ML20079N211

Environ Monitoring Program 1977 Annual Rept 1977 Attached Algae Studies of Mississippi River Near Monticello Nuclear Generating Plant

ML20079N211
Person / Time
Site:	Monticello
Issue date:	12/31/1977
From:	Kuhl G NORTHERN STATES POWER CO.
To:
References
RTR-NUREG-1437 AR, NUDOCS 9111110114
Download: ML20079N211 (42)

Text

-__-_____ _ _ _ _ _ _ _ _ _ _ _ - _ _ __

1. HONTICLLLO NUCLEAR GEd PLANT

, ATTACHMLt'T 2 MONTICELLO NUCLEAR GENERATING PLANT ENVIRONMENTAL MONITORING PROGRAy 1977 ANNUAL REPORT 1977 ATTACHED ALGAE STUDIES OF Tjg JiISSISSIPPI RIVZR NPAR THE MONTICELLO NUCLEAR GENERATING PLANT (2.1)

For Northern States Power Company Minneapolis, Minnesota by G. M. Kuhl Environmental and Governmental Activities Department Northern States Power Company i

9111110114 771231 t

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e 2.1 1977 ATTACHED ALGAE STUDIES OF TIIE MISSISSIPPI RIVQ NEAR THE MONTICELLO NUCLEAR GENERATING PIRU P

2.

1.1 INTRODUCTION

i Attached algae data gathered during it /7 represented the ninth year of the program with seven operational years and two preoperational years of data. The periphyti..: flora of large rivers is found on or near the substrate of the river-bed, aquatic macrophytes, and various types of debris. Algal colls sloughed or abraded off their natural substrates are available for colonization onto glass microscope slides utilized in the monitoring program.

This report it *Stigates seasonal trends of the periphyton community in response to natural environmental factors and ths addition of thermal plant effluent. The interaction of seasonal data', based on current velocities and temperatures, allows statistical analysis to indicate the presence or absence of significant environmental impact. Species composi-tion and cell biomass estimates, coupled with the statistical analysis, provide the basis for interpreting the periphyton community.

2.1.2 , MATERIALS AND MET!!ODS 2.1.2.1 Field Procedurou Five periphy'.on monitoring stations are located within the study area sFigure 2.1-1). Stations 2 and 7 represent ambient locations, vnereas Stations 3, 37., and 11 are affected by the thermal plume. Field sampling procedures remained consistent 2.1-3

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with previous years. Nine glass microscope slides were  ;

placed in a stainless steel and acrylic plastic sampler and allowed to colonize in the river for 14 days (Figure 2.1-2).

Af ter the colonization period, one slide was removed from each rung, the side not facing the current scraped clean, and then placed in a bottle containing approximately 10 ml of magnesium carbonate saturated 90 percent aqueous acetone Ii

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solution. The remaining slidns were placed in order in an j appropriately labeled slide box. All samples were placed in an ice chest and returned to the laboratory.

2.1.2.2 laboratory Procedures 2.1.2.2.1 Pigment Analyses Pigment analysis procedures for chlorophyll a remained consistent with previous yearst samples were scraped, macer-ated, diluted to a known volume (25-100 ml), and centrifuged for ten minutes. Pigment extract was decanted into a four-centimeter cuvette and analyzed at 665 nm and 750 nm before and after acidification using a Beckman Model DB-GT spectro-photometer. Chlorophyll a concentrations and Phaeophytin 3, the primary degradation product of calorophyll a,, were deter-mined by computer program utilizing the Lorenzen (1967) equations ,

Chlorophyll a ug/cm = 26.7 E(665-750)" - (665-750)*3x V ,

(18.0 cm / slide) (4 cm light path)

Phaeophytin a ug/cm = 16. 7[1.7 (665-750)" -(665-750)"] x v .

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. where u = unacidified a = acidified 665 = Chlorophyll a_ absorbance 750 = turbidity blank absorbance V = acetone & chlorophyll a_ extraction volume (ml)

Results of above computer analysis are expressed as micro-grams of chlorophyll,a,and phaeophytin a per square centi-meter, and as percent chlorophyll a (chlorophyll a_ x 100/

chlorophyll a + phaeophytin a_) .

As in previous years, a three-way arr.lysto of variance on chlorophyll a data evaluated the significance of interaction between ambient Stations 2 and 7, and heated Stations 3 and 11 (Knutson 1976). Stations 2 and 11 woro paired as medium current velocity; and Stations 3 and 7 were paired as having fast current velocities. The F-test values consider tempera-ture (ambient versus heated), time, and habitat.

2.1.2.2.2 Taxonomic Identification and Enumeration Periphyton enumeration was carried out utilizing two methods, one method for non-diatom species and another method for analyzing diatoms. Non-diatom species were mounted using the setme'methnd employed by Knutson (1976). Diatoms were prepared by scraping the algal film from the slide into a 250 ml beaker and cleaned using nitric acid and potassium dichromate. Cleaned frustules were placed in a vial and diluted to ten milliliters with distilled water. This sample was then shaken, and a known aliquot pipetted onto a 2.1-5

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22 mm No. 1 cover glass. The cover glass was heated on a hotplate to evaporate all moisture and a permanent mount made using Hyrax. The finished slide was labeled and categorized until identification and enumeration analyses.

Enumeration procedures were done using an American Optical Phase Star phase contrast microscope and 1000 x oil immersion.

etendard taxonomic keys were used to identify algal species observed on 30 random fields or until 100 of the most dominant species were tallied. Raw data were then extrapolated to determine algal density (cells /squore entimeter).

2.1.3 RESULTS 2.1.3.1 Physical Parameters The 1977 sampling year ran from April 13 through November 10 with 15 sampling periods averaging 14 days each. Table 2.1-1 shows the sampling schedule, mean river water discharge, mesn ambient river water temperature, and plant off-line times.

River _ water temperrture data were taken at each station on each sampling date. Temperatures ranged trom 7.0 C to 27.5 C at ambient stations, whereas heated stations had a temperature range from 8.0 C to 33.0 C. Extended' periods of plant off-line time resulted in heated station temperatures approaching ambient river water temperatures.

Current velocity measurements were taken at each station on all dates, with the exception of periods of instrument mal-2.1-6

0 function. Current velocity, related to river water discharge, exhibited a range from 0.0 meter per second at Station 3A in the discharge canal during periods of plant off-line to a maximum of 1.2 meters per second at Station 2 on April 26 and June 7. Seasonal mean current velocities were 0.9 and 0.6 meters per second for ambient Stations 2 and 11, respectively, while heated Stations 3 and 11 exhibited a acasonal mean of 0.6 meter per second for both stations.

2.1.3.2 Chlorophyll a Analyses Tablo 2.1-2 shows chlorophyll a concentrations (micrograms per square cantimeter) for all stations on all sampling dates.

Ambient stations had a chlorophyll a range from 0.17 ug/cm at Station 2 on October 12 to 7.89 ug/cm at Station 2 on April 26. Heated stations had chloropi.yll a ranges from 0.07 pg/cm at Station 11 on October 26 to 7.59 ug/cm at Station 7 on April 26. nigh spring chlorophyll & concentra-tions at both heated and ambient stations are associated with the spring diatom community of Diatoma vulgare, Navicula salinarum var. _ intermedia, and Gomphonema olivaceum.

Figure 2.1-3 shows the seasonal chlorophyll a concentrations for all stations. Spring maxima are followed by irregular summer trendJ. Fall chlorophyll a concentrations drop before the onset of the late fall / winter community. Figures 2.1-4 through 2.1-8 indicate chlorophyll a concentrations related to river water temperature and current velocity.

Table 2.1-3 shows seasonal percent chlorophyll a values for all stations. Ambient stations exhibited a range from 54 2.1-7

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percent at Station 7 on May 24 to 100 percent at Station 7 f on April 26. Heated stations had a percent chlorophyll a i range from 39 percent at Station 3A on June 7 to 100 percent at Stations 3 and 11 on November 9. Seasonal means for per- i cent chlorophyll a values were 87 percent for ambient Stations .

2 and 7 and 76 percent for heated Stations 3, 3A, and 11. l I

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Table 2.1-4 shows phaeophytin a corqentratione for all sampling periods at all stations. Ambient stations had a ,

range from 0.00 ug/cm at Station 7 on April 26 and November 9 to 1.31 ug/cm at Station 7 on May 24. Heated stations had a phaeophytin range from 0.00 ug/cm at Stations 3 and 11 on November 9 to 1.85 ug/cm at Station 3A on June 7.

2.1.4 DISCUSSION The attached algae community of the Mississippi River near Monticello, Minnesota has had considerable investigation since 1969. During this time the species composition and chlorophyll a biomass estimates have defined the qualitative and quantitative aspects of the periphyton assemblage.

-Addition of thermal effluent han been the area of major interest in relation to possible adverse environmental impact.

A three-way analysis of variance (ANOVA) was conducted on chlorophyli a biomass estimates relative to time, temperature, habitat,- and their interaction components. No significant dif ference was shown with respect to all statistical components of the ANOVA with the exception of time. Th.s is an expected response due to the population fluctuations from one season to another. This does not mean that heat has no effect on ,

the algal community, but rather the effect is below the

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e statistical level of significance.

Seaconal trands of chlorophyll a biomass during 1977 are representative of previous years with comparable flow and tamperature regimes. The high spring peak in chlorophyll a corresponds to the spring diatom community of Diatoma vulgare, Navicula salinarum var. intermedia, and Gomphonene olivaceum.

This peak, found only in ambient stations, was followed by a small uummer peak. The onset of the fall-winter diatom assen;blages is evident in a rise in chlorophyll a biomass shortly before the termination of the study.

Species cotaposition data gathered during 1977 reflected close-similarity with those of previous years' data, indicating no change in species dominance from the thermal effluent.

Cocconcis placentul.a, Diatoma vulgare, Gomphonoma olivacnum, Gomphonema parvulum, and Navicula salinarum var, intermedia remained the most stable elements of the diatom cor.cuunity.

Chroococcus minimus and Phormidium faveplarum were dominant members of the Cyanophyta (see Table 2.1-5 for species list) .

The periphytic community studied for the last nine yt*Crs represents an important elemene in the trophic structure of the river. Any severe impact to the algal community would l'

probably,be evidant in successive elements of trophic levels.

The ability of these primary producers to recover in a short period of time has resulted in a stable population since 1969. During this time, there has been no evidence of detri-mental environmental impact on the periphyton community.

The lack of adverse effects supports the deletion of the Monticello Nuclear Generating Plant Periphyton Program.

2.1 9

)

2.1.5

SUMMARY

1. Three-way ANOVA statistics showed no significant effect on chlorophyll a biomass resulting from thermal effluent addition.

2. Species composition of the Mississippi River near Monti-cello, Minnesota remained unchanged in 1977 with respect to species dominance and seasonal population trends.

3. Chlorophyll a biomass trends during 1977 were representa-tive of previous years of comparu le temperature and flow.

4. During the nine years of periphyton monitoring, no adverse environmental effects have been detected.

2.1-10

i I 2.1.6 LITERATURE CITED I

Knutson, K. H. 1976. Attached Algae Studies in the Missis-sippi River at Monticello During 1976. J_N Environmental Monitoring and Ecological Studies Program, NSP Annual Report 1976.

Lorenzen, C. J. 1967. Determination of Chlorophyll and Pheo-pigments: Spectrophotometric Equations. Limnol. Oceanogr.

12(3)3343-346.

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Ten.pe ratur e , and Current Velocity at Station 11 _

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- W,Y JUN- JUL AUG SEP OCT NOV DATE 2.1-20

I Table 2.1-1 1977 Sampling Schedule, Mean Ambient River Water Temperatures, Mean River Discharge, and Plant Off-Line Time.

Mean River Water Mean Ambient River Plant Off-Line Time Sampling Periods Days Discharge a(cfs) Water Temperatures"( c) Days Date

1. 13 April-2C April 13 3396 13.4 0.5 19 April

2. 26 April-09 May 13 3174 16.1

3. 09 May-24 May 15 2023 21.2 y' 4. 24 May-07 June 14 2321 23.1 u

5. 07 June-22 June 15 2022 21.8 3.7 10 June-14 June

6. 22 June-06 July 14 2247 24.1 2.2 25 June-27 June

7. 06 July-20 July 14 2763 25.5

8. 20 July-03 August 14 1771 24.6

9. 03 August-17 August 14 1325 22.2

10. 17 August-31 August 14 1303 20.5

11. 31 August-14 September 14 3584 19.6

12. 14 September-28 September 14 4002 16.7

13. 28 September-12 October 14 5400 12.8 09 S Ptember-

> 62.0 10 November s-

i' I

} . Table 2.1-l' 1977 Sampling Schedule, Mean Ambient River Water Reaperatures, Mean River Discharge, and e

l Plant Off-Line Time '(continued). ,

1 I

i ..

! Mean River Water Mean A5bient River Plant Off-Line T1sme

• 1 Sampling Periods Days Discharge a(cfs) Water Temperatures *("c) Days Date

-e ,

i .[

14. 12 October-26 October 14 5580' 8.3

~

- . 15. 26 October-10 Novemmber 14 5399 9.1  !

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u l i

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N +

i aData obtained frous Monticallo Nuclear Generating Plant Computer readout s

4

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Table 2.1-2 1977 Chlorophyll a Concentrations at Each Station Expressed as ug/cm2 / sampling period 1

Collection Date Station 2 3 3A 7 13 26 April 7.89 6.22 0.68 7.59 2.31 09 May 0.99 1.00 0.68 0.98 1. 7.8 24 May 2.84 ---- 0.,92 1.53 5.42 07 June 0.88 0.93 1.16 0.91 1.00 22 June 1.21 1.65 1.67 1.83 1.41 06 July 0.94 1.56 0.95 0.85 1.15 20 July 0.87 0, ?' l.37 1,66 1.06 03 August 0.70 0.41 0.22 2.61 1.87 17 August 1.13 0.86 1.16 3.02 ----

31 August 1.98 0.49 0.39 2.68 2.10 4 September 0.28 1.09 0.54 2.04 1.55 28 September 0.33 0.74 1.29 1.03 0.49 12 October 0.17 0.22 0.91 -0.26 0.23 26 October 0.24 0.21 0.29 0.18 0.07 09 November _1.48 2.18 0.46 1,51 0.64 Seasonal Mean 1.47 1.32 0.85 1.91 1.51 l

l 2.1-23

. . . _ . . - - . . . ~ . __. _._ ._ _ . _ . _ _. _ ._ _ _ ._ ._.- ._._.__.m__._

Table:2.1-3 ~1977 Percent Chlorophyll i Values for Each Station. Percent Chlorophyll i=

- chl 1-x 100 Chl' A + Phao 1 i

Collection Date Station 2 3 3A~ 7 11

-26 April. 99 92 54 100 92 09 May 74 81 42 86 89 24 May- 88 --

48 53 94 * -l

07 June 80 81 38 85 80 i

22 June 90' 82- '71 94 83 06 July- 84 79 76 95 - 89' 20.-July 93 73 59 96 92 03 August 88 66 .64 96 92 August 79' '87 -68 92 --

~31 August- 96- 65 57 -91 83 14' September-63' 84 66 93 96 28-September 88 83 84 85 84

12. october- -72 84' 84 _ 87 90- .: l; g

26; October 89- 98 '81 75 58-i.

09 November- ,98 - 100 8_q ' 100 100 Seasonal Mean. 85 82 '

65 188 81

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, 2.1-24 I

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9 Table 2.1-4 1977 Phaeophytin a, concentrat. is at Each Station Expressed as pg/cm2/ sampling period Collection Date Station 2 3 3A 7 'A 11 26 April 0.07 0.52 0.56 0.00 0.19 09 May 0.35 0.24 0.92 0.16 0.21 24 May C.39 ----

0.99 1.31 0.30 07 June 0.12 0.22 1.85 0.16 0.25 22 June .13 0.36 0.67 0.10 0.28 06 July s.18 0.41 0.29 0.04 0.14 20 July 0.07 0.34 0.95 0.06 0.09 03 August 0.10 0.21 0.12 0.10 0.15 17 August 0.29 0.13 0.52 0.23 ----

31 August 0.09 0.27 0.29 0.26 0.42 14 September 0.16 0.21 0.28 0.15 0.06 28 September 0.05 0.15 0.24 0.18 0.09 12 october 0.07 0.04 0.17 0.04 0.02 26 October 0.03 0.01 0.07 0.06 0.05 09 November 0.03 0.00 0.10 0.00 0.00 m n.

2.1-25

i Table 2.1-3. Attached Algae of the Mississippi River near Monticello Numbers Expressed as Cells per Square Centimeter 26 April 1977 Station 3A 7 11 Species 2 3 10,200 5,610 B iuarGLfLCD_ Algae 3 40,290 510 510 0 0 chroncoccus minimus 510 0 _

2,550 0

Phormidinm faveolarum 1,530 0 0 510 Spiru11na subtillissima 0 Green _ Algae 6,630 0 l 0 0 0 i Scenedesmus_sp. 39,780 0 0 0 f Stigeoclonium nanum 3,950 0 0 l Dtatoms 0 1,975 Achnanthos:=xigna 1,975 0 0 l 0

_. 0 3,950 Achnanthea_ lanceolata 0 1,975 l 1,975 0 3,950 u Asterionella formosa 9,875 1,975 0 7,900 9,875

• Cocecneis pediculus 51,350 11,850 5,925 l g 3,950 Cocconcis pla"entula var.

h euglypta 33,575 3,950 1,975 m 1,975 5,925 '

Cyclotolla meneghiniana 0 11,850 ~d '

31,600 1,975 Diarcma elongatum __ _ 17,775 9,675 7,900 31,600 3,950 Diatoma vulgare 33,575 110,600 13,825 l

67,150 7,900 Gomphonema olivaceum 3,950 1,975 80,975 0 0 Melosira granulata 31,600 1,975 0 0 5,925 Navicula gracilis 3,950 0 3,950 3,950 1,975 Navicula hungarica var. cap-1,975 0 5,925

! itata 41,475 1,975 Navicula salinarum var. i 1,975 intermedia 1,975 3,950 1,975 7,900 Nitzschia acicularis 5,925 0 3,950 3,950 0 ,

Mit2LgcI ia dissipata 0 1,975 0 l

9,875 0 ,

5,925 Nitzschia palea 1,975 25,673 1 975 0

Stephanodiscus niagarae

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Table 2.1-5. Attached Algae of the Mississippi River near Monticello Numbers Expressed as CelJs per Square Centimeter 09 May 1977 Station Species 2 3 3A 7 11

_Bluegreen Algae ~~

Chroococcus minimus 39,780 34,170 510 14,790 14,790 Oscillatoria sp. O C 510 0 0 _

Phormidium ' faveolartre 0 0 6,120 510 0 Green A10ae Characium pringsheimii 0 0 367,710 0 0 Stigecclonium manum 0 3,570 23,460 0 0 Diatoms ,

Achnanthes exiqua 0 0 1,975 0 't

" 0 1,975 3,950 0 1,975 Achnanthes lanceolata var.

7' omissa y Amphora perpusilla 1,975 0 0 1,975 0 Asterionella formosa 13,825 0 0 0 0 Cocconeis pediculus 19,750 5,925 0 1,975 0 Cocconels placentula var.

euglypta 5,925 51,350 9,875 3,950 5,925 Cyclotella meneghiniana 1,975 3,950 33,575 3,950 1,975 Diatoma'elongatum 23,700 0 7,900 25,675 7,900 Diatoma vulgare ,_

31,600 0 0 27,650 9,875 Fragilaria capucina 3,950 3,950 73,075 7,900 11,850 Gomphonema olivaceum 80,975 5,925 1,975 124,425 3,950 Gomphonema parvalum 5,925 51,350 73,075 1,975 3,950 Navicula gracilis 3,950 1,975 0 3,950 1,975 Navicula salinarum var. 1,975 3,950 23.700 5,925 0 intermedia

'Nitzschia acicularis O 1,975 5,925 3,950 0 Stephanodiscus niagarae 5,925 5,925 23,700 0 0 43,450 9,875 3,950 11,850 7,900 synedra actina steraides

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I Table-2.1-5. Attached Algae of the Mississippi River near Monticello

- Numbers Expressed as Cells per Square Centineter f 24 May 1977 Station j Species 3 3A  ! 7 11 2

Blue-Green Algae 6,630 46,920 214,710 33,660 163,710 Chroococcus minimtm 8,160 510 4.080 1.020 Phormidium faveolarum 8.670 0 510 3.060 spirulina r-htilissima 1.530 Green Alcae 510 1,530 0 0 Stiaeoclonium nanum 0 0 0 519 0 Closterium sp. 0

_ Diatoms _

0 0 5,925 5,925 1,975 Achnanthes lanceolata var. _

lanceolata ~

1,975 73,075 25,675 5,925 0

, Achnanthes minutissima 3,925 0 1,975 0 Cccccneio pediculus 3,950 7 17,775 0 13,825 67,150 1 Cocconeis placentula 75,050 g 199,475 161,950 1,975 280,450 Cocconeis placentula var.

euglypta 3,950 Cymbella ventricosa 0 ~0 0 19,750 1,975 1,975 0 1,975 Diatoma vulgare 23,700 0 3,950 25,675 0 Gomphonema angustatum 33,575 39,500 41,475 0 29,625 0 Gomphonema olivaceum -

0

~

35,550 41,475 100,725 238,975 Gomphanema parvulum 1,975 5,925 1,975 7,900 Navicula gracilis 33,975 1,975 0 3,950, O 15,800 Navicula salinarum var.

intermedia 0 5,925 0 f Nitzschia dissipata 13,825 0

) 0 1,975 15,800 1,975 l Nitzschia palea 5,925 1,975 3,950 17,775 0 0

__ _Hitrachia romana 1,975 1,975 3,950 3,950 0 nunnarn rumpenn 31,975 3,950 0 1,975 0 S'ynedra ulna b h 'heutS , he e D i , & gaan e, , , .,y p

_a m

w

Table 2.1-5. Attached Algae of the Mississippi River near Monticello Numbers Expressed as Cells per Square Centimeter 07 June 1977 Station Species' 2 3 3A 7 11 151ue-Green - Algae 510 3,060 0 0 Chroccoccus minimus O 10,200 510 O 1 1,020 1,020 Phormidium faveolar_um

_ Green Algae '

O O 22,440 0 0 l

Cnaracium pringsheimii

._ Diatoms 1,375 5,925 0 3,950 Achnanthes exigua 1,975 51,350 136,275 86,900 15,800 90,850 Cocconeis placentula

" 7,900 0 1,975 0 0 Diatma vulgare 77,025 0 3,950 33,575 0 7 comphonema elivaceum 77,025 15,800 41,475 43,450 0

$ Gsphonema parvalum 11,850 3,950 5,925 5,925 0 Melosira varians 1,975 1,975 5,925 Navicula hungarica 1,975 1,975 l

Navicula salinarum var. 3,950 1,975 21,725 0 0 intermedia O 1,975 0 0 0 Navicula exigua 1,975 0 0 3,950 0 Nitzschia palea 3,950 0 1,975 1,975 __ O Nitzschia ramana 0 5,925 Synedra rumpens 5,925 0 0 11,850 0 0 13,825 3,950 Svnedra ulna m

- rt

Ta51e 2.1-5. Attached Algae of the Mississippi River near Monticello Numbers Expressed as Cells per Square Centimeter 22 June 1977 Station Species 2 3 3A 7 11 Blue-Green Alga' 45,39C 10,710 51,000 24,990 10,710 Chroococcus mir.n Js 1,020 0 0 Oscillatoria amphibia 1,020 0 0 510 1,530 3,570 Phonmidium faveolarum 66,810 Green Algae 0 0 0 71,910 80,070 Characium pringsheimii Diatoms ~

1,975 7,900 0 1,975 13,825 Achnanthes lance 91sta 0 0 0 0 3,950 Achnanthes l i n e a_t_ i s 3,950 317,975 337,725 278,475 304,150 Cocconcis placentula_ 1,975 7,900 5,925 Cocconeis pediculus var.euglypta 33,575 3,950 1,975 0 7,900 1,975 13,825 l# Diatoma vulgare 15,800 0 35,550 47,430 Gomohopema olivaceum -

35,,475 Y 343,650 94,800 0 80,975 120,475 kl Gomphonelaa parvulum 0 5,925 0 3,950 5,925 Navicula salinarum var.

intermedia 0 0 1,975 Navicula gracilis 0 0 1,975 0 1,975 1,975 0 Nitzschia dissipata 3,950 0 3,950 0 3,950 dynedra ulna b

~

Table 2.1-5. Attached Algae of the: Mississippi River near Monticello Numbm a Expressed as Cells per Square Centimeter ,

06 July 1977 l Strtion Specie's 2 3 3A 7 11 Blue-Green Algae Chroccoccus minimus 109,140 24,990 116.280 1.020 510 Oscillatoria amphibia 0 1,530 8.670 1.530 1_530 Phormidium faveolarum 10,710 7,140 32.640 2.040 1.530 Green Algae Characium pringsheimii 0 0 36,210 0 0 Stigeoclonium nanum O 510 6.630 0 0 '

Diatons w- __ Achnanthes exiqua 0 5.925 7.900 0

, 1_479 r, Achnanthes lanceolata 1,975 0 1.975 1 325 3.950 b

Achnanthes lanceolal= var. 61,225 395,000 35.550 61,275 3.950 omissa i Cocconeis placentula 219,225 33,575 3,950 195,525 1,975 Cymbella ventricosa 7,900 1.975 0 9,875 0 Gomphonema parvulum' 7,900 11,850 0 5,925 31,600 Gomphonema parvulum var. 23,700 80,975 0 19,750 9,875 micropus Navicula hungarica 1,975 0 1,975 1,975 1,975 Navicula rhyncocephala 1,975 0 0 3,950 3,950 Nitzschia palea O 5,925 7,900 11.850 13.825 Synedra rumpens 1,975 3,950 0 5.925 0 l

u Table 2.1-5. Attached Algae of the Mississippi River near Monticello Numbers Expressed as Cells per Square Centimeter 20 July 1977 Station Species 2 3 3A 7 11 Blue-Green Algae Chroccoccus minimus 30,090 102,000 76,500 4,590 71, 4 0 f.-

Phormidium fav'osum 2,040 510 3,570 0 1,530  !

Phormidium faveolarum 7,140 16,830 14,790 1,530 45,900 l Green Algae j l

Characium pringsheimii 0 1,020 122,910 0 closterium sp. 510 0 0 0 Diatoms Achnanthes lanceolata var. S'.,350 80,975 1,975 0 159,975

~

omissa Achuanthes l_icearis 11,850 1,975 1,975 0 0 g

• Cocconeis placentula 237,000 355,500 0 67,150 1,975 g

Cymbella centricosa 1,975 0 0 5,925 1,975 6 0 3,950 3,950 A Gomphonema olivaceum _

3,950 0 Gomphonema parvulum 7,900 0 0 1,975 1,975

~

Gomphonema parvuluta var. micropusl5,8CO 238,975 0 1,975 1,975 Melosira varians _0 3,950 3,950 1,975 3,950 Navi cula salinarum var. 3,950 5,925 0 3,950 5,925 intermedia Nitzschia palea 7,900 0 0 5,925 0 Nitzschia romana 13,825 1,975 0 5,925 0 i

^

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. - _ - - - - , - - - ' i

- ~

Table 2.1-5. Attached Algae Of the Mississippi River near Monticello Numbers Expressed as Cells per Square Centimqter 03 August 1977 i

Station Species- I 2 3 3A 7 11 Blue-Green Algae Calothrix membranacea 0 510 0 61,710 0 Chroococcus minimus 102,000 95,880 95,880 0 107,100

~

Phormidium favosum 3,570 0 16,320 0 0 Phormidium faveolarum 78,540 3,570 20,910 3,060 44,880 Green Algae Closterium sp. 510 0 0 0 0 m

. Diatoms .

i g Aclinanthes exigua l O 1,975 17,775 0 0 t i Achnanthes lanceolata var. 17,775 3,950 5,925 3,950 57,275

• lanceolata .

i, Achnanthen lanceolata var.- '

omissa 9, 8'/ 5 1,975 0 1,975 120,475 Cocccneis placentula 278,475 371,300 13,825 357,475 363,400 Cymbe1la tumida 3,950 0 1,975 0 1,975 -

Gomphonema parvulum 7,900 0 23,700 0 349,575 Gomphonema parvulum var. micropus 86,900 0 37,525 0 33,575 Navicula exigua 1,975 3,950 0 1,975 0 Navicula salinarum var. 5,925 1,975 0 3,950 3,950 intermedia riitzschia palea 5,925 3 , 9 97 7,9GC 0 0 Nitzschia romana__ 1,975 0 3, EG _

3,950 0 Synedra ulna var. O O O 1,975 3,925 oxyrl' chus _

. m - /

Table 2.1-5. Attached Algae of the Mississippi River near Monticello Numbers Expressed as Cells per Square Centimeter 17 August 1977 l Station Species 2 3 3A 7 11 Blue-Green Algae ,

Chroococcus minimus 124,440 140,410 71,910 41,310 107.100 Phormidium favosum 2,040 0 0 0 0 Phormidium faveolarum 10,710 1,530 0 0 7.140 Green Algae Stigeoclonium nanum 0 510 510 0 0 Diatoms Achnanthes lanceolata var. 0 1.975 3.950 0 1.975 lanceolata Achnenthes lanceolata var.

w omissa 23,700 1,975 17.775 1.975 0 11,850 0 19,750 3.950 17,775 e Achnanthes linearls 5,925 3,950 1,975 7.900 3.950 b Cocconeis pcdiculus 317,975

• Cocconeis placentula 225,150 237,000 179,725 120,475 Cymbella tumida 1,975 0 3,950 0 1,975 Diatoma elongatum 1,975 0 0 0 0 Gomphonema olivaceum 7,900 0 0 1,975 0 0 1,975 15,800 3,950 Gomphonema parvulum var. 0 _

micropus

~

Navicula salinarum var- 3,950 3 1 950 3,950 0 5,925 intermedia Nitzschia palea 3,950 5,925 0 1,975 0 Nitzschia <omana 1,975 3,950 0 0 0 0 1,975 0 1,975 3,950 Synedra ulna l

+,., . . n.. . ..

- -M

Table 2.1-5. Attached Algae of the Mississippi River near Monticello Numbers. Expressed as Cells per Square Centimeter 31 August 1977 Station Species ~

2 3 3A 7 11 Blue-Green Alaae Chroococcus minimus 128,010 61,710 133,110 108,120 95,880

__ 'Phormidium favosum 0 0 2,040 0 0 Phormidium faveolarum 16,830 0 3,060 0 0

-Green Algae Stigeoclonium nanum 1,530 510 0 0 510

_ Diatoms Aci:nanthes lanceolata var. 3,950 0 75,050 0 3,950 m ianceolata g Achnanthes lanceolata var. 21.725 3.95Q_ 41.475 0 3.950

$ omissa  !

Achnanthes linearis 1.975 1.975 13,825 1,975 5,925 Cocconeis pediculus 3.950 0 0 3,950 0 l Cocconeis placentula 73,075 238,975 258,725 240,950 317,975

! Cymbella tumida 1,975 0 0 1,975 1,975 Epithem 16 sorex 0 1,975 3,950 0 0 Gombhonema olivaceum -61,225 0 0 0 1,975 l Gomphonema parvulum 27,650 0 0 0 3,950 Melosira varians 15,800 3,950 1,975 -

3,950 5,925 Navicula salinarum var. 11,850 0 0 5,925 intermedia 0 Nitzschia dissipata 5,925 1,975 1,975 1,975 0 Synedra ulna 1,975 0 0 3,950 0 J

Table 2.1-5. Attached Algae.of the Mississippi. River near Monticello Numbers Expressed as Cells per Square Centimeter 14 September 1977 Station Species 2 3 3A 7 11 Blue-Green Algae 95.880 10.710 108.120 22.440 71.910 Chroococcus minimus 1.530 0 1.020 2.040 Phormidium favosum 1.530 1.020 10.710 1.020 5.100 Phnrmidium faveolarum 2.040 510 0 0 0 0 Spirulina subtilissima Green Algae 0 510 0 510 0 Stiaeoclonium nanum ,

_piatoms 1.975 0 33.575 O 7.900 Achn an th e s lanceolata var. 4 fancentata 0 55.300 0 3.950 1.975 g

Achnanthes lanceolata var. l

+ omissa 55,300 23,700  !

13,825 53,325 0

Cocconeis pediculus 337,725 341,675 l 284,400 114,550 110,600

$ Cocconeis placentula 0 82,950  !

5,925 238,975 0 Gomphonema parvulum 3,950 0 317,975 7,900 55,300

-Gomphonema parvulum var.

micropus 0 1.975 0 0 0 Navicula decussis 0 0 3,950 0 Navicula qtstrum 3.950 l 3,950 0 0 1,975 Navicula s-linarura var, 5,925 __

f l intermedia 41,475 5,925 1,975 7,900 1,975 Nitzschia fonticola 3.950 1.975 1.975 0 1.975 Rhnicosphenia curvata O

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Tab'.y 2.l-5. . Attached Algae of the-Mi.ssissippi River near Monticello Numbers' Expressed as cells _per Square Centimeter 20 Septer*'oer - 1477 Station Species 2 3 3A 7 11 Blun-Grogn Algae Anahamna_sp. 51') O 510 1.020 0 chroncoccus minimus 1.020 0 15.810 1.020 0 Pharmidium_faveolarum 1,5]O 510 1.020 0 0 Green Alcae Characium orinosheimii O O 20,910 0 0 Diatoms Amphora ovalis 0 -0 0 3,950 0 g coccongis_ pediculus 5.925 7 200 7.900 1.975 3,950 Cocconsin placentula 73.075 77 J2 5 13.825 35.550 11,850 Y cocconeis plagentula var. '231700 5.925 17.775 55.300 0

$ eucivota i Cymbella tumida 0 1.975 11,850 0 0

Diatoma vulgare 33,575 27,650 23,825 1,975 _23,700 Pragilaria leptostauron 3,930 1,975 O O 1,975 Gomphonema olivaceum 17,775 0 0 1,975 1,975

) Gomphonema parvulum 3,950 1,975 1,975 3,950 3,950 Gomphonema parvulum var. 5,925 13,825 3,950 3,950 5,925 micropus Melosira varians 7,900 0 1,975 13,825 11,850 Hayigula_graeilis 0 1.915 9,875 11,850 9,975 Navicula salinarum var. O 7,900 27.650 13,825 41.475 intermedia Hitzschia amphibia 3,950 0 0 0 0 Syngdra ulna .- 1,975 1,975 0 3,950 5,925 y .- u

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Table 2.1-5. Attached Algae of the Mississippi River'near Monticello Numbers Expressed as Cells per Square Centimeter 26 October'1977 Station Specied 2 3 3A 7 11 No Quantitative Samples Due to Extremely Low Cell Densities I

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- 'I

Table 2.1-5.

Attached Algae of the Mississippi River-near Monticello Numbers Expressed as Cells per Square Centimeter 09 November 1977 Station 3A 7 11 Species 2 3 Blue-Green Al9ae 0 0 10.710 510 0 0 Chroccoccus minimus O O 3.060 0 Phormidium faveolarum Green Algae 20.400 0 0 0 0 Characium pringsheimii Diatams 1,975 3,950 3,950 1.975 3.950 ._

Cocconeis pediculus 7.900 3.950 0 Cocconeis placentula 3.950 13.825 1.975 O 3.950 _

Cymbella_tamida 1.975 O 27.650 - 31.600 25.675 23.700 11.850 Diatoma elongatum 80,973 33.575 55.300 41.475 100,725 Diatoma vulgare 0 1.97 5 1.975 g

Gomphonema parvulum 7.900 3.950 3.950 1,975 0 1.975 Gomphonema parvulum var. 3.,250

$ micropus O n 0 1.975 O NaYicula exigua ?_3,2nn 1_qsn 1.950 1_q7s 11__ B 2 5 l Navicula oracilin 5,_225 13.825 23.700 1.975 1.975 Navicula salinarum_ var.

intermedia 1,975 0 19,750 0 0 l Nitzschia acicularis 5.925 1.975 3.950 0 Nitzschia_pglga 5.925 3.950 1.975 0 5.925 0 1.975 Synedra ulne 7.900 5.925 5.925 Synedra ulna var.

1.975 oxyrhynchus

^

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-