ML20062D703

Quarterly Rept Bailly Nuc-1 Site Encompassing July-Sept 1978. Rept Contains Ecological Monitoring Data Necessary for Assessing & Minimizing Effects of Plant Constr

ML20062D703
Person / Time
Site:	Bailly
Issue date:	10/15/1978
From:	NORTHERN INDIANA PUBLIC SERVICE CO.
To:
References
NUDOCS 7811290178
Download: ML20062D703 (289)

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l-8 Density, Dominance Frequency, and Importance Values for 1-14 Vegetation Sampled in Cowles Bog Community, Location 4B, Bailly Study Area, July 1978 1-9 Density, Dominance, Frequency, and Importance Values for 1-16 Vegetation Sampled in Cowles Bog Community, Location 5, Bailly Study Area, July 1978 1-10 Density, Dominance, Frequency, and Importance Values for 1-17 Vegetation Sampled in Maple Forest Comminity, Location 6, Bailly Study Area, July 1978 1-11 Density, Dominance, Frequency, and Importance Values for 1-18 Vegetation Sampled in Emergent Macrophyte Community, Location 7, Bailly Study Area, July 1978 1-12 Density, Dominance, Frequency, and Importance Values for 1-18 i Vegetation Sampled in Transmission Corridor, Location 8, Bailly Study Area, July 1978 1-13 Vegetational Taxa Observed in Sedge Meadow Community, 1-19 Location 9, Bailly Study Area, July 1978

)

viii. solence senloos alvision I

TABLE OF CONTENTS (Contd)

TABLES (Contd)

Table Title Page 1-14 Vegetational Taxa Observed in Immature Oak Forest Community, 1-20 Location 10, Bailly Study Area, July 1978 1-15 Vegetational Taxa observed in Wetland Meadow Community, 1-21 Location ll, Bailly Study Area, July 1978 1-16 Mean Soil Conductivity Values from 10 Locations, Bailly 1-22 Study Area, May, July, 1978 1-17 Number of Mammals Reported from 8 Sampling Locations at the 1-22 Bailly Study Area, July 1978 1-18 Number of Amphibians and Reptiles Reported from 8 Sampling 1-25 Locations at the Bailly Study Area, July 1978 1-19 Checklist of Entomological Taxa Collected in the Bailly 1-28 Study Area, July 1978 2-1 Status of Laboratory Analyses as of October 1978, Bailly 2-2 Study Area 2-2 Dates and Purposes of All Aquatic Field Trips, Bailly Study 2-2 Area, Summer Quarter 1978 2-3 Phytoplankton Density, Bailly Study Area, June 1978 2-7 2-4 Phytoplankton Biovolume, Bailly Study Area, June 1978 2-15 2-5 Chlorophyll a Concentrations Recorded from 15 Phytoplankton 2-24 Sampling Stations in the Bailly Study Area, April 1978 2-6 Carbon Fixation Recorded at 15 Sampling Stations in Bailly 2-25 Study Area, June 1978 q 2-7 Periphyton Density, Bailly Study Area, June 1978 2-26 2-8 Periphyton Biovolume, Bailly Study Area, June 1978 2-32 2-9 Relative Abundance of Periphyton Diatoms at 5 Stations in 2-39 I Bailly Study Area, June 1978 1

2-10 Periphyton Chlorophyll a Concentrations Recorded from 2-40 Periphyton Samples in Bailly Study Area, June 1978 2-11 Zooplankton Density, Bailly Study Area, June 1978 2-42 ix solence servloes divleton

~ .

TABLE OF CONTENTS (Contd) .

TABLES (Contd)

Title Page i Table l

t Benthos Density, Bailly Study Area, June 1978 2-46 l 2-12 Benthic Particle Size Analysis in the Bailly Study Area, 2-51 2-13 August 1978 Macrophyte Composition, Bailly Study Area, Jitne 1978 2-54 -

2-14 A Generalized Key to the Common Nearshore Pond Macrophyte 2-56 2-15 Flora Collected in the Bailly Study Area 2-16 Fisheries Catch in Bailly Study Area, August 1973 2-58 2-17 Clip and Tagging Marks on Salmonids Collected in the Bailly 2-62 Study Area, August 1978 2-18 Ichthyoplankton Data, Bailly Study Area, June 1978 2-63 2-19 Incidental Ichthyoplankton Data, Bailly Study Area, 2-64 June 1978 2-20 Water Quality Parameters Measured in Vicinity of Bailly 2-65 Study Area

}

2-21 Water Quality Values Defined by Indiana Stream Pollution 2-66 Board, U.S. Public Health Service, or Federal EPA and Applicable to Lake Michigan in Bailly Study Area 2-22 General Water Quality Parameters, Lake Michigan and Nearshore 2 Ponds, Bailly Study Area, June 1978 2-23 Levels of Aquatic Nutrients in Lake Michigan and Nearshore 2-73 Pond Samples, Bailly F Ndy Area. June 1978 2-24 Indicators of Industrial and Organic Contamination, Nearshore 2-74 Ponds, Bailly Study Area, June 1978 2-25 Trace Elements, Nearshore Ponds, Bailly Study Area, June 1978 2-77 2-26 General Water Quality Parameters, Lake Michigan and Nearshore 2-79 Ponds, bailly Study Area, August 1978 2-27 Levels of Aquatic Nutrients, Lake Michigan and Nearshore 2-85 Ponds, Bailly Study Area, August 1978 s

x solence servlees division

TABLE OF CONTENTS (Contd)

. TABLES (Contd)

Table Title Page 2-28 Indicators of Industrial and Organic Contamination, 2-88 Nearshore Ponds, Bailly Study Area, August 1978 2-29 Trace Elements, Nearshore Ponds, Bailly Study Area, 2-90 August 1978 2-30 Trace Elements in Sediment Samples from Nearshore Ponds, 2-92 Bailly Study Acea, August 1978 ILLUSTRATIONS i

Figure Description Page 1-1 Terrestrial Sampling Locations in the Vicinity of the 1-2 Bailly Study Area 1-2 Road Route Used for Rabbit, Pheasant, and Dove Sightings 1-24 2-1 Map of Aquatic Sampling Stations in Vicinity of Bailly 2-1 Study Area Showing Stations Sampled for Various Parameters 2-2 Sediment Grain Size Distribution, Lake Michigan and 2-53 Interdunal Ponds, Bailly Study Area, 1974-1978 2-3 Some Common Macrophytes Found in Pond Areas in Vicinity of 2-55 Bailly Study Area l

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= . .

O TERRESTRIAL

SUMMARY

The summer sampling program was accomplished on schedule during July 1978. No sampling was scheduled for August or September'1978. Sampling during July in-

. cluded analysis of foliar effects, vegetation analysis, mammal observations, roadside surveys (mammals and birds), reptile and amphibian surveys, inverte-brate surveys, and soil sample analysis.

Vegetation sampling results were consistent with past years. Revegetation in the beachgrass community continued to improve in the area which was burned in i 1976. Other sampling locations showed little change other than normal plant succession.

Foliar conditions of white pine appeared to be improving, and the.large cotton-wood that appeared stressed during 1977 seemed healthy in July 1978. Herbicide application to vegetacion along the transmission right-of-way caused the destruc-tion of numerous broad-leafed plants and severely restricting the growth of black-berry plants. Additionally, woody species were cut and allowed to remain in the transmission right-of-way, and also along the southern boundary of the maple forest.

I Soil conductivity levels were well below potential stress levels, although the

., foredune samples had higher conductivity levels in May than in previous years.

l Twelve mammal species were reported during July 1978 sampling. Of all areas, Cowles Bog (wet) appeared to be utilized by the greatest number of species (9).

White-tailed deer tracks were observed in all sampling locations. Mourning Dove observations were up slightly from July 1977 results. Low water levels during summer and fall 1977 are thought to have yielded low anuran reproductive activ-ity, which in turn yicided low 1978 population densities. Only six species of herpetiles were reported although none were abundant. The number of insect fam-ilies observed was also greater than in 1977, presumably because of the warmer weather; results were comparable to 1975 and 1976 results. Five new insect fam-111es were observed during 1978.

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2 .

AQUATIC

SUMMARY

Summer 1978 aquatic sampling was accomplished in August. Samples collected

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included phytoplankton, periphyton, zooplankton, benthos, bottom sediment particle size, fisheries, and water quality. Data included in this report are those for which analyses could all be completed in the 4 weeks following sample collection, plus data from the spring 1978 quarter which had not been included in a prior report.

Lake Michigan phytoplankton samples from June 1978 revealed densities and bio-volumes comparable to tho of previous June months. There was no discernible pattern in density either from east to west or from onshore to offshore. Blue-green algae comprised 55 percent of the density but only 11 percent of the bio-volume, while diatoms contributed 77 percent of the biovolume. Dominant taxa were as in previous years and diversity was high, indications of continued stability from yecr to year.

Pond sampics from June 1978 indicated that the ponds (B, C, and Cowles Cog) all had distinct assemblages, as in the past years. Taxa which had been dominant within the three pond areas in previous years continued to be among the dominant }

forms.

No alternations of the system (changes in dentoity, biovolume, dominant taxa, diversity, chlorophyll a_, or productivity) as a result of plant construction could be detected within the June 1978 pond or lake data.

Periphyton data from June 1978 for Lake Michigan showed a similar pattern to that of phytoplankton in terms of density; small blue-green algal cells contribursd greater than 98 percent of the total density; unlike phytoplankton, they also contributed 83 percent of the biovolume for Lake Michigan samples. In the ponds, bluegreens contributed 7.5 percent of the density and less than 1 percent of the biovolume. The biovolume in Lake Michigan was dominated (56.3 percent) by the blue-green alga Oscillatoria sp., although a total of 22 taxa in Lake Michigan were observed. Diversity was lowest at Lake Michigan Station it, which is lo-cated on the northeast corner of the Bethlehem Steel Breakwater and on the fringes of the observed extent of the Bailly discharge plume. It is not thought. that plant operation or construction caused the low density and diversity, since stations closer to the discharge yielded far greater density and greater numbers of taxa. ,

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xiv solence servloes selvision

O Zooplankton samples were collected at 10 Lake Michigan and 5 nearshore pond stations. Data from June showed a mean Lake Michigan density of 7,616 organ-isms / cubic meter and a mean pond density of 258,937 organisms / cubic meter. A total of 25 taxa was collected in Lake Michigan and 40 in the nearshore ponds.

Lake Michigan samples were dominated by Bosminidae (22.0 percent), calanoid copepodids (19.6 percent) and cyclopoid copepodids (42.0 percent), while pond samples were dominated by Bosminidae (42.2 percent), Ceriodaphnia spp. (20.6 percent), and cyclopoid copepodids (12.0 percent). These same taxa were domi-nant in June 1977.

Analysis of June 1978 pond zooplankton data revealed the same number of taxa as in June 1977 (40 in both years) but 24 percent lower density in 1978 (but a dra-matic increase in Cowles Bog), although dominant taxa were the same in both years.

The decreased density is thought to represent natural variability. No unusual taxa shif ts or unexpected taxa were noted, and the ponds appear to be stable relative to their zooplankton populations. No effects of plant construction were noted.

Aquatic macrophyte composition was sLmilar to that of previous years. Some of the less common forms were missed because of the quantitative sampling techniques employed. However, sampling yielded the same dominant and/or common species as in previous years, including bullhead lily, smartweed, bladderwort, pondweed, and watermilfoil. As in previous sampling periods, Cowles Bog was characterized by a predominance of emergent species.

June 1978 benthic samples from Lake Michigan yielded 27 taxa and a mean density of 967 organisms / square meter, while the nearshore pond samples yielded 31 taxa and a mean density of 3,354 organisms / square meter. Dominant taxa in Lake Michigan samples included Tubificidae, Chironomus, Cryptochironomus, Pontoporeia affinis, and Harnischia. Dominant taxa in the ponds varied by pond but included Tubificidae, Naididae, Pisidium, Sphaerium, Chironomus, and Tanytarsus. Com-parisons of density with those of previous years showed highly variable June densities in all ponds (from 1,433 to 4,500 organisms / square meter in June 1978, indicative of the wide natural variability in population density. All of the parameters examined in 1978 (density, diversity, dominant taxa) show no obvious effects of plant construction. Benthic sediment particle size data was almost exactly like that of 1977.

1 xy solence services division l i

l

During the August fisheries sampling, a total of 225 fish (lake trout, brown trout, steelhead trout, Chinook salmon, yellow perch, rainbow smelt, gizzard shad, channel catfish, black bullhead, and unidentified minnows) were collected.

The black bullhead were collected in the nearshore ponds, the remainder of the fish in Lake Michigan. Condition factors of all fish were within normal ranges.

Fin clip marks were noted on 19 of the 40 salmonids which were collected.

Ichthyoplankton data for June 1978 revealed predominantly alewife eggs, with an average egg density of 2.95/ cubic meter. Prolarval alewife densities average 0.0457/ cubic meter. In addition to the alewife, one prolarvae percid (perch or darter) and several cyprinids (probably carp) were also collected.

Water quality parameter levels are included for both June and August. All of the values were within acceptable ranges for protection of the indigenous population, although some values were outside of Indiana standards, including plume temperature, dissolved oxygen, chloride, pH and total dissolved solfds.

Because of the ubiquitousness of the values during this period, plant operation has been discounted as a cause for all but the terperature. Within the nearshore ponds, anomalous values were recorded at various locations. How-ever, none of the concentrations were sufficiently high to present problems to the indigenous biota. The levels within the ponds and bog will be closely monitored in the future to ensure compliance with " safe" chemical concentrations.

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O 1.0 TERRESTRIAL 1.1 STATUS Summer 1978 terrestrial sampling outlined in Table 1-1 was accomplished in July by the personnel listed in Table 1-2. Sampling locations are shown in Figure 1-1. Data are tabulated for vegetation in tables 1-3 through 1-15, soils in Table 1-16, mammals in Table 1-17, and herpetofauna in Table 1-18.

A checklist of arthropod taxa for July 1978 is provided in Table 1-19.

Table 1-1 Terrestrial Ecology Sample Status as of September 1978 July

• Sample ^"#'#5'8 Parameter Locations Completed Continuing Vegetation General survey 5-mi radius x Quantitative analysis 1-6, 8 x Qualitative analysis 9-11 x Aquatic macrophytes 4-7 x Foliar effects 1-11 x Mammals Small mammal trapping 1,3,4,6,8 #

Large mammal observations 1-11 x Roadside counts for rabbits 22-mi route x Avifauna Roadside counts for pheasants and doves 22-mi route x Transect counts 1,3-6,8 #

I Reptiles and amphibians 1-8 x Invertebrates 1-8 x Soil conductivity 1-6, 8-10 x

• No sampling scheduled for August or September.

1. No sampling scheduled during sur:vner quarter.

Table 1-2 Dates and Purposes of Terrestrial Field Trips Date Personnel Sampling Performed July 9-10 Roy Greer Large mammals, roadside count, herpetofauna July 15-20 Audrey James Arthropod studies July 23-29 Tom Manthey Vegetation, foliar effects, soils l-1 solence servlees division

o LAFE MICHIGAN

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• ASH PONDS AREA O M NIPSCO PROPERTY LINE FO U b PmD POND O' 400'800'1200'1600'2000' g* gggSL gkUD 5 GRAPHIC SCALE . s po

- IRANSECTS o SD e GENERAL LOCATION LN e

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(1) BEACHGRASS COPfiUNITY (2) 00REDUNE COMMUNITY Ng 9 3 (3) IMMATURE OAK FOREST COMMUNITY

$ (4) COWLES BOG (WOODED AREA) (A) DRY (B) WET

$ (5) COWLES B0G (OPEN AREA) 7 (6) MAPLE FOREST C0ffiUNITY

- (7) EMERGENT MACRDPHYTE COMMUNITY (10) IMMATURE CAK FOREST (INTERDUNAL) C0ttiUNITY 0 (8) TRANSMIS510N CORRIDOR (11) WETLAND MEADOW COPfiUNITY l (9) SEDGE MEADOW C0tt1 UNITY (12) INDUSTRIAL ZONE E

h Figure 1-1. Terrestrial Sampling Locations in the Vicinity of the Bailly Study Area 0

3

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@ 1.2 VECETATION Table 1-3 is a checklist of plant species observed in the Bailly Study Area during the 1978 sampling.

Table 1-3 Checklist of Plant Species Observed in the Bailly Study Area, July 1978 Sampling Locations

• Scientific Name Comon Name 01 02 03 04A 048 05 06 07 08 09 10 11 Aceracaea Meole family Acer rubrum 8ed maple X X X X X Kie7 saccharinum Silver maple Alioic'eae Carpetweed family McIlugo verticillata Carpetweed Alismaceae Water-plantain family Alisma planta 10-aquatica Water plantain 5agittaria gramina Arrowhead Anacardiaceae Cashew family Rhus copallina Winged sumac EEd dIabra Smooth sumac E M radicans Poison ivy ( X X 555 typnina Nairy sumac X

M vernia Poison sumac X X Annonaceae Custard-apple family

• Alimine trileba Pawpaw Apocyanaceae Dogbane family Apocynum androsaemifolium Dogbane Ipocynum fnedium Dogbane X X j Araceae Arum family Symolocarpus foetidus 'kunk cabbage X Asclepiadaceae Milkweed family Ascleptal incarnata Swamp milkweed Asclepias purpurascens Purpleweed Ascleptas tuberosa Sutterfly-weed X Isclepias verticillata Whorled mildweed Balsaminaceae Touch-me-not family Impatiens biflora Jewelweed I X X X 8etulaceae Birch family Alnus incana 5)eckled alder X Betula Tutea Yellow birch X BeriWTdeceae Barberry family Podnony1Tum peltatum Mayacote Boraginaceae Forget-me not fastly Lithosparmum carolinense Gmelin's puccoon I X Lithospermum croceum Hairy puccoon Cactaceae Cactus family Opuntia co-pressa Prtchly pear Campanulaceae Harebell family X

Campanula rotundifolta

~ Marebell Capr'Ifol iaceae Honeysuckle family Diervilla lonicera Ncrthern bus % honeysuckle Lonicera dioca C1fmbing honeysuckle b mbucus canadensis Elderterry Viburnum acertToT Em Maple-leaved viburnum X

Viburnum dentatum Arrowwcod Vf burnu m Iantago Nannyberry Caryopnyllaceae Pick family Arenaria 50. Sand wort Lyonis albal Evening lychnis Sliene cucubatus Bladder champion Silece nTcTTTTUa Night-flowering catchfly Celestraceae Staff-tree family Celastrus scandens Sittersweet 1 ChenopodTaTese Goosefoot family Chea todium albidum Goosefoot N apod G standleyanum X CorFeTinaceae Spiderwort family Tradescentia vir1lafana 501derwort X X X Compositae Sunflower family X X Acht11ea millefolium Yarrow X Xmborsia artemistifolta Comon ragweed E brosia psilostacaya

~ #a? weed Inteanaria so. Pussytoes 1 ster cumosus Bushy aster tr~tMisIa campestrts Wormwood Aster sinertifoi6us $ tiff aster X Aster so, Aster 0elineated un Ff9ure 1 1. X indicates occurrence of a tason within sampling plots.

SOlGROG SerVIOGS dlV0SIOR

Table 1-3 (Contd) sampling Locations

• 01 02 03 04A 048 05 06 01 08 09 10 11 Scientific Name Conmon Name Compositae(contd) bidens comosa Beggar-ticks BTacni sp. I Centaurea dubia Knapweed Centaurea facea Chrysanthemu7meucanthemum On-eye daisy X

Cirsium arvense Canada thistle Conyza canadensis Horseweed ErTq W on E Tiedelphicus Conson fleabane Erfgeron ramosus Daisy fleabane X

Erfgeron strigosus Daisy fleabane Purple boneset X Eu24torium 7 Br o Tatum X l Telfanthus divicatus Woodland sunflower I

4ellanthus giganteus Tall sunflower Helianthus micrecepnalus X Nelfanthus mailIs Helianthus EIToTaris Prairie sunflower heiracium canadense Orange hawkweed I X heiracium_ sp. Hawkweed Lactuca canadensis X Blazing star

[f atris aJs era kr' is binWi Owarf dandelion a virginica Owarf dandelion X

a sp.

iiuhnia eupatorioides False boneset X X

F5dseTkTFhirta Black-eyed susan X 5enecio sp. Ragwort folTQo altissima Tall goldenrod foTTdago caesla Blue stem ed goldenrod Solidago can.densis Canada goldenrod Narrow-leaved goldenrod X X So Ca 2 2ramin(76Tia s Y5TTai n hTipida isTTair onio nsis 55TT3i3 o sp.

Hairy gold nrod X X X X X

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55ncEs sp. Sow thistle Taranacum offfeinale Dandelion X Tragopogon pratensis Goatsbeard Veronia mIssurica Drumond's ironweed Convolvulaces. Morning-glory family Convolvulus arvensis Field bindweed ConvolvuT5i septum Hedge bindweed X X

Cuscuta gronovil Dodder Ipomoea purpurea Morning-glory Cornaceae Dogwood family Cornus alternifolia Alternate-leaved dogwood Cornus amomum $11by dogwood Cornus TTorTaa Flowering dogwood Cornus ii3T5hTrera Red-oster dogwood X X X fqssa sylvatica Black tupelo X CrscTrerae Mustard family Arabis lyrata lyre-leaved rockcress Barbarea vulgaris Winter cress CilIlTe edeniu7 Sea racket Cardamine bu1E sa Spring cress X X (Fri5. so.

Gigrismatrona11s Dame's rocket

[ji idG a etalum Peppergrass

{epidium 'virq nT um Wtid peppergrass Cyperaceae sedge family X X X X X Caren so. Sedge X

[iTe7 muntenbergia X

fares penns'vivinTea FTi i*d r. .;iTTTF Spibe rush QTrm validus Bull rush (leVnaceae 01 easter family LudwiB sphaerocarpa Loosestrife (rIlidae Heath family Arcto p gny131 uva-urst_ 8earterry C ETtherta procumbens Wintergreen X X VTcETnT7pennsy l7asic um Lowbush blueterry X X

[upl6rbiaceae Spurge family X X

Flowering spurge X X

(@upnerbiacorollata horhlt humistrata Hairy spreadin9 spurge 1-4 solence servlees divielen

Table 1-3 (Contd) ta+p?ing Locations 08 09 10 4;

$1 02 03 04A 046 05 06 07 Scientific game Coven ';4w

}

Geech family l Fagaceae 8 8 A j Ouercas alta White oak l'

h;ercJs r a Red oak ' ' 4 1 8 s

Quercus velutina Black oak Geraniaceae Gerantun family Gerantuoinacalatum Wild geranium i Granium rocertianum Herb gerantur1 Granium so. Geranium Graminese Grass famtly A1rocyron traceycaul c Slender wheatorass ' <

A"enopnila breviligulata Arerican teachnrass X f Andreposen gera,rdi r Big bluester r 1

Anttropogon scoparius Little bluestem Calama1roitic canadensis Blue-j31nt reedcrass

• Calama9restE 50 Deed crass CalamovilTiilceqifcIta Sand reedgrass Cigitaria sanguinalis Craograss Eragrostic Lectinates Purple levegrass Festaca octoM ora Fescue I t t 8 Gersia5'ryzoides Rice cutarass Leersta virginica Cat;rass LMWora cognatu m Fall wittegrass K Panicum clandestie um Corn grass Fenicum dicotteur Pante crass *

• Panicum baucnucae Panic crass X 1 t

Panic am sp. Paeic trass fenicum virgatum Praic grass Foragmites cw eunis COPon reed Poa pratensis rentacky blueqrass 1 4 .t F6a 50. Bluegrass a 4 aaTEagaceae water-milfoil fartly Preserpinaca palustris Mereald-weed Hamamelidaceae uitch-eazel fanilv

• 4 I Hamamelts virgintana wittn Mazel A tridaceae trts family I

Iris versicolor Iris Sliir@ium sp. Blue-eyed qrass Juglundaceae Butternut family Juqtans cineres Batternut Jusaceae Ause fartly Juncus militarts Bayonet ruse Juncus eP usus Rush LabTa tae Mint family N

Glecmora hederates Gill-over-t'e-grourd foTlinsonia canadensis worse talm (fcopus americanus Bugle we91 lJ copus virginicus Bugle need Menths arvensis Wild mtat

'teatha so. %t y I (

FoM rda fistufosa '.lild terga mt knarda punctata Horse mint Neptes cataria Cat nio Procel1a7sTiaris Se1f-neal i

@ W 4 ;m vir7infanum Mountain rint Scutellaria galer'culata Comon Ogilter

$tacnn nati g dedge-cettle X

Machys nyssocifolia Medse-nettle

}taceys palastris Hed ;e-ne ttle 5 tB Smcoth hedqe eettle Te Er'ys-teW M a tum Onadense Gereander Lauraceae Laurel famils r u x Lindera tenrotn Spice tusn 1 X X

[ Sassafras 1 1 X M a Ga IaTEiium l

Leguminosae Letre f amily l

Aoios tuterosa Ground cut Lathyrus gab itris Vat:Miing Lapinus rereanis Lucine dedicago lupulina 91ack medic X

8 2iaia oicudotUcia 31acs locast x di u~esiriir ni m . ,4aium r t aaa r. oat s rue v Triffe,n}tHur: Alsi6e cicver Etaso. Vetcn l

i

• • 3**** **"'** * * *88*

1-5

@ Table 1-3 (Contd)

Sampling Locations

• OtA 048 05 06 07 08 09 10 11 Corunon Name C1 02 03

$cientific Name Lemnaceae Duckweed fantly Lem a minor Duckweed 1 Lentibulariaceae Bladderwort family Utricularia purpurea Purple bladderwort Liliaceae Lily family Allium canadense Wild garlic fonvaTlaria majalts Lily-of-the-valley Lilium superbum Turk's cap tily Mianthemum canadense Wild lily-of-the-valley X Polygonatum biflorum solomon's seal X

5milacina racemosa False Sclopon's seal X X Smil ' 7 H eTTata Starry false Solomon's seal X X X X EnITa. nerbacea Catbrier X X X 5mTTiT rotundtroite pound-leaf catbrier f'rTTTTum r recurvatum Prairie trillium Uvularia grandiflora large flowered bellwort Lycopodfaceae Groundoine family Lycopodium c obscurum Groundelee Lythraceae Loosestrife family Decodon verticfilatus Swamp loosestrife Najadaceae Pondweed family Nafas sp. Nalad Fotamoqenton pulcher Pondweed Potamogeton vaseyi Pondweed X Fotamogeton sp, Pondweed Nymonaceae Water lily family Brisenta schrebert Water shield Neluet>o f utes American lotus Nupnar varie2atum Bullhead lily RympheaTdirali- Fragrant waterlity Onagraceae trening primrose family Circaea alpina Enchanter's rightshade I fpTTET>Tum so. Fireweed Ludwisia schaerocarpa False loosestrife Sinothera muricata Northern evening primrose Royal fern family X

/

)

Osmundaceae Osmunda cinnamonea Cinnamon fern i Osmunda regalis Royal fere Onalidaceae Wood-sorrel family Oxalis Stricta Wood sorrel Phytolaccaceae Pokeweed family Phytolates americana Pokeweed Pinaceae Pine family Laris laricina American larch FI G bankslana Jack phe X X X PI M strobus White pine Polemoniaceae Phlos femtly Phlon bifida Phlon divaricata Blue ph10s ,

Phlos sp. Phlon i Polygalaceae Milkwort family Polygala sanquinea Purple milkwort Polygonaceae Buckwheat family 4

amphibtum bater smartweed 10 5 arifolium Tear-thumb I yaonum cocciaeum Swamp smartweed P_oly p m sagittatum Arrow-leaved tear-tnumb X X PoTyqcnum so. Smartweed

% men acetosella Sheep sorrel IFumei crisp g Curly dock pot o5<TtaTese Polypody femtly Cystopteris frogs 11s Bladder fern X X Deanstaedtle punctilobula Hay-scented fern 6cocles seastblis Sensittve fern X X 5'smunaa(Tnnanomes Cinnamon fern X PteWd7um a3uiTFum 3racten fern X X X U eTyp eits palustris Marsh fern i

j 1-6 ** I'*** "b** **8*"

Table 1-3 (Contd) 4 pling Locations

• Scientific Name Corson Name at 02 n1 04A NG 0% 06 0; na 99 in 11 Pontedertaceae Pickerel-weed family Pontederia cordata Pickerel-weed Primulaceae ~~ Primrose family Lysimachta ciliata Fringed foosestrife bstmacniaterrestris loosestrife trT M iTTi borealis Starflower Pyrolaceae Nyssa Q1 vatica 1 Ranunculaceae Crowfoot family Anemone reparia Thistleweed Anemone Mana ense Canada anemone X Aouilegia canadensis Colutine Caltha palustris w arsh marigold Ianunculus acortivus Kidney leaf buttercuo Ranunculus flabel M is Yellow mater buttercup AanuncuT E 07nnsylvanicus '

Suttercup fanuncul65 sceleratus Cursed buttercup Thalictrum polygonum Rue Rosaceae Rose family Agrimonta gryposepala Agrimony Amelanchier Canadensis Serviceberry Amelanchier Iaevis Serviceberry Aronta aroutifolia Red chokecnerry Cartaegus crus-9allt Newcastle teornacole X Fragaria virginiana Wild strawterry X Geum canadense White evens X X E virginianum Avens P5Ientilla canadense Dwarf cinquefoil Potentilla recta Cinquefoit Totentilla simples Concen cinquefoil Potentilla 50 Prunus serottna Slack Cherry I X X X X Prunus virginiana Choke cherry Rosa blanda dild rose t I X X f X Iosa sp. Rose t Fu6us allegheniensis Blackberry X FuSUs fiageliaris ceweerry x Rubus sp. Brantle X 9I da alba 9 4dow-smeet X 5pires i W ntosa Steeple bush Rubiaceae 8edstraw family Cephalanthus occidentalis Buttoneush falium a ariee Eedstraw I t

$41(umtr o um Fragrant tedstraw Autaceae Rue family Pteles trifoliata "ocey tree Salicaceae willow faaily

_ojLulus deltoides P Cottonwood A PcpuTE tre*'uT5TJes cuaking ascen Salin ntqrs Black wtllow X X faTT 50. X X Santalaceae Sandalwood family Coreanffra untellata Bastard-toadflan 5arracentaceae Pitcher plant family Sacreceata purpures Pitene r cla'it Santfragaceae Saxifrage family Ribes americanum Wild black currant Sc 5 G ariaceae Snapdragon family Aur*olaria red cularia Fcaglove Aureolaria purpurea Purple screolaria AureoTirTa virainica coway false foxglove Linaria canadensis Blue toad-flan Wlampyrum lineare Cow weest G ulus alatus

~

Sharp-winged morney flew r Penstr,nn hGiutus Ceardtongue Peast*aon so. Bearctcague 5cutellerta a-nierteufata Skull cao Terrascum tja g  !'ull t en i seconica ameritana Pernyroyal SoTanaceae Tomats family Solanum carolinense worse nettle X X X folneum dulcs' ara Nightshade X i

1-7 solence sentoes Melon

_ - . , - , - _ - - - - - - , - , -o- - - ,- -

Table 1-3 (Contd)

Sampling locations' -

03 04A 048 05 06 07 08 09 10 11 Cospon Name 01 02

$ctentific Name flitaceae Linden family A itita americana Basswood Typ W ae Cattall fastly i X T g latifolia Cattati i Ulmaceae I Ulmus rubra $11ppery elm UmEiellifWae Parsley family Cicuta bulbtfera Waterhemic(k PEsITriaca sattva Wild parsnip X X X Osmrhtfa Cla tont $weet Cicely 1

$anicula tr o TiTa Ifria aures Golden alenander mettle fastly orETeieae Boehmerta cylledrica False nettle Pilea pumila Clearweed A X X W Itis 7T57i stinging nettle I Urtica FeTs' Small stinging nettle

1. X ifrITia sp. Nettle Verbenaceae Vervatn family i

verbena hastata Blue vervatn vt6Taceae violet fastly Viola pedata Bird's foot violet x x

! VT5Ta sp, ~ vtalet

! Vitaceae Grape family X X X X Parthenocistos quinquefolia virginia creeper R X A

i Vitts sp, Grape  !

s l

l F

l l

l l

I f

(

i s

l I

• • I"**""** * "

l-8 t

t

1.2.1 QUANTITATI'!E ANALYSES 4

1.2.1.1 Beachgrass Community. As with previous samples (July 1974-1977),

American beachgrasa (Ammophila breviligulata) was the only species observed within sample plots (Table 1-4). The density of this species doubled from the 1977 sampling. The greatest increases occurred in the burned plots (TI 1976) and in the plots which were located in a swale area where additional available moisture may have favored increased growth. The trend toward increased density and dominance of beachgrass seems to indicate at least a short term stabiliza-tion of the beach sands in this community.

Table 1-4 Density, Dominance, Frequency, and Importance Values for Vegetation Sampled in Beachgrass Community, Location 1, Bailly Study Area, July 1978 Total Total Relative kelative Pelative' Importance

• Observations nominance nensity* thnette Dominance

• Doa:Inance Frecruency frequency value Asunophila breviligulata 1.870 442 756.789 100 19.247 too 10 100 300 Total 756.789

• Density is expressed as number of individuals per acre, dominance as areal coverase in square feet per acre and frequency as percent of sample plots in watch a species occurred. Importance value is the sum of the three relative values.

1.2.1.2 Foredune Community. Based on importance values, this community remained similar to the 1977 sampling. However, slight shifts in the rank of several of the important species (importance value greater than 20) has occurred.

Little bluestem (Andropogon scoparius), sand reedgrass (Calamovilfa longifolia),

goldenrod (Solidago sp.), and bittersweet (Celastrus scandens) remained the most important herbaceous class species (Table 1-5). In the shrub class, the impor-tance of basswood (Tilia americana) increased from the 1977 sampling to the level recorded during the 1978 sampling (TI 1977). The change was attributed to a relatively faster rate of growth (measured by dominance) for basswood com-pared to black oak (Quercus velutina) in the plots. The status of trees was similar to those in previous years with basswood remaining most important Be-tween July 1977 and July 1978 little stand growsh was recognized with a total

, tree basal area increase of 0.2 square feet per acre.

1-9 **3*"** **"I*** 880'8 888"

Table 1-5 Density, Dominance, Frequency, and Importance Values for Vegetation Sampled in the Foredune Community, Location 2, Bailly Study Area, July 1978 Relative Relative Relative Importance Density

• Density Dominance

• Dominance Frequency Frequency Value Scientific Name i

Herts 4.8 5.5-Amor.hlla treviligulata 3,238 0.5 44 0.2 30 Andrcocoon scgs g_Tgi 475,118 74.4 6,706 36.9 100 16.1 127.4 Mclepias Lderata 405 0.1 44 0.2 10 1.6 1.9 CAlas!nyllfA longifallA 47,755 7.5 1,263 7.0 70 11.3 25.8 CelA11tui igandens 3,642 0.6 3,179 17.5 30 4.8 22.9 2,024 0.3 348 1.9 20 3.2 5.4 CQEA1111e 1 10 1.6 1.7 Dicot I 405 0.1 tr tr 2,428 0.4 tr tr 20 3.2 3.6 DrAhA sp. 6.7 Euphort1A corallA1A 3,642 0.6 523 2.9 20 3.2 Hamamelts virginiana 405 0.1 131 0.7 10 1.6 2.4 iuhnia sp. 405 0.1 87 0.5 10 1.6 2.2 D ih5Tpermum carolinense 31.162 4.9 523 2.9 40 6.5 14.3 3,238 0.5 87 0.5 10 1.6 2.6 Panicum huachucae 3.6 ParthenocIssus quinquefolia 405 0.1 348 1.9 10 1.6 405 0.1 44 0.2 10 1.6 1.9 Quercus veTuiTna 8.1 19.6 g

IFhus radicans 16,593 2.6 1,611 8.9 50 P

e R5ii blanda 3.238 0.5 174 1.0 10 1.6 3.1 O Rud5ecT Ta7 trta 1,214 0.2 87 0.5 10 1.6 2.3 Salta sp. 2,024 0.3 218 1.2 to 1.6 3.1 SsTTTcina racemosa 3,238 0.5 87 0.5 10 1.6 2.6 Solidago griaTnTfslia 3,642 0.6 1,176 6.5 10 1.6 8.7 55TT3a3o sp. 30,353 4.7 1,002 5.5 80 12.9 23.1 Tradescantia virginiana 405 0.1 87 0.5 10 1.6 2.2 Verbascum % psus 809 0.1 87 0.5 20 3.2 3.8 ifi t t s sp. 2,833 0.4 305 1.7 10 1.6 3.7 Total 639,026 i

Shrubs 121 60 35 11.5 10 50.0 121.5 g Quercus velutina 40 270 88.5 10 50.0 178.5 g, TITTi imericana _81 Total 202 1, s

. 20 1.5 21.1 20 33.3 14.4 Pinus bankstana 8 PopuTui deTIETdes 4 10 1.9 26.8 10 16.7 53.5 l 4 10 0.6 8.5 10 16.7 35.2 g Quercus vilutina 33.~ 137.0 filia amiReana 24 60 3.1 43.7 20 g 40

. Total ,

.e l

&

• Density expressed as nunber of individuals per acre, dominance as areal coverage in square feet per acre, and

{ frequency as percent of sample plots in which a species occurred. Importance value is the sum of the three a relative values.

E tr = trace

-3

]

N._ V i

1.2.1.3 Immature Oak Forest Community. Significant importance vali e changes have occurred between the 1977 and 1978 samplings for several of the predominant herbaceous class species (Table 1-6). A decrease in importance of sedge (Carex sp.) reflected significant increases in the dominance of blue-grass (Poa sp.) and bracken fern (Pteridium aquilinum). During the monitoring period (1974-1978), bluegrass has shown a general increase in dominance, while the dominance of bracken fern has fluctuated from year to year (TI 1975-1977).

An individual red maple (Acer rubrum) seedling was observed for the first time in this type. The status of the species in the shrub stratum remained similar to the previous sampling period. Witch hazel remained the most importanc of the shrub species. Four black oaks reached tree class size between Jul3 1977 and July 1978. This contributed to the total basal area increase of 2.5 square feet per acre.

1.2.1.4 Cowles Bog (Wooded-Dry). Pennsylvania sedge (Carex pennsylvanica) and lowbush blueberry (Vaccinium pennsylvanicum) continued to dominate the herb class in this sampling location (Table 1-7). The status of these species re-mained unchanged. Together they accounted for o/ percent of the density and 72 percent of the cover (dominance). Catbriar (Smilax hebacea), which was not pre-sent in the 1977 sampling, was third in importance followed by starry false Solomon's seal (Smilicina stellata) and black cherry (Prunus serotina). An in-crease of dominance (cover) in the shrub class was reflected by the addition of five red maple, three black cherry, and one saasafras, and the loss of one black oak. In the tree stratum, one black cherry and two white oak reached tree class size, while one black oak was lost through normal tree fall.

1.2.1.5 Cowles Bog (Wooded-Wet). Rice cutgrass (Leersia oryzoides),

small stinging nettle (Urtica urens), jewelweed, and skunk cabbage (Symplocarpus foetidus) were the four most important terrestrial herb class species (Table 1-8).

Small stinging nettle was previously recorded (TI 1977) but not positively iden-tified until the 1978 sampling. Duckweed (Lemna minor) increased in importance from the 1977 sampling, probably due to near ncrmal water level in the sampling  ;

area. Shrubs remained about the same as previous samplings with red ozier dog-wood (Cornus stolonifera,) and spicebush (Lindera benzof n) being the more impor-tant species. The introduction of poison ivy (Rhus radicans) and the increase 1-11 solence servloes division

y - - - - - - - - - - - - - - - - _ , __ _ - _ _ _ _ _ _ _

Table 1-6 Density, Dominance, Frequency, and Importance Values for Vegetation Sampled in the Immature Oak Forest Community, Location 3, Bailly Study Area, July 1978 Relative Relative Relative Importance*

Scientific Name Density Density Dominance

• Dominance Frequency

• Freow ncy Value Herbs Acer rubrum 2,428 0.7 44 0.4 20 2.9 4.0

@ophta" - - 827 7.3 10 1.5 -

Carex pennsylvanica 183,329 52.7 1,132 10.0 70 10.3 73.0 Chenopodium standleyanum 2,024 0.6 tr tr 10 1.5 2.1 Cornus fiorida 405 0.1 44 0.4 10 1.5 2.0 bTiofil 405 0.1 44 0.4 10 1.5 2.0 Draba sp. 2,024 0.6 tr tr 20 2.9 3.5 Ephorbia coro11ata 3,642 1.0 87 0.8 20 2.9 4.7 Craminae I 17,402 5.0 131 1.2 10 1.5 7.7 4,047 1.2 305 2.7 50 7.4 11.3 HamameTTs virginiana fielTaifhus iiiTerocephalus 405 0.1 44 0.4 10 1.5 2.0 405 0.1 44 0.4 10 1.5 2.0 Eril la sp.

Monarda fistulosa 1,214 0.3 tr tr 10 1.5 1.8 PinTcum hauchucae 4,452 1.3 44 0.4 30 4.4 6.1 Poa sp. 75,274 21.7 348 3.1 10 1.5 26.3 Pieridium aguilinum 6,475 1.9 4,834 42.8 50 7.4 52.1 g

Rhus radicans 8,903 2.6 1,089 9.7 50 7.4 19.7 H R5ia 61anda 6,475 1.9 174 1.5 20 2.9 6.3

" 5,261 1.5 1,045 9.3 50 7.4 18.2 53iiafras albidum 4.3 5mifix Eerbacea 809 0.2 1 31 1.2 20 2.9 5mlTTcina racemosa 6,880 2.0 392 3.5 70 10.3 15.8 561tdago sp. 7,285 2.1 261 2.3 60 8.8 13.2 Taramacra officinale 405 0.1 44 0.4 10 1.5 2.0 TradescantIa virgiETana 3.238 0.9 87 0.8 30 4.4 6.1 ViccTilum penRiyTvanicum 2,024 0.6 1 31 1.2 10 1.5 3.3 Viola sp. 2,428 0.7 tr tr 10 1.5 2.2 Total 347,639 Shrubs

1. Hamamelis virginiana 1,376 65.4 853 55.1 60 40.0 160.5 13.5 96 6.2 30 20.0 39.7

$ Quercus veTirtTna 5assafrai~il5 fem 283 445 21.2 600 38.8 60 40.0 100.0 8

3 Total 2,104 0

S e Trees 12.2 4 2.3 0.3 0.8 10 9.1 S Quercus alba 90.9 287.8 Quercus GTutina 168 97.7 38.2 99.2 100

]

I Total 172 6

8

• Density expressed as number of individuals per acre, dominance as areal coverage in square feet per acre, and I frequency as percent of sample plots in which a species occurred. Importance value is the sum of the three i relative values.

• • Calculations for dominance, relative dominance, frequency, and relative frequency only.

3 tr = trace

\ y h

Table 1-7

• Density, Dominance, Frequency, and Importance Values for Vegetation Sampled 'n Cowles Bog (Wooded-Dry) Conununity, Location 4A, Bailly Study Area, July 1978 Relative Relative Relative Importance Scientific Name Density

• Density Dominance

• Dominance Frequency

• Frequency Vc.lue Herbs Acer rubrum 578 0.1 497 2.7 14 2.5 5.3 Carex pennsylvanica 298,898 70.4 2,796 15.4 57 10.3 96.1 Dicotolydonae sp. 578 0.1 tr tr 14 2.5 2.6 Fraxinus sp. 578 0.1 tr tr 14 2.5 2.6 Hamamelfs virginiana 1,734 0.4 tr tr 14 2.5 2.9 Lithospermum carolinense 3.469 0.8 tr tr 14 2.5 3.3 Poa sp. 8,672 2.0 tr tr 43 7.7 9.7 Prunus serotina 10.985 2.6 1.119 6.2 43 7.7 16.5 Quercus velutina 1,734 0.4 311 1.7 43 7.7 9.8 Roia bTanda 5.781 1.4 249 1.4 43 7.7 10.5 Rubus aTTigheniensis 578 0.1 124 0.7 14 2.5 3.3 Sassafras albidum 578 0.1 - 62 0.3 14 2.5 2.9

%Ilax herbacea 2,891 0.7 2,051 11.3 43 7.7 19.7 g ishTTcina stellata 11,875 3.3 621 3.4 57 10.3 17.0 e 5~oliaas3 o sp. 156 0.3 tr tr 14 2.5 2.8

[ iniana 1.156 0.3 tr tr 14 2.5 2.8 Tiphrosta Vaccinium penn @ syTwailcum 71,411 16.8 10,315 56.8 100 18.0 91.6 Total 424,352 Shrubs Acer rubrum 30 30.0 96 23.9 57 32.2 86.1 Prunus serotina 463 40.0 196 48.9 57 32.2 121.1 QuercusTeTstTria 289 25.0 100 24.9 43 24.3 74.2 Sassafras albidma 58 5.0 9 2.2 20 11.3 18.5 Total 1,157 0

0 Tr es

{ Lindera benzoin 6 3.0 0.4 0.4 14 8.9 12.3 3 Prunus serotina 17 8.4 2.6 2.6 14 8.9 19.9 0 Quercus alba 23 11.4 2.1 2.1 29 18.5 32.0 8 Quercus W1utina 156, 77.2 94.4 94.9 100 63.7 235.8 0

Total 202 0

• Density expressed as number of individuals per acre, dominance as areal coverage in square feet per acre, and 8 frequency as percent of sample plots in which a species occurred. Importance value is the sum of the three relative values.

y tr = trace 0

3.

O Table 1-8 Density, Dominance, Frequency, and Importance Values for Vegetation Sampled in Cowles Bog (Wooded-Wet) Community, Location 4B, Bailly Study Area, July 1978 Relative R=lative Relative 37ortance*

Dominance Frequency

• Frequency Value Scientific Name Density

• Density Dominance

• Herts 1.2 14 2.3 3.6 578 0.1 J73 Betula lutea 808 2.7 29 4.7 16.6 Caren sp. 60,127 9.2 9.3 12.4 4,047 0.6 746 2.5 57 loinus stolonifera 62 0. 2 14 2.3 3.2

~

f~yitopterTs TrililTis 4.625 0.7 2.3 2.6 578 0.1 62 0.2 14 CITT~um apartne 124 0.4 14 2.3 3.9 Ce 7umenadense 1,516 1.2 14.3 29.4 55,501 8.5 1,988 6.6 88 Tapeelens 51TTora 4.5 29 4.7 13.7 FabTiile 29,485 4.5 1.167 4.7 30.2 104,643 16.0 2,858 9. 5 29

[ W sfa~oryzoides 4.7 14 2.3 38.9 fesna minor 208,130 31.9 1.429 11.7 42,204' 6.5 870 2.9 14 2.3 haTant kaman canadense 7.8 14 2.3 16.0 Onoclea senslETls 38,735 5.9 2.361 4.7 18.5 22,547 3.5 3,101 10.3 29 OGu~ndI Unnesomea tr 29 4.7 5.3 Panicum sp. 4,047 0. 6 tr 3.6 0.1 373 1.2 14 2.3 PirtfiEocissus quinquefolia 578 3.3 14 2.3 7.6 Polygonum aHTolTum 13.297 2.0 994 2.3 2.6 p

578 0.1 62 0.2 14 e khus vern6m 14 2.3 2.4 W kit's nigra 578 0.1 tr tr 2.6

1. 578 0.1 62 0.2 14 2.3 55Tanuid5Tcamara 13.0 57 9.3 24.8 16,187 2.5 3,91 5 5 -

3TEarFoet bimus rubra tdus 2,313 0.4 62 0. 2 14 2.3 2.9 5,203 0.8 373 1.2 43 7.0 9.0 DFITca 4 tea 25.4 14 2.3 31.1 (TriTia urens 21 % 9 3.4 7.64 3 5.0 6,938 1.1 497 1.6 14 2.3 (silii sp. 2.3 2.6 I 734 0.3 tr tr 14 frio T sp.

~1o141 652 III Shrubs 5.1 653 11.3 43 17.6 34.0 Acer rubrum 176 17.3 58 2.5 174 3.0 29 11.8 Aln7s Tncina 32.3 57 23.3 94.8 Cornus stilonifera 867 38.5 1.871 752 33.4 2,306 39.8 29 11.8 85.0

[Tniera~5enzoin ,

3. 0 29 11.8 17.3

1. khus radicans 58 2. 5 174 10.3 479 8.3 29 11.8 30.4

$. kh7u veinlF 231 131 2.3 29 11.8 21.8

_ 58 7.7 9 KITT= n' ara foiaT' 2.140 3

0 0 Trees 55.6 29.0 $8.9 86 43.2 157.7 110 Acer rubrum ~

35 17.7 5.0 10.2 43 ' 21.6 49.5 '

IIeisli~lu~ tee 7.0 15.3 ssa sylvatica 12 6.1 1.1 2.2 14

~

3. 0 1.1 2.2 14 7. 0 12.2

• unus serollni 6

7. 0 21.4 0 6 3.0 5.6 11.4 14 IITTrnl_ir_a 7.0 24.7 0 6.1 23 11.6 3.0 14 5assafras albidum 7.0 18.9 O Ulmus ruEra 6 3.0 4.4 8.9 14

& loial~ i98

• Density empressed as number of individuals per acre, dominance as areal coverage in square feet per acre, O and frequency as percent of sample plots in which a species occurred. Importance value is the sum of k the three relative values. t 3

tr

• trace g)

in density of red ozier dogwood, spicebush, and poison sumac (Rhus vernix) accounted for much of the increase in dominance.

The status of tree class species remained similar to previous sampling years, except for the gain of one slippery elm (Ulmus rubra) and one red maple, and the loss of four black willow (Salix nigra). The loss of the black willow oc-curred in a single plot. Two of the four trees were leaning during the 1977 sampling (TI 1977) and the other two had fallen since that period.

Water fluctuations in the sampling area appeared to have contributed to the windfall of the willows.

1.2.1.6 Cowles Bog (0 pen). Rice cutgrass and jewelweed were the most important herb class species with almost identical importance values (61.2 and 60.1, respectively, Table 1-9). These were fellowed by cattail, arrow-leaved tearthumb (Polygonum sagittatum), anc clearweed (Pilea pumila). Hedge-nettle (Stachys palustris) increased significantly in importance, primarily because of an increase in its dominance. No shrubs or trees occurred in the sampling plots.

1.2.1.7 Maple Forest Community. Black cherry remained the most impor-tant herb class species followed by jewelweed, virginiana creeper (Parthenocissus quinquefolia), and spicebush (Table 1-10). Black cherry replaced red maple as the most important species, and one flowering dogwood was observed for the first time in the shrub class plots. The increase in importance of black cherry reflected an increase of three individuals for this species and a loss of one red maple.

The status of the tree species remained the same as during the 1977 sampling with red maple as the dominant species. Between 1977 and 1978 sampling, two red maples and one black locust (Robinia pseudoacacia) were lost through normal treefall.

1.2.1.8 Emergent Macrophyte Community. Bullhead lily 'Nuphar variegatum) remained the most important species (Table 1-11). Only one species of pondweed was observed and it increased in importance over the 1977 sampling. In addition common shoreline emergent species appeared to be similar to the previous years.

1-15 sc3ence servlees division

Table 1-9 Density, Dominance, Frequency, and Importance Values for Vegetation Sampled in Cowles Bog (0 pen) Connunity, Location 5, Bailly Study Area, July 1978 Relative Relative Importance*

Relative Frequency Value Density

• Density Dominance

• Dominance frequency

• Scientific Nane tierbs 20 3.6 3.8 Bidens sp. 2,024 0.1 tr tr 4.2 7,689 0.7 522 1.7 10 1.8 Convolvulus septum 40 7.1 8.0 Cuscuta gronavii 8,094 0. 8 44 0.1 tr 10 1.8 1.8 Cystopteris fragalls 405 <0.1 tr 4.2 4,452 0.4 609 2.0 10 1.8 Eupatorium purpureum 80 14.3 61.2 331,854 30.8 5,003 16.1 Incattens blir ora 15.8 70 12.5 60.1 Leerzf a oryzoides 342,376 31.8 4.916 29.2 168,760 15.7 1,4 36 4.6 50 8.9 Pilea puilla 22.9 60 10.7 37.8 P3Ty~donum sagittatum 45,326 4.2 7,091 0.9 653 2.1 10 1.8 4.8 Rosa sp. 9,308 4,452 0.4 348 1.1 10 1.8 3.3 55Tanum carolinense 40 7.1 26.6 25,901 2.4 5,307 17.1 r 5tachys palustris 4,698 15.1 100 17.9 43.0

Tgha latifolia 107,246 10.0 8.0 17,402 1.6 305 1.0 30 5.4

$ Drtica sp. 0.3 20 3.6 4.1 Zizia aurea 2.428 0.2 87 7 0tal 1,077,717

• Density expressed as number of individuals per acre, dominance as areal coverage in square feet per acre.

and frequency as percent of sample plots in which a species occurred. Importance value is the sum of j the three relative values.

tr = trace e

0 F

I.

i, O

r a

Y

Table 1-10 o Density, Dominance, Frequency, and Importance Values for Vegetation Sampled in Maple Forest Community, Location 6, Bailly Study Area, July 1978 Relative Relative Relative Importance*

Scientific Name Density

• Density Dominance

• Dominance frequency

• Frequency Value Herbs Acer rubrum 5,666 6.1 44 0.5 50 11.9 18.5 CBre~x sp. 3,238 3.5 tr tr 10 2.4 5.9 Circaea alpina 10,522 11.3 392 4.3 10 2.4 18.0 Cornus fTE7r iG 3,238 3.5 44 0.5 30 7.1 11.1 taITum apartne 405 0.4 tr tr 10 2.4 2.8 Geum cahadenie 7,689 8.3 783 8.5 10 2.4 19.2 Olecoma hederacea 2,428 2.6 tr tr 20 4.8 7.4 impatiens bTflora 14.569 15.7 1,436 15.6 50 11.9 43.2

[Tndera benzoin 5,666 6.1 1,001 10.9 30 7.1 24.1 Parthenocissus quinquefolia 11,736 12.6 653 7.1 30 7.1 26.8 Prunus serotina 19,830 21.3 3,328 41.7 50 11.9 74.9 Quercus velutina 405 0.4 tr tr 10 2.4 2.8 Rosa ETanda 4,452 4.8 566 6.2 30 7.1 18.1

~ 5sETcula trifoliata 405 0.4 tr tr 10 2.4 2.8 I Smilax herbacea 405 0.4 tr tr 10 2.4 2.8 C EsiTIT ina racemosa 1,214 1.3 131 1.4 30 7.1 9.8 Drtica dioca 405 0.4 174 1.9 10 2.4 4.7 IIili aurea 809 0.9 131 1.4 20 4.8 7.1 T5tal 93,082 Shrubs Acer rubrum 160 21.0 2,523 29.9 60 54.5 105.4 Cornus florida 40 5.3 174 2.1 20 18.2 25.6 Prunus serotina 1(il 73.7 5,742 68.0 30 27.3 169.0 751al 761

1. Trees

{ A w N trum 206 74.1 63.3 67.1 90 50.0 191.2 3 8 2.9 1.1 1.2 10 5.6 9.7 0 U PHs M M.us ,er5Hnairas-galli 24 8.6 16.8 17.8 20 11.1 37.5 8 l)uercu's7 ba 8 2.9 1.7 1.8 10 5.6 10.3 p Robinia pseudo-acacia Sassafras albidum _a 24 8.6 2.9 9.7 1.7 10.3 1.8 40 10 22.2 5.6 41.1 10.3 16 Total 278

• Density espressed as number of individuals per acre, dominance as areal coverage in square feet per acre, 8 and frequency as percent of sample plots in which a species occurred. Importance value is the sum of

.6 the three relative values.

'i e tr = trace 4

3 4

Table 1-11 Density, Dominance, Frequency, and Importance Values for Vegetation Sampled in '

Emergent Macrophyte Community, Location 7, Bailly Study Area, July 1978 Relative Relative Relative Importance*

Scientific Name Density

• Density Dominance

• Dominance Frequency

• Frequency Value Nuphar variegatum 9,066 90.3 768 92.8 0.28 17.8 260.9 Fotamogeton sp. 971 9.7 60 7.2 0.08 22.2 39.1 Total 10.037

• Density expressed as number of bdividuals per acre, dominance as areal coverage in square feet per acre, and frequency as percent of sample plots in which a species occurred. importance value is the sum of the three relative values.

Table 1-12 Density, Dominance, Frequency, and Importance Values for Vegetation Sampled in Transmission Corridor, Location 8, Bailly Study Area, July 1978 H

l Belative Belative Bele t tve importance*

>-8 F rewenc y* Frequenc y value 00 5c teattinc Isame Dea s t ty* Deas t ty Dominence* Dustnance w

herbs Ot11ea stilefolium 2,024 0.1 131 0. 3 20 3.5 3.9 6e6.776 47.6 14,616 35.3 70 12.3 95.2 Apo2 @num medium-A7wtr7 1.214 fg4~ifrardi~~

0. 4 348 0. 8 10 1.8 2. 7 Carc}a sp. 61,514 4.3 261 0. 6 10 1.8 6. 7 Ciillum arvense 1,619 0. 3 21s 0. 5 20 1.5 4.1 l Di2Ftjleadosii 809 0.1 87 0. 2 to 1.8 2.1 Fricarifil in tena 809 0.1 44 0.1 to I8 2. 0 i41Taithui ~ 4ieus 1.214 0.1 261 0. 6 10 1.8 2.5

[

1Hi"verik or 21,449 1.5 1 ,1 11 2.7 10 1.8 6. 0

~

[aciuia lanadensis 405 0.1 44 0.1 10 1.8 1.9 (eifile irjiildef 307,977 21.3 5,046 12.2 40 7.0 40.5 i

finoihirianJIEata 405 0.1 348 0. 8 10 1.8 2. 6 enoilef sessillis~ 12.950 0.9 1,523 3.7 30 5. 3 9. 9 I

g l*iil ' * '83 *** " 53 '5 O. kthenoitis M A' sI'd d"*quefoln ta 3,642 0. 3 261 0. 6 10 8.8 2. 7 Poa ss.

~ ~ -

25,4 % 1.8 392 0.9 20 3.5 6.2 8 0.1 0.1 10 1.8 2.0 g Polygonum sagittatisa 809 7.285 0. 5 5.568 44 13.5 20 3.5 17.5 0 Picanthema virilaianum 3,915 9.5 90 15.8 29.0 Irubuf fle341 Tarts ~- 53.016 3. 7 0 Hudbedia hirta~ 3.238 0. 2 174 0. 4 20 3.5 4.1 Solidagsgram'is t folta 98,342 6.8 1,001 2. 4 20 3. 5 12.7 Solidago sp. 4,452 0.3 522 1.3 30 5. 3 6.9 e 123,414 8.5 4,350 10.5 4') 7.0 26.0 th'lygterws Tridesiiniia g@alestets

~ Tilina~ 4,856 0.3 131 0. 3 10 1.8 2.4 h IUle%ria 4.M2 0. 3 522 1.3 10 1.8 3.4 y Total- i 443.970 I

w O

• Density empressed as munter of lad 6viduals per acre. dominance as areal coverage in sware feet per acre, S and frequency as percent of sample plots la whkin a species occurred. Importance value is the sum of S the three relative values.

l I

e 0

3 i

l l

U L Q

1.2.1.9 Transmission Corridor. Several weeks prior to the July 1978 sampling, the transmission corridor was treated with a broad-leaf herbicide and some of the shrubs and trees were cut along the edge of the corridor.

The line maintenance has affected the character of the sampling plots. The herbicide inhibited growth of broad-leaf (Dicot) species but had little or no apparent adverse effects on narrow-1,eaf (Monocot) species. The selective nature of the herbicide is apparent from the significant increase in importance of big bluestem (Andropogon gerardi) and rice cutgrass (Table 1-12). In addi-tion, these species showed little or no visible stress, while most broad-leaf species had leaf necrosis. No shrubs or trees were present during the sampling.

1.2.2 QUALITATIVE ANALYSIS 1.2.2.1 Sedge Meadow Community. The common species in past samplings (1974-1977) were again present (Table 1-13). However, daisy fleabane (Erigeron strigosus), horse mint (Monarda punctata), and goat's rue (Tephrosia virginiana),

species which were observed during the July 1977 sampling, were not observed in the current sampling; Canadian anemone (Anemone canadense), not observed in pre-vious sampling, was recorded for July 1978. The species not recorded in 1978 were relatively uncommon in 1977; dif ferences in presei.:e/ absence are as-cribed to natural fluctuations in these annual plants.

Table 1-13 Vegetational Taxa Observed in Sedge Meadow Community, Location 9, Bailly Study Area, July 1978 Scientific Name Common Name Acer rubrum Red maple Anemone canadensis Canadian anemone Carex sp. Sedge GoTo~rbia corollata Flowering spurge Hamamelis virginiana Witch-hazel Hieracium sp. Hawkweed Monarda fistulosa Wild bergamot Panicum sp. Panic grass Pinus banksiana Jack pine Poa sp. Bluegrass Prunus serotina Black cherry Pteridium aquilinum Bracken fern Quercus velutina Black oak Rosa blanda Pale rose Sassafras albidum Sassafras Smilacina stellata Starry-false-Solomon's seal Tradescantia virginiana Spiderwort Vaccinium pennsylvanicum Lowbush blueberry Vitis sp. Wild grape 1-19 solence servlees division

@1.2.2.2 Immature Oak Forest (Interdunal) Community. Three taxa observed from the 1977 sampling (Table 1-14)- Lupine (Lupinus perennis), wild lily-of-the-valley (Maianthemum canadense), and beardtongue (Penstemon hirsutus) -

were not observed during July 1978; no new species were recorded for this type.

Table 1-14 Vegetational Taxa Observed in Immature Oak Forest (Interdunal) Community, Location 10, Bailly Study Area, July 1978 Scientific Name Comon Name Andropogon scoparius Little bluestem Apocynum medium Dogbane Artemisia campestris Wormwood carex sp. Sedge Erigeron strigosus Daisy fleabane Euphorbia corollata Flowering spurge Hamamelis virginiana Witch-hazel Helianthus divericatus Woodland sunflower Hieracium sp. Hawkweed Monarda fistulosa Wild bergamot Opuntia compressa Prickly pear Panicum sp.

Parthenocissus quinquefolia Panic grass Virginia creeper -

}

Pinus banksiana Jack pine Poa sp. Bluegrass P Enus serotina Black cherry Pteridium aquilinum Bracken fern Quercus velutina Black oak Rosa blanda Pale rose Rubus sp. Blackberry Sassafras albidum Sassafras Smilacina stellata Starry-false-Solomon's-seal Solidago sp. Goldenrod Tephrosia virginiana Goat's rue Tradescantia virginiana Spiderwort Vaccinium pennsylvanicum Lowbush blueberry Vitis sp. Wild grape 1.2.2.3 Wet Meadow Community. Several species not recorded for the 1977 sampling were observed during July 1978. All of the species (Table 1-15) are l common for this type and occur throughout the dunes area (Peattie 1930).

j 1-20 science servloes diviolon

Table 1-15 Vegetational Taxa Obscrved in Wetland Meadow Community, Location 11. Bailly Study Area, July 1978 Scientific Name Comon Name Andropogon gerardii Big bluestem Cephalanthus occidentalis Button bush Impatiens biflora Jewelweed Eeersia oryzoides Cutgrass Rhus vernix Poison sumac Rfius u radicans Posson ivy raTTx sp. Willow Salix'nigra Blackwillow Sambucus canadensis Elderberry Solanum dulcamara Nightshade Solanum c_golinense Horsenettle Spirea alba Meadow-sweet' Spirea tomentosa Steeple bush Typha latifolia Cattail Urtica dioca Stinging nettle l

1.2.3 FOLIAR EFFECTS. The large cottonwood which exhibited foliar damage {

in 1977 (TI 1977) appeared healthy during July 1978, and the white pines (Pinus strobus) which incurred foliar damage during 1976 (TI September 1977a), appeared to be recovering, although needle browning was still observed on old needles.

Stress was observed along the South Shore railroad and Northern Indiana Public Service Company right-of-way area (sampling location 8, Figure 1-1).

1.2.4 SOIL CONDUCTIVITY. As in 1977 (TI 1978) the mean conductivity values for all sampling locations were greater in May than in July during 1978 (Table 1-16). Generally, the May 1978 values were less than those in 1977, and the July 1978 values were slightly greater than those in 1977. The mean values for the foredune community were unusually high during May 1978 but were near normal in July 1978. However, no mean values approached the levels of conductivity that would indicate that salinity levels were detrimental to salt-sensitive species. Future monitoring will help establish patterns of variation in this community.

1-21 solence servlees divisten

Table 1-16 Mean Soil Conductivity (pmhos/cm) Values from 10 Locations

)'

/

Bailly Study Area, May, July, 1978 Sampling Location May Jul Beachgrass (Location 1) 228.6 148.5 Foredune (Location 2) 754.5 196.0 Immature Oak Forest (Location 3) 242.3 137.4 Cowles Bog (Wooded-Dry) (Location 4A) 300.5 209.6 Cowles Bog (Wooded-Wet) (Location 48) 389.6 267.1 Cowles Bog (0 pen) (Location 5) 418.0 368.6 Maple Forest (Location 6) 631.1 339.3 Transmission Corridor (Location 8) 387.4 189.4 Sedge Meadow (Location 9) 250.2 152.4 Immature Oak Forest (Interdunal) 377.3 164.9 (Location 10)

Wetland Meadow (Location 11)

• 79.3

• Sample locations submerged; valid samples could not be taken.

1.3 VERTEBRATES s.

)

1.3.1 MAMMALS. Twelve species of mammals were reported from the Bailly Study Area during July 1978 (Table 1-17). The white-tailed deer (Odocoileus virginianus) was the only mammal reported from all sampling locations.

Table 1-17 Number of Mammals Reported from 8 Sampling Locations at the Bailly Study Area, July 1978 Sampling Location Ima ture Cowles Cowles Maple Emergent Transmissio.

Corunon Name Beachgrass Foredune Oak Forest 3og(W) Bog (0) Forest Macrophyte Corridor Opossum x x x x x x x x x x Eastern mole x x x x j Eastern cottontall rabbit x Eastern chipmunk 1 10 2 Woodchuck a x x Fox squirrel 2 Red squirrel 1 Muskrat I striped skunk 1 x x x 2 x x x Raccoon Long-tailed weasel

• White-tailed deer x x x 1 x x x x

./

Road k111 just off the imediate study area l

l-22 solence servlees division I

i

o 1.3.1.1 Lakefront Communities. Four of the seven mammals reported occurred 1

in all three locales (Table 1-17). Six of the seven were present during past summers, except the striped skunk (Mephitis mephitis), which had not previously been reported from any of the three sampling locations.

1.3.1.2 Cowles Bog (Wooded). Most (9) of the mammals repcrted during July on the site occurred A this locale. The eastern chipmunk (Taains striatus) was the most commonly sighted species. Two raccoons (procyon lotor) were seen near the guard house at the eastern end of the trail that enters the wooded bog.

Raccoons are probably more common than sightings reveal, since they are nocturnal and rarely seen during daylight hours.

1.3.1.3 Cowles Bog (Open). All six species of mammals reported from this locale were noted from tracks or other signs. All of the noted species commonly frequent or inhabit the open bog area, as documented in previous sampling periods.

1.3.1.4 Maple Forest. The eastern chipmunk was the only species sighted in this locais. Four additional species were reported from observation of tracks or other visible signs.

1.3.1.5 Emergent Macrophyte. The only muskrat (Ondatra zibethica) sighting on the Bailly Study Area occurred in this locale. Muskrats were not sighted on the study area during May nor were any houses observed. The individual sighted during July was therefore presumed to be a migrant.

1.3.1.6 Transmission Corridor. Of the three species reported from the trans-mission corridor, only the eastern cottontail (Sylvilagus floridanus) was sighted.

Signs of raccoon and white-tailed deer were also detected.

1.3.1.7 Cottontail Rabbit Road Route. The seven cottontail sightings during July 1978 along the 22-mile road route (Figure 1-2) represented next to the low-est number of observations since the survey began; the fewest sightings (6) were in April 1975. Temperatures were low during all three surveys, although the low number of observations is believed to be a continuation of the las in the cotton-tail population noted in May 1978 surveys. The low numbers are noteworthy since they aid in establishment of baseline densities with which future operational (i.e.,

Bailly N-1 on-line) densities can be compared.

1-23 solence servlees devielen

Stop No. Observed 4

6 1

1

)'

10 1 15 1 17 1 18 1

/

DUN T jg LAKE MICHIGAN 13 P ARK

[ 15 12 16 22 g to

' POND C VEST ROAD EAST ROAD

_ 2 @1COWLES B0G Q C00 ll II RT 12 PORT OF INDIANA 9 f _

BETHLEHEM AND NORTH ROAD l ig

' MIDWEST STEEL 39 i BURNS DITCH 20 7

2

) 3 3 6 5

START HERE STOP HERE Figure 1-2. Road Route Used for Rabbit, Pheasant, and Dove Sightings 1.3.2 AVIFAUNA 1.3.2.1 Roadside Pheasant and Dove Count. Mourning Dove (Zenaida macroura) f sightings along the 22-mile road route (Figure 1-2) were up slightly (7 to 10) over 1977 summer counts. The survey revealed a significant increase in doves l over the spring 1978 count (2 to 10).

Stop Number l 4 1 6 1 15 2 17 1 19 1 20 2 21 2 1-24 solence servlees divleton

No Ring-necked Pheasants were seen or heard during the summer 1978 survey.

1.3.3 HERPETOFAUNA. July 1978 sampling produced six amphibian and reptile i

species (Table 1-18). None of the species reported were common. Only one spe- ,

cies, the green frog (Rana clamitans), was reported in more than one sampling location. Generally amphibians and= reptiles appeared more scarce than in prior I summers, probably due to the long colder than normal winter.

l Table 1-18 Number of Amphibians and Reptiles Reported From 8 Sampling Locations at the Bailly Study Area, July 1978 Sampling Location Ima ture Cowles Cowles Maple Emergent Transmission Comon Name Beachgrass foredune Oak Forest Bog (W) Bog (0) Forest Macrophyte Corridor ,

American toad 1 i Bull frog 3 Green frog 4 1 2 Painted turtle 7 Northern water snake 1 Eastern garter snake 1 1.3.3.1 Lakefront Communities. No herpetofauna were reported from these three sampling locations during July 1978. Herpetofauna were located in these communities in past sampling periods. The low densities would appear related to the overall low site density of herpetofauna probably caused by low water levels in the previous year.

1.3.3.2 Cowles Bog Nooded). The eastern garter snake (Thamnophis sirtalis) i and green frog were the only herpetiles reported from the wooded bog. In past sum-mers, green frogs have been fairly numerous in this locale. Low water levels dur-ing summer and fall 1977 may have been responsible for the low anuran abundance, since anurans require water for egg deposition and overwintering before emergence during the following spring and summer.

1-25 solence servlees divessen

1.3.3.3 Cowles Bog (Open). No herpetiles were observed in the open bog dur-ing July 1978. As in the lakefront communities, the absence of herpetofauna is -

unusual, but would appear related to overall low site densities of herpetofauna.

1.3.3.4 Maple Forest. One green frog was observed along the small drainage ditch that intersects the maple forest. Green frogs are uncommon in this locale.

1.3.3.5 Emergent Macrophyte. The highest number of herpetile species (4) observed on the Bailly Study Area occurred in this locale. Seven painted turtles (Chrysemys picta) were counted during the July survey. Three additional species, the bullfrog (Rana catesbeiana), green frog, and northern water snake (Natrix sipedon) were also observed in this locale.

1.3.3.6 Transmission Corridor. The American toad (Bufo americanus) was the only herpetile observed in the transmission corridor during July. Although water was more available in this and other sampling locales on the site during July 1978, amphibian and reptile numbers and species were still fairly low.

1 . ~. INVERTEBRATES 1.4.1 SAMPLE LOCATIONS AND HABITAT CONDITIONS. The scheduled complement of invertebrate samples was taken: sweepnet and litter samples from locations 1, 2, 3, 4A, 4B, 6, and 8; dipnet samples from locations 2, 4B, 5, 6, 7, and 3; and lighttrap samples from 1, 2, 3, 4B, 6, and 8 (Figure 1-1). The two aquatic locations not sampled during the previous summer, 2 and 4B, again contained suf-ficient water for sampling. The level of standing water in location 4B, the wet woods of Cowles Bog, is maintained naturally and typicially fluctuates with the season and amount of precipitation; the low level of last summer (1977) reflected the drought conditions prevalent in the area during spring 1977. The cattail /

shallow pool habitat of location 2, on the other hand, is maintained partially by drainage shunted into the location. The area was drained in 1977 to allow construction of a fence around the Bailly plant, but the viable conditions of the vegetation and advanced recovery of the arthropod community a year later indicate disturbance of the habitat was only temporary and probably did not im-pact the site's entomological fauna.

)

1-26 solence servlees division

The more normal weather conditions preceding and during 1978 entomological sam-pling were reflected not only in the complement of samples taken but also in the sampling results. With the exception of cool temperatures (approximately 58 to 63*F) during the three lighttrap periods, optimal sampling conditions prevailed and insect activity was typical of past years (other than 1977).

1.4.2 RESULTS. Entomological taxa identified during July 1978 sampling on the Bailly site are listed on Table 1-19. The number of insect families ob-served (140) was comparable to those observed in summers of 1975 and 1976 and considerably more (by two dozen) than collected in 1977. Composition and abun-dance varied somewhat from previous sampling periods, reflecting characteristic population fluctuations and emergence patterns.

Five 10 sect families were newly observed on the site. Clusters of termites (Isopterd were noted in the foredune attacking the vegetation sampling plot stakes. Teronites are categorized habitat-wise (aQ well as taxonomically) by where they are found, with most forms inhabiting dampwood, drywood, powderpost, or subterranean habitats. Those found on the site were subterranean forms that nest in soil and either tunnel to wood or construct earthen tubes to wood not in contact with soil. Subterranean forms in eastern United States are in the Rhino-termitidae, and Reticulitermes flavipes is the common species.

A second new: observed group was the giant silkworm moths (Lepidoptera:Satur-niidae) represented by the polyphemus moth (Antheraea polyphemus), a large (5-inch vingspread) yellowish brown moth with an eyespot in each hindwing and the io moth (Automeris p), a smaller (3-inch wingspread), brighter yellow-brown species, also with hindwing eyespots. The polyphemus was seen in two locations:

the immature oak community at the lighttrap and the dry woods of Cowles Bog on Icaf litter. The caterpillar of this common species, which ranges to the western-most states, feeds on several trees, including oak, hickory, elm, and birch. The io was swept from vegetation in the maple woods. The io is a common species also, i but it ranges westward only to the Great Plains. The larva, a highly spiny green l caterpillar, is a general feeder.

1 Reticulated beetles (Coleoptera:Cupeidae, velvet ants (Hymenoptera:Mutillidae),

and mydas flies (Diptera:Mydidae) were the other three newly sighted groups. l I

l l-27 solence servloes division

p..

Table 1-19 Checklist of Entomological Taxa Collected in the Bailly Study Area, July 1978

)

Sampling Stations sg 5 6 7 e

!  ! ,,3 _, sA _

Jasmeture Cowles 3o9 Cowles Bog Dunes neple Transatssion Dry moods eat Woon Crees Woods road e Coreteor tasa seecagress Foreduae ost I

Proture (protureas)

Colleseoia (sortagtatts) I I I I Poeuridae I I I I I I I

Isotent6ae I I

I Entapobeyldae Ephoserooters (mayf16es)

Caealdae I I

/

caea3 see.

saF Tdae I (10ena sp.

odonais Tiragonfites, da set files)

Aesnaies. (dregaafites) I Aes 59. I Isa a gu LilwrTTu iose (dragonfites) I I

tajemi sp. I be 1ula sg. I ytl las loritDeants I FTit ~e I

I I Weir # . tum Leitica. (esreTTTTis) I I I tesate ret t en%1ect s I CodegrionTTaiTfsieTeites) I I

taalta2ai TEanura soo so.

ortnoWre Tgressnoopers, katrelds, reacnes, etc.) I I

I Acrteldae (grassnoopers) I ptsseitetr fetirgoiTTEa ceraitas (Careitaa grassnoo,er) I I I Th4V33)

Grylltese (crtchets) I Cecaatau l 30 PhismeIIIae (weikingsticts) ma $$k!

Blattidae (reacnes)

I I

Paryotletta etretatta Decesotera (earwiss) \

)

I Fortculidae '

Isosters (terettes) I Antaoterettideo Pieceptere (stonefiles)

Periteae I Pec'es picaissiTr,e{a glectda(esicTJsl I I I PseudoceeCillleae I Psocidae Thysaanotere (thrtos)

I thrtsida.

heatsters (bugs)

Certaidae (.atertmtseal I I I il are spp. I ortse app. I r

actonoclflee Ibacksonsuiers) I I notaaette spp.

PleTimelTeid .eter nues) I I Ptes striais I seisstsitTlae (gtent ter hugs) I pelosta=a so.

Ger7dieTester strtders) I I r4rris sp. I I

%TnEate1 so. I veTTiesfThroadsnoutderee .eter striders) meseveiledee ( tee treeeers)

I p

reiu s Th..(aIsasta bugs) I Phi 5al,idae (seeuse bugs) I I

Pwea se.-~

tisgidae (lace bugs) I torvt*uca_ arcuata I F.e .oraee Lygaeldae (seed bugs)

I (fous so. I Geocorts sp. I Ttras53amus faticus I I

=GTTae Geel bugs)

I (1291 Ida_eotects (tarstsaed plaat bug) I

'#Taeonest 1 so. I MIF46tylus ruN orats t I I I I.~ tersalls PentafaeI3ae (stina bugs)

I Cosaepeat t teaculet e I I ETTeq Toers I 5ol

- vbee is.

homoitiFiTnoevers. acates, etc.)

wacidae (treeaoooers) I I I tyrtotoeu I I I Ciceua ir.le see. ose che,o.es) I I I I I Chterettttia so. -

g,iena so. I 1-28 solence servloes division

_ . . _ . . ~

o Table 1-19 (Contd)

' l.coi t., St. tie.s i a a u . i e r l w tore c ies se, co.i.s so, cons =.. i e Tr..s. .s, -1 fu. soun,r.n rue.. en ory n ut

• n tren non Pe corri.or ier.cc to a r .s w.

te,u..,r. se.

m i I a i

r.,.s.u..,i c ...

n.. i . w.

s a . w.

I.55iervs 59 E 3

vi[F '

.i.s biit riw.

s.7+T mmt u.

ti M =

a x

PE .i. se. gs)

I I A I R I -I e

cerc.opis.~.e(sett.t.ieon,4 cei <*i = ,i.. ,e,si a i i I

claii o.e cu nIcis.t14 6. t. hoppers) i  : i acani .eine .ii. t. o..i..tn nes> en) au,.. i <x.. i i a

es.n..no io ine i o.o.nl ei..o.ces  : i n ance c.

ceien.te..n.e i,a.ncoi.tn ononi 1 cocel c Cupes i lTu ii a cia.n.nn.sT,on.ger cio ns eintiet .

c. d t esl 'I a

tst.a,fos re'h. o PfertilTr us s p. s

%is -

.ett.ifa.e..ur iig .ner 6.ece)

.n I A o,tTrklu2 .e w. t,re.  :

wr iceporvs co.nens siins ... ., netie) a s a i

u. .ger~- sp.

E t I I I

e+ v5 sp.

.,n ITine c eer sc..mer noni 3 Beresug sp MT6pogev sp. ,

or byf se. I dberg ebtus.t. t 3 I ar.mg sp.

I I i rq St.~phy_n___t ee+e1 soII.Tne tr. e beeties) a e a a to.os r

., @ .s'59se. I fe therwieged beetles)

PtPtfTI.I t ell. sp. A 3

61. teetles) I Ps.i.TTolid.i c th.N e ( (s.ortwl.ge. s.l se r teeties) E 1 8 cli L.ffati.n

. ope t (c5htfic.ff tn) '

NdThieN" urhon s

ciem.2ea.e e o,.ece.

r.n se.see.ces)s ,

a E1. l.eiT3'iTcTscETut'eiT tere teus so. a n (TETbgalers t e t t ei t s  :

e.orTim7iet. luc aoren)

• ee ier a

.efh=T.t.e t.t.poic*gp enTI,iettn) '

e r a a rg' on. so. sp. I i 9(IM.

es sp p.M.burTJu (sniat.e fu.g s antles)

X X 3 Ci ern sp. I utU3'iTTI.e (s. buttn) I I

t. r I si sc..e.,er besties) t.frwa etervi so.Cve r.LO. ss.nute brou rt 3 troGTT'i.e7.pien.s

• s.lo4u . f s

,e I c.R.c tawg'.Et.tqstle Tai 1111.3 TTi.oit.v.ous beetles) i~sieetes) s a

a y n.e T 2e .

convereeg.

s a

a RPo4.*

• litw s 19 .g1qQgtr'bc **pwactat e s p re 3 4.id.r.i.d.

iTT t u.T,Tije 0Tle houer butin) li.te.v1 sp t c.;Tiai.e ( .tute int notini

r. .

TTHe f Peh.en..,t(a.iie t a Sr eiise.e (tcet tg tline fio.fl erec 6eetles) tuties)

• -o.n spo t -a ci e untini airea.Trde f .ec Is-c.uj so.

an. I a

ieren l-29 science services division

Table 1-19 (Contd)

$amotins Stations 1 2 3 4A 48 5 6 7 e leature Cowles tog Cowles Bog Dunes naple Transatssion Taas suchgress Foredune osa Dry doses wet woods Creet wones Pone a corridor Cateosters (Conte)

Tenenetontese (eersttag beetles) s

~

i.e.ereits t tavg La retotees R I ne eneriida (EaTiTTriiling twettu)

}pphore sp. I I I

$carabetfee (scarses)

Ataentv1 spp. E  :

CEotru se. I F *2"1 FC**#5 8 FK 2e so. I I Cef e'(le'ghornee beetles)

Ch are e es) I alttet se.

7 ineewalls I nevoaa s,. s ofi M fr_yptocep*alvs sp.

I I

iM ': i

[f sganhe sp. I s

Lema colTarti I 4Tisnote so. I a

Fetar tracnts se,  :

antirRTree T7.ngus .eevets)

I I I s I Cve cultontese (seevils) I I B I I

Aptw sp.

C.Te nore se. a

$c6TriTTae (bert beetles) I nevrooters tantitons. do6sonF11es. etc.)

Coryealidee (dobseafiles, fishfites) I I E a

Chryseoten (genn lecwnes) s a a neaerottidae (brown lacewtags) s a pyvmeleonttese (antitans) 1 I 4ecentere f acoratoaf ties)

Bittactese (mangingfiles)

I Sittecui so.

fetchoptera cadelsflics)

Parygenete (e Senastelg soltat I I

01Fitants so.

LieneenITffee Startssodeg 39 I teetdoetera (butterfites, moths)

Paptltantdae (seellentatt butterflies)

Pa til pt a Pier e (un'tees. pe_ net (miace s=ellowtati) sulfurt)

Cettet pattegico (cocon selfur)  : a s s s PTFig rapee (taportee castagewere) a DeiiTL Tiibeed butterfites) eensos i o m q oneren)  : a sympheTTeae rushfooted butterfitesi Juncata coeste (buckeye) I g

a@ttpuj (etterey)

IEs (pearl creseat) I 2cnie TatYeo attoat$ (question mars) I yegc great meangled frtttilary) 8 I s

anesig el Taata (ree estral)

Selirlies fiatre butterfttu) s t t *ia ty eta (Itttle wood satyr) a et e evrydTcTTey.e tire ==)  :

114 prtTEJig (pearly eye)  :

LycaeaideelT_ves. coopers. hetrstreams) wereg ca*raffi (eastern tattee blue) e caeae us aritoi.vs (artas azure) un carnseverous (htceory hatestreaa)  :

la Jea &TeiaaTi1% ore act><> t s ang>,rees IstwrM 10 plypasagg iie moth ()polyphemus meth) 3 ArtlTides [ tiger moths) net tsedota tesset arts (pale tussock reth)

I Iloctuldee (ceTet 4E underwings) a etela sp. E ojde c.o.eT,i.e. (,eo.etet. .ei.1) so. r

~

I I s o e-tTabov ver .q t emente e 4atI (grepevine looper) I Nira Trofa1Tata I PyreTTime (pyGTTFaitns) i norr2. ie. , .t-taiti i n

rapy ss.

fortrtcIdee (tertrtCtd motas) arc in eareneie a 3 I I I 4*C'r6motsi E t

.)

1-30 science sentoes estvision I

Table 1-19 (Contd)

Sameling $tations 1 2 3 C1 48 5 6 7 8 Inmature Cowles Bog Cowles log Dunes neole fransatssion fasa Beactigrass foredune Oat Dry isoods het Woods Creet lepods pond 8 Corridor Otetera (f1tes) fievlidae (crane flies) A 8 I I I Ptychopteridae (phantom trene flies) esyNc phan tNf a I

s  !

a -tdae tet.

Cha,etortdae tomges) andges) , , , . I. , ,I , 2, otsidae (diste eidges)

Celicidae (mosouttoes) I I I 1 I 1 I mycetoontildae (fungus goats) I A Sciaridae (dartwinged fungus gnats) I I I I I Cecidto=ytidae (gall midges)  : a a I Ceratopogontdee (41 ting midges) X X t I X X X strationvildae (sold * - fuesi i I I P*d ic sp. 1 I us sp. X Te iTseee fitn. ae f1tes) chenen etteato a a Therevida. TIT 11 Tete f1tes)

As114dae (roober f1tes)

Efferia albiberts I Rh s s) I Mystdae (mydas flies)

Pho be caed flies) A I fseldidae (dance f1tes)

C%1opoda sp. I 1 I i sp. I I I A ac ypera sp. I I I DoTTilmoeaTese (Tonglegged files) argyra sp. I C r sotus spp. A I I E 5e Udui sp. a a I to teraus sp. I s 50 8 Ettes sp. I feparitidae (fruit files) 2 Sepsidae (black scavenger flies) ,

La (laumentid flies)

Casytopentopella sp. I I I Emmaeure sp. I IITiit7t ar'sp. s  :

14pr%ra sp. I I I I ptophTTiaae (salpper flies)  : a Sphaerocertdae (dung files) 1 Drosopatlldae (venegar flies)

Ch interoptd flies)

Chlo sp. 3 0

3 sp. E ates spp. I I I I k 259- E Agromyzine (leafeiner flies)

Clustidae (clustid f11es) I Anthonyzidae (anthonyrid flies) 1 Calliphoridae (blow flies) I Nacidae (asssetd flies) I I E a s a a fachtaldae (tecniato flies)

Mysenoptera (bees, wasps, echneumons, etc.)

fontbreelaidae (sawfiles) t tracontdae ibracontds) I I v I Ichnwontdee (tctewons)  :  : - t a fulophtdee (eulophtds) I I Pteromaltdee (pteronaltds)

Scellontdae (sceltor..ds -

a furytomidae (eurytomids' I tvantidae (eastga wasps I X

Nttilidae (velvet sats i I

Foretctdae (ants) I I 1 I 1 X X Speecidae (aud devbers) I

• sisctidae (s.est ben) x Apsae Apis melf tfers (honey bee) I I I E AmphTsoda iscuds)  :  :

X 5 I Chelonetatda chaien,ida (harvest(pseud,oscorpions) en)  : i I Acart (ettes) I I x a a Der =j4 entgr '

Aren.rda rssiderdv riant1tt (Americas dog tict) i t i a i Isopoda (tsopods) 1 Distopoda (eilltpedes) E I I I 1-31 science servloes division

Reticulated beetles are primitive forms that are represented by only five I species in this country and 13 species worldwide. They are little collected, although perhaps not because of rariety but because their habitats are over-f looked (Arnett 1968) . The larvae are woodborers in pine and oak and the adults often remain under bark or in wood as well.

l Velvet ants are actually wasps but are termed ants because the females are f wingless and antiike, and are covered with a dense pubescence (Borror et al.

1976). The group is large - about 470 North American species, mostly distri-buted in the south and west and primarily in dry areas. Appropriately, the single individual observed on the site was in the beachgrass sampling location.

Most species are external parasites of the larvae and pupae of various bees and wasps;_others attack certain beetles and flies.

Mydas flies are large (about 1 inch) black species, sometimes with a distinct orange-gold second abdominal segment. Both larvae and adults of this group ap-parently are predaceous, although little is known about their prey. The larvae live in decaying wood, perhaps feeding on beetle larvae (Peterson 1960). A sin-gle individual of Mydas clavatus, a widespread species in the United States, was observed on the sand along the beachgrass - beach interface.

As in summer 1977, butterfly activity on the site as a whole was good, particu-larly so in the open wooded areas. Not a single checkered white (Pieris proto-dice), which was the most commonly observed species in 1977, was noted. This is probably a function of sampling period rather than presence or absence. Its con-genor, the imported cabbageworm (Pieris rapae), was observed most commonly in open areas (beachgrass, foredune, and transmission locations), as was the common sulfur (Colias philodice) and monarch (Danaus plexippus). The open wooded i.reas harbored satyrs, blues, and hairstreaks particularly, while the several species of brush-footed butterflies observed appeared evenly distributed among the wooded and wood /

field ecotonal areas. The most commonly observed blue was the spring azure (Lycaenopsis argiolus), found in Cowles Bog (wooded) areas; the most common satyr was the eyed brown (Lethe eurydice), which was active in the dry woods of Cowles Bog and along the maple forest / transmission corridor edge; and the most frequently sighted brushfooted species was the great spangled frittilary, found in the dry woods of Cowles Bog and along the foredune. The hickory hairstreak (Satyrium j

carynevorous), which was abundant in the interdunal areas, was observed for the 1-32 solence servlees division

first time on the site; of its known food plants - hickory, black ash, and hawthorn - the latter is the likely host on the site.

1.4.2.1 Beachgrass. Once again the most abundant component of the beach-grass sweepnet sample was delphacid planthoppers. This group was predominant in the July 1976 beachgrass sweepnet sample but not present in that of July 1977. Similarly, the western corn rootworm, extremely abundant on the sand banks in 1977, was not observed in 1978. There is no known relationship between the two species that would explain the rise and fall in abundance of these species. Other prominent insect groups in the 1978 sweepnet sample were leafhoppers, longlegged flies, midges, and false antiike flower beetles. The beetles, although collected from the beachgrass and foredune previously, have not previously been present in such abundance. Food habits of these dark one-fourth to one-half-inch cylindrical beetles are unknown, but sandy areas, including dunes, are reported habitats (Ar-nett 1968). Their attraction to light, as exhibited at the bea:.hgrass and fore-  ;

dune lightcraps, also is reported.

As in 1977, leafhoppers were somewhat more abundant at the beachgrass lighttrap than in the sweepnet sample. The most abundant groups in the lighttrap sample were midges, as expected, and formicine ants. A conspicuous component was a j number of scarab beetles in the genus Ataenius. These small (3 to 5 millipeters) flattened ground dwellers, which are among the detritivorous scarabs, previously were observed to be common on the site in summer of 1974. The beachgrass light-trap also attracted more May beetles (Phyllophaga spp.) than other lightcraps.

These herbivorous light-attracted scarabs are consistently observed at lighttraps on the site although rarely in large numbers. As mentioned previously, their larvae (white grubs) are associated with roots of grasses, particularly lawn varieties. .

Significantly more spiders were collected from the beachgrass in the 1978 sample.

More than half cf them were long-jawed spiders, a group frequently inhabiting fields or meadows adjacent to water. Arthropods extracted from the litter and soil sample were, as usual, comparatively few in number.

The robber fly Efferia albibaris was again observed on the Lake Michigan beach but not in abundance. Insect presence on the beach comparable to the clusters of western corn rootworms and convergent lady beetles of 1977 and blow fly larvae of 1976 was not observed in 1978.

1-33 solence services division

@1.4.2.2 Foredune. The sweepnet sample from the foredune contained the greatest number of insect families in the 1978 samples. Five groups were rep-resented by 20 or more individuals: midges, muscid flies, leafhoppers, aphids, and longlegged flies. Spittlebugs, ants, and mosquitoes were the next most common groups. Among the beetles in the sample were Xylopinus saperdiodes, a medium-sized (12 to 16 millimeter) black darkling beetle that lives under oak bark, and Psenocerus supernotatus, a small (approximately 5 millimeter) long-horned beetle that breeds in a variety of trees and shrubs (Knull 1946), of which only sumac (Rhus aromatica) has been found in the foredune.

Most of the abundant groups at the foredune lighttrap were the same as those at the beachgrass lightcrap: midges, Ataenius, formicine ants, and mosquitoes.

Next to the scarabs, the most common beetle groups at the trap were combclawed beetles and marsh beetles. A single individual of Megalodacne fasciata repre-sented the first observation of the species on the site and only the second ob-servation of a pleasing fungus beetle. Despite a large size (approximately 12 millimeters) and colorful appearance (bla;k and red), this species is rarely seen since it inhabits decaying wood and other fungus-rich habitats.

Litter from the foredune contained on.1y a few more individuals than that from the beachgrass and fewer groups. Th e groups were rove beetles, isotomid spring-tails, ants, and soil mites.

As mentioned previously, a new sma'.1 pool formed next to the cattail area at the base of the foredune (in an area naar where a pool had been located in previous years) and many of the same taxa collected in the past were present this year.

One of the abundant taxa was the unter scavenger beetle Tropisternus lateralis.

The only other beetle collected, Hydrochara obtusata, also a hydrophilid, was not abundant. Predaceous diving beetles were notably absenc since this area pre-viously produced the only significant populations of Laccophilus spp. on the site.

Also absent were water scorpions, although these species also were not collected in other known habitats on the site. Other hemipteran groups, water boatmen, back-swimmers, water treaders, and water striders, were present as before. The most abundant odonates in the sample were coenagrionid damselflies, j

1-34 solence servlees division

o 1.4.2.3 Immature Oak Forest. Midges were by far the most abundant group in the sweepnet sample from these woods. Ants were the second most abundant group, followad by dance flies, spittlebugs, and spiders. Most of the other 35 groups were not abundant, represented by fewer than 5 individual. One-half dozen Cosmopepla bimaculata, mostly larvae, were in the sample. This small (approximately 6 millimeter) black and red stink bug has been consistently present in the oak forest. Like most stink bugs, it is herbivorous and ap-parently is a general feeder that will utilize oak as well as several other trees (Furth 1974).

The lighttrap (and the trappers) in this woods attracted an abundance of mo-squitoes as had occurred under suitable weather conditions in past summers.

Midges, of course, were abundant, as were formicine ants. Those ants, whose winged forms seem to be strongly attracted to light, apparently were nesting on the beach side of the site since none were noted at lightcraps beyond the interdunal areas. Two moths associated with oak were observed: Herculia himon-ialis, a pyralid moth, and Phosphila miseliodes, a noctuid. Both species are widespread and common in eastern and most western United States; the noctuid is strongly associated with Smilax also. The most abundant moth at the trap was the noctuid Epizeuxis aemula, a species associated with fallen leaves; it also is common and distributed over most of this country.

Litter and soil from the oak forest contained the single shortwinged mold beetle observed on the site this sampling period. Also present were larvae of case-bearing leaf beetles, likely a species of Chalmisus, which has been observed in the location in the adult stage. Other groups included pseudoscorpions and millipedes.

1.4.2.4 Cowles Bog (Wooded).

1.4.2.4.1 Dry. The sweepnet sample from the high side of Cowles Bog woods contained the greatest number of individuals of the 1978 sweepnet samples.

Midges, mosquitoes, biting midges, and ants were the most abundant groups, represented by more than 25 individuals. Ntong the next most numerous indi-viduals were crane flies, phantom midges, 15aglegged flies, and darkwinged 1

1 1-35 science services diviolon

fungus gnats. The most abundant beetle, as in the past, was the soldier beetle Cantharis rectus. Ichneumons were, next to ants, the most abundant of several '

hymenopteran groups in the sample.

The litter and soil sample from the dry woods also contained the greatest number of individuals of that sampling category. Among the groups present in the sam-pie were pseudoscorpions, millipedes, rove beetles, featherwinged beetles, and a proturan.

1.4.2.4.2 Wet. In the sweepnet sample from the lower part of Cowles Bog woods, midges were as abundant as in that from the dry woods. The only other group of similar abundance was aphids. Of somewhat lesser prominence were mosquitoes, dance flies, and longlegged flies. Among the Arachnida in the sample, spiders and harvestmen were equally represented, whereas the general ratio in other sweepnet samples was 2 spiders to one harvestman.

The dipnet sample from the wet woods contained only midges in abundance. Typical of past sampling periods, the midge numbers were greater than collected from other aquatic habitats. The other taxa in the sample were mosquitoes, predaceous diving }

beetles, scuds, fishflies, and water boatmen - each represented by either one or two individuals.

As mentioned above, lighttrap activity in this icention was affected by coolness.

Among the comparatively few individuals attracted to the trap vere mosquitoes and midges in greatest numbers and single representatives of gall midges, crane flies, and longlegged flies. The most abundant moth was the grapevine looper, Lygris diversilineata, a consistent visitor to lighttraps in this location.

Arthropods in the litter and soil sample from the wet woods were similar to those collected in past samples, including featherwinged beetles, which generally are found only in the dry and wet woods.

The deer fly Chrysops vitattus was again abundant on the Cowles Bog trail. It seemed to exhibit activity patterns associated with cloud cover and/or relative humidity.

1-36 solence servlees divleion

f 1.4.2.5 Dunes Creek. A variety of aquatic beetles were in the dipnet sample from Dunes Creek. The most abundant, as usual, was the predaceous diving beetle, Hydroporus consimilus. Hydroporus niger was also present, along with Hygrotus sp., the hydrophilids Paracymus and Helophorus, and the crawling water beetle, Haliplus. Several fishfly larvae and water boatmen were in the sample as well as a single backswimmer and giant water bug. As usual, the sample contained phantom crane fly larvae, but their abundance was greater.

1.4.2.6 Maple Woods. The sweepnet sample from the maple woods contained the fewest insect families as well as comparatively few individuals. Spittlebugs and mosquitoes, equally represented, were the only abundant groups present and leafhoppers were the only other group represented by more than 10 individuals.

Among other taxa present, and typically so, were scorpionflies, leaf beetles, click beetles, and lauxaniid flies.

In addition to the perennially abundant scuds, the dipnet sample from the small maple woods tributary to Dunes Creek contained water striders, midges, and phryganeid caddisflies. The caddisfly, Oligostomis ocelligera, lives in long cases constructed of narrow strips of leaf arranged in a spiral. This species is one of the few phryganeids that live only in lotic waters; it probably is restricted on the site to this tributary since the other water bodies, including Dunes Creek, are either ponds or slow streams.

Conspicuous among the comparatively few individuals apptaring at the maple woods lightcrap were the grapevine looper, apparently a common species in all wooded habitats on the site, and another geometrid moth, Scopula imboundata. The latter, a common eastern species, is a general feeder often associated with cherry (Forbes 1948), which is an important plant in the maple woods. Other prominent groups at the lighttrap were midges, mosquitoes, phantom midges, and gall midges.

The litter and soil sample, like that from the foredune, contained comparatively few individuals. The primary difference in comparative numbers of individuals in this and most instances is the difference in numbers of mites, especially soil mites. Among the groups represented in the sample were millipedes, ground bee-ties, and ants.

I 1

1-37 solence services division l

1.4.2.7 Emergent Macrophyte - Pond B. As in 1977, caenid mayflies were the most aoundant group in the cipnet sample from this location. Similar numbers of early instar aeshnid dragonflies were not observed, although their eggs could well have been present during this somewhat earlier sampling period. Coenagrionid dam-selflies were again abundant in the sample as were midges. Aquatic pyralid moths, typically present in the pond, were collected in greater numbers than previously.

One group, snipe flies, which comprises a few aquatic species, was newly' recorded from the habitat.

1.4.2.8 Transmission Corridor. The variety of taxa observed in the trans-mission corridor in 1978 was approximately equivalent to that observed in 1977 and did not approach the greater numbers recorded in 1976 and 1975 as did those of other sampling locations. This could have resulted from herbicide usage along the transmission / railway right of way, although, of course, the sampling does not permit that conclusion.

The seed bug, Ischnodemus falicus, was again the most abundant insect in the sweepnet sample. The plant bug, Trigonotylus tarsalis, which was considerably ,

more abundant than J_. falicus in the 1977 sample, was nearly as abundant as the seed bug in this sample. Aphids, jumping plantlice, anthomyzid flies, and chlor-opid flies were the other insect groups prominent in the sample. These groups also are typically associated with the habitat.

The dipnet sample from the channel adjacent to part of the transmission corridor contained predaceous diving beetles, caenid mayflies, midges, and aquatic mites in abundance. Crawling water beetles, pleid water bugs, and water striders also were prominent components of the sample. Present in few numbers, as before, were water scavenger beetles, libellulid dragonflies, and coenagrionid damselflies.

A single limnephilid caddisfly was collected.

The number of soil and litter inhabitants extracted from the ground sample was not equivalent to those of 1976 and 1977 samples but this was a general observa-tion among the 1978 samples. The groups extracted were typical and, as mentioned above, except for soil mites, were present in approximately equivalent numbers.

l Ground beetle larvae were again more abundant in this ground sample chan others.

i 1-38 soience services divesson

1.5 TERRESTRIAL CITED LITERATURE Arnett, R.H. 1968. The beetles of the United States. Amer. Entomol. Institute, Ann Arbor, MI. 1112 p.

Borror, D.J., D.M. DeLong, and C.A. Triplehorn. 1976. An introduction to the study of insects. Holt, Rinehart and Winston. New York. 852 p.

Knull, J.N. 1947. The 1cag-horned beetles of Ohio (Coleoptera;Cerambycidae).

Ohio Biol. Surv. Bull. 7:133-354.

Furth, D.G. 1974. The stink bugs of Ohio (Hemiptera:Pentatomidae). Ohio Biol.

Surv. Bull. 5, new series. 60 p.

Forbes, W.T.M. 1948. Lepidoptera of New York and neighboring states, Part II.

Cornell Agr. Exp. Sta. Mem. 274. 263 p.

Peattie, D.C. 1930. Flora of the Indiana Dunes. Field Museum of Natural History, Chicago U.S.A.

Texas Instruments IncorporateJ. 1975. First annual report, Bailly Nuclear-1 site, encompassing May 1974-February 1975. Prepared for Northern Indiana Public Service Company.

Texas Instruments Incorporated. 1976. 1975-1976 Annual report, Bailly Nuclear-1 site, encompassing March 1975-February 1976. Prepared for Northern Indiana Public Service Company.

Texas Instruments Incorporated. 1977. 1976-1977 Annual report, Bailly Noclear-1 site, encompassing March 1976-March 1977. Prepared for Northern Indiana Public Service Company.

l 1-39 science services devielen

O SECTION 2 AQUATIC ECOLOGY E

2.1 STATUS Samples were collected for the summer 1978 quarter in August 1978 under the direction of TI's Frank Crawford. Sampling stations are indicated in Figure 2-1. Samples collected, stations, and sample status are shown in Table 2-1.

Dates and purposes of all aquatic field trips during summer 1978 are shown in Table 2-2.

. .n . .ts e i I

} ,C.LE

_ 6 -  % --

4j ~- ._ - s7- -

ws

~

ssy -- _ w. --

- ss 30 y ^-

20 - ___ g 33 " "C f g . g[,,7c'aT._~ 1

• vpat_ - _ - - - - - . _ , .

g

/ . - - . - - _ _

g +z-- ._ , ,

nw W- - - --- g

, .s. y - - --

,o-25 _g-

/ . . .d . .... .. , ... , ,s 4. - ' ' ~ ' '

' ' "'~

/

c=>

m..

N. DUNE Nld

=. ~9y% ' 4I,,, D  :

%  %, ' ' .~ '

/.

= . = ,:. % "' ~ s.j

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%( ,g*.cewers soo7

.., >.- .~u.. . c,...

h,. ~. .

L -._i

{ c..c. . . .. .. ..

.. f.. f -

tocario= wAe Figure 2-1. Map of Aquatic Sampling Stations in Vicinity of Bailly Study Area Showing Stations Sampled for Various Parameters (see Table 2-1)

( Data to be presented herein include phytoplankton, periphyton, zooplankton, ben-thos, aquatic macrophytes, ichthyoplankton, and water quality for June 19~1 as I

well as sediment particle size, water quality, and fishery data for August 1978.

l 2_t science services division l

Table 2-1 3>

Status of Laboratory Analyses as of October 1978,*

Bailly Study Area June August SampiIng Sampi tag M* I F

• Sampling

1. "* #I "

Parameter Sta tion Scheduled Coplete Continuing Scheduied Caplete Continuing" PHYTCPLANETON X X Identification, eneeratton 1 10. 17 21 X X Produc tivity 1 10. 17-21 X X X X Chlorophyll a 1-10. 17-21 X X X X 200PLANTON Identification, eneeratton 1-10, 17 21 X X X X PEX! PHYT 0m Identification, enseratton 1 10, 17-21 X X X X Chloropnyll a, X X X I BDITH05

!dentification, eneeration 1 10, 17 21 X I X X Bottom sediment particle 1-10, 17 21 I I sistag F15H Gill netting 4. 7 X I I X 8each setning 23, 24, 25 I X X X Food habits 1 10 E X*** X X"*

Electroshocker 17.18 I X ICHil1YOPLAMTON'*" 1 10 X X X X mATEX QUALITY General unter quality 1-22 X X X X Aquatic nutrtents 1 22 X X X I Trace elements 13 21 X X X X lndtCators of contamination 11-21 X X X X g ,

Sediment s 12 20 X X ,

AQUATIC MACXOPNVTE5 17-21 X X

• No sampling scheduled in July or Septemoer 1978.

"Continutn9 analyses are presently being caputer-processed and ul11 be included In the Fall 1978 Quarterly Report.

'"The objective of the Food Habits study is to collect 50 spectmens each of 6 tana over the course of 1 year; analyses cannot be performed until all fish are collected.

• • • • 1cheduled with Rooplankton only in August.

Table 2-2 Dates and Purposes of All Aquatic Field Trips, Bailly Study Area, Summer Quarter 1978 Date (1978) Personnel Pararneters Sampled Jul No sampling scheduled; artificial periphyton samplers emplaced Jul 13, 1978 to allow 1-month incubation

! period.

Aug 15-22 Frank Crawford Phytoplankton, Zooplankton

[ Paul Meier Peripnyton, Benthos l Steve Dubois Bottom Sediment Particle Sizing Bill Galloway Fish, Water Quality, Bottom Sediment  ;,

Trace Elements j-

Sep No sampling scheduled i 2-2 soience servloes division

Data currently being pro tssed includes phytoplankton, periphyton, zooplank-ton, benthos, and ichthyoplankton for August 1978. These data will be in-cluded in the fall 1978 quarterly report.

2.2 AQUATIC FLORA 2.2.1 METHODOLOGY. Duplicate 2-liter phytoplankton samples were collected with a 6-liter Van Dorn bottle at stations 1 through 10 in Lake Michigan and at stations 17 through 21 in the pond areas (Figure 2-1). All samples were col-lected from 1 meter below the surface. Before sampling, each 2-liter sample con-tainer was prepared with 20 milliliters of acid Lugol's nolution, which is a nar-cotizing settling agent. After sampling, each container was supplemented with buffered formalin to a final concentration of 4 percent and 3 to 5 drops of liquid detergent to facilitate sedimentation. Before processing, each sample was allowed to settle for 48 hours5.555556e-4 days <br />0.0133 hours <br />7.936508e-5 weeks <br />1.8264e-5 months <br />; then 1800 milliliters of supernatant was siphoned off with a membrane-covered siphon. The remaining 200 milliliters was spun on a laboratory centrifuge at 2000 rpm for 15 minutes to further con-centrate the organisms before the supernatant was filtered off the centrifuge tubes and the " bead" of phytoplankton transfe,rred to 12-dram vials.

In the laboratory, concentrated phytoplankton samples (10 milliliters) were thoroughly mixed and three subsamples were placed in Palmer cells. The al-gae in 12 fields (four per subsample) were identified, enumerated, and meas-ured at 400X magnification. In instances where there was a scarcity of or-ganisms in a sample, the total field count was extended to 24 fields. Bio-volume (microliters per liter) was determined by attributing to the algae geometric shapes best suiting their morphology and calculating their appro-priate volumes (Nauwerck 1963; Rodhe, Vollenweider, and Nauwerck 1958; Strick-land 1960). Instead of developing an average volume / species based on a few representatives, dimensions of each organism enumerated were measured.

Phytoplankton productivity samples were taken at the same locations and sam-pling frequency used for collections for identification, enumeration, and bio-volume measurements. Duplicate samples were collected from 1 meter below the surface at each station in a 6-liter Van Dorn Dottle. Each collected sample 2-3 solence servloes division

@was strained through a 333-micrometer mesh nitex net to remove zooplankters and large detrital material that could be labeled by the carbon-14 material.

The strained water of each sample was measured in a 2-11ter flask to which three 1-milliliter ampoules of 5-pCi NaH 14C03 were added and thoroughly mixed.

A time-zero sample consisting of two 0.5-milliliter subsamples were meas-ured and placed in scintillation vials along with one drop of 6N sodium hy-droxide. Two 50-milliliter subsamples also were removed and strained through Whatman GF/C filters at minimum vacuum pressure (less than 50 milli-meters Hg differential across the filter) and the filters placed in scintil-lation vials. Duplicate clear and darkened 300-milliliter BOD bottles were filled with the remaining sample. When all samples were prepared, they were suspended 1 meter below the surface at their stations for 4 hours4.62963e-5 days <br />0.00111 hours <br />6.613757e-6 weeks <br />1.522e-6 months <br />. Follow-ing incubation, the bottles were retrieved and the contents of each preserved by adding 12 milliliters of buffered formalin. Subsamples of 50 milli 11ters were removed from each bottle and filtered as previously described; each was placed in a labeled scintillation vial with enough tissue solubilizer to cover the filter pad. Activity counts were made using a liquid scintillation counter.

The following formula was used to calculate from the scintillation counts the phytoplankton productivity in milligrams of carbon fixed per liter for each replicate sample:

mg carbon fixed /t/4-hr period = (counting rate / total activity) x (total sample volume /subsample volume) x mg/t initial organic carbon (mg/t) x 1.064 (APHA 1971) where Total activity = amount of potentially available carbon-14 at time zero Counting rate = clear bottle counts minus darkened bottle Counts Total sample = 300 m111111ters volume Subsample volume = 50 milliliters 1.064 = correction for isotope effect J

' 2-4 science services clivision

@Phytoplankton chlorophyll a_ samples were collected from the same water sam-ples from which regular phytoplankton samples were extracted (stations 1 through 10 and 17 through 21). To prepare phytoplankton samples for anal-ysis, a measured volume of water was filtered through a 0.45-micron filter pad stabilized with magnesium carbonate. The filter pad was then frozen for shipment to the central laboratory, where it was extracted for 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> with acetone, ground for 30 seconds with a tissue grinder, centrifuged, and meas-ured on a narrow-band spectrophotometer at 665- and 750-millimicron wave-lengths before and after sample acidification. Periphyton samples were sim-ilarly processed using measured scrapings from natural (as available) or arti-ficial substrates. All concentrations were calculated using the equation:

b - D,) [R/(R-1)] (V/1) (10 /ae) 3 Chlorophyll a_ (ug per sample) = (D

= 11.9 2.43 (Db- D,) (V/t)

(Vollenweider 1974) where D, = optical density of sample after acidification = D665 -D 750 (acidified) 1 D -D b " (ptical density of sample before acidification = D665 750 unacidified) a = specific absorption coefficient for chlorophyll a_ (in grams per centimeter)

V = volume of solvent used to extract the sample (milliliters)

L = path length in centimeters R = D3 /D, for pure chlorophyll a_ = 84 (Talling and Driver 1963)

To convert to micrograms per liter or micrograms per square centimeter, the chlorophyll a_ value was divided by number of liters filtered or number of square centimeters scraped.

Periphyton sampling was scheduled at five Lake Michigan stations (1, 10, 11, 12, and 25) and three pond stations (17, 19, and 21). Pond samples were col-lected using a modification of an artificial substrate sampler first described 2-5 science services division

by Patrick, Hohn, and Wallace (1954). This sampler suspends two racks of 3 five glass slides each, with a surface area of 37.5 square centimeters per J slide, just below the surface as a substrate for periphyton colonization.

Colonization generally occurs in 2 to 4 weeks; thus, the " incubation" time per sampler was 1 month. Lake samples were scraped from natural substrates (as available) at each sampling station. The slides (both sides) and sub-strate scrapings were placed in 8-dram vials and preserved with 6-3-1 solu-tion.* Two replicate slides per sample were quantitatively analyzed; however, all slides were scraped and the scrapings saved for references. Counts were made as described for the regular phytoplankton samples. Biovolume estimates were also generated in a manner fashioned after the phytoplankton biovolume technique.

2.2.2 RESULTS AND DISCUSSION - PHYTOPLANKTON 2.2.2.1 Identification and Enumeration. Average Lake Michigan phytoplank-ton densities during June 1978 were 6,211

• 1,873 cel'.s/ milliliter, while near-shore pond densities averaged 9,771 i 7,788 cells / milliliter (Table 2-3; Appen-dix A . This represents an increase from April 1978, when lake and pond den- )

sities averaged 5,795

• 1,682 and 5,199

• 2,365 cells / milliliter, respectively.

Biovolumes for Lake Michigan averaged 2.08 i 0.39 microliters/ liter, while bio-volumes for the ponds averaged 5.67

• 4.58 microliters/ liter (Table 2-4; Appen-dix B). These values compare with 5.8

• 1.9 microliters/ liter for April 1978 Lake Michigan samples and 7.7

• 5.4 microliters/ liter for April 1978 interdunal pond samples.

Dominant taxa in Lake Michigan (greater than or equal to 4 percent of either density or biovolume) included 04cillatoria sp., Aphanizomenon fios aquae, Fragilaria sp., and Tabellaria flocculosa. Dominant taxa in the ponds (using the same criterion) included Microcystis sp., Lyngbya sp., Crucigenia rectangu-laris, and Cryptomonas sp. Bluegreen algae comprised 55 percent of the density but only 11 percent of the biovolume, while diatoms contributed 77 percent of the biovolume.

6 parts water: 3 parts ethanol: 1 part of formalin. ,

2-6 solence services division

Table 2-3 Phytoplankton Density, Bailly Study Area, June 1978 nunaER cr CEttS PER LITE" Lake Michigan Stations Nearfield Mean Farfield LS TAXA 1-6 & 10 7-9 -

X S.E.

REL ABZ -

0 0 0 UttIDEtITIFIED ALGAE 90500.19 196650.44 143575.31 53075.12 2.3 0 Lil!OEtlTIFIED ALGAE ELFILI 90500.19 196650.44 143575.31 53075.12 2.3 0 CYAt:3PtiTTA 27033S9.00 4195376.00 3449632.00 746243.50 55.1 0 Citn00COCCACEAE O t11CROCTSTIS 0 11ICROCVSTIS ILPILI 0.0 234126.81 117063.37 117063.37 1.9 0 OSCILLATC9IACEAEi 0 OSCILLAT03!A 0 CSCILLATCRIA (LPIL) 1899978.00 2630550.00 2065 64.00 365086.00 36.2 0 lyt:C0YA 0 Lit:3t A E LPIL) 418:34.6 334467.00 376350.81 41883.81 6.0 0 OSCILLAT031ACEAE tLPIL) 2009.73 0.0 4004.87 4004.87 0.1 0 f:DSTOCACEAL 0 At!.*,C A t tit 0 Att/D AEt:A ( LPIL) 4445.40 0.0 22:2.70 2002.70 0.0 p 0 ArilAtlIZCitit:0 4

$ 0 0 CHLOCCPHYTA FLOS-AQUE 372721.00 215633.19 9*6731.62 315344.62 684706.31 31:005.31 10.9 265463.87 49355.72 4.2 0 VOLVOCALES 0 CllLAt1YD0t*0!!AS 0 Ct!LArnPO:10l1AS ( LPIL) 21613.94 4685.70 13149.82 8464.12 0.2 0 VCLVC0 ALES (LPIL) 18342.30 0.0 9171.15 9171.15 0.1 0 TETP LSPCR AL E S 0 GLCEOCVSTIS 0 GLOECCTSTIS (LPIL) 1481.80 0.6 740.90 740.90 0.0 0 ELAKATOTitRIX 0 ELAt;ATOTH7IX (LPIL) 0.0 9611.69 4835.84 4805.84 0.1 e O CitLO2CCCCCALES 0 0 At:KISTRCDES;?JS e O CC:tVOLUTUS 4032.46 0.0 2041.23 2041.23 '0. 0 3 0 FALCATUS 24163.64 14840.73 19504.68 4663.95 0.3 O O At: KIST 3C0!St1US (LPIL) 6407.79 0.0 3:03.89 3003.89 0.1 0 CCCYSTIS

1. 0 COCISTIS (LPIL) 3344.67 0 0.0 1672.33 1672.33 0.0 0 GOLEt:KIllIA 0 GOLEIC:IllIA ILPIL1 0.0 3604.38 1802.19 1200.19 0.0 0 0 MICRf CTIt41Ut1 0 0 t11CRACTIll!Utt (LPIL) 26031.64 0.0 13015.82 8 13015.80 0.2 0 01CT f 03."!!' ECIUrt

& 0 DICT)C Ult.*ERIUr1 ILPIL) 4565.55 0.0 2:22.78 2282.78 0.0 4 0 SCEt:IDESt?JS 3 3 ACUttillATUS 12244.89 91436.56 51840.73 39595.83 0.8 0

3

Table 2-3 (Contd)

HUMSER OF CELLS PER LITER gg gg gg SITE

SUMMARY

g3g Nearfield Farfield 1-6 & 10 7-9  ; 3,E, "$

tS TAXA 0 0 7485.10 7208.77 7346.93 138.17 0.1 0 SCEt:EDEStiUS (LPIL) 0 T ETR AE0f'Ott 8362.68 8361.68 0.1 0 t:UTICOM 0.0 16723.35 0 CRUCIGEtlIA 5626.91 5686.91 0.1 0 QUADRATA 11373.82 0.0 35683.37 167233.50 101458.44 65775.06 1.6 0 CHLCRCCCCCALES (LPIL) 0 ULOIRICHALES 0 ULO1HRIX 5706.94 5706.94 0.1 0 ULOIHRIX (LPIL) 11413.87 0.0 13696.66 27393.31 0.0 13696.66 0.2 0 ULOTRIC!t ALES ( LPIL) 21584.92 9052.33 0.3 0 XAt4THOPHY T A 12532.59 30637.25 0 HETER 0TRICHALES 0 T RIGot,EttA 0.1 12532.59 0.0 6266.29 6266.29 0 TRIE0 NEMA (LPILI 0 RHIZOCHLC91 DALES 0 STIPITOCOCCUS 15318.62 15318.62 0.2 0 STIPITOCOCCUS (LPIL) 0.0 30637.25 182955.56 220585.50 201770.50 18814.97 3.2 bJ 0 CHRYSOPHYTA b 0 0

CI:RYSOMC:iADALES DII;OO'tYCt1 0.0 1955.78 977.89 977.89 0.0 0 SERTULARIA 11328.77 34552.00 11894.46 23223.23 0.4 0 DIVERGEt:5 3344.67 33 % .67 0.1 0 CYLItCRICUM 0.0 6689.34 10354.61 32139.03 21246.84 10892.23 0.3 0 SOCIALE 4715.89 0.0 2357.94 2357.94 0.0 (

0 DIt:CCRToti ( LPIL1 0 EEPH1RIOtt 12715.46 0.2 25430.92 0.0 12715.46 0 KEPHYRI0ti (LPILI 0 CHRYSCCH20t1ULIt!A 0.1 13696.66 0.0 6848.33 6848.33 g 0 PARVA 1.2 89499.01 55209.81 72354.31 17145.00 0 0 CHRYSct:0:140 ALES (LPIL) g 0 NCt:0SIGALES 3 0 t*0:10SIGA 55477.44 1742.12 112697.00 57219.56 0.9 0 0 :ct:0SIGA (LPILi 1481.80 1481.80 0.0 0 0 CHRYSOP:tVTA (LPIL) 2963.60 0.0

1. 67572.44 242883.94 155228.19 87655.75- 2.5 l 0 t3 ACILLARICPilY T A-CENTRIC l 0 0 EUPODISCALES 0 8:ELOSIRA 370.45 740.90 0.0 370.45 0.0 -

O O ITALICA 86.35 ISLAtcICA 9183.66 9010.96 9097.31 0.1 S 0 8 0 SNELETC::EttA 0.0 62475.98 31237.99 31237.99 0.5 CL 0 POTAt:US 47789.88 140145.19 93967.50 46177.65 1.5 0 EUPODISCALES ( LPIL)

{

O -

3 l

-( te d-

Table 2-3 (Contd) wutzER or CEtts PER LITER Lake Michigan Stations Mean flearfield Farfield REL LS TAXA 1-6 & 10 7-9 X S.E. AS%

0 0 0 RHIZOSOLENIALES 0 RHIZOSOLENIA 0 ERIENSIS 9557.98 31251.81 20554.90 10696.91 0.3 0 BACILLA210PHfTA-PENT 4 ATE 993457.37 2561898.00 1777677.00 784220.31 28.4 0 FRAGILARIALES 0 ASTERIONELLA 0 FORit35A 6145.12 84305.62 45225.37 39080.25 0.7 0 DIATO:tA 0 TE:UE 22115.04 344843.25 183479.12 161364.06 2.9 ,

O FRAGILARIA

• 0 CROT0t:!t: SIS 66769.12 96306.62 81537.87 14768.72 1.3

! O TR AGIL ARIA ( LPIL 367091.25 1546063.00 956577.12 589485.87 _15.3 0 S)HID2A I O ULHA 568.69 1441.75 1005.22 436.53 0.0 1 0 Sit:ED2A (LPIL) 79955.94 22907.20 51433.07 28525.87 0.8 0 TACE LLA RI A .

O F Et:ESTR ATA 20636.79 0.0 10318.39 10318.39 0.2 y 0 FLOCCULOSA 242528.56 366934.75 304731.62 62203.09 4.9 i us 0 TABELLARIA (LPIL) 42666.23 0.0 21333,12 21333.12 0.3 i 0 FRAGILARIALES (LPILI 24023.24 0.0 12011.62 12011.62 0.2 0 "AVICULALES 0 flAVICULALES ELPIL) 9834.03 11706.34 10770.18 936.16 0.2 0 CSCILLARIALES 0 flITZCHIA 0 ACICULARIS 41282.17 14297.39 27789.78 13492.39- 0.4 0 IIIT2 CHI A E LPIL) 42632.61 37226.06 39929.33 2703.27 0.6 0 SURIPELLALES 0 SURIRELLA g 0 SU2IRELLA ( LPIL 3 3061.22 0.0 1530.61 1530.61 0.0 0 0 SACILLARIorHYTA-pet 3l&TE (LPILI 24144.91 35866.30 30005.61 5860.69 0.5

{ 0 Pt RRHOPHYT A-O ttioPHYCE AE 2267.83 0.0 1133.92 1133.92 0.0

.3 0 G)ft ODIt:I ALES O 0 Gft;.t3ItIIUit 0 0 GY1C.VOItllutt t LPIL3 1481.80 0.0 740.90 740.90 0.0 W 0 PERIDI!11 ALES O O PERICIt:IUM 2 0 II:2Ct:SPICUUN 200.24 0.0 bd.12 100.12 0.0 I 0 FEPIDINIUtt iLPIL) 585.79 0.0 292.89 292.89 0.0 0 0 CRYPTCP:4YTA 119752.19 370471.19 245111.69 -125359.50 3.9 8 0 CR T FTCC:03 A LES

& O CRY PTC'!O!4 AS 0 t1ARSS0t41I 2983.63 0.0 1491.81 1491.81

{ 0.0 e

0 3

Table 2-3 (contd) tCMBER CF CELLS PER LITER Lake Michigan Stations an Nearfield Farfield LS TAXA 1-6 & 10 7-9  ; 3,,, %I l 0 0 i

1 0 CRYPT 0t10 MAS (LPIL) 14821.80 15378.70 15100.25 278.45 0.2 0 RHODCI" alas 0 ttIttuTA 66901.31 69425.75 68163.50 1262.22 1.1 0 LEllS 4565.55 7208.77 5887.16 1321.61 0.1 0 CHRCOMCt1AS 0 CHROOMOtl4S ( LPIL) 21296.29 0.0 10648.14 10645.14 0.2 0 CYAICMC!a4S 0 CYAllutiO!IAS f LPIL) 0.0 6519.27 3259.64 3259.64 0.1 0 CRtPictCIADALES (LPIL) 9183.66 271938.69 140561.12 131377.50 2.2 TOTAL 4386055.00 8134341.00 4261193.00 1873143.00 100.0 OIVERSITY IH PRIrl!) 2.47 2.30 2.39 0.08 DIVERSITY (J PRIt:El 0.66 0.60 0.63 0.03 ffJM3ER OF TAXA SS 40 67 ACOVE cot 1PUTED USIt:G SAMPLE IDS 11 12 21 22 y 31 32 52 41 61 42 62 H 51 0 101 102 71 72 81 82 91 92 0

0 e

3 6

e e

e 2

o o

e IL 5

e 0

3

( L' -

Table 2-3 (Contd) t.trBER OF CELLS PER LITER SITE SurmRY Pond Stations Mean tS 7Axa 17-18 19-20 21 x S.E. NIe 1 1 1 0 tki!DEliTIFIED ALGAE 22707.59 0.0 3760544.00 1127750.00 1116415.00 11.5 I

O tri!DEtITIFIED ALGAE (LPIL) 22707.59 0.0 3360544.00 1127750.00 1116415.00 11.5 0 CYA!.VFH1TA 237168.37 401985.56 12843658.0 4494270.00 4174964,00 46.0 ,

O CliRCC00CCACEAE I O ttICROC)STIS I O ttICROCYSTIS (LPIll 0.0 0.0 6166791.00 2055597.00 2055597.00 21.0 0 Cot 1FHOSTHAERIA '

O C0!trH03FHAERIA (LPIll 237168.37 251225.44 0.0 162797.94 81500.00 1.7 0 OSCILLATORIACEAE O OSCILL ATO'?I A 0 CSCILLATCRIA (LPILI 150760.31 50253.44 f 0.0 0.0 50253.44 0.5 '

O Lit:CGT A 0 LYl:33YA (LPILI 0.0 0.0 6676570.00 2225623.00 2225623.00 22.8 0 CHLO73ntVTA 15S9450.C0 969124.50 67C0850.00 3026484.00 1816047.00 31.6 0 VOLVCCALES 0 CHLAt11DCit0ltAS 0 CHLAt1100t:0:4AS (LPILI 0.0 12254.90 0.0 4004.97 4084.97 0.0 N 0 GC!1!Utt h

H 0 CO:4Ittt ( LPIL ) 0.0 126288.50 0.0 42096.16 42096.16 0.4 -

0 RADIOFILtR1 0 RADIOFILU:1 (LPIL) 0.0 503938.56 0.0 169646.19 169646.19 1.7 0 VOLVCCALES iLPILi 17391.15 0.0 0.0 5797.C5 5797.05 0.1 0 TETR?.SFCRALES .

O GLOZOCYSTIS 0 GLCECOTSTIS (LPIL) 95966.62 0.0 0.0 3193S.87 31988.87 0.3 0 CHLO7C0000 ALES 0 SF11AEROO1STIS 0 SFil2EDOOYSTIS (LPILI 702494.12 0.0 245098.06 315864.06 205856.19 3.2 0 At:KISTRCCESP.JS S 0 CCt.".'CLUTUS 9010.96 0.0 0.0 3003.6S 3003.65 0.0 0 0 FALCATUS 0.0 0.0 CS435.37 29 *+78.46 29478.46 0.3 0 0 CLOSTERIOPSIS

'3 0 CLCSTERIGPSIS (LPIL) 6055.36 13180.27 88435.37 35890.34 26352.90 l 0 0

KIRSCt:t: ERIE LLA LutaARIS 97002.94 0.0 0.0 32334.31 32334.31 0.4 0.3 lg 0 o

-00 CYSTIS DOC)STIS (LPIll 26402.10 91911.69 0.0 39437.93 27321.47 0.4 O CCEti 0E01:US O O G'J ADRIC AUD A 279609.94 0.0 0.0 93203.31 93203.31 1.0 l 0 0

SPII:05US SCEl:10ES:1;S (LPILI 36900,94 86S65.56 0.0 10993.37 0.0 0.0 12326.9S 32619.64

- 12326.98 27307.98 0.1 0.3 k 0 PEDIASTRutt i

5 0

3

Table 2-3 (Contd) tAEGER CF CELLS PER LITER SITE SumARr Pond Stations Mean LS TAXA 17-18 19-20 21 E S.E. "S A

1 1 1 0 TETRAS 0.0 24509.80 0.0 8169.93 8169.93 0.1 0 TE TR AECR0tl 0 CAUDATuti 0.0 5496.68 0.0 1832.23 1832.23 0.0 0 t1UTItutt 0.0 0.0 88435.37 29478.46 29478.46 0.3 0 ftINITL*1 0.0 5496.68 0.0 1832.23 1832.23 0.0 0 CRUCIGEt!!A 0 RECTAt4GULARIS 0.0 0.0 1414966.00 471655.31 471655.31 4.8 0 COELASTRUt1 0 MICR0P0FUM 0.0 98039.19 0.0

• 32679.73 32679.73 0.3 0 CHLOR 0CCCCALES ILPIL) 206531.00 6127.45 ' 4775510.00 16627 2.00 1557463.00 17.0 0 ZYCHEt1ATALES 0 C0!ilAR1Ut1 0 CCSttARIUti (*.PIL) 0.0 46581.12 0.0 15527.04 15527.04 0.2 0 HYALOTHECA 0 HTALOTHECA ( LPIL) 25140.56 0.0 0.0 8380.19 8380.19 0.1 0 STAURASTRUt1 N O PARADO:31 13180.27 0.0 0.0 4393.42 4393.42 0.0 h

N 0 J0:n:SCtlII 0.0 6127.45 0.0 2042.48 2042.48 0.0 0 EUGLEll0PHYTA 0.0 6127.45 30637.26 12:54.90 9359.84 0.1 4 EUSLEttALES 0 EUOLEt!A 0 EUGLE!A (LPIL) 0.0 0.0 30637.26 10212.42 10212.42 0.1 0 TRACHEL0tCilAS 0 TRACHELCt10t!AS ( LPIL) 0.0 6127.45 0.0 2042.43 2042.48 0.0 0 CHRISCFHYTA 7569.20 12254.90 207508.00 75777.31 65879.19 0.8 0 CHRISCt10tlAD A L E S 0 CHRYSCCOCCU3 0 CHR100CCCCUS (LFIL) 0.0 0.0 30637.26 10212.42 10212.42 0.1 0 CHRISC;'.Ot!AMLES I LPIL) 7569.20 12254.90 176870.75 65564.94 55669.33 0.7 g 0 BACILLAR 10FHYTA-CENTRIC 24960.35 10544.22 503451.37 179651.94 161953.12 1.8 0 EUTCDISCALES

!0 O 0

tiELCSIRA MELOSIRA (LPIL) 0.0 0.0 30637.26 10212.42 10212.42 0.1 0 0 EUFDDISCALES (LPIL) 24960.35 10544.22 472814.12 169439.56 151744.31 1.7 3 0 8 ACILL ARICrit V T A-pef otATE 98967.25 271617.50 1395365.00 58S649.87 406425.00 6.0 0 0 FRAGILARIALES 2 0 ASTERIONELLA

{ 0 F 02ft3S A 8380.19 55357.12 0.0 21245.77 17226.38 0.2 g 0 DIAT0ftA S 0 TEt:UE O.0 0.0 9191.18 3063.73 3063.73 0.0 g 0 FRAGILARIA 0 FRAGILARIA (LPILI 26194.85 183598.37 30637.26 80143.44 51743.33 0.8 f.

0 2

( v M

1 i

Table 2-3 (Contd) tAJMSER OF CELLS PER LITER SITE Sut m Rr Pond Stations tiean LS TAXA 17-18 19-20 21  ; 5.E. "S A

1 1 1 0 SINEDRA 0 5)l4EDR A ( LPIL) 0.0 2450.98 0.0 816.99 816.99 0.0 0 FRAGILARIALES (LPIll 0.0 0.0 88435.37 29478.46 29478.46 0.3 0 Eut40TIALES 0 ELR 0TI A 0 EUt:0TIA (LPIll 6704.15 0.0 61274.50 22659.55 19404.23 0.2 0 ACHtIAt4TilALES 0 ACHilAPITHES 0 ACH!IAtITHES (LPILI 20887.82 0.0 0.0 7629.27 7629.27 0.1 0 COCCO*EIS 0 CCCCO'4EIS (LPILI 0.0 13180.27 0.0 4393.42 4393.42 0.0 0 14AVICUL AL E S 0 HAVICULA l 0 flWICULA (LPILI 0.0 5406.57 387224.81 130877.12 1 8183.31 1.3 0 pit 33JLARIA 0 PItalULARI A (LPIL) 0.0 0.0 15318.63 5106.21 5106.21 0.1 9 0 GottritCt;EttA 4 0 CO:1PH0!1Et14 (LPILI 0.0 0.0 30637.26 10:12.42 10212.42 0.1 C 0 titvICULALES ( trILI 0.0 0.0 207508.00 69169.31 69169.31 0.7 0 BACILLARIALES 0 HA!ITZSCHIA 0 Htt:TISCHIA (LPIL) 0.0 6127.45 0.0 2042.48 2042.48 0.0 0 t41TICli! A 0 flITZCHIA (LPILI 9010.96 0.0 23C145.25 80385.37 77923.31 0.8

'0 3URIRELLALES 0 SLGICELLA 0 SURIRELLA (LPILI 7569.20 0.0 0.0 25:3.07 2523.07 0.0 0 BACILLARIOPHYTA-Peti!! ATE (LPIL) 18220.15 5496.68 326993.25 116903.31 105109.12 1.2 0 P)REl:OrH YT A-DIHOFHYCE AE 901.10 32025.31 0.0 10975.46 10528.13 0.1

1. 0 FERIDIt4IALES O O PERICIllIL:M 8 0 G4 tut 1EttSE 901.10 6127.45 0.0 2340.85 1910.10 0.0

$ 0 PERIDitlIUN ( LPILI 0.0 25397.86 0.0 8630.62 8632.62 0.1 t) 0 CRYPTCFetYTA 224S23.31 53127.66 302896.12 195 80.31 72185.81 2.0 g 0 LRYPTOMON00&tf5 g C CR f PT0*:Ot4AS

] 3 ttARSSO:4II 8650.52 0.0 0.0 2893.51 2883.51 0.0 0 CRIPTCita:4AS ( LPIL) 0.0 18586.25 214460.75 77630.50 68599.05 0.8 0 KHODOtt014AS e 0 f tItAJT A - 216172.75 13180.27 0.0 76451.00 69964.37 0.8 O CHR30t Ot4A5 g:. CHROct:OtAS (LPILI 26360.55

- O 0.0 0.0 87c6.85 8786.85 0.1 1

o.

3

Table 2-3 (contd) i NU'1BER OF CELLS PER LITER SITE Sur 1ARY ,

Pond Stations Mean LS TAXA 17-18 19-20 21 x 5.E. "$

Ae 1 1 1 0 CRYPT 0t10tIADALES (LPIL) 0.0 0.0 88435.37 29478.46 29478.46 0.3 i TOTAL 2206544.00 1761805.00 25344848.0 9771065.00 7787949.00 100.0 DIVERSITY (H PRIt1El 2.58 1.98 3.03 2.53 0.30 DIVERSITY (J FRIME) 0.73 0.65 0.76 0.71 0.03 IAJtSER OF TAXA 26 31 26 59 ABOVE C0t1PUTED USit:G sat 1PLE IDS 171 172 181 182 191 192 201 202

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i 2 21 solence servlees division L.

Table 2-4 (Contd)

1. 11CROLITERS PER LITER SITE ss w RY ~

Pond Stations Mean REL LS TAXA 17-18 19-20 21 x S.E. Asx 1 1 1 0 CRYPTCTIONADALES E LPILI 0.0 0.0 0.01 0.G0 0.00 0.1 TJTAL 0.39 1.82 14.79 5.67 4.58 100.0 OIVERSITY (H PRIME) 2.10 1.48 1.93 1.c4 0.19 DIVERSITY (J FRIME) 0.61 0.51 0.4S 0.54 0.04 IAJME.ER OF TAXA 26 31 26 59 ABOVE C0ftPUTEd USItG SAttPLE IOS 171 1 72 131 182 191 192 201 202 I

til 212 w

I i N N

1 4

e 0

9 3

0 e

8 a

i G

e E

e I

3

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O e

A total of 67 taxa were observed in Lake Michigan samples and 59 in the inter-dunal pond samples. Due to the greater number of taxa in the Lake Michigan samples, diversity (H') was higher in the Lake Michigan samples. The Evenness (J') index was higher for density in the nearshore ponds but was higher for biovolume in Lake Michigan.

Within the nearshore ponds, density and biovolume were highest (25,345 cells /

milliliter and 14.79 microliters/litar, respectively) in Cowles Bog, just the opposite of April 1978 when the density and biovolume were lowest in the Cowles Bog samples. A total of 26 taxa were observed in the Cowles Bog samples, an in-crease of 4 from the April 1978 sampling. Pond C samples yielded the lowest mean density (1,762 organisms / milliliter) while Pond B samples yielded the low-est biovolume (0.39 microliter / liter). All values were within ranges observed in previous years.

In general, density, biovolume, diversity, and number of taxa mirrored previous years. The major spring diatom pulse appeared to have passed in Lake Michigan, although diatoms still contributed 30.9 percent of the density. As in pre-vious years, the larger, yet less numerous, diatoms continued to be a major factor in the Lake Michigan biovolume. Within the nearshore ponds, the flora was dominated numerically by green and blue-green algae and volumetrically by the cryptophytes.

2.2.2.2 Chlorophyll a. Concentrations (micrograms / liter) of chlorophyll jL are shown in Table 2-5. As indicated, values ranged from 0 to 34.7 micrograms /

liter. The average chlorophyll jt value in Lake Michigan samples was 5.790 mi-crograms/ liter, while the average pond value was 4.944 micrograms / liter. With-in the pond stations, Pond B samples (stations 17 and 18) which yielded the lowest biovolume, also yielded the lowest chlorophyll a levels in the June sam-pling period. Although Cowles Bog samples had high biovolume, the biovolume was dominated by cryptophytes and yielded lower chlorophyll it levels than Pond C (stations 19 and 20) biovolume which was dominated by green algae and pyr-I

, rophytes.

l 2-23 solence servloes division

Table 2-5 Chlorophyll a Concentrations (pg/1) Recorded from 15 Phytoplankton Sampling )

Stations in the Bailly Study Area, April 1978

~5tation Peelicate (a) ,e,iicIto (e) 1 16.696 7.220

$ l'd$ $5

4% tro

' tit? 1.r Ln F.806 Hi:

7.00 10

• *a 9,190 0 li:$'d I* '

1: :1:!!!! 3M:

tt 9.821 S . 7t!

Nan 4,94d 2.2.2.3 Productivity. Productivity, as measured by 'C-fixation at 15 phytoplankton sampling stations, yielded the results shown in Table 2-6.

Values were low, as they had been in April, with an average fixation rate of 0.005 milligram carbon / liter / hour in Lake Michigan and 0.023 milligram car- '

bon / liter / hour in the nearshore ponds. Both mean values were lower than those recorded in June 1977. However, none of the data appear to indicate an ad-verse effect of power plant operation, particularly as the value at Station 10 (Bailly Station discharge) was slightly higher (a stimulatory effect) than other Lake Michigan values.

2.2.3 RESULTS AND DISCUSSION - PERIPHYTON 2.2.3.1 Density and Biovolume. Average Lake Michigan periphyton densitics 6

during June 1978 were 5.25 x 10 cells / square centimeter (Table 2-7 and Appen-dix C) with an average biovolume of 1.13 microliters/ square centimeter (Table 5

2-8 and Appendix D). Pond densities were lower (1.68 x 10 cells / square centi-uctar) (Tabic 2-7 and Appendix C), as was biovolume (0.14 microliter / square centimeter) (Table 2-8 and Appendix D). Diversity was lowest for the Lake Michigan stations at Station 12, which is located on the northeast corner of the 3ethlehem Steel Breakwater and on the fringes of the observed extent of the Bailly discharge plume. It is not thought that plant operation or con-struction caused the low density and diversity, since stations closer to the ,

discharge yielded far greater density and greater numbers of taxa.

2-24 solence servlees diviolon

O I

Table 2-6 ,

Carbon Fixation (mg/t/4-hr incabation)* Recorded at 15 Sampling Stations in Bailly Study Area, June 1978 Station Replicate (a) Replicate (b)

Lake Michigan 1 No sample ** 0.030 2 0.023 0.006 3 0.017 0.0005 4 0.007 0.025 5 0.025 0.023 6 0.014 0.009 7 0.017 0.025 8 0.014 0.024 9 0.028 0.011 10 No sample ** 0.076 Nearshore Ponds 17 0.235 0.169 18 0.168 0.165 19 0.019 0.062 20 0.006 0.070 21 0.002 0.016 All values calculated using the equation:

Counting Rate 300 mgC/t/4-hr period " Total Activity

• Volume x mg/tc x 1.064 Filtered No sample - container cracked in shipment.

Distinct differences were noted between Lake Michigan and pond periphyton com-position during June 1978. Lake Michigan periphyton was numerically dominated  ;

by blue-green algae (Cyanophyta), which comprised 98.3 percent of the density f and 82.6 percent of the biovolume. The samples were dominated by Oscilla- l toria sp. , Lyngbya sp. , and Calothrix sp. Nearshore pond periphyton was dom- j l

inated by Chlorophyta (green algae) and Bacillariophyta-pennate (pennate dia-tons). Dominant taxa (greater than or equal to 4 percent of either density or biovolume) were Lyngbya sp. , Scenedesmus quadricauda, g aracium ambiguum, Oedogonium sp. , Svnedra sp. , Achnanthes minutissima, and Epithemia turgida.

2-25 science services division

Table 2-7 Periphyton Density, Bailly Study Area, June 1978 l

DENSITr IN HUME,ER / SQUARE CENTI?tETER STATICH REPCRT gggn 3 7,x, Lake Michigan Stations x S.E. A 1 10 11 12 25 .

O LH10ENTIFIED ALGAE 644892.00 0.0 0.0 0.0 128978.37 128978.37 0.1 0.0 0 UNICEtiTIFIED ALCA! (LPIL) 644892.00 0.0 0.0 0.0 128978.37 128978.37 0.1 0.0 0 CYAtCPHYTA 294664448. 31405920.0 4818:00.00 65675255.0 89124603.0 52364512.0 98.3 49056224.0 0 CSCILLATCRIA.CEAE O OS21LLATORIA 197629596. 0.0 0.0 1899290.00 40004320.0 39407872.0 44.1 492678.53 0 CSCILLATORI A (LPIL) 197629676. 0.0 0.0 1899290.00 40004320.0 39407872.0 44.1 to 492678.56 I

$ 0 LYtGSTA 960674$3.0 48563552.0 14865713.0 4818:00.00 63778976.0 45618784.0 16574038.0 50.3 0 LYtG8YA (LPILI 960o74S8.0 14865713.0 4318200.00 63770976.0 4561$784.0 16574033.0 50.3 48563552.0 0 RIVULARIACEAE O CALOTHRIX 0.0 16540218.0 0.0 0.0 330S043.00 3305043.00 3.6 0.0

1. 0 CALOTHRIX (LPILI 0.0 16540218.0 0.0 0.0 3308043.00 3308043.00 3.6

!. 0.0 Ig 0 CYAtCPHYTA (LPILI  % 7333.00 0.0 0.0 0.0 193467.56 193467.56 0.2 g 0.0

.lg. 0 CHLOROPHYTA 644892.03 0.0 506334.25 239943.31 231385.44 324511.00 113321.44 0.4 0 CHLCRC"0CCALES g 0 SCENEDEst105 644892.00 506334.25 0.0 0.C 230245.25 142637.69 0.3 g 0.0

$' 0 ouAoRICAuoA 0.0 473045.50 0.0 0.0 '94609.06 94609.06' O.1

e. 0.0 o.

3 -

v. Q.

Table 2-7 (Contd) o DEllSITY Il4 PAA10ER / SCUARE CE?iTIt'ETER STATI0tl REPCRT Mean LS TAXA Lake Michigan Stations -

REL X S E. AS%

1 la 11 12 25 0 ECO2t;IS 0.0 33286.75 6657.75 0.0 0.0 6657.75 0.0 0.0 0 SCENECES!1US ILPIL) 644892.00 0.0 0.0 0.0 123978.37 128978.37 0.1 0.0 0 ULOTRICHALES 0 ULOTHRIX 0.0 0.0 239943.31 231385.44 94265.75 57741.60 0.1 0.0 0 U10 THRIX ( LPIll 0.0 0.0 239943.31 231335.44 94265.75 57741.60 0.1 0.0 0 BACILLARIOPHYTA-CENTRIC 322446.06 0.0 37370.53 0.0 71963.31 63337.46 0.1 ta 0.0 dN 0 EUPOOISCALES 0 EUFCDICCALES ( LPIL) 322446.06 0.0 37370.53 0.0 71963.31 63037.46 0.1 0.0 0 DACILLARIOPHYTA-FEt#1 ATE 789294.75 151550.12 2627C63.00 139756.75 1043959.00 470539.31 1.2 I 1537135.00 '

0 FPAGILARIALES 0 DIATOMA 59130.69 2076924.00 0.0 0.0 562900.00 399569.50 0.6 l 678445.44 I l

e 0 VULGARE 0.0 0.0 37370.53 7474.11 0.0 7474.11 0.0 0.0 e {

lg 0 TENUE 0.0 678445.44 59130.69 2039553.00 0.0 555425.81 392496.56 0.6 g 0 FRAGILARIA 176647.25 0.0 0.0 53350.84 45999.62 34256.92 0.1 l J 0.0 f

g 0 VAuCHERIAE 176647.25 0.0 0.0 0.0 53356.84 45999.62 34256.92 0.1 b 0 SYl4EORA 0.0 0.0 58980.75 0.0 11796.15 11796.15 0.0

f. 0.0 e

0 3

\

Table 2-7 (contd)

DEt4SITY III IAJMBER / SQUARE CENTIMETER STATICH REFORT g Lake Michigan Stations - REL LS TAXA X S.E. AS%

1 10 11 12 25 0 SYt1EDRA (LPIL) 0.0 0.0 58980.75 0.0 11796.15 11796,15 0.0 0.0 0 ACHtIAtiTHALES 0 ACHNANTHES 290201.44 0.0 63895.81 0.0 169355.12 96857.06 0.2 492678.56 0 MItAJTISSIMA 0.0 0.0 0.0 0.0 98535.69 98535.69 0.1 492678.56 0 ACHt4AtITHES (LPIL) 290201.44 0.0 63895.81 0.0 70819.44 56223.91 0.1 0.0 0 RHOICOSPHENIA 0.0 0.0 37370.53 0.0 7474.11 7474.11 0.0 ,

g s 0.0

$ 0 CURVATA 0.0 0.0 37370.53 0.0 7474.11 7474.11 0.0 0.0 0 #4AVICULALES 0 11AVICULA 0.0 33288.75 37370.53 33055.06 31691.28 8866.62 0.0 54742.06 0 #4AVIC'lLA E LPIL) 0.0 33288.75 37370.53 33055.06 31691.28 8866.62 0.0 547*2.06 0 GOT1PHOttEMA 0.0 0.0 261593.69 0.0 107474.06 65852.31 0.1 g 275776.75 0

g 0 OLIVACEUn 0.0 0.0 261593.69 0.0 85163.94 54376.92 0.1 3 164226.19 '

O O O GOMFHONEllA (LPIL) 0.0 0.0 0.0 0.0 22310.11 22310.11 0.0 g 111550.56 k0 0 0

BACILLARIALES i41TZCHIA 0.0 0.0 17202.72 0.0 3440.54 3440.54 0.0 0 0.0 m

& 0 HITZCHI A (LPIL) 0.0 0.0 17202.72 0.0 3440.54 3440.54 0.0 1 0.0.

.G 0

3 l

. .. a

Table 2-7 (Contd)

DENSITY IN NUMBCR / SQUARE CENTIt1ETER STAT'Dtl REPC;tT Mean LS TAXA Lake Michigan Stations -

REL X S.E. ABX 1 10 11 12 25 0 DACILLARIDPHTTA-PD04 ATE ( LPILI 322446.06 59130.69 73725.94 53350.84 103829.37 53754.23 0.1 35493.34 i

TOTAL 297J65904. 32063792.0 7722576.00 66049392.0 90699008.0 52500736.0 100.0 50573228.0 DIVERSITY (H FEIMEI 1.00 1.11 1.71 0.20 0.87 0.27 0.34 DIVERSITY (J FRIt:El 0.48 0.52 0.59 0.10 0.37 0.10 0.15 m tRJMCER OF TAXA 9 7 12 6 22 AEDVE CCt:PUTED USIt;3 SAMPLE IDS 11 12 101 102 111 112 121 122 251 252 e

0 0

3 0

e e

S 3

E 4

e -

f.

o 3

Table 2-7 (contd)

DENSITY IN hut 18ER / SQUARE CENTIttETER STATICt1 REPCRT g

Nearshore Ponds -

REL ,

LS TAXA X S.E. AS:C 17 19 21 0 utIDENTIFIED ALGAE 576.70 1253.22 0.0 609.97 362.15 0.4 0 UNIDEt4TIFIED ALGAE ILPIll 576.70 1253.22 0.0 609.97 362.15 0.4 0 CYAf4GPHYTA 0.0 37758.06 0.0 125E6.02 12536.02 7.5 0 OSCILLATORIACEAE O Lit:28YA 0.0 37758.06 0.0 12526.C2 12586.02 7.5 0 L)HOETA (LPILI 0.0 37758.06 0.0 12566.02 12526.02 7.5 0 CHLOROPHYTA 353S2.68 28790.54 142442.31 68371.81 36834.42 40.8 0 CHLCRCCCCCALES 0 SCEr.EDES1US 20411.37 7551.62 9320.99 0.0 5958.30 5.5 0 ACUTUS 0.0 7551.62 2517.21 2517.21 0.0 1.5 0 GUADRICAUDA 20411.37 0.0 0.0 6803.79 6803.79 4.0 0 CHARACIUN 0.0 0.0 88731.44 29577.14 29577.14 17.5 0 At:LIGUUN 0.0 88731.44 29577.14 0

0.0 29577.14 17.5 CHAETOPHORALES O CHAETOFHORALES "C" 0.0 0.0 46253.66 15417.88 15417.88 0

9.1 g CHAETOl'HORALES "C" (LPIL) 0.0 0.0 46253.65 15417.88 15417.83 0

9.1 a OE00CCt&IALES y 0 OEDCGOHIt 1 12903.39 21238.92 7457.22 13066.51 4007.47 8.2 0 CEDOG341Ut1 ( LPIL I 12903.39 21233.92 7457.22 13S66.51 4007.47 8.2 0 Z1GhEti ATALES 0 CCENARItAt 1033.96 0.0 0.0 344.65 344.65 0.2 0 C00ttARIUt1 ( LPIL i 1C33.96 0.0 0.0 344.65 344.65 0.2 0 STAUPASTPUr1 1033.96 0.0 0.0 344.65 344.65 0.2 0 STAURASTRuti (LPIL) 1033.96 0.0 0.0 344.65 3 W.65 0.2 0 BACILLARIOPHYTA-CENTRIC 5190.31 0.0 0.0 1730.10 1730.10 1.0 0 EUPC31 SCALES 0 EUP001 SCALES ILPIL) 5190.31 0.0 0.0 1730.10 1730.10 1.0 0 BACILLARIOPHYTA-PErc: ATE 145615.12 9752.82 99516.44 84962.44 0 FRAGILARIALES 39090.30 50.3 g

g 0 DIATC:1A 8271.68 0.0 943.95 3071.83 2614.14 0

1.8

{ VULGARE 0.0 0.0 943.95 314.65 314.65 0.2 3 0 TENUE 8271.68 0.0 0.0 2757.23 2757.23 1.6 0 0 SYtC39A 24504.87 0.0 0.0 6168.29 0 8163.29 4.8 0 Sit 4EDRA (LPILI 24504.87 0.0 0.0 8163.29 8168.29 4.8 5 0 EUt10TI AL ES S 0 EUNOTIA 1033.96 0.0 0.0 344.65 344.65 0.2 2 0 PECTIHALIS 1033.96 0.0 0.0 344.65 344.65 0.2

{ 0 ACHtaANTHALES

$ 0 ACKHANTHES 106924.12 9439.52 6243.68 41537.44 33705.93 24.6 8 0 LAt4CEOLATA 0.0 0.0 5663.71 g 1887.93 13S7.93 1.1 0 ti!!aJTISSIMA 105463.9% 9439.52 '33301.15 0.0 33691.77 22.7 7 0 ACHNANTHES ELPILI 3460.21 0.0 584.97 1348.39 1069.32 0.8 9

I 3

k. ._)'

Table 2-7 (Contd) o CENSITY IN PAR'3EE / SGUARE CENTIt1ETER

TATIOta REPORT Mean

!!earshore Ponds - SEL LS TAXA X S.E. ABX 17 19 21 0 NAVICULALES 0 Gottett08:EttA 0.0 0.0 2943.85 952.95 002.45 0.6 0 OLIVACEUt1 0.0 0.0 Ie 31.H 543.95 c'3.25 G.6 0 GCr.FetOt41t'.A I LPIL) 0.0 0.0 116.59 35.00 39. C 3 0.0 0 EPITHEllIALES 0 EFITHEMIA 0.0 0.0 1946.40 e43.60 (4S.20 0.4 0 T!DGIDA 0.3 0.0 1946..C 64 3.6C 645.53 0.4 0 2ACILLARIALES 0 t:ITZCHIA 1730.10 0.0 0.0 576.70 576.7G 0.3 0 141TZCHIA (LPIL S 1730.10 0.0 0.0 576.73 574.70 c.3 0 BACILLARIOF;irTA-pet 24 ATE iLPIll 1153.40 313.30 87428.56 9531.75 28390 40 17.6 TOTAL le676T.60 77554.50  :*1956.62 163740.05 483c5.80 1C0.0 DIVERSITY (H FRIMCb 0.C9 1.;0 1.76 1.69 0.0F OIVER311Y IJ F21tfE) 0.73 0.79 0.71 0.7% 0.00 ta.1CER 07 TAXA 13 6 10 0

< N ASOVE CCP.PUTED U3!!:3 SAttoLE 105 M 171 172 191 19:

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lr co e.

8 0 m a no

• a e4

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H .r m .a .s en ee <> M +

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=

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w r

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3 8

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w &

.A G. e4 Z -s M od O MM M M w

h8

< M

= O w

'8 W

• a .A.

. .w n g:-

u sz - E - w< < u << <

< <D E < ., s s -

< ~~ ~

r > a = 8 > n o 8 > 8 mr I ti=4 $.,. a u w = u seu.

a < u z

o.e X

w

<2~

e E

E O

5 u u>

s 0

E C

u.

e

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N w < z.

e -O' O I e4 4 $ u >e e4 -

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2 34 solence servlees division 1

Table 2-8 (Contd)

O n

610VOLUrtE Its t11CROLITERS / SQUARE CENTIMETER STATICtl REPGRT

, Mean t.ake Michigan Stations -

REL g3 7. x- S.E. ABT g 1 70 11 12

' 25 0 02CILLARIOPHTTA-FEt34 ATE ( LPIL) 0.05 0.02 0.02 0.02 0.02 0.01 0.3 6 0.01 I

TOTAL 29.83 2.52 3.78 4.44 8.37 5.39 100.0 1.29 DIVERSITY (H PRINE) 0.83 0.80 1.71 0.43 1.13 0.29 1.91 DIVERSITY (J PR!Nf3 0.40 0.33 0.59 0.25 0.49 0.10 0.84 NU:1SER OF TAXA 9- 7 12 6 22 8

y' ABOVE COMFUTED USIllG SAf1PLE IDS 11 ta 12 101 102 D 111 112 121 122 251 252 e

O G

3 0

0 e

9 d

E S

e IL 1

0 3 .

4

44

, j

.J )g o. O. O. - o. o. N. N. o. e. 4 4 in. M. 4A. 4A. N. N. in. M. P*. d. N. o. M. M. o. O. O. 08%

. 4. M. -

Wm OOO Oo4 OoOMM Oo MH OooOo oN 4 N f4 4 4 HH 4eMO 3 at b Nb N ooo ao4 Ooooo oa 44 oaoao oN O o a rt e.0 oo oooo and o. o. O. o. a. o. o. o. O. O. O. O. o. D. O. o. o. o. a. o. o. o. U. o. O. O. O. o. O. o. u. o. o.

• Ooo Ooc oDooO oO oo OOOOo oo OoOoO oo OooO M

C ooo coo ocooO oo onoon OM eeooHH co ee o n o m .es

s. o.

o e. n. o. c. o. o. O. o. o. o. o. o. o. o. o. O. o. O. o. O.

c. o. O. o. o. n. O. O. o. o. u.

e >: Ooo o ss o Oocoa ve o ao ooooo oo GooCo OO OooO e e4 oo e4 in ww oo e

o. Cs. .Q O. O. o. a. o. o. O. O. O. o. o. o. O. o. o. o. o. o. o. o. o. a. o. O. O. o. o. o. o. O.

HOOO ooO Ooooo oO Oo oOoOO oO OOOoO oo ooOo N

D

^ C 9 O M O.

C U

O W oOn one ao de o o o s w o O. o. o. o. o. o. O. O. O. o. o. o. o. a. o. o. o. o. o. o. o. c. o. c. o. o. O. o. o. c. o. O. o. I r ch ooo oOo Ooooo oO oo ocooO oo OOOOo oO oOOO G3 m d 0 L N 9 GJ

@ 2:

eA

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o. o. o. o. O. . o. o. o. o. o. a. o. .. o. O. o. C. o. a. o. D. o. o. o. o. a. o. O. O. o. D.

NOOo OoO orooO oO oo oaooo ao ODOoo DO ocoO M

E.#

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k W

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U

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% 0. 3 ! aM >

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J -

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.J $ a gg ww w =

M J &Z MN CL w M

n.

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.J o.J 4 u CL M.4 t a w at =t w E C .e( .e (i D. H >=e.

o at < < 4 d aC A m fr IA " E 10 3 3 >= M En D- 6.nA =J J M fo af in W w w

• u- nuow m n z -, 5- .2 w.Js ** 5 a--a w e a.sz w > ,e w

• w w .4 n w 5

on ww 3 4 ar < u c~s oc.a< <--no < < a.

u w o =Jzo-z

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c > a. w< uuo g g :

~

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=E- ; 5 3. n: -~ c~ u y K >o.

2 g M- 4y - 8 w= u u .J M $

w N

ce

=eC G;t

>4 .H J

> ~>.o w Ec tu u n.4 at I er

<<ku o. w.* w 5 o 4 o o a w W H as as < a w z Z o 68 w Z o o W 8 J u

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m e- e ooOooOoooooooooooooooooooooooooooooooooooo j

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I pg SOISMOS SerVIOGS dOVOSIOR k

h Table 2-8 (Contd)

BIOVOLUME Itlt1ICROLITERS / SQUARE CENTIttETER STATIO!1 REPORT gggg Nearshore Ponds -

REL LS TAXA X 5.E. ABX

• 17 19 21 0 NAVICULALES S GOf'.PHCt!E!1A 0.0 0.0 0.00 0.00 0.00 0.3 0 OLIVACEUN 0.0 0.0 0.00 0.00 0.00 0.3 0 GO!1PHOMEtu (LPIL) 0.0 0.0 0.00 0.00 0.00 0.0 0 EPITifEMIALES 0 EPITHEMIA 0.0 0.0 0.03 0.01 0.01 7.0 0 TUPGIDA 0.0 0.0 0.03 0.01 0.01 7.0 O BACILLARIALES 0 NITICHIA 0.00 0.0 0.0 0.00 0.00 0.1 0 t11TZCHIA (LPIL) 0.00 0.0 0.0 0.00 0.00 0.1 0 BACILLARIOPHYTA-PEHt1 ATE (LPIL) 0.00 0.00 0.01 0.00 0.00 1.9 TOTAL 0.22 0.06 0.13 0.14 0.05 100.0 DIVEPSITY (H PRIME) 1.71 0.54 1.33 1.20 0.34 DIVERSITY (J PRIf1E ) 0.59 0.51 0.50 0.53 0.03 tiut10ER OF TAXA 13 6 10 22 N

y ABOVE C0:1PUTED USIttG oat 1PLE 10S 171 172 191 192 211 212 D

i o

e e

8 2

e e

0 Ne 0

3

@A total of 22 taxa were identified from both the Lake Michigan samples and the nearshore pond samples, a decrease from the D and 38 taxa observed in April from Lake Michigan and the nearshore ponds respectively. Both diver-sity (H') and evenness (J') indices were higher in the nearshore ponds be-cause of the greater number of dominant taxa (i.e., more even distribution of dominant taxa).

Periphyton samples collected as scrapings from natural substrate (Lake Mich-igan) or glass-slide substrates (nearshore ponds) were also analyzed for dia-com relative abundance (Table 2-9). As shown, the eight stations yielded a total of 82 taxa. As shown; e Centric diatoms were most comnon at Station 10.

e Fragilaria vaucherine was found in high numbers only at nonplume stations.

e Nitzschia fonticola was most common at Station 10.

e Achnanthes spp., Anomoeneis vitrea, Epithemia turgida, Eunotia spp., and Pinnularia viridis were common only in the nearshore ponds. ,

J Periphyton diatom composition in June 1978 was slightly more diverse than that of June 1975, 1976, and 1977. However, th. common (i.e., dominant) taxa from the lake and pond stations were identical with those of previous years. The system, as measured by this parameter, appears to be stable.

2.2.3.2 Chlorophyll a_. Periphyton chlorophyll a concentrations (micro- -

liters / square centimeter) '~or June 1978 are shown in Table 2-10. Higher val-ues were recorded at the Lake Michigan stations, which had long-term exposure, -

than in the ponds, which were exposed for only one month. Values bore no dis-l

! cernible direct relationship to density (Table 2-7) but did bear a relationship l

to the biovolume (Table 2-8), higher biovolumes generally corresponding with l

( higher chlorophyll a_ concentrations.

Chlorophyll a_ concentrations in June 1978 were comparable to those of the pre-vious year, an indication of the continuity in the study area from year to year.

)

2-38 **'*"****"'***dk*" ,

P' O

Table 2-9 Relative Abundance of Periphyton Diatoms at 8 Stations, Bailly Study Area, June 1978 Station and Pepitcate 11 12 17 19 21 25 1 10 b a b a b a b 4* b a b a b Dis tor 4 a b a Centric cyclotella comts 0.5 C. butzTEivina

• 0.5 0.5 niniana 1.0

[.meneilasp.

CycT3G 2.0 3.0 1.0 met iTri iranulata 0.5 GTiddica 2.5 1.0 2.0 1.0 2.5 1.5 2.0 3.0 1.0 5.5 M. Italica 3.0 3.0 41ostra sp. 1.5 2.0 1.0 5ternanodiscus astraea 4.0 9.5 16.5 19.0 0.5 3.0 2.0 2.0 '

15.5 0.5 F.5 2.0 0.5

5. eentzs *TT- 2.5 1.5 fte F od'li U s sp. 4.5 12.5 2.0 vennate .5 4 knanthes etijga 1 'anceolate 62.0 27.5 15.5 I TTGarts 17.0 13.0 K. einutisitma 0.5 1.0 4.5 0.5 3.5 77.0 82.0 33.3 41.5 IcnnTatnes sp. 5.0 0.5 KitTso'2icTosc normanti 0.5 1.0 1 nipieura sp. 0.5

-hora ovalis 0.5

- omoTa e rs7frea 4.0 13.3 0.5 0.5 0.5 0.5 KiierroGT1a r cemosa 3.0 (5cc&iiT~pTecentula 0.5 (k conets sp. 2.0 tTTiura l~ solea 0.5 0.5

@(g_telTe i77tnis 14.5 2.5 0.5 3.0 C. miTta 0.5 C. pEsEsta 0.5 0.5 0.5 Cymrella sp. 1.0 Tist W tenue 8.5 17.0 3.0 28.0 74.0 75.5 17.0 11.5 1.0 26.7 3.5 3.5 39.5 74.5

5. vu12are 1.0 0.5 1.5 2.0 T&eli~sp. 0.5 1.0 0.5 itneaia turgida 17.0 45.0 unotia f f E=uosa 0.5 E. pectin 41is 2.5 2.0 0.5 1.0 functis so. 14.5 3.5 6.0 Fragilaria crotonensis 4.5 3.0 F. pinnata 0.5 1.0 1.0 31.5 29.5 5.5 10.0 14.5 56.5 59.5 1.0 3.0 12.5 1.5 1.0 7.5 7.0

f. vaucneriae

f. s ecie7' 2.5 frq araif 15.5 4.0 7.0 1.0 2.0 F rustuiTTrnmecides 0.5 13.3 1.0 14 3 7.0 0.5 1.5 2.0 2.0 2.5 20.5 2.0 carononema olivaceum G. parvulum 0.5 Corr.noren ip. 0.5 3.0 2.0 1.0 2.0 51rosic.a sp. 0.5 1.0 Cavicula eit99a 0.5 1.0 N. Sali, arum 0.5 1.5

4. 'subnamu lata 0.5

4. tripunctata 3.0 1.0 2.0 Ta Ccuia so. 2.5 3.0 4.0 0.5 3.0 4.0 0.5 iTt scola amphibia 0.5 L angustata 1.0

5. dissipata 0.5 12.5 1.0 1.0 1.0 0.5 0.5

4. fonticola 2.5 9.5 11.0 2.5 1.0

5. hteiniTina 0.5 9.0 0.5 R. pelee 0.5 0.5 0. 5 '

Tittschte so. 1.0 1.5 2.5 1.5 2.0 2.5 13.3 2.5 Pienularia virfois 1.0 annuospnenta curvata 3.0 1.0 1.0 1.0 Dhopaloo Q a 0.5 1.0 Surir*11e ae92stata 0.5 0.5 v.5 1.0

5. ovat T  !.5 f metr Toelteattsstra 4.5 5.0 5'.74 2.0 0.5 3.0 L iTe'Etes d* 0.5 i nnetra sp. ~ 0.5 1.5 0.5 1.0 1.0 N.TTiria fenestrata 4.0 2.5 5.5 3.5 c.5 2.0 2.0 6.0 1.0 0.5 2.5 1.0 T. ficcculosa 2.0 Catd. Upcoticates 1.0 4.5 2.0 0.5 1.5 1.0 1.0 Unid. Frag 11artaceae Unis. 'taviculates 1.0 0.5 0.5 1.0 1.ntd. pennales 0.5 2.f 7erceatages based on less twan 200 organisms as diatoes were uncorren in the sample.

2-39 science servloes division

@ Table 2-10 Periphyton Chlorophyll a, Concentrations (pi/cm 2) Recorded from Periphyton Samples, Bailly Study Area, June 1978 Station Replicate (a) Replicate (b) X 1 4.957 0 2.479 10 1.434 0.592 1.013 11 0.645 0.233 0.439 12 1.381 5.298 3.340 25 0.466 1.264 0.865 17 0.245 0.051 0.148 19 0.020 0.020 0.020 21 0.108 0.072 0.090 AllLake(1,10,11,12,25) 1.627 All Pond (17,19,21) 0.516

)

2.3 ZOOPLANKTON 2.3.1 METHODOLOGY. Zooplankton samples were collected in Lake Michigan and the nearshore interdunal ponds in April and June 1978. At each lake sta-tion (1 through 10) four samples were collected by bottom-to-surface vertical tows of an 80-micron mesh, 0.5-meter diameter plankton net. Each sample com-prised a composite of four such tows condensed into one 1-liter sample. Addi-tional zooplankton samples were collected at the surface in the nearshore in- .

terdunal ponds (stations 17 through 21) with a 6-liter Van Dorn bottle. Each of the four replicate samples was concentrated to 1 liter. All samples w9re fixed to a final concentration of 4 percent buffered formalin.

Each sample, after being prepared with 5 milliliters of Rose-Bengal dye, was analyzed for quantity and composition of all mature and immature zooplankters (excluding nauplii, rotifers, and protozoans) with dimensions greater than 80-micron mesh aperture of the plankton net.

J 2-40 science servlees division

1 2.3.2 RESULTS AND DISCUSSION. The numerical abundance (number per cubic meter) of each taxon collected in June 1978 is presented in Table 2-11 and Ap-pendix E.

A total of 25 taxa was collected in Lake Michigan during June 1978, equal to the number collected in June 1977. Lake Michigan densities averaged 7,616 or-ganisms per cubic meter in June 1978 as compared to 13,168 per cubic meter in June 1977. However, as in 1977, copepods dominated the densities (85.8 percent in 1977, 75.9 percent in 1978). Major components of the fauna included calanoid (19.6 percent) and cyclopoid (42.0 percent) copepodids and Cyclops bicuspidatus thomasi (12.9 percent).

Densities at the nearfield stations (7,723/ cubic meter) were approximately equal to those of the farfield stations (7,509/ cubic meter). Densities were highest at stations 1 and 2 and lowest at discharge station 10. No consistent east-to-west or onahore-to-offshore density pattern was observed however.

Densities in the nearshore ponds averaged 258,937 organisms / cubic meter, and a total of 40 taxa were collected. Densities were highest in Pond B, lowest in Cowles Bog, but all values were many-fold higher than Lake Michigan values.

Cladocerans dominated (79.0 percent) the pond fauna, as opposed to the lake where copepods dominated. The greatest numbers of organisms collected were Bos-minidae (42.2 percent) and Ceriodaphnia sp. (20.6 percent). The other dominant taxa observed was cyclopoid copepodids.

A comparison with June 1977 data is shown below.

1977 Density 1978 Density (No./cu. meter) (No./cu. meter)

Pond B 644,798 359,636 Pond C 666,666 311,687 Cowles Bog 9,416 105,438 Although Pond 3 and C densities were lower, Cowles Bog values were higher in 1978 than in 1977. However, a comparison of predominant taxa shows that the changes in density have not resulted in a concommitant change in taxon composi-tion.

2-41 solence servloes division

l l

i Table 2-11 Zooplankton Density, Bailly Study Area, June 1978 1

Stations Lake Michigan SITE StntARr N*d "

Hearfield Farfield ,gt LS TAXA l-6 & 10 7-9 x S.E. ASx 0 0 9.63 12.59 11.11 1.48 0.1 0 NEttA1CDA iTOTAL) 11.11 1.48 0.1 1 ftEttATCD A (LPIL) 9.63 12.59 7.97 2.33 5.15 2.82 0.1 0 OLIGOCHAETA (TOTAL) 0 tMIDIDAE 5.15 2.82 0.1 1 tu1DID AE (LPILI 7.97 2.33 2.59 0.0 1.30 1.30 0.0 0 ARACHil!D A _l1CTAt t 0 PROSTIGPt A T A 2.59 0.0 1.20 1.30 0.0 1 HtCRACARINA (LPIL) 1812.62 57.21 23.8 0 CLADCCERA (10 TALI le69.84 1755.41 0 EOsttitdID AE 1633.81 1714.96 1674.3S 40.57 22.0 1 eCSitItIDAE (LPILI 0 CulCCRIDAE 12.90 12.90 0.2 1 ALCHA EECTAtiSULA 25.80 0.0 1.29 0.0 0.64 0.64 0.0 1 ALO'4A iLPILi 112.50 83.29 1.5 1 Cit:Dorus (LPI.) 200.79 24.22 3.25 0.0 1.93 1.93 0.0 w 1 EUP CER2US LAttELLATUS 0 Orni!!IDag

[

n 1 DArtin! A At'SIGUA 1.30 1.96 1.63 0.33 0.0 1.43 11.05 6.*4 4.61 0.1 1 DirHill A PE1FCCtRVA 0.93 0.0 0.0 1.96 0.93 6 D?.DitIIA 4LPILI 0.14 CEPICOAFNNIA (LFIL) 1.56 1.27 1.41 0.0 1

0 COPErCOA (10TAL D 5S27.52 5739.29 5783.40 44.11 75.9 0 CAtanDIDA iTOTAL)

• 5.06 46.30 35.6S 10.62 0.5 1 DIAPICtilS ASHLAtcI 7.66 1.49 0.1 1 DIAFict'US ttitRJTUS 9.17 6.19 Et91TEtCRA AFFINIS 16.42 0.0 8.21 8.21 0.1 1

1.09 29.50 15.29 14.20 0.2 1 lit 10Cf L AWS ttACEURUS 0.23 10.C0 5.12 23 0.1 1 EPISCHU7A LJCUSTRIS 1143.09 1838.66 1440.87 347.79 19.6 1e Calif:010A (trILI 8 0 C'CLCf DID A (TOTALI O 681.79 1287.86 ce4.83 303.03 12.9 1 C CLCTS BICUSPID ATUS THOttASI 0 CICLDr5 VEPitALIS 30.11 23.11 26.61 3.50 0.3 1

0.78 0.78 le 1 1

EL'C)CLCPS GrfpATUS TFDr001 CLOPS FR AS!ttfS ttEXICAHA 1.56 1.56 0.0 0.0 0.7S 3202.50 0.78 0.0 0.0 7C6.10 ,42.0 i 14 CIClor01DA 4trILI 3908.60 2496.40 g 0 HAFPACTICOIDA (10fALI ,

4.20 2.10 2.10 0.0 i

)g 14 1

H ARP ACTIColD A ( LPIL)

H/RPACTICOID A ( LPIL D 4.65 0.0 1.27 2.96 1.69 0.0 0 *DIPTEPA i:Et?ATCCERA ITOTAL) 5.14 0.0 2.57 2.57 0.0 g

3 0 CHIPCe f0f TID AE 2 CHIRCtCIIDAE (LPIL) 5.14 0.0 2.57 2.57 0.0 g

TOTAL 7722.69 7509.62 7616.15 106.53 100.0 8 1.93 1.99 -1.96 0.03 DIVERSIT( (H DTit1E l I DIVERSITT EJ FrittEl C.66 0.70 0.68 0.02 3 145T.ER Cr TAXA 24 17 25 L Q,- A

Table 2-11 (Contd)

O SITE StaniART Nearshore Ponds Hean u 7,x, Pond B Pond C Cowles Bog  ; 3,g, $$

1 1 1 0 CHIDARI A ( TOTAll 13C".89 0.1 0.0 462.96 462.95 0.2 0 HECROZOL 19 41)CRt (LPILI 1388.89 0.0 0.0 460.96 462.96 0.2 0 Hft1&T00A (1% tLI 694.44 148.81 1544.12 795.79 405.97 0.3 1 I:Etti.1CD A I LPIL 4 694.44 148.81 1544.12 795.79 405.97 0.3 0 OLIGOCliAEla iT37AL) 173.61 148.81 2059.50 793.97 632.30 0.3 0 HtIDIDAE 1 Cil AE100 AS T E R (LPIL) 173.61 0.0 0.0 57.87 57.87 0.0 1 NAIDIDAE (LPILI 0.0 148.81 2059.50 736.10 663.09 0.3 0 ARACilNICA ETOTAL) 520.83 173.61 381.94 358.00 100.90 0.1 0 PEOSTICitt14 19 H)CRAC/RIHA (LPILI 500.83 173.61 381.94 358.80 100.90 0.1 0 CL ADOCEP A (TOTAll 327013.44 039992.69 46781.68 004505.37 82609.31 79.0 0 COSilifilDAE 1 00S!!It!!D9E e LPIL) 143090.12 170141.69 14503.*1 109:51.69 48003.61 42.2 0 Cll DCRIDAE 1 ALO:tA EECTAtGULA 6319.44 7109.12 008.33 4545.63 2180.60 1.8 AL0tlA ATFillIS 0.0 206.35 206.35 0.1 Y

e 1 0.0 619.05 0.0 303.5 003.77 116.76 0.1 1 ALO!n CU'.DRAtTULARIS 277.78 W 1 CAtiPICCErCUS RECTIROSTRIS 500.C3 6027.77 0.0 21e0.87 1908.3: 0.8 1 Cil)DORtiS ( trIL) 29070.80 2c003.95 9:5.04 16733.33 03'+4.46 6.5 1 EU7ZIA LATISSIr1A 0.0 14S.81 2506.55 891.79 818.51 0.3 1 GRAr10LECERIS TESTUD!HARIA 0.0 140.06 0.0 414.02 414.00 0.2 1 LE1DIGIA OU'.0RuGtfLARIS 0.0 119.05 0.0 39.68 39.68 0.0 1 OX t URE L L A TEt191C Al")15 0.0 0.0 115.74 38.58 3S.53 0.0 1 FLEUROSUS DENTICULATUS 3784.72 9796.62 4787.57 6122.97 1859.50 2.4 1 PLEty? cars FRectrWUS 173.61 426.59 6147.87 0:49.35 1950.62 0.9 0 DATHMDAE 1 Dir;ttilt At:81004 008.33 0.0 0.0 69.44 69.44 0.0 1 DariftlI A RETROCL"JVA 555.56 0.0 094.12 083.20 160.47 0.1 e 1 EItCCErilALUS I LPIL) 0.0 946.43 5954.52 2300.31 1847.41 0.9 0 1 CEPIDDAfFHIA (LPILI 136944.05 12360.11 10909.01 53403.05 41772.71 20.6 0 0 t13CF01HRICIDAE 3 1 ILYORtPTUS SP1HIFER 0.0 563.49 0.0 187.83 187.83 0.1 l 1 0

r! ACRO 11!RIX ROSCA SIDIDAE 0.0 2649.80 0.0 883.27 833.07 0.3 O I DI ArliatOScttA (LPIL) Se68.05 7838.29 0.0 4568.78 2354.13 1.8 0 OSTRACOD A (10i AL ) 1 15.28 3S71.03 0322.98 2469.76 770.15 1.0 19 OSTPAC004 (LrILi 1215.28 3371.03 03:2.93 0469.76 770.15 1.0 0 0 Corer 0DA 4TOTAll 069 % .41 58076.72 45454.75 43555.63 9094.41 16.8 l 0 1

CAtti'3IDA I TOTAL i DIAPTDt".JS PALLIOUS 6C05.55 0648.81 0.0 3151.45 1980.60 1.2 b 1 EtTtiltrRA AFF1HIS 555.56 0.0 0.0 185.19 185.19 0.1

f. 1 lit:'OCALANUS ttACFURUS 173.61 0.0 0.0 57.87 57.87 0.0 e .

I 3

- _ _ _ _ _ _ _ _ - _ _ _ - - -=. .

Table 2-11 (Contd)

O SliE SutruRr Nearshore Ponds Mean

- REL (S TAXA Pond B Pond C Cowles Bog x S.E. A8x 1 1 1 14 CAL #toIDA ILPILI 8958.32 357.14 115.74 3143.74 2908.13 1.2 0 C)CLCr01D4 iTOTALi 1 C) CLOPS BICUSPIDATUS THOrtASI 1:50.00 077.78 243.06 590.28 330.01 0.2 1 CYClors vtRNALIS 1333.89 0.0 1875.00 1087.96 561.79 0.4 1 EUC) CLOPS AGILIS 347.22 6273.80 3523.:S 3381.43 1712.33 1.3 1 ft/CROCtCL0rS AL810US 173.61 148,81 891.20 404.54 043.44 0.2 1 NE50 CYCLOPS ED AX 173.61 P.0 0.0 57.87 57.87 0.0 1 ItE50C) CLOPS LELNARTI 0.0 0.0 98.04 32.68 32.68 0.0 14 C)CLor0!DA ILPILI 7118.05 48396.78 37794.77 31103.20 12376.94 12.0 0 HARrACTICOIDA (10TAL) 14 HARTACTICOIDA (LPIL) 0.0 0.0 104.17 34.72 34.72 0.0 1 HARPACTICOIDA (LPIL) 0.0 173.61 809.50 3 7.70 046.Cb 0.1 O ErHEttEROPIER A 4 TOTALi 173.61 1718.25 0.0 630.60 5 6.12 0.2 0 CAEHIDAE 13 CAENIDAE ILPIL) 0.0 83.33 0.0 27.78 27.78 0.0 13 ErHEttER0PTER A ( LPILI 173.61 1634.92 0.0 602.04 518.47 0.2 0 CIPTERA HEtt*.TCCERA 4 TOTAll 1562.50 7358.12 6894.05 5071.56 1859.36 2.0 0 CHIRCt.UtIDAE '

N CHIPCMortIDAE iLPILl 1560.50 7358.12 6894.05 5271.56 1E59.36 2.0

• ~

• • TOTAL 3596e6.87 311687.87 10543S.81 258937.81 77990.25 100.0 DIVERSITY (H PRIMEl 2.05 2.43 3.03 0.50 0.28 DIVERSITY (J FPIttEl 0.56 0.60 0.73 0.64 0.05 NUICER OF TAXA 28 29 24 40 ABOVE CottrUTED USItG SAttPLE IDS 171 172 173 174 181 182 183 184 191 192 193 194 201 202 203 204 211 212 213 214 0

0 e

3 0

e e

e i 3 ii' e

e IL e-0 3

( (.: O

Replicate variability (standard error i mean) was calculated for all June sam-ples. Cassie (1963) has stated that a range of expected variability for net samples is 22 to 44 percent; no range was provided for Van Dorn samples. The observed range of values for June 1978 is shown below:

Station Replicate Variability (%)

1 18.9 2 26.8 3 18.5 4 18.7 5 9.4 6 12.1

7 11.9 8 9.4 9 14.5 10 10.3 17 7.8 18 15.1 19 41.1 20 7.8 21 10.2 As shown, all values, even for the Van Dorn samples, fall within or below the 22-or 44-percent range, an indication that the sampling technique is accurately as-sessing the lake and pond densities.

2.4 BENTH0S 2.4.1 METHODOLOGY. Ten Lake Michigan (1-10) and five nearshore pond stations (17-21) were sampled for benthic organisms. Duplicate samples at the lake stations were collected with a 9- x 9-inch Ponar sampler, while pond samples were collected using a 9- x 9-inch Ekman sampler. (All samples were picked under a 10X magnifying lens.) Organisms were preserved in 10 percent buffered formalin, then identified to the lowest practical and positive taxo-nomic level. Results for April 1978 are presented in Table 2-12, with indi-vidual station tables presented in Appendix F. All densities are expressed in terms of number per square meter.

2-45 science servlees division

Table 2-12 Benthos Density, Bailly Study Area, June 1978 o bet:1 HIC titCROINVERTECRATE nut 1ERICAL ADUt:D AtlCE tt40/SQ til Stations SITE SurstARf Mean Nearfield Farfield ts tax 4 1-6 & 10 7-9 O

x S.E. E O

O HEttATCD4 iTOTAll 54.49 41.67 48.08 6.41 5.0 1 HEt1AICDA (LPILI 54.49 41.67 48.08 6.41 5.0 0 OLICOC'fAETA (TOTAL) 348.29 580.13 464.21 115.92 48.0 0 f t# IDID t.E 1 CHtETCS ASTER ( LPIL) 2.14 51.28 26.71 24.57 2.8 1 H*.!S ILPIL) 3.21 0.0 1.60 1.60 0.2 1 STTLARIA (LPIL) 2.14 3.21 2.67 0.53 0.3 1 HAIDIDAE ILPILI 11.75 16.03 13.89 2.14 1.4 0 TUDIFICIDiE 1 TUDIFICIDAE ( LPIll 329.06 509.61 419.34 90.28 43.4 0 HIRL' DINE A t Tot AL I 23.50 28.85 26.18 2.67 2.7 0 GLOSS!PHC'3IIDAE 1 HELC09ELLA STAGNALIS 3.21 3.21 3.21 0.00 0.3 6 HIRUDI!:EA ELPIL) LO.30 25.64 22.97 2.67 2.4 0 G851FCPCOA (TOTAll 0.0 6.41 3.21 3.21 0.3 g 0 Af flICOLID AE 3.21 3.21 0.3 g 1 O BIVALVIA ITOTAL)

Art'41 COLA I LPIL) 0.0 41.67 6.41 28.85 35.26 6.41 3.6 0 $rH?ERIIDAE 1 Sr!!AERILRI ( LPILI 5.34 25.64 15.49 10.15 1.6 1 PIS10ILt1 ( LPIL) 36.22 3.21 19.76 16.56 2.0 0 ARACH!IIDA (TOTAL) 3.21 0.0 1.60 1.60 0.2 0 FFOSTIGttATA 1 H)DRActRIHA (LPIL) 3.21 0.0 1.60 1.60 0.2 0 AttrHIFODA ITOTAL) 122.86 41.67 82.26 40.60 8.5 0 H'USTORIIDAE 1 FO'iforOREIA AFFINIS 122.86 41.67 82.26 40.60 8.5 0 DIPTER A I:EttATOCERA ITOTAL) 349.36 262.82 306.09 43.27 31.7 0 C EP AT00000tlID AE l 1.07 0.0 0.53 0.53 0.1 2 CERATOT000*tIDAE ILPIL)

O O

2 CHIRot1G!1IDAE CHIRCt:0!=JS I LPIL) 59.83 70.51 65.17 5.34 6.7 9 2 CR1rTCCHIRO*to:tVS ( LPIL I 80.13 125.00 102.56 22.44 10.6 3 2 CRIC010PL'S ( LFIL I 2.14 0.0 1.07 1.07 0.1 9 2 DICROTE!!DIPES ILPIL) 0.0 3.21 1.60 1.60 0.2 2 2 FOLYFEDILLCl I LPILI 6.41 16.03 11.22 4.81 1.2 2 FRCCLADIUS (LPILI 1.07 0.0 0.53 0.53 0.1

{

g 2 C0" lit:0!!EL'2A E LPILI 45.94 0.0 22.97 22.97 2.4 8 2 HART! ISCHI A ( LPIL) 135.6S 32.05 83.67 51.82 8.7 g 2 PHAEtt0F0ECTRA (LPIL) 1.07 0.0 0.53 0.53 0.1 6 PSECTFOCLADIUS (LPIll 7.48 3.21 5.34 2.14 0.6 4 4.27 12.G2 8.55 4.27 0.9

== 2 PARACL.*DOPELitA ( LPIL)

S 2 f*C':0DI At1ES A ( LPIL ) 3.21 0.0 1.60 1.60 0.2

,3 CHIRONottIDAE ILPIL) 1.07 0. I' O.53 0.53 0.1

(. ' d

Table 2-12 (Contd)

Etts! HIC tt?CROtthERitERATE tEJtERICAL AEUPDANCE (HO/sQ N1 snE su-ner Stations Nearfield Farfield Nan l[I ts Tu.s 1-6 & 10 7-9 x 3,t, O O TOTAL 993.37 990.35 966.85 - 23.50 1C0.0 OIvtRSIT Y IH rRittE l 1.57 2.05 1.21 0.04 DIVERSITY (J PPIME l 0.60 0.73 0.67 0.07 t?_.TCER CF T4X% OS 18 27 EDTiert Tire O APDVE C0t1PJTED USI'G sat *PL E IDS 11 12 21 02 31 3: 41 4 51 50 61 62 y 101 lo: 71 70 81 60 91 92 N

e 0

F 3

0 4

e a

e e

g e

0 ,

3 o

Table 2-12 (contd)

EEHiltIC t1ACPOIllVERTECRATE tRAtIRICAL ABLR4DAIJCE IN0/SQ til SITE Stat:1ARY Mean REL (S Tax 4 Pond B Pond C Cowies Bog x S.E. Asz 1 1 1 0 CtlIDARIA (TOTALI 33.65 0.0 0.0 11.22 11.22 0.3 0 HIDR 0ZOA 1 HVDPA ELPILD 33.65 0.0 0.0 11.22 11.22 0.3 0 net 1ERTIH4 iTOTAll 4.81 0.0 0.0 1.60 1.60 0.0 1 NEttERTIf1A ELPIL) 4.81 0.0 0.0 1.60 1.60 0.0 0 tif t1 AT004 ( TOT All 0.0 19.23 250.00 89.74 80.32 2.7 1 I!EttATCDA (LPIL) 0.0 19.23 250.00 89.74 80.32 2.7 0 OLIGCCHAETA (TOTAll 552.88 1230.77 634.61 806.09 213.65 24.0 0 14P.IDID AE 1 CHAETOGASTER (LPILI 14.42 43.27 9.62 22.44 10.51 0.7 1 flAIDIDAE (LPILI 461.54 10C6.54 134.62 560.90 279.25 16.7 0 TUDIFICIDAE 1 ftTIFICIDAE ILPIL) 76.92 100.96 490.38 222.76 133.99 6.6 0 GASTECPCDA ( TOTAL) 4.81 302.88 125.00 144.23 86.58 4.3 0 P18YSIDAE 1 Pil)SA ( LPILI 4.8.1 38.46 105.77 49.68 29.68 1.5 N O PLAl:ORDIDAE 1

00 1 G)RAULUS ( LPIL) 0.0 81.73 19.23 33.65 24.67 1.0 1 HELISCitA ILPIL) 0.0 182.69 0.0 60.90 60.90 1.8 0 DIVALVI A (TOT AL I 14.42 370.19 3182.69 1189.10 1002.07 35.5 n SPHAERIIDAE I SrilrERIlri i LPIL i 4.81 370.19 346.15 240.38 117.99 7.2 1 PISIDIt'If I LPILl 0.0 0.0 2836.54 945.51 945.51 28.2 6 BIVALVI A (LPIL) 9.62 0.0 0.0 3.21 3.21 0.1 0 ARACll:11DA ITOTAll 0.0 9.62 0.0 3.21 3.21 0.1 0 PROSTIGil4TA 1 H)DRActR1tlA ELPIL) 0.0 9.62 0.0 3.21 3.21 0.1 0 EP1tEt:EROPTERA ITOTAL) 4.81 96.15 0.0 33.65 31.28 1.0 0 Ct Et4ID AE

1. 10 CAE!!IS (LPIL) 4.61 96,15 0 0.0 33.65 31.28 1.0 0 000?i4TA I TOTAL) 0.0 19.23 0.0 6.41 6.41 0.2 0 0 LIEELLULIDAE g 10 LEUCCRRilIllI A ( LPIL D 0.0 9.62 0.0 3.21 3.21 0.1 3 0 COLita 0RT0!IID A E g 10 CottlAGRIDt4ID AE (LPIL) 0.0 9.62 0.0 3.21 3.21 0.1 g .O COLEOPTERA ADErilAGA (TOTAL) 0.0 0.0 9.62 3.21 3.21 0.1

)

==

O 1

DYTISCID'E DrTICCID AE (LPIL) 0.0 0.0 9.62 3.21 3.21 0.1 g 0 DIPTERA I:Et1ATOCERA ITOTALI 817.31 2081.73 293.03 1065.70 529.66 31.8 3 0 CER410F000tlIDAE g 2 CERATOPC0CilIDAE (LPill 38.46 4.81 9.62 17.63 10.51 0.5

- 0 CHIRO: 3:110/i 1 2 CHIR 0!!C lUS ( LPILi 14.42 1875.00 153.85 601.09 598.31 20.3

!. 2 CRIPTCCllIRO!13t105 (LPIL) 14.42 0.0 0.0 4.81 4.81 0.1 0 .

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r 2-49 science sentoes ceivision

@2.4.2 RESULTS AND DISCUSSION. Within Lake Michigan (stations 1-10), mean density per square meter ranged from a low of 29 organisms at Station 10 to }

4,125 at Station 6, the latter station having also yielded the highest density in April 1978. Mean density for the lake stations was 966.9 organisms per square meter.

Dominant taxa included Tubificidae (43.7 percent of organisms collected), Ponto-poreia affinis (8.5 percent), Cryptochironomus sp. (10.6 percent), Chironomus sp. (6.7 percent), and Harnischia sp. (8.7 percent). Number of taxa during June ranged from a low of 1 at Station 4 to a high of 16 at Station 6, with a total of 27 collected over the entire Lake Michigan study area.

Benthic densities in the nearshore ponds ranged from a low of 826 per square meter at Station 19 to a high of 7,433 at Station 2, both within Pond C. Dom-inant taxa included Naididae (16.7 percent), Tubificidae (6.6 percent), Sphaerium sp. Pisidium sp., Chironomus sp., and Tanytarsus sp. A total of 31 taxa were collected; 21 in Pond B, 22 in Pond C, 15 in Cowles Bog. A number of these taxa were common to all three locations.

Pond densities increased from Pond B to Pond C to Cowles Bog, as they had in the j two previous sampling periods (November 1977 and April 1978); mean densities were 1,433, 4,130, and 4,500 per square meter, respectively. Density values in pre-vious June sampling perioda were equally variable. Pond B (stations 17 and 18) was dominated by Naididae and the chironomid genus Tanytarsus; Pond C (stations 19 and 20) benthic fauna was represented by Naididae, the gastropod (snail) Heli-soma, the bivalve (clam) Sphaerium, and the chrionomid Chironomus. The Cowles Bog samples were dominated by Nematoda, Tubificidae, and the clams Sphaerium and Pisidium. The distribution and occurrence of dominant taxa within the ponds were consistent with previous descriptions of the study area.

2.4.3 SEDIMENT PARTICLE SIZE ANALYSIS. Substrate sediment analysis was perfomed on regular benthic samples from Lake Michigan and the interdunal ponds during August 1978. Random subsamples were taken from each sample and strained through a National Bureau of Standards sieve series (No. 5, 10, 18, 35, 60, 120, and 230). The fractions passing through the No. 230 screen were caught in an enamel pan, dried at 110*C, weighed, and the percentage composi-tion calculated. Particle sizes were classified according to the Wentworth J scale as follows:

2-50 solence serv!ces division

l 1

l Table 2-13 Benthic Particle Size Analysis, Bailly Study Area, August 1978 )

>4 na 2-4 m 1-2 m 0.5-1 m 0.25-0.5 m 0.125-0.25 m 0.063-0.125 m <0.063 m Gravel Very Coarse Sand Coarse Sand Mediuri Sand Fine Sand Very Fine Sand Silt Clay Location Station NBS No. 5* NBS No. 10 NBS No. 15 NBS No. 35 NBS No. 60 NBS No. 160 NBS No. 230 NBS No. 230 Lake 1 0 0 0.02 0.12 77.77 13.57 0.23 8.28 2 0 0.02 0.69 7.54 81.44 8.55 0.18 1.5R 3 0.08 0.02 0.05 0 20.70 68.04 6.70 4.41 4 0.03 0.18 0.13 37.22 32.96 25.19 1.90 2.39 5 0 0.25 0.02 0 43.00 41.15 4.17 11.43 6 19.05 6.71 1.06 0.49 5.97 14.51 2.48 49.73 7 0.35 1.46 2.08 2.39 41.34 22.66 1.73 27.99 8 0.04 0.06 0.24 31.79 56.23 9.45 1.39 0.79 9 16.77 4.00 1.39 0.23 2.31 1.12 2.18 72.00 10 0.05 0.23 0.62 54.00 38.29 2.78 1.21 2.81 i Lake 3.64 1.29 0.63 13.38 40.00 20.70 2.22 18.14 g Shallow 1,4,7 0.13 0.55 0.74 13.24 50.69 20.47 1.29 12.89 h Mid-Lake 2.5,8 0.01 0.11 0.32 13.11 60.22 19.72 1.91 4.59 Deep Lake 3,6,9 11.97 3.58 0.83 0.24 9.66 27.89 3.79 42.05 Pond 17** 68.82 0.92 1.23 3.95 3.42 2.69 10.21 8.76 18** 10.89 0.32 2.66 6.15 17.95 33.43 0 28.59 19** 79.73 0 0 0 1.20 3.62 5.08 10.37 20** 63.24 0.05 0.35 1.01 7.30 7.06 5.23 15.77 21** 6.27 12.88 3.03 3.52 3.64 2.71 1.15 66.79 i Pond 45.79 2.83 1.45 2.93 6.70 9.90 4.33 26.06 o Naticnal Bureau of Standards Screen Size No. 5.

Sar.ples contained large amounts of organic material which collected in the No. 5 sieve. This riterial was not counted as part g of the sediment analysis.

S 4

2 i

9 I

e 0

3

Sediment Size (mm) Scale

>4 Cravel 2-4 Very coarse sand 1-2 Coarse sand 0.500-1 Medium sand 0.250-0.500 Fine sand 0.125-0.250 Very fine sand 0.063-0.125 Silt

<0.063 Clay Results of analyses for August 1978 are shown in Table 2-13 and comparisons with previous year's data are shown in Figure 2-2. As shown, values from station to station were quite variable. Ilowever, a comparison with 1977 data shows that, overall, particle size composition changed very little between August 1977 and August 1978.

2.5 AQUATIC MACROPlWTON 2.

5.1 INTRODUCTION

. One of the indicators of change in water quality within an aquatic ecosystem is a change in the aquatic plant community. Changes in both the micro (phytoplankton and periphyton) and macro (aquatic macrophyte) )

forms are observable. Considerably more information has been generated on en-vironmental tolerances of the microforms, but there also exists a growing data base on tolerances of the larger aquatic macrophytes. With this data base in mind, a study of the submerged and floating macrophytes was conducted in Pond B, Pond C, and Cowles Bog in the Bailly Study Area during 1978.

2.5.2 MET!!0DOLOGY. During the 1978 sampling, aquatic macrophytes were collected on June 19 at all pond sampling locations. Pond B samples were taken in the vicinity of stations 17 and 18, Pond C in the vicinity of stations 19 and 20, and Cowles Bog in the vicinity of station 21. At each of these locations, representative specimens were collected using a 9-inch by 9-inch dredge at five randomly selected points along a 250-foot transect. The transects were as close as possible to those of 1975 and 1976. Extent of coverage was estimated quali-tatively and quantitatively. Qualitative data were described in the following terms:

e Scattered individuals (or patches) e Uncommon (or relatively uncommon) 2-52 solence services division t

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1974 1975 1976 1977 1978 l

Figurt 2-2. Sediment Grain Size Distribution, Lake Michigan and l Interdunal Ponds, Bailly Study Area, 1974-1978 l

l 2-53 science services division r

l l

@ e Common (or common in certain areas) e Very common e Dominant Extent of coverage was estimated also in terms of grams dry weight per 81 square inches of sampler. Table 2-14 presents the results.

Table 2-14 Macrophyte Composition, Bailly Study Area, June 1978 Density Location Comon Name Scientific Noma Relative Abundance (gm/81 in.2),

Pond B Smartweed Polygonum pennsylvanicum Unconnon 3.5 Pickerel weed Pontederia cordata Scattered specimens 1.5 Pondweed Potamogeton natans Very comon 30.1 Watermilfo:1 Myriopnyllum sp. Dominant 60.5 Bullhead lily Nuphar sp. Dominant 45.0 Duckweed Lemna minor Common 0.02 Pond C Bladderwort Utric21 aria vulgaris Dominant 384.5 Bullhead Illy Nuphar sp. Dominant 270.7 Smartweed Polygonum pennsylvanicum Unconnon 3.5 Pickerel weed Pontederia cordata Scattered specimens 1.5 Duckweed Lemna a minor Common 0.07 )

Cowles Bog Watermilfoil Myriophyllum sp. Common 6.1 Ouckweed Lemna minor Very comon 1.0 Coar. tall Ceratophyllum diversum Common 5.0 Cattall Typha latifolia Dominant 60.0 Bladderwort Utricularia vulgaris Dominant 42.0 Bullhead lily Nuphar sp. Connon 5.0 Arrow arum Peltandra virginica Scattered specimens 0.6 2.5.3 RESULTS AND DISCUSSION. Summer 1978 macrophyte composition was similar to that of previous years. Some of the less common forms were missed because of the quantitative sampling technique employed in 1978. However, sam-pling yielded the same dominant and/or common species as in previous years -

bullhead lily (Nuphar sp.), smartweed (Polygonum sp.), bladderworr (Utricularia sp.), pendweed (Potamogeton sp.), and watermilfoil (Myriophyllum sp.) . As in previous years, the area in and around Cowles Bog was characterized by a pre-dominance of emergent species.

J 2-54 solence servlees division

O Diagrams of cnme of the common macrcphytes counted or seen within the ponds are shown in Figure 2-3 and a key to the common nearshore pond flora is pro-vided in Table 2-15.

\

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(1 p  %

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/

d i

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l i

Peltandra virginica (Arrow arum)

( \

l Pontoderia cordata (Pickerel weed)

Typha latifolia l (Ca tta t i)  ;

s Potamogeton natans (Pondweed) 3 d sy '

a

.k' ' Ceratophyllum demersum

,*,'[{1 ,

- b', > , (Coontail) qt,ke

$ ~

3 Brasenia schreberi (Water shield)

Figure 2-3. Some Common Macrophytes Found in Pond Areas in Vicinity of Bailly Study Area (after Hutchinson 1975)

Table 2-15 A Coneralized Key to the Common Nearshore Pond Macrophyte Flora Collected in the Bailly Study Area A. Free ficating, without roots or with roots pendant in water.

I. At surface, upper part of plant ordinarily dry.

Lemnaceae - Lemna minor (duckweed)

II. Below surface, plant entirely submerged, floating at mid-depths.

a. Leaves capillary with traps (utricularids)

Lentibulariaceae - Utricularia (bladderwort) s

b. Leaves capillary in whorls, without traps, roots absent but stems sometimes become buried (ceratophyllids). j Ceratophyllaceae - Ceratophyllum (Coontall)

B. Rooted in sediment (rhizophytes)

1. Part of vegetative structure emerging above water for most of year.

a. Elongate emergent stems with long cylindrical or narrow flat leaves.

Sparaganiaeae Sparganium (bur-reed)

Cyperaceae Caren (sedge)

Dulichtum arundinareum (3-way sedge)

Deocharis (spike rush)

Scirpus (bulrush)

Typhaceae hpha (cattail)

b. Leaf-bearing stem emerging well above water with air leaves that are usually lanceolate. .,

elliptical. or compound above water.

Polygonaceae Polygonum (smartweed)

]

Haloragaceae Proserpinaca (mermaid-weed)

c. Foliose. petiole extending above water 50 that the leaf rather than the whole shoot is emergent; flower stalk or inflorescence ordinarily emerges above water; emergent leaf cordate. sagittate, or lanceolate.

Pontederiaceae Pontederia cordata (pickerel weed)

Araceae Peltandra virginica (arrow arum)

II. Leaves, or at least some of them, floating but not usually emergent.

4. Floating leaves cordate. circular, or elongate-oblong.

Nymphaeaceae Nymphaea (water lily)

Nuphar (water lily)

Cabombaceae Brasenta (water-shield)

b. Floating leaves lanceolate Potamoge'.onaceae Potamogeton (pondweed)

!!!. Plant. except flower or inflorescence. submerged. perennially or during most of the growing season.

a. Vittate, long stems or creeping rhizomes with long flexible branches.

(1) Small leaves Hydrocharitaceae Elodea (waterweed)

(2) Leaves negriophyllord greatly divided Haloragidaceae Myriophyllum (milfoll)

'l

b. Stem very short, leaves in a rosette.

Hydrocharitaceae Vallisneria (eelgrass) 2-56 solence servloes division

O 2.6 FISHERIES 2.6.1 METHODOLOGY. During the summer quarter, 300-foot experimental gill nets having equal size panels of 1 to 3.5 'nch mesh (at 1/2-inch mesh intervals) were set at stations 4 and 7 (Figure 2-1). A 50-foot, 1/8-inch mesh seine was deployed at stations 23, 24, and 25, and a backpack shocker was used in Pond B.

The gear were deployed as described in the Standard Operating Procedures (TI 1974) and were uset in August 1978. Table 2-16 summarizes the catch for August.

Catches are expressed for the gill net in terms of individuals per 300-foot net per night sampling period, for the beach seine in number of individuals per haul per station, and for the electroshocker in number per.20-minute sampling period.

Ichthyoplankton (fish eggs and larvae) were sampled in Lake Michigan in the vi-cinity of the Bailly Site at stations 1-10 (Figure 2-1) by two methods: zoo-plankton net and epibenthic pump. The zooplankton net was employed at stations 1-10, with the ichthyoplankton collected as an adjunct to the zooplankton sam-pling. The epibenthic pump was used at stations 4 and 7; all of the demersal organicas in the vicinity of the pumping head were pumped up and into a 80-mi-cron mesh net suspended in the water column at the surface of the lake. Pumping velocity was high enough to minimize avoidance but low enough to minimize harm to the organisms.

2.6.2 RESULTS AND DISCUSSION. A total of 225 fish were collected (Table 2-16). Of these, 40 were salmonids (lake trout, brown trout, Chinook salmon, and steelhead trout), 44 yellow perch, 10 rainbow smelt, 1 gizzard shad, 1 channel catfish, 53 unidentified minnows, 73 alewife, and 3 black bullhead. The bullheads were collected in Pond B, while the remainder of the fish were collected in Lake Michigan samples. The salmonids were all collected in gill nets, as were all but one of the yellow perch. The channel catfish caught at Station 4 represents only the third specimen of this species collected to date. Two previous specimens were collected in 1975.

Condition factors for all fish were within normal ranges. Of the salmonids, the brown trout (Salmo trutta) had the highest condition factor, steelhead and lake trout the lowest. l l

No ectoparasites or lamprey scars (probable only for salmonids) were detected.

l 2-57 i.a.. ..rv i... ... . . !

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Table 2-16 Fisheries catch in Bailly Study Area, August 1978

- r.

Percentage Catch For Len9th weight Condition Station Date Comon Name Scientific Name (ne) We Factor Station Month Gear 4 8/21/78 Chinoon salmon Onj,ornynchu9 tschewy[sg 3 8 38 5902 1.003 1.5 2.2 Gill Net 9 30 7718 0.959 885 6810 0.982 880 7264 1.065 775 4994 1.073 Steelhead trout Salmo gatednert- 875 2724 0.886 6.0 1.8

~~~~

695 3178 0.947 776 3854 0.826 666 2724 0.922 troen trout 5almo trutta 622 3631 1.509 11.9 3.5

~

595 2497 1.185 574 2270 1.200 448 908 1.010 473 1135 1.012 349 567 1. 334 305 363 1.219 344 545 1.3J9 Lake trout Salvelinus na*aycush -

672 2124 0 898 16.4 4.9 114 4313 0.930 696 2951 0.875 650 2497 0.909 672 2497 0.823 655 2270 0.808 682 2951 0.930 687 2951 0.910 '

737 3859 0.964 695 2951 0.879 580 1589 0.884 Gif f ard shad Dorosome cepedianum 448 999 1.111 1.5 0.4 Ralebow smelt Osmerus mordas 157 17.5 0.452 3.0 0.9 132 11.5 0.500 Channel catfish j,Qalvrvspunctatus 386 443 0.770 1.5 0.4 ,)

Yellow petth Perca flavescens 309 342 1.159 52.2 15.5 299 292.5 -1.094 207 A9.0 1.003 202 82.0 0.994

. 202 82.0 0.994 198 80.5 1.037 198 80.5 1.037 205 90.5 1.050 212 92.5 0.965 200 82.0 1.025 198 88.5 1.140 000 81.5 1.019 207 87.5 0.996 194 71.0 0.972 212 91.5 0.960 210 93.0 1.004 210 97.0 t.047 206 81.0 0.927 114 78.0 1.068 208 88.5 0.963 207 89.5 1.009 216 111.0 1.101 203 87.5 1.046 295 96.0 1.138 212 104.5 1.097 180 67.0 1.149 217 111.0 1.086 212 91.5 0.960 193 72.0 1.001 208 86.5 0.961 192 74.5 1.052 118 12.5 1.186 206 89.0 1.018 111 99.5 0.913 190 73.0 1.064 7 8/19/78 Chinoon salmon Oncorhynchus ischawytscha s 734 4449 1.125 16.6 0.9 762 4994 1.044 Brown trout 5almo trutta 387 681 1.179 16.6 0.9 545 1816 1.007 2-58 soience servloes devieson

Table 2-16 (Contd)

Lennth Weight Condition P m enta9e G ten For Station Date Core n Name Scientific hame (m) gm Factor Station Month Gear 7 8/19/18 Lake trout Salveltaus ny ycush-~ 662 2497 0.861 66.7 3.5 Gill Net 618 18?f 0.667 730 3859 0.992 628 1816 0.733 568 1589 0.867 585 2270 1.134 657 2043 0.720 658 2724 0.956 44 0.4 0.469 100 3.5 Ceach Seine 23 8/18/78 Ratnoow smelt Osmerus mordan 42 0.4 0.540 37 0.3 0.592 35 0.3 0.700 36 0.2 0.42) 40 0.3 0.469 38 0.3 0.547

• 0.469 40 0.3 8/18/78 Yellow percn Perca 'favescens 57 1.9 1.026 2.1 0.4 24 Unid. minnows Notrop4s so. 57 f.3 0.701 97.9 20.4 72 3.2 0.857 66 2.3 0.a00 66 2.1 0.730 66 2.3 0.800 67 2.5 0.831 53 1.4 0.940 53 1.9 0.973 62 2.0 0.839 64 2.4 0.916 50 1.1 0.880 63 2.6  !.040 60 1.9 0.880 73 3.2 0.823 68 2.8 0.890 63 1.7 0.680 59 1.9 0.925 55 1.5 0.901 62 2.2 0.923 63 2.2 0.880 i

63 2.1 0.840 55 1.4 0.841 "l 58 1.6 0.820 64 2.1 0.801 60 1.8 0.833 69 2.6 0.791 64 2.3 0.877 57 1.7 0.914 56 1.6 0.911 62 2.1 0.881 59 1.9 0.925 64 2.3 0.877 52 1.4 0.996 75 3.5 0.830 57 1.8 0.972

$7 1.7 0.918 63 2.1 0.840 51 1.3 0.980 70 2.5 0.816 60 2,2 1.018 80 4.1 0.800

$3 1.5 1.007 53 1.4 0.940 66 2.4 0.835 58 1.6 0.820 48 1.2 1.085 25 8/18/18 Alewife Alosa pseudonare qus 46 1.1 1.130 82.0 32.3 48 0.9 0.813 46 0.8 0.822 45 0.6 0.658 58 1.4 0.71 7 51 0.8 0.603 60 1.9 0.879 44 0.4 0.470 51 0.9 0.678 47 0.4 0.335 45 0.4 0.439 2-59 solence servlees division

O Table 2-16 (Contd)

~ Percentage Catch For Length Weight Condition Station Date Caumen Name Scientiffc Name (nn) gm Factor $tation Month Gear 25 8/18/78 Alewife Alosa pseudohareasus 46 0.5 0.514 8each Seine 51 0.7 0.528 54 0.9 0.571 42 0.3 0.405 56 1.1 0.626 46 0.5 0.514 46 0.4 0.411 45 0.5 0.549 47 0.9 0.866 48 0.8 0.723 48 0.9 0.813 .

46 0.6 0.616 42 0.4 -0.540 46 0.7 0.719 50 0.8 0.640 54 0.9 0.508 45 0.5 0.549 44 0.3 0.352 45 0.4 0.439 42 *0.4 0.540 50 0.7 0.560 48 0.6 0.542 45 0.4 0.439 46 0.5 0.514 44 0.4 0.470 44 0.4 0.470 50 0.7 0.560 52 0.9 0.641 51 1.0 0.753 44 0.7 0.821 48 0.9 0.814 42 0.6 0.810 44 0.8 0.723 44 0.6 0.704 49 1.0 0.850 28 0.1 0.455 49 0.9 0.764 56 1.4 0.797 48 0.8 0.822 -

s}

45 0.7 0.768 43 0.6 0.754 47 0.9 0.867 42 0.5 0.674 46 0.8 0.822

. 42 0.6 0.809 27 0.1 0.508 51 1.2 0.905 47 0.7 0.674 44 0.7 0.822 42 0.6 0.810 l 46 0.8 0.822

! 52 1.1 0.782 47 0.8 0.771

! 43 0.6 0.755 44 0.6 0.704 49 1.0 0.850 47 0.9 0.867 42 0.6 0.810 35 0.3 0.700 27 0.1 0.508 25 8/18/78 Ye11ow Perch Perca fisvescens 73 3.8 3.977 10.1 4.0 83 5.6 0.979 48 1.0 0.904 76 3.4 0.550 47 1.0 0.963 80 5.0 0.977 50 1.2 0.960 53 1.4 0.940 48 0.9 0.814 Unid. minnows Notropis sp. 72 3.1 0.830 7.9 3.1 45 I.9 0.988 52 1.5 1.067 35 0.5 1.166 64 2.0 0.763 64 1.8 0.687 63 2.2 0.879  ;

17 8/20/78 Black bullhead !ctalurus meias 115 21.8 1.380 100 0.4 Electroshocker 125 24.8 1.270 18 8/20/78 Black bullhead !ctalurus me' n 114 20.5 1.384 100 0.9 2-60 solence servlees division

O Clip marks were noted for 19 of the salmonids. The details of the markings are shown in Table 2-17 Ichthyoplankton data for June, 1973 ;re shown in Table 2-18. The bulk of the catch was alewife, although several cyprinid (probably carp) and one prolarval percid (yellcw perch or johnny darter) were collected. No eggs or larvae were collected in the nearshore ponds. Densities ranged from 0.08 per cubic meter to 36.39 per cubic meter. The average egg density for alewives was 2.95 per cubic meter, while prolarval densities averaged 0.0457 per cubic meter. If the prolarvae move very little from where the eggs are deposited, this would yield a natural mortality rate from egg to prolarvae of 98.5 percent.

Incidental ichthyoplankton observations from Ponar dredge are shown in Table 2-19. Although all samples did not yield eggs, those which did yielded fr,m 19 to approximately 1.435 eggs per square meter. If it is assumed that the dredge collects eggs during the dredge's fall to the bottom as well as while on the bottom, an averago egg density of 6.3 eggs per cubic meter are present in the entire water column.

The vertically hauled cooplankton net, which yielded an average density of 2.95 i eggs per cubic meter, may underestimate egg density in the Bailly area of Lake Michigan.

2.7 WATER QUALITY 2.7.1 METHODOLOGY. In the Bailly Study Area, water quality parameters were measured in the lakt and in ponds both onsite and in the National Lakeshore area as designated by stations 1 through 22 on Figure 2-1. The water quality param-eters to be measured, the stations sampled, and the methods used and their ac-curacy appear in Table 2-20. Additionally, Table 2-21 lists each parameter and its Indiana water quality standard levels; where Indiana standards were not im-mediately available, U.S. Public Health Service or Federal Environmental Protec-tion Agency values were used.

All water quality samples for the spring sampling period were collected using either a Van Dorn whole-water sampler (or equiv ' nt) or a J-Z sterile sampler (for bacteria samples only).

2-61 solence servloes division

Table 2-17 Clip and Tagging Marks on Salmonids Collected in the Bailly Study Area, August 1978 ,

'l Length Weight species Station Date (nm) (gm)* Sex Sexual Condition Location of Clip Notes Lake troet 4 8/21/78 672 2724 F Mature Pelvic fin large eggs 774 4313 F Mature Pelvic fin Large eggs 650 2497 M Mature Adipose fin 672 2497 F Mature Adipose fin f,55 2270 M Mature Adipose fin Degenerate pectoral 682 2951 F Mature Adipose fin 687 2951 F Mature Adipose fin 737 3859 F Mature Adipose fin 695 2951 F Mature Adipose fin o Adipose fin 580 1589 M Ma ture-a Brown trout 4 8/21/78 473 1135 F Mature Pectoral Fin 344 545 M Innature Pectoral Fin Lake trout 7 8/19/78 662 2497 M Deflated Gonads Adipose and Pelvic fins {

618 1816 H Deflated Gonads Adipose and Pelvic fins 730 3859 M Deflated Gonads Adipose and Pelvic fins 628 1816 F Mature Pelvic fin 5 563 1589 M Matere Adipose fir.

! 657 2043 F Mature Adipose and Pelvic fin 8 653 2724 M Ma;ure Adipose fin e

• Weight measured in pounds and converted to grams k

0 U

U R

I e

0 3

y Q

@ Table 2-18 Ichthyoplankton Data, Bailly Study Area, June 1978 Station Species Life Stage Number T

Number /m lA Alewife Egg 1 0.28 IB Alewife Egg 25 6.94 1C Alewife Egg 131 36.39 ID Alewife Egg 19 5.28 Alewife Prolarvae 1 0.28 2A Alewife Prolarvae 2 0.28 2B Alewife Egg 4 0.56 Alewife Prolarvae 3 0.42 2C Alewife Egg 78 10.99 2D No catch 3A Alewife .3 20 1.68 Alewife Prolarvae 1 0.08 3B No catch 3C Alewife Egg 5 0.42 Alewife Prolarvae 1 0.08 3D Alewife Egg 1 0.08 4A Alewife Egg 1 0.28 4B Alewife Egg 1 0.28 4C Alewife Egg 1 0.28 40 No catch SA Alewife Egg (3 1.a3 Alewife Prolarvae 1 0.14 Percidae Prolarvae 1 0.14 .

(Yellow Perch or Johnny Darter)

SB Alewife Egg 15 SC Alewife Egg 21 Cyprinidae Egg 1 v... "

(aopears to be carp) 50 tm catch CA Ale.tife Egg 22 1.85 6 6B Alewife Egg 1 0.08 6C No catch 60 180 catch 7A Alewife 109 70 19.44 7B Alewife Egg 18 5.00 7C Alewife Egg 7 1.94 ',

Unidentifiable Egg 1 0.28 70 Alewife Egg 13 3.61 8A Alewife Egg 10 1.41 Alewife Prolarvae 1 0.14 8B Alewife Egg 10 1.41 8C No catch 80 Alewife Egg 6 0.85 9A Alewife Egg 11 0.92 9B Alewife Egg 28 2.35 Alewife Prolarvae 1 0.08 9C Alewife Egg 77 2.27 90 Alewife Egg 5 0.42 10A Alewife Egg 7 2.33 Cyprinidae Egg 2 0.67 (appears to be carp) 10B Alewife Egg 1 0.13 10C Alewife Egg 6 2.00 Prolarvae 1 0.33 100 Alewife Egg 4 1.33

• No catch in nearshore pond stations 17-21 samples.

No catch in pumped samples from stations 4 and 7.

2-63 soience services division i

Table 2-19 Incidental Ichthyoplankton Data, Bailly Study Area, June 1978 4

2 Station Species Life Stage Number Number /m 18 Alewife Eggs 6 115 2A Alewife Eggs 2 75 =1.435 25 Alewife- Eggs =75 =1.4 35 3A Alewife Eggs 8 153 3B Alewife Eggs = 50 =957 SA Alewife Eggs 2 40 =765 58 Alewife Eggs =25-30 =478-574 6B Alewife Eggs 1 19 M Alewife Eggs 2 38 76 Alewife Eggs 2 -38 BA Alewife Eggs 10 191 8B Alewife Eggs =25 =478 9A Alewife Eggs 1 19 98 Alewife Eggs 6 115 10A Alewife Eggs = 60 =1.148 Cyprinidae Eggs 1 19 10B Alewife Eggs =25 =478 Cyprinidae Eggs 9 172

)

i In situ parameters were measured using a YSI Model 59 temperature / dissolved-oxygen meter with remote stirrer, a Beckman solu-bridge conductivity meter, a standard four-quadrant Secchi disk and a Beckman field-model pH meter. Also-recorded at each sampling location were wind direction and speed, air tempera-ture, water color, percent cloud cover, and time of day. These ancillary meas-urements are useful in interpreting some of the biological data but are not con-sidered part of water quality data and are not presented in the tables; however, they are included in sections where they are used to help explain such factors as species composition.

[

Samples from the ash-settling basins (stations 13-16), the natural ponds (sta-i tions 17-21), and Cowles Bog (Station 21) were collected at mid-depth. Lake i Michigan samples from locations along the 15-foot contour (stations 1, 4, and 7) were collected from 1 meter below the surface: lake samples along the 30-foot contour (stations 2, 5, and 8) were collected from 1 meter above the bottom; and lake samples along the 50-foot contour (stations 3, 6, and 9) were collected 1 meter below the su ' ace, at mid-depth, and 1 meter above the bottom. All other lake samples (stations 10, 11, and 22) were collected 1 meter below the surface.

J 2-64 solence servlees division

O Table 2-20 Water Quality Parameters Measured in Vicinity of Bailly Study Area Passeeter Stattom %ethod Accuracy AQUAf1C Water Chemistry and sectoriology Ceneral Water Quality Alkalinity, tctal 1-21 T1tration II at 100 mg/t Calcium, soluble 1-21 eac 12 Atoetc absorption 10.05 mg/1 Chloride, total Auto analysis 2/33 at 5 mg/l Chlorire. total 1-21 enc 12 Titration Conductance, spec tite Conductivity bridge 51 at 50 smhoe Oxygen, dissolved 1-21 Winkler and polare- 20.1 es/t graphic Osygen, saturation 1-21 Calculation N/A Odor, threshold 1-21 esc 12 Threshold N/A Magnesium, soluble 1-21 ens 12 Atoste absorption to.00A mg/t Hardness 1-21 eac 12 Titration 2.91 at 232 mg/t pH 1-21 Electrode 20.1 pH Potassium, soluble 1-21 enc 12 Atomic absorption 20.005 mg/t Sodlum, soluble 1-21 enc 12 Atomic absorption 20.005 mg/t Dissolved solida, total 1-21 exc 12 Graviestric At at 100 mg/t suspended solids, tot al 1-21 eac 12 Gravimetric At at 100 mg/t Sulfate 1-21 enc 12 Colortsetrie 32 at 100 mg/t Temperature 1-21 The rmomet e r 20.1*C Turbidity 1-21 Nephelwnetric N/A Color. t rue 1-21 eat 12 St anda.d filters N/A Fluoride, soluble 1-21 ese 12 Distillation 81 at 800 g/t Aquatic Nutrients Ammonta, soluble 1-21 Auto analysis 0.31% at 8 agat/tN Nitrate, soluble 1-21 Auto analysis 0.591 at 2.5 maat/1N Nitrite, soluble 1+21 Auto analyste 0.591 at 2.5 vaat/1N organte nitrogen, total 1-21 Auto analysta 1.251 at 50 mg/tN Orthophosphate. soluble 1-21 Auto analysis 1.901 at 2 kgat/tP Phosphorus, total 1-21 Auto analysis 0.891 at 30 mg/IP

$lltes, soluble 1-21 Auto analysis 0.361 at 5 mg/45102 Trece Elements cadmium, total 13-21 Atomic absorption 20.005 mg/t Chroetua, soluble hezavalent 13-21 Auto analysts 20.143 at 0.10'es/1 Chromtus, total 13-21 Atomic absorption 20.002 as/t Copper, total 13-21 Atomic absorption 20.03 mg/4 iron, soluble 13-21 Atomic absorption 20.05 mg/t Manganese, total 13-21 Atomic absorption 20.01 mg/t Mercury, total 13-21 Atomic absorption 20.0002 mg/t Nicket, total 13-21 Atoute absorption 20.05 mg/t 2ine, total 13-21 Atomic absorption 20.01 as/t Lead 13-21 Atomic absorption t0.01 mg/l Indleators of industrial and Orlan te Cont entnation Bacteria, fecal coliform 13-21 Membrane filter N/A Bacteria, total coliform 13-21 Membrane filter N/A 31ocheatcal Orygen Demand 13-21 Wlahler and polaro= 20.1 mg/L graphie Hemane-soluble materials 13-21 Hexane estraction N/A organte Carbon, total 13-21 Combusttoe - IR N/A Phenols 13-21 Chlorof orm estraction 20.0001 mg/t Methy'ene '1ue-Active Substance 13*21 5pec t rop hot ome t rie 20.02 as/t

! Cyanide 13-21 Cyanide distillation 20.005 mg/L ,

Chestcal oxygen Demand 13-21 Titration 20.1 as/t

) Sediment J

cadetum, total 13-20 Atomic absorption 20.D05 og/t i Chromium, total 13-20 Atomic absorption 20.07 as/t i Copper, total 13-20 Atoets absorption 20.03 as/t iron, total 13-20 Atomic absorption 20.05 mg/t Lead, total 13-20 Atomic absorption 20.06 ag/t Manganese. total 13-20 Atomic absorption 20.01 mg/t Mercury. total 13-20 Atoste absorption 20.0002 mg/t (flameless) l Nicket, total 13-20 Atomic absorption 20.05 mg/4 1elenium, total 13-20 Atomic absorption 20.0003 as/1 vanadium, total 13-20 Atomic atsorption 10.002 as/t Zinc. total 13-20 Atoste absorption 20.01 og/t Phosphorus, total 13-20 Auto analysis ti.9%I at 2 kget/t i l

l 1

, 1 2-65 science services division

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l 4

Table 2-21 Water Quality Values Defined by Indiana Stream Pollution Board, S U.S. Public Health Service, or Federal EPA and Applicable /

to 1.ake Michigan in Bailly Study Area m - al hr mality t'n i t s tndiana. USPMS or EFA levels Alkalinity as/t 30-500 range, whatever la of natural origin III Calciue ag/1 No limits defined Chlorides ag/l 15 single values. 10 monthly average II)

Chlorine og/t .003 mg/t (1,2)

Conductivity wahos 4800-1200 micronhos/cm (at 25*C)III AFHA units 5 monthly average (I)

Color 15 single vag assimum.

01ssolved Ozygen ag/ t Not 1 5 mg/t Dissolved Osygen og/t 80% sinimum(I)

Fluorides ag/t Not to exceed 1.0 at any time (I)

Mardness og/t 0-500 range, natural origin (2)

Magnesium aq/f No limits defined (2)

Odor oh r units pos-neg Single value 8 - daily ave 4II) pH r1 units 7.5-8.5(I)

Fotasslum ag/L No limits defined (2)

Sodium ag/ t No limits defined (2)

Total Dissolved Solida eg/t 172 (Lake Michigan monthly avs) 750 elsewhere(I) 200 (1.ake Michigan single value) 1000 elsewhere Total Suspended Solids ag/t 80(2)

Sulfate og/t 50-single valuel 26-monthly average Water Temperature *C 3*F above existing 1000 ft from discharge or II) 45'(Jan-Mar) 55'(Apr) 60*(May) 70*(Jun) 80*(Jul-Sep) 65*(Oct) 60*(Nov) 50*(Dec) whichever is lower FTU None other than natural originIII Turbidity Aquatic Nutrient III ag/4 0.05 sin e value. 0.02 monthly everage Ammonta N1trates as/1 10 mg/t( - Freshwater (Public Supply)

Nitrites ag/l 1 as/g(2) - Fresh er (Public Supply)

Organic Nitrogen ag/t No limits defined

• Orthophosphate 8 ag/t No limits defined - presumably less than total F. .

Total Phosphorus eg/t 0.04 single value. 0.03 monthly average )

Silicates eg/t No limits defined Trace Elements st/t Not to exceed 0.05 at any time (I)

Arsenic, total as/t Not to exceed 0.01 at any time (I)

Cadatum, total Chrostum, hezavaient og/t Not to exceed 0.05 at any ties (I)

Chroutum, tatal og/1 <0.03(2)

Copper, total as/t 1.0(2) og/l .30 in .15 monthly average (I) tron. soluble 0.3g)alevalue.

I Iron, total og/t og/t 3ot exceed 0.05 at any time (I) 1.ead, total O.05g)

Manganese, total ag/ t- '

Mercury, total as/4 Not u exceed 0.0005 at any time (2) 1/50 96 hr. TL * .5-2 mg/ t(3) sickel, total as/t Selenium, total og/l Not toexceed30*.00. at.n, ti.e(1)

Vanadium, total as/t No limits defined (2)

Zinc. total as/1 5(2)

Indicators of Industrial and Organk C ntamination 20/100 (t.ake Michigan open water) 200/100 mi at beachen IU Secterla, fecal califors f/100 et (ased on geometric mean of 5 samples f/100 si <100/100 et desirable at allowed (2) tacteria, total co11 tors Noprescribed11 mite (2g0.000/100 Biocheetcal Oxygen Demand as/t No prescribed limite III Cheetcal Oxygen Demand ad/t any time (I)

Cyanide og/ L Not to exceed .0 at as/t Virtuallyabsent)2I Nemane, soluble esterial .003 single value. .001 monthly average (1)

Phenote es/t Methylene Blue Active Sub-stances as/t 0.5(2)

No prescribed 11mits III Total organic carbon og/4 (1) Indiana Regulation SPC 4R or SFC 1R-3 (2) USFMS Drinking Water Standards (1961)

(3) EPA Water Quality Criteria Data Sook - Vo!, 3 (1971) /

2-66 science servlees division

O 2.7.2 RESULTS AND DISCUSSION - JUNE 2.7.2.1 General Water Quality Parameters, Lake Michigan. Twenty general water quality parameters were measured at 22 sampling locations in Lake Michigan and at 9 pond stations during June 1978.

Data from sampling stations in Lake Michigan (stations 1 through 12 and 22, Table 2-22) showed that all parameters except plume t'emperature (Station 22),

dissolved oxygen, chloride levels, pH, and total dissolved solids (TDS) were within the guidelines established by the Indiana Stream Pollution Control Board (Table 2-21). Plume temperatures 1,000 feet from.the point of discharge ex-cceded ambient temperature (calculated from stations 3S, 6S, and 9S as 15.8'C) by 8.2*C, Dissolved oxygen levels at Station 9B (at 50-foot depth) were 7.0 milligrams / liter and 65 percent saturation. Station 9B was the only location where this value occurred, and concentrations were sufficient to maintain a fisheries population. Chloride levels, which have been rising throughout Lake Michigan, averaged 10.5 milligrams / liter in June 1978, 0.05 milligram / liter in excess of standards. As the valuer are ubiquitously high, the effect of the power plant is negligible. The pH levels exceeded or were below the 7.5 to 8.5 allowable range at several stations. The stations with aberrant values are at least 2,500 feet from the Bailly Station discharge and thus no effects on this parameter could be attributable to plant operation. Total dissolved solids levels exceeded the 172 milligrams / liter recommended value by approximately 17 milligrams / liter. Values are extremely variable, although nearfield values are somewhat higher than those at farfield stations. Values at the discharge (Sta-tion 10) were higher than standards (recorded value of 181 milligrams / liter), but values at Station 22 (1,000 feet from the discharge in the plume centerline) were well within standards (recorded values of 145 and 146 milligrams / liter). The ef-feet of Bailly Station operation on this parameter may therefore be minimal.

Other general water quality parameters, as noted previously, were well within standards. Values were similar to those recorded during previous sampling pe-riods.

2.7.2.2 General Water Quality Parameters, Ponds. All general water quality parameters were monitored in both the natural (stations 17-21) and ash settling (stations 13-16) ponds. Numerous differences existed netween the ash ponds and the natural (interdunal) ponds. These are discussed next.

2-67/68 soience services division

@2.7.2.3 Nutrients, Lake Michigan. As shown in Table 2-23, ammonia levels

)

in June 1.978 exceeded the monthly average ISPCB specifications, although the '

single value maximum of 0.05 milligram / liter was exceeded only in replicates 12a and 22b. The values appear to be uniformly above standards, with no ob-vious tie to plant operation. Nitrate and nitrite levels were equal to or less than those recorded in June 1977, while organic nitrogen levels were somewhat higher. Orthophosphate levels, while fractionally higher, remained at levels just above limits of detectability, while total phosphorus levels were slightly higher than in June 1977. Silica levels were 1/4 to 1/3 those of June 1977 and indicate probable silicate utilization in planktonic organisms (i.e., diatom frustules).

2.7.2.4 Nutrients, Ponds. Except for organic nitrogen, nitrogen levels in the settling ponds were lower in June 1978 than in June 1977. This is the same situation that existed in June 1976 (i.e., all June 1976 nitrogen levels except organic nitrogen were lower than in June 1977). Orthophosphate levels (Table 2-23) were higher in the interdunal ponds in June 1978 than in June 1977, while total phosphorus levels were similar. Silica levels, which averaged 6.30 milli-i grams / liter in June 1977, averaged 2.25 milligrams / liter in June 1978. Levels J in the ash-settling ponds (stations 13-16) were approximately equal to each other (0.91 to 1.56 milligrams / liter range, 1.28 milligrams / liter mean). Mean levels in Pond B were slightly higher (1.40 milligrams / liter), Pond C slightly lower (0.57 milligram / liter). Cowles Bog values, historically higher, averaged 11.23 milligrams / liter.

Although certain nutrient levels within the natural ponds (notably ammonia) ex-ceeded ISPCB specifications for effluents to Lake Michigan, no external pollu-tion sources were noted, and observed levels were not obviously detrimental to the indigenous flora and fauna.

1 2.7.2.5 Indicater of Industrial and Organic Contamination, Ponds. As 'hown s I in Table 2-24, fecal coliform levels in the ash-settling and natural ponds were relatively low, indicating an absence (or extremely low levels) of human con-tamination although the total coliform levels, particularly in Cowles Bog, in-dicate significant coliform bacterial inputs at some locations. Because of the locations of the high levels, the Bailly Station does not appear to be impli- ,)

cated.

2-72 soience servloes division I

2.7.2.3 Nutrients, Lake Michigan. As shown in Table 2-23, ammonia levels in June 1978 exceeded the monthly average ISPCB specifications, although the single value maximum of 0.05 milligram / liter was exceeded only in rr.plicates 12a and 22b. The values appear to be uniformly above standards, with no ob-vious tie to plant operation. Nitrate and nitrite levels were equal to or less than those recorded in June 1977, while organic nitrogen levels were somewhat higher. Orthophosphate levels, while fractionally higher, remained at levels just above limits of detectability, while total phosphorus levels were slightly higher than in June 1977. Silica levels were 1/4 to 1/3 those of June 1977 and indicate probable silicate utilization in planktonic organisms (i.e., diatom frustules).

2.7.2.4 Nutrients, Ponds. Except for organic nitrogen, nitrogen levels in the settling ponds were lower in June 1978 than in June 1977. This is the same situation that existed in June 1976 (i.e., all June 1976 nitrogen levels except organic nitrogen were lowe.t than in June 1977). Orthophosphate levels (Table 2-23) were higher in the interdunal ponds in June 1978 than in June 1977, while total phosphorus levels were similar. Silica levels, which averaged 6.30 milli- 1 grams / liter in June 1977, averaged 2.25 milligrams / liter in June 1978. Levels in the ash-settling ponds (stations 13-16) were approximately equal to each other (0.91 to 1.56 milligrams / liter range, 1.28 milligrams / liter mean). Mean levels in Pond B were slightly higher (1.40 milligrams / liter), Pond C slightly lower (0.57 milligram / liter). Cowles Bog values, historically higher, averaged 11.23 milligrams / liter.

Although certain nutrient levels within the natural ponds (notably ammonia) ex-ceeded ISPCB specifications for effluents to Lake Michigan, no external pollu-tion sources were noted, and observed levels were nat obviously detrimental to the indigenous flora and fauna.

2.7.2.5 Indicators of Industrial and Organic Contamination, Ponds. As 'hown s

in Table 2-24, fecal coliform levels in the ash-settling and natural ponds were relatively low, indicating an absence (or extrem 'y low levels) of human con-tamination although the total coliform levels, particularly in Cowles Bog, in-dicate significant coliform bacterial inputs at some locations. Because of the locations of the high levels, the Bailly Station does not appear to be impli- j cated.

2-72 science servlees division

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levels remained at levels comparable to June 1977. Because of the greater or- )

ganic loadings in Cowles Bog, these samples have traditionally yielded higher values for these three measurements; this was also the case in June 1978.

l Levels for hexane-soluble materials, phenols and methylene-blue-active-sub-stances were below the limits of detectability in June 1978. Cyanides, al-though not a required parameter, were measured and found to be present at or just slightly above detectability limits but well below the 0.01-m1111 gram /

liter allowable standards.

2.7.2.6 Trace Elements, Ponds. A total of 10 trace elements were measured in the nearshore ponds in June 1978 (Table 2-25). Of these, total chromium, lead, and mercury levels were below limits of detectability. Soluble (hexa-valent) chromium levels were at or just slightly above detection limits.

Cadmium, which was found in the natural ponds only at Station 17 during the l June 1977 sampling, was found at all five stations in June 1978. Levels, how-i ever, remained low. Copper, which was found in significant quantities at ash- )

settling pond stations 14 and 15 in June 1977, was uniformly low (less than O.004 milligram / liter) in June 1978. Iron, a major component in June 1977 (0.037 to 0.600 milligram / liter), never exceeded 0.015 milligram / liter in June 1973. Manganese concentrations were equally low, not exceeding 0.006 milligram / liter in June 1978, although levels up to 0.691 milligram / liter had been recorded in June 1977. Zinc, another element recorded in significant quantities (up to 2.970 milligrams / liter) in June 1977, reached maximum levels of 0.103 milligram / liter in June 1978. A combination of changes in the chemi-cal composition of the coal used as well as decreases in airborne particulate fallout from nearby smelting facilities is suspected as reasons for the de-cline.

2.7.3 RESULTS AND DISCUSSION - AUCUST 2.7.3.1 General Water Quality Parameters, Lake Michigan. General water quality levels for August (Table 2-26) were compared to relevant standards (Table 2-21). All but one of the parameters measured were within standards.

Chlorides, which had exceeded the 10-milligrams / liter standard in June, J 2-76 science servlees division

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decreased to approximately 8.7 milligrams / liter. Only 1 station (9S, repli-cate a) exceeded the 10-milligrams / liter standard (value of 12.8 milligrams /

liter). The other replicate of station 9S yielded a value of 8.5 milligrams /-

liter.

3 Chlorine levels were below the limits of detectability, while the metals (cal-i cium, magnesiua, potassium, and sodium) also decreased from June 1978 to August i

1978 although levels of these metals were higher (greater than 20 percent) than in August 1977. The differential is thought to be a normal fluctuation.

Oxygen concentrations ranged from 9.2 to 9.6 milligrams / liter (percentage sat-uration 91 to 120 percent), while temperatures were from 11.5'c on the bottom in 50 feet of water to 27'C at the discharge. A distinct thermocline was present, with a top-to-bottom temperature differential of 6.5 to 10.5'C. Lake samples were without odor and basically colorless, although dissolved solids were in excess of 1000 milligrams / liter in some samples, with higher readings at the nearfield stations.

Turbidity values were low, as were suspended solids levels. F1.uoride levels were also low, averaging 0.12 milligram / liter.

2.7.3.2 Ceneral Water Quality Parameters, Ponds. Alkalinity levels in the ash-settling ponds were lower than in June 1978 but higher than in August 1977.

Levels in the natural ponds, while higher than in June 1977, were comparable to those of August 1977. Chloride levels decreased from the June 1977 sampling period, and only one station (18) yielded values (10.4 milligrams / liter) in ex-cess of the standards (10.0 milligrams / liter). Chlorine levels remained below the limits of detectability. Conductivity levels in the ash ponds averaged 585 micrombos, while natural pond levels averaged 440 micromhos. These levels are comparable with those of August 1977.

Dissolved oxygen levels in the ponds ranged from 5.3 milligrams / liter to mor than 15.0 milligrams / liter (the limits of the dissolved oxygen instrumentation),

and saturation levels ranged from 65 to over 100 percent. The low value was re-corded at Station 19; 5 milligrams / liter is the minimal level to support a warm-water fishery. A high value in Cowles Bog (Station 21) was also recorded in June 1978 and would appear to indicate strong plankton growth in that location.

2-81 solence servlees division

The pa. ievels in the ponds ranged from 6.7 at Station 17 to 8.0 at Station 20. -

This range is similar to that recorded in August 1978. Temperatures in the ponds ranged from 26.0 to 29.0*C, 4 to 9'C higher than the values in Lake Michigan.

Color levels in the ash-settling ponds were virtually negligible (1 Pt-Co unit),

while those in Coeles Bog averaged 300 Pt-Co units. Values in Ponds B and C (stations 17-18 and 19-20) averaged 12.5 and 42.5 Pt-Co units, respectively.

These values compare favorably with values recorded during the previous June.

Turbidity and suspended solids levels were higher in the ponds than in Lake Michigan, as in previous sampling periods. No values considered abnormally high were recorded.

Fluoride levels were comparable with data from previous years.

2.7.3.3 Aquatic Nutrients, Lake Michigan. As shown in Table 2-27, nitrite and orthophosphate levels were generally at or below limits of detectability, although some stations (e.g., SS, rep a; SB, rep a for orthophosphate) regis-tered measurable values. A comparison of the August 1978 data with August 1977 data (TI, Summer 1977 Quarterly Report) indicates that low summer nitrite and orthophosphate concentrations are common phenomena.

Ammonia concentrations in August 1978 ranged from 0.002 to 0.050 milligram /

liter, while the range in August 1977 was 0.004 to 0.037 milligram / liter. Aver-age values for August 1977 and August 1978 were 0.019 and 0.015 milligram / liter, respectively. The two years compare quite favorably.

Nitrate concentrations in August 1977 ranged from 0.11 to 0.20 milligram / liter (mean = 0.14 milligram / liter) while August 1978 concentrations ranged from 0.14 to 0.19 milligram / liter (mean = 0.16 milligram / liter). As with the nitrate data, the two years compare favorably.

Organic nitrogen levels varied more widely than nitrate levels. August 1977 values ranged from 0.04 to 0.24 milligram / liter (mean = 0.14 milligram / liter),

while August 1978 values had a range of 0.09 to 0.45 milligram / liter (mean =

0.24 milligram / liter). Although the 1978 mean value is 71 percent higher than )

that of 1977, concentrations are still low but do indicate a difference in 2-82 science servlees divieson

l biological compound levels (amino acids, polypeptides, proteins, and their degra-dation products - a possible indication of sewage or industrial effluent) between the two years. Because of the ubiquitous nature of the values (i.e., widespread throughout the Bailly Study Area) and the very low values in the ash-settling ponds (stations 13-16, which receive some of the Bailly Station sewage plant treated ef-fluents), Bailly Station operation is not thought to be related to the 1977 to 1978 increase in organic nitrogen, j

As mentioned previously, orthophosphate levels remained at very low concentra-tions in 1978. Total phosphorus levels were somewhat greater, ranging from 0.005 to 0.020 milligram / liter (mean = 0.013 milligram / liter). Values in 1977 ranged from less than 0.002 to 0.245 milligram / liter. Discounting the two ex-tremely high values (0.240 and 0.245 milligram / liter) as an anomaly, the mean concentration in August 1977 was 0.007 milligram / liter, slightly below the 1978 level. The difference should not markedly affect plankton growth, as the ac-tive orthophosphate component levels remain low.

Silica levels, which have been decreasing in Lake Michigan since the 1960's (Verduin 1977), remained at relatively low levels in 1978. Verduin noted a concentration of approximately 2.0 milligrams / liter in Lake Michigan sam-ples in 1962. In August 1977 the average concentration measured at the NIPSCo Bailly site was 0.45 milligram / liter, while August 1978 concentrations averaged 0.23 c.illigram/ liter, continuing the trend of decreasing silicate levels.

2.7.3.4 Aquatic Nutrients, Ponds. Ammonia nitrogen levels were higher in the ash-settling ponds (stations 13-16) than in either the natural ponds (sta-tions 17-21) or in Lake Michigan (Table 2-27). This was also the case in August 1977. Ash-settling pond concentrations averaged 0.241 milligram / liter; Pond B samples, 0.016 milligram / liter; Pond C, 0.011 milligram / liter; and Cowles Bog, 0.014 milligram / liter. As compared to August 1977, 1978 ash-settling pond val-ues were higher, Pond B values higher, Pond C values lower, and Cowles Bog val-ues lower.

Nitrate 1centrations, as in Augt.st 1977, were found in measurable concentra-tion only in the ash-settling por.ds. Nitrite concentrations followed a similar trend, except that this compound was also found in low concentrations in the ash-settling ponds. Significant concentrations (mean = 0.018 milligram / liter) 2-83/84 science servloes division

O were measured only in Cowles Bog samples. The levels measured in Cowles Bog are not considered harmful and do not approach ISPCB standards (see Table 2-21).

Organic nitrogen levels were low, as expected, in the ash-settling ponds, higher in Ponds B and C, highest in Cowles Bog. The Bog area has relatively large amounts of decomposable organic matter, and the higher values are common in this location.

Ortho- and total phosphate levels were similar to organic nitrogen. Levels of both phosphate forms were low in the ash-settling ponds. Significant levels of orthophosphate (mean = 0.018 milligram / liter) were found only in Cowles Bog.

Total phosphorus levels were also highest in Cowles Bog, although Ponds B and C yielded 0.013 and 0.018 milligram / liter of this compound respectively. Levels recorded in August 1978 were similar to those recorded in August 1977.

Silica concentrations, as in August 1977 and other previous sampling periods, were higher in the ash-settling and natural ponds than in Lake Michigan. Ash-settling pond values in August 1978 averaged 1.97 milligram / liter as compared to 0.23 milligram / liter in August 1977. Pond B values averaged 2.44 milligrams /

liter, Pond C, 2.04 milligrams / liter, and Cowles Bog, 22.1 milligrams / liter.

Although both 1977 and 1978 values had wide station-to-station variability, val-ues were similar between the two years.

2.7.3.5 Indicators of Industrial and Organic Contamination, Ponds. e in June 1978, fecal coliform levels in the ash-settling ponds were relats ' low (Table 2-28). Stations 14 and 15 yielded no fecal coliforms, while ste..ons 13 and 16 yielded 500/100 milliliters and 87.5/100 milliliters, respectively. Val-ues in the natural ponds (stations 17-21) were notably higher. Numbers in Pond B (stations 17-18) averaged 15,263/100 milliliters, approximately 54 percent of the 28,375/100 milliliters recorded for total coliform bacteria in this pond. Val-ues in Pond C averaged 2,800 fecal coliform and 26,625 total coliform /100 milli-liters, while Cowles Bog values averaged 22,250 fecal coliform and 46,500 total co11 form per 100 milliliters. The fecal-to-total-coliform ratio on Pond B and Cowles Bog (Cowles Bog coliforms were 48 percent fecal in nature) indicate sig-nificant inputs of animal (warm-blooded) origin. This has occurred in past sam-pling periods and appears to be related to migratory bird passage through the

-87 science servloes division l

4 i

Table 2-28 o -

t Indicators of Industrial and Organic Contamination, Nearshore Ponds, Bailly Study Area, August 1978  ;

4 Station .t.

i Parameter Units Rep 135 145 155 165 175 185 195 205' 215 i Bacteria, fecal coliform Es./100 at a 175 0 0 50 30.750 10.350 975 850 21.250 825 0 0 125 9.050 10.900 725 8.E50 23.250.

I Bacteria, total coltform E0./100 at a 2,000 50 150- 2.950 36.750 30./50 25.750 33,750 58.000 i 4

b 2.275 100 225 3.50* ' 13.750 32.250 22.750 24.250 35.000 i

Blochemical oxygen og/t a .I 1 1 1 1 1 1 1 9 demand b -1 1 1 1 1 1 1 1 8 -

t Chealcal oxy 9en demand ag/ r. a <2.0 <2.0 <2.0 3.5 4.5 4.0 10.3 1?.2 33.8 ^[

b 2.0 <2.0 <2.0 3.0 5.0 4.0 11.7 12.2 34.0 Hexane soluble materials ag/t a <C.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 3.6 b <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 <0.1 0.1 Total organic carton og/t a 3.2 3.3 4.5 7.2 6.3 7.0 16.0 14.4 37.8 b 3.6 3.7 3.9 3.7 6.6 6.6 16.4 18.0 48.2 N

8 rhenols mg/t a <0.005 <0.005 <0.005 40.005 <0.005 <0.005 <0.005 <0.005 <0.005 l

$ b <0.005 <0.005 <0.005 <0.005 <0.005 <0.005 <0.005 <0.005 <0.005 i

Nthylene - blue og/t a <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 active substances b <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 i

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area. It is not known if a migration was occurring during the August sampling.

November data will be closely examined to see if the high fecal coliform trend continues.

Biochemical oxygen demand (BOD) values for the ash-settling ponds, Pond B and Pond C, were all extremely low (1 milligram / liter). Values for Cowles Bog were somewhat higher (mean = 8.5 milligrams / liter) . Similar trends were observed for chemical oxygen demand (COD) and total organic carbon (TOC).

Hexane-soluble materials were below limits of detectability in all but Cowles Bog samples, which averaged 1.8 milligrams / liter. The source of the hexane-soluble materials (oils and greases) could be such things as natural ple.nt or animal compounds or artificial sources such as road or automobile oils or greases.

As in many of the previous sampling periods, concentrations of phenols and methy-lene-blue-active substances (surfactants such as detergents) were below the limits of detectability.

2.7.3.6 Trace Elements, Ponds. Concentratione of 10 trace elements were budSuted at nine pond stations in August 1978 (Table 2-29). Chromium levels were below (with two exceptions) detection limits, the two exceptions being replicates c and b of Station 18 which yielded 2 parts per billion of total chromium. Mercury levels were equally low, with only one value above detec-tion limits. The one value above detection limits was only 0.1 parts per bil-lion above those limits.

Cadmium levels were notable only in the ash-settling ponds and Station 17 of Pond B. Concentration in the remaining samples was below detection limits.

Copper levels were low in all samples (less than or equal to 0.007 milligram /

liter). Lead and manganese levels were similarly low (less than or equal to 0.010 and 0.050 milligram / liter, respectively). The high ranganese values were detected at only one ash-settling pond (No. 15).

Iron concentrations were low in the settling ponds, but higher in the natural ponds. Natural pond iron values were comparable with those of previous years.

Nickel and zine concentrations were highest in the settling ponds, particularly stations 15 and 16. Values in Pond C and Cowles Bog (less than or equal to 3 parts per billion) are thought to reflect background levels of these two elements.

2-89 solence services division

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2-90 solence servlees division

O 2.7.3.7 Trace Elements in Sediments, Ponds. As indicated in Table 2-30, total phosphorus and 11 trace elements in August samples collected from the bottom of the natural and settling ponds were analyzed. Phosphorus concentra-trations in Ponds B and C averaged 0.085 milligram / liter. The differential be-tween the two pond types is consistent with, although lower than, the differen-tial recorded in the summer of 1977.

Cadmium levels were present in settling ponds 2 and 3 (stations 15 and 16) and at natural pond station 17. Levels were not present elsewhere in measurable quantities. Chromium, which was below detection limita in the water samples, was also below detection limits in the sediment samples.

Copper, lead, mercury, nickel, and selenium concentrations were approximately equal in both the ash-settling and natural ponds. Iron levels were low in the first two ash-settling ponds, were present at levels of 1 to 2 milligrams / kilo-gram at ash-settling pond stations 15 and 16 as well as in the natural ponds.

The observed levels in the natural pond sediment samples appear to be the nor-mal (" background") concentration of this element. The same may be said of the copper, lead, mercury, nickel, and selenium levels.

Vanadium was present in measurable quantities only in Pond B sediment samples.

As none was present in Summer 1977 samples, this element will be closely moni-tored in future samples to determine if it persists.

Zinc concentrations were present primarily in the ash-settling ponds and at Station 17 in Pond B. This concurs with water sample data and appears to in-dicate concentrations in the coal-ash (with a potential for leaching of this material into Pond B).

Percent solids ranged from 49 percent in Pond C samples (high organic and water content) to 90 to 93 percent in the ash-settling ponds (low organics and water).

l l

l l

l l

2-91 solence servlees division l

Table 2-30 Trace Elemants in Sediment Samples from Nearshore Ponds, Bailly Study Area, August 1978 Station 16 17 18 19 20 Paraneter Unit Rep 13 14 15 0.016 0.027 0.074 0.071 0.079 0.067 Phosphorus mg/kg a 0.005 0.015 0.094 0.008 0.013 0.010 0.031 0.070 0. l ti7 0.058 b

0.045 <0.003 0.013 <0.003 <0.003 <0.003 Cadmium mg/kg a <0.003 0.028 0.008 <0.003 0.003 <0.003 <0.004 <0.003 b <0.003 0.031

<0.003 <0.003 <0.003 <0.003 <0.003 <0.003 Chromium mg/kg a <0.003 <0.003

<0.003 <0.003 <0.003 <0.003 <0.003 <0.003 b <0.003 <0.003 0.034 0.029 j mg/kg a 0.017 0.015 0.016 0.021 0.030 0.019 Copper 0.021 0.027 0.029 0.031 i b 0.015 0.01 6 0.021 0.018

<0.003 0.228 1.352 1.001 0.564 1.150 1.245 N Iron mg/kg a <0.003

<0.003 1.578 1.322 1.728 3.740 3.119 1.660 E b 0.013

~ <0.016

<0.014 <0.013 <0.013 <0.013 <0.017 <0.014 <0.019 Lead mg/kg a

<0.016

<0.013 <0.013 <0.013 <0.313 <0.015 <0.017 <0.016 b

0.100 0.228 0.011 0.019 0.037 0.214 0.140 0.079 Manganese og/kg 4 0.178 0.041 b 0.118 0.233 0.026 0.008 0.027 0.264

<0.0005 <0.0005 <0.0005 <0.0005 <0.0005 <0.0005 <0.0005 Mercury mg/kg a <0.0005

<0.0005 <0.0005 <0.0005 <0.0005 <0.0005 <0.0005 <0.0005 <0.0005 b

0.047 0.027 0.015 0.035 j Nickel mg/kg a 0.022 0.048 0.053 0.027 '

0.015 0.052 0.058 0.013 0.048 0.033 0.019 0.006

1. b

9. <0.019 <0.016

<0.017 <0.014 l0 Selen,um mg/kg a b

<0.014

<0.013

<0.013

<0.013

<0.013

<0.013

<0.013

<0.013 <0.015 <0.017 <0.016 <0.016 e 0.025 <0.008 <0.006 mg/kg a <0.005 < 0'. 005 <0.005 <0.005 0.060 e Vanadium

<0.005 0.033 0.030 <0.006 <0.006 8 b <0.005 <0.005 <0.005 3 0.011 0.004 0.029 mg/kg a 0.030 0.235 0.332 0.122 0.295 0 Zinc 0.185 0.017 0.010 0.022 8 b 0.031 0.297 0.359 0.094 90.7 92.3 89.7 90.3 65.8 59.6 49.1 63.9

&  % Solids  % a 92.6 70.2 64.7 52.2 71.5.

i b 90.9 90.1 90.7 e

0

• 2 Results expressed as mg/kg dry weight to lowest level of sensitivity available from instrumentation on day of analysis.

(. g y

2.8 LITERATURE CITED American Public Health Association. 1971. Standard Methods for the Examina-tion of Water and Wastewater. 13tb edition. APHA, Washington, D.C.

xxxv + 874 p.

Cassie, R.M. 1963. Microdistribution of plankton. Oceanography and Marine Biology: An Annual Review, 1:223-252.

Environmental Protection Agency. 1971. Water quality criteria data book, Vol. 3. Effects of chemicals on aquatic life. 526 p.

. 1973. Biological field and laboratory methods for measuring the quality of surface waters and effluents.

Edited by C.E. Weber. Nat. Env. Res. Center, Cincinnati, Ohio, 45258.

Indiana Stream Pollution Control Board. 1972. Regulation SPC 4R, Lake Michigan and contiguous Harbors.

. 1973. Regulation SPC IR3, Water quality standards for waters of Indiana.

Nauwerck, A. 1963. Die bezeihungen swischen zooplankton und phytop'iankton in: See Erken. Symb. Bot. Upsal. 17(5):1-163.

Patrick, R., M.H. John and J.H. Wallace. 1954. A new method for determining the pattern of the diatom flora. Bull. Philadelphia. Acad. Nat. Sci.

259:1.

Rohde, W., R.A. Vollenweider and A. Nauwerck. 1958. The primary production and standing crop of zooplankton, in: Perspectives in Marine Biology (edited by A. A. Buzzati-Traverso), Univ. of California. 299-322 p.

Strickland, J.D.H. 1960. Measuring the production of marine phytoplankton.

Fish. Res. Bd. Canada Bull. 122:1-172.

Talling, V.F. and D. Driver. 1963. Some problems in the estimation of chloro-phyll a,in phytoplankton. Proc. Conf. of Primary Productivity Measure-ment, Marine and Freshwater, Hawaii 1961. U.S. Atomic Energy Comm.

TID-7633:142-146.

2-93 solence servlees division I

-m Texas Instruments Incorporated. 1975. Summer 1975 quarterly report, Bailly .

Nuclear-1 site, encompassing June-August 1975. Prepared for Northern ]l!

Indiana Public Service Company.

l 1

. 1976. Summer 1976 quarterly report, Bailly Nuclear-1 site, encompassing June-August 1976. Prepared for Northern Indiana Public Service Company.

. 1977. Summer 1977 quarterly report, Bailly Nuclear-1 site, encomptssing July-September 1977. Prepared for Northern Indiana Public Service Company.

. 1978. 1977-1978 Annual report. Bailly Nuclear-1 site, encompassing April 1977-March 1978. Prepared for Northern Indiana Public Service Company.

United States Public Health Service. 1962. Drinking Water Standard for United States Waters.

Verduin, J. 1977. Testimony for Commonwealth Edison Company Zion Generating Station, by Dr. Jacob Verduin, Southern Illinois University.

Vollenweider, R.A. 1974. A manual on methods for measuring primary production in aquatic environments. Published for the International Biological Pro-gramme as IBP Handbook No. 12. Blackwell Scientific Publications. Oxford, 2nd Edition. xvii + 225 p.

1 2-94 science servloes division

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APPENDIX A PlWTOPLANKTON DENSITY REPLICATE REPORTS JUNE 1978 e

0 .

1 0

V I

a ir e

U i

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3

SREPORT o

IUNIT 8.

LASEL1x*f40RTHERN INDIAHA PUBLIC SERVICE COMPANY (497203*,

LAEEL2:'BAILLY GENERATIt4G PLAl(T*.

LASEL3 'PHYTOPLAt*Tott DEt4SITY'.

LABEL 4:'HUMBER CF CELLS PER LITER *.

RLAB1s' REPLICATE REPORT *.

RLAB x'STATI0tt REPORT * .

RLAB3:' STATION REPORT'.

RLAS4:' SITE

SUMMARY

SPEC 1:4,999.5 ' RAH'.'YES'.3.4.

SPEC 2=8.999.4.* RAH','YES'.3.7.

SPEC 3:7.999.4.' rat 4*.'YES'.3.7.

g SPE Ct.:6.9 9 9.6. ' R Af 4 ' , ' Y E S ' . 3. 3.

REPTOT:60.

LSTAT=1.

nAxTxA=500.

SID = STATION NUMEER GEARTB:56.1.0. 11 = STATION 1 #FPLICATE 1 ISIzt=2:a0 12 = STATION 1 REPLICATE 2 LISTz5.

0 0 SUMLEv=1.-l.-l.0.0.

JCT =30.

O STUNITx20.

HLIST:0.

I STFILE 1.

o O

N00ULE='G5656060'.

{ 10I0:0.

$ JCT 2:31, 3

LEtD L. \_) . _ - _ _ _ _ _ _

HORTHERti IPOI AttA PUBLIC SERVICE CottPAtif 149720)

DAILLY GENERATIt:G PLAHT FHrTOPLANKTCt1 DEtiSITY t:UtOER OF CELLS PER LITER REPLICATE FEPORT PC TC GC LOC 5 56 56 0 0 00 DURATICt1 TOW sat 1P VOL Wit;D CUREllT TEttP SID DATE TIttE D/tl U* TITS C 53 WD SP D Ut4ITS C SECH W T SC DI CL SP DI AIR WAT BT TURED CCttD 00 FH SAlti P 11 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 12 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 REL LS TAXA X S.E. AB%

1 2 0 LO4IDEt3TIFIED ALGAE 0.0 21088.42 10544.21 10544.21 0.2 0 UtlIDEt4TIFIED ALGAE (LPIL) 0.0 21068.42 10544.21 10544.21 0.2 0 CYAt40?HrTA 2920406.00 1729250.00 2324828.00 595578.00 47.3 0 OSCILLAT07IACEAE O 05CILLATORIA 0 OECILLAICRI A ( LPIL) 1432652.00 1729250.00 1580951.00 148299.00 32.2 0 Lit:53Y A

> 0 Lit:G3Y A I LPIL) 1487754.00 0.0 743877.00 743877.00 15.1 1 0 CHLCEOrtirTA 95918.25 42176.84 269047.50 226870.69 5.5 0 VOLVOCALES 0 *HL At )D3?iOf tAS 0 LHLAt:YCC;:OtitS I LPIL) 55102.03 21068.42 3SO95.23 17006.80 0.8 0 C::'. 000COCC A L E S O AtEISTPOD Ef tfUS 0 CONVOLuluS 55102.03 0.0 27551.02 27551.02 0.6 0 FALCATUS 165306.06 21088.42 93197.19 72103.61 1.9 0 SC E!EDE S!?JS 0 ACUMIt3ATUS 220408.12 0.0 110204.06 110204.06 2.2 0 XAMTH0Til(T A 0.0 185578.06 927S9.00 92789.00 1.9

, 0 HETEROTRICHALES g 0 TPIDCt!EtiA 0 TRIE.OttEt1A E LPIL) 0.0 185578.06 92789.00 92789.00 1.9

{ 0 CHRYS0ritrTA 110204.06 35850.32 73027.19 37176.87 1.5 3

0 0 Ct;n rSor:C?t AD tLES S 0 DIt:0'3 YC14 5 0 FOCIALE 55102.03 14761.89 34931.96 20170.07 0.7 0 0 DINOC3YC:4 (LPIL) 0.0 21083.42 10544.21 10544.21 0.2 2 0 CHP r53: 3.1.iD ALES ( LPIll 55102.03 0.0 27551.02 27551.00 0.6

{ 0 C ACILLARIOntT T A-C Et1TRIC 440816.12 231972.50 3 36 39:+ . 31 104421.81 6.8 4 0 EUTCDISCALES

1. 0 Gl0SIDA CL 0 10LitOICA 165306.06 0.0 82653.00 62653.00 1.7 7 0 EUPODI!CALES (LPIL) 275510.06 231972.50 253741.25 21708.78 - 5. 2

{ 0 D ACIL L ARIOP.41 T A-pet 3;;AT E 666734.31 2374553.00 1520643.00 853909.31 30.9 g 0 0 FRA3ItaRIAL25 ASTERIC'4ELLA 3

DATE 09/22/78 PAGE NO 1 T600 AQUA 9/28/77

NORTHEPH IICI AHA PUBLIC SERVICE COMPANY (49720)

• BAILLY GENERATING PLANT pHYTCPLA!4KTON DEttSITY HUMOER CF CELL *; PER LITER GEPLICATE REPORT LS TAXA REL X S.E. AB%

1 2 0 FORrLSA 16530.61 78027.12 0 DIATCt1A 47278.87 30748.26 1.0 0 TEt.1JE 71632.62 14761.89 0 FRAGILARIA 43197.26 28435.36 0.9 0 FRAGILARIA (LPIll 0.0 717006.31 358503.12 0 $1nECRA 358503.12 7.3 0 51HEDRA (LPILI 38571.42 778162.62 0 TALELLARIA 408367.00 369795.56 8.3 0 FEHESTRATA 0.0 253061.00 126530.50 126530.50 2.6 0 TABELLARIA (LPIL) 220408.06 284693.62 252550.8' 0 HAVICULALES 32142.78 5.1 0 HAVICULALES (LPIL) 110204.06 0.0 0 BACILLARIALES 55102.03 55102.03 1.1 0 13ITZCHIA

' [

S' 0 HITZCHIA (LPIL) 55102.03 177142.56 116122.25 61020.27 2.4 O SURIRELLALES 0 SURIRCLLA 0 SURIFELLA (LPIL) 55102.03 0.0 27F51.02 0 BACILLARIODIYTA-PEtalATE (LPILI 27551.02 0.6 99183.62 71700.62 85e42.12 13741.50 0 PIRRHOFHYTA-DINOFHYCEAE 1.7 0.0 10544.21 5272.11 5272.11. 0.1

' 0 PERIDINIALES O PERIDIllIUt1 0 PERIDIH!Ut1 ( LPIL) 0.0 10544.21 5272.11 5272.11 0 CRYPTDFitYTA 523469.19 37959.16 0.1 0 CR YPTCt'.3tCD ALES 280714.12 242755.00 5.7 0 CRIPTCitCtt45 0 CRYPT 0t10NAS (LPIL) 55102.03 37959.16 46530.59 8571.44 0 CliRCOMONtS 0.9 0 CHROOno:tAS (LPIL) 3u3061,12 0.0 151530.56 0 CRYPTOMOHADALES ( LPIL) 165306.06 151530.56 3.1 0.0 82653.00

' 0 82653.00 1.7 TOTAL 5157545.00 4668962.00 4913253.00 ig DIVERSITY (H FRIME)

DIVERSITY (J PRIME) t4 UMBER OF TAXA 3.25 0.74 21 2.89 0.69 18 3.07 0.72 27 244291.50 100.0 0.13 0.02 Af,3VE COMPUTED USIllG SAMPLE IDS g 11 12 DATE 09/22/78 PAGE HD 2 9/28/77 T600 AQUA l

m. v Q

a HCRTHERN I*CIANA PUBLIC SERVICE Car 1PAHf 849720) O j

BAILLY GE1.ERATIt4G PLANT PHfTCPLANKTON CEtiSITY ,

IturCER OF CELLS FER LITER REPLICATE REPCRT PC TC GC LOC  !

5 56 56 0 0 00 DL9ATIOtt TOW SAttP VOL Wits CUREllT TEttP l SID D ATE tit 1E D/H UNITS C SD W3 SP O LO4ITS C SECH W T SC DI CL SP OI AIR WAT BT TURDD CCtc 00 PH SALH P 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 21 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0.0 0 2.0 4 G.J 3 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 22 6/23/7S 0 0 0.0 0 0.0

- REL X S.E. AE,%

LS TAXA 1 2 0.0 94074.31 47037.16 47037.16 4.2 0 Ut4 IDENTIFIED ALGAE 47037.16 0 Ln41DENTIFIED ALGAE tLPIL) 0.0 94074.31 47037.16 4.0 720155.37 250264.87 4C5510.12 234645.25 43.6 0 C T AtEnlYT A 0 OSCILLAT09 FACE 7.E O CSCILLATCRIA 0 OSCILLATORIA (LPILS 7 0155.37 250864.87 485510.12 234645.25 43.6 0 CHLCFCGt1TA 80017.25 31353.12 556S7.63 2+329.56 5.0 p 3 VOLVCCALES 8 0 /OL%CCALES (LPILI 24A72.42 C.0 13376.21 13336.21 1.2

• O TETF ASPC*l ALE S 0 GLDEOCYST1S 0 CLOICC)STIS ELPIL) 26672.42 0.0 13336.21 13336.21 1.2 0 CHLCROOCCCA*ES 0 SCEl ECESt1US 0 SCEt;EDESt*.;S I LPIll 0.0 31358.12 15679.C6 15679.06 1.4 0 CitLO!'00CCCALES ( LPIL) 26672.42 0.0 13336.21 13336.21 1.2 0 CHRYSOniVTA 106669.59 125432.37 116C61.00 9371.34 10.4 0 CitR YSC:*.OMAD ALES O KErit tRIDie O t(Ent)RICti ( LPIL ) 53344.84 0.0 26672.42 26672.42 2.4 0 CMR TSCt".2'1AD ALES E LPIL1 53344.34 94074.31 73709.56 20364.73 6.6 h

- 0 t10tOSIGALES

• O t:0f tOSIG A 0 t1CNCSIGA (LPIll 0.0 31358.12 15679.06 15679.0f 1.4 4 0 BACILLARIOrHTTA-CEliTRIC 66681.C0 0.0 33340.50 33343.50 3.0 0 EUF031SCALEO O ttELCSIR A 0 ITLLICA 13336.21 0.0 666S.11 666S.11 0.6 -

0 EUTCDISCALLS ELPIL) 26672.42 0.0 13336.21 1333o.21 1.2

{ 0 RitI2000LEHI ALES 3

W D Rtt120*,OL HIA a O ERIEttSIS 26672.42 0.0 13336.21 13336.21 1.2 3 DACILLARIOTHfTA-FEt#1 ATE 290729.19 210099.19 250414.19 40315.00 22.5

{

• 0 FRAGILARIALES 0 DI AT0ilA 0 Tela;E 26672.42 0.0 13336.21 13336.21 1.2 3

DATE 09/22/73 PAGE NO 3 T600 AQUA 9/28/77

10GTHERN IPOIANA PUBLIC SERVICE CCitPANY (49720) O BAILLY GEt4ER ATING PLANT PHTTOPLAta(TCH CENSITY P421 DER OF CELLS PER LITER REPLICATE REPORT REL LS TAXA X 5.E. AB%

1 2 4 51NECe1 A 0 STHECRA (LPIL) 32006.90 0.0 16003.45 16003.45 1.4 0 TABELLARIA 0 FLCCCULOSA 98687.87 2195 M 8 60319.28 38368.59 5.4 0 TRAGILARIALES (LPILS 0.0 31358.12 15679.06 15679.06 1.4 0 BACILLARIALES 0 HITICHIA 0 ACICULARIS 26672.42 125432.37 76052.37 49379.9S 6s 8 4 NITZ2HIA (LPIll 0.0 31358.12 15679.06 15679.06 1.4 J BA'ILLARIon1YTA-pet #4 ATE (LPILS 306689.62 0.0 53344.81 53344.81 4.8 0 P VR2tiorHIT A-DINOPtf1CE AE 26672.42 0.0 13336.21 13336.21 1.2 0 Git CD!t41 ALES 0 Gitacolt4Itr1 y 0 Git?C0!!4ILX1 ( LPIL S 26672.42 0.0 13336.21 13336.21 1.2 m O CR1PTCPllVTA 160034.44 62716.24 111375.31 48659.10 10.0 0 CRIPTC totc0 ALES 0 Crt rintons 0 tVRSSC243 I 26672.42 0.0 13336.21 13336.21 1.2 0 CRTPTC'QNAS t LPIL 3 80017.25 0.0 40003.62 40008.62 3.6 0 RiiO000Ct4AS 0 ftItAJTA 53144.84 62716.24 58030.54 4685.70 5.2 TOTAL 1450978.00 774544.81 1112761.00 338216.56 100.0 DIW ASITY :H FRIttEl 2.94 2.84 2.91 0.C3 DIVERSITY (J FRit:El 0.70 0.87 0.79 0.03 NUTCER OF TAXA 18 10 23 A80VE cot 1FVT(3 USING SAftPLE IDS 21 22 O DATE 09/22/78 PAGE NO 4 i T600 AQUA 9/28/77 f

I 3

% p' A

NORTHERr4 ItCIAt(A PUBLIC STEVICE COMPANY (49700) o BAILLY gel 4ERATING PLANT FHYTCPLANKTott DEttSITY tOMCER CF CELLS PER LITER REPLICATE REPORT PC TC GC LOC 5 56 56 0 0 00 CURATICt4 TOW SAMP VOL WIto CtM EllT TEMP SID DATE TIME D/N Ut3ITS C 50 WO SP O t#IITS C Si - *WT SC OI CL SP DI AIR WAT DT TURBO COND 00 PH Salte P 31 6/23/73 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.. 00 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 32 6/03/78 0 0 0.0 0 0.0 0.0 C.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 REL LS TAXA X S.E. AS%

1 2 0 L:lIDEt4TIFIED ALGAE 0.0 36764.70 18330.35 18382.35 0.7 0 LetIDEtaTIFIED ALGAE t LPILI 0.0 36764.7C 16332.35 18382.35 0.7 0 CYAN 3 RHYTA 1211072.00 1507352.00 1359210.00 1431'40.00 48.2 0 O!CILLATORIACEAE O OSCILLATORIA 0 OSCILLATCRIA (LPIL) 1:11072.00 1507352.00 1359012.00 148140.00 45.2 O CHLOUCPH11A 403690.87 20:205.75 302948.31 100742.56 10.7 p 0 VCLWC 4LES a 0 CHLAW CCMotlAS N O CilL/ttt DO:* ?tAS ( LPILI 230680.50 0.0 115340.25 115340.25 4.1 0 VotVC0 ALES (LPIL) 57670.14 735:9.37 65599.75 7929.62 2.3 0 CriLOR0000CALES 0 At:KISTRCDESMUS 0 CC:4VOLUTUS 0.0 18332.35 9191.17 9191.17 0.3 0 FALCATUS 0.0 73529.37 36764.69 36764.69 1.3 0 Al2'ISTR03Est2JS E LPIL) 115340 25 0.0 57670,12 57670.12 2.0 0 CHLO.7CC00CALES ELPIL) 4.0 36764.70 18 M,' . 35 18382.35 0.7 0 CHRYSOFitYTA 173tf3.37 477940.94 3254 4.62 15:465.25 11.5 0 CliR1S0;t0!;AD ALES 0 DItCC*?YO:1

1. 0 DIVERCEttS 57670.14 36764.70 47217.41 10452.72 1.7 0 0 DIt:C:R10*4 (LPIL) 0.0 36364.70 18382.35 18330.35 0.7 0 Kent 1R10t4 0 KEritYRIC4 (LPILI 57670.14 M764.70 47:17.41 10452.72 1.7 ,

3 0 CH9Y00;LtIAD ALES ( LPIL) 57670.14 367646.87 210655.50 154933.31 7.5 l 0 BACILLARIont)TA-CLHTRIC 109573.19 64558.75 97365.94 12507.22 3.4 0 EUF03ISCALES 0 EurDDISCALES ELPIL) 103806.19 36764.70 70235.44 33500.75 2.5

= 0 R;!IZCSOLE tJI A L ES s 0 RilIZO.iOLE 11 A 3 0 ERItttSIS 5767.01 47794.10 26730.55 21013.54 0.9 g 0 B ACILLt RIOPit VT A-PENilATE 916954.94 463235.06 690095.00 226059. % 24.5

- 0 FRAGILARIALES I O DI AT0ilA 8 0 TE!!UE 46136.11 36764.70 41450.40 4625.71 1.5 k3 0 TRAGILARIA DATE 09/22/78 PAGE NO 5 T600 AQUA 9/28/77

HORTHERta ItJOIAttA PUBLIC SERVICE cot 1 pat 4Y (49720 3 0 BAILLY CENERATItG PLAT 4T PHYTCPLAt4KTOtt DEllSITY

1. 4Jt1 DER OF CELLS PER LITER REPLICATE REPORT

- REL LS TAXA X S.E. AB%

1 2 0 CROTCNEt: SIS 0.0 147058.75 73529.37 73529.37 2.6 j

0 SYt4EDRA 0 SYt4EDRA (LPIL) 92272.19 0.0 46136.09 46136.09 1.6 f l

0 TACELLARIA 0 FLCCCULOSA 599769.31 242646.94 421208.12 178561.19 14.9 0 BACILLARIALES 0 11ITZCHIA 0 ACICULARIS 115340.25 36764.70 76052.44 39287.78 2.7 0 ti!TZCHIA (LPILI 63437.14 0.0 31718.57 31718.57 1.1 0 CRYPTOrttTTA 57670.14 0.0 28835.07 2C835.07 1.0 0 CRYPT 0MCt:00 ALES 0 CR1 PTCt10t4AS 0 CRIPT0*1014AS (LPILI 57670.14 0.0 28835.07 28835.07 1.0 b TOTAL 2871969.00 2772051.00 2.49 2822010.00 2.64 49959.00 300.0 0.16 DIVERSITY (H PRIMEl 2.80 DIVERSITY (J PRIt1Z) 0.72 0.62 0.67 0.05 NUTTER CF TAXA 15 16 21 ABOVE CCt19UTED USItG SAtt?LE IDS 31 32 DATE 09/22/78 PAGE I40 6 7600 AQUA 9/28/77 8

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HORTHERil ItOIANA PUBLIC SERVICE C0t1PANY (49720 8 O

BAILLY 3Et4ERATING PLAtlT PHYTOPLAl1KTOtt DEt4SITY tRJMDER OF CELLS PER LITER STAT! Oil REPORT SMMS 1 - 3 REL LS TAXA X S.E. A8%

1 2 3 0 Ut41DEt4TIFIED ALGAE 10544.21 47037.16 18382.35 25321.24 11091.21 0.9 o U'4 IDENTIFIED ALGAE (LPIL) 10544.21 47037.16 18382.35 25301.24 11091.21 0.9 0 CTAlicoHY T A 23:4828.00 485510.12 1359212.00 1369850.00 531186.25 47.1 0 OSCILLATCRIACEAE O OSCILLATORIA 0 03CILLATORIA (LPILI 1580951.00 485510.12 1359:12.00 1141891.00 334374.50 38.7 0 LYliOSYA 0 Lit:0SY A ( LPILI 743877.00 0.0 0.0 247959.00 247959.00 8.4 0 CHLCRorHTTA 269047.50 55687.68 302948.31

• 209227.81 77391.31 7.1 0 VOLVOCALES O CH L ANY D0t'.0!4AS 0 CHLAttYD0:1Ct4AS (LPIL) 38095.23 0.0 115340.25 51145.16 33909.18 1.7 0 VOLVCCALES ( LPIL) 0.0 13336.21 65599.75 26311.98 20017.57 0.9 y 0 TETRASPC2 ALES 8 0 GLO;0 CYSTIS

• O GLOECCYSTIS (LPILI 0.0 13335.21 0.0 4445.40 4445.40 0.2 0 CHLOECCOCCALES 0 At:NISTRODES US 0 CO:lVOLUTUS 27551.02 0.0 9191.17 12:47.39 8098.76 0.4 0 FALCATUS 93197.19 0.0 36764.69 433:0.62 27102.67 1.5 0 A!!sISTRODESMUS (LPIL) 0.0 0.0 57670.12 19:23.37 19:23.37 0.7 0 SCittECESt:US 0 ACU:tIHATUS 110204.06 0.0 0.0 36734.69 36734.69 1.2 0 SCEt4EDESituS ( LPIL) 0.0 15679.06 0.0 5226.35 5206.35 0.2 0 CHLOROCOCCALES (LPIL) 0.0 13336.21 18382.35 10572.85 54S3.45 0.4 0 X AtlTHCPHY T A 92789.00 0.0 0.0 30909.66 30909.66 1.0 g 0 HETER 0TRICHALES 0 TRICO3EHA 0 TRIC0HEttA (LPIL) 92789.00 0.0 0.0 30929.66 30929.66 1.0 0 0 CHRYsopiirTA 73027.19 116061.00 325475.62 1715:1.25 77973.12 5.8 g 0 Ct!2 VS0t*0tlAD ALES 0 Dit:CCRY0t3 0 DIVEECEt4S 0.0 0.0 47217.41 15739.14 15739.14 0.5 0 SOCIALE 34931.96 0.0 0.0 11643.98 11643.98 0.4 0 DItt00RY0tl (LPILI 10544.21 0.0 18382.35 9642.1C 53 5.66 0.3 0 l* E PitYRIoll 3 0 FErHfRIOtt ( LPILI 0.0 26672.42 47217.41 246 9.95 136ES.70 0.8 g 0 Ct!AYSO:10!4ADALES (LPIL) 27551.02 73709.56 212658.50 104639.69 55608.83 3.5

=. 0 MC:OSIGALES I O HCitOSICA E O i:C'0SIGA (LPIL) 0.0 15679.06 0.0 5:06.35 5206. " 9.2 0 DACILLARIOPHfTA-CEtITRIC 336394.31 33340.50 97065.94 155600.05 90049.11 5.3 DATE 09/22/78 PAGE NO 7 T600 AQUA 9/08/77

NCRTHERN 7tCI AJLA PUBLIC SERVICE COrtPANY (49720) o B AILLY GEt ERATI'4G PLANT PHYTCPLAtKTore DENSITT tai 1EER CF CELLS PER LITER STATION REPORT STATIONS I - 3 (continued)

- REL LS T/J:A X S.E. ABX 1 2 3 0 EUPCDISCALES O ttELOSIRA 0 ITALICA 0.0 6668.11 0.0 2222.70 2222.70 0.1 0 ISLAPOICA 82653.00 0.0 0.0 27551.00 27551.00 0.9 0 EUFC0150 ALES ELPIL) 253741.25 13336.21 70285.44 112454.25 72531.12 3.8 O RHIZC00 LEt4I ALE S l 0 RHIZOSOLENIA l 0 ERIEt4 SIS 0.0 13336.21 26780.55 13372.25 7730.90 0.5 0 BACILLARICPHYTA-PENitATE 1520643.00 250414.19 690095.00 820184.06 372<.25.25 27.8 0 FRAGILARIALES l 0 ASTERICt4ELLA I O F C7ttOS A 47278.87 0.0 0.0 15759.62 15759.62 0.5 O DI AT C*tA p 0 TEffJE 43197.26 13336.21 41450.40 32661.29 9675.69 1.1 s 0 FRAGILARIA y 0 CROTCNENSIS 0.0 358503.12 0.0 0.0 73529.37 0.0 24509.79 119521.00 24509.79 119501.00 0.8 4.1 0 FR AGIL ARI A ( LPIL) 0 SYliED3A l

0 STt.ECPA (LPILI 408367.00 16003.45 46136.09 156835.50 126066.19 5.3 0 TALELLARIA 0 F EtiE STR AT A 126530.50 0.0 0.0 42176.83 42176.83 1.4 l 0.0 60319.28 421208.12 160509.12 131507.37 5.4 1 0 FLCCCULCSA l 0 TABELLARI A ( LPIll 252532.81 0.0 0.0 84183.56 84183.56 2.9 1

0 FRAGILARI ALES ( LPILI 0.0 15679.06 0.0 5226.35 5226.35 0.2 l 6 P41VICULALES 0 NAVICULALES ELPIL) 55102.03 0.0 0.0 18367.34 18367.34 0.6 5 0 BACILLAPIALES E O HITZCHIA G 0 ACICULARIS 0.0 76052.37 76052.44 50701.60 25350.80 1.7 j 0 ftITZCHIA EL"!LB 116122.25 15679.06 31718.57 54506.62 31153.81 1.8 g 0 SURIPELLALES 0 $URIRELLA g 0 EURIRELLA (LPIL8 27551.02 0.0 0.0 9183.67 9183.67 0.3 g 0 EACILLARIOPHYTA-pet l HATE ELPIL) 85442.12 53344.81 0.0 46262.31 24917.93 1.6 O P RRHOPHYTA-DIt:CPH fCE AE 5272.11 13336.21 0.0 6202.77 3877.85 0.2 0 0 Gir2.00 ZHI A LE S 0 G1t2:0DINIL28 0 Gir2:COIt:Itti ( LPIL) 0.0 13336.21 0.0 4445.40 4445.40 0.2 k 0 PEPID!NIALES 1 0 PERIDINIUM S O PERICINItti ( LPIL) 5272.11 0.0 0.0 1757.37 1757.37 0.1 0 0 CRYPTCPHfTA 280714.12 111375.31 28835.07 140308.12 7*136.31 4.8 3

DATE 09/22/78 PAGE 10 8 T600 AQUA 9/28/77

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IX)RTHERil INDIANA PUBLIC SERVICE CortPANY (49720 3

• BAILLY GENER ATIt1G PLAtiT PHYTCPLAl1KTots DENSITY PAJMDER OF CELLS PER LITER STATION REPORT STATIONS 1 - 3 (continued)

REL LS TAXA X 5.E. ABX 1 2 3 0 CRYPT r:Ot10D ALES 0 CRYPT 0;t3!AS 0 suRS50!JII 0.0 13336.21 0.0 4445.40 4445.40 0.2 0 CRYPICi;0!!AS ELPIL) 46530.59 40003.62 28835.07 30458.09 5166.75 1.3 0 RitOCO:1CttAS 0 MilluTA 0.0 58030.54 0.0 19343.51 0

19343.51 0.7 -

Cit *?CO 102:AS 0 CHROO*:Oh AS ( LPI L ) 151530.56 0.0 0.0 50510.19 50510.19 1.7 0 CRIPT0ft024ADALES (LPILI 82653.00 0.0 0.0 27551.00 27551.00 0.9 TOTAL 4913253.00 1112761.00 1822010.00 2949341.00 1093953.00 100.0 DIVERSITY (H PRIMER 3.07 2.91 2.64 2.87 0.12 DIVERSITY (J PRIME ) 0.72 0.79 0.67 0.72 0.03 f tautt3ER OF TAXA 27 23 21 44 P

ALOVE CO!!PUTED LISIttG SAMPLE IDS 11 12 21 22 31 32 DATE 09/22/78 PAGE fl0 9 T600 AQUA 9/20/77 5

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HORTHERH ItDIAHA PUBLIC SERVICE COMPANY I49720)

BA'.LLY GENER ATING PLANT PHYTOPLAt3KTON DENSITY DA21BER OF CELLS PER LITER REPLICATE REPCRT PC TC GC LOC 556560010 SAMP VOL WIND CURENT TEMP DURAT10tl TOW PH SALH P WO SP O UNITS C SECH H T SC DI CL SP DI AIR WAT BT TURDO C0tlD 00 SID DATE TIME D/H Ut4ITS C SD 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0.0 0 41 6/23/78 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 42 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 - REL X S.E. AB%

LS TAXA 1 2 11260089.0 59409:2.00 5319166.00 79.2 0 CYAHOPHTTA 621756.12 0 OSCILLATORIACEAE O OSCILLATCRIA 5019642.00 4397886.00 66.9 OSCILLATORIA (LPIL) 621756.12 9417529.00 0

0 HOSTOCACEAE O APHAt4IZGMEttoli 0.0 1842560.00 921280.00 921280.00 12.3 0 FLOS-AQUE 307093.31 180579.50 126513.75 2.4 0 CHLCROPHTTA 54065.75 7 0 CHLOROCOCCALES SCEl:EDES!RJS H 0 54065.75 0.0 27032.87 274fr.67 0.4 N 0 SCEllEDESMUS (LPIL) 0 CRUCICEHIA 0.0 204728.87 102364.44 1923o4.44 1.4 0 CUADRATA 51182.22 51102.22 0.7 CHLOPOCOCCALES (LPIL) 0.0 10:364.44 2.2 0

255911.06 161746.62 94154.44 0 CHRYSOPHYTA 67582.19 0 CHRY SO:'.O!1AD ALES 0 DItiO2,R YOH 6758.27 6754.22 0.1 13516.44 0.0 0 DIVERGEllS 13516.44 13516.44 0.2 0 SOCIALE 27032.87 0.0 13516.44 0.2 27032.87 0.0 13516.44 0 DIt40 CRYO!4 (LPIL) l g 0 kErHIRIOtt 76773.31 '*6773.31 1.0 KEPHYRICH tLPIL) 0.0 153546.62 G. O 0.0 102364.44 51102.22 51182.22 0.7 0 CHRYSOf10!MDALES ( LPIL) 6758.22 6758.22 0.1

~

0 BACILLARIOPHYTA-CENTRIC 13516.44 0.0 g 0 RHIZOSOLEHIALES 0 RHIZOSOLEHIA 6758.22 6758.22 0.1 l4 0 ERIEtiSIS 0 DACILLARIOPHYTA-PD08 ATE 13516.44 105428.12 0.0 1847676.00 9765$2.06 871123.94 13.0

' O FRAGILARIALES k 0 FRAGILARIA 153546.69 76773.31 76773.31 1.0 l 0 0

CROTottENSIS FRAGILARIA (LPIL) 3.0 0.0 890570.69 445235.31 445285.31 5.9 k 0 0

SYt:EDRA ULHA 0.0 10236.45 5118.22 5118.22 0.1 i 0.0 102364.44 51182.22 51182.22 0.7 9 0 SYt:EDRA ( LPIL) .

$ 0 TABELLARIA 3 DATE 09/22/78 PAGE NO 10 T600 AQUA 9/28/77 t.

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HORTHERN IPOIAHA PUBLIC SERVICE CottPANY (49720 3 CAILLY CENERATIt3G PLANT PHYTOPLAt4K10t4 DENSITY hut 3ER OF CELLS PER LITER REPLICATE REPCRT l

- REL LS TAXA X S.E. AB%

1 2 0 FLCCCULOSA 54065.75 542531.56 298298.62 244232.87 4.0 0 FRAGILARI ALES ( LPIL) 21626.30 0.0 10813.15 10513.15 0.1 0 BACILLAP; ALES 0 HITZCHIA 0 ACICULARIS 0.0 46064.00 23032.00 23032.00 0.3 0 HITZCHIA (LPIL) 29736.16 102364.44 66050.25 36314.14 0.9 O CRIPTOFHYTA 54065.75 409457.75 231761.75 177696.00 3.1 1 0 CRI PTOttOt:OD ALE S 0 CRYPTO:02tAS 0 ttARSSctill 27032.87 0.0 13516.44 13516.44 0.2 0 RHOOCitCtMS 0 111tRJTA 27032.87 409457.75 218245.31 191212.44 2.9 I

TOTAL 916414.37 14080222.0 7498318.00 6581903.00 100.0 f 1.83 1.28 0.05 H DIVERSITY til PRittEl 1.93

" DIVERSITY (J FRItt2 ) 0.56 0.48 0.52 0.04 tutCER OF TAXA 11 14 21 ASOVE CGttiUTED USItG sat 1PLE IDS 41 42 DATE 09/22/78 f PAGE ilo 11 )

T600 AQUA 9/28/77 5

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tCETHERN IICI ANA PUBLIC SERVICE COMPANT (49720)

BAILLY GENERATItG PLANT PitYTOPLAf0CTott DEtfSITY IAJtiBER OF CELLS PER LITER REPLICATE SEPORT PC TC GC LOC 5 56 56 0 0 10 DURATI0tt TOW sat 1P VOL WIND CURENT TEt1P SID DATE TIttE D/t4 UNITS C SD WD SP D UNITS C SECH W T SC DI CL SP DI AIR WAT BT TURBD Coto 00 PH SALf4 P 51 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 52 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 I

- REL LS TAXA X 5.2. AB%

i 1 2 l O CYANOPHfTA 7003735.00 0.0 3501867.00 3501867.00 70.8 0 OSCILLATORIACEAE l 0 OSCILLATORIA 0 OSCILLATORIA (LPIL3 802720.56 0.0 401360.25 401360.25 8.1 j 0 Lit:0DYA 0 LitC3fA ELPIL) 6040471.00 0.0 3020235.00 3020235.00 61.1 0 tCSTOCACEAE p 0 APH/ HIZO* TEN 0t n 80272.06 I e 0 FLOS-AQUL 160544.12 0.0 80272.C6 1.6

• 120408.06 734140.75 427274.37 306366.31 8.6 l

j 7 0 CHLOPOPHYTA 0 VOLVOCALES

)

i 0 VOLVOCALES (LPIL) 0.0 27393.31 13696.66 13696.66 0.3 O CHLOROCOCCALES )'

0 AIEISTRODES!US l 0 FALCATUS 60204.05 109573.25 84888.62 24684.60 1.7 j 0 00 CYSTIS 0 00CVSTIS ILPIL) 602 % .05 0.0 30102.02 30102.02 0.6 l 0 SCEt3EDEStUS 0 SCEt:ECE0tU3 (LPIL) 0.0 49307.96 24653.96 24653.9S 0.5 0 CHLCROCOCCALES tLPILI 0.0 54786.62 27393.31 27393.31 0.6 0 ULOTRICHA;ES g 0 ULOTRICHALES (LPIL) 0.0 493079.62 246539.81 246539.81 5.0 0 CHRYSOPHTTA 28095.22 273933.12 151014.11 122918. % 3.1 0 CHRISO:10t3ADALES 0 DII;CERYCH 0 DIVERGENS 0.0 27393.31 13696.66 13696.66 0.3 0 SOCIALE 8027.20 54786.62 31406.91 23379.71 0.6 1 0 0

EEPHIRICf4 KEPH1RI0tt ;LPIL) 0.0 27393.31 13696.66 13696.66 0.3 0 a canySenet:ADALES (LPIL) 20068.02 164359.87 92213. % 72145.87 1.9 I

0 EACILLARIOFHTTA-CEtiTRIC 60204.05 0.0 30102.02 30102.02 0.6 h

O EUPODISCALES E O EUPCDISCALES ILPIL) 60204.05 0.0 30102.02 30102.02 0.6 k 0 BACILLARIOTHYTA-pet #4 ATE 12422C9.00 287629.69 764919.31 477289.62 15.5 1 0 FRAGILARIALES 0 0 ASTERI0t3ELLA 3

DATE 09/22/78 PAGE NO 17 T600 AQUA 9/2S/7.

8

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NORTHERN It01ANA PUBLIC SERVICE COMPANY (497203 BAILLY GENERATING PLANT PHTTCPLANKTON DENSITY PAX-SER OF CELLS PER LITER REPLICATE REPORT ret LS TAXA X S.E. AS%

1 2 0 foi':054 16054.*1 0.0 8027.20 8027.20 0.2 0 DIATOilA 0 TENUE 30102.00 0.0 15051.00 15051.00 0.3 0 FRAGILARIA 0 FRAGIL ADIA ( LPIL) 732482.44 0.0 366241.19 366241.19 7.4 0 SINEDRA 0 SIN 1 ORA (LPill 16054.41 13696.65 14S75.53 1178.88 0.3 0 TAEELLARIA 0 FEtlESTRATA 118401.25 0.0 59200.62 59200.62 1.2 0 FLOCCULOSA 0.0 189013.81 94506.37 94506.87 1.9 0 TACILLARIA ILPILI 262893.94 0.0 131445.44 131445.44 2.7 0 FRAGILARIALES tLPILI 6020.40 0.0 3010.20 3010.20 0.1 0 stav1CULALES

> O H%VICULALES (LPIll 40136.03 0.0 20068.01 20C68.01 0.6 l h" u

0 E,ACIL L AR T A LE S 4 141TZCilI A 0 ACICULARIS 0.0 30132.64 15066.32 15066.32 0.3 0 CACILLARIOF:tTT A-PDRIATE ( LPILI 20068.02 54786.62 37427.32 17359.30 0.8 0 CRIPicntrTA 80272.06 54786.62 67529.31 0 CR f P TCMO*200 ALE S 12742.72 1.4 0 E bCCOt*2:4AS 0 MIt4UTA 0.0 54786.62 27393.31 27393.31 0.6 0 CHR002:0!!AS 0 CilRCOnat1AS ( LPIll 80272.06 0.0 40136.03 40136.03 0.8 3 TOTAL 8534920.00 1350487.00 4942703.00 3592216.00 100.0 0 DIVERSITY (H FRIMEl 1.70 3.05 2.37 0.68 DIVERSITY (J FRIME) 0.41 0.80 0.60 0.20 NU::SER OF TAXA 18 14 27 0

0 AE.QVE CCMPUTED USIllG SAMPLE IDS 51 52 '

1 DATE 09/22/78 0 PAGE HD 13 O T600AGUA 7/28/77 a

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3

NORTHERN ItCIANA PUBLIC SERVICE cot 1PANY (49720) o BAILLY GEt4ERATING PLANT PHYTOPLAtETOrt DENSITY f1Jt1 DER OF CELLS PER LITER REPLICATE REPORT PC TC GC LOC 5 56 56 0 0 10 OURATION TOW SAMP VOL WIND CUR EtIT TEttP SID DATE f!!tE D/H ut!TS C SD W3 SP D uiITS C SECH H T SC DI CL SP DI AIR WAT BT TURCD C0tc CO PH SALN P 61 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 62 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0

- REL LS TAXA X S.E. AB%

1 2 0 ulIDENTIFIED ALGAE 0.0 80017.25 40008.62 40008.62 0.5 0 uilDENTIFIED ALGAE ( LPILI 0.0 80017.25 40008.62 40008.62 0.5 0 CY At30PliY T A 342416.37 8068402.00 4205409.00 3862992.00 48.5 0 OSCILLATOR!ACEAE O OSCILLATORIA 0 OSCILLATCRIA (LPILD 0.0 3480750.00 1740375.00 1740375.00 20.1 0 CSCILLATCRIACEAE (LPIL) 144175.31 0.0 72037.62 72087.62 0.8

> 0 HOSTCCACEAE

[ 0 Att1D AEH A o O At4ADAErtA (LPIll 0.0 80017.25 40008.62 40008.62 0.5 0 ArilA14IZOttEH0H 0 FLOS-AGUE 198241.06 4507635.00 2352938.00 2154696.00 27.1 0 CHLOROP;lYTA 504613.62 32006.90 268310.25 236303.31 3.1 0 VOLVOCALES 0 VOLVOCALES (LPIL) 36043.83 26672.42 31358.12 4685.70 0.4 0 Cl!LORCCOCCALES 0 At*IST R00 E".;1US 0 FALCATUS 0.0 5334.48 2667.24 2667.24 0.0 0 MICRtCTINIUM 0 t1ICR ACTINIUt1 ( LPIL) 468569.81 0.0 234284.87 234284.87 27 l 0 XANTH 0rH5TA 0.0 40008.63 20004.32 20004.32 0.2 0 HETERO 1RICHALES 0 TRIDOH!ttA 0 TRIEOt4EttA (LPIL) 0.0 40008.63 20004.32 20004.32 0.2 0 0 CHRYSOPHYTA 360435.!: 320068.94 340253.50 20184.59 3.9 0 CitR YS0t1C*LtD ALES i( 0 0

0 DItCCRYON DIVERGENS SOCIALE 0.0 0.0 240051.75 26672.42 120025.87 13336.21 120025.87 13336.21 1.4 0.2 9 0 KEntVRICH

1. 0 KEPHYRI0t4 (LPILI 36043.83 0.0 18021.91 10021.91 0.2

& O sHRYSCt10H AD ALES ( LPIL I 324394.31 0.0 162197.12 162197.12 1.9

{ 0 CHRYSOPHYTA (LPIL) 0.0 53344.84 26672.42 26672.42 0.3 0 BACILLARIOPHYTA-CENTRIC 0.0 26672.42 13336.21 13336.21 0.2 0 EUPODISCALES g 0 EUPCDISCALES (LPIL) 0.0 26672.42 13336.21 13336.21 0.2 DATE 09/22/78 PAGE HQ 14 T600 AQUA 9/28/77 C G Y

x HOQiHERN INDIANA PUBLIC SERVICE COMPAt4Y (497209 BAILLY GENERATIt4G PLANT PHYTOPLAliKTCal DEllSITY IPA:BER OF CELLS PER LITER REPLICATE REPORT

- REL X S.E. AB:{

LS TAXA 1 2 0 BACILLARIOPHYTA-pet #4 ATE 266724.25 7260222.00 3763473.00 3496748.t3 43.4 0 FRAGILARIALES O DIATO!1A 3 TENUE 0.0 122693.06 61346.53 61346.53 0.7 0 F R AGILI.RI A 0 CROT014ENSIS 0.0 826845.00 413 22.50 413e22.50 4.8 0 FRAGILARIA ILPILI 0.0 42675S4.00 2133792.00 2133792.00 24.6 0 S YtaEDR A 0 SYte!CRA 4LPILI 43252.60 8001.73 25627.16 17625.43 0.3 0 TABELLARIA 0 FLOCCULOSA 194636.62 1595006.00 894321.31 700184.69 10.3 0 FRAGILARIALES (LPILI 0.0 373413.87 186706.94 186706.94 2.2 0 NAVICULALES

(

e 0

0 HAVICUtALES (LPIL)

BACILLARIALES 0.0 26672.42 13336.21 13336.21 0.2

" O HITZCHIA 0 ACICULARIS 28535.06 13336.21 21035.64 7749.43 0.2 0 ttITZCHIA ELPIL) 0.0 26672.42 13336.21 13336.21 0.2 0 F VNiicFHYT A-DINOF111CE AE 3604.33 0.0 1802.19 1802.19 0.0 0 PERIDIll! ALES 0 PERIDIllIUM 0 IttCONSPICUUt1 3604.3S 0.0 1802.19 1802.19 0.0 0 CRYPTOFHYTA 36043.83 0.0 16021.91 18021.91 0.2 0 CRY PTOTil30 ALES 0 CRIPTCT:Cil AS 0 CRIPT0!!3HAS (LPILI 36043.83 0.0 18021.91 18021.91 0.2 TOTAL 1513833.00 15827391.0 8670612.00 7156779.00 100.0 DIVERSITY (H PRIMEl 2.75 2.56 2.65 0.10 DIVERSITY (J PRIME) 0.79 0.59 0.69 0.10 iM DER OF TAXA 11 20 26 ACOVE CCitPUTED USIt4G SAttPLE IDS 61 62 O DATE 09/22/13 8 PAGE NO 15 9/28/77

$ T600 AQUA 1

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REL LS TAXA X S.E. ABX 4 5 6 0 KEPHYRIOtt iLPIL) 76773.31 13696.66 18021.91 36163.96 20343.03 0.5 0 CHRYS0tonD ALES (LPIL) 51182.22 92213.94 162197.12 101864.37 32403.46 3.4 l 0 CHRYSOPitYTA (LPIL) 0.0 0.0 26672.42 8590.80 8890.80 0.1 l

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= 0 PERIDIttIALES 0 0 r:RIorr:Itc1 l 0 It:CCt13PICUL11 0 CRYPTOPHYTA 0.0 231761.75 0.0 67529.31 2E02.19 16021.91 600.73 105770.94 600.73 64596.18 0.0 1.5 E O CR) P10f:Ot:0C ALES k 0 CRIPTOtDIAS 1 0 ttARSSONII 13516.44 0.0 0.0 4505.48 4505.48 0.1 0 0 CRIPTot:3t:AS (LPILI 0.0 0.0 18021.91 6007.30 6007.30 0.1 2

DATE 09/22/78 PAGE F0 17 T600 AQUA 9/28/77

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1 2 0 CYAt:0PHTTA 2191464.00 7941175.00 5066319.00 2874855.00 39.8 0 OSCILLATORIACEAE O OSCILLATORIA 0 OSCILLATCRIA (LPIL) 2191464.00 196C:784. 00 2076124.00 115340.00 16.3 0 NOSTOCACEAE O APH1JIIZOMEt30t4 0 FLOS-ACUE 0.0 5930391.00 2990195.00 2990195.00 23.5 0 ClitonoritYTA 57670.14 392156.87 224913.50 167243.31 1.8 f 0 1EIRASr. MALES N 0 ELAKATOTHRIX

• 57670.14 O ELAKATOTt: RIM (LPIL) 0.0 23335.07 28335.07 0.2 0 CHLORCCCCCALES C SCEtiECES13S 0 ACLtt1HATUS 0.0 392156.87 196078.44 196073.44 1.5 0 CHRYSOFHYTA 207612.44 539215.69 373414.06 165601.62 2.9 0 CHrtiSOMO*4 AD AL ES 0 OIt203R (C'1 0 SOCIALE 92272.19 0.0 46136.09 46136.09 0.4  ;

O MCt40$1GLLES 0 t'.Ot:0SIGA 3 0 t1C*tOSIGA (LPIL) 115340.25 539015.69 327277.94 211937.69 2.6 0 0 BACILLAR 1orHYTA-CENTRIC 426758.94 49019.61 237839.25 188869.62 1.9 0 EUF00! SCALES 0 SKE LETC'IEt1A g 0 POTAt:US 374855.87 0.0 187407.94 187427.94 1.5 0 RHIZCSOLEt41 ALES 0 RHIZDSOLFHIA i

I 0 ERIEt3 SIS 0 B ACILL ARIOrltV T A-PEtN ATE O FPAGILARIALES 51903.12 5397721.00 49019.61 8166659.00 50461.37 67S2290.00 1441.75 0.4 1384369.00 53.3 O O DI A10M4 0 TEttJE 1926182.00 137254.81 1031718.37 894463.56 8.1 E O FRLGILARIA

$ 0 CRCTO4CNSIS 317185.75 9803.92 163494.81 153690.87 1.3 3 0 TRACILARI A (LPIL) 2631660.00 6558233.00 4634946.00 1953286.00 36.4

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CATE 09/22/78 PAGE HQ 27 T600 AQUA 9/28/77

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BAILLY GEHERATING PLANT FHTTOPLANKTON DENSITY tAJt1BER OF CELLS PER LITER REPLICATE REPCRT PC TC GC LOC 5 56 56 0 1 00 DURATI0tt TOW SAf1P VOL WIND CURENT TEt1P SID DATE TIttE D/H Uf4ITS C SD W3 SP D utITS C SECH W T SC 01 CL SP OI AIR WAT BT TURED COND DD PH SALH P 81 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 82 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0

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REL LS TAXA X S.E. AB%

1 2 0 ulIDEtiTIFIED ALGAE 77s140.19 3'115.65 406127.87 367012.25 10.4 0 UNIDENTIFIED ALGAE (LPIL) 773140.19 39115.65 406127.87 367012.25 10.4 0 CYAl;0FHTTA 8434 5.69 2659263.00 1751644.00 908:18.62 45.1 0 OSCILLATORIACEAE O OSCILLATORIA 0 OSCILL ATORIA (LPIL) 843425.69 2659563.00 1751644.00 90S218.62 45.1 0 CHLOR 0rHVTA 28114.19 234693.81 131404.C0 103:09.75 3.4 0 VOLVOCALES y 0 CHL Arn00tt0H A S N 0 CHLM1YCOMCl4AS (LPIL) 22114.19 0.0 14057.09 14057.09 0.4 W O CHLCRCCCCCALES 0 ANKISTRODESt!US 0 F A LC ATUS 0.0 78 31.25 39115.62 35115.62 1.0 0 SCEt:EDESiluS 0 ACUMIt!ATUS 0.0 156462.56 78231.25 78231.25 2.0 0 XANTHCFHYTA 112456.75 0.0 56228.37 562 8.37 1.4 0 RHIZCCHLC21 DALES O STIPITOCOCCUS 0 STIPITOCCCCUS (LPIL) 112456.75 0.0 56208.37 56228.37 1.4 0 Cha1SOPHYTA 295198.94 89965.94 19:582.44 102616.50 5.0 g 0 CHR fSCnot4ADALES O O DIHOBRIC'4 0 SERTULARI A f* . 0 11734.69 Fe67.34 5867.34 0.2 0 DIVERGENS 28114.19 0.0 14057.09 14057.09 0.4 g 0 SOCIALE 14057.09 0.0 7028.55 7028.55 0.2 0 CHR150:tCt4ADALES (LPIL) 253007.69 78231.25 165629.44 87393.19 4.3 0 BACILLARICPHYTA-CENTRIC 11245.63 195578.12 103411.87 9:166.19 2.7 0 EUFDDISCALES 0 EUPCDISCALES (LPIL) 0.0 195578.12 97789.06 97789.06 2.5 E D RHIzosotEHIAtES O O RdIZOSOLENIA O O EPIENSIS 11245.68 0.0 5622.84 5622.84 0.1 6 0 DACILLARIDFHYTA-pet #3 ATE 272707.56 496768.50 3833.00 112030.44 9.9 1 0 FRAGILARIALES ASTERIO!;ELLA S 0

$ 0 FCRMOSA 95538.19 46938.77 71063.44 24324.71 1.8 3

DATE 09/22/78 PAGE NO 29 T600 AQUA 9/28/77

HORTHERH ItCIANA PUBLIC SERVICE COMPANY (49720 3 O BAILLY GENER ATING PLANT PHITOPLANKTON DENSITY MEEER OF CELLS PER LITER REPLICATC REPORT

- REL X 'S.E. AS%

LS TAXA 1 2 0 DIATortA 0 TEtUE 5622.84 0.0 2811.4* 2811.42 0.1 0 SYt:ECR A 0 SYt4EORA (LPILI 0.0 39115.65 19557.82 19557.82 0.5 0 TASELLARIA 0 FLOCCULOSA 143382.37 265986.31 204684.31 61301.97 5.3 0 BACILLARIALES 0 ti!TZCHIA 0 ACICULARIS 28114.19 0.0 14057.09 14057.09 0.4 0 NITZCHIA (LPIL) 0.0 66496.56 33248.28 33248.28 0.9 0 C ACILLARIOFHYTA-PEtNATE ( LPIL) 0.0 78231.25 39115.62 39115.62 1.0 0 CRIPicalT TA 146193.75 1576360.00 861276.87 715083.12 22.2 O CRYPTor10r200 ALES 0 RHOOO10ftAS 146193.75 154462.56 151328.12 5134.41 3.9 I O F11tAJTA N O CYAttotCHAS

" 0 2YA!;0!!Ct4AS (LPIL) 0.0 39115.65 19557.82 19557.82 0.5 I O CRYPT 0r10t1ADALES (LPIL) 0.0 1380782.00 690391.00 690391.00 17.8 TOTAL 2482479.00 5292335.00 38S7407.00 1404923.00 100.0 2.57 2.28 2.43 0.14 DIVERSITY (H FRIME)

DIVERSITT (J PRINE) 0.69 0.58 0.64 0.05 tAJOER OF TAXA 13 15 22 ABOVE COMPUTED US!!!3 Sat 1PLE IDS 81 82 DATE 09/22/78 PAGE HD 30 T600A7JA 9/28/77 e

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HORTHERN INDI ANA PUBLIC SERVICE COMPANY (49720)

O BAILLY CEt;ERATING PL ANT PHYTOPLAtlK1CH DENSITY HUM 3ER Of CELLS PER LITER REPLICATE REPORT FC TC CC LOC 5 56 56 0 1 00 OURATICil TOW SAMP VOL WIND CURENT TEMP

$10 DATE TIME O/H UNITS C SD HD SP D UNITS C SECH W T SC 01 CL SP DI AIR HAT DT TURBO CO!!D DO PH SALH P 91 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 92 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0

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DII;C3R1011 OIVEDGEtts 0.0 43252.60 21626.30 21626.30 0.3 h 0 CYLINCRICUM 40136.05 0.0 20063.02 20068.02 0.3 0 SOCIALE 0.0 86505.19 43252.59 43252.59 0.6 0 0 110tl0SIG ALES

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1. 0 t*Dt:0SIGA ( LPIL) 0.0 21626.30 10313.15 10813.15 0.1 lU 0 DACILLARIOPHYTA-CEt4TRIC 662244.81 112456.69 387350.75 274894.06 5.0 3

DATE 09/22/78 PAGE NO 31 T600 AQUA 9/28/77

NORTHERif IPOIANA PUBLIC SERVICE COr'.PANY (49720)

BAILLY GENERATItG PLANT PHYTOPLAfETCH OENSITY t&#12ER OF CELLS PER LITER REPLICATE REPCRT

- REL LS TAXA X S.E. ABZ 1 2 0 EUPODISCALES 0 ftE LOSIR A 0 ISLAt: DICA 0.0 54065.75 27032.27 27032.87 0.3 0 EUPODISCALES ILPIL) 602040.75 43 52.60 322646.62 279394.06 4.1 0 RHIZOSOLEHIALES 0 RHIZCEOLEHIA 0 ERIENSIS 60204.07 15138.41 37671.04 2:532.83 0.5 0 BACILLARICPHYTA-pet 44 ATE 622108.75 415224.69 513666.69 103442.00 6.7 0 FRAGILARIALES 0 ASTER 10t1ELLA 0 FORitOSA 250850.31 112456.69 181653.50 69196.81 2.3 0 FRAGILARIA 0 CROTCt!ENSIS 250550.31 0.0 1254:5.12 1 5425.12 1.6 0 FRAGIL ARIA (LPIL) 0.0 6457.89 3:43.94 3:43.94 0.0 7

ha 0

0 Sit 4ECRA ULtlA 0.0 8650.52 4325.06 43 5.26 0.1

• O SYNEDRA (LPIL) 0.0 12975.78 6487.89 64S7.89 0.1 0 TABELLARIA 0 FLOCCULOS4 0.0 253027.62 16513.81 126513.81 1.6 0 t4AVICULALES 0 NAVICULALES (LPIL) 70238.06 0.0 35119.03 35119.03 0.5 0 BACILLARIALES 0 HITZCHIA '

0 t11TZCHIA (LPIll 50170.06 0.0 25035.03 25085.03 0.3 0 BACILLARIOPHYTA-pet # TATE ILPIL) 0.0 21626.30 10813.15 10313.15 C.1 0 CRYPTCPHYTA 250350.31 151334.06 201117.19 49733.12 2.6 g 0 sRIPTCMCf10 DALES O O CR Y PTCitCil AS 0 CRYPTO 10t1AS (LPILI 0.0 43252.60 216 6.30 21626.30 0.3 0 RMCDC*t:14AS 0 ftIIPJTA 0.0 6487S.90 32439.45 32439.45 0.4 0 LENS 0.0 43252.60 21606.30 21606.30 0.3 0 CRYPTOttotlADALES (LPIll 250850.31 0.0 1:5425.12 125425.12 1.6 4 TOTAL 10455435.0 5108120.00 7781777.00 2673657.00 100.0 I DIVERSITY (H FRIttEl 2.54 1.75 2.15 0.40 0 DIVERSITY (J FRIttEl 0.69 0.40 0.54 0.14 O BAX1EER CF TAXA 13 21 30 I ABOVE COrtPUTED USING sat 1PLE IDS 91 9 3 DATE 09/02/78 PAGE NO 32 T600 AQUA 9/28/77

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HO2THERH INDIANA PUBLIC SERVICE CO*1 PANT (49720)

D AILLY GEt!! RATING PLANT FHYTOPLANKTOff DENSITT t!UtSER CF CELTS PER LITER STATICil REPO7T STATIONS 7 - 9 LS TAXA REL X S.E. AS%

7 8 9 0 Ut3 IDENTIFIED ALGAE 0.0 406127.87 183823.50 0 UtlIDENT!FIED ALGAE iLPIL) 196650.44 117414.25 2.4 0 CYAt;OFHTTA 0.0 406127.87 183323.50 196050.44 117414.25 5066319.00 2.4 1751644.00 5769666.00 4195876.00 1238867.00 51.6 0 CHROCCCCCACEAE O t*ICRCCYSTIS 0 NICRCCVSTIS (LPIL) 0 0.0 0.0 702380.50 23t126.f.1 234126.81 2.9 OSCILLATORIACEAE O CSCILLATORIA 0 OSCILLATCRIA (LPIL) 2076124.00 1751644.00 4063884.00 2630550.00 0 lyt;G314 722762.06 32.3 0 Lit 3GSVA (LPIL) 0.0 0.0 10U3401.00 33 % 67.00 334467.00 4.1 0 t0STOCACEAE O APH A14IZC:1DCil 0 FLCS-AQUE 2990195.00 0.0 0.0 996731.62 I O CHLCF0Fil(TA 224913.50 131404.00 589716.50 996731.62 12.3 N O VCLYCCALES 315344.62 139316.44 3.9 O CHLAtlT00:lottAS 0 CHLAthDCitO'nS ( LPIL) 0.0 14057.09 0.0 4685.70 4635.70 0 TETRASFCDALES 0.1 0 E L AK ATOTHRIX 0 ELAMATOTH21X tLPIll 28835.07 0.0 0.0 9611.69 0 CHLCROCOCCALES 9611.69 0.1 0 AttKISTRCDESt:US 0 FALCATUS 0.0 39115.62 5406.57 14840.73 12237.33 0 GOL EtElttI A 0.2 0 COLEtMIt4IA (LPIL) 0.0 0.0 10313.15 g 0 SCEt:ECES:fJS 3604.33 3604.33 0.0 w 0 ACUt11NATUS 196078.44 78231.25 0.0 91436.56 0 SCEt:ECESt10S (LPIL) 0.0 56936.76 1.1 0

0.0 21626.30 72C8.77 7203.77 0.1 TETRAEDRO:4 0 iUTICUt1 0.0 0.0 50170.06 0 CHLCRCCCCCALES (LPIL) 16723.35 16723.35 0.2 0.0 0.0 501700.62 0 XAt47tf0PHYTA 167233.50 167233.50 2.1 0.0 56228.37 35683.37 30637.25 16426.65 0.4 1 0 0

RHI2CCHLC2IDALES STIPITCCOCCUS I 0 STIPITOCOCCUS tLPIL) 0.0 56228.37 35683.37 l 0 CHR YS0!HY TA 0 CitRYSottottADALES 373414.06 192582.44 95760.00 30637.2$

220535.50 16426.65 81365.56 0.4 2.7

& 0 OIl:CERICil

{ 0 SERTULARIA 0.0 5867.34 0.0 1955.78 1955.78 0 DIVERGENS 0.0 0.0 14057.09 21626.30 11894.46 0 CV LItDRittri 0.0 6335.93 0.1 0.0 20068.02 66S9.34 66C9.3. 0.1 OATE 09/22/78 PAGE NO 33 T600 AQUA 9/28/77

NORTHERN 1}CIANA PUBLIC SERVICE CortPANT (49720 3 BAILLY CENERATItG PLANT PH1TCPJNKTON DD4SITY PAEBER OF CELLS PER LI'ER STATION REPORT STATIONS 7 - 9 (continued)

- REL LS TAXA X S.E. AS%

7 8 9 0 SOCIALE 46136.09 7028.55 43252.59 32139.08 12582.83 0.4 0 CHRISOt10t4ADALES (LPIL) 0.0 IG5629.44 0.0 55209.81 55209.81 0.7 6 0 #10tCSIGALES 0 t'.OtCSIG A 0 tO CSIGA (LPIll 327277.94 0.0 10813.15 112697.00 107335.81 1.4 0 BACILLARICPHTTA-CENTRIC 237t S9.25 103411.87 357350.75 242883.94 820C4.12 3.0 0 EUPODISCALES 0 t1E LOSIR A 0 ISLAtCICA 0.0 0.0 27032.87 , 9010.96 9010.96 0.1 0 SKE LE TCt:EttA 0 POTAfUS 187427.94 0.0 0.0 62475.98 62475.93 0.8 0 EUPODISCALES ( LPIL) 0.0 97789.06 322646.62 140145.19 95517.44 1.7 0 RHI2050LENIALES 0 RHI2CSOLENIA

> 0 ERIEttSIS 50461.37 5622.84 37671.24 31251.81 13335.79 0.4 da O BACILLARIOFHYTA-PENNATE 6782290.00 384738.00 518666.69 2561893.00 2110550.00 31.5 CD 0 FRAGILA91 ALES 0 ASTERIOt4ELLA 0 FCPt10SA 0.0 71263.44 181653.50 843C5.62 52842.76 1.0 ,

O DIATOttA 0 TEtCE 1031718.37 2811.42 0.0 344843.25 343438.50 4.2 0 FRAGILARIA 0 CRO10tilliSIS 163494.81 0.0 125425.12 963C6.62 49391.47 1.2 0 FRAGILAkIA ( LPILI 4634946.00 0.0 3243.94 1546063.00 1544441.00 19.0 0 Sit:ECRA 0 ULHA 0.0 0.0 4325.26 1441.75 1441.75 0.0 0 Sit 4ED94 (LPIl l 42675.89 19557.82 6487.89 22907.20 10579.% 0.3 h 0 TAEELLARIA 0 FLOCCULOSA 7696C6.19 204684.31 126513.81 366934.75 202596.31 4.5 0 NAVICULALES 0 NAVICULALES (LPIL) 0.0 0.0 35119.03 117C6.34 11706.34 0.1 0 0 BACILLARIALES 0 t1IT2 CHIA i[ 0 O

ACICULARIS HIT 2 CHIA (LPIll 0 BACILLARIOFHITA-PUNATE (LPILI 28835.07 53344.87 57670.12 14057.09 33248.2S 39115.62 0.0 25085.03 10813.15 14297.39 37226.06 35856.30 6324.83 83 % .86 13623.66 0.2 0.5 0.4 0 CRIPTOPHTTA 49019.59 261276.87 201117.19 37C471.19 249299.69 4.6 3 0 CRI P tot'.O!CD ALE S

& O CRI PTOt:Ott AS 0 CRYPTCitOHAS (LPILI 24509.80 0.0 21626.30 15378.70 7734.27 0.2

{

g 0 RHO 30tONAS 0 tiltUTA 24509.80 151328.12 32439.45 69425.75 41015.09 0.9 DATE 09/22/78 PAGE HO 34 T600 AQUA 9/28/77 k q -)

NCRTHERei It0!ANA PUBLIC SERVICE CottPAtli (49720 8 0 BAILLY GEtIRATING PLAtti PHYTOPLAf!KTCH OENSITY FAA10ER OF CELLS PER LITER STATION REPORT STATIONS 7 - 9 (continued)

REL LS TAXA X S.E. AS%

7 8 9 0 LENS 0.0 0.0 21626.30 7208.77 CYAt;Ct10t4AS 7208.77 0.1 0

0 CVAt: Ort 2t4AS (LPIL) 0.0 19557.82 0.0 6519.27 6519.27 0.1 0 CRYPT 0t.CNADALES ELPILI 0.0 690391.00 125425.12 271938.69 212335.87 3.3 TOTAL 12733840.0 3887407.00 7781777.00 8134341.00 2559822.00 100.0 DIVERSITY EH PRIMES 2.34 2.43 2.15 2.?0 0.08 01VERSITY (J PRIttEl 0.62 0.64 0.54 0.60 0.03 tR#%ER OF TAXA 18 22 30 40 ASOVE CCitPUTED USittG SAttPLE IDS 71 72 81 82 91 92 I

r2 DATE 09/22/78

• PAGE NO 35 T600 AQUA 9/28/77 8

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HORTHERN ItOIANA PUBLIC SERVICE COMPANT (497203 O

BAILLY GENERATIliG PLAtiT PHTTOPLAIKTON DENSITY HUMBER OF CELLS PER LITER REPLICATE REPORT PC TC GC LOC 556560020 DURATION TOW SAf1P VOL WIND CURENT TEMP SID DATE TIME D/t4 UNITS C SD WD SP D UNITS C SECH H T SC DI CL SP DI AIR WAT BT TURBD COND DO PH SALN P 101 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 102 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 REL LS TAXA X 5.E. AB%

1 2 0 UNIDENTIFIED ALGAE 0.0 465686.31 232643.12 232843.12 7.3 0 UtlIDENTIFIED ALGAE ELPILI 0.0 465686.31 232843.12 232843.12 7.3 0 CYAt:0PHYTA 0.0 4341838.00 2170919.00 2170919.00 68.3 0 OSCILLATORIACEAE O OSCILLATCRIA 0 OSCILLATORIA (LPILI 0.0 4341838.00 2170919.00 2170919.00 68.3 0 CHLORortiVTA 30997.70 260236.44 145617.06 114619.31 4.6 0 VOLVCCALCS f 0 CH LA!11 DCt'.0ttAS u 0 CHLAM100' tot 1AS t LPIL) 0.0 27393.31 13696.66 13696.66 0.4 0 0 VOLVOCALES (LPIL? 0.0 27393.31 13696.66 13696.66 0.4 0 CHLOROCOCCALES 0 DICTYOSPILAERIU'1 0 DICTYOSPHAERIUH ( LPILI 0.0 27393.31 13696.66 13696.66 0.4 0 CHLOROCOCCALES (LPIL) 30997.70 109573.25 70285.44 39287.78 2.2 0 ULOTRICHALES 0 ULOTHRIX 0 ULOTHRIX (LPIL) 0.0 68483.25 34241.62 34241.62 1.1 0 CHRYSOPHYTA 154988.44 164359.87 159674.12 4685.72 5.0 0 CHR YS0t10tIAD ALES g 0 DIriOBR10N O 0 OIVERGEtts 0.0 82179.94 41089.97 41089.97 1.3 0 kEFH) RIOT 1 0 KEPHYRICH (LPIL) 30997.70 0.0 15498.85 15498.85 0.5 0 tilRYSOCHRot1ULINA ,

O PARVA 0.0 82179.94 41089.97 41089.97 1.3 0 CHRYS0ttoriADALES ( LPIL) 123990.75 0.0 61995.37 61995.37 2.0 1 0 BACILLARIOFHYTA-CENTRIT.

0 EUP00! SCALES 27897.92 32871.97 30384.9h 2487.02 1.0 0 EUPODISCALES (LPIL) 0.0 32871.97 16435.98 16435.98 0.5 0 RHIZ 000LEt4IALES 0 RHIZDSOLEHIA

& 0 ERIEt: SIS 27897.92 0.0 13948.96 13948.96 0.4 k

g 0 BACILLARIOFHYTA-PEtOIATE 0 FRAGILARIALES 195265.37 454728.81 325007.06 129721.69 10.2 O 0 DIAronA 3

DATE 09/22/76 PAGE NO 19 T600 AQUA 9/28/77

% Q A

NORTHERH IIDIAHA PUBLIC SERVICE COMPANY (49720)

O BAILLY GENERATING PLAT 4 PHTTOPLANKTOH DENSITY HUFOER OF CELLS PER LITER REPLICATI DEPORT REL LS T4 X S.E. AB%

1 2 0 it UE 0.0 16435.98 8217.99 8217.99 0.3 0 FRAG'LARIA 0 C c. TONEHSIS 24798.16 0.0 12399.08 12399.08 0.4 0 Sit:EL s 0 SYNEDE 6 (LPIL) 30997.70 73961.94 52479.82 21482.12 1.7 0 TABELLARIA 0 FLOCCULOSA 133290.06 142445.19 137067.62 4577.56 4.3 0 BACILLARIALES 0 N11ZCHIA 0 ACICULARIS 0.0 106833.87 53416.94 53416.94 1.7 0 HITZCHIA (LPIL) 6199.54 87658.56 46929.05 40729.51 1.5 0 CACILLARIOPHTTA-pet #4 ATE (LPIL) 0.0 27393.31 13696.66 13696.66 0.4 0 CRYPIOPHYTA 61995.39 164359.87 113177.62 51182.24 3.6

> 0 CRIPTO:10tJOD ALES 8 0 RHODO:OHAS N 'O MINUTA 61995.39 136966.56 99450.94 37485.59 3.1 0 LEtiS 0.0 27393.31 13696.66 13696.66 0.4 TOTAL 471164.75 5884066.00 3177615.00 2706450.00 100.0 DIVERSITY (H PRIME) 2.73 1.74 2.24 0.49 DIVERSITY (J PRIt1El 0.86 0.42 0.64 0.22 taltOER OF TAXA 9 . 18 22 ACOVE CO".PUTED USING sat 1PLE IDS 101 102 DATE 09/22/73 l 21 PAGE PO T600 AQUA 9/28/77 I

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i PORTHERN IlOI ANA PUBLIC SERVICE COMPANY (49720) o BAILLT GEMERATIfG PLANT PHIT3PLC MTON DENSITY PA431R C? CELLS PER LITER STAT W W T NEARFIELD STATIONS LS TAXA 1-3 4-6 10  ; 3,g, "E'

3 6 10 0 UNIDENTIFIED ALGAE 25321.24 133%.21 232843.12 90500.19 71255.53 2.1 0 LtlIDENTIFIED ALGAE (LPIL) 25321.24 13136.21 232843.12 90500.19 71255.50 2.1 0 CYANOPHYTA 1389850A0 4549399.03 2170919.00 2703389.00 950145.75 61.6 0 OSCILLATORIACEAE O OSCILLATORIA 0 OSCILLATORIA (LPIL) 1141891.00 2387125.00 2170919.00 1899978.00 384147.75 43.3 0 LitC8YA 0 lyt:CBVA (LPIll 247959.00 1006745.00 0.0 418234.62 302836.12 9.5 0 OSCILLATORIACEAE (LPIL) 0.0 24029.21 0.0 8009.73 8009.73 0.2 0 HOSTf0.4CEAE O AH43Af tl%

0 AHADAEUA (LPIL) 0.0 13336.21 0.0 4445.40 4445.4b 0.1 0 AThAHirQTIEHON 0 FLOS-AGVE 0.0 1118163.00 0.0 372721.00 372721.00 8.5 f 0 CHLOROFHYTA 209227.81 292054.69 145617.06 215633.19 42394.05 4.9 u O VOLVOCALES N 0 CH L At1VD0tYt.AS 0 CHLAH100t:CW3 (17110 51145.16 0.0 13696.66 21613.94 15285.82 0.5 0 VOLVOCALES ( LPIL) 26311.98 15018.26 13696.66 18342.30 4003.06 0.4 0 TETRASTORALES 0 GLDEOCTSTIS 0 GLOEOCYSTIS (LPIL) 4445.40 0.0 0.0 1481.80 1481.80 0.0 0 CHLOR 0COCCALES 0 At*ISTRCDES*1US 0 Cot 4VOLUTUS 12247.39 0.0 0.0 4082.46 4082.46 0.1 0 FALCATUS 43320.62 29185.29 0.0 24168.64 12754.66 0.6 g 0 AIEISTROCESMUS (LPIL) 19223.37 0.0 0.0 6407.79 6407.79 0.1

(# 0 00CTSTIS

(( 0 COCYSTIS ( LPILI 0.0 10034.01 0.0 3344.67 3344.67 0.1 j 0 r11CR 4CTIllIUtt g 0 HICRACTIllIUM (LPILI 0.0 78094.94 0.0 26031.64 26031.64 0.6 0 DICTYOSF11tERIUit lg 0 0

DICTYOSrHAERIUM ( LPIL)

SCEtaEDES!1US 0.0 0.0 13696.66 4565.55 4565.55 0.1

. O ACUt11HATUS 36734.69 0.0 0.0 12244.89 12244.89 0.3 0 0 SCENEDEst1US ( LPIL) 5226.35 17228.95 0.0 7485.10 5100.18 0.2 l 0 0

CRUCIGEllIA CUADRATA 0.0 34121.48 0.0 11373.82 11373.82 0.3 k 0 CHLOROCOCCALES (LPIL) 10572.85 26191.84 70285.44 35683.37 17878.90 0.8 1 0 ULO1 RICH 4LES S 0 ULOTHRIX

$ 0 ULOlHRIX (LPIL) 0.0 0.0 34241.62 11413.87 11413.87 0.3 3

DATE 09/22/78 PAGE NO 23 T600 AQUA 9/28/77 C U Q

l t40RTHERN 1 HOI ANA FUCLIC SERVICE COMPANY (49720) o EAILI.Y GENERATIts PLANT PHYTCPLAt4KTCt4 DENSITT HurSER OF CELLS PER LITER NEARFIELD STATIONS (continued)

STATI0t4 REPORT Je 4-6 10 REL LS TAXA X S.E. ABX 3 6 10 0 ULOTRICHALES (LPIL) 3 42179.94 0.0 27393.31 27393.31 0.6 0 XAHillOFHY T A 30Y 6668.11 0.0 12532.59 9397.79 0.3 0 HETER 0TRICHALES j 0 TRICOHEt1A i 0 TRIBOt4EttA (LPILI 30929.66 6668.11 0.0 12532.59 9397.79 0.3 0 CHR YSOP11YT A 371521.25 217671.37 159674.12 182955.56 17691.60 4.2 0 CHR YS0*10HAD A LE3 0 DIN 00RY0'4 0 DIVERGEt&S 15739.14 45826.91 41089.91 34552.00 9551.11 0.8 0 SOCIALE 11643.98 19419.85 0.0 10354.61 5642.97 0.2 0 DINOCRYOH (LFILI 9642.18 4505.48 0.0 4715.89 2785.45 0.1 0 KErtlYRIOtt 0 KEFHIRict ILPIL) 24629.95 36163.96 15498.85 25430.92 5978.93 0.6 0 CHRYSOCHR0t0 LINA

>* O PARVA 0.0 0.0 41089.97 13696.66 13696.66 0.3 b

W 0 CHRYS0;;OHADALES (LPIL) 104639.69 101864.37 61995.37 89499.81 13775.5* 2.0 0 ttCitOSIG A LE S 0 f t0!!OSIG A 0 t1CHOSIGA (LPIL) 5226.35 0.0 0.0 1742.12 1742.12 0.0 0 CHRYSOFHYTA ELPILI 0.0 8590.80 0.0 2963.60 2963.60 0.1 0 eaCILLARIOPHYTA-CENTRIC 155600.25 16732.15 30364.95 67572.44 44190.00 1.5 0 EUPODISCALES 0 #1ELOSIRA 0 ITALICA 2222.70 0.0 0.0 740.90 740.90 0.0 0 ISLAt4 DICA 27551.00 0.0 0.0 9183.66 9133.66 0.2 0 EUF0DISCALES ( LPIL) 112454.25 14479.41 16435.98 47789.88 32337.12 1.1 g 0 RHIZOSOLEHIALES g

0 RHIZOSOLEt4IA O ERIENSIS 13372.25 2252.74 13948.96 9857.98 3806.27 0.2 0 BACILLARICFHYTA-pet:NATE 820364.06 1834981.00 325007.06 993457.37 444398.87 22.6 O O FRAGILARIALES O O ASTERIONELLA g 0 FORMOSA 15759.62 2675.73 0.0 6145.12 4868.91 0.1 0 DIATCilA I O TftoE 32661.29 25465.84 8217.99 22115.04 7252.34 0.5 0 0 FRAGILARIA 4 0 CROT0liENSIS 24509.79 163398.56 12399.08 66769.12 48441.03 1.5 8 0 FRAGILARIA ( LPIL) 119501.00 9S1772.81 0.0 367091.25 309270.69 8.4

& 0 SYttED2A I O Ult!A 0.0 1706.07 0.0 568.69 568.69 0.0

{ 0 SYt!ECRA ( LPIL) 156835.50 30561.64 52479.82 79958.94 38955.53 1.8 g 0 TAGELLARIA 3 .

DATE 09/22/78 PAGE HO 24 T6004QUA 9/28/77

NcRTHERN ItotANA RJBLIC SERVICE CCftPANY (49720)

BAILLY GEtt.RATItG PLArn PHYTOPL/.gTCH CENSITY Hut *BER OF CELLS FER LITER STATICH EEPORT NEARFIELD STATIONS (continued) 1-3 4-6 10 -

ret LS TAXA X S.E. ABX 3 6 10 0 FEttESTRATA 42176.83 19733.54 0.0 20636.79 12183.77 0.5 0 FLOCCULOSA 160509.12 429208.94 137867.62 242528.56 93568.69 5.5 0 TABELLARI A (LPIL) 84183.56 43815.14 0.0 42666.23 24308.49 1.0 0 FRAGILARIALES (LPIL) 5226.35 66843.37 0.0 24023.24 21463.16 0.5 0 HAVICULALES 0 HAVICULALES ELPILI 18367.34 11134.74 0.0 9834.03 5341.93 0.2 0 BACILLARIALES 0 HITZCHIA 0 ACICULARIS 50701.60 19727.98 53416.94 , 41282.17 10805.56 0.9 0 HITZCHIA ELPIL) 54506.62 26462.15 46929.05 42632.61 8375.91 1.0 0 SURIRELLALES 0 SURIRELLA 0 SURIRELLA (LPIll 9183.67 0.0 0.0 3061.22 3061.22 0.1 y 0 BACILLARIOPHTTA-PEtalATE ILPIL) 46262.31 12475.77 13696.66 24144.91 11064.31 0.6 e O PIRRMO."H YTA-DINOPHYC E AE 6202.77 600.73 0.0 2267.83 1975.10 g 0 Gittr100!HI A LES 0.1 0 GYtt!ODINIL".1 0 GyFNODIt4IUt1 i LPIL I 4445.40 0.0 0.0 1481.80 1481.80 0.0 0 PERIDIMIALES 0 PERIDINItti 0 It. Tot;SPICLRJ1 0.0 600.73 0.0 200.24 200.24 0.0 0 PERIDINIUM (LPIL) 1757.37 0.0 0.0 585.79 585.79 0.0 0 CRYPTCPHYTA 140308.12 105770.94 113177.62 119752.19 10497.99 2.7 0 CRYPTONCt.T!D A LE S 0 CR V PTot10t14S 0 NARS$ct411 4445.40 4505.48 0.0 29S3.63 1491.91 0.1

1. 0 CRYPTCtt0ttAS tLPIL) 38458.09 6007.30 0 0.0 14821.80 11944.70 0.3 0 RH000M3t4AS O itIIAJTA 19343.51 81879.50 99420.94 66901.31 0 LENS 24315.71 1.5 0.0 0.0 13696.66 4565.55 4565.55 0.1 0 CHRoot1CttAS 0 CtsCCt10HAS iLPIL) 50510.19 13378.68 l 0 CRIPTOt:Of4ADALES iLPILI 27551.00 0.0 0.0 0.0 21296.29 9183.66 15108.89 9183.66 0.5 0.2 a

g TOTAL 2949341.00 7037211.00 3177615.00 4388055.00 1326215.00 100.0 DIVERSITY IH PRINE) 2.87 2.30 2.24 2.47 0.20 DIVERSITY IJ FRit1El 0.72 0.61 0.64 0.66 0.04 g hut 1SER OF TAXA 44 42 22 58 OATE 09/22/78 8

PAGE HD 25 I T600 AQUA 9/28/77 3

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4 NORTHERH INDIANA PUBLIC SERVICE Cot 1PANY (49720)

O BAILL) GEt4ERATIIG PLANT PHYTOPLAfEKT0t3 DENSITY Nut 1SER OF CELLS PER LITER l REPLICATE REPORT PC TC CC LOC 5 56 56 1 1 00 DURATION TOW sat 1P VOL WIto CUREtiT TEr1P SID DATE tit 1E D/H UNITS C SD W3 SP D UNITS C SECH W T SC DI CL SP DI AIR WAT BT TURBD C0to 00 PH SALN P 171 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 172 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 REL LS TAXA X S.E. AB%

1 2 0 CYAHOPHYTA 144175.3% 0.0 72087.62 72087.62 3.7 0 CHROOCOCCACEAE O C0:101105PH AERI A .

O Got1PH0$PHAERIA (LPIL) 144175.31 0.0 72087.62 72087.62 3.7 l 0 CHLOROPHYTA 1416521.00 1643598.00 1530059.00 113538.50 79.0 0 VOLVOCALES 0 VOLVOCALES (LPIL) 36043.83 0.0 18021.91 18021.91 0.9 0 TETRASPCRALES I 0 GLCEOCISTIS y 0 CLCEOCYSTIS (LPILI 216262.94 0.0 108131.44 108131.44 5.6 0 CHLCROCCCCALES 0 SPHALRCCYSTIS 0 SFHAERCCTSTIS (LPIL) 288350.62 1314879.00 801614.81 513264.19 41.4 0 AIMISTROCES?tOS 0 CCitiOLUTUS 36043.83 0.0 18021.91 18021.91 0.9 0 CLOSTERIOPSIS 0 CLC31ERIOPSIS T h*IL) 10813.15 0.0 5406.57 5406.57 0.3 0 KIR C.4HERIELLA 0 LUHARIS 36043.83 0.0 18021.91 18021.91 0.9 0 OCC1STIS S 0 OCCTSTIS (LPIL) 72087.62 0.0 36043.81 36043.81 1.9 0 0 SCENECISitus O CUCJRICAUDA 360438.25 0.0 180219.12 180219.12 9.3 0 SCENEDESTUS (LPILI 36043.83 190311.44 113177.62 77133.75 5.8 g 0 CitLOROCOCCALES ( LPIL) 324394.31 138405.25 231401.25 92993.00 12.0 0 BACILLARIorHYTA-CENTRIC 36043.83 0.0 18021.91 18021.91 0.9 l

g 0

0 EUFCOISCALES EUPODICCA!ES ( LPIL) 36043.83 0.0 18021.91 18021.91 0.9 0 B ACIL LARIorilYTA-pef 24 ATE 97318.31 0.0 48659.16 48659.16 2.5 I O ACitttAl4TH A LES l 0 0

ACHHANTHES

/C!IH*fl1HES (LPIL) 61274.50 0.0 30637.25 30637.25 1.6 E O EACILLARIALES k 0 HITZCHIA S 0 HITZCHI A (LPIL) 36043.83 0.0 18021.91 18021.91 0.9 0 0 PYRPHOPHYTA-DINOPHYCEAE 3604.38 0.0 1802.19 1802.19 0.1 2

DATE 09/22/7S PAGE NO 43 T600 AQUA 9/28/77

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ICRTHERt4 ItCIAttA PUBLIC SERVICE COMPANY (497201 O

BAILLY CENERATItG PLAPIT FHYTOPLAlaKTOtt DENSITY IAX13ER OF CELLS PER LITER REPLICATE REPORT PC TC GC LOC ,

5 56 56 1 1 00 DURATICH TCH SAMP VOL WIPC CURENT TEMP SID DATE TIME D/N UIITS C SD WD SP O UNITS C SECH W T SC 01 CL SP DI AIR MAT BT TURBD Coto 00 PH SAlti P 181 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 i 182 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 1

- REL LS TAXA X S.E. ABX l 1 2 0 LRIIDEtITIFIED ALGAC 0.0 90830.37 45415.19 45415.19 1.8 0 UtlIDEtiTIFIED ALGAE ELPIll 0.0 90830.37 45415.19 454!3.17 1.8 0 CYAliOPH T T A 804498.37 0.0 402249.19 40224?.19 16.2 0 CHROOCOCCACEAE O GOMPHOSPHAERIA 0 GO:17HOSPHAERIA (LPILI 804493.37 0.0 402249.19 402249.19 16.2 i 0 CHLC20PitTTA 1632460.00 1665223.00 1648841.00 16M1.50 66.6 0 VOLVOCALES I O VOLVOCALES (LPIL) 33520.77 0.0 16760.38 16760.38 0.7 W 0 TETRASPORALES O GLCEOCISTIS 0 GLOCOCVSTIS (LPIL) 167603.81 0.0 83801.87 83801.87 3.4 0 CHLORO CCCALES O SPHAEROCYSTIS 0 SFH AER001STIS (LPIL) 268166.12 938580.81 603375.44 335207.31 24.4 0 CLOSTERIOPSIS 0 CLOSTERIOPSIS (LPILI 13408.30 0.0 6704.15 6704.15 0.3 0 KIRSCH?iERIELLA 0 Lut41RIS 351968.00 0.0 175984.00 175984.00 7.1 0 00CTSTIS 0 OCC1STIS (LPILI 33520.77 0.0 16760.38 16760.38 0.7 8 0 SCE!4EDES!!US 0 QUACRICAUDA 636894.50 121107.19 379000.81 %57893.62 15.3 0 SPIT:0 SUS 26816.61 121107.19 73961.87 47145.29 3.0 0 SCEtiEDES:'L'S i LPIL ) 0.0 121107.19 60553.59 6k$53.59 2.4 O O CHLCPOCOCCALES (LPIL) 0.0 363321.56 181660.75 181660.75 7.3 0 Z)CiaEf tATALES 0 HYALOTHECA O HYALO1HECA ELPIL) 100562.25 0.0 50281.12 50281.12 2.0 0 0 CliRySCrHYTA 0.0 30276.80 15138.40 15138.40 0.(

l 0 0

CHRYSOM0tIADALES CH2fSC';Ct!ADALES (LPIL) 0.0 30276.80 15133.40 15138.40 0.6

& 0 DACILLA!?IorHYTA-CENTRIC 33520.77 30276.20 31818.79 1621.98 1.3

{ 0 EUPC31 SCALES g 0 EUTCDISCALES ELPIL) 33520.77 30276.80 31898.79 149275.37 1621.93 5136.19 1.3 g 0 BACILLARIDFHYTA-Peta! ATE 144139.19 154411.56 6.0 3

DATE 09/22/78 PAGE tio 45 T600 AQUA 9/28/77

HORTHERtl INDI AllA PUBLIC SERVICE C0ttPANY (497201 BAILLY GENERATING PLANT PHTTCPLAtKT0t1 DENSITY DA#tBER CF CELLS PER LITER REPLICATE EEPORT REL LS TAXA X S.E. A8%

1 2  ;:

0 FRAGILARIALES O ASTERIONELLA 0 F C2t*,05 A 33520.77 0.0 16760.38 16760.38 0.7 0 FRAGILARIA 0 FRAGIL ARI A ( LPIL) 50281.15 54498.25 52389.70 2108.55 2.1 0 ELAIOTIALES 0 Eul#0TI A 0 Eutt0TI A ( LPIL) 26816.61 0.0 13408.30 13408.30 0.5 0 AClitlAllTHALES 0 ACHilANTHES 0 ACit!!ANTHES (LPIL) 0.0 30276.80 15138.40 15138.40 0.6 0 SL'RIR E L LA LE S 0 SL'RIR E L L A y 0 SURIRELL4 ( LPIL) 0.0 30276.80 15138.40 15138.40 0.6 e 0 B ACILLARICPHYTA-pet 24 ATE (LPIL) 33520.77 39359.84 36440.30 2919.54 1.5 y 0 CRYPT 0FMYTA 368728.31 0.0 184364.12 184364.12 7.4 0 CR YPTCM0 tlc 0 ALE S 0 RHCCCMCtlAS 0 ftItAJTA 368728.31 0.0 184364.12 184364.12 7.4 TOTAL 2983345.C0 1971015.00 2477180.00 506165.00 100.0 DIVERSITY IH PRIME) 3.05 2.53 2.79 0.26 l DIVERSITY (J PRItEl 0.76 0.71 0.73 0.03 1

tiU:CER OF TAXA 16 12 22 ABOVE CCMNTED LIS1HG SAMPLE IDS l 181 182 DATE 09/22/78 gy PAGE NO 46 S T600 AQUA 9/28/77 9

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HORTHERN ItDIENA PUBLIC SERVICE CCt1PANY (49720) o BAILLY GENERATING PLANT FHYTCPLAt:KTOH DENSITY IRA 13ER OF CELLS PER LITER STATION REPORT POND B REL LS TAXA X S.E. AB%

17 18 0 UNIDENTIFIED ALGAE 0.0 45415.19 22707.59 22707.59 1.0 0 Lt! IDENTIFIED ALGAE (LPIL) 0.0 45415.19 22707.59 22707.59 1.0 0 CYAt:0PitYTA 72087.62 402249 19 237168.37 165080.75 10.7 0 CliROOC0CC ACE A E O GO;!PHCOPitAERIA 0 GO!!PtiOSPH AERI A ( LPIL) 72087.62 402249.19 237168.37 165080.75 10.7 0 CH LC'?OFHYT A 1530059.00 1643841.00 1589450.00 '

59391.00 72.0 O VOLYCCALES 0 VOLYCCALES (LPIL) 18021.91 16760.38 17391.15 630.77 0.8 0 TETRASP00 ALES 0 GLCEDCYSTIS 0 GLDEOCYSTIS (LPIL) 108131.44 83801.87 95966.62 12164.78 4.3 0 CHt000CCCCALES 0 SrilAECCCYSTIS 7

w 0 SPHAERCCYSTIS ( LPIL) 801614.81 603373.44 702494.12 99120.69 31.8 0 A!:KISTRCDESituS

• CC;NOLUTUS O 18021.91 0.0 9010.96 9010.96 0.4 0 CtCOTERIOPSIS 0 CLOSTERICFSIS ( LPIL) 5406.57 6704.15 6055.36 648.79 0.3 0 KIRSCHt!ERIELLA 0 LLt!ARIS 18021.91 175934.00 97002.94 78981.00 4.4 0 C001STIS 0 CCCYSTIS ( LPIL) 36043.81 16760.38 26402.10 9641.71 1.2 0 SCEH10ESt1US 0 QU1021CAU3A 180219.12 379000.81 279609.94 99390.81 12.7 0 SPII:0 SUS 0.0 73961.87 36930.94 36930.94 1.7 g 0 SCEtt! DES!:US (LPIL) 113177.62 60553.59 86855.56 26312.02 3.9 9 0 CHLC202CCCALES (LPIL) 231401.25 181660.75 206531.00 24870.25 9.4 0 ZYCt;E!!ATALES 0 HTALO1HECA g 0 HTALOTHECA (LPIL) 0.0 50281.12 25140.56 25140.56 1.1 0 CHRYSO('11YTA 0.0 15138.40 7569.20 7569.20 ' O.3 0 CH2 YS0!".CM AD ALES 0 CHRICOMOHADALES (LPIL) 0.0 15138.40 7569.20 7569.20 0.3 0 DACILLADIOPHYTA-CENTRIC 18021.91 31898.79 24960.35 6938.43 1.1 k 0 EUPODISCALES l 0 EUPCDISCALES (LPIL) 0 BACIL L ADIOPH V TA-pet 04 ATE 18021.91 48659.16 31893.79 149275.37 24960.35

'. 66 7. 25 6933.43 50303.11 1.1 4.5 k 0 FRAGILARIALES 1 0 AST ERIO:!E LLA S 0 FO*uCS A 0.0 16760.38 83S0.19 8330.19 0.4 0 0 rRAGILAPIA 3

DATE 09/22/78 PAGE Ho 47 T600 AQUA 9/28/77

HoRTHERH ItOIANA PUBLIC 5ERVICE COttPANY (49720)

BAILLY GENERATING PLAfiT PHYTOPLAliKTCH DEt4SITT tAREER OF CELLS PER LITER STATION REPCRT POND B (continued)

- REL X S.E. ASX LS TAXA 17 18 0.0 52389.70 26194.85 26194.85 1.2 0 FRAGILARIA (LPIL) 0 EUtiOTIALES 0 ELRt0TI A 0.0 13408.30 6704.15 6704.15 0.3  !

0 EUt40TIA (LPIL)

O ACHNANTHALES 0 ActftlAtJTHES 30637.25 15138.40 22837.82 7749.42 1.0 0 ACHHAt{THES ( LPIL) .

0 BACILLARIALES O tlITZCHIA l 18021.91 0.0 9010.96 9010.96 0.4 1

0 HITZCHIA (LPILI 0 SURIRELLALES 0 SURIRELLA 0.0 15138.40 7569.20 7569.20 0.3 0 EURIRELLA (LPIL) 18220.15 36440.30 18220.15 0.8

> 0 BACILLARIOPHfTA-PEte1 ATE ( LPIL) 0.0 901.10 j, O PIREHOPHYTA-DIt;OPHYCEAE 1802.19 0.0 901.10 0.0 0 0 PERIDI:4IALES 0 PERIDINIUM GATUtiftiSE 1802.19 0.0 901.10 901.10 0.0 0

0 CRYPTOFHYTA 265282.50 184364.12 224823.31 40459.19 10.2 0 CRIPTCH3?!CDALES 0 CRYPTCM0t#AS ttARSSottII 17301.04 0.0 8650.52 8650.52 0.4 0

0 Rif0 dot!CNAS 0 HItUTA 247981.44 184364.12 216172.75 31808.66 9.8 TOTAL 1935908.00 2477180.00 2206544.00 270636.00 100.0 g 2.3S 2.79 2.58 0.21 g DIVERSITY (H PRIME )

DIVERSITf (J PRINE) 0.72 0.73 0.73 0.01 HLISER OF TAXA 17 22 26 ACOVE COMPUTED USING SAMPLE IDS 171 172 181 182 4 DATE 09/22/78 PAC-E HO 48 T600 AQUA 9/28/77 I

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HORTHEPH ItOI AHA PUBLIC SERVICE COMPAHf (49720) o BAILLY GE?ERATING PLANT PHTTOPLAHKTOH DENSITY tEEEER OF CELLS PER LITER REPLICATE REFORT PC TC GC LOC 5 56 56 1 2 00 DURATION TOW SAMP VOL HINO CURENT TEMP SID DATE TIME D/H L' NITS C SD WD SP D UttITS C SECH H T SC DI CL SP DI AIR WAT BT TURBD C0t3D CO FH SAlti P 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 191 6/23/78 0.0 0.0 0 192 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0

- REL TAXA X S.E. AB%

LS 1 2 0 CVANOPHYTA 0.0 210884.37 105442.19 105442.19 10.7 0 CSCILLATORIACEAE 0 05CILLATCRIA 0 OSCILLATORIA ( LPIL) 0.0 210884.37 105442.19 105442.19 10.7 0 CHLO90PHYTA 205449.81 210884.31 203167.06 2717.25 21.2 0 VOLVOCALES 0 CONIU:1 0 G3NIUM ( LPILI 32439.45 0.0 16219.72 16219.72 1.7 3,

1 0 PAD 10 FILUM 0 RADIGFILUM ILPIL) 173010.37 0.0 86505.19 86505.19 8.8

[$

0 CHLCROCOCCALES 0 C10STERIOPSIS 0 CLOSTERICFSIS ILPIL) 0.0 52721.09 26360.55 26360.55 2.7 0 Z10!:E!!ATALES 0 C00ft1RIUM 0 COSitARIUtt (LPILI 0.0 105442.19 52721.09 52721.09 5.4 0 STAURASTRUM 0 PAR /DOXUM 0.0 52721.09 26360.55 26360.55 2.7 0 BACILLt.RIOP:1YTA-CENTRIC 0.0 42176.87 21088.44 21083.44 2.1 0 EUFCDIOCALES

1. 0 EUFDDISCALES ( LPIL) 0.0 42176.87 21088.44 21088.44 2.1

!! O BACILLARICrHYTA-PEttt! ATE 21626.30 959523.75 490575.00 468948.69 50.0 0 0 FRAGILARIALES

$ 0 ASTERICliELLA g 0 FCRHOSA 0.u 221428.50 110714.25 110714.25 11.3 g 0 FRAGILARIA g 0 FRAGILAPIA ( LPIL) 0.0 685374.19 342687.06 342687.06 34.9 g 0 ACHNAlliHALES

-- 0 COCCCt4EIS 0 COCCONCIS (LPIL) 0.0 52721.09 26360.55 26360.55 2.7 g 0 flAVICULALES g 0 i:AVICULA

-- 0 IIAVICULA ELPIL) 21626.30 0.0 10813.15 10813.15 1.1

f. O P1RPI:0PHYTA-DINOPHfCEAE 0.0 79081.62 39540.81 39540.81 4.0

!L 0 PEPIDIll! ALES O O FERIDINIU:1 2

DATE 09/22/78 PAGE No 51 T600 AQUA 9/28/77

NORTHEptl INDIANA PUBLIC SERVICE COMPANY (49720)

BAILLY GEt4ERATItG PLANT PHYTCPLAtETOtt DENSITY j nut 1E,ER OF CELLS PER LITER 1

REPLICATE REPCRT REL LS TAXA X S.E. ABX 1 2 0 PERIDINIUM (LPILI 0.0 79081.62 39540.81 39543.81 4.0 0 CRYPTCPHTTA 21626.30 210834.37 116255.31 94629.00 11.8 0 CRTPTO CtCOALES 0 CR YPT0tCilAS 0 CRYPTor10t1AS (LPIL) 21626.30 52721.09 37173.70 15547.39 3.8 0 EHODO Ot4AS 0 MItAJTA 0.0 52721.09 26360.55 26360.55 2.7 0 CHRCCMONAS 0 CllROCitot4AS ( LPIL) 0.0 105442.19 . 52721.09 52721.09 5.4 TOTAL 248702.31 1713434.00 981068.12 732365.81 100.0 DIVERSITY IH PRIME) 1.36 2.89 2,12 0.76 DIVERSITY (J PRIMEl 0.68 0.81 0.74 0.06 y tAJMSER CF TAXA 4 12 15 N ABOVE COMPUTED USItG SAMPLE IDS 191 192 DATE 09/22/78 PAGE NO 52 T600 AQUA 9/28/77 e

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NORTHERN ItOIANA PUBLIC SERVICE COMPANY (49720)

O BAILLY GENERATIllG PLANT PHYTOPLAt1KTote DENSITY t 4EER OF CELLS PER LITER REPLICATE REPORT PC TC CC LOC 5 56 56 1 2 00 DURATI0t4 TOW SAMP VOL WItO CURENT TEMP TIME D/H L23ITS C WD SP D UtlITS C SECH H T SC DI CL SP DI AIR WAT BT TURDD Cota DO PH SALN P SID DATE SO 0.0 0 0.0 0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 201 6/23/78 0 0 0.0 202 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0

- REL X 5.E. ABX i LS TAXA l 1 2 0 CYAHOPHYTA 0.0 1397058.00 698529.00 698529.00 27.5 0 CH200COCCACEAE D GG:;FUO5PUAERIA 0 GCitPHOSFHAERIA (LPIL) 0.0 1004901.94 502450.94 502450.94 19.8

(

0 OSCILLATORIACEAE O 03CILLATORIA 0 00CILLAT031A (LPIL) 0.0 392156.87 196078.44 196078.44 7.7 CH.CROPHYTA 560661.56 2899504.00 1730082.00 1169421.00 68.0 p

s 0 VOLVOCALES

& O CHLAinCDrotlAS 0 CHLA!1tDOMOtlAS (LPIL) 0.0 4?019.61 24509.60 24509.80 1.0 0 00*11UM .

O GCilIUtl ( LPIL ) 472714.75 0.0 236357.37 236357.37 9.3 0 RADICFILUM 0 RADICFILUM (LPIL) 0.0 1862744.00 931372.00 931372.00 36.6 0 CHLCR0000CALES 0 00 CYSTIS 0 00CTSTIS (LPIL) 0.0 367646.94 183823.44 183823.44 7.2 0 SCEllEDESt105 0 SCENEDES!:US tLPILi 43973.48 0.0 21986.74 21986.74 0.9 0 PEDIASTRU'1 l g 0 TETRAS 0.0 98039.19 49019.59 49019.59 1.9 0 TETRAEURCH 0 CALDATUM 21986.74 0.0 10993.37 10993.37 0.4 HINItlUN 21986.74 0.0 10993.37 10993.37 0.4 g 0 0 COELASTRUM 0 itICR000?UN 0.0 392156.87 196078.44 196078.44 7.7 0 CHLOROOCCCALES (LPIL) 0.0 24509.80 12254.90 12254.90 0.5 0 2TGtEt1ATALES 0 COOtt RIUll l 0 0

COSilARIUtl ( LPIL)

STAURASTRUM 0.0 80882.31 40441.16 40441.16 1.6 E O JO:tN3CNII 0.0 24509.80 12254.90 12254.90 0.5 12254.90 12254.90 k

9 0 EUGLEt:OrilYTA 0 EU3LE!! ALES 0.0 24509.80 0.5

[

3 0 T R ACHELO!!Ot3AS DATE 09/22/78 PAGE Ho 53 T600 AQUA 9/28/77

i l

l HOR 1HERt4 INDIAt4A PUBLIC SERVICE COMPANY (49720) .

o BAILLY GENERATItG PLANT l

PHYTOPLAta(Tort DENSITY l HUMBER OF CELLS PER LITER REPLICATE REPORT

- REL LS TAXA X S.E. ABX 1 2 0 TRACHELOMONAS (LPIL) 0.0 24509.80 12254.90 12254.90 0.5 0 CHRfSOPHYTA 0.0 49019.61 24509.80 24509.80 1.0 0 CHRYSO:1CilADALES 0 CHR YSO**.Ot!AD ALES ( LPIL ) 0.0 49019.61 24509.80 24509.80 1.0 0 BACILLAR 10FHYTA-PEtalATE 21986.74 83333.31 52660.02 30673.29 2.1 0 FRAGILARIALES 0 FRAGILARIA 0 FRAGILARIA ( LPIL) 0.0 49019.61 24509.80 24509.80 1.0 0 SfHEDRA 0 SitlEDRA ( LPIL) 0.0 9803.92 4901.96 4901.96 0.2 0 BACILLARIALES 0 HAtiTZSCHIA 0 HANTZSCHIA (LPILI 0.0 24509.80 12254.90 12254.90 0.5

> 0 BACILLARIOPHYTA-pet?!! ATE ( LPIL) 21986.74 0.0 10993.37 10993.37 0.4 0 PIEFHOFHfTA-DitOPHYCEAE 0.0 49019.61 24509.80 24509.80 1.0

[ 0 PERIDIllIALES d

0 FERIDINIUtt 0 G %TU:#EttSE 0.0 24509.80 12254.90 12254.90 0.5 0 PERIDittIUtt (LPIL) 0.0 24509.80 12254.90 1L254.90 0.5 TOTAL 582648.25 4502437.00 2542542.00 1959894.00 100.0 DIVERSITY (H PRINE) 1.06 2.62 1.84 0.78 OIVERSITY (J PRINE ) 0.46 0.64 0.55 0.09 tLACER OF TAXA 5 17 22 ABCVE CCitPUTED USIllG sat 1PLE IDS l 201 202 DATE 09/22/78 h

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j

i HCRTHEEN IPCIANA PUBLIC SERVICE COMPAHf (49720) o BAILLY GEt:ERATItG PL ANT FHYTOPLANKT0t4 DENSITY HurtSER OF CELLS PER LITER STATICH REPORT POND C

- REL LS TAXA X S.E. ADX 19 20 0 CYA!;0PHYTA 105442.19 698529.00 401985.56 296543.37 22.4 0 CHRCOCOCCACEAE O CO:trHOSPHAERIA 0 GCt1FHOSFMAERIA (LPIL) 0.0 502450.94 251225.44 251225.44 I4.3 0 O!CILLAT02IA0EAE O OSCILLATCRIA 0 OSCILLATORIA ELPIL) 105442.19 196078.44 150760.31 45318.12 8.6 0 CHLCDOPHTTA 208167.06 1730082.00 969124.50 760957.44 55.0 0 VOLVOCALES 0 CH L At1100t:0tlAS 0 CHLaf1VD0ttallAS (LPIL 3 0.0 24509.80 12254.90 12254.90 0.7 0 G0:41Ut1 0 CClILil (LPIll 16219.72 236357.37 126288.50 110068.81 7.2 0 RtDIOFILLtl i 0 RADICFILLR1 ( LPIL) 86505.19 931372.00 508938.56 422433.37 28.9 e O CHLC?CCCCCALES

" 0 CLOSTERIOFSIS 0 CLOSTER10PSIS (LPILI 26360.55 0.0 13180.27 13180.27 0.7 0 00CVSTIS l

0 00 CYSTIS (LPIL) 0.0 183823.44 91911.69 91911.69 5.2 0 SCE!;EDESt1US 0 SCENEDES!!US (LPIL) 0.0 21986.74 10993.37 10993.37 0.6 0 FEDIASTRtst 0 TETkAS 0.0 49019.59 24509.80 24509.80 1.4 0 TETRAEDRCN I 0 C AL.'D ATutt 0.0 10993.37 5496.68 5496.68 0.3 0 t11 hit 2.K1 0.0 10993.37 5496.68 5496.68 0.3 8 0 CCELASTRUt1 0 ftICRCPORUt1 0.0 196078.44 98039.19 98039.19 5.6 0 CitLOR020CCALES (LPILI 0.0 12254.90 6127.45 6127.45 0.3 0 ZYCt:E!14 TALES

• O CCSt1ARILR1 0 COSitGIUrl i LPILI 52721.09 40441.16 46581.12 6139.97 2.6 1 0 0

STAtDASTRUt1 PARAD0xutt 26360.55 0.0 13180.27 11180.27 0.7 0 JOHttSONII 0.0 12254.90 6127.4S 4127.45 0.3 0 EUGLEt!OF' LYT A

. 0.0 12:54.90 6127.45 $127.45 0.3 0 0 EL'3LEHALES

& 0 1RACliEL0t:0:4AS 12254.90 6127.45 6127.45 0.3 4

0 TRACHELor:CitAS E LPIL) 0.0 O CitR TSOPilYTA 0.0 24509.80 12:54.90 12:54.90 0.7 0 CHRT50t:CitADALES 3 s DATE 09/22/78 PAGE NO 55 T600 AQUA 9/28/77

HORTHERN ItCIANA PUBLIC SERVICE CCitPANY (49720)

BAILLY GENERATItG PLANT PHYTOPLAta(TCil DENSITY PARCER OF CELLS PER LITER STATICH REFCRT POND C (continued) - REL X S.E. ABX LS TAXA 19 20 0.0 24509.80 12254.90 12254.90 0.7 0 CHRYSot10ttADALES (LPIL) 10544.22 10544.22 0.6 l 0 BACILLARICFHYTA-CEtiTRIC 21088.44 0.0 l

0 EurCDISCALES 10544.22 10544.22 0.6 I 0 EUFCDISCALES (LPIL) 210$8.44 0.0 2 490575.00 52660.02 271617.50 218957.44 15.4 O BACILLARIOPHYTA-PDa4 ATE  ;

0 FW4GILARIALES l e ASTERIC:lELLA 55357.12 55357.12 3.1 0 FORrIOSA 110714.25 0.0 0 FRAGILARIA 183598.37 159088.62 10.4 0 FRAGILARIA (LPIL) 342687.06 24509.80 0 SYHIDPA 2450.98 2450.98 0.1 0 SYt1EDRA (LPIL) 0.0 4901.96 0 ACHel*t4THALES 0 CCCCCt;EIS 13180.27 13180.27 0.7 y 0 CCCCOttEIS (LPIL) 26360.55 0.0

c. O HAVICULALES

• O ttAVICULA 10S13.15 0.0 5406.57 5406.57 0.3 0 IIAVICULA (LPIL) 0 EACILLARIALES 0 H At4T25 CHI A 0.0 1225'+ . 90 6127.45 6127.45 0.3 0 HANTZSC:lIA ( LPIL) 5496.68 0.0 10993.37 5496.68 0.3 l 0 BACILLARICPilVTA-PittlATE (LPILs 32025.31 7515.50 1.8 0 PrREHOFHTTA-DII:OPHYCEAE 39540.81 24509.80 l

O PERIDIllI ALLS 0 PERIDI!!!U:1 6127.45 CATUNENSE 0.0 12254.90 6127.45 0.3 0 1.5 39540.81 12254.90 25$97.86 13642.95 g 0 FERISillIU:1 i LPIL) 58127.66 53127.66 3.3 0 0 CRYPTCF11YTA 116:55.31 0.0 0 CR V PT0t*Ott30 ALES 0 CRYPT 0i10tlAS 37173.70 0.0 18586.85 18586.85 1.1 0 CRYPT 0iOt!AS ILPIL) 0 RHCDO:04AS tIIt:UTA 26360.55 0.0 13180.27 13180.27 0.7 0

i 0 0

CI: ROC't3:lAS CitRCOiCIAS (LPIL) 52721.09 0.0 26360.55 26360.55 1.5

1. TOTAL 981068.12 2542542.00 1761805.00 780736.94 100.0 0 2.12 1.84 1.93 0.14 OIVERSITY (H PRIMER

& DIVERSITY (J PRIttEl 0.74 0.55 0.65 0.10 15 22 31

{ tar 3ER OF TAXA

)3 AeovE Connmo uSitG SinPtE IoS 191 192 201 202 DATE 09/22/78 PAGE tm 56

')

L__._.

NORTHERN ItDIANA PUBLIC dERVICE COMPAHf 1497203 o

BAILLY GENERATIN3 PLANT PHYTCPLAJETO 4 DENSITT Nun 8ER CF CELLS PER LITER REPLICATE REPORT COWLES BOC (21)

PC TC CC LOC 556561300 DURATIOtt TOW SAMP VOL Wito CURENT T Et*P SID DATE TIME D/N UNITS C SD WD SP D Ut4ITS C SECH W T SC DI CL SP DI AIR HAT BT TURSD COta DO PH SALN P 211 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 212 6/23/78 0 0 0.0 0 0.0 0.0 0.0 0 2.0 4 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0

- REL LS TAXA X S.E. ABX 1 2 0 UNIDENTIFIED ALGAE 6721088.00 0.0 3360544.00 3360544.00 13.3 0 UNIDENTIFIED ALGAE ILPILI 672108S.00 0.0 3360544.00 3360544.00 13.3 0 CYAICPH)TA 25360944,0 306372.56 12843655.0 12537285.0 50.7 0 CHROOCCCCACEAE o t11CROCYSTIS 0 NICROC)STIS ILPILI 12027210.0 306372.56 6166791.00 5860418.00 24.3 0 OSCILLATORIACEAE y 0 LYNCBYA

O LYNGSYA ELPIL) 13353740.0 0.0 6676870.00 6676870.00 26.3

• 0 CHLO20PHYTA 12911564.0 490196.12 6700820.00 6210683.00 26.4 O CHLC20CCCCALES 0 SFHAEROCYSTIS 0 SP:t AERCCYSTIS E LPIL) 0.0 490196.12 245098.06 245098.06 1.0 l 0 AIEISTRODESt'US 0 FALCATUS 176870.75 0.0 88435.37 88435.37 0.3 0 CLOSTERIOPSIS 0 CLOSTERIOPSIS (LPIL) 176870.75 0.0 88435.37 88435.37 0.3 0 TETR/ZDR0!4 0 t1UTICUN 176870.75 0.0 88435.37 88435.37 0.3 0 CRUCIGENIA

RECTANJULARIS 2829932.00 0.0 1414966.00 '1414966.00 5.6 8 0 CHLCEOCOCCALES (LPILI 9551020.00 0.0 4775510.00 4775510.00 18.8 0 EUGLEt:GFHYTA 0.0 61274.52 30637.26 30637.26 0.1 0 EUGLEHALES g 0 EUGLENA 0 EUGLENA (LPIll 0.0 61274.52 30637.26 30637.26 0.1 0 CHRYSCPHYTA 353741.50 61274.52 207508.00 146233.44 0.8 0 CHR) S0;t0tI AD ALES 0 CH9YSCCCCCUS 0 CHRISOCCCCUS (LPIL) 0.0 61274.52 30637.26 30637.26 0.1 l 0 CH'?YSO."0!LtD ALES ( LPILI 0 B ACILLARIOPIYTA-CDITRIC 353741.50 854353.75 0.0 122549.00 17eS70.75-503451.37 176870.75 360902.37 0.7 2.0 0 0 EUPODIOCALES 0 t'ELO3 IRA

& 0 i:ELOSIR A (LPILI 0.0 61274.52 30637.26 30637.26 0.1 0 0 EUPODISCALES (LPILI 884353.75 61274.52 472814.12 411539.56 1.9 3

OATE 09/22/78 PAGE NO 61 T600 AQUA 9/28/77

s .

PORTHERN ItOI ANA PUBLIC SERVICE CortPANY (49720) ,

BAILLY GENERATING PLAT 4T

~

PHYTCPLAliKTCH DEt4SITY Hut 1GER CT CELLS PER LITER REPLICATE REPO9T

- REL X S.E. AB%

LS TAXA 1 2 0 BACILLARIOPHYTA-pet #{ ATE 1644897.00 1145833.00 1395365.00 249532.00 5.5 0 FRAGILARIALES 0 01 AT0ttA 0.0 18382.36 9191.18 9191.18 0.0 0 TEliUE 0 FRAGILARIA 30637.26 FRAGILARIA (LPIL) 0.0 61274.52 30637.26 0.1 0

176870.75 0.0 88435.37 88435.37 0.3 0 TRAGILARIALES (LPILI 0 EUt0TIALES 0 Eut.0TIA 61274.50 0.0 122549.uo 61274.50 0.2 0 LUNOTIA ILPIL) 0 HAVICULALES 0 HAVICULA HAVICULA (LPIL) 406802.69 367647.00 387224.81 19577.84 1.5 0

0 PItc0LARIA 15318.63 0.1 0 PItscLARIA (LPIll 0.0 30637.26 15318.63 i

> 0 GottPUCtlEttr C GCt1PHOMEttA (LPIL1 0.0 61274.52 30637.26 30637.26 0.1 0

353741.50 61274.52 207508.00 146233.44 0.8 0 HAVICULALES (LPIL) 0 DACILLARIALES 0 HITZCHIA

1. 41TZCitIA (LPIll 353741.50 122549.00 238145.25 115596.25 0.9 0

353741.50 300245.06 326993.25 26748.22 1.3 3 BACILLARIC?HYTA-PEtalATE (LPIL) 126025.37 1.2 0 CR TPToritVTA 176870.75 428921.56 302896.12 0 CR YPTOttotiOD ALES 0 CRYPT 0tto:lAS 0 CRYPT 0tt0NAS (LPILI 0.0 428921.56 214460.75 214460.75 0.8 0 CR)PTCtDNADALES (LPIL) 176870.75 0.0 88435.37 88435.37 0.3 g

l TOTAL 48073296.0 2616416.00 25344848.0 22728432.0 100.0 DIVERSITY (H PRIME) 2.64 3.43 3.03 0.40 DIVERSITY (J PRIME) 0.66 0.86 0.76 0.10 Nutt3ER OF TAXA 16 16 26 ABOVE CCttPUTED USIttG sat 1PLE IDS 211 21E g DATE 09/22/78 PAGL HO 62 b T600 AQUA 9/28/77 i

e E

3 l

q_ ,)

e78265eemenese*eseen****eeeeeeeeeeeme=** **emene o e e e eeemeneseeeeeseeeenenoemennme**e78265e e e e e JC2NA:1E PAXEER ORIGIti tiAME e e e e e o e e e JCSilea1E tA)M3ER ORIGIta tiArtE

• o GPH)B4 1751 /,/ e e LCCAL ECO DLLTCH e . **

e **eene e GPHYB6 e e 1751 LOCAL ECO DALTON

• _/_ /_ e e eneeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeen enemoneen o e / // / .

eeeeeeeeeeeeeeeeeeeeeeenseeseseneeeeeene o e e / / / / s e FROCHAME STEPt1AME ODNAME FRTY e e e a //// =*

• FORT 1S e = een CTAPE CLASS LINES

• a / /_/ / e GO FT06F001 001 * ***

e

• emene / / e TI-12461 7001 e = ee A 00003647

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e e e emetomeneotehneeketteeeeeeenode#4eemette en e enesee (33 M APPENDIX B PHYTOPLANKTON BIOVOLUME REPLICATE REPORTS JUNE 1978 e

0 F

3 0

e a

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3 ,

SREPORT

• It#4IT so.

LASEL18'HORTHERH ItOI ANA PUBLIC SERVICE cot 1 PANT (49720 P .

LABEL 28'tAILLY GENERATItG PLAPIT*.

LAB E L 3 a ' PH TTCPL Ata(TON SICVOLUrtE ' ,

L18EL48't1ICROLITERS PER LITER *.

RLAB1** REPLICATE REPORT' .

l RLA82** STATION REPCRT*.

PLA832' STATION REPCRT*.

l RLAB4 s

• SITE SumARY ' .

SPEC 1 = 4. 999.5.

• R All' , ' Y E S * . 3.4 SPEC 288.999.4.*RAN' 'YES'.3.7.

SPEC 3 7.999.4.'RAN' '1ES',3.7, a SPEC 486. 999. 6. 'R AN' , ' YES' . 3. 3.

6 N

REPTOT:60.

LSTAT=1.

ftAXTXA = 50 3.

SID = STATION NUMBER GEARTB=56 1.0 11 = STATION 1 REPLICATE 1 ISIzt=22ao 12 = STATION 1 REPLICATE 2 LISTzS.

Str1LE V:1. -l . -l . 0. 0 JCTz30.

STLt4IT:20.

NLIST80.

STFILEal.

S g 11000LE z ' G56 56 068 ' ,

I

= IBIO:1.

JCT 2=31.

AEte

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. ooM N o e4 o oO of 4 e4 s-. o o Q f M **

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= g c.4 e9 ** 6

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.e p o.

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- p IL o noo N

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oo

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n o5 s

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- .c s-W >= w = .J MN

=

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a. @

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• .*. a is sa

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a.

~ =<n_w r, p oao w a

s w

s. ws <. <

w <

> - c s4

<Bu~ 0; - a mg P. _ <

wa-op =

.e

.e w=o>o .

w o n c. ow a.

w g a- s -

oo neou8am < <-.w s-a=

o -w

= e .a a 8 o e-

. w < <aw-4 6-ae- e--< < < w es o o a

--u>.o<w 5 s u .e E <a.c g *a f .< -

vcI <,<d:a>.< -

= >.

w a 8o e

c. cc.

r t u ci S >. - : - c- D.:.o.8n u..ocd. - =

e.. < m e.g cy e=- 8.

a. . w s a g a.,. .a o a w ao wss <

-nM x >-.- sou <.

< =,

< .= b. c 5

• w=oo s

. u.s~ : .m -- o >- r s~ > 8o. ou- c. oc s --

• . = g a w a o < u ea 4  : = E w o o e - o d = = b- w u w w u e ua:oc-. as o o foo- .= oo =.eo .o..c. ~.-

easa:

w a 3 s ~ a E .Js**nc < - .a w oss w g u o o a se-s u u s 4 n =r--uao z o -..i-d .-

a M

< u ue an eo d.

->o

=

u i-.

• e

= -

at u

-s uuw a-a

4. u w

> c. s u a o N.

>O n = s = < <

.r- s a o e e4

u. no - 55v v x v o o m .=

c.

U J

Q.

o.

o e 9

.u e cocooooooooooooooooooooooooooooooooo o o c =

.u 2e s B-3 solence servloes division

toRTHERN ItOI ANA PUBLIC SERVICE cot 1PANY (49720 8 o B AILLY GEt;ERATItG PLANT PHYTOPL ANKTOte BIOVOLUt*!

NICROLITERS PER LITER REPLICATE REPC97

- REL X S.E. ABX LS TAXA 1 2 0 F CPt".OS A 0.01 0.03 0.02 0.01 0.8 0 DIATOHA 0 TENUE 0.09 0.02 0.C6 0.04 2.4 0 FPtGILARIA 0.25 0.13 0.13 5.2 0 FRAGILt.RIA (LPIL) 0.0 0 SYt! EOR A 0.02 0.40 0.21 0.19 8.6 0 SYNEOPA (LPILI 0 TABELLARIA FEt4ESTRATA 0.0 0.47 0.24 0.24 9.7 0

0 TABELLARI A ( LPIL) 0.99 0.36 0.68 0.31 27.8 0 t4AVICULALES 0 HAVICULALES ( LPIL i 0.04 0.0 0.02 0.02 0.9 0 BACILLARIALES to 0 HITZCHIA j, O HITZCHIA (LPIL) 0.01 0.05 0.03 0.02 1.2 0 SURIDELLALES 0 SURIRELLA 0 SU11PELLA (LPIL) 0.10 0.0 0.05 C.05 2.0 0 B ACILLADIOPHYTA-pet: HATE ( LPIL) 0.04 0.09 0.06 0.C3 2.6 0 P1RRHorHYTA-DINCPHYCEAE 0.0 0.23 0.11 0.11 4.7 0 PERIDIt3IALES 0 PERIDINIUt1 0 PERIDIllIUt1 ( LPIL) 0.0 0.23 0.11 0.11 4.7 0 CRYPTOPHYTA 0.16 0.10 0.13 0.03 5.4 0 CRfPTCMOHODALES g 0 CPYPTOMOtAS O O CRYPT 0tt3'AS ELPIL) 0.12 0.10 0.11 0.01 4.6 T 0 CHRCCM0taS CHrOct10tiAS ( LPIL) 0.01 0.0 0.C1 0.01 0.3 3 0 0 0 CRYPTCMCHAD ALES ( LPIL ) 0.02 0.0 0.01 0.C1 0.5 0

TOTAL 2.27 2.60 2.44 0.16 100.0 g 3.46 3.11 0.34 DIVERSITY IH PRIME) 2.77 DIVERSITY IJ PRIt1E l 0.63 0.83 0.73 0.10 0 tt#EER OF TAXA 21 18 27 0

8 ABOVE COMPUTED USItG S AP1PLE IDS g 11 12 DATE 09/22/78 I PAGE HD 2

{ T600 AQUA 9/28/77 2

).

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=

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>=

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MOO HMOO

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. sOO ww w . s. .4 a .4 w .4 w a. w a <

s = a -< <

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o.

o

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c. .s wo . a.

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<=o

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. O

.- oo ww a .- - o es

• n.> m, o wn wu uu 4 :<Cu w 3. wa < o<

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o

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e a

~

w w

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u. u. s < a .4 >. s v. .u.

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~

h e u , vi s o es o n o e e o < n o o - m < .i.;. p:

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< ==

e- r weuoc

<oo u w w a - o E h. n n = a =< - w-o.. . ~ r4 e..~=~w 2t.am- <s e-4 = g a w w O <NN ANN > ww u o o u J e e- o o ou ns .o oa on nerwawa we p o B a n n o > > o; a a -a o *.

.4 < o a

w .

= o < > > v4 a < om 44 oc=n

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=

u

>

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= b o.

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o.

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eg = M us yM @ efB OODOOOOOOOOOOOOOOOOOOO000OOOOOOOOOOO G & = E E =4 B-S solence servlees divlelon

HORTHERN IHOIAHA PUSLIC SERVICE COMPANT (49720) o BAILLY gel 4ERATING PLANT PHVTOPLAt.YTDti BIOVOLUME MICROLITERS PER LITER REPLICATE REPCRT

- REL LS TAXA X S.E. AB%

1 2 0 SYNE 0RA 0 SYNEDR A ( LPIL) 0.01 0.0 0.01 0.01 1.5 0 TABELLARIA 0 FLOCCULOSA 0.30 0.09 0.20 0.10 43.6 0 FRAGILARI ALES (LPIL) 0.0 0.08 0.04 0.04 8.4 0 BACILLARIALES 0 HITZCHIA 0 ACICULARIS 0.01 0.02 0.01 0.01 3.0 0 HITZCHIA (LPIL) 0.0 0.01 0.01 0.01 1.4 0 BACILLARIOP;tYTA-pdc 1 ATE E LPIL) 0.02 0.0 0.01 0.01 2.4 0 PYRRHOPHYTA-DItt0PHYCEAE 0.02 0.0 0.01 0.01 2.7 0 GYtitlODINI ALES 0 GYtt1001HIUt1 to 0 GYtpiODINIUti ( LPIL ) 0.02 0.0 0.01 0.01 2.7 4 0 CRYPTOPHYTA 0.14 0.00 0.07 0.07 15.9 0 CRY PT0t10t100 AL ES 0 CRYPTOHONAS 0 MARSS0t4II 0.02 0.0 0.01 0.01 2.9 0 CRYPTot10NAS ( LPIL) 0.12 0.0 0.06 0.06 13.2 0 RHC00MONAS 0 MItRJTA 0.00 0.00 0.00 0.00 0.8 TOTAL 0.65 0.24 0.45 0.20 100.0 DIVERSITY IH PRIME l 2.75 2.35 2.55 0.20 DIVERSITY (J PRIME ) 0.66 0.71 0.60 0.02 Hut 18ER OF TAXA 18 10 23 t ABOVE COMPUTED USING SAttPLE IDS

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!BAILLY GEidRATING PLANT Ptif TCPLAkKTCH BIGVOLlri MICACLITEES PER LITER GtPLICAtt REPORT REL LS TAXA X S.E. ALZ 1 2 0 FLOCCULOSA 0.10 1.92 1.01 0.91 63.1 0 FRAGILAHIALES (LPILI 0.01 0.0 0.01 0.01 0.4 l 0 BACILLAPIALES l

0 HIT 2 CHIA 0 ACICULARIS G.0 0.01 0.01 0.01 0.*

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W O!viESITY 4J FRItEl 0.63 0.46 C.55 0.c3 tu1aEri OF TAXA li 14 21 ABOVE Cor1PLITED USit4G SAMPLE IDS 41 42 DATE 09/22/78 PAGE t!O 11 T600 AQUA 9/28/77 5

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O BAILLY GENERATING PLANT PERIPttYTD4 DENSITY DENSITY IN HUMEER / SQUARE CENTIMETER REPLICATE REPORT PC TC GC LOC 566660020 DURATICH TOW SAMP VOL WIto CURENT TEMP SID DATE TIME 0/H UNITS C SO 10 SP O UNITS C SECH H T SC CI CL SP DI AIR MAT BT TURED COO DO PH SALH P 101 6/15/78 0 0 0.0 0 0.0 0.0 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 102 6/15/78 0 0 0.0 0 0.0 0.0 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0

- REL LS TAXA X S.E. AB%

1 2 0 CYANCPHYTA 17210288.0 45601568.0 31405920.0 14195640.0 97.9 0 CSCILLATORIACEAE O lyt:C8fA 7722994.C0 2200S432.0 16865713.0 7142719.00 46.4 O L1HCSYA (LPIL) 7722994.00 2200S432.0 14865713.0 7142719.00 46.4 0 RIVULARIACEAE O CALOTHRIX 9487300.00 23593136.0 16540218.0 7052918.00 51.6 0 CALoiHRIX (LPIL) 9487300.00 23593136.0 16540218.0 7052918.00 51.6 0 CHLCRCFitYTA 66577.50 946091.00 506334.25 439756.75 1.6 f

e 0 CHLOROCOCCALES O SCD.EDE!MUS 66577.50 946091.00 506334.25 439756.75 1.6 0 CUADRICAUDA 0.0 946091.00 473345.50 473045.50 1.5 0 EC01HIS 66577.50 0.0 33283.75 33225.75 0.1 0 BACILLARIOP.tTTA-FD MATE 66577.50 236522.75 151550.12 64972.62 0.5 0 FRAGILARIALES O DIATOHA 0.0 118261.37 59130.69 59130.69 0.2 0 TDUL 0.0 118261.37 59130.69 59130.69 0.2

, 0 NAVICULALES 0 NAVICULA 66577.50 0.0 33228.75 33288.75 0.1 0 44AVICULA (LPIL) 66577.50 0.0 33288.75 33288.75 0.1 0 BACILLARIOPHYTA-PD01 ATE (LPILI 0.0 118261.37 59130.69 59130.69 0.2 8 TOTAL 17343440.0 46784160.0 3:063792.0 14720360.0 100.0 DIVERSITY (H PRIME) 1.06 1.17 1.11 0.05 OIVERSITY (J PRIME) 0.53 0.50 0.52 0.01 IAJH81R OF TAXA 4 5 7 ABOVE ConPUTED USIIG SAMPLE IDS i 101 102 DATE 09/22/78 g PAGE NO 3 T6004QUA 9/28/77 i

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HCETHERN DOI At4A PUBLIC SERVICE COttPANY (49720) O BAILLY CD4ERATDG PLANT PERIPHfTOH CDtSITY DEHSITY IH MJt1BER / SQUARE CENTIMETER REPLICATE REPCRT PC TC GC LOC 5 66 66 0 0 40 DUPATICH TOW $171P VOL WDC CURENT T Ef1P SP D UNITS C SECH W T SC DI CL SP DI AIR WAT BT TURED COND DO PH SALN P SID DATE TIttE D/H UNITS C $3 WD 0.0 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 121 6/15/78 0 0 0.0 0 0.0 0.0 0.0 0 0 0 0.0 0 0.0 0.0 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 122 6/15/78 - EEL X S.E. AD%

LS TAXA 1 2 53026896.0 78329632.0 65673256.0 12651365.0 99.4 0 CYAHOFHYTA 0 OSCILLATopIACEAE 0.0 37985S0.00 1899290.00 1894293.00 2.9 o CSCILLATORIA 1899290.00 1891290.00 2.9 0 OSCILLATORIA (LPILI 0.0 3793580.00 53026896.0 74531056.0 63778976.0 10752080.0 96.6 0 LU4CSTA 56.6 53026c96.0 74531056.0 63778976.0 10752030.0 0 LUiGBTA (LPILI 231345.44 231385.44 0.4 0 CHLCPCF1YTA 462770.87 0.0 0 ULOTRICHALES 2313S5.44 4 2770.37 0.0 231305.*4 0.4

?

cb O

0 ULOTHRIX ULOTHRIX (LPILI 462770.37 0.0 2113GS.44 231385.44 0.4 66110.12 213403.37 139755.75 73646.62 0.2 0 BACILLARIOFHYTA-PEtatATF 1 0 FRAGILARIALES 53350.64 53350.84 0.1 O FPAGILARIA 0.0 106701.69 0.0 106701.69 53350.84 53350.84 0.1 0 VfUCHERIAE 0 HAVICULALES 33055.06 0.1 NAVICULA 66110.12 0.0 33055.06 0

66110.12 0.0 33055.06 33055.06 0.1 0 NAVICULA (LPILI 53350.84 53350.84 0.1 0 BACILLA1ICP!iVTA-pet 04 ATE ILPIll 0.0 106701.69 53555776.0 78543008.0 66049392.0 12493616.0 100.0 TOTAL 0.09 0.31 0.20 0.11 g DIVERSITY (H PRINC I 0.10 0.05 0 CIVERSITY (J PRIMEl 0.05 0.15 3 4 6 g NUMBER C? TAXA 3

0 ABOVE COMPUTED USING SAMPLE IOS O 121 122 s

O DATE 09/22/7S k0 PAGE NO T600 AQUA 9/28/77 0

l e a

T S

O 3

. J

tORTHERN ItcIAtaA PUOLIC SERVICE CONPANY (49720)

BAILLY GENERATitG PLANT P!RIFitfT0f4 DENSITY DEtt3ITY Iti HUMBER / SGUARE CENTIMETER REPLICATE REPORT PC TC GC LOC 566660050 DURATIOf4 TOW SAltP VOL WIto CLG ENT TEMP SID DATE tit *E D/H UtlITS C 50 WO SP O UNITS C SECH W T SC DI CL SP DI AIR HAT BT TURB3 COND 00 FH SALH P 251 6/15/78 0 0 0.0 0 0.0 0.0 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 252 6/15/78 0 0 0.0 0 0.0 0.0 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 REL LS TAXA X S.E. AB%

1 2 0 CYANCFHTTA 87160240.0 10952238.0 49056224.0 38104000.0 97.0 l 0 OSCILLATORIACEAE O 00CILLAT09IA 965357.12 0.0 492678.56 492678.56 1.0 0 03CILLATORIA (LPIL) 985357.12 0.0 492673.56 492670.56 1.0 0 LYtiGLYA 86174896.0 10952238.0 48563552.0 37611328.0 96.0 0 LYNGBVA (LPILI 86174896.0 10952233.0 43563552.0 37611328.0 96.0 0 BACILLtRIOPHfTA-FEl#44TE 2496237.00 578034.69 1537135.00 959101.12 3.0 ts 0 FRAGILARIALES 8 0 DIATottA 1072944.00 283946.87 678445.44 394498.56 1.3 0 TEl.tJE 10 7294 4. C 0 233946.87 678445.44 394498.36 1.3 0 ACHilA!4THA LES 0 ACitt 4HTHES 985357.12 0.0 492678.56 492678.56 1.3 0 NItRJTISSIMA 985357.12 0.0 491678.56 492678.56 1.C 0 NAVICULALES 0 #4AVICW. A 109464.12 0.0 54742.06 54742.06 0.1 0 HAVICULA ( LPIL) 109484.12 0.0 54742.06 54742.06 0.1 0 COMPHot EMA 328452.37 223101.12 275776.75 52675.62 0.5 0 OLIVACEUN 328452.37 0.0 164226.19 164226.19 0.3 0 GottFHONEMA (LPILI 0.0 223101.12 111550.56 111550.56 0.2

, 0 BACILLARIOPHYTA-PENNATE (LPIL) 0.0 7C986.69 33493.34 35493.34 0.1 8

l l

g TOTAL DIVERSITY IH PRINE) 89636400.0 0.32 11530268.0 0.36 50593328.0 39063056.0 100.0 0.34 0.0*

l h DIVERSITY (J PRINE) 0.12 0.18 0.15 0.03 9 FC."4ER CF TAXA 6 4 8 i AE0VE CottPUTED USItG SAttPLE IDS I

251 252 I DATE 09/22/78 h PAGE NO 10 g T6C0 AQUA- 9/28/77 I

1 I,V l

i i

NORTHERN ItiDIAHA PUBLIC SERVICE Cot 1PANY (49720 8 o

BAILLY GENERATING PLANT PERIPHYTOt4 CENSITY CEHSITY IH IAktBER / SCUARE CEHTIttETER REPLICATE REPORT PC TC GC LOC 5 66 66 1 0 10 DURATI0tt TOW SAMP Y0L WIto CURET 4T TEttP WD SP D UllITS C SECH H T SC DI CL SP DI AIR WAT BT TURBC Cota DO PH 5ALH P SID DATE TIME D/H UNITS C SD 0 0.0 0 0.0 0.0 0.0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 171 6/15/78 0 0 0.0 0 0.0 0.0 0.0 0 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 172 6/15/78 0 0 0.0 0 0.0 0.0 - REL

)f S.E. ADX LS TAXA 1 2 0.0 1153.4C 576.70 576.70 0.3 0 UNIDENTIFIED ALGAE 576.70 576.70 0.3 0 4X41DEHTIFIED ALGAE ILPIL) 0.0 1153.40 48389.34 22376.01 35332.68 13006.67 18.9 0 CHLCT!OPH (T A 0 CHLORCCCCCALES 31018.82 9803.92 20411.37 10607.45 10.9 0 SCEl.EDESitus GUADRICAUDA 31018.82 9803.92 20e11.37 10607.45 10.9 0

0 CEDOG0HIALES 13234.69 12572.09 12903.39 331.30 6.9 0 O! DOG 0t4IUM OEDCGC'4IUt1 (LPIL) 13234.69 12572.09 12903.39 331.30 6.9 ts 0 8 0 ZYGHERIATALES 2067.92 0.0 1033.96 1033.96 0.6 0 COST 1ARItX1 COSitARit;tt (LPIL) 2067.92 0.0 1033.96 1033.96 0.6 0

STAURAS1Ptki 2067.92 0.C 1033.96 1033.96 0.6 0

STAURASTRUM (LPIL) 20$7.92 0.0 1033.96 1033.96 0.6 0

0.0 1035C.62 5193.31 5190.31 2.8 0 BACILLARIOPHVTA-CENTRIC 0 EUP00! SCALES EUFCO:3CALES (LPIL) 0.0 10380.62 5190.31 5190.31 2.3 0

0 BACILLARIDPH(TA-pet 04 ATE 278548.87 12687.43 145618.12 132930.69 78.0 0 FRAGILARIALES 0 DIATCtlA 16543.37 0.0 8271.68 6271.68 4.4 0 TENUE 16593.37 0.0 6271.68 8271.68 4.4 0 S Yt.E DR A 49009.73 0.0 24504.87 24504.87 13.1 l 49009.73 0.0 24504.87 24504.87 13.1 0 SYNEDRA (LPIL) 0 EUt:OTIALES 0 EUNOTIA 2067.92 0.0 1033. % 1033.96 0.6 0 PECTINALIS 2067.92 0.0 1033.96 1033.96 0.6 0 ACHHANTHALES 0 ACHMAHTHES 210927.94 6920.41 108924.12 102003.75 58.3 0 NII:UIISSitl4 210927.94 0.0 105463.94 105463.94 56.5 6920.41 3460.21 3460.21 1.9 g 0 ACHHANTHES (LPIL) 0.0 g 0 BACILLARIALES S 0 NITZCHIA 0.0 3460.21 1730.10 1730.10 0.9 g 0 HITZCHIA (LPIL) 0.0 3460.21 1730.10 1720.10 0.9 0 BACILLARIOPHYTA-PENilATE ILPIL) 0.0 2306.81 1153.40 1153.40 0.6 g

O TOTAL 326937.87 46597.46 186767.62 140170.19 100.0 DIVERSITY (H PRittE) 1.68 2.50 2.09 0.41 DATE 09/22/78 PAGE NO 18

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NORTHERH ItCIAJ4A PUBLIC SERVICE COMPANY (497201 O BAILLY GENERATDG PLANT PERIPHYTOtt DENSITY DENSIT1r It3 NUf1BER / SQUARE CENTIMETER REPLICATE REPORT PC TC GC LOC 566661020 DU2ATICH TOW SAMP VOL WDC CURENT TEMP SIO DATE TIME O/H UNITS C 50 WD SP O UtIIT.) C SECH W T SC DI CL SP DI AIR MAT BT TIDDD CCtc CO PH SAlti P 0 0 0.0 0 0.0 0.0 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 191 6/15/78 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 192 6/15/78 0 0 0.0 0.0 0.0 0

- REL TAXA X 5.E. Ab:C LS 1 2 0 Ut4ICDITIFIED ALGAE 2506.43 0.0 1253.22 1253.22 1.6 0 Ut4 IDENTIFIED ALGAE (LPIL) 2506.43 0.0 1253.22 1253.22 1.6 0 CYAf:OFHTTA 0.0 75516.12 37758.C6 37758.06 48.7 0 OSCILLATORIACEAE O LVI.53YA 0.0 75516.12 37758.06 3775%.06 48.7-0 L)trAYA (LPILI 0.0 75516.12 37753.06 37753.06 48.7 0 CHLOROPHfTA 0.0 57541.08 28790.54 26790.54 37.1 C1 0 CHLOPOCOCCALES 15103.23 7551.62 7551.62 9.7 h

C) 0 0

SCEt:EDEStTJS ACUTUS 0.0 0.0 15103.23 7551.62 7551.62 9.7 0 CEDOGot4IALES 0 OEDCGCJ41U3 0.0 42477.84 21238.92 21233.92 27.4 0 OEDOCCttIUtt (LPILI 0.0 42477.84 21238.92 21233.92 27.4 0 B ACIL LARIOF HYTA-pdc 4 ATE 626.61 18879.04 9752.82 9126.21 12.6 0 ACHILAt4THALES 0 ACHHA!4THES 3.0 18379.c4 9439.52 9439.52 12.2 0 MItrJTISSIMA 0.0 18879.04 9439.52 9439.52 12.2 0 BACILLARIOPHVTA-PD44 ATE ELPILI 626.61 0.0 313.30 313.30 0.4 TOTAL 3133.04 151976.00 77554.50 74421.44 100.0 DIVERSITY (H FRIME) 0.72 1.72 1.22 0.50 l 0.72 0.86 0.79 0.07 DIVERSITY (J PRIME) t3JhCER OF TAXA 2 4 6 S ABOVE ConPUTED USIIG SAMPLE IDS 191 192 i DATE PAGZ IC 09/22/78 21 T600AGUA 9/28/77 I.

I .

3

\m Q ,g

HORTHERil INDI AftA PU6LIC SERVICE Cor: pat 4Y (49720) o BAILLY GE!4ERATING PLAlli PERIPHYTote CEtiSITY DE!4SITT Iti 14Ut;CER / SQUARE CEtiTIt1ETER REPLICATE REPORT PC TC GC LOC 5 66 66 1 0 30 CURATI0t3 TOW sat 1P VOL WIta CURET 4T TEt1P SID DATE TittE D/tt(MITS C SD WD SP D UNITS C SECH H T SC DI CL SP DI AIR WAT BT TURBD C0tc DO PH SAlti P 211 6/15/78 0 0 0.u 0 0.0 0.0 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 212 6/15/78 0 0 0.0 0 0.0 0.0 0.0 0 0.0 0 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 REL LS TAXA X S.E. ADX 1 2 0 CHLOROPHYTA 284834.62 0.0 142442.31 142442.31 58.9 0 CHLOROCOCCALES 0 CHARAC1Utt 177462.87 0.0 83731.44 88731.44 36.7 0 Are!GUL, tt 177462.87 0.0 08731.44 86731.44 36.7 0 CHAETOPHORALES 0 CHAETO?itOR ALES C" 92507.31 0.0 46253.66 46253.66 19.1 0 CHAETOPHORALES **C" I LPIL) 92507.31 0.0 46253.66 46253.66 19.1 e 0 CEDOGO!11 ALES 8 0 OE00CO 41 Ult 14 9 M .4/* 0.0 7457.22 7457.22 3.1

[ 0 OEC000'11Utt iLPILi 14414.44 0.0 7457.22 7457.22 3.1 0 BACILLARIOP:tfTA-PEtolATE 1963*2.00 2690.C8 9531n .4*e 96625.50 41.1 0 FRAGILARIALES 0 01ATCitA 1887.90 0.0 943.)5 943.95 0.4 0 VULGARE 1887.90 0.0 943.95 943.15 0.4 0 ACHtlAt4THALES 0 ACHilANTHES 11327.43 1169.95 6248.68 5078.74 2.6 0 Lt!1CEOLATA 11327.43 0.0 5663.71 5663.71 2.3 0 ACHNANTHES ( LPIL) 0.(- 1169.05 534.97 584.97 0.2 0 flAVICULALES 0 GotiPitCt4Et1A 5663.71 233.99 2948.85 2714.86 1.2 g 0 CLIVACEUtt 5663.71 0.0 2831.86 2831.06 1.2 O GCitFH0tlEtlA ( LPIL) 0.0 233.99 116.99 116.99 0.0 8' 0 EPITHET 1IALES lg 0 0

EPITiitt1IA TURGIDA 3775.81 3775.81 116.99 116.99 1946.40 19 4.40 1814.41 1029.41 0.8 0.8 0 BACILLARIOPHYTA-pet 04 ATE ( LPIL) 173687.19 1169.95 87428.56 85258<S6 36.1 TOTAL 481226.37 2690.E8 241c58.62 239 M.7.6 9 100. 0

<e DIVERSITY IH PRit!El 1.95 1.55 1.76 9.7%

h DIVERSITY (J FRIt'E l 0.65 0.77 0.71 2 .f4 3 HullSER OF TAXA 6 4 10 b A00VE COMPUTE 0 USING SAttPLE ID'S I. 211 212

• e I DATE 09/22/78 3 PAGE HQ 23 T600 AQUA 9/28/77

i

)

APPENDIX E ,

ta h ZOOPLANKTON DE!.:.itT REPLICATE REPORTS JUNE 1978 t

I k.

I

.r I

f 3

LREPCRT ILf3IT 8.

LABEL 1='ICRTHEPte IPCI At3% FL'BLIC SERVICE Cor1 fat 3Y (49700l*.

LADEL23'BAILLY GEt4 ERAT 1t4G PLA!1T*.

L ASE L 33 *lCOPL AP:NTCt4 DEt3SITV ' .

RLABl** REPLICATE REPORT *.

RLAD2a'STATI0tl REPORT *.

RLA832'STATICta REPORT *.

RLtB4 ' SITE SUt? TART'. [

SPEC 1:4.999.5. 'R At4 * .

• Y ES * . 3.4 SPEC 2:3.999.4.* RAP 4'.*YES*.3.7.

SPEC 3 7.999.4.' rat 4'.*YES*.3.7.

S PE C4 : 6. 99 9. 6. ' R Art' , ' Y E S * . 3. 3.

m REPTOT:60.

I LSTAT 1.

N4XTXA:500.

GEARTD:51.1.0.

ISIZEz2280 i

LIST *2.

SLRtLEV:1.-l.0.0.0.

JCT:35.

STLRAIT:20.

t1 LIST:0.

4 51 FILE:1.

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BAILLY GENERATItG PLANT 200PLAkETON DEttSITY REPLICATE REFORT r

PC TC CC LOC 5 51 51 0 0 00 DURATICH TOW SAMP VOL WIPC CtRENT TEttP SID DATE TIME D/laLR11TS C SD WD SP D Ut31TS C SECH W T SC DI CL SP DI AIR WAT BT TURED C0tD DO PH SALH P 21 6/14/78 0 0 0.0 0 0.0 0.0 0.0 0 7.1 3 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 22 6/14/78 0 0 0.0 0 0.0 0.0 0.0 0 7.1 3 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 23 6/14/78 0 0 0.0 0 0.0 0.0 0.0 0 7.1 3 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 24 6/14/78 0 0 0.0 0 0.0 0.0 0.0 0 7.1 3 0.0 0 0 0 0 0 0.0 0 0.0 0.0 0 0.0 0 0.0 0.0 0.0 0 REL LS TAXA X S.E. AB%

1 2 3 4 0 OLIGOCH4 ETA (TOTALI 0.0 0.0 93.90 0.0 23.47 23.47 0.1 0 13AIDIDAE 1 NAIDIDAE ILPILI 0.0 0.0 93.90 0.0 23.47 23.47 0.1 0 ARACliNID A I TOT AL D 0.0 46.95 0.0 0.0 11.74 11.74 0.1 0 FROSTICt1AT A 1 HyDRACPRINA (LPIL) 0.0 46.95 0.0 0.0 11.74 11.74 0.1 0 CLADOCER A (TOTALI 2136.15 4553.98 4eS2.62 4916.77 4100.33 667.11 19.8 0 BDSMIffIDAE Y

e 1 200MIttIDAE ELPIL) 1854.46 3285.38 3568.07 4353.39 3265.58 521.74 15.7 O CHIC 091DtE 1 ALetl\ FECTtN3t'LA 70.40 0.0 375.59 153.65 149.91 81.51 0.7 1 CH1CO2US f tPIL) 211.27 1200.66 845.07 409.73 671.68 225.84 3.2 1 EURICEP US LAt!ELLATUS 0.0 0.0 93.90 0.0 23.47 23.47 0.1 0 0 4THillt A E 1 DArHt:IA ArmIGUA 0.0 46.95 0.0 0.0 11.74 11.74 0.1 0 COPEFCD4 (TOTAll 6931.4G 235:1.10 27 30.03 8860.43 16608.24 51 2.33 80.0 0 C ALI.t:3IDA (TOTAL) 1 DI APTCt1US ASHLAllDI 0.0 93.90 93.90 0.0 46.95 27.11 0.2 1 EL"ffTEt!CRA AFF1HIS 23.47 93.93 0.0 0.0 29.34  : .22 0.1 14 CAL #UOICA (trIL) 399.06 1067.60 1314.55 1075.54 1014.19 g 0 211.46 4.9 CICL0f 0!DA ( TOTALI O 1 CICLOPS BICUSPIDATUS THOMASI 234.74 1032.26 751.17 056.08 568.71 195.36 2.7 1 CTClor3 VEEtiALIS 0.0 187.79 375.59 51.20 153.65 83.93 0.7 14 CTCLOPOIDA ELPIL) 6244.12 20S45.05 24694.82 7477.59 14015.39 46E6.10 71.3-TOTAL 9037.54 281 2.02 13777.00 32 06.55 20785.82 5563.24 100.0 DIVERSITY (H FRIME) 1.38 1.39 1.32 1.65 1.43 0.07

. DIVERSITY IJ PRIME) 0.49 0.42 0.40 0.59 0.47 0.04 I4Utt3ER OF TAYA 7 10 10 7 13 ABOVE COMPUTED US!!G SAMPLE IDS 01 22 23 24

{ DATE 09/02/78 g PAGE N3 3 0 T600AcuA 9/28/77 3

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