Text

NPDES Discharge Monitoring Rept,Jan-Mar 1999. with
	ML20205R188
Person / Time
Site:	Sequoyah
Issue date:	03/31/1999
From:	Bodine D; TENNESSEE VALLEY AUTHORITY
To:	Haskin E; TENNESSEE, STATE OF
References
	NUDOCS 9904220208
	Download: ML20205R188 (103)
	v • d • e

Ed Tennessee Valley Authority, Post Offce Box 2000, Soddy-Daisy, Tennessee 27379 2000

' April 14, 1999 State of Tennessee Department of Environment and Conservation Division of Water Pollution Contro!

401 Church Street,6th Floor Annax Nashville, Tennessee 37243-1534 Attention: Ms. Evelyn Haskin, Enforcement & Compliance

Dear Ms. Haskin:

TENNESSEE VALLEY AUTHORITY-DISCHARGE MONITORING REPORT HARD COPlES FOR SEQUOYAH NUCLEAR PLANT (SON)

Pleaso find enclosed the First Quarter 1999 DMR for SON.

Please contact me at (423) 843-6700 or Diedre Nida at (423) 843-6711 if you have any questions or comments.

Sincerely,

?

Debby Bodine Environmental Supervisor Sequoyah Nuclear Plant Enclosure cc (Enclosure):

Chattanooga Environmental Assistance Center Division of Water Pollution Control Stde Office Building, Suite 550 540 McCallie Avenue l

Chattanooga, Tennessee 37402-2013 I

U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington,D.C. 20555

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Quarterly DMR Data for 1/99,2/99, and 3/99 CCW Channel Discharc e Date:

Diesel Range Total Water Comments:

Organics (mg/L)

Hydrocarbons (mg/L) 1/6/99

< 0.1

< 1.0 1/13/99

< 0.1

< 1.0 1/20/99

< 0.1 1.2 See (1) below 1/20/99

< 1.0 See (1) below l

1/27/99

< 0.1

< 1.0 2/3/99

< 0.1

< 1.0 2/10/99

< 0.1

< 1.0 2/17/99

< 0.1

< 1.0 2/24/99

< 0.1

< 1.0 3/3/99

< 0.1

< 1.0 3/10/99

< 0.1

< 1.0 3/17/99

< 0.1

< 1.0 3/24/99

<. 0.1

< 1.0 3/31/99

< 1.0

< 1.0 (1) The original sample submitted for 1/20/99 was followed up by a "back-up" sample that was collected simultaneously with the first sample. There was a possible laboratory error in the analysis of the first sample.

Recovery Trench Discharge Data Data:

Diesel Range Total Water Comments Organics (mg/L)

Hydrocarbons (mg/L) 1/6/99

< 0.1

< 1.0 l

1/13/99 0.3

< 1.0 1/20/99

< 0.1

< 1.0 1/27/99

< 0.1

< 1.0 2/3/99

< 0.1

< 1.0 2/10/99

< 0.1

< 1.0 2/17/99

< 0.1

< 1.0 2/24/99

< 0.1

< 1.0 3/3/99

< 0.1

< 1.0 3/10/99

< 0.1

< 1.0 3/17/99

< 0.1

< 1.0

)

3/24/99 0.1

< 1.0 1

3/31/99 0.2

< 1.0

i I

March 31,1999 Debra J. Bodine, OPS 2B-SQN j

SEQUOY AH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NOc TN0026450, FEBRUARY,1999 REPORT Attached are two copies of the subject report for submission to the state of Tennessee with the DMR package you submit for SQN. The repon provides results of compliance tests required under the SQN NPDES permit.

SQN Outfall 101 samples, collected February 22-28, were not toxic to daphnids (96-h LC3o > 100 percent, IC25, >100 percent), however, exposure of fathead minnows to Outfall 001, intake, and upstream samples resulted in sporadic monality throughout the test. Although the 25% and 80% concentrations of Outfall 101 showed statistically significant reductions in survival, the test was accepted as a pass by TDEC since the compliance concentration of 48.98%

exhibited no toxicity.

Call me at (256) 386-2755,if you have any questions or comments following you.

O review f the repon.

cp.2 As Cynthia L. Russell Biologist Environmental Engineering Services-West CTR 2P-M j

l CLR l

Attachment cc '(Attachment):

Files, ER&S, CEB IB-M sqn-0299m. doc 0

l TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

1) Facility / Discharger: Seouovah Nuclear Plant /TVA Report Date: March 31.1999

2) County / State: Hamilton / Tennessee

3) NPDES Permit #: TN0026450

4) Type of Facility: Nuclear-fueled electric ceneratine olant

5) Design Flow (MGD): 3.266 i

6) Receiving Stream: Tennessee River (TRM 483 6)

7) IQ20: 2.992.4

8) Outfall Tested: 101

9) Dates Sampled: 02/22-28/99

10) Flow on day (s) sampled (MGD): 1616 1622 1630 1141 1604 1606 1598

11) Pertinent site conditions: No unusual conditions reoorted.*

12) Test Dates: 02/23/99-0"4/02/99

13) Test Type: Short-term Chronic-Definitive

14) Test Species: Fathead Minnows (Pimephalespromelas)

Daohnids (Ceriodanhnia dubia)

15) Concentrations Tested (%): Outfall 101 25 39.2 49 80 100 Intake 100 Upstream 100 i

16) Permit Limit Endpoint (%): Outfall 101 96-hr LC<n = 100 NOEC= 48.98

17) Test Results (%): Qutfall 101 Pimephalespromelas 96-hr LCso > 100 NOEC (crowth) = 100 Ccriodaphnia dubia:

96-hr LC<o > 100 NOEC (IC,d > 100

18) Facility

Contact:

Debra 1 Bodine

19) Phone #: (423) 843-6700

20) Consultant / resting Lab Name: Commonwealth Technolocv. Inc. (CTI)

21) Lab

Contact:

CT1 - Paul W. Patterson

22) Phone #: (606) 276-3506

23) TVA

Contact:

Cynthia L Russell

24) Phone #: (256) 386-2755

Production / operation data will be provided in the event toxicity exceeds permit limits

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25) Notes Samples from Outfall 101 exhibited no toxicity to C. dubia based on the applicable permit limits.

)

Testing with fathead minnows resulted in sporadic monality throughout the test, resulting in a dose response which did not meet the requirements for use of point estimt: tion to determine an ICu based NOEC (i.e. "the response was NOT monotonically ncn-increasing, where the mean response for each higher concentration was less than or equal to the mean response for the previous concentration"). Hypothesis based analyses were, therefore, selected to analyze the results of this test (EPA -600-4-91-002).

The compliance endpoint of 48.98% (49%) showed no statistical difference from contmls in survival or growth. Survival in the 25% concentration was, however, found to be statistically less than controls, as was the 80% treatment. Growth was not sig sficantly reduced from controls in any effluent concentration.

Upstream and raw water intake samples showed similar pattems of mortality and also showed statistical differences from test controls when analyzed using the Homoscedastic t-Test, thereby demonstrating ambient water toxicity of some sort. Survival and growth were reduced significantly in the Upstream samples and growth was significant in the Intake samples.

We believe the random occurrence of mortality throughout the test and the resulting statistically significant reductions from controls were related to ambient receiving stream water used for cooling, NOT toxicity present in SQN Outfall 101. Microbial pathogens Q

present in the river are a likely cause of this type of highly variable survival response.

The test was accepted as a pass by Chris Moran, Tennessee Department of Environment arid Conservation, on March 9,1999, following discussions with Jennifer Moses, TVA's i

Senior Toxicologist, since the compliance concentration exhibited no significant j

differences from controls.

O 2

METHODS

SUMMARY

Samoles O

>> s m vita 8 v oiat: outreii toi

2) Sample Type: Grab

3) SampleInformation:

Date/ Time Date/ Time.

Arrival Initial Dateffime ID Collected Received Temp.

TRC*

Used (MM-DD/ rime, (MM-DD/ Time)

( C)

(mg/L)

(MM-DD/ Time) 101 02-22/0948 EST 02-23/1000 EST 1.0

< 0.02 02-23/1600 EST i

Intake 02-22/0930 EST 02-23/1000 EST 1.0

< 0.02 02-23/1600 EST Upstream 02-22/1014 EST 02-23/1000 EST 1.0

< 0.02 02-23/1600 EST 101 02-23/0728 EST 02-24/0940 EST 1.0

< 0.02 02-24/1600 EST Intake 02-23/0857 EST 02-24/0940 EST 1.0

< 0.02 02-24/1600 EST Upstream 02-23/0817 EST 02-24/0940 EST 1.0

< 0.02 02-24/1600 EST 4

101 02-24/0739 EST 02-25/1005 EST 1.0

< 0.02 02-25/1500 EST Intake 02-24/0943 EST 02-25/1005 EST 1.0

< 0.02 02-25/1500 EST Upstream 02-24/0850 EST 02-25/1005 EST 1.0

< 0.02 02-25/1500 EST 101 02-25/0733 EST 02-26/0925 EST 1.0

< 0.02 02-26/1445 EST 02-27/1400 EST Intake 02-25/1023 EST 02-26/0925 EST 1.4

< 0.02 02-26/1445 EST 02-27/1400 EST Upstream 02-25/1103 EST 02-26/0925 EST 0.6

< 0.02 02-26/1445 EST 02-27/1400 EST 101 02-26/0804 EST 03-01/0935 EST Discardedt Intake 02-26/0940 EST 03-01/0935 EST Discardedt Upstream 02-26/0828 EST 03-01/0935 EST Discardedt 101 02-27/0753 EST 02-27/1443 EST 0.8

< 0.02 02-28/1430 EST Intake 02-27/0825 EST 02-27/1443 EST 0.6

< 0.02 02-28/1430 EST Upstream 02-27/0737 EST 02-27/1443 EST 0.5

< 0.02 02-28/1430 EST 101 02-28/0801 EST 02-28/1505 EST 3.0

< 0.02 03-01/1500 EST Intake 02-28/0832 EST 02-28/1505 EST 3.0

< 0.02 03-01/1500 EST Upstream 02-28/0735 EST 02-28/1505 EST 3.0

< 0.02 03-01/1500 EST

' Total residual chlorine.

iSamples collected on 02/26/99 were not received in time for test renewal on 02/27/99. Therefore, the samples collected on 02/25/99 were used to renew the tests on both 02/26 and 02/27.

4) Sample manipulation: Samples were warmed to test temperature (25 C) in a warm water bath.

3

Test Orcanisms Pimephalespromelas Ceriodaphnia dubia

)

1) Source:

In-house Culture In-house Culture

2) Age:

< 24 hours2.777778e-4 days 0.00667 hours 3.968254e-5 weeks 9.132e-6 months

< 24 hours2.777778e-4 days 0.00667 hours 3.968254e-5 weeks 9.132e-6 months Test Method Summary (See Appendix A for additionalinfonnation)

Pimephalespromelas Ceriodaphnia dubia

1) Test Conditions Static. renewal Static.fenew31

2) Test Duration 7 days _

Until at leas: 00% of control females have 3 broods

3) Dilution / Control "9:1" Moderatelv Hard "9:1" Moderatelv Hard Water Reconstituted Water Reconstituted Water

4) Number Replicates 4

10 f) Animals per Replicate 10 1

6) Test Initiation 02-23-99/l100 EST 02-23-99/1600 ES_I (Date/ Time)

,}'

7) Test Termination 03-02-99/1100 EST 03-02-99/1500 EST (Date/ Time)

8) Test Temperature Mean = 25.0 C Mean = 24.7 C (24.2 C-25.3 C)

(24.0 C-25.3 C)

9) Physical / Chemical Measurements: Hardness, alkalinity, and conductivity were measured at the laboratory in each 100 percent sample. Temperature was measured in each test solution daily. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10) Statistics: Fathead minnow statistics followed EPA's statistical flow chart using SAS based programs. The test for assumptions showed that Steel's Many-one Ranked Test was the correct test for survival and Dunnett's Test was the correct test for sublethal (growth) effects.

Stati!; tics for daphnids were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).

Homoscedastic t-Tests were used to compare intake and upstream data to control.

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4

(

TOXICITY TEST RESULTS (See Appendix C for Bench Sheets)

1) Results of a Pimevhales promelas Chronic 7-d Toxicity Test (Genus)

(Species)

(Type / Duration)

Conducted 02/23/99 03/02/99 Using Efiluent From Outfall 10'

,)

(mm/dd/yy)

(number)

Percent Surviving Test Solutions (time intervals used - days) 1 2

1 4*

5 6

7 Control 10LQ 199 10Q 19qQ J0Q Q

10Q 109 q

25% Effluent 19Q 10.Q 100 100 85 78 71t 39.2% Effluent 100 100 M

95 88 88 88 49% Effluent 10Q 1QQ IqQ Iqq 90 90 88 Q

80% Effluent 10Q J09 19Q 199 88 70 68t 100% Effluent 10Q IQQ 1qq 1 90, 9 1QQ 93 88 Q

Intake 100 100 100 1QQ 93 85 83 Upstream 102 IQQ IQQ Q1 80 70 65

96-Hour LC 0 Value > 100%

Calculated TU Estimates 3<l.0TUa 5

95% Confidence Limits Permit Limit 1.0 TUa Upper Limit HA Lower Limit NA Mean Dry Weight (mg)

Test Solutions (Replication) i 1

2 1

4 hie _an Control 2,562 0.576 0.604 0.541 0.571 6

25% EfIluent 0.346 0.361 0.289 0.072 0.267 5

39.2% Effluent 0.360 0.555 0.541 0.252 0.427 49% Effluent 0.444 0.507 0.554 0.291 0.449 80% Effluent 0.195 0.431 0.451 0 333 0 352 5

100% Effluent 0.320 0.384 0.424 0.502 0.408 Intake 0.517 0.487 0.443 0.191 0.410 Upstream 0.209 0.362 0 385 0 407 0 341 NOEC Value = 100%

Calculated TU Estimates 1.0 TUc 3

i 95% Confidence Limits Permit Limit 2.0 TUc Upper Limit = NA Lower Limit = HA tStatistically less than control based on hypothesis testing.

NOTE: TUa = 100/LC50; TUc = 100/NOEC SNot included in growth analyses.

5

1

2) Results of a ceriodaphnia dubia Chronic / 7 day (3-brood) Toxicity Test (Genus)

(Species)

(Type / Duration)

')

i Conducted 02/23/99 - 03/02/99 Using EEuent From Outfall 101 (mm/dd/yy) (mm/dd/yy)

(number)

Percent Surviving Test Solutions (time intervals used - days)

{

i 1

2 1

e 1

6 2

Control IQQ 1QQ IQQ 109 LQQ 100 10Q 0

25% Emuent 1QQ 1QQ 1qq 100 100 109 1QQ 39.2% Emuent LQQ 10Q 10Q 109 100 100 Q

102 0

49% Emuent 1QQ LQQ LQQ 109 1QQ IQQ IQQ 80% Emuent 102 IQQ IQQ 100 100 100 100 100% Emuent 100 100 100 100 100 100 100 Intake 109 120 102 IQQ igg 120 1QQ

)

Upstream 1QQ 1QQ 1QQ 1QQ 109 LQQ LQQ

'96-Hont LC3a Value > 100%

Calculated TU Estimates t < l.0 TUa 95% Confidence Limits Permit Limit 1.0 TUa Lower Limit NA Reproduction (# ofyoung/ female during test) Data Test Solutions Replicates 1

2 1

4 5

6 2

8 Q

LQ Mean Control M

M D

11 M

ll 1h M

M H

lik 25% Emuent 11 35 M

34 M

28 M

H IQ 21 212 39.2% Emuent 22 LQ M

24 21 M

H H

31 22 29 9 49% Emuent 21 M

M M

22 H

12 21 29 29 30.1 80% Effluent H

30 30 34 34 29 21 M

31 20 30.2 100% Effluent 22 IQ 0

29 32 28 29 32 H

IQ 27.6 Intake M

21 32 M

22 12 24 36 H

21 112 Upstream 37 34 32 33 33 30 30 33 35 35 33.2 ICu Value > 100%

Calculated TU Estimates ' <10 TUc 95% Confidence Limits Permit Limit 2.0 TUc Upper Limit N_A Lower Limit NA HOTE: TUa = 100/LCso; TUc = 100/ ICs Q

6

I.

REFERENCE TOXICANT TEST RESULTS (See Appendixes A and C)

Species Date Time Duration Toxicant Results (LC5o/ICu)

P/mephalespromelas 02-12-99 1100 7 days Nacl 2411 mg/L (ICu)

Ceriodophnia dubia 02-12-99 1400 7 days Nacl-1533 mg/L (ICu) l l

l PHYSICAL / CHEMICAL

SUMMARY

1) Peninent Chemical Data and Summary of Application

2) Initial and Final Chemistry for Fathead Minnow 7-day and Daphnid 3-brood Chronic Tests.

See Summary Table in Appendix B

SUMMARY

/ CONCLUSIONS Samples from Outfall 101 exhibited no toxicity to fathead minnows or C. dubia based on the applicable statistical analyses and pennit limits.

0

~

O 7

1 Appendix A ADDITIONAL TOXICITY TEST INFORMATION G.

SUMMARY

OF METHODS

1) Pimephalespromelas Tests were conducted according to EPA /600/4-91/002 (July 1994) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL polystyrene tumblers, each containing 250 mL of test solution. [2]

2) Ceriodaphniadubia Tests were conducted according to EPA /600/4-91/00 ;'W;y 1994) using ten replicates, each containing one test organism, per trec m m Test vessels consisted of 30-mL polystyrene cups, each containing 15 mL of test Nbtion. [2]

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1) Pimephalespromelas C. dubia laboratory culture medium ("9:l" moderately hard reconstituted water) prepared according to CTI SOP 3040 was used for control and dilution water. The l

water consists of 9 parts moderately hard reconstituted water and 1 part mineral

)7 water. Experience has shown that the daphnids cultured in water without the mineral water addition are stressed. This stress is eliminated through the use of mineral water.

i For consistency, this same water is used for control / dilution water in P. promelas tests. [3]

2) Ceriodaphniadubia Laboratory culture medium ("9:1" moderately hard reconstituted water) prepared according to CTI SOP 3040 was used for control and dilution water. The water consists of 9 parts moderately hard reconstituted water and 1 part mineral water. Experience has shown that the daphnids cultured in water without the mineral water addition are stressed. This stress is eliminated through the use of mineral water. [3]

i 8

DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS h,

1) Pimephalespromelas None

2) Ceriodaphniadubia Laboratory culture medium consisted of"9:l" moderately hard reconstituted water prepared according to CTI SOP 3040. The water consists of 9 parts moc'erately hard reconstituted water and 1 pan mineral water. Experience has shown that the daphnids cultured in water without the mineral water addition are stressed. This stress is eliminated through the use of mineral water. [3]

PHYSICAL AND CHEMICAL METHODS

1) Reagents, Titrants, Buffers, etc.: All chemicals were cenified products used before expiration dates (where applicable).

2) Instruments: All identification, service, and calibration information pertaining to CTI laboratory instruments is recorded in calibration and maintenance log books.

3) Temperature was measured using a Labcraft digital NIST-traceable thermometer

(}

according to CTI SOP 3203. [3]

4) Dissolved oxygen was measured using a Jenway 3410 Electrochemistry Analyzer. The instrument was calibrated and readings were made according to CTI SOP 3187. [3]

5) The pH was measured using a Jenway 3410 Electrochemistry Analyzer equipped with an Orion combination electrode. The instrument was calibrated and readings were made according to CTI SOP 3195. [3]

6) Conductance was measured using a Jenway 3410 Electrochemistry Analyzer. The instrument was calibrated and readings were taken according to CTI SOP 3183. [3]

7) Alkalinity was measured using Standard Methods Titration Method 2320 B or EPA method 310.2 on a Lachat Colorimetric Autoanalyzer equipped with Quickchem AE Software. [4,5]

8) Hardness was measured using Standard Methods EDTA Titrimetric Method 2340 C or EPA Inethod 130.1 on a Lachat Colorimetric Autoanalyzer equipped with Quickchem AE Software. [4,5]

9) Total residual chlorine veas determined using the DPD Fen ous Titrimetric Method according to CTI SOP 3210. [3]

9

m OUALITY ASSURANCE

,]

'J

,~

Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage; glassware preparation; test organism culturing / acquisition and acclimation; test organism handling during test; and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA /600/4-91/002. [2] Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1) Test Type: 7-day chronic tests with results expressed as ICu values in mg Nacl /L.

2) Standard Toxicant: Sodium Chloride (Nacl crystalline)

3) Dilution Water Used: "9:l" Moderately hard reconstituted water

4) Statistics: ICu - calculated by ToxCalc version 5.0 statistical software using EPA-recommended methods O

REFERENCES

1) NPDES Permit No. TN0026450.

2) Lewis, P. A., D. J. Klemm, J. M. Lazorchak, T. J. Norberg-King, W. H. Peltier, M. A. Heber.

Short-Term Methods for Estimatine the Chronic Toxicity ofEffluents and Receivine Waters j

to Freshwater Oreanisrns, EPA /600/4-91/002 (July 1994).

3) Standard Oneratine Procedures. Laboratory Division. Biomonitorine Section. Commonwealth Technology, Incorporated.

4) Standard Methods for the Examination of Water and Wastewater,18th Edition, American Public Health Association.

5) Methods for Chemical Analysis ofWater and Wastes. EPA /600/4-79/020 (March 1983).

f 10

~.

Sequoyah Nuclear Plant Biomoeitoring February 23 - March 2,1999 Appendix B Initial and Final Chemistry for Fathead Minnow 7-day and

),

Daphnid 3-brood Chronic Tests l

l l

O...

Table B-1. Analysis of Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Total Residual Chlorine *, February 22-28,1999 f

1999 Total Residual Month Date Sample Type Chlorine (mg/L)

February 22 Grab

<0.001 February 23 Grab 0.005 February 24 Grab 0.009 February 25 Grab 0.012 February 26 Grab 0.008 February 27 Grab

<0.001 February 28 Grab

<0.001

Sodium hypochlorite is utilized in the essential raw cooling water and in the raw cooling water systems at SQN to control the growth of microbiologically induced bacteria and Asiatic Clams. The treatment is necessary for adequate cooling water flow, efficient heat exchange and corrosion prevention. The

)

injected chemical is diluted by the condenser cooling water flow as well as j

from the flow from other sources.

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i FATHEAD MINNOW commonwealth Technology,Inc.

j LARVAL SURVIVAL AND GROWTH TEST 2520 Regency Road I

Lexington. Kentucky 40503-2921

\\

EPA 600/4-91M02, METHOD 1000.0 (606) 276-3506

] Discharger %n:Tsan95Se Ibl lor. 040er.' bi Datomme Test Terminated:

3-2 ~'I9 IlW &

bbo Islr Mit/Mr Ihnf hll Id/

Organism Batch Number:

02 Bia. Log No:

WO InitialOrganism Age:

(.2't b Datemme Test initiated:

S 13-4 8)

(p 00AA Dilution Water Used: 9! /N

{

Dilution Water Batch Number on Day: 1: 01'1 2: 039 3: 030 4: 0 31 5:015/

6: 0 3 A 7: C 3 2 Time of Day Renewed on Day:

1: II:004A 2: II.'oo M 3: It'eoan.4: 3'IL.J: 9 6 : lotoo 6

w 7: 3 4

No. of Uve Larvae (Days)

Sample 10 Test Tin Wt.of Wt.of Total Average Average Cont.

Sample Dah No.

Tin &

Tin Wt. of Wt. of Wt. for

"- % w w w 24 %

y,~;-

y,~l3 Tl3 "7m1"'

(=a) 1 2

3 4

5 6

7 l

10 10 to 10 lO lO W l

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to 10 10 10 lo 10 10 D

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/0 10 to 10 IO 10 la 3

%o.go 924.46

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10 10 ID 10 10 (0 10 4

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to Ib 10 10 9 8 2 6

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10 10 ID 10 $ 8 l9 99o.71 986*31 4.El o.yr f WM 90 to lD 10 lb S

~4-3-

to qqo.99481. *5G 3.33 0.333 C.352 Analyst AS $R /43,$4 ($ HF Nfu M Mtl

/M3 l'l3 l'lB Notes:

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FATHEAD MINNOW commonwealth Techr: ology, Inc.

LARVAL SURVIVAL AND GROWTH TEST Lexing$n?K ntuchos-2921

~*

EPA 60W4 91A302, METHOD 1000.0 (606) 276-3506

', Discharger /Locaten: Tp,wfier vallc /Aciler:0 i

Dat. Time Test T.rminated:

5-2-44 li:ao A hI(6Lo4 MWlrer IIM don /[ /dl Organism Batch Number:

@#2 Bio. l.og No:

N30 Initia! Organism Age:

(2'f h Datamme Test initiated:

1.~.23-9T l l'. 4 o h Dilution Water Used: i' / S tv.

Dilution Water Batch Number on Day: 1: 029 2: Coi 3: 630 4: 031 5:63/

6: 032-7: O3&

'nme of Day Renewed on Day:

1: //:oe t A 2: //.'d64/r1 3: /I: vein. 4: 3:16, s: 9 Wo-6: ( 0:o o a : ": s glA No. of Uve Larvae (Days)

Sample ID Test Tin Wt. of Wt.of Total Average Average Cont..

Sample Dates No.

Tin &

Tin Wt.of Wt. of Wt. for YJs /23h /M"k 7pf kg g) mg)

)

1 2

3 4 i$5 6

7 3I l0 10 l0 N lD 9-6 Of 10n.90 lom.M 3. 3 s 6.700 5% M M 33 to lo lo O ID 10 9 23 999.39 994.55 3. p tt 0.394 5823 %

N * *k 03 10 10 10 10 lO IO LO 03 994.94 A92.00 4.24 6.494 i%N1%i#

04 Ib 10 10 (O

10 (O O 04 997.00 99Lzo 5.00

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0.49, !@ M R J'h "**/*

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of IO lo ID 10

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30 lo 10 10 4 9 8 9-30 996.63 923.cl 3. 60 0.369 BWhU$

- % 3(

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Analyst

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&=

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Analysis of Survival I

\\

SON l

Starting Date: 0,2/23/99 St 'vival ( % ) Oata Replicate Treatment 1

2 3

4 5

6 7

8 9

10 Mean OONTROL 1.00 1.00 1.00 1.00 1.00 25 0.90 0.80 0.90 0.40 0.75 39.2 0.90 1.00 1.00 0.60 0.88

$9 1.00 0.90 1.00 0.60 0.88 30 0.40 0.80 0.80 0.70 0.68

.00 0.60 0.90 1.00 1.00 0.88

]k Steel's Many-One Rank Test No. of Critical Treatment Replicates Rank Sum Rank Sum

25 4

10 10 39.2 4

10 14 49 4

10 14 80 4

10 10 100 4

10 14

Values less than Critical Rank Sum significantly less than the control (CONTROL

).

i g

~

%/

f3*

3 Analysis of 7-day Larval Growth Test J

SON Starting Date: 02/23/99 4

1 Analysis of Dry Weight (mg) Data Dunnett's Test No transformation applied before data analysis.

For this set of data, the minimum significant difference is 0.17 r

This represents a 28.95 % reduction' in Dry Weight (ms).

T=

2.29 ALPHA = 0.05 Dry Weight (mg) Data Replicate TREATMENT 1

2 3

4 5

6 7

8 9

10 MEAN S

l 1_f0NTROL O.56 0.59 0.60 0.54 0.57 j

2 39.2 0.36 0.56 0.54 0.25 O.43 3 49 0.44 0.51 0.55 0.29 0.45 4 100 O.32 0.38 0.42 0.50 0.41 Asterisk (*) indicates values significantly less than control (CONTROL).

k Analysis of Variance Source DF Sum 0F Sq.

Mean Sq.

Cale F F( 0.05 )

Among 3

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Total 15 0.000 D*

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FATHEAD MINNOW commonwealth Technology,Inc. LARVAL SURVIVAL AND GROWTH TEST 2520 Regtney Roid Lexington. Kentucky 40503-2921 EPA 600/4-91/002, METHOD 1000.0 (606) 276 3506 ) Discharger / Location:hpf5ae l/ /[er, 04 Mar.'lt e 4 Date/ Time Test Terminated: 3-2 '79 llW Am [ hl.to VC 1 NidlMr ?bnf ll 16 i Organism Batch Number: OW Bio. Log No: MO initialOrganism Age: (.2 *I b Dite/ Time Test initiated: S.13 M (p 00 A A Dilution Water Used: @ / b. Dilution Water Batch Number on Day: 1: OM 2;039 3;030 4: o3I 5:03/ 6: 032 7:O32 Time of Day Renewed on Day: 1://f00AtA 2: //.'oo/kn 3: //'eo An. 4: 3 *. lbE: 9 6: f0foow 7: 0 M No. of Uve Larvae (Days) Sample ID Test Tin W1.of Wt.of Total Average Average Cont. Sample Dates No. Tin & iin Wt.of Wt. of Wt. for { Ik %3 %/ %r %s /w %f Tm"g*)* Tm"g*3 Tg*3 "**7 8 (*S) m 1 2 3 4 5 6 7 l 10 10 to 10 10 10 to I qqo.33 Rg4.H S. go 0.gg O pigg@? 2 to to to 10 IO lo (O D I2n c,e t5 tl.92 s.v. o.s,6 M5i$5? C6nkl 3 10 to to 10 IO (O lo .3 990.go 924.%

b. o y

o. goy MMW 4

10 10 10 10 10 (O 10 4 991. sl 9 81. % S.41 0 54I 0.52I T !O 10 10 10 R 9 9 5 93.6s 9 80.\\9 3. 4b 6 344 MS$ f to ib to 10 Q 8 2 6 999.9 4 926.33 3.bl o.361 85EEN Q ~l to /0 ID /0 9 9 T 7 92 4. 0 s 92t.36 p.39

o. pp9 R$$5f5

? 10 10 10 10 9 S 4 8 9fI. V R8I.03 030 0 079 o.9G7 T-(0 lO 9 Q 9 Cf 9 99].10 983.50 '3. (> O 0.340 ???T$$? Io ID 10 to 10 10 10 to to 990.as 9 84.10 5.ss-o.su SWEAN M* * % ll 10 to in 10 lo 10 IO It 993.92 982.y6 s.41 6.c4( EG9628 t) to lo 9 1 (o C 6 lo 99I.oa 982.50 0 50

0. ara 0.407 12 10 10 10

/0 lo /0 to la 940.94 9 28. @ 4.4 'l

o. 44'I if3EISE 14 to ID to lo lo IO 9 14 999.sr 924.si s.o, a se, 2%%s#

15 to lo 10 no ID lo to ir 996.or 990.M s.s 4 0.gs 4 W?B05 16 10 /0 10 10 6 6 6 16 993.36 9 40.95 191

o..WI

0. '/ 4 9 12 lo ID to (O

9 4 4 n 994.ca 994.0h I 95 0.195 EEEUEN It io /D lo ID lo 9 8 Ir 99r.5o 99l.21 4.7I 0.93( M@@M I 'I 19 to 10 10 (D 10 $ 8 19 99o.71 98k3l 4.El o.yr 1 WWW 90 to l0 to ID 9 T-3- ao 990.7<9981. % 3.33 0.333

0. 2s a Anilyst At$.f)R /$ M b Mf./ HA/ #$h

[q ti /US /#13 /$ Notes: Approved By y/ (v3 ~ apownmesuse

\\ FATHEAD MINNOW commonw:alth Technsingy,Inc. LARVAL SURVIVAL AND GROWTH TEST Lexing?o'n?g n~tuc$ <"os$3 2921 EPA 60W4-91AN22, METHOD 1000.0 (606) 276 3506 ] Discharger /tr-:eEn: I#rt#rJ## N/la, Au1%,r.4 DateTime Test Terminated: 8; d II:aoN Allbbeh b!wlGr EI& buNYl lbi organism Batch Number: WN Bio. [og No: N30 InitialOrganism Age: b4h D,temme Test initiated: .1.7.3 -9 T {t,o A Dilution Water Used: Tl / SI. v Dilution Water Batch Number on Day: 1: 0.29 2: C04 3: 630 4: 03f s:6:3/ 6: 037-7: OSE Time of Day Renewed on Day: 1://:oo#.A 2: //.'ocA/n 3:II:ve4A 4: 3;16,5: 9 I 46: (Obo,,y:4'm pjlp No. of Uve Larvae (Days) Sample ID Test Tin Wt.of Wt. of Total Average Average Cont. Sample Dates No. Tin & Tin Wt. of Wt. of Wt. for "' % % % %% % 'ff 7,",*)* y,",*)* Ym"g")***f"gI" { (*S) 1 2 3 4 i$$ s 7 of 10 la to D l0 4 6 at toog.go Iox +o 3.0 s s..yce gggggy D3 /0 10 /0 O 10 IO 9 22 99F.34 994.55 3. ett o.3&9 IS$$i@ Mo% i 03 10 ID to /0 lo IO LO c3 99(,.c4 992.00 4.09 c.Y34 $N?VENI ) lb to 10 (O ID (O O 34 997.99 99,2.20 5.00 650a 6.'l08 or 10 /D IO /0 10 to to or la6a.33 list 16 5.17 6.ri7 $@@n i DE 10 ID 10 10 l0 (O 10 06 12 4t. b 1 IM3 80 4.99 0.'19 1 E#h M ? ** W ** Y* J. og io ID 10 lo lo lO 10 n 10 %.3 o %5,9 4.43 0 443 1%5M Dr 10 lo 10 10 Q 't .3 at 10 %. 0 o f.%H 09 f. 9( ..?. I91 0.4IO 09-Io 10 10 10 (o S 4 39 10 % 89 l245.99 0.09 & YEYMM 30 10 10 to 4 9 8 3-30 976.63 923.cl 3. 60

0. M 9 6MM0$

l4% '**% 3i to 10 /0 9 9 7 si Iort.or in523 3. se 0 3sr UMa& 3a lo 10 lo to Io JP P 3a 9 89.93 @5.% 4.o ; o.4o 7 o.3 4I MWff NDIbk!. WEB %?5 N@!$$$ i Wi@P2 W%tM Anityst /Q /jh f'3 Q l3 pp/ $@ g [1p A)g //jg f;f} f Notes: Approved By Q qkJ o-- h.w ,,. 3 n,-* r ee- - w ..wei. .a= %

w..

.<m-+ . w we r* 4 ema. = = s. m*- - * =. _ eue .gue 4

C# g.<i o1 s Analysis of Survival ~ SON Starting Date: 02/23/99 Survival (% ) Data l Replicate Treatment 1 2-3 4 5 6 7 8 9 10 Mean . ONTROL 1.00 1.00 1.00 1.00 1.00 25 0.90 0.PO O.90 0.40 0.75 39.2-0.90 1.00 1.00.0.60 0.88 . 49- ' 1.00 0.90 1.00 0.60 0.88 30 0.40 0.80 0.80 0.70 0.68 '00 0.60 0.90 1.00 1.00 0.88 J ______'____________________ Steel s Many-One Rank Test No. of Critical Traatment Replicates Rank Sum Rank Sum

25 4

10 10 39.2 4 10 14 49 4 10 14 80 4 10 10 100 4 10 14

Values less than Critical Rank Sum significantly less than the control (CONTROL

). I pa a_ c.- ..-.-,.-..W.~ ~+w..~ - ~ - ~ ~ '

^ ' *

"~"

f(10 } Analysis of 7-day Larval Growth Test SQN Starting Date: 02/23/99 Analysis of Dry Weight (mg) Data Dunnett's Test No transformation applied before data analysis. For this set of data, the minimum significant difference is 0.17 This represents a 28.95 % reduction in Dry Weight (ms). T= 2.29 ALPHA = 0.05 Dry Weight (mg) Data Replicate TREATMENT 1 2 3 4 5 6 7 8 9 10 MEAN S 1_h0NTROL 0.56 0.58 0.60 0.54 0.57 2 39.2 0.36 0.56 0.54 0.25 0.43 3 49 0.44 0.51 0.55 0.29 O.45 4 100 O.32 0.38 0.42 0.50 0.41 Asterisk ( * ) indicates values significantly less than control (CONTROL). i Analysis of Variance Source DF Sum 0F Sq. Mean Sq. Cale F F( 0.05 ) Among 3 0.065 0.022 2.090 0.1545 Within 12 0.125 L--------------------------0.010 Total 15 0.000 1 0 D* ...e s.~ g see - e

COMMONWEALTH TECHNOLOGY,INC. Laboratory Division /^ Larval Fish Survival and Growth Test-Survival Start Date: 2/23/99 11:00 TestID: 7830-1 Sample ID: TV TVA End Date: 3r2/9911:00 Lat ID: CTI-Toxicology Laboratory Sample Type: EFF3-Power Plant Sample Date: 2/22/99 Protocol: EF EPA /600/4-91/002 Test Species: PP-Pimephales promelas Comments: Sequoyah Nuclear Plant / Intake Conc-% 1 2 3 4 Control 1.0000 1.0000 1.0000 1.0000 100 1.0000 1.0000 1.0000 0.3000 Transform: Arcsin Square Root Rank 1 Talled Cone-% Mean N-Mean Mean Min Max CV% N Sum Critical Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 100 0.8250 0.8250 1.2039 0.5796 1.4120 34.569 4 16.00 11.00 Auxiliary Tests Statistic Critical Skew Kurt g spiro-Wilk's Test indcates non-normal distnbuton (p <= 0.01) 0.7064 0.749 -2.0367 4.9 Equality of variance cannot be confirmed HypaGsis Test (1-tall. 0.05) Wilcoxon Two-Sample Test indicates no significant differences l ~.'. O l Page 1 ToxCale v5.0 Reviewed by: 1

COMMONWEALTH TECHNOLOGY, INC. Laboratory Division l ^ Larval Fish Survival and Growth Test Start Growth i I Start Data: 2/23/99 11:00 Test ID: 7830-l Sample ID: TV TVA l End Date: 3/2/9911:00 labid: CTI Toxicologylaboratory Sample Type: EFF3-Power Plant Sample Date: 2/22/99 Protocol: EF EPA /600/4-91/002 Test Species: PP-Pimephales promelas Comments: Sequoyah Nuclear Plant / Intake Conc % 1 2 3 4 Control 0.5620 0.5760 0.6040 0.5410 100 0.5170 0.4870 0.4430 0.1910 i 1 Transform: Untransformed 1 Tailed Cone-% Mean Ahn Mean Min Max CV% N t-Stat Critical MSD Control 0.5708 7.0000 0.5708 0.5410 0.6040 4.630 4

100 0.4095 0.7175 0.4095 0.1910 0.5170 36.338 4

2.134 1.943 0.0111 i _ Auxiliary Tests Statistic Critical Skew Kurt

jhapiro-Wilk's Test indicates normal distr lbution (p > 0.01) 0.84176 0.749

-1.7064 3.87427 j F Test indcates equal variances (p = 0 02) 31.7112 47.4683 Hypothesis Test (1 tail,0.05) Homoscedaste t Test indicates significant difft.rences l i

g Page1 TexCale v5.0 Reviewed by

l COMMONWEALTH TECHNOLOGY, INC. Laboratory Division -N Larval Fish Survival and Crowth Test-Survival Start Date: 2/23/99 11:00 Test ID: 7630-U Sample ID: TV TVA End Date: 3/2/99 11:00 Lab ID: CTI Toxicology Laboratory Sample Type: EFF3 Power Plant Sample Date: 2/22/99 Protocol: EF EPA /600/4-91/002 Test Species: PP-Pimephales prometas Comments: Sequoyah Nuclear Plant / Upstream Cone-% 1 2 3 4 I Control 1.0000 1.0000 1.0000 1.0000 I 100 0.4000 0.7000 0.7000 0.8000 } a Transform: Arcsin Square Root 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4

100 0.6500 0.6500 0.9435 0.6847 1.1071 19.184 4

5.176 2.353 0.0193 [ Auxiliary Tests Statistic Critical Skew Kurt hapiro-Wilk's Test indicates normal distnbution (p > 0.01) 0.79204 0.749 1.4589 4.16768 quility of variance cannot be confirmed l HypGtMs Test (1-tail,0.05) Hittroscedastic t Test indicates significant differences ) i l We m. Page 1 TexCalc v5.0 Reviewed by:

COMMONWEALTH TECHNOLOGY,INC. Laborotovy Division Larval F;sh Survival and Growth Test Start Growth Start Date: 2/23/99 11:00 TestID: 7830-U Sample ID: TV-TVA End Date: 3/2/99 11:00 labid: CTI-Toxicology Laboratory Sample Type: EFF3-Power Plant Sample Date: 2/22199 Protocol: EF-EPA /600/4-91/002 Test Species: PP-Pimephales prometas Comments: Sequoyah Nuclear Plant / Upstream Cone % 1 2 3 4 _ _ ' ~ Control 0.5620 0.5760 0.6040 0.5410 100 0.2090 0.3620 0.3850 0.4070 Transform: Untransformed 1-Talled Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control 0.5708 1.0000 0.5708 0.5410 0.6040 4.630 4

100 0.3408 0.5970 0.3408 02090 0.4070 26.334 4

4.917 1.943 -0.0000 - Statistic Critical Skew Kurt } ~ Auxiliary Tests Shapiro-Wilk's Test indicates normal distnbution (p > 0.01) 0.87388 0.749 -1.5675 3.06231 F-Test indicates equal variances fp = 0.07) 11.532 47.4683 ___ Mais Test (14sil,0.05) Hy Homoscodastic t Test indcates signifcant differences 5 ~ Pagei TerCale v5.0 Fleviewed by;. -~r

Ceriodaphnia dubla SURVIVAL AND commonwealth TechnDiogy,Inc. REPRODUCTION TESTDATA SHEET w ;ngo'n *g"nTe"y a"os*os.2gg, d EPA 600/4-91/002, M:thod 1002.0 (606) 276-3506 Discharger TPsh/MPf Y0llA A$dt:'lt Datemene Test Inttiated: L*lW 4 ' 00 c.w. i l.ocation: M4f/dMA Math /bd ddc/l /d/ Datemrne Test Terminated: } 2 94 R08 'n _, ~) Bio No.: M29 Dilution Water Used: k! / E L. Datomrne initial Sarrple Collected: M-'t 4 04W source culture Date: ONS Nurnbor of Uve Young For Each Test Day (X denotes dead adub) Sarnple Date or Dilution Water Batch Nurnbur [' [' nc Cont nw og og 2g om o3o o 21 OS} (& o% %"e*p.'*'Y*C,*e'* Y 1 2 3 4 5 6 7' 8' O o o lp O 10 IS 34 kR@f 2 o o o q' o ,0

<1

.N e 3 O O O 5 O 19-15 39 o^ o 0 in O 13 llo 35 m lonk*I s O o o lo Q 11 Ig 35 h e O o 0-Lo il o 14 .31 \\ 7 Q Q () (y [Q Q lf }[, 8 (-) 0 Q {g l} l(9 3Q e O o o lo O e In 34 ~ ,o o o o no O lo I5 31 3% No.of Uve Adults l /O l lo l to 10 101/OlloI l g%* Nurnber of Uvs Young.5r Each Test Day '*'**d* "I Emuent Test Co Container Sample Date or Ollution Wjggyatch Number ,f"[' ,g[* 2~M 2.l.3 2-2.' Q 3f M ol[p 2/y{c You Per Yo g Per 1 2 E' 4 5 6 7 8 si O o o O Il o 16 33 W" 12 O o O (p 8 la-I8 3/n 13 m o 0 (o (9 13 15 34 0 C> n (o O 10 Do B lo M ,s O O o (o O Il 18 3% 16 () 0 0 (O O /3 98 L. 17 c) 0 0 (o ll O /(o .33 ~.' s 's n 0 0 (o O to 17 33 ,e o O o (o ID 0 14 3D

== o C o 5' O /> /4 3) 3m 2o l[g /o l (0 '0 110 110 ll6 i ME:EnSE No. of Uve Adults Aa='r52 MR Ju) l N. L6 l LSJ uJl Ls (S -. (S O Am=dBv:y/

If necessary 1nsmnumme

Ceriodaphnia dubla SURVIVAL AND CDmmOnwealth TechnDIDgy, Inc. REPRODUCTION TEST DATA SHEET t,;ngten[g j le y <os$3 2g2, EPA 600/4-91/002, Method 1002.0 (606) 276 3506 Discharger:khtttfpr Y0llot ku$$er;$L Dalemene Test inhiated: 1~23 A'l Y~~ Location: %ffAM MuS, N%f 'Jit M // /d / Datomme Test Termnated: _3-7-99 380.~. 9 Bio No.: M 2.9 Dilution Water Used: S / N. Dab / Time inhial Sarrple Collected: 2 1') D'lM Sourco Culture Date: 02A 5' Nurnber of Uve Young For Each Test Day (X denotes dead adu!!) Efiluent Test Total No. Total No. Conc. Container Sample Date or Dilution pgeg Batch Number og gy, og gy, N-2 22 .2 -2.5 2,2>f 31s-gt, a/g 9/27

"r;g Per Young Per g

1 2 3 4 5 6 7 8 2s o o o g q o /5 SQ M @@yiB 22 O o o y 0 40 th .3 a jf 23 /> o n '7 0 la Ho Bs WEM8 24 O o b 3 O (0 13 2(3 '~ M2 2s o o o '7 0 .5 9 01 1 2s o, o n 6 la o 19 .34 27 (~) Q D (g fQ 0 l$ }l 28 o o o (o C lo 15 31 $qRR$ 2e O O O lo (( O II.o 33 i62 % % D o o (o O 9 I4 29 JN No.of Uve Adults IO l 10 l l0 10 to lol lo i l O Nurnber of Uve Young For Each Test Day (X denotes dead edult) Effluent Test Total No. Total No. Conc. Container Sample Date or Dilution Wge@tch Number og gy, og gy, l-u 2-U 2r 2'i 3-aS" %fu %) 9/2%

" " * ' ' ' ' ""*l*'

ep. 1 2 3 4 '5 6 7 8 31 o o o a o F 13 93

- - - 1 l

32 O o o cl O lO 17 34 W 33 O o a lo I2 O lin 34 fj' 3< o o a 5 / D. O IR 35 ~~ Y ~ ll O 15 3 9, { h ss O n o (o 36 O O O (p [d O l6 33 k$$jl@j{ 37 O O o 5 // O I /7 KWWA5 38 O o o r7 O ll /7 35

am +*

S h; se O o o 6 IO o l'{ 29 e n 0 0 in In O l.S 29 Sol /O l/o lIo llc jo 10 l l0 l MMM@nids No. of Uve Adults Anavsi W Jw I # l L9l ks lJuJl to I 3 LS u Q Approved BF p Corrrnents: evemniescas

Ceriodaphnla dubla SURVIVAL AND CDmmDnw a echnD ncy noajDy, Inc. \\ REPRODUCTION TESTDATA SHEET tsxington. Kzntucky 40s03-2921 22 EPA 600/4-91/002, M:thod 1002.0 (606) 276-3s06 Discharger l0Ahel5W Y0lffl' NloAN Datemme Test tnttiated: I~E Y'GD w. Location: ff///SLIA kttbr Ilde'l. dYf4 // /8 f Datomrns Test Term Dilution Water Used:' gated _3-2-99 3 *

r. i.

] Bio No.: M29 T.' / f L. Datomme initial Sarnple Collected: Z-2.2 't't 0948 Source Culture Date: 6,2O} Nurrter of Uve Young For Each Test Day (X denotes dead ailt) Effluent Test Conc. Container Sample Date or Dilution gtjatch Number Tntal No. Total No. N g i 9, og gy, 1-21 2-23 w o.or.h Blan alge Y*"",0, F Y*""a P*' 1 2 3 4 s 6 7 8 <1 O o o 5 (O 'O (6 31 %#*'W 42 o e o a o il l'7 3n 2 <3 n o o to o Ill 30 l O O o 9 0 II /Lo 3A f 90 4s o o o (n ll 0 (q 34 's n O o Lj ll O [4 y g 47 O O o lo er O 15 29 48 o O o (o o I. /R 36

9 O

O o lo IO O IS S\\ i so () O' o Q; O (, g 90 3ba No. of Uve Adufts f() l lD l {d jQ fo l 101 /01 l Number of Uve Young For Each Test Day (X denotes dead sitt) Effluent Test Conc. ' Container Sample Date or Dilution Wptgatch Number Total No. Total No. N og gy, or gy, .2.12 2-n 21q o.of 2/g afaq p/zg Yo g Per Yo ng Per 1 2 3 4 s 6 7 8 si O o 6 /, IO O /6 3'a k s2 O O o (o O 4 I5 3D d 53 O O o n 0 0 'O 6* $n$$ 54 o o o (o O 9 M cp9 ~ ~ IDb ss o o o ry q o l(o 39 ss O o n c/ q o /c2. o78 57 o e) 0 6 0 10 14 3D j?4W s8 0 o a r7 ll O jy' 39 $' ~ kBht ss U o o r7 ID D / C, .3 3 @AN5 O o o i5 o lo is-30 db No. of Uve Adults {lg lg ' 10 l /O \\ /O \\ lO \\ }O hdAWESd5SE Anest IhU l Juh ABl[S LS LM l L5 l J 3. O commona *cun H3 e ew~d ca Aksi h c 2=L. I n

Ceriodaphnia dubia SURVIVAL AND commonwcalth TcChnology, Inc. REPRODUCTION TEST DATA SHEET a x;ng o'n *$ h*l f os# aa.292, (606) 276-3506 Discharger:Trasorror Volkr. #<ste,4v Dais / rime Test initiated: ewM W o.m. Location: bl/6tc4 M&rle, # //dd 6/Ja// /o/ Date/ Time Test Terminated: 3-2 'lO 3 ** n ) Blo No.: N2R D0ution Water Used: 9 ' / E. L-Date/ Time initial Sample Collected: .1 -22.- 'I T OH8 Source Culture Date: 820F Number of Uve Young For Each Test Day (X denotes dead adult) Effluent Test Total No. Total No. Conc. Container Sample Date or Dilution Bpitppatch Number of Uve of Uve (%) No. f.y y, g Q a Young Per Yo g Per 1 2 3 4 5 6 7* 8' lll O o '7 O li l Lo 3H (1 8 n a O n o (n O l0 15 31 yk R O n n o (p O \\\\ 15 39 W: n O n o 'T O lo l(> 33 : MHoo tr O O O G O Il Do 31 i K n o o r1 lo o l5 33 BBN@ 67 O o o (o '7 3 /0 h "~$$ ~ br O O o le 5 ll I'l %lo M O o o n O lo lls 33 EM Jo o o 6 (o O 2 11 23 3lQ No.of Uve Adults fU lQ /d lC lQ lQ /C l Number of Uve Young For Each Test Day (X denotes dead adult) Effluent ' Test Total No. Total No. Conc. Container Sample Date or Dilution Wa.te4 atch Number of yy, of uve j B (%) No. 3, g Q Young Per Yo er 1 2 3 4 '5 6 7 8 ~1/ O o 0 '7 12 8 17 3'7 ! 3munat 7.2 O O o r1 0 D- /5 3' 73 o o o '1 O Jl / 6' 33 pumang ,4 0 o o '7 0 1 I is 33 m l k k -'*o 2.r O O O (o II O I(o R3 =[Q 11 0 0 0 G IO O I4 3D i 47 n O o (o 9 O 15 3D ff$i? 76 O O O '1 O lO ILe 3S ??W 99 O O U (s D ll lE B5 $$ p o o o 9 3 8 l'7 J35.33a No of Uve Adults 10 ./ o 'O 10 lo lo, Io ti&%Js=?" ^ ^ * ' ' "# "") s Ls ts aus Le is -.. Ls O Approved By:

If necessary em,vam,ssc.se
3.._,-.,...._.

I i j ) THE NUMBER OF RESAMPLES IS. 80 So Fib. 21, l T T i t i Cee Te GA 03-W" n }** LISTING OF GROUP CONCENTRATIONS ( % EFF. ) AND RESPONSE MEANS *** .+ CONC. ( %EFF ) RESPONSE MEAN MEAN AFTER POOLING .000 33.600 33.600 2S.000 32.900 32.900 39.200 29.900 30.067 1 ) 49.000 30.100 30.067 80.000 30.200 30.067 100.000 27.600 27.600 coo NO LINEAR INTERPOLATION ESTIMATE CAN BE CALCULATED FROM THE INPUT DATA, SINCE NONE OF THE (POSSIBLY POOLED) GROUP RESPONSE MEANS WERE LESS THAN 75.0% OF THE CONTROL RESPONSE MEAN. DATA. SINCE NONE OF THE (POSSIBLY POOLED) GROUP RESPONSE MEANS WERE LESS THAN 75.0% OF.THE CONTROL RESPONSE MEAN. O BOOTSTRAP PROCEDURE TO ESTIMATE VARIABILITY OF THE ESTIMATE

- - - - D ICp THE MEAN OF THE BOOTSTRAP ESTIMATES IS 95.7876.

THE STANDARD DEVIATION OF THE BOOTSTRAP ESTIMATES IS 3.9758. t AN EMPIRICAL ****% CONFIDENCE INTERVAL FOR THE BOOTSTRAP ESTIMATE IS ( 8S.9347, 99.7368). con NOTE: THE ABOVE BOOTSTRAP CALCULATIONS WERE BASED ON 1S INSTEAD OF 80 RESAMPLINGS. THOSE RESAMPLES NOT USED HAD ESTIMATES ABOVE THE HIGHEST CONCENTRATION / % EFF.

\\BOOTSTRP)

COMMONWEALTH TECHNOLOGY, INC. Laboratory Division ' \\ Daphnid Survival and Reproduction Test-Survival Start Date: 2/23/99 11:00 Test ID: 78291 Sample ID: TV TVA End Date: 3/2/99 11:00 Lab ID: CTI Toxicology Laboratory Sample Type: EFF3-Power Plant Sample Date: 2/22/99 Protocol: EF-EPN600/4-91/002 Test Species: CD-Ceriodaphnia dubia Comments: Sequoyah Nuclear Plant / Intake Cono % 1 2 3 4 5 6 7 8 9 10 Control 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 100 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Not Fishers 1-Tailed Conc-% Mean N-Mean Resp Resp Total N Exact P Critical Control 1.0000 1.0000 0 10 10 10 100 1.0000 1.0000 0 10 10 10 1.0000 0.0500 ,,,4ypa; Mis Test (1-tail. 0.05) NOEC LOEC ChV TU Qshers Exact Test >100 >100 <, 1 t' O Page 1 TexCalc v5.0 Reviewed by: m.w n ~

COMMONWEALTH TECHNOLOGY, INC. Laboratory Division ' 'N Daphnid Survival and Reproduction Test-Reproduction ' Start Date: 2/23/99 11:00 Test ID: 7829-l Sample 10: TV 1VA End Date: 3/2/99 11:00 Lab ID: CTI-Toxicology Laboratory Sample Type: EFF3-Power Plant Sunple Date: 2/22/99 Protocol: EE-EPN600/4-91/002 Test Species: CD-Ceriodaphnia dubia Comments: Sequoyah Nuclear Plant / Intake Conc-% 1 2 3 4 5 6 7 8 9 10 Control 34.000 34.000 32.000 35.000 35.000 31.000 36.000 34.000 34.000 31.000 100 34.000 31.000 32.000 33.000 37.000 32.000 26.000 36.000 33.000 25.000 Transform: Untrs.nsformed 1-Tailed Conc-% Mean N-Maan Mean Min Max CV% N t-Stat Critical MSD Control 33.600 1.0000 33.600 31.000 36.000 5.097 10 100 31.900 0.9494 31.900 25.000 37.000 12.046 10 1.278 1.734 3.069 uxiliary Tests Statistic Critical Skew Kurt iro-Wilk's Test indicates normal distnbution (p > 0.01) 0.92506 0.868 -0.8209 1.1875 .Tsst indicates equal variances (p = 0.02) 5.03409 6.54109 Hypothesis Test (1-tall,0.05) Homoscedastic t Test indicates no significant differences i ee a Page 1 TexCale v5.0 Reviewed by: _. _._

COMMONWEALTH TECHNOLOGY, INC. Laboratory Division ^ Daphnid Survival and Reproduction Test Survival Start Date: 2/23/99 11:00 Test ID: 7829-U Sample ID: TV TVA End Date: 3/2/99 11:00 labid: CTI Toxicology Laboratory Sample Type: EFF3-Power Plant Simple Date: 2/22/99 Protocol: EF EPA /600/4-91/002 Test Species: CD-Ceriodaphnia dubia Comments: Seouoyah Nuclear Plant / Upstream Conc-% 1 2 3 4 5 6 7 8 9 10 Control 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 100 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Not Fisher's 1-Tailed Conc-% Mean N-Mean Resp Resp Total N Exact P Critical Control 1.0000 1.0000 0 10 10 10 100 1.0000 1.0000 0 10 10 10 1.0000 0.0500 Hypothesis Test (1-tall. 0.05) NOEC LOEC ChV TU .pher's Exact Test 7100 >100 <. 1 4 Page1 TexCale v5.0 Reviewed by: ~ -en ..~.

COMMONWEALTH TECHNOLOGY, INC. Laboratory Division c ~ T Daphnid Survival and Reproduction Test-Reproduction Start Date: 2/23/99 11:00 Test ID: 7829-U Sample ID: TV TVA End Date: 3/2/99 11:00 labid: CTI Toxicology Laboratory Sample Type: EFF3-Power Plant Sample Date: 2/22/99 Protocol: EF EPA /600/4-91/002 Test Species: CD-Ceriodaphnia dubia Comments: Sequoyah Nuclear Plant / Upstream Cone-% 1 2 3 4 5 6 7 8 9 10 Control 34.000 34.000 32.000 35.000 35.000 31.000 36.000 34.000 34.000 31.000 100 37.000 34.000 32.000 33.000 33.000 30.000 30.000 33.000 35.000 35.000 l Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control 33.600 1.0000 33.600 31.000 36.000 5.097 10 100 33.200 0.9881 33.200 30.000 37.000 6.630 10 0.454 1.734 1.349 Auxiliary Tests Statistic Critical Skew Kurt h7apiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95586 0.868 0.1647 -0.4491 F-Tist indicates equal variances (p = 0.47) 1.65152 6.54109 My,,,,t;.e.;s Test (1-tail. 0 05) Homoscadastic t Test indicates no significant differences i O Pa9e1 ToxCale v5.0 Reviewed by:

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SHORT-TERM CHRONIC TOXICITY TEST commonw:alth Technology, Inc. 5 l TEMPERATURE RECORD g,,,,g,,,fyjuy4og%.292, (606) 276-3506 Discharge:Tremnn Ya/b A&kr:b Test initiation Date / Time: 2-2399 11:co m Location: MLatd Fus;f Pluf, o#f// tol Test Protocol Temperature: Of. O t 1*C Bio Log No.: ' '4430 Test Organism: C. dubia,@romelap 1 (circle one) M. beryllina, M. Dania Other iSAniple. ?. Temperature ('C)):- l identification" Day 0; fDay[1: / Day.'2[ f Day 3) iD'ay 4f s Day 5:. ! Day l6! ! Dhyl79 ). Day 8.. Coalre { I-T. D $4.Q JN.G Q,[ l }[, g ffb d 9,0 0 0.] Of /o N' 6 247 52.V.9 2E3 GE. l J5l X/ M8 "$?.08g OV.5' % I. ] %0 353 AT.0 @,2 .2 9./ Z48 99#4 MV-[f.049 M. 6 D r. 3 NiO O(9 .25.1
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SHORT-TERM CHRONIC TOXICITY TEST commonwealth Tcchnology, Inc.
S R'9*"* R**d TEMPERATURE RECORD
/ Lexington, Kentucky 40503-2921 i (606) 276-3506 Discharge:7 A c Crpf lb[k, 4 der:L Test Initiation Date / Time: l'J3 0
op,w, Location: Srotawh /Voelyle rh.4 64M// /o/
Test Protocol Temperature: 'JS. O i 1*C Bio Log No.: M29 Test Organism: (C.~ dub 7a7f. promelas (circle one) M. beryllina, M. bahia Other , {SAr6dl6h - A E 'px N (Temperstdre ('C)i 4 ildentificgtj i Fedecl ZS;o 2A. 0 26 Wf $4.1,949 M.3 e4.'2 I 2r% .14. C 44.I Me 34.7 D 24.3 I,.241 .2 4.r J)4.3 29.D% 2't. 5 M. I or.o ar.t M.lo 250 24.2 S4.1 W/n 24.f 293 04.9 'a r.3,D % c950 as'.o Jil.9 86 % MO .24 3 f.o o r. 1 QQ,q Q$,'} a 5.1 Y.R IW/o $. I A4*3 2To Dr. O QQ S' cQ5~5 .?T.o 06.D T6fe - IAr% 6, i 24 5 M.1 M.T W.lo SS1 24.2 Dl. '] Ur, w. ion, '39 2.45 M8 nc, DH.lo &5.a a4.7 94R O Analyst Stj hF J' M 3 6 M MMmm mm Comments: 71b (%p) = 0 V.7 ca4.o-as 3) O .~. ~
SON 0299 February 22.1999 INITIAL AND FINAL WATER CEMISTRY (FATHEAD) Initial Chemistry i Fish - Control 0 1 2 3 4 5 6 MIN MAX MEAN ~g j Temp 25.0 25.0 25.0 25.0 DO 7.6 7.7 8.0 7.7 7.8 7.6 7.6 7.6 8.0 7.7 pH 7.8 8.0 7.7 8.0 7.5 7.8 7.8 7.5 8.0 7.8 Cond 348 354 348 368 345 369 369 345 369 357 Hard 102.0 102.0 101.0 102.0 102.0 108.0 108.0 101.0 108.0 103.6 Alk 90.0 90.0 81.0 89.0 89.0 87.0 87.0 81.0 90.0 87.6 Concentration 25% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.6 24.6 24.6 26.6 DO 7.9 8.0 8.0 7.8 7.7 7.6 7.8 7.6 8.0 7.3 pH 7.8 7.5 7.6 7.6 7.3 7.4 7.6 7.3 7.8 7.5 _ Cond 311 307 300 296 304 314 316 296 316 307 i Concentration 39.2% 0 1 2 3 4 5 6 MIN. MAX MEAN Temp 24.5 24.5 24.5 24.5 DO 7.9 8.0 8.1 7.9 7.8 7.8 7.9 7.8 8.1 7.9 pH 7.8 7.4 7.6 7.5 7.3 7.4 7.5 7.3 7.8 7.5 Cond 285 281 277 2 73 275 286 289 273 289 281 } Concentration 49% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.8 24.8 24.8 24.8 DO 8.0 8.0 8.1 8.0 7.9 7.9 7.9 7.9 8.1 8.0 pH 7.8 7.4 7.5 7.4 7.3 7.4 7.5 7.3 7.8 7.5 Cond 259 261 259 255 256 26a 269 255 269 261 Concentration 80% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.0 25.0 25.0 25.0 DO 8.1 8.0 8.2 7.9 7.9 8.0 7.9 7.9 8.2 8.0 pH 7.6 7.3 7.4 7.3 7.2 7.3 7.3 7.2 7.6 7.3 Cond 205 200 203 202 195 209 210 195 210 203 Concentration 100% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.1 25.1 25.1 25.1 DO 8.2 8.0 8.3 8.1 8.0 8.2 8.2 8.0 8.3 8.1 pH 7.5 7.1 7.3 7.2 7.1 7.2 7.2 7.1 7.5 7.2 Cond 167 161 165 165 158 170 169 158 170 165 Hard 57.0 55.0 59.0 60.0 60.0 65.0 58.0 55.0 65.0 59.1 Alk 54.0 54.0 58.0 58.0 58.0 58.0 59.0 54.0 59.0 57.0 TRC <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 Page1of3
Intake 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.1 25.1 25.1 25.1 DO 8.1 8.0 8.2 8.0 7.8 83 8.3 7.8 8.3 8.1 pH 7.4 7.1 ' 7.2 7.2 7.1 7.2 7.2 7.1 7.4 7.2 Cond 164 156 181 169 150 1 73 166 150 181 166 Hard 56.0 53.0 57.0 59.0 59.0 60.0 58.0 53.0 60.0 57.4 Alk 52.0 55.0 58.0 59.0 59.0 58.0 59.0 52.0 $9.0 57.1 TRC <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 + Upstream 0 1-2 3 4 5 6 MIN MAX MEAN Temp 24.9 24.9 24.9 24.9 DO 8.1 8.3 8.3 8.1 7.8 8.3 8.3 7.8 8.3 8.2 pH 7.5 7.1 7.1 7.2 7.2 7.1 7.1 7.1 7.5 7.2 Cond 162 154 172 170 147 168 167 147 172 163 Hard 54.0 55.0 58.0 60.0 60.0 57.0 59.0 54.0 60.0 57.6 j Alk 52.0 51.0 55.0 57.0 57.0 56.0 59.0 51.0 59.0 55.3 i TRC <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 Final Chemistry FISH i Fish - Medium 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.2 24.9 25.1 25.1 25.0 25.0 24.9 24.2 25.1 24.9 DO 5.6 5.6 6.0 5.7 5.5 5.7 6.2 5.5 6.2 5.8 pH 7.4 7.9 7.4 7.1 7.0 7.1 7.3 7.0 7.9 7.3 Cond 0 0
DIV/0!
Concentration 25% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.7 24.9 25.3 25.1 25.1 25.1 24.8 24.7 l 25.3 25.0 DO 5.1 5.5 5.7 5.6 5.4 5.7 6.2 5.1 6.2 5.6 pH 7.2 7.7 7.2 7.2 6.9 7.2 7.4 6.9 7.7 7.3 Cond 0 0
DIV/0!
Concentration 39.2% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.1 25.0 25.2 25.0 25.2 25.1 24.8 24.8 25.2 25.1 DO 5.0 5.6 5.8 5.2 5.4 5.8 6.5 5.0 6.5 5.6 _, pH 7.2 7.6 7.2 7.2 6.9 7.1 7.4 6.9 7.6 7.2 Cond 0 0
DIV/0!
Concentration 49. I 2 3 4 5 6 7 MDJ MAX MEAN Temp 24.8 24.6 25.2 25.0 24.8 25.1 24.8 24.6 25.2 24.9 DO 5.7 5.3 5.7 5.2 5.1 5.8 6.7 5.1 6.7 5.6 pH 7.1 7.5 7.1 7.0 6.9 7.0 7.3 6.9 7.5 7.1 Cond 0 0
DIV/0!
() Page 2 0f 3
Concentration 80% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.8 24.7 25.2 25.0 25.0 25.2 24.7 24.7 25.2 24.9 { DO 5.6 5.1 5.5 5.1 5.2 5.6 6.8 5.1 6.8 5.6 pH 7.0 7.3 7.0 6.9 6.8 6.9 7.2 6.8 7.3 7.0 Cond i 0 0
DIV/0!
i) Concentration 100% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.9 24.7 25.2 24.9 25.1 25.2 24.7 24.7 25.2 25.0 DO 5.2 5.2 5.4 5.3 5.3 5.5 6.4 5.2 6.4 5.5 pH 6.8 7.2 6.9 6.8 6.8 6.8 7.0 6.8 - 7.2 6.9 Cond 0 0
DIV/0!
Intake 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.3 24.8 25.5 24.9 25.1 25.2 24.8 24.3 25.5 24.9 DO 6.2 5.1 5.6 5.3 5.2 5.5 6.1 5.1 6.2 5.6 pH 6.9 7.1 6.9 6.8 6.7 6.8 6.9 6.7 7.1 6.9 Cond 0 0
DIV/0i Upstream 1
2 3 4 5 6 7 MIN. MAX MEAN Temp 24.5 24.8 25.5 25.0 25.2 25.1 24.9 24.5 25.5 25.0 DO 6.3 5.5 5.8 5.3 5.2 ' 5.2 6.0 5.2 6.3 5.6 pH 7.0 7.1 6.9 6.8 6.7 6.8 7.0 6.7 7.1 6.9 Cond 0 0
DIV/0!
] (p Test Temperature Mean 25.0 i i Minimum 24.2 Maximum 25.5 Excel Reviewed by:Jg. 03 3L-U Word Review by. Q.4. 02 3)-H i Page 3 of3 6 '.eM ar.q,
SON 0299 February 22-28.1999 INITIAL AND FINAL WATER CEMISTRY (CERIO) ]) Initial Chemistry CERIO Control 0 1 2 3 4 5 6' MIN MAX MEAN Temp 25.0 25.0 25.0 25.0 DO 7.6 7.7 8.0 7.7 7.8 7.6 7.6 7.6 8.0 7.7 pH 7.8 8.0 7.7 8.0 7.5 7.8 7.8 7.5 8.0 7.8 Cond 348 354 348 368 345 369 369 345 369 357 Hard 102 102 101 102 102 108 108 101.0 108.0 103.6 Alk 90 90 72 89 89 87 87 81.0 90.0 87.6 Concentration 25% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.6 24.6 24.6 24.6 DO 7.9 8.0 8.0 7.8 7.7 7.6 7.8 7.6 8.0 7.8 pH 7.8 7.5 7.6 7.6 7.3 7.4 7.6 7.3 7.8 7.5 Cond 311 307 300 296 304 314 316 296 316 307 Concentration 39.2% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.5 24.5 24.5 24.5 DO 7.9 8.0 8.1 7.9 7.8 7.8 7.9 7.8 8.1 7.9 pH 7.8 7.4 7.6 7.5 7.3 7.4 ' 7.5 7.3 7.8 7.5 Cond 285 281 277 2 73 275 286 289 2 73 289 281 Concentration 49% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.8 DO 8.0 8.0 8.1 8.0 7.9 7.9 7.9 7.9 8.1 8.0 pH 7.8 7.4 7.5 7.4 7.3 7.4 7.5 7.3 7.8 7.5 Cond 259 261 259 255 256 268 269 255 269 261 Concentration 80% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.0 25.0 25.0 25.0 DO. 8.1 8.0 8.2 7.9 7.9 8.0 7.9 7.9 8.2 8.0 pH 7.6 7.3 7.4 7.3 7.2 7.3 7.3 7.2 7.6 7.3 Cond 205 200 203 202 195 209 210 195 210 105.4 O = PaEC 1 of 3 1
Concentration 100% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.1 25.1 25.1 25.1 DO 8.2 8.0 8.3 8.1 8.0 8.2 8.2 8.0 8.3 8.1 g j pH 7.5 7.1 7.3 7.2 7.1 7.2 7.2 7.1 7.5 7.2 ~ Cond 167 161 165 165 158 170 169 158 170 165 l Hard $7.0 $5.0 59.0 60.0 60.0 65.0 58.0 55.0 65.0 59.1 Alk 54.0 54.0 58.0 58.0 58.0 58.0 59.0 54.0 59.0 57.0 j TRC <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 a Intake 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.1 25.1 ' 25.1 25.1 DO 8.1 8.0 8.2 8.0 7.8 8.3 8.3 7.8 8.3 8.1 pH 7.4 7.1 7.2 7.2 7.1 7.2 7.2 7.1 7.4 7.2 Cond 164 156 181 169 150 173 166 150 181 166 Hard 56.0 53.0 57.0 59.0 59.0 60.0 58.0 53.0 60.0 57.4 Alk 52.0 55.0 $8.0 59.0 59.0 58.0 59.0 52.0 59.0 57.1 TRC <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 Upstream 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.9 24.9 24.9 24.9 DO 8.1 8.3 8.3 8.1 7.8 8.3 8.3 7.8 8.3 8.2 pH 7.5 7.1 7.1 7.2 7.2 7.1 7.1 7.1 7.5 7.2 _ Cond 162 154 172 170 147 168 167 147 172 163 Hard 54.0 55.0 58.0 60.0 60.0 57.0 59.0 54.0 60.0 57.6 } Alk 52.0 51.0 55.0 57.0 57.0 56.0 59.0 51.0 59.0 55.3 TRC <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 Final Chemistry CERIO Ceno - Medium 1 2 3 4 5 6 7 MIN MAX MEAN Temp. 24.0 24.6 24.5 24.1 24.8 24.3 24.2 24.0 24.8 24.4 DO 7.4 7.9 7.8 7.5 7.4 7.7 7.7 7.4 7.9 7.6 pH 8.0 7.9 8.1 7.6 7.6 7.6 7.9 7.6 8.1 7.8 Concentration 25% 1 2 3 4 5 6 7 MIN MAX MEAN Temp. 24.1 24.6 24.7 24.3 24.7 24.5 24.3 24.1 24.7 l 24.5 ___ DO 7.6 7.9 7.9 7.6 7.6 7.9 7.7 7.6 7.9 7.7 pH 8.0 7.9 8.0 7.5 7.6 7.6 7.9 7.5 8.0 7.8 Page 2 of 3
Concentration 39.2% 1 .2 3 4 5 6 7 MIN MAX MEAN Temp. 24.1 25.0 25.1 24.6 25.0 24.8 24.7 24.1 25.1 24.8 DO 7.8 7.9 8.0 7.5 7.7 7.9 7.7 7.5 8.0 7.8 ] pH 8.0 7.8 8.0 7.5 7.6 7.6 7.9 7.5 8.0 7.8 Concentration 49% 1 2 3 4 5 6 7~ MIN MAX MEAN Temp. 24.3 24.9 25.3 24.6 25.0 25.0 24.8 24.3 25.3 24.8 DO 7.9 8.0 8.0 7.6 7.7 8.0 7.8 7.6 8.0 7.9 pH 8.0 7.8 8.0 7.5 7.6 7.6 7.8 7.5 8.0 7.8 Concentration 80% 1 2 3 4 5 6 7 MIN MAX MEAN Temp. 24.3 25.0 25.2 24.7 25.2 25.1 24.8 24.3 25.2 24.9 DO 8.0 8.0 8.1 7.6 7.7 7.9 7.8 7.6 8.1 7.9 pH 8.0 7.7 7.9 7.5 7.6 7.5 7.8 7.5 8.0 7.7 Concentration 100% 1 2 3 4 5 6 7 MIN MAX MEAN Temp. 24.3 25.0 25.0 24.8 25.3 25.0 25.0 24.3 25.3 24.9 DO 7.9 7.9 8.1 7.6 7.8 8.0 7.8 7.6 8.1 7.9 pH 8.0 7.7 7.9 7.4 7.5 7.5 7.7 7.4 8.0 7.7 Intake 1 2 3 4 5 6 7 MIN MAX MEAN h Temp. 24.3 24.9 24.8 24.6 25.2 24.8 24.7 24.3 25.2 24.8 DO 8.0 7.9 8.0 7.6 7.8 8.0 7.8 7.6 8.0 7.9 pH 8.0
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7.8 7.4 7.5 7.4 7.6 7.4 8.0 7.6 Upstream 1 2 3 4 5 6 7 MIN MAX MEAN Temp. 24.3 24.8 24.6 24.6 25.2 24.7 24.7 24.3 25.2 24.7 DO 8.0 7.8 8.1 7.7 7.8 8.0 7.8 7.7 8.1 7.9 pH 8.0 7.5 7.8 7.4 7.5 7.4 7.6 7.4 8.0 76 Test Temperature Mean: 24.7 Min: 24.0 Max: 25.3 Excel Reviewed by: Cl>L 63-3 l-T1 Word Review by: QA 0331 'M 0 Page 3 of 3 {
.) Sequoyah Nuclear Plant Biomonitoring i February 23 - March 2,1999 i Appendix D Reference Toxicant Tests and g. Control Chart Information i I
l COMMONWEALTH TECHNOLOGY, INC. Labora tory Division i l . ~~ CHRONIC REFERENCE TOXICANT TEST DATA Pimepha!es promelas 6000-5000 \\ 4000 mi +2SD g-At 'y +1 SD V 3000 N i I / j -2 SD 1000 s5 8_c sF8cs8lsieesc R s - - - o o 8 ao 8 o -o 8 o Test Date i i Dites l Values Mean -1 SD -2 SD +1 SD +2 SD 0'. 0WI 2752.0000 0 08/ii'97 e55.0000 3403.5000 2482.13ee 2560.7797 4324.8e01 5246.2203 09/11/97 3091.0000 3299.3333 2623.3124 1947.2914 3975.3543 4651.3752 10/21/97 3476.0000 3343.5000 2784.5078 2225.5155 3902.4922 4461.4845 11/03/97 2886.0000 3252.0000 2726.4379 2200.8758 3777.5621 4303.1242 12/09/97 4344.0000 3434.0000 2786.1448 2138.2895 4081.8552 4729.7105 01/16/98 3371.0000 3425.0000 2833.1126 2241 2252 4016.8874 4608.7748 02/04/98 2500.0000 3309.3750 2671.2241 2033.0732 3947.5259 4585.6768 03/09/98 3041.0000 3279.5556 2675.9540 2072.3525 3883.1571 4486.7586 04/07/98 2564.0000 3208.0000 2595.5826 1983.1651 3820.4174 4432.8349 05/16/98 2817.0000 3172.4545 2579.6242 1986.7938 37652849 4358.1153 06/15/98 2007.0000 3075.3333 2417.5428 1759.7522 3733.1239 4390.9145 07/15/98 1928.0000 2987.0769 2281.4633 1575.8497 3692.6905 4398.3042 08/03/98 3380.0000 3015.1429 2329.1261 1643.1092 3701.1597 4387.1765 05/20/98 2699.0000 2994.0667 2327.9837 1661.9008 3660.1496 4326.2326 10/05/98 2543.0000 2965.8750 2312.5718 1659.2686 3619.1782 4272.4814 11/04/98 2669.0000 2948.4118 2311.7689 1675.1260 3585.0546 4221.6975 12/08/98 2382.0000 2916.9444 2285.0460 1653.1476 3548.8428 4180.7412 01/06/9p 2199.0000 2879.1579 2243.3583 1607.5587 3514.9575 4150.7571 02/12/99 2d11.0000 2855.7500 2228.1164 1600.4829 3483.3836 4111.0171 0 2
FATHEAD MINNOW ?***"*g*g*l,h[,'fg",*b*W**- LARVAL SURVIVAL AND GROWTH TEST Lex,ngton, ventucky 40503 2921 EPA 600/4-91/002, METHOD 1000.0 (606) 276-3506 Test Type: Reference Toxicant DaterTime Test Terminated: 3-IT T9 /J:08 PA Toxicant: Sodium Chloride Organism Batch Number: O 2./d.- Bio. Log No: 7800 InitialOrganism Age: < 24 hrs Dste/ Time Test initiated: JL//J./91 //:804/*t ~ Dilution Water Used: 9:1 RW - Dilution Water Batch Number on Day: 1: C 23.- 2: 000 3:002 4: 0.zi 5: O M 6: M - 7: OM Time of Day Renewed on Day: 1:b:00 Aa 2: 11:06 % 3: l O 'a2c % :4 90CA.5;/o.oclt..#: IO.c0<.~. No. of Live Larvae (Days) Mean Mean Test Tin Wt. of Wt. of Total Wt. of Wt. of Average Test Cont. Sample Dates No. Tin & Tin Wt. of Larvce Larvae Wt. for Conc. No. Larvae (mg) Larvae L' sing Using Treatment (mg/L) Y/,;L k/J.. NJ-h hf6 O//r 3/5-(mg) (mg) Start End (mg) Number Number 1 2 3 4 5 6 7 (mg) (mg) 1 'lo lo lo 10 Id /0 IO I 9f93( 989./9 f16
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0AQ 5 to /o (0 l0 /0 lb (8 5 496.7 ? 990.09 6.49 0.649 0.544 Start No. 6 IO $0 (O (Q ff {0 IQ (, @ l,32 Qgt.'t o $, g}. 9,5g). b,5%p O. (ll; 000 7 /o lo 10 IO /0 IO Io 7 qqa i, 9P7.39 5.79 0.57g 0.s78 End No. a /0 /0 (0 lo /o (o ' lo P qq7.o7441 IV 5'.93 c.593 0.593 0574 9 10 lo (0 lo /0 10 lo 9 944. II, 9f4.63 4.G 0.453 0.q53 Start No. 10 to lo 10 lo /o (O to lo %7.ot,990.79 4.07 o.4 71 o.427 o 41,8 2000 11 10 lo 10 10 /0 (0 10 ll 9TI.9I 9F3.W' 4.43 0.443 o Q43 End No. 12 /0 Io [0 to /g (O /0 13 4gqaq 993.9 I 5'TR o.648 0.542 0.467 7-E 4 V-4 13 4F7. ol Q&r.N ), o1 0.107 n.Db2 Start No. to lo 1a 14 /0 Io 6 4 4 9 4 l'f 9n.47 9F7.3S ).if 0.11C 0.222 a.118 4000 15 Io la 6 6 5 3 3 ly 472.a9 9F7.(,f 0.bl
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FATHEAD MINNOW Commonwealth Tcchnoingy, Inc. 2520 Regency Road LARVAL SURVIVAL AND GROWTH TEST Lexington. Kentucky 40503-2921 EPA 600/4-911002, METHOD 1000.0 (606) 276-3506 ( '. dest Type: Reference Toxicant DatWTime Test Terminated: 01199 l#!o' I ^- Tcxicant Sodium Chbride Organism Batch Number: 02/A-Bio. Log No: 700 Initial Organism Age: < 24 hrs DitdTime Test initiated: 3-f ID-l49 ll; o d A A Dilution Water Used: 9:1 RW Dilution Water Batch Number on Day: 1: 0.PD-2:030 3: 002 4: off 5: 6: 7: Time of Day Renswed on Day: 1: 10:oc % 2: h:06 M 3: (O.W4: 5: 6: No. of Uve Larvae (Days) Mean Mean Test Tin Wt.of Wt. of Total Wt. of Wt.of Average Test Cont. Sample Dates No. Tin & Tin WL of Larvae Larvae Wt. for Conc. No. Larvae (mg) Larvae Using Using Treatment (mg/L) h/J #f/Nda #flL (mg) (mg) Start End (mg) Number Number 1 2 3 4 5 6 7 (mg) (mg) 21 3 O Start No. 22 8 3 0 10,000 23 4 3 O End No. 24 8 3 O Start No. End No. Start No. End No. Start No. End No. Analyst AS /$ hp $@f Notes: Approved By (Y/ ~ ~ -. ... - ~ ~. -
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COMMONWEALTH TECHNOLOGY,INC. La b o ra t o ry Divisio n Larval Fish Survival and Grov/11 Test-Start Growth Start Date: 2/12/99 12:00 Test ID: 7800 Tample ID; REF-Ref Toxicant ~ ')End Date: 2/19/99 12:00 labid: CTI-Toxicology Laboratory S.imple Type: NACL-Sodium chloride Sample Date: 2/12/99 Protocol: EF-EPA /600/4-91/002 Test Species: PP Pimephales promela:.; Comments: Conc-mg/L 1 2 3 4 Control 0.5160 0.4450 0.4880 0.4720 1000 0.5490 0.5820 0.5780 0.5930 2000 0.4530 0.4270 0.4430 0.5480 4000 0.1070 0.1150 0.0610 0.1900 7000 0.0000 0.0000 0.0000 0.0000 10000 0.0000 0.0000 0.0000 0.0000 Transform: Untransformed isotonic ~'~ Cone-mg/L Mean N-Mean Mean Min Max CV% N Mean N-Mean Control 0.4803 1.0000 0.4803 0.4450 0.5160 6.187 4 0.5279 1.0000_ 1000 0.5755 1.1983 0.5755 0.5490 0.5930 3.262 4 0.5279 1.0000 2000 0.4678 0.9740 0.4678 0.4270 0.5480 11.665 4 0.4678 0.8861 4000 0.1183 0.2462 0.1183 0.0610 0.1900 45.180 4 0.1183 02240 7000 0.0000 0.0000 0.0000 0.0000 0.0000 0.000 4 0.0000 0.0000 10000 0.0000 0.0000 0.0000 0.0000 0.0000 0.000 4 0.0000 0.0000 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distnbution (p > 0.01) 0.93403 0.844 0.86025 0.56563 %artlett's Test Indicates soual variances (p = 0.33) 3.43556 11.3449 Linear interpolation (80 Rosamples) Point mg/L SD 95% ClJExp) Skew IC05 1438.98 249.773 1171.05 2457.86 1.1136 IC10 1877.96 211.78 1342.1 2391.59 0.0360 IC15 2109.05 149.633 1637.89 2473.8 -0.2281 1.0 ^ IC20 2260.09 118.831 1933.13 2604.03 0.1050 o,9 IC25 2411.12 106.544 2148.3 2734.27 0.1641 IC40 2864.23 82.6288 2663.04 3145.91 0.2916 0.8 f IC50 3166.31 83.4532 2969.13 3510.95 0.3208 0.7 ; O.6 - $ 5! E.04: l l0.3_ 0.2 ; y 0.1 0.0 < j 0.1 - '. / -0.2 ' 0 2000 4000 6000 8000 10000 Dose mg/L ~ P:ge 1 TexCale v5.0 Reviewed by
] COMMONWEALTH TECHNOLOGY, INC. Laboratory Division .3 .) CHRONIC REFERENCE TOXICANT TEST DATA Ceriodaphnia dubia 2000 - 1800 - +2 SD 1600 y /' > +1 SD U$ia; y 1200i y .i so 1000 2SD 800 s0 k khhh h h 'k as sssa sssa!ss i ---o o 6 o 5 o o o - - - o o o Test Date Dates Values Mean -1 SD -2 SD +1 SD +2 SD 08/07/97 1367.0000 0 0 09/97 125e.0000 1341.5000 2233.0111 1454.5223 4389.9889 1488.4777 10/17/97 1465.0000 1362.6667 1258.0993 1153.5319 1467.2340 1571.8014 11/03/97 1393.0000 1370.2500 1283.5345 1196.8189 1456.9655 1543.6811 12/02/97 1443.0000 1384.8000 1302.9575 1221.1149 1466.6425 1548.4851 01/27/98 1304.0000 1371.3333 1291.0422 1210.7511 1451.6245 1531.9156 02/18/98 1583.0000 1401.5714 1293.0697 1184.5680 1510.0731 1618.5748 03/09/98 1734.0000 1443.1250 1288.5145 1133.9040 1597.7355 1752.3460 04/17/98 1276.0000 1424.5556 1269.5724 1114.5892 1579.5387 1734.5219 05/1W98 1337.0000 1415.8000 1267.0804 1118.3609 1564.5196 1713 2391 06/1W98 1125.0000 1389.3636 12232 509 1057.1381 1555.4764 1721.5892 07/14/98 1102.0000 1365.4167 1186.6250 1007.8334 1544.2083 1722.9999 O & 22/98 1151.0000 1348.9231 1167.7075 986.4920 1530.1386 1711.3542 09/18/98 1331.0000 1347.6429 1173.4707 999.2985 1521.8150 1695.9872 10/01/98 1129.0000 1333.0667 1155.9902 978.9138 1510.1431 1687.2196 11/02/98 1295.0000 1330.6875 1159.3509 988.0143 1502.0241 1673.3607 12/02/98 1306.0000 13292353 1163.2313 997.2274 14952393 1661.2432 01/04/99 1356.0000 1330.7222 1169.5512 1008.3802 1491.8932 1653.0642 02/05/99 1713.0000 1350.8421 1171.3306 991.8192 1530.3536 1709.8650 02/12/d9 1533.0000 1359.9500 1180.5415 1001.1330 1539.3585 1718.7670 g 1.
Ceriodaphnia dubla S RVIVAL AND commDnwealth Technol0gy, InC. REPRODUCTION TEST DATA SHEET cex;ng$t n?g$nTe"$ a"oso"3 2g2, EPA 600/4-91/002, Method 1002.0 (606) 276-3506 Test Type:_ Reference Toxicant Datemme Test inniatod: ) -/2 M
2. % / Mn/-!
Toxicant:_ Sodium Chloride Datemme Test Terminated: 2 9 9 I 2- 0, ~. I Bio No.: 7 96/ Dilution Watw Used: 9.' / /fW Date/ Time i..ltfal Sartple Collected: 3-/2-99 .2 P/M Source Cuhure Date: 8/3d Time Renewed on Day: 1:_//'OOhA 2; I/ c04 3: 9 '.3 0 4 4: 9 ' 3DA%: 9:364: /3:COPA7; IDM2~. / j Number of Uve Young For Each Test Day (X denotes dead aih) Nacl Test Total No. Total No. l Conc. Container Sample Date or Dilution Water Batch Number og gy, og % ("**
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oao oao 02z cn-op- 022 on 1 2 3 4 5 6 7' 8' 1 0-O O 5 0 ll l6 52 [ 9 2 L( 2 0 o t) 4 ,o 3 0 o n ( O lo 12 0 o o 14 o 9 '12 6 b Corns to) 5 6 I O D // O P -F 14 hk@M#3N 6 0 0 0 5 O l() /J2. 49 g g e o o o 4 n 9 I.2 A.5 s 0 0 o 5 o /o /2 .24 I i 10 0 0 O S () 8 I( A4 0 No.of Uve Aduhs (O (O jg JO [g fa l /g l l ^ Number of Uve Young For Each Test Day (X denotes dead adult) Nacl Test Total No. Total No. Conc. Container We Date or Dilution Water Batch Number of gy, og uy, ("*4
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Young Pw Young w .p. 1 2 3 4 5 6 7 8 o o O r O 7 la 26 (s%#j NNLA 12 o O a 4 6 F (2 34 13 0 o o o 5 9 O 13 iiigi;:nM 1< 0 0 O O y 9 (lp 2% Q"L%%% sm 15 0 O O /-/ .O 9 14 Z4 8 *": 16 O O p% g 7 (( 22 jgg@ = 0'D n 4 <n 9 12 24 E* 18 0 0 0 5' C 4 I4 28 Pdh3@l$ 19 6 o D g/ O EP' /0 22 $nM$ 20 0 0 0 Al C) 9 (T 28 24l No.of M Wts 10 lo l l0 /O lO IO /A I N EERREEEE EE
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Ceriodaphnia dubla SURVIVAL AND CDmmDnwealth TechnDIDgy,Inc. REPRODUCTION TESTDATA SHEET 2520 Regency Road EPA 600/4-91/002, Method 1002.0 t,xington, gentueuy a050s.2g2, (606) 276-3506 TestType:_ Reference Toxicant Date/Tirre Test initiated: 2-!7 ' OM Toxicant:_Sodiurn Chloride Date/ Time Test Terrninated: Pt 4 '11 l 2 cm a ') Bio No. 760f Dilution Water Used:_91/ P-W ~ Date/Tirne inrtial Sanple Collected: k- (7 ';P=t Source Culture Date:_o/Jo Nurnber of Uve Young For Each Test Day (X denotes dead adutt) Nacl Test Total No. Total No. Conc. Container Sample Date or Dilution Water Batch Nurnber W Uve ofUve
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Y "",S Por Yo g Per 1 2 3 4 5 6 7 8 21 0 0 0 U O 9. Il 3 @Q 22 o o O Ll O l2 24 khygg $f[h{ 23 0 o o a 3 9 12 A.b 24 o O O 6/ O f is 2 85 jgg; m 2s a o o y h 9 l3 15 yyggff 16 0 0 0 0 3 8 15 ll
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27 Q Q 0 g g } (( 2l v 28 0 n O 3 g ll 2) 29 6 O O 3 b y 1; pl aw_ m D o O 4 0 9 /G 2R 25 5 No. of Uve Adults l [Q fg lp l lgllg l/0 i /O l 0 Nurnber of Uve Young For Each Test Day (X denotes dead adutt) Nacl. Test Conc. Container Sarnple Date or Dilution Water Batch Number Total No. Total No. og gy, ,, gy, M No. a/b ogg 2[f g/Iy 7 jlgg og2 You Per Young Per R p. 1 2 3 4 5 6 7 8 31 f) O o O 4 IB ?* [gf* 32 0 0 0 4 0 7 0 22 33 0 0 0 4 O q ig 1G
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34 /) O O O ff f IQ 20 ?. 35 b O O O 5 '7 ll lb J 3s o o o 4-O F /g zg gpMf 37 0 O O 4 0 ID .2 l ~,; 38 0 0 O L/ 9-O 13 23 f* 39 0 0 0 Lj 0 ] I4 26' 40 0 o O f a q q3 6) jyz No. of Uve Adults ll0 l [g /g l / g ( /() l[d l fg l gjQ$^$fg7]$l}$ Aa*52 P@ l M/ MI JJ MM l AB l Ihis/ l Kt/ Bh/ Corrrnents: pwpidataproc\\ bio \\formsk:chr-rt.sht ~
Ceriodaphnia dubla SURVIVAL AND CDnm onw c nD Dy, Inc. n no REPRODUCTION TEST DATA SHEET Lexington. Kentucky 40503-2921 EPA 600/4-91/002, Method 1002.0 (606)276'3506 Test Type:_ Reference Toxicant Date/Tirne Test inhiated:.2-/7-N 2 9 Toxicant:_ Sodium Chloride Datomrno Test Terminated: 2di~% $ IED I Bio No.: 780/ 1 Dilution Water Used: W/ 8 4) Datomrne initial Sarrple Collected;.2-/7"*f') Source Cu!!ure Date: 8/ ad Number of Uve Young For Each Test Day (X denotes dead aduh) Nacl Test Total No. Total No. Cone Container We Date w DHution Water Batch Ner og uge of Uve (mpy No. o/j) a//3 y((g pf >, gfI Wjjg g[f) Young Per Yo ne nc 1 2 3 4 5 6 7 8 41 o o o o 0 0 to 2. SiindEe
2 o
O o 3 0 5 0 8 $$bik 43 b () yo f(f~Q o ~ 0 o 4 0 7 il 22 W 44 6 1 45 0 0 Xn O T*
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O O O l+ b 2 IS 47 0 0 g } Q 5' q j'2 48 0 o o 4 o 6 (O jo 3 48 0 o o a O q q ,,t( w 50 0 O O 4 0 '7 10 af (L 9 No. of Uve Adults jl /6 l[() [h g g 8 p l Number of Ove Young For Each Test Day (X denotes dead acbit) Nacl, Test Total No. Total No. Conc. Container Sample Date w Dilution Water Batch Number of Uve of Uve ("*4
/s %
4/4 2/fg a/m t#2 3/,3 Young Per Young Per / 1 2 3 4 5 6 7 8 51 g 7 @lsb?@} Mt$ O 52 0 y O 53 0 Y O ? 7 54 0 Y O 2600 55 O y ,m, p i la ~ On t*L** 56 6 f) O g D Q 0 0 lEQ\\} 57 6 y O ' -Ik 58 )( 0 59 X o 'E a 60 0 O o No. of Uve Adults l } ll lJ l {., l / ly $;g$fM7%%@$$$ Myst M % l,Q l M l h M lMd dh j [)- N Approved By: Convnents: moera;..._ ;asy
1 COMMONWEALTH TECHNOLOGY, INC. Laboratory Division i Daphnid Survival and Reproduction Test-Reproduction Start Date: 2/12/99 14:00 Test ID: 7801 Sample ID: REF.Ref Toxicant End Date: 2/19/99 12:00 labid: CTI-Toxicology Laboratory Sample Type: NACL-Sodium chloride Sample Date: 2/12/99 Protocof: EF-EPA /600/4-91/002 Test Species: CD-Ceriodaphnia dubia Comments: Cone-mg/L 1 2 3 4 5 6 7 8 9 10 Control 32.000 21.000 27.000 25.000 19.000 27.000 23.000 25.000 27.000 24.000 600 26.000 24.000 13.000 27.000 27.000 22.000 24.000 28.000 22.000 28.000 900 24.000 24.000 23.000 25.000 25.000 21.000 21.000 22.000 21.000 29.000 1300 25.000 22.000 26.000 20.000 23.000 26.000 21.000 23.000 25.000 31.000 1800 2.000 8.000 0.000 22.000 0.000 18.000 17.000 20.000 21.000 21.000 2600 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 Transform: Untransformed isotonic Cone-mg/L Mean N-Mean Mean Min Max CV% N Mean N-Mean Control 25.000 1.0000 25.000 19.000 32.000 14.484 10 25.000 1.0000 600 24.100 0.9640 24.100 13.000 28.000 18.705 10 24.100 0.9640 900 23.500 0.9400 23.500 21.000 29.000 10.662 10 23.850 0.9540 1300 24200 0.9680 24200 20.000 31.000 13.038 10 23.850 0.9540 { 1800 12.900 0.5160 12.900 0.000 22.000 72.301 10 12.900 0.5160 2600 0.000 0.0000 0.000 0.000 0.000 0.000 10 0.000 0.0000 Muxiliary Tests Statistic Critical Skew Kurt Wapiro-Wilk's Test Indicates normal distnbutJon (p > 0.01) 0.93771 0.93 -0.699 0.92818 Bartlett's Test indicates uneaual variances (p = 4.39E-04) 202847 13 2767 Lineer interpolation (80 Rosamples) Point mg/L-SD 95% CL Skew ICOS 1304.57 395.336 268.178 1376.04 -0.1254 IC10 1361.64 241 254 536.355 1493.96 -2.1835 IC15 1418.72 66.6582 1329.04 1596.11 1.1892 1.0 ^ IC20 1475.8 832632 1377.89 1724.15 1.4328 g,,, IC25 1532.88 97.0628 1426.65 1823.92 12788 IC40 1704.11 118.539 1548.13 1979.14 0.3637 0.8 IC50, 1824.81 129.932 1633.9 2082.62 0.0043 0.7 $ 30.6 c g 0.5 h 0.4 0.3 - 02 01; 0.0 : C ' ~ 0 1000 2000 3000 Dose mg/L P29e 1 ToxCalc v5.0 Reviewed by: .-..,,.4.. ~
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SHORT-TERM CHRONIC TOXICITY TEST commonw::alth Tcchnology, Inc. TEMPERATURE RECORD 25 R*9*"'Y R ** Lexington, Kentucky 40503-2921 (606) 276-3506 Discharge: M h6 TX' A AF Test Initiation Date / Time:,2- /2-9 9 2,'oo j3, Location: Test Protocol Temperature: 2t-t 1*C Bio Log No.: /Sh r Test Organism: @ dubia?f. promelas (circle one) M. beryllina. M. bahia Other j$ampi@% ' w/*" ss $Temgieratsretc)1 - "O ^ Mfen. catjg $5 j DaNi /05/ 5 liDaN3j { Day 4l iDAyS3 3Dayiij (DaE7} (dhpl8j C w eoL M0 0 3. G Jqg a4 3 24.T .25.1 9ll.g '14,o le O O N'D 03.5 Wi. G .? 4. 6 .2d.$ 2.T O DQ (, AO 900 0[o l MS 24 G
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1[. ( 04.9 00 P) 's Analyst h /43 UN dC JJ Md /Hj k[ ummmmmmmmmmmmmu ammmmmmemummmmum mummmme mummmmmeammmmmmma-mammmmme ~ c~ ~: fnun(ny)= M9'(ny-w) k4 l0fr oS Ya,,yw<Aw<- Coitdevl o c c ~ *-k y y-c 3-dy l ],ec w .h /d Qf (, e vJJ& m i,, resp,,a % L ~ .t.J c.L.,,ie.2.rpe. j G' ~.'.
h S58990302001 From: - Moses. Jennifer sent Mondoy, March 01,1999 2:43 PM fo: Bodine. Debro J.: Strictdond. Lomer T. Jr.: Russell. Cindy: 'poul.potterson@ctienv.com' subject FW: Sequoyoh Nuclear Plant (SQN) Chronic Toxicity Tests -Reply For your information/ files.
d. R oses From:
Chris Moron (SMTP;cmoron@ moil.stote.tn.us) Sent: Monday. Moren 01.1999 2:36 PM fo: jmoses@tvo cov
Subject:
Secucyon Nuclect Plant (SQN) Chronic Toxtetty Tests Reply
Jennifer, Considering the circumstances, the Division will accept the results of the referenced test. Please feel free to e-mail or call me at (615) 532-0672 if you have any questions.
Chris Moran >>> " Moses, Jennifer" <jmoses@tva. gov > 03/01/9912:05pm >>> Commonwealth Technology, Inc. (CTI) TVA's contract laboratory for toxicity testing services, notified us that the samples to be used for test renewal on Day 4 (Saturday, February 27) were not delivered by Fed Ex. Samples used for test renewal the previous day were used a second time on Saturday. All holding times and temperatures met EPA requirements. Samples for use on Sunday and Monday were received as scheduled. Because the SQN permit calls for daily grab samples, we need your approval to accept the test as valid. Please respond via email or call me at 256-386-2518 If you need additionalinformation. Jennifer Moses Tennessee Valley Authority Environmental Research and Services CTR 2P-M Phone: (256) 386-2518 Fax: (256) 386-2031 cc: EDMS, WTC A-K T'*WW 14 3f' *9 ", p-M9: 't's -se iges r w e -l-m-em 9een e m g.gqqe,v m 3 ,g
T'ennessee Valley A~th?rity CTR 2P. Muscle Shoals, AL 35662-1010 FAX ss-, Number of pages including cover sheet: 11 l To: From: Chris Moran Jennifer Moses l State of Tennessee Senior Toxicolonist Tennessee Department of Environment and Tennessee Valley Authority i Environmental Research and Services l j Phone: (256) 386-2518 Phone: (615) 532-0672 i Fax phone: (256) 386-2518 l Fax phone: (6L5T 5 30 -- ond A g l REMARKS: E Urgent @ Foryourreview @ Reply ASAP @ Please cont.1cet l The following pages are benchsheets and data analyses from a Sequoyah Nuclear Plant (SQN) fathead minnow chronic toxicity test of Outfall 101, conducted by Commonwealth Technology, Inc. from 2-23-99 to 3-2-99. i Sporadic mortality was exhibited throughout the test, resulting in a dose response which did not meet the requirements for use of point estimation to determine an 1C3 based NOEC (i.e. "the response was NOT monotonically non-increasing, where the mean response for each higher concentration was less than or equal to the mean response for the previous concentration"). Hypothesis based analyses were, therefore, selected to analyze the results of this test (EPA -600-4-91-002). The compliance endpoint of 48.98% (49%) showed no statistical difference from controls in survival or growth. Survival in the 25% concentration was, however, found to be statistically l less than controls. The 80% treatment was also significantly less than controls for survival and l l l
COMMONWEALTH TECHNOLOGY,INC. La boratory Divisio n Latvet Fish Survival and Growth Tess Start Growth __ h Daw: 2/23/99 11:00 Test ID: 7829 Sempe 10: TV TVA End Date: 3/2/99 11:00 LapID: CTITomoolo0y Laboratory Sample Type: EFF3 Power Plant Sample Date: 2/22/99 09,48 Protocol: EF.EPANiOQ/4-91/002 Test Species PP-Pirnephales promelas Comments
MWah Nuclear Plert Cone %
Y 2 3 4 Control 0.5820 0.5760 0.6040 0.5410 25 0.3480 0.3810 0.2800 0.0720 39 2 0.3800 0.5550 0.5410 02520 49 0.4440 0.5070 05540 02910 80 0.1950 0.4310 0.4510 0.3330 100 0.3200 0.3840 0.4240 0.5020 ~Translerm: L;. _ '_- :: 1eolorac Cone % Mean fH4een Itsen Man Max CV% N Mean N Mean Contrat 0.5708 1.0000 0.5708 0.5410 0.8040 4 830 4 0.57D8 1.0000 25 0.2670 0.4678 02670 0.0720 0.3610 50.056 4 0.3810 0.6675 30 2 0.4270 0.7441 0.4270 02520 0.5650 34.338 4 0.3810 0.6675 49 0.4400 0.7867 0.4480 02910 0.5640 25.516 4 0.3810 0.5675 80 0.3525 0.6176 0.3525 0.1950 0.4510 33.185 4 0.3800 0.6658 100 0.4075 0.7140 0.4075 0.3200 0.5020 18.695 4 0.3800 0.6654 ^ di==y Tests Statisme Critices shour Kurt Shapiro-Wilk's Test indicases rennst thstntulaen (p = 0.01) 0.91588 0.884 0.6709 0.5424 Bartlett's Test inchcotes W venances (p = 0.27) 6.43867 15.0863 IJneerInterpotahon(80 Resemples) Point so se% CL(to) amour _ _ _ eCOS* 3 700 0.573 2.728 5.817 0.6872 1C10* 7.520 1.147 5.457 11.634 0.6872 IC15' 11.280 1.720 8.185 17.451 0.6872 1.0 IC20* 15.040 2.294 10.913 23.266 0.6872 1C25' 18.799 8.120 13.842 99.546 3.8782 1C40 >100 0A-IC50 >100 g7;
snsheeses 1C esamete less than the lowest concentrabon 02-0.5
,I*,
0. :
I E l. .i. ~ 0.5 - 02 0.1 { / 0.0 0 20 40 00 80 100 Dose % e. .. _.W , _ ~... TOTAL P.11
COMMONWEALTH TECHNOLOGY,INC. Laboratory Division I 1 1.arvel Faen survival and Growth _ Test Stort Growth -Start Dete; 2/59911:00 Test ID: 7830-1 SamgHe ID: - TV TVA End Date: S/2/99 11:00 Lab 10: CTI TQiLaboratory Sample Type: EFF3-Power Plant Sample Delp: 2/22/99 Protocci-EPA /600f4-91/002 Test Species PP-Pimephales promelas -* Commoner Sequaveh Nuclear Plant take Cone % 1 2 3v4 I Control 0.5630 0.6760 0.6040 0.5410 f 100 0.5170 0.4870 0.4430 0.1910 Transform: Usereneformeel 1-Tesed Cone-%. RAsen N tIsen Resen sein IAnz CY% N t-Stet critical MSO ) Contret 0.5706 1.0000 0.5708 0.5410 0.6040 4.630 4 -
100 0.4095 0.7175 0.4095 0.1910 0.5170 38.338 4
2.134 1.943 0.0111 l 1 AsstilsoryTests _ Statione Critleet Skew Kaart ~She0wo Wurs Test inchcotes normal estribudon (p > 0.D1) 0.84176 0.740 1.7064 3.87427 i F-Tomir:Matos sensel venances (p = 0.02) 31.7112 47.4683 weie Test (in 0.08) --+
t Test indseles sign # cant chfferences
~ 0 9 e e b e. 8
COMMONWEALTH TECHNOLOGY,INC. Laboratory Division i l I LarwelTsah Survival anal Growth Tes>4ervtwel Steet Date: 2/23/99 11:00 Test 1D: NI Semp6e ID: TV TVA-End Date: 3/2/99 11:00 LasID: CT)-Tossoology L.t e.d.y SampleType: EFFS. Power Plant PP-P' neohaies Oromelas Semple Date: 2/22/98 Protocol EPAlt00/4-01/002 Test W: n -+ Comments-
r_^32, Nucieer Plant /
Cone-% 1 2 3 7 C5nnot 1.0000 1.0000 1.0000 T.0000 100 1.0000 1.0000 1.0000 0.3000 Transform: Anisen Square float Rank 1-Taliess Com % Mean 44esen Mean Mia Max CY% M Sum Critical Control 1.0000 1.0000 1A120 1.4120 1.4120 0.000 4 100 0.8250 0.8250 1.2039 0.5796 1.4120 34.689 4 16.00 11.00 l Ateilnery Tests statsobe critical Skew Kurt Shepro Wdits Test andcasse non-normeldistribuson (p a 0.01) 0.7064 0.749 2.0367 4.9 Equale of venance cannot be confirmed
.,_ _ _^ =^: Test (14 ell, ILOS) 4 Wiloonon Two Sample Testineontes no monfmant allferences O
.> ene kcue by:I Pass i TascMe vs.0 .p ImAgase .me,.m.e
COMMONWEALTH TECHNOLOGY INC. Laboratory Division Larval Fishhival and Growth Test _-_ tort Growth S Stort Date: 2/23/90 11:00 Test 10: 7830-U Sample ID. TV TVA End Date: 3/2/90 ItM 1.ab 10: CTI Toncology hrwi Sample Type: EFF3 Power Plant Semple Dels: 2f22/99 ProtoceAt-A/600/4 91/002 Test Spec #es* PP-Punephates promelas + Comments: Mmah Nuclear Plant /Opstroom'l ~ Cono-% - i 2 3 N, Control 0.6620 0.5700 0.6040 0.5410 100 02090 0.3820 0.3850 0.4070 l _ Transform: Untransformed 1-Tenee Cone % ___Mean N-Mean mean Mht Max CV% N 1-Stot Criticel _ MSD Control 0.5706 1.0000 0.5706 0.5410 0.6040 4.630 4
100 0.3408 0.5970 0.3408 0.2090 0.4070 26.334 4
4.917 1.943 0.0043 Aumahery_T eae Stettstic Critical __ skew Kurt ShapuD-Willt's Test indestos normel distributson (p > 0.01) 0 37388 0.749 1.5675 3.06231 F Test Indicates & &ces (p = 0.0D 11.S_32 47.4683 l Hypothasis Test (1-tall. 0.05)__ y We 1 Test indicates eigrufscant allierences Q) PasM 1 TonCalcv50 Rev~ewed by: Yi
COMMONWEALTH TECHNOLOGY,INC. Laboratory Division t.anet Feeh Survivel and Growth Test-surv6 vel 9 tart Dele: 2/23/99 11:00 TestID: 7830.U Sample ID: TV TVA 64 Date: 3/2,9911:00 labid: CTI Tarcology Laborosory Sartple Type: EFF3-Power Plant Sample Dees: 2/22/99 Protocol N60W4 91/002 Test Species: PP.Pimephales prometas % Comments- %woh Nuclear Plant / Cono % i 2 3 g ~ Cormon 1.0000 1.0000 1.0000 1.0000 100 0.4000 0.7000 0.7000 0.0000 7tansforne AsesinSquare Aoor 1-Telled Cono % RAeon N.40sen Resen niin lles CV% _N t-S1st Critical MSO Corsol 1.0000 1.0000 1.4120 1.4T20 1.4120 0.000 4 '100 0.6600 0.6600 0.9435 0.6647 1.1071 19.164 4 5.176 2.353 0.0193 l i Aieuliery Tests Stabetse Criheel. Skeur Epst WW81E's Test indiceses normel estrbuson (p > 0.01) 0.79204 0.7W9 1,4589 4.16758 Eausier of variance connot ce cenarmed L ff ^ Test (1 esit,0.05)
P W t Test mdicates signecant efferences Beviewed byk Po9 1 TonCale vs.0
%=
l Analysis of 7-day. Larval Growth Test SON 6, 6 Starting Date: 02/23/99 v.0 9 Analysis of Dry Weight ( ms) Data Dunnett 's Test No transformation applied before data analysis, i For this set of data, the minimum significant difference is 0.09 This represents. a 16.40 % reduction in Dry Weight (mg). T= 2.29 ALPHA = 0.05 Dry Weight (mg) Data Replicate TREATMENT 1 2 3 4 5 6 7 8 9 10 MEAN S 1 CONTROL 0.56 0.58 0.60 0.54 0.57 2 39.2 0.40 0.56 0.54 0.42 0.40 3 49 0.44 0.56 0.55 0.49 0.51 4 100 0.53 0.43 0.42 0.50 O.47 istorisk ( * ) indicates values significantly less than control ( CONTROL ). Analysis of Variance Source DF Sum OF Sq. Mean Sq. Cale F F( 0.05 ) Among .3 0.024 0.008 2.430 0.1158 Within 12 0.040 0.003 Total 15 0.000

~~------

>+ pp ,m.. y ~ -6
Analysis of Survival SON 3, oW Starting Date: 02/23/99 Survival (%) Data

~~--------------------------------------------------------------------

Replicate Trootment 1 2 3 4 5 7 8 9 10 Mean

6----------~~-----------------

DNTROL 1.00 1.00 1.00 1.00 1.00 5 0.90 0.80 0.90 0.40 0.75 9.2 0.90 1.00 1.00 0.60 0.88 ) 1.00 0.90 1.00 1.00 0.98 3 0.40 0.80 0.80 0.70 0.68 )O O.60 0.90 1.00 1.00 0.88 Steel 's Many-One Rank Test No. of Critical Treatment Replicates Rank Sum

Rank Sum

25 4
10 10 39.2 4 10 14 49 4 10 14 80 4 10 10 100 4 10

14

Values less than Critical Rank Sum significantly less than the control (CONTROL
). Jew *
FATHEAD n0NNOW uzo g LARVAL SURVIVAL AND GROWTH TEST t.ew Kvausy *0s03-2921 EPA 800447A302, AGE 7 HOD 1000,0 (606) 27N Edll, /Ml,e.h Desarfene Test Termmated 6-2 M ll:so Dischargw/Locaten: I#nferl/af
t Qo%d_ Ar.ds,.
PIM 64il lel' organ sm enten mese: Od2 Bio. L.og No: ~+63o intel Orgarusm A08: I29 h DateTime Test initored .1.7.3 -9 T 10... A ouugon Waser Used. U l S Iv. Diunon Waeor Baton Amber on Day: 1:____od 2: 021 3:ddO. 4: 03i 5:0.3I 6:M 7: OM Time of Day Renewed on Day: 1: //:ceA f 2: II.'00Arri 3: tr'#_84e 4: 3"d w: tO' og:-4fetPN}) d o , Sample ID Test ~ No. of Uve IJrvas (Doys) Tin WL of Wt of Total Average Aver *9e Cent sample Deens No. Tin & Tm WL of wt. of Wt. for " * ' % %W W% %b Q N N 'T (**) 1 2 3 4 Y 6 7 oi lo 10 10 D IO % 6 at len.9oJ loao.w 3.3 site..Too " l-02 to la_ to D 10 to 9 as 999.39 999.95 3. gqfs.2ey Y_ 1 I60*4 03 ID lo to to 10 IO LO. 03 996.04 9%o0 4.04. o 424 c=__=4 lb (0 10. p lO (O Q 3't 993.90 49,2.2G 5.00 / _e.goa
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.7 Of to ID_ /0 10 10 IO LO 96 rs't. 47 IM3 80 4. t i o.9e 7 i =E M' 88*/*
12t Ag7 4.93 o.yyy [ BEl@j 07 Io 10 to /o l0 /0 10 27 low.3o t W I0 10 la' IO Q 4 3 w 09s. co IM4.og I.91 0.I91 0." l0 29 10 10 10 lb (o f 4 34 10 % l8 9 IM.49 2.09 6.309 30 (0 10 /0 (f 9 8 3-30 9F6.63 9 23.01 3.63 0.340 " %% '"'/- 3r to I0 to 9 9 7 5 21 tott.or l245D 3.ss 0 3sr i as lo 10 le to lo p g 2a get.es 9ts.4 4.o 2 o.vo ; o.39; 851E1R M l
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LARVAL SURWVAL AND GROWTH TEST tuingen.Kenney sosasts21 EPA 600V4 9fADG2, AGETHOD 1000.0 (606) 276 4506 Dinhwo=>tocanon:hwJsa.14/I,, &ff.v% Davnm. Tot T.,minai.o: '2-2 9 9 il:00 > Rtw,o hr4 kulser Ph.f AdQIl le r ' _ organi.m satch Number-02.L1 Bio. tog No: WO inimalOrganism Age: U4b 0 (p0 AA h Water Used: 9! /[I'. Date/Twne Test Inteleted:_ _ _ _ 9 -J.H 8l Dducon Water Batch Wmtier on Day. 1:_c19 2: 029 3: 030 4: car s:g s: 032 7: o 32 Time of Day Renewed on Dey: 1: //:#ee. 2: //.'co_ Ave 3: ll'oegn. 4: 3 '. lb,.5:__9
6: [O'ocle,,,,7:P_h Sample ID Test No. of Uvo Larvae (Days)
Tin WL of Wt.of Total Average Average Cont. _ Sample Dates No. Tin & Tin Wt. of Wt. of WL for __%W %/ % % ag 4, py yy p,,y (ms) T'aia= 1 2 3 4 5 6 7 f to to to to l0 80 lb l 990.3 3 9 84.% 5.so, o.ss 9 5-j Q. to to to 10 IO 10 10 3 Istu,r i518.92 s., c,. e. s,s EL 2 (66*l 3 to 10 to 10 10_ (O \\0 3 %It_o 984.%
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I4 16 ID to lo l0 80 9 l't 999.sr 424.s1 s.one5 s so y gn 5 ~ 15 ta 10 IO 50__ IO iO 10 ft 996.or 990.5% 5.5 4 N' b.ss y ?% 16 10 10 10 ID _Lo 6 h Il 993.36 890 M 3 9 fo? o 99I 0 449 LLl0 l0.j0__1 k 4.,D_, 99G og. 994,0t I.95a? o.19s 1 if to /D 10 Am1Tm ger.r. 991.2i w o.4s t to 10 10 10 10 nf 8 11 990.71 5 4.51 Y 0 4t l --= l4 0 to 10 io a r > +
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le ma /w Notes: l Approved By T/ =pegege.ap ee =
s the 100% concIntration wcs significant for growth. Upstream and raw was iitake samples showed similar pattems of mortality and also showed statistical differences from test contre'.s when analyzed using the Homoscedastic t-Test, thereby demonstrating amb;snt wate, toxicity of some sort. Survival and growth were reduced significantly in the Upstream soplo end growth was significant in the intake samples. We believe the randem occurrence of mortality throughout the test and the resulting statistically significant reductions from controls were related to ambient receiving stream water used for cooling, NOT toxicity present in SQN Outfall 101. Microbial pathogens present in the river are a likely cause of this type of highly variable survival response. Following your review of the data, we request you consider two options:
1) Rule the test was a pass since the compliance concentration did not exhibit significant reductions in either survival or growth, or

2) Rule the test invalid and do NOT count as a violation of the SON permit, with a repeat test to be conducted as soon as one can be scheduled with the contract laboratory (initiated within the next two weeks).
Please call me if you need to discuss the data further. If you determine the test will be a permit violat'on you will also need to contact Debby Bodine at Sequoyah ASAP so she can handle the required notifications within 4-hours of learning of a non-compliance. Debby's phone number is (423) 843-6700 and her p.sger is 1-800-323-4853, Pager # 70343. Jennifer Moses
F Bodine, Debra J. From: Moses, Jennifer Sent: Tuesday, March 09,19991:44 PM To: 'Chris Moran' Cc: Bodine, Debra J.
Subject:
RE: Sequoyah Fathead Minnow Chronic Data Review Reply We had two tests under discussion Friday and I guess we got them mixed up. The test we were trying to repeat before the plant outage, but later determined was not a "significant" test failure, was the Bull Run Fossil Plant daphnid chronic test. The Sequoyah fathead minnow data was what i faxed and what we determined was not statistically different at the compliance concentration (48.98%), and therefore not a failure based on your email below. Thank you for your help with these issues. dTennifer Ot}oses Tennessee Valley Authority Environmental Research and Services CTR 2P-M i Phone: (256) 386-2518 j Fax: (256) 386-2031 1 i From: chris Moran[sMTP:cmoran@ mail. state.tn.us) Sent: Tuesday, March 09,199912:59 PM l To: jmoses@tva gov
Subject:
sequoyah Fathead Minnow Chronic Data Review -Reply
Jennifer, I'm afraid I misunderstood your previous phone message I which you said the failure was not significant, and assumed that I did not need to review the data from the chronic fathead test. I apologize.
i After looking at the data, I believe the Division can be comfortable in " sticking to the letter" of the permit which requires compliance at 48.98% effluent. Since there was no statistical difference from controls at this concentration, we will consider the test a pass. If you have further questions, please let me know. Chris Moran Page1 -..-m... g
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Sequoyah Nuclear Plant (SON)- Notification of Noncompliance affecting Effluent Limitations - NPDES Permit No. TN0026450. Description of Noncompliance: The Lined Metal Pond, Outfall 107, " Metal Cleaning Wastewater and Storm Water Runoff" was discharged on 3/18/99. SQN NPDES Permit No. TN0026450 stipulates that Total Suspended Solids (TSS), iron (Fe), and copper (Cu) analyses are to be collected at the beginning and end of releases and that the resultant data be reported as a composite. End of discharge samples for TSS, Fe, and Cu were collected on 3/18/99 upon discharge termination as specified in the Permit. However, these samples were not transmitted to the Environmental Chemistry Lab soon enough to be analyzed within the 7-day maximum holding period for TSS as specified in 40 CFR Ch.1,136.3, Table ll (Required Container, Preservation Techniques, and Holding Times). The beginning of discharge TSS was sampled on 3/18/99 and analyzed within 7-days on 3/24/99 (6-days). The end of discharge TSS sample was collected on 3/18/99 and analyzed on 4/8/99 which exceeded the maximum holding time by 13 days. Both beginning and end of discharge metals analyses were performed within the 28-days specified in Table ll. Cause and Period of the Noncomoliance: Total time of discharge was 8 hours and 5 minutes which represents the period of noncompliance. End of discharge samples were collected at j 22:50 on 3/18/99. The Environmental Chemistry Laboratory was closed at that time which necessitated storing the samples in a refrigerator until they could be delivered. The samples were overlooked in the refrigerator until their discovery on 4/8/99 when they were promptly delivered for analyses. TSS analyses results are as follows. Total Suspended Solids Analysis ID Sample Date/ Time Analysis date/ Time Results (Permit Limit) Discharge Beginning 3/18/99 @ 14:55 3/24/99 @ 14:00 2 mg/L (30 mg/L) End of Discharge 3/18/99 @ 22:50 4/8/99 @ 11:03 2 mg/L (30 mg/L) l We feel that the results of the delinquent analysis is representative of the TSS inventory within the Lined Metal Pond even though it was not analyzed within the holding period. The Lined Metal Pond was discharged 4 additional times during the month of March. Those composited TSS i results are as follows. j Release Dates 3/1/99 3/5/99 3/13/99 3/16/99 TSS 2.5 mg/L < 1mg/L < 2 mg/L < 1 mg/L Further, the matrix of the Metal Cleaning Waste Ponds is such that sample stability is possible for samples refrigerated longer than the allowed 7 days. This is demonstrated by samples of the End of Discharge of the Unlined Metal Cleaning Waste Pond also collected on 3/18/99. Analysis ID Sample Date/ Time Analysis Date/ Time Results (Permit Limit) End of Discharge 3/18/99 3/22/99 @ 1100 8 mg/L End of Discharge 3/18/99 (backep) 4/08/99 @ 1325 9 mg/L Steos Taken to Reduce. Eliminate and Prevent Recurrence of the Noncompliance: Personnel accountability for the sample custody through analysis process is being emphasized. Stringent oversight of the process is being conducted as an interim measure until a new tracking system for the process can be completely implemented. m._. r.-
Our Environmental Technician, Ron Lowery, had a stroke in December and is still confined to desk work. From Thursday, February 25 until Tuesday, March 2, our other Environmental Technician, Wanda Allen, had the flu and was at home sick. On Sunday, February 28*, I and a person training on technician duties pulled a set of pre-release samples on the Linert Metal Cleaning Waste Pond. This set included pH, O&G, TSS, Fe and Cu which were all within permit limits. They were taken in exactly the same manner as discharge sample are taken but, instead of being discharged, the discharge was routed back to the Lined Metal Cleaning Waste Pond. On Monday, March 1, the person training on technician duties re-checked the pH and started the discharge. He thought the Sunday samples were in fact the start of discharge samples and did not pull an additional set. There was no rainfall or other influent to the Lined Metal Cleaning Waste Pond during the period between pre-release sampling and the beginning of discharge. End of release samples were taken as required. The sample results are comparable for pre-release versus end of release Analysis Pre-release Beginning of Release End-of-Release pH 7.99 7.78 NA O&G <5 mg/L NA TSS 3 mg/L 2 mg/L Fe 0.480 mg/L 0.560 mg/L Cu <0.01 mg/L <0.01 mg/L Under these circumstances, can we substitute the pre-release sample results for O&G, TSS, Fe and Cu for the beginning of discharge samples? 1
Nida, Di::dra B. From: - Bodine, Debra J. Sent: Friday, March 05,1999 3:20 PM To: Strickland, Lamar T. Jr. Cc: Nida Diedre B.; Osbome, Jerry D.
~~Subject:~~
Lined Metal Cleaning Waste Pond Discharge Explanation for the DMR I spoke with Terry Whalen today. He said that under the circumstances, the Chattanooga Environmental Assistance Center will allow us to use the pre-release samples from 3/1/99 for the beginning of discharge samples on 3/2/99 He asked that we include the etisched explanation with the DMR page for 107 and put: " Chattanooga Environmental Assistance Center Personnel concurred with the sampling of the 3/2/99 release as described in the attached page," LMCWPtoTDEC. doc i Page 1 i I -___~y .7-.,- y----. y7,3 - -
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Dear Ms. Haskin:

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