ML20081C221
| ML20081C221 | |
| Person / Time | |
|---|---|
| Site: | Satsop |
| Issue date: | 09/30/1983 |
| From: | Davis W, Jeane G, Northstrom T WASHINGTON PUBLIC POWER SUPPLY SYSTEM |
| To: | |
| Shared Package | |
| ML20081C212 | List: |
| References | |
| NUDOCS 8310310154 | |
| Download: ML20081C221 (290) | |
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!. ENCLOSURE 2 l SUPPLY SYSTEM NUCLEAR PROJECT NO. 3 l
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t REPORT ON FISH BI0 ASSAYS (0 RAFT)
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' 8310310154 831021 PDR ADOCK 05000500 l A PM l - _ _ __
TOXICITY OF COPPER, ZINC, AND THEIR CHEMICAL FORMS TO COHO SALMON AND STEELHEAD TROUT IN THE CHEHALIS RIVER, WASHINGTON l l
"EiQt Wallace Davis, III Terry E. Northstrom G. Scott Jeane James E. Mudge WASHINGTON PUBLIC POWER SUPPLY SYSTEM September 1983
EXECUTIVE
SUMMARY
In 1974 the Washington Public Power Supply System submitted Federal and State siting applications for two nuclear power projects (WNP-3 and WNP-5) to be located on the Chehalis River (near river mile 21) in Grays Harbor County, Washington.
Construction (which commenced in 1977) and operation of the projects were conditioned by a National Pollutant Discharge Elimination System (NPDES) Permit which was issued by the Energy Facility Site Evaluation Council (EFSEC) in October 1976 and modified in October 1979. Hearings on the initial permit and the Supply System's subsequent modification request focused on anticipated metal content, notably copper and zinc, in the discharge and the postulated effects of such metals on important anadromous fish in the Chehalis River. Recognizing considerable uncertainty, as manifest by differing professional opinions -
offered in hearing testimony, as to the toxicity of the discharge, EFSEC included in the modified permit a requirement that site specific, flow-through bioassays be performed on local salmonids to assess the toxic levels of copper and zinc, both singly and in combination, during different times of the year and with dif.ferent life stages.
The work of numerous investigators indicates that many factors affect the toxicity of metals to fish. Some of the chemical factors which appear to affect copper and zine toxicity are: pH, hardness, alkalinity, temperature, chemical form of the metal, and organic and inorganic complexing capacity of the water.
Biological factors such as species sensitivity, size, and/or life stage have also been reported to play a role.
To develop site-specific data responsive to the NPDES Permit requirement, bioassays were conducted by the Supply System on the Chehalis River (near river mile 19) from February into December 1982. The tests were performed utilizing ambient water from the Chehalis River and a flow-through serial diluter system.
Chehalis River strains of fry, parr, and smolt of coho salmon and steelhead trout were used in the tests.
The principal objective of the test was to determine what concentrations of copper and zine would result in no observable long-term effects (measured at 60 days). Secondary objectives were to obtain short-term and long-term LC50s, to determine whether the two metals were more or less toxic in combination than singly, and to monitor the water quality parameters which may affect toxicity.
We observed that copper and zinc toxicity was seasonal in nature and that acute copper toxicity, when based on the labile form only, was correlated with several water quality parameters.
Temperature and the ability of the river to " bind" copper were particularly important in determining the toxic effects of copper beyond 96 hours0.00111 days <br />0.0267 hours <br />1.587302e-4 weeks <br />3.6528e-5 months <br />. Usually, mortality was a better indicator of long-term toxic effects than was growth. The long-term, no-offect level for coho was 18 ug/l total copper or 220 uq/1 total zinc; the respective concentrations for steelhead are 24 uq/1 and 59 ug/1.
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By comparing all of the 96-hour and 9-day two-metal results, it appears that the toxic interaction effect (additive, synergistic, or antagonistic). is a function of time, and possibly, the relative concentration of the two metals. when zine is present in high concentrations relative to copper, the interaction effect may be synergistic. As the relative proportion of cooper increases (total concentration of zinc decreases), the effect becomes additive, and as time increases so that the concentrations decrease, there is an indication of antagonistic interaction. At the relative' concentration of copper and zine which are expected to be discharged from imp-3, the interaction effect. will be either additive or antagonistic, clearly not synergistic.
It also appears that labile copper may be preferable to total copper as an indicator of toxicity. This is not the case with labile zinc or either of the dissolved forms of the metals.
Labile copper may also possess the additional advantage of being an integrator of water quality factors and the data may, therefore, be more directly applicable to different water systems.
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TABLE OF CONTENTS .
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Introduction 1 Materi~als and Methods 3 General 3 Acute Tests 4 Subacute Tests 5 Chemical Methods 5 Results and Discussion 9 General Chemistry Conditions 9 96-Hour LC50 Tests (Total Metal) 10 Long-term LC50 Tests (Total Metal) 11 Subacute Tests (Total fietal) 12 Metal Speciation and Water Quality 14
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Bibliography 18 Quality Control and Standard Conditions A-1 Metal Concentration Data B-1 Water Chemistry Data C-1 Summary of Bioassays by Project D-1 Spearman-Karber Calculations E-1
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INTRODUCTION In 1974 the Washington Public Power Supply System submitted Federal and State siting applications for two nuclear power projects (WNP-3 and WNP-5) to be located on the Chehalis River (near river mile 21) in Grays Harbor County, Washington.
Construction (which was commenced in 1977) and operation of the projects were conditioned by a National Pollutant Discharge Elimination System (NPDES) Permit which was issued by the Energy Facility' Site Evaluation Council (EFSEC) in October 1976 and modified in October 1979. Hearings on the initial permit and the Supply System's subsequent modification request focused on anticipated metal content, notably copper and zinc, in the discharge and the postulated effects of such metals on important anadromous fish in the Chehalis River. Recognizing considerable uncertainty, as manifest by differing professional opinions offered in hearing testimony, as to the toxicity of the discharge, EFSEC included in the modified permit a requirement that site specific, flow-through bioassays be performed on local salmonids to assess, the toxic levels of copper and zinc, both singly and'in combination, during different times of c.he year and with different life stages.
The work of numerous investigators indicates that many factors affect the toxicity of metals to fish. Some of the chemical factors which appear to affect copper and zinc toxicity are: pH, hardness, alkalinity, temperature, chemical form of the metal, and organic and inorganic complexing capacity of the water.
Biological factors such as species sensitivity, size, and/or life stage have also been reported to play a role, (e.g. Hodson et al, 1979; Spear and Pierce, 1979; Howarth and Sprague, 1978; Fogels and Sprague, 1977; Chakoumakes et al, 1979; Chapman and McCrady, 1977).
Available acute copper and zine toxicity data (96h LC50s) for freshwater fish shows a wide range of values - 10 to 10,200 ug/l for copper and 90 to 40,900 ug/l for zinc. In comparison, the subacute copper and zine data bases, which are smaller, show a relatively narrow range of values - 3.9 to 60.4 ug/l for copper and 47 to 852 ug/l for zinc (USEPA, 1980a,b).
To develop site-specific data responsive to the NPDES Permit requirement, bioassays were conducted by the Supply System on the Chehalis River (near river mile 19) from February into December 1982 _with steelhead trout (Salmo gairdneri), and coho salmon (Oncorhynchus kisutch). Life stages tested included fry, parr, and smolt. In an effort to more fully understand the factors which affect toxicity, a wide range of water quality parameters were measured, including various species of copper and zinc.
Increased interest in metal ion speciation has arisen largely because of the recent discovery by many workers that metal ions, including copper, appear to be less toxic or biologically unavailable when they are complexed strongly with humic 1
oubstancca. Bscouse of this and other roles in the mobility of motel ione in natural water systems, humic substances have baan referred to as metal-ion buffers. The limit of a natural water ,
system under a given set
- of environmental conditions to complex a l' metal is referred to as the complexation capacity. The term labile metal refers to that portion of the total metal which has been demonstrated, utilizing a particular analytical technique, to be uncomplexed or weakly so. Specifically, herein, labile metal includes free metal ions plus those complexed metal ions which can be disassociated during the analysis to yield free or 1 electroactive forms of the metal. Non l'abile metals thus would i include those metals which are bound in organic or inorganic l complexes or adsorbed onto suspended or colloidal matter.
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MATERIALS AND METHODS General .
l All procedures met, as a minimum, the requirements set forth in Standard Methods for the Examination of Water and Waste Water
( A PHA , 1980). In order to ensure consistency of methodology throughout the program, a detailed procedure manual was prepared and followed by all members of the team. In this report, the term " acute" refers to tests of ten days duration or less, while
" subacute" refers to tests of 30-60 days. In some cases, it was possible to calculate short-term LC30s from subacute tests.
Long-term LC50s which we present are analagous to the term ILL or incipient lethal limit; we generally do not use the term ILL.
i because long-term LC50s are presented for several different test durations.
Water for the tests was drawn directly from the Chehalis River with a submersible pump covered with a 1/8" wedge wire screen.
Because the primary goal of the study was to assess the effects of copper and zine in natural river water, no effort was made to treat the water or moderate physical characteristics such as temperature. Recognizing that debris would cause plugging of the small diameter tubes in the diluters, an initial effort was made to eliminate large material with a commercial centrifugal i
separator. This, however, proved to be inadeauate and it was necessary to add a coarse (0.5mm), in-line bag filter to remove sand, twigs, and leaf fragments.
Tests were conducted in 110 liter glass acuaria maintained at a volume of approximately 100 liters. Serial diluters similar to those described by Garton (1980) were utilized to provide one liter flow / minute / aquaria. Each of the four diluters provided flow to two pairs of six aquaria (one control and five different toxicant concentrations -
Figures 1 and 2). This flow provided more than 50% replacement of water each hour.
Prior to each test, stock solutions of zine nitrate and/or copper
. nitrate concentrate were prepared. This concentrate was then
- volumetrically mixed every 1-2 days with deionized water (approximately 16-18 megohms / square centemeter) and sufficient nitric acid to provide a pH of approximately 2. The mixture was then pumped to a small head box and delivered to the diluter through a small tube at a flow rate of approximately 15-20 ml/ min.
Photoperiod was adjusted at least every two weeks to correspond with local day length. Two banks of fluorescent lights were set on different timers so that there was a transition period of 15-30 minutes between full light and dark.
In this report, the term " Project" refers to a series of tes's t which were run concurrently. Projects 1 and 2 were rangefinding tests conducted in December 1981 and January 1982. The results 3
of these tests are not presented here.
DRAFT With the following i
Dr exceptions, Projects 3-8 were run to completion utilizing all ((
four diluters (48 acuaria): Two diluters failed prior to the end EE of Project 3, one diluter was started late in Project 4, and in ir Project 6, while all diluters were started concurrently, two were used for subacute tests and two were used for acute tests.
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e Fry, parr, and smolt life stages of coho salmon and steelhead E5 used in the toxicity tests. The size of the fish by "
trout were bioassay project is presented in Table 1. All fish were provided -
by the State of Washington from nearby hatcheries; steelhead were g provided by the Game Department and the Department of Fisheries my provided coho. Fish were acclimatized in large holding tanks for Z.
at least two weeks prior to testing. A commercial fish food 5 (Oregon Moist) was utilized with food size and feeding rates as F used at the hatcheries from which the fish were obtained. Fish E were not fed for 48 hours5.555556e-4 days <br />0.0133 hours <br />7.936508e-5 weeks <br />1.8264e-5 months <br /> prior to the start of, or during acute :-
bioassays. Fish were also not fed within 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> of f anesthetization. One analysis was conducted on the fish food for E copper and zinc content.
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Prior to each test, equal numbers of fish were distributed in a $
stratified random manner to the test aquaria. Loading factors k ranged from 0.4 to 9.3 grams per liter of aquarium volume or from E 0.03 to 0.64 grams / liter / day based on flow rates. These loading =
factors are within recommended limits for flow through toxicity ;
tests (Committee on Methods for Toxicity Tests with Acuatic g Organisms, 1975). A minimum of 48 hours5.555556e-4 days <br />0.0133 hours <br />7.936508e-5 weeks <br />1.8264e-5 months <br /> acclimatization period 7 was provided prior to the introduction of toxicant to the water =
flow.
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E Acute Tests Acute tests were started by drawing down the water in the aquaria depending on the size of the fish, and then
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1-3 cm depth, to allowing them to fill up at the standard flow rate of 5 approximately 1 liter / minute. Checks for dead fish were made at -
least twice per day for the first 96 hours0.00111 days <br />0.0267 hours <br />1.587302e-4 weeks <br />3.6528e-5 months <br /> and at least daily ,
thereafter; dead fish were removed when observed.
E The first copper and zinc two-metal test (Project 4) was conducted with concentrations of the two metals present in roughly the same . proportion as the ratio of the single metal -
LC50s observed in Project 3. Subsequently, it was decided that more useful information, from a regulatory point of view, would I
be obtained if the metal ratio more nearly represented that which would be found in the power plant discharge. Accordingly, all other two-metal tests, both acute and subacute, utilized a high copper:zine ratio of approximately 1:3.
All LC50 calculations were pe rf orn.ed utilizing the trimmed Spearman-Karber methodology (Hamilton et al, 1977). A key advantage of this method is that it never fails, even if unusual mortality patterns are observed. In many of our tests, probit 4
CRAFT analysis would not work because of low observed mortalities.
Wherever possible, we utilized a 10% trim. When it was not possible to utilize a 10% trim due to low mortalities, higher trims (maximum of 30%) were used. A sensitivity analysis performed on those cases where it was possible to calculate the LC50 over a wide range of trims indicated that % trim had little effect on the final estimate although the confidence limits do expand with higher percents. This technique was applied to all time intervals.
Subacute Tests During subacute bioassays, toxicant flow was initiated without drawing the volume of the tank down. The flow of toxicant from the diluter into the tank resulted in a 90% replacement of the raw river water in approximately 3.5 hours5.787037e-5 days <br />0.00139 hours <br />8.267196e-6 weeks <br />1.9025e-6 months <br />. All fish were anesthetized and weighed (to 0.lg) when placed into the aquaria and approximately every 10 days thereaftar. Length measurements (to 1.0mm) were also collected every 10 days during Project 5, but only every 30 days during Projects 6 and 8.
Means and standard deviations were calculated for weight and length by tank. Statistical techniques applied in order to determine which concentrations resulted in a significant effect included analysis of variance and covariance, a posteriori tests (Student-Newman-Keuls and Duncan's Multiple Range test - both at the 5% significance level), and comparison of regression line slopes with the t-test. The binomial distribution (5%
significance level) was utilized to assess non-random mortality.
Prior to all of the projects, the condition of the fish was examined by a pathologist from the Washington Department of Fisheries. On several occasions tests were delayed until either new fish could be obtained or the fish were declared healthy. At the end of the last subacute bioassay, Project 8, five fish from each of the 48 tanks were examined quantitatively for condition.
Skin, gill, liver, and kidney tissue from each fish were individually graded based on gross anatomical appearence from 1.0 to 5.0; 1.0 represented very poor condition, 3.0 represented an average hatchery fish, and 5.0 represented a tissue in " perfect" condition. In addition, the total number of parasites for each
, fish was recorded prior to examination. The pathologist was unaware of the treatment concentration of the fish he examined.
The average condition of each species-tissue-concentration combination was determined.
Chemical Methods water quality sampling parameters, frequencies, and locations are presented in Table 2; analyses were performed per USEPA (1979).
In addition, metal samples were collected at some of the diluter discharges to compare concentrations entering the aquaria with concentrations measured in the aauaria. The primary purpose of g
DRAFT this measurement was to help assess whether or not the diluters were functioning properly, and to identify which samples from the acuaria had analyzed metal concentrations that were highly influenced by food or feces. Water samples were drawn from the center of the aquaria with a 50 m1 syringe to which had been attached a short length of plastic tubing. One syringe was dedicated to each tank throughout an experiment. In addition to the laboratory analysis of water samples, temperature, D.O., pH, and conductivity were collected daily in the river, one head box, and in each diluter by probe. The same four parameters were recorded nearly continuously on strip charts from probes in the water line from the river and in one control tank.
An extensive quality control program was an important part of all projects. Participation in EPA 's round robin program, use of EPA and/or NBS controls, replicate analyses, and measurement of contamination in new or acid washed bottles were part of the quality control program. Results of this program, including precision an accuracy of chemical parameters, are presented in Appendix A.
All total and dissolved metal samples were analyzed on a Perkin-Elmer Model 4000 atomic absorption spectrophotometer equipped with an HGA 400 graphite furnace, an AS40 auto sampler and an EDL power supply. All chromium, lead, cadmium, nickel, zinc <25 ug/1, and copper <100 ug/l samples were analyzed utilizing the graphite furnace technique. For all graphite furnace analyses, the carrier gas utilized was certified grade 5 argon at 40 ml/ minute. Samples for calcium, magnesium, sodium, potassium and iron were analyzed by flame atomic absorption spectrophotometry utilizing an air acetylene flame.
A flow spoiler was used when aspirating all Flame AA samples except copper, iron and zine which were <0.2 mg/l wherein a glass bead was substituted for the flow spoiler. Atomic absorption methods utilized were modified from EPA Methods for Chemial Analysis of Water & Wastes (1979). Graphite furnace samples were analyzed in groups of twelve samples. Each group, not including calibration standards, included 1) a reagent blank, 2) a standard, 3) a duplicate sample, 4) a certified NES or EPA reference control.
Six replicates were averaged for each flame sample whereas all graphite furnace samples were run in duplicate. Standard conditions for flame and graphite furnace analyses are presented in Appendix A.
Laboratory water was prepared from tap water by reverse osmosis followed by recirculation through a Barnstead Nanopure 4-module system consisting of two mixed-bed ion exchange cartridges, one activated carbon organic and chlorine removal cartridge, and.a 0.1 um final filter cartridge. Specific resistance was maintained between 15 and 18 megohms / centimeter. All metal samples were acidified to 0.5% with Ultrex nitric acid unless specified otherwise.
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For the UNM T determination of dissolved n atals, unacidified samples were filtered through 0.45 um membrane filters immediately after collection. Polysulfone filter funnels with metricel membrane filters were used threughout the bioassay. The first 50 ml of filtrate was discarded and the subsequent 75 ml of filtrate was collected and acidified to 0.5% with nitric acid.
Measurements of labile copper or zine were obtained with a Princeton Applied Research Model 384 polarographic analyzer, model 303 hanging mercury drop electrode and a Houston Omniplot digital plotter. A Ag/AgCl reference electrode and a platinum
) wire counter electrode was used in teflon cells. Oxygen was removed by purging with grade 5 ultrapure nitrogen which had been passed through Messer Griesheim Oxisorb cartridges. All measurements were made at 20 degrees C and at the ambient pH of the water sample. The measurements were performed by titrating in duplicate a 10 ml volume of sample with copper or zine ion I solutions of known concentrations, usually 10 mg/1. pH values were adjusted to ambient when necessary, with ultrex nitric acid or KOH. The supporting electrolyte was 0.lM KNO3 purified by passage several times through chelex 100 resin. The standard conditions for all copper and zinc DPASV (differential pulse anodic stripping voltametry) measurements are presented in Appendix A.
Labile copper and zine analyses were performed using the method of standard additions. A voltammogram of the unspiked sample was recorded, then a known volume of metal ion solution was added which was sufficient to approximately doule the original peak height. The slope determined by the standard additions to the sample was used as the calibration slope and the concentration of labile copper or zine was determined by extrapolating the calibration line backward until it intercepted the concentration axis. Complexing capacity measurements were made by initially spiking the sample with a known volume of metal ion solution I
sufficient enough to surpass the metal-ligand ratio of 1:1. This was followed by successive additions of standard solution which would approximately double the peak current. The complexing l capacity was then calculated using a least squares regression of peak current versus incremental copper concentration.
Based on a visual inspection of the samples, it appeared that a significant contributor to variability in the concentration of metal in a sample was contamination from particles, particularly I
food and fecal material, which were present during the subacute l tests.' In an effort to eliminate outliers, prior to determining the average metal concentration in a tank, all samples were put through a statistical selve as follows:
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- 1) The mean and standard deviation of the entire data set DRAFT was calculated.
- 2) All samples who'se values deviated from the mean by more than two standard deviations were eliminated.
- 3) A new mean and standard deviation was calculated on the remaining samples and step 2) was repeated. The process was continued until all values utilized to calculate the average concentration were within two standard deviations of the mean.
In general, the problem of contamination resulted in a data set which was skewed to the right. The cleaning process eliminated very few values which were less than the final mean and nearly always reduced the average from that calculated by utilizing only the raw data.
Inspection of the copper and zine speciation data revealed that there was an obvious relationshio between labile or dissolved metal and the total metal. As water quality appeared to affect this relationship, percentages (i.e. labile or dissolved as a portion of total) or regressions (as appropriate) were calculated for each project.
For all other physical / chemical measurements, statistics were limited to means and standard deviations.
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RESULTS AND DISCUSSION General Chemistry Condit, ions The Chehalis is a small coastal river draining west-central Washington. The annual mean flow near the WNP-3 diffuser is 6630 cubic feet per second (cfs). The monthly mean ranges from 730 cfs in August to 14,865 cfs in January. The flow in the river is quite variable and reflects the seasonal rainfall distribution.
In general, the quality of the river at the site is considered good. Generally, the water is relatively soft (hardness of 21-36 mg/1), has low dissolved solids, high dissolved oxygen concentration (above 8.0 mg/l), and a pH which ranges from 6.5 to 7.6. River water temperatures range from a monthly mean of 5.6 degrees C in January to 18.3 degrees C in August.
The ambient water quality of the Chehalis River is summarized for each of the projects in Table 3, and the annual average concentration of trace metals, except copper and zinc, is presented in Table 4. All available data on Chehalis River flow, temperature, dissolved oxygen, pH, conductivity, and copper binding capacity during Projects 3-8 is presented in Fiaures 3 through 8. Data collected during this bioassay program is similar to historical data for the Chehalis River (Washington Public Power Supply System, 1982) and other pacific northwest streams (Samuelson, 1976).
A comparison of basic water quality parameters (temperature, D.O., pH, and conductivity) between the river and the aquaria shows a generally close agreement for all parameters.
Temperature difference was almost always less than 1.0 degree C with the aquaria being slightly warmer; the highest recorded difference was +1.2 degrees C during Project 3. Depression of D.O. in the aquaria was typically <1 mg/l and only on rare occasions did it drop below 8mg/1. The extreme value was a 3.3 mg/l depression of D.O. to a value of 6.9 mg/l on one measurement in Project 5. The pH was generally ~0.2 units lower in the toxicant aquaria than in the river. During Project 6, there were a- few occasions when the river was 0.6 pH units higher than the aquaria. Conductivity was generally <5 umohs lower in the aquaria; thn in the river; however, on occasion, the difference was as high as 21 umohs.
f Water quality conditions in the aquaria are presented for each of the . projects along with summary biological information in Appendix D. Data on ambient as well as test concentrations of copper and zinc are presented in Appendix B for all metal species measured. The ambient concentrations of copper and zine were generally less than 2 and 3 ug/l respectively. The concentrations for labile and dissolved metal were estimated from the equations presented on these tables.
The food which was fed to the fish contained approximately 12 ug/gm total copper and 190 ug/gm total zine based on one analysis. Details are presented in Appendix B.
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96-Hour LC50 Tests (Total Metal)
The results of all 96-hour LC50 tests are summarized in Table 5.
The values for total metal LC50 for copper were more variable for coho salmon than for steelhead. The range for coho was61-103 ug/l while that for steelhead was 90 to 94 ug/1. Coho fry and smolt tested in May-June (Project 6) produced the lowest values which we observed (61 and 63 ug/l respectively). Our values for Coho agree with those of Lorz and McPherson (1976) and are slightly higher than the 46 ug/l reported by Chapman and Stevens (1978). Our coho values are 2 to 4 times higher than those reported for Chinook salmon (Oncorhynchus tschawytscha) by Chapman (1978a) and Finlayson and Verrue (1980, 1982). The 96-hour LC50 values for steelhead with copper are 2 to 3 times higher than those reported by Chapman (1978a), Howarth and Sprague (1978) and Finlayson and Ashuckian (1979). We believe that most of the inter-study variation results from differences in water cuality while (e.g. copper complexing capacity) species / variety factors also are a factor. In agreement with Chapman (1978a), there appears to be little effect of life stage on susceptibility to copper.
The measured 96-hour LC50 values (Table 5) for zine were significantly lower for steelhead (406-688 ug/1) than for coho (1900-2022 ug/1). Our zinc results for steelhead are similar to those reported by Sinley, et al. (1974), Nehring and Goettl (1974), Goettl, et al. (1974), and Holcombe and Andrew (1978) for rainbow trout (Salmo gairdneri) in water with a hardness comparable to that of this study. Our coho results for zinc are between those of Chapman and Stevens (1978; 905 ug/1) and Lorz and McPherson (1976; 4600 ug/1).
When coho were tested with both copper and zinc present in roughly the ratio of their single metal LC50's (i.e. Cu:Zn ratio of ~l:20, Table 5, Projects 3 and 4), the resultant mortalities indicate a slight synergistic effect when utilizing the technique of Marking and Dawson (1975). This agrees with Sprague's (1964) work with Atlantic salmon (Salmo salar) and Lloyd's (1961) studies on rainbow trout at copper.:zine ratios of 1:11 and 1:6, respectively, and high concentraions. The LC50 of the mixture (36 ug/l copper and 754 ug/l zine) was comprised of 0.40 of the concurrently run zine alone LC50 and 0.42 of the copper alone LC50. Because there appeared to be a significant difference between the susceptibility of steelhead to zine between Project 3 when the copper /zine ratio was determined, and Project 4, it is not possible to determine the interaction effect of copper and zine in combination (antagonistic, additive, or synergistic).
Statistically, there was no difference between the LC50 of zinc alone (406 ug/1) and zine in combination with copper (389 ug/l zinc). As a result, it is concluded that, at the concentrations tested (copper concentration less than 1/8th that of zine), the role of copper in the mortality of steelhead was small.
Additionally, at that ratio, th e're is not a synergistic interaction effect.
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DRAFT Subsequent acute tests with copper and zinc in combination were conducted with relative concentrations of the two metals more nearly approximating those which are expected in the discharge of the power plant (i.e. Cu:Zn ratio of ~1:3). In all cases where the two-metal LC50 may be directly compared with the one-metal LC50 (Projects 6, 6A, and 7 with coho, and Project 7 with steelhead), there was a reduction in the LC50 of copper when zine was present. The amount of this reduction ranged from 6 to 21 ug/1. The lowest obs'erved two-metal LC50 values were 55 ug/l copper and 160 ug/l zinc for coho and 45 ug/l copper and 128 ug/l zinc for steelhead. These values occurred concurrently with the lowest observed copper LC50 (61 ug/1) for coho and are the subject of additional discussion later in this report. It appears that the copper /zine interaction is either additive or antagonistic in nature when the Cu:Zn ratios are ~1:3. This observation is consistent with the findings of Finlayson and Verrue (1982) for chinook salmon and Lloyds's (1961) rainbow trout results at low concentrations of copper and zinc. Our 96-hour LC50 values f'or coho fry and coho smolt are slightly higher than reported by Finlayson and Verrue (1980, 1982) for juvenile chinook salmon in a solution with copper and zinc present in a ratio of 1:3. 'Their average 96-hour LC50 results with juvenile chinook salmon (39-68mm) are 33 ug/l copper and 102 ug/l zinc, whereas with 90-day swim-up fry the results were 48 ug/l copper and 144 ug/l zinc. Our steelhead smolt results show good agreement with those reported by Finlayson and Aschuckian (1979) for juvenile steelhead trout.
Long-term LC50 Tests (Total Metal)
Table 6 summarizes the LC50s for each project with a duration of nine days or longer in duration. Table 7 presents the LC50s at approximately 9 days, the time period with the largest long-term data base. Generally, the 9-day LC50s were less variable and i
lower than the 96-hour LC50s. The 9-day LC50s for total copper with coho and steelhead ranged from 61-84 ug/l and 55-87 ug/l respectively . These LC50 values are higher than many of the values previously reportea for salmonids in water with comparable hardness and pH (Miller and McKay, 1980; Chapman, 1978a; Lloyd, 1961; Grande, 1966). A possible explanation for the difference is that the referenced studies were performed in " artificial" or filtered water and lacked many or all of the anions and organic compounds known to complex metals. Our results are lower than f those - found by Holland et al. (1960) and Fogels and Sprague
) (1977) which were conducted in harder, more alkaline water.
Fry and smolt life stages of coho salmon show similar response to copper. This agrees with the work of Chapman (1978a) who found l little variation in LC50s among life stages with chinook and steelhead exposed to copper.
L i At the concentrations . tested, mortality resulting from zine toxicity was virtually complete prior to the ninth day, '
regardless of the length of the experiment. No coho died between the ninth and the sixtieth day and the few steelhead which did die after the ninth day were exposed to zine concentrations above 220 ug/1. The long-term zine LC50s for coho parr and/or smolt ranged from 465-849 ug/l while the two values for steelhead smolt were 89 and 213 ug/1. Our results are in good agreement with Chapman's (1978a) 8-day LC50s of 93, 120, and 278 ug/l for steelhead swim-up fry, parr and smolt respectively. However, our long-term LC50s for coho are higher than the 8-day LC50s of 395
, and 364 ug/l reported by Chapman (1978a) for chinook salmon parr i and smolt respectively. In the same report, Chapman noted that sockeye salmon (Oncorhynchus nerka) were relatively 1.6 times more tolerant to zine than chinook. It appears that coho may be intermediate between chinook _ and sockeye in their tolerance to zinc. -
Five 9-day tests'were successfully completed which allow an assessment of the manner in which the toxic effects of copper and zinc interact (Projects 4, 6, 6A, and 7 with coho and Project 7 with steelhead). Of particular interest was Project 7 in which both species of fish had higher copper LC50s in the presence of zine than with copper alone (Table 7). While the difference was nct statistically significant for coho, it was for steelhead, and a clear indication of antagonistic effect is present. In the other three cases, aditive effects are indicated.
By comparing all of the 96-hour and 9-day two-metal results, it appears that the toxic interaction effect (additive, synergistic, or antagonistic) is a function of time, and possibly, the relative concentration of the two metals. when zinc is present in high concentrations relative to copper, the interaction effect may be synergistic. As the relative proportion of copper increases (total concentration of zinc decreases), the effect becomes additive, and as time increases so that the concentrations decrease, there is an indication of antagonistic interaction.
Subacute Tests (Total Metal)
Sixty-day mortality and growth studies were conducted to assess subacute effects of copper and zine individually and in combination. The results of these tests are summarized in Tables 8 through 14 with the presence of weights which differ significantly from all controls as well as non-random mortalities outlined. The data on fish condition in Project 8 are presented in Tables 15 and 16, and the "in-stream, no effect level" (ISNEL; based on mortality, growth, or condition) by metal, species, project, and indicator is presented in Table 17.
Length proved to be an insensitive measurement and did not provide any meaningful information helpful in reaching 12
DRAFT conclusions. Of the two a posteriori tests, Duncan's Mulitple Range Test was the mdst sensitive. Regression analysis across the entire sixty day tect did not provide as much sensitivity as did the a posteriori tests performed one day at a time. In no caso did a difference between slopes result in a conclusion of a lower effect level than that found by Duncan's test.
In six of the nine tests (Table 17), mortality proved to be a more sensitive indicator of the ISNEL than either growth or condition. In the only single metal test where growth was a more sensitive indicator (coho with copper in Project 5), there was virtually no dif ference between between the value for growth (22 ug/1) and that for mortality (24 ug/1). In both of the two metal tests, however (Project 8), both growth and condition were more sensitive indicators than nortality. A seasonal effect on toxicity appears to be present as the ISNEL was significantly higher during Project 8 (fall) than either Project 5 or 6 (spring and summer).
The gross anatomical tissue condition and parasite count (Tables 15 and 16) did not prove to be a particularly sensitive method for identifying sublethal effects of either copper or zinc.
There was clearly no trend present for the steelhead; however, it is possible that sublethal effects were observed in the three most toxic tanks on coho. The sum of the average tissue
~
conditions in these three tanks is less than that of all of the other toxicant tanks and three of the control tanks. The lowest total was present in a control tank. Previous studies on anatomical changes related to copper concentration are limited.
Baker (1979) found a 28-day exposure period to 180 ug/l copper altered the gill ultrastructure of winter flounder. Whereas liver and interrenal tissues were apparently unaffected at 560 ug/l in those fish surviving the 28-day exposure.
l The effect of copper or copper and zinc in combination on the growth of coho is in good agreement with similar work performed by Finlayson and Verrue (1980). Our findings for steelhead indicate a slightly less toxic effect of copper than some other studies. Mckim et al. (1978) and Waiwood (1977) reported chronic effects of copper on growth at 31.7 and 15 ug/l respectively.
Sinley et al. (1974) reported a maximum acceptable toxicant r concentration (MATC), based on mortality for rainbow trout, not exposed to zinc, in the egg stage of between 36 and 71 ug/1; this agrees well~ with our data. The ISNEL for zinc and steelhead growth (>l79 ug/1) is compatible with the no effect values of 140-260 ug/l which Sinley et al. (1974) observed with rainbow trout. Our no effect level for zine on coho growth and mortality
(>326 and 220 ug/1, Table 17) are slightly lower than reported by Chapman (1975, 1978b) for chinook and sockeye salmon.
If regulatory and resource agencies must focus on one number which is safe for the most sensitive species on a year round basis, our tests suggest 18 ug/l total copper (coho, Project 6, mortality) and 59 ug/l total zine (steelhead, Project 5, mortality). While two metal tests were not conducted in either 13
DRAFT Project 5 or Project 67 The results from Project 8 indicate that these low levels of zine have no effect on the ISNEL of copper.
Metal Speciation and Water Quality As noted earlier, one of the objectives was to understand the effect of water quality and metal species on the toxicity of copper and zinc. Water quality variation, either seasonally or at different locations, is believed to play a major role in establishing the toxicity of metals. Additionally, many individuals now speculate that it is a subcomponent of the total metal (e.g. dissolved, ionic, or labile) which more nearly indicates how toxic the solution is to fish (Chapman and McCrady, 1977); Mulvihill and Kruger, 1976; Pagenkopf, 1983). To date, there has been very little hard evidence to support this theory because of the difficulty in measuring metal species.
Insufficient data was collected to perform an in depth analysis of the effect of water quality parameters on the toxicity of zinc species. Nevertheless, we did observe that both dissolved and labile zine were always .present and represented between 74 and 92% of the total zinc. Because labile zinc was always present, there was no zinc binding capacity in the Chehalis River or in any of the tanks. In general, we conclude that labile and dissolved zinc differ only by measurement error. Furthermore, because of the narrow band in which this proportion fell over nearly a full annual cycle, and because of the difficulty in making the measurements relative to measuring the concentration of total zinc, it appears that the benefit obtained from making the measurements is not justified by the effort. Certainly, it appears that regulation of zinc concentration by total metal will be as effective as regulating by either dissolved or labile components.
As with the two forms of zinc, dissolved copper was present as a nearly constant proportion of the total. Except for one case (Project 4), all dissolved copper averages were between 74 and 81% of the total copper. Because the lowest pH of the entire study (Table 3) was present during Project 4, we consider the value of 54% dissolved copper suspect; typically, the percentage of dissolved copper increases with a decrease in pH. As with zinc, , we consider measurements of dissolved copper to be only marginally useful.
Unlike the other metal species discussed above, this study indicates that labile copper may be a better indicator of toxicity than the total metal for long-term effects. Data on metal species are summarized for the acute tests in Tables 18 and
- 19. Of particular interest is the difference between the LC50s ,
for coho of projects 6 or 6S and 6A. The first two projects were run simultaneously utilizing different life stages and significantly different concentration ranges while Project 6A was run immediately after Project 6 acute studies. The range of LC50s for coho was 62-88 ug/l at 96 hours0.00111 days <br />0.0267 hours <br />1.587302e-4 weeks <br />3.6528e-5 months <br /> and 61-84 uq/1 at 9 days when based on total copper, but narrowed to 42-46 ug/l at 96
DRAFT nours and 38-46 ug/l at 9 days when based on labile copper. The water quality parameters were almort identical except that during Project 6A we observed the higaest concentration of ambient cations encountered during the study. These observations are similar in nature to those of Andrew (1976) working with fathead - r minnows (Pimephales promelas) in two different waters. The total copper LC50 ranged from 200-800 ug/l while the LC50 based on cupric ion was in the narrow range of 0.070 to 0.055 uM/1.
an effort to more fully understand which natural conditions In might be contributing most to the variability of the LC50s, correlation analyses were performed on the concentration of the copper species and the average value of water cuality parameters collected during the 96 hour0.00111 days <br />0.0267 hours <br />1.587302e-4 weeks <br />3.6528e-5 months <br /> or 9 day period (Table 20).
Correlation analysis was not performed with steelhead at 96 hours0.00111 days <br />0.0267 hours <br />1.587302e-4 weeks <br />3.6528e-5 months <br />
~
because there were only two LC50 estimates. Additionally, the _
analysis with steelhead is very weak at 9 days because there are only three data points. The correlation matrix for the water quality parameter averages used in the metals analysis is presented in Table C-3.
Immediately apparent in Table 20 is that the only statistically -
significant correlations between an LC50 and water quality parameters occur under the 9-day labile copper column for coho; temperature, hardness, conductivity, and alkalinity are all significantly correlated with the labile metal LC50. The
. uniformly low correlation coefficients present between the coho i LC50s and the water quality parameters at 96 hours are particularly interestina in view of the ' generally held belief that water quality factors play a major role in determining LC50s. Perhaps there was simply not enough variation present in -
the Chehalis River, relative to the differences in water utilized by different researchers, for water cuality to have a measurable _
These results are the opposite of offect at 96 hours.
i conclusions presented by the EPA (1980a,b) in which hardness was (
judged more important in short term than long term tests. -7 The correlation analysis for steelhead are judged to be -
inconclusive. Of the three LC50 pairs, two (Projects 4 and 5) '
had the same total copper LC50 and differed by only 1 ug/l in -
labile metal. The high, but not statistically significant, due to sample size, correlations with water cuality parameters are probably real and are generally for the same parameters which were significant for coho.
Having identified correlations with water quality parameters, we performed both stepwise and simple two variable linear regressions of labile copper LC50 vs average water quality ,
parameters. The regressions of LC50 against temperature are ;
presented with the plots in Figures 9 to 11. For the coho at 9 days, we could account for 94.6% of the' observed variation in the LC50s by including temperature and iron in the regression and $50 99.7% by adding COD. In spite of the fact that the regressions kJC had statistically significant F and t ratios, we did not include b[p either iron or COD in the equation we report because they were 'f r, )
M_
15 (A
DRAFT not significantly correlated with the LC50, and there were not many data points. As ,would be expected from the lack of a significant correlation, there was not a significant regression between the 96-hour LC50 for coho and labile copper against temperature (Figure 9) although there would be if only one of the five data points (Project 7) was removed.
Because the primary objective of this study was to estimate the ISNEL of copper and zinc in ambient Chehalis River water, no effort was made to control the experiments for any water quality parameters. As a result, we frequently experienced wide variation in water quality parameters during the subacute tests and are, therefore, reluctant to correlate those parameters with seasonal differences of the ISNEL. Rather, we have presented the ISNEL, as indicated by either growth or mortality, for each species by project and metal species at approximately 10, 30, and 60 days (Table 21). We have also presented, the lowest concentrations of copper species at the above times in which an effect was observed (Table 22).
From Figure 8 it can be seen that the copper binding capacity of the Chehalis River varied seasonally with a peak of approximately 22 ug/l during the early fall. During Project 5, the binding capacity was initially very low (~ 7 ug/1) and then increased rather steadily. The binding capacity during Project 6 was fairly constant (~ 16 ug/1) while the binding capacity during
. Project 8 was initially very high (21-22 ug/1), remained uniformly high for approximately 30 days, and then fell off rapidly during the second half of the project to ~ 8-9 ug/l. By comparison, the temperature range was virtually identical during Projects 5 and 8 (7.1-13.7 vs. 13.8-4.7 respectively) but increased during Project 5 and decreased during Project 8. The temperature range during Project 6 was 14-19.6C and generally increased (Figure 2).
Mortality patterns during the tnree subacute tests were different at similar concentrations of total copper. For example, comparing mortalities at a concentration of 51-53 ug/l (Tables 8, 12, and 13) we can see that in Project 5 mortalities occurred early in the te st and then ceased; in Project 6 mortalities continued throughout, while in Project 8 there was only one mortality during the entire test and we judge it to be random in nature. It is also interesting that we never observed any long-te rm effects unless labile copper was present even though many tanks contained no labile copper. While acknowledging that others may speculate about alternative mechanisms to account for the above observations, we theorize the following:
During the initial days of Project 5 when the capacity of the Chehalis River to bind copper was low, copper began affecting the fish. As the binding ' capacity increased, toxicity of total copper was lowered. A combination of reduced toxicity and, probably, acclimation resulted in an end of mortality.
Temperatures were never high enough to play a significant role.
16
During Project 6, temperature was a very significant variable as it approached what is generally considered to be the incipient lethal limit of 21 degrees C for most salmonids (USEPA et al.,
1971). As previously discussed, our acute tests indicate a relationship between copper toxicity and temperature. By the start, of Project 8, however, temperature had declined sufficiently so that it was not a major factor while binding capacity of the Chehalis River reached its annual peak; as a result, copper was significantly less toxic than it had been i earlier in the year. During the first thirty days of Project 8, and later as binding capacity tapered off, the fish became acclimated to the copper and were not as subject to toxic effects at concentrations which were lethal at other times of the year.
In conclusion, we believe that labile copper may be preferable to total copper as an indicator of toxicity. Labile copper may also possess the additional advantage of being an integrator of water quality factors and the data may, therefore, be more directly applicable to different water systems.
I r
17
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Chakoumakos, C., R. C. Russo, and R. V. Thurston. 1979. Toxicity of copper to cutthroat trout (Salmo clarki) under different conditions of alkalinity, pH and hardness. Environ. Sci. Technol. 13 (2): 213-219.
Chapman , G. A. 1975. Toxicity of copper, cadmium and zine to pacific northwest salmon. Interim Report Task 002 ROAP 10 CAR. National Environmental Research Center. United States Environmental Protection Agency. 27 pp.
Chapman, G. A. 1978a. Toxicities of cadmium, copper, and zine to four stages of chinook salmon and steelhead. Trans. Am. Fish. Soc.
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Chapman, G. A. 1978b. Effects of continuous zine exposure on sockeye salmon during adult-to-smolt freshwater residency. Trans. Am. Fish.
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Chapman, G. A. and J. K. McCrady. 1977. Copper toxicity: a question of form. In recent advances in fish toxicology: a symposium. Edited by R. A. Tubb. U.S. EPA Rep. No. EPA 660/3-77/085. 132-151.
Chapman, G. A. and D. G. Stevens. 1978. Acutely toxic levels of cadmium, copper and zine to adult male coho salmon and steelhead.
Trans. Am. Fish. Soc. 107:837-840. -
Chau, Y. K., R. Gachter, and K. Lumm-Shue-Chan. 1974. Determination of the apparent complexing capaity of lakewater. J. Fish. Res. Bd. Can. 31:(9) 1515-1519.
Finlayson, B. J. and S. H. Ashuckian. 1979. Safe zine and copper levels from the Spring Creek drainage for steelhead trout 'in the upper Sacramento River, California. Calif. Fish and Game 65:80-99.
Finlayson, B. J. and K. M. Verrue. 1980. Estimated safe zine and copper levels for chinook salmon, Oncorhynchus tschawvtscha, in the upper Sacramento River, California. Calif. Fish and Game 66:68-82.
Finlayson, B. J. and K. M. Verrue. 1982. Toxicities of copper, zinc, and cadmium mixtures to juvenile chinook salmon. Trans. Am. Fish. Soc.
111:645-650.
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Fogels, A. and J. B. Sprague.
DHAH 1977. Comparative short-term tolerance of zebrafish, flagfish and rainbow trout to five poisons including potential reference toxicants. Water Res. 11:811-817.
Garton, R. R. 1980. A simple continuous-flow toxicant delivery system.
Water Res. 14:227-230.
Goetti, J. P. Jr., J. R. Sinley, and P. H. Davies. 1972. Water pollution studies. Job Prog. Rept., Fed. Aid Proj. F-33-R-7, Colo. Div.
Wildlife, Denver, Colo.
Grande, M. 1966. Effect of copper and zine on salmonid fishes. Advan.
Water Pollut. Res. 1: 97. 3rd Conf. Munich.
Greter, F. L., J. Buffle, and W. Haerdi. 1979. Voltammetric study of humic substances 1: Study of the factors influencing the measurement of their complexing properties with lead. J. Electroanal. Chem. 10: 211-229.
Hamilton, M. A., R. C. Russo, and R. V. Thurston. 1977. Trimmed Spearman-Karber method for estimating median lethal concentrations in toxicity bioassays. Environ. Sci. Technol. 11:714-719.
Hodson, P. V., U. Borgmann, and H. Shear. 1979. Toxicity of copper to aquatic biota. In J. O. Nriagu, ed. Copper in the environment part II:
Health effects. New York, pp. 307-372.
Holcombe, G. W. and R. W. Andrew. 1978. The acute toxicity of zine to rainbow and brook trout comparisons in hard and soft watet. United States Environmental Protection Agency Ecological Research Series, EPA-60013-78-094.
Holland, G. A., J. E. Lasater, E. D. Newman and W. E. Eldridge, Toxic Effects of Organic Pollutants on Young Salmon and Trout, Research Bulletin No. 5, State of Washington, Department of Fisheries, Olympia, Washington, 1960, 264 pp.
Howarth, R. S. and J. B. Sprague. 1978. Copper lethality to rainbow trout in waters of various hardness and pH. Water Res. 12:455-462.
Lloyd, R. 1961. The toxicity of mixtures of zine and copper sulphates to rainbow trout (Salmo ,gairdneri R.). Ann. Appl. Biol. 49: 535.
Lorz, H. W. and B. P. McPherson. 1976. Effects of copper and zine in fresh water on the adaptation to sea water and Atpase activity, and the effects of copper on migratory disposition of coho salmon Oncorhynchus kis6tch. J. Fish Res. Board Can. 33:2023-2030.
Marking, L. L. and V. K. Dawson. 1975. Investigation in fish control. 67.
methods for assessment of toxicity or efficacy of mixtures of chemicals. U.S. Dept. of Interior. Washington, D.C. 7pp.
McKim, J. M., J. G. Eaton, and G. W. Holcombe. 1978. Metal toxicity tb embryos and larvae of eight species of freshwater fish - 11: copper.
Bull. Environ. Contam. Toxicol. 19: 608-616.
19
Miller, T. G. and W. C. MacKay.
T NAFT 1980. The effects of hardness, alkalinity and pH of test water on the toxicity of copper to rainbow trout (Salmo cairdneri). Water Res. 14: 129-133.
l Mulvihill, E. and R. Kruger. 1976. Analytical study of copper and its f chemical forms in a closed cycle cooling system as related to the l
aquatic environment. Contributed paper, pacific northwest pollution control assn., Seattle. Oct. 28, 1976.
I Nehring, R. B. and J. P. Goetti, Jr. 1974. Acute toxicity of a zinc polluteo stream to four species of salmonids. Bull. Environ. Contam.
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Samuelson, D. F. 1976. Water quality: wester fish toxicology station and western Oregon rivers. USEPA Report No. EPA-600/3-76-077. Corvallis, OR. 64pp.
Saar, R. A. and J. H. Weber. 1982. Fulvic acid: modifier of metal ion chemistry. Env. Sci. Tech. 16:510-517.
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Sinley, J. R., J. P. Goetti, Jr., P. H. Davies. 1974. The effects of zine on rainbow trout (Salmo cairdneri) in hard and soft water.
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20
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m 9
21
TABLE 1 REPRESENTATIVE
- WEIGHT / LENGTH DATA FOR C0HO SALMON AND STEELHEAD TROUT PROJECT NUMBER Species 3 4 5 6,6S 7 8 Coho salmon 20 20 30 30/20(b) 20 30 Number (n) weight, gm 1 mean (R) 15.1 14.7 16.9 1.4/31.0 6.2 7.7 Std. Dev. (S.D.) 4.4 2.9 3.9 0.3/ 5.3. 1.5 1.1
~ length, cm i 11.3 11.3 11.5 5.2/14.6 8.5 8.9 S.D. 1.0 0.7 0.9 0.3/ 0.9 0.6 0.4 ,
l Steelhead trout 20 20 15 15 20 20 n
weight i 13.5 25.0 25.2 60.7 20.8 24.8 S.D. 4.1 6.0 5.5 20.1 4.2 6.1 length i 11.2 13.8 13.6 18.4 12.5 13.1 1.3 1.1 1.1 2.3 0.8 1.1 S.D.
(a) Data from one tank measured'at the beginning of Project 5, 6S, and 8 and from one control tank at the end of other (acute) projects.
(b) Fry /smolt
TABLE 2 WATER QUALITY SAMPLING PARAMETERS, FREQUENCIES AND LOCATIONS Acute Subacute EPA Method -
Parameter Reference i Frequency Location Frequency Location Total copper and 220.2 Beginning All 48 aquaria, Beginning of Same as acute zinc 289.2 at least once river & head box test, weekly during test thereafter pH, dissolved oxy- 150.1, 360.1, Daily 6 aquaria / diluter Same as acute Same as acute gen, temperature 170.1, 120.1 Alkalinity, 310.1, Weekly I control Same as acute Same as acute hardness 130.1 aquaria Dissolved copper - Beginning of I control, high Weekly Same as acute and/or zinc test and medium, low at 96-hr toxicant tank /
diluter Electroactive - Once per test "
Weekly' Same as acute copper and/or zinc Total complexing - River sample Weekly Same as acute capacity Chemical oxygen 410.2, Beginning of Head box and Weekly Same as acute demand, total 160.2, test and River suspended solids 325.3 every 30 d chloride Sulfate 375.4 Honthly River and 1 Same as acute Same as acute control aquaria l Ammonia 350.3 Once per test Control and Weekly Same as acute I high toxicant aquaria Cr, N1, Cd, Pb, See text Honthly River and 1 Same as acute Same as acute Ca Hg, K NA, Fe control aquarta
CHEHALIS RIVER WATER QUALITY CHARACTERISTICS DURING THE WNP-3 BI0 ASSAYS: 1982
' PROJECT NUMBER 3 4 4* 5 6S 6 6A 7 8 Temperature 9 5 6 62 62 5 4 7 67 number = (n) mean,(x)0 c 4.4 6.0 6.7 9.5 16.7 13.3 15.7 15.3 8.7 Std. Dev. (S.D.) 0.9 0.7 0.5 2.0 1.5 5.4 0.9 0.3 2.6 ,
Range (R) 3.2-6.1 5.3-7.0 6.0-7.2 6.6-13.2 13.6-19.2 13.8-16.2 14.8-16.8 15.1-16.0 3.9-13.3 62 62 5 4 7 67 Dissolved oxygen (n) 9 5 6 i, mg/l 12.1 11.1 11.1 10.8 9.7 10.3 10.7 8.9 10.6 S.D. 0.6 0.3 0.2 0.4 0.8 0.5 1.0 0.2 0.7 R. 11.1-12.7 10.6-11.4 10.8-11.4 9.6-12.1 8.5-12.2 9.7-10.9 9.8-12.2 8.7-9.3 9.3-12.4 4 7 66 pH (n) 1 4 6 56 61 5 Median, S.U. 6.9 6.8 6.9 7.1 7.3 7.3 7.5 7.2 7.0 S.D. -
0.2 0.1 0.1 0.2 0.0 0.3 0.1 0.2 R. -
6.6-6.9 6.7-7.0 7.0-7.3 6.9-7.9 7.3-7.4 7.2-7.8 7.0-7.3 6.6-7.4 Conductivity at 25 C 8 5 5 27 2 0 0 3 4 n
70 62 55 71 87 95 70 i umhos/cm 4 3 4 4 S.D. 8 7 1 65-90 57-73 52-61 65-79 86-88 92-99 66-76 R.
- Test performed one week later.
l DRAFT TABLE 4 i
SUMARY OF METALS DATA: WNP-3 BI0 ASSAY Parameters l'
ug/l ug/l ug/l ug/l mg/l mg/l mg/l mg/l mg/l Cr Ni Cd Pb Ca Mg, K Na Fe l Mean
- 1.3 1.1 0.3 1.5 7.08 2.38 0.653 5.117 0.99 Standard 0.6 0.3 0.04 1.0 1.17 0.37 0.154 0.853 0.84 Deviation -
Minimum 1.0 1.0 0.2 0.2 4.86 1.81 0.469 3.941 0.21 Maximum 3.0 2.0 0.4 4.4 9.24 3.23 1.091 7.116 3.38
- Number of values = 24 for each parameter
TABLE 5 96-H00R LC50s PROJECT 3 4 5 6 6A 7 Coho Copper 81 85 $90 61/63 I) 86/90 IO) 103 Zinc 2022 1900 II) > 612 Copper (Cu/Zn) 36 55 77/79 84 Zinc (CuiZn) 754 160 166/171 267 Steelhead Copper 93 II) 94(2) >89 90 Zinc 688 406 > 612 Copper (Cu/Zn) 46(3) 45 69 Zinc (Cu/Zn) 389(3) 128 214 (1) 15% Trim (2) 21% Trim (3) Toxicant flow lost at approximately 89 hours0.00103 days <br />0.0247 hours <br />1.471561e-4 weeks <br />3.38645e-5 months <br />, diluted to 25% strength at 96 hours0.00111 days <br />0.0267 hours <br />1.587302e-4 weeks <br />3.6528e-5 months <br />. 16% Trim.
(4) LC50s not calculable. Coho copper visually estimated on mortality data, other data sets lower bound based on highest concentration tested.
(5) Fry /smolt (6) Uncalculable for smolt. Value of 90 is visually estimated from data.
TABLE 6 LONG TERM LC50s 3 4 5 6 6A 6S 7 8(8) llours 248 696 1449 242 258 696 1440 215 1440 Coho Copper 70(240) (b) 65 >51 60/61(c) 77/84 51(d) 39 69 >52 Zinc 575 849(210) 465(*) >326-Copper (Cu/Zn) 31(209) 47/ 72/76 72 >52 Zine (Cu/Zn) 637(209) 137/ 159/166 224 >155 Steelhead Copper 84(240)ff) 70(E) 51 55 >52 Zinc 213 89(256) 120 S120 Copper (Cu/Zn) / 42 67 >52 Zinc (Cu/Zn) /122 206 >155 (8) Mortality in all tanks was very low; the highest concentration tested is listed.
(b)ILL (flours)
(c) Fry /Smolt (d)25% Trim (e)30% Trim (f)21% Trim (El l5*', Trim I
TABLE 7 NINE-DAY LC50s s PROJECT 3 4 5 6 65 6A 7 Hours 226 209 216 220 220 209 215 Coho Copper 72 74(2) 61/61 62 82/84(3) 69 Zinc 754 849 465(5)
Copper (Cu/Zn) 31 47/ 72/77 72 Zinc (Cu/Zn) 637 137/ 159/177 224 Steelhead Copper 87(1) 87(3) 55 Zinc 213 89 119 I4)
Copper (Cu/Zn) /42 67 Zinc (Cu/Zn) /122 206 (1) 21% Trim (2) 15% Trim (3) Estimated by interpolation, mortality too low for spearman-karber (4) 20% Trim f55 30% Trim
[6D Fry /Smolt
~
PROJECT 5 WEIGHT / LENGTH
SUMMARY
DRAFT SPECIES: C0HO ,
METAL 1 COPPER DATE1 MARCH 15 MARCH 25 APRIL 5 APRIL 15 APRIL 26 MAY 6 MAY 17 DAYS: -2 8 19 29 40 50 61 AVERAGE LENGTH WEIGHT E5E5iHWEIGHT E505iE~U515Ai E555is U5555i E5A5iH WEIGHT LENGTH WEIGHT i.ENG PPB CONTROL AVERAGE 11.50 16.81 11.65 16.55 11.75 17.33 11.90 18.09 12.16 19.13 12.48 20.50 12.88 22.81 STD DEV - 0.75 3.32 0.73 3.15 0.72 3.15 0.69 3.16 0.65 3.12 0.60 3.21 0.63 3.55 NUMBER 30 30 30 29 29 29 29 CONTROL AVERAGE 11.47 16.87 11.58 17.09 11.85 18.16 12.03 19.11 12.21 19.87 12.48 21.26 12.86 23.09 STD DEV 0.86 3.88 0.86 3.68 0.81 3.69 0.81 3.85 0.81 3.97 0.82 4.24 0.84 4.61 NUMBER 30 30 30 30 30 30 30 CONTRGL AVERAGE 11.72 17.34 11.79 17.58 12.02 18.02 12.15 18.77 12.32 19.72 12.57 20.94 12.87 22.88 STD DEV 0.69 2.70 0.69 2.83 0.70 2.96 0.68 3.03 0.69 3.30 0.72 3.60 0.77 4.07 NUMEER 30 30 30 30 30 30 30 CONTROL AVERAGE 11.68 17.18 11.77 17.83 12.01 18.78 12.21 19.58 12.41 20.35 12.69 22.10 13.05 24.03 STD DEV 0.91 3.65 0.65 3.65 0.87 3.85 0.85 3.99 0.83 4.08 0.83 4.40 0.84 4.72 NUMBER 30 30 30 30 30 30 30 7 AVERAGE 11.77 17.84 11.83 17.52 12.00 18.35 12.14 18.83 12.25 19.62 12.52 20.84 12.84 22.78 STD DEV 0.78 3.39 0.80 3.28 0.77 3.32 0.78 3.41 0.78 3.62 0.81 3.77 0.80 4.19 HUMBER 30 30 30 30 30 30 30 9 AVERAGE 11.61 17.29 11.73 17.43 11.92 18.36 12.27 19.23 12.37 20.31 12.66 21.59 12.98 23.61 STD DEV 0.63 2.68 0.61 2.61 0.58 2.67 1.12 2.72 0.54 2.76 0.56 2.93 0.59 3.27 NUMBER 30 30 30 30 30 30 30 12 AVERAGE 11.32 16.11 11.52 16.31 11.63 17.16 *1.81 17.90 11.99 18.89 12.28 20.43 12.74 22.71 STD DEV 1.01 4.43 1.00 4.35 0.96 4.33 0.95 4.23 0.89 4.31 0.34 4.49 0.85 4.83 NUMBER 30 30 30 30 30 30 30 16 AVERAGE 11.54 16.95 11.59 16.47 11.69 16.68 11.81 17.50 11.95 18.42 12.19 19.56 12.54 21.53 STD DEV 0.80 3.34 0.79 3.04 0.76 3.01 0.77 3.17 0.79 3.46 0.83 3.85 0.92 4.50 NUMPER 30 30 30 30 30 30 30 22 AVERAGE 11.60 16.66 11.69 15.94 11.70 16.41 11.81 16.79 11.92 17.51 12.19 18.80 12.58 21.30 STD DDJ 0.79 3.33 0.73 3.04 0.72 3.01 0.71 3.21 0.73 3.55 0.78 3.88 0.87 4.48 NUMBER 30' 30 30 30 30 30 30 24 AVERAGE 11.67 17.97 11.77 16.47 11.79 15.99 11.78 15.93 11.78 15.96 11.90 16.45 12.10 17.69 STD DEV 0.76 3.33 0.74 3.10 0.75 3.04 0.76 2.99- 3.76 3.09 0.76 3.49 0.84 4.40 30 30 30 30 30 30 NUMBER
,_ 2 _9 _
43 AVERAGE 11.71 17.98 11.75 16.73 11.74 15.65 11.75 15.55l11.79 12.00 17.23 STD DEV 0.84 3.61 0.84 3.45 0.85 3.34 0.86 3.19 0.86 15.69 3.08 11.89 0.81 16.26 3.22 0.81 3.70 HUMBER 30 30 29 29 j 28 23 28 51 AVERAGE 11.72 17.77 11.68 16.31l11.65 14.77 11.42 14.12 11.51 13.41 11.51 12.61 11.54 12.19 STD DEV 0.74 3.21 0.73 3.12 0.76 2.83 0.75 2.67 0.75 2.62 0.76 2.43 0.78 2.49 NUMBER 30 29 j 26 26 26 26 26 78 AVERAGE 11.40 16.34 11.35 15.41 11.44 14.50 11.29 12.93 STD I(V 1.05 4.01 1.18 4.69 1.34 4.66 HUMBER 30 4.26 l 1.00 19 9 7 TEST DISCONTINUED 89 AVERAGE 11.82 18.01 l 11.82 17.54 12.80 19.99 12.40 24.61 l STD DEV 0.78 3.58 i 0.59 2.44 NUMBER 30 1 5 1 1_ j TEST DISCONTINUED SOLID LlNE OUTLINES CELLS WHICH HAVE WEIGHTS SIGNIFICANTLY LOWER THAN ALL CONTROLS.
DASHED LDIE OUTLINES CELLS WITH NORTALITY GREATER THAN RANDOM MORTALITY.
e 7
F ECT 5 I WEIGHT / LENGTH SurMARY SPECIES: STEELHEAD ,
METF.L: C0FFER DATE: MARCH 15 MARCH 25 APRIL 5 AFRIL 15 APRIL 26 MAY 6 MAY 17 DAYS: -2 8 19 29 40 50 61 AVERAGE [iS5iSTEi55i [555iSTEi55i El5 i5l[id5i E NGTH QEIGHT i.ENGTH WEIG5i LENGTH WEIG5T E555i515555i FP3 CONTROL AVERAGE 13.09 22.64 13.23 24.45 13.70 26.87 14.03 29.76 14.81 34.12 15.36 37.78 15.94 42.02 STD DEV 1.29 6.65 1.42 7.43 1 47 8.14 1.56 9.47 1.53 10.16 1.64 11.49 1 64 12.98 NUMBER 15 15 15 15 14 14 14 CONTROL AVERAGE 13.62 25.10 13.87 27.25 14.45 31.10 15.01 34.27 15.63 38.81 16.29 43.30 16.89'49.28 STD DEV 1.08 5.48 1.09 5.99 1.10 6.79 1.10 7.16 1.14 8.33 1.19 9.40 1 29 11.08 HUMBER 15 15 15 15 15 15 15 CGNTRGL AVERAGE 13.39 23.70 13.63 26.61 14.11 28.95 14.56 32.50 15.24 36.60 15.79 40.67 16.24 44.59 STD DEV 1.44 7.31 1.47 8.64 1.50 9.07 1.49 9.85 1.59 11.21 1.74 13.00 1.90 15.33 NUMBER 15 15 15 15 15 15 15 CONTROL AVERAGE 12.93 22.10 13.20 24.19 13.64 26.14 14.10 29.00 14.63 32.47 15.14 36.31 15.59 40.10 STD DEV 1.10 5.66 1.09 6.16 1.11 6.69 1.12 7.23 1.16 7.91 1.27 9.20 1.41 11.15 NUMBER 15 15 15 15 15 14(1) 14 7 AVERAGE 12.91 22.04 13.09 23.64 13.50 26.22 14.01 29.76 14.71 33.54 15.31 37.54 15 73 42.02 STD DEV 1.15 5.30 1.17 5.69 1.15 6 08 1.13 7.07 1.19 7.96 1.30 8.84 1.35 10.35 NUMBER 15 15 15 15 15 15 15 9 AVERAGE 13.49 24.50 13.67 26.24 14.11 28.54 14.51 31.61 15.07 34.35 15.71 38.28 15.99 41.87 STD DEV 1.23 7.18 1.22 7.25 1.14 7.70 1.11 8.08 1.17 9.34 1.24 11.75 1.26 8.64 NUMBER 15 15 15 15 15 15 14 12 AVERAGE 12.74 20.96 12.98 22.93 13.46 25.54 13.97 29.06 14.59 32.92 15.23 37.00 15.66 41.38 STD DEV 0.97 4.71 1.02 5.67 1.08 6.59 1.09 7.43 1.20 8.78 1.26 9.56 1.41 10.95 NUMBER 15 15 15 15 15 15 14 16 AVERAGE 12.92 21.94 13.21 24.04 13.61 26.11 13.99 29.26 14.61 32.52 15.19 36.30 15.76 41.59 STD DEV 0.90 4.10 0.94 4.50 0.92 4.79 0.96 5.57 1.00 6.19 1.16 7.65 1 28 9.54 NUMBER 15 15 15 15 15 14 14 22 . AVERAGE 13.34 23.36 13.61 25.58 14.04 28.29 14.51 31.93 15.16 35.25 15.64 38.95 16 13 44.47 STD DEV 1.18 5.54 1.14 5.99 1.11 6.62 1.14 7.60 1.16 8.00 1.13 S.10 1 25 9.94 NUhBER 15 - 15 15 15 15 15 15 24 AVERAGE 12.71 21.42 12.91 22.83 13.31 25 52 13.71 28.46 14.31 31.86 14.94 36.43 15.39 39.78 STD DEV 1.07 5.17 1.07 5.54 1.08 6.30 1.11 7.02 1.23 8.31 1.31 9.28 1 38 10.13 NUMBER 15 15 15 15 14 14 14 45 AVERAGE 12.89 21.59 b .28 23.99 13.66 26.22 14.08 28.82 14.71 32.25 15.43 36.80 15.87 .h STD DEV 1.04 1.01 5.31 0.99 5.50 0.95 5.78 0.89 6.24 1 04 8.55 l NUMBER 15 4.97 l 1.14135.21 13 13 13 12 11 L_.
51 AVERAGE 12.77 21.11 12.9822.0U13.11 23.20 13.42 25.30 13.93 28.10 14.43 31.35 14.92 35.42 STD DEV 1.16 5.79 1.21 6.14 l 1.03 5 44 1.08 6.21 1.13 6.74 1.32 8.63 1.36 10.45 15 14 11 11 11 11 NUMBER
] 11 78 AVERAGE 13.2223.75(33.22 22.74 13.00 21.32 13.27 22.08 STD DEV 1.44 7.58 1.42 7.25 1.34 6.92 1.50 7.15 NUMBER 15 i 12 9 8 TEST DISCONTINUED 89 AVERAGE 12.7620.82l12.69 20.31 12.75 18.06 12.60 18.08 STD DEV 0.9715 4.20 l 1.22 95.14 1.91 6.35 1.98 6.04 NUMBER , 2 2 TEST DISCONTINUED (1) FISH JUMPED FROM TANK SOLID LINE OUTLINES CELLS UHICH HAVE WEIGHTS SIGNIFICANTLY LOUER THAN ALL CCHTROLS.
11 ASHED LINE OUTLINES CELLS 'JITH MORTALITY GREATER THAN RANDCM MORTALITY.
l
. TABLE 10 PROJECT 5 %
h Y lj j !
WEIGHT / LENGTH
SUMMARY
SPECIES 1 CCHO ,
METAL' ZINC DATE: MARCH 15 MARCH 25 APRIL 5 AFRIL 15 AFRIL 27 MAY 7 MAY 18 DAYS 1 -2 - 8 19 29 40 50 61 AVERAGE LENGTH WEIGHI L5E5555 U51555 LENGTH WEIGHT LENGTH WEIGHT [ENGTHWEIGH LENGTH UE PFB CONTROL AVERAGE- 11.50 16.81 11.65 16.55 11.75 17.33 11.89 18.09 12.16 19.13 12.48 20.50 12.88 22.81 STD DEV 0.75 3.32 0.73 3.15 0.72 3.15 0.68 3.11 0.65 3.12 0.60 3.21 0.63 3.55 NUMBER 30 30 30 30 30 29 29 CONTROL AVERAGE 11.47 16.87 11.58 17.09 11.85 18.16 12.03 19.11 12.21 19.87 12.48 21.25 12.86' 23.09 STD DEV 0.86 3.88 0.86 3.68 0.81 3.69 0.81 3.85 0.81 3.97 0.82 4.24 0.84 4 61 NUMBER 30 30 30 30 30 30 30 CONTROL AVERAGE 11.72 17.34 11.79 17.58 12.02 18.02 12.15 18.77 12.32 19.72 12.57 20.94 12.87 22.88 STD DEV 0.69 2.70 0.69 2.83 0.70 2.96 0.68 3.03 0.69 3.30 0.72 3.60 0.77 4.07 HUMEER 30 30 30 30 30 30 30 CONTRGL AVERAGE 11.68 17.18 11.77 17.83 12.01 18.78 12.21 19.58 12.41 20.35 12.69 22.10 13.05 24.03 STD DEV 0.91 3.65 0.85 3.65 0.87 3.85 0.85 3.99 0.83 4.08 0.83 4.40 0.84 4.72 NUMBER 30 30 30 30 30 30 30 28 AVERAGE 11.91 18.34 12.04 18.71 12.22 19.42 12.31 19.88 12.52 20.60 12.80 22.10 13.22 24.43 STD IEV 0.74 3.42 0.73 3.38 0.70 3.31 0.69 3.25 0.64 3.36 0.62 3.60 0.62 4.03 NUMBER 30 30 30 30 30 30 30 40 AVERAGE 11.87 18.65 11.95 18.42 12.10 19.23 12.25 19.67 12.43 20.50 12.72 22.20 13.07 24.67 STD DEV 0.71 4.27 0.71 3.23 0.70 3.46 0.73 3.54 0.75 3.95 0.80 4.43 0.82 5.69 NUMBER 31 31 31- 31 31 31 31 39 AVERAGE 11.70 17.68 11.74 17.52 11.91 18.05 12.01 18.42 12.17 19.24 12.40 20.62 12.78 22.65 STD DEV 0.92 4.04 0.94 4.11 0.96 4.19 0.98 4.03 0.94 4.28 0.93 4.45 0.91 4.80 NUMBER 30 30 30 30 30 30 30 66 AVERAGE 11.62 17.37 11.75 17.71 11.96 18.63 12.07 19.11 12.30 20.19 12.55 21 62 12.90 23.62 STD DEV 0.97 4.23 0.98 4.23 0.91 4.39 0.98 4.44 0.93 4.61 0.94 4.75 0.93 5.14 NUMBER 30 30 30 30 30 30 30 93 AVERAGE 11.74 17.86 11.83 18.30 12.01 18.85 12.18 19.90 12.40 21.09 12.74 22.87 12.11 25.62 STD DEV 0 64 3.05 0.67 3.15 0.65 3.06 0.65 3.16 0.65 3.55 0.67 3.80 0.66 4.46 NUMBER 30 30 30 30 30 30 30 119 AVERAGE 11.78 17.91 11.89 18.25 12.06 18.91 12.25 19.65 12.45 21.24 12.73 22.29 13.12 24.57 STD DEV 0.98 3.98 0.97 4.16 1.00 4.44 1.03 4.57 1.01 7.00 1.09 5.47 1.12 6.03 NUMBER 30 30 30 30 30 30 30 179 AVERAGE 11.79 17.80 11.85 17.98 12.07 18.93 12.24 20.21 12.53 21.05 12.72 22.43 13.12 24.71 STD DEV 0.77 3.27 0.77 3.37 0.77 3.33 0.74 3.35 0.72 3.61 0.75 3.79 0.78 4.39 NUMEER 30 30 30 30 30 29 29 220 AVERAGE 11.96 18.49 12.00 18.13 12.16 19.07 12.32 19.88 12.52 20.57 12.75 21.90 13.15 24.30 STD DEV 0.85 3.71 0.86 3.78 0.85 3.72 0.85 3.71 0.83 3.88 0.83 4.12 0.81 4.45 NUMBER 30 30 30 30 30 30 30 326 ' AVERAGE 11.85 17.61 11.97 18.70 12.30 20.04 12.67 22.30 13.20 25.14 l STD DEV 11.71 0.59 17.24l11.63 16.73 2.09 0.46 2.05 0.48 2.30 0.46 2.24 0.48 2.42 0.45 2.47 NUMBER 30 2.71l0.47 24 24 24 24 24 24 U
612 12.22 19.39 12.36 20.29 AVERAGE STD DEV 11.73 0.74 17.81l11.96 3.35 0.81 18.44 3.99 0.83 4.09 0.84 3.88 NUMBER 30 8 8 8 TEST DISCONTD1UED 4
SOLTD LINE CUTLINES CELLS WHICH HAKE WEIGHTS SIGNIFICANTLY LOUER THAN ALL CONTROLS.
DASHED LINE OUTLINES CELLS WITH NORTALITY GREATER THAd RAND 06 h0RTALITY.
TABLE 11 PROJECT 5 j WEIGHT / LENGTH
SUMMARY
k j SFECIESI STEELHEAD ,
METAL 1 ZINC DATE1 MARCH 15 MARCH 25 AFRIL 5 AFRIL 15 APRIL 26 MAY 6 MAY 17 DAYS 1 -2 8 19 29 40 - 50 61 AVERAGE [5N5i5'55l65i [5b5i5'55l55i [555}E~55i65i (( 5i5'55l65i [5U5iS~55i55i [5AGT3 55155i [5NGTH UEi557 FFB CONTFOL AVERAGE 13.09 22.64 13.23 24.45 13.70 26.87 14.03 29.76 14.81 34.12 15.36 37.78 15.94 42.02 STD DEV 1.29 6.65 1.42 7.43 1.47 8.14 1 56 9.47 1.53 10.16 1.64 11.49 1.64 12.98 NUPBER 15 15 15 15 14 14 14 CONTROL AVERAGE 13.62 25.13 13.87 27.25 14.45 31.10 15.01 34.27 15.63 38.81 16.29 43.30 16.89' 49 28 STD DEV 1 08 5.48 1.09 5.99 1.10 6.79 1.10 7.16 1.14 8.33 1 19 9.40 1.29 11.08 NUMPER 15 15 15 15 15 15 15 CONTROL AVERAGE 13.39 23.70 13.63 26.61 14.11 28.95 14.56 32.50 15.24 36.60 15.79 40.67 16.24 44.59 STD DEV 1.44 7.31 1.47 8.64 1.50 9.07 1.49 9.85 1.59 11.21 1.74 13.00 1.90 15.38 NUMEER 15 15 15 15 15 15 15 CONTROL AVERAGE 12.93 22.10 13.20 24.19 13.44 26.14 14.10 29.00 14.63 32.47 15.14 36.31 15.59 40.10 STD DEV 1.10 5.64 1.09 6.16 1.11 6.69 1.12 7.23 1.16 7.91 1.27 9.20 1.41 11.15 NUMEER 15 15 15 15 15 14 12 28 AVERAGE 13.60 24.98 13.87 26.92 14.31 29.34 14.75 33.00 15.26 35.96 15.77 39.93 16.22 44.62 STD DEV 0.83 4.43 0.34 4.49 0.86 4.83 0.89 6.19 0.96 6.68 1.04' 7.66 1.08 8.30 NUMBER 15 15 15 15 15 15 15 40 AVERAGE 13.00 22.45 13.35 24.78 13.81 27.36 14.38 30.86 14.39 34.09 15.41 33.20 15.97 42.94 STD LEV 0.85 4.07 0.87 4.69 0.91 5.13 0.96 6.14 1.03 6.88 1.10 8.03 1.17 ' 9.31 NUMBER 15 15 15 15 15 15 15 59 AVERAGE 12.95 21.30 13.10 23.60 13.60 25.83 14.08 29.04 14.76 33.03 15.40 37.55 15.83 42.13 STD DEV 1.20 5.83 1.18 6.55 1.20 6.88 1.20 7.22 1.17 7 77 1.18 8.61 1.22 9.33 NUMBER 15 15 15 15 15 15 15 66 AVERAGE 13.51 24.81 13.60 25.28 I 13.88 27.42 14.32 30.22 14.84 34.01 15.40 38.79 15.6541.5 STD DEV 1.30 7.15 1.27 7.95 l 1.32 8.65 1.28 9.17 1.29 10.24 1.43 12.47 1 38 13.26 NUMEER 15 14 13 13 13 12(1) 11
_J 93 AVERAGE 13.03 22.16 12.74 20.29 13.30 23.46 13.71 26.42 l STD DEV 0.97 4.69 0.91 4.18 0.85 4.23 0.92 4.72 i NUMBER 15' 8 7 7 TEST DISCONTINUED 119 AVERAGE 13.16 23.01 14.21 28.51 14.64 31.23 15.14 34.76 STD DEV 1.26 5.77 0.99 4.75 0.85 4.61 0.78 4.40 l NUMPER 15 7 7 7 TEST DISCONTINUED 179 AVERAGE 13.40 23.53 13.72 25.74 14.16 27.72 14.52 31.04 15.21 34.85 15.91 40.01 16.50 45.15 I STD DEV 1.05 4.37 1.16 5.19 1.06 5.48 1.12 6.19 1.08 6.56 1.04 7.61 1.13 9.06 l NUMEER 15 8 8 8 8 8 9 i 220 AVERAGE 13.18 22.98 12.55 19.75 12.65 20.26 13.00 22.16 P STD DEV 1.07 3.91 0.64 3.22 0.85 3.41 NUMBER 15 5.04l0.96 4 2 2 TEST DISCONTINUED 326 AVERAGE 12.87 14.00 27.71 14.40 29.61 STD DEV 1.13 21.27l12.87 5.23 1 59 22.41 6.66 NUMBER 15 4 1 1 TEST DISCONTINUED l
612 AVERAGE 12.76 20.98 #12.47 20.63 12.25 20.27 13.40 27.68 STD DEV 1.18 5.34 1 1.34 5.18 0.78 4.48 NUMPER 15 4 2 1 TEST DISC 0llTINUED n
(1) FISH JUMFED FROM TANX SOLID LINE OUTLINES CELLS WHICH HAVE VEIGHTS SIGNIFICANTLY LOVER THAN ALL CONTROLS.
DASHED LINE OUTLINES CELLS WITH MCRTALITY OREATER thall RANDOM MORTALITY.
. TABLE 12 PROJECT 6 ,
WEIGHT / LENGTH
SUMMARY
SPECIES:C0HO METAL. COPPER DATE1 MAY 24 JUNE 7 JUNE 18 JUNE 28 JULY 8 JULY 20 JULY 28 DAYS 1 -4 10 21 31 41 53 61 AVERAGE LENGTH WEIGHT LENGTH WEIGHT LENGTH WEIGHT LENGTH WEIGHT LINGTH WEIGHT L505TH WE!597 f.ENGTH chi FFB CGNTROL A'ERAGE 5.17 1.38 1.73 2.01' 5.78 2.30 2.74 3.39 6.71 3.73 STD DEV 0.46 0.39 0.55 0.69 0.62 0.80 0.96 1.15 0.78 1.31 NUMBER 30 30 30 30 30 29(1) 29 CONTROL AVERAGE 5.17 1.36 1.70 2.01 5.82 2.30 2.71 3.26 6.61 3.57 STD DEV 0.33 0.30 0.45 0.55 0.45 0.65 0.79 0.96 0.59 1.14 NUMPER 30 30 30 30 30 30 30 CONTROL AVERAGE 5.10 1.34 1.72 2.05 5.88 2.34 2.72 3.26 6.66 3.63 STD DEV 0.38 0.35 0.51 0.62 0.52 0.70 0.81 0.95 0.61 1.03 NUMBER 30* 30 30 30 30 30 30 CONTROL AIERAGE 5.19 1.42 1.76 2.07 5.83 2.28 2.59 3.09 6.31 3.41 STD DEV 0.36 0.35 0.47 0.66 0.60 0.83 1.00. 1.23 0.78 1.44 NUMBER 30 30 30 30 30 30 30 5 AVERAGE 5.12 1.31 1.63 1.91 5.69 2.17 2.55 3.05 6.48 3.39 STD DEV 0.32 0.27 0.40 0.54 0.55 0.73 0.94 1.17 0.79 1.35 NUMBER 30 30 30 30 30 30 30 5 AVERAGE 5.14 1.35 1.70 2.04 5.86 2.38 2.82 3.41 6.73 3.75 STD DEV 0.41 0.36 0.47 0.59 0.56 0.73 0.91 1.13 0.73 1.29 NUM?ER 30 30 29 29 29 29 29 8 AVERAGE 5 14 1.35 1.72 1.06 5.88 2.33 2.73 3.29 6.70 3.61 STD DEV 0.36 0.32 0.42 0.54 0.48 0.65 0.82 1.02 0.67 1.14 NUMBER 30 30 30 30 30 30 29 8 AVERAGE 5.14 1.34 1.69 1.88 5.89 2.29 2.70 3.29 6.63 3.62 STD DEV 0.33 0.29 0.41 0.60 0.55 0.68 0.84 1.07 0.73 1.26 NUMBER 30 30 32 30 30 30 30 9 AVERAGE 5.12 1.36 1.72 2.57 5.84 2.35 2.75 3.31 6.58 3.58 STD DEV 0.39 0.33 0.48 3.02 0.54 0.73 0.89 1.09 0.72 1.29 NUMBER 30 29 29 29 29 29 29 9 A'KRAGE 5.09 1.31 1.69 1.99 5.82 2.34 2.76 3.30 6.62 3.62 STD DEV 0.34 0.32 0.53 0.73 0.66 0.88 1.10 1.32 0.90 1.48 NUMBER 30 30 30 29 29 29 29 13 AVERAGE 5.11 1.35 1.71 2.04 5.87 2.33 2.77 3.33 6.69 3.69 STD DEV 0.43 0.40 0.59 -
0.73 0.64 0.84 0.97 1.13 0.68 1.20 NUMBER 30 30 30 30 30 30 30 SOLID LINE OUTLINES CELLS WHICH HAVE WEIGHTS SIGNIFICANTLY LOUER THAN ALL CONTROLS.
DASHED LINE OUTLINES CELLS HITH MORTALITY GREATER THAN RANDOM MORTALITY.
TABLE 12 (CONTlNUED)
FROJECT 6 (CONTINUED)
WEIGHT / LENGTH
SUMMARY
SPECIES: CGHO , l METAll C0FFER '
l DATE: MAY 24 JUNE 7 JUNE 18 JUNE 28 JULY 8 JULY 20 JULY 28 DAYS 1 -4 10 21 31 41 53 61 AVERAGE FPB
[E5GTH WEIGHT LEsGTH WElGiii E5EGTHWEIGHT LENGTA~ WEIGHT LENGTH WE 18 AVERAGE 5.17 1.40 1.75 2.06 5.83 2.34 2.80 3.40 6.70 3.7d STD DEV 0.35 0.32 0.40 0.45 0.39 0.52 0.68 0.84 0.56 1.@8 NUMBER 30 30 30 29 30 30 30 18 AVERAGE 5.09 1.34 1.62 1.95 5.78 2.20 2.68 3.74 6.63' 3.5F STD DEV 0.43 0.39 0.50 0.58 0.55 0.69 0.85 1.01 0.70 1.11 HUMPER 30 30 30 30 30 30 30 21 AVERAGE 5.26 1.44 1.70 2.02 h0.57
.98 3.16 2.81 3.41 6.81 3.S0 STD DEV 0.34 0.32 0.48 0.63 4.18 0.78 0.97 0.69 1.10 NUMBER 30 30 30 28 28 28 28 21 AVERAGE 5.10 1.32 1.52 1.85 l 5.79 2.17 2.57 3.28 6.66 3.63 STD DEV 0.34 0.30 0.43 0.62 1 0.62 0.77 0.96 1.14 0.76 1.25 NUMBER 30 30 30 29 29 28 28 l
33 AVERAGE 5.13 1.34 1.25 1.34 5.42 1.63 1.96 2.36 6.02 2.36 STD DEV 0.31 0.29 0.30 0.41 0.39 0.54 0.66 0.87 0.64 0.94 NUMBER 30 30 30 27 26 25(1) 25 33 AVERAGE 5.15 1.37 1.26 1.37 5.38 1.66 2.02 2.41 6.02 2.58 STD DEV 0.31 0.28 0.32 0.50 0.54 0.72 1.05 1.40 0.99 1.59 NUhBER 30 30 30 27 27 27 26 34 AVERAGE 5.12 1.34 1.27 l 1 7 5.51 1.73 2.17 2.65 6.25 2.84 STD DEV 0.35 0.31 0.36 0.56 0.64 0.76 0.91 1.08 0.79 1.2S 30 30 27 25 HUMEER l 30 24 24
( 34 AVERAGE 5.23 1.43 1.26 l 1.25 5.36 1.37 1.57 1.86 5.73 1.90 STD DEV 0.39 0.36 0.36 0.50 0.46 0.65 0.87 1.09 0.78 1.17 j NUMBER 30 30 l 27 25 22 18 18 15 33 AVERAGE 5.22 1.42 l 0.99 5.17 0.94 0.98 l STD DEV 0.28 0.25 0.25 0.25 0.35 0.23 0.23 l NUMBER 30- 20 16 11 4 0 0 33 AVERAGE 5.19 1.41 1.20 1.01 5.13 0.96 0.93 STD DEV 0.34 0.31 0.26 0.25 0.25 0.19 0.11 l NUMPER 30 18 11 4 0 0 l18 65 AVERAGE 5.13 1.33 l 1.11 0.93 4.98 0.81 STD DEV 0.24 0.21 0.18 0.15 0.19 0.14 l HUMBER 30 10 5 0 0 0 l17 65 AVERAGE 5.21 1.43 l 1.06 0.91 5.10 0.88 4.90 0.83 l STD DEV 0.38 0.33 0.21 0.18 0.26 0.21 NUMBER 30 l12 9 3 1 0 0 0 ISH WAS INADVERDENTLY TRANSFERRED FROM THE CONTROL TANK BEFORE WEIGHING TO A 33 PPB LEVEL TREATMENT TANK AFTER THE TREATMENT TANK HAD BEEN WEIGHED.
l SOLID LINE OUTLINES CELLS WHICH HA"E WEIGHTS SIGNIFICANTLY LOWER THAN ALL CONTROLS.
DASHED LINE OUTLINES CELLS WITH MORTALITY GREATER THAN RANDOM MORTALITY. l
TABLE 13 %
I N FF0 JECT 8 'j ft y, WEIGHT / LENGTH SUMMRY
'J d SPECIES: C0HO ,
HETAL. C0FFER/ ZINC DATE: SEPTEMBER 30 OCTOBER 14 OCTOBER 25 NOVEMIER 4 NOVEMBER 16 NOVEMPER 29 DECEMBER 6 DAYS: -6 8 19 29 41 54 61 AVERAGE EENGlHWEIGHT EENGTH WE!5Ai LENGTH utiisi Eis5is DEIGHT EiA5iA TE555i EINGTH U5i5Ei E505is'U555EI FFB CONTROL AVERAGE 8.88 7.72 9.48 11.17 10.22 12.53 13.45 14.27 10.78 14.13 STD DEV 0.43 1.09 1.41 1.69 0.54 1 88 2.21 2.33 0.59 2.37 NUMBER 30 30 30 30 30 30 30 CONTROL AVERAGE 8.92 7.65 9.52 11.38 10.28 12.77 13.61 14.57 10.94 14.86 STD DEV 0.65 1.82 2.35 2.76 0.75 3.06 3.21 3.35 0.72 3.35 NUMBER 30 30 30 30 30 30 29 CONTROL AVERAGE 8.74 7.24 8.92 10.53 10.07 12.09 12.82 13.75 10.70 14.13 STD DEV 0.58 1 52 1.69 1.81 0.50 1.93 1.95 2.13 0.52 2.19 NUMBER 30 30 30 30 30 ,
30 30 CONTROL AVERAGE 8.98 8.05 9.89 11.52 10.27 12.84 13.62 14.59 10.90 14.80 STD DEV 0.82' 2.22 2.39 2.52 0.70 2.64 2.65 2.82 0.69 2 80 NUMBER 30 30 30 30 30 30 30 5/ AVERAGE 9.10 8.37 10.37 12.20 10.45 13.73 14.60 15.59 11.07 15.89 STD DEV 0.65 1.78 2.17 2.70 0.76 2.99 3.17 3.29 0.77 3.43 NUMBER 30 30 30 30 30 30 30 S/ AVERAGE 8.84 7.65 9.49 11.28 10.29 12.66 13.11 13.83 10.74 14.29 STD DEV 0.64 1.82 1.92 2.04 0.40 2.10 2.10 2.27 0.55 2.35 NUMBER 30 30 30 30 30 30 30 6/18 AVERAGE 9.14 8.26 10.19 12.07 10.46 13.45 14.08 15.11 11.05 15.40 STD 1CV 0.67 1.91 2.11 2.46 0.68 2.62 2.74 2.69 0.66 2.68 NUMBER 30 30 30 30 30 30 30 6/ 21 AVERAGE 9.10 8.18 9.98 11.64 10.42 13.25 14.17 15.06 11.01 15.00 STD DEV 0.59 1.64 2.22 2.56 0.74 2.90 3.02 3.25 0.71 3 07 NUMBER 30 30 30 30 30 30 30 7/ AVERAGE 8.75. 7.38 9.23 10.82 10.11 12.10 12.69 13.48 10.65 13.77 STD DEV 0.65 1 71 2.12 2.43 0.71 2.68 2.70 2.84 0.69 2.89 NUMBER 30 30 29 29 29 29 29 9/ AVERAGE 9.06 8.16 9.98 11.56 10.44 13.24 13.95 14.79 10.95 14.93 STD DEV 0.57 1.55 1.80 2.05 0.63 2.48 2.71 2.85 0.67 3.02 NUMBER 30 30 30 30 30 30 30 8/ 26 AVERAGE 8.73 7.22 9.10 10.92 10.09 12.46 13.50 14.37 10.86 14.64 STD DEV 0.67 1.80 2.04 2.29 0.67 2.58 2.60 2.56 0.60 2.74 NUMBER 30 30 30 30 30 30 30 9/ 34 AVERAGE 8.77 7.32 9.17 10.85 10.11 12.17 '3.08 14.01 10.76 14.25 STD DEV 0.58 1.50 1.92 2.15 0.63 2.44 2.47 2.54 0.61 2.59 NUMBER 30 30 30 30 30 30 30 SOLID LINE OUTLINES CELLS WHICH HAVE WEIGHTS SIGNIFICANTLY LOWER THAN ALL CONTROLS.
DASHED LINE OUTLINES CELLS WITH MORTALITY GREATER THAN RAND 0M MORTALITY.
1
F TABLE 14 FROJECT 8 .N UEIGHT/ LENGTH
SUMMARY
. d.sr SPECIES: STEELHEAD ,
METAL: COPPER / ZINC DATE: OCTO KR 1 OCTOKR 15 OCTOBER 26 NOVEMEER 5 NOVEhEER 17 NOVEMBER 30 DECEMBER 7 DAYS: -5 9 20 30 42 55 62
~
AVERAGE LENGTH i5l65i [ENGTH WEIGHT LENGTH W5l55i E555is UEIGHT E5 5i5'U5155i E555I5'Uli55i [555iS'05idili FFB CONTROL AVERAGE 12.78 23.53 27.11 31.06 1 ;.46 34.84 37.85 40.57 15.49 41.37 STD DEV 0.86 5.30 6.71 8.26 1.07 8.82 9.41 10.05 0.31 6.51 NUMER 20 20 20 20 20 20 10
- CONTROL AVERAGE 13.09 24.79 28.66 32.71 14.81 37.63 40.30 43.80 16.01' 46.31 STD DEV 1.08 6.08 6.67 7.36 1.09 8.50 9.04 9.83 1.18 10.41 2
NUMBER 20 20 20 20 20 20 20 CONTROL AVERAGE 12.89 24.08 27.37 31.29 14.42 34.15 37.50 40.31 15.58 42.79 STD DEV 1.09 5.85 5.86 6:54 1.06 7 45 7.93 8.43 1 12 8.75 NUMBER 20 20 20 19 19 19 19 CONTROL A'KRAGE 12.90 23.51 26 64 31.23 14.53 35.10 37.58 40.67 15.60 42.12 STD I(V 0.96 5.54 5.25 5.98 0.89 6.46 6.94 7.48 0.93 7.63 NUMER 20 20 20 20 20 20 20 3/ AVERAGE 13.46 27.11 31.11 36.87 15.18 40.57 43.65 46.84 16.27 49.12 STD IG 0.93 5.31 5.72 7.18 1.17 8.67 9.55 10.47 1.36 10.92 NUMBER 20 20 20 20 20 20 20 5/ AVERAGE 12.61 22.59 26.58 31.54 14.40 35.14 38.22 40.92 15.59 44.80 STD IG 1.26 6.89 7.72 9.19 1 36 9.51 10.31 10.44 1.35 10.99 NUMBER 20 20 20 20 20 20 20 6/18 AVERAGE 13.20 25.87 29.94 35.33 14.96 39.08 42.36 46.13 14.11 49.28 i STD DEV 1.11 6.39 6.71 7.93 1.26 8.98 9.90 10.79 1.38 11.47 NUMBER 20 20 20 20 20 20 20 ,
l 6/ 21 AVERAGE 12.88 24.25 28.86 34.15 14.70 37.85 40.94 43.72 15.73 47.01 i STD DEV 1.08 6 22 7.47 8.72 1.34 9.83 11.16 12.13 1.53 13.31 NUMEER 20 20 20 20 20 20 20 7/ A'KRAGE 12.76 23.24 26.96 31.88 14.44 35.63 38.51 41.97 15.59 44.47 STD DEV 0.84 4.54 5.01 5.84 0.91 6.73 6.98 7.17 0.96 7.40 NUMBER 20 20 , 20 20 20 20 20 9/ AVERAGE 12.93 24.01 27.17 31.93 14.35 34.61 37.52 40.21 15.37 42.55 STD DEV 1.05 5.85 7.62 7.44 0.97 8.05 8.66 9.27 1.01 9.81 NUMPER 20 20 19 19 19 19 19 8/ 26 AVERAGE 12.63 22.66 26.22 31.59 14.49 35.90 39.56 43.13 15.76 46.34 STD DEV 0.98 5.55 6.36 7.83 1.23 9.31 9.97 10.94 1.33 11.22 NUMER 20 20 20 20 20 20 20 9/ 34 AVERAGE 13.13 24.63 27.87 32.82 14.80 36.65 39.48 42.47 15.85 44.85 STD I(V 1.09 5.55 5.53 6.11 1.00 6.89 7.61 8 24 1.14 8.96 NUMPER 19 20 20 20 20 20 20 SOLID LINE OUTLINES CELLS WHICH HAVE WEIGHTS SIGNIFICANTLY LOWER THAN ALL CONTROLS.
DASHED LINE OUTLINES CELLS WITH MORTALITY GREATER THAN RANDOM MORTALITY.
SFECIES: STEELHEAD
=-
WEIGHT / LENGTH SUMMRY (CONTINUED)
- DATE: OCTOBER 1 OCTOBER 15 0CTOBER 26 NOVEM?ER 5 NOVEMBER 17 NOVEMPER 30 DECEMPER 7 DAYS: -5 9 20 30 42 55 62 AVERAGE LE%TH WEIGNT LENGTH WEIGHT LENGTH WEIGNT LINGTH WEIGHT L5NGTH WEIGsi LENGTH'55 PPB 12/ AVERAGE 12.96 23.91 27.61 32.83 14.67 36.63 40.25 43.18 1.06 5.89 15.86 45.7d STD IEV 6.29 7.02 0.94 7 54 8.33 8.51 0.95 NUMBER 20 20 20 20 20 20 20 8.7 13/ 38 AVERAGE 13.37 26.67 31.09 35.97 15.21 40.38 43.68 47.32 STD DEV NUMBER 1.06 5.52 20 20 5.78 20 6.84 1.08 7.51 20 20 7.95 3.49 16.36 1.09 8.?5 20 20 16/ AVERAGE 13.13 24.82 27.76 32.95 14.72 36.69 39.38 42.98 15.86 45.3>
STD DEV 1.06 6.14 6.38 7.81 1.16 9.20 9.54 10.58 1.27 11.tk NUMBER 20 20 20 20 20 20 20 i 16/ 49 AVERAGE 12.90 23.57 27.25 32.13 14.64 36.25 38.97 42.57 15.82 46.10 STD I:EV 1.16 6.58 6.99 8.14 1.26 9.47 9.83 10.54 1.32 11.03 NUMBER 20 20 19(1) 19 19 19 19 20/ AVERAGE 12.98 24.00 26.87 32.29 14.61 35.65 39.18 42.66 15.74 43.50 STD DEV 0.89 4.93 4.97 6.26 0.83 6.42 7.06 7.49 0.87 7.@
NUMBER 20 20 20 20 20 20 20 21/ 71 AVERAGE 13.59 27.32 31.77 37.67 15.23 41.97 45.12 49.18 16.40 51.78 STD DEV 0.65 4.08 5.04 6.29 0.89 7.25 7.73 8.76 0.97 9.31 NUMBER 20 20 20 20 20 20 20 28/ AVERAGE 13.11 25.10 29.25 34.58 14.75 36.88 37.46 40.42 15.49 42.30' STD IEV 1.16 6.89 7.53 8.38 1.14 8.43 7.50 9.00 1.04 8.82<
NUMBER 20 20 20 20 20 19 17(2) i 29/ 78 AVERAGE 13.00 24.07 27.04 32.68 14.80 37.35 41.40 46.47 16.24 50.07 STD DEV 0.98 5.31 5.56 6.37 0.91 6.95 7.24 7.74 0.82 8.52 NUMEER 20 20 20 20 20 20 20 j 31/ AVERAGE 12.79 23.39 26.63 I 33.51 14.84 38.35 42.16 46.49 16.11 49.41-STD DEV 1.04 5.44 5.93 6.55 0.99 7.50 8.18 9.02 1.09 10.25 NUMPER 20 20 l17(1)
, 17 17 17 17 33/ 91 AVERAGE 13.05 24.88 27.33 32.38 14.69 36.50 40.35 44.00 16.00 47.89 STD DEV 1.32 6.64 6.91 7.97 1.20 8.74 9.99 10.69 1.32 11.87 NUMPER 20 19 19 19 19 19 19 51/ AVERAGE 13.11 25.56 29.39 35.06 14.88 39.68 43.17 46.37 16.09 49.35 STD DEV 0.95 5.31 6.36 8.04 1.12 9.23 9.42 9.60 1.17 9.87 NUMBER 20 19 19 19 19 19 19 52/155 AVERAGE 13.02 23.92 24.96 29.52 14.36 34.01 37.33 41.23 15.7243.79 STD DEV 1.04 5.49 5.47 6.34 1.06 7.38 8.20 8.88 1.14 9.64 NUMBER 20 17 17 17 17 17 17 t n (1) FISH JUMPED FROM TANK (2) MOST FISH IN THIS TANK HAD A SEVERE FUNGUS INFECTION WHICH WAS NOT P. RESENT IN OTHER TANKS. I SOLID LINE OUTLINES CELLS WHICH HAVE WEIGHTS SIGNIFICANTLY LOVER THAN ALL CONTROLS.
DASHED LINE OUTLINES CELLS WITH MORTALITY GREATER THAN RANDOM MORTALITY.
l TABLE 15 C0HO PHYSICAL CONDITION PROJECT 8 1
TOTAL ------AVERAGE CONDITION -----
TANK CONCENTRATION (2)TRICH 0DINA SKIN GILL LIVER AIDNEY 9 1 3.0 1.9 2.4 - 3.0 22 1/9 1 4.0 3.3 3.5 4.0 36 1 3.0 3.3 3.9 4.0 48 1/9 3.4 3.5 3.5 4.0 10 5 3.0 3.6 4.0 5.0 45 6/18 2 4.3 3.6 4.0 4.0 32 5 10 4.0 4.2 4.1 4.3 21 6/21 4.0 4.2 5.0 4.5 8 9 6 3.0 3.3 4.5 4.9 33 7 3 4.0 4.0 4.0 3.9 23 8/26 4.0 3.6 4.0 3.5 47 9/34 3.9 3.4 3.9 4.0 34 12 4.0 4.3 4.0 4.5 24 13/38 8 4.5 4.1 4.0 4.7 12 16 4.0 3.8 2.5 3.2 46 16/49 3.2 2.9 2.7 3.3 31 20 3.5 3.2 4.0 3.7 20 21/71 5 4.0 3.8 4.0 4.5 -
7 28 3.0 3.4 4.0 4.5 44 29/78 5 4.5 3.9 4.0 4.1 35 31 4.0 3.5 4.1 3.9 19 33/91 1 3.0 3.1 3.0 3.0 11 51 2.7 2.2 2.5 3.2 43 52/155 3.2 2.3 3.4 2.8 (1) 1.0 = VERY POOR CONDITION 3.0 = AVERAGE HATCHERY FISH 5.0 = PERFECT CONDITION (2) METAL CONCENTRATIONS ARE COPPER, OR COPPER / ZINC IN PARTS PER BILLION
TABLE 16 STEELHEAD PHYSICAL CONDITION PROJECT 8 1
2 TOTAL ------AVERAGE CONDITION ------
. TANK CONCENTRATION GYR00ACTYLUS SKIN GILL LIVER KIDNEY 5 1 2 3.3 3.8 3.5 3.5 18 1/9 6 3.5 3.7 3.6 4.0 28 1 9 2.9 3.5 3.0 3.2 -
42 1/9 7 3.4 3.7 3.5 3.9 !
6 5 9 3.1 3.7 3.2 3.4 1 38 6/18 2 2.9 3.2 3.5 3.4 26 5 3.1 3.0 3.4 3.7 17 6/21 8 3.3 3.5 3.5 3.5 2 9 3.5 4.0 3.5 4.5 27 7 12 3.0 3.7 3.5 3.7 13 8/26 3.0 3.0 3.5 3.5 39 9/34 2 3.0 3.2 2.9 3.5 29 12 10 3.5 3.9 3.5 3.8 16 13/38 3.3 3.2 3.5 3.7 1 16 3.5 4.0 3.5 4.0 l 41 16/49 3.3 3.3 3.4 3.8 30 20 ~
2 3.2 3.3 3.4 3.5 15 21/71 3.3 3.1 3.5 3.2 37 29/73 3.5 3.6 3.5 3.4 25 31 3.1 3.6 3.7 4.0 14 33/91 3.1 3.0 3.5 3.3 4 51 4 3.5 3.7 3.5 3.7 40 52/155 3.4 3.1 3.5 3.3 (1) 1.0 = VERY POOR CONDITION 3.0 = AVERAGE HATCHERY FISH 5.0 = PERFECT CONDITION j (2) NETAL CONCENTRATIONS ARE COPPER OR COPPER / ZINC IN PARTS PER BILLION l
l
- . - - . . - . . . - - . _ - - ~ , ,
TABLE 17 SUMARY OF SUBACUTE (60 DAY) TESTS Water IN-STREAM NO EFFECT LEVEL (PP8)
Temperature Mortality Growth Condition J PROJECT 5 March 15-May 17 7-130C Steelhead Copper 24 45 ---
Zinc 59 >179(I) 4 Coho Copper 24 22 ---
Zinc 220 >326(1) ___
m PROo'ECT 6 May 24-July 28 14-19 C b
Coho Copper 18 21 ---
PROJECT 8 Sept. 30-Dec. 6 14-5"C Steelhead Copper 28(2) >51 III >51 II)
.i k
pgJE:
Highest value tested.
] (2) Based on 3 morta11 ties at 31 ppb; however, there was only 1 mortality at 51 ppb and 28 ppb may be conservatively low.
(3) Statistically, there was no observable effect at the highest concentrations tested (same as steelhead).
'l However, it is possible that the first indications of an effect were present at the highest 3 concentra-tions. There was clearly no effect at 31 ug/l copper or 29 ug/l copper with 78 ug/l zinc.
?
I
~
DRAFT 1 TABLE 18 '
96-HOUR LC50s BY COPPER SPECIES PROJECT 4 5 6 6A 7 i Coho -
l Total 85 90 62 88 103 Dissolved 46 70 50 59 78 Labile 71 81 46 42 67 Steelhead Total 94 90 Dissolved 51 68 l
l Labile 79 57 X Temp. 6.4 7.7 15.5 16.0 15.5 1
1 I
l
- _ _ . _ . , _ _ _ .__..,o_- - - - - - , , _ _ - . . . . _ . . . . . . _ _ _ .- __. .
TABLE 19
. 9-DAY LC50s BY COPPER SPECIES PROJECT 4 5 6 6S(1) 6A 7 Hours Coho Total 73 74 61/61(4) 62 32/84(2) 69 Dissolved 39(3) 58 49/4'9 50 58/60 52 Labile 60 61 45/45 46 38/39 41 Steelhead Total 87 87(2) 55 Dissolved 47(3) 68 42 Labile 73 74 30 kTemp. 6.4 8.3 15.5 15.5 16.2 15.4 .
(1) Dissolved and Labile equations from 6 (2) Estimated by interpolation between one point above and one point below 50% mortality. Mortality too low for Spearman Karber estimate.
(3) Have reservations about value:
a) Only time labile > dissolved b) Calculated labile significantly different from measured.
(4) Fry /smolt
~
DRAFT TABLE 20 CORRELATION OF 96-HOUR AND 9-DAY LC50s WITH AVERAGE WATER QUALITY PARAMETERS C0HO SALMON (96-HOUR) C0HO SALMON (9-DAY) STEELHEAD TROUT (9-DAY)
TOTAL DISS LABILE TOTAL DISS LABILE TOTAL DISS LABILE D.O. -0.338 -0.738 0.250 0.164 -0.484 0.799 0.950 0.378 0.943 TEMP. -0.098 0.231 -0.786 -0.105 0.471 -0.970 -0.980 -0.483 -0.976 HARDNESS 0.004 0.418 -0.654 -0.130 0.613 -0.898 -0.897 -0.246 -0.883 PH 0.164 0.637 -0.421 -0.128 0 689 -0.840 -0.803 -0.067 -0.791 COND 0.194 0.544 -0.537 -0.069 0.597 -0.900 -0.929 -0.320 -0.921 ALKALIN -0.076 0.309 -0.742 -0.114 0.522 -0.953 -0.955 -0.395 -0.949 TSS -0.033 -0.033 0.329 0.072 -0.852 0.591 0.526 -0.306 0.509 AMMONIA 0.167 0.147 0.677 0.341 0.268 0.785 0.792 0.978 0.304 C00 0.581 0.050 -0.204 0.706 -0.030 -0.366 -0.471 -0.977 -0.489 SO4 -0.757 -0.921 -0.204 -0.079 -0.522 0.584 0.931 0.327 0.924 CL -0.423 -0.250 0.231 0.091 0.246 0.632 0.853 0.950 0.863 PB -0.301 -0.353 0.076 0.465 0.274 0.525 0.940 0.869 0.947 CA 0.290 0.772 -0.202 -0.217 0.614 -0.638 -0.339 -0.130 -0.323 MG 0.410 0.729 -0.218 -0.194 0.414 -0.761 -0.965 -0.426 -0.960 K 0.454 0.311 0.290 -0.339 -0.499 -0.149 -0.841 -0.957 -0.352 NA 0.499 0.714 -0.126 -0.196 0.263.-0.704 -0.973 -0.554 -0.990 FE 0.328 -0.199 0.676 0.042 -0.746 0.667 0.427 -0.411 0.407 SIGNIFICANT VALUES (0.05 LEVEL) EXCEED:
+ 0.950 FOR SULFATE 96-HOUR TESTS t 0.997 50R STEELHEAD TESTS t 0.878 FOR ALL OTHER VALUES SI6NIFICANT VALUES OUTLINED D
l l
e
TABLE 21 HIGHEST COPPER CONCENTRATIONS WITH N0 OBSERVED EFFECTS .
DURATION IN DAYS Project 5 Project 6 Project 8 Days 8 29 61 10 30 60 8/9 29/30 61/62(I)
Coho Salmon 22 21 18 18 28 28 33(2,3)
Total 51 22 Dissolved 40 17 17 16 14 14 21 21 24 Labile 43 10 5 4 1 1 8 6 17
- l Steelhead Trcut Total 24 24 24 51 29 29 Dissolved 18 18 18 38 21 21 Labile 16 12 8 32 7 13 (1) Coho /Steelhead (2) Initially, there had been a growth effect, however, th'e fish recovered and caught up their weight.
Had only one measurement been made, this would be the result.
(3) Cu/Zn Tank M
M
TABLE 22 LOWEST COPPER CONCENTRATION WHERE EFFECT WAS OBSERVED Project 5 Project 6 Project 8 Days 8 . 29 61 10 31 61 8/9(I) 29/30 61/62 Coho Salmon .
Effect M G G G( } M M G( } G G Total 78 24 24 33 21 21 31 31 51 Dissolved 18 18 26 16 16 23 23 38 Labile 70 12 9 15 4 4 11 9 36 Steelhead Trout Effect M M M -
(3) g g Total 45 45 45 > 51 31 31 Dissolved 35 35 >38 23 23 Labile 37 30 24 >32 9 15 (1) Coho /Steelhead (2) The growin effect was not statistically significant until later in the test however, inspection of the data makes u', believe that growth had been depressed during the first 10 days (Table )
(3) A mortrlity effect was present with 52 ug/l copper and 140 ug/l zinc. '
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Note: Flows (Ml/ Min) represent those used when diluting 50%
a l FI3URE 2. DILUTER SCHEMATIC
l DRAFT FIGURE 3 CHEHALIS RIVER FLOW (CFS)
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FIGURE 5 l' RAFT CHEHAlls RIVER DISSOLVED OXYGEN D0 13.00t t
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$$$ 8 12.30t
- t t t t t
S t t t St tt 11.60t $2*
-
- tt 2 2 *
- t t 3 2 83 * * * ** t t 228 2122 882 2 * * * ** 82 10.90f $2* * * * $
- 8 182 *28 3 382
- 821 38 tt! 8 t t 12 * **
- 22 *
- tt * $$
- 2
- $2 * **** t** t*2*
- 10.20t * * *t t t
- t t St t t t tt $$$ $
- t
- *
- f
- * *
- tt28 9.50+ ** *
- $2 * *2 3
- *
- t tt 82 2
- 2 21 2 5 8$
8.80t 85 2
- 2 *
- t 8.101 SUBACUTE TESTS 5 6 8 t- - t : =- t t l ACUTE TESTS 3 4 6 6A 7 HH HH H t t-- t- - t-- ---t : --+---t : t---tDATE 0.0 1.2 2.4 3.6 4.8 6.0 7.2 8.4 9.6 10.8 12.0 DATE (0.0 = JANUARY 1: 12.0 = DECEMBER 31) l
FIGURE 6 DRAFT CHEHALIS RIVER PH l PH 8.00t tt 7.80t
- 7.601 *
- 2482 38
- 7.40 ** * *2 82 **
- t 2 2 1388 * * **2t*** St 828482 8 7.201
- 83 3 2322 2 8* $228288 25 t 82
- 8 33232 *233281 * * ** ** *t 3* *22 ** * *t 7.005 22 *t St * * * ** *** t
- t
~
- *
- 28t* **2282 6.80h ** **** **
- * * *
- 2 6.601
- 881
- t SUBACUTE TESTS
, 5 6 8 l l t : -
l t- l l ACUTE TESTS
- 3 4 6 6A 7 HH HH H l t -
1 -
t t . l t --+ +DATE 0.0 1.2 2.4 3.6 4.8 6.0 7.2 8.4 9.6 10.8 12.0 DATE (0.0 = JANUARY 1: 12.0 = DECEMBER 31) <
t
-- -. ,n
FIGURE 7 CHEllALIS RIVER CONDUCTIVITY (AT 25 DEGREES C)
COND 100.01 t
95.01 3
,- t 90.0+ t t
t 85.01 80.01 t
t t 75.0t
- t t t *
- tt
- 70.01 *2*2 *
- t 2 *t 2
- t t 65.0t *
- t
- t t 60.01
- t t
t **
55.0t -
12 t
50.0t SUBACUTE TESTS 5 6 8 l -t t-- l t l ACUTE TESTS 3 4 6 M 7 ft H ft it it t t t-- l l l t l l l tDATE 0.0 1.2 2.4 3.6 4.8 6.0 7.2 8.4 9.6 10.8 12.0 DATE (0.0 JANUARY 1: 12.0 : DECEMBER 31)
FIGURE 8 l CHEHALIS RIVER BINDING CAPACITY (UG/L COPPER)
RIV. BIND 22.01 8 .
- l i
t Ett 20.21 t
_ g .
l 18.4t t 5
- l t t 16.6+
- tt t t t t 14.8t tt t 13.0t
- tt t 1
11.2t t t l ,
t 9.4t
_ t 1 - t 7.6+
- t 5.8t ;
j 3
1 4.01 .
1 SUBACUTE TESTS 5 6 8 l
- +: : : -+
ACUTE TESTS I 3 4 6M 7
. H. H . . .H H . . .H -
l : -
+DATE 0.0 12 2.'4 3.'6 4.8 60 72 8.4 9.6 10.8 12 0 DATE (0.0 = JANUARY 1: 12.0 = DECEMBER 31)
(
F.GU~2 9 '
COHO SALMQN LABILE COPFTR 2 6-HOUR LC50 VS. TEMPERATURE 16.0+ * -
14.0+
~
12.0+
TEMPERATURE -
DEGREES C -
10.0t 8.0+
6.0+
+---------+---------+--------+---------+---------+
- 40. 50. 60. 70. 80. 90.
LABILI CC DER (UG/L)
THE REGRESSION EQUATION If Y= 95.3 - 2.77 X1 ST. DEV. T-RATIO =
COLUMN COEFFIC.I.4T OF COEF. COEF/S.D.
95.20 16.28 5.85 X1 TEMP -2.~~4 1.258 -2.20
- THE ST. DEV. OF Y ABOUT REI:.Ei'EION LINE IS S = 11.94 WITH ( 5- 2) = 3 DEGREE 3 C3 FREEDOM R-SQUARED = 61.8 PERCENT R-SOUARED = 49.1 PERCENT, -3J 'STED FOR D.F.
ANALYSIS OF VARIANCE DUE TO DF EI MS=SS/DF REORESSION 1 693.! 693.3 i<ESIDUAL 3 427.: 142.6 TOTAL- 4 1121.~
- I d pNg FIGURE 10 n COHO SALMON LABILE COPPER 9-DAY LC50 VS. TEMPERATURE 16.0+
- 13.5+
TEMPERATURE DEGREES C 11.0+
8.5+
- 5.0+
+_________+_________+_________+_________+-________+
36.0 42.0 48.0 54.0 60.0 66.0 LABILE COPPER (UG/L)
THE REGRESSION EQUATION IS Y= 76.5 - 2.21 X1 ST. DEV. T-RATIO =
COLUMN COEFFICIENT OF COEF. COEF/S.D.
76.491 4.162 10.38 X1 TEMP. -2.2080 0.3193 -6.92 THE ST. DEV. OF Y ABOUT REGRESSION LINE IS S = 2.958 WITH ( 5- 2) = 3 DEGREES OF FREEDOM R-SQUARED = 94.1 PERCENT R-SQUARED = 92.1 PERCENT, ADJUSTED FOR D.F.
~
ANALYSIS OF UARIANCE i DUE TO DF SS MS=SS/DF REGRESSION 1 418.545 418.545 RESIDUAL 3 26'254
. 8.751 TOTAL 4 444.799
FIGURE 11 STEELHEAD T. ROUT LABILE COPPER 9-DAY LC50 VS. TEMPERATURE TEMP 16.0f 13.5t -
TEMPERATURE -
DEGREES C 11.0t 8.5+
- 6.0t
- ---------+---------+---------;-------- r---------+
- 30. 40. 50. 60. 70. 80.
LABILE COPPER (UG/L)
THE REGRESSION EQUATION IS Y= 111. - 5.17 X1 .
ST. DEV. T-RATIO =
. COLUMN COEFFICIENT OF COEF. COEF/S.D.
110.83 12.51 8.86 X1 TEMP. -5.166 1.164 -4.44 THE ST. DEV. OF Y ABOUT REGRESSION LINE IS S = 7 806 UITH ( 3- 2) = 1 DEGREES OF FREEDOM
~
R-SOUARED = 95.2 PERCENT R-SOUARED = 90.3 PERCENT, ADJUSTED FOR D.F.
ANALYSIS OF VARIANCE DUE TO DF SS MS=SS/DF REORESSION 1 1201.07 1201.07 RESIDUAL 1 60.93 60.93 TOTAL 2 1262.00
APPENDIX A OUALITY COf} TROL AND STANDARD CONDITIONS The analytical quality control program for the bioassay comprised opproximately 20% of the daily sample load. The overall program consisted of the following components.
- Replicate analysis of 10% of the samples processed in the laboratory.
- Routine analysis of internal laboratory standards
- Analysis of externally prepared NDS and/or EPA controls with each group of samples to verify calibration curves.
- Participation in blind EPA performance evaluation.
- Development of precision and accuracy data for all chemical procedures f rom daily laboratory performance.
- Development and maintenance of records documenting performance and maintenance of analytical instruments, water system, and refrigerator system.
- Routine analysis of reagent blanks, diluents, fish food, and sampling vessels for copper and zinc contamination.
Sample Handling and Storage For collecting water samples, various sizes (60-500 ml) of new linear , polyethylene bottles and lids without gaskets were utilized for all chemical tests. Each lot of new bottles was examined for contamination by filling with deionized water acidified to 0.5% with ultrex nitric acid followed after 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> by graphite furnace atomic absorption analysis for total copper and zinc. In addition, two brands of new unwashed 15 ml polypropylene dilution vials (Evergreen and Sarstedt), 1 liter cubitainers (Cole-Palmer Instrument Co.). reused aid washed LPE sample bottles, acid washed 2 ml Perkin-Elmer teflon auto sampler cups, polystyrene disposable 2 ml auto sampler cups, and 50 ml polypropylene syringes were examined in the same manner. Reused bottles were first washed with a detergent solution and rinsed with tap water. The bottles were then immersed in a series of baths consisting of 8 N nitric acid, tap water, 6 N hydrochloric ccid, and tap water in that order. They were then rinsed three times with deionized water and dried. Some zine contamination was evident in reused sample bottles even after the extensive acid washing procedure, thus sample bottles were not reused during the bioassay for zinc analyses. The polystyrene auto campler cups were found to be acceptable for graphite furnace copper analyses, however, they were unsuitable for zine analyses.
All zinc samples analyzed by graphite furnace were placed in A-1
UNAN Parkin-Elmer 2 m1 teflon auto scmpler cups which hcd bacn carried through an acid washing protocol identical to that described for sample bottles except the vials were transferred from bath to bath using teflon tongs and were dried using a heat lamp under a laminar flow hood.
Samples were analyzed, whenever possible immediately after collection. However, because of manpower limitations and the large number of samples, samples were preserved to minimize any chemical changes which might have occurred between the time of l collection and the time of analysis. Any samples not analyzed ;
within the prescribed storage limits were discarded. In all cases, appropriate storage conditions were initiated immediately ,
after collection, l Results of the container contamination study are presented in table A-1. The techniques utilized to preserve samples and the storage time are presented in Table A-2.
Standard Conditions Standard test conditions were established for each of the metals as a function of instrument and metal species. The standard conditions are presented in Tables A-3 through A-5.
Precision and Accuracy Precision and accuracy are both based on comparison of test results with EPA or NBS controls as appropriate. The results are presented in Tables A-6 and A-7.
l Fish Food Analysis In an attempt to assess the contribution of fish food to total copper and zine concentrations in the aquaria, Oregon Moist fish food was analyzed for total copper and zine utilizing a r.ethod modified from Plumb (1981). Fish food was dried to constant weight at 105 degrees C. Two-hundred fifty mg. of dried food was then moistened with deionized water in a 250 ml. flask. Ten ml.
of concentrated ultrex nitric acid was added and the mixture was slowly brought to a boil. Additional acid was added with continued boiling until all visible sign of organic matter was destro ed. After cooling to room temperature, 10 ml of 30%
hydrogen peroxide and 3 ml ultrex nitric acid was added and the flask was warmed over a hot plat.e until all effervescence had ceased, followed by continued boiling to near dryness. The sample was brought to a 10 m1 total volume with 0.5% ultrex i
nitric acid and analyzed by flame atomic absorption spectrophotometry with the results calculated on a ug/gm dry weight basis.
Standard reference materials (NBS orchard leaves 1571, and NBS A-2
rivar codim:nt 1645) and spiked st;mplos were also carried through the digestion and analytical procedures. The results are presented in Table A-8. ,
The floore-Cla rk Company, manufacturers of Oreqon fioist pellets, indicated that their analyses have yielded similar values in the range of 9-10 ug/gm copper and 175-200 uq/gm zine (personal communication with Mr. Larry Lee).
EPA Performance Evaluation The results of EPA's performance evaluation is presented in Table A-9 e
A-3
TABLE A-1 Copper and Zinc Concentrations in Various Washed, Unwashed and Reused Sample Containers Zn Zn Cu Cu n range X range X_
New unwashed 15 ml.
Evergreen dilution vials 10 <1 <1 <1 <1 New unwashed 15 ml.
10 <1 <1 <1 <1 Sarstedt dilution vials Reused, washed 2 ml . teflon auto sampler cups. 10 <1 <1 <1 <1 New unwashed 1 1.
cubitainers 13 <1-2 <1 <1-1 <1 Unwashed 2 ml. polystyrene auto sampler cups 10 <1-8 1.6 <1-1 <1 New unwashed 60 ml. linear playethylene sample bottles 34 <1-2 <1 <1-1 <1 Reused, washed 60 ml. linear polyethylene sample bottles 18 <1-10 1.8 <1-2 0.6 Reused acid washed 150 ml.
polypropylene syringes 20 <1-4 <1 <1-1 <1
TABLE A-2 Bioassay Water Sample Preservation Techniques Parameter Storage Time Preservation Sample Volume (ml) l Total organic carbon 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> 4 C.,H SO ph 2 1000 2 4 Chloride 7 days 4 C. 250 Sulfate 7 days 4 C. 250 Total suspended solids 7 days 4 C. 1000 i l
Chemical oxygen demand 7 days H SO ,ph 2 250 2 4 Total alkalinity 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> 4 C. 250 Total hardness 6 months 0.5%HNO3 4 C. 250 ph 6 hours6.944444e-5 days <br />0.00167 hours <br />9.920635e-6 weeks <br />2.283e-6 months <br /> 4 C. 250 Dissolved oxygen -- -
in situ Total copper 6 months 0.5%HNO3 250 Total zinc " " "
Total calcium " " "
Total magnesium " "
Total potassium " " "
Total sodium " "
Total cadmium "
l- Total lead " " "
i i
Total nickel " " "
l Total iron " " "
Specific conductance - -
in situ Labile copper & zine - -
500 Complexing capacity - -. 500 (Cu & Zn) t
TABLE A-3 STANDARD CONDITIONS FOR FLAME ATOMIC ABSORPTION SPECTROPHOTOMETRY N4F Element Wavelength SBW Id) t(b)
(nm) Flame Matrix (nm) (seconds) Type Matrix Light Cu 324.7 Modifica tion Source 0.7 0.5 air-acetylene 0.5%
HNO hcl c)
Zn 3 213.9 0.7 0.5 " "
Ca -
422.7 0.7 EDL 0.5 " "
Mg 285.2 Lanthanum hcl 0.7 0.5 "
Na "
589.6 0.7 0.5 "
K 766.5 Potassium "
2.0 0.5 "
Fe 248.3 Lanthanum "
0.2 0.5 " "
- n 1
I"} Spectral Band Width (b) Integration Time (c) Hollow Cathode Lamp ,
(d)Electrodeless Discharge Lamp
l I
\
l TABLE A-4 l
STANDARD CONDITIONS FOR GRAPHITE FURNACE AT0f1IC ABSORPTION SPECTROPHOTOMETRY l Sample I
Dry (c) Char IC) Atomize (c) Purge gas Tube Volume Matrix light flow rate (pl)
Wavelength SBW(a) t(b) Matrix Modification source (ml/ min)
Element (nm) (sec)
I (nm) std. 20 2400-0-6 40 hcl
- 110-5-45 700-5-40 0.71 6 0.5%
Cu 324.7 HNO 3 "
" 10 300-5-30 2000-0-6
" 110-5-30 0.71 6
" pyrolytic 30 2n 213.9 650-5-30 2600-1-4
" 130-5-30 4
(NH4 )2HP04 " std. 15 283.3 0.71 2500-1-4 Pb " 130-5-30 650-5-30 0.71 4 (Nil)2HPO 4 4 2500-0-5
" pyrolytic 20 Cd 228.8 900-5-10
" 200-15-5 5
" " 25 232.0 0.21 Ni " 200-10-5 900-5-10 0.71 5 Ca(NO)2 3 Cr 357.9 (a) Spectral Band Width (b) Integration Time (c) Temperature-ramp-hold oHollow Cathode Lamp ,
I s
TABLE A-5 STANDARD CONDITIONS FOR DIFFERENTIAL PULSE AN0DIC STRIPPING VOLTAMMETRY Ij
.. El ement Purge Purge Supporting Cell Initial Final Equilibrate Deposition Pulse Drop Scan Scan Time gas electrolyte type Potential Potential (seconds) Time height Time Increment rate (minutes) (Volts) (Volts) (seconds) (Volts) (seconds) mv. (mv/sec.
C:pper 15 Grade KNO3 teflon .5 0.1 30 60 .05 0.5 2 4 5 0.05R Nitrogen ,
Zinc 15 teflon -1,2 .85 30 60 .05 1.0 4 4
TABLE A-6 PRECISION AND ACCURACY OF ROUTINE WATER CHEMISTRY Parameter True
- n x S.D. Mean Error Relative Relative CV Control Limits Value Error Accuracy Chlcride 87.90 mg/l 4 87.35 1.60 -0.55 63 99.35 1.83 84.7 -
91.1 Chlcride 20.50/mg/l 16 20.68 0.93 0.18 0.88 100.88 4.50 18.82 - 22.5 Chicride 70.20 mg/l 11 70.20 0.74 0 0 100 1.50 68.72 - 71.7 Sulfate 75.0 mg/l 12 75.08 3.23 0.08 0.11 100.11 4.30 68.54 - 81.5 pH 8.60 6 8.43 0.13 .17 -1.98 98.02 1.48 8.35 - 8.8 pH 7.40 4 7.50 .08 0.10 1.35 98.68 1.09 7.34 - 7.7 Hardness 136 mg/l 5 135.88 .50 0.12 0.09 99.91 0.37 134.8 - 137.
Hardness 26.2 mg/l 4 27.65 .47 1.05 3.95 103.95 1.71 25.66 - 27.5 Alkalinity 74.7 mg/l 6 75.1 0.62 0.40 .535 100.54 0.82 73.5 -
75.9 Alkalinity 16.0 mg/l 4 16,95 0.10 0.95 5.94 105.94 0.59 15.8 -
16.2 C0D 10.4 mg/l 6 12.26 2.31 1.86 17.88 117.88 18.84 5.8 -
15.0
- All samples were EPA reference controls
TABLE A-7 PRECISION AND ACCURACY OF TRACE METAL ANALYSES Parameter True n x S.D. Mean Error Relative Relative C.V. Control Limits Value(ug/1) Error Accuracy Zn EPA-60 30 60.90 2.28 0.90 .02 101.5 3.74 56.34 - 6546 Zn EPA-200 216 200.98 2.74 0.98 0.49 100.49 1.36 195.50 -206.46 Zn EPA-400 38 400.37 5.57 0.37 0.09 100.09 1.39 389.23 -411.51 Zn EPA-12 28 11.79 1.34 -0.21 -1.75 98.25 11.36 9.11 - 14.47 Zn EPA-22 6 21.67 1.03 -0.33 -1.50 98.49 4.75 19.61 - 23.73 Zn EPA-16 20 15.95 1.79 -0.05 -0.31 99.68 11.22 12.37 - 19.53 Cu EPA-8 31 8.45 0.85 0.45 5.60 105.62 10.06 6.75 10.15 Cu EPA-250- 13 248,92 2.81 -1.08 .43 99.57 1.13 243.30 -254.54 Cu EPA-40 225 40.82 1.76 0.82 2.06 102.06 4.31 37.30 - 44.34 Cu EPA-11 275 12.28 1.32 1.28 11.67 111.60 10.75 9.64 14.82 Cu NBS-18 202 17.70 1.29 -0.29 -1.65 98.35 7.29 15.12 - 20.28
TABLE A-8
- 'l}
,._ fjWre !
Total recoverable copper and zinc )8
4 4[g[ '
'in Oregon Moist pellets and standard reference materials.
1 Cu ug/gm Zn ug/gm % Cu % Zn Recovery Recovery Oregon Moist 11.75 191.20 99.37 101.87 ]
NBS 1571 11.91 25.8 99.25 103.2 1 Orchard Leaves l NBS 1645 100 1690 91.74 97.22 River Sediment S
0 e
e
-<-_>---__._a_ -
( , 2-07727 U. S. EN /lRONMENTAL P R O T E C TI C, AGENCY b
jeo sr, REGION X g g :r 1200 SIXTH AVENUE g SEATTLE, W A S HING TON 98101 e
~/
UNE M/S 337 December 16, 1982 t Dr. Terry Northstrom W.P.P.S.S.
Fuller Hill Laboratory's Post Office Box 1223 Elma, Washington 98541
Dear Dr. Northstrom:
Enclosed are the results of water pollution performance evaluation, -
study number 9. You are to be commended on the excellent nature of these results. We hope that independent assessments of this kind
. are beneficial to your laboratory.
Sincer ly, Oy'.D .~ --,
Barrygowns,'Clief Regional Quality Assurance Management Office Enclosure b
TABLE A-9
^
- (,, PEPFOHfiANCE LVAndAITUN PEPOPI ,
UA1El12/03/b2
~-
! WA1LA Ps1LLUT10H STUDY NUMPER WP009
! LA80PA10P(8 WAU16 SAMELL PFPnRT TRUE ACCEPIANCE W4FHING PFbt0HMANCE PARAFEILP No!PHEb V A bile VALUE L1Hi rS blPilh LVALUATION l
j TRACE.NE1ALS IN MICPUGHAMS PEP L11ERI l COPPER 1 42 44.7 35./.b3.9 3H.I.51.7 ACCFPIABLt l J 315 336, 209. 379 3no. 30W. ACCEFl'AHLE i '
1 EINC I 22 72.U I2.4*33.2 15.0 3U.6 ACCEPTABLE j J 407 420, 373,-462 384. 451 ACCEPTAHLE j ....................................................................................
i j PINtkAlS 1H HILLIGHANS PER LIIERI (hXCEPT AS NUTFD)
PH= UNITS J 1.0 4."1 3.91 1.10 3.93 4.04 ACCEP1AHLE 4 9,1 9.19 8.92 9.30 W.98 9.32 ACCLP1 Abbe 1
4 SPEC. COND, 1 13 71.0 59.5-63.1 62.1.H0.2 ACCEPTAHLE (UMHOS AT ?$ C) J 792 731. 638.-81). 059. 791 ACC6PrAHLE i TOTAL HAP.DNESS 1 9.2 8.59 3.Hb.13.3 S.02 12.1 ACCT.PTAHLb I (AS CACU3) 2 15H.3 159 144. 166 151. 164 ACCEPTAHLE T01st PLFAI.JNtly 1 ti.H 15.9 12.1./0,1 13.1 19.2 ACCEPIAHLE.
l -(AS CACO 3) 2 69,0 11.2 65.9 7$ 4 00.3 71.1 ACCEPjAHLE Ot;MANDS IN MILLI (iH ANS PEP LIThH:
COD t 17 16.4 9.14 23.0 10.4-21.3 ACCEPIABtn 1 2 346, 286. 392 299.-379. ...
4 s
4 l
APPENDIX B METAL CONCENTRATION DATA General Tables B1-88 summarize the copper and zinc concentrations either as they were directly measured (total, influent, or river binding) or as they were estimated (dissolved, labile, and tank binding) utilizing equations presented in each table. influent measurements are total metal measurements taken from water leaving the diluter prior to entering the test aquaria. The total metal data as well as the data utilized to generate the dissolved and labile equations are graphed in Figures B1-Bll.
The raw labile and binding data for copper and zinc are presented in Tables B9-B10 respectively. The raw data on total, influent, and dissolved-metal are presented by project in Tables B11-B47.
Discussion of Copper Binding During the decay and degradation of plants, animals and mirobial forms of life, groups of organic compounds are formed which possess the ability to form complexes with certain metal ions including copper and zinc. The most widely known and well characterized of these compounds are humic acid, fulvic acid and humin, known collectively as humic substances. These compounds are generally classed as polymeric polysaccharles or polypeptides with molecular masses ranging from a few hundred to several thousand units. They are considered as polyelectrolytes containing a wide variety of functional groups including, most notably, carboxyl, hydroxyl and phonolic groups, which apparently impart the ability to be. alternately protonated and deprotonated under various environmental conditions. Humic acids are defined by their solubility in basic solutions and insolubility in acids and alcohols. Fulvic acids are soluble in both acidic and basic solutions, while humins are insoluble in acidic and basic solutions.
Many factors are associated with the ability of humic substances to form complexes with metal ions as well as the strength of the i bonds joining the two species. These include pH, temperature, ionic strength, presence of competing metal ions, adsorption of ligands onto solids, concentration of ligands and the presence of other competing organic or inorganic ligands. Probably the most significant factor affecting complexation between humic substances and metal ions is pH. Hydrogen ions may compete with metal ions for anionic binding sites on humic substances while dydroyl ions compete with . humic substances for the metal ion.
Raising the pH would then render more binding sites available for complexation and reduce the level of free metal ion. It appears B-1
JRAFT that pH's around neutrality provide the conditions which are most favorable for complex formation. The affect of pH upon copper l complexation with humic acid was verified in our laboratory by I
titrating a 10 mg/l solution of humic acid with copper at pH's ranging between 5.3 and 7.3 (Fiqure 3). A linear increase in
- complexing capacity occured with increased in pH between 5.3 and l 6.7 while a slight decrease occured between pH 6.7 and 7.3.
Increased interest in metal ion speciation has arisen largely because of the recent discovery by many workers that metal ions, including copper, appear to be less toxic or biologically unavailable when they are complexed strongly with hunic l substances. Because of this and other roles in the mobility of l ,
metal ions in natural water sy' stems, humic substances have been referred to as metal-ion buffers. The limit of a natural water system under a given set of environmental conditions to complex a l metal is referred to as the complexation capacity. The term l labile metal refers to that portion of the total metal which has been demonstrated, utilizing a particular analytical techniaue, t to be uncomplexed or weakly so. Specifically, herein, labile metal includes free metal ions plus those complexed metal ions l l which can be disassociated during the analysis to yield free or i 1
electroactive forms of the metal. Non labile metals thus would include those metals which are bound in organic or inorganic complexes or adsorbed onto suspended or colloidal matter.
Several methods have been utilized to distinguish between free
! and complexed metal ion and to determine the complexing capacity I
of a natural water system. These techniques were recently i reviewed by Saar & Weber (1982) and include licuid chromatography-atomic absorption (AAS), ultrafiltration-AAS, dialysis- AAS, ion selective electrode potentiometry and differential pulse anodic stripping voltammetry. All of these methods have inherent disadvantages for use in metal speciation studies. These include most notably equilibrium shifts and adsorption of ligands onto electrodes and membranes. Probably the most widely utilized method in speciation studies has been anodic stripping voltammetry. Much of the current interest in this technique originated from the work of Chau et al (1974) in which they described a " sensitive and accurate method for the
[ measurement of the apparent complexing capacity of lakewater".
Since this original paper, many investigators have applied the same or similar techniques to natural water samples.
With this technique, a mercury drop of uniform size is suspended i from the end of a glass capillary tube and immersed into a sample cell. The sample is stirred at a constant rate while a neoative l potential is applied. The electro-active metal ions diffuse to I the surface of the electrode where they ae reduced and l l After a preselected deposition period, l concentrated in the drop.
the solution is then scanned anodically, wherein the metal is reoxidized and stripped out of the electrode resulting in a polarographic wave. The resulting voltammogram shows peaks which are proportional to the concentration of electroactive metal ions.
l B-2 1
l
~
DRAFT The magnitude of the diffusion current is directly proportional to the concentration of*the electroactive species in the solution and the rate at which the species move from the solution to the electrode surface. To assure that the migration of the electroactive species to the electrode surface is due principally to diffusion and not to electrical attraction, a high concentration of supporting electrolyte must be added to the cell to eliminate any migration current which could form as a result ,
of attraction or repulsion forces between the electrode and the sample solution.
In the differential pulse variation of anodic stripping voltammetry, voltage pulses having constant amplitude are superimposed on a slowly increasing linear voltage ramp. Current measurements are taken just before the pulses are applied and another near the end of the pulse. The difference between the two currents is measured and plotted as a function of potential.
A typical DPAS Voltammogram is illustrated in Figure 4.
Although highly sensitive, DPASV has several drawbacks. As mentioned earlier, high concentrations of supporting electrolyte must be added to the sample which may contribute small concentrationr. of 'electroactive substances which could increase the magnitude of the residual current. Also, dissolved oxygen is readily reduced at the HMDE and can interfere with the determination if not removed. Thus the first step in the analysis is oxygen removal and is accomplished by purging the cell for 10 to 15 minutes with ultrapure nitrogen. There is also evidence that some copper-ligand complexes are electroactive and thus adsorbed onto the electrode surface. This is evident upon examination of voltammograms obtained by titrating humic acid with copper at pH 7. The current often does not. increase
_ linearly frbm zero, as one would expect and the peak potential may vary continuously as the titration proceeds. There may be no concentration of copper for which the peak current is zero and often there is no breakpoint in the titration curve. This behavior was observed by Greter et al. (1979) for both copper and lead. On occasion, secondary copper peaks appear anodic to the' Cu(2+) peak as well as cathodic shifts in the Hg wave which can significantly alter the base line. The latter phenomena were r also observed by Wilson et al. (1980) and ascribed to the l formation of electroactive Cu-fulvic acid complexes and/or of adsorption of humic substances into the mercury electrode. All c of the above phenomena tend to support the existence of
, electroactive copper-humic substance complexes and adsorption of humic . substances to the electrode surface during the plating process and thus copper lability or speciation studies using DPASV must be enterpreted cautiously. However, since the DPASV techniques allow measurements to be made without greatly modifying the sample composition and can be made quite rapidly, it is believed that they may provide at least a rough approximation of the nature of the complexation reactions which 4 occur in natural water systems j
A-3
TABLE B1 j PROICT 3 CGTER SLM1ARY QNTR(L LIN E .LSI EDILM ED. HIGi HIQi DIlllTER A TOTAL 2 62 74 88 118 121 DISS(LVED 1 58 60 71 89 98 l
RIVER EAD BOX CCNTR(L TOTAL 1 2 2 DISS(LE 1 1 DISS(LE CGTER = 0.81 X TOTAL CIPPER: S = 0.14: 8 DATA POINTS PRDICT 3 ZINC SW9tARY C(NTR(L LSI ED.LSI EDIlN ED. HIDI HIEl DILUTER B TOTAL 11 182 364 751 1519 3088 DISS(LVED 2 155 694 2915 RIVER EAD BOX C(NTRIL c TOTAL 2 4 11 l DISS(LVED 2 i i l DISSOLE ZINC = 0.92 X TOTAL IDC: S = I.88: 9 DATA POINTS l
i '?.
i j', U d u bb h Is TABLE B2 l PROICT 4 CGTER SittiARY C(NTR(L LGI ED. LD4 E Illi E . HIDI HIQi DILUTER A TOTAL 3 13 54 63 82 103 i IWLLENT 4 109 DISSG.E 2 23 29 39 ii 55 LABILE I 36 15 53 49 87 DILUTER B TOTAL 3 17 26 33 16 66 INLLENT 4 71 DISSG.YED 2 9 14 18 25 36 LABILE I li 21 27 38 55 DILUTER CI TOTAL 3 18 21 33 12 59
. IWLLENT 4 64 DISS(LVED 2 II 13 18 23 32 LABILE I 15 21 28 36 51 RIVER EAD BOX CG(TR(L OTER TMS TOTAL 2 4 3 l DISS(LYED 2 2 LABILE BMM 4 1 x DILUTER C FAILED AFTER AN ESTMTED 89 H0lRS. T0XICANT CONCENTRATION HAS APPROXDiATELY 25Z OF TE STATED CGENTRATION AT 96 H3RS.
l DISS(LVED C(PPER = 0.51 X TOTAL CG9ER: S = 0.09: 13 DATA POINTS LABILE C(PPER = -0.86 + (4.85 X TOTAL CGfER): R SalARED = I.99: 9 DATA POINTS i
- D, D $, mIY .
u.) e E '. 3 U TABLE B2 (contd.)
FMkECT 4 ZDC SurrtARY CONTRE. LOW ED. LOW EDIlM HED. HIW HIGH DILUTER B TOTAL 8 326 188 717 969 1383 IWLLENT 5 1964 DISSQ.9 9 288 423 632 855 1220 LABILE 8 264 389 581 786 1122 DILUTER D TOTAL 8 386 658 1862 1768 2919 INLLENT 5 2838 DISSE.E 9 348 573 636 1560 2602 LABILE I 313 527 861 1933 2391 RI E EAD BOX CONTRG.
. TOTAL 3 5 8 DISSQ.VED 9 1AILE 5 BDGING I 8 DISSE.VED IDC = 0.88 X TOTAL ZDC: S = 0.03: 9 DATA POINTS LABILE ZDC = 0.81 X TOTAL ZDC: S =0.11: il DATA POINTS i
i
- - - - - + ,, ..- , _ . . , -
-__,y _.._.._.,,.,,_,- - -. 7__ . _ . _ _- . , .. - . , , - - . - - - . _ _ - - .
c.- v - . .
. a TABLE B3 FROICT 5 CIPPER SlitMRY C(HTRG. LOW E.134 EIlm E . HIQi IEQi DILLITER A TOTAL 3 9 16 24 51 89 IWLLENT 2 1B 16 26 94 DISSG.E 1 7 12 18 il 69 LABIII I 8 3 18 34 68 DILUTER B TOTAL 3 7 12 22 15 78 IWHENT 2 8 12 75 DISSE.E 1 5 9 17 35 61 LAIII I I I 8 29 58 RIVER EAD BOX CCHTR(L OTE TAES TOTAL 2 2 3 DISSE.E 1 1 LABIll G I BIEDC 12 11 DISSE. E CIPPER = 1.78 X TOTAL CCPPER: S = .12: 49 DATA PGINTS LABILI C(PPER = -11.48 + (C.89 X TOTM. COPPER): R SalARED = 0.94: 15 DATA POINTS
,w - w - --- , e. ,,.m, - - . . , . -
m -, - - ----.,,a
O D' O (Y ;
. . . .>>a TABLEB3(contd.)
PRO ICT 5 ZINC SlfttARY CONTRG. LOW E . LOW EILM E. HIW HIW DILilTER C TOTAL 11 59 93 179 326 612 i D RLENT 3 38 80 177 613 l DISSG.E 11 44 69 132 241 564 l LABILE 18 48 75 115 264 569 l DIlllTER D TOTAL 10 28 il 66 119 220 l D RLENT 3 ~11 38 73 207 l DISS(LVED 11 21 30 49 88 163 l LABILE il 23 32 53 96 178 i RIVER EAD BOX CG(TRG. OTE TMS TOTAL 2 3 11 !
DISSG.E 2 11 )
LABILE 4 il BDEDE I 8 0 DISSG.E IDC = I.74 X TOTAL IDC: S = 0.19: 14 DATA POINTS LABILE ZDC = 0.81 X (TOTAL ZDC): S = 0.11: 19 DATA PGINTS ACTUAL YALLES ARE REP (RTED FOR DILUTER C, HIGH CONCENTRATIN. TESE FENTS ERE ET UTILI2ED IN ANY (F TE CALCILATIONS ON THIS PACE.
l l
l
TABLE B4
/
PfEECT 4 ETE W
'- 'l (XPPER COURS. LOW E.LIN EDIIM ED. HIQi HIQi DILUTER A TOTAL 2 39 51 77 97 149 IFLtENT 2 il 50 153 DISS(L E 1 32 il 62 ' 79 121 LABILE I 25 36 60 78 97 i DILilTER D TOTAL 2 33 49 83 113 238 IWLLENT 2 33 55 258 DISSOLVED 1 27 il 67 116 193 LABILE I 19 34 65 121 217 RIVER EAD BOX CCHTR(L OTER TAES TOTAL 2 2 2 DISS(L E 1 LABILE I I BDCDC 12 14 11 DISS(LVED C(PPER = 1.81 X TOTAL COPPER: S = 0.88: 11 DATA POINTS LleILE CIPPER = -18.8 + (1.92 X TOTAL C(PPER): R SQUARED = 1.II: 5 DATA POINTS ZDC CONTRG. LOW ED. LIN EDIIM ED. HIGi HIGl DILt!TER D TOTAL 8 98 117 232 371 612 IWLLENT i 84 lit 598
- DISS(LVED 7 81 132 209 331 551 l LABILE 7 81 132 209 334 551 l
RIVER EAD 80X CCNTR(L TOTAL 2 4 8 LABILE 5 BDCDC 0 8 DISS(LVED ZDC = 0.9 X TOTAL IDC: S = 1.08: 3 DATA POINTS LABILE ZDC = 8.9 X TOTAL ZDC: S = 0.81: 2 DATA POINTS
., g P) 9 ,y-.
TABLE B5 F90ICT 6A ACUTE StINRY
- '"J J d C0FfER C MRG. LOW ED. LGi EIIM ED. HIGH HIQi DILUTER A TOTAL 2 38 48 66 88 96 IWLLENT 3 38 18 96 DISS(L E 2 27 34 17 57 68 LABILE I 28 29 17 68 76 DILilTER D TOTAL 2 13 53 78 88 1H IEllRI 3 12 51 119 DISSOL E 2 27 38 50 62 71 LABILE I 28 31 51 68 81 RI E EAD BOX CMROL OTER TAES TOTAL 2 3 2 DISSG.YED 2 LABILE I I BD0DE 16 15 17 DISSE.E COPPER X 1.71 X TOTAL C0ffER: S = 0.17: 8 DATA POINTS LABILE C'FPER = -16.88 + (1.67 X TOTAL C(PPER): R SGMRED = 1.00: 5 DATA POINTS ZINC CGITR(L LSI ED. LSI EDILM ED. HIQi HIQt i
DIllliER D TOTAL 8 109 132 154 186 233 IELIENT 1 III 121 233 DISSG.E 5 96 116 136 164 205 l LABILE I 98 119 139 167 218 l
1 RIVER EAD BOX CGITR(L TOTAL 2 1 8 DISSEL E 5 LABILE 5 BDODE I I DISSG.VED ZDC = .88 X T01AL ZINC: S = 0.03: 3 DATA POINTS LABILE ZDC = 8.9 X TOTAL ZINC: S = 1.05: 3 DATA PO N S
D. 't
'i :R -,3.W du -mj ;d .
fj TABLE B6 FinECT & CIPPER Sit 99RY (SLEMCUTE TESTS)
CGG E. LOW MED. L(N EDILM E. HIGH HIGH DIllHER B TOTAL 2 5 9 18 33 65 IWLLENT 2 5 11 66 DISSG.VED 1 4 7 li 26 51 LABILE I I I i 15 44 DILUTER C TOTAL 2 8 13 21 34 53 IWLLENT 2 8 15 61 DISSG.VED 1 & il 16 27 41 LABILE I I I i 16 33 RIVER EAD BOX CGGG. OTER TMG TOTAL 2 2 2 DISSG.VED 1 LABILE I I BDSM 16 li 15 DISSG.VED CG9ER = 1.78 X TOTAL CG9ER: S = 0.12: 19 DATA POINTS LABILE CIPPER = -15.27 + (1.91 X TOTAL CGTER): R SEMRED = 0.97 17 DATA POINTS l
l l
l l
t l
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., D f. I Y s 1,' -
l C. . . .!
. ; J ;
TABLE B7 PRDICT 7 CCPPER SlfttARY CCNTR(L LSI E. L(N EILM E. HIGH HIGH DILUTER A TOTAL 1 46 64 75 96 121 IElBE 2 62 74 ill DISSILVED 1 35 46 57 73 92 LABILE I 23 34 15 61 84 DILllTER B TOTAL 1 18 38 17 78 134 I EtENT 2 29 54 '84 DISS(L9ED 1 14 23 36 59 112 LABILE I 2 11 24 18 90 DILUTER C TOTAL 1 18 29 18 81 112 DRLENT 2 29 53 87 DISS(LVED 1 14 22 36 62 188 LMILE I 2 11 25 54 96 DILUTER D TOTAL 1 19 57 73 86 113 I E1ENT 2 61 71 95 DISS(L9ED 1 37 13 55 65 06 lAILE 8 26 32 ii 54 74 RIVER LEAD BOX CCNTR(L OTIER TAWS TOTAL 1 2 1 DISS(LVED 1 LMILE I I BIl0DC 18 16 12 l
DISSELVED C(19ER = 0.76 X TOTAL CIPPER: S = 0.11: 23 DATA PGINTS i LABILE CCPPER = -11.51 + (0.76 X TOTAL CIPPER): R S D RED = I.91: 1 DATA POINTS
l
.I ; f I
.- . 1 a
TABLE B7 (contd.)
l PRDICT 7 ZINC S(MtARY C(HTRG. LOW E. LIN MEDILM ED. HIGH HIGH l
DILUTER B TOTAL 13 81 108 118 266 385 IWLLENT 3 87 146 227 DISSG.E 68 91 124 223 323 LABILE 70 94 129 231 335 DILUTER D l TOTAL 13 lit 171 215 273 349 IWLLENT 3 164 195 247 DISSE.YED 118 146 181 229 293 LABILE 9 122 151 187 237 304 RIVER EAD 80X C(NTR(L TOTAL 3 3 13 LAILE 4 9 DISS(LVED ZDC = 0.81 X TOTAL ZDC: S = 0.23: 9 DATA POINTS LABILE ZDC = 0.87 X TOTAL IDC: S = 1.06: 3 DATA POINTS L
l l
I
D$
TABLE B8 -
.a u PROICT 8 C0PfB Stit%RY CGfTRG. LOW E . LIN EIIM E. HIQi IEH DILLITER A TOTAL 1 5 9 16 28 51 DFLIENT 1 8 15 Si DISSG.E 1 4 7 12 21 38 LABILE I I/Is I/I I/5 6/18 31/12 DIL1JTER 8 TOTAL 1 6 8 13 21 33 IIFlisti 1 8 13 J DISS(LE 1 4 6 II 16 24 LABILE I I/I I/I I/2 1/11 11/23 DIL11TER C TOTAL 1 5 7 12 21 31 DFL1ENT 1 3 8 12 33 DISSE.E 1 4 5 9 15 23 1 MILE I I/I I/I I/1 1/10 9/21 DILITTER D TOTAL 1 6 9 il 29 52 DFL1ENT 1 5 9 16 Si DISS(LE 1 4 7 12 21 38 LABILE I I/I I/I I/5 7/19 31/13 RIVER EAD 80X CGITRG. OTIER TA*S TOTAL 1 1 1 DISSE.E 1 LABILE I I BDODE 21/12 24 25/11 FIRST HN.F 0F TEST / SECGC HALF F TEST DISSE.E COPPER = 1.71 X TOTAL CG9ER: S = 1.13: ili DATA POINTS FIRST HALF 0F TEST:
LAILE CCPPER = -21.51 + (1.17 X TOTAL CCPPER): R SalARE = I.89: II DATA POINTS SEED O HALF F TEST:
LABILE CG9ER = -11.24 + (1.14 X TOTAL C(PPER): R SQUARE = 0.82: 15 DATA POINTS
.O.
- O ? V., T, TABLE 88(contd.)
l PRD ICT 8 I M SIMtARY CCNTRE. L(N tED. L(N EILM E .IGGH HIGH l
DILUTER B TOTAL 9 21 26 38 71 91 I ElENT 2 13 16 27 75 DISSIL E 3 17 21 30 57 73 !
LMdLE 8 18 22 32 68 76 l DILIITER D TOTAL 9 18 31 49 78 155 I ElENI 2 18 36 63 131 DISS(LWD 3 14 27 '39 62 121 LABILE 8 15 29 il 66 138 REER LEAD BOX C(NTR(L TOTAL 1 2 9 DISS(LWD 3 LABILE 3 8 BMM I O DISS(LB IM = 0.80 X TOTAL ZM: S = 0.88: 52 DATA POINTS LABILE ZDC = 1.01 X TOTAL ZDC: S = 0.11: 27 DATA POINTS l
l
w TABLE B-9 LABILE COPPER AND RIVER BINDING RIVER THK RIVER i P MMDD SEQ TK CUL CB CUT PH PH 4 0223 42 8 66 5 74 6.8 6 4 4 0223 42 10 39 5 50 6.8 6.6 4 0223 42 11 101 5 122 6.8 6.6 4 0223 42 20 0 5 3 6.8 6.6 4 0223'42 22 46 5 55 6.8 6.6 4 0223 42 23 19 5 30 6.8 6.6 4 0223 42 24 17 5 19 6.8 6.6 4 0223 42 33 27 5 32 6.8 6.6 4 0223 42 34 14 5 17 6.8 6.6 4 0223 42 35 49 5 54 6.8 6.6 5 0325 90 2 15 7 23 6.7 7.2 5 0325 90 5 72 7 81 6.7 7.2 5 0325 90 6 0 7 8 6.7 7.2 5 0401 99 20 0 11 1 7.0 7.2 5 0401 99 22 57 11 74 7.0 7.2 5 0401 99 23 5 11 19 7.0 7.2 5 0408 108 13 0 14 3 6.9 7.2 5 0408 108 14 0 14 8 6.9 7.2 5 0408 108 17 12 14 23 6.9 7.2 5 0408 108 18 58 14 74 6.9 7.2 8 0415 115 13 0 14 3 7.0 7.1 5 0415 115 14 0 14 9 7.0 7.1 5 0415 115 17 10 14 24 7.0 7.1 5 0415 115 18 75 14 90 7.0 7.1 5 0415 115 38 0 14 3 7.0 7.1 5 0422 122 20 0 17 6 6.8 7.1 5 0422 122 22 49 17 89 6.8 7.1 5 0422 122 23 17 17 35 6.8 7.1 5 0422 122 24 0 17 7 6.8 7.1 5 0429 129 13 0 12 6.9 7.1 5 0429 129 14 8 12 6.9 7.1 5 0429 129 17 3 12 6.9 7.1 S 0429 129 18 50 12 6.9 7.1 5 0506 136 8 8 12 24 6.9 7.2 5 0506 136 10 0 12 11 6.9 7.2 5 0506 136 11 60 12 83 6.9 7.2 5 0506 136 12 0 12 1 6.9 7~.2 5 0513 143 2 20 19 37 7.0 7.0 5 0513 143 3 0 19 4 7.0 7.0 5 0513 143 5 64 19 85 7.0 7.0 5 C513 143 6 2 19 22 7.0 7.0 6 C528 151 1 55 12 72 6.9 7.3 6 0'2E 151 4 118 12 141 6.9 7.3 6 (628 151 5 0 12 2 6.9 7.3 6 0528 151 6 28 12 42 6.9 7.3 6 0528 151 38 17 12 31 6.9 7.3 6 0528 151 40 195 12 230 6.9 7.3 6 0528 151 41 63.12 80 6.9 7.3 6 0608 167 19 43 18 7.2 7.1 6 0608 167 21 0 18 7.2 7.1 6 0608 167 22 0 7.2 7.1 6 0608 167 24 0 7.2 7.1 6 0614 176 43 76 16 108 7.2 7.8 6 0614 176 45 20 16 38 7.2 7.8 i 6 0614 176 46 59 16 78 7.2 7.8
- 6 0622 185 19 48 16 48 7.3 7.9
- 6 0630 193 25 44 15 74 7.4 7.4 6 0630 193 29 0 1S 17 7.4 7.4 6 0707 200 32 0 17 8 7.1 7.2 6 0707 200 35 33 17 52 7.1 7.2 6 0713 206 19 45 14 59 7.1 7.3 6 0713 206 21 0 14 7 7.1 7.3 6 0713 206 22 0 14 3 7.1 7.3 6 0713 206 24 0 14 20 7.1 7.3 6 0721 214 19 35 15 59 7.2 7.3 6 0721 214 24 3 15 18 7.2 7.3 6 0727 220 34 3 21 22 7.0 7.1 6 0727 220 35 27 21 60 7.0 7.1
TABLE B-9 (CONTINUED)
LABILE COPPER AND RIVER BINDING (CONTINUED)
RIVER THK RIVER P MMDD SEQ TK CUL CB CUT PH PH 7 0920 233 25 90 18 148 7.2 7.3 7 0920 233 26 0 18 18 7.2 7.3 7 0920 233 29 22 18 48 7.2 7.3 7 0920 233 40 89 18 112 7.2 7.3 7 0920 233 42 0 18 1 7.2 7.3 8 1007 248 1 0 21 16 7.2 7.4 8 1007 247 4 37 21 56 7.2 7.4.
8 1007 247 5 0 21 1 7.2 7.4 8 1007 247 6 0 21 5 7.2 7.4 8 1007 247 14 16 21 37 7.2 7.4 8 1007 247 16 0 21 13 7.2 7.4 8 1007 247 17 0 21 7 7.2 7.4 8 1013 253 25 7 21 27 6.9 7.2 8 1013 253 26 0 21 4 6.9 7.2 8 1013 253 29 0 21 10 6.9 7.2 l 8 1013 253 38 0 21 4 6.9 7.2 8 1013 253 40 25 21 43 6.9 7.2 8 1013 253 41 0 21 13 6.9 7.2 8 1013 253 42 0 21 1 6.9 7.2 8 1020 260 19 10 21 32 7.0 7.3 8 1020 260 21 0 21 6 7.0 7.3 1 8 1020 260 24 0 21 12 7.0 7.3 I 8 1020 260 35 11 21 31 7.0 7.3 8 1020 260 36 0 21 1 7.0 7.3 8 1027 267 25 6 22 37 6.9 7.0 8 1027 267 40 32 22 53 6.9 7.0 8 1103 274 19 11 20 33 6.7 6.7 8 1103 274 21 0 20 8 6.7 6.7 1 8 1103 274 22 0 20 1 6.7 6.7 I 8 1103 274 24 0 20 15 6.7 6.7 l
. 8 1103 274 32 0 20 9 6.7 6.7 )
8 1103 274 34 0 20 17 6.7 6.7 4 8 1103 274 35 15 20 38 6.7 6.7 l 8 1110 281 14 14 15 38 6.8 6.9 )
8 1110 281 16 0 15 16 6.8 6.9 8 1110 281 17 0 15 6 6.8 6.9 )
8 1110 281 18 0 15 1 6.8 6.9 l
8 1110 281 25 4 15 27 6.8 6.9 8 1110 281 26 0 15 4 6.8 6.9 8 1110 281 29 0 15 11 6.8 6.9 8 1115 286 32 0 16 6 6.7 6.9 8 1115 286 34 0 16 15 6.7 6.9 8 1115 286 35 27 16 31 6.7 6.9 8 1115 286 43 35 16 46 6.7 6.9 8 1115 286 45 0 16 6 6.7 6.9 8 1115 286 46 0 16 17 6.7 6.9 1 8 1115 286 48 0 16 1 6.7 6.9 I 8 1123 294 32 0 11 6 6.7 6.8 8 1123 294 34 3 11 15 6.7 6.8 8 1123 294 35 26 11 38 6.7 6.8 8 1123 294 43 45 11 56 6.7 6.8 8 1123 294 45 0 11 8 6.7 6.8 8 1123 294 46 7 11 17 6.7 6.8 8 1123 294 48 0 11 3 6.7 6.8 8 1201 302 32 0 8 5 6.8 6.9 8 1201 302 34 9 8 17 6.8 6.9 8 1201 302 35 24 8 32 6.8 6.9 8 1201 302 43 51 8 52 6.8 6.9 8 1201 302 45 0 8 6 6.8 6.9 8 1201 302 46 9 8 17 6.8 6.9 8 1201 302 48 0 8 3 6.8 6.9 i 8 1207 308 38 0 9 9 6.4 6.6 l 8 1207 308 40 47 9 50 d.4 6.6 8 1207 308 41 9 9 18 6.4 6.6
TABLE B.-10 LABILE ZINC TANK RIVER P MMDD SEQ TK ZNL ZNT PH PH 4 0223 42 2212901380 6.8 6.6 4 0223 42 23 600 676 6.8 6.6
- 4 0223 42 24 263 333 6.8 6.6 4 0223 42 31 175 209 6.8 6.6 4 0223 42 33 221 297 6.8 6.6 4 0223 42 34 147 154 6.8 6.6 4 0223 42 35 475 551 6.8 6.6 4 0223 42 43 211 324 6.8 6.6 4 0223 42 4618232578 6.8 6.6 4 0223 42 47 702 932 6.8 6.6 5 0325 90 26 592 455 6.7 7.2
[ 5 0325 90 27 61 83 6.7 7.2 5 0325 90 29 175 198 6.7 7.2 5 0401 99 43 11 18 7.0 7.2 5 0401 99 46 181 207 7.0 7.2 5 0401 99 47 52 58 7.0 7.2 5 0408 108 37 28 31 6.9 7.2 5 0408 108 38 188 229 6.9 7.2 5 0408 108 40 46 62 6.9 7.2 5 0415 115 37 21 39 7.0 7.1 5 0415 115 38 175 226 7.0 7.1 5 0415 115 40 48 59 7.0 7.1 5 0422 122 43 24 30 6.8 7.1 5 0422 122 46 209 223 6.8 7.1 5 0422 122 47 57 62 6.8 7.1 5 0506 136 33 157 172 6.9 7.2 5 0506 136 34 27 36 6.9 7.2 5 0506 136 35 530 611 6.9 7.2 5 0506 136 45 32 37 6.9 7.2 5 0513 143 26 594 631 7.0 7.0 5 0513 143 27 42 56 7.0 7.0 5 0513 143 29 160 176 7.0 7.0 6 0528 151 38 63 78 6.9 7.3 6 0528 151 40 569 591 6.9 7.3 6 0528 151 41 195 211 6.9 7.3 6 0614 176 38 98 99 7.2 7.8 6 0614 176 43 214 238 7.2 7.8 6 0614 176 45 106 116 7.2 7.8 6 0614 176 46 140 164 7.2 7.8 7 0920 233 38 126 139 7.2 7.3 7 0920 233 40 274 343 7.2 7.3 7 0920 233 41 194 216 7.2 7.3 8 1007 247 14 80 95 7.2 7.4 8 1007 247 17 21 24 7.2 7.4 8 1013 253 38 26 31 6.9 7.2 8 1013 253 40 114 146 6.9 7.2 8 1013 253 41 43 46 6.9 7.2 8 1013 253 42 25 35 6.9 7.2 8 1020 260 19 71 79 7.0 7.3 8 1020 260 21 13 15 7.0 7.3 8 1020 260 24 44 47 7.0 7.3 8 1027 267 38 33 36 6.9 7.0 8 1027 247 40 144 151 6.9 7.0 8 1027 267 41 53 63 6.9 7.0 I 8 1103 274 19 54 82 6.7 6.7 l 8 1103 274 21 50 50 6.7 6.7 8 1103 274 24 26 44 6.7 6.7 8 1110 281 14 83 96 6.8 6.9 8 1110 281 16 34 42 6.8 6.9 8 1110 281 17 11 13 6 8 6.9 8 1115 286 43 149 162 6.7 6.9 8 1115 286 45 15 21 6.7 6.9 8 1115 286 46 38 56 6.7 6.9 8 1123 294 43 150 171 6.7 6.8 8 1123 294 45 23 32 6.7 6.8 8 1123 294 46 39 45 6.7 6.8 8 1201 302 43 138 146 6.8 6.9 8 1201 302 45 22 21 6.8 6.9 8 1201 302 46 35 47 6.8 6.9
_ II
..,E _
DRAFT t
MLUTER A DILUTER 3 DILUER C BILWTER 3 N I t il it il . E 9 EN C MN L E N ML W C C L MEN N EC WN N L C N L EN MEC E L N WL N EN WC L MEN N C 4 E I ? 1 4 L.6 7 0 9 10 ll_t2 lLl4 15 16 lLit_It 20 21 95 st St 25 24 2L28 29 M_31 32_33 34 M 1A 3L38 39 40 41_42_4L44 4LM 4L40 3 e l$ Cut 02 2 4 74 93 3 98 122 53 49 94 102 65 173 2 1 156 46 31105114 St I f3 47134114 3 2 14tutt2 l'5 69 54 32 et 45121 tel 75 90 52 IM 114 1 2005102 l'4 H 90 116 127 57 N 2591022324 28 2X5It2 l' 7 32 M M ", 54 lit 73 56 73 37 IM 100 1 24CW182 2 8 73 87 2 Ile 99 H 73 90125 M 107 2 137 66 39 76 114 57 8 Il 39 146 116 2 8 290n82 2 9 70 96 120127 M 95 1 134 48 H 112 32 1 3tCUT82 210 M 70 99 ill 62129 83 58 1 83 45 142 122 3Kule2 211 H lit 122 let M 114 2 1 35tuf02 212 el 67 82115 H 126 2 1 34C5182 213 et 11 129 se 37tuit2 214 GI 18 let te TAILE D-12 P90KCT 43 19IE CWPER R
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PROICT 31 IOTE Zllt t
DILUTER A DILNTER B DELUTER C BILuiEN 9 N I l 11 ft :4 4 E e EN C 101 N L EN 101 L N C C L III E R H E C 151 N N L C N L EN 101 E C N L N 181 L N K N 101 C L lel 1 N N C & (
I ? 3 4 5 6 7 I 9_.10 11_l2__13_l4 15 lLILle lf 20_21 22 23_24_25 2L2L2O_2f_.30 31 32 33 34 W34 3L3B 39 40_.41 42 43_.44 45 4L4L40 I B 15ZNT32 2 4 1771439 349 N02fM 349 14492953 M7165 2421605 371 5931032 2221022 36617M 434 16ZN102 2 5 474 3M 13922799 684 IM 604 2391107 3921831 647 20ZN182 2 4 1711647 453 0341397 834 7 2141536 674 5501004 549 3 23N192 2 7 752 344 1502M57 735179 370 2821340 4832250 833 13 2 24NT82 2 0 20 1961555 374 7503051 351 21500M70 730194 3342407 568 8671494 3171431 SM2374 867 4 3 29ZNIO2 2 9 2031655 400 8423324 Sie 344 5479061627 912 5 3 '
312 nit 2 210 795 383 14253319 783 202 942 3101559 5532598 927 332N182 211 1571414 355 6822992 682 1833309 390 8371720 19 825 3 T6ILE B-29 P90KCT 48 191E ZINC 1
DILUTER A DILUTER B DILUTER C DILUTER S N I t it il 64 i E V EN C 191 N L E il 151 L N C C L 181 E R H E C 101 N N L C N L EN lel E C N L N 101 L N EN III C L 191 E N N C A f
_1 2 3 4 5 .& 7.__B t 10 ll_.12_.13 14 15 lLlL18 19 20 21_.22 23 24_.25_.26._2L28 2f__30_.31_.32 33 34._35._34 37 M 19 40 41 42 43._44 45 46_.4L48 9 R 39ZNT82 222 38 329 964 403 6421358 479 21 9501351 609 324 34 583 159 203 305 406 222 25 315 149 560 402 12 II & 4 42M182 223 470 f471380 676 333 209 2t? 154 551393 45N182 224 319 977 482 4901383 12 556 156 222 291 392 2 .
6 ZNit? 225 12 321 972 478 6821384 554 I 9751379 491 329 13120 32 52 M 86 N 14 71 42 92 113 5 517N!82 226 le 400 9721344 694 329 9 0 542 Nit? 227 10 3461040 490 8311307 330 4 56ZNT82 228 9 593 10101435 ::33 372 4M 492 40530M ll0018M 3 3911040 M329901110 2 2 592NI82 229 322 998 485 7251424 le 20 401 127 151 234 293 155 4 210108 397 294 3782054 See 9901714 4 3 42Zuft? 3 2 37717M 6%30061064 9 3 4 24182 3 3 1942093 44410771741 5 3751732 44620951044 le 3 7 482 Nit 2 3 4 3901754 65e28941026 7 3 2 712Nis? 3 5 3732e92 64310021707 5 4 3 74ZNT92 3 j 3011394 63729291092 3 3 2 752NI82 3 i 3092947 65014498743 7 1 3 762N102 3 9 3171050 44030221117 9 24 le 7724382 3 9 3732977 6451050lM2 7 4 GZNIO2 310 405t?!? 64328951059 4 1 1 N
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. si 1AILE t-30 PROICT $1 18it Illt t
BILuiER A I!LUTER I DILUTER C ttLUTER 3 N. I E N C les N L EN lut L N C C L M E il N EC lut N N L M L EN 158 E C N L N L M EN 150 C L III E N N C &
l 2 3 4 5 6 7 8 t_IO !l.12 !3 I4_15 ILiLIS tL20 21 22 23 24 25 26 2L29 29 N 31 32 33 34 35 3L3L38 39 40 4l 42 43 44 45 M 47 48 9 R 83Zuit2 318 847uis2 319 29 H2 St its 216 3M 163 43149 M M2 311 2? 210 32 59124 7 19105 37 M7 55 5 13 3 89 29 871 Nit 2 322 10 ft 48109 44 578 367 88N102 323 27137 44 254 to le il luit2 324 32 ett 48 94 240 354 41252 M H IM 11 4 p'ZNIB2 325 482 H 153 274 354143 22107 71642 341 34 259 62 12 114 2 25 157 47 231 72 12 3 2 i
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148LE 8-31 F88KCT 61 181E ZINC R
SILUTER 4 SILUTH 8 IILUTER C TiLUTIR 8 N 1 1 46 6 il 4 E V N EMN C L M1 L M N EN MN L C N 181 L C ER N L E C N IBl 808 L EN N C lel L EN N C N let L N KC & E 1 2 3 4_5 6 7 4 Cs f 10 11 12 13 14 15 16 lL18 It 20 21 M 23 28 M 9A M 28 29 30 31 32 3L34_35 36 3L38 39_40 41. 42. 43 44 45 M 4L48 8 a 1517NI82 528 12 354 78 124 591 211 3 572 349 87 218 135 7 1 1 153N!82 529 28 eel 344 94 233 148 1 (1 1552N182 538 11 375 N 214 589 217 14 157Nf82 538 3 415 362 87 231 140 1 1592NIO2 4 I 19 379 83 147 602 227 618 32 97 231 Its 14 ) ,
1612N182 6 2 625 386 185 256 165 s 1622N182 6 3 367 M 134 l'.8 235 le 4 3 163ZN182 6 4 425 343 lie 240152 8 6 3 16425182 6 5 25 25 381 93 150 616 248 8 1 165ZNI82 4 4 4 ' 418 373 94 242 161 5 1 166ZNis2 6 7 388 86 154 621 237 4 3 1682N182 4 9 5 '
5 1 169Zuf82 418 11 5 6 170ZNII2 5 9 185 191 124 234 147 4 229 171 150 159 135 2 2 2 1722N182 412 11 270 282 129 167 144 13. 2 174ZN182 ell 19 200128 III 23314 (1 7 l?6ZuiO2 414 8 187 99 121 226 159 le 238192116164153 4 IF 5 I?tzule2 26 164 103 125 241 147 1 1 -
!???N182 414 2 228193 IM 160144 (l i 1: NIO2 417 185 IM 120 229154 19 2 3 1812NTB2 418 341 243 297 185 271 3
' 1822NI82 4tt 8 177 105 119 236 149 (
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R IILUTER A SILUTER 8 IILUTER C I!LUTER 8 N I
- il 46 il 4 E V N E let N C L 184 E C L M N EN MN L C 11 181 L C EN N L EC N M 181 L EN 11 C let L EN N C N lel L N EC A E l 2 3 4 5 6_7 8 f 10 I1 12 13 14 15 ILl? 18 lf 20 21 22 23 24 25 26 27 28 29 38 31 32 33 34 35 34 3L30 39 40 48 42 43 44 45 M 4L48 8 R 2252N182 916 138 383 259 63 26 300 257 125 11 87 150 290 168 195 360 304 le 362 274 200 218178 17 2 4 227N182 917 5 395 273 89 M 144 327 326 137 210 187 9 229ZNI82 918 138 385 267 137 59 43 263 132 170 355 226 4
, 231ZNT82 919 300 278 76 8 105 151 398 284142 It? 158 2 233ZNT81920 124 362 269 145 67 29 379 264 103 9 76 146 249139 let 343 216 8 300 26614 210 les 23 5 2 235ZNf 82 921 397 254 104 84 146 2 307 288 160 209 187 2 237ZNill 922 114 372 262 157 60 265140175 3M 232 23 3 T 'ZNII1923 393 275 88 91 147 311 286 145 216 170 1 2 2 23928T82 924 9 142 385 260 158 61 261 142 163 347 216 286 2 1
~
I4RE B-33 *
PROACI St 10TE ZluC t
SILUTER A filLUTER 3 BItuTER C 4 44 BILUIER 5 N I 46 46 4 E 9 5!-2 E 151 1 4N 5 C&_.7 L lut E C L M N E N lel N L C N lel L C EN N L Et N lut 101 L E N N C ML EN N C N lel L M E C 4 E 8 f !0 !l 12 13 !4 15 I4 17 18 19 20 21 22 23 24 25_26 27 28 29 10 31 3L.33 34 35 36 3LM 39 40 41 42 43 44 M 46 4L48 8 8 2472miO214 7 2F2Wl8210 8 34 95 && 38 24 II et It 28 13 le 42 97 && 19 170 53 45 207 74 21 57 54 4 I I 73 14 33 57 64 2 212mIO216 9 31 84 39 19 250Zule21014 31 78 217 45 2 1 71 24 37 100 57 N 1 251ZNit210ll 24 83 27 "52Zu1821012 M 158 52 I et 16 49 147 42 97 1
?51 3 I821013 21 to 40 29 18 17 g 80 39 22 g g B3 31 2214 M 35 g at le g g j l 1 25ilul82,0l? 42 28 28 162 57 77 20 83 31 16 42 122 38 1 25aZule21018 86 93344 259tul8210lf 24 94 34 171 53 39 4 2402Nia21420 13 5 82 44 59 41 3 21 27 el 52 34 36 20 79 74 15 19 38 47 70934 34 ' 47 48 4147 71 3B 42 7 3. 2 241Zule21421 23 to 45 37 42 43 24 2427mla21422 92 33 42 t
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.a 4 N B.ECT 48 liFLIElli IIE B DILNIER 4 BILNTH 9 BILNB C BILNIS B N I
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PISACT 61 IIFLM ZM B
DENIG A BENTER B BENMR C DEWIER B N I
'N EmN C L MEC L M N EN W5 L C N NL C N N L EC N WWL EN N C ML EN N C N NL N EC
! S 1 4 5 L.7 0_f It lL12 lLl4 15 1L17 18 19 20 21 M Sa M M '728 29 30 31 n n 1L3L3L37 M N 40 41 42 4L44 4L4LO 48 8 8 1513182 $20 St IN 504
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!blEfif 1913182 ell 100132 2B 382 2 102 689 229 H IN 91 114 222 183NIO 620 252 ?! !!4 194N182 621 119 114 212 TAILE B-37 PABACT 71 IINLM ZilE t
BRNIER A BENTER B BENItB C HLBTER B N I Eg sEmN t a s aC L MEC7 g 9 L lg.,33 M N12 EN MNlLILle lLIL15 L ClfN20 WL 21 S' C EN N L EC 5 WWL EN N C WL EN N C N WL 5 EC S 58 M SA '7 25 29 30 31_ n_ _ n is n u 37 38 39 40 45 42 43 44 45 4L4L40 B B 225N182 916 147 gg
! 227N102 917 225 83 241 161 Ul9m 235Zult2 921 n m 219 223 32 F1144 m 18, u
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PRBACT 31 B190EED IIE B
DEUTH A BILUIER B DEUTS C DEUTER B B I H :: lt 4
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"1 ', 5 ". UG_Liz_id_R_UF_LUS.J L L 64 L Ud_LE n 5, L k"n Ud.1.LU2_L:_t_LU. g . l 433 200 1673 5 1&Det2 2 5 2244 912 5 26 deb 2 2 8 2 2915 754 1 5 276 TAKE D-40 PRSICT 41 319IEMB IIE I
BRUTH A BILUTH 3 BILUID C BILUTH B N 1 1
46 .. 44 . E V EN C III N L EN 181 L M C C L 151 E N N E C 101 5 N L C N L lt N 188 E C N L N III L N EN III C L Bl 18. N N C 4 E
_1 S 1 4 1 L.7 8 Lle ll 12 lLl4_15 lLILit lf 20 21 ?? S1 S4 2L2L27 X 2L30_31_12 11 L35_36 37 M N_40 41 42 4L44 45 4L4L40 0 8 NZNB02 222 13 279 6171239 515 ID 263 40 Des 2 225 1200 M2 204 4 4 TAKE B-41 PROKCT St IINEVEB IIE BRUTER A BRUTER 3 BRUTER C DRUID 8 N i it :( 4: : ( y EN C IOf N L EN lel L M C C L III E R N EC let N N L C N L EN III E C N L N 31 L N EN let C L III E N N C e E
_I 1 3 4 5 &._7 I f 10 l1 12 13 14 1LILl7 18 It 20 21 22 23 24 25 2L27 28 29 30 31 12 11 LM 36 3L38 N_40 41 42 43 44 45 4L4L48 9 R
- 83 Des 2 318 523 29 261 12 144 W l Mlle82 324 13134 37 39 32 106 4' 4 ftDes2 15 570 M 130 24 III 52 2 187Z1582 4 7 546 49 156 20 let 54 1 il4 Des 2 414 3 133 32 Sie 13 177 51 121 Des 2 421 22 530 42 144 37 183 51 128Ze 428 l # 02 578 17 200 59 2 13521882 5 5 2 400 34 lie 23 101 49 1 142Z1802 512 143 37 421 22 210 50 l
l
PNBICT 61 BRIIEEEB IIE ,
ORAFT 8 NLNER A DENIB B BILNER C H
BILNEN D E I
- :: :: 4 ( e NI t E 1 4lll9N4 C 7 0Lf 10 MEC tL12L1L14 M N15EN III N Llf 2t_21 1&_1Lle C N WL C $8 SS St EN_S4 N XL ??EC28529 WWL 30 33EN N nCuWL n n_u n_jg_m.,ge_g_q EN N C N 101 N EC e gt a g.,g.,Lq_g_g7_g 1971502 4 1 17 74 205 1762WB2 5 217 IM IN TAKE B-43 FRSICT ?! BISK4EB IIE BRNER A Ie BILETER B BILNEN C BENG B
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N E lti N C L lel E C L N 11 EN 101 N L C N WL C EN N L EC N W III L EN N C EL EN N C U ML 5 EC 4 E
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14EE B-44 PIBICT 8t BlSIEVEBZIK DENIEN A R BRWIG B BILNG t BENIB B N 1 N1_2_3 E lel N C L III E C L M 11 EN WN L C N WL C EN N L EC N III W L EN N t WL EN N C N III L N EC 4 5 & 7 I f lLit 12 lLl4 15 14 1 Lit 19 20_21 SS St M nu '?20 29 30 31 n n 34 n 34 3L3 39 40 41 42 43 44 5 4L4L40 3 0 2:i21N0210 9 BB 37 20 2477158210 7 27 177 as 26 14 29 129 33 83 13 33 ll 3 76 18 2 42 4 143 21 N 93 20 18 1 lH 29 4 19 124 47 4
N25882121 82 15 24 133
$N 25 3 4
FIGURE B-1 PROJECT 3 CbPPER OR ZINC 140- DILUTER A 3200- DILUTER ~
B 120- - -
2800- -
j p 2400-p 100- - : j B - i P 2000- - !
80- -
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FIGURE B-2 I
PROJECT 4 COPPER 110- DILUTER A, 110" DILUTER B 100- -
100- -
, 90- -
90- -
h 80- 80-2 B 70- -
. 70- -
l 60- -
60- -
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m- ~
DRAFT PROJECT 4 ZINC 3000" DILUTER B 000" DILUTER D 2500- -
2500- -
l h2000-2000- -
B 1500- -
1500- -
1000-h 1000- - - -
C _
500- - -
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vz.ouas a-4 PROJECT 5 COPPER OR ZINC 100" DILUTER A 100" DILUTER B l 90- -
90 -
l 80- * = NO- 00 .
! 70- LABILE 7 g. .
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60- -
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10- - * !
OT I f 09 !
l 0 2 4 0 2 4 700" DILUTER C 700" DILUTER D l 600- -
600 -
l l p 500- - --
500- -
P l B 400- -
400- -
Z 300- -
300- -
l I
$ 200- 200-l 100- -
100- - !
Ot 0 2 4
Ot"9 0 2 4 RELATIVE CONCENTRATION OPEN = TOTAL, SHADED = DISSOLVED SOLID.= LABILE
F,IGURE B-5 PROJECT 6A COPPER AHD ZIHC (ACUTE) 110- DILUTER A 110" DILUTER D_
100- -
_ 100- -
C 90- -
90- -
_. 2 p
B 80-70-p 80-B 70-r-
T C
60- -
~'
C 60- -
- E M
0 50- -
0 50- - -
l P 49 -
_ P 40- -
0 h 30- h 30-R 20- -
R 20- - '
@3 10- -
10- -
l 5
l 0? !- ! 0? -!
I' 0 2 4 0 2 4 7
DILUTER A DILUTER D' 200- -
r h 160- _ ,
B - 4 NO ZINC 120- -
i Z '
I -
80' l
N =l C :d 40- -
ll
- a OP*!0 2 4 RELATIVE CONCENTRATION OPEN = TDTAL, SHADED = DISSOLVED SOLID.= LABILE
FIGURE B-6 PROJECT 6 CDPPER AND ZINC (ACUTE) 240;_ DILUTER 0-20 A 2gO_DILUTERD 200-O 200- -
y i P 180- -
180- -
g l
B 160- -
160- -
Ti 140- -
140- -
H C 120- -
120- -
f p
100-80-
- 100-80-l E 60- -
60- -
- l l R 49 .
49 .
l 20- 20- jl 0+l!
g 0+ ! ! "!
0 2 4 0 2 4 DILUTER A DILUTER D 600- -
p 500- - l l
P l B 400- -
NO ZINC l Z 300 .
I L
$ 200- _
i 100- -
I i
l Ot ! !
l 0 2 4 l l RELATIVE CONCENTRATION :
OPEN = TOTAL, SHADED = DISSOLVED SOLID = LABILE l
~ ~ ,
DRAFT PROJECT 6 COPPER (SUBACUTE)
DILUTER B- DILUTER C 60- -
60- -
h 50- -
50- -
B 40- -
40- -
C _- _
0 30- -
30- -
E 20 -
_ 20- -
-) ,
R -
10 -
10- -
0.E 0 2 i
4 0.
0 2 i
4 i
RELATIVE CONCENTRATION OPEN = TOTAL, SHADED = DISSOLVED SOLID = LABILE l
l u --
. FIGURE B-8
- 1 PROJECT 7 COPPER 1'
" DILUTER A DILUTER B 140 -
140- -
P 120- - -
120- -
P .
B 100- -
_ ; 100- -
l
. C 80- -
- i 80- -
0 : :
P 60- - - '
60- -
i P : :
E 40- -
. : : 40- -
1 R i i i 20- -
! ! ! 20- -
0 l ! ! 0 l ! !
- 0 2 4 0 2 4 i
- DILUTER C DILUTER D l 140- -
140- -
l P 120- - .
120- -
P i B 100- -
! 100- -
- ~
( C 80- - -
80- -
0 P 60- -
60- -
l P - -
E 40- -
40- -
R -
a i 20- -
20- -
i 0 l @! ! O O 2 4 0 2 4 RELATIVE CONCENTRATION OPEN = TOTAL, SHADED = DISSOLVED SOLID = LABILE
~
PROJECT 7 COPPER AMD ZIMC DRAFT "
140-DILUTER 140-'D I LUTER B '- D 120- -
120- -
P p 100- - :
100- -
8 -
l 80- -
80- -
1 C
O 50- -
50- -
l_I P -
P 4g. .
- 1 4 g. .
i ,
E q , ,
p' n :
1 .
20- - -
i : 20- -
j -
['!2 1 -
d , :
0 l
~
O l
~
l' O 4 0 2 4 400-DILUTER B_ DILUTER D 350- -
350- - -
300- -
300- -
P ,_
P 250- -
250- -
B 200- - l 900- -
- J I l 2 : -
!I 150- - -
i 150- -
!N l
N 3 t
~
17 =: '
C 100- -
1 i 4
100- -
!?
- n 1
9 #j 4 1 50- -
i, i
50- -
l.l m
ig
.3, rm t a 0m: .
i
,, : i i
O 0
2 i
4 i
0 2 4 RELATIVE CONCENTRATION OPEN = TOTAL, SHADED = DISSOLVED SOLID = LABILE
F'EGURE B-10 PROJECT 8 COPPER 60-h "
60' DILUTER A DILUTER B 50- * = NO 50- -
p LABILE B
40- 40- -
l C 30- -
_ 30- -
O P
l P 20- -
20- -
E R 10- -
g- l 10- -
I O? ! I' O? ! ! l 0 2 4 0 2 4 60-DILUTER C 60" DILUTER D 50- -
50- -
P :
B 40- -
40- -
l C 30- -
30- -
l 0 h 20- - -
20- -
i E l R 10- -
g 10- -
-. L !
0? ! 0? ! !
0 2 4 0 2 4 RELATIVE CONCENTRATION OPEN = TOTAL, SHADED = DISSOLVED SOLID = LABILE
DRAFT FIGURE B-11 PROJECT 8 ZIHC 160' DILUTER B 160" DILUTER D 140- -
140- -
p 120- 120-P 100- -
100- -
B 80- -
80 -
Z -
I 60- -
60- -
N -
C 40- -
40- -
_ g
~
20 -
20- -
.. ]
OY0 2 4
! 0@0 2 4
RELATIVE CONCENTRATION OPEN = TOTAL, SHADED = DISSOLVED SOLID = LABILE l
APPENDIX C WATER CHEMISTRY DATA Table C-1 presents the daily data collected manually by probe in a medium level toxicant tank, the head box of one diluter, and the river. Also presented is data from the nearby USGS quaging station on the Chehalis River.
Table C-2 presents the results of the water chemistry analyses conducted in the chemistry laboratory on water samples.
Table C-3 is a correlation matrix of all of the water cuality data for the 9-day LC50 tests. The data was averaged for each project rather than attempting to correlate on a point by point basis since the LC50s represent a test average value.
Figures C-1 through C-8 graphically present the daily probe ,
measurements in the head box and aquaria.
C-1
p D h (-==]=
TABLE C-1 .i .}
PROJECT 3 TANK HEAD BOX RIVER 1
MMDD A6~~i5P~~56~~P5"~E55 Y5P~~56-~P5~~Ck5 iUE~~56~~P5~~656~~~6E5" 2 5 22 6.0 11.6 74. 5.5 10.1 72. 5.1 11.4 10900 2 6 25 5.5 11.3 68. 4.8 11.8 69. 4.9 11.8 69. 9700 2 7 35 5.0 12.3 66. 4.1 12.4 66. 8680 l 28 5 4.3 12.5 65. 3.5 12.2 90. 7890 2 9 18 4.8 12.7 74. 4.0 12.5 65. 7230 l 210 15 4.0 12.2 70. 3.2 12.6 66. 6640 211 10 5.0 12.0 7.1 64. 4.3 12.2 66. 3.8 12.7 68. 6500 212 5 5.5 12.4 66. 5.0 68. 4.7 12.5 69. 74800 213 5 6.6 12.2 50. 23100 214 5 7.2 11.6 49. 41300 l
l l l
PROJECT 4 TANK HEAD BOX RIVER MMDD A6~~i5P PA~~C56 55P~~56~~PU~~CIE 75P-~56- eR- 6A5-- 6Fi-222 30 7.3 9.9 6.7 53. 7.0 10.6 6.9 73. 37500 223 24 6.5 10.6 6.8 60. 6.1 10.5 4.7 57. 5.9 11.0 6.6 57. 33800
, 224 26 6.0 11.0 6.7 62. 5.8 10.8 6.8 58. 5.3 11.0 6.9 61. 26900 225 11 6.3 11.4 7.0 60. 6 1 11.2 6.8 57. 5.3 11.4 58. 20400 226 18 6.1 11.4 6.7 43. 6.5 11.2 6.7 60. 6.3 11.2 6.7 60. 17300 227 7 6.8 11.0 6.7 61. 6 6 11.2 6.8 61. 6.0 11.3 6.9 16700
. 228 22 7.0 11.4 6.7 64.- 6.5 12.0 6.7 61. 6.0 11.4 6.8 61. 15600 3 1 48 7.1 10.5 6.7 53. 7.0 10.9 6.8 53. 7.0 11.0 6.8 53. 17400 3 2 11 7.0 11.1 6.6 64. 6.8 10.8 6.6 55. 6.8 11.0 6.9 57. 18600 3 3 38 7.4 11.0 6.6 61. 7.2 10.6 6.9 53. 7.0 10.8 6.7 52. 19800 3 4 46 7.6 11.1 6.5 62. 7.6 11.1 6.9 53. 7.2 11.2 7.0 53. 21400 i
3 5 38 7.2 11.2 6.6 61. 7.0 11.2 7.1 53. 6.9 11.2 7.1 53. 20000
- 3 6 48 7.8 10.7 4.8 56. 7.2 11.2 7.0 57. 7.0 11.2 7.0 57. 17400 3 7 38 7.2 11.0 6.6 69. 7.0 10.9 6.9 61. 6.6 10.0 6.5 62. 14500 3 8 38 7.3'11.1 6 6 69. 7.0 11.0 7.0 60. 6.9 11.0 7.0 61. 12500 3 9 38 8.1 10.7 6.6 70. 8.0 10.6 7.0 60. 7.8 10.6 7.0 60. 12600 j 310 38 7.3 10.4 4.5 73. 7.1 10.2 6.9 69. 7.0 67. 13600 PROJECT 7 TANK HEAD BOX RIVER I MMDD 56~~iEP~~66'~PA~~6A5 iEP~~56-~PE~~656 TEP~~56~~P5-~6 5~~~CF5" l
915 16 15.2 7.5 7.1 98. 15.8 9.1 7.2 95. 16.1 9.2 7.2 98. 883 917 12 15.2 8.2 7.2 97. 15.0 8.8 7.1100. 15.1 8.9 7.2 775 l 918 16 15.6 9.2 7.1 95. 15.5 8.7 7.3 93. 15.5 8.7 7.2 95. 739 l 919 24 16.0 8.3 7.2 92. 16.0 8.7 7.3 91. 16.0 8.8 7.1 99. 739 920 29 15.3 7.3 7.2 89, 15.1 8.7 7.3 90. 15.1 8.8 7.3 92. 730 i 921 34 15.2 8.7 7.2 90. 15.3 9.3 7.3 87. 15.3 9.2 7.3 739 l 922 41 15.2 8.4 7.2 93. 15.3 9.2 7.2 91. 15.3 9.3 7.0 739 923 46 15.5 8.7 7.0 91. 15.2 8.8 7.1 91. 15 1 8.9 7.1 739 t
TABLE C-1 (CONTINUED)
PROJECT 5 TANK HEAD BOX RIVER MMDB U6~~T5P~~56-~P5~~655 T5P~~56~~PE~~C55 T5P-~56-~P5~~655~~~CE5' 318 5 8.1 8.7 6.9 68. 8.1 11.0 7.2 69. 7.3 11.0 7.1 69. 11000 319 11 7.8 10.5 6.9 68. 7.7 11.0 7.1 68. 7.5 11.0 7.1 69. 9600 320 29 7.2 10.0 7.0 70. 7.2 10.9 7.1 70. 7.0 10.9 7.1 70. 8420 321 20 7.1 10.2 7.1 70. 7.0 10.9 7.1 70. 6.9 10.9 7.1 70. 7760 322 26 8.2 10.1 6.9 71. 8 5 10.8 7.2 70. 8.5 10.8 7.2 70. 7120 323 34 8.0 10.2 7.0 71. 7 9 10.9 7.1 71. 7.7 10.9 7.1 71. 6600 324 38 9.5 9.5 4.8 76. 9.6 11.0 7.1 73. 9.1 11.1 7.1 73. 6200 325 46 9.3 10.6 7.1 78. 8.8 11.0 7.0 78. 8.5 11.0 7.2 74. 5860 326 5 9.9 10.7 7.0 79. 9.3 10.8 7.1 78. 9.3 11.0 7.0 79. 5620 327 11 9.1 10.5 7.0 70. 9.0 10.8 7.1 70. 8.6 10.8 7.1 70. 5660 328 18 9.5 10.5 6.9 69. 9.3 10.9 7 1 70. 9.1 10.9 7.1 71. 5740 329 22 8.7 10.2 6.9 71. 8.8 10.8 7.1 71. 8.3 10.8 7.1 71. 5580 330 26 8.3 10.6 6.8 72. 8.6 10.8 7.1 70. 8.8 10.8 7.1 70. 5740 331 35 8.2 10.8 7.0 70. 6.0 10.8 7.2 70. 7.7 11.0 7.1 70. 5980 4 1 38 8.0- 9.7 7.0 71. 7.9 11.0 7.0 71. 7.4 11.0 7.2 6410 4 2 46 7.9 11.2 7.2 71. 7.6 11.2 7.1 71. 7.0 11.2 7.1 6930 43 5 7.1 11.4 7.0 69. 7.1 11.3 7.1 67. 6.6 11.4 7.3 9520 4 4 11 7.1 11.4 6.9 69. 6.9 11.5 7.1 65. 6.7 11.5 7.1 12700 4 5 18 7.2 11.4 6.9 70. 7.0 11.8 6.9 66. 6.8 11.8 7.0 66. 12100 4 6 22 7.9 11.8 6.9 70. 7.2 12.0 7.0 66. 7.7 12.1 69. 10700 4 7 26 8.0 10.6 6.9 70. 8.0 10.7 7.0 69. 7.7 10.8 7.2 9780 4 8 35 8.4 10.8 6.9 71. 8.5 11.0 68. 8.3 11.0 70. 8780 4 9 38 8.8 9.2 6.9 78. 8.8 10.8 7.1 69. 8.5 11.0 7.1 69. 7600 410 38 9.0 9.2 73. 9.0 10.6 7.1 70. 8.9 10.7 7.2 7090 411 38 9.9 8.9 7.0 70. 9.8 10.2 7.3 68. 9.5 10.5 7.2 7950 412 46 8.8 10.5 7.0 64. 8.5 10.6 7.1 62. 8.2 10.8 7.2 12600 413 47 8.7 9.9 7.0 62. 8.3 10.8 7.2 60. 8.0 10.8 7.1 65. 16100 414 8 8.0 9.9 61. 7.5 11.1 61. 7.0 11.1 17800 415 24 7.4 10.2 7.0 7.2 11.2 7.0 61. 7.4 11.3 7.1 18900
-* 416 17 7.5 10.4 6.9 69. 7.1 11.0 7.0 63. 6.9 11.1 17900 417 29 8.0 10.2 6.9 65. 7.9 11.4 7.0 64. 7.8 11.5 7.1 16000 418 33 8.0 10.7 6.9 46. 8.0 11.2 7.1 65. 7.6 11.4 7.1 13800 419 40 8.0 10.0 7.0 68. 7.8 11.1 7.1 68. 7.6 11.2 7.1 11700 420 8 8.8 19.0 7.0 69. 8.6 10.8 7.0 69. 8.5 11.0 7.1 68. 10100 421 17 9.8 9.4 6.9 71. 9.8 11.0 7.1 68. 9.5 11.1 7.1 67. 8800 422 23 10.7 8.8 6.8 70. 10.7 10.6 7.1 69. 10.5 10.6 7.1 8010 423 29 11.5 7.9 7.0 72. 11.3 10.2 7.1 69. 11.2 10.3 7.2 735C 424 33 11 9 9.1 7.0 71. 12.0 10.6 7.1 70. 11.8 10.8 7.0 6690 425 40 11.0 8.7 7.0 71. 11.2 10.6 7.1 70. 11.0 10.7 7.1 6140 426 47 11.0 8.9 7.0 73. 10.9 10.5 7.0 73. 10.5 10.7 7.1 71. 5600 427 2 10.7 8.8 7.0 73. 10 4 10.0 7.0 69. 10.2 10.1 7.0 69. 5240 428 8 11.1 7.9 7.0 75. 11.4 9.6 7.2 71. 11.1 9.4 7.2 69. 5420 429 17 10.7 8.8 6.9 76. 10.5 10.4 7.2 71. 10.1 10.6 7.1 5500 430 23 10.8 8.6 6.9 77. 10.6 10.6 7.2 75. 10.5 10.6 7.2 73. 5060 5 1 29 11.0 8.3 7.0 74. 10.9 10.4 7.1 74. 10.7 10.5 7.2 75. 4580 5 2 33 11.3 9.0 7.0 76. 11.2 10.2 7.2 75. 11.0 10.2 7.2 76. 4270 5 3 40 11.0 8.7 7.0 77. 11.1 10.8 7.2 75. 11.0 10.8 7.2 4230 5 4 47 11.6 9.3 7.0 76. 11.9 10.9 7.1 74. 11.8 11.0 7.1 4050 55 2 11.6 8.9 7.2 77. 12.0 10.8 7.3 75. 12.0 10.8 3810 56 8 12.3 8.4 6.9 76. 12.2 10.3 7.1 75. 12.3 10.5 7.2 3540 5 7 17 12.9 8.4 7.0 82. 12.6 10.2 7.2 83. 12.3 10.3 7.2 3390 5 8 23 11.8 8.6 7.0 76. 11.7 10.6 7.2 78. 11.2 10.7 7.2 3250 5 9 29 11.3 8.2 7.0 77. 11.2 10.4 7.2 76. 11.0 10.4 7.2 3120 510 33 11.9 9.2 7.1 79. 12.0 10.6 7.2 75. 11.8 10.7 7.3 2990 511 40 12.0 7.9 7.1 78. 11.7 10.2 7.2 76. 11.5 10.3 7.3 2850 512 47 12.0 8.6 7.0 81. 12.2 10.4 7.2 77. 12.2 10.5 7.1 2760 513 2 12.0 8 6 7.0 80. 11.9 10.7 7.2 77. 11.5 10.6 7.0 2660 l 514 8 12.1 8.6 7.1 78. 12 2 10.6 7.2 76. 12.0 10.9 2620 515 17 12.0 8.1 7.0 78. 12.1 10.6 7.2 77. 12.0 10.7 7.2 2520 516 23 13.7 7.5 7.0 81. 13.7 10.3 7.2 81. 13.2 10.4 7.3 2490 517 29 13.5 6.9 4.9 82 13.3 10.1 7.2 79. 13.1 10.2 7.3 2420 518 33 13.2 9.4 7.1 79. 13.0 11.0 7.2 78. 12.9 11.4 7.2 2350
TABLE C-1 (CONTINUED)' , f y" h ?*)}
{/ Y PROJECT 6 ~" "" OL $ '
~
TANK HEAD BOX RIVER i MMDD U6~~T5E~~56~~EU~~E 5 T5E~~56~~E5~~C05 T5E~~56~~E5~~C55~~~6E5" 528 1 14.0 9.6 6.9 81. 13.9 10.0 7.1 81. 13.8 10.1 7.3 1840 529 12 15.8 9.8 7.2 84. 16.2 10.8 7.4 83. 16.2 10.Y 7.4 1810 530 16 15.5 9.3 7.2 82. 15.2 9.6 7.1 83. 15.0 9.7 7.3 1720 531 24 16.2 9.3 7.2 82. 16.2 10.0 7.1 82. 16.2 10.1 7.3 1660 6 1 29 15.8 9.8 7.4 83. 15.6 10.4 7.5 82. 15.5 10.5 7.3 1600 6 2 34 14.6 9.8 7.3 82. 14.5 10.4 7.4 82. 14.4 10.7 7.4 1590 6 3 41 14.4 10.4 7.4 85. 14.5 10.8 7.5 82. 14.3 10.8 1600 6 4 46 14.3 10.0 7.3 85. 14.3 10.3 7.4 84. 14.0 10.4 7.5 1590 65 1 14.4 10.0 7.1 85. 14.4 10.4'7.4 84. 14.1 10.5 7.5 1590 6 6 12 14.1 10.0 6.9 83. 14.2 10.7 7.2 82. 14.1 10.8 7.3 1550
. 4 7 16 14.3 9.6 7.0 85. 14.2 10.6 7.3 81 13.6 10.1 6.9 1530 i 6 8 24 14.7 10.0 7.2 84. 14.6 10.6 7.4 83. 14.2 10.8 7.1 1460 '
6 9 29 14.4 9.7 7.3 85. 15.3 10.4 7.4 82. 15.1 10.6 7.3 1410 610 34 14.5 9.8 7.1 85. 16 4 10.1 7.2 84. 17.0 11.0 7.5 1340 611 1 17.0 9.6 7.1 86. 17.1 10.2 7.4 84. 16.8 10.3 7.4 1290 612 12 16.7 8.7 7.1 87. 16.2 9.8 7.1 87. 16.0 9.8 7.2 1260 613 16 16.3 9.6 7.2 87. 15.2 10.4 7.4 89. 15.0 10.6 7.5 1230 614 24 14.8 10.0 7.2 84. 14.9 11.8 7.7 83. 14.8 12.2 7.8 1740 615 29 15.3 9.6 7.4 83. 15.1 10.5 7.3 83. 14.8 10.9 7.5 1240 616 34 16.3 9.6 7.4 84. 16.5 10.8 7.8 84. 16.4 11.2 7.9 1190 617 41 17.8 9.5 7.3 86. 17.6 10.2 7.3 86. 17.3 10.3 7.5 1170 618 46 18.9 9.3 6.9 87. 18.8 9.8 6.9 87. 18.8 10.0 6.9 86. 1160 619 1 19.4 8.4 7.1 87. 19.7 9.0 7.3 87. 19.2 9.0 7.2 1140 620 12 19.3 8.0 7.1 88. 19.2 9.1 7.2 87. 18.9 9.0 7.3 1140 621 14 19.1 8.5 7.2 89. 18.9 9.2 7.4 86. 18.5 9.8 7.4 1120 622 24 18.1 9.0 7.3 86. 18.3 10.4 7.7 86. 18.2 10.6 7.9 1120 623 29 18.2 8.0 7.3 86. 18.3 9.2 7.5 86. 18.1 9.6 7.6 1100 624 34 18.8 8.5 7.3 87. 18.9 9.2 7.5 87. 18.6 9.9 7.7 1080 625 16 19.0 8.2 7.2 86. 18.9 9.0 7.3 85. 18.8 9.1 7.5 1080 626 24 18.0 8.6 7.2 82. 17.9 9.1 7.3 80. 17.7 9.2 7.3 1280 627 29 17.3 9.0 7.3 79. 17.2 9.8 7.4 79. 17.0 9.9 7.5 1510 628 34 17.0 8.9 7.1 82. 17.0 9.5 7.2 79. 16.8 9.5 7.5 1390 629 16 17.5 8.8 7.3 87. 17.5 9.1 7.4 86. 17.2 9.1 7.4 1340 1260 630 24 17.8 8.6 7.4 86. 17.5 9.0 7.4 86. 17.3 9.1 7.4 7 1 29 17.0 8.6 7.3 87. 16.9 9.2 7.4 87. 16.6 10.3 7.5 1230 7 2 29 16.7 9.3 7.2 89. 16.3 9.9 7.2 88. 16.3 9.9 7.2 1220 7 3 34 16.1 8.8 7.1 87. 15.9 9.4 7.1 88. 15.8 9.5 7.3 1200 7 4 16 15.6 8.8 7.0 83. 15.2 9.2 7.2 84. 15.2 9.3 7.1 1180 7 6 24 15.9 8.5 7.1 84. 15.8 8.9 7.1 84. 15.5 9.0 7.1 1160 7 6 29 16.0 9.4 7.2 04. 16.3 9.9 7.0 82. 16.1 10.0 7.2 1140 7 7 34 17.0 8.5 7.1 87. 17.0 9.0 7.1 85. 17.1 9.4 7.2 1120 7 8 16 17.2 8.5 7.2 88. 16.8 8.8 7.2 87. 16.8 9.0 7.3 1100 1070 7 9 24 17.9 8.6 7.1 89. 17.9 8.8 7.0 88. 17.7 8.9 7.3 710 29 18.1 8.0 7.0 88. 18.1 8.2 7.0 89. 17.8 8.7 7.2 1040 711 34 18.8 8.3 7.1 88. 18.8 8.7 7.1 88 18.7 8.8 7.2 1000 712 16 18.2 8.7 7.4 88. 18.3 9 0 7.4 88. 18.0 8.8 9.2 7.3 88. 973 713 24 19.0 8.2 7.1 87. 18.8 8.8 7.1 87. 18.4 7.3 955 713 29 17.0 8.1 7.3 87. 17.2 8.4 7.3 87. 17.0 8.6 7.5 982 715 34 16.8 8.5 7.1 86. 16.6 9.0 7.2 85. 16.2 941 7.2 964 )
716 16 16.3 8.6 7.2 86. 16.3 9.0 7.3 85. 16.1 9.1 7.4 973 717 24 17.5 8.7 7.1 88. 17.4 9.3 7.2 87, 17.1 9.0 9.3 7.2 955 718 29 18.2 8.4 7.2 88. 18.1 9.0 7.3 88. 17.9 9.0 7.2 7.3 928 901 719 34 17.2 8.5 7.2 89. 17.1 9.0 7.2 89. 17.0 720 16 17.7 8.0 7.2 91. 17.6 8.4 7.2 90. 17.5 8.5 7.2 983 721 24 17.1 8.9 7.2 89. 17.1 9.3 7.3 89. 16.1 9.4 7.3 865 722 29 17.5 8.8 7.3 90. 17.9 9.4 7.3 88. 17.6 9.4 7.4 838 723 36 17.4 8.6 7.3 88. 17.3 9.2 7.3 88. 17.1 9.2 7.4 829 724 16 18.1 8.6 7.2 88. 18.0 9.0 7.3 88. 17.8 9.0 7.3 802 725 24 18.7 8.2 7.2 89. 18.6' 8.6 7.2 90. 18.2 8.7 7.2 793 726 29 18.3 8.1 7.1 89. 18.7 8.6 7.1 87. 18.6 8.6 7.1 775 727 34 18.8 8.2 7.0 88, 18.8 9.0 7.1 87. 18.4 9.1 7.1 757 728 16 19.0 8.2 7.1 87. 18.8 9.3 7.5 86. 18.5 9.3 7.6 87. 748
TABLE C-1 (CONTINUED) ,
PROJECT 8 ,' .e '..] 1 TANK HEAD BOX RIVER nnDD H5- fiF Fi- Eis fRF Fi- EE5 iRF-~55- Fi- Eii-- Efi-10 7 1 12.8 8.2 7.2 91. 11.9 10.4 7.4 87. 11.8 10.4 7.4 1060 10 8 12 12.1 8.9 7.1 87. 11.6 10.0 7.3 86. 11.5 10.1 7.3 1170 10 9 16 12.3 7.3 7.2 88. 12.4 9.3 7.3 87. 12.0 9.5 7.3 1300 1010 24 12.1 8.9 7.1 89. 11.8 9.7 7.2 93. 11.4 9.7 7.2 1170 1011 29 12.8 7.8 7.1 93. 12.5 10.4 7.2 90. 12.5 10.4 7.3 1060 1012 34 12.7 8.6 7.2 94. 12.1 9.6 7.4 92. 12.0 9.7 7.4 973 1013 41 12.7 7.6 6.9 95. 12.2 10.0 7.0 93. 12.1 10.1 7.2 901 1014 46 13.0 8.8 6.9 93. 12.7 10.4 7.2 89. 12.9 10.4 7.2 847 1015 46 12'.2 9.0 6.9 92. 12.2 9.6 7.3 93. 12.5 9.6 7.3 811 1016 12 12.9 8.6 7.1 93. 12.8 9.8 7.1 91. 12.3 9.8 7.1 793 1017 16 11.9 7.3 6.9 90. 11 3 9.2 7.2 89. 11.1 9.3 7.1 883 1018 24 10.9 9.8 7.0 88. 10.6 10.6 7.2 88. 10.3 10.7 7.3 910 1019 29 10.0 8.5 7.2 90. 9.3 10.1 7.2 88. 9.3 10.2 7.1 910 1020 34 9.7 9.5 7.0 90. 9 2 10.6 7.1 89. 9.1 10.8 7.3 901 1021 41 10.0 8.4 7.0 92. 9.9 10.6 7.1 88. 9.7 10.6 7.1 910 1022 46 10.5 9.4 7.1 81. 10.0 10.6 7.1 77. 9.9 10.6 7.1 3270 1023 1 10.7 8.5 6.7 70. 10.3 10.4 7.1 69. 10.2 10.4 7.0 3920 1024 12 11.3 8.8 7.0 84. 11.1 9.9 7.2 82. 11.0 10.0 7.3 3020 <
1025 16 11.0 7.8 6.9 85. 10.6 10.1 7.0 82. 10.4 10.2 7.3 2420 1026 24 11 0 9.2 7.0 78. 11.0 9.2 7.6 77. 10.5 10.3 7.4 2410 1027 29 10.1 9.2 6.9 71. 9.9 10.8 4.9 70. 9.8 10.8 7.0 3330 1028 34 10.5 9.0 6.9 79. 10.1 10.1 7.1 78. 10.0 10.1 7.1 3650 1029 41 9.8 8.4 6.7 73. 9.2 10.2 6.8 70. 9.1 10.3 6.9 9050 1030 46 9.8 9.4 6.8 77. 9.3 10.3 6.9 76. 9.2 10.4 6.9 10860 1031 1 10.1 8.2 6.7 84. 9.9 10.2 7,0 81. 9.8 10.3 7.0 7260 11 1 12 10.0 9.3 6.8 84. 9.6 10.5 7.0 81. 9.4 10.6 7.1 5260 11 2 16 9.1 8.2 6.5 84. 8.8 10.6 6.6 82. 8.5 10.6 6.6 4210 11 3 24 9.3 9.4 6.7 87. 9.0 10.7 6.9 82. 8.9 10.7 6.7 3820 11 4 29 9.8 7.5 6.7 83. 9.1 10.0 6.8 81. 9.3 10.1 6.9 3780 11 5 34 9.5 9.3 6.7 78. 9.0 10.2 6.5 89. 9.0 10.2 6.9 5310 11 6 41 8.8 8.9 6.7 69. 8.5 10.4 6.7 69. 8.2 10.5 6.6 7460 11 7 46 8.7 9.8 6.9 78. 8.4 10.6 6.9 78. B.2 10.8 6.8 7620 11 8 1 7.8 9.0 6.6 78. 7.2 11.2 6.8 76. 7.1 11.2 6.8 7040 5680 11 9 12 7.0 10.2 6.6 78. 6.5 11.0 6 8 76. 6.3 11.1 6.6 1110 16 6.3 9.3 6.8 81. 6.1 11.6 6 8 77. 5.9 11 6 6.9 4640 1111 24 6.0 11.1 6.9 79. 5.6 11.8 7.0 76. 5.3 11.8 6.7 3870 1112 29 6.1 9.8 7.0 82. 5.7 11.6 7.3 79. 5.4 11.6 7.3 3460 1113 34 6.0 10.8 6.8 80. 5.8 11.5 4.8 83. 5.6 11.6 6.8 3100 1114 41 5.3 10.2 6.9 80. 5.1 11.8 7.0 81. 5.0 11.7 7.0 2820 1115 46 5.7 11.0 6.7 83. 5.1 12.0 6.8 80. 5.0 12.0 6.9 2620 1116 1 6.2 9.3 6.6 82. 6.0 11.2 6.6 77. 5.9 11.2 6.8 76. 3720 1117 12 7.5 10.1 6.7 69. 7.1 11 0 6.7 68. 7.0 11.0 6.7 68. 8220 1118 16 7.7 9.6 6.8 78. 7.1 11.6 7.0 79. 7.0 11.6 7.0 11400 1119 24 8.2 9.8 6.9 72. 8.1 10.7 7.0 72. 7.8 10.7 7.1 11400 1120 29 7.8 8.6 6.8 71. 7.5 10.2 6.9 73. 7.2 10.4 7.0 10800 1120 34 7.1 10.4 6.7 73. 6.9 11 1 6.7 71. 6.5 11.1 6.7 9900 1122 41 6.3 9.3 6.8 75. 6.0 10.8 6.9 73. 5.7 10.6 6.9 8300 1123 46 5.5 11.6 6.7 73. 4.9 12.4 6.7 72. 4.8 12.4 6.8 6960 1124 1 5.0 10.4 6.7 75. 4.5 12.0 6.9 73. 4.5 12.1 6.9 5840 1125 12 4.7 11.2 6.7 74. 4.2 11.8 6.9 74. 3.9 11.8 6.9 5020 1126 16 5.0 10.0 6.8 77. 4.8 11.3 6.9 77. 4.5 11.3 6.9 4480 1127 24 5.8 11.2 7 0 76. 5.3 11.8 7.1 75. 5.2 11.8 7.1 4880 1128 29 7.0 8 9 6.7 69. 6 8 10.9 6.8 69. 6.6 11.0 6.9 7930 1129 34 7.3 10.1 6.7 72. 7.0 10.9 6.8 69. 6.8 10.9 6.8 69. 10800 1130 41 7.7 8.8 6.8 70. 7.5 11.2 7.0 70. 7.1 11.0 7.1 14200 12 1 46 7.8 10.4 6.8 69. 7.2 11.0 6.9 68. 7.1 11.1 6.9 15200 12 2 1 7.7 9.1 6.8 77. 7.1 11 4 7.0 76. 7.0 11.5 7.0 14500 12 3 12 8.5 10.0 6.8 72. 8.1 11.1 6.9 60. 8.0 11.2 6.9 26400 12 4 16 8.3 8.0 6.6 60. 8.0 10.2 6.8 60. 7.8 10.2 6.9 36700 12 5 24 8.0 9.1 6.5 61. 7.8 10.4 6.7 61. 7.5 10.5 6.7 29600 12 6 29 7.5 7.9 6.5 63. 7.1 10.4 6.7 66. 7.0 10.5 4.7 29200 12 7 29 70 8.5 6.4 66. 6.8 10.0 6.5 65. 6.8 10.0 66. 25400 OK,
TABLE C-1 (CONTINUED) y 7.f;h ,
l
=
c .t ,
. PROJECT 6 ' "' a TANK HEAD BOX RIVER HMDD 6"~ TEE ~~56~~EU~~C E T5P es- Ess Tse - 55- R- P ER5-- Eis-528 1 14.0 9.6 6.9 81. 13.9 10.0 7.1 81. 13.8 10.1 7.3 1840 529 12 15.8 9.8 7.2 84. 16.2 10.8 7.4 83. 16.2 10.9 7.4 1810 530 16 15.5 9.3 7.2 82. 15.2 9.6 7.1 83. 15.0 9.7 7.3 1720 4
' 531 24 16.2 9.3 7.2 82. 16.2 10.0 7.1 82. 16.2 10.1 7.3 1660 6 1 29 15.8 9.8 7.4 83. 15.6 10.4 7.5 82. 15.5 10.5 7.3 1600 6 2 34 14.6 9.8 7.3 82. 14.5 10.4 7.4 82. 14.4 10.7 7.4 1590 6 3 41 14.4 10.4 7.4 85. 14.5 10.8 7.5 82. 14.3 10.8 1600 6 4 46 14.3 10.0 7.3 85. 14.3 10.3 7.4 84. 14.0 10.4 7.5 1590 65 1 14.4 10.0 7.1 85. 14.4 10.4 7.4 84. 14.1 10.5 7.5 1590 6 6 12 14.1 10.0 6.9 83. 14.2 10.7 7.2 82. 14.1 10.8 7.3 1550 6 7 16 14.3 9.6 7.0 85. 14.2 10.6 7.3 81. 13.6 10.1 6.9 1530 l
6 8 24 14.7 10.0 7.2 84. 14.6 10.6 7.4 83. 14.2 10.8 7.1 1460 6 9 29 14.4 9.7 7.3 85. 15.3 10.4 7.4 82. 15.1 10.6 7.3 1410 i 610 34 16.5 9.8 7.1 85. 16.4 10.1 7.2 84. 17.0 11.0 7.5 1340 611 1 17.0 9.6 7 1 86. 17.1 10.2 7.4 84. 16.8 10.3 7.4 1290 612 12 16.7 8.7 7.1 87. 16.2 9.8 7.1 87. 16.0 9.8 7.2 1260 613 16 16.3 9.6 7.2 87. 15.2 10.4 7.4 89. 15.0 10.6 7.5 1230 614 24 14.8 10.0 7.2 84. 14.9 11.8 7.7 83. 14.8 12.2 7.8 1240 615 29 15.3 9.6 7.4 83. 15.1 10.5 7.3 83. 14.8 10.9 7.5 1240 i 616 34 16.3 9.6 7.4 84. 16.5 10.8 7.8 84. 16.4 11.2 7.9 1190 617 41 17.8 9.5 7.3 86. 17.6 10.2 7.3 86. 17.3 10.3 7.5 1170 618 46 18.9 9.3 6.9 87. 18.8 9.8 6.9 87. 18.8 10.0 6.9 86. 1160 619 1 19.6 8.4 7.1 87. 19.7 9.0 7.3 87. 19.2 9.0 7 2 1140 620 12 19.3 8.0 7.1 88. 19.2 9.1 7.2 87. 18.9 9.0 7.3 1140 621 16 19.1 8.5 7.2 89. 18.9 9.2 7.4 86. 18.5 9.8 7.4 1120 622 24 18.1 9.0 7.3 86. 18.3 10.4 7.7 86. 18.2 10.6 7.9 1120 l
623 29 18.2 8.0 7.3 86. 18.3 9.2 7.5 86. 18.1 9.6 7.6 1100 f 624 34 18.8 8.5 7.3 87. 18.9 9.2 7.5 87. 18.6 9.9 7.7 1080 625 16 19.0 8.2 7.2 86. 18.9 9.0 7.3 85. 18.8 9.1 7.5 1080 626 24 18.0 8.4 7.2 82. 17.9 9.1 7.3 80. 17.7 9.2 7.3 1280 l 627 29 17.3 9.0 7.3 79. 17.2 9.8 7.4 79. 17.0 9.9 7.5 1510 628 34 17.0 8.9 7.1 82. 17.0 9.5.7.2 79. 16.8 9.5 7.5 1390 629 16 17.5 8.8 7.3 87. 17.5 9.1 7.4 86. 17.2 9.1 7.4 1340 630 24 17.8 8.6 7.4 86. 17.5 9.0 7.4 86. 17.3 9.1 7.4 1260 7 1 29 17.0 8.6 7.3 87. 16.9 9.2 7.4 87. 16.6 10.3 7.5 1230 7 2 29 16.7 9.3 7.2 89. 16.3 9.9 7.2 88. 16.3 9.9 7.2 1220 7 3 34 16.1 8.8 7.1 87. 15.9 9.4 7.1 88. 15.8 9.5 7.3 1200 7 4 16 15.6 8.8 7.0 83. 15.2 9.2 7.2 84. 15.2 9.3 7.1 1180 1 7 6 24 15.9 8.5 7.1 84. 15.8 8.9 7.1 84. 15.5 9.0 7.1 1160 7 6 29 16.0 9.4 7.2 84. 16.3 9.9 7.0 82. 16.1 10.0 7.2 1140 7 7 34 17.0 8.5 7.1 87. 17.0 9.0 7.1 85. 17.1 9.4 7.2 1120 7 8 16 17.2 8.5 7.2 88. 16.8 8.8 7.2 87. 16.8 9.0 7.3 1100 7 9 24 17.9 8.6 7.1 89. 17.9 8.8 7.0 88. 17.7 8.9 7.3 1070 710 29 18.1 8.0 7.0 88. 18.1 8.2 7.0 89. 17.8 8.7 7.2 1040 711 34 18.8 8.3 7.1 88. 18.8 8.7 7.1 88. 18.7 8.8 7.2 1000 l 712 16 18.2 8.7 7.4 88. 18.3 9.0 7.4 88. 18.0 9.2 7.3 88. 973
. 713 24 19.0 8.2 7.1 87. 18.8 8.8 7.1 87. 18.4 8.8 7.3 955 713 29 17.0 8.1 7.3 87. 17.2 8.4 7.3 87. 17.0 8.6 7.5 982 715 34 16.8 8.5 7.1 86. 14.6 9.0 7.2 85. 16.2 9.1 7.2 964 1 716 16 16.3 8.6 7.2 86. 16.3 9.0 7.3 85. 16.1 9.1 7.4 ?73 717 24 17.5 8.7 7.1 88. 17.4 9.3 7.2 87. 17.1 9.3 7.2 955 718 29 18.2 8.4 7.2 88. 18.1 9.0 7.3 88. 17.9 9.0 7.2 928 719 34 17.2 8.5 7.2 89. 17.1 9.0 7.2 89. 17.0 9.0 7,3 901 ]
720 16 17.7 8.0 7.2 91. 17.6 8.4 7.2 90. 17.5 8.5 7.2 983 j 721 24 17.1 8.9 7.2 89. 17.1 9.3 7.3 89. 16.1 9.4 7.3 865 722 29 17.5 8.8 7.3 90. 17.9 9.4 7.3 88. 17.6 9.4 7.4 838 723 36 17.4 8.6 7.3 88. 17.3 9.2 7.3 88. 17.1 9.2 7.4 829 724 16 18.1 725 24 18.7 8.6 7.2 88. 18.0*
3.2 7.2 89. 18.6 8.6 7.2 90. 18.2 8.7 7.2 9.0 7.3 88. 17.8 9.0 7.3 802 793 l
726 29 18.3 8.1 7.1 89. 18.7 8.6 7.1 87. 18.6 8.6 7.1 775 727 34 18.8 8.2 7.0 88. 18.8 9.0 7.1 87. 18.4 9.1 7.1 757 l 728 16 19.0 8.2 7.1 87. 18.8 9.3 7.5 86. 18.5 9.3 7.6 87. 748 l
l
---,-~--v-w - e-em-- - - ' ~ ' ' ' ' ' " " -~
e,e n-.wn --.- ,---- - - - , - - --,, - --- , , - - - - --,--,,-n , , , , , , - - - - - , - - - , _ m-aw-,.m-- >- -w- ,,,,~w.e-,-rn
TABLE C-1 (CONTINUED) l
- PROJECT 8 yq TANK HEAD BOX RIVER
~~
MMDD 6~~TEEI~56~~P C 6 i5P~~66~~PU~~CU6 i P~~56~~P5~~C 6~~~Cf5~
10 7 1 12.8 8.2 7.2 91. 11 9 10.4 7.4 87. 11.8 10.4 7.4 10 8 12 12.1 1060 8.9 7.1 87. 11.6 10.0 7.3 86. 11.5 10.1 7.3 - 1170 10 9 16 12.3 7.3 7.2 88. 12.4 9.3 7.3 87. 12.0 9.5 7.3 1300
. 1010 24 12.1 8.9 7.1 89. 11.8 9.7 7.2 93. 11.4 9.7 7.2 1170 1011 29 12.8 7.8 7.1 93. 12.5 10.4 7.2 90. 12.5 10.4 7.3 1060 1012 34 12.7 8.6 7.2 94. 12.1 9.6 7.4 92. 12.0 9.7 7.4 973 1013 41 12.7 7.6 6.9 95. 12 2 10.0 7.0 93. 12.1,10 1 7.2 901 1014 46 13.0 8.8 4.9 93. 12.7 10.4 7.2 89. 12.9 10.4 7,2 847
'. 1015 46 12.2 9.0 6.9 92. 12.2 9.6 7.3 93. 12.5 9.6 7.3 811 1016 12 12.9 8.6 7.1 93. 12.8 9.8 7.1 91. 12.3 9.8 7.1 793 1017 16 11.9 7.3 6.9 90. 11.3 9.2 7.2 89. 11.1 9.3 7.1 883 1018 24 10.9 9.8 7.0 88. 10.6 10.6 7.2 88. 10.3 10.7 7.3 910 1019 29 10.0 8.5 7.2 90. 9.3 10.1 7 2 88. 9.3 10.2 7.1 910 1020 34 9.7 9.5 7.0 90. 9.2 10.6 7.1 89. 9.1 10.8 7.3 901 1021 41 10.0 8.4 7.0 92. 9.9 10.6 7 1 88. 9.7 10.6 7.1 910 1022 46 10.5 9.4 7.1 81. 10.0 10.6 7.1 77. 9.9 10.6 7.1 3270 1023 1 10.7 8.5 6.7 70. 10.3 10.4 7.1 69. 10.2 10.4 7.0 3920 1024 12 11.3 8.8 7.0 84. 11.1 9.9 7.2 82. 11.0 10.0 7.3 3020 1025 16 11.0 7.8 6.9 85. 10.6 10.1 7.0 82. 10.4 10.2 7.3 2420 1026 24 11.0 9.2 7.0 78. 11.0 9.2 7.6 77. 10.5 10.3 7.4 2410 1027 29 10.1 9.2 6.9 71. 9.9 10.8 6.9 70. 9.8 10.8 7.0 3330 1028 34 10.5 9.0 6.9 79. 10.1 10.1 7.1 78. 10.0 10.1 7.1 3650 1029 41 9.8 8.4 6.7 73. 9.2 10.2 4.8 70. 9.1 10.3 6.9 9050 1030 46 9.8 9.4 6.8 77. 9.3 10.3 6.9 76. 9.2 10.4 6.9 10860 1031 1 10.1 8.2 6.7 84. 9.9 10.2 7.0 81. 9.8 10.3 7.0 7260 11 1 12 10.0 9.3 6.8 84. 9.6 10.5 7.0 81. 9.4 10.6 7.1 5260 11 2 16 9.1 8.2 6.5 86. 8.8 10.6 6 6 82. 8.5 10.6 6.6 4210 11 3 24 9.3 9.4 6.7 87. 9.0 10.7 6.9 82. 8.9 10.7 6.7 3820 11 4 29 9.8 7.5 6.7 83. 9.1 10.0 6.8 81. 9.3 10.1 4.9 3780 11 5 34 9.5 9.3 6.7 78. 9.0 10.2 6.5 8c. 9.0 10.2 6.9 5310 11 6 41 8.8 8.9 6.7 69. 8 5 10.4 6.7 69. 8.2 10.5 6.6 7460 11 7 46 8.7 9.8 6.9 78. 8.4 10.6 6.9 78. 8.2 10.8 6.8 7620 11 8 1 7.8 9.0 6.6 78. 7 2 11.2 6.8 76. 7.1 11.2 6.8 7040 11 9 12 7.0 10.2 6.6 78. 6.5 11.0 6.8 76. 6.3 11.1 6.6 5680 1110 16 6.3 9.3 6.8 81. 6.1 11.6 6.8 77. 5.9 11 6 6.9 4640 1111 24 6.0 11.1 6.9 79. 5.6 11.8 7.0 76. 5.3 11.8 6.7 3870 1112 29 6.1 9.8 7.0 82. 5.7 11.6 7.3 79. 5.4 11.6 7.3 3460 4 1113 34 6.0 10.8 6.8 80. 5.8 11.5 6.8 83. 5.6 11.6 6.8 3100 1114 41 5.3 10.2 6.9 80. 5 1 11.8 7.0 81. 5.0 11.7 7.0 2820 1115 46, 5.7 11.0 6.7 83. 5.1 12.0 6.8 80. 5.0 12.0 6.9 2620 1116 1 6.2 9.3 6.6 82. 6.0 11.2 6.6 77. 5.9 11.2 6.8 76. 3720 1117 12 7.5 10.1 6.7 69. 7.1 11.0 6.7 68. 7.0 11.0 6.7 68. 8220
- 1118 16 7.7 9.6 6.8 78. 7.1 11.6 7.0 79. 7.0 11.4 7.0 11400 1119 24 8.2 9.8 6.9 72. 8.1 10.7 7.0 72. 7.8 10.7 7.1 11400 1120 29 7.8 8.6 6.8 71. 7.5 10.2 6.9 73. 7.2 10.4 7.0 10800 1120 34 7.1 10.4 6.7 73. 6.9 11.1 6.7 71. 6.5 11.1'6.7 9900 1122 41 6.3 9.3 6.8 75. 6.0 10.8 6.9 73. 5.7 10.6 6.9 8300 1123 46 5.5 11.6 6 7 73. 4.9 12.4 6.7 72. 4.8 12.4 6.8 6960 1124 1 5 0 10.4 6.7 75. 4.5 12.0 6.9 73. 4.5 12.1 6.9 5840 l 1125 12 4.7 11.2 6.7 74. 4.2 11.8 6.9 74. 3.9 11.8 6.9 5020 1126 16 5.0 10.0 6.8 77. 4.8 11.3 6.9 77. 4.5 11.3 6.9 4480 1127 24 5.8 11.2 7.0 76. 5.3 11 8 7.1 75. 5.2 11.8 7.1 4880 1128 29 7.0 8.9 6.7 69. 6.8 10.9 6.8 69. 6.6 11.0 6.9 7930 1
1129 34 7.3 10.1 6.7 72. 7.0 10.9 6.8 69. 6.8 10.9 6.8 69. 10800 1130 41 7.7 8.8 6.8 70. 7.5 11.2 7.0 70. 7.1 11.0 7.1 14200 12 1 46 7.8 10.4 6.8 69. 7.2 11.0 6.9 68. 7.1 11.1 6.9 15200 12 2 1 7.7 9.1 6.8 77. 7.1 11.4 7.0 76. 7.0 11.5 7.0 14500 12 3 12 8.5 10.0 6.8 72. 8.1 11.1 6.9 60. 8.0 11.2 6.9 26400 12 4 16 8.3 8.0 6.6 60. 8.0 10.2 6.8 60. 7.8 10.2 6.9 36700 12 5 24 8.0 9.1 6.5 61. 7.8 10.4 6.7 61. 7.5 10.5 6.7 29600 12 6 29 7.5 7.9 6.5 63. 7.1 10.4 6.7 66. 7.0 10.5 6.7 29200 12 7 29 7.0 8.5 6 4 66. 6.8 10.0 6.5 65. 6.8 10.0 66. 25400 OK, e
_ ._ _ .-_ , - - _ - _ _ _ _ _ _ _ . . _ _ - . _ _ _ ~ . . . . . _ _ _ _ ~ _ _ . _ _ _._ _.
TE:.E C-2 n a a iqi" ,
, ,1 HATER C H ISTRY R1YER B B BOX CONTROL T0X l t : t l l +--t 1890 PH TSS ALK HARD 18G C0D SO4 CL PH TSS ALK HARD C0D CL PH ALK HARD 100 S04 18G PROJECT 3 24 7.1 20.0 19.0 23.0 3.7 4.5 17.0 4.0 6.9 18.8 22.0 3.4 25 6.9 19.0 22.8 .4 .8 6.7 19.6 23.4 .042 .047 1 28 7.1 22.0 24.0 .024 7.1 21.6 24.0 6.9 21.6 24.4 29 7.1 21.6 24.2 7.3 21 8 24.2 210 7.1 22.0 24.8 7.3 24.0 24.2 31 1 7.0 22.0 24.6 6.9 22.4 23.4 212 7.1 21.8 24.2 7.2 21.6 24.6 PROKCT 4
! 6.9 28.6 13.0 16.0 4.2 7.0 21.1 13.6 17.6 6.6 222 7.0 4.2 6.9 13.4 14.8 223 6.9 14.0 17.8 6.9 14.4 18.2 6.9 14.2 17.4 224 6.9 15.0 18.0 1.7 6.9 15.4 18.2 7.0 15.6 18.2 .035 4 2 .030 224 .052 224 .042 225 7.0 16.8 20.0 6.9 14.6 20.0 7.0 16.6 19.8 226 7.0 17.0 18.0 7.0 17.4 18.6 7.1 17.6 21.4 229 7.1 16.8 19.6 7.1 17.2 20.2 7.1 17.4 20.2 32 7.0 17.4 19.8 7.0 17.6 20.0 7.0 17.6 19.6 33 7.1 16.6 18.4 7.1 16.6 18.4 7.1 16.8 18.2 34 7.0 16.0 18.0 7.1 16.0 18.0 7.1 16.6 18.2 35 7.1 15.0 18.4 7.1 16.0 18.6 7.1 15.8 17.8 38 7.0 19.4 22.0 7.0 19.4 21.4 7.0 19.4 21.6 39 7.2 19.6 21.4 7.2 19.8 21.4 7.1 19.8 21.6 310 7.1 20.0 23.0 7.1 19.4 21.4 7.1 19.6 21.2 PROJECT 5 318 7.0 19.2 21.2 1.2 4.9 7.0 5.0 19.8 21.6 2.8 5.9 7.0 14.8 22.0 .064 .030 ,
318 446 '
318 .074 318 .008 319 7.1 20.4 22.0 7.1 20.6 23.0 7.1 20.6 23.2 323 .027 323 .032 323 .050 323 .092 .182 324 7.2 5.0 23.6 27 4 1.4 3.2 5.5 7.2 6.0 23.4 28.6 1.6 5.5 7.1 24.0 29.2 3.8 330 .012 330 .025 .054 l
330 .035 330 .017 331 7.2 4.3 22.4 26.4 4.8 7.0 7.1 3.0 22.5 26.2 6.5 5.0 7.1 22.6 25.4 46 .024 .010
. 46 .012
! 46 .010 l
46 .013 1 47 7.1 5.3 18.2 22.0 6.0 4.5 7.1 3.6 18.4 22.4 5.2 5.7 7.1 18.4 22.0 413 .047 .070 '
413 .040 .036 l 414 7.2 37.7 16.0 17.8 5.6 4.2 7.0 29.3 15.2 18.4 8.6 4.5 6.9 15.8 18.0 l 420 .041 .042 1 420 .033 l 420 .040 )
421 7.3 10.3 19.8 22.4 5.2 4.2 2.1 7.1 5.8 20.2 22.6 6.8 2.5 7.0 20.2 22.4 4.1 ;
427 .045 .054 l 427 .047 .057 428 7.2 4.0 23.4 25.0 5.8 3.8 7.1 2.6 23.6 26.0 5.6 3.5 7.0 24.0 25.6 54 .099 .064 54 .127 .134 55 7.2 9.3 25.0 27.6 4.8 4.7 7.2 8.0 25.2 26.8 5.8 4.6 7.1 26.0 27.2 511 .083 .052 511 .094 .048 512 7.2 1.8 26.4 29.0 2.6 4.6 7.2 0.8 26.4 28.0 2.8 4.4 7.0 26.2 29.0
N S, .t.1 f*
.m TABLE C-2 (CONTINUED)
), j g WATER CIEllISTRY (CONTIIREB)
RIYER LEAD BOX CONTROL T0X l l l l l t t--+
1888 PM TSS ALK HARB 1983 COB SO4 CL PH TSS ALK liARD COB CL PH ALK HARB 1913 SO4 1913 PROKCT 6
. 528 7.4 5.5 29.6 30.0 5.6 7.4 5.3 29.2 30.4 4.0 7.4 29.2 30.8 61 7.5 5.8 31.0 31.0 3.9 4.2 4.6 7.4 5.0 30.2 30.8 3.3 4.5 7.4 30 4 31.0 .015 4.0 .023 61 .010 61 .030 68 2.8 31.4 32.0 1.0 4.6 3.5 30.4 31.2 1.0 4.4 29 2 31.6 .031 .018 614 7.0 4.3 30.6 31.2 8.0 4.4 7.6 4.8 30.2 31.2 6.7 3.9 7.5 30 4 31.6 .013 .025 614 .059 622 7.7 6.0 31.0 31.0 13.6 4.0 7.7 6.0 31.6 31.4 11.6 4.3 7.6 31 4 31.8 .028 .018 630 7.6 6.8 31.4 31.0 10.6 2.8 4.7 7.6 5.5 30.6 31.0 14.6 4.6 7.6 30.8 31.6 .060 3.0 .012 77 7.4 8.3 30.4 32.0 8.2 4.6 7.4 7.8 30.6 31.0 10.1 4.6 7.4 30.8 31.6 .032 .036 713 7.4 3.8 30.8 31.2 4.8 5.0 7.5 2.0 31.6 31.2 5.0 5.0 7.3 31.0 32.0 .034 .030 721 7.4 2.3 31.4 31.4 4.4 4.3 7.5 2.3 32.2 32.0 6.0 3.9 7.4 32 2 33.0 .052 .040 727 7.6 2 3 31.6 32.2 6.0 4.7 7.6 1.5 32.4 32.0 6.3 4.3 7.6 324 32.8 .026 .028 PRO KCT 7 917 7.2 4.5 32.2 34.0 020 . 6.1 2.4 4.3 4.0 32.8 33.8 6.3 3.7 7.3 32.6 34.2 .020 2.3 920 7.2 26.6 31.0 7.3 23.2 29.0 7.1 23.0 23.0 921 7.2 29.4 34.2 7.3 31.4 30.8 7.3 31.0 32.0 PRO KCT 8 2023 7.2 30.8 33.2 7.3 31.0 32.4 7.2 31.2 33.4 923 7.2 29.6 34.0 7.2 31.0 33.6 7.2 30.8 32.4 10 7 7.3 '2.5 31.0 32.4 7.7 4.3 7.3 2.5 31.4 32.6 6.3 4.0 7.2 32.2 33.6 .090 .000 1013 7.5 1.8 31.0 33.4 5.7 2.5 3.8 7.3 2.3 32.0 34.0 5.1 3.6 7.2 32.4 35.6 .110 2.8 .090 1020 7.5 1.0 32.0 34.0 6.3 5.7 7.4 6.0 31.6 33.6 5.9 6.7 7.2 31.6 33.4 .070 .100 1020 .070 1027 7.2 27.8 23.0 35.8 15.6 3.8 7.1 25.8 20.0 35.4 10.7 7.0 7.0 20.8 22.6 .120 .090 11 3 .150 11 3 7.0 17.0 21.0 29.0 10.2 5.3 7.0 15.0 21.0 29.0 11.6 5.0 6.9 21.0 29.2 .000 .160 1110 7.2 1.0 20.0 26.8 10.4 4.6 4.8 7.1 1.3 20.0 26.6 10.0 3.9 7.0 20.4 27.6 .070 3.3 .000 1115 7.1 1 8 23.6 30.0 8.0 4.0 7.0 1.5 23.6 29.2 6.4 5.0 7.0 24.2 30.4 .040 .040 1115 .000 1123 7.0 4.5 18.2 25.0
- 8.8 3.2 7.0 7.5 19.2 24.0 9.9 2.9 6.9 19.4 24.0 .040 .040 1123 .060 12 1 6.9 24.7 16.0 22.8 9.8 4.4 6.9 23.7 15.6 22.4 12.0 5.0 6.8 15.6 21.8 .090 .040 12 1 .080 i
l l
1 y._ , , , _ .-. - . . - , . , _ _ ,.-,,,.y..m,_._,-,_. _
TABLE C-3 CORRELATION MATRIX OF AYERAGE HATER OUALITY PARAMETERS FOR 9-DAY LC50 TESTS ALK TSS AMMONIA C0D SO4 CL PD CA M6 K NA D.D. TEMP HARDIESS PH COND TEMP. -0.816 HARDNESS l-0.909 0.960 PH -0.849 l 0.934 0.986 4 COND l-0.960 0.931 0.984 0.949 j ALKALIN -0.839 l 0.997 0.979 0.959 0.950 ISS 0.752 -0.734 -0.872 l -0.927l -0.832 -0.785
'J,.
AMMONIA 0.545 -0.714 -0.542 -0.457 -0.566 -0.668 0.102 CDD SO4
-0.125 0.140 0.022 -0.089 0.136 0.093 0.277 -0.297 0.877 -0.516 -0.660 -0.579 -0.782 -0.547 0.565 0.233 -0.343 "K.f. .
CL 0.625 -0.477 -0.410 -0.255 -0.520 -0.443 -0.027 0.800 -0.602 0.606 PB 0.703 -0.452 -0.457 -0.323 -0.544 -0.443 0.104 0.758 -0.211 0.685 0.903 0.764 l0.906_ 0.886 0.933 0.805 -0.874 -0.363 -0.095 -0.834 CA -0.964 . -0.555 MG -0.995 0.765 '0.863 0.792l0.927 0.708 -0.691 -0.553 0.141 l-0.89? l -0.675 -0.761 l0.947l K -0.415 0.036 0.060 -0.004 0.183 0.022 0.235 -0.501 0.251 -0.641 -0.816l-0.904l 0.262 0.!04 NA l-0.956l 0.671 0.758 0.660 0.847 0.686 -0.537 -0.535 0.235 l-0.9171 -0.7si3 -0.853 0.872 l0.980l 0.657 FE 0.544 -0.807 -0.830 l-0.904 l -0.732 -0.832 l0.888l 0.235 0.248 0.203 -0.128 -0.080 -0.641 -0.456 0.487 -0.276 SIGNIFICANT YALUES EXCEED t 0.878 (0.05 LEYEL). SIGNIFICANT VALUES OUTLINED 9
I e
20.0t i= . DRAFT t
$24 ** 2 2
18.2+ 2 23 t t n t 2 82 t 2
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16.41 1 $8 5
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14.6+
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FIGiET C-2 TAliK TEMERATIEE TEMP 20.0t
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TIGURE C-4 ,
TANK DISSOLVED OXYGEN D0 13.001 t
tt 12.30t *
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- 12.0 : DECEMBER 31)
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HEAD BOX CONDUCTIVITY (AT 25 DEGREES C)
HD COND 100.H
- 95.H *
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hs J
. own FIGURE C-B TANK CONDUCTIVITY (AT 25 DEGREES C)
?)4W]s 3
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d COND 100.0+ l
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+
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APPENDIX D fl
SUMMARY
OF BIOASSAYS BY PROJECT The following tables summarize the water quality characteristics and the biological results for each project.
2 i
e 1
D-1
TABLE D-1 BI0 ASSAY AND ASSOCIATED WATER QUALITY CHARACTERISTICS ,?
-u - as aJ y Pr0 ject 3 DATE5 Febru ary ,4-8 Number Testec/ Aquaria 20 20 Metal (s) C Z Species / Life Stage CP SP CP SP LC 50 TOTAL YT 77 7U22 liBB Dissolved Labile Binding Capacity Incipient Lethal Level (number of days) 26(10) 51(10) 754(10) 213(10) tb Effect Level: M/G TEMPERATURE (C) Number (_n) 11 Mean (x) 5.5 Standard Deviation (S.D. ) 1.1 Range (R) 4.0 - 7.2 DISSOLVED OXYGEN n 11 7 12.0 S.D. 0.6 R 10.6 - 12.7 pH n 2 Median 7.0 S.D. 0.2 R 6.8 - 7.1 CONDUCTIVITY n 11 7 65-S.D. 8 R 49 - 74 ALKALINITYx n 7 I 21.4 S.D. 1.7 R 18.8 - 24.0 HARDNESSx n 7 7 23.7 S.D. 0.9 R 22.0 - 24.6 TOTAL SUSPENDED SOLIDS on 2 7 18.5 S.D. 2.1 R 17.0 - 20.0 AMMONI A-NI1ROGENX n 2 I .045 S.D. .004 R .042 .047 CHEMICAL OXYGEN DEMAND on 2 I 0.6 S.D. 0.3 SULFATEx n 1 x 3.4 S.D.
CHLORIDE n 2 7 4.3 S.D. 0.4 R 4.0 - 4.5 C - Copper Coho Parr - CP Steelhead Parr - SP Z - Zinc Coho Smoit - CS Steelhead Smoit - SS C/Z - Copper /Zine Coho Fry - CF Steelhead Fry - SF M - Mortality G - Growth x - Used Control and Toxicant Aquaria Data o - Used River and Heatox Data
_ - No Useable Information
TABLE D-2
[
810 ASSAY AND ASSOCIATED WATER QUALITY CHARACTERISTICS ,,
Project 4
" Jrey UAID Pebruary 21-25 Number Tested / Aquaria 20 40 Metal (s) C C/Z Species / Life Stage CP SP CP SP LC 50 TOTAL IIT W 'K/754 Tli/389 Dissolved l Labile Binding Capacity 6 Incipient Lethal Level (number of days) 72(9) 87(9) 31/637(9)
No Effect Level: M/G TEMPERATURE (C) Number 5 Mean 6.4
. Standard viation (S.D.) 0.5 Range (R) 6.0 - 7.3 DISSOLVED OXYGEN n 5 7 10.9 S.D. 0.6 R 9.9 - 11.4 pH n 5 Median 6.7 S.D. 0.1 R 6. 7 - 7.0 CONDUCTIVITY n 5 7 60 S.D. 4 R 53 - 63 ALKALINITYK n 5 7 15.5 S.D. 1.7 R 13.4 - 17.6 HARDNESSx n 5 7 18.3 S.D. 2.5 R 14.8 - 21.4 TOTAL SUSPENDED SOLIDS % 2 7 24.9 S.D. 5.3 R 21.1 - 28.6 AMMON! A-NITROGENE n 4 x .040 S.D. .010 4
R .030 .052 CHEMICAL OXYGEN DEMAND on 2 7 6.8 S.D. 0.3 SULFATEX n 1 ,
I 4.2 l
. S.D.
]
CHLORIDE n 2 l T 4.2 l S.D. 0.0 R 4.2 C - Copper Coho Parr - CP Steelhead Parr - SP Z - Zinc Coho Smoit - CS Steelhead Smoit - SS Coho Fry - CF Steelhead Fry - SF g/Z - Cgo g nc G - Growth x - Used Control and Toxicant Aquaria Data 0 - Used River and Headbox Data
_ - No Useable Information
TABLE D-3 B10 ASSAY AND ASSOCIATED WATER QUAL 2TY CHARACTERISTICS :, 1 4 ' 'r s,
.f *
,m t a
PROJECT 4*
DATE5 February 27-Marcn 3 Number Tested / Aquaria 20 20 Metal (s) Z Species / Life Stage CP SP LC 50 TOTAL TJ00 TU6 Dissolved Labile Binding Capacity Incipient Lethal Level (nunber of days) 849(9) 89(11)
No Effect Level: M/G TEMPERATURE (C) Number 6 ftan 7. 2 Standard Deviation (S.D. ) 0.3 Range (R) 6.8 - 7.6 DISSOLVED OXYGEN n 6 x 11.0 S.D. 0.3 R
10.5 - 11.4 pH n 6 Median 6.7 S.D. D.1 R 6.5 - 6.7 CONDUCTIVITY n 6 7 61 S.D. 4 R 53 - 64 ALKALINITYK n 8 x 17.9 S.D. 1.5 R 15.8 - 19.8 HARDNESSx _n 8 x 19.8 S.D. 1.6 R 17.8 - 21 6 TDTAL SUSPENDED SOLIDS On _
x 5.0.
R AMMONI A-NITROGENX _n x
S.D.
R CHEMICAL OXYGEN DEMAND On _
x S.D.
SULFATEx _n x
S.D.
CHLORIDE _n x
S.D.
R C - Copper Coho Parr - CP Steelhead Parr - SP Z - Zinc Coho Smoit - CS Steelhead Smolt - SS C/Z - Copper / Zinc Coho Fry - CF Steelhead Fry - SF M - Mortality G - Growth x
- Used Control and Toxicant Aquaria Data o - Used River and Headbox Data *0ne diluter started a week
_ - No useable information later than Others
TABLE 0-4 810 ASSAY AND ASSOCIATED WATER QUALITY CHARACTERISTICS ,
- , ' f1 ,;( .
1' ,
Project 5 l DATE5 Marcn 17-May 17 Number Tested / Aquaria 30 15 30 15 )
Metal (s) C Z '
Species / Life Stage CP CS SP SS CP CS SP SS LC 50 TOTAL T 89 0:4 eiz Dissolved Labile Binding Capacity 12 Incipient Lethal Level (number of days) 74(9) 87(9) 465(29) 120(29) l No Effect Level: M/G 24/22 24/45 220/326 59/179 TEMPERATURE (C) Number (jn 62 Mean (x) 9.8
- Standard Deviation (S.D.) 1.9 Range (R) 7.1 - 13.7 DISSOLVED OXYGEN n 6.2 7 9.5 S.D. 1.1 R 6.9 - 11.8 pH n 60 Meoian 7.0 R' 6.8' 7.2 CONDUCTIVITY n 61 7 73 S.Q. 5 R 61 - 82 ALKALINITYX n 10 7 21.3 S.D. 4.0 R 14.8 - 26.2 HARDNESSx n 10 7 24.4 S.D. 3.5 R 18.0 - 29.2 TOTAL SUSPENDED SOLIDS On 17 7 8.3 S.D. 9.9 t R 0.8 - 37.7 l
l AMMONI A-NI TROGENX n 23 x .055
! S.D. .037 R .010 .134 l CHEMICAL OXYGEN DEMAND on 18 l x 4.7 '
l S.D. 2.1 t
l SULFATEX n 2 7 4.0 i S.D. 0.2 1
CHLORIDE n 18 )
I 4.4 S.D. 1.2 l R 2.1 - 7.0 C - Copper Coho Parr - CP Steelhead Parr - SP Z - Zinc Coho Smoit - CS Steelhead Smoit - SS
- C/Z - Copper / Zinc Coho Frj - CF Steelhead Fry - SF M - Mortality G - Growth x - Used Control and Toxicant Aquaria Data o - Used River and Headbox Data
_ - No Useable Information
TABLE D-5 810 ASSAY AND ASSOCIATED WATER QUALITY CHARACTERISTICS e.a a d is aj Project 6S UMD May 24-July 28 Number Tested / Aquaria 30 C
Metal Species(s)
/L ife Stage CF LC 50 TOTAL Dissolved Labile Binding Capacity 16 Incipient Lethal Level (nunter of days) 62(9) 39(60)
No Effect Level: M/G 18/21 TEMPERATURE (C) Number 65 Mean 16.9 Standard eviation (S.D.) 1.5 Range (R) 14 - 19.6 DISSOLVED OXYGEN n 65 I 9.0 S.D. 0.7 R 7.7 - 10.4 pH n 65 Median 7.2 S.D. 0.1 R 6.9 - 7.4 CONOUCTIVITY n 62 7 86 S.0. 3 R 79 - 91 ALKALINITY 8 n 10 I 30.8 S.0. 1.1 R 29.2 - 32.2 HARDNES$x n 10 7 31.8 S.D. 0.7 R 30.8 - 33.0 TOTAL SUSPENDED SOLIDS On 20 7 4.6 S.D. 1.9 R 1.5 - 8.3 AMMONI A-N!1ROGEN8 n 21 x .030 5.0. .014 R .010 .060
, CHEMICAL OXYGEN DEMAND on 20 x 6.7 S.D. 3.8 SULFATE 8 n 2 7 3.5 S.D. 0.7 CHLORIDE n 18 7 4.5 S.D. 0.3 R 3.9 - 5.0 C - Copper Coho Parr - CP Steelhead Parr - SP Z - Zinc Coho Smoit - CS Steelhead Smoit - SS C/Z - Copper /Zine Coho Fry - CF Steelhead Fry - SF M - Mortality G - Growth x - Used Control and Toxicant Aquarta Data 0 - Used River and Heaeon Data
_ - No Useable Information
TABLE D-6 j V
810 ASSAY AND ASSOCIATED WATER QUALITY CHARACTERISTICS Project 6 uniu may a-June i Number Tested / Aquaria 30 20 30 15 Metal (s) C C/Z Species / Life Stage CF CS CF SS LC 50 TOTAL . ET II 3T/160 U /128 Dissolved Lablie I Binding Capacity i Incipient Lethal Level (number of days) 61(9) 61(9)47/137(11) '
No Effect Level: M/G TEMPERATURE (C) Numoer 5 Mean 15.5 Standard viation (S.D.) 0.9
. Range (R) 14.0 - 16.2 DISSOLVED OXYGEN n 5 i 7 9.6 S.D. 0.3 R 9.3 - 9.8 pH n 5 Median 7.2 S.D. 0.2 R 6.9 - 7.4 CONDUCTIVITY n 5 7 82 S.D. 1 R 81 - 84 ALKALINITY 8 n 3 7 29.6 S.D. 0.7 R 29.2 - 30.4 HARDNESSx n 3 7 31.1 S.D. 0.4 R 30.8 - 31.6 TOTAL SUSPENDED SOLIDS On 6 7 4.7 S.D. 1.2 R 2.8 - 5.8
! AMMONI A-N! TROGEN8 n 6 l 7 .021 S.0. .008 R .010 .031 CHEMICAL OXYGEN DEMAND On 6 l T 3.1 (
S.D. 1.8 !
l SULFATEx n 1 7 4.0 S.D. I CHLORIDE n 4 l 7 4.5 l S.D. 0.1 R 4.4 - 4.6 C - Copper Coho Parr - CP Steelhead Parr - SP Z - Zinc Coho Smoit - CS Steelhead Smoit - SS C/Z - Copper / Zinc Coho Fry - CF Steelhead Fry - SF M - Mortality G - Growth x - Used Control and Toxicant Aquaria Data o - Used River and Heaeox Data
_ - No Useable information
TABLE D-7 8!0 ASSAY AND ASSOCIATED WATER QUALITY CHARACTERISTICS j' ,a Ilah ] 's j $4 d Project 6A UAID June lO-June 14 Number Tested / Aquaria 30 10 30 10 Metal (s) C C/Z Species / Life Stage CF CS CF CS LC 50 TOTAL E W 77/166 77/171 Dissolved Labile Bindin9 Capacity Incipient Lethal Level (number of days) 82(9) 84(9) 72/159(11)76/166(11)
No Effect Level: M/G TEMPERATURE (C) Number (n) 4 Mean (x) 16.2 Standard Deviation (5.0.) 1.0 Range (R) 14.8 - 17.0 -
DISSOLVED OXYGEN n 4 7 9.5 S.D. 0.6 R 8.7 - 10.0 pH n 4 Median 7.2 S.D. 0.1 R 7.1 - 7.2 CONDUCTIVITY n 4 7 86 S.D. 1 R 84 - 87 ALKALINITY
- n 1 7 30.4 S.D.
R HAR DNES58 n 1 I 31.6 S.D.
R TOTAL SUSPENDED SOLIDS on 2 7 4.6 S.D. 0.4 R 4.3 - 4.8 AMMON! A-N! TROGENX n 3 Y .032 5.D. .024 R .013 .059
) CHEMICAL OXYGEN DEMAND on 2 7 7.4 S.D. 0.9 SULFATE
- _n X
S.D.
CHLORIDE n 2 7 4.2 S.D. 0.4 R 3.9 - 4.4 C - Copper Coho Parr - CP Steelhead Parr - SP Z - Zine Coho Smolt - CS Steelhead Smoit - SS C/Z - Copper / Zinc Coho Fry - CF Steelhead Fry - SF M - Mortality G - Growth x - Used Control and Toxicant Aquaria Data o - Used River and Hea ton Data
_ - No Useable Information
TABLE D-8 BI0 ASSAY AND ASSOCIATED WATER QUALITY CHARACTERISTICS ,
uu .:n u
Project 7 ~
uAits september ad-zu Numoer Tested / Aquaria 30 30 30 30 Metal (s) C C/Z Species / Life Stage CP SP CP SP LC 50 TOTAL TU3 W N/267 W/214 Dissolved Labile Binding Capacity 18 Incipient Lethal Level (number of days) 69(9) 55(9) 72/224(9) 67/206(9)
No Effect Level: M/G TEMPERATURE (C) Number n 7 lean x 15.4
- Standard viation (S.D. ) 0.3 Range (R) 15.2 - 16.0 DISSOLVED OXYGEN n 7 7 8.4 S.D. 0.6 R 7.3 - 9.2 pH n 7 Median 7.2 S.D. 0.1 R 7.0 - 7.2 CONDUCTIVITY n 7 7 92 S.D. 3 R 89 - 97 ALKALINITYX n 5 x 29.7 S.D. 3.8 R 23.0 - 32.G l HARDNESSx n 5 x 31.0 S.D. 4.6 R 23.0 - 34.2 TOTAL SUSPENDED SOLIDS On 2 7 4.3 S.0. 0.4 R 4.0 - 4.5 AMMONI A-NITROGENX n 1 x .020 5.0.
R CHEMICAL OXYGEN DEMAND on 2 7 6.2 S.0. 0.1 SULFATEX n 1 7 2.3 ;
S.D. 1 CHLORIDE n 2 ,
7 3.1 S.D. 0.9 R 2.4 - 3.7 C - Copper Coho Parr - CP Steelhead Parr - SP Z - Zinc Coho Smoit - CS Steelhead Smolt - SS C/Z - Copper /Zine Coho Fry - CF Steelhead Fry - SF M - Mortality G - Growth x - Used Control and Toxicant Aquaria Data o - Used River and Heatox Data
_ - No Useable Information
TABLE D-9 BI0 ASSAY AND ASSOCIATED WATER QUALITY CHARACTERISTICS OilDS f'- ; V.= W I
LO $ 1J b b Project 8 UAID Uctober 6-Decee er 6 Number Tested / Aquaria 30 20 30 20 Metal (s) C C/Z Species / Life Stage CP SP CP SP LC 50 TOTAL Dissolved Labile Binding Capacity 16 incipient Lethal Level (number of days)
No Effect Level: M/G 56 28 32/80 z8/70 7I TE 7577U Tb7TTO TEMPERATURE (C) Number 67 Mean 9.2 Standard Deviation (S.D.) 2.6 Range (R) 13.8 - 4.7 DISSOLVED OXYGEN n 6.7 7 9.1 S.D. 1.0 R ' 7.3 - 11.6 pH n 6.7 Median 6.8 S.D. 0.2 R 6.4 - 7.2 CONDUCTIVITY n 66 7 80 5.0. 9 R M - 95 ALKALINITYx n 9 x 24.2 S.D. 6.3 R 15.6 - 32.4 HARDNESSX n 9 7 28.7 S.D. 5.1 R 21.8 - 35.6 TOTAL SUSPENDED SOLIDS On 18 I 9.3 S.D. 10.0 R 1.0 - 27.8 AMMONI A-NITROGENX n 23 7 .081 S.D. .033 R .040 . 160 I CHEMICAL OXYGEN DEMAND On 18 7 8.9 S.D. 2.7 SULFATEx n 2 I 3.1 S.D. 0.4 CHLORIDE n 18 7 4.6 S.D. 1.1 R 2.9-7.0 C - Copper Coho Parr - CP Steelhead Parr - SP Z - Zinc Coho Smoit - CS Steelhead Smolt - SS C/Z - Copper / Zinc Coho Fry - CF Steelhead Fry - SF M - Mortality G - Growth x - Used Control and Toxicant Aquaria Data o - Used River and Hea sox Data
_ - No Useable Information
APPENDIX E SPEARMAN-KARBER CALCUIATIONS The following tables summarize the Spearman-Karber calculations performed on the mortality data.
Errata:
Each summary table has a line which reads "the proportion dead in 96 hours0.00111 days <br />0.0267 hours <br />1.587302e-4 weeks <br />3.6528e-5 months <br /> were". This line should read "the proportion dead"; the time frame is defined on the first line.
i E-1
'6 PROJECT 3 -
e
COHO SMOLT (73 HOURS)
COPPER FEBRUARY 4e 1982 PROJECT 3, DILUTER A 10% SK NOT CALCULABLE COHO SMOLT (77 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 10% SK NOT CALCULABLE COHO SHOLT (82 HOURS) -
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 103 SK NOT CALCULABLE COHO SMOLT (93 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1500 0.6500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 84 THE 95% CONFIDENCE INTERVAL IS 79 89 COHO SMOLT (96 HOURS)
- FEBRUARY 4, 1982 PROJECT 3, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.2500 0.7500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 81 THE 95% CONFIDENCE INTERVAL IS 76 86
1 l
COHO SMOLT (99 HOURS) i COPPER -
FEBRUARY 4, 1982 l PROJECT 3, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS '
THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 '110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.2500 0.8500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 79 THE 95% CONFIDENCE INTERVAL IS 75 83 l
COHO SMOLT (118 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.5000 0.9500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 71 THE 95% CONFIDENCE INTERVAL IS 57 87 COHO SMOLT (124 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS l THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 l THE PROPORTION DEAD IN 96 HOURS WERE 0 0000 0.1500 0.5500 0.9500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 70 d THE 95% CONFIDENCE INTERVAL IS 56 86 l
l COHO SMOLT (142 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.3500 0.9500 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 45 THE 95% CONFIDENCE INTERVAL IS 27 75 l
COHO SHOLT (168 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.4500 0.9500 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 36 THE 95% CONFIDENCE INTERVAL IS 21 61 COHO SMOLT (226 HOURS) -
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5000 1.0000 1.0000 1.0000 1.0000 THE SPEARMAN KARSER LC50 ESTIMATE IS 32 THE 95% CONFIDENCE INTERVAL IS 19 55 COHO SMOLT (248 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A
- 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.6000 1.0000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 26 THE 95% CONFIDENCE INTERVAL IS 16 43
COHO SHOLT (73 HOURS) !
COPPER l FEBRUARY 4, 1982 !
PROJECT 3, DILUTER A i 15% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (77 HOURS)
COPPER FEBRUARY 4, 1982 l PROJECT 3, DILUTER A l 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.3500 0.8500 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 94 THE 95% CONFIDENCE INTERVAL IS 88 100 COHO SMOLT (82 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.5500 0.8500 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 88 THE 95% CONFIDENCE INTERVAL IS . 82 95 COHO SHOLT (93 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS ,
THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1500 0.6500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 84 THE 95% CONFIDENCE INTERVAL IS 80 8 'i
i COHO SMOLT (96 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.2500 0.7500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 81 THE 95% CONFIDENCE INTERVAL IS 76 86 COHO SMOLT (99 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.2500 0.8500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 79 THE 95% CONFIDENCE INTERVAL IS 75 84 COHO SMOLT (118 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 .20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.5000 0.9500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 73 THE 95% CONFIDENCE INTERVAL IS 69 78 COHO SHOLT (124 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.5500 0.9500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 72 THE 95% CONFIDENCE INTERVAL IS 68 77
COHO SMOLT (142 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.3500 0.9500 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 49 THE 95% CONFIDENCE INTERVAL IS 26 94 COHO SMOLT (168 HOURS)
COPPER .
FEBRUARY 4, 1982 l PROJECT 3, DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.4500 0.9500 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 40 THE 95% CONFIDENCE INTERVAL IS 21 74 COHO SMOLT (226 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS TliE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5000 1.0000 1 0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 35 )
THE 95% CONFIDENCE INTERVAL IS 19 65 i
COHO SMOLT (248 HOURS)
COPPER l FEBRUARY 4, 1982 !
PROJECT 3, DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.6000 1.0000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 28 THE 95% CONFIDENCE INTERVAL IS 15 50
COHO SHOLT (73 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3 DILUTER A 30% TRIMMED SPEARMAH-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.3500 0.8500 0.8500 THE SPEARMAN KARBER LC50 ESTIMATE IS 94 THE 95% CONFIDENCE INTERVAL IS 87 102 COHO SMOLT (77 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3 DILUTER A 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER B.ILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20
.THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.3500 0.8500 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 94 THE 95% CONFIDENCE INTERVAL IS 87 102 COHO SMOLT (82 HOURS)
COPPER FEBRUARY 4, 1987 PROJECT 3, DILUTER A 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER DILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.5500 0.8500 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 88 THE 95% CONFIDENCE INTERVAL IS 81 95 t
COHO SHOLT (93 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1500 0.6500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 84 THE 95% CONFIDENCE INTERVAL IS 79 89
COHO SMOLT (96 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.2500 0.7500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 81 THE 95% CONFIDENCE INTERVAL IS 76 85 COHO SMOLT (99 HOURS) 1 COPPER i FEBRUARY 4, 1982 l PROJECT 3, DILUTER A 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 l THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.2500 0.8500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 79 THE 95% CONFIDENCE INTERVAL IS 74 86 i
COHO SMOLT (118 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121
- THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 l THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.5000 0.9500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 73 THE 95% CONFIDENCE INTERVAL IS 69 78 I i
COHO SMOLT (124 HOURS)
COPPER FEBRUARY 4, 1982 j PROJECT 3, DILUTER A 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.5500 0.9500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 72 THE 95% CONFIDENCE INTERVAL IS 68 7/
l
COHO SHOLT (142 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.3500 0.9500 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 54 ,
THE 95% CONFIDENCE INTERVAL IS 23 125 C0HO SHOLT (168 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 203 TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.4500 0.9500 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 43 THE 95% CONFIDENCE INTERVAL IS 20 95 COHO SMOLT (226 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER,0F FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5000 1.0000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 38 THE 95% CONFIDENCE INTERVAL IS 18 82 COHO SMOLT (248 HOURS)
COPPER FEBRUARY 4, 1982 PROJECT 3, DILUTER A 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.6000 1.0000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 29 THE 95% CONFIDENCE INTERVAL IS 14 60
I e
l COHO SMOLT (73 HOURS)
ZINC FEBRUARY 4, 1982 PROJECT 3 DILUTER B l l
10% TRIMMED SPEARMAN-KARBER ANALYSIS I
( THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 l l THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 ;
l THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.1000 0.9500 1 THE SPEARMAN KARBER LC50 ESTIMATE IS 2 121 THE 95% CONFIDENCE INTERVAL IS 1977 2 276 l
l COHO SMOLT (93 HOURS) l ZINC
)
i FEBRUARY 4, 1982 i PROJECT 3r DILUTER B l 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.1500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 2022 THE 95% CONFIDENCE INTERVAL IS 1704 2 400 COHO SMOLT (99 HOURS)
ZINC FEBRUARY 4, 1982 PROJECT 3 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS l THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 l IHE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0500 0.1500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 2015 THE 95% CONFIDENCE INTERVAL IS 1720 2360 l
COHO SMOLT (118 HOURS)
ZINC .
FEBRUARY 4, 1982 I PROJECT 3 DILUTER B l 10% TRIMMED SPEARMAN-KARBER ANALYSIS i i
THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 l THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 2'O THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.4500 0.3000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1 278 THE 95% CONFIDENCE INTERVAL IS 967 1688 v . ~ . . . - - _
COHO SHOLT (142 HOURS)
ZINC FEBRUARY 4, 1982 PROJECT 3, DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.4500 0.4000 1.0000 THE EPEARHAN KAREER LC50 ESTIMATE IS 1193 THE 95% CONFIDENCE INTERVAL IS 904 1574 COHO SHOLT (150 HOURS)
ZINC FEBRUARY 4, 1982 PROJECT 3, DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.4500 0.4500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1142 THE 95% CONFIDENCE INTERVAL IS 863 1509 COHO SMOLT (168 HOURS)
ZINC FEBRUARY 4, 1982 PROJECT 3, DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTIDH DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.5500 0.7000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 825 THE 95% CONFIDENCE INTERVAL IS 630 1082 COHO SHOLT (173 HOURS)
ZINC FEBRUARY 4, 1982 PROJECT 3, DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.6500 0.7000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 754 THE 95% CONFIDENCE INTERVAL IS 579 983
r l
l COHO SMOLT (73 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE' 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.1000 0.9500 THE SPEARM KARBER LC50 ESTIMATE IS 2121 THE 95% C0hrIDENCE INTERVAL IS 1977 2 276 COHO SMOLT (93 HOURS)
ZINC FEBRUARY 4e 1982 DILUTER B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.1500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 2034 THE 95% CONFIDENCE INTERVAL IS 1881 2 200 COHO SMOLT (99 30URS)
ZINC FEBRUARY 4, 1982 DILUTER B 15% TRIMMED SPEARMAN-KARBER ANALYSIS
( THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 t
THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0500 0.1500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 2034 i THE 95% CONFIDENCE INTERVAL IS 1881 2 200 COHO SMOLT (118 HOURS)
ZINC 4 FEBRUARY 4, 1982 l DILUTER B 15% TRIMMED SPEARMAN-KARBER ANALYSIS ,
THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.4500 0.3000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1320 THE 95% CONFIDENCE INTERVAL IS 970 1798 l
)
l l
COHO SMOLT (142 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.4500 0.4000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1215 THE 95% CONFIDENCE INTERVAL IS 892 1654 COHO SMOLT (150 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 15% TRIMMED SPEARMAN-KARBER ANALYSIS
'THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.4500 0.4500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1157 THE 95% CONFIDENCE INTERVAL IS 847 1578 COHO SMOLT (168 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.5500 0.7000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 812 THE 95% CONFIDENCE INTERVAL IS 604 1091 COHO SHOLT (173 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.6500 0.7000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 733 THE 95% CONFIDENCE INTERVAL IS 548 979
^^
1 l
COHO SMOLT (73 HOURS) -
ZINC ,
FEBRUARY 4, 1982 PROJECT 3, DILUTER B '
20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.1000 0.9500 THE SPEARMAN KARBER LC50 ESTIMATE IS 2121 THE 95% CONFIDENCE INTERVAL IS 1977 2 276 C0HO SHOLT (93 HOURS)
ZINC FEBRUARY 4, 1982 PROJECT 3, DILUTER 8 ;
20% TRIMMED SPEARMAN-KARBER ANALYSIS )
THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 i THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.1500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 2034 THE 95% CONFIDENCE INTERVAL IS 1881 2 200 COHO SMOLT (99 HOURS)
ZINC FEBRUARY 4, 1982 PROJECT 3, DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF TISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0500 0.1500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 2034 THE 95% CONFIDENCE INTERVAL IS 1881 2 200 COHO SMOLT (118 HOURS) 2INC i FEBRUARY 4, 1982 ,
PROJECT 3, DILUTER B !
20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.4500 0.3000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1371 THE 95% CONFIDENCE INTERVAL IS 972 1933
COHO SMOLT (142 HOURS)
ZINC FEBRUARY 4, 1982 PROJECT 3, DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.4500 0.4000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1241 THE 95% CONFIDENCE INTERVAL IS 878 1754 COHO SMOLT (150 HOURS)
ZINC FEBRUARY 4, 1982 PROJECT 3, DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.4500 0.4500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1174 THE 95% CONFIDENCE INTERVAL IS 828 1665 COHO SMOLT (168 HOURS)
ZINC .
FEBRUARY 4, 1982 PROJECT 3, DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.5500 0.7000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 796 THE 95% CONFIDENCE INTERVAL IS 575 1101 COHO SHOLT (173 HOURS)
. ZINC FEBRUARY 4, 1982 PROJECT 3, DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.6500 0.7000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 708 THE 95% CONFIDENCE INTERVAL IS 515 973
STEELHEAD SHOLT (96 HOURS)
COPPER FEBRUARY 4, 1982 DILUTER A 10% SPEARMAN-KARBER NOT CALCULABLE STEELHEAD SMOLT (118 HOURS)
COPPER FEBRUARY 4, 1982 DILUTER A 10% SPEARMAN-KARBER NOT CALCULABLE STEELHEAD SMOLT (142 HOURS) .
COPPER FEBRUARY 4, 1982 DILUTER A 10% SPEARMAN-KARBER NOT CALCULABLE STEELHEAD SMOLT (150 HOURS)
COPPER FEBRUARY 4e 1982 DILUTER A 10% SPEARMAN-KARBER NOT CALCULABLE STEELHEAD SMOLT (168 HOURS)
COPPER FEBRUARY 4e 1982 DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.7000 0.8500 0.9500 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 69 l THE 95% CONFIDENCE INTERVAL IS 55 85 I
STEELHEAD SMOLT (173 HOURS) ;
I COPPER FEBRUARY 4, 1982 DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.2000 0.7000 0.8500 0.9500 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 64 THE 95% CONFIDENCE INTERVAL IS 46 87
STEELHEAD SMOLT (190 HOURS)
COPPER FEBRUARY 4, 1982 ,
DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.2500 0.7000 0.9000 0.9500 0.9500 THE SPEARMAN KARBER LC50 ESTIMATE IS 57 THE 95% CONFIDENCE INTERVAL IS 39 83 STEELHEAD SHOLT (226 HOURS)
COPPER FEBRUARY 4, 1982 DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.2500 0.7500 0.9000 0.9500 0.9500 THE SPEARMAN KARBER LC50 ESTIMATE IS 57 THE 95% CONFIDENCE INTERVAL IS 39 82 STEELHEAD SMOLT (248 HOURS)
COPPER FEBRUARY 4, 1982 DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE ?R0 PORTION DEAD IN 96 HOURS WERE 0.0000 0.3000 0.8000 0.9000 0.9500 0.9500 THE SPEARMAN KARBER LC50 ESTIMATE IS 51 THE 95% CONFIDENCE INTERVAL IS 34 77
STEELHEAD SMOLT (96 HOURS)
COPPER FEBRUARY 4, 1982 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 l THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 l THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.4000 0.8000 0.9000 ;
THE SPEARMAN KARBER LC50 ESTIMATE IS 93 l THE 95% CONFIDENCE INTERVAL IS 86 100 STEELHEAD SMOLT (118 HOURS)
COPPER FEBRUARY 4, 1982 i DILUTER A 1 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.3500 0.5500 0.8500 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 84 THE 95% CONFIDENCE INTERVAL IS 78 92 STEELHEAD SMOLT (142 HOURS)
COPPER FEBRUARY 4, 1982 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 l THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.5000 0.8000 0.8500 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 75 i THE 95% CONFIDENCE INTERVAL IS 69 81 l
STEELHEAD SMOLT (150 HOURS)
COPPER !
FEBRUARY 4, 1982 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 l THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 I THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.6000 0.8000 0.8500 0.9000 i
THE SPEARMAN KARBER LC50 ESTIMATE IS 73 THE 95% CONFIDENCE INTERVAL IS 47 79
1 i
STEELHEAD SMOLT (168 HOURS)
COPPER FEBRUARY 4, 1982 DILUTER A 13% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.7000 0.8500 0.9500 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 70 THE 95% CONFIDENCE INTERVAL IS 66 75 STEELHEAD SHOLT (173 HOURS)
COPPER FEBRUARY 4, 1982 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.2000 0.7000 0.8500 0.9500 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 48 THE 95% CONFIDENCE INTERVAL IS 54 85 STEELHEAD SMOLT (190 HOURS)
COPPER FEBRUARY 4e 1982 DILUTER A 19% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.2500 0.7000 0.9000 0.9500 0.9500 THE SPEARMAN KARBER LC50 ESTIMATE IS 63 THE 95% CONFIDENCE INTERVAL IS 45 87 STEELHEAD SHOLT (226 HOURS)
COPPER FEBRUARY 4, 1982 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.2500 0.7500 0.9000 0.9500 0.9500 THE SPEARMAN KARBER LC50 ESTIMATE IS 62 THE 95% CONFIDENCE INTERVAL IS 44 86
l l
STEELHEAD SMOLT (248 HOURS)
COPPER FEBRUARY 4, 1982 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 62 74 88 110 121
)
THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 i THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.3000 0.8000 0.9000 0.9500 0.9500 f THE SPEARMAN KARBER LC50 ESTIMATE IS 56 THE 95% CONFIDENCE INTERVAL IS 37 04 '
I
l STEELHEAD SMOLT (25 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0 0000 0.0000 0.0000 0.2000 0.9500
~
THE SPEARMAN KARBER LC50 ESTIMATE IS 1985 THE 95% CONFIDENCE INTERVAL IS 1656 2380 STEELHEAD SHOLT (28 HOURS)
IINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2500 0.4000 0.3000 1 0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1169 THE 95% CONFIDENCE INTERVAL IS 847 1615 STEELHEAD SMOLT (43 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.0000 0.0500 0.6000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1374 THE 95% CONFIDENCE INTERVAL IS 1134 1664 STEELHEAD SMOLT (47 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.0000 0.0500 0.7500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1212 THE 95% CONFIDENCE INTERVAL IS 1031 1425
STEELHEAD SMOLT (57 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.0000 0.1000 0.9500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1046 THE 95% CONFIDENCE INTERVAL IS 975 1122 STEELHEAD SHOLT (73 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.1500 0.3500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 786 THE 95% CONFIDENCE INTERVAL IS 616 1003 STEELHEAD SMOLT (77 HOURS)
ZINC FEBRUARY 4e 1982 DILUTER B 10% TRIMMED SPEARMAH-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0 2000 0.3500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 752 THE 95% CONFIDENCE INTERVAL IS 584 969 STEELHEAD SMOLT (82 HOURS)
ZINC FEBRUARY 4, 1982 DILUTE 1 B 10% TRIMMED SPEARMAN-KARBER ANALYSIL THE DOSES (PARTS PER BILLION) WERE 11 102 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERI 0.0000 0.1000 0.2000 0.4000 1.0000 1.0000 l
THE SPEARMAN KARBER LC50 ESTIMATE IS 719 THE 95% CONFIDENCE INTERVAL IS 556 930
STEELHEAD SMOLT (96 HOURS.5 ZINC -
FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 ' 1319 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORT, ION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2500 0.4000 1.0000 1.0000 THE SPEARMed KARBER LC50 ESTIMATE IS 688 THE 95% CONFIDENCE INTERVAL IS 528 898 STEELHEAD SMOLT (99 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 340 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2500 0.4000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 654 THE 95% CONFIDENCE INTERVAL IS 465 921 STEELHEAD SMOLT (118 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.3000 0.3500 0.4500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 438 THE 95% CONFIDENCE INTERVAL IS 268 715 STEELHEAD SMOLT'(124 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES :(PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.3000 0.3500 0.4500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 438 THE 95% CONFIDENCE INTERVAL IS 268 715
l STEELHEAD SMOLT (142 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5000 0.3500 0.6000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 278 -
THE 95% CONFIDENCE INTERVAL IS 163 475 STEELHEAD SMOLT (168 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5500 0.4000 0.6000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 240 THE 95% CONFIDENCE INTERVAL IS 139 413 STEELHEAD SMOLT (173 HOURS)
. FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5500 0.4000 0.6500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 230 THE 95% CONFIDENCE INTERVAL IS 134 395 s
STEELHEAD SHOLT (173 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5500 0.4500 0.6500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 213 !
THE 95% CONFIDENCE INTERVAL IS 124 367
ae STEELHEAD SMOLT (25 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3008 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.2000 0.9500 THE SPEARMAN KARBER LC50 ESTIMATE IS 2017 THE 95% CONFIDENCE INTERVAL IS 1811 2 248 STEELHEAD SMOLT (28 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLIGN) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2500 0.4000 0.3000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1342 THE 95% CONFIDENCE INTERVAL IS 905 1990 STEELHEAD SHOLT (43 HOURS)
ZINC FEBRUARY 4, 1982
- DILUTER B 20% TRIMMED SPEARMAN-KARBER AN'ALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.0000 0.0500 0.6000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1359 THE 95% CONFIDENCE INTERVAL IS 1072 1722 l
STEELHEAD SM0LT (47 HOURS)
( ZINC FEBRUARY 4, 1982 j DILUTER B l
20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.0000 0.0500 0.7500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1186 THE 95% CONFIDENCE INTERVAL IS 1006 1397
l l
l l STEELHEAD SMOLT (57 HOURS)
ZINC FEBRUARY 4, 1982 r DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.0000 0.1000 0.9500 1.0000 THE CPEARMAN KARBER LC50 ESTIMATE IS 1046 THE 95% CONFIDENCE INTERVAL IS 975 1122 STEELHEAD SMOLT (73 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.1500 0.3500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIllATE IS 842 THE 95% CONFIDENCE INTERVAL IS 650 1090 STEELHEAD SMOLT (77 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAH-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2000 0.3500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 823 THE 95% CONFIDENCE INTERVAL IS 629 1075 STEELHEAD SMOLT (82 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2000 0.4000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 777 THE 95% CONFIDENCE INTERVAL IS 592 1019
STEELHEAD SMOLT (96 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH JN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2500 0.4000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 746 THE 95% CONFIDENCE INTERVAL IS 549 1015 STEELHEAD SMOLT (99 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPEDTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2500 0.4000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 743 THE 95% CONFIDENCE INTERVAL IS 539 1024 STEELHEAD SMOLT (118 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.3000 0.3500 0.4500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 538 THE 95% CONFIDENCE INTERVAL IS 319 906 I
)
STEELHEAD SMOLT (124 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.3000 0.3500 0.4500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 538 THE 95% CONFIDENCE INTERVAL IS 319 906
STEELHEAD SMOLT (142 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5000 0.3500 0.6000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 318 THE 95% CONFIDENCE INTERVAL IS 169 599 STEELHEAD SHOLT (168 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAH-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5500 0.4000 0.6000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 265 THE 95% CONFIDENCE INTERVAL IS 138 512 STEELHEAD SMOLT (173 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE HUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5500 0.4000 0.6500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 252 THE 95% CONFIDENCE INTERVAL IS 131 484 STEELHEAD SMOLT (173 HOURS)
ZINC FEBRUARY 4, 1982 DILUTER B 20% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 11 182 360 751 1519 3088 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5500 0.4500 0.6500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 230 THE 95% CONFIDENCE INTERVAL IS 119 445
y --
l
) PROJECT 4 1
i i
i
COHO SMOLT (64 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A 10% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (72 HOURS) .
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.0500 0.9500 THE SPEARMAN KARBER LC50 ESTIMATE IS 92 THE 95% CONFIDENCE INTERVAL IS 90 94 COHO SMOLT (89 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.1500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 90 THE 95% CONFIDENCE INTERVAL IS 85 95 COHO SMOLT (92 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.3000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 86 THE 95% CONFIDENCE INTERVAL IS 81 92 f
e
COHO SHOLT (96 HOUSS)
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.3500 1.0000 THE SPEARMAN KARBER LC50 ESTIHATE IS 85 THE 95% CONFIDENCE INTERVAL IS 80 91 COHO SMOLT (112 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.5000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 81 THE 95% CONFIDENCE INTERVAL IS 76 87 COHO SMOLT (117 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAH-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0455 0.5500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 80 THE 95% CONFIDENCE INTERVAL IS 75 35 COHO SMOLT (136 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAH-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0909 0.5500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 79 THE 95% CONFIDENCE INTERVAL IS 74 85
^
l l
COHO SMOLT (164 HOURS)
COPPER -
FEBRUARY 21, 1982 PROJECT 4, DILUTER A )
10% TRIMMED SPEARMAN-KARBER ANALYSIS i 4THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1818 0.6000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 77 THE 95% CONFIDENCE INTERVAL IS 71 83 COHO SMOLT (169 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1818 0.6500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 76 THE 95% CONFIDENCE INTERVAL IS 70 81 COHO SHOLT (186 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1818 0.7000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 74 THE 95% CONFIDENCE INTERVAL IS 69 80 COHO SMOLT (209 HOURS)
, COPPER FEPRUfRY 21, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.2273 0.7500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 73 THE 95% CONFIDENCE INTERVAL IS 68 78
COHO SMOLT (213 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.2727 0.7500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 72 THE 95% CONFIDENCE INTERVAL IS 67 77 COHO SMOLT (239 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4, DILUTER A -
10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.2727 0.8500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 70 THE 95% CONFIDENCE INTERVAL IS 66 74 i
e
~
COHO SMOLT (64 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.0500 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 93 THE 95% CONFIDENCE INTERVAL IS 90 95 COHO SMOLT (72 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.0500 0.9500 THE SPEARMAN KARBER LC50 ESTIMATE IS 92 THE 95% CONFIDENCE INTERVAL IS 90 94 COHO SMOLT (89 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.1500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 90 THE 95% CONFIDENCE INTERVAL IS 88 92 COHO SMOLT (92 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROP 0RTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.3000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 87 THE 95% CONFIDENCE INTERVAL IS 80 94
1 COHO SMOLT (96 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A i 15% TRIMMED SPEARMAN-KARBER ANALYSIS l THE DOSES (PARTS PER BILLION) WERE 03 43 54 63. 82 103 I THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.3500 1.0000 l
THE SPEARMAN KARBER LC50 ESTIMATE IS 85 l THE 95Z CONFIDENCE INTERVAL IS 79 93 COHO SHOLT (112 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.5000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 81 THE 95% CONFIDENCE INTERVAL IS 75 88 i
COHO SMOLT (117 HOURS)
( FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0455 0.5500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 80 THE 95% CONFIDENCE INTERVAL IS 74 86 l
l COHO SMOLT (136 HOURS) l COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0909 0.5500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 79 i THE 95% CONFIDENCE INTERVAL IS ,73 86 l _ .__ _
COHO SMOLT (164 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1818 0.6000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 77 THE 95% CONFIDENCE INTERVAL IS 71 83 COHO SMOLT (169 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1818 0.6500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 76 THE 95% CONFIDENCE INTERVAL IS 70 82 COHO SMOLT (184 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1818 0.7000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 74 THE 95% CONFIDENCE INTERVAL IS 69 80 COHO SMOLT (209 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.2273 0.7500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 72 THE 95% CONFIDENCE INTERVAL IS 67 78
COHO SMOLT (213 HOURS)
COPPER -
FEBRUARY 21, 1982 PROJECT 4 DILUTER A 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 ' 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.2727 0.7500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 72 THE 95% CONFIDENCE INTERVAL IS 66 77 COHO SMOLT (239 HOURS)
COPPER FEBRUARY 21, 1982 PROJEC1 4 DILUTER A 15% TRIMMED SPEARMAH-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 22 22 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.2727 0.8500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 70 THE 95% CONFIDENCE INTERVAL IS 65 75 1
COHO SMOLT (65 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 10% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (69 HOURS)
IINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 10% SPEARMAN-KARBER NOT CALCULABLE COHO SHOLT (89 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 10% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (96 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 10% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (113 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 10% TRIMMED SPEARMAH-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE OR 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 2s 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.1000 0.2000 0.6000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1468 THE 95% CONFIDENCE INTERVAL IS 1125 1916 COHO SMOLT (116 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.1500 0.3000 0.6000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1309 THE 95% CONFIDENCE INTERVAL IS 931 1841
d COHO SMOLT (120 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2000 0.3500 0.6000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1229 THE 95% CONFIDENCE INTERVAL IS 869 1738 COHO SMOLT (138 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2000 0.6000 0.7500 1.0000 THE SPEARMAN KARBER LC50 ESTINATE IS 960 THE 95% CONFIDENCE INTERVAL IS 683 1349 COHO SMOLT (160 HOURS) l ZINC -
l FEBRUARY 27, 1982 PROJECT 4, DILUTER D f
10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 i THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 l THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2000 0.6500 0.8000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 904 THE 95% CONFIDENCE INTERVAL IS 647 1263 COHO SHOLT (186 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 00 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2000 0.7000 0.8000 1.0000 l THE SPEARMAN KARBER LC50 ESTIMATE IS 876 THE 95% CONFIDENCE INTERVAL IS 628 1221 l
- 1 - _ _
COHO SMOLT (210 HOURS)
IINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2000 0.7500 0 8000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 849 THE 95% CONFIDENCE INTERVAL IS 611 1181 i
l I
l
COHO SMOLT (65 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 15% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (69 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 15% TRIMMED SPEARMAN-KARBER ANALYSIS l THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 j THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.0000 0.0000 0.0000 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 2326 )
THE 95% CONFIDENCE INTERVAL IS 2216 2442 -
COHO SMOLT (89 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.0500 0.0500 0.3500 0.9000 THE SPEARMAN KARBER '.C50 ESTIMATE IS 1977 THE 95% CONFIDENCE INIERVAL IS 1673 2335 COHO SMOLT (96 HOURS)
ZINC i FEBRUARY 27, 1982 PROJECT 4, DILUTER D 1
15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WElE 08 386 650 1062 1768 2949
. THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.0500 0.1000 0.4000 0.9000 i
THE SPEARMAN KARBER LC50 ESTIMATE IS 1900 l THE 95% CONFIDENCE INTERVAL IS 1604 2251 l
l
COHO SMOLT (113 HOURS)
ZINC FEBRUARY 27, 1982
> PROJECT 4, DILUTER D 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.1000 0.2000 0.6000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1542 THE 95% CONFIDENCE INTERVAL IS 1272 1870 COHO SMOLT (116 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.1500 0.3000 0.6000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1427 THE 95% CONFIDENCE INTERVAL IS 1150 1772 COHO SMOLT (120 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE OB 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2000 0.3500 0.6000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1328 i THE 95% CONFIDENCE INTERVAL IS 1028 1716 COHO SMOLT (138 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2000 0.6000 0.7500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1008 THE 95% CONFIDENCE INTERVAL IS 792 1283
i l
l l
COHO SMOLT (160 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 15% TRIMMED SPEARMAN-KARBER ANALYSIS r THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20
\
THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2000 0.6500 0.8000 1.0000 l
\
THE SPEARMAN KARBER LC50 ESTIMATE IS 946 l
THE 95% CONFIDENCE INTERVAL IS 752 1189 i
COHO SHOLT (186 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D.
15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2000 0.7000 0.8000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 913 THE 95% CONFIDENCE INTERVAL IS 729 1143 COHO SHOLT (210 HOURS)
ZINC FEBRUARY 27, 1982 PROJECT 4, DILUTER D 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1500 0.2000 0.7500 0.8000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 801 )
THE 95% CONFIDENCE INTERVAL IS 707 1097 !
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COHO SHOLT (24 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.1000 0.9250 THE SPEARMAN KARBER LC50 ESTIMATE IS 55 THE 95% CONFIDENCE INTERVAL IS 53 56 COHO SMOLT (41 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.6750 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 43 THE 95% CONFIDENCE INTERVAL IS 40 46 COHO SM0LT (44 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0250 0.7500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 42 THE 95% CONFIDENCE INTERVAL IS 39 44 COHO SMOLT (48 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0250 0.8500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 40 THE 95% CONFIDENCE INTERVAL IS 39 42
O COHO SMOLT (64 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1000 0.9750 1.0000 1
THE SPEARMAN KARBER LC50 ESTIMATE IS 38 l THE 95% CONFIDENCE INTERVAL IS 38 39 I COHO SHOLT (68 HOURS) i COPPER / ZINC (CDPPER) l FEBRUARY 21, 1982 l PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1000 1.0000 1.0000 l THE SPEARMAN KARBER LC50 ESTIMATE IS 38 THE 95% CONFIDENCE INTERVAL IS 38 39 COHO SMOLT (72 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.1500 1.0000 1.0000 l l THE SPEARMAN KARBER LC50 ESTIMATE IS 38 THE 95% CONFIDENCE INTERVAL IS 36 40 l
C0HO SMOLT (89 HOURS) I l
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.2500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 37 THE 95% CONFIDENCE INTERVAL IS 35 38
COHO SMOLT (92 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 i THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 )
THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.3000 1.0000 1.0000 l THE SPEARMAN KARBER LC50 ESTIMATE IS 36 THE 95% CONFIDENCE INTERVAL IS 34 38 COHO Sh0LT (96 HOURS)
FEBRUARf 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.3250 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 36 THE 95% CONFIDENCE INTERVAL IS 34 38 COHO SHOLT (112 HOURS)
FEBRUARY 21, 1982 ,
PROJECT 4 DILUTER B l 10% TRIMMED SPEARMAN-KARBER ANALYSIS 1 THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 l THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 l THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.3500 1.0000 1.0000 l THE SPEARMAN KARBER LC50 ESTIMATE IS 35 1 THE 95% CONFIDENCE INTERVAL IS 33 37 C0HO SMOLT (117 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.4000 1.0000 1.0000 I
THE SPEARMAN KARBER LC50 ESTIMATE IS 35 THE 95% CONFIDENCE INTERVAL IS 33 37
COHO SMOLT (136 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.5250 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 33 THE 95% CONFIDENCE INTERVAL IS 31 35 l
COHO SMOLT (143 HOURS) i COPPER / ZINC (COPPER) l FEBRUARY 21, 1982 l PROJECT 4 DII. UTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.5500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 33 THE 95% CONFIDENCE INTERVAL IS 31 35 COHO SMOLT (164 HOURS)
COPPER / ZINC (COPPER) i FEBRUARY 21, 1982
( PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.6000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 32 THE 95% CONFIDENCE INTERVAL IS 31 34 COHO SM0LT (172 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.6500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 32 THE 95% CONFIDENCE INTERVAL IS 30 34
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COHO SMOLT (186 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.7000 1.0000 1.0000 ;
1 THE SPEARMAN KARBER LC50 ESTIMATE IS 31 THE 95% CONFIDENCE INTERVAL IS 30 33 l
)
COHO SMOLT (209 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 17 26 33 46 66 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.7250 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 31 THE 95% CONFIDENCE INTERVAL IS 30 33
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, COHO SMOLT (24 HOURS) i COPPER / ZINC (ZINC)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0 0000 0.0000 0.0000 0.0000 0.1000 0.9250 THE SPEARMAN KARBER LC50 ESTIMATE IS 1151 ;
l THE 95% CONFIDENCE INTERVAL IS 1120 1183 )
l COHO SMOLT (41 HOURS)
FEBRUARY 21, 1982 l
PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.6750 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 915 THE 95% CONFIDENCE INTERVAL IS 863 970 l
l COHO SMOLT (44 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0250 0.7500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 886 i
THE 95% CONFIDENCE INTERVAL IS 841 933 l
l 1
COHO SMOLT (48 HOURS) l COPPER / ZINC (ZINC) l l FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS '
THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0250 0.8500 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 855 THE 95% CONFIDENCE INTERVAL IS 825 887
COHO SHOLT (64 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1000 0.9750 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 823 THE 95% CONFIDENCE INTERVAL IS 807 839 COHO SMOLT (68 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 820 THE 95% CONFIDENCE INTERVAL IS 805 834 COHO SMOLT (72 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) ,WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.1500 1.0000 1.0000 I
THE SPEARMAN KARBER LC50 ESTIMATE IS 808 THE 95% CONFIDENCE INTERVAL IS 761 858 i
COHO SHOLT (89 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROP 0RTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.2500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 777 THE 95% CONFIDENCE INTERVAL IS 729 829 l
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l COHO SHOLT (92 HOURS)
COPPER /2INC (Z1NC)
FEBRUARY 21e 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 l THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.3000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 762 i THE 95% CONFIDENCE INTERVAL IS 713 814 COHO SMOLT (96 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.3250 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 754 THE 95% CONFIDENCE INTERVAL IS 705 806 COHO SHOLT (112 HOURS)
FEBRUARY 21, 1982 FROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.3500 1.0000 1 0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 746 THE 95% CONFIDENCE INTERVAL IS 697 798 COHO SMOLT (117 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.4000 1.0000 1 0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 730 THE 95% CONFIDENCE INTERVAL IS 661 7,83
COHO SHOLT (136 HOURS)
FEBRUARY 21, 1982 PROJEC1 4 DILUTER B ,
105 TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK A0 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.5250 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 692 THE 95% CONFIDENCE INTERVAL IS 646 742 COHO Sh0LT (143 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.5500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 685 THE 95% CONFIDENCE INTERVAL IS 639 734 COHO SMOLT (164 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARDER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0250 0.6000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTINATE IS 671 THE 95% CONFIDENCE INTERVAL IS 627 718 COHO SMOLT (172 HOURS)
FEBRUARY 21e 1982 PROJECT 4 DILUTER B 10% TRIMMED SPFARMAN-KARBER ANALYSIS THE DOSES (PARfS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0 0000 0.0250 0.6500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 657 THE 95% CONFIDENCE INTERVAL IS 615 701
COHO SHOLT (186 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE TANK 40 40 40 40 40 40 l THE PROPORTION DEAD I,N 96 HOURS WERE 0.0000 0.0000 0.0250 0.7000 1.0000 1.0000 l
THE SPEARMAN KARBER LC50 ESTIMATE IS 643 THE 95% CONFIDENCE INTERVAL IS 605 684 COHO SHOLT (209 HOURS)
FEBRUARY 21, 1982 PROJECT 4 DILUTER B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 326 480 717 969 1383 THE NUMBER OF FISH IN RESPECTIVE. TANK 40 40 40 40 40 40 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0 0250 0.7250 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 637 THE 95% CONFIDENCE INTERVAL IS 600 676
/
STEELHEAD SMOLT (94 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.0500 0.0500 0.8000 THE SPEARMAN'KARBER LC50 ESTIMATE IS 94 THE 95% CONFIDENCE INTERVAL IS 91 97 STEELHEAD SMOLT (112 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.0500 0.1500 0.8500 THE SPEARMAN KARBER LC50 ESTIMATE IS 92 THE 95% CONFIDENCE INTERVAL IS 89 95 STEELHEAD SMOLT (136 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1000 0.1000 0.1500 0.8500 THE SPEARMAN KARBER LC50 ESTIMATE IS 92 THE 95% CONFIDENCE INTERVAL IS 89 95 I
STEELHEAD SMOLT (143 HOURS)
COPPER FEBRUARY 21,.1982 PROJECT 4 DILUTER A 21% TRIMMED SPEARMAH-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20
'THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1000 0.1500 0.1500 0.8500 THE SPEARMAN KARBER LC50 ESTIMATE IS 92 THE 95% CONFIDENCE INTERVAL IS 89 95
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1 STEELHEAD SMOLT (144 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A l 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1500 0.2000 0.2000 0.8500 ;
l THE SPEARMAN KARBER LC50 ESTIMATE IS 91 l THE 95% CONFIDENCE INTERVAL IS 87 95 STEELhEAD SMOLT (172 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1500 0.2000 0.2500 0.8500 THE SPEARMAN KARBER LC50 ESTIMATE IS 90 THE 95% CONFIDENCE INTERVAL IS 82 98 STEELHEAD SMOLT (186 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 21% TRIHMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPOP. TION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2000 0.2000 0.2500 0.8500 THE SPEARMAN KARBER LC50 ESTIMATE IS 90 THE 95% CONFIDENCE INTERVAL IS 82 98 i
STEELHEAD SMOLT (192 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLIDH) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2500 0.2000 0.2500 0 8500 THE SPEARMAN KARBER LC50 ESTIMATE IS 89 THE 95% CONFIDENCE INTERVAL IS 79 99
STEELHEAD SMOLT (209 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2500 0.2000 0.3000 0.8500 Tile SPEARMAN KARBER LC50 ESTIMATE IS 87 THE 95% CONFIDENCE INTERVAL IS 77 98 STEELHEAD SHOLT (233 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 82 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2500 0.2500 0 3000 0.8500 THE SPEARMAN KARBER LC50 ESTIMATE IS 86 THE 95% CONFIDENCE INTERVAL IS 76 97 STEELHEAD SMOLT (239 HOURS)
COPPER FEBRUARY 21, 1982 PROJECT 4 DILUTER A 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 43 54 63 32 103 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2500 0.2500 0.3500 0.8500 THE SPEARMAN KARBER LC50 ESTIMATE IS 84 THE 95% CONFIDENCE INTERVAL IS 74 95
STEELHEAD SMOLT (25 HOURS) l ZINC FEBRUARY 27, 1983 PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS l THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 I THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 l THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1000 0.6500 0.9500 THE SPEARMAN KARBER LC50 ESTIMATE IS 1586 THE 95% CONFIDENCE INTERVAL IS 1375 1829 STEELHEAD SHOLT (28 HOURS)
ZINC FEBRUARY 27, 1983 i
PROJECT 4 DILUTER D l 10% TRIMMED SPEARMAN-KARBER ANALYSIS l THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.1500 0.8000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1404 l
THE 952 CONFIDENCE INTERVAL IS 1229 1604 STEELHEAD SMOLT (43 HOURS)
ZINC FEBRUARY 27, 1983 PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.2000 0.4000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 1055 j THE 95% CONFIDENCE INTERVAL IS 893 1247 1
1 STEELHEAD SMOLT (45 HOURS) i ZINC FEBRUARY 27, 1983 i PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS i THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 I THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.2500 0.5000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 965 THE 95% CONFIDENCE INTERVAL IS 808 1152
1 1
1 i
- STEELHEAD SM0LT (65 HOURS)
.IINC i
. FEBRUARY 27, 1983 PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.4000 0.8000 1.0000 1.0000 l
THE SPEARMAN KARBER LC50 ESTIMATE IS 720 l
THE 95% CONFIDENCE INTERVAL IS 604 858 STEELHEAD SMOLT (72 HOURS)
ZINC ,
l FEBRUARY 27, 1983 i PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSTS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.4000 0.8500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 702 THE 95% CCNFIDENCE INTERVAL IS 595 827 STEELHEAD SMOLT (89 HOURS)
ZINC FEBRUARY 27, 1983 PROJECT 4 DILUTER D 103 TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.2500 0.5500 0.8500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 489 THE 95% CONFIDENCE INTERVAL IS 301 793 STEELHEAD SMOLT (95 HOURS)
ZINC FEBRUARY 27, 1983 PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 i THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.2500 0.6000 0.8500 1.0000 1.0000 '
THE SPEARMAN KARBER LC50 ESTIMATE IS 473 THE 95% CONFIDENCE INTERVAL IS 292 768
STEELHEAD SMOLT (96 HOURS)
ZINC FEBRUARY 27, 1983 ,
PROJECT 4 DILUTER D '
10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 '
THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.3000 0.6500 0.8500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 406 THE 95% CONFIDENCE INTERVAL IS 239 691 4
STEELHEAD SMOLT (113 HOURS)
ZINC FEBRUARY 27, 1983 PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.3500 0.8000 0.9000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 319 THE 95% CONFIDENCE INTERVAL IS 183 557 STEELHEAD SMOLT (116 HOURS)
ZINC FEBRUARY 27, 1983 PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 20 20 20 20 20 20 THE NUMBER OF FISH IN RESPECTIVE TANK THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.3500 0.9000 0.9000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 300 THE 95% CONFIDENCE INTERVAL IS 160 562 STEELHEAD SMOLT (138 HOURS)
ZINC FEBRUARY 27, 1983 PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 20 20 20 20 20 20 THE NUMBER OF FISH IN RESPECTIVE TANK THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5500 0.9000 0.9000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 177 THE 95% CONFIDENCE INTERVAL IS 94 333
STEELHEAD SMOLT (160 HOURS)
ZINC FEBRUARY 27, 1983 PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.5500 0.9000 0.9500 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 177 -
THE 95% CONFIDENCE INTERVAL IS 94 333 STEELHEAD SMOLT (186 HOURS)
ZINC FEBRUARY 27, 1983 PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.8000 0.950061001.0 1.0000 0.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 89 THE 95% CONFIDENCE INTERVAL IS 55 146 STEELHEAD SMOLT (210 HOURS)
ZINC FEBRUARY 27, 1983 PROJECT 4 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 386 650 1062 1768 2 949 THE NUMBER OF FISH'IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.8000 1.0000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 89 THE 95% CONFIDENCE INTERVAL IS 55 144
+
a a PROJECT 5 9
COHO SMOLT (131 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 10% SPEARNAN-KARBER NOT CALCULABLE COHO SHOLT (159 HOURS)
COPPER MARCH 17, 1982 ,
PROJECT 5, DILUTERS A & B 10% SPEARMAN-KARBER NOT CALCULABLE COHO SHOLT (179 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A 1 B 10% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (202 HOURS)
COPPER MARCH 17, 1982 PROJECT 5e DILUTERS A & B 10% SPEARMAN-KARBER NOT CALCULABLE COHO SHOLT (257 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 10% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (276 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 10% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (300 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A 1 B 10% SPEARMAN-KARBER NOT CALCULABLE
COHO SMOLT (348 HOURS)
COPPER MARCH 17, 1982 l PROJECT 5, DILUTERS A 1 B ;
10% SPEARMAH-KARBER NOT CALCULABLE COHO SMOLT (373 HOURS)
COPPER l MARCH 17, 1982 PROJECT 5, DILUTERS A & B 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 l
THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.6000 0.9333 THE SPEARMAN KARBER LC50 ESTIMATE IS 69 THE 95% CONFIDENCE INTERVAL IS 64 74 COHO SMOLT (396 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B l 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLI0d) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.6333 0.9667 l
THE SPEARMAN KARBER LC50 ESTIMATE IS 68
, THE 95% CONFIDENCE INTERVAL IS 64 73 l
l COHO SMOLT (467 HOURS) '
! MARCH 17, 1982 l
PROJECT Se DILUTERS A & B 10% TRIMMED SPEARMAN-KARBER ANALYSIS l THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 l j THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 )
1 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.7000 0.9667 THE SPEARMAN KARBER LC50 ESTIMATE IS 67 i THE 95% CONFIDENCE INTERVAL IS 62 71 l
I
l COHO SMOLT (696 HOURS)
COPPER MARCH 17, 1982 )
PROJECT Se DILUTERS A 1 B 10% TRIMMED SPEARMAN-KARBER ANALYSIS ,
THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE MUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.7667 0.9667 THE SPEARMAN KARBER LC50 ESTIMATE IS 65 THE 95% CONFIDENCE INTERVAL IS 61 70 I
COHO SMOLT (131 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 15% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (159 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 15% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (179 HOURS)
COPPER MARCH 17, 1902 -
PROJECT 5, DILUTERS A & B 15% SPEARMAN-KARBER NOT CALCULABLE COHO SMOLT (202 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A 8 B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0667 0.5000 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 74 THE 95% CONFIDENCE INTERVAL IS 68 80 l COHO SMOLT (257 HOURS) l COPPER I MARCH 17, 1982 -
PROJECT 5, DILUTERS A 1 B l 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1000 0.5000 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 73 THE 95% CONFIDENCE INTERVAL IS 68 79
COHO SMOLT (276 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1000 0.5000 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 73 THE 95% CONFIDENCE INTERVAL IS 68 79 COHO SMOLT (300 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.5000 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 73 THE 95% CONFIDENCE INTERVAL IS 67 79 COHO SMOLT (348 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.5667 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 71 i
THE 95% CONFIDENCE INTERVAL IS 65 77 C0HO SMOLT (373 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.6000 0.9333 THE SPEARMAN KARBER LC50 ESTIMATE IS 70 THE 95% CONFIDENCE INTERVAL IS 64 75
I l
COHO SMOLT (396 HOURS)
COPPER l
MARCH 17, 1982 l PROJECT 5, DILUTERS A & B l
15% TRIMMED SPEARMAN-KARBER ANALYSIS
- l. THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 l THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.6333 0.9667 THE SPEARMAN KARBER LC50 ESTIMATE IS 69 THE 95% CONFIDENCE INTERVAL IS 64 74 ;
1
, COHG SMOLT (467 HOURS)
COPPER l MARCH 17, 1982 l PROJECT 5, DILUTERS A 8 8 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24' 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.7000 0.9667 THE SPEARMAN KARBER LCS0 ESTIMATE IS 67 THE 95% CONFIDENCE INTERVAL IS 62 72 l
! COHO SMOLT (696 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B
( 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 i THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.7667 0.9667 THE SPEARMAN KARBER LC50 ESTIMATE IS 65 l THE 95% CONFIDENCE INTERVAL IS 61 70 l
" C0HO SMOLT (131 HOURS)
COPPER MARCH 17, 1982 DILUTERS A & B 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0333 0.3333 0.8000 THE SPEARMAN KARBER LC50 ESTINATE IS 81 THE 95% CONFIDENCE INTERVAL IS 72 90 COHO SMOLT (159 HOURS)
COPPER -
MARCH 17, 1982 DILUTERS A & B 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0333 0.3667 0.8333 THE SPEARMAN KARBER LC50 ESTIMATE IS 79 THE 95% CONFIDENCE INTERVAL IS 72 88 COHO SMOLT (179 HOURS)
COPPER MARCH 17, 1982 DILUTERS A & B 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0667 0.3667 0.8333 THE SPEARMAN KARBER LC50 ESTIMATE IS 79 THE 95% CONFIDENCE INTERVAL IS 72 37 COHO SMOLT (202 HOURS)
COPPER MARCH 17, 1982 DILUTERS A 1 B 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS FER BILLIGH) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0667 0.5000 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 75 THE 95% CONFIDENCE INTERVAL IS 68 82
l COHO SMOLT (257 HOURS)
COPPER >
MARCH 17, 1982 1 DILUTERS A & B 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1000 0.5000 0.9667 THE SPEARMAN KARBER LC50 ESTIMATE IS 74 THE 95% CONFIDENCE INTERVAL IS 67 81 l
COHO SMOLT (276 HOURS)
COPPER-MARCH 17, 1982 DILUTERS A 8 B 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1000 0.5000 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 74 THE 95% CONFIDENCE INTERVAL IS 67 81 COHO SMOLT (300 HOURS)
COPPER MARCH 17, 1982 i DILUTERS A 8 B l 21% TRIMMED SPEARMAN-KARBER ANALYSIS l THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 70 89 i
THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 i
THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.5000 0.8667 I
THE SPEARMAN KARBER LC50 ESTIMATE IS 74 THE 95% CONFIDENCE INTERVAL IS 67 81 i
COHO SMOLT (348 HOURS) l COPPER MARCH 17, 1982 DILUTERS A & B 21% TRIMMED SPEARMAN-KARBER ANALYSIS
! THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30. 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.5667 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 71 THE 95% CONFIDENCE INTERVAL IS 65 78
COHO SMOLT (373 HOURS)
COPPER MARCH 17, 1982 DILUTERS A & B 21% TkIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.6000 0.9333 THE SPEARMAN KARBER LC50 ESTIMATE IS 70 .
THE 95% CONFIDENCE INTERVAL IS 64 77 COHdSMOLT(396 HOURS)
COPPER MARCH 17, 1982 DILUTERS A & B 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.6333 0.9667 THE SPEARMAN KARBER LC50 ESTIMATE IS 69 THE 95% CONFIDENCE INTERVAL IS 63 75 COHO SHOLT (467 HOURS)
COPPER MARCH 17, 1982 DILUTERS A & B 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.7000 0.9667 i
THE SPEARMAN KARBER LC50 ESTIMATE IS 67 THE 95% CONFIDENCE INTERVAL IS 62 73 COHO SMOLT (496 HOURS)
COPPER MARCH 17, 1982 DILUTERS A & B 21% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1333 0.7667 0.9667 THE SPEARMAN KARBER LC50 ESTIMATE IS 65 THE 95% CONFIDENCE INTERVAL IS 61 70
^
I I
C0!i9 SMOLT (696 HOURS) l l ZINC l MARCH 18, 1992 l DILUTERS C & D l l 30% TRIMMED SPEARMAN-KARBER ANALYSIS '
'THE DOSES (PARTS PER BILLION) WERE 2.20 3.26 6.12 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 THT PROPORTION CCAD IN 96 HOURS WERE 0.0000 0.2000 0.7333 l
THE SPEARMAN KARBER LC50 ESTIMATE IS 4.65 THE 95% CONFIDENCE INTERVAL IS 4.07 5.30 l
l l
l l
l
\
l
)
I i
r i
STEELHEAD SMOLT (257 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 15% SPEARMAN-KARBER NOT CALCULABLE ,
STEELHEAD SHOLT (276 HOURS) .
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 15% SPEARMAN-KARBER NOT CALCULABLE STEELHEAD SMOLT (300 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 15% SPEARMAN-KARBER NOT CALCULABLE STEELHEAD GMOLT (323 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A 1 B 15% SPEARMAN-KARBER NOT CALCULABLE O
STEELHEAD SMOLT (396 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2000 0.4000 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 74 THE 95% CONFIDENCE INTERVAL IS 65 84 STEELHEAD SMOLT (467 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A 1 B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2000 0.4667 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 72 THE 951 CONFIDENCE INTERVAL IS 63 82
STEELHEAD SMOLT (696 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A 8 8 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2667 0.4667 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 70 ,
THE 95% CONFIDENCE INTERVAL IS 61 80 STEELHEAD SMOLT (960 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER 07 FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2667 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 58 THE 95% CONFIDENCE INTERVAL IS 53 64
l l
)
STEELHEAD SMOLT (257 HOURS) l COPPER MARCH 17, 1982 ,
PROJECT 5, DILUTERS A 8 B j 25% SPEARMAH-KARBER NOT CALCULABLE l STEELHEAD SHOLT (276 HOURS) l COPPER MARCH 17, 1982 I PROJECT 5e DILUTERS A 1 B.
25% SPEARMAN-KARBER NOT CALCULABLE l STEELHEAD SHOLT (300 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 25% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2000 0.3333 0.8000 THE SPEARMAN KARBER LC50 ESTIMATE IS 80 i THE 95% CONFIDENCE INTERVAL IS 69 94 STEELHEAD SHOLT (323 HOURS)
COPPER MARCH 17, 1982 ,
PROJECT 5, DILUTERS A & B 25% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2000 0.4000 0.8000 THE SPEARMAN KARBER LC50 ESTIMATE IS 77 THE 95% CONFIDENCE INTERVAL IS 66 91 f
STEELHEAD SMOLT (396 HOURS)
COPPER MARCH 17, 1982 PROJECT 5, DILUTERS A & B 25% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2000 0.4000 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 77 THE 95% CONFIDENCE INTERVAL IS 66 90
STEELHEAD SMOLT (467 HOURS)
COPPER MARCH 17, 1982 l PROJECT 5, DILUTERS A & B 25% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51- 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2000 0.4667 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 74 THE 95% CONFIDENCE INTERVAL IS 64 87 STEELHEAD SMOLT (696 HOURS) l
. COPPER MARCH 17, 1982 I PROJECT 5, DILUTERS A & B 25% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2667 0.4667 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 73 THE 95% CONFIDENCE INTERVAL IS 61 86
STEELHEAD SMOLT (257 HOURS)
COPPER MARCH 17, 1982 DILUTERS A 8 B 30% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.1333 0.3333 0.7333 THE SPEARMAN KARBER LC50 ESTIMATE IS 82 THE 95% CONFIDENCE INTERVAL IS 63 107 STEELHEAD SMOLT (276 HGURS)
COPPER MARCH 17, 1982 DILUTERS A 1 B '
30% TRIhMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2000 0.3333 0.7333 THE SPEARMAN KARBER LC50 ESTIMATE IS 82 THE 95% CONFIDENCE INTERVAL IS 64 105 STEELHEAD SHOLT (300 HOURS) ,
COPPER MARCH 17, 1982 DILUTERS A & B 30% TRIMMED SPEARMAN-KARBER ANALYSIS -
THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2000 0.3333 0.8000 THE SPEARMAN KARBER LC50 ESTIMATE IS 81 THE 95% CONFIDENCE INTERVAL IS 64 104 4
STEELHEAD SMOLT (323 HOURS)
COPPER MARCH 17, 1982 DILUTERS A 1 B 30% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2000 0.4000 0.8000 THESPEARMANKARBERLC50ESTIMATEIS 79 THE 95% CONFIDENCE INTERVAL IS 64 97
STEELHEAD SHOLT (396 HOURS)
COPPER MARCH 17, 1982 1 DILUTERS A 8 8 30% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2000 0.4000 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 78 THE 95% CONFIDENCE INTERVAL IS 64 96 STEELHEAD SMOLT (467 HOURS)
COPPER MARCH 17, 1982 DILUTERS A & B 30% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2000 0.4667 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 75 THE 95% CONFIDENCE INTERVAL IS 62 92 STEELHEAD SMOLT (696 HOURS)
COPPER MARCH 17, 1982 DILUTERS A & B 30% TRIMMED SPEARMAN-KARBER ANALYSIS i THE DOSES (PARTS PER BILLION) WERE 03 24 45 51 78 89 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 4 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.2667 0.4667 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 74 THE 95% CONFIDENCE INTERVAL IS 61 90 l
Siu 'AD SMOLT (179 HOURS)
ZINL PROJECT 5, DILUTERS C 1 D MARCH 18, 1982 10% SPEARMAN-KARBER NOT CALCULABLE ,
STEELHEAD SMOLT (202 HOURS)
ZINC PROJECT 5, DILUTERS C 1 D MARCH 18, 1982 10% SPEARMAN-KARBER NOT CALCULABLE STEELHEAD SMOLT (227 HOURS)
ZINC PROJECT 3, DILUTERS C 1 D MARCH 18, 1982 10% SPEARMAN-KARBER NOT CALCULABLE-STEELHEAD SMOLT (300 HOURS)
ZINC PROJECT 5, DILUTERS C 1 D MARCH 18, 1982 10% SPEARMAN-KARBER NOT CALCULABLE STEELHEAD SMOLT (348 HOURS)
ZINC PROJECT 5, DILUTERS C 1 D HARCH 18, 1982 10% SPEARMAN-KARBER NOT CALCULABLE STEELHEAD SMOLT (348 HOURS)
ZINC PROJECT 5, DILUTERS C 8 D MARCH 18, 1982 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DO3ES (PARTS PER BILLION) WERE 59 66 93 119 179 220 326 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6000 0.5333 0.4667 0.8667 0.9333 0.9333 THE SPEARMAN KARBER LC50 ESTIMATE IS 120 THE 95% CONFIDENCE INTERVAL IS 100 145
STEELHEAD SMOLT (179 HOURS)
ZINC PROJECT 5, DILUTERS C 8 D NARCH 18, 1982 15% SPEARMAN-KARBER NOT CALCULABLE STEELHEAD SMOLT (202 HOURS)
ZINC PROJECT 5, DILUTERS C & D NARCH 18, 1982 15% SPEARNAN-KARBER NOT CALCULABLE I I
l STEELHEAD SMOLT (227 HOURS)
ZINC PROJECT 5, DILUTERS C 1 D MARCH 18, 1982 15% TRINMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 59 66 93 119 179 220 326 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 15 15 THE PROPORTIDN DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6000 0.5333 0.4667 0.8000 0.8000 0.8667 THE SPEARMAN KARBER LC50 ESTINATE IS 126 THE 95% CONFIDENCE INTERVAL IS 97 165 STEELHEAD SMOLT (300 HOURS) i IINC PROJECT 5, DILUTERS C 8 D .
MARCH 18, 1982 15% TRIMMED SPEARMAN-KARBER ANALYSIS
! THE DOSES (PARTS PER LILLION) WERE 59 66 93 119 179 220 326 612
( THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 15 15 l
THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6000 0.5333 0.4667 0.8667 0.8667 0.3667 THE SPEARMAN KARBER LC50 ESTIMATE IS 119 l
THE 95% CONFIDENCE INTERVAL IS 98 146
STEELHEAD SMOLT (348 HOURS)
ZINC PROJECT 5: DILUTERS C & D MARCH 18, 1982 15% iRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLIDN) WERE 59 66 93 119 179 220 326 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6000 0.5333 0.4667 0.8667 0.9333 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 119 THE 95% CONFIDENCE INTERVAL IS 98 146 STEELHEAD SMOLT (348 HOURS)
ZINC PROJECT 5, DILUTERS C & D MARCH 18, 1982 15% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLIDN) WERE 59 66 93 119 179 220 326 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 15 15 THE PROPORTIDN DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6000 0.5333 0.4667 0.8667 0.9333 0.9333 THE SPEARMAN KARBER LC50 ESTIMATE IS 119 THE 95% CONFIDENCE INTERVAL IS 98 146 t
O
STEELHEAD SMOLT (179 HOURS)
ZINC PROJECT 5, DILUTERS C 1 D MARCH 18, 1982 30% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 59 66 93 119 179 220 326 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6000 0.5333 0.4667 0.7333 0.7333 0.6667 THE SPEARNAN KARBER LC50 ESTIMATE IS 120 THE 95% CONFIDENCE INTERVAL IS 85 169 STEELHEAD SMOLT (202 HOURS) l ZINC PROJECT 5 DILUTERS C 1 D MARCH 18e 1982 .
30% TRIMMED SPEARMAN-KARBER ANALYSIS l THE DOSES (PARTS PER BILLION) WERE 59 66 93 117 179 220 326 612 )
THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 15 15 THE PROPDRTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6000 0.5333 0.4667 0.8000 0.0000 0.7333 THE SPEARMAN KARBER LC50 ESTIMATE IS 119 THE 95% CONFIDENCE INTERVAL IS 85 166 i STEELHEAD SHOLT (227 HOURS)
ZINC PROJECT 5, DILUTERS C 1 D MARCH 18, 1982 30% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 59 66 93 119 179 220 326 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6000 0.5333 0.4667 0.8000 0.8000 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 118 THE 95% CONFIDENCE INTERVAL IS 85 165 STEELHEAD SMOLT (300 HOURS)
ZINC PROJECT 5, DILUTERS C 1 D l MARCH 18, 1982 I
( 30% TRIMMED SPEARMAN-KARBER ANALYSIS l THE DOSES (PARTS PER BILLION) WERE 59 66 93 119 179 220 326 612 l THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6000 0.5333 0.4667 0.8667 0.8667 0.8667 i
THE SPEARMAN KARBER LC50 ESTIMATE IS 118 THE 95% CONFIDENCE INTERVAL IS 85 163 i
L
STEELHEAD SMOLT (348 HOURS)
ZINC PROJECT 5, DILUTERS C 1 D MARCH 18, 1982 30% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE . 59 66 93 119 179 220 326 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 15 15 15 15 15 THE PROPDRTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6000 0.5333 0.4667 0.8667 0.9333 0.8667 THE SPEARMAN KARBER LC50 ESTIMATE IS 118 THE 95% CDNFIDENCE INTERVAL IS 85 163 STEELHEAD SMOLT (348 HOURS)
ZINC PROJECT 5, DILUTERS C & D NARCH 18, 1982 30% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 59 66 93 119 179 220 326 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 1S 15 15 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6000 0.5333 0.4667 0.8667 0.9333 0.9333 THE SPEARMAN KARBER LC50 ESTIMATE IS 118 THE 95% CONFIDENCE INTERVAL IS 85 163 r
- 6 I
i I
PROJECT 6 .
p 9
A
COHO FRY (171 HOURS)
COPPER MAY 28 - JUNE 1, 1982 PROJECT 4, DILUTER A 103. TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 61 THE 95% CONFIDENCE INTERVAL IS 60 63 COHO FRY (194 HOURS) '
COPPER MAY 28 - JUNE 1, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 61 THE 95% CONFIDENCE INTERVAL IS 57 65 C0HO FRY (242 HOURS)
COPPER MAY 28 - JUNE 1, 1982 PROJECT 4, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0333 0.1667 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 60 THE 95% CONFIDENCE INTERVAL IS 56 64
COHO FRY (26 HOURS)
COPPER MAY 28 - JUNE 1, 1982 PROJECT 6, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS -
THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0667 0.7667 1.0000 1 0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 66 THE 95% CONFIDENCE INTERVAL IS 62 70 COHO FRY (34 HOURS)
COPPER MAY 28 - JUNE 1, 1982 PROJECT 6, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0667 0.8667 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 64 THE 95% CONFIDENCE INTERVAL IS 61 67 COHO FRY (48 HOURS)
COPPER MAY 28 JUNE 1, 1982 PROJECT 6, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 j THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0667 0.9333 1.0000 1 0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 63 THE 95% CONFIDENCE INTERVAL IS 61 65
)
COHO FRY (105 HOURS)
COPPER MAY 28 - JUNE 1, 1982 PROJECT 6, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 30 30 30 30 30 30 THE NUMBER OF FISH IN RESPECTIVE TANK THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0667 0.9667 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 62 THE 95% CONFIDENCE INTERVAL IS 61 64
COHO SHOLT (26 HOURS)
COPPER MAY 28, 1982 PROJECT 6, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0500 0.9000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 63 THE 95% CONFIDENCE INTERVAL IS 61 66 COHO SMOLT (73 HOURS)
COPPER MAY 28, 1982 PROJECT 6, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.0500 0.9000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 63 THE 95% CONFIDENCE INTERVAL IS 61 66 COHO SMOLT (81 HOURS)
COFr :
MAY 28, 1982 PROJECT 6 DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0500 0.0500 0.0500 0.9000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 63 THE 95% CONFIDENCE INTERVAL IS 61 66 COHO SMOLT (171 HOURS)
COPPER MAY 28, 1982 PROJECT 6, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 THE NUMBER OF FISH IN RESPECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0500 0.0500 0.1000 0.9000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 63 THE 95% CONFIDENCE INTERVAL IS 60 66
COHO SMOLT (194 HOURS)
COPPER MAY 28, 1982 PROJECT 6, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 51 77 97 149 THE NUMBER OF FISH IN RESFECTIVE TANK 20 20 20 20 20 20 THE PROPORTION DEAD IN 96 HOURS WERE 0.0500 0.0500 0.1000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 61 THE 95% CONFIDENCE INTERVAL IS 59 63
COHO FRY (10 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLIOW) WERE 02 33 49 83 143 238 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.1333 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 177 THE 95% CONFIDENCE INTERVAL IS 159 197 COHO FRY (26 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 238 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.2333 0.9667 1.0000 1 0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 59 THE 95% CONFIDENCE INTERVAL IS 53 65 COHO FRY (34 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 238 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.2667 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 57 THE 95% CONFIDENCE INTERVAL IS 52 63 l.
COHO FRY (48 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 238 THE NUMBER OF FISH IN RESPECTIVE TANK 30' 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.3000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 56 THE 95% CONFIDENCE INTERVAL IS 51 62
)
l 1
l COHO FRY (97 HOURS) i COPPER / ZINC (COPPER)
MAY 28, 1982 i PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 238 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.3333 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 55 THE 95% CONFIDENCE INTERVAL IS 50 61 COHO FRY (123 HOURS)
MAY 28, 1982 PROJECT 6 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 238 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.4000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 53 THE 95% CONFIDENCE INTERVAL IS 48 59 COHO FRY (147 HOURS) l COPPER / ZINC (COPPER)
MAY 28, 1982 l PROJECT 6, DILUTER D i
l 10% TRIMMED SPEARMAN-KARBER ANALYSIS
( THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 238 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0333 0.4333 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 52 THE 95% CONFIDENCE INTERVAL IS 47 58 i
COHO FRY (171 HOURS) )
MAY 28, 1982 PROJECT 6, DILUTER D l 10% TRIMMED SPEARMAN-KARBER ANALYSIS l
THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 238 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0333 0.5000 1.0000 1.0000 1 0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 50 THE 95% CONFIDENCE INTERVAL IS 45 56
C0HO FRY (194 HOURS)
MAY 28, 1982 PROJECT 6 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 238 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0333 0.6000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 47 THE 95% CONFIDENCE INTERVAL IS 43 52 E
N
C0HO FRY (10 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 i THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.1333 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 458 THE 95% CONFIDENCE INTERVAL IS 416 503
/
COHO FRY (26 HOURS)
MAY 28, 1982
, )
PROJECT 6, DILUTER D '
10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.2333 0.9667 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 171 THE 95% CONFIDENCE INTERVAL IS 154 189 COHO FRY (34 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIM.MED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612
- THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30
! THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.2667 1.0000 1.0000 1.0000 l
THE SPEARMAN KARBER LC50 ESTIMATE IS 166 THE 95% CONFIDENCE INTERVAL IS 150 184 l
COHO FRY (48 HOURS)
, MAY 28, 1982 '
l PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.3000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 163 THE 95% CONFIDENCE INTERVAL IS 147 181
COHO FRY (97 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.3333 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 160 ,
THE 95% CONFIDENCE INTERVAL IS 144 178 C0HO FRY (123 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.4000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 155 THE 95% CONFIDENCE INTERVAL IS 139 172 COHO FRY (147 HOURS)
MAY 28, 1992 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0333 0.4333 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 151 THE 95% CONFIDENCE INTERVAL IS 135 168 COHO FRY (171 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0333 0.5000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 145 THE 95% CONFIDENCE INTERVAL IS 130 162
COHO FRY (194 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0333 0.6000 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 137 THE 95% CONFIDENCE INTERVAL IS 123 152 I
\
l
S STEELHEAD SMOLT (10 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 2.38 THE HUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.2143 0.2667 0.9333 THE SPEARMAN KARBER LC50 ESTIMATE IS 154 THE 95% CONFIDENCE INTERVAL IS 126 189 STEELHEAD SMOLT (26 HOURS)
MAY 28, 1982 -
PROJECT 6, DILUTER D
' 102 TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 2.38 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.9286 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 62 THE 95% CONFIDENCE INTERVAL IS 55 71 STEELHEAD SMOLT (48 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 2.38 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IP 96 HOURS WERE 0.0000 0.0000 0.3333 0.9286 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 56 THE 95% CONFIDENCE INTERVAL IS 48 65 STEELHEAD SMOLT (59 HOURS) s COPPER / ZINC (COPPER)
MAY 28e 1982 PROJECT 6 DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 2.38 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROP 0RTION DEAD IN 96 HOURS WERE 0.0000 0.0667 0.3333 0.9286 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 56 THE 95% CONFIDENCE INTERVAL IS 48 65
l L
STEELHEAD SHOLT (73 HOURS) l COPPER / ZINC (CDPPER) -
l MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS l THE DOSES (PARTS PER BILLION) WERE ' 02 33 49 83 143 2.38 l THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 4 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.3333 0.9286 1.0000 1.0000 )
l THE SPEARMAN KARBER LC50 ESTIMATE IS 54 l THE 95% CONFIDENCE INTERVAL IS 43 68 i
STEELHEAD SMOLT (81 HOURS) j COPPER / ZINC (COPPER)
MAY 28, 1982 l
PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 2.38 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.4000 0.9286 1.0000 1.0000 l THE SPEARMAN KARBER LC50 ESTIMATE IS 52 THE 95% CONFIDENCE INTERVAL IS 41 66 STEELHEAD SMOLT (96 HOURS)
MAY 28, 1982 l
PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 2.38 I THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6667 0.9286 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 45 THE 95% CONFIDENCE INTERVAL IS 35 56 STEELHEAD SMOLT (105 HOURS) l COPPER / ZINC (COPPER)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS l
THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 2.38 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6667 1.0000 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 44 THE 95% CONFIDENCE INTERVAL IS 35 55
STEELHEAD SMOLT (147 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 33 49 83 143 2.38 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.7333 1.0000,1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 42 THE 95% CONFIDENCE INTERVAL IS 34 53 4
t e-- -.
~
l I
N STEELHEAD SMOLT (10 HOURS) 1 COPPER / ZINC (ZINC)
MAY 28, 1982 PROJECT 6, DILUTER D l 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 1 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.2143 0.2667 0.9333 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 405 THE 95% CONFIDENCE INTERVAL IS 338 486 STEELHEAD SHOLT (26 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1333 0.9286 1.0000 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 181 THE 95% CONFIDENCE INTERVAL IS 158 208 STEELHEAD SMOLT (48 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15
! THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.3333 0.9286 1.0000 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 163 THE 95% CONFIDENCE INTERVAL IS 140 190 STEELHEAD SMOLT (59 HOURS) ,
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS I THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 l THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0667 0.3333 0.9286 1.0000 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 161 l THE 95% CONFIDENCE INTERVAL IS 138 188 l
STEELHEAD SMOLT (73 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORfl0N DEAD IN 96 HOURS WERE 0.0000 0.1333 0.3333 0.9286 1.0000 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 156 THE 95% CONFIDENCE INTERVAL IS 125 195 STEELHEAD SMOLT (81 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.4000 0.9286 1.0000 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 150 THE 95% CONFIDENCE INTERVAL IS 119 189 STEELHEAD SMOLT (96 HOURS) ,
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6667 0.9286 1.0000 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 128 THE 95% CONFIDENCE INTERVAL IS 102 160 STEELHEAD SMOLT (105 HOURS)
MAY 28, 1982 PROJECT 6, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER.0F FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.6667 1.0000 1.0000 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 127 THE 95% CONFIDENCE INTERVAL IS 102 157
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STEELHEAD SMOLT (147 HOURS) 1 I
HAY 28, 1982 PROJECT 6 DILUTER D '
10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 90 147 232 371 612 THE NUMBER OF FISH IN RESPECTIVE TANK 15 15 15 14 15 15 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1333 0.7333 1.0000 1.0000 1.0000 0.0 l THE SPEARMAN KARBER LC50 ESTIMATE IS 122 THE 95% CONFIDENCE INTERVAL IS 99 151 i e
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PROJECT 6A
C"0HO FRY (25 HOURS)
COPPER (ERRATIC DILUTER)
MAY 5, 1982 PROJECT 6A, DILUTER A l 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 38 48 66 80 96 THE NUMBER OF FISH IN RESPECTIVE TANK 30 33 30 30 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0303 0.0000 0.1667 0.9655 THE SPEARMAN KARBER LC50 ESTIMATE IS 84 THE 95% CONFIDENCE INTERVAL IS 83 89 COHO FRY (41 HOURS)
COPPER (ERRATIC DILUTER)
MAY 5, 1982 PROJECT 6A, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 38 48 66 80 96 THE NUMBER OF FISH IN RESPECTIVE TANK 30 33 30 30 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0333 0.0303 0.0000 0.1667 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 86 THE 95% CONFIDENCE INTERVAL IS 83 89 COHO FRY (66 HOURS)
COPPER (ERRATIC DILUTER)
MAY 5, 1982 PROJECT 6A, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS i
THE DOSES (PARTS PER BILLION) WERE 02 38 48 66 80 96 THE NUMBER OF FISH IN RESPECTIVE TANK 30 33 30 30 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0333 0.0606 0.0000 0.1667 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 86 THE 95% CONFIDENCE INTERVAL IS 83 89 COHO FRY (114 HOURS)
COPPER (ERRATIC DILUTER)
MAY 5, 1982 PROJECT 6A, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 38 48 66 80 96 THE NUMBER OF FISH IN RESPECTIVE TANK 30 33 30 30 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0667 0.0606 0.0000 0.1667 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 86 THE 95% CONFIDENCE INTERVAL IS 83 89
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COHO FRY (138 HOURS) l COPPER (ERRATIC DILUTER) l MAY 5, 1982 l PROJECT 6A, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS
. THE DOSES (PARTS PER BILLION) UERE 02 38 48 66 80 96 THE NUMBER OF FISH IN RESPECTIVE TANK 30 33 30 30 30 29 THE PROP 0RTION DEAD IN 96 HOURS WERE 0.0000 0.0667 0.0909 0.0000 0.2000 1.0000 l THE SPEARMAN KARBER LC50 ESTIMATE IS 85 THE 95% CONFIDENCE INTERVAL IS 82 88 COHO FRY (162 HOURS)
COPPER (ERRATIC DILUTER)
MAY 5, 1982 PROJECT 6A, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 38 48 66 80 96 THE NUMBER OF FISH IN RESPECTIVE TANK 30 33 30 30 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0667 0.0909 0.0667 0.3000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 83 THE 95% CONFIDENCE INTERVAL IS 80 86 COHO FRY (185 HOURS)
COPPER (ERRATIC DILUTER)
MAY 5, 1982 PROJECT 6A, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 38 48 66 80 96
- THE NUMBER OF FISH IN RESPECTIVE TANK 30 33 30 ' 30 30 29
- THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.0909 0.0667 0.3333 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 82 THE 95% CONFIDENCE INTERVAL IS 79 86 l
COHO FRY (233 HOURS)
COPPER (ERRATIC DILUTER)
MAY 5, 1982 PROJECT 6A, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 38 48 66 80 96 J
THE NUMBER OF FISH IN RESPECTIVE TANK 30 33 30 30 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.1515 0.0667 0.4000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 80 THE 95% CONFIDENCE INTERVAL IS 75 86 i
i C3HO FRY (258 HOURS)
COPPER (ERRATIC DILUTER)
MAY 5, 1982 PROJECT 6A, DILUTER A 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 39 48 66 80 96 THE NUMBER OF FISH IN RESPECTIVE TANK 30 33 30 30 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.2727 0.0667 0.4333 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 77 THE 95% CONFIDENCE INTERVAL IS 71 83 r w -r -- -y-,e- - - w-._ - , - -,e-w + -- , ,, - - - ---.e .- rr-- , - -m - y
1 COHO SMOLT (209 HOURS) I COPPER (ERRATIC DILUTER) -
MAY 5, 1982 PROJECT 6A, DILUTER A 11% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 38 48 66 80 96 .
THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 THE PROPORTION DEAD IN 96 HOURS WERE 0.1000 0.0000 0.1000 0.1000 0.3000 0.9000 THE SPEARMAN KARBER LC50 ESTIMATE IS 84 THE 95% CONFIDENCE INTERVAL IS 78 90 9
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COHO SMOLT (18 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.8000 1.0000, THE SPEARNAN KARBER LC50 ESTIMATE IS 81 THE 95% CONFIDENCE INTERVAL IS 77 86 l
COHO SMOLT (25 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.9000 1.0000_
THE SPEARMAN KARBER LC50 ESTIMATE IS 79 THE 95% CONFIDENCE INTERVAL IS 77 82
)
i COHO SMOLT (138 HOURS)
MAY 5, 1982 l
PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS )
THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 !
0.0000 0.0000 0.0000 0.1000 0.9000 1.0000 THE PROPORTION DEAD IN 96 HOURS WERE THE SPEARMAN KARBER LC50 ESTIMATE IS 78 THE 95% CONFIDENCE INTERVAL IS 76 82 COHO SMOLT (209 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.2000 0.9000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 77 THE 95% CONFIDENCE INTERVAL IS 70 84
COHO SMOLT (233 HOURS)
MAY 5, 1982 l PROJECT 4A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.1000 0.2000 0.9000 1.0000 1
! THE SPEARMAN KARBER LC50 ESTIMATE IS 76 -
l THE 95% CONFIDENCE INTERVAL IS 69 83 i
i COHO SMOLT (258 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS i THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 l THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 l THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.1000 0.2000 0.9000 1.0000 l THE SPEARMAN KARBER LC50 ESTIMATE IS 74
- THE 95% CONFIDENCE INTERVAL IS 69 83 l
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C0HO SMOLT (18 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 THE PROP 0RTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.8000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 174 THE 95% CONFIDENCE INTERVAL IS 165 184 COHO SMOLT (25 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 THE PROPORTION DEAD IN 96 HOURS WERE 4.0000 0.0000 0.0000 0.0000 0.9000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 171 THE 95% CONFIDENCE INTERVAL IS 167 175 CCHO SMOLT (138 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS .
THE DGSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER OF FISH IN RESFECTIVE TANK 10 10 10 10 10 10 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1000 0.9000 1.0000
.THE SPEARMAN KARBER LC50 ESTIMATE IS 169 THE 95% CONFIDENCE INTERVAL IS 164 175 COHO SMOLT (209 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS.
THE DOSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.2000 0.9000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 166 THE 95% CONFIDENCE INTERVAL IS 156 178
COHO SMOLT (233 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0 1000 0.2000 0.9000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 166 THE 95% CONFIDEHCE INTERVAL IS . 156 176 COHO SMOLT (258 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE OU 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 10 10 10 10 10 10 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.1000 0.1000 0-2000 0.9000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 166 THE 95% CONFIDENCE INTERVAL IS 156 176 1
COHO FRY (18 HOURS)
MAY 5, 1982 PROJECT 4Ar DILUTER D 103 TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 VHE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1786 0.7667 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 79 THE 95% CONFIDENCE INTERVAL IS 75 83 COHO FRY (25 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1786 0.8667 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 78 THE 95% CONFIDENCE INTERVAL IS 74 81 COHO FRY (41 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 HIE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1786 0.9333 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 77 THE 95% CONFIDENCE INTERVAL IS 73 81 COHO FRY (90 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPURTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1786 0.9333 1.0000 THE SPEARMAN KARBER LC50 ESTIMA1E IS 77 THE 95% CONFIDENCE INTERVAL IS 73 81
I COHO FRY (138 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAH-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0333 0.0000 0.0333 0.2143 0 9333 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 76 THE 95% CONFIDENCE INTERVAL IS 72 80 C0HO FRY (162 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 20 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0333 0.0000 0.0333 0.3714 0.9333 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 73 THE 95% CONFIDENCE INTERVAL IS 69 78 COHO FRY (185 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 88 104 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0333 0.0000 0.0333 0.3929 0.9333 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 72 THE 95% CONFIDENCE INTERVAL IS 68 76 COHO FRY (258 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 02 43 53 70 38 104 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0667 0.0000 0.0333 0.3929 0.9333 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 72 THE 95% CONFIDENCE INTERVAL IS 68 76
COHO FRY (18 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1786 0.7667 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 172 THE 95% CONFIDENCE INTERVAL IS 164 179 COHO FRY (25 HOURS)
MAY 5e 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE. 08 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1786 0.8667 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 168 THE 95% CONFIDENCE INTERVAL IS 162 174 COHO FRY (41 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.1786 0.9333 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 166 THE 95% CONFIDENCE INTERVAL IS 160 173 COHO FRY (90 HOURS)
MAY 5, 1982 PROJECT 6As DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.1786 0.9333 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 166 VHE 95% CONFIDENCE INTERVAL IS 161 172
COHO FRY (138 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0333 0.0000 0.0333 0.2143 0.9333 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 165 THE 95% CONFIDENCE INTERVAL IS 159 171 C0HO FRY (162 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0333 0.0000 0.0333 0.3214 0.9333 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 162 THE 95% CONFIDENCE INTERVAL IS 155 168 COHO FRY (185 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPORTION DEAD IN 96 HOURS WERE 0.0333 0.0000 0.0333 0.3929 0.9333 1.0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 159 THE 95% CONFIDENCE INTERVAL IS 153 166 COHO FRY (258 HOURS)
MAY 5, 1982 PROJECT 6A, DILUTER D 10% TRIMMED SPEARMAH-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 08 109 132 154 186 233 THE NUMBER QF FISH IN RESPECTIVE TANK 30 30 30 28 30 29 THE PROPCRTION DEAD IN 96 HOURS WERE 0,0667 0.0000 0.0333 0.3929 0.9333 1 0000 0.0 THE SPEARMAN KARBER LC50 ESTIMATE IS 159 THE 95% CONFIDENCE INTERVAL IS 153 166
O PROJECT 4 SUBACUTE
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COHO FRY (819 HOURS)
COPPER MAY 28, 1982 PROJECT 6S DILUTERS B & C 10% TRIMMED SPEARMAN-KARBER ANALYSIS -
THE DOSES (PARTS PER BILLION) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60 60 60 THE PROPORTION DEAD IN 96 HOURS WERE , 0.0000 0.0500 0.1000 0.1833 0.6833 0.9107 THE SPEARMAN KARBER LC50 ESTIMATE IS 45 THE 95% CONFIDENCE INTERVAL IS 43 48 C0HO FRY (843 HOURS)
COPPER MAY 28, 1982 PROJECT 6S DILUTERS B $ C 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60 60 60 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1167 0.1833 0.7333 0.9107 THE SPEARMAN KARBER LC50 ESTIMATE IS 44 THE 95% CONFIDENCE INTERVAL IS 42 47 COHO FRY (867 HOURS)
COPPER MAY 28, 1982 PROJECT 6S DILUTERS B 1 C 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60 60 60 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1167 0.2000 0.7833 0.9464 THE SPEARMAN KARBER LC50 ESTIMATE IS 43 i
THE 95% CONFIDENCE INTERVAL IS 41 45 f
f COHO FRY (891 HOURS) i COPPER MAY 28, 1982 PROJECT 6S DILUTERS B 1 C 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLIGH) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60 60 60 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1167 0.2167 0.8000 0.9464 THE SPEARMAN KARBER LC50 ESTIMATE IS 42 THE 95% CONFIDENCE INTERVAL IS 40 45 L
COHO FRY (915 HOURS)
COPPER MAY 28, 1982 PROJECT 6S DILUTERS B 1 C 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60 60 60 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1167 0.2167 0.8000 0.9643 THE SPEARMAN KARBER LC50 ESTIMATE IS - 42 THE 95% CONFIDENCE INTERVAL IS 40 45 COHO FRY (940 HOURS)
COPPER MAY 28, 1982 PROJECT 6S DILUTERS B 1 C 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60 60 60 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1167 0.2167 0.8167 0.9643 THE SPEARMAN KARBER LC50 ESTIMATE IS 42 THE 95% CONFIDENCE INTERVAL IS 40 44 COHO FRY (964 HOURS)
COPPER MAY 28, 1?S2 PROJECT 69 )!LUTERS B 1 C 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60 60 60 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1167 0.2167 0.8333 0.9821 THE SPEARMAN KARBER LC50 ESTIMATE IS 42 THE 951 CONFIDENCE INTERVAL IS 40 44 COHO FRY (987 HOURS)
COPPER MAY 28, 1982 PROJECT 6S DILUTERS B 1 C 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60 60 60 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1167 0.2333 0.8333 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 42 THE 95% CONFIDENCE INTERVAL IS 40 44
COHO FRY (1010 HOURS)
COPPER MAY 28, 1982 PROJECT 6S DILUTERS B 1 C 10% TRIMMED SPEARMAN-KARBER ANALYSIS TPE DOSES (PARTS PER BILLION) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60 60 60 THE PROP 09 TION DEAD IN 96 HOURS WERE 0.0000 0.0500 0.1167 0.2333 0.8667 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 41 THE 95% CONFIDENCE INTERVAL IS 39 43 C0HO FRY (1035 HOURS)
COPPER MAY 26, 1982 PROJECT 6S DILUTERS B 1 C 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60 60 60 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0667 0.1167 0.2500 0.9000 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 41 THE 95% CONFIDENCE INTERVAL IS 39 42 COHO FRY (1059 HOURS)
COPPER MAY 28, 1982 PROJECT 6S DILUTERS B & C 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DDSES (PARTS PER BILLION) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60' 60 60 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0667 0.1167 0.2500 0.9176 1.0000 THE SPEARMAN KARBER LC50 ESTIMATE IS 40 THE 95% CONFIDENCE INTERVAL IS 39 42 i
I COHO FRY (1440 HOURS)
COPPER MAY 28, 1982 PROJECT 6S DILUTERS B & C 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLIGH) WERE 19 21 33 34 53 65 THE NUMBER OF FISH IN RESPECTIVE TANK 60 60 60 60 60 60
, THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0667 0.1500 0.3000 1.0000 1.0000 l
l THE SPEARMAN KARBER LC50 ESTIMATE IS 39 THE 95% CONFIDENCE INTERVAL IS 37 40 L - - _ _ _ _
PROJECT 7 w...
l l
COHO FRY (76 HOURS)
COPPER SEPTEMBER 16,1982 DILUTERS A 1 C 101 TRIMEB SPEARMAN-KARBER ANALYSIS 44 48 60 75 81 96 121 142 TE DOSES (PARTS PER BILLION) ERE 18 29 30 30 30 30 30 30 30 30 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 THE PROPORTION KAD IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0000 0 00M 0 0000 0 0000 01333 0.96671.M00 TE SPEARMAN KARER LC50 ESTIMTE IS 106 102 110 TE 951 CONFIDENCE INTERVAL IS C0HO FRY (78 HOURS)
COPKR SEPTEMER 16,1982 DILUTERS A 1 C 103 TRIMMED SPEARMAN-KARBER ANALYSIS 29 46 48 60 75 81 96 121 142 TE DOSES (PARTS PER BILLION) WERE 18 30 30 30 30 30 30 30 30 30 THE MUMER OF FISH IN RESPECTIVE TANK 30 THE PROPORTION K AD IN 96 HOURS ERE 0.0000 0.0000 0.0M0 0.0000 0.00M 0.0333 0.0M0 0.20001.00001.0000 TE SPEARMN KARBER LC50 ESTIMTE IS 104 THE 951 CONFI ENCE IM ERVAL IS 100 108 C0HO FRY (100 HOURS)
COPPER SEPTEMBER 16,1992 DILUTERS A 1 C 101 TRIMMED SPEARMAN-KARER ANALYSIS 29 46 48 60 75 81 96 121 142 THE DOSES (PARTS PER BILLION) WERE 18 30 30 30 30 30 30 30 30 TE NUMER OF FISH IN RESPECTIVE TANK 30 30 TE PROPORTION KAD IN 96 HOURS ERE 0.00M 0.0M0 0.0000 0.0M0 0.0333 0.0333 0.0000 0.23331.000010000 THE SPEARMAN KARER LC50 EST!MTE IS 103 THE 951 CONFIDENCE INTERVAL IS 99 100 C0HO FRY (118 HOURS)
' COPPER SEPTEMBER 16,1982 DILUTERS A 1 C 101 TRIMED SPEARMAN-KARER ANALYSIS 29 46 48 60 75 81 96 121 142 THE DOSES (PARTS PER BILLION) ERE 18 30 30 30 30 30 30 30 30 TE NUMBER OF FISH IN RESPECTIVE TALE 30 30 TE PROPORTION KAD IN 96 HOURS ERE 0.0000 0.00M 0.00M 0.00M 0.0333 0.0333 0.0004 0.26671.00001.0000 TE SPEARMAN KARER LC50 ESTIMTE IS 103 THE 951 COWI ENCE INTERVAL IS 98 107
C0HO FRY (124 HOURS)
COPPER SEPTEMKR 16,1982 DILUTERS A 1 C 10% TRIMED SPEARMAN-KARBER AMLYSIS THE ImSES (PARTS PER BILLION) ERE 18 29 46 48 60 75 81 96 121' 142 THE NUME R OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE F1tbPORTION KAB IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0000 0.0333 0.0333 0.$333 0.3000 1.0000 1.0000 TE SPEARMAN KARER LC50 ESTIMTE IS 102 THE 95I CONFIDOCE INTErtVAL IS 98 106 COHO FRY (143 HOURS)
CCPPER SEPTEMBER 16,1982 DILUTERS A 1 C 10% TRIMMED SPEARMA H AR KR AMLYSIS TE DOSES (PARTS PER BILLION) ERE 18 29 46 48 60 75 81 96 121 142 THE NUMER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 TE PROPORTION KAD IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0000 0.0333 0.1000 0.1333 0.3667 1.0000 1.0000 THE SPEARMAN KARBER LC50 ESTIMTE IS 99 THE 95% CONFI ENCE INTERVAL IS 94 104 C0HO FRY (150 HOURS)
COPPER -
SEPTEMER 16,1982 LILUTERS A 1 C 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) ERE 18 29 46 48 60 75 81 96 121 142 TE NUMBER OF FISH IN RESPECTIVE TAM 30 30 30 30 30 30 30 30 30 30 THE PROPORTION K AD IN 96 HOURS E RE 0.0000 0.0000 0.0000 0.0000 0.0333 0.1333 0.1667 0.4333 1.0000 1.0000 THE SPEARMAN KARER LC50 ESTIMTE IS 97 THE 951 CONFIDO CE INTERVAL IS 92 102 C0HO FRY (167 HOURS)
COPPER SEPTEMER 16,1982 DILUTERS A 1 C 10% TRIMMED SPENtMN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) ERE 18 29 46 48 60 75 81 96 121 142 THE NUME R OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 TE PROPORTION KAB IN 96 HOURS WERE 0.0000 0.0000 0.0333 0.0000 0.0333 0.1000 0.3667 0.6667 1.0000 1.0000 TE SPEARMN KARER LC50 ESTIMTE IS 89 THE 951 CONFIK NCE INTERVAL IS 85 93
COHO FRY (191 H0lRS)
COPPER SEPTEMBER 16,1982 DILUTERS A 1 C SGI TRINED SPEARMAN-KARER ANALYSIS TE DOSES (PARTS PER BILLION) ERE 18 29 46 48 60 75 81 96 121 142 TE NUMER OF FISH IN RESPECTM TANK 30 30 30 30 30 30 30 30 30 30 TE PROPORTION BEAR IN 96 HOURS ERE 0.0000 0.0000 0.0667 0.0000 0.2000 0.3000 0.6000 0.8000 1.0000 1.0000 THE SPEAMAN KARBER LC50 ESTIMTE IS 78 THE 95I CONFIDENCE INTERVAL IS 73 83 COHO FRY (215 HOURS)
COPPEL SEFTEMBER 16e 1982 DILUTER $ A 1 C 103 TRIMED SPEARMN-KARBER ANALYSIS TE DOSES (PARTS PER BILLION) ERE 18 29 46 48 60 75 81 96 121 142 THE MlplBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PP0 PORTION KAB IN 96 HOURS ERE 0.0000 0.0000 0.1333 0.0000 0.3333 0.5333 0.0000 0.9000 1 0000 1.0000 TE SPEARNAM KARBER LC50 ESTIMTE IS 69 THE 951 CONFI ENCE INTEkVAL IS 64 73 i
l __ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
00HO FRY (46 HOURS)
SEPTEMBER 16,1982 DILUTERS B 1 D ,
10% TRIMMED SPEARMN-KARER AMLYSIS THE DOSES (PARTS PER BILLION) ERE 18 30 47 49 57 73 78 86 113 134 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION KAD IN 96 H0(RS ERE 0.0000 0.0000 0.0000 0.0000 0 0000 0.0000 0.1000 0.4467 0.9333 1.0000 THE SP5NWAN KARBER LC50 ESTIMTE IS 90 THE 95% CONFIDENCE INTERVAL IS 86 94 COHO FRY (53 HOURS)
SEPTEMER 16,1982 DILUTERS B 1 D 10% TRIMED SPEARMAN-KARKR AMLYSIS THE DO3ES (PARTS PER BILLION) WERE 18 30 47 49 57 73 78 86 113 134 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 TE PROPORTION DEAD IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.1333 0.4667 0.9667 1.0000 THE SPEARMN KARKR LC50 ESTIMTE IS 89 TE 95% CONFIDENCE INTERVAL IS 84 93 COHO FRY (70 HOURS)
SEPTEMKR 16,1982 DILUTERS B 1 D 10% TRIMMED SPEARMAN-KARBER AHLYSIS THE D0cES (PARTS PER BILLION) ERE 18 30 47 49 57 73 78 86 113 134 THE MlmBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION KAD IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0M0 0.M00 0.1333 0.2000 0.63331.00001.0000 TE SPEARMAN KARBER LC50 ESTIMTE IS 85 THE 951 CONFIDENCE INTERVAL IS 81 89 C0HO FRY (78 HOURS)
SEPTEMBER 16,1982 DILUTEES B 1 D 10: TRIMMED STEARMAN-KAR E R AH LYSIS THE DOSES (PARTS PER BILLION) WERE 18 30 47 49 57 73 78 86 113 134 THE NUMER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 TE PP0 PORTION MAD IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0M0 0.0000 0.1333 0.2000 0 66671.00001.M00 1E SPEARMAN KARBER LC50 ESTIMTE IS 84 THE 951 C0K IDENCE INTERVAL IS 81 88
COMO FRY (94 HOURS)
COPPEE/ZIE (COPPER)
SEPTENKR 16,1992 DILUTE *3 9 1 D
=. 101 TRIMED SPEARMit-KARKR AMLYSIS TE DOSES (PARTS PLR IILLION) ERE 18 30 47 49 57 73 78 86 113 134 TE NUMBER OF FISH IN ESPECTIVE Tale ( 30 30 30 30 30 30 30 30 30 30 THE PPOPORTION DEAD IN 96 HOURS E RE 0.0000 0.0000 0.0000 0.0000 0.0000 0.1667 0.2333 0.6667 1.0000 1.0000 TE SPEARMM KARER LC50 ESTIMTE IS 84 TE 952 CONFlKEE INTERVAL IS 80 88 COHO FRY (100 HOURS)
SEPTEMK5.16e 1982 DILUTERS B 1 D 101 TRIMMED SPEARMAlH(ARER AMLYSIS THE DOSES (PARTS PER BILLION) E RE 18 30 47 49 57 73 78 86 113 134 THE NUM0ER OF FISH IN KSPECTIVE TAIAC 30 30 30 30 30 30 30 30 30 30 TE PROP 0RT10ll KAB IN 96 HOWS ERE 0.0000 0.0000 0.0000 0.0000 0.0000 0.2333 0.2333 0.4467 1.0000 1.0000 TE SPEARMM KARER LC50 ESTIMTE IS 83 TE 95I CONFIENCE INTERVAL IS 79 87 C0HO FRY (124 HOURS)
SEPTEMER 16,1982 DILUTERS B I D 103 TRIMED SPEARMM-KARKR AMLYSIS THE DOSES (PARTS PER BILLION) KRE 18 30 47 49 57 73 78 84 113 134 THE hUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROP 0RTION MAD IN 96 HOW S nlEE 0.0000 0.0000 0.0000 0.0000 0.0000 0.2333 0.3333 0.6667 1.0000 1.0000 THE SPEARMM KARER LC50 ESTIMTE IS 82 THE 951 CONFlKEE INTERVAL IS 78 84 COHG FRY (143 HOW S)
COPPER /ZI K (COPftR)
SEPTEMER 16,1982 DILUTERS B 1 D 10% TRIMMED SPEARMAlt-KARKR AllALYSIS THE DOSES (PARTS PER BILLION) ERE 18 30 47 49 57 73 78 86 113 134 THE NUMBER OF FISH IN ESPECTIVE Tale ( 30 30 30 30 30 30 30 30 30 30 THi. PROPORTION K AB IN 96 HOWS KRE 0.0000 0.0000 0.0000 0.0000 0.0000 0.3000 0.3333 0.6667 1.0000 1.0000 THE SPEARMM KARSER LC50 ESTIMTE IS 81 TE 951 CONFIKEE INTERVAL IS 77 85
l l
COHO FRY (150 H0lRS)
SEPTEMER 16,1982 DILUTERS B & D 10% TRIMMEB SPEARMAN-KARER AMLYSIS TE 00SES (PARTS PER BILLION) E 18 30 47 49 57 73 78 86 113 134 TE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 TE PROPORTION KAB IN 96 HOURS KRE 0.0000 0.0000 0.0000 0.0000 0.0000 0.3000 0.3333 0.7000 1.0000 1.0000 TE SPEARMAN KARER LC50 ESTIMTE IS 80 TE 95% CONFIDENCE INTERVAL IS 77 85 COHO FRY (167 HOURS)
SEPTEMBER 16,1982 DILUTERS B & D 10% TRIMMED SPEARMAN-KARBER AMLYSIS THE DOSES (PARTS PER BILLION) WERE 18 30 47 49 57 73 78 86 113 134
!E NUMER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION KAD IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0000 0.0333 0.3333 0.4000 0.7333 1.0000 1.0000 THE SPEARMAN KARER LC50 ESTIMTE IS 79 TE 95% CONFIDEEE INTERVAL IS 75 83 C0HO FRY (191 HOURS)
SEPTEMER 16,1982 DILUTERS B t D 10% TRIMMED SPEARMAN-KAR ER ANALYSIS THE DOSES (PARTS PER BILLION) ERE 18 30 47 49 57 73 78 86 113 134 THE NUMER OF FISH IN RESPECTIVE TAMK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION KAB IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0000 0.1000 0.4000 0.5667 0.7667 1.0000 1.0000 TE SPEARMAN KARER LC50 ESTIMTE IS 75 THE 95% CONFIDENCE INTERVAL IS 71 79 C0HO FRY (215 HOURS)
COPPER / ZINC (COPPERS SEPTEMER 16,1982 DILUTERS B 1 D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) ERE 18 30 47 49 57 73 78 86 113 134 TE NUMBER OF FISH IN RESPECTIVE TAMK 30 30 30 30 30 30 30 30 30 30 THE PROP 0RTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0333 0.1667 0.4333 0.7000 0.8333 1.0000 1.0000 TE SPEARMN KARER LC50 ESTIMTE IS 72 l
THE 95% CONFIDENCE INTERVAL IS 68 76
~
COHO FRY (46 HORS)
SEPTEMER 16,1982 DILUTER 3 8 1 D 1031 RIMMED SPEIJtMAN-KARBER AMLYSIS TE DOSES (PARIS PER BILLION) ERE 81 108 148 1M 174 215 266 273 349 385 TE NUMBER OF FISM IN RESPECTIVE TN 30 30 30 30 30 30 30 30 30 30 TE PROPORTION MAD IN 96 HORS ERE 0.00M 0.0000 0.0000 0.0000 0.0000 0.0M0 0.1000 0.447 0.93331.00M TE SPEARMAN KARBER LC50 ESTIMTE IS 289 TE 951 COEIBOKE INTERVAL IS 279 298 C0HO FRY (53 HOIRS)
SEPTEMER 16,1982 DILUTERS B 1 D 103 TRIMMED SPEARMAN-KARBER AMLYSIS THE DOSES (PARTS PER BILLION) ERE 81 108 148 1M 174 215 266 273 349 385 THE NUM E R OF FISH IN RESPECTIVE T N 30 30 30 30 30 30 30 30 30 30 THE PROPORTION M AD IN 96 HOURS E RE 0.0000 0.0000 0.0M0 0.0000 0.0000 0.00 M 0.1333 0.4467 0.9667 1.0000 THE SPEARMAN KARBER LC50 ESTIMTE IS 287 THE 95% COEIKKE INTERVAL IS 278 297 C0HO FRY (70 HOURS)
C0f MR/IINC (ZINC)
SEPTEMER 16,1932 DILUTERS B 1 D 103 TRIMMED SPEARMAN-KAR K R A MLYSIS THE DOSES (PARTS PER BILLION) ERE 81 108 148 1M 174 215 266 273 349 385 THE NUMBER OF FISP IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION K AD IN 96 HOURS ERE 0.00M 0.0000 0.0M6 0.0000 0.0000 0.1333 0.20M 0.633310M01.0000 TE SPEARMAN KARE LC50 ESTIMTE IS 273 TE 95% CONFIENCT. INTERVAL IS 262 285 C0HO FRY (78 HOURS) 00PKR/ ZINC (ZINC)
SEPTEMER 16,1982 DILUTERS B 1 D 102 TRIMMED SPEARMAN-KAR E R ANALYSIS THE DOSES (PARTS PER BILLION) E RE 81 108 148 1H 174 215 266 273 349 385 L
THE NUMBER OF FISH IN RESPECTIVE Tale ( 30 30 30 30 30 30 30 30 30 30 THE PROPORTION K AD IN 96 HOURS WERE 0.0000 0.00M 60000 0.0000 0.0000 0.1333 0.20M 0.66671.00001.0M0 TE SPEARMAN KARBER LC50 ESTIMTE IS' 272 T E 951 CONFI ENCE INTERVAL IS 261 283 l
l L
C0HO FRY (94 HOURS)
SEPTEMKR 16,1982 DILUTERS B 1 D 10% TRIMMED SPEARMHARKR AMLYSIS THE DOSES (PARTS PER BILLION) M 01 100 148 140 174 215 266 273 349 385 TE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROP 0RTION KAB IN 96 HOURS E RE 0.M 0.0000 0.M 0.M 0.M 0.1667 0.2333 0.66671.00001.M THE SPEARMAN KAR ER LC50 ESTI M TE IS 269 T E 951 CONFIDENCE INTERVAL IS 256 281 C0HO FRY (100 HOURS)
SEPTEMER 16,1982 DILUTERS B & D 10% TRIMMED SPEARMA H ARBER ANALYSIS THE DOSES (PARTS PER BILLION) WERE 81 108 148 140 174 215 266 273 349 385 TE NUMBER OF FISH IN RESPECTIE TALE 30 30 30 30 30 30 30 30 30 30 TE PROPORTION KAB IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0000 0.0000 0.2333 0.2333 0.66671.00001.M THE SPEARMAN KARBER LC50 ESTIMTE IS 264 THE 951 CO W I ENCE INTERVAL IS 251 278 CCHO FRY (124 HOURS)
SEPTEMER 16,1982 DILUTERS B 1 0 101 TRIMED SPEARMAN-KARER AHLYSIS THE DOSES (PARTS PER BILLION) WERE 81 108 148 140 174 215 266 273 349 385 TE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 TE PROFORTION KAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.0000 0.2333 0.3333 0.6667 1.0000 1.0000 TE SPEARMAN KARKR LC50 ESTIMTE IS 261 THE 951 CONFIDENCE INTERVAL IS 247 275 C0HO FRT (143 HOURS)
SEPTEMER 16,1982 D!LUTERS B 1 D 10% TRIMED SPEARMAN-KARBER ANALYSIS fHE DOSES (PARTS PER BILLION) ERE 81 108 148 140 174 215 266 273 349 385 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 1E PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0000 0.0000 0.3000 0.3333 0.6667 1.0000 1.0000 TE SPEARMAN KARBER LC50 ESTIMTE IS 256 THE 951 CONFIDENCE INTERVAL IS 242 271
CCHO FRT (150 HOURS)
CDPFER/ ZINC (ZIE)
SEPVEMMR 16,1932 DILUTERS B 1 D 10: TRIMED SPEARMAN-KARER AMLYSIS TE DOSES (PARTS PER BILLION) ERE 81 108 148 le 174 215 266 273 349 385 VE NUMER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 VE PROFORTION KAD IM 96 HOURS KRE 0.0000 0.M 0.0000 0.0000 0.0000 0.3000 0.3333 0.70001.C0001.0000 THE SPEARMAN KMER LC50 ESTIMTE IS 255 THE 951 CONFI KMCE INTERVAL IS 241 269 C0HO FRT (167 HOURS)
SEPTEMBER 16,1982 DILUTERS B 1 D 103 TRlhMED SPEARMAN-KAR ER AM LYSIS THE DOSES (PARTS PER BILLION) K RE 81 108 148 140 174 215 266 273 349 385 TE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION KAD IN 96 HOURS KRE 0.0000 0.0000 0.0000 0.M 0.0333 0.3333 0.4000 0.73331.0A01.0000 I
TE SPEARMAN KARER LC50 ESTIMTE IS 248 THE 95% COEIKKE INTERVAL IS 235 263 C0HO FRT (191 HOURS)
SEPTEMER 16,1982 DILUTERS B 1 D 10% TRIhMED SPEAR M N-KARER ANALYSIS THE DOSES (PARTS PER BILLION) ERE 81 108 148 140 174 215 266 273 349 385 THE NUMKR OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROP 0RTION K AD IN 96 HOURS KRE 0.0000 0.0000 0.0000 0.0000 0.1000 0.4000 0.5667 0.7667 1.0000 1.0000 THE SPEARMN KARER LC50 ESTIMATE IS 235 TE 95I CONFIDENCE INTERVAL IS 222 250 C0HG FRY (215 HOURS)
SEPTEMBER 16,1982 DILUTERS B 1 D 103 TRIMMED SPEARMAN-KAR ER ANALYSIS T E DOSES (PARTS PER BILLION) ERE 81 108 148 140 174 215 266 273 349 385 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION K AD IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0333 0.1667 0.4333 0.7000 0.8333 1.0000 1.0 M THE SPEARMN KARER LC50 ESTIMTE IS 224 V E 951 CCNFIENCE INTERVAL IS 210 239
STEELEAD FRY (53 HOURS)
COPPER SEPTEMBER 16,1982 DILUTERS A 8 C 10% TRIND SPORMN-KAR9ER AMLYSIS -
TE DOSES (PARTS PER BILLION) ERE 18 29 46 48 60 75 81 96 121 142 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 29 30 THE PROPORTION DEAD IN 96 H0lRS ERE 0.0000 0.0000 0.0000 0.0000 0.0000 0. W 7 0.1000 0.2667 0.7419 0.9333 THE SPEARN KARER LC50 ESTIMTE IS 107 TE 951 CONFIDEHCE INTERVAL IS 101 114 STEELHEAD FRY (70 MOURS)
COPPER SEPTEMKR 16,1982 DILUTERS A 8 C 10% TRIMMED SPEARMAN-KARSER AMLYSIS THE DOSES (PARTS PER BILLION) WERE 18 29 46 48 60 75 81 96 121 142 TE NUMER OF FISH IN RESPECTIVE TAE 30 30 30 30 30 30 30 30 29 30 THE PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0000 0.0000 0.0333 0.0000 0.1667 0.1333 0.3333 0. W 7 1.0000 THE SPORN KARIER LC50 ESTIMTE IS 100 l THE 95% CONFIDENCE INTERVAL IS 95 105 STEELEAD FRY (78 HOURS)
COPPER SEPTEMER 16,1982 DILUTERS A 1 C 10% TRIMMED SPEARMAN-KARBER AHLYSIS THE DOSES (PARTS PER BILLION) ERE 18 29 46 48 60 75 81 96 121 142 l THE NUMER OF FISH IN RES. N CTIVE TAE 30 30 30 30 30 30 30 30 29 30 THE PROPORTION DEAB IN 96 HOURS PERE 0.0000 0.0000 0.0333 0.1667 0.2000 0.2000 0.3000 0.5667 0.9355 1.0000 c THE SPEARN KARER LC50 ESTIMTE IS 87 l
THE 95% CONFI KNCE INTERVAL IS 81 94 I
l l STEELEAD FRY (100 H0lRS)
C0PPER SEPTEMIER 16,1982 DILUTERS A 8 C 10% TRIMMED SPEARMN-KAKXR ANALYSIS TE DOSES (PARTS PER BILLION) WERE 18 29 46 48 60 75 81 96 121 142 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 29 30 TE PROPORTION KAB IN 96 HOIRS ERE 0.0000 0.0000 0.1000 0.1647 0.2000 0.3333 0 3667 0.6333 0.9355 1.0000 TE SPORMAN KARSER LC50 ESTIMTE IS 83 THE 95% CONFIDENCE INTERVAL IS 77 90
SVEELHEAD FRY (118 HOURS)
COPMR SEPTEMBER 16,1982 DILUTERS A 1 C 102 TRIMMED SPEARMN-KARER AMLYSIS M DOSES (PARTS PER BILLION) E RE 18 29 46 48 60 75 81 96 121 142 TE NUMER OF FISH IN RESPECTIVE TAE 30 30 30 30 30 30 30 30 29 30 TE PROPORTION MA8 IN 96 HOURS WERE 0.00000.03330.00000.26670.26670.36670.50000.73331.Q0001.0000 M SPEARMAN KARER LC50 ESTIMTE IS 76 VHE 95% CONFI KNCE INTERVAL IS 70 82 STEELE AR FRY (124 HOURS)
COPPER SEPTEMKR 16,1982 DILUTERS A 1 C 10% TRIMMED SPEARMAN-KAR E R ANALYSIS THE DOSES (PARTS PER IILLION) ERE 18 29 46 48 60 75 81 96 121 142 TE NUMKR OF FISH IN RESPECTIVE TAE 30 30 30 30 30 30 30 30 29 30 VHE PROPORTION KAB IN 96 HOURS ERE 0.0000 0.1000 0.1333 0.3333 0.3000 0.4667 0.6333 0.7333 1.0000 1.0000 TE SPEARMAN KARER LC50 ESTIMTE IS 70 THE 951 COEIKNCE INTERVAL IS 64 77 STEELEAR FRY (143 HOURS)
COPPER SEPTEMBER 16,1982 DILUTERS A 1 C IOK TRIMED SPEARMAN-KARKit ANALYSIS TE POSES (PARTS PER BILLION) ERE 18 29 46 48 60 75 81 96 121 142 TE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 29 30 TE PROPORTION KAD IN 96 H0(RS ERE 0.0000 0.1000 0.2333 0.4000 0.3333 0.5000 0.6667 0.7667 1.0000 1.0000 TE SPEARMAN KARBER LC50 ESTIMTE IS 65 TE 951 CONFIDENCE INTERVAL IS 57 72 STEELHEAD FRY (150 HOURS)
COPPCR SEPTEMKR 14,1982 l
DILUTERS A 1 C 103 TRIMED SPEARMM-KARKR ANALYSIS TE DOSES (PARTS PER BILLION) ERE 18 29 46 48 60 75 81 96 121 142 TE NUPBER OF FISH IN RESPECTIVE TAE 30 30 30 30 30 30 30 30 29 30 TE PROPORTION MAD IN 96 H0(RS WERE 0.0000 0.1000 0.3000 0.4000 0.3333 0.5667 0.6667 0.7667 1.0000 1.0000 TE SPEARMU KARER LC50 ESTIMTE IS 63 TE 951 CONFIDENCE INTERVAL IS 57 70
STE lHEAD FRT (167 HOURS)
C0FFER SEFTEMKR 16,1982 DILUTERS A 1 C 101 TRIMMED SPEARMA H ARBER ANALYSIS TE DOSES (PARTS PER BILLION) ERC 18 29 46 48 60 75 81 96 121 142 THE NLMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 29 30 THE PROPORTION KAD IN 96 HOURS ERE 0.0000 0.1000 0.3000 0.4000 0.3667 0.6000 0.7333 0.8000 1.0000 1.0000 TE SPEARMAN KAR ER LC50 ESTIMTE IS 61 THE 951 CONFIDDICE INTERVAL IS 56 68 STEELHEAD FRY (191 HOLRS)
COPPER SEPTEMBER 16,1982 DILUTERS A 1 C 10% TRIMMED SPEARMA H AR E R ANALYSIS TK DOSES (PARTS PER BILLION) E RE 18 29 46 48 60 75 81 96 121 142 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 29 30 TE PkOPORTION KAD IN 96 HOURS ERE 0.0000 0.1000 0.3000 0.4000 0.4333 0.7000 0.8000 0.8333 1.0000 1.0000 THE SPEARMAN KARER LC50 ESTIMTE IS 58 T E 95% CONFIE NCE INTERVAL IS 53 64 STEELHEAD FRf (215 HOURS)
COPPER SEPTEMER 16,1982 DILUTERS A 1 C 101 TRIMMED SPEARMAN-KARER ANALYSIS TE DOSES (PARTS PER BILLION) ERE 18 29 46 48 60 75 81 96 121 142 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30' 29 30 THE PROPORTION KAD IN Y6 HOURS E RE 0.0000 0.1000 0.3000 0.4000 0.4667 0.8333 0.8333 0.9000 1.0000 1.0000 TE SPEARMN KARBER LC50 ESilMTE IS 55 TE 951 CONFIENCE INTEFVAL IS 50 61
STEELHEAD FRY (46 HOURS)
SEPTEMER 16,1982 DILUTERS B 1 D 10% TRIMMED SPEARMN-KARER ANALYSIS T)'I DOSES (PARTS PER IILLION) ERE 18 30 47 49 57 73 78 86 113 134 TE NUMER OF FISH IN ESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 TE PROPORTION DEAD IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0000 0.0333 0.1667 0.4000 0.6333 0.9333 0.9667 TE SPEARMAN KARER LC50 ESTIMTE IS 83 THE 951 CONFI E NCE INTERVAL IS 79 88 STEELHEAD FRY (53 HOURS)
SEPTEMER 16,1982 DILUTERS B 1 D 101 TRIMMED SPEARMAN-KARKR AMLYSIS TE DOSES (PARTS PER BILLION) ERE 18 30 47 49 57 73 78 86 113 134 TE NUMER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 TE PROPORTION KAB IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0000 0.0333 0.2647 0.6000 0.8000 0.9333 1.0000 TE SPEARMAN KARER LC50 ESTIMTE IS 77 TE 951 CONFIKNCE INTERVAL IS 74 81 STEELHEAB FRY (70 HOURS)
SEPTEMBER 16s 1982 DILUTERS B & D 10% TRIMMED SPEARMM-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) ERE 18 30 47 49 57 73 78 05 113 134 THE NUMER OF FISH IN RESPECTIVE TAE 30 30 30 30 30 30 30 30 30 30 THE PROPOLTION K AD IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0000 0.0333 0.4000 0.8000 0.9000 1.0000 1.0000 TE SPEARMAN KARKR LC50 ESTIMTE IS 72 TE 95% CONFIENCE INTERVAL IS 70 75 STEELEAS FRY (78 HOURS)
SEPTEMKR 16,1982 DILUTERS B 1 D 10% TRIMMED SPEARMAN-KldKR AMLYSIS THE DOSES (PARTS PER BILLION) ERE 18 30 47 49 57 73 78 84 113 134 THE NUM E R OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 TE PROPORTION KAD IN 96 HOWS ERE 0.0000 0.0000 0.0000 0.0333 0.1000 0.4333 0.8000 0.9667 1.0000 1.0000 TE SPEARMAN KARBER LC50 ESTIMTE IS 71 THE 951 CO EIDENCE INTERVAL IS 60 74
STEELEAD FRY (94 HOURS)
SEPTEMER 16,1992 DILUTERS B 1 D 10% TRIMMED SPEARMHARBER ANALTSIS TE BOSES (PART3 PER BILLION) ERE 18 30 47 49 57 73 78 86 113 134 THE NUM KR OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 TE PROPORTION KAD IN 96 HORS ERE 0.0000 0.0000 0.0000 0.0333 0.1667 0.5000 0.8000 0.9667 1.0000 1.0000 THE SPEARMAN KARER LC50 ESTIMTE IS 69 TE 95% CONFIKNCE INTERVAL IS 66 73 STEELEAD FRY (100 HORS)
SEPTEMER 16,1982 DILUTERS B 1 D 10% TRIMED SPEARMAHARER ANALYSIS THE DOSES (PARTS PER BILLION) H 18 30 47 49 57 73 78 84 113 134 TE NUMBER OF FISH IN KSPECTIE TN 30 30 30 30 30 30 30 30 30 30 TE PROPORTION KAB IN 96 HOURS ERE 0.0000 0.0333 0.0333 0.0333 0.1667 0.5000 0.8467 0.9667 1.0000 1.0000 THE SPEARMAN KARER LC50 ESTIMTE IS 69 TE 95% CONFIKNCE INTERVAL IS 66 72 STEELEAD FRY (124 HOURS)
COPMR/ ZINC (C0FPER)
EEPTEMER 16,1982 DILUTERS B 1 D 10% TRIMMED SPEARMAN-KARBER ANALYSIS THE DOSES (PARTS PER BILLION) ERE 18 30 47 49 57 73 78 86 113 134 THE NUM ER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION K AB IN 96 HOURS ERE 0.0000 0.0333 0.0333 0.0333 0.2000 0.5333 0.8667 0.9667 1.0000 1.0M0 THE SPEARMAN KARBER LC50 ESTIMTE IS 68 TE 95% CONFIKWCE INTERVAL IS 65 72 STEEL EAD FRY (167 HOURS)
SEPTEMER 16,1982 DILUTERS B 1 D 10% TRIMMED SPEARMAN-KARBER ANALYSIS TE DOSES (PARTS MR BILLION) ERE 18 30 47 49 57 73 78 86 113 134 TE NUMER OF FISH IN RESPECTIVE TAE 30 30 30 30 30 30 30 30 30 30 TE PROPORTION KAD IN 96 HOWS ERE 0.0000 0.0333 0.0333 0.0333 0.2000 0.5667 0.8647 0.9667 1.0000 1.0000 TE SPEARMAN KAR9ER LC50 ESTIMTE IS 68 THE 95% CONFIENCE INTERVAL IS 64 71
STEELHEAD FRY (215 HOURS)
COPPEP1ZI K (COPPER)
SEPVEMBER 16e 1982 DILUTERS B 1 9 105 TRIMED SPEARMlH@RSER ANALYSIS THE DOSES (PARTS PER BILLION) ERE 18 30 47 49 57 73 78 86 113 134 THE NUMBER OF FISH IN RESPECTIE Tale ( 30 30 30 30 30 30 30 30 30 30 TE PROPORTION KAD IN 96 H0tRS ERE 0.0000 0.0333 0.0333 0.0333 0.2000 0.6000 0.8667 0.9667 1.0000 1.0000 THE SPEARMAN KAR9ER LC50 ESTIM TE IS 67 VE 951 CONFlKKE INTERVAL IS 64 71
ms_
p STEELEAD FRY (46 HOURS)
SEPTEM K R 16s 1992 r DILU1C93 8 1 0 _
10% TRIMMED SPEMMN-KARER ANALYSIS H!F TE DOSES (PARTS PER BILLION) ERE 81 108 148 140 174 215 266 273 349 385 TE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 y THE PROPORTION K AD IN 96 HOURS E RE 0.00 H 0.0000 0.0000 0.0000 0.0333 0.1667 0.4000 0.6333 0.9333 0.9667 E i_
THE SPEARMN KARBER LC50 ESTIMTE IS 265 g.
TE 95% CCNFlKNCE INTERVAL IS 251 279 2
?
w STEELHEAD FRY (53 HOURS)
SEPTEMER 16,1982 -
DILUTERS B 1 D C' 1)! TRIMED SPEARMAN-KARBER ANALYSIS THE DOSES (PAP.TS PER BILLION) ERE B1 108 148 140 174 215 266 273 349 385 E 30 30 30 30 30 30 30 30 30 30 THE NUMBER OF FISH IN RESPECTIVE TANK T E PROPORTION DEAD IN 96 HOURS K RE 0.0000 0.0000 0.0000 0.0000 0.0333 0.2667 0.6000 0.8000 0.9333 1.0000 g THE SPEARMAN KAR K R LC50 ESTI MTE IS 244 TE 95% COWIDENCE INTERVAL IS 231 258 g STEELHEAD FRY (70 HOURS) g COPPER / ZINC (ZINC) L SEPTEMBER 16,1982 DILUTERS B 1 D -
10% TRIMED SPEARMAN-KARER ANALYSIS THE DOSES (PARTS PER BILLION) ERE 81 108 113 140 174 215 266 273 349 385 ~
~
TE NUMER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION KAD IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0000 0.0333 0.4000 0.8000 0.90001.00001.00M
=
THE SPEARMAN KAR ER LC50 ESTIMATE IS 226
=
THE 95% CONFIKMCE INTERVAL IS 214 238 I
STEEUDD FRY (78 HOURS) _
SEPTEMER 16,1982 DILUTERS B 1 0 =
10% TRIMMED SPEARMAN-KARBER ANALYSIS -
TE DOSES (PARTS PER BILLION) ERE 81 100 148 140 174 215 266 273 349 385 TE NUMER OF FISH IN RESPECTIVE TAE 30 30 30 30 30 30 30 30 30 30 l THE PROPORTION KAD IN 96 HOURS ERE 0.0000 0.0000 0.0000 0.0333 0.1000 0.4333 0.8000 0.9667 1.0000 1.0000 =
TE SPEARMAN KARKR LC50 ESTIMTE IS 222 THE 95% CONFIDENCE INTER"AL IS 210 234
STEELHEAD FRY (94 HOURS)
COPPER /ZIE (ZIE)
S&TEMER 16,1982 DILUTERS B 1 0 10% TRIMER SPEARMN-KARBER AMLYSIS THE DOSES (PARTS PER BILLION) UERE 31 108 148 1M 174 215 264 273 349 385 TE NUMER OF FISH IN ESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION BEAD IN 96 HOURS K RE 0.0H0 0.0000 0.0000 0.0333 0.1 M7 0.5000 0.8M0 0.9M7 1.0000 1.0000 TE SPEARMAN KARBER LC50 ESTIMTE IS 215 TE 95% CONFIKKE INTERVAL IS 202 229 STEELEAD FRY (100 HOLRS)
SEPTEMKR 16e 1982 DILUTERS B 1 D 10% TRIMMED SPEARMA H ARBER AM LYSIS THE BOSES (PARTS PER BILLION) ERE 81 108 148 140 174 215 266 273 349 385
-THE NUMBER OF FISH IN RESPECTIVE TAE 30 30 30 30 30 30 30 30 30 30 THE PROPORTION KAB IN 96 HOURS ERE 0.0000 0.0333 0.0333 0.0333 0.1M7 0.50M 0.8667 0.9M71.00001.0000 THE SPOWtMAN KARBER LC50 ESTIMTE IS 213 TE 95% C0lFIENCE INTERVAL IS 201 227 STEELEAD FRY (124 HOURS)
CDPPER/ZIE (ZIE )
SEPTEMER 16,1982 BILUTERS B 1 D 10% TRIMED SPEARMAN-KARBER ANALYSIS TE DOSES (PARTS PER BILLICN) WERE 81 108 148 140 174 215 2M 273 349 385 THE NUMBER OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 T E PROPORTION DEAD IN 96 HOURS WERE 0.0000 0.0333 0.0333 0.0333 0.20M 0.5333 0.8667 0.96671.0M01.0M0 TE SPEARMAN KARER LC50 ESTIMATE IS 210 THE 95% CONFlKKE INTERVAL IS 197 223 STEELEAD FRY (167 HOURS)
SEPTEMER 16,1982 DILUTL S 31 D 10; TRindD SPEARMA H ARE R ANALYSIS TE BOSES (PARTS PER BILLION) ERE 81 108 148 140 174 215 264 273 349 385 THE NUM KR OF FISH IN RESPECTIVE TANK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION MAD IN 96 HOURS E RE 0.0000 0.0333 0.0333 0.0333 0.20M 0.5M7 0.8M7 0.96671.00001.M00 TE SPEARMAN KARBER LC50 ESTIMATE IS 208 TE 95% CONFIDEEE INTERVAL IS 195 221
l l STEELHEAD FRY (215 HOW S)
S&TENKR 16,1982 DILUTEPS B 1 D 10% TRIMMED SPEARMHARBut ANALYSIS THE DOSES (PARTS PER BILLION) WDtE 81 108 148 140 174 215 266 273 349 385 THE H m BER OF FISH IN RESPECTlYE TANK 30 30 30 30 30 30 30 30 30 30 THE PROPORTION BEAD IN 96 HOURS ERE 0.0000 0.0333 0.0333 0.0333 0.2000 0.6000 0.8667 0.9667 1. 4 1.0000 THE SPEARMAN KARIER LC50 ESTIMTE IS 206 TE 95% CONFIDENCE INTERVAL IS 194 219 l
m l
l l
l
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