ML092750409

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Reservoir Ichthyiplankton Studies, September 2008 Through August 2009
ML092750409
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Site: Monticello, Summer  Xcel Energy icon.png
Issue date: 09/30/2009
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Normandeau Associates
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Office of New Reactors
References
R-21448.001
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MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES, SEPTEMBER 2008 THROUGH AUGUST 2009 SEPTEMBER 2009 MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES, SEPTEMBER 2008 THROUGH AUGUST 2009 Prepared for Tetra Tech NUS 900 Trail Ridge Road Aiken, SC 29803 Prepared by NORMANDEAU ASSOCIATES, INC.25 Nashua Road Bedford, NH 03110 R-21448.001 September 2009 MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008-- AUGUST 2009 Table of Contents Page

1.0 INTRODUCTION

..........................................................................................................

1 2.0 METHODS AND MATERIALS

.......................................................................................

1 3.0 RE SU LT S .................................................................................................................................

2 4.0 D ISC U SSIO N ..........................................................................................................................

3 5.0 LITERATURE CITED .......................................................................................................

4 APPENDIX 21448.001 Summer Station Ichthyo Report.doc 9/28/09 ii Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008 -AUGUST 2009 List of Figures Page Figure 2-1. Ichthyoplankton sampling locations in Monticello Reservoir September 2008 through A ugust 2009 .......................................................................................................

6 Figure 2-2. Sampling gear used in the Monticello Reservoir ichthyoplankton studies ....................

7 Figure 2-3. Preservation of samples from the Monticello Reservoir ichthyoplankton studies ...... 8 Figure 2-4. Collection of water quality data during the Monticello Reservoir ichthyoplankton stu d ie s ....................................................................................................................................

8 Figure 2-5. Analysis of ichthyoplankton samples collected during the Monticello Reservoir ichthyoplankton studies ...................................................................................................

9 Figure 2-6. Young-of-the-year white perch collected during the Monticello Reservoir ichthyoplankton studies ...................................................................................................

10 Figure 3-1. Monthly density (no./100 M 3) of ichthyoplankton in Monticello Reservoir, Septem ber 2008 through A ugust 2009 ...............................................................................

11 List of Tables Page Table 2-1. Sample design for the V.C. Summer Nuclear Station Ichthyoplankton Studies ..........

12 Table 3-1. Monthly density (no./100 in 3) of ichthyoplankton, young-of-the-year and yearling fish collected in Monticello Reservoir September 2008 through August 2 0 0 9 .....................................................................................................................................

13 Table 3-2. Average density (no./100 M 3) and percent composition of ichthyoplankton collected in Monticello Reservoir September 2008 through August 2009 ..................

15 Table 3-3. Annual entrainment estimates for V.C. Summer Nuclear Station Units 2 and 3 under typical and maximum water withdrawal rates ..................................................

16 21448.001 Summer Station Ichthvo Reeort.doc 9/28/09 iii Normandeau Associates.

Inc...................................

j .... p ..............

.... j MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009

1.0 INTRODUCTION

Ichthyoplankton (fish eggs and larvae) samples were collected in Monticello Reservoir to provide estimates of entrainment of ichthyoplankton at the proposed V.C. Summer Nuclear Station Units 2 and 3 Raw Water Intake Structure.

Although both units would use closed-cycle cooling (mechanical draft cooling towers), evaporative losses would require withdrawal of makeup water from Monticello Reservoir.

Makeup water withdrawal rates would be 36,214 gpm (137.08 m 3/min) under typical operating conditions and 58,800 gpm (222.58 m 3/min) under maximum operating conditions.

The purpose of this study was to collect ichthyoplankton data from Monticello Reservoir to estimate the number of ichthyoplankton potentially lost due to withdrawal of make-up water.2.0 METHODS AND MATERIALS Ichthyoplankton samples were collected at two parallel transects in the vicinity of the proposed intake (Figure 2-1) using 0.5 m-diameter, 0.300 mm-mesh bongo nets fitted with calibrated flowmeters (Figure 2-2). Tow length was about 250 m and each side of the bongo net filtered at least 50 M 3.The two sides of the bongo net were composited in the field, resulting in samples that ranged in volume from 105 to 191 M 3.Samples were preserved in the field with 5% formalin (Figure 2-3). Sampling started in September 2008 and continued for 12 consecutive months. Samples were collected once per month from September 2008 through February 2009, twice per month from March through July 2009, and once in August 2009 for a total of 17 sampling events (Table 2-1). This sampling regime was designed to reflect the reproductive behavior of resident fish species, all of which spawn in the spring and early summer.During each sampling event, samples were collected at the surface and mid depth during both the day and night for a total of 68 samples collected during the study.Additional information collected during sampling included surface, mid-depth, and near bottom water temperature

(°C) and dissolved oxygen concentration (mg/1), air temperature

(°C), and weather conditions

(% cloud cover, precipitation, wind speed and direction, and wave height) (Figure 2-4).Samples were forwarded to Normandeau Associates' biological laboratory where ichthyoplankton were identified to the lowest practical taxon, usually species, and enumerated as to lifestage:

egg, yolk-sac larvae (YSL), post yolk-sac larvae (PYSL), young of the year (YOY), and yearling (age 1) (Figure 2-5). No eggs were found in any samples and subsampling was not required for any species or lifestage.

Data were standardized to no./100 m 3 of water sampled. Since the proposed Raw Water Intake Structure will withdraw from all depths and operate 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> per day, monthly mean ichthyoplankton densities and 95% confidence intervals were calculated across depth and diel samples collected each month to provide water column and time-integrated estimate of densities.

To estimate potential entrainment of ichthyoplankton on a monthly basis, monthly mean densities and their upper and lower 95% confidence limits (no./100 in 3) were multiplied by the estimated monthly water withdrawal rates (typical:

137.08 m 3/min; maximum: 222.58 m 3/min). These monthly estimates were then summed to provide annual estimates of entrainment under the two proposed water withdrawal rates. Although YOY and yearling fish (Figure 2-6) were sometimes captured in the nets, which were towed at about 3 ft/sec, it is unlikely that they would be entrained at the proposed Raw Water Intake which would have a through-screen velocity of 0.5 ft/sec. These fish are included in data tables where the entire catch is presented but 21448.001 Summer Station Ichthyo Report.doc 9/28/09 I Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 excluded from analyses of ichthyoplankton density and percent composition because they are not part of the ichthyoplankton community.

Furthermore, YOY and yearling fish in most cases would be too large to be entrained through the proposed screens which will have 3/8-inch perforations.

3.0 RESULTS Although ichthyoplankton were sampled from September 2008 through August 2009, fish larvae were found in samples only from March through August (Table 3-1, Appendix Table 1). No fish eggs were collected in any samples, presumably because all resident species have demersal and adhesive eggs that would not normally be found in the water column. Prior to the first appearance of fish larvae in samples, a YOY catfish was captured in September 2008 and a YOY threadfin shad was captured in December 2008. Fish larvae first appeared in March 2009 samples and peaked in density in May at 125.15 organisms/100 m 3 (Figure 3-1). After this peak, density decreased rapidly to 0.41/100 m 3 in August (Figure 3-1).In March, threadfin shad YSL were numerically dominant (21.76/100 in 3), but small numbers of white perch, true percids (yellow perch and darters), and black crappie larvae were present. In April, substantial numbers of both YSL (17.97/100 m 3) and PYSL (14.72/100 M 3) threadfin shad were collected as the YSL larvae from March matured into PYSL. White perch YSL (15.55/100 M 3) and PYSL (7.41/100 M 3) increased in abundance in April. By May, most threadfin shad larvae had developed into PYSL (98.27/100 M 3) but some YSL were still present (17.91/100 M 3). Densities of YSL (0.46/100 in 3)and PYSL (3.46/100 in 3) white perch decreased from their monthly high in April. In June, PYSL clupeids (28.00/100 M 3) were dominant and total density of fish larvae was markedly lower, approximately 25% of the total density in May. PYSL Lepomis sp. (1.42/100 M 3) and PYSL clupeids (1.35/100 M 3) were predominant in July. By August, total density of fish larvae was very low and only yearling blue catfish (0.21/100 M 3) and PYSL Lepomis sp. (0.2 1/100 M 3) and clupeids (0.20/100 M 3) were present.For the 12-month sampling period, PYSL and YSL threadfin shad had the highest density and together with undetermined-stage threadfin shad composed approximately 71% of the total catch (Table 3-2). White perch larvae were next in abundance with YSL and PYSL lifestages making up approximately 13% of the catch. The family Clupeidae, which was most likely a combination of threadfin shad and gizzard shad larvae, made up approximately 12% of the catch. All other species and lifestages combined made up less than 4% of the catch.Estimates of potential entrainment were developed by multiplying ichthyoplankton densities by estimated monthly water withdrawal rates (typical:

137.08 m 3/min; maximum: 222.58 m 3/min). An estimated 15.3 million larvae would be entrained annually under the typical water withdrawal rate and 24.9 million under the maximum water withdrawal rate (Table 3-3, Appendix Table 2), based on 2008-2009 densities.

The monthly pattern and species composition of entrainment estimates mirrored the monthly patterns in larval density (Figure 3-1) because the estimates are the product of larval densities and a constant (the two water withdrawal rates). Entrainment is expected to be very low in the fall and winter (September through February), begin to increase in March, reach a peak in May, and decrease rapidly through August (Appendix Table 2).Entrainment estimates were highest for threadfin shad larvae, which made up 71% of all larvae potentially entrained, under typical (10.9 million larvae) and maximum (17.6 million larvae) water withdrawal rate scenarios (Table 3-3). White perch entrainment estimates ranked second under typical 21448.001 Summer Station Ichthyo Report.doc 9/28/09 2 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008 -AUGUST 2009 (1.9 million larvae) and maximum (3.1 million larvae) water withdrawal rates. White perch larvae made up 13% of the total entrainment estimate.

Clupeids were the third most abundant species potentially entrained under typical (1.8 million) and maximum water withdrawal rates (2.9 million).

Clupeids were 12% of the total entrainment estimate.Appendix Table 3 presents the water quality information collected during the ichthyoplankton study. As expected, temperature and DO levels had an inverse relationship due to the higher saturation levels of DO in colder water. DO levels were consistently highest in January (11. 1-11.3 mg/1) when water temperatures were among the lowest (10.6-12.2

'C). Conversely, the lowest DO levels (3.3-3.9 mg/1) occurred in August and September when water temperatures were greater than 27.0 'C. There was a general trend of decreasing DO levels with increasing depth. Appendix Table 4 presents the environmental conditions during sampling.

All environmental parameters were within ranges that allowed collection of valid ichthyoplankton samples.4.0 DiSCUSSION Threadfin shad and gizzard shad, members of the family Clupeidae, together made up approximately 73% of all the larvae collected in Monticello Reservoir and the same percentage of the entrainment estimate.

The category Clupeidae probably included only threadfin shad and gizzard shad, as no other clupeids are known to occur in Monticello Reservoir.

Blueback herring have been reported from the Broad River, but these alleged occurrences have been described as "perplexing" (Rohde et al. 2009)and "questionable" (Bettinger et al. 2003) by authorities on South Carolina's fishes. Assuming all the clupeid larvae were threadfin and gizzard shad, these two species made up 85% of all the larvae collected and the entrainment estimate.

Both threadfin and gizzard shad are important prey species in southern reservoirs and often contribute the most to fish biomass (Allen and DeVries 1993). Threadfin shad normally reach sexual maturity as yearlings.

Threadfin shad may spawn once or several times over a spawning season, with fecundity (number of eggs per ovary) ranging from 800 to 21,000, depending on age and size of the female (Johnson 1971; Jenkins and Burkhead 1994). Gizzard shad typically mature at age 2 or 3. Each female produces 22,400 to 543,900 eggs (Jenkins and Burkhead 1994), depending on age and size, with some studies showing declining fecundity in older fish (Williamson and Nelson 1985).Both species spawn in the spring when water temperature is greater than 15 'C, but gizzard shad usually spawn slightly earlier (Netsch et al. 1971). Both species use shallow water habitats for spawning, but threadfin shad tend to spawn near the surface while gizzard shad tend to spawn near the bottom (Shelton et al. 1982). Larval threadfin shad tend to move offshore and larval gizzard shad remain evenly distributed within 50 m of shore (Allen and DeVries 1993). After the larval stage both species are important prey species, but gizzard shad quickly grow to be too large to be consumed by most predators (Noble 1981). Therefore, threadfin shad is more valuable as a prey species.White perch were the next most abundant larvae, accounting for 13% of both the larvae collected and the entrainment estimate.

The white perch is a semi-anadromous species, native to Atlantic Coast estuaries, but has been established in many freshwater lakes and impoundments.

They have apparently been introduced to the Santee River watershed and Monticello Reservoir (Rohde et al. 2009; Warren et al.2000). Female white perch mature at age 3 or 4, and may produce from 20,000 to 150,000 eggs (Jenkins and Burkhead 1994). White perch spawn in the spring over sand and gravel substrates and there is evidence that reservoir populations may migrate to shallower water to spawn (Rohde et al. 2009). White 21448.001 Summer Station Ichthyo Report.doc 9/28/09 3 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008 -AUGUST 2009 perch are important recreational fish through much of their native range; however, when stocked in reservoirs they can become stunted and compete with native fish.Dames and Moore (1985) conducted an ichthyoplankton study in Monticello Reservoir in 1983-1984 as part of the original 316(b) demonstration for V.C. Summer Station. In their study, total larval density was 53.9 larvae/100 m 3 at the surface and 11.8 larvae/100 m 3 at mid-depth at a station that was a short distance from our sampling stations.

At both depths combined, gizzard shad (61.9/100 in 3; 94%)were the dominant larval species and white bass ranked second in abundance (3.1/100 M 3; 5%). These densities are higher than those observed in the current study and threadfin shad, the dominant species in this study, was not observed.Reservoirs in the southeastern United States are generally most productive in the first several years after impoundment, as topsoils containing agricultural fertilizers and decaying organic matter are inundated by the rising waters of the reservoir.

After this initial burst of productivity, these reservoirs tend to show a decline as nutrients are depleted and sedimentation effectively seals the reservoir bottom.With increasing age, reservoirs depend more and more on nutrients brought in by inflowing riverwater, levels of which may vary seasonally and annually depending on weather and the degree of watershed development.

It's unclear if the apparent decline in Monticello Reservoir ichthyoplankton densities observed between the 1983-1984 study (Dames and Moore 1985) and the current study is related to lower reservoir productivity or is a reflection of normal biological variability.

Spawning success and survival of larval threadfin and gizzard shad (which dominated larval fish collections) are unpredictable and influenced by an array of density-dependent (intra-specific and inter-specific competition, predation) and density-independent (weather, which determines temperatures and rainfall patterns) factors that are outside of the scope of this study.Based on the current study, an estimated 15.3 million fish larvae would be entrained under typical operating conditions with 95% confidence limits of 2.2 million and 30.8 million. These confidence limits vary by a factor of about 15 and reflect seasonal variability.

Annual variability is demonstrated by the differing results between the current study and the Dames and Moore (1985) study which reported ichthyoplankton densities an order of magnitude greater. Furthermore, threadfin shad, the dominant larval species in the current study was not reported by Dames and Moore (1985). The ichthyoplankton community of Monticello reservoir is dominated by very few species and therefore is potentially subject to high variability.

A complete or partial failure to spawn by a dominant species such as either threadfin shad or white perch due to natural causes would result in low ichthyoplankton density and a similar reduction in potential entrainment.

Considering the dynamic nature of the fish community in Monticello Reservoir, especially the possibility of further introductions, of non-native fish such as white perch, large variations in ichthyoplankton density and potential entrainment is to be expected.5.0 LITERATURE CITED Allen, M.S. and D.R. DeVries. 1993. Spatial and temporal heterogeneity of larval shad in a large impoundment.

Trans. Amer. Fish. Soc. 122:12070-1079.

Bettinger, J., J. Crane, and J. Bulak. 2003. Broad River Aquatic Resources Inventory Completion Report. Broad River comprehensive Entrainment Mitigation and Fisheries Resource Enhancement Program. South Carolina Department of Natural Resources.

21448.001 Summer Station Ichthyo Report.doc 9/28/09 4 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Dames & Moore. 1985. 316(b) Demonstration for the Virgil C. Summer Nuclear Station for the South Carolina Department of Health and Environmental Control and the Nuclear Regulatory Commission.

Job. No. 5182 108-09.Johnson, J.E. 1971. Maturity and fecundity of threadfin shad, Dorosoma petenense, in central Arizona reservoirs.

Trans. Amer. Fish. Soc. 100 (74-85).Jenkins, R.E. and N. M. Burkhead.

1994. Freshwater Fishes of Virginia.

American Fisheries Society, Bethesda, MD.Netsch, N.G., G.M. Kensh, Jr,. A. Houser, and R.V. Kilambi. 1971. Distribution of young gizzard shad in Beaver Reservoir.

Amer. Fish. Soc. Special Pub. 8:95-105.Noble, R.L. 1981. Management of forage fishes in impoundments of the southern United States. Trans.Amer. Fish. Soc. 110:738-750.

Rohde, F.C., R.G.Armdt, J.W.Foltz, J.M. Quattro. 2009. Freshwater Fishes of South Carolina.

The University of South Carolina Press.Shelton, W.L., C.D. Riggs and L.G. Hill. 1982. Comparative reproductive biology of the threadfin and gizzard shad in Lake Texoma, Oklahoma-Texas.

Pages 47-51 in C.F. Bryan, J.V. Conner, and F.M. Truesdale, editors. The fifth annual larval fish conference.

Louisiana Cooperative Fisheries Research Unit and the School of Forestry and Wildlife Management.

Louisiana State University.

Baton Rouge.Warren, M.L. and 11 others. 2000. Diversity, distribution, and conservation status of native freshwater fishes of the southern United States. Fisheries 25(10):7-31.

Williamson, K.L. and P.C. Nelson. 1985. Habitat Suitability Index Models and Instream Flow Suitability Curves: Gizzard shad. Habitat Evaluation Procedures Group, U.S. Fish and Wildlife Service, Fort Collins, CO. Biological Report 82(10.112).

21448.001 Summer Station Ichthyo Report.doc 9/28/09 5 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Figure 2-1. Ichthyoplankton sampling locations in Monticello Reservoir September 2008 through August 2009.21448.001 Summer Station Ichthyo Report.doc 9/28/09 6 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Figure 2-2. Sampling gear used in the Monticello Reservoir ichthyoplankton studies.21448.001 Summer Station Ichthyo Report.doc 9/28/09 7 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Figure 2-3. Preservation of samples from the Monticello Reservoir ichthyoplankton studies.---Figure 2-4. Collection of water quality data during the Monticello Reservoir ichthyoplankton studies.21448.001 Summer Station Ichthyo Report.doc 9/28/09 8 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008 -AUGUST 2009 Figure 2-5. Analysis of ichthyoplankton samples collected during the Monticello Reservoir ichthyoplankton studies.21448.001 Summer Station Ichthyo Report.doc 9/28/09 9 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Figure 2-6. Young-of-the-year white perch collected during the Monticello Reservoir ichthyoplankton studies.21448.001 Summer Station Ichthyo Report.doc 9/28/09 10 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 140 120 -U:5 100 _80o*-4o o 60 40 40 20 20 Sep Oct Nov Dec Jan Feb Mar Apr May Jun Jul Aug Month Figure 3-1. Monthly density (no./100 in 3) of ichthyoplankton in Monticello Reservoir, September 2008 through August 2009.21448.001 Summer Station Ichthyo Report.doc 9/28/09 I1I Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008-- AUGUST 2009 Table 2-1. Sample design for the V.C. Summer Nuclear Station lchthyoplankton Studies.Sampling Diel Month Events Depths Periods Samples September 1 2 2 4 October 1 2 2 4 November 1 2 2 4 December 1 2 2 4 January 1 2 2 4 February 1 2 2 4 March 2 2 2 8 April 2 2 2 8 May 2 2 2 8 June 2 2 2 8 July 2 2 2 8 August 1 2 2 4 Total 17 2 2 68 21448.001 Summer Station Ichthyo Report.doc 9/28/09 12 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Table 3-1. Monthly density (no./100 m 3) of ichthyoplankton, young-of-the-year and yearling fish collected in Monticello Reservoir September 2008 through August 2009.Species_ Density LCL UCLV Year Month Species Lifestage a (no./100 m3 (no./100 I 3) (no./100 in 3)2008 Sep Channel Catfish YOY 0.19 0.00 0.81 Oct None captured Nov None captured Dec Threadfin Shad YOY 0.17 0.00 0.73 2009 Jan None captured Feb None captured Mar Black Crappie PYSL 0.25 0.00 0.54 Threadfin Shad Undetermined 0.42 0.00 1.23 YSL 21.76 0.00 44.90 PYSL 4.43 0.00 10.24 Darter YSL 0.09 0.00 0.29 Unidentifiable larvae Undetermined 0.29 0.00 0.98 White Perch YSL 3.93 0.00 9.91 PYSL 0.60 0.00 1.40 Yellow Perch PYSL 0.09 0.00 0.29 Apr Black Crappie PYSL 0.31 0.00 0.84 Gizzard Shad YSL 2.48 0.00 7.88 Threadfin Shad Undetermined 3.06 0.00 7.94 YSL 17.97 0.00 43.23 PYSL 14.72 1.37 28.06 Catastomidae YSL 0.10 0.00 0.35 PYSL 0.54 0.00 1.32 Cyprinidae YSL 0.33 0.00 1.11 PYSL 0.31 0.00 0.68 Lepomis sp. PYSL 0.11 0.00 0.38 White Perch YSL 15.55 1.08 30.02 PYSL 7.41 0.67 14.15 May Gizzard Shad YSL 2.99 0.00 7.57 Threadfin Shad YSL 17.91 0.00 41.43 PYSL 98.27 31.73 164.81 Catastomidae PYSL 0.49 0.00 1.09 Cyprinidae PYSL 0.37 0.00 0.83 Darter YSL 0.09 0.00 0.31_ PYSL 0.19 0.00 0.64 Lepomis sp. PYSL 0.92 0.00 2.65 White Perch YSL 0.46 0.00 1.54 PYSL 3.46 0.00 7.72 Jun Blue Catfish YOY 0.39 0.00 1.08 Brown Bullhead YOY 0.09 0.00 0.31 Channel Catfish YOY 0.92 0.00 3.09 Spottail Shiner YOY 0.10 0.00 0.33 Threadfin Shad YOY 0.09 0.00 0.31 Clupeidae YSL 0.71 0.00 2.37 PYSL 28.00 0.00 62.23 Cyprinidae PYSL 0.69 0.00 1.58 Lepomis sp. PYSL 0.52 0.00 1.34 White Catfish YOY 0.10 0.00 0.33 White Perch PYSL 0.29 0.00 0.99 Jul Blue Catfish Yearling 0.10 0.00 0.35 Clupeidae PYSL 1.35 0.40 2.30 Cyprinidae PYSL 0.10 0.00 0.35 Lepomis sp. PYSL 1.42 0.00 3.16 Aug Blue Catfish Yearling 0.21 0.00 0.86 Clupidae IPYSL 0.20 .. 0.00 1 0.85 Levomis sD.PYSL-.021 0 00 0 XQ PYS 000-- 089 .,-'. _ _ _21448.001 Summer Station Ichthyo Report.doc 9/28/09 13 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008 -AUGUST 2009 Table 3-1. Continued)

Footnotes:

a Undetermined

= specimen was damaged and lifestage could not be determined.

YSL = Yolk-sac larvae PYSL = Post yolk-sac larvae YOY = Young-of-the-year b Yearling = Age 1 b LCL = Lower 95% confidence limit.' UCL = Upper 95 % confidence limit.21448.001 Summer Station Ichthyo Report.doc 9/28/09 14 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Table 3-2. Average density (no./100 M 3) and percent composition of ichthyoplankton collected in Monticello Reservoir September 2008 through August 2009.Average Density Percent Species Lifestagea (no./100 M 3) Composition Black Crappie PYSL 0.02 0.2 Gizzard Shad YSL 0.16 2.2 Threadfin Shad Undetermined 0.10 1.4 YSL 1.70 22.8 PYSL 3.45 46.4 Catastomidae YSL <0.01 <0.1 PYSL 0.03 0.4 Clupeidae YSL 0.02 0.3 PYSL 0.87 11.6 Cyprinidae YSL 0.01 0.1 PYSL 0.04 0.6 Darter YSL 0.01 0.1 PYSL 0.01 0.1 Lepomis sp. PYSL 0.09 1.2 Unidentifiable larvae Undetermined 0.01 0.1 White Perch YSL 0.59 7.9 PYSL 0.35 4.7 Yellow Perch PYSL <0.01 <0.1 Footnotes:

Undetermined

= specimen was damaged and lifestage could not be determined.

YSL = Yolk-sac larvae, PYSL = Post yolk-sac larvae 21448.001 Summer Station Ichthyo Report.doc 9/28/09 15 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008 -AUGUST 2009 Table 3-3. Annual entrainment estimates for V.C. Summer Nuclear Station Units 2 and 3 under typical and maximum water withdrawal rates.Typical Flow (137.08 m 3/min) Maximum Flow (222.58 m 3/min)Species Lifestage' LCLb I Mean UCLc LCL Mean LCL Black Crappie Undetermined 0 0 YSL 0 0 PYSL 0 33,656 82,714 0 54,648 134,305 Total 0 33,656 82,714 0 54,648 134,305 Gizzard Shad Undetermined 0 0 YSL 0 329,828 929,431 0 535,549 1,509,139 PYSL 0 0 Total 0 329,828 929,431 0 535,549 1,509139 Threadfin Shad Undetermined 0 206,910 545,243 0 335,964 885,324 YSL 0 3,491,664 7,842,631 0 5,669,497 12,734,263 PYSL 2,022,666 7,156,168 12,373,370 3284250 11,619,638 20,090,929 Total 2,022,666 10,854,742 20,761,243 3,284,250 17,625,099 33,710,516 Catastomidae Undetermined 0 0 YSL 0 5,922 20,904 0 9,615 33,943 PYSL 0 61,962 144,742 0 100,610 235,020 Total 0 67,884 165,646 0 110,225 268,963 Clupeidae Undetermined 0 0 YSL 0 42,045 140,508 0 68,270 228,146 PYSL 24,196 1,752,968 3,877,847 39,287 2,846,335 6,296,551 Total 24,196 1,795,013 4,018,355 39,287 2,914,605 6,524,697 Cyprinidae Undetermined 0 0 YSL 0 19,542 65,608 0 31,731 106,530 PYSL 0 87,979 205,698 0 142,854 333,996 Total 0 107,521 271,306 0 174,585 440,526 Darter Undetermined 0 0 YSL 0 11,015 36,660 0 17,885 59,526 PYSL 0 11,627 39,408 0 18,878 63,988 Total 0 22,641 76,068 0 36,763 123,514 Lepomis sp. Undetermined 0 0 YSL 0 0 PYSL 0 193,349 512,018 0 313,945 831,375 Total 0 193,349 512,018 0 313,945 831,375 Unidentifiable Undetermined 0 17,746 59,724 0 28,814 96,975 Larvae YSL 0 0 PYSL 0 0 Total 0 17,746 59,724 0 28,814 96,975 White Perch Undetermined 0 0 YSL 64,205 11,89,484 2,478,440 104,251 1,931,393 4,024,301 PYSL 39,896 704,425 1,454,043 64,779 1,143,791 2,360,964 Total 104,100 1,893,908 3,932,483 169,030 3,075,183 6,385,265 Yellow Perch Undetermined 0 0 YSL 0 0 PYSL 0 5,507 18,040 0 8,942 29,291 Total 0 5,507 18,040 0 8,942 29,291 Total 2,150,962 15,321,796 30,827,028 3,492,567 24,878,358 50,054,566 Footnotes:

a Undetermined

= specimen was damaged and lifestage could not be determined.

YSL = Yolk-sac larvae PYSL = Post yolk-sac larvae b LCL = Lower 95% confidence limit.' UCL = Upper 95 % confidence limit.21448.001 Summer Station Ichthyo Report.doc 9/28/09 16 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008 -AUGUST 2009 APPENDIX 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-1 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. lchthyoplankton density data (no./100 m 3) from Monticello Reservoir, September 2008 through August 2009.Day Night All Density Density Density LCL UCL Year Month Species Lifestagea (no./100 m 3) (no./l00 m 3) (no./lO0 m 3) (no./100 m 3)b (no.d100 m 3)2 2008 Sep Black Crappie Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearline 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0.39 0.19 0.00 0.81 Yearling 0 0 0 Gizzard Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Threadfin Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Clupeidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearline 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-2 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All _ _Density Density Density LCL UCL Year Month Species Lifestagea (no./100 mn 3) (no./100 m 3) (no./100 m 3) (no./100 m 3)b (no./100 m 3 Lc Darter Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Lepomis sp. Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Unidentifiable Larvae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearlinc 0 0 0 0 0 0I I Oct Black Crappie Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-3 Normandeau Associates, .Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestagea 00 m 3) (no./100 m 3) (no./100 m 3) (no./100 m 3)b (no./100 m 3)c Gizzard Shad Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Threadfin Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Clupeidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Darter Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Lepomis sp. Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Unidentifiable Larvae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearlina 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-4 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestagea (no./100 in 3) (no./100 in 3) (no./100 in 3) (no./100 m 3)b (no./100 m 3 White Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 0- 0 0I- I I _ _ _Nov Black Crappie Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Gizzard Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Threadfin Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 00 0 0 Yearling 0 0 0 0 .0 0 ____(continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-5 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008 -AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species [Lifestage (no./100 m3) (no./100 mn 3) (no./100 m 3) (no./100 m 3)b (no./100 m 3)c Clupeidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Darter Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Lepomis sp. Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Unidentifiable Larvae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Dec Black Crappie Undetermined 0 0 0 YSL 0 0 0 1 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 I Yearling 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-6 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All _ _ _Density Density Density LCL UCL Year Month Speciies tage' (no./100 m 3) (no./100 mn3 1 (no./100 m 3) (no./100 m 3)b J(no./100 m 3 c Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 6 0 YOY 0 0 0 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Gizzard Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Threadfin Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0.35 0.17 0.00 0.73 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Clupeidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Darter Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Lepomis sp.Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0..e ... lino 0 0 0 Yerln 01 0__________

04- L -4L______ _ _ _ _(continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-7 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestagea (no./100 m 3) (no./100 m 3) (no./100 M 3) (no./100 m 3)T (no./100 m 3)c Unidentifiable Larvae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 2009 Jan Black Crappie Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Gizzard Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 1 1 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-8 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestage' (no./100 m 3) i (no./100 n 3) I(no./100 n 3) (no./100 mn 3)b (no.12t00 m 3)Threadfin Shad Undetermined I 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Clupeidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 0 0 0 PYSL. 0 0 0 YOY 0 0 0 Yearling 0 0 0 Darter Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Lepomis sp. Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Unidentifiable Larvae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 1 Yearling 0 0 0 1 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0.......rlino 0 0 0 0 0_______ 0 _______________.

_______(continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-9 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestage' (no./100 m 3) (no./100 m 3) (no./100 m 3) (no./100 m 3)b (no./100 m 3)c Feb Black Crappie Undetermined 0 0 0 YSL 0 0 1 0 1 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Gizzard Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Threadfin Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Clupeidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 1 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 0 0 0 1 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-10 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL J UCL Year Month Species Lifestage (no./100 i 3) (no./100 m 3) (no./100 m 3) (no./100 m 3)b (no./100 m 3)c Darter Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Lepomis sp. Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0_Yearling 0 0 0 Unidentifiable Larvae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Mar Black Crappie Undetermined 0 0 0 YSýL 0 0 0 PYSL 0.18 0.32 0.25 0.00 0.54 YOY 0 0 0 Yearling 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-11I Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All e Density Density Density LCL UCL!Year Month Species Lifestagea (no./100 m3) (no./100 m-) (no./100 M3) l(no./100 m3)b I(no./10 mli )c Gizzard Shad Undetermined 0 0 0 YSL 00 0 0 _ _ _PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Threadfin Shad Undetermined 0.69 0.15 0.42 0.00 1.23 YSL 27 16.52 21.76 0.00 44.90 PYSL 3.69 5.16 4.43 0.00 10.24 YOY 0 0 0 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Clupeidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Darter Undetermined 0 0 0 YSL 0.17 0 0.09 0.00 0.29 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Lepomis sp. Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Unidentifiable Larvae Undetermined 0 0.58 0.29 0.00 0.98 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Catfish Undetermined 0 0 0 YSL 0 1 0 1 0 1 PYSL 0 0 0 1 YOY 0 0 0 1 Yearling 0 0 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-12 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night 1 All Density Density Density LCL UCL Year Month Species Lifestage' J n./100 m 3)1 (no./100 m 3) (no./100 mI) (no./100 mI), (no./100 m 3 c White Perch Undetermined 0 0 0 YSL 1.9 5.95 3.93 0.00 9.91 PYSL 0.52 0.68 0.6 0.00 1.40 YOY 0 0 0 Yearling 0 0 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 0 0 PYSL 0.18 0 0.09 0.00 0.29 YOY 0 0 0 Yearlina 0 0 0 0 0 01I 4 -I Apr Black Crappie Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0.62 0.31 0.00 0.84 YOY 0 0 0 Yearling 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0.Gizzard Shad Undetermined 0 0 0 YSL 0 4.96 2.48 0.00 7.88 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Threadfin Shad Undetermined 1.98 4.15 3.06 0.00 7.94 YSL 7.85 28.09 17.97 0.00 43.23 PYSL 9.05 20.38 14.72 1.37 28.06 YOY 0 0 0 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0.21 0.1 0.00 0.35 PYSL 0.23 0.86 0.54 0.00 1.32 YOY 0 0 0 Yearling 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-13 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008 -AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year. Month Species Lifestage' (no./100 m 3) (no./100 m 3) (no./100 m 3) (no./100 m 3)b I(no./100 mSt Clupeidae Undetermined 0 0 0 YSL 0 1 0 0 1 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 0 0.66 0.33 0.00 1.11 PYSL 0 0.63 0.31 0.00 0.68 YOY 0 0 0 Yearling 0 0 0 Darter Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Lepomis sp. Undetermined 0 0 0 YSL 0 0 0 PYSL 0.23 0 0.11 0.00 0.38 YOY 0 0 0 Yearling 0 0 0 Unidentifiable Larvae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Perch Undetermined 0 0 0 YSL 4.08 27.03 15.55 1.08 30.02 PYSL 2.99 11.83 7.41 0.67 14.15 YOY 0 0 0 Yearling 0 0 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 00 00 YOY 0 0 0 Yearling 0 0 0___ F + + + F +May Black Crappie Undetermined 0 0 0 YSL 0 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-14 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestagea (no./100 m 3) (no./100 n 3) (no./100 m 3) (no./100 M 3 b (no./100 m 3)c Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Gizzard Shad Undetermined 0 0 0 YSL 2.3 3.69 2.99 0.00 7.57 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Threadfin Shad Undetermined 0 0 0 YSL 6.56 29.26 17.91 0.00 41.43 PYSL 52.55 143.99 98.27 31.73 164.81 YOY 0 0 0 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0.61 0.37 0.49 0.00 1.09 YOY 0 0 0 Yearling 0 0 0 Clupeidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 0. 0 0 PYSL 0 0.73 0.37 0.00 0.83 YOY 0 0 0 Yearling 0 0 0 Darter Undetermined 0 0 0 YSL 0 0.18 0.09 0.00 0.31 PYSL 0 0.38 0.19 0.00 0.64 YOY 0 0 0 Yearling 0 0 0 Lepomis sp.Undetermined 0 0 0 YSL 0 0 0 PYSL 0 1.85 0.92 0.00 2.65 YOY 0 0 0 Yearling 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-15 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestage' (no./100 m 3) (no./100 m 3) (no./100 mn 3) (no./100 m 3 b1 (no./100 m 3)c Unidentifiable Larvae Undetermined 0 0 0 YSL 0 0 1 0 1 PYSL 0 0 0 YOY 0 0 0 Yearlini, 0 0 0 White Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Perch Undetermined 0 0 0 YSL 0 0.92 0.46 0.00 1.54 PYSL 2.12 4.79 3.46 0.00 7.72 YOY 0 0 0 Yearling 0 0 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Jun Black Crappie Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0.77 0.39 0.00 1.08 Yearling 0 0 0 Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0.18 0.09 0.00 0.31 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 1.83 0.92 0.00 3.09 Yearling 0 0 0 Gizzard Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0.2 0.1 0.00 0.33 Yearline 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-16 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008 -AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestagea (no./100 m 3) (no./100 m 3) (no./100 n 3) (no./100 m 3)b (no./100 m 3 c Threadfin Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 YOY 0 0.18 0.09 0.00 0.31 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Clupeidae Undetermined 0 0 0 YSL 0 1.41 0.71 0.00 2.37 PYSL 11.83 44.17 28 0.00 62.23 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 0 0- 0 PYSL 0.2 1.18 0.69 0.00 1.58 YOY 0 0 0 Yearling 0 0 0 Darter Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Lepomis sp. Undetermined 0 0 0 YSL 0 0 0 PYSL 1.03 0 0.52 0.00 1.34 YOY 0 0 0 Yearling 0 0 0 Unidentifiable Larvae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0.2 0.1 0.00 0.33 Yearling 0 0 0 White Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0.59 0.29 0.00 0.99 YOY 0 0 0 Yearling 0 0 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY + 0 0 0 Yearline 0 0 0 0 0 0 ._________

________(continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-17 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestage' (no./100 m 3) (no./100 i 3) (no./100 mn 3) (no./100 m 3)h (no./100 m 3)c Jul Black Crappie Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0_ Yearling 0 0.21 0.1 0.00 0.35 Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Gizzard Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Threadfin Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Clupeidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0.67 2.03 1.35 0.40 2.30 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 1 0 1 0 0 1 PYSL 0 0.21 0.1 0.00 0.35 YOY 0 0 0 Yearling 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-18 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestage' (no./100 m 3) (no./100 m 3) (no./100 m 3) no./100 .3)b (no./100 m 3)c Darter Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Lepomis sp. Undetermined 0 0 0 YSL 0 0 0 PYSL 1.79 1.06 1.42 0.00 3.16 YOY 0 0 0 Yearling 0 0 0 Unidentifiable Larvae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 White Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 1 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 0t 0- 0-- -4 Aug Black Crappie Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Blue Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0.41 0.21 0.00 0.86 Brown Bullhead Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Channel Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-19 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestagea (no./100 m 3) (no./100 m 3) (no./100 m 3) (no./100 m 3)b (no./100 m 3)c Gizzard Shad Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Spottail Shiner Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Threadfin Shad Undetermined 0 01 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Catastomidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Clupeidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0.41 0.2 0.00 0.85 YOY 0 0 0 Yearling 0 0 0 Cyprinidae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Darter Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 .0 Lepomis sp. Undetermined 0 0 0 ..*YSL 0 0 0 PYSL 0.43 0 0.21 0.00 0.89 YOY 0 0 0 Yearling 0 0 0 Unidentifiable Larvae Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0'I White Catfish Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-20 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 1. (Continued)

Day Night All Density Density Density LCL UCL Year Month Species Lifestagea (no./100 n 3) (no./100 M 3) (no./100 m 3) (no./100 m3)b (no./100 m3), White Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 YOY 0 0 0 Yearling 0 0 0 Yellow Perch Undetermined 0 0 0 YSL 0 0 0 PYSL 0 0 0 0 YOY 0 0 0 1 Yearling 0 0 0 Footnotes:

a Undetermined

= specimen was damaged and lifestage could not be determined.

YSL = Yolk-sac larvae PYSL = Post yolk-sac larvae YOY = Young-of-the-year Yearling = Age I b LCL = Lower 95% confidence limit.' UCL = Upper 95 % confidence limit.21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-21 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 2. Monthly larval entrainment estimates for V.C. Summer Nuclear Station Units 2 and 3 under typical and maximum water withdrawal rates.1I MTypical Flow (137.08 m 3/min) Maximum Flow (222.58 m 3/min)Year Month Species Lifestage' I LCLb I Mean I UCLC LCLb I Mean I LCL'2008 September None 0 0 October None 0 0 November None 0 0 December None 0 0 2009 January None 0 0 February None 0 0 March Black Crappie Undetermined 0 0 YSL 0 0 PYSL 0 15298 32977 0 24840 53545 Threadfin Shad Undetermined 0 25701 75261 0 41731 122203 YSL 0 1331549 2747415 0 2162067 4461042 PYSL 0 271083 626458 0 440164 1017195 Darter Undetermined 0 0 YSL 0 5507 17807 0 8942 28914 PYSL 0 0 Unidentifiable Undetermined 0 17746 59724 0 28814 96975 larvae YSL 0 0 PYSL 0 0 White Perch Undetermined 0 0 YSL 0 240487 606295 0 390484 984456 PYSL 0 36716 85419 0 59616 138696 Yellow Perch Undetermined 0 0 YSL 0 0 PYSL 0 5507 18040 0 8942 29291 March Total 0 1949593 4269396 0 3165600 6932317 April Black Crappie Undetermined 0 0 YSL 0 0 PYSL 0 18358 49738 0 29808 80760 Gizzard Shad Undetermined 0 0 YSL 0 146862 466394 0 238463 757294 PYSL 0 0 Threadfin Shad Undetermined 0 181209 469982 0 294233 763121 YSL 0 1064158 2560095 0 1727897 4156887 PYSL 80881 871697 1661963 131328 1415395 2698568 Catastomidae Undetermined 0 0 YSL 0 5922 20904 0 9615 33943 PYSL 0 31978 78115 0 51923 126837 Cyprinidae Undetermined 0 0 YSL 0 19542 65608 0 31731 106530 PYSL 0 18358 40168 0 29808 65222 Lepomis sp. Undetermined 0 0 YSL 0 0 PYSL 0 6514 22521 0 10577 36568 White Perch Undetermined 0 0 YSL 64205 920849 1777877 104251 1495203 2886781 PYSL 39896 438810 837806 64779 712505 1360366 April Total 1849811 37242551 8051172 3003561 60471601 13072876 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-22 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 2. (Continued) 11 MTypical Flow (137.08 m 3/min) Maximum Flow (222.58 m 3/min)Year Month [Species Lifestage LCLb I Mean I UCLC LCLb ]Mean I LCLC 2009 May Gizzard Shad Undetermined YSL PYSL 0 0 182966 0 463038 0 0 297086 0 751845 Threadfin Shad Undetermined 0 0 YSL 0 1095958 2535120 0 1779532 4116334 PYSL 1941785 6013388 10084948 3152922 9764079 16375166 Catastomidae Undetermined 0 0 YSL 0 0 PYSL 0 29984 66626 0 48686 108183 Cyprinidae Undetermined 0 0 YSL 0 0 PYSL 0 22641 50765 0 36763 82429 Darter Undetermined 0 0 YSL 0 5507 18853 0 8942 30613 PYSL 0 11627 39408 0 18878 63988 Lepomis sp. Undetermined 0 0 YSL 0 0 PYSL 0 56297 162197 0 91411 263363 White Perch Undetermined YSL PYSL 0 0 0 28149 211726 94267 472186 0 0 0 45705 343785 153064 766699 May Total 1941785 7658243 13987409 3152922 12434868 22711683 June Clupeidae Undetermined 0 0 YSL 0 42045 140508 0 68270 228146 PYSL 0 1658120 3685195 0 2692328 5983737 Cyprinidae Undetermined 0 0 YSL 0 0 PYSL 0 40861 93506 0 66347 151828 Lepomis sp. Undetermined 0 0 YSL 0 0 PYSL 0 30794 79128 0 50000 128482 White Perch Undetermined 0 0 YSL 0 0 PYSL 0 17173 58632 0 27885 95203 June Total 0 1788993 4056969 0 2904829 6587395 July Clupeidae Undetermined 0 0 YSL 0 0 PYSL 24196 82610 140712 39287 134136 228478 Cyprinidae Undetermined 0 0 YSL 0 0 PYSL 0 6119 21258 0 9936 34518 Lepomis sp. Undetermined 0 0 YSL 0 0 PYSL 0 86893 193423 0 141091 314066 July Total 24196 175623 355394 39287 285162 577061 August Clupeidae Undetermined 0 0 YSL 0 0 PYSL 0 12239 51940 0 19872 84337 Lepomis sp. Undetermined 0 0 YSL 0 0 PYSL 0 12850 54749 0 20866 88897 Au2ust Total 01 250891 106689 0 40737 173234-' 6 4 U U .A. I. J (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-23 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 2. (Continued)

Typical Flow (137.08 m 3/min) Maximum Flow (222.58 m 3/min)Yearlonth Species Lifestage LCLb Mean I UCLC LCLb [ Mean I LCLc All Black Crappie Undetermined 0 0 YSL 0 0 PYSL 0 33656 82714 0 54648 134305 Gizzard Shad Undetermined 0 0 YSL 0 329828 929431 0 535549 1509139 PYSL 0 0 Threadfin Shad Undetermined 0 206910 545243 0 335964 885324 YSL 0 3491664 7842631 0 5669497 12734263 PYSL 2022666 7156168 12373370 3284250 11619638 20090929 Catastomidae Undetermined 0 0 YSL 0 5922 20904 0 9615 33943 PYSL 0 61962 144742 0 100610 235020 Clupeidae Undetermined 0 0 YSL 0 42045 140508 0 68270 228146 PYSL 24196 1752968 3877847 39287 2846335 6296551 Cyprinidae Undetermined 0 0 YSL 0 19542 65608 0 31731 106530 PYSL 0 87979 205698 0 142854 333996 Darter Undetermined 0 0 YSL 0 11015 36660 0 17885 59526 PYSL 0 11627 39408 0 18878 63988 Lepomis sp. Undetermined 0 0 YSL 0 0 PYSL 0 193349 512018 0 313945 831375 Unidentifiable Undetermined 0 17746 59724 0 28814 96975 larvae YSL 0 0 PYSL 0 0 White Perch Undetermined 0 0 YSL 64205 1189484 2478440 104251 1931393 4024301 PYSL 39896 704425 1454043 64779 1143791 2360964 Yellow Perch Undetermined 0 0 YSL 0 0 PYSL 0 5507 18040 0 8942 29291 TOTAL 2,150,962 15,321,796 30,827,028 3,492,567 24,878,358 50,054,566 Footnotes:

a Undetermined

= specimen was damaged and lifestage could not be determined.

YSL = Yolk-sac larvae PYSL = Post yolk-sac larvae b LCL = Lower 95% confidence limit.UCL = Upper 95 % confidence limit.21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-24 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008 -AUGUST 2009 Appendix Table 3.Water quality data associated with ichthyoplankton sampling in Monticello Reservoir, September 2008 through August 2009.Water Dissolved Depth Temperature Oxygen Date Diel Time (m) (°C) (mg/l)09/11/08 day 17:55 0.9 28.2 6.2 3.0 27.9 5.2 5.8 27.5 3.9 09/11/08 night 23:20 0.9 28.5 6.1 4.0 27.9 5.3 7.3 27.4 3.3 10/16/08 day 16:00 0.9 24.5 7.8 12.2 23.6 6.2 24.1 23.4 6.1 10/16/08 night 21:55 0.9 24.3 7.1 13.7 23.5 6.1 27.4 23.3 5.3 11/20/08 day 15:40 0.9 17.4 9.2 12.2 16.9 9.0 24.4 16.8 8.9 11/20/08 night 20:15 0.9 16.9 9.3 12.2 16.6 9.1 24.4 16.4 9.1 12/16/08 day 15:30 0.9 15.3 10.0 15.2 13.0 9.5 29.0 12.0 9.5 12/16/08 night 20:10 0.9 14.1 10.0 15.2 13.2 9.4 29.0 12.0 9.4 01/17/09 day 14:45 0.9 12.2 11.3 13.7 11.6 11.2 27.4 11.3 11.2 01/17/09 night 20:30 0.9 11.8 11.3 13.7 11.6 11.1 29.0 10.6 11.2 02/21/09 day 15:50 0.9 12.1 11.3 13.7 10.6 10.6 27.4 10.2 10.0 02/21/09 night 21:00 0.9 11.4 11.2 13.7 10.4 10.7 29.0 9.9 10.3 03/14/09 day 16:50 0.9 15.4 10.7 13.7 11.7 10.6 29.0 10.5 10.4 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-25 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 3. (Continued)

Dissolved Depth Temperature Oxygen Date Diel Time (m) (0 C) (mrg/I)03/14/09 night 20:10 0.9 15.0 11.1 13.7 11.3 11.1 29.0 10.5 10.3 03/28/09 day 17:00 0.9 15.5 9.8 13.7 13.2 9.6 29.0 11.2 9.0 03/28/09 night 22:25 0.9 15.7 9.6 13.7 13.0 9.3 27.4 11.1 8.9 04/18/09 day 17:40 0.9 18.3 11.0 7.6 15.7 9.3 15.2 15.1 8.8 04/18/09 night 22:35 0.9 18.0 10.5 7.6 15.5 9.1 15.2 15.1 8.6 04/25/09 day 17:02 0.9 20.5 9.6 7.6 17.1 8.8 15.2 15.9 8.5 04/25/09 night 22:55 0.9 19.3 8.9 7.6 17.5 8.7 15.2 16.2 8.4 05/09/09 day 17:20 0.9 22.6 8.8 7.6 19.7 7.9 15.2 18.3 7.5 05/09/09 night 22:45 0.9 22.9 8.8 7.6 20.1 7.7 15.2 18.9 7.5 05/23/09 day 17:00 0.9 22.1 7.8 7.6 21.4 7.6 15.2 20.7 7.1 05/23/09 night 23:00 0.9 22.3 8.4 7.6 21.4 7.9 15.2 20.8 7.2 06/06/09 day 18:07 0.9 26.9 9.6 7.6 24.9 7.8 15.2 22.8 6.7 06/06/09 night 23:09 0.9 26.2 9.2 7.6 24.0 7.3 15.2 22.9 6.6 (continued) 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-26 Normandeau Associates, Inc.

MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 3. (Continued)

Dissolved Depth Temperature Oxygen Date Diel Time (m) (°C) (mg/I)06/20/09 day 18:00 0.9 30.8 10.3 7.6 25.9 6.7 15.2 25.4 5.4 06/20/09 night 23:15 0.9 30.0 9.9 7.6 26.0 6.1 15.2 25.4 5.2 07/11/09 day 17:45 0.9 29.4 8.7 7.6 27.7 6.0 15.2 27.3 4.3 07/11/09 night 23:17 0.9 29.1 9.3 7.6 27.7 5.4 15.2 27.5 4.7 07/25/09 day 17:40 0.9 29.7T 7.7 7.6 28.4 5.4 15.2 27.9 4.0 07/25/09 night 23:10 0.9 29.7 8.4 7.6 28.2 5.0 15.2 27.8 3.7 08/22/09 night 19:00 0.9 29.9 7.4 7.6 29.3 5.6 15.2 28.9 3.7 08/22/09 night 22:45 0.9 29.6 6.9 7.6 29.1 4.4 1 _ 1 _ 1_ 15.2 29.0 3.5 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-27 Normandeau Associates, Inc.

I, MONTICELLO RESERVOIR ICHTHYOPLANKTON STUDIES SEPTEMBER 2008- AUGUST 2009 Appendix Table 4. Environmental conditions associated with ichthyoplankton sampling in Monticello Reservoir September 2008 through August 2009.Cloud Air Temp Cover Wind Wind Speed Date Diel Time (°C) (%) Precipitation Direction (mph) Wave Height 09/11/08 day 17:55 25 90-100 none east 8-11 light chop night 23:20 23 90-100 light rain east 8-11 light chop 10/16/08 day 16:00 29 1-9 none west 8-11 light chop night 21:55 23 10-19 none no wind 0-7 calm 11/20/08 day 15:40 17 1-9 none west 0-7 calm night 20:15 13 1-9 none south 0-7 calm 12/16/08 day 15:30 19 70-79 none no wind 0-7 calm night 20:10 16 30-39 none south 0-7 calm 01/17/09 day 14:45 4 1-9 none south 8-11 light chop night 20:30 5 90-100 none south 8-11 light chop 02/21/09 day 15:50 14 1-9 none south 0-7 calm night 21:00 8 20-29 none south 0-7 calm 03/14/09 day 16:50 8 90-100 light rain east 8-11 light chop night 20:10 8 90-100 light rain east 0-7 light chop 03/28/09 day 17:00 15 90-100 heavy rain north 12-16 heavy chop night 22:25 15 50-59 none north 0-7 calm 04/18/09 day 17:40 23 90-100 none west 0-7 light chop night 22:35 17 10-19 none no wind 0-7 calm 04/25/09 day 17:02 30 20-29 none south 8-11 calm night 22:55 23 10-19 none south 0-7 calm 05/09/09 day 17:20 28 70-79 none south 12-16 light chop night 22:45 24 10-19 none no wind 0-7 calm 05/23/09 day 17:00 24 90-100 none east 8-11 light chop night 23:00 22 90-100 none south 0-7 calm 06/06/09 day 18:07 28 60-69 none east 8-11 calm night 23:09 23 60-69 none east 8-11 calm 06/20/09 day 18:00 35 20-29 none west 8-11 calm night 23:15 28 10-19 none west 0-7 calm 07/11/09 day 17:45 33 60-69 none west 0-7 calm night 23:17 25 30-39 none no wind 0-7 calm 07/25/09 day 17:40 34 20-29 none south 0-7 calm night 23:10 28 10-19 none no wind 0-7 calm 08/22/09 night 19:00 31 50-59 none west 0-7 calm night 22:45 28 50-59 none north 8-11 light chop 21448.001 Summer Station Ichthyo Report.doc 9/28/09 A-28 Normandeau Associates, Inc.

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