.965459824 0.749 0.314736868
-0.404384508 FyTest indicates equTe varaues (p( 0.61 1.89620471 47.46834564 Hypothesis Test (Iti.0.85)
Hornoscedassc t Test indicates so signficast differeces Dose-Respoose Plot 0.7 o.0.4 S0.3 0
1-tail, 0.05 level of significance sqn_02-26-02.xrls
Species: Ceriodaphnia dubia and Pimephales promelas Environmental Testing Solutions, LLC Summary of Chemical Analyses Client:
Seqa Test dates:
Febr Project number:
269 oyah Nuclear Plant Non-Treated uary 26-March 05, 2002 Pimephales promelas C
oldahnida b"k V.J1 Z*.31 "IU
'I,
'V 0.2 24.2 2
4 0.I
.17T 7.907 8.101 8.021 0.08 24.3 25.j 2.8!
0.3 244J 5.3 24.81
- 24. 2471 4.!
0 2421 4'1" 2441 Overall Temperature (*C)
(including all concentrations for initial and final tempcraturcs) 26Q0 0.2 24.2 25.3 4+6 41.2 25.2 24.6 C4,?- o*,03-d2-
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPAI600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Daily Chemical Analyses Client:
Sequoni Test dates:
Februa Project number:
269 ah Nuclear Plant Non-Treated ry 26-March 05, 2002 I Iybu I 1.,ial N I
- 111 7.93 1 +S011m,
L21 7.51 8.0 7.6
&I I PH (bU)
DO (mg/L)
Codutivity Ot mhol/em)
Alkalinity (mg/L CaCO.O Hardness (mg/L CaCO%)
Temperature (C) uay u Initial I
Final
)!!
(S)48.-
24.41 14.41 Day I1 Day 2 I
I iaul I
Initial I
- 081 7.861 8.09 8.0 7.5 7.6 86 Of1 (SUi) 8.111 7.98 8.08 7.9 8.12 7.90 8.10 7.56 8.091 7.52 8.11 7.931 8.10 8.00 DO (mg1L) 8.0 7.9 80 74 7.8 7.7 7.9 7.1 8.1 6.5 8.1 7.5 8.0 7.4, 10.98%
Cnnduclivltz (Jsmboalcns) 309.
32.2 304 308 302 304 310~
Temperature CC) 24.6 24.4 24.8 247 25.0 24.3 24.9 24.6 24.5 24.2 24.4 24.4 24.7 24.2 PH (SUJ) 8.11 7.95 8.07 77 12 7.90 81 7.57 8.06 70u 8.07 7.91 8.09 8.00 22%
DOO(mgIL) 8.0 7.9 8.0 7.491 7.71 8.0 7.2 8.2 6.4 8.2 7.5 8.0 7A4 Conductivity (jsmhos/cm) 297 307 290 294 289 292 29z
________Temperature CC) 24.7 24.4 24.81 24.7 24.9 243.29 24.61 24.5 24.3 24.4 24.4 24.7 24.2 PH (SUI) 8.08 7.92 8.031 7.77 8.07 78 8.07 7.561 7.98 7.49 8.00 7.901 8.07 7.95__
43.%
O (nsgL) 8.0 7.9 8.0 7.3 8.0 77 8.0 72 8.2 6.5 8.3 7.51 8.0 7.5 Conductivity (jsmbos/crn) 26.4 272 6
263 260 261 270
_________Temperature (C) 24.7 24.4 25.01 24.7 25.0 2
24.9 24.6 24.5242 24.5 24.41 24.8 24.2 Z.
- t. 247 20 23 27 246I 243 24 2 2432 PH (SU) 8m
~
c n
~
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o, o
03 786 7991 7
803 1
3.031 7.95 DO (MgtL) 8.11 7.9 8.0 8.1 8.2 8.41 6.41 8.51 7.61
.9.0 7T 802 788 T46 790 Conductivity (Umhol/cm) 222 225 220 223 224 222 228ý Temperatre C C)
PH (SU)
DO (mg/L)
Conductivity (,imbos/cm)
Alkalinity (mg/L CaCO%)
Hardness (mg/L CaCO,)
rotal Residual Chlorine (mg/L)
Temperature (CC)
I.pH (SU)
DO (mg/L)
Conductivity (Jsmhodcm)
Alkalinity (mg/L CaCOJ) 24.81 24.4 25.0 25.1 25.0 24.6 24.5 7.971 7.80 7.92 7.95 7.951 7.48 7.77 8.3 7.7 8.2 8.2 8.3 7.4 8.5 177 178 183 55 54 56 66 70
<0.10 25.01 24.41 25.21 24.71 25.21 24.3 25.01 24.61 24.5 7.961 8.01 7.881 7.711 7.921 7.771 7.911
- 7.
7.76 8.31 7.81 8.2 7.51 9.21 7.7 8.4 8.5 177 176 175 5
59 6
76
<0 10
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- 24.
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100%
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Total Residual Chlorine (mg/L)
Temperature C'C)
I I
I A
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Jemperature (*C)
I 24.3 24 7 9d
- I 94 +*
94 9 1.70 0.*1.) I 1,*0 fi.UL I "J'* I IKR 7Q(I D [%'* I FIp
- r i
ronmental Testing Solutions, LLC Species: Pimephales promelas Client: "*JA
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Full-strength Chemishry:
Parameter S
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1 2
3 PH
- 7.1 T
(s.u.)
DO Conductivity (11 10 (r hos/cm)
Alkalinity S.5 i5ka (ma CaCO3IL)
Hardness
(,
-10 (mg CaCOJ/L)
<0.0
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(
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Collection start date Collection end time lqi I*
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upon receipt 0qC 02C
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- 42oOz, Date:
nz-Zzf,-0.7 S.
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0?-If-Oz 0,2,7 364 T'.09 J M 641 I
- 10 05 10 1
V) 0 0
t~j 40 VI 0
vt I,
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vironmental Testing Solutions, LLC Species: Pimephales promelas Client: "'A
- e AJ Date:
OZ-Z(.O27_.
Environmental Testing Solutionsa TjrC Page 8s o ~s Species: Pimephalespromelas Client:
-WA SgMWAAP POtjowf-eA-reb Date:
O-.-z.
Concentrton Parameter3456 CONTROL pH(S.U.)
74-10 tWo f-1
.41 to1 11-1 1
- 6.
7I.V
.i
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DO' Cond8uct1ivit1y75
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~
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Temperature 24A~
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8.0
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1
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Z-2t4.4 pH (S.U.)
9,0
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I a7.0 DO (mg/)
z V
Temperature 2--
Z1 24.1 V. Z,~
/(
C.
______p (SU) 1 l
79 intilI Final j nta IFinal IInitial IFinal I
Tný*4 11I Vlnq.I I
S4 I
=**
U
.---
Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubia Daily Chemical Analyses Client:
Sequoyah Nuclear Plant Non-Treated Test dates:
February 26-March 05, 2002 Project number: 269 Rev1ewed by; Concentration Parameter I
Day 0 D
ay I
Day 2 Day 3 Day 4 Day S Day 6 pH (SU) 8.09 8.11 8.08 8.17 8.09 8.08 8.10 8.07 8.10 8.08 8.11 8.16 8.11 8.15 DO (ag/L) 8.0 8.1 8.0 8.2 7.6 8.0 7.9 8.1 8.0 8.1 8
8.0 8.0 8.1 Coondlt0 lvi-(IA tit m
2 1
2m Control Alkalinity (mg/L CaCO3)
Hardness (mg/L CaCO3)
I.
65 86 T_______
emperature (*C) 24.3 24.5 24.6
- 24.
24.7 24.2 1 24.61 24.51 24.31 24.61 24.3 24524.71 24.3 PH (So) 8.11 8.14 8.08 8.8 8.12 8._0 8.10 8.09 8.091 8.07 8.11 8.78.10 8.17 109% DO (mig/L) 8.0 8.1 8.0 82 7.8 8.0 7.9 8.1 8.1 8.2 8.1 808.0 8.0 109% Conductivity (pmlsos/cm) 309 322 304 308 302 304 310 Temperature (*C) 24.5 24.5 24.7 244 24.9
- 24.2 24.7 24.5 24.5 24.6
- 24.
45 24.7 24.3 PH (SU) 8.11.
8.13 8.07
.9 8.12 8.9 8.10 8.09 8.06 8.07 8.07 8.48.09 8.14 DO (mngL) 8.0 8.1 8.0 8.2 7.9 8.0 8.0, 8.1.
8.2, 8.2, 8.2
.08.0 8.0 22%
Conductivity (jsmhos/cm) 297 307 290 294 289M 292 298 Temperature (*C) 24.5 24.5 24.7 24.4 24.9 24.2 24.7 24.512.
24.61 24.4 24.5 24.7 24.3 PH (SU) 8.08 8.12 8.03 8.20 8.07 8.12 8.07 8.0 7.981 8.06 8.00 8.14 8.07 8.14 4.%
DO (mgfL) 8.0.
8.1, 8.0 8.2 8.0 8.2 8.0 828.2 8.2.
8.3 8.1 8.0 8.1 Conductivity (pmhos/cm) 264 272 261 263 260 21270
_______Temperature (C) 24.8 24.5 24.9 24.4 25.0 24.2 24.7 24.5 24.6 24.6 24.4 24.5 24.6 24.3 pH (SU 8.03 8.10 7.98 8.14 8.03 8.08 8.02 8.04 7.88 7.99 7.90 8.07 8.03 8.12 DO (mg/L) 8.1 8.2 8.0 8.3 8.1 8.3 8.2 8.3 8.4 8.0 8.5 8.2 8.0 8.1 72%
Conductivity (jsmhos/cm) 222 225___
22022
___24_2222
________Temperature (C) 24.9 24.5 24.9 24.4 25.0 24.2 24.8 24.5 24.61 24.61 24.ý5 24.5 24.6 24.3 PH1 (SU) 7.97 7.97 7.92 8.10 7.951 8.03 7.95 7.90 7.771 7.96 7.751 8.061 7.99 8.10 DO (mg/L) 8.31 8.2, 8.2 8.4 8.21 8.31 8.31 8.31 8.51 8.01 8.61....
8.2 8.1 82 Conductivity (pmihos/cm) 177 100%
Alkalinity (mg/L CaCO3)56 Hardness (sng/L CaCO3) 66=
Total Residual Chlorine (mg/L)
<0.1010 T_______'emperature (C) 24.91 24.51 25.01 24.41 25.0 24.21 24.7
- 24.
24.61 24.61 24.71 24.51 24.71 24.31 PH1 (SU) 7.96 8.03 7.88 8.121 7.921 8.061 7.911 8.2 7.761 7.991 7.731 8.221 7.991 8.1 DO (mg/L) 8.31 8.21 8.21
- 8.
8.2 8.1 8.41
- 8.
8.51 8.01
. 8.6 4 -.
Conductivity (pinkos/cm) 177 17517 100% Intake Alkalinity (mg/L C*CO3) 545 Hardness (mg/L CaCO.3) 68M7 Total Residual Chlorine (mg/L)
<0.109 emperature (C) 25.31 24.51 24.81 24.41 24.9 24.2 24.612. 44
- 2.
24.91 24.51 24.61 24.3
- vu*N*
v Jij, lima
Environmental Testin! Solutions. LLC Pages Ct Species: Ceriodaphnia dubia Client: -TiJA Q~
iJ a%4 Ai& F:PLL. 10 tAb3rj1-eAreb Date:
OZ-ZG-O2.
Full-strength Chemistry:
Parameter Sample Number:
Control Batch:___
1 2
3 dZ-If-oz.
02.-AdtZ pH
.O DO (mng/L)_
Conductivity
.i-1e I
(pimhos/cm)
(mg CaCO3L c!.L Q__
Hardness (mg CaCO 3/L) 4.'.,
Chlorine 0
.0
-C.1 Collection start date Collection end time Grab or Composite 7S-tW'J'-
ThI: 4Ct?..
Mra (duration)
"evseorp-Coi4VSrr4 C0ib Temperature C0C) 0.91 "C 62 I:
0-(.%C.
upon receipt I____ ____
Physical eALe em
(
characteristics
'4E.LLO) -k %
ea%
C~ejV.
C-LeA4g C-LeA4.
Dates sample used 62x o z-'zft,0oi.
s-o2-o Q.zZ1O0z qf-6k0L 03-13a2.
ETS Project and 261 2 Sample numbers 02M6JO.61I 020271.0all?103OZ.Ol Environmental Testinz Solutions. LLC Page 5 of 8 xrelb,10 -64 I ?,.ýo
ý
Envionmnta Tetiu Soutins.LLCPage 6of 8 Species: Ceriodaphnia diibia KomkeATe1b Fullstrength Chemiviry:
Date: (.Z*2 Parameter Sample Number
___Control Batch:___
12 3
6ZiR.f-Q7 02.zG-OZ pH
.A-71 DO Conductivity 1I73l0 3114 (gmnhos/cm)
Ailcalinity 5A19(96 (mg CaCO3VL)
N_
1_
H-ardiness
~(mg CaCO3/L)
-760 Chlorine 0J Collection start date 07407 z(at 2ý2-?
Collection end time NM 0 q3-s i BUS Grab or Composite MR6 Z3.&o0J' t3-Si000 (duration) cowifrre. cojwre Co~ros Mre.
Temperature (TC) 0.1w C.
upon receipt_____
Physical eAc.
eAC k~
characteristics
'4eu..ow J04Wa~
4iPLLUw
%:uArts*
Dates sample used OZ2.--
ovz..fo?
O302O6Z ETS Project and Vol9 2G?/6 Sample numbers lmz&d OOwI.l 0
2.
Environmental Testing Solutions, UC
EnirnenalTstn Souin.LCPae7o Species: Ceriodaphnia dubia Client:
"TVA SedQcHoA Daily Chemistry:
Date:
7.,zi6,o2
-Da Concentration Parameter 0
CONTROL pH(S.U.)
itCA.
Conductivity IMAS (gmhosknm) iSZ Temperature
?q.3
¢Oc)
-q3
-q*
z b 24.4 z
7-4.
24, 7 pH (S.U.)
- r.
Is. 1 8.10 DO (rag/L)
,L Conductivity B
j37 (jimhos/km)I Temperature CC) z2.1 q,
- v. A
- z p) (S.U.)
-.f 417.
3.0 DO (mg/L)
O
'*,1 2Z*
Conductivity (pmhos/cm)
Z Temperature ¢c) zq,5
- .s"
~~z4.1 z,'
q=
a.*
pH (S.U.)
(*0°
- .IZ.
8.03 g.2 d0 o" 6,tT.
4:.q '
Conductivity Temperature
~i(
pH (S.U.)
8.f
&0 1
.0-3 o9.
A
&. O7 8.1z DO (mg/L) 9-9 2 "1z '*
Conductivity
( 1 (plos/cm)
Temperature (0C)
PI,(oc U) 9.0-9q.10
'7 -.11 S.q z(f.o 05.e DO (sgu.)
S.. 7-q l'*
.t 7.q o
-1 Conductivity L1..1 (Vixhos/cm) 21 2
Z Temperature 2
7s.
Z (OC)
DO (mg/L) z.2.
- 8.
Conductivity
/NT(ýunzhoskm)
/(ýo Temperature 2q 454q
-Nq.z
_c)_*._
_z__.(C)
Initial H
Final Initial Final Initial Final Environmental Testing Solutions, LLC Page 7 ofr
Environmental Testin! Solutions, LLC Page of 5 Species: Ceriodaphnia dubia Client:
'TVA cSp&a'lf Date:
02-26-0Z.
Concentration Parameter34
- ý.t4 CONTROL pH (S.U.)
6.10 T.0 10I 6.06 13I4
.11
'81 -1 1.
DO 1.9~ 13; 8.o S-1 Conductivity HAS p (phS.U.)
.0 ll Temperature 74 0
-1A. 4.
3 24.~ 6zu -2.
T4~ 5 741
'2q.3 pH (S.U.)
6-o R.04 0.0~-4 8.O11
.r
.1 DO (mL 21.9 A
a -I2 1.2 &.0 J).0 7
2 Conductivity t
(jirhos/cm)
Temperature 7.4415 2q. 6~
2q. 5 4
DO (mg/L)
A'O97.,
27 Conductivity zl7 Temperature
-zq.-
zc4.5" 24A.
21.q
- _2q4,
-z.Lf3 PH (S.U.)
3 R.O 1.4 DO (mg/L) h.Z 0
9.70 6.3 e &0 A13.
q Conductivity ZO2(lZ1 (RP*uhS/CM) rZeb Temperature 7qqS
~
~
q~?.S
- 2.
4 PH (S.U.)
6.10..
-1.1 00.6-1
&2. 0 Ile.
DO (mg/L)
ýP.
.Z 8k5gZs M~c a&..-
0 Conductivity z
Z z
2
/Ah~
(Wnhos/CM) 3 Temperature 2zq.Qo q~
zq.5 z
.2qz.5
'I 2'/.(~z (0c)
Ibnfi tfia IFal l IInitial I Final I Initial g Final I Initial 11 b"'u'i.
Environmental Testine Solutions. LLC ftge 8 of 8
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Methods 1000.0)
Species: Pimephalespromelas Summary of Chemical Analyses Ckm:
Slqaaya N.dta. PMlW UV-Truad Tm dlun:
Fclooaq 26-iarch 05, 2002 Frojý -- 1-;b, 269 R.,,k-d b.. IA-
.*i-PimephaLks pronw1a 0
24.21 24.7 24.41 0.2
.051 7.51 7.961 7.821 0.16 D.2 6.6 7.9 7.41 04 24.3 25.21 24.91 0.3 0
24.21 24.61 24.4 0.1 05.50 7.94 7.81 0.16
)72 6.6 7.9 7.5 0.4
- 24.
"245 252 24.9 0.3 24.21 24.61 24.4 0.1 25 755 291 7.77 015 Orall T-qaraat-CC)
(induding al c
- waoa iwtiaraol. ail andp&W ara* a) 24.2 25.5 24.7 0.2
Alida vaz uuiaxI.iital I eUstilg 3oWuElons, LLU Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Daily Chemical Analyses Client:
Sequoyah Nuclear Plant UV-Treated Test dates:
February 26-March 05, 2002 Project number:
269 Concentration Parameter D
D D
D 4
Conal Alktiat Iniia Cnitia 9i Ofmpraure 8.C0 249 5843 2.40 47649 244 2 8.1 249 6745 2494.
24.524.
24.3 DO (ra./L) 8.5 8.0 8.0 77 82 6
7.6 7.9 7-5 2%
Conductivity (jzmhos/cm)29 Control Conductivity (phos/cm) 1 Alkalinity (mg/L CACO3 )
Hardness (mg/L CaCO
- 3) 6 Temperature (1C)245 243 2442
.1 2.1 2.241 2.1 2 -1 PH (SU)8.280248 2.3 10.98%
DO (rag!!,
8.0)
.7781 6.1
.9 -
Conductivity (pmhos/cm) 010
<0.10 3010 Temperature (C) 24.9 24.7 24.5 25.0 2544 PH_*
(su)
- 2.
244 24449 22%
DO (rag/L) 801 781 80
.1-f-
.80 Conductivity (jumhos/em) 80
.1 Ro
- 7.
T'emperature (10 9029 pH (SU) 80
.4
.3
.8 80
.1
.8232.
4.%
DO (mg/l,)
82 7
.579 43.%
8.0 7,a/L Conductivity ()Amhos/cm) 7 T em pe ratu re (°C )
2.1 2. 1 2.
4 6 2.
4 4 2.
4 5 2,
4 52 7 % DO (rag/L)8.8.
- 7.
8.T7 7873 83.680 76 Conductivity
-(pmihos/cm)2121222 Temperature (,C)2504A pll (SU) 79 D O ( m g / )8
.7
. 18 17 47 88 078
. 17 17 67
- 6.
Conductivity 0tmhos/cm)1701812 100% Itk Alkalinity (mig/L CaCO3) 5 Hardness (mg/L CaCO3) 6 Total Residual Chlorine (rag/L)
<.1 T e m pe r a t u r e ( *C )
2. 1 2.1 2.
5 42
. 1 2.
4 8 4 52 4 52 4
Revieed by: _-*J
Environmental Testing Solutions, LLC Page 5 018 Species: Pitep hales promelas client: -TIA-SeQ uo-YA 14 - O,.n-pAw 101 (1' - TreATeb Date:
6i2-2~-(z Full-strength Chtemistiy:
Parameter Sale Nuber:
Control Batch:_
1 2
3 O-~O.
2&3 pH
-. fs (S.U.)
1A5 I_
DO
-[A (In'g/L)__
Conductivity 11 C
?
-0 (Vimhos/cm)__
Z5O AkAlinity S
1 16 (mg CaCO3 /L)
I_
Hardness (mR CaCOJL) 10 170 Chlorine0.1 Collection start date Z202 2-.
z 2L207 Collection end time 14S O~e 15 Grab or Composite 25-810WC 2J$.$OiVe Za3kotz (duration) catousre.
ComjPOrrd ca$PsMl Temperature CC) 0..'(
1 le 010 06C.
upon receipt Physical M LPA... ?AL(
AL.
characteristics
'jCLLuý *L" cLLeA e-CiAA CLeA.
Dates sample used 07,;.&Oz.
0oi-*al 03-2-Zo ETS Project and 1Sample numbers LOZCoZ
.61 lozOZt. 0 (302050Z.OI Environmental Testing Solutions, LLC Page 5 of 8
Environmenta Testing Solutions, LLC Page 6 ot 8 Species: Pimepialespromelas Client: 7
-ed aq A
- I NTA*e UV/- r9e.'reb Full-strength Chemistry:
Parameter S
De Number:
S1 2
3 pH s.u.)
-7.q
, 1,9 DO (m9)
- 9.,.5 Conductivity (gmhos/cn) 1-7 1-78 Alkalinity (mg CaCOL/L) 59 Hardness
-t Chlorine I0I
.(
.~
Collection start date 10'- zq -o7 o2 -z*.*Z o-d z
-o2 _.9 O
Collection end time Gr-ab or C
ýomposite 25-14bat 23 AW*L "as.*.ovo?.
( d u r a ti o n )
O
- T.
Temperature C°C) upon receipt O.606C (6..
I.C, Physical eAL-
- AIO, A Le characteristics leaa Aeu-o "u
- t*
Dates sample used
- o.Z.-oZ 3.O 1-oz-V.o-oz 6 o6.,-Z 02-ZI2 4;-"Z.
OMq-OZ ETS Project and Z
U01 Sample numbers damrzzf.07.
MOO2W a20zoZ.
Date:
Z-oz.
Control Batch:
.*.co 0ot~,
Batch*. :....
o6:5o*
I
Environmental Testing Solutions, LLC Species: Piniephalespromelas Client:
TA - fqi~e.q0AA4 LNV -
eAeb Concentration IParameter
-PH (S.U.)
ConductiVit
.(limos/cm)
Temperaturme
("C) pH (S.U)
-DO (mFOL)
Conductivity
_ýýOs/cxn)
Temperature C)
PH (S.U.)
,DO (mg/L)
Conductivity
("3hos/crn)
Temperature C
pH (S DO ( rig/L)
Conductivity os/cm)
Temperature (0c)
_pH (S DO (mg/L)
Conductivity J
0S/CM)
Temperature CC) pH (S.U.)
DO (mg/L Conductivity DS/CM)
Temperature 0C Date:
~-2ad CONTROL
-PH (S.U.)
DO (mg/L)
R.. z Conductivity
(
)S/cm)
J'X Temperature (0c) 2r. 0 Inital I FnalI Th~i1It I
.i7ulu II
.5~A~
la Inam I
Page 7 of 8 72 %,
16001"K Initial 11 M_
I Initial I
Final
?4.3
.c- -Zq ci 2q.2 4'3 W~.0
/a)Tal
EnvionmetalTestn!
Slutons.LLCPa~e s atf s Species: Pimtep hales promeloi Client: -NJA-GeagcH-At4 Date: 02-Z6-07.
Da Concentration Parameter 3...
CONTROL pf S.U) 0.1 1
4G.
eoJ7 DO (mg(L)
-7,
- 1.
.1 f31 to 7.4 Conductivity
_ 13 Temperature
~
~
2 1
~
DO (mg/L) 1.71.
Z20.
Conductivity Temperature
- 71.
457 CC)O ZLS 21.1R~
-q zq.
zq 4'7 14 pH (S.U.)
-7Os i.1o AS.1
- 8.
2 l Conductivity~ghsc)Z9L Temperature Zsl U
N
~.
2.O 8
4 CC) z DO____
(
mgC
)-79' (4
Conductivity (Ipmhos/m 7)0 2-c 0(L Temperature
~2.
~C pH__
(S.C )
20.0-
-7.(.t 12 ý,F Conductivity I
Mr-~.
(jnuhos/cm) 2 4z Temperature
- 2.
4
~
~
q VZ DO________
(
3 Inta I Finl I Initil Final I
nta I
I'ni nta I
Final Enviroamental Testing Solutions, LLC
Enrvironmental Testing Solutions, LLC Page to5 PageIof Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accunmet Model 25 pH/Ion Meter Date analyzed
-Ztp4t Iodide reagent Acid reagent:
Slope:
Calibration:
0.10 r/L 1.00 msh Reference standard number IAS 0,IA Note:~ For samples with a residual chlorine of > 1.0 ing/l, the calibration range must be adjusted to bracket the chlorine levels of the sample..
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
%RS=MV/TVx 100 number (mng(L)
(mgiL)
(acceptable range - 90 to 110%)
I 0.50 o0 IZ Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = {(S - D) /[(S+D)/2]} x 100 number (acceptable range -
m 10%)
4/
Duplicate 11111 M
D4~o L
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine inumnber (1119P number Blank (should be=<0. 10mg/ýL)
Note: All samples were analyzed in excess of EPA recommended holding time (l5 minutes) unless otherwise noted.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
%/RSP=MV/TVx 100 number (mg/L)
(mg/L)
(acceptable range = 90 to 110%)
IN 0os 0.50 0,eu 10Z.19%
Reviewed by I Date reviewed I LN4;1. -OZ
Environmtental Testing Solutions, LLC Page
(^o Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter. Accumet Model 25 pH/Ion Meter Date analyzed 0-1.29.0Z Iodide reagent:
Acid reagent:
Slope:
Calibration:
[
0.10 MR/L 1.00 mg/L Reference standard number 0 h6ms tO o b
Note: For samples with a residual chlorine of > 1.0 mg/I-. the calibration range must be adjusted to bracke the chlorine levels ofthe samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
%RS=MV/TVx 100 number
((mV/L)
(mgL)
(acceptable range - 90 to 110%)
0.50 IO'I. q Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = {(S - D) /[(S+D)/2]} x 100 number (m1/L)
(acceptable rage 10%)
.OW222t.O1 SQ, OiTFALL IC I OI O jlrU ej*ft_,
S,0.0 7l DuplicateDID.
20 Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number mmbeL_
s1 Blank (shouldbe=<O.lOmg/L)F 4D.4 OOO-..
o~o2Zf. O2Z S-J iNr-A Ke 9oJL.C es
- .U LO cWLO Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otheswise noted.
Laboratory control standard:
Reference standard True value (TV)
Measured value (hMV
% RS =MV/TVi 100 number (sng(L)
(sng/L)
(acceptable range -90 to 110%/)
)r OU60.50 c.0-54q Reviewed by Date reviewed
Environmental Testing Solutions, LLC Page
("3 Page tL of LI Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter-Accumet Model 25 pH/Ion Meter Analyst L Z.
Date analyzed [p)5-Oj3J Iodide reagent:
Acid reagent:
Slope:
Calibration:
R EM M
M o.1o ;=
1.oo mR/
Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusto bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS = MV /I TV 100 number (mg(L)
(me/L)
(acceptable range -90 to 110%)
I MAx.SooS 0.50
- o.
0 q3. rib Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine numb er (m*/L)
Blank (shouldbe=<0.10mg/L) 7-.0,0096e5 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory con trot standard:
Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number (mg/L)
(mg/L)
(acceptable range - 90 to 110%)
I A 6o00o5 0.50 o.'1eI q(.7,v77t Reviewed by 4hI II Date reviewed IQ-oaL2
Page I _of Analyst Date analyzed [),5iU5_
ýraxt nonnality and mudltipie deter,
, - eotteut 0.1 - o.2 -o.*f*
oratory control standard.
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Titrate samples to pH = 4.50 S.U.
Reference True value Sample standard number (TV) volume Begin (Mcg CO (nil) m I 60. -o4,.
100 100 17.5 Duplicate sanwile nrecisi,..
Sample Alkalinity.
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO,/L)
{(S - D) /[(S+D)2]}
100 number ml ml ml ml (acceptable range =
J 1o%)
Oz-z.0o.
0Ht5-b8o I,
H7,0 D(
LZ-zL,-oZ pct Z9.C 3Dp li Oe D LaO.o Matrix spike recoverv:
Reference I Spike value I Sample i I
I I
i standard number (SV) volume Begin End Total Multiplier (mg CaCOaiL)
CaCO3/L mL Zq, ml ml ml
- 61)
~
~~
10029q 4
.1
'Tq 1
[1 Sample alkalinity (B)
Measured spike value (MV)
% R = MVI/ SV x 100 (mng CaCO3/L)
MV = A - B (acceptable range
(-g CaCO3fT..-
75 to 125%)
41
_0__
___4_
Sample measuremen Sample number to -z*.1-0 Z 03 -o i -O.
OZO 224a. 0 1 0zo ZZ(5, 0)
OZ0302.o 0 z 0 zzg. U-.
0 200,,04,01-ts:,m Sample ID
-,s,l* 2o 6SU. HIo 1.
Sample volume (ml)
Begin ml 140.1 0.0 Z(.W 3*,..
End ml ZI.D 3*.ze Total ml 3.0 5-S 6.0 Multiplier Alkalinity (me CaCO3JL)
(o So 55 5Lq Review ed I
Date rete ed pH of Delonized water
-4.5 S.U.
(.-4 Page L4I U6-04-0t-ryl tt.5 Date reviewed:
-cx5--a?.
Environmental Testing Sohlutions_
.r.t I
Multiplier q.fl I
I 7'
I Reviewed ]
I~~w l
I I
I
,m-
Page LIZ Environmental Testing Solutions, LLC Page Z~ of Z
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Titrate samples to pH = 4.50 S.U.
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)
TV~3..uz*
Iq 6~cq~oZ 3
"_3
.q 0.5 few..e Dtre:
3.9d0 31, 0 -,.
s**
o 3
-7,.
-+
":t 1,
t.3,) I
.3 zq-C)
Anls Date analyzed Date reviewed: I,;ý" 05" 07 Reviewed by:
Eknviron'mental Testing Solutions, LLC Page Lof t
Total Hardness (EPA Method 130.2)
Matrix: Water, MIDL =1.0 mg CaCO3/L Analyst p
Date analyzed Titrant normalitV and multiplier detemnatin:
Titrant N~ormality cheeck LBeegin End Total Normality (N) of XDTA pH Factor or Mu~ltipliler rerence standard mil ml ml
=0.21E
.(N x 5M00)I0ml sample number number (acIal age01-OOl
- NI 000
_C)~IT 0.0 10-0 lo.0 0- OW zo Laboratory control satadarik I1arwdnems (MWV) I %RS =MV /TV x 100 Reference True value Sample standard number (TV) volume Begin End Total Multipler (I g CaCO/L)
(acceptable range (in_ Ca)OmL
)
ml ml m
- 90 to 110%)
40 50 1o.0 1,.0 Du icafe sam levrecision:
=
Sample Hardness
"%PD Sample Sample ID volume Begin End Total Multiplier (mg CaCO/)
{(S -D) It(S+D)r211 x 100 mber (eptable range- : 10%)
number (m)
Int ml m
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SiehrnsA Reference I Spike value ISample Siehrns A
standard number (SV) volume Begin Ed TtlMlile mCCaL mCaCO3/L ml ml ml ml Sample hardness (B)
Measured spike value M )
% R =MV / I S x10 (gCi/)MV=
A-_B (a) cceptable ra:nge CaJuL
=75 to 125%)
ccm
Environmental Testing Solutions, LLC Page of Total Hardness (EPA Method 130.2) t'e Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst A
Date analyzed o.s Tifantand mfi~erd&ermh~atlof:
Begin End Total Normality (N) of EDTA pH Factor or Multiplier
[ L fe rence 1 st-aand a m l nil m l
-0.2/ 1 (N I SM 000Y50m l sam ple nr m bere n uebta er (wme ptab" range- 0.018 - 0.022) number number Laborato-- control s-anda Reference True value Sample Hardness (MV)
%.RS=MVTVx100 standard number (Tvolume Begin nd Total Multiplier (mg CaCO-)
(aptable r (mg CaCO/)
90"to.110/6) 40 50 Date reviewed Note: If > 1 5m of titrant is used, sample must be diluted.
Reviewed. by:"
Sequoyah Nuclear Plant Biomonitoring February 26 - March 05, 2002 Appendix D Reference Toxicant Test and Control Chart Information
ENVIRONMENTAL TESTING SOLUTIONS
- 1274 P.033/037 Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water Test number 1
2 3
4 5
6 7
8 9
10 11 12 13 14 15 16 17 18 19 20
'r'est date 7-day TC25 (g KCI/I.)
03-17-00 05-23-00 06-13-00 06-13-00 09-19-00 10-24-00 11-07-00 03-13-01 06-26-01 07-17-01 08-21-01 09-25-01 11-01-0]
11-06-01 11-27-01 12-12-01 01-04-02 02-05-02 02-13-02 02.26-02 0,68 0.69 0.72 0.70 0.66 0.77 0.65 0,69 0.53 0.74 0.62 0.60 0.71 0.66 0.57 0.63 0.49 0.61 0.65 0,56 Note:
7-d IC25 day 25% inhibition concentration. An estimation of the concentration ol potassium chloride that would cause a 25% reduction in Pirnephales growth for the test population.
CT = Central tendency (mean IC25).
S = Standard deviation of the 1C25 values.
S.1 Standard deviation corresponding to the the 7 5 th percentile CV.
SA.75 - 0.38, as dIcturmincd by USEPA for the mcthoxd znd e.ndpoint.
S.,_0-Standard deviation corresponding to the the 90"' percentile CV.
SA.90-- 0.45, as determined by the USEPA for the method and endpoint, CV = Coefficient of variation of the TC25 values.
USEPA. 2000. Undet-randing and Ao;Lumnting for Method Variability in Whole Effluent Toxicity Applications Under the National PoluLantL Di,4chury Elimination Program. EI'A-833-R.-b-0(03. US Envinronmental Protection Agency, Cincinntati, OH.
Organisms obhuined from Aquatic BioSysiems, Inc.
MAR-08-2002 10:00 P.033 S
'13&7 CT (g*L KCI) 0.68 0.70 0.70 0.69 0.70 0.70 0.69 0.68 0.68 0.68 0.67 0.67 0.67 0.67 0.66 0.65 0.65 0.65 0.65 0.01 0.03 0.02 0.03 0.04 0.04 0.04 0.06 0.06 0.06 0.06 0.06 0.06 0.06 0.06 0.07 0.07 0.07 0.07 CV 0.01 0.04 0.03 0.04 0.06 0.06 0.05 0.09 0.09 0.09 0.10 0.09 0.09 0.10 0.09 0.11 0.11 0.11 0.11 0.26 0.26 0.26 0.26 0.27 0.26 0.26 0.26 0.26 0.26 0,25 0.26 0.26 0.25 0.25 0.25 0,25 0.25 0.25 0.31 0.31 0.31 0.31 0.32 0.31 0.31 0.30 0.31 0.30 0.30 0.30 0.30 0.30 0.30 0.29 0.29 0.29 0.29 MAR,08.2002 10:48 8288628195 02-26-02.--k 8288628195
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S-- -
Central Tendency (mean IC25)
Control Limits (mean I125 + 2 Standard Deviations)
L'J 01 0
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MAR.UaO.002 Ia:49 U288628195 ENVIRONMENTAL TESTING SOLUTIONS
- 1274 P.036/037 Environmental Testing Solutions, LLC Precision of Endpotnt Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephales promelas using Moderately Hard Synthetic Water Test number Test date Control Control Mean Survival Growth C/o)
(rng/Awva.)
I 2
3 4
5 6
7 8
9 10 11 12 13 14 15 16 17 18 19 20 03-17-00 05-23-00 06-13-00 06-13-00 09-19-00 10-24-00 11-07-41 03-13-01 06-26-01 07-17-01 08-21-01 09-25-01 11-01-01 11-06-01 11-27-01 12-12-01 01-04-02 02-05-02 02-13-02 02-26-02 97.5 100 100 100 t00 97.5 97,5 92.5 100 100 100 100 100 100 97.5 95 95 97.5 I00 100 0.40 0.31 0.45 0,58 0.67 0.83 0.67 0.34 0.63 0.52 0.89 0.85 0.54 1.00 0.70 0.82 0.80 0.95 0.65 0.64 C:V CT MSD PMSD Cr C/c) wturCnu(%i Growal CV 0~/.)
7.5 3.6 10.1 13.2 5.3 13.3 10.5 6.5 9.8 9.1 8.3 4,9 2.5 7A4 22.8 16.6 10.t 6.9 R.o 3.3 5.5 7.1 8.6 7.9 8.8 9.1 8.8 8.9 8.9 8.8 8.5 8.0 8.0 9,0 9.5 9,5 9.4 9.3 9.0 0.06 0.07 0.10 0.12 0.07 0,22 0.13 0.08 0,19 0.07 0.13 0.09 0.10 0.17 0.19 0.18 0.21 0.10 0.11 Now:
CV = Coefficient on.rvjrtion for contfrl survival.
On average, the CV for control growth i 9.0.
in Envimv~uncal "lnting Solutions, 1.112 Pinwpholcc chronic toxicity tet;.
tovei CV boutmd determined by JSEPA (IC"'
.lerentile))
-3..*%
Upper CV bound dotennired by USIIPA (9 0n pereendtile)= 20%
MSD = Minimum Sigdaticat Ditfereoea P.MSD -
Percent Minnmnm Significaat Difference PNSI) is a Incasure Of test precision. The PMSD in the mininiuns percent difflcnce b*tweea the contwl and treament that caa be declared statistically significamt in a wbol ct-llueat toxicity test. Ot average. a significant difference occurs for Envir*nmentl t'esfing Solutions, 1.14' Chronic toxicity tests when a toxicant reduces Ptmephaltngrtwit by 1945% Brom the cunroL owerl PMSD bound determined by UJSýPA (I0' pcrceintila) - 9,4%.,
Upper PMSn bound dtcetmined hy (ISF.PA (90QW pm.-tntile) - 35%.
CT' = Central 1'ndaney (mewn Control Growhfl CV Of le-m PMSD) lhe luoer and upper bo*nids were calcuLhted by the USEPA.,hng 205 tew.ss condocted fromn 19 labonturie fur Pinwphaloes grwth in chrunic reference lOxiuitrt lesls.
IISEPA. 2000. Understanding and Acoantling for Mehod Variability in Whole F.Muettl Tox icity Applications Under the National Pollurnt Dischar.*.
uutihimtun lt rogp-A-a..
HPA-i13-R.00-003. US lrnvironrnenrl Prnoetiot Ageny,' Cinuinoiti, 0i.
Organcisns ori.'tauii firm Aquade llioVStreot, m:.
(0/)
for PkMSV (/)
15.7 21.2 22.2 20.0 11.2 26,9 19.3 22.8 30.6 13.8 15.1 10.4 18,6 16.9 26.1 23.5 22.0 22.2 15.5 16.5 1X.4 19.7 19.7 18.0 19.5 19.5 19.9 21.1 20.4 19.9 19.1 19.1 18.9 19.4 19.6 19.8 19.9 19.7 19.5 MRR-08-2302 10:01 P. 036 8288628195
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potas;iu nm Chlorlde Chronic Referzace Toxicant Control Chart for Pimephales prom elas using Moderately Hard Synthetic Water
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S-Central rendency (mean PMSD)
Lower and Upper PMSID Bounds Lower PMSD Bound (10i' percentile) = 9.4%. Upper PIVMSD Bound (90" percentile) = 35%
(Lower and upper PMSD bounds were. determined by USEPA for the method and endpoint.)
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7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC25+/- S A75 SA."7
= 0.38, as determined by USEPA for the method and endpoint)
......... Control Limits (mean IC25 + SA.90, SA.%
A
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S..................................................
Since the coefficient ofvariation for the set of 7-d IC25 values (0.11) is less than the 75?I and 901 percentiles determircd by the USEPA, these percentiles were used in setting the warning and control limits.
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Species: Pimephales promdas PpKCICR Tcst Number:
L3 Dilution prEpatdon inorm,.on:
Comments:
KCI CM1M number C_., 0__,_
Stock preparation:
50 g KCl/L: Dissolve 50 & KC1 in 1-L Deionized watar Dibron prep (rag/L) 300 450 600 750 900 Stc9oii m
12 15 7
DilucMx volmw (mL) 994 991 98t 985 982 Te-vt organi.m Information:.Tet infomta,,:
Organism ag'e:
2'4-k0.9 0 &.t Randomizing template:
__tt__
Date and times organisms 0-'2S.Qz I%'oo Tb K Incubator number:
were born between:
Organism source:
A S 6prTCI" o.-t.,
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olp Transfer bowl information:
pH =
Tempcratae =
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"-"2q,2C Drying time:
Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test Initiation, renewal.
Analyst time time or ermination time o0 2Q.
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Average weight per larvae:
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PpKCICR Tt Number: 13 MIS Control Chemistry:
Paramcter Control Batch:
_Z-__,-_
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pH
.O (s.u.)
at
- o DO (mg/L)
Conductivity Grnihos/cmi)
-\\____
Alkalinity (Mg CaC0WL)
Hardness (mgCaC0 3,L)
Ca_0 3_(_)
Acceptance Criteria for Daily Chenistry:
MAR-08-23082 09:58 Acceptance Criteria Concentration (diluti-m m..t be remade ifthe conductivity fals*
otsido 1ho
_ _ ta*bi rangic)
CONTROL 280 - 360 300 mg KClIL 840 - 890 450 mg KCI/L 1100 - 1180 600 mg KCIIL 1390- 1460 750 mg KCI/L 1660-1710 900 mg KCI/L 1890 - 1950 KCI Stock 67000 - 76000 (25 g KCVL) 82e8628195 P. 02?
ENVIRONMENTAL TESTING SOLUTIONS
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Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphnia dubia using Moderately Hard Synthetic Water Test Tesdat Control nmber Survival Control Mean Reproduction CV Cr MSD PMSD
(.)
(oprine/femae) 100 100 100 100 1oo 100 100 100 100 100 100 100 100 100 100 100 100 100 100 21.8 24.0 25.6 24.8 25.1 28.0 22.1 24.0 25.0 27.9 27.8 25.0 30.9 28.6 24.9 27.0 24.8 27.5 23.1 for Control
(%)
Reprodton CV (%)
11.6 16.0 4.6 9.5 19.2 8.4 10.1 8.3 10.0 8.9 5.8 9.4 2.4 6.6 8.8 6.5 9.1 7.5 10.1 13.8 10.7 10.4 12.2 11.6 11.3 11.0 10.9 10.7 10.2 10.1 9.6 9.3 9.3 9.1 9.1 9.0 9.1 Note:
CV = Coefficient of variation for control reproduction.
On average, the CV for control reproduction is 9.1% in Environmental Testing Solutions, LLC Lower CV bound determined by USEPA ( 10" percentile) = 8.90.
Upper CV bound determined by USEPA (90 t percentile) = 42%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 13.0% from the control.
Lower PMSD bound determined by USEPA (10t percentile) = 1 1/.
Upper PMSD bound determined by USEPA (90+/- percentile) = 37/.
CT = Central Tendancy (mean Control Reproduction CV or mean PMSD)
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH.
2.6 3.5 3.3 4.6 5.0 3.5 3.1 2.4 2.9 3.1 3.5 2.8 2.7 2.7 2.5 3.1 4.8 4.5 2.6
(%)
for PMSD (%)
11.8 14.6 12.9 18.4 19.8 12.4 14.2 10.1 11.8 11.0 12.5 11.2 8.7 9.4 9.9 11.6 19.5 16.3 11.1 13.2 13.1 14.4 15.5 15.0 14.9 14.3 14.0 13.7 13.6 13.4 13.0 12.8 12.6 12.5 12.9 13.1 13.0 02-05-02.xls 1
2 3
4 5
6 7
8 9
10 11 12 13 14 15 16 17 18 19 12-13-00 12-17-00 12-17-00 12-19-00 12-19-00 01-09-01 02-06-01 03-07-01 04-04-01 05-09-01 06-06-01 07-10-01 08-08-01 09-12-01 10-10-01 11-01-01 12-05-01 01-09-02 02-05-02 CTI
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water Test date PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- Central Tendency (mean PMSD)
Lower and Upper PMSD Bounds Lower PMSD Bound (1011 percentile) = 11%, Upper PMSD Bound (909h percentile) = 37%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
50 45 40 35 30 25 20 15 10 5
0
Sodfam Chloride Chronic Reference Toxicant Test (EPAJ600'4-911002 Method 1002.0)
Speciez. Ceiiodaphnia dubla Time nalMCdNAC Teat shta Tesa end ICR Test NumbJer.
1 Diluidon In oration NAQi CHM mnuber-Stodc prearation:
100 gN"c~ (& uahs5" S N~al in'-toom!
800tmpe 1000 1200 1400ý St&vbm(L 12 15 18 21 Dilualvolum (;)
- i1491 142 4.S uR Af Conunb.
Tat In Tmf Organism inLormation:
Organism age-iformadon.
Ob-Rando mizin a
q a2 rsst 'ra tto Date andd timm orgauLms, 02-CfA Incubator nuniber.
Te a O:qee33 were ffbom between:
i f co nbm L Organism source:
YCT batch:
A bowl
'0 Transfer bowl information: I PH= et Of jempcratmeLC)fz Jp Selenaw-um batch:
,41994 1-493 r4I%,wTmrr"rIr Survival and Reproduction Data a
..o-z.
600 me NaCIIL CdNaCICR Test Number:. 4_
Survival and Reroductlon Data Day 1
2 3
4 5
6 7
1 9
10 I
Young produced
.0 0
C 0
_~
L
-~Auk mortlt L..
L..
L.-
c...
L..
Q c
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ýI eYorg produced
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6 Yougproduced 17 C)C oýC 0
0o Aduftortality T
L.T L
7 Young produced
___LA Ilul Adult Mortality f
L.
L Adult~
mooratayity:
7 ~ ~
~
~
~
Ma Offsprodueding/Female:~
j j
Conedcnrtion:fo onrl 800 me NaCI/L Calculations and data reviewed:
Survival and Revroduction Data Replicate number
- Day, 1
2 3
4 5
6 7
8 9
10 1
Young produced
~
Q Adk ortaity L L L
2 Youngproduced 0
0 c0 C
0 [o 0T_
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__L m
=
-__ -It
=,-
=I
=
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Adult mortality I
U..
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e L_.
uI.
E 4
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[_
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T Young produced
-7 1
Adultmortality C
g
=
Ad
=
=
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t 1 (4 12 Total young protduced 125 M
2 Z.
Z5 23
'Iq 7S I z~Zq Final AdultMortaliyUt
~
7
~
7 1
Concentration:
% Mortality:
07 Mean Offspring/Female:
Z2-'7 7% Reduction from Control:
1-18 Calculations and data reviewed:
-ý
CdNaClCR Test Number.
I1 1000 m NaCImL I
a2=tI P',I5£15 rfu5 Dl.aza D ayu m o ta it U
LL Yomun produced 4
FialAdult Mortlt At* Mortity
% Reduction from Control:
13.9 I Calculations and data re'viewed:
1200 mgNaCI/L Survival and Reprodu ction Data
_36 9
10 1
Young produced g'C) p S---
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__L_
__L L
U UL
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=
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~
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U 3
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( -
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-~7 7
6 Young produced
- 0.
M Adultmortaity L
7 Young produced 6
~
Total youngproduced Z 0 i.7,
'zo I
)4 P
FinalAdult Mortality Concentration:
% Mortality:
I 0
Mean Oftring/Female:
- 12.
% Reduction from Control:
W.4.
Calculations and data revi ;7.=
-I--
,-,,., v.vm un*
D*
0
CdNaCICR Te Number. _.
1400mmNaCT/L 1
Young produced
- Adlt mo~rtalt 2
oung produced Adn* mortalit 3
Young produced 4
Young produced Adult mortality 5
Young produced Adult mor~l 6
Young produced Total young produced Mna Adulth Mo Survival andReproduction Data T
numaber SL
- 7.
C) nC L
Concentration:
%Moggaity I
Mean Offsp run/eale:
A I% Reduction from Control:o7&
Calculations and data reviewed:
D-c og~_
Reproduction FY
~I~f HI HI Responset UIi 0<
0 c0 8yPa
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubla Quality Control Verification of Data Entry, Calculations, and Statistical Analyses 1'est number:
CdNaCICR #19 Dunnett's MSD value:
2.571 PMSD:
11.1 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and Ireatmmt that can be decamd statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutioms, LLC cbronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 13.0% from the control.
Lower IIMSD bound determined by USEPA (10b percentile) = 11%.
Upper PMSD bound determined by USEPA (90w percentile) = 37%.
"The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories fir Caelodaphaa trqmodueton in cronic ret~rence toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R.-00R-3. US Favironiaial Protection Agency, Cincinnati, 011.
CdNaCICR Test Number A MO Control Ckemistvy:
[Parameter Control Batch:
o 2-p7- + 2 8 2 -o 1. 2..
DO Conductivity (ghscm)
Alkalinity (mg CaCOJL)
Hardness (mg CaCO3/L) q0 Acceptance Criteria for Daily Chemistry:
I Acceptance Criteria Concentration (dilutio mus be remade if the conductivity falls outside the acceptable range)
CONTROL 280 - 360 600 mg NaCI/L 1390 - 1590 800 mg NaC1/L 1780-1970 1000 mg NaCI/L 2190 - 2370 1200 mng NaCI/L 2550 - 2740 1400 mg NaCI/L 2910-3160 NaCi Stock 110500 - 116300
CdNaaCcR Test Number _IA CONTROL 600 mg NaCI/L 800 mg NaCI/L
,SrocK cpbsn~fv~r/1 II(4&0 ilcyqoc
CdNaCICP. Test Numbe, 11 17,4co WOW
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information, including the possibility of fine and imprisonment for knowing violations.
TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here EPA Form 3320-1 (REV 3199)
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COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference alt attachments here Operations is required once per shift to perform visual inspections.
I ____________________________________________
EPA Form 3320-1 (REV 3199)
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OFFICER O R
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NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
EPA Form 3320-1 (REV 3/99)
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