IR 05000290/2001001
| ML20212E622 | |
| Person / Time | |
|---|---|
| Site: | 05000290, 03031304 |
| Issue date: | 10/09/1997 |
| From: | Prange B NRC OFFICE OF INSPECTION & ENFORCEMENT (IE REGION IV) |
| To: | |
| References | |
| 50-29001-01, 50-29001-1, 572564, NUDOCS 9711040005 | |
| Download: ML20212E622 (20) | |
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, M ATERI Al.S 1,1 CENSE ! Pursuant to the Atomic Energ) Act of 19$4, as amended, the Energy Reorganisation Act of 1974 (Public Law 93 43Bh and Title 10 Code of l
i edera' Ref ulations, Chapter I, Parts 30,31,32,33,34,35,36,39,40, and 70, and in reliance on statements and representations heretofore made ly l by the licensee, a license is hereby tuued authoniing the hcensee lo recch e, acquire, poucu, and transfer by product, source, and special nuclear {{ h( material detignated below; to use such material for the purpose (s) and at the placeN designated below; to deiner persons authorlied to recche it in accordance with the regulations of the apphcable PartN.1hn license shall be deemed to contain the conditions $ t
$l9specined in section Nuclear Regulatory Commission18) nowoforthe Atomic hereaf ter in cIlectEnergy and to anyAtt of 1954, conditions asbelow, specified amended, and in subject to all applicable rules, r l
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l Department of the Interior 50-29001-01 is amended in i M U.S. Geological Survey its entirety to read as follows: p is 1 n p 4 Alaska Biological Science Center 1011 East Tudor Road 4, nxpiration nate October _31._2001 (.l J Anchorage, Alaska 99503 5. Docket or J Reference No. 039-31304 d[ 6 llypniduct, Source, and/or Special Nuclear Material 7, Chemical and/or Physical 8. Masimum Amount that Lkenwe il g. Form May Possess at Any One Time s! gl Under This License y I 4 6 4 A. Phosphorus-32 A. Any A. 5 mil 11 curies
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C. Sul fur-35 C. Any C. 3 millicuries $!
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% A. through C. To be used for molecular genetics laboratory analyses, s'
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} 10. Licensed material shall be used only at the licensee's facilities located at 1011 nj-j East Tudor Road, Anchorage, Alaska, t
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6t f11. Licensed material shall be used by, or under the supervision of, Kim Scribner, ,
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Ph.D., George Sage, John Pearce, Barbara Pierson, or Sandra Telbot. 1 [e l O n \
} 12. The Radiation Safety Officer for this license is John Pearce. <
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- 13. The licensee is authorized to transport licensed material only in accordance with the provisions of 10 CFR Part 71, " Packaging and Transportation of Radioactive f 6 l
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'*"*TW29001-01 E; 6 kl MATERI ALS LICENSE tw" ' MOT'3 h0T'
gll g SUPPLEMENTARY SHEET
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n' i y gl d W N- E n E W 14. The licensee is authorized to hold radiosctive material with a physical half-life of N 4 less than 90 days for decay-in-storage before disposal in ordinary trash provided: N N 5 4 A. Radioactive waste to be disposed of in this manner shall be held for decay a N ' 4 minimum of 10 half-lives. E n F 4 B. Before disposal as ordinary trash, byproduct material shall be surveyed at the N y container surface with the appropriate meter set on its most sensitive scale E y and with no interposed shielding to determine that its radioactivity cannot be a y distinguished from background. All radiation labels shall be removed or a y obliterated, g M N y C. A record of each disposal permitted under this License Condition shall be g g retained for three years. The record must include the date of disposal, the y y date on which the byproduct material was placed in storage, the radionuclides g yl disposed, the survey instrument used, the background dose rate, the dose rate y gl measured at the surface of each waste container, and the name of the individual y l who performed the disposal. gl gkl 15 q . Except as specifically provided otherwise in this license, the licensee shall conduct its program in accordance with the statements, representations, and n y y y , gl procedures containt:d in the documents, including any enclosures, listed below. The g
! , Nuclear Regulatory Commission's regulations shall govern unless the statements, g ,
y representations and procedures in the licensee's application and correspondence are gl
more restrictive than the regulations, g I I A. Letter dated October 11, 1994 k
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1 B. Letter dated October 27, 1994 !I 1 C. Letter dated January 16, 1995 A * E l D. Application dated September 24, 1997 f W E. Letter datert October 9,1997 l W 4 E s W f E FOR THE U.S. NUCLEAR REGULATORY COMMISSION , Il b
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BITWEEN: , - - - License Fee Management Branch. ARM l Pro ram Code: 03620 i Regional LifeNsing Sections b
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{ ACHED Check ho.: l 3. COMMENi$ ba!e' B. LICENSE F[[ MANAGEMENT BRANCH (Check when milestone 03 is entered /_/) 1. Fee Category and Amount: 2. e ce Paid. Application may be processed for:
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f Walnut Creek Field Office g+,,,,,*/ 1450 Maria Lane Suite 300 Walnut Creek, Cahfornia 94596-5368 0CT 0 91997 Department of the Interior U.S. Geological Survey Alaska Biological Science Centor ATTN: John Pearce Radiation Safety Officer 1011 East Tudor Road Anchorage, Alaska 99503 SUBJECT: 1.lCENSE AMENDMENT Please find enclosed License No. 50 20001 01. You should review this license carefully and be sure that you understand all conditions. If you have any questions, you may contact the reviewer who signed your license at (510) 975 0250.
NRC expects licensees to conduct their programs with rueticulous attention to detail and a high standard of compliance. Because of the serious consequences to employees and the public which can result from failure to comply with NRC requirements, you must conduct your program involving radioactive materials in accordance with the conditions of your NRC license, representations made in your license application, and NRC regulations. In-particular, note that you must: 1. Operate in accordance with NRC regulations 10 CFR Part 19, " Notices, Instructions and Repoits to Workers: Inspection and Investigations," 10 CFR Part 20,
"Standardi, for Protection Against Radiation," and other hpplicable regulations.
2. Possess radioactive material only in the quantity and form indicated ?n your license.
3. Use radioactive material only for the purpose (s) indicated in your license.
. 4 ~ Notify NRC in writing of any change In mailing address (no fee required if the location of radioactive material remains the same).
5. Request and obtain written NRC consent before transferring your license or any s right thereunder, either voluntarily or involuntarily, directly or indirectly, through transfer of control of your license to any person or entity. A transfer of control of your license includes not only a total change of ownership, but also a change in the controlling interest in your company whether it is a corporation, partnership, or other entity, in addition, appropriate license amendments must be requested and obtained for any other planned changes in your facility or program that are contrary to your license or contrary to representations made in your license application, as well as supplemental correspondence thereto, which are incorporated into your license. A license fee may be charged for the amendments if you are not in a fee-exempt category.
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Department of the Interior 2 Anchorage, Alaska 6. Maintain in a single document decommissioning records that have been certified for completeness aad accuracy listing all the following items applicable to the license:
* Onsite areas designated or formerly designated as restricted areas as defined in 10 CFR 20.3(a)(14) or 20.1003.
- Onsite areas, other than restricted areas, where radioactive materials in quantitles greeter than amountu listed in Appendix C to 10 CFR 20.1001 20.2401 have been used, possessed, or stored.
- Onsite areas, other than restricted areas, where spills or other unusuel occurrences involving the spread of contamination In and around the facility,-
equipment, or site have occurred that required reporting pursuant to 10 CFR 30.50(b)(1) or (b)(4), including areas where subsequent cleanup procedures have removed the contamination.
- Specific locations and radionuclide contents of previous and current burial
. areas within the site, excluding radioactive material with half lives of 10 days or less, depleted uranium used only for shielding or as penetrators in unused munitions, or sealed sources authorized for use at temporary job sites.
- Location and description of all contaminated equipment involved in licensed operationb that is to remain onsite after license termination.
7. Submit a complete renewal application with proper fee, or termination request at least 30 days before the expiration date on your license. You will receive a reminder notice approximately 90 days before the expiration date. Possession of radioactive material after your license expires is a violation of NRC regulations.
8 Roquest termination of your license if you plan to permanently discontinue activities involving radioactive material.
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Department of the Interior .3 Anchorage, Alaska You will be periodically inspected by NRC. Failure to conduct your program in accordance with NRC regulations, license conditions, and representations made in your license application and supplemental correspondence with NRC will result in enforcement action against you. This could include issuance of a notice of violation; impo6't'on of a civil penalty; or an order suspending, modifying, or revoking your license as a pecified in the
" General Statement of Policy and Procedure for NRC Enforcement Actions" (Enforcement Policy), 60 FR 34381, June 30,1995.
Thank you for your cooperation.
Sincerely, $' M4 Beth A. Prange 6 Sr. Health Physicist (Licensing) Materials Branch Docket: 030 31304 License: 50 29001 01 l Control: 572564 Enclosures: As stated
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Department of the Interior -4-Anchorage, Alaska bec: Docket File WCFO Inspection File LFARB, T 9 E10 State of AK (License Only) DOCUMENT NAME: Gi\beth\572564 To receive copy of document, Ind6cate in bos: *C" = Copy without encbsures *E" * Copy with enclosures "N" = No copy lRIV:MB A' RIV:MB lAJ RIV:MB l/ ' l l-lK Pre 3defgast- BPrange & # JMontgomefy lA
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/97- # 9 /97- f /97 0FFIClAL RLCORD COPY
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d -- United StatcS Department of the Interior U.S. GEOLOGICAL SURVEY BiologicalResources Division
, Alaska Science Center 10ll E. Tudor Road Anchorage. Aluka 99503 '
October 9,1997 Ms. Beth Pran e Sr. Ilealth P cist Nuclear Re atory Ccenmluion, Region IV Walnut Creek Field Office ' 1450 Maria Lane Walnut Creek, CA 94596 5368
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Dear Ms Prange.
As per your our conversation of October 10, I would like to submit additional materials in support of our request for amendment to materials license No. 50-29001-01, which was detailed in my letter of 24 September.
Specifically, in the area of Was.e Management for "P, "P, and "S we will perform the following: A. As per our previous license amendment and renewal requests, we will dispose ofliquid waste widch are in soluble form in electrophoretic buiTers into the municipal sewer system with dilution's oflarge volumes of water as per requirements stated in 10 CFR Pa 120 (Tabh 3 of Appendix B). Records will be kept of quantities ofliquid waste containing licensed material discarded per application, per day. Monthly
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records of waste quantities (pCi) will be maintained.
B. Solid wastes will be segregated by material type an( will be stored separately. Solid waste will be stored in metal containers which are to be housed within large 3/8 inch plexly) ass holding containers.
These containers are to be housed along with outer wall of the dark room adjoining the laboratory. Access to this room i, limited to one entrance from the Molecular E: ology Laboratory. The main laboratory is locked at all times. These long-term holding containers will ba rnonitored weekly and monthly as part of our routine laboratory surveys.
C. Dates of all materials added to the metal containers are list. Each metal container is sealed and the date of closing is listed on the containers. After a period of 10 halflives (from the time of container closing), a random sample of materials is to be removed and radioactivity with be menitored by placing a small portion of each random sample of materials into liquid scintillation vials and counted using a Beckman 7500 counter. Levels of radioactivity will be compared to background standards run at the same time. If levels of radiation are not above background, the samples will be discarded Iflevels exceed background, the samples will be stored for an additional two half-lives and radioactivity levels will be taken agam as outlined above. Solid wastes consist of acrylamide electrophoretic gels which are dried onto Whatman filter paper, This material is the same paper as is used for our monthly swipe tests and this form of monitoring is completely compatible with procedures for beta-emitting materials.
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10/09/97 14:01 C907 Ts0 3636 AK $Cll:NCE: CIR @003/003
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D. Waste centainers will be monitored weekly and monthly as part of the laboratory wide survey as described above.
E. Records of amounts of materials disposed (liquid) and records of total laboratory holdings oflleensed - materials (both waste and unused) will be tallied monthly, b> material type, at the time of the monthly swipe tests and munitoring.
I hope that this text addresses your questions. Thank you for your attention.
Sincerely, ' Kim Scribner Radiation Safety Officer ec Dirk Derksen, Branch Chief, Alaska Dioksical Science Centw Jolm Pearce, Geneticist, Alaska Science Center 57236h
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10/09/97 14:01 C 907 786 3636 AK SCII3CE CIR @001/003
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U.S. Geological Survey Biological itesources Division Alaska Science Center 1011 East Tudor Road Anchorage, Alaska 99503 4199 - - FAX (907) 786-3636 Confinnation (907) 786 3512 YAYAYAYAYAYAYAYAYAYAYAYAYATAYAYAYAYAYAYAYAYAYAY Number of Pages: 2 + Cover Date: 0 8 % O 97-TO: Be% frew K FAX #: __ f*-9F~o39i FROM: Ki m 6 ' ' "4
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TELEPHONE OR VERBAL ColNERSATION-N l TIME RECORD [ /fs.f 5 ' l 00:00amQ ht*%j)b<f INCOMING CALL [ ] OUTGd1NG1ALL [ ] VISIT i lERSON CALLIWGI CTTICE/ADDRESSI Pil0XE WUMB D Dr. Sce:bner ryg g ,g,g,j m (qoy) yg(, _3(,g3 FERSON CALLIDI CTTICE/ADDRE3St U TilofE NtME12: COINERSATION SUBJECT - ' L A dit) Sqt. 2ci,Iqq7/CM Alo. 5 7256 Y
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SUMMARY - l* O d 11te W& SlC OtM &4 f & M Whe4 f is indas Ji- in fue rexLe u e muotG smy is i wA&. s c-M je m c&uy -in - s h"^"y f*7~ ~ n~N.
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14 , , , , , +,8 Walnut Crook Field Office 1450 Maria Lane Walnut Creek, California 94596-5368 0 0 T. 0 3 1997 Department of the Interior U.S. Geological Survey Alaska Science Center ATTN: Kim Scribner Radiation Safety Officer 1011 E. Tudor Road Anchorage, Alaska 99503 SUBJECT: ACKNOWLEDGMENT OF REQUEST FOR LICENSING ACTION REFERENCE: Letter / Application dated September 24,1997 We have completed the administrative review and initial processing of your amendment request.
Please note that the technical review may identify additional ornissions in the submitted information or technicalissues that require additional information.
Amendment actions are liormally processed within 90 days, unless the technical review identifies:
* Major technical deficiencies *
Policy issues that require input and coordination with other NRC Regional offices.
Agreement State offices, or NRC's Office of Nuclear Materials and Safeguards Any correspondence about this application should reference the Control number listed below.
Sincerely, O.
Beth A. Prange Senior Health Physicist (Licensing) Materials Branch Docket No. 030 31304 License No. 50 29001-01 Control No. 572564
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Department of the interior 2-i bec: , Docket File , , i i
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G:\572564.aki To receive a copy of this document, indicate in the box "C" Copy without attachment / enclosure "E" Copy with attachment / enclosure "N" No Copy OFFICE RIV:WCFQ:MB N NAME J. Garcl[lj t* B. Prange M DATE [d/h97[/ /v /3/97 i
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U.S. GliOLOGICAl. SURVI y 9 007 ', b /21 M tholorial Rewurses Dniuon nnennnam Alaska Sciense Cetuer 1011 it Tudor Road l ' Anthotare. Alaska W503 September 24,1997 his. Ileth Prange Sr. llealth Physicist Nuclear Regulatory Commission, Region IV Walnut Creek Field Ollice 1450 h1 aria Larie Walnut Creek, CA 94596-5368
Dear his Prange:
Please find enclosed materials in support of our application for amendment of materials license (No 50 29001-01). Please be advised that I will be leaving the Alaska Science Center to take a new position beginning December 1 of this year. I will thus be stepping down as the Radiation Safety Ollicer for this laboratory. Detailed in this amendment are 1) the background and experience of John Pearce whom I wish to nominate as my successor, and 2) the background of several new employees (Ilarbara Pierson and Sandra Talbot) which I would request be added as unsupenised materials users. This amendment request is otherwise essentially unchanged from the amendment we were granted at the end of 1996.
I strongly recommend that John Pearce would be a suitable choice for my successor. hir. Pearce has coordinated all ourlabort.mry monitoring and dosimetry duties for the past year. John has been famially trained and has served in a similar capacity in a teaching laboratory m a universit has been associated with all activities of this laboratory for over 3 years. lie is thorough,y conscientious, sett and diligent. I give John my highest recommendation As an additional detail to consider, please note that our Center's nan,e has been changed from the Alaska Science Center to the Alaska Ilicological Science Center Our Center remains a part of the Iliological Resource Division of the Geological Survey.
, I ho >e that you find all materials to be in order and that our application can be processed as soon as pos'ble. Thank you in advance fcr your attention,
Sincerely,
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Kim Scribner Radiation Safety Oflicer cc Dirk Derksen, Branch Chief, Alaska Iliological Science Center John Pearce, Geneticist, Alaska Science Center A i
e NRC apaiM 313 , ilt t Fi U g g;UCLEA3 8.85pl&TORv Coastes10ss APPhovt0 0Y past
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A New uCiNn Alaska Biological Scien~e Center
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e.AMikoMINTio uCINu NUM6th m - s u m u v H 1- . Biological Resource Division, U.S. Goologica L
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C. McNtwAL os uC NatNUM9th Survey 1011 E. Tudor Bd. , Anchorage, AK 99503 3. ADOAt681681 WMtRt LICEN84 D MATIR4AL wlLL St V640 OR pol $1464D Alaska Biological Science Center-Molecularr.cology laboratory 1011 E. Tudor Bd.
Anchorage, AK 99503 A .eAMs 0. PERSON to St Coni AcilD ABOU, TM APPuCAuoN lnuPHONENUM84R Kim Scribner 907-786-3683 SUCMIT ITEMe l THROUGH 11 ON eh e 11* f APER. THE TYPt AND SCOPE Of INPORMAf t0N to t t PROVIDio ll DESCRittD IN IME LICENlt APPLICAT104 0Ut05.
6. RADtOACTIVE MATERIAL e tiemont er,e maan numter, S. shemwal enwee phreecet torm, ead e mesenvm emovat a PURPO$t tli f 0h WHICM LICEN5tD MATERI AL wILL DE U5tO.
which moti to poseseena et one one tune IhDA q gguA 8 a b EksLt Ci 90R R ADIAttON sap TTY PR00 RAM ANO TMtim 8 TRAININO FOR INDIV10 VAL 8 w0'tKtN0 lN OR tRt0 VENTING Rt81RICTED ARI A8 9 F ACILitl%S AND t0VIPMtNT. 10. MADI Af TON Saf tTV PROGRAM 13 L:044444 tit $ (See 80 C#4 t/p eas $ertsen 870 Jet 11, Wed. Sit MANAGEMENT. UNT 13 Cl ATIFICATION tesyst teremPeelvr'Dy assWseafJ TMt APPLICANT UNotRSMNDS TMAf 4L ST AitMENTS AND RtPRtStN1 AllONS MADS IN TM'l APPLICATION ARE glNDINO VPON TM4 APPLIC ANT.
TMt APPLICANT AND ANY 0*FICIAL t rtCUTING TMit CE Riffis ATION ON tt MALP OF THP APPLICAN1.8eAMID IN ITEM 2. CERTIF Y THAT TMit APPLICATION 18 Pit 4 PARED IN CONFORMITY wiTM TITLE 10. CODE 0* 8 E0 TRAL RIOUL AT60N&.PART8 30,32,33. 34, al, AND 40 AND 1H AT ALL INFOhWAf t0N CONT AINED MERLIN, 48 TRVt AND CORRICT TO THE StST OF THEIR eLN0wLt005 AND StutP.
WAntetNO 18 U 8 C $tCTION 1001 ACT Of JUNt 76. tDet 62 ST AT. ist MetiS IT A CRiWie AL Ott f eelt TO M AKt A wtLLFULLY $ AL$t $T ATIMENT OR RtPntstNT Att0N TO ANY DEPARTMENT * A068eCY Of TMt UNITED 81 Atts Al TO ANY MATTER witMIN ITS JUR15Dicit0N SIGNat uRE-Cim fit vik 09 P ectr TYP6 0/PRINilD NAMt TIT L E DAT4
/b $ Mm Scribner Besearch Geneticist
_ 9-24-97 FOR NRC U$a ONLY TVsEOF$45 ~ t ti LOG ~YttCAft00RY COMYENTS APPRovtD B4 AMOUNTRICilytD CMtCK NUM9tR DAtt er
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APPLICATION FOR MATERIAL LICENSE 5. RADIOACTIVE MATERIAL a. element mass #
(1). Phosphorous 32 (2). Phosphorous 33 (3). S ulf u r.3 5 b chemical form (1), a DCTP (alpha deoxycytidine triphosphate) or u dATP (alpha deoxyadenosine triphophate) or y-ATP (gamma adenosine triphosphate, or y-CTP (gamma cytidine triphosphate) (2), y-ATP (gamma adenosine triphosphale) (3), a DCTP (alpha deoxycytosine triphosphate) or a dATP (alpha deoxyadenosine triphosphate)
c. maximum amount (1). 5 millicurles (2). 1 millicurie (3). 3 millicuries 6. PURPOSE FOR WHICH LICENSED MATERIAL WILL BE USED.
Licensed materials will be used for molecular genetic analyses. DNA will be radioactively labeled and used in the following manner: a. as a probe for DNA DNA hybridization to southern blots (32p), b. In sequencing reactions which will be run on polyacrylamide gels (35 S ,33p,32p), c. In polymerase chain reactions (PCR) which will be run on polyacrylamide gets (32p, 33p), d. end labeling DNA restriction fragments (3SS) and running products on agarose gels.
7. INDIVIDUAL (S) RESPONSIBLE FOR RADIATION SAFETY PROGRAM AND THEIR TRAINING AND EXPERIENCE.
All personnel are fully trained in the handling, storage, and disposal of radioactive materials. Overall supervision of the laboratory pertaining to the use of materials and training , of personnel will be the responsibility of the Radiation Safety Officer, fiagation Safetv officer: John Pearce, B.S. degree in Biology, Lewis and Clark College and M.S.
dagree, University of Idaho. John Pearce has nearly 3 years of laboratory experience with the use of Cl quantitles of radioactive materials (principally "P and within the last year "S) In molecular genetics research. Mr. Pearce has received formal training instruction by the Environmental Health and Safety Office of the University of Idaho and by the former Radiation Safety Officer at the Alaska Biological Science Center. Training at both facilities involved instruction in the handling, storage, dispoal and monitoring of licensed materials as well as dosimetery calculations inva'ving long-term storage and disposal and intentory record keeping.
Mr. Pearce has been in charge of radiation safety and monitoring in a laboratory at the University of Idaho for six months during his M.S. degree. For the past year Mr. Pearce has been in charge of monitoring activilles at the Alaska Biological Science Center and has coordinated the laboratory's monthly inventory, dosimetry, and disposal reporting, Mr Pearce
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has been involved in all aspects of materials ordering, receiving, and reporting activities, and has extensive experience in the use of licensed materials in all molecular genetics applications currently employed at the Alaska Biological Sciences Center.
APPLICATION FOR MATERIAL LICENSE , Research Technician: Mr. George Sage. B.S. and M.S. degrees in Zoology from the University of Montana. Four years experience with molecular genetic applications of the use of Cl quantitles of 32P. Training includes formal instruction by the laboratory Radiation Safety Officers at the U.S. Geological Survey Blological Resources Division, Alaska Biological Science Center and Department of Zoology at the University of Montana. Training at both facilities involved instruction in the handling, storage, disposal, and monitoring of licensed materials as well as dosimetry calculations involving long term storage and disposal and inventory rocord keeping.
Mr. Sage has further panicipated in a 3 hr training courses at the University of Montane pertaining to safety issues involving tho handling, monitoring, storage, and disposal of licensed materlats.
,Research Technician: Ms. Barbara Pierson, B.S. Degree in Animal Science, University of
=llinois. Ms. Pierson has 1 year of experience in the use of Cl quantitles of "S and "P for molecular genetics research. She has received formalin laboratory radiation safety Instruction at the University of Illinois and at the Alaska Biological Se' .nce Center. Tre:ning at the Alaska Biological Science Center involved instruction in the handling, storage, disposal, and monitoring of licensed materials as well as dosimetry calculations involving long-term storage and disposal and inventory rocord keeping.
Research Scientist. Sandra Talbot, B.S. University of Alaska, M.S. Brigham Young University, PhD, University of Alaska. Ms. Talbot has extensive background and training in the handling, disposal, and storage of radioactive materials in molecular genetics research. Ms. Talbot has used Cl quantitles of "P and "S for a period of 5 years. She has received formal training through a radiation safety course at the University of Alaska and has been formally trained by the former Radiation Safety Officer at the Alaska Biological Science Center.
8. TRAINING FOR INDIVIDUALS WORKING IN OR FREQUENTING RESTRICTED AREAS, All full time amployees presently working in the laboratory have received formal training in the use, containment, monitoring, and disposal of radioactive materials. For all other potential users of licensed materials and workers using laboratory facilities, a thorough training course will be required before workers can participate in lab activities. Workers who are in the laboratory, but not working directly with licensed materials, will t e informed of the hazards of the isotopes being used. Locations of material used will be idantified. Locations of all fEC mandated materials covering matenals use and exposure will be identified, and workers will bo required to read all necessary materials. Personnel who may be working with licensed material will be required to participate in an extensive orientation course provided by the Laboratory Radiation Safety Officer. This course will cover all fRC and Labcratory policies for the safe , handling, monitoring, containment, 9nd disposal of radioactive materials, j i 9. FACILITIES AND EQtilPMENT The laboratory design has been enclosed (Attarthment A). The facilities include a main lab, support lab, and darkroom / storage area. The area designated as a materia's use area is indicated by a dashed line.
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, i i The laboratory is equipped fot molecular genetic analyses and includes DNA thermal cyclers,
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Die sequencing apparatus and power supplies, horizontal and vertical agarose gel apparatus, incubators, a speed vacuum and gel drier, hybridization oven, freezers, etc.
The majority of work with licensed materials will be conducted in the fume hood (#5).
- Plexiglass shielding and confalners for stored materials and waste will be used in this unit.
Isotopes in current use will be stored in a plexiglass container located in the support lab . I freezer (#2). Long term storage of liquid and solid waste will be in plexiglass containers in location #6. All materials, bench space, and equipment where radioactivity la to be used will be properly labeled. X ray cassettes and sealed containers will be used for filters and sequencing gels. A designated disposal sink (#3) is shielded and will be used for washing equipment and the , disposal of liquid m,'erials.
APPLICATION FOR MATERIAL LICENSE 10. RADIATION SAFETY PROGRAM.
Warning signs and labels will be placed on the laboratory entrances and on bench surfaces and equipment used for radioactive work. Employee safety notices, documentation of hazardous materials, and the location of NRC Information on our site license will be posted.
No eating, orinking, smoking, or storage of food or drink will be allowed in any portion of the laboratory.
Personal protective equipment to include lab coats, safety glasses, and gloves will be worn at all times when using licensed materials. Personnel monitoring will be through film badges (finger ring devices) to be worn at all times when using licensed materials.
New personnel will take an extensive orientation course to be administered by the Laboratory Radiation Safety Officer, This course will cover all aspects of the handling, storgge, disposal, monitoring, and containment of radioactive materials, as well as standard operating procedures for experiment specific use (see above).
Dosimetry monitoring of laboratory personnel will be conducted using ring finger badges.
Badges will be changed monthly. Dosimetry services will be provided by Landauer, Inc.
Two band held survey meters will be used in the laboratory. Servicing and calibration will be done by Ludlum Measurements, Inc. every 12 months.
Monitoring will be conducted as follows:
(1), in the area of use each time licensed materials are used, Us'is of licensed materials will sign a log indicating type of material used, amount used, and wik monitor the use area before and after materials have been used.
(2). weekly foi all designated areas.
Weekly lab wide surveys will be conducted using the hand held survey meters to monitor all bench space, equipment, and storage areas.
(3). monthly in the form of swipe tests.
Swipe tests will be conducted monthly at each of a series of designated locations.
Monitoring will be done by laboratory personnel and measurements obtained using a Beckman LS7000 scintillation counter at the University of Alaska Anchorage.
11. WASTE MANAGEMFNT \ , T
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Wastes will be disposed according to guldelines established in 10 CFR Part 20. Liquid wastes generated will be in soluble form from DNA DNA hybridization filter washes and agarose end sequencing gel buffers. Liquid wastes, not to exceed concentratiens listed in Table Ill of Appendix B for 10 CFR Part 20, will be flushed down the designated sink with a great excess of water. Solid wastes will be stored by specific nucleotide in individual con'ainers behind plexiglass in area #6 of the laboratory diagram. Solid wastes will be stored for a period of 10-half lives, monitored using a scintillation counter, and if below acceptable levels, discarded, i
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