Text

NPDES Discharge Monitoring Rept for Period 980701-0930
	ML20154P551
Person / Time
Site:	Sequoyah
Issue date:	09/30/1998
From:	Bodine D; TENNESSEE VALLEY AUTHORITY
To:	;
Shared Package
ML20154P527	List: ML20154P524; ML20154P551;
References
	NUDOCS 9810230010
	Download: ML20154P551 (79)
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Channel Discharge Date: Diesel Range Organics Total Water Comments (mg/LY Hydrocarbons (me/L) 7/1/98 < 0.1 < 1.0 7/8/98 < 0.1 < 1.0 7/15/98 < 0.1 < 1.0 7/22/98 < 0.1 < 1.0 7/29/98 < 0.1 < 1.0 8/5/98 < 0.1 < 1.0 8/12/98 < 0.1 < 1.0 8/19/98 < 0.1 < 1.0 8/26/98 < 0.1 < 1.0 9/2/98 < 0.1 < 1.0 9/9/98 < 0.1 < 1.0 9/16/98 < 0.1 < 1.0 9/23/98 < 0.1 < 1.0 9/30/98 < 0.1 < 1.0 Trench Discharge Data Date: Diesel Range Organics Total Water Comments: (me/L) Hydrocarbons (me/L) 7/1/98 3.6 30 7/1/98 2.6 7 Back-up sample collected on 7/l 7/8/98 1.3 2 7/15/98 0.4 < 1.0 7/22/98 0.8 < 1.0 7/29/98 0.8 < 1.0 8/5/98 1.3 < 1.0 8/12/98 1.0 < 1.0 8/19/98 1.0 < 1.0 8/26/98 < 0.1 < 1.0 9/2/98 0.9 < 1.0 9/9/98 2.9 < 1.0 9/16/98 1.7 < 1.0 9/23/98 0.7 < 1.0 9/30/98 0.9 < 1.0

t 1. I October 1,1998 1 Debra J. Bodine, OPS 2B-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, SEPTEMBER,1998 REPORT Attached are two copies of the subject report for submission to the state of Tennessee with the DMR package you submit for SQN. The report provides results of compliance tests required under the SQN NPDES permit. SQN Outfall 101 samples collected September 8-14,1998, were not toxic to fathead minnows or daphnids (96-h LC50's > 100 percent and 7-day NOEC's, measured as IC23's, >100 percent). Exposure of test organisms to intake and upstream samples resulted in no toxicity to either species. Call me at (256) 729-2779 if you have any questions or comments following your review of the report. r 1 (pM Cynthia L. Russell Biologist Toxicity Testing Laboraton/ l TTL 1 A-BFN CLR Attachment L cc (Attachment): D. C. Wade, CTR 2P-M - R. D. Davis, WT 10D-K Files, WM, CST 17B-C sqn-0998m. doc l-I

e TENNESSEE VALLEY AUTHORITY TOXICITY TESTING LABORATORY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

1) Facility / Discharger: Seouovah Nuclear Plant /TVA Report Date: October 1.1998

2) County / State: Hamilton / Tennessee

3) NPDES Permit #: TN0026450

4) Type ofFacility: Nuclear-fueled electric ceneratine olant 1

5) Design Flow (MGD): 3266 0 Receiving Stream: Tennessee River (TRM 483.6)

7) IQ20 ( 4GD): 2992.4 Outfall Tested: 101

9) Dates Sampled- 09/08-14/98 y

10) Flow on day (s) sampled (MGD): 1622 1413 833 866 862 869 993

11) Site conditions: No unusual conditions recorted.*

12) Test Dates: 09/09-16/98

13) Test Type: Short-term Chronic-Definitive

14) Test Species:

Fathead Minnows (Pimenhalespromelas) Daohnids (Ceriodachnia dubia)

15) Concentrations Tested (%): Outfall 101 25 39.2 49 80 100 Intake 100 Upstream (TRM 486.4) 100

16) Permit Limit Endpoint (%): Outfall 101 96 hrs LC = 100 NOEC = 49 3

17) Test Results (%):

Outfall 101 Pimephalespromelas: 96 hrs LC3 > 100 NOEC (ICn) > 100 Ceriodaphnia dubia: 96 hrs LCa > 100 NOEC (ICn) > 100

18) Facility

Contact:

Debra J. Bodine_

19) Phone #: (423) 843-6700

20) Consultant / Testing Lab Name TVA Toxicity Testine Laboratory (TTL)

21) Lab

Contact:

Cynthia L. Russell Phone #: (256) 729-2779

23) Notes: Samples from Outfall 101 exhibited no toxicity to either species tested based on the applicable permit limits. Pertinent chemical data and summary of application is provided in Appendix B.-

Production / operation data will be provided in the event toxicity exceeds permit limits.

2 METHODS

SUMMARY

+ Samoles-

1) Sampling Point:

Outfall 101

2) SampleType: Grab

3) ' Sample Information:

Date/ Time Date/ Time Arrival Initial Date/ Time ID Collected Received Temp. TRC*' Used By (MM-DD/ Time) (MM-DDffime) (*C) (mg/L) (MM-DD/ Time) 101 09-08/0937 EDT 09-09/1135 CDT 1.1 <0.1 09-09/1330 CDT Intake 09-08/1055 EDT 09-09/1135 CDT 0.7 <0.1 09-09/1330 CDT Upstream 09-08/0957 EDT 09-09/1135 CDT 0.7 <0.1 09-09/1330 CDT 101 09-09/0851 EDT 09-10/1020 CDT 1.5 <0.1 0910/1340 CDT Intake 09-09/0747 EDT 09-10/1020 CDT 0.8 <0.1 09-10/1340 CDT Upstream 09-09/0910 EDT 09-10/1020 CDT 1.0 <0.1 09-10/1340 CDT 101 09-10/0855 EDT 09-11/0945 CDT 1.1 <0.1 09-11/1337 CDT 1.3t 09-12/1335 CDT Intake 09-10/0811 EDT 09-11/0945 CDT 0.7 <0.1 09-11/1337 CDT 0.6t 09-12/1335 CDT Upstream 09-10/0916 EDT 09-11/0945 CDT 0.9 <0.1 09-11/1337 CDT 1.Si 09-12/1335 CDT -101 09-11/0830 EDT 09-14s0945 CDT N/A N/A Discarded

Intake 09-11/0749 EDT 09-14/0945 CDT N/A N/A Discarded
t Upstream 09-11/0851 EDT 09-14/0945 CDT N/A N/A Discarded 101 09-12/0841 EDT 09-12/1515 CDT 1.1

<0.1 09-13/1325 CDT Intake 09-12/0749 EDT 09-12/1515 CDT 0.5 <0.1 09-13/1325 CDT Upstream 09-12/0943 EDT 09-12/1515 CDT 0.4 <0.1 09-13/1325 CDT 101 09-13/0838 EDT 09-13/1445 CDT 1.5 <0.1 09-14/1330 CDT Intake 09-13/0747 EDT 09-13/1445 CDT 0.6 <0.1 09-14/1330 CDT Upstream 09-13/0928 EDT 09-13/1445 CDT 0.6 <0.1 09-14/1330 CDT -101 09-14/0846 EDT 0915/1000 CDT 1.1 <0.1 09-15/1330 CDT Intr.ke 09-14/0739 EDT 09-15/1000 CDT 0.8 <0.1 09-15/1330 CDT Upstream 09-14/0829 EDT 09-15/1000 CDT 0.7 <0.1 09-15/1330 CDT

Total residual chlorine.

fSample storage temperature.

Samples were not received until 09/14/98. Samples collected 09/10/98 were used for renewal.

4) Sample manipulation: Samples were poured through a 64 pm screen into a beaker before warming to 25 1 C. Aeration was not necessary to bring sample DO levels down to saturation.

2 i

Test Organisms Pimephalespromelas Ceriodaphnia dubia

1) Source:

In-house Culture In-house Culture

2) Age:

0.5-22.5 hours 7.5-15.5 hours Test Method Summarv (See Appendix A for additionalinformation) Pimephalespromelas Ceriodaphnia dus.a

1) Test Conditions Static. renewal Static. renewal

2) Test Duration 7 days 6 davs (3 brood)

3) Dilution / Control Moderatelv Hard Modified Moderatelv Hard Water Synthetic Water Synthetic Water

4) Number Replicates 4

10

5) Animals per Replicate 10 1

6) Test Initiation 09-09-98/1330 CDT 09-09-98/1320-1335 CDT (Date/ Time)

7) Test Termination 09-16-98/1330 CDT 09-15-98/1320-1410 CDT (Date/ Time)

8) Test Temperature Mean = 25.0 C Mean = 24.8 C (24.7 C-25.4 C)

(24.1 C-25.5 C)

9) Physical /ChemicalMeasurements: Hardness, alkalinity, and conductivity were measured at the laboratory in each 100 percent sample. Temperature was measured in each test concentration daily. Pre-and post-exposure (initial and final, respectively) test solutions were analyzed daily for pH and dissolved oxygen in both tests. Post exposure test solutions were analyzed daily for conductivity in the fathead minnow test.

10) Statistics: ICu-EPA Bootstrap Program 3

TQXICITY TEST RESULTS (See Appendix C for Bench Sheets)

1) Results of a Pimepha/es prordelas_

Chronic 7-d Toxicity Test (Genus) (Species) (Type / Duration) Conducted 09/09/98 - 09/16/98 Using Effluent From Outfall 101 (mm/dd/yy) (mm/dd/yy) (number) Percent Surviving l Test Solutions (time intervals used - days) 1 2 .1 1* 1 6 2-Control 100 100 100 98 95 g g 25% Emuent 100 98 98 98 98 g g 39,2% Emuent 100 97 97 97 92 92 92 49% Emuent 100 100 100 100 1qq 100 100 80% Emuent 100 H 98 98 98 98 100% Emuent 100 100 100 100 97 97 97 100 100 98 98 98 M Intake 100 Upstream 100 100 100 100 100 100 100

96-Hour LC5o Value > 100%

Calculated TU Estimates < 1.0 TUa. 95% Confidence Limits Permit Limit 1.0 TUa Upper LimitliA Lower Limit NA ICu Mean Dry Weight (mg) Test Solutions (Replication) 1 2 3 4 Mean Control 0.496 0.571 0.703 0.605 0.594 25% Emuent 0699 0.575 0.594 0.668 0.634 39.2% Emuent 0.701 0.737 0.440 0.640 0.630 49% Emuent 0.693 0.719 0.655 0.698 0.691 80% Emuent 0.639 0 674 0.754 0.723 0.698 100% Emuent 0 633 0.680 0.645 0.529 0.622 Intake 0.849 0.679 0.555 0.600 0.671 Upstream _ 0.568 0 671 0 665 0.693 0.649 ICu Value > 100% Calculated TU Estimates i <l.0 TUc 95% Confidence Limits Permit Limit 1.0 TUc ~ Upper Limit NA Lower Limit NA ' NOTE: TUa = 100/LC5o; TUc = 100/ ICu 4

L '

2) Results of a Ceriodaphnia dubia Chronic / 6 day (3-brood) Toxicity Test (Genus)

(Species) (Type / Duration) Conducted 09/09/98 - 09/15/98 Using Effluent From Outfall 101. (mm/dd/yy) (mm/dd/yy) (number) Percent Surviving Test Solutions . (time intervals used - days) 1 2 3 4* 5 6 7 102 IQQ Control 100 100 IQQ IQQ 0 25% Effluent 100 100 10_Q 100 100 10Q Q 39.2% Effluent 100 100 100 100 100 Igg 49% Effluent 1QQ IQQ IQQ 100 100 100 80% Effluent igg 100 100 100 J0Q igg Q 100% Effluent 100 100 100 100 100 100 Intake 100 90 90 90 90 90 Upstream 1QQ 100 100 100 100 100 i

96-Hour LC50 Value > 100%

Calculated TU Estimates < l.0 TUa 95% Confidence Limits Permit Limit 1.0 TUa Upper Limit NA Lower Limit N_ A Reproduction (# young / female /6-days) Data Test Solutions Replicates 1 1 2 3 4 5 6 7 8 9 10 Mean Control 31 31 29 30 32 29 26 32 28 35 30.3 25% Effluent 30 36 24 35 33 33 31 35 31 34 32.2 39.2% Effluent 35 36 34 33 36 33 34 33 31 40 34.5 2 32.9 49% Emuent 35 29 36 33 31 34 31 36 31 80% EfDuent 30 37 36 33 38 36 35 32 35 33 34.5 100% Effluent 38 29 39 36 36 33 34 23 34 37 33.9 - Intake 0 34 36 31 31 32 31 25 27 29 27.6 Upstream 26 25 32 28 34 22 28 20 25 34 27.4 ICu Value > 100% Calculated TU Estimates i <10 TUc 95% Confidence Limits Permit Limit 1.0 TUc Upper Limit N_A Lower Limit NA ' NOTE: TUa = 100/LCso; TUc = 100/ ICu 8 Ammal died as a result of a leaky cup. i r 5

1 REFERENCE TOXICANT TEST RESULTS (See Appendixes A and D) Species Date Time Duration Toxicant Results (LC30/ICu) P. promelas 08-26-98 1035 7-day Nacl 1837 mg/L (ICu) C. dubia 09-09-98 0900-0915 3-brood Nacl 1174 mg/L (ICu) PHYSICAL / CHEMICAL

SUMMARY

1) Pertinent Chemical Data and Summary of Application

2) Initial and Final Chemistry for Fathead Minnow 7-day and Daphnid 3-brood Chronic Tests See Appendix B

SUMMARY

/ CONCLUSIONS Samples from Outfall 101 exhibited no toxicity to either species tested based on the applicable permit limits.

6

t Appendix A ADDITIONiL TOXICITY TEST INFORMATION

SUMMARY

OF hETHODS

1) Pimephalespromelas Tests were conducted according to EPA /600/4-91/002 (July 1994) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 460 mL plastic tumblers, each containing 250 mL of test solution. [2]

2) Ceriodaphniadubia Tests were conducted according to EPA /600/4-91/002 (July 1994) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30 mL plastic cups, each containing 15 mL of test solution. [2]

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1) Pimephalespromelas

~None

2) Ceriodaphniadubia On day 3,49 percent concentration lost an animal because of a leaky test chamber. The test was considered to be started with 9 animals instead of 10 Synthetic water used as test centrol and dilution water is moderately hard reconstituted water containing trace elements, macronutrients, and vitamins. Synthetic water used for test purposes does ngt contain EDTA. [3]

DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST OROANiSMS

1) Pimephalespromelas None

2) Ceriodaphnia dubia Synthetic water used as culture water is moderately hard reconstituted water containing trace i

elements, macronutrients, and vitamins. Synthetic water used for culture purposes i does contain EDTA. [3] 7

i l PHYSICAL AND CHEMICAL METHODS

1) Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable). All TTL chemicals are recorded in a bound Laboratory Chemical Logbook and specific chemicals used were documented on a chemical record sheet contained in the study folder.

2) Instruments: All identification, service and calibration information retaining to TTL laboratory instruments is contained in bound Laboratory Instmment Logbooks and specific instruments used were documented on an instrument record sheet, along with daily calibration record sheets, contained in the study folder.

3) Temperature was measured using mercury thermometers. The instrument was standardized and inspected with readings made according to TVA procedure ES-42.11. [4]

4) Dissolved oxygen was measured using a YSI Model 57 oxygen meter. The instrument was standardized (using the Winkler method) and readings were taken'according to TVA procedures ES-43.6 and ES-42.4, respectively. [4]

5) The pH was measured using an Orion Model 250 meter equipped with an Orion Ross combination electrode. The instrument was standardized and readings were made according to TVA procedure ES-43.7 and ES-42.8, respectively. [4]

l

6) Conductance was measured using a YSI Model 32 SCT meter. The instmment was standardized and readings were made according to TVA procedures ES-43.3 and ES-42.3, respectively. [4]

7) Alkalinity was measured by titration of 100 mL samples with 0.02 N H SO4 to an endpoint of 4.5 2

according to TVA procedure ES-42.1. [4]

8) Hardness was determined by titration of 50 mL samples with EDTA to a colorimetric endpoint using an indicator (Instructions provided by Reagent Manufacturer [Calgon]), Schwarzenbach Method.

9) Total residual chlorine was determined using the DPD Titrimetric Method according to TVA procedure 6S-42.9. [4]

OUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage; glassware preparation; test organism culturing / acquisition and acclimation; test organism handling during test; and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA /600/4-91/002. [2] Any known deviations were noted during the study and are reported herein. S

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1) Test Type: 7-day chronic tests with hesults expressed as ICu values in mg Nacl /L.

2) Standard Toxicant: Sodium Chloride (Nacl crystalline)

3) Dilution Water Used: Moderately hard reconstituted water

4) Statistics: ICu - EPA Bootstrap program

' REFERENCES

1) NPDES Permit No. TN0005452.

2) Lewis, P. A., D. JJ Klemm, J. M. Lazorchak, T. J. Norberg-King, W. H. Peltier, M. A. Heber. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receivine Waters to Freshwater Organisms. EPA /600/4-91/002 (July 1994).

3) Elendt, B. P., and W. R. Bias. " Trace Nutrient Deficiency in Daphnia Magna Cultured in Standard Medium for Toxicity Testing. Effects of the Optimization of Culture Conditions on Life History Parameters ofD. Magna." Water Resources. Great Britain, Vol. 24, No. 9, pp 1157-1167 (1990).

4) Field Operations Natural Resources Engineerine Procedure Manual. Vol.1, Division of Natural Resource Operations, Tennessee Valley Authority.

I 1 9

Sequoyah Nuclear Plant Biomonitoring September 9-16,1998 Appendix B Pertinent Chemistry Data and Summary of Application Initial and Final Chemistry for Fathead Minnow 7-day and Daphnid 3-brood Chronic Tests

l i i i Table B-1. Analysis of Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Total Residual Chlorine *, September 8-14,1998 1998 Total Residual Month Date Sample Type Chlorine (mg/L) September 8 Grab 0.015 September 9 Grab 0.003 i September 10 Grab 0.014 September 11 Grab 0.013 September 12 Grab < 0.001 September 13 Grab < 0.001 September 14 Grab 0.008

Sodium hypochlorite is utilized in the essential raw cooling water and in the raw cooling water systems at SQN to control the growth of microbiologically induced bacteria and Asiatic Clams. The treatment is necessary for ;dequate cooling water flow, eHicient heat exchange and corrosion prevention. The injected chemical is diluted by the condenser cooling water flow as well as from the flow from other sources.

(sqn0998b. doc)

Table B-2. Water Chemistry Mean Values and Ranges for Fathead Minnow and Daphnid Tests, TTL, Sequoyah Nuclear Plant, September 9-16,1998 Dissolved Omen pli Cond Test / Temp. Initial Final Initial Final Initial Final Alk. liardness TRC Jample ID ( C) (mg/L) (mg/L) S.U. S.U. (pmhos) (pmhos) t Fathead / 25.1 8.4 5.5 8.2 7.6 334 349 69.0 88.0 Control (24.9-25.3) (8.3-8.4) (4.9-6.5) (8.2-8.3) (7.5-7.8) (331-341) (344-354) (69-69) (88-88) Fathead / 24.9 8.2 5.3 8.1 7.6 301 308 101-25 % (24.7-25.2) (8.0-8.4) (4.4-6.4) (8.1-8.2) (7.5-7.7) (297-307) (305-312) Fathead / 25.1 8.1 5.3 8.1 7.6 276 285 101-39.2 % (24.9-25.4) (7.9-8.2) (4.4-6.2) (8.1-8.1) (7.5-7.8) (275-277) (282-289) Fathead / 25.1 8.0 5.2 8.0 7.6 260 267 101-49 % (24.9-25.3) (7.8-8.1) (4.8-6.1) (8.0-8.1) (7.5-7.7) (260-261) (266-269) Fathead / 25.1 7.9 5.4 7.9 7.6 210 218 101-80 % (24.9-25.2) (7.6-8.1) (4.8-6.1) (7.9-8.0) (7.5-7.8) (209-212) (217-220) Fathead / 25.1 7.7 5.4 7.9 7.6 177 184 62.7 68.9 <0.1 101-100 % (25.0-25.2) (7.4-8.1) (4.5-6.2) (7.8-7.9) (7.4-7.S) (175-179) (183-188) (62-64) (68-70) (<0.1-<0.1) Fathead / 25.1 7.1 5.4 7.7 7.5 177 182 62.1 68.6 <0.1 Intake (24.9-25.4) (6.6-7.9) (4.3-6.9) (7.6-7.8) (7.4-7.7) (175-178) (180-185) (61-63) (66-70) (<0.1 -<0.1) Fathead / 24.9 7.4 5.3 7.8 7.5 177 181 61.7 68.3 <0.1 Upstream (24.7-25.1) (7.2-7.7) (4.8-6.0) (7.7-7.8) (7.4-7.8) (175-178) (180-185) (61-63) (68-70) (<0.1 <0.1) Daphnid/ 24.8 8.3 7.7 8.2 8.2 328 64.0 87.0 Control (24.1-25.5) (8.2-8.3) (7.6-7.8) (8.2-8.2) (8.1 -8.2) (320-333) (63-65) (86-88) Daphnid/ 24.8 8.2 7.6 8.1 8.1 289 101-25 % (24.1-25.5) (8.0-8.3) (7.5-7.8) (8.1-8.2) (8.1-8.1) (286-294) Daphnid/ 24.8 8.1 7.7 8.1 8.1 268 101-39.2 % (24.1-25.5) (7.9-8.2) (7.5-7.8) (8.0-8.1) (8.1-8.1) (265-272) Daphnid/ 24.8 8.0 7.7 8.0 8.1 253 101-49 % (24.1-25.5) (7.8-8.1) (7.5-7.8) (8.0-8.1) (8.1-8.1) (249-258) Daphnid/ 24.8 7.9 7.7 7.9 8.1 204 101-80 % (24.1-25.5) (7.6-8.0) (7.5-7.8) (7.9-8.0) (8.1-8.1) (197-207) Daphnid/ 24.8 7.7 7.7 7.9 8.1 177 62.8 69.0 <0.1 101-100 % (24.1-25.5) (7.4-8.1) (7.5-7.8) (7.8-7.9) (8.1-8.1) (175-179) (62-64) (68-70) (<0.1 -<0.1 ) Daphnid/ 24.8 7.2 7.7 7.7 8.1 177 62.2 68.7 <0.1 Intake (24.1-25.5) (6.6-7.9) (7.5-7.8) (7.6-7.8) (8.1-8.2) (175-178) (61-63) (66-70) (<0.1 <0.1) Daphnid/ 24.8 7.5 7.7 7.8 8.1 177 61.7 68.3 <0.1 Upstream (24.1-25.5) (7.2-7.7) (7.5-7.8) (7.7-7.8) (8.1-8.1) (175-178) (61-63) (68-70) (<0.1 <0.1) mg/L CACO o 3 iTotal Residual Chlorine

Sequoyah Nuclear Plant Biomonitoring September 9-16,1998 .a Appendix C Chain of Custody Record and Toxicity Test Bench Sheets

0-PI-ENV-000-017.S SQN - C//foo,c NPDES AGUTE TOXICITY MONITORING R:v. 0 0 0r5 Page 7 of 7 T-t-12 APPENDIX A Page 1 of 1 ein orks WS -AGUTETOXICITY CHAIN OF CUSTODY RECORD Sequoyth Nuclear Plant PROJECT NAME:, SON NPDES TOXICITY COLLECTOR (SIGNATURE): 6, Custody 3 Rt blJ; ^ ' TRCJij COLLECTION COLLECTION FIELD 10 SAMPLE TYPE

NOL esyTTL@n START FINISH DESCRIPTION SAMPLES h!l.AB[$j ; EMR QC)gf Ekays @!!Sisispr NUMsha

$3I #mg 3iNM !s!MA$ bel DATE/ TIME DATE/ TIME DSN101 Grab 2.5 gal gyg ggg.;gg ggg;g gggg lf8M 0931 g/RhA o931 %qlB-D50 lot 'a'aka o'ab aa' MM2 m iig' 8 'Mi'88s s ki n W Wenan% 9Wd%% seeumys Upstream TR (486.4) Grab 1 gal gg gjpggg gj' g cygggj { 1, h5 0951 9l0hP3 o 95') h AlS-op6TREi\\M h SAMPLE FLOW (MGD) DSN101 Shipping Method: Fa Le-RELINQUISHED DATE TIME RECEIVED BY:(SIGNATURE) DATE TIME BY:(SIGNATURE) Y J fj ), &YS llI/0 llELINQUISHED ' DATE TIME RECElyeD BY:(SIGNATURE) DATE TIME BY:(SIGNATURE) REMARKS:

1 SQN-0-PI-ENV-000-017.S I NPDES ACUTE TOXICITY MONITORING Rtv. O ~ O Page 7 of 7 APPENDIX A Page 1 of 1 ein aFle ACUTE TOXICITY CHAIN OF CUSTODY RECORD SIqu yah Nuclear Plant PROJECT NAME:, SON NPDES TOXICITY COLLE OR (SIGNATURE):

/

COLLECTION COLLECTION FIELD ID SAMPLE TYPE

NOL MTTI{

j;MRRlVbl#g pg Cjg @gCtisted & l#sels

START FINISH DESCRIPTION SAMPLES qLABI

)glEMf3ggc)h QM ty DATE/ TIME DATEITIME EC9 Min di F $lial ihSit ETn@lt li 9fl5 08fY 9hhB 0851 SGd 919-B5k31ot SN101 Grab 2.5 Dal gy{gyg igg g g gggy yg{gggg gggjgg 9hhB o#

9hha o'*1 Seu 919-iuTue ' "'" k" 8 *' UM^iM W i e Bli! *E^A#UiM&M 9hl9B 090T 95l98 Oil EQu9h-OPSTRERM Upstream TR (486.4) Grab 1 gal gjyg; ^{ygggM* Fgjgy

ggg g

l SAMPLE FLOW (MGD) DSN101 Shipping Method: [EOggl B(((E55 - Gy o s t s-111 RELINQUISHED DATE TIME RECEIVED BY:(SIGNATURE) DATETim* TIME Dc6 BY: S NATURE) 9/wf3

0 f&

LU sw a>-alid m sbbs RELINQUISHED DATE TIME RECElyED BYi(SIGNATURE) DATE TIME BY:(SIGNATURE) REMARKS: lb)El\\Y0EA: Juan) CML + wJOJ A;fL YEM P M C-u A Yet 'thint. up McAu nNO.fMTAkc y -er Dsp jot rru n. 3 5 ' c' ' u n~rt A - cl.knk nHD 'c8sbad yd i il l 9.T-TT f 1 l

0-PI-ENV-000-017,S SQt4 NPDES ACUTE TCXICITY MONITORING Rav. 0 Page 7 of 7 0 APPENDIX A Page 1 of 1 e A al@ -AGUTE TOXICIT( CHAIN OF CUSTODY RECORD 5equ yih Nuclear Plant 3ROJECT NAME:. SON NPDES TOXICITY SOLLEC (SIGNATURE): og ud _. ~_.f a ~ Custody! COLLGCTION COLLEdTION FIELD ID SAMPLE TYPE

NOL NTTL g(AR

$l $ly START FINISH DESCRIPTION SAMPLES hl.AB ATM lE Fiht-L 2 W 2 DATE/ TIME DATE/ TIME DSN101 Grab 2.5 gal j;gg ggggg g gg j;gp;;gg ((l6@ oS15' 9flBM 085Y SDM ql16- (MM(ol Inta b Grab 1 gal gyjylggg j{gfggggggg{g gggg gggggggg ifl6% o 8o4 lltohB 081' SQu 911b-NrAKE "E ( ^} U"' NfM)b hYkbf$bdb [hb2$h $$[bbb lflOM 09lo l16I18 D9 f6 SQL54IID-OPSTREAfv\\ 2 AMPLE FLOW (MGD) DSN101 Shlpping Method: fgg7gyL Ex p(zySS i RELINQUISHED DATE TIME RECEIVED BY:(SIGNATURE) DATE TIME BY:(S GNATURE) 9-ll-9 2 0945 A &) qllona jooo RELINQUISHED DATE TIME ' RECEIVED BY:(SIGNATURE) DATE TIME BY:(SIGNATURE) i al, MA cob w a rrA T4,A. O,m - lo l V/ C %3rp)(r & M ITTE S REMARKS: A. A wo: 11 ~ <- wsATilE 6 : Judd c REM D. 26"t w'm A: c.L b A + c4 i

0-PI-ENV-000-017.S SQN NPDES ACUTE TOXICITY MONITORING R:.v. 0 Page 7 of 7 0 APPENDIX A Page 1 of 1 ch a44.914B -AGUTE TOXICITY CHAIN OF CUSTODY RECORD Aqu yah Nuclear Plant PROJECT NAME:, SON NPDES TOXICITY COLLyOR (SIGNATURE): Y gCjk AC4stod COLLECTION COLLECTION " FIELD ID SAMPLE TYPE

NOL

pTTl;g fARRl val;@)ji siffMaD[%is 93} gym $$ssis,y sid NAB lfi.D BTEMP QC Ji% i!j t

START FINISH DESCRIPTION SAMPLES astiSit M86PJZ DATE/ TIME DATE/ TIME U"' N$dhb5 $.$I !$85 b$!hh bd$k[sE3 lllM dB2i G/llI?A 803# Od 9l11-D$4161 pgg; ;j g ylgj[gggggg in ab Grab 1 gal ggglp \\ 9lflh 0?FS-9)lll9B n')f 9 Mk) 9$11 - IMTAKE ab 1 gal g pg ~ gp]p< gg ggj G 9/l1l98 o36 9l l98 08fl SQo Alli-Up5TREM /p/ /j&/ "I SAMPLE FLOW (MGD) / -tv (Je L uSe DSN101 ga ShippinD Method: Fict 6 r, o 9 RELINQUISHED DATE TIME REC IVED BY:(SIGNATURE) DATE TIME BY:(SIGNATURE) hl " VN~S 0?V Qgg qq,g jooO RELINQUISHED tl DATE TIME RECEIVED BY:(SIGNATURE) DATE TIME BY:(SIGNATURE) l 95 ' I)3fd~Iol 3 7 "C werd cLEAA ItHO snsarfl. REUARKS: ta nYfli Terp; UAfrAEAP t DYA h LuGWfAEA: 6:fL 'fo1,4 ' Pt *C IM /lYL4 CL w l) p od cpLp

SQN. 0-PI-ENV-000-017.S NPDES ACUTE TOXICITY MONITORING R;v. 0 0 Page 7 of 7 APPENDIX A Page 1 of 1 clawb. 6 8 -AGUTE TOXICITY CHAIN OF CUSTODY RECORD Mqu y:h Nuclear Plant PROJECT NAME:, SON NPDES TOXICITY COLLEC (SIGNATURE): & lx] COLLECTION COLLECTION l FIELD ID SAMPLE T(PE

/VOL

iiTTli p

R1 VAll% BT i;j ticustod a IISB%gg START FINISH 9/l 8 DESCRIPTION SAMPLES Ek OjS @d : J $$dssis.! I DATE/ TIME DATE/ TIME B

dd#iiWie

Mb ?!@k 7" s2 $$$hTJ IflAhA 0735 9fla m d B M SG*a 9lla-D5olot DS m Grab u gal ggjgyj lgy,jpggigp] g3jgg jggggggj lll2NR OWS 9lIAIB O'l'I7

%3 9 IIA-1MTAKE U"' INNfbI b b!d !bbMS N$kkhbEN Ifl2hA O TNO 9ff4$8 094S SGM4lla-UPSTRE AM bkbb h Skhh b b dh hb. SAMPLE FLOW (MGD) DSN101 Shipping Method: that CmJLAw ( prh CC) RELINQUISHED DATE TIME RECEIVED BY:(SIGNATURE) DATE TIME BY: GNATURE) 7 b 04-i 2 d T /5# MA) qliahs //W t-RELINQUISHED g DATE TIME RECEIVED BY:(SIGNATURE) DATE TIME BY:(SIGNATURE) wRty faut <r Tem o = 3 9 C cu //Vis REMARKS: for. I mer Tr# (tdStren^ I ll,C, ra n rr A 7txo Av $,rrarc A3 ta hir R ; c l.rA tt ' + <- nl A W Ek v$t h C. LEA A dvh] IWO tooG yen 0 / $ ' C. I

SQN 0-PI-ENV-000-017.S NPDES ACUTE TOXICITY MONITORING R;v. 0 0 Page 7 of 7 APPENDIX A Page 1 of 1 op wh $he -AGUTE-TOXICITY CHAIN OF CUSTODY RECORD pequoyah Nuclear Plant fROJECT NAME: SON' NPDES TOXICITY COLLEC (SIGNATURE): & lA E ocu~v-l FIELD ID SAMPLE T(PE

NOL JTI!Q iMRRIVAL;(fl dCustody)

.gTi1Q6 COLLE'CTION COLLEdTION

Sblitss~

START FINISH DESCRIPTION SAMPLES

!LABi#lr firEhig'~~("'C)

DATE/ TIME DATE/ TIME s 0' N5 > $NbiSJE Eddi WE lfl3 $ 033A 9ll3hb OF3F % 4l13-D5Miol '"'"k" U'"" ' 0 "' !MM !dOWi3 ~ BP inha lit #B o 4 Ali3h8 o 141 5(N All3-inTME 0 $hNUI$ $d9 d$QL Mi5 ll13% 07M Rfl3IlB ODE $091I$r UpsrRE AM D759-/VM SAMPLE FLOW (MGD) DSN101 Shipping Method: Mg h (pr a h) RELINQUISHED DATE TIME RECEIVED BY:(SIGNATURE) DATE TIME XY:(S 1ATURE) MM qlghg //dd bb"7 RELINQUISHED / [] DATE TIME RECEIVED BY:(SIGNATURE) DATE TIME BY:(SIGNATURE) REMARKS: rpATf4 Y610 DF for JT C W TAkt 2.5 ' t. a f Jr/ FAr. 34 C VFL A ". C.bfM fDJg CA h ^* 'A :R yep P-17*t ca EnYILK S W I AHO 't-EAA I W enn ay pa s->y 7 y l

0-PI-ENV-000-017.S SQN NPDES ACUTE TOXICITY MONITORING R:;v. 0 0 Page 7 of 7 APPENDIX A Page 1 of 1 e uh448 -AGUTE-TOXICITY CHAIN OF CUSTODY RECORD Sequ;y::h Nuclear Plant . ROJECT NAME:. P l SON NPDES TOXICITY COLLE OR (SIGNATURE): artnj $ h. Wnm f} hRCg stodpj i d7#g jsTEd$[^Ih DANOI @lh;TTLph COLLECTION ~ COLLECTION U FIELD ID SAMPLE TYPE

NOL g

(5l5d START 4 , FINISH DESCRIPTION SAMPLES g P&AWs S$$$Ma in ZECi? 3 V3EBi DATE/TIMET DATEITIME 0*' NNNY ishN[$N k!b Ndkb NkI N hbfM 9llih6 OMl. Mo 9Il4-b9J 101 int ko Grab 1 gal sg/gjg g3ggg 8g:gy tgg{ Eh4M 01sf 4h419e o'13'1 soeb+- imme Upmeani m @86 A) Grab 1 gal gypg, g gyjgggg gggg ppgggg ' h4h8 o 6 R 3 9h4M8 o3A3 20%- Uosterms R l SAMPLE FLOW (MGD) IDSN101 l Shipping Method: fEb-EX l RELINQUISHED DATE TIME RECEIVED BY:(SIGNATURE) DATE TIME BY: NATURE) Qh-qli4[qg /000 - / _,'c6 Nre &o REllNQUISHED i DATE TIME RECflVED BY:(SIGNATURE) DATE TIME BY:(SIGNATURE) l M c Os & lol 39 *C wAra: et a < + c.A a REMAR S: id ATE /L MA : T&DNE 25'C M WAMrs U En1 EA ; A;A YrM,0l 12 'C 6 twJ ti; eALM AND G-Co A.

CHRONIC STUDY RECORD SHEET Study: SON Outfall 101 Chronic Test Organism: P. promelas / C dubia Initiation Date/ Time:09-OhD!/336 / 09 ff & -/377 Termination Date/ rime: 09 //,-9f,!/ 2 36 / rA-is -H / lh - l'l10 Control / Dilution Water: F-medium: 6 loa / Cerio medium without EDTA: 2RL ; 2 7(36 Personnel: TT3 D u/ der. MC Cw / / J Test Treatment Site: Concentrations: Outfall 101 25.0 %.39.2 %.49.0 %.80 0 %. 100.0 % Intake 100 0 % Upstream 100.0 % Site: Outfall 101 Intake Upstream D_n Date Loe #'s Loe #'s Loc #'s , Loc #'s Q 99 19 ]li$ }{ 14 3I10 J, 04Pii Alf4 3tti S tic 2 05-(t-it 3191 3tsi s t.5 3 } Obit 31 3Kt S d;t 3(53 4_ W r3 si 3tif 31 4 b g.n if } ci-t+1f 32ot 31o t 52o3 6 65-si 13 3?oi Sue 3 74 \\ P. oromelas: Spawn Date Tile # Hatch Daterrime to Datc/ Time W 9-$'11//To. 9 Ts/I 2 of C dubia: Released From: c5 di s1/2 loo to o ( o<-e / oceo >f o "l-P. promelas: 96 hour LCp: > loo '/. IC:5:- C dubia 96 hour LC o:

' o * '/-

IC:3:; > ' o o */. 5 ~ jy 'i-9 9 % ) c g.49 pf Iibo 814C b. Ii hhn f 9-4 4% note. q

e. e, at 11in

I i SON DSN101 Dose Gesponse Cuce i i Test Dose Response Curves SON DSN 101 Septameer 9,1996 Peh Growth Fan % Survus % Emuent Response Smoothed % Emuent Resoonse smoctoed 0 0.b94 0 649 0 96 IwA 25 0 634 0 649 2S 94 39.2 0.630 0 64 39 2 92 49 0 691 0 649 49 100 80 0 697 0.649 80 to 100 0 637 0637 100 97 Sequoyahhp,03 4101, Fathead Growth Sequeyah NP, DSN101, Fathead % Survival

- $.'...'T..*....Q.....^..........'.:.4

0. tow - * -. -... - - =.

=x-s0 00 y ...................---. g r~ I g 0.so < g er n............................... 0 30.............................. 4 78 " ' ' "..."...".....".."...."..."....."...*4 so 00 - 4 to 50m 0 25 39 3 40 to 100 0 25 39 2 49 00 100 Ef%ese Censeseamen grese4 Ceaseousen Daphnid Reproducton % Emuent Response Smoofr.ed 0 30 3 33.1 2S 32.2 33.1 39 2 34.5 33.1 49 32.9 33 1 80 24 5 33 1 100 33.9 33.1 Sequoyah NP, DSN101, Daphnid Reproduetten de smoseed usare t I 3S.-. -:-*. n u -- =--- - - --.4 'b 20T M T.....~........ I2s. ...................... ~.... te.,............................. 15 0 25 '.3 3 de 30 100 f fbese ceas esennen l l i 1 1 l i 4 Page 1 of 1

l FATIIEAD MINNOW LARVAL SURVIVAL AND GROWTII TEST Page 1 of 2 Test ID: SON Chronic Pans: Date/ Time of drying: Ulh $$E Fish: Date/ Time of drying: din oYI Initiation Date: f-( (P Time: 03o Pans: Date/ Time of weiglung: 9-nt 97 y5 Fish: Date/ Time of weighing: 1-l I 9 8 #dh l 7 Termination Date: 09_/MP Time: /130 Sample ID/ Rep.

Alive Tare Tare + Fish i

Concentration y Weight Weight I - Notes: Day 1 2 3 4 5 6 7 g c i Control Log # o/od O/00 4 /OC O/ D o C /0 0 0/00 g ra e (F-medium) 1 /D IO io /0 )) 10 0,93I %

0. 9 s (,4 (,

2 /D 10 /d /c to /o /o 0.97914-0,97495 3 /4 lo /d eo /0 to lo 0.974 19 0.99132_ 4 /4 10 /0 4 W T S 0.97387 0.98991 By (TV}( 300 (rEE Sid 'X0-TLC- ~Jd C-UN-Outfall 101 Log # N/A N/A N/A N/A N/A N/A N/A 25.0 % 1 /o lo /0 /n /0 /0 /o o.97860 0.98559 'o /0 to lo C.97815 0.95400 2 la l0 /0 / 3 /4 9 C/ 0 9 '9 9 0.97313

0. 9 74-M 4

/0 lo /d /0 lo /0 /0 0.97565 0,97133 By (rVK 3CC Crkle' JCC C5 Toc-XC-7' Outfall 101 Log # N/A N/A N/A N/A N/A N/A N/A l 9 9 9 0.97727 0.99455 39.2 % 1 /B /O /0 4 2 /0 to /4 /0 lo (a lo 0.9 s s i 4-o.99051 q #, y y a 3 9 2 2' W '1 7 7 0.9 2 Ms 0.99095 i

y. d : W 4

/0 10 /4 to IO /d lo 0.9846/ o.9910I Y By (M/// 3CC. (rff/ 0 55 .~k"C ETS 36C-Jcc. Tsd. Outfall 101 Log # 'N/A N/A N/A N/A N/A N/A N/A 49.0 % 1 ll 10 //) to /o f.o lo

n. 9 9 0 71.

0.98 7 M 2 /0 lo /0 /0 /0 /0 lo 0.9F5 l 9 0.99233 3 /0 /0 /O to (0 to to 0.97495 0.99140 4 /C /0 /6 (D l0 to to o,9 7 9 3(o 0.99434 By (rh SCC CrK/l 1725 %CC ZYJJ Tet- ~ M~~- TdC Outfall 101 Log # N/A N/A N/A N/A N/A N/A N/A 30.0 % 1 /o 9 9 4 9 9 9 o.9Sco9 o.99247' 2 /o IU /d to /0 lo Id 0.98597

0. 992 fol 3

/0 lb /C le /o lo /0

0. 9 K 77 3 0.9952.7 4

to /c /d to ts to 10 0,9 90 g 9 o.99 g 11 By ICC. Cr//f (4/( C.us .2175 JCC. Cf/(__ ~Tdc. 7dC ' l Outfall 101 Log # 3;M 2 / 9c/ 3(4/ 1/4/ 3/4? 3to( 32o9 l l 100.0 % 1 /0 /0 /C to /, /0 /0 0.99I93 0.9 9 7 1 /o deM W.'L,I 3 to /o cr#irg"_ 10 to lo /d 0 97S93 n,97 5 73 M A M f. s 2 /0 /0 /C 9 9 9 4 c.9794o o954S5 NM"8 4 to /0 /0 to 9 -< 9 9 0.97717 0 92247 By JCc Cf/L Crk/C h &O

~CC (AY 7dC M

Intake f Log # 3l?4 Wlf86 % 3 41 jf 42 7 f f1 3 rs v 32/o 100.0 % 1 /0 M /6 /6 la to 10 /0 097699 0.9 8 Vl f 2 /0 @ /0 , /6 to /= to /d 0.9344l 0.99 l 2 O 3 to /v 4.7:;"9 to 9 9 9 9 o.9 % n

o. 99 x f 4

/o /0 /6 to to lo // 0,98747 0,99 3M By QCC (M. 6 tf/?s) Cw Cfi) -TCC [r3X 36 C- ~}dC 0 1 2 3 6'tde 4 5 6 7 Fed-Time /By: LlA

t. ( A l - 1 9 ls1C opos/m Cis*Wo&~% J W lo'5

-ll AlA /n30lfrM. MS b Lh 107/lG% Wo!' l043&L /dtgE2. l 1351lan H/Yk%I-- @bec t@/Ed Ha/D6 I 9'<1!v D (b7/.WC. O Renewal Time: X MO l337 l3 N 9 b O n '9e/W $mf-G/* /N O Dilution Water Otoo 6 foo blo 0 01 o o em o/co omo olo O Reviewed Br M L Nb b 3N N . O:N A

FATHEAD MINNOW LARVAL SURVIVAL AND GROWTII TEST Page 2 of 2 Test ID: SON Chronic Pans: Date/ Time of drying: h: Date/ Time of drying: r Initiation Date: 1-1-11 Time: /330 Pans: Date/ Time of weighing: VC 'I: te/T Termination Date: 69 /4. fP_ Time:f.3_3 g d, C - Y l'T {_c of s fghingl P Sample ID/ Rep.

Alive Tare Tare + Fish Concentration Weight Weight Notes:

Day 1 2 3 1 4 5 6 7 g g Upstream Log # 3I80 3 fflo 3f49 SI 13 52#

20 ')

3211 1 /o /6 <O lo la /0 /0 0,977,57 0.99225 2 /D /0 / O 10 /n /0 /4 0,9 31(, j 39931 3 /0 /o lo 10 to /0 /d 0.97179 o,97944 4 /0 /6 <0 I0 to /0 /d 0, 9 g (, 94 o,9 9 37'i By ZC_ Gff DD U" CU5

dC-(JJ To c -

7de_. Log # 1 2 3 4 By Log # I a: 2 3 4 By Loc # i 1 2 1 ! 3 4 l l By i Log # 1 2 3 By Log # 1 2 3 4 By 1 Loc # 1 l, 2 3 f 4 l By l l 0 1 2 3 4 5 6 7 Fed-Time /By: / / t / l 71. OIP/4 /) i 7, Renewal Time: X / I\\ E L

b 8

Dilution Water 0:oo otoo otoo oron otoo_ Oteo em Reviewed By: _b_Lk Oyh C-O(. d3 bl p MR tr (# g

~..... _. -..~.- ~ -. ~, _ _ - - _, _ - - -. _ _,. _ _ _ ~ _ ~. ~... - - n_.. k 8NQ 8est, Isge s FATHEAD DftY WEIGrrit.ECDRD SHEET PersonneLICC Study / Dele.5QN Outfall 10109M96 Pens. Data / rune et Dryvv 9-13 9&O943 FADase/ Tuns of DryngC9-l6 98/1313 o 09149WOS23 g 0917 9g0310 Pens Dela/ rune ofweshang oWi&98/10051033 FADeuwTune ofWeghug0Wl7-9&O9lSO940 sarnpu ID Tars WL Tare + Fuh WL Fe WL L....,8 Fah Mean WL Surwving Mean WL Totas Wt IC23 WL LC23 Mean WL Surwm Conrot e t 8 e mg m8 me mt Mean Wt (mt) O V8150 0 98640 0 00496 10 0 000496 0.4%0 / e.4960 0 49e0 Connot 2 0 77924 0 98495 0 00571 10 0 000571 0 5710 23 2623 eJ710 0J710 0 3937 i 3 0.98429 0 99132 0 00703 10 0 000703 0.7030 0.7030 0 5937 0 7030 d 4 0 98387 0 98W2 0 00605 8 0 0007163 0 7563 a/ e 6050 0 6050 I 0 9'1160 0 98559 0 00699 10 0 000699 06MO e 6*90 Qg k# I CatfaG 1017 0 9'825 0 9E400 0 00575 to 0 000575 03750 26 0200 e.5750 i 23 0 % 3 0 97813 0 98417 0 00594 9 0 00066 0 6600 43940 6340 d 4 0 97965 0 96233 0 00668 10 0 000 % 8 0 6Mio 0.6630 41 l 0 98827 0 99458 0 00631 9 0 0007011 0 7011 0.7 ell h W" Catfall 1012 0 98314 0 99051 0 00737 10 0 000737 0.7370 27 0668 0.7370 p3 b 39 2 % 3 0 911655 0 99095 0 00440 7 0 000o286 0 6236 e.4400 0 6295 2 4 0 98461 0 99101 0 00640 10 0 00064 0 6400 0 6400 V I 0 98072 0 98765 0 00693 to 0 000693 0 6930 9.69 le OutlWi l012 0 98519 0 9923s 0 00719 to 0 000719 0 7190 27 6500 9.719e f 490% 3 0 984a5 0 99140 0 00655 10 0 000e55 0 6550 0.6550 0 6912 2 4 0 98736 0 99434 0 00608 10 0 000e98 0 6980 0 6900 1 0 98609 0 9V248 0 00039 9 0 00071 0 7100 0.6390 Ourfall 1012 0 98587 0 99261 0 00674 10 0 000674 0 6740 28 6100 0.6744 OO% 3 0 98773 0 99527 0 m3754 10 0 000754 0 7540 0.7540 0 6975 t 4 0969 0 99812 0 00723 to 0 000723 0 7230 0.72Je 1 1 0 99193 0 99tf?6 0 00633 10 0 000033 0 6330 06330 Cattall 1012 0 97893 0 98573 0 00080 to 0 00008 0 anoo 26 1744 0.6800 100.0 % 3 0 97840 0 984H5 0 00045 9 0 0007167 0 7167 e.6450 0 6364 4 0 97718 0 98247 0 00529 9 0 090Nr's 0581 0.5878 1 0 97699 0 98378 0 00679 10 0 000679 0 6790 e 6796 intaxe 2 0 98441 0 99120 0 00679 to 0 000679 0.6790 2.5 7467 0.6790 3 0 98686 0 97241 0 00535 9 0 0006167 0 6167 43550 0 6283 4 0 98747 0 99347 0 00n00 10 0 0006 0 6000 e.6000 1 0 96657 0 97225 0 00508 10 0 0005es 0 5080 0 5640 Upstream 2 098261 0 9u932 0.00671 10 0 000671 0 6710 13 9700 0.6710 3 0 9lr2M 0 98944 0 00065 to 0 000065 0 6610 6.6650 0 6492 4 0 98084 0 99377 0 00093 10 0 000093 0 6930 0 6930 Asnewe6 Sy Itaued

9. w -R Ps9e I of 1

6G sJ y 4, s itt F.% ~Jcps Cn os so-T1 000 LISTING OF GROUP CONCENTRATIONS ( r. EFF,') AND RESPONSE MEANS *** CONC. (%EFF) RESPONSE MEAN MEAN AFTER POOLING .000' .594 .649 2S.000 .634 .649 39.200 .630 .649 49.000 .691 .649 80.000 .697 .649 100.000 .637 .637

- - NO LINEAR INTERPOLATION ESTIMATE CAN BE CALCULATED FROM THE INPUT DATA, SINCE NONE OF THE (POSSIBLY POOLED) GROUP RESPONSE MEANS WERE-LESS THAN 7S.0% OF THE CONTROL RESPONSE MEAN.

80.000 .697 .644 100.000 .637 .637 cc* NO LINEAR INTERPOLATION ESTIMATE CAN BE CALCULATED FROM THE INPUT DATA. SINCE NONE OF THE ( POSSIBLY POOLED ) GROUP RESPONSE MEANS WERE LESS THAN 75.0% OF THE CONTROL RESPONSE MEAN. c*********************************************************** c BOOTSTRAP PROCEDURE TO ESTIMATE VARIABILITY c OF THE ESTIMATED ICp cc****************************************************xar*** 000 BOOTSTRAP ESTIMATES OF ICp FOR ALL RESAMPLES WERE ABOVE THE HIGHEST CONCENTRATION / % EFF. BOOTSTRP)

l CERIODAPHNIA SURVIVAL AND REPRODUCTION TEST hir !ONChrmie has 1 d 2 i sco Dae: 9-9-f F Time: I J la- /JJi Termmm2cn Date: bII i E Tb /32.u-148o l I ; ment By-G W 67) DJ.5 F MW Total shdo zation #) Rep 1 2 3.- 4 5 6 7 8 Young l ^ Legs j ql, g, ]'H 2 Lkt 27(,2-- 274ti a'%5 1 O n n 4- /l /fo sf 7 f l (o /t /4

rl 3

4 /( I4 acy ccoor(i) 4 g- // /4 23 1 l (Caedium w/o EDTA) 5 ( /Z [S' qJ, f 6 s~ l2 - / Z --- cai 4 9 LS 26 \\ 8 T Iv /r 2;k. 9 i g"- /0 - l3 p,{ i lo S~ O (Z ' - ([( 3f 303 ~ Les8 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A j 1 C) o a 6, /4 /C 30 2 I (, L3 /7 3(o 3 A /I /c

2.4 omrauici 4

i ( r /1-. /( 3( I 25 o * (2) 5 l q- 't/ /g' q3 l 6 T ll l7 gg l + (Z - /C Al i G /2 -- / 7, gr l 9 ) '/ /t %t l 10 f b 5-o // /T M. ln I Los8 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A l l D O O T (3 17 RC l 2 I 6 IA /f %/o } 3 i f /2 - // 34 l omrau ici 4 5- /z - /(, a3 i 39 2 % (3) 5 [O /2 - /I 3[o i 6 (n (2 - /S' AA 7 y /Z- /f[ RQ 8 h S- /t /7 s4 l 9 /./ O Y /f 11 1, /, o /t 2o 40 C D-to e les8 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 1 O O 4 / 2 17 n 2 1 S~ W /6 2.9 l 3 (o I2 '" /f Els ouuau tot 4 l /0 /f( 3A 49 w. (4) 5 _f //. /5' 2I l 6 6 // /~/ q q., 'f- /* t ~l 3I s G /7 it, e 9 3 C II /7 'RI .LY .$10 (W L LAeroMuo nts o.o *o e rro S '? hn rMM v ^ q8, 0 1 2 1 e i s 2 8 che rDn: 27 f 4 7-/ 22.L M g7 37-2. - r1s2aton: Iat..1. IeS-3 IGkL IacL Ice 2 ht, - nama wacr ids: 23,(1_ dDZ"1kb. 7hE [Ed" -2E.r T e h: M Time-194 Iba (32E. Ist( Ltto 13 2o e -*llo v.,cee %.[ g D& f u.viewca or Approved er

CERIODAPHNIA SURVIVAL AND REPRODUCTION TEST Study: SON Chrmic Page 3 of 3 inaiaum Dae f-9 If Time: 1320 -I S V Terummam Dze-h~l6" Time _ l'$ 20 -(M/D Treatment By: 7 W 1)'T J OF M D S-Total (Randomization #) Rep i 2 3. 4 5 6 7 8 Young Los8 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 1 O n to (p /2-(L 30 2 1 t (o f3 /g 37 3 6 L9 /7 o esnioi 4 36 r // /7 8o 0%(5) 3 gg (p ff i7 \\ 6 y( i f /4 '/ Y 7 zg C // /f 8 Ar + tl /7 11 s* 14 /C, 2< 10 'l i s' (a o il /& 2, 2, 3W Legn 3lqgf J/gy 3qqf qqgl ff$g gyl 1 o.iHM^o O G /Z - zo N( 2 ~ (s 4 /9 M 3 (o 13 zo R9 odsu tot 4 f,, /2 - l8 .hlo ico o*(6) 5 i c /2_ fr 3(o 6 A t/ /6 33 7 g if b fO 20 f& 8 i OM C G> /L - 22, 9 / _ n a. // /'7 34 to t! J/ S o / 2_-- z.o 37 g Lago )Iqq f 3r15 u f 2. $ t42s fi66 3SD 2 ' I O D(f 3 0/n m /3 2 I O 13 [ t( /7 y 3 1 (o o L3 /7 3l, Intake (7) 4 0 7 /O /b 3[ 5 .r-o (I /7 3I 6 o f /1 /S 3A 7 g) 4/. /1 ($" 3l u (o o r7 G> 2.T v \\ O 4- /0 13 27 io 4 i # er o 9 /6 29 17k Los s 3;e o +1 s i rt, s#f7 3 13 3aeo 3a0_3 i oA4G e9 o 5-V 42. 26 2 g. /3 6 25' 3 Y /.3 /8 31 ty m am ts) 4 (o /0 // - M S (o II /7 34 6 .s-- ( 2. - 5-1,, 7 y 9 (5 ?J( c rz.- g 2P x v I O /0 /V e'L5~ to i O (o o il (7 3+ T'4 O 1 2 2 a 1 6 I I Chow ids: I Alase !D*: { <1 l _d_ dd OM b 1 Du,m w,c, cD. 1** Tirue: venne: } i g.Di(W W1 (gy wa ar ApprovedDy % V luk.1;.,u, oule usa,,l,,w;. gyC I 9 As J slw a 1 sir. A la (d), lat % lre s+<ess*J Mh lH-4 d. Mm - ins rw%wJae

4 iTHELNUMBER OF RESAMPLES IS 80 6Q0 M Ne IDI OO -TC Cx oT-87 U -c** LISTING OF-GROUP CONCENTRATIONS '( *4 EFF.) AND RESPONSE MEANS *** CONC. ( %EFF ). ~ RESPONSE MEAN MEAN AFTER POOLING .000, 30.300-33.051 -1,

25.000 32.2001 33.051 39.200 34.500 33.051 2 49.000.

32.889 33.051 80.000 34.500 33.051 100.000-33.900 33.051' coa NO LINEAR INTERPOLATION ESTIMATE CAN BE CALCULATED FROM THE INPUT DATA, SINCE NONE OF ' THE (POSSIBLY POOLED ) GROUP RESPONSE MEANS ~WERE LESS THAN 75.0% OF-THE CONTROL RESPONSE MEAN. '80.000 34.500 33.051 100.000 33.900 33.051 ,a*c'NO-LINEAR INTERPOLATION ESTIMATE CAN BE, CALCULATED FROM THE INPUT DATA. SINCE 'NONE. 0F THE (POSSIBLY POOLED) GROUP RESPONSE MEANS WERE LESS THAN 75.0% OF THE CONTROL RESPONSE MEAN. ac********************************************************** BOOTSTRAP PROCEDURE TO ESTIMATE VARIABILITY o-OF THE ESTIMATED ICP c*********************************************************** 'oad BOOTSTRAP' ESTIMATES OF ICp FOR ALL RESAMPLES WERE ABOVE THE .IGHEST CONCENTRATION /.% EFF. H j l sB00TSTRP)

. =. ~. SQN 0998 September 916.1998 INITIAL AND FINAL WATER CEMISTRY (FATHEAD) Initial Chemistry ~ Fish - Control 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.9 24.8 25.3 25.0 25.0 24.9 25.3_ 24.8-25.3 25.0 DO 8.4 8.4 8.4 8.4 8.4 8.3 8.3 8.3 8.4 8.4 pH 8.3 8.3 8.2 8.2 8.2 8.3 8.2 8.2 8.3 8.2 Cond 332 332 336 333 331 332 341 331 341 334 Hard 4.4 X20~ 88 88.0 88.0 88.0 Alk 6.9 X10 69 69.0 69.0 69.0 TRC Concentration 25% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.0 24.9 25.2 24.5 25.0 24.9 24.8 24.5 25.2 24.9 DO 8.4 8.2 8.3 8.3 8.3 8.0 8.0 8.0 8.4 8.2 pH 8.2 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.2 8.1 Cond 297 302 300 301 307 299 299 297 307 301 Concentration 39.2% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.0 24.9 25.4 24.5 24.9 24.9 24.9 24.5 25.4 24.9 DO 8.2 8.1 8.2 8.2 8.1 7.9 7.9 7.9 8.2 8.1 pH 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 Cond 275 276 276 277 277 277 277 275 277 276 Concentration 49% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.1 25.0 25.2 24.5 25.0 25.0 24.9 24.5 25.2 25.0 DO 8.1 8.0 8.1 8.1 8.0 7.8 7.8 7.8 8.1 8.0 pH 8.1 8.1 8.0 8.0 8.0 8.0 8.0 8.0 8.1 8.0 Cond 260 261 260 260 261 260 260 260 261 260 Concentration 80% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.9 24.9 25.2 24.5 25.0 25.0 24.8 24.5 25.2 24.9 DO 8.1 7.9 7.9 8.1 7.9 7.8 7.6 7.6 8.I 7.9 pH 8.0 8.0 7.9 7.9 7.9 7.9 7.9 7.9 8.0 7.9 Cond 212 210 210 210 210 209 210 209 212 210 Concentration 100% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.8 25.0 25.2 24.5 25.0 25.0 25.0 24.5 25.2 24.9 DO 7.7 7.9 7.5 8.1 7.7 7.4 7.4 7.4 8.1 7.7 pH 7.9 7.9 7.8 7.9 7.9 7.8 7.8 7.8 7.9 7.9 Cond 179 177 177 175 177 176 177 175 179 177 Hard 3.5 3.4 3.5 3.4 3.5 3.4 3.4 X20 70 68 70 68 70 68 68 68.0 70.0 68.9 Alk 6.2 ~ 6.3 6.3 6.3 6.2 6.4 6.2 X10 62 63 63 63 62 64 62 62.0 64.0 62.7 TRC <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 8mr.JLaD

= _ _ - _ _. _ Intake 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.0 24.9 25.2 24.5 25.0 24.9 25.0 24.5 25.2 24.9 DO' 7.3 7.1 7.2 7.9 ,7.0 6.6 6.6 6.6 7.9 7.1 pH 7.8 7.7 7.7 7.8 '7.7 7.6 7.6 7.6 7.8 7.7 Cond 178 177 177 175 176 176 177 175 178 177 Hard 3.5 3.4 3.3 3.5 3.5 3.4 3.4 X20 70 68 66 70 70 68 68 66.0 70.0 68.6 Alk 6.2 6.3 6.1 6.3 6.2 6.2 6.2 X10 62 63 61 63 62 62 62 61.0 63.0 62.1 TRC <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 Upstream 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.0 25.0 25.2 24.9 25.0 25.0 24.9 24.9 25.2 25.0 DO 7.5 7.5 7.2 7.7 7.2 7.6 7.4 7.2 7.7 7.4 pH 7.8 7.8 7.7 7.8 7.7 7.8 7.8 7.7 7.8 7.8 Cond 178 177 177 176 176 175 177 175 178 177 Hard 3.4 3.4 3.4 3.5 3.4 3.4 3.4 X20 68 68 68 70 68 68 68 68.0 70.0 68.3 Alk 6.2 6.1 6.2 6.3 6.1 6.1 6.2 X10 62 61 62 63 61 61 62 61.0 63.0 61.7 TRC <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 1 Final Chemistry j FISH Fish - Medium 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.0 24.9 25.0 25.1 25.1 25.3 25.2 24.9 25.3 25.1 DO 6.5 5.9 5.4 5.7 5.0 4.9 5.0 4.9 6.5 5.5 pH 7.8 7.7 7.7 7.6 7.5 7.5 7.5 7.5 7.8 7.6 Cond 344 346 346 348 353 354 350 344 354 349 Concentration 25% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.0 24.8 25.1 25.2 24.7 24.7 25.0 24.7 25.2 24.9 DO 6.4 5.9 5.2 5.3 4.8 5.0 4.4 4.4 6.4 5.3 pH 7.7 7.6 7.6 7.6 7.5 7.5 7.5 7.5 7.7 7.6 Cond 307 306 305 309 312 309 308 305 312 308 Concentration 39.2% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.1 25.0 25.2 25.1 25.2 25.4 24.9 24.9 25.4 25.1 DO 6.2 5.5 5.7 5.5 4.4 4.5 5.0 4.4 6.2 5.3 pH 7.8 7.6 7.6 7.5 7.5 7.5 7.5 7.5 7.8 7.6 Cond 283 282 283 284 287 289 285 282 289 285 Concentration 49% 1 2 3 4 5 6 7 MIN MAX MEAN . Temp 25.0 25.0 24.9 25.1 25.2 25.3 24.9 24.9 25.3 25.1 DO 6.1 5.7 5.0 5.2 4.8 4.9 5.0 4.8 6.1 5.2 pH 77 7.5 7.6 7.5 7.6 7.5 7.5 7.5 7.7 7.6 Cond' 266 266 266 266 269 268 267 266 269 267 hwa 2 d3

Concentration 80% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.0 24.9 25.1 25.1 25.2 25.1 25.1 24.9 25.2 25.1 DO 6.1 5.7 5.6 5.3 4.8 5.2 5.3 48 6.1 5.4 pH 7.8 7.6 7.6 7.6 7.5 7.6 7.5 7.5 7.8 7.6 Cond 220 217 217 218 218 217.217 217 220 218 Concentration 100% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.1 25.0 25,0 25.1 25.2 25.2 25.0 25.0 25.2 25.1 DO 6.2 6.0 5.4 5.4 4.5 4.9 5.5 4.5 6.2 5.4 pH 7.8 7.6 7.6 7.5 7.4 7.5 7.5 7.4 7.8 7.6 Cond .188 184 183 184 185 183 184 183 188 184 Intake 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.1 25.0 24.9 25.0 25.1 25.4 25.0 24.9 25.4 25.1-DO 6.9 5.7 4.9 5.2 4.3 4.6 6.1 4.3 6.9 5.4 pH 7.7 7.5 7.5 7.5 7.4 7.5 7.6 7.4 7.7 7.5 Cond 185 180 183 182 182 180 184 180 185 182 Upstream 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.9 24.9 25.1 25.0 24.9 25.0 24.7 24.7 25.1 24.9 DO 6.0 5.5 5.3 5.2 5.0 5.0 4.8 4.8 6.0 5.3 pH 7.8 7.5 7.5 7.4 7.5 7.5 7.4 7.4 7.8 7.5 Cond 185 180 181 181 181 180 181 180 185 181 Test Temperature Mean 25.0 Minimum 24.7 Maximum 25.4 Excel Reviewed by: Tns /s os. K-5 / Word Review by: Cut o s. e e -51 Page 3 of 3

_ - - _ _ ~ l SQN 0998 September 9-15.1998 INITIAL AND FINAL WATER CEMISTRY (CERIO) Initial Chemistry CERIO 1 Errol 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.0 24.8 25.3 25.2 25.1 25.3 24.8 25.3 25.1 DO 8.3 8.3 8.3 8.3 8.2 8.2 8.2 8.3 8.3 l pH 8.2 8.2 8.2 8.2 8.2 8.2 8.2 8.2 8.2 Cond 320 327 325 328 333 332 320 333 328 Hard 4.3 4.4 X20 86 88 86.0 88.0 87.0 ~ Alk 6.3 6.5 X10 63 65 63.0 65.0 64.0 TRC Concentration 25% 0 1 2 3 4 5 6 MIN ' MAX MEAN Temp 25.0 24.8 25.2 25.1 25.0 25.2 24.8 25.2 25.1 DO 8.3 8.2. 8.2 8.2 8.1 8.0 8.0 8.3 8.2 pH 8.2 8.1 8.1 8.1 8.1 8.1 8.1 8.2 8.1 Cond 286 287 289 288 291 294 286 294 289 Concentration 39.2% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.0 24.8 25.4 25.1 25.1 25.2 24.8 25.4 25.1 DO 8.2 8.1 8.0 8.1 8.0 7.9 7.9 8.2 8.1 pH 8.1 8.1 8.1 8.0 8.1 8.1 8.0 8.1 8.1 Cond 267 267 267 268 272 265 _265 272 268 Concentration 49% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.0 25.0 25.2 25.4 25.1 25.2 25.0 25.4 25.2 DO 8.1 8.0 8.0 8.1 8.0 7.8 7.8 8.1 8.0 pH 8.1 8.0 8.0 8.0 8.0 8.0 8.0 8.1 8.0 Cond 253 253 253 253 258 249 249 258 253 Concentration 80% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.9 24.9 25.2 25.4 25.1 25.1 24.9 25.4 25.1 DO 7.9 8.0 7.8 8.0 7.8 7.6 7.6 8.0 7.9 pH 8.0 8.0 7.9 7.9 7.9 7.9 7.9 8.0 7.9 Cond 207 207 199 197 207 207 197 207 204 l Page 1 of 3

1 Concentration 100% 0 1 2 3 4 5 6 MIN MAX MEAN i Temp 25.0 25.1-25.2 25.2., 25.2 25.4 25.0 25.4 25.2 ) DO-7.7 7.9 7.5 8.1 7.7 7.4 7.4 8.1 ~7.7 pH 7.9 7.9 7.8 7.9 7.9 7.8 7.8 7.9 7.9 Cond 179 177 177 175 177 176 175 179 177 Hard 3.5 3.4 3.5 3.4 3.5 3.4 X20 70 68 70 63 70 68 68.0 70.0 69.0 Alk 6.2 6.3 6.3 6.3 6.2 6.4 X10 62 63 63 63 62 64 62.0 64.0 62.8 TRC <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 Intake. 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.0 25.0 25.3 25.1 25.2 25.2 25.0 25.3 25.1 DO 7.3 7.1 7.2 7.9 7.0 6.6 6.6 7.9 7.2 pH 7.8 7.7 7.7 7.8 7.7 7.6 7.6 7.8 7.7 Cond 178 177 177 175 176 176 175 178 177 He:d 3.5 3.4 3.3 3.5 3.5 3.4 X20 70 68 66 '70 70 68 66.0 70.0 68.7 ' Alk 6.2 6.3 6.1 6.3 6.2 6.2 X10 62 63 61 63 62 62 61.0 63.0 62.2 TRC <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 Upstream 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.9 24.9 25.3 25.3 25.2 25.1 24.9 25.3 25.1 DO 7.5 7.5 7.2 7.7 7.2 7.6 7.2 7.7 7.5 pH 7.8 7.8 7.7 7.8 7.7 7.8 7.7 7.8 78 Cond 178 177 177 176 176 175 175 178 177 Hard 3.4 3.4 3.4 3.5 3.4 3.4 X20 68 68 68 70 68 68 68.0 70.0 68.3 Alk 6.2 6.I 6.2 6.3 6.I 6.1 X10 62 61 62 63 61 61 61.0 63.0 61.7 <0.1 <0.1 <0.1 <0.1 <0.1 TRC <0.1 <0.1 <0.1 Final Chemistry CERIO Cerio - Medium 1 2 3 4 5 6 7 MIN MAX MEAN DO 7.8 7.7 7.8 7.7 7.6 7.7 7.6 7.8 7.7 pH 8.1 8.1 8.2 8.2 8.2 8.2 8.1 8.2 8.2 Concentration 25% 1 2 3 4 5 6 7 MIN MAX MEAN DO 7.6 7.7 7.8 7.6 7.5 7.6 7.5 7.8 7.6 pH 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 hca 2 of 3

~ Concentration 39.2% 1 2 3 4 5 6 7 MIN MAX MEAN DO 7.7 7.7 7.8 7.6 7.5 7.7 7.5 7.8 ~ 7.7 pH 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 Concentration 49% 1 2 3 4 5 6 7 MIN MAX MEAN DO 7.7 7.7 7.8 7.6 7.5 7.7 7.5 7.8 7.7 pH 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 . Concentration 80% 1 2 3 4 5 6 7 MIN MAX MEAN DO 7.6 7.7 7.8 7.6 7.5 7.7 7.5 7.8 7.7 pH 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 Concentration 100% 1 2 3 4 5 6 7 MIN MAX MEAN DO 7.7 7.7 7.8 7.6 7.5 7.7 7.5 7.8 7.7 pH 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 Intake 1 2 3 4 5 6 7 MIN MAX MEAN DO 7.7 7.7 7.8 7.6 7.5 7.7 7.5 7.8 7.7 pH 8.1 8.1 8.2 8.1 8.1 8.1 8.1 8.2 8.1 Upstream 1 2 3 4 5 6 7 MIN MAX MEAN DO 7.7 7.7 7.8 7.6 7.5 7.7 7.5 7.8 7.7 pH 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 Cerio Final Temperatures Day 1 2 3 4 5 6 7 Rep # 1 24.1 24.6 24.4 24.8 24.9 24.8 2 24.2 24.7 24.5 24.8 25.2 24.8 3 24.2 24.7 24.4 24.7 25.1 24.9 MIN 24.1 4 24.I 25.1 24.6 24.6 25.0 25.0 MAX 25.5 5 24.6 24.9 25.1 24.9 25.1 24.8 6 24.8 24.8 25.3 25.2 25.5' 24.9 MEAN 24.8 7 24.4 24.8 24.9 25.0 25.4 24.9 8 24.1 24.6 25.2 25.0 25.2 24.8 9 24.5 24.8 25.2 25.1 25.3 24.8 10 24.2 25.0 24.9 24.8 25.0 24.9 Excel Reviewed by: Cut 05-t< o Word Review by: (- M -e<-91

INITIAL CHEMISTRY Page 1 of 2 Test ID:50N Chronic fnitiation Date: f ff Time 137o Test Organisms: P. oromelas Termination Date: de/.k,.f/ Time / :t 3 o Day l 0 l By l 1 l By l 2 l By l 3 l By l 4 l By l 5 l By l 6 l By l IM M m t Sample ID a Concutration: Control (F-medium) S Leg # 0/th n/0J 7tI5 ' O/W P37 4/06 BTT 0100 BD 0/v0 on !?/o* d2X l 12 ?/2M 22.s /2E:3 I ' !.fl.11 o 1 9'.1/ & c t Temp.- UW* 21.1 D+'t l'. 5/2 Y. 5 2'.4/ ~2C 1 8e Y

f. Y V. 3 l

(f. 3 l DO -VA' f.' V GP f.$4 $A. pH VA' f/ 3 T. 7 f.2._ P 2-E 7-

7. f l

f' 2 l i J t Cond. 33p J 332 lf 33G 33.3 J 33( b 3,z b-5yr Hard.(x20) V. +' (,kt Alk.(x10) /.,. 9 D33 TRC nk Sample ID; Concentrationi Outfall.101-7.5.0W 4M ~ Leg # N/A N/A N/A N/A N/A N/A N/A Temp. -UW* 2$~o rn) Z YS? TO 2 S~< 2. 70 24 3 bl5

2. 5. 0 OH z Y.1 yr f/ R V bT5

7. 3 t

g. D

$O l DO VA' W. y S. 2. S,3 t M3 pH -VA' W.)-, 3.I ?.I l Tl l Pl fit T,( l 300 [1 3o( V Jo 7

z. q q i

2 99 b Ccnd. c.qcy 9 v' S o z. s-Hard.(x20) Alk.(x10) TRC l Sample ID s Concentratian: Outfali 101-39.2W-

t j Log #

N/A N/A N/A N/A N/A N/A N/A i Temp. -UW* J S. b D7) ZY9 915 ZiY 125 BH.S 5% 249 b_p z y. 9 sr) 2Y? sif - DO UA' 'F. 2. l S./ R. 3_. I 6' 2-l

y. grf

7. 9 g

79 j C pH -I!A' [f, I l 8.( \\ Z.l 1 R. / l P./ 2.f l

7. (

z. v ;r 2 77 Cond.

,375 F pM b .176 t/ gpp 6' 777 Hard.(x20) Alk.(x10) TRC SampiaID-Concentration Outfall 101-49.0%J s Log # N/A N/A N/A N/A N/A N/A N/A Temp. UW* 25,I bT1 2 T. o m 25 2 RS b5 7 -i. o on 2 c.. an zY7 or)

7. 2

) 7I P. o ) 7.Q DO -UA' $.( f

f. o I

f.I I pH UA' ?I f./ \\ 8.0 \\ E. O

f. o l

g. c l

h Cond. ,2 & l) j f (l,/ rV l1.60 ?Ls t4l / f I 2 c. o .v E6 o 7 Hard.(x20) Alk.(x10) %,w TRC l Sample ID - Concentration: Outfall 101 '80.0 % - Log # N/A N/A N/A N/A N/A N/A N/A ._ Temp. -UW* 2 </,') bD ZY.i' rrs 25 7-ni D4.5 DT1 z<o on z r..) op z y er rz) DO-UA' ' f, j g p' 6an g 1 9, C) { Cf 7.7 g y.5 ( q. c, t pH -UA' Sf,(') l R. () 79 l ":7 g 7.f l 03' 3. 9 9 I

7. 4 Cond.

g/4 c/ Z/r) D ,q lo (J 'Z t o a 2-f o 251 c. 2ro d Hard.(x20) l Alk.(x10) l TRC Bh d[l kl] Y Nd AlI W N / o j Notes: i Initial /Following aerstion (inichem; effective 4-29 97) Approved By: M

InitiaVWarmed

. - - ~. -. - 1 INITIAL CHEhUSTRY Page 2 of 2 Test ID: SON Chronic ' Initiation Date: 9-f-fp Time _ / 33o Test Organisms: P. vromelas Termination Date; os-/04 f Time _/336 Day l 0 l By l 1 l By l 2 l By l 3 l By l 4 l By l 5 l By l 6 l By Sa rn pie ID r Concentration: Outfall 101-r 100.0%.1 Log # 3119 Ms Jt if DJ1 3f99 DP 'bl 9 / D15 3t19 bo Jzot yr) Jzs4 pr> Temp-IAV* 1.1/J(/.2 I l.f/2S O l /. //M. 2 I !. ]l2 4 T / /lLf

t.{/ 21 *

/.1/ t s.o r DO -VA' '7. 7 / 7.9 / ' 1. :r' f

7. /

7.7

j. '/

l 'p. $/ pH.UA' 9,4 j 39 /

7. T l

9.9 \\ '). 9 q.9 t, 7) Cond. / 7(f } /p7 l. 177 / y f-U / q ~} /94 /77 Hardfx20)

3. 5 (p.

J. y Inr 35 -J2<* 3.4 G~ 3. 5-3.4. 2:c 3. c./ AlkJx10) h.2_ M5 (,. J h (o,3 JdL (,. 3 C~ (,. f - V 6,4 Icf

4. 2-p TRC 4 D, \\

p zo.t DJs O.L DTJ t o.t nr1 go.t 29 <. O I en Sample ID 2 Concentration:. Intake 100.0%5 s Log # 3171 rrfs '3I95' M 3iT f-3c 7 i / 2-DD 3/19 3 73 n.o 2, 375 3 2. e o d' Temp. -IAV* m /,25,Q \\ 01/ZVf 0.?h5. 2-1 0& /21.S \\ 1f/ t.S* O bc/ 2 t'.9 S3/ 2 To \\ DO -UA' M3 / ~4. ( ' 7. 7 I '7. 9 'a n (,. (e 6 (o - pH VA' 9, Q l T.?

7. 7 l

P. T 1 77-

7. L l

'7. C. Cond. / "fp J /'47-k 177 / 75' Y / 7 /, /9G i/ /74 j Hard.(x20) 3.5 (,Att

3. </

DJ3 3.3 Scc 3.5 G~

5. r

{ 3,4 yef_ lit i Alk.(x10'r (,. 2. >3 ) (. 3 Li f.t 36*- (,. 3 V 42-V (s, 2. S6:'- ( 2. J TRC .< 0. I 1gS t.o.t VD LO.L 01) "la 4 ts. \\ or? ut s) O,e DT) Simple'ID ; Concentrationi Upstream; 100W f Log # '3I80 'P13 3 i l> PS) '984 5 off 9/97 W) 3 Zoo pr$ 32oy p15 3 2.oe OT' Temp. -IAV* o.1/o?S b i /o/25.o 1 0.'f/.25.'l f.5~/ 2M A D.W6 o e t/ fff.cdi o.?/2 V' ? l DO UA' W. S~ l ~~9. 7 1 h, 2.- ^7 ? i 7.

1. (,

j ' ?. y' 1 pH -UA' 7, 9

7. T

7. 7

7. 8 1

7. +

79 l % S' Cond. / ')' f /79 J (T] tt / '+ L. U f M, /q r J

y q.

Hard.(x20)

3. '/

(,rft

1. y m3 3.4-.

h 3.5

3. '/

3.4-Gr_. 3.y Alk.(x10) 6_ 2, m // ,(/ (.,,2_

fcc_

(,. 3 u (, / } (o. l

ftp,

(, 2.- E TRC (s.t 3,7 f <a./ 07) CD. L uTe "/ p 4e4 W) o.' ars 4o.< rp Sample ID L Concentration:) ai W+ Log # Temp. -IAV* DO -UA' pH -UA' Cond. Hard.(x20) Alk.(x10) TRC SampleID - Concentration:~ Log # Temp. -UW* DO-UA' pH -UA' Cond. Hard.(x20) Alk.(x10) TRC Yb D ih Ob h il t)I B y y { W ll U 'l Notes: initial /Following aeration. (inichem; effective 4-29-97) Approved By: tW

InitialAVarmed t

FATHEAD MINNOW FINAL CHEMISTRY Page 1 of 2 Test ID: SON Chronic Initiation Date: 9-f- f f Time / 3 To Test Organisms: P. vrome/as Termination Date:o4 A fr Time / A 16 il Day l 1 l By l 2 l By l 3 l By l 4 l By l 5 i Bv l '6 l By l 7 l By l l1 Sample ID 2 Concentration: Control (F mediumF 4 Log # l Ofon m D loo (rkk,O/44 G 0100 a9 010o ta5 c100 nf> 0160' 414 Temp. l.25.0 (a 24.9 I 45.d t .2 s. i 2 5.I 25.3 2 5.1> l DO l 6.5

5. 9 59 I

5'. 7 1 5.0 4.9 5.o / pH l 7.7

7. 7

7. 7 I

7.L l 7.6 7.5 75 l Cond. l 344 ~Q/ 346 \\ / 3</G 34i 1/ 353 354 3So W

SampleID JConcentrationfiOutfall!10le25.0% k

~ 4 gma Log # l N/A N/A N/A N/A N/A 1 N/A N/A Temp. l 25.0 CVJ 24.9 CI'.]l 25./ ca .2 5.1- ,> il 24.9 grf> 2Lq g 1s.o Crk! 5.o 4.L l 1 L 2. 5.1 A. 9 DO l 6.4 5.9 pH l

7. 7

'7. 6 7, (> 7.0 1

7. 5

7. 5 7.5

,l Cond. l 3o7 y/ 306 \\ / ,50 S 309 t/ 3/1 3o9 / 7o g (/ I Sampl6ID T Concentration: Outfall:101-392%F Log # N/A N/A N/A N/A N/A N/A N/A i Temp. 25.I (. l(ll. 25.o (<kX J f.1 Cu. 25'.i STS 2 5 2. 6 75 25.4 ys ]4.9 G4# 4.4 1 4.5 \\ 5.o DO (o. 2 5.5 5, '7 i $.s ~ i l pH

7. 7 7.6 24 l

7.7 l

7. 5 l

7. 5 I

7. 5

\\/ Jf3 k 2N (/ 2F7 , i, 2 g9 / 2 95 y l Cond. 2F3 \\ / 292 I SampleID - Concentraliant Outfallt101-49.0%i! w Log # l N/A N/A N/A N/A N/A N/A N/A Temp. l 25.0 G1 25.o (ekt J t4 9 Cen .25.1 to 25.1 21( 25.7 /n5 .24.9 ((+ DO l 4.f 5.7 5.0 ( 5. t. \\ 4.8 4.9 1 _q.o I ~ pH l 7.7 1

7. 5

'14 / 7.5 f 7.6 7.5 7.5 L 2(.t V 269 2G8 9 267 y Cond. l 24(, 'V .24 C \\./ AG& l r 1 Sample ID ; Concentration: Outfalli101e80.0%7 Log # l N/A } N/A N/A N/A N/A N/A N/A I Temp. I 2 5.o DIt 24.9 CV)L

25. /

cu .2 s. ) m 2 s.1 p)S 2s.1 17r3 2 s.; /W: 7 DO l 6I 1 f.7

5. (o l

O i 4.g i g1 { g.3 ~ ' j pH l

7. W

7. 6

7. 4 U

l 7.5 I 7.6 \\

7. 5 I

Cond. l '22 o v 2l7 \\/ A/? e lif V 2/F J 217 4 217 i/ ! Sample ID - Concentration::Outfalt 101-100.0% i M wi8 F I Log # _? ; '7 f frP// 31g4 M S t f7 ') i f I + ~3: 1 y 'm 32oi pT5 3249 &f' .i Temp. 2.5.i f' 2.5.0 ,.7 5. () Gp_ .25.t

ti9, 25.1 1

.25.1 25.o l ij DO G.2 4.0

5. F l

s.4 4.5 I 4.9 f.5 / i pH

7. 7

7. 6 26 7.5 I

74 7.5 I

7. 5

( if# t/ /85 153 6 I?4 VI V W Cond. /87 v 174. \\/ /f 3 ~ ^ -l .I Sample ID - Concentration: Intake-100.0% Log # 31 v7 ('b/ 3185 CM 3t i t-W 3if2 m 7<ff m Stot 215 3210 (J? ' 35.4 25.0 l ll Tcmp.

15. l l

3 g,o Qd. 9 w 2s.o [b8 25.1 li DO

g. 9 l

5. /

+'q i s. 2-i 4.3 4.6 6./ / H pH

7. 7 75 f

'Z5 I 73 1 7A.

7. 5 l

7. 6 I

n Cond. /g5 \\/ lFo W /98 1R 1/ l71. /90 ! T d-W M/ ML d "*"*% c2L Gdt V / Notes: i

FATHEAD MINNOW FINAL CHEMISTRY Page 2 of 2 Test ID: SON Chronic Initiation Date: f ff Time /330 Test Organisms: P. cromelas Termination Date bf-//,.49 Time /31b Day l 1 { By l 2 l By l 3 l By l 4 l By l 5 l By l 6 l Bv l 7 l Bv Sample ID ; Concentration: Upstream '- Log # 3lTo (,kl( 31 % CrkL 34 1 % W 3/92 on 3 2c 0 on J 2 0.3 Yi1 32fl QL 7 Temp. 24.9 ) 24.9

5, /

c-M.o op M.9 25.0 24.7 1 DO 6.0 l 5.5 i S. 3 \\

5. L l

5.0 5.0 -1. S' pH 7, y rl.g M M / 7,5

7. 5 l

7,4 Cond. 185 \\/ 180 \\/ ff/ lll k' 181 b 110 !$l V ~ .: Sample ID ; Concentration!! d Log # Temp. H DO pH Cond. I Sample ID 2 Concentration: Log # Temp. DO pH Cond. SampleID - Concentration: Log # Temp. DO pH l Cand. k Sample ID - Concentration: 1 Log # l l l Temp. I DO I pH I Cond. ll SampleID ' Concentration:: ll Log # 1 Temp. ll DO ll pH l Cond.

! SampleID Concentration:

1 Log # Temp. ll DO ll pH ll Cond. MV Cut Gn-Cd$ _. Notes:

1 INITIAL CHEMISTRY Page 1 of 2 Test ID: SON Chronic Initiation Date: 9-v'-ff Time 1720-/37 5' Test Organisms: C. dubia Termination Date: 69_/G.ff Time n.14 Day l 0 l By l 1 l By l 2 l By l 3 l By l 4 l By l 5 l By l 6 l By SampleID J Concentrati~on:2 Control (C-medium w/oEDTA)(1)s Log # 27(. z. Of> .on2 tD 2742-ry/ 27p w

2. 7 (, r v.n z y cs-7tr<,

Temp.- 1/W* ,12.6/)S.b 1 E 9'8 Y

21. ?/ 35*J Lt. L!! 5* 1

'n.o/t5.I z r.r/% s.') \\ 8.3 7.f . KD $3 W 2> l T. L.

f. z-l DO -VAf pH VA 7.1

$2 / P. 2

2.

l T. 2._ i

f. '2 i

T Cend. 3,*L2) $/ 72.7 J/ .725-V 32F // pyy J. 332-ik 13 2'/c

t. 9' 25')

Hard.(x20) 4 Alk.(x10) (y ',h pi9 6. 5" Le nC $n iSample ID 2 Concentration:N Outfall 101-25.0% (2) % Log # N/A N/A N/A N/A N/A N/A N/A Temp. -UW* n't 5, b ,w) 2<Y W W ' 7 5~. 2. An

13. t DD LSo cm 25 2

.p3)

8. Z-P./

go { DO -UA' F. ~3 F. L. 12 0 72 i pH -VA' f.2. / 7.( l F. a

f. /

l P.r

f. /

l Cond. ,7ffs b

2. 7 ?

sv 2 89' 288 V 29l J 9.-rnct V Hard.(x20) W Alk.(x10) c.f,f e RC J SanipM ID.' Conmh ationf Outfill 101 39.2% (3)T Log # N/A N/A _ N/A N/A N/A N/A N/A Temp. -UW* o} S,6 353 L f'. I' W ' 25.Y ph 2 S.I pp ES. t 7v L 1. 2 M 1 79 l R. / \\ F. D DO VA' T2 }

8. (

_ DD F. n r f.I

f. t

f. o l

2. t l

& ?. d/ I pH -VA' 9.(

2. c,.;r J

26F M Z72 J

24. T Cond.

c,7M J 2 ( 72 Hard.(x20) Alk.(x10) l TRC Sample ID - Concentrationn Octfill 101249.0% (4)N Log # N/A N/A N/A N/A N/A I N/A N/A m 2f0 M-25.2, try 15V 3D 2L g p r. p n75 Temp. -UW* .25, d I DO -UA' 9.( ) F. O PTS

7. O

~ R/

o

7. P' pH -VA' ff, l l

?.0 l W. O f.O I 5" O S. D i Cond. M_s 2.53 L 7 5~3 v 253 Lt 2.77 2yg } Hard.(x20) Alk.(x10) TRC l . Sample ID - Concentrations Outfall 101-80.0 % (5)1 ~ Log # N/A N/A N/A N/A N/A N/A N/A Temp. -UW* c?V, $ g 79'9 Q CL 42, pf$

e. f. y pT4 2 r.t gjy rf /

7p DO-VA' ~). q 'l

g. O gS 7.g 7()

l

7. ?

l ~).(, l pH -VA' y,j) l To { q_9 '). 9 l ?9 \\ T[,4 l Cond. Af)*)

z o ~?

b / f'f t/ / 'l 7 f/ z.o y V . c ~+- V V Hard.(x20) Alk.(x10) TRC 9h &f) Y 6W N y / V' Notes: nm t sanrwMhMeromr,tmaxa (inichem; effective 4-29-97) Approved By: M

INITIAL CHEMISTRY Page 2 of 2 Test ID: SON Chronic rnitiation Date: 04-o4-6! Time /DJ -/M Test Organisms: C. dubla Terrmnation Date: 8 9-15-f 8' Time 13e Day l 0 l By l 1 l By l 2 l By l 3 l By l 4 l By l 5 l By l 6 l By 1 Sample ID r Concentration: Outfall: 101# 100.0% (6) ? mW s Log # it ? S

Cr>

3f 89' pf) 3t f/ OT) 394t ul M 41 kn stot sp Temp.- UW* o75.0 L

z. r.1 nk ' 26. *l.

L t r. t-L

15. t L

2 C. Y I DO -UAf I pH -VA / Cond. / / < / r* Hard.(x20) 7 1 (_,( H 4 l) 'f t' i Alk.(x10) [ t' / s TRC

Sample ID ; Concentration 5 Intake (7Ys ms 3tfr 51 /3112 OD

>t 92 rp 5(99 vr? .gz o z w i; Log # 31 **? f ms Temp. -UW* 2 50 l LS.s W 25.3 V L 5. t 1. 252 1 25 2. .b t l DO -UAT I pH -UA / Cend. (r. k 3 I , // 1 Hard.(x20) /' (,)50 % pi L. n - Alk.(x10) \\ / TRC Sample IDT ConcentratiandUpstream (5) > ' W6aAJ !! Log # 3/8D M5 31 F(, N. 3rf 7 _aD 5tfk3 m wo at 3'263 n9 Temp. -UW* e29'q ]. 24'.9 W 23.1 2%3 1 t 5. t L 7 s.c DO -UAI I pH -UA t ,/ Cond. f f f' ,r M ,\\ LG ') l f Hard.(x20) 5 (' V () 4 (\\ L L / Alk.(x10) TRC Sample ID - Concentrations i-in Log # Temp. -UW* DO -VAT T pH -VA Coed. Hard.(x20) Alk.(x10) TRC l SampleID-' Concentration 5 +t

N Log #

Temp. -UW* f DO-UA pH -UA' Cond. Hard.(x20) Alk (x10) TRC g W Y kY ( j j Notes:

AwWnV/Wasaed r Initial /Followins aeration -

Agroved By: fg_

___m..___- DAPHNID FINAL CHEMISTRY Page 1 of 2 Test ID: SON Chronic Initiation Date: 7-f-fP Time /Ju-t nr-Test Organisms: C. dubia Termination Date: es. PHf Time i3ro Day l 1 l By l 2 l By l 3 l By l 4 l By l 5 l By l 6 l By l 7 l By ' N Wii

s SanipleID ; Concentrationse Control (C-medium w/o EDTA) (1Fli Log #

l 2 76 2 Os t _ J 7t.,p pr) 'L/6 2 W 2.~.N. Z. sti)

2 1 (, s' W- ' ylt,s W

DO l 3-( O tk 79 I 4.R 1 ^7 7 DN 7 (o l 77 l pH l 1 - (. V' f.4 d' ?. L V V%- .b $ L-Y YL-V JSamplilD - Concentrations OutfallJ101:25.0%f(2W W, l ~ Log # N/A N/A N/A N/A N/A N/A N/A DO 7(o 06 77 D>S 77

ff 7/o A*

7f 9 H~ 2(,o D t*' i pH 8l V Pst b 8'- ( b $I V f( l V 8.( V Sample ID IConcentrations OutfaIL1101:39:2% (3)W m N/A N/A Log # N/A N/A N/A N/A , N/A DO 37 D/4

q.,

arr

7. a-tro 2c DP e v-3%s qu

7. 7 op pH T(

T.t L %d 1, 8'- ( h $. I V <t. I k ' Sam'ple ID4 Concentration $Outfall.101F49.0%T4)S < ^ Log # N/A N/A N/A I N/A N/A ] N/A N/A DO )7 .0fi

7. p 2/S 97 DD

7. (.,

.D? 73 QH 77 6" pH %i 8.c f 1.I l' SI V &I Y B-(

SampleID Concentration:tOutfall:101f 80.0%(5)?

Log # N/A N/A N/A N/A N/A N/A N/A DO 7 (, Sfi ?.7 a7/ IrJ1

7. (.,

Mf

7. $~

()if 77 %v 7.T pH l W. ( 4-f.e b C.t V 4'I Yl 3I V 9.( k* Sampla ID - Concentrationn Outfall 101r100.0% (6) ? ^ s Log # 3l7P dif ') # fV 657 7/ 9/ off 3/1/ en 7 ( S.V V 3;:o/ bi4-DO 77 VS 7.7 3

7. T

/

7. (-

Q4 7 5-b 77 1 pH f!I. \\ 4 f.t T.t b $-l N' $. I 4' C,(- I Y I Sample IDIConcentrationiintake (7)> W m W Log # 3,7-1 M 3ir5 off 3, f 2. g5 )( f 2, m 3(T9 Stb 32o2 D4 DO 77 D,ll T 7 /

z r l

7G 0% 7 5- } 7, / [ pH

f. \\

4-6,t b %. L V S-l T-(

q. I l Sample ID - Concentrationt Uostream"(8) ?

Log # % fo nys 3 /fl,, D11 3IG 3 p15 'I t 'i 3 an R.;too D tt" %o3 OV DO ~'77 Dti

7. 9

( '1% I 7(o h& ~7.T t ?? \\, pH f(. I V 5.( k f.t b (( I Y T-I A T*( f b NM C B t Notes: Approved By: N (finlched; effective 5-5 97) e

DAPHNID FINAL TEMPERATURES Test ID: SON Chronic Initiation Date: 9-f-ff' Time U2o-cys-Test Organisms: C. dubia Termination Date: 9-Is-4V Time /A20 Day 1 2 3 4 5 6 7 By DMW fM3 '8 p3) #2 W *r W W Rep.# 1 cW l kV.6 t-V. 9' e29. G f2& 4 M.6~ 2 M-u 24'.7 2 Y. C' c2N.{ d2 S,2 - 348' 3 M. U 7y ? sY.9 ,2.47 25 I 941 4

93. (

2S. t 2 9. (- 5L44 30 as.o 5 e71. (, 'Z 4'. 9 2f./ d47 ar. I M g' 6

14. cl 1 Y. 2 1 S. 5 e15. 2 -

Els.f c2.+9 7

24. Y 2 V. E i +' 9 ps.o 23 4 G47 8

M. ( z tr. G 2 f. f 25., A1. z_. ,a_v.cr 9 ,44. f t V. 9 t S. T - 2sI B5 3 M-S' 10

24 h 2 f. o 2g9 6Lt.Y 2T. o M9 Reviewed By: Dtf- -

4/f

h SD DW M i

(/ Approved By:

l Sequoyah Nuclear Plant Biomonitoring September 9-16,1998 i Appendix D Reference Toxicant Tests and Control Chart Information i

..._,_,-__os. . ~. _ ~ ~ ~ - -.. . MACLFIC2.XLs IC25 NACL Toxicity Test l Control Chart (Fathead minnow) Most Recent Testi i Test # Source Dats IC25 Mean S 2S Mean-2 Mean+2S CV% Control Mean WL (mgL) 17 TIL 04/23/97 1894 0.58 18 TIL 04/23/97 1838 1866 40 79 1787 1945 2.1 0.69 New Brine Shrimp Lot # 4434 19 TTL-- 04/30/97 1928 1887 45 91 1796 1978 2.4 0.46 20 TIL 05/27/97 2299 1990 209 419 1571 2409 10.5 0.36 - 21 TIL 07/01/97 2212 2034 207 414 1620 2448 10.2 0.62 22 TIL 07/29/97 l VOID. 70% Contro!.swwval l 23 TTL 08/20/97 1609 1963 254 507 1456 2471 12.9 0.56 24 TIL 09/02/97 2376 2022 279 558 1464 2581 13.8 0.53 25 TIL 10/24/97 2000 2020 259 517 1502 2537 12.8 0.49 26 TIL 10/31/97 2014 2019 242 484 1535 2503 12.0 0.60 27 TIL 11/29/97 1652 1982 256 512 1470-2494 12.9 0.65 28 TTL 01/07/98 2315 2012 263 525 1487 2538 13.1 0.54 i 29 TTL 02/04/98 2158 2023 254 508 1517 2333 12.5 0.49 I 30 TIL 03/12/98 2424 2055 267 534 1521 2590 13.0 0.46 1 31 TTL 04/01/98 2063 2056 257 513 1542 2569 12.5 0.46 1 32 TTL 04/01/98 2012 2053 248 495 1558 2548 12.1 0.56 New Brine Shrimp Lot # 4665 33 TTL 05/06/98 1857 2041 244 488 1552 2529 12.0 0.63 34 TIL 05/26/98 2020 2039 237 473 1566 2512 11.6 0.56 35 TIL 07/25/98 1985 2036 230 460 1577 2496 11.3 0.65 36 TTL 07/30/98 2052 2037 223 447 1591 2484 11.0 0.65 17 TIL 08/26/98 1837 2027 222 444 1583 2471 10.9 0.66 Old Brine Shrimp Lot # 4665 l I 7-DAY NACL REFERENCE TOXICA.VT DATA hmephalu promelas so88 - , Mi?;,,.[ pf

i:'!bi ' f P.@.'$(.<. :.l$fD ',j,7[ ~.[w, h,.,..,,1 f5
}...}j c.r-m-eve-wmmmmmmemmvemarn-mm. eme

.. /...... t"4m,E e c2J aw-a.- % <..s 3.:n. 3. - x s:ga - a w.M :' T 7 : = - -..,, :. - : = 7~; -.-u cu uos. jf 9!E.- ( '::3 i Z?M -.- umm.13D ?% ;;;.n ng:Q@gf 5j;j;gg;. .. J..i,.W - e~ _G.k.::.f a.- -.. f. -e-wan. tso P , l M' f:. saa s p i.: s :4.(gx.

S>:..;

e f d o,c.

n. /..,..

.^ ^ ' t w %i!!%F Ji;P m.St $ ik ' diOF ' im./ Wry.,.J A X: ......:...... 3 2.. i.L.x..:.L...dmw ng.h L. g '..;gg ,a;ig ...Cp%. :n% :.. .=-.:............ .m.... anoo.. .(Nh{f j $Ni{.*} y [~ lj{[ {. [ ';.[ ' ' see I IE S II.I 1 8 E

t S S S S I E

S 3 "3 3 I K. I E. 8 3 S. 5 S E. .E 8 I.S. E. E " e

8 v

1u.ean.mr= e tage !

.~ CHRONIC REFERNCE TOXICANT RECORD SHEET otel OC Study: Reflox # 37 37 OLp h..) 2. SH A..-r Test Organism: P. vromelas J J. Initiation Date/ Time: OF-u-fr/<orr to cu 'l 4. s i. Jh. v - Termination Date/ Time:0f oz. fr/o3r d.m e,t.< u m Control / Dilution Water: F-medium 00 98 Personnel: Ao 4<< Toxicant: Nacl Source: Fisher Scientific ib@f Lot #: Test Concentrations: Control. 0.8.1.2.1.7. 2.5. 3.6 c/L L.promelas: Soawn Date Tile # Hatch Date/ Time to Date/ Time 04-27-ffAa r os ts 91/cryr-Oarct/ dd<7 o 7t" [ [ A Y)))$ 8' A3 y ' d ' 5 ' 97 ICu: d>: ii n %

IG'1 Notes:

klY N ~ l 9 l' I 4% lP05'95' W HYY,.$'EC'9 g r

og &t,..Y:M FATHEAD MINNOW LARVAL SUR GROWTII TEST Page 1 of 1 s,. n est ID; RefTox # M Pans: Date/Tima of drying f - *4rff.ss-Fish: Date/ Time of drying 64-oMP//v/b ##' ,itiation Date:or.u ri Time-tosr-Pans: Date/ rime of weighing 1 z#/ zS Fish: Date/rimi of weighing o,-<ns//fo3a ermmation Date: 1 M4 7 Time - /o us- ' /A30 Sample ID/ Rep.

Alive Tare Tare + Fish Concentration Weight Weight MNotes M *'

Day 1 l 2 i 3 4 5 6 l 7 g g i Control Log # n0TY ..O d 4 ft CO M oo 9 'l 0 0 'M co i ? Do 'i "I l (F-medium) 1 IO /o /o to I /0 /0 n9F927 0.995fd l 2 IO to to lo /0 lb

0. 9 5'? 3,1?

D,994% '< ~ m. 3 to /0 to 9 1 9 9 0.973+8 \\0,993/d %y, 4 po ig to Io /u to i.:, o,9g g3 g,qqa 7y ' ' wEl By 'Tdd (rkt .D64 -- > W Jdc-o rt-03-5 Cr/J I Log # N/A N/A N/A N/A N/A N/A N/A l 0.8 g/L 1 /0 lo /0 /0 10 10 /C O. 7El L9 4,990 lolo I 2 (O /6 to to to /0 10 d.9'4 8 L 3 6,99 9 9 % 4-l~c M W 3 to /b to 10 to 10 M *"'~ 9 D. 980 t o d.98 7 4t1 (lL;dQiu 4 lo le to lo to /0 10

o. 9g3o e

() qq /qA w By Tec GE/G Dr4-O,, ot+--- rd d D W~ DD CNA I Log # N/A N/A N/A N/A N/A N/A N/A l 1.2 g/L 1 /0 9 9 cv 7 9 5

0. 9 F r. 5 5 6,94/69 f f,

<T 4 7 9 9 0.4tI(4, 4.9f949 I' 1.J 2 lo 3 10 to to lo to 10 /D

c. Tg'/. La n, 99 5M, l 4

(0 lb /O to to /0 to 0.9225'( A 99 '714 l By 3dd. (f-///f DW Cw DF 3dC. B ++-- D0'3 (d /C l Log # N/A _ N/A N/A N/A N/A N/A N/A I! 1.7 g/L 1 to @W /d o a ti 5' (

o. 't PC z.?

o.49/5 7 I 2 /0 to /C to /0 9 9

d. 97615-o, GMAO I l_hitilO

( 3 (0 /6 9f 4 "I 9 'l 4 o.92S'L,n

n. 99-1 fil \\

4 to lo to 6 7 9 9 0 9ys 9t n.99 / 75 l' OT By .sce._ L-kif D11 Cw OV 3s1. 0+- n:o CrKK l Log # N/A N/A N/A N/A N/A N/A N/A l l 2.5 c/L ' 1 /0 /C /# 9 9 9 9 o 'T7W5~ b().9f9ff I 2 10 10 to to 9 7 (o

o. 98t/o3 6GF(sys

( 3 (0 //> to to 4 8 7 D. 91 Y7(o n 9 f/7 74 4 (0 /(j /u IO 9 9 G o_9 70q g g GQ y31 ^ By i 3dd G 2/f DH ~~~ c# DA Jdd h eP-A:7) l [rU Log # dn "l a "l w Nla "l a "l w ^ln 3.6 e/L 1 /0 In /* (o '(-> fo C D 91'ff. le (),490 f(0 2 /0 /b to 9 h* 7 (o o998+3 d,9 99 28 3 to 10 9 7 6 3

0. 991/2.

ll0. 9415'/ 4 /0 //) 9 '7 7 5 0.98 @ S" O.92976 M' Bv scc (#1%4-D tt-c. 04-7dd_ 0 t+ - tas Crkk Log # M"" l l l 1 1 l I 2 ^ 3 l l I 4 By N o 1 2 3 4 5 6 7 LestcuukOS p-spille).t 4k km $ Fed-TimelBy: AM OWlsc) (esulot4-- c4nhs aw(?% g(uslw- %;,jj'1 Y"&YE E /an/ls' InVsa, mww Itisla nn kg. unkee-m w.- /dk/2s Igo/23 - Nosb-i+ovt49.-i~.o19,r r.cn /xt g,., rnio /ogr-RenewalTime: X Ib A 5 tb:3 b tott io g t o.t o Diluti:n Water: ooff 60W pr>9 9 cou now o o 'l 1, 0099 j R: viewed By: tA/l Ofh d[ ra r_ W .OtT"~~ W r-m e~~

Ref ton $37* 085 54615 FATIEAD DRY WEIGHT RECORD SHEET Penannel: DJS GKK Study /Date: ReRo.t #37 08/26/98. Old Brine Shrimp #4665 Pans:Date/Tirne of Drying:0941-98/0945 Fish:Date/ Time of Drying: 0942-98/1410 09-02-98/1035 094 3-98/0925 Pans.Date/ Time of Weighing:09-02 98/1225 Fish:Date/ Time of Weighing:09-03-98/1030-1230 dample ID Tarc WL Iarc + han WL han W L durvivmg a hali Survivmg Mean WL IC25 WL IC25 Mean WL survivmg Control g g g mg mg mg Mean Wt. (mg) 1. 0.98927 0.99580 0.00653 10 0.653 0.653 0.e 53 Control 2. 0.98838 0.994 % 0.00658 10 0.658 0.658 0.658 0.6o4 3. 0.98778 0.99368 0.00590 9 0.656 0.590 u.648 0.656 4. 0.98583 0.99274 0.00691 10 0.691 0.691 0.691 1. 0.98169 0.99006 0.00897 10 0.897 0.897 0.8 g/L 2. 0.97863 0.98734 0.00871 to 0.871 0.871 3. 0.98010 0.98747 0.00737 9 0.819 0.819 o.s 70 4. 0.98300 0.99192 0.00892 10 0.892 0.892 1. 0.98255 0.99109 0.00854 9 0.949 0.854 1.2 yL 2. 0.98166 0.98929 0.00763 9 0.848 0.763 3. 0.98620 0.99506 0.00886 10 0.886 0.b86 u.840 4. 0.98854 0.99712 0.00858 10 0858 0.858 1. 0.98629 0.99157 0.00528 8 0.660 0.528 1.7 g/L 2. 0.98685 0.99320 0.006T5 9 0.706 0.635 3. 0.98560 0.99267 0.00707 9 0.786 0.786 o.o3a 4. 0.98571 0.99175 0.00604 9 0.671 0.604 1. 0.98485 0.98988 0.00503 9 0.559 v o.503 k #I h Q 2.5 pt 2. 0.98403 0.98645 0.00242 6 0.403 0.242 MN 3. 0.98486 0.98774 0.00288 8 0.360 / 0.288 0.354 V 4. 0.99048 0.99431 0.00383 9 0.426 0.383 1. 0.98966 0.99080 0.00114 5 0.22% v 0.!!4 y Mt [M ~, 3.6 yL 2. 0.98873 0.98988 0.00!!5 6 0.192 0.115 g 3. 0.99112 0.99154 0.00042 3 0.140 / 0.042 0.uvo M 4. 0.98885 0.98976 0.00091 5 0.182 0.091 Reviewed By: Russell b os. oc - 9 l 1 Page 1 of 1

THE NUMBER OF RESAMPLES IS 80 &tt R e.% t 96 "37 To M 01 - d ' ' ' E Gldba W 23 -s 4(. t. 5

- - LISTING OF GROUP CONCENTRATIONS ( "; EFF.) AND RESPONSE MEANS ***

CONC. ( PeEFF ) RESPONSE MEAN MEAN AFTER POOLING .000 .648 .786 .800 .870 .786 1.200 .840 .786 1.700 .638 .638 2.500 .354 .354 3.600 .090 .090 THE LINEAR INTERPOLATION ESTIMATE OF THE TOTAL IMPACT CONCENTRATION FROM THE INPUT SAMPLE IS 1.8372. 3.600 .090 .090 THE LIN AR INTERPOLATION ESTIMATE OF THE TOTAL IMPACT CONCENTRATION FROM THE INPUT SAMPLE IS 1.8372. c22******************************************************.<** BOOTSTRAP PROCEDURE TO ESTIMATE VARIABILITY OF THE ESTIMATED ICp cx********************************************************** THE MEAN OF THE BOOTSTRAP ESTIMATES IS 1.8282. THE STANDARD DEVIATION OF THE BOOTSTRAP ESTIMATES IS .1127. AN EMPIRICAL 9 5. O?; CONFIDENCE INTERVAL FOR THE BOOTSTRAP ESTIMATE IS ( 1.6527, 2.0634 ). EOOTSTRP)

~. Cerio Chronic Control Chace IC25 NACL Toxicity Test Control Chart (Cerio) (KY range for cerio chronic 848 1216 mai ) TcstA Source Date IC25 Mean S 2S Mean 2s Mean+25 CV% 09 11L u3/3 li96 GT1 1233 AQS ooo 70 TTL 04/28/98 CTI 1026 1140 161 321 818 1461 14.1 AQS rood 71 TTL 04/28/98 CTl 1139 !!39 114 227 912 1366 10.0 Ec7 rood 72 ITL 05/1444 VOID 73 TTL 05/19/98 CIl 815 1058 187 374 685 1432 17.6 AQS Tood 74 1TL 05/19/98 CTI 920 1031 173 346 684 1377 ' 16.8 AQS Food 75 I~It 05/2668 CTI 1122 1046 159 319 727 1364 15.2 AQS Food 76 TTL 05/27/98 C11 837 1016 165 331 685 1347 16.3 AQS rood 77 TTL 06/02/98 CII 761 984 178 356 629 1340 18.1 ACS Food 78 TTL 06/02/98 CTI 884 973 170 339 634 1312 17.4 AQS Food 79 1TL 06/09/98 CII 663 942 188 375 567 1317 19.9 AQS Food 8u 1IL v6,uw98 GTI 393 lest was acd u.i mL os algas uutcaa or u.2 mL. not in control cnart at ilL uos iveve CTI 23 2 677 lis 557 32L 1434 JL7 AQs fooo 42 liL ude lje95 Lil yo4 ano 207 334 332 1421 30.1 AQs fooo 83 TTL 06/16/98 CTI 1081 90) 261 523 378 1424 29.0 AQS Food 84 1TL 06/24/98 CT! 1070 913 255 510 403 1424 27.9 AQS Food 85 TTL 07/07/98 C11 1066 924 249 498 425 1422 27.0 AQS Food 86 T"IL 08/10/98 CTI 972 927 241 482 445 1408 26.0 ACS rood 87 1TL 08/18/98 CTI 944 928 233 467 461 1394 25.2 AQS Food 88 TIL 08/2668 CII 740 917 231 461 456 1378 25.1 AQS Food 89 TIL 09/01/98 CII 810 912 225 451 461 1362 24.7 AQS Food 90 1TL 09/09/98 CII 1174 925 227 454 470 1379 24.6 AQS Food Nacl REFERENCE T0XICANT DATA Ceriodaphnia dubia 1000 . v. -ff '-y

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n$ - \\ E Twenty Most Recent Tests 1 1 1 Page 1 of 1

CHRONIC REFERNCE TOXICANT PEECORD SHEET Study: Reftox # @ Test Organism: C. dubia f-ff9/6-Of# i i i In tiat on Daterr me:- Termination Date/ Time: 9-is-ff/ofm o9f Control / Dilution Water: C-medium w/o EDTA: 2 7 6 't- , zM Personnel: pd 5 C An c-Toxicant: Nacl 1 j Source: Fisher Scientific Lot #: % i 1m Test Concentrations: Control. 0.6. 0 9.1.3.1.8. 2.6 c/L i Released From: (Date/ Time) 9.f-fr/2.2oo To:(Date/ Time) f f-f 8 /ocoa -icv: II,4 Notes: T 4 d v ~ r,. ..n-, y e--r 7

CERtODAPHNIA SURVIVAL AND REPRODUCTION TEST Smdy: Rcftov # 70_ Page 1 of 2 hitinim Dae: f f# Time: DP00-Of/J~ Termmaim Dae: 4-tT-T8 Time: rdoo tT14 Treatment By:

D CLic GkJL l DJ 7 071 l U36 l

l Total (Randomt7ntion #) Rep 1 2 3 4 5 l 6 7 { 8 l Yotmg us* 2 -7& Z 29&'2--,7762 1942. z ? t s* A.% 3 l l 1 n o b F R

14 29 2

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CERIODAPHNIA SL9WiVAL AND REPRODUCTION TEST Study-[<f or # 9 0 Page 2 of 2 Ininsum Dae:4.f-f 7 Tune: e90o -df/7 Temunaum Due: f-Ii-f f Tune: Ma e-ett o Treatment By. >TI I (## (< L'r D73 i M3 I i l l Total i 1 l 2 3 4 l 5 I 6 l 7 l 8 l Young (Randomization #) Reo W8 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 1 O O O 2. Wn ~ 2. 2 l } 2. LI n 6 1 3 t I I i 7 I 9 4 1 0 Wo o 18 t(5) 5 7_ T p/o (p 6 3 7 o 8 7 L f p } 8 / 1 0 3 9 j 1 t/ o n s 10 V V V 3 5 p/o g SI 4" JA M4 f/ W4 HJ UJ A/d

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h O 90c..-fr8 1 (-if - fr 9/A THE NUMBER OF RESAMPLES IS 80 7g j M os. n.Sg VN

- - LISTING OF GROUP CONCENTRATIONS ( % EFF. ) AND RESPGNSE MEANS ***

~ CONC. (%EFF) RESPONSE MEAN MEAN AFTER' POOLING .000 31.100 31.100 .600 29.900 29.900 .900 29.000 29.000 3.300 20.700 20.700 1.800 5.100 S.100 2.600 .000 .000 THE LINEAR INTERPOLATION ESTIMATE OF THE TOTAL IMPAC.T CONCENTRATION FROM'THE INPUT SAMPLE IS 1.1735. BOOTSTRAP PROCEDURE TO ESTIMATE VARIABILITY OF THE ESTIMATED ICp

- - - - xx***************

THE MEAN OF THE BOOTSTRAP ESTIMATES IS 1.1942. l THE STANDARD DEVIATION OF THE BOOTSTRAP ESTIMATES IS .0766. AN EMPIRICAL 95.0% CONFIDENCE INTERVAL FOR THE BOOTSTRAP ESTIMATE IS ( 1.0783. 1.3372 ). 4 I

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PERMITTEE NAME/ ADDRESS anciucie foc5ty Name/tocotion if CCet NATIONAL POLLUDON DISCHARGE EUMMAllON SYSTEM (NPDES) l OtSCHARGE MONITOR!NG REPORT (DMRJ r4anp_,Jg-jigguggtygggggggg - P.O. BOX 2000 IN0026450 103 G Low Vol. wasta Addess - - MEMU 5' - - - - - - - - '- heofmerit Pond l PERMIT NUMBER OtSCHARGE NO. _SO_DD_Y-DA_tSY._TN_.37._37_9_ _._ _ _ _ _ _ _ _ F_ocjty_ IVA-SEQUOYAH NUCt EAR PLANT Locotton HAMitTON COUNTY MnNITt'TMNC PfDinh YEAR MO DAY YEAR MO DAY ATTN: DEBBIE BODINE. ENV.MGR. FrOm: 98 09 01 TO: 98 09 30 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALY3'S AVERAGE MAXIMUM Uf4TS MNMUM AVERAGE MAXIMUM UreTS I METH MEASUREMENT <61.3 97.1 <5.6 8.1 0 5 / 30 GRAB ! Olt & GREASE FREON EXTR-GRAV SAMPLE 3 4GRAR:s $>.s ' /@.L JNOM MG/L %!q'p+ WI~ REOutREMENT ' q 90:00"0

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PERMITTEE NAME/ ADDRESS (include foc#ity Name/ location if cfffer NATIONAL POLLUTION DISCHAGGE EllMWATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT IDMRI _ M MUD W M M R _ _ Address P.O. BOX 2000 TN0026450 101 G oltruser Discharge - JNTEROFFIC.1SB 2A) p SODOY-DAISY, TN 37379 Factitty_ IVA SEQUOYAH NUCLEAR PLANT MON!TC DINC: PFRtOn tocotton HAM!LTONCOUNTY YEAR MO DAY YEAR MO DAY ATIN: DEBBIE BODINE. ENV. MGR. From: 98 08 01 TO: 98 08 31 1 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF 1YPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS 0 31 / 31 RCORDR FLOW,IN CONDUlf OR THRU SAMPLE - ~ * * - - ~~~ 165g ~*~~ TREATMENT PLANT MEASUREMEN 50050 "I

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MEASUREMENT MG/L (1/7.) (GRABf 00530 1 0 0 PERMIT Uh0 0001 I dNEI )NONE?^ 06.0055 REWREMENT fNONET N 101 <5.0 <5.0 0 4 / 31 GRAB OIL & GREASE FREON EXTR-GRAV SAMF1E ~~~ ~~~. ~~~~ METH MEASUREMENT MG/L 11/7-3 00556 1 0 0 PERMIT . ?NONEl OEddOI Dd!000I EGRABg I ggy REQUIREMENT 101 ggy 0.012 0 54 / 31 GRAB CHLORINE. TOTAL RESIDUAL SAMPLE MEASUREMENT MG/L QS/7f i_CALCU 50060 1 0 0 PERMIT ^ Lw. j ~ ~ REQUIREMENT j g 7 ', ) g g 101 NAME/11TLE PRINCIPAL EXECUTIVE OFFICER I CER!iFY UNDER PENALTY OF LAW THAT l HAVE PERSONALLY EXAMINED AND AM TELEPHONE DrTE FAMILIAA WITH THE NFORMATION SUBMITTED HEREIN AND BASED ON MY NQUIRY OF Masoud Bojestoni THOSE NDIVIDUALS IMMEDtATELY RESPONSIBLE FOR Oi1TAINING THE INFORMATION t BEuEVE THE SUBMITTED NFORMATION IS TRUE. ACCURATE AND COMPLETE. I AM AWARE n irnnm t 11 S perViSor 423 843-6700 98 09 16 Site ViCe President THAL lHERE ARE SIGNIFICANI PENALTIES FOR SUBM!TTING FALSE NFORMATION iGNATURE OF PRINCIPAL EXECullVE INCLUDING THE POSSIBILITY OF FINES AND IMPRISONMENT. SEE I8 U.S.C. 1001 AND 33 OFFICER OR AUTHORIZED AGENT EA NUMBER YEAR MO DAY TYPED OR PRINTED U.S C. 1319. (Pendties tsder these stahfes may bckJde fines (43 to $10.000 and or COMMENT AND EXPLANATION OF ANY VIOLATIONS T?eference o# ottochments here There was no Closed mode operation in August.1998. Injection of the phosphote-Copolymer blend. PCL-222. Into both trains of the ERCW system was begun on 8/31/98. Calculated Concentration of phosphate Oimit = or < 0.100 mg/L) was 0.008 mg/L EPA Form 3320-1 (Rev. 9-68) Previous edations may be used. (REPtACES EPA FORM T-40 WHICH MAY NOT BE USED) Page 1 of 7 l;

PERMITTEE ffAME/ ADDRESS - Onciude faciety Norrts/locotion if dfffer NATONAL POLLUfloN OtSCHARGE EUMWATON SYSTEM (NPDES) _Ngr!9_._ IV/dEQUDYfMiMLC.LEGR11&NL _ _ _ _ Adchess - P.O. BOX 2000 TN0026450 101 G oittuser otscharge PERMIT NUMBER DISCHARGE NO. - - - -SODDY-DAISY. TN 37379 Facilitt TVA-SEQUOYAH NUCLEAR PLANT tocotkn HAMfLION COUNTY MONITC DINC PFDinn p YEAR MO DAY YEAR MO DAY ATTN: DEBBIE BODINE. ENV. MGR. FrOm: 98 08 01 To: 98 08 31 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS j 0 31 / 31 RCORDR FLOW,1N CONDUli OR THRU SAMPLE 1658 1REATMENT PLANT MEASUREMENT ~ 50050 1 0 0 PERMIT MGD ~ LCONTs RCOROR l

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" ' ~ ' {GRABd ~ MG/L (1/7/ ' '~ 00556 1 0 0 PERMIT , g g jg REQUIREMENT 3 g ~.; x 10; I 0.012 0 54 / 31 GRAB CHLORINE. TOTAL RESIDUAL SAMPLE l MEASUREMENT i ga [ cal.Ch [ MG/L J/71 50060 1 0 0 PERMIT ! dNd INDNs E0 W REQUIREMENT 101 $ONEG INONEY N TELEPtONE DATE i NAME/ TITLE PRINCIPAL EXECUTIVE OFFICER I CERilFY UNDER PENALTY OF LAW THAI i HAVE PERSONALLY EXAMWED AND AM i FAM10AR WITH THE NFORMATION SUINITTED HEREW AND ELASED ON MY WQUIRY OF Mosoud Bojestani TwosE NOlviDUALS IMMEDIATELY REsPOt4SIBLE FOR ObfANING THE NFORMA!ON I BEUEVE IHE SUOMITIED WFORMATIONis TRUE. ACCURATE AND COMPLETE. t AM AWARE Enviro. ental Supervisor 423 843 4700 98 10 09 I Site Vice President THAT THERE ARE SIGNGICANT PENAlilES FOR $UBMITING FALSE NFORMATON SIGNATURE OF PRtNCtPAL EXECul1VE l INCLUDtNG THE POSSIEdUTY OF FNES AND tMPRISONMENT. $EE 18 UAC. 1001 Ato 33 OFFICER OR AUTHORIZED AGENT NUMBER YEAR MO DAY TYPED OR PRINTED u s c. 1319. (Pendtes trou these stddy may include ruws m to $10.000 cnd o, j [ COMMENT AND EXPLANATION OF ANY VIOLATIONS Reference of ottochmentshere There was no closed mode operation in August,1998. Injection of the phosphate-copolymer blond. PCL-222. Into both trains of the ERCW system was begun on 8/31/98. Colculated [ L concentsotton of phosphoto (limit = or < 0.100 mg/L) was 0 007 mg/L EPA Form 33201 (Rev. 9-88) Previous edifsons may be used (REPLACES EPA FORM T-40 WHICH MAY NOT BE USED) Poge 1 of 7 l l

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PERMITTEE NAME/ADORESS (7nclude fac&ty Nants/Lccation if dffer NAI:ONAL POLLUTON EMSCHARGE ELENATION SYSTEM (Nf' DES) DISCHARGE MONITORING REPORT (DMR) _ M MUM MCM M L __ Address P.O. BOX 2000 IN0026450 103 G Low Vof Woste Treatment Pond U" PERMIT NUMBER DISCHARGE NO. SODDY-DAISY. TN 37379 Facly_ IVA-SEQUOYAH NUCI EAR PLANT HAMitTON COUNTY MONITC DINC: PFRIOD Locahon YEAR MO DAY YEAR MO DAY ATIN: DEBB!E BODINE. ENV. MGR. FrOm: 98 08 01 TO: 98 08 31 [ QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY 5 AMPLE PARAMETER EX OF TYPE ANALYS'S AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNIIS METH MEASUREMENT <35.7 <53.9 <5.0 <5.0 0 4 / 31 GRAB Olt & GREASE FREON EXTR-GRAV SAMPLE MG/L M/7 e 4 RABs 00556 1 ~ 0 0 PERMIT LBS/ DAY 103 [i905004 f2503)D0 NONET il5.0001 J20L000 ~ REQUIREMENT ~ 55 178.58 7.25 21 0 4 / 31 GRAB SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT MG/L %1/71 dGRABA 00530 - 1 0 0 PERM:T LBS/ DAY REWREMENT 103 !380 00'0? 1250.000? 5 NONEt f30t 1100* ^ 0 31 / 31 RECROR FLOW,IM CONDUlf OR THRU SAMPLE 0.851 1.642 TREATMENT PLANT MEASUREMENT 50050 1 0 0 PERMIT MGD M/l RECRDR s N' NE4 REQUIREMENT ENONEi NONEV VNONE' JNONE6 O 103 7.53 8.37 O 11 / 31 GRAB PH SAMPLE MEASUREMENT PH UNIT c3/7 ; ? GRAB; + 00400 1 0 0 PERMtI IEWREMENT TNONEE NONE$ F6.0 t NONEL @.0i 103 SAMPLE MEASUREMENT PERM 1T REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERM lT REQUIREMENT NAME/ TITLE PRINCIPAL EXECUllVE OFflCER I CERfifY UNDER PENALTY OF LAW IHAIl HAVE PERSONALLY EXAMINED AND AM TELEPHONE DATE FAMitIAR W!!H THE INFOQMAflON SUBMiTIED HEREIN AND BASED ON MY INQUIRY Of fj M MaSoud Bojestoni IHOsE NOlVIDUALS IMMEDIATELY DESPONSIEkE FOR OBI AINING IHE INFORMATION i BEufvE THE SUBMITIED tNFORMAllON ISIRUE, ACCURATE AND COMPLETE. l AM AWARE E viron. ental Supervisor 423 B43-6700 98 09 14 Site ViCo President IHAT IHERE ARE SIGNIFICAN1 PENALTIES FOR SUBMIT 11NG FALSE NIORMAllON SlGNATURE OF PRINCIPAL EXECUTIVE INCLLONG THE POSStuluTY OF INES AND IMPRISONMENY. SEE 18 U S.C. tool AND S3 OFFICER OR AUTHORilED AGENT EA NUMBER YEAR MO DAY U S C. 1319 (Penctnes under twse stcsues tnoy incbde rnws tp io s to. coo u w1 c, TYPED OR PRINTED COMMENT AND EXPLANATION OF ANY VIOLAllONS 7?eference o#ottochments here No Comments. EPA Form 3320-1 (Rev. 9-88) Previous edhons nuy be used (REPLACES EPA FORM T-40 WIOCH MAY NOT BE USED) Page 3 of 7

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FERMITTEE NAME/ ADDRESS - (lttcAxfe Focctr Noots/locorion # cAfter NATIONAL POLLuflON DECHARGE ELOtlNAYlON SYS!EM (ffDES) DISCHARGE MONITORING REPORT (DMRI % g ggg g g g Address P.O. BOX 2000 TNn026450 112 G sewage Treatment _OriLEROfflCE SB_2A)- ------ -- NUU# PERMIT NUMBER DISCHARGE NO. SODOY-DAISY. TN 37379 Focji tL TVA-SEQUOYAH NUCLEAR PLANT _ t Locatk>n HAMILTON COUNTY MONITC RINC; PFDIOO YEAR MO DAY YEAR MO DAY ATIN: DEBBIE BODINE.ENV. MGR. From: 98 07 01 To: 98 07 31 PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINMUM AVERAGE MAXIMUM UNITS ANALY3'S BOD, S-DAY (20 DEG. C) SAMPLE MEASUREMENT 00310 T0 0 PERMif LBS/DA MG/L [2/309 (GRAB [ 4 112 [ggg jgg g g ggg; ): g g f s REQUIREMENT SOLIDS. TOTAL SUSPENDED SAMPLE MEASUREMENT 00530 1 0 0 PERMIT LBS/DA MG/L ?2/30 ii / GRAB 4 I dNE7 1 30.0 07 N50dO2 [ REQUIREMENT 112 E'l SNdNEE N FLOW,IN CONDUli OR THRU SAMPLE TREATMENT PLAN; MEASUREMENT L CONT 2 RECRDR. 50050 1 0 0 PERMIT MGD REQUIREMENT 1 'EY M NE INONEi ?i 'DNEP 4NO $3 I12 Solids, $ETTLEABLE . SAMPLE MEASUREMENT ML/L 22/71 (GRABW 00545 1 0 0 PERM 4T $LOUO$ ^ REQUIREMENT SONE 4 f NONEV ' NUNES J NONE3 1 112 COLIFORM. FECAL SAMPLE MEASUREMENT 31616 1 0 0 PERMIT N/100 M 'V2/301)

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REQUIREMENT fNONN UN'ONE9 NONEl 7200.00M "l'dO0iO004 H2 CHLORINE. TOTAL RESIDUAL SAMPLE MEASUREMENT A 50060 1 0 0 PERMIT MG/L $5/7-i iGRABi 112 gg ; N'ONEi i NONES 4NONEU REQUIREMENT s E.000i i 2 SAMPLE MEASUREMENT PERMIT ^ REQUtREMENT NAME/ TITLE PRINCIPAL EXECUTIVE OFFICER I CEQTdY UNDER PENALTY OF LAW IHAl I HAVE PERSONARY EXAM:NED AND AM TELEPHONE DATE l F AMitIAR WITH IHE NFORMAIlON SUBMilllD HEREIN AND BASED ON MY INQUlrW OF Masoud Bolestani IHO5E INDIVIDUALS IMMEDIATELY DESPONSIBLE FOR OBTAINING THE INFORMAflON I BEUEVE THE SUBMIITED INFORMAllONIS TRUE, ACCURATE ANO COMPLETE. l AM AWARE En to ental Supervisor 423 843-6700 98 '08 12 Site Vice President IHAT IHERE AreE SIGNIFICANT PENALilES FOR SUBMilllNG FALSE INFORMATION j SIGNATURE OF PRtNCIPAL EXECUTIVE INCLUDING THE POSSIBluTY OF FNES AND IMPRISONMEN! SEE I8 0 sC. 1001 AND 33 OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY UsC 1310 (Perdtws mder lhese stc*ses troy nclude inws m to $10,000 and a, TYPED OR PRINTED COMMENT AND EXPLANATION OF ANY VIOLATIONS Reference o# ottochmentshers t SeWoge hos been diverted trom this system since January,1998. DSN 112 is maintained in stond by status. There is flow tryough the System. EPA Forrn 3320-1 (Rev. 9-85) Previous editons rnoy be used (REPLACES EPA FORM T-40 WHICH MAY NOT BE USED) Page 6 of 7

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